LOCUS       DAOQNQ010000001        50753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_190_length_50753_cov_7.208391, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000001 DAOQNQ010000000
VERSION     DAOQNQ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 50753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50753
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_190_length_50753_cov_7.208391"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4817)
                     /gene="sprA"
                     /locus_tag="PK754_00005"
     CDS             complement(<1..4817)
                     /gene="sprA"
                     /locus_tag="PK754_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNB07914.1"
                     /translation="MRLLIHQYDLQVGGLFLHIISKRFLVVSFLLFFLNPSKSFSQDL
                     DSISVSLQTADSILFFINEKDIEKNINKTPTSKDVRQDSVWSDLNAIKALETELISLM
                     SEINANLSITGQLDLVINQVIENEAKKIKDSRSTRSLFSLYPFQYHNTISIDPNQNYF
                     LIRGGSIDFSQTQKLLPGYKVIAMLDSSGNYVFADEFGQTTKKQRYKETTVIKQESYI
                     SNSFVYQIRSVFTKTVVTGIASKEERQSGAVRLFSASVTTNETFQKIFGGDEVTVDAT
                     GNINLNVSGASEKSSNQSSSTGKSSQFIPKFEQQQKFNLRGTIGKKVEILFDQDSERE
                     FSFENNIKITYTGFDDEILQKLEAGNIDLSLPGTQYVTQGGQNKGLFGVKSIFKFGDL
                     TLTSIASIQNGEKKVIEINPGSGNNSTSIIKKSAKDYEKGKFFFLDKRYLNRYEMYDP
                     QDGRKHTYDPADEIINIRLFRKSEGNDRNPEKRIGIVTYNNSTLTNPNLPNMTVSQIT
                     DLDSANAATMIFLEIPKEQFSCNFQLGIINLSTKLTVGEALAVYYETSQQVFGDVSSD
                     TLKLKLLWKPTPLPTDSTWDLELKNIYAFDGITGLSAEEGESVKIKFKPSSNNDITSF
                     PNIGGKDRGLLDILHVDETSQNGKSKDPDQIIDIAPSSTVLMGYGLDYDNGWIIFPRL
                     RPFDPPTTPLPGFRDEVFPALNNDPNLGEVRIPIIYNEARSSDKFKQFNDRYSIEIES
                     KKSSSAKFSLGINVLESSEVVRLDGDVLEKNIGYTIDYTSGELILIDPRAALSTANIK
                     IEYEQAQLFQVDKKSIFGTRAEYNLANYGFGSNSFIGTTALFHSQSTVNKRVQINEEP
                     FNNFVWDINTNIELEANYLTKFINYIPFVSSNQNSKINFAAEYAKLLPTPNTSNGLME
                     NDENGVAYIDDFEAIKRTYPMGSNRKSWTLASKPIEKNASKRRKLIWYQNEISRSKIS
                     NIKTNNTDRATVLTLTLQNKISDEAPTEGLWGGVMRGFSLSSAGELGASRFIEFWVKN
                     NTPEGKHAKINIEIGEISEDQNGNGGAPNREVKSALQPVIEKNQDIGLDDLSDAGEDS
                     LLSKLGITRSQYGNLSFSQKAIVDSLQIIFPWGTIALDPDDPFGDNWNNEQARFGNKD
                     IQNAEANRDIRNIKVNGYENNTFPITDGGLRIGDTEDINNNGVIDIQNVYARYSVTTD
                     THSLDNDIIVGRGEDGWILYRLPILRPDTIINSNSIDLVLPKITSARIWISSEDPESE
                     IISVQLSEIQFINSEWKLPIEVPNGVLLNPNGKIPSNNENSKIVEISSISTDEGGGYT
                     RPNGVKREKPIDQNGNNAQREVKEQSLLLRLNELPVNSKAIISKSNSQRDFRNYGRIR
                     MFVHGDKGGSIPLPISDESSDSPLKYFIRFADRFNSYYEIEQPLYAGWSEEKNSLDID
                     IRENLTSLKFQRTGNDTLTEYNIGDNKIIRIKGNPSLDNVQFIYLGVHNTSLVNAYTG
                     EIWFNELRVTDADDKSGEAVMARLSLNVGDMLTLSGSTEYRSADFRTVEQRFNPSGGN
                     QKSWSISGNLNLHKFHIERWGISLPLSFNASHNTSDPKFLPNNDILVTDAKEA"
     gene            complement(4936..5619)
                     /gene="rsmG"
                     /locus_tag="PK754_00010"
     CDS             complement(4936..5619)
                     /gene="rsmG"
                     /locus_tag="PK754_00010"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNB07915.1"
                     /translation="MQNNQINWDVFFQILENNTLKLSSIQKNQFFSFHDFLLDWNKKI
                     NLIGRQEGNIISRHFLNSALIAFFVKFKKEQKVIDIGSGGGFPAIILNIIFPETHFTL
                     VESIKKKSDFLKETSKVVGLKNLVVINERAEKIAKTKSFSSHYEFVTARAVSALDELI
                     PLTSPFLKNEGRMLFLKGKQVDIEVESVKKSNWPDKYTLTIHRFQNIPFIPSDQESAL
                     VDIKFFLDR"
     gene            complement(5623..7539)
                     /gene="mnmG"
                     /locus_tag="PK754_00015"
     CDS             complement(5623..7539)
                     /gene="mnmG"
                     /locus_tag="PK754_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNB07916.1"
                     /translation="MDVFDVVIVGGGHAGYEAAFAASRLGCSTLLLSMETTKIGTLSC
                     NPAIGGTAKGHLVKEIDSLGGEMGFITDQVGIQFRMLNKSRGPAVWSPRAQVDRAAYP
                     LKIQELLGLKKNISIRDANIVGVVVESNQIKGVITDKNEVIKGKTVILTCGTFLNGII
                     HIGLKNVRAGRIGEKPSLGITESLISLGFESSRLKTGTPPRIHKDSINFSVCEPQYGD
                     NPPEPFSYRTDSITNTQIPCHLTYTNDKTHDILKSGFDQSPMFTGRIKGLGPRYCPSI
                     EDKINRFSDKERHQIFLEPEGYDSIEYYVNGFSTSLPEEIQISAIRSVTGLENAHVLR
                     PGYAVEYDFFPPYQINLNMETKYVEGLFFAGQINGTSGYEEAAAQGMMAGINAALKVK
                     QKDPFILRRNEAYIGVLIDDLVNKSTLEPYRIFTSLAEYRLLLRQDNADMRLMEKGNA
                     FGLIDEKQMEKFIRKKDAINQIETWIEKTNLHPKKINSFLINKGSTILEEPEPLAKIL
                     KRPEVTLTDLLECEEEIFEPKIVNLLKYRDTLREVDTSIKYDGYLKRQQEQVDRFLRM
                     ENKKIPLNFDYDKITSLSKEAREKLKKVKPGNVGQASRISGVSPSDISLLLVFLERGS
                     LGKNIQDSFNDTLN"
     gene            complement(7693..9069)
                     /gene="mnmE"
                     /locus_tag="PK754_00020"
     CDS             complement(7693..9069)
                     /gene="mnmE"
                     /locus_tag="PK754_00020"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNB07917.1"
                     /translation="MNFDETIAAISTPIGTGGVCMVRVCGKEAVNLADQIFLGHSKPS
                     QHISHSLHNGYIIDPLTKEKIDHVLIGVMLGKKTYTGLDTIEIYGHGGVLNAKRILDV
                     CIKYGARHAEPGEFTKIAFLNGKMDLSQVEAVADLIHAKTESARRSSMAQFMGNLSHE
                     IDELKKQLIKLCSLLELDLDFGEENLINITKESIDAQITNVRLQIEKLLSTYNTGHLI
                     REGAKISIIGKPNVGKSSLLNAMLKRNRAIVSDIPGTTRDFIEESLDIEGIPFIFVDT
                     AGIRQSKDVVENQGIQSSMEIAKNSDLILVIFDGSEDLNEEDINILKMVEDLIWKQKH
                     VKALFIANKCDKENYNPQRTKNDFIEISAKNDIGIDKLKRSIGDLFLNNFSIESPMIT
                     RLRHKDALENSVVSLNNAKVSLHNKMSYEFISLDIKNAIRHLGEIIGEVTGDDVLNNI
                     FSNFCIGK"
     gene            complement(9144..10868)
                     /gene="yidC"
                     /locus_tag="PK754_00025"
     CDS             complement(9144..10868)
                     /gene="yidC"
                     /locus_tag="PK754_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNB07918.1"
                     /translation="MDRRTIFGLFMIALIIILYPFYMQAVAPPADPSVKTTLAVDSTS
                     TPTHPDTLLQSKTNISKESDLPKTVSLTKDTIKQSEKIIRINSEIYSAEISNLGGGSI
                     KSWKILPYKTHDGQPVDFIESSKNLNLNFVFQGKNYNTWNIPYSIINTDSVIDLITKQ
                     KNTLTFVYHFDSERQIIQNLTFYNDRYDVDIEVQFKNFGDDITNGEYQIQWLSALKST
                     ELDRNGDLSHTISYAYMGDEKEEFDIGTDPSKETTWSGVTKWVASRTKYFAVYIIPQE
                     KDGIGCKLTSTTTHENGLLTKKSNFMNIQMKFEPNVVDRYKFYIGPMDYHILKEYDDN
                     VAVTMEWGWAVFRPLTKAIFYTFNFLYDIIPNYGWVIIVFTLLVRIVLYPLTYKTFVG
                     MQKMKVVMPRQKEIQKKYKDNPSRMQQEMMKLYKEAGYNPLSGCLPMLIQMPLLFPIY
                     QVFNQSIELRLAPFFGWITDLSRPDTITILHTGLPFIGDFHVNPLPIIMTVLTFLQQK
                     IAPMTPMGDDSDPAQRFNQKFLMYGMPIMFFFLFNGFASGLVLYWTVFNVLTMAQQVM
                     MQKFVEVK"
     gene            complement(10890..11144)
                     /gene="yidD"
                     /locus_tag="PK754_00030"
     CDS             complement(10890..11144)
                     /gene="yidD"
                     /locus_tag="PK754_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010179022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNB07919.1"
                     /translation="MLRRSVLYFFVSLIRIYQHTLSVYFPSSCRFYPSCSQYSIESLK
                     SHGLFKGLWLTIRRISKCHPFHEGGYDPVPQNQNTNNKCC"
     gene            complement(11220..11363)
                     /gene="rpmH"
                     /locus_tag="PK754_00035"
     CDS             complement(11220..11363)
                     /gene="rpmH"
                     /locus_tag="PK754_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNB07920.1"
                     /translation="MKRTYQPSKRKRKNKHGFRSRMKTKGGKNVLASRRAKGRKKLSV
                     SDE"
     gene            12012..13400
                     /gene="dnaA"
                     /locus_tag="PK754_00040"
     CDS             12012..13400
                     /gene="dnaA"
                     /locus_tag="PK754_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNB07921.1"
                     /translation="MSAIIELDAKSVWNRCCDIIKSQIPGPSFRTWINPIEPHVIENE
                     KFVAFIPNQFFVEWLESRYNDLILNTLREVCGRSIGVTYIVKEITEPIQTVSTPSTPL
                     VLQASVNKPKETAPVVYDSFINIRYTFDNYIEGNGNKFAKAAALAVSEKPGKTSFNPL
                     VIYGGAGLGKTHLIQAIGNQVKRNFPEKKICYVTSETFTNDFVAAIRKNDVLEFSRLY
                     RSFDLLMVDDIQFFADKGKIQEEFFHTFNTLHQLGKQIILTSDRPPKELKQVEERLIS
                     RFQMGLVTDIQPPDLETRIAILQKRSEEDGVYLPPDVCEFVAMNITSNIRELEGSLIR
                     LLAYASITNTELNLDVAKAVLKDTIASKRKNLSIETIQKIVSDYYSIPVDQMLTKGRK
                     KEIAEVRQICMYLVKEILGSSLKTIGIHFGGRDHSTVIYACKMVEEWMKSNTKIKEDV
                     EIIKRKIELAAF"
     gene            complement(13470..14648)
                     /locus_tag="PK754_00045"
     CDS             complement(13470..14648)
                     /locus_tag="PK754_00045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007958704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNB07922.1"
                     /translation="MKEVFILSAVRTPIGKYGGVFSSIKAKELGIIAAAESIMKAGLT
                     PDSVDEAIFGCARQAGIGPNISRQIAVGVGIPSQKTAYTINKACGSGLKSIINAYTSI
                     VLGDNDVVLAGGVESMSNIPYLLTQARWGYRMGNSEILDANHTDGYFCPMADKLMGAT
                     VEDLALKYNITRDEQDAFALQSQLKAESAIKTGKFNNEIIPVKVATKKGDQIITTDEH
                     PRIGATIEDFKKLKPVFKKDGTITAGSSSGVTDGASALIIAGKEFVQNNNLKPLARII
                     GYASAGVNPSEMGIAPVPAVQKLLKKSNLRLQDIDLIELNEAFAAQALACDRELHFDK
                     DKVNVHGGAIALGHPTGCTGARITTTLVHSLHDRKAKLGIATLCISGGLGLALLIEKV
                     "
     gene            14762..15490
                     /gene="pgl"
                     /locus_tag="PK754_00050"
     CDS             14762..15490
                     /gene="pgl"
                     /locus_tag="PK754_00050"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNB07923.1"
                     /translation="MKTHTTVFTSADELAEKTAEKIISIGHQAISMHGHFSWSLAGGK
                     TPKMVYEVLAKKHKESIDWKKVHLFWGDERDVISTHPDSNFGMAKNVLMDPIGFPDAN
                     IHRIGTEEDCMLAAQQAEDDIKDFFGLSDGGFPKFDLILLGLGEDGHTASLFPGTDGI
                     TETKFLVMDNFVKKLGRHRVTFTFPTINHASNVLFLVSGKSKSSIIKQVLQGSTEDIL
                     PAQMVRPAGELYWYLDKEAASELQ"
     gene            15487..15927
                     /gene="tsaE"
                     /locus_tag="PK754_00055"
     CDS             15487..15927
                     /gene="tsaE"
                     /locus_tag="PK754_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNB07924.1"
                     /translation="MIPDYISHSEKETFDYAEDFAEKIKPGDIIALFGYLGSGKTIFA
                     KGLCKGLGVQEPVHSPTFVIVNEYEGYKNGKALIIRHCDFYRINTEKDIAELGIEEMM
                     DQEDYVVIAEWSERVEKKLPQSSWRVYFEKRNEQERQISIRREL"
     gene            15924..16577
                     /gene="tsaB"
                     /locus_tag="PK754_00060"
     CDS             15924..16577
                     /gene="tsaB"
                     /locus_tag="PK754_00060"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNB07925.1"
                     /translation="MKLLVLDASTSICGVALVEGNESEIKLISSIHLNINNAHSERMM
                     LLVEYIMQQSSISSKDIQAIAVTIGPGSFTGLRIGLSVAKGLAFSFKVPLVTVPTLEA
                     IALPWIDHYRRIWVALTSRKKEYYIAFFEDHNVLIKGQVWKFDELENNLLQGDLLLTD
                     DPISFSELSGIHISNSQDGLPNVFSVGKIGLTKAIAGIYADLDNVVPDYFQGFRGAS"
     gene            16622..17056
                     /gene="rimI"
                     /locus_tag="PK754_00065"
     CDS             16622..17056
                     /gene="rimI"
                     /locus_tag="PK754_00065"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNB07926.1"
                     /translation="MTNEDLDTVMRIEGASFDSPWTRSNFADELKNTDIALPLVLEQN
                     KLIIGFIVLWIILDECHLANIAVTPDYRGRGLGKLMMQNIISIARQNKCVKVMLEVRK
                     SNTTAIALYQKFLFEKVGVRKNYYHDGFMNTEDALLMDLNLI"
     gene            17073..17945
                     /gene="accD"
                     /locus_tag="PK754_00070"
     CDS             17073..17945
                     /gene="accD"
                     /locus_tag="PK754_00070"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNB07927.1"
                     /translation="MNWFQRKKDGVNESTEKRELPNVWRKCDKCGEVQYKKDVEKNYF
                     MCVKCGYHFRVFARTYIEIMLDENSFVETESNMISVDPFQFVDTKKYSDRIQAMIKKT
                     KIKDAIRTGYGTIEGRPVMVAIMDFGFIGGSMGSVVGEKISRAIDQAYKNKLPMIIVS
                     ASGGARMMEGALSLMQMAKTSAKLALLHEKGIPYISLLTDPTTGGVTASFAMLGDVII
                     AEPGALIGFAGPRVVKEATGKDLPDGFQTAEFLVEHGFVDLIVPRNELKKTLSRLFKF
                     FEPNTDTQFKPTKS"
     gene            17969..18733
                     /gene="surE"
                     /locus_tag="PK754_00075"
     CDS             17969..18733
                     /gene="surE"
                     /locus_tag="PK754_00075"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNB07928.1"
                     /translation="MKHILLTNDDGIDANGIFALYKELKKLGDVTVVAPQIERSAVGH
                     AITIHDPIRVLDYHRPEKFKGYAISGTPADCVKIGVKNLVHKKPDLIVSGINHGSNTA
                     TNVLYSGTVSAATEGIIMGIPSIASSMIAFDHKTDLTFAAEITAKIARIVLDKGLPSD
                     TLLNINIPPGPRTSIKGIAVTRQGRGRYEEEFEQRTDLSGRAYYWLTGKKMILDHEDD
                     VDDVVVLKNKVSVTPIQIDLTAHKFLDELKTWNIEI"
     gene            18828..19373
                     /locus_tag="PK754_00080"
     CDS             18828..19373
                     /locus_tag="PK754_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNB07929.1"
                     /translation="MKKTLRYLAFSLMLSGFVFSQDNKKPSPPAKSEQKVGSEKISIN
                     YSQPSVKSRKIWGELVPFGLTKNRDGKMIPWRAGANENTTFTVSKDVKIEGQTLKAGT
                     YGLHMIPGESEFVVIFNKSSTAWGSYSYDQNEDALRVTVKKETAKFQEVLTYGFESVT
                     DKSCTVYLHWELVKIPFKIEF"
     gene            complement(19472..22168)
                     /locus_tag="PK754_00085"
     CDS             complement(19472..22168)
                     /locus_tag="PK754_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07930.1"
                     /translation="MEYLPSSKNPIHSQRRRGKFYDVSDILAQKPSTISGEDIQFFEE
                     LDLVYRTMCGILYNFVPLSGHPGGSLSSGRIVEGLLYQSMDYDFSKPDIDINDMICYT
                     AGHKAMGLYAMWALRNELIRIANPGMLAPERRQLRLEDLLGFRKNPTQGTPLFEKFKS
                     VSLDGHPSCIVPFVKTATGPSGVGVPAAFGLAFGALDMYPENPPRTHLLEGEGGMTPG
                     RVYEAIAAAASAQLRNAVLHIDWNQSTIDSDRCTAEGDKHGDYVQWDPVELMYLNDWN
                     VIFVPEGHNYHQVLTAQKIALSLQSDMPTAIVYRTVKGYTYGVTGKASHGAGYKFCSP
                     EFYNFIKPFEEQFGVMFPRFSGDAKNPVDVETFYWNTLLVIREVLEKNSFLPQEAAKR
                     LALSKDRLYSHNRTHRAHAPKLANIYDGTVNLSAMPDDIRLEPGTSTTIRGQVGKVLN
                     YLNKKSNGALVATAADLFESTSIAAVGKGFPDGMFNAKTNPGARLVPVGGICEDAMGA
                     FMSGVSSYRNHIGISASYAAFIVAMEHTAARCHSIGQQNYWERTGEPMHTWIMVNGHA
                     GPKTGEDGPTHADPQCLQLLCEDFPSGKLITLTPWSPDEVWPLMIEGIMKRPAVLAPF
                     VTRPAEAVPDRVKLGLDPAIAAAKGVYAFRRANMNAREYHGTLVLQGCGVASMFINGV
                     LEKIDKAGLNMNIFYVASTELFKLLPEKEQEAIFPEHLRTHAMGLTDFTLPTMYQWIR
                     WNEGLNRTLHPFRHGHHLSTGSAEKVLEEAGLHSEGQFKEIMKYARMIEKMNLTKPQT
                     DSVKAEEKIRSNRGRPKGYKTKVARVRTTKPAKQQIKNTRAKSNEHINRNKKAQTKIT
                     IKPGPGRPKKNKLVRLKLKSQTQKKSQTTKKIKPGPGRPRKK"
     gene            22261..23433
                     /locus_tag="PK754_00090"
     CDS             22261..23433
                     /locus_tag="PK754_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HNB07931.1"
                     /translation="MPKPEKHEEKSDSYRIVKYESDEASEYNDFEIAPQTPFTTNIKG
                     RSSGFNPPNRFDTQHQDKSIDDITEEDIDPERTVPTELIEDKSRTILSKNDSPDIPFT
                     YGINPYRGCEHGCVYCYARPSHEFLGHSLGLDFETKIHVKYDAAKLLEKEFLKPSWKA
                     EPIAFSSNTDCYQPIERKLKITRACLEVFLKYRNPVRMITKNYLITRDLDIISQLSKL
                     NLFGAMISITTLDTGLARRLEPRTSRPDNRLKAIELLAKNNIPVGVLTAPIIPGLNDH
                     EIPEILRRAADSGATSAGYTVLRLPYQLRELFSDWLTRNEPNKKEKILHAIQSVRGGK
                     LNNTEFGKRMRGEGNMADHISNLFNLFYKKYGFDKKVVRGEPASFLRGGVVQESLF"
     gene            23457..24101
                     /locus_tag="PK754_00095"
     CDS             23457..24101
                     /locus_tag="PK754_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNB07932.1"
                     /translation="MNWNDALMPIIKKYKNKKHPLEYKNRYQLLVMTLLSAQDSDKHI
                     NEVGSRFFGVYPSMRELAKATEDEIASHIRDVRNFGNKAKWLAGIAKTVGSDENIPTT
                     METLTSLKGIGRKSASVIIRESGGPAEGIIVDLHVVRVAPRVGVTKEEKPEKIEKDLM
                     AVVEKKYWGDVGMAISFLGREICRPTNPKCEECPINENCSYFQNQIRSAKSKKN"
     gene            24189..24962
                     /gene="bshB1"
                     /locus_tag="PK754_00100"
     CDS             24189..24962
                     /gene="bshB1"
                     /locus_tag="PK754_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013752401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HNB07933.1"
                     /translation="MKLDALFFGAHPDDVELSAGGTVAKLVKLGYKVGICDITRGEAG
                     TRGSAEMRDKEAEDAAKILGVHVRENIELPDTEFVNDRANQLKAIHIIRKYQPEFVFC
                     QYWNDRHPDHMHASDLVRESSFYSGLSKIETSDKGKPQEKFRPAKVIYYAARYEFSRI
                     DGHIFIVDITETIDIKMKAMAAFASQFFNPTYKSDEPQTYISSPEFTDAIRNRAAHFG
                     SLIDTKYGESFLVREFPSVPDPVKFFRDGGQDVNKLGLA"
     gene            25004..26035
                     /locus_tag="PK754_00105"
     CDS             25004..26035
                     /locus_tag="PK754_00105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB07934.1"
                     /translation="MNTKISIDDLFERIQLVKKINAEQPLFPSYEKYFDRKINTMSSV
                     SKLNRKLLAWDTILRYHGIDLENKKVLVVGCGAGWECVYMYAMGAKEVVGLEVMEMFV
                     KGMEKMFPILGLHPTVKPILQDIHTYSLDSYYDLILTVDAISHMYDHKLFLSSCFKLL
                     APNGSLLIIDDNNALNYRRRKEVANIWESWEKYGLDLKSNDGKTMHINSYEQDRYEYI
                     VERFPELSKEAKSLAERTANFNRQQIDNAILKYKKTGIMPNSVYRRGVMAYDSKYDMP
                     KEKQFNPYTLAHEIRSARFENAIHLPDLITTDTFFRKNVVKMLIVFKPFSYFFVSKGF
                     SVSGRKSSH"
     gene            26054..27226
                     /locus_tag="PK754_00110"
     CDS             26054..27226
                     /locus_tag="PK754_00110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HNB07935.1"
                     /translation="MQICIYEDDHYVNLYPLTLTRPVFELRCGASTLAERIARYFPEA
                     EIFYQCRPLLASVYHEQYPDRRINHKISGECLFINGRVLMNDALMQQMSEHSGGEFIN
                     QGVIVGFWNSVKPVKLDAETRMIKYPWDLVKYNGECLREDFDRNKSGHTQATIYAGVH
                     LVNADAITIGEGSSIKPGVVLDAEHGPIMIGKHVQVMPNTVIEGPCYIGDHSVIKIGA
                     KIYGSTSIGPVCKVGGEVEGSIIQGYSNKQHDGFLGHSYIGEWCNLGADTNTSDLKNN
                     YGPVSVTINGAEINTGSLFVGLTMGDHSKSGINTMFNTGTVVGVCSNVFGGGFPPKVI
                     PSYAWVDSPKITLYKMDKAMQVANTVMARRKVTLGEKYNLLLQWIFEFAKDEHAKL"
     gene            27223..28140
                     /locus_tag="PK754_00115"
     CDS             27223..28140
                     /locus_tag="PK754_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07936.1"
                     /translation="MNEWLKNKKFIVKALGALILVLLTVHLIISITRVSKQEDPFAYI
                     KEEPVAEQVIPEPRRLTIGVSLEQLNRYVPFDEAIDSLLTHEFDIQPGFIKSERIQLE
                     NVNYIRKVIQLPKNFPNTLFVLDMNVLSEQRDWDIFSVSEKLNRQAGASDFTMDIGRR
                     NLIYSRLEFTVNPRLRPMGKEVFFVVTGFGKNFDDLTRSFVELPESFAILVPKTESNY
                     KIIAHEAERTGKSVMTYMPWSKYIYYFDEKGGDQERLTQALYENLEKAANRAFIVCEY
                     SSATYQVLKKELPRLAKKGYIPRTYIQKW"
     gene            complement(28318..29031)
                     /locus_tag="PK754_00120"
     CDS             complement(28318..29031)
                     /locus_tag="PK754_00120"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07937.1"
                     /translation="MNRNKFYLFLILFFLESCSTGFPEIPPGATHKFHATDFPDSFVL
                     LTVPNKYESKRPTPVLIALHGSGDRAEAFHDFWKKSADSLGLILVTPQGLEKMNMGFG
                     WNRNEATERYIKNVLVDVRKRIQIDSTQIYFAGFSSGGSLAAYLTLKNTIPNRGTVLF
                     GASLDTHSSLFNSGEKARFYIGHGTLEQNYPHISSVADTLAQSGHSVRFVPYPNIGHT
                     IPEPKQKELLNALRWILEK"
     gene            complement(29018..30133)
                     /locus_tag="PK754_00125"
     CDS             complement(29018..30133)
                     /locus_tag="PK754_00125"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNB07938.1"
                     /translation="MKIFVILCMVLCTSIPSVSQVPIDRHDGIKVSDASSMHILPTTL
                     LKLDSAANQGVLKNITSILIARHGTLVFENYYNGADEATLHNTRSATKTITAILTGLA
                     IDQQFIPSEKTPVFPYFRDKKEILNPDPRKNQITIEDLLTMSSLLECDDDNQFSNGNE
                     ERMYLIEDYIQFALDLPIRGFAAWNPKPQDSPFGRSFSYCTAGVILLGGVLERSSNMR
                     VDAFAKKYLFDPLGIQQTAWQITPTGMPMTGGGLGLRSRDLLKIAQLYENKGIWNGKR
                     IISGSWIEKSTKPHAQARENTDYGYLCWLQKFGSNDKTYFGYYMAGNGGSKIAVFPEL
                     HLTVVITSTWYGTGKAHAQSEKILSDYIIPAVQLESK"
     gene            complement(30205..31026)
                     /locus_tag="PK754_00130"
     CDS             complement(30205..31026)
                     /locus_tag="PK754_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002976975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNB07939.1"
                     /translation="MKEMQTGQFFGETDKIMHLDGITLTKTVYTHDYVDWHYHQNAYF
                     TFLLRGGLIETDRKKTVRCAPGDLLFHYWQESHCNIKPPEFTQGFHIEIEQSWFDNLE
                     LDRRMFEGSFQITDPAVRIAFYSMARELGCPDTASVVSIEDSLCRALVHVSKTTAFHS
                     NKPLWVTQIRDVLHARPFDTHTLLGLAMTLHIHPVHLSRAFTQYFGVPLGTYIRQIRL
                     QKSIERMYDPSLSLTNIALQCGFSDQSHFVRCFKAVTGITPLRYRRELCKINEPC"
     gene            complement(31389..31832)
                     /locus_tag="PK754_00135"
     CDS             complement(31389..31832)
                     /locus_tag="PK754_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB07940.1"
                     /translation="MIIETLSEFFERDLLKLKEEIASYSNETAIWKIEGDIRNTAGNL
                     ALHLVGNINHFIGAVLGKTGYIREREREFSDSDVPRIKLIQDIDTTIAMAKTTFSRMD
                     DSVLYQNYPIEKHGKTVTVEHMLLHLLAHLNYHLGQVNYHRRLIG"
     gene            complement(31860..33392)
                     /locus_tag="PK754_00140"
     CDS             complement(31860..33392)
                     /locus_tag="PK754_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB07941.1"
                     /translation="MQNDLEVYIQARYPLIYLVSWEEERILKTLDQIAIHLRKMLFVW
                     TQTQGLYNYVLPDHLDESKSDPEVLLNHIAASQDNGIFVLFDFHAFIENHRIRRQLRD
                     LSKLLRKSNKTIIIVAPQMTIPLELSKDIAVMDFDLPGLTELNIAFQNALKVLHKRRD
                     VVLELTPQISEKLLKAAQGLTLVEFENVLAKSVVHSKAINAHTIQEVLEEKKQIIRKS
                     STLEYFSPDENFEQVGGLENLKQWLTKRAEAFTERAAQFGLPTPKGLLLVGTQGCGKS
                     LTAKAVAAHWNLPLLKLDVGSVFSGIVGSSEANMRHAIKVAESISPCILWIDELEKGF
                     SGMASSNFSDGGTAARVFGSFTTWLQEKKKPVFVIATSNDITLLPPEILRKGRFDEIF
                     FVDLPEQVEREAIFTIHLQKRHRTTDSFDVVKLALLSEGYSGAEIEQAIIAGLYDAFE
                     AGRELTTDDISNNLRATVPLSRVMAEQISALRQWAEKRARKASRTRTAGTGIDLDRFS
                     VN"
     gene            33662..35122
                     /locus_tag="PK754_00145"
     CDS             33662..35122
                     /locus_tag="PK754_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07942.1"
                     /translation="MKRLWTLLLLVFMIACEKEKSDGTLTDDQKEVVEAAVKTTALVS
                     QVDDEFSDNEEITSENGYNGAGDESDYTASSIIKDISDEHLMKSSSSFTFVGRDTITR
                     NYPDGTQATIIRVFDPTTLRLTSIDVATKNFPNSSRVEKKFYRATFTYDPNVIITQNT
                     VMTSSFKDSTIIKTYEEYPGFTQITNGTIIRNVGRVKFADRKLSYSATTTGFATVSGI
                     SNRVTSISIDPVGGTSHKETTTNYTDASSSTSEITYTRTVNATTGERTLSGTMDITLR
                     DGTHRYSVSTMNITADSSNLSKSGTIQIQTDFPAGDSLYRKITKVIMSGAVSTDSSTF
                     YFLDGTTYSTVLTITRTSAPTGNGTPEGLSYTITGSDSRGRTLNGTVTGKRYNFETTG
                     TITESDGSTSQFTLIRDGSSVTLTFTITKTDAALSSTGSFVFDLQNKTGGGTITNGDI
                     TMVYVFTGMCEGYYYPENHENLKQDIEGCMFSLLKL"
     gene            complement(35189..36088)
                     /locus_tag="PK754_00150"
     CDS             complement(35189..36088)
                     /locus_tag="PK754_00150"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNB07943.1"
                     /translation="MNQQITINGVGIHSGKPSRILIRPHEHGVLFNVEGTPIPALIQH
                     VKDTQRATTISVGDRSLRTIEHLCSAIYGLNLSGAEITVTGGEIPILDGSAMPFIEAL
                     ADTLTTESQNEALTIDRTITHRNPDNGAFIRIEPSQNFHVTYTQRFQKRAIDTFSFFS
                     SPKNYIKEIASARTFAYAGELQLLYRKGFIRGLDPNAGFIILDSEPNWDLMEEIIGED
                     ARSAVHETPFYKILSKIPPRYPNEATRHKVLDILGDFSLLGMPIKGLITADGTGHAEN
                     IALVQKIAETYGLIHFDPLGDDE"
     gene            36222..36788
                     /locus_tag="PK754_00155"
     CDS             36222..36788
                     /locus_tag="PK754_00155"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNB07944.1"
                     /translation="MNIVHNSGWIEVIAGSMFSGKSEELIRRMRRAEIAKMRVQVFKP
                     QIDSRYSEDHIVSHSELKMPSEVIEKASEIMEKLDPRTDVVGVDEAQFFDASIVEICN
                     KLAYMGKRVIVAGLDKDYLGRPFEPMPQLLAVAEVVDKPMAICMVCGAPANYTQRLVQ
                     SADRVLVGATGTYEARCRRHFDPPKDTK"
     gene            36815..37780
                     /gene="obgE"
                     /locus_tag="PK754_00160"
     CDS             36815..37780
                     /gene="obgE"
                     /locus_tag="PK754_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNB07945.1"
                     /translation="MFIDFVQIQVRAGHGGDGCVSFRREKFIPKGGPDGGDGGKGGDV
                     VFEADENLHTLQDIVHHRIFRAQHGQKGGSQNMSGRFADDIVIKVPVGTIIKDQETGE
                     MLCDLTENRQRAVLAKGGQGGKGNSRFKSPTNRTPREFTPGERGEEKFLDLELKVLAD
                     VGLVGFPNAGKSTLLASVTAAKPKIANYPFTTLTPNLGIIKIGEYRSLVLADIPGLIE
                     GAHEGKGLGVQFLKHIERTKALAFLIDVQNANPEKDYVTLKKELKAFNKELLNRPRLV
                     VLTKIDTVDTLPKRTKMKDGTPVLMISSVAQTGLEKLVKTLWKML"
     gene            complement(37811..38905)
                     /gene="ychF"
                     /locus_tag="PK754_00165"
     CDS             complement(37811..38905)
                     /gene="ychF"
                     /locus_tag="PK754_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNB07946.1"
                     /translation="MPLRCGIVGLPNVGKSTIFNALTAAGALAANYPFATIEPNVGVV
                     TVPDHRIDALIKVVKPKRVLYTTVNFVDIAGLVKGANAGEGLGNQFLSHIREVDAIGH
                     VVRCFDNADVVHVEGSVNPERDIAIIDTELVLKDLESVNKRISQVERTAKSGDKDAKL
                     AFDVLSKVKVQLNDGKPVRLMSLSENDRKAIADLFLLTDKPVLYICNVSESDVLTENN
                     YVKQVRSIAEKENARVVVISGQIESEIQQLPEEERKGFLESLGLKEPGLHALIRETYS
                     LLDMISFLTAGVEEVRAWTITRGTKNPQAAGVIHSDFEKGFICADVIWWEDFVKHGGE
                     QACKDKGLLRTEGKEYVVKDGDVMHFKFNV"
     gene            complement(39014..39574)
                     /locus_tag="PK754_00170"
     CDS             complement(39014..39574)
                     /locus_tag="PK754_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNB07947.1"
                     /translation="MRKWIIVFLSLACLFFYLKNYDISSVEGRSENHGVIIKISDGDT
                     FLIEVNGKTEKVRLIGIDTPESKDNLKAKKDAARNSADLDAMVRQGIEARRFVERNFP
                     LGTILRIETDRDTRDRYGRMLAYLFTEDDTMINETIIREGYATPLFYSPNYKYKSRLE
                     EAYRYAKSNKKGLWQNGKPLHIRKDD"
     gene            39674..40048
                     /locus_tag="PK754_00175"
     CDS             39674..40048
                     /locus_tag="PK754_00175"
                     /inference="COORDINATES: protein motif:HMM:NF018565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1257 domain-containing protein"
                     /protein_id="HNB07948.1"
                     /translation="MSKFVSLKTALRERVHLENALKEMGCEISNSKKIKTLLGRSYDV
                     DLTAKTAFGMIGFIKNRSGEFEIAGDDMVLASDKDFVGKLTQKYAYSRVVSEAQKAGF
                     QLVKESVNEDQSVRLVLRKWNG"
     gene            40036..40260
                     /locus_tag="PK754_00180"
     CDS             40036..40260
                     /locus_tag="PK754_00180"
                     /inference="COORDINATES: protein motif:HMM:NF022657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2997 domain-containing protein"
                     /protein_id="HNB07949.1"
                     /translation="MERVGGAMTSDYKEIVFTITRDGSLEYAIKGYKGGACEDLSKLF
                     ESMGAVIEEKKTYEYYEKDEDVTQKIRLQS"
     gene            40374..42842
                     /locus_tag="PK754_00185"
     CDS             40374..42842
                     /locus_tag="PK754_00185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNB07950.1"
                     /translation="MIAVKHDKRFFIRNTLIAVGFGLLSIGMSYIRFVIPTFNFMTSS
                     SLNEVAILISILFLPHWGFVIITGAMTAVNAANELQLFSAVSHGFSAAMAWLVYHRFF
                     SRFTKSRQEPILWVMVVILYYYLFLLPQIVGLYLLFDPNPMPAMHITSAFFVNMTPEL
                     FATLLITLLFSIMRQELLSRRETERALAEKEERYRIIAEQTGQLIYDLDVTTGAITWT
                     GAIEINTGFTKEEFLRVDLKTWETMIHPEDRPNAISLLDEAMASGNRYHVEYRFRKKD
                     GGYVFMDDNGLFLRDEKNTVYRMLGTMTNIDERKKAEKSIKENVERFEIIARATNDAV
                     WDWDARQDKVWWNESARKLFAVEEYYPQDNYRLWQERLHPEDRERVTQKYKNVLESTQ
                     NFWSDEFRFRLPDGSYGYFYDRAYILRDEQGRPTRVLGSMTDMTERKKLEQNLIQAQK
                     TESLGRVAGGIAHDINNMLAVILPTAELLLNQNYDKHTVHQHADVIVSSARRASDIVK
                     QLLVFARQTPSSAALLNINNLVDETRNMLDRFLGKNITITLHLEPEIPYIDADATQVQ
                     QIIINLCVNARDAMNNKGSIGIETKMVELDDTESAKYNVPEGLYVQLSIADSGTGIPP
                     EVMDKIFEPFFSTKDIGKGTGLGLAVVKTIITNHGGYIHVHSQIGVGTRMELGFPVSQ
                     RVTIPHVQAQKPQYETGTGTILLVDDEKPILEMGEMVLKRLGYQCYTAENGYRAVEVM
                     NQKPVDLVILDVQMPGMDGIETLEKLLEIKPELKFLFTSGYIGADRLQLLHSKYPDHI
                     VNKPYTVEELSKAIKKLLDTQSAG"
     gene            42953..44320
                     /locus_tag="PK754_00190"
     CDS             42953..44320
                     /locus_tag="PK754_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1446 domain-containing protein"
                     /protein_id="HNB07951.1"
                     /translation="MKDKIFIANGQGFWGDSIDAPVHLVQGGQLDYLTLDYLAEVTMS
                     IMQRQKLKKPELGYATDFVQLIERILPTIVEKNIKVIANAGGVNPEACRQALFAVAKK
                     LGIKGLKIGVVYGDNILPRISEMETKQIPMKSMDTGESLYDLMKQGRDILSANVYMSS
                     LPLVEALQKGAQIVVAGRTTDTGLAMAPMIYEFGWSFDDWNKLAAGTVAGHIIECGAQ
                     CTGGNFTRWQDVPDMWNIGYPVVEAYPDGTFYVSKHENTGGMVTVDTVSEQLVYEMGD
                     PHNYITPDVIADFTSIRLEQAGKDRVKVFGVQGKPSTEFYKVSASYLKGYKATGQLTI
                     SGPDALPKAKLAAEVLWKRLTRAGMEYEETHTEFLGVNTCHEGMVAIPNPINEVVLRV
                     GVKDKDQKKVDRFGKEIAPLVTSGPPGVTGFAGGRPKPQEIVAFFPCLIPKHLIETTV
                     SVEEV"
     gene            44342..46096
                     /locus_tag="PK754_00195"
     CDS             44342..46096
                     /locus_tag="PK754_00195"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB07952.1"
                     /translation="MKKILYIEDDPVAMTYMTQLIQRMGHTIITATTGESGLAKAFAE
                     RPDLILIDLLLPGLDGFELTIKMRSSETLKHTPIIATTATPNEDDQQLATMAGCDGFL
                     VKPIEYEALSDLIRGCFDSETHTNKNVSLESENIELRRFTLRLVHRLTDKINEISYAN
                     KELQKTNNTLDRLIQEVRTNNADLMQINQLTSQIMAYRDRSTIYREMPQLISDQLSLA
                     GAAIYVVNEKDLTLDVFSSYKYDFLPETEHIPFTEPPFFETVYDNQSVTIDVHKLIAL
                     QRENERHARRLEVLFESFHAQTVCFVPIRGRARAADDFECENQDCEAFVKKDGQWWRN
                     LIHQYNRRDLDYPSRLRDASRYFFNCCFYDLKGVLALGIPDGRMSDAFRQIIHSFTTT
                     VGLRLDNVQLYEDVREAYMLAEKQAITDAMTDVFNYRYFHHQLEREIRRAKRHWSKLS
                     MIMIDVDHFKSYNDRHGHPAGDEILKRLAVVFKNITRTSDIIARYGGEEFVIVLPETP
                     KVAAVKFAEKLRAAIQDEDFPYEQTQPMGDITVSMGVATFPDEAQTIDELIQRADEQL
                     YRAKSGGRNRVAYSNTTG"
     gene            46101..47732
                     /gene="bshC"
                     /locus_tag="PK754_00200"
     CDS             46101..47732
                     /gene="bshC"
                     /locus_tag="PK754_00200"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HNB07953.1"
                     /translation="MTISFRELKNTTSLFTDYLYSPEKTTSFFSANPSDQKAWSDTFS
                     EVLKKYKTNRELLSDILADQQHRFQSAKQALENVALLRQPNTVAVVTGQQTGLLGGPI
                     YTVLKALGTVKYCRLLKTKFPDYNFIPVFWLELEDHDFEEVRSTQLINIQNELVKIFY
                     DGGISENQNKTPVHRFALTDDISRIIQELKTALNPTDFSQALFDTITKSYQPGKSWAE
                     AFGMLLSSWLSPMGLVLMDPSDVRFKGIVKPVFERALKESESLHEAVLNQSNALKVAG
                     YHAQVETEPSYVFLTNYGENKLSFNNKGHDKIRITRDIWKTYKNDILHILENEPQRFI
                     PNVILRPLIQDTLLPTVFYVAGPGEIAYFAQFRDLYTHMDMASPVIIPRPFFTLVEKK
                     IKKVLDKYQITIADIINDGEAVIERINQQSNVSLEPALLRFMNEVQDGMKNLETQLTA
                     IDASLKGASATAMDKMQQAMQVLTQKTRDAEKRAALTTHGQLTKAMLHIRPNDNFQER
                     ELSILYYLNKYGFTLFDTISEQITLDSWDHRLIEL"
     gene            47884..49704
                     /locus_tag="PK754_00205"
     CDS             47884..49704
                     /locus_tag="PK754_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HNB07954.1"
                     /translation="MDNGSPRNLADVFHQSARRFGHKTLYMSKRDGKYRALTYGQVHE
                     IVCNLALGMASLGIKKDDKVAILCPTQEEWAMSDFAILSLGAVTVPIYPNLPTPQVEY
                     ILQNSDARLVVASDREQLAKIIECKNQCPMLNNAVLITHEDKHKDYVLFFSELQELGK
                     KFGTENPGYIEKQIDSIDPKGLATIIYTSGTTGMPKGVMLSHNNILSNSLAAVKVATV
                     GETDTMLSFLPLAHSFERMAGHFMPVIRGCAIAYAESIEKVPQNMGEINPTVMTAVPR
                     LYEKMYDRVKEGLSTAPPIRRKIFEWALGVGKSAYETGERGFKYKIAEKLVFSKLHHR
                     LGGRLRFFVSGGAPLAKEIAEFFAYMGVMILEGYGLTETSPVITVNRPEKVKFGAVGP
                     AIDGVEVRIAQDGEVLTRGPHVMMGYYKNEEATREMIDSDGWLHTGDIGFLDSDNHLH
                     ITDRKKNIIVTSGGKNIAPQPIENTLITIPFVEQALVIGDRRNFISALIVPKRERLRD
                     WAMQHGIKFDSEEELLENKEVYAMIDKLIQEAQDKAGFARYEKVRKFALLPKAFSIES
                     GELTPKLSIKRHVVTKNFKDMIDTIYNRETERMTEGDSLT"
     gene            49701..>50753
                     /locus_tag="PK754_00210"
     CDS             49701..>50753
                     /locus_tag="PK754_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07955.1"
                     /translation="MIDIKHILTVGVDDQVKTIYEDYIAQLTERIGISDANAKPAFKA
                     LMNILITLEESGNIRKGYDSLPSLFITQRVLYMKVFDLMLQHGHAVSDVDEFWFRGKE
                     IELASPSHFIDDQVQVARSIFSVFQPFLDADDDVDRFMRVISLYAAFISKKNDVGAMV
                     NLMHHFAHFADKIYLAGYKKVHDGWGGKLDLDTDVYPVFFKSIIRDAIETYGFSDYVV
                     DFLYDRLKEVGESETVLPDLYDFTKSLQANAELTKEIQDADKSLKTEIQKTLQIVQQQ
                     KGESLSENAQNAIVRELTAYEELEGLIYYYFDERRLFKIMTDTKTVSLFEKTLDIVRS
                     YSPKMKHYVMYAFGKLL"
BASE COUNT        15169 a         9938 c        10512 g        15134 t
ORIGIN      
        1 ttggcttctt tggcatcggt aaccaagata tcgttattcg gtaaaaactt aggatctgat
       61 gtgttgtgac ttgcattaaa gctcaatgga agagaaatac cccatctttc aatatgaaat
      121 ttatgaaggt ttaggttccc actgatactc catgattttt ggtttcctcc ggatggatta
      181 aaacgttgtt cgacagttcg aaaatcagca gaacgatatt ccgtacttcc actgagagtc
      241 agcatatctc ccacatttag tgataagcga gccatgacgg cctctccgga tttatcatcg
      301 gcatccgtta cgcgaagctc gttaaaccat atttcaccag tataagcgtt tactaaagat
      361 gtgttgtgca cacctaaata aatgaactga acattgtcca aagatggatt acctttgatt
      421 cgaataatct tgttatcgcc gatattgtat tctgtaagag tatcgttgcc ggttctctga
      481 aattttaagg aagtaaggtt ttctcttata tcaatatcaa gtgagttttt ttcttctgac
      541 catccggcat acagaggttg ttcaatttcg taataactat tgaatcgatc agcaaagcgg
      601 ataaaatatt ttagaggact atcactactt tcatctgaaa tcggcaaagg tatcgaaccg
      661 cctttatccc cgtgaacaaa cattctaatt cgcccgtagt ttcgaaaatc tctttgcgag
      721 ttggatttgc taattattgc ttttgagtta actggtaatt cgttcagacg aaggagtaat
      781 gattgttctt ttacttctct ttgagcgtta ttcccatttt gatcaattgg tttttctctt
      841 tttactccgt ttggtcttgt ataaccaccg ccttcatcag tactaataga ggaaatttct
      901 actattttac tgttttcgtt atttgatgga atttttccat tgggatttag taatacccca
      961 ttgggtacct ctatgggcaa tttccactca ctattgataa attgaatttc agataactgt
     1021 acgctaatta tttcagactc tggatcttca gaggaaatcc aaattcttgc tgaagttatt
     1081 tttggaagta caaggtcgat tgaatttgaa ttgataatcg tgtctggcct taaaataggt
     1141 aaccgataga gaatccaacc gtcttcgccg cgtccaacta taatatcatt atctaagcta
     1201 tgtgtatctg ttgtaacact gtaccgagca taaacgtttt gtatgtcaat aacaccgttg
     1261 ttattgatat cttcagtatc ccctattctt aaaccaccat ctgtaatagg aaaagtatta
     1321 ttttcgtagc catttacttt gatatttctt atgtcccgat tagcttcggc gttttggata
     1381 tctttgtttc caaaacgggc ttgttcatta ttccaattat caccaaacgg atcatcagga
     1441 tccaaagcga ttgttcccca tggaaaaata atttgtaaac tatcaactat tgctttttgc
     1501 gaaaaactca ggtttccata ttgagaccta gtaataccta atttggataa aaggctatct
     1561 tcgccggcat cacttagatc atcaagccct atatcttgat ttttttcaat tacgggctgc
     1621 aaagctgatt tgacctctcg attaggagct cccccgtttc cattttgatc ctcagaaatt
     1681 tcaccaatct cgatatttat tttagcgtgt ttaccctctg gagtgttatt ttttacccaa
     1741 aattcgataa aacggcttgc tccaagttct ccagctgatg acaacgaaaa accacgcatc
     1801 actccacccc ataggccttc cgttggggcc tcatcactta ttttattttg taaggttaga
     1861 gttaatacgg tcgctctatc tgtattgtta gttttaatat tggatatttt gcttcttgaa
     1921 atttcatttt gataccagat taattttcga cgcttggaag cgtttttctc aataggtttg
     1981 ctggcaagag tccaggattt tctattcgaa cccatgggat aggttctttt tattgcttca
     2041 aaatcatcta tatacgcaac accattttca tcattttcca tcaacccgtt acttgtatta
     2101 ggagtaggaa gcagttttgc gtattcagct gcaaaattga tttttgagtt ctgattggat
     2161 gaaacaaaag gaatatagtt aatgaatttt gttaggtaat tagcttctaa ttcaatattg
     2221 gtattaatat cccatacaaa gttattaaaa ggctcttcat tgatttgaac gcgtttgttt
     2281 actgtagatt gactatgaaa aagcgccgtt gtcccaatga atgaatttga gccgaatccg
     2341 taatttgcca aattatattc tgcgcgggta ccaaaaatgg attttttatc aacttgaaat
     2401 aactgagcct gctcatactc tatcttaata ttggctgttg ataatgctgc acgcggatca
     2461 attaatatca actctcctga ggtatagtca atggtatagc caatgttttt ttctaataca
     2521 tcgccatcta atcgaacaac ttcactgctt tctaaaacat taatacctaa agagaatttt
     2581 gcagagctac tttttttgct ttctatttct atcgaatagc gatcgttaaa ttgtttaaat
     2641 ttgtctgatg atcgagcttc attatatatt ataggtattc gtacttcgcc gagattggga
     2701 tcattattta atgcgggaaa aacctcgtca cgaaaacccg gaagcggtgt agtgggtggg
     2761 tcaaaaggcc gcaagcgtgg aaaaataatc caaccattat cataatctaa accatatccc
     2821 atcaaaactg tcgatgaagg agcgatatca attatttgat ctggatcttt tgatttaccg
     2881 ttttggctag tttcatccac gtgaagaata tcaagtaatc cccggtcttt gccaccaata
     2941 tttgggaaac tagttatatc attattggat gatggtttaa attttatttt tacactttct
     3001 ccttcttcgg cactcaagcc ggttataccg tcaaaagcgt aaatattttt taactctaaa
     3061 tcccaagttg aatctgtagg aagaggggtg ggcttccata agagttttaa tttcaacgtg
     3121 tctgatgaaa catctccaaa tacctgttga gatgtttcgt aataaacagc taatgcctca
     3181 ccaacagtga gttttgtact gaggtttatt attccaagct ggaaattaca agaaaactgt
     3241 tcttttggaa tttctaaaaa aatcatagtg gccgcatttg cagaatccaa atcggttatt
     3301 tgtgaaactg tcatgttagg cagatttgga tttgtcaaag ttgaattatt ataggttaca
     3361 ataccgatcc ttttttcagg gtttctatcg ttgccctcac ttttgcggaa aagccttata
     3421 ttgataattt catccgcagg atcgtaagtg tgctttcgcc cgtcttgggg atcatacatt
     3481 tcgtaacgat ttaaataccg tttgtctaaa aagaaaaact ttcctttttc ataatcttta
     3541 gctgattttt ttattattga ggtcgaatta ttgcccgaac cgggattgat ttcaatcact
     3601 ttcttttcgc cgttttgtat acttgcgata gatgtaagtg taagatctcc aaatttaaag
     3661 atagatttta ctccaaaaag acctttgttc tgaccaccct gtgtcacgta ttgtgttccg
     3721 ggtaaactta aatctatatt gccggcctct aatttttgta aaatttcatc atcaaaaccc
     3781 gtataagtta tttttatatt attttcaaaa ctaaattcac gctcactatc ttgatcaaat
     3841 aagatctcaa ctttttttcc tattgtacca cgtaagttga atttttgttg ttgttcgaat
     3901 tttggtatga attgagaact ttttccggta gatgagcttt ggttagaaga tttttcgctg
     3961 gctcctgaaa cattcaaatt tatatttcct gtagcatcta cggtgacttc atcaccacca
     4021 aatatttttt gaaaggtttc gttggttgta acgctggcac taaagagtct tacggccccg
     4081 gattgacgtt cttcttttga agcaatacca gtaaccacag ttttagtaaa aacacttcta
     4141 atctgataaa caaacgaatt tgatatataa gattcctgtt ttattactgt tgtttcttta
     4201 tatcgttgtt tttttgttgt ttggccaaat tcgtcagcaa aaacataatt gcccgatgaa
     4261 tccaacattg ctattacctt atatcctggt aacaactttt gtgtttgaga aaaatctata
     4321 gaaccaccac gtattaaaaa gtagttttga ttggggtcta tagaaatcgt gttatgatat
     4381 tgaaatgggt ataaagaaaa taacgatcgt gttgacctag agtctttaat tttttttgcc
     4441 tcattttcaa ttacttgatt tatcactaga tcaagttgac cggttattga aagattggcg
     4501 ttaatttctg acattaagct tattaattct gtctccaaag cttttattgc attgagatca
     4561 ctccaaacag aatcttgcct aacatcctta gacgtagggg ttttattaat gtttttttca
     4621 atatcttttt cgttaatgaa aaataaaatt gaatcagcgg tttggagtga aacggaaata
     4681 ctatctaaat cctgagaaaa agatttagac ggattgagaa aaaaaagtag aaatgaaact
     4741 acgagaaatc tttttgagat aatatggaga aacaatccac ccacctgcag atcatattga
     4801 tgaatcaaca atcgcacaat caaagcctta tttcggataa gttcgtctta agctgaaatc
     4861 aattcgtagt aaaaaaggcc tctcggatga tctccctcgg ttggattata atttaataac
     4921 ttaaaaaaaa acgaactacc tatcaagaaa aaattttatg tcaactaaag cgctttcttg
     4981 atcactagga ataaaaggga tgttttgaaa gcgatgaatc gtcagtgtgt atttatctgg
     5041 ccaatttgat tttttaaccg attcaacttc tatatctacc tgcttccctt ttaaaaaaag
     5101 catccttccc tcgtttttta aaaacggact cgtcaaagga ataagttcat ctaaggctga
     5161 aactgcacgt gcggttacga attcataatg agatgaaaaa gattttgttt tcgcaatttt
     5221 ttcagctcgt tcatttatta ccactaaatt tttcagaccg acgacctttg aggtttcttt
     5281 aagaaaatca cttttttttt taattgattc aactagtgtg aaatgtgttt caggaaaaat
     5341 aatatttaaa ataattgcag gaaaacctcc accactgccg atatctatca ctttttgttc
     5401 ttttttaaac ttaacaaaaa aagcgattaa tgcagaattc agaaaatgac gagaaataat
     5461 attcccctct tgacggccga tgagattaat ttttttattc caatctagaa gaaaatcatg
     5521 aaaagaaaaa aattgatttt tctgaatact gcttaattta agtgtattat tttcaagtat
     5581 ttgaaaaaaa acatcccaat ttatttgatt gttttgcata tattaattta gtgtgtcatt
     5641 aaatgaatct tgtatattct ttcctaaaga acctctttcc aaaaaaacta acaatagcga
     5701 tatatcactt ggagaaacac cggaaattct actcgcttgc cctacgttgc ctggtttaac
     5761 tttttttaat ttctcacgcg cttctttcga aagtgatgtt attttatcat aatcaaaatt
     5821 gagtggtatt tttttgttct ccattcgcaa aaatctatca acctgctcct gttgtctttt
     5881 taaataacca tcatacttta ttgaagtatc tacttccctt aaagtgtctc gatattttaa
     5941 taaattaact atttttggtt cgaaaatttc ttcttcacac tctaataaat cagttaaagt
     6001 tacttccggt ctttttagta ttttagccag gggttccggt tcttctaaga ttgtggaccc
     6061 cttatttatt aagaaagagt tgattttttt tgggtgtaaa ttagtttttt ctatccaagt
     6121 ttctatttga tttattgcat ccttttttct gataaacttt tccatttgct tttcatcgat
     6181 aaggccaaag gcatttcctt tttccataag gcgcatatca gcattatcct gccttaggag
     6241 taaacgatac tctgctaggg aagtaaaaat gcggtaaggt tcaagggtgc ttttgttaac
     6301 aagatcatca ataagtactc cgatatatgc ttcgtttcga cgtaaaataa atggatcttt
     6361 ttgctttact tttaaagccg catttattcc ggccatcatc ccttgagcgg ctgcctcctc
     6421 atatcctgat gtgccgttga tttgtcctgc aaaaaagagc ccttctacat attttgtctc
     6481 catattcaaa ttaatttgat acgggggaaa gaaatcgtat tcaacggcat aacccggcct
     6541 taaaacatgt gcgttttcta atccagttac agatcttatt gcgctgattt gaatttcttc
     6601 aggaagactt gtagaaaaac catttacata gtattctata gaatcataac cttccggttc
     6661 taaaaatatt tgatgtcttt ctttatctga aaagcgattt attttatctt ctattgatgg
     6721 gcaatacctt gggcccaacc cttttattct acctgtgaac attggcgatt gatcaaaacc
     6781 tgatttcaaa atatcatgag ttttgtcgtt agtataagta agatgacaag gaatctgagt
     6841 attggttata ctatctgttc gatatgaaaa cggttctggt ggattatcac catattgtgg
     6901 ttcgcaaacc gaaaagttaa tactatcttt gtgaattctt ggaggagttc ctgtttttaa
     6961 gcgagaactt tcaaatccta aactaattaa agactcagtt ataccaagcg atggtttttc
     7021 tcctattctt cctgctctaa cattttttag tccaatgtga attataccgt ttaagaaggt
     7081 accacaggta agtattacag ttttaccttt aataacctca tttttatcag taattacccc
     7141 tttaatttga tttgactcaa ctacaacacc gacaatattt gcatcgcgaa tggatatatt
     7201 ttttttcaaa ccaagaagct cttggatttt taatggatat gcagctcgat caacttgagc
     7261 tctaggtgac cacacagcag ggccgcgaga cttattaagc attctgaact gaattcctac
     7321 ctgatcagtt atgaacccca tttcaccgcc aagcgaatca atctctttta ctaaatgacc
     7381 ttttgctgtg ccaccgattg ccgggttaca agataatgtt cctattttag tggtttccat
     7441 actaagaaga agtgtactgc atccaagtct agatgctgca aaagcagctt catagcctgc
     7501 atgcccccca cctactatta ctacatcaaa aacatccatc tgctttttat tgtttcacgt
     7561 gaaacagttt gatagttaaa atttatgaaa tattagtttt ttactttttg tttcacgtga
     7621 aacatgctcc tatttattat aaacatgttt tatttaaaaa gtaattttct gtttttagga
     7681 gcacgagtta attcactttc caatacaaaa atttgaaaaa atgttgttta aaacatcgtc
     7741 tccagtaact tctccaatta tctctccaag gtgacgaata gcgtttttaa tgtctagaga
     7801 aatgaactca taagacatct tgttatgtag gctaactttt gcgttattta atgatactac
     7861 gctgttttct aaagcatctt tgtgcctcaa ccgagttatc ataggtgatt caatagagaa
     7921 attgttaaga aataagtcgc ctatacttct ttttagttta tcaatcccta tatcgttttt
     7981 tgctgaaatc tctataaaat cgtttttggt tctttgtgga ttatagtttt ctttgtcgca
     8041 tttattggca atgaataagg cttttacatg cttttgtttc catattagat cttcaaccat
     8101 ttttaaaata ttaatatctt cttcgtttaa atcttctgat ccatcgaaaa taactaatat
     8161 caagtcgctg ttttttgcta tctccattga tgattggatg ccttgatttt ctactacatc
     8221 tttagattga cgaataccag ctgtatcaac aaatataaaa ggtattccct ctatatctaa
     8281 actttcttca ataaaatctc tcgtggttcc cggtatgtcg cttacaatag ccctatttcg
     8341 ctttagcatt gcgttcaaaa ggcttgactt cccgacattc ggttttccta tgattgatat
     8401 ttttgcacct tctcttataa ggtggcccgt gttgtatgta gataagagtt tttctatttg
     8461 tagacgtaca ttggttattt gcgcatcaat ggattctttg gtaatattga ttagattttc
     8521 ttctccaaaa tcgagatcta attcgagaag cgaacaaagc ttgataagtt gtttttttaa
     8581 ttcgtcgatc tcatgagata agtttcccat gaattgtgcc atggacgaac gtctcgcgct
     8641 ttctgttttg gcgtgaataa gatcggccac cgcctcgact tgtgataaat ccatttttcc
     8701 attaagaaag gcaattttag taaactctcc tggctcagca tgacgtgcac catacttgat
     8761 acagacatct aaaatccttt tagcgtttaa taccccaccg tgaccatata tttctatcgt
     8821 atcaagacct gtataagttt tcttaccaag cataaccccg atcaagacat gatcaatctt
     8881 ttcttttgtt aaggggtcaa taatataacc gttatgaagt gaatggctta tatgttggct
     8941 tggttttgaa tgacctaaaa aaatttgatc tgctaaatta acggcttctt taccgcaaac
     9001 gcgtaccata caaacccctc ctgttccgat aggggtactt atagccgcaa tcgtttcgtc
     9061 aaaattcatt tttggaaata attaaaataa aaagtcactc ttcaaaccta tagttatccg
     9121 agtgacttta aaattagatt atgttatttt acttcaacaa atttttgcat cattacttgt
     9181 tgcgccatcg tcaatacgtt gaatactgtc cagtataaaa ccaatccaga tgcgaatcca
     9241 ttaaaaagga aaaagaacat aatcggcata ccatacataa ggaatttttg attaaaacgt
     9301 tgagccggat cagagtcgtc ccccatcggt gtcatcggtg caattttttg ttgtaaaaat
     9361 gtaagcaccg tcataatgat aggaaggggg tttacgtgga aatcgccgat aaaaggtaag
     9421 cctgtatgta atatagttat cgtatccggc cttgataaat cggttatcca tccaaaaaaa
     9481 ggagccaagc gcaactctat gctttggtta aaaacttgat atattggaaa caataaaggc
     9541 atttgtatta acatagggag gcaacctgaa agaggattgt agccggcttc tttgtaaagt
     9601 ttcatcattt cttgttgcat tctagaagga ttatccttgt acttcttttg aatctctttt
     9661 tgccgcggca taacaacttt cattttttgc atgcctacga aagtcttata agtcaaagga
     9721 taaagaacta ttctgactaa aagagtaaaa actatgataa cccatccata atttgggatt
     9781 atgtcataaa gaaagttgaa agtataaaaa atagctttcg taagtggcct aaatacggcc
     9841 caaccccatt ccatggttac agctacatta tcatcatatt cttttaaaat atgataatcc
     9901 atcgggccaa tataaaattt gtaacgatca acaacattcg gttcaaactt catttgaata
     9961 ttcatgaagt ttgatttttt tgtaagtaaa ccattttcgt gagttgttgt gcttgttagt
    10021 ttacaaccaa tcccatcttt ttcttgtgga ataatatata cagcaaagta ttttgttcga
    10081 gaagcaaccc atttcgtaac tccggaccag gtggtttctt tggagggatc agttccaata
    10141 tcaaattctt ctttttcatc gcccatataa gcataagata tggtatgact tagatctccg
    10201 ttgcgatcaa gttcagtgct cttgagtgct gacaaccatt gtatttggta ttcaccatta
    10261 gttatatcgt cgccaaaatt tttaaactga acttctatat caacatcgta tctatcatta
    10321 taaaaagtta gattttgaat gatctgtctt tcggaatcaa aatgataaac aaacgttaaa
    10381 gtgttttttt gtttggttat taaatcaata actgaatccg tgttaataat ggagtatggt
    10441 atattccacg tattatagtt tttcccttga aaaacaaaat tcaggtttag attttttgat
    10501 gattcaataa aatctacagg ttgtccatca tgagttttgt aaggaagtat tttccagctc
    10561 ttaatggatc cgccaccgag atttgatatt tcagctgaat atatttctga attaatacga
    10621 attatttttt cagattgttt tattgtatct tttgtcaagc taaccgtttt aggtagatcg
    10681 ctttcttttg aaatgttggt tttggactgc aaaagagtat caggatgagt tggagttgag
    10741 gtgctatcaa cagctaatgt ggttttaaca gagggatctg caggtggcgc gacagcctgc
    10801 atataaaagg gatacaagat tatgataagg gcaatcataa aaagcccgaa aatcgtacgt
    10861 ctatccattg agtttgtctc cagaaaatat cagcagcatt tattattagt attttgattt
    10921 tgtggcacgg gatcatatcc accttcatga aatgggtggc atttagatat gcgtcggatc
    10981 gtcagccaaa ggcctttgaa caaaccgtga gattttaaag attcgatgct atattgagaa
    11041 catgaaggat aaaaacgaca agaggacgga aaataaacag aaagcgtatg ttggtaaata
    11101 cggatcaggc tgacaaaaaa ataaagtaca gaacgtcgga gcattgtcat accatagaat
    11161 aaataaggct tctctcgtat tttgaaagaa gccttattaa atcttaattt caaaaaaaat
    11221 tattcgtcgc ttactgaaag ttttttacga cctttggctc ggcgggatgc caaaacattt
    11281 tttccgcctt ttgttttcat gcgcgagcga aatccgtgtt tgtttttacg tttacgcttg
    11341 ctgggttgat atgtacgttt cacgtgatcc tcgcagatta tttttagtta aacaataatt
    11401 ttcgttttcc atttactgtg ttatataggt atgtcactct ataagtagca atcagtaaca
    11461 atctaataca cgtatactta caaatgggtg gggaatatag aacataaggt atggaaagtc
    11521 aagcttaaaa ttgttgtttt taataaataa acagttttca tatttaatcc acgctaatat
    11581 ttgaatctat tagttttata aaattctgct gttgacagca tacttgtcaa gaaaaaaaag
    11641 tataaaaaaa atttagaaat aaacaccaaa ataacaaaaa gataactatc cacatatact
    11701 atgctttgac aaagcacttg acaaagacct tgtctttgat aaattgtaat cgtgttccat
    11761 tgtgtttgtt ttgtgttgat aatttgttta tatattcatt attagtgttg ttgttttcat
    11821 ttatttacta attatcccct cgtttttctt aattttttta ttcaaacagt tgtaaataat
    11881 tgatttgatt ttttttgatt tgatctttat ttgttttttt aacctgtgtt gatttttttg
    11941 tacacgtgtt tgtattgtta ataacttgtt tttaagtcat ggttgagcct actacaacag
    12001 aatcgggtaa tatgtcagcg ataatcgaac ttgatgccaa atcggtatgg aatcgctgtt
    12061 gtgatataat caaatcacaa atacccggcc catcttttag aacatggatt aacccaatag
    12121 agcctcatgt tatagaaaac gagaagtttg ttgcatttat acctaatcaa ttttttgtag
    12181 aatggttaga aagtcgttat aacgatttga ttttgaatac attacgcgaa gtttgtggtc
    12241 gttcaatcgg ggtaacctac atcgtaaaag aaattacaga accgatccaa acagtttcta
    12301 cgccatccac cccgctcgtt cttcaagctt ctgtaaacaa acctaaagaa acagccccag
    12361 tagtttatga cagctttatc aacatacgat atacttttga taactatatc gaaggaaatg
    12421 gaaacaaatt tgcaaaagca gccgctttag ctgtttctga aaaaccgggt aaaacgtcat
    12481 ttaatccact cgttatttat ggtggtgccg gtttgggaaa aacgcacctt attcaagcaa
    12541 taggtaatca ggtaaaaaga aacttccctg aaaaaaaaat atgttatgtg acgagtgaga
    12601 cttttacgaa tgattttgtt gctgcgattc gaaaaaatga tgttttagag tttagccgtt
    12661 tataccgtag cttcgatctg ctcatggtgg atgatataca attcttcgcc gataaaggga
    12721 aaatccagga agaatttttt catacattta atacgcttca ccaactgggt aaacagatta
    12781 ttctaacatc ggatcgtccg cccaaagagt tgaaacaagt tgaagagcgt ctgatttctc
    12841 gatttcaaat gggactggta acggatattc agccgccgga tcttgaaacc cgtattgcta
    12901 ttctccaaaa acgcagcgaa gaagacggtg tatatttacc tccggatgta tgcgaatttg
    12961 tcgcaatgaa tattacgtca aatattcgcg agctcgaagg ttctttgatc agattgcttg
    13021 cttatgcctc gataaccaat acggagctca atcttgatgt tgctaaggct gtgcttaaag
    13081 ataccatagc cagcaaaaga aaaaatttat ccatcgaaac cattcagaag atcgtatcgg
    13141 attattattc gattcccgta gatcaaatgc ttacgaaggg aagaaaaaag gaaatagccg
    13201 aagttcgcca aatatgcatg tatttggtaa aagaaatctt gggaagctct ttaaaaacta
    13261 ttggaatcca ttttggagga agggatcaca gcaccgtgat atacgcttgc aaaatggtag
    13321 aagaatggat gaaatcaaac acaaaaataa aggaagacgt tgagatcata aaaagaaaaa
    13381 ttgaactggc ggcgttttaa taaaactcct cctttcacct tcacatagac gatcaggggc
    13441 ttgggatcct tcactcaagc ccctcttttt tatacctttt caatcaataa cgctaaccct
    13501 aagcctccgc taatacataa ggtcgcaatt cccaattttg ctttacgatc atgaagcgaa
    13561 tgaactagtg ttgttgtaat acgagctccc gtacaacccg tcgggtgtcc caaagcaatg
    13621 gcccctccat gtacattaac tttatcttta tcaaaatgta attcacggtc acaagcaagt
    13681 gcttgggcag caaacgcctc gttgagctca ataagatcta tatcttgaag ccgtaagttt
    13741 gattttttta gaagtttttg tacagcgggt accggcgcaa tacccatttc agaaggattg
    13801 acacctgctg atgcataacc gataatacgg gcaaggggtt tcagattatt attttgtaca
    13861 aattcttttc cagctattat caaggctgat gcgccatccg taactccgga cgaagagccg
    13921 gcggtgatcg taccatcttt tttaaaaacc ggttttaatt ttttgaaatc ttcaatagtg
    13981 gcgccaatcc gtggatgctc atctgtcgtt atgatctgat cacctttttt ggtagccact
    14041 tttacaggaa tgatttcatt attaaattta ccggttttga tagcactctc tgctttcaat
    14101 tgggattgta atgcaaaagc atcctgttca tcgcgagtaa tattatattt tagagccagg
    14161 tcttcgactg tagcacccat gagtttatca gccatcggac aaaaatatcc atccgtatga
    14221 ttagcatcca aaatttcact attacccata cgatatcccc aacgtgcttg ggtaagtaaa
    14281 tatggaatat tactcatgct ctctacacct ccggcaagta cgacatcgtt gtcaccgaga
    14341 actatggagg tgtaggcatt gataatggat ttgagccctg agccacaagc tttattaatc
    14401 gtatatgcag tcttttgtga tggaatacca acaccgacag caatttgtct tgagatgttg
    14461 ggtccgatac cggcctgtcg cgcgcatcca aatatagctt catctacaga gtctggagtt
    14521 aatcctgctt tcattattga ttctgctgcg gcgattattc caagttcttt tgctttgata
    14581 gaagaaaaca caccgccgta ttttccaata ggagttcgga cagcactaag aataaaaact
    14641 tctttcacaa aattgtcctt tgttttgtat attctttttt tcaaatttaa aatgttccaa
    14701 acgatactca acacaattct tgcctaaata cgtctgccta aaacactaac cgatcaaaac
    14761 tatgaaaaca cacactacgg tttttacttc tgcggatgaa ttggcagaaa aaacggctga
    14821 aaaaataata tccataggtc atcaagccat atccatgcat ggacattttt catggtcttt
    14881 agctggtggc aaaacaccca aaatggttta tgaagttttg gcaaaaaaac acaaggaatc
    14941 tatagattgg aaaaaagtac acctcttttg gggtgacgag cgcgatgtta tttctacaca
    15001 tccagatagc aactttggaa tggcaaaaaa tgtattgatg gacccaatag ggtttcctga
    15061 tgccaatatt catcgtattg gtacagaaga agactgcatg ttagccgcac agcaagctga
    15121 agatgacatt aaagattttt ttgggttgtc ggatggaggt tttccaaaat ttgatctgat
    15181 acttcttggc cttggagaag acggtcatac cgcgtcgctt tttccaggaa cggatggaat
    15241 taccgaaaca aagtttcttg taatggataa ttttgtaaaa aaattagggc gacatagggt
    15301 aacttttaca tttcctacca tcaatcatgc ttcgaatgtt ttgtttcttg tttcgggtaa
    15361 atcaaaatca tctattatta aacaagtact tcaagggagt acagaagata tactgccggc
    15421 tcaaatggta agacccgccg gtgaactcta ttggtatctt gataaagaag ccgcatccga
    15481 attgcaatga tacccgatta tatatcgcat tcagaaaaag aaacttttga ttatgctgaa
    15541 gattttgccg aaaaaataaa acccggcgat attatagcac ttttcggtta ccttggcagc
    15601 gggaaaacga tatttgcgaa aggtctatgt aaaggtttag gtgttcagga gcccgtgcat
    15661 agtccgacat ttgtaatagt aaacgaatac gaggggtata aaaacggaaa agctcttatc
    15721 ataagacact gtgattttta tcgcataaat acagaaaaag atattgccga attgggcata
    15781 gaggaaatga tggatcaaga agattatgta gtaattgctg aatggtctga acgtgttgaa
    15841 aaaaaactac ctcaatcatc gtggcgagtc tattttgaaa agagaaatga acaagaaagg
    15901 caaattagta ttcgacgcga attatgaaat tattagtatt agatgcatca acatcaatat
    15961 gcggcgttgc gctagtagaa ggtaacgaat cagagattaa gcttatctca agtattcatc
    16021 tcaatatcaa taacgcacat tcagagcgaa tgatgttact tgtagaatac ataatgcaac
    16081 aaagttcgat tagttcaaaa gatattcaag ccattgccgt cacaataggt ccgggatcat
    16141 ttacaggctt aaggatcggt ttatctgtag caaaaggatt agctttttct tttaaagtac
    16201 cgttggtaac cgtacctaca cttgaagcca tcgcgctgcc gtggatagat cattatcggc
    16261 gtatatgggt tgctctgact tcgagaaaaa aagaatatta tatcgcattt tttgaagacc
    16321 ataatgtttt gataaaggga caagtatgga agtttgatga actcgaaaat aatttgctcc
    16381 aaggagattt acttctaact gatgatccaa tatcatttag tgaattatca ggtattcata
    16441 tttctaattc tcaagacggt ttacccaatg tgttttccgt cggtaaaatc ggcttaacta
    16501 aagctatagc gggtatatat gctgatttgg ataatgtggt tccggattat tttcaaggat
    16561 ttcgcggagc ttcatgaact ttaatgagtt gataattccc ggaaatttaa tattgcgcaa
    16621 gatgacaaac gaagaccttg atacggtcat gcgaattgaa ggcgcatcat ttgacagccc
    16681 gtggacgcga agtaattttg cagatgaatt aaaaaatacc gatattgcgt tgccgttggt
    16741 tttggaacaa aacaaattga taatcggttt cattgttctt tggataatat tggatgagtg
    16801 ccatctggct aatatcgctg ttacaccgga ttatagagga cgcgggttgg gtaaacttat
    16861 gatgcaaaac atcatctcta tcgcgcgtca aaacaaatgt gtgaaggtta tgttagaggt
    16921 cagaaagtcc aatacgacgg cgatcgcatt gtatcaaaaa tttctatttg aaaaagtagg
    16981 tgttagaaaa aactattatc atgatggttt tatgaataca gaagacgcct tattgatgga
    17041 tcttaatctt atttagaaat attgaggaga ctatgaactg gtttcaaaga aaaaaagacg
    17101 gtgttaatga aagcacagaa aagcgtgagt tgccgaatgt gtggagaaaa tgcgataagt
    17161 gcggcgaagt tcaatataaa aaagacgtag aaaaaaatta ttttatgtgc gtaaaatgtg
    17221 gttatcattt tcgcgtattt gcacgcacgt atattgaaat tatgttagac gaaaactcct
    17281 ttgtcgaaac agaatctaat atgatttcag ttgatccatt tcagttcgtc gataccaaaa
    17341 agtacagcga tcgcatccag gcgatgatta aaaaaacgaa gataaaagac gccataagaa
    17401 cgggttatgg taccatcgaa ggtcgtcctg taatggtggc aatcatggat tttggtttta
    17461 tcggcgggag catgggatct gttgtaggtg agaagatatc gagagctatt gatcaagcat
    17521 acaaaaacaa gctcccgatg attatcgttt cagcttccgg cggagcacgt atgatggaag
    17581 gagctctttc gcttatgcaa atggcgaaaa catcagcgaa gcttgcctta ctccatgaaa
    17641 aaggtattcc atatatttca ttgcttacag atcctacgac cggcggagtc acggctagtt
    17701 ttgcaatgct gggtgatgta atcatagctg aacctggggc tttgattggt tttgccggtc
    17761 ctcgtgtggt caaagaagca acaggaaaag atcttccgga tggatttcaa acagccgagt
    17821 ttttagttga gcacggattt gtagatttga tagtaccaag aaatgaactt aaaaaaacgc
    17881 tctcgaggtt gtttaaattt tttgagccga atacggacac ccaattcaaa ccaaccaaaa
    17941 gctgatacaa ataataattt tgaataaaat gaaacacata cttctgacta atgatgacgg
    18001 aatagatgct aatggtattt ttgcgttata taaagaatta aaaaaattag gggatgtaac
    18061 tgtcgtcgca cctcagatag aacgtagcgc tgtcggccat gccatcacga tacacgatcc
    18121 tattcgtgtg cttgactatc atcggccaga aaaattcaaa ggatacgcaa tttccggaac
    18181 tcctgccgac tgtgtgaaaa tcggtgtaaa aaacttggtt cataaaaaac cagatttgat
    18241 agtttcaggt attaatcacg gatcgaatac cgcaactaat gtgctatatt ccggtactgt
    18301 ctccgccgct acggaaggta ttatcatggg cataccttct attgcttcat ctatgattgc
    18361 tttcgatcat aaaacggatc ttacatttgc cgcagaaatt acggcaaaaa tagcgcgtat
    18421 tgttttggat aaagggttgc cttcggatac actactaaat ataaatattc ctcccggtcc
    18481 tcgtacgtcc ataaaaggaa tcgcagtgac acggcagggt cgcgggcgat atgaagaaga
    18541 gtttgagcaa agaacagatt taagcggtcg tgcgtactat tggttgaccg gaaaaaaaat
    18601 gatattggat catgaagatg atgttgacga tgtagttgtc ctaaaaaaca aagtttccgt
    18661 gaccccaatc caaatagacc ttacggcaca taagtttttg gatgaattaa aaacgtggaa
    18721 tattgaaatt taaaatcgct ttgagaaaca aacttttaaa agtttacatc gtataaacgg
    18781 ttacttttcg gtaaccgttt ttaattacac cataaaagga gatccgcatg aaaaaaacac
    18841 ttcgttatct tgcgtttagt ctaatgctat cgggttttgt attttctcaa gataataaaa
    18901 aaccgagtcc tccggccaaa tcagagcaaa aagtaggttc agaaaaaata tcaattaatt
    18961 atagccaacc ttctgtgaaa agcaggaaaa tatggggaga gcttgttccg tttggtttga
    19021 caaaaaatcg tgacggcaag atgatacctt ggcgtgccgg agcgaatgag aatacgactt
    19081 tcactgtaag caaagacgtt aaaatcgaag gccaaacttt gaaagccgga acgtatggtt
    19141 tgcatatgat accaggagaa tcggagttcg ttgttatttt taacaaaagc tcaacagctt
    19201 ggggaagtta ttcctatgat caaaatgaag atgctttacg cgtaacggtt aaaaaagaaa
    19261 ctgcaaaatt tcaagaagtt ctaacgtatg gttttgagag tgtaaccgat aaatcatgta
    19321 cggtttattt gcattgggaa ttggtaaaga taccatttaa aatagaattt tgatttatac
    19381 atttaagatc gactctttcg aatatgtttt tgtttagtaa aaagccccgc taataaattt
    19441 aacggggctt ttcgttattg ctgttttttt atcatttttt tcttggcctt cctggtcctg
    19501 gctttatttt ctttgtcgtt tgacttttct tctgcgtttg tgattttagt ttcaggcgaa
    19561 cgagtttatt cttttttggt cttccaggtc ccggtttgat agtaatctta gtttgcgctt
    19621 ttttatttcg gttaatgtgt tcattggact ttgcacgggt gttttttatt tgctgctttg
    19681 ctggttttgt cgttcgtaca cgagcaactt tggttttata tccttttgga cgacctcgat
    19741 tcgatctaat tttctcttca gctttaactg aatctgtttg aggtttagtc aaattcatct
    19801 tttcgatcat acgtgcgtac ttcataattt ctttgaattg accttcgctg tgaagccctg
    19861 cttcctcaag cactttttca gccgatccgg tagaaaggtg gtgtccatgc cggaatggat
    19921 gcagtgtgcg gttaagtcct tcattccacc ttatccactg atacattgtc ggcaatgtaa
    19981 aatctgtcaa acccattgcg tgagtacgga gatgttccgg aaaaatagcc tcctgttcct
    20041 tttcaggaag gagtttgaaa agttcagtgc tggcaacata aaaaatattc atgttcaaac
    20101 cggctttatc gattttttcc aaaacgccat tgataaacat actggctact ccgcagcctt
    20161 gcagtaccaa ggtgccgtga tattcacgcg cattcatatt ggctcggcga aacgcataaa
    20221 cgcctttagc tgcagcgatc gctggatcca atccgagttt gacacgatct gggactgcct
    20281 cggcgggtcg ggttacaaaa ggtgcaagca cggcgggtcg cttcatgatc ccttcgatca
    20341 taaggggcca cacttcgtcc ggtgaccagg gtgttagcgt tataagtttg cctgagggga
    20401 aatcttcgca tagaagttgc aaacactgtg ggtcggcatg tgtcggtccg tcttctccgg
    20461 ttttaggacc agcgtgccca ttgaccataa tccatgtatg catcggttcg ccggtacgtt
    20521 cccaatagtt ttgctgaccg atcgaatgac accgcgcagc ggtatgttcc atcgcgacaa
    20581 taaaagccgc gtacgaggcg ctgatgccga tatgattacg ataacttgaa acaccggaca
    20641 taaaagcacc catcgcgtct tcacaaatac cacccacagg tactaaacgc gcacccggat
    20701 tggtttttgc gttaaacata ccgtccggaa atccttttcc aacagccgca atgcttgttg
    20761 attcgaaaag atcggcggcc gttgctacta aggcgccatt tgattttttg ttaagataat
    20821 taagaacttt acctacttgt ccgcgaatgg tggtggatgt tcccggctcc aaacgaatat
    20881 cgtcaggcat tgcgcttaag ttgactgtgc cgtcataaat attggctagt tttggcgcat
    20941 gtgcgcggtg ggtacggtta tgagagtata gacgatcttt agataaggca aggcgtttgg
    21001 cagcttcttg cggtaaaaaa gaattttttt caagtacttc acgaatgact aaaagcgtat
    21061 tccaataaaa ggtttcaaca tccacagggt tttttgcatc gccggaaaaa cgaggaaaca
    21121 tcacaccaaa ttgttcttca aagggtttga tgaaattata aaactcaggc gaacaaaatt
    21181 tataaccggc accatgtgaa gctttaccag tgacaccata ggtataacct ttgaccgtgc
    21241 gatacacgat agctgtaggc atatcggatt ggagagatag agcaattttt tgagccgtga
    21301 gtacctgatg atagttgtgg ccttcgggta caaaaataac attccagtca ttaagataca
    21361 tgagctcaac aggatcccac tgtacgtagt cgccgtgttt atcaccttca gcggtgcatc
    21421 ggtctgaatc aatcgttgat tgattccaat caatgtgtaa aaccgcatta cgaagctgag
    21481 cggaagcggc tgcggctata gcttcataaa cacgacccgg tgtcatgccg ccttctcctt
    21541 cgagtaaatg tgtacgggga ggattttcgg ggtacatgtc cagtgcacca aaagcaagac
    21601 cgaatgccgc aggtacgcca acaccactag gtccagttgc cgttttcaca aaaggaacaa
    21661 tgcatgacgg atggccatcc agtgaaaccg atttaaattt ttcaaataat ggcgtacctt
    21721 gcgtcgggtt tttacgaaaa cctaaaagat cttcaaggcg caattgacgg cgttcggggg
    21781 caagcattcc gggattggct atgcgaatta actcattgcg taacgcccac atggcataca
    21841 agcccatggc tttatggccg gcagtgtagc aaatcatgtc gttgatatcg atatccggct
    21901 tggaaaaatc gtagtccatg gattggtata aaagaccctc gacgatacgt ccactggaca
    21961 gacttccacc aggatggccg gataagggta caaaattata gagaatccca cacattgtgc
    22021 ggtacacaag atcaagttct tcaaaaaact gtatatcttc tccggagatc gtcgaaggtt
    22081 tttgagccaa gatatcactc acatcataaa attttcctcg acggcgctga gaatgaatcg
    22141 gattcttaga agacggaagg tattccatgc gaaaactcca aattatgaaa tcatggttat
    22201 tgtatgtgaa ttacaaccgg caggcttgcg tcttgctcct aaatgcgata agttgatgct
    22261 atgccaaagc cggaaaaaca tgaagaaaaa tcagattcat atcgcatcgt aaaatacgaa
    22321 tcggatgaag cgagcgaata taatgatttt gaaattgcgc cgcaaacgcc atttacaacc
    22381 aacatcaaag ggcgttcttc cgggtttaat ccacccaatc gttttgatac gcagcatcag
    22441 gataaaagca tagacgatat tacagaagaa gatatagatc ctgaacgaac tgtacccacg
    22501 gaacttatcg aagacaaatc acgaactatt ctttcaaaaa atgacagtcc cgacatacct
    22561 tttacttatg gaatcaatcc gtatcggggt tgtgaacacg gatgtgttta ttgctatgca
    22621 aggccttcac atgaattttt gggtcattcc ttaggtttgg attttgaaac taaaattcac
    22681 gtcaaatacg atgccgcaaa actacttgag aaagaatttt tgaagccgtc gtggaaagcg
    22741 gaaccaattg ctttttctag taacacagac tgttatcaac cgatcgagcg aaaattaaaa
    22801 attacgcgtg catgtttgga ggtgtttctc aaatatagaa accccgtgcg aatgattacc
    22861 aaaaattatc ttataacgcg tgatttggac atcatttcac aactttcaaa gctgaattta
    22921 ttcggggcca tgatctcgat cacgacgttg gatacaggtc ttgcgcgtag attggagcct
    22981 cgcacatcgc gacccgataa ccgtcttaag gcgatcgaat tattagctaa aaacaatatt
    23041 cctgtgggtg ttttgacggc tcctattatc cccggactca acgatcacga aatacccgaa
    23101 atattaagga gagctgctga ttcgggagcg accagtgccg gatataccgt acttcgatta
    23161 ccgtaccagt tgcgtgagct tttttctgat tggcttacgc gaaatgagcc caataaaaag
    23221 gaaaagatat tacacgccat ccaatcggtg cgcggaggaa aactaaataa tacggaattt
    23281 ggaaaacgca tgcgtggcga aggtaatatg gccgaccaca tatcaaactt gtttaacctg
    23341 ttttataaaa aatacggatt cgataaaaaa gtggtacgcg gagagccggc gagttttcta
    23401 cgcggcgggg tggtacaaga aagcttattc tgaaaatata aatacaaagg aggtttatga
    23461 attggaacga cgccttgatg ccgatcatca aaaagtataa aaacaaaaag catcctttag
    23521 aatacaaaaa cagataccag cttttagtca tgacgctttt atcggcgcag gattccgata
    23581 agcatattaa cgaggtcggg tcgaggtttt ttggtgttta tccgtctatg cgcgaactgg
    23641 ccaaggcgac agaagatgaa atcgcgtcac acatacgaga tgtgcgcaat tttggtaaca
    23701 aagctaaatg gctagccggc attgcaaaaa ctgtcggttc cgatgaaaat ataccgacta
    23761 cgatggaaac cttgacatca ctcaaaggaa taggtcgcaa atcagccagt gtgattattc
    23821 gcgaatccgg aggaccagcg gaaggaatta tcgtggattt gcatgtcgta cgtgtggcgc
    23881 ccagagtagg tgttacaaaa gaagaaaaac cggaaaaaat cgaaaaagat ctaatggcgg
    23941 tcgttgaaaa gaaatattgg ggtgatgtag gtatggctat ttctttttta ggacgtgaaa
    24001 tttgtagacc cacgaacccc aaatgcgaag aatgccctat caatgaaaac tgttcatact
    24061 ttcaaaatca aatccgttct gcaaagtcta agaaaaatta gtttgacgtt gatcctttca
    24121 ggtaagtcat tcaccttgaa ttttcctata gtgttttgta acatggacaa aaattaaaga
    24181 gatcatacat gaagttagat gctttgtttt tcggtgcgca ccctgatgat gtagagttat
    24241 cggccggcgg gaccgtcgct aaattggtca aactaggata caaagtcggg atttgtgaca
    24301 tcacacgagg cgaggccggg acgcgcggat cagccgaaat gcgtgataaa gaagctgaag
    24361 atgcagcaaa aatactaggc gtacacgtgc gtgaaaacat agaattaccc gataccgagt
    24421 ttgtcaatga tcgcgccaat caactcaaag ccattcatat cattagaaaa taccaaccag
    24481 aatttgtttt ttgccaatat tggaatgatc gccatcccga tcacatgcat gccagtgatt
    24541 tagtacgcga atcgtctttt tacagcggtc tttccaaaat tgagacgtcg gacaaaggga
    24601 aaccgcagga gaagtttagg cctgcgaagg ttatatatta tgctgcgcgg tacgaattta
    24661 gccggataga cgggcatata tttatcgtag acattacaga aacgatcgat atcaaaatga
    24721 aagcaatggc tgcatttgcg tcgcaatttt tcaacccgac ttacaaatcc gacgaaccgc
    24781 aaacctatat cagttctccg gaattcactg atgcgattcg taaccgggcg gcgcattttg
    24841 gttcgttgat tgatacaaag tacggagaat cttttcttgt gcgtgaattt ccctccgtac
    24901 ccgatcccgt taaatttttc agagacggcg ggcaggatgt caataaactt ggtcttgcgt
    24961 aaacagataa acaatcaatt ttttaaatac cttattaaaa caaatgaata caaaaatctc
    25021 tatcgatgat ctttttgagc gaattcaatt agtaaaaaaa atcaacgccg aacaaccgct
    25081 atttccatct tacgagaagt attttgatcg taaaataaat acgatgagta gtgtatcgaa
    25141 attaaaccgt aaactattgg catgggatac gattttaaga tatcacggaa ttgatttaga
    25201 aaacaaaaaa gtcttagtgg tcggttgcgg ggctgggtgg gagtgtgttt atatgtatgc
    25261 tatgggagca aaagaagtag ttggcttaga agtaatggaa atgtttgtta aggggatgga
    25321 aaaaatgttc cccattctcg gactgcatcc aactgttaaa ccaatattac aagatattca
    25381 cacctactct ctagactcat attatgattt gatactcacc gttgatgcta tatcacatat
    25441 gtacgatcat aagttgtttt tgagttcttg ttttaaatta cttgctccaa acggctcatt
    25501 gctaatcatt gatgataata acgcacttaa ttatagacgt cgaaaagaag ttgcgaatat
    25561 ttgggaatca tgggaaaaat atggacttga tctaaaaagc aatgacggca aaacgatgca
    25621 tatcaattca tatgaacaag atcgctatga atatatagtc gaacggtttc ctgaattatc
    25681 taaagaggca aaatctttgg ctgaacgaac agctaatttt aaccgacagc aaattgataa
    25741 cgcaattttg aagtataaaa aaacaggaat catgcctaat tctgtgtatc gtcgaggtgt
    25801 tatggcttat gattcgaagt atgacatgcc taaggagaaa cagttcaatc cttatacact
    25861 ggctcatgaa ataaggagtg cgcgatttga aaatgcaata catttaccag acctcattac
    25921 aacagatact ttttttagaa aaaacgtagt caaaatgctc attgtattta agccgttcag
    25981 ttactttttc gtatcgaaag gattcagtgt ttccggtcgt aaatcatcac attaaatgtg
    26041 ttataataaa tacatgcaaa tctgtatata cgaagatgac cactacgtaa atttatatcc
    26101 gctcacactg acacggccgg tattcgaatt acgttgcggc gcttcaactc ttgccgagcg
    26161 catagctaga tatttcccag aggcagaaat tttttatcaa tgccgaccgc tgctggcttc
    26221 ggtgtatcat gagcaatatc ctgatcgccg gatcaatcat aaaatttccg gggaatgttt
    26281 gtttatcaac gggcgcgtgc ttatgaatga tgcattgatg caacaaatgt ctgagcatag
    26341 cggaggagag tttataaatc agggagttat agttggcttt tggaatagtg tgaaacctgt
    26401 aaaattggat gcagaaaccc ggatgataaa atatccatgg gatttggtga aatataatgg
    26461 agagtgtcta cgtgaagatt ttgatcgaaa caaatcgggt catacacagg caacgattta
    26521 tgcgggtgtg catttagtaa atgctgatgc gatcacgatc ggcgaagggt catctataaa
    26581 accaggcgtt gtgctagatg ctgagcacgg ccctattatg atcggcaaac atgttcaagt
    26641 gatgcctaat acagtcattg aaggtccgtg ctatatcgga gaccatagtg tgataaaaat
    26701 cggtgcaaaa atttacggca gtacatccat tggccctgtt tgtaaagtcg ggggcgaagt
    26761 tgaaggctcg atcatacaag gttacagcaa taaacaacat gatggttttc tgggacattc
    26821 gtatatcgga gaatggtgca atcttggcgc cgatacgaat acgagtgact taaaaaacaa
    26881 ttatggtccg gtatccgtta cgatcaatgg agcagaaatt aataccggtt ctttatttgt
    26941 aggcttgacg atgggagatc attctaaaag cgggatcaat acgatgttca acaccggtac
    27001 ggtagtaggt gtgtgcagca atgttttcgg tggagggttc ccccccaaag ttataccatc
    27061 ttatgcgtgg gtagattcgc cgaaaataac attgtataaa atggataaag cgatgcaggt
    27121 cgcaaatact gtaatggcaa ggcgcaaagt gacgttgggt gaaaaatata acctgctttt
    27181 gcagtggata ttcgagtttg ccaaagacga acacgctaaa ttatgaatga atggttaaaa
    27241 aataagaaat ttatagtgaa agcgcttgga gcgcttatat tggttttgct tacggttcac
    27301 ctcattattt ccatcacacg tgtttctaag caagaagatc cttttgctta tatcaaagaa
    27361 gaacctgtag ctgaacaggt tattcctgaa ccacgacgac ttacgatcgg tgtcagtttg
    27421 gaacaactta ataggtatgt accttttgat gaggccattg attcgctcct gactcatgaa
    27481 tttgatatcc aacctggttt tatcaaaagt gaacgcattc aactggaaaa tgtcaactat
    27541 atccgtaaag tcatacagct tccaaagaat tttccaaata cgctttttgt gctcgatatg
    27601 aatgttctat ccgagcagcg ggattgggat atctttagtg tcagtgaaaa actcaaccgt
    27661 caggccggcg ccagtgactt tactatggat atcggtcgtc gtaatctgat atacagtcgc
    27721 cttgaattta ctgtaaatcc cagattgcgg cctatgggca aagaagtgtt ttttgtggtg
    27781 acaggttttg gaaagaattt tgacgatctg acgcgttcat ttgttgaatt gcctgaaagt
    27841 tttgccattc ttgtacctaa aacggaatcc aactataaaa tcatagccca tgaagcagaa
    27901 cgaacgggta aatctgtgat gacctatatg ccgtggtcaa aatatattta ttattttgat
    27961 gaaaaaggcg gtgatcaaga aagacttacc caagcgttat atgaaaacct tgaaaaagcg
    28021 gcgaaccgag cttttatcgt ctgtgaatat tcatctgcaa cctaccaagt cctcaaaaaa
    28081 gaactccccc gcttagctaa aaaaggctat atcccccgta cgtacattca aaagtggtga
    28141 aatcgactgc aagttgtttt gttgtgtgaa tgattttaag taatacgtgt aactatctaa
    28201 aacataatcc ttttagaaat aatagaagaa acaatatatt tccactagtc tccatgaagc
    28261 cccgctttat atcgcggggc taatttattc ggtattaaga aatatctaat gtgtctttta
    28321 cttttctaaa atccacctaa gcgcattgag taattccttc tgtttcggtt caggtatcgt
    28381 gtgacctatg tttgggtaag gaacaaaacg aacactatgg ccggattggg ctaatgtatc
    28441 cgctacagaa ctaatatgtg gataattttg ttctaacgtt ccatgtccaa tataaaaccg
    28501 agctttttca cctgagttaa ataacgagga atgcgtatcc aaagaggctc caaataaaac
    28561 cgtccctcga ttgggtattg tgttctttaa ggtcagataa gccgccagcg atcctccgga
    28621 tgaaaaaccg gcaaaataaa tttgtgtgga atctatctga atacgttttc gtacatcaac
    28681 taaaacattt ttgatataac gctcggtagc ctcattgcga ttccacccaa aacccatatt
    28741 cattttttct aatccttgcg gcgttactag gatgagacca agagaatccg ctgatttctt
    28801 ccaaaaatca tgaaaggctt ctgccctatc tcctgagcca tgcaaagcaa taagcactgg
    28861 ggtcgggcgt tttgattcgt atttattagg aactgtaagc agtacaaaag aatcaggaaa
    28921 atcagttgca tgaaatttgt gtgtcgcacc cggtggtatt tccggaaatc cggtggagca
    28981 agattccaaa aaaaataaaa ttaagaatag ataaaattta tttcgattca agttgcaccg
    29041 ctggaataat atagtcagat aaaatttttt cgctttgtgc gtgtgctttt ccagtaccgt
    29101 accacgtact tgtgattaca accgtgagat gtagctcggg aaacacggcg attttacttc
    29161 caccgttacc ggccatataa tacccaaagt aggttttgtc attagagcca aatttttgta
    29221 accagcataa ataaccataa tctgtatttt cacgagcctg agcatggggt tttgtggatt
    29281 tttctatcca cgatcccgaa atgatacgct tcccattcca tatacctttg ttttcataaa
    29341 gttgagcgat tttgagtaaa tcgcggcttc gtaaaccaag cccaccgccc gtcataggca
    29401 ttcccgtagg cgtgatttgc cacgcggttt gctgaatccc caacggatcg aacaaatatt
    29461 tttttgcgaa tgcgtcaaca cgcatattgg agctacgttc caatacacct ccgagcaaaa
    29521 ttacaccggc tgtacaataa ctaaagcttc gtccgaaagg cgagtcctga ggtttgggat
    29581 tccatgccgc aaacccccga atcggcaaat ccagagcgaa ctggatgtag tcctcaataa
    29641 gatacatgcg ctcttcgttt ccgttcgaga actgattatc gtcatcgcat tcgagcaaag
    29701 agctcatggt aagcaaatct tctatggtaa tttgattttt acgcggatcg ggattcagga
    29761 tttccttttt gtctctgaaa tacggaaata caggcgtttt ttctgaagga ataaattgct
    29821 gatctatcgc taaaccggta agtattgctg tgatagtttt ggtagcgctg cgtgtattgt
    29881 gaagcgtagc ttcgtcggcg ccgttataat aattttcaaa aacaagcgtt ccatggcgtg
    29941 cgatcaaaat actcgtaatg ttttttaaaa caccctgatt tgcagcgcta tccaatttga
    30001 gcaaagtggt cggtagtata tgcatcgaag aagcgtcgga aactttgata ccgtcgtgcc
    30061 gatctatagg aacctgagaa accgaaggaa tactcgtaca aaggaccatg catagaatta
    30121 cgaaaatttt cataatgccc ctctatgttt tattttagtg ttaccataat ataacacgtg
    30181 cggaggctta gaaatagaac gtatttaaca tggttcattg atcttgcaca actctcggcg
    30241 atagcgaagc ggcgttattc cggtaacagc tttgaaacaa cgaacgaagt ggctttggtc
    30301 agaaaaaccg cattgcaacg caatattagt aagtgacaaa gaaggatcat acatccgttc
    30361 gatggatttt tgcagacgta tttggcgaat atatgtaccc agcggaacac cgaaatattg
    30421 agtgaaggcg cgcgataaat gtaccgggtg tatatgcaga gtcatggcca ggccaagcaa
    30481 tgtatgcgtg tcaaatgggc gggcatgcaa aacatctcgt atttgcgtta cccacaaagg
    30541 cttatttgag tgaaaagctg tagttttaga tacatgcaca agagcacggc aaagcgaatc
    30601 ttctatagaa actacagaag ccgtatccgg gcaaccgagt tcgcgtgcca tagaatagaa
    30661 tgcgatacga actgcaggat cggtaatctg aaaacttcct tcaaacatgc gacgatccag
    30721 ttccagattg tcaaaccaag actgctcgat ttcgatatgg aacccttgag tgaattcagg
    30781 aggtttaatg ttacagtgtg actcttgcca ataatgaaac aataaatcac cgggagcaca
    30841 cctcacggtt ttttttcgat cggtttcgat cagaccgcca cgaagaagga atgtgaagta
    30901 agcattttgg tggtaatgcc aatctacata atcatgggta taaaccgttt tggtaagcgt
    30961 aataccgtcc agatgcataa ttttgtcagt ttcaccaaaa aactgacctg tttgcatctc
    31021 tttcatcgtt cagtttcctg taatcgtgtc aagaatatgt aaaatgtgaa acccattaga
    31081 atgtttcctg tgtataaaaa ttctgatatt taagattctg tgtgcgccgg tgataatgta
    31141 tgaagcaaaa aaatacaaat tttgtgcgga gttaaaaacc actacttctt agaaagccga
    31201 tacaagtcat ttcatgtctt gacaatcata aggttattgt cttctgttac gcgtttaata
    31261 atgcgaagtt ccatgacgaa gtagttaaat catttttatg agattttgat ttcttcgata
    31321 ccattataaa acatcaaaat cgctgttaga tatttgcaca attgaggcgg gtaagcttcg
    31381 aaacaatgtt agccgatgag gcgacgatga tagtttactt gtcctagatg gtagtttaaa
    31441 tgagcaagca ggtgcaagag catgtgttcg acagttactg ttttgccatg cttctcaatc
    31501 ggataatttt gatacagcac ggagtcatcc atacgggaaa acgttgtctt cgccattgca
    31561 atcgttgtat ctatgtcctg tattagtttt atgcgtggaa catcggaatc agaaaattca
    31621 cgttcccttt cgcgaatgta tcctgttttt ccgagtacag caccaatgaa atgattgata
    31681 ttaccgacta gatgcagcgc aagattcccg gccgtatttc ggatatcgcc ttcgatcttc
    31741 cagatcgctg tttcgttgct gtaacttgcg atttcttctt tgagtttcag caaatcgcgt
    31801 tcaaaaaatt cagataaagt ttcaatgatc atgatattac cgggataaat aaaatatagt
    31861 tagttgacac taaagcgatc caagtcaata cctgttcctg cggtacgtgt tcgcgatgct
    31921 ttacgggcgc gtttttcagc ccattggcgt aatgccgaaa tctgttcggc catgacacgc
    31981 gagagcggca ccgtggcccg caggttattg gaaatatcgt cggttgtcaa ttcacgtcca
    32041 gcctcaaagg catcgtacaa accggcaatg atagcttgtt cgatctcggc accgctatat
    32101 ccttcggaca gcaaggctaa ctttacgacg tcaaacgaat ccgtagtacg gtggcgcttc
    32161 tgaagatgaa tagtaaaaat agcttcccgt tctacttgtt ccggtaaatc cacaaaaaag
    32221 atttcatcaa aacggccttt acgaagtatt tccggaggaa gaagagttat atcgttagac
    32281 gtagcaatta caaaaacagg tttctttttt tcttgaagcc aggtggtaaa agaaccgaat
    32341 actctcgcgg ctgtgccacc atcagaaaaa ttggaacttg ccatacctga aaatcctttt
    32401 tcgagttcgt caatccataa aatacaagga ctgatggatt cggctacttt gatggcatga
    32461 cgcatattgg cttctgacga tccgacaata ccgctaaaaa cactgcccac atccagtttg
    32521 agcaacggca gattccaatg tgctgctacg gctttagccg tgaggctttt tccgcaacct
    32581 tgggtaccaa ccaacaaaag ccccttcggt gtaggcaatc cgaattgggc ggcacgttca
    32641 gtaaaagcct ccgcgcgttt ggtaagccat tgtttgaggt tttcaagtcc gccgacttgt
    32701 tcaaaatttt catccggaga aaaatattct agggtgcttg atttcctaat gatctgcttt
    32761 ttctcttcca aaacttcctg gatcgtatga gcgttgatcg ctttgctgtg tactaccgat
    32821 ttagccagta cattttcaaa ttctaccaag gttaaacctt gtgcggcttt caagagtttt
    32881 tcagaaatct gcggtgtcaa ttctaggact acgtcgcggc gcttatggag aaccttaagt
    32941 gcgttttgaa aagcgatatt gagttccgtt aaacccggca gatcaaaatc cataaccgcg
    33001 atatctttgg atagctcgag gggaatcgtc atttgcggtg caacgataat aatcgtttta
    33061 ttgcttttgc gaagtaactt ggaaagatcg cgcaattggc gccgaatacg gtggttttcg
    33121 ataaacgcgt gaaaatcaaa caaaacaaaa atgccgttat cttgagaagc tgcgatatgg
    33181 ttaagcaaca cctcaggatc ggatttggat tcgtccaaat ggtctggcaa cacataattg
    33241 tacaagccct gtgtttgcgt ccaaacaaaa agcattttgc gaagatgaat agcgatctgg
    33301 tcgagcgttt tgaggatgcg ctcctcctcc catgagacca aataaatcaa agggtagcgc
    33361 gcttgtatgt aaacttcgag atcgttttgc ataagtaggg tatgcttttt ttagaaagct
    33421 aaaggttcat caaagaaaat acaagaataa acattactat atgtgtggtt aaaatcgtta
    33481 caaagcgtca aaataaaatg gcaataaatc cgtcaaagta gatttttgaa aattattttt
    33541 taaagttaaa ctaaataata acagtttttt atagattggc acaaagcctg cataaacgtg
    33601 taggtaatta ttagcgacca aattaaaaca gtatttaaat aaataaggag ttttctaaag
    33661 tatgaaacga ttatggacat tgctgctatt ggtttttatg attgcgtgtg aaaaagaaaa
    33721 aagtgatggc acactaaccg atgaccaaaa agaggttgtt gaagcggcgg tgaaaacaac
    33781 agcgttggtc agtcaagtgg atgacgagtt ttcggacaat gaggaaataa cttccgaaaa
    33841 tggatataac ggcgccggtg atgaatccga ctatacagcg agttcgataa taaaagatat
    33901 atctgacgaa catcttatga aaagttcttc gtcatttacg ttcgtgggtc gtgataccat
    33961 cacacgtaat tatccggatg gcacgcaagc tacgatcata cgtgtgtttg atccaacgac
    34021 actaagatta acaagtattg acgtagcaac caaaaatttc ccaaacagca gtcgtgttga
    34081 gaagaaattt tatcgtgcga catttacata cgaccctaat gtgatcatta ctcagaatac
    34141 tgtgatgaca tcaagtttta aggattctac gattatcaaa acgtatgagg aataccccgg
    34201 gtttacacag attacgaacg ggacaataat tcgtaatgtc ggacgcgtga agtttgccga
    34261 cagaaaacta agttattctg ctacaacaac cggttttgcg acggtgtcag gcatatccaa
    34321 tcgagttaca agcatttcta ttgatccggt tggaggcact tcccataagg aaacaaccac
    34381 taattatacc gatgcatcat caagtacatc cgagataaca tatacccgta ctgtcaatgc
    34441 tacaacgggc gagcgaacgc tgagcggaac tatggatata acacttcgtg acggtacaca
    34501 ccggtattcg gtttcaacga tgaatatcac ggccgatagc agcaatcttt ctaagagcgg
    34561 aaccatccaa attcagacag atttcccggc cggcgatagc ctttatcgta aaatcacaaa
    34621 agtaatcatg agtggagcgg tatcaaccga ttcgtcaaca ttctacttcc ttgatggtac
    34681 gacatattct acggtattga ctatcacgcg aacttcagcg cctaccggca atggtacgcc
    34741 ggagggttta tcgtatacta ttacgggttc ggatagccgg gggagaacac tcaatggaac
    34801 ggtaacagga aaacgttata attttgaaac cacgggtacg atcacagaaa gtgacggttc
    34861 gacctcccag tttactctga ttcgtgacgg ttccagtgta acactgacat ttaccatcac
    34921 caaaaccgat gccgctttat catccacagg ctcatttgtc tttgatttac aaaataaaac
    34981 cggcggtggt acgatcacca atggtgacat tactatggtc tatgttttta caggtatgtg
    35041 tgaaggatat tattatccgg aaaatcacga aaacttaaaa caagacatcg aaggctgtat
    35101 gtttagtctg ttgaaattat aaaaccctct ccggtatcat gttaattcaa aagccgacgt
    35161 attttgcgtc ggctttttta ttcaaaactt attcgtcatc cccaagcggg tcaaaatgaa
    35221 tcaatccgta ggtttctgcg attttttgga cgagagctat attctctgca tgaccagtac
    35281 catcggcggt tatcaaacct ttgataggca taccgagaag tgaaaaatca cctaaaatat
    35341 cgagcacttt atgacgcgtg gcctcgttgg gatagcgcgg aggaattttt gataatatct
    35401 tataaaaggg cgtttcatga accgcactac gtgcatcttc accgataatt tcttccatca
    35461 agtcccaatt gggttcggaa tccaaaatta taaaaccggc gttaggatcc aaaccgcgaa
    35521 taaatccctt acgatatagt aattgtaatt cacccgcgta agcaaatgta cgggctgacg
    35581 cgatttcttt gatgtaattt ttaggtgaac taaaaaaaga aaaagtatca atggcacgtt
    35641 tttgaaaacg ctgagtataa gtgacatgaa aattttggct tggttcgatt cgaataaacg
    35701 ctccattgtc agggttgcga tgagtgatgg tacgatcaat ggttagtgct tcattttggc
    35761 tttcagtagt aagagtgtca gcgagcgctt cgataaaagg cattgcgctt ccatccaaga
    35821 tgggaatttc accgccggtt acggtgattt cagcaccgct gagatttaat ccgtagatgg
    35881 ctgagcagag gtgctctatg gtacgtaacg aacgatcgcc gacagaaatc gtagtagcgc
    35941 gttgggtgtc ttttacatgt tggataagcg caggaattgg cgttccctca acattaaaaa
    36001 gtacaccgtg ttcatgcggg cgaataagaa tacggcttgg tttcccactg tgaatgccta
    36061 cgccgttgat agttatttgt tggttcatag actccatata tttctttgta cataacttaa
    36121 taaaaacaat ttgcggaaac accaaaataa cattattatg ccgcgctgat ggatgtaaat
    36181 aatttgtagt ttacagagca ttaatttaac ccgtttcata tatgaatatt gtacataaca
    36241 gcggttggat cgaagtgata gccggaagca tgttcagtgg gaaatctgaa gaacttattc
    36301 gccgcatgcg ccgggcagaa attgccaaaa tgcgtgttca ggtttttaaa ccgcaaattg
    36361 attcgcgtta tagtgaagat catattgtgt ctcacagcga acttaaaatg ccttcggaag
    36421 ttatcgaaaa agcgtccgaa atcatggaga aacttgatcc tagaacggac gtcgttggcg
    36481 ttgacgaagc ccagtttttt gatgcttcga ttgttgaaat atgtaataaa ttagcataca
    36541 tgggtaaacg tgtgattgtt gccggattgg acaaagatta tctaggccgg ccgtttgagc
    36601 cgatgccgca gcttcttgcg gtggctgaag tggtagacaa acccatggct atctgtatgg
    36661 tatgtggtgc accggccaac tatacacagc gacttgtcca aagcgccgat cgcgttttgg
    36721 taggtgcgac aggtacgtac gaagcgcgat gccgccgtca ttttgatcct cctaaagata
    36781 cgaaataatt taattggctt aagcgactca aaaaatgttc attgattttg tacaaataca
    36841 agtgcgggcc ggtcatggcg gcgacggctg tgtaagtttt cgcagggaaa aatttattcc
    36901 caaaggcggc ccggacggcg gtgacggcgg caaaggcggc gatgtagttt ttgaagccga
    36961 tgaaaatctt catacgcttc aagacatcgt tcatcatcgc atttttcgtg cccagcacgg
    37021 acaaaaaggc ggcagccaaa atatgtccgg tcgttttgcg gatgatattg taatcaaagt
    37081 accagtcggg acgatcatca aggatcaaga aaccggtgaa atgctgtgtg atcttaccga
    37141 aaacagacaa cgtgcagttc tcgccaaagg cggtcaaggc ggtaagggaa attcacgttt
    37201 taagtcgcct accaatcgca cgccgcgcga atttacgccc ggtgaacgcg gtgaagaaaa
    37261 atttttggat cttgaactta aagtattggc ggatgtagga cttgtcggtt ttcccaatgc
    37321 cgggaaatct actttgcttg cgtcagtaac agcggccaaa cctaaaattg cgaactatcc
    37381 ctttacaaca ttgacgccga atctaggtat tataaagatc ggcgaatatc gctcattagt
    37441 attggctgat attccaggct tgatcgaagg cgctcatgag ggtaaaggtt tgggtgttca
    37501 gtttttgaag cacattgaac gtactaaagc gcttgctttt ctgattgatg ttcaaaacgc
    37561 aaaccccgaa aaagattatg tgacgttaaa aaaggaactg aaggctttta ataaagaatt
    37621 gctcaatcgt cctcgccttg tcgtattgac caaaatagat acggtagata ctttgcccaa
    37681 acgaaccaaa atgaaagatg gcacacccgt attgatgata tcctcggttg cccaaacggg
    37741 gttagaaaaa ttggtcaaaa cactctggaa aatgctgtag ttttctaggt ttaagattta
    37801 ttgactattc ttacacatta aacttaaagt gcattacgtc accgtctttg acgacgtatt
    37861 cttttccttc ggtacggagc agacctttgt ctttacaggc ttgttcgcct ccgtgtttga
    37921 caaaatcttc ccaccaaatc acatccgcgc atataaagcc tttttcgaaa tccgaatgaa
    37981 ttactccggc ggcctgagga tttttagtgc cgcgtgtgat cgtccatgca cgcacttctt
    38041 cgacgccggc ggtaagaaaa gaaatcatat ccaataacga atatgtttca cggataagcg
    38101 catgaagccc cggttccttg agacctaaac tttcgagaaa tcccttacgt tcttcttcag
    38161 gtaattgctg aatttcggat tcgatctgcc ccgaaatcac aacaacacgt gcattttctt
    38221 tttcggcaat ggagcgaact tgtttgacat aattattttc cgtgagtaca tcgctctccg
    38281 atacattgca tatataaagc acgggtttgt cggtaagcaa aaaaagatcc gcgatagctt
    38341 tgcggtcatt ttcggataaa ctcatcaggc gaaccggttt tccgtcattg agttgtactt
    38401 tgactttact cagcacatca aaagcaagtt tcgcatcctt atcaccgctt tttgcagttc
    38461 gttcaacttg ggaaatgcgt ttgtttacgg attcgagatc cttgagtaca agctcggtat
    38521 caatgatggc tatatcacgt tcggggttaa cactgccttc gacgtggaca acatccgcgt
    38581 tgtcaaaaca acgtacgacg tggccgatag catccacttc acgaatgtgg ctaagaaact
    38641 gattgcccaa tccttccccg gcattggcgc ctttgacaag acctgcgata tcaacaaaat
    38701 tcacagtcgt ataaagtaca cgtttgggtt tgacgacttt gatcagagcg tcaatacgat
    38761 ggtctggcac tgtcactacg ccgacattgg gttcgatcgt tgcaaaagga tagtttgccg
    38821 ccagagcacc ggctgcagta agtgcattaa aaatggttga tttacccaca ttggggagac
    38881 cgacgatacc gcaacgaagt ggcatatagc aaatccttat atgatatttt taattttcga
    38941 ttaccttaaa aaaaccgcgg caaagtatgg gatgcaaagc agaaaatcta taaaaaaata
    39001 gtcttatatg aacttaatcg tcttttcgaa tatgcagcgg tttgccattt tgccaaaggc
    39061 cttttttgtt tgatttggca taacggtatg cttcctcaag acgcgatttg tatttgtagt
    39121 taggcgaata aaataacggt gtggcgtaac cctcacggat gatggtctca ttgatcatcg
    39181 tatcgtcttc cgtaaaaaga taagcgagca ttcggccgta acgatctcgc gtatcgcgat
    39241 cggtttcgat gcggaggatt gttccgagag gaaaattccg ttccacaaaa cggcgtgctt
    39301 cgattccctg ccgaaccatc gcatcaagat cagcagaatt acgagccgcg tcttttttgg
    39361 ctttaagatt atccttggat tcgggcgtgt caattccgat caaacgcact ttttccgttt
    39421 tcccgttcac ttcgatcaaa aatgtatcac catcgctgat tttgattatg acgccgtggt
    39481 tttccgagcg gccttcaacc gatgagatgt catagttttt aagataaaaa aacaaacaag
    39541 ccaaactcaa aaaaacaata atccatttac gcataacttc aactcgcctc gattttttga
    39601 gattgcaaga cgaaaaaaaa ttctctactt tgcgtcaagt taaactgtcg cgttaccaaa
    39661 cgaaagtaat ttcatgtcta agttcgtttc gctgaaaacc gcacttcgcg agcgtgtcca
    39721 cttggaaaat gctctgaaag aaatgggttg cgaaatttct aattccaaaa aaataaaaac
    39781 ccttttaggg cgttcgtacg atgtcgatct aacggcaaaa acagcatttg gaatgatcgg
    39841 ttttataaaa aaccgtagcg gtgaatttga gattgcgggg gatgatatgg ttttggcttc
    39901 ggataaagat tttgtcggta aactgacaca gaaatatgcc tacagtcgtg ttgtttcaga
    39961 agcgcaaaaa gccggatttc agttggtaaa agaatcagtc aacgaagatc aatcggtgcg
    40021 gttggtttta agaaaatgga acgggtaggg ggcgctatga catccgacta taaagaaatc
    40081 gtttttacaa taactcgcga cggctcgctg gaatatgcga tcaaaggcta taaaggcggc
    40141 gcgtgcgaag atctatccaa actttttgaa tctatgggcg ctgtgatcga agaaaaaaag
    40201 acatacgaat attacgaaaa agacgaagac gtcactcaaa aaattcgact ccagtcgtaa
    40261 aagcgttttc gttttgtttg atctctgtgg tttctcattt agttgaattc cgatacgctt
    40321 gatttataaa ataacgttcc ggctttgcaa ttgttcacct atggggagtt gtgatgatag
    40381 cggtgaaaca tgacaagcgt ttttttattc gaaacactct gattgctgtt ggctttgggc
    40441 ttttgtccat tggcatgagc tatatccgct ttgttattcc cacgttcaac tttatgacat
    40501 ccagcagcct caatgaggtt gccatactca tcagtatttt attccttccg cattggggat
    40561 ttgtcatcat caccggtgcg atgacggcag tgaatgcagc caacgaactc caattatttt
    40621 cggctgtttc acatggcttt tcggctgcaa tggcgtggct tgtttatcat cgtttttttt
    40681 cgcgattcac caaatcacgt caagaaccca tcttgtgggt gatggttgtg attctatact
    40741 attatctctt cctgttaccg cagattgtcg gattgtatct tctttttgat cccaatccga
    40801 tgccggccat gcacatcact tctgcttttt ttgttaatat gactccggag ttatttgcaa
    40861 cacttctgat cactttgctt tttagtatca tgcggcaaga gcttctgagt cgtcgtgaaa
    40921 cggagcgtgc gttagcagaa aaagaagaac gttatcgtat catagctgag cagacaggtc
    40981 agttgattta tgatcttgat gtaacgaccg gtgcgatcac ctggaccggc gcgatcgaaa
    41041 tcaacacagg atttaccaag gaggagtttt tacgggttga tctgaaaaca tgggaaacaa
    41101 tgattcatcc cgaagacagg cctaacgcca tttctctttt agatgaagcc atggcttctg
    41161 gtaatcggta tcacgtcgaa taccgctttc gaaaaaaaga cggtggttac gtgtttatgg
    41221 atgacaacgg tctttttctc cgtgatgaaa aaaacacggt ttaccgcatg ctgggcacga
    41281 tgactaatat agacgaacgt aaaaaggcag aaaaatctat taaggaaaac gtcgagcgtt
    41341 ttgaaatcat cgctcgagcg accaatgatg cggtttggga ttgggatgca cggcaggata
    41401 aagtatggtg gaatgaatcc gcccgtaagc tgtttgccgt agaagaatat tacccgcagg
    41461 acaattaccg gctttggcaa gagcgactgc atccggaaga ccgtgaacgt gtaacccaaa
    41521 aatacaaaaa cgttttagaa agtacgcaga atttctggag cgatgagttc cgctttcgtt
    41581 tgccagacgg aagttacggt tacttttatg atcgagctta catattgaga gatgagcaag
    41641 gtcggccaac tcgcgtattg ggctcgatga ccgatatgac ggagcggaaa aaactagaac
    41701 aaaatctgat tcaggctcaa aaaacggaga gtcttggccg tgttgccggc ggtatcgctc
    41761 atgacatcaa taatatgctg gctgtgattt tacctacagc cgaactgttg cttaaccaaa
    41821 attatgataa gcatactgtt catcagcacg ccgacgtgat cgtgtcatca gcgcgtcgtg
    41881 cgtcagatat tgtcaagcaa cttttggttt ttgctcgcca aactccgtcc agtgcagcat
    41941 tgctcaacat caataatctg gttgatgaaa cccgaaacat gctggatcgg tttcttggca
    42001 aaaatattac gatcacctta cacttagaac ccgagattcc ttatatagat gccgatgcaa
    42061 cacaggtaca gcaaatcatc atcaatctgt gtgtcaacgc ccgcgatgcg atgaataata
    42121 aagggagtat cggtatcgaa accaaaatgg ttgaactgga tgatacagaa tcggcaaaat
    42181 ataatgtgcc ggaaggtctt tatgtacaac tatccatagc cgattcgggc acgggcattc
    42241 ctccagaagt aatggataag attttcgaac cgtttttctc gaccaaagat ataggcaaag
    42301 gaaccggatt ggggctggct gtcgtcaaaa caatcattac caatcacgga ggatatattc
    42361 atgttcattc gcaaatcggt gtaggaacgc gcatggagct tggtttcccg gtttcccagc
    42421 gcgttacgat accccatgtg caggcgcaaa aaccgcaata tgaaaccggc accggcacca
    42481 tactgctagt ggatgatgaa aaacccatat tggaaatggg agaaatggtt ttgaagcgtc
    42541 tagggtatca atgttatacg gcggaaaacg ggtatcgcgc cgttgaagtc atgaaccaaa
    42601 aaccggttga tctcgtaata ctcgacgtgc aaatgcccgg catggatggc atcgaaacat
    42661 tggaaaaatt gctcgaaatt aaaccggaat taaaattcct tttcacatcc ggatatatcg
    42721 gagcggatcg tttacaattg ctccacagta aatatcccga ccatatagtc aataaaccat
    42781 atacggtcga agaattatcc aaagccataa agaaactact tgatacccaa tccgccggat
    42841 aaccgctgta taagtatagc agaaaccaca gaatttcatt taaaagctta atttgttttc
    42901 gtagtttcat caattgatta tttgtaacaa cctcataaat aaaaagctta aaatgaaaga
    42961 caaaattttt atagccaacg gtcagggatt ttggggcgac agcattgatg cgcctgtgca
    43021 tttagtccaa ggcggccaat tggattatct cactttggat tatcttgccg aagtcacaat
    43081 gtccatcatg caacgtcaaa aactcaaaaa accggagctg ggttacgcta ccgattttgt
    43141 gcaacttatc gaacgcatat tgccgacgat cgtagaaaaa aacattaaag tgatcgccaa
    43201 tgccggcggc gtaaatcctg aagcatgtcg tcaggccctt tttgccgtgg ccaaaaaatt
    43261 aggtatcaaa ggtctgaaga tcggcgtcgt ctatggtgat aatattcttc cccgtatcag
    43321 tgaaatggaa actaaacaaa ttccgatgaa gtccatggac accggtgaaa gtttgtatga
    43381 tctgatgaaa caaggccgcg acatcttaag cgccaatgtg tatatgagtt ctcttccttt
    43441 agtcgaagct ttacaaaaag gcgcgcagat cgttgtcgca ggacgaacta cagataccgg
    43501 attggcaatg gcgccaatga tatatgaatt cggctggtcg tttgatgatt ggaataaatt
    43561 ggccgcagga acagttgcag gtcatatcat cgaatgcggt gcgcaatgta caggtggtaa
    43621 ttttacgcgg tggcaggatg tgccagatat gtggaatatc ggttatcccg tcgtggaggc
    43681 ctatcctgac ggaacatttt atgtcagtaa acatgaaaac acgggcggga tggtgacggt
    43741 ggacaccgtt tcagaacagt tggtgtacga aatgggcgac ccgcataact atatcacgcc
    43801 ggacgtcatc gctgatttta catccattcg tctggagcaa gccggaaaag atcgtgttaa
    43861 agtattcggt gtacaaggta aaccttcaac ggagttttac aaagtcagcg ccagttatct
    43921 caaaggatat aaagcaacag gacaacttac gatttccggc cctgatgcgt tgcccaaagc
    43981 taagttggcc gcagaagttt tatggaaacg tcttacgcgg gcaggaatgg aatacgaaga
    44041 aacacacacc gaatttctcg gcgtaaacac gtgtcacgaa ggcatggtcg ccatacccaa
    44101 tcccatcaac gaagtggttt tgcgtgtcgg tgtaaaagat aaagatcaga aaaaggtaga
    44161 tcgcttcggc aaggaaattg cgcccctggt taccagcggg cctccgggag taaccggttt
    44221 tgcaggaggg cgtccaaagc cacaggaaat cgtggcattt tttccgtgcc tgatacctaa
    44281 acatctcatt gaaacaaccg ttagcgtgga agaagtgtaa ggataatcgg acgtctcagg
    44341 catgaaaaaa atactgtata tcgaagacga tcccgtcgcc atgacataca tgacgcagct
    44401 tattcagcgc atgggtcata cgattataac agcgacgacc ggcgagtccg gtttggccaa
    44461 agcgtttgcc gaacgtcccg atcttatttt gatagatctt ttgttgccgg gtttggacgg
    44521 atttgaactc acgatcaaga tgcgcagttc cgaaacgctc aagcatacac ctatcatcgc
    44581 tactaccgca acaccgaatg aagacgatca acaactcgca accatggccg gatgcgacgg
    44641 ttttttggtt aagccgattg aatacgaagc tctttctgat ctgatccgcg gttgctttga
    44701 ttctgagaca catacaaata agaatgttag tctcgaatcg gaaaatattg agttgcgacg
    44761 ctttacactg cgtctggttc atcgcctgac ggataaaatc aacgagatct cctacgccaa
    44821 caaagaactt caaaaaacca ataatactct cgatcgtctc atacaggaag ttcgcaccaa
    44881 taatgccgat ctgatgcaga tcaatcaact cacatcgcaa atcatggcct accgtgatcg
    44941 ctcgacgatt taccgtgaaa tgcctcagct tatcagcgat caactttctc tggccggtgc
    45001 cgcgatatat gtggttaacg aaaaagattt aacgctcgat gttttttcca gttacaaata
    45061 tgattttctc cctgaaacag aacacatccc ttttacagaa cctccttttt ttgagacggt
    45121 ttatgacaat caatcggtaa ccatagatgt tcataaactc atcgcattgc aacgggaaaa
    45181 cgaaaggcat gcgcgccgtt tagaggtact tttcgaatct tttcacgcac agaccgtttg
    45241 ttttgttccc attcgcggtc gagcccgcgc agcggatgat tttgagtgtg aaaatcagga
    45301 ttgtgaagcg tttgtaaaaa aagacggaca gtggtggcga aatttgattc atcaatacaa
    45361 ccggcgtgat cttgattacc cttcccgcct tcgtgatgcg agcagatatt tttttaattg
    45421 ttgtttttat gatctcaaag gtgttttagc gctgggtatt ccggatggac gtatgagcga
    45481 tgcgtttcgg cagattattc attcctttac tacgaccgtc ggtcttcggt tagacaatgt
    45541 tcagttgtat gaagatgtac gtgaagccta tatgcttgcc gaaaaacaag ccatcactga
    45601 tgcgatgaca gatgttttta attatcgcta ttttcatcac caactggagc gtgaaatccg
    45661 gcgtgccaaa cgtcattggt caaaactgtc catgatcatg atagacgtag atcatttcaa
    45721 atcatacaat gatcgccacg gtcaccccgc cggcgatgag atattaaaac gacttgccgt
    45781 tgtttttaaa aatatcacac gtaccagcga tatcatcgca cgctacggcg gcgaagaatt
    45841 tgtcatcgta ttaccagaaa cgccaaaggt cgccgctgtg aaatttgccg aaaaattgcg
    45901 cgcggcaatt caagatgaag atttcccgta tgaacaaaca cagcccatgg gtgatattac
    45961 ggtcagtatg ggcgtggcca catttcccga cgaagcgcaa accatagacg aactcataca
    46021 acgtgcggat gaacagcttt accgcgccaa atcaggcgga cgtaatcgtg tagcctatag
    46081 caatacaaca ggatgacagc atgactattt cctttcgcga actcaagaac acaacatcgt
    46141 tatttacgga ttatctttat tcacccgaaa aaacgacttc gtttttttcc gctaacccat
    46201 ccgatcaaaa agcatggtcc gatacgttca gcgaagtttt aaaaaaatat aagacaaacc
    46261 gtgaacttct atcggacatt cttgccgatc agcaacatcg tttccaatcg gcaaaacaag
    46321 ctttggaaaa tgttgcgttg cttcgtcagc cgaataccgt cgcagttgtc accggtcaac
    46381 aaacggggct tttggggggt ccgatatata cggttcttaa agcactaggt acggtaaaat
    46441 attgccgatt gctcaaaaca aaatttcctg actataattt tataccggtg ttttggttgg
    46501 aacttgaaga tcatgatttt gaagaagtac gctcaacaca gcttataaac attcaaaacg
    46561 agcttgtcaa aattttttat gatggcggca tttctgaaaa tcagaacaaa actccggtgc
    46621 accggtttgc gttgacggat gacatatcgc gaataataca agaactgaaa acagcgctca
    46681 atcctaccga tttttcacag gcgttatttg atacgattac aaaatcctac caacccggaa
    46741 aatcttgggc ggaggctttt ggaatgctgt tatcatcatg gcttagtccg atgggattgg
    46801 ttctaatgga tccttcggat gtacgtttta aaggcatagt aaaacctgtt tttgaacgag
    46861 ccctcaaaga atccgaatca ttacatgaag cggttctaaa tcaaagcaat gcgttaaaag
    46921 ttgccggtta tcacgctcaa gttgaaaccg aaccgagtta tgtttttttg acgaactatg
    46981 gcgagaataa actttcgttt aataacaaag ggcacgacaa aatccgtatt acacgggaca
    47041 tctggaaaac atacaaaaat gatattcttc atatactgga gaatgagccg caacgattta
    47101 ttccgaatgt tattctcaga ccattgattc aagatacgtt attaccaacc gttttttatg
    47161 tcgccggtcc tggcgaaatc gcctactttg cacagtttcg ggatttgtac actcacatgg
    47221 atatggcatc gccggtcata attcccaggc cattttttac tttagttgag aaaaaaatca
    47281 aaaaagtgct cgataaatat caaataacga tagcggacat catcaatgac ggcgaagcgg
    47341 tgatcgaacg aattaatcaa caatccaatg tttcgcttga accggcgctt ctgaggttta
    47401 tgaacgaagt gcaagacggt atgaaaaacc ttgaaacgca attgacggcg atagatgctt
    47461 cactcaaagg tgcgtctgct acggcgatgg ataagatgca acaagcgatg caggtgctta
    47521 cacaaaaaac acgcgatgcc gaaaaacgcg ccgctctgac gacccatggc caactgacga
    47581 aagccatgct gcatattcgt cccaatgata actttcagga gcgtgagctt tcgattttat
    47641 attatttgaa taaatacggc tttactttgt ttgatacgat cagtgagcaa atcactttgg
    47701 actcttggga tcatcgtttg atcgaactgt aaggtcaaac ctttttgagc tccaaatcct
    47761 ttgcttttat tatgagcatt gtctaatttt tcactgttcc attctgacgt ttcatccggt
    47821 gttgctttta caaacgcatc ggtttcacgc ctatactcac aacctttctt tggagatacc
    47881 gacatggata acggttcacc cagaaatctg gcggatgtat ttcaccagag tgcgcgtcgt
    47941 tttggtcata aaacgctgta tatgagcaaa cgtgatggca agtaccgcgc tttgacgtat
    48001 ggacaggtgc acgaaatagt ctgcaatctg gcgctgggta tggcatcact gggaattaag
    48061 aaagatgata aagtagccat tctctgtccg actcaggaag agtgggctat gtccgatttt
    48121 gcgattttgt cactcggcgc cgtaacggtt ccgatttacc ccaatcttcc tacgccgcag
    48181 gtcgaataca tcctgcaaaa ttcggatgca cgtttagtgg ttgcatcgga tcgtgagcag
    48241 cttgcgaaaa tcattgagtg caaaaatcag tgcccgatgt tgaataacgc cgtgcttata
    48301 actcacgaag ataaacacaa agactacgtt ttgttttttt cagagcttca ggagttagga
    48361 aaaaaatttg gcactgaaaa ccccggatat atcgaaaaac aaatcgacag catagatccc
    48421 aaaggattgg caacgatcat ttatacatcc ggtacaacgg gtatgcccaa aggtgtgatg
    48481 ctctcgcaca ataatatttt gagtaatagt ctagcggctg ttaaagtggc taccgtcggc
    48541 gaaaccgata ccatgttgtc gtttttacca ctcgcgcaca gttttgaacg tatggccgga
    48601 cactttatgc cggtgattcg cggttgcgcc attgcttatg cggaaagcat cgaaaaggtg
    48661 ccgcaaaaca tgggagaaat caatccgacc gtaatgaccg ccgtacctcg gttatacgaa
    48721 aaaatgtacg accgtgtaaa agagggacta tctacggcgc cccctattcg tcgcaaaatt
    48781 tttgaatggg cgctcggtgt aggtaaatcg gcttatgaaa ccggtgaacg cggattcaaa
    48841 tacaagatcg cggaaaaact tgttttttca aagctgcatc atcggcttgg cgggcgctta
    48901 cgattttttg tgtcgggcgg cgctcctttg gccaaagaaa ttgctgagtt ttttgcttat
    48961 atgggcgtga tgatcctcga agggtacggg ttaaccgaaa cttctccggt aatcaccgtc
    49021 aatcgtcctg aaaaagtaaa atttggagcc gtcggtccgg ctattgacgg tgtcgaagtg
    49081 cgtatcgcac aggatggcga agtattgaca cgcgggcctc acgtcatgat ggggtattac
    49141 aagaacgaag aagccacacg tgaaatgata gactcggatg gatggttgca caccggcgat
    49201 atcggatttc ttgactctga taatcattta cacataacgg atcgtaagaa aaacatcatc
    49261 gtaacatccg gcggaaaaaa cattgcacca cagccaattg aaaacacgtt gatcaccatt
    49321 ccttttgtag agcaagcgct tgtcatcggc gatcgccgta attttatctc ggcgttgatc
    49381 gttcccaaac gcgaacgcct ccgcgactgg gcgatgcagc acggtatcaa gtttgacagc
    49441 gaagaagaat tgctggaaaa caaagaagta tacgccatga tagataaatt gatccaagaa
    49501 gcgcaagata aagccggatt tgcgcggtat gaaaaagtgc ggaagtttgc attattaccc
    49561 aaagcatttt cgatagaaag cggtgagctt actcccaaac tttcgatcaa acgccatgtc
    49621 gttactaaaa attttaaaga tatgattgat acgatataca accgcgaaac agaacgtatg
    49681 acggaaggcg atagtttgac atgatagata ttaagcatat cctgaccgtc ggtgttgacg
    49741 atcaggtcaa aaccatttat gaagattata tcgcgcagct taccgaacgc atcggtataa
    49801 gcgatgcgaa tgcaaaaccg gctttcaaag cgttgatgaa cattctcatc acgcttgagg
    49861 aaagcggaaa tatccgcaaa gggtacgatt cgttgccttc gctttttatc acccaacgcg
    49921 tgctgtatat gaaggtgttt gatctcatgt tgcagcatgg gcatgccgtt tccgatgtgg
    49981 atgaattttg gtttcgcggt aaagaaattg agttagcatc gccatcccac tttattgacg
    50041 atcaagttca ggtagcgcgt tccatttttt cggtgttcca accgtttctt gatgcggatg
    50101 acgacgttga tcgttttatg cgtgtgattt cactctatgc cgcatttatc agtaaaaaga
    50161 acgatgtcgg tgcgatggtc aatctgatgc atcactttgc gcattttgca gataaaatat
    50221 atctcgccgg ttataaaaaa gtgcatgacg gatggggcgg taaattagat ttggataccg
    50281 atgtgtatcc tgtttttttt aagtctatta ttcgtgatgc gattgaaaca tatggctttt
    50341 cagactacgt cgtagatttt ttatatgatc gtctcaaaga agtgggcgaa tccgaaaccg
    50401 tactgcccga tctttatgat tttaccaagt cgcttcaggc caatgcggaa ttgacgaaag
    50461 aaatacaaga cgccgataaa tctttaaaaa cggaaattca aaaaacgttg cagatcgttc
    50521 aacagcaaaa aggcgaatcg ttgagcgaaa acgcacaaaa cgccattgtg cgtgaactta
    50581 ccgcgtacga agagcttgag ggattgatct actattattt tgacgaacgt cgtcttttca
    50641 aaataatgac cgatacgaaa acggtatcgc tttttgaaaa aacgttggac attgtgcgct
    50701 cgtattcacc gaaaatgaaa cattatgtaa tgtacgcgtt cggaaaactg ctt
//
LOCUS       DAOQNQ010000002        42015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_277_length_42015_cov_6.169828, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000002 DAOQNQ010000000
VERSION     DAOQNQ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 42015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42015
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_277_length_42015_cov_6.169828"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..778)
                     /locus_tag="PK754_00215"
     CDS             complement(194..778)
                     /locus_tag="PK754_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNB07956.1"
                     /translation="MNRMMVLAAALLINISTFAQSNWGVDVSHSNVLFTVSHLVVSDV
                     QGYFKEFDATLGYSKEDFSDATATMTIKVNSINTGIEKRDAHLKSDDFFAADKFADIT
                     FASKSFKKTGKNTFKITGELTMRGTTKTVELDAVYRGEIKDPWGNTKSGWKINGSVNR
                     FDYGLKWNAAMETGGLVVGENVDITINLEMVKKK"
     gene            1197..3656
                     /locus_tag="PK754_00220"
     CDS             1197..3656
                     /locus_tag="PK754_00220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HNB07957.1"
                     /translation="MTETAVQSQQTCIHCGDICDNEVVRLDGKTFCCQGCQWVYQILH
                     EHDLGEYYDLQNGVRSLPKAKTNVKYEFLDIPEVRKSFIQFEHDHRVRVTLFLPQIHC
                     SACIWLLEHLHKMESGIQSVRVDFLRKEAYILFDDRLTSLRQIAERLASIGYEPDFSR
                     HDKSGATAPAVERHLYIKLGIAGFCFGNIMLLALPDYLAGGQIEAPLRDYFRYLSIVV
                     ALPLLYAVSGFFSSTFNALKEKTITMDVPISIGITTLIVRSLWDITHGFGSGYLDSLA
                     GLTFFLLIGKIFQKKTFYTLSFERNLKSYFPLAVQKKTDDSAVVIPIEQVQVGDLLIV
                     RHQELIVADSVLINGIATIDYSFVTGESIPVSVMQGDKIFAGGRQTGSAIEVEVVKEV
                     SQSYLQQLWNQEAFTGSSESRIQNISNTVAKYFMVAVLTIAVITGLYWYVTDPAMVID
                     TVTSVLIVSCGCGLPLSIPFTFGTALRVLSEQQFYIKNYHVVENLAVINTIVFDKTGT
                     LTKSQGSSVSFEGSSLTKEQQAIIAAVVRNSTHPLSRAVYDYLDHVETYVVDRYEEIS
                     GKGITATVHGHHVKIGSLAWLEGLTLAENSPFYSDAQRSQMHIVIDGSLLGCFTILSE
                     FREGTEYLSKVIPKDYQLFVLSGDSDREKNRLEQLFTNRAVMLFRQLPIDKLQFIRQQ
                     QVSGRRVLMIGDGLNDAGALKQSDVGVAIAENTAAFTPSSDAIMDVKALPQLARFLIF
                     SKQTKKTVYAAFGLTLLYNLVAVTLAVQGLLAPVAAAVIMPISAISVVAFTVIAVRWI
                     ARKNQLLKTVSDVPIPSLSNA"
     gene            3653..3835
                     /gene="ccoS"
                     /locus_tag="PK754_00225"
     CDS             3653..3835
                     /gene="ccoS"
                     /locus_tag="PK754_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HNB07958.1"
                     /translation="MNILVVLIAVSLMLAFGFLAAFLWAMNRGQYEDTHTPAMRMLLD
                     ENQNHITSNPSSSTEN"
     gene            3841..5994
                     /gene="ccoN"
                     /locus_tag="PK754_00230"
     CDS             3841..5994
                     /gene="ccoN"
                     /locus_tag="PK754_00230"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009282386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HNB07959.1"
                     /translation="MEKTSVAHEPQVTGRLEQFEYDNDIVRKFAIASVVFGIVGMLVG
                     VIIAIQIYMPALNLDIPWLTFGRIRPLHTNAVIFAFVGNGIFMGLYYSLQRLCKARLF
                     SDTLSNIHFWGWQLIIVSAAITLPLGITSGKEYAELEWPIDIAIALVWIVFGINMFGT
                     IIKRRERHMYVAIWFYIATWVTVTMLHVVNSLAIPAGWFKSYSVFAGVQDALVQWWYG
                     HNAVAFFLTTPYLGLMYYFMPKAVNRPVYSYRLSIIHFWALIFLYIWAGPHHLLYSAL
                     PDWAQSLGSVFSIMLIAPSWGGMINGLLTLRGVWDKVREEPVIKFMVVAVTAYGMATF
                     EGPMLSIKNVNALSHFTDWTIAHVHIGALGWNGFLTFGILYWLVPKMWNTTLYSKKLA
                     NAHFWLGFLGIVFYAVPMYWAGITQGLMWKQFTPDGTLQYFNFLETVTQIIPMYVIRS
                     IGGTMFFASMFIMAYNLIKTAKQGQFIANEKAEAPALTKEYVSHGQSYYHRLIERKPL
                     PFIILTLIAVAIGGVLEIVPTFAVESNIPTIASVKPYSPLELQGRDIYIREGCYTCHS
                     QLVRPFRSETERYGEYSKAGEYVYDHPFQWGSKRTGPDLHRVGGKYTNSWHYSHMMDP
                     RAISPGSIMPAYPWLFENSLDISTTEKKISAMQIVGVPYEKGYEKVANNDLKAQATAV
                     AGDLTQNGFPTTPDKEIVALIAYLQRLGTDIKVKQ"
     gene            5995..6198
                     /locus_tag="PK754_00235"
     CDS             5995..6198
                     /locus_tag="PK754_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019598022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HNB07960.1"
                     /translation="MLRRILENAGGLEIWPTIALVIFFVFFVGLIAWLVRIDKRHLHH
                     METMPLDGEEIAVSNNGYKHGGK"
     gene            6200..6865
                     /locus_tag="PK754_00240"
     CDS             6200..6865
                     /locus_tag="PK754_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020604279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HNB07961.1"
                     /translation="MAEVKDELLDHDYDGIREYDNPLPPWWVNMFYITIVWATIYLIF
                     YHVLEIGDRSVDEYRKEFDNTWTKEMDPNYQKVDMLGTGYKGPYYYKPSDPTNKGVVA
                     FMPAAEKPKEEELTKEFAALTDAVSLGKGKEIFTVNCVPCHGKLGEGGIGPNLTDDYW
                     IHGAGINNVVRTAWKGVPEKGMISWKQLGEKNVLLVASYILTLRGTNPPNAKAPQGDL
                     VKQ"
     gene            6890..8320
                     /gene="ccoG"
                     /locus_tag="PK754_00245"
     CDS             6890..8320
                     /gene="ccoG"
                     /locus_tag="PK754_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HNB07962.1"
                     /translation="MAEVKEELAQIKNADGETYRDRIGIIDKKGARIWIYPKLVAGVF
                     HYWRVIVSIILLFVLFGVPFIKVDEHPFIMFNILERKFILFGLAFWPQDFYLFALTFL
                     SIVIFIVLFTSVFGRIWCGWACPQTIFMEMVFRKIEFWIEGDGPKQYALDQAPWNADK
                     ILKKTFKHVIFFAISWIIGNTLLAYIIGVDELFKIITDPIGMHVNGFTAMMIFSLIFY
                     GIFSRFREQACIYVCPYGRLQSVLLDKNSIVVMYDFQRGEPRGKLKKGNTGGESLGDC
                     VDCKLCVQVCPTGIDIRNGTQMECVNCTACMDACDSVMEKIKRPKNLIRYGSYNLISQ
                     KLHFKITPRIVLYSAVLTVLITVTSIFLFKRKDIQTTILRAKGPIFQLMENGDIRNVY
                     TAKVLNKTFDAMQIEFKLEGVEGNIALIGTHELRLHPDELVESTFLVDIPRNSIHTMN
                     TKIKIGVYKDGMKVSTEETSFVSPMQ"
     gene            8332..8778
                     /locus_tag="PK754_00250"
     CDS             8332..8778
                     /locus_tag="PK754_00250"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNB07963.1"
                     /translation="MSLFRNWGFRITVLYVSFALLILGMVFVSSISREDLVSTNYYDN
                     EIAYEKRIQAIKRTKMLEQKPAWNISGRVLTMIFPEQISQITGGTLTLYRASDADRDI
                     VVPLKLDSQRSQAIDLQKAIPGMWRAKLEWKSENNDYYLEDVIYIE"
     gene            8797..9513
                     /locus_tag="PK754_00255"
     CDS             8797..9513
                     /locus_tag="PK754_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNB07964.1"
                     /translation="MFEIYSALLVGVLGSFHCIGMCGPLALAIPQHDRRWFMLVRSAL
                     LYNGGRAFTYAMMGLVVGAIGATLQTVGLQQGLSIGLGVAILLALLLPNSIKQKIWPD
                     SSLQGGWAAMRRWFGQLLRDPNPTSLFTLGLLNGFLPCGLVYIALGGAMATGHAWEGA
                     LYMALFGLGTLPAMTAVAIFGQFITTTTRQKITKLIPVVVGGVAVLLILRGLSLGIPY
                     ISPDMHAVAAGSGESCCAKH"
     gene            9500..10600
                     /gene="thiH"
                     /locus_tag="PK754_00260"
     CDS             9500..10600
                     /gene="thiH"
                     /locus_tag="PK754_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HNB07965.1"
                     /translation="MPNINLNMSFYTRWQSYRWPVVENLLGTRTKKSVENILADASDF
                     KFPEDVITLLSPASDTYRHVIAQQAAQQIQRPIHLYTPLYLSNYCHNSCTYCGFRVEN
                     KIQRVVLDQTQIEKELQAIAATGVRRVLLVTGEERRFAGVTYIAEAVRIAKTLFDHVR
                     IEVQPLNTDDYALLREAGCEGVYIYQETYHETNYRLYHPRGMKSHFRHRLETPDRIAM
                     SGISKIGLGVLLGLENWRVDAFFLAMHAMYLKEAYPKLQIAISFPRIRPAVGVHNKVS
                     DVFESDLHHLVAVFRWINPDWDIAMSTRESPDFRNRTLKLGVTSISVGSKTQPGGYLK
                     NDTAELEQFAVHDNRSAETMRSFLEKNVRMYD"
     gene            10845..11261
                     /gene="rplM"
                     /locus_tag="PK754_00265"
     CDS             10845..11261
                     /gene="rplM"
                     /locus_tag="PK754_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019108417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNB07966.1"
                     /translation="MPKADQIKQKWFVVDAADQTLGRFASRVASILKGKNKAYYAPHM
                     DCGDFVVVVNADKVRLTGKKTKIKEYIHNTMYPGGQRITKFEDMIRTKPERVVYLAVH
                     GMLPKNTLGAKMIKKLKIYAGEKHPHSAQNPETLKF"
     gene            11281..11670
                     /gene="rpsI"
                     /locus_tag="PK754_00270"
     CDS             11281..11670
                     /gene="rpsI"
                     /locus_tag="PK754_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNB07967.1"
                     /translation="MSAPLAAVGRRKTATARVRLVPGNGKVTINHRDIKDYFPRGSHQ
                     IHVTEPLTVVEAGSKYDVIALTRGGGPSGQAGAIRLGIARALVKIDEEMKKALKPIGA
                     MSRDPRMKERRKYGLAKARKRYQFSKR"
     gene            11877..12719
                     /gene="rpsB"
                     /locus_tag="PK754_00275"
     CDS             11877..12719
                     /gene="rpsB"
                     /locus_tag="PK754_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNB07968.1"
                     /translation="MSEVQVSFDPKKKTEELLAAGVHFGHLTRRWNPKMKPYIFMEKN
                     GIHILDLRKTVHSLEGACKALAKIVANGERVLFVGTKKQAKEIVQNEAKRANSFFVTE
                     RWLGGMLTNFSTIRKSIKHMKNIEKKEIDGTFEKITKKERLMLEREKAKLKIVLGGIE
                     DMKQLPGAVFVVDVKKEHIAVKEANVLGIPVFAIVDTNCDPDTVQHVIPGNDDATKSI
                     EAITKTIADAVAEANQHAVAAAKEKEIAEVDEERVKDETRMKYEETNFNKKRLGKKEV
                     KKED"
     gene            12770..13666
                     /gene="tsf"
                     /locus_tag="PK754_00280"
     CDS             12770..13666
                     /gene="tsf"
                     /locus_tag="PK754_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNB07969.1"
                     /translation="MSAVEISASAVMKLREETGAGMMECKKALTEAGGDAAKAVEILR
                     ERGLKKSAEKQSRVANEGTVLSSVNDAHNFAVMVEINCETDFVAKNDDFVKFSNNVLA
                     QVRDSKPANLDAFLSAPAAFAAGQTIADAVTGLTAKIGEKIAVKRIAVIENNQGVIAG
                     YIHPGNKLGVLLQLNVEGFAATMTADAYSVAYDIAMQTAAMNPTYIHREEVPADVVEK
                     ESAILREQLKNDPKNANKPDNVLTNIIKGRLDKFYQEVCLVDQAFVKDPNKMIKDLVA
                     DLSKKLGKPVTIRRFERFRLGE"
     gene            13790..14506
                     /gene="pyrH"
                     /locus_tag="PK754_00285"
     CDS             13790..14506
                     /gene="pyrH"
                     /locus_tag="PK754_00285"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNB07970.1"
                     /translation="MPIFKRILLKLSGEALMGEQGYGIDTKVLEQIAKEIKDVKALGV
                     EIAIVIGGGNIFRGLAASAKGMDRVSADHMGMLATVMNSLALQDALEKQGLFTRILSA
                     IKMEQVCEPFIRRRAIRHLEKGRIVIFAAGTGNPYFTTDTAASLRAIEVEADVILKGT
                     RVDGVYDSDPEKNKEAFRFEKITYLDVVKKGLNVMDMTAVTLCMDNKLPIVVFNLKTQ
                     GNLRRLVLGENVGTRVQQEQ"
     gene            14545..15108
                     /gene="frr"
                     /locus_tag="PK754_00290"
     CDS             14545..15108
                     /gene="frr"
                     /locus_tag="PK754_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014258400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNB07971.1"
                     /translation="MYDLNKQYHETEEKMKKSLETVRNELAKIRSGRATTTLLDGVKV
                     DYYGTPTPLNQVGNVSAPEARLLTVQPWEKGMVPAIEKAIREANLGLNPQNDGALIRI
                     PIPPLNEERRKELVKLGKKYAEEGKVAVRNVRRDANEHFKKAEKDHKISEDDSKDAQD
                     KVQKMTDNYIKKIDEMLVIKEKEIMEV"
     gene            complement(15201..16349)
                     /locus_tag="PK754_00295"
     CDS             complement(15201..16349)
                     /locus_tag="PK754_00295"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB07972.1"
                     /translation="MKLFFSFDVLTLLFRFQTHNLFFEANMAKRVSWFFLLAIVTMVL
                     CSCRTLYHTETIVFYNNQIRFSGTFYKPLTSAPYPVIVIAHGSGTADRNSFFYKPYGE
                     YFSKHGIAVFIFDKRGVGDSEGVYDDSPDFQLLASDLKAAFQSLSSRNDIDKNKIGIV
                     GISQAAWVLPITAQNLDGLSFVVCTSCPSVPPYESDLFQKGRELIEAGYSQNDIDDIL
                     NYNRAVTEYVATFVGREKVLQMKHRFKDRQWFRDFNYNPDLSPEDTLKQPQYDHYRNS
                     TFDPMPFWNQVKTPVLFVYGSKDSHIPVVQSIKRLEDSIEADQVNYRIKTYRNAGHLI
                     QSVEDSVESLKFSLFRFIKGRPQPIPEYLEFVRRWVLAPSQVDADTVL"
     gene            16769..17992
                     /locus_tag="PK754_00300"
     CDS             16769..17992
                     /locus_tag="PK754_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB07973.1"
                     /translation="MLVNAKIRTIIQWEFTQRVISKGFLFSVIFIPAIILAFTLLPVF
                     FANSNGNRIFSVGVFDATRRLGEDLSSKLQDLTYSEIQLVPLASSNVEEALAEGKRLA
                     KEDGIDAVLVLTADMFTQRKIEIYTDYRTENAVQIELLKQAVAGLLMEYRMRGKNFTH
                     EEIQNITAPLQVDTYKLGSDHAASLQKYMTGIILVMMLYFAIANSAGSFMRGLSEEKN
                     NRVIEVLVASASPREIMAGKVIGLGLVGLLQIAVWTTLAVMLGSRKEIAALSPELLLF
                     FILYFVMGYMFFAGINALIGAIFASDQDIQPLQAVISMLGVVPVALAFWVIEVPSDIA
                     IRILSYVPFLTPTMMILRIIVSNPPWWEIGLTFITLFVGVVLVFRMAGRLFEISLRMH
                     GKKISFREMVRWARA"
     gene            17977..18588
                     /locus_tag="PK754_00305"
     CDS             17977..18588
                     /locus_tag="PK754_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNB07974.1"
                     /translation="MGSGIKIKVTTLAIKNMVCDRCIRVVREELVNIGVNVRHVELGK
                     AIVEGNLTLPMQNKLRTVLTQNGFELLEDKRAKIVEEIKNQIVIYVQKNDVENKNIKL
                     SVLLSRTIGRDYSYLSGLFSSVEGITIERFLILQKIEKVKEWLAYDDRTLSEISYKLG
                     YSSVPHLSKQFKQITGLTPTEFKQQNDHRRIPLDKIKIIHRIT"
     gene            18601..19362
                     /locus_tag="PK754_00310"
     CDS             18601..19362
                     /locus_tag="PK754_00310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB07975.1"
                     /translation="MRQKYYLVSGAGTGIGLAIAKKLAERADHSVILCGRRQDVLSEA
                     MHQLSNTRNHSILVMDIHNPGTIKKAAAQLAIPYLDGIISNAGIGGENQYGDEDRWDA
                     IIATNLTGTYHFVNNFLPIVRKSPNTYRHIVVVSSILARLGVARYSAYCASKAGLLGL
                     MRSWAVELAPEKILVNAVCPGWVNTKMAQDGMEGIAKNIGVTTEEFYRMAMESVPLRK
                     MSEPEEIADLTHYLLKQRSITGQTLDINNGAIMNS"
     gene            19433..19951
                     /locus_tag="PK754_00315"
     CDS             19433..19951
                     /locus_tag="PK754_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNB07976.1"
                     /translation="MKYKVTTEQKEYDIEVVPQGASFKVRVGKSPDIEELDVDFHTVG
                     QGSVHSMLVNDKSYRVIIDKKMHKHAVYTRRHRFEFNVEDERTFLMRSLIAGSGAKAV
                     GEVKAPIPGLVSKLLLQEGDTVNQGQGVLILEAMKMENEIKAPMAGKIKKFHVQGGKN
                     VEKGQPLFVVEP"
     gene            19978..20688
                     /locus_tag="PK754_00320"
     CDS             19978..20688
                     /locus_tag="PK754_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNB07977.1"
                     /translation="MNKKFELPLCDQCHSRVHSVFGDLKDSDVKSLNENKCGNIYKKG
                     QVLFYEGNKPTGLFCVNKGKIKTYRINADGKEQIIRLAKEGDVLGYSSLISGDLYSSS
                     AAVIEDAIICYIPRNVFLNLLDSNTTFSMKMIRLLSHDLKSSEDRVVNLAQNTVRERL
                     AETLLMLEEFGGTENDGKTLRTSLSREEIANIVGTATETTIRLLSEFRSDGLIELEGK
                     RIKIVDRGLLLKAANVED"
     gene            21412..22545
                     /locus_tag="PK754_00325"
     CDS             21412..22545
                     /locus_tag="PK754_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07978.1"
                     /translation="MELKAVLSRFRQMVGLSLIVAVFSFTTVVAQGTTNGVTITPEPL
                     TPFTFTSFPLSLLGIGGSDANLSSFNNFGLRFENNSGSVKIIRMGIDLKTSQGSIFNK
                     KYLVTLTIPSGVPIFVGVPQILNGSFGSIKASNRQSISISDDFMKQIKGGFPSGLFTF
                     DIKAGLPNVNNYDGLDYMGSLQIQVLGNSPVEIISPSFGFESIDGLPQFAWSAQGATK
                     FLLTIARKKGSQSDEGAIKSVVQRAVIELDNVFSYSLPSGGPSTSKENNLNCNPGLEP
                     GDYVFQVTAVIQDNFTGKSRNVDSPIRAFTVKETISSTAGLNTEEILGLLNDAAKGAV
                     NFKDQLKGFKAVSISIKGNTATIDDLRTAVQNLPEAVKFEIKP"
     gene            22589..23266
                     /locus_tag="PK754_00330"
     CDS             22589..23266
                     /locus_tag="PK754_00330"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNB07979.1"
                     /translation="MKSFMIKMTALLACVATVITATLYLAEANDGKAVAIVVKSTGKA
                     QAKQGNKWVDVTKGSRLFSGDKVVTKDDGFAAVMFLDDKSIIKLKPNSNLEVKGQYEG
                     KSISKNIVMDVGELFVKVSKQKGSFQVATPTSVASVKGTEFWVIENTNGTKVIGIEGL
                     IEVLNKASNKKQDVSAGNTANSDKSGNLNVDTTKENEIPEDKDLEEIIIKFRDNDGKE
                     KEVKIKY"
     gene            23464..27915
                     /locus_tag="PK754_00335"
     CDS             23464..27915
                     /locus_tag="PK754_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNB07980.1"
                     /translation="MARWIRLLLMCCIVPIQAQSDWINLGPYGGEYETMAIDQKKPNN
                     LYIGTRNGQIYRSTNSGQTWSFLSAIDGTITKLVVDSSNGNNIFVGVHDRNNFNHVFY
                     SKDAGLTWTGNYALNLYNEEIKDFVINPANNTQMHLVTVNRSIPSINGGKSFVGSGPY
                     FPDGFHSFAYDPHDPTNVMYAAGHDNSFDRDIFQGTRNAWSNVHTYQYMETGWRIGKV
                     HVENTNIAAVYVLIGNEDSTKIMRSLDYGATWAIVFSDDVAMYQGGFKIGQYAWLHRS
                     KGNNEHIVATQNGILKTNNGGLSWYRMKDGLNNNLSLVRGGTVVRSPQDTSIFYCNYS
                     KKPARLSITPYGYVANVNQSLGFTAQEIKKVHYSQNNYVWAETQTRDLIYTSNYGQDF
                     YETGIMLPPGDEIIGGNGFFVTKRGSYLQSAGAVLGAITFPSGTDSVIAVIGSEVFSS
                     GLYDNYEFGITQNSTGGTSSGFFIARYDFSTNNVISYIPTLPDIALTSPITSFSFSFR
                     SYQRDTVDIYVAASTGIATTYFANGTFGSWKTIKLFDGSFTIKKIEFRETEAFIWAKE
                     PGGDRLFIGHRDDMSSDFSIEEISGSFPRDEFGQSEIHDIHAGVDGYYMIRFRSDFGA
                     NGAEYTRPLYKAGFGKEPRWTEITTPDLSKFNIQNVLTEYRFQENAVVIYAAANNGIW
                     TMTQTASISIDTLVIFSSTSALLDSSIAELKYANTGTAFSYIERFEITDDAENSFSIA
                     DKNFADGDILFLDIRSETSFPIKFKPVSKGLKTAKLKSYYTVRASNGSDSTIIATTRL
                     VGIGKTSQIGTNLKNDTLDVKGALVNASKTTNFQLFNSGEDDMYIEDIYLSKNDVFAI
                     QSINESDSTSFIVAPGRVLPVILKFEPTEGKSYTDTLIIESTAFKANNTPNPKHRIVV
                     KGVGSSIQVSNIDNLSASLNQALTIEVSLSQIDALEVTGLLRYRKNGEGSSGYKNKTL
                     TPTVTQSGNSSRFTTTIPQEDITQNGLEFYVEFTSSSSVSPIQYPENGEFEPQVVSVT
                     IPNPGLSSSGYVSLPGGTDKKAFQLMSFPLNLTNKTVGGAFTPSNMGEAGVKGDWQVY
                     RYDNDLSKFISSSDPDITFGNIESGKSYLLVSRKPKTINSGAGKTVKKEDAHVTIQPG
                     WNMIANPYAFSINWSDVANFNDNVELTNIVVLQNGRFKYVDEVDVYNLKLEPWKGYMY
                     YSPEENGTFTINYPAINSDNASALQKKEIRTTVDKSGYRFLLTLHDDGEDIVFEAGTA
                     EDAQDGADGRDKRALPMLMKDEVSLHWLQADRKLQTDMRAVSPEGHVYTFEVATGVDR
                     RTVSLSYSATNLPEGFSVRMVEVTTGMVQTGETLNLMVQNGKPKMYKLLIGTEAFIAK
                     AQSEIIPSQFSLAQNYPNPFNPSTTLRFGLPEASSVKITIYNMLGQQVRTLVKQVMPA
                     ASHALLWDGTNDFGQRVASGVYVYRLEATGLGSNKVFSQTRKMTLIK"
     gene            27936..28592
                     /locus_tag="PK754_00340"
     CDS             27936..28592
                     /locus_tag="PK754_00340"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNB07981.1"
                     /translation="MKRFKKIIAVLLSAAFWNIQAALLFSHSIHAQQGTRPLVAVLDL
                     QVTGGILKSEAQSLSDRLRGEMQGLKKFDIIERSQMDALLKEQDFAMSDMTEANAAKA
                     GKLLSVEQIVVGSIGRVGRSYTVDVRLIDVVSGRVITASQQDYNGPTEGLTQVIRNVA
                     RQFAGLPPLKVSVASNTKWWVMGSLLAVGGGVAAYMMLNKKESGSSGPPAFSDPPALP
                     "
     gene            complement(28668..29741)
                     /locus_tag="PK754_00345"
     CDS             complement(28668..29741)
                     /locus_tag="PK754_00345"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB07982.1"
                     /translation="MIWTIIYTLIFGQGIFLAIVLFFNKKGSVLANKSLALLMLIFSS
                     AVLEQLIRISGLFSALPHMLFISVPFWYALAPAYYLYAKNLVEKQNQFVIKDAWHALP
                     FLGVILLLSPYYLWDVQKKIAYIIDPSQFNDFALESIILNVVMVLQNVMYLTMAIRLL
                     SQNEAYSKLNRIIRFLFSIMMGYVVFNFINAIYFLFTGETLVHWGNWSIPIFATIIYT
                     IAFLSFVSPETIFFTLWPRNNNSLSKERKHEVLNQLYKLMEQDKIYREPDLRYTEVAA
                     RLGISARCLSALLSEDIGMTFNVFVNTYRVKDAQNQLRERYCEETLLAIALDSGFSGK
                     SSFNRIFKEQTGLTPSEFVYQSN"
     gene            30044..30769
                     /locus_tag="PK754_00350"
     CDS             30044..30769
                     /locus_tag="PK754_00350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB07983.1"
                     /translation="MYSKDYFESDFRCGHEDASAFNKKTDPVLPDWTEQVHLQPESSV
                     LEIGCATGYTLKAFHDAGHHVKGIEISEDAARHARDVYRLDVDTMAVHQAQFSSEKFD
                     LIYLLDVFEHLTDPVLLLKQAYQWLKPGGTLVIVIPTQTNTLFSRFGMTLFSLAGKRT
                     RIHMPPYHLFEYRPSSVRRLLLNHGFSKIDMKPVIMKPGEIGLRSSVVQNTAKYVFQC
                     LNYPLTKLSGRFGDRLTIIAVKS"
     gene            30844..31326
                     /locus_tag="PK754_00355"
     CDS             30844..31326
                     /locus_tag="PK754_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNB07984.1"
                     /translation="MKSLITLKVNGDLYEIAVKSNRTLLEALRYDCHLTGSKQGCDMG
                     DCGACTVLIDNVPIQACITLAVECVGKEIITVEGIAKGTEAHPLQTAWNNQGASQCGY
                     CTPGFMVVAKWLLEKNPNPSDEEIKEALSGNICRCTGYTKIITAMKEAAAIMQKEKAV
                     "
     gene            31386..33788
                     /locus_tag="PK754_00360"
     CDS             31386..33788
                     /locus_tag="PK754_00360"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNB07985.1"
                     /translation="MIKPNPKKNQFSIIGKPNPKIEGLEKANGSALYTDDLMLPNMLI
                     GKILRSPHPHALIKKIDTSKAEALPGVKAVMIGTELTEKYGVLPASQDETVLAVDKVC
                     YIGDGVAAVAALDEETAEKACELIEVEYEVLKPCLTIEDGFREDLPKIHPHTKQANVM
                     KEVHQEFGDVEEGFKEADLVMENEYFFEGNTHLPLEPHCALAQYGSDGKLTIWSSTQT
                     PHYLHVALAKVLQMKRSHIRVIKPHIGAGYGGKSEPFALEFCAAWLSKKTGRPVKFLY
                     TREEVFYAHRGRQPMKMILKTGVKKDGSITAIHMRTFLDGGSFGSYGLVTTYYSGVFL
                     TLPYKVPRYKFDAYRIYTNKPAGGPKRGHGAVQPRFAMECQLDKIAEKLGLDPVEMRL
                     KNSIEPNTMTINSFRVTSCGIKECLTSTRSRSEFDKKYASYKKQNSKVIRKGIGIAAS
                     TYISGAGKSIIWNDMPHSGVQIKVDRGGGVTVFCGASDIGQGSDSMLAYITAEVLGVE
                     ISDVHVCQTDTHLTPVDLGSYSSRVTFMAGNACIQAAERIKEMIFEIASRKLGVPVTD
                     LDNGKRLIFSKSDPNKFMDFVEAANMAESAYGTIGATGSYKPPKIGGSYKGAGAGPSP
                     SYTFSTHIAEVSVDVETGAVTVEKIWSAHDCGRALNPVIVEGQIEGSVYMGVGEALFE
                     EHIFHKNGLHRNPSILEYKVPTILDTPEIESIIVESIDPEGPFGAKEAGEGPLHAAIP
                     AIANAIYNAIGVRLDTTPFTPDKILKALEEKELAEKGGNGHSKKLRDIGAKKLVSHIA
                     TT"
     gene            33970..34428
                     /locus_tag="PK754_00365"
     CDS             33970..34428
                     /locus_tag="PK754_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNB07986.1"
                     /translation="MRDYVLFAVLAGLAWGLGGYFEKSGLRAMGIPPIAGITLRTGVA
                     FILLALLSIPAWKQIANVNDTTAWVMIIIGGGVVAGSLGMWSFYKSLSTTENLGVTLA
                     LAFSISPIAGTVMGLFKGNQPMNWQTGIGLVTIIIGIIILQTSHAPTAKP"
     gene            34474..36705
                     /locus_tag="PK754_00370"
     CDS             34474..36705
                     /locus_tag="PK754_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNB07987.1"
                     /translation="MKTFILSMFLFLQWPLVSQDSTMLSLERIFDSDELQPRFFGPAR
                     WLKNGKGYTTLEVSDRVEGKDINKYDAATGTRTVLIGAEKLIPQGQTRPLKISDYFWS
                     SDDSRLLIFTNTRKVWRYNTRGDYWVLDIGAGKLHKLGGADAKTSTLMFAKFSPDGQQ
                     VAYVRENNIYTENLLTGKITQLTFDGSDVIINGTFDWVYEEEFGCRDGFRWSPDSRAV
                     AYWQLNADGIGVFYMINNTDSVYSKVIPVQYPKAGYPNSSCRVGVVSASGGTTVWMQT
                     PGDPRDNYIPRMEWAGTSEAIVMRHLNRLQNTLQIMLGDVKSGIVQTIATDKDSAWID
                     IDDEFKVLDGGKGFLWISERDGWRHVYKTSRDGKKTELLTPGNFDVIKVILAQEKENM
                     LYYTASPENATQRFLYRMKMDGKGKAERITTETGGWHDYQISPDAKYAIHKYSSMQLP
                     QTVEVVDLPKHKTLRALEDNLALRQRLAALKINVPEFFKIEAGNELMLDGWMLKPASF
                     DATKKYPLFVFVYGEPAEQTVVDHWGDTDYLWHQMLAQQGYIVISLDPRGTPAPRGRA
                     WRKAIYKQLGWLAPMDLAEGVRRVTTWPYIDKSRVGIWGWSGGGASTLQSMFRFPDVF
                     HIGMCVSPVTDQRNYDNIYTERYMGFPKDNLEWFLKASPISYVQNLKGKLLLVHGTGD
                     DNVHYQNTEMLINALIAAGKPFDMMAYPNRTHDIDEGEGTTMHLYSLLTRYLKEHLKP
                     GPQ"
     gene            36815..38002
                     /locus_tag="PK754_00375"
     CDS             36815..38002
                     /locus_tag="PK754_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07988.1"
                     /translation="MKTFRRVIVWFAVICCVTVVSLLFEKPRDAALAFLFIIDMATPE
                     ASELYQVVTDDIVVTHDSLDRESKKTLYYSIYSPPGNHKKSAIIMAHGFTEKGNNDRR
                     LQRMAHRMARAGFIVMTPEFPMMRRFRISMEDADNTMEAFRIVKQRPDVDSSSIGLMG
                     FSYGAGPVIMALSDSSIRDQVRFGVIFGGYFDIRKTLKYTLTGYYTTDTGQEMRSVSK
                     NNDRWKFLLGNIPESGNEKSLVIFKSIIAQKITDAGYDPGKNFLSLDSTHQSLYRFIE
                     NQNPSAFDVLYAALPNDFRSKLELADLTSYAHLIHSYMIIGHSDQDRVVPYTESLALA
                     KNLTSAPAPFVAVTNLFTHVNLKIEWGSLTVIFTETIPGFFRLWKMTYHIMRMRHIPV
                     AIA"
     gene            38073..39206
                     /locus_tag="PK754_00380"
     CDS             38073..39206
                     /locus_tag="PK754_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNB07989.1"
                     /translation="MSCFFATTICGQNLYENLQVSSGDTPVDVSRRTKGRRFTPIPLV
                     NYTSDDGAGYGLRLCVYDYDSVTVPYCRALKAQYFITTEGKWAHVLSLDYPNFLKGHR
                     FDFELAYDKLEFANYYGELSNRMVDSLHLSKSQRTFRQATPSASLTWIYDLNGPWRLR
                     GGVSIRHTSVKPNADSGNILKELKPVGYKGGVLWKLDAAMRYDTRDNYINSTCGMLEE
                     LLVEYKIGGAGDYQGWLASYEHRHFIPITPNIVAGYRLAADQVFGQIPFYDELKLGGE
                     GSVRGMPSARRRGQGRFLVNTELRWHGIRLSDRRNMMLGVILFTDAGQIYKRKDGPSM
                     TPKNWHAGYGGGLRFYWHSTIIRADYGISEGGSGLYILFSQVF"
     gene            complement(39290..40525)
                     /gene="icd"
                     /locus_tag="PK754_00385"
     CDS             complement(39290..40525)
                     /gene="icd"
                     /locus_tag="PK754_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017250757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNB07990.1"
                     /translation="MYKDITPPKEGQKISIQNGVLQVPDNPVIPFIEGDGTGRDIWRA
                     SVRVFDAAVQKAYGGKKKIHWFEVYAGQKSFDQYNTWLPDDTITACKEYLVSIKGPLT
                     TPVGGGIRSLNVALRQTLDLYVCLRPVRYFNGVPSPVKRPEKVDMVIFRENTEDIYAG
                     IEWKEGSPEAKKVIAWLQNEMGVKKVRFPETSGIGIKPVSREGSERLIRSAIQYAIAQ
                     KRKSLTLVHKGNIMKYTEGAFRDWGYELAKREFGGVEIDGGPWVKLPNGVIIKDAIAD
                     ITLQQVLTRPEDFDVIATMNLNGDYLSDALAAQVGGIGIAPGGNINYITGHAVFEATH
                     GTAPKYADLDKVNPGSVILSGEMMFRYMGWNEVADLLIKGLETSIAKKTVTYDFARLM
                     DGAKEIKCSEFGDAIIANM"
     gene            40979..>42015
                     /locus_tag="PK754_00390"
     CDS             40979..>42015
                     /locus_tag="PK754_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNB07991.1"
                     /translation="MSVTSTGNSEQPTAKYDFTKIEAKWQAYWDQHKTFRVTEDPKFP
                     KEKRFYVLDMFPYPSGAGLHVGHPEGYTATDILARYKRMKGFNVLHPMGWDAFGLPAE
                     QYAVKTGVHPAITTKQNIDTFRRQLKMFGFSYDWDREVNTTDPHYVKWTQWIFLQLFK
                     KNLAYIAEVPVNWCPELGTVLANEEVPEQIDKGFTVVRKPMRQWMLRITAYADRLLED
                     LNLVDWPESIKEMQRNWIGRSEGAEIHFRIKDHDGDIKVFTTRPDTIFGATYMVLAPE
                     HPLVEKITSAEQRNAVEAYRKQAALKSDMERTELAKDKTGVWTGAYAINPANGNPIPV
                     WIADYVLINYGT"
BASE COUNT        12261 a         8320 c         9692 g        11742 t
ORIGIN      
        1 tgctcgacca taagccaatt atggtggtag gcaatattgg ccaaaagctt atggtattca
       61 ttgcgaaatt tcttttgatg aattcgttct tcgatacgca tgtctataat gtaaaaaaac
      121 aatgaatgag attaaataaa aaagaggcga cgaaccgaag ttgcgtcgcc tctaatataa
      181 ggggaaaagg ggtttacttc ttcttaacca tttcgaggtt aatggtaata tcaacatttt
      241 ctcctacgac aagtccgcct gtttccatag cggcattcca tttgaggcca taatcaaaac
      301 gattaacgga accattgatt ttccagccag atttggtgtt gccccacgga tctttgattt
      361 cgccgcgata cacagcgtcc aattccactg ttttagtggt accacgcatg gtcagttcgc
      421 ctgtaatttt gaatgtattt ttaccggttt ttttgaatga tttgcttgca aaagtaatat
      481 cagcaaattt gtcagcggca aaaaaatcgt ccgatttaag gtgcgcatcg cgtttttcga
      541 ttcccgtatt gatactgttg actttgattg tcatcgtggc ggtagcatca gaaaaatctt
      601 ctttgctgta acctagggtt gcatcaaatt ctttgaaata gccttgtaca tcggaaacaa
      661 caagatggga aacggtaaaa agaacgttgg agtgagaaac atcaacaccc caattggact
      721 gtgcaaatgt gcttatgttg ataagcaatg cggcggctaa aaccatcatg cgattcatca
      781 ggtatctcct atttttagtt tggactgtaa aattggttat gaattaattg gttaatatat
      841 gttataacat ttaatgttgc aacatttatt ttattaattt attcctactc aatagaaata
      901 aattaataaa acatcagaaa tcatgtttta tataagtaga agtagattga gttttttaat
      961 taaaaataag tattaaaggg aatgaattaa taaataaaac aatagtttaa aaaaatgtca
     1021 atcattgaaa tcactgttaa tatgataaaa gtcatacata gatatgacgt atatctttga
     1081 gaagaatttt ggaaaaaatt ataattaatc atcagaaatt ctgacagagg attcttttat
     1141 cctcattgaa attctaaaac cgtctaaaaa acaagcaagt ccccaaccaa tcaattatga
     1201 ctgaaacagc cgttcaatca cagcaaacgt gtattcattg cggggatata tgtgacaatg
     1261 aggtcgtgag actagacgga aaaacgtttt gttgccaagg ttgtcaatgg gtttatcaaa
     1321 tactgcatga acatgatcta ggtgaatact acgatcttca aaacggggtg cgttcgctcc
     1381 ctaaagccaa aaccaacgtt aaatatgaat ttttagatat acctgaggta cggaaatcat
     1441 ttatacagtt tgaacatgat caccgcgtcc gcgtaacatt atttttaccg caaattcatt
     1501 gcagtgcatg catttggctc ttggaacatt tgcataaaat ggaatccggt atacaatcgg
     1561 tacgtgtaga tttcctgcgt aaggaagcat atatcctatt tgatgatcgg ttaacctcat
     1621 tgcggcagat agcggaaagg cttgcttcga taggttatga acccgatttt tcaaggcacg
     1681 ataaatctgg ggctacagca ccggccgtcg agcgccatct atatatcaag ttaggtatag
     1741 ccggtttttg ttttggtaac attatgttgc tggcattacc cgactacctt gccggcgggc
     1801 agatcgaagc gccactgagg gattatttta gatatttgag catagtggta gcgctgccgt
     1861 tactatatgc agtgagcggt tttttttcgt caacatttaa tgcgctaaaa gaaaaaacga
     1921 tcacgatgga tgtaccgatt tcgatcggaa taacgacgtt gatcgttcgc agtctttggg
     1981 atatcaccca cggattcggc tccggttatt tggattcgct tgcagggctt actttttttc
     2041 ttttgatcgg aaaaatattt cagaaaaaaa cattttacac gttgtcgttt gaacgcaatc
     2101 tgaaatccta ttttccgctt gcggtacaga aaaaaacaga cgattcagcc gtagtgatac
     2161 cgatcgaaca agtgcaggta ggggatttat tgatagttcg tcatcaggaa cttatagttg
     2221 cggatagtgt attgatcaat gggattgcga cgatagatta tagttttgtt accggggaaa
     2281 gtatacctgt atcggtgatg cagggtgata aaatttttgc cggagggcgt caaaccggct
     2341 ccgctatcga agtggaagtg gtcaaagaag tatcacaaag ttatttgcaa cagttgtgga
     2401 atcaagaagc cttcacgggt tcatcggaaa gtcgaattca gaatatttcc aatacagtgg
     2461 ccaagtattt tatggttgcg gtattaacaa tcgctgttat tacaggactg tactggtacg
     2521 tgaccgatcc ggcaatggtg attgacacag tgacttcggt attgattgtt tcctgcgggt
     2581 gcggtttacc tctctccatt ccctttacat ttggcaccgc attgcgcgta ctgtcggagc
     2641 agcagtttta tattaaaaac tatcatgtcg tagaaaacct ggcagtgatc aatacgatcg
     2701 tttttgataa aacgggtaca ctgacgaagt cacaaggttc atcggtttcg tttgagggaa
     2761 gttctttgac aaaagagcag caagctatca ttgccgctgt agttcgtaat tctacacacc
     2821 cgcttagccg tgcggtatat gactatctgg atcacgttga aacgtatgtc gttgatcggt
     2881 atgaagaaat atcaggtaaa ggaattacag caaccgtaca tggtcatcat gtgaaaatcg
     2941 gttctttggc atggcttgaa ggattaacat tggcagaaaa ctctccattt tacagtgatg
     3001 cacaacgtag ccagatgcat atcgtgatag acggatcact tttagggtgt tttacaattt
     3061 tatcagaatt tcgtgaaggc acagaatacc tgagcaaagt cataccgaag gactatcaac
     3121 ttttcgtact ctccggagat agtgaccgtg aaaaaaatcg gcttgagcaa ttgtttacta
     3181 accgtgcggt aatgcttttt aggcaattac cgatagataa actacaattt attcggcaac
     3241 aacaggtatc cgggcgtcgg gtattgatga taggcgacgg actcaatgat gctggggcgc
     3301 tcaaacagag tgatgtaggc gtagctattg cagaaaacac ggccgctttt acgccgagtt
     3361 cggatgcgat catggatgtt aaagcgttgc ctcagttggc gcgtttttta attttttcta
     3421 agcaaactaa aaaaacagtg tacgcagcgt tcggactcac cctgttatat aacttggtgg
     3481 ctgttacttt ggcggtgcaa ggtcttttgg cgccggtagc tgcagcggtg atcatgccga
     3541 tttcagcaat ctccgttgta gccttcacgg ttatagccgt tcgatggata gcaagaaaaa
     3601 atcaactttt aaaaacggta tcggacgtac cgataccttc attgagcaac gcatgaatat
     3661 attagttgta cttatcgccg tcagtctgat gctggcgttt ggttttttag cagcattttt
     3721 atgggccatg aaccgagggc agtatgaaga tacacacaca cccgcgatgc gtatgctatt
     3781 agatgaaaat caaaatcaca taacctcaaa cccttcatca tccacggaga attaaacgtc
     3841 atggaaaaga catccgtagc gcatgaaccg caggtgacag gtcggctcga acaatttgag
     3901 tacgacaatg acatcgtgcg gaagtttgct attgcatccg ttgttttcgg tattgtcggt
     3961 atgctggtcg gcgtgatcat cgccatccaa atctatatgc cggcgctcaa cctagatatc
     4021 ccttggctaa ctttcgggcg catccgaccg ctacacacca atgcggttat atttgcgttt
     4081 gtcggcaacg ggatttttat gggtctctac tattcgctac agaggctttg taaagcacgg
     4141 cttttcagcg acacgttgag taatattcat ttttggggtt ggcaattgat cattgtttcg
     4201 gcagcgatca ccttaccgct cggtattacc agcggaaaag aatatgctga attagaatgg
     4261 ccgatagata ttgccattgc gctcgtgtgg atcgtatttg gtatcaatat gttcggtacg
     4321 atcatcaaac gccgtgaacg acacatgtac gtcgcgattt ggttttatat cgccacatgg
     4381 gtaacagtca ctatgttgca cgtggtcaat tcgttggcca ttcctgcagg atggtttaaa
     4441 agttattcgg tgtttgccgg tgtgcaagat gcgctggtac agtggtggta tggtcataat
     4501 gccgtagcgt ttttccttac gacgccgtat ctcggattga tgtattattt tatgcccaag
     4561 gcggttaatc gtcctgtgta ttcttatcgt ctttcgatca ttcacttttg ggcattgatc
     4621 tttttataca tctgggcagg gccgcaccat ttgctttata gtgcgcttcc ggattgggct
     4681 caatcgttgg gttctgtttt ttcgatcatg ctcattgcac cgagttgggg cggtatgatc
     4741 aatggtcttc ttacgttgcg gggcgtttgg gataaagtgc gtgaagaacc ggtgatcaaa
     4801 tttatggtgg ttgccgtgac cgcgtatggt atggcgactt ttgaaggtcc gatgctttca
     4861 ataaaaaatg tcaatgcgct tagccacttt acggattgga cgatcgcaca cgtacatatc
     4921 ggcgcactcg gatggaacgg gttcctgact ttcggtattt tatactggct tgtacctaag
     4981 atgtggaata ccacattata ttctaaaaaa ttagccaacg cgcatttctg gctgggattt
     5041 ctcggtatag tgttttacgc ggtgccaatg tattgggccg gtatcacaca aggtctgatg
     5101 tggaaacaat tcacaccgga cggcacctta caatatttta attttcttga aacggtaact
     5161 cagatcatcc cgatgtatgt gattcgttcc atcggcggta cgatgttttt tgcatccatg
     5221 tttatcatgg cttataacct gatcaaaacg gcgaaacaag gccagtttat tgccaatgaa
     5281 aaagcagaag cgccggcatt aacaaaagaa tatgtgtcac acggtcaatc gtattaccac
     5341 cgtttgatcg aacgcaaacc tttaccgttt atcattttga cactgatcgc cgttgctatc
     5401 ggtggagttt tagaaatcgt acctacgttt gcagtagagt ctaatatccc taccattgcc
     5461 agcgtcaagc cttattcacc cctagaattg cagggacgtg acatttatat ccgcgaagga
     5521 tgttatactt gccactcgca gcttgtgcgt ccgttccgtt ctgaaacgga acgctatggc
     5581 gaatactcca aagctggaga atatgtttat gatcacccgt tccaatgggg ttctaaacgt
     5641 acaggcccgg atttgcatcg cgtcggtggt aaatacacga attcgtggca ttatagtcac
     5701 atgatggatc ccagagccat ttcaccgggt tcgatcatgc cggcctatcc gtggcttttt
     5761 gaaaacagcc tcgacatatc aacgacggaa aagaaaattt cagctatgca gatcgttggt
     5821 gtgccgtatg aaaaaggata tgaaaaagta gcaaataatg atttgaaagc acaagcaaca
     5881 gccgtcgcag gcgatctgac acaaaacgga tttcctacga caccggataa agaaatcgtt
     5941 gcattgattg cctatttgca acgactggga accgacatca aggtgaaaca ataaatgttg
     6001 cgtcgaattt tagaaaatgc aggtggtctg gaaatatggc cgaccatagc gctcgtgatc
     6061 ttctttgttt tttttgtggg actgatcgca tggcttgtgc gcatagataa gcggcatctg
     6121 catcatatgg aaacgatgcc gctcgatggc gaagaaatcg ctgtatcaaa caacggatac
     6181 aaacacggag gaaaataata tggctgaagt caaagatgag ttgctcgatc atgattatga
     6241 cggcattcgc gaatatgata atccactccc gccctggtgg gtcaatatgt tttacatcac
     6301 gatcgtttgg gcgaccattt acttgatatt ttatcatgtt ctggaaatcg gcgaccgctc
     6361 ggtggatgaa tacagaaaag aatttgacaa tacatggacc aaagaaatgg atccaaatta
     6421 tcaaaaagtt gatatgcttg gtaccggtta taaaggcccg tattactata agccaagcga
     6481 tccgaccaat aaaggtgttg tagcctttat gccggccgct gaaaaaccaa aagaagaaga
     6541 actcaccaaa gaatttgccg cactgacgga tgcagtaagc ctcggtaaag gcaaagagat
     6601 ttttaccgta aactgtgtgc cctgtcatgg taaactgggt gaaggcggta tcggacccaa
     6661 tctgacagac gattattgga ttcatggtgc gggtatcaat aatgtcgtgc gtaccgcatg
     6721 gaaaggtgtt cctgaaaaag gtatgatctc ttggaaacaa cttggcgaaa aaaatgtgtt
     6781 attggtggcc agttatattc ttacactacg tggtaccaat ccaccgaatg ccaaagcgcc
     6841 tcaaggcgat ctggtaaaac aataaaaaga aagttaatta cgcggcagta tggctgaggt
     6901 aaaagaggaa ttagcgcaga tcaagaacgc ggacggtgaa acgtatcgtg accgtatcgg
     6961 gattattgat aaaaaaggcg cacgtatttg gatttaccca aaacttgttg ccggagtatt
     7021 tcactactgg cgagtgattg tttcgatcat tctattgttc gtgctttttg gtgtgccgtt
     7081 tatcaaagtt gatgagcacc cttttatcat gttcaatata cttgagcgaa aatttattct
     7141 ctttggtttg gccttttggc ctcaggattt ttaccttttt gcgcttacgt ttctttctat
     7201 tgtcatattt atagtgctct tcacctccgt gtttgggcgt atctggtgcg gctgggcgtg
     7261 tcctcaaacc atttttatgg aaatggtttt tcgaaaaatc gagttttgga ttgaaggtga
     7321 cggaccaaaa caatacgcat tggatcaagc accatggaat gcagataaaa tcctcaaaaa
     7381 aacatttaag catgttatct ttttcgcgat ctcatggatt atcggtaaca ccttgctcgc
     7441 gtatattatc ggtgtagatg aattattcaa aatcatcacg gatccgatcg gtatgcatgt
     7501 caacggtttt acggcgatga tgattttcag tttgatattc tatggtatct tttcccgttt
     7561 tcgcgagcag gcgtgcatct atgtatgtcc ttatggtcgt ttacagtcgg tattgctaga
     7621 taaaaactcc atcgttgtca tgtatgattt tcagcgcggg gaacctcgag gaaaattaaa
     7681 aaaagggaat accggcggcg aaagtttggg cgattgtgta gattgtaaat tatgtgtaca
     7741 ggtatgtcca acaggaatag atattcgtaa cggtacgcag atggaatgcg ttaactgtac
     7801 ggcatgtatg gatgcttgcg actctgtcat ggaaaaaatc aagcgtccta aaaacctcat
     7861 ccgctacggt tcgtataatc ttatttcgca aaaattgcat ttcaaaatta caccgcgtat
     7921 tgtcttatat tcggctgttc tgactgtatt gattacagtg acgtccattt tccttttcaa
     7981 acgtaaagac attcaaacta cgattttacg tgcgaaaggg cctatcttcc aacttatgga
     8041 gaatggtgat atacggaatg tgtatacagc caaagtactc aataagacgt tcgatgcaat
     8101 gcagattgag tttaagctcg aaggtgtgga agggaatatt gcgctgatcg gtacccatga
     8161 attgcggttg catccggacg aattggtaga gtccacgttt ttagtagata taccgcgtaa
     8221 ttctattcat acaatgaata caaaaattaa aataggtgtg tataaagacg gaatgaaagt
     8281 ttcaaccgaa gaaacttcgt ttgtgtcgcc catgcagtga aaggaagagg catgtcgtta
     8341 ttcagaaact ggggattccg aatcactgtg ctctatgtca gttttgcgct tctcattctt
     8401 ggaatggtat tcgtttcctc catcagtcgt gaggatttgg taagtaccaa ctattacgac
     8461 aatgagattg cttatgaaaa gcgaattcaa gcgatcaaac gtacgaagat gttggaacaa
     8521 aaaccggcat ggaatatttc tgggcgtgtg ttaacgatga tttttcctga gcaaatctca
     8581 caaatcacag gaggtacact gaccttgtat cgtgcatcgg atgctgatcg ggatatcgtc
     8641 gtacctctga agcttgacag ccaacgatca caagcgatag atttacaaaa agcaataccc
     8701 gggatgtggc gtgcaaagtt agaatggaaa tcggaaaata atgactatta tcttgaagat
     8761 gtaatttaca tcgaataaat cggcgagtca cttattatgt ttgaaatata ctcagcatta
     8821 ttggtgggtg tacttggaag tttccactgt atcggcatgt gtggtccatt ggcattagcg
     8881 ataccgcagc atgatcgccg gtggtttatg ctggtaagat cagcattact gtacaatggc
     8941 ggccgagctt ttacctatgc catgatgggc ttggttgtag gggcaattgg tgcgacgctg
     9001 cagaccgttg ggcttcaaca aggattatcg atcgggcttg gtgtggccat tttattagcg
     9061 ctccttttgc cgaattcgat caaacaaaaa atatggccgg actcatcgct ccagggcggt
     9121 tgggcagcga tgcggagatg gtttggtcag cttctgcgtg atcccaatcc cacatcactc
     9181 ttcacactgg gtttactcaa cgggttttta ccgtgtggat tagtctacat agcgctcggc
     9241 ggtgctatgg ccacaggtca tgcgtgggaa ggtgcgttgt atatggcatt gtttggattg
     9301 ggcacgttgc cggcaatgac cgctgttgca atttttggtc aattcattac caccacaacg
     9361 cgccagaaaa tcactaaact gattcctgtc gtagtaggtg gcgtggctgt tttattgatt
     9421 ctccgggggc tgtctcttgg catcccgtat atcagtccgg atatgcacgc ggtggctgcc
     9481 ggcagcggtg aatcctgttg tgccaaacat taatctcaat atgtcctttt atacacgttg
     9541 gcaatcttat cgctggcctg tagtagagaa tcttttaggc acacgaacaa aaaaatcggt
     9601 agaaaatatc cttgccgatg cgtctgattt taaatttccc gaagacgtta ttactctgct
     9661 ttctccggct tctgacacat atcgtcatgt aatagcacaa caagccgcgc agcaaatcca
     9721 acgcccgatt catttgtaca cccctcttta tctgtcaaat tattgtcaca acagttgtac
     9781 gtattgtgga tttcgcgtag aaaataaaat tcaacgcgtt gtgctcgatc aaacacagat
     9841 cgaaaaagaa ttacaagcga tagcggcgac gggtgttcgt cgtgtgttat tggttacagg
     9901 cgaagagcgc cgttttgcag gcgtaactta tattgcggag gctgtgcgta tagccaaaac
     9961 attgtttgat catgtacgga ttgaggtaca accgttgaat acggatgact atgctttgct
    10021 ccgcgaagcc gggtgcgaag gggtttatat atatcaagaa acgtatcatg aaactaacta
    10081 ccgtctatat catccgcgcg gaatgaagtc gcatttccgc catcggcttg aaacaccgga
    10141 tcgcatcgcg atgtcgggga tttcgaaaat cggattaggt gttttgctcg ggcttgagaa
    10201 ctggcgtgtg gatgcgtttt ttttagccat gcatgcgatg tatctcaaag aagcgtatcc
    10261 caagttgcaa atcgctattt catttccgcg tattcgacct gctgtcggtg tgcataataa
    10321 agtctccgat gtatttgaga gtgatttgca tcatttggtt gcggtatttc gatggatcaa
    10381 tccggattgg gatatagcga tgtcgacgcg cgaatcacca gattttcgca atcgcacatt
    10441 gaagcttggc gtaacctcaa tcagtgtcgg ttctaaaaca caaccgggtg gttacttgaa
    10501 aaacgataca gccgagctgg aacagtttgc cgtgcatgat aaccgttccg ctgaaacaat
    10561 gcgcagtttt ttagaaaaga atgttcggat gtatgattga acaaacacag cattaaaaaa
    10621 ttagctgata gagtgttgtt taaaaggagt ttagaataaa ggatacgaat tgcaaaaacg
    10681 gactttattg catgaataat aatcaattac ttttttatta aaaacttctt gcattgtctt
    10741 aggtcgttta ttatattgcg tcccctaaaa tgcgtaagtt ataatatttt tatacattta
    10801 ggctctaagg agtttatacc gtgaaagttt cgaacaagac attgatgccc aaggccgacc
    10861 aaatcaaaca gaagtggttc gtcgtggatg ccgctgacca aacacttggt cgctttgcca
    10921 gccgtgtagc gtcaattctc aaaggcaaaa acaaagcgta ttatgcgccg catatggatt
    10981 gcggagattt tgtggtcgta gtgaatgcgg acaaagttcg cttgaccggc aaaaaaacaa
    11041 aaatcaaaga atacattcat aatacgatgt atcccggcgg acaacgaatc actaagtttg
    11101 aagatatgat tcgtaccaaa ccggaacgcg ttgtgtattt ggccgtgcat ggcatgctgc
    11161 ccaaaaacac gctcggcgct aaaatgataa agaagctgaa aatttatgcg ggcgaaaaac
    11221 atccgcattc agctcagaat cctgaaacgt taaaatttta atttataaac aggagtgacc
    11281 atgagcgcac cattagcagc tgttggccgc cgcaagaccg cgaccgcacg agttcgcctc
    11341 gtacccggca acggcaaggt gacgatcaac catcgcgaca tcaaagatta ttttccacgc
    11401 ggatcgcatc agattcacgt gaccgaacct ttgacggtag tcgaagccgg ttccaaatat
    11461 gatgtgattg ctttgacccg tggcggcgga ccgagtggtc aagccggcgc gattcgtctt
    11521 ggtatcgcac gtgccctcgt aaaaatcgat gaagagatga aaaaagctct gaaacccatc
    11581 ggtgcaatga gccgcgatcc ccgtatgaaa gaacgtcgta aatacggttt ggctaaagct
    11641 cgtaagcgtt accagttcag caaacgttaa ttcgttattc gaaaaattgg tgtcggtcat
    11701 acgcgctatg tgtgtgaccg acttctttta tatttttaaa caaagcactg tatgaatcat
    11761 tcagtgcgta ttttcaaaca atcacacttt ctgatttgaa cgagggtgcc ccgctaccgg
    11821 cggagtttcg ctcaaaagcg acgaaagtgg tcgtttaacc ctgtaaggag tttctcatgt
    11881 ctgaagtcca agtctcattt gatcccaaaa agaaaacgga agaactttta gcggccggcg
    11941 tacactttgg tcacttgacc cgccgttgga atcccaaaat gaaaccgtat attttcatgg
    12001 aaaagaacgg tattcacatt ttggatttgc gtaaaacggt ccattcactg gaaggtgctt
    12061 gcaaagcgct ggcaaaaatt gttgccaacg gtgaacgtgt tctttttgtc ggaaccaaaa
    12121 aacaagctaa agaaatagtt caaaatgaag ccaaacgcgc caattccttc tttgtgacgg
    12181 aacgctggtt gggcggtatg ctcaccaatt tctcgacaat tcgtaaaagc atcaaacaca
    12241 tgaagaatat cgagaaaaaa gaaattgacg gtacgtttga aaagattact aaaaaagaac
    12301 gcctcatgtt ggagcgcgaa aaagccaaac ttaagatcgt actcggcggt atcgaagata
    12361 tgaaacaatt accgggtgcg gtatttgtcg tggatgttaa aaaagaacac atcgccgtca
    12421 aagaagccaa tgtgctcgga attcccgtat ttgctatcgt ggataccaat tgcgatccgg
    12481 atacagttca gcacgtaatt cctggtaacg atgatgcgac caaatccatc gaagcgatca
    12541 ccaaaactat agccgatgcc gttgcggaag ccaaccaaca cgccgtagct gccgctaaag
    12601 aaaaagaaat cgccgaagtg gatgaagaaa gagtcaaaga tgaaacccgt atgaagtatg
    12661 aagagacgaa ttttaacaaa aagcgtcttg gtaaaaaaga agtgaaaaaa gaagattaat
    12721 tccgtgtacc ggaattttac aaacctcgga acaggagttt tataaaaata tgtcagctgt
    12781 agaaattagt gcctcggccg taatgaaatt acgcgaagaa accggcgcgg gtatgatgga
    12841 gtgcaaaaaa gcactcactg aagccggcgg ggatgccgcc aaagcggtag aaattctccg
    12901 tgaacgcggt ctcaaaaaat cagccgaaaa acaaagccgt gttgccaacg aaggtaccgt
    12961 actttcctcg gtcaacgatg cgcacaattt tgcggtcatg gtagagatta actgcgaaac
    13021 ggatttcgtc gctaaaaatg atgatttcgt aaaattttcc aataacgtat tggcgcaagt
    13081 gcgtgattcg aagccggcca atctggatgc atttttatct gcccctgcgg cctttgctgc
    13141 gggtcagacc atagcggatg ccgtgaccgg attgactgct aaaatcggtg aaaaaatcgc
    13201 cgtcaaacgc attgccgtga tcgagaacaa tcaaggcgtt attgcgggtt atattcaccc
    13261 cggtaataag ttgggcgtgc ttctgcagct caatgtcgaa ggttttgccg caacgatgac
    13321 tgcggatgcg tattccgtcg cttatgacat tgctatgcag acggctgcga tgaacccgac
    13381 gtatatccat cgcgaagaag ttccggcgga tgtggttgaa aaagaatcgg ccattctccg
    13441 cgaacagctc aaaaacgatc cgaaaaatgc aaataaaccg gacaatgttt tgactaacat
    13501 catcaaaggt cgccttgata aattttatca ggaagtctgc cttgttgacc aggcattcgt
    13561 aaaagatccc aataaaatga tcaaagacct tgttgccgat ctctccaaaa aattaggcaa
    13621 accggtaacg atccggagat ttgaacgctt tcgcctcggt gaataatctt ttaaaagcca
    13681 cggattttaa ttcgtggctt tttttatgtc ctttttataa ttttacctgc tttcaataga
    13741 agtcctgaac tgcaggcgta tcgcaaatac aaggaatcat accatgccca tgcccatatt
    13801 caaacgaatc ctcctcaagc tcagcggcga agcgcttatg ggtgaacagg gatacggaat
    13861 cgataccaaa gtgctcgaac agatagccaa agaaatcaag gatgtcaaag ccttgggcgt
    13921 cgaaatcgcc attgtaatcg gcggtggcaa tatttttcgc ggcttagcgg ccagtgccaa
    13981 aggtatggat cgcgtatcgg cggatcacat gggtatgctg gcaacagtga tgaattcgct
    14041 ggcattgcag gatgcgttgg aaaaacaagg actctttacg cggatactaa gtgcgattaa
    14101 aatggaacag gtttgcgagc cgtttattcg ccgtcgcgcg atacgccatc ttgaaaaagg
    14161 tcgtattgta atctttgcgg caggcaccgg caatccgtat tttacgacgg atacagcggc
    14221 gtcattgcgt gcgatcgaag tggaggccga tgtcatactc aaaggcacgc gggtggacgg
    14281 cgtttacgat tccgatccgg agaaaaacaa agaagcgttc cggtttgaaa agatcactta
    14341 tctggatgtg gtcaaaaaag gactcaacgt catggatatg acagcggtga ccttgtgtat
    14401 ggataataaa cttcctatag tggttttcaa tctaaaaact caaggcaatc tccgccgact
    14461 tgtactcggc gaaaatgtag gaacacgcgt tcagcaagaa caataaaaat ctaaagaacc
    14521 gaattaaata attgaggtgc tgttatgtac gacctgaata agcaatatca cgaaaccgaa
    14581 gaaaaaatga aaaagagcct cgagacggtg cgcaatgaac ttgctaaaat tcgcagcggc
    14641 cgtgcgacga cgacacttct ggatggtgtc aaagtggatt attacggcac accgacgcca
    14701 ctcaatcagg ttggtaatgt ttccgcgcca gaagcgcgat tgcttacggt acaaccatgg
    14761 gaaaaaggta tggttccggc tatcgaaaaa gcgattcgtg aagcgaatct gggtctcaat
    14821 ccgcaaaatg atggcgcgtt gattcgcata cccattccgc cgctcaacga agaacgtcgt
    14881 aaagaattag tcaagctcgg taaaaaatat gccgaagaag gaaaagtggc cgttcgcaat
    14941 gtacgtcgcg atgccaatga gcacttcaaa aaagccgaaa aggatcataa gatttcagaa
    15001 gatgactcca aagacgctca ggacaaagtg caaaaaatga ccgacaacta tatcaaaaaa
    15061 atagacgaaa tgttggtaat caaagaaaaa gaaattatgg aagtgtaatt tgattcgcga
    15121 tataggtttt tttaagccca cacctaatag atgtgggctt aaaaatttat tgtgtagcgt
    15181 tgtacgtttt tagaagggtg tcataaaact gtatctgcgt ccacttgtga tggtgctaat
    15241 acccatcttc tgacgaattc caagtattca gggataggtt ggggtcttcc cttgatgaat
    15301 ctaaacaaac taaatttcaa tgattctacg gaatcttcaa ccgattgaat gaggtgtccg
    15361 gcatttctgt acgttttgat tctgtaatta acttgatcgg cttcgataga atcttccaat
    15421 cgctttattg attgcacaac cggtatgtgt gagtctttac taccataaac aaataaaaca
    15481 ggtgttttta cttgattcca aaacggcatg ggatcaaaag tactattgcg ataatgatcg
    15541 tactgtggtt gcttcaaagt gtcctcgggt gacagatcag gattataatt gaaatctctg
    15601 aaccattgcc gatccttgaa acgatgcttc atctgcaaga ctttttcccg ccctacaaat
    15661 gttgcaacat attctgtaac agcacgatta tagtttaaga tatcgtcaat atcgttctgt
    15721 gagtaaccag cctcgattag ttcacgccct ttttgaaaga gatccgattc ataaggaggt
    15781 actgacgggc atgaagtaca aacaacaaac gacaaaccat caagattttg agctgtaatc
    15841 ggaagaaccc aggccgcttg actgatcccc acaatcccaa ttttattctt gtcaatatca
    15901 ttccgactgg ataatgactg aaatgctgct ttcagatcgg aagcaagaag ttgaaaatca
    15961 ggactatcgt cgtaaactcc ttccgaatcg cctaccccgc gtttatcaaa tataaataca
    16021 gcaatcccgt gttttgagaa atactcacca taaggtttat aaaaaaagga gtttctatca
    16081 gccgtgccag atccatgtgc gatcactatt accggataag gagctgaggt caacggcttg
    16141 taaaaggttc cggaaaaccg aatctggttg ttgtaaaaaa caatagtttc agtgtgatat
    16201 agcgtacggc atgaacataa aaccatagtc actatggcaa gtagaaaaaa ccagctaact
    16261 cttttcgcca tattagcctc aaagaagaga ttgtgagttt gaaatcggaa aagtaatgtc
    16321 aaaacgtcaa aagagaaaaa aagtttcaca gattgttgaa tataacgatc acaaaaattc
    16381 aaatttcatg ttattcaact tgaaatgatg cgatgaacgt aaatcgcgtt ttgaatcgaa
    16441 gtcagggata caatcacatg gttatgtttc aattcacgaa aaaaaagttc tagtattcaa
    16501 tgattttcca aaaaagagtg tcttcaaata aagttgcgat aacatttgag tttcttaatg
    16561 gaaaaattat atgtgaaagc ttagcattct atatacacca aaaaactaat gttaaacgaa
    16621 ttccggaaat actgtttaat gatgtatgct tgagaatcca aacccatgac tgtaaattta
    16681 cattttggct atcgcaatta aagctaagtt ttttgtatat tatatatgta cggaatatgt
    16741 ctttgcaata gaatgccata cagtaaatat gttagttaat gcgaaaatac gtactatcat
    16801 acaatgggaa tttactcagc gtgtgatttc aaagggtttt cttttttcag tcatttttat
    16861 tcctgccatt attcttgcct ttacattgct accggtgttt tttgccaatt ctaacggcaa
    16921 tcgtattttt tctgtcggcg tatttgatgc cacccggcgt ttgggcgaag atctatcatc
    16981 gaagctgcaa gatttgacgt atagcgaaat tcagctcgta cctttggctt cgtccaatgt
    17041 ggaagaggca ttagcagagg gtaaacgcct tgccaaggaa gacggaattg atgcggtgct
    17101 ggttttaact gcggatatgt tcactcagcg aaagatagaa atatataccg actaccgtac
    17161 cgagaatgcc gtacagatag aattgttaaa acaagcggtt gccggtctgc tgatggaata
    17221 tcgtatgcgt ggtaaaaatt ttacacacga agaaattcaa aacatcaccg ctccgttaca
    17281 agtagacaca tataagctag gcagtgatca tgccgcttcg ttacaaaaat acatgaccgg
    17341 gattattttg gtgatgatgt tgtattttgc aattgccaat tcggccggat catttatgcg
    17401 ggggctatcg gaggaaaaaa acaaccgtgt cattgaagtg cttgtcgcca gcgcctcgcc
    17461 gagagaaatt atggcgggta aagtcatcgg tctcgggcta gtcgggttgt tgcaaattgc
    17521 agtatggaca acattggccg ttatgttggg atcacgtaaa gaaatcgccg cactttctcc
    17581 ggagctgcta ctttttttta ttctctattt tgtgatgggc tatatgtttt ttgcaggaat
    17641 caatgctttg atcggagcta ttttcgcttc agatcaggat atacagcctt tacaagcggt
    17701 catttcgatg ttgggtgtgg taccggtagc attggcgttt tgggttattg aagtgccatc
    17761 cgatatcgcg atacgaatac tttcatacgt tccatttcta acgccgacga tgatgattct
    17821 tcgtattatt gtatccaatc cgccgtggtg ggaaataggg ttgacattca taacactttt
    17881 tgtcggtgta gtcttggtgt ttcgtatggc cggtcgttta tttgaaataa gtttgcggat
    17941 gcatgggaaa aaaatatcat ttcgcgagat ggtgcgatgg gctcgggcat aaaaattaaa
    18001 gttactactt tagccataaa aaatatggtt tgcgaccgat gcatccgcgt ggttcgtgaa
    18061 gaattggtta atatcggcgt aaatgtgcgt catgtcgaat tgggtaaagc gatcgttgag
    18121 ggtaatctaa ctttgccgat gcaaaacaaa ctacgaacgg tattgactca aaatggtttt
    18181 gaacttcttg aggacaaacg tgcaaaaatc gtagaggaaa ttaaaaatca aatcgtgatc
    18241 tatgttcaaa aaaacgatgt cgaaaataaa aatattaaat tatccgtact cttatcgcgt
    18301 acgatcggtc gtgattattc atatctttcc ggccttttct catctgtaga agggattacc
    18361 atagaacgct ttttgatttt gcaaaagatt gaaaaagtga aagaatggct agcgtacgac
    18421 gatcggacgc tcagtgagat ttcatacaaa ctcggataca gcagcgtgcc tcatttatcc
    18481 aaacagttta aacaaataac gggcttaacg ccgacggaat ttaaacagca aaacgatcat
    18541 cgccgaattc cgttggacaa aataaaaata atccatcgta tcacataacg ggaaccaacg
    18601 atgcgccaaa aatattatct tgtatccgga gccggaacgg gtatcggtct tgcaatagcc
    18661 aaaaaattag ccgaacgtgc cgatcacagt gtaatacttt gtggtcgccg gcaggatgtt
    18721 ttaagcgaag ccatgcatca actctccaat acgcgcaatc attcgatatt ggtgatggat
    18781 atacataatc cgggcacgat aaaaaaagcg gcggcccaac tggctatacc gtatctagat
    18841 ggtattattt ccaatgccgg tatcggagga gaaaatcaat atggcgacga agatcgctgg
    18901 gatgcgatca ttgcaaccaa tctgacaggt acatatcatt ttgttaataa ttttctacca
    18961 atcgtaagaa aatcgccgaa tacataccgt catattgttg ttgtttcgtc tattttagca
    19021 agacttggcg tggcaaggta ttcggcctat tgcgcgagta aagcaggttt gctcggtctg
    19081 atgcggagtt gggcggttga gcttgcacct gagaaaattt tggtcaacgc ggtctgcccg
    19141 ggctgggtca atacaaaaat ggcacaggat ggtatggaag gtatagccaa aaatatcggt
    19201 gtaacgacgg aagaatttta tcggatggct atggaatcgg tgcctttgcg gaaaatgagc
    19261 gaaccggaag agatagctga tctaactcat tatctgctaa agcaacgcag tataaccggt
    19321 cagacgttgg atatcaataa cggcgcgatc atgaacagct aagcttctat ttttgttgtt
    19381 attttaaata atgtgagtta gattaaactg tctaagataa gtgagtacgc tcatgaaata
    19441 caaagtaacc accgaacaaa aagagtacga catcgaagtc gtaccgcaag gcgcaagctt
    19501 caaagtgcgc gtaggcaaat ctcctgatat agaagaattg gatgtagatt ttcacaccgt
    19561 aggccaggga tcggttcatt cgatgctggt caatgacaaa tcgtatcgtg tgattattga
    19621 taaaaaaatg cacaaacacg ccgtatatac ccggcgtcat cgttttgagt ttaatgtcga
    19681 agatgaacgc acatttttga tgcgttcatt aatagccggc tccggagcta aagcggtcgg
    19741 tgaagtaaaa gcgccaatcc ccggattggt cagcaagtta cttttacaag aaggcgacac
    19801 ggtcaatcaa gggcaaggtg ttctgatact tgaagcgatg aaaatggaaa atgaaatcaa
    19861 agcgccgatg gccgggaaga ttaaaaaatt tcatgtgcaa ggcggaaaaa atgtagaaaa
    19921 agggcaacct ttgtttgtag tagaaccata acgtacgcgc ttcaagggtc ttgatatatg
    19981 aataaaaaat tcgaacttcc gctatgcgat caatgccatt cacgggttca ttccgttttt
    20041 ggtgatctca aagacagcga tgtcaagtcg ctgaacgaaa ataaatgcgg caatatttat
    20101 aaaaaaggcc aagtgctttt ttatgaaggc aataagccga caggtttgtt ttgcgtcaat
    20161 aagggtaaga taaaaacgta ccgcatcaat gcggacggta aagaacaaat cattcgatta
    20221 gccaaagaag gcgatgtttt gggatacagc tcgctcatta gcggcgattt gtattcgagt
    20281 tcggcggctg ttatcgaaga cgcgattatt tgttatatac cgcgtaatgt gtttttgaat
    20341 ctcctggatt ccaatacgac attttcgatg aagatgattc gtctgctctc gcatgatctc
    20401 aagtcatccg aagatcgcgt cgtcaacttg gcgcagaata ccgtacgcga acgcctcgcc
    20461 gaaacattac ttatgcttga agaattcggg ggaacggaaa atgacggtaa aacactacgg
    20521 acatcgttat cgcgtgaaga aatagccaac atcgtcggaa cggccacgga gacaaccatt
    20581 cggctgctat cggaatttcg cagtgatggt ttgattgaat tggaaggaaa acgcatcaag
    20641 attgtggatc ggggattgct actcaaagcc gcaaacgttg aagattaaaa ggtttaaaat
    20701 agaaaaaatt ttagaaccaa tagattataa cagacagcca gcgctacgaa cgttggctgt
    20761 tcgcttttga ttgcggtgtg tttgtcaaaa cggaattggg ggcgatgaat gtcttcatgt
    20821 accgtacggt attttggaat aaaccgcggt gtcaattggg agtataattc ataatccgag
    20881 ctgaattttt catgcagata tgtttcttca taaataacga tacagtaata gatcattcca
    20941 aaaaaaataa atacgcaagg tattgcgagt atcaagaaag agtttgacaa ggcggtaaaa
    21001 cctaaatata ttaaaatatt acccgtatag atcggatttc tcacatacgc gtatggtccg
    21061 gattttatta aaataggggc cgtaacgccg gaaacgcggg attcctgtcc gatatatccc
    21121 acagtcataa agcgtaacga ttcaccgata acgatcagta acgtacctgt aataaatggt
    21181 atggcgtcat aggtgcccgt aataagaaga aaaatgtata atgggacggg agtaaaactg
    21241 cgaaatctaa aaaagacttt cccaagtctt gaaattacca tcggttaagt ttatatttta
    21301 tctaggttac gtgtgacaca tatcacagca ttataattaa ttcaaactta atttactcaa
    21361 aaaatgccaa aagaatatct ttttcatcac aatattataa ggagaaacgg tatggaactt
    21421 aaggctgtat taagtcggtt ccgccaaatg gtcgggttgt cactgatcgt tgcggtattt
    21481 tcgttcacta cggttgttgc gcaaggaaca acgaatgggg taacaattac tcctgaacca
    21541 ttgacaccgt tcacctttac ttcgtttcct ttaagccttt tgggtattgg cggttccgat
    21601 gccaacttat cttcatttaa taatttcggg ttgagatttg agaataatag cggatctgtc
    21661 aaaataataa gaatgggtat agacttaaaa acgtctcagg ggtcgatatt taataagaaa
    21721 tatttggtga ctctaactat tccctccggc gtgccgattt ttgtaggcgt tccgcagata
    21781 ttgaacggca gttttggttc tatcaaagca tcaaacaggc aatcaattag tattagcgat
    21841 gactttatga agcagatcaa aggaggcttt cccagcggtc ttttcacatt tgatattaaa
    21901 gcgggtcttc ccaatgttaa taattatgat ggtttggatt atatggggtc gcttcagatt
    21961 caagttctcg gaaatagtcc cgttgagatc atttctccgt cttttggctt tgaatctata
    22021 gacggacttc ctcaatttgc atggtctgcg caaggtgcga cgaaattttt attgactatt
    22081 gctcgtaaaa aaggttcgca atcggatgaa ggtgccatca aatccgtggt acaacgtgca
    22141 gtgatcgaat tggataatgt gttttcatat agcctgcctt caggcggtcc ctcgacctcc
    22201 aaagaaaaca atcttaattg taatccgggt ttagagccgg gtgattatgt attccaagtg
    22261 acagctgtta ttcaagataa tttcaccggt aaatcccgca atgtagacag tccgattcgg
    22321 gcctttacag taaaagaaac tatatcatca acagccggcc tcaatacgga agaaatcctt
    22381 ggccttttga atgatgcggc taaaggagcg gttaatttca aagatcaact taaaggtttt
    22441 aaagcggttt ccattagtat caaaggtaat acagctacca tcgatgatct ccgcacagcc
    22501 gtacaaaacc tgcctgaagc tgtaaaattt gaaattaaac cgtaaactta aaagcaaatc
    22561 tataaaacag aatttggagt aaaaatatat gaaatcattc atgatcaaaa tgacggcgct
    22621 tttggcctgt gttgccacgg tcatcacggc gacgttgtat ttggccgaag ccaatgatgg
    22681 caaagcggtt gcgatcgtcg tcaagtccac cggtaaagcg caagccaaac aaggtaacaa
    22741 atgggttgat gtcactaagg gatcaagact tttttcgggt gataaagtgg taaccaaaga
    22801 tgatggtttt gctgccgtga tgtttcttga tgataagagt attatcaagc ttaagcccaa
    22861 ttccaatctc gaagttaaag gtcaatatga aggtaaatcc atttcgaaaa acatcgtgat
    22921 ggacgtcggc gaacttttcg tgaaagtatc caaacaaaaa ggttctttcc aagttgccac
    22981 accgacctca gtcgcttcgg taaaaggtac ggagttttgg gtgatagaaa ataccaacgg
    23041 tacaaaagtt atcggcatcg aaggtttgat cgaagttctg aataaggcat cgaataaaaa
    23101 gcaagacgtt tcggccggta atactgctaa ttcggacaag tccggtaatt tgaacgtgga
    23161 tacaaccaag gaaaatgaaa ttcctgaaga caaagatctt gaagaaatca ttatcaaatt
    23221 tagagataat gacggcaaag aaaaagaagt taagatcaaa tactaagatt gtactttaga
    23281 agttttgtaa aaaaccgctt ggcaaaagag tcaagcggtt tttttttgac ctttctcatt
    23341 gcctatcggc gtcatttcta ttaatttgtc gtttacaaat cattctcatg cagtattaac
    23401 acttggcata cctttagaag aagagacatt tcttttttat ttcacatggc gaggagagga
    23461 tttatggcac gttggatacg tttgcttttg atgtgttgta ttgtaccaat acaagcacag
    23521 agtgactgga tcaatctagg accctatgga ggagaatacg aaactatggc catagaccaa
    23581 aagaaaccta ataatttgta tatcggaacc cgtaatggtc aaatataccg ctctacaaat
    23641 tcaggtcaga cgtggtcgtt tttgagcgcc attgacggga caataacaaa attagtagtg
    23701 gattcatcaa atggtaataa catttttgtc ggagtacatg acagaaataa ctttaatcat
    23761 gtattctatt ctaaagacgc aggtcttact tggaccggga actatgcatt aaatctgtat
    23821 aatgaggaaa tcaaagattt cgttattaat ccggcaaaca acacgcaaat gcacttagta
    23881 acggtaaacc gttcaattcc ttcaattaat ggcggaaaga gttttgttgg tagtggtcca
    23941 tacttcccgg atggttttca ttcgtttgca tatgatccgc atgatccgac gaatgtaatg
    24001 tatgcagccg gccacgataa ttctttcgat cgcgatattt ttcaaggcac acgcaatgcc
    24061 tggtccaatg ttcatacgta ccaatacatg gaaaccggat ggcgaatcgg taaagtgcat
    24121 gtggaaaaca caaacattgc ggccgtatat gtattaatag gtaatgagga tagtaccaaa
    24181 ataatgcgaa gtttagatta tggtgcaacg tgggcgattg ttttctccga tgatgtggcg
    24241 atgtaccaag ggggattcaa aatcggccaa tacgcctggc ttcatcgttc taaaggcaat
    24301 aatgagcaca ttgttgcaac acaaaatggt attctgaaaa caaacaatgg cgggttatct
    24361 tggtatcgta tgaaggatgg tctcaataat aatctatcat tggttcgtgg aggaactgta
    24421 gtacgatcgc cgcaagacac gtccattttt tattgtaatt atagcaaaaa accggcgcgt
    24481 ttatccataa caccatacgg atatgtagct aatgtcaatc agtcattggg atttactgca
    24541 caagaaataa aaaaggttca ttattctcaa aataattatg tgtgggctga gactcaaact
    24601 cgggatttga tatacacatc caattatggt caagattttt atgaaacggg aatcatgtta
    24661 ccgccggggg atgaaattat cggcggtaat ggtttttttg tgacaaaacg gggctcctac
    24721 ttacaaagtg ctggcgccgt tcttggcgct ataacctttc cttcgggaac ggattcggtg
    24781 attgcagtca tcggtagtga agttttttca tccggtcttt acgataatta cgaattcgga
    24841 ataactcaaa attcaacagg tggaacttca agtggttttt tcattgcgcg gtatgatttt
    24901 tcaacaaaca atgttatttc atacattcct acactgccgg atattgctct tacatcacca
    24961 atcacatcgt tttcattttc gtttagaagc tatcagcgcg atacggttga catctacgtt
    25021 gccgcgtcta caggcatagc aacaacatac tttgcaaatg gaactttcgg atcttggaaa
    25081 accattaaac tatttgacgg ttcattcaca atcaaaaaaa tcgaatttag agagacggaa
    25141 gcttttattt gggcaaaaga acccggtggt gaccgtttat tcatcggtca tcgtgatgat
    25201 atgagttctg atttttcgat cgaagaaatt tccggctcat ttccaagaga tgaattcgga
    25261 caatccgaaa ttcacgatat tcacgccggc gtcgatgggt attatatgat acgttttcgg
    25321 tctgactttg gagcgaatgg agcggaatat actagaccgt tatataaagc agggtttgga
    25381 aaagaacccc gatggactga gataacaacg cctgatctat caaaatttaa tatccaaaat
    25441 gttttaactg aatatcgttt tcaagaaaat gctgttgtta tttatgcggc ggctaataat
    25501 ggtatttgga cgatgacaca aacagctagt attagcatag atactctagt catattttca
    25561 agcacctctg cattgttgga ttccagcatt gctgagctca agtatgccaa taccggcacg
    25621 gctttttctt acattgaacg gtttgaaatc acggatgatg cggaaaattc gttttccatt
    25681 gcagataaaa attttgccga tggagacata ttgttcttag atatccgatc cgagacaagt
    25741 tttcctatta aattcaaacc cgtatctaaa gggttgaaaa cggccaaact aaaatcttat
    25801 tataccgttc gagcgtccaa tggatcggac agtacgatta tagccacgac acgtctggtc
    25861 ggtataggaa aaacctcaca aatcggaacg aatctgaaaa atgatacttt ggatgtcaaa
    25921 ggtgcgttag tcaatgcgtc aaaaacaact aactttcagt tgtttaattc cggtgaagat
    25981 gacatgtata tcgaagatat atatttgtca aaaaacgatg ttttcgccat tcaatcgatt
    26041 aacgaaagtg atagtacttc atttatagtt gctccgggta gggtgcttcc tgttatctta
    26101 aaatttgaac caaccgaagg taaatcctat acagatactt tgatcataga aagcacggcg
    26161 tttaaggcga ataatacgcc taacccaaaa catcgtatcg tagtgaaagg tgtcggctcc
    26221 tcgattcaag taagtaacat tgataacctg agcgcttcac tgaatcaggc attaaccatc
    26281 gaagtaagtc taagtcagat tgatgcgttg gaagtcacgg gtttgctgcg ttatcgtaaa
    26341 aatggagaag gttcgtcagg gtataagaac aaaacattga cgcctaccgt aacacaaagc
    26401 ggcaattcaa gccggtttac aacaacaata cctcaggaag atattacaca aaacgggcta
    26461 gagttttacg tagaatttac atcttcttca agtgtatcac cgatccaata tcctgaaaac
    26521 ggagaatttg aaccacaggt tgtctcagtg acgattccta atcctggatt aagtagtagc
    26581 ggttatgttt ctttgccggg cggtactgat aaaaaggctt ttcagctcat gtcctttccg
    26641 ctcaatctaa ccaataaaac agtgggcggt gcatttactc caagtaatat gggcgaagcc
    26701 ggtgtcaaag gtgattggca agtatatcgc tatgataacg acctttctaa atttatctct
    26761 tcttcagacc ctgatattac atttggaaat atcgagtcag gaaaatccta tttattggtt
    26821 tctcgtaaac ctaaaaccat caatagcgga gcagggaaaa cagtaaaaaa agaggatgcc
    26881 catgtaacga tccaacccgg atggaacatg atcgcgaatc cgtatgcgtt tagtattaac
    26941 tggtctgacg ttgctaactt taacgacaat gtggaattaa ccaatattgt agttttacaa
    27001 aatggtcgtt tcaaatatgt ggatgaagtg gatgtgtaca atctcaaatt agagccgtgg
    27061 aaaggataca tgtattattc tcccgaagaa aacggcactt ttacaatcaa ttatcccgcg
    27121 atcaattcgg acaatgcatc ggctttgcag aaaaaagaga ttcgaacaac ggttgataaa
    27181 agcggttatc gttttctact aacattacat gatgacggcg aagatatagt gtttgaagca
    27241 ggaactgcgg aagatgcgca agacggtgct gatggtcgtg ataaaagagc gctccctatg
    27301 ctgatgaaag atgaggtatc gcttcactgg ttgcaagcgg atcggaaatt gcaaacggat
    27361 atgcgcgccg tttctccgga aggtcatgta tatacgtttg aggtagctac gggcgttgat
    27421 cgtcgcacgg tatcattatc ctattcagcg acaaatttgc ctgaaggttt ttcggtacgt
    27481 atggtcgagg tgactaccgg tatggtacaa acaggtgaaa ctctgaacct aatggtgcaa
    27541 aacggaaaac caaagatgta taaactactg ataggtacgg aggcttttat tgcaaaagca
    27601 caatctgaga ttatcccatc acaattttca ctggctcaaa attatcctaa tccatttaat
    27661 ccgagcacca ctttacgatt tggtttgcct gaggcgtcct cagtaaaaat tacgatctac
    27721 aatatgttgg gtcagcaggt gcgtacattg gtcaaacaag tgatgcctgc ggcatcgcat
    27781 gcgttgttat gggatgggac caatgatttc ggtcaacgcg tggccagcgg tgtatatgtg
    27841 tatcgtttag aagcaaccgg gctgggaagt aacaaggtgt tttcacagac ccgtaaaatg
    27901 actttgatta aataaaataa attaaggata gatttatgaa gaggtttaaa aaaatcatag
    27961 ctgtcttgtt atcggctgct ttttggaata ttcaggcggc attgctgttt tcgcattcta
    28021 tacacgcaca acagggcacc aggccattag tggcagttct tgatctacag gttaccggag
    28081 gtattctcaa atcggaagcg caatcgcttt ccgaccgttt acgtggcgag atgcaaggac
    28141 taaagaaatt tgatatcatc gaacgttctc agatggatgc tttgcttaag gaacaagatt
    28201 ttgcgatgtc tgatatgacg gaagccaatg cggctaaagc cgggaagctg ctcagcgtcg
    28261 aacaaatcgt agtgggttcc atcggtcgtg ttggtcgttc atatacggtg gacgtacgac
    28321 tgattgatgt tgtatccggt cgcgtcatca cggcttcgca gcaggattat aatggtccta
    28381 ccgaaggctt gacacaggtc attcgcaatg tcgcacgaca gtttgccggg cttccgcctt
    28441 tgaaagtaag cgttgcgagt aataccaaat ggtgggtgat gggcagttta cttgctgttg
    28501 gcggcggtgt ggcggcttat atgatgttga ataaaaaaga aagcggatcc tccggccctc
    28561 cggctttcag cgatccaccg gcattgccgt aatgtcttct tgtgaaataa aaaacctcga
    28621 ctgaaatcga ggttttttat ttacataaac acaaattgat cagaaagcta attggattgg
    28681 taaacgaatt cagatggtgt taaaccggtt tgctctttga atatgcgatt aaaagagctt
    28741 ttacctgaga accctgaatc taaagctata gccagcagag tttcttcgca gtatcgttct
    28801 ctgagttggt tttgggcatc ttttacacga tacgtattga caaacacgtt aaacgtcatc
    28861 ccgatgtctt cgcttagcag tgcagacaaa catcgtgcgc tgataccaag gcgcgccgcc
    28921 acttcggtat aacgcaggtc gggttctcga tatattttgt cctgttccat cagtttgtat
    28981 aattgattta aaacttcatg ctttctctct ttagaaaggc tgttattgtt tctcggccat
    29041 aaagtaaaaa aaatcgtttc cggagaaacg aatgaaagga atgcaatagt gtaaataatc
    29101 gttgcaaaaa tcggtattga ccaattaccc caatggacaa gcgtttctcc ggtgaaaaga
    29161 aagtaaatag catttataaa attgaaaacg acatatccca tcataatgct aaataagaat
    29221 cggataatgc gattgagttt agaatacgct tcattttgtg acagcaaacg aatggccata
    29281 gtgaggtaca tgacattttg caaaaccatt acgacattta gaataatact ctctaaggcg
    29341 aaatcattga actgtgacgg atcaattatg tatgcgattt tcttttgtac atcccacaaa
    29401 tagtatgggg ataagagaag aatcactccc aaaaaaggta atgcatgcca agcgtctttg
    29461 ataacgaatt gattttgctt ctcgacaaga tttttggcat aaagataata agccggagcc
    29521 agtgcatacc aaaaagggac cgaaatgaag agcatatggg gcagggcgga aaaaagaccg
    29581 gagatgcgaa tcaattgttc caatacggca ctagaaaaaa taagcatcag gagcgcaagg
    29641 cttttatttg ccagaacgct cccttttttg ttaaaaaaaa gaacgatggc aagaaaaatt
    29701 ccttggccga atatgagcgt gtatatgatg gtccaaatca tagattaaga actgtatgat
    29761 aataattcag aaaattagat tcttatacga ttaaacaatg tttttagtta caacaagttt
    29821 ctctttttat ttcgttatat aatttttatt ctacagacaa ccaaatacaa ttattatcta
    29881 tccgtttcta aaaatgaata tttgtccgat ttgtggccac agtacacatc aaaaatggcc
    29941 gatgtattac gaatttgaat cccaatgttt cgatcttttg cagtgtatat catgccgcat
    30001 tggatttgta tcgcctcaac ctgacgaaaa aacgattgta cgtatgtaca gtaaggatta
    30061 tttcgaatcg gattttagat gcgggcatga agatgcatca gcctttaata aaaagacgga
    30121 tcctgttctg ccggattgga ctgagcaggt tcaccttcaa cctgagtcat ccgttttaga
    30181 aattggttgt gcgacaggtt atacgcttaa agcatttcat gatgcggggc atcatgtcaa
    30241 agggattgag atatccgaag atgcagcccg tcatgcgcgg gatgtgtatc gcttggatgt
    30301 agatacgatg gccgttcatc aagcgcagtt ttcgtcggag aaatttgatc ttatctattt
    30361 gttggatgtg tttgaacatt tgacggatcc tgttttactc ctcaagcagg cgtatcaatg
    30421 gctgaaaccg ggtggaacac tggtgatcgt tattcctacg caaaccaata cacttttttc
    30481 gcgatttggc atgaccttat tttcgttagc cggcaaacgg acgcggatac atatgccgcc
    30541 gtatcatctt tttgaatacc gaccttcatc ggttcgacgt ttgttgctta accacggttt
    30601 ttctaaaata gatatgaaac cggtgattat gaaacccggt gaaattggtt tgcgcagcag
    30661 cgttgttcaa aatacagcca agtatgtttt tcaatgtttg aattaccctc taacgaagtt
    30721 gtcgggacgc tttggcgatc ggttgaccat cattgcagtc aaatcttgat tatctacctt
    30781 ccattcgata gttttgccta cctgtcataa tgctttctta tagtatagta tagtcggaga
    30841 atcatgaaat cgttgatcac acttaaggtc aatggcgacc tttacgaaat tgccgtcaaa
    30901 tccaatcgca ctttgcttga agcgctgcgc tacgattgcc atcttaccgg ttccaaacaa
    30961 ggttgtgata tgggcgactg cggcgcgtgc acggtgttaa ttgataacgt ccctatccag
    31021 gcctgcatca cacttgcggt agaatgcgtc ggcaaagaaa tcatcaccgt cgaaggaatc
    31081 gctaaaggaa cagaagcgca tccgctacag acggcgtgga ataatcaagg tgcatcccag
    31141 tgcggatatt gtacgcccgg ttttatggtt gtggccaagt ggcttttgga aaaaaatcct
    31201 aatccaagcg atgaggaaat caaagaggcc ttatccggca atatttgccg ttgtacgggt
    31261 tatactaaga tcataaccgc catgaaagag gccgcggcga taatgcaaaa agaaaaagcc
    31321 gtttaatcgc gatttgcgtt ataaaaattt aaaaattatt tgattgatta ccgaaagaaa
    31381 tcataatgat aaagcctaat ccgaaaaaaa atcaattttc tattatcggc aaacccaatc
    31441 cgaaaattga aggattggag aaggccaacg gatccgctct gtatactgat gatctgatgc
    31501 ttcccaatat gctcatcgga aaaattttac gcagcccgca tccgcatgcc ctgatcaaaa
    31561 aaattgatac ttctaaggcg gaggcattac ccggcgtgaa agcggtaatg atcgggacgg
    31621 aacttacgga aaaatacggc gttcttcctg cttcgcagga tgaaaccgta ctggctgttg
    31681 ataaagtctg ctatatcggt gacggtgtgg cggccgtagc ggcactggat gaagagacgg
    31741 cggaaaaagc gtgcgaactt atcgaagtcg aatatgaagt tttgaaaccg tgtcttacga
    31801 tcgaagatgg ttttcgtgaa gatcttccta aaatacaccc gcacaccaaa caagccaatg
    31861 taatgaagga agtgcatcaa gagttcggtg atgtggaaga gggtttcaaa gaagccgatt
    31921 tggttatgga aaacgaatat ttctttgaag gcaatacgca cttacctttg gaaccgcatt
    31981 gcgctctggc tcaatacgga agcgacggta aactgactat atggtcttcg acgcagacac
    32041 cgcattatct gcatgtagcc ttagccaaag tgcttcaaat gaagcgttcg catattcgtg
    32101 tcatcaagcc gcatatcggt gcaggttatg gtgggaagtc cgaaccgttt gcattggaat
    32161 tttgtgcggc atggctttcc aaaaaaaccg gtcgtcccgt gaaattttta tatacccgcg
    32221 aagaagtatt ttacgcgcat cggggtcgcc aaccgatgaa gatgatactt aaaaccggcg
    32281 tcaaaaagga cggttcgatc accgcgatcc acatgcgtac atttctagac ggcggatcgt
    32341 tcggaagtta tggtcttgtg actacgtatt attccggcgt atttctgacg ttgccttaca
    32401 aagtgcctcg gtacaaattt gatgcgtatc gtatttatac caataaaccg gccggcgggc
    32461 ccaaacgcgg gcatggtgcc gtgcagcctc gttttgcgat ggaatgccaa ttagataaaa
    32521 tagccgaaaa actcggtctt gatccggtgg aaatgcgtct caaaaatagt atcgaaccca
    32581 atacgatgac gatcaattcg tttcgtgtca cctcatgcgg tatcaaagaa tgcctcactt
    32641 ctacgcgttc gcgttcggag tttgataaaa agtacgcgtc gtataaaaaa caaaacagta
    32701 aagttattcg caaaggtatc ggtattgcgg ccagcacata tatcagcgga gcgggaaaat
    32761 ccatcatttg gaatgatatg ccgcattccg gcgtgcagat caaagtggat cgtggcggcg
    32821 gtgtgacggt attttgcgga gcgtcggata taggccaggg ttcggattcg atgttagctt
    32881 atattacagc ggaagttctg ggtgtcgaaa tatcggatgt tcacgtttgt caaaccgata
    32941 cccatctgac gccggtggat ttgggcagtt attcgagccg tgtcactttt atggcgggca
    33001 atgcgtgcat acaagcggcg gaacgcatta aagaaatgat ctttgaaata gcgagccgta
    33061 aactcggtgt accagtaact gatcttgata acggtaaacg attgattttt tcaaaatcgg
    33121 atccgaataa atttatggat ttcgtcgaag ccgcaaacat ggcggaatca gcctatggta
    33181 cgatcggtgc gaccggcagc tacaagccgc ccaaaatcgg cggatcttac aaaggcgccg
    33241 gtgcaggtcc atcaccctca tacacttttt ctacgcatat cgctgaagtg tccgtggatg
    33301 tagaaaccgg tgcggtgacg gtagaaaaaa tatggtcagc ccatgattgc gggcgagcgc
    33361 tcaatcccgt gatcgttgaa ggtcagatcg aaggcagtgt ttatatgggt gtcggtgagg
    33421 cgctttttga agaacatatt tttcataaaa acggattgca tcgcaacccc agtattctgg
    33481 aatataaagt gccgacgata ttggatacgc ccgaaatcga atccattatc gtggagtcta
    33541 ttgatcccga aggcccgttc ggcgccaaag aagccggtga aggccctctc cacgcggcga
    33601 taccggcgat tgccaatgcg atctacaatg ccatcggtgt acgtctggat acgacgccat
    33661 ttacaccgga taaaatcctg aaggctttgg aagaaaaaga actcgctgaa aaaggcggta
    33721 acggtcattc caaaaaactt cgtgatatcg gcgcaaaaaa attggtatcc catatcgcaa
    33781 cgacctgaaa cataacgatt tttttgtact gcgatagacc ttatcatttt gttgtaattt
    33841 tttttacttc ctaccgattg ctatagctgt tgcatatcga gctgcatgcc tgctgtcaaa
    33901 aatcattctt ttttctaacg aagcgtgtca tattggaata cgattcgttt aaattgttta
    33961 aggagtttta tgcgtgacta tgtattattt gccgtattag cgggattagc atgggggctg
    34021 ggagggtatt ttgaaaaatc aggattgcgt gcgatgggaa taccaccgat tgcagggatt
    34081 actttgcgca ccggagtagc gttcatttta ttagcgttat tatccatacc ggcatggaaa
    34141 cagattgcca atgtaaacga tacgacggcg tgggttatga tcatcatcgg tggcggcgta
    34201 gtagccggaa gtcttggcat gtggagtttt tacaaatcct tatccacgac ggaaaacctc
    34261 ggagttacgt tggctttggc gttttcgatt tcaccgatcg caggcactgt gatgggatta
    34321 ttcaaaggca atcaaccgat gaactggcaa acaggtatcg gtttagtcac gattatcata
    34381 ggcatcatca ttcttcagac gtcgcatgca ccgacagcca aaccttgaat ttaattgatc
    34441 ataacagcct tcaactcaac aaaaggtgac ggcatgaaaa cattcattct gagcatgttt
    34501 ttgtttctgc aatggccatt ggtttcgcag gacagcacga tgttatcgtt ggaacgaatt
    34561 tttgattccg atgaattgca gccacgtttt tttggtccgg caaggtggtt aaaaaacggc
    34621 aaaggttata cgacgcttga agtgtcggat cgagtggaag gcaaagatat caacaagtat
    34681 gatgcggcta ccgggacgcg taccgtattg atcggcgctg aaaagctaat accgcagggg
    34741 caaacccgtc ctctcaaaat atccgattat ttctggtctt ccgatgattc acggttattg
    34801 atttttacca atacacgcaa agtttggcga tataatacgc ggggtgatta ttgggttcta
    34861 gacattgggg cgggaaaact acataagctc ggtggtgccg atgctaaaac atctaccctg
    34921 atgtttgcta aattttcccc ggacggtcag caagtggcgt atgttaggga aaataatatc
    34981 tacacagaaa acctgttaac cgggaaaatc acacaattga ctttcgacgg ttccgatgtc
    35041 atcatcaacg gaacgtttga ttgggtctat gaagaagaat ttggctgccg agacggattt
    35101 cgatggagtc ccgatagtcg tgctgttgca tattggcaat tgaatgctga tggtatcggt
    35161 gtgttttata tgattaacaa cacggattca gtttattcca aagtcattcc ggtgcagtat
    35221 cccaaagccg gatatccgaa ttcatcctgt cgtgtcggtg tagtttctgc cagcggcggt
    35281 acaacggtat ggatgcaaac gcccggcgac ccgcgggata attatattcc tcgaatggaa
    35341 tgggcgggaa catcagaagc catcgtcatg cgacacctca atcgcttgca aaatactttg
    35401 cagatcatgc tcggcgatgt aaaatccggc attgtacaaa cgatagccac cgataaagat
    35461 agcgcgtgga ttgatataga tgatgaattt aaagtactgg atggcggaaa aggattttta
    35521 tggattagtg agcgtgacgg atggcggcat gtgtataaga ccagtcgcga cggtaaaaaa
    35581 acggagcttc ttacgccggg taattttgat gtgatcaaag ttatactcgc acaagaaaaa
    35641 gaaaacatgc tttattatac cgcatctccc gaaaatgcta cgcaacgctt tttatatcgc
    35701 atgaaaatgg acggtaaagg gaaagccgaa cgcattacaa cggagaccgg aggttggcat
    35761 gattaccaga tttcgcccga tgcaaaatac gcgatacata aatattcgtc tatgcaactg
    35821 ccgcaaacag tcgaagttgt agaccttccg aaacataaaa cattacgtgc tctggaagac
    35881 aacctcgctt tgcggcaacg gttggctgcc ttaaaaataa atgtgccgga gttttttaaa
    35941 attgaagccg gtaatgagtt gatgttagac gggtggatgc tcaagccggc gtcctttgat
    36001 gcgacgaaaa aatatccgct ctttgttttt gtctatggcg aacccgccga acaaacggta
    36061 gttgaccatt ggggtgacac tgattatcta tggcatcaaa tgctcgccca acaaggatat
    36121 atcgtgatca gcttagatcc gcgtggtaca ccggcgccgc gaggcagggc ttggcgtaaa
    36181 gcgatatata aacaattggg atggcttgct ccgatggatt tagccgaagg tgtgcgtcgt
    36241 gtgacgacgt ggccttatat tgacaaatcg cgtgtgggta tctggggctg gagcggcggc
    36301 ggcgcttcga cattgcagtc aatgtttaga tttccagacg tatttcacat cggcatgtgt
    36361 gtttcacccg tgacggatca gcgaaactat gataatatat ataccgagcg ttatatgggt
    36421 tttcccaaag ataacctgga atggttctta aaagcttcgc cgatcagcta tgtacaaaat
    36481 ctgaaaggaa aacttttatt agtgcacggt acgggtgatg acaatgtaca ttatcagaat
    36541 acggaaatgc tgattaatgc gctgatcgcg gcaggcaaac cgtttgacat gatggcttat
    36601 cccaaccgaa cgcatgacat tgatgaaggt gaaggaacca cgatgcatct ttactcgttg
    36661 ttgacacggt atctcaagga acatcttaaa ccgggtcccc aataaatgta aggaacaaac
    36721 cttactactg agtaatatat tcatatgatt tcaaaacaac atgaaatgta attcttaaaa
    36781 tgtattcgtc tgaatttgaa agatcattgt tccggtgaaa acatttcggc gtgtcatcgt
    36841 atggtttgct gttatttgct gtgttacggt cgtgtccttg ttgtttgaga aaccgcgtga
    36901 tgccgcgttg gcttttttgt ttattataga tatggctacg ccggaggcct ccgaacttta
    36961 tcaagttgta acggatgata tagttgtcac acatgattct ctcgatcggg agtcaaaaaa
    37021 aacgctgtat tattctattt attcgccacc cggaaatcat aaaaaatcag cgattatcat
    37081 ggcgcatggg tttactgaaa aaggaaacaa cgatcgaagg ttacaacgta tggcgcatcg
    37141 tatggcgcgt gccggtttta ttgtaatgac gcctgagttt cctatgatgc ggcggtttcg
    37201 tataagtatg gaagatgcag acaacaccat ggaagctttt cgtatagtga agcaacgacc
    37261 ggatgttgac tcgtcgtcta tcggtttgat gggattcagt tatggtgcgg gtccggttat
    37321 tatggcgtta tctgattctt cgatcagaga tcaagtacgc tttggtgtta tttttggggg
    37381 ttattttgat attcgaaaaa cactcaaata tactttaaca ggatattaca cgaccgatac
    37441 cggtcaagaa atgcgctctg tatctaaaaa taatgaccga tggaaatttc tattgggcaa
    37501 tataccggaa agtgggaacg aaaaatcgct ggttattttt aaatctataa tcgctcaaaa
    37561 aataacggat gccggatatg accccgggaa aaattttctt tcattggata gcacgcatca
    37621 atcactgtat cgatttatag aaaatcagaa tccctcggct tttgatgtgt tgtacgcagc
    37681 tttacccaac gattttcgtt ctaaattaga attggctgat cttacgtcgt atgcgcactt
    37741 aattcactcc tacatgatca tcggccacag tgatcaggat cgtgtcgtcc cgtacaccga
    37801 gagtttagca ttagcaaaaa atctcacttc ggcgcctgct ccttttgtag ctgtcacaaa
    37861 tttatttaca cacgtcaatt taaaaatcga atggggttct ttaaccgtaa tttttactga
    37921 aacgattccg ggattttttc gattatggaa aatgacgtat catattatgc gtatgcgtca
    37981 tataccggta gctattgcat agcccatcag ataatttaaa acagatgatg attgaaacgg
    38041 caaatataat ttcactcagg cggatttttg ttttgagttg ttttttcgca accacgatct
    38101 gtgggcaaaa tttgtatgag aatctgcaag tcagctcagg tgacacaccg gtggatgtat
    38161 cacgtcgcac caaagggcgt cgttttacgc ctataccctt ggtgaattat acatccgatg
    38221 acggcgcggg ttatggtctc cgcctgtgcg tgtatgatta tgacagcgtt acggtgccgt
    38281 attgtcgtgc tttgaaagcg cagtatttta ttacgacaga gggaaaatgg gcgcatgtgt
    38341 tgtctttgga ttatccgaat tttcttaaag gtcaccgttt tgattttgag ttagcttacg
    38401 ataagctcga gtttgccaat tactacggag aactttccaa tcgtatggtg gatagtttgc
    38461 atttgagtaa atcacaacga acgtttcgtc aagctacacc gagcgcatcc ctaacatgga
    38521 tttatgattt gaatgggcca tggcgcttgc gcggcggtgt gagtattcgc catacttcgg
    38581 tcaagcccaa tgctgattcc ggtaatatcc ttaaggagct gaagccggtg ggttataaag
    38641 gtggtgtatt gtggaaattg gacgctgcca tgcgatatga tacacgcgat aattatatca
    38701 attccacgtg cgggatgtta gaagagctac ttgtggaata taaaatagga ggagcgggcg
    38761 attatcaggg gtggctcgcg agttatgaac atcggcattt cattcccatc acaccaaata
    38821 tcgtggctgg atatcgttta gcggcagatc aagtctttgg tcagatacca ttttatgacg
    38881 aattgaagct gggtggtgaa ggctcggtgc gcggtatgcc gtcggcaagg cgacgcgggc
    38941 aaggtcgttt tttggttaat accgaattgc gttggcatgg aatacgtctg tcggatcgcc
    39001 gtaatatgat gcttggggtt atactattca ccgatgcggg gcagatttat aaacggaaag
    39061 acgggccatc catgactcca aaaaattggc atgccggata cggcggagga cttcgatttt
    39121 attggcatag cacgattata cgagctgatt atggcatttc cgaaggcgga agcggtttat
    39181 acattttatt ttcgcaggta ttttgatttg atttacaaat cactatagtc ctcattaaaa
    39241 caaaaaacgg cgagtttagc tcgccgtttc atatttactt ttttcggtat tacatatttg
    39301 cgatgatcgc atcaccgaat tcgctgcact tgatttcttt tgcgccatcc atcaggcggg
    39361 caaaatcata ggtcaccgtt tttttggcga tagaagtttc aagtcctttg atcaaaagat
    39421 cggcaacttc attccaaccc atatagcgaa acatcatttc gccggacaat atcaccgaac
    39481 cgggattgac tttatctaaa tcggcgtatt taggcgctgt gccgtgtgta gcctcaaata
    39541 cggcatgacc ggtgatataa ttgatattgc cgcccggagc tataccgatg ccgcctactt
    39601 gtgcggccaa ggcatccgac agataatcgc cgttgaggtt catggtggca atgacatcaa
    39661 aatcttccgg acgcgtcagt acttgctgta gtgtgatgtc ggcgatagcg tctttgatga
    39721 taacaccatt aggcaatttg acccacggcc cgccgtcaat ttccacacct ccgaattcgc
    39781 gtttggctaa ttcataaccc caatcacgaa atgcaccttc ggtgtatttc atgatattgc
    39841 ctttgtgcac aagtgtcagg ctcttgcgtt tttgtgcgat ggcatattgg atggcggagc
    39901 gaatcaagcg ttcggaacct tcgcgtgata ccggtttgat accgataccg ctcgtttcag
    39961 gaaatcgtac ttttttaacg cccatctcat tttgtagcca ggcgattact tttttagctt
    40021 ccggagatcc ttctttccat tcgataccgg cgtagatgtc ttccgtattt tcgcggaaaa
    40081 tcaccatatc cactttttcg ggccgtttga caggagaagg aacaccgtta aaataacgca
    40141 cggggcgtaa acagacatat agatcgagtg tttggcgcaa ggctacattg agactgcgaa
    40201 tgcctccgcc caccggtgtc gtgagaggac ccttgatcga aacgagatac tctttgcaag
    40261 ccgtgatggt atcatcggga agccaagtat tgtattgatc aaatgatttt tgtccggcat
    40321 atacttcaaa ccaatgaatt tttttctttc cgccataagc tttttgtaca gcggcatcaa
    40381 atacacgtac cgatgcacgc caaatgtccc gaccggtacc atcaccttcg ataaacggta
    40441 tgaccggatt atcgggtact tggagtacac cgttttggat tgaaattttt tgaccttctt
    40501 tggggggagt aatgtctttg tacacggttg gcttccttat cgtggtgaat gatgtgacga
    40561 aatggtttga aaatatagtt ccgataaacc gcaaactcaa gtcggtcgcg aatatcattc
    40621 aaaaaagcca acttcgatac aaagaacaat gacaaacgtc aggctcgata gagaattaca
    40681 atccgtctga ataacgatcc cggcgatcac cattgccgta gaatatcgcc atcaccataa
    40741 caaccataaa aaaagcaagt gccataatca tgtactgcat aaaatccctt tcaaaaaacg
    40801 attacaacca tcgttcaata ggattataca tgaaaaccgt gtattcggtt tgtgatagag
    40861 gtcacaggtt aatagagtgc gtcaaatatt tgacgatgtg atttatatag gtggcttgaa
    40921 attggtttga ccatttcata ttatccgtcg taaaacaatg atcttttaag aggaaagtat
    40981 gtcggtaaca tctacaggta attccgaaca acccacagcg aaatacgact tcactaaaat
    41041 cgaagccaaa tggcaagctt attgggatca gcataaaact ttccgtgtga cggaagaccc
    41101 caagtttccc aaagaaaaac gattttacgt tttggatatg tttccttatc cgtcaggcgc
    41161 gggattgcat gtaggtcatc cggagggtta tacagcgacc gatatccttg cgcgatacaa
    41221 acgcatgaaa gggttcaatg tactccatcc gatgggttgg gatgcatttg gattgccggc
    41281 cgagcagtat gcggtaaaaa ccggtgtgca cccggccatc accaccaaac aaaatataga
    41341 tacctttcgc cgtcagctca aaatgttcgg tttctcgtac gattgggatc gtgaagtcaa
    41401 tacgaccgat ccgcattatg tcaagtggac gcagtggata tttcttcaat tgtttaagaa
    41461 aaacctcgct tatatcgcag aagtaccggt caactggtgt ccggaactcg gtaccgtact
    41521 ggccaatgaa gaagtaccgg aacaaatcga taaaggtttt actgtcgtac gaaaacccat
    41581 gcgccaatgg atgctccgaa ttacggcgta tgcggatcgt ttgctggagg atcttaattt
    41641 agtggattgg cctgaaagca tcaaagaaat gcaacgcaat tggatcggtc gcagcgaagg
    41701 tgccgagatt catttccgca tcaaagatca cgacggggat atcaaagtgt ttacgacacg
    41761 accggatacg atttttggtg cgacctatat ggtgctcgca ccggaacatc cgctggttga
    41821 aaaaataaca agcgcagaac agcgtaatgc ggtggaagct tatcgcaaac aggccgcttt
    41881 gaaaagcgat atggagcgga cggagctggc caaagataaa actggggtgt ggaccggtgc
    41941 gtatgcgatc aatcctgcca acggtaatcc gataccggta tggattgccg actacgtact
    42001 gatcaactat ggtac
//
LOCUS       DAOQNQ010000003        38316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_341_length_38316_cov_6.917148, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000003 DAOQNQ010000000
VERSION     DAOQNQ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 38316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38316
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_341_length_38316_cov_6.917148"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PK754_00395"
     CDS             <1..625
                     /locus_tag="PK754_00395"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNB07992.1"
                     /translation="KIPLFPLGVVLFPGMPLPLHIFEERYKAMIAECLEQQSVFGVVY
                     YTGENFYKIGCTARIRDVLKRFDDGRMNIVTEGCDRFSIHRVTNDKPYLQGEVEYIDD
                     ISENIVEETELSRAAAQTFKDAVKLINKEDVPDLNRLDAKRISYIIASSAGFSLNERQ
                     AFLEMNSTAERLRKATSALRRLMERHKMTSDIESIISGNGYLHNSKL"
     gene            643..2250
                     /gene="murJ"
                     /locus_tag="PK754_00400"
     CDS             643..2250
                     /gene="murJ"
                     /locus_tag="PK754_00400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNB07993.1"
                     /translation="MSDLHNNGKKIFRSQQAGIAALFVTVATLLSTVLGLVRDKVMAH
                     YFGASLDTDLYNYGTRIPDALQTILIMGVTSSSFIPMFSEFLAQRGHEAANRLASSFL
                     NITLSVFIGICLLIGIGMPVICDVWLSPDLPLDQKNTIVHIARIFLASQIAFALSKIL
                     SGILQTHKHFTAYGLALLVYNPAIILGMVLFHDTMGIDSAAYGALLGSVFVVLVTLLD
                     LRGTGYQYNFQWFFDKEGVRRIFVLAIPNFLNMALLQFVILAYSKISIHMPDGSFSAF
                     NYALNFESFPVSVFGISFVTAIFPYLSENASQRNFQNFNYNIQNSLRQILFLTLPSGI
                     GMAILSYPIISLILGGGRFGSEAIEMTASTLFFYALVVPLESLWYLFARAFYAIKDTW
                     SPFWYRFIGTVINLGISYSLAQSMGPSAFSLGLLAAFLIQIVLFTFGLKRRVPEVDIR
                     SAAVTGGKLFVCALAQGAVVWLLVQWLPTLSFIHQKAQRMQDLMVVGISIFAGMIIYF
                     TLTAVFKCADFSVLQRVLNRIMKKESV"
     gene            2251..3627
                     /locus_tag="PK754_00405"
     CDS             2251..3627
                     /locus_tag="PK754_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNB07994.1"
                     /translation="MMSHVLIVDDEKNIRRTVEMILQDTYTVSTAESGREAFNILKEQ
                     PIDLVFLDLLMPEMNGIDVLKEIRTINPDTTVVMMSGHGTIENAVEAVKLGAYDFIEK
                     PLTKEKLIIAAKNALERVSLKEENRILRQEVNKRFEMVGESPGMKEIKATISKISPTN
                     ARVLILGESGTGKELVARAIHDNSPRKSKPFIKVNCAAIPEELIESELFGAVKGAFTG
                     AVADRDGKFVLADKGSIFLDEIGDMSLKVQAKVLRALQEGEFEKVGGTKTIKVDVRVI
                     AATNKDLKEEVKKGMFREDLYFRLNVVPILMPPLRQRKADVPFLIQHFVKLCAAEGGL
                     PEKTFSDEAMQTLSDYGWPGNIRELRNVVERTLILSLTDIVQREDLPRQILAAESTTA
                     VATNLNYEDGKTLKDFKEEAEKQYIIQVLQKNNWNVSRTAEELDVERTNLHKKLKQYN
                     IEMDKSEN"
     gene            3804..5060
                     /locus_tag="PK754_00410"
     CDS             3804..5060
                     /locus_tag="PK754_00410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNB07995.1"
                     /translation="MKKMFLLIAGSMLLWAGCTSAPRMRSIPEGAVIPEVKVLIARSS
                     QSVNISSKEAFAVKTDDNQDVGFISAGQNVELRFNENRVEIYDATGKSLGAYGNLIFT
                     TKSSSQRYIINNKQYRGSLWVTTQEGKILVINRLDMESYLMGVVRNEIGNLGMDQIEA
                     SKAQAVAARTYAIKNKGKYKIFDFQSDINDQVYDGAGTETEVSNQAVIETLGEVAEYD
                     SKPIDAVFYSTAGGVTTRSHWVWKSSDPKPYLNSISTKIGDKALDEASPHYRWEVKWT
                     GEEIEKIIKTNLPTVLAVVYPNEDFSKLATQRLYNLAVVSRDSSQRVQEFKIGFSKDS
                     YTVTGEQARRIMRGEKYMLYSSLFRLDVMRNADGTIQAVNCKGAGFGHGVGMCQYSAR
                     EMAKTGYSYKQILKFFYRGTDIRKMY"
     gene            complement(5986..6420)
                     /locus_tag="PK754_00415"
     CDS             complement(5986..6420)
                     /locus_tag="PK754_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07996.1"
                     /translation="MGITSIKYLKKIILLFLISSVACKDGGERVFFRSKDGKYTATMI
                     KTSHRSYYITLCEYEADTIPDLFIMPKFNKLDVMNPVMVFFISWNGDTCVVNTYANDV
                     EFSIKRSPLLIYKEYCTPTSNDYVEFHRLKKLNSENYIYIDE"
     gene            complement(6765..7526)
                     /locus_tag="PK754_00420"
     CDS             complement(6765..7526)
                     /locus_tag="PK754_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07997.1"
                     /translation="MCKESLLLIILILIGCSKTPKQNVPTAFNDSLLAATSIEDSIQD
                     VADTLTRFDGLLPAFFVPVGKYGYGLPNLIGGKRVLPSYKNYLSMSDTIDLSKIRYIL
                     KQDSVLIIVADISGLRTGLILALKRTSKDYLLLSAEEIGSVKTWSLSNSINSSMLFEV
                     TSYPYSIMCEMMQGYHVFKLDNRFGLIRVFDGVLFMTYSEKSELCPENTSYVQEFELS
                     QSGNKILLRALKLDEKGLKTEKRYVLGTDKFYPIQ"
     gene            complement(7528..8739)
                     /locus_tag="PK754_00425"
     CDS             complement(7528..8739)
                     /locus_tag="PK754_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNB07998.1"
                     /translation="MTLDKNKGITSIEYNYLNLPTKVEFGSSSNRIEWKYAVTGAKLR
                     KTVYTNGTVTSTIDYVSGFVYTSNNLNYTLDYFFTETGRIKRTGTGDLQYEYDIKDHL
                     GNTRISFADLLPTPNQQPDILMESHYYAFGMRIEGLGSKSDNKYLYNGKELTEDFGLN
                     WYEYGWRMYDPQLGRWHAVDPADEYNTPYVFVGNMPTIKIDPDGSFDIFYFEIDGSVT
                     VVPAPGPPRYFVRVPVFFEIAGQKWGDYMWTEYFINDDLLAFARGIYAEFGGRPQIEK
                     ETAAEIIDNRKESETYKHTGNTYKGVLEDRKDGVQFSSTIKGNGRYDHYTNPYKITND
                     DPREFQQFLASVSAAVKIAFGNYNRTNGSLFFYSPLRMEPPFSVPGWVNGKTETTPPG
                     VDPIYMRTYKK"
     gene            complement(8833..9012)
                     /locus_tag="PK754_00430"
     CDS             complement(8833..9012)
                     /locus_tag="PK754_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB07999.1"
                     /translation="MKNFDCVEMKHKAQQIIHQRLEGMSADEKMKYWQEREEDLRKRI
                     ELAKAKKSEEIIISR"
     gene            complement(8999..9463)
                     /locus_tag="PK754_00435"
     CDS             complement(8999..9463)
                     /locus_tag="PK754_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009149567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNB08000.1"
                     /translation="MKNNRVYVDTSVFGGMFDPEFERASKTFFEQIKEQRFSLVISPT
                     VADEISLAPENVKMFFEEMLAYASMLTLNPEATQLQKAYLNAGILSKKSEEDALHVAL
                     ASVSECSMIISWNFKHIVHFEKIPMYNAVNILNGYGPIIIFSPMEVIENEEF"
     gene            complement(9888..10235)
                     /locus_tag="PK754_00440"
     CDS             complement(9888..10235)
                     /locus_tag="PK754_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08001.1"
                     /translation="MTIFIEAFFLKKMIGISQKQSVIMSSVGNICSGVIGTAVMAFTM
                     IPVTGYGPHFLSDLNSKANWILSFVVMFAGSALIEITVVRLIWKQSIGKTSAALLIGN
                     AITYITIYFSIQF"
     gene            complement(10313..12865)
                     /locus_tag="PK754_00445"
     CDS             complement(10313..12865)
                     /locus_tag="PK754_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNB08002.1"
                     /translation="MQSLVDTYVIAGNGSATGKKFYEERSSLNYSTNHGYTANQAFPD
                     LASCDVWTVTYYDDYNFDNTGGDDYTFASDIEFPNNSKFDRLKGKVTGAKTKILEGVQ
                     GYAGFASAPDTVVLKNQAQSSGNISAKEIGLEGEVMLTGEMTLEAKKDAVKQPSSYAA
                     ALNGVTWLSSVSFYDKYGRVIQTQSNNSLGGTDVSNTEYDFPGKVLKTKTVHTTSITT
                     VTVKKRFDYDDGGRLIKTYQQNNTDSEVMLAKNVYNELGTLIEKDLHSTNSGSTFLQA
                     VNYKYNIRGWMTKINDPANLGTYLFGMELKYESPDAAIAGNAQYNGNISQQIWSTSLT
                     SRHAYGYIYDKLNQLSEAQYESYSGATWNQTPGIYNETMTYDRNGNIKSLLRAGKTNW
                     SSNTYGTMDNLQYGYIGNRLYSVNDAVADIANNQKYDFSDNGNELTFSASNASTHEYS
                     YDVNGNMKEDKNKGITNIEYNYLNLPTKVEFGADHQNRIEWTYTVSGVKLEKRVYTSG
                     ALTLTQDYVSGFVYKNGVLEYFSNTHGRLKRKPNGSLRYEYMLSDQVGNTRVTFTDDD
                     NNGQPEIQEESHYYPFGMRIEGISSNALDNKLTFNRKELEDDLGLNWYHYGARFYDPQ
                     IGRWHVVDPSDEFHSPYLALSNNPIITVDPDGRDVIYLLDQNAPFGTGLSGHTALLIG
                     NDETGWYLFSKNGFVDGKESNTDGQYFSNLAAFEKEGYWGYEKGAYVQTTHEQDQLLL
                     ASARRNVTKKYDTQDNNCDGFCRSTLSENRMKTAGSEKFLGIIWPKEGFEDFVKKMSA
                     QVLNLHMPDNYSPIVGHNFVVRDREGIVESGPFRTNQWYDPNTDTWKDWGTK"
     gene            complement(13213..13896)
                     /locus_tag="PK754_00450"
     CDS             complement(13213..13896)
                     /locus_tag="PK754_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNB08003.1"
                     /translation="MVKHLSLNKKILYLFFLGNIDVLINHFYGPKFYHEHPEHIFAFQ
                     ITLLVIPFIVLIYAIKNTSLTWKSIGITTTIGNIKKSSAKYYYVFSFFILILLVEAIS
                     TSIVKFLIPINLLAMNPVEIPKKSVFVDFLVGFLIAIRSGFFEEIYFRGFLNLFFANK
                     ITFVIISSLLFGFMHWPMGLHSVVTSVLIGIVYSINYIRTGSLVPSIIAHTVYDLLLE
                     MGKVFLGAA"
     gene            complement(13890..14267)
                     /locus_tag="PK754_00455"
     CDS             complement(13890..14267)
                     /locus_tag="PK754_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08004.1"
                     /translation="MKSIGLRVQSNDNGSINFSMRLGNGATVHSPGGQGVNIMKNDKS
                     IIKDFGSGFNYTVKLQYKPSLGLMIMEDLFQKYLERSGLKVPLTVKKDPFVPRESFTP
                     KNYVKPVTPETIRNQMTSPGLTW"
     gene            complement(14411..15127)
                     /locus_tag="PK754_00460"
     CDS             complement(14411..15127)
                     /locus_tag="PK754_00460"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HNB08005.1"
                     /translation="MEREEISRMLAQDFSFRQMTMRLSSETIYTYIYILPRGTLKKEL
                     ISCLRQNRKRRYRHTRGAKPVRNLEDMLSIEERPQSLEDRIIPGHWEGDLIVGKYNRS
                     ALGTLVERTTRTTILVPLKNRNAYDLAKAFAKEVIKLPQQMKLSLTYDQGREMAEHKL
                     FTHITGMKVYFAHPRSPWERGTNENTNGLVRQYFPKGTDFNNVSRYQIKKVQNLLNGR
                     PRKVLNFQKPYEVFNQLINQ"
     gene            complement(15194..15892)
                     /locus_tag="PK754_00465"
     CDS             complement(15194..15892)
                     /locus_tag="PK754_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08006.1"
                     /translation="MQRHINGMDISMISKKLLKNTIRFTPFLLMCLLIVFSSCEEKKL
                     TINFIQDELIVNSNSDTLSTIVYLINRSNDSLIVYTAFNKTIPRPEVFGNGPLMESKY
                     DCRDVVLNGFNVLLYDSAQEMCLVRDLPYHFDIDTLLNNKAIGSNSINSKRQHESTNT
                     YDDILSSRVRLLPSDTLQVSIQIPLNRYSLSNGGYWLRLIYFVNTNSFPHSLYDSNVF
                     DGCVFSKMIKVFIR"
     gene            complement(15825..16379)
                     /locus_tag="PK754_00470"
     CDS             complement(15825..16379)
                     /locus_tag="PK754_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08007.1"
                     /translation="MLRVKELLKQVILIPKNLLKLAKKTSKAIKNSEELQKLHKAVGN
                     SDVRITTPENLKAVGSRRIGVIDGSMYDLKDGQMSAGYTGVIQRSGFKTSDGSIGFRT
                     YNIISRSAFASAEKLFLTLGHELTHSIHYFSGEYVRWGNSQNSDDAHNMSEKAAYAWE
                     LNYATSHKWDGYINDIQKALEEYD"
     gene            complement(16599..17228)
                     /locus_tag="PK754_00475"
     CDS             complement(16599..17228)
                     /locus_tag="PK754_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08008.1"
                     /translation="MKQKTFKLMQLFSIAASFYFITGCVGISNSRFSKLDLENNNFNK
                     IVEEPYVGEMIVEKNFAYRVFPLNAADSGFLIFPIPFPFSESTPIDSEFSVGISLKPF
                     CTDLRFDPQKVIFWTDSSTKYLPYGFIGPYECSSSDTRPIERKLPVEPIMLEDSITSC
                     FWIKFKTIVPDPKKKLHFEIKGLSINGSEVVIPEIVFKQAKRRELFSLP"
     gene            complement(17463..17888)
                     /locus_tag="PK754_00480"
     CDS             complement(17463..17888)
                     /locus_tag="PK754_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08009.1"
                     /translation="MKILIVIVLLRILSCDEEKNSYSTMGRNHYIFEFPGKIYGRTII
                     GFIETYDSIAIKTSFEISYSKDRIIHVNKITDSSQYKYSVNAEKNVYVHGPRITCYDT
                     NNFKNNKIHKEAFYRLIADDFLSKTNKEKHDFLLELSSK"
     gene            complement(18250..18441)
                     /locus_tag="PK754_00485"
     CDS             complement(18250..18441)
                     /locus_tag="PK754_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08010.1"
                     /translation="MSDKVICCFCGKTLPLEEATILTIQPNIKSGEKQNLFCHKNHLM
                     ERLIKSIPLHPDLFDDDDK"
     gene            complement(19103..20260)
                     /locus_tag="PK754_00490"
     CDS             complement(19103..20260)
                     /locus_tag="PK754_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNB08011.1"
                     /translation="MGRDKLFSVLRESGMLIKPKRRYVQTTDSRHWVRKYPNLISALP
                     PPTRSEQLWVSDITYVASDEGFGYLSLITDAYSKRIMGYAFRSDLTKEGPLSALRMAL
                     KGRRYTDQALIHHPDRGYPYLCASGNITSSSPKGIGTLTYDPFIHRTRTIAIGASSMN
                     FQYDGTERRIYRKEVVSGVTSEALYLHDGMNAIHERMKTGTVTEYVYGPTGIIAYGSA
                     STWKYVIKDHLGSTRAVVDGTTVTGYDYSAFGSILAPVTPNTNYMYTGQEYDKTSGLH
                     NYKARMCDSDLMRFYGMDPAGQFSSPFLYVGNNPALRNDPDGKIAPLIAGLIIVGSGI
                     YNAYQNRDKIDNFGKGFSFFLVGGGQATASIFGWPTGAIIGGMLGSGANSA"
     gene            complement(20444..20848)
                     /locus_tag="PK754_00495"
     CDS             complement(20444..20848)
                     /locus_tag="PK754_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08012.1"
                     /translation="MQEGQGCRMYVKRTQRDYSLAFKLQVVDAIEKGDFTRKEALSKY
                     GIQVQSTLHVWLCKHGRLDWSSTMQKKATPGKRIRELVKALERAREDKEILQRAIDIA
                     EAQLGIDIRKKYLAQLSEAMAIKGAGDTALGE"
     gene            complement(20953..21102)
                     /locus_tag="PK754_00500"
     CDS             complement(20953..21102)
                     /locus_tag="PK754_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08013.1"
                     /translation="MKKLMLVAAAALMVTSFVFAGMGQDNCGSCPNKENCGTKVCTPK
                     ECCKK"
     gene            complement(21685..22680)
                     /locus_tag="PK754_00505"
     CDS             complement(21685..22680)
                     /locus_tag="PK754_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HNB08014.1"
                     /translation="MKTQSRWVYVFSFLYPLSVVAGNTLGGHFARSGAVVGLLIYPLL
                     DWLLGESESNNLEDSDPFYFDAILMTHAVLQIVCLLSLALLIQRDGWNYFAVLASIST
                     GLNSGISSIVIAHELIHRRYAFQRVTGVFLLWTVNYMHFYFEHVRGHHKSVATVEDPA
                     SARENENFWSFLIRTIPGQFINAFKIMSPRANSWRLNPVLVFLVIQIVSWIVVYFLLG
                     KFILFAWLIQSAFAVFLLEYVNYIRHWGLQRPVGSRVQPEHSWQSNHRWSRWTLVELT
                     RHADHHYIASREYWKLRTYRESPNLPTGYYGCFWLVVIPPIWRKVMKKRLPKQGI"
     gene            complement(22715..23878)
                     /locus_tag="PK754_00510"
     CDS             complement(22715..23878)
                     /locus_tag="PK754_00510"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNB08015.1"
                     /translation="MKRIGVLTGGGDVPGLNPCIKALVYDAIHAGMEVVGIRSGWGGL
                     LRYNPDDPEGSKKWITPLDLINTRTIDRSGGTFLHTSRTNPGKVNKSDVPDFLKKPEH
                     DTLDSKTKIDFTPHIFRVLKHLKIDALVTIGGDDTLSYSVRLHNEDFPVVAIPKTMDN
                     DVYGTDYCIGFSTAVTRSVHFINMLRTPAGSHERIAVVELFGRNSGETSLISALLANA
                     DRAIISEVPFDPEKLSEMLLNDKRNNPSNYAIMTISEGATMEGGKIIEYGQEDAYGHK
                     KLGGIGEITAEAIKKITGQHMIYQSLAYLMRSGEPDALDRMVAMNFAGLAIDLLTKNR
                     FGMMTALQDGRYTTVPAETIIQGKRNVDVKELYDVENYRPKVFNVMHKPMFLY"
     gene            complement(24022..25251)
                     /gene="xseA"
                     /locus_tag="PK754_00515"
     CDS             complement(24022..25251)
                     /gene="xseA"
                     /locus_tag="PK754_00515"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNB08016.1"
                     /translation="MDLFSVSNENSSDLGQRQEKKPVIYSITEITRLIKTHLEDSFPA
                     ITVSGEISNLTRASSGHVYFTLKDAGAQIKAVIWKSSSASQSDALKDGKKVIARGNIA
                     VYEKGGYYQITVQKIQQEGIGALQEAFEKLKQKLYAEGLFDTSHKKTIPKYIQRVGII
                     TSPTGAAIRDILSVARRRMPWIELILYPVKVQGEGAAEEIAHAIHEFEKYGAVDVLII
                     GRGGGSLEDLWAFNEEVTARAMYACSIPTISAVGHEIDISISDYVADYRAATPSAAAE
                     MVTVDQEDMRSYIVNLQYTLSQNVKKRISDYQKQLHNLKNRYAFRQPENIVGQYRQQV
                     DDFEKTMMRVLRHKLIVSGQTITALARQLQTLNPKNTLQRGYAIVRQKDQIVMRASEW
                     DDTKPTELEFFDGKKNT"
     gene            complement(25257..25916)
                     /locus_tag="PK754_00520"
     CDS             complement(25257..25916)
                     /locus_tag="PK754_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08017.1"
                     /translation="MSSAKRIGCFIFIVLFTVCGLSADAQVRRKPTKKVEPKKETKKE
                     KGKTSYLIGATTSYSGITGVVVEAVGKSNKSTYSLEAGYSFRRVKFESPYIVLDTSLT
                     NKTEMVHGFGAHICMNNYLSKYKQGFAWTLGVGGHYFFKKHDAKIYNITDEPYYRPGP
                     DVKFFSVFGRVSYRLETDEGMYLTPFIGGGIMATPFKSTVHSKINGAFFQGGLNFVMK
                     L"
     gene            complement(25913..26605)
                     /locus_tag="PK754_00525"
     CDS             complement(25913..26605)
                     /locus_tag="PK754_00525"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HNB08018.1"
                     /translation="MRKSEKRIISLCITVLFSLSFTVHAGSGDEEKKVVISSMRTANE
                     ATISLQAKMKQRKQSSFMDKEIVTKADFYYQKPGKYALNPSSEAENQYIVNNNSIWII
                     NRKNKTVTTTSDNEINFSQYLLGFGNSLEMLEKYFDVQVDTKQVQKKFGSYKLTLVPK
                     RGSKFYDKMESVIIYVRDDLWLPYQAELSEADGDKTIWEFSDFRLNHSINSDVFKQET
                     PKGFQVKNLEKK"
     gene            26882..27433
                     /locus_tag="PK754_00530"
     CDS             26882..27433
                     /locus_tag="PK754_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNB08019.1"
                     /translation="MALRKLAYDEIKQLRSTPEELVYKTRFPIYGVLENIRSMYNVGA
                     AFRTSDAACIQELILCGYTAQPPRSQIEKTALGATETVPWRHFNNTLDSINYLKDRGV
                     TVVALEHCEESKNLLTTEFPFPVALFVGNEVDGLNEETVAHCDMACEIPMFGSKQSLN
                     AAVAYGIAVFELTRKYLVLQEKG"
     gene            27438..28025
                     /locus_tag="PK754_00535"
     CDS             27438..28025
                     /locus_tag="PK754_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08020.1"
                     /translation="MRSLMVLFLFINLTIYGQEFSIGWTKASTESGSEFKKYEPQTAC
                     FFIKHRRLYNEDFMWGLGLDMMSFRKSASPERTIKIKGITGEIGYYFRQIPGLTVLGS
                     TSFGLMRCEYDFIDPPADLKGSSGTGVSIEPSVSIFFPLSKTLIFSPSIGYHMLFDKM
                     NFKTTNPIKQPIGKLDSHMFAMTYRLALSWRFRKE"
     gene            28030..29010
                     /gene="nadA"
                     /locus_tag="PK754_00540"
     CDS             28030..29010
                     /gene="nadA"
                     /locus_tag="PK754_00540"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNB08021.1"
                     /translation="MIFKKNTYSNDEINIQRISPDIDLFKEINRLRKDLNAVILAHYY
                     QESEIQDLADFVGDSLELARQAAKTKADVIVFAGVHFMAETAKILNPEKLVLLPDLKA
                     GCSLADSCPTDEFKKFREAHPDHFAITYINCTAEVKALSDLICTSSNAERLIAQIPES
                     TPILFSPDKNLGAYIRKKTGRPMILWQGSCMVHEIFSEKKLVQLRATHPEAKIIAHPE
                     CEETVLNMADFIGSTSGLLKFVQQSPDHTFIVATEAGIIHQMEKACPDKTFIAAPPNN
                     NCACNECPHMKLNTLEKLYLCMKNKTPEITLPEDVRTRALKPILRMLEMS"
     gene            complement(29041..30111)
                     /locus_tag="PK754_00545"
     CDS             complement(29041..30111)
                     /locus_tag="PK754_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB08022.1"
                     /translation="MRSDSIEMEFELLGDNGNVNIAKERIYGPIKNYTDFYKTMYRAV
                     ENLIQRINLTEKNILSPNGVLLGHERLRTIASDHEEYALYVQKWEALGYYSLGVSAFE
                     KNNDSDAILFFEKARRLDYNNLLKSEKNISAVYLRAANMAYRNSHWPSAITGYNRAIE
                     FQPENADAVFNLGNVYQARKEWDVAVLQYRRALELNSKYVDAWVNLGVVLGEKGNAPE
                     ALVAYESALKIDSLRALVHYGCAVGYDDNLDYVKAEIHYRKAIVLDSNLYDAYLNLGI
                     LLKRKKQHKEAQSLFEKSLALNPNNANIHRSLGIVYMNDKKQIKKAIHHLEQTLALDP
                     AQRDADVIQKNINVLKKRLSKK"
     gene            complement(30418..31950)
                     /gene="zwf"
                     /locus_tag="PK754_00550"
     CDS             complement(30418..31950)
                     /gene="zwf"
                     /locus_tag="PK754_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNB08023.1"
                     /translation="MAAHVTEALRQVKSDARAGTCSVIIFGGSGDLARRKILPALFNS
                     AKEGLLPEKFTVIGCGKPEMSSEAYREMVSSALREFAKSDAVDPKIVNEFVQNSFYVG
                     GGFDENALYQTMDSLIKNNCTTRACNDNRIYYLATPPTVFATICQKLAEHNMSQVRGG
                     NYWRRIIIEKPFGRDLESARALNAEVAKAFEEWQVYRIDHYLGKETVQNVLAFRFANG
                     IFEPLWNRNYIDHIQITAAEALGVEDRGGYYDNAGALRDMIQNHVLQVMALVAMEPPT
                     TFDANAVRDERVKVMRAIRPVKPDQVNDYFVRGQYGAGQINGKPVPGYRTEPKVDPQS
                     NTETYVAAKFYVDNWRWAGVPFYLRTGKRMPRRDSEIAIFFKRTPHMMFTQTAADDVA
                     PNVLVIQIQPDESITMSFEAKVPGPEMQLQSVDMHFNYKEAFQVEIAEAYQRLILDCM
                     RGDATLFMRKDMVEVAWWLVMPVLEHWKNNKAEFPNYVAGSWGPEAADKLLNKDGRKW
                     RS"
     gene            complement(32018..33196)
                     /locus_tag="PK754_00555"
     CDS             complement(32018..33196)
                     /locus_tag="PK754_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNB08024.1"
                     /translation="MRFIWFFLTWTAIIMSGYWYVGRRIVPAMAHFGLPVYIGWIVIA
                     VLCLLPLGGFMMVLQRQHESISLDVLLWCGYSSLGLFSLLLVAFLLRDAGGWLHHGFT
                     WLFTKPNETVTDETRRQFLIQSVNLGVAGFCTAVAGYGIFKAHNDPVVEAIEVPLQNL
                     PKNFDGFRIVQFSDLHVGPTIKREFVQRVTDHVMALKPDLVAFTGDLVDGTVAWLREE
                     VEPLKQLQAPHGVFFVTGNHEYYSGAKAWCDEAKRLGFDVLLNEHRLIHRENQQICLA
                     GITDYGATDFIPGHLSDPVKAIDGTSSDYSRILLAHQPKSAPAASAAGFDLQLSGHTH
                     GGQFFPGNLLAAMAQPYLKGLHRHDNMWVYVNRGTGYWGPPMRIGSPPEITVITLRCA
                     "
     gene            complement(33287..34891)
                     /locus_tag="PK754_00560"
     CDS             complement(33287..34891)
                     /locus_tag="PK754_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB08025.1"
                     /translation="MNQRSLFQIVLAVALVFTTACKKNDAVSDATGDTPQYGGTVIYG
                     KNGPPVTLDPAFVEETESSIIVGNLFEGLIIQRAGKIALDPGLAKSWKISLDGRTYTF
                     NLKTGLTFHDGTPFNAQSVIFTFERQKSGGEGESSSGYALWKNFNMDNIFQSIEAIND
                     STVKIVLQKADATFLNILSLNFLAIVSPAAVQKYGAEFSKNPVGAGPFKFVSWDTDGS
                     VRTAAFDGYWHGRPYLDTVIFRPVPDPHERWLALKSGVINMMGLPTQKDLADIEKTPG
                     VKVTKQPGMNVAYVGMNMRKKPFDNPKVREAIVLAINKERIVREVFAQLGSQAKNPVP
                     PTLLGHNDQIQAAPYDPERAKQLLKEAGFPNGFKCKLWGLPLVRDYMPNPPLAAELII
                     NDLKAVGITAEEEKISNWGEFLRRRGRGEHEMSVSGWVGDAPDPHFFFYPLLDKDITK
                     QQSSTNVSFYASEEMHNLIKRGRQTFDPNARADIYKKACEVFNKDFPWVTLAHALTIV
                     PMRDNVMGFELHASATRRFEKTWIKQ"
     gene            complement(34951..36510)
                     /locus_tag="PK754_00565"
     CDS             complement(34951..36510)
                     /locus_tag="PK754_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB08026.1"
                     /translation="MFFFVSGCSKKENVNTVSDNQPRFGGTLLYGKHGPPVTLDPAKV
                     AETESSILTDNIFETLVRLQPGNVDVVPGLAKSWIIAVDGLSYTFNLQSGVRFHDGTP
                     FDAKAVLYSLDRQKKGARRSADQFSNWINFNMDELIREVRAVNDSTVQFKLHRADATF
                     LKILSMQFLAIVSPTAVEKYGADFEKHPVGTGAFMMSASSDWNDLSLVANPNYWGERP
                     YLDTIRFLSEPDPEKRITRLEQGEFHLIEPPPFDMLARVKRNEQIIQFKQPGANVSFL
                     AMNMSKKPMSDIRVRKAVAYAINREKLVQAVYGSFGVPAKNPIPPGMLGYNDDLRPTP
                     YNPAESERLLKEAGYKRNLKLKLWNVPLPREYMPHPEKAVEMIRSELEKVGIEIEVVT
                     PPWETYIQDTYLGDYDLLIGGWIADIPDPDNFFYPLLSKTTVRQKGTGNSAFYDNDEM
                     HRVLLKAKAATKTEERDQLYKNACQIFQRDVPWFTIAHTQIVIPRRREVMNFNPYASY
                     TRNLKNVWVKS"
     gene            complement(36573..>38316)
                     /locus_tag="PK754_00570"
     CDS             complement(36573..>38316)
                     /locus_tag="PK754_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08027.1"
                     /translation="MYAFGKLLGAVKNNVEVDEAVVEMFCREIKLDIETINSDKVMQN
                     LPAQYRARVPDLEVRFVFNRMMSNTASLEGFERVHDLFRAYLTDQTDFPLIHEMFIER
                     AAEKRHASALMSFYVAALLVHELGDIVSDQLIKVLAGRLSLTYSFTRRLTLFQEIKKL
                     RYEFDGSHTGRETLYYNGFDKQNAVVPEKEKQTPLKSRPFEEQWIILYHYLNNRELAQ
                     ALRLLDDQILFDYIYKREHTQEEIDVARTELRQKVYKIIDDKFLDVVSNEEDREKREL
                     ESEGKFAAGYYGKYEKVRSILVAQFVASGWDMEVHELRRKGKRPLIKMTQTELLRGIK
                     NIDPNRILEQYEYKEARENIDVSTLRKQVDARELLLGFVNRTIKDETAFYQKALGLSD
                     EAVALLSYSSTKLRSNELRVELGVRQLVERLRADLGVAAQREPLPLHDILQKAFAGTY
                     GKIDVKVIKKDKNGKPMIDPETGEEAEKTYTIEPGKFPDYLKSARNEIEALGKSELYR
                     QFYLITLSSILSETFSSMTFIKEALATNKYVIAADGLKEQSLENQEIIEYARIKMDSA
                     AQLMPFELQLQPIS"
BASE COUNT        10922 a         8572 c         7567 g        11255 t
ORIGIN      
        1 taaaataccg ctgtttccgc tcggcgtcgt tctttttccg ggtatgccgt tgccgttgca
       61 catttttgaa gagcgttaca aagcgatgat cgccgaatgt ttggaacagc aatcggtttt
      121 cggtgtggtg tactacaccg gagaaaattt ttataagatc ggttgcaccg cccgtatccg
      181 cgacgtactc aaacgttttg acgacggacg catgaatatc gtcaccgaag ggtgcgaccg
      241 gttttcgatc catcgcgtga ccaacgataa accttacctg caaggcgaag tggagtacat
      301 tgacgacatc tcggaaaata tcgtcgaaga aaccgaattg tcacgggcgg cggcgcaaac
      361 atttaaagac gctgtaaaat tgatcaataa agaagacgtg cccgatctca atcggctgga
      421 tgccaaacgt atctcttata tcatcgcctc cagcgccgga tttagtttaa acgagcgcca
      481 ggcttttttg gaaatgaatt ctaccgctga gcgtcttcgc aaagccacgt ccgccttacg
      541 ccggttgatg gagcgtcaca aaatgaccag cgacatcgaa agcatcattt ccggcaacgg
      601 ttatttgcat aattctaaat tataatacgc ccggagtcac ccgtgtccga tcttcacaat
      661 aacggaaaaa aaatatttcg ctcgcagcag gccggtatcg cggcgctttt tgtaaccgta
      721 gctacgctcc tgagcacggt cctcggtctt gtacgcgata aagtgatggc gcattatttc
      781 ggcgcaagtt tggacaccga tttgtataac tacggcacgc gtatcccgga tgctttgcag
      841 accatattga ttatgggcgt tacgtcgtcg tcattcatac cgatgtttag cgaatttctt
      901 gcccagagag gccatgaagc cgccaatcgt ctggccagca gttttctcaa tattacactg
      961 tccgtattta tcggcatctg tttactcatc ggtatcggta tgccggtgat atgtgatgta
     1021 tggctctcac cggatttgcc cctcgaccaa aaaaacacga tcgttcacat agcccgtatc
     1081 tttcttgcat cgcagatcgc ttttgcatta agcaaaatcc tgagcggtat tttacaaacg
     1141 cacaaacatt ttacggcgta cggattggcg cttttggttt acaatccggc tatcattttg
     1201 ggtatggttc tttttcacga tacgatgggt attgattcgg cggcctacgg tgcgcttctc
     1261 ggttcggtat ttgtcgtttt ggtgacgctg cttgatctgc gcggcaccgg atatcaatat
     1321 aactttcagt ggtttttcga caaagaaggg gttcgtcgta tttttgtttt ggctataccc
     1381 aattttctaa acatggcgct gcttcagttt gtcatactgg cgtattccaa aatttccatt
     1441 cacatgccgg atggcagttt tagtgccttt aattacgcgc tcaattttga aagttttccc
     1501 gtaagcgttt ttggaatttc ttttgttacg gcgatttttc cgtatttgtc ggaaaacgcc
     1561 agtcagcgta attttcaaaa ttttaactac aacattcaaa attcactccg ccaaatattg
     1621 tttttgactt tgccgtcagg tatcggcatg gctatcctaa gttaccctat catcagtctg
     1681 atattgggcg gcggacgatt tggttcggaa gcgattgaga tgaccgcttc cacgttattt
     1741 ttttacgcat tagttgttcc gctggaaagt ttatggtatc ttttcgcacg tgctttttat
     1801 gcgatcaaag atacgtggag tcctttttgg taccggttta tcggtacggt gatcaatctc
     1861 ggtattagtt attcgttagc ccaatcgatg ggaccgtcgg ctttttcact ggggctactc
     1921 gcggcgtttc ttattcagat tgttcttttt acgtttggtc tcaagcggcg cgttcccgaa
     1981 gtggatatcc gaagcgcggc ggttaccggc ggcaaactct ttgtttgtgc gctcgcgcag
     2041 ggtgcggtcg tctggttgct cgtgcagtgg ttgccgacat taagttttat ccatcaaaaa
     2101 gcgcagcgta tgcaagacct tatggtggta ggtatcagca tcttcgccgg tatgatcatt
     2161 tattttaccc tgacggctgt ttttaagtgc gctgactttt ccgtattgca aagggtgctc
     2221 aatcgaatta tgaaaaaaga gagtgtttag atcatgagtc atgttttgat cgtggacgac
     2281 gaaaaaaata ttcgtcgaac cgtagagatg attttacaag atacttatac tgtctcgaca
     2341 gccgaaagcg gacgggaggc ttttaatatc ttaaaagagc aaccgattga cctcgttttt
     2401 ttagatctat taatgccgga gatgaatggg attgacgtgc tcaaagagat acgcacgatc
     2461 aatcccgata cgacagtcgt catgatgtcg ggtcacggca ccatcgaaaa cgccgtggag
     2521 gccgtcaaac tcggcgcgta tgattttatc gaaaaaccgc tgaccaaaga aaaactcatc
     2581 atcgcggcca aaaacgcgct cgagcgcgtt tcgctcaaag aagaaaaccg aattctgcgt
     2641 caggaagtga ataaacgttt tgaaatggtc ggcgaaagcc cgggcatgaa agaaatcaaa
     2701 gccacgattt ccaaaatttc cccgaccaat gcccgcgtat tgattttagg tgaaagcggc
     2761 accggtaaag aattggtcgc ccgcgccatc catgataaca gcccgcgcaa atccaaaccg
     2821 tttatcaaag tcaactgcgc tgcgattccc gaagaactta ttgaaagcga attatttggc
     2881 gccgtcaaag gcgcattcac cggcgccgta gccgatcgcg acggcaagtt tgttttggcc
     2941 gacaagggtt ctattttttt ggatgaaatc ggcgacatga gcctcaaagt gcaggccaaa
     3001 gtgcttcgtg cactgcagga gggcgaattt gaaaaagtcg gcggtaccaa aacgatcaaa
     3061 gtagatgtgc gcgttatcgc ggcaaccaat aaagacctga aagaagaagt caaaaaagga
     3121 atgttccgcg aagatcttta ttttcgtttg aatgtcgtac cgatcttgat gccgccttta
     3181 cgccaaagaa aagccgacgt cccgtttctg atccaacatt ttgtcaaact ttgtgccgcc
     3241 gaaggcggac ttccggaaaa aacattttcg gacgaagcga tgcaaacatt atcggattac
     3301 gggtggccgg gcaatatacg cgaactgcgt aatgtcgtcg aacgcacgct gatcctcagc
     3361 ctgaccgata tcgttcaacg cgaagacctc ccgcgccaaa tattggccgc cgaaagtacc
     3421 acggccgtgg cgaccaatct gaactatgaa gacggcaaaa cgctcaaaga cttcaaagaa
     3481 gaagcggaga aacaatacat catacaagta ttgcaaaaaa ataactggaa tgtatcgcgg
     3541 acggcggagg aattggatgt agaacgaacg aatctacaca aaaagctgaa gcagtataat
     3601 atagaaatgg ataaatcgga gaattgatgg tgtttttcgt tatttataat aacggtgcat
     3661 tacaaaaagt gtcgcacaaa aaaagaaaaa cacggacacc aatagtacca taagcagcaa
     3721 gtcttgatta gcagaagcca tataacgaga tcctttgtga gggtaaatta cttatacaaa
     3781 tattctaacc aagcggtcac accatgaaaa aaatgttttt attgattgcc gggagcatgc
     3841 tcctatgggc gggttgtacc tccgcacccc gtatgcgttc gataccggaa ggcgctgtaa
     3901 tacctgaagt taaagtactt attgcccgct cctcccaatc cgtgaatatc tcttccaaag
     3961 aagcttttgc cgtcaaaacg gatgacaatc aggatgtcgg atttatttca gccggtcaga
     4021 atgtggaact gcgtttcaac gaaaaccgcg tggaaatcta tgatgcgacc ggcaaatcgt
     4081 tgggtgcgta cggcaatctg atttttacga ccaagtcgtc ctcgcagcgc tacatcatca
     4141 ataacaaaca ataccgcggc agtttatggg taacgacgca agagggtaaa atattggtca
     4201 tcaaccgtct cgatatggaa tcgtacctga tgggtgtcgt acgcaatgaa atcggaaacc
     4261 tcggaatgga tcaaatcgaa gcttccaaag cgcaagccgt cgccgccaga acctatgcga
     4321 tcaaaaataa aggcaaatac aaaatttttg atttccagag cgacatcaat gatcaggtct
     4381 atgacggcgc gggtacggaa accgaagtga gcaatcaggc agtgatcgaa accctggggg
     4441 aagtggcgga atatgacagt aagccgatag acgccgtatt ttattcgaca gccggaggcg
     4501 tcactacacg ctcgcactgg gtatggaaaa gctccgatcc gaaaccatac ctcaacagca
     4561 tcagcactaa aatcggcgat aaggctttgg atgaagcctc gccgcattat cggtgggaag
     4621 tgaagtggac cggtgaagag atcgaaaaaa tcatcaaaac caatctgccc acggttttag
     4681 cggtcgtcta tcccaatgaa gattttagca aattagccac acagcgtctt tataatctgg
     4741 ctgtcgtttc gcgcgactcg tcgcagcgcg tacaggaatt caaaatcggc ttctccaaag
     4801 acagctatac cgttacgggc gagcaagcgc gtcgcatcat gcgcggtgaa aaatatatgt
     4861 tatacagctc acttttcaga ctcgatgtga tgcgcaacgc cgacggcacc atccaggcgg
     4921 tcaactgcaa gggcgccgga tttggtcacg gtgtcggtat gtgccaatac agtgcacgcg
     4981 aaatggccaa aaccggttat tcgtataaac agattctcaa attcttttat cgcggcaccg
     5041 atattcgcaa gatgtattga tggaaattct tgatcacaaa aagaccccgc actttacagt
     5101 acggggtctt tctaatttac aataactcag gttacctttt ttctaaataa aaaaatcacc
     5161 catgacgaca caagtcgaaa cttcaaaaga cctgactcta tcatatataa tatacgtatc
     5221 atgcattttt tgttagcaaa ccgaattaag aacccatcgt atttgtctga atatagattt
     5281 tagaaatgtc gctatctttt atgttaaaag gattttaaca tcaacgtcaa ctaatcgaat
     5341 cacacgctac agaaatactt attgagtctt acaatgtagc ggcttcttta aattattttc
     5401 atcaaataac agcggaggtg agattgaaaa ttgatcgtcc tgctttattc tgtatttgga
     5461 tagaaatacc cgttttcagt ataccgaacc atatggagtt gtacaaaaag gcgactaatt
     5521 agtcgttgta tcactaaatg cttaaagtta atgaaagtat ttccagtttt cttcataaac
     5581 aaaattaact ccattaacat aatacctcat cgtgcgaact tcagaagctg catagattat
     5641 tttcacggta tcaggcgaga cctttgtcac tgttaccccc cttgcgtgat atgcaaaaaa
     5701 aacgttacct gttctgtttc ccaaattttg caaatcatca tttgtcatgc aaactgagac
     5761 gttcgcatcc atatcaactg tagcaccaca gtcgcctaga tacgaaacga ccattactcc
     5821 attattaaga ttttgaactg tagttattga actgacagca caatctcgac aactccaaat
     5881 cgctattaca aatatagata atattagaac aaaatttttc atcaaatttt atcaccttag
     5941 accatttact cgtacttgaa tcataatggg aaccgaacac gatacttact catcaatata
     6001 aatataattt tcagaattca attttttgag ccgatgaaat tcaacgtaat catttgaagt
     6061 cggtgtgcaa tactctttgt atattaacaa tggagatcgt ttgattgaga actcgacatc
     6121 attagcataa gtatttacaa cacacgtatc accattccat gaaataaaaa aaaccattac
     6181 tggattcatt acatcaagtt tattaaactt aggcataatg aatagatcgg gaattgtatc
     6241 cgcttcatat tcacatagag taatgtaata actacgatgg ctggttttaa tcattgttgc
     6301 agtatattta ccgtcttttg aacgaaaaaa aacacgctca cccccatctt tgcaagcaac
     6361 ggaactgata agaaataaaa gaatgatttt ctttaaatat ttaatacttg tgatacccat
     6421 attttgacag agttaaatca cctctgaatt ctcgctcaat ctatattcaa cttcgtgcat
     6481 cctcaccgca ttttagatat aaaacacaca tcactatcat aaatgagaat atgaacatat
     6541 atcgatattt tttttcttat caaaatcatc attttcaaac caatccatga taatagaact
     6601 acctttctat tgggactctc aaaacggggt ttattaaagt tgactggtca ttgaacaaaa
     6661 aaggatttct aaagcccatt gactactttc catgccaacg ttacataaaa tcaacgaatt
     6721 tttgtcaagt aacatagaaa cacagtcaat cattatgcca agcctcattg aatgggataa
     6781 aacttgtcag ttccaagaac gtaccttttt tcagttttca aacccttttc atcaagcttc
     6841 aaagcccgaa ggagtatttt gtttccggat tgagaaagct caaattcttg aacataagag
     6901 gtgttttcag gacatagttc agatttttcg ctatatgtca tgaaaagcac accatcaaat
     6961 actcttataa gaccaaagcg attatccaat ttaaaaacat gataaccttg catcatttca
     7021 cacataattg aataagggta acttgtaacc tcaaaaagca tactgctatt gatagaattc
     7081 gataaactcc atgtcttgac acttccaatt tcttctgccg acaacaacaa ataatctttt
     7141 gaagtacgtt ttagagctaa aattaaaccc gttctcagcc cagagatatc agctacaata
     7201 atcagcactg agtcttgctt cagtatatat cttattttag ataaatctat tgtatcgctc
     7261 atactaagat aattcttgta agacggtaaa acacgttttc caccaattaa gttaggtagt
     7321 ccatatccat atttaccaac aggtacaaaa aaagcaggaa gaagcccgtc aaatctagta
     7381 agtgtatcag caacgtcttg aatgctatct tctatagaag tggcggccaa cagcgagtca
     7441 ttaaacgcag tcggtacatt ttgtttggga gtttttgagc agccaatcaa aattaatatg
     7501 attaataata aggattcttt acacatatta ttttttataa gttctcatgt atattgggtc
     7561 aacaccagga ggagtagttt cagtctttcc gttaacccaa ccaggcacac tgaacggagg
     7621 ttccattcgc agtggactat agaagaacaa tgagccgttg gttcgattgt aatttccaaa
     7681 agcaatctta actgccgcag aaaccgaagc aaggaattgt tgaaattctc ttggatcatc
     7741 atttgttatt ttatatggat ttgtataatg atcataacgg ccgttacctt ttattgtaga
     7801 tgaaaactga acaccgtctt tcctatcttc taagacacct ttgtaagtgt ttccagtatg
     7861 cttgtatgtt tcactttctt ttctgttgtc tattatttcg gcagcggttt ctttttcaat
     7921 ttgagggcgc cctccaaatt cagcatatat ccctcttgca aaagccaaga ggtcatcatt
     7981 aataaaatac tccgtccaca tataatcgcc ccatttttga ccagcaattt caaaaaaaac
     8041 aggtacgcgt acaaaatatc taggtgggcc tggcgctggt actacagtaa cagatccatc
     8101 tatttcaaaa tagaatatgt caaaactccc gtcagggtca atttttatag tgggcatatt
     8161 tccgacaaat acgtatgggg tattatattc atcggcagga tccaccgcat gccacctacc
     8221 caattgcgga tcatacatac gccagccgta ttcgtaccag ttgagtccga agtcttcggt
     8281 tagttcttta ccattgtaga gatatttatt atcggacttc gagccaagtc cttcgatacg
     8341 catgccgaag gcgtagtagt ggctttccat cagtatatcc ggttgctgat tgggcgtcgg
     8401 cagcagatca gcaaatgaaa ttcgcgtatt gccaagatga tctttgatat cgtattcata
     8461 ctgtagatcg ccggttccgg ttcttttgat gcggccggtt tccgtaaaaa agtagtccaa
     8521 tgtataattc agattattcg atgtatatac gaatccgctg acatagtcaa tcgtggaagt
     8581 aactgttccg ttggtgtata ccgtttttct gagtttggct ccggttactg catatttcca
     8641 ctcaatcctg ttactgctgc ttccgaattc cactttggtg ggcaggttca ggtagttgta
     8701 ttcgatgctg gtgatgcctt tgtttttatc gagggtcatg ttaccattga tatcgtagca
     8761 tatgaatggt agtgcgtttt tttccgcact gtaaatgttc acgttatgga aggtgtgttt
     8821 tggaggctac aatcatcggc taataattat ttcttctgat tttttagcct tagctaactc
     8881 gattctttta cgcaaatctt cttcccgctc ttgccaatat ttcattttct catccgcgct
     8941 cattccctcc aatcgctgat gtataatttg ttgggctttg tgcttcatct ctacacaatc
     9001 aaaattcttc attttctatc acctccattg gtgaaaatat aataattggg ccatacccat
     9061 tgagaatatt tacggcattg tacatcggta ttttttcaaa atgcacgata tgtttaaaat
     9121 tccaactgat aatcatggaa cattctgaga cactagccaa agccacatga agagcatcct
     9181 cttctgattt tttcgaaagt atcccggcgt tgaggtatgc cttctgtaac tgtgtcgctt
     9241 ccggattgag cgtgagcata gaggcatagg ccagcatttc ctcaaaaaac atttttacat
     9301 tctcaggcgc cagcgaaatt tcatcggcta ccgtcggcga gatcacaagg gaaaaacgct
     9361 gttcttttat ttgttcaaag aaagttttgc tggctcgttc aaactccgga tcgaacatac
     9421 cgccaaacac cgatgtatcc acataaacac gattattttt cataccacct cgcatgctat
     9481 taaattaatg aatatctcgt atgattccaa tccaatattt cggcaagctc aatacaggta
     9541 tttcgacaaa cccaatacaa gttttgatac tcaccgatcg ggcgtatccg tcacggcgtg
     9601 gctgatcata tgagcgctct gttccgccgc gctcccgcac atacgcggga tacgaggcat
     9661 ctgaacggat aatctatagt gaagatatcc cccgaccggt cacgctgtca ggcgtatatc
     9721 agcgtccagt ggggcaaaga ggcaacgaag tagtctgccc cgcgctacga cgcggggtgt
     9781 agcaggactt aggcgaccgt cgggattcag aaggaaaaag gcggcgggag atcagccaga
     9841 ggctgaaagc gcgcggagat tcaaaaaaat ccggcagtcg gcaatgctca aaattgtatg
     9901 ctaaaataaa ttgtgatata ggtaattgca ttgcctatca aaagcgctgc gctggtctta
     9961 ccaatagact gtttccaaat taatcgaact acggttattt caattaatgc actaccggca
    10021 aacataacca caaacgacaa tatccaattc gctttactgt tcaaatcaga tagaaagtga
    10081 ggcccatacc ctgtcactgg tatcattgta aatgccatta ctgccgttcc aataactccc
    10141 gaacatatat taccaacaga tgacataata acagattgtt tttggcttat ccctatcatt
    10201 ttctttagga aaaaagcttc aataaaaatc gtcatcacaa ctacatacca aaaagcgaat
    10261 atccctttta atatgtatat tgctggccag accacattta ggacgctcaa aatcatttag
    10321 tcccccaatc tttccaagta tccgtattag gatcatacca ttgattggtt ctaaatggtc
    10381 cactttcaac aatcccctct ctatctctaa ctacaaaatt gtgtccaaca ataggagaat
    10441 aattgtcagg catatgaaga ttcagaactt gtgcactcat ttttttcaca aaatcctcaa
    10501 aaccttcttt gggccaaatg atacctaaaa acttctcaga tccagcagtt ttcatcctat
    10561 tttcactaag cgttgatcta caaaaaccat cacaattgtt atcctgcgta tcgtatttct
    10621 ttgtaacgtt ccttcttgca gatgctaaga gaagttgatc ttgttcgtgg gtcgtctgaa
    10681 catacgctcc tttttcgtag ccccagtaac cttctttttc aaaggcagcc aagttactaa
    10741 aatattgtcc atctgtgttg ctttctttac catccacaaa tccattttta gaaaacaaat
    10801 accaccccgt ctcgtcgttt ccaatcaata atgcagtatg tccactgaga cctgttccaa
    10861 atggcgcgtt ttgatcaagc aagtaaatta catcacgccc atctggatca acagttataa
    10921 ttggattgtt acttaacgct aagtatggtg aatgaaattc atcacttggg tcaaccacat
    10981 gccatctccc tatctgagga tcatagaacc gcgcaccata atgataccaa ttcagcccta
    11041 agtcatcttc aagctctttt ctattaaatg ttagtttgtt atccaatgca ttagatgaaa
    11101 ttccttcaat tctcatccca aatggatagt aatgagattc ctcctgaatt tccggttgac
    11161 cattattatc atcatcggta aaagttactc gcgtattgcc gacttgatcc gataacatat
    11221 actcgtaccg taatgatcca tttggcttac gcttaagtcg tccatgagtg ttagagaagt
    11281 attccaatac accattctta tacacgaacc ccgagacgta atcttgagtc aatgtcaaag
    11341 ctccacttgt atacacccgc ttctcaagct tcacccccga aaccgtataa gtccactcga
    11401 tgcgattctg atgatcggcc ccaaattcca cctttgtcgg cagattcaga taattgtatt
    11461 cgatgtttgt gatgcctttg tttttgtcct ctttcatgtt accattgaca tcgtagctgt
    11521 attcgtgtgt agaagcattg gatgcggaaa atgtaagttc attaccgtta tcggagaagt
    11581 cgtatttttg attattggcg atgtcggcga cggcgtcatt gaccgagtag aggcgattgc
    11641 cgatatagcc gtattggaga ttatccatgg taccgtaggt attggaagac cagttggttt
    11701 ttccggctct taggagcgac ttgatattgc cgttgcgatc ataggtcatc gtctcattgt
    11761 agatgcccgg ggtctgattc cacgtagcac cgctgtacga ttcgtactgg gcttcgctca
    11821 gttggttgag tttatcatag atatatccat aggcatgccg ggacgtaagg gatgtggacc
    11881 atatctgctg gctgatattg ccgttgtatt gcgcattacc tgcgatagcc gcatccggtg
    11941 attcgtactt cagttccata ccgaaaagat atgtccctag attagccggg tcgtttattt
    12001 tcgtcatcca gccgcggatg ttgtacttgt aattaaccgc ctgcaagaaa gtacttccgc
    12061 tattagtgga atgcaaatcc ttttcgatca gcgtgccgag ttcgttgtac acattcttgg
    12121 ccagcattac ttcgctgtca gtgttgtttt gttgatacgt tttgatgagt ctgccgccgt
    12181 cgtcgtagtc aaatcgtttt ttgaccgtga cggtggtgat agaggtcgtg tgtacggttt
    12241 tggtcttgag tactttaccg gggaagtcat actccgtatt tgaaacatcc gtcccaccta
    12301 aactgttatt actctgcgtt tggatgaccc ggccgtattt atcatagaag ctcacgctcg
    12361 aaagccaggt gacgccattg agcgctgccg cataagacga cggctgtttc acggcatctt
    12421 ttttggcctc cagcgtcatc tcgccggtga gcatgacctc gccctccagg ccgatctctt
    12481 tggcagagat attacccgaa ctctgcgcct gatttttgag cactaccgta tccggtgcac
    12541 tcgcaaatcc cgcataaccc tgtacgcctt ctaatatctt cgtcttcgct ccggtgacct
    12601 tgcccttcag gcggtcaaat ttgctgttat tgggaaactc gatatcgctc gcaaaggtat
    12661 agtcatcacc gcccgtatta tcaaaattat aatcatcata atacgtcacc gtccatacat
    12721 cgcagctcgc cagatcgggg aatgcctgat tggccgtata gccatgattg gtgctatagt
    12781 tcagtgatga gcgctcttca taaaatttct tacccgtcgc gctgccattt cccgcgatca
    12841 catacgtgtc caccagactt tgcatgtcaa cccgcgtcgt acatgtggta ttggtgtaga
    12901 gaccggtgag aatagtttag taaaatcaaa aaacgataca ggggagaagc ctctcccctc
    12961 gcctacggct ttcacgccaa ggcgtgacga tcctcggcgc ccttcccttt gaggggactg
    13021 cccacgcgtg gggcatcgga tatatgagtg tcaagcgcgt taattcctat cggaacttag
    13081 gcggcggctt gtcccctatg tgggattcag aagcaatgag gcggtgagag acgagagata
    13141 gcgaagagaa agatagctca gaacgtcaac tgatgattcg aacaccattg tgaccaagga
    13201 agaaaaaacg aattacgctg cacccaaaaa aactttaccc atttctagta ataaatcata
    13261 taccgtatga gcaattatag aaggtaccaa cgaccctgtt cgaatgtagt taattgaata
    13321 tacaatacca atgagaacac ttgttacaac agaatgcagg cccatcggcc aatgcataaa
    13381 accaaatagc agtgaagaaa taataacgaa agtgatcttg tttgcaaaaa aaagattcaa
    13441 aaaacctcta aaatagattt cttcaaaaaa acccgatcta atcgcgatca gaaaaccaac
    13501 aaggaaatca acaaagacac ttttttttgg tatttcgaca ggattcatcg ccaataaatt
    13561 tataggaatt aaaaacttaa ctatagaagt tgaaattgct tccacaagta ggatcaatat
    13621 aaaaaatgaa aaaacataat agtatttcgc cgaagacttt tttatgtttc caattgtagt
    13681 ggtaatacct atagatttcc aagttaatga tgtgtttttg attgcataaa tcaaaacgat
    13741 aaatggaatc actaataaag tgatttgaaa agcaaatatg tgttccggat gttcatgata
    13801 gaatttaggc ccgtaaaaat gatttatcaa aacatcaatg tttcccaaaa agaacaaata
    13861 caatatcttt ttgttaagtg acaaatgttt taccatgtta gccccgggct tgtcatctgg
    13921 ttacgaatcg tctctggtgt cacaggtttc acatagttct ttggggtgaa tgattcccga
    13981 ggaacgaagg ggtccttctt gacggttaat ggaactttaa gaccggatcg ttcgaggtat
    14041 ttctgaaaca aatcctccat tatcatcaaa ccaaggctgg gtttatattg aagtttaaca
    14101 gtataattga aaccactacc aaagtcttta ataatacttt tatcgttctt cattatgttt
    14161 acgccttgcc cgccaggact atgcactgtt gctccattgc ccaaacgcat tgaaaaattg
    14221 attgaaccgt tatcatttga ctgaacacgc aaaccgattg atttcataaa atcctcatta
    14281 tccgtttgac cagtttcaat caaatctttt aaagttactt tacctgctaa atctccttct
    14341 tttcctaagt gttcttctgc gatttgctga ggaataatta tacttggatt ccagttttta
    14401 acgcaacagg ttattgatta ataagttgat taaagacttc ataaggtttt tggaagttca
    14461 ggactttacg aggtcttccg tttaataggt tttggacttt cttgatttga tatctggata
    14521 cattgttaaa gtcggtgcct ttgggaaagt attgtcttac aagaccattg gtattttcat
    14581 tagtgccgcg ctcccaaggg cttcggggat gggcaaaata gactttcatg ccggtgatat
    14641 gggtaaacag cttatgctca gccatttcac ggccttgatc ataggtcagc gataatttca
    14701 tttgttgcgg taattttata acttctttag caaaggcttt ggcgagatca taggcatttc
    14761 ggtttttcag cggcactaag atcgttgttc gggttgtacg ttccaccaac gtccctaaag
    14821 cggagcgatt atatttaccc acgattaagt cgccctccca atggcctgga ataatgcgat
    14881 cttctagtga ttgagggcgt tcttcgatgc tgagcatatc ctctaaatta cgcacgggtt
    14941 ttgcaccccg ggtatggcga taccggcgtt tgcggttttg acgtaagcaa gagatgagtt
    15001 cttttttgag cgttcctcta ggcaagatat agatgtaggt atagatggtt tccgatgata
    15061 ggcgcatggt catttggcga aaggagaagt cttgagccag cattcggcta atttcttctc
    15121 gttccacttc gcttagtctt ttataatttt tcgtaagaat ctaactaatt attaaaatga
    15181 aattaaaagc aattcaccta ataaaaacct taatcatctt tgaaaacaca caaccatcaa
    15241 acacatttga atcatacaaa gaatggggga aagaatttgt gttaacaaaa taaataagac
    15301 gtaaccaata accaccattt gacaacgaat acctatttaa cggtatttga atgctgactt
    15361 gcaacgtatc tgacggcaac aaccttaccc tgcttgataa aatatcatcg tatgtgttcg
    15421 tactttcatg ctggcgtttc gaattgatgc tgtttgaacc aatagcttta ttgtttaaca
    15481 gtgtgtcaat atcaaaatga taaggcaaat ctcttacaag acacatctct tgagcagaat
    15541 cataaagtaa gacattaaaa ccattcaaaa cgacatctcg acaatcgtat ttgctttcca
    15601 ttaaaggccc gtttccaaaa acttcaggac gtggtattgt cttgttaaaa gccgtgtaaa
    15661 caattaatga gtcattagat ctatttatta aatagacaat agttgacaag gtatctgaat
    15721 ttgaattaac tattaactcg tcttgaatga agtttatggt taattttttt tcctcacatg
    15781 aagaaaaaac aatcaacaaa cacattaata aaaacggagt aaacctaatc gtattcttca
    15841 agagcttttt ggatatcatt gatatatcca tcccatttat gtgacgttgc ataattcaac
    15901 tcccaagcgt atgctgcttt ttcactcatg ttatgtgcat catctgaatt ttgagaatta
    15961 ccccacctaa catattcacc agaaaaataa tgtattgagt gtgtaagctc atgccctaat
    16021 gtcaaaaaga gtttctcagc agatgcaaat gctgatcggc tgattatatt gtatgttcta
    16081 aaacctattg acccatcaga agttttgaaa cccgaacgct gaattactcc tgtatatcct
    16141 gcactcattt gaccatcttt caaatcatac atactaccat caataacacc aatccttcta
    16201 ctaccaacag cttttaagtt ttcaggtgtc gttattctaa catcagagtt ccctacagct
    16261 ttatgaagtt tttgtaattc ttcactattt ttaattgctt tcgaggtttt cttggctaat
    16321 ttcaatagat tttttgggat cagaataacc tgctttaata gctctttgac gcgcaacatg
    16381 aagttgagat ccgtatgata gagatccacc gagagcacca attcaagcat taagtgctgt
    16441 attcagtcca aaatttgatt ttccgtcgga aatacgcgaa ctatataagc tacctgtcaa
    16501 acttgatgct attcctgatc gaattgaacc tcctaacgtt actccagctc cacctgcaaa
    16561 tcctgttata accccccccg gctctggaaa actttaaatc atggtaaaga aaataactct
    16621 ctacgcttag cctgcttaaa cactatttcc gggatgacaa cttcactacc attaattgat
    16681 agacccttaa tttcaaaatg taacttcttt ttggggtcag gcacgatagt tttgaatttt
    16741 atccaaaaac aactcgtgat tgaatcctct aacatgatag gctcgacagg tagtttccgt
    16801 tcaatcggtc tagtatcgct cgacgaacat tcgtaaggtc ctataaagcc atatggcaaa
    16861 tatttagtac tcgaatcagt ccaaaatata actttttgtg gatcaaagcg taaatcggta
    16921 caaaacggct tcaaagatat cccaactgaa aactctgagt ctatcggagt tgattcagaa
    16981 aacgggaatg gaattggaaa tataagaaaa cccgaatctg ctgcgttgag aggaaaaacg
    17041 cgataagcaa aatttttctc aacgatcatt tcaccaacat acggctcctc tactatttta
    17101 ttaaaattat tgttttccag atcaagttta ctgaagcgac tattggagat tcccacacat
    17161 cctgttatga aataaaaact tgcagcaata ctaaatagtt gcatcaattt gaatgtcttt
    17221 tgtttcattg aatttacctt tcaattgaat tccaattttg tgggtagaga atgaaaggaa
    17281 cacaaccaag tggtttataa atagatatat ccgaaaatta ttatttaaga actgagcatc
    17341 ctaatgtgca acctaaattc gatcatcttt aaagatggct cagaaaaaaa attcacacat
    17401 atacaaccgc ctatctacac tctatgcatt acaaacaaaa cttgtacaga agtgattatt
    17461 gactatttag atgataactc aagaagaaaa tcatgcttct ctttattcgt tttgctcaaa
    17521 aaatcgtcag cgattagtct atagaaagcc tctttgtgaa ttttattatt tttaaaatta
    17581 ttcgtatcat aacaagtgat acgtgggcca tgcacatata cgtttttttc tgcgtttact
    17641 gaatacttat attgactact atccgttatt ttatttacat gaattatcct atctttcgaa
    17701 taagaaattt cgaatgaagt ttttatagct atcgaatcat atgtttcaat aaaaccaatt
    17761 attgttcgcc cataaatttt tcctggaaac tcaaaaatgt aatggtttct gcccatggtt
    17821 gaataactat tcttctcttc atcacaactc agtattctca atagaacaat aacaattaat
    17881 attttcataa gctcaataat gattggttaa attatacgaa aataaatccg gtcttacacc
    17941 catagtatag gctttcgttg ttgcatacaa tatataagga tgtatcccaa tattaagtat
    18001 tccattaacc caaagagaca atgaagccat atttgcacaa ttaactgtgc ctccttggta
    18061 aggcaaaaat cttactaaac cccgaatagt tgcattcatt ggcatttgaa accattgaaa
    18121 tctgctatag ctggtaaaat attcattcta tgctttctta taaaaaactc aataaaccta
    18181 aagtgctcgc agtcatctta gtcgcaagca attgcactaa ggacccgtct tatttgaaac
    18241 acaaaacatc tatttatcat cgtcatcaaa aagatcagga tgtaaaggaa tagactttat
    18301 tagtctttcc ataagatgat ttttatgaca aaaaagattt tgcttttctc cacttttaat
    18361 gtttggttga attgttaaga ttgtagcttc ttctaaagga agtgttttcc cacaaaaaca
    18421 acaaattact ttatcactca agtttccatc catagttttt agctattctg ttaatagatt
    18481 ttagagaaaa attttccatt attgtaatta gtccgttatt gccttggaca acaacattaa
    18541 atctgccagg tgatacttca tgtagaaaga ctgttgcacc atctgcttga ctaaatgtcc
    18601 tacctaaagt tttgtagtat ttataccttc gaccgataat actccgcctc ttgtagcatt
    18661 agaccctgca atcctttttg agcatgatcc gttgtttgca tccccgcaac catatggtat
    18721 tccggatttc ctattgacgt tatttcaatc ggctttccag tccaagggtt tatcttgctc
    18781 tcttttgcgt aattatacct acagtacctc caatgattgc gccactgatt ccagtataaa
    18841 cataatttgc atccaaagct gcatctaaag ccgaaccaaa atcaccggtt ctgaaataac
    18901 ctcctgcgaa attacctacg aatcctcctg ccaatgatcc tacggcgcca gcaaaagcag
    18961 ttttaaaaac agggcttgtt atagaagtac cacctacatt tacagctaat gaagaagcaa
    19021 agtttcctgc tccgtagccg gcagcacttg caaaaccacc tacgatagcg ctttgagtga
    19081 tttggccagg attgtctccc cgctatgctg aatttgcacc tgaccccaac atacctccga
    19141 taatggcacc agttggccat ccaaagatac tcgccgtagc ctggccgcct cctacgagaa
    19201 aaaagctaaa tcctttacca aaattatcaa tcttatctcg attttggtag gcgttataaa
    19261 tcccactccc aacaattatc agtcccgcaa taagaggggc aatctttcca tcaggatcat
    19321 ttcgtaatgc tgggttgttt ccgacgtata aaaatggact actaaactgt cctgccggat
    19381 ccatcccata aaaccgcatc agatcgctat cacacatccg ggctttgtag ttatgcaacc
    19441 cgctcgtctt atcatattcc tgaccggtgt acatgtagtt tgtattgggc gttaccggcg
    19501 cgagaatact tccaaacgca ctgtagtcat atcccgtaac tgtcgtaccg tccactaccg
    19561 cacgtgtaga accgagatga tctttgatca cgtatttcca tgtggatgcg ctaccatagg
    19621 cgatgatgcc cgtaggtccg taaacgtatt cggtgactgt tcctgttttc atacgctcat
    19681 ggatcgcatt cataccatca tgcaaataca aagcttcact ggtcacaccg gataccacct
    19741 ctttgcggta tatgcgacgc tccgtaccat cgtattgaaa gttcatgcta ctcgcaccga
    19801 tagctatcgt gcgggtacga tggataaacg gatcgtaggt cagcgtaccg atccctttgg
    19861 gtgatgaaga agtgatattg ccggacgcac ataagtacgg ataccctcta tcggggtgat
    19921 gaataagcgc ttgatccgtg taccgacgcc cttttaacgc catgcgcaat gccgagagcg
    19981 ggccttcttt ggttaaatca gagcggaacg catagcccat aatcctctta gaataagcgt
    20041 ccgttatcag actcaagtat ccaaatcctt cgtcactagc cacataagta atatcgctca
    20101 cccatagctg ttcactacgg gttggtggcg gtagagcgct aattaaatta ggatattttc
    20161 gcacccaatg acgtgaatcc gtagtttgta cataccgccg cttgggtttg atcagcatac
    20221 ccgattcccg taacacggaa aataatttat cgcgtcccac gcgtaacccc ttttgctgaa
    20281 aggatgactt gagtaccata atacagcttg cgggtaccta tacgcggcat ttggctccgt
    20341 atctcttggc tcaggcggcg aaccgtttcc tgtacctctt ttgacctagt tccgcgcttg
    20401 gcttgcttat agaaagccga acggctgtaa cccaaaacac cggctattcg cccaacgccg
    20461 tatcaccggc tcccttgatc gccatagctt cggacagttg ggctaagtac ttttttcgta
    20521 tgtcgattcc cagctgcgcc tcagctatat ctatggcgcg ttgcaaaatc tccttatcct
    20581 cacgggcgcg ttccaaggct ttcaccaatt cccgaatccg tttaccgggg gttgctttct
    20641 tctgcatagt tgaactccaa tctaatcgcc cgtgtttaca aagccatacg tggagcgttg
    20701 attggacctg aatcccgtac ttcgacaacg cctcttttcg tgtaaaatcg cctttttcta
    20761 tcgcatcaac aacctgtaat ttgaacgcta aactgtaatc ccgttgcgtc cgcttcacat
    20821 acattctaca tccttgtcct tcttgcatct gctactcctc ctgtggacgt atttcaggac
    20881 gagacatttt gataaataaa aaaacccccg cacttcacag tacgggggtt tttgtttaca
    20941 aatcacaaag tactactttt tgcagcactc tttgggagtg catactttgg ttccgcagtt
    21001 ttctttatta gggcacgaac cgcaattatc ctgacccata cctgcaaaaa caaaagacgt
    21061 gaccatcaaa gcggccgcag cgacaagcat taattttttc atatataaat ttcctatttt
    21121 ttaaaaattg atatcatagc aaataacata ttcgccatat acaaaatact tttgtgatgt
    21181 ttatttttat gcgttcggga tgacagcgtt cggaggatgc caaatgatgc ggaggatttc
    21241 cggattatat ccggattccg gtgcggaaaa aatctgatgt tgtatctgtt ttcctggtga
    21301 aagggaaatt gattctactg attcataaat gcagcatacc tgacaactta caaccggagg
    21361 gcacgcttct ttagggcaat tttttttgga atcatgatgt tgcgatttgt tgcacgcacc
    21421 cgtacttgat ttaccgcagc aatttttctt ggtttgaaaa atcaacgacg atgtcatcgg
    21481 cacattagtc gccacaacaa acgatagtgt cagcaataaa acacttaaag aataaaaatc
    21541 gtgtgtactc ttaaatttca tacgtaacta aagattactc ttcaaatcgc ttaaaaacca
    21601 tgatccggat caggaaacgg agataaaact attgtaacta cgaaaaaaca agggtttggt
    21661 ttcaaaaaat cgttttttag tgcgttaaat accttgtttt ggtaatcttt ttttcatgac
    21721 tttacgccag atcggaggga taacgactaa ccaaaagcag ccgtaataac cggtgggaag
    21781 attaggactt tcccgatagg ttcttagttt ccaatattca cgggatgcga tatagtgatg
    21841 atcggcgtga cgtgtcaatt cgaccaaagt ccaacggctc caccgatgat tactttgcca
    21901 actatgctcg ggttgaaccc gcgaacccac aggcctttgc aatccccaat gccggatgta
    21961 gttcacatac tccagcaaaa aaacggcgaa ggcgctttga atcaaccagg caaacaaaat
    22021 aaatttcccc aataggaaat aaacgacgat ccatgaaact atttgtatta ctaaaaaaac
    22081 caaaaccgga ttgagccgcc atgaatttgc acgaggactc atgattttga aagcgtttat
    22141 aaattggccc ggaatcgtcc tgattaaaaa agaccaaaaa ttttcattct ctcgggctga
    22201 agccggatct tctaccgtag ccacgctttt gtgatgtccg cggacgtgtt caaaataaaa
    22261 atgcatatag ttaaccgtcc acaaaagaaa aacacccgta acgcgttgaa acgcgtatcg
    22321 gcgatgaatc aattcgtgcg cgatcacgat ggatgaaatg ccggagttca gaccggtaga
    22381 aatcgaagca agcacggcga aataattcca cccgtctcgt tggatcagaa gcgccaaact
    22441 aagcaaacaa acgatctgca aaaccgcatg cgtcatcaaa atggcatcaa aataaaacgg
    22501 atcggaatct tctaaattat tgctttccga ctcccccagc agccagtcta acaaaggata
    22561 gatcaacagt ccgactacag cgcctgacct cgcaaagtga ccgccaagcg tattacccgc
    22621 aacaacactc aatggataca gaaaactaaa aacgtatacc caccggcttt gagttttcat
    22681 aataataatc ttaacgcgtt ttttttagtg ctaatcagta cagaaacatg ggtttgtgca
    22741 tgacattaaa aactttcgga cgatagtttt ctacgtcata aagttctttt acatccacat
    22801 tgcgtttgcc ttgtatgatc gtttccgcgg gtaccgtagt atagcgccca tcctgcaaag
    22861 ccgtcatcat gccaaaacgg tttttggtca aaaggtcaat cgccaatccg gcaaaattca
    22921 tggccaccat acgatcaagc gcatccggtt caccgctgcg catgagatac gcgagagatt
    22981 ggtagatcat gtgctggcct gtgatttttt tgatagcctc cgccgtgatt tcgccgatgc
    23041 ctccgagttt tttatgcccg tatgcgtctt cctgaccgta ttcgatgatc ttgccgcctt
    23101 ccatggtagc gccttcggat atggtcatga tcgcataatt actgggattg ttccttttat
    23161 cgttcaaaag catttccgat agtttttcgg gatcaaacgg cacttcggaa ataatggccc
    23221 gatccgcatt ggccagcaga gctgaaatca acgacgtttc cccggagttg cggccaaata
    23281 actcaactac cgctatacgc tcgtgtgagc cggccggtgt tctgagcata ttgataaagt
    23341 gtaccgaacg cgtgacagcg gtagaaaatc cgatacagta atccgtacca tacacatcat
    23401 tatccatcgt tttgggaatc gctacgaccg gaaaatcttc gttatgcagg cgaacactat
    23461 aactcagcgt atcgtcgccg cctatcgtta ccaacgcgtc aatttttaaa tgcttgagca
    23521 cgcgaaaaat atgcggagta aaatcaattt ttgttttgga atccagtgta tcatgttcgg
    23581 gctttttcaa aaaatcgggc acatcgcttt tgttgacttt gccgggattg gttcgcgacg
    23641 tatgtaaaaa agtaccccca ctgcgatcaa tcgtgcgtgt attgataagg tccagcggtg
    23701 taatccattt tttactgcct tccggatcat caggattgta tctcagcaat ccaccccaac
    23761 cgcttcgtat tccgaccact tccataccgg catgaatcgc atcataaacc aacgctttga
    23821 tgcaaggatt gagacccggc acatcgccgc cacctgtcaa tactccgatc cgcttcataa
    23881 atacacctca ttaggttata aaatccatat aagatgagga ttcccgctac gaaatgacat
    23941 gagaaatgtt gggaaatata acttatgtgc tgtttaaata gaaatggaat taagtaaaag
    24001 tattctagga tagacgaaaa atcaggtgtt ttttttaccg tcaaaaaatt ccaattctgt
    24061 aggtttggtg tcgtcccatt cggatgcacg catgacgatt tgatcttttt ggcgaacgat
    24121 cgcgtatccg cgttgaagtg tatttttggg gttgagcgtt tgcagttggc gagctaacgc
    24181 ggtgatcgtc tgtccggaaa cgataagttt gtgacgcaaa acgcgcatca tagttttttc
    24241 aaaatcatcc acttgctggc ggtattgacc gactatgttt tccggttgac gaaaagcata
    24301 tctgttcttc agattatgca actgtttttg ataatcgctg attcgttttt ttacattttg
    24361 cgacagcgtg tattggagat tgacgatata acttcgcata tcttcctgat ctacggttac
    24421 catctccgcc gccgccgaag gcgtggccgc acgataatcg gcgacataat cactgatgga
    24481 gatgtctatt tcatggccga cagcgctgat cgtcggtatc gaacaggcat acatagcccg
    24541 cgccgtcacc tcttcattaa aagcccataa atcttccaat gacccgccgc ccctgcctat
    24601 gatcaaaaca tccacggctc cgtatttttc aaattcgtga attgcatgcg ctatttcctc
    24661 ggcggcccct tcgccttgta ctttgaccgg atacaaaatc aattcgatcc atggcattcg
    24721 tcgccgcgca acgctaagta tatcgcggat ggccgcaccg gtaggagaag ttatgatgcc
    24781 gacgcgctga atatattttg ggatagtttt tttatgcgag gtatcaaata atccttcagc
    24841 atagagcttt tgtttaagtt tttcgaaagc ctcctgcagc gctccaatgc cctcttgttg
    24901 gattttttga acggttattt gataataacc gcctttttca tataccgcta tgtttcctcg
    24961 agcaataact ttttttccgt ccttcaaagc atccgattgg gacgcggaag aacttttcca
    25021 tatcacagct ttgatctgtg cgccggcatc tttgagtgta aaatatacgt gaccactgga
    25081 ggcgcgggtc agattggaaa tttcgccgga aacggtaata gcagggaatg aatcttcgag
    25141 atgggttttt attaagcggg ttatttcggt aatggagtaa ataacaggct ttttctcctg
    25201 ccgttggccg agatcggatg aattctcatt tgaaactgaa aataaatcca ttctgattac
    25261 agcttcatta caaaattaag cccgccttgg aaaaaggcac cgtttatttt ggagtggacg
    25321 gtgcttttga atggagtagc cataataccg ccgccgatga acggggtgag atacatacct
    25381 tcgtctgttt ctagtctata acttacccgt ccaaatacgc taaaaaactt gacatccggt
    25441 cccggacgat aatacggttc gtccgtaata ttataaattt tcgcgtcgtg ctttttaaaa
    25501 aaatagtgcc cgccgacccc taatgtccaa gcaaaccctt gtttatattt tgacaaatag
    25561 ttattcatac aaatatgagc gccaaaacca tgtaccattt ccgttttatt agtcaacgag
    25621 gtatctaaaa cgatatatgg tgattcaaat tttactcgtc taaatgaata acccgcttct
    25681 aaactgtaag tgcttttatt ggatttgccc acagcctcca cgactacacc tgtaatcccg
    25741 gaataggatg tggtagcgcc aataagataa cttgttttgc ctttttcttt ttttgtttcc
    25801 ttttttggct ctactttttt ggtcggtttg cgtcgcacct gcgcatcggc tgataaccca
    25861 cacacagtaa aaagaacgat gaaaataaaa catccgatac gttttgctga actcattttt
    25921 tttccagatt tttaacttga aatcctttag gcgtttcttg tttaaagaca tccgagttga
    25981 tggaatggtt taatcggaaa tccgagaatt cccaaatcgt tttatcaccg tcggcttcac
    26041 taagttccgc ctgatagggt aaccacagat catcccttac gtaaataatg acgctttcca
    26101 ttttgtcata aaacttgctt ccgcgcttgg gaacaagtgt cagtttatat gaaccaaatt
    26161 ttttttgaac ttgtttggtg tccacttgta catcaaaata tttttcgagc atctcaagtg
    26221 aattaccaaa acctaataaa tactgactga aattaatttc attatcagac gtcgtcgtaa
    26281 cggttttatt ttttcgattg ataatccata tcgaattgtt gtttacgata tattggtttt
    26341 cggcttctga tgaaggattg agcgcatact tgcccggttt ttgataataa aaatccgctt
    26401 ttgtaacaat ctctttatcc ataaatgaag attgtttacg ttgtttcatt ttagcctgaa
    26461 gagaaattgt cgcttcattg gctgtccgca tgctggaaat aactaccttt ttttcttcat
    26521 cgccggatcc ggcatgtact gtgaatgaca aagaaaaaag aacggtaata caaagactga
    26581 ttattcgttt ttctgatttt ctcataaata tctttcttga tctgattttc cgttgatgaa
    26641 ttccggtgaa cctacggaat ccatatccgc tttttgataa acgtaaacgc atacaaacat
    26701 agtatgataa aaattttgat tcatgcaatc aatcataatc aacacatcta aaacacatcc
    26761 gtctaacaac aatgattaca tcgggatcag gacgacataa atcaacagga aatgtttttg
    26821 caaattacgg tatctactcc tacatttcga ggattgtatt tagaataata agaaaaaatt
    26881 catggcatta cgaaaactgg cgtatgatga aataaagcag ctccgctcaa cacccgaaga
    26941 attagtttac aaaacgcgat ttcctatata cggtgttttg gaaaacatac gtagtatgta
    27001 caatgtcgga gcggcatttc gtacatcgga cgccgcgtgt attcaagagc ttattctctg
    27061 cggttatacg gcacaacctc cgcgctcgca aatcgaaaaa acagcgctcg gcgctacaga
    27121 aactgtccca tggcgacact ttaataacac tcttgatagc attaattatc taaaagatcg
    27181 aggcgttaca gtcgttgctt tggagcattg cgaagaaagt aaaaatttat taactacgga
    27241 gttccctttc ccggtcgcgt tatttgtggg caacgaagtg gatggtttaa acgaagaaac
    27301 cgtcgctcat tgcgatatgg cttgtgaaat accgatgttt ggctctaaac aatccctcaa
    27361 tgcggccgtt gcttatggta tcgctgtgtt tgaattgacc aggaaatact tagttttgca
    27421 agagaaaggg taatgctatg cgatcattga tggttctttt tttatttatt aatttgacaa
    27481 tttacggtca ggagttttcg atcggttgga ctaaagctag tacagagagc ggatctgagt
    27541 ttaaaaaata tgaaccccaa accgcgtgtt tttttataaa acaccggcgc ctttacaacg
    27601 aggattttat gtggggttta ggattagata tgatgtcttt tcgtaagtcc gcatctccag
    27661 aacgcacgat aaagataaaa ggaattaccg gtgaaatagg ttattatttc cgacaaatac
    27721 ccggtttgac cgtcttaggt tcaacaagct ttgggctaat gcgatgcgag tacgacttca
    27781 ttgatcctcc agcagatttg aaaggctctt caggaaccgg tgtttctata gaaccctccg
    27841 tatcaatttt tttcccctta agtaaaactc ttattttttc accgtccatc ggttatcata
    27901 tgctctttga taagatgaat tttaaaacga caaaccctat aaaacaacct attggaaaac
    27961 tcgattctca catgttcgct atgacctatc gccttgcctt gtcttggcgc tttcggaaag
    28021 aataacacaa tgatttttaa aaaaaacaca tactctaatg atgaaataaa catacaacga
    28081 atcagcccgg atattgattt gtttaaggaa ataaatcgac tgcgcaaaga tttgaatgcc
    28141 gttatccttg ctcactatta tcaggaatcg gaaattcagg atttggcaga ctttgtaggt
    28201 gatagtttag aattagcaag gcaagccgcc aaaacgaaag cggatgtgat cgtttttgcc
    28261 ggtgtgcatt ttatggccga gaccgctaaa atactcaacc cggaaaaatt agttttacta
    28321 cccgatctta aggccggatg ttctttagcc gacagttgcc ccactgatga attcaaaaaa
    28381 tttcgtgaag cgcatcccga tcattttgcc attacttaca tcaattgtac ggccgaagtc
    28441 aaagcattaa gcgatctgat ttgtacatca agcaatgccg aacggcttat cgctcagatc
    28501 cccgaatcta cacccatctt gttttcaccg gataaaaacc tcggcgctta tatacgtaag
    28561 aaaaccggtc gccccatgat tttgtggcaa ggctcatgta tggtacatga gattttttct
    28621 gaaaaaaaat tagtacaact gcgggcaaca caccccgaag ctaaaatcat cgcacatcct
    28681 gaatgtgaag agaccgtact caatatggcg gactttatcg gatccacaag cgggcttctc
    28741 aaatttgtcc aacaatcgcc ggatcataca tttattgtcg cgacggaggc cggcattatc
    28801 catcaaatgg aaaaagcatg cccggacaaa acatttatcg cagcgcctcc caataataac
    28861 tgcgcttgca atgaatgccc gcatatgaaa ctgaatactt tagaaaaact atatctctgc
    28921 atgaaaaaca aaactccgga aattacactt ccggaggatg tacgcacacg cgcattaaag
    28981 ccgattttgc gaatgcttga aatgagttaa cttgttccaa taactcattg tcataaacgt
    29041 ttatttttta ctcagtctct tttttaaaac attaatattc ttttgtataa catccgcatc
    29101 gcgttgcgca ggatcaagcg caagtgtttg ctctagatga tgtatcgctt ttttgatctg
    29161 ctttttatcg ttcatatata cgatccctaa actgcgatgg atatttgcgt tgtttggatt
    29221 caaagccagt gatttttcaa acaacgattg cgcttcttta tgttgttttt ttcgtttaag
    29281 cagaataccc aaattgagat atgcatcgta taaattagaa tccaaaacaa tcgctttacg
    29341 ataatgtatt tcggctttga cgtaatccaa gttgtcatca tatccgacag cacatccgta
    29401 gtgtactaag gcacggagcg aatctatttt taacgctgat tcataagcca ccaacgcttc
    29461 gggtgcgttt cctttttcgc ccaacactac acctaagtta acccacgcat ctacatattt
    29521 tgaattgagt tccaaggctc tgcgatattg taatacggca acatcccact cttttcgcgc
    29581 ctgataaaca tttcccaaat taaaaacagc gtcggcattt tcgggttgaa attcgatagc
    29641 acgattatag ccggtgatag ccgaaggcca atggctgtta cgataggcca tattagccgc
    29701 tcgaagatat accgccgata tatttttttc cgattttagc aaattattat aatccaaacg
    29761 acgggctttt tcgaaaaaca aaatcgcatc ggaatcgtta tttttttcaa aagccgaaac
    29821 gcctaaactg taataaccaa gagcctccca tttttgaaca taaagcgcat actcttcgtg
    29881 atcactcgcg atcgtccgca atctttcatg ccccagcaac acaccgtttg gactcaaaat
    29941 atttttttct gtaaggttga ttcgttggat taagttttca accgcacggt acatggtttt
    30001 atagaaatcc gtgtagtttt taattggtcc atagattcgc tcttttgcga tgtttacatt
    30061 accgttatcg cccaaaagtt caaactccat ctcgatggag tcgcttcgca tacaataatt
    30121 tcctcgcaca atgaagtctg ttttatcatc aactatgaga tctaatttct ctgaatgttt
    30181 atcacccgat ataacgcttt tgttttcaaa cctattaaat attgtatctt gtgaaattat
    30241 tgtcaattct ctagtggcgc tcaacacatc cgaaagcata tagcttagat aataagttgt
    30301 tttattatcc gagctacaag tcgatgtaaa cggaagtact gcaatttttt tattttgtgc
    30361 tgtgagagta tatgcgcata taaaaagtat cgtagcaaaa acaaaccgtt tcatgcctta
    30421 agacctccat ttgcgaccgt ctttgttcaa taacttatcc gccgcttccg gcccccaaga
    30481 acctgcaaca taattcggaa actccgcttt attgtttttc caatgttcca aaaccggcat
    30541 tacgagccac caagctacct cgaccatatc tttacgcata aaaagcgttg cgtcgccgcg
    30601 catacagtca agtatcaatc tttgataggc ttcggcaatt tccacctgaa atgcttcttt
    30661 ataattaaaa tgcatgtcta ccgactggag ctgcatttcc ggacccggta ctttggcttc
    30721 aaagctcatc gtaatacttt catccggctg gatttgtatc accaatacat tgggcgccac
    30781 atcatcggcc gccgtctggg taaacatcat atgcggtgtc cgtttaaaaa agatcgctat
    30841 ctccgaatca cgacgaggca ttctttttcc agtccgcaaa taaaatggta cgcccgccca
    30901 gcgccaatta tccacataaa attttgctgc gacataggtt tcggtatttg attgcggatc
    30961 cactttcggc tcggtacgat agccgggaac cggtttgcca ttgatctgcc cggctccgta
    31021 ttgaccgcga acaaaataat cattcacctg atctggtttt acgggacgaa tggcgcgcat
    31081 gactttgacg cgctcgtcac gtacagcatt ggcatcaaat gtagtaggcg gttccatcgc
    31141 cacgagagcc atgacctgaa gcacatgatt ttgtatcata tctctcaaag cgccggcatt
    31201 gtcataataa ccgccccgat cttcgacccc caaagcttcc gccgctgtta tttgaatatg
    31261 gtctatataa ttgcgattcc acaaaggttc aaaaatcccg ttcgcaaaac gaaacgccaa
    31321 tacgttttga acggtttctt ttccgagata gtgatctata cgatagacct gccattcttc
    31381 gaaagcttta gccacttccg cattgagtgc acgcgctgac tcaaggtcac gaccaaatgg
    31441 tttttcaata ataatgcgcc gccagtaatt acctccacgt acttgcgaca tattgtgctc
    31501 agccaatttc tgacatatcg tagcgaatac tgtaggcggc gtcgcgagat aatagatacg
    31561 gttatcatta caggcacgcg tcgtacagtt gtttttgatc agcgaatcca tagtttgata
    31621 taacgcattc tcatcaaaac ctccgccgac ataaaaggag ttttgaacga attcattcac
    31681 gattttcgga tctacggcgt cggatttggc aaattcacgc aacgccgaac ttaccatttc
    31741 acggtatgct tcgctactca tctccggttt gccgcatcct atcactgtga atttctcagg
    31801 cagcaggcct tctttggcgc tattaaaaag cgccggtaaa attttgcgcc gcgccagatc
    31861 accggaaccg ccgaagataa tcaccgaaca tgtaccggca cgcgcatcgg atttgacctg
    31921 tcgcaaagct tctgtgacgt gagctgccat gaagagcctc cagataaaag ttatacagtt
    31981 atttcaaatc aatatgataa caaacaatca tccgttttta agcacaacga agtgtgatca
    32041 cggtgatttc gggtggagaa ccgatacgca ttggcggacc ccaataaccg gttccgcgat
    32101 tgacatacac ccacatatta tcatgccggt gcagtccttt caaatacggt tgcgccatcg
    32161 ccgccaatag attacccgga aaaaattgtc cgccgtgtgt gtgtcctgac aactgcaaat
    32221 caaaacccgc tgccgatgct gccggcgcac ttttaggttg atgcgcaagc aatattcgcg
    32281 aataatcgga tgatgtgcca tcaatagctt ttacaggatc ggataaatgc ccgggaatga
    32341 aatccgttgc cccataatcc gttataccgg cgagacaaat ctgctgattt tcacgatgaa
    32401 taagacggtg ttcattgagc aatacatcaa atcctaatct tttagcttca tcacaccaag
    32461 cttttgcccc ggagtaatac tcatgattac ctgtgacaaa aaaaacacca tgcggtgctt
    32521 gaagttgctt cagcggttcc acttcctcac gaagccaagc taccgtaccg tcaaccaaat
    32581 cgccggtgaa cgccactaag tcaggtttta gcgccattac atgatcagta acacgctgga
    32641 caaattcgcg tttaatagtc ggtcccacat gaagatctga aaactgtacg atccgaaatc
    32701 catcgaaatt tttaggtaga ttttgaagcg gtacttcaat cgcttcgact accgggtcat
    32761 tgtgcgcctt aaaaatacca taccctgcaa ccgccgtaca aaagccggcc acaccgagat
    32821 ttacactttg aatgagaaac tgtcttcgag tttcgtctgt aacggtttca ttgggttttg
    32881 tgaacagcca tgtaaaacca tggtgcaacc atccacctgc atcccgaagt aaaaatgcaa
    32941 ctaataggag actgaacaat ccaagactcg aatatccgca ccacagtaga acatccaaac
    33001 ttatgctttc gtgctgacgc tgcaatacca tcataaatcc gccaagcggt aaaagacata
    33061 aaacagcgat cactatccat ccgatataga ccggtaaacc aaaatgagcc atagccggta
    33121 cgatgcggcg acctacatac caataacctg acataatgat ggccgtccac gtcagaaaaa
    33181 accagataaa tcgcattaaa ttaattagta tccgataatt ttgattctaa aaaataaggt
    33241 cggatggatc atccgacctt ttataacttt cagaaattct attcgtttac tgtttgatcc
    33301 aggttttttc aaaacgacgc gtagcactgg catgaagctc aaatcccatg acattatccc
    33361 gcattggaac gatcgttaac gcatgagcta aagtaaccca tggaaaatct ttattaaaaa
    33421 cttcgcacgc ttttttgtat atatccgctc gtgcattggg atcaaatgtt tgacgaccgc
    33481 gttttataag attgtgcatt tcttcagaag cataaaacga tacattggta gacgattgtt
    33541 gtttggtaat atctttatcc aaaagcggat aaaagaaaaa gtgcggatcc ggagcatcac
    33601 cgacccatcc ggaaacagac atctcatgtt ccccgcgccc gcgccgacgt aaaaattcgc
    33661 cccagtttga tattttttct tcttccgcgg ttatacctac agctttaaga tcgttgatga
    33721 tcagttccgc agctaaaggc gggttgggca tataatcgcg aaccagcggc aaaccccata
    33781 gtttgcattt gaatccgttt ggaaaaccgg cttctttgag cagttgtttg gcgcgttccg
    33841 gatcataagg tgctgcctgt atctgatcat tgtgacctaa aagagtagga ggaaccgggt
    33901 ttttggcctg gctaccgagt tgtgcaaata cttcgcgtac aatccgttct ttgttgatcg
    33961 ccaatacgat tgcctctcgc actttgggat tatcaaatgg cttttttctc atattcatac
    34021 ctacataagc cacattcata ccgggctgtt tggttacttt tactccggga gttttttcga
    34081 tatccgcgag atccttttga gtcggcaaac ccatcatatt gatcaccccc gatttcaatg
    34141 caagccaacg ttcatgcgga tcaggtacag gtctgaagat caccgtatca agatagggac
    34201 gcccgtgcca atacccatca aaagcggctg tacgcactga gccgtccgta tcccagctga
    34261 caaatttgaa aggaccggcg ccgacgggat ttttgctaaa ttctgcgccg tatttttgta
    34321 cggccgcagg acttactatg gctaaaaaat tgagcgacaa gatattgaga aacgtggcat
    34381 cggctttttg taaaacgatt tttaccgtag aatcgtttat ggcttcgatg gattgaaaaa
    34441 tattgtccat attaaaattt ttccaaagcg catagccact gctgctttcc ccttcgccgc
    34501 ccgatttttg gcgttcaaat gtaaaaatta cagattgcgc gttgaacggc gtaccgtcat
    34561 gaaaagtcaa tcctgttttt aggttgaatg tataagtacg gccatccaaa gaaatcttcc
    34621 atgatttggc aagtcccgga tccaatgcaa ttttgccggc acgttgaatg atcaaacctt
    34681 caaaaagatt tcctactata atagaagatt ccgtttcctc cacaaacgcc ggatcaagtg
    34741 ttaccggggg accgttctta ccatagatca ctgtaccgcc atattgcggt gtgtctccgg
    34801 ttgcatcact tacagcatcg ttttttttac aggccgttgt aaatacaagt gccaccgcta
    34861 aaacgatctg aaataaagat cgttgattca taaatacatc ctttcggtat gaatgatgta
    34921 gtatggaaat agttttccaa tcacgctatg ttaggatttg acccacacat tcttcagatt
    34981 acgtgtataa ctagcatacg ggttaaaatt cattacttca cgccgacgcg gaatcacaat
    35041 ttgcgtatgt gcgatggtaa accaaggtac atcacgttgg aatatttgac acgcgttttt
    35101 atatagctga tcacgctctt ccgtttttgt agctgcttta gctttaagca aaacgcgatg
    35161 catttcgtcg ttatcataaa aagcgctgtt tccggttcct ttttgacgca cggtggtttt
    35221 ggataagaga ggataaaaga aattatcggg atctggaata tcggcaatcc agcctccgat
    35281 caacaaatcg taatcaccga gataggtatc ctgaatatat gtctcccacg gcggagtaac
    35341 tacctcgatc tcaataccta ctttttccaa ttcactacga atcatttcaa cggctttttc
    35401 cgggtgcggc atgtattctc tgggcaaagg cacattccac aatttcagtt tgaggtttct
    35461 tttatatccg gcttctttca gaagcctttc tgattctgcc ggattatatg gtgttggacg
    35521 aagatcgtca ttataaccga gcatacccgg aggtatgggg ttttttgccg gcacaccaaa
    35581 gctgccataa acggcttgaa ccagtttttc acgattgatc gcataagcta cggcttttcg
    35641 cacacgaatg tctgacatgg gttttttgct catgttcatt gccaaaaaag agacattagc
    35701 gccgggttgt ttaaattgaa taatttgctc attgcgtttg actcgagcca acatgtcaaa
    35761 cggcggcggt tcgatcaaat gaaattcgcc ttgttccaaa cgcgtaattc gtttttccgg
    35821 gtcgggctca ctcaaaaatc gtatggtgtc caaataagga cgctcacccc aataattggg
    35881 attggctaca agcgaaagat cattccaatc gctggaagca ctcatcatga aagcgcctgt
    35941 accgacggga tgtttttcaa aatccgcgcc gtatttttcc acagccgtag gacttactat
    36001 agccaaaaac tgcatactca gaattttcaa aaaagtagca tccgcacggt gaagtttgaa
    36061 ttgtaccgtt gaatcattga cggcgcggac ttctcgaatg agttcatcca tattaaaatt
    36121 gatccagtta gaaaactggt cagcggagcg acgggcgccc tttttttggc gatccaaact
    36181 atataaaact gctttggcat caaacggtgt cccgtcgtga aaacgtacgc cgctttgaag
    36241 attaaacgta tagcttaatc cgtctacggc gatgatccac gatttggcca gccctggcac
    36301 aacatccaca ttacccggtt ggaggcgcac taaggtttca aaaatattat cggtcagaat
    36361 agaggattcc gtctccgcaa ctttggccgg atctagtgtg accggcggac catgtttacc
    36421 gtagagtaat gtacctccaa agcgaggttg attatcggaa acagtattta cgttttcttt
    36481 tttggaacat ccgctcacaa aaaagaacat aaacgataat atcagtaaca aaaaacggaa
    36541 ggggatttta taattcggca tgttgtaagt aattatgata tcggttgaag ttgaagttca
    36601 aaaggcatga gttgagcagc gctatccatc ttgatgcggg cgtattcgat gatttcctga
    36661 ttttcgagcg actgctcttt tagaccgtct gctgcgatga catacttatt cgtcgctaaa
    36721 gcttctttga taaatgtcat tgaactgaat gtttcagaca aaatactgct gagcgttatc
    36781 aaataaaact gacggtacaa ttcggatttg ccgagcgctt cgatttcatt tcgtgcactt
    36841 ttgaggtaat caggaaattt cccgggttcg atcgtatacg ttttttccgc ttcttctccg
    36901 gtttccggat cgatcatcgg ttttccgttt ttatcttttt tgatcacctt gacatcaatt
    36961 tttccatacg taccggcaaa agccttttgt aagatatcat gaagcggcaa tggttcacgt
    37021 tgtgccgcta caccgagatc ggcacgcagc ctttcgacaa gctgccgcac acccaactca
    37081 acgcgcaact cattggagcg cagtttggtc gacgaataac ttagtaaagc cacggcttca
    37141 tcggacagtc cgagcgcttt ttgataaaac gccgtttcat cttttatggt acgattgaca
    37201 aaaccaagca gcagctctcg ggcgtctact tgtttacgca gggtggacac gtctatgttt
    37261 tcacgcgctt ctttgtattc gtattgttca aggatacgat tgggatcaat attttttata
    37321 ccgcgtaaaa gttccgtctg tgtcattttg atcagcggac gtttaccttt acggcgcaat
    37381 tcgtgcacct ccatatccca accggatgca acaaactggg caacgagaat actccgcact
    37441 ttttcatact taccataata gccagcggca aactttcctt cgctttccag ttctcgtttt
    37501 tcacgatctt cttcgttaga aacaacgtcg agaaacttgt catcaatgat cttataaact
    37561 ttctgtcgca attcggtgcg cgcaacatca atctcttctt gcgtatgttc ccttttgtag
    37621 atgtagtcaa agagaatctg atcatccaaa aggcggagcg cttgagctaa ttcgcgatta
    37681 ttgagatagt gatacaaaat gatccattgt tcttcaaacg gacgactttt aagcggtgtt
    37741 tgtttttcct tttcgggcac aactgcgttt tgtttatcaa atccgttata atacaatgtc
    37801 tcacgacccg tatgtgaacc gtcaaattca taccgaagtt ttttgatttc ctgaaacaat
    37861 gtcagacggc gcgtaaagct gtaggtcaaa gaaagacggc ctgcaagtac tttgatcaat
    37921 tgatccgaaa cgatgtcgcc cagttcgtgc accaaaagtg cggccacata aaacgacatg
    37981 agcgcggagg cgtgacgttt ttcagcggca cgctcgataa acatttcgtg aataagaggg
    38041 aagtccgtct gatccgtaag atacgcccgg aaaagatcgt gaacacgttc aaacccttcc
    38101 aaggacgccg tattgctcat catacgatta aaaacaaagc gcacttcaag atcaggcacg
    38161 cgcgcgcgat attgcgcagg aagattttgc atcaccttgt cgctgttgat cgtctcaata
    38221 tcaagcttga tttcccggca aaacatttca acaaccgctt catccacttc gacgttattt
    38281 ttgacggcgc caagcagttt cccgaacgcg tacatt
//
LOCUS       DAOQNQ010000004        38167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_344_length_38167_cov_6.145466, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000004 DAOQNQ010000000
VERSION     DAOQNQ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 38167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38167
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_344_length_38167_cov_6.145466"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PK754_00575"
     CDS             <1..601
                     /locus_tag="PK754_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNB08028.1"
                     /translation="STALAGASRDFSESSSNILFAFILALVLIYLVLAAQFESFVDPF
                     IIMLTVPMALAGAILSLWWFQQTLNIFSQIGIIMLIGLVTKNGILIVEFANQLREKGK
                     NKMDSILEASVLRFRPILMTSLATVLGAVPIALALGAGSISRIAMGIVIIGGVIFSLI
                     LTLYVIPAIYSYMKHEHKPGQETNIAPLQQTESLSKEHV"
     gene            complement(677..859)
                     /locus_tag="PK754_00580"
     CDS             complement(677..859)
                     /locus_tag="PK754_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNB08029.1"
                     /translation="MLEKTIRVIAGTFVLISLALGYWVHPNWLFFTAFVGINLIQSSF
                     TGWCLMETILKKTVFR"
     gene            complement(869..1174)
                     /locus_tag="PK754_00585"
     CDS             complement(869..1174)
                     /locus_tag="PK754_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08030.1"
                     /translation="MKLIAVMTIEQYAQDLRKLFREHHVIIFSESEIRGYRSDATERD
                     SSNWFAHTASPIYSHLVFAFVDESKAAELMQAIRNHGQTMDLDHPMRAFQMHVEQFA"
     gene            complement(1176..4424)
                     /locus_tag="PK754_00590"
     CDS             complement(1176..4424)
                     /locus_tag="PK754_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNB08031.1"
                     /translation="MKTGFSGRIAQAFINSKLTPLLIAGFLAIGLFATYLTPSEEEPQ
                     IVVPMADVFVQYPGAEPKEIETKVIQPLEKIISNIRGVEYVYSTSMQDQGMLIVRFVV
                     GEDAERAIIRLYDEVFKNMDKMPAGVRPPFIKSKSVDDVPIFAMTLWSARYDALDLRR
                     FAAELSSEYKKINDVSEVKIIGGRSRQVRVILDKEKMAAYRLDPLTIIPFFQSAHQQG
                     PAGKFNQGNTEYLVQTGKFLETADDVRQLVVGMHLGKPVYLKNIAQIEDGPDEIKDYV
                     TFGYGAAHAGEHKALEYPAVTLSVSKRKGSDAMGLGERLMEKSEALKGKLIPSDVTMT
                     VTRNYGETASEKVHELLKHLSAAILAVTVLVALTMGWRSGLVIFMTVPVTFAMTLFVY
                     YMFGYTLNRITLFALVFVTGLVVDDAIIVVENMHRHFKMRKLPFLQAAIASIDEVGNP
                     TILATFTVIASVLPMAFVSGLMGPYMSPMPIGASLAMLFSLFVALIITPYLAYRLLRG
                     DTHGHSEKEYRLETTGIYKLYTRIFVPLLENAKKRWWFIGSVVVLLLASMSLMYFKLV
                     TVKMLPFDNKNELQVVIDMPEGATLEKTAALSKEIAAYLRNVPEVTHYQMYTGTSAPI
                     NFNGLVRHYDLRQGSNVADIQVNFLPKGERSEQSHDIAKRIRPAIVKIGASYGARIKI
                     AEVPPGPPVLSTLVAEVYGPDYTKQIEVAHQVRTAFETSPGVVDVDWLVEDDQTEYRF
                     EIDKEKAAQSGVSPQQIYHVLGIALNGKQIGALYQAKELETVGIQLQLAESQRSSVDD
                     LKNIHVIAMTGIPVAVSDLVRIYPQTREKSIYRKNQQRVVYVTGDVAGEVESPVYAIL
                     NIDEAMKKIRLPEGYTLQTLYAKQPFLEENFSMKWDGEWQITYEVFRDLGAAFAAVIV
                     IIYFLIIGWFQSFKVPFVMMVAIPLSLVGILVGHWMMDAFFTATSMIGMIALAGVMVR
                     NSVLLIDFIELRLQDGVPLKTAIIESGAVRTTPILLTSGAVVIGAFVILFDPIFQGLA
                     ISLIGGAIASTALTLIMVPLFYYMTEKAQYESEAVPQASVPLETTENESKEN"
     gene            complement(4421..5503)
                     /locus_tag="PK754_00595"
     CDS             complement(4421..5503)
                     /locus_tag="PK754_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB08032.1"
                     /translation="MQKCFIGLSLSVMMIVMVACGSPEKNQPNQTATPVRTITIRQKP
                     VNEKTALAGTLQGDKRITLSTKIMGQVLAIHAHIGDRVAKGQLLIKIKSDDLSAKRAQ
                     IAANKIEAEAALKNMESNYHRIKELYARKSATQKEMDDTEMAYAMTQAKLKAVEEMEK
                     EINDVFGYSDITAPIDGYVVQKLTEVGNTAAPGMPLMVIEDLSSFKVIIPIPESDAAS
                     IRIQDAVEAEFSSDPKHRLNGYVSALSPSAVPGSRQYDAEVKLHGITENLKKTLRSGM
                     FVRIYAGAGQKNMITVPDSVIHRRGQLEGVFVVSAANQALLRWIRPGKSYGNEVEVLS
                     GLDFGDRVIYESTERLSDGDRVEVRP"
     gene            complement(5521..6846)
                     /locus_tag="PK754_00600"
     CDS             complement(5521..6846)
                     /locus_tag="PK754_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNB08033.1"
                     /translation="MKSLCFGVFLLFVLVGQVKAGQDGTRYTLDSVLQTASRQNYDLA
                     SVQAEMDAMRADYRKSLSLFLPQLSVSEIYTRTNDPLNVFGLKLKQEIVTQADFNPVT
                     LNQPKAVDNYTTKLEWRQPLLNMDGFFGFRAASSGVKAMEEKSKRTSYYITYQAKIAY
                     YQLLVALQSREVIRKALTAAQTGSEQARNFYDQGLIRRSDVLFAELRLKELEAKQLET
                     ENMIDNANNQLKLLMGLSGDEVIIPTDTLGGPLALEMTTPHDMTNRSDIRAMRYQMSA
                     QRAMQSMAWSKFLPSINGFATYEYNDASLFGRQAKNWMVGAMLRWDIFSGFDQISAIQ
                     KSNAQYRQTETGYKKALTQSQYDLNAALRQWKVSTRQVVIAEQAVQQSEENYRVLSDR
                     YHQGLDRTTDLLMAEAALSQARLQLIHARFAWHAAMFTVELLTEQSIKN"
     gene            7005..8561
                     /locus_tag="PK754_00605"
     CDS             7005..8561
                     /locus_tag="PK754_00605"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNB08034.1"
                     /translation="MYYLLIALVLLTKAVLAGDAPDWVIQPGKSAAFPASAYIVGYGS
                     ASAQNPDAQKIAEDNARAALSGSIVTDLRSTVISRLEEQGKNSAEYFSSVTQSSTGLQ
                     LLGISIETYKDKKLVHVLAYAKRTELSATYNKKREALISNIAAIITQAKAAEESKKIE
                     DAAALYLSLFPMYEELREAEIVLTVVHRESQVSQPDKTIPSRTEIAQTIDRLLAQSIT
                     SVEDAARAVAYQVSKQISGTTGSFLVSPMTYQDSKMSSPFARYFQQALETQFQKYAVW
                     EVARQARGFQPKSSQITRDLVIASGAGCIFEGNYWEQGDRIKLIGRLRDVQNGRIMAA
                     VEVTLDIAHIQKTGLSIKPENMLKALADQKAFAEEEVISSDIQLDVWTNKGTENLLFA
                     EGEIMKIYVRTNRAAHIRILYNFADGSRTLLYDDHYIDEAKANQVVEIPGEFECTGPF
                     GAEMMVVVASVGKLPEVKTYSVDGIAYLDEKDAKKAAYGTRGFKKKDPASSTSPQQTE
                     KKLTITTIAK"
     gene            complement(8596..9645)
                     /locus_tag="PK754_00610"
     CDS             complement(8596..9645)
                     /locus_tag="PK754_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNB08035.1"
                     /translation="MKFDDLMKIFNQLLLLTDGDVSKALSYMTQIDRQYHIFNEDMDF
                     QKFYDRLVQEGYIEESQDGSAPVLSPKGERKIRQDSLDEIFSSLKKSPGGMHETNHTG
                     EGVERLSETKPYQYGDPPSNIDFMATMNNAMKRKRGEDIELREDDFEVYETEHKSTCA
                     TVLAIDISHSMILYGEDRITPAKKVALALTELILTRYPKDSLDVITFGDEAAQIDLED
                     IPYIHVGPYHTNTKEALQLARQILRKRRNQNKQVFMITDGKPSCIREEGKLYKNSFGL
                     DRKIVNQTLNEAAQCKKDNIVISTFMIADDPYLTRFVDTLTKTNKGRAFYAGLNELGQ
                     YIFVDYIKNRKKNVR"
     gene            complement(9683..10693)
                     /locus_tag="PK754_00615"
     CDS             complement(9683..10693)
                     /locus_tag="PK754_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HNB08036.1"
                     /translation="MQNNDINSFFPPFKAWFMVYRILAVLVWAVLTIVLSVSLAVYQT
                     GEIDWVNFLLTAAIASVVQGFPAHIVNEITDWKSGADQYRKLGEKSGGSKVLKSGLAT
                     ITQLWHIFDVTTIVALGLMFGLSQRTSPEIFWFFGIGYFVCLFYTLPPLQFAYRPFAG
                     EWFGGFTGIFLNMTGAYFVQTGQLDSAIILMAVITGLIYIAIMMVFHYLDYESDRHAT
                     PVKCTTIVFLGLKQSKFYVLGLLTLSFLLSSAAGWIIHPLFYLFSLLSLVHFGIHYVC
                     NPFEEASILKTGKRLTYLMIAFTVSISVVVHLYFIGLILFTVLCFVLHKKFGKLGPSH
                     AV"
     gene            complement(10696..11166)
                     /locus_tag="PK754_00620"
     CDS             complement(10696..11166)
                     /locus_tag="PK754_00620"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNB08037.1"
                     /translation="MKNHKDKYTHQAPVTDALFTLLTTRLANHPANSLLGFSLEELRE
                     LYACLRLTHREAITNGPRSHGTIHGGIVAALADTAAAFALSTAFEGKMSFATVDLHIT
                     YLSRAKSTIFAHAVVIRRGARINVCEVKITDESDALVATATVNFILTQPPETKS"
     gene            11323..11751
                     /locus_tag="PK754_00625"
     CDS             11323..11751
                     /locus_tag="PK754_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08038.1"
                     /translation="MTNQLRLNSSAGPSALFINSPWALIHRDEEVNAAMLEWKNLVTG
                     ETYKQILRQVLFLVRKENLSRCIWNLNSVGNINKEDLKWSFQFWLPAMAQCGVKSFAV
                     IVNEQHFLHLAIASYLEKQDIVEYGMFEKSASAMYWLQSH"
     gene            complement(11754..12365)
                     /locus_tag="PK754_00630"
     CDS             complement(11754..12365)
                     /locus_tag="PK754_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08039.1"
                     /translation="MEIRELTVDDIAQAARISVESFDDYNIVMSKMGEIYLRTVFFSA
                     IVQRQDTGAMGAFQDGKMLGYYCYMLDFHRFHADLQNKNFFRNVRFTLSAMMRGRLSI
                     KDLYNVFRAGAWIRRETAHIPVQVGPVAVARDIKGTPTGGMVTFTVVRAVLKKLQQQG
                     IKACWSTVDLRNPSQHVSTAVGFHEKSRIKLWGIEEALHVKKF"
     gene            complement(12438..12839)
                     /gene="mscL"
                     /locus_tag="PK754_00635"
     CDS             complement(12438..12839)
                     /gene="mscL"
                     /locus_tag="PK754_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNB08040.1"
                     /translation="MGMMQEFKQFASRGNVVDLAVGIIIGGAFGKIVSSAVSDLLMPP
                     LGWMIGGLSFTDLKFTFKTELLDVKPVTINYGNFLQTCVDFLIVAFAVFLLIKAMNRF
                     HQKQETAPAAPPAPSKQELLLEEIRDLLKKK"
     gene            complement(12872..13714)
                     /locus_tag="PK754_00640"
     CDS             complement(12872..13714)
                     /locus_tag="PK754_00640"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HNB08041.1"
                     /translation="MTSKFTLFALVMVVFAQMTHAQDTTWHRGGAASINFSQVQLKDW
                     GGGGDNSLALNSLLSLFANYHFEKTRWENSLELGYGIVKQGDAGTRKADDKIIYVSKY
                     NRDFVGPWKYSALLDFRTQFANGYDYSVPDSLRKPISKFMSPAALTIALGADYRPNDK
                     FSALISPVTGKATFVLDKQLSDLGSFGVKKGDKVYTEFGWFINSVYKTPLMTGIDFTS
                     KVNMFSSYKKIKEVDVNWENLILMKVNEYVSANLTAQFVYDKDVSNKWQIKEVFGAGL
                     LYKF"
     gene            complement(13796..15511)
                     /gene="sulP"
                     /locus_tag="PK754_00645"
     CDS             complement(13796..15511)
                     /gene="sulP"
                     /locus_tag="PK754_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HNB08042.1"
                     /translation="MLRPKLLDTLKDYNRERFIADLSAGTIVGVVALPLAIAFAIASG
                     VSPERGLYTAIIAGFLISFLSGSKVQIGGPTGAFVVIVYGIVEKYGIDGLIIATIMAG
                     VILILMGLAKFGGLIKYIPHPIITGFTSGIAVIIFSTQIRDFFGLTIDKVPGDFVEKW
                     ASYIEHGSSINFYALGIGIATLLIIIFWSRFSQKIPGSLAALIISTCVVYFFQLPVDT
                     IHTRFGEIPSSLPAPIFPNIQWSTITALIMPATTIALLGAIESLLCAVVADGMIGDRH
                     RSNMELIAQGLANIVTPFFGGIPATGAIARTATNIRNGGRTPIAGIIHALILLMILLI
                     FGKWAALIPLPCLAAILIYVAYNMSEHTAFMAIIRGPRTDVIVLLTTFVLTVLVDLTV
                     AIEIGMILAAFLFMKQMADVTHIQVIKSDLDDHDEPPDTQAIKDKRIPQGVQVFEING
                     PFFFGSVYKFQEHFDFARRHTRVLILRMRAVPYVDAGGLHALREMMNHCRKDKVYFLI
                     AEIHTQPLIAVEQSGLMQTMGEDYYFGNLDEALAAAGELIKSQPAENTESLHRYMHEI
                     REGQK"
     gene            complement(15531..16577)
                     /locus_tag="PK754_00650"
     CDS             complement(15531..16577)
                     /locus_tag="PK754_00650"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HNB08043.1"
                     /translation="MKKVKRWNALTFFKSTAVLFFFILLSACTPPSPIGPLPGKTPDS
                     FRIAFGSCNKTFLPQPLWNPIRAAQPDLWMWLGDIVYGDRGGIDTIRWMYNFQKNHPG
                     YAALRQTVPVIGIWDDHDYGVNDGGSEYEHKKETQRLLMDFLDEPAESPRRLQEGIYT
                     SYRYDKLNTRIKLILLDERSFREDPRANKDILGDIQWQWLYGELFGDTTDITLIASSS
                     QIIPNDNYAFNEVWQDYPASRDKMLSWLKTVPGTVLIVSGDRHYAEISQIENGRNNGR
                     STLTELTSSGLTHTKNYTLYSLLPIEPNSHRVGDFYGGLNFGVIDIDAVQRKIFGYVC
                     DHESMIRLQLVIDY"
     gene            complement(16584..17105)
                     /locus_tag="PK754_00655"
     CDS             complement(16584..17105)
                     /locus_tag="PK754_00655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019668833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNB08044.1"
                     /translation="MSSFFLRPSLLSDETAIYNLYNRVAAIRGGLAREAHEVTSDYVH
                     HFLSRSTATGLSIIAINENECLGEIHAYRPGIKVFDHVLSELTIAVDPKAQGLGIGKQ
                     LFTTFLDTVRTTMPNILRVELIARESNSKAIRMYEKLGFVTEGRFENRIRSVDGGLES
                     DIPMAWMNPNYKG"
     gene            complement(17105..18466)
                     /locus_tag="PK754_00660"
     CDS             complement(17105..18466)
                     /locus_tag="PK754_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNB08045.1"
                     /translation="MQSIQTILRRLTEAPDKEALYWNDRFFSYAELNTAIAAWEPVLK
                     DNGVGRGTVCAFLGDYSPQTAALIFALMKVKAVMVPFTYAIQHEIEEFKNIAGVECMF
                     RFAPDDTWTLERYNAVPRNALIEDFMQRDVPGLIVFSSGSTGKPKGILQDCERVMHKF
                     VEKRPGWRTLLFLMMDHFGGFNTFLSSFAYGGTAVCLSDRNPDTVCRIIQTSRATLLP
                     TTPTFINLLLASRVYGQYDLSSVKMITYGTEVMNAATLSKVRDIFPNAQIKQTYGLSE
                     LGVLRSQSESDDSVWVKIGGAGFEVKIVDNILWIRSEANMVGYLNAPSPFDNEGWMCT
                     GDQVEVRGEYMRILGRKSEMINVGGQKVFPVEVENVLMEAGNVREVSVFAKPHPIMGH
                     VVHAKITLHEPEDTLLATERLRKFCLERMAKYKVPVKFVFVDNSEQHNARFKKIRRDS
                     ESS"
     gene            complement(18471..18716)
                     /locus_tag="PK754_00665"
     CDS             complement(18471..18716)
                     /locus_tag="PK754_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNB08046.1"
                     /translation="MAKTTHDIKKVMARIFEVDPSSLHDTIRQDEVENWDSLRHMNLI
                     LGLEEEFGVEFTPEQSVEILSLPLIKVTLQELGVTFS"
     gene            complement(18718..20499)
                     /locus_tag="PK754_00670"
     CDS             complement(18718..20499)
                     /locus_tag="PK754_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HNB08047.1"
                     /translation="MMIPERLTDITLPDTLSETIALVNHLDHLEKKPEQSVRIHILRN
                     FTIEGIEPFLKLYHYQSGIRPEISFGNYDNVRQELLDKNSSLYQRHPDIVVLGLVLET
                     LDTNLRTFGWTADRVISEIESLLDTALQNTQALLAVNTFVPPWDSEWGMYRSPDAAER
                     FEEVQRINRKIRERVRRDPTRLYLMDWERYLIRLGEEAAMDYRFWYTSKAPFKKDFMS
                     AYAFDIAKLSRGLKGKTKKCLVLDCDNTLWGGIIGEDGVSSIKLDRDSFPGRVYRDFQ
                     LNALALYERGILLTLCSKNNEADVWEVFDHHPDMALRREHIAAWRINWTDKATNIRSL
                     AEELNIGLDSFVFVDDSPAECELVRTMIPDVTVLQVPQKLYTYPPFVIRSGFFDALQV
                     SSEDKNRTRMMHEESMRRVEETRYTSIDDYLASLQLYATIHEAPTDEIPRVAQLTQKT
                     NQFNVTTRRYSEAQITAFAENPDASVWTMRAGDKFGDFGLTAVIIVTRNGDTAEVDSF
                     LLSCRILGRKLETAFVRHVMAALTPRWNIRTWQAYYIPTKKNGQTEKFWDSFGFRPTP
                     QADRSVKYTIDASALDTTPIPFISYRS"
     gene            complement(20496..21683)
                     /locus_tag="PK754_00675"
     CDS             complement(20496..21683)
                     /locus_tag="PK754_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB08048.1"
                     /translation="MPDYTQIQIGDTAEIRHTITASDIEKFVALTGDDNKMHVDENFA
                     GRTSFKKPVVHGMLGASFISTVIGTKLPGDGALWFSQSLEFLLPVRIGDEITVRAEVL
                     RKIDAEQIIELQTDIINQHKQKVTTGKAKVKVIAQEKPSVAEETTVRKKVALIIGATG
                     GIGQATCRMLAKQGFDVAVHYFSNEKRAFDLVVSVKNEGRAAHAFKADIFKPEDIHHL
                     AEDVTRKLGTVTVLVNAATVKIPAIKWASVAWDDISQQWDINVRASFYLCKGIIPLME
                     KQKYGKIILLTTMYTESTPPTELMGYVTAKYALNGFAKALAVEMASKNITVNLVSPGM
                     TDTELVADVPEKVKLLNAMKAPMKRLAQSKDVAGTIAYLASDAADYITGETIRVNGGQ
                     TML"
     gene            complement(21733..22716)
                     /locus_tag="PK754_00680"
     CDS             complement(21733..22716)
                     /locus_tag="PK754_00680"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HNB08049.1"
                     /translation="MGIGITGVGKYIPSKEITNEQVEAWTGVSAADIVSKIGVTKRFV
                     VEAHETASGMSVEAARQAMVMAGITADQIGLIICATFTGDYLCPAMACRVQEQLGAKN
                     AGAYDVMANCTGFQIGLANAADRMTLDSSIAYALVIGTALQSRFINWKDPNSAIYFSD
                     GAGAAILARVPEGYGVLASDIMANGKVYDAVRVRGGGSSYPLRTENVNDVLPYFELNG
                     LEVWKQVVQFQPRVIMRVLEKISKKTDAVDFFIFHQANLRLIEFLMAKMKHPMHKTQT
                     NVETIGNTGEASMAIALCDAVLAGKLQRDQLVVISGVGAGFIFGATVLRWY"
     gene            complement(22737..23519)
                     /locus_tag="PK754_00685"
     CDS             complement(22737..23519)
                     /locus_tag="PK754_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB08050.1"
                     /translation="MPRPKAPASQAKSLIWVLWTDLGRKREYGMEGIYEYILDGFKIT
                     EVVTDPLWKENCYLITSASGEQALIDPGYDADRLADLISQNGDRGLTWVLLTHAHHDH
                     VGAVTPICRMFSIPAYLHKDDHKLIRHAPMYAIRFGRQPIPMPQPVNFFETPHVFELG
                     ALALRALHVPGHTKGGAAFVLGNALFTGDTLMHKHVGRADLPGSDMTMLKQSVDKLLS
                     EHHDTARIFAGHGKPWTIGEAREWWASAKNEPGVHDHFDTFA"
     gene            complement(23458..24162)
                     /locus_tag="PK754_00690"
     CDS             complement(23458..24162)
                     /locus_tag="PK754_00690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB08051.1"
                     /translation="MATPVMIITGTSKGIGKGLAEYFLNKGYRVFGCSRSEATITHAA
                     YHHTALDVSNESAVRLWIRTVKKEAGRIDILVCNAGLVESALQMTVTPGDVLEAYLKT
                     NVAGTYYVCRETAKTMLLQKQGRIITISSIMTRLHAPGTSVYSATKSAIEQMTKVLAR
                     EMAPLGITCNVVAPSMSPTDAAKSFGPEWEKNMLAQQTIQRPITTDEIANVISFYAAP
                     ESACITGQVTYMGLVD"
     gene            complement(24167..24466)
                     /locus_tag="PK754_00695"
     CDS             complement(24167..24466)
                     /locus_tag="PK754_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08052.1"
                     /translation="MNTEQILNVIQESFDSLTRSGLIASQVQVTPDLVILGNGSPLDS
                     MAFVTLFTDLEERIATASGKDIFLVLDEIEGFNINNPFLSAETIAGYIVGLIQKG"
     gene            complement(24547..25371)
                     /locus_tag="PK754_00700"
     CDS             complement(24547..25371)
                     /locus_tag="PK754_00700"
                     /inference="COORDINATES: protein motif:HMM:NF023385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNB08053.1"
                     /translation="MEKYFLFSIDLEDVRFWMSDGKKYRERVPAMTERYLKFLAQHRM
                     TCTFFVVGDVAEAYPSLIREIYSAGHEIACHTHRHIPLDQQTPETFRDDLLRNMDGLY
                     KAGVETLNGFRAPIFSLTKKTPWAYEILIEQGFTYSSSVLPATNPLYGWPEFGATFRR
                     VHDRIWELPITLFPSRFISVPCAGGLYFRTLPLWLTARAFRHHWNQSRPVLSYFHPYD
                     IDTEQERFMHPGLKDNRFYNWVMYQNRGTMLDKINTILADGATIIPYRDYITRHLQ"
     gene            25546..28704
                     /locus_tag="PK754_00705"
     CDS             25546..28704
                     /locus_tag="PK754_00705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB08054.1"
                     /translation="MLPFFIRHTLFWWVFVSHLFAQQYYIKNYSTSDGLPSNTIFEVF
                     VDCDYAMWIGTVDGLSYYDGQTFQNFNKTQGLINHRVWSVAQDSSGNIWVGTRTGINR
                     FHKTRSGWQIRAWLTQLSVRHIHIETDQSMILSTENGAYKFTCFTDDSSSLVPIEGLQ
                     GKMVYQTLRDPDNRLWIATDQGVWIQKGTVLENISTPFPIVRGLFLRDSSHLYALHFR
                     GGISLTDPRSDSPSFVSQDLPKGFTKRSFYRMIVDPTGNFWAATDLGITRLRFSDTRV
                     VSMEVIDETKGLANNLTIPLVSDHEGHIWVGTHNGLSRFAGLRFSYFIPHNNPGIQVY
                     KSIAASGTDIYMLTSAGLFYVDAQHPAVPLPLYGNTPIQEGVSLGKNRLALVNDSEIA
                     IFFVGGKTFRKQKKISVEGQSIASFLFLDEQDVMIGTSAGLYRHRHGRVTRVNASTFT
                     DQMMVYTLMRDDKGAIWVGTSHGAYVLTADQDSLRVQSWSLPGVMPFQAVGQIYRDAS
                     GMMWFCTEGAGLARWDGQNVRVYTTDNGISNNIVYSVFEPDTTTLWVCTAGGIDVMNR
                     KNDSWMHWDSHSGFFRKGSTPYSIMKSSDSVYWISTSRGLVRTDFSEPFASGNSVPVL
                     VDQILVQGKPQRHSGILRIEPGVQQIQFRIKAISYRYENDVRYSFFMEGFDEAWSEPS
                     SQGVIQYAGLSPGIYRLKSKAITPGGQYRGPDVVLEVLTPFWKETWFVAISCLVLIAV
                     TAGAYRWRTYHLWKKARQLEMLVRDRTKDLVRKSRDVIKQKKELEKTLQTLRTTEAQL
                     IQAEKLSALGELTAGVAHEINNPIAFIYGNLPLLEKDLRRLTELAQKAKTGPLDDKEA
                     LEMTQIENDIHSAIVSTRHGVQRVQVIVKTLKKFVRLDEAKEDFKSVEECLDSAADAI
                     IIQYPKVQIKKDYRETPQILCYPSELQQAFYHVMLNAIQSQATDARVDFSTSTDGKYV
                     YVGIRDYGTGIPEAISQKIFDPFFTTKDVGRGKGLGLSMTYGIMEKHEGGIRFENCQD
                     QGAKFILMLPVERRSSSPSVLS"
     gene            complement(28771..29892)
                     /locus_tag="PK754_00710"
     CDS             complement(28771..29892)
                     /locus_tag="PK754_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNB08055.1"
                     /translation="MSTHAKILVVEDDFQNRQLIQIYLSRMNFDVTLAPNGFAAMEKL
                     KTYKPDLIISDISMPYMDGFELYNNVKAIPDLRSIPFIFLTAHSDADKRRYGKEIGSD
                     DFLTKPIEQEDLVASIRGKLKRVEEIRSSTEAEMLTQIDQLKREILSTITHEVNTPLF
                     IIKLTANLLLDETMEFKPHELQELLLRIKKSGERLDALLKDFLITVRIAAGDAKSEYK
                     EAKQDSDINFIVEHLVPQFKKRAEIKQLRIQSQLERHLPGLQLHVDQVADVVERLFSN
                     AYKFTPPGGEIRIRSGKDHDYVRLDIEDTGIGIPEEQLDRIFHKFYQVNRAEMEQQGA
                     GLGLAIARDLTEINGGRLVVKSHEGMGSTFSLLFPIHVI"
     gene            complement(29897..32029)
                     /gene="metG"
                     /locus_tag="PK754_00715"
     CDS             complement(29897..32029)
                     /gene="metG"
                     /locus_tag="PK754_00715"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNB08056.1"
                     /translation="MPTDTSFKRILVTSALPYANGPSHIGRLTGAYLPADIYVRYQRL
                     MRRDIVYICGSDEHGVPITIQAEKEGVTPQALVDRYHAMIQDEFSRMGISFDHYDRTS
                     NPVHHDTAQEIFLDLLEKNLLKRKPEMQWYDEQAKMFLSDRYVEGTCPVCQNTDARGD
                     QCEKCGTYLNQMDLIQPRSKVTGTTPIAKETTHFYLPMGEFQSELEKWISTKSHWKDN
                     VINYCKGWFAQKLGDRAITRDIDWGVPVPKKDRKNNAIEGSDRKVIYVWFEAVLGYIS
                     STKEWAKKVGQPEKWRDYWQKDDTQLVHFIGKDNIVFHAIMFPIITMAYNQSKTSGRL
                     VLVSDVPACEFLNLEGNKLSTSRNYAVWVKDYLQKFPPDLLRYTLTSNLPENKDSDFS
                     WKDFQIKNNSELADILGNFINRSLSFTIRNYHNKVPSRGTLTAMDEQMLSEILAAQTE
                     VGKFIEEFRFRDALKRFMDLARAANKYFNDTEPWKKIKTEPERGATILWVSVQVVRAL
                     AVIMHPFLPFTSEKVWQMLGLSGLVKEQDWNTIGTSRMDTGHVLGKVEIVFPKIEDDV
                     IQKEIDHLNSMIEQKTTESAQSKPVEKPKSENEAVTAAQISIDEFGKIDMRSAKVLHA
                     EKIEGTTKLLKLQLDLGFEKRQVVSGLAEYYKPEELIGKDVIIVANLKPAKIRGVESN
                     GMILTVSDGTTLKILSPEAVMPGGLRVK"
     gene            complement(32072..32887)
                     /locus_tag="PK754_00720"
     CDS             complement(32072..32887)
                     /locus_tag="PK754_00720"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB08057.1"
                     /translation="MGLEVTFYGTRGSISATHEDSLKYGSHTACVVISSGERSVIIDA
                     GFGIGFYSDHLKNKMGEFHLLISHFHWDHIQGLNYFGAMHHPKSINHFYSPQSNDKLK
                     EVMDIYFDGSYGPFNGWQQLNSKLEFHQLTQDIEINGFQISCRALNHTDPCFAYKISD
                     GGHSVVYASDHEAIDNSVNREFITWAKGADMLIHDAMYLDEEYAQRVGWGHSTFDMAC
                     RNAQQIECKHLLLTHHEPMRTDRELDRIELRLKEKYSDLRLTCAQQEVVYVTK"
     gene            complement(32878..33780)
                     /locus_tag="PK754_00725"
     CDS             complement(32878..33780)
                     /locus_tag="PK754_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB08058.1"
                     /translation="MKFTLLGTRGSRPILTPDRVRYGGNTTAFKITIDGMQPIYIDGG
                     TGIYREGVTISEQHPEGFHAHFFITHTHWDHILAFPLFAPLFNKNTKISISGPRSEEY
                     TIEELFKLQHAKGLIPIPFSLVRDKITFHELHPDQTIPLENATVKTVQLNHQGLTLGY
                     RIEHGGKSVLIITDHAPIENNHLGVLMKAWNKEDFTKTEKEFYENMVRFCTGADIMLH
                     DTHFTKEGIKGKENWGHSTPEMAVDLAVKAGVKKIILGHHAPEDTDTFVEQKITSAND
                     YVKSIGKAGAIEVVALREGTEVWV"
     gene            complement(33898..35472)
                     /gene="pckA"
                     /locus_tag="PK754_00730"
     CDS             complement(33898..35472)
                     /gene="pckA"
                     /locus_tag="PK754_00730"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNB08059.1"
                     /translation="MTTNNELAHLGIVNTGTIHKNLSVSQLVELAIKRGEGQLASNGA
                     LVTNTTPHTGRSPKDKYVVEEPTSKDKIWWENNKKVSQAQFDVMFNRMRSYLQGKDVF
                     VFDGFAGADTKNRLSVRVISEKAWHSLFAKTLFIRPTEAELATHHADWTVIDVCGMHA
                     IPEFDGLGTKTFIMLNFEKKIVLIGGTHYAGEIKKSIFTVMNYILPERGIFPMHCSAN
                     SNKEGEVTLFFGLSGTGKTTLSADPLRRLIGDDEHGWSNEGVFNFEGGCYAKTIKLSK
                     EGEPQIWNAIRFGSVLENVVLKQDGTPDYDSAAIAENTRVTYPVEYIDNCIIPGIGGH
                     PKNICFLVCDAYGVLPPISKLTPAQAMYHFLSGYTAKVAGTEAGVTEPTPTFSTCFGA
                     PFWPLHPNVYAELLRKKLNEHNATVWLVNTGWSGGGYGVGSRMKLSYTRALINAALSG
                     KLNNVKYNNDPVFGVAVPESCPDVPAEILVPKNTWKDKAGYDAQAKKLADLFIKNFEK
                     FADKASDDVKKAGPKA"
     gene            complement(35512..36501)
                     /gene="ricT"
                     /locus_tag="PK754_00735"
     CDS             complement(35512..36501)
                     /gene="ricT"
                     /locus_tag="PK754_00735"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNB08060.1"
                     /translation="MQNPIQVEFKANRKEIFLNKNNLVVKVGDHVIVQCENGVDMGKV
                     ALTHEQIPEDLTKDKELRQFIRVANPDEISKLADNRNEEEKTKAVVKQKIIKHGLKMK
                     LVDAEYQFDRKKIVFFFTAEKRVDFRSLVKDLAGEFKTRIELRQIGVRDEAKRVDGYG
                     PCGKQLCCSGHLTEFQPITTEYAKDQGLQLNPSKLTGLCGRLKCCLAYERDFYVNTLS
                     RFPGVGSPIKTKKGRGEIKHLDIIGNKVHIKQDHDNEWETLTLEDVADILGIDMSDIH
                     GCGSGCASGGDGCQSGKCNTHDHVEEGTMQNPLKDFMENDPFFDQYNFPRPKI"
     gene            complement(36547..37686)
                     /locus_tag="PK754_00740"
     CDS             complement(36547..37686)
                     /locus_tag="PK754_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HNB08061.1"
                     /translation="MLSKVIGQEAAKKQILTAFANDRMAHAYLIAGDEGLGAEELALE
                     ATRFILCKHPDRERWEPCGECANCKKMATLQHPDVHYYFPVMRATEEDEQRRLLEEKT
                     RNLYARVRIANGSIPIGDPENPEVNSIRWLSRAISLRSFEGELKIFILTFIEDMHAEA
                     TNAVLKILEEPPASTLYFLTTTNIHSLLPTIISRCQIVKLHRLTDDQLLDGIRRHYDV
                     AENQAKFISRLANGNYYQASELISGDFPRKREAMLEFLLAAMSNKSASIQTIIEQWNE
                     EAEKDKSFYTDILTMLITWFQDVVYMRELGEDKSLIDRFLINEDKTERLEKFIKAFPR
                     VRVEEALSEIEKAVDLIGRNIYINLILMNLGLNLRSIVNPSSGKS"
     gene            complement(37873..>38167)
                     /locus_tag="PK754_00745"
     CDS             complement(37873..>38167)
                     /locus_tag="PK754_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08062.1"
                     /translation="VHEEIRKLAENGPAESDVNKIREALRRDLERNQKENAYWLRWIE
                     NAYYENASIKNITPEAQEKLIRRLSAKDIRKIAQKYIQNGHALIFKMRPEEKK"
BASE COUNT         9917 a         8660 c         9047 g        10543 t
ORIGIN      
        1 ttctactgca ttggcgggtg cttcgcggga tttttcagaa agctcttcca atattttatt
       61 tgcattcatt ttggccctgg tactcattta ccttgtgctg gccgcgcaat ttgaaagttt
      121 tgtggatcct tttattatca tgctgacggt gccaatggca cttgccggcg cgattttgtc
      181 gctgtggtgg tttcagcaaa cactgaatat atttagtcag atcggcatta tcatgctgat
      241 cggtctcgtg acaaaaaacg gaattctgat cgtcgaattt gccaatcagc ttcgtgaaaa
      301 agggaaaaac aaaatggatt cgattttaga ggcgtccgtg ctgcgtttca gaccgatttt
      361 gatgaccagc cttgctacgg tgcttggtgc cgttccgatc gctttagcgc taggcgcggg
      421 ttcgattagc cgaattgcca tgggtattgt gattataggc ggtgtgattt tttcgctgat
      481 tctgactctg tatgtgattc ctgcgattta ttcttatatg aagcatgagc acaagcccgg
      541 tcaggaaaca aatatcgctc cattgcaaca aacagaatcg ttatcaaagg agcatgtata
      601 gaaaagtgtt taggtgtaat agataaaaac aaaacccggg gcattaaatc tcccgggttt
      661 ttttaattct acaacatcaa cggaataccg ttttttttaa aatcgtttcc atcaaacacc
      721 atccggtaaa tgatgattgg atgagattga tacctacgaa cgccgtaaag aacagccagt
      781 tgggatgaac ccaataacct aaggccagac taatcagtac gaaagtaccg gcgataacac
      841 gaatggtttt ttctaacata gtatctcctt atgcaaattg ttcgacatgc atttgaaagg
      901 cgcgcatcgg atgatcgaga tccatcgttt gtccatgatt gcggatggct tgcatcagtt
      961 cagcagcctt tgattcatca acaaaagcaa aaaccagatg cgaataaatc ggcgaagccg
     1021 tgtgtgcaaa ccaattggaa gaatcgcgct ccgtagcatc actccggtag ccacgaattt
     1081 ccgactctga aaaaatgatg acgtgatgtt cgcgaaaaag tttacgcaga tcctgcgcgt
     1141 actgttcgat cgtcatgact gcgatcagtt tcatatcaat tctccttcga ttcgttttcc
     1201 gttgtttcaa gaggcacact cgcttgcggc acagcctcgg attcgtactg cgctttttcg
     1261 gtcatataat aaaaaagcgg caccatgatc agtgtgaggg ctgtcgaagc gatcgcacca
     1321 ccgataagcg aaatagctaa cccttggaaa atcggatcaa ataaaatcac aaatgcgccg
     1381 atgacaaccg cgcctgaggt cagaagaatc ggcgtagtac ggacggcgcc ggattcgata
     1441 atggccgttt tcagcggcac accgtcctgc aagcgcaatt cgatgaaatc aatcagtaat
     1501 acggaattgc gaaccatcac gccggccaat gcgatcatac cgatcatcga agtggctgta
     1561 aaaaaggcat ccatcatcca gtggccgacc agaataccta ccaatgacag cggaatcgca
     1621 accatcatta caaaaggtac tttgaaggat tgaaaccaac cgatgatcaa aaaatagata
     1681 atcacgatca cggcggcgaa ggcggcaccc agatcacgga acacttcgta agtgatctgc
     1741 cattcgccgt cccacttcat cgaaaaattt tcttcaagga aaggttgttt ggcgtatagc
     1801 gtttgcagag tgtacccttc aggtaaccgg atttttttca tcgcttcgtc gatgtttaaa
     1861 atcgcatata cagggctttc cacttcacct gctacatcgc ccgttacata aaccacgcgt
     1921 tgctgatttt tacggtagat acttttttca cgcgtttgag gatagattct gaccaagtcc
     1981 gataccgcta caggaatgcc tgtcatcgcg atgacatgga tgtttttgag gtcatcaacg
     2041 gacgaacgct gagattcggc gagttggagt tgaatgccta cggtttccag ttctttggcc
     2101 tgatacagtg cgccgatttg tttcccgttt aatgcaattc ctaaaacgtg ataaatctgc
     2161 tgcgggctga caccgctttg tgccgctttt tctttgtcaa tttcaaatcg gtattcggtt
     2221 tgatcgtctt cgacaagcca atccacatcc actacgccgg gcgacgtttc aaacgccgta
     2281 cgcacctgat gggctacctc aatttgttta gtataatccg gcccatatac ttcagcaacc
     2341 agcgtggaca ataccggcgg gccgggcgga acctctgcaa ttttgatgcg tgcgccatag
     2401 gaagcgccga tctttacgat agccggtcga atacgtttgg caatgtcatg gctttgttcg
     2461 ctccgctcgc ctttgggaag aaaattgact tggatatcgg cgacattgga gccttgtctc
     2521 agatcataat ggcgtacaag cccgttgaaa ttgatcggcg cggaagtacc ggtgtacatc
     2581 tgataatggg ttacttccgg cacgttacgc agataggcgg caatctcttt gctcaacgca
     2641 gccgtttttt ctagcgtggc gccttccggc atgtcaatta caacttgtaa ttcattttta
     2701 ttatcaaagg gtaacatttt tacggttacc aatttgaagt acatcagaga catggaagcc
     2761 agcaatagca cgacgacact tccgatgaac caccagcgtt tttttgcgtt ttcgagtaac
     2821 ggaacaaaaa tacgggtata tagtttataa attcccgtcg tttcaaggcg atattctttc
     2881 tccgaatgcc cgtgcgtatc gccgcgcaaa agacggtagg cgaggtacgg cgtgatgatc
     2941 agcgcgacga acagtgaaaa cagcatcgcc agcgaggcgc cgatcggcat agggctcata
     3001 tacgggccca tcaatcccga cacaaaagcc atcggcagta cggaagcgat caccgtaaac
     3061 gtcgctaaaa tggtgggatt gccgacctcg tctatcgacg caatagccgc ctgtaaaaac
     3121 ggcaatttcc gcattttgaa gtggcgatgc atgttttcaa ctacgatgat ggcgtcgtcc
     3181 acaaccaatc ctgtcacgaa caccaacgca aaaagtgtaa tacgattgag cgtatagcca
     3241 aacatgtagt acacaaaaag tgtcatagca aaggtcaccg gtacggtcat aaaaatgacc
     3301 aatccgctac gccaccccat ggtcaacgca acaagtaccg tcacggccaa aatagctgca
     3361 gaaagatgtt tgagcagttc gtgtactttt tccgacgccg tttcgccgta attgcgcgtg
     3421 acggtcatgg ttacgtcgga gggaattaat ttgcccttca atgcttctga cttttccatc
     3481 aggcgttcgc cgaggcccat cgcatcggag cctttacgtt tggataccga caacgtgacc
     3541 gccggatatt ccaacgcttt atgttcaccg gcgtgagcgg ccccgtaacc gaacgtcaca
     3601 taatctttga tttcatccgg accgtcttcg atctgagcga tatttttgag atagacaggt
     3661 ttgcccaggt gcatgccgac gaccaattgg cgtacgtcat ctgcggtttc tagaaatttt
     3721 cccgtttgta ctaaatattc cgtgttgcct tgattaaatt ttcctgccgg gccttgttgg
     3781 tgggcggatt ggaaaaaagg gataatcgtg agtggatcaa ggcgatacgc cgccattttt
     3841 tctttgtcca atatgacccg cacttgccgt gagcgtccgc cgataatttt cacttcggac
     3901 acgtcgttga tttttttgta ttcagaactg agttctgcag caaaacgtcg taaatcaagc
     3961 gcatcatacc gggcgctcca tagagtcatt gcaaaaatcg gtacgtcgtc aaccgatttg
     4021 gatttgatga acggcggcct gacaccggcc ggcatcttat ccatgttttt gaaaacttcg
     4081 tcgtagagcc gaatgatcgc gcgctcggca tcctcaccga cgacaaaacg tacgatgagc
     4141 atgccttggt cctgcatgga cgtcgagtac acgtattcga cgccgcggat attggaaatt
     4201 attttttcca gcggctggat gactttggtt tctatttctt taggttcagc gcccggatac
     4261 tgcacaaaaa catccgccat cggcaccacg atttgcggtt cttcttcgct cggtgtgaga
     4321 taggtggcaa aaagaccgat tgctaaaaac cctgcgatga gcaacggcgt cagtttagaa
     4381 ttgataaacg cttgtgcgat gcgtccagaa aatccggttt tcatgggcga acctccacac
     4441 gatcaccgtc gctcaggcgt tccgttgatt cgtatataac acgatcaccg aaatcaagtc
     4501 cgctcaatac ttctacttca ttgccatagc ttttacccgg gcgaatccat cgtaacaaag
     4561 cctggttggc tgcgctgact acgaaaacac cttccagttg gccgcgccga tgtatcactg
     4621 aatccggaac agtgatcata tttttttgac cggcgcccgc atagatgcgt acgaacatgc
     4681 cggagcgcaa cgttttcttc aggttttccg ttattccatg aagcttcact tccgcgtcat
     4741 attggcgtga gccgggaact gcagaaggac tgagggcgct gacatagccg tttaaccgat
     4801 gcttcggatc tgaagaaaac tcggcttcga cggcatcctg gatacgaatc gaagcggcgt
     4861 cgctttccgg gatcgggata atgactttga aggatgaaag atcctcaata accatcagag
     4921 gcatacccgg tgcagcggta ttgcccactt cagtgagttt ttggacgacg taaccgtcga
     4981 tgggcgctgt aatatccgaa tacccaaaca cgtcgttgat ttctttttcc atttcttcga
     5041 cggctttaag tttggcctgt gtcatcgcgt acgccatttc cgtgtcgtcc atttcctttt
     5101 gtgtagcact tttgcgggcg tataattctt taatgcggtg gtaattggat tccatatttt
     5161 ttaaagcggc ttccgcttcg attttattgg cggcgatttg cgcgcgtttg gcgctcaagt
     5221 catcgctttt gattttgatc agcagttggc ctttagcgac gcgatccccg atgtgggcat
     5281 gtattgccag gacttgaccc atgattttgg tgctgagcgt aatgcgttta tcgccttgca
     5341 atgtaccggc caaggcggtt ttttcgttaa ccggtttttg acggatcgtg attgtgcgca
     5401 ccggtgttgc ggtttgattg ggttgatttt tctccggaga accgcatgcg accataacga
     5461 tcatcatgac ggacaaactt aacccgataa aacatttctg catttttttc ctcgtgtata
     5521 ttaatttttt atggattgtt cggtgagtaa ttcgaccgta aacatcgctg catgccaggc
     5581 aaaccgggca tgtatcaatt ggaggcgtgc ttgcgataaa gccgcttcgg ccatcagcaa
     5641 atccgttgta cgatctaaac cctgatgata gcggtcggaa agaacacgat aattttcttc
     5701 ggattgctgc acggcctgtt cagcgataac gacttggcgg gttgaaactt tccactgacg
     5761 aagtgccgcg ttaaggtcat attgactttg cgtcagcgct tttttataac cggtttcggt
     5821 ttgacggtat tgcgcgtttg atttctgaat agcggaaatt tggtcgaaac cggagaaaat
     5881 atcccagcgc agcattgcgc cgaccatcca gtttttagct tgccggccga acagggatgc
     5941 gtcattgtat tcgtaagtgg caaatccatt gatactggga agaaatttac tccaggccat
     6001 actttgcatg gcacgctgtg cgctcatctg ataacgcatg gctcgtatat cgctacggtt
     6061 tgtcatatcg tgtggcgttg tcatttctaa ggctaatggc ccgcccagtg tgtcggtagg
     6121 aataatcact tcatcgcctg acaaacccat cagaagtttg agctgattat tcgcattatc
     6181 tatcatgttt tctgtttcaa gttgtttagc ttccagttct tttaaacgga gctcggcaaa
     6241 aagtacatcg gagcgacgga tcagtccttg atcataaaaa ttgcgcgcct gctcgctacc
     6301 ggtctgggcg gcagtaagcg ctttgcgaat aacttcacgg ctttgcaaag cgactaaaag
     6361 ttggtaataa gctattttgg cttgataggt gatatagtaa gatgtgcgtt tgcttttttc
     6421 ttccatcgct ttgacgccgg aggatgccgc acgaaaaccg aaaaaaccgt ccatattcag
     6481 cagtggttgc cgccattcca gtttggtggt ataattatct acagctttag gttgattgag
     6541 tgtgaccgga ttgaaatccg cctgagttac aatttcttgt ttaagtttta aaccgaaaac
     6601 atttaacgga tcattggtgc gggtataaat ttccgaaacc gataattgag gaagaaaaag
     6661 agacaacgat tttcggtaat cggctctcat cgcatccatt tccgcttgta cagaagctaa
     6721 atcataattt tggcgcgaag cggtttgtaa tacggagtcg agagtgtatc gggtgccgtc
     6781 ctgcccggcc ttgacttgtc cgaccagcac aaacaaaaga aaaactccaa aacataaact
     6841 tttcatacta ctccttaaac ttaactatat aaccatatag taatataata aacttttaac
     6901 tccaagtcaa gtattttatt ccgccgtttt ttagatcgtt aattcactat ttacattagc
     6961 cgattacaga aatacattta taaaacatcc ttaaaggtac gctcatgtat tatttgttga
     7021 tcgcactggt tttgttaaca aaagctgttt tggccgggga tgcgccggat tgggttattc
     7081 aacccggaaa atcagccgca tttcctgcat ctgcttatat agtaggatac ggctccgctt
     7141 ctgcccaaaa cccggacgca caaaaaattg cggaagataa tgcacgagcg gccttatccg
     7201 gttcgatcgt aaccgatctg cgcagtacgg taatttcgag gcttgaagag caagggaaaa
     7261 attcggcgga atatttcagt tcggttaccc agtcttctac cggattacaa ttgttgggca
     7321 tttcgataga aacatataaa gataaaaaac tggttcatgt gttggcgtat gccaaacgaa
     7381 ccgaacttag cgccacgtac aacaaaaaac gtgaggcgtt aatttcgaat atcgctgcga
     7441 tcattacaca agccaaggcc gcagaagaat caaaaaaaat agaagatgct gccgcacttt
     7501 atttgagttt gtttccgatg tatgaagagt tgcgcgaagc ggagatcgta ttgacggtgg
     7561 ttcatcgcga aagccaagtt tctcaaccgg ataagactat cccgtcccgt acagagatag
     7621 ctcaaaccat agatcgcctt ctggcgcaaa gcattacatc cgtcgaagat gcggcacgag
     7681 ccgttgctta tcaggtatcc aaacaaatat ccggcacgac cggatcgttt ttggtgtcac
     7741 cgatgacgta tcaagattcg aaaatgagta gtcccttcgc acgttatttt caacaagcac
     7801 tggagacgca atttcaaaaa tatgcggtat gggaagttgc acggcaggca cgcggatttc
     7861 aacccaaatc atctcagatt acccgtgatc ttgtcattgc atccggcgcg ggatgtattt
     7921 ttgagggcaa ttattgggag caaggcgatc gcatcaaact aattggtcgt ttgcgcgatg
     7981 ttcaaaacgg gcgcatcatg gccgccgtag aagtaacgct ggatattgcg catattcaga
     8041 aaaccggtct ttcgatcaaa ccggaaaata tgcttaaggc cttagctgac caaaaggctt
     8101 ttgctgaaga agaagtgatc agttcggata tccagttgga tgtgtggacc aacaaaggca
     8161 cggaaaacct tctttttgct gaaggtgaaa tcatgaagat ctacgtgcga accaatcgcg
     8221 ctgcgcacat acgcattttg tataattttg ctgacggttc acgaacactt ttatatgatg
     8281 atcattatat agatgaagct aaggccaatc aggtcgttga aatacccggt gaatttgaat
     8341 gtacgggacc ttttggtgct gaaatgatgg tcgtcgttgc cagcgtagga aaactaccgg
     8401 aagtgaagac gtacagtgtg gatggtatcg cgtatctgga tgaaaaagat gccaaaaaag
     8461 cggcatatgg tacacgcggc ttcaaaaaga aagatccggc atcttccact agtcctcaac
     8521 aaaccgaaaa aaaactgacc atcactacta tcgcaaaata atctagttta gcagcacttt
     8581 cgatttcatc ctaaactatc gaacattctt ttttcggttt ttgatgtagt ccacgaatat
     8641 gtattgcccg agttcgttga ggcctgcata aaacgcacgg cctttgttgg ttttggtgag
     8701 tgtatctaca aagcgtgtca ggtaaggatc atcggcgatc ataaatgtcg aaatcacgat
     8761 attatctttt ttgcattgtg ccgcttcgtt gagcgtttga ttgacgattt tgcgatccaa
     8821 accaaacgaa tttttataca gtttaccttc ttcgcgaata catgacggtt tcccgtcggt
     8881 aatcataaaa acctgcttgt tttgattgcg tcgctttcgc aggatttgcc ttgccaactg
     8941 gagcgcttct ttggtattgg tgtgataagg gccgacatga atatagggaa tgtcttcgag
     9001 atcaatctgt gctgcttcat cgccaaacgt aatcacatct agactgtctt tgggataacg
     9061 cgtgagtata agttccgtca acgccaaagc cacttttttg gccggagtga tgcggtcttc
     9121 accgtataaa atcatgctgt gactgatatc aatggccaat accgtagcgc aggtagattt
     9181 gtgttcggtt tcatacactt caaaatcatc ttcacgaagt tcgatgtcct ctccgcgttt
     9241 tcgtttcatg gcattgttca tcgttgccat aaaatcaata ttggacgggg gatcgccgta
     9301 ctgataaggt ttggtttcgc tcaagcgttc aacgccttcg cccgtgtgat tcgtttcatg
     9361 catgccgccg ggcgattttt tgagagatga aaaaatctcg tccagagaat cctgtcggat
     9421 tttccgttca cctttaggcg aaaggacggg agcgctgcca tcttgggatt cttcgatata
     9481 cccttcctgg acaagacgat cataaaattt ctgaaaatcc atatcttcgt taaagatatg
     9541 atactgacga tcgatctgcg tcatatagct cagagctttg cttacgtcac cgtccgttag
     9601 cagtaagagc tgattaaaga ttttcatcag atcgtcgaac ttcattttgt gattcgacgg
     9661 tcccggcata aattgtgaat attcaaaccg catgagaggg acctaatttt ccaaattttt
     9721 tatgcagtac aaaacacaaa accgtaaaaa gaatcagacc gatgaaatac agatgcacaa
     9781 caaccgaaat actgaccgtg aaagcgatca taaggtaggt taggcgtttt ccggttttta
     9841 gaatggatgc ttcttcgaac ggattacaaa cataatgaat gccgaaatga acgagtgaaa
     9901 gcaggctaaa taaataaaac aacggatgaa ttatccaacc ggcagcagaa gataacaaga
     9961 aagacagcgt caataatcca agcacataga acttgctttg ttttaagccg agaaagacta
    10021 tggttgtaca ttttacaggc gtcgcatgac gatcgctttc gtagtcgaga taatggaaaa
    10081 ccatcataat cgcgatataa atcaatccgg ttattacggc cattagaata atagcgctat
    10141 ccaattggcc ggtctgaacg aaataggcgc cggtcatatt gagaaaaatt cctgtaaaac
    10201 cgccgaacca ttctccggca aaaggtctgt aagcaaactg taacggaggc aatgtgtaaa
    10261 ataaacacac gaaatatccg attccgaaga accaaaaaat ttcaggtgaa gtgcgttgcg
    10321 acagcccgaa catcaaacca agcgctacaa tcgtggtcac atcgaaaata tgccataatt
    10381 gggtaatcgt ggccaagccg gatttgagca ctttactgcc gccggatttt tcaccgagct
    10441 tgcggtattg gtcggcgccg cttttccaat cggtgatttc gttgacaata tgtgccggaa
    10501 atccttggac aacagaagcg atagcggctg taagtaaaaa attgacccaa tcaatctctc
    10561 ctgtctgata cactgccagc gatacgctga gtacgatggt caatacggcc cataccaata
    10621 ccgccagaat tcgatacacc ataaaccagg ctttaaacgg agggaaaaaa gaatttatat
    10681 cgttattttg catggttatg atttggtttc cggaggttga gtcaatataa agttgaccgt
    10741 cgccgtggcg acaagtgcat cggattcgtc tgttatttta acttcacaaa cattgattcg
    10801 cgcgccgcga cggatgacga cggcatgggc aaagatagta cttttagcgc gtgaaaggta
    10861 ggtaatatgc aagtccaccg tagcaaaact catctttccc tcaaacgcgg tggacaatgc
    10921 aaaagccgcc gccgtatcgg ccaatgcagc gacgatgccg ccgtggatcg taccatgact
    10981 tcgcgggcca ttggtgatag cctcacggtg cgtaagccgc aggcaggcat acaactcgcg
    11041 tagttcttcc aaagaaaatc caagcaaact gttagccgga tggtttgcta aacgagtagt
    11101 cagtaatgtg aaaagcgcat cggttaccgg cgcttgatgg gtatatttgt ctttgtggtt
    11161 tttcatgttg agtgtttaca gcgcatataa gaataacata aaaaactatg gtaatttggt
    11221 ttagaaatca aattttcgcc tacagattca caaaacgtat ttatgcgtac ccacacaatg
    11281 atgtaggttg tttcgccgag aggtttgatc catattaata caatgacaaa ccaactgcga
    11341 ttaaattcat ccgccgggcc gtcggcatta ttcatcaatt cgccttgggc tcttattcac
    11401 cgagatgaag aggttaatgc cgccatgtta gaatggaaaa atcttgtaac cggtgaaacg
    11461 tataaacaga ttttacgtca agtattgttt cttgtgcgta aggaaaactt gtcccgttgc
    11521 atttggaatc tgaacagtgt tggaaatatt aataaagaag acctgaaatg gagctttcag
    11581 ttctggcttc cggcgatggc ccaatgcggt gtgaaatcgt ttgccgtcat cgtcaatgaa
    11641 cagcattttt tacatttggc cattgcttca tatttagaaa aacaagacat cgtagaatat
    11701 ggtatgtttg aaaaaagcgc cagtgcgatg tattggctac aatcgcattg aaattaaaat
    11761 ttctttacgt gcaacgcttc ttcgataccc cataacttga tacgtgattt ttcgtgaaat
    11821 ccgacggcgg tagatacatg ctgggaagga ttgcgcaaat ccacggtact ccaacacgct
    11881 ttgatccctt gttgttgcaa cttttttaat acggcgcgca cgaccgtaaa ggtcaccata
    11941 cctccggtcg gagtgccttt gatgtcacgc gctacggcaa ccggcccgac ttgcaccggt
    12001 atgtgggctg tttcacgccg aatccatgct ccggcacgaa atacattata aagatctttt
    12061 atgctcaacc gtccgcgcat catagccgaa agcgtaaaac ggacgtttcg aaaaaagttc
    12121 ttattttgca gatcggcatg aaaacgatgg aaatcaagca tgtaacaata ataaccgagc
    12181 attttcccgt cttggaatgc acccatcgcg cccgtatctt ggcgttgaac tatggcggaa
    12241 aaaaaaacgg tccgaagata aatttctccc attttgctca taacgatatt gtagtcgtca
    12301 aacgattcga cagagatacg cgccgcttgt gcgatatcgt caacggttag ttctcggatt
    12361 tccatagttt tgcgaatgcg gttttaaaat atgagggagc ggcacgaatc cgcccccata
    12421 caatgagtcc aagtttttta tttctttttg agtaagtctc tgatttcttc gagcagcaat
    12481 tcttgtttgc ttggcgccgg aggtgcagcc ggcgctgttt cctgtttctg atgaaagcgg
    12541 ttcatcgctt tgatcagaag aaacacggca aaagccacga tcaaaaaatc aacacacgtt
    12601 tgcaaaaagt ttccgtaatt gatggtaacc ggtttgacat caagtaactc cgttttgaac
    12661 gtaaatttca aatccgtaaa actgagcccg ccgatcatcc atccgagcgg aggcattagc
    12721 agatcgctca cggccgatga aacgattttt ccgaaagcgc cgccgatgat gatacctacg
    12781 gcaagatcga ccacattgcc acgcgaagca aattgcttaa attcctgcat catacccata
    12841 caatattctc ctcgttatgt tggcagagcg gttaaaattt gtacagtagc ccggcgccaa
    12901 aaacttcttt gatctgccat ttgttggaaa catccttatc gtaaacaaat tgtgccgtca
    12961 aattggcgct gacatattca ttcaccttca tcagaatcag gttttcccag ttgacatcaa
    13021 cttctttgat ttttttgtaa cttgagaaca tgtttacttt ggatgtaaaa tcaatgccgg
    13081 tcatcagagg cgtcttgtaa acactgttga taaaccaacc gaattctgta taaaccttgt
    13141 cacccttttt tacgccaaac gacccgaggt cagacagttg tttgtcgaga acaaaagtcg
    13201 ccttgcccgt aacgggagat atgagtgcag aaaatttatc gttgggacga tagtccgcac
    13261 caagtgcgat ggtgagcgct gccggcgaca taaatttgga aataggttta cgtaacgaat
    13321 cgggtacgct gtaatcatag ccattggcaa attgtgtacg aaaatccaat aatgccgaat
    13381 atttccacgg gcctacgaaa tcgcgattgt atttggacac ataaatgatc ttatcatccg
    13441 ctttacgtgt accggcatcg ccctgtttga cgataccgta acccaattcc aaagaatttt
    13501 cccagcgcgt tttttcaaaa tgataattag caaacaagct caaaagtgaa ttcagcgcaa
    13561 gcgagttatc gccgccgccg ccccagtcct tgagttgtac ctgactaaag ttgatactgg
    13621 ctgcgccgcc acggtgccat gttgtatcct gcgcatgagt catttgcgca aaaacgacca
    13681 ttaccaacgc aaagagtgta aatttagacg tcataggcat cggcctcctg tgggataggt
    13741 ttttattgtg gttcggatca aattcgtcgt tttacggacc aaaatctaat tgtttttact
    13801 tttgaccttc acggatttca tgcatatagc gatgcaacga ttccgtgttt tcagccggtt
    13861 gagattttat caattcgccg gctgccgcca gggcttcgtc aagattgccg aaataataat
    13921 cttcgcccat ggtttgcatc aggccggatt gctcgacagc gatgagcggc tgcgtatgta
    13981 tctcggcgat caagaaataa actttatctt ttcggcaatg attcatcatt tcacgtaacg
    14041 catgaagccc tcccgcgtca acatagggta cggcacgcat gcgaagaatc aatacgcgtg
    14101 tgtgacgtct ggcaaaatca aagtgttcct gaaatttata aaccgagccg aaaaagaacg
    14161 ggccgttgat ttcaaaaacc tgcacaccct ggggtatgcg tttgtccttg atggcttgtg
    14221 tgtcgggggg ttcgtcatga tcatccagat ccgacttgat cacctgaatg tgtgtaacgt
    14281 cggccatctg cttcataaat aaaaatgcgg cgaggatcat cccgatttcg atggccacgg
    14341 taagatcaac caatacggtc agcacaaacg tagtgagcaa gacgatcaca tccgttcgag
    14401 gaccgcgaat aatggccata aacgccgtat gctcactcat attatacgca acgtaaatca
    14461 gaatggcggc cagacaaggc aaagggataa gagccgccca ttttccaaat atcaacaaga
    14521 tcatcagtaa gatcagcgcg tggataatac cggcgatcgg cgtccggcct ccgttgcgga
    14581 tattggttgc cgtgcgtgcg atggcccccg tcgcagggat tcctccgaaa aaaggtgtaa
    14641 caatgttggc gaggccttgc gcgatgagtt ccatattgga gcgatgtcgg tcaccgatca
    14701 taccgtcggc aacaacagcg cagagcagtg attcgatcgc gcccagtaag gcgatcgtcg
    14761 tcgcgggcat aatgagggct gtgatcgtag accattgaat attgggaaaa ataggcgctg
    14821 gtaatgaaga cggaatttca ccaaagcggg tgtgaatcgt atctacgggc aattgaaaaa
    14881 aataaacgac acacgtcgaa atgatcaaag ccgccaatga gcccggaatt ttttgtgaaa
    14941 agcgtgacca aaaaatgata attagtaggg tagcgatgcc gataccaagc gcataaaaat
    15001 tgatagagga tccatgttct atatacgatg cccatttctc tacaaaatca ccgggtactt
    15061 tgtctatcgt taagccaaaa aaatctcgaa tttgtgttga gaagatgata acggcgatac
    15121 cggatgtaaa accggtgatg atcggatgag gaatatattt gatcaggccg ccaaatttag
    15181 cgagccccat aagaatcaat atcacgccgg ccatgatcgt ggcgataatc aaaccgtcaa
    15241 tgccgtattt ttctacaata ccatatacga tcacgacgaa tgccccggtc gggccgccga
    15301 tctgaacttt gctgccgctc aaaaatgaaa tcaaaaaacc ggcgatgatc gccgtgtata
    15361 aaccgcgttc cggtgaaacg cccgatgcga tagcgaaagc tatggcgagc ggcaaggcca
    15421 ccacgccgac tatagtgcct gcgctcagat cggcaataaa gcgttcgcgg ttatagtctt
    15481 tcagtgtatc taataattta gggcgtaaca taaaacctgc gcatgtgttt ttagtaatca
    15541 ataaccaatt gtaaacgtat catggattcg tgatcgcaca cgtaaccgaa aattttacgt
    15601 tgtacggcat caatgtcaat cacgccaaaa ttcagtccgc cataaaaatc accgacgcgg
    15661 tgtgaattag gttcgatcgg cagtaaacta taaagcgtgt aatttttggt gtgtgtcaaa
    15721 ccgctggacg taagctccgt cagtgtcgag cggccgttat ttcggccgtt ttcgatttga
    15781 gatatttcgg catagtgtcg gtcaccgctc acaatgagta cggtgccagg caccgttttg
    15841 agccaggata acattttgtc ccttgaagcc ggataatctt gccacacttc attgaacgcg
    15901 taattatcgt tggggataat ttgactggat gaagcgatca atgtgatatc cgtcgtatct
    15961 ccgaaaagtt caccgtacaa ccattgccat tgtatatcgc cgagtatatc cttgttggcg
    16021 cgcggatctt cacgaaatga acgttcatca agaagtatca actttatgcg ggtattgagt
    16081 ttgtcgtagc gatacgatgt ataaatacct tcttgcaagc gccgggggct ttcagcgggt
    16141 tcatccaaaa aatccatcaa tagacgttgg gtttcttttt tgtgttcata ttcgctgccg
    16201 ccgtcattga caccgtaatc gtgatcgtcc caaattccga tcaccggtac ggtttgtcgt
    16261 aacgcggcgt agcccggatg atttttttgg aaattgtaca tccatcgaat ggtgtcaatt
    16321 ccgccgcgat caccgtaaac gatgtcgccg agccacatcc aaagatcggg ttgggcagcc
    16381 ctgatcgggt tccatagcgg ctgggggaga aatgttttat tgcaagaacc gaaagcgata
    16441 cgaaatgaat ccggagtttt gccgggcaaa gggcctatcg gtgacggtgg tgtacaagca
    16501 gaaagtaaaa tgaaaaaaaa gaggacagcg gtggatttaa aaaaagttaa agcgttccat
    16561 cgttttactt ttttcacgaa gggtcaacct ttgtagttag gattcatcca agccatcggt
    16621 atatccgatt ccaaaccacc atccacactg cgaatacgat tttcaaaacg accttccgtc
    16681 acgaatccga gtttttcgta catgcgtatg gctttagaat tactctcgcg tgcaatgagt
    16741 tctacgcgca atatgttcgg catggttgtg cgaacggtgt ctaaaaaagt cgtaaagagc
    16801 tgttttccga tacccagacc ttgtgccttc gggtcaacgg cgatcgtgag ttcactcaaa
    16861 acatgatcaa atactttaat gcccggtctg taggcgtgta tttcaccaag gcattcgttc
    16921 tcattgattg cgatgatgga taaaccggtt gcggtgcttc gtgatagaaa atggtgaaca
    16981 taatcggaag tgacttcatg agcttcacga gcaagtcctc cgcgtatagc ggcaacccgg
    17041 ttataaagat tataaatggc cgtttcatcg ctcaacaaag aaggacgcag gaagaatgaa
    17101 ctcactagga agactccgaa tcgcggcgta tttttttgaa tcgcgcgttg tgttgttcgc
    17161 tgttatctac aaaaacaaat ttaaccggta ctttgtattt tgccatacgc tcgaggcaaa
    17221 atttgcggag gcgttccgta gcgagaagtg tgtcttccgg ttcatgcaaa gtgattttgg
    17281 cgtgcacgac gtgacccatg atcggatgag gtttcgcaaa aacgctgact tcgcgtacat
    17341 tgccggcttc cattaaaaca ttttcgactt cgacgggaaa aactttttga ccgccgacat
    17401 tgatcatttc cgatttgcgg ccgaggatgc gcatgtattc gccgcgcacc tcaacttgat
    17461 cgccggtaca catccatcct tcgttgtcaa aagggctggg cgcattgagg taaccgacca
    17521 tattggcttc agaccgaatc cacaaaatat tatcaacgat ttttacttca aaacctgcgc
    17581 ctccgatttt gacccacacg gaatcatcac tttcggattg ggagcgcaac acgccgagct
    17641 cggaaagccc gtaagtttgt ttaatctgcg cgttaggaaa aatatcgcgg actttcgaaa
    17701 gagttgcggc attcatgact tccgtaccat aagtaatcat tttgaccgaa gacaaatcgt
    17761 attgcccgta cacacgggat gccaaaagaa gattgataaa tgtcggtgtc gtgggcaaga
    17821 gtgtcgcacg tgaagtttgt atgatgcggc acacggtgtc aggattgcga tccgaaagac
    17881 aaacggccgt acctccgtaa gcaaacgatg ataaaaaagt gttaaatccc ccaaagtgat
    17941 ccatcatcaa aaaaagaagt gtacgccatc ccggtctttt ttcgacaaat ttatgcatga
    18001 cacgttcgca atcctgcaaa atacccttag gtttgccggt tgatccgctt gaaaaaacga
    18061 tcagccccgg tacatcgcgc tgcataaaat cctcaatcaa cgcgttgcgc ggaacggcat
    18121 tgtaccgttc caatgtccat gtatcgtccg gagcaaaacg gaacatacat tcgacaccgg
    18181 caatgttttt gaattcttcg atttcgtgtt ggatcgcata cgtaaagggc accatcacag
    18241 ctttgacttt catcaacgca aaaatgagcg ccgccgtctg cggtgaataa tcacctaaaa
    18301 aagcacaaac agtgccgcgg ccgaccccat tgtctttcaa aacaggttcc cacgcggcta
    18361 tagcggtgtt taattccgca taagaaaaaa aacgatcgtt ccagtacagc gcttccttgt
    18421 cgggtgcttc tgtgagtcgt cggagtatgg tttgaatgga ctgcatacga ttacgaaaaa
    18481 gtaactccca gttcttgtag tgtaactttg atgagcggca aactcagtat ctcgacgctt
    18541 tgttcggggg taaattctac gccaaattct tcttcgagac cgagtatgag gttcatgtgc
    18601 cgtaaactgt cccagttttc aacttcatcc tgacgtatag tgtcgtgcag cgatgagggg
    18661 tctacttcaa aaatccgtgc cataactttt tttatgtcat gggtcgtctt agccatatta
    18721 actcctgtaa gaaataaacg gaatgggtgt cgtatccagt gccgacgcgt ctatcgtata
    18781 tttcacactt cgatccgctt gtggcgtcgg tcggaagccg aagctgtccc aaaatttttc
    18841 agtttgacca ttctttttag tcggtatgta gtacgcttgc catgtgcgta tattccagcg
    18901 cggcgtgagt gccgccatga cgtgccgaac aaatgccgtt tctaatttgc gcccgagtat
    18961 gcggcaactg agtaaaaaag aatccacctc ggccgtatcg ccgttacgtg tcacgatgat
    19021 aaccgccgtc aaaccgaaat caccgaattt atcgccggca cgcatagtcc ataccgaagc
    19081 atccgggttt tctgcaaaag ctgtgatttg tgcttcggaa taacgccgtg tggtaacatt
    19141 gaattgattg gttttttgcg tcagttgtgc cacgcgcggt atctcatcgg ttggggcttc
    19201 gtggatagtg gcatacaact gaagggaggc taaataatca tcaatagatg tatagcgtgt
    19261 ttcttcgacg cgccgcatgg attcttcgtg catcatgcgt gtgcgatttt tgtcttctga
    19321 gcttacctgc aacgcatcaa aaaaaccgga tcgtatcaca aacggcggat aggtgtacaa
    19381 tttttgagga acctgaagta cggtgacatc cggtatcatt gttcggacta gctcgcattc
    19441 ggcggggctg tcatctacaa aaacaaaact gtcaaggccg atgttcagct cctctgcgag
    19501 ggagcgaata ttcgttgctt tatcagtcca gttgatacgc caagccgcaa tatgttcgcg
    19561 ccgaagcgcc atatccggat gatgatcaaa aacttcccac acgtcggcct cgttgttttt
    19621 actgcagagc gtaagaagaa taccacgctc atacaaagcg agtgcattaa gctggaagtc
    19681 gcgatacaca cgtcctggaa agctgtcgcg gtccagttta atactcgaca cgccgtcttc
    19741 gccgataatc ccaccccaaa gggtgttgtc gcaatcaaga acgaggcatt ttttggtttt
    19801 accttttaat ccgcggctga gtttggcgat atcgaaggca tacgcagaca taaaatcttt
    19861 tttaaaaggc gccttggacg tgtaccaaaa acgataatcc atagccgctt cttcacccag
    19921 acgaataaga tagcgctccc agtccatcag atagagtcgc gtcggatcgc gacgcacgcg
    19981 ttcacggatc tttcggttta ttcgttgtac ttcttcaaaa cgttccgcgg cgtccggtga
    20041 tcggtacatg ccccattcgg aatcccaagg cggcacgaat gtgttaacgg caagcaaggc
    20101 ttgtgtgttt tgtaaagccg tatccagcaa cgattcgatt tccgatatga cgcggtctgc
    20161 agtccatccg aatgtacgca ggttggtatc caaagtctca agtacgagac cgagcacgac
    20221 gatgtccgga tggcgctgat acaacgagga atttttatct aacaattcct gccggacatt
    20281 gtcatagttc ccgaaactga tttcaggacg tatgccggat tggtagtgat aaagcttgag
    20341 aaaaggttca atgccttcga tcgtaaaatt acgcaaaata tgaatgcgta cggattgctc
    20401 cggttttttt tctaaatggt cgagatgatt taccagagca atggtttccg aaagcgtatc
    20461 gggaagcgtg atgtctgtaa ggcgttcggg gatcatcata acatcgtttg accgccattg
    20521 acgcggatcg tctcgccggt gatataatct gccgcatcgg acgccagata agcgatagtg
    20581 ccggcgacgt ctttggattg ggcaagccgt ttcatcggtg ctttcatggc attcaatagt
    20641 tttacttttt caggtacgtc ggctaccagt tccgtatcgg tcatgccggg cgaaacgaga
    20701 tttactgtaa tattttttga cgccatttct accgccaacg ctttggcaaa tccgttgagc
    20761 gcatatttgg cagtaacata acccatgagt tcagttggcg gtgtactttc tgtgtacatc
    20821 gtagttagca aaatgatctt gccgtacttt tgcttttcca tcagcgggat gatacctttg
    20881 cagaggtaaa atgatgcccg cacattgatg tcccactgtt gggagatatc atcccatgcg
    20941 accgaagccc acttgatggc cggtattttc accgtagccg cattgaccaa aacagttacg
    21001 gtcccgagtt ttcgtgttac atcttcggcc aaatggtgaa tatcttccgg tttgaaaata
    21061 tctgctttaa acgcatgtgc tgcgcgacct tcgtttttaa cagaaaccac aagatcgaaa
    21121 gctctttttt cgttggaaaa ataatgtaca gcgacatcaa aaccttgttt tgcaagcata
    21181 cgacatgtgg cttgaccgat acctccggta gccccgatga tcagcgccac tttctttctc
    21241 acggttgttt cttcagctac ggaaggtttt tcctgagcga tcactttgac tttggctttc
    21301 ccggtggtga ctttttgttt gtgctgattg atgatatccg tttgcaattc gatgatttgt
    21361 tcggcatcta ttttacgcag gacttcagcg cgaacggtga tctcgtctcc gatacggacc
    21421 ggcagtagaa attccaaact ttgagaaaac cacaatgcac cgtcacccgg aagtttggtg
    21481 ccgattactg ttgaaataaa ggatgctccg agcatgccgt gtacgaccgg ttttttaaat
    21541 gaggtgcggc cggcgaagtt ttcatccaca tgcattttgt tatcatcgcc ggtcagagcg
    21601 acaaattttt caatgtcgct tgccgtgatc gtgtggcgaa tttcagcggt gtcgccgatt
    21661 tgtatctgtg tatagtcggg catgtttttt tagggtaagt taatcaaaat aaatcagagc
    21721 gcacgttttt tattagtacc agcgcagaac tgtcgcgccg aaaataaatc cggcaccgac
    21781 gccggaaatc acgaccaatt gatcgcgttg caatttgccg gcaagtacgg catcgcaaag
    21841 cgcgatagcc atcgaggctt cgccggtatt gcctatggtt tccacattgg tctgcgtttt
    21901 atgcatggga tgcttcatct tggccatgag aaattcgatt aaccgaagat tggcctgatg
    21961 aaaaataaaa aaatcgacgg catcggtttt tttggaaatt ttttcaagca cacgcatgat
    22021 gacgcggggt tgaaattgca ccacctgttt ccatacttcc aggccgttta attcaaaata
    22081 cggaagtaca tcgtttacat tctccgttcg cagtgggtag cttgatccgc cgccgcgaac
    22141 gcgcacggca tcgtacactt tgccgttggc cataatatcc gaggcgagta caccatatcc
    22201 ttccggtaca cgcgcaagta ttgctgcacc ggcgccgtcg ctaaaataaa tagccgaatt
    22261 ggggtctttc cagttgataa aacgggattg cagcgcagta ccgatcacga gagcataggc
    22321 tatcgaagaa tccagggtca tgcgatcggc cgcattggcc aagccgattt ggaagccggt
    22381 gcaattggcc ataacatcgt aagcgcccgc atttttagcg ccgagttgct cttgcacgcg
    22441 gcatgccatc gccggacaca gataatctcc ggtgaaggtc gcacaaataa tcaggccgat
    22501 ttgatcggca gtgatacccg ccataaccat ggcttgtcgg gccgcttcga cggacatgcc
    22561 cgatgcggtt tcgtgcgctt cgacgacaaa tcgtttggta acgccgattt tagaaacaat
    22621 atcggcggcc gatactccgg tccacgcttc gacctgctca ttggtgattt ctttggaagg
    22681 aatatatttt cctacgccgg taatgcctat acccatgaat attttatcgt gttacgttaa
    22741 gcaaatgtat caaaatgatc atgtacgccg ggttcatttt ttgccgatgc ccaccattcg
    22801 cgcgcttcac cgatcgtcca tggttttccg tggcctgcaa aaatacgtgc cgtatcgtga
    22861 tgttcggaca gcagtttatc tacggactgt ttcagcatag tcatgtcgct gcccggtaaa
    22921 tccgctctcc cgacatgttt gtgcatcagt gtatcgcccg taaaaagggc attgcccagt
    22981 acgaacgccg cgccgccttt ggtgtggccc ggcacatgca gtgcgcgtag ggcgagagca
    23041 cccagttcaa atacgtgcgg cgtctcaaaa aaattgaccg gctgcggcat cggaatgggt
    23101 tgacggccga aacgaatcgc atacatcggt gcatgccgta ttaatttatg gtcatcttta
    23161 tgcaaatagg ccggaatgga aaacatacga caaatcggag ttacagcgcc cacatgatca
    23221 tgatgtgcat gggtcagcaa aacccaagtc agtccgcggt ccccgttttg ggaaatcaga
    23281 tccgctaaac gatcggcatc ataaccggga tcaatcaacg cttgttcgcc ggatgcactt
    23341 gtgataagat aacaattttc tttccacagc ggatccgtca cgacttccgt gattttaaaa
    23401 ccgtctaata tatattcgta aatgccttcc atgccgtatt cgcgctttcg tccaaggtca
    23461 gtccacaaga cccatataag tgacttggcc tgtgatgcag gcgctttcgg gcgcggcata
    23521 aaatgagatc acattggcga tttcgtctgt ggtgataggg cgctgtatgg tctgctgggc
    23581 taacatattt ttttcccatt ccgggccaaa cgatttagcc gcatccgtcg gtgacatcga
    23641 aggcgcgacg acgttgcaag tgattccgag cggcgccatt tcacgggcaa gcactttggt
    23701 catttgttcg atagcgcttt tggtggccga atataccgac gtgccgggcg catgaaggcg
    23761 cgtcatgata gaggaaatcg taatgatacg accttgtttc tgtagcaaca tggtcttggc
    23821 tgtctcacgg catacgtagt acgtgccggc cacattggtc ttgagatagg cttcgagtac
    23881 atcgccgggc gtgaccgtca tctggagcgc cgattcgacc aaaccggcat tgcaaaccaa
    23941 aatatcaata cgaccggctt ctttttttac cgtgcgaatc cataaccgca cggcggattc
    24001 attactcaca tccaaagccg tgtgatgata agcggcatgt gtgatcgtcg cctcgcttcg
    24061 gctgcatccg aaaacgcggt atcctttatt tagaaaatac tctgctaatc ctttgccgat
    24121 acctttgctg gtaccggtga tgatcataac cggcgtagcc atgatgttat cctttttgaa
    24181 tcaagccgac gatgtagccg gctatggttt cggctgacag aaacgggtta ttaatattaa
    24241 aaccttcgat ttcatctaac acaagaaaga tgtctttgcc ggaagccgtc gcgatgcgtt
    24301 cttccaaatc ggtaaacaat gtaacaaaag ccatcgaatc cagcggggat ccattgccga
    24361 gaattacaag atccggtgtt acctgcactt gcgaagcgat caatccgctg cgcgtaagac
    24421 tgtcaaacga ttcctggata acgtttaaaa tctgttcggt attcattcac actccaatat
    24481 tgctaaactg aaaaacggaa acctatcata cgtattttga gcggcttttt caatgaacga
    24541 ttatagtcat tgaagatgcc tagtgatata atcacgataa ggaatgatgg ttgccccgtc
    24601 ggcaaggatg gtatttattt tatcaagcat cgtgccacgg ttttgataca tcacccagtt
    24661 ataaaaccgg ttgtctttta agcccggatg cataaagcgc tcctgctcgg tgtcaatatc
    24721 ataaggatga aaatagctga gcaccgggcg cgattgattc cagtgatgac gaaaggcgcg
    24781 cgccgtcagc cacaaaggca atgtgcgaaa gtacaaacca cccgcacatg ggacggaaat
    24841 aaaacgcgaa ggaaaaagag tgattggtaa ttcccagatg cgatcatgca ctcgacgaaa
    24901 ggtcgctcca aattccggcc aaccgtacaa ggggttggtc gctggcagca ctgaactgga
    24961 atacgtaaaa ccttgctcga tgagaatttc ataagcccat ggtgtttttt tggtcaacga
    25021 aaaaataggc gcacgaaatc cgttcagagt ctccacgccc gctttgtaaa gtccgtccat
    25081 gttgcgaagc aggtcatcgc gaaatgtttc cggtgtttgt tggtcaagag gtatgtgccg
    25141 atgcgtatgg caagcaattt catgccctgc ggaatagatt tcccggatta gggacgggta
    25201 ggcttctgcg acatcgccca cgacgaaaaa agtgcacgtc atgcgatgtt gggccaaaaa
    25261 tttcagataa cgctccgtca tggccgggac gcgttcacgg tactttttgc cgtcgctcat
    25321 ccaaaagcga acgtcttcga gatcaatact aaaaagaaaa tatttttcca aaatggcttc
    25381 gcagtttttt taataaatcg gcctaagata ggagaattgg tttagcgaat caaccggaga
    25441 ttacggtact ctgcttatag aggatgttta taaaaaaaat taaatattgt tgtcattcga
    25501 tagtctctgg tattaaattg taaccacatt tcttaaacac atcctatgct gccttttttt
    25561 atcagacata ccctcttttg gtgggttttc gtaagccatc tttttgcgca gcaatattat
    25621 ataaaaaact attccacgtc cgacgggtta ccaagtaata ctatttttga agtttttgtt
    25681 gattgcgatt acgcgatgtg gattggcacc gtggacgggt tgagttatta cgatggacaa
    25741 acctttcaga attttaataa aactcaggga ttgatcaatc accgtgtatg gtctgtcgct
    25801 caagactcat ccggcaacat ttgggttggc acacgtacag gcatcaatcg tttccacaag
    25861 acccgtagcg gatggcaaat acgcgcatgg ttaacgcagt tgtctgttcg tcacattcat
    25921 atcgaaaccg atcaatccat gatattgagt acggaaaacg gagcatacaa gtttacatgt
    25981 ttcaccgatg attcgagttc attggtgccg attgagggtt tacaaggaaa aatggtttat
    26041 caaacattgc gtgatccgga taacagattg tggatagcaa cggatcaagg cgtgtggata
    26101 caaaaaggaa cggtattaga aaatatctcg accccttttc cgattgtgcg gggtttgttc
    26161 ttacgtgatt catcacactt gtatgcttta cattttaggg gtggtataag tttgacggat
    26221 cctcgatcgg attccccttc gtttgtttca caagatttac caaaaggatt tacgaaacgc
    26281 agtttttacc gcatgatcgt tgatcccaca ggtaattttt gggcggctac cgatttgggt
    26341 ataacacggc ttcggttttc agatacacgc gtcgtttcga tggaagttat tgacgaaacc
    26401 aaagggctag ccaataatct gactataccg ttagttagcg atcatgaggg gcatatttgg
    26461 gtaggcactc acaatggtct ttcgcgtttt gcgggtttac gtttttcata tttcattcct
    26521 cataataatc ccggaattca agtctataaa tccattgccg catcagggac ggatatttat
    26581 atgctgacca gcgccggtct tttctatgtc gatgcgcaac accccgcagt gccattaccg
    26641 ttgtatggta atacaccgat ccaagagggc gtgtcgcttg gaaaaaacag attggccttg
    26701 gtcaatgaca gcgaaatagc aatttttttt gttggcggaa aaactttcag aaaacaaaaa
    26761 aaaatatcgg ttgaaggtca aagtattgca tcgtttttgt ttctcgacga acaagatgta
    26821 atgatcggca catccgccgg gctttatcgt catcggcatg gccgagtaac acgtgtcaat
    26881 gcttcgacat tcacagatca aatgatggtt tatactctta tgcgcgatga taaaggggcg
    26941 atatgggttg gtacatcaca tggcgcctat gtgttgacag cggatcagga ttcactacga
    27001 gtccaatcat ggtctttacc cggtgttatg ccatttcaag ctgttggtca gatttaccgt
    27061 gatgcgtcgg gcatgatgtg gttttgtacc gaaggcgcgg ggcttgcacg ctgggatggg
    27121 caaaatgtac gtgtttatac taccgataac ggtatctcaa ataatattgt gtactctgtt
    27181 ttcgaaccgg atacgacgac tctctgggtt tgtaccgccg gcggtattga tgttatgaac
    27241 cgaaaaaatg attcatggat gcattgggac agccattctg gattttttcg gaaagggagt
    27301 acgccatact cgatcatgaa aagttccgat tccgtgtatt ggatcagcac gagtcgtggg
    27361 ttggtgcgta cagatttcag cgaaccgttt gcgtccggaa actctgttcc ggtgctggtg
    27421 gatcagattc ttgtccaagg taaaccccaa cgacacagtg ggattttgcg tatcgagccg
    27481 ggagttcagc aaatccagtt tcgcatcaag gcgatatctt accgttacga gaatgatgtg
    27541 cggtacagtt tttttatgga gggttttgac gaagcttggt cggagccttc ctcgcaaggc
    27601 gtgatacaat atgcgggctt atcgccgggg atatataggt tgaaaagcaa agccatcaca
    27661 cccggcggac aataccgcgg accggatgtg gttttggaag tattgacccc gttttggaaa
    27721 gagacgtggt ttgtagcgat atcgtgtttg gtgcttatcg ccgtgacggc cggcgcctac
    27781 cggtggcgaa cctatcattt atggaaaaaa gcgcgccagc tggaaatgct ggtgcgcgat
    27841 cgcaccaaag atttggtgcg taaaagccga gacgtgatca aacaaaaaaa agaacttgaa
    27901 aaaacattac aaaccctacg cactaccgaa gcgcagctca tccaagcgga aaaactgtct
    27961 gctctgggtg aactcacagc gggtgtggcg catgaaatta ataatccgat tgcctttatt
    28021 tacggtaatt taccgttgct ggaaaaagat cttcgccgtt taacggagct ggcgcaaaaa
    28081 gctaaaaccg gaccactgga cgacaaagag gcgctggaaa tgactcaaat tgaaaatgat
    28141 attcattcgg cgattgtatc aacgcggcac ggagtacagc gggtacaggt tatcgtcaaa
    28201 acgttaaaga agtttgttcg actggatgaa gcgaaagagg attttaaatc cgtggaagaa
    28261 tgtctggata gtgcggccga tgctataatt atccagtatc caaaggttca gatcaaaaag
    28321 gattatcggg agacgccaca aattttgtgt tatccgtccg aattgcagca ggcgttttat
    28381 catgtcatgc tcaatgcgat acaatcgcaa gcaaccgatg cacgggtgga cttttcaaca
    28441 agcaccgacg ggaaatatgt gtatgtggga attcgcgact atggcacggg catccctgag
    28501 gctataagcc aaaaaatatt cgatcctttt tttacaacca aagacgtagg gcgcggaaaa
    28561 ggattgggat tgtctatgac ttacgggatc atggaaaaac acgagggtgg gatacggttt
    28621 gaaaactgtc aagatcaggg tgcgaaattt atactgatgt tgcctgtaga gcgtaggtct
    28681 tcatcgccgt cggtgttatc gtagtcgcat agaagaattg caggaaagaa ataaatagta
    28741 cttaagaggt tcgataaaaa acgaatgttc ttatatcaca tgaatcggga ataatagact
    28801 aaatgtacta cccatgcctt catggctttt tactaccaaa cgcccaccgt tgatttctgt
    28861 aagatcacgc gcaatggcaa gaccgagacc ggcaccttgt tgttccattt cagcgcgatt
    28921 gacttgatag aatttgtgaa aaatacgatc taactgctcc tccggaatgc ctatgcccgt
    28981 gtcttcgata tccaaacgta catagtcgtg gtctttgccg ctgcgaatac gaatttctcc
    29041 gccgggcgga gtgaatttat aggcattgct aaataagcgc tccactacat cggccacttg
    29101 atctacatgg agttgtaacc ccggcaaatg ccgttctagc tgagattgga ttcttagttg
    29161 tttgatctcc gcccgctttt tgaactgcgg tacgagatgc tcaacgatga agttgatatc
    29221 agaatcttgt ttggcttctt tgtattcaga ttttgcatca ccggctgcaa tgcgtacggt
    29281 gatcaggaaa tctttgagca gggcatctag acgttcgccg ctttttttga tgcgaagaag
    29341 aagttcttgc agctcatggg gtttaaactc catggtttca tcaagcaaaa gattggccgt
    29401 aagcttgatg ataaaaagcg gagtattgac ttcgtgtgtg atcgtcgaaa ggatttctcg
    29461 tttcaattga tcgatttgcg tcagcatttc cgcttcggtg ctggagcgaa tctcttctac
    29521 gcgtttgagt ttgccgcgaa tagaggcaac cagatcttct tgttcgatcg gtttggttaa
    29581 aaaatcatcc gaaccgattt ctttaccata gcgacgtttg tcggcgtcgg aatgtgccgt
    29641 gagaaaaata aatggaatgc tgcgaagatc gggtatggct tttacgttgt tatataattc
    29701 gaaaccgtcc atgtacggca tcgaaatatc gctgatgatg agatcgggct tatacgtttt
    29761 tagtttttcc atcgcggcaa aaccattggg cgcaagtgtc acgtcaaaat tcatccggct
    29821 cagataaatc tgaattaatt gcctgttttg gaagtcgtct tcgacgacca atattttggc
    29881 gtgagtgctc atggatttat ttgacgcgca gtccgccggg catgacggcc tccggggata
    29941 atattttgag tgtggtaccg tcgctgaccg tcaggatcat cccgttggat tcaacgccgc
    30001 gtattttcgc cggtttgaga ttggcgacga tgatcacgtc ttttccaatc aattcttccg
    30061 gcttataata ctccgccaaa ccgctcacaa cctgacgttt ttcaaaacca agatcgagct
    30121 ggagtttgag taatttggtt gtgccttcga ttttttcggc atgcagcacc ttggcggaac
    30181 gcatatcgat tttaccgaat tcatcaatac ttatttgcgc tgcagtgacc gcttcatttt
    30241 cggatttcgg tttttcaacc ggtttggact gcgcactttc tgtcgttttt tgttcgatca
    30301 ttgagttcaa atgatctatc tccttttgta tcacatcatc ttcgatttta gggaaaacaa
    30361 tttcaacttt gccgagcacg tggccggtgt ccattcgcga ggtgccgatg gtattccaat
    30421 cctgttcttt gactaggccc gacaaaccga gcatttgcca tactttttca gaggtaaatg
    30481 gcaagaaagg atgcatgatc acggctaatg cgcgtacgac ttgtacgctc acccacaaaa
    30541 tcgttgcgcc gcgttccggt tcggttttga tttttttcca gggctccgta tcgttaaagt
    30601 atttattggc ggcgcgtgcc aaatccataa aacgttttaa cgcatcgcgg aaacgaaatt
    30661 cttcaataaa tttgccgact tcggtttgcg cggccaggat ttcggaaagc atttgttcat
    30721 ccatagccgt aagcgtgccg cgcgacggga ctttgttgtg gtagtttcga atcgtaaagg
    30781 acaaactgcg attgatgaag tttccgagaa tatctgctag ttcgctgtta tttttaatct
    30841 gaaaatcttt ccatgaaaaa tcggaatctt tgttttccgg aagattggat gtcaacgtgt
    30901 aacgcaaaag atccggagga aacttttgaa gataatcttt tacccaaacc gcataattac
    30961 gactggtcga aagcttgttg ccttccagat tgagaaattc gcacgccggt acatcggaaa
    31021 ctaaaaccaa acggcccgat gtcttacttt gattgtaggc catcgtgatg atagggaaca
    31081 tgatcgcatg aaatacgata ttatctttgc cgatgaagtg cacgagttgg gtatcgtcct
    31141 tttgccaata atcacgccat ttttcaggtt ggcctacttt ttttgcccat tctttggtcg
    31201 aagaaatata acccaataca gcttcaaacc agacgtagat tacttttcta tcggaacctt
    31261 cgatggcatt attcttacgg tcttttttgg gaaccggtac accccaatca atgtcgcgtg
    31321 taatggcacg gtcgccaagt ttctgtgcaa accatccttt acagtagttg atgacattgt
    31381 ctttccagtg agacttggta gatatccatt tttccagttc ggattgaaat tcgcccatcg
    31441 gcaaataaaa atgggtggtc tctttggcga tcggagtagt gccggtgact ttggagcgcg
    31501 gttggatcag atccatttga ttcaaatacg taccacattt ttcacattga tccccgcgag
    31561 catccgtgtt ttgacataca gggcaggttc cttcgacata ccgatccgag aggaacattt
    31621 tggcttgttc atcataccac tgcatctcgg gtttgcgttt gagcagattt ttttcaagca
    31681 agtcgagaaa aatttcctgc gccgtatcgt gatgaaccgg attggatgtg cggtcgtaat
    31741 ggtcgaagct gatgcccata cgagaaaact cgtcctgaat catcgcatga tagcgatcta
    31801 caagggcttg aggcgtaacc ccttcttttt ccgcttgtat cgtgatgggc acgccgtgtt
    31861 cgtcgctccc gcaaatgtaa acgatatcgc gacgcatgag gcgttggtag cgaacatata
    31921 tatcagccgg gagatatgcg ccggtaaggc gtccgatgtg actgggacca ttggcataag
    31981 gtaacgcact cgtcacaaga atgcgtttaa acgatgtgtc ggtaggcaaa gctaactccg
    32041 taggaaaaac aaactgtctg aatctaaaaa attatttggt tacgtaaacg acttcttgtt
    32101 gtgcgcaggt gaggcgcaag tcgctgtatt tttctttgag gcgcagttcg atacgatcca
    32161 gttcacggtc ggtgcgcatc ggttcgtgat gcgtgagtaa taaatgttta cactcgatct
    32221 gctgcgcatt gcggcaagcc atatcaaatg tactgtgccc ccaaccgaca cgttgcgcgt
    32281 attcttcatc caggtacatg gcgtcgtgaa tgagcatatc cgcgcctttg gcccaggtaa
    32341 taaattcacg attgacagaa ttatcaatag cttcgtgatc gctggcgtac actacactgt
    32401 ggccgccgtc gctgattttg tatgcgaagc aggggtcggt gtggttgagc gcacggcatg
    32461 aaatctgaaa tccgttgatc tcgatatctt gcgtaagctg gtgaaattcg agcttggaat
    32521 tgagttgctg ccatccgttg aaagggccgt aggaaccgtc aaagtagata tccatcactt
    32581 ctttgagttt gtcattcgat tgtggcgaat aaaaatgatt gatacttttg gggtggtgca
    32641 tagcgccgaa atagttgaga ccctgaatgt gatcccagtg aaagtgggaa atcaaaaggt
    32701 gaaattcacc catcttgttt ttaagatgat cggaataaaa tcctatacca aaacccgcat
    32761 caataataac actgcgctct ccgctggaaa taaccacgca tgccgtatgg cttccgtatt
    32821 tgagcgaatc ttcgtgtgtc gcgctgatcg agccgcgcgt accgtaaaac gtgacttcta
    32881 aacccatact tccgttccct cccgcaaggc caccacttcg atagcgcctg cttttccgat
    32941 ggattttaca tagtcattgg ccgaagtgat tttttgctct acaaaagtgt ccgtatcttc
    33001 cggggcatgg tgcccgagta tgatcttttt cacgccggct ttgactgcga gatcgacagc
    33061 catttcgggt gtcgaatgcc cccagttttc tttaccttta attccttcct tggtaaagtg
    33121 cgtgtcgtgc agcatgatat cggctccggt gcaaaagcga accatatttt cgtagaattc
    33181 tttttctgtt ttagtgaagt cttctttgtt ccatgctttc atcaaaacgc cgagatgatt
    33241 gttttcgatt ggcgcatgat cggtgatgat caaaaccgat tttcctccgt gttcgatccg
    33301 atagccgagc gttaatccct ggtgattaag ttgcacggtt ttgacggtgg cgttttcaag
    33361 cgggatcgtc tgatcgggat gtaattcgtg gaaagtgatc ttatcgcgta ccagtgaaaa
    33421 aggaatcggt atcaaacctt tggcatgctg aagtttgaaa agttcttcga tggtgtattc
    33481 ttcagagcgc gggccggata tggaaatttt ggtgttttta ttaaataacg gtgcgaaaag
    33541 cgggaatgcg agaatgtgat cccagtgcgt gtgcgtgata aaaaagtgtg catgaaatcc
    33601 ttcagggtgc tgttcggaaa tcgtaacccc ttcacgatag atgcctgtgc cgccgtcaat
    33661 atagattggc tgcataccgt caatcgtgat tttgaaggct gttgtgtttc cgccgtagcg
    33721 aacccggtcc ggcgtcaata tcggacgcga accgcgtgtt ccaagcagtg taaacttcat
    33781 gcccaccgtg tactcccggc tttgttgatt atttataatc aaccacgaat actacacgtt
    33841 tgttacgcaa acggtaatat tcgtggtgtt atgtatctca tttcaaaaaa aacgatatta
    33901 tgctttcgga ccagcttttt taacgtcgtc cgaagcttta tcggcaaatt tttcaaaatt
    33961 tttgatgaat agatcggcca attttttggc ttgggcatcg taaccggctt tatctttcca
    34021 cgtgttttta ggaacgagga tttcagccgg tacatcaggg catgattccg gtacagccac
    34081 gccgaaaacc ggatcgttat tgtatttcac gttattgagt ttgccggaca gtgcagcatt
    34141 gatcaatgcg cgagtgtaac tcagcttcat acgtgagccg acaccgtatc cgccaccgct
    34201 ccagccggta ttgactaacc atacggtagc gttatgttcg ttgagttttt tacgcaggag
    34261 ttcggcatat acattgggat gcagcggcca gaaaggggcg ccgaagcagg ttgagaaggt
    34321 tggcgtcggt tctgtcacac cggcttccgt tccggcgact ttagccgtat aacccgataa
    34381 gaagtgatac atcgcttggg ccggtgtcag tttgctgatc ggaggaagta cgccatatgc
    34441 atcgcaaacg aggaaacaaa tatttttagg atgtccgccg atacccggaa tgatgcagtt
    34501 atcaatgtat tcgaccgggt aagtgacgcg cgtgttttca gcgatcgcgg cgctgtcata
    34561 gtccggcgta ccgtcttgtt tgagaacgac attttccagt acggaaccaa aacgaatagc
    34621 attccagatt tgaggttcgc cttctttgga gagtttgatg gtcttggcat aacatccgcc
    34681 ttcaaaattg aagacgcctt cgttggacca tccgtgctca tcatcaccga taagacggcg
    34741 gagcggatca gctgagagtg tggttttgcc ggtaccggac aaaccgaaga acaaagtcac
    34801 ttcgccttct ttgttgctgt tggccgagca gtgcatgggg aaaataccgc gctcgggcag
    34861 aatataattc attaccgtga aaatcgattt tttgatttcg ccggcataat gtgtaccgcc
    34921 gatgaggacg attttctttt caaaattgag catgatgaaa gtcttcgtgc cgagaccgtc
    34981 aaattccggt atggcgtgca taccgcaaac atctatcact gtccaatcgg catgatgcgt
    35041 ggccaattct gcttccgtag ggcgaatgaa aagagtttta gcgaaaagcg aatgccatgc
    35101 tttttccgat atcacgcgca cggacaaacg atttttagtg tcggcgccgg caaagccgtc
    35161 gaacacaaat acgtctttac cttgcagata agagcgcatg cggttaaaca tcacatcaaa
    35221 ctgagcttgc gaaacttttt tgttgttttc ccaccagatt ttgtctttgc tggtcggctc
    35281 ttcgacgacg tatttgtctt tgggggagcg gcctgtgtgc ggtgtcgtat tggtcacaag
    35341 tgcaccgttg gatgcaagct gaccttcgcc gcgtttgatt gcaagttcga ctaattgact
    35401 gaccgaaaga tttttgtgga ttgtaccggt attaacaata ccaaggtgag ctaattcatt
    35461 gttagtagtc attggaatat ccatctccta agtgattatc gagttccgct gtcagatttt
    35521 cggacgcgga aagttgtatt ggtcaaaaaa aggatcattt tccataaaat ctttgagcgg
    35581 attttgcatc gtgccttctt ccacgtgatc gtgggtattg catttaccgc tttggcaacc
    35641 gtcgccgccc gaagcgcaac cgctgccgca accatgtata tcgctcatgt caatgccgag
    35701 aatgtcggcg acatcctcca gcgtgagcgt ttcccattca ttatcatggt cttgtttgat
    35761 gtggactttg ttgccgatga tatcaagatg cttgatctca ccgcggcctt ttttggtttt
    35821 gatcggagaa ccgacacccg gaaatcggga taacgtatta acataaaaat cacgttcgta
    35881 tgccaagcaa cacttgagcc gcccgcacaa tcctgtcagc ttggaaggat tgagttgtaa
    35941 gccctgatct ttggcgtatt ccgtggtgat cggctggaat tcggtcaaat gccccgaaca
    36001 gcaaagctgt ttaccgcacg gaccgtaacc gtcaacgcgt ttggcttcat cgcgtacgcc
    36061 gatctgccgc aactcgatgc gcgttttaaa ttcgccggcc aaatctttga ctaaagaacg
    36121 gaaatccacg cgcttttcag cggtgaaaaa gaacacgatt tttttacgat caaactggta
    36181 ttccgcatcc accagtttca ttttaaggcc gtgttttatg attttttgct ttacgaccgc
    36241 tttggttttt tcttcttcgt tgcggttatc ggccaatttg ctgatttcat ccggattagc
    36301 aacccggatg aattggcgca attctttgtc tttggtgaga tcttcaggta tttgttcgtg
    36361 agtcagagcg accttaccca tatcaacgcc gttttcgcat tgcacgataa cgtggtcgcc
    36421 gactttgacg accagattgt tcttattgag aaagatctcc ttgcggttgg ccttaaactc
    36481 aacctgaatg ggattttgca taaaatatat aaatcaccgt tttaccggcg actatttccc
    36541 atattgttag gatttaccgg atgaagggtt gacgatggaa cgaagattca accctaaatt
    36601 cattaaaatt aggttaatat agatatttcg ccctataagg tcaaccgctt tttcgatttc
    36661 agacagagct tcttcgacac gaacgcgggg aaaagctttg atgaattttt caagtcgttc
    36721 ggttttatct tcattgatca aaaagcgatc tatgagcgac ttgtcttcac ccaattcgcg
    36781 catatacacg acatcctgaa accaagttat caacatcgtc aaaatgtccg tataaaaact
    36841 cttatccttt tcggcctctt cgttccactg ttcgatgatc gtctggatgg atgctgattt
    36901 attagacatc gctgccaata agaactccag catggcttca cgctttcgcg gaaagtctcc
    36961 ggagatcagt tcactggcct gatagtagtt accgttggcc agtcggctaa tgaatttagc
    37021 ctggttttca gctacatcgt agtgacgacg gataccgtct aacagttgat catccgtgag
    37081 gcggtgcagt ttgacaatct gacagcgcga tatgatcgtc ggtagcaggc tgtgaatatt
    37141 ggtcgtggtg agaaagtata acgtggatgc cggcggttcc tcaaggattt ttagaacggc
    37201 attggttgct tccgcgtgca tatcttcgat aaatgtcaga ataaaaattt tgagttcgcc
    37261 ttcaaacgaa cgcaaactga tcgctcggga tagccagcga atcgaattga cttccggatt
    37321 ttcggggtcg ccgataggaa tactgccatt ggcgatgcga acccgggcgt acaggtttcg
    37381 cgttttttct tccaataaac ggcgctgttc atcttcttcg gtggctcgca tcaccggaaa
    37441 ataatagtgc acatcgggat gctgcagggt cgccattttt ttacaattgg cgcattctcc
    37501 gcacggttcc catcgttcgc gatccggatg tttgcatagt ataaagcgtg ttgcttccag
    37561 tgccaattct tcggcaccca atccttcatc gccggcaatg agataggcgt gtgccatgcg
    37621 gtcgttggca aatgccgtga gtatctgttt tttagcggcc tcctggccga tgacttttga
    37681 aagcatgagc ggaatctatg tgagcttatc ggttgggctg attaaaacag atatgaattc
    37741 atcgaggaat tcatcgtcat gttgattgca acgcgataag caggtgttac attactatat
    37801 ttatcaatat atgcgcatcg tgacgcctta tcaagagtat gtcataactt gacaaagtct
    37861 gtccggcgca tgttactttt tttcctccgg ccgcatttta aaaatcagtg cgtggccgtt
    37921 ttgaatatat ttttgagcga ttttgcgaat atcttttgcc gataaacgac ggatcaattt
    37981 ttcctgtgct tcgggagtaa tgtttttgat ggaagcgttt tcgtaataag cgttttcgat
    38041 ccatcgtaac cagtaagcgt tctctttttg attccgctcc agatcacggc gcaatgcttc
    38101 acggattttg tttacatcgg attcggccgg accattttct gctaatttac gaatctcctc
    38161 gtgcact
//
LOCUS       DAOQNQ010000005        35898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_379_length_35898_cov_6.523868, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000005 DAOQNQ010000000
VERSION     DAOQNQ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 35898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35898
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_379_length_35898_cov_6.523868"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..737
                     /locus_tag="PK754_00750"
     CDS             135..737
                     /locus_tag="PK754_00750"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNB08063.1"
                     /translation="MIRAIFFDLGKVLVDFDWNTAAEHISRKSELKITEIIYRSTASD
                     VAMSFECGQLDSKEFLEKLKLSLRFRGTTDELHFLWTEIFTPIHNNIELLYRLKPTYT
                     LGLISNTNALHFDYIRNTYDFTKEFDRLILSYEVGAMKPARQIFEKAIAGMNLRSEEI
                     LFIDDIAKNLDGAKDLGWQTIHYTGHDELMKKLQTFMVII"
     gene            complement(797..2137)
                     /gene="gdhA"
                     /locus_tag="PK754_00755"
     CDS             complement(797..2137)
                     /gene="gdhA"
                     /locus_tag="PK754_00755"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNB08064.1"
                     /translation="MSDYVKSVLAQVKAKNPAEPEFHQAVQEVAESLEPVLDRHPEYR
                     TAKILERMVEPERVVMFRVPWQDDQGEIHINRGFRVQMNSAIGPYKGGLRFHPSVNLG
                     ILKFLAFEQVFKNSLTTLPMGGGKGGSDFDPKGKSDNEVMRFCQSFMTELYRHIGADL
                     DVPAGDIGVGGREIGFLFGQYKRLKNEFTGVLTGKGINWGGSLIRPEATGFGVTYFAQ
                     EMLRTKGKDIKGLTFAVSGFGNVAWGAALKISELGGKVVTISGPDGFVYDKDGIQGEK
                     INYMLKMRASGKDRVKDYADEFKVEYHAGKRPWSVKVDVAVPCATQNELGVEDAKELV
                     KNGCICVCEGANMPSTLDAYEVFKQAGILFSPGKASNAGGVATSGLEMTQNSMRLPWS
                     AEEVDQRLRGIMKSIHDTSLHTAKEYGHPGNYVVGGNIAGFLKVANAMMDQGLV"
     gene            complement(2326..3354)
                     /locus_tag="PK754_00760"
     CDS             complement(2326..3354)
                     /locus_tag="PK754_00760"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNB08065.1"
                     /translation="MKSKILALAMVLLYASSTFAGEEKRGTAGFMFLKVPMGAREVSM
                     GQSGITTSMGANAIYWNPANVSMATGPSFQVSYLNHFAGISTNYAALSFPMSDIGTFG
                     LSFNYLSYGDITKTDELHPDGNIGNYSAYDLAFGFTYSKQITDRVNGGMTVKFLNSHI
                     DQVDASAYAFDFGFTYNTDFKGLKMSFVVSNLGPQSTYSGDGLLRETTDPLTGATTYF
                     AYESEPFNLPAAVNFGLSMDLMRNEQNSVTGMLEQNVNSFQVSRTNFGLEYGFQNMFF
                     ARAGYSSALKKDRDYNTGNDAGMSGLTMGAGVDYKFNDSFGATVDYGYLNMGLLGGTH
                     RFSVGLKF"
     gene            complement(3463..6987)
                     /locus_tag="PK754_00765"
     CDS             complement(3463..6987)
                     /locus_tag="PK754_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08066.1"
                     /translation="MNKRKLIWLLTFVSVVTAVVVSSLYDVSNAKVDTATKLKLRLKK
                     PDIIDLAYVGYNNWSYVMRNNGSFMYDSPDQDGNGNNAGGEFPRGSGTTIVFAAGAWF
                     GTMKNNREVVSECQFASEFQPGRIENSGVPFEELTAEDFLGTEREVYLIDRSGSGDDY
                     NNWPSDAPVDQFGLPGLIADAQTWVVFNDLDTNRSQEGPADSPFPGLGIQITLESFAF
                     NAGSLSNVVFLKFTFDNKTNSDYPNSYLGLWMDADVDNSSNDVIGSDSSKGLGYVYNV
                     DNTDQPYATGFDFFQGPVVSASEVSSFLAFKNADNKTVLKYNPVQGRYVPTTLGGDSV
                     WLGATTLNTYPNSGDPNNNLERYRLLQGIDRDGSVNPNSFNGWYYPGNPLTGIVNVHT
                     VGNDQRILHGVGPFVVKAGAKQEVWAGVIAGIGTDRLSGVADMFKTDVLAQTTFNAGL
                     VAPAPPLTPKINVTGLDGRVNITWSDNAELTYDNAGDVLGIKEDSGYTADYLANDFQG
                     YRVYRSLTGLPGSYERLAQYDKADHITVIRNFTLNTRGSLDIQDINVGDDTGLRYFYV
                     DENVTNLTTYYYSVTAYDAQPYIADEDVLFHDADLGVDIPNPTGLPISLETSPTSNVV
                     SVVPMAATAGSSYDASVTASATHTGPSDGSVDLEVVDRAAVTGHNYRVEFFTIPDSVD
                     GYDLIHPDQGDIAYRVYDITDGVYKKFSTRADNPDTYLDTNANGDKDATDLVWDDSYY
                     ATAQASAADATTEQFHVVDGILVKIFGPALDVKDFLTVANAAGPLNPPVYAAYAFNGS
                     GFPSAQPGFDPVNGVLDRPTDDQQSTNNTKWGFNVGGAGTNFHYNDGTTTSFVARVFR
                     GTNFSRFVPYDFELRFTAAGGQGNFFFSSGNQGAVPFEIWNVGIGTPDNSADDYRMYP
                     RVFDEGGAVDVWDITEFDHPISGGDNDPYTDWIYWMRPTNTNPGQAGYDAVVTAGDGT
                     TGINAEVMARVILVNWNGGSVSDPSYPANLVANRPETGTVFRIVSTKPNQTTDQFAFS
                     TTANTALTSKSAKKNSLDKILVVPNPYYGRSTYQKTLFDKQVMLTNLPPVCTIKIFNV
                     AGDLIATLNHNGASNNKRVNTSPLNLATEASSKTTGAEVWDLRNTNGEFVASGMYIAV
                     VEASGYGKKMVKFAVIQEKTTINGPDIR"
     gene            complement(7083..10151)
                     /locus_tag="PK754_00770"
     CDS             complement(7083..10151)
                     /locus_tag="PK754_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB08067.1"
                     /translation="MSGSTRLKRLLLVATMVFLLPMYLFAGNTGKITGVVKDKESGDA
                     LPGVNILIEGTSMGATTNAKGEYTILNVPAGVYTVATSMIGYTKLTKQNVRVIPDFTT
                     RLDFEISATSVTGEEQVVVAERPLIQKDQTMTMSVTTAEEIKNLPVRGFQAAANLGVG
                     IVVSNARNLDGGTANVNIRGGRSSETGVYMDGFQQNNLLTGTSNANVPSGAVEEVQVI
                     TGGFDAEYGRNQSGIIQVTTKSGGQRYSGNIEAVGDPAGLGIAESYGYKVLSGGIGGQ
                     VIPGNNTIRFFVSAEAREIDDAEPSVWGYPEYSISKALSPANYYDHDGDPSTPTVPKD
                     TVTWKTNKDGSVKYKKGARPDGVAGAGINSDKGVNLQAKVTFDAIPNKLRIDLTGNFG
                     QTIRRNTTVARTLAVNDNLRREISNLNVGTIATYTLDQKSFVDFGINYFSNTRKLMND
                     LLGFDNVYDYRTRQLGSTGAPTYFNRNVLQDVNRGSLNYRQDVDQYIAFKGNYTNQID
                     KHNQVKIGADFFRHTIRYLNIGDIGNPITGSNDNIGYKLVEKNGKAVIKEVNSDDLEN
                     KQQGPPHPISLSLFGQDKLEYEGLVIRGGLRLDVFNPGVKRVRNIGDPTGSKAEDQQL
                     VFNDLNLNGEFDRASEIATDGYTDRNDNGNYDEGTDRLHAGSIGAEDYTNAKNDVKIS
                     PRFSVSFPISEKTQFRMSYGKFFQQPNLQDLYVSPYYLEGQSTLSFAAATIGNPNLVA
                     EKSTQYEVGIRRALTEKVALDINAYYKDITGLVNSKAVPSEPTGLIIYDNEDQGVVQG
                     VNIGLEVRRVGKFSGRVNYTLQSATGSGSGTNSGFRAAWLGFSDTKFNAPLNFDQRHN
                     INASLDVRNGKGEGPSVGGVKALENAGLNFLVSAGSGFPYTPTTSGALQVFGVPANKV
                     TARRNSQNQPWTFRIDMKADKTFYVSDNMNINVYVQVLNLLDRKNVLVVYPSTGRADD
                     NGFLDTEGFNSTYATQAIRDQVRPQYDVNFHDGFSWDTPRQARLGILFNF"
     gene            11036..14851
                     /gene="smc"
                     /locus_tag="PK754_00775"
     CDS             11036..14851
                     /gene="smc"
                     /locus_tag="PK754_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNB08068.1"
                     /translation="MYLSRLEVFGFKTFAQKVDLRFDEGITNIVGPNGCGKSNVVDAL
                     RWSLGEQKASILRSEKMENVIFNGTKQRKPLNFAEVSLTIQNTKGILPTEYTEVTITR
                     RIYRSGESEYFLNRLPCRLKDINDLFMDTGMGADAYSVIELKMVEQILSDKTEDRRKM
                     FEEAAGITKYKIRRRQTFNKLQTTKLDLNRANDIIVEIEKKVNSLKRQTQKAKRHMKL
                     SARLKLAEVQLAHHEYQKFAAQLAPMAEKLENLEFVAEQMLGEISKKEAEVEAMNTGL
                     IEREQSLREIQLQLEEQDKKIKTIEDEILVSRERKKNLEAMIRRYAEEKTALEAKREQ
                     LQARITELSATLADLESQYNGMSGTVEAKKTEMLEFDQQIQAKRTELDTVRKQSVQLI
                     DEVAKKRNAFQLTKNNIQNIERKIADLHREGEFHNTSSEDAISALDEKRYEKKKLGEQ
                     VEALRNKITADQSALDTLRHTLDDLRSKKLHDEAEIKALHAKMAIIQKAIENHEGFPE
                     SVQYILSSKPSGIQATVADMIATDDTYKKAIETALGDSFAYLVAGGSDHVHDALTQLN
                     TNKKGSATFLNRQKIGQFKPAFEAEALASVRGEIIGMATELVRCDDPAMARLLLSDVV
                     IVENFEKATRLADQFPAVRFVTLQGEIVKGNYLIKGGGTVKSSDSLIGQREALNKLQD
                     QLSQYEASVRRYQDELQTTDTTLRELTQTIANNQNLLKNTEQKFVALEKEIAQSEYEQ
                     KRSRESLTRNEDALLQAQREMEAFQLTIEDMGPEMDELEGRRGSLELETRRMEQQLAE
                     LEKQRRERSEGFNEMISSFLRMEGEIKTNRNNIESSRTQIADIEATILRREEETVAAE
                     NEIARMGDVFTEREGELITLSRERDVIERNRDAEGTEVARLRETTGKIEAELKKMRRQ
                     REEFLNSRHTMEQETTNLRFELRALEERIKRDHDFDLTKDSIETLILSTEPGSKEEES
                     EEDAPESAPDTAPQEVSGVENQEMNIDEVLESDLVSDASQAPETASFDPEATKLEIDE
                     LRRKLKSIGPVNMEAFAEFQTEKERLDILLKQRQDLLDAEAQLLETINAINEQAQKQF
                     MEVFEKIRANFIKIFTGLFVDSEADLKLEANEDILEANIDILARPTGKKIQHIQLLSG
                     GEKTLTAISLLFAIYLVKPSPFCILDEVDAPLDDTNIDKFTKILKDFSKDTQFIVVTH
                     NKRTMEAARNIFGITMQEAGVSKVVSVKFSNRGDVSKTDDIEQFINENQVEDLTKEVK
                     DTPPSPSVA"
     gene            15100..15741
                     /gene="nth"
                     /locus_tag="PK754_00780"
     CDS             15100..15741
                     /gene="nth"
                     /locus_tag="PK754_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNB08069.1"
                     /translation="MESIDFKKQRAQRIVRSLARSYPDARCRLTYHDAFELLIKTILS
                     AQCTDERVNFVGETLFRKYRSPEDFIQVPQAELENDIRSIGFFRSKARHIQTCCRTLI
                     QNFNGRVPSTMDELITLSGVGRKTANVILGNIYNIPSLAVDTHVIRISRLLKLTRHTE
                     AESIEADLMKILSPKDWVMFSHRVQMHGRTVCIARRPQCDHCSLAKDCPSANK"
     gene            15746..19171
                     /locus_tag="PK754_00785"
     CDS             15746..19171
                     /locus_tag="PK754_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB08070.1"
                     /translation="MNLTRRLLIIAFFFSAISAFGQRLFVRHYSTAEGLPQNQVFAIH
                     QDQHGFLWFGTGGGLSRYDGRQFRNYTKEHGLASNVIRAIHRDKDQNLWLATDEGISC
                     YVAQRDTFISYHSDHIGQGTVRAIAGDDRRLWFATAGGLTRYDFNDHSFTNFTTANGL
                     PANLVQAVTVTAYGKVFIGTNQGLAVMSDPLSDRIEQVPIPGNNKLLLRVEALYCDAR
                     NRIFAGTQGGVIRIDSLKPEIISTADGLTNPVIRGITSDQSGHIWFATEDGLHVMTRS
                     ERPYLFNSYTTRNGFFANQYYAVFQDSENNLWFGSSRGVSKLISADLISYTEAEGLGN
                     DVVIGITKATDKTWYITTNDGISIFREGRFQNLSDTEGLIAKEIWDAATDSAGVIWLA
                     TYRGLQALLPKEPRTRLPIHTKLPRAGIVSSGYYNLTLPPNDFIHNNRIVDVLVDHRG
                     RIWFASTDKGTGYVEINSTGEISVRTFTTADGLYSNNVWMIYEDRRGQIWAGTIGGGI
                     ARWDEVSQRFTSLNKGRGLSDDVALSIYEDRRGGLWIGTERGAMRLAPEIVSGDSFPG
                     DLRSYIRLVSKRDGLSDNTINAIVEDSEGMIWLGTNNGLNQFNPETGLTLQVYNKKRG
                     LIDNEVSTQNALRFYDHETLIVGTSAGITLLPLRSFRSPLPTRPVVLMQEFMAEDNAN
                     KTARLIPIAMRPDTNVSDAFFSTLRTSVPVVSYLENNITFRFVAPSFKDENDVRYRYR
                     LRGFDRFWSDPTPENHVRYTNLNDGDYVFEVIAGNGNGAWSDVPTRVTFSVMTPLWKS
                     WWFITFSITFFSLSIYTAYKFRANIARQRTAELEQKVLQRTRELVVQKETVERILNEL
                     KETQIHLIHSEKMASLGQMVAGIAHEINNPVAFVKGNISLLERRLHDLERVFTSFSDV
                     FDFYDKFNAFSDDLHKDFRAKLEEIDRIIASTRFEKFLGDLPQVVNEMRDGVDRTQKI
                     VEDLRNFSRLDESQFKEISIIDSIESTLNILKNEYKTRITIHRNFGDVPPVYCNPGHI
                     NQVFMNLLQNAFQAIEGDGNVWIRVVAASNNILIAIRDDGKGIPGEIQNRVFDPFFTT
                     KPVGKGTGLGLAISYKIIEAHKGTIFFESTPQQGTEFKITLPIRRLADLQKPE"
     gene            19272..20561
                     /locus_tag="PK754_00790"
     CDS             19272..20561
                     /locus_tag="PK754_00790"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNB08071.1"
                     /translation="MIKKASLSYDDKVLDLPLITGTENEQAVDIGKLRGATGLVTMDP
                     GYGNTGACHSGITFLDGEKGILRYRGYNIQDLCESSNFQEVSYLLIHGELPSAQQLEE
                     FKRGITRHTMLHESLRKLYDAYPRDAHPMALLSSIVCSLSTFYQDSIDPLNKKHVEIS
                     IFRLLAKIPTIAAYAYKKSIGQPFMYPNNSLSYCANFLNMMFAVPAEPYEVDPEIEKA
                     LDLLFILHADHEQNCSTSTVRMVGSSQANLFAAISAGICALWGPLHGGANQEVLEMLQ
                     EIHNDGGDVKKYVEKAKDKNSTFRLMGFGHRVYKNFDPRATIIKKTCDRVLSKLGVND
                     PLLDIAHQLEEAALKDPYFIEKKLYPNVDFYSGIIYKAMGFPVEMFTVLFAIGRMPGW
                     IAQWKEMMESKETRIGRPRQIYTGSAERPYTPIHKRG"
     gene            20711..21145
                     /gene="dtd"
                     /locus_tag="PK754_00795"
     CDS             20711..21145
                     /gene="dtd"
                     /locus_tag="PK754_00795"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018672208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNB08072.1"
                     /translation="MRIVAQRVNKAHVSVDGRITGAIEKGLVLLIGIKNGDTSADVKF
                     FAEKCLHLRIFEDAEGKMNRSIIDAKGSILAVSQFTLYADTTKGRRPNFLEAARPELA
                     EPLYNEFVTHLRSHNIHVETGIFGAMMEVEIHNSGPVTIILE"
     gene            21155..23719
                     /locus_tag="PK754_00800"
     CDS             21155..23719
                     /locus_tag="PK754_00800"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HNB08073.1"
                     /translation="MNFFPRISLLYFHLSHLIVLSFFTTLAQTVSDSSFIATQKDSSQ
                     TPGNGVDTMIYYGADTTEGTLNNSTVVLKNNAWVRFKGIEIKAAKITIDQPRKIMIAE
                     AVPDSVDSLGQVARYRGIPEFTESGQMFHGNVMEYNFETKRGKVVMGETKMQDGFYYG
                     STIRKIGDSTLYIRGGRFTTCDEKEPHFFFRTKEMKLTVRDKVVAKPVILYIHEVPIF
                     AIPFGVFPSKGGRASGITPPVYFETPGGERQIRNFGYYWAPNDYFDALLQADYLDKSG
                     YQLHGGGNYAKRYHYSGRMDFSYSQLHSLSTTSYNDNRTTAGALSLTHAHTLSERSNL
                     NADIRYQSSKNYFQNTSINQQQILNRQQNSNISYNNTFDWGGISASASHSRNLDNGQS
                     SLTFPNATISKSSSSVIPKSEERRNEPDRWYHTIRYSYNANLLGRREIANDTSDVQNG
                     YGLRHNAGFNAPFKVFSYFTFNPNINVVETWYDRRHENFGYRANGQDTSVVKRGFYAT
                     HTYSMGASLSTKVYGTSNPNLLGLKTFRHVMTPSVSINYTPDFSESKWGYYESVQDTS
                     GKTVRKDRYGKNLTFGGTPSGRQLSMGFGLAHTFQAKVKTSKKDSTQRDPDVRKIDIM
                     NINNGLSYNFEAEEFKLSNLTTSIAVANDLAKNVSLSMNLTHDFYKFDTTLNRRINKF
                     RNFPRLINAGITGGFSIEGGSGASSTTSGSTPSTTASTTPQGTVTPNYGQSYLPSQRE
                     LPSGVPWNARFNFNYDIGRLDPRNVTKTFSGNISAGTRITQNWQVNYSAQLNLLKEKI
                     VSQSFSLVRDLHCWTMRFDWTPTGPAAGYFFVIQVKSTQLQDIKLQRTDYGNRIFQ"
     gene            23821..24207
                     /locus_tag="PK754_00805"
     CDS             23821..24207
                     /locus_tag="PK754_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNB08074.1"
                     /translation="MNVLNSTFLLRLAVSITLFMHSVPGMFNGGVNNFGTFYLNEVGF
                     APLGVPLAWAIKLSHVAAIFCLMFEKYVKLASLVTIFVLLAGIVMVHYPDGWYVVGGG
                     RNGMEFNVLLIFVLLAIMFPNGLKKN"
     gene            complement(24283..27021)
                     /gene="uvrA"
                     /locus_tag="PK754_00810"
     CDS             complement(24283..27021)
                     /gene="uvrA"
                     /locus_tag="PK754_00810"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNB08075.1"
                     /translation="MIEIRGARVHNLKNISLNIPRNKFVVITGVSGSGKSSLAFDTLY
                     AEGQRRYVESLSAYARQFLERMDKPEVDHIKGISPALAIEQRTLAKNPRSTVGTVTEI
                     YDFLRLLFGRAGKTYCRNCGALVRKDTVGYVVQRILALPERTKLHILFELAANEQTIL
                     SLHEKGFTRLIVDDVIAELNDEAVVKKILKQKIVRVLVDRIAVNKEEKARVADSVETA
                     FLNGEGKMIVQIMEGETLRFSNKFECNACGTAYIEPDPRLFSFNNPYGACPSCQGFGN
                     KIEVDMDLVIPDRTKTLKEGAIKPWTTDGYADFQERLEKAAKKNGIPLNIPVMDLTKE
                     QYEKVMQGGDGFDGIREYFKWLESKTYKMHVRVLLSKYRGYVTCSDCGGSRLRPDANC
                     VKVADKTIHEINDMTVGEAVHFFVNIQLSEMDREIARRPLEEILKRYNYLKNVGLEYL
                     TCNRLAGTLSGGEAQRISLATSLGSALVGSLYILDEPSIGLHPRDNDKLIHILKNLQA
                     IGNTVVVVEHDRDMMASCHEIIDMGPRAGRYGGEVVFQGDHAAICQSASSLTGLYLSG
                     RKEISLPSERRQGRGQSIVLKGAAENNLKRIDVQFPLGMFVCITGVSGSGKSTLIHDI
                     LYAAAMRKKGQWSNKIGLHKEILGVEHVDAIEMVDQQPIGRTPRSNPATYVKVFDLIR
                     DVFAATPTAKMRGYQSGTFSFNVPGGRCEVCEGAGQILVEMQFLADVYLECEECKGKR
                     YKKEVLEVHFHDKNIHDVLEMPITEALAFFKAHPKIVKKLQVLDDVGLGYLKLGQAGT
                     TLSGGEAQRVKLAAHLADKSGNHVLYIMDEPTTGLHFEDINKLLTSFNKLIEAGHSII
                     VIEHNLDVIKCADYIIDLGPEGGDKGGMIVATGTPEEVIQVGNSYTGKHLKPYLKK"
     gene            complement(27035..28027)
                     /gene="waaF"
                     /locus_tag="PK754_00815"
     CDS             complement(27035..28027)
                     /gene="waaF"
                     /locus_tag="PK754_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HNB08076.1"
                     /translation="MEKILLSISAGIGDALMAQPLLCEIQNNFPQSHIEVLCSAATQH
                     VFRSHPGIQKIWTMDVSSIKKYFGLVGKIRKEKFDLYIGAIPSNTYSQLLLPVLAGIP
                     VRIKHNSPHKFPRNLDALYHALTPIGEGVHRLECNLELLHFLKVNHWSKQSPKFHISD
                     VTLAKMETRIAHDRKKLIIGFHPGCNPAAAFKRWPSERYAALADYLYTTYDATVFIVG
                     GKDEADDVRKIMEQAQHKPLNFAGQCTLEETAALIAQAHFFVSNDSGIMHLSTAVGTP
                     VFAIFGPKDERHIGPYGEKHTVIRNGRDVNQVTLEQVVQTLIASKFGLKPFIKS"
     gene            complement(28049..29902)
                     /gene="asnB"
                     /locus_tag="PK754_00820"
     CDS             complement(28049..29902)
                     /gene="asnB"
                     /locus_tag="PK754_00820"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNB08077.1"
                     /translation="MCGIAGVMNFTGEPVNESLLKAMGDALAHRGPDGDGFFIRQHVG
                     LAHRRLSIIDLKTGQQPMYDDDGKSCIVYNGETYNFGEVREELIKLGWNFRTQSDTEV
                     ILKAYLQWGASCLDRFRGMFALAIWDERTKSLFLARDRVGIKPVYYYADQNKFLFGSE
                     IKAILQDKSIDRTIDYSAMREYLQYGYVPSPASIYNKIKKLPPAHWLHITFDGGRYNM
                     KSKCYWDIQFGQDTDGAHNDTTWIDKTESVVSDSVRIHMISDVPVGAFLSGGIDSTIV
                     SHFMAKHVGTINTFTVSMNESRFDESAYARQVSAIIQSRHVEKSVQAADLNTILSNLV
                     YAYDEPFADSSAVPTYQIAKIIRSHVTVALSGDGGDEVFGGYKRYEQLLEWSQSNPWL
                     NKVWLSKIATFWPRNIPGKNYLRYHSQSDMIRYYGMINIFHSDQIADMMKFSVEHRER
                     LFDSYMNSSDALLNKMQNIDIHTYLPENGLTKVDRASMAHSIEVRVPLLDHKVIELAA
                     QMPVSMRMRKSEKKYVLKKILEPRFPKELIYRTKRGFSLPMRDWFKSNPDLTRMIHHE
                     IKECELIKEHMNLHFIEAMITTHLNSSKNHASRIWSLWFLARWYDTYYKKM"
     gene            complement(29923..31149)
                     /locus_tag="PK754_00825"
     CDS             complement(29923..31149)
                     /locus_tag="PK754_00825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB08078.1"
                     /translation="MKILHIISNGNIGGREKVLYLLLNEESKDKQIQTSAFFKYPVGP
                     YYTKIKSLGISVYYNITRLRDIISLMEKQDIIVFHFVDWRFFLAGILSGKSILYRLSG
                     IYLLNKKSLGDVFFILAKKIKKTIWRRHSEDDRRGDQLIKLPAKENTSQSKIPNRRSL
                     YRLIKRKYFELFLRKWCTYLIANSRYTAKSAVEKYGIQPQKIRIVMNGIEFNNRKGDE
                     TDFRASYNIDKNVFLIGTVCRFDTRKRLDRLLMGFSMISNLKDFHLVVVGGGDTVIEQ
                     ALKQFVAHNNLLDKVSFTGILENSSDAMSAMNLFVLSSDNETFGVSLVEAMYKKKPVV
                     VFSDSGGPAEIISGQNCGYIINTPSEMPGIVEQLRADPILTQRLGENGHRLVVEKYNV
                     KRFYKNSLRVYSELFE"
     gene            complement(31146..32150)
                     /locus_tag="PK754_00830"
     CDS             complement(31146..32150)
                     /locus_tag="PK754_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08079.1"
                     /translation="MRRTVFFIVSIDTECDKSIDWSITYPFSFRNIHEGVAEKLQPLF
                     DIYKVKPTYLLSPEVIYNQESVNYFKSIQDRCELGTHLHGELIGYDKLENPRWTNDFQ
                     CDYDEGIEREKLESLTELFKNKFGYMPKSFRAGRFGIGPNTWRILAELGYAVDSSLLP
                     FQLIKTKNATYNFYYTPVKPFYPDLQNWMNFEKQEIRKILHVPMTVHNWFFQRIPPCI
                     GKPLSEQNRIRSLFRILLTKEKEKTYSLRPRTDNAESLINLIKHHIKIHTTKEPIVLN
                     MMFHSNELHPGASPYAQSPLEVGQTINTIKRILDYINRYYSIHSIGLCDSTRYYERDT
                     "
     gene            complement(32191..33444)
                     /locus_tag="PK754_00835"
     CDS             complement(32191..33444)
                     /locus_tag="PK754_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08080.1"
                     /translation="MMGNDAEINEYSLQNLRKYFLVSIQNYLEILMLMLLVPIVWLIS
                     NPYDFIASNDPWEYFNISHGLIIGNGIVLNDNYDVILPPGYPVIIGLLYFLIPVEWPI
                     LLGIGIVSFILSIILMKKILQLMGFNFHLRIIGLFFFAFNSQLLIQAGRGYSDLLMIL
                     LILSLIWVSIIKKNTKNLSWGIVLGMLWGLLSLTKPEGVLLGLVIISLSVFNSRMIKG
                     IAAICFIVLYLPYGVFLKNQTGAWQISGKTYVNLVLGELNSPYQNPTNYDTRYAVISR
                     VWENAEEAGGTSVYLINLGYKILERVPYNIKRFSNYFVSTYSWIGGCFLLACVIFWNS
                     IYRRFIVLSIGFTCFYLFFFIIPRVLTLYHVFFSFAMLAGFQIILTHINRVRNRWLTL
                     SFYGAVVFFISFQFRIVLKILMQKI"
     gene            complement(33441..34145)
                     /locus_tag="PK754_00840"
     CDS             complement(33441..34145)
                     /locus_tag="PK754_00840"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB08081.1"
                     /translation="MNDKHHYDGISVDQYDNRKNRNVFEKYLYDHWHPIIVEHIRLLC
                     DKRDVLDLGCGSGAYIGFALESTCKVSGLEVSQNMLNAAKKRYPKADFILGDAQETRV
                     ESESFEIIYSIGLLEYVNVENTLKECYRILKSNGRLMILTPNKYGGRKFVTRYLKGKK
                     KSSPKYYSRREVLNALDKQGFYIDKVIMDDGLIYLPNITPDIISRLIFRTNETFFKLF
                     PYNPFSQNMIFIATKR"
     gene            complement(34138..35694)
                     /locus_tag="PK754_00845"
     CDS             complement(34138..35694)
                     /locus_tag="PK754_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNB08082.1"
                     /translation="MDNARQKDITKLAKKFAISGIGSIFGLFWTPLTSVITTRFLGAE
                     LYGIFSLVQSWGSLLANLSSIGLNGMNLRFIPTYKATGSMALIKGSVFTTIRVSVVIS
                     AAATCFILFFPDLFCELFIHRPETISEEYFSNYVTKAFQFYAVSILLTASYQAFLSSL
                     VGYQFVQYKVLANDVLGPAAKIISLTSLLLLDFNLYAALGSNIIQDIVVLLVSAFYLF
                     RIAPELRDKSIPVQIEKKKMMKFSSALFANSLLSKYTYQLDILFLGYYATLPEIGIYT
                     LALRLQPLIYLPHYSISSVFGPMVAELYTAGKIKELNQFYKTTTKWSVTLSLPICSLI
                     ILFSKEILGVFGKEFAAGFELVAILSIGNLIHDFLGLSGNIILMTGRIRINVINSIVM
                     AIINLLLMSILIRNYGMYGAAIGNFLSITILSILTTVETFYILKIHPFDFLLLKPFFA
                     MLISSSIVIAISIWYRLPYYEITFLIYILVFILLYVGFLFLFGLSQDDRMILDKILKK
                     LPFKKQNSNE"
BASE COUNT        10550 a         8205 c         7191 g         9952 t
ORIGIN      
        1 atctatatgg atggttttgg tgaaaaagct aaaaaactgc actccattgg catcaggaca
       61 cacaccatta aaacaaatta ttggactaaa aataccaatg cggactccac ctatcgtgaa
      121 ttgttgggtt ttaaatgata cgagctattt tttttgacct tggaaaagta cttgtagatt
      181 ttgattggaa tactgcggct gaacatattt ctcgcaagtc tgaactcaaa atcactgaaa
      241 tcatttatcg tagcacagcc agcgacgtag ccatgtcatt tgaatgcggg caattagatt
      301 caaaagagtt cctcgaaaaa ctaaaattga gtttgagatt tcgaggaaca accgatgaac
      361 ttcatttttt gtggactgaa atttttaccc ccattcataa taacatcgaa ctgctttatc
      421 ggttgaaacc aacttatact ttaggtttga tttccaatac aaatgcactt catttcgatt
      481 atatccgcaa cacatatgat tttaccaaag aatttgacag actaatacta tcatacgaag
      541 ttggtgcgat gaaacctgct cgacaaatct ttgaaaaagc cattgccggt atgaaccttc
      601 gctctgaaga aattttattt atagatgata ttgcgaaaaa tctagatggc gcaaaggact
      661 taggttggca gactatacat tacacgggtc acgacgaatt gatgaaaaaa cttcaaactt
      721 ttatggttat catttaaaat agaaaagagg ggttcgattg aacccctctt ttcttgttag
      781 taatttcaaa acgcttttat accaaacctt gatccatcat agcattagcg acttttaaga
      841 atccggcaat atttcctccg acaacatagt tgcccgggtg accgtattcc tttgccgtat
      901 gcaaagacgt atcgtggatg ctcttcatga taccacgcag gcgttgatcc acctcttcag
      961 cggaccacgg caaccgcata ctgttttgtg tcatttccaa accggatgtt gctacaccgc
     1021 cggcattgga agctttgccg ggtgaaaaca gaataccggc ttgtttgaac acttcgtagg
     1081 catccagcgt gctcggcata ttagcgcctt cgcaaacgca gatacatccg ttttttacaa
     1141 gctctttggc atcttcaacg ccgagctcat tttgtgttgc gcaaggtacg gcgacatcca
     1201 ctttgacgct ccacggacgt ttaccggcat gatactctac tttgaattca tcagcataat
     1261 ctttaacgcg gtctttgcca cttgcacgca ttttgagcat gtagtttatt ttttcacctt
     1321 gaatgccgtc tttgtcatat acgaaaccat cagggccgga aatagtaact acttttccac
     1381 ccaattcgga tattttaagt gcagcgcccc aggccacatt gccaaagccc gataccgcaa
     1441 aggtcaaccc tttgatgtct ttacctttgg tgcgtaacat ttcttgtgca aaataggtaa
     1501 caccaaatcc ggtagcttcc ggacgaatca aagacccgcc ccagttgata cctttacccg
     1561 tgagaacacc ggtaaattca tttttaagtc tcttatattg accaaaaaga aatcctattt
     1621 cgcggccacc gacgccgata tcccctgccg gtacatccag gtcagcgccg atatgtctgt
     1681 acagctccgt cataaaactt tggcaaaagc gcatcacttc gttatcgctt tttcctttgg
     1741 gatcaaaatc tgaaccgcct tttccaccac ccatgggcaa cgtggttaag ctatttttga
     1801 aaacctgttc aaaagccaaa aactttaaaa ttccaagatt tacggacggg tgaaaacgta
     1861 aaccgccttt atatggtccg attgcactat tcatttgcac gcggaaaccg cgattgatat
     1921 gtatttcacc ctgatcatct tgccagggaa cacgaaacat tactacacgt tccggttcaa
     1981 ccatacgctc taatattttt gctgtacgat attccggatg acgatccaat accggttcta
     2041 atgattcggc tacttcttga actgcttgat gaaattccgg ctcagccgga tttttggctt
     2101 taacttgcgc caaaaccgat ttgacgtagt ctgacatact atgcctctac ctgaattaat
     2161 taaaacaaat tttattcatt tccgcgcaaa tctcgttatt tatactctga aagtcaatat
     2221 tttagagaac aaaagactat atacacaaaa ccaaatacaa tacaaacgta taaaaaaaaa
     2281 gcctcttccc ataacgagaa gaggctttat ctaatgtttt aaatattaga atttcaatcc
     2341 aacggagaag cggtgagtac caccaagtag ccccatgttc aaataaccgt aatctacggt
     2401 tgcaccaaag ctatcgttga atttgtaatc aacgcctgca cccattgtca agccgctcat
     2461 accagcatcg ttaccggtat tgtaatcacg gtcttttttc aatgcggaag aataaccggc
     2521 gcgcgcaaag aacatgtttt gaaatccgta ttccaaacca aaattagtac gacttacctg
     2581 aaaactattc acattttgtt cgagcatgcc ggtcactgaa ttttgctcgt tacgcatcaa
     2641 atccatactc aaaccaaaat taacggccgc aggaaggtta aaaggttcgg attcataagc
     2701 aaaataggta gtggcgccag ttagaggatc ggtcgtttca cgtagtaatc cgtcaccgct
     2761 ataggtggat tgtggaccaa gattggatac cacaaaactc attttgaggc ctttgaaatc
     2821 tgtattataa gtaaatccaa aatcaaaagc atacgctgaa gcatccactt gatcaatgtg
     2881 tgaattgagg aatttaactg tcataccgcc attaacacga tctgttattt gcttggagta
     2941 tgtaaatcca aaagccaaat cataagccga ataattaccg atattgccat ccgggtgcaa
     3001 ttcatctgtt tttgttatgt cgccataact taaataatta aaactcaaac cgaatgtacc
     3061 aatatcagac atgggaaatg atagcgcggc ataattagtc gaaatgccgg caaaatggtt
     3121 aaggtacgag acttgaaatg aaggcccggt tgccatagaa acattagcag gattccaata
     3181 aatggcgtta gcacccatcg acgttgtaat accgctctgt cccatagaaa cctcgcgcgc
     3241 gcccatcggc actttcagaa acataaaacc cgcggtcccg cgcttttcct caccggcaaa
     3301 ggtcgaagat gcataaagca ataccatcgc caatgctaat atttttgatt tcataatttc
     3361 tccttgactt gttttcctct gccggtaagt tatttaccgg cagaagattt taaactacat
     3421 taattcatcc atacgttcga tgctggacga caagttctat tcctagcgaa tatcaggacc
     3481 attgatagtt gttttttcct gaataacagc aaacttaacc attttctttc cataacctga
     3541 ggcttccaca acagcgatat acataccgct tgccacaaac tcgccattcg tattacgtaa
     3601 atcccaaact tcagcgccgg ttgttttaga gcttgcctct gtcgcgagat tcaacggcga
     3661 agtattaacg cgtttgttat tacttgcccc attgtggtta agagtagcaa tgaggtcgcc
     3721 cgctacatta aatattttga ttgtacaaac gggaggtaaa ttagtaagca tcacttgttt
     3781 atcaaaaagc gttttttgat aagtggagcg accatagtac gggttaggca ctacaagaat
     3841 tttatccaaa ctgtttttct tagccgactt tgaagtcagg gccgtattag ctgtcgtgct
     3901 aaacgcaaac tgatccgtag tttgattggg tttggtagac acgatacgaa ataccgtccc
     3961 cgtttctgga cgatttgcaa ctagattggc aggatatgac ggatctgaca cacttccacc
     4021 attccagttt acaagtatca cacgcgccat gacttctgcg ttaatgccag ttgtaccatc
     4081 gcctgccgtt acaaccgcat cgtagccggc ttgaccagga tttgtgtttg tgggacgcat
     4141 ccaataaatc cagtccgtat aaggatcgtt atcaccaccc gaaattgggt gatcaaactc
     4201 cgtaatatcc catacatcga ccgcaccgcc ttcatcaaat acacgcggat acatacgata
     4261 gtcatctgct gaattatcag gagtgccgat acctacattc caaatttcga aaggtacagc
     4321 gccctgattt cccgatgaga agaagaaatt accttgaccc cctgctgctg tgaatcgtaa
     4381 ttcaaaatca taaggaacaa aacgtgagaa attggttcct cggaatacgc gcgcaacgaa
     4441 tgaagtggtc gtaccatcat tgtaatgaaa attggtaccg gcaccaccca cgttgaaacc
     4501 ccacttggta ttattggtag attgttgatc gtctgtcgga cgatcaagga caccattgac
     4561 aggatcaaag ccgggttgcg cagatgggaa tccgctacca ttgaatgcat aagctgcata
     4621 cactggggga ttcagaggac ctgcggcatt ggcaacggtc aagaagtctt tgacatccaa
     4681 agccggacca aaaattttga ctaagatacc atctacaacg tggaattgtt cggtcgtagc
     4741 gtctgcggct gaagcttgtg cggtggcata atacgaatca tcccacacta aatccgtagc
     4801 atccttatcc ccattagcat tggtatcaag gtaggtatca ggattatcgg cgcgtgttga
     4861 gaatttttta taaacgccat ctgtaatatc atacacccga taagcgatat cgccttgatc
     4921 gggatggatc aggtcataac catctacgct atccggaata gtaaagaact caacgcgata
     4981 attatggccc gtaactgcgg cacgatcaac aacttctaag tcaaccgatc catcgcttgg
     5041 accggtatgc gtcgcgctgg ccgttaccga agcgtcatac gaagagcccg ccgtagccgc
     5101 catcgggact acactgacca catttgatgt cggggaagtt tccaagctga tcggtaagcc
     5161 ggtcggatta ggaatatcca cgcctaaatc ggcatcatga aataacacat cctcatctgc
     5221 gatatatggc tgtgcatcat aagctgttac agaataataa tacgttgtga gattggtgac
     5281 attttcatct acataaaaat aacgcaatcc cgtatcatca cctacattga tatcttggat
     5341 atcaagcgaa ccgcgtgtgt tcagagtaaa gttacggatc acggtaatat gatcggcctt
     5401 gtcatattgg gctaaacgtt catacgaacc gggaagtccg gttagactgc gataaacacg
     5461 ataaccttga aaatcattgg caagatagtc cgccgtataa ccgctgtctt ctttgattcc
     5521 gagcacatcc cctgcattat catatgtcag ttcggcattg tctgaccacg taatattgac
     5581 gcgaccatcc aagcccgtca cattaatttt aggggtaagg ggcggggccg gagctacaag
     5641 tcctgcatta aaagtcgttt gtgccaaaac atcagtcttg aacatgtccg caacgccact
     5701 taagcgatcc gtgccgatcc cggcgataac gccagcccag acttcttgtt tagcgccagc
     5761 tttcaccaca aacgggccta caccgtgaag aatacgttgg tcgttgccta cagtgtgtac
     5821 attcacaata ccggtaagcg gattcccggg ataataccaa ccgttaaatg aattcggatt
     5881 tacgctacca tctctatcaa tcccctgaag taatctataa cgttctaaat tgttattggg
     5941 atcgccgcta ttaggataag tgttgagggt cgtagcgcca agccataccg aatccccacc
     6001 taatgtagta ggtacataac gcccttgaac aggattgtat ttcaatactg ttttgttatc
     6061 tgcgttttta aaagctaaaa aagacgatac ttccgacgca cttaccacag ggccctgaaa
     6121 gaagtcgaaa cctgtagcat acggttgatc tgtattatca acattataca cgtatcccaa
     6181 tcccttagag gagtccgaac caataacgtc gttagaggag ttgtccacat cggcatccat
     6241 ccataaaccg agataactat tcgggtaatc agaattggtt ttgttatcaa aggtgaactt
     6301 caaaaaaacc acattagaaa ggctacctgc attgaatgca aaggattcta atgttatctg
     6361 aatacctaaa ccaggaaacg gagagtcagc tggaccttct tggctacgat ttgtatccaa
     6421 gtcattaaat acaacccagg tttgcgcatc tgcaataaga ccgggtaatc cgaattgatc
     6481 taccggagca tccgatggcc aattattata atcatcaccg ctgcctgagc gatcaataag
     6541 ataaacttct cgttcagttc ctaaaaaatc ttccgccgta agttcttcaa acggaacgcc
     6601 tgaattttca atgcgtccgg gctgaaattc agaagcaaac tgacattcag atacaacttc
     6661 tctattattt ttcatcgtgc caaaccaggc tccagccgca aatacgatgg tggtgcctga
     6721 accacggggg aattctccgc ccgcattatt gccatttccg tcttgatcag ggctatcata
     6781 cataaaagaa ccgttgttac gcataacgta actccagtta ttatatccga cataagccaa
     6841 atcaataata tccggttttt tcaaacgtaa tttgagtttt gttgccgtat caactttggc
     6901 gttgctcacg tcgtacagcg atgaaacgac cacggccgtc accactgata caaaagtaag
     6961 aagccatatt agttttctct tattcatact tgtatgctcc tacaataata tttttaatat
     7021 ctaaaaatcg tactcaagtc gggccggatg gcccggcttg agtaagaagc aaatccaacc
     7081 atctagaaat tgaacagaat acctaaacgc gcttgacgag gcgtatccca gctaaatccg
     7141 tcgtggaagt tcacgtcgta ctgtggacga acctgatccc gtatagcttg agttgcatac
     7201 gtggaattaa agccttctgt atctagaaat ccgttatcat cggctcgacc ggtggagggg
     7261 tagacgacga gcacattttt gcgatctaat aaattcaaca cttggacata aacattgata
     7321 ttcatgttat cgctgacata aaaggtttta tcggctttca tatcaatacg gaatgtccac
     7381 ggctggtttt gtgaattacg gcgagccgtg actttattgg cagggacgcc aaatacctgc
     7441 aaagctcccg acgtcgtagg cgtataagga aaaccgctac ctgcggaaac taggaagttc
     7501 aggcctgcat tttcgagggc tttgacgcct ccgacagaag gaccttcccc tttaccatta
     7561 cgaacatcta ggctcgcatt gatattgtgg cgctgatcaa aattgagagg cgcattaaac
     7621 ttcgtatcag agaatcccaa ccaagctgca cggaaacctg aattagttcc ggaaccgcta
     7681 ccggtcgccg actgtaaagt ataatttact cgacctgaga acttaccgac gcgacgcacc
     7741 tcaaggccaa tattcacacc ttgtacaaca ccttgatctt cattatcata aataatcaag
     7801 cctgtgggct ctgaaggaac tgctttagaa ttaaccaatc ccgtgatgtc tttgtaatag
     7861 gcattgatat caagcgcaac tttttcagta agcgcgcgac gaatacccac ttcatactga
     7921 gtgctcttct ctgcaaccaa gtttggattt cctatagtcg ccgctgcaaa agaaagcgtt
     7981 gattgacctt caagataata aggagaaaca tacagatcct gaagattggg ctgttggaaa
     8041 aacttcccgt agctcatacg gaattgggtt ttttcagata tcgggaaact gaccgagaaa
     8101 cgcggactta ttttcacgtc attttttgca tttgtataat cctcagcgcc gatcgaaccg
     8161 gcgtgaaggc ggtcagtacc ttcatcataa tttccattat cattccgatc cgtataacca
     8221 tccgttgcaa tttccgaagc acggtcaaat tcgccattaa gattaagatc gttgaaaaca
     8281 agctgttgat cttccgcttt tgagcccgta ggatcgccaa tattacgtac acgtttaacg
     8341 ccgggattga aaacatccaa acgcagacca ccacgaatga ctaagccttc atactcaagt
     8401 ttatcttgtc caaatagaga gaggctgatc gggtgcggcg gtccctgctg tttattttca
     8461 agatcgtcgc tattaacttc tttgatgaca gctttcccgt tcttttcaac cagtttgtaa
     8521 ccgatattat cgtttgaccc agtgattggg ttgccgatat caccgatatt caaataacgt
     8581 atggtatggc ggaaaaaatc agctccgatt ttgacttgat tatgtttgtc aatctgattg
     8641 gtataattgc ctttaaatgc tatatactga tcaacatctt gacggtagtt tagcgaacca
     8701 cggttcacgt cttgaagaac atttcggtta aaataagttg gcgcacctgt agaaccaagc
     8761 tgtctcgtgc gataatcgta tacattatca aaacccaaca aatcgttcat cagttttctg
     8821 gtattcgaga agtaattaat accgaaatcg acaaaacttt tttgatctaa tgtgtaggta
     8881 gctatagttc ccacattcaa atttgaaatc tctcggcgga gattatcatt gaccgctaac
     8941 gtacgcgcaa ctgtagtgtt acgacgtatt gtctggccga aatttcctgt caaatctata
     9001 cgtaatttat taggaatagc atcaaaagtg actttggcct gaagattaac gcctttatcc
     9061 gagtttatac cagcgccggc gactccatcc ggacgagcgc cttttttata cttcaccgat
     9121 ccatctttat tggttttcca agttacggta tctttaggaa cggttggagt tgatggatca
     9181 ccgtcgtgat catagtaatt tgccggactc aatgctttgg atattgaata ctccggatac
     9241 ccccaaacac tgggctcagc atcatctatt tcacgcgctt cggctgatac gaagaaccga
     9301 atggtgttgt ttccaggaat aacctgccca ccaataccac cacttagaac cttatatcca
     9361 tagctttcag ctataccgag acctgccgga tcgcccacgg cttcaatgtt accggaatag
     9421 cgttgaccgc cggatttagt agttacttga ataatacccg actgattacg tccgtattcc
     9481 gcatcaaaac caccggtaat aacttgcact tcttctacgg cacccgaagg tacattggca
     9541 ttggaagtac ccgtcaaaag attgttttgt tggaaaccgt ccatataaac accggtttcc
     9601 gaagagcgac cgccgcgtat attgacattg gcggtaccac cgtcaaggtt acgtgcgttg
     9661 cttacgacga taccgacgcc aaggttagcc gccgcttgga aaccgcgaac cggcaggttc
     9721 ttgatttctt cagccgtcgt aacggacatc gtcatcgttt gatctttttg aatcaaagga
     9781 cgttcggcaa caactacctg ctcttcgccg gttaccgacg tagcagaaat ttcgaagtcc
     9841 aaacgtgtcg taaagtccgg aatcacacgg acgttttgtt tggtcaactt cgtgtaaccg
     9901 atcatgcttg tggccaccgt ataaacaccg gcgggaacat tgaggatcgt gtactcacct
     9961 ttggcgttgg tcgttgcgcc catggaagta ccctcgatca ggatattcac acccggaagt
    10021 gcgtcacccg attccttgtc ttttaccaca ccggtgatct tgcccgtatt acctgcaaac
    10081 aggtacatcg gaagcaagaa caccattgtt gccaccagca acaatcgttt tagtctcgta
    10141 ctaccagaca taatgtcctc cttctgttgg ttattaagag cgtgtacgat gaatgatttt
    10201 ttttcgataa gtttagtaat catacctttt ttcatttacc tcctttgtaa atgcacatag
    10261 ataagaaatg tgtttttccg acttgacatc tgcttctgag gttaattcta cctacccttt
    10321 cgtttttttg tgaaagaatc agcactacgg ttacaagcgt gccgccctct ccttcacatc
    10381 atactcaagg atactgcttt cagagaacag ttttaagaga acgttattaa aaagatttgt
    10441 ttacaacccg gcaacaagct tttgaaaaga ctagggacac ttattgcaca agataaggtt
    10501 ttacaataaa caatataggc ggccttaaaa ttattgtcaa gctttttttg ggaacccggt
    10561 tttacttggt tttaagacca cgatgggcac tttgagctaa tcaataaaac cttaatcccc
    10621 agataatatt cccgccttag attacaaaac taccgaaaaa atgaaagaaa taagtttccc
    10681 acccccttct gatatttttt taaatttttt cattcatacg tagaatccgg gcaaaacacg
    10741 ctcttttcat gcccatattc gcaataccgg taccgtaata atagaagtac gatgcgttgt
    10801 ggttttatta caaagttttt taaaaagatt ttgcgccagc acgatttaat ctatatatta
    10861 gccgccccaa agcgaaatga tacgtttttc gtgttattta gtatggatat actatattat
    10921 gattagtatg ggtatactat aataatacaa tgttagtttg ctaaaagatg attccctgat
    10981 tgacaggaaa cttatttaag ttttgatttt tttaggttta taaggattat tcggcatgta
    11041 tttgtcacgg cttgaagttt tcggttttaa aacatttgct cagaaagtgg accttcgttt
    11101 tgacgagggt atcaccaata tcgtcggtcc caacggctgc ggtaagtcca acgtagtaga
    11161 tgccctccgc tggtcactcg gtgaacaaaa agccagtatc ctgcgttccg aaaaaatgga
    11221 aaacgttatt tttaacggaa ccaaacaacg taagcccctt aattttgccg aagtcagcct
    11281 gaccatacaa aacactaaag gtattctgcc gacggaatat accgaagtca cgatcacccg
    11341 tcggatatac cgatccggtg aaagcgaata ttttctcaac cgcctccctt gccgcctcaa
    11401 agatatcaat gatcttttta tggataccgg tatgggcgcg gacgcgtact ccgtaatcga
    11461 gctcaagatg gtcgaacaaa tcttgtccga taaaacggaa gaccgccgga aaatgttcga
    11521 agaagcggca ggtatcacca aatacaaaat tcgccgtcgt cagacgttta acaaactgca
    11581 aacgaccaaa ctcgatctca accgcgccaa cgatatcatc gtcgaaatcg aaaaaaaggt
    11641 caattccctc aagcgccaaa cccaaaaagc caagcgccac atgaaactgt ccgcccgtct
    11701 caaattggcg gaagtccaac tggcgcatca tgaataccaa aagtttgccg cgcaactggc
    11761 gccgatggcc gaaaaattag aaaacctcga gtttgtcgcc gaacaaatgt taggtgaaat
    11821 ttccaaaaaa gaagccgaag tcgaggccat gaataccggc ctgatcgaac gtgaacaaag
    11881 tttacgtgag atccaactgc aactcgaaga gcaggataaa aaaatcaaaa cgatcgagga
    11941 tgaaatcctc gtcagccgcg agcgcaaaaa aaatcttgaa gcgatgatac gtcgctatgc
    12001 agaagaaaaa accgcattgg aagccaagcg cgaacaactg caagcccgca tcaccgaact
    12061 ctccgcgacg ctcgccgatt tggaatcgca atacaacggt atgtccggaa ccgtcgaagc
    12121 caaaaaaacg gaaatgctgg agtttgacca gcagatacaa gccaaacgta ccgaactgga
    12181 taccgtacgt aaacaatccg tacaactcat cgacgaagtg gctaaaaaac gcaatgcgtt
    12241 tcaactcact aaaaacaata tccagaatat cgaacgcaaa atcgccgatc tgcatcgcga
    12301 aggcgaattt cataacacct catccgaaga cgcgatcagc gcgctggatg aaaaacgcta
    12361 tgaaaagaaa aaactcggcg aacaagttga agcgttgcgt aataaaataa cggctgacca
    12421 atcggcttta gatacgcttc gccatacact ggacgatttg cgctctaaaa agctgcatga
    12481 tgaggccgaa atcaaagcgc tgcatgccaa gatggctatt attcaaaaag ccatcgaaaa
    12541 tcatgaaggg tttcccgaaa gcgttcagta tatcctatct tccaaaccgt ccggcattca
    12601 agcaacggtc gccgatatga tcgcgacgga tgatacgtac aaaaaagcaa tcgaaactgc
    12661 actcggcgat tccttcgcct accttgtggc cggcggaagt gatcacgtgc acgacgcgct
    12721 gacccagctc aataccaaca aaaaaggcag tgcgacattc ctcaatcgtc aaaaaatcgg
    12781 gcaattcaaa cctgcttttg aagccgaagc acttgcttcc gtacgcggcg agatcatcgg
    12841 aatggctacc gaactggtgc gatgtgatga tccggcaatg gcgcgtctgc ttttgagcga
    12901 tgtggtgatc gtcgaaaatt ttgaaaaagc aacgcggctt gccgatcaat ttcccgctgt
    12961 acgtttcgtc acattgcaag gtgaaatcgt caaaggcaat tatctcatca aaggcggcgg
    13021 taccgtcaaa tcatccgata gccttatcgg tcaacgcgaa gctttgaata aactgcagga
    13081 tcagttatcc caatacgaag cctccgttcg ccgttatcag gacgaactgc agactaccga
    13141 cactacgctg cgcgaactca cccagacgat cgcaaataat caaaatcttc tgaagaatac
    13201 tgagcaaaaa tttgtcgctc tggaaaaaga aatagcccaa tccgaatacg aacaaaaacg
    13261 ttcccgtgaa tccttgacac gcaatgaaga tgcgcttctg caagcacaac gcgagatgga
    13321 agcgtttcag cttaccatcg aagatatggg ccctgaaatg gatgagctcg aaggtcggcg
    13381 cggttcgctc gaacttgaaa cccggcgcat ggaacagcag ctcgccgaac tggaaaagca
    13441 gcggcgcgaa cgttccgaag gattcaacga aatgatttca tcctttctcc gtatggaagg
    13501 agaaatcaaa acaaaccgca acaatatcga atccagccgc acacaaattg cagatattga
    13561 agcaaccatt cttcgtcgcg aagaagaaac tgtcgccgct gaaaatgaaa ttgcccgtat
    13621 gggtgacgtt ttcaccgaac gcgaaggcga actcatcact ctttcgcgtg aacgcgacgt
    13681 gatcgaacgc aaccgcgatg ccgaaggtac cgaagtcgcc cgtctgcgtg aaacgaccgg
    13741 aaaaatagaa gccgagttga aaaaaatgcg ccgtcagcgt gaggaattcc tcaactcacg
    13801 ccatactatg gagcaagaaa cgaccaatct tcgttttgaa cttcgtgcgc tggaagaacg
    13861 catcaaacgc gatcacgatt ttgacttgac caaagactcc atcgaaacac tgatcctcag
    13921 tacggaaccc gggagtaaag aagaggaatc cgaagaagat gcaccggaga gcgctccgga
    13981 taccgcgccg caggaagtat ctggcgtcga aaatcaggaa atgaatattg atgaagtact
    14041 ggaaagcgat ctcgtttccg atgcatcgca agcacctgag acggcatctt ttgatccgga
    14101 ggcgactaaa ctcgaaatag atgaactgcg ccgtaaactc aagagtatcg gcccagttaa
    14161 catggaagcc tttgcggaat ttcaaacgga aaaagaacgc cttgatatat tgctcaaaca
    14221 gcgtcaagat ttgctggacg ccgaagcgca acttttggaa acgatcaatg ccatcaatga
    14281 acaggcacaa aaacagttca tggaagtctt tgaaaaaatc cgtgccaatt ttatcaaaat
    14341 atttacgggt ttgtttgttg attccgaagc cgatctcaag ctggaagcca atgaagatat
    14401 tctggaagcc aatattgata ttttagcccg accgaccggc aaaaaaatcc aacacattca
    14461 gctgctctcc ggcggtgaaa aaacattgac ggctatttct ttgctttttg ccatctacct
    14521 tgtcaaaccc agtccattct gtattttgga tgaagtggat gcgccgctgg atgacaccaa
    14581 tattgataaa ttcactaaga tcctcaaaga cttctccaaa gacacccagt ttatcgttgt
    14641 aacgcacaat aagcgtacga tggaagcggc gcgtaatatt tttggtatca ccatgcagga
    14701 agccggcgta tccaaagtcg tatcggttaa attttccaat cgcggcgatg tcagcaaaac
    14761 tgacgatatc gagcagttta tcaacgaaaa tcaggtcgaa gacctgacga aagaagtcaa
    14821 agacacaccg ccgtcgccga gcgttgcgtg ataccgtaaa agtacaccgt ccacccattc
    14881 accgatccgt cgcaatctac gaatttgcgc aataatttta cgcattactg cgttctgatc
    14941 atatttttta tctcgcctgt acttattcct gctccttgtt aagctttgtt actatttttg
    15001 aaattcatcg cacaacttat catatcaatt ttttattggc taacaccgtg cgaacatctt
    15061 atattttcaa aataccggct atcgtattcg gctctcacta tggaatctat agactttaaa
    15121 aaacagcgtg cacagcgtat cgtgcgttcg cttgcccgct cgtatcctga cgcacgatgc
    15181 cgactgacct atcatgacgc gtttgagctg cttatcaaaa ctatcctctc tgcccaatgc
    15241 accgatgagc gtgtcaattt tgtcggcgaa acacttttcc gaaaataccg ttctcctgaa
    15301 gattttattc aggttccaca ggccgaactt gaaaacgata tccgctccat cggttttttt
    15361 cggagtaaag cgcggcacat tcaaacgtgt tgccgcacgc tcatacaaaa tttcaacgga
    15421 cgcgttccga gtactatgga tgaactgatc accttatccg gcgtaggacg aaaaacagct
    15481 aatgttattt tgggaaatat ttataatatt ccatccttgg ccgtggatac acacgttata
    15541 aggatcagcc ggttgcttaa actaacgcgg cacacggagg ccgaatccat tgaagccgat
    15601 cttatgaaaa ttctttcgcc taaagattgg gtcatgtttt cacaccgcgt tcaaatgcac
    15661 ggtcgaacgg tgtgtattgc ccgtcgccct cagtgcgatc attgttcctt agctaaagat
    15721 tgcccgtccg caaataaata aaaaaatgaa ccttacgcgc cgtctcctga tcattgcctt
    15781 ttttttcagc gctatctcgg catttggcca acgcctcttc gttcgccatt attcgaccgc
    15841 cgaagggctg cctcaaaatc aggtttttgc cattcatcag gatcaacatg gttttctttg
    15901 gtttggaacc ggcggcggcc tcagccgata cgacgggcgt caatttcgca attacaccaa
    15961 agaacatggc ctagccagta atgtcatccg cgccattcat cgcgacaaag atcaaaacct
    16021 ttggttggct accgatgaag gtatcagttg ttatgtagct caacgcgaca catttatcag
    16081 ttatcattcc gatcatatcg gacaaggtac cgtgcgcgct atagccggag acgatcgtcg
    16141 gttatggttt gctacggccg gtgggcttac gcgttatgat tttaacgatc attcgtttac
    16201 aaactttacc acggccaacg gtttacctgc caatctcgtt caagccgtta ctgtaacagc
    16261 ctacggaaaa gttttcatcg gaaccaatca gggactggct gtgatgtccg atccactcag
    16321 tgatcgtatc gaacaagttc caatccccgg aaataacaaa ttacttctcc gtgtcgaagc
    16381 gctttattgt gatgcgcgca accgcatttt tgccggaacg caaggcggcg tcatacgcat
    16441 tgacagtctc aaaccggaaa tcatcagcac agcggacggt ctgaccaatc ctgtcatccg
    16501 aggtattaca agcgaccaat ccggtcacat ctggtttgct accgaagacg ggcttcatgt
    16561 catgacccga tcagagcgcc cttacttatt taacagctac accactcgca atggtttttt
    16621 tgccaatcaa tattacgctg tttttcagga ttccgaaaac aacctttggt ttggctcctc
    16681 ccgcggtgtc agtaaactga tttccgctga cctcatttca tacaccgaag cagaaggtct
    16741 tggcaacgat gtcgtgatcg gcataacaaa ggctacggat aaaacatggt atattactac
    16801 caacgacggc atttccattt ttcgcgaagg acggtttcaa aatctgtcag atacagaagg
    16861 tctcattgcc aaagaaatat gggatgccgc aacggattcg gcgggagtaa tatggttagc
    16921 gacctatcgc ggtcttcagg ctttgcttcc caaagaaccg cgcacgcggc ttccgataca
    16981 taccaaactt ccgcgcgcag gcattgtgtc atccggatat tacaatctta ccttaccacc
    17041 taacgatttt atacataata accgcatcgt ggacgttttg gttgaccatc gcggacgtat
    17101 ttggtttgcc agcacggata aaggaacggg ttacgttgaa atcaattcga ccggtgaaat
    17161 ttccgttcga acatttacta ccgcagacgg tttgtacagt aacaacgtat ggatgatcta
    17221 tgaggaccgg cgcgggcaaa tatgggcggg cacgatcggc ggcggtatag cgcgttggga
    17281 tgaggtatct cagcgtttta ccagcctcaa caaaggtcgc gggctttcgg atgatgtcgc
    17341 attaagtatt tacgaagacc gacgcggcgg tttatggatc ggtactgagc gcggcgcgat
    17401 gcgtttggcg ccggaaatcg tgagcggaga ttcttttccc ggcgatctgc gttcttacat
    17461 tagattggtg tctaaacgcg acggtttgtc cgataatacc atcaatgcga ttgtggaaga
    17521 ttcggaaggc atgatttggc tgggcacgaa taacgggctt aatcaattta atcccgaaac
    17581 cgggctgacg cttcaagttt acaacaaaaa gcgaggcctt attgacaatg aagtaagtac
    17641 acaaaacgca ctgcgctttt atgatcacga gaccttgatc gtcggtacca gtgccggtat
    17701 cacgttgctg ccattacgtt catttcgctc gcccctaccg acccgacctg ttgttttgat
    17761 gcaagaattt atggccgaag ataatgccaa caaaacagcc cggttgatcc ctattgctat
    17821 gcgccccgat acaaacgttt cggatgcatt tttcagtaca cttcgtacat ccgtaccggt
    17881 ggtcagctat ctcgaaaaca acatcacatt tcgttttgta gcgccatcgt ttaaagatga
    17941 aaatgatgtg cggtaccgtt accgtttacg cggctttgac cgattttggt cggacccgac
    18001 accggagaat catgtgcgtt ataccaacct caacgacggt gattacgtat ttgaggttat
    18061 cgccggcaac ggcaatggcg cctggagcga cgttcccacc cgcgtaacgt tttccgtcat
    18121 gacgccccta tggaaaagct ggtggttcat cactttttca atcacatttt tcagtttaag
    18181 catctacacc gcttataagt tccgcgccaa tatcgcacgc caacgcaccg ccgaacttga
    18241 acaaaaagta ttgcagcgta cacgcgaatt ggtcgtacag aaagagaccg tcgagcgtat
    18301 tttgaacgaa ctcaaagaaa cgcagatcca tctgatacac tccgaaaaaa tggcttcgct
    18361 tggtcagatg gtagccggca ttgcgcatga gatcaataat ccggtcgctt tcgtcaaagg
    18421 aaatatttca ttattagagc ggcgtcttca tgatctggaa cgcgtattta ccagtttctc
    18481 cgatgttttt gatttctacg ataaattcaa cgccttcagc gacgatctac acaaagattt
    18541 tcgcgccaaa ctggaagaaa tagatcgtat tattgcgtcg acgcgatttg aaaaattttt
    18601 aggcgatctg cctcaagttg tcaacgaaat gcgggacgga gtggatcgca cgcaaaaaat
    18661 cgtagaagat ctgcgaaatt tttcacgcct ggacgaatcg caattcaaag aaatttccat
    18721 cattgatagc attgaatcca cactcaacat actcaaaaac gagtataaaa cgcgtattac
    18781 cattcatcgc aatttcggag acgtgccacc ggtgtattgc aatccgggac atatcaatca
    18841 agtttttatg aatttgctgc aaaatgcttt tcaagcgatc gaaggtgacg gcaacgtttg
    18901 gattcgcgtt gttgccgcca gcaataatat tttgatcgct attcgcgacg acggtaaagg
    18961 cattcccgga gaaattcaaa accgcgtttt tgatcctttt ttcacaacaa aaccggtcgg
    19021 taaaggtacg gggctcggtc tggctatttc atacaaaatc atcgaagcgc acaaaggcac
    19081 gatttttttt gagagcaccc cgcagcaggg aactgaattt aaaataacat taccgatacg
    19141 ccgcttagcc gatctccaaa agccggaata agcttgtatc aaaataagag atgtattatc
    19201 tttttttcga ttttaacttt ctttttaacc acatcaactt accgccactc caaacgttct
    19261 aaggagtcta tatgattaaa aaagcttcat tatcttacga tgataaagtg ctggatttac
    19321 cgctgatcac cggcactgaa aacgaacaag ccgtggacat cggcaaactg cgcggcgcta
    19381 cgggacttgt caccatggac ccgggttacg gtaataccgg cgcatgccat tccggtatca
    19441 cgtttcttga cggcgaaaaa ggtattcttc gttaccgcgg atataatatt caggatttgt
    19501 gcgaaagttc caatttccaa gaagtcagct acctgttgat tcatggcgaa ctgccgtcgg
    19561 cacaacaact tgaagaattt aaacgcggta tcacgcgcca taccatgctg cacgagtcgc
    19621 tgcgcaagct ttacgatgcc tatccgcggg atgcccatcc gatggcgctt ttgtcttcca
    19681 tcgtgtgttc gctatctacg ttttatcagg attccattga tccgttgaat aaaaaacatg
    19741 tggaaatttc gattttccgt ttgcttgcca aaattcccac gatagcggcc tatgcttaca
    19801 aaaaatccat cggtcagcct ttcatgtatc cgaataattc gctcagttac tgtgccaatt
    19861 ttctgaatat gatgtttgcg gttcctgccg aaccgtatga agtggatccg gaaatcgaaa
    19921 aggcgcttga tctgcttttt attttacatg ccgatcacga acaaaactgt tcaacgtcaa
    19981 ctgtacgtat ggtaggcagc agccaagcca atctttttgc ggctatttca gccggcattt
    20041 gtgcactgtg gggcccgctt cacggcggcg ccaatcaaga ggtgcttgag atgctgcagg
    20101 aaattcacaa cgacggcggt gatgtcaaaa aatatgtgga aaaagctaaa gataaaaatt
    20161 ccacattccg tttgatgggg ttcggtcatc gtgtatataa gaacttcgac ccgcgcgcaa
    20221 cgatcatcaa aaaaacctgc gaccgtgtat tgtcgaaact tggtgtaaat gatcctttgc
    20281 tcgacatcgc gcaccaactc gaagaagccg ctttaaaaga tccgtatttc atcgaaaaga
    20341 aattgtaccc caacgtagac ttctacagcg gtattattta caaagccatg gggttccctg
    20401 tcgaaatgtt tacggtgctt tttgccatcg gacgtatgcc cggatggatc gctcaatgga
    20461 aagaaatgat ggaatccaaa gaaacgcgca tcggacgccc gcgccagatc tataccggat
    20521 cggcagagcg cccttatacg ccgattcaca aacgcggata acctttttcc ctgaaactaa
    20581 aaaccggcat aatttcatgc cggttttttt tatcttcagt ttgaagaaat tagataaatt
    20641 gaacatattg cgtcacgatc ttcgctcgca caacaacttc gttttatttt cttttactat
    20701 aggaaaaatc atgcgtatcg tagcgcagcg tgttaacaaa gcccatgtat cggtagatgg
    20761 ccgcatcacc ggtgcaatcg aaaaaggcct cgttttactc atcggcatta aaaacggcga
    20821 cacatcggct gatgtaaaat tttttgcgga aaaatgcctc cacctccgca tcttcgaaga
    20881 tgccgaagga aaaatgaatc gcagtattat agacgccaaa ggctctatac tcgcagtttc
    20941 tcaatttacg ctctatgccg atacaaccaa aggacgacgg cctaattttt tggaagccgc
    21001 gaggccggaa ttagccgaac cgctgtataa tgaatttgtc acgcacttac gatcgcacaa
    21061 tatacacgtt gaaaccggca tttttggcgc catgatggaa gtggaaattc acaactccgg
    21121 cccggtcacg attattttgg aataaaaact acgcatgaat ttttttccac gcatctcact
    21181 attgtatttc catctttcac acttgatcgt attgagcttt ttcacaacgc ttgcacaaac
    21241 ggtttccgat tcttcgttta ttgctacaca gaaagactct tcccaaactc ccggtaacgg
    21301 cgtggatacg atgatctact acggcgctga tactacggag ggcacactga ataattcaac
    21361 cgtggttctc aaaaacaatg catgggtccg gtttaaaggg attgaaatca aagccgctaa
    21421 aattaccatt gatcagcccc gcaagatcat gatcgccgag gctgtacccg actccgtcga
    21481 ttcactcggc caagtggcgc gctatcgcgg tatacccgaa ttcaccgaat ccggccagat
    21541 gtttcacggc aatgtgatgg agtacaattt tgaaaccaag cgcggtaaag tcgtcatggg
    21601 tgaaaccaaa atgcaggatg gtttttatta cggctctacc atacgaaaaa tcggcgacag
    21661 tacattatat atacgcggcg gacgatttac gacatgcgat gagaaggaac cgcatttttt
    21721 cttccgcacc aaagaaatga aacttactgt gcgcgataaa gtcgtcgcca aaccggtgat
    21781 actatacata catgaagtgc cgattttcgc cattccgttt ggtgtatttc ccagtaaagg
    21841 cggacgcgct tccggtataa ctcctcccgt ttattttgag acacccggcg gcgagagaca
    21901 aattcgcaat ttcggttatt attgggcacc caatgactat tttgatgcgc tcctgcaagc
    21961 cgattattta gacaaatccg gttatcagct tcatggcggt ggcaattacg ccaaacgtta
    22021 tcattattcc ggacgaatgg atttttctta ttctcaattg cattcactta gcacgacaag
    22081 ttataacgat aaccgaacaa ccgccggggc actcagcctc acccatgcgc atactttgag
    22141 cgaacgatcc aatctcaacg ccgacattcg ctaccagagc agtaaaaatt attttcaaaa
    22201 taccagtatc aaccaacagc aaatattaaa tcgtcagcaa aattcgaata tcagttacaa
    22261 taatacattc gactggggtg gtatatcggc cagcgcatcg cattctcgta atttggacaa
    22321 cggtcaaagt tcgcttacat ttcccaatgc gacgatcagc aagtcctcat cctccgtgat
    22381 acccaaatcc gaagaacgtc gcaacgagcc ggatcgctgg tatcacacga ttcgttattc
    22441 gtataatgcc aacctactcg gacgacgtga aatcgccaat gatacttcag acgtgcaaaa
    22501 cgggtacggt ctacggcaca atgcaggatt caatgcgcca ttcaaagtgt tttcgtattt
    22561 tacattcaac cccaatatca atgtcgtcga gacgtggtac gatcgccgtc atgagaattt
    22621 cggctatcgc gccaatggtc aggacaccag cgtcgtcaaa cgcggatttt atgccacgca
    22681 cacgtatagc atgggcgcat cgctttcgac gaaagtttac ggtacgagca atcccaatct
    22741 gttggggtta aaaacttttc gtcatgtcat gactccgagt gtgagtatta attacacgcc
    22801 tgatttttcc gaatcaaaat ggggatatta tgaatcggtt caagatacgt cggggaaaac
    22861 cgtacgtaaa gaccgctacg gaaaaaacct gacgtttggc ggaacacctt ccggacgcca
    22921 gctctccatg ggcttcgggc tcgcgcatac atttcaagct aaagtcaaaa cttccaaaaa
    22981 ggacagcacg caacgcgatc ctgatgtaag aaaaatagac attatgaata tcaataatgg
    23041 ccttagctac aattttgaag ccgaagaatt taaactgtct aatctgacta cttctatcgc
    23101 ggtagccaat gatttggcaa aaaacgtaag tttgtccatg aatctcaccc atgattttta
    23161 caaattcgat acgacactca accgtcgtat taataaattc agaaactttc cgcgcttgat
    23221 caatgcgggt atcaccggag gatttagcat cgaaggcggt tccggcgcat cttcaacaac
    23281 atcgggttct acaccttcta caacggcctc gactacaccg caaggcacgg tgacacccaa
    23341 ctacgggcaa tcctacttac cgtcacaacg cgagctcccg agcggcgttc cgtggaatgc
    23401 acgttttaat tttaattatg atattggacg acttgatccg cgcaatgtca ctaaaacatt
    23461 tagtggcaat atcagtgcag gtacgcgcat tacccaaaac tggcaagtca actactccgc
    23521 tcagctcaac ttactcaaag agaaaatcgt cagtcagagt ttctcacttg ttcgtgatct
    23581 tcattgctgg acgatgcgtt ttgattggac acctaccggc ccggccgcag gttatttttt
    23641 cgtcattcag gtcaaatcta cgcagctcca ggatatcaaa ctgcaacgca ctgactatgg
    23701 caatcgcatt ttccaatagc gattgaaaaa atcgtgcttt agaaaaaata agaaacttgt
    23761 ctcgttgtaa ttcgtaaaca acttaatctt attctcatca ttatccatgt cggaggagtt
    23821 atgaatgttt tgaacagcac ttttttgctg cgccttgccg tttcgattac gttattcatg
    23881 cacagcgtac cgggcatgtt taacggcggc gtcaataatt tcggtacttt ttacctcaac
    23941 gaagtcgggt ttgcacctct aggtgttccg ctggcgtggg ctatcaagct gtcgcatgta
    24001 gcggcaattt tttgcctgat gtttgagaaa tacgtaaagt tagcttcgct tgtaacaatc
    24061 tttgtcttgc tcgccggtat cgtgatggta cactatccgg atggctggta tgtggttggc
    24121 ggcggacgta acggaatgga atttaatgta cttctaattt ttgttttact ggctattatg
    24181 tttcccaatg ggctaaagaa aaattgacgt atacttttta atcttccttg taaaaaacgc
    24241 tgcatggttt gtgcagcgtt tttgttttta atgttttcga ttttacttct tcagatacgg
    24301 tttcagatgt tttccggtat atgaattccc aacctgtatt acttcttcgg gtgtgccggt
    24361 tgcaacgatc ataccgcctt tatcgccgcc ctccggaccg agatcaatga tgtagtcggc
    24421 acacttgatt acatcaagat tgtgttcgat gacgatgatg gaatgaccgg cttcgatcag
    24481 tttgttaaaa ctcgttaaca gtttattgat atcttcaaaa tgcaaaccgg ttgtcggttc
    24541 atccataata tacagcacgt gattgcccga tttgtccgcc aaatgagccg ccaatttgac
    24601 gcgttgcgct tcgccgccgg acaaggtcgt tcccgcctga ccgagtttga gataccccaa
    24661 gccgacgtca tccaacacct gaagtttttt tacaatttta ggatgcgctt taaaaaacgc
    24721 caacgcttcg gtgatgggca tttccaacac atcatggata ttcttatcgt gaaaatgtac
    24781 ttctaaaact tcttttttat aacgcttacc cttacactct tcgcattcga gatacacgtc
    24841 ggcgaggaac tgcatttcca ccaaaatttg gcccgcacct tcacagactt cgcatcgccc
    24901 tcccggcaca ttaaacgaga aggtgcccga ctgataaccg cgcattttgg cggtcggcgt
    24961 ggctgcaaat acatcacgaa tcagatcaaa tactttgacg tatgtcgccg gattgctgcg
    25021 cggagtgcgc ccgatcggct gctgatccac catttcgatc gcatccacat gctccacacc
    25081 gagtatttct ttgtgcaatc cgattttatt ggaccattga cctttttttc gcatagccgc
    25141 agcatacaaa atgtcatgta tcagtgtgct tttaccggaa ccgctgacgc cggtgatgca
    25201 cacaaacatg cccagcggaa actgaacatc aattcgtttg agattatttt ctgcggcacc
    25261 tttaagaacg atagattgac ctctaccttg acgccgttcg gacggaagag aaatttcttt
    25321 tcgaccggag agatacaatc ctgtcagcga cgaagcgctt tgacaaatcg ccgcatgatc
    25381 gccctgaaac accacttctc cgccgtacct acccgcacga ggacccatat caataatttc
    25441 gtggcaagaa gccatcatat cccggtcatg ttccacgacg acgacggtgt tgcctatcgc
    25501 ctgaaggttt ttaagtatat gaatgagttt atcattatcg cgcggatgca aaccaatact
    25561 gggttcatcc aaaatataca aggagccgac caacgccgaa ccaagcgatg tcgccaaact
    25621 aatacgttgg gcttcaccgc cggagagtgt cccggccaga cgattgcatg tgaggtattc
    25681 gagtccgaca tttttcagat agttataacg tttgagaatt tcttccagcg gacgacgtgc
    25741 aatctcacga tccatctccg ataattgaat gtttacaaaa aaatgcacgg cttcaccgac
    25801 cgtcatatca ttgatttcgt gaatcgtttt atcggcgact ttcacacaat tggcatctgg
    25861 acgtaaccgc gagcctccac aatccgagca cgttacataa ccacggtatt tgctgagcaa
    25921 aacgcggacg tgcattttgt aagttttgct ttcgagccat ttaaaatatt cacgaattcc
    25981 gtcaaatccg tctccgcctt gcatgacttt ttcgtattgc tctttggtaa gatccatgac
    26041 cggaatgttc agcggaatgc cgtttttctt agccgctttt tccaatcgtt cctgaaaatc
    26101 ggcgtaaccg tccgtcgtcc acggtttgat cgcaccttcc ttaagtgttt tggttcgatc
    26161 cggtatgaca agatccatat ctacttcgat cttattacca aaaccctgac atgacgggca
    26221 agcgccgtaa ggattattga acgagaataa acgcggatct ggttcgatat atgccgtgcc
    26281 gcacgcattg cattcaaact tattggagaa acgaagcgtt tcgccttcca ttatttgaac
    26341 gatcattttg ccttcaccgt tgaggaaagc tgtttcgacc gaatcggcaa cacgcgcttt
    26401 ttcttctttg tttacagcga tacgatctac gagtacacga actatttttt gtttcaggat
    26461 tttttttacg acggcttcgt cattgagttc agcgataaca tcatccacga tcaagcgcgt
    26521 aaagcccttt tcgtgaagcg acaaaatcgt ttgttcgtta gctgccagtt caaaaagtat
    26581 atgcaattta gttcgctccg gcaaagcgag aatccgttgt acgacataac cgacggtatc
    26641 ttttcgaacc aatgcaccgc aatttcggca gtatgttttt cctgcacgac caaagagcaa
    26701 acggagaaaa tcataaattt ccgtaaccgt ccccaccgtt gagcgcggat ttttggccaa
    26761 cgtgcgctgt tcgatggcca acgcgggact gatacctttg atatgatcca cttccggttt
    26821 gtccatgcgc tccagaaatt ggcgcgcata cgccgatagt gattcgacat accgccgttg
    26881 gccttccgca tacagcgtat caaacgccaa tgaagatttg ccggaaccgc tgacgccggt
    26941 gatcacgacg aatttgtttc gtggaatatt gaggctgata tttttgagat tgtgtacgcg
    27001 tgcaccgcgt atttcgatca tgggttccat atacttacga tttgataaat ggctttaatc
    27061 cgaatttcga agcgatcaat gtttgtacga cttgttcgag cgtcacttga ttaacatcgc
    27121 ggccgttacg aatcacggta tgtttttcac cgtaaggtcc gatatgccgc tcatctttgg
    27181 gtccaaaaat tgcaaacacc ggtgttccta cggcggtcga aagatgcata attccggaat
    27241 cgttagaaac aaaaaaatgc gcttgcgcga tcaacgccgc ggtctcttcc aaagtacact
    27301 gtcctgcaaa attgagcggc ttatgctgtg cctgctccat aatttttcgc acatcatccg
    27361 cttcatcttt accgccgacg ataaaaacag ttgcatcgta tgtagtatat aaataatccg
    27421 ccaaagcggc ataacgttcc gacggccaac gtttgaaagc tgcagcaggg ttgcatccgg
    27481 gatgaaaacc aatgataagt tttttcctgt catgcgctat tcttgtttcc attttggcta
    27541 gcgtaacatc gctgatatga aattttggcg attgttttga ccagtgatta acttttaaaa
    27601 aatgaagcag ttcaagattg cattccaacc gatgaacgcc ttcaccgatc ggggtaagcg
    27661 catgatacaa ggcatctaag tttctaggaa atttatgcgg tgagttatgt ttgattcgaa
    27721 ccggtatacc cgctaaaaca ggaagtaaaa gttgcgagta ggtgttcgac ggaatagcgc
    27781 cgatatacag atcaaacttt tctttgcgta ttttcccaac caacccgaaa tattttttta
    27841 tcgacgaaac atccatcgtc cagatttttt gaatacccgg atgcgaacga aacacatgct
    27901 gtgtagcggc gctacagaga acctctatgt gagactgcgg aaagttattt tgaatttcgc
    27961 acaacaacgg ctgcgccatc agcgcatcac cgataccggc ggaaatacta aggagaattt
    28021 tttccattta ttataaaaaa gccataggtt acattttctt ataatatgta tcataccaac
    28081 gcgccaaaaa ccaaagactc caaatgcgtg aagcatggtt tttggaactg tttaaatggg
    28141 tcgtaatcat ggcctctata aaatgaaggt tcatgtgttc tttgatcagc tcacactctt
    28201 ttatttcgtg atgaatcata cgggttaaat cgggattaga tttgaaccag tcacgcatag
    28261 gcaatgaaaa gccgcgtttg gttcgataaa ttagttcttt agggaatcga ggctccaata
    28321 ttttttttag tacatatttt ttttcacttt tacgcattct catcgaaacc ggcatttgcg
    28381 ccgcaagttc tataaccttg tgatcaagca gaggaactct gacttcaata gaatgtgcca
    28441 tgctggctct atccactttt gtgagaccat tttcaggtaa ataggtatgt atatctatat
    28501 tttgcatttt attcaaaagc gcatcggagc tattcatata agagtcgaat aatcgttccc
    28561 gatgttctac agagaatttc atcatatctg cgatttggtc cgaatgaaaa atattaatca
    28621 taccgtaata acgaatcata tctgattgcg aatggtatcg aagataattt tttccaggta
    28681 tatttcgcgg ccaaaatgtg gctatttttg ataaccaaac tttatttaac catggatttg
    28741 attgggacca ttccaaaagt tgttcgtatc gtttataacc gccaaatact tcgtcaccac
    28801 catcgcccga taaagctacg gtcacatgag atcgaattat ttttgcgatt tgatatgttg
    28861 gtacagcgga gctgtctgca aaaggctcat cgtaagcata tactaaattc gataaaatcg
    28921 tattgagatc tgccgcttgc acactctttt caacatgtcg gctttgtatg atagcagaca
    28981 cctgacgagc gtatgcgctt tcgtcaaacc tgctttcgtt catactgacc gtaaacgtat
    29041 tgatagtgcc aacgtgtttc gccataaaat gagacactat agtggaatca atacccccgc
    29101 ttaaaaaagc tccgacaggc acatcgctga tcatatgtat gcggacagaa tctgaaacaa
    29161 cggattcagt tttatcgatc cacgttgtat cattgtgtgc tccatctgta tcttgaccaa
    29221 actgaatgtc ccagtagcat ttgcttttca tattatacct gcccccatca aacgtaatat
    29281 gaagccagtg agccggaggg agtttcttta ttttattata aatagaagca ggagaaggta
    29341 cataaccata ttgcaggtat tcacgcatcg ctgaatagtc aatagttcga tctatacttt
    29401 tatcctgaag aatagctttt atctctgaac caaataaaaa cttattctga tctgcgtaat
    29461 aataaacggg tttaattcca actcgatctc ttgccaaaaa aagcgatttg gtacgttcat
    29521 cccaaatggc taatgcgaac attccacgga atctgtctaa gcaacttgcc ccccactgta
    29581 gataagcttt aagaataact tctgtgtcgc tttgggtacg aaagttccaa cctaatttta
    29641 ttagttcctc cctaacttcg ccgaaattat atgtttcacc gttatatact atgcaagact
    29701 tgccgtcgtc atcatacatc ggttgttgac ctgtttttag atcaatgata cttaaacgtc
    29761 gatgcgctaa tccgacatgt tgacgaataa aaaaaccatc gccatcaggg ccacgatgag
    29821 ccaaggcgtc acccatcgcc ttaagtaaag attcattaac gggctcacca gtaaaattca
    29881 taacacctgc aataccacac ataaatgtaa cctcaaattt tattattcga aaagttccga
    29941 ataaactctc aatgagttct tataaaaccg tttaacattg tacttttcaa ccactagacg
    30001 gtgaccgttc tcacccaatc gctgtgtcaa aatcggatca gctcgtaatt gttcaacaat
    30061 tcccggcatt tcagatggag tattaataat ataaccgcag ttttgacctg atataatctc
    30121 tgcagggccg ccactatcgg aaaaaacaac tacaggtttc tttttataca tagcctcaac
    30181 caatgataca ccaaacgttt cgttatcaga ggataataca aacagattca tcgccgacat
    30241 tgcatcagat gaattttcca gaatacctgt gaatgatact ttatctagca ggttattgtg
    30301 cgcgacaaat tgtttgaggg cttgttctat aactgtatca ccgccgccaa cgacaactaa
    30361 gtggaaatct tttaaattcg aaatcatgga aaatcccatg agaagcctat ctaagcgttt
    30421 gcgagtgtca aaccgacata cagttcctat caaaaaaaca tttttatcta tattgtatga
    30481 tgcacgaaaa tctgtttcat cgccttttct attattgaat tcgataccat tcattactat
    30541 gcgtattttc tggggttgaa tgccatattt ctcaacagca cttttagcgg tatagcggct
    30601 attagcgatt aaataagtac accacttgcg gagaaatagc tcgaaatatt ttcgtttaat
    30661 aagtctgtat aatgaacgtc tgttaggaat ttttgattgg gaagtattct cctttgccgg
    30721 taattttatt aattgatcgc ccctacggtc atcctcgcta tgtcgtctcc atattgtttt
    30781 ttttattttt ttcgctaaaa tgaagaatac atcgcccaaa cttttcttgt taagtaaata
    30841 aattcctgat aaacgatata aaatagattt ccctgataaa atacccgcca agaagaatct
    30901 ccaatctaca aagtggaaaa ctattatgtc ctgcttttcc attaaagaaa taatatctcg
    30961 taagcgagtt atgttataat aaacagaaat ccccagcgat tttatttttg tataataagg
    31021 accaacggga tatttgaaaa aagccgacgt ctgaatttgt ttatcttttg attcttcatt
    31081 taatagtaaa taaagaactt tctctcgtcc gccgatattc ccattgctta tgatatgaag
    31141 aattttcatg tatctctttc gtaataccga gtagaatcgc ataaaccgat ggaatggata
    31201 gaataataac gatttatgta atctaaaatg cgctttattg tgtttattgt ttgccctacc
    31261 tctaatggtg attgtgcata tgggctagcg ccgggatgta gttcattact gtggaacatc
    31321 atattcaata ctataggttc cttagtagta tgaattttta tatgatgttt aatcagattg
    31381 atcaagctct cagcattgtc tgttctcggt cttaatgaat acgttttttc cttttctttt
    31441 gtaagaagaa tacgaaaaag gcttcttatt cggttttgct ccgacagagg ttttccaata
    31501 cagggcggaa tgcgttgaaa aaaccaatta tgaacggtca ttggtacatg caatatttta
    31561 cgaatctctt gtttttcaaa attcatccaa ttttgcaaat caggataaaa aggtttaacc
    31621 ggagtatagt aaaaattgta cgtagcattc tttgttttaa ttaattgaaa tggaagtaaa
    31681 ctcgaatcaa cagcataacc aagctcggcc aaaatacgcc atgtgtttgg tccgatgcca
    31741 aaccttccag cgcggaaaga tttcggcata tagccgaatt tatttttaaa taattccgta
    31801 agggattcaa gcttttcccg ctctatacct tcatcatagt cacattgaaa atcgttagtc
    31861 catcttggat tttcaagttt atcatagcct atgagttcac catgcaaatg cgtacctaat
    31921 tcacatctat cttgaattga tttaaaatag ttcacacttt cttgattata gattacctca
    31981 gggctcaata gataagttgg ttttacttta tatatatcaa atagtggctg aagtttttca
    32041 gctacgcctt catggatatt acgaaacgag aatggatacg taatactcca atcaatactt
    32101 ttatcacact cggtatcaat tgatactata aaaaaaactg tccttctcat gtgatcattt
    32161 caaaaaacgg gcgactttgt aagttagatg ttatattttt tgcattaaaa tttttaaaac
    32221 aattcgaaac tgaaaactaa taaaaaatac aacagcgccg taaaaagaaa gtgtcagcca
    32281 acgatttctc acacgattta tatgcgttaa gataatttga aaaccagcca gcatagcaaa
    32341 agaaaaaaaa acatgatata aagtaagaac tctaggtata ataaagaaaa ataagtaaaa
    32401 acatgtaaaa ccaatgctca acacaatgaa tcttcgatag attgaattcc aaaaaattac
    32461 acatgctaat aagaaacaac ctcctatcca cgaataagta gaaacaaaat agttagaaaa
    32521 acgttttata ttatatggta cgcgttcaag tattttgtag ccaaggttaa tcaaatacac
    32581 cgaagtcccc cccgcctctt cagcgttttc ccatactcga cttatgacgg catatcgagt
    32641 atcataattc gtaggatttt gatatgggct attcagttcg ccaaggacaa gattgacgta
    32701 tgttttaccg cttatttgcc aggcgccggt ctgattttta agaaaaacac catacggtag
    32761 atagagtacg ataaaacaga ttgcagcgat ccctttgatc atacgactat taaaaacgct
    32821 caatgaaatt ataactagcc ccagcaaaac tccttccggc ttagttagcg acaataaccc
    32881 ccataacatt cctaaaacaa ttccccagct aagattcttt gtattctttt tgataattga
    32941 tacccatata agcgataaga tcagcaagat cataagcaaa tctgaataac ctcttccggc
    33001 ctgaattaat agttgggaat taaatgcaaa aaaaaacaaa ccgataatac ggagatgaaa
    33061 attaaaaccc attaattgta atattttttt catcaagatt atggataaaa taaatgaaac
    33121 aatgccgata ccaagcagta taggccattc tacaggaatc aaaaaataca gaaggcctat
    33181 aataacggga taccctggcg gtaaaataac atcatagttg tcgttcaaca cgatcccatt
    33241 cccaatgatt aaaccgtgag aaatattaaa atactcccag ggatcattgg aggctataaa
    33301 atcataagga ttactgataa gccatactat tggaactaaa agcattaaca tcaaaatctc
    33361 taaataattt tgtatggaga caagaaaata cttgcgcaag ttttgaagcg aatattcatt
    33421 tatttcggcg tcattgccca tcatcgtttg gtcgctataa aaatcatgtt ttgactgaat
    33481 gggttatatg ggaataattt gaaaaaagtt tcatttgtgc gaaatatcaa acgcgaaata
    33541 atatccggag taatattagg aaggtaaatc aatccatcat ccattataac tttatcaata
    33601 taaaatcctt gcttatcaag agcgtttaaa acttctcgtc tcgaataata ttttggagag
    33661 gatttttttt tccctttcag ataccgcgta acaaattttc tcccaccata tttatttggc
    33721 gttagaatca ttaaacgccc atttgacttt aaaatacgat agcattcttt gagcgtattt
    33781 tccacattta catattctaa caatccgatc gaataaatta tctcaaatga ttcgctttct
    33841 actcttgttt cttgagcatc tccgaggata aaatccgctt tgggataacg ttttttcgcg
    33901 gcattcaaca tattttgtga aacctctaaa cctgaaacct tgcaagtaga ctccaaagca
    33961 aaacctatat aagctccaga accacatccc aaatctagca catctcgttt gtcacacaac
    34021 aaacgaatat gttctacgat aatggggtgc caatgatcat ataagtattt ttcaaataca
    34081 ttacgatttt tccgattatc gtattgatct accgaaatac catcataatg atgtttatca
    34141 ttcattgctg ttctgctttt taaaaggaag tttttttaga attttgtcca agatcattcg
    34201 atcatcttga gatagcccaa aaagaaataa aaatccaaca tacagtaaaa tgaaaacaag
    34261 tatatagatt aaaaaagtaa tttcgtaata aggtaaacga taccagatcg aaatagcgat
    34321 aacgatagaa gacgaaatta acattgcaaa aaaaggtttt aacaataaaa aatcaaacgg
    34381 atgtattttt agtatataaa acgtttcaac agtcgtgagt attgacaata tcgttattga
    34441 taaaaaattt ccgatcgccg caccatacat cccataattc cgaattaaaa tggacataag
    34501 aagcaagttg atgatcgcca ttactatgga gttaatcaca ttgatgcgaa tacgcccagt
    34561 cataagtatg atatttcctg aaaggcctaa aaaatcatga attaaatttc cgatacttag
    34621 aatagctact aactcaaaac cggcagcaaa ttctttacca aaaaccccaa gaatttcttt
    34681 actaaaaagt atgattaacg agcatattgg taagctgagc gttacactcc actttgtagt
    34741 tgttttataa aattggttaa gctcttttat tttacccgca gtatataact ctgccaccat
    34801 tggcccaaaa actgacgaaa tcgaataatg aggtagatag attaatggtt gcaagcgtag
    34861 tgccaatgtg tatatcccaa tctccggcaa agtagcgtaa tatcccaaaa acagaatatc
    34921 aagttggtag gtgtatttac ttaaaagcga attagcaaaa agtgccgaag aaaatttcat
    34981 cattttcttc ttttctattt gaacgggtat tgatttatcc ctgagctcag gagctattcg
    35041 aaaaagataa aatgctgaca ccaaaagtac gacaatatct tggataatat tcgaaccaag
    35101 cgctgcatag aggttaaaat ccaataacaa taacgaggta agacttataa tttttgccgc
    35161 cggaccaagc acatcattcg ccaaaacctt gtactgaaca aattgatatc ccactagtga
    35221 tgatagaaac gcctgatatg atgccgtaag taatatcgag acagcgtaaa attgaaatgc
    35281 ttttgttaca taatttgaga aatattcttc cgaaatagtc tcaggcctgt ggataaataa
    35341 ttcacaaaac aaatcaggga agaatagaat aaaacacgtt gccgctgcgc ttatcacgac
    35401 agatacgcga atagttgtaa atactgaacc tttgatcaac gccattgaac ctgttgcttt
    35461 gtaggtgggt ataaaccgta gattcattcc gtttaaccca atgctggaca aattggcaag
    35521 caaagaaccc cacgactgca ccagagaaaa tattccatac aactcagctc ctagaaaacg
    35581 agttgtaatg acacttgtaa gtggtgtcca aaataaacca aaaatacttc ctatacccga
    35641 tattgcaaat tttttggcta atttagttat atctttttgt cgtgcgttgt ccaaagaacc
    35701 tcaacttaag gattcaccaa tagcgaagga aatttaaatg aagaaaataa aaactctaaa
    35761 tacttggata aataataact aatagtagtc gattcatagt ttcatctaat atagatttaa
    35821 gtatagtatt gtagattctt atattattaa ataagaaaat ataatttttt tttgtcatgc
    35881 ttcttttaat gctagtta
//
LOCUS       DAOQNQ010000006        33106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_425_length_33106_cov_5.978337, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000006 DAOQNQ010000000
VERSION     DAOQNQ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 33106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33106
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_425_length_33106_cov_5.978337"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(347..3544)
                     /locus_tag="PK754_00850"
     CDS             complement(347..3544)
                     /locus_tag="PK754_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB08083.1"
                     /translation="MSITSEKIFRLLFCVFLFLFMLAADTKALDPERSVTQYVHDVWL
                     KEDGLPGISVLSFYQTREGYLWIGSFEGLARFDGAKFTLFNRRSIDAFLSYGLWRILE
                     DKHGYIWMGTNGNGIYRFKDPNFQRLAVTNGLTNDNVLSLAMDQDEMVWAGTRKGLNR
                     IRIISQDSFAVEPVMYRSNVLQDVFGLCTDHDGTVWIGATNTIYRYVEGRLEEFANIG
                     ERIRSMYVDRDNRIWIGTTNAGVFQISENQLRHFTTKNGLPPESIWRVWYDRDGNVWL
                     GSDGMGLFRAKPGSFRDENELISLEQFSEKEGLTNNRISALFEDREGSLWIGTFRGGL
                     NRLRDGKFITYASANGMVHPVVYPIVQEHNGDMWFGTEGGLSHYSGNRFVNYTTANGL
                     SNNQVRGIVRDSRGRLWVGTYGAGLDIFNPASGRFAPAPSAINHAFRNIRDMLKDREG
                     HLWIGTINSIGLIKPDGSHRTFTMQDGLLTTSILCLYEDIYGNVWAGTDGGGAARYDR
                     NKGFFRVLTMKDGLSSDIVFAIHCDRDSVYWFATQSGITRYDPRATDLPFASLTVRQG
                     LPADAAFEILEDNHGYIWLGGAEGIFRISKKDFEDVARGKTNQLTPLLFGRPDGMKTS
                     QTTPSGRAHKTSDGKLWFPTPKGAVVIDPDKIKTNPVPPPVVIERCIADGQSWPIQND
                     VVLPPGILRIEFQYAALSFLAPQRVFHKFKLEGFDTDWMDAGTRRTAFYTGLHPGEYT
                     FKVIACNNDGIWASEPTSFSFTIRPHFYETRWFLLLIFGGMIAAALSLYRWRVRHLMH
                     REKELAALVKLRTHNLEEEKKRTEDARIEAERQREEALRANELKTQFLGMASHDLKNP
                     LAAIISYTDLIIEAYDDKAQSMRDLGYIRKAAHRMLHLINGLLNTVSLESGKVVLQKE
                     KLDVSDLAEQVLFSNQQMLERKRQHVQVQTVPGLYVNADRDRLYEALDNLISNAIKYS
                     DYDKTIRVDLGMNELKIIIRITDEGPGMTTDDMKKLFGKFQRLSAKPTGGETSTGLGL
                     SIVKQIVEMHEGQVWAESESGKGSTFVVELPRFTS"
     gene            complement(3821..3892)
                     /locus_tag="PK754_00855"
     tRNA            complement(3821..3892)
                     /locus_tag="PK754_00855"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3858..3860),aa:Phe,seq:gaa)
     gene            complement(4348..6081)
                     /locus_tag="PK754_00860"
     CDS             complement(4348..6081)
                     /locus_tag="PK754_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB08084.1"
                     /translation="MVDYVQRHLDGVDRLGFYNLLPNNVIRFAVIDFDSHQKPDAYRQ
                     TMDKLSSQCEEYLRKAGLYVYREISKSGYGNYHLWIFFDEDISAKFIRSVLSAFIIQV
                     LKPETALEIFPKQNAGDKGNFIWLPFFGKDVSENKTVFIDASGKKYDAEFKPNSRKTL
                     DELADFYHLELKETTSAKVSRKTDYSEILSNGVPVGERNSTVTRIAGHLKHAGLPISE
                     TKAILQLWNKNLASPLSDKELCTTVRSIYSYSTESAQDTIYHKGGAMLKMPIPVHYDL
                     VEGLMHEQSVNFLSGEEGCGKSLLAMNLAISVASDKPNFLGYNITKHGKVLFLNNELG
                     FPDFLIRFQKMSVGLNPEQLDNFITPTTVPPLNEYYSELDKLLAAEKPCLLVLDCLYW
                     SHDLKENDSSEMKSLMRQLISLRDKHRLCILVVHHTKKGSRYQKMHNDNMRGAGVFGS
                     ATDSVFQFRRSAIDDTKRLFKPTKLRHGADGLRHAHLIALNDETLWFCDEGKTDEDEH
                     IATLTTPKKDIFEIDFSAIMKPGEKVSRKEILEKCKSLGCDERTIDRKLKEAKDRSII
                     ASVKNGQYTLT"
     gene            complement(6171..6566)
                     /locus_tag="PK754_00865"
     CDS             complement(6171..6566)
                     /locus_tag="PK754_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08085.1"
                     /translation="MHNKPNPSTVKSQIHIHPSGHVYLATETRKRFIGKIITREFHCE
                     RDPERHILRVNNSIGFNLELMRMSEQFDWVIITGLSSQPIIVTRDFILRKGFLPSFKN
                     QSYELQIFVKLNDLKKADTEPDLFSPEAA"
     gene            complement(6772..7686)
                     /locus_tag="PK754_00870"
     CDS             complement(6772..7686)
                     /locus_tag="PK754_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08086.1"
                     /translation="MGKKQKKSKPRADSGIQMRSINIEDLNHKYGYDSKSTKPVKGFD
                     SIEYSMYRTRLIGHVDGGIFNEVNGLYSQKRFWAYLTKVLKPNAQRIAFFKDLMKGIP
                     NGTALKSQFDSAFKNFWKSLRQEEIEIELQGDTISLPSYLNLLLFDEFKKTYYHRVFS
                     LIVENTVFFDLKIKPSKEAGKVLSELAKSLANEIKRGLSLELEGFFGYQIRLAKIAVG
                     DPQQIKREFEKLMRYNQKVKVYCEEKHFPKGVLLPIEVRYNLVKHLKKVTYPSSEFSS
                     KGQGAKDMILINKILNELGYTTKRRSHS"
     gene            complement(7881..10850)
                     /locus_tag="PK754_00875"
     CDS             complement(7881..10850)
                     /locus_tag="PK754_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNB08087.1"
                     /translation="MINTEKIFESAIEDHLLESGWLKGRAEDFSRDHALDAGQVIGFL
                     KDSQPKDWEKIAKIHQSDVDTKVVNRLSKELELRGTLDVLRYGFTDSGVRFRMAYFRP
                     ETGLNPDTLALYAKNRLIVTRQVKYSPKNENSIDLLLSINGLPVATAELKNPFTGQTF
                     KNAIRQYQHDRDPRELIFQFKKRALVHFAVDPDEVHLTTKLDGSHSRFLPFNKGHNNG
                     AGNPPNPAGYKTAYLWEEVWQKDSWMDIIYRFMHLQVEEVKVGRKREKKESLIFPRFH
                     QLESVRDLTNDARKNGPGKNYLIQHSAGSGKSNSIAWLAYHLSSLHDATDKRIFDSVI
                     VVSDRTVLDQQLQDTIYQFDHKSGVVQKIDENSNQLAESIKAGTPIVITTLQKFPFIL
                     DKAKALPGRNYAVIVDEAHSSQGGESSKKMKEVLSAQSLEDAEKEEGAETDDGEDEIR
                     KSMAARGRQSNLSFFAFTATPKAKTLEVFGTKGADGKPRPFHLYSMRQAIEEEFILDV
                     LKNYVTYKTFFKLSKAIEDDPELNKKKAAIAIGRFVSLHPYNLAQKTEIMVEHFRQVT
                     SKKIGGKAKAMVVTGSRLHAIRYYFEFQRYIKEKSYSEIKALVAFSGKVIDTGYPEGV
                     SESELNGFGEKELPDKFETDEYQVLLVADKYQTGFDQPLLHTMYVDKKLSGVKAVQTL
                     SRLNRTCAGKEDTFILDFANDVETILESFQPYYELTTMEQTTDPNLLYDLRHRLDEAQ
                     VYLQSEIDALAKVFYNPNGSTKDQARLNALVDPAVDRYEDIDADEKKDEFKKSLTAFV
                     RLYAFLSQVMPFSDIELEKFYVYARLLVSKIQSPDYGGRLKLDNDVALQYYRLQKTSE
                     HLMVLEKLPEYSLDPTTEAGIMREKEMKAKLSEIIEILNTRFGMDLDEADKLFFDQIE
                     VELLQDETLRQQAVTNSIDNFRYGFQEKLDSTMLDRMESNQEIFQKVMENDEIKTAVL
                     EAMIKQVYAKFNNKNLQ"
     gene            complement(10847..11992)
                     /locus_tag="PK754_00880"
     CDS             complement(10847..11992)
                     /locus_tag="PK754_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNB08088.1"
                     /translation="MDIKDFKSGIFRQQYEYKSFLPNSIHREWVVSDARLNNLLSEAN
                     LTLGRLDAFSRLIPDVDFFIRMHIVKEATQSSRIEGTQTTMEEAFLKEADVNPEKKND
                     WAEVNNYIFALKYAIGELKKLPLSTRLLRQTHEVLMQGVRGRHKMPGEFRSSQNWIGG
                     TSLKDAIFIPPIHTEVPELMSDLEKFLNETRLQVPHLIKIGLAHYQFETIHPFLDGNG
                     RLGRLLITLYLVRFGILNGPTLYLSDFFERNRSLYYDNLMQTRLQHNLPHWLTFFLTG
                     VLQTATNSIETFEKILKLQEDIEGKRILKLGKKSEAAKTLVKHLYKNPLVTASDVEKI
                     LHVNISTANRMIDDFVKLKILKERTGFKRNRIFSFHEYLGLFYNPRK"
     gene            complement(12150..13484)
                     /locus_tag="PK754_00885"
     CDS             complement(12150..13484)
                     /locus_tag="PK754_00885"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNB08089.1"
                     /translation="MKAYPKYKPSGVEWIGEIPEKWELTRMKYVAPLQGGYAFKTEAF
                     KTEGIAIVKMNNLKRGRLDLSDAVRVDFKAAVDAFSLKAGDLLFGMSGSLGETGSLGN
                     FAVVNLVDIPCQLNQRVGRFVALQNCSLDFVRFLITSVSFTEPIMSSSTGTAQFNISS
                     EQIGNILISLPPLPEQTAIADFLDRKTAQIDGFIAKKQKLIELLREERVAVINHAVTK
                     GIDPKAKMKPSGIDWLGEIPAHWEVKRLKFFANVNPTKCNYVFDKSSNELVVFLPMEN
                     VLEDGQIIQDLRKPINELSTGYTYFEKNDVIVAKITPCFENGKGALLDRLETPFGFGS
                     TEFHTIRPVKAVSTNEFLYFLTKTDLFMKFGEAFMVGAAGQKRVPPNFVENFMIASPS
                     LDEQNHIVAFLKSETAKIDRTIATIEKEIELIQEYRTALISEAVTGKIDVRG"
     gene            complement(13481..14131)
                     /locus_tag="PK754_00890"
     CDS             complement(13481..14131)
                     /locus_tag="PK754_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HNB08090.1"
                     /translation="MDKAWFQKVFSEERTQKYFETYPANDKKAIEHYACNIMISEAFY
                     PALSIFEVALRNSVNRELETMFHTQDWYQQIPTTAGLVKLNKDISNAKRQIMMRNEAI
                     TASKVVAELTLGFWVRLFNTEYERILWKNLRLAFPYLEKRKRQRHTVSAPLNRIRDFR
                     NRVFHNEPVCWNINSLENNHKTIVETLFWLNKDLPDYLNTTERFHTVLNEVRKILS"
     gene            complement(14243..15955)
                     /locus_tag="PK754_00895"
     CDS             complement(14243..15955)
                     /locus_tag="PK754_00895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013554240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNB08091.1"
                     /translation="MTIQSHREKANFIWQIADLLRGNYKQSEYGKIILPLTVLRRLDQ
                     ILEPTKAKVLQVYDDNKKQKPEILEKLLNKAAKQKFHNRSKFDFKKLAADPNGLEKNL
                     RNYINGFSAGAKEIFDYFQFEDEIEKLDKENLLYLVIKDFAKQDLSDLDTMQMGYVFE
                     ELIRKFSELSNETAGEHFTPREVIRLMVDLLFIGDNDILRKEGIIRTLYDPACGTGGM
                     LSVAEAYVQELNPNARLEVFGQEINDESYAICKGDMLIKGQNPGNIKYGDTFTKDGLE
                     NELFDYMLCNPPFGVEWKKQKEVIEKEHDDRGFAGRFGPGLPRINDGSLLFLLHMISK
                     MKKDGSRIGIVFNGSPLFTGSAGSGESEIRKWIIENDMLEAIVAMPDQLFYNTGIATY
                     VWILTNRKAKKRKGKVQLINAVSFFEKMTRSLGNKRNLISETQLTSIAKIYGSFKSGE
                     FCKIFDNEDFGYARITVERPAKDEKGQVLTDKNGNPKADSELRDYENVPLTEKIETYF
                     KREVLPHVPDAWVDESKTKIGYEINFTKYFYQYKPLRSLSEIRADILALKKETEGMIR
                     EVIK"
     gene            complement(16172..17197)
                     /locus_tag="PK754_00900"
     CDS             complement(16172..17197)
                     /locus_tag="PK754_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNB08092.1"
                     /translation="MFLSKHPNGTYYLWYVDENNKKQKISTRSKTKADALKFLKDFNL
                     EKYRQSERIRCIHLSEFTCDYANYSKANHRPTTTRTAITALKELSRKMGNPLLSRISV
                     HDIESFFTSKAQECNSWALLKYYVTLKSIFNTAKRWEYITVNPFSKIPKPRPAEQTPV
                     YFTHADFTKLLRAIDDSDFKELVLFALMSGMRQSEIVHLTWSQIHFEKRLIQIRNTEC
                     FTTKSGKNRNVPIGEELLILLEMKYQNTMEKHSYIFQRNGKPYRHEYVSKKLKQYIRK
                     TELDDRLHFHSLRHSFITWALERGASLHQVSKVAGHSSTAITEKVYGHIKAEELHHVA
                     NLITINK"
     gene            complement(17331..17403)
                     /locus_tag="PK754_00905"
     tRNA            complement(17331..17403)
                     /locus_tag="PK754_00905"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17368..17370),aa:Phe,seq:gaa)
     gene            complement(17639..18226)
                     /locus_tag="PK754_00910"
     CDS             complement(17639..18226)
                     /locus_tag="PK754_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08093.1"
                     /translation="MKNLLKIFTFVLATNAMVTAQTIEVANEEPLTPEPKSTYYETKN
                     RIGFAAGNVSGVGLSYERDLFRNFSALFVIGGVAQKSQSDFNTGLTFKYTFSRINKRF
                     SVNVVSGGSYFLDIDDLGVYDNTNENRSLERRRSYIKMGLGLGMTALFFDGRLGIDVN
                     FIGMGASFQKKQNKKDYQLGDKPIRGPQVNLTYHF"
     gene            complement(18426..19820)
                     /locus_tag="PK754_00915"
     CDS             complement(18426..19820)
                     /locus_tag="PK754_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNB08094.1"
                     /translation="MKKYPVLSLRERVRPHTDYDGSGVTIAFVDSGYYPHPELRGRIL
                     KFVDVSSEAFTEKDFHTIRPSSWHGTMVACSACGNGIWSKGYYRGIASGANVVLIKAF
                     DGKKVKSSYILKSLTWIIAHHKPYHIRVVSVSVGGDRNEPSEKSRICKAIHRLHQLGI
                     TVIAAAGNNPSRPVIPPASCRFAIAVGGIYDHNDKNPDTASEYGSSYGITLDGLSKPE
                     IVAHARFLPAPMLPDNFTYEESQILHVLYKTPANNLKRKLRSLIHKTRLDAAILSLNP
                     AEIKKAIRQRMDGEKFIAPGFQHVDGTSFAAPIIAAVVAQMLEANPLLTPTMIRMILE
                     TTAKRVPGIAPEKQGFGLVQAKACVEQALIETSMPEIHESPLILKDRIVFLYYHRNIR
                     KVNLVGDFTDWQKNKLLLVEIRPGVWRVDIPLLSAGQYHYKFFVDDTLWTEDPLNPHK
                     ISDPFGGMNNILHI"
     gene            20023..20832
                     /locus_tag="PK754_00920"
     CDS             20023..20832
                     /locus_tag="PK754_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08095.1"
                     /translation="MKSMRLLVLGVLTLTMLGCDSNNSNTLIAYVYQHEYLGSEGNDF
                     DYDGIVMGDPLPFIESVTAKNITGVQELYVSQNSFDFDFLYDATFGDGDITIKTTEGT
                     ITGTVDQPTMYDSVKASTTDYYIPLNTALTLTTYGGNSDFKYISAELEYYDGESWYYV
                     EMDSAFEGNTFTFPASMLDTESYLYIYGIDCANGPIAKAGSSGNMNGDGSGFMWHIIS
                     DDNFYMSFAVGIEAGSNVKKSSSHVQERRTREDRKRRFQEKTAKILNASAM"
     gene            complement(20888..21397)
                     /locus_tag="PK754_00925"
     CDS             complement(20888..21397)
                     /locus_tag="PK754_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007724476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB08096.1"
                     /translation="MKPMIIAETDRLILRRLTHEDKHFILRLVNEPSWLRFIGDRNVH
                     SPEDAVRYITDGPMAMYAKYGFGLFLVALKGSDTPIGMCGLLKRDTLEHADIGFAFLP
                     EYWGQGYAYEAAAATMAYGQKQHGLNKIIAITSPDNASSIKLLKKLGLVEKEIIQLPG
                     YSGETLVME"
     gene            complement(21440..22282)
                     /locus_tag="PK754_00930"
     CDS             complement(21440..22282)
                     /locus_tag="PK754_00930"
                     /inference="COORDINATES: protein motif:HMM:NF023144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HNB08097.1"
                     /translation="MKPVTEINTVHLFPELDRLLIACLRNLRPEDWRVQTSAGSWTVR
                     DVALHLLDGNIRLVSAMRDHHFAPAPNDLSSYEATVQFLNTLNADWLKAFERVSPAMI
                     CELLEITGAWYCAYLKTIRHNDDAVFPVAWAGESVSKNWFHVAREYTEKWHHQQQIRS
                     SLGDEETLLQNAWYLPYLDTSMRALPHVYRNMPAPDGIVLQFSVSDLTDANWFLKRNN
                     NKWELFSGTDSKPHAHMRIKKKAAWRIFTKNLNGERARPYIHMLDGDEFWLNPLLNMV
                     AVMA"
     gene            complement(22343..23722)
                     /locus_tag="PK754_00935"
     CDS             complement(22343..23722)
                     /locus_tag="PK754_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNB08098.1"
                     /translation="MKQWFCVFLIIMVASCQKPALKQPDVKHLEPAAMTLMKQGLNDL
                     HAYTMLSELTTKAPKRLSGSTMADSAVAWGERTMKNLGFENVRLEPVMVPHWVRGPVE
                     EAFITSTAPSQKLNICALGGSIATPKEGIESEIIEVKSLQEVRTLGDKVKGKIVFYNR
                     PFDKTLFAPFEAYGGAVDQRGYGAVEAARFGAAAVLVRSMTNRIDTYPHTGAMRYNDS
                     IPKIPAAAISTQDANNLSAMLTKGQSVKVRMKLTCETLADKLSYNVVGEIRGTEKPEE
                     VIVIGGHLDSWDKGTGAHDDGAGVVHSIEALRLMKATGLLPKRTVRVVLFMNEENGVR
                     GGKGYAAVERPNEKHIAALESDGGGHAPRGFGIEADSVKLPRITRFESLLKIIDADRV
                     GPGHGGTDIEPLGKTGVPCIGLHPDPQRYFDYHHSDHDTIDKVHDRELELGAIAMAIL
                     TYAIAFEGI"
     gene            complement(23757..24410)
                     /locus_tag="PK754_00940"
     CDS             complement(23757..24410)
                     /locus_tag="PK754_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08099.1"
                     /translation="MFIGHFATGFISKKWMPHISLPVLFIAVQWPDLIWPVLLLLGWE
                     SVRIEPGNTVMTPLAFDHYPYSHSLMTNLLVASLFALWALRRYRDQRVAALLFITACS
                     HWILDFVTHRPDLPLSPGSSQMFGLGWWNIPPVAVVAELALMAAGIFIYHRNTRATGK
                     TGVWAFWGLIVFLMIIHTGNLLGPPPPNITGIALAGNLMWLFVLWAYWIEKNRTVIK"
     gene            complement(24450..24890)
                     /locus_tag="PK754_00945"
     CDS             complement(24450..24890)
                     /locus_tag="PK754_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08100.1"
                     /translation="MKICPNCTTNNLDTSAYCVSCGAELTIPVKEEPARESRFPKDHI
                     THREDHPASGMAKLAMTLGLISLFFVFFAVICAPLPGIMAIIIGNNELKKLRGERYST
                     NGETYCQIGIWSGWISTAIGILLIAIGGIATIWLVNWFIEAFTR"
     gene            complement(25016..26188)
                     /locus_tag="PK754_00950"
     CDS             complement(25016..26188)
                     /locus_tag="PK754_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNB08101.1"
                     /translation="MAGKFKNVDYYDMDTLLSDEERMTRDTVRDFVDDKILPIIEEHN
                     RNGTFPMNLVKEMADLGVFGANLPEKYGCAGMNNVAYGLIMQELERGDSGVRSFASVQ
                     GALVMYPIYAYGSEEQKNYWLPKLAKGEKIGCFGLTEPDNGSDPGGMKTKAVKDGKHY
                     ILNGAKMWITNGTVSDVAVVWAKLDGVVRGFLVEKGTPGFTAPEMKGKFSLRASVTSE
                     LVLQDVRIPEENILPNGSGLKAPLGCLTQARYGIAWGALGAAMACFDSSVRYAKERIQ
                     FDKPIGSFQLVQEKLAYMITEITKGQLLVLQLGRLKDSNKMKFHQVSMAKRNNVYIAL
                     EIARIARDLHGANGIIDEYPIIRHMMNLESVKTYEGTHDIHTLIIGESVTGIPAYK"
     gene            complement(26217..26678)
                     /locus_tag="PK754_00955"
     CDS             complement(26217..26678)
                     /locus_tag="PK754_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08102.1"
                     /translation="MKKWMMIAALITAFTFSQNANAQLPVKMGPRAGVNIGDGGDYFV
                     GGHIEVSLPVLPLTFVPNAEYVFVDNATSINTGADVQYTFIGVGVAKLVAGGGYTLNY
                     NKPKGSDSATDSGFNLQFGAKASLGVSAFGLIRATNIGGNWTKAMVLGVTF"
     gene            complement(26784..27197)
                     /locus_tag="PK754_00960"
     CDS             complement(26784..27197)
                     /locus_tag="PK754_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08103.1"
                     /translation="MKNSRILVLFCLSLMATAIHAQSDPFTGSYTGKGFRPGEKYTEK
                     PSYVIDVTIEKTGDYYMVRWFENGNLSYYGLGIHIDNILSVSYVSVDKSIYGTVSYKD
                     FKKDKGYLVGAWCIAPMDPEAVGNGKNGMEVLWPK"
     gene            complement(27242..28984)
                     /locus_tag="PK754_00965"
     CDS             complement(27242..28984)
                     /locus_tag="PK754_00965"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HNB08104.1"
                     /translation="MTKKSVGNFILVLHTHLPYVLHHGQWPHGMEWLYEAASETYIPL
                     LNALNALQNEGRRPRLTISMTPVITEQLASETFKKEFVAFLYDEIDRARTDMVSFEKS
                     PTEKKLVPLAEYWLDFYQNTLKDFTLTYRHDLIGAFRTLQDQGVLDIITCGATHGYFP
                     LLGTDANIHAQIRMAVVTHEKYFGRKPRGIWLPECAYRPRYVWTPPVASALGSEPRLR
                     KGIEEILDANSLEYFIVDSSLVEPGRAVPMYMGRFENLRKLMMRLASENRPIPKADFL
                     QSVYDVYKTKSSYDAPGNPVSIFARDMETSMQVWSSEQGYPGGEWYLDFHKKHEQSGH
                     RYWRVTSLEADLGLKAPYEPQKIQSAIADDAQHFVQAVRQALTRFKTKHGYEGTLTAP
                     FDSELFGHWWFEGPGFLKTIFELMDDDPAIQTMHCAESLDQNPPEKIIAIPEGSWGAG
                     GNHYVWFNHEVDWMWPFIYNDEAAMRDHVQRWNNNTDPEWTAIIKQMARELFLLESSD
                     WPFLISTQSAIDYATERFMEHHTAFEKLARLSEYHCNGSALHPDDRAWLREITSQDHI
                     FDDALIDPNWFLND"
     gene            complement(29136..30389)
                     /locus_tag="PK754_00970"
     CDS             complement(29136..30389)
                     /locus_tag="PK754_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HNB08105.1"
                     /translation="MSTWLVEKCKPFSNFVEDIKAKGLYSYFTELERTGTYITVKGQN
                     LVNFSSNDYLGLTHDPRVIEEARKALDIYGSGLCGSRYVVGTTIEHTRLEEKIADWKQ
                     KENCIVFSTGYQTLLGTVSAMLVKADDSIEDDIVLAIDKLSHACILDGCALAVGTHMT
                     RGGSTKNLDGQIRYFRHNDPGSLESIVESSVKRGKNVMIIVEGVYSMDGDICRLPDFV
                     NIANKYDACIAVDDAHGTGVLGKTGRGTGEHFGMDKDIDLLMGTFSKALGGVGGFVCG
                     QRDVISHIKHKARSLIFSAASPVSNIVSVSKIIDIIRSEPEHLQNLRVNESYFKSKLK
                     EFGFNYGDSETPIVPIIIGDSEKTMYISKKLYENNILMLPMIFPAVARGQERLRCTIS
                     AKHTRQDLDHALDVLYTEGKKIGVI"
     gene            30805..31179
                     /locus_tag="PK754_00975"
     CDS             30805..31179
                     /locus_tag="PK754_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB08106.1"
                     /translation="MSGTLKRILIVDDEETLTFSLYQSFIISKENYEVVTASSGTEAW
                     EKFAEHPFDLILTDITMPGISGIELLKRVKKERPPTHVIIMTAYGSDEKKEEALSFGA
                     YRYIEKPFEIKMMKSVIAEALK"
     gene            31197..31859
                     /locus_tag="PK754_00980"
     CDS             31197..31859
                     /locus_tag="PK754_00980"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNB08107.1"
                     /translation="MALVGNLRDLKLTNIVQLNCMEKNEAMFTVELRNAIGRIYFAEG
                     NIVHATFGTVEGEDAVYKILAIKEGPFKVENGVASPKRTINAPWGNLMMEGLRLIDEA
                     LEAKGEAMAKFTRDIKTINGVNAVLVSSPQGEILMEDGIANGKRTSAGMGFIMRKLAK
                     IGRASKSGTYKFGIVTGKNDRKIISKWHDDIITLTVEQKAAADLIEQAIEKAPGIQKV
                     SE"
     gene            31881..32318
                     /locus_tag="PK754_00985"
     CDS             31881..32318
                     /locus_tag="PK754_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08108.1"
                     /translation="MKDKLEEINSVNGVKASFIYFNDGETVYANMPQGFDEATYVEIA
                     RDVIQVAAMFDRLSTPLHEYDLKYDNGRVIAYPMSNFNLIILCQPNVSVAMLRLTVNV
                     ALADLEADKKFQKRLSKVEASRRSFLVRSNMDAESWALVEVVN"
     gene            32341..>33106
                     /locus_tag="PK754_00990"
     CDS             32341..>33106
                     /locus_tag="PK754_00990"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNB08109.1"
                     /translation="MATVLSGNLSHVDVLSIVKLLVAGDRTGRILLERGDQRENGEIY
                     LANKRIVHAVCDTYLGEPAFHELILWTSGKFSFEPDVASLQKSIDKETGKLITEATLE
                     YEAWQKIARHIPSFRVKFRKTDNEPSPTIRLKAKDWELLHLLEKDEYSVDELSSRLGM
                     KDMDVAGIVYMLAEAGLITAVSGATASADKQNVSSAFFKNLENELIQLIGPVASIIID
                     DVVETFGEDQKTFPKDKVPPLVEAIANEIYDPAKQLA"
BASE COUNT         8858 a         7311 c         7514 g         9423 t
ORIGIN      
        1 tttgtggcag cccctgatcg gtcgtccaca tcgtatgcat gtattgtgtc acgcgccgat
       61 ccggatccag cgcgtataag gaaccggtga cgaaaaggca atgtaaccaa atcagggtta
      121 tttgaacacg gcttctaaga ggatgacatg attgcataaa tacactatga tttttttata
      181 tgtaattcaa aatggatatg ccgcctttgc caacttgaat tcaaattcaa actcggccgc
      241 tacagtgagt ggcgatccgc cgttttattg aaaattcaat ccggtcggat cggagttggt
      301 tgtatgatgg gtctttcctt gtataaacga taaaatgatt cgtttgctat gaagtgaatc
      361 ttggtaactc tacgacaaag gtactgcctt taccggattc actttcggcc caaacctggc
      421 cttcatgcat ttcgacgatt tgtttgacga tagacaaacc caagcctgta gatgtctctc
      481 caccggtagg tttggctgaa agacgttgaa atttaccgaa aagttttttc atatcgtcgg
      541 tagtcattcc gggaccttca tccgtgattc gaattatgat tttaagttca ttcatgccca
      601 aatctacgcg tattgtttta tcgtaatctg aatatttgat cgcgttactg atcaggttat
      661 ccagcgcttc gtagagacgg tcacggtcgg cgttaacata gagtccgggt acagtttgta
      721 cctgcacatg ttgacgtttt cgttccagca tttgttgatt actaaaaaga acctgttccg
      781 ccaaatcgga tacatctaat ttttcttttt gcaggacgac ttttccgctt tccaggctaa
      841 ccgtattgag aagaccgtta ataagatgaa gcatgcgatg ggccgccttg cgaatataac
      901 ccagatcgcg catggactga gctttatcgt catacgcttc gatgatcaga tcggtataac
      961 ttatgatagc cgctaacgga ttttttaagt catgggatgc cataccgaga aattgtgttt
     1021 taagctcatt ggcgcgcaac gcttcttcgc gttgacgttc ggcttcgatg cgcgcgtcct
     1081 cggtgcgttt tttttcttct tccagattat gcgtgcgcag tttgaccaat gcggctagtt
     1141 ccttttcacg gtgcatcaag tggcgaacac gccaacggta taatgataaa gccgcagcga
     1201 tcataccgcc gaaaatgaga agcaaaaacc accgtgtttc gtaaaaatgc ggacgaatcg
     1261 taaaagaaaa cgaagtcggc tccgatgccc agatgccgtc gttgttgcag gcgatgactt
     1321 taaaagtgta ttcgccggga tgaagtcctg tgtagaatgc cgtgcgtcgt gtaccggcat
     1381 ccatccaatc ggtgtcaaat ccttcgagtt taaatttgtg aaatacgcgc tgcggagcga
     1441 gaaaactcaa agcggcgtat tgaaactcaa ttcgcaaaat accgggcggc aataccacat
     1501 cgttttgtat cggccatgat tgcccatcgg ctatgcatcg ttcgatcacc acgggtggcg
     1561 gtacagggtt ggtttttatt ttatccggat cgatgaccac ggcgccttta ggggtgggaa
     1621 accacagctt tccgtcggat gttttgtgcg cacgtcccga aggggttgtt tggctggtct
     1681 tcataccgtc cggtctgcca aaaagaagcg gggtcaattg attggtttta ccgcgcgcga
     1741 catcttcaaa atccttttta ctgatgcgaa aaattccttc tgcaccgcca agccagatgt
     1801 aaccgtgatt atcttcgagg atttcaaatg ccgcatcggc cggcaaacct tggcgtacgg
     1861 tgagcgaagc aaacggcaaa tctgtggcgc gtggatcgta gcgcgtaatg ccgctttgcg
     1921 tcgcaaacca atatacgctg tcccggtcac agtgtattgc aaaaacaata tcgctgctca
     1981 atccgtcttt catcgtcagt actctgaaaa aacctttgtt acgatcgtag cgtgcagcgc
     2041 cgccaccatc cgtaccggcc cacacgttgc catagatgtc ttcgtacaaa cataaaatag
     2101 atgtggtaag caggccgtct tgcatggtaa aagtacggtg gctgccgtct ggtttgatca
     2161 atccgatact gttgatagtg ccgatccaca gatggccttc gcggtcttta agcatatcac
     2221 gaatgttgcg aaaggcgtgg ttaatcgcag acggagccgg cgcgaatcgt ccgctggccg
     2281 gattaaaaat gtctaaaccc gcaccgtacg taccgaccca caggcggccc ctggaatcac
     2341 gaacaatgcc gcgtacctga ttattggaaa gcccgttggc cgtcgtgtaa tttacaaaac
     2401 ggttgcccga atagtgcgac aagccgccct ccgtaccaaa ccacatatcg ccgttatgtt
     2461 cctgtacgat gggataaaca accgggtgca ccattccgtt ggcggatgca taagtgataa
     2521 attttccgtc acgcaggcga ttgagaccac cgcgaaatgt gccgatccat aaactgcctt
     2581 cccgatcctc aaaaagcgcc gaaattcgat tattggttaa tccttctttt tctgaaaact
     2641 gctcaaggga aatcaattcg ttttcgtcgc ggaaacttcc gggtttggcg cgaaaaagac
     2701 ccataccatc cgaacccaac caaacattgc cgtcacggtc ataccacaca cgccagatgg
     2761 attccggggg gagcccgttt ttagttgtaa aatggcgcag ttgattttct gaaatttgaa
     2821 aaacgccggc gttggtggtg ccgatccata tcctattatc gcgatctaca tacatcgaac
     2881 ggatgcgctc gccgatgttt gcaaactcct caaggcgacc ttcgacataa cgataaatag
     2941 tatttgtagc gcctatccat acggtaccgt cgtgatcggt gcacaaccca aaaacatcct
     3001 gcagtacatt gctgcgatac atcacgggct caacggcaaa actatcttgt gaaataattc
     3061 gtattctgtt taatcccttt cgggtgcccg cccaaaccat ttcatcctga tccatcgcca
     3121 gtgataaaac attgtcattg gtgagcccgt tggttacggc aaggcgttgg aagttaggat
     3181 ctttaaagcg ataaataccg ttgccgttgg ttcccatcca aatataaccg tgtttatctt
     3241 ctaagattcg ccagagcccg taactgagaa aagcatcaat ggaacggcga ttgaaaagcg
     3301 taaattttgc gccgtcaaaa cgcgccaacc cttcaaacga accgatccat aaataccctt
     3361 cacgggtttg ataaaacgac agcacggaga ttccgggtaa accgtcttct ttcaaccaaa
     3421 catcgtgcac gtattgcgtt acgctgcgct ccggatccag cgccttcgta tcggcagcca
     3481 acatgaaaag aaaaaggaaa acacaaaaca gaaggcggaa tattttttca gaagtgatac
     3541 tcatgaagct ttactttcgt aatagcgtcg gcagtttcaa aaaaggtagg ggtaaccgga
     3601 aagcacgaac attgaaaccg atgtgaggca tgggacgtgc ccggtcacaa ttgcatataa
     3661 cttacaaaaa aatgacccat aagttcaaga aatgaaaaaa tatcaccacg caatcggtta
     3721 tagccatacg ctagttcaca aaaaataaga ccatgtgaaa taatgtcata ggacataaac
     3781 taaaaaccct taagctgttg taacttaagg gtctttgctg tggctccgga cggaattgaa
     3841 ccgccgacac aaggattttc agtcctctgc tctaccaact gagctacaga gcaaacaatg
     3901 agttacaata cttaactaaa ccagtggtta cctttttggt tacctatatt tccgaattgt
     3961 caaataacaa acgaaaaaac cgctaagcgt gcgcaaggac taccacatcc tattacgcct
     4021 aacggtttct cgatttacaa ttttattttg tgtggtagtt atgcacgctt gctttaacgc
     4081 agtataaaag aaaaaagttc cagtatggta tttgtgtgtt attgacgagt attagtaata
     4141 gtgttagaat ttaaaattga tataccccct atgccaaaaa aaaagagcgg gtggttattg
     4201 aatttaaaat ctgcatgttt atatgcataa gtcaatattt gacctccctg gaagtttgac
     4261 acggcgtcat aggaaaatct atgtctatgt caaactctta aatagttcta tctgactttg
     4321 acattgacag tgtttttatt acgatattca tgtcaaagta tattgaccat tcttaacgga
     4381 tgcgattatg ctacggtctt ttgcttcctt gagttttctg tcaattgttc gttcatcgca
     4441 tccaagcgac ttacattttt ctaaaatctc ctttctggaa actttttcac ccggcttcat
     4501 gatggctgag aagtcaatct caaatatgtc cttttttggg gttgttaatg ttgcgatgtg
     4561 ttcatcttca tcggttttac cttcatcgca aaaccaaagc gtttcatcgt ttaatgcgat
     4621 taggtgtgca tgtcgaaggc catcggcccc gtgtcgtagt ttggtcggct tgaataaacg
     4681 ttttgtgtcg tctatggccg aacggcgaaa ttgaaatacg ctgtctgtag cagagccgaa
     4741 tacgcccgcg ccgcgcatat tgtcattgtg catcttttga taccgagaac ctttttttgt
     4801 atgatgtact accaaaatgc aaagccggtg cttgtctctt aaagatatta actgccgcat
     4861 tagtgacttc atttcactcg aatcgttttc tttcaagtca tgcgaccaat aaagacagtc
     4921 cagtacaagt aaacacggtt tttcagcggc aagcagtttg tcaagctctg aataatattc
     4981 attgagaggg ggaactgtag tcggtgtaat aaaattatcc aactgctctg gattcagccc
     5041 gacggacatt ttttggaaac gaatcagaaa atctggaaac ccaagttcat tatttaagaa
     5101 aagcactttt ccgtgctttg tgatgttata acccaaaaaa ttgggtttat ctgacgccac
     5161 agaaatggcc agattcattg ctaaaagcga tttcccgcat ccttcttcac cggataagaa
     5221 attgactgac tgctcgtgca ttagaccttc tacaaggtcg taatgaactg ggataggcat
     5281 tttaagcatc gctccgcctt tgtgatatat agtatcttgt gcgctttcgg ttgaatagct
     5341 gtaaatagag cgcaccgtcg tgcatagttc tttatcggat aggggactgg ctaagttttt
     5401 gttccaaagc tgaagaatag ctttcgtctc gctaatagga aggccagcat gcttcaaatg
     5461 acccgcaata cgtgttacgg tgctatttct ttcgcccacc ggaacgccat tgcttaatat
     5521 ctccgaataa tctgtttttc tcgacacttt tgccgaagta gtttccttta actcaagatg
     5581 atagaaatcg gccaattcat ctaaagtttt gcgggagttg ggtttaaatt cagcatcgta
     5641 tttttttcct gatgcgtcaa taaaaaccgt cttgttctcc gaaacatctt ttccgaaaaa
     5701 gggcagccaa atgaagtttc ctttatctcc ggcattttgt tttggaaata tttcaagagc
     5761 ggtttcaggt tttagaacct gaataatgaa tgctgatagt actgaccgaa tgaattttgc
     5821 tgaaatatct tcatcaaaaa aaatccatag atgataattt ccgtagccgc ttttggatat
     5881 ttcacgatac acatagagac ctgcttttct gagatattct tcgcattgac tactgagttt
     5941 gtccatagtc tgccgatatg catccggctt ttgatgggaa tcaaaatcaa tgaccgcaaa
     6001 gcgaattaca ttattgggta gtaagttata aaaacctaag cggtcaacgc cgtcaagatg
     6061 tctttgaaca tagtcaacca agggccggtt tacttgtaca gacattccgg aggacgtgcc
     6121 gacaaaatca acacgccctt taaaaatgtc taacatgtct tctgtggtca ttacgcggcc
     6181 tccggagaaa ataaatccgg ctccgtatca gctttcttca ggtcattgag ttttacaaag
     6241 atttgtaact cgtagctttg atttttaaat gaaggaagaa atcctttacg aagaatgaag
     6301 tcacgcgtaa caataattgg ctgagaactt agaccggtaa tgataaccca atcgaactgc
     6361 tccgacatcc gcatcaactc aagattaaat ccaatcgaat tgtttactcg aagaatgtga
     6421 cgctccgggt ctcgctcaca gtgaaattca cgagtaatga tttttccgat aaatcgcttt
     6481 ctggtttcag tagcaagata gacgtgccca cttggatgga tgtggatttg acttttgacc
     6541 gtggaagggt taggtttgtt atgcataaag tactttccga acgggtcaga gttgacgctc
     6601 tggcccgttc tcttttaaaa gcaaaacagc cgacggagga aatgggcata acctcgcagc
     6661 ggctgttttt acactgagta aaaatttatt ttgcccattt ttcatggaca aaatttatta
     6721 cccgggaaga gatatactta gaagtataat tatacttcta aagtataatc atcaagaatg
     6781 tgaccttcgt tttgtagtgt atcctaattc attgagaatt ttattaatta gaatcatatc
     6841 tttcgcgcct tggccttttg aactgaactc gctggacgga taagtgacct tcttgaggtg
     6901 cttcacgaga ttgtatcgca cttcaatcgg aagcaggacg cctttgggaa agtgtttttc
     6961 ttcgcagtaa accttaactt tttgattgta tcgcatcaat ttttcgaatt cacgtttgat
     7021 ttgttgaggg tcgccaacgg caatttttgc caatcgaatt tgataaccaa aaaaaccttc
     7081 gagttcaagt gacagccctc tcttgatttc atttgctaga gattttgcca attcagataa
     7141 aactttaccg gcttccttag agggctttat ttttaagtca aaaaaaacag tgttttcaac
     7201 aattaaagaa aatactctgt gataataagt tttcttgaat tcatcgaaaa ggagcaaatt
     7261 caaataagat ggtaaagaaa tggtatcgcc ttgaagttct atctcgattt cttcttgacg
     7321 gagggacttc cagaagtttt tgaaagctga atcgaattgt gattttaggg ctgttccatt
     7381 cgggattcct ttcattaaat ccttaaaaaa agcaatcctt tgggcgttgg gttttaaaac
     7441 ctttgttaaa taagcccaaa agcgtttttg gctgtataaa ccgttaacct cattgaaaat
     7501 accgccgtct acgtggccaa ttaatcgagt gcgatacatt gaatactcaa tagaatcaaa
     7561 acctttaaca ggctttgttg atttcgaatc ataaccgtat ttatggttaa ggtcttcgat
     7621 attaatcgac ctcatttgaa tgcctgaatc agccctcggt tttgatttct tttgtttttt
     7681 gcccattcag atttaaaaca aaaattctgc acccatccgg tacggacccg ctacaggtca
     7741 tcagacaatg cggaagtata aaatttatat tgtagcgtat tggtacttaa ctgaatataa
     7801 atcagttgaa gtcctttgca aagcgaaatg cacttaggag cgcattttgg agtattaata
     7861 gttattgagg ggggggtgga ttactgaagg tttttgttgt taaacttcgc gtacacctgt
     7921 tttatcatgg cttccagtac ggcggttttg atttcgtcgt tttccatgac cttttggaag
     7981 atttcctgat tgctttccat tcggtcgagc atcgtactgt cgagcttttc ttggaaaccg
     8041 taacggaagt tgtcgatgga attggtgacg gcttgttggc ggagggtctc gtcttgaagt
     8101 agctctactt cgatttggtc gaagaataat ttgtctgctt catccaaatc catgccaaag
     8161 cgggtattca aaatttcaat gatttccgaa agcttggctt tcatttcttt ttcgcgcatg
     8221 atgccagcct cagtcgtagg gtcgagcgag tattcaggta gtttttccag caccatcaag
     8281 tgctcagaag ttttttgaag acggtaatac tgcaaagcaa cgtcgttatc cagtttcagc
     8341 ctaccgccat aatctgggga ctgaattttc gatactaaca atcgcgcgta cacgtaaaat
     8401 ttttccaatt cgatgtcgct gaatggcatg acctgtgaca ggaacgcata gagccggaca
     8461 aatgccgtaa gcgatttctt gaattcatcc ttcttttcgt cggcatcaat atcttcatat
     8521 cggtcaaccg cagggtcaac caaggcattg agacgggctt ggtctttggt ggagccgttg
     8581 ggattgtaaa acactttcgc aagcgcatca atttcagatt gcaagtaaac ttgtgcttcg
     8641 tcgagccgat ggcggaggtc ataaagtagg ttagggtcgg tcgtttgctc cattgtggtc
     8701 aattcgtagt aaggctggaa cgattcgaga atcgtctcaa cgtcgttggc gaaatccaaa
     8761 atgaatgtat cctctttgcc cgcgcatgtc ctatttaacc ttgataatgt ttgtaccgcc
     8821 ttcacaccgg agagcttttt atcaacgtac atcgtatgta atagcggctg gtcaaatcct
     8881 gtttggtact tgtcggcaac gagcaatact tgatactcgt ccgtttcaaa tttatcgggt
     8941 agttcttttt cgccaaaacc gttgagttca gattccgaaa cgccttctgg ataacccgtg
     9001 tcgataacct taccggagaa tgcgaccaac gctttgattt cgctatagct tttttccttg
     9061 atatatcgtt gaaattcgaa ataatagcgt atggcgtgta ggcgggaacc tgtgactacc
     9121 atcgctttgg cttttccgcc gattttttta gacgtgactt gccggaaatg ctcgaccatg
     9181 atttctgttt tctgagcaag attataggga tgcaaagaaa caaaacggcc tatcgcaatc
     9241 gcggcttttt tcttattcag ttccgggtcg tcctcgatgg ctttagagag tttgaaaaaa
     9301 gttttgtagg tgacgtagtt tttcagaaca tcgagaataa attcttcttc gatggcttgc
     9361 cgcatgctgt agagatgaaa aggcctcggt ttgccgtcgg ctcccttagt tccgaaaact
     9421 tcaagcgtct ttgccttggg tgtagctgtg aatgcaaaga aacttagatt agattgtctg
     9481 ccccgtgcgg ccatagattt tcgaatttca tcttctccat cgtctgtttc tgccccttct
     9541 tccttctctg cgtcttcaag ggattgagcc gacaaaactt ccttcatctt tttggaggat
     9601 tcgccgcctt gtgagctgtg ggcttcatcc acgatgaccg catagttacg gcccggcaaa
     9661 gcttttgctt tgtcgagaat gaagggaaat ttttgcaacg ttgtaatgac aatcggagtt
     9721 cctgctttga tggattccgc gagttggtta gaattctcgt cgattttctg aacaacgccg
     9781 gatttatggt caaattggta aatcgtatcc tgtaactgtt ggtcaagcac cgtgcggtca
     9841 cttactacga tgacagaatc aaagattcgt ttatcggtgg catcatgaag gctggacaag
     9901 tgataggcga gccatgcaat cgagttgctt tttccgcttc ctgcggaatg ctgaatcagg
     9961 taattttttc ccggcccgtt cttacgcgcg tcgttggtca agtcgcggac ggactcaagc
    10021 tggtgaaagc gggggaatat cagggattcc tttttctcgc gttttctacc gaccttgact
    10081 tcttcgactt gcaaatgcat gaatcggtaa ataatgtcca tccaactgtc tttttgccag
    10141 acttcttccc atagataggc tgttttataa cctgccggat tgggcggatt tcccgccccg
    10201 ttattatggc ccttgttgaa tggtagaaat cgtgaatgcg agccgtcgag cttggtggtc
    10261 aggtgaactt catccgggtc aaccgcaaaa tgaaccaaag cccgtttttt gaattggaaa
    10321 atcaattcac gcgggtcgcg gtcatgttga tattggcgga tagcattttt gaaggtctgc
    10381 cccgtgaaag gatttttgag ttcggcggtc gcaaccggaa ggccgttgat gctaagcaat
    10441 aaatcaatcg aattttcgtt cttggggctg tacttgacct gacgggtaac gatgaggcgg
    10501 ttctttgcat acagcgcaag cgtatcggga ttgagacctg tttccggcct gaaataggcc
    10561 atgcggaacc tgacgccgct atccgtaaat ccgtagcgca aaacatccag cgttccccgg
    10621 agttccagtt ccttggataa acgattaacg accttagtgt caacatcaga ctggtgaatt
    10681 ttagctattt tttcccagtc ttttggctgg ctgtctttga gaaaaccgat aacctgcccg
    10741 gcatcgagcg cgtggtcgcg actaaagtct tccgcacggc ctttgagcca gccagattcc
    10801 agaaggtggt cttcgatggc tgattcgaag attttttcgg tgttaatcat ttacgaggat
    10861 tataaaatag tccaaggtat tcatgaaaag aaaaaatccg gttgcgtttg aacccggttc
    10921 gttccttgag gattttcaac tttacaaagt catcaatcat acggttcgcc gttgaaatat
    10981 ttacgtgtaa tattttttca acgtcagagg cggtaacaag aggatttttg taaaggtgtt
    11041 tgaccaatgt ttttgccgct tcactctttt ttccgagttt aagtattctt tttccctcta
    11101 tatcttcctg caactttaat attttttcaa aggtttcaat agagttcgtc gcagtctgta
    11161 acacgccggt caggaaaaat gttaaccagt gagggagatt gtgctggagg cgtgtttgca
    11221 tcagattatc atagtataac gaacgattgc gttcaaaaaa atcagataga tataaggttg
    11281 gcccgttgag aattccaaat cgaaccagat acaatgtaat gagaaggcgt ccaagtcgcc
    11341 cgttcccgtc caagaaggga tgtatggttt caaactgata atgggcgagt ccgattttaa
    11401 tcagatgagg tacctgaaga cgtgtttcat ttaggaattt ttcgaggtca ctcataagct
    11461 ccgggacttc cgtatgaata ggcggaataa agatggcatc tttaagcgat gtaccgccaa
    11521 tccaattctg actcgaacga aattcaccgg gcattttatg acggccgcgg acgccttgca
    11581 tgagtacttc atgggtttgt cgaaggaggc gcgtagatag tggcagtttt ttcaattctc
    11641 caatggcata cttcagtgca aagatgtaat tattaacctc cgcccaatcg ttcttttttt
    11701 caggattaac atccgcttct tttaaaaacg cttcttccat ggtcgtttga gtgccttcta
    11761 tgcggctact ttgtgtggcc tcttttacaa tatgcatgcg aataaagaag tccacatcgg
    11821 gaataagccg tgaaaatgca tccaatcgtc ccaatgtgag attagcttcg cttaaaaggt
    11881 tattgagacg tgcatccgat acgacccatt cacgatgaat cgagttaggt aggaagcttt
    11941 tatattcata ttgttggcga aagataccgg atttaaaatc tttaatatcc ataattttcc
    12001 aagttcattt gaaaagtgct tctgcaaata taaaaaatta taattgcaga aaaagcttta
    12061 aatgcaaata taaattttta tatgtgcagg taaaagtaac ctcgtcagca aagcttttgt
    12121 ttttatgtga cttatagcgg agggtcacat caacccctca catcaatctt acccgtcaca
    12181 gcttccgaaa tcaacgccgt ccggtattcc tgtataagct cgatttcctt ctcgatggtc
    12241 gcgatagtcc ggtcgatttt ggcggtttcg gattttagaa aagctacaat gtgattttgc
    12301 tcgtcaaggg aaggcgaggc aatcatgaaa ttttctacaa aattgggcgg aacccttttt
    12361 tgcccagcgg ccccgaccat aaaagcctca ccaaatttca taaacaaatc agtcttggta
    12421 aggaagtata aaaattcgtt cgtggaaaca gctttgacgg gtcttatagt gtgaaattcc
    12481 gttgagccga agccaaaggg cgtttccaac ctgtccaaca aagcaccttt tccattctca
    12541 aagcaaggtg taatcttggc aacgataaca tcattttttt cgaaataagt gtaaccagta
    12601 ctcaattcat ttataggctt tctcaagtct tggataattt gtccatcctc taaaacgttc
    12661 tccattggca aaaataccac caactcgttg gatgacttat caaatacata attgcattta
    12721 gttggattaa cattggcaaa gaactttaac ctcttcacct cccaatgcgc cggaatctcc
    12781 cctaaccaat caatcccgga gggtttcatt ttggctttgg ggtcgatgcc cttggtgacg
    12841 gcgtgattga tgacagcaac ccgttcctcg cggagaagct cgatgagctt ttgtttcttg
    12901 gcgatgaagc cgtcaatctg cgcggtcttg cggtcgagga agtcggcgat ggcggtttgt
    12961 tcggggagag gggggagtga aatcaaaatg tttccaattt gctcggaact tatattgaat
    13021 tgagctgttc ctgttgatga agacattatg ggctcagtaa aacttactga tgtgatgaga
    13081 aaccgaacaa aatccaagct gcaattctgt aacgcaacaa atctaccgac acgctgattt
    13141 agttggcaag gtatgtcaac taagttcaca accgcaaaat tccctaaact accggtttca
    13201 cccaaacttc ctgacattcc aaaaagaagg tcacctgctt ttagagaaaa agcatctact
    13261 gccgctttaa aatcaacacg caccgcatcg cttaaatcca agcgtcctct ttttaggttg
    13321 ttcattttta caatagcgat tccttctgtc ttaaatgctt cagtcttgaa ggcataacca
    13381 ccttggagtg gggcaacgta cttcatacgc gttagctccc atttctctgg aatctctcca
    13441 atccactcca caccggaggg tttgtatttc gggtaggctt tcatgataat attttgcgta
    13501 cttcatttag tacagtatga aatcgttctg tagtgttgag gtagtcaggc aagtctttat
    13561 tcagccaaaa gagtgtttcg actatggttt tgtggttgtt ctcaagactg ttgatattcc
    13621 aacagactgg ctcattgtga aaaactcgat tcctgaaatc acggatgcga ttgagtggcg
    13681 cagatacagt atgacgctga cgttttctct tttccagata gggaaaagcg aggcgtaagt
    13741 ttttccataa aatgcgttcg tactccgtat tgaataaacg cacccaaaat cccaatgtaa
    13801 gttcagcgac cactttagaa gcggtgatgg cttcattgcg catcattatt tgacgttttg
    13861 cattactaat atctttattg agttttacaa gaccggctgt tgtaggaatt tgctgatacc
    13921 agtcttgagt atgaaacatg gtttcgagct cgcggttaac gctattacgt aatgcaactt
    13981 caaaaataga caatgccgga taaaaagctt cagatatcat gatattacat gcataatgct
    14041 caatcgcttt cttgtcattg gccggataag tctcaaaata cttttgagtt cgttcctcag
    14101 aaaacacctt ttgaaaccat gctttatcca ttttatcctt ctatcttgat ttctgggttc
    14161 ataatactta taattattgc gtaagcagtc ccggtccctc ctctcccggt gtttcgatgc
    14221 cggtgcatga agccgggttt ttttatttta tgacctccct aatcatacct tccgtctctt
    14281 tcttcagcgc aagtatatcg gctcggattt ccgacagaga gcgcaagggc ttgtactggt
    14341 agaaatactt ggtaaagttt atctcatagc caatcttggt cttgctctcg tccacccatg
    14401 cgtcagggac gtgcgggagc acttcgcgct tgaaataagt ttctatcttt tctgtaagcg
    14461 gaacattctc ataatcacgg agttcggaat cggctttggg gttaccgttc ttatcggtga
    14521 ggacttggcc cttttcatct ttggccggac gctctacggt gatacgcgcg tagccaaagt
    14581 cttcattgtc aaagattttg caaaattcgc cggatttgaa actgccatag attttagcga
    14641 tggacgtcag ttgcgtttcg ctgataagat tgcgtttatt gccgagactg cgtgtcattt
    14701 tttcgaagaa tgagacggca ttgataagct ggactttgcc tttgcgtttt ttagcttttc
    14761 ggttggtcag aatccatacg tacgttgcga tgccggtatt ataaaaaagc tggtcaggca
    14821 tggcgacgat agcttccagc atatcgtttt cgataatcca tttacgaatc tcgctctcac
    14881 cgctcccggc tgaacctgta aatagcgggg agccattgaa gacaatcccg atacgcgagc
    14941 cgtccttttt cattttggaa atcatgtgca gaagaaacag caatgaaccg tcattgatac
    15001 gcggcaggcc cggcccgaac cgtccggcga aaccgcggtc gtcatgttct ttttctatga
    15061 cttccttctg ctttttccat tccacgccaa agggcggatt gcagagcata taatcaaaga
    15121 gttcgttttc aagtccgtct ttggtaaacg tgtcgccgta tttgatattg ccgggattct
    15181 ggcctttgat gagcatgtca cctttacaga ttgcgtagga ttcgtcgttg atttcctgcc
    15241 caaatacttc cagccgtgca ttaggattga gttcctgtac ataggcctcc gcgaccgaca
    15301 gcatgccacc cgtcccacat gcagggtcat acagcgttct aataatgcct tccttgcgga
    15361 ggatgtcgtt atcgccgata aaaagcaaat caaccattaa ccggataact tcccgcggtg
    15421 tgaagtgctc tccggcggtt tcgttggata attcggaaaa cttgcgaatg agctcctcga
    15481 atacgtagcc catctgcatc gtatcgaggt cggacaaatc ctgtttggca aaatccttaa
    15541 tcacaagata aagcaggttt tctttgtcta atttttcgat ttcatcttca aattggaaat
    15601 aatcgaagat ttccttggcc ccggctgaga agccgttgat ataattgcgg aggttttttt
    15661 caaggccatt ggggtcagcg gccagttttt tgaaatcgaa cttactgcgg ttatgaaatt
    15721 tctgcttagc ggccttattg agaagctttt cgaggatttc cggcttctgt tttttattgt
    15781 cgtcatagac ctgtaaaacc ttggccttgg ttggctcaag aatttggtcc agacgacgga
    15841 gtacagtcaa aggaagaata attttgccgt attcggattg tttatagtta ccgcggagca
    15901 ggtcggcgat ttgccagatg aagttggctt tttcacgatg cgattgaata gtcattggtt
    15961 tttcgaaagt ggtagtttag caaataattc tcgataaagc cggattactt ttttcgctaa
    16021 aaacgattag aaatgaatag tcaatgttac cgaattaaag actatcagtc aatttaatat
    16081 ttataccatt ggcttatact atgattagat acaaagatac aaaaaggtgt acaattgtcc
    16141 ttattgccat agtgtagttt aagccgatac attatttgtt tatcgttatg aggttggcga
    16201 catgatgcaa ttcttcggct ttgatgtgac catagacttt ttcagttatc gcggtacttg
    16261 aatgtccggc taccttggaa acttgatgaa gacttgcgcc gcgctctaaa gcccacgtga
    16321 taaaactatg cctgaggctg tgaaagtgca gtcggtcatc caattccgtc tttcggatgt
    16381 attgtttgag ttttttagat acatattcgt gccggtaggg tttcccgttg cgttgaaata
    16441 tgtagctgtg tttctccatc gtgttttgat atttcatttc taagagtatt agtagttctt
    16501 ctccaatcgg tacattccgg ttttttccgc ttttggtcgt aaagcattcc gtattgcgta
    16561 tctgtatgag acgtttctca aaatgaattt gactccatgt gagatgtacg atttcgctct
    16621 ggcgcatacc gctcatcagt gcaaaaagaa cgagttcttt gaaatccgaa tcatctattg
    16681 ctctcaaaag tttggtaaaa tcagcatgtg taaaataaac gggtgtttgt tctgccggtc
    16741 tgggtttggg gatttttgaa aatggattga cggtaatgta ttcccatcgt ttggcagtgt
    16801 taaatatgct ttttaaggtg acgtaatact ttagcagagc ccacgaattg cactcttggg
    16861 cctttgacgt gaagaagctt tctatatcat gaaccgaaat acgtgataga agagggtttc
    16921 ccatttttcg gctcaattcc ttaagtgccg tgatggccgt gcgcgtcgtg gtcggtcggt
    16981 gattggcctt ggaatagtta gcgtaatcgc aggtaaattc ggataagtga atacatcgga
    17041 tacgttcgct ttgccggtac ttttcgagat tgaaatcttt gagaaatttc agagcatcgg
    17101 ctttggtttt gcttcgtgtg gaaatcttct gctttttgtt gttttcgtcc acgtaccaaa
    17161 gataatacgt gccgttagga tgctttgaaa gaaacatgat aaccctttcc aacgcttcgg
    17221 aaaagtggtt accaaaatgg ttaccattgg ttgctttttt gagctaaatc aggggataag
    17281 cagtaaaaac taaaaaccct taagctgttg taacttaagg gtctttgctg tggctccgga
    17341 cggaattgaa ccgccgacac aaggattttc agtcctctgc tctaccaact gagctacaga
    17401 gccaaattca tatgtttttt tattttcagg caggcaataa tatcaaaaga gaccgaggat
    17461 gtcaagattt ttttcagttc gttgattatt cggacatatg acggcaaaag gctgagaaca
    17521 catttgtttt cagcctaaat accggagagg atccgatgct cccgatacgt tttctaaaag
    17581 attcagattc ccatgttacc cccttggcac gacaggaccc gtgaaaacga tatcggaatt
    17641 aaaagtgata cgtcagattg acttgaggcc cgcggatggg cttatcgcca agctgatagt
    17701 ctttcttgtt ttgttttttc tgaaagctgg cgcccatacc gataaaattg acatcaatac
    17761 ccaaacgtcc gtcgaaaaat aacgccgtca ttcctagccc caatcccatt ttgatataac
    17821 tgcgacgacg ttctaagcta cgattctcgt ttgtattatc gtaaacaccg agatcatcta
    17881 tatccagaaa atagctgcca ccgctgacga cattgacact gaaacgtttg ttgattcggc
    17941 taaaagtgta tttgaaagta aggccggtat taaaatcgct ttgacttttt tgggcaacac
    18001 cgccgatgac aaacagcgcc gagaaatttc taaaaagatc acgttcgtat gataagccaa
    18061 ccccggatac atttcctgca gcaaagccga tacggttttt ggtttcatag taagtgcttt
    18121 ttggctcggg cgtaagtggt tcttcgtttg cgacttcgat cgtttgggcg gtcaccattg
    18181 cattggtcgc gagaacgaat gtgaagattt tcagtaaatt tttcatgacg agactcccta
    18241 aaggtttttg atatgttatt gctacgtgtg atagagatca caatttgtat gccaaaaaca
    18301 taaccttaaa aattaatgct ttattgatgt gcgattcgaa agattaattc acaggagtga
    18361 aaataaaata caagcctgca aggaaaaaga ggcacttggc gggcatttgt atgaaggaca
    18421 gacgatcaga tatgcagaat gttattcatt cctccgaacg ggtcggaaat tttatgcggg
    18481 ttgagcgggt cttcggtcca taaggtatca tccacaaaaa atttgtagtg gtattgcccg
    18541 gctgataaaa gaggtatatc aacacgccat acaccgggac gaatttcaac gaggagtaat
    18601 ttgttctttt gccaatccgt aaaatcgccg acaagattga ccttgcggat attgcgatgg
    18661 taatacaaaa aaacaatgcg atctttgagt atcaatggag actcgtgaat ttccggcata
    18721 gacgtttcta tcaaagcctg ttcgacacaa gccttagctt gtacgaggcc gaatccttgt
    18781 ttttccggtg caataccggg tacacgtttg gccgtcgttt cgaggatcat acgtatcatg
    18841 gtcggcgtca gcaatggatt agcttccagc atttgcgcca ctacggcggc tatgatcgga
    18901 gcggcaaatg aagtgccatc aacatgttga aaacccggtg cgataaattt ttcgccgtcc
    18961 atgcgttgcc gtatagcttt tttgatttct gccgggttga gagaaagaat agccgcgtct
    19021 aaacgcgttt tatgtatcaa cgaccttaac ttacgcttca gattattggc tggagtttta
    19081 taaagtacat gcagaatttg cgattcctca tatgtaaaat tatccggcag catgggtgcc
    19141 ggtaaaaacc gggcatgcgc tacgatttcc ggtttactca gaccatctaa agttatgccg
    19201 taggaggaac catattcgct ggccgtatcc gggttcttgt cgttatgatc gtagatacct
    19261 ccgacggcga tagcaaagcg gcagctcgcc ggtggaatca ccggtcgtga aggattattg
    19321 ccggccgccg caatcacggt aatgcctaac tgatgtaaac ggtgaatagc cttgcaaatg
    19381 cggctttttt cggaaggttc attgcggtca ccacccacgg atacactgac gacgcgaata
    19441 tgatacggct tgtgatgggc gataatccat gtgagggatt taaggatata cgatgatttt
    19501 acttttttac cgtcaaaggc tttgatcaaa acgacattag ctccggatgc gattccgcga
    19561 taataacctt tggaccagat gccgtttccg caggcggagc aggctaccat cgtaccgtgc
    19621 caactggacg gccggattgt gtggaaatct ttttccgtaa acgcttccga tgacacatcc
    19681 acaaatttca gaatacgacc gcgcaattcc ggatgtggat aatatccgct gtccacaaaa
    19741 gctatagtca caccgctacc atcatagtcg gtatgcggcc ggacgcgttc gcgcagagaa
    19801 aggacgggat attttttcaa tcaacggtac actttatgat acatggctga tcaataaaaa
    19861 caattatcgt tatggaaatc aagcggtttt tataaacaca taaaagagat ggacatcgta
    19921 atcattataa aatatattta ccttgatttc ctaaaagcta ttcaatcgta attacaggtg
    19981 tcatcaacaa ttaacctgta taacaaaacc agaggggatt ttatgaaaag tatgagatta
    20041 cttgtgttag gtgttctgac gctgaccatg ttggggtgcg acagcaataa ttccaatacg
    20101 cttatcgctt atgtatatca gcatgagtat ttgggctccg aaggaaatga ttttgactat
    20161 gacggaatcg tcatgggtga tcctctgccg tttatcgaat cggttactgc aaaaaacatc
    20221 accggcgtgc aagagttata tgtttctcaa aattcattcg attttgactt tttgtacgac
    20281 gcaacatttg gcgatggaga tatcacgatc aaaactaccg aaggaacgat aacaggtacc
    20341 gtagatcagc cgacgatgta tgatagtgtc aaagcaagta cgacagatta ttatattcct
    20401 ttgaataccg cgctgacatt gacgacgtat ggcggaaact cagacttcaa atatatttcc
    20461 gcagagttag aatattatga tggcgaatca tggtactatg ttgaaatgga ttcagcattt
    20521 gagggtaata cctttacttt tccggccagc atgcttgata cggagtcgta cttatatatt
    20581 tacggtattg attgcgccaa tggacctatc gctaaagccg gctcgtccgg taatatgaat
    20641 ggggatggat cagggtttat gtggcatatt atttcggatg acaacttcta tatgtctttt
    20701 gccgtcggta tagaagccgg ctccaatgtt aaaaaatcgt cctcgcatgt acaggagcgt
    20761 cgtacacgcg aagatagaaa acgccgtttt caggaaaaaa ctgcaaaaat attgaatgca
    20821 tcggcgatgt agaattttga ttaattctat ttactataaa aaagagccgc taattgcggc
    20881 tcttttttta ttccatgacc aaagtttcgc cgctgtagcc gggcaattgg atgatttctt
    20941 tttcgacgag gccgagtttt ttgagcagtt tgatagaaga agcattatcc ggcgaggtga
    21001 tggcgatgat tttattgagg ccgtgctgtt tttgaccata agccatcgta gccgccgccg
    21061 cttcgtacgc ataaccttgc ccccaatatt ccggtaaaaa agcaaacccg atgtccgcat
    21121 gttcgagtgt gtcacgtttg agcaaaccgc acatgccgat aggtgtatcc gaccctttta
    21181 gagctacaag aaataaacca aatccgtatt tggcatacat cgccatcggg ccatcggtaa
    21241 tataacgtac agcgtcttcg gggctgtgca cattacgatc tccgataaac cgtaaccacg
    21301 atggttcgtt gaccaatcgc aggataaaat gtttatcttc atgggtcaat cggcggagga
    21361 tcaaacgatc cgtttctgca ataatcatag gtttcatttg attgtataaa taagttattg
    21421 actgtcggtt ataaaaaaat caagccatga cagccaccat attcagcaag gggttaagcc
    21481 aaaattcatc accgtcaagc atatggatat atggacgtgc gcgctcgcca ttcagatttt
    21541 tagtaaaaat acgccaagcc gctttttttt tgatacgcat gtgtgcgtgt ggcttcgaat
    21601 cagtaccact gaaaagttcc cacttgttat tatttctttt caaaaaccaa ttagcatcag
    21661 tcaagtcgga tactgaaaac tgtaaaacta tgccgtcggg agccggcata ttacgatata
    21721 catgcggcag cgcacgcata ctggtatcaa gatatggcaa ataccatgcg ttctgcaaaa
    21781 gtgtctcctc atcaccaagc gatgaacgaa tttgttgctg atgatgccat ttttcagtgt
    21841 actcccgagc gacatgaaac caatttttag aaacggattc acccgcccac gcgacgggaa
    21901 atacggcatc atcattatgg cgtatcgttt tcaaataggc acagtaccac gcgcccgtaa
    21961 tttcgagtaa ttcgcatatc atcgccggac tgactctctc aaaagccttg agccagtcgg
    22021 cattgagtgt attaaggaac tgtaccgttg cttcatacga agaaagatcg ttcggtgccg
    22081 gtgcaaaatg atggtcgcgc atcgctgata ctaaacgaat attaccgtca agcagatgta
    22141 gggctacatc acgaaccgtc cacgaaccgg ctgatgtctg tacacgccag tcttccggcc
    22201 ttagatttcg caggcatgcg atgagcaatc ggtccagctc gggaaacaaa tgaaccgtat
    22261 taatttctgt tacgggtttc acgtgataga agcgacctta ggcatatcgt ggtagtttct
    22321 tctttttgat actgcaagtt ttttaaatgc cttcaaacgc gatggcgtaa gttagaatcg
    22381 ccatggcaat ggctccgagt tcaagttcgc gatcatgtac tttgtcgatc gtatcgtgat
    22441 cggaatgatg gtaatcaaaa taacgttggg gatcgggatg aagtccgata cacggtacgc
    22501 ccgttttacc caaaggttcg atatcggttc cgccgtgccc gggcccgacg cgatcggcat
    22561 ctatgatttt gaggagcgat tcaaatcgtg taatccgcgg tagttttacg gaatcagctt
    22621 ctatgccaaa gccgcgcggt gcatgcccgc cgccgtccga ttccaaagcg gcaatatgtt
    22681 tttcgtttgg tcgctcaacc gccgcataac ccttaccgcc gcggacgccg ttttcttcgt
    22741 tcataaataa tacgacacga acggtgcgtt tgggtaaaag gccggttgct ttcatcaatc
    22801 gcaacgcttc gatagagtgt acgaccccgg cgccgtcatc gtgcgcgcct gtacctttat
    22861 cccaactgtc aagatgacct ccgatcacga tcacttcttc cggtttttcc gttccgcgaa
    22921 tttcaccgac gacattgtac gaaagtttat cagccaacgt ttcgcatgtg agtttcattc
    22981 gcacttttac agactgtcct ttggtcagca tagcgctgag attattggca tcctgcgtgc
    23041 tgatcgcagc ggcgggaatt ttcggaatgg aatcgttgta acgcatggcg ccggtgtgcg
    23101 gataggtatc aatccgattg gtcattgagc gaaccagcac ggcggcagct ccgaaacggg
    23161 ccgcttcgac cgcaccgtat ccgcgttgat ctaccgcgcc gccgtacgct tcaaaaggag
    23221 caaataacgt tttatcaaac gggcgattgt aaaatacaat tttgcctttc actttgtcac
    23281 caagcgtacg cacttcctga agacttttca cttcgatgat ttcggactcg atgccttctt
    23341 taggcgtggc aatactcccg cctagtgcgc agatattgag cttttgagag ggggcggttg
    23401 atgtgataaa ggcttcttcg acaggtccgc gtacccaatg cggtaccatt acgggttcca
    23461 agcgaacatt ttcaaaaccg agatttttca ttgtacgctc gccccacgca acggcactgt
    23521 ccgccattgt agaaccgctc agtcgtttag gcgctttcgt cgtaagctcg gataacatcg
    23581 tgtacgcgtg aagatcattc aaaccttgtt tcatcaaagt catcgcggcc ggttccaaat
    23641 gtttgacatc cggttgtttc agagcaggtt tttggcatga tgcgaccatg atgatcaaaa
    23701 aaacacaaaa ccattgtttc atacgactaa tcctgtagtt aaacgataat ggaaatttat
    23761 ttgattacgg tacgattttt ttcgatccaa tacgcccaca gtacaaataa ccacatcagg
    23821 tttccggcaa gcgctatgcc tgtaatattc ggcggcggag ggccgagaag atttcctgta
    23881 tgaatgatca taagaaaaac aatcaaaccc caaaacgccc atacgcctgt ctttccggta
    23941 gcacgagtgt tgcgatgata gataaaaatt cctgcagcca ttaatgccaa ctctgcaacg
    24001 acagcaaccg gaggaatatt ccaccagccg aggccaaaca tctgcgaact gcccggcgaa
    24061 agcggtaaat ccggtcggtg cgttacaaaa tccaaaatcc agtgactgca tgccgtgata
    24121 aaaagcaacg cggccacgcg ttgatcgcga tatcgccgaa gcgcccaaag agcgaataga
    24181 gatgcaacga gaagattggt catgagactg tgcgaatatg gatagtgatc aaaagccagc
    24241 ggcgtcatca ctgtattgcc gggttcgatg cgtaccgatt cccatcccaa taaaagcagg
    24301 acaggccata tcaagtccgg ccattgtacg gcgataaaaa gtacgggaag tgaaatatgc
    24361 ggcatccatt ttttggaaat aaatcccgtc gcaaaatgcc cgatgaacat gtctgctcct
    24421 ctaaaaaatc tgaatacgaa gaatacttat taacgtgtaa aagcttcgat aaaccaattg
    24481 acaagccaaa tcgtcgcgat cccgccgatg gcaataagca gtatgccgat ggctgtagaa
    24541 atccaacccg accaaatgcc gatctgacaa tatgtttcgc cgttggtcga atatctttca
    24601 ccgcgcagtt ttttgagttc attgtttccg atgatgatag ccataatgcc aggtagcggt
    24661 gcacagatca cggcgaaaaa aacaaaaaac aacgaaatca atccgagcgt catggcgagt
    24721 tttgccatac cgctggccgg atgatcttcg cgatgagtga tgtggtcttt tggaaatcgg
    24781 gattcgcgcg caggttcttc tttaacaggt attgtcagtt ccgcgccgca actgacacag
    24841 taggccgacg tgtcaagatt gttggtcgtg cagttaggac agattttcat gagtatttcc
    24901 gaaattgatc tttacaatat acgcgggaaa tgatgatcgg ttactttgaa aataaaaaag
    24961 ccgcagcata atctcgatgc aatacgaaaa ctatgctgcg gctgtgaaat gagtactact
    25021 tataagccgg aatgcccgtc acgctttcgc cgatgatcaa cgtatgaatg tcgtgcgtac
    25081 cttcgtaggt tttcacggat tcgagattca tcatatggcg tatgatcgga tattcgtcga
    25141 tgataccgtt agcgccatgc agatcgcgcg ctatccgggc tatctcaagc gcgatataca
    25201 cgttattgcg tttggccatc gaaacctgat gaaatttcat cttgttgctg tctttcaggc
    25261 gacccagttg caataccaaa agttggcctt tggtgatttc agtaatcata taggccaatt
    25321 tttcctgtac caactggaag ctgccgatgg gtttatcgaa ttggatacgt tctttagcgt
    25381 aacgtacgga cgaatcaaaa catgccatcg cagcgccgag cgctccccac gcgataccgt
    25441 agcgcgcttg ggtaagacat ccgaggggcg ctttgagacc ggaaccgtta ggtaaaatat
    25501 tttcttcagg aatgcgaaca tcctgaagca caagttcgct ggtcacggat gcgcgtaatg
    25561 aaaacttacc cttcatttcc ggcgctgtaa atcccggtgt tcctttttct acgaggaagc
    25621 cgcgcaccac accatccaat ttcgcccaga caacggccac atcggaaacc gtgccgttgg
    25681 tgatccacat cttggcgcca ttgaggatat agtgctttcc gtctttgacg gctttagttt
    25741 tcatgccgcc gggatcggag ccgttatcag gttcagttag accgaagcat ccgatttttt
    25801 cacccttagc cagtttgggt aaccaataat ttttttgttc ttcggaaccg taggcgtaga
    25861 ttggatacat tacgagcgcg ccctgcaccg atgcaaaact gcgcaccccg ctgtcaccgc
    25921 gctccagctc ctgcatgata agtccgtacg cgacattgtt catgccggca caaccgtatt
    25981 tctctggcag attggctccg aaaacgccga ggtcggccat ttcttttacg agattcatcg
    26041 gaaacgtgcc gttgcgatta tgctcttcga tgatgggcag gattttgtca tctacaaaat
    26101 cccgaactgt atcacgcgtc atccgctctt cgtcggagag caacgtatcc atatcataat
    26161 aatctacatt cttaaacttt ccagccataa aactccttgt atgcattcac ttaaaattaa
    26221 aacgtcacgc cgagaaccat cgccttggtc cagtttccgc cgatattggt agcgcggatc
    26281 agtccgaaag cgctcacacc gaggctggct tttgcgccga attgcaaatt gaatccgcta
    26341 tccgtagctg aatcgctgcc tttcggtttg ttgtaattga gcgtatatcc gccgcccgcg
    26401 accaattttg ccacgccgac gccgatgaat gtatattgca catccgcacc ggtattgatg
    26461 cttgttgcgt tatctacgaa aacgtattcg gcgttaggaa cgaaggtcaa aggtaatacc
    26521 ggcaaactga cttcgatatg tccgccgaca aaataatcgc cgccgtcacc gatattgacg
    26581 cctgcgcgcg gtcccatttt aacaggcaat tgagcgttgg cattttgaga aaatgtaaat
    26641 gcagtgatca gtgctgcaat catcatccat tttttcataa aaaactcctt taaatttata
    26701 ggacaattca tattccgtgt atttcatccg aacgcggcaa gataaactca aaaaatctga
    26761 atttcaagcc attcacgagg actttatttg ggccagagga cttccattcc gttttttccg
    26821 ttacccacgg cttcgggatc catgggcgcg atacaccatg cgccgacgag ataccctttg
    26881 tcttttttaa aatctttata cgacaccgtt ccgtaaatgg atttatccac ggatacataa
    26941 ctgacagaga gaatattatc aatgtggatg ccgagtccgt aatacgagag attaccgttt
    27001 tcaaaccaac gtaccatata ataatcgccg gttttttcga tcgtcacatc aatcacataa
    27061 ctcggttttt ccgtatattt ttcgccaggg cgaaatcctt tgccggtata tgaacctgtg
    27121 aaagggtccg attgtgcatg gatggctgtg gccatgagcg acaaacaaaa taaaactaaa
    27181 atccgggaat ttttcataac acctccggtg tttgttgagt aggaaaaata agcggaaaaa
    27241 atcaatcgtt caaaaaccaa ttcggatcaa tcagcgcatc atcaaaaatg tgatcttgcg
    27301 atgtgatttc gcgtaaccag gcgcgatcgt ccggatgcaa tgcggaaccg ttacaatgat
    27361 attcggacag acgtgctaat ttttcaaaag cggtatggtg ttccataaag cgctcggtcg
    27421 cataatcaat cgccgactgc gtgctgatca aaaaaggcca atcggaagat tcgagaagaa
    27481 aaagttcacg cgccatttgt ttgatgatcg cggtccattc gggatcggta ttattgttcc
    27541 atcgctgcac atgatcgcgc atagccgctt cgtcgttgta gataaaaggc cacatccaat
    27601 ccacctcatg attaaaccac acatagtggt tgccacccgc gccccaactg ccttccggaa
    27661 tagcgatgat tttttcaggc ggattctgat ccaacgattc ggcgcaatgc atagtttgga
    27721 ttgcgggatc gtcatccatc agttcgaaga tggttttgag aaaaccggga ccttcaaacc
    27781 accaatgccc gaaaagttcg gaatcgaaag gagcggtaag cgtgccttcg tatccgtgtt
    27841 ttgttttaaa tcgagtgagt gcctgacgca cggcttgcac aaaatgctgt gcatcatcgg
    27901 cgatggccga ttggattttt tgaggttcgt acggtgcttt tagaccgagg tcggcctcaa
    27961 gactagtgac gcgccagtag cggtgtccgc tttgctcgtg ctttttatga aaatccaaat
    28021 accattcgcc gccgggataa ccttgttcac tggaccaaac ctgcatggat gtttccatat
    28081 cccttgcaaa aatggaaacg ggattaccgg gtgcgtcgta cgatgatttg gttttataga
    28141 catcatatac cgattgcaga aaatccgctt taggtatcgg gcgattttca ctggccaaac
    28201 gcatcatcag tttgcgtaga ttttcaaacc gtcccatgta catcggtacg gcccgacccg
    28261 gctcgacgag cgaggaatca acgataaaat attccaaact gttggcgtct agtatttcct
    28321 cgataccttt acgaagacgc ggctcggagc caagcgccga agccacgggc ggtgtccata
    28381 cataccgggg gcgataggcg cattccggaa gccatattcc gcgcggttta cggccaaaat
    28441 atttttcatg cgtcacgacg gccattcgga tttgcgcgtg gatattggca tcggtaccga
    28501 ggagaggaaa atatccgtgg gtagcaccgc aggtgatgat gtcaagtacg ccttgatctt
    28561 gtaaggtgcg aaacgcgccg attaaatcat ggcggtatgt gagtgtaaaa tctttgagcg
    28621 tattttgata aaaatccaac caatattccg ccagcggaac caactttttc tccgtcgggc
    28681 ttttttcaaa agacaccata tcggtgcggg cgcgatcaat ctcatcatac aaaaaagcga
    28741 caaattcttt tttaaacgtt tcgctcgcaa gttgttcggt aatgaccgga gtcatgctga
    28801 tggtaaggcg cggccggcgg ccttcgtttt gcaatgcatt cagggcattg agcagcggaa
    28861 tataagtttc ggacgcggct tcataaagcc actccatgcc atgcggccat tgaccatgat
    28921 gcagcacata gggtagatgc gtatgcagga cgaggataaa attaccgacc gatttttttg
    28981 tcatggtgcc gcgtaccgcc gtaaaaaacg attgttttga atatagagaa gcaaataggg
    29041 ggaatcaagg gaattggcgt atgaaaatgg ccgaacacaa aaaaaccctg catgccaaag
    29101 cttgcagggt ttaaaaaaat ggaggttgct ttatatcaga taacgccgat ttttttacct
    29161 tccgtgtaga gcacgtccaa ggcgtgatct aaatcctggc gcgtatgttt ggcgctgatc
    29221 gtacagcgaa gacgttcctg gccgcgcgca acggcaggga aaatcatcgg cagcatgaga
    29281 atattgttct cgtacaattt tttggaaatg tacatcgttt tttccgagtc accgatgatg
    29341 atcggtacga tcggtgtttc gctgtcgccg tagttaaatc cgaattcttt gagtttggat
    29401 ttgaagtacg actcgttgac gcgcagattt tgaagatgct cgggttcaga acgaatgatg
    29461 tctatgattt tggatacgga aacgatattg gaaacggggg atgcggcgga gaagatgagg
    29521 ctgcgggctt tatgtttgat gtgggagatc acatcacgct ggccacacac aaagcctccg
    29581 acgccgccta gggctttgct gaaggtaccc atcagcagat caatatcttt gtccatgccg
    29641 aagtgttcac ccgtgccgcg tccggtttta cccaaaacac cggtgccatg ggcgtcatct
    29701 acggcgatac acgcatcgta tttgttggca atattgacaa aatccggaag acgacaaatg
    29761 tcgccgtcca tactgtaaac accttctaca atgatcatca catttttacc gcgtttgaca
    29821 ctggactcaa cgatggactc taagcttccc ggatcattgt gtcgaaaata ccgtatctgt
    29881 ccgtccagat ttttggtact tccgccgcgc gtcatgtgag tacctacggc cagagcgcat
    29941 ccgtcaagga tacatgcgtg gctcagtttg tcaatggcca acacaatatc atcttcgatc
    30001 gaatcatcgg ccttgaccag catggccgat acggtgccaa gcaaagtctg ataaccggta
    30061 ctaaatacaa tacagttttc tttttgcttc caatcggcaa ttttttcttc caagcgcgta
    30121 tgttcgatgg tcgtgccgac gacatagcgg gatccgcaaa gtcccgatcc gtaaatatcc
    30181 agcgccttac gtgcttcttc gattacgcgc ggatcatgcg taagacccaa ataatcgttg
    30241 gatgaaaaat tgacgagatt ttgacctttg acggtgatat atgtgccggt gcgttccagt
    30301 tccgtaaagt aactgtataa gcctttggcc ttaatatctt cgacaaagtt ggaaaacggt
    30361 ttacattttt cgactaacca cgtactcatg ccctgctccg gttattggat gacaccctca
    30421 acgatacccg aaactatata caaccaatat aagtcaggta tatcaaaatt ttgagaaaat
    30481 acacagctaa aaataaaaag taaaggagtt tctttgtctt aaaacgatgc taaagtcttg
    30541 aaaattcttc acgaagcata tcttttcaat tgacaatctc cattcgcttt tcatatattt
    30601 ccccgctatt taatttatct tgtaatttga aagtctgaaa gaatacccga ataatcatta
    30661 cgcaatacat tgcagtaaag attccaatca tcggtcgcaa acataaaatt tcaaattatt
    30721 aagcttgtgt tttctgttaa accgatgctg ttcggttttg taattattaa aacaaatgtt
    30781 atttcaatgg caatctgagg ccttatgtcg ggtactttaa agcgaatttt gattgtagat
    30841 gacgaagaga cgcttacgtt tagtctctac cagagtttta tcatatccaa agaaaattac
    30901 gaagtcgtga ccgcttcctc cggcaccgag gcttgggaaa aatttgccga acatccgttt
    30961 gatctgatat tgacggatat taccatgccc ggcatcagcg ggatcgaatt gctcaaacgc
    31021 gtaaaaaaag aacgcccgcc gacgcatgtg atcatcatga cggcatacgg ttcagatgaa
    31081 aaaaaagagg aagcgctgag ttttggcgca taccgctata tcgaaaaacc gtttgaaatc
    31141 aagatgatga aaagcgtaat tgccgaagcg ttaaaataag aataaagggt attgacgtgg
    31201 cgcttgtagg aaatctaaga gatctcaaat tgaccaacat cgttcaactg aactgtatgg
    31261 aaaagaacga agcgatgttt acggtcgaat tacgcaatgc gatcggacgt atttattttg
    31321 cggaaggtaa tattgtacac gcgacatttg gtactgtaga aggtgaagat gccgtatata
    31381 aaatattggc catcaaggaa ggccctttta aagtcgaaaa cggcgttgct tcgccgaagc
    31441 gtacgatcaa tgcgccgtgg ggcaatctga tgatggaagg ccttcgtctg atagatgaag
    31501 ccttagaggc caaaggcgaa gccatggcca agtttacccg tgacatcaaa accatcaacg
    31561 gcgtaaacgc cgtgctggta tcgtcaccgc agggcgagat cctgatggaa gacggtattg
    31621 ccaacggaaa acgcacctct gccggtatgg gttttatcat gcgcaaactg gctaagatcg
    31681 gccgtgcttc caaatcaggc acgtataagt ttggcatcgt aacgggcaaa aatgacagaa
    31741 aaattatttc caagtggcat gacgatatta tcacgctgac cgtagagcaa aaagcggcag
    31801 cggatctgat cgagcaggca atagaaaaag cgcccggtat tcaaaaagtt tccgaataag
    31861 cgttttccac ggacaggatt atgaaagaca aactcgaaga aatcaatagc gtcaacggtg
    31921 tcaaagcctc atttatttat tttaatgacg gcgaaacggt gtatgctaat atgccgcaag
    31981 gtttcgacga agcgacatat gtggaaatcg cgcgcgatgt aattcaagtc gccgccatgt
    32041 ttgaccgcct ctccacgccg ctgcatgaat acgatctcaa atacgataat gggcgcgtca
    32101 tcgcctatcc gatgagtaat tttaacctga tcattctgtg tcagcccaat gtgagcgtgg
    32161 cgatgttgcg cctcacagtc aatgtggcgc tggcggatct ggaagccgat aaaaaatttc
    32221 aaaaaagact cagtaaagtt gaagcgtccc gccgcagttt tctggtgcgg agtaatatgg
    32281 atgcggaaag ctgggcgctg gtcgaagttg tcaattaacc aacacgtgcg ggcctgatcc
    32341 atggcaaccg ttctctccgg aaatttatct cacgttgacg tgctgagcat tgtcaagtta
    32401 ttggtcgcag gcgaccgtac ggggcgcatt ttgctggagc gcggcgacca acgcgaaaat
    32461 ggggaaattt atctggccaa taaacgtatc gtccatgccg tgtgcgatac ctatctcggc
    32521 gagcccgcct tccacgagtt gatactctgg accagcggta agttttcatt tgaaccggat
    32581 gtggcctcgc tacagaaaag cattgataaa gaaacaggca agctcatcac agaagcgacg
    32641 ttagagtatg aagcctggca aaaaattgca cgccacatac cttcgttccg cgtcaaattt
    32701 cgtaaaacgg ataatgagcc ttcgccgacc attcgtctta aagctaaaga ctgggaattg
    32761 ttacatctct tagaaaaaga cgaatactcc gtggatgagt tatcctcgcg tcttggtatg
    32821 aaagatatgg atgtggcggg gatcgtatac atgctggccg aagccggatt gatcacggcg
    32881 gtttccggcg ctacggcgtc ggccgataaa caaaatgtat cttctgcatt ttttaaaaat
    32941 ctggaaaatg aattgattca attgatcggg ccggtggctt cgatcattat tgacgacgtg
    33001 gttgagacgt ttggtgaaga tcagaaaacg tttcccaaag acaaagtgcc gccgctggtg
    33061 gaggcgatag ccaatgagat atacgatccg gccaaacaat tggcct
//
LOCUS       DAOQNQ010000007        31101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_459_length_31101_cov_6.338852, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000007 DAOQNQ010000000
VERSION     DAOQNQ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 31101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31101
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_459_length_31101_cov_6.338852"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2324)
                     /locus_tag="PK754_00995"
     CDS             complement(<1..2324)
                     /locus_tag="PK754_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB08110.1"
                     /translation="MKRVILILSVLCIAHLGAQEKGTIKGKVLDAVGKQPLAGVSVRI
                     VGSTTGAISDINGVYTISGLSENVYKLQLDYIGYQSHFETDIRVVRGKITYVREIEMQ
                     ETSIAGEEITVEASGFEQDKSAPVSNYNYSREEIRRSPGATGDIFRAIETLPGVSSSG
                     GEFSSFSVRGGSPRDNIVLVDNIPFSKVSHFDGGTEEQEAQGGRFSIFTPGLIESANF
                     QAGGFGARYGGKNASLVDLHIKEGNRETPTVNGTYDAIGWEINYDGPSYIHKKTSLLL
                     SARHQDFNNILKWTGQKDLGHPRYTDIIVKTTTDIASAHKLSFLGIYADDLFDRNLSH
                     VYESNNLYGNDLAEGGETKYLIGLNWRWLTGKKSFMESVVYYGHRENPFKSGVAYTDA
                     TNGIAPAQSEVRTRDNIYVYDFNEQQLGAKSQFTYQVFTHLTAMTGLEINRFSMDNTL
                     KQNGWDTTFVYDKDDYRSDISQLYLVRDPARTSYANKQTRYQSAGFLELSYSPDKKIT
                     LNPGVRYEYSTFNRRHAISPRVSISYVLNARTTLNGATGLYYQAPELDVTGSDARNKS
                     LKNQKALHLIAGGTRYLGNDVKLTIEGYYKKLDHLIVKPDRTSNLRTNQGEGWARGVD
                     IGLVKRFAQRWYGQINYSRMASRRNDHNGEGWYNSDFSQPNIFNVLFGYEFNKEWSFS
                     TKWKYATGRPKDTYVIHENVFNDTDHMRYSKEIIRNNGDRLDDFHTWNVRVDYRKQIG
                     RFAVVSFVDILNMYGHLNVNEEFFQYQNGKLDKRG"
     gene            complement(2432..3076)
                     /locus_tag="PK754_01000"
     CDS             complement(2432..3076)
                     /locus_tag="PK754_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNB08111.1"
                     /translation="MARPKESERQDIKALVLRHSKALFLKQGFANVTMRDIAREIGYS
                     PAAIYLYFDNKDQIYYELFNEGFQILYRRQLETAQQSFNDPTDESYSMGQAYIRFAMD
                     FPEYYDLMFVMREPRKFMEHCLNENTGEPIRTDFSEMSYNLLRATIKKCIDQGYFKGF
                     DVEIASFTTWAAVHGMATLILRGMLVAPEEMKPIIFNGAHECLQALIKTSKQTK"
     gene            complement(3284..3418)
                     /locus_tag="PK754_01005"
     CDS             complement(3284..3418)
                     /locus_tag="PK754_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08112.1"
                     /translation="MRFDVVDMGSFPSANITKTPLKVNEDGLKKKQEFLGSISSKKII
                     "
     gene            3396..4781
                     /gene="rimO"
                     /locus_tag="PK754_01010"
     CDS             3396..4781
                     /gene="rimO"
                     /locus_tag="PK754_01010"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNB08113.1"
                     /translation="MSTTSKRITLPVLGEASRQRAPRSNKQKKVNIVSLGCPKNQVDS
                     EVILGNLKHTRIVDKAEEADTIVINTCGFIESAKQESIQTILEAIELKKRAAKKGITK
                     EIIVAGCLSERYPKELAAEMPEVDAFFGVHQFDKITERIEGHYQQVLFTERILLNQRH
                     YAYLKISEGCNQRCGFCYIPMIRGNLDSKSIADNVAEAEKLASLGVKELICVSQDTSS
                     YGYDFDRSAPNLRQNKHLIELLHALGEVRGIEWIRVMYLYPSLFSDTLIETIATHPKI
                     CKYIDMPVQHISDRILKTMRRNTGKKQTTDLLYKIKARMPDVALRTSMIVGYPGETEA
                     DFEELCDFVREFKFDRLGVFIYSQEEGTYAYDLEPQVEEAVKKERFNRLMEIQREISH
                     AHNMEKIDQTFRVMIDTQSSEHYIGRTMSDAPEIDNEVIITSSRKLKPGQFVDVKITD
                     ATEYDLYAEAL"
     gene            4860..6200
                     /locus_tag="PK754_01015"
     CDS             4860..6200
                     /locus_tag="PK754_01015"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNB08114.1"
                     /translation="MKRILSLIVLTFLGSCALRGQANMGKTGGGPFFFLDAANVAAKD
                     TTKGRVEIFIKIAYSELLFARYNQVLFRSQYEVNYTLADKKGNVVLREIQDKEIVTEH
                     YEETLSDLRFHFSRLTLNIDPGEYILTVVITDKETQKFGQRKLSLPVRTFREKSIGIS
                     DLLFADKIQKDSVDDIIHILPNVFKSFDNEFKKYAVYFEIYNSKYAWKNRDSIGNLPE
                     EKEPVRIRYRVYDKNQKIVTEDSTVREVNQFQTFSSIEIDKSKVSFGKYVLDVMVYGK
                     NGVRATSKAIFDVRLSTFTAPSLSATAFDLDVAIKQMRHVGRNLNTDKILKGTQKEKS
                     DFFENFWRAKDPTPGTERNEIMEEYFRRVDYANRYFTSGYREGWDTDRGMVFIILEAP
                     DDIERHPFDNNDKPYEIWYYYQANLRLLFVDVNQIGDYELMNRQEFESYLYLRR"
     gene            6205..7131
                     /gene="era"
                     /locus_tag="PK754_01020"
     CDS             6205..7131
                     /gene="era"
                     /locus_tag="PK754_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNB08115.1"
                     /translation="MNETNNLGEPTSAMIRCGYVAIIGRPNVGKSTLMNQLLHVKLSI
                     VTPKPQTTRQRVLGILNEPGTQAIFLDTPGLIAPQYALQTAMMKIADQSMEAADVLLI
                     MIDGTSKAESDFVQELFKTKLLHSDIPKVLAINKVDRMAKPELLPMMDTYIKTGFFKE
                     VIPISALENDGVEQMKQALFKYLPLNPPFYPPDALTEQPERFFVAEIIREKIFLHYTQ
                     EVPYSTEVIIEEFKERERGKDLIRAIIIVERDSQKAILIGKKGEALKRIGETARKDIE
                     AFLDRQVFLELWVKVKEEWRSNDAMLRSLGYR"
     gene            7128..7550
                     /locus_tag="PK754_01025"
     CDS             7128..7550
                     /locus_tag="PK754_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08116.1"
                     /translation="MIRFFWIMVIAVFPLIQTCTQPNEDSPSRRVRQFTRSYFHRKWD
                     AALVHCTDAARSKILNESSFERQLPDIWKEPEISISEFYVKDSIPDPTGTMIYDLVLL
                     DRDATGGFQESLFRITLKSDSGGKISNYQFIRSDYDIR"
     gene            7537..8769
                     /locus_tag="PK754_01030"
     CDS             7537..8769
                     /locus_tag="PK754_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNB08117.1"
                     /translation="MISVDEALHIILSKAKPLAIRTVPFKDACGYALAENIVSREMIP
                     PWDNSGVDGYAVIASDTSKTKQPCMLKVLDTVHAGDVSTKKLTAGTAIQIMTGAPVPR
                     GTTAIVMVEHTRRVGQWVHIQKPAREGDHIRKAGSDIQKNQIVFSRDHIIRPYDLGVL
                     ASIGKSKIRIQTKPTIALLATGDELLSVEEKLKPGKIRASTHFALTALLQKAGYPVIN
                     LGIAHDTPKAIAAQIQKALKADVIITTGGVSMGEKDYVRQAVEKAGIKIHFWKVRQKP
                     GKPMVFGSKGKKLFFGLPGNPVSSLVCFELYVTPAIARMASQPSPILRIPVRLDNPVS
                     KKPGLRHFLRARLYIHDGQIKASLSGEQSSGVLSHMAYAQALLDIPENDGDKNKNDTV
                     DAVVLDRDHLNQLIISIL"
     gene            8773..11163
                     /locus_tag="PK754_01035"
     CDS             8773..11163
                     /locus_tag="PK754_01035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HNB08118.1"
                     /translation="MTTHFDIQPLEFDKLKELIRQCVLTPMADACVQAMKPMTDADLI
                     EKRLDMLVEMADLLRYDDTFPIDTLTDIRQLLDRIHSHGTFLPSSDLLLVARFVETTR
                     KIKNYVRHRHEKYPLLSRDTDTLHDLRTIEDAIQHAIDDHGNIKDHASPKLRQLRRDI
                     DNKSHSLRKKLESLARQFTDAGYSQEAIVTMRDGRMVIPVKDEYKNTVRGFVHDTSAS
                     GQTVFIEPAEGLELNNEMRRLLIDEQREVERILIEIADTIRADRPALYENIDIATMIE
                     FLFAKARFCNTLQGIKPKLNTSGYIQIKKGFHPLLLHKENQKMPDQRRPVIPLDIEIG
                     KNFRTLIISGPNAGGKTVALKTVGLFVLMTQCGLLIPCGFDSELSLFTRLFADIGDDQ
                     SIENDLSTFSSHMQHLSEMASQLDDQTLILIDEMGSGTDPKEGASLAIALLEYFNNHR
                     AVSIVTTHHGELKAFAHNTPGVENGSMEFDQATLEPTYVFRPGIPGSSYAMEISRRIG
                     LHPDIIERAKSLSGTEALRLETLIHDLQSRVKKYETDLADINREKTGLEGMVKFYNDR
                     SKELKQKERALKQTILEEKQRVLAEANKKIEATILEIKKAEASKEAIKSAKTSIQNER
                     LATDQALADEKKLTEFVSEPLHVKKGDRVYIRHMDIEGTVIESPENGGQALIAVGAIR
                     LKIDPRQLSPAQNAGEKNSSPARQTIQWDTDDIGQELDLRGLTAEEALYEVDKYLTDA
                     GILGFREVTLLHGKGSGILRNRINEFLKRDRRIDSYRLGQYGEGDTGVTVVVLKND"
     gene            complement(11170..11718)
                     /locus_tag="PK754_01040"
     CDS             complement(11170..11718)
                     /locus_tag="PK754_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08119.1"
                     /translation="MMTTKPSLKRQMVGILDQFLRGVISARELRSAALPLLQISAHKR
                     GRKNYIERYLVDISGKKDSELTRDYIYELRDMIIGDAVTSEKDRGRVFKRTLRKLIER
                     YFYEEIEEAYYLSLLYDLMFEFDSEVEREPAVKNFFAQIQSATPIHDDQMTVIEIAET
                     QDVIYDKTMDFYEEYFKSLWDD"
     gene            complement(11781..12782)
                     /locus_tag="PK754_01045"
     CDS             complement(11781..12782)
                     /locus_tag="PK754_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08120.1"
                     /translation="MKKSIVLVLIGCLMVLWGCDDNNPYSPRLVLNLTDVYPDILYVS
                     EIFGSQHINAIHDTARGDILDISVNDSELDPTWSADGRKFAYTNIHLFNQSTGQPLHS
                     NIYIRDMDSSTLTPVTYSKYYLDTNFTFQGIVLNMRPDWSLMTNKMVFISNRDSTFDI
                     YTTTITDTLYGDTTAVRLTDETDKLDMFCHPSWSPDGSKIVYTSSKTGNEEIWIMNSD
                     GTGKTQLTDLNASIAGRPRFSPSGDKIAFYSSSSKNGNDSLNIFTMNPNGTGIQKVTQ
                     SGNNIDPAWTPDGNRIVYSKRTSSRGYIYLINRDGTEEKKMISKDSRAYYPIWRPAH"
     gene            complement(12802..14106)
                     /gene="asnS"
                     /locus_tag="PK754_01050"
     CDS             complement(12802..14106)
                     /gene="asnS"
                     /locus_tag="PK754_01050"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNB08121.1"
                     /translation="MRSRVYIEDVFKGKFSDGEEVTIKGWLYNKRSSGKLNFLLVRDG
                     TNTIQATVYKPSVTPESWEAADSASQESSLSVKGKVKKDERAPGGYELQVTEVVLLGY
                     AKDYPISPKEHGIDFLMDHRHLWLRSKQQHAVLRIRHTIIKAIRDFFDGRGFTLVDTP
                     IFTPAACEGTSTLFETDYFDTKAYLTQSGQLYAEAGAMAFGKVYCFGPTFRAEKSKTR
                     RHLTEFWMVEPEVAFNDLYDNMELAEDFLEYIVQTVLKDRMEELKTLERDITKLECVK
                     APFPRVHYDEAVKILKEANQPFEYGNDFGAPDEAIISAKYDRPVIVHHYPSAVKAFYM
                     KRDPNEPDKALAMDILAPEGYGEIVGGSQREDDYEALLSRIQEHKLPVEAFDWYLDLR
                     KYGTVPHAGFGLGVERTVTWICGIQHLRETIPFPRMIHRLKP"
     gene            complement(14145..15557)
                     /locus_tag="PK754_01055"
     CDS             complement(14145..15557)
                     /locus_tag="PK754_01055"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNB08122.1"
                     /translation="MKHRILFVCCVMLWASMTQINAQKGSGNQSSGRLSFYVDYAAFK
                     MFNQKNVTYTQLYFYMSRNQFNFVKKDTSYSAAYTVSLEFTDLNDSKNVVQNTWTTVM
                     DDRIAEADTGKEVPLLFEYPLLLKPSKYILKATIQDKNDASRSGSYTESIEINDFDKS
                     DLMISQIQFATQALKGGNSGEMFVKNGYTVYPNPTRFYGSNLPRLSFYSEVYNLKHTP
                     DDATTNTYTAEFIVTDENGSIIKEYPSRQINKAGETSLLMHNVNVISLPSGRYVFMVR
                     ITDNASKSVAISKKLFVVYREGESIYETQADDSFFKDLDEAGAIRAGNIVSLIGREDE
                     KKIFSQLEFEGKKKFLDRFWKDRDPSPGTKSNEKLMDYYKRYEEANMKFTTPNVQGWK
                     TDMGRVMVVYGFPAQIEKHEFESDMKPYQIWYFLQLKDQPAQTIFVFADMDESGTLRL
                     IHSNARGEIASPNWQTMIKK"
     gene            complement(15615..16196)
                     /gene="yihA"
                     /locus_tag="PK754_01060"
     CDS             complement(15615..16196)
                     /gene="yihA"
                     /locus_tag="PK754_01060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNB08123.1"
                     /translation="MIIKSVEFIASVYKPEQVPKPQLPEIAFFGRSNVGKSTLLNTLT
                     GKKMARVSSTPGKTQCLNYYKINDAFYLIDAPGYGYAKVERKLREAWQRNLGAWLTEN
                     DRIRLAVLIIDARIEPQASDLQMAEFLNYYQVPTLIAANKADKLKNSERKPILKLLNE
                     AYGTWEWPIDFTSGETGEGKVAVLKAMDSALTH"
     gene            complement(16293..17693)
                     /gene="lpdA"
                     /locus_tag="PK754_01065"
     CDS             complement(16293..17693)
                     /gene="lpdA"
                     /locus_tag="PK754_01065"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNB08124.1"
                     /translation="METKNFDVVVLGGGPGGYVAAIKAAQLGFKTAVVEREKIGGVCV
                     NIGCIPTKALLKNAEMYEHMKHAAEWGYSFDNLKYDYKAIIKRSRDVADANSKGGDFL
                     MKKNKIEVIKGTGKFLDKSTLSVADATGKETTQVKGKHIIIATGARARMLPNLKADGK
                     RILTSTEAMTNTEQPKSIVIIGAGAIGIEFAYFFNTFGTKVTVVEMMPTILPVEDEEV
                     SNTLLKVLQKKGMDIHTDTKVEKVDVGANNVTVTVSSKGKTQTITADQCLVAIGVQGN
                     TENIGLEKIGVTVEKGWIKVDEYYRTNVQGVYAIGDIIGAPWLAHVASHEGIVCVEKM
                     AGKDTHPIDYSSIPGCTYCQPQVGSIGLTEKKAREKGYDIKVGKFPFSASGKARAIGE
                     RDGFVKVIFDAKYGELLGAHILGSEATEMIAEYGIAKSLESTYKEIGNTIHAHPTLSE
                     AMMEAALDAYKEAVHI"
     gene            17809..18030
                     /locus_tag="PK754_01070"
     CDS             17809..18030
                     /locus_tag="PK754_01070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNB08125.1"
                     /translation="MPIYEFTCKKCGTDFSELFHSSTFDASEVECPKCRAHEANRKLS
                     VFAADCKSSGESMPMMGGGCGAGCGCHMN"
     gene            18078..19574
                     /locus_tag="PK754_01075"
     CDS             18078..19574
                     /locus_tag="PK754_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNB08126.1"
                     /translation="MSILHDNFLGPKAENAEALQESLIKILDWHAAWRRTFFTNDPEV
                     FDRSATPSDNTLQKHLDVLLERLSHQPPYFSPRYAAQMLKDPALSSTLGYLAAILTNP
                     NNHAYEGGPITTELEMECVQDLLNLCGFKKGWGHLCSGGSLANTEAMWAVRDVYTQKH
                     KKPGAVLFSHMAHYSWKRICSILAIQDGVEIPTDSKFRMDLDALESVLKKKKTMMVVA
                     NIGTTGVGAVDDIEGILQLRKRYGFHLHLDAAYGGYSRSIIIDASGRIKPYANVALIV
                     KPEVYLSLRAMKDADSITIDPHKQGALGYGCGAVIYKSEALRSAILNTAPYTYHIKDK
                     PNIGMFTLEGSRPGAVAAGCWLTHRMMPLHEEGYGRLLTENFLAAQELHRRIESESGF
                     RPLHDPDLDIYSFYKPIAASKTASLQKQNKLNTSIYEAFSVLNPKAPFILSKFVVDAP
                     SVKHATGALKTDDRFFLSLRTVLIKHWMRMSKPSYVDLLIEALREKRI"
     gene            20300..21670
                     /locus_tag="PK754_01080"
     CDS             20300..21670
                     /locus_tag="PK754_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08127.1"
                     /translation="MKLKFLATALFGFYLAAITINSCSDNEQPVHANDENAAFPQINI
                     SSRKELFETRGLAKRVANQGIKELSRFFAMDKKWLKKSVLQDTKKAGVPIDQLAVEAA
                     TSQITLDGVDYTIKVELLNSKQAFALDKKDKKFSYGVALDPDLFHADESDIPVYFWNE
                     DSKSIEEKTISLPMSSDTEERGEAMKAAKDAIEYPVFLISLEQTGDDPIIDAEFRALL
                     QKRIKEYADRVVKSNSSQLALLPPPPCEDCPVPPPPPPPPVPVTVPYFVVKQINLFDK
                     KDYTNDEFEMYVGESTENAGEIRGHTIHKFNGNTRSDAAGRSVYYRDVNGDQGWEIMS
                     NDIAIWPLQWIDPNGWMRVVDIENDDDAGKHDNVNDGSTISVDIKHYDVFAQSVKTNL
                     WKFYVAGGTLFSTDFDDIYGQSGIDKINVATVNAKLGSGYLNDYIKLEGRILNDIDYW
                     VGVRKY"
     gene            21741..22664
                     /locus_tag="PK754_01085"
     CDS             21741..22664
                     /locus_tag="PK754_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08128.1"
                     /translation="MKQTIALVALFFAVSCSCNDAPEGSPYLEKDYNYITGILVPDSF
                     RTYRQEIFVGRMLDEKVGSGEVDINMYGEDTTLWLQNLVTWHDYRGKLVGTEGATVKI
                     KDQDNHEVVFQDIGKGFYRDVENALKIKALNHYELTVVTKGRTFTAETSVPGDFYVTN
                     LIEGDTIIETAQREEYFYWAEHKPSWTISSGTFYYRTDHHASAFEFNVINHVYSPPGF
                     FLVNADTLNHKLPLLYSGWFQVMAMDSNYGRMYSPEESSTAPIPLLTYLSTQETKPLP
                     SRTNIKGRDATGVFGSINRTKRISFYFKMVK"
     gene            22664..23593
                     /locus_tag="PK754_01090"
     CDS             22664..23593
                     /locus_tag="PK754_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08129.1"
                     /translation="MKYHVVVFLFFLAISCSCGDPEREDSPYLERDYVYITGILVPDS
                     FRAYRQEVFVGKMLDIKIGSEEVDTTHYGEDPLLYIQNLVTWHDYRGKLVGTSGATVF
                     IRDQYGTEVQLLDIGNGFYQDVANELKIKSMITYTLIVTIGNDTSEATTTVPGNFQLS
                     NVTDGDTVDVTVRPYRNTYGGDFFPYWSLSNSTFFYRTLRSTSLFPPIILKHIYSPPS
                     VIPLIIDTTEVSIDTVVASWIEVTSLDSNYGKMYSPEQTSTGPTDLLTYLSDQETTSL
                     SKRTNIKGRNVAGVFGSLNRTKRVNFYMKAVKQ"
     gene            23628..24557
                     /locus_tag="PK754_01095"
     CDS             23628..24557
                     /locus_tag="PK754_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08130.1"
                     /translation="MRIKHTFIVIIMFSVLCSCNDAPEGSPYLDKDYNYIVGILVPDS
                     FRTYRQEIFVGKMLDILVHSNEVDTAQYGEDPVLHIQNLVTWHDYRGKIVGTTGASVE
                     VIDITDNESVTFQDIGNGIYRDVNEQLHVHPLHRYSLKVTTTEKAFTAETTVPGDFQI
                     SNINEDDTLTVVAIKRSYYYSANYPPYWTSSQGKFFYRTDHLTSQFFINFINHVYEPP
                     GFFTVTPDTVNYQLPFIFSGWIQVMALDTNYGRMYSPEETSTAPIDLLTYISNQETTP
                     LPERTNIKGKEAVGVFGSLNRTKRVNFYMKVVK"
     gene            complement(24715..25995)
                     /locus_tag="PK754_01100"
     CDS             complement(24715..25995)
                     /locus_tag="PK754_01100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB08131.1"
                     /translation="MRSLFKKIINHYRVPDDAYETFRHDFKYGISKGLVLAGSAGVAG
                     VILKKSFSASDFQVALLYSATNLGLLFSIFTAVHAERANKMNYAYVPDAIARTLFIFT
                     AVATALIPNAWLFTAILCAAYAFNSLNTPVLTSIYRSNYPDHSRGKVMGIVRTVFNIA
                     FVVASYVFGLMLDLDGHNYVYVYPIIGLVGLWGGWQLRKIKIVPTVHTTPPMHPIRSA
                     LTILRQDKPFAHFMGYWAIFGFAMLMIDPVKTIYVTDPAYGINATYEEAVLAVTVIPQ
                     AVMLLTFAFWGRQIDKYGVIKIRFLLNILPTINVILFYFATDLRLIYFASFLQGISMS
                     GAQLSWQLCVMEFAPRQQVGLYMGIHTMLTGLRGIVAPFIGAWLIGTSGMGNTFLFAA
                     GLMVISTVMMIRFSANHIRRDIEVPLPIAAERGD"
     gene            complement(26037..26618)
                     /gene="pdxT"
                     /locus_tag="PK754_01105"
     CDS             complement(26037..26618)
                     /gene="pdxT"
                     /locus_tag="PK754_01105"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HNB08132.1"
                     /translation="MQKKIGVLALQGDFEAHQKMTQSVGADAVQVRKPSELEGLHGLI
                     IPGGESTTLIKLMRAFAMDDAIKAFYDKGGAIFGTCAGSILVAKTIVNSPQFRFGLID
                     ITVERNGYGRQVDSFEKDITIPVLGEKSVHAVFIRAPRIVAAGPATEILSSVGENILA
                     ARSGRVLVCTFHPEITDDARMHRYFVEKVVGTK"
     gene            complement(26635..27150)
                     /locus_tag="PK754_01110"
     CDS             complement(26635..27150)
                     /locus_tag="PK754_01110"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB08133.1"
                     /translation="MKDKIEKLRRVHRVARAGLIKTLDGITEEQLYWQPAAESRSIGD
                     MMRHLIRVDMWFYKRHDIAPDVADIKRPTVTQLQTMLQQALGQYEAILDGLESDALLD
                     RRTTATDKVPYKSLGAVMAHIPQHYLYHMSQMIYLRRAQDRTWESPLPEWETATDVIA
                     QYMVGMAGEHE"
     gene            complement(27170..28051)
                     /gene="pdxS"
                     /locus_tag="PK754_01115"
     CDS             complement(27170..28051)
                     /gene="pdxS"
                     /locus_tag="PK754_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HNB08134.1"
                     /translation="MLKGTFKVKVGLAEMMKGGVIMDVVNPEQAKIAEDAGACAVMAL
                     ERVPADIRRDGGVARMSDPEMILGIMDAVSIPVMAKARIGHFVEAQILESLGVDFIDE
                     SEVLTPADEANHIYKHDYKVPFVCGATNLGEALRRIGEGAAMIRTKGEAGTGNVVEAV
                     RHIRTIIGHIKKLTVMGTDELMSEAKTMGAPFELLQWVAKNGKLPVPNFSAGGIATPA
                     DAALVMQLGAEAVFVGSGIFKSGNPVKRAEAIVKAATHFKDPAILAEVSKSLGAAMVG
                     INVDNLAASELLANRGW"
     gene            complement(28137..29057)
                     /locus_tag="PK754_01120"
     CDS             complement(28137..29057)
                     /locus_tag="PK754_01120"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNB08135.1"
                     /translation="MSSHKHYWPHIAIAICTLIWGSTWLAIKIGLEDLPPFLFAGIRF
                     VIASVLLLALMFFQRIRFPRDRHSWHVMLFLGFFQMLDYAFVFWGEQYIDSGIGAIIF
                     ATMPFFVIILGYLWLAEHKITGVKIFGTVVSFAGVTVVFLRDIHHIGTSWMGDVSIIL
                     AALCGAVISVYAKKHADGIHPVVNVTVQMVICAVCLLAAGLLTEDTSRLHWTPQSVAS
                     IFYLGVFGSAVAFVVYMWVIKKVSVVEAAIIPVATPLVAVILGWLVRGEALTVRAMIG
                     CVLILIGVYFVNVYRSRARTAVQTIVEDHN"
     gene            complement(29094..29585)
                     /locus_tag="PK754_01125"
     CDS             complement(29094..29585)
                     /locus_tag="PK754_01125"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNB08136.1"
                     /translation="MQGIIKQINISSGGIPKKPTASAYVSSERVQGDDWNNKNVHGGP
                     RQVVLMIANETYQSLVQLGYPVFPGALGENFTTEGLAYAPIRFGDTFLVGGKLILRIT
                     KLRVPCNTIKVYGADIGTHLYDALARDGNPESPHWGTGGFYAEVLQEGTVQAGDVIRI
                     KKD"
     gene            complement(29591..30052)
                     /locus_tag="PK754_01130"
     CDS             complement(29591..30052)
                     /locus_tag="PK754_01130"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB08137.1"
                     /translation="MMNTKDFIKRWQYEIWADRLVSDSIMALPAGRGRASQLFSHLIE
                     SKVLWYQRIHGEHKHITTVWSDLPITRCLDVLSEINSNWDKFVHELSEEVLQRTITYK
                     TMNGQPYQSTVHDILTHILNHSTYHRGQIAMEVRKAGGTPAQTDYITFCRE"
     gene            complement(30060..>31101)
                     /locus_tag="PK754_01135"
     CDS             complement(30060..>31101)
                     /locus_tag="PK754_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNB08138.1"
                     /translation="EMGDTQGFRPFVEYLPKFLVRRGLTVQSSDITMVSGIQQGIDLI
                     ARILINPGDTVITEEVTYHGARRIFKSLGANIIGIPMDDQGMRMDILETILARQKVKM
                     IYTIPTFQNPTGRTMPLDRRKHLLELAYHYEIPIVEDQYANELRMEGEEIVPIAALDD
                     EGIVIALGSFSKILFHGIRLGWVITSNRAFQNKLNYAKRLTDWQNNYLIQGAILEFCE
                     AGHFDRYLKRKMKVVKERRDAMITASRQYFPEEVRFHVPAGGLFEWADLPDGMDEYDV
                     LMEARQRRVLFTPKRFFSVRPDAGGGMRLGFVSQTTEHIHEGIAILGDIIKKKIGSAP
                     WVESDSALASVM"
BASE COUNT         8707 a         7256 c         6698 g         8440 t
ORIGIN      
        1 ccgcgtttgt ccaacttacc gttttgatac tgaaaaaact cctcgttaac attgagatgg
       61 ccgtacatat tgagaatatc cacgaagctt accacggcaa aacgtccgat ttgcttccga
      121 taatccacac gaacattcca tgtgtgaaaa tcatccaaac gatccccgtt gttacgaatg
      181 atctctttgc tgtaacgcat atgatccgta tcattaaata cattttcgtg aatcacatac
      241 gtgtccttcg ggcgacccgt cgcgtatttc catttcgtcg aaaatgacca ttctttgtta
      301 aattcgtacc cgaataaaac attgaaaatg ttgggctgag aaaaatccga attgtaccat
      361 ccttcaccgt tgtggtcatt gcgacggctt gccatacgtg agtagttgat ctggccgtac
      421 catcgttgtg caaaacgttt gacaagaccg atgtccaccc cgcgtgccca accttcgccc
      481 tgattggtac gcagattgga tgtacgatcc ggctttacga taagatgatc aagtttttta
      541 taataccctt cgatcgtaag tttgacatca ttaccgagat aacgtgtacc accggcaatg
      601 agatgcaatg ctttctgatt tttaagactc ttatttcgcg catcactacc cgtaacatcc
      661 agctctggcg cttgataata cagtcccgta gcgccattaa gcgtcgttcg cgcattcaaa
      721 acataactga tgctcacgcg cggcgaaatg gcgtgccgtc tattgaacgt actgtattca
      781 taccgcaccc ccggattgag tgtaattttt ttgtccggcg aatagcttaa ttctaaaaat
      841 cctgcactct gatagcgtgt ctgtttgttc gcataactcg tccttgccgg atcacgcaca
      901 agatacaatt gggatatatc tgaacggtaa tcatctttat cgtacacaaa ggttgtatcc
      961 caaccgtttt gtttgagcgt attatccatg ctaaagcggt tgatttctaa accggtcata
     1021 gctgtaagat gagtgaaaac ctggtatgta aattgcgatt tagcacccaa ctgttgttcg
     1081 ttgaaatcat agacataaat attatccctc gttctcactt cggactgtgc aggggcgatt
     1141 ccgtttgtcg cgtcggtata ggcgactccg cttttaaacg ggttctccct gtgaccgtaa
     1201 taaacgaccg attccataaa acttttttta ccggtaagcc agcgccagtt gagcccgatc
     1261 agatattttg tttcaccgcc ttcagctagg tcattgccat acagattatt gctttcgtat
     1321 acgtgcgaca gatttcgatc aaataaatca tccgcataaa tgccgagaaa ggacaactta
     1381 tgtgccgatg cgatgtctgt cgtcgttttt acgataatat cggtatagcg cggatggccg
     1441 agatcttttt gaccggtcca tttcagaata ttattaaaat cctgatgacg tgcgctcaga
     1501 agaagactcg tttttttatg aatatacgac ggtccgtcat aattgatttc ccagccgatc
     1561 gcgtcatagg taccattgac cgtcggcgtt tcacgatttc cttctttgat atgcagatca
     1621 acgagtgaag catttttacc gccataacgc gcaccgaacc cgccggcctg aaaattagcc
     1681 gactcgatca aaccgggagt gaagattgaa aaacgtccac cctgcgcttc ctgctcttcg
     1741 gtgcccccgt caaaatgaga gactttacta aacggaatgt tatccacgag aacaatatta
     1801 tcccttgggc ttccgccacg aacagagaaa cttgaaaact caccaccgga cgaactcaca
     1861 ccgggtaagg tttcgatcgc acgaaaaatg tctccggtag ctccgggact gcgccgaatc
     1921 tcttcgcgtg aataattata attagatacc ggcgccgatt tatcttgctc aaaacccgaa
     1981 gcctcgacgg ttatttcttc cccggcaatg gatgtctcct gcatttcaat ctcacgcaca
     2041 taagtgattt ttccccgaac gacacgaata tcggtttcaa aatgcgattg gtaaccgatg
     2101 tagtccaatt gcaatttata cacgttttca cttaatccgg aaatagtata aacgccattg
     2161 atatcgctaa tggctcccgt tgtgctgccc acgatacgca ctgatacgcc cgctaaaggt
     2221 tgtttaccga cagcatctaa cactttacct tttatggtac ctttttcttg cgcgcccaaa
     2281 tgcgcaatac acaatacgct caaaatgaga ataacacgtt tcataatagc cctttctgaa
     2341 tgatgtttag tttttatgtg taattatcta ttaaaaaatg atttgtctat taatttttta
     2401 taaacgctgt ttagttatga ggtaaaaatt tttattttgt ttgtttactt gttttaatga
     2461 gagcttgcaa acattcatgc gctccattaa aaataatcgg cttcatttct tcgggagcta
     2521 cgagcattcc acgcagaatc aaagtggcca taccgtgtac ggcggcccat gtcgtaaacg
     2581 aagcgatctc tacatcaaaa cctttaaaat agccttgatc aatacacttt ttaatcgtcg
     2641 cacgaagcag attgtaagac atttcactaa agtcggttcg tatcggttca ccggtgtttt
     2701 catttaggca atgttccata aatttgcgcg gctcccgcat cacaaacatg agatcgtaat
     2761 attcgggaaa atccatcgca aaacgtatgt acgcttgacc catgctgtac gattcatctg
     2821 tcggatcatt gaaactttgt tgggcggttt ccagttgacg tctatataaa atttggaaac
     2881 cttcgttgaa gagttcgtaa taaatttgat ctttgttgtc gaaataaaga taaatagccg
     2941 ccgggctata ccctatttct cgcgcaatat cacgcatagt aacattcgca aaaccttgct
     3001 tcagaaataa ggctttggaa tgcctaagca ccaatgcttt tatatcttgt ctttcgcttt
     3061 cttttggtct tgccatttta atattaatta aacatcgttt actaaacact gttaaaataa
     3121 catgagaacg atccattgtc aaggtaattt ttttgaaaag agcctaaaac tcaattatta
     3181 gattaatact ttcaactaga ttaatacttt caaccatttt gttgggggat atcaaggtta
     3241 aataaaaatc aaaactaaca taaatgcgtt gacacctgat cggttaaatg atttttttgg
     3301 aactgataga gccaagaaat tcttgctttt tctttaaccc atcttcgttg actttcagag
     3361 gtgttttggt tatgttggcc gacggaaagg aacccatgtc aaccacatca aaacgcataa
     3421 cgctgccggt actcggcgaa gcttcaaggc aacgcgcacc acgttccaat aaacaaaaaa
     3481 aagtgaatat cgtttcactt ggatgcccaa aaaatcaagt ggactccgaa gtcattttgg
     3541 gcaacctgaa gcatacgcgt atcgtagata aagccgagga agccgatacg atcgtgatca
     3601 atacctgcgg tttcatcgaa agcgccaaac aagaatctat tcaaactata ctcgaagcga
     3661 tcgaactcaa aaaacgtgcc gctaaaaaag gaattaccaa agaaatcatt gttgccgggt
     3721 gtttatcaga gcgctatccg aaagaattag ccgctgaaat gcctgaagtt gatgcttttt
     3781 tcggcgtaca ccagtttgac aaaatcaccg agcgtatcga agggcattat cagcaagttt
     3841 tgtttaccga acgtattctt ctcaaccagc gtcactacgc ctatctcaaa atatccgaag
     3901 gttgtaacca gcgttgcggg ttttgttata tcccgatgat tcgcggtaat ctcgacagca
     3961 aatccatcgc cgacaatgtc gctgaagccg aaaaattagc ctcgctcggc gttaaagaac
     4021 tgatctgcgt ttcccaagat acttcgtcgt acggatatga ttttgatcgc agcgccccca
     4081 atctccggca gaacaaacac ctgatcgaac tgttgcatgc tttgggcgaa gtgcgcggca
     4141 tcgaatggat acgcgttatg tatttgtatc cgtctctatt ttccgatacc ttgatcgaaa
     4201 ccattgcaac acatcccaaa atctgcaaat atattgatat gcccgttcag catatttccg
     4261 atcgcatact aaaaacaatg cgtcgtaata ccggtaaaaa gcaaaccacc gatctgctat
     4321 acaaaatcaa agcgcgcatg cccgatgtcg ccctacgtac atctatgatc gtcggttatc
     4381 ccggagaaac cgaagcagac tttgaagaat tgtgtgattt cgtccgcgaa tttaagttcg
     4441 atcggcttgg cgtatttatt tattcgcaag aggaaggcac ctacgcgtat gatttggaac
     4501 ctcaagtcga agaagccgta aaaaaagaac gctttaaccg tttgatggaa atacagcgtg
     4561 aaatttctca cgcccataac atggaaaaaa ttgatcaaac atttcgtgtg atgattgata
     4621 cccaaagtag cgagcactat attgggcgga ctatgtcgga cgctccggaa atagacaatg
     4681 aagtaattat cacttcatcc cggaaattaa aaccgggaca attcgttgat gttaaaatta
     4741 ccgatgccac ggaatacgac ctgtatgctg aagcgttata aagattagtg ttaactttta
     4801 aacgatatgt attgtgggac atatatcata acctcatcta agctcaagga gtggtcaatt
     4861 tgaaacgaat tttaagcctt atcgtgctca cctttttagg tagctgtgcg ttgcggggac
     4921 aagcgaacat gggcaaaacc ggtggcggcc cttttttctt tttggatgca gccaatgtag
     4981 ccgccaaaga caccaccaaa ggacgggtgg aaattttcat caaaatcgca tacagcgaat
     5041 tgttatttgc ccgatacaac caggtactct tccgttcgca atacgaagtc aattatacgt
     5101 tagccgacaa aaaaggcaac gtcgtgcttc gtgagataca agataaagag attgttacag
     5161 agcactacga agaaactctt tccgatctgc ggtttcactt tagtcgcctg acgctcaata
     5221 ttgatccggg tgaatatatc cttaccgttg tcattacgga taaagagaca caaaaattcg
     5281 gtcagcgtaa actgagcctt ccggtgcgta catttcgtga aaaatcaatc ggtataagcg
     5341 atctgctttt tgccgataaa attcaaaaag acagtgtgga cgatatcata cacattctcc
     5401 caaatgtctt taaaagtttt gataatgagt ttaaaaaata tgccgtgtat tttgaaatct
     5461 acaattccaa atacgcctgg aaaaaccgcg actccatcgg aaatctcccg gaggaaaaag
     5521 aacctgtacg tattcgctat cgcgtatatg ataaaaatca aaagatcgta acggaagatt
     5581 ccaccgtccg tgaggtcaat caatttcaaa cgttcagttc gatcgagatt gataaaagta
     5641 aagtgagctt cggcaaatat gttttagatg tgatggtgta cggtaaaaac ggtgtccgcg
     5701 ctacctccaa agcaatattt gacgtgcggc tgtctacgtt taccgcaccg tcgttatcgg
     5761 caacggcctt tgatttggat gtcgccatca aacaaatgcg tcatgtcggt cgaaatctga
     5821 acacagataa aattctcaaa gggacgcaaa aagaaaaaag tgattttttt gaaaacttct
     5881 ggcgcgctaa agatccaacc cccggcacgg aacgcaacga aatcatggaa gaatactttc
     5941 gtcgagtgga ttatgccaac cgttatttca catccggata tcgggaagga tgggatacgg
     6001 atcgcggcat ggtttttatt atattggaag ctcccgatga tatcgaacgg cacccttttg
     6061 ataacaatga caaaccctat gaaatatggt attattacca agccaatttg aggttattat
     6121 tcgtggatgt caatcagatc ggagactacg aattaatgaa tcgtcaagaa tttgaaagtt
     6181 acctctacct gaggcgttga tatcatgaat gaaacgaaca acctaggcga gccaacatcc
     6241 gctatgatac gctgcggata tgtagcgatc atcggccgtc ccaatgtcgg caaatccact
     6301 ctgatgaatc agcttttgca tgtcaaacta agcattgtca cgcccaaacc tcaaacgact
     6361 cgccagcgcg ttctcggtat actcaatgaa cccggtacac aggccatttt tttggacacc
     6421 cccggactca ttgcgccgca atacgcttta caaacggcca tgatgaaaat agcggaccaa
     6481 tctatggaag cggccgatgt gctgcttatc atgattgacg gaacttcaaa agccgagtcc
     6541 gattttgtac aagaactgtt taaaaccaaa cttctgcact ccgatatccc caaagttttg
     6601 gcgatcaaca aagtggatcg aatggccaaa ccggaattgc ttccgatgat ggatacgtat
     6661 atcaagaccg gatttttcaa agaggtcatt ccaatatcgg cgctcgaaaa tgacggtgtg
     6721 gaacagatga aacaagcgct tttcaaatac ctgccgttga acccgccatt ttatccgccc
     6781 gatgcgttga ccgaacaacc cgagcgtttt tttgtagcgg agataattcg tgaaaaaatt
     6841 tttctccatt acacacaaga agttccttat tcgacggaag tgatcatcga agaatttaaa
     6901 gaaagagaac gcggcaaaga ccttattcga gccataatca tagttgaacg tgattcacaa
     6961 aaagcaattt taataggaaa aaaaggcgaa gccctcaaac gtatcggtga aacggcacgc
     7021 aaagatatcg aagcctttct cgatcgtcag gtttttttag aattgtgggt gaaggtcaaa
     7081 gaagagtggc gcagtaacga tgctatgctc cggagtttgg gataccggtg atacgatttt
     7141 tttggattat ggtaatcgca gtttttccgc tgattcaaac ctgtacccaa cctaacgaag
     7201 attcaccgtc ccgtcgagtt cgccaattta cgcgctccta ttttcatcga aaatgggatg
     7261 ccgcccttgt tcattgtacc gatgccgctc gctctaaaat acttaacgag tcctcttttg
     7321 aacgacaact tcccgatata tggaaagaac ctgagatttc gatatcggag ttttatgtca
     7381 aagattccat accggaccct accggtacta tgatttatga cttagtactg ctcgatcggg
     7441 atgctaccgg tggttttcag gaaagtttgt ttcggataac tctgaaaagc gacagcggcg
     7501 ggaaaatcag taattatcaa tttatacgga gtgactatga tatccgttga tgaagcgctg
     7561 cacatcatat tatccaaagc taaaccgctt gcgatcagaa cggtgccatt taaggatgcc
     7621 tgcggttatg ccctcgcaga aaatatcgtg tcccgcgaaa tgataccacc ttgggataac
     7681 agtggtgttg acggttacgc tgtgattgct tccgatacaa gcaaaaccaa acaaccgtgt
     7741 atgctaaagg ttttggacac agtccatgcc ggcgatgtat caacaaaaaa attgacagcc
     7801 ggaacagcta ttcaaatcat gaccggcgcg cctgtaccgc gaggaacgac agctatcgtc
     7861 atggtagagc atacgcgtcg cgtcggccaa tgggttcata tacaaaagcc ggcccgcgaa
     7921 ggtgaccaca ttcgtaaagc cggatccgat atccagaaaa accagatcgt tttttcacgt
     7981 gatcacatca tacgacctta tgatcttggt gttttagcgt ctatcggaaa atccaaaatt
     8041 cgcattcaaa ccaaaccgac catcgcattg cttgcgaccg gtgacgaatt gttatccgtt
     8101 gaagaaaaac taaaacccgg caagatacgc gccagcacac attttgcctt gaccgcttta
     8161 ttacaaaaag ccggttaccc ggtgatcaat ctgggaattg cgcacgatac acccaaagcc
     8221 atcgccgctc aaatacaaaa agcattaaaa gcagacgtca tcatcacaac gggcggcgta
     8281 tccatgggcg aaaaagatta tgtacgccaa gcagtagaaa aagctgggat aaaaattcac
     8341 ttttggaaag tccggcaaaa accgggaaaa cctatggttt tcggatcaaa aggaaaaaaa
     8401 ttgtttttcg gattaccggg aaaccccgtg agttcgcttg tatgcttcga actgtatgtg
     8461 acgccggcaa tagcgcgcat ggcaagtcaa ccgtcgccga ttttgcgcat tccggtccgt
     8521 ttggataacc ctgtttccaa aaaacccggg cttcggcatt ttttgcgcgc gcgcctttat
     8581 atccatgatg gtcagatcaa ggcttcttta tccggcgaac aaagttccgg tgttctctcg
     8641 catatggctt atgcacaagc gctcttagat atacctgaaa acgacggaga taaaaacaaa
     8701 aatgacaccg tagatgccgt cgtattggat cgcgatcatc tcaatcaatt aatcatttcc
     8761 attctttgag cgatgacgac acattttgat atacagccgc tggagtttga taaactcaaa
     8821 gagttgattc gccaatgcgt gctcacacct atggcggacg cctgtgtgca ggcgatgaaa
     8881 cccatgacgg atgcggatct gatcgaaaaa cgtttggata tgttggtaga aatggccgac
     8941 ctattgcgtt atgatgatac atttccgatt gataccttga ccgatatacg tcagctttta
     9001 gatcgcattc attcgcacgg aacttttcta ccttcatctg atttattgct tgtagcgcga
     9061 tttgtcgaaa cgacacgcaa aataaaaaac tacgtacgtc atcgtcatga aaaataccct
     9121 ttgctttcgc gtgacacgga tacgttgcac gatctgcgaa ccattgaaga tgctattcaa
     9181 catgcaatcg acgatcacgg caacatcaaa gatcacgcca gcccgaagtt acgccaattg
     9241 cgtcgcgata ttgacaataa atcgcacagt cttcgaaaga agctggaatc tcttgcccgg
     9301 caatttaccg atgccggtta ttcacaggag gctatcgtaa ctatgcgcga cggacgcatg
     9361 gtcataccgg ttaaggatga atataagaat accgttcgcg gttttgtcca cgacacatcg
     9421 gccagcggtc aaacagtttt catcgaaccg gccgaggggc tcgaactcaa caatgaaatg
     9481 cgccgtttgc tcatcgacga acaaagagaa gtcgaacgca ttttgatcga aatcgccgat
     9541 acgattcgcg cagaccgtcc cgcactgtat gaaaacatcg acattgcaac catgatcgaa
     9601 tttttgttcg ccaaagcacg gttttgcaat acgcttcaag gtatcaaacc caaactcaat
     9661 accagcggat acattcaaat taaaaaagga tttcatccgc tcctgctgca taaagaaaac
     9721 cagaaaatgc cggatcaaag gcgtcccgtg ataccgctgg atatagagat cggaaaaaat
     9781 tttcgtacac tcattatttc cggccccaat gccggtggaa aaaccgttgc cctcaaaacc
     9841 gtcggacttt tcgtattgat gacacaatgc ggtttgttga ttccgtgcgg atttgacagc
     9901 gaactgagtc tttttaccag actttttgct gatatcggcg acgatcagtc catcgaaaat
     9961 gacctctcca ctttctcctc gcatatgcag cacctgtccg aaatggcgtc tcaactggat
    10021 gatcaaacgc tgattcttat tgacgaaatg ggaagcggta ccgaccccaa agaaggcgct
    10081 tcgctggcta tagcgctgct tgaatatttt aacaatcacc gcgccgtctc tatcgttacg
    10141 acgcatcacg gcgaattgaa agcgtttgct cataatacgc cgggtgttga aaacggctcc
    10201 atggaatttg atcaagctac gttagaaccg acctacgttt tcagaccggg tattcccggc
    10261 agcagttatg cgatggaaat ttccaggcgt atcggtttac atcccgatat catcgagcgc
    10321 gctaaaagtt tatccggtac cgaggcatta cggctcgaaa cactgataca tgatcttcaa
    10381 tcccgcgtaa aaaaatacga aaccgacctg gccgatatca atcgcgagaa aaccgggctt
    10441 gaaggcatgg ttaagtttta caatgatcgg tccaaagaac taaagcagaa ggagcgcgca
    10501 cttaaacaga ccattttaga agagaagcaa cgcgtactgg ccgaagctaa taaaaaaatc
    10561 gaagcgacga ttttggaaat caaaaaagcc gaggcttcca aagaagcgat caaatcggcc
    10621 aaaacctcca tacaaaacga acggctcgcc acggatcaag ccttagctga tgaaaaaaaa
    10681 ttaaccgaat tcgtatccga accgcttcat gtcaaaaaag gcgatcgtgt ttatatacga
    10741 catatggata tcgaaggaac ggtcatcgaa tctcccgaaa acggtggtca ggcgcttatc
    10801 gccgttggag ccatccgcct taaaattgat cctcgccaac tttccccggc tcaaaacgcc
    10861 ggtgagaaaa actcttctcc cgcacgtcag accatccaat gggataccga cgatatcggg
    10921 caagaacttg atttacgagg tttgaccgcc gaagaagcgc tatatgaggt agataaatat
    10981 cttaccgatg cggggatact agggttcaga gaagtgacat tactccacgg caaaggctcc
    11041 ggcatactta gaaatcgtat aaatgaattt ttaaaacgag atcgccgaat tgattcctat
    11101 cggctagggc agtatggcga aggggatacc ggtgtcaccg tggttgtttt gaaaaacgat
    11161 tgatccgctt tagtcatccc ataagctttt gaaatattct tcataaaaat ccatcgtctt
    11221 atcatagatc acatcttgcg tttcggctat ttcaattacg gtcatttggt cgtcatgtat
    11281 cggcgtagcg gattgaattt gagcgaaaaa gtttttcacc gccggttcgc gttcaacttc
    11341 cgaatcaaac tcgaacatca aatcgtatag aagcgacaaa taataggcct cctcgatttc
    11401 ttcataaaaa tagcgttcga tcagttttct taatgtacgt ttaaaaactc ttccgcgatc
    11461 tttttctgag gtgacggcat cgccgatgat catatcgcgc aattcatata tgtaatcccg
    11521 cgtcaactcc gaatcttttt taccggaaat atccacgaga taccgttcga tataattttt
    11581 tcgaccgcgt ttgtgcgccg atatttgtaa aagtggtaag gccgccgaac gcagttcacg
    11641 cgccgaaatg acaccgcgaa gaaactggtc aagaataccg accatctgac gtttgaggga
    11701 tggctttgtt gtcatcatag ttaatacgaa aaaaccttca ggattgactg aaggtttttc
    11761 cgaaaacact taaatatggg ttaatgagcc ggcctccaaa tcggataata ggcccgactg
    11821 tctttggata tcattttttt ctcctccgtg ccatcgcggt taataagata aatataaccg
    11881 cgactggatg tacgttttga atatacaatc cggttaccgt ccggagtcca tgccggatca
    11941 atattgttcc ctgattgggt aactttttgt atacccgtac cattcggatt cattgtgaaa
    12001 atattcagac tgtcgttgcc attttttgaa gaggagctgt agaatgcaat tttatcgccg
    12061 gatggtgaaa accgtggtct accggcaata gaggcattca aatcggtaag ctgagttttt
    12121 cccgttccat cggaattcat aatccaaatt tcttcgttcc ccgttttaga cgatgtataa
    12181 actattttag atccgtcggg tgaccatgaa ggatggcaga acatatctaa tttatctgtt
    12241 tcatccgtaa gacgaacagc tgtcgtgtca ccgtagagcg tatctgtaat cgtagtcgta
    12301 taaatgtcaa aagttgaatc tcgattggaa ataaatacca ttttattggt catcagagac
    12361 caatccgggc gcatatttaa gacaatacct tggaatgtaa aattcgtatc aaggtaatat
    12421 ttgctataag tgacaggagt aagtgtcgaa ctatccatat cacggatata tatattggag
    12481 tgtagcggtt gacccgtaga ttgattaaat aaatgaatat tagtataagc aaatttgcga
    12541 ccatcggctg accacgttgg atccaattcg gaatcattaa cactaatatc caatatgtcg
    12601 cctcgtgccg tatcatgtat tgcgttaata tgttgcgaac caaatatttc tgaaacgtat
    12661 aagatatcgg gatacacgtc cgtcaaattt agaactaacc tcggactgta tgggttatta
    12721 tcgtcgcatc cccacaaaac cattaaacat ccgatcaaca caagaacgat agattttttc
    12781 atatttattc cttttaatct tttatggttt caaacgatga atcatgcgcg ggaaaggtat
    12841 cgtttcacgc agatgctgaa tgccacaaat ccatgtaacc gtgcgctcaa ccccaagtcc
    12901 aaaaccggca tgaggaaccg ttccatattt acgaagatcc aaataccaat caaaagcctc
    12961 gaccggcagc ttatgttcct gaatgcgcga caatagcgct tcataatcat cttcacgttg
    13021 gctgccaccg acgatttcgc catatccttc cggcgccaga atatccatag ctaaggcttt
    13081 atccggttca ttgggatcac gcttcatgta aaatgctttt acggccgatg gatagtgatg
    13141 aacgatcacg gggcgatcat attttgccga aatgatcgct tcgtccggcg caccaaaatc
    13201 attaccgtat tcaaaaggtt ggttcgcttc tttcagtatt tttaccgcct catcataatg
    13261 tacccgagga aacggcgcct ttacacattc taatttggtg atatcgcgtt cgagagtttt
    13321 taattcctcc atacgatctt ttaatacagt ttgtacgata tactctagga aatcctccgc
    13381 caattccatg ttgtcataca gatcattaaa tgcgacttcc ggttccacca tccaaaattc
    13441 ggttaagtgt cggcgtgttt tggacttttc tgcacgaaat gtcggtccga agcaatacac
    13501 ctttccgaat gccatagcgc cggcctcggc ataaagctga ccgctctgcg tcaaataggc
    13561 tttggtatcg aagtaatccg tttcgaacaa agtcgaagta ccttcacatg ctgccggagt
    13621 gaaaatcggc gtatccacga gtgtaaaacc acgaccgtca aaaaaatcac gaatggcttt
    13681 gataatcgta tggcgaatgc gaaggacggc atgctgctgt ttagagcgca accacaaatg
    13741 ccgatgatcc atcagaaaat caattccatg ctctttggga cttatcgggt aatccttggc
    13801 ataacccaat aaaaccacct cggtcacttg caattcgtat ccaccgggtg cacgctcatc
    13861 tttttttact tttcccttaa ctgacaagct tgattcctgt gaagcgctat ccgcagcctc
    13921 ccacgattca ggggtaacgc tcggtttgta cacggtagct tgtatcgtat tggttccgtc
    13981 acgtacaagc aaaaagttta acttgccgct ggaacgtttg ttgtataacc accctttgat
    14041 ggtgacttct tcaccatctg aaaacttacc tttaaaaaca tcttcgatat aaacacgcga
    14101 acgcataaca catactctca tcaggtatta aaaaatgaat taaattattt ttttatcatt
    14161 gtttgccaat tgggtgaagc gatttcgcca cgcgcattcg agtgaattaa ccttaatgta
    14221 ccggattcat ccatatcagc aaatacaaaa atagtctgcg ccggttgatc ttttaattga
    14281 agaaaatacc aaatttgata gggtttcata tcggattcga attcatgttt ttcgatttgc
    14341 gcaggaaaac catagacaac cattacccga cccatatccg ttttccaccc ctgcacattg
    14401 ggcgttgtaa acttcatatt agcttcttca taccgcttgt aataatccat aagtttttcg
    14461 ttggatttag tacccggcga agggtcgcga tctttccaaa aacgatctaa aaacttcttt
    14521 ttaccttcaa attccaattg cgaaaaaatc tttttttcat cttcgcgacc gatgagcgaa
    14581 acgatatttc ctgcacggat ggctccggct tcgtccaaat ctttaaaaaa cgaatcgtcc
    14641 gcttgagtct cataaatgga ttcgccttcg cgatagacca cgaataattt tttgcttata
    14701 gcaacggatt tactggcatt gtccgtgatg cgcaccataa aaacataacg accgctcggc
    14761 agcgaaataa cattcacgtt atgcataagc agggatgttt caccggcttt attgatctgt
    14821 ctggacgggt actctttgat gatgcttccg ttttcgtccg ttacgataaa ttctgccgta
    14881 tacgtatttg tcgtcgcatc atccggcgta tgtttcagat tgtacacttc cgaataaaaa
    14941 gataaccgag gcagattgga gccgtaaaaa cgtgtcggat tgggataaac cgtatatccg
    15001 ttttttacaa acatttcacc gctatttccg cctttgagcg cttgcgtcgc aaactgaatt
    15061 tggctgatca tcaagtcact tttatcaaaa tcatttatct caatggattc ggtatagctt
    15121 ccgctccggg acgcatcgtt tttatcctgt atcgtagctt tgagaatata ttttgacggc
    15181 ttcagcaata aaggatattc aaacaacaaa ggcacttctt tgcccgtgtc cgcctccgca
    15241 atgcgatcat ccattacggt tgtccatgtg ttttgaacaa catttttgct gtcgttgaga
    15301 tcggtaaact ccaaactcac cgtatatgcc gccgagtacg atgtatcttt tttcacaaaa
    15361 ttgaactgat tgcgggacat ataaaaatac aactgcgtgt aggtaacatt tttttgattg
    15421 aacattttaa atgccgcata atccacataa aacgacaacc gccccgaact ctgattgccg
    15481 gaaccttttt gcgcattgat ttgcgtcatg ctcgcccaca gcatcacaca acacacgaag
    15541 agaatacgat gtttcatgcc aactccttac acaatgatcc ggtctgactt agaccggggt
    15601 taatataatt tcgtttaatg cgttaatgcc gaatccatgg ctttcaaaac agccactttg
    15661 ccttctccgg tttcgccgga cgtaaaatca atcggccatt cccatgtgcc gtaggcttca
    15721 ttgagcaatt tgagtatcgg ttttcgctcg ctgtttttca gtttatcggc tttattggcg
    15781 gcgatcaaag tcgggacttg ataataattg aggaattccg ccatctgaag atccgacgcc
    15841 tgcggttcga tacgcgcatc aatgataagt accgccagac ggatacgatc gttttccgtg
    15901 agccacgcgc ctagattacg ttgccaggct tctctaagtt tccgctcgac tttggcgtag
    15961 ccataacccg gcgcatcaat aagataaaac gcatcattta ttttataata attcaggcac
    16021 tgcgtcttac cgggcgtgga actcacgcgt gccatttttt tgccggttaa cgtattgagc
    16081 aaggtagatt tgcccacatt agagcgcccg aaaaatgcta tttccggcaa ctgcggtttg
    16141 ggtacctgtt ccggtttata gacgctggct atgaattcga cggacttaat gatcatgcat
    16201 tcctgtcggg cttcggtagt atacccatct gccctaaaaa aaacaaccgg aatcaacgca
    16261 ttccggttgc tctcagcttc attgtaaaaa aatcaaatat gaacggcttc tttgtacgca
    16321 tccagcgccg cttccatcat cgcttcggac aacgtcggat gggcgtgaat cgtattgcct
    16381 atttctttat atgtggactc caaagacttc gcgatgccgt attcggcgat catctccgtc
    16441 gcttcggaac ctaagatatg tgcgccgagc aattcgccgt acttcgcatc aaaaatcact
    16501 tttacgaaac cgtcacgctc gccgatcgca cgggctttgc cgctggcgga aaaaggaaat
    16561 ttaccgacct tgatatcata ccctttttca cgcgcctttt tctccgtcag accgatgctg
    16621 ccgacttgcg gttggcaata ggtacatccg ggaatggatg aataatcaat cgggtgggta
    16681 tcctttccag ccattttttc gacacagacg atgccttcat gggaagccac gtgagcgagc
    16741 cacggtgcgc cgatgatatc gccgatcgca taaacaccct gtacattggt acggtaatac
    16801 tcgtccactt tgatccaacc tttttcgacg gtaacaccga ttttttcaag gccgatattt
    16861 tccgtattgc cctgcacgcc gatggcgacg aggcattgat cggccgtgat cgtctgtgtt
    16921 ttacctttgc tggaaacggt cacggtaaca ttattggcac cgacatccac tttttcgact
    16981 ttggtatcgg tgtgaatatc catgcctttt ttctggagta ctttgagcag cgtgttggag
    17041 acttcttcat cttcaacggg caaaatcgtc ggcatcattt ccacgacagt gactttcgtg
    17101 ccgaaggtat tgaagaaata ggcaaactcg ataccgatcg cgccggcgcc gatgataacg
    17161 atggacttcg gctgttcggt attggtcatc gcttccgtgc tggtcagaat gcgtttgccg
    17221 tcggctttca gattgggcag catacgcgcg cgggcaccgg tggcaatgat gatatgttta
    17281 cctttgacct gagtggtttc tttgccggtc gcatcggcca cactgagtgt acttttatca
    17341 agaaatttgc cggttccttt gatgacttcg attttatttt ttttcatcaa aaaatcgccg
    17401 cctttggaat tggcatcggc cacatcgcga ctgcgtttga tgatggcttt gtagtcatac
    17461 ttgaggttgt caaaagaata accccattcg gcggcatgtt tcatatgttc atacatctcg
    17521 gcatttttca gcaatgcttt ggtcgggata caaccgatat taacgcagac gccgccgatt
    17581 ttttcacgtt cgacaaccgc tgttttgaaa ccgagttgtg ccgctttgat cgctgccaca
    17641 taaccgccgg ggccgccgcc caataccact acatcaaaat ttttggtctc cattcgatcc
    17701 tcttgattga gataacattt tattttatgt tcataaaagc ctgaaaatta gggcttgaac
    17761 gtaaataaat cgcgtcaata aatcaataat ctatttgtgg agctttacat gccgatctat
    17821 gaatttacct gcaaaaaatg cggtacggat ttttctgaac tttttcattc cagcacgttt
    17881 gacgcttcgg aagtcgagtg tcccaaatgc cgtgcccatg aggccaaccg caagctttcc
    17941 gtttttgccg ccgactgcaa aagctccggc gaaagtatgc ccatgatggg cggcggatgc
    18001 ggcgcgggtt gcggttgtca tatgaattag tttgttttaa tacagttctc ttttttccac
    18061 gtgcttgagg ggttttcatg tccatactgc acgataattt tctcgggccc aaggcggaaa
    18121 atgccgaagc cttgcaagaa tcgctgatca aaattctcga ctggcatgcc gcgtggcggc
    18181 gtacgttttt tacgaacgat ccggaggtgt ttgatcgctc ggcgacgcct tcagacaata
    18241 ccttgcaaaa acatctggat gtacttttgg aacgtctcag tcaccagccg ccgtattttt
    18301 cgccgcgtta tgcggcccag atgctcaaag atccggcgct cagctccact ctgggttatc
    18361 ttgccgccat acttaccaat cccaataacc atgcctacga aggcgggccg atcacgaccg
    18421 aacttgaaat ggaatgcgta caggatttgc tcaatctctg cggtttcaaa aaaggttggg
    18481 gccatctgtg cagcggcggt tcgcttgcca ataccgaagc catgtgggcg gtacgcgacg
    18541 tatatacaca aaaacataaa aaaccaggcg ccgtgctttt ttcacatatg gcgcactatt
    18601 catggaaacg catttgctcc atccttgcca tacaggacgg tgtggaaatt cccaccgatt
    18661 ccaaatttcg catggacctc gatgcgcttg agtctgtact gaaaaaaaag aaaaccatga
    18721 tggtggtcgc caatatcggc acgacaggtg tcggcgcggt ggatgatatc gaaggtattt
    18781 tgcaacttcg aaaacgttac gggtttcacc tgcatcttga cgcggcctac ggcggatatt
    18841 cgcgttcgat tattatagat gcatccggcc gtatcaagcc ctatgcaaat gtcgcgctga
    18901 tcgtcaaacc ggaagtttat ctcagtctgc gggccatgaa agatgccgac tcgatcacga
    18961 tcgatccgca caaacagggt gctttgggat acggatgcgg cgcggtgatc tacaaaagtg
    19021 aagccttgcg atcggcgata ctcaatacag cgccgtacac ttaccacatc aaagacaaac
    19081 ccaatatcgg catgtttacg ctggaaggtt cgcgcccggg cgccgtcgcg gcaggctgct
    19141 ggctcacgca ccgtatgatg cctttgcacg aagaaggcta cggacgactt ttgacggaaa
    19201 attttctggc cgcacaggaa ttgcatcgcc ggattgaatc ggaatcggga tttcgtccgc
    19261 tgcacgatcc ggatttggac atctatagtt tttacaaacc gatcgcggcc tccaaaaccg
    19321 cttcactcca aaaacaaaac aaactgaata cctctatata tgaagcgttt tccgtactca
    19381 atcccaaagc gccctttatt ttatccaaat ttgtcgtgga cgctccgtcg gtaaagcacg
    19441 ccaccggcgc actcaaaacc gatgatcgct tttttctcag tctgcgcacc gtattgatca
    19501 aacactggat gcgcatgagc aaaccgtcgt atgtggattt attgattgaa gctttgaggg
    19561 agaagagaat ttagaattag gacgttcagg aattcaaaag tctggcggga aactttgtga
    19621 ttgagttgcg tagagaatga gggaaacgaa gagatggatt tcacggaatg tttagcataa
    19681 ctgcatctaa acttgcctct tttactgaac taaatttgat attactccta ataactaccc
    19741 ctctcgaaac tggttaattt aaggcttgtt tatacaaaga caagccgcga tacacgaatt
    19801 tatggtaata atttcaatgc tttacggcaa gacttgctga attttatagc gtaacgctca
    19861 cacaaaggtt catttaaaaa agaagtttat gcattttttt atgaatatct gtcttattgg
    19921 tagttgtttt cacgaacttc aggaaatgag ggaagctcta gcttagcaat aattccaatt
    19981 ctatttctat ttccaatggg attagaacaa agacgtatca tataatgtca tttttaaaat
    20041 gactatagct tcgctttaat agctattgta caactaacgt tttggaggga ggtgtgtagt
    20101 cttagtcgtc taaacaggtt tacgcagact tttaattaac tcaaggtatt aacatgagaa
    20161 caaaaaaaag gaagaaccat attacccgaa ttcagcgctt ggagctttga attttaatag
    20221 taataaggtc ttcccgtcgc aaatctatga aatgcgatgt ggatttgcaa cttttgttcc
    20281 tcagaaagag gattaagtaa tgaaactaaa attccttgct accgccctat ttgggtttta
    20341 tttggcggca ataaccatca attcgtgttc agacaatgaa cagcctgtcc atgcgaatga
    20401 tgaaaacgcc gcattccctc agattaatat ttcgtcccgc aaagagctat ttgagacccg
    20461 aggccttgca aaacgagtag ccaatcaagg cataaaagaa ctatcccgtt tttttgcaat
    20521 ggataagaag tggctcaaaa agtcagtttt acaagacacc aaaaaagccg gcgtgcctat
    20581 tgaccaactg gctgttgaag cggcaacgtc ccagataacg ctggacggag ttgattatac
    20641 aatcaaagtt gagcttttaa attcaaaaca ggcttttgcg ttggataaga aggataagaa
    20701 attcagttat ggcgttgcgc ttgatccgga cttatttcat gctgatgaat ccgatatccc
    20761 tgtttatttt tggaatgaag attcaaagtc tatcgaagag aagacgattt ctctccctat
    20821 gtcgtccgat acggaagagc gcggagaagc gatgaaagcg gcaaaagacg ccatcgaata
    20881 tcccgtgttt ttgatttcgc tcgaacaaac cggcgatgat ccgatcattg atgcggagtt
    20941 tcgtgcgttg cttcaaaaga ggattaaaga atatgcggat agggttgtta agtcgaattc
    21001 atctcagctc gcattgctac cgccaccgcc ttgcgaggac tgtcccgtgc ctccgcctcc
    21061 cccgccccca ccggttccgg ttacggttcc gtattttgtg gttaagcaga tcaatctgtt
    21121 tgataagaaa gattacacca atgatgaatt tgaaatgtat gtcggggaat ccaccgaaaa
    21181 tgcaggagaa atccgagggc atacgattca taagtttaac ggaaacaccc gcagcgatgc
    21241 cgcaggacga tcggtctatt accgtgatgt caatggtgat cagggatggg aaatcatgtc
    21301 aaacgatata gctatttggc ctttgcagtg gatagatcct aatgggtgga tgcgtgttgt
    21361 ggatattgaa aatgacgatg acgccggtaa acacgacaat gtcaacgatg gttcgactat
    21421 ttcggtagat atcaaacatt acgatgtatt cgctcagtcg gtcaagacaa atttatggaa
    21481 attttatgtg gccgggggaa cattgttttc taccgacttt gacgatatct acggtcagtc
    21541 gggtatcgat aagattaatg tagccacggt taatgcgaaa ctggggtctg gttatttaaa
    21601 cgactatatt aagttggaag gccggattct taatgatatc gactattggg taggtgtacg
    21661 gaagtattaa tgtgattaat caagacaggg atatgcaaaa tccatatccc tgtctttctt
    21721 atttttgaag ggaaatgtaa atgaaacaaa ccattgcgct agtggctctc ttttttgccg
    21781 tatcttgttc ctgcaatgat gcgcctgaag gttctcctta tctggagaag gattataatt
    21841 atatcaccgg aatacttgta ccggactcct ttcgtactta ccggcaggaa atattcgtag
    21901 gacggatgtt ggatgaaaaa gtgggctcag gcgaagtcga tatcaatatg tatggggagg
    21961 acactacttt gtggcttcag aaccttgtta catggcacga ttacaggggt aagcttgtag
    22021 gaacggaagg agcgactgta aagatcaagg atcaggacaa tcatgaagtg gtatttcagg
    22081 atataggtaa aggtttctat cgtgacgttg aaaatgcatt aaagattaaa gcgttgaatc
    22141 actatgagct aacggttgta acaaaaggta gaacttttac ggctgagaca tctgtcccgg
    22201 gtgattttta cgtgaccaat ctaatagagg gagatacaat tatcgaaact gctcagcgtg
    22261 aggagtattt ctattgggcg gagcacaaac caagctggac catcagttca ggcacatttt
    22321 actatcgtac agatcaccat gccagcgctt tcgaattcaa tgttatcaat catgtttata
    22381 gtcctcctgg tttttttttg gtgaacgcag atacgctcaa tcacaagctg ccattacttt
    22441 acagcggctg gtttcaggtg atggccatgg atagtaatta cggaaggatg tatagtccgg
    22501 aagaaagttc gacggctccg attcctctat tgacttattt atcaactcaa gagactaaac
    22561 ctctaccgtc acgaaccaac attaaaggcc gcgatgccac gggagtcttt ggctcgatca
    22621 atcgcaccaa gaggatttct ttttacttta agatggtgaa gtagtgaaat atcatgttgt
    22681 agtattctta ttttttcttg ctatttcttg ttcgtgcggc gaccccgaaa gagaggattc
    22741 gccgtactta gaacgtgact atgtttatat taccggtatt cttgtccctg attcatttag
    22801 agcttaccga caagaagtct ttgtcggaaa aatgctcgat atcaagattg gaagcgagga
    22861 agtagataca actcattatg gggaagatcc attactttat attcaaaact tagtaacatg
    22921 gcatgattat cggggcaagt tagtcgggac tagcggggca acagttttta ttagagatca
    22981 atatggtacc gaggttcaat tactggacat cggaaatggg ttctatcaag atgttgcaaa
    23041 tgaattaaaa atcaaatcaa tgataactta tactcttatt gtaacaatcg gcaacgatac
    23101 aagcgaggca accacaactg tacccggcaa tttccagtta tcaaatgtta cggacggaga
    23161 tacggttgac gtaactgttc gtccttatcg aaacacgtac ggtggcgatt ttttccctta
    23221 ttggtctctt agtaattcaa ctttttttta ccgaacttta cgttctacaa gcctatttcc
    23281 tccgattatt ttgaaacaca tttattctcc tcctagcgtt atcccgctca taattgatac
    23341 aacagaagta tctattgata cagtggttgc ttcgtggatt gaagtaacaa gcttagactc
    23401 aaactacgga aaaatgtaca gtcctgaaca gacatcaact gggcccacag atttactgac
    23461 ctacttgtcc gatcaagaga ctacatcgtt atctaaacga acaaacataa aagggagaaa
    23521 cgttgcgggt gtatttggtt ccttgaatag aacaaaacgc gtaaattttt acatgaaggc
    23581 tgtaaagcag taaattaaaa cttgtaaaca tctgttgaga ggcttccgtg agaatcaagc
    23641 atacatttat tgtgatcatc atgttttccg tactttgttc ctgcaatgat gcgcctgaag
    23701 gttctcctta tttggataaa gattataatt atatcgttgg gattcttgtg ccagattctt
    23761 tcagaaccta tcgtcaagaa atctttgttg gaaaaatgtt agatattctg gttcacagca
    23821 atgaggttga tacggcacaa tatggagaag accccgtgct tcatatccag aatctggtca
    23881 catggcacga ttacaggggc aagatagtcg gcactacagg tgcgagcgta gaagtgattg
    23941 atataacaga caacgaaagt gttacgtttc aggatattgg aaatggtatc tatagagatg
    24001 tgaatgaaca attgcatgtt cacccattgc atcgatatag tttaaaagta actacgactg
    24061 agaaagcctt tactgcggaa acaactgtcc caggggattt tcaaatttct aacataaatg
    24121 aggacgatac actgactgtt gtggcaataa agcgcagcta ctattattca gcaaattacc
    24181 ctccatattg gaccagtagt caaggaaaat ttttttatag aaccgatcat ttgacgagtc
    24241 aattttttat taacttcatt aatcatgttt acgaacctcc agggtttttc acagttactc
    24301 cggatactgt aaattatcaa ttacctttca tttttagcgg ttggattcaa gtaatggctt
    24361 tagatacgaa ttacggtcgt atgtatagtc ctgaagaaac ctcaaccgct ccaattgatt
    24421 tgcttactta catttccaat caggaaacga ctcccttgcc tgaaagaaca aatattaaag
    24481 gcaaagaagc agtcggggtt tttggttcct tgaatagaac aaaacgcgtg aatttttaca
    24541 tgaaggttgt aaagtagcaa gacaatcata aaaagttgat aagctacgac gtcgcaagcg
    24601 agtgagatat tcagcccaaa taggaataaa aatttagagc cgtcaatgat ctgaaacgga
    24661 tatagaattt agaattgaag atttggggtt gagttatttg atttgaatag aaacttaatc
    24721 tccgcgttcg gcggcgatcg ggagagggac ttcgatatca cgtcgaatat gattagccga
    24781 aaaacgaatc atcatcaccg tactgatgac catcagacct gccgcaaata aaaatgtatt
    24841 tcccataccc gatgtaccga taagccacgc accgataaac ggcgcaacaa taccacgcag
    24901 accggtcagc atcgtgtgaa ttcccatata caaacccacc tgctgccgcg gcgcaaactc
    24961 catcacacag agttgccaac tgagctgcgc accgctcatg gaaatacctt gtaaaaagct
    25021 ggcaaaataa atcagtctga gatcggtcgc aaaatagaac aaaatcacat tgatcgtcgg
    25081 caaaatattg agcagaaaac gaattttgat cacaccgtat ttatcaattt gccgccccca
    25141 aaatgcaaac gtcaaaagca tcaccgcttg cggaatcacc gtcacggcga gcaccgcttc
    25201 ttcgtacgtc gcattaatcc cgtaggccgg atccgtcacg taaatagttt ttacgggatc
    25261 aatcatcagc atggcaaaac cgaaaatcgc ccaataaccc atgaaatgag caaacggttt
    25321 gtcctgccgc aaaatcgtca aggcggatcg gatcggatgc atcggcggcg tagtatggac
    25381 ggtcggaacg attttgattt ttcgcaattg ccacccaccc cacaatccga ccaaaccgat
    25441 aatggggtac acgtacacat aattatgccc gtccaaatcc agcatcaggc cgaaaacata
    25501 cgacgccaca acaaacgcga tattaaacac tgtgcgcacg atacccatca ctttgccccg
    25561 cgaatgatcg ggatagttgc tccgataaat actcgtcaga acaggcgtat tgagcgaatt
    25621 gaatgcatac gccgcgcaga gaatggccgt aaaaagccat gcattgggga tcagcgccgt
    25681 agccacggcc gtaaaaataa acaaggtacg cgcaatggca tccggcacgt acgcataatt
    25741 cattttattg gcacgttcgg catggacggc cgtaaagatg gaaaacaaca atcctagatt
    25801 ggtcgcacta tagagcaaag cgacttgaaa atcggaagca gaaaatgatt ttttgagaat
    25861 aacaccggcc acgccggcgg aaccggccag caccagccct ttggaaatac cgtacttaaa
    25921 atcatgacgg aacgtttcgt aggcatcatc gggaacacga taatgattga tgattttttt
    25981 gaacaatgaa cgcatacgat accatctatc cgaatacaaa ctttaacaaa agaaattcac
    26041 tttgtaccga cgactttttc cacgaaataa cgatgcatac gcgcatcatc ggtgatctcg
    26101 ggatgaaatg tacaaaccag tacacgcccg gaacgggcgg ccaatatgtt ttccccgacg
    26161 gacgacagga tctccgtcgc ggggcctgcc gcgacgatgc gcggtgcacg aataaacacg
    26221 gcatgcacgc ttttttcacc caaaacagga atcgtgatgt ctttttcaaa actatccact
    26281 tgccgtccgt atccgttacg ctcgacggta atatcaatta acccgaagcg gaactgcggc
    26341 gaattgacga tggttttggc aacaagtatc gaacccgcgc aggtcccgaa aattgcgccg
    26401 cctttgtcgt aaaatgcttt tatcgcatca tccatcgcaa atgcgcgcat cagcttgatc
    26461 agtgtggtac tttcaccgcc gggaatgatc aacccgtgca agccctccag ttcggacggt
    26521 ttgcgtacct gcacggcatc ggcgccgaca gactgtgtca tcttctgatg tgcctcaaaa
    26581 tcaccttgca acgccagcac accaattttt ttttgcataa acttgatctc ttaattattc
    26641 gtgttcaccg gccatgccga ccatatactg cgcgatcaca tcggtcgccg tttcccactc
    26701 cggcagaggc gactcccagg tgcgatcctg tgcgcgccgg agatatatca tttggctcat
    26761 atgataaaga taatgctgcg ggatatgcgc catcacagcg cccaacgatt tgtacggcac
    26821 tttgtccgtc gccgtggttc gtcgatccaa taacgcatca gactccaatc cgtcgagaat
    26881 ggcttcatat tgccccaacg cctgttgcaa catagtttgg agttgcgtga ctgtcgggcg
    26941 ttttatgtcg gctacatccg gcgctatatc atggcgtttg taaaaccaca tgtcaacgcg
    27001 aatgaggtga cgcatcatgt ctccgatcga acgcgattcg gccgccggtt gccagtacaa
    27061 ctgttcttcc gtgataccgt ctaaggtttt gatcaggcct gcgcgtgcga cgcggtgcac
    27121 ccggcgaagt ttttctattt tatctttcat acttatatcc ggtgatcggt taccatcccc
    27181 gattggctaa caattcactg gcggcaagat tgtccacatt gatgccgacc atcgctgcgc
    27241 cgagcgactt cgacacttcg gccaatatcg caggatcttt gaaatgcgtc gccgctttga
    27301 cgatcgcttc cgcacgtttg accgggtttc cggatttgaa aatgcccgat ccgacaaata
    27361 ccgcttccgc gccgagttgc atcacgagtg cggcatcggc aggcgtagcg ataccgcccg
    27421 ctgaaaaatt gggtacaggc agtttgccgt tttttgcaac ccattggagc agttcaaacg
    27481 gtgcacccat cgtttttgct tcgctcatca gttcgtccgt acccatcacg gtcagttttt
    27541 tgatatgccc gatgatcgtg cggatatggc gcacagcttc gaccacgttt ccggtacccg
    27601 cttcgccttt ggtacgaatc atcgccgccc cttcgccgat acgacgcagc gcttcgccga
    27661 gattggtcgc accacaaacg aaaggtactt tgtaatcatg tttgtatatg tggttagctt
    27721 catccgccgg cgtaagtact tcactttcgt caataaaatc cacacccagc gattcgagga
    27781 tttgcgcttc gacaaagtgg ccgatgcgtg ctttggccat gacgggaata ctgaccgcat
    27841 ccataatacc gagtatcatt tccgggtcgc tcatacgcgc cacgccgcca tcacgacgaa
    27901 tgtctgccgg aacgcgttcc aacgccatca cggcgcatgc gcccgcatct tcggcgattt
    27961 tagcctgctc gggattgacc acgtccataa tgacgccgcc cttcatcatc tcggccaaac
    28021 cgactttgac tttgaatgtc cctttgagaa tattgtcttc tccttgtgta cttgctgtca
    28081 tgatatattc ctttcgattt tttaagtgcg caaatataat tttttttaat tcagggtcaa
    28141 ttatgatcct caactatcgt ttgaacagcg gttcgtgcgc gcgaacgata cacattaaca
    28201 aaatacacac cgatgagtat cagaacgcag ccgatcatcg cacgtacagt caacgcttct
    28261 ccgcggacaa gccagccgag tatcacggcg actaagggcg tagcaacagg tatgatcgcc
    28321 gcttccacga cggaaacttt tttgatcacc cacatataca cgacaaaggc gaccgccgag
    28381 ccgaatacgc cgaggtagaa aatgctcgcg acggattgcg gtgtccagtg cagacgcgac
    28441 gtatcttcgg taagtaaccc tgcagctaaa aggcaaacgg cgcagatgac catttgtacc
    28501 gttacgttga cgacgggatg aatgccatcc gcatgttttt tggcatacac gctgataacg
    28561 gcgccacaca aggccgctaa aatgatggaa acatcgccca tccatgacgt accgatatgg
    28621 tgaatatcac gcagaaaaac aaccgtgact ccggcaaaac taaccacggt accgaaaatt
    28681 ttgactcccg tgattttatg ttccgccagc cacaggtatc cgagtatgat gacaaaaaac
    28741 ggcatggttg caaaaataat cgcaccgata cccgaatcaa tatactgttc accccagaat
    28801 acaaaggcat aatccagcat ttgaaaaaaa ccaagaaaca acatgacatg ccacgaatgc
    28861 cggtcgcggg gaaaccgaat acgttgaaag aacatgagcg caagtaacaa aaccgatgcg
    28921 ataacaaaac gaattccggc aaataaaaac ggcggtaaat cttcaagacc gattttgatg
    28981 gcgagccagg tcgaacccca gatcaatgta caaatcgcaa ttgcgatgtg cggccagtaa
    29041 tgtttgtgtg acgacaaaag aacctcgtat actttctatg tatttttttc tatttaatct
    29101 ttttttatcc ggatcacatc gccggcttgt accgtacctt cctgtaaaac ctcggcataa
    29161 aatccgcccg ttccccaatg cggcgactcc ggattaccgt cccgggccaa agcgtcgtac
    29221 agatgcgtcc cgatatctgc tccgtatact ttgatcgtat tgcacggcac ccgcagcttg
    29281 gtgatgcgta gtataagttt tcctccgact aaaaacgtat cgccaaaacg aatcggcgcg
    29341 tacgctaatc cctccgtcgt aaaattttct cctaaagcgc cggggaaaac aggataaccg
    29401 agttggacca acgattgata cgtttcgttc gcaatcatca ggacgacttg gcgcggtccg
    29461 ccgtgcacat ttttgttatt ccagtcatca ccttgtacgc gttcgctact gacatacgcc
    29521 gatgcggtcg gttttttagg gatgccgccc gacgaaatat tgatctgttt gatgatacct
    29581 tgcatacgga ttattctctg caaaatgtga tgtaatcggt ttgtgccggc gttcctccgg
    29641 ctttacgcac ttccatcgcg atctgcccgc gatgataggt cgagtgattg agtatatgcg
    29701 ttaaaatatc atgcacggtg ctttgatagg gctgaccatt cattgtttta tacgttatcg
    29761 tacgttgcaa aacttcctca ctcaattcat gaacaaactt gtcccaattt gaatttattt
    29821 ctgaaagaac atctaaacac cgtgtgatcg gtaaatcact ccataccgtg gtaatatgtt
    29881 tatgctcgcc atgaattcgc tgataccata gaactttaga ttcgatcaga tgtgaaaaaa
    29941 gttgtgacgc acggcctcga cctgccggta aagccatgat ggaatcggat actaaacgat
    30001 ccgcccagat ttcgtactgc cagcgtttaa taaagtcttt tgtgttcatc ataattatcc
    30061 tacatcaccg acgccagtgc ggagtccgat tcgacccacg gcgcactgcc tatttttttc
    30121 ttaataatat ctccgagtat cgcgatacct tcatgaatat gttccgtcgt ttgcgaaaca
    30181 aaacccaaac gcataccgcc accggcatcc gggcgcacag aaaaaaaccg cttgggcgta
    30241 aaaagcacac gccgctgacg ggcttccatc agtacatcgt actcatccat accgtccggt
    30301 aaatccgccc actcaaaaag acctccggca ggaacatgga aacgcacttc ctccggaaag
    30361 tactgacggg acgcggtgat catcgcatcg cgccgctctt tgacaacttt catttttctt
    30421 ttcagatagc gatcgaaatg cccggcttca caaaactcca atattgcgcc ctgaatcaaa
    30481 taattgtttt gccagtccgt gagacgtttg gcgtaattca gtttattttg aaacgcgcga
    30541 ttggaagtaa tcacccaacc cagacgaatg ccgtgaaata atatttttga aaaactgcca
    30601 agcgcgatga caataccttc gtcgtctagt gctgcaatcg gcacgatctc ctcgccctcc
    30661 atgcgcagtt cgttggcgta ttgatcttcg acaatcggta tttcgtaatg atacgccagt
    30721 tccaaaagat gtttacgccg atccagaggc atcgtccgac ccgtaggatt ttggaatgtc
    30781 ggtatcgtat aaatcatttt tactttttgc cgtgcgagta tcgtttcaag aatatccatg
    30841 cgcataccct gatcgtccat gggaatgccg atgatattgg cgccgagcga tttgaatatg
    30901 cgccgcgcac cgtgataggt tacttcttcc gtgatcaccg tatcgcccgg attgatcagg
    30961 atccgtgcga tcagatctat gccttgctga atgccgctga ccatcgtgat gtccgagctc
    31021 tgcacggtaa gcccgcgccg cacaagaaac ttcggcaaat attcgacgaa cggtcgaaac
    31081 ccctgcgtat cacccatctc c
//
LOCUS       DAOQNQ010000008        30688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_468_length_30688_cov_6.415043, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000008 DAOQNQ010000000
VERSION     DAOQNQ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 30688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30688
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_468_length_30688_cov_6.415043"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..3157)
                     /locus_tag="PK754_01140"
     CDS             complement(80..3157)
                     /locus_tag="PK754_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB08139.1"
                     /translation="MRYIRVYLFVMIFAGGLRSYLSAQEISLSGDLQPFSVTAPGAIS
                     DEFSYAVSATGLSGSLLIHAPHGFEISTTSGGVFGSNITLPETGGEIELTTIYVRFAP
                     SVTGSYDSVIVHESGVTQTLAVTGLCAFSEPTLPPSDVVFSGIDSSAMTITWQGGDGD
                     YHLVLVRADALIELAPNDGQSITANSTFGSGTDIGGNTYAVFAGSGTSVSINGLSRGV
                     NYYFSVFTYNGTGNDADYLTDAAVGEQTTLSRPPERIQMIRDTTFAEDSGTRTIIADL
                     HDIFSDPENNLLTFSAGTLVSGADAWVTDNTLEFFSSEDSSGLFEVIVEAQDNSGNFV
                     YDTLLVTVTQQNDAPKLATPIGPKNLQEDFGKQLLAYLPLVFHEADGEAMTFSIHTPS
                     PYAALMISGDSLYALSQNNLNGQSQVIIEASDPTPLSNRDTFQLQIIPINDPPARTSV
                     LRDTSFAEDFSYRPLVYLPDYYSDPDDGVINFSSFVVNTGVEGYILNDSLYVEGNEDF
                     FGSAALEIRASDGNTEIRDTIFFTITPVNDAPSRIFIARDTSVQEDAARTAILYLPMI
                     FLEPDLEPVSYGVALSNIKSVAAISGDTLYVTPLKDSTGSVEVYLSATDPTDLSAKDT
                     LVLQIVPVNDRPVRLATISDTTLPQNFGKVLIKKLSTVFYDADHTALSFSFVKFADGV
                     DPLISNDSLYLRSTVDFFGNVSVRVIASDGEFTVSDTFRVVVQDNTAPVAFLAALSSP
                     LLQRIRFALGSNETIASAALTANGQVIDIESTNGVYFGDYSIESVGVIPVVGEVFDLA
                     GNKDTIRRSYTVTSLDKKTTYDNFVFEGNQRGYLVLDQAETGNVPTLLRPLSNAVDVL
                     NTASSAEVTVHVRYQDHLSALRAADENFDEGRIGVYAYENHQWQRLSGEGRFGAVETR
                     IHKNVRIAVFYDPEYIMQPKTFALDQNYPNPFNPSTTIRFAISQPGHTTLKVYNMLGQ
                     EVRTLFSEYKPAGNFETIWDGKNEKGLAVASGVYLYRLSSGSFTQTRRMVLVK"
     gene            complement(3225..4178)
                     /locus_tag="PK754_01145"
     CDS             complement(3225..4178)
                     /locus_tag="PK754_01145"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNB08140.1"
                     /translation="MRKYQFNIPANERPKRLDHFVTHAIENLSRSRAQQLVSENMVSV
                     NGKFVAKSYMIRPGDRIEVILPLPEKVEAQAEDIPIRVVYEDDDILVVNKSAGMVVHP
                     AYANYTGTLVNALLHHVQDLSGINGELRPGIVHRIDKDTSGLLLVAKHDRAHRHLSKL
                     FKDHNIEREYWALVWGKPKKKKDTIETLIGRSTKDRKKFAVTKEGKNAITHYELITAY
                     ESVSLIRCRLETGRTHQIRVHMNYIGHPILGDKTYGGDNPNLAGGEKKKKQHAENILA
                     LIPRQALHAKTLGFIHPTTKKMMRFDSELPDDFNAVLQLIK"
     gene            4256..5005
                     /locus_tag="PK754_01150"
     CDS             4256..5005
                     /locus_tag="PK754_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08141.1"
                     /translation="MAHYPVYFSDSTLALHRSDYHSLFGNDAPVVQETVRIQIGDTAL
                     TVAVTLSDSNALCLTPQDSFRLKLNPSILTSTPTPDFLKIVIMVHARGKETRFNGTLQ
                     LIERELLLQKHEMTQLKLIPGQVVFLAARKGRDTIFGGVRVIEVSGANRARIPRDEAQ
                     GALLQENDLLYLTGLGTPNSIPVSETPVLPERFLALDLKPGKSGTSWITEKDVWQAIL
                     KHKKIWVPKNARLTPAAADLGKSHRIFEFEA"
     gene            5002..5586
                     /gene="pnuC"
                     /locus_tag="PK754_01155"
     CDS             5002..5586
                     /gene="pnuC"
                     /locus_tag="PK754_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNB08142.1"
                     /translation="MTLDWYEIFGFVTGVLCVALLIRQNIWNWPVAIGNAVFYIIVFY
                     NARLYADVVLQFFFIGINAYGWIVWLRRGPQGETLHVNSMDKVAWLWSLLAIAAGTTS
                     LGAWLHYNTDAALPYADAFGTATSLTAQFLMARKKWENWILWIAINIWYIGLYFVRDL
                     YLTQVLYVIYLGLAIAGLIEWRRAFRNPKQSLTN"
     gene            5608..6180
                     /locus_tag="PK754_01160"
     CDS             5608..6180
                     /locus_tag="PK754_01160"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNB08143.1"
                     /translation="MREQQYDSESFVQIDFTKQRIALNTYDTCTFERCDFSKSDLAEV
                     RFLDCRFIGCNFTLCSVGNTVFGNAVFQECKMTGINFEKANTALFSASFIQCDLSSSL
                     FTKIKLRKTQFDNCILKSVDFTGCDLTDAKLSASDLAGAHFENTILEKADLRTAFNYS
                     IDPDINRIRKARFSQSGLHGLLEKYGIEVS"
     gene            6204..6785
                     /locus_tag="PK754_01165"
     CDS             6204..6785
                     /locus_tag="PK754_01165"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNB08144.1"
                     /translation="MTTTSKTKKQRCWWCGDDPLYCEYHDKEWGVPVHDDQKIFEFLI
                     LEGFQAGLSWITILRKRDNFRKAFKQFDPKKVARFSKADVIRLMQDTGIVRNRLKIEG
                     AINNAKCFLEVQKEFGTFDRYIWSFTGYKTLRNPKRSRENIIATSPESDALSADLKLR
                     GFKFVGSTVIYAHMQASGMVDDHVKGCWKHKKR"
     gene            complement(6843..9059)
                     /locus_tag="PK754_01170"
     CDS             complement(6843..9059)
                     /locus_tag="PK754_01170"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNB08145.1"
                     /translation="MGIGINIEVGNFIIFQSILSKKSFMRFTLIVLALLLWCSPIYAQ
                     NENPADQRIDSLKEAFEREPSSQAIMEALGRAYAETQSYEAIIDLIKPVIEDTSTNNV
                     LIKLYVGARIKQLQTQSAGPKLLQQLLGGADTKEKKLINRVMQRDPEDAEAQLFLAEL
                     HKLADEPVYAENIYRRMMKNPRVISGPWFVSVWTAYSTLLIDQERFTEANSILTHAAG
                     TEKSSGWFELQMAQVTLETGETARGTRLFFQGLDALRDESQVHQLFEEAWAIAARTEL
                     EQWNTLNTLDAKKDFLRRFWSKRTPNLADSANAKLAENYKRLRHVKRYYARRTFPGYD
                     ERGLVFMRMGKPDHFYTDTRTESWVYESANAHFDFVDPGSGYYEMRPLTDALSLSGSA
                     AGQFQELKAMMEARRNLHPFYEQTAARLQAREDAISVERSRSNAFRNIISETEIRLGR
                     EGTNKPGLQKSETLDKIPSVSLVASYACFKGKNKSTRVDFYYMIPIRELQTLRIADSS
                     STAFLFANARILDTNYREITTLHREFKIAVVDRDAPLFVIDELRTLLQPGTYILGLEL
                     INNNNERKGVYRFDVEVRDFIGANLSISDIQPAVSVTVGLLEDKFIKPDTRIRVVPFA
                     GDHFDGRKPLSVYYEINNLTRDLKGVTSYEISYTLAPAQRGNILTRAVGKLFGSSKAT
                     ITSVTQKTGTLPTEREYVGFDISELPEGECVLTITVKDLLSGQTASSERFFWVNKK"
     gene            complement(9028..10350)
                     /locus_tag="PK754_01175"
     CDS             complement(9028..10350)
                     /locus_tag="PK754_01175"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNB08146.1"
                     /translation="MTEKKELAQSIALWDAIMLNAGSMIGSGIYIVPAAVAGYLQGSV
                     WIMTAWVVGGLVSMLGALCLAELGAMMPKAGGIYIYLSRAFSPMWGFLYGWANFAVIM
                     TGSIAAVAVGFATYLGYFIPFTPDEIKAVAIASIIFLTAINVVGILFGVAVQNFMTLL
                     KIVSLGILIIIPIVVPGGSVENFTPLFSDPNSGHWIGAFGLATIAVLWAFDGWIEITY
                     VAGEVRDPQKNVPRALVVSTALIIGLYVLINMAYLYVLPADQLRASSLVASDMAKILM
                     GPEGAAFVAGSVMISMFGANNGFVLSGARIYYAMAREGRFFEKIGDVHPRFGTPAVSI
                     IAQGVWSCGLVFTGTFEQLTTYVVFASWFFYAMACMAVIVLRKKDPQTERPYRAWGYP
                     WTPIVFIAFAAFLIGNAVMEDLRSSLIGVGIIITGLPLYGYWNQHRSR"
     gene            complement(10372..11499)
                     /locus_tag="PK754_01180"
     CDS             complement(10372..11499)
                     /locus_tag="PK754_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNB08147.1"
                     /translation="MLPDFDASQFMIEPLERASTIPSSWYTDARFHAVDREFIFSKSW
                     QYVGHVSQVAEAGQYLTATVADNPVVVLRDTENHLRGFYNVCRHRGGPLVTDARGTCN
                     KVLQCQYHGWTYKTDGSLRGIPRWNYVDLFDKKDYGLVPLQVDVWGGLVFVSLEKEPM
                     PLTKLFQGAQERISRKAYDADLERRLFSDLTFYKRIVYPVHANWKVYADNYLEGYHLP
                     FVHPELTSMLDYAQYVTETYEYYSIQYSPFSEEGNVYKTNEGEALYYFVWPNFMLNIL
                     PGRLQVNRIIPDGHERCHVIFDYFYADLSTEAKRAMAEDDIAYSDKVQKEDCDICEHV
                     HKGLMSDVYDRGRFSVECENGVYHFQNLLKKNYQSVSQDNR"
     gene            11691..12059
                     /locus_tag="PK754_01185"
     CDS             11691..12059
                     /locus_tag="PK754_01185"
                     /inference="COORDINATES: protein motif:HMM:NF033196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB08148.1"
                     /translation="MKLRWFISVMTVLIFGSWYGFVSCQTNRPKNIKVLDELKDATIE
                     EIKESMRLYTLSMGVECDYCHNKDDYASDEKKEKNISRDMMRMVRTLNESTFKNAKEN
                     VTCYTCHRGAVKLKNIPPGL"
     gene            12193..12543
                     /locus_tag="PK754_01190"
     CDS             12193..12543
                     /locus_tag="PK754_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08149.1"
                     /translation="MMRTFLHTAFSKALILTYIFIGYLAITTTDKPQCVIYAQHVMQT
                     DPTTVPSSVTIKVFAARHIQPEKLSRSIAYVSLFDFVIPPACATLLRVVKSTHFTSYN
                     GFSIEQPARAPPIA"
     gene            12925..13050
                     /locus_tag="PK754_01195"
     CDS             12925..13050
                     /locus_tag="PK754_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08150.1"
                     /translation="MKTHTQLTTETKPKKDYLFVSLMALIGLSILAFILRLAGLI"
     gene            13097..14437
                     /locus_tag="PK754_01200"
     CDS             13097..14437
                     /locus_tag="PK754_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HNB08151.1"
                     /translation="MSEESKVNRFFSRPVMIGIFSILLFISALLFVRDPLFQQSSNGQ
                     WLFLSIPVEFILFGLTLLCVALFHHYVLQVALTGLAVILTYKLSFTSFDVVAHIHHEW
                     AIVVNLLGLLVGFAILAKHFEESHIPEILPKHLPNGMAGPLVLLCITFVLSSFLDNIA
                     AAMIGGTIALTVFRGNVHIGFLAAIVAASNAGGAGSVLGDTTTTMMWIDGVSPWDVLH
                     AYYGSIGAFAFFAVVASIQQNKLQTIIRAEGSENHSIDWGKILVVALILVGAIATNIL
                     LDFPALGVWAAIFIGAFIRETAWHEIPVALKGSVFLVSLVLSASMMPVDQLPPASWFS
                     AFILGFVSSVFDNIPLTKLALDQGGYDWGVLAYAVGYGGSMIWFGSSAGVAISGIFPK
                     AKSVVFWLKDGWHVAVGYVIGFFIMLALWGWEPHEKHKIEKGESHKQPSTEQTH"
     gene            14605..15987
                     /locus_tag="PK754_01205"
     CDS             14605..15987
                     /locus_tag="PK754_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB08152.1"
                     /translation="MKSRFDFFITVLCVCGVVTLSAQSASDLVQQANEKFSSAGSEKE
                     YKEAAGIYEKAAAADSTLAIAWAGLAKSHAMIGNIASLNYKDDLKIKHYKESAKYASK
                     AGKMAKENSDVQTALAMVYRQLSQRDNLMRVAQKAVALDPKNAEAYDLWADAYSEDYF
                     PKNADIDSSILIRKKAVEANPNFAKGYRGLGDDYASKGDLEQAESYFTKAIAINPQHA
                     GSHDRLGRLYYLQGEFEKAKTEFNTAIKLDPRSTFAYTHLADVLADEGNFKEAFLKFT
                     YAVDLNPNAAYAHASLSWMYLTAKDKSFRNVGKAIEYGAMAAELTNNTNPEFLAVAAE
                     AYYEASFNKSELRDSAINRLKTALSIEPDNFEYQETLGRMNAKEKSREYVYAWKRGER
                     LLKHNRINDAINEFITSEEKNDKFVPNLISYAKALKKKGDEATMKVYLEKARKADKSG
                     KYTTSISSVQ"
     gene            16036..17892
                     /locus_tag="PK754_01210"
     CDS             16036..17892
                     /locus_tag="PK754_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB08153.1"
                     /translation="MRACMNLFFFAMMISASLWAQLKVAVVPFYNGTMLSKNEWLGHG
                     FCESLNTDLSEEKSVLIMDRPDVLAEIKMNGFKLLDMLDEQKALTVARSLEVDKIIIG
                     SFDIPGGATPSIKVQARFIDVKSGKADTKNAYTAEGKYSMTNIYAMYGQIAARALVSF
                     GKRAADETAVTGKGSINVDAYEPYIKGILQYDESSTVEDYLASIALLQQAIKTDSSFA
                     MAYAGMAKAYAKIGRIQDINFKADEKTESYKKALDAGLKAAKLDKNLAVAWTALALTY
                     RELKERDQLIEAARKAVALKPSQYDAYDMLADAFSANFFPAHKNNDSSVFYRKKSVEY
                     NNKFASGYRGLGRDYFDKGDYKNAEEAYQRAITLNPKHAGSRDLLGQVYTIQGQYEEA
                     KQQFREAIKLDAKSAFAHTHLADVLAMEKNYAEAVTSYQSAIAINAKFALAYNGLAWI
                     YIAAKDKTLRIPAKAVENAQAAVQHSDSKNASYLYTLSEAHFSAGTHDAALEAIGKAI
                     ALDPHNNDYAETQSRITKKEKNSDYVFTLRRGMMLMKQGRQDEGVVEFEEANKLSPQN
                     VYILFTLAKYYDSKKEFKKAFEYYYRARSCDWDKKYSKSIQERMNELAPYGN"
     gene            17976..18455
                     /locus_tag="PK754_01215"
     CDS             17976..18455
                     /locus_tag="PK754_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNB08154.1"
                     /translation="MHPNEETINRFYRAFQNKDFSVMQHSYHDDAVFYDPVFEDLTAA
                     EVRTMWEMLCKRGKDLVLEYRIIHADDQRGSAEWIATYTFSATGRKVINRIHADFTFR
                     ENKIMSHKDTFDLYRWAKQAFGFSGVLLGWTGWMQNKIRSTAKNNLQRFMGKSSPVS"
     gene            complement(18461..19324)
                     /locus_tag="PK754_01220"
     CDS             complement(18461..19324)
                     /locus_tag="PK754_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNB08155.1"
                     /translation="MAVQTITVLGSGIMGNGIAHVAAQAGYKTILHDVSLDALNKALN
                     TISKNLDKGIELGKISAQDKEATLSRISVEQHLEAAVAPSDFVIEAVPEKIDLKIKIY
                     TTLDKTCKPETVFATNTSSLSITEIAAVTQRPQKCIGMHFFNPVHKMKLVEIIKALET
                     DEATIKATQDVAIAMGKEIVTIKESPGFITSRINALIGNEAFYMLQEGIGSAKDIDTA
                     LKLGLNHPMGPFEMVDLVGLDTRLSILEYLHKSLGEKYKPCPLMVQYVKAGRLGRKTG
                     KGVHEYPDVKK"
     gene            19584..20873
                     /locus_tag="PK754_01225"
     CDS             19584..20873
                     /locus_tag="PK754_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB08156.1"
                     /translation="MTIEPYFTTGQHNRFSEMAKGLLGSEILKIASDIRAMVAKGQKI
                     GNLTVGDFSPAEFRIPQFMEDAIRRHYAQGQTNYPPSDGMLELRNAIVGFYKTWLDVE
                     YPVKSVLVASGARPLIYSLFRAIVDPGDKVVYPVPTWNNNNFVQMLGAQGVPVHCHEE
                     HAFLPTVDLLKPHLHDARLICLNSPLNPSGTAFSRQELEAICDMILEENKRRTTEDAL
                     YLMYDQVYWMLTFGKTEHFNPVHLRPEIAPYTVMVDAVSKAFAATGVRVGWCVGPPDI
                     IERMSAILGHVGAWAPRAEQMATAELLNNKDEIVKYHSVMKVGVQDRLNALYEGLSAM
                     AKDGLPIKTIVPMGAIYLSVQFNFLGRKKPDGTVFTNSEEIRQYLLFESQIGVVPFKA
                     FGYQHDTGWFRLSVGAVSMKDIEEALPRLRRILEKLV"
     gene            20881..21555
                     /locus_tag="PK754_01230"
     CDS             20881..21555
                     /locus_tag="PK754_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNB08157.1"
                     /translation="MDKNSMTIKPGLAAPELRISEWIQGTPIQLSELQGRVVIIEVFQ
                     VNCPGCFLYGLPEILEIAAKHKNEPLTVIGLATAFEDFDINTKDNLQKLLQTGELVGE
                     TARVLKEHDWATDNKLRYTIPIPVAMDRLGEGSRLDTVSQIERIIERDITNFDRLSYG
                     EQGRIRESILKYLNERTLVPETFDLYNLSGTPSSIVIDKKGILRHIQFGSTGNLEKII
                     RPLLTE"
     gene            21645..22106
                     /locus_tag="PK754_01235"
     CDS             21645..22106
                     /locus_tag="PK754_01235"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HNB08158.1"
                     /translation="MKTIEEAFIHEGYLSIPFSYNGAGHPIIKVTVANGFQTNILLDT
                     GASANLLDLEFAQENGLNLTPTGEKGGGAGGLTLDVFTVDKLTLEINGKKLQFTDFLS
                     MDFTTIKQSLITNGLEADFQGILGFGFFKKTKCFIDYSSDRIFIQNNSYIE"
     gene            22227..23624
                     /locus_tag="PK754_01240"
     CDS             22227..23624
                     /locus_tag="PK754_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNB08159.1"
                     /translation="MTSLYRPLGNTGYTCHPLGFGSYRINEDNPTHERALRSYIQHGG
                     NLIDTSANYGDGASETLIGKILSDINRSKMIVVSKGGYIQGQNLALAQKHDFPEVIKV
                     QDNLWHCIHPEFLETQITRSLQRLQSDYIDIYLLHNPEYFINHQSNFGPLDDTVHDAF
                     YRRVRRAFEYLESQVALGRIRYYGISSNNFGYHAQDRARTDVSRCWAMAEEVSPHHHF
                     RVVQMPMNPYEAGGAIYPTQNGLTPLEFCIKHNIGVLINRPLNAFHDNRLIRIADYVT
                     PGQNKPDISTLRDEMQPLINLENEFKLRFNGSPFGDEEQGLVSYLLYIADDLPSKEHW
                     NTVIDRYIVPPVTQWLRDQSALYSDSIVWNAWQENFVKAVNLTLDRVEKYVAYSDQKV
                     SDRVRQRLMDSGYPSSDASLSQMALSLLIQTQGVSCVLNGMRTESYVKDAMGALDLPA
                     VDAKHILERFDQSIR"
     gene            23625..24599
                     /locus_tag="PK754_01245"
     CDS             23625..24599
                     /locus_tag="PK754_01245"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008632316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNB08160.1"
                     /translation="MKTLKLKPTDANIRKAARILQKGGLVALPTETVYGLAANAMNTK
                     AVRRIFKAKGRPSDNPLIVHIADRRMLSTLVDTIPEHARLLMRHFWPGPLTLVLRHNG
                     TLPLTVTAGLSTVAVRLPGLALTRKIIRLSGVPLAAPSANRSGRPSPTQSEHVLHDLD
                     GRIEAVIEGGIARVGIESTVVDVTGKIPVILRPGHITIEDIRRVCDHARLSHHRRHTS
                     VRSPGMKYRHYAPDTPLQIITPEKIEAYAKRNTHLRLGIMTMRRGLHIPNATVIYIGN
                     TPTRAARHVFAALRKLDGKVDRIVCEALEEKGIGHAVMNRLKKAASKK"
     gene            24621..25181
                     /locus_tag="PK754_01250"
     CDS             24621..25181
                     /locus_tag="PK754_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNB08161.1"
                     /translation="MHREAAWELLCEYTKGESLRKHALAVECAVRAYAVKFGEDVELW
                     SVTALLHDFDYEMFPDPVAPNGHPYKGNEILKDKGYPDDVRRAIMSHADYTGVSRDSL
                     LEKTLFACDELAGFITAVAYVRPTKSVKEVEVKSVRKKMKDKAFAAKVNRDDIINGAA
                     ALGIDLDEHIAFVIKALQDNADSLGL"
     gene            complement(25190..28549)
                     /locus_tag="PK754_01255"
     CDS             complement(25190..28549)
                     /locus_tag="PK754_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB08162.1"
                     /translation="MFGLAGLSVSVFSQQASFKRISIEEGLSQSAVHCLLQDKLGFIW
                     LGTQDGLNRYDGYGFKIFRHDPQDSLSISDNWIQTIYEDRNGKLWIGTDGDGLNCYDR
                     QTQSFVRYSARKGKPGSLPHGRVWAISEDVHGTLWVGTIGGGLATLNQTTHTFTIYDT
                     SNTSLASNEIMSLRATQDGKLWVGTFGGGLYRMDPQTMQMQVYRHNPRNASSLADDRI
                     RTIIEDQHGTLWIGTYNGLSILDRSDQEQGRFRSVQDSVLTSRAVFALTENTDETVWV
                     GTFGSGIRTIDRTGRIVSSYKYDAKDATSLGDNSLYAFFCDRSGRMWIGTYSGGVNVF
                     DTRKKDFRSMRHDPHNLASISDNRVRSIWKDTDGTMWLGTFKGLNRLDSRTGYISFFT
                     SESGASSSLPENRIRIIRRDTKGRLWIGTSAAGLAVMDSLTNKISIYKHDPSSENSLP
                     NNGVWSIAEDPQGTMWFGTTGGGFCRFDEKKKNFERVQPASGEPGYFSNDPVLALHKG
                     RDGKLWIGTASGLKSFVPTERRFIDLDFATNTPRSSSLSRNRVRSIYEDHDGVLWVGT
                     DVGLQRWDPDSSTLRLYNHRQGLISDVIYGILEDKQRRLWLSTNYGIARFDPQSESFR
                     NYDMTDGLANNEYNQNAYFRSHDGWFYFGGINGLDYFHPDSIKDNTDKFAVVITGLEL
                     FNQTVEVGKSYNGFILPKAISELEELVLSYRESVIGLKFSALTFTQPEKNRYAYKMEG
                     FDEDWNYIDAGRRIATYTNLDPGTYRFQVRATNHDGIWNENTCVLTIRITPPWWQTWW
                     AYAVYTGLVVLGLMLFVRIRIQNERKRHELEKARELAKINQELNDTIVRLRDTQAQLV
                     QSEKMASLGQMTAGIAHEINNPLTFVYGNFEGLKDSVEKMRRRYAEGSATIESTALPS
                     PSETHGWMHELLEQINNGITGSRRIRDIVENLRKFARIGESGYKETDINADLLRIIDL
                     FVKQHNDIRIDTSLGEGCIVYVNASEMNQVFFNVLTNAVQAIQEAETQAVIRSGSGII
                     CVQTASALAHDQAWISIVIKDNGVGIAPDDLKKIFDPFFTTRPVGQGRGLGLSEAYGI
                     VHKHGGHIDVSSEVGKGTSVRIVLPKGHHPV"
     gene            complement(28792..29919)
                     /locus_tag="PK754_01260"
     CDS             complement(28792..29919)
                     /locus_tag="PK754_01260"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB08163.1"
                     /translation="MKSTETSHDNARVKRLRSYHILDTESEQRFDDLARLASHICGTP
                     VAMITLIDSDRQWVKSSVGFPTVHLPRDVAFCSHTILGDELLIVRDASEDKRFEHHPL
                     VTGATQLRFYAGMPFFSFDGFALGSLCVLDNKPRDLSDEQKETLRALAHQIEDQLELR
                     RHLLNMQSQIDEKTMADEETRRREMYIRKILETSRDGVLVEENEKVVFMNTAYAKLFG
                     RTSADELMGLHLSELAAPHDLPRLLEYGRRRSLGEEVPSLYDFQIRNKNGDFVDVEAS
                     VSVFTHEDKKYIITFARDIMERKRHEKEREKLIAELQAALGEVKTLSGLLPICASCKK
                     IRDDKGYWNQLEYYIEANTKAMLTHGICPECVQKLYPEEDA"
     gene            complement(29937..30245)
                     /locus_tag="PK754_01265"
     CDS             complement(29937..30245)
                     /locus_tag="PK754_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNB08164.1"
                     /translation="MKTRHRTNRISVIREFMQIPGVGKSIAQDFWDMGFRSVGELAKK
                     NPEKLYEDFCTRRGQIVDRCMLYVFRCAVYYALHPDPEPEKLKWWNWSDKKISSLSIH
                     "
     gene            complement(30252..>30688)
                     /locus_tag="PK754_01270"
     CDS             complement(30252..>30688)
                     /locus_tag="PK754_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB08165.1"
                     /translation="RMRGVEASIHVFYKQYLNASAGYTLLDARDISKDAGTRDVGYTS
                     GNHDLPYKAKHSFNFNIDAGYEPVSLNLNGRYNSAIREVSIYPGSEPDAFFVLNGKFS
                     VEFLPKRSVYISAQNITNTQYEEIERYRMPGRSYMIGCSMQF"
BASE COUNT         8517 a         7511 c         6581 g         8079 t
ORIGIN      
        1 gccgtttcat ctttggacgg gcaacctgta atccaaaaaa cggaaaccaa taaaatcaaa
       61 taacggatag ataaagtttt cattttacta aaaccattct gcgtgtttgc gtaaatgagc
      121 ccgagcttaa gcgatacaaa tatacgcccg aagctaccgc gaggcctttt tcatttttac
      181 catcccaaat tgtttcaaaa tttccagccg gtttgtattc gctgaaaagt gttcgcactt
      241 cctgcccgag catattataa acttttaacg tcgtatgtcc cggttgtgaa atcgcaaaac
      301 gaatggtcgt ggacggatta aacggattcg gataattttg atcaagggca aatgttttcg
      361 gttgcatgat atattcgggg tcataaaaca ccgcgatacg tacattttta tgaatgcgtg
      421 tttcaaccgc gccaaaacga ccttcaccgg aaaggcgctg ccattgatga ttttcgtacg
      481 catagacacc gatacgccct tcatcaaaat tttcatccgc cgcacgcaac gcacttagat
      541 gatcctgata tcggacatgc acggtaactt cagctgagga agccgtattc agtacatcca
      601 ccgcattcga aagcgggcgt aacaacgtcg ggacattgcc ggtttcagct tgatccaaaa
      661 cgagatatcc gcgttgatta ccttcaaaaa caaaattgtc gtaggttgtt tttttatcca
      721 aagaagttac cgtataactt cgacgaatcg tatccttatt tccggcaaga tcaaatactt
      781 cacccacgac cggtattaca ccgacggatt cgatactgta gtcgccgaaa tacacaccat
      841 tggttgactc gatatcaatc acctgtccgt tggcggtaag cgcggccgaa gcgatggttt
      901 cattacttcc caatgcaaac cgaatacgct gaagtaacgg cgatgataat gccgccagaa
      961 atgccaccgg cgccgtattg tcctgcacta cgacacggaa tgtatccgat accgtaaatt
     1021 caccgtcgga agcaatgacg cgcacggaaa cgttaccaaa aaaatctact gtactgcgca
     1081 aatataagga atcgttggaa atcagtggat ctacaccatc ggcaaatttg acgaatgaaa
     1141 aactcagcgc ggtatggtcc gcatcataaa atacggttga gagttttttg atcagaacct
     1201 taccgaaatt ttgcggtagc gtcgtatccg atattgtcgc caagcgtaca ggtctatcgt
     1261 taaccggtac aatttgcaag accagcgtat ctttggctga caaatcagtc ggatccgtag
     1321 ccgacaaata cacctccacc gaacccgtgg aatccttcag cggcgtaaca tataacgtat
     1381 cgcccgaaat tgccgcgacc gacttaatat tggaaagcgc tacgccatag ctgacgggtt
     1441 ccaaatccgg ttccaaaaaa atcatcggca gatacaaaat cgccgtgcgg gccgcatcct
     1501 cttgtacgct cgtatcacgg gctataaata ttctactcgg cgcatcatta actggtgtta
     1561 ttgtaaaaaa aattgtgtcc cgtatttccg tattaccatc tgaagcgcga atttcaagag
     1621 cagcgctgcc aaaaaaatct tcatttcctt ccacgtataa cgaatcgtta aggatataac
     1681 cctcaacgcc ggtattcaca acaaaagagg aaaaattaat cacaccatca tccggatcgc
     1741 tataataatc cggtagatag actaacggac gatatgaaaa atcttccgca aaactggtgt
     1801 cccgcaacac cgacgtacgc gccggcggat cgttgatcgg aatgatttga agttggaatg
     1861 tgtcacgatt gctcaaaggc gtgggatcgg aagcttcgat gattacctga ctttgaccgt
     1921 tgagattatt ttgtgacaaa gcatacaaac tgtcgccgga aatcatcaac gctgcatacg
     1981 gagacggcgt atgtattgaa aatgtcatcg cttcaccatc cgcttcatga aataccaatg
     2041 gcagataggc taacaattgt tttccaaaat cttcctgcaa attttttggc ccgatcggag
     2101 tagctaattt cggcgcatca ttttgttgcg ttaccgtgac aaggagcgta tcgtatacaa
     2161 agttaccgga attatcctgc gcttcgacga tcacttcaaa tagaccggat gaatcttcgg
     2221 aagaaaaaaa ctccagcgtg ttatctgtga cccacgcatc cgctcccgat accaatgtgc
     2281 cggcagaaaa ggtaagcaaa ttattttccg gatcggaaaa aatatcatgg agatccgcta
     2341 tgatcgtacg agtgccgcta tcttctgcaa atgtagtatc ccgtatcatt tgtatccgtt
     2401 cgggcggacg actcagcgtc gtctgttcac cgacagccgc gtccgtcaga taatccgcat
     2461 cattccccgt gccgttatac gtgaaaacag aaaaataata attcacaccg cgcgataaac
     2521 cgttgatact cacagacgtt ccgctgcctg caaaaacagc gtaggtatta ccaccaatat
     2581 ccgtaccgct gccaaacgtc gaattcgctg taatactttg gccgtcattg ggtgcaagct
     2641 cgatgagcgc atcggcgcga acaagtacca aatgatagtc gccgtcacca ccctgccaag
     2701 tgatggtcat cgctgatgaa tcaatcccgc taaatacgac atccgatggc ggcaatgtcg
     2761 gttcactgaa tgcgcacaaa cccgttacgg ccagtgtctg tgtaacaccg ctttcatgta
     2821 cgatcacact gtcatacgat ccggtaaccg agggcgcaaa acgcacataa attgtcgtaa
     2881 gctcaatttc tccgccggtt tcaggaagtg taatattgga accaaacacg cctccgctgg
     2941 ttgtagagat ttcaaatcca tgcggcgcat gtattaataa agaaccgctc aaacccgtag
     3001 ccgacacggc atacgaaaac tcatcagaaa tcgcaccggg cgctgtaacc gaaaacggtt
     3061 gcaggtcccc tgaaagcgaa atttcctgag ccgacagata cgaacgaagg ccgcccgcaa
     3121 aaatcattac aaataaatac actcgaatgt agcgcatgcg aattagttat ccgatttacg
     3181 actgtgacaa ttatacaaaa aaacaaccta ttcgggctaa aaaattattt tatcaattga
     3241 agaacggcat taaaatcatc cggcagctcc gaatcaaagc gcatcatttt tttagttgtc
     3301 ggatgaataa aacccagcgt tttggcatgc aacgcctgac gcggtatcag ggctaatata
     3361 ttttcagcgt gctgtttttt ctttttttct ccgcccgcca aattcggatt atctccgcca
     3421 tacgttttgt caccaagtat cggatgaccg atatagttca tatgcacgcg gatctgatgc
     3481 gttcgcccgg tttccagccg gcatcgaatc aatgaaacgc tttcgtacgc cgttatcaat
     3541 tcgtaatgcg tgatagcgtt tttaccttcc ttagtcacgg caaatttttt tcgatccttc
     3601 gtactccgac cgatcagggt ttcaatcgta tcttttttct ttttgggttt accccatacc
     3661 agcgcccaat attcgcgctc gatattatga tctttaaaaa gtttactcag atgacggtgc
     3721 gcccgatcat gtttggcgac taataaaaga ccgctggtgt ctttatcaat acgatgcact
     3781 atacccggac gtaactcacc gttgataccc gacaaatcct gcacatgatg cagtaaggca
     3841 ttgaccagcg taccggtata attagcatac gccggatgaa caaccatgcc ggccgattta
     3901 ttaactacca agatatcgtc atcttcatat accacacgaa taggaatatc ttcggcttgc
     3961 gcttcgactt tttccggcaa aggtaatatc acttcgatcc gatcgcccgg gcgtatcata
     4021 tacgatttgg caacaaactt accgttgaca ctgaccatgt tttccgacac cagctgttgc
     4081 gcgcgcgaac gtgaaagatt ttcgatcgcg tgggtcacaa agtgatcaag acgtttgggg
     4141 cgttcgttgg ccggtatatt gaactgatac tttcgcatgc tttcaggaat tcgccgttga
     4201 atgaatgccg ttaattgtgt aacatagtca aaacaattct gaatttcaat ttgctgtggc
     4261 acattacccg gtttattttt ccgactctac ccttgcgtta caccgctcgg attatcactc
     4321 cctttttggc aatgatgcac ctgtcgtaca ggaaaccgta cgtatccaga tcggtgatac
     4381 ggcccttacc gtcgccgtga cattatccga cagcaatgcg ctgtgtctta cgccacaaga
     4441 tagttttcga ttaaagctta atccctctat attgacatca accccgacac cggattttct
     4501 aaaaatcgtt attatggtgc atgcccgcgg aaaagagacc cgcttcaatg gtaccttgca
     4561 attgatcgaa cgcgaacttc ttttgcaaaa acacgaaatg acacagctta agctaattcc
     4621 cggccaagtg gtttttcttg ctgcgcgcaa agggcgtgat acgatttttg gcggtgtgcg
     4681 tgtcatcgaa gtcagtggcg ccaatcgggc caggatacca cgtgatgaag cacaaggcgc
     4741 tcttttacag gagaacgatc tgctttacct caccgggttg ggtacgccga attctattcc
     4801 cgtttccgaa acaccggttt tacccgaacg ttttctagcg ctggatctga aaccaggaaa
     4861 aagcggtaca tcgtggatta ccgaaaaaga tgtgtggcaa gccattctca aacacaaaaa
     4921 aatctgggtt cccaaaaatg cccgccttac tccggccgcg gcagatctcg gcaaatcaca
     4981 tcgcattttt gaatttgaag catgacgctt gattggtatg aaattttcgg atttgtaacg
     5041 ggtgtcctat gcgtcgccct cctgatccgt cagaatatct ggaactggcc tgtcgctatc
     5101 ggcaatgccg tgttctatat tatcgtattt tataatgcac gtttgtatgc cgatgtggtg
     5161 ttgcagtttt ttttcatcgg catcaatgcg tatggctgga tagtctggct gcgacgcgga
     5221 cctcaaggtg agacgctgca cgtcaactca atggataagg tcgcatggct ttggtctctg
     5281 ttggcgatag cggcaggcac gacttccctc ggagcatggc tccattataa taccgacgcc
     5341 gcactgccgt atgccgacgc cttcggaacg gccaccagtt tgacggctca atttttgatg
     5401 gcccgaaaaa aatgggaaaa ttggattttg tggatcgcca tcaatatctg gtatatcggt
     5461 ttatattttg ttcgggacct ctatcttacc caagtccttt atgtgattta tctcggcctt
     5521 gccatcgcgg gtttgatcga atggcgacgc gcttttcgca accccaagca gtcccttacc
     5581 aactaactgt tacacaggta ccatctgatg cgcgaacaac agtatgactc ggaatccttt
     5641 gtacaaattg atttcaccaa acaacgtatc gcactgaata cctacgatac ctgcacattt
     5701 gaacgatgcg atttttcgaa atcggatttg gcggaagttc gttttttaga ttgccggttc
     5761 atcggttgta attttacttt gtgcagcgtg ggcaacaccg tcttcggtaa tgctgttttt
     5821 caagaatgta aaatgacggg aattaatttc gaaaaagcca atacggcgtt attttccgct
     5881 tcatttatcc aatgcgacct cagttcatcc ttattcacca aaataaaact ccgtaaaaca
     5941 cagtttgata actgcatatt aaaatcggtt gattttacgg gatgcgatct gaccgatgca
     6001 aaattgtcgg ccagcgatct ggcgggagcg cattttgaaa ataccatact tgaaaaagcg
     6061 gatttacgta ccgcgttcaa ttattcgatt gatccggata tcaaccgcat acgaaaagcg
     6121 cgtttttcac aaagcggttt acacggtttg cttgaaaaat acggaataga agtcagttga
     6181 attatttaac ccgaccgaaa gttatgacaa caacctcgaa aacaaaaaaa caacgatgct
     6241 ggtggtgcgg cgacgaccct ttgtattgcg aatatcatga taaggaatgg ggcgtaccag
     6301 tacacgatga tcagaaaata tttgagtttt taattctcga aggatttcag gccgggttga
     6361 gttggataac gatactgcgc aaacgcgata attttcgcaa agcgttcaaa caatttgatc
     6421 ctaaaaaagt ggcacgcttc tcaaaagcgg acgtcatccg tctgatgcaa gacaccggta
     6481 tcgtgcgtaa ccgactcaaa atcgaaggtg cgatcaacaa cgccaaatgt tttctcgaag
     6541 tacaaaaaga attcggcaca tttgaccgtt atatttggtc ttttaccggg tataaaacgc
     6601 tacgcaatcc aaaacgctcc cgcgaaaata tcattgccac ttcgccggaa tcagatgcct
     6661 tgtccgctga tctgaaatta cgtggtttta aatttgtcgg ttcgaccgtc atttatgcgc
     6721 acatgcaagc ttccggaatg gtagatgatc acgtcaaagg atgctggaag cacaaaaagc
     6781 gctaaaactt tttaatcgtt gcactaactc cacctcgacc ggtaaacacg aaggcgagtt
     6841 atctatttct tattaaccca aaaaaaccgc tcggacgaag ccgtctgacc ggataacaga
     6901 tctttgacgg taatcgtcaa aacgcattcg ccttccggca attcgctgat atcaaatccc
     6961 acgtattccc gttctgtcgg caaggttccg gttttttggg tcaccgacgt gatcgtcgct
     7021 ttactgctac caaaaagttt acctacagcc cgcgttaaaa tatttccgcg ttgagccggt
     7081 gccaacgtat aactgatttc gtaggatgtc acgcctttta gatcacgtgt cagattgtta
     7141 atttcataat acaccgacag cggtttacga ccgtcaaagt gatcgccggc aaaaggcact
     7201 acacgtatac gtgtatcggg tttgataaat ttatcttcca gcaaaccaac cgtcactgaa
     7261 acagccggct gaatatcact gatggataag ttggcgccga taaaatcacg cacttccaca
     7321 tcaaatcgat acaccccttt gcgctcgttg ttattattaa tcagctcaag tcccagaata
     7381 tacgtacccg gttgcaataa cgtgcgcaac tcgtctataa caaaaagcgg cgcgtcccga
     7441 tcgacaacgg ctattttaaa ttcgcggtga agcgtagtaa tctctctgta gttggtgtcc
     7501 aaaatacgcg cattggcaaa tagaaacgct gtacttgagg aatcagctat acgcaaggtc
     7561 tgcaactcac ggatcggaat catataataa aaatcaacgc gagtagattt attttttccc
     7621 ttaaaacacg catacgatgc cacaagcgac accgaaggaa ttttgtccaa cgtttcggat
     7681 ttttgaagac ccggtttatt cgtaccttcg cgccccaaac gtatttcagt ttcactgata
     7741 atatttcgaa acgcattgct acggctgcgt tctacggata ttgcatcttc acgtgcctga
     7801 agccgcgcgg ccgtctgctc gtaaaacgga tgtaagttgc gacgtgcttc catcatcgct
     7861 ttaagttcct gaaactgtcc agccgcactt cccgacaaac tcaaagcatc ggtcaacggg
     7921 cgcatttcat aataaccgct gcccggatct acaaaatcaa aatgcgcatt ggcggattca
     7981 taaacccacg attcggttcg ggtatcggta taaaagtgat cgggtttgcc catacgcata
     8041 aaaaccaatc cgcgttcatc ataacccgga aaggtgcgtc gcgcgtaata ccgttttaca
     8101 tgacgcaaac gtttataatt ttcagcaagt ttggcattag ccgagtcggc aagattggga
     8161 gtccgttttg accaaaagcg acgaagaaaa tctttttttg catcgagcgt attcaatgta
     8221 ttccactgct caagttcggt tcgggccgcg atcgcccacg cctcttcaaa aagttgatgt
     8281 acctgagatt catcccgcag tgcatcgaga ccttgaaaaa acaatcgtgt accgcgtgct
     8341 gtttcacctg tttccaaggt cacctgagcc atctgaagtt caaaccaccc gctgcttttt
     8401 tccgtacctg cggcatgggt tagaatggaa ttggcctccg taaaacgctc ctgatcaata
     8461 agaagcgtac tatacgctgt ccaaaccgat acaaaccaag ggccggaaat gacacgaggg
     8521 tttttcatca tcctccgata gatattttcc gcataaaccg gttcatcggc caacttatgc
     8581 aactcggcta aaaaaagttg cgcctccgca tcttccggat cgcgctgcat aacgcggtta
     8641 atcagttttt tctctttggt gtccgcgccg cctaaaagtt gttgcaaaag tttgggcccg
     8701 gctgactgtg tctgaagttg tttgatgcgc gcgccgacat acagtttgat caggacattg
     8761 tttgtggatg tatcttcgat caccggcttt atcaaatcaa taatcgcttc atacgattgc
     8821 gtttcagcat acgcgcgacc caaggcctcc ataatagcct gcgaagaggg ctctcgttcg
     8881 aaggcttcct tcagcgaatc aattcgctga tccgccggat tttcattttg agcatagatc
     8941 ggggagcacc acaaaagcag agccaacaca ataagtgtaa aacgcataaa tgattttttg
     9001 gataagatag attgaaagat aataaaatta ccgacttcga tgttgattcc aatacccata
     9061 caacggcaat ccggttatga taattcccac accgatcaat gatgaacgca aatcttccat
     9121 gaccgcatta ccgatcaaaa aagccgcaaa cgcaataaac acgatcggag tccagggata
     9181 accccatgcg cggtatggtc tttcggtttg cggatctttt tttctcaaca cgataaccgc
     9241 catgcacgcc atcgcataaa aaaaccatga tgcaaatacg acgtacgtgg tgagttgctc
     9301 gaaagtacct gtgaatacta atccgcagga ccaaacaccc tgtgcgataa tcgataccgc
     9361 cggtgtacca aaacgcggat gcacatcgcc gattttttca aaaaaacggc cctcacgggc
     9421 catcgcgtaa taaatacgtg caccgctgag gacaaatccg ttattggcac caaacatgga
     9481 aatcatgacc gatcctgcga caaaagccgc cccttcgggt cccattaaaa ttttagccat
     9541 atccgatgcg accagcgaag aagcgcgcaa ctgatccgcg ggcaaaacat aaagatacgc
     9601 catattgatc aaaacataga gcccgatgat gagcgcggta gatacgacca aagcgcgagg
     9661 tacatttttt tgcggatcac gcacttcacc ggccacatag gtgatttcga tccatccgtc
     9721 aaacgcccac aacacggcaa tcgtggcaag accaaaagcg ccgatccaat gaccggaatt
     9781 tgggtcagaa aaaagaggcg tgaaattttc gaccgatccg ccgggtacta cgatcggaat
     9841 aatgatcaat attccgaggg aaacaatttt gagcagcgtc atgaagtttt gaacggccac
     9901 accaaaaagg attccgacaa cattgatggc ggtgagaaat atgatgctgg caatcgccac
     9961 cgcttttatt tcatccggtg taaacgggat aaaatatccg agatacgtcg caaaacccac
    10021 agctacggct gcaatagagc cggtcataat cactgcgaaa ttggcccatc cgtaaagaaa
    10081 accccacatc ggcgaaaaag cgcgactgag gtatatataa attcctccgg cctttggcat
    10141 catcgcaccc aattccgcca aacacaaagc acccaacata ctaaccaatc caccgacaac
    10201 ccatgccgtc atgatccaaa ccgaaccctg aagataaccc gccacggccg ccggtacgat
    10261 ataaatgccg gagccgatca tactgcccgc attgagcatg atcgcatccc ataacgcgat
    10321 ggattgtgca agttcttttt tttctgtcat tcgcagttcg actcaatcgc tttaacgatt
    10381 atcttgtgaa accgattgat agtttttttt gagtagattt tggaaatgat acacgccatt
    10441 ttcacactct accgaaaatc ggcctcggtc atacacgtcc gacataagtc ctttgtgtac
    10501 atgttcgcaa atatcacaat cttctttttg tactttatca ctataggcga tgtcatcttc
    10561 cgccatcgca cgtttggcct ctgtcgaaag atccgcgtaa aaatagtcaa agatcacatg
    10621 acaccgttcg tgaccgtcag gtatgatacg attgacttgc aaacgtcccg gtaaaatatt
    10681 gagcataaaa ttaggccaaa caaaataata caacgcttcg ccttcattgg tcttatatac
    10741 gttgccctct tcggaaaaag ggctgtactg tatcgaataa tattcatacg tctccgtcac
    10801 atactgcgca tagtcaagca tggacgtaag ttccggatga acgaatggca agtgataacc
    10861 ttccagataa ttatcggcat acactttcca attggcatgc accggataca cgatgcgttt
    10921 ataaaacgtg aggtccgaaa acaatcgccg ctccaagtcc gcgtcatacg ccttgcgcga
    10981 aatgcgctct tgtgcacctt gaaataattt ggtgagcggc ataggttctt tttcaagcga
    11041 aacgaagacc agtccacccc acacatccac ctgtaaaggt actaagccgt aatccttttt
    11101 atcaaaaaga tctacatagt tccaccgcgg aatgccccgc aacgatccgt cggttttata
    11161 agtccatccg tgatattggc actgcaggac tttattgcac gtaccgcgcg catcggtgac
    11221 caggggtcct ccgcgatgac ggcatacatt ataaaaaccg cgaagatggt tttccgtgtc
    11281 acgtaaaacc actaccggat tatccgctac tgtcgccgtg aggtattgac ccgcttccgc
    11341 cacctgcgaa acatggccga cgtactgcca tgatttagaa aaaataaatt cacgatccac
    11401 ggcgtgaaaa cgcgcatccg tgtaccacga cgacggtatg gtcgacgcac gttccaaagg
    11461 ttcaatcatg aattgagagg catcaaaatc tggtaacata gaaaacatcg ttttggtgaa
    11521 tgggaattat agaaatgcgg gaataaagta cacggaatca cggttagtca caacagattt
    11581 tcttcgcttc gccgcataca ttttaccctc tagaagttaa gaaacaaatc aactcttgtg
    11641 aagcggcttg atttccttta taattgacga aatctttttt cggaggttac atgaaactca
    11701 gatggtttat ttctgtcatg acggtactga tcttcgggtc gtggtacgga tttgtaagct
    11761 gccagaccaa caggcctaaa aacatcaagg tgcttgatga actgaaggac gccaccatcg
    11821 aagaaatcaa agaatccatg cgtttataca ccctttccat gggcgtcgaa tgcgactatt
    11881 gccacaataa ggatgattat gcgagcgatg aaaaaaagga aaaaaatatt tcacgcgata
    11941 tgatgcgcat ggttcggacg ctcaacgaaa gtacatttaa gaacgccaaa gaaaacgtta
    12001 cttgttatac gtgccaccgc ggcgccgtaa agttaaaaaa catcccgccc ggactgtaaa
    12061 ttccggatcc gatcatttgt caaaaagttg taaaatcgtt tgccactttt ttgatttcgc
    12121 ttgactttcc atctgtttta gataatttac tttacaagta cgctattttt tatagtaaag
    12181 actaatcatc gcatgatgcg aacattttta catacagcct tttctaaagc gttgatattg
    12241 acgtatatct tcatcggata tctcgctatc acgacgacgg ataaaccgca gtgtgtgatc
    12301 tacgcccaac atgtgatgca gaccgatccg acgaccgtcc cttcatcggt caccatcaag
    12361 gtctttgcgg cgcgtcacat acagcctgaa aaactatcac gttcgatagc ttacgtcagt
    12421 ctttttgatt tcgttatacc gccggcatgc gcaacactct tacgtgttgt taaatcaacg
    12481 cattttacgt cgtacaacgg attttccata gaacagcccg ccagagctcc gcccatcgca
    12541 taattttttc ctaaaaacag cagtacacaa aaacaatcgc ttttagagtg tgtgcattcg
    12601 tgtggccgcc ataaacgact acaccaatcg acatatttta atcggaggat attacctcat
    12661 ccgaaaagag cctttgtgtg ctgtatcaaa catttatgca accacacagg aaaaactatg
    12721 tcaaacgccg cttcaacccc caaatcaggt tatatcgccg tcggtattta tttaggtatc
    12781 ttagttctag gtgtcgtgtc tttttggata tttgctttga cacgctgaca aactcaacaa
    12841 gaaccaccta ttgcacgcga ccgtacgtca acatcttttt gacataccaa taatcgtgtg
    12901 cccattaagt cacgaaagtt ctatatgaaa acacatacac aactcacaac ggaaaccaaa
    12961 cccaaaaagg attatttatt tgtttcgctt atggctttaa tcggattgag tattcttgca
    13021 tttattttac gtttagccgg attgatctga ccaaccggta cataatctat gaatcttttt
    13081 atcaggagaa gagtgtatgt ctgaggaatc caaagtaaac cgtttttttt caaggccggt
    13141 gatgatcgga atattttcca ttttgctttt tatttcggcg cttttgtttg ttcgcgatcc
    13201 gcttttccaa caatcgtcaa acgggcaatg gttgtttttg agtattccgg tcgaatttat
    13261 tttgtttggc cttacgctgc tatgtgttgc tctgtttcac cattatgttt tacaagtggc
    13321 attgacaggg ctagccgtca ttttgactta caagttaagc tttacttcat ttgacgttgt
    13381 cgcacacatt catcacgaat gggcgattgt cgttaacttg ctcggtttgc tggtaggatt
    13441 cgccattctt gccaaacatt ttgaagagtc gcatataccg gaaatccttc cgaagcacct
    13501 tcccaatggc atggccggac cgctggtatt attgtgcatc acatttgtat tatcctcatt
    13561 tctggataat atcgcggcgg cgatgatcgg cggtacgatc gcactgacgg tttttcgcgg
    13621 taacgtgcac atcggttttc ttgccgctat cgtcgcggca agcaatgccg gcggcgcagg
    13681 cagtgttttg ggcgacacga ccacgacgat gatgtggatt gacggcgtca gcccttggga
    13741 tgtacttcat gcgtactacg gcagtatcgg tgcatttgct ttttttgccg tcgttgcgtc
    13801 aatacaacag aacaaactgc aaaccatcat tcgcgccgaa ggttcagaaa accattcgat
    13861 cgactggggt aaaatcttag tcgtagctct gatattggtc ggtgcaattg ccaccaatat
    13921 acttctggat tttcctgcgc tcggcgtctg ggctgcaatt tttattggcg cctttatccg
    13981 cgaaaccgca tggcatgaga tacccgtcgc actcaaaggc agcgtcttcc tggtttcttt
    14041 ggtactcagc gcttccatga tgcccgtgga tcaactgcca ccggcttcat ggttttcagc
    14101 gtttatactt ggattcgtgt cttccgtatt cgacaacatc ccgttgacca aacttgcatt
    14161 ggatcaaggc ggatatgatt ggggtgtttt ggcttatgcc gtcggatatg gcggatccat
    14221 gatttggttt ggttcttcgg cgggtgtggc tatttcgggt attttcccca aagctaaatc
    14281 cgtcgtgttt tggctcaaag acgggtggca tgtcgctgtc ggttatgtga ttggtttttt
    14341 catcatgctg gctttgtggg gctgggaacc gcatgaaaaa cacaaaatcg aaaaaggcga
    14401 atcgcataaa caaccgtcaa ccgaacaaac tcattagatt cctctcctcc tgattcagcg
    14461 aatagccggt gttgcactca gcgcaccggc tattcagctt tttgtagcac ccccgttaca
    14521 tgtaatttag cttgaaaacg ccgccttacc tgtttaattt gcttgtgcat ttccggttct
    14581 acacttcacc gaggagtttt atccatgaaa tctcgttttg attttttcat caccgttttg
    14641 tgcgtttgcg gcgtagtgac cctgtccgct caatccgctt ccgatttagt ccaacaggcc
    14701 aatgagaaat tttcatccgc cggttcggag aaagaataca aagaagccgc cggaatttat
    14761 gagaaagctg cggccgccga ttcgaccctg gctatcgcat gggcaggcct tgccaaatcg
    14821 cacgccatga tcggcaatat cgcttcgctg aactacaaag atgatctgaa aatcaaacac
    14881 tacaaagaat ccgccaaata cgcctccaaa gccgggaaaa tggctaaaga aaattctgat
    14941 gtgcagacgg cgctcgctat ggtgtaccgc caactcagcc aacgggataa cttgatgcgc
    15001 gtagctcaga aagcggtcgc gcttgatccc aaaaacgccg aagcttatga cctatgggct
    15061 gatgcttact ccgaagatta ttttccaaaa aacgctgata ttgattcttc cattctcatt
    15121 cgtaaaaaag ccgttgaagc caatccgaac ttcgccaaag gatatcgcgg cctcggtgat
    15181 gactacgcgt ccaaaggtga tttggaacaa gctgagtcgt actttaccaa agctatcgcg
    15241 atcaatccgc aacatgccgg ttctcatgat cgtttaggaa ggctgtatta tctacagggc
    15301 gaatttgaaa aagccaaaac cgaatttaac acggctatca aacttgatcc gcgctcgacg
    15361 tttgcctata cccatcttgc ggacgtattg gccgacgagg gaaatttcaa agaagcgttc
    15421 ctcaaattta cttatgccgt tgacctcaat cccaatgccg cttacgcaca cgccagcctt
    15481 tcatggatgt acctgacggc taaagacaaa agttttcgca atgtcggcaa agctatcgaa
    15541 tacggtgcga tggccgcaga gctcaccaat aataccaatc ctgagttttt agctgtggct
    15601 gcggaagcct attacgaagc cagttttaac aaatcggagc ttcgcgactc cgctatcaat
    15661 cgtctgaaaa cggcgttgag catagagccc gacaactttg aatatcagga aaccttggga
    15721 cggatgaatg cgaaggaaaa aagccgtgaa tatgtttacg catggaagcg cggcgagcga
    15781 ctcttaaagc ataaccgcat caatgacgcc atcaatgaat ttatcacgtc cgaagaaaag
    15841 aatgacaaat tcgtacccaa tctgatcagc tatgcgaagg cactgaagaa aaaaggcgat
    15901 gaagctacca tgaaagtata tttagaaaaa gcacgcaagg cggataagtc cggaaaatac
    15961 acgacttcga tctcgtctgt gcaataaact tatttctatg ttccaggttc agtcttaaaa
    16021 caaaaggtaa actgtatgcg cgcttgcatg aatttgtttt ttttcgccat gatgattagc
    16081 gcttcgcttt gggcgcaact caaagtagcc gtagttccgt tttacaacgg tactatgctt
    16141 tccaaaaacg aatggcttgg tcatggattt tgcgaatcgc tcaacaccga tctgagcgaa
    16201 gaaaaatctg ttctgatcat ggatcgcccc gatgtgcttg ctgaaatcaa aatgaacggt
    16261 tttaaacttc tggacatgct ggacgaacag aaagccttga cggttgcccg ttcgctggaa
    16321 gtggataaaa tcattatcgg ttcttttgac attccgggcg gcgctacacc ttcgatcaaa
    16381 gtgcaggcgc gtttcataga cgtcaaaagc ggcaaagcgg acaccaaaaa cgcttacacc
    16441 gccgaaggca aatattccat gaccaatatt tacgcgatgt acgggcaaat cgcggcgcgt
    16501 gcgcttgtta gtttcggaaa acgtgcggcg gatgaaaccg ctgtcaccgg taaaggttcg
    16561 atcaatgtgg acgcctacga accctacatc aaaggcatcc tgcagtatga cgaaagcagc
    16621 accgtcgaag attatttagc gtcgattgct cttttgcaac aagctatcaa aaccgacagc
    16681 agctttgcca tggcatacgc cggcatggca aaagcctatg ccaaaatcgg ccgcatacag
    16741 gatattaatt ttaaagctga tgaaaaaacc gagagttata aaaaggcctt agatgccggt
    16801 ctcaaagccg ccaaacttga taaaaatctt gctgtagcat ggacggctct cgctctgacg
    16861 taccgcgaac tgaaagaacg cgatcagttg atcgaagcag cccgcaaagc cgtcgcactc
    16921 aagcccagtc aatacgatgc gtacgatatg ctggccgacg cattctctgc caattttttc
    16981 cccgctcata aaaataacga ctcgtccgtt ttttatcgca aaaaaagcgt agagtacaat
    17041 aacaaatttg cctccggata ccgcggactc gggcgcgatt atttcgacaa aggggactac
    17101 aaaaacgccg aagaggcgta ccaacgcgcc atcacactca atcctaaaca tgcgggttcc
    17161 cgtgacctgc tcggtcaggt atataccata caaggccaat acgaagaagc taaacagcaa
    17221 tttcgcgaag ccataaaact cgatgccaaa tccgcttttg ctcatacaca tcttgccgac
    17281 gtactggcga tggaaaaaaa ttatgccgaa gccgtcactt cctatcagtc tgcaatagct
    17341 atcaacgcta agttcgcttt agcgtacaac ggcttggcat ggatttatat cgcggccaaa
    17401 gataaaacct tacgtattcc tgccaaagcc gtagaaaacg cgcaagccgc cgtccagcat
    17461 tcggatagca aaaacgcttc ttacctttac acgttgtccg aagcgcattt ttccgccggt
    17521 actcatgatg ccgcacttga ggctatcggc aaagctatcg ctctggatcc gcataataac
    17581 gactacgccg aaacccaaag tcgcattacc aaaaaagaaa aaaacagcga ttatgttttt
    17641 acactgcgac gcggtatgat gctgatgaaa caaggccggc aggacgaagg cgtcgtcgaa
    17701 tttgaagaag cgaacaagct cagcccccaa aacgtctaca tactttttac gttagctaaa
    17761 tattacgaca gcaaaaaaga attcaaaaaa gcgttcgaat attactatcg tgcgcgcagt
    17821 tgcgactggg acaaaaaata ttcgaaatcc attcaggaac gaatgaatga gcttgcacct
    17881 tacggaaatt agaaaacagg atatttaatt aaaaaagctg cctgaaaaca tcgggcagct
    17941 tttttaatat aatacataag aaaagaacta gatctatgca ccccaacgaa gaaaccataa
    18001 accgattcta ccgagcattt caaaataaag atttttccgt catgcaacat agctatcatg
    18061 atgacgccgt tttttacgat ccggtgtttg aagatctgac ggcggcagaa gtgcggacca
    18121 tgtgggagat gctttgtaaa cgcggaaaag atctggtctt ggaatatcgc attattcatg
    18181 ccgacgacca acgcggctcc gcggaatgga ttgcaacata tacattttca gctaccgggc
    18241 gaaaagtgat caatcgtatt cacgctgatt tcactttccg cgaaaataaa atcatgtcgc
    18301 acaaagacac gtttgatctt taccgctggg caaaacaagc gtttggtttt tccggtgttt
    18361 tattaggttg gaccggttgg atgcaaaaca aaatacgctc caccgcaaaa aacaaccttc
    18421 aacgattcat ggggaaaagt agcccggttt cttagaaata ttacttcttc acatcggggt
    18481 attcgtgcac acccttacct gttttgcgac caagtcgtcc tgctttgaca tactgcacca
    18541 tcaaagggca aggtttgtat ttctcaccga gcgatttatg caaatactct aaaatcgaaa
    18601 gccgcgtatc caaaccgact aaatcaacca tttcaaaagg tcccatcgga tggttgagcc
    18661 cgagctttaa tgcggtatca atatctttgg ctgaaccgat accttcctgc agcatataaa
    18721 acgcctcatt gccgatcagt gcattgatac gactggtaat aaatccaggc gactctttga
    18781 tcgttacgat ttctttaccc atcgctattg caacatcttg cgtagccttt atggtggcct
    18841 catccgtttc caatgcttta atgatttcga ccagtttcat tttgtgtacc ggattaaaaa
    18901 aatgcatccc gatacacttt tgcgggcgtt gtgtcacggc cgctatttca gtaatgctca
    18961 gcgaagacgt attcgttgca aatactgttt ccggtttgca ggtcttgtcc agcgtcgtat
    19021 aaattttgat ttttaaatca attttctccg gaaccgcttc aataacgaaa tccgacggcg
    19081 cgacggcggc ttcaagatgc tgttcgacgg atatacgact gagcgtcgcc tctttatcct
    19141 gcgccgagat ttttccaagt tcgatacctt tatccaggtt tttggaaatc gtattcagcg
    19201 ctttattcaa tgcgtccagc gacacatcat gtaaaatggt tttatatccc gcttgcgcgg
    19261 cgacatgcgc aatgccgttg cccataatac cggatccgag tacagtgatc gtttgaacag
    19321 ccatcgttta taatctcctc tgtaaaatgt tttttaagtg gtgcaataat agaacagctt
    19381 tgcaacggaa gcaaataaaa tatcttggat attgactgtc ataccggcac actacatctg
    19441 catctcatta ttatcccgca tgacttccga cataccccgg cgatgcggca cgtgatatat
    19501 tatctcgtga taaggcgttt tttatgatag catttcaatg cctatttata tattttgccg
    19561 cggcttaaaa aggagttttc acagtgacaa tcgaacctta ttttacgaca ggtcaacaca
    19621 atcgtttttc agaaatggcc aaaggccttc tcggatcgga gattctcaaa atcgcttccg
    19681 acattcgcgc tatggtggcc aaagggcaaa aaatcggaaa tctcaccgtc ggcgatttca
    19741 gtccggcgga atttcgtatt cctcagttta tggaagacgc cattcgccgg cattatgccc
    19801 aaggacaaac caactatcct ccttcggacg gcatgctcga gctccgcaac gcaatcgtcg
    19861 gcttttataa aacatggctt gatgtagaat atccggttaa gtccgttctc gttgccagcg
    19921 gtgcacgacc attgatctac agccttttcc gtgcgatcgt ggacccgggc gacaaagtcg
    19981 tttaccctgt tccgacatgg aataacaata atttcgtcca aatgctcggt gcgcagggcg
    20041 ttccggtaca ctgtcatgag gaacatgcgt ttttaccgac ggtggattta ctgaaaccgc
    20101 atttgcacga tgcacgcctg atatgtctca attcacctct caatccgtcc ggcacggcgt
    20161 tttcacgtca ggaacttgag gccatctgcg acatgatcct tgaagaaaat aaacgccgca
    20221 ctaccgaaga cgctttgtat ctgatgtatg atcaggtgta ttggatgttg actttcggta
    20281 aaacagagca cttcaacccg gtacaccttc gcccggagat cgctccctac acggtgatgg
    20341 tggatgcggt ttccaaagcg tttgccgcta ccggtgtgcg tgtcggatgg tgcgtcggtc
    20401 ctccggatat catcgaacgt atgtccgcca tactcggcca cgtcggcgcg tgggcaccgc
    20461 gtgcggaaca aatggccacc gccgaacttc tcaacaataa agatgaaatc gtcaaatacc
    20521 acagtgtcat gaaagtaggt gtgcaggatc ggctcaacgc tttatacgaa ggtctgtccg
    20581 caatggccaa ggacggttta cccatcaaaa cgatcgtacc catgggcgca atttatcttt
    20641 cggtacaatt caattttctt ggccgcaaaa aaccggacgg aaccgtattc accaatagcg
    20701 aagaaatccg ccagtacctt ttattcgaat cacaaatcgg cgtagtaccg tttaaagctt
    20761 tcggatatca gcatgatacg ggatggtttc gcttatcggt tggcgccgtt tccatgaaag
    20821 atatcgaaga ggcgttgccg cgattgcgtc gtatcttaga aaaattagtc tgaccggttt
    20881 atggataaaa acagtatgac catcaaaccc ggtcttgcgg cgcccgaatt gcgcatttcc
    20941 gaatggatac agggtacacc aatccaactt tccgaattgc aagggcgcgt cgtaatcatc
    21001 gaagtatttc aggtcaattg cccgggctgc tttctctatg gtttacccga aattttggaa
    21061 atcgccgcca aacataaaaa cgaaccgctt accgtgatcg gtctggcaac ggcgtttgag
    21121 gattttgaca tcaataccaa agacaatctt caaaaacttt tacaaaccgg tgaattggtc
    21181 ggtgaaacgg cacgcgtctt gaaggaacat gactgggcaa ccgacaacaa actgcgttac
    21241 acgattccca ttcccgtcgc aatggatcgc ctgggtgaag gttcacgctt ggatacggtt
    21301 tcacaaatcg aacgcatcat cgaacgcgat atcaccaact tcgatcgcct cagctacggg
    21361 gaacaaggac gtatccgcga atccatattg aaatacttaa acgaacgcac acttgttccc
    21421 gaaacatttg atctctacaa tctcagcggc actccctcca gtattgtcat tgataaaaaa
    21481 ggcatcctcc gtcatatcca gttcggctcg acaggaaatc tggaaaaaat cattcggccg
    21541 ttgctgaccg aataaaaatc tcacattata aaatcttctt ctaataacag atcaaatttt
    21601 aaaatagaga atcaattctg tcaaaaactc attggaataa aacaatgaaa accattgaag
    21661 aagcatttat ccatgaagga tacttaagta ttcctttttc ttacaatggc gccggacacc
    21721 ctattatcaa agttactgtt gcgaatggtt ttcaaaccaa cattctgctt gatacgggcg
    21781 cctctgctaa tttacttgac ttagaattcg cacaagaaaa cggactgaat ctgacaccga
    21841 ccggtgaaaa aggcggcggt gccggcggac tcacgctgga tgtattcacc gttgacaaac
    21901 ttacactaga aatcaacggc aaaaaacttc aatttaccga ttttctttcg atggatttta
    21961 cgacaataaa acaatcattg ataactaacg gtttagaagc tgattttcag ggtattcttg
    22021 gttttggatt ttttaagaaa actaaatgtt ttatagatta ctcttcagat agaattttta
    22081 ttcagaacaa ttcttatatc gaatgatata gccttcgatt taccgttttt tttcgatata
    22141 cttcgactga ttgaattcct tgaagctaaa catatcatga tgtttgtagg ctatatcatc
    22201 aaacatatca ccccatacat ttacccatga cttcacttta caggcctctt ggtaacacag
    22261 gatatacttg ccatcctttg ggtttcggca gttatcggat aaacgaggac aacccgacgc
    22321 acgaacgtgc cttgcgttcg tatatccagc acggcggcaa tcttattgat accagcgcca
    22381 attatggcga tggcgcttcg gagacattga tcggaaaaat tctcagcgat atcaatcgca
    22441 gcaaaatgat cgtggtttcc aaaggcggat acattcaagg gcaaaacctt gccttagcgc
    22501 aaaagcatga ttttccggaa gtcatcaaag tgcaggataa tctctggcac tgcattcatc
    22561 ctgagttttt ggaaacacag attacccgct ctctccaacg ccttcaaagt gattacattg
    22621 acatctatct cctgcacaac ccggaatatt ttatcaatca tcaatccaat ttcggtccgc
    22681 ttgacgatac ggtacatgac gcgttttatc gtcgcgtgcg cagagctttc gaatacctgg
    22741 agtcgcaggt tgccctgggg cgtatccgat actacggcat cagctccaat aatttcggtt
    22801 atcatgcgca agatcgcgca cgcacggacg tatcgcgctg ctgggcgatg gcagaagaag
    22861 tttcacccca tcatcatttt cgtgtggtac aaatgccgat gaacccgtat gaagcaggag
    22921 gcgccatcta tcccacgcaa aacggtttga cgccgttgga attttgcatc aaacacaaca
    22981 tcggtgtttt aatcaatcgt cctctgaacg cttttcatga taaccgactt attcgaatcg
    23041 ccgactatgt gacacccgga caaaataaac cggatatttc cacgcttcgc gacgaaatgc
    23101 agcccctgat taatcttgaa aacgaattta aactgcgctt caacggtagc ccgttcggcg
    23161 atgaagagca gggccttgtt tcttatttgc tgtatatcgc cgatgatctt ccatccaaag
    23221 aacattggaa tacggtgata gaccgctaca tcgtaccgcc ggttacccaa tggctccgcg
    23281 atcaaagtgc actctacagc gatagtatcg tttggaatgc ttggcaggaa aattttgtca
    23341 aagccgtcaa tctcacattg gatcgcgtcg aaaagtatgt cgcctacagc gatcagaaag
    23401 tttccgatcg cgtccgtcaa cggctcatgg actccggtta tccgtcaagt gatgcgtcgt
    23461 tgagccaaat ggcattgtca ctgctcatac aaacgcaagg cgtctcttgt gtactcaacg
    23521 ggatgcgcac cgaatcgtac gtcaaagatg ctatgggcgc actggatttg cctgcggtgg
    23581 atgccaaaca tattttagaa cgctttgatc aatccattcg ctaaatgaaa acgctcaagc
    23641 tcaagcctac ggatgcaaat atccgtaaag ccgcgcgcat cctacagaaa ggcggtttgg
    23701 tcgcactgcc cacggagacg gtgtatggtc tcgcagccaa tgcgatgaac accaaggctg
    23761 ttcggcgaat tttcaaagcg aaaggaagac cctctgataa tccattgatc gtacacatcg
    23821 ccgatcgccg gatgctcagt actttggtgg acacgatccc ggaacacgcg cggttactca
    23881 tgcgtcattt ttggccagga ccgcttacac ttgtacttcg acacaatggt acactaccct
    23941 tgaccgtaac tgccggttta tccacggtcg ctgtacgttt accgggactt gctttgacac
    24001 gaaagatcat tcgcttatcc ggcgtacccc tcgccgcgcc gtcggccaat cgctccgggc
    24061 gaccaagccc tacgcaatcc gaacatgtct tgcatgatct tgacggacgc attgaggccg
    24121 tcatcgaagg gggcattgcc cgcgtcggta tcgaatctac agtcgtagat gtcaccggaa
    24181 aaattccggt catacttcgc ccgggccaca tcacgattga ggatatacgt cgtgtgtgcg
    24241 atcatgcccg tttgtcacac caccgtcggc ataccagcgt acgttcaccg ggtatgaagt
    24301 accgtcatta tgcaccggat acacctctac aaatcatcac acccgaaaaa atcgaagcct
    24361 atgctaaacg caacacgcat cttcgacttg gtatcatgac catgcgtcgc ggtttgcata
    24421 tacccaatgc cactgtgatt tatatcggca acacaccgac acgcgctgcg cggcatgttt
    24481 tcgctgcatt gcgaaaatta gacggaaaag tagatcgtat tgtgtgtgaa gcattggaag
    24541 aaaaagggat cggccatgcc gtgatgaatc gcctgaaaaa agcagcgagt aaaaaataaa
    24601 ttatacgacg gagataaatc atgcatcggg aagcagcttg ggagcttttg tgcgagtaca
    24661 ccaaaggcga aagccttcgt aaacatgctt tagccgtaga atgtgcagtt cgggcttatg
    24721 ctgtaaaatt tggcgaagat gttgagttgt ggagtgtcac cgcgctattg catgattttg
    24781 attatgagat gttcccggat ccggttgcac caaacggaca tccctacaaa ggaaacgaaa
    24841 tactcaaaga caaaggttac ccggacgacg tgcgtcgtgc gatcatgtcc cacgcggatt
    24901 ataccggtgt ttcgcgcgac tctttgcttg aaaaaactct ctttgcttgt gatgaattgg
    24961 ccggtttcat aaccgccgtt gcgtatgttc gtcccacaaa gtcggtaaaa gaagtcgaag
    25021 taaaatccgt tcgaaagaag atgaaagaca aggccttcgc ggccaaagtc aatcgcgatg
    25081 atattatcaa cggagcggcc gcgctcggca tagatctgga tgaacatatc gcttttgtta
    25141 ttaaagccct gcaagataat gcggattcgt tgggattata gcggatgtat taaacggggt
    25201 gatgaccctt tggtaaaaca atgcgcacgg atgtgccttt tccaacctcc gatgacacat
    25261 caatatgtcc gccgtgtttg tgaacgatac cgtatgcctc cgataaacct aatcccctcc
    25321 cctgacctac cgggcgcgtc gtaaaaaacg ggtcaaatat tttcttaaga tcatcgggtg
    25381 caataccgac accattatcc ttgatgacga tagatatcca cgcctgatcg tgcgccaaag
    25441 ctgatgccgt ttgtacgcaa ataataccgg aaccagagcg aatcaccgcc tgcgtttcag
    25501 cttcctgtat cgcctgcact gcattggtca atacattaaa aaacacctga ttcatttccg
    25561 atgcgttaac ataaacaata caaccttcgc ccagtgaggt atcaatgcga atgtcgttgt
    25621 gctgtttgac aaacaaatca atgatgcgga gtaaatccgc attgatatcc gtttctttgt
    25681 aaccgctttc accgatgcgg gcaaactttc gcaggttttc gacgatatca cggatgcggc
    25741 gcgaaccggt aataccattg tttatttgct ccaatagttc atgcatccat ccgtgcgttt
    25801 cggacggaga aggaagcgct gtggattcga tcgtagcact accttcagca taccgacgac
    25861 gcattttttc aacactgtct ttcaaccctt caaagttacc gtaaacgaag gttaacggat
    25921 tattgatttc gtgtgcgatg ccggctgtca tctgaccgag cgaagccatt ttttcagatt
    25981 gtacgagttg cgcctgcgtg tcccgaagcc gcacgatcgt atcatttagc tcttgattga
    26041 tcttggccag ttcacgtgcc ttttccagtt catgacgttt acgttcattt tgaatgcgta
    26101 tgcgaacaaa aagcattagg cctaacacaa ccaaacccgt ataaaccgca taagcccacc
    26161 atgtttgcca ccaaggtggt gtgatacgaa tagtcagaac gcatgtgttt tcgttccata
    26221 tcccgtcgtg gttagttgca cgcacttgga atcggtacgt accgggatcc agattggtgt
    26281 acgttgcgat acgacgcccg gcatcaatat aattccaatc ttcgtcaaag ccttccattt
    26341 tataagcgta cctgtttttt tcaggttgcg taaatgtcaa tgcagagaat tttaatccga
    26401 ttaccgattc gcgataggat aagacgagct cttccaattc ggatatcgct ttgggaagta
    26461 taaatccgtt atacgatttt cccacttcta ccgtttgatt gaataactcc aggccggtga
    26521 tcacgacggc aaatttatcc gtattatctt tgatgctgtc cggatgaaaa taatccaacc
    26581 cattaattcc tccgaagtaa aaccatccgt cgtgcgaacg aaaatacgca ttctgattgt
    26641 attcattatt agctaaacca tcggtcatat catagttacg aaatgactcc gattgaggat
    26701 caaatcgtgc gataccgtaa ttggtactta gccaaagacg acgttgttta tcttcaagga
    26761 tcccgtatat aacatcactg atcaaacctt gtcggtgatt gtatagtcgc agcgtagacg
    26821 aatccggatc ccatctttgc aaaccgacat ctgtaccgac ccacaaaaca ccgtcatgat
    26881 cttcatagat cgaacgaaca cgatttcgtg agagactgga ggatcgcggt gtgtttgtcg
    26941 cgaaatccaa atctataaac cggcgctctg tgggaacaaa tgattttaat ccgctggcag
    27001 tgccaatcca aagtttgcca tcacggcctt tatgtaatgc cagtacaggg tcattggaaa
    27061 aataaccggg ctcacccgag gccggttgaa ctctctcaaa atttttcttc ttttcatcaa
    27121 aacgacaaaa cccgcctccg gtcgtaccaa accacatagt accttgtgga tcctcagcga
    27181 tggaccaaac gccgttattc ggtaaagagt tttctgacga ggggtcatgt ttgtaaatcg
    27241 aaatcttatt tgtcaatgag tccatgacag ccaatcccgc tgcgcttgtc cctatccaca
    27301 aacgcccttt ggtgtcacgg cgaatgatac ggatacggtt ttccggtaat gatgatgatg
    27361 cgcccgattc cgacgtaaaa aaagaaatat atcctgtgcg cgaatccaaa cgatttaacc
    27421 ctttgaatgt tccgagccac atcgttccat ccgtatcttt ccaaatacta cgaacacggt
    27481 tatccgaaat ggatgccaaa ttatgagggt catgacgcat cgaacgaaaa tcttttttcc
    27541 gtgtgtcaaa aacattcact ccgccgctat atgttcctat ccacatacgc cctgagcgat
    27601 cacaaaaaaa cgcatagagg ctattgtcac ctaaacttgt agcatccttc gcatcatatt
    27661 tgtacgaact cacaatacgg cccgtacgat caatcgtgcg aataccgctg ccaaatgttc
    27721 cgacccatac cgtttcatcg gtattttctg ttaatgcaaa aacggctctg gatgtcaaga
    27781 ccgaatcctg cacggatcga aagcgtcctt gctcctgatc gctacgatca agaatactca
    27841 aaccgttata cgtaccaatc cacaacgtac catgttgatc ttctataata gtgcgtatgc
    27901 gatcatccgc caatgatgac gcattgcggg gattatggcg atacacctgc atctgcatgg
    27961 tttgcggatc catacgatac aatccaccgc caaaagtacc gacccatagt tttccatcct
    28021 gcgtcgcacg aagactcatg atttcattgg atgcaagtga agtattgctc gtatcataaa
    28081 tcgtgaaagt atgcgtagtt tgattaagcg tcgctagacc accgccaatc gttccgaccc
    28141 ataatgtgcc gtgaacatct tcggagatgg cccatacacg accgtgcgga agcgaaccgg
    28201 gtttgccttt tcgcgccgaa taccggacaa atgattgggt ttgcctgtca taacagttaa
    28261 gcccgtcgcc atccgtacct atccataatt ttccgtttcg atcctcatag attgtttgaa
    28321 tccagttatc tgaaatcgaa agactgtctt gcggatcatg acgaaatatt ttaaaaccgt
    28381 atccatcata acgattaagc ccatcttggg tacccaacca tatgaacccc agtttatctt
    28441 gcaaaagaca atggacggcg ctttgcgata aaccttcttc gatggaaata cgcttgaacg
    28501 aggcctgttg actaaaaaca gaaacggata aacccgccaa accgaacatc attcctgcaa
    28561 tggcagaaaa gaaaacaaaa actaaactcc gttttttgga agcagagacc atcaataaaa
    28621 cccgtatgag taaaaggaaa tactggtgat taaatgaaaa ccgaaagcca aggaggctta
    28681 acttaattca aaccaataag tatcgctact acattttgca tatagttttt ttaaatcact
    28741 ttgagggtaa tgccttatca cggcatgcgc acggaggaga gaacctatta tctatgcgtc
    28801 ttcttccgga tacaattttt gtacacactc gggacagatg ccatgcgtca acatagcttt
    28861 tgtgttcgct tcgatataat actctaactg attccaatag cctttatcat cacgaatttt
    28921 tttacacgat gcacatatcg gcagaagccc gcttagcgtt ttaacttcgc ctaaggccgc
    28981 ttgcaattcg gcaatcagct tttctctttc tttttcatga cgcttacgtt ccatgatatc
    29041 acgcgcaaac gtgatgatat actttttatc ttcatgcgta aaaacagata ccgatgcttc
    29101 gacatctacg aagtcgccgt tcttattacg tatctggaaa tcatacaacg aaggcacttc
    29161 ttcgcccaac gaacgacggc gaccatactc caaaagcctc ggcagatcat gcggcgcggc
    29221 caattccgaa aggtgtaaac ccatcaattc atccgcgctt gttcgcccaa acaattttgc
    29281 ataggccgta ttcataaaaa caactttttc gttttcttct actaaaactc cgtctcgtga
    29341 agtttctaat attttacgaa tgtacatctc acggcgacgc gtttcttcgt cggccatcgt
    29401 tttttcatct atctgcgatt gcatattcag aaggtgccgg cgtaattcca attgatcttc
    29461 tatctgatgc gccaatgcac gtaaagtctc tttttgttca tcgctcagat cgcgcggctt
    29521 attatctaaa acgcatagtg aacccagcgc aaaaccgtca aacgaaaaaa acggcatgcc
    29581 ggcataaaac cgaagttgcg ttgcaccggt aaccaacgga tgatgttcga aacgtttatc
    29641 ttcggaagca tcgcgaacga taagcagttc gtcgccaaga atcgtgtgcg aacaaaacgc
    29701 tacatcacgc ggtaggtgta ccgtcggaaa acctaccgat gatttgaccc actggcgatc
    29761 gctgtctatc aatgtgatca tcgcgaccgg cgtcccgcag atatgggaag ccaaacgtgc
    29821 caaatcatca aaacgttgtt ccgactccgt atccaagata tgatacgaac ggagacgttt
    29881 tacgcgtgcg ttatcatggg aggtctctgt ggatttcatc gtagcaatat acgatgttaa
    29941 tgtattgaga gggaagaaat ctttttatca ctccagttcc accatttcaa tttttccggt
    30001 tccggatctg ggtgcaatgc ataataaacg gcgcagcgaa aaacatataa catacagcga
    30061 tctacgatct gaccacgccg tgtacaaaaa tcctcataca gtttttctgg attttttttg
    30121 gctaattcgc ctaccgaacg aaaacccata tcccaaaagt cttgggctat acttttgccc
    30181 acccctggga tttgcataaa ttcgcgtatt actgatattc tgttcgtacg gtgtcgtgtt
    30241 ttcataaccg atcaaaattg catcgaacac ccgatcatat aactccgccc gggcatacga
    30301 taccgctcga tctcttcgta ttgcgtattc gtaatgtttt gcgctgaaat atacacactg
    30361 cgtttgggta aaaattctac ggagaatttg ccattcaaaa caaaaaaagc gtccggttcg
    30421 ctcccgggat aaatggaaac ttcacgaatg gcactgttat aacgaccgtt gaggttaaga
    30481 ctgaccggct cgtagccagc gtctatgtta aaattgaatg aatgtttggc tttgtacggc
    30541 agatcatgat tgccggaagt ataaccaaca tcgcgggttc ccgcatcttt ggatatgtcc
    30601 cgcgcatcaa gtaatgtata tcccgccgaa gcattgaggt actgtttgta aaaaacgtgg
    30661 atgctcgctt cgacgccgcg catgcggg
//
LOCUS       DAOQNQ010000009        30188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_484_length_30188_cov_5.793449, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000009 DAOQNQ010000000
VERSION     DAOQNQ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 30188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30188
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_484_length_30188_cov_5.793449"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PK754_01275"
     CDS             <1..547
                     /locus_tag="PK754_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB08166.1"
                     /translation="NIIGLLDNPTSGEYHFVGHEVSRYSERQRSNLRKANIGFVFQSF
                     NLIDELTVYENVELPLLYLGMTASDRKKRVEETLEHMQIMHRRNHFPQQLSGGQQQRV
                     AVSRAVVAKPKLILADEPTGNLDSAHGEEVMRLLTDLNKAGTTIIMVTHSPSHAEYAH
                     RIIHLFDGHIVTENMKEAAKI"
     gene            601..2973
                     /locus_tag="PK754_01280"
     CDS             601..2973
                     /locus_tag="PK754_01280"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB08167.1"
                     /translation="MIRNYLKIAVRNMQKNALHTFFNVLGLAMGIACCITVYIFVDTM
                     LNMDTAHREAERIFTINHVRLINNREEFWGLSKGPLGPAIKNDFPEVTDFIRLDDNNA
                     VVKRGDLIFNEDIRFADANFFSMFTFSLASGVGSDLAQKNAIFLSEEKALKYFGDQNP
                     VGQTLTLIFEGEKRLEFVVKGVAKKFPRNTSFAFNFLVNYENIKDIYPQADRWTYNVS
                     ATFIKLHRPEDAFKVEQGLSRYLALQNESNPNQPAQRFYLDNLLTMSKNSWQVRGDIS
                     GGTNPHGIYTLTFIAALVMLMACFNYINIAVASAARRFKEIGIRKVVGGTRGQLVKQF
                     LTENFITTLTALGLGVVFAETLTIPFFNSIIQGNTVLAFDISADLRVVIFLAVLTSSI
                     GLLAGLYPSLYMSSFKPVNILKDVQKIDGNRWLGKTLLVLQFSISLFAVIAGIIFTQN
                     AEYIKTLDYGYEDDQIVALYFKQSAQFHQFEQKIRQDSRIHAIAGSAHHAGRNIMGNV
                     VYESNGAPSQANVMRVGSDYIQTLALRMKQGRAFDRAMTTDAENSAIVNATLVKAQGW
                     TEPIGQRLKMNDKELTVVGVVEDFFYRSYHRAIDPCILTMIPDDEYRYMSIKVAPEHV
                     LSIRPLLESTWREIMPDEPFDMIYQNEIFLEDLTEDEQVKNVFLYVAIMTLMIAAMGL
                     FALVSLSIAKRTKEIGIRKVLGASVAHVIHLVNRDFYWLIGIASVIIFPIAFFLLTNL
                     LNSIYAVHVDITALPFILSVLIFGLVAIITIGMRVYGVAKENPVHALKYE"
     gene            3053..5455
                     /locus_tag="PK754_01285"
     CDS             3053..5455
                     /locus_tag="PK754_01285"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB08168.1"
                     /translation="MIKNYLQTAWRVLLRNRGYASINIFGLTLGMTCAFMIFLIVRFH
                     LSFNDQHRFKDRLYRVTTTDKRAAQEDETSMGTPYPLSGLLRTGFPEIENVAVTDYVR
                     NGQVLISQQNGEPKKFSETGGIAFVQPQFFDMFDFPWIEGTPKTLDELNTVALSQTLS
                     QKYFPEGNPIGKIIRLDNEHDLKVAGIFKDFPANSDFPFQMAIAFETSRAMYPNNKTE
                     WGSTSSNTNTYVLFKENFDLRAAEAKLNEEAKKHREIRPGFDRIFLFQPLDAIHYDSN
                     FGSYAGATISQNTIITLGLIGFFLIVTACINFVNLATAQAVKRAKEVGVRKVLGANRF
                     QLVAQFYGETILITLFAVILTFGLTEFSFPKVMEWLDLTIPSVLIWNADSLLFIAALS
                     VMVIFLAGLYPALVLSGFGPALALKSKITSYHSGGLYLRKGLVVLQFSISQLLIIGTI
                     IATTQLDFFNKQDLGFNKEAVVIVPIHERTKTLLDNLEHQWKSHPGVESVSFAYASAA
                     SGNRWTSNVTFLKKDEPIELDVDLKMADDHYADIFGLQFLAGRNYVAGDTLREVVINE
                     TMVRKLGFSNPQDAIGIELKIGARRRVSVVGAVRDFNASSLHDPIAPCIIATNSRFYQ
                     EASIKINMQQATDVLRHIETVWNTAFPQYVFDRRFQDETIAEFYEQEQIYTNMLRIFS
                     GIAIFIGCLGLFGLVSFMAAQKTKEIGVRKVLGASVTDILGMFAKEFIALIILAFIIA
                     APLAYYAMQQWLQDFEYRITLGPAVFAAAVMVSLIIAGATVGYQVFRAARANPADALK
                     YE"
     gene            5488..7857
                     /locus_tag="PK754_01290"
     CDS             5488..7857
                     /locus_tag="PK754_01290"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB08169.1"
                     /translation="MIRNYFIIAFRNLYRKKMYTFINLFGLSVSIAVCIVGYLIWDYS
                     YHFDQFHENAERIYHIGTTRVINEQDQIFGVSPLPLAPALARDFSGVKRAVRFANQVA
                     IVRVGDKVFNESIHFADEQFFEMFRFELEHGRYDALKSKTDLIITHDMAMKYFGEANV
                     VGQSMTISYANEVKQTYTIGGVLKTLPRNSSLRFGFLLSSEILSDLGVDERNNWSHVT
                     GATFIEVHQVETIQQIKGSESKYIELVRSANPRFPISHLVITPLKDLALEGESFRSHD
                     LQDAPPPSARISLFVTSILLLVMSCFNYMNTSLAMATARLKEIGIRKVMGSPRTQLIF
                     QFLTENVLLCVLAIFAAFGLAEFLVPGWNALFYDIQLSMDESKSPGLWLFLIGLVGLT
                     AVGGGLYPAYVISAHNPAKILQGHQSKTTASWIMKVMLTLQFSVSIIAVVGSVVFSQN
                     AEYQKNLDLGYEPEKLIGVVIQKSSMYPVLSNALMQNSQIESVSAGRNHILYSSWRRS
                     IKVVDRDIEAEFLYVRPGYTETVGLKLAEGRFFDVSQPLDLTESIVINRMMAKEQGWE
                     NPIGQTMVIDSVTCRVVGVIEDFYIRGPFRPILPCVFKAANEDSYRFVIVKADHNNIH
                     AAMRSVEETWKKLFPEMPFDGFYQETQTANAAELSKSIMLVFFCVALMAIIISGMGLF
                     ALVSLNIVRRTKEIGIRKVLGASALQIMRMMNSDFIILLLIASVIADVAGFFLFKALL
                     GSIYRYHVEVGIMALILANIAVLLIGLATIGGRVLKTARANPVNALKYE"
     gene            7887..10301
                     /locus_tag="PK754_01295"
     CDS             7887..10301
                     /locus_tag="PK754_01295"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB08170.1"
                     /translation="MSRNYLLIAIRNLFKQKGFALINTSGLAVGIAGCLLILLFVRDE
                     LSYDQYHSKARRIFRLQTNVDVSGNLLTLATTSYPMAEALKTDHSQVESAIRVMRWND
                     PILSQGDIRLKETSMLWADAGVFDVFSWPMIYGDPKTALQEINSVVLSESASKKYFGD
                     DNPIGKILRYDGRIDLLVKGVIKDIPYNSHVRFEVLANIQTLLDVVGRESMSHWHAFY
                     QLQTYLLLRNTFDAQSLIESLPSFVDKYLKGEFAESVGRTYTMELTPLTELHLSTSRK
                     GEFSTVGSMNYVYVFSGIAFCLLSIACINFINLSTAHATKRHREIGLRKVLGANRSQL
                     IRQFLTETTLLVFISITVALLLIDIVLPYFNTLAGKHFTFSIFADYTTLLTIPGLVVI
                     VGILAGMYPAFYLSRFNPIESVRGSHYSGTKKTRFRHALVSIQFVVSIVLLIITAVVF
                     RQLDFIQNKNIGIDKNQTLVIPVTDQQIRARYHSLREQLTTQPGIENVAISGFMPGIR
                     VPGSPLQKIPTGPSDKWEFSTIPADENFVPTLGINIIAGRNFTKLNPTDSAESMLINE
                     AAARDLGWTPEEAIGKPLAWGGDPGFNVRVIGVIKDFNYTSLHQPIKPLVIIPTALWP
                     NSYNFLSIRINTGHLSETIHHINTVWEQRMPETPFIYSFLDDDFSKLYAGENKLGIIF
                     LIFASLAIFVASLGLLGLATFVSESRRKEISIRKVVGADLRTLIILLLGDFLMPITIS
                     TLISWPIAYYIASAWLQNFAYRTEPTWYVFVAATLFSIGIAALMLIQYVIKTARVNPA
                     VTLKSE"
     gene            10415..12808
                     /locus_tag="PK754_01300"
     CDS             10415..12808
                     /locus_tag="PK754_01300"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB08171.1"
                     /translation="MVKNFLIVAMRTMRKHKSYTIMNVTGLATGLACFMVIAMYVRRE
                     TAFDTFHKNPDRLFRVALYRENTGSPQPIATVSHSVADILRRESPGIESMARLFRFDQ
                     DAVILAGEKKFTEPDLMFAEPDVFDLLSIELLNGSPANALKNPTDAVLSEASARAYFG
                     HTDVIGQSIRVRINTRYADFKITGVFKNYPSNSHFYTNVLLGFDGVKNYVGDVDFFQS
                     WFNYPMWTYLRIKDIQDEVSVNAALHQIVERHFPQTRKPAHMQLQRITDIHLHSQMSG
                     EIRPNSNIFYIYTFTVIGLLLLIVACVNYINLSTAQSEQRTREVGVRKVMGARRRELI
                     FQFLMESVLQSMLALGLALGLAEVLASVLNRFTDLQLTFDYSDPFLFIFSAFLIVVVG
                     VGAGFYPAVVLSGYAPIRNLRMKAFRRRFPLRKILVIVQLSVTAALISAVWIIQLQIN
                     YVRSKELGFNKDHTVLIRAIGSTVASNLNYDRFKYQCEQLPGVISVTRHSWVAGEGFR
                     IRSVGFDSFSEEQRQPIQFIFVGEDYLKTYDLKLIEGRDFSAQYPADTAGAYIVNEAA
                     VKAYGLTNPIGRIAVSGDRGPVRGPIVGVVKDFHFASLHETIEPMVIGLFRFPMPYIS
                     VRFDGQATPETIGAIERLWNNIDTERPMDYSFLDTRIERVYRFENQLSAITKLFTILS
                     MIIGCMGLFGLIAAMAEKRTKEIGIRKVLGASIWGIIGMFAREFSAMVIVATLLAVPT
                     VYSGLSQWLEGFAYRVELTAEPFIVSFIAILFLTVITVAHHALKAAKTNPAVSLKSE"
     gene            12897..15275
                     /locus_tag="PK754_01305"
     CDS             12897..15275
                     /locus_tag="PK754_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB08172.1"
                     /translation="MFKVYLKIILRNFLKERSFVWINLIGLSVGLLCCFTILLYARKE
                     LAYENFHVNKDHIYRVLPRIQGGANDYEQIWTPAWLAPSAKNKIPEIRLASRYIHLGS
                     EQLITHENQRMLTDGLALADPDFLHMFSFTEPFTSDMNPLSDPSALIVSRSAAYRIFG
                     DQSPIGKTITLNGSHVFTIKAVFTDVPVESHLQFEMLGNFQRIREIWGMNTTPEENTS
                     WNFATYLMTTPQADISAVQSKVLNIFEERAPRKNQRTVRLQSLNDIHFTQGIRRDNAD
                     GNWTSLIALCSVAVLVVLIACFNFTNLMTAKAMRRAKEIPVRKISGASRWLIALQFIS
                     EALMLSVFATVLAIGMLEGLLPLLRHYLDISIPFTYTEHGHWLGIMIGVGILTGLIAG
                     FYPAWVLSSFEPARTLKGVAKLPVANKLRKGLVLSQFTVSIILASCTVIVWQQINHMK
                     EFNLGFQKEQIIVTSAPGSLREKPETVRQQLTNDGSIISVTFANSVPGNAFSHGPYEI
                     LGGTENGRISINTIYADAEYLKTLNIALSEGRYFYPEHAAENTNYYVVNEAFMKAYGK
                     SDFQTMRLRSLDGNHDKPGLIVGIVKDFHYASLKSAIAPLVLRYAPAESWRMIIRVAP
                     QRTKQAITKLSSVWQTMAPEIPMTYRFLDEVFDKQYKQEDRLHHLMIGFSALAILIAG
                     LGLYSMVAYYNEQRTKEIGIRKVLGATITDVLYLMSRDYVGMVILANIISFPLIYFMM
                     NHWLDDFAYRVTVRWEIMAITGILTLGLTLIMVGIQSWRTASANPIHALKYE"
     gene            15309..17663
                     /locus_tag="PK754_01310"
     CDS             15309..17663
                     /locus_tag="PK754_01310"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB08173.1"
                     /translation="MLKSYLTMALRTLSKSKAYSFINIAGLAIGLTCTTLILLWVQDE
                     LSYNRFHEKLPRLYRVMESQHYANGEIFTTSATPAPLLYHLKERFPEFEGISRMNFWN
                     NILIEYGEKRMYENDGLGVDPDFLTMFSFPLIKGDPLTALNQPDAVVLSETLAKKYFG
                     NEDPIGKTIKMGGRDLFKITAIAADPPLQSSIKFNLLWSLSYLKETQPDKSNWDNNWL
                     RLFAVLQPNQKGTDVSEKIKNVIIDRNPNMAVRIELFLQPLSDMYLYSDFNKIPPRIN
                     YVYILSGIAFLILLIACINFTNLATARSIKRAKEVGLRKVVGADRFQLIRQFLIESVT
                     ITLVAMMLSLILIELLIPLFNDLSGKQLSLRWDDIQTFSLMCAMVLFTGLVSGLYPAM
                     VLSRYQPALVLKGSFSKSIQGMRLRKILVSLQFTLSIALVICTIIAYQQLQYMTNKNL
                     GYDKEHLVYIRLLDDTAKQYPVLKEKLQQKPGIVSVSAADQTMTGFGSNTWGLKWRGQ
                     QEGQRILTTFLRVDYDFFETHGIQVSEGRFFSKNFAADSGAFIVNEAAIRQMKLENPL
                     QETITLWNTQGPIVGVVKDFHFQSVHQEITPLIFILKPERVNVMTVRIGPSETTKTLA
                     SIEETWQQVMPQYPFVYQFVDEELEKLYKAEQRLTGIYTSFTVLAIIISCLGLFGLAS
                     FTTEQRTKEIGIRKVLGASIPKIIIMLSTEFLKLVLLSNIIAWPVAYFLMSRWLQDFA
                     FRVDIGIMTFILAGTTALCIALITVSYQAIKASSANPVDALKYE"
     gene            18081..20477
                     /locus_tag="PK754_01315"
     CDS             18081..20477
                     /locus_tag="PK754_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB08174.1"
                     /translation="MLYSYIRTAFRNIYRHKSVSAINLIGLTTGMTACLLIALYVHDE
                     LSYESQHTKADRIYRLNCTYYLPKNAGTEAYATTGPGVATAIAKDYPEIASVVRIRKI
                     DDFTLQKRNAIERFYETVMFADSTLFDVFSLPLIYGESKNALSSPLSMVISKSMALKY
                     FGKENAIGESFIVNDTLSCRIAGIIDDPRKPSHLEFNMIVSMVSMPVMHINLESWWNF
                     SYHTFVLLHPGTDVASFEEKMKNISRRYILSQEQNSGYSQEYNITNLRHIHLDSHLRS
                     ELSENGRRAYVYTFAAIGGLILLIASINFINLTTARVMTRAREVGVRKVVGAHRRHLV
                     YQFFIESALMIIITSGLCIVTAELTLPWLNTVTGKKLFIDIFNDPIISIILFTLTSVV
                     CIGAGLYPALILSSFAPLSIIKSFKSSSRGILLRKVLVITQFVISIGLIAGIVLVHRQ
                     IAFMRNAQLGFSKEQTMILALREPVGNPQLQMYQNEFGNIPGVQLVTASSRVPGKELG
                     NNVVRLGWDDKAEWSDMRFLSADYEFLAAYDLRIMTGRWFERTRPSDENEAFVINESA
                     VTRLGFVSAEDALGKPLRWQRKKGRVIGVLKDFHFMPMQHHIEPFIVVMANSHDANYL
                     SLKLRSDNMPAMVEQIHSTYTRLMPGQSFEYSFLDESFDRLYHAEDRFNEIIFVFTGL
                     AILVACLGLFGLVSFTTEQRTKEIGVRKILGATTAHIIALIAKDFLWLIMIAVILATP
                     LAYYAFSTWLHNFPYRTDIVWWAFPVAGCIAMVLAAITMSVQSVKASSANPVDALKYE
                     "
     gene            20534..22897
                     /locus_tag="PK754_01320"
     CDS             20534..22897
                     /locus_tag="PK754_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB08175.1"
                     /translation="MLFNFLKVSLRNMWRNRAYAAINIIGLTVGLTACFIIGVYVIHE
                     TYYDRFHEKSERIYRIHSDTRGFGLSNASSPALFSELYKYNAIASATRVFRHWFDPLI
                     SRDKENGFIESQFFFADSSVFDVFSLTLLAGEGGEALGRPMTVLLTASTARKYFGKQS
                     PVGSILKYNAEHEFTVAGVIDDFPSTSHVRPDFIASMGSTEKIFWPTFLKSSGNLVKT
                     YIVVRPEASVENLAADLTSIYTTRYGKDNRVTLSLMPLTDIHLKSTVEKDFSINNDIR
                     YVQLLGIIGLIIILISLINYTNLTAARSLTRSKEVGIRQTLGGFRSMIVIQFLCESFL
                     FVLLGLVFSMVLSEMLFPAINRMTSIHLSFSLLESTHWIMIAAAVVLLAITGGVYPAW
                     IVARFKIVHTLKGRVEHVRGSWLRKGLVISQFVSGVILIVATSVIWHQMQFIREARLG
                     FTKEEVAIVRLMDPSVASNLPALKQRWLNIPGVQSVALANAMPGKTHAGDYISHRGTD
                     EDLAVAVNWIDEAFVPTMDLSIIAGRNISPAHPTDNDGSVLINEATARELGYPDPEKA
                     IGTLIALQGTDGGKQRTVVGVVNDFHYESFRHKITPLILVPQFGRCAYLMVRCNTQAL
                     DQTLDHIKTVWSEMATEQPFAFTFLDDSFQQLYAQDRRWSLVVGAGASAALIIACLGL
                     FGLVIFNVQRRVKEIGVRKILGATIGNIIRLLTKEYLLLIGAANMIAWPIAYYWMQEW
                     LTQFVYRPSFPWLAFPLALVATMSMAMLTIGFQAFKAAVTNPTFTQI"
     gene            23031..25442
                     /locus_tag="PK754_01325"
     CDS             23031..25442
                     /locus_tag="PK754_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB08176.1"
                     /translation="MISNYIKIALRNLLKHKLYSVINILGLAVGIAACLVIMIFIKDE
                     LSYDRHHPQADRIHRITRSWNNANGETSLHLARVAPTVAPLMRNDFPTVPVTQILQDF
                     QTRLTYENNVFIENRLYFADEFAFTVFGFKTIKGNRQDALKNPNELWITESMAKKYFG
                     VVDPVGKTIRYENEIDLQVVGVLADPPPQTHFHYDFLVSFSTFKTLVPPDALTDWGSN
                     GYITYAMLPTDMTSVELEEKFISFVDRYIPPKNSAFPKPSMGTRLHVQKITDIHLYSH
                     LDSEIEPNGNILYIWIFSLVAVFVLMIACINFMNLATAKASERATEVGVRKAIGAHRG
                     DLIRQFLSESLVITAIAFLLAIGLVELSLPVFSAFIEKTLELTLVENYSMLIFVLAVA
                     FLTGILAGSYPAIYLSSFEAAKVLKEKSRFGSARSRFRSALVVAQFAISIILIIGIGV
                     IQSQLDYCRNTDMGFDKDRIVSLFIEQETRGKIPQVKTQLLQSPGIEAVAASSRIPSG
                     RLLDSYKMSVESEGTMKEIDFRIAAVRVDEDFFPTYGIPMTSGRNFSKDFSTDDTAAV
                     VFNESSVAAIGWKSSNDALGKTVIAGGRTMKVIGIVKDFHFESMHEKISPMVFFPNTG
                     KYARLSIRLRGARIPETIEYIKTRWAEYHHGIPFEFQFVDENFDQLYRAEERLGSILR
                     TFAALATFIACLGLVGLAAFMAERRTKEIGIRKVMGATTFNIVRLLSSDFAKLVLLAN
                     VIAWPAAYYVMKEWLATFVYHTDISILIFIVSGAVTLSIAILTVAFQAFSAANANPIR
                     SLKYE"
     gene            25473..27881
                     /locus_tag="PK754_01330"
     CDS             25473..27881
                     /locus_tag="PK754_01330"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB08177.1"
                     /translation="MLTNYIKIALRNMLKAKGYSAINILGLAVGLACCLLIVLFITDE
                     LSYDTHYANGDRLYRVYLNARVNNKDLVTVTTNSPLAAAMMKEIPEVETAGRLYSPGN
                     FTIRYGEKIFNEERIYFADPTMLDIFSVTVREGDVKNFDQPNMIILSESMAKKYFSDE
                     NPIDKVLRLDGKTDYKVIAVYNDVPGNAHLHFDFLASLTNREEGKNTQWLSNNFSTYT
                     RLKPGASTTGVESKMREMVMRYGGPELQRYANITFEQFFSQGGAYAYHLQPIRDIHLY
                     SNLENEQEPGGDITYVYIFSGIAIFILLVACVNFMNLATARSAGRAKEVGIRKVLGSE
                     RGQLIRLFLIESLVITTLAMIIGVLLAEMALPFFNELSGKSLHIGIATNPMLGIGLVL
                     AVLLVGVLAGTYPAFYLTAYQPIAVLKGTLASGVKNSGLRNVLVVFQFCVSIILIAGT
                     IIVYQQLQYIQNRNLGFQKEQILVIHNVWQMNKQGDAFKQSVLRTPGVASASLTSTLP
                     GHPTGNSAFQIEGDASNEPYLLWQVRTDFDFLKTMGIDMAQGRDFSIEHPSDSTAIII
                     NEAAVRLMNMPQPLGKRLTRFSSAPYEIVGVTQDFNFQSLRNPVRPISIFMNPPGFPA
                     PSFLAVRVKPDHLQETIQSIGQVWNQFQPGEPYVYTFLDEDFGKLYRTEERAGTIVTT
                     FSSLAIFIACLGLLGLAAFATERRTKEIGIRKVLGASEGHIIGMICKEFVILVTVALV
                     ISVPIAYYVMNRWLQDFAYRIDIGADVFILSGVIALTVALLTVSYTAIRAALSNPVTA
                     LKYE"
     gene            27874..28368
                     /locus_tag="PK754_01335"
     CDS             27874..28368
                     /locus_tag="PK754_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB08178.1"
                     /translation="MNNISSIEIRAIGNHDNPALASIIRTVMPEFGADGPGFAIHDAE
                     VNDMFAAYTRPRSAYFVVVADGVVSGGGGIAPLAGGDANVCELKKMYFLPGLRGRGVG
                     HKIITLCLQSAVDFGYKQCYLETLTGMDAAQHLYKQHGFRSIPQAMGQTGHYSCDRFY
                     IKDI"
     gene            28403..28681
                     /locus_tag="PK754_01340"
     CDS             28403..28681
                     /locus_tag="PK754_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08179.1"
                     /translation="MAEVVIQLPELNSKDRIEVECRINGKKQVLHYRVEIFAWEDCNE
                     PVADRALCLKRLVENYDPNWQLQQIGAYTDKTVQVMFREKHTPGTLIH"
     gene            28692..29354
                     /locus_tag="PK754_01345"
     CDS             28692..29354
                     /locus_tag="PK754_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB08180.1"
                     /translation="MITLKNIEKYYENKSIKNYVLRDINLTIQPGEFVSIMGPSGSGK
                     STLLHILGMLDEPTKGEYLFLDQEVHRISERQKTELHKNHIGFVFQSYHLIDELTVYE
                     NIETPLLYKKTSGSERKALVADILDRFNIVGKKDLFPSQLSGGQQQLVGIARAVITAP
                     KVILADEPTGNLNSAQGEEIMQLFKKLNQEGTTIIQVTHSEKNAAYGNRIINLLDGFI
                     QK"
     gene            29385..29906
                     /locus_tag="PK754_01350"
     CDS             29385..29906
                     /locus_tag="PK754_01350"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB08181.1"
                     /translation="MFWKIKALLYARFRHVFPFGYFLNKENAALIKLLQPTIRPDDYV
                     LDAGCGDGNATRMLLKIHPQVRVMGIDQSPSMANPIEHSGRTEIRIASLDAIAEENHT
                     FDAVMCIGVTEYIDDIPRVLLEIKRATKPGAPMVVTFAPPVFWNYLRFGYGKTLRLIS
                     TTEATHFLQKTAG"
BASE COUNT         7967 a         7202 c         6640 g         8379 t
ORIGIN      
        1 caatatcatc ggcttactcg acaatccgac cagcggtgaa tatcattttg tcggacatga
       61 agtatcgcgt tattcggaac gtcagcgttc caatttgcgt aaagccaata tcggttttgt
      121 gtttcagagt tttaacctca tcgacgaact gacggtgtat gaaaatgtcg agttgccgct
      181 tttgtatctc ggtatgacgg catcggatcg taaaaaacgc gtcgaagaaa cgctggaaca
      241 tatgcaaatc atgcaccgcc gcaatcactt tccgcaacaa ctttccggcg gtcagcagca
      301 gcgtgtggcc gtgtcgcgcg ccgtcgtcgc caaacccaaa ctgatcctgg ccgacgaacc
      361 tacgggtaac cttgattccg cgcacggtga agaagtgatg cgtctgctga ccgacctcaa
      421 caaagccggt accaccatca tcatggtaac gcactccccg tcgcatgccg aatatgcgca
      481 ccgcatcatt cacctcttcg acgggcacat cgtgacggag aacatgaaag aagcagccaa
      541 gatctgacca cgaacggatg cacacgacga gaaacaaagt aaaactataa ggcggggact
      601 atgatcagga attatctgaa aatcgcggta cggaatatgc aaaaaaatgc tttgcatact
      661 ttttttaacg tattgggact ggcgatgggt atcgcatgct gtatcacggt atatatattc
      721 gtggatacga tgctcaatat ggataccgcg catcgcgagg ccgaacgaat attcacgatc
      781 aatcacgttc gcctgatcaa taaccgcgaa gaattctggg gattgtccaa aggaccgctt
      841 ggcccggcta ttaaaaatga ctttccggaa gtcactgatt ttatccgtct ggatgacaat
      901 aatgccgtgg tcaagcgcgg cgatcttatc tttaacgaag atattcgttt tgctgacgcc
      961 aatttctttt ccatgttcac cttttcgttg gcttccggcg tcggcagcga tctggctcag
     1021 aaaaacgcca tttttttgag cgaagaaaaa gcgctgaaat attttggcga tcaaaatcct
     1081 gtcggtcaaa cgttgacttt gatttttgaa ggcgaaaaac gtttggagtt tgtcgtcaaa
     1141 ggtgtggcta aaaaatttcc ccgcaacacc agtttcgcat tcaactttct cgtcaattat
     1201 gaaaacatca aagacattta cccccaagcg gatcgctgga cttacaacgt cagcgctaca
     1261 tttatcaagc tgcatcgtcc ggaagatgcg ttcaaagtag aacaggggct ttcccgatat
     1321 ttggcgcttc aaaatgaaag caaccccaat cagcccgcgc agcgttttta tctcgacaat
     1381 ctgctcacga tgtctaaaaa ttcgtggcag gtacgcggtg atatttccgg cggcaccaat
     1441 ccgcacggca tttacacact gactttcatc gccgcgctgg tcatgctgat ggcctgcttt
     1501 aactatatca atatagccgt ggcctcggcg gcacgtcgtt ttaaagaaat cggaattcgc
     1561 aaagtcgtcg gcggaacccg cgggcaactc gtcaaacaat tcctgacaga aaattttatt
     1621 accacgctga cggctttagg tttaggtgtc gtgtttgcag aaactcttac cataccattt
     1681 tttaattcca tcatacaagg caacacggta ttagcgtttg atatttccgc tgatctgcgc
     1741 gtagttattt ttttagccgt actgacttct tcgatcggac ttttagcagg gctgtatccg
     1801 tcgttgtaca tgagttcgtt caaacctgtt aacattctca aagatgttca aaaaatagac
     1861 ggcaaccgtt ggttgggtaa aacgcttctc gtattacaat tttccatttc actttttgcg
     1921 gtcatcgccg gcattatttt tacacaaaac gccgaataca tcaaaacttt ggattacggc
     1981 tacgaagacg atcagatcgt cgctttgtat ttcaaacaat ccgcgcagtt tcatcaattt
     2041 gaacaaaaaa tacggcagga ctcgagaatt cacgccatcg ccggttccgc gcatcacgcc
     2101 ggacgcaata tcatgggaaa tgtcgtttat gaatccaacg gtgcgccatc ccaagctaac
     2161 gtcatgcgcg tgggttccga ctacatacag actttggctt tgcgcatgaa acaaggacgc
     2221 gcttttgatc gtgcgatgac gaccgacgcc gaaaatagcg ccatcgtcaa tgcgaccttg
     2281 gtcaaggcac aaggatggac ggaacctata ggacagcgcc tcaaaatgaa cgacaaagaa
     2341 ttgactgtcg tcggtgtcgt ggaagatttc ttttaccgtt cgtatcaccg cgccatagat
     2401 ccttgtatcc taacgatgat accggatgac gaataccgct acatgtccat caaagtagcg
     2461 ccggaacacg ttttatccat tcgtccgtta ttggaatcaa catggcgcga aatcatgccg
     2521 gacgaaccgt ttgacatgat ttatcaaaat gaaatttttc tggaagatct gacggaagac
     2581 gagcaagtaa aaaacgtatt tctgtatgtt gccatcatga ccttaatgat cgccgcgatg
     2641 gggctatttg ctttggtatc gctatctatc gccaaacgga cgaaagaaat cggaatacgc
     2701 aaagtattag gtgcatccgt agcacacgtg atacaccttg tcaatcggga cttctattgg
     2761 ctgatcggta ttgcttcggt gattattttt ccgattgctt tttttctgct taccaatcta
     2821 ctcaacagca tctacgccgt acatgtggat atcacggcat tacccttcat actcagcgta
     2881 ttgatattcg gcctcgttgc catcatcaca atcggcatgc gggtatatgg cgtcgcgaag
     2941 gaaaaccccg tacatgcttt gaagtatgaa taaccgacgg aaaactacat cgtaacaagt
     3001 cattctgttt gacgatgttt gattttgaaa atttttattt cggagtacct atatgatcaa
     3061 aaattattta caaaccgcat ggcgggtttt gctgcgcaat cgcggatacg cgtctattaa
     3121 tattttcggt ctgacacttg gtatgacctg cgcattcatg atctttctga tcgtacgctt
     3181 tcacctcagt ttcaacgacc agcatcgttt caaagatcgc ctttatcgcg tcacgacgac
     3241 ggataaacgc gccgctcagg aagacgaaac gagcatgggc accccttacc ctttatcggg
     3301 tttactgcgc accggttttc cggaaatcga aaatgtggcc gtaaccgact acgtacgcaa
     3361 cggtcaggtt ctgatatcgc aacagaacgg cgagcccaaa aaattcagcg aaaccggcgg
     3421 catcgcattt gttcagcctc agttttttga catgtttgat tttccctgga tcgagggcac
     3481 gcccaaaact ctggacgaac tgaataccgt tgctttgagt caaacgttga gtcagaaata
     3541 tttccccgaa ggtaatccca tcggaaaaat cattcgctta gataatgagc acgatctcaa
     3601 agttgcgggc attttcaaag attttccggc taactccgac ttcccatttc agatggcgat
     3661 cgcgtttgaa acctcgcggg ccatgtaccc caacaacaaa acagaatggg gttctacctc
     3721 gagcaatacg aacacatacg tactatttaa agaaaatttt gaccttcgcg cggctgaggc
     3781 caaacttaac gaagaagcca aaaaacaccg ggagatacgc ccgggttttg atcggatttt
     3841 tttatttcag ccgcttgacg ccattcatta cgacagtaat ttcggttcgt atgccggcgc
     3901 caccataagt caaaacacga ttatcacact cggtttgatc ggatttttcc tgatagtaac
     3961 cgcgtgcatt aatttcgtta acctcgcaac ggcgcaagcc gtcaaacgcg ccaaagaagt
     4021 cggtgtgcgt aaagtattag gcgctaatcg cttccaattg gtcgctcaat tttacggtga
     4081 aacgattttg atcacgctgt ttgccgtcat ccttactttc gggcttaccg aatttagttt
     4141 ccctaaagtt atggaatggt tggatctgac tattccttcc gtcttgatct ggaacgccga
     4201 cagtcttttg tttattgccg cactctcggt gatggttatc tttttagccg gcctttaccc
     4261 ggcgctcgtc ttatccggat tcggtcctgc acttgcgctc aaaagtaaga tcacttcgta
     4321 tcattccgga ggcctgtatc tgcgcaaagg tctcgtcgtt ttgcagtttt cgatttcaca
     4381 actgctgatc atcggcacga tcattgcgac gacgcagttg gattttttca acaaacaaga
     4441 ccttggtttc aataaagaag ctgtggtcat cgtaccgata cacgagcgga caaaaacatt
     4501 acttgataat ttagagcatc aatggaaaag ccatcccggc gtggaatcgg tgagttttgc
     4561 ttacgccagt gcggcctcag gcaatcgctg gacttccaat gtcactttct taaaaaagga
     4621 tgaaccgatt gaattagacg ttgacctcaa aatggctgat gatcactacg cggacatttt
     4681 tggattacaa tttttagccg ggcgaaacta tgtagccgga gatacattgc gcgaagttgt
     4741 gatcaatgag acgatggttc gcaaactcgg attttctaat ccacaggatg ctatcggcat
     4801 tgaactcaag atcggagcac gtcgccgcgt cagcgttgtt ggcgccgtgc gtgacttcaa
     4861 tgcctcctca ctccatgatc cgatcgctcc ttgcataatt gctacgaaca gccggtttta
     4921 tcaggaggcg tccataaaga tcaacatgca acaggcaacg gatgtcttga gacacatcga
     4981 aacagtctgg aataccgcgt ttcctcaata tgtattcgac cgacgtttcc aagatgaaac
     5041 gattgccgag ttttacgaac aagagcaaat ctataccaac atgcttcgta ttttttcggg
     5101 catcgcgatt ttcatcggtt gcctcggtct gttcggtctt gtctcattta tggccgcgca
     5161 aaaaaccaaa gaaatcggcg tacgtaaagt tcttggcgcc tcggtcacgg atattttagg
     5221 catgtttgca aaagagttta tcgcgttgat cattttggct ttcatcatcg ccgctccgct
     5281 ggcttactac gcgatgcaac aatggttgca agatttcgag taccgcatca cccttggccc
     5341 ggctgttttt gccgccgcgg taatggtttc attgatcatt gccggagcca ccgtcggtta
     5401 tcaggtattt cgtgccgcac gtgccaatcc ggccgacgct ttgaaatacg aataaatttt
     5461 aatgctgtaa ttttcaggag aaaactgatg atacgtaatt atttcatcat cgctttcaga
     5521 aatctgtaca gaaaaaaaat gtacacattt atcaatttat tcggcctgag cgtttctatc
     5581 gcggtgtgca tcgtcggtta ccttatatgg gattacagtt accactttga tcagtttcat
     5641 gaaaacgccg aacgcattta tcatatcgga accacacggg tgatcaatga acaggatcaa
     5701 atttttggag tttctcctct gccattggcc ccggcgctgg cacgggattt ttcgggcgta
     5761 aaacgcgccg tacgatttgc caatcaggta gccatcgtcc gagtcggtga taaagtattt
     5821 aatgaatcca tacacttcgc ggacgaacaa tttttcgaga tgtttcgttt tgaattggaa
     5881 cacggccgct acgatgcttt aaaaagcaaa accgatctta tcatcaccca tgacatggcg
     5941 atgaaatatt tcggtgaagc gaacgtggtc ggtcaatcca tgacgataag ttacgccaat
     6001 gaagttaaac aaacttatac catcggcggt gttctaaaaa ctcttccgcg taactcgagt
     6061 ttgcgattcg gttttttgct ttcttctgaa attttatcgg atctgggtgt tgacgaacgc
     6121 aacaactgga gccacgtaac gggcgctacc tttatcgaag tccatcaggt cgaaacaatc
     6181 caacagatca aaggcagcga gtccaaatat atcgaattgg ttcgatccgc caatccacga
     6241 tttcctataa gccatctcgt catcacaccg ctcaaggacc tggctttaga gggagaaagt
     6301 ttccgaagcc acgatttgca ggacgcaccg ccgccttctg caagaatttc tcttttcgtc
     6361 acttctatcc tccttcttgt catgtcgtgc tttaactaca tgaacacctc gctcgccatg
     6421 gctacggcaa gacttaaaga aatcggtatt cggaaagtaa tgggtagccc gcgtacacag
     6481 ctgatttttc aatttttgac ggagaatgtg ctgctttgtg ttcttgctat ctttgcagct
     6541 ttcggattgg ctgagttttt ggttccagga tggaatgcgc ttttttatga tattcaactt
     6601 tccatggacg aatcgaaatc tcccggcttg tggttatttc ttatcggcct cgtcggctta
     6661 acagcggtcg gtggcggatt gtatccggcc tatgtcatca gcgcgcacaa tccggctaaa
     6721 attctgcaag ggcatcaatc taaaactacg gcgagctgga tcatgaaagt gatgctgaca
     6781 ttacagtttt cggtctctat catagccgtc gtcggcagcg tcgtgttttc acaaaatgca
     6841 gagtatcaaa aaaatttaga cttggggtat gaacccgaaa aactcatcgg cgtggttatt
     6901 cagaagagtt ctatgtatcc ggtactttcg aatgcgctca tgcagaattc acagatcgaa
     6961 agcgtatcag ccggacgaaa tcacatttta tattcatcct ggcgaagaag tataaaagtc
     7021 gttgatagag atatcgaagc ggaattttta tatgtacgtc ccgggtatac tgaaaccgtc
     7081 ggattgaaat tagccgaagg tcgttttttt gatgtttccc aaccgcttga tttgacggag
     7141 tcgatcgtga tcaaccgcat gatggccaaa gagcaaggtt gggaaaatcc tatcggacaa
     7201 accatggtta ttgattcggt gacgtgccgt gtcgtcggtg tcatagaaga tttttatatt
     7261 cgcggcccgt tccggccgat actaccctgc gtttttaaag cggccaacga agacagttat
     7321 agatttgtaa tcgtcaaagc cgatcataat aacatccatg cggcgatgcg ttctgtggaa
     7381 gaaacatgga aaaaactctt tcctgagatg ccctttgacg ggttttatca ggaaacacaa
     7441 accgctaatg cggccgaact tagcaagagt atcatgctcg tgtttttctg cgtagcgctt
     7501 atggcgatca ttatttccgg tatgggtctc tttgcgctgg tgtcactaaa tatcgtacgg
     7561 cggaccaagg aaatcggtat ccgtaaagta ctgggcgcat cggcactaca gatcatgcgt
     7621 atgatgaatt cggattttat tattctttta ttgatcgctt ccgtcatcgc cgatgtagcc
     7681 ggctttttcc ttttcaaagc gcttctggga agtatttaca gataccacgt ggaagtcgga
     7741 atcatggctc tcatactggc caacattgca gttctcctga tcggattggc gacgatcgga
     7801 ggtcgtgttc tcaaaacggc gcgcgccaat ccggtaaacg cattaaaata tgagtaaaaa
     7861 ttaactgtat tgtatactgg agtctcatga gtcgtaatta tttacttatc gccattcgaa
     7921 accttttcaa acaaaaaggt tttgcattga tcaatacatc agggttagcc gttggcatcg
     7981 ccggttgttt actgattctt ttatttgttc gtgatgaatt aagttatgat caataccatt
     8041 ccaaagcccg tcgcattttc cgactacaaa ccaatgtaga tgtttccggc aacttactta
     8101 cactggccac tacttcctat cctatggcgg aagccttgaa aactgatcac tcacaagtgg
     8161 agtctgctat tcgtgtaatg cggtggaatg atccgatact atctcaaggc gacatacgct
     8221 tgaaagaaac gtcaatgctc tgggccgacg ccggtgtttt cgatgttttt tcatggccca
     8281 tgatatacgg cgacccgaaa acggctcttc aagaaatcaa ctccgtcgta ctcagtgaaa
     8341 gcgcctccaa aaaatatttt ggcgatgaca atcctatcgg aaaaattctg cgctacgatg
     8401 gtcgtattga tttattagtc aaaggcgtga tcaaagatat accttataac tcgcatgttc
     8461 gatttgaagt cctggccaat atacaaactc tgctggacgt cgtaggccga gaaagcatga
     8521 gccactggca tgccttttac caactgcaga cctatctgct attgcgaaat actttcgatg
     8581 cccaatcgtt gatcgaaagt ttacccagct ttgttgacaa atacctcaag ggggagtttg
     8641 cggaatctgt cggtcgaact tatacaatgg aactaacgcc cttgaccgaa ctgcacctca
     8701 gtacatcccg caaaggtgaa ttttcaaccg tgggcagcat gaattatgtt tatgtattct
     8761 ccggcattgc tttttgcttg ttatccatag cgtgtattaa ttttattaac ctctctacgg
     8821 cacacgcaac aaagcggcac cgtgaaattg gcttgcgaaa agttcttgga gccaatcgct
     8881 ctcaactgat ccgacaattt ttaaccgaaa caacattact tgtttttatt tccataacag
     8941 tagcattgtt attgatagat attgtattgc catattttaa cacgcttgcc ggaaaacatt
     9001 ttacattttc aatttttgcg gattacacga ccttacttac gataccggga ttggtcgtga
     9061 tcgtagggat attagccggc atgtatcctg cattttattt atcgcgtttt aacccaatcg
     9121 agtccgtgcg aggatcacat tattcgggca ctaaaaaaac gcgcttcaga cacgctttgg
     9181 taagtatcca atttgtcgta tcaatagttt tattgatcat aacggccgtt gtttttaggc
     9241 aactggattt tattcaaaac aaaaatatcg gcatagacaa aaatcaaaca cttgttatac
     9301 cggtcacgga ccaacaaata cgcgcgcgtt accactcgtt acgtgagcaa ctcacaaccc
     9361 agccgggcat tgaaaatgtc gctatatcag gttttatgcc gggtattaga gtgcccggat
     9421 cgccgttgca aaaaataccc actggaccat cagacaagtg ggagttttcc actatccctg
     9481 cggatgaaaa ttttgtccct actctcggca tcaatatcat agccggtcgt aattttacta
     9541 aacttaatcc cacggacagt gctgaaagca tgctgatcaa tgaagcagcg gctcgtgact
     9601 taggttggac accggaagaa gcgatcggta aacctttggc ttggggcgga gatcccggat
     9661 tcaatgttcg ggttatcgga gttataaaag atttcaacta tacctcacta catcagccga
     9721 tcaaaccact agtcattatc ccgacggcat tatggccaaa tagttataat tttctcagca
     9781 ttcgcatcaa taccggccac ctctccgaaa ccattcatca tattaacacc gtttgggaac
     9841 aacgaatgcc ggagacgcca tttatttatt catttctcga cgatgatttt tcaaaactat
     9901 atgccggtga aaacaaattg ggtattattt ttctgatctt cgcatctctt gctatttttg
     9961 tcgccagcct aggtctgctg ggtttggcta catttgtatc cgaatctaga agaaaagaaa
    10021 tcagcatacg taaagtagta ggcgctgacc tgagaacttt gatcatatta ttgctcggtg
    10081 attttctgat gccgattacg atttccactc tcataagctg gccgatagcc tactatatcg
    10141 catcggcttg gctccaaaat ttcgcatatc gtaccgaacc gacttggtat gtttttgtcg
    10201 cggcgacttt gttttcaata ggtatagcag ctctgatgct catacaatat gtaatcaaga
    10261 cagcccgggt caatccggcg gtaacgctca agtcggaata gacgttttct atttatcaca
    10321 acctctcaag aatcgtgagg ttttatgttt aaaaattatt tccaaacgcc aatccggtaa
    10381 acgcattaaa atatgagtga tcaaggataa tattatggta aagaattttt tgatcgtggc
    10441 catgcgcacc atgcgcaaac acaaatcgta caccatcatg aatgtgaccg gcctcgcaac
    10501 cgggttggcg tgcttcatgg tcatagccat gtatgttcgc cgcgaaacgg cgtttgatac
    10561 ctttcataaa aaccccgacc gcctttttcg tgtagcgctg tatcgggaaa acaccggctc
    10621 tccacaacct atcgcaaccg tatctcacag tgtcgccgac atccttcgcc gcgaatcccc
    10681 gggtattgaa tccatggcac gtttatttcg atttgatcaa gacgccgtca ttctggcggg
    10741 cgaaaaaaaa ttcaccgagc cggatttgat gttcgccgaa ccggatgttt ttgacctttt
    10801 gagtatcgaa ctgctgaatg gcagccctgc taatgcttta aaaaatccta cggatgccgt
    10861 tttatcggaa gccagtgcac gcgcctattt cggacatacc gacgtgatcg gacagagtat
    10921 ccgcgttcgc atcaatacac gctacgccga tttcaagatt acgggcgtat tcaaaaacta
    10981 tccttccaat tcgcattttt ataccaacgt attattggga tttgacggag taaaaaatta
    11041 cgtcggtgat gtggattttt ttcaaagctg gtttaattat cccatgtgga catacctgcg
    11101 cattaaggat atccaggacg aagtatcggt taatgccgca ctccatcaga tcgttgaacg
    11161 ccactttcct caaacgcgca agccggcgca catgcagctt cagcgcatca cggatatcca
    11221 ccttcattca caaatgagcg gagaaattag accgaatagt aacatttttt acatctacac
    11281 gtttacggtt atcgggctac ttcttttgat cgtagcttgt gtgaactaca tcaacctttc
    11341 gaccgctcaa tcggaacaac gtacgcgcga agtcggcgtg cgcaaagtga tgggcgcccg
    11401 ccgcagggaa ttaatatttc agtttttgat ggaatccgtt ctgcaaagta tgcttgcatt
    11461 gggtcttgct ttgggattgg ccgaagtgct ggcatccgtt ctcaaccgat tcacggatct
    11521 ccaactcacg ttcgattatt cggatccgtt tttatttata ttttcggcgt ttctgatcgt
    11581 ggtcgtcggc gtcggtgcag gtttttatcc ggctgtcgtt ttgtccggtt atgcgccaat
    11641 ccgaaattta agaatgaaag cttttcggcg ccggtttcct cttcgaaaaa tattagtcat
    11701 tgttcagctt tcggtgaccg ctgcactcat cagcgcggtt tggatcattc agcttcagat
    11761 caactacgtg cgctccaaag aacttggatt taataaagat cacaccgttt tgatacgtgc
    11821 gatcggaagt acggtagctt ctaatctgaa ttacgatcgt ttcaaatacc aatgtgaaca
    11881 attacccggt gtgatttcag taacacgtca ttcatgggtg gcaggcgaag ggtttcgaat
    11941 tcggtccgtc ggttttgatt ctttttcgga agagcagcgc caaccgatac aatttatctt
    12001 tgtaggcgag gattatctca aaacgtacga tctgaaactt attgaaggcc gtgatttttc
    12061 cgcacagtac ccggccgata cagccggcgc atatatcgta aacgaagccg ccgtcaaagc
    12121 atacggactg accaacccta tcggacgcat agccgtcagc ggtgatcgcg gcccggtgcg
    12181 cggacccatc gtaggagttg tcaaagattt tcattttgcg agtttgcacg aaaccatcga
    12241 accgatggtc atcggattat tccgattccc gatgccttac atctccgtac gatttgacgg
    12301 ccaagcaacg ccggaaacca tcggcgccat cgaacgttta tggaataaca ttgatacgga
    12361 acgcccgatg gactatagct ttttagatac acgtatcgag cgcgtctatc gttttgaaaa
    12421 ccaactttcc gccataacca aattattcac gattttatcc atgatcatcg gctgcatggg
    12481 tttattcggc cttatcgcag ccatggctga aaaacgcacc aaagaaatcg gcattcgcaa
    12541 agtattgggc gcttcgattt ggggcataat cggaatgttt gcccgcgaat tttcggccat
    12601 ggtcattgtc gccacgcttt tagctgttcc gactgtgtat tccggtctat cccaatggct
    12661 cgaaggattt gcatatcgtg tggagctgac ggctgaaccc tttatcgtat cctttatcgc
    12721 gatcttgttt ttaacggtta ttacggtcgc tcatcatgca ttgaaggccg cgaaaactaa
    12781 tccggcggta tcgctcaagt cagagtagac gatttctatt tatctcaacc tctcaaggat
    12841 cgtgagattt tatgtttaaa aaaatcataa caacgtaaaa atcgcatagg atatctatgt
    12901 tcaaagttta cctgaaaatt attttacgaa actttctgaa agaacgttcc ttcgtctgga
    12961 tcaacctgat cggattatcg gtcgggctgc tctgttgttt tacgatctta ctctacgcgc
    13021 gtaaggaatt ggcctacgaa aattttcacg ttaacaagga tcacatttac cgtgtattgc
    13081 cgcgaatcca aggcggtgcc aacgattatg aacaaatttg gacgccggca tggcttgccc
    13141 cgtccgctaa aaataaaatt cccgagattc gcctcgcatc gcgatacatc catttgggat
    13201 cggaacaact gatcacacat gaaaaccaac gtatgcttac ggacggatta gctttggccg
    13261 atccggattt tttacacatg ttctctttta cggaaccgtt cacgtccgat atgaatccgc
    13321 ttagcgatcc ttcggcactg atcgtctccc gttccgctgc gtatcgtata tttggagatc
    13381 aatctccgat cgggaaaacc ataacgctca atggttcaca cgtttttacg atcaaagccg
    13441 tttttacgga tgtaccggtc gaatcccact tgcaatttga aatgttgggt aattttcagc
    13501 gcattcgcga aatctgggga atgaacacga caccggaaga aaataccagt tggaattttg
    13561 caacctacct gatgacaacg cctcaagccg acatcagcgc ggtacagtct aaggtattga
    13621 atattttcga agaacgtgca ccgcgaaaaa atcagcgcac ggtccgacta cagtctctca
    13681 acgatattca tttcacgcaa ggtatccgcc gtgataacgc cgacggaaat tggacttcac
    13741 tgatcgcgct ttgcagcgtc gccgtattgg tcgtgctcat cgcatgtttc aattttacca
    13801 atctgatgac ggccaaagcc atgcgacgcg ccaaagaaat ccccgtacga aaaatttccg
    13861 gcgcaagccg atggctcata gcgctgcagt ttatcagcga agcgctaatg ctgtcggttt
    13921 ttgctaccgt cttggctatc gggatgctgg aaggcttact cccgctttta cgtcattatc
    13981 tcgacatatc tattcctttt acctataccg aacacggtca ctggttaggc attatgatcg
    14041 gcgtcggaat attgaccggg ctcatcgccg gtttttaccc cgcatgggtt ttatcatcct
    14101 ttgaacccgc gcgcaccctc aaaggcgtgg ccaaacttcc ggtggccaac aaactccgca
    14161 aaggattggt tttatcgcaa ttcaccgttt cgatcattct ggcttcgtgc acggtgattg
    14221 tttggcaaca aatcaaccac atgaaagaat ttaacctcgg ctttcaaaaa gaacagatta
    14281 tcgtgacttc agccccgggt tcattgcgtg aaaaacctga aaccgtgcgt caacagctca
    14341 ccaacgacgg ctcgatcata tccgtaacat ttgccaactc cgtacccggc aatgcttttt
    14401 cgcatggccc gtatgaaata ctgggcggaa cggaaaacgg acgcatttct atcaatacaa
    14461 tatatgccga tgcggaatat ttgaagacgc ttaacatagc gctatccgaa gggcgctatt
    14521 tttatccgga acatgcagcg gagaacacga attattatgt ggtcaacgaa gcttttatga
    14581 aagcttacgg aaaatcggat tttcaaacga tgcgcttgcg ttcattggac ggaaatcatg
    14641 ataaaccggg attaatcgtc gggattgtca aagacttcca ttacgcatcg ctcaaaagcg
    14701 ctatcgcacc attggtcctg cgctacgctc cggcagaatc ctggcgcatg attattcgcg
    14761 tcgcaccaca acgaacaaaa caggctataa caaaactttc gtccgtgtgg caaactatgg
    14821 cgccagaaat tccgatgacg taccgctttc ttgatgaggt gtttgacaaa caatataaac
    14881 aggaagatcg cctgcatcac ctgatgatcg gcttttcggc actggccatt cttattgccg
    14941 ggcttggcct ctatagtatg gtggcgtatt acaatgagca gcgtaccaaa gaaatcggaa
    15001 ttcgcaaagt tttgggcgcc acgatcacgg atgtactgta tctgatgtcg cgcgattatg
    15061 tcggaatggt catcctggca aacatcattt cattccctct gatttatttt atgatgaatc
    15121 actggctgga cgattttgcc tatcgcgtta ccgttcgatg ggaaatcatg gctattacgg
    15181 gaatattgac attaggattg acactcatca tggtgggaat acaatcctgg cgcacggctt
    15241 ccgccaatcc gatccacgca ctcaaatacg aataaacgaa ccttatcgaa taaaagcagg
    15301 aacatgtcat gctcaaaagt tatctcacta tggccttgcg tacgttatcc aaaagcaaag
    15361 cttattcttt catcaatatc gccggtcttg ccatcggact cacgtgtacg acgctgatac
    15421 tcctgtgggt gcaagacgaa ttgtcttata accgttttca tgaaaaacta ccgcgcctct
    15481 accgcgtgat ggaaagtcag cactatgcca atggtgaaat ttttacgaca tcggctacgc
    15541 cggctccgct gctgtaccat cttaaagagc gctttcctga attcgaaggt atttcccgaa
    15601 tgaatttttg gaataatata cttatcgaat acggcgaaaa acgcatgtat gaaaacgacg
    15661 gtctcggtgt ggaccccgat tttcttacca tgttttcttt tccattaatc aaaggcgatc
    15721 cgttaacagc gctcaatcaa cccgacgccg tagtactcag cgaaaccctg gctaaaaaat
    15781 atttcggaaa tgaagaccct ataggtaaaa ccatcaaaat gggtggccgc gacttattta
    15841 aaatcacggc tatagctgcc gatcctccgt tgcaatccag cattaaattc aatcttttgt
    15901 ggtccttgtc gtatctgaaa gaaacgcaac cggataaaag caattgggat aataactggc
    15961 tgagactgtt cgccgtttta cagcccaatc aaaaaggcac ggatgtaagt gaaaaaataa
    16021 aaaatgtgat tattgatcgt aatcctaata tggcggtccg aattgaatta ttcttacaac
    16081 ctctaagcga catgtacctg tactcggatt tcaataaaat accgccacgc atcaactacg
    16141 tttacatctt atcaggtatc gcatttttaa tacttttgat cgcatgtatt aattttacca
    16201 atctcgctac ggctcgctcg atcaaacgcg ccaaagaagt cggattacgc aaagtcgtag
    16261 gggccgatcg ttttcaactc attcgccaat ttctgatcga atccgtaacg atcacgttag
    16321 tcgccatgat gttatcgctc attctgatcg agctgctgat tcctcttttt aacgatctct
    16381 ccggaaaaca attatcctta cgttgggacg acatacagac gtttagtttg atgtgtgcga
    16441 tggttctatt cacgggattg gtttcaggat tgtatccggc tatggtactc agccgttacc
    16501 aacctgctct cgtactcaaa ggttcattca gcaagagcat acaaggcatg cgccttcgaa
    16561 aaatcctcgt atcgcttcag tttacattat ccatcgcgct cgtcatctgc accatcatcg
    16621 catatcagca gcttcagtat atgacgaata aaaacctcgg ctacgacaag gaacatctag
    16681 tttacattcg tttactggat gatacggcca aacaataccc cgtactcaaa gaaaaacttc
    16741 aacagaaacc gggtatcgta tcggtttctg ccgccgatca gacaatgacc gggtttggaa
    16801 gtaacacatg gggattaaaa tggcgcggtc aacaagaagg ccaacgcata ctgactactt
    16861 ttctgcgtgt ggactatgat ttttttgaaa cgcacggcat tcaagtttct gaaggacgat
    16921 ttttctcgaa aaattttgcc gccgacagcg gcgcctttat tgtcaacgaa gcggctatcc
    16981 ggcaaatgaa gttggagaat ccgcttcagg aaacgataac gctatggaac acgcagggcc
    17041 ctatcgtcgg tgttgtcaaa gattttcatt ttcaatccgt acatcaggaa atcacgcctt
    17101 tgatttttat actcaaaccg gaacgcgtga atgtaatgac cgttcgcatc gggccatcag
    17161 aaaccactaa aactttagcg tctattgaag aaacgtggca acaagtaatg cctcaatatc
    17221 cttttgtata tcaatttgta gacgaagaac ttgaaaagtt atataaagcc gagcaacgcc
    17281 tgaccggaat atatacgtct ttcaccgtac tggcgatcat catatcctgt ctcgggcttt
    17341 tcggattggc ctcttttacg acagaacagc gcaccaaaga aatcggcatt cgtaaagtgc
    17401 tcggcgcctc gattccgaaa atcatcatca tgctttcgac ggaattccta aagctcgttc
    17461 tattgtccaa tattatcgcc tggcccgtag cgtatttcct gatgagtcgc tggcttcagg
    17521 attttgcgtt tcgcgtggac atcggcatta tgacatttat tctggccggc accacggcgc
    17581 tttgtattgc attgattacc gtcagctatc aagcgatcaa agcttcatcc gctaatcctg
    17641 tagatgcgct gaaatatgaa taagggattt catactaatc caagaaagga aatggcatgt
    17701 accatcgctt aacacgcaat attttattga tcgtttttat tattgcagcg atagcatttt
    17761 taagtatgcg acacgaagac cttttttttt gcaatgattc gtatttcccg ggctcatcaa
    17821 attcatttca caacgttcgt catccccatt atctccggat gcatacaaat atatcaaagg
    17881 aatcggatga gaattttttc acagatgatc tgtgtaattc cgaaaaatac cgtcgaggct
    17941 tttatcatag aaaaatatcc aaaacacatg aacgatacga aatgcgcagg cattgggatc
    18001 ggttaaatac ccatcaagaa tggatacggc gctaaatcta atatgagttc ataatattaa
    18061 accatcctca aaggaagttt atgttataca gttatatccg tacggctttc agaaatatct
    18121 atcgacacaa aagcgtctct gcgatcaacc tcatcgggct gacgaccggc atgacagcgt
    18181 gccttctgat cgcattatat gtacatgatg aactgagcta cgaatcacaa cataccaaag
    18241 cggatcgcat ctatcgtttg aactgtacgt actaccttcc taaaaatgca ggcaccgaag
    18301 catatgccac gacaggaccc ggcgtagcga cagcgattgc caaagattat ccggaaattg
    18361 catcggtagt tcgcatccga aaaattgatg attttacgct tcaaaagcgt aacgccatcg
    18421 agcggtttta cgaaaccgtg atgttcgccg attcgactct tttcgatgtt tttagtcttc
    18481 cactgattta cggcgaatct aaaaacgccc tctcatcgcc gctttcgatg gtcatcagca
    18541 aatccatggc gctaaaatat ttcggaaaag aaaacgccat cggtgaatcc ttcatagtca
    18601 atgatacgct ttcgtgccgc atcgcgggca tcatagatga cccgcgcaaa ccatcccatc
    18661 ttgagtttaa catgattgtt tccatggtaa gtatgcccgt aatgcacatt aacttggaga
    18721 gttggtggaa ttttagctat cacacgtttg ttttattgca ccccggtacg gacgtcgctt
    18781 catttgaaga aaaaatgaag aatatttcac ggcgttatat tttatctcag gaacaaaatt
    18841 ccggttattc gcaagaatac aatattacca accttcgcca tattcatttg gattcgcatt
    18901 tacgatccga attaagcgaa aacggcaggc gggcttatgt gtatactttc gccgccatcg
    18961 gcggactgat cctactgatt gccagcatca actttatcaa tcttacgaca gcccgtgtca
    19021 tgacacgggc gcgcgaagtc ggtgtccgaa aagtcgtggg cgctcaccga cggcatctgg
    19081 tgtatcaatt tttcatcgaa tccgcattaa tgataattat tacttcaggg ctctgcattg
    19141 taacagccga gcttacgttg ccttggctta acacggtgac cggcaaaaaa cttttcatcg
    19201 atatttttaa tgacccgatt ataagtatca tcttgtttac gcttacctcg gtcgtatgca
    19261 tcggcgccgg tctttatcct gcactgatac tctcatcctt tgcgccactg tccatcatca
    19321 agagtttcaa atcgtcatcc cgtggcattt tgcttcgcaa ggttctcgta attacacagt
    19381 ttgtcatatc gatcgggttg atcgccggta ttgttttggt gcaccggcag attgcattta
    19441 tgcgaaacgc ccaacttgga ttttctaaag aacaaaccat gatcctcgct ttacgcgaac
    19501 ctgtcggcaa tccgcagtta caaatgtatc aaaacgaatt cggcaatatc cccggcgttc
    19561 agttggtgac ggcatcctca cgcgttcccg gaaaagaact cggcaataac gtcgttcgtc
    19621 tgggttggga tgacaaagcc gaatggagtg atatgcgttt tttatcggcc gattatgaat
    19681 ttctcgccgc ctatgatctg cggattatga ccggtcgctg gttcgaacgt acacgcccca
    19741 gcgatgaaaa cgaagcgttt gtaatcaatg agtccgccgt tacgcggctt gggtttgtat
    19801 cagccgaaga tgctctcggt aaaccgctgc gttggcaaag aaaaaaaggg cgtgttatcg
    19861 gagttcttaa ggatttccat tttatgccta tgcagcacca catcgaaccc tttatagtcg
    19921 ttatggccaa ttcgcatgac gccaactatt tatcgctcaa attacggtcg gacaatatgc
    19981 cggcaatggt agaacagatt cattcgacct acacgcgctt gatgccgggg caatcgtttg
    20041 agtactcttt tctggatgaa agttttgacc gattatacca tgcagaagat cgttttaacg
    20101 agatcatctt cgttttcacg ggtctggcta ttctcgttgc atgccttggg cttttcggat
    20161 tggtatcgtt cacgacggaa cagcgtacta aagaaatcgg cgtgcgaaaa atactcggcg
    20221 ccacgacagc acatatcatc gcattgattg ccaaagattt tctttggctt atcatgatag
    20281 ccgtaattct cgcaacacct ttagcgtatt atgctttcag cacatggctt cataatttcc
    20341 cctatcgaac agacatcgta tggtgggcgt ttcctgtagc aggatgcatc gcgatggtac
    20401 tcgcggcaat aacaatgtcc gtacaatccg tcaaagcttc atccgccaat cccgtggatg
    20461 cactgaagta tgagtaaaaa gttttgaata aatacgttaa tcgctgcatc aaaaaaacac
    20521 cgaaaggccg ccaatgctgt ttaatttcct aaaagtcagc ctgcgcaata tgtggcgcaa
    20581 ccgtgcttac gccgcgatca atattatcgg tttgaccgta ggtctgacgg catgtttcat
    20641 cattggcgtg tatgtgattc atgaaacata ctacgaccgg tttcatgaaa aatccgaacg
    20701 catctatcgt attcattcgg acacgcgggg ttttggttta tcgaacgcct cttcgccggc
    20761 attgttttca gagttatata aatacaatgc gatagcaagc gcaacacggg tctttcgtca
    20821 ttggttcgat ccgttgatct cacgcgataa agagaacggt tttatcgaaa gtcaattttt
    20881 ctttgccgat tcgtccgtat ttgatgtgtt tagtttaact cttctcgccg gtgaaggggg
    20941 agaagcgctt gggcgaccga tgaccgtatt attaaccgct tcgactgcgc gcaaatattt
    21001 cgggaaacag tctccggtag gatcaatatt aaaatataat gccgaacatg aattcaccgt
    21061 cgccggcgtt attgacgatt ttccgtccac ttcgcatgtg cggcctgatt ttatcgcttc
    21121 gatgggttcg acggaaaaaa tattctggcc gactttttta aaatcaagcg gcaaccttgt
    21181 aaaaacctat atcgtcgtac gcccggaggc atcggtcgaa aacctcgcgg ccgatctgac
    21241 aagtatttat acgacgcgct acggtaaaga taatcgtgtc accctcagcc tgatgccgct
    21301 caccgacatt catctcaaat ccaccgtcga aaaagatttt agtatcaaca atgatatacg
    21361 ctatgtacag ttattaggta tcatcggatt gattatcatt ttgatttcgc tgatcaacta
    21421 taccaatctt accgcggcgc gaagcctcac gcgcagtaaa gaggtgggta ttcgccaaac
    21481 gctcggcggt tttcgctcga tgatcgtcat ccagttttta tgcgaatcat ttttattcgt
    21541 tttgttagga ctcgtttttt cgatggtcct ttcggaaatg ttgtttcctg cgattaatcg
    21601 tatgacctcc attcatctca gtttttcgtt attggaaagc acacactgga ttatgatcgc
    21661 cgcggccgtc gtcctgctgg ccataacggg cggggtctat ccggcctgga tcgtcgcgcg
    21721 atttaaaata gtccatactc tgaaaggacg ggtcgaacac gtacgcggca gttggctgcg
    21781 caaaggtctc gtcatttcgc aatttgtttc cggtgtaatc ctgatcgtag cgacgagtgt
    21841 gatctggcat cagatgcagt ttatccgtga agcgcgatta ggttttacta aagaagaagt
    21901 agcgatcgta cgtctgatgg atccttcggt cgcatccaat ctccccgcgc tcaaacagcg
    21961 ttggctcaat attccgggtg tccaatccgt agccttagcc aatgctatgc ccggtaaaac
    22021 acatgccggt gattacattt cacaccgcgg cacggatgaa gacctggccg tagccgtcaa
    22081 ctggatagat gaagcttttg tgccgaccat ggatctttca atcattgccg gacgcaatat
    22141 ttcacccgct catccgaccg acaatgacgg atccgttttg atcaatgaag ccacggcgcg
    22201 tgaactggga taccccgatc cggaaaaagc gatcggcacg ctgatcgccc ttcaaggaac
    22261 ggacggcggt aaacaacgaa ccgtcgtcgg tgtggtaaat gattttcatt acgaaagttt
    22321 tagacacaaa attacgccgt tgattcttgt tcctcagttc ggacgatgcg cctacctgat
    22381 ggttcgttgc aatacgcaag cattggatca gacactggat catatcaaaa ccgtctggtc
    22441 ggagatggcc acggagcaac cgtttgcctt cacgttttta gatgactcat ttcagcagtt
    22501 gtatgcgcag gatcgtcggt ggtctctggt cgtcggtgcc ggggcttcgg ccgctctgat
    22561 catcgcatgc ctcggccttt tcggattagt tattttcaac gtccaacgcc gcgtcaaaga
    22621 aatcggagta cgcaaaatac tcggcgctac gatcggtaac attatccggt tacttactaa
    22681 agaatatctt ttgctgatcg gtgcagccaa tatgatcgct tggccgatag cctattattg
    22741 gatgcaggaa tggctcactc aatttgttta ccgcccttcg tttccatggc ttgcatttcc
    22801 tttggcgctg gttgcaacga tgagcatggc catgttgacg attggttttc aggcattcaa
    22861 agcggctgtg accaatccga cattcactca aatatgaata atgcgtgtag ggtcaaaacg
    22921 catcattttt atggcaacct tatatagagt tcaggcgtat agttaaaagt ataatgcagc
    22981 atttggagta acaaaatgta ccgatatatc cactttgaaa ggcacggcgc atgatatcca
    23041 attatatcaa aatcgctttg cgcaatttac ttaagcacaa attgtattcg gtaatcaata
    23101 ttctcggtct tgctgtcggc atcgccgcat gtcttgtgat tatgattttc atcaaagacg
    23161 aattgagtta cgatcgacac caccctcagg cggatcgtat acatcgcatt acccggtcat
    23221 ggaataacgc taatggtgaa accagtctgc atctggcgcg tgtcgctccg acagtagcgc
    23281 cgctgatgcg caacgacttt cctaccgtcc ctgtaaccca aatccttcag gactttcaaa
    23341 cacgtctcac gtatgaaaac aacgtgttta ttgaaaatcg tttgtacttt gcggatgaat
    23401 tcgcattcac tgtttttggc ttcaaaacca tcaaaggcaa ccgtcaggat gctttgaaaa
    23461 atcccaatga actgtggatc actgaaagca tggctaaaaa atattttgga gtcgtcgatc
    23521 cggtcggcaa aaccatccgt tatgaaaatg aaattgatct tcaagttgtc ggagtacttg
    23581 ctgatccgcc gccgcaaacg cactttcatt atgactttct tgtttcattc agtactttta
    23641 aaaccttagt tcccccggac gcactcaccg attggggttc caatggctat atcacttacg
    23701 ccatgctacc gaccgatatg acctctgtag aactcgaaga aaaatttatc tcttttgttg
    23761 accgatacat tccgcccaaa aattcggcat tcccgaagcc aagtatgggt acgcgattgc
    23821 atgtccaaaa aataacagat attcacctat actcacacct tgattctgag atcgaaccca
    23881 atggaaatat tttgtacatt tggatatttt ctctggtagc agtgtttgtc ttgatgatcg
    23941 catgtatcaa ttttatgaat ctggcaacag ccaaagcctc ggaacgcgcc acggaagtcg
    24001 gggtgcgcaa agcgatcggc gcacatcgcg gcgaccttat ccgtcaattt ctgagcgaat
    24061 cgctcgtaat caccgcaatc gcatttttgc tggcaattgg tttggtcgag ctatcgcttc
    24121 ctgtattttc tgcatttatc gaaaaaacac tcgaattgac tttggttgaa aattactcca
    24181 tgttgatatt tgtactcgcc gtagcattcc tcacgggcat actagccgga agttatccgg
    24241 cgatatatct ttcgtcgttt gaagccgcca aagtactcaa agaaaaaagc cggtttggtt
    24301 ccgcacgctc aagattccgc tcggcactcg tggtcgccca atttgctatt tctattatac
    24361 tcatcatagg catcggcgtt attcagagtc agctcgacta ttgccgcaat accgatatgg
    24421 gattcgataa agatcgtatc gtttctcttt ttatagagca agaaacacgc ggaaaaattc
    24481 ctcaggtaaa aacgcaactc ctgcaatctc ccggtattga agctgttgcg gcttctagcc
    24541 gcatcccgtc cggtcgttta ttggatagtt ataaaatgtc cgtggaatcc gaggggacaa
    24601 tgaaagaaat tgattttcgg atagcggccg ttcgtgtaga tgaggatttc tttccgacct
    24661 atggtatacc aatgacgtct gggagaaatt tttcaaagga cttttccacg gacgatacag
    24721 ccgctgtagt attcaatgaa agttctgtcg ctgccattgg gtggaagtcc agcaatgatg
    24781 ccctggggaa aacggtcata gccggcggga gaacgatgaa agtaatcggc atcgtcaaag
    24841 attttcattt tgaatccatg catgaaaaaa tttcaccgat ggtgtttttt ccaaacactg
    24901 gaaagtatgc ccgcctttca atccgtctgc gaggcgcacg gattccggaa actatagaat
    24961 acataaaaac ccggtgggcc gaatatcatc atggtattcc ttttgagttt cagtttgttg
    25021 atgaaaattt cgatcaactt tatcgcgccg aagagcgttt gggttccata ctccgtacgt
    25081 ttgcggcact cgctacgttt atagcatgtc tgggtcttgt aggcctagcc gcttttatgg
    25141 ccgagcgtcg caccaaagaa atcggcattc gcaaagtgat gggcgcaacc acatttaata
    25201 tcgtgcgcct attatcatcc gattttgcaa aattggtatt gttagccaat gtgatcgcct
    25261 ggcctgcggc gtattatgtt atgaaagaat ggctcgctac attcgtgtat catacggaca
    25321 tttctatttt gatttttatc gtttccggag ccgttacgct gagtattgcg atattgacgg
    25381 ttgcgttcca ggccttttca gccgccaacg ccaacccgat acggtcccta aaatacgaat
    25441 aacatgattt atatattcag gagtacacac gcatgttgac caattatatc aaaatcgctt
    25501 tacgcaatat gctcaaagcc aaaggctatt cggctatcaa tattttagga ttggccgtcg
    25561 gtctggcatg ttgtttattg atcgttcttt ttatcaccga cgaactgagt tacgataccc
    25621 attatgccaa cggcgatcgt ttgtaccgcg tttatctgaa cgctcgcgtc aacaacaaag
    25681 accttgtcac ggtaaccacg aatagccctc tggcggccgc tatgatgaag gaaatccctg
    25741 aagtcgaaac ggccggtcgt ctgtacagtc cgggaaattt tacgattcgt tacggtgaaa
    25801 aaatctttaa tgaagagcgt atatattttg ccgatccgac gatgctggat atttttagtg
    25861 tgaccgtccg cgaaggcgac gtcaaaaatt ttgaccagcc caatatgatt attttatcgg
    25921 aaagcatggc caaaaaatat ttctcagacg aaaatccgat cgataaagtg ctgcggcttg
    25981 acggtaaaac cgattacaag gtcatcgccg tgtataatga tgtccccggc aacgcgcatt
    26041 tgcatttcga ttttttggcc agcctcacca accgtgagga gggaaaaaac acgcaatggc
    26101 taagcaataa tttttcaacg tacacacggt taaagcccgg cgcatccaca accggcgtcg
    26161 aatccaagat gcgtgaaatg gtcatgcgtt acggcggacc tgagttgcaa cgatatgcta
    26221 atatcacctt tgaacaattt ttttcgcaag gcggcgcata cgcttatcat ctgcagccga
    26281 tccgcgacat ccatttgtat tccaatcttg aaaacgaaca agaacccggc ggcgacatta
    26341 cgtacgttta tattttttca ggtattgcca tttttatttt gctcgttgcg tgtgtcaatt
    26401 ttatgaatct tgccaccgca cgttccgcag gtcgcgccaa agaagtcggc attcgcaaag
    26461 tgctcggctc cgaacgcggt caactcatac gtctctttct gatcgaatcg ctggtcatca
    26521 caaccttggc gatgatcatc ggcgtacttt tggctgaaat ggcattgcca ttttttaacg
    26581 aactctcagg taaatcgctg catatcggaa tcgcgaccaa tcctatgctt ggtatcggac
    26641 ttgttttggc tgtactttta gtcggtgtac tggccggcac ctatccggcg ttttacctca
    26701 cggcttacca accgatcgct gtactcaaag gaacactcgc ttccggcgta aaaaatagcg
    26761 ggctgcgtaa tgtccttgtt gtttttcaat tctgcgtatc catcatcctg atcgcgggca
    26821 cgatcatcgt ttatcaacag ctgcaatata tacaaaaccg aaatctgggc tttcaaaaag
    26881 aacagatcct cgttattcat aatgtatggc aaatgaataa acaaggtgat gccttcaaac
    26941 aatccgtcct gcgcacaccc ggcgtcgcaa gcgcatcgtt gaccagtact ttacccggac
    27001 atcctaccgg caacagcgca tttcagatcg aaggcgacgc gtccaacgaa ccctatctcc
    27061 tttggcaagt gcgaaccgat tttgattttc tcaaaactat gggcattgac atggcccaag
    27121 gccgtgattt ctcgatcgaa catccttcgg actccaccgc gatcattatc aatgaagccg
    27181 ctgtccgtct gatgaatatg ccgcaacccc tcggcaaacg cctgacgcgt tttagttcag
    27241 cgccgtacga aatcgtcggc gtcacgcagg atttcaattt tcaatctttg cgcaatccgg
    27301 ttcgcccgat ttcgattttt atgaatcctc cgggttttcc ggcgccttca tttttagcgg
    27361 ttcgtgtaaa accggatcat ttacaggaga ccatacaatc catcggccag gtgtggaatc
    27421 aattccagcc cggtgaaccg tacgtgtaca ccttccttga tgaagatttc gggaaactgt
    27481 atcgcacgga agagcgcgcc ggtacgatcg taacgacatt ttcaagcttg gctatattta
    27541 ttgcctgcct tggacttttg ggtctcgccg cttttgccac cgaacggcgc actaaagaaa
    27601 ttggtattcg caaagtgctc ggcgcatccg aaggtcacat catcggcatg atctgcaaag
    27661 aattcgtcat actggttact gtcgctttag tcatttccgt gccgatcgca tactacgtga
    27721 tgaatcgttg gctgcaggat tttgcctacc gcattgacat cggcgctgat gtatttattc
    27781 tgtcgggcgt gattgcattg accgtggcgc tgcttacggt aagctacacg gcgatccgcg
    27841 ccgccttatc caatcccgta accgcattga aatatgaata acatttcatc catagaaata
    27901 cgcgcgatcg gaaatcacga caacccggct ttagcatcca tcatacgtac ggtgatgccg
    27961 gagtttggcg ccgacggtcc gggttttgcc atacacgatg ccgaagtcaa tgatatgttc
    28021 gccgcttaca cacgcccacg cagcgcttat ttcgtggtcg tagccgatgg cgtagtctcc
    28081 ggcggtggcg gcatcgctcc tttagcgggc ggagatgcga acgtatgcga attaaaaaaa
    28141 atgtattttc ttcccggctt gcggggtcgc ggcgtcggac ataagatcat cacgctttgt
    28201 ctgcagagcg ccgttgattt cggttataaa caatgttatc tcgaaacatt gaccggcatg
    28261 gatgcagcac aacatttgta taaacagcac gggtttcgtt ccattcctca agcgatggga
    28321 caaaccggcc actacagttg cgatcgtttt tatatcaaag atatttgacc gcaatgccat
    28381 aaaaaaaccg aaaggacgca tcatggctga agtcgttatc caattgccgg aactcaactc
    28441 caaagaccgc atcgaagtcg aatgccggat caacggaaaa aaacaagtac tgcattaccg
    28501 cgtggaaatt tttgcgtggg aagattgcaa tgaacccgta gcggatcgtg cactgtgcct
    28561 caaacgattg gttgaaaatt acgatcctaa ttggcaactt cagcagatcg gcgcttatac
    28621 ggataaaacc gtacaggtga tgttccgcga aaagcacaca cccggcactt taatccattg
    28681 atagaggcat catgatcact ctgaaaaaca tcgaaaagta ttacgaaaac aaaagcatca
    28741 aaaattatgt cctccgggat atcaacctga ccattcagcc cggcgaattt gtttccatta
    28801 tggggccgag cggttccggc aaatccaccc tgttgcacat cctcggtatg ctggacgaac
    28861 cgaccaaagg cgagtatctt tttttggatc aggaagtgca ccgcatatcc gaacgccaaa
    28921 aaaccgagtt gcataaaaat cacatcggat ttgtttttca gagttaccat cttatcgacg
    28981 aactgaccgt ctatgaaaac atcgaaacac cgctgctgta caaaaaaacg agcggctcgg
    29041 aacgcaaagc tttggtcgcg gatattttgg atcgttttaa tatcgtcggt aaaaaggatc
    29101 tatttcccag ccagctatcc ggcggtcaac aacagcttgt cggcattgcc cgtgcggtga
    29161 tcaccgcacc gaaagtcatc ctcgccgacg aacctacggg caatctcaat tccgcccagg
    29221 gcgaagagat catgcaactt ttcaaaaaac tcaatcagga aggcacgacg atcattcagg
    29281 tcacccactc tgaaaaaaat gcggcgtacg gaaatcgtat catcaatctt ttagacggat
    29341 tcattcagaa ataatacttc gctgttacac attcttattt ttttatgttt tggaaaatca
    29401 aagcgcttct gtatgccagg ttccgacatg tatttccttt tgggtatttt ttaaataaag
    29461 agaatgcggc tttgataaag cttctccaac cgacgattcg acccgatgac tatgtactgg
    29521 acgccggatg cggcgatggc aatgctacgc gaatgctttt gaaaatacac cctcaggttc
    29581 gagtcatggg catagatcaa tctccatcca tggcaaaccc tatagaacat tccggacgca
    29641 ctgaaattcg aatcgcttca ctggatgcca tcgcagaaga aaatcatact tttgatgccg
    29701 tgatgtgtat cggggtcacc gagtatattg acgatatccc aagggtactg cttgaaataa
    29761 aacgtgcaac caaacccggt gcgccgatgg tggtaacgtt tgccccgcct gtgttttgga
    29821 attatctgcg cttcggatac ggaaagactc tacgtttaat atcaacgacg gaagcaactc
    29881 actttcttca aaaaacggct ggctaatcca atcacagagc gcaacttcga tgcaaaatca
    29941 atttcttgta cgaaattctg tctgatgaaa attactcttc gcaatgccgc atagcgaaat
    30001 tgaaaagata gtctcgacaa aatcgcctta cgccttggtt tcttaagaga acgcatttca
    30061 catcggctct caacttagtt atcgcataga cgtgccgtat cgaaatgaaa aataaaaact
    30121 attctcgcag agacgcaaag gttacaagac attttttgta cgttcatttc ttagcgtctt
    30181 agcgagaa
//
LOCUS       DAOQNQ010000010        29628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_497_length_29628_cov_6.508234, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000010 DAOQNQ010000000
VERSION     DAOQNQ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 29628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29628
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_497_length_29628_cov_6.508234"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1855
                     /locus_tag="PK754_01355"
     CDS             104..1855
                     /locus_tag="PK754_01355"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB08182.1"
                     /translation="MPFTSYVLRISAFLLVFISGVILASVFFNTAITLIMGAVVLAAF
                     VIAARWIAARREKPVEKYKVSGNTIRRAVTHPHTVETVSGSIVREEDHAMEILDDTQA
                     SLMFNQTADGVIAIMHDTLQAHSTFLYLCHDDSGECLPQSFRSESRDFIPEKSFRYGD
                     YAPFHQVIQSKESLVMDLHEPGTMPVPYYRTAVPVKHLVIVPLMRKGAVLGLLGVDRI
                     TDTALTEQEFDAIKQYGRLILLSIQSIDGVYMKNKLRRLSQSLREFSERLSLAGMETQ
                     TTACLKEQIQNTLRFDRMHIWLRHGAEEIRLVEAGGSAAYDKESMVRLDGTMIEKVMT
                     KRRRLYISDVTSIDRTSVNSELELNYLASVVVIPICDQDHCFGVLVIEQGQKNAYDVY
                     EVQFLETVCNSAGLALARIALDQHLSKNANEGERNASTVEQTFMKNVVSEIHRAKRFE
                     TSFSVLIFQVDYVKNIYEAFGVEAGDLALEKITEILKNGIRFIDSLARLSHDRFGVLL
                     VESKRKEAVECAGRILHALESAVIHSGDEEIPLTATVGVVTYGTDGTEADALMHRANV
                     ALVNAKKLNERRIACAD"
     gene            1862..2131
                     /locus_tag="PK754_01360"
     CDS             1862..2131
                     /locus_tag="PK754_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNB08183.1"
                     /translation="MVLGKVIGSVWATRKDQKLTGVKLLIVRSIGLDYKEKDAFVVAA
                     DSVGAGVGEVVLYCTGSSARQTEATENRPVDAVIMAIVDKMEVHP"
     gene            2131..2493
                     /locus_tag="PK754_01365"
     CDS             2131..2493
                     /locus_tag="PK754_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNB08184.1"
                     /translation="MANKINLADKFETFSDTWHPRIVGELNGQHVKLVKVSGEFVWHH
                     HDHEDEMFYVVRGCLKMEFRDRTEIINPGEFIIVPRGVEHRPVSDVETEIMLFEPATT
                     VNTGNIKNERTRENLQHI"
     gene            2490..2753
                     /locus_tag="PK754_01370"
     CDS             2490..2753
                     /locus_tag="PK754_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNB08185.1"
                     /translation="MNMARIIGTVWATKKHPSIEGCRLQIIQPVDSELKHVGGPIVAT
                     DSVGAGQPNELVYYVTSGEAPIPLDRKAPTDATIIGIVDRIDR"
     gene            2786..3631
                     /locus_tag="PK754_01375"
     CDS             2786..3631
                     /locus_tag="PK754_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB08186.1"
                     /translation="MAHYVEHNNDLIDKYLQEIGAVPLLTSEEEIALAQRIREGDERA
                     LEHLTKANLRFVVSVAKQYQGQGLSLNDLINEGNIGLIKAAKRFDETRGFKFISYAVW
                     WIRQAILQALAEQSRVVRIPLNKIGTLNKIGRVYSDLVQEFEREPSAEEIAEQLEISE
                     LEVHDTLQMSGRHISVDEPIYDSDNNTLIDVIENEQQAPPDHDLVNESLKIEIERALD
                     SLSARESEVIRLYFGLENDHPMTLEEIGVRFQLTRERVRQIKEKAIRRLRHTSRSHVL
                     RHYLG"
     gene            3849..6299
                     /locus_tag="PK754_01380"
     CDS             3849..6299
                     /locus_tag="PK754_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNB08187.1"
                     /translation="MKNNFSSRVQLVVKLSQEEAIRLGHDYIGTEHLLLGLIREGEGV
                     AVKVLKNLAMDLEKLKRAIEDMVKPVTDNTVIGNPPLTKRAEKILKSTYVEAKNFKSD
                     VIGTEHLLLALIKEKDCLAAQVLSMFNIDYETVRRELTNIMSGTQTPTNPTAKKPEPK
                     KSKTPALDHFGRDLTQYAEENRLDPIIGRDEEIERVAQILSRRKKNNPVLIGEPGVGK
                     TAIAEGLALRIVQKKVPRVLLGKRIVTLDVGALVAGTKYRGQFEERVKAIMSELEKTT
                     EVILFIDELHTIVGAGGASGSLDASNMFKPALSRGELQCIGATTLDEYRQYIEKDGAL
                     ERRFQKVIVDPPSVEETLEILRQIKHKYEEHHRVTYTDEALVQAVRLSDRYITDRHLP
                     DKAIDVMDEAGSRTHIMNIVVPEEIVALEQEIERVRTEKDFVIKNQKFEKAAELRDVE
                     KRLTEQLRVAKEEWEKKEDTQVVEVGEEQIAHVVAMMTGIPVNRVAQSETEKLLKMEN
                     VLCSRIIGQDDAIQSVTRAIRRTRAGLKDPNRPIGSFIFLGPTGVGKTQFAKELAYYL
                     FENADALVRIDMSEYMEKFSVSRLVGPPPGYVGYEEGGQLTEKIRRKPYSVVLMDEIE
                     KAHPDVFNILLQVLDDGILTDSLGRKVSFKNTVIIMTSNVGAREMSKIDGFGFSTKDN
                     GEDEYLRMKAKAEEATKKLFNPEFLNRIDEVIVFRALTKDNVLRIIDVSLDEVRKKLK
                     ERNMTLHLTDAAKEFVATKGFDPVYGARPLRRAIQKHIEDAVAEKILKGEFSDGSLVS
                     IELRDGELAFVEMAHEAV"
     gene            6340..6873
                     /locus_tag="PK754_01385"
     CDS             6340..6873
                     /locus_tag="PK754_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08188.1"
                     /translation="MLFGKKKIHARPGALDADAVKAAIKGLAVNDGATVERVDWDGSR
                     STVSGRVSHVATDYTSFVLVEEGSGESIEFYIEVGDIVKLDRVAISIDLDENQQNTFM
                     EVAQIIELLEALDHGDHIAVSYFDVIKGRGVSVKGIITLKDEYNKVFAVEYEENGEKK
                     DKHFNLNEDKIIDIIME"
     gene            7026..8162
                     /gene="dnaJ"
                     /locus_tag="PK754_01390"
     CDS             7026..8162
                     /gene="dnaJ"
                     /locus_tag="PK754_01390"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNB08189.1"
                     /translation="MAKRDYYEVLSVSKNASLDEIKKSYRKLAMQYHPDKNPGDKSAE
                     EKFKEIAEAYSVLSDEQKRAQYDRFGHDGMRGMGQQGFSDINDIFSHFSDIFEGFGFG
                     DAFGGGRGRSSRSNRGADLEVKLKLTLQEIAEGVTKKIKLRKRKSCESCNGSGAAAGS
                     SRKTCSVCHGSGQVRQVSRSVFGQFVNIAACRNCEGQGTIVEKPCSTCKGDGVTMGET
                     VISVKVPAGVTTGNYIPLRGQGHAAPHGGESGDVIVVLEEIEDEIFERHGDDVLLNVN
                     ITFPQAVLGDEVEVPTLFGKAKLTIPGGIQSGKILRMRGKGIPHLNGSGSGDQLIRVA
                     VHTPSKISDKERRLIEELMGYDNFRSPNSNDTSFFKKIKEAFTA"
     gene            8190..8720
                     /locus_tag="PK754_01395"
     CDS             8190..8720
                     /locus_tag="PK754_01395"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HNB08190.1"
                     /translation="MNFRNWGANAYKFFEYFGLTRQEAVVLCVLAGITLLGGLVGLIK
                     ETYRDDTIEITSVPYPKNEARPYPQKSENDSAFSVWFSRQDTLQTLFRKGVARDSIQR
                     FILKTNDAPARVVRINQADVQEISSLPGVGPKIAARIIEYRNKNGPFSKLEDIQNVKG
                     VGKKLIEKIRPFIDIN"
     gene            8724..9263
                     /locus_tag="PK754_01400"
     CDS             8724..9263
                     /locus_tag="PK754_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08191.1"
                     /translation="MASIPKTRITDYILKTIIVLLFVFLVWSVVSPVQAEKADRNKIR
                     LTRAKMKALLAVQKAYFVSDSSFTSDNGKLFDFVNKKGKDKVPDEVFTPVIEALMRFP
                     EYSEYFKDVTPATYREQYFESLTDNPMNGQKFIIETGLKDGRKTVHIKPSSVDSEIQR
                     IGAVIDSRVTWNEKLELTY"
     gene            9279..10367
                     /locus_tag="PK754_01405"
     CDS             9279..10367
                     /locus_tag="PK754_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08192.1"
                     /translation="MSQMSIQSDRGDLDHDAIDQDYARRAGEYRNLAFGLSFHGQQIH
                     FAEILRSEDGFSVRKMGTIQTTMKFGTGIEGVERNVQGLFAYLKGCLEDNGIRAKRMN
                     ISLNTNLVTLHKVLVESERSESDFQDYIKWEFRQQVTDDFDQYILNTVPLEKTSPAPM
                     DPMLMVGIRRRYADSLRSVLDKSKIQFSNMDVDILCTHATYEVNYDISYHGMTVLAEC
                     KTGSVTLLLCKDYDVQHVYQFTVSPKSTPQKIGELINAHIPNMMQAYHATAGNTNSVV
                     GRLILCHDLAQEVLPYIEADFNPSVIEPFRKIKLPAEFRPHEKNEDSESASADADEKD
                     NKSQVVIAPPDYAVYAEAVGAAIKLLMD"
     gene            10369..11355
                     /locus_tag="PK754_01410"
     CDS             10369..11355
                     /locus_tag="PK754_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08193.1"
                     /translation="MIKINLLENHFAPPAQHEKTDSDTSAGKTITIEAKKSQRRLLFI
                     ILIFVALVGGLTYGWMERYAVVAWLEQYTGPLHLLPEQDMETAAKAAEEARQEKIRNL
                     YMYNTIRYQERYRRFLFRTDSIVSANSQKWHLTSLGVEESNYAFDVFGKTQKDLEDFT
                     VLFLESKSIDETKPQAIKPTSLVKGLGFKRTMSGTLKTVSTPSDSEIVALKIIYLDLE
                     KAKQKILDGARNAGLKILQDGKMTSSKGVVMSTHSGELKLEGSVSDYFKYMAKLNEMR
                     FNFEIGEIDIQYADYSARRKADKKKLVPDTIFMKYSILLPRQSESADSTKTK"
     gene            11418..11981
                     /gene="rsmD"
                     /locus_tag="PK754_01415"
     CDS             11418..11981
                     /gene="rsmD"
                     /locus_tag="PK754_01415"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNB08194.1"
                     /translation="MRIIAGIRRGLVLNAFDEEIIRPTKDRVKESVFNILANIIDVTD
                     TQILDLFAGTGNLGIEALSRGAAKSTFVELHSKAVHVLRNNVIKCRFEQQIDVITGDA
                     LEFISSVAVKKYDIVFADPPYAMRIGAMIAKKIDAGQWLNPGGVLVIETSPEEIENEW
                     LPAHGLTMHKQKTWGESAVSILMNKAN"
     gene            11984..12469
                     /gene="coaD"
                     /locus_tag="PK754_01420"
     CDS             11984..12469
                     /gene="coaD"
                     /locus_tag="PK754_01420"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010286602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNB08195.1"
                     /translation="MRRAIYPGTFDPLTLGHLDVIARAATLFDEIIVAVANNSKKKPL
                     FSPEERVAMIAEATKDMANVRCEPSEGLIVDFAVSRQVHVLIRGLRVISDFEYELQME
                     HVNRRLNSQIVTMFLMPGEKYAYLNSTIVKEVAQYGGDLSSFVPAFIEQRIKEKLKVQ
                     K"
     gene            12471..13406
                     /gene="lepB"
                     /locus_tag="PK754_01425"
     CDS             12471..13406
                     /gene="lepB"
                     /locus_tag="PK754_01425"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNB08196.1"
                     /translation="MSIFGKKEEPKKNNPVPGKPLKEMTFTERLVENVKSFGWALCVY
                     YITITFVVQNFTIPTGSMEVSLLPGDFIFANKFIYGAKSPENIPFTDIKLPQFQLPAF
                     KEPQQGDVVVFRFPHPEMMPSQNGLDYIKRCVAAPGQTLYVRNKVLFVDGEALSVKFD
                     LPGVHFDPYERSEGQVFPRHMGSGDNFGPFRVPKKGDQIELNLTNYELVRYLSLRDGK
                     KLEMRGGEWTVDGVKSTYYTVGQDYFFMMGDNRDNSLDSRYWGPVPRSHIAGQGMLVY
                     WSNTDAAKQSSIPMKVLMMFNFFSVKWHRIGTIIR"
     gene            13483..14184
                     /gene="lepB"
                     /locus_tag="PK754_01430"
     CDS             13483..14184
                     /gene="lepB"
                     /locus_tag="PK754_01430"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009109356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNB08197.1"
                     /translation="MEKPLSDNHNKTTELFQIAFVSVFIAIIVRLFFVQAFKIPTGSM
                     EDTLLVGDLILVNKCIYGVKTPATIPLIFTDIDLAQYQFPAVREPKQGDVIVFKFPPD
                     PTVDYIKRLVALPGQTVEIKDKIVYVDGKDFSEIIRPPGLKFEDPEIIPRNKGYESVF
                     PRDAGSRDNYGPVTVPEDSYFVLGDNRDRSYDSRHWGFVPKDHLIGQAVMIYWSTASQ
                     DNFDIRWDRLGQMIH"
     gene            complement(14191..15078)
                     /locus_tag="PK754_01435"
     CDS             complement(14191..15078)
                     /locus_tag="PK754_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HNB08198.1"
                     /translation="MLKSYIQLPPDFSFDECLVFLNRSDREILHRVHNKSLTKAFRIG
                     HETCLTSITVKSDKMMLRFERDISNEAARFIRSEVSEWFDLSCDLANVYRLMKKDPKL
                     FALTKRFHGLRLIGIPDLFEALAWAVIGQQINLAFAYTLKQRLIDVAGQSLTHDGLPY
                     GLFPDPATVADLRVDTLRKLQFSTRKAEYLIHIARQIADKRIEKTALQNYSLSQIVER
                     LTAERGIGAWTAHYVAMKCLRQANAFPIGDAGLHQAVLHIYQMKKKPTLIQIEKKSKL
                     WQGCEAYAVFYLWRSLQET"
     gene            15169..15582
                     /locus_tag="PK754_01440"
     CDS             15169..15582
                     /locus_tag="PK754_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNB08199.1"
                     /translation="MRVHTHYLWFNTKQRREYINITDEVESALNVSGIKEGMALVSAM
                     HITAAVYVNDAEDGLIKDIDKWLDHLAPANPNYLHHRTGEDNADAHLKNLLLHHEVIV
                     PVTGGRLDFGPWQQIYYAEFDGQRKKRVVIKIMGL"
     gene            15661..16266
                     /locus_tag="PK754_01445"
     CDS             15661..16266
                     /locus_tag="PK754_01445"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNB08200.1"
                     /translation="MAFTLPSLPYAHDALEPHFDKMTMEIHHGKHHNAYVTNLNNAVA
                     GKPEENLSIEEICKNISKYPVAVRNNGGGHFNHSLFWTVLGPNAGGEPTGALADAIQS
                     AFGSFADFKTQFANAGATRFGSGWAWLIVKEGKLAICSTPNQDNPLMDIAEVKGAPIL
                     GLDVWEHAYYLKYQNRRPDYITAFWNVVNWNKVAENFANAK"
     gene            16300..16872
                     /locus_tag="PK754_01450"
     CDS             16300..16872
                     /locus_tag="PK754_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNB08201.1"
                     /translation="MDLTKELKDNVLLTTVEAVYDWGRRNSLWPMPFGTACCAIEMMA
                     TLGPRYDMSRFGSEAIRFSPRQADLMIVSGRVSIKMMPVLKRIWDQMPEPKWCISMGA
                     CASTGGFFNTYTLIQGVDQFIPVDVYVPGCPPRPEGLIEGVMKIQKLIAKREAAYARD
                     IKDDGMYGDDKVGIAGRGKDLVAEIATGKF"
     gene            complement(16946..19129)
                     /locus_tag="PK754_01455"
     CDS             complement(16946..19129)
                     /locus_tag="PK754_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNB08202.1"
                     /translation="MSEQEILRLVAKELNIPVQHVTSTIQLLDLENTVPFIARYRKEV
                     TGGMDENQIRDIQDKMTYYRALEARKEVVLKSIEEQGKLTDELKARIVAATMLQEVED
                     LYLPYKPKRRTKGIIAKEKGLEPLALQILAQEITSGTLDEHAQPFINLEKGVTTVDEA
                     LQGARDIIAEIISEDADVRKDVREFTRENGILVSEVKEDPENEYEMYHTFSEPIAKMP
                     PHRILATNRGEREEKLRIKITVDEEACCNIIGYRHVKKQKSIFRDQVIEAIKESYFRL
                     IAPSIEREIRGEFTEKSEEHAIEVFGSNARNLLLQAPTKGKIIMGIDPGFRTGCKVAV
                     IDETGKYLEGETIYPHEPQKDALGSKRVLKQFIRKYEVSVIAIGNGTASRETESLVAD
                     LIADLKKEDSHLELVYTIVSEAGASVYSASKIAQQEFPDLEASQRGNISIARRLLDPL
                     AELVKIDPKSIGVGQYQHDVNQKRLAEQLTHVVESAVNYVGVNLNTASAALLTYVSGL
                     SSKTAQSIIAHRDKNGKFSRREQLMDVTGIGPAAYQQCAGFLRIPDGDNPLDNTSIHP
                     ESYDATQKLLKKFNIVDPSTAGSLLELQLRNAKMSFENIAGEVGVGVPTLKDILDNLK
                     KPGRDPREEMPKPIFKSDVLKMEDLREGMMLKGTVRNVVDFGAFVDIGVKQDGLVHIS
                     HLSNKYVKNPMEVVAVGDVVDVKVMSVDLKKGRVQLSMKEASRSY"
     gene            complement(19336..20451)
                     /locus_tag="PK754_01460"
     CDS             complement(19336..20451)
                     /locus_tag="PK754_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08203.1"
                     /translation="MKVLNYNTILMCLCVCLTACSEGMDTSSGDKQPPHFEWDPGSAT
                     VILKDMIQYGENNIAILSLHYDTNRYGLTGFTPNGIQKFQKFLPATHYIESSYFERNL
                     SYSNGIFTLVINPKDTGQNSLFIQLDTNGNITNQKELVQIKYASIFPMNNGEYLVRSS
                     IFDSLQFKTKLSKIDNDGELKWEKVMPVSFEYLHVANNRIYGKWNNDSIVSIDANGDV
                     LWKQSLMLPKGFLLCRVSSSDYRIARILYNPYNYIPDRLAIDAMDIDGAITPLDTIPL
                     NWSTDFTDFESLGANELVLLGRWGGSNKILLMRLQSDGSWVESHLAPDENNFSLVSFH
                     ANGDGTYLIMGAMYINSSYYSKTLIYKIDANGNYLPF"
     gene            complement(20448..21383)
                     /locus_tag="PK754_01465"
     CDS             complement(20448..21383)
                     /locus_tag="PK754_01465"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HNB08204.1"
                     /translation="MKKKSMTFVMVALFVGSACNQNNNNEFLGVFGILMPYASENATI
                     ILMRTYSQENYDEFIPEALQIKESNAEVIISSDSQHVTLSEVIPGRYVDTAGMLKYYP
                     GLIYKLEVKAKDGLSVHAQCVLPGVPIPDPETTPDSFSVAIELDTVRRIVSGYPYPVL
                     TQMYYLRSPTVSFDWSDATGAEAYYWEIGADTSYLKESDREFFIPNSKLFQTEKWIAY
                     SSLVLFDHWDSTTYRGLYNKPDNPWTWSGFYIDSEREFVYTSQLHITAIDRNFIDYTY
                     YNHKSNIRGGYGYFSAATRHKRPITVHVQSKWKQR"
     gene            complement(21386..24100)
                     /locus_tag="PK754_01470"
     CDS             complement(21386..24100)
                     /locus_tag="PK754_01470"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNB08205.1"
                     /translation="MNFYRVFFVAFFFTTYLTFAQEKEIASSSAQSAISLKQAVAELE
                     SRYHVRFSYVDQTIAKYTVRTPIKGDDLQQSLEYILIGKPLAYKRIGKNIIVLYEDDT
                     KKQEASGQFTSVRLTIVDARTSEGIYFANVTISGSRRGALSDAHGQAVVQHIPYGDYT
                     LRITALGYLPLDKTLHLSSQEITLDTIRLEPSDIRLDSTIVFGERETWRVSDAKLRVQ
                     PSIIVIDRRKINSTPSVLEPDLFRTLQTLPGVTAPNDLSNELYVRGGTPDQNLITMDR
                     AIVYQPYHLFGIAGIFNTDAIEQVNFSTGGFSAQYGNRLSSVIDVRTKSASPNKFSAT
                     GNASLLSSKITMDGQPSKHWYYLISLRRTYLDFATKAAVWLKIAPESIPYSFYDGLSK
                     VVYRPNTLNEFGTSLFFSRDKFFSKHRDYEYVFNEFNISRKLPMYSVGRLDYGWGNLN
                     AAVYWDWQAGDIFRSRVTISQSKATIKLDEDLYYGYDSGAPDSIRRRTDSLNALQEKR
                     PFDTRNYIVDRGFAWDTQWEWLNEHSMYLGVSLNLIEMSYYWKNFNAPPHYVQVFYDF
                     APDSFRYQRRTTNLAFYIEDMWKKGDWTIKPGIRIEHFSSNKRSLLISPRTAIRYDVS
                     PKLALKGSAGIYYQSLFTARERGYIGFLEVPFSTEDQVEKATHIIAGGEFFPNPNIRL
                     TADIYFKHFNRLYRKDHSSDSLHFDKGGGDAYGLEITYRRIGNKMSFEADYSLAWVTR
                     TFDQNTYVTNYDQRHTLNLLLSYRLPKNWTVDARWILASGRAYRPTEFYGATAYIEPG
                     SGRSLMEDNGYNISQENLDHLEFYDRYPLYHRLDVSLVKTVRFKTWTMKPYISIINTY
                     YSNNPLFYDYNYNGSIISNEPDPQRMNYSKRKGYGVPILPSFGTFFEF"
     gene            complement(24114..25070)
                     /locus_tag="PK754_01475"
     CDS             complement(24114..25070)
                     /locus_tag="PK754_01475"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNB08206.1"
                     /translation="MSENYYHQDDFIGRWIAGTLTDEEKKEFELWLKSNSHQQEFFNE
                     LTKIHWSQKSLEIPKAPTERMWSAIAEATTSKQVSRSWIRNTTQWAAAIAASIVIYFG
                     LESQFAYREITTSKAQQTEIVLSDGSIARLNGLSSIRYNSWRWPLTRSVTMHGEVYFE
                     VQKMKRPFNVTAENLNVEVLGTKFNVRDRADRSEVVCSSGKVRVSARNNSENTILTPG
                     LAVSMHQGHFSMPYSMDTTLVAGWSQGIWNYQSAPIYSILNDWKITYGLTITMDSIDT
                     KRTFTGLIHGSSSLSALKQLGLSAGFETTIVNDSTFFLKLKK"
     gene            complement(25621..26157)
                     /locus_tag="PK754_01480"
     CDS             complement(25621..26157)
                     /locus_tag="PK754_01480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HNB08207.1"
                     /translation="MTTEEIQLLTKKIRNSDALAFRTLFDFFHRPLYHFVFYKTYDRA
                     LSEDIVQDVFIRLWENRFNLREDLSIKSYLYTIAVNLTLNTIRGHTIRTGEGIEDPDT
                     QPHEETPLSVLEKKEFQQLLNSAIQKLPEQARIVFMMSRYDDLSYSEISERLSISVKT
                     VEAHIGRALKQLRTTLKH"
     gene            complement(26297..27850)
                     /locus_tag="PK754_01485"
     CDS             complement(26297..27850)
                     /locus_tag="PK754_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08208.1"
                     /translation="MCQFITLCLINLLFVFSSLQAGDGDKKPLSDVLKGIEKKHNVVF
                     SYVPALVQNVRVNTPSESDKLNHQLDYVFAQTDLTYRFIGTKEITLFKKTKTNSNIEA
                     TAQARLQAGRSRADEALSTMNKTLDGEQESGEHEDDSLPTDTTVSIDKNETSPSIVIP
                     TPMSPQTETQSVPTEKTEPHDLKNRSVRSEFLGMDFAPTTRIEPAYVPVPLHAISINI
                     ISTRLVSIEGVEVGLIGNYDRMYVNGLQIAGGGNRAYLHARGLQIAGGSNQTGDLSGV
                     QIATVNIADQTNGLQIGAVNISKKDHSGLQIGAGYNHAENVRGLQLASVNEASHVHGI
                     QIALVNRAKQVHGAQIGLFNFADENNGIALGLLNFIRNGQHHGRLARTERGFNHFSYT
                     HGSKYMYTIYDVAVRQLREPAQWALGGGIGAIVWPDRRLSLAIEATTHQHNKDKFWEH
                     RLNMVNTLKITPTIKLHKHLSVYAGASANVWVSRAENGFPAPTNAYNWDYKHTTIRAW
                     WGGLVGIYF"
     gene            27970..28671
                     /gene="pepE"
                     /locus_tag="PK754_01490"
     CDS             27970..28671
                     /gene="pepE"
                     /locus_tag="PK754_01490"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019330759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HNB08209.1"
                     /translation="MRLLLCSNSTNFGENYLDHWAAEIVDFLGAIKQIVFVPYALDDL
                     NAYTKKTRDRYAEMGIRVAGIHENDPRKMVTEAEAIHIGGGNTFRLLKKLYDEDLIDI
                     IRHRVSDGMLYLGASAGTNVATISIKTTNDMPIVYPPSFEALNLVPFNINPHYIDPDP
                     STQHMGETRDQRISEFHEVNNAPVLGLREGALIRREGDTLTLKGKNGAKLFTKGNTPQ
                     LNNTGADLSFLLRGF"
     gene            29055..>29628
                     /locus_tag="PK754_01495"
     CDS             29055..>29628
                     /locus_tag="PK754_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08210.1"
                     /translation="MLRQIILVITLFSAVLIAQDKKILTYEAFVDLRRVDEPAVSPDG
                     NWVLYNVRKYSLESNTYTSNIFLTSIDGKTSRQLTFSPTKDITPIWSPDGKTIAFLSN
                     RLGGFQIWTMALDGGEPKRVTNLSTQVSGNGLVWSPNGQYILITSDVFPDCETDDCNR
                     AHNDAAENSKVKAQLIGKLPFRVWDSFKDGK"
BASE COUNT         8288 a         6027 c         6939 g         8374 t
ORIGIN      
        1 tcgtattcca atcccatcgt ttctttcagc aaatgcgttg acttttttag gcaaaggggt
       61 taagttttgc cgaactaaca caagattgtg ttgaaataaa ctaatgccct ttacttccta
      121 cgtcctgcgc atttcggcat tcctcctcgt tttcatttcg ggagtcatcc tcgcgtcggt
      181 tttttttaat acggccatta cattgatcat gggtgctgtc gttctggccg cctttgtgat
      241 agctgcacga tggatcgccg ctcgtcgtga aaaaccggtt gaaaaatata aagtctccgg
      301 caatacgata cgacgcgccg tcacgcaccc gcataccgtg gaaaccgttt ccgggtcgat
      361 cgttcgcgaa gaagatcacg cgatggaaat actggatgat acgcaggctt cgcttatgtt
      421 caatcagaca gcggatggcg tgatcgctat catgcacgat acattgcaag cccattctac
      481 ctttttgtat ctatgccatg acgatagcgg cgaatgttta ccgcaaagtt tccgaagtga
      541 aagccgtgat tttataccgg aaaaatcgtt tcgatacggc gattatgcgc cgtttcatca
      601 ggtgattcaa tccaaagaat cactcgttat ggatttgcac gaacccggta cgatgcccgt
      661 cccgtattac cgtaccgctg tacccgttaa acatttggtg attgttccgt tgatgcgaaa
      721 aggcgctgtg ctcggtctcc ttggtgtgga tcgtattacg gacacggcgt taaccgaaca
      781 ggagtttgac gcgatcaagc agtatggacg cctcatactt ttgtctattc aaagtattga
      841 cggcgtgtat atgaaaaaca agctccgccg cctttcccag agtttacgtg aatttagcga
      901 gcgcctgagc cttgcgggta tggaaacgca aacgacggct tgcctgaaag aacagattca
      961 aaacacgctt cgttttgatc gcatgcatat atggttgcga catggcgcgg aggaaatacg
     1021 attggtggag gccggtggca gtgcggcata cgataaagaa tctatggttc ggctcgacgg
     1081 cacgatgatc gaaaaagtta tgacgaaacg tcgtcggctt tatatatctg atgtgacatc
     1141 cattgaccgg acttcggtaa acagcgaatt ggaattgaat taccttgctt ccgtcgtggt
     1201 catcccgatc tgtgatcagg accattgttt tggtgtgctg gtgatcgaac aaggccaaaa
     1261 aaatgcatac gatgtgtatg aagtgcagtt tcttgaaacg gtctgcaata gcgcagggct
     1321 cgcattggcg cgtatcgctc tggatcagca tttatctaaa aatgcaaacg aaggcgaacg
     1381 aaatgcgtca acggtggagc aaacgtttat gaaaaacgtc gtgtccgaga ttcatcgcgc
     1441 taaaagattt gaaacttcgt ttagtgttct gatttttcag gtggattacg tcaagaatat
     1501 ttatgaagct ttcggtgtag aagccggtga tttagcgctt gaaaaaataa cagaaatttt
     1561 aaaaaatggg atacggttta ttgatagcct tgctcgtttg agtcacgacc gatttggggt
     1621 gttacttgta gaaagtaaaa ggaaagaagc ggtagaatgt gcaggccgga tactccacgc
     1681 gctcgaatcg gcggtgattc attccggcga tgaagagatt ccgttgaccg caacggtcgg
     1741 tgtggttaca tatggtacag acggtaccga agccgacgca ctgatgcatc gtgctaatgt
     1801 tgctttagtg aatgctaaaa aacttaatga gcgccgcatt gcatgcgcag attaggaata
     1861 tatggttcta ggtaaagtta taggttctgt atgggccaca cggaaagacc aaaaactcac
     1921 gggcgtgaag ctgttgattg tgcgcagcat aggattggac tacaaagaaa aagatgcttt
     1981 cgtggtggcg gcggattctg taggcgccgg cgtcggtgaa gtggtgttat attgtaccgg
     2041 cagttcggca cgtcaaaccg aagcgacaga aaatcggccg gttgatgcgg ttattatggc
     2101 tattgtagat aaaatggagg ttcatccttg atcgcaaata aaattaatct cgcggataag
     2161 tttgaaactt tttccgatac atggcatccg cgtatcgtgg gcgaactgaa tggtcagcat
     2221 gtcaagttag tcaaagtttc cggtgaattt gtttggcatc atcatgatca tgaggacgaa
     2281 atgttttatg tggttcgcgg ttgtttgaaa atggaatttc gcgatcgaac ggaaatcatt
     2341 aatccgggtg aatttatcat cgtaccgcgc ggagtggaac accggccggt ttctgacgta
     2401 gaaacggaga tcatgctttt tgaaccggca acgaccgtaa atacagggaa tatcaaaaac
     2461 gaacgcacac gcgagaattt gcagcatata tgaatatggc gcgtatcatc ggcacggttt
     2521 gggctactaa aaaacatcct tctatcgaag ggtgtcgttt acagatcatc cagccggtgg
     2581 attcagaatt gaagcacgtc ggcggcccga tcgtggcgac cgactcggta ggcgccggac
     2641 agcctaatga attagtttat tatgtaacat cgggtgaagc gccgatccct ctggatcgca
     2701 aagcgcctac tgatgcaaca attataggaa ttgtagaccg tatagatcgt taaccatcgt
     2761 acaacaaaat gaaagaaggt tgtctatggc tcattatgtc gaacacaata atgatctcat
     2821 tgacaagtat ctgcaggaaa tcggcgccgt accgctcctg acatccgaag aagagatcgc
     2881 gctggcacag cgcatacgcg agggtgatga acgggcgctc gaacatctga ccaaagccaa
     2941 tctccgtttt gtcgtcagcg tagccaaaca atatcaaggc caaggcctct cactcaatga
     3001 ccttatcaac gaaggtaata tcggactgat caaagccgcg aaacgatttg atgaaacgcg
     3061 cggattcaaa tttatttctt atgccgtatg gtggattcgt caggccatac ttcaggcgct
     3121 tgccgaacaa tctcgcgtcg ttcgtatccc gctcaataaa atcggaactc ttaataaaat
     3181 cggtcgcgtg tatagtgatt tggtacagga attcgagcgc gaacccagcg ctgaagagat
     3241 cgcggagcag ttggaaattt ccgaactcga ggtgcacgat acattgcaaa tgtccgggcg
     3301 gcatatctcg gttgatgaac cgatttacga ttcagataat aatacgctga ttgatgtgat
     3361 cgaaaacgag cagcaggcgc cgccggatca cgatctcgtc aacgagtctt tgaaaattga
     3421 aatcgaacgt gcgctcgaca gtttatccgc gcgcgagtcg gaagtgatac gtttgtactt
     3481 tggtctcgaa aatgatcatc cgatgacatt ggaagaaata ggtgtgcgtt ttcagttaac
     3541 gcgtgagcgt gtccgtcaaa tcaaagaaaa agcgattcgc cgtctgcgtc acacttcgcg
     3601 aagtcatgta ttacggcact atttgggtta attaaaacaa ttttattaat ctttttctga
     3661 taccctgatt tcaaattaat cggctttttg tgttgtttta tgattggttt ttctcgcgtc
     3721 taagtgtgta atttttataa cattattact tcttgactaa tgtagcccga atctctatat
     3781 ttttttgaat tgtgcacaaa ataacgataa caagttttgt tatttggcct tttcagaatg
     3841 gagtcagtat gaaaaataat ttttccagcc gtgtccaact ggtcgtaaaa ttatcgcagg
     3901 aagaagccat ccggctcggc cacgattata taggtacgga gcatcttttg ttgggtttga
     3961 ttcgcgaagg cgaaggcgtc gccgtcaaag tgctcaaaaa tttggccatg gatttagaaa
     4021 aactgaaacg tgctatcgaa gatatggtaa aaccggtaac ggataatacg gtcatcggta
     4081 atccgccgct taccaagcga gccgaaaaaa tcttgaaatc cacgtatgtg gaagccaaaa
     4141 attttaaatc ggatgttatc ggtaccgaac atcttttact tgcgctgatc aaagaaaaag
     4201 attgccttgc tgcgcaagtt ttatccatgt tcaatattga ttacgaaacg gtaagaaggg
     4261 agctgaccaa tatcatgagc ggaacgcaga ctcctacaaa tcctacagcc aaaaagccgg
     4321 agcctaaaaa aagtaaaacg cctgccttgg atcactttgg tcgcgatctc acgcagtatg
     4381 ccgaagaaaa tcgccttgac ccgatcatcg gtcgtgatga agaaatcgaa cgtgttgcac
     4441 agatcctttc acgcagaaaa aagaataatc ctgttttgat cggagagccc ggcgtgggga
     4501 aaacagccat cgccgaaggg ctggctttgc gtatcgttca gaaaaaagtt ccccgtgttc
     4561 tattgggtaa acgaattgtg actctggatg tcggcgcgct ggtggccggt acaaaatacc
     4621 gcggccagtt tgaagagcgc gtcaaagcga tcatgagtga actggagaaa accacagaag
     4681 tgatattatt tatagacgaa ctgcatacga tcgtcggggc cggtggagca agcggctctt
     4741 tggacgcatc aaacatgttc aagcctgcgt tgtcccgcgg cgagctccag tgtatcggcg
     4801 ctaccacact ggatgaatat cgtcagtata ttgaaaaaga cggtgcactt gagcgccgct
     4861 ttcaaaaagt gatcgttgat cccccatcgg tcgaagaaac ccttgaaatt ttgcgccaaa
     4921 taaaacacaa atacgaagaa catcatcgcg tcacgtacac ggacgaagcg ctggtgcaag
     4981 ccgttcgttt gtcggatcgt tacatcacgg atcgtcatct tcccgacaaa gccattgatg
     5041 tgatggatga agcaggttcc cgcacgcaca ttatgaatat cgtcgtgccg gaagaaatcg
     5101 ttgcgcttga acaggaaata gagcgcgttc gtacggaaaa agattttgtt ataaaaaatc
     5161 aaaaatttga aaaagccgct gagttgcgtg acgtcgaaaa acgattaacg gaacaacttc
     5221 gcgtggcgaa agaggaatgg gaaaagaaag aggatacgca agtcgtcgaa gtcggtgaag
     5281 agcagatcgc gcacgtcgtt gccatgatga cgggtattcc tgtcaatcgt gttgcgcaaa
     5341 gtgagacaga aaaattactc aaaatggaaa atgttctgtg cagtcgtatc ataggtcaag
     5401 atgatgcgat acagtcggta acacgcgcca tccgtcgtac acgagcagga ctcaaagacc
     5461 ctaatcgtcc tatcggttcg tttattttcc tcggtccgac cggtgtggga aaaacacaat
     5521 ttgccaaaga attggcttat tatctttttg aaaatgccga tgcgctggtg cgtattgata
     5581 tgtcggaata tatggaaaaa ttttccgtga gccgtttagt cggtccgccc ccgggatatg
     5641 tcggctacga agaaggcggt cagcttacgg aaaaaattcg ccgcaaaccg tacagcgtcg
     5701 ttcttatgga tgagatcgaa aaagcccatc cggatgtttt taatattctc ctacaagttc
     5761 tagatgacgg catcctcacg gacagtcttg gccgcaaagt gagcttcaaa aataccgtga
     5821 ttatcatgac atccaatgtc ggtgcccgcg aaatgagtaa aattgatggg ttcggttttt
     5881 cgacaaaaga taacggtgaa gatgaatatc tgcgcatgaa agccaaagcc gaagaagcaa
     5941 ctaaaaaact ttttaaccct gagtttctca atcgtattga cgaggtcatt gtattccgcg
     6001 cactgaccaa ggataatgtg ttgcggatta ttgacgtatc gctggatgaa gtgcgtaaga
     6061 aactcaaaga acgcaatatg actttacatt tgaccgatgc ggcaaaagaa tttgtcgcta
     6121 cgaaagggtt tgatccggtg tatggtgcgc gaccgcttcg cagagcaatc cagaaacata
     6181 tcgaagatgc tgttgctgaa aaaatattaa aaggtgagtt ttcggacggg agccttgtat
     6241 ctatcgagtt gcgtgatggc gagttagcat ttgtggaaat ggcgcacgaa gcagtttaga
     6301 agtcagatga ttgtacttta taacggaggt ttttgaaata tgctttttgg aaagaaaaaa
     6361 atacatgcgc gtcccggtgc cttggatgcg gatgcggtca aagcggctat caaagggtta
     6421 gccgtaaacg atggcgctac cgtcgaacgt gtggattggg acgggagccg ttcgactgtt
     6481 tccgggcgcg taagtcacgt ggcgacggat tacacctcat ttgtacttgt agaggagggt
     6541 tccggagaat ctattgagtt ttatattgaa gtcggtgata tagtaaaatt ggatcgcgtg
     6601 gccatcagta ttgatctcga cgagaatcag cagaatacgt ttatggaagt agcccagatc
     6661 atcgaattgc tggaagcctt ggatcacggt gatcatattg cggtttcata tttcgacgtt
     6721 atcaaaggtc gcggtgtttc tgtcaaaggc atcatcacgc tcaaagatga atacaataag
     6781 gtgtttgctg tcgagtacga agaaaatgga gaaaagaagg ataaacattt taatctgaac
     6841 gaagataaaa tcatagatat tattatggag taatggtatt cggtttttcg gttgttgtct
     6901 tatgaaaatg ttttcaactt ccaatctaat aaccggttct tacggagact ttatttctga
     6961 aaacttacga tgcatgtaag ttttatatac ccgaatttaa cattcatttt tttgaataaa
     7021 ggaatatggc taaacgcgac tactacgaag tgctgagtgt ttccaaaaac gcttcgttgg
     7081 atgaaatcaa gaaatcgtac cgcaaattgg cgatgcaata ccatccggat aaaaatcccg
     7141 gggataagtc cgcagaagaa aaatttaaag agattgcgga agcgtattcc gtgttgagcg
     7201 atgaacaaaa gcgtgctcaa tatgatcgtt ttggtcatga cggtatgcgc ggcatgggtc
     7261 agcaaggatt cagcgacatc aatgatattt tttcacactt tagcgatatt ttcgaaggct
     7321 ttggtttcgg tgatgcattt ggcggtgggc gcgggcgcag tagccgttcc aaccgcggcg
     7381 ctgacctgga agttaagttg aaactgacac tgcaagagat cgccgaaggg gttactaaaa
     7441 agataaaact tcgtaaacgc aagtcctgtg aatcatgtaa cggaagcggt gcagccgccg
     7501 gatcatcgcg aaaaacgtgt agtgtgtgtc acggttcggg tcaggtaaga caagtttcgc
     7561 gatcggtatt cggccaattt gtgaatattg ctgcgtgccg caattgcgaa ggccagggaa
     7621 ctatcgttga aaaaccctgc tcaacctgca agggtgacgg agtgaccatg ggtgaaacgg
     7681 tgatcagcgt caaagttccc gccggtgtaa ctacaggaaa ttatattccg ctccgggggc
     7741 aaggtcatgc cgcaccgcat ggaggtgaat cgggcgatgt gattgtcgtt ctcgaagaaa
     7801 ttgaagatga aattttcgaa cggcacggtg atgatgtgtt gctcaacgta aatatcacgt
     7861 tccctcaagc ggttttaggg gatgaagtgg aagtgcctac cctatttgga aaagccaagt
     7921 taacgattcc gggaggtatt caatccggca aaatactgcg catgcgcggc aagggcattc
     7981 cgcatctcaa tggctccggt tccggagatc aattgattcg tgtcgccgta cacacaccat
     8041 ccaagatcag cgacaaagaa cgccgactta tcgaagaact tatgggttat gataatttta
     8101 gatcgccgaa tagtaacgac acgagtttct ttaaaaaaat aaaggaagca tttacggcat
     8161 aattatgcca ttgccggggg aattggggga tgaattttag gaactggggc gcaaacgcct
     8221 ataaattttt tgaatatttt ggtttgacac ggcaggaggc tgttgttctt tgtgtgctgg
     8281 cgggaataac tctgctgggt ggattagtag gtctgataaa agaaacgtat cgtgatgaca
     8341 cgatagagat tacatccgtt ccttatccca agaacgaggc ccgtccttat cctcagaagt
     8401 ctgaaaatga tagtgccttt tcagtatggt tttcacgcca agatacgttg caaacgttgt
     8461 ttcgcaaggg tgttgcacga gattcgatac aacggtttat attaaaaaca aacgacgcgc
     8521 ccgcacgagt ggttcgaatc aatcaagcgg atgtgcagga aataagttca cttcccggtg
     8581 tcggtcctaa gatagcggca cgtattatcg aataccggaa taaaaacggg ccgttttcta
     8641 agttggaaga tattcaaaat gtcaaaggtg tcgggaagaa actcattgaa aaaattaggc
     8701 cattcataga tattaactga gttatggcat ccattcctaa aacacgcatc acggattata
     8761 ttctcaaaac aattattgtt ttgctgtttg tttttttagt gtggagtgtg gtttcgcccg
     8821 tgcaagccga gaaagcggat cgaaataaaa ttcgattaac acgtgctaaa atgaaggctt
     8881 tgttggcggt acaaaaagca tattttgttt ccgactcctc atttacatcc gataacggca
     8941 aattattcga tttcgttaat aaaaaaggaa aagacaaggt tccggacgaa gtttttactc
     9001 cggtgattga ggccttgatg cgatttcccg agtatagtga gtacttcaaa gatgttacgc
     9061 ccgcgacgta ccgtgaacag tattttgaat ctttgactga taatccgatg aacgggcaaa
     9121 aatttataat agaaaccggt ttgaaagatg gccgcaagac ggtccatata aaaccatcgt
     9181 ccgtggattc agaaattcag cgaatcgggg ccgtcataga tagtcgtgtt acttggaatg
     9241 aaaaactaga acttacttac tgagatagcg ttagtattat gtcacaaatg agtatacaaa
     9301 gcgatcgcgg cgatctggat catgatgcca tagatcaaga ttatgcacgt cgagcaggtg
     9361 aatatcgtaa cctcgcgttt ggcctgagtt ttcacggaca acagattcat tttgcggaaa
     9421 tactacgttc ggaagatggt ttttcggtca gaaaaatggg caccattcag acgactatga
     9481 agtttggtac cggcattgaa ggtgttgaac gtaacgtaca aggtcttttt gcgtacctca
     9541 aaggctgcct tgaggataat ggtattcgcg ccaaacggat gaatatcagt ttaaatacca
     9601 atcttgtaac cttacataag gttctggttg agagtgagcg ttcggagtca gattttcagg
     9661 attatatcaa atgggaattc cgtcagcaag ttacggacga ttttgatcaa tatattttga
     9721 atacggttcc gcttgagaag acatcgccgg caccgatgga tcctatgctg atggtgggta
     9781 tccgtcgtcg ttatgccgat agtttgcgtt cggtgcttga taaaagtaaa attcagttca
     9841 gtaacatgga tgtggatata ttatgcacgc atgcgaccta tgaagtgaat tacgatattt
     9901 catatcacgg tatgaccgtg ctggccgaat gcaagaccgg ttctgtgact ttacttctgt
     9961 gcaaagatta cgatgtgcag cacgtatatc agtttaccgt ttcacccaaa agtaccccgc
    10021 aaaaaatagg cgagcttatc aatgcgcata tacccaatat gatgcaggca tatcatgcaa
    10081 cggcgggtaa taccaactcc gtcgtaggac gtttgatatt atgtcatgac cttgcgcaag
    10141 aagtattgcc atacatagaa gcagatttta atccatcggt aattgagcct tttcgtaaaa
    10201 taaaactccc ggctgaattc cggccgcatg aaaaaaatga agactccgaa tctgcatctg
    10261 cggatgccga cgaaaaagat aataaatcgc aagttgtcat agcgcctccg gattatgcgg
    10321 tgtatgcgga agcggtagga gcggccatca aacttcttat ggattgatat gatcaagata
    10381 aatctgcttg aaaaccattt tgcgccgcct gctcagcacg aaaaaacgga ttccgatacg
    10441 tcggccggca aaacgataac gatcgaggcc aaaaaaagcc aacgacggct tttgtttatt
    10501 attttgattt ttgtcgcgct tgttggcggt ttgacatacg ggtggatgga acgttacgcc
    10561 gttgtggcgt ggttagagca atatacgggg cctttgcacc ttttgccgga gcaagatatg
    10621 gagacggcgg caaaagcggc cgaagaagcg cgccaggaaa aaatccgaaa tctgtatatg
    10681 tacaacacga ttcgctatca ggagcggtac cgccgctttt tatttaggac ggattcgata
    10741 gtgtctgcga attcgcaaaa atggcatctc acttcgctcg gtgtggaaga aagtaactat
    10801 gcttttgacg tgtttggcaa aacacagaaa gatctcgaag attttaccgt tttgttttta
    10861 gaatcaaaaa gcatagacga aaccaaaccg caagcgatca aaccgacatc gttagtcaaa
    10921 ggtttgggat tcaaacgtac gatgtccggt acactcaaaa cggtttcgac gccgagtgat
    10981 tcggagatag tcgcgttaaa aataatctat cttgatttgg aaaaagccaa acaaaaaatc
    11041 ctcgacggtg cccgcaatgc cggattgaaa atattacaag acggtaaaat gacatcttcc
    11101 aaaggggtag tcatgagtac gcacagcggt gagttaaaac ttgaagggtc ggtttcggat
    11161 tacttcaaat acatggcaaa gctgaatgaa atgcgtttca acttcgaaat cggtgagatt
    11221 gatatacaat atgctgatta ttcggcccgc cgaaaggccg ataagaagaa actggtgccg
    11281 gatactattt ttatgaagta tagtattcta ctgcctcgcc aatcggaatc agccgactca
    11341 acgaagacaa aataattacc aatgcggttt taatgaaaat ccaatagccg aggtggccct
    11401 tcggctttat tttttatatg cgaattatag ccggtatccg acgcggtttg gttctgaatg
    11461 cattcgatga agagattatt cgtccgacca aagaccgagt taaagaatct gtttttaata
    11521 ttttggcaaa catcatagat gttaccgata cgcagatttt ggatttgttt gcagggactg
    11581 gcaatctcgg aatcgaagcg ctgagtcgcg gcgcggcaaa gtctactttt gtggaacttc
    11641 attccaaggc cgtacacgtg ttacgaaaca atgtaataaa atgccggttt gagcaacaaa
    11701 ttgatgtaat caccggcgat gcgttagagt tcatatcgtc cgttgccgta aaaaagtatg
    11761 atattgtttt tgctgatccg ccgtatgcga tgcgaattgg agctatgata gctaaaaaaa
    11821 tagatgccgg acaatggtta aatcctggag gcgttttagt aatcgaaacc tcaccggaag
    11881 aaattgaaaa tgagtggtta cctgcgcatg gattaacgat gcataagcaa aaaacctggg
    11941 gcgaaagtgc cgtgagtatt ttaatgaata aagcgaatta aatatgagac gtgcgattta
    12001 tccggggaca ttcgatcctc tcacgttggg gcatcttgat gtgatcgcaa gagcagcaac
    12061 attgtttgac gagattattg tcgcggttgc caataattca aaaaagaagc cgttgttttc
    12121 accggaagaa cgcgtagcga tgatcgccga ggcaacaaaa gatatggcca atgtgcgatg
    12181 cgagccatcg gaaggattga tcgttgactt cgcggtcagt cgccaggtgc acgtgcttat
    12241 acgaggcctt cgtgtgattt cggattttga atatgagctg caaatggagc acgtgaaccg
    12301 ccgcctcaat tcgcaaatag ttacgatgtt tcttatgccc ggcgaaaaat atgcatatct
    12361 gaattcgact attgtaaaag aggtagcgca atacggaggc gatttatcga gttttgtccc
    12421 ggcttttata gaacaacgaa ttaaagaaaa actgaaagtg caaaagtagc atgtccattt
    12481 ttggcaaaaa agaagaacct aaaaaaaata atccggtgcc cggaaaaccc ctaaaagaaa
    12541 tgacctttac cgagcgactt gtggaaaatg tcaaatcgtt tggttgggcg ctatgcgttt
    12601 actatatcac gatcaccttt gttgtacaaa acttcactat tccaacaggt tcgatggaag
    12661 tgtcgctctt gcccggggat tttatttttg caaataaatt tatttatggc gccaaatcgc
    12721 cggaaaatat tcccttcacg gatatcaaat tacctcagtt tcaacttcct gcgtttaaag
    12781 aaccgcaaca aggtgatgtc gtggtttttc gtttccccca ccctgaaatg atgccgagtc
    12841 agaacggatt agattatatc aagcgttgtg tagcggcgcc cggtcaaacg ttgtatgtac
    12901 gaaacaaagt tttgtttgtt gatggtgagg cattatctgt aaaatttgac ctaccgggtg
    12961 ttcactttga tccttacgag cgttccgaag ggcaagtttt tccgcgccac atggggagcg
    13021 gagataattt tggtccgttt cgcgttccga aaaaaggtga ccaaattgaa ttgaatctga
    13081 caaattatga attggtccgg tatctttcat tgcgggacgg aaaaaaactt gagatgcgcg
    13141 gcggggaatg gactgttgat ggcgttaagt caacgtatta tacagtcgga caggattatt
    13201 tttttatgat gggtgacaat cgcgataaca gtttggacag tcgctattgg gggccggtgc
    13261 cccgtagcca cattgcaggt caaggtatgt tggtttattg gtccaatacg gatgccgcta
    13321 aacaatcgtc cattccgatg aaagtactta tgatgttcaa ttttttctct gtcaaatggc
    13381 atcgtatcgg aacgattatt cgctaaaaaa atgcaagaac cggccggaca tatggacatg
    13441 acatcgtcat ccgacgtatc ttcagcggga gaatctaacg ctgtggaaaa accattgtca
    13501 gacaatcata ataaaacaac cgagcttttc cagattgcat tcgtttcggt ttttatcgcg
    13561 attatcgttc gtctcttctt tgtgcaagct tttaaaatac cgaccggcag tatggaggat
    13621 acccttctcg tcggtgatct cattttagtc aataagtgta tttatggtgt aaagacgcct
    13681 gcgacaatac ctttgatttt taccgacata gatttggcgc agtatcagtt tccggctgtg
    13741 cgtgaaccca aacaagggga tgtgattgtt tttaagtttc cgcctgatcc gacagtggac
    13801 tatatcaagc ggcttgtggc attacccggt caaacagtag agatcaaaga caaaatcgtg
    13861 tatgtggacg gcaaagattt ttcggaaatt atccggccgc cgggtttaaa atttgaggat
    13921 ccggaaatta tacctcgcaa taaaggatac gaatctgttt ttccgcgcga tgccggtagc
    13981 cgtgacaact atgggccggt cactgtgccg gaagattcat attttgtttt aggggacaac
    14041 cgcgatcgga gttatgacag ccgccactgg ggatttgttc ccaaagatca tcttatcgga
    14101 caagccgtaa tgatttactg gtccacggca agtcaggata attttgatat tcgttgggat
    14161 cgtttgggtc agatgattca ttaaatcact tcatgtttcc tgcagacttc gccacaaata
    14221 aaagacagca tacgcttcgc atccttgcca caattttgat tttttctcaa tctgtatgag
    14281 tgtgggtttc tttttcattt gataaatatg aagtacggcc tgatgtaatc cggcatcccc
    14341 gataggaaaa gcattggctt gacgaagaca cttcatcgcc acataatgcg ctgtccacgc
    14401 gccgatgccg cgttcggccg taagccgctc aactatttgc gaaagcgagt aattttgcaa
    14461 agccgttttc tcgattcttt tgtcggcgat ttgccgcgcg atatgaataa gatattctgc
    14521 tttacgcgtt gaaaattgta actttcgtaa cgtatctacc ctcaaatcag ccacggtggc
    14581 cggatctgga aataaaccat agggtaatcc gtcatgagta agcgattgcc cggcaacatc
    14641 aatcaaacgt tgttttaacg tataggcaaa agccagattg atttgttggc caatgacagc
    14701 ccaagctaaa gcctcaaaaa gatcgggaat accgatcaaa cgtaaaccgt gaaaacgttt
    14761 ggtaagtgcg aataatttgg gatctttttt cataagacga taaacgttcg ccagatcgca
    14821 tgacaggtca aaccattcgg aaacttctga acgaataaaa cgcgccgctt cgttggagat
    14881 gtctctttca aaacggagca tcattttatc cgatttaacg gttattgaag ttaagcaagt
    14941 ttcgtggcct atacgaaaag ctttagttag ggatttgttg tgaacgcgat gaagtatttc
    15001 ccgatcagac cgattcagaa aaacaaggca ttcgtcaaac gaaaagtcgg gcggaagttg
    15061 tatgtaggat tttaacatgc gataatatgt gctattcaat ttctgaaaac cacacgattc
    15121 ttgcgaaatt gaaatggaat aacgagattt ttaaaaaaag gaggacttgt gagagttcat
    15181 acacactacc tttggtttaa cactaaacaa agaagagaat atatcaatat caccgatgaa
    15241 gtagagtcgg cgcttaacgt ttcggggatc aaagaaggca tggcgcttgt gtcggcgatg
    15301 catattacag cggcggtata tgtcaatgat gcagaagatg gtcttatcaa ggacattgat
    15361 aaatggctgg atcatctggc gcccgctaat ccgaattacc ttcatcatcg taccggcgaa
    15421 gacaatgcgg atgcgcattt aaagaacttg ctattgcatc atgaggtgat tgtgccggtg
    15481 accggtggtc ggctggattt cggtccttgg cagcagatct attatgccga gtttgacggg
    15541 caacgaaaaa aacgcgttgt gatcaaaatc atgggcctat gatcatggaa aaacattgca
    15601 aaataaggtg attttgatta gtattgacca gtaaaaaaac aagctcaagg agaatataca
    15661 atggctttta cacttccctc gcttccgtac gcacatgatg cgttggaacc tcattttgat
    15721 aagatgacta tggaaatcca ccatggcaaa caccacaatg cctatgtgac caatctcaat
    15781 aacgcggtgg ccggtaagcc tgaagaaaac ctgagtatcg aagaaatctg caaaaatatt
    15841 tccaaatatc ctgtcgccgt gcgcaacaat ggcggcgggc attttaatca ctcgctcttt
    15901 tggacggtgc ttggccccaa tgccggcggt gaaccgacgg gcgcattagc ggatgcgatc
    15961 caatcggcat tcggttcttt tgccgatttc aaaactcaat ttgcaaatgc cggtgcgaca
    16021 cgctttggtt ccggttgggc atggctcatc gtaaaagaag gcaaattggc gatatgctct
    16081 acccccaatc aagataatcc gttgatggat attgccgaag ttaaaggtgc gcctatatta
    16141 ggattagacg tatgggaaca tgcatactat cttaaatatc aaaaccgtcg tccggattat
    16201 atcaccgcgt tttggaatgt ggttaactgg aacaaagttg ccgaaaactt cgcgaacgcc
    16261 aaataagttt tagattttcg aaaaaaggaa acatccttta tggatctcac gaaagaactc
    16321 aaagataatg tgcttctgac gaccgtcgaa gccgtttatg actggggccg ccgcaattca
    16381 ctttggccta tgcctttcgg tacggcatgc tgtgcgatcg aaatgatggc gacgctcgga
    16441 ccgcgctatg atatgtcgcg attcgggtcg gaagctatcc gtttttcacc gcgtcaggct
    16501 gatttgatga ttgtttccgg ccgcgtctcg atcaagatga tgcctgtgct caaacgaatt
    16561 tgggatcaaa tgcctgaacc caaatggtgt atttcgatgg gcgcttgcgc ttcgaccgga
    16621 ggttttttta acacctacac gctcattcaa ggtgttgatc agtttatccc ggtggacgtg
    16681 tatgtaccgg ggtgcccacc acgtcccgaa ggcttgatcg aaggtgtgat gaaaattcaa
    16741 aaacttattg ccaaacgcga agcggcttac gcacgcgaca tcaaagatga cggtatgtat
    16801 ggcgatgata aagtcggtat cgcaggtcgc ggtaaagatt tagtagcaga aattgcgaca
    16861 ggaaaattct gaaatttaca atcttagcaa taaaaaagct gcttcaaatg aagcagcttt
    16921 ttttgtctcg aaaatacaat ttgttctagt agcttcgcga ggcttctttc atgctgagtt
    16981 gaacgcgtcc ctttttgaga tcaacgctca ttacttttac atccaccaca tcgccgacag
    17041 ccaccacctc catcgggttt ttaacatatt tattggataa atggctgata tgtaccaaac
    17101 cgtcctgctt aaccccgata tccacgaaag cgccaaaatc aacgacattg cgaactgtac
    17161 ctttgagcat cataccttcg cgcaggtctt ccatttttaa tacatcggat ttgaaaatgg
    17221 gtttcggcat ttcttcgcgc ggatcgcgac ccggtttttt aagattatct aaaatgtctt
    17281 tgagtgtagg aacacccaca ccgacttccc cggctatatt ttcaaaagac atttttgcat
    17341 tacgcagttg cagctcaagc aggcttccgg ctgttgacgg atcgacgata ttaaattttt
    17401 tgagcagctt ttgtgtggcg tcatacgatt ccgggtgaat ggatgtatta tccagcggat
    17461 tatcgccgtc cggaatacgt aaaaaccctg cgcattgctg atatgcggcc ggaccgatac
    17521 cggtcacatc cataagctgt tcacggcgcg agaattttcc atttttatca cgatgtgcga
    17581 tgatggattg ggctgttttg gatgagaggc cggatacgta cgtcagaaga gcagcggagg
    17641 ctgtgttaag attaacgccg acatagttta cagcggattc cacgacatgt gtaagctgtt
    17701 cggccaaacg tttttgattg acatcatgtt gatactggcc gacgccgatg gatttaggat
    17761 caattttgac caattcggcc aagggatcca gaagtcgccg tgcgatggaa atattgcccc
    17821 gttgtgaggc ttcaagatca ggaaattctt gttgtgcgat ttttgaagcc gaataaaccg
    17881 aagccccggc ttccgatacg atcgtataca caagttccag atgagaatct tcttttttga
    17941 gatcggcgat cagatcggcg acgagagatt ccgtttcgcg ggacgcggtg ccgttcccga
    18001 ttgcaatgac gctcacttca tattttctga tgaattgttt caaaacgcgc tttgagccca
    18061 aagcgtcttt ttgcggttca tgcggataga tggtttcgcc ctcaaggtat tttccggttt
    18121 catcaatgac agcgactttg catcccgtcc taaatccggg atcaatgccc atgatgattt
    18181 tgcctttggt gggggcttgg agaagtaggt ttcgcgcatt acttccgaat acttcgatcg
    18241 catgttcttc ggacttttcg gtaaactcgc cgcggatttc acgttcaatg gaaggcgcga
    18301 tcaagcggaa ataactttct ttgatggctt cgataacctg atcgcgaaaa atggattttt
    18361 gttttttaac gtggcgatat ccgatgatgt tacaacaagc ttcttcgtca accgtgattt
    18421 tgatgcggag tttttcttcg cgctcaccgc ggttggtagc caagatacga tgcggaggca
    18481 ttttggcgat cggctcgctg aatgtatgat acatttcata ttcgttttcg gggtcttctt
    18541 tcacttcaga cacgaggatg ccgttctcac gtgtaaattc gcgcacatct tttcgtacat
    18601 ccgcatcttc gctgatgatc tcggcgatga tatcgcgtgc gccttgcaac gcttcgtcga
    18661 cggttgtaac ccctttttcg agattgatga agggttgggc atgttcgtct agggtgccgc
    18721 ttgtaatctc ctgtgcgagg atttgtaaag cgagcggttc gaggcctttt tctttggcga
    18781 taataccttt ggtacgacgt tttggtttat acggcagata caaatcttcg acttcttgaa
    18841 gcattgtagc ggctacgata cgcgctttga gttcgtcggt aagtttacct tgctcttcga
    18901 tagacttgag tacgacttct ttacgtgctt ccaatgcgcg atagtatgtc atcttatcct
    18961 gtatatcgcg gatttgattt tcgtccatgc cgccggttac ttcttttcgg tagcgtgcga
    19021 taaaaggaac ggtgttttcc aggtcaagca actgaatggt tgaagtgacg tgttggacgg
    19081 gaatgttgag ttctttggca acgagacgca gaatttcctg ttcggacatg cgatcaaaac
    19141 tcctgattaa gaaatggaaa atacgcctga tagttttaaa atgattattt tcggcgggca
    19201 ataatacgag agtgaaagaa gaaattcaag gcggtatcaa tcgaatgtaa agttttcgat
    19261 cagatgcttc ctaatgggtc ggttacaatg cgaatgcgta gaataattta atgtgaaggc
    19321 aattcatgaa aagttttaaa acggcaaata gtttccgttt gcatcaattt tatatatcaa
    19381 cgttttcgaa taataggaag aatttatata catagccccc ataatcaaat aagtaccatc
    19441 gccgtttgcg tgaaaagaga caagagaaaa attattttca tcgggtgcta aatgtgattc
    19501 tacccaggag ccgtcgcttt gaagccgcat gagcaaaatt ttattgctac caccccatcg
    19561 acctaataaa accaattcat tcgctccgag gctttcaaaa tcggtaaaat ctgtactcca
    19621 gtttaacgga atagtatcca gcggggttat agcgccgtca atatccatcg cgtcaatggc
    19681 taggcgatca gggatataat tgtatggatt atatagaatt ctggcgatgc gatagtcact
    19741 cgaagaaacc ctgcataaca agaatccctt tggcaacata agactttgtt tccaaagtac
    19801 gtcgccgttt gcatctattg atacaatgct gtcattattc cactttccat agatcctatt
    19861 attggcgaca tgcaaatatt cgaaagaaac cggcatcact ttttcccact taagttcgcc
    19921 atcgttatca attttactta gttttgtttt aaattgtaat gagtcaaaaa tagatgatcg
    19981 cactaagtat tcgccgttat tcatcggaaa tatggaagcg tactttattt gcacaagctc
    20041 tttttgattg gttatgtttc cgttagtatc aagttgaata aaaagggaat tttggcctgt
    20101 gtccttgggg tttattacaa gtgtaaatat gccgttacta taacttaaat ttctttcaaa
    20161 atatgacgat tcaatatagt gtgtagccgg taaaaatttt tgaaacttct ggattccgtt
    20221 gggtgtaaac cccgtcaaac catagcgatt tgtgtcatag tgtaaactga gaatggcaat
    20281 gttattctct ccgtattgaa tcatatcctt tagaattaca gtcgcagaac ctgggtccca
    20341 ttcaaagtgc ggtggttgtt tatcgccgga agatgtatcc ataccttcgc tgcatgccgt
    20401 caaacaaaca cacagacaca tcagaattgt attgtaattt aaaactttca tcgttgcttc
    20461 catttggatt ggacatggac ggttatcggt cgtttatgcc ttgtggcggc gctaaaatac
    20521 ccgtatccgc cgcggatgtt agatttgtgg ttataatacg tatagtcaat gaaatttcgg
    20581 tctatggcgg taatatggag ttgtgatgta taaacgaact ccctttccga atcgatataa
    20641 aaaccactcc atgtccaagg gttatccggc ttattataaa ggccccgata agttgtagaa
    20701 tcccagtgat caaataatac tagcgatgaa tacgcgatcc atttttcggt ctgaaataat
    20761 tttgagtttg gtatgaaaaa ttcgcgatcg gattctttaa gataagaagt gtcagcgcca
    20821 atctcccaat aataggcttc agctccggtt gcatcactcc agtcaaacga tactgtcgga
    20881 gaacgaagat aatacatctg tgtcaaaacc ggatacggat atccggaaac tattcggcga
    20941 accgtatcca actctatcgc tacgctaaaa ctatccggcg tcgtctcagg gtcaggaatc
    21001 ggcacgcccg gaagtacaca ctgcgcatga acgcttaatc cgtccttggc ttttacctca
    21061 agtttataaa tcaaccccgg ataatatttt aacattccgg ctgtatccac atagcggccg
    21121 ggtattactt ctgaaagcgt gacgtgttgc gaatcgctgc taataataac ttcagcgtta
    21181 gattctttta tctgaagcgc ttcgggtata aactcatcgt agttttcctg gctgtaagta
    21241 cgcatgagaa tgatcgtcgc attttcggat gcatacggca tcaaaattcc gaaaacgcct
    21301 aaaaactcat tattattatt ttggttacag gcgctgccta cgaaaagcgc caccatcaca
    21361 aatgtcattg attttttttt cacctttaaa actcaaaaaa agttccgaaa cttggtaaaa
    21421 taggaacgcc gtatcctttt cgtttactgt agttcatacg ttggggatca ggttcatttg
    21481 agattatact accattgtag ttgtaatcgt aaaacaaagg attattagaa tagtaggtat
    21541 ttattatgga aatataaggt ttcattgtcc aggttttgaa acgaacggtt ttgaccaagc
    21601 tcacatcaag ccgatggtac agcgggtaac gatcataaaa ctctaagtga tccaaatttt
    21661 cttgactgat attataacca ttatcttcca taagcgacct gccgctgccg ggttcaatat
    21721 atgctgttgc gccataaaac tccgtaggtc ggtaggcgcg acctgaagcc agaatccatc
    21781 gtgcatccac agtccagttt tttggcaagc gataactgag gagtaaattt aatgtatgac
    21841 gctggtcata attagtgaca tatgtatttt ggtcaaatgt gcgtgtaacc caggctaaag
    21901 aataatcggc ctcaaaactc attttattac ctattcgacg gtaagtgatt tctaatccgt
    21961 atgcgtcacc gcctccttta tcaaagtgaa ggctatctga tgagtggtcc tttcggtaaa
    22021 ggcgattaaa atgtttgaaa tagatatccg ccgtcaatct aatgtttgga tttggaaaaa
    22081 attctccacc tgcaatgatg tgagtcgctt tttcaacctg atcttcagtg gagaaaggca
    22141 cttctaaaaa accgatatag ccgcgctcgc gcgcagtaaa aagagactga taatatatgc
    22201 cggcgcttcc tttgagcgct agtttgggcg agacatcata ccgaatcgcc gtacgcgggc
    22261 ttatcaatag agatcgtttg ttgcttgaaa aatgttctat tcgaataccc ggtttgatcg
    22321 tccagtcacc ttttttccac atgtcttcga tataaaatgc gagattagtt gtgcgacgct
    22381 gataacgaaa gctgtccggc gcaaaatcgt aaaacacttg tacataatgc ggtggtgcgt
    22441 taaagttttt ccagtaatag gacatttcga tcaagttaag tgaaacgccc agatacatac
    22501 tgtgttcatt taaccattcc cattgtgtat cccatgcgaa ccctcgatct acgatatagt
    22561 ttcgcgtatc aaaaggacgc ttttcctgta atgcattaag gctatctgtc cgccgacgga
    22621 tcgaatctgg cgcaccgctg tcatacccgt aatagaggtc ttcatccagt ttgatcgtag
    22681 ctttggattg gctgatcgta acgcgcgatc ggaaaatatc tcctgcctgc cagtcccaat
    22741 atacggcagc atttaaattt ccccagccat agtcaaggcg gccgacgcta tacataggta
    22801 actttcgact aatgttaaat tcgttgaaga catattcata atccctgtgc tttgaaaaaa
    22861 atttatcccg actgaaaaac aatgaagtac caaattcgtt cagtgtattc ggacggtaca
    22921 ctactttact tagtccgtca taaaaagaat aaggaattga ttctggagct atttttaacc
    22981 ataccgccgc tttggtggca aaatctaaat aggtccgacg caatgaaatg aggtaatacc
    23041 aatgtttgct gggttgacca tccatcgtga ttttggaact gagcaagctg gcgttacccg
    23101 tggccgagaa cttatttggt gaagccgatt tagtgcgtac atcaattaca gagctcagac
    23161 gattgccata ttgcgctgaa aaaccgccgg tggaaaaatt cacttgttcg attgcgtcag
    23221 tattaaatat gccggctata ccgaaaaggt gatacggttg atatacgata gcacgatcca
    23281 ttgtgattag attttgatcc ggagttccgc cgcgtacata cagttcgttg gatagatcgt
    23341 tgggagcggt gacgccgggt agtgtttgca aggttctaaa aagatcaggt tcaagaacac
    23401 tcggagttga gttgattttt cggcgatcta taacgatgat gctgggttga acgcgcaatt
    23461 tggcatcact gacacgccag gtttcccttt caccaaagac gatagtggag tccaatcgaa
    23521 tgtcgctggg ttcgagacgt atcgtatcca gagtgatttc ttgagatgaa agatgcaacg
    23581 ttttgtccaa aggcaaataa cctaatgctg ttatacgtag tgtgtaatcc ccgtaaggaa
    23641 tgtgctgaac tacagcttgg ccatgtgcat cgctcaatgc cccgcgacga ctaccggaga
    23701 ttgttacgtt ggcaaaataa atcccctctg acgtacgtgc atcaacgatc gttaggcgta
    23761 cagatgtgaa ctgacctgaa gcttcttgct ttttggtgtc gtcttcatac aaaactataa
    23821 tattttttcc gatgcgtttg tatgcgagtg gtttaccgat caatatatac tctaaggatt
    23881 gttgcaaatc atcgcctttg atcggagtac ggacagtata ttttgctatg gtctggtcaa
    23941 catatgaaaa tcgcacatgg taccgtgact ccaattctgc gacggcttgt ttgagagata
    24001 tggcactttg agcagaggaa gaagcaatct ccttctcctg agcaaaagtt agataagtcg
    24061 tgaaaaaaaa agctacaaaa aagacccgat agaagttcat gaagtaataa aattcatttt
    24121 tttagtttta agaaaaaagt agaatcattg acgattgtgg tttcaaaacc ggcggacaat
    24181 cctaattgtt ttaaagcgct aagactggat gaaccgtgga tcaatccggt gaaagtgcgt
    24241 ttggtatcta tgctatccat agtaatggtt agtccatagg tgattttcca atcgtttaag
    24301 attgaataaa tcggagctga ttgatagttc catattcctt gcgaccatcc tgcaactagc
    24361 gtcgtatcca tagaatacgg catggaaaaa tgtccctgat gcatacttac ggcgagaccc
    24421 ggtgtcaaaa ttgtattttc tgagttgttc cttgcgctta cgcgtacctt tccggatgaa
    24481 cagacaacct ctgaacggtc tgcacgatcc cttacattaa atttggttcc taaaacctcc
    24541 acattcaaat tttctgcagt tacattaaaa ggccgtttca ttttttgcac ttcgaaatac
    24601 acttcgccat gcatagtaac gctgcgcgtt aacggccatc gccaggagtt atagcgaatt
    24661 gaagatagcc cattgagtct tgctatagag ccgtcggata atacgatttc ggtttgttgt
    24721 gcctttgaag ttgttatttc tctgtaagcg aattgagact ccaacccaaa gtaaatgaca
    24781 atgcttgcgg ctatggctgc agcccattgc gttgtgtttc tgatccaact tcgtgaaacc
    24841 tgtttggatg tagttgcttc agcgatagcg ctccacatgc gctcggttgg agcctttggg
    24901 atctctaagg atttttgtga ccaatgaatt ttcgttaatt cattgaaaaa ctcttgctga
    24961 tgggagttgc tttttagcca cagctcaaat tctttttttt cttcgtcggt caatgtacct
    25021 gcgatccatc ggccgataaa gtcatcttgg tgatagtagt tttcactcat ggttatttat
    25081 tttgtttgat atagatttct atactccaga aggcatcgtc ggattcgatt tgctttagtg
    25141 aatctccgca atagaatatt ccaatgaatt gacttcgcca cgatcgtcag cataaaatcg
    25201 gcctccgtcg gagtaccaat gcatcatgtc ttcaccgtct tcgtaacgct gattaaaaaa
    25261 agtaccgttt gcctaaaacg taattatgga tgaacctccg gatagaccaa ggcgttcgat
    25321 ttctgtaggt acactatcta tgctgtcttc gccgtatcgg gattcttgcc aaattcatgt
    25381 tcccaaaaaa atgcggcatc gtgttctttt gtttctttat ctttacagct agccagtgca
    25441 aagaaagtgg atatatatat gcacgaaaga caatctctct tcttggtatc ctcctgatta
    25501 gtaaattaaa taacctccaa agactataca ctttaaaaag gaaaaaccct tagtcataag
    25561 taaaaaaata aataaaaatt aaagatttgt ttttaatgag gataagtgtc gggaaatggg
    25621 tcagtgtttt aatgtagtcc gcaattgctt caaagctcta cctatgtgtg cttcgacggt
    25681 tttaacacta atggaaaggc gttcggagat ttctgaatat gataagtcat catagcgact
    25741 catcatgaaa acgatacgtg cttgttcggg taatttttga atcgcagaat taagtagttg
    25801 ttgaaattct tttttctcca aaacagacag aggcgtttct tcgtgcggtt gggtatcagg
    25861 atcttcgatt ccctcaccgg tacgaattgt atgaccacgt atagtgttga gagtaagatt
    25921 aacggctatc gtgtagagat aggatttgat ggataaatct tctcgaagat tgaatcgatt
    25981 ttcccacaat cgaataaaaa catcctgcac aatatcttcg gatagtgccc tatcgtaggt
    26041 tttataaaac acaaaatgat acaacggacg atggaaaaaa tcgaataacg tacgaaaagc
    26101 cagagcatca gagttgcgta tttttttggt taagagttgg atttcctcgg tggtcatacc
    26161 cctatagagt tttcattgtt ttcatgaaac tagaataata atatagcgca acatgggaag
    26221 ggaggcaata aacaaaaggt tacagaattt tttgtcttac ccgtaatcta tattatttgt
    26281 attgcacagg catcgactag aaataaattc cgaccaatcc gccccaccaa gcacgaatcg
    26341 tggtatgctt ataatcccaa ttgtaggcat tcgtcggagc cggaaaaccg ttttcagcgc
    26401 gcgataccca tacattggct gatgctccgg catacacgga tagatgtttg tgtaatttaa
    26461 tcgtaggtgt gatttttaat gtgtttacca tgttgagccg gtgttcccaa aatttatctt
    26521 tattgtgttg gtgggtcgta gcctcgatag cgagtgataa acgccgatcg ggccaaacta
    26581 ttgcgcctat accgccgccc agagcccatt gtgcaggttc acgaagttgg cgaacagcta
    26641 catcgtatat cgtgtacata tatttgctgc cgtgagtata agaaaaatgg ttgaaaccgc
    26701 gctcggtgcg cgctaaacgt ccgtgatgtt gcccgttgcg aataaaattg agtaagccca
    26761 gcgcgatccc gttgttctcg tctgcaaaat taaacagtcc tatttgtgcg ccatggactt
    26821 gttttgcacg attgaccaat gcgatctgaa ttccgtgtac atgactggct tcattgaccg
    26881 aagcaagttg caatccgcgt acattttcgg catgattgta ccccgcgccg atttgtaaac
    26941 cggaatgatc ttttttggat atattgactg caccgatctg aagaccgttc gtttggtctg
    27001 caatgttgac ggtcgcgatt tgaacgccgg acaaatcacc tgtttgattg gaacctccgg
    27061 cgatctgtag accgcgtgcg tgaagatacg cgcgatttcc tcctccggcg atttgtaatc
    27121 cgttaacata catccggtcg taatttccga taagccctac ttcaacaccc tcaatgctta
    27181 caaggcgagt agaaatgatg ttgatcgaaa ttgcatgcag cggcacgggc acgtaagccg
    27241 gttcgatgcg tgtcgtggga gcgaaatcca tgcccaaaaa ttcactacga acgcttcggt
    27301 ttttcaagtc gtgcggctcc gttttttcag taggtacact ttgcgtttcg gtttgtggcg
    27361 acatgggtgt tggtatcacg atagaaggag aggtctcatt tttatcaatg gatacggtgg
    27421 tatcggtcgg taatgaatcg tcttcgtgtt cgccggattc ttgttcacca tccaaagttt
    27481 tattcatggt actcagcgct tcatcggcgc gtgatcttcc ggcttgcaaa cgcgcttgcg
    27541 ccgtagcttc aatgttgcta ttggtttttg tttttttaaa tagcgttatt tcttttgttc
    27601 cgataaaacg gtaggtgaga tccgtttgag caaaaacata atccaattgg tgattgagtt
    27661 tatcggattc ggaaggtgta ttgacgcgaa cattttgtac taaagccggt acataagaga
    27721 aaacaacatt atgttttttt tcaatacctt tcaatacatc gctgagcggt tttttatcac
    27781 catcgccggc ttgaagtgat gaaaaaacaa ataataaatt gattaaacat agcgttataa
    27841 attgacacat aactcccctc attttttgtt ctcttctaag tacaggtaac catctattca
    27901 tgacggatgt cttttgtaaa tcggaatttt tgcttaattt cgtctgacca taactatagg
    27961 gagttattta tgcggttact attatgcagt aattcgacta actttggtga aaattatcta
    28021 gatcactggg ctgcggagat cgttgatttt ttaggcgcga taaaacaaat tgtatttgta
    28081 ccatacgcat tagatgatct gaatgcatac acaaaaaaaa cgagggatag gtatgcggag
    28141 atgggaatac gtgtggccgg gattcacgaa aacgatccgc gtaaaatggt aaccgaagcc
    28201 gaagcgatac acatcggcgg agggaataca tttcggcttt taaaaaaatt atatgacgaa
    28261 gatttgattg atattattcg ccatcgtgta agcgacggca tgttgtatct tggtgcaagt
    28321 gccggtacca atgtggctac aatttccatc aagacgacca acgatatgcc gatcgtttat
    28381 ccgccgagtt ttgaagcact caatctggtg ccatttaata ttaatccgca ttacatagac
    28441 cctgatcctt cgacccaaca tatgggtgaa acccgtgacc agcgtatatc ggaatttcat
    28501 gaagtcaata atgctccagt gcttggtctc agggagggtg cactcattcg gcgtgagggt
    28561 gacaccttaa ccctcaaagg aaaaaacgga gcaaaattgt ttaccaaagg taatacgcct
    28621 caactcaata acacgggcgc cgacctcagc tttttattgc gaggtttttg ataaaatcat
    28681 caagatttta ctcaaagatg tttttggcat aagtattgtt tatataatat gtgtaagcat
    28741 atgagaatgt tcttcgaaat ttgtaaatta taaacttaag aataaaatag agttaaaata
    28801 attcagtttt ttgaatttta acttcagttt tttgaaattt ttctcctttt ttgggtttag
    28861 ggttcgttat tctgatttta gggtttcggg ataacgtgca agcgccacag ttagatgggt
    28921 tctgtggcgc tttttttttg cttattctca agccataatc atctcgtttg taaagctgtt
    28981 acttgcatct tgcctaaact aaaattattt tttaagtact acttagtata tcatttatta
    29041 catgaggata gattatgcta cgtcaaatta tattagtgat aacattgttt tctgcggttt
    29101 taatcgcgca ggataaaaaa attctgacat acgaagcttt tgttgatttg cggcgtgtgg
    29161 acgaaccggc tgtttcaccg gatggtaatt gggtcttgta caacgtgcgt aaatactcgc
    29221 tggaatccaa tacgtatact tccaatattt ttctgacatc gatagatggc aaaacttcgc
    29281 gccagttgac tttttcaccg accaaagata ttacgccgat ctggtcaccc gatggtaaaa
    29341 ctatagcttt tttatccaat cggcttggtg gttttcaaat atggactatg gcattggacg
    29401 gtggcgaacc aaaacgtgtg acgaatcttt ccacgcaggt ttcaggtaac ggattggtat
    29461 ggtcgcctaa cggccaatac attctcatta cgtcggatgt gtttccggat tgtgaaacgg
    29521 atgattgtaa tcgcgctcat aacgatgcgg ccgaaaacag caaagtcaaa gcgcagctca
    29581 ttggcaaatt gccattccgg gtttgggatt cgtttaaaga cggtaaac
//
LOCUS       DAOQNQ010000011        29555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_498_length_29555_cov_6.128949, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000011 DAOQNQ010000000
VERSION     DAOQNQ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 29555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29555
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_498_length_29555_cov_6.128949"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="PK754_01500"
     CDS             complement(<1..1111)
                     /locus_tag="PK754_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HNB08211.1"
                     /translation="MAHDAQAGSSTTGSHHFTPYIAPEHNVPEFTAKALILGALFGII
                     FGAATVYLALKAGLTVSASIPIAVLAISLGRKLFKTTILENNIIQTGGSAGESIAAGV
                     VFTLPGFLFLSAESMGPSYFNYWTIFVLAMFGGILGTLMMVPLRRPLIVQEHGVLPYP
                     EGTACASVLVAGEKGGDFSKTAYQGLGFAFIYAFFQKVFHFIAETPAWVTQQTNKFFP
                     SATVNGEITPEYMGVGYIIGPRIAGVLVAGGVLAWLGLIPLLASLVPAATIAEQLVKL
                     GYLPDILTAGGPGGWDPATQTFGSTATAVYRAYVRQIGAGAVAAGGFITLIKTLPTIV
                     SSFKGSVASLKNKQSEESVRRTERDLSFGTVIAGSL"
     gene            1329..1826
                     /locus_tag="PK754_01505"
     CDS             1329..1826
                     /locus_tag="PK754_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB08212.1"
                     /translation="MSQPLTKPVLLIVDDEVEILKVLQKSLEDDYDVLTASRAKAGLE
                     LLDERVSVVISDQRMPEMTGSEFLRHVRERRPEVVRIIMSGYSDMTALINSVNYGEIF
                     RYLNKPWDLNELLNTIELAVKRYDENRRQIELASENQRLAAENLRLTEDLHEKTTELN
                     RLRGK"
     gene            1848..3134
                     /locus_tag="PK754_01510"
     CDS             1848..3134
                     /locus_tag="PK754_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNB08213.1"
                     /translation="MLKTIPHLLISDAQGRFSDIPQIAMAGRSGKNTVPVAADTLIPL
                     PEGSQLYFLPQRKAMGYDRKTGELIVFDDAYAVAAYIPPSYTHYLMAAYETITGAPRL
                     PLFSFTAVGYYRGRYYVPAVHVDEDIKQLPTSFDDKKVQRAVDAWIVRHPNNRLLAHH
                     GHACAIEYGCPNAKNLFLGRWEAPVAVASACNANCVGCISFQPKESVPSPQNRLDFVP
                     TVEEIVEYAVPHLEKAEHAILSFGQGCEGEPLLRGKLIEDSIRAIRRHTKRGVIHINT
                     NGSKPDTLARLFDAGLDSVRVSMNSAQLDLYHRYYKPNNYTFDDVVRSLKVARDHKKF
                     ASINYFVFPGITDSQAEIRALMDLIADTKLHLIQWRNFNIDPDWYLNDVVGDYQSKAA
                     GVPSLMRRVREKFPHLLFGYMNKPAEVIRDAVDRST"
     gene            3426..4304
                     /locus_tag="PK754_01515"
     CDS             3426..4304
                     /locus_tag="PK754_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB08214.1"
                     /translation="MKKLLSCLILFYLSVPSAEGSERTLPYFVSTQWLADHINDSDLV
                     ILQTAFTRKEYAVGHIPGSRFLWFNWLAMDTPDLSTEMPDSVTAKNVLENLGISQKSK
                     IVVVFTRGSVTTTTRMLVALSYFGFDNQTAVLDGGLDVWKAEGRPITKDIPTVQKTAL
                     ALKLHPEIITNAEWVKSRLSSEKVKIIDARSKNFYAGNGGGILRTGHIEGARNIPFST
                     LTDSTNKILPVASLQKIIDDAGIQKGDTVVTYCHVGQQATLVYTVARMLGYEVLLYDG
                     CFEDWNVRDESYPVKK"
     gene            4308..4820
                     /locus_tag="PK754_01520"
     CDS             4308..4820
                     /locus_tag="PK754_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNB08215.1"
                     /translation="MKPMKPYMNPYVAGVGLGLVLLLAFFIVGRGLGASGALSSVLTS
                     TVNTVAPSHIQSNAFYSEYYNNGNPLKDWLVFEMAGVLIGGLLSGWFSGRLKFKVEKS
                     ESVSTCTRFIFAFAGGILMGLGSKLARGCTSGQALTGGALMSVGSWIFIASVFITAYL
                     VAKFFRRQWK"
     gene            4844..5404
                     /locus_tag="PK754_01525"
     CDS             4844..5404
                     /locus_tag="PK754_01525"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNB08216.1"
                     /translation="MISFEQSLIIAVFIGIGFGFFLEQAGFGSAVKLAQQFYLNDLSV
                     FKVMFSALVTAMLGLFWLNKLDLLDLSAIRVLPTYIYPQLAGGVVFGVGFIMGGYCPG
                     TCCVASVTGRMDGWIHFIGMMTGIFIFAEVYPFIESWYFSTSLGDITLFQFFQIPYGT
                     AVFIVVMMALLGFVAAERIEKLHAKD"
     gene            5409..6029
                     /locus_tag="PK754_01530"
     CDS             5409..6029
                     /locus_tag="PK754_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB08217.1"
                     /translation="MTVNRFLFAICFVFGLLALLSGNSDPYHDKINEIADLVETENDH
                     VLPDSLAVWLMNEKPMRIIHLGDSSSYSRYHIPGAELKSLPDLLHSTLKKNELIILYS
                     EGGIHAGQAWVLLKAKNFKNVYTMHGGLSAWRSEILFPRLSPDAKDAEKKRFEERSKI
                     SLYFGGQPTVILEPKPEVTMKKQEPPRKKIDTPRFEKEEEKIRDGC"
     gene            complement(6032..6226)
                     /locus_tag="PK754_01535"
     CDS             complement(6032..6226)
                     /locus_tag="PK754_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08218.1"
                     /translation="MATFFIWCILLILCWPLALVALIMYPFVWLILLPFRIAGLAVEG
                     VLKFIWALFRLPFRVLSSGK"
     gene            complement(6304..7443)
                     /locus_tag="PK754_01540"
     CDS             complement(6304..7443)
                     /locus_tag="PK754_01540"
                     /inference="COORDINATES: protein motif:HMM:NF027610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4932 domain-containing protein"
                     /protein_id="HNB08219.1"
                     /translation="MNRFFGIVYMVTLFGLSETVFSQTPANELMLRVDPRVELLCIAF
                     RLAEYPEVIDTLNSAYTQRIDAHFQKHKNHPLIEDIRLLLDSVKKHDIEFGYWDIPAI
                     AIHIRAFADWTSVAKADPPVDDIWDNRTWLTPRRMELIRAFYYDSDARRFFDDERDYY
                     ARAEASVNHRTMHLQKTWLQKYFGMAESEAYFAILGLQLRQGAYLRVNTPHEMRQTFT
                     LFPCTAFDHWGLPVSLDSLQIIRSILHEYIHAFVNQAVDAYESQWQKPAELILADPHV
                     WSLMKDAFYNNPRYLVYESLVRATSIIYLTRHHPELISLEKEITTQEKWGFYWMGEMV
                     RLLEDYDKERNRYSDFYSIMPEVCRLLESVSGRMRDGKYVFPNHQ"
     gene            complement(7481..10102)
                     /locus_tag="PK754_01545"
     CDS             complement(7481..10102)
                     /locus_tag="PK754_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNB08220.1"
                     /translation="MIRSLIKKLWIGYVCLMWPATMTPLHTQDSATIITPERWARIHK
                     IDVQHIDLDLRLDWKTKSVEGYATIILQTRTVADRIELDGAFLTIHRIETGDGTPLRY
                     TYDGSDRDGALQIQLDRTLGTGEILTLTVFYKTGWVNYSDPNNLWGSNGKGIRFFEPT
                     FSEPRRRKQAWSISEYTSNRYWFPGHDTPSDLRTTTFSMRVDTPFTVIANGALLRTED
                     HPDGTRTFHWRMDTPYANHRTSFVAGRYSIFRQMTDGVVINNYSYPDEYDATVATVER
                     LPDMMRFYSEFTGQSYPFSQYAQVFVQDLPWGMENTTASTLTENMVDDHPTHTEFLFL
                     WDMLEGEALAMQWFGSYVPAASWEDIWLNRAFARYLSCMYDEYKNGRDEFLLWQLAFQ
                     HNTYLSDWASGIRLPVVARRTADEASLVTGNHTYLHGASVLHMLRKHVGDTAWKKILQ
                     HYVLTYGGRPVQTEDLRRSVEAVTGEPMDWFFDQWLYRTGHPVFTVTKEYHPGEKALT
                     LTVRQIQSPDTIASVFSQNRWFQGKMEIAVDDRIETIWIKPQSENVFRLACERAPRRV
                     IFDYEGTWIKEATFEKTFSEWIDQFTGDGDILARWDAMTRLSAMASDSGTSLSDKQNI
                     YAAFRTVIGGTAYWRLRYMATLQLQSLIAPPSNPKLVLDNETRRLLLTVIRRDSMWCK
                     TAAISLLGSTRDPSNASLFIDLLRDPKDRVVNAAAIALGKTKHSKAFDALSKLTRRPS
                     WKNQSLISALNGLRELGDARGADIAVAALSDRYAPRWTLVTPVWDFRIAAAQTLVALN
                     QSAKAYPIIVDRFQKAMEENDINDILNNALLIATLADPRGKEVFDALKAKFKNDANTM
                     TAVTQYENQFLEALKKK"
     gene            complement(10116..10703)
                     /locus_tag="PK754_01550"
     CDS             complement(10116..10703)
                     /locus_tag="PK754_01550"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNB08221.1"
                     /translation="MKKNVGILIFDDAEVLDFAGPFEVFAIASEVHDFSLYDVFTVAR
                     HMKPVRAVNGLLVTPNYDFSNHPPIDILIVSGGQGTRALLRDQEHHDWLQRVHRTTEY
                     TLSICSAARIFGALGLLDRKPYCTHHQVYDHLKEIVPTGQPQNDRRFVQTDERLYTSG
                     GISAGIDLSFHILEQRHGKEVAVRTAEYMEYRYTP"
     gene            10978..11901
                     /locus_tag="PK754_01555"
     CDS             10978..11901
                     /locus_tag="PK754_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNB08222.1"
                     /translation="MASQKIVFLILPEVHLLDLGGPEQVFLEAIGYGAPFEIEYCTYT
                     KELRSSGGLTFGHIKHFSEVAIKPEDYIFIPGPNVRSIHDYKFTSQTELLDWLRRCYR
                     ENITLCSVCSGAFALAYAGLLDYKKCTTHFKRTAELKRYFPKLDVQENVLFVEDGNII
                     TSAGIASGIDMALYILERIQGSHFAHKVAREMVIYTRRTGSQPQKSSYLDYRNHIHSG
                     IHKVQDYLSENIHKKSSVGHLAEIACMSERNFTRIFKKEAGITVNEYTTLVRKEKIKL
                     LLKNPDLSRVQIARAVGLNSDRQLSRIMHSI"
     gene            12001..12525
                     /locus_tag="PK754_01560"
     CDS             12001..12525
                     /locus_tag="PK754_01560"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB08223.1"
                     /translation="MDISVRRATIADATALALLARVTFEDAFGYVWTDKPVLKEYLDT
                     TFSVPKITSSLSKTNNSFWIALADGLPVGYAKMKKVSPYKLLEDNKPAQLQKIYMLRN
                     FIGQRIGERLQSALFDEVRMLGIRTLWLAVWDGNDKAIRFYERHGFQKTTRYAYDFHS
                     MHFEYEVMTKRFDV"
     gene            12522..12971
                     /locus_tag="PK754_01565"
     CDS             12522..12971
                     /locus_tag="PK754_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNB08224.1"
                     /translation="MKWWAASVPFGLAFVAAIGNALVTYAQKKATPFDHPFYFGAFSL
                     LLASLGLFGIATFFSSGKIIPYAVENFVWFAVAAAGLILLNIFLYVLYRHYGAAYYTL
                     YAILAMVTTSIGLAVLVFKESMNVYFWLSLLFAALTVVCFIKGKSGG"
     gene            13045..13794
                     /locus_tag="PK754_01570"
     CDS             13045..13794
                     /locus_tag="PK754_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08225.1"
                     /translation="MFSNTIRGLLLWFIVLNWNAAVNAGDDSCRTKGSLRLRLHSSGF
                     FPYSGRLINRHPSGDIVGTYEKPFISAYVFQSLDLQTRNSDINYFQAGLYRWFHLSDT
                     WSVTPYAIIEIPQNGKWYNSPYAIGGASVLFSPHTSWQIENTTLWADGFKKADRADIV
                     HRLRLTYAPSHRWEWTGFVWANAATFDHQSFYSAAVMGSVSLFSEKHNPDIRLSVMLF
                     QMLRRDHGVGGKNRGIQCSLSIPWSYGSSAR"
     gene            14078..14548
                     /gene="tnpA"
                     /locus_tag="PK754_01575"
     CDS             14078..14548
                     /gene="tnpA"
                     /locus_tag="PK754_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HNB08226.1"
                     /translation="MANTYTQLYIHIVSVVRGRMSLIPDNHKIELYKYMTGIIQNKGH
                     KLLAINGMPDHVHFLIGYDPHEALSALVKEVKRCSTNFINEKGWIKGRFSWQEGYGAF
                     SYAKSQLNTVCRYIENQEQHHKKQTFREEYVALLKRYEINYDERYVFSNVMEEY"
     gene            complement(14693..16330)
                     /locus_tag="PK754_01580"
     CDS             complement(14693..16330)
                     /locus_tag="PK754_01580"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNB08227.1"
                     /translation="MIKRIFKYFGIFFLLIIVAIGLIVGYVYVRHGGFFKYPSGGVLS
                     THQARYDVKSYDITLGVDPEEKFIRGTTTIKIESRADGLDTIQLNLIGLYDVLHVTGP
                     SQPRFTQQEDTLWVIMNQKLDSGSLADIGISYQGTPPTALYPPWFGGFNWSKDESGHH
                     WIGLSCQGEGGKIWFPCKDHPSDEPDSVALHITVPQPYVVAANGILKNITTTSHGMHT
                     YHWVTRYPINNYDINLSIGRYDIVERSYAAASGQTMPVQFYVLPQDRAKADTLIDMAV
                     DMLSTYRLYFGEYPFLSEKFGLVQTDYLGMEHQTINAYGNNFEYREFAGHRWDELMLH
                     EMGHEWWGNKVTVNDWADFWIHEGICSYGEALYQLSQTGEAGYHNHMSRLRKRIRNKK
                     PLVVHPNATTSEAYNDDIYSKGAYLMHSLRFMLGDSVFFKKLYRFANDSAYTYQNRVN
                     TNDFVRLWADVEDGNTTLFIREFIYTTNLPEITIDSTAAETYVIRSRNPKYAMPLEIQ
                     TSRGLERITVHTEGTRIQSGTTPVVDPRRWYLLKSEK"
     gene            complement(16451..16972)
                     /locus_tag="PK754_01585"
     CDS             complement(16451..16972)
                     /locus_tag="PK754_01585"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNB08228.1"
                     /translation="MLNAKLKPYAPYIGILLVLGILLMYSQMRQRKYEAKIEKVFDFA
                     ATEVTEFTVSKDDVKVTIAKGDSSWFFAAPDTGTADDYRVKQFIREVLGATREDAVSE
                     DTIKYNEYGVTESSAVKLELRANGKQLSKVYLGQSKKDIGQEYVRYAGDDRVYPLRGR
                     VISNLSAAASWWR"
     gene            complement(16965..18521)
                     /locus_tag="PK754_01590"
     CDS             complement(16965..18521)
                     /locus_tag="PK754_01590"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HNB08229.1"
                     /translation="MLKITNKSTFINYVLLIAAIVVMANIVSRNLFVRWDATDNKQYS
                     LSESSKEVIGKLKDPMQVKVYFSDNLPGPLANTRRYVQDLLEEYQAFSDGQFVFEFVN
                     PDADDKAKEKAQQLGIGPVPVQAVENDKMEMRNVYMGMVLLYGDKKEPIPVIQSTEGL
                     EYMITATMKKIAATNLKNVGIISPENEEISTQNLNRFLDQLYTVRSVSLGSDISADIQ
                     TIIMNGVSDSVKLDDLYRLDQFLMRGGKLFIGQSNVKDFFQQGFATDIRSNIFTFLEH
                     YGVRIGKEMITDQSCSQIQMQSQQGFFMMRTAVDYPPFPMIRNFNTSHILTQKMTVAR
                     LFFVNEVSSAEAAGTTFTPLMFTSDKTGMLNGPFYQINPTQNPMMKAFPFGTKTVAAL
                     VEGTFTSFFKDSARYASRAGFIPANNNGQIIIVTDNQFFNDRRAGGVQENTDFILNAV
                     DYLSGDKELIAIRSRDVSMRPLAEMTDSERRSWKWINILAPGALVILFGLYMWRRNRQ
                     QRKMLEDRYA"
     gene            complement(18553..19269)
                     /locus_tag="PK754_01595"
     CDS             complement(18553..19269)
                     /locus_tag="PK754_01595"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNB08230.1"
                     /translation="MPNTHMIYRKEMAVFFNSPVAYIVLAVFAVMTSIFFSNTFFLNN
                     QSDLRPLFEVVPWVFMFFVPAITMSLVARERQSGTLEFLVTLPVTDSQIIIGKFLAAF
                     TLVATALGFSLVHFFTLIAVGKHVDVGAVLCGYFGLLLVGALYCAIGIFCSTVTDNQI
                     SAFILTLLIITVFFFFFGKMVTFVPAFLAGLVQYMSVDHHLEGISRGVVDSRNLLYFG
                     SGIAFFLLVSTRLLEMRKWR"
     gene            complement(19262..20209)
                     /locus_tag="PK754_01600"
     CDS             complement(19262..20209)
                     /locus_tag="PK754_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB08231.1"
                     /translation="MIRVENLTKDYGSVRALNSISFEVQDGEILGFLGPNGAGKSTTL
                     KIITSYLAPTSGNVFINDRNILDHSLEIRKQIGYLPELNPLYYEMTVYDFLKFVAAAR
                     QIEGSAFTKRLGEVIELCGLRGVVHKTIAELSKGYKQRVGLAQAIFHDPKILILDEPS
                     TGLDPNQIVEIRELIKNLGKKKTLIMSSHILQEVQATADRMVIINKGQIVANGTTAEL
                     MSGFKGKTVVTMELLNAQEEAVRKLQGAVGGITVTQVTRDSNALMVRLEYPNQSDPRK
                     EIFEYAVKQQWAILEMSRHKVSLEDIFRTLTVEGGQPHA"
     gene            complement(20437..21024)
                     /locus_tag="PK754_01605"
     CDS             complement(20437..21024)
                     /locus_tag="PK754_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HNB08232.1"
                     /translation="MNGEEYLIVHHKNLYKEIEEVIVGIDADASRTKISKEKRKIGNP
                     LLSPIDLNKAFNDAFSKRKWKESRYNYYITLDRELMEKSVLMPAKQQREFLIENGVNE
                     PIYSYNQTDFVKDKIAVEVQFGKYAFVAFDLFVKHMLFYSGGKINLGIEILPTKKMQS
                     QMSSGVAYYEGEVYNVMRQGRNSPPVPLLILGIEP"
     gene            complement(21133..21294)
                     /locus_tag="PK754_01610"
     CDS             complement(21133..21294)
                     /locus_tag="PK754_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08233.1"
                     /translation="MKRIWVLIYIHHGLIQEPEIFNDENSALLRKKEILKKINKDYDE
                     VELFEKQLA"
     gene            complement(21299..22207)
                     /locus_tag="PK754_01615"
     CDS             complement(21299..22207)
                     /locus_tag="PK754_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08234.1"
                     /translation="MPNQLKIGFLKSLEARFGKLKKLPNSLSLFEIGDGLCRIYIRYS
                     KVHSRNQSFYGIRKDDLKQLEGFNSVICFLWDTQTEPLFIPYEEFEEVFSGLEPASDG
                     QIKAQIYQEKDGTELYIANAGRFNVEAFIGWSILNLIVDKNKIIAIPDFSHSQIQTLI
                     GSIGVHHGYDIWIPNIDRNKLDWQLAEKFICKNDLPDRYEKVFDVIKQVDVLWLKRGS
                     SDLSAMFEVEHSTSIYSGLLRFNDLHLVEPNLKPKFSIVSNDLRRALFLRQINRPTFK
                     VSGLSEICNFLEYKDVFGWFNRTIKS"
     gene            complement(22280..22894)
                     /locus_tag="PK754_01620"
                     /pseudo
     CDS             complement(22280..22894)
                     /locus_tag="PK754_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225445.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(23303..24106)
                     /locus_tag="PK754_01625"
     CDS             complement(23303..24106)
                     /locus_tag="PK754_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013408733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNB08235.1"
                     /translation="MIKPGEIQQKANTVGVRDQQIEKDYILSWILFGISKHEPLSKTI
                     VFKGGTVLKKVYFEDYRFSEDLDFTLLNAELPNEQIFAWFKEVFEFIKDEANIPLDII
                     DNNEHEDGGINFYISYIGPLGGQGNNKRVKVDISRSEQLVFEPVMKNVFVDYTDLHEH
                     SLLCYPLEEVLVEKMRSVMQRMQARDLYDIWYLLDKQSMEPDFYIREFETKCRCKNLQ
                     NTDFSQKLSDRLPQYKGRWASSMGEQIKDLPDFDQVEREVLRHIKKLKH"
     gene            complement(24103..24924)
                     /locus_tag="PK754_01630"
     CDS             complement(24103..24924)
                     /locus_tag="PK754_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNB08236.1"
                     /translation="MNNKNISTRSSELLSFFNGQSRNCFDYSLAYKALPDSNASAVRE
                     LLSDMTKRGLLMRLKDGVYYIIPYEQNAKTFMPDWHLISEHLVNDAKHYIGYYSALQI
                     HNLITQPSLKEQIVVSKQIRPSEIKIKEVPFQFIYHNEKHFFGSKKIWIDSFNKVMCS
                     DLEKTLIDCLFKPDYAGGVVEVARAIYTSKDKIKYDTLLDYAKKFDSQAVIKRLGFLL
                     EILDINTQIVQELKQLKTASYVVLDTELPKTGKRISRWSIQQNLEIETIKSAIYT"
     gene            complement(25052..26248)
                     /locus_tag="PK754_01635"
     CDS             complement(25052..26248)
                     /locus_tag="PK754_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HNB08237.1"
                     /translation="MKDAEASRHLRRITLESQGLTKPAPFGKGKAAVLRAMEHLGYIQ
                     IDTLSIVERAHHHTLWTRIPDYKAEYLDQLVAERKVFEYWFHAASYLPVRDFRYALPQ
                     MMRFKRGPSPYYNADPKVMQYVMDTIRTEGPKKARDFESRKKRSGSWWSWKPTKLALE
                     RLFMQGDLMISERDGMQKTYDLRERVLPETMHVTEPTPMEWAEYLVLTYLRAYGFTTI
                     RQITHLKTDERLRKNVQDILQSMLAKGAVQCVDIKGRPSVFTLSEWTEKTFKKNDPSI
                     RLLSPFDNAIIHRDRVKHFFDFDFRIECYTPQKKRLYGYFCLPVLWGDTFIGRVDCKA
                     HRKDQRLELIHLHIENTELPFEIWAEAFIESVRRFAAFNGCSAIVLTKVSPSRYKSVL
                     KKILKK"
     gene            complement(26530..27780)
                     /gene="rho"
                     /locus_tag="PK754_01640"
     CDS             complement(26530..27780)
                     /gene="rho"
                     /locus_tag="PK754_01640"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNB08238.1"
                     /translation="MNLAKLKDLKISELNDIAKDLGMLSFTGMKKQDLIFTILEEQTK
                     RDGFIFSEGVLEILPDGYGFLRSQNYNYLPSPDDIYISPSQIKKFGLRTGDVISGQIR
                     PPKESERFYALLRVEAVNFENPEEAKNKILFDNLTPIYPKERLELETKNANNYSMRIM
                     NLMTPIGKGQRGLIVAPPRTGKTILLKNIANSITENHPEVKMIVLLIDERPEEVTDME
                     RSVKAEVLSSTFDEPPERHVQVSDIVLEKAKRMVECGHDVVILLDSITRLARAHNTVV
                     PHSGKILSGGVDSNALHRPKRFFGAARKVEEGGSLTIIATALIETGSRMDDVIFEEFK
                     GTGNMEIILDRRLSDRRVYPAMDINRSGTRKEELLLTQFEQSRVWILRKLMSDKTPVE
                     AMEYLLDKMRGTRSNKHFLESMNG"
     gene            complement(28167..29117)
                     /gene="serB"
                     /locus_tag="PK754_01645"
     CDS             complement(28167..29117)
                     /gene="serB"
                     /locus_tag="PK754_01645"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HNB08239.1"
                     /translation="MFSRDNKTVIVFVGLQPLPESAWVHVLSSLAEITAVHDRRWLSE
                     FRAIEIYSVCTDKARMHAHIAKTLPDFLQRYAIDYFILDLATRSKKILVTDVDSTLIQ
                     NECIDELARMAGVEKTVSEMTASAMHGAIPFDEALRKRVALLRGLPASALEEVYANAI
                     RLTPGARTLSTTLREQGLHLVMVSGGFTYFTARLSEELGFHHHHANTLEIRDGVLTGE
                     VSGPIIDKETKRHVLIQHCATLRFDTSLAIALGDGANDLPMLITAGDGIAYHAKPIVE
                     QTVMNRIRYNDLTAVLFALGFADYQFSAIKEENSVKLKKT"
BASE COUNT         7856 a         6415 c         6792 g         8492 t
ORIGIN      
        1 cgagcgaacc cgcgattacg gttccaaaag aaagatcgcg ttccgtacga cgcacggatt
       61 cttcggattg tttgtttttc agtgaggcga cactgccttt aaaagaactc acaatcgtgg
      121 gaagcgtttt gatcaatgta ataaatccgc ctgcggcgac agcgccggcg ccgatctggc
      181 gcacatacgc acgatagacg gctgtagcgg tactgccaaa agtttgcgta gcaggatccc
      241 agccgcccgg tccgcctgcg gtgaggatat ccggcagata acccagtttg acaagttgtt
      301 cagctatcgt tgcggcagga accagcgagg ccaaaagcgg tatcaaacct agccatgcga
      361 gcacaccgcc ggctacaagc acaccggcaa tgcgtggtcc gatgatatag cctacgccca
      421 tgtattccgg cgtgatttca ccatttaccg tggcggaagg aaaaaattta ttagtttgtt
      481 gtgtgaccca ggccggtgtt tcggcgatga agtgaaatac tttctggaag aatgcataaa
      541 tgaatgcaaa gccaagacct tgatacgccg ttttggaaaa gtcaccgcct ttttcgccgg
      601 cgaccaaaac ggatgcgcaa gcggttcctt ccggataagg aagtacgccg tgttcctgca
      661 cgatcaacgg tcggcgcaga ggcaccatca tcagcgttcc taaaataccg ccgaacatgg
      721 ctaatacaaa aatcgtccaa taattgaaat aactcggccc catgctttca gcgctgagaa
      781 agagaaatcc gggcaatgta aaaaccacac cggcagcgat ggattcgcct gccgacccgc
      841 cggtctgaat gatgttgttt tcaagaatcg ttgttttaaa aagcttgcgt cccaatgaga
      901 tcgccagcac agcgatagga atggaagctg atacggtgag tcctgctttg agcgcaagat
      961 agaccgtagc cgcgccaaaa atgataccga aaagcgcacc gaggatcagc gctttggccg
     1021 taaactccgg tacattgtgt tccggcgcta tataaggcgt gaagtgatgc gaacccgtcg
     1081 tcgaagagcc cgcttgagcg tcatgtgcca tagattacct tttccgttag tataacgaag
     1141 atgtatagaa agtattttga atggtatatg gaagtgccag cgacaggctg aaaaatccgc
     1201 gaaaaatata ggtcggcatc ccaataaagt caaaccgttt ttagagaaaa tattttgatt
     1261 ttgcgatact ttgattgtat ctttaaaacg gttttatact acgtattctc attatgacag
     1321 gaaacggcat gtctcagccg ctaacaaaac cggttttgct catcgtagat gatgaagtcg
     1381 aaattttaaa ggttttgcaa aaatcacttg aagacgatta cgacgtctta acggcatcgc
     1441 gtgccaaagc cggtctggag cttttggacg aaagggtatc cgtcgttatt tcggatcaac
     1501 gtatgccgga aatgaccggt tcagaattct tacgtcatgt ccgcgaacgc cgccccgaag
     1561 tggttcgtat tatcatgtcc gggtactctg atatgacggc tttgattaat tccgtaaact
     1621 acggtgagat tttcagatat ctcaataagc cttgggatct caacgaatta ctcaatacga
     1681 tagagttggc tgtcaaacgg tatgatgaaa atcgccgtca gatagaatta gcctctgaaa
     1741 atcagcgtct agccgctgaa aacctccgcc ttaccgaaga ccttcacgaa aagacaaccg
     1801 aacttaaccg cctgcgcgga aaataattcc ccgaattcta ttctatcatg cttaaaacca
     1861 tacctcatct gctgatcagc gatgcgcaag ggcgatttag cgacattccg cagattgcca
     1921 tggccgggcg tagtggaaaa aacacggttc ctgttgcagc cgatacattg atccctctgc
     1981 cggaaggttc gcaattatat ttcttacccc aacgtaaggc tatggggtac gatagaaaaa
     2041 ccggcgaact catagtattt gatgatgcgt atgctgtcgc agcatacatc ccgccgtcat
     2101 atacgcatta cctcatggcg gcctacgaaa ccatcacggg agcgccccgg cttccgcttt
     2161 tttcttttac cgcagtgggt tattatcgtg gtcgttacta cgtacctgcc gtgcatgtcg
     2221 atgaagatat caaacagttg ccgacgtctt ttgatgataa aaaagtgcag cgcgctgtag
     2281 atgcgtggat cgttcgtcat cctaataatc gtttattggc gcatcatggg catgcctgtg
     2341 ctatcgaata cggttgtcct aacgctaaaa atctttttct cggacgttgg gaggccccgg
     2401 tcgctgtagc ctcggcgtgc aatgccaatt gtgtcggttg catttcattt caaccgaaag
     2461 aatctgttcc atcaccgcaa aatcgcttag actttgttcc tacggtcgaa gaaatcgtcg
     2521 aatatgccgt accgcacttg gaaaaggccg aacatgccat tttgagtttt ggtcaaggat
     2581 gcgaaggcga accgctgtta cgcggtaaac tgatcgaaga ttctatacgg gcaatacgtc
     2641 gacacaccaa gcgtggtgtt attcatatca acaccaacgg cagcaaaccg gatactttgg
     2701 cgcggttgtt tgatgcgggc ctcgacagtg tccgcgtgag catgaactcg gctcagctgg
     2761 atctttatca tcgctattac aaacctaaca actatacgtt tgacgatgtg gtgcgatcac
     2821 tgaaggtcgc gcgcgatcat aaaaaatttg cctcgatcaa ttattttgtt tttccgggta
     2881 taacggattc ccaagcggaa atacgtgcgc taatggatct tatagccgat acaaagcttc
     2941 atctgattca gtggcgcaat tttaatattg atcctgattg gtatctgaac gatgtcgttg
     3001 gcgattatca atccaaagcg gccggtgttc cttctttgat gcggcgtgtt cgcgaaaaat
     3061 ttccacatct gctttttggg tatatgaata aaccggcgga ggtaattaga gacgctgtag
     3121 atcgctcgac ttaagcagga tgcacgaaga tacaagttta gttcacacct tcatgatcgc
     3181 ggacaaccgt ctgccgaacg tttttacgat atataagtaa tagcttagtt gtaagcgtag
     3241 caaatttcag tttgcaaatt taaatgcgcg atggtttggt attgttctta gcaatgaacg
     3301 catagatttg tcgggagtca cgcattataa aaaacatgaa acataaacca attaaaatcg
     3361 taaagactta cgcaagtcta taccgttcat ctccctatta aatttataag aggtaattta
     3421 gttttatgaa aaagcttctt tcctgtttga ttctttttta tctgtcggtc ccgtccgcgg
     3481 aaggttcaga gcgtacattg ccttattttg tttcaaccca gtggttggcc gatcatatca
     3541 atgattctga tctagtgata cttcaaaccg cttttacacg aaaagagtac gctgtagggc
     3601 atattccggg gtcaagattt ttatggttta actggctcgc tatggatacg ccggatttat
     3661 ccacggaaat gccggatagt gtaacggcta aaaatgtttt ggaaaatctt ggtatctctc
     3721 aaaaatcaaa aattgtcgtc gtgtttacca gaggtagtgt taccaccacg acgcggatgc
     3781 tcgtcgcgct ttcttatttt ggatttgata atcagacagc agttctggat ggtggtcttg
     3841 atgtatggaa ggcggaaggg cgtccgatta ccaaggatat accgaccgta caaaaaacag
     3901 ctctggcgct taaactccat ccggaaatca tcaccaatgc agaatgggtc aagtcgcgtt
     3961 tgtcatccga aaaagttaaa atcatagatg cgcgttctaa aaatttttac gccggcaatg
     4021 gcggcgggat attgcgaacc ggtcacattg aaggcgccag aaatattccc ttttccacat
     4081 tgacggatag taccaataaa attttacccg tcgccagttt gcaaaaaatc attgatgatg
     4141 caggcatcca aaaaggcgac acggttgtca cctactgtca tgtcgggcaa caagccactt
     4201 tggtatatac agttgcacgt atgttagggt atgaagtttt attatacgac ggatgttttg
     4261 aagactggaa tgtacgtgat gaatcgtatc cggtaaaaaa atagaccatg aaaccgatga
     4321 aaccttatat gaatccctat gtggcgggtg tcggacttgg gttggtttta ctcttggcct
     4381 ttttcattgt gggacgaggt ctgggtgcct ccggtgcatt gagttctgtt ttgacttcga
     4441 ccgtaaatac ggtcgcacca tcgcatatcc agtcgaacgc tttttatagt gaatattaca
     4501 ataacggaaa ccctctcaaa gactggttag tatttgagat ggccggcgtt ctgatcggcg
     4561 gcttattatc cggttggttt tcgggaagat taaaatttaa ggtcgaaaaa tcagaaagcg
     4621 tatccacctg cacgcgtttt atttttgctt ttgccggcgg aattctgatg ggtttgggtt
     4681 ccaagcttgc tcgcggatgc acgagcggtc aggctttgac gggaggtgca ttgatgagtg
     4741 taggcagttg gatatttatc gcttccgtgt ttataacggc gtatctggtc gctaaatttt
     4801 ttagaaggca atggaaatga acgccccttt tttgaaattt gacatgatca gttttgaaca
     4861 atcgctgata atagcggttt tcatcggtat tggattcggt ttttttctcg agcaagccgg
     4921 ttttgggagc gccgtcaaac tagcgcagca attttatcta aatgatctct ctgtattcaa
     4981 agttatgttt tctgcgcttg taacagctat gttgggtttg ttttggttga ataaactgga
     5041 tttgctggat ctttcggcta ttcgtgtgct tcccacgtat atatatccgc agctcgccgg
     5101 aggcgtcgtt tttggtgtcg gatttattat gggcggatat tgtccgggta cgtgttgtgt
     5161 ggcttctgtt accggtcgaa tggatggatg gattcatttt atcggaatga tgacaggaat
     5221 ttttattttt gcggaggtct atccctttat cgagagctgg tatttttcaa catcgctggg
     5281 agatatcacc ttgtttcagt tttttcaaat cccgtacggc acggctgttt ttatcgtagt
     5341 tatgatggcg ttattaggct ttgtagccgc tgaacgtatt gaaaaattac atgcaaagga
     5401 ttgaacgcgt gacggttaat cgttttttat ttgcaatctg ttttgtgttc ggtcttttag
     5461 cgctgctttc aggcaattca gatccttatc atgacaagat caatgaaata gcggatcttg
     5521 tcgaaacaga aaacgatcat gttttgcccg attcattagc tgtttggctt atgaatgaaa
     5581 aacccatgcg tatcattcat cttggtgatt cgtcatcgta ctcccgatac cacattcccg
     5641 gtgctgagtt aaagtcgctt cctgatctgt tgcattccac cttgaaaaaa aatgaattga
     5701 taattctata ttccgaaggc ggcattcatg ccgggcaagc atgggtgcta ctcaaagcta
     5761 aaaattttaa aaatgtgtat acgatgcacg gaggattatc agcctggcgt tcggaaatat
     5821 tgtttccaag gttatcgcct gatgcaaaag atgcagagaa aaaacgtttc gaagaaagaa
     5881 gtaagataag tctctacttt ggcggccaac ctacggtgat cctggaacct aagcccgaag
     5941 tgacgatgaa gaagcaagaa ccgccccgaa aaaaaataga tactccgcga tttgaaaaag
     6001 aagaagaaaa aatcagagac ggttgttaaa actattttcc gctgctgagt acccgaaacg
     6061 gcagcctaaa taatgcccag ataaacttga gtacaccctc caccgccaat ccggcaattc
     6121 gaaacggcag caatatcaac catacaaatg ggtacatgat caaggcgacc agggcaagcg
     6181 gccaacacaa gatcaacaaa atacaccaga taaaaaatgt agccatagat tttttatcta
     6241 gtgtatacgt gaaacctcat gccgggtttc aatggagaaa cccgtcgtac atccggtgac
     6301 agtttattga tgattaggga agacgtattt tccgtctcgc atgcgaccgg agaccgactc
     6361 caataaacga cacacctccg gcatgatcga ataaaaatct gaataacgat ttcgttcttt
     6421 atcgtaatcc tccagaagac ggaccatttc ccccatccaa taaaatcccc atttttcctg
     6481 cgttgtgatt tccttttcaa gagaaatgag ttcgggatgg tgccgggtca gatagatgat
     6541 cgatgtggcg cgaaccaatg attcgtacac gagataccgc gggttattat aaaaagcatc
     6601 tttcatcaat gaccacacat gcggatcggc taaaatcagc tccgccggtt tttgccattg
     6661 gctttcgtac gcgtccacag cttgatttac aaacgcgtgg atgtattcgt gtaaaatgga
     6721 gcgtataatc tggagcgaat ccagagacac tggcagaccc caatgatcga atgccgtgca
     6781 gggaaatagc gtaaaggttt gccgcatttc atgaggtgta tttacacgca aataggcgcc
     6841 ctgacgcaat tgcaaaccaa gaattgcgaa atacgcctcc gattcggcca tgccgaaata
     6901 tttctgtaac cacgtttttt gtaaatgcat tgtgcgatgg ttcacgctcg cttcggcacg
     6961 tgcgtaataa tcccgttcat cgtcaaaaaa tcggcgtgca tcgctgtcat aataaaacgc
     7021 tctgatcagt tccattcgcc ggggtgtgag ccaggtgcga ttgtcccaaa tatcgtcaac
     7081 gggcggatcc gctttcgcca cggacgtcca gtccgcgaat gcacgtatat gaatcgcaat
     7141 ggcggggata tcccaataac caaattcgat gtcgtgtttt ttaacgctat ccaggagaag
     7201 ccggatatct tcgatcaaag gatggttctt gtgcttttga aaatgggcat ctatccgctg
     7261 cgtgtaggcc gagtttaacg tgtctattac ttccggatat tccgccaagc gaaaagcaat
     7321 acaaagtaat tcaactcgcg gatctacgcg caacatcaat tcattggcgg gtgtttgaga
     7381 aaacacggtt tcactcaatc cgaataaggt taccatataa acaattccaa aaaatcgatt
     7441 catcaccatc tcctgcatgt gtgaggattt atctcaataa tcattttttc ttaagcgctt
     7501 ccaaaaattg attttcatat tgcgtaacgg ccgtcatggt gttggcatca tttttaaatt
     7561 ttgctttcaa tgcatcaaaa acttctttgc ctctgggatc cgccagtgtt gcgatgagta
     7621 gagcgttatt aagaatatcg ttgatgtcgt tttcctccat cgctttttgg aaacgatcta
     7681 caataatcgg gtaagcctta gccgattggt tcagtgcaac caatgtttgt gcggccgcga
     7741 tacgaaagtc ccataccgga gtgaccagcg tccaacgggg ggcataccga tcactcagtg
     7801 ctgcgaccgc gatatcggct ccgcgcgcat cccccaattc tctcaaacca ttgagcgcac
     7861 tgatcaggct ttggtttttc catgaagggc gacgggtcaa tttactcaaa gcgtcaaacg
     7921 ctttagagtg tttggttttg cccaaggcga tggcggctgc gtttacgaca cgatctttag
     7981 gatcgcggag caaatcaata aagagcgaag cattcgatgg atcccgggta gagcctaata
     8041 agcttatggc tgcggttttg caccacatgc tgtccctgcg aatgacggta agaagtaagc
     8101 ggcgcgtttc attatcaagt accagtttag gattcgaagg cggcgctatc agtgattgaa
     8161 gttgaagcgt agccatgtaa cggagtcgcc aatatgccgt acctccgatc acagtgcgaa
     8221 aagcagcata aatgttttgt ttatcgctaa gcgatgtgcc ggagtctgag gccatcgcgg
     8281 aaagtcgggt catcgcatcc catcgggcta aaatatcacc gtcgccggta aactgatcaa
     8341 tccactctga aaatgttttt tcaaatgttg cctctttgat ccacgtccct tcgtaatcaa
     8401 aaataacacg gcgcggcgcg cgttcgcatg ccaaacgaaa tacattttcc gattgcggtt
     8461 tgatccatat tgtttcgatg cggtcatcta cggctatttc catctttccc tgaaaccagc
     8521 ggttctgtga aaacaccgac gcgattgtat ccggtgattg gatttgacgg actgtaagcg
     8581 tcagggcctt ttccccggga tgatattctt tggttacagt aaacaccggg tgaccggttc
     8641 gatacagcca ttgatcaaaa aaccaatcca tcggttcacc ggtgacggct tcgacagatc
     8701 ggcgcaaatc ctccgtttga acgggacggc cgccgtaagt cagtacatag tgttgcagga
     8761 tttttttcca cgcggtgtcg ccgacatgct tgcgaagcat atgcaaaaca ctggcgccgt
     8821 gaagataagt gtgattaccg gtcaccagag aagcttcatc tgcggtgcgt cgggcaacta
     8881 caggaagccg tataccggat gcccaatcgg agaggtacgt gttatgttga aatgccaatt
     8941 gccacaggag aaattcatcg cgaccatttt tatattcgtc gtacatacaa gacaaatacc
     9001 gcgcaaaggc gcgattaagc caaatatctt cccatgatgc ggcaggaacg taacttccaa
     9061 accattgcat cgccagcgct tcaccttcca gcatgtccca taaaaaaaga aactccgtat
     9121 gcgtcggatg atcatccacc atgttttcgg ttagtgtgga agccgttgta ttttccatac
     9181 cccaaggcaa atcctgaaca aaaacctgag cgtattgtga aaaggggtac gattgcccgg
     9241 taaattcgga ataaaaacgc atcatatcgg gtaaacgttc gaccgtggct acggtagcgt
     9301 cgtactcgtc gggataacta tagttgttga tcacgacacc gtcggtcatc tggcgaaata
     9361 tcgaataacg gccggcgaca aaggatgtac gatgattggc atacggcgta tccattcgcc
     9421 agtgaaatgt tctggtgccg tcgggatgat cttccgtgcg cagcagagcg ccgttagcga
     9481 tgaccgtaaa cggtgtgtcc acacgcatcg aaaatgtggt ggtacgtaaa tccgaaggcg
     9541 tatcatggcc gggaaaccaa taacggttag aagtgtattc ggaaatactc caggcttgtt
     9601 tgcggcggcg gggttctgaa aatgtgggtt caaaaaaacg aatgcctttg ccgttgcttc
     9661 cccagaggtt attcggatca ctatagttga cccatccggt tttgtaaaaa acagtcaagg
     9721 tgaggatttc acctgtgcca agagtccgat ccagttgtat ttgcagagcg ccatcgcgat
     9781 cactgccgtc gtacgtataa cgcaaaggcg tcccatcgcc ggtttcgata cgatgtatcg
     9841 tcagaaaagc gccgtcgagt tcgatacgat ccgcgacggt acgcgtttgc aaaatgatag
     9901 tcgcatatcc ttcgacggat tttgttttcc agtccagacg cagatccaaa tcgatatgct
     9961 gcacatctat tttgtgaatg cgcgcccaac gttccggcgt gataatcgtt gcactatcct
    10021 gcgtgtgcag tggtgtcatc gtcgcaggcc acatcaggca tacatatcca atccataatt
    10081 ttttaattaa tgacctgatc ataccaaact ccggtttatg gtgtataacg gtattccata
    10141 tattcagctg tccgaacggc gacttctttg ccatgacgct gctcaagaat gtgaaatgaa
    10201 agatctatgc ctgcggaaat gccgcctgac gtgtaaagcc gttcatcggt ttgtacaaag
    10261 cgccgatcgt tttgcggttg tccggtgggt acgatttctt ttaagtgatc gtacacctga
    10321 tggtgcgtgc aatagggttt acgatcgagc aaaccgagtg cgccgaagat tcgtgcggcg
    10381 gaacaaatgc tcagagtgta ttcggttgta cgatggacgc gttgtaacca atcgtggtgc
    10441 tcttgatcgc ggagtaacgc tctcgtgcct tgcccgccgg agacgatcag aatatcaatc
    10501 ggaggatgat ttgaaaaatc ataattgggt gtgacgagta aaccgttgac ggcgcgtaca
    10561 ggcttcatat gccgggcgac cgtaaatacg tcatacaagg aaaaatcatg cacttcggat
    10621 gcaatagcaa aaacttcaaa cggcccggcg aagtccaata cttccgcatc atcaaaaatc
    10681 aaaataccta cgtttttttt cataatacct cgtgcgaaaa tcggtgacgt gtatactttt
    10741 ttacaaaata cggctcatga aaaaatttgc atcgccaata aaaagacatt tctggccaat
    10801 aattttttga aataaattaa gctggcttgt gtttttattt tttacagtta tctaatttga
    10861 ttttattggg tttatattgc tgggtaggtt aatactgatt ggccgaaaat gtctgattaa
    10921 tggcgcacgg gttagcataa ccggtgttat ctttaaacca agatcaaagg acgtgttatg
    10981 gcatcacaaa aaattgtttt tctaatattg ccggaagttc atttgttgga tttgggcggg
    11041 cctgagcagg tgtttttgga agctatcggt tacggtgcgc cgtttgaaat cgaatactgt
    11101 acatatacca aagaactgcg aagttcgggc gggctcacgt ttggtcatat caagcatttt
    11161 agcgaggtgg cgatcaaacc ggaggattat atttttatac ccggtccgaa tgtccgatcc
    11221 attcacgatt acaagtttac ttcacaaacc gagttattgg attggttgcg gcgttgctat
    11281 cgtgaaaata tcacgctgtg tagcgtgtgc tccggcgcat ttgcgctggc ttatgcaggt
    11341 ttattggatt acaaaaaatg tacgacgcat ttcaagcgga cggctgaatt gaaacgttat
    11401 tttccaaaac tggatgtaca ggaaaatgtg ctttttgtag aagacggtaa tatcataacc
    11461 agtgcgggga ttgcctccgg tattgatatg gcgttatata ttttagaacg catacaggga
    11521 tcgcattttg cgcacaaagt cgcccgtgaa atggtgatct atacacggcg cacgggttct
    11581 cagccccaaa aaagttcgta ccttgattac cgaaatcaca ttcattccgg tatccataaa
    11641 gtgcaggatt atctttcgga aaatattcat aaaaaatctt ccgtcggtca tttggccgaa
    11701 atcgcgtgca tgagtgaacg taattttaca cgcattttta aaaaagaagc cgggatcacc
    11761 gtcaatgagt acacgacact cgtgcgtaaa gaaaaaataa aattgttact taaaaacccg
    11821 gatttgtcgc gggttcagat tgcccgtgcg gtgggattga atagcgatcg tcagttgagc
    11881 cgaatcatgc attcgatcta atcggataag tatgaatgaa aaatgtttga tataaaaaaa
    11941 ctgcgtcgag tgaaaataaa caatgaaatc acgacccata tttgaactga ggaaatgagc
    12001 atggatatat cggttcgtag agccacgatc gccgatgcga cagcgttggc acttcttgcg
    12061 cgggttacat ttgaggatgc tttcggttac gtgtggaccg ataaaccggt actgaaagaa
    12121 tatctcgaca caactttttc cgttcccaaa atcacatcga gtctcagcaa aacaaacaat
    12181 agcttttgga tcgctctggc cgatggtttg ccggtaggtt acgccaaaat gaaaaaagtg
    12241 tcgccgtaca aattactgga agataacaaa cccgctcaac tgcaaaaaat atatatgctg
    12301 cgcaatttta ttggccagcg tatcggagag cgactacaga gtgctttatt tgacgaggtg
    12361 cgcatgttgg gtattcgtac gctttggctc gccgtctggg atggtaatga caaggccatt
    12421 cggttttacg aacgtcatgg tttccaaaaa acgacacgct atgcgtatga ttttcattca
    12481 atgcattttg agtatgaggt tatgaccaag cggtttgacg tatgaagtgg tgggcggctt
    12541 ccgtgccgtt tggattggcg ttcgtcgcgg cgataggcaa tgccttggtc acctatgctc
    12601 aaaagaaagc gacaccattt gatcacccgt tttattttgg tgctttttca ttgcttctcg
    12661 catcgctcgg attgtttggc atcgcaacat ttttttcttc cggtaaaata ataccctacg
    12721 ccgtggaaaa ttttgtctgg tttgccgttg ccgcagcagg tctgatttta ctcaatattt
    12781 ttttgtacgt gctgtaccgt cattacggag ccgcctatta tacgctgtac gctatactag
    12841 cgatggtcac tacatccatc ggccttgcgg tgctggtttt caaagagtct atgaatgtgt
    12901 acttctggtt atcgttgtta tttgccgcgt tgaccgtggt gtgttttatc aaaggaaaat
    12961 cggggggcta atttgcgcaa gaatcggatt ggaaaacgca acggccggtc ataaattttt
    13021 caaaaaaaca aaggagacat aaacgtgttt tcaaatacga tccgtggttt gttgttatgg
    13081 tttattgttt tgaattggaa tgccgcggta aatgcgggag atgattcatg ccgcaccaaa
    13141 ggttcgcttc gtttgcggtt acattcatcg gggttttttc cgtattccgg gcgattaatc
    13201 aatcgtcatc cgtcgggtga tatcgtcggc acgtatgaaa agccgtttat ttccgcgtat
    13261 gtttttcagt cgttagacct ccaaacgaga aattcggata tcaactattt tcaagccggt
    13321 ctgtatcgct ggtttcatct tagcgataca tggtccgtga ctccgtacgc gatcatcgaa
    13381 attccgcaaa atgggaagtg gtataattcg ccttacgcca tcggcggtgc gtcggtttta
    13441 ttttcgccgc acacttcgtg gcagatagaa aatacgacgc tgtgggccga tggatttaaa
    13501 aaagcggatc gcgcggatat tgttcatcgt ctgcgcctca cgtatgcacc gtcgcaccgg
    13561 tgggaatgga ccggatttgt gtgggctaat gcggcgactt tcgatcacca aagtttttat
    13621 tcggccgcag tgatgggcag tgtttcactt ttttccgaaa agcataatcc ggatatacgt
    13681 ttgtcggtta tgttatttca gatgcttcgg cgtgatcacg gtgtcggcgg aaaaaatcgg
    13741 ggcatccaat gttcgctgag tattccgtgg tcgtacggtt cctcggctcg ttaaaatgaa
    13801 acatcgtatg cctacgggtg tttttagtta ttcacaggtt ttgttgtttt cgctattcag
    13861 taaatattgc taacgcttga atgcaaattt tccctgatct atatttttct taaatcgtta
    13921 cttatttttt ctattagtcg ctttgtaagt tctggcaacg ctctctatgt ctcaggagcc
    13981 attgatcaga ttgtattccc gttagggaat aaaaatggtt aactatgaac tgatagaaag
    14041 cgtgccattc ctgtagggaa tgaattagag gaaaactatg gctaacacct acacacaact
    14101 ttacatccac atcgtttcgg tcgtcagagg tcgaatgagt ttgataccgg ataatcataa
    14161 gattgagctg tataaataca tgaccggcat tattcaaaac aaaggccata aactccttgc
    14221 gatcaatggc atgccggatc atgttcattt tctgatagga tacgatcccc acgaagcttt
    14281 atcggctcta gtcaaagagg tcaaacgatg ttcgaccaat ttcataaacg aaaaaggttg
    14341 gatcaaaggc cgattttctt ggcaggaagg gtatggtgcg ttttcctatg ccaagtcgca
    14401 attaaatacc gtctgtcgtt atattgagaa tcaagaacag catcacaaaa aacaaacatt
    14461 ccgcgaagaa tacgtcgcgt tgcttaagcg gtatgagatt aactacgatg agcgttatgt
    14521 cttttctaat gttatggaag agtattgaaa ccctacgggt ttcatctttc ctttgttgag
    14581 gcattaccaa atttgaaagc ctaacggctt tctgagcgtc atcttttgaa ctaaagcaat
    14641 tcaagaaacg acaaattctt tgttcgtcct tcttagatct ataatccgat gatcactttt
    14701 cacttttaag taaataccac cgtctcggat ccactaccgg tgtagtgccg ctttgaattc
    14761 tagtcccctc ggtgtgaact gtgatacgct ccaatccacg gcttgtttgt atttcgagag
    14821 gcatcgcata cttcgggttt cggcttcgta tgacatacgt ctccgcagct gtcgaatcta
    14881 tcgtaatttc cggaagattg gtagtgtaga taaactcgcg aatgaataat gttgtgttgc
    14941 catcttctac gtctgcccaa agccgaacaa agtcgttagt attgacacgg ttttgatagg
    15001 tgtaagcgct atcgttggca aagcggtata gttttttaaa aaacaccgaa tccccgagca
    15061 taaaacgcag cgaatgcata agataggcgc ctttggagta gatgtcgtcg ttatatgctt
    15121 cggacgtggt cgcattggga tgaacgacga gcggcttttt attgcgaatc cgtttacgca
    15181 accgcgacat gtgattgtga taacctgctt ctccggtttg ggacagttga tacaaggctt
    15241 caccgtagga acaaatcccc tcgtgaatcc aaaagtcagc ccaatcgttg actgttactt
    15301 tattgcccca ccattcatgc cccatttcgt gcaacatgag ttcatcccat cggtgtccgg
    15361 caaattcccg gtattcgaaa ttattgccgt atgcattgat cgtctgatgt tccatcccga
    15421 ggtaatcggt ttgtactaaa ccaaattttt ccgacagaaa cggatattcg ccgaaataca
    15481 accggtaagt gctcagcata tcaacagcca tgtcaatcaa agtatccgct ttcgcacgat
    15541 cctgaggcaa cacataaaac tggacgggca tcgtctgtcc ggacgccgct gcatacgaac
    15601 gttctacgat gtcgtatcgt ccgatgctca gattgatgtc gtaattattg atggggtagc
    15661 gcgtcaccca atgatacgta tgcattccgt gcgatgtggt agtgatgttt ttcaaaatgc
    15721 cgttggcggc gacgacataa ggttggggaa cggtgatatg tagcgctacg ctgtcgggtt
    15781 cgtccgaagg atgatctttg cacggaaacc atattttacc gccttcgcct tgacatgaaa
    15841 gaccgatcca atggtgaccg gattcatctt ttgaccaatt aaaaccacca aaccacggtg
    15901 gatacaatgc ggtgggtggt gtaccttgat atgaaatgcc gatatcggct aaggagcccg
    15961 aatcaagttt ttggttcatg atgacccaga gcgtgtcttc ttgttgtgtg aagcgaggtt
    16021 ggctcggtcc ggtgacatga agcacgtcgt ataacccgat cagattcagt tggatggtat
    16081 ccaatccatc ggcgcgggat tcgattttta tcgtcgtagt tccgcgaata aatttctcct
    16141 caggatcaac acctagtgta atatcgtacg atttgacatc gtaccgtgcc tgatgagtcg
    16201 acaggacacc gccggacggg tatttgaaaa aaccaccgtg ccggacatag acatacccga
    16261 cgatcaatcc gatcgcgacg atgattaaga gaaaaaaaat accgaaatat ttgaatatcc
    16321 gtttgatcat gggaaaaact ccgatggagt ccgctccgtg taacttacga agtatttcca
    16381 ttctgtaaaa ataaaaaaag cggagaagtt ggtttctccg ctttgagata tacgaaaatc
    16441 tgatttcctc ttaccgccac catgaagccg cagcgctgag gttgctgatc acgcgtccgc
    16501 gcagcggata cacccgatca tcgccggcat agcgcacgta ttcctgaccg atatcttttt
    16561 tggattggcc gagatatact ttggaaagtt gttttccgtt ggcacgcagt tcgagtttga
    16621 cggcggaact ttcggtgaca ccgtattcat tatacttgat cgtatcctcg ctcacggcat
    16681 cttcgcgcgt ggcaccgagc acttcgcgga taaattgttt gacccggtaa tcatccgccg
    16741 tacccgtatc cggcgcggca aaaaaccatg acgaatcgcc tttagcgatg gtgactttga
    16801 cgtcatcttt gctgacggta aattctgtca cttccgtggc ggcaaaatcg aatacctttt
    16861 cgattttggc ttcatactta cgctggcgca tctgcgaata catcagtagt atgcccagca
    16921 ccagcagaat gccgatgtac ggcgcgtacg gtttgagttt ggcattaagc ataacgatcc
    16981 tccagcattt tgcgttgttg gcgattgcgg cgccacatat acaaaccaaa aaggataacc
    17041 agcgctccgg gcgcgaggat attgatccat ttccaggagc ggcgttccga atcggtcatc
    17101 tccgcaagcg ggcgcatcga gacgtcgcgt gaacggatgg cgatcaattc tttgtcgccg
    17161 ctcagataat ccaccgcatt gagtataaaa tccgtgtttt cctgcacacc gccggcgcgg
    17221 cgatcgttga aaaactgatt gtcggtgaca atgatgattt ggccgttatt attggcgggg
    17281 ataaatcccg cgcggcttgc ataccgtgca ctgtctttga aaaagctggt aaacgttcct
    17341 tcaacgagtg ccgctacggt tttcgtaccg aaaggaaatg ctttcatcat cggattttgt
    17401 gtcggattga tctgataaaa gggcccgttc agcataccgg ttttgtccga cgtaaacata
    17461 agcggcgtaa acgtcgttcc ggctgcttcg gcggaggaaa cctcattgac aaaaaacaaa
    17521 cgtgcgacgg tcatcttttg cgtcaggata tgcgaggtgt tgaagtttcg gatcatcgga
    17581 aacggcggat agtccacggc cgtgcgcatc ataaaaaagc cctgttgcga ctgcatctgg
    17641 atctggctgc aactttgatc ggtgatcatt tctttgccga tacggacgcc gtagtgctca
    17701 aggaatgtaa atatgttaga ccgaatgtcc gtagcgaaac cttgctggaa aaaatctttg
    17761 acattgcttt ggccgataaa aagtttgccg ccgcgcatca aaaactgatc cagacgatac
    17821 agatcgtcta atttgacgga atccgaaacg ccattcatga tgatggtctg gatatcggcg
    17881 gaaatatcgc tgccgagaga aacgctgcgt acggtgtaca actgatccaa aaagcggttc
    17941 agattttggg tgctgatttc ttcattttcc ggagaaatga taccaacgtt tttcagattg
    18001 gtcgcggcga tttttttcat cgtcgccgtg atcatgtatt ccagaccttc ggtggactgg
    18061 atcacgggga tgggttcttt tttatcgccg tagagcagta ccatgcccat atagacattc
    18121 cgcatttcca tcttatcgtt ttcgacggct tgcacgggta cgggaccgat cccgagttgc
    18181 tgcgcttttt ctttggcttt atcgtcggca tccggattga caaattcgaa aacaaattgg
    18241 ccgtccgaaa acgcctgata ttcttccaga agatcctgca catagcggcg cgtattggct
    18301 aaggggccgg gcagattatc cgaaaaatag actttgacct gcatcggatc tttgagtttg
    18361 ccgatgactt ctttgctgga ttcggagagg gaatactgtt tgttatccgt cgcatcccat
    18421 cgcacgaaaa gattgcggga cacgatattg gccattacga cgatcgctgc gatgagcagg
    18481 acatagttga tgaacgtgct tttattggtg attttgagca taagttttat ccgttaatac
    18541 attcgtaaat aatcaacgcc acttgcgcat ttccaggagc cgggtgctga ccaaaaggaa
    18601 aaacgcgata cccgatccga agtagagcag attgcgggag tccaccacgc cgcgggagat
    18661 accttcgagg tgatgatcca cgctcatgta ctgcacaagt cctgcgagaa atgccgggac
    18721 gaacgtgacc attttgccga aaaagaaaaa gaaaaccgtg atgatcagca gcgtgagtat
    18781 gaatgccgaa atctggttat cggtcacggt actgcaaaaa atgccgatcg cacagtagag
    18841 cgcgccgaca agcaggagcc cgaagtatcc gcacaatacg gcgcccacat ccacgtgttt
    18901 gccgacggcg atcagcgtaa aaaaatgcac gaggctaaat ccgagcgcgg tcgctacgag
    18961 cgtaaatgca gccaaaaact ttccgatgat gatctggctg tcggtcaccg gcagagtgac
    19021 aagaaactcc agcgtacccg actggcgttc gcgggccaca aggctcatcg tgatcgcggg
    19081 tacaaaaaac ataaacaccc acggcacgac ttcaaacagc gggcgcagat cggattggtt
    19141 attgagaaaa aacgtattgc taaaaaatat cgaggtcatc accgcaaata cggcgagcac
    19201 gatataggcg acggggctgt taaaaaacac cgccatctct ttgcggtaga tcatgtgtgt
    19261 attaggcatg gggttggcct ccttcgacgg tgagtgtccg gaaaatatct tccaggctga
    19321 ctttgtggcg gctcatctcc aaaatagccc actgctgttt gacggcgtat tcgaagattt
    19381 cctttcgcgg atcggactga ttgggatatt cgagacggac catcagtgcg ttgctgtccc
    19441 gtgttacttg ggtgacggtg ataccgccca cggcgccctg cagtttgcga acggcttctt
    19501 cctgcgcgtt gagcagttcc atcgtgacga cggtcttgcc tttgaagccg gacatcagtt
    19561 cggcggtggt gccgttggcg acgatctggc ctttattgat gataaccatg cggtcggcgg
    19621 tggcttgcac ttcctgcaag atatgactgg acatgatcag ggtttttttc ttgccgaggt
    19681 ttttgatcag ttcgcggatt tcgacgatct gattggggtc gagcccggtg gacggttcgt
    19741 ctaagatcag aatcttggga tcgtgaaaga tcgcctgtgc cagtccgacg cgctgtttgt
    19801 aacctttgga gagttcggcg atggttttgt gaacgacacc gcgcaggccg cagagttcga
    19861 tcacttcgcc gaggcgtttg gtaaaagcgc tgccttcgat ctgacgggcg gccgcgacga
    19921 atttcagaaa atcgtacacg gtcatttcgt aatacagggg attgagttcc ggcaggtacc
    19981 cgatctgctt gcgtatctca aggctgtgat cgaggatgtt gcggtcgttg ataaaaacat
    20041 tacctgaagt gggcgcgaga tacgacgtga tgatcttgag cgtcgtggat ttgccggcgc
    20101 cattgggccc cagaaatccg aggatctcgc cgtcctgcac ttcaaagctg atgctattga
    20161 gcgcacgcac gctgccgtaa tctttggtga gattctcgac acgtatcata gaaactccgt
    20221 ttgggttaat gaatcatata ctacactaaa aaacataaga ctaagaaaca taagctatac
    20281 taagagtatt tctaaaacgc gggaaagata gataaccgca ggggatttgt caagacgggg
    20341 gtgcgactgt gggtgttttg gggtgattcg atgttcggga ttgcattccg cttcttaaaa
    20401 tttttggaca gaaatttggg cttcctttct tgtgttttat ggctcaatgc ctaaaatcaa
    20461 taatggaacc ggagggctat ttcgtccttg tctcattacg ttataaacct ctccttcata
    20521 gtaagctaca ccactactca tctgcgattg cattttcttt gttggtaaaa tttcaattcc
    20581 aagattgatt ttaccgccag aatagaagag catatgtttt acgaacaaat caaacgcaac
    20641 aaaagcatac ttcccgaatt gaacttctac ggctattttg tccttaacaa agtctgtttg
    20701 attataactg taaattggtt cgttcactcc attctcaata agaaattctc tttgctgctt
    20761 tgcaggcatt aaaacgcttt tttccattaa ctcgcggtca agtgttatat aataattgta
    20821 tctgctttct ttccatttgc gtttggaaaa agcatcatta aatgctttat tcaaatcaat
    20881 aggagatagt aacgggtttc ctattttccg tttctccttg ctaatcttgg tcctagaggc
    20941 gtcggcatca atgccgacaa taacttcttc tatttcttta taaagatttt tgtgatgtac
    21001 aattaaatat tcttcgccat tcaagtggga atacttctgt gcgattttat ttttatgtgc
    21061 ccaactgcat tgcgactatt aaacatcgtt gcgttgcact cttatgctgt aaatcactac
    21121 tgagcaagat tatcaagcaa gttgcttttc aaaaagctct acttcatcgt agtccttatt
    21181 gatttttttt agaatttctt tctttctcaa taatgcagaa ttttcatcat tgaaaatttc
    21241 aggctcctga attagtccat gatgaatata aattaaaacc caaattcttt tcattgtatt
    21301 aagatttaat tgttctatta aaccatccga atacgtcttt gtattcaaga aaattacaaa
    21361 tttctgaaag tccgctcact ttaaaagttg gtctattaat ttggcgtaag aacaaagccc
    21421 gtcgtaaatc atttgaaacg atactgaact ttggctttaa atttggctca actaagtgca
    21481 aatcattaaa cctcaaaaga cctgaataaa ttgaagttga atgttcaact tcaaacatag
    21541 cagacaaatc acttgaacct ctcttgagcc atagaacatc aacttgctta attacgtcaa
    21601 atactttctc atatctatct ggtaaatcat ttttgcagat aaatttttcc gcaagttgcc
    21661 aatccagttt gtttctgtca atattaggta tccatatatc atacccgtgg tgaacaccaa
    21721 ttgagccaat aagagtttga atttgcgaat ggctaaaatc aggaatagca atgattttat
    21781 tcttgtcaac aattaaattt aaaatactcc aaccgataaa agcttctaca ttaaatcttc
    21841 cggcgttagc aatatataat tcagtgccat ctttttcttg ataaatctgt gcttttattt
    21901 gtccatcaga agctggttct aatccagaaa agacttcttc aaattcttca taaggaataa
    21961 acaatggttc ggtttgtgta tcccaaagaa aacaaataac agaattaaat ccttccaact
    22021 gctttaagtc atcttttctt atcccataaa aagattgatt tctgctatgg acttttgaat
    22081 atctgatata aattctacat aagccgtctc caatttcaaa aagagataaa ctgttcggaa
    22141 gttttttcaa cttgccaaaa cgagcttcta aacttttaag aaaaccaatt tttaattgat
    22201 taggcatttt attattctat gctgtgacgt taagttgaga agcatgggag attgaagtag
    22261 aaaattcagt acgaaccgtt gattttacat taggaatgtt ccacatggtt ttatcccagt
    22321 cttgttcaat aatctcccaa atgtcgctag gatttttacc caaagggtta ccggatagtt
    22381 gccccttttt tggacctttg aagtgcagct ttcctggata tttagatgga actctaacct
    22441 tatcaagatt aaaaatgtaa tcgtctgttt tgctaaacca gaggattgtt tcgtatcgac
    22501 cactaaaacg attgctggcg tgaagtccat gcccgaaatg ccacactata cggttgcgaa
    22561 gttttaagcc gtgtttctta aatatttgat aataaaatat atcaagcggg aatatttctc
    22621 ctttgtcgac ataatttcct acctgccaac atagatttcc attttttgat aatgttctta
    22681 caagttcaga aataatttct tcctgcgtat caagataatt ttctatactt gttttagtct
    22741 catatgattt gcctatgttg tacggtggtg aagtcaaaat gaggtcaaat ttattatcct
    22801 caattttctt gagtatagtt aaactgtctc cgtttttaat agtgtagtca atgtcgggac
    22861 tcaaatagtc aaatagaggt tcttttattt tcatcttctt tatcataagt tgttttgcaa
    22921 ttttaatctt aagtggttaa gtgcgtaaaa atcattataa aaatattagt gttcttgttg
    22981 taatatgtag tttccaactt tgtttagtag cgttgtaata aataaaaaca actctttcct
    23041 ccatataaag gaatacctaa gttttacgtt ttttcctttc ttgcatgttg caaaaatata
    23101 atctcaatcc catcaaaaca acacaaatca cagtctacaa gttttagtgt gggtgtacta
    23161 taaaatgata tttctcacct cataatacaa cacgatagaa ccggagctga atattttggt
    23221 gttggtgagt ttgagtattc tcttttcact gatttgttta aacagaggta aactttttat
    23281 gcgcttccgc agcggttcgt ttttagtgtt tcaatttttt gatgtgcctt agcacttctc
    23341 tttctacttg gtcaaagtcg ggcaggtctt ttatttgttc gcccatagag cttgcccacc
    23401 ggcctttgta ttgaggcaat cgatcagata gcttttgcga aaaatcggtg ttttgcagat
    23461 ttttgcatct gcactttgtc tcaaattcgc ggatataaaa atccggctcc atgctttgtt
    23521 tatccagtag ataccagata tcgtataagt cacgggcttg cattcgttgc atcacggaac
    23581 gcattttttc aactaacact tcctccaagg ggtagcaaag caatgagtgt tcatgcaaat
    23641 cagtgtagtc aacaaataca tttttcatca caggttcaaa caccaattgc tcactgcgtg
    23701 aaatatcaac ttttacccgt ttattatttc cttgtccgcc aagcggacca atatagctga
    23761 tgtaaaaatt aatgccgcca tcttcatgct cgttgttgtc aatgatatca agcggaatat
    23821 tggcttcatc ttttatgaat tcaaatactt ctttaaacca tgcgaaaatt tgttcgttag
    23881 gtaattcagc attcagcaat gtaaaatcta agtcctcaga aaaacgataa tcttcaaaat
    23941 agactttctt taataccgtt ccacctttga aaacgattgt ctttgaaagt ggctcatgtt
    24001 tagaaatgcc aaacagaatc catgaaagga tgtaatcctt ttcaatctgc tggtctcgca
    24061 caccgactgt atttgctttt tgctgtattt ctccgggttt gatcatgtgt aaatggctga
    24121 tttaatggtt tcaatttcca agttttgttg aatactccaa cgacttattc gctttcctgt
    24181 tttaggcaat tcggtatcaa gcaccacata cgatgcggtt tttagttgtt ttagttcctg
    24241 aacaatttgg gtattgatgt ctaatatttc taaaagaaaa cccaaacgtt tgattaccgc
    24301 ttgcgaatca aattttttgg cgtagtcaag aagtgtatcg tattttattt tgtctttaga
    24361 agtgtagatt gcccttgcca cttcaacaac cccgcctgca taatcgggtt tgaataaaca
    24421 gtcaataaga gttttttcca agtcggaaca catgacttta ttaaagctgt caatccagat
    24481 tttctttgaa ccgaaaaaat gtttctcgtt gtgatagata aattgaaaag gaacttcttt
    24541 gatttttatt tctgatggtc ggatttgttt tgaaacaacg atttgttctt ttaatgatgg
    24601 ttgtgtaatg aggttatgta tttgcaaggc tgaataatat ccgatataat gtttggcatc
    24661 attcaccaaa tgctcagata tcaaatgcca gtcgggcata aaggtttttg cattttgttc
    24721 gtatggaatg atgtaataaa caccatcttt caaacgcatc aacaaacctc tctttgtcat
    24781 gtcgctgagt agttctctga ctgcacttgc attagagtcg ggcagggctt tgtaagccaa
    24841 agaatagtca aagcaattcc tgctctgtcc attaaaaaaa gacaaaagtt cgcttgatcg
    24901 tgttgatatg tttttattat tcatatggcg tatgtcaggt ttaagctgac atttatacta
    24961 taaaaaataa atcaatagcg ctgtaatgtc aatttaattg tgggttgata atcagctaga
    25021 ctcaagccga cttatttgat ataatcatga cctatttttt caaaatcttc tttaaaacac
    25081 ttttgtaccg ggacggactt actttggtaa gcacgatcgc ggagcagccg ttgaacgcgg
    25141 caaaacgtcg cacggattca atgaaagctt ccgcccatat ttcaaaaggc agttctgtgt
    25201 tttcgatatg caggtggatc aactccaacc gctgatcttt tcggtgcgct ttgcagtcca
    25261 cgcggccgat aaaggtatcg ccccataaga caggcaaaca aaaatagccg tatagtcttt
    25321 ttttctgcgg cgtgtagcat tcgatccgaa aatcaaaatc aaaaaaatgt ttgacgcggt
    25381 cgcgatggat gatggcatta tcaaaaggcg ataataaccg gatcgagggg tcgttttttt
    25441 tgaatgtttt ttcggtccat tccgaaagtg taaataccga cgggcgacct ttgatatcta
    25501 cgcactgcac agcgcccttc gcaagcatgg attgtaaaat gtcctgcaca ttttttcgca
    25561 aacgttcatc cgttttaaga tgggtgattt gtctgatcgt agtaaatcca tatgcccgca
    25621 gatatgtcag aactaaatat tcggcccatt ccatcggtgt cggttcggtc acatgcatgg
    25681 tttcgggtaa tacgcgctcg cgcagatcgt acgttttttg catgccgtcc cgttcgctga
    25741 tcatcagatc gccctgcata aatagccttt ccagagctag tttggtaggt ttccagctcc
    25801 accaacttcc ggatcttttt tttcggcttt caaaatccct ggcttttttg ggaccttcgg
    25861 tgcgaatcgt atccatcaca tactgcatca ctttgggatc ggcattgtaa tacggagacg
    25921 gaccgcgctt aaaacgcatc atctgcggca acgcgtaacg aaaatctcta acgggaagat
    25981 aggacgccgc atgaaaccaa tattcaaaca ctttgcgctc ggctaccaat tgatctaagt
    26041 attctgcttt ataatccgga atccgcgtcc atagcgtgtg atgatgcgca cgttcgacga
    26101 tggacagagt gtcaatctgg atgtacccga ggtgttccat agcgcggaga acagccgctt
    26161 taccttttcc aaacggcgcc ggtttcgtca gcccctgact ttccaatgtg atacggcgga
    26221 gatggcgcga tgcctcggcg tccttcatga tcggctttct tgatttatta taagtagttg
    26281 gcacgcaatg ttgccgaaaa aacagagcaa aaaaatatga cgttcaaaac gtacgaaaag
    26341 aaaaccccga cgaatgctac ggatttttaa tgtaatttac aggctttttt ggtcggctat
    26401 cgtaggacgt acggcgagac gatgttatgt cgtttcatac aaccttataa aaagggcgtt
    26461 aatggatatc aaaaataaaa aagggatgaa tccgagcgac tcatccctta cgttaaaaat
    26521 gaaacgcgat taaccgttca tggactccag gaaatgctta ttggagcgtg tgccgcgcat
    26581 tttgtcgagc aaatattcca tcgcttcgac cggcgtctta tcactcatca gtttacgcag
    26641 aatccatacg cggctttgtt caaactgcgt aaggagaagt tcttctttac gggtaccgga
    26701 gcgattgatg tccatcgcag gatagacacg gcgatcggac aaacggcgat caaggatgat
    26761 ttccatatta cccgtacctt tgaattcttc aaagataaca tcatccatac gcgagccggt
    26821 ttcgatcaaa gcggtcgcga tgatcgtcaa actgccgcct tcttcgactt tacgagccgc
    26881 gccgaaaaag cgtttggggc gatgaagggc gttagaatcc acaccgccgg aaagaatttt
    26941 accggaatgc ggtacgaccg tattatgtgc acgggcaagg cgtgtgatcg aatccaaaag
    27001 aatcacgaca tcgtgtccgc attcgaccat acgtttggct ttttcgagaa cgatatccga
    27061 cacttgtacg tgacgctcgg gcggttcgtc gaaggtggaa cttaatactt ccgcttttac
    27121 agagcgttcc atatcggtca cttcttcggg gcgctcgtcg atcagcagta cgatcatttt
    27181 gacttcggga tgattttccg taatgctatt ggcgatattt ttcaaaagaa tcgttttacc
    27241 ggtacgaggg ggtgcgacga tcagaccgcg ctggccttta ccgatgggcg tcatcagatt
    27301 catgatgcgc atcgagtagt tattggcgtt cttcgtttcc agttcaagac gttctttagg
    27361 atagatcggc gtgaggttat caaacagaat tttatttttg gcttcttcgg gattttcgaa
    27421 gttgaccgct tcgacacgca ggagcgcgta aaaacgttcg ctttccttcg gagggcgaat
    27481 ctgtccgctg atcacatcgc cggtgcgtaa gccgaatttt ttgatttgtg acggggagat
    27541 gtagatatca tccgggctcg gcaggtagtt atagttttgc gaacggagga aaccatagcc
    27601 gtcgggtagt atttccaaaa cgccttcgct gaaaataaag ccatcgcgtt tggtttgttc
    27661 ttcgaggatc gtaaaaatca gatcctgttt tttcatcccg gtaaagctca gcatgccgag
    27721 atctttggcg atatcgttaa gttccgagat tttgaggtct ttgagtttgg ctagattcag
    27781 cgatgttttt tcccggggtg cgggagcttc ttcttcgaca tcggccggta aaccggcttt
    27841 cgaatcggct tttttatctc tgggtgtacg cgcaccgtcg tgtttgcgtg tggttttagg
    27901 ttcagcggtt tccgctacgg gttctgccgc ttcagttttg atttcttttt cttcgctcat
    27961 gttggggtaa gattaagtga aaaacggtta taaagagaaa attaacaatt aagaatgatt
    28021 cagttttata gaggcaacga tattcaacga aaatttgtcc gatttagcgc cgaacagaaa
    28081 attaagtgtt ggaatttaag gatgtgaacc tttgcagatc gaagtctctg tagcgtaccc
    28141 caaagtatat gaatcaccca atattgtcaa gtttttttaa gtttgacgga attttcttcc
    28201 ttaatcgctg aaaactggta atccgcaaac cccaaagcaa acaatacggc cgtaagatcg
    28261 ttgtaacgaa tgcgattcat cacggtttgt tcgacgatgg gtttcgcatg ataagctatc
    28321 ccgtcaccgg ccgtgatcaa cattggtaag tcgttggcgc catcacccaa agctatagcc
    28381 aggctcgtat caaaccgaag tgttgcacag tgttggatga gaacatgccg tttagtttct
    28441 ttgtcaataa taggtccgct gacttcaccg gtcaaaacgc cgtcacgtat ctctaaggta
    28501 ttggcatgat ggtgatgaaa tccgagttcc tccgaaagac gagcggtgaa atatgtaaat
    28561 ccgccggaca ccatcacgag gtgtaaacct tgctcgcgca gtgtcgtact gagcgtgcgt
    28621 gcaccgggcg tgagccgaat cgcattcgca taaacttctt ccaatgcgga ggcaggcaat
    28681 ccgcgaagta atgcgacacg tttgcgcaac gcttcatcaa acgggatagc gccatgcata
    28741 gccgatgcgg tcatctccga cacggtcttt tctacgccgg ccatgcgtgc taattcgtca
    28801 atgcattcgt tttgtatcaa tgtcgaatca acgtcggtca ccaatatttt tttggacctg
    28861 gtcgctaaat ccaaaatgaa ataatcgatg gcataacgtt gaagaaagtc cggtaatgtc
    28921 ttcgcgatat gggcatgcat gcgggcttta tcggtacata ccgaataaat ttcgatggcc
    28981 cgaaattccg ataaccatcg gcgatcatgc acggctgtaa tctcagccag cgaactgagt
    29041 acgtgcaccc atgccgactc cggcaagggt tgcaagccga caaatacgat gactgtttta
    29101 ttatcccgtg aaaacatggc tggtatatat attcccgaag aacctatcca tttaatgtaa
    29161 aacggttttt ggaatattca tatatgtatt tttaaaaaac ctttgacgtg aaaagttttt
    29221 tcggagaaac ctacttgcat cggacagtcg agttagacat cagggatgtt ccaaaatgtc
    29281 cggcagagaa tttcaaaatt cccgattttt cttttcatta ccgatgtcag ttactatatt
    29341 tcgctagcac gtcccaagcg gttttcaagg agatgtttta tggagcagga taataaacca
    29401 acccccaaag aggatttggc cggcgaatat gccgatttgg tcgaaacgcc tcttttttca
    29461 cgattcaaag aatggtggcg cgccaaaaag gttagcctcg atttattggg tgtcgagaaa
    29521 atcatcggtg tggacaacag gcaatataaa gccgc
//
LOCUS       DAOQNQ010000012        27860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_555_length_27860_cov_6.712785, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000012 DAOQNQ010000000
VERSION     DAOQNQ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 27860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27860
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_555_length_27860_cov_6.712785"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PK754_01650"
     CDS             complement(<1..925)
                     /locus_tag="PK754_01650"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNB08240.1"
                     /translation="MEIVIPDTNITAIMPQVILTIGACVTLLMGVFTPHLRKWAGYVA
                     FATCVIAFLDVMTMWDTKMVAFSGMITLNYYTHAFSIVILASAALVCLVTYDDVSEHK
                     VGEFFSVLLMSTTGMLIMVASMNLMVIFLGLEIMSIGLYILISINRSKIRSLEAGMKY
                     FLLGAFASGFFLYGIALTYGATGSFDLARIANYLSMNNLLGDPLALAGTSLLIIGFGF
                     KVALVPFHMWSPDVYQGSPAPVTAFIASGPKVAAFAAFARVFIDTFNTLAIEWSEVLF
                     WICVISMIGGNLMALVQRDIKRMLAFSSISHA"
     gene            complement(951..4301)
                     /locus_tag="PK754_01655"
     CDS             complement(951..4301)
                     /locus_tag="PK754_01655"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB08241.1"
                     /translation="MQSSLTTLCPNCGKELTADTQPAACPHCRYLFSVDLKSFIVPED
                     HGLCVKEIRTGIEKERFEEVGLLIHHLIHRNFLDDKALRMIGMELHATHKDALALACC
                     ERAVQINPQDAWNHHALSETCQQIGMLEAAFKHAQEAVRLDPMESRHHARIAEVYDKQ
                     NQSTLSARSYLMAMALSGAYIKNFDQLIDDAIDLVMVESTYEELRKQHAEVRIIHFAM
                     GNLYLGKMNYEKAETAFQQVISSQPSNVSALNRLAEVYQKQNKPEQALEMYKRSLASE
                     ESNLDTLRNLGKLYKEKSNWAEALKYFERILKLNNKDSNAAYETKEMYRKLNNPQKAI
                     EVMKEYQRTDVSYYNWAMHHIFEILYYDLKDDKQLIELVEKTYPNKITDSFVYDYYGY
                     SLMNTGRLDEAVKAFERFVELEPKNARVMRELGRVFCDLQRYQEAIEVLKKSFAIENK
                     NSYGYYYLALAYHKLGKDDLARKTLNEGKQFDPTYKWFDELLTEMDPNYTPTAIPENV
                     TETPVTTGTGVLGETVAPALPNFLRDLTQLAKEGKIENIFGRTHELMELIEVLCRRNK
                     ANPLIVGAPGVGKTVLVNGLAHLLAQKKVPDILQDYRLLELEVFAVVAGTRYVGTLEQ
                     KFMQLAQYCRQNKVIIFIDELHTLMGAGSYSSHETGGLDEMFKPLMTQPEFKLIGATT
                     DEEYQKNIAKSGAFDRRFTRITVFEPMRDETMNILGSLRGKIEPFYHVQVSDRMLRTT
                     YDMAEKYIKNRYFPDKACDLLERASIKASLSRSGAEGEIKEVAEEHIVQAVSQVTRIP
                     ESNIQIDAMMGMLSLEDRLRMRVIGQEEAIQKVAEVVRMTKSGLDVNPERPDGVFLFV
                     GPTGVGKTELAKALAEALEGSDKRLVRIDMSEYNDQYALSKLIGTAPGFVGYEDEGRL
                     TKAVREDPSAVILLDEIEKAHPKIYEAFLQVFDDGRMTDGKGNVISFSHTTMIMTSNL
                     GAEHIYGKEKLGFSGGTEDVHKQISQGITESMRKHFSPEFLNRIDEIIVFRPLDDEAL
                     IQIAQQKLDGIVKRFADRDIRIHIEQDVAALCVRDIDSKREGARGINRQLEDMISRPL
                     SRVMIQNPNCKAYNIKRSENEILVLRGA"
     gene            complement(4452..6038)
                     /locus_tag="PK754_01660"
     CDS             complement(4452..6038)
                     /locus_tag="PK754_01660"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNB08242.1"
                     /translation="MENYNSLALSLLIFMPLVGVVVISFIDRRNENLIKATTFAFSAL
                     VFVLSIPIYMNYDFSNPEMQFTYKAKWIVDWGAYYTLGVDGLSMLLIMLTTFMMPLAV
                     IASWKSIHFHVKGFMISLLLLDAGMVGVFCARDMLLFYVFWEAMLVPMYLIIGIWGHD
                     HRIKAAVTFFIYTMAGSLFMLVAIIYMYFQTGAVVDNGTVLSMHSFAVDDFAKLQLPY
                     ETQYWLFLAFALAFAVKVPMFPFHTWLPLAHVEAPTAGSVDLAAILLKMGPYGFMRFC
                     IPFFPEVANDFTPVIMVVAIIGIIYGALVAMVQTDAKKLVAYSSVSHLGFVMLGIFAF
                     TIQGWQGAMMIMIGHGISTSALFLMIGMLYDRRHTREIAKFGGLWKVMPAFSVIFMIV
                     CFSSLGLPGTNGFIGEFLVLIGTFISSIGHARLYATLAMLGVILGAVYLLWMYQRVCF
                     GEIVHEENKKLPDLSVREYALVTPFLILIFLLGFYPKILTEKTEATMHKYLNNQKTAV
                     EQAKTKKNSGPMMTNQPIPY"
     gene            complement(6083..8095)
                     /gene="nuoL"
                     /locus_tag="PK754_01665"
     CDS             complement(6083..8095)
                     /gene="nuoL"
                     /locus_tag="PK754_01665"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNB08243.1"
                     /translation="MEMIILMIPLIPLAGAVFNVFFGRMIEGQGSHHTEGHEEGHAHH
                     HAKPWAGYLASGLVFISFLLSLYVFIQVLGGQKYNIDYLDWIIVDTLRITFGFLIDPL
                     TGVMLLIVTGIGFLIHVYSLGYMEHDPDRPRFFTYLNLFIFSMLMLVMGNNLAVMFFG
                     WEAVGLCSYLLIGYYYKKKSAGDAGKKAFIVNRIGDFGFGLGIFLVFATFGTIHFETL
                     FDAIHKVDPYALGTTMTAIGMLLFVGAMGKSAQIPLYVWLPDAMEGPTPVSALIHAAT
                     MVTAGVFMVARCSPIFSFDIGPAGYIMGMSAGDLVTYVGAATALFAATIGFTQTDIKK
                     VLAYSTVSQLGYMFIGVGVGAYAAGVFHLLTHAFFKALMFLGSGSVIHVMEHAYHKAG
                     IHEDPQDIRKMGGLFSKAKITAITFLIGWVAICGLPPFSGFFSKDEILASAYHKGYNG
                     VFWFGVLGAMMTAFYMSRLVFLTFFGESRTPEAAKEHLHESTSTMTVPLMILAVFAAI
                     AGLIGIPPVLGGHNWLAHFLEPSVGTHAAPVEHHGFNDMTLMVISVLAGVIGIGIAWV
                     IYMAKKVSAEKIAADYQPIYKTLLNKYYIDEFYDKFIVEPTKWVSEKVFLRLVDNKLI
                     DGLIYLIAVTLDLFGRILRLFQTGYVQTYAFFISLGVALIIYYIMR"
     gene            complement(8141..8443)
                     /gene="nuoK"
                     /locus_tag="PK754_01670"
     CDS             complement(8141..8443)
                     /gene="nuoK"
                     /locus_tag="PK754_01670"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNB08244.1"
                     /translation="MVPLSHYLIVTAILFSLGVVGVLTRRNAIIVFMCIELMLNSVNL
                     TMVAFARYLDSMDGQIAVFLVMTVAAAEVAVGLALIVAIYRKKDTINVDEMHMMQG"
     gene            complement(8458..8991)
                     /locus_tag="PK754_01675"
     CDS             complement(8458..8991)
                     /locus_tag="PK754_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNB08245.1"
                     /translation="MENMLQYVMVNPVFYIFAAIAVLCAIMMLTMENPVYSAIFMVGV
                     FFCIAVLYITLEAEFIAAIQVLVYTGAIMVLFLFVIMLLNLGRDAHLTEYAGPMKYIS
                     IMLAFAILVQIGLATKVTYNLGDRGIYPIEKIREAGNVEVIGSVLMSQYLYPFEIASI
                     LLLLGMVGAIIISKRKQ"
     gene            complement(9057..9668)
                     /locus_tag="PK754_01680"
     CDS             complement(9057..9668)
                     /locus_tag="PK754_01680"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HNB08246.1"
                     /translation="MAVNVKNVSEPRGKSWLDNTYVIPILKGLGITFKHLFKKRDVLS
                     YPEDKKVLPPNYRGLHMLTRDENGREKCVACEMCSTACPADCIRIVANDSGDAWKDGH
                     ENREKHPKVFEIDELRCIYCGMCVEACPEDAIDMTDMHNLADYTRADFIYNKERLLGV
                     YDEYIKRHPDRAQRRDGLHKQMKNSSYFTVKDVKHSDEHGSVH"
     gene            complement(9706..10728)
                     /gene="nuoH"
                     /locus_tag="PK754_01685"
     CDS             complement(9706..10728)
                     /gene="nuoH"
                     /locus_tag="PK754_01685"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNB08247.1"
                     /translation="MDAFLPFVPYLVIGLMLAIYLAIVPIMLIVERRVSAWVQYRVGP
                     NRVGPLGLFQPIADALKMMSKEDIIPLHVNKWLYVAAPALMVFPAFFVVGVIPWGFIT
                     VGGREIWIQIGQTNIGVLYVLAVSSLSVYAITFGAWASNNKYSLFGGMRSAAQMISYE
                     LPMGMAIMSMVLVVGSLKIEDIVNFQIQNHMWGIVFQPLAALLFIATAFAETNRTPFD
                     LTEAEAELVAGYHTEYSSMKFGLFYLGEYCNMLTNSIMITCLFLGGWHIPFVNDMGFS
                     PLTRDLINLGGFAFKVLFLMFFYVWVRWTLPRFRYDQLMNLGWKKMIPLALANILITA
                     IWLAFV"
     gene            complement(10757..12370)
                     /locus_tag="PK754_01690"
     CDS             complement(10757..12370)
                     /locus_tag="PK754_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNB08248.1"
                     /translation="MALVKIDGQELEMQRGETILNAAKRIGIEIPYYCYHPGMKIVAS
                     CRMCLVDMVGAPKLVPGCSTTVGELPPGKKIEDKYDAVIFTENEKVTTARRAVLEFLL
                     LNHPIDCPICDKAGECKLQDYTYEHGNHESRFAEIKRIPQRKDLGPSIYLYTSRCIMC
                     TRCVRFTEEISGTEELKVNHRGARAEIEVNPGKPLNNKMMGNVVDLCPVGCLIDKNEK
                     FEGRVWNFVHTESVCPGCSKGCNTTLDTMKRQKLNLEELVDKVYRVRPRENMAVNQWW
                     ICDDGRYHLNAQEKLTRILVPHTRKDNKLSETNWTAAMALVTEKIKSAGSGSVAFIAS
                     PYASNEENYVFGKLAKSFGASLDVSKPLYGDTQTFKTDKNKYPFMIESEKAPNYRGAK
                     EVLGLGDSKMEQTIAGKKVVVLLGSHNIELKTKPETLIVISHFEDAVTNQADVVLPGT
                     IFTETSGTFTNKDGLVQKFNKAIQPAGESRPVWQIVLDIASALEYGLYYESVADITKE
                     IKETVSSYSSLDMDKLYPQLAYTNEQMVL"
     gene            complement(12444..12962)
                     /locus_tag="PK754_01695"
     CDS             complement(12444..12962)
                     /locus_tag="PK754_01695"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB08249.1"
                     /translation="MAKAKKKVVKKAAKKVARKKVKALKKTVKKPTEKIPAKGLSPYL
                     CFNGNCREAMEFYRDSIGGKILSLQTFGDAPMDSTPEQKNLVMHSEFKADAVYFMASD
                     AMPGQPVTSGSNVTLSINFTKLAEQEAVFSKLSAGGTIIMPLQETFWGAKFGMFTDKF
                     GIQWMLNCQLKK"
     gene            complement(12981..14282)
                     /gene="nuoF"
                     /locus_tag="PK754_01700"
     CDS             complement(12981..14282)
                     /gene="nuoF"
                     /locus_tag="PK754_01700"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNB08250.1"
                     /translation="MEKVLTKMVGVENSQNIDVYIKHGGYQALPKALKMTPDELIQMM
                     KDAGVRGRGGAGFPTGMKWSFLPKDNPKPRYLLCNADESEPGTFKDRVLMEDDPHQVI
                     EGLVISSFAIQANTCYIYIRGEYVKGALTLRKAIDEAYAKGYLGKNILGSGFNLDITV
                     HRGAGAYICGEETGLIESLEGKRGWPRIKPPFPAVFGAFGCPTIVNNVETLVTPVHII
                     NRGIQWWKDNEPKLYCMSGHLTRPGTYEAKMGTNLLTLINEHCGGIRHGRKLKAVIPG
                     GSSVPVLRAEECNVNMDFDSLAKVGSALGSAGCMVMDETTCMVNALWNLLKFYAHESC
                     GQCTPCREGTHWLEMILGRVETGKGRMEDLDLLLETANNIAGRTICPLGDAAAWPVAG
                     LPDKNKGGDLNGQSFVNKFREEFEYHITNKKCMTTIPHEWN"
     gene            complement(14369..14845)
                     /locus_tag="PK754_01705"
     CDS             complement(14369..14845)
                     /locus_tag="PK754_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNB08251.1"
                     /translation="MTLEFSPENKKEVEWLISRYPTKQAATLPVLHVAQQQFGWISHD
                     VMNLVAKTLDEPVSRVEDVVTFYTMFHQHEVGKYHFQVCHTLGCAINGASQVVDYLEK
                     KCGVKCGEGRSADGKFSIVKVECLGSCGTAPMLQLNDDYHENLTKEKIDALITACK"
     gene            complement(14933..16240)
                     /gene="nuoD"
                     /locus_tag="PK754_01710"
     CDS             complement(14933..16240)
                     /gene="nuoD"
                     /locus_tag="PK754_01710"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HNB08252.1"
                     /translation="MEIDRSEMVEDFKDQSSEETLKKAKEAYKKHVKIGDDDESLSLP
                     GAQLLTLNFGPQHPATHGTLRMVMQLDGETIVNLDPSPGYLHTGFEKLAEHHTFNQFI
                     AVTDRMNYLSPMSNNIGFAQAAEKLLGITLTERAQYIRMIQGELSRIADHLVNIGTHA
                     LDLGAFTVFLYTFRQREFIYDLFEESTGSRLTTSYTRIGGVVRDFPEDHPRHIAQWCK
                     QFKATCTDQVEGLLNHNKIFLDRTQNVGKISAEDAINCGMTGPCLRASGVATDIRKDE
                     PYLKYDEVDFDVPIGTEGDVYERYLVRMEEMKQSIRIIEQCLERMKPGPVKVADHTIA
                     LPDKNKVYGSIEGLIHHFKLIMDQHGIAMPKGEIYDATEAPNGELGWYIVSNGEGTPY
                     KVHVRAPSFIHFGMMSHMAKGGLIGDAVAILGSMNVIAGECDR"
     gene            complement(16294..16815)
                     /locus_tag="PK754_01715"
     CDS             complement(16294..16815)
                     /locus_tag="PK754_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNB08253.1"
                     /translation="MPNNEKNILENNPTLDAVKSKFGESLLKSEVFRNELTLYIKRES
                     IAEIMAFLKNDSDLAYDMLNDVTVVDGLIMEKNPRFQVVYHLRSTKYNRRLRIKAPVP
                     ENDCKIATVSHLWKLADWAEREAYEMYGVTFEGHPDLRRLLTPASFTSFPLRKDYPLR
                     GKGERDVILPEGS"
     gene            complement(16857..17249)
                     /locus_tag="PK754_01720"
     CDS             complement(16857..17249)
                     /locus_tag="PK754_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNB08254.1"
                     /translation="MLFILQDGPVYLNYLPVLVLMALAIIIPIAILILSRLLGPYKPN
                     ERKLSVYESGMPPVGDAHPKFSVKFYLIAMLFILFDIEIVFMYPWAVIYNKWVSVSTF
                     ILIEGLVFIGILLVGYAYAWKKGALEWE"
     gene            complement(17482..19548)
                     /locus_tag="PK754_01725"
     CDS             complement(17482..19548)
                     /locus_tag="PK754_01725"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNB08255.1"
                     /translation="MKRNLLMLAFVVATASLSAGEPNVNPLMAGFNQNIEFNVLTPEH
                     IKTSTDIIIEKSKTALSGIFAIPKEKRSFDNTMLAIDNMYNDYGKVSSNIYLMANAHP
                     DKATREQALASLSVLDKFDNEIALDENLYRAVKEYSESPEAKKLQGFKKKYLEEILRD
                     FERNGFALPKEKRDQLKTMQDRLSDLGITFGRNIAEAADTLFVTEEEMKGLPDDYKSA
                     RKMSDGRYRIDMTYPSYVPFMKYSQSESARKALYVKYNNRSVDKNLHVLRDLVGQRQE
                     MATLLGYKTYGQYRVEDRMAKKPENVWNFESALIDKVKEKARRDYDELLAVKRNYLKD
                     ASASVINPWESSFYNGILMREKYSVDDEKIKEYFELNNVTEGLFKISQKLFDVEFSEI
                     KNPSVWHSDVRMFEVRKKGKVIAHFYFDLFPRPNKYGHAACFGMVKGKSTPNGYQLPA
                     ATLECNFPAPSGDRPSLMYHSAGSASVETFFHEFGHVLHNILTTSELYSQSGTAVPRD
                     FVEAPSQIFENWAWDYDVVKMFAKHYKTGEVLPKAMFDKMVAAKNVGSGIGALQQVFY
                     GMVDFTLHEKWNPKGAETSTDIVKRLQNEITLFPYLEGTNFQASFGHLNGYGAGYYGY
                     LWSEVYAADMFSVFEKNGVMDQKTGLRYRDIILARGGSEDPYALVKEFLGREPNQEAF
                     LKSLGL"
     gene            19792..20259
                     /locus_tag="PK754_01730"
     CDS             19792..20259
                     /locus_tag="PK754_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HNB08256.1"
                     /translation="MNLTEIAIIIHSTQESEYIKGCKETLDRFGLHCEVKVFTPLLDP
                     PELQEFMQKAPERGIKIFIATSSLQSKLAAMIAAYTHLPVIAIPIGKEGFTDRDATIA
                     SVEMPEGAPVAVVSSYPTGGKNAAILAAQILAFSDNHLMDRLKLFKQNGCRFV"
     gene            20270..21001
                     /locus_tag="PK754_01735"
     CDS             20270..21001
                     /locus_tag="PK754_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNB08257.1"
                     /translation="MTRALIVVPTYNEAENIPVLIQRIFALDFSEYQVAPEILVVDDS
                     SPDGTADRVQTIQKNDTRVHLLSRPGKQGLGKAYVAGFSWALERGYEYILEMDADLSH
                     DPSYLKDMFRLIRDNDLVIGSRYINGVNVINWPLKRLLISYFGNMYARMVTGMPIMDA
                     TAGYKCFRRSVLSSMDLTKVSASGYSFQIEMNYRTWLKKFRIIETPIIFYDRTVGYSK
                     MSTNIIREGLLLVWKLRIKHFFGKI"
     gene            21060..22022
                     /locus_tag="PK754_01740"
     CDS             21060..22022
                     /locus_tag="PK754_01740"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNB08258.1"
                     /translation="MNVPVILDFEKPIFDLEAQIADMKSAHANVDITPEIEALQNKLE
                     AMRKEIYANLTPWQKVQLARHPERPRSLDYIERISDGFMELHGDRLFGDDHAIVSGIG
                     RIDGKEVMIIGQQKGKDTKSHVYRNFGMPNPEGYRKAMRLMHMAAKFKRPILTLIDTP
                     GAYPGIEAEERGQAEAIARNLLEMSRLPVPIICVVIGEGASGGALGIGVGDKILMMEN
                     TWYSVIAPESCSSILWRTWEYKERAASELKLTADDLIRFDVVDDKIQEPVGGANRNYD
                     LAAQYLKEAVMHNFAELEKLSVEQLLDQRMEKYSKMGRWGEMSA"
     gene            22038..22436
                     /locus_tag="PK754_01745"
     CDS             22038..22436
                     /locus_tag="PK754_01745"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNB08259.1"
                     /translation="MKITRTTVRVRYAETDSMRVAYHGSYLPWMEVGRTELIRELGYS
                     YKRLESEGIFMPVIRIEVDYLRSVHYDDTVHIDAWIASQEGIRTTIAYICYRDSEPVA
                     RGFTTHAFTDRQNKPVRPPKEFIACLKKGI"
     gene            22415..23038
                     /locus_tag="PK754_01750"
     CDS             22415..23038
                     /locus_tag="PK754_01750"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNB08260.1"
                     /translation="MLKKGYMRIILGFFCFVLSLQVSLHSQNIDARLFKAINKHHSPG
                     WDRFYEFQSRSVKPVYIATPLSFVATGLLRKDTYMRDAGILMTTSAVANLAITYSMKA
                     AINRERPYKALNNVHTVGSTERSASFPSGHTSSAFNMATSLSLAYPKWYVIVPSYAWA
                     GVVGYSRIKMGMHYPGDVLAGALIGAGTSYLTFKLQKPILNTIHKLF"
     gene            complement(23013..23618)
                     /locus_tag="PK754_01755"
     CDS             complement(23013..23618)
                     /locus_tag="PK754_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNB08261.1"
                     /translation="MKKTEAIIFAAGQASRYGDNKLLLPIGDVPAMIYAIRTLCAAGV
                     DRIAVITGAYHDTIKQALIREPVRIFLNPRHSEGMSATICFSVQQIDQSSESILLLPG
                     DVPLFSRQTIKTMMQYGLTHRIVIPRYQGKKGHPVLVDRKIVEECLETGLEMPLYEVI
                     QNHQNEVFYLDSDDQGVVLDIDTREDYDAILSYFKTTYGSY"
     gene            complement(23624..24256)
                     /gene="thiE"
                     /locus_tag="PK754_01760"
     CDS             complement(23624..24256)
                     /gene="thiE"
                     /locus_tag="PK754_01760"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNB08262.1"
                     /translation="MKNNIPFRFYGITDRKRMIESLFLSHLDRAVKAGLRALQIREKD
                     LSPRALYELTDKIRTISATRSVRIFINDRVDIAMALDLDGVHLSTQSMPTYNVRKLVG
                     PDKLIGVSTHDLHEAIHAENEGADFIVIGPIGETRSKPKGHAVLSEKEFEIIREEVKI
                     PIFVLGGVQTNNISHWLQKGAHGAAGISLLMDDENLEERFSNLKSTLGSL"
     gene            complement(24246..25196)
                     /locus_tag="PK754_01765"
     CDS             complement(24246..25196)
                     /locus_tag="PK754_01765"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNB08263.1"
                     /translation="MLSKKLSFYIKIIFTIAFVGYLIFKVDPAMIRADFSSIAWDYFW
                     IGAALTLPNLLIQWLKWQYLVRMIDPDVSRRSVWRSLFAGFSIGLITPGRIGEMGKGV
                     FIDSPQRKLIIGFGVIDKILSQIALAVFGAVSLLYLTEFVFDFNRPTQLIILVFVFWV
                     VVALAVMIFRPSSIRWLIEKLKSWVRFLPWNQHITDVLSAADEFKRHHFWPSLHYALL
                     FQWIVFIQIYCFANAFHSISFWEALSAAAAAMFVKALLPITLMDIGVREGALIFFLGR
                     FGVDAASAFNTSVMIFASNVLLPGLIGVFFVYKFRWSREK"
     gene            complement(25209..26444)
                     /locus_tag="PK754_01770"
     CDS             complement(25209..26444)
                     /locus_tag="PK754_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNB08264.1"
                     /translation="MLNRVALFLFFSLISLQAQNLQKQIDKSRNNMKKIKDEIAQYEN
                     RLTSQAQKEKTEIENLNDINEKIGLLHQLLGELEQGRRLTEERIAELRSILSQTRTEL
                     QNLKGLTSQRLLQTYKHRDEDPLAYILTSDSWTQAFARMKYLKLIAEQDQRDIQSLRT
                     KKEKIETQKKLIEVELLNQQFLISEKKREKANLDQQLARRKNTLSKIRKDKRLLASLI
                     EQRKDDMANLQTIIAALEKKKAEEEALAKKRAEEAAAAAKSGKTKTAGSVKYKEPVYT
                     EKSNFAQYKGRLPYPVPGKIVVSFGNQYNAALGTTTRNTGVDIQSREGAEVHSVAKGK
                     VSLISWLRRLGNTVIIDHGEGFYTVYAHLSEIFVSVDQHVAAGQTIANLDESDEGKAV
                     LHFEIYKDREAVDPAGWLR"
     gene            complement(26437..>27860)
                     /locus_tag="PK754_01775"
     CDS             complement(26437..>27860)
                     /locus_tag="PK754_01775"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNB08265.1"
                     /translation="MKKNQPHILLINPYIYDFAAYDLWSKPLGLLYIAAVLEENGFRV
                     SLIDALDRHDTEMLAWQGRASAKSRMYGDGYFYREIVEKPTVFSHIPRHYARYGFTPE
                     LMRKKISDLNLEQSVDSVLVTSGMTYWYKGVHEIIAMCKELVPQAEVILGGIYATLFT
                     NYAMEFSGADRVFTGESEKTVLAYLCSQSGLEVKKNYKGYDDYPYPAYHLYSKLEYVA
                     MMTSRGCPYSCTFCATHAFTEKFTPRSLEGALHEIINYVNQGIQNITFYDDALFVNSD
                     KHIKRILRGVIENGKNVNFHTPNGLFARLIDAELAKLLVQSGFKTIRLSYETKNPERQ
                     KVLRKVSDKDLESALEYLEQAGFLRHDIVVYLIMGLPDQSPDEVQAGIDYIHSLGAKV
                     SLSSFSPIPGTADWEMARQKYNFPVNEPLLTNKSVYPLRHPGFTSEDFDGLKNYAVEK
                     NRSYPKDNPTLDENHVSDYNVYA"
BASE COUNT         7878 a         6597 c         5707 g         7678 t
ORIGIN      
        1 cggcatgtga aatactcgaa aatgcaagca tacgtttgat atcgcgttgt accaatgcca
       61 tcaggtttcc gccgatcatg ctgatcacac agatccaaaa aagcacttca gaccattcga
      121 tcgctaacgt attgaaggtg tcaatgaata cacgcgcaaa agctgcgaat gcggccactt
      181 tgggaccact cgcgatgaat gctgttaccg gagcgggcga accttgatat acatcgggcg
      241 accacatgtg aaatggaact aacgcaacct taaaaccgaa accaataatt aatagcgaag
      301 tgcccgccaa cgccagagga tcaccgagta aattattcat tgaaagataa ttagcaattc
      361 gtgcaaggtc aaaactaccc gttgctccgt acgtcaatgc aataccataa agaaaaaagc
      421 cggatgcaaa agcgccaagt aaaaaatact tcataccggc ttccagcgaa cgaattttag
      481 accggttaat actgataagg atatataaac ctatggacat gatctcaagc ccgaggaaaa
      541 tcaccatcag gttcatactt gccaccatga tcagcatgcc cgtcgttgac atcagcaaca
      601 ccgaaaagaa ttcgcctact ttatgttcac tcacgtcgtc gtatgtcacg aggcaaacta
      661 gcgcagcgct cgcgagtatg acgatactga aagcatgcgt gtaatagttt agcgttatca
      721 taccgctaaa agctaccatt ttggtatccc acatcgtcat cacatcaaga aatgcgatca
      781 cacatgtcgc aaacgcgaca taacccgccc attttctcag atggggtgta aaaacaccca
      841 tcaataatgt tacgcatgcg ccgatggtca ggatcacctg cggcatgatc gctgttatat
      901 tcgtatccgg aatgactatt tccacaaatc ctccgtaagg tttttatatt ttatgcgccg
      961 cgcaaaacga gtatttcgtt ttcactgcgt ttgatgttat aagctttaca attcgggttt
     1021 tgaatcatga cacgtgacag cggtctggat atcatgtctt ccaattgacg atttattccg
     1081 cgcgcgcctt cccgtttgct gtcgatgtct cgcacgcaca gcgccgcaac atcttgttcg
     1141 atatggatgc gaatatctcg gtcggcaaaa cgtttgacta ttccatccaa cttctgctgc
     1201 gcgatttgta taagcgcttc atcatccaat ggcctaaaca ctataatttc gtcaattcga
     1261 ttcaaaaatt caggtgaaaa atgctttcgc atggattctg tgataccttg cgaaatttgc
     1321 ttgtgaacat cttctgttcc acccgaaaaa ccaagtttct cttttccata aatatgttcg
     1381 gcaccgagat tggatgtcat aatcatcgtc gtatgagaaa acgaaatcac atttccttta
     1441 ccatccgtca tacgaccgtc atcaaaaacc tgcaaaaacg cttcataaat ttttgggtgc
     1501 gctttttcga tttcgtccaa aagtattacc gccgaaggat cttcgcgcac agctttagtt
     1561 aaccgccctt cgtcttcata ccctacaaaa ccgggtgctg ttccgatcag tttggataag
     1621 gcatactgat cattatactc cgacatatca atgcgaacaa gtcttttatc actgccttct
     1681 aacgcttcgg ccaatgcttt agccaattcg gtttttccga cgccggtcgg gcctacaaaa
     1741 agaaaaacac cgtccggtcg ttccggattt acatcaagac cgcttttggt catgcgtacg
     1801 acttcagcga ctttttggat cgcttcctct tgccctatca cccgcatgcg caaacgatct
     1861 tcgagcgaca gcatgcccat catggcgtca atttgaatat tactttcggg aatgcgtgtt
     1921 acttgagaaa ccgcttgtac gatgtgctct tcggcaactt cttttatttc accttcggcg
     1981 cccgaacgcg acagacttgc tttgatcgaa gcgcgctcaa gtaagtcaca ggccttatcc
     2041 ggaaagtaac ggtttttgat atatttttcg gccatatcgt acgtcgtacg gagcatacga
     2101 tcactgactt gcacatgata aaacggttcg atttttccgc gcaaagatcc gagtatattc
     2161 atcgtctcat cacgcatagg ttcaaatact gtaatacgtg taaaacgacg atcaaaagcc
     2221 ccgcttttag ctatgttttt ctgatattct tcgtccgtcg tcgcaccgat caatttgaat
     2281 tccggttgcg tcatcagcgg tttgaacatt tcatccaacc cgcctgtttc atgcgaacta
     2341 taggaaccgg cgcccatcaa tgtgtgcagc tcatcgataa aaataattac tttgttctgc
     2401 cggcaatatt gagccagttg cataaatttc tgctcaagcg ttccgacata tcgtgtaccc
     2461 gccacaacag caaaaacttc cagttcgagc aaacggtaat cttgcaaaat atcgggaact
     2521 tttttttgtg ccagcaagtg ggcaaggcca ttcaccaaaa ccgttttacc gacgcccggt
     2581 gcgcctacga tcaacggatt agctttattc cgacggcaca aaacttcgat cagttccatc
     2641 aactcatgcg tgcggccaaa aatgttttcg atcttccctt ctttggccaa ttgcgtcagg
     2701 tcgcgtaaaa aattcggaag cgccggcgcc accgtttcac ccaaaacgcc cgtaccggtc
     2761 gtaaccggtg tttcggttac attttccggg attgccgtcg gcgtataatt ggggtccatc
     2821 tctgtcagaa gttcatcaaa ccatttatac gttggatcaa actgtttgcc ctcattcaat
     2881 gtctttcggg ccagatcatc cttgcctaac ttgtgatagg ctaaggcgag gtaataatag
     2941 ccgtacgaat tcttattttc gatggcaaat gattttttta aaacttctat agcttcctgg
     3001 tagcgttgca aatcgcagaa tacacgcccc aattcgcgca tgactcgtgc atttttaggc
     3061 tcgagttcga caaaacgttc aaacgctttt acggcttcat ccaatcgccc cgtattcatc
     3121 aaactatatc cataataatc gtaaacaaac gaatcggtga ttttattggg atacgttttt
     3181 tcgacgagtt cgataagttg tttatcgtct ttcagatcat agtagagaat ttcaaaaata
     3241 tgatgcatcg cccaattata gtaggacaca tccgtgcgct gatactcttt cattacctca
     3301 atagcttttt gaggattatt gagtttgcga tacatttctt tggtttcgta cgctgcatta
     3361 ctgtctttat tattaagctt aagaatgcgt tcaaaatatt tcagcgcttc cgcccaattg
     3421 cttttttctt tatacagttt tccgagatta cgcaacgtgt ctaaattgga ttcttccgat
     3481 gcgaggctgc gtttgtacat ttcaagcgct tgttcgggtt tattttgttt ttgataaacc
     3541 tccgcaagac ggtttaatgc agaaacattg gaaggctgcg atgaaatgac ttgctgaaac
     3601 gcggtttccg ctttttcgta attcatcttt ccgagataca gattgcccat cgcgaagtgg
     3661 attatccgta cctccgcatg ttgtttgcga agctcttcgt aggtggattc caccataaca
     3721 agatctatcg catcatcaat caattgatca aagtttttga tatacgcacc gctaagggcc
     3781 attgccataa gataggagcg tgcactgagc gtagattgat tttgtttgtc atacacttca
     3841 gcgatacgcg catgatggcg tgactccata ggatccaacc gaacagcttc ttgggcatgt
     3901 ttgaatgccg cctcaagcat accgatctgc tggcatgtct ccgaaagggc gtgatgattc
     3961 cacgcatctt gcggattgat ctgtacggca cgttcgcagc acgcaagcgc tagtgcatcc
     4021 ttatgcgtcg catgcagttc cataccgatc atacgcagcg ctttatcatc caaaaaattg
     4081 cgatgtataa ggtgatgaat aagaagcccg acttcttcaa aacgttcttt ttcgatgccg
     4141 gtgcggattt ctttgacaca caacccgtga tcttccggta cgataaaact tttcaaatcc
     4201 acactaaaca agtaacgaca atgcgggcat gcggccggct gcgtatccgc cgtcagttct
     4261 ttaccgcaat tggggcacaa tgtggttagt gaagattgca ttggaagtta ctactgagtt
     4321 tctagttgtt caaagtttta tacaaaaaag gttcgcagca tgcgcgaacc ttttcataat
     4381 tggtcgaaat cactgcttca tgcgctatcg tcatttgttt atcattcgca caaagccgca
     4441 tgcaaaagat attagtacgg tatcggctga ttggtcatca tcgggccgct attttttttg
     4501 gttttggcct gttcgacagc cgttttctga ttattcagat acttatgcat ggttgcttct
     4561 gttttttcgg tcagaatttt cggatagaat cccaacagga aaatcagaat aagaaacggt
     4621 gtgacaagtg catattcacg tactgaaaga tcgggaagtt ttttgttttc ctcgtgaacg
     4681 atctcgccaa agcagacgcg ttggtacatc caaagaagat aaacagcgcc caagatcacg
     4741 cccaacatag ccagcgtcgc atagaggcgg gcatggccta tggacgaaat aaatgttccg
     4801 atcagaacaa ggaattcacc gataaacccg ttggttcccg gaagacccaa tgatgaaaaa
     4861 caaacaatca taaaaataac tgaaaaggcc ggcatgactt tccacaaacc gccaaatttc
     4921 gcaatttcgc gtgtgtgacg acgatcgtat aacataccga tcataagaaa gagtgcgctg
     4981 gttgatatac cgtgaccgat cataatcatc atcgcgccct gccaaccttg aatcgtaaaa
     5041 gcaaaaatac cgagcataac aaaaccaagg tggcttacgg atgaatacgc aaccaatttt
     5101 ttggcgtcgg tttgtaccat agcgacaagt gcgccataaa taataccgat gatcgctacc
     5161 accataatca cgggcgtaaa atcattggcg acttcaggaa aaaacggaat acagaaacgc
     5221 ataaaaccgt agggccccat ctttaacaag atcgcggcta gatccactga gcctgccgtc
     5281 ggcgcttcga cgtgggccaa gggcaaccat gtatgaaaag gaaacatcgg aactttaact
     5341 gcgaaggcga gtgcaaacgc aaggaataac caatattgag tctcgtatgg taattgtaat
     5401 tttgcaaaat catcaacagc aaacgaatgc atggagagta cggttccatt atccactact
     5461 gcaccggttt ggaaatacat ataaataatc gcgacgagca tgaacaagct gccggccata
     5521 gtatatatga agaacgtcac cgcggctttg atacgatgat cgtggcccca aataccgatg
     5581 atgagataca tcggcaccag catagcttcc cagaacacgt aaaacaaaag catatcccgc
     5641 gcacaaaaaa cacccaccat tccggcatcc agtaaaagca acgaaatcat gaaaccttta
     5701 acgtgaaagt gaatgctttt ccatgaagca atcacagcca aaggcatcat aaaagtcgtc
     5761 agcatgatca aaagcatcga aagaccgtcc acgcccaaag tatagtaggc cccccaatca
     5821 acaatccatt ttgctttata cgtaaactgc atttccggat tgctgaagtc gtaattcatg
     5881 tagatcggta tggacaaaac aaacacgagc gcagaaaacg caaatgttgt tgctttgatc
     5941 aagttttcgt tgcgtcggtc aatgaaagaa ataacgacga caccgaccaa cggcataaaa
     6001 atcagaagtg aaagcgcaag cgaattataa ttttccatgg aaaagcctct atcaaatcaa
     6061 cgaacttttt tcagaattac ctctagcgca tgatatagta aataatcagc gccacaccca
     6121 gcgaaataaa aaatgcatac gtctgaacat aaccggtttg gaataatcga aggatgcgtc
     6181 caaataaatc cagcgtaaca gctataagat atatcaaccc atcaatcaat ttgttatcaa
     6241 ccaagcgcag aaatactttt tccgataccc attttgtagg ttccacaata aatttatcat
     6301 agaactcatc tatgtaatat ttattgagca gcgttttgta gattggttgg tagtctgctg
     6361 cgattttttc agcgctgact ttcttagcca tatagatcac ccacgctata ccgataccta
     6421 tgacaccggc caaaaccgat atgaccatca gcgtcatatc attgaagccg tgatgctcaa
     6481 cgggcgccgc atgcgtaccg accgacggtt caagaaaatg cgcaagccaa ttatgaccac
     6541 ctagaaccgg aggaataccg atgagaccgg caattgccgc aaacacagct aaaatcataa
     6601 gcggcacggt catagtcgaa gtagattcat gcaagtgttc ttttgcagct tccggagtcc
     6661 ggctttctcc aaaaaatgta aggaatacta atcgtgacat gtaaaatgcc gtcatcatcg
     6721 caccgagcac accaaaccag aaaacgccat tgtatccttt atgataagcg gatgccaaaa
     6781 tttcgtcttt actaaaaaat ccggaaaaag gcggtaatcc gcagatagct acccaaccga
     6841 tgagaaacgt aatggcggtt attttagctt tggaaaaaag tccgcccatc ttgcgaatat
     6901 cctgaggatc ttcatggatt ccggctttgt ggtatgcgtg ttccatcaca tgaatgacac
     6961 tacccgaacc aaggaacatc agcgctttga aaaaagcgtg tgtcagaaga tggaaaacac
     7021 cggccgcata agcgccgaca ccgacaccga taaacatata tccaagctga gaaaccgtag
     7081 aatacgccaa aactttttta atatccgttt gtgtaaaacc gatggtcgcc gcaaacaatg
     7141 cggttgccgc gcctacatac gtcaccaagt cgcccgccga catgcccata atgtatcccg
     7201 ccgggccgat gtcaaatgaa aatatcggac tgcaacgcgc taccatgaat acaccggcag
     7261 taaccatcgt cgcggcgtga atcaaagctg aaaccggtgt cgggccttcc atcgcgtccg
     7321 gaagccaaac ataaagaggt atctgcgccg atttgcccat cgcgcctaca aacagaagca
     7381 taccaatggc cgtcatcgtc gtgccgagcg cataaggatc aactttgtgt atggcgtcaa
     7441 aaagcgtttc aaaatgaatc gttccaaacg tagcaaacac caaaaatata cccaatccaa
     7501 agccaaaatc gccgatacgg ttgacgataa acgctttttt tccggcatct ccggcgcttt
     7561 tctttttata ataatacccg atgagaaggt aggagcacaa acctacggct tcccagccga
     7621 aaaacataac ggcaaggtta tttcccatga ccagcatgag catggaaaaa ataaacagat
     7681 tgaggtacgt aaaaaaccga ggacgatccg gatcgtgttc catgtatcct aacgaataga
     7741 catgaatcaa gaaaccgata ccggtcacaa tcaaaagcat tacgccggtc aaaggatcaa
     7801 tcaaaaaacc aaacgtgatg cgcaaggtat caacaataat ccaatccaag taatcaatgt
     7861 tgtacttttg accacccagc acctgaataa aaacatacaa cgacaataaa aatgaaataa
     7921 aaaccaatcc ggaagcaaga tatccggccc agggtttggc atgatgatgc gcgtggcctt
     7981 cctcatgacc ttcggtgtga tgacttccct gcccttcgat catgcggcca aaaaacacat
     8041 tgaataccgc gccggccagc gggatcaggg gaatcattag aataatcatt tccatataac
     8101 aaccatctcc ataaaatttc cgtccaccgg tcggacgcta ctaaccttgc atcatatgca
     8161 tttcatccac attgatcgtg tctttcttac gatagatcgc tacgatcaat gccaaaccta
     8221 cagcgacttc tgctgcagca accgtcataa ccaaaaaaac cgcgatttgt ccgtccatcg
     8281 aatccaaata ccgtgcaaag gctaccatcg tcagattcac ggaattgagc ataagctcga
     8341 tacacataaa tacgatgatc gcgttgcggc gtgtcagtac gccaacaacg ccaagcgaaa
     8401 ataatatcgc tgttactatc aaataatgag agaggggaac catatttgct ccagcactta
     8461 ttgtttacgt ttggaaataa taatcgcgcc gaccataccc aaaagaagaa gtatcgaagc
     8521 gatttcgaaa ggatacaaat actgtgacat cagtaccgag ccgatgactt cgacgtttcc
     8581 ggcctcacgt attttttcga tcggataaat tccccgatca ccgagattgt acgtaacttt
     8641 cgttgcaaga ccgatttgta ccagtatcgc aaaagccagc atgatcgaga tatacttcat
     8701 aggtccggcg tattcggtca gatgtgcatc acgacccaaa ttgagcaaca tgatcacaaa
     8761 aaggaataga accattatcg cgccggtata taccaatact tgaattgcgg ctataaattc
     8821 ggcttccagt gtaatgtata acacggcaat acagaagaac acccccacca taaaaatcgc
     8881 actataaacg gggttttcca tcgtcagcat catgatagcg cataggacag caatcgcagc
     8941 aaaaatataa aacaccggat ttaccatcac atattgcaac atattttcca tagtacctct
     9001 aaactttatt ctgatcctcg gatgatatac atcagccgca gtatcccgtc atctttttag
     9061 tgaaccgatc cgtgttcgtc agagtgtttg acgtctttca cggtaaaata actcgaattt
     9121 ttcatttgtt tatgcaaacc gtcgcgccgt tgtgcacgat ccgggtgacg cttgatgtat
     9181 tcatcatata cacccaaaag acgttcctta ttgtaaatga aatccgcgcg cgtgtaatct
     9241 gcaagattat gcatatccgt catatcaatc gcatcttcgg gacaagcttc aacgcacata
     9301 ccgcaataaa tacaacgcaa ttcgtcaatt tcgaaaactt taggatgttt ttcgcgattt
     9361 tcgtgcccgt ctttccatgc atcaccgctg tcgttcgcta cgatacgaat acaatcggcg
     9421 gggcatgccg tagaacacat ttcgcatgct acgcattttt cgcgaccgtt ttcatcccgc
     9481 gtcagcatgt gcagaccgcg ataattcgga ggaagaactt ttttatcctc gggataactg
     9541 agaacatcgc gttttttgaa gagatgctta aatgtaatac ccaacccttt taagatcggt
     9601 attacatacg tattgtccag ccaactctta ccgcggggtt cagaaacatt ttttacattc
     9661 acagccatat tgttataaac cttgcgttat accttttcta tagcgttata cgaaagccag
     9721 ccaaatagcc gtaatcaaaa tattagccag cgccaaagga atcatttttt tccaaccgag
     9781 attcatcaac tgatcataac ggaagcgcgg aagcgtccag cgtacccaca catagaaaaa
     9841 catcaggaaa agcactttaa atgcaaaacc gccaagattg atcagatcgc gtgtgagcgg
     9901 agaaaacccc atgtcattta caaaaggaat atgccaaccg cctaaaaaga ggcatgtgat
     9961 cataatcgag tttgtaagca tattgcaata ttcgccaaga taaaaaagac cgaatttcat
    10021 cgaagaatac tccgtgtgat atccggcaac aagctctgcc tccgcttccg tcaaatcgaa
    10081 cggcgtacga ttggtttccg caaaagcggt cgcaataaat aacaatgctg caaggggttg
    10141 aaatacgata ccccacatgt ggttttgaat ctgaaaattg acaatatctt cgatcttaag
    10201 cgaaccgaca accagcacca tactcatgat cgccataccc atcggtaatt cgtagctgat
    10261 catctgtgcg gccgaacgca taccgccgaa cagcgagtat ttattattgg atgcccatgc
    10321 accaaatgta atcgcgtaaa cactcaacga agaaaccgcc aacacataaa gtacgccgat
    10381 gttggtttgt ccgatctgga tccagatttc acgcccgccg acggtgataa aaccccaagg
    10441 aatcacgccc acgacaaaaa acgcaggaaa caccatcaat gcgggtgcag ctacatacaa
    10501 ccatttattg acatgcaaag gtatgatatc ttctttggac atcatcttaa gcgcatcggc
    10561 gatcggctga aaaagaccca gcggaccgac acgattaggg cctacacgat actgtaccca
    10621 tgcgctgaca cggcgttcga cgatcaacat gatcggcact atcgcaagat agatcgccag
    10681 catcaatccg atcactaagt acggtacaaa aggtaaaaac gcatccatag tcaaatactc
    10741 gttataaatg aaaattctat aataccattt gttcgttcgt atatgctaac tgtggataaa
    10801 gtttatccat atccaagctg ctatacgacg aaacagtttc tttaatctct ttggtaatgt
    10861 ctgcaacgga ttcgtaatac aatccgtatt ccagagccga agctatatcc aaaacgatct
    10921 gccacactgg gcggctttca ccggcgggtt gaatggcttt attaaatttc tgaaccaatc
    10981 cgtctttgtt tgtaaacgtt ccgctggttt ccgtaaaaat agtcccgggt aacacaacat
    11041 cggcctgatt ggtaacggcg tcttcgaaat ggctaatcac gatcaatgtt tccggcttgg
    11101 ttttcaactc tatattatgg ctgcccaaaa gaactactac ctttttacct gcaatggttt
    11161 gttccatttt tgaatcgccc aaaccaagaa cttcttttgc accacggtag ttcggagctt
    11221 tttccgattc gatcataaag ggatatttat ttttgtccgt cttaaatgtt tgtgtatcgc
    11281 cgtataaggg cttgctgaca tccaacgaag cgccgaatga tttggctaat ttaccgaaca
    11341 cataattttc ttcattggaa gcataagggc tcgcgatgaa agccaccgat ccggaacctg
    11401 ccgatttgat tttttcagta accagcgcca tagcggccgt ccaattggtt tcactcagct
    11461 tgttatcttt gcgtgtatgg ggtaccaata tacgtgtcaa tttttcctga gcattcagat
    11521 gataacggcc atcatcgcaa atccaccatt gattgacggc catattttcg cgcgggcgca
    11581 cacggtaaac tttgtccacc agttcttcaa gattgagttt ttggcgtttc atggtatcca
    11641 gcgtggtatt gcaaccttta ctgcaaccgg ggcagacgct ttcggtgtga acaaaattcc
    11701 acacacgtcc ttcaaatttt tcgtttttgt cgatcaaaca tccgaccggg caaagatcta
    11761 cgacattgcc catcatttta ttattcaacg gcttgccggg atttacttcg atttcagcgc
    11821 gggcaccgcg atgattaact tttaattctt ctgttcctga aatttcttcg gtaaaacgta
    11881 cgcaacgtgt acacatgata caacgggacg tatagagata aatcgaagga ccaagatctt
    11941 ttctttgcgg aatacgtttg atttcagcaa atcgcgattc gtgatttcca tgttcatacg
    12001 tataatcctg caatttgcat tcaccggctt tatcgcagat cggacaatcg atcgggtgat
    12061 tgagcaacaa gaactccaga acagcccggc gcgcagtagt gactttttcg ttttcagtaa
    12121 aaattactgc atcgtattta tcttcgattt tttttccggg cggtaattct ccgaccgtcg
    12181 tagaacaacc cggtacaagt ttcggagcgc cgaccatatc caccaaacac atacggcaac
    12241 ttgctacgat tttcatgccg ggatgataac aataataagg aatctctatg cctatgcgtt
    12301 tcgcggcgtt gaggatggtt tccccacgct gcatttccaa ttcctgtccg tcaattttga
    12361 ccagcgccat tatctgtttc ctttaatatc gcaaaattct tcgccgcgtt tggacagatc
    12421 caaaccaagg cgtttacaaa aattcatttt ttcaattggc agttgagcat ccactgaata
    12481 ccgaatttat cggtaaacat gccaaactta gcgccccaaa atgtttcctg aagcggcatg
    12541 atgatcgtac ctcccgcaga taacttacta aataccgctt cttgttcggc cagttttgta
    12601 aaattgatgc taagtgtcac attggaaccg cttgttacgg gttgtcccgg catcgcatcc
    12661 gaggccataa aatacacggc gtcagcttta aactccgaat gcatcacgag atttttttgc
    12721 tccggcgtac tgtccatcgg agcgtccccg aaagtttgaa gcgaaagtat tttgccgccg
    12781 atactgtcac gataaaattc cattgcttca cggcagttgc cattaaaaca aagatacgga
    12841 ctcaatccct tggccgggat tttttccgtc ggttttttca ccgttttttt taaggctttc
    12901 acctttttac gggctacttt tttagcggct tttttgacaa cctttttctt cgcttttgcc
    12961 atggctttat tcgcagtttt ttaattccac tcgtgaggaa tggtggtcat acacttctta
    13021 ttggtaatat gatattcaaa ttcttcgcgg aatttgttca caaaactttg gccgttgaga
    13081 tcgccgcctt tattcttatc cggcaatcct gccacaggcc acgccgcggc atcgccgagc
    13141 ggacaaatcg tgcgtcctgc aatattgtta gctgtttcaa gcaatagatc caagtcttcc
    13201 atgcggcctt tgccggtttc tacacggcca agtatcattt ccaaccaatg tgtaccttcg
    13261 cggcatggtg tacattgccc gcaagattcg tgcgcataaa acttgagaag attccacaac
    13321 gcattgacca tacacgtcgt ttcatccatg accatacaac cggccgaccc taaagctgaa
    13381 ccgactttag ctaacgaatc aaagtccata ttgacattac actcttcagc acgaagaacg
    13441 ggcacagacg aaccgccggg aattaccgct ttgagtttac ggccatgacg aataccgccg
    13501 caatgttcgt tgatcaaggt aagcaaattg gttcccattt tagcttcata cgtaccggga
    13561 cgagtgagat gaccgctcat gcagtacaat tttggttcat tatctttcca ccactgaatg
    13621 ccacgattga tgatatgtac cggcgtcact aatgtttcga cattgtttac gatcgtcgga
    13681 catccgaaag caccgaaaac agccggaaat ggcggcttga tacgtggcca gccgcgttta
    13741 ccttcgaggg attcgataag accggtttct tcaccgcaaa tataagcgcc tgcgccacga
    13801 tgaactgtaa tatccagatt aaaaccgcta ccgagtatgt tttttccaag gtacccttta
    13861 gcgtaagcct cgtctatcgc tttacgcaaa gtcaaagcgc ctttgacata ttcgccgcga
    13921 atatagatat aacatgtgtt cgcttgtatc gcaaaagatg agatcacaag cccttcgatc
    13981 acctgatgcg gatcgtcttc cataagaacg cgatccttga acgtacccgg ctcagattcg
    14041 tccgcattgc aaagaagata acgcggtttg ggattatctt tgggtaaaaa actccatttc
    14101 ataccggtcg gaaaaccggc gcctccgcgg ccgcggaccc ccgcatcttt catcatttgg
    14161 atgagttcgt ccggggtcat ttttaaagct ttgggcaaag cctgataacc gccgtgcttg
    14221 atatacacat caatgttttg ggagttttcc acgcccacca tttttgtcag aactttttcc
    14281 atgaacgata tccttttaca ctgccctaag gcagcacatc ttatatacta tgaagttcgt
    14341 aaacgcgctc tactttcgtt ggtatgtctt atttacaggc agttatcagc gcgtctattt
    14401 tttctttcgt taggttttca tgataatcat cgttgagttg taacatcggc gccgtaccac
    14461 acgaaccgag gcattcgact tttacgatgg aaaactttcc atcggccgaa cgtccttcgc
    14521 cgcattttac gccgcatttt ttctccaaat agtccaccac ttggctggca ccgttgattg
    14581 cgcagcctag agtatggcat acctgaaaat gatatttacc gacttcgtgc tggtgaaaca
    14641 tggtataaaa cgtcaccacg tcttcgacgc gcgataccgg ctcatccagc gtctttgcca
    14701 caaggttcat cacatcatgg gaaatccagc cgaattgttg ttgggcgaca tgcagcaccg
    14761 gaagagtcgc cgcttgtttg gtgggatagc gggaaattag ccattccact tcttttttat
    14821 tttccgggga aaattctaac gtcataatcc aaattcgttt ggttaaatcc gtatccgttt
    14881 tgttcaagtg cgcaacatgc gtgtgcacac tttatatcaa actgaaatct ttttaccgat
    14941 cacattcgcc ggcgataaca ttcatactgc ccaaaatagc cacggcatca cctatcaatc
    15001 cgcctttggc catgtgggac atcataccaa agtgaataaa agaaggcgcg cgcacgtgca
    15061 ctttatacgg cgttccttcg ccgttactta cgatgtacca acccaattcg ccattgggcg
    15121 cttcggtcgc atcgtatatt tcacccttag gcatcgcaat gccatgttga tccatgatca
    15181 acttgaagtg atgaatcagt ccttcgatgg aaccgtatac tttattttta tcaggtaatg
    15241 cgatggtgtg atccgccact tttaccgggc cgggtttcat gcgttccaaa cattgttcga
    15301 tgatacgaat tgactgcttc atttcttcca tacgcacgag gtagcgttcg tacacatcac
    15361 cttccgttcc gatcggcaca tcaaaatcca cttcatcgta tttaagataa ggctcatctt
    15421 ttcggatatc cgtcgcaacg ccgctggcac gtaaacaagg tccggtcata ccgcaattaa
    15481 tcgcgtcttc agcggaaatt ttcccgacat tttgcgtacg atccagaaaa atcttattgt
    15541 gattgagcaa accttccact tgatccgtac acgttgcttt aaactgttta caccactgtg
    15601 cgatatgacg cggatggtct tcgggaaaat cacgcacgac accgccgata cgcgtataac
    15661 tggtggtaag tcgtgaaccc gtggactctt caaacagatc gtagataaat tcacgttggc
    15721 gaaacgtata aagaaatacg gtaaacgcac ctagatcaag cgcgtgcgta ccgatattga
    15781 ccaagtgatc ggcgatgcgg gaaagttcgc cctgtatcat gcgaatgtat tgcgcccgct
    15841 ccgttagggt gataccgaga agtttttcgg cagcttgcgc aaaaccgata ttattggaca
    15901 tcggcgagag gtaattcata cgatccgtga ccgcaataaa ctgattgaac gtatgatgct
    15961 ctgccagttt ttcaaaaccg gtgtgcaaat aaccggggct tggatccaga ttgacgatgg
    16021 tttcgccgtc caactgcatg accatacgca gtgtgccgtg cgtagccgga tgctgcgggc
    16081 cgaaattgag cgtaagaagc tgtgcgccgg gaagagataa tgattcgtca tcatcaccga
    16141 tcttaacgtg ttttttatac gcttctttag ctttttttag agtttcttct gaggattgat
    16201 ctttaaaatc ctcaaccatt tctgaacgat ctatttccat acttaccttt ccaacgcctc
    16261 tatggaaaaa atttcaaaat aaattcaaaa aaactagctg ccttcgggca agatcacatc
    16321 gcgctcgcct ttaccgcgta aggggtagtc tttgcgcaaa ggaaaacttg taaacgaagc
    16381 aggtgtcaaa agacgacgaa ggtcggggtg cccttcgaaa gtgacgccgt acatttcata
    16441 cgcttcacgt tcagcccaat cggctaattt ccaaagatga gataccgtgg cgattttgca
    16501 atcgttttcc ggcaccggcg ctttgatgcg aagacggcga ttgtatttgg tcgaacgtaa
    16561 atgataaaca acctgaaagc gtggattttt ttccatgatc aatccgtcca cgacggtcac
    16621 atcattgagc atatcgtacg ccaaatcgga atcgttttta agaaatgcca tgatttcagc
    16681 tatggattcg cgtttgatgt acagagtgag ttcattacga aacacttccg atttgagcaa
    16741 tgattcgccg aatttggatt taaccgcatc cagtgtcgga ttgttttcca aaatattttt
    16801 ttcgttgtta ggcataatgt ttctcagtgc gattgatgtt atctagttca ggggttttat
    16861 tcccactcca gtgcgccttt tttccacgca tacgcatacc caacgagcag aataccgata
    16921 aagaccaatc cttcgatgag aataaaagta cttaccgaca cccacttgtt gtaaatcacg
    16981 gcccacggat acataaagac aatttctata tcaaaaagga taaaaagcat ggcgataaga
    17041 taaaacttca ctgaaaactt agggtgggca tccccgacag gcggcatacc ggattcataa
    17101 accgaaagtt ttcgttcatt aggcttgtag ggaccgagaa gccgggatag tatcaatatg
    17161 gcaatcggga taataatagc caatgccata agtacaagaa ccggaagata attgagatag
    17221 acgggcccgt cctggaggat gaagagcatg ggaaacctcg tgctgaaaaa gctctatttt
    17281 taaatcaaat ttttggaaag caacatttta aaaagcggac aattttaaga attttgctaa
    17341 taaagtgcaa cagatatttc ccaatacgta agtagataaa aataaaaagg gttacccgaa
    17401 aagacctttt gtgtcataac gggtaaccct atcttctcta tatagcttct ctattatata
    17461 ggttagatct actaacttac tttatagacc cagtgatttc aaaaaagctt cctgattcgg
    17521 ttcacgcccc aaaaattctt ttaccagcgc atacggatcc tccgaaccgc cgcgcgccag
    17581 aataatatct ctgtaacgca agcctgtttt ttggtccatc acaccgtttt tttcaaaaac
    17641 ggaaaacata tcagcggcat atacttcaga ccaaagatat ccgtaataac cggctccgta
    17701 tccattgaga tggccaaatg aagcttgaaa attggtccct tcgaggtaag gaaaaagagt
    17761 gatttcgttt tgcaaacgct tcacgatatc cgtagacgtt tcggcgcctt tgggattcca
    17821 tttctcatgc aacgtaaaat ctaccatacc ataaaaaacc tgttgtaaag cgccgatgcc
    17881 ggaaccgaca tttttggcgg ccaccatttt atcgaacatc gctttgggca atacttcgcc
    17941 ggttttgtaa tgcttggcga acatttttac cacatcataa tcccatgccc agttttcaaa
    18001 aatctgtgaa ggcgcttcga caaagtcgcg cggtacggcc gtaccggatt gtgaatacaa
    18061 ttccgatgtt gtcaatatgt tgtgcaagac atgcccgaac tcatgaaaaa acgtctcaac
    18121 cgaagcgcta cctgccgagt gatacatcag cgaagggcga tccccgctcg gtgcgggaaa
    18181 attacattcc aaggtcgccg cagggagttg gtaaccattg ggggttgatt tacctttaac
    18241 catgccaaag catgcggcgt gaccgtattt attgggtctc ggaaaaagat caaaataaaa
    18301 atgcgctatt acttttcctt ttttccgcac ctcaaacatg cgcacatcgc tgtgccaaac
    18361 cgacgggttt tttatctctg aaaactccac atcaaaaagt ttttgcgata ttttaaacaa
    18421 cccctcggta acattgttca gttcaaaata ttctttgatc ttttcatcat caacgctgta
    18481 tttctcacgc atcagaatac cgttgtaaaa actcgattcc cagggattga taacagacgc
    18541 ggaagcatct ttgaggtaat tgcgctttac agctaataat tcatcgtagt cacggcgcgc
    18601 cttttcttta actttatcaa tcaatgcgct ttcaaaattc caaacatttt ccggtttttt
    18661 tgccatacga tcttcgacac gatattgtcc gtacgtttta taacccagta gcgtagccat
    18721 ctcttgccgc tgaccaacca aatcacgaag cacgtgaaga tttttatcaa ccgagcggtt
    18781 attatacttg acatacaatg ctttacgtgc ggattcggat tgactatatt tcataaaagg
    18841 cacatacgac ggataagtca tatctatcct atagcgtccg tcggacattt tacgtgcgct
    18901 cttataatca tccggtaagc ctttcatttc ttcttcggtt acaaaaagtg tatccgcggc
    18961 ttccgcgata ttacgaccga aagtgatacc caaatcggaa aggcgatcct gcatggtttt
    19021 caactggtct cttttttctt tgggtaaagc aaaaccgttt cgttcgaaat cgcgaagtat
    19081 ttcttcaaga tatttctttt tgaatccttg gagtttttta gcttcaggac tttcggaata
    19141 ttctttcacc gcgcggtata aattttcatc cagcgctatt tcattatcaa atttatccaa
    19201 tacgctaagg ctggctaatg cttgttcgcg cgttgcttta tccgggtggg cattggccat
    19261 aagatagata ttagatgaaa ccttgccgta gtcgttgtac atattatcaa tcgcgagcat
    19321 ggtgttgtca aacgagcgtt tttctttggg aatggcaaaa atacccgata acgccgtttt
    19381 ggatttttcg atgatgatat cggttgaggt tttgatgtgc tccggtgtca aaacattgaa
    19441 ttctatgttt tgattaaacc cggccatcag cggattgaca ttgggttcac ccgcactaag
    19501 cgatgccgtc gcgacgacaa acgccaacat taacaaattt cgcttcatga tcactccttt
    19561 ggtatattta ggtatattta aaataagaat agattccgat gatatcgggt accgatcata
    19621 cgaaaagata ttcataaagt tccggttatt ttagaaaaat ttgttttaac aaaattacaa
    19681 actcatctat gtctcaaaca aaagcatgta taaagatgcc ttaaaacttt tgtattgtag
    19741 tttacgtagg gtatgttaat ttttgcccta agtttattca aaagtcatat tatgaatctg
    19801 accgaaatag ctattattat tcattcgaca caggaatcgg aatacatcaa aggctgtaaa
    19861 gaaactttgg atcgtttcgg attgcactgt gaagtcaaag ttttcacgcc attactggat
    19921 ccgccagagc tgcaggagtt tatgcagaaa gctccggagc gaggcataaa aatttttatt
    19981 gcaaccagta gccttcaatc taaactggcg gccatgatcg cggcttacac acatcttcct
    20041 gtgattgcca ttcccatcgg caaagaaggt tttaccgatc gggatgctac catcgcgtct
    20101 gtcgaaatgc cggaaggagc gccggttgca gtcgtttcat cgtatcctac cggcggcaaa
    20161 aatgccgcta ttttggccgc acagatttta gcattctcgg acaatcacct gatggatcgt
    20221 cttaaactat ttaagcaaaa cgggtgccgc tttgtataag gaacaatcta tgacgcgcgc
    20281 gctgatcgtt gttcccacat acaacgaagc agaaaacatt cctgttttaa tccaacgcat
    20341 ttttgcattg gatttttcag agtatcaggt cgcaccggag atattagtcg tggacgatag
    20401 ttcgcccgat ggcacggcag atcgcgttca aaccatacaa aaaaacgata cacgggtaca
    20461 tcttttatct cgtccgggta aacaaggtct cggtaaagct tacgtggcag gattctcttg
    20521 ggctctggaa cgcgggtatg agtacatact cgaaatggat gctgatcttt cgcatgaccc
    20581 aagttatctg aaagatatgt tccgtcttat tcgcgataat gacctggtga tcggttcacg
    20641 ctatataaac ggcgtaaacg taatcaactg gccgctcaag cgcttgctca tcagctattt
    20701 tggtaatatg tatgcgcgta tggttaccgg tatgccgatc atggatgcta ctgcaggtta
    20761 taaatgcttc cgtcgttcgg tgctatcttc gatggatttg accaaagtca gtgccagcgg
    20821 ttattcgttt caaatcgaaa tgaattatcg aacatggtta aaaaaattta gaatcataga
    20881 aacacccatc attttttacg accgcaccgt cggatattcc aagatgtcca ccaacattat
    20941 tcgcgaaggt ttattgctcg tttggaaatt gcgaataaaa catttttttg gtaagattta
    21001 acccatttga cggtttgcgt atagtcataa gcaaattttt tttgtgaatc aggaggagaa
    21061 tcaacgtgcc tgtaatattg gattttgaaa aacccatctt tgatctcgaa gcgcagatcg
    21121 ccgacatgaa gtcggcccat gccaatgtgg acataacacc ggaaatcgaa gcgctgcaaa
    21181 ataaacttga agcaatgcgt aaagagattt acgccaacct cacgccttgg cagaaagtgc
    21241 aactggcgcg tcatcccgag cgcccgcgat ccctcgacta tatcgaacgt atatccgacg
    21301 ggtttatgga attgcacggg gaccgccttt tcggagatga ccatgcgatc gtcagcggaa
    21361 taggacgcat tgacggaaaa gaagttatga tcatcggaca gcaaaaagga aaagacacca
    21421 aaagccatgt ttatcgcaat tttggtatgc ccaatcccga aggttatcgt aaagccatgc
    21481 gcctgatgca catggccgcc aaatttaaac gaccgattct taccctgatc gatacgcccg
    21541 gtgcgtatcc cggcatcgaa gccgaggaac gcggacaggc tgaagccatc gcacgcaatc
    21601 ttttggaaat gtcacgcctc cctgtaccga tcatttgtgt agttatcggt gaaggcgctt
    21661 ccggaggtgc attgggtatc ggtgtcggcg acaaaattct catgatggaa aacacatggt
    21721 actctgtcat cgctccggaa tcctgttcca gcattttatg gcgcacatgg gaatataaag
    21781 aaagagccgc atccgaactc aaacttacag cggatgatct catccgattt gatgtcgttg
    21841 acgataaaat acaagaaccg gttggcggcg ctaatcgaaa ctatgatctt gcggcacaat
    21901 atcttaaaga agctgtaatg cataattttg ccgaattgga aaaactttct gttgagcaat
    21961 tacttgatca gcgtatggaa aaatattcca aaatgggtcg ctggggcgag atgagtgcct
    22021 aaaattatgt tgatcatttg aaaatcacac gaactactgt ccgcgttcgc tacgcggaaa
    22081 ccgatagcat gcgcgtcgct tatcatggca gttatttgcc atggatggaa gtcgggcgta
    22141 ctgaactcat ccgtgaactt ggctactctt acaaacgatt agaatccgaa ggtattttta
    22201 tgcccgtgat tcgtatcgaa gtggattatt tgcgttcggt acattatgac gatacggtac
    22261 atattgatgc ctggattgcg tcgcaagaag gtattcgcac aaccatagct tacatctgtt
    22321 atcgtgacag cgaacctgtt gcccgcggtt ttaccaccca cgcatttaca gatcgtcaaa
    22381 acaaacctgt acgtccgcca aaggagttta tcgcatgctt aaaaaagggt atatgagaat
    22441 cattctcggc tttttttgtt ttgttttatc gctacaggtt tcgctgcatt cacaaaatat
    22501 tgacgcacga ttattcaagg ccatcaataa acatcattcc cccggctggg atcgttttta
    22561 tgaatttcaa agccgatccg tcaaacccgt ttatatagcc actccgctca gttttgttgc
    22621 aaccgggctt ttgcgaaaag acacgtacat gcgtgatgcg ggtattctga tgacgacatc
    22681 cgccgtggct aatctggcta taacctacag catgaaagcg gcgataaacc gtgaacgtcc
    22741 atacaaagcg cttaacaatg tgcatacggt cggaagcacc gaacgttcgg cttcttttcc
    22801 atcagggcac accagttcgg cgttcaatat ggcaacgtct ctttctttag cttatcccaa
    22861 gtggtacgtg attgttcctt cgtatgcgtg ggcgggggtc gtcggctaca gccgcatcaa
    22921 gatgggaatg cattatccgg gtgatgtatt agccggcgct cttatcggcg caggcacttc
    22981 gtatctcaca ttcaaattac aaaagccaat tctcaatacg atccataagt tgttttgaaa
    23041 taactcaaga tggcatcgta gtcttcccgc gtatcaatgt ccaacacaac cccttgatca
    23101 tcactgtcta aataaaaaac ctcgttttga tgattttgaa tcacttcgta aagcggcatt
    23161 tctaaaccgg tttccaagca ttcctccaca attttacgat ccactaaaac aggatgccct
    23221 ttttttccct gataccgggg tattacaatg cgatgcgtta aaccatattg catcatggtt
    23281 ttgatcgttt gtcgtgaaaa caaaggtacg tcgccgggaa ggagcaaaat ggattccgaa
    23341 ctttgatcta tttgttgaac tgaaaaacaa atcgttgcac tcatcccttc gctgtgtcga
    23401 gggtttaaaa aaatccggac aggctctctg attaaggctt gctttatcgt atcgtgataa
    23461 gcccccgtaa taacagcaat acgatcaaca ccggcggcac acaacgtacg gatagcatat
    23521 atcattgccg gtacatcgcc gatgggtaat agtaatttat tatcaccata ccgtgatgcc
    23581 tgaccggctg caaaaataat cgcttcggtt tttttcattt gatttataaa ctgccaagcg
    23641 tgcttttcaa atttgaaaaa cgttcttcca aattttcatc atccatcaaa agtgaaattc
    23701 ccgctgctcc atgagcgcct ttttgtagcc aatgtgagat attatttgtt tgtacgccgc
    23761 cgagaacgaa tatgggaatt ttgacctctt cgcgaattat ttcaaattct ttttcgctta
    23821 gcaccgcatg gcctttcggc ttggatcgcg tttcaccgat ggggccgatc acgataaaat
    23881 cggcgccttc attttccgcg tgaatggctt cgtgcagatc gtgtgtagat acaccgatca
    23941 gtttatccgg gccgaccagt tttctaacat tatatgtagg catgctttga gtcgaaagat
    24001 gaacgccgtc caaatcaagc gccatagcga tatctactct gtcattgata aagatacgaa
    24061 cactgcgcgt cgcggatatt gttcgtatct tgtccgtaag ttcgtacaat gcgcgaggcg
    24121 ataagtcttt ttcacgaatc tgaagcgccc tcaaccctgc tttgacggca cgatccagat
    24181 gcgaaagaaa aagactttcg atcatacgtt tacgatcggt aataccgtaa aatcgaaatg
    24241 gaatattatt tttcacgaga ccaccgaaat ttgtaaacaa aaaaaacacc tatcagcccc
    24301 ggtaaaagca cgttgcttgc aaaaatcatt acggacgtat tgaatgccga agcggcatcg
    24361 actccgaatc tgccgagaaa aaatattaaa gcaccttccc gaaccccgat atccataaga
    24421 gtgatgggca ataatgcttt tacgaacatc gccgcggccg ccgctgataa cgcttcccaa
    24481 aaggaaatcg aatgaaatgc attggcaaaa cagtagattt gtataaaaac aatccattga
    24541 aataataagg catagtgtaa actcggccaa aaatgatggc gtttaaattc gtccgccgct
    24601 gatagcacat ctgtgatatg ttgattccac gggagaaaac gtacccatga ttttagtttt
    24661 tcgatcaacc atcgaatgct tgatggccga aaaatcataa cagccaatgc gacgacaacc
    24721 caaaaaacaa atactaaaat gatcaactgc gtcggacgat taaaatcaaa aacaaattct
    24781 gtaagataca ggagcgatac ggctccgaaa acggcaagcg cgatttgaga taatatttta
    24841 tcaataacac caaaaccaat aatgagtttt ctttgcggcg aatcaataaa aacaccttta
    24901 cccatttctc cgatacgtcc gggcgttatc agaccgatcg aaaatcctgc aaacaacgag
    24961 cgccaaacac tacgccgaga aacatccgga tctatcatac gcacaaggta ttgccatttt
    25021 aaccactgaa taagcaaatt gggcagagtc aatgcagcgc caatccaaaa ataatcccat
    25081 gcgatcgaag aaaaatctgc gcgaatcatc gccggatcaa ctttgaaaat aaggtatccg
    25141 acgaacgcta tcgtaaagat tattttgata tagaatgata attttttact caacataata
    25201 tttcgtttct atcgcaacca tcctgccgga tcaaccgctt cccgatcttt atagatttca
    25261 aagtgaagaa cggctttccc ctcgtcggat tcgtccagat tcgctatggt ttgcccggcg
    25321 gccacatgct gatctaccga cacaaaaatc tccgaaagat gcgcgtaaac cgtataaaat
    25381 ccttcgccat gatctataat aacggtattc ccgaggcggc gtaaccatga aatcaacgac
    25441 acttttcctt tagccacgct atgcacttcc gctccttcgc gggactgaat gtcaacaccg
    25501 gtattacgcg tagtggttcc caatgccgca ttatattgat ttccaaaact taccacgatt
    25561 ttaccgggaa caggataggg caatcgtccc ttatattgag caaaatttga tttttcggta
    25621 taaaccggtt ctttgtattt aacgctgccc gccgttttgg ttttaccgga tttagccgca
    25681 gcggccgcct cttcggctcg tttttttgct aaagcttctt cttcggcttt tttcttctcc
    25741 aatgcagcga taatcgtttg caaattggcc atgtcgtctt tgcgttgttc gatgagcgag
    25801 gccaaaagac gtttatcttt tcgaatcttg ctcagtgtgt tttttcggcg agccaactgc
    25861 tggtccagat tagccttttc gcgttttttt tctgaaataa gaaattgctg attaagcaac
    25921 tctacttcga tcaacttctt ttgtgtttcg attttttctt ttttggtgcg aagactttgg
    25981 atgtcccgtt gatcttgttc ggcaataagt ttgagatatt tcatccgtgc aaaagcttgc
    26041 gtccacgaat cggatgtaag aatgtaagcc aagggatctt catcgcgatg tttatacgtt
    26101 tgtaaaagcc gttgcgacgt gaggcctttg agattttgca gttccgtgcg ggtttgcgaa
    26161 agtattgagc gcaattcggc aatacgctcc tccgtcaatc gacgtccttg ttccaattcg
    26221 cccaataact gatgcagcaa ccctattttt tcattgatat cattcaggtt ttctatttct
    26281 gttttttctt tttgtgcctg agacgtcaat cggttttcat attgagcaat ctcatctttt
    26341 atttttttca tgttgttgcg gcttttatca atctgcttct gaagattttg agcctgcaga
    26401 gaaatcaatg aaaaaaacaa aaatagtgcg acgcgattaa gcatagacat tataatccga
    26461 aacgtggttt tcatcgagcg tgggattatc ctttgggtat gatcgatttt tttcaacggc
    26521 atagtttttt aatccgtcaa aatcctcgga cgtaaaacca gggtggcgca gagggtagac
    26581 cgatttattg gttaatagcg gctcattgac cggaaagtta tacttttggc gcgccatttc
    26641 ccaatccgcc gtccccggaa tcggtgaaaa tgatgaaaga cttaccttag cacctaaact
    26701 gtgtatatag tctattcccg cttgcacttc atccggagat tgatccggta accccatgat
    26761 caaataaacg acaatatcgt gacgaagaaa cccggcttgt tccaaatatt ctaaagccga
    26821 ttctaaatct ttgtcgctga ctttacgtaa aactttttgc ctttccgggt ttttggtttc
    26881 ataactgagg cgaatggttt taaatccact ttgaaccaac aacttagcta actctgcgtc
    26941 gatcagtcgg gcaaaaagtc cattgggtgt gtgaaagttt acattttttc cgttttctat
    27001 gacaccgcgt aaaatccgtt taatatgttt atcgctattt acaaacagcg catcatcata
    27061 aaacgtaata ttttgtatac cttgattcac gtaatttata atttcatgca gcgccccttc
    27121 cagagaacgc ggtgtaaact tttcagtaaa cgcatgcgta gcgcaaaatg tacagctgta
    27181 tggacaacca cgcgatgtca tcatcgcaac atactcaagt ttagagtaca gatgatacgc
    27241 cggatagggg taatcatcat aacccttata gtttttctta acttccaatc ccgactgcga
    27301 gcagagatat gctaaaacgg ttttttcact ttctcccgta aatacacgat cggcgccgga
    27361 aaattccata gcatagttgg taaacaatgt tgcataaatg ccccccaaaa taacttctgc
    27421 ctgaggtact aattctttgc acattgctat gatctcatgt acacccttgt accaatatgt
    27481 catcccggat gtgactaaga cactgtcgac cgattgttct aaatttaggt cggatatttt
    27541 ttttcgcatc agttccggtg taaaaccgta acgcgcataa tgcctcggaa tatgagaaaa
    27601 caccgtgggc ttttcgacaa tttctctata aaaataacca tcgccgtaca tacgggattt
    27661 ggcactcgcg cgaccctgcc atgccagcat ttcggtatca tgccgatcca acgcatcaat
    27721 caaagaaact ctaaaaccat tttcttccaa aacagcggcg atatagagca accccagcgg
    27781 cttggaccaa agatcatacg ccgcaaagtc gtaaatgtac ggattgatca gtagaatatg
    27841 aggttgattt tttttcatta
//
LOCUS       DAOQNQ010000013        27285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_571_length_27285_cov_6.032024, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000013 DAOQNQ010000000
VERSION     DAOQNQ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 27285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27285
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_571_length_27285_cov_6.032024"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PK754_01780"
     CDS             <1..370
                     /locus_tag="PK754_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08266.1"
                     /translation="GIPENGIHAALRSGMLSRESFAKYGLGSMLKRTFDNNLRKAYLP
                     YILPDADTREQLMKDITLLQLIKRQLRLFDEQPILIRIVRYMFYRFAQNSVERTYRRI
                     YERLRKRTQRRLDEILAMMG"
     gene            471..1700
                     /locus_tag="PK754_01785"
     CDS             471..1700
                     /locus_tag="PK754_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNB08267.1"
                     /translation="MINEKEPRFLLITLIATILFGFWFVTIKDVFSPFILATVIIAFL
                     LPFRDHKTIRVLIALIFTLFMLWLIHELREIFTPFLIAFALAYLFDPVVDGFEKRKFS
                     RTSSIVVIILAILGTLFAIGLLVIPQFAGEVSALAGTFPSYEDFKRNLNPDALSFLQS
                     WGMDTAKIMTMIESETTNKITGLIREFTQSAQSLSDGLSSLITQLINLILIPFITFYF
                     LRDFDQNIARIRSRTPERHRAKFEKLYTRIDGILSVYIRRIFLTAFTLGMLSMIILFV
                     LDIRFATIIGITLGLFYLIPYVGYLLTFAVGAIMGLLNPDPGSAVLKIMIVIACIKII
                     DLGIVSPRLVGEKLGLHPVLMIFSIFVFGKLLGLLGLFIAIPVTAILKVLIMEWYDQN
                     FLHREFIRDDSSSTSKE"
     gene            1703..2479
                     /locus_tag="PK754_01790"
     CDS             1703..2479
                     /locus_tag="PK754_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015209418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB08268.1"
                     /translation="MGYSVKDRVILITGASAGIGEATARTLAEAGGKLILTARRKEKL
                     EKLANELKEKFGTSSYTASLDVRDNAAVENFVRTLPDAWSAIDILVNNAGLGRGLDKL
                     HEGKIADWEEMIDTNVKGLLYVSRAVIPGMVARGRGHIFNLGSIAGHETYPGGNVYCA
                     TKHAARAINNGMRIDLVDTPVRVTTIDPGLVETEFSVVRFRGDAERAKKPYEGIAPLR
                     GEDIADLILFAATRPTHVNINEMIVMPKAQATSTLVHRKV"
     gene            complement(2482..5832)
                     /locus_tag="PK754_01795"
     CDS             complement(2482..5832)
                     /locus_tag="PK754_01795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB08269.1"
                     /translation="MKLTEFLFRYRRSIGPVATLVAIVILELAAGQIQNYRPGSIALQ
                     ALIFFSVLLGGLRSGLVSAVLQFLYRLYYMLDFGHPYNEAPEQFWTIVFWIIVNTFAV
                     LAMAWLMQRWHAADARRIQRISQDYDVLLESSSDAVFIMGSDRRYLSVNQKACVMTGY
                     SREELLTMGPLDLIDPDTAHTLDTTLALMQKEGKYLGEQRFKRKNGALFYVEISAKVL
                     NDGRALGIVRDISDRKRREQQLYESEQRFRALIENSSDVVTLVDQRGTILYTSASIKR
                     LLGYETWEISGRSAFDFIQSDADTVQRIFESVRRRENRRVHLSVPLRHKNGSIRWVEF
                     SAINMFHVPFVQAIVTNYHDITERRFAQEALRQSEYNLSGVIENTTDSIYSVDRNLRV
                     VVLNSAFRVAYESAYGVILQPGMAILEYLPKATADEWRSYYQRALEGERFVIEKLFEF
                     AGISHYYDISFYPLRDAQHIITGVSVFSRDVTERMQSRKMISDSEIRYRQMFENNTAI
                     KWLVEPESGRIVDANPAAAAFYGYTVDQLKSLRVWDINVLPEKEVRERIAQAMEAQQT
                     YVDFQHRLANGEIKEVEVRTGPVVIGERKLLFSIIHDVTERRKVEQALIASENKYRSI
                     FENITEGIYQATEEGNFITINPALVRMLGYKSHDEVMRLNLNRDIYVRAEDRLQFNEN
                     TRAQGIGRYAEVFWRKKDGAIIQVRLHDRAVYDPGGRFLYYEVAVEDITESRRLEQNL
                     IQAQKMESVGRIAGGVAHDMNNMLAVILPTAEMLKNFSTSPEMVKTYAENIAASARRA
                     GEIVKQLLVYARQTPLRITRIHINDLIRETQAMVTHAFPKQVRFDIRLDDRIPAMDVD
                     QTQIQQVIMNLLVNARDAMGEPGGLINVRTHSVEFDGESAELLNLPGAGVYIECIIRD
                     SGVGMDAGTLQNIFEAFYTTKPEGQGTGLGLAVAKSIILNHGGTIMVDSKKGEGAEFR
                     FYLPASRSNESAPVPESEKPLETGKETVLVVDDEKAILNICSRILEELGYRVITAQSG
                     MEAVRLYQQEKPDVVMLDIQMPEMDGKQTHAALCGINPMAKIFFATGYATPDVVRDIR
                     QNLKSTIIEKPFSIDTLSKVIRTLLDQN"
     gene            5949..6632
                     /locus_tag="PK754_01800"
     CDS             5949..6632
                     /locus_tag="PK754_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004752234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HNB08270.1"
                     /translation="MTRAQKLYTILCGIFISALIVAEITGSKLIQHSVSENWVFILTM
                     GTIPFPITFLVTDLINEYYGKPGIRFVTLLGMVLIGFVYVILFIDMRIPAAAISPVSD
                     AQFNAVFGQSSRIILGSVTAYLIGQFIDIQIFHTIRKHTGEKWLWLRATGSTFVSQLI
                     DSFVVLFIAFSNTLSFEQIMTIGATNYIYKFVIAIILTPVIYLTHAMIDRYLGVSESH
                     TMIAQSVEK"
     gene            6653..7120
                     /locus_tag="PK754_01805"
     CDS             6653..7120
                     /locus_tag="PK754_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNB08271.1"
                     /translation="MPEILCRVIDCHVFRQTQEGPRYLVMQRANEVIYAGSWRMVGGK
                     IENGEKAWETALRELSEETGLTAMRLWAVPYINSFYEASKDRVNIIPVFAACVQNDNV
                     TLSREHDAYRWASYDEALTLLPWPAQIEGLRIVHEYITTGKTVAGFVEIPLRA"
     gene            complement(7109..8545)
                     /locus_tag="PK754_01810"
     CDS             complement(7109..8545)
                     /locus_tag="PK754_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB08272.1"
                     /translation="MANNRIRNLVSIRSLPGLYEMRRGDRTSAMVMLLSWLLPIVMII
                     YSWQAFLTGWHALVTVPILIVTNFEALTRLWSPEIMEHAVASLAASGWIIGVFYWYHK
                     KRLITQQRTSASPWRLAGQAFRRNGFAMCFLCILMVLVLMAIFAPWYAPYDPNAQQDI
                     VVTKYIKPMHSVTALRLKRNDIVPPRPSAQNFSEYWTATLQSINQQFRQTEEPLLFVD
                     EWHLQGNEVFYTQGIQKKKLDISNLIGTEPIIFLTQRMYWLGSDRFGRDILSRLIYGT
                     RISLAIGMLAMVMAVALGTLVGTIAGYFGHRIDGILMRGVDIMMAFPNLFLILMIVAL
                     FGNSVLLMIAVLGLTGWMGVSRIVRSQVLVLRNSEFITAARALGYSHARIMRVHIIPN
                     TLAPVVVAATLRLGAIILVEAGLSFLGVGVQPPTASWGNMVSDGRDALLHAWWISTFP
                     GLAIVLTVMSFNIVGDGVRDALDPKLRA"
     gene            complement(8535..9023)
                     /locus_tag="PK754_01815"
     CDS             complement(8535..9023)
                     /locus_tag="PK754_01815"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNB08273.1"
                     /translation="MTQKPLYIFDGYNLIKRIDALRREEIKSLEQGRQALLVHIATFR
                     NMTNHDAVVVFDGKKMSLQAKAGVRILFSNLPNRADEAIKRLVDDQAKARTITVVSSD
                     NEVMWYAQGCGCRVERSEDFFQRLTTLKNKPVSDELSAKNDPSLSEREMDYWRSMFEH
                     GE"
     gene            complement(9020..10165)
                     /locus_tag="PK754_01820"
     CDS             complement(9020..10165)
                     /locus_tag="PK754_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNB08274.1"
                     /translation="MKNKLNQWRALFPSTQHGVYVNHAACSPVSTRVKSAIDELLTVS
                     STTHVDNFPVWMETRESLRGILAQMLHTLPARIGFVKNTSAGLNILADGLSWERGDHI
                     LIAEGEFPSNVYPFLKLQDRGVRVEFVPTPTGRFTTDDFVSRFTAATRLVSVSFVQYH
                     NGFRADLEALGRACHARGILFCVDGIQGVGAVPLDVTKTHVDFLSNGGHKWLMAPGGL
                     GFVYVGEQLIDRLTVQQVGWLSVKHPWNLREYALDFPEDARRFELATENMLGIYGMKA
                     SVSLLMEVGIENIFYHITALIDLLAQGLNEQGWIIRSDLTSTHRSGIISFQSRNEQSI
                     PTKNVFEKLMQSGITVSYRDGAIRVSPHFYNTENEIRQILEKLNAWV"
     gene            complement(10162..11079)
                     /locus_tag="PK754_01825"
     CDS             complement(10162..11079)
                     /locus_tag="PK754_01825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNB08275.1"
                     /translation="MKIVLAGATGFIGNALIKALLEEKHHLIILSRRPDVLRNQWGDT
                     VKAVFWDAVSSGDWFSDINEADAIINLTGASIAGKRWDASYKKIIADSRILSTRILMK
                     ACHVAQQKPKTWINASAVGYYGDVPEGDVDETRHASDDFLGRTCAAWENEVLQAETLG
                     IRVVCLRIGIVLEEGGGALEKMVLPFKLFVGGPLGSGRQWLPWIHRDDLVRAVLFILN
                     EKTIRGPVNGTAPMPVTMKQFSQTLGSVLRRPSWAPVPGFVLKIVVGEFSDSLLAGQK
                     ALPVKLQNAGFRFTFDELHAALRDIFIKK"
     gene            11557..13011
                     /locus_tag="PK754_01830"
     CDS             11557..13011
                     /locus_tag="PK754_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNB08276.1"
                     /translation="MKSYRVFFLLLILFVGIGIGLILSFNIGKPRLSAQNSSTADSFP
                     IVHISQALASELAATSFSGIARLSTPAVVTITSEKKQSSSDEETQFRFHRDMPEQGVG
                     SGVIVSADGYIVTNNHVIDQAERIVVRLADRRSFEATLLGTDPLTDIALIRIDATGLP
                     TITFGNSDMIQVGDWVIAVGSPLSLNSTVTAGIVSATNRQIEIIGDQYGVENFIQTDA
                     VINPGNSGGALLNMRGELIGINTAIASRTGMYQGYGFAVPSNIVRNAVQDLSRYGRVV
                     RGYIGVGIKDVDAIYAKAIGLDKARGVLVESLTEKGAARHSGVEEGDVILQIDDMPLF
                     QRNELQAYVALHKPGESVRLQIWRDGRTVEKKVMLRNPDGNTDIPAEKIQRQNDAHIG
                     SESWGYGLGFEVENLSDQGMRGVMISKSSWRAKAQNIYEGMILTRIQKTDINDVSVFE
                     KAARELKNGEAVIIQLADRRNPANHILTAVEVRR"
     gene            complement(13042..14673)
                     /locus_tag="PK754_01835"
     CDS             complement(13042..14673)
                     /locus_tag="PK754_01835"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNB08277.1"
                     /translation="MENSTSEASKPDSVSQSGSDEPPQSNDSQTKTGSLQDEINRIES
                     RIEFSIRKYSAAEIAIIGFAMAILIGSVLLFLIENRGENVISYVDALFISTSAICVTG
                     LTSVDFSKFTFAGQVITMVLIQVGGFGVVTAFRLLWIGRTGNLSPGTAQTLGSVMDVE
                     GEHRTIFQVLASTAKITLAIEGLGCLLLYLATKDRLADGPITPLWFSVFHTISAFNNA
                     GFGLYSDNLMPFRSDVSVNMVIAGLIVLGGIGYPVLLGIESFFLLMLHKMLNFSEALM
                     EMIAMRRPDKLYLVEPFYNFVDRLYLRSKKLREELAGVASPLQMKIVIWGTVFLLAIG
                     TVLMAAYEWDDPHTLGQYDAGEKWLISFFHSVSTRTAGFNTVDIGLLQIRTLMVFISL
                     MFIGAAPQGTAGGIKIPTFATMLGYIRSSFRGMNRVSLFRYVVSKGSVGNAVKLYVLS
                     STFVLSVILLMTVLETRFTLTQMAFEVVSAFGTVGLSAGITMLMTPASKILIVVTMFV
                     GRVGLLTIGSAIIKPTDSSPNTGTWTDDGMKLQIG"
     gene            complement(14812..15204)
                     /locus_tag="PK754_01840"
     CDS             complement(14812..15204)
                     /locus_tag="PK754_01840"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNB08278.1"
                     /translation="MSFIWKDEYLIGDKEIDDQHKNIFHFLTDLEEGVTNGRSDELVS
                     QAIDFLSAYIRDHFKYEEKCMHERKCPVAEYNEHSHKKFLEAYEKIADKYQHDGDSIQ
                     LLREIQALVKVWLVTHIIRIDSHLKKCS"
     gene            complement(15481..16596)
                     /locus_tag="PK754_01845"
     CDS             complement(15481..16596)
                     /locus_tag="PK754_01845"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNB08279.1"
                     /translation="MNWKRICSEFLEIGVNNDYLQKRMDDFRNMSVVMFILGAPFAAG
                     LWEWDYFRDPVGAEQTFLLRLAFFPIFIGVAMVMYRIKDYRLVGIFSVIAMLVTEVFL
                     IQIFERLESGVIYGIGGFMFYFLVSLIGFLGLPLRLTIPYAIAITAFPHLFAFFGYFG
                     GFQHVNFATLIWPAAVLTILGHIAATHNYRKRYEFEISLELATKTDPMTGAVNRRGFM
                     PLLEQEFFRHRRIGHSLALLMMDIDHFKSINDTYGHPTGDQVIRMLANTCEKFSRQTD
                     TVARLGGEEFAVLMPHTEKNMAVMLAERIRIGVEECSQVSDSGIPFRYTVSIGISECQ
                     PDDKTFDQLIARADTALYRAKTQGRNRVIVSGDFDES"
     gene            complement(16629..17111)
                     /locus_tag="PK754_01850"
     CDS             complement(16629..17111)
                     /locus_tag="PK754_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08280.1"
                     /translation="MKVLNAKFFICYKSLIIFLIVLLTFGALSCSCKGSDVIKIPDDE
                     FRYFLIATVGNDFFIVRTSDSVIQSVADSLLLLPLTDRNMMIVGPIDRGNEDYNYDWN
                     WHFKTNAWHFAETAMELCDGSPEYVEEHLDEWLSTVGTYCPWGSRIKKELFAGDVIQK
                     "
     gene            complement(17213..17920)
                     /locus_tag="PK754_01855"
     CDS             complement(17213..17920)
                     /locus_tag="PK754_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08281.1"
                     /translation="MSESTINKAIRAYGGEACWKKAKRIEAEISVRGLAFTLKQRPFF
                     EHALVQMEIARPVSSIIPIGRDPSIRGRLDSEDVWLEDVSGKVLETRNSIRRYFPFGK
                     HAFRWDDLEMSYFANYAMWNYLTLPSLLMNEEILWSETEQGIVKAVFPTSIPTHSKNQ
                     EFRFDKDTGLLCEHLYTVDIIGRYARATHTILAHNEDTGLIYPSRRKVTPQTPWRTAL
                     PGPILIDIEIHRFRLIC"
     gene            complement(17920..18306)
                     /locus_tag="PK754_01860"
     CDS             complement(17920..18306)
                     /locus_tag="PK754_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08282.1"
                     /translation="MDWLKKEGVPVLLASLWIGVSEFLRNEIFFKTYWTNHYLSLGLK
                     FPSDPINGAIWGVWSVFLAILIYVLTSRFSLFQAWLIAWFGGFVLMWIVIGNMGVLPS
                     GILYFAIPWSLLEVLGGALIIRKLAK"
     gene            complement(18502..18912)
                     /locus_tag="PK754_01865"
     CDS             complement(18502..18912)
                     /locus_tag="PK754_01865"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HNB08283.1"
                     /translation="MDSNGKQLQMFYSKALTVNNETNPAEILNSILADQFVSLGSVES
                     KNKMQLIMQLVFFWKLIPDLKWEIQEIINEGNKYVVRSMASGTPNGDFMGMPTDGTKS
                     FKIMTIDIHTMVGGQIAAVHHLEDWPTAIKQLKA"
     gene            complement(19032..19901)
                     /locus_tag="PK754_01870"
     CDS             complement(19032..19901)
                     /locus_tag="PK754_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNB08284.1"
                     /translation="MKEKIETRRFYRNDSRLEVEVVAIEKLKRIGRVVLSKPHRTDFH
                     HVFLVENACTHYVDFKKIKIQPYSLLFIAKGKVHAFDPSIKLQGRIIIFTEHVFSVSE
                     SDVSFLKNTPLFDSLYEIFTIPLSKVLFAKFSTICEDIREESDSNAMRSEPVLVKNLL
                     QNFLIIAERTLRDQGFREKRKDADWDEVVRFRELLENRFTEHKTVFEYADEMGIGLKK
                     LERLTMRILGKSPKAIVHERLLLEAKRLLAYTNRSVKEIAHHLGFDDPSYFVRYFKRF
                     ANITPLEFRETFK"
     gene            complement(20023..20937)
                     /locus_tag="PK754_01875"
     CDS             complement(20023..20937)
                     /locus_tag="PK754_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020606573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB08285.1"
                     /translation="MSPVFRYVVQTSIGYSGMKNEYLKQAVNGDIQAFQKLFLEFQDT
                     LRSYLYRLVTDRHDVEDISQDTFVKAFDKISTFKGESSFKTWVFQIATNLAYDLLRDK
                     KRWKHDAQDQAKAYASSSTMMRQRFLDAHHYSPHGAYHVREHIDFCFTCIAKTLPIDE
                     QITLILKDIYDFSRKEIGMILGLSEGVIKHRLFDARKKMQTIFDSRCALVNKNGVCYQ
                     CTELADFFNPESDHRKTLQNEELIRRANTPDREKLYELRAKLVSQIDPLRSEGSDLQD
                     IIMQCTRIAIGEMGPASPDKKGRAETQI"
     gene            complement(20968..21771)
                     /locus_tag="PK754_01880"
     CDS             complement(20968..21771)
                     /locus_tag="PK754_01880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB08286.1"
                     /translation="MNLIEGKVAVIFAAGGAIASEVAQAMAMEGAHVFLSGRRLAAVQ
                     EIADRILSSGGKAMAHQVDAESENEIIAYIELIANLTGKIDIVFNGIGARPVDLGYGT
                     PSADLPFSLFMKTLQINAGSQFLTSRTAAKIMRRLGSKGTILTLSASLSRLKIPCMAG
                     ITAACSAVEGMSRVLAAEFGSSGIKVICINAAGMPTTRTIRETTALIERTTGVIPIEV
                     DKKTSSNALLGYGPCPKDIGNLSVFLATPAGSIFNSHVIDADFGAANVI"
     gene            complement(22091..23077)
                     /locus_tag="PK754_01885"
     CDS             complement(22091..23077)
                     /locus_tag="PK754_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08287.1"
                     /translation="MKKYWNASRWLIVMSMVNFLLVIGCSEKDSSNLTVDEEEIQNQI
                     IEDADEIIGVDILGESDATGYSKSYDGEAVVLSKSASPIDSIKGYGRVVRMSRRDIQI
                     TVAEDTMSAEAEITFLMKGNFVIRAENPDGEGLVKYTKPLEHEMKRIVRFVRNTDPEI
                     DRRWLVSEVSAGFGISKNGTMSLSSVNVTIHSAAGVTDFGFTDPLAYFIERGGLPQVS
                     QGDTVTVEVGITNSNADDAPMGWIHRGKNRNAKIAGMRVKHLFNDEGTYGDRVTGDGI
                     YTVRWIVGPTEFAGVHVGALDFVTHSTVYESDADAAPYNSLIVALPYRKVIN"
     gene            23495..23770
                     /locus_tag="PK754_01890"
     CDS             23495..23770
                     /locus_tag="PK754_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNB08288.1"
                     /translation="MKLKPLADRVIVEPMEAEEKTASGLIIPDSAKEKPQVGKVVAAG
                     PGKKKDEPLTVKTGDMVLYGKYSGTEITYENKKYLIMSESDILAVQG"
     gene            23827..25479
                     /gene="groL"
                     /locus_tag="PK754_01895"
     CDS             23827..25479
                     /gene="groL"
                     /locus_tag="PK754_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507618.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNB08289.1"
                     /translation="MAAKEIVFDVDARSKLKRGVDVLADAVKVTLGPQGRNVLIDKKF
                     GAPTVTKDGVTVAKEIELEDPIENMGAQMVREVASKTSDVAGDGTTTATVLAQSIVRE
                     GFKNVTAGANPMELKKGIDSAVKVVVAELAKMTKKVEGKEQIAQVAAISANNDNEIGK
                     LLADAMEKVGKDGVITVEEAKGYETILETVEGMQFDRGFLSPYFITDADSQEAVLEDP
                     FILIYDKKISVMKDLLPILEKTVQMGKGLVIIAEDVENEALATLVVNKLRGTLRVAAV
                     KAPGFGDRRKAMLEDIAILTGGTVISEDAGYKLESAKVEYLGRAKKVIIDKDNTTIVE
                     GVGKKETIKGRIAQIKTQIENTSSDYDKEKLQERLAKLAGGVAVIRIGAASEIEMKEK
                     KARVEDALHATRAAVEEGIVAGGGVALLRAQKALEPGRGGINDTLLKGDTGLGVSIVK
                     RALEEPIRQIVENCGIEASIVVQKVREGNDHFGFNALTEQYEDLLKAGVIDPTKVTRT
                     ALENAASVSGMLLTTEATVYEKKENKPAMPPMPPGGGMGDMY"
     gene            25745..>27285
                     /locus_tag="PK754_01900"
     CDS             25745..>27285
                     /locus_tag="PK754_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020472701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HNB08290.1"
                     /translation="MKRNLWLCLWFALFTLSSAQAQQESRLMRFPATDGQQIVFTYAG
                     DLYTVPVNGGLARKLTSDANGYEMFARFSPNGQWLAFSGQYDGNTEVYVMPSGGGVPK
                     RLTITATLGRDDVADRMGPSTIVMAWKNTSEEILFRSRMRSFNDWNGELFSVNLQGDA
                     PSQMPVPRGGWMSFSPDDKKMAYNRIFREFRTWKRYRGGMADDVWIYDFASKTVENIT
                     NNNASDLYPMWVGNKIYFVSDRDARSRFNLYSYDITSKTTTQLTTFTDFDIKFPSLGK
                     DAIVFENGGYIYRFDLKTEKATKVPISIQEDFASGRNALLQVGKNVTNFEISPDGKRA
                     LLGAHGEIFTVPSKAGITRNLTQTSGTHERTSKWSPDGKWIAFISDASGENEIYIMPQ
                     DGSAKPTQITKNADTYYYTLYWSPDSKKILWADKKLRLRYVDVATKNVTEVDQATFWE
                     FSEYTWSPDSKWIAYTRPEDAGMSRVMLYSLDQKKSTEITDGWYESGSPAFSHDGKYL
                     IFGSNR"
BASE COUNT         7707 a         6581 c         5718 g         7279 t
ORIGIN      
        1 gggcatcccc gaaaacggca tccatgcagc gttgcgcagc ggcatgcttt cacgcgaatc
       61 ctttgccaaa tacggactcg gcagtatgct caagcgtact tttgataata acctgcgcaa
      121 ggcttacttg ccctacattt tgcccgatgc cgatacgcgt gagcagttga tgaaagatat
      181 tacccttctg cagcttatca aacgccagct gcggcttttt gatgaacaac ctatattgat
      241 tcgtatcgta cgatatatgt tttaccgatt tgcacaaaac tccgtcgaac gcacataccg
      301 ccgcatatac gagcgcctgc gcaaacgcac gcaacggcgg ctggatgaaa tattggcaat
      361 gatgggttga tttattttta atagttacaa attacaagaa tgtagctact gcctatctac
      421 tcaatttccc cgtgcttttg tcccctgccc tgattagatt cattgcaacc atgattaatg
      481 aaaaagaacc ccgtttttta ctcatcacat tgattgctac cattcttttt ggcttttggt
      541 ttgtcaccat aaaagatgtg ttttcaccgt ttatattagc gacggtcatc atcgcttttt
      601 tacttccgtt tcgcgatcat aaaacgattc gcgtgctcat cgccttgatt ttcacgctgt
      661 ttatgttatg gctgatacat gagttacgtg aaattttcac gccttttctg atcgcttttg
      721 cattagcgta tctctttgac ccggtagtgg acggtttcga aaaacgcaaa ttctcccgca
      781 cgtcttccat tgtggttatc atattggcca tactcggaac attatttgcc atcggacttt
      841 tagtcattcc gcaatttgca ggcgaagtct ccgcgttggc cggcactttc ccttcgtacg
      901 aagattttaa acgtaatctg aatccggacg ccttgagttt tttgcaatcc tggggtatgg
      961 ataccgcaaa aatcatgacc atgatcgaat cggaaacgac gaataaaatc accggcctta
     1021 ttcgtgaatt tacccaaagc gcgcaatcgt tgtccgacgg gctgagttca ctgatcacgc
     1081 aactcatcaa tctgattctg attcctttca ttacatttta ttttttacgg gattttgacc
     1141 agaatatcgc gcgtatccga tcacgtacac cggagcgaca ccgagcaaaa tttgaaaagc
     1201 tttatacccg tattgacgga attctcagcg tttatattcg ccggattttt ctgacggcat
     1261 ttacactcgg tatgctttcc atgatcattc tttttgtttt ggatatccgt tttgccacga
     1321 tcatcgggat cacactgggt ttattttacc tcattccata cgtgggttac cttctgacat
     1381 ttgctgttgg cgccatcatg gggttgctca atcccgatcc cggcagcgcg gttctcaaaa
     1441 tcatgatcgt gatcgcctgt atcaaaatca tagatctggg tatcgtctca ccgcgccttg
     1501 tcggtgaaaa attgggattg cacccggtgc tgatgatttt ttcgattttt gtattcggta
     1561 aattgctcgg actgctcggg ctttttattg ccattccggt gacggccatt ctgaaagtac
     1621 tgatcatgga atggtacgat caaaattttc tgcatcgcga atttatccgt gatgattcat
     1681 cgtcaacatc caaggagtaa atatgggcta ttccgtgaaa gatcgtgtta ttcttatcac
     1741 cggcgccagc gcgggtatcg gtgaagcgac cgcccgcaca ttggccgaag ccggaggaaa
     1801 attgattctc accgcccgtc gtaaagaaaa gcttgaaaaa ctggccaacg agctgaaaga
     1861 aaaattcgga acgtcgtcgt acaccgcttc tttggatgtt cgtgataacg cggccgtaga
     1921 aaattttgta cgcactttac ctgacgcatg gtcggccata gatattttag tcaataacgc
     1981 cggactcggg cgcggtttgg acaaactgca tgaaggaaaa atcgccgact gggaagagat
     2041 gattgatacc aatgtcaaag gtttgctcta tgtcagtcgc gctgttatcc cgggcatggt
     2101 cgctcgcggc agaggccata ttttcaattt gggttccatc gcgggtcatg aaacctatcc
     2161 cggcggcaat gtgtattgtg cgaccaaaca cgccgcacgt gcaatcaata acggaatgcg
     2221 gattgatctc gtagatacgc cggtgcgtgt aacgacgatt gaccccggtc tagtcgaaac
     2281 ggaatttagc gtagtacgtt ttcgcggtga tgccgaacgc gccaagaaac cgtatgaagg
     2341 catcgcgccg cttagaggag aggatatagc cgacttgatt ttatttgcag cgactcgccc
     2401 gacgcatgtc aatatcaacg aaatgatcgt gatgcccaaa gcgcaagcta catccacttt
     2461 ggtacaccgc aaagtataac attagttttg atcgagcaga gtacgaatca ctttggataa
     2521 ggtatcaatc gaaaagggct tttcgataat ggtggacttc agattctgac gaatatcccg
     2581 caccacatcc ggcgttgcgt atccggtagc aaaaaatatt tttgccatcg gattgatccc
     2641 gcacagagcc gcatgtgttt gtttgccgtc catctccggc atctgtatat cgagcatgac
     2701 gacgtcgggt ttttcctgct gatacaaacg caccgcttcc attccgcttt gtgccgtaat
     2761 tacacgatac cccagttctt ccagtatgcg cgagcatatg ttcagaatgg ctttttcatc
     2821 atccacgaca agcaccgttt ctttacccgt ttccaaaggt ttttcactct ccggcacggg
     2881 tgctgactcg ttacttcggg atgcaggaag ataaaatcta aattcggcgc cttcgccttt
     2941 tttggaatct accatgatgg ttccgccatg attgaggata atactttttg caacagccaa
     3001 acccaggccg gtgccttggc cttccggttt ggtagtataa aatgcttcga aaatattttg
     3061 caatgtccct gcatccatac ctacgcccga atcgcggata atgcattcga tatagacgcc
     3121 ggcgcccggc aggttgagca gctcggctga ttctccgtca aactctaccg aatgcgtacg
     3181 tacattgatc aggccgcctg gctcgcccat cgcgtcccgc gcattgacaa gaagattcat
     3241 gatgacttgt tggatttgcg tttgatccac atccatcgca ggaatacggt catccagacg
     3301 tatatcaaaa cgcacttgtt tgggaaatgc gtgcgtcacc atcgcttgcg tttcacggat
     3361 cagatcattg atatgaatcc gcgtaatgcg tagcggggtt tgccgtgcat agactagcaa
     3421 ttgtttgact atttcacccg ctcgccgcgc cgatgcggca atattttcgg catacgtttt
     3481 taccatttcg ggtgacgttg aaaaattttt gagcatctcg gctgtcggta aaatcacggc
     3541 gagcatatta ttcatatcgt gcgctacacc gccggctata cggcccaccg attccatctt
     3601 ttgcgcttga ataagatttt gttcgagccg ccgcgattcg gtaatatctt caacagctac
     3661 ttcataatag aggaaacgtc ctccgggatc atacaccgca cgatcgtgca aacgcacctg
     3721 tataatggcg ccgtcttttt ttctccagaa tacttccgca taccgtccaa tgccctgggc
     3781 tcgcgtgttt tcgttgaatt gcaaacgatc ttcagcccgc acataaatat cacgattgag
     3841 attgagccgc atgacttcgt catgggactt ataacccagc attcgcacta atgccggatt
     3901 gatcgtgata aaatttcctt cttccgtggc ctgataaata ccttcggtta tgttttcaaa
     3961 aatagatcgg tatttatttt ctgacgcaat caacgcctgt tccactttgc gccgctccgt
     4021 gacatcgtga atgatcgaaa aaagtaattt gcgttcgccg atcaccaccg gacccgttcg
     4081 tacttcgacc tcttttattt ctccgttggc cagacgatgc tgaaaatcaa cataggtttg
     4141 ctgcgcttcc atggcctgcg ctatgcgctc tcttacttct ttttcaggca acacattgat
     4201 atcccaaacc cttaggcttt ttaactgatc gaccgtatac ccgtaaaatg cggcagctgc
     4261 aggattagca tccacgatac gcccgctttc cggttccaca agccatttga tggccgtatt
     4321 gttttcaaac atttgacgat accgtatttc actatcggaa atcatttttc gagattgcat
     4381 acgttccgtt acatcgcggg aaaatacgct gacgccggtg attatatgtt gcgcatcgcg
     4441 gagcggataa aaactgatat cgtaataatg gcttatccca gcaaattcaa acagtttttc
     4501 gatgacaaac cgttctccct ccagagcacg ctgataataa cttctccatt cgtcggctgt
     4561 cgcctttggc aaatattcca aaatggccat gccgggttgt aatataacgc cgtaggctga
     4621 ttcatacgcc acgcgaaagg ccgaattgag aaccactacg cgcagattcc gatcgacgga
     4681 ataaatcgaa tccgtcgtat tttcgatgac ccccgacaga ttgtattcgc tttggcgcaa
     4741 tgcttcttgt gcgaaacgcc gttccgtaat gtcgtgatag ttggtaacga tagcctggac
     4801 aaacggtacg tgaaacatat tgatcgccga aaactccacc caacgaatac tgccgttttt
     4861 gtgccgaagc gggaccgata gatgtacgcg ccgattttct cggcggcgaa ccgactcaaa
     4921 aatacgctga accgtatccg cgtcactctg aataaaatca aatgcgctgc gccccgaaat
     4981 ttcccatgtt tcgtagccca gtaaccgttt gatcgaagcg ctggtgtaga gtatggtgcc
     5041 gcgctgatct accaatgtga caacatcgct gctgttttcg atcaaagcgc gaaaacgttg
     5101 ttcgctttca tagagttgtt gctcacgccg cttgcgatcg gaaatgtcgc gtacgatacc
     5161 gagcgctctt ccgtcattca atacttttgc ggaaatctcc acataaaaaa gggcgccgtt
     5221 ttttctctta aatcgttgct cgcccagata ctttccctct ttttgcatca aggccagtgt
     5281 tgtatccaga gtatgcgccg tatcgggatc aatcagatcc aatggcccca ttgtaagaag
     5341 ttcttcacgg gaataaccgg tcatcacaca cgctttttga ttaacggaga gatagcgccg
     5401 atccgatccc atgataaata cggcatcgga tgaagactcc agcaacacat cataatcctg
     5461 actaatacgc tgaatgcgcc gtgcatccgc cgcatgccaa cgctgcatga gccaagccat
     5521 cgccaatacc gcaaacgtat tgacgataat ccaaaaaact atggtccaaa actgttccgg
     5581 agcttcattg tacggatggc caaaatccag catataataa aggcggtata aaaactgaag
     5641 tacggcgctc actaaacccg aacgcagtcc tccgagcagt acggaaaaaa atatcaacgc
     5701 ctgaagagca atcgaaccgg gacgataatt ttgtatctga ccggcggcca attctaaaat
     5761 cacaatagcc accaatgtgg cgaccggacc gatcgaacga cggtaacgaa aaagaaattc
     5821 tgtcaacttc atgagaaaca agaaaggatt aatataatgg ataacataac caaacgatta
     5881 agattaagca acaccatctt gcaattcgcc cgacgaattc tcatattgaa aaaaaacgga
     5941 agcatagtat gacccgcgca caaaagttat atacgattct ttgcggcatt tttatttcgg
     6001 cattgatcgt cgccgaaatc accggcagta aactcattca gcattccgtc tccgaaaatt
     6061 gggtttttat actcacgatg ggtacgatcc cgtttccgat tacttttctt gtaaccgatt
     6121 tgatcaatga atactacggg aaacccggta ttcgtttcgt cacgctgctt ggcatggtac
     6181 tgatcggctt tgtgtatgtt atcttgttta ttgatatgcg tattcccgcg gcggcgattt
     6241 cacccgtatc cgatgcacag ttcaatgccg tttttggcca gtccagccgc attattttag
     6301 gttcggttac ggcgtatctc atcggccaat ttattgatat tcaaattttt cataccatcc
     6361 gtaaacatac cggtgagaaa tggctttggc tcagagctac gggttccact tttgtatccc
     6421 aactcattga ctcgtttgta gtactattca ttgcattcag caatactttg tcattcgagc
     6481 aaattatgac gatcggagca acgaattata tttacaaatt tgtcatcgcc attatcctga
     6541 cacccgtgat ttatctgacc catgcgatga ttgaccgcta tctgggtgtc tcggaatcac
     6601 acacaatgat cgcacagtcc gtcgaaaaat aaatgaatac ggaattcaac ctatgcctga
     6661 aatactttgc cgcgtgatag actgccacgt ttttcgtcaa acacaagaag ggccgcgtta
     6721 tctggtgatg caacgtgcca atgaagtgat ttacgccggt tcgtggcgta tggtcggtgg
     6781 aaaaatagaa aacggggaaa aagcgtggga aacggcttta cgggaattga gcgaagaaac
     6841 cgggcttacg gctatgcgtc tttgggccgt gccatacatc aatagtttct acgaggcttc
     6901 caaagatcgc gtcaacatca tacctgtttt tgcggcgtgc gtacaaaacg ataacgtaac
     6961 actttcgcgt gaacatgacg cataccgatg ggcatcatat gatgaggctt tgactttatt
     7021 accgtggccg gctcaaatcg agggattgcg tatcgttcat gaatacatca ctaccggaaa
     7081 aacggttgcc ggatttgtag aaatcccttt acgcgcgtag tttgggatcc agagcatcgc
     7141 gtactccgtc gccgacgata ttaaaactca tcacggtcaa cacgatcgct aaaccgggaa
     7201 atgtcgaaat ccaccacgcg tgcagcaagg catcgcgtcc atcactcacc atattacccc
     7261 agcttgccgt aggcggctga acacccacac caagaaaact caatccggct tcgactaaaa
     7321 taatcgcgcc taaacgcagc gtcgccgcga caacgaccgg cgctaaagtg ttgggaataa
     7381 tatgcacacg catgatgcgt gcatgcgaat aacccaatgc acgcgccgcc gtgataaatt
     7441 cactgttacg caataccaac acctgacttc gtacgatacg cgatacgccc atccatccgg
     7501 tcaatcctaa tacggcgatc attagtaaaa ccgaattgcc aaaaagcgca acgatcatga
     7561 ggatgagaaa taaattagga aaagccatca tgatatccac cccgcgcatc agaattccgt
     7621 caatccgatg accaaaataa ccggcgatcg tacccaccaa tgtgccgagc gccacggcca
     7681 tcaccatggc cagcataccg atggccagcg aaatgcgtgt accgtagatc agtcggctca
     7741 gaatgtcacg cccaaagcga tctgagccca accaatacat tctttgtgtt agaaaaatta
     7801 taggctcagt acctattaaa ttcgaaatat ccagtttctt tttttgaatt ccttgcgtat
     7861 aaaaaacttc gttaccctga agatgccatt catcaacaaa cagtaagggt tcttccgttt
     7921 gtcgaaactg ttgattgatg ctttgaagtg tagcggtcca gtactctgaa aagttttgtg
     7981 cggatggccg aggcggcacg atatcatttc ttttaagccg caaggccgta acggagtgca
     8041 tgggttttat gtatttagta acgacgatat cttgttgcgc attgggatca tacggcgcat
     8101 accagggtgc aaatatggcc ataagaacga gtaccatcag aatgcataaa aaacacatgg
     8161 caaagccatt acgccgaaac gcctgtccgg caagcctcca cggtgatgcc gatgttctct
     8221 gttgagttat caagcgtttt ttgtgatacc aataaaatac gccgatgatc catcccgatg
     8281 ccgccagaga agccaccgca tgctccatga tttccggtga ccacagacgc gtaagtgcct
     8341 caaaatttgt aacgatcaat attggtacgg tcaccagtgc atgccaacct gtgagaaagg
     8401 cctgccatga atagataatc atcactattg gtaacaacca cgatagaagc ataaccatag
     8461 ctgaggtacg atcgccacgc cgcatttcgt acaaacccgg caaactccgt atcgaaacga
     8521 gatttcgaat cctattattc gccatgttcg aacatgctcc gccagtaatc catttcacgc
     8581 tctgataacg acggatcgtt tttcgcgctc agttcgtcag aaaccggttt atttttaagc
     8641 gtcgtcaatc gttggaaaaa atcttcggag cgctcgactc ggcaaccgca cccttgagca
     8701 taccacatca cttcgttatc ggaagacacg acggtgatcg tacgcgcttt cgcctgatca
     8761 tccacgaggc gtttgatcgc ttcatcagcg cgatttggaa gattggaaaa tagtatgcgt
     8821 acgccggctt tggcctgtag cgacattttt tttccgtcaa ataccactac cgcatcgtgg
     8881 tttgtcatat ttctaaacgt agcgatatgc accaaaagcg cctgacgccc ttgctctaaa
     8941 ctttttattt cctctcgtcg caaagcgtca atacgtttga tcagattgta tccgtcaaaa
     9001 atataaagcg gtttttgtgt catacccagg cgttcagttt ttcgagaatc tgtcgtattt
     9061 cattttcggt gttataaaaa tgcggcgaaa cgcgaatcgc gccatcgcgg tacgatacgg
     9121 taattcccga ttgcataagc ttttcaaaaa cgttttttgt aggaatgctc tgctcgttgc
     9181 gcgactgaaa cgatataatc cccgagcgat gagtggacgt aagatcgcta cggataatcc
     9241 atccttgttc attcaaacct tgcgctaaaa gatcgatgag cgccgtaata tgataaaaaa
     9301 tattttcaat gccgacttcc atcaaaagcg acaccgacgc tttcatgccg tagataccca
     9361 gcatattttc tgtggccaat tcaaaccggc gagcatcttc cggaaaatcc aaagcatatt
     9421 cacgaagatt ccacggatgc tttaccgaca gccaaccgac ctgttgaacc gtcaagcgat
     9481 caatcagttg ttcgccgaca tatacgaaac ccaatccgcc cggcgccatg agccacttgt
     9541 gaccgccatt ggataaaaaa tccacatggg tttttgttac atccaaaggg acggctccga
     9601 caccctgaat cccgtccacg caaaataata ttccgcgagc atggcatgct ctgcccagcg
     9661 cttccaaatc agcacgaaat ccattatggt attggacaaa actaaccgag accaggcgcg
     9721 tcgccgcggt aaatcgcgaa acaaaatcat cggtcgtaaa acgccctgta ggtgtcggca
     9781 caaattcgac acgcacaccg cgatcttgca attttaaaaa aggatataca ttggaaggaa
     9841 attcaccctc cgcgatcagg atatggtcac cgcgttccca cgacaaacca tcagccagaa
     9901 tgtttaatcc cgccgatgtg tttttgacaa agccgatacg cgctggtagg gtgtgcagca
     9961 tttgtgctaa gataccgcgc agactttcgc gcgtttccat ccagacggga aaattatcaa
    10021 cgtgcgtcgt agaagaaacc gtcaaaagtt cgtcaatggc cgatttcacg cgtgtagata
    10081 ccggcgaaca ggctgcatga ttgacataca ccccatgttg agtggacgga aaaagcgccc
    10141 gccattggtt gagtttattt ttcatttttt tataaaaata tcccttagtg ccgcatgcag
    10201 ttcatcaaac gtaaaccgaa aaccggcatt ttgtaatttt accggaagag ctttctgacc
    10261 ggctaataag gaatcggaaa attctccgac aactattttt aaaacaaaac cgggaacagg
    10321 cgcccaactg ggtcggcgca gcactgaacc gagtgtttga gaaaattgtt tcattgtcac
    10381 aggcataggg gctgtcccat taaccggacc gcggatggtt ttttcattta aaataaaaag
    10441 caccgctcgc acaagatcgt cacggtgaat ccacggcaac cattgtcggc cggagccaag
    10501 cgggccgcct acaaaaagtt taaacggtaa aaccattttt tctaatgcac cgccaccttc
    10561 ttcaagcacg atgccgatgc gcagacacac cacgcgtatt cccaatgttt ccgcttgtaa
    10621 gacttcgttt tcccatgcag cacacgtgcg acctaaaaaa tcatcggatg cgtggcgggt
    10681 ttcgtccaca tcgccttccg gaacatcgcc gtaataaccc accgcgctcg cattgatcca
    10741 ggttttgggt ttttgttgcg ctacgtggca cgctttcatc aatatgcgtg ttgataatat
    10801 ccgactatcg gctattattt ttttataaga tgcgtcccac cgttttccgg caatactagc
    10861 tccggtcaaa ttaattatgg cgtccgcttc attgatgtcg gaaaaccaat cccccgaaga
    10921 aacagcatcc cagaaaacgg ctttgacagt atcgccccac tgatttcgaa gcacatccgg
    10981 tcggcgtgag agaatgataa ggtgatgttt ttcttctaat aatgccttta tcaaagcatt
    11041 tccgataaat ccggtcgcgc cggccagtac gattttcatt taaaatgcat ctaagattat
    11101 gaatttgaat agctcaatgt attagattca tctcttaatt tgcacatttt taattctaaa
    11161 ggaaaaaaat taattaattt tattcaaaat aattactttt aaaagtcaaa caaactaact
    11221 tattattaat caacgttttg tgaaaaacaa gctccgtatt attatgtatt tttaattttt
    11281 ttttggatta ctgcaatatt tttcttgcgc agtaacctgt gatatattat attgaaagcg
    11341 tgatcgttga tcacattgtt cttgaaaccc ccacggaggt taccccctaa cctctggcta
    11401 cgatagagac ccagggttgt ttccctcatc cctgggtttt ttattttcat ttttttctac
    11461 tctaaaattc ataaattaat ttggtactaa attacttttt tttcgtacca taataatggc
    11521 tcattacccc acaatacatc gtttgcgcag gtgattatga aatcgtatcg cgtatttttt
    11581 ttattactga ttttgttcgt tggtatcgga ataggactga ttcttagttt taatatcgga
    11641 aagccgcgtc tttctgctca aaattcttcg acagccgatt ctttcccgat cgttcatatt
    11701 tcacaagcgt tggcatccga gcttgccgca acttcttttt cagggatcgc ccggctttcg
    11761 acaccggcgg ttgtaactat tacaagcgaa aaaaaacaat cttcatcgga cgaagaaaca
    11821 caattccgct ttcaccgtga tatgcccgag caaggtgtcg gttccggcgt tattgtttcc
    11881 gccgatggct atatcgtaac caacaatcac gtgattgatc aagccgaacg tatcgtagtg
    11941 cgtctagccg atcgccgctc ttttgaagcc acacttttgg gaaccgatcc actgacggac
    12001 atcgccctca ttcgcattga tgcgacaggt ttgcctacca tcacgtttgg taattcggac
    12061 atgatccaag tcggcgactg ggtcatcgct gtcggctcgc cgctcagtct taattcgacc
    12121 gtaaccgcag gtatcgtcag tgcaacgaat cggcaaatcg aaatcatcgg tgatcaatat
    12181 ggcgttgaaa attttattca aaccgacgcc gttatcaatc ccggtaacag tggcggtgcg
    12241 ttgctcaata tgcggggtga attgatcggc attaatacgg ctatagccag ccgaaccggc
    12301 atgtatcagg gatacggctt cgccgtccca tccaatattg tgcgtaatgc cgttcaggac
    12361 ttatcgcggt acgggcgcgt cgtgcgcggc tatattggtg tcggcatcaa agatgtggat
    12421 gctatatatg ccaaagccat cgggctagat aaagcccgcg gtgtattggt agagtcgctt
    12481 acggaaaaag gcgccgcacg gcacagcggc gtggaagaag gcgacgtgat tttgcaaatt
    12541 gacgatatgc cgctttttca gcgcaacgaa ctgcaagcct acgtcgcact ccacaagcct
    12601 ggcgagagtg tgcgattaca gatatggcgc gacgggcgaa ccgtcgaaaa gaaagtcatg
    12661 ctgcgcaatc ccgacggcaa taccgatata ccagcagaaa aaatacaacg gcaaaacgat
    12721 gcacatatcg gttcagaatc atggggttac gggcttggat ttgaagtgga gaacttaagc
    12781 gatcaaggga tgcgcggtgt gatgatttcc aaaagctcat ggcgtgccaa ggctcaaaat
    12841 atttacgaag gcatgatcct gacccgcatc caaaaaacgg atatcaatga cgtttccgtt
    12901 tttgaaaaag cagcacggga attgaaaaat ggtgaagcgg taattatcca attagccgat
    12961 cgtcgcaacc cggccaatca tattcttacg gccgtagagg tacgccgtta aaaccaatcg
    13021 cttgaacaaa atatcgaacc attatccgat ctgaagtttc ataccgtcgt cggtccatgt
    13081 accggtattg ggtgacgaat ccgtcggttt aatgatcgca ctcccgatcg taagtaatcc
    13141 gacacggcct acaaacatcg tgacaacaat gagtattttg gaagccggcg tcatcaacat
    13201 cgtaattccg gccgaaagtc ctacggtacc aaacgcactt acaacttcaa aagccatttg
    13261 cgtgagtgta aagcgcgttt ccaaaacggt catcaaaagt atcacgctca atacaaaggt
    13321 agaactcaaa acgtaaagct tcacggcatt gcctaccgat cctttgctga ccacatagcg
    13381 gaacaaagag acacgattca taccgcgaaa cgaactgcga atatatccga gcatcgtggc
    13441 aaacgtagga attttaatgc cgccggccgt accctggggt gctgcaccga taaacatcag
    13501 cgaaataaat accatcaaag tacggatttg aagaagtccg atatctacgg tattaaatcc
    13561 ggctgtacgt gtggacaccg agtgaaaaaa tgaaatcaac catttttcgc cggcatcgta
    13621 ctgacccagt gtatgcggat catcccactc gtaagccgcc ataagtaccg taccgatcgc
    13681 cagaagaaaa actgtccccc atattacgat tttcatttgc agagggcttg ccacaccggc
    13741 taattcttca cgtaattttt tggaacgtaa atataaccga tcaacaaaat tataaaatgg
    13801 ttccaccaga tacaacttgt ccgggcgtcg catggcgatc atctccatca atgcttcgct
    13861 gaaattgagc attttgtgaa gcatcagcag gaaaaaactt tcaatgccga gaagaacggg
    13921 ataaccgata ccgcccaata cgatgagtcc ggcaataacc atattcacag atacatccga
    13981 acgaaatggc atcaggttat ccgaatataa gccgaaaccc gcattattga atgccgagat
    14041 cgtatgaaaa accgagaacc ataacggtgt aatggggcca tcggctaaac gatccttcgt
    14101 cgccagataa agcaataagc accccagtcc ttcgatcgcc aaagtgattt tggccgtcga
    14161 agccaaaact tgaaaaatcg tacgatgctc accttcgaca tccatcacgg aacctaatgt
    14221 ttgtgctgtt cccggagaaa ggttacctgt tctaccaatc cacaaaagtc gaaatgccgt
    14281 caccacacca aagccaccga cttgtatcag aaccatcgtt atgacttgac cggcaaaagt
    14341 gaatttgctg aagtcaaccg atgtcagacc ggttacacaa atcgcggacg tcgaaataaa
    14401 cagcgcatcc acgtaggata tcacattttc gccgcgattt tcgatcagaa ataacaaaac
    14461 tgagccgatc aaaatcgcca tggcaaaacc gatgatggca atctcggctg ccgaatactt
    14521 gcggatacta aattcaatcc gactttctat tcggtttatt tcgtcttgta aagaaccggt
    14581 ttttgtttga gagtcgttgg attggggagg ttcatcagat ccggattggg ataccgaatc
    14641 cggtttcgaa gcttcagaag tagagttttc catatatgta ttttggtccg accgtaaaca
    14701 aacgatgtgt ttgtttttgg tggggagcaa tatacaccga gctctattgc aatgtcaata
    14761 aatttgttac gaatacaagt aaggcttcat accaaacaag tggtagtaat tttaagaaca
    14821 tttttttaaa tgtgagtcaa tccgtatgat atgggtgacc aaccagactt ttaccaacgc
    14881 ctggatttcg cggagtaatt gtatcgagtc gccgtcatgt tgatatttat ccgcaatttt
    14941 ttcatacgct tcgagaaatt ttttatgaga atgctcatta tattcggcta ccgggcattt
    15001 gcgttcatgc atacacttct cttcgtactt aaaatgatcg cggatgtacg cgctcagaaa
    15061 atctatagcc tgactgacca gctcgtcact acgcccgttt gtgacgcctt cctccaaatc
    15121 ggtcagaaaa tgaaaaatat ttttatgctg atcgtctatt tctttatcac cgatcaggta
    15181 ttcatccttc caaataaaag acataaattt tttctcacta taggtaggtc aaaagaaccg
    15241 acttaatttg caaggctaca gaactataat caagagataa tgccaaaatt ggtgattttg
    15301 agaaaacaac aaactactta tgatttaatc agattaaagg cattatgccc ttttaagaaa
    15361 agatccgtca tttaagccta taaatgacca tttttgttac ttagcgtata gcgcccaaag
    15421 gccttctagt gagacagatt atgaaaaacg cattaactcg ttaaaccgat tccatccggt
    15481 tcatgattca tcgaaatctc cagaaacgat cactcgatta cgaccttgcg ttttagcccg
    15541 atacaacgct gtatctgccc gtgcaatgag ttgatcaaaa gttttatcgt caggttggca
    15601 ttcagatata ccaatgctaa ctgtataacg aaaaggaata ccggaatcgc tcacttgaga
    15661 acactcctcg acacctatac ggatacgctc tgcaagcata acggccatgt ttttttccgt
    15721 gtgcggcatt aatacagcaa actcctcgcc accaagtcgg gcaaccgtat cggtttgccg
    15781 tgaaaatttt tcacatgtgt ttgctaacat tcgaatcact tgatcgcccg taggatgtcc
    15841 ataagtatcg ttaatggatt taaagtgatc aatatccatc ataagtaaag ctaaagaatg
    15901 cccaatacgt ctgtgccgaa aaaattcctg ttcaagtaat ggcataaatc cccgcctgtt
    15961 tacagctcca gtcataggat ctgtttttgt tgccaactct aaagaaattt caaattcata
    16021 acgtttacgg taattgtgcg ttgcagcaat gtgccctaaa atggttaaaa cagcagctgg
    16081 ccatatcaga gtggcaaaat taacatgttg aaatccgcca aaataaccaa aaaatgcaaa
    16141 gagatgagga aatgcagtga tagcaatggc ataagggatt gtcaatcgta agggtaaccc
    16201 aagaaagccg attaatgaaa ccaaaaaata gaacatgaat cctccaatcc cataaatcac
    16261 gccggattcg agtctttcaa atatctgaat caaaaatacc tcggttacaa gcatagctat
    16321 aactgagaat atcccaacca atcgatagtc ttttatccga tacattacca ttgcgacccc
    16381 gataaaaatc gggaagaacg ccagacgtaa caaaaaagtt tgttcagcgc caacaggatc
    16441 gcgaaagtaa tcccattccc acaaaccggc ggcgaatgga gcgccgagga taaacataac
    16501 gacactcata ttacgaaagt catccatacg tttttgtaaa tagtcattgt ttactccgat
    16561 ttctaaaaat tcggaacaaa tgcgtttcca attcatcgat taatctttcc tatttagagt
    16621 taagcggctt acttttggat cacgtcaccg gcaaataatt cttttttaat gcgcgatccc
    16681 catggacaat acgttcctac tgtgctcaac cactcatcaa gatgttcttc gacatattcg
    16741 ggcgaaccat cgcacagctc catagctgtt tcagcaaaat gccaggcgtt cgtcttaaaa
    16801 tgccaattcc aatcataatt gtagtcttca ttgcctctat caatagggcc aacaatcatc
    16861 atattgcgat ctgttaacgg taatagcaac agactatccg caacactctg aataacagaa
    16921 tccgatgtgc gaacaataaa aaaatcgttc ccaactgtag caatcagaaa atatctaaac
    16981 tcatcatccg gtatcttaat gacatcgctg cctttgcagg aacaggatag agcgccgaaa
    17041 gttagcaaca caatcaaaaa tatgatcaac gatttgtaac aaataaaaaa tttggcattt
    17101 aaaactttca tatgtgtatt tccttaaaaa aacttttgca tgggattcaa atattgaaaa
    17161 tcaacgttta aacatgtacg gccgtaacaa taaatcggat gatgcttgtt actcaacata
    17221 tcaatcggaa acgatgaatt tcaatatcaa ttaaaatagg acccggcaac gccgttctcc
    17281 acggcgtttg cggtgttacc tttctgcgcg aaggatagat gagacctgta tcttcattat
    17341 gcgcaaggat ggtatgcgtt gctcgggcat agcgccctat aatgtccact gtgtacaaat
    17401 gttcacaaag caacccggtg tccttatcaa atcgaaattc ctgatttttg gaatgcgtcg
    17461 gtatactggt tgggaagacc gctttcacga taccctgctc ggtttcgctc caaagaatct
    17521 cctcattcat taataagcta ggcagtgtca aataattcca cattgcataa ttcgcaaagt
    17581 acgacatttc caaatcatcc caccgaaacg catgctttcc aaacgggaaa taccggcgga
    17641 tagaattacg agtttctaat actttaccgg atacgtcttc cagccacaca tcttcactgt
    17701 caagcctacc ccgaatgctc ggatctctac caataggaat aatagatgaa accgggcgag
    17761 caatttccat ttggaccaaa gcgtgttcaa agaagggcct ttgcttgaga gtaaaagcca
    17821 gcccccgcac actgatttca gcttcgatcc gtttagcctt tttccaacac gcctctccgc
    17881 cgtacgcccg gattgcttta tttatagtag attcactcat tatttagcca actttctgat
    17941 tatcaatgcc ccccccaata cttctaaaag gctccatgga atggcaaaat ataatattcc
    18001 cgacggaagc actcccatat taccgataac tatccacatg agaacaaatc ctccgaacca
    18061 agcaattaac caagcctgaa ataatgaaaa ccgggaagtt aatacataaa tcaatatggc
    18121 caagaagacc gaccacacac cccaaatcgc gccattgatc ggatcggatg gaaactttag
    18181 cccgaggctt aggtagtgat ttgtccaata cgttttgaaa aatatttcgt tacgcaaaaa
    18241 ttctgacaca ccaatccata gcgaagccaa aagaaccgga accccttctt tttttagcca
    18301 atccatacag cgcctttcca aattaaacca tactgtgtta ctcttagacg aacgacgacg
    18361 gtaaaacggt cggagaagca acgaggttgc ccttaattgg cggattatca atctataaaa
    18421 aagcttcaaa aggatacaaa aaaacacctt gtcggcaaca aggtgtttta cgagcccagt
    18481 aaaataaaaa tgaacaatca ctcatgcttt cagttgtttt atcgccgtcg gccaatcttc
    18541 caaatgatga accgctgcaa tttgaccgcc taccatcgta tgtatatcaa tagtcattat
    18601 tttgaaagat tttgttccgt ccgtaggcat acccataaaa tcaccattgg gcgtcccgga
    18661 agccatacta cgcacgacgt acttatttcc ctcgttaata atttcctgta tttcccattt
    18721 cagatcagga atcaatttcc aaaaaaaaac aagttgcatg atgagttgca ttttattttt
    18781 cgactcaacc gatccaagcg atacaaactg atcggccaaa atagagttca atatctcggc
    18841 gggattagtc tcgttattga ctgtcaacgc tttgctgtaa aacatttgca attgttttcc
    18901 attactgtcc atggtcgtga aatcctttcg gtgattgatt tactgtttct acgcccttaa
    18961 tatgcgttta cagaaaaggt tgaacaatgg acaaaacgga tttacggtgg tacttttgag
    19021 acaacggcat tttatttgaa agtttcacga aactctagag gcgtaatatt cgcaaacctt
    19081 ttgaaatagc gtacgaagta ggagggatcg tcaaaaccta gatgatgagc gatttcttta
    19141 acggatctat tggtatacgc caagaggcgt ttagcttcca gcaacaggcg ttcatgcact
    19201 atggccttag gggacttccc caaaatacgc atggtcaagc gctccagttt ttttaaacct
    19261 atacccattt cgtccgcata ttcgaatacg gttttatgct ctgtaaatcg gttttccaat
    19321 agctctctga agcgcaccac ctcatcccaa tccgcgtctt ttcttttttc acgaaaccct
    19381 tggtcgcgga gcgtacgttc ggctataata aggaaatttt gtaaaagatt ttttaccaat
    19441 accggctccg aacgcatggc attgctatcg ctttcctcgc gaatatcctc gcatatggtc
    19501 gaaaacttcg caaacaaaac cttacttaaa gggatagtaa atatttcgta taagctatca
    19561 aaaagtggcg tattttttaa aaaagaaaca tccgattcgg aaactgaaaa tacatgctcc
    19621 gtaaaaataa tgatgcgccc ttgcagttta atacttgggt caaaggcatg aactttccct
    19681 ttggcaataa aaagaaggga ataaggttgg atttttattt ttttaaaatc cacataatgt
    19741 gtacatgcgt tttcaacaag aaaaacatgg tggaaatcgg tacggtgcgg tttggataaa
    19801 acaacgcggc cgattctttt tagtttttct attgctacaa cttcaacttc cagtcgagaa
    19861 tcgtttctat aaaaacgacg cgtttcgatt ttctctttca taacaatttt atttatttca
    19921 acaaaatcgc ataaataaat tgtatttttt tgactcgttc aaagcttttt tttcattcaa
    19981 agcctttggt atgaagcaaa accgctatca tcaaccagta aactaaatct gtgtttcagc
    20041 cctccccttt ttatccggcg acgcgggccc catctccccg atcgcaatgc gcgtacactg
    20101 cattataatg tcctgtaaat cagaaccctc cgatcgtaac ggatcaattt gggatacgag
    20161 ttttgcgcga agctcataga gtttttcacg atccggggtg ttcgctcgcc gtatcaattc
    20221 ttcattctgt aaggttttgc gatgatccga ctcggggtta aaaaaatcag ccaactctgt
    20281 acactgatag catacgccgt ttttattcac cagtgcacag cgcgaatcga aaatggtctg
    20341 catttttttc cgggcatcaa agagccgatg tttgatcacc ccttcagata acccgagtat
    20401 cataccgatt tcttttcgtg aaaagtcata tatatctttc agaatcaaag taatttgttc
    20461 atcaatcggt aatgtttttg cgatacaagt aaaacaaaaa tcaatatgtt cgcgcacgtg
    20521 gtaagcgcca tgcggcgaat agtgatgcgc gtcgaggaac cgctggcgca tcatcgtgga
    20581 ggaagaagcg taggctttgg cttgatcttg cgcatcgtgc ttccagcgtt ttttgtcacg
    20641 caacaaatca tacgcaagat tggtcgcaat ttgaaagacc cacgttttaa aagacgactc
    20701 tcctttaaat gttgatattt tatcaaacgc tttgacaaaa gtatcttgcg aaatatcctc
    20761 cacatcgtga cgatctgtga caagccgata tagatacgac cttagcgtat cttgaaattc
    20821 caaaaaaagt ttttgaaacg cctgtatatc tccgttgacg gcttgtttta aatattcatt
    20881 tttcatacct gaataaccta tcgaagtttg tacgacatat ctgaacacag gcgacatgac
    20941 cggcacccac acgacaatat tatactttta tataacattt gcagctccaa aatcagcgtc
    21001 aataacgtgg ctattaaata tactaccggc tggtgtcgcc agaaaaacgg acagatttcc
    21061 gatatctttg gggcaaggcc cgtagcctaa taatgcatta cttgaagttt ttttgtccac
    21121 ttcgatcgga ataacgcccg ttgttcgttc gatcagcgct gtcgtttcac gtatcgtgcg
    21181 tgtggtgggc atacctgccg cgttgatgca aatcaccttg atccccgatg aaccaaattc
    21241 ggccgctagt acacggctca tgccttcgac ggcggagcaa gctgcggtga taccggccat
    21301 acatgggatt ttgaggcgtg acaaacttgc agatagggta agaatagtac ctttagaacc
    21361 aaggcggcgc ataatttttg cggctgtacg ggacgtaaga aattgggaac cggcattaat
    21421 ttgaagagtt ttcatgaaca agctaaacgg caaatcggcg ctgggtgtac cgtatcccaa
    21481 atccactggt cgtgcgccga taccgttaaa tacgatatct atttttccag tcaggttcgc
    21541 gataagctcg atgtaagcga tgatttcatt ttcggattca gcatctacct gatgcgccat
    21601 cgctttacct ccactactaa gaattcgatc tgcaatctcc tgaaccgcag caaggcgtcg
    21661 gcctgaaaga aaaacgtgcg ccccttccat agccatagcc tgggcgactt ccgatgcgat
    21721 agcacctccg gctgcaaata taacggcaac tttaccttcg attaaattca taagcctccg
    21781 gtaatttatg tgttatattt tagacgtagg gcaaccataa aacggtcgga atggctatga
    21841 aaaaataaaa tatacgatta aattatacca cgttagtata aactatttca ctgcactaac
    21901 tgcaaataaa aatacatcaa aatacaaacg gctaaaaatt cagccgtaaa aatggccaac
    21961 atcgtttttt tgtattcttg cttttccata ccgttcattc tttataatta attactcaga
    22021 tttaaaatta acggcggagc ggtttgacgc gctccaccgt ctttcactaa accaaggggg
    22081 aattagttaa ttagttgatc actttacgat acggtaacgc tacgatcagt gagttgtaag
    22141 gtgccgcatc cgcatcggat tcgtacacag tgctgtgagt aacgaaatcc aaagcgccga
    22201 catgtacacc ggcaaattcg gtcggaccta caatccagcg aaccgtataa ataccgtcac
    22261 cggtaacacg atcgccatat gtaccttcat cgttaaaaag atgtttgacg cgcatacctg
    22321 caattttggc attacggttt ttaccgcgat gtatccatcc catcggcgca tcgtcagcat
    22381 tgctattcgt aataccgact tctacggtca ccgtatcgcc ctgactcact tgcggtaaac
    22441 caccgcgttc gatgaagtac gcgagtggat cggtaaatcc gaaatccgtt acaccggctg
    22501 cactgtgtat ggttacattg acgctgctca gactcatcgt tccgtttttg gatataccaa
    22561 atccggccga cacttcgctc acaagccagc ggcgatctat ctccggatcg gtattgcgta
    22621 cgaaacgaac gatacgtttc atttcatgtt cgagcggctt agtatatttg accaatcctt
    22681 cgccgtccgg attttccgca cgaatcacaa aattaccttt cataagaaat gtgatttccg
    22741 cttcggcgct catcgtatct tcggcaaccg ttatctggat atctctacgg ctcatacgta
    22801 caacacgacc ataccctttg atcgaatcaa taggcgacgc ggacttagat aatacgaccg
    22861 cctcaccatc ataggacttg gaataccccg ttgcatccga ttcacccaaa atatctacgc
    22921 cgattatttc atccgcatct tcgattatct gattctgaat ttcctcttca tcaactgtca
    22981 gattggaact atctttttca gaacacccga taaccagtaa aaaattgacc atactcatta
    23041 cgatcaacca tcggcttgca ttccaatact ttttcataca cttcactcct ttatgtttgt
    23101 ggtaaataac ttctaacatt ttatgacccg ctgcataata ttaaaacaat ttatatgcca
    23161 ttttttaaat atttgatatt taatgttata atgattaaaa aaggcaatgg ttgacgaaaa
    23221 aaccgtcgtt tttttaaatt agtcaaaaca aataactttc aatttggtta aaggaaattt
    23281 tgacatagat aaaagacaat tagtcatata ttaagatgac attttagcac tcatgattta
    23341 tgagtgctaa caagtagaat gcaaattaaa taaaaacaaa aaacttgaat ttaggcacaa
    23401 accttgtata tttgcggcca acgaattcaa gctataacca ttatctaata atagtaactt
    23461 cttaactcat acttcataaa caggagattc tctcatgaaa ctgaagccct tagcggatcg
    23521 cgtcatcgtg gaaccgatgg aagccgaaga aaaaacagca tccggtctta tcattccgga
    23581 ttcagccaaa gaaaaaccgc aagtcggtaa agtcgttgcc gcaggtcccg gcaagaaaaa
    23641 agacgaaccc ctcaccgtta aaaccggcga catggtgctt tacggaaaat actccggtac
    23701 tgaaatcacc tatgaaaaca aaaaatatct gatcatgagc gagtcggata tcctcgccgt
    23761 tcaaggttaa tctaatttta gaatactaac cgataacgat aacaatctta caggagatac
    23821 atcaacatgg ctgcaaaaga aatcgtattt gatgttgatg cccgttcaaa actgaagcgt
    23881 ggcgttgacg tattggccga tgcggtaaag gtgaccctcg gtccccaagg ccgtaacgtg
    23941 ctgattgata aaaaatttgg cgcgccgacc gttactaaag acggtgtgac tgttgccaaa
    24001 gaaatcgaac ttgaagatcc catcgaaaac atgggcgctc aaatggtgcg cgaagtcgct
    24061 tccaaaacca gcgatgtcgc cggtgacggt actacgaccg cgaccgtatt agctcaatcc
    24121 atcgtgcgcg aaggtttcaa aaacgtaacc gccggcgcta atccgatgga attgaaaaaa
    24181 ggtattgatt cggctgtcaa agtcgtcgtc gctgaactcg cgaaaatgac caaaaaagtc
    24241 gaaggtaaag aacaaatcgc gcaagtcgct gcgatctccg ctaacaacga caatgaaatc
    24301 ggtaaattgc tcgccgatgc aatggaaaaa gtcggtaaag acggtgtgat caccgtcgaa
    24361 gaagccaaag gttatgagac tattttggaa acggtcgaag gtatgcagtt tgaccgcggc
    24421 ttcttatccc cttatttcat caccgatgcc gattcccaag aagctgttct cgaagatcct
    24481 tttatcctga tctatgacaa aaaaatcagc gtaatgaaag acctgcttcc tattctcgaa
    24541 aaaacagttc agatgggcaa aggtcttgtg atcattgcgg aagatgtgga aaacgaagcg
    24601 ttggctactc tcgtcgtgaa caaactgcgc ggcactttgc gcgtagccgc cgtcaaagcg
    24661 cccggtttcg gcgatcgtcg taaagcgatg ctcgaagata tcgcaatcct caccggcggt
    24721 accgtgatca gcgaagatgc gggttataaa ctcgaaagcg ctaaagtcga atacctcggc
    24781 cgcgctaaaa aagtgatcat tgacaaagac aacaccacga tcgtcgaagg cgtcggcaaa
    24841 aaagaaacca tcaaaggacg tatcgctcag atcaaaacac agatcgaaaa taccagcagc
    24901 gattatgata aagaaaaact tcaggaacgt ttggctaagt tagccggtgg cgttgccgtg
    24961 atccgtatcg gcgcggcttc ggaaatcgaa atgaaggaaa agaaagcccg cgtagaagac
    25021 gcattgcacg ctacccgtgc tgcggtcgaa gaaggtattg tggccggcgg cggcgtggct
    25081 ctgctccgcg ctcaaaaagc cctcgaaccg ggccgcggcg gtatcaatga taccctgctc
    25141 aaaggcgaca ccggtctcgg cgtatccatc gtcaaacgcg ccctcgaaga gccgatccgt
    25201 cagatcgtcg aaaactgcgg tatcgaagct tcgatcgtgg tccaaaaagt ccgcgaaggc
    25261 aacgaccact tcggtttcaa cgcgttgacc gaacagtacg aagatttact caaagccggc
    25321 gttattgacc cgaccaaagt aacccgtacg gctttggaaa atgccgcttc cgtatccggt
    25381 atgctcctta ctaccgaagc gaccgtgtat gagaagaaag aaaacaaacc cgctatgcct
    25441 ccgatgcctc cgggcggcgg tatgggcgat atgtattaat cgtcagctta atacgtctcc
    25501 caagttccgc aggcgaaaac ggccggcgga catcaaatat aaaatccctg ttctgcttca
    25561 tgcggaacgg ggattttttt tggaaagcat gcaacaattt gccgccggta acgttcttat
    25621 ccgatccgta aaaccgtgca ttttatcata cccaatgtca aaacgttttt gtagtattac
    25681 accgtttcca cgtcgttaaa aatgtttttt acatatccat cactatctct gcgaggttaa
    25741 tgttatgaaa cgaaatctct ggttatgctt atggtttgcc ctctttacgc tttcatcggc
    25801 acaagcgcaa caagagtcgc gcttaatgcg tttcccggcg acggatggac agcaaatcgt
    25861 gttcacctat gccggtgatt tatacaccgt ccctgtcaac ggcggattgg cacgcaaact
    25921 tacctccgat gccaatggtt acgaaatgtt cgcacgcttt tctccgaacg gtcaatggct
    25981 ggctttttcc ggccaatacg acggcaacac cgaagtttat gtaatgcctt ccggcggcgg
    26041 tgttcccaaa cgtctgacca tcaccgccac cctcggacgc gatgacgttg cggatcgtat
    26101 gggccccagt acgatcgtga tggcatggaa aaatacatcc gaagaaattc tattccgctc
    26161 ccgcatgcgt tctttcaacg attggaacgg tgaacttttt agcgtcaatc tgcaaggtga
    26221 cgccccatct caaatgcccg taccgcgcgg cggatggatg tccttttctc cggatgataa
    26281 aaaaatggcg tacaaccgca tcttccgcga attccgtacc tggaaacgct atcgcggcgg
    26341 tatggccgat gatgtctgga tttatgactt tgcttccaaa acggtcgaga acattactaa
    26401 taataatgcc tccgatcttt acccgatgtg ggtcggcaat aaaatctact tcgtttccga
    26461 ccgcgacgcc cgctcccgat ttaacttgta cagctatgat attacatcca aaacgacaac
    26521 tcaacttaca acgttcacgg attttgatat caagtttcct tcgctcggca aagacgccat
    26581 cgtattcgaa aacggcggct acatttatcg ttttgatttg aaaaccgaaa aagcaacaaa
    26641 agtaccgatc tcgattcagg aagatttcgc atcgggccgc aatgcgcttc ttcaggtcgg
    26701 caaaaatgta accaattttg aaatttcacc ggatggtaaa cgtgctttat taggtgcgca
    26761 cggcgaaatt tttaccgtac cgtccaaagc cggtatcacg cgtaatctaa cgcagacttc
    26821 cggtacgcac gagcgcacat ccaaatggtc acccgacggc aaatggatcg cgttcatcag
    26881 tgatgcatcc ggcgaaaatg aaatctacat catgccgcag gacggcagcg ccaaaccgac
    26941 acagatcacc aaaaatgcgg acacctatta ctatacgctg tactggtctc cggacagcaa
    27001 aaaaattctc tgggcggata aaaaactccg tttgcgttat gtggatgtag ctaccaaaaa
    27061 tgtaaccgaa gtggatcagg cgacgttttg ggaattttcg gaatatacgt ggtcgcccga
    27121 cagcaaatgg atcgcataca cacgcccgga agatgccggt atgagccgtg ttatgttgta
    27181 ttctctggat caaaaaaaat caacggaaat taccgacggt tggtacgaat ccggttcgcc
    27241 ggctttcagc catgacggca aatatctgat tttcggttcc aatcg
//
LOCUS       DAOQNQ010000014        26357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_613_length_26357_cov_5.829861, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000014 DAOQNQ010000000
VERSION     DAOQNQ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 26357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26357
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_613_length_26357_cov_5.829861"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..5589
                     /locus_tag="PK754_01905"
     CDS             <1..5589
                     /locus_tag="PK754_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB08291.1"
                     /translation="DIMIEGIYDDGFGNTSFVTKLYNNDWATANTPPSDPNGLSAVTV
                     MDTVTMNWNKSTDAETPQDGLTYNIRVGKTSAGVDVMSPLAKVVGSGNAGGFRYVQES
                     GNTSHNNSWRLSGLSDGTYYWSVEAIDQVYRNSKFQSAAQFTIDGAPSAPDSLSVTSG
                     VNSVTLMWGSTNKSDVSKYFIYVSADNEGYTLVDSTTTGIFGTTKTVTKGPSGGILQN
                     GSIYYFRVEAMDHNGYVGPYSNYVTAIPSATPGKWFVVNTDDYGPGSLRSAIDSTNLS
                     TQKDTVVFLIPIGSSITLNSELPAISTDYTVIDADSNDDGKPDVSLSPIGEGGGFFYG
                     LTIQSSNNLIQGLVIGGFGDYYSGGGSLFINGDNNRILGNYIGVSSDGMSTHGNNHGI
                     LINGGSSGNWIGDGTGTGRNIIGGNQQYGIYLNGASTSVNNTKILGNYIGIATDGLTA
                     LPNSGPGISIYNRALNTIIGNGTAEGRNIISGNSSDGILLTDNNGHIEGTSILGNYIG
                     TDVTGSVGVGNNNHGIFFNTLTGEGASIVNNTIGDGTAGGRNVISGNAGSGINFYYYQ
                     VHDNTIKGNYIGITADGSAALGNGMYGVNIEHSNNNIVSSNTISGNAYDGIMINGSYG
                     EANNNVVTGNYVGTNPTGSFAIANGQRGIYLQNGASYNQIGGTAAGEGNIVSGNLNGG
                     IGFGAAYTPTVSNQILGNKIGTDATGMFGIPNKESGIYINGSNHNYNYIGDGTTGGRN
                     IISGNDGDGIYIYGSASDNFILGNYIGVDATGNTGLANDSNGIAIYNSGHILIGDETA
                     GGRNIISGNGKEGIAIYAQSTEIVIGDGGNKALVNDAGGRVAKPELPGANNIDIRGNY
                     IGVGTNGSTSLGNGGAGIEIEAVFEGFYSQYDSITSNLIAYNTNGIELVGAGVSSTYI
                     HNNTIYGNTADGIAIEEGAQNGVTTPAITSISDFVVSGTASVGAMVYVYQDAGNQGRY
                     YLGSSAADGEGSWSYNGLYLPGYNVTATQFLDEQSSAFSAPFATPSGTIHATTTSLSY
                     GNVLAGDSATLTTRIYAIGNPVVMNSGSLVLGTNAQMLATLGTDTLFMTDTITVQVRF
                     KPVNFGFLTDTLRIYYGEGNMFDIRIGGTGTSGTIAASSIGFAMETLNGDSTSVPIQM
                     YTTSGWVQLNSATFGQNNYFTYSIDKTLPYIMKPGDTAGVTVTFKPTTFTNSADTLYF
                     ANNSSDGILELMFSGLPLPGTVATNLASQDFGNVLLGDSLELTTKIYAPSGRVEITSV
                     TLSQGDVLSMTVTPALPFTLNAGDTILVKTKFIPSDFNSFNDTITVLSNASNGTVTVS
                     LSGQSLPGTISSAASAFDFGNVLVGDSAQTSTRYYATGGPVQITGSSLVTSGETSVSF
                     TPSLPYLLHVGDSVVVTAKYKPLTFAAMSADTIHLISNASNSGKLSIPMSGFGQTGTL
                     TFNTTPANFGSIRVADSSTAQIVKVYSSVGQIPLTGYTIGSHFKATTSTVFPITIKSG
                     DTVTLAVKFKPQTTGALLDTIKIGNVRIPLAGTGLENVAPTLTLGVTSVIALKQYLGI
                     YMYSSEALSARSAAFKFNDVSVTAPTFDVVSGVNNLYYAQYKLSAAGSLKIDATGTDI
                     AGNIGTRSKTYTIGSLTKDQTLAVEAEGLRISGAKGAIGESGFMIITKSQTYDGDPVG
                     LAKGTSKSATDFANANIQSWDIISTVALEKSVTLTAVYDETWIHALQTQYANFDERKI
                     GFYREENGSLVYLGGEGRNGNVALQTKSLGKFVIQYNDEHRYLPSEITLAQNYPNPFN
                     PTTTIEFGLPEASHVKLVVYNILGQRVAELFNGQRNEGYHKFVWNGRNAQGATVASGV
                     YLYRLETNKGNVTKKMMFIK"
     gene            5632..6192
                     /locus_tag="PK754_01910"
     CDS             5632..6192
                     /locus_tag="PK754_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08292.1"
                     /translation="MKHFKVWLAGALMIALSAGCSEDPATDPLGDAWQLFINGQYAEA
                     QTSFEALIETQPGQAYAGLGWTAMKLDNIPASVDYFEQAAGDSLVHGYAGWAIASWAN
                     KDFTHIVERAGFVIRKEPAFEFEFYKDITISTMRLMQAYGYYHTGNMSACLARIQELD
                     GGFASTTDPALLLAKLDALSATASGL"
     gene            complement(6278..8095)
                     /locus_tag="PK754_01915"
     CDS             complement(6278..8095)
                     /locus_tag="PK754_01915"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNB08293.1"
                     /translation="MRWFIFFFTTTLFAQTATERIASVSIHGNRIVNDKTLLSLLYHK
                     PGSVFDHEQSRNDAMRILEYYRDRGYLASSIDSIQQKSAEGTDGIRLIFFLSEEKQAK
                     FGSVRISDESDISASQITEVMRWKKGDMFSPGQWTASVNRMLDWFENNGAPFVHIALD
                     SVWMRRDAKSDFIIDILLHIKRRIVKPVGEFIVRGNESTKPLTLTREMRMKLPRGYDH
                     RAIQDGLTRIRRFPFIRSAELTSVTMRPDSLLTVWLDVEEDRANTMDGIIGYVPRDAA
                     RDEKGYFTGLIHVSFYNLFGTARQLSMHWQKKNRLSQDLMVQYTEPWLVGWPVNGRIA
                     VQQQIEDSLYSRSAWEMELQTEITRDWSVLAGVRLQSVEPADPKTAFIFNTPQSDGYA
                     WNGGLTGDMRDEQINPRNGLYYHTVFSYGRTREKNWTLSSDGTDTITYGGEQKIIRSF
                     SQSIRTQTWRMDLEIYREPVHRWVIANGLHFSVFRPGRDVAPLSEQFLFGGLKTLRGY
                     TEGFFSGTRVGWNNLELRYVTGRASRLFVFYDQGYYYRRAYADLERTKINKQDGFPWG
                     YGFGMRFGTRLGQFALDYGLGQNDRPSDGKIHFGFTGTF"
     gene            complement(8525..9136)
                     /locus_tag="PK754_01920"
     CDS             complement(8525..9136)
                     /locus_tag="PK754_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNB08294.1"
                     /translation="MTPDEYYTIAESCTSEIKVKGSKFIGYAAPVQDKDGAEQFLQSI
                     RKNYFDATHHCFAYQLGLTPHVSVRLSDDGEPSGTAGKPILQAITAKNWTDIIVIVVR
                     YFGGTKLGTGGLVRAYGDSAADVLSRCCAEQRLIYENLRIQFPYDEINTIMRCVHHAG
                     GKAGETAYSEQATMDVAIRKNEVLHFCDAITHQTRGRATVIPL"
     gene            complement(9154..10455)
                     /locus_tag="PK754_01925"
     CDS             complement(9154..10455)
                     /locus_tag="PK754_01925"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNB08295.1"
                     /translation="MARDVKAEKLPQTLLLKNVRLLDLIHKSDQVVDILIADGRYVRI
                     GKCDDPADRVIDLSGKVVTHGLMDMHVHLREPGREDKETVWTGANAAMVGGFTAIACM
                     PNTTPPIDTADTVQYVKHKAQGHLVDVHPVGCVTKGREGKELAEIGSLVEAGAVGITD
                     DGDPVYNAEIMRRALEYVKMFNIPVIEHAEDKQLSNRGSMNEGFTSTRLGLRGIPSIS
                     EEVIVARDILLSEYTDAPVHIAHISTGGTVRLVRQAKQRGLKVTCEVCPHHFTLTDEA
                     VENYNTFAKMNPPLRTSADIEEIKLGLQDGTIDIIATDHAPHTIEDKECTMDEASFGI
                     TGLETCVGITLNDLVHKSVISLESALYKLSIAPRKLLHLPLPEFVIGAQANLTILDTE
                     MRWKYDVHQTCSKSINTPYHDYAMKGKAVGVIHNGKIFYAS"
     gene            complement(10457..10852)
                     /locus_tag="PK754_01930"
     CDS             complement(10457..10852)
                     /locus_tag="PK754_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08296.1"
                     /translation="MSQRLLWVMLFGWMMFVGCSHNYYMRSDEPKDETEKEKRRKEIE
                     SAFMYKIKPGYVIQIRTTNGDIHRGVFYSYMDGIVSMRIYDSFRDFELSDITGIKYDV
                     QGGEMKALVFSLILVTMIYIIYEFSQEKH"
     gene            complement(10870..11271)
                     /locus_tag="PK754_01935"
     CDS             complement(10870..11271)
                     /locus_tag="PK754_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HNB08297.1"
                     /translation="MDQDKLAVIGTLYSVIGEEGLRKLVDRFYDIMDTDSSAREIRAM
                     HPTDLTESRDKLFMFLVGRFGGPPVYMEKRGSPALRMRHAPFPINGNARDQWMACMGQ
                     AMREQLHEPGMIDLLNEFFYMVADAMRNQPH"
     gene            complement(11278..13017)
                     /locus_tag="PK754_01940"
     CDS             complement(11278..13017)
                     /locus_tag="PK754_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08298.1"
                     /translation="MKRMMPLLLMILATVVWSGCFETKADYTINPDGSGKVTVEVTTA
                     GMDGMMGNMSGKTPDGSKTARDIIQQATGVDTWKDVEFRVLDDGRNYFKGTAYFKNIS
                     NVKFKNAGAGDSIVVYQDAQKNWVLELATNPSKKSDKAKSGSEKIPTTDAEITKAVKE
                     QKNGYNQMRPMLLAIMGTMKSEMNFRLPGKAQSSNNMTQQADGSYQVSFTGEKFIQVL
                     DKLFADENWLKEQARAGANIKDNPPSGDAVNELLFGQKGPVRAVVSGKTAPQFNYEAE
                     VSAAKAGYDAMIERLGLNQTESSATMESSKPERAVQSYQGGTFDWVKITRVTYSNVED
                     PDYLQFGTDQSYQVTFTGALPGAIVKTERAVVTAAIADNGQNLLHESEWYRETNYVEM
                     SKQKDFLRWTLTLTVPDATAKGIKRIEGTVYAMRSTSTSVVDPGIRKFEKGAEGNQYG
                     AVIEDIQDDEYTHQMVIRVGLPDFKLNKMDILDEKGNPVGISDRYISTYTEESTTMVL
                     NLNTAVPKRGTINIHIHDDVEGVELPFVVENVNFLGQSMSPAVEQKQNKPQEKDLPRD
                     PVKEKTKGKGKKK"
     gene            complement(13063..14265)
                     /locus_tag="PK754_01945"
     CDS             complement(13063..14265)
                     /locus_tag="PK754_01945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNB08299.1"
                     /translation="MHRIRLKSGKEKKIRNAYPWIFRDDIATQFKDRGDIPNGAIVEI
                     ADAEDKFLAVGYYNALSHIVVRVLDRKNRPIDKNFFKQRIQRAFDFRTQLALPSDARR
                     LIHAEGDRLPGLMVDQFGAYLVVQFRSLGMEKFRTEILETLVEVVKPKGIYDRSDMES
                     REEEGLEPFSGVVYGAIPEEIVISENDLLFHVDIHHGHKTGFYLDQRENRAYVRTLVK
                     PNQTGLDLFSYTGGFAVALAQSGVRMTAVDQDAAALEGGRANARRNNVEKRLQFIHAD
                     VFKYMEEQVAVKTSTPDKVKKYDVIIIDPPAIAKRKEGIEKLKWAYWKLLHQAIQLLN
                     PGGHIVLSSCAYHMPVDLMLEAARFASADNGVSMRVVNITYQPPDHPWMLQIPETLYL
                     KTVYFQLM"
     gene            14728..15744
                     /locus_tag="PK754_01950"
     CDS             14728..15744
                     /locus_tag="PK754_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08300.1"
                     /translation="MRKLMTMIALLALCSVQTYAKTWTVDQRPGANFQTIAAAVASSS
                     VLAGDTILVSGSSIAYAGFSFNKKLTIIGPGYFLGENTNLQADTNSVKISSQVTIQSG
                     AQGSIVMGLHFLNSLYVDADNVQIIRNRFQITSTYDYGIYLYAQDSTIIRQNYLTQTA
                     SWYHCITIASGNTQTMIQNNFLEYTGGDSNSSYGALSVQGTISGDISNNVIYGNVTLS
                     AGGFYFNNNILRYGKFNSTGITPHNNIGSSSQFSFWPALGNQSGVNMTSVFVSSGTSD
                     TKWQIATEGPADNNGSGGVDCGMYDNSAGTAYIPSGIPPIPSIYQFDASDLNNVTVKV
                     KSHN"
     gene            15804..19136
                     /locus_tag="PK754_01955"
     CDS             15804..19136
                     /locus_tag="PK754_01955"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HNB08301.1"
                     /translation="MKFFTALIGLWLSATSLFGQSPPDVHSVEYFIDTDPGFGLGTSV
                     APSAATSGSDKTFVFSVSTTGLPSGFHTLYVRSRDNNGGWSQATAKAFYVMPGESGSF
                     ATAVQRVEYFIDTDPGFGNGIAMGSPSSGDATVTGMIDISALPGGFHTLYVRARDGFH
                     KWSVLNSRTFYKSVVYTPPTDPQVTYIEYFVDKDPGYGNGTSVPGFTASSDITHNFLV
                     DIGALSGGFHTLYIRTKDANGNWSIASTKAFYKPHNYVAPSDPQITAMEYFIDTDPGF
                     GAGTALSFTASSDVTVNGVVDITSVSGGFHTLYLRSKDANGNWSVAQAHAFYKAHTVS
                     NTSSNITAIEYNFYKSGSSTPVRTFTAFTPSTDVQTTFLADLSGLNIDSTYDMRVTAI
                     DAAGRRSIAATASTVIIERPNHAPEIKSSVADVTANEDFDSLIVASLDTVFHDSDMTT
                     VGDHLQFNASVNGSAVTSVLRGSQLVIFSSPDIFGSAEVIITATDDSSASVSDTIHVT
                     VNSVNDRPLATYDVAQANAGYTAYVQPLKNDTDIDGDALVIAAIVAMPQHGTATVVSP
                     ADTLISYTPTVSYSGMDSIQYAVSDGLGGLDTAIIYLQINGYINSAPQRNNTIADVTA
                     AEDFGVIALAQLNSVFGDNDIALNNDSLRYSVSVQPAIVALSLRHDTVVVNSIANLFG
                     IAEVIVTATDDSGATASDTLTVTVNSVNDAPFTQNDAVSTTGIQTVVAHPLNNDTDVE
                     SQTLTIAAIITSPQHGSATIPSGDTTIAYTATIGYSGNDSLTYSVSDGNGGLDTAKLV
                     ITIQAPAPLSLSTSVHQNPALAQYADVYVIADTALIGLPDVKMFLGSDSTALTMTVST
                     SRVYRGQFEFASSGAYTIRTRATAATGAQAVNFRSFTATLAKPGVTTNIASTDAQAQL
                     LVKSKAIVSPTYITVATDESMNGKAYQFAPALTLQEPSEITIRFEPSQYEDAGKIFIE
                     SETDGRIVRLQTGVYPEQRIAKAKITSLGKVRIVYDPSFTGSNTVPTNFSLKANYPNP
                     FNPSTTIAYDLAKEGLVTLTVYNVLGQKVKTLVHGHAPAGSYKLLWDATDERGKRVST
                     GVYFYRLETPGFVQTRKMLLMK"
     gene            19145..19729
                     /locus_tag="PK754_01960"
     CDS             19145..19729
                     /locus_tag="PK754_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08302.1"
                     /translation="MKNKIIFCALAIVLLSSCGPDPGTSEDYAATVAEGWTFFETGSY
                     PQAKERFRAAIAIDPAFSEAFTGLAWSQMKTDSLDEAVTVFSFGSATSDATADLWAGW
                     AFTEHALKQYTASNGRITNAIAADANWIFSHTAGLSVSDLRVLTAENHFALGSYAASL
                     SAVQQLNAAFTADVTTTAGQSALAAEIERLKGIN"
     gene            complement(19908..20666)
                     /locus_tag="PK754_01965"
     CDS             complement(19908..20666)
                     /locus_tag="PK754_01965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB08303.1"
                     /translation="MNTFKDNFSKQAEVYAKFRPTYPKELFEFLQGLTAEHKLAWDCG
                     TGNGQSAIKLTDYYEKVYASDPSQEQIKNAIPHDRIVYKVENAENPGAIADNSVDLIT
                     VAQAIHWFDFDKFYTQVKKVLKTNGIIAVWAYGIPTINEELDKIIKNFHDNIVGEFWL
                     PENRLIEKEYLTIPFPFDEIKTPDFFIKRLVTLSETLGHLRSWSATQKYIDKYNENPI
                     EHLSQKLQEHWKDIETKKEITWKLILKLGKIASR"
     gene            complement(20817..24086)
                     /locus_tag="PK754_01970"
     CDS             complement(20817..24086)
                     /locus_tag="PK754_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNB08304.1"
                     /translation="MFLAISRFEIKYWLKNWSFYTYLSVFFLMSLLSMAGASGGFGEG
                     SASAENIANSPMSIYGFANFFNKFLLFVIPAIVGNTIYKDYKSNFKGILHTYPFSKTD
                     YLFAKFTSAFLILCLIAFSVLLGLIFGTKLPTVNPIQVLPFDAVPYLHIYFIYLIPNL
                     LLFSIVVFSIVLLSRNIYTGFISIILIWLFKEAAIRLVGVEGLASLLIDPFGESAVQY
                     FTRYWSLSEQNTLSLPLSPIVLINRFVWLLIGAFIFIATFRWFSFSQNPFSFCFKTSK
                     SESLTKNNFGSIIKINLIKQKFDFSFFQQIKTSWKLSQTDFYFIIKNGAFISIVIAGI
                     IFIVAILLQINPQTDIKILPVTWVVLGFPVFFFSFLIQILTFLYAGILVHRAKNSKIS
                     DLISVTAVPNWVLLFSKLLALVKMQLLLLSLIMAVGIAIQTYSNYYHYEIGHYLFDLL
                     VIHLISFIIWAFLSLFVQSMFGNVYLSLFLLILFVLGVSQLPSLGIENFAFRFNESPN
                     SDFFLKYSDMSGYGHSLSAYFLYKLYWLVFGLFLFCITLLVWQRELTNSIHERLKIAK
                     KRFAGKLAFISIVLMIIFLFFGFFLFQKENAPKNKMLSVKNEDNLLSQFQKKYGKYKH
                     TKQPRITSVFVKLDIFPETNSFIAKGKYTLINKTEYAIDTLLIKKGFDEITTLSFDQQ
                     AALIEEDTIFEFCMYKLDKGVSPNDSINLNFTIKNQKNTLLTQNSNILKNGTYLKSDI
                     FPRLGYFADTEKKIPTNSSSFANHYQSIDADLVNFEAIVSTSPQQIAITSGNLLREWL
                     EDGKRYFHYKMDKPFKFVFGFNSGEFAVLKENYKGVDLRVYYHPKHIYNIQQMMDGLK
                     ASLDYNVLHFGTYQYKQVHIIEFSRSEGSYATTAGNCVPISEIRFINDARNLKEDGID
                     LSFYVAAHELSHQWWGNQVIPANALGATMITESIAEYITAKIYEKKYGIQSALKFLQI
                     QRKRYLLGRANETEQEPPLYLVNPEQSYIAYGKGAIALYTLSEFIGVEKLNTALKEYL
                     DKVKFQPPPYTTSIEMLTYLKKATPDSLQYLITDMFEKNDKEKTLLYFDKILERNNAS
                     H"
     gene            complement(24091..24972)
                     /locus_tag="PK754_01975"
     CDS             complement(24091..24972)
                     /locus_tag="PK754_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004919307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB08305.1"
                     /translation="MTLKINNLSKTYPNGVKALDNLNLEIKQGMFGLLGPNGAGKSSL
                     MRTLATLQPSDEGSVFFEDIDILKDPMELRKILGYLPQDFGVYPKMSAEDLLEYLATL
                     KGIASKQERNALIKQVLEITNLYEVRKKSVDGYSGGMKQRFGIAQLLLNNPKLIIVDE
                     PTAGLDPAERNRFLNVLREIASSNIIIFSTHIVDDIKDLCNDMAIMNGGKILKKIMPS
                     EATKEIESKIWTSNIDREKLEEAEQAFFVLSSKYNQDNSVNIRVYANEQPSSNFASAT
                     PNLEDVYFIALKNNLKN"
     gene            complement(25149..25745)
                     /locus_tag="PK754_01980"
     CDS             complement(25149..25745)
                     /locus_tag="PK754_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobile mystery protein B"
                     /protein_id="HNB08306.1"
                     /translation="MGLEIEYIEGQTPLDEDEKEGLLIKSITTRGELDEFEQLNIEKS
                     VQWTIGKKWKPEYILSEIFVKELHKRMFNEVWSWAGNFRKTNKNIGVDKHQIGFSLKQ
                     LLDDSLYWLKNKTYTDEEFAIRFKHRIVKIRCFPNGNGRHSRLIADIIISQLFGKPIF
                     TWNSTNLNKKGEARSVYLTAIRQADSGDIKPLIDFAKT"
     gene            complement(25736..26200)
                     /locus_tag="PK754_01985"
     CDS             complement(25736..26200)
                     /locus_tag="PK754_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobile mystery protein A"
                     /protein_id="HNB08307.1"
                     /translation="MDISKQRLIIEQADRKLALFRPLSSFSIPPKGWIHTIRIALKMS
                     LRQLGNRLKISPQSVKEIEEREANGSITIKGLREVGAVLDMKLVYGFIPKGQSIEEMI
                     EKRALDIAKDIVMRTSYTMQLEDQENSKIRIEKAIKNRAEEIKTKMPKYLWD"
BASE COUNT         7261 a         6042 c         5524 g         7530 t
ORIGIN      
        1 gatatcatga tcgaaggtat ttacgacgac ggttttggca atacatcctt tgtcacgaaa
       61 ctctataaca acgattgggc aacagccaat acaccaccat ccgatcctaa cggactgagc
      121 gctgtgaccg tcatggatac agtcacgatg aattggaata aatcaacgga tgccgaaaca
      181 cctcaggatg gtttgactta caatatccgc gtaggtaaaa cctccgccgg tgtggacgtc
      241 atgtctccat tggccaaagt cgtaggcagc ggcaacgccg gaggattccg ctatgtacag
      301 gaatccggca acacaagtca taataattca tggcgcttat ccggtctatc cgatggcacc
      361 tactactgga gtgtcgaagc gattgatcaa gtttatcgca attccaaatt tcaatcggcg
      421 gctcaattca cgatcgacgg agctccttct gcgccggaca gtcttagcgt tacttccggt
      481 gtcaattccg ttactttgat gtggggttca acaaacaagt cggatgtaag caaatacttc
      541 atttatgtca gcgcggataa cgaaggctat acacttgttg actctaccac gaccggaatt
      601 tttggtacga ccaaaacggt gaccaaaggt ccttcgggcg gcatacttca aaacggttcc
      661 atctattatt tccgcgtcga agccatggat cacaatggtt atgtaggccc gtattcaaac
      721 tatgtaacag cgataccatc cgctacaccc ggtaagtggt ttgttgtaaa tacagatgac
      781 tacggccccg gatcattacg ttcagctatt gattcgacca atctttcaac acagaaagat
      841 accgttgtgt tcctcatacc catcggttcc agtattacat taaattcaga acttcccgcg
      901 atctcgacag actataccgt gatcgatgcg gacagtaacg acgacggtaa gccggatgta
      961 tcactctcac cgattggcga aggcggcgga tttttctacg gattaactat tcaatcttcc
     1021 aacaacctta tccaaggact cgtaatcggc ggtttcggcg attattattc gggcggagga
     1081 tcactcttta tcaacggaga caataaccgg attcttggta actatatcgg cgtttcatcc
     1141 gacggtatgt caactcatgg taacaaccat ggcattctga tcaatggcgg ctccagcggt
     1201 aattggatag gcgacggcac aggaaccgga agaaatataa ttggaggaaa tcaacaatac
     1261 ggaatttatc tcaacggcgc ttccacgtcc gtgaataata cgaagattct cggcaattat
     1321 atcggtatcg ctacggacgg tttaacggcg cttcccaatt cgggtcccgg tatatccatc
     1381 tataacagag cgcttaatac aataatcggt aacggcacgg ccgaaggtcg caatattatc
     1441 tcaggaaata gcagcgacgg tatattgttg accgacaaca acggacacat tgaaggaact
     1501 tcgatactgg gcaactatat cggtacggat gttaccggca gtgtcggcgt gggcaacaat
     1561 aatcacggta tcttttttaa cactctaacc ggcgaaggcg cctccatcgt aaataacacc
     1621 atcggtgatg gtacggccgg cggacgcaat gttatctccg gaaacgccgg tagcggtatt
     1681 aatttttact actaccaagt acatgataat actatcaaag gaaactatat tggcataacg
     1741 gccgacggct ctgcggctct tggcaacggg atgtacggtg tgaatattga acacagcaat
     1801 aataatatcg tatcctccaa tacaatatca ggaaacgcct atgacggtat catgatcaat
     1861 ggctcgtatg gtgaagctaa caacaatgtg gtaaccggta attacgtagg aaccaaccct
     1921 acaggatcat ttgccatagc taacggtcaa cgcggtatct accttcaaaa cggtgcttct
     1981 tataaccaaa tcggtggtac ggcagccggc gaagggaata tcgtttccgg taacctcaac
     2041 ggtggtatag gatttggtgc ggcttataca cccaccgtat ctaatcagat tcttggcaac
     2101 aagataggaa ctgatgcaac cggcatgttt ggtattccca ataaagaatc cggtatttac
     2161 atcaacggct cgaatcacaa ttataactat atcggcgatg gtactaccgg tggtcgtaat
     2221 attatttccg gaaacgacgg cgatggtatc tatatctacg gcagtgcctc tgataatttt
     2281 attttaggca actatatcgg cgttgatgca accggtaata ccggattagc caatgattcc
     2341 aacggtatcg cgatttacaa ctccggtcac attctgatcg gtgatgaaac ggccggcggt
     2401 cgtaatatta tctccggtaa tggaaaagaa ggtatcgcta tttacgctca aagcaccgag
     2461 attgtcattg gcgacggagg gaacaaagct ctggtcaatg atgcgggagg ccgtgttgct
     2521 aaacccgaat taccaggggc gaataatata gatatccgtg gcaactacat cggtgtcggt
     2581 acaaatggat caacctcact cggcaatggt ggtgccggta ttgaaatcga agctgtcttc
     2641 gaaggttttt attctcaata cgattcgatc acttcaaatc tcatagctta caacaccaat
     2701 ggtatcgaat tagtaggcgc cggtgtttcg tcgacctaca ttcataacaa tacgatttac
     2761 ggaaacacgg cggacggtat cgcaattgaa gaaggcgctc aaaacggagt caccactccg
     2821 gcgattacct ccatatccga tttcgtcgtt agcggtacgg cttcggttgg cgccatggta
     2881 tatgtttacc aggacgccgg caatcaggga cgctactacc tcggttcttc cgcggctgac
     2941 ggcgaaggtt catggtcata caacggactt tacttacccg gttataatgt aacagcaacg
     3001 caatttttag atgaacaatc ttcggcgttc tctgcaccat tcgctacacc gtccggcact
     3061 atacatgcta cgaccacatc gctttcttac ggcaatgtct tagccggtga tagtgccaca
     3121 ctaaccacgc gcatttatgc cataggtaat cctgtggtaa tgaatagcgg ttcgttagta
     3181 ttgggaacga atgcacagat gttagccacg ttaggaaccg acaccctctt tatgacggat
     3241 acgatcacgg tacaagttcg tttcaaaccg gttaactttg gatttcttac cgatacactc
     3301 cgtatttatt acggtgaagg taatatgttt gatattcgta tcggtggaac cggcacgtcc
     3361 ggtacaatag cggccagcag catcggattt gcgatggaga cactcaatgg cgacagcaca
     3421 agcgttccta tccagatgta cacgacaagc ggttgggtgc aattgaactc cgctacattc
     3481 ggtcagaata attatttcac ttattcgatc gacaaaacgt taccttacat aatgaagccc
     3541 ggcgatacag ccggtgtgac ggttacattt aaaccgacaa catttaccaa ttcggccgac
     3601 acgctgtact ttgcaaacaa ttcatccgat ggtatacttg aattgatgtt tagcggttta
     3661 ccgcttccgg gtacggtagc gacgaatctt gcatcccaag attttggtaa tgttctattg
     3721 ggcgatagct tggaattgac gacaaagatt tacgctccgt caggccgggt cgaaatcaca
     3781 tccgtcacac tttcccaagg tgatgtactt tcgatgacgg taacgccggc attacctttt
     3841 acgttgaatg caggggacac catcctcgtg aaaacgaaat ttattccgtc tgattttaac
     3901 tcgtttaacg acaccattac ggttttaagt aacgcctcca acggaaccgt tactgtgtcg
     3961 ttgtccggac aatctttgcc cggaaccatt tcgtctgccg cttcggcgtt cgattttggt
     4021 aatgtgttag taggtgacag cgctcaaacc tcaacccgtt actacgccac aggcggtccc
     4081 gttcagataa ccggttcttc actggtaaca agcggtgaaa caagtgtcag ctttacaccg
     4141 tcattgccct atttgctgca cgtaggagac tctgttgtcg ttaccgccaa atataagccg
     4201 ttaacatttg cggcgatgag cgctgacacg atccacctga tcagtaacgc atcaaacagc
     4261 ggcaaactga gcatcccgat gagcggtttc ggtcaaaccg gtacgttgac ctttaatact
     4321 acaccggcta atttcggttc gattcgcgtt gccgatagca gcacggccca aatcgtcaaa
     4381 gtatacagca gcgtaggtca gatcccgctt acaggatata ccatcggctc tcatttcaaa
     4441 gcgacgactt cgaccgtatt ccctatcacc atcaaatccg gtgatacggt tacgttagct
     4501 gttaaattta agccgcagac caccggcgct cttttagaca ccatcaaaat cggaaacgta
     4561 cgtattccgc ttgcgggcac cggtttagaa aacgtggcgc ctacgcttac gctcggcgtg
     4621 acaagcgtca tcgcactcaa gcagtacctt ggtatttaca tgtatagcag cgaggcgttg
     4681 agcgctagat cggctgcatt taaatttaat gatgtcagtg tcaccgcacc gacatttgat
     4741 gtcgtatccg gtgtaaataa cctgtactat gcgcagtaca aattatctgc ggccggttcc
     4801 ctcaagattg acgctaccgg tacggacatt gccggaaaca tcggtacgcg ttccaaaacg
     4861 tacacgatcg gctcattgac caaagatcaa actttggctg tcgaagcgga aggtttgcgt
     4921 atcagcggcg ctaaaggcgc tatcggtgaa agcggtttta tgattattac caaatcgcaa
     4981 acctatgacg gcgaccctgt cggtttagcg aaaggtacgt ccaagtcagc gaccgatttt
     5041 gcgaatgcca atatccaatc atgggatatc atcagcaccg tagcgcttga aaaatccgtg
     5101 acgctgacgg ccgtctatga cgaaacatgg atccatgcat tacaaacgca atacgccaac
     5161 ttcgacgaac ggaaaatcgg tttctaccgc gaagagaacg gctcattggt ttatctgggc
     5221 ggtgaaggtc gcaacggtaa cgttgcactt caaaccaaat cgcttggtaa gttcgtgatc
     5281 cagtacaatg acgaacatcg ttacttgcct tcagaaatta cgttggcgca gaattatccc
     5341 aatccgttta accccacgac aacgatcgaa ttcggccttc ccgaagcctc gcacgtcaaa
     5401 ttagtcgtgt ataatatcct cggacaacgc gtcgcagagc ttttcaacgg ccagcgcaac
     5461 gaaggatatc ataaattcgt atggaacgga cgcaatgcac aaggcgccac ggtggccagc
     5521 ggcgtgtatc tgtatcgcct cgaaaccaac aaaggtaacg tgactaagaa aatgatgttt
     5581 atcaaataac gtacgatata taaaaaaatt atataacaaa ggatcatgat tatgaaacac
     5641 ttcaaagtat ggctggccgg cgcactgatg atcgcgctta gcgcaggatg cagcgaggat
     5701 ccggcgaccg accctctcgg ggatgcatgg cagcttttta taaacggtca atacgctgaa
     5761 gcacaaacca gttttgaggc ccttatcgaa acgcaacccg gtcaagccta cgccgggctc
     5821 gggtggacgg ccatgaaatt agacaatatt ccggcatcgg tagattattt cgagcaggcc
     5881 gcgggtgatt cacttgtgca tggctacgca ggttgggcta ttgcgtcatg ggctaacaaa
     5941 gattttacgc atatcgtcga acgtgcaggt tttgtaatac gcaaagaacc ggcattcgaa
     6001 tttgaattct acaaagatat taccatcagc accatgcgtc ttatgcaagc ctacggttat
     6061 tatcatacag gcaatatgtc ggcctgtctt gcccgcattc aagagttgga tggtggtttt
     6121 gccagtacaa cagatccggc attattgctg gcaaaacttg acgctttaag cgcgaccgct
     6181 tccggattat aaagcgaact ttttataaca aaaaaaccgc agcctaaaaa ctgcggtttt
     6241 ttttatgtgt aatttttaca gaagcatctt tttttcatca aaacgtgccc gtaaaaccaa
     6301 agtggatttt cccgtccgac ggacgatcgt tttgaccgag accatagtcc agagcaaact
     6361 gcccaagccg cgtcccaaat cgcataccaa agccataacc ccaggggaaa ccgtcttgtt
     6421 tgttaatttt cgtacgttct aaatcagcat atgctctgcg gtaataataa ccttgatcgt
     6481 aaaatacaaa caatcgtgat gcgcgccctg taacataacg caattccaaa ttattccaac
     6541 ctacgcgcgt tccgctaaaa aagccttcgg tataaccgcg cagagttttt aggccgccaa
     6601 ataaaaactg ttcactcagc ggcgccacat cacgaccggg tctgaacact gaaaaatgta
     6661 acccgtttgc aataacccag cgatgtaccg gttcacggta aatttccaaa tccatacgcc
     6721 atgtttgggt gcggatggat tgggaaaacg agcgaatgat tttttgctcg ccgccatagg
     6781 tgatcgtatc cgtcccgtcg gatgacagag tccaattttt ttcccgtgta cgtccatacg
     6841 aaaaaaccgt atgataatat aaaccattgc ggggattaat ttgttcgtca cgcatatccc
     6901 cggtcaagcc gccattccac gcgtaaccat cgctctgcgg tgtattaaat ataaacgcag
     6961 ttttcggatc ggcgggttcc accgactgta aacgcacccc cgctaaaacc gaccaatcgc
     7021 gggtaatttc ggtttgcaat tccatctccc aggcgctgcg cgaatacaac gaatcttcga
     7081 tttgctgttg cacggctatg cgtccgttta caggccatcc gactaaccac ggctcggtgt
     7141 actgaaccat cagatcctgt gaaaggcgat ttttcttttg ccagtgcata ctcaactgcc
     7201 gcgccgttcc aaaaagatta taaaacgata catgaatcaa cccggtgaag tatccttttt
     7261 catcacgcgc cgcatcgcgc gggacataac cgataatccc gtccattgta ttagcacgat
     7321 cttcctcgac atccagccat accgtcaaca gggaatccgg tctcattgta accgaagtaa
     7381 gttccgcact gcgaataaaa ggaaatcgtc ggatacgcgt aagaccgtct tgaatggcac
     7441 gatgatcata accgcggggt aatttcatgc gcatttcacg cgtcaaagtc agcggtttgg
     7501 tgctttcgtt tccccgaacg ataaattcgc cgaccggttt gacgatacgt cttttgatat
     7561 gcaacagaat atcaattata aaatcagact tcgcatcgcg tcgcatccag accgagtcaa
     7621 gcgctatatg tacaaacggc gcaccgttgt tttcaaacca gtccagcatg cgatttaccg
     7681 atgccgtcca ctggcccggc gagaacatat cacctttttt ccatcgcata acttccgtaa
     7741 tctgcgacgc tgaaatatcc gattcatcgc ttatgcgcac agagccaaat ttagcttgtt
     7801 tttcttccga cagaaaaaaa atcaaccgta tgccatctgt gccttcagct gatttttgtt
     7861 gaatggaatc aatggacgac gccaaataac cccgatcacg atagtattct aaaatacgca
     7921 tcgcatcatt gcgcgattgt tcgtgatcaa aaacggaacc gggtttgtga tagagcagag
     7981 ataagagtgt tttgtcattc acgatacgat tgccgtgaat ggaaacagaa gcaatacgct
     8041 ctgttgcggt ttgcgcaaaa agcgtagttg tgaaaaaaaa tataaaccag cgcataatta
     8101 ttaacgctct cataattgag cttatatgcg tattaacgaa caccaattct ctcacgcaga
     8161 gtcgcaaagt aaaaatgttg actgtgcatt gtctcgcctc attggttccc gctttccctt
     8221 cgacttcgtt cagggcatgc gcgggagcga aagagcagcg atagcgttgt ctcacccgcg
     8281 cacgcgggtg tccatatatt ggcgggtagt tttgtgttgc tacgtccagc ttcctctcat
     8341 tgggttccgc ttcccattgc ctggtgttcg atcacacata gacgtaactc acgccgagat
     8401 ctcgggacat atcgcgggaa caacagcgcc gttatttgcg tcattgcaag catgaaatct
     8461 ttctcgcaga gccgccaagc attgatgcat tgattatgcc ttgtcaaata tgaacgcctt
     8521 aatatcacaa tgggatcacc gtagcgcggc cacgggtctg atgggttatc gcatcgcaga
     8581 agtgcaaaac ttcatttttg cgaatcgcta catccatcgt ggcttgctcc gaatatgcag
     8641 tttcccctgc tttcccgccg gcatgatgta cacagcgcat gatcgtattg atttcatcgt
     8701 atggaaactg tatccgaagg ttttcgtaga tcagcctctg ttctgcacaa catcgcgaaa
     8761 gaacatccgc ggccgaatca ccgtacgcac gtacaagtcc gcccgtgcca agcttggtac
     8821 ctccaaaata acgaacgaca atgacgataa tatccgtcca gtttttagcc gtaatggcct
     8881 gcagaatcgg tttacccgcc gtaccggaag gctcgccgtc atcgctgaga cgaactgaaa
     8941 catgaggtgt taaacccaat tgataagcga agcagtgatg cgtggcatca aaataatttt
     9001 tacgaataga ttgcaaaaac tgttcggcgc cgtctttatc ttgaaccggc gcggcatacc
     9061 cgataaattt cgaaccttta actttgattt cggacgtaca tgattcggcg atcgtgtagt
     9121 attcatccgg cgtcatgcgt ttttccgagg gaatcacgat gcgtagaaaa tttttccgtt
     9181 atgtataacg ccgacggctt tgcctttcat cgcataatcg tgatacggtg tgttgattga
     9241 tttcgaacac gtctgatgca catcgtattt ccagcgcatt tccgtatcga gaatggtcag
     9301 attggcttgc gcaccgatga caaactccgg cagcggaaga tgcagaagtt tacggggtgc
     9361 gatagacaat ttgtacaagg ccgactccag cgaaatcacg cttttatgta cgagatcatt
     9421 gagcgtaata ccgacgcatg tttccagtcc ggtgatgcca aacgacgctt cgtccatcgt
     9481 acattcttta tcttcgatcg tatgcggtgc gtgatcggtg gcgataatat caatcgtacc
     9541 gtcttgcaaa cccaacttga tttcttcgat atctgcagat gtgcgcagcg gcggattcat
     9601 cttagcgaag gtattgtaat tttccacagc ttcgtctgtc aacgtaaagt gatgcggaca
     9661 cacttcacaa gtcactttga ggccgcgttg tttcgcttgg cgtaccagac gcacggttcc
     9721 gcccgttgaa atgtgtgcga tgtgaaccgg agcatccgta tattccgaga gtaagatgtc
     9781 ccgtgccaca ataacttctt cggaaatgct cggaatccct ctcagcccta atcgtgtgct
     9841 ggtgaatcct tcattcatcg aaccgcgatt agaaagttgt ttatcctctg catgctcgat
     9901 cacagggata ttaaacatct tcacatactc cagcgcacga cgcatgattt ccgcattata
     9961 caccgggtca ccgtcatccg taattccgac cgcgccggct tcgaccagac tcccgatttc
    10021 tgctaattct ttaccttcac gtcccttggt gacgcaaccc acgggatgca catccaccaa
    10081 atggccttgc gctttatgtt ttacgtactg cacggtatcg gcggtatcta tcggcggcgt
    10141 tgtattgggc atacaagcaa tagccgtaaa gccgcccacc atcgctgcgt tagctccggt
    10201 ccaaacggtt tctttatctt cgcggccggg ttcacgcaaa tgtacatgca tatccataag
    10261 accgtgcgta acgaccttgc cggagagatc aataactcta tccgcagggt catcgcattt
    10321 accgatgcgc acatagcggc catcggcgat aagtatatcc acgacttgat ccgatttatg
    10381 tatcagatcc aaaagtcgaa catttttgag tagcagtgtt tgcggtagtt tttctgcttt
    10441 gacgtcgcgc gccatgttag tgtttttcct gtgaaaattc gtaaataata taaatcatgg
    10501 tgactaaaat cagactgaat acaagggctt tcatctcacc gccttgtaca tcatatttta
    10561 tcccggtgat gtccgataat tcgaaatcac gaaacgaatc gtagatgcgc atggaaacga
    10621 tgccgtccat gtacgaataa aacacgccgc ggtgaatatc gccgttagtc gtacgaattt
    10681 ggatcacgta accgggtttt attttgtaca taaacgccga ttctatttct ttgcggcgtt
    10741 tttccttttc cgtttcatct ttcggttcat cggaccgcat atagtaatta tgcgaacatc
    10801 cgacgaacat catccatccg aacagcatca cccacagtaa gcgttgcgac atagagactc
    10861 cgtttcttat taatgcggtt ggttacgcat ggcatcagcg accatgtaaa aaaactcatt
    10921 gagcaaatca atcatccccg gctcatgcaa ctgttcgcgc atagcttgcc ccatacaagc
    10981 catccactgg tcgcgggcgt ttccattgat cggaaacggc gcatgacgca tgcgcaatgc
    11041 cggactgcct cttttttcca tatagaccgg cggcccaccg aaacggccca ccaaaaacat
    11101 aaacaattta tcccgcgatt cggtaagatc ggtcggatgc atcgcacgta tttcacgtgc
    11161 tgaggaatca gtatccatga tatcataaaa acgatcaact agtttcctca aaccttcttc
    11221 accgataacg gaatacaacg taccaataac ggctaactta tcctgatcca taccgcttta
    11281 ttttttctta cctttgcctt tagttttttc ttttaccgga tcacgcggca agtctttttc
    11341 ttgcggttta ttttgttttt gttctaccgc aggcgacatc gactgaccta aaaaattaac
    11401 attttcgacg acaaacggaa gctcgacgcc ttcgacatca tcatggatat gaatattgat
    11461 cgttccacgt ttgggcacgg cggtattcag attcaacacc atcgtcgtag attcttcagt
    11521 atacgtactg atataccgat ccgatatacc aaccggatta cctttttcgt caaggatatc
    11581 cattttattc aacttgaaat ccggcaaacc tacacgaatc accatctgat gcgtatattc
    11641 atcatcctga atatcttcaa tcaccgcacc gtattgattg ccttcggcgc ctttttcaaa
    11701 ctttcgaata ccaggatcca caacacttgt ggacgtgctg cgcatagcat acaccgttcc
    11761 ctcaatgcgt ttgatgcctt tggccgtagc atccggtacg gtcaatgtca gcgtccaacg
    11821 taaaaaatct ttttgtttgg acatttcgac gtagttggtt tcgcggtacc attcgctttc
    11881 atgcaaaaga ttttgtccgt tgtcggcgat agccgcggtg actacggcgc gttccgtttt
    11941 gacgattgcg cccggcaacg cacccgtaaa tgttacttga taactttgat ccgtaccaaa
    12001 ctggagataa tccggatctt cgacattgga ataggtaacg cgggtgattt tgacccaatc
    12061 aaaagttccg ccttgataac tctgcacggc acgttcgggc tttgaagact ccatcgtcgc
    12121 cgaagattcg gtttgattga ggccgagacg ttcgatcatc gcatcataac cggctttggc
    12181 cgcagaaact tcggcttcgt aattaaattg aggcgctgtt ttaccgctca ccacggcacg
    12241 caccgggcct ttttgaccga aaagtaattc gttgaccgca tcgccggacg gcggattgtc
    12301 tttgatattg gcgcccgcac gcgcttgctc tttaagccaa ttttcgtcgg caaataattt
    12361 atctaatacc tgaatgaact tctcaccggt aaaggaaacc tgatacgaac cgtcggcctg
    12421 ttgggtcata ttattagacg actgcgcttt gccgggcaaa cgaaaattca tttcgctttt
    12481 catcgtaccc ataatcgcca acagcatcgg acgcatttgg ttataaccgt ttttttgttc
    12541 ttttacggct ttggttattt ccgcatcggt ggtcggtatt ttttctgaac cggatttggc
    12601 tttatcggat ttttttgagg ggttcgttgc caattccagt acccagttct tttgtgcatc
    12661 ctgatatacg acgatgctgt cgccagctcc ggcattctta aattttacat tggaaatatt
    12721 tttgaaatac gccgtgcctt tgaaataatt gcgtccgtcg tcaagtacac gaaattcaac
    12781 atctttccat gtgtctacac ccgtcgcttg ctgaatgatg tcgcgcgctg ttttggaacc
    12841 atccggtgtt ttaccgctca tattgcccat catgccatcc ataccggcgg tcgtcacttc
    12901 gaccgtcact tttcctgaac cgtcgggatt gatcgtataa tcggctttgg tttcaaagca
    12961 accactccat acgaccgtag ccaaaatcat caacaataaa ggcatcatgc gcttcatata
    13021 ttttctcctg ttatatattt tgaatgtaaa acaaatacgc tcttacatca attggaaata
    13081 cactgttttg agatacaatg tttccggtat ctgaagcatc cacggatgat cgggtggctg
    13141 ataggtgata ttgactacac gcatcgaaac accattgtcc gcggaagcaa accgtgcggc
    13201 ctctagcatc aggtccaccg gcatgtgata cgcgcaagat gaaagtacga tatgcccgcc
    13261 cggattgaga agttggatcg cttggtgaag caacttccaa taagcccact tcagtttttc
    13321 gatgccttct ttgcgtttag cgatggccgg cggatcaata atgatcacat cgtatttttt
    13381 caccttgtcc ggcgtcgaag ttttgactgc aacttgctct tccatatact tgaatacatc
    13441 ggcgtggata aattgcaatc gcttctcgac attgttacga cgggcattgg cccggccacc
    13501 ctccagagcg gcggcatctt gatccacggc ggtcatacgc acaccggatt gggctaaagc
    13561 tacagcgaat ccgccggtgt aggaaaaaag atccagtccg gtttgattag gtttgactaa
    13621 tgtacgtaca tacgcgcggt tttcacgttg atccaaataa aaaccggttt tatgcccatg
    13681 atggatatca acatgaaaca acaaatcatt ttccgatatg acgatttctt ccggtatcgc
    13741 accatacaca acacccgaaa aaggctccaa cccttcttct tcacgcgatt ccatgtcgct
    13801 gcgatcatag attcctttgg gtttaacgac ttcgaccaaa gtttctaata tttcagtacg
    13861 aaatttttcc atccccagcg agcgaaattg cactacaaga tacgcaccga attgatccac
    13921 catcaaaccg ggcaagcgat cgccttcggc atgaatcaac cgccgtgcgt ccgatggcag
    13981 cgccaattgt gtacgaaaat caaaggcacg ttgaatacgc tgcttaaaga agtttttgtc
    14041 tatcggacga ttttttcgat ccagcacccg cacgacaata tgagataacg cgttataata
    14101 acccacagca agaaacttat cctccgcatc ggcgatttcg acgatagcgc cattggggat
    14161 atcaccgcga tctttgaatt gcgtggcgat atcatcacga aatatccacg ggtacgcatt
    14221 gcgaattttc ttttctttgc cggattttag acgaatacga tgcatgaagc agtccaagaa
    14281 atagcttaca taaatttgca ccgcaatcta catgaggaaa cgcagaatat caaaagattt
    14341 aaggtaaaca acaggtcaat ttaaaaaaaa tttagaatat gaattagaga ttgacgctca
    14401 ccaaaaacct gcatgtttat gtaggttaat ttcaaatcat tataaatcat attgttatgt
    14461 aaacattaaa caaattttga cgcttaaatc gtcatcaagt gtgcgttaga tcacatacct
    14521 cataaaaaaa atagtctatt atcattggtt atgataatag actatgaagc gtattagtaa
    14581 aaaaaacatt gctcggccgt cattttatag tagctttatg gtagctttat ggtaaatatt
    14641 tttcgtagct ctttttgcca tgaacataca ttatttattt aataggtttt aaaatcatac
    14701 atttcattaa accaaaggat tatttctatg cgtaaactta tgacgatgat tgccttatta
    14761 gcgctttgta gcgtacagac ttacgccaaa acatggaccg tggatcagag accaggcgcc
    14821 aattttcaga ctattgccgc ggccgttgct tcatcttccg tacttgcagg ggacacgatc
    14881 cttgtatctg gttcatccat tgcgtatgcc ggatttagct ttaacaaaaa actcacgatc
    14941 atcggcccgg gatatttcct tggagaaaac accaatctgc aagctgacac caattcggta
    15001 aaaatcagtt ctcaggttac gattcaaagc ggcgcgcaag gatcaattgt catgggcttg
    15061 catttcttaa acagcctata cgttgatgcg gacaatgtac aaattatacg taaccgcttt
    15121 caaattacga gtacatacga ctacggcatc tatttatatg cacaagacag taccattatt
    15181 cgtcagaatt atcttacaca aacagcatcg tggtatcact gcatcactat tgcttccggc
    15241 aacactcaga ctatgattca aaacaatttt cttgagtaca cgggtggaga ttctaattca
    15301 agttatggcg cgctcagtgt gcaagggacg atatccggtg acatttccaa taacgtcatt
    15361 tatggaaatg tgactttgtc cgcgggtggg ttttatttta ataacaatat tttgcgttat
    15421 gggaaattca attcaaccgg aataacaccg cataataata tcggaagcag ttcgcagttt
    15481 tctttctggc ctgctcttgg aaatcaaagc ggagttaata tgacgtcggt tttcgtatct
    15541 tccggcacat ccgacacgaa gtggcaaatc gctacggaag gcccggccga taataatggt
    15601 tccggaggtg ttgattgcgg tatgtatgac aatagcgccg gcacagctta tatcccgtcg
    15661 ggtattccgc cgattccgtc tatttatcaa tttgatgcat cggatctcaa taatgtcacc
    15721 gtcaaagtga aatcacataa ctaattctat ctgcaaaaaa tgcgcatcga attagttcaa
    15781 ccttcatttg aacgggagtt aacatgaaat tttttacagc ccttatcggg ctatggctca
    15841 gtgccacatc tttattcggg caaagcccgc cggatgtgca ctctgtcgag tacttcatag
    15901 ataccgatcc cggatttggc ttaggcactt ctgtagcacc gagtgcagct acttcgggtt
    15961 cggataagac gtttgtattt tccgtttcca caaccggttt accatcgggt ttccatacac
    16021 tgtatgttcg ttcacgggat aacaacgggg gttggtctca agcaacagca aaagcctttt
    16081 atgttatgcc cggcgaatcg ggttcgtttg caactgcggt gcagcgcgta gagtatttta
    16141 ttgatacgga tcccggcttt ggcaacggaa tagcgatggg atccccctcg tcgggagatg
    16201 cgacggtgac aggcatgatt gacatttctg cactccccgg cggttttcat acattgtacg
    16261 tacgggcacg cgacggattt cataagtggt ccgtactcaa tagtcgtact ttttataaat
    16321 cggtagtata tacccccccg acggatccgc aagtcacata catcgaatac ttcgtagata
    16381 aggatccggg atatggtaac ggaacatccg ttccgggatt tacggcttcg tcggatatca
    16441 cacacaattt tttagtggat atcggtgcgc tttcaggtgg atttcataca ttgtatattc
    16501 gcaccaaaga tgccaatggt aattggtcta tcgcatctac gaaagccttt tataaaccac
    16561 acaactatgt tgctccgtct gatcctcaga tcacggcgat ggaatatttc atcgacaccg
    16621 atccgggttt cggcgcgggt acggccttga gctttaccgc ttcatcggat gtgacggtca
    16681 acggcgtggt agatattact tccgtcagcg ggggttttca tacgctctat ttgcgcagca
    16741 aggatgccaa tggaaactgg tctgtcgcgc aggcgcatgc gttttacaaa gcgcataccg
    16801 tctccaatac atcgtctaac atcacagcca tcgagtataa tttttacaaa tccggatcat
    16861 cgacaccggt acggacattt acggctttta ctccttcaac ggatgttcag acgacgtttc
    16921 tggctgatct atcaggtctt aacatcgaca gcacctacga tatgcgcgtt acggcgattg
    16981 acgcggcagg acgtcgcagc attgcggcta cggcatccac cgtcatcatc gaacgtccta
    17041 atcatgcgcc ggaaattaaa tcatcggttg ccgatgtgac cgccaatgaa gactttgata
    17101 gcctgatcgt cgcgtcgctc gacacggtgt ttcatgattc ggatatgacc accgtaggcg
    17161 atcatttgca attcaacgcc tcggtcaacg gctcagccgt gacttctgtg ttgcgtggtt
    17221 cgcagctggt aattttttct tctccggata tattcggatc cgccgaagtg atcatcacgg
    17281 ctaccgatga cagcagcgca tcggtcagcg acacgataca tgttaccgta aattccgtta
    17341 acgaccggcc attggcaacc tacgatgtcg cgcaagccaa tgcaggatat acggcgtatg
    17401 tccaaccgct caaaaacgat acggatattg acggggacgc cctcgtcatc gcagcgatcg
    17461 tcgcgatgcc gcaacacgga acggctacgg tagtaagccc ggcggatacg ttgatttcct
    17521 atacgccgac agtcagttat agcggtatgg actcgatcca atatgcggta agcgatgggc
    17581 tcggaggcct tgatacagcg atcatttatc ttcagatcaa cggttatatc aattcagccc
    17641 ctcagcgaaa caacaccatt gccgatgtta ccgcagcgga agatttcggc gttatcgcat
    17701 tagcacaact caattcggta ttcggcgata atgatatagc gctcaacaac gatagtttgc
    17761 ggtattccgt ttccgtacaa ccggccatcg ttgcattaag tttgcggcac gatacggtgg
    17821 ttgtcaattc gatcgctaat ctattcggca tcgcggaagt tatcgttacg gcgaccgatg
    17881 acagcggcgc tacggccagc gatacattga ctgttaccgt taattccgtc aacgatgcac
    17941 cgttcacaca aaacgacgcg gtttcaacga cgggtattca aacggtcgta gcgcatcctt
    18001 tgaataatga caccgacgtc gagagccaaa cgcttacgat cgcggccatc ataacgtcac
    18061 cccagcatgg ttcggcgacg atcccgtccg gtgatacgac gatcgcttat accgcaacca
    18121 tcggttacag cggcaacgat tcgctgacgt attcggtcag cgatgggaac ggcggtttgg
    18181 atacagctaa gctcgtcatt acgatacaag ctccggcgcc gttgagtctg agtacatcgg
    18241 tccaccaaaa tccggctttg gcacaatacg ccgacgtcta tgtcatcgcg gatacggcgc
    18301 tcatcgggtt gcccgacgtc aaaatgtttt tgggaagcga ttcgaccgcg ctgacgatga
    18361 ctgtatctac atcgcgggtg tatcgcggac aatttgaatt tgcaagcagc ggcgcttata
    18421 cgattcgtac acgcgctacg gccgccaccg gcgcgcaggc tgtgaacttc cgttccttta
    18481 cggcgacttt ggccaaaccc ggcgtgacga caaacatcgc ttcgaccgac gcccaagccc
    18541 agcttttggt gaaatcgaaa gccattgttt caccgacgta catcaccgtt gcgacggatg
    18601 aatctatgaa cggcaaggcg tatcaattcg cacccgcatt gacactgcag gaaccgtcgg
    18661 aaataacgat acggtttgaa ccttcgcagt acgaagatgc cggtaaaatt tttatcgaaa
    18721 gcgaaacgga cggtcgtatc gtacgtctgc aaaccggggt ctatccggag caacgtatag
    18781 ccaaagccaa gatcacttcg ctcggtaaag tgcgcatcgt gtatgatccg tcgttcaccg
    18841 gttccaatac cgttccgaca aatttttctc tgaaagccaa ttatccgaat ccgtttaatc
    18901 cgtccaccac gatcgcttac gatctggcga aagaaggtct ggtgacattg acggtgtaca
    18961 acgtactcgg acaaaaggtc aaaacactgg tgcatggtca tgcacctgca ggttcgtaca
    19021 aattgctgtg ggatgccacc gatgaacgcg gcaaacgcgt ttctaccggc gtgtattttt
    19081 atcgtttaga aacgcccggc tttgtacaaa cccgtaaaat gcttttgatg aagtaggagt
    19141 aatcatgaaa aataaaatta tcttttgcgc actggcgatc gtgttgttat cgagttgcgg
    19201 tcccgatccg ggaacatctg aagattatgc ggccacggta gccgaaggct ggacattttt
    19261 tgaaaccggt tcctatccgc aagcgaaaga acgttttcgc gcggccatcg cgatagaccc
    19321 tgctttttca gaagccttta ccggtttggc atggtcacag atgaaaacgg acagcttaga
    19381 tgaagccgtc actgttttta gctttggttc ggctacgtcc gacgccaccg ccgatttatg
    19441 ggcgggatgg gcgtttacgg aacatgcgct gaagcagtac accgcttcca atggtcgcat
    19501 cacgaatgcc atagccgcgg atgcgaactg gatatttagt cataccgcag gattatcggt
    19561 cagcgatctt cgcgttttga cggcagaaaa tcactttgca ctcggcagtt atgcggccag
    19621 tctttcggcc gttcagcaac tcaatgcggc ttttaccgcg gatgtgacga ccaccgccgg
    19681 tcagtctgcg ctcgccgctg agattgagcg attgaaggga ataaactaag tctttatctc
    19741 aagggtcata aaaaaaggcg aagtgcaaca cttcgccttt tttattttca ggatgtgatt
    19801 tgcgttcggc gggattttta gcactgaact ttcatcgacg aacagcagct gaattttgcg
    19861 cttcctttga tacgaagccg ttcagcccgc ctattgccaa tacgatgtca gcggctggca
    19921 atttttccaa gttttaaaat tagtttccaa gttatttctt tttttgtttc aatgtctttc
    19981 caatgttctt gcaacttttg cgacaagtgc tctataggat tttcattata tttgtcaata
    20041 tatttttgag ttgcagacca acttcttaaa tgtcctaatg tttccgaaag tgttactagc
    20101 cttttaatga aaaaatccgg tgtttttatt tcgtcaaaag gaaatggtat tgttaaatat
    20161 tctttttcaa tcagtctgtt ttcgggaagc caaaattcgc cgacaatatt gtcgtgaaag
    20221 tttttaatga tcttgtctaa ttcttcgttt attgtcggaa taccataagc ccaaacggca
    20281 attattccat ttgtctttaa aactttcttt acttgtgtgt aaaatttgtc gaagtcaaac
    20341 caatggattg cttgggcaac tgtgattaag tccacagaat tgtctgctat tgcacctgga
    20401 ttttcggcat tttcaacttt atagactatt ctgtcgtgag gaatagcatt ttttatttgt
    20461 tcttggcttg ggtcagatgc ataaactttt tcgtagtaat cagtcaattt tatagctgat
    20521 tgcccgtttc ctgttccaca gtcccacgcc aatttatgtt ccgcagtcaa accttgtaaa
    20581 aactcaaaca attcttttgg atatgtcggt ctaaatttgg cataaacttc tgcctgcttt
    20641 gaaaaattgt ctttaaatgt gttcaatttt ggttgtcgtt ttttacttgc cgctaacggt
    20701 ttgcagcttg gcgaagcggc ggatatttgg tactaaagtt caatagaatc gctactgttg
    20761 agccttgcaa aaaagtttca tggatgcact gcacccgcca ttttgcaaaa ccaatgttag
    20821 tggctggcat tattacgctc taatattttg tcaaagtaca ataatgtttt ttctttgtca
    20881 tttttttcaa acatatctgt aattaaatat tgtaaactgt ctggtgttgc ttttttcaaa
    20941 taagtaagca tttctataga tgttgtatag ggtggcggtt ggaatttaac tttatccaaa
    21001 tattctttta acgcagtatt taatttttca actccaataa attcgctaag tgtatagagg
    21061 gcaatagctc ctttcccata agcaatgtag gactgttcag gatttactaa gtataagggc
    21121 ggttcttgtt ctgtttcatt tgccctgccc aataaatatc gtttacgctg tatctgcaaa
    21181 aatttcaacg cactttgtat gccatatttc ttttcataaa tttttgcggt aatgtattca
    21241 gctatacttt ctgttatcat agttgcacct aaagcattag caggtattac ttgattgccc
    21301 caccattgat gcgaaagttc gtgtgcagca acataaaagg aaaggtcaat tccatcttct
    21361 tttaaattac gagcatcatt gataaaacgg atttcagaaa tggggacaca gtttcctgct
    21421 gtagtcgcat aagaaccttc agaccttgaa aattcaataa tatgaacttg cttgtattgg
    21481 taagtaccaa aatgcaacac attataatct aacgaagctt ttaatccgtc catcatttgc
    21541 tgaatgttgt atatgtgttt tgggtggtaa taaacccgta aatcaactcc tttatagttt
    21601 tcttttaata ccgcaaattc gcctgagtta aagccgaaaa caaacttgaa gggtttgtcc
    21661 attttgtaat gaaagtatct tttgccatct tctaaccatt ccctcaataa atttcccgat
    21721 gtaatcgcaa tttgttgagg gcttgtacta acaatagcct caaaatttac taaatcggca
    21781 tcaatgcttt ggtaatgatt ggcaaaagag ctactgttgg tgggtatctt tttttctgta
    21841 tcagcgaaat atcccaatcg tggaaatata tcggatttta aataagttcc attttttaaa
    21901 atgttggaat tttgagttaa aagcgtattc ttttggtttt tgattgtaaa gtttaagtta
    21961 atgctgtcat tgggtgaaac tcctttatca agcttataca tacaaaactc aaaaatagtg
    22021 tcttcttcaa ttaaagcagc ttgttgatca aaggaaagtg tggttatttc atcgaatcct
    22081 ttttttatta aaagcgtgtc aatggcatat tccgttttat ttatgagggt atattttcct
    22141 tttgcaatga aagaattagt ttcgggaaaa atgtctaact taacaaagac ggatgtaata
    22201 cgtggttgtt tagtatgctt gtatttcccg tatttttttt ggaattgact taataagtta
    22261 tcttcattct ttacggataa cattttgttc tttggggcat tttctttttg aaaaagaaaa
    22321 aaaccaaaga atagaaaaat tatcattaat acaattgata taaatgccaa cttgccagca
    22381 aaacgttttt tagcaatttt aagtctttca tgaatagaat ttgttagctc tctttgccat
    22441 accaagagtg taatacaaaa caaaaacaat ccgaagacca accaatataa tttatacaag
    22501 aagtatgcag atagtgaatg tccatagcca ctcatatctg aatatttgag gaaaaaatca
    22561 gaattggggc tttcattgaa tctaaaagca aagttttcta tgcccaaaga aggcagttgt
    22621 gaaaccccta aaacaaacaa aataagaagg aataaactca aataaacatt gccaaacatt
    22681 gactgaacaa acaaagaaag gaaagcccaa attataaaac taatcagatg aattaccaac
    22741 aaatcaaaca aataatgacc aatttcataa tggtaataat tgctgtatgt ttgaatggcg
    22801 atacctactg ccattatcaa tgaaagtaat agtagttgca ttttaaccaa tgccaacaat
    22861 tttgaaaaca gtaaaaccca atttggaacg gcagtaacag agatcaggtc tgaaatcttt
    22921 gaattttttg ctcgatgaac taaaatccct gcataaagaa aagtaaggat ttgaataaga
    22981 aaggaaaaaa agaagacagg aaagcctaaa accacccaag taacgggcag tattttaata
    23041 tctgtttgtg ggtttatttg caataaaatt gcgacaataa atatgatacc tgcaataaca
    23101 atactgataa aagccccgtt ttttatgata aaataaaaat cagtttgaga taatttccac
    23161 gatgttttga tttgctgaaa aaaggaaaaa tcaaattttt gttttatcag gtttattttg
    23221 atgatacttc caaaattgtt ttttgttaga ctttcacttt tgcttgtttt aaaacagaat
    23281 gaaaatggat tttggctaaa tgaaaaccat cggaaggttg cgataaaaat gaatgctcca
    23341 attaataacc aaacaaacct attgattaaa acaattggtg acaagggtaa ggaaagcgta
    23401 ttttgttcgg ataaactcca ataacgagta aaatattgca ctgcactttc gccaaaaggg
    23461 tctatcagca aacttgccaa accttcaaca cctaccaatc gaatggcggc ttctttaaaa
    23521 agccaaatca aaataatgga aataaaaccg gtgtaaatat tcctactaag caacacaatt
    23581 gaaaaaacga caatactaaa caataataaa ttggggatta agtaaataaa atagatatgc
    23641 aaataaggta ctgcatcaaa aggaagtact tgtattggat taacggttgg cagttttgta
    23701 ccaaatatta aaccaagcaa aacagaaaag gcaattaagc acaatatcaa aaaagcactt
    23761 gtaaatttgg caaataaata atcggtctta gaaaatggat aggtatgcaa aatgccctta
    23821 aagttgcttt tgtagtcttt ataaatagta ttgccgacta tggcaggtat tacaaataaa
    23881 agaaatttgt taaagaaatt agcaaaccca taaatactca taggggaatt agctatattt
    23941 tcggcagagg cactgccttc tccaaagcct cccgaagcac ctgccatact tagcaatgac
    24001 attaggaaga aaacggataa ataagtataa aatgaccaat tttttagcca atactttatt
    24061 tcaaatctgg aaattgctaa aaacattatt ctaattttta aggttgtttt ttaaggcaat
    24121 aaaataaaca tcttccaaat ttggcgttgc tgatgcaaaa ttagatgagg gttgttcatt
    24181 ggcataaacc cgaatattta cggaattatc ttggttgtat ttagatgaaa gcacaaaaaa
    24241 agcttgttct gcttcttcaa gtttttccct atcaatatta cttgtccaga ttttactttc
    24301 tatttctttt gttgcctctg agggcataat ttttttcaat attttaccgc cattcattat
    24361 tgccatatca ttacacaaat ctttaatgtc atctacaata tgggttgaaa aaatgataat
    24421 atttgaacta gcaatttctc gcaaaacatt taaaaaacgg ttccgttccg cagggtctaa
    24481 gcccgcagta ggttcatcta caataattag tttgggattg ttgagcaaca gttgagcaat
    24541 accaaatcgt tgtttcattc cacccgaata gccatcaaca ctcttttttc gcacttcata
    24601 aaggtttgta atttccaaca cttgttttat caatgcattt cgttcttgtt tcgacgcaat
    24661 acctttcagc gttgctaaat actctaacaa atcctctgct gacattttag gataaacccc
    24721 aaaatcttgt ggtaggtatc ctaaaatttt tcttaattcc attgggtctt ttaaaatatc
    24781 tatgtcctca aaaaaaacac tcccctcatc agagggttgt aaggttgcca gtgtccgcat
    24841 taatgacgat ttacctgctc cattaggtcc aagcagccca aacatacctt gctttatttc
    24901 caaattaagg ttgtccaggg cttttacacc attaggatag gtttttgaaa gattatttat
    24961 ttttaaagtc atttgttatt cgtgtttttt agatgacgat ttaatgtcga tattgttaca
    25021 gtcggttgtt tttttatgct tgccactaac ggtttcgggg cttggcgttc gggcgggttt
    25081 cggaacacaa aactccaagt ttgcacgtca gcccgcctga cgcaaaaccc gtattacctg
    25141 ccgttttttt atgttttagc aaaatcaatc aaaggcttaa tatcaccaga atctgcttgc
    25201 cttatcgcag tcaaataaac tgatctcgct tctccctttt tatttagatt tgtgctattc
    25261 caagtaaata tcggttttcc aaataattga ctaataatga tatctgctat taatcttgaa
    25321 tgcctaccat taccgtttgg aaaacaacgt attttaacaa ttctatgttt gaatcttatt
    25381 gcaaattcct catcagtata tgttttattc tttaaccaat agagactatc atccaataat
    25441 tgttttaaag aaaatcctat ttgatgctta tcgactccaa tatttttgtt tgtctttcta
    25501 aaattacctg cccaagacca gacttcgtta aacattcttt tatgaagttc ctttacaaaa
    25561 atttcggata ggatatactc aggtttccat ttttttccaa tagtccactg tacagatttt
    25621 tctatattta attgttcgaa ttcgtctaat tcaccacgcg ttgtgattga ttttattaac
    25681 agcccttctt tctcatcttc atctaaaggt gtttgccctt caatatattc tatttctaat
    25741 cccataaata ctttggcatt ttagttttga tttcttcagc tctatttttt attgcttttt
    25801 ctatacgaat ttttgaattc tcctgatcct caagttgcat tgtgtatgaa gttctcatga
    25861 caatatcctt tgcaatgtct aaagctcttt tttcaatcat ttcttcaata ctttgtcctt
    25921 ttggtataaa cccataaaca agtttcatat caagtaccgc tccaacctcc ctaaggcctt
    25981 ttattgtaat tgaaccattg gcctcacgct cctcaatctc ttttacactt tgaggagata
    26041 tttttaaacg attacccaat tgtctcaacg acatttttaa agcaatgcgt attgtatgta
    26101 tccatccttt ggggggtatt gaaaatgaac tcaaaggtct gaacaatgcc aattttctat
    26161 cagcttgttc aataattagt cgttgttttg aaatatccat caggttatat cctaaaatta
    26221 tttactaaaa ttaaggttat aatatgaaat atcaagatat tttttaaggt taaaacctga
    26281 cgataagctg ttttaaaaat ggggggataa cgttttgggg ctacaagaag ttggcgattt
    26341 cggagcacaa aactgtc
//
LOCUS       DAOQNQ010000015        26098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_626_length_26098_cov_5.769573, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000015 DAOQNQ010000000
VERSION     DAOQNQ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 26098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26098
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_626_length_26098_cov_5.769573"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PK754_01990"
     CDS             complement(<1..680)
                     /locus_tag="PK754_01990"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNB08308.1"
                     /translation="MNQHQRMYWLCQLGGWLGYAAINCLFMAGFYHLTVPLVLANVFV
                     SAMGLIGTHAFRSAIRRHDMLSWSLLRLIPIILFKTLLVAAIMECGVFLSSHFVPDAP
                     REFRFAVTLARMFNFSVVALLWSVIYFSIHFFQDRKNSQIKNLKLESALKDAELMTLK
                     SQMNPHFLFNALNSIRALAGSDGARAQEAITKLANILRYALQNAAAPEVTLQDELQTV
                     QDYVALEK"
     gene            complement(735..929)
                     /locus_tag="PK754_01995"
     CDS             complement(735..929)
                     /locus_tag="PK754_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015299891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08309.1"
                     /translation="MFKRLCQNVKHRPGFAVSAGIGIALGASLNDLAMGLAIGMLIGY
                     GMLSCRCMIFKKRTPNPTAQ"
     gene            complement(974..1858)
                     /locus_tag="PK754_02000"
     CDS             complement(974..1858)
                     /locus_tag="PK754_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB08310.1"
                     /translation="MRLYSISWLIAAVLASLLLTVSGHTQTRTDFGQVIVYGQGPDII
                     LIPGLGCSGEVWKTTVGQIEKTNTCHVVTLAGFAGVPAVSGDFLAPRRDALVAYIRKN
                     CKTKPIVIGHSLGGFLGLMIASEYPDVIGKLIVVDAVPFLPALANPAATVELAKPQAA
                     MMERMLLNQDAVNFAQSQYATIRTMVSDSTQSRTVAEWSIASDRTSLTAAMREMMTTD
                     YRKNISAIRVPTLVLGSWGGYKTFGMTSDMVLGLFQQNYAAQPSVTIHMAPTARHFIM
                     LDEPNWFFSEVLSFLKTR"
     gene            2428..3480
                     /locus_tag="PK754_02005"
     CDS             2428..3480
                     /locus_tag="PK754_02005"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNB08311.1"
                     /translation="MNYIFVLWTTLIWGMFAALFVLQSYFFSIAIGQSFDLWLRLSFE
                     GSQALIWAILTPVVVSFAKRWNLHGASAPASLSVHFVLGFVIGYIHRAASFWLYIALY
                     PPDTPLPSSVAWAKILGSTMNSVLIYWCIVAVGYVVHYVRAHQQERLQAAHLESLFQQ
                     SRLESLRRQIQPHFLFNTLHSISALMDDDVPGARRMITRLSDLLRKTLDQSERSKIPL
                     GEEIRFIADYLAIESVRFQDRLQVRYEVAPEVEKALVPSLILQPLVENAIRHGVAHVA
                     SPSVIIIRALRNEENLNITILNTSVPVNELEKGIGLKNTESRLQLAYGERAALKILTI
                     QDQFSVTIEIPYEDRV"
     gene            3464..4234
                     /locus_tag="PK754_02010"
     CDS             3464..4234
                     /locus_tag="PK754_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNB08312.1"
                     /translation="MKTEFDHELMAVVADDEVLARKRIRHLLAKRSDIRVVAECVHGY
                     DVCDKVLEYKPDLLFLDIQMPEMDGLEVLRTLGPVAVPGVVFVTAFDQHAIRAFELHA
                     LDYLLKPFDDARFYEALDRAIQMIGKQQLPDNLKRLLQNLPRPEAFMPRIPVKDGIRI
                     HFVETANILWIEATGKNTMMHTTDGSIKTAKNIIEVARDLDPTQFYRIHRSYIVNIRA
                     IREMQSWHKGEYVVILSDATRLVTGRAFRGNVDLLMRR"
     gene            4451..5596
                     /locus_tag="PK754_02015"
     CDS             4451..5596
                     /locus_tag="PK754_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08313.1"
                     /translation="MKTFIWLWLTITCIGSTSCRDRSAGVQEPNALSQDDKDVLFQIH
                     NGDLAGAESKINAMLSNNADNLRGHFLKTSLYFHARYFASQGASRDSITTLLLKSCEE
                     AITQAEKQSESVEQQFYLGSFYSFKSRALLAKREWTSAFFAARTGKNLLESVIESAPG
                     YADAYLPLGVLYYFASTQQAWWQGALAWVADLDVPKTQALTYIQKTYDEGLLNKNEAH
                     FVLNNINLFFENKLDIAESLLQNYNAQFPNNRFFLAPMQGLKLTRIAEEKGGEFLLTH
                     LDSLVTAHPLNGMQTLNRVGYRYLGQKKFPDALAVFHANVKLFPGSANCHDSLAECYY
                     TMGQMQESKKYYSLALTKLSGDQTINEEFKKTLKEGIEVKLIDLASY"
     gene            complement(5653..5726)
                     /locus_tag="PK754_02020"
     tRNA            complement(5653..5726)
                     /locus_tag="PK754_02020"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5690..5692),aa:Val,seq:gac)
     gene            complement(5739..5824)
                     /locus_tag="PK754_02025"
     tRNA            complement(5739..5824)
                     /locus_tag="PK754_02025"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5788..5790),aa:Leu,seq:taa)
     gene            complement(5838..5911)
                     /locus_tag="PK754_02030"
     tRNA            complement(5838..5911)
                     /locus_tag="PK754_02030"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5877..5879),aa:Cys,seq:gca)
     gene            complement(6034..6106)
                     /locus_tag="PK754_02035"
     tRNA            complement(6034..6106)
                     /locus_tag="PK754_02035"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6071..6073),aa:Gly,seq:gcc)
     gene            6401..7276
                     /locus_tag="PK754_02040"
     CDS             6401..7276
                     /locus_tag="PK754_02040"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNB08314.1"
                     /translation="MNSKKALIIVFVWVFGLSSCALFIDPYEAQYRQLLHERDSLQLS
                     IARTRHDLKDLEAELWKTLYDTEYLRNALVLAQADSVKNFYYNYVFRYRRPIQAIAEI
                     DPASPFLFPLKTEKDQNRIKFRIMWGRLFYFDFNHLGIDIRALEGDTVRAIYDGVIQN
                     YGGAEGYGELVAVVEHEYRGLWNKDVVPQRFMSIYGHIRNRCYDGEPELKWKAGDRIR
                     KGDIIGFINDDSRNGYGMEHLHLGIRRQSAMDAQAVDGGYWMRGYDTPKNSKFQFFMN
                     PIDLFGRYVQFVLPE"
     gene            7373..8260
                     /locus_tag="PK754_02045"
     CDS             7373..8260
                     /locus_tag="PK754_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HNB08315.1"
                     /translation="MRLITRTDLDGLTCAVFITIMEKIDEVVFYEPKEMQDGKVPVTD
                     RDIIANLPYHPNCALWFDHHQSQLIHQNAEYRGKFAVAPSCARVIYDFYEQPDELKPF
                     KELLEETDRVDSAQLTMEDVMNPKGWVLLSYTLDPRSGLDAFTDYFQRMIGWIMVHTV
                     DELLQLPDVKSKVDKYLADQDKFKEALTTYSREDGNVIITDQRSVDKFPSGNRFLIYT
                     LFPKANVSVRLFKGKEPGVTVCAVGHSIFNHSCKTIVGELMHEYGGGGHKGAGTCQFP
                     EAEAEAKVAEIIERLKKNG"
     gene            complement(8375..9403)
                     /locus_tag="PK754_02050"
     CDS             complement(8375..9403)
                     /locus_tag="PK754_02050"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNB08316.1"
                     /translation="MITIHVLPTWAQNNIPEWSWSGFVDAYYGYDFSKPVSGDRPGFL
                     YNHTRHNEFNVNLALLRVQAKYDEAYARVGIMAGTYAQSNLSAEPATYQHLYEAYTGI
                     QINAKTSLEVGLFGSHIGWESAVSKDNWTLSRSLAAENSPYYLSGVKATYSLSEKWTF
                     AAVVCNGWQRIYRSSKNVTPAMGTQIVYKPSDRLTINYSTFVGNDKADSTRQWRYFDN
                     AYAIWQVNPKLGITTGVDFGAEQKSKGSSKYNTWLTWAIITRYMLNDMWSVAVRAEQF
                     LDKNQVIVSTGTVHGFQTLGLSANLDYALTKQSVLRIEGRQLQSRDAIFSGETSNLSV
                     LSSLAISF"
     gene            complement(9456..9998)
                     /locus_tag="PK754_02055"
     CDS             complement(9456..9998)
                     /locus_tag="PK754_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNB08317.1"
                     /translation="MKALRLLLIVLGILIALPLLVALFVKKQYAVERSITINQPNQVV
                     FDYVKFLKNQDHYSKWATMDANMKKTYRGEDGQVGFVSAWESQNPDVGKGEQEIVKIA
                     DGERIDFELRFFEPWQSKEPAYMTTSAATDSTTLVKWGFSGHMDYPMNLFLLFMDMEK
                     MIGDDLATGLANLKTVMEKK"
     gene            complement(10073..10300)
                     /locus_tag="PK754_02060"
     CDS             complement(10073..10300)
                     /locus_tag="PK754_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HNB08318.1"
                     /translation="MEPFHDFGKTLMILGGVLIAVGMFLTFSDKIPLIGRLPGDIFIQ
                     RENFSFYFPITSGILLSVILSVLFWLFRSKY"
     gene            complement(10312..11139)
                     /locus_tag="PK754_02065"
     CDS             complement(10312..11139)
                     /locus_tag="PK754_02065"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNB08319.1"
                     /translation="MSPLTAQIAEAVNYIQSKAKTQPSVGIILGTGLGGLVQEIRKEI
                     EIPYQDIPHFPLSTVESHAGKLIFGTLGGKHVVAMQGRFHFYEGYTMQQITFPVRVMK
                     FLGIKALLVSNACGGMNPQYQPGDIMIMDDHINLLGDNPLRGKNEDTLGPRFPDMSEP
                     YSRTLIALAENIALEEKIKVQKGIYVAVMGPNLETRAEYRFLRYIGADVVGMSTVPEV
                     IVANHMGIPCLGFSIITDECFPDSLQPVDLPKIIKTAGEAEPKMTLIMKRVVEKMNL"
     gene            complement(11136..12020)
                     /locus_tag="PK754_02070"
     CDS             complement(11136..12020)
                     /locus_tag="PK754_02070"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HNB08320.1"
                     /translation="MKLNPGDIQHQEFKRVMRGFDPDEVTAFLEVVAENYKEILDETK
                     LLQQKLKAIQDETQYLRLRIQSSEKSLNDYKQEMVKVEKLMDARVDADLILEQARAEA
                     ERIRTQAQKENESLAQEVNFLQLQKTKVAAHLREYLKSQIALLEIVGDQKAEAAAKTI
                     PVPEKVEPRVEKPVEIKETELPPPIIPVATEEELKAGLEEGIGSFVQDLKLDDLPPEL
                     AAAIRESADDTAITDPERHEKKKKVIEELEKLTHNATGIFKKSDFEKMLGDSADKKSH
                     ELLDQIHKELEKKRSEKS"
     gene            complement(12017..12790)
                     /locus_tag="PK754_02075"
     CDS             complement(12017..12790)
                     /locus_tag="PK754_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNB08321.1"
                     /translation="MDIALQTTIRANIETIRKKIEASARKVGRDPKEIHLIAVTKTYG
                     IDYVREAVAAGISLIGENKVQEAKEKYTELEQSGNVPDFQLHLIGHLQTNKARQAVEL
                     FDMIHSVDSFRLAKEIDRRAYTVDKTMPILIQVNTSREATKYGVEPDNTLELIKQVSE
                     LKNIRIQGLMTIGALTAVQANDPEKIRSYFRRLRELRDFIREQNIPNVEMNALSMGMS
                     SDFEIAIEEGANYIRIGTALFGKRKRAAKSAAPKSEETL"
     gene            complement(12811..13863)
                     /locus_tag="PK754_02080"
     CDS             complement(12811..13863)
                     /locus_tag="PK754_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08322.1"
                     /translation="MNVRSHIASIQIGILVDFQIYTAYLCFCIQTMNRIVFSMSTAEH
                     ISPAASAMTETPDSVFRSQRILALDPQVFNSEEIQVIHLSLLQPDAFRELLDRFNYHQ
                     LNNLLDALIKDGILYYQEKHGVTTTERTVRTRCENLLDYKERIGCVEPVESCLHEGCI
                     HLKPACAVRKVKEDVLNVARLFEARKNRSTVPHVAIELFFKKLIEDYNCVGLILADKW
                     GTPKVFTTDGSGVLSVISEGSRRLTDAITDREFRFTKTGDSYQVFNQKFHIDAKLAER
                     LHAVEKNPDLTKFIKRITFSSIGIEVAHEKIILSVLYMGDNLLHQFLGKTISGIERIK
                     LETEPELRIRAGFGRK"
     gene            14076..14768
                     /locus_tag="PK754_02085"
     CDS             14076..14768
                     /locus_tag="PK754_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08323.1"
                     /translation="MNKRWMIFVVWCCAGVLSAQQYQVRISPDTILCSDQEAIIRVSP
                     GRSGVELTAVARKGGGSIGDFRYANSDQGYVNQAIFSSPFSGEIEIEVMDNKNNKVGM
                     VTVTVVPPTMEFMEDESIAESNAAEKRIPLKIKVLDQNGSFIKTAKVTCKVSEVEGTG
                     KNKKIKPSDAKVGAFVLLDGYYEGQLTNLKNATYRLEFFDEAHLLPFDSEENPDAQHP
                     SLLIDSWPISFN"
     gene            14922..16661
                     /locus_tag="PK754_02090"
     CDS             14922..16661
                     /locus_tag="PK754_02090"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNB08324.1"
                     /translation="MIKPFSLRLDEPRGWGFVLLLLCGCATIGAPGGGPEDKEPPTVI
                     TTFPAKDSTGVSRQTEVTVTFSEAMNKASVEKALFIAPFPARDPDLSWDDATLTIRFK
                     DALEENRTCVITIGTDAMDAHQNKFKSAWSFAFSTGDKIDAGSIAGTVVSESKTKPSV
                     WAYRLNGHTSSRDSMIYTKRADYITQVDAQGKYRFSYLADGAYRIYAVSDQGEDFRYH
                     PMQDMIGLAPRDVILADTSKEALFVDIAVFREDTTTMAVQLAQMTGPNEVLIRINNVL
                     AESRYDGTKIGSEHIASHFSIRDSLSGTDVAIRDVYFVTTGATEEIRLLCDSMSSQRV
                     YVLDVRGIYGLSGDSIAFQQINFRAEGGFETLRAKIEWTFPYGTPGLIAVNDFIGWKS
                     DRGLDRPTWEKAFVLRDTLGNSVNGTFFWMHSASGKFIPARNLRGRMPYEVHVDGKIL
                     QDWSGRPAFDTTMVWRLVAMPEDTVGTVSGMIADESSDDRGMYHITARNLNRSGRDYT
                     LIVPSPGGFTLDGMLPGKYNFLAFRDRDSNGVFSFGQTAPIIRPERFTSYSDTVTVRK
                     NWETSNIQIRFRK"
     gene            16665..17075
                     /locus_tag="PK754_02095"
     CDS             16665..17075
                     /locus_tag="PK754_02095"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNB08325.1"
                     /translation="MKSIYYLHQIKTGKNFRVEENQEVTIGRAFDNTLMIDDNSVSRY
                     HAQIKWKKNLMYITDLQSTNGTSVNGEKVEHGYFTELNFSDLVKIGNIELKILDEESV
                     IGKNFTPDQAPAKTIVINQAMLKKAVDPDDFTHN"
     gene            17092..18708
                     /locus_tag="PK754_02100"
     CDS             17092..18708
                     /locus_tag="PK754_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB08326.1"
                     /translation="MRIKLSLWVVGITLSVMIGCSSRTAVQPDIKNNTTASASTDDHG
                     HKTETLAEEKKGPSEPVITVVSVPKTVLSAEEKMELRAVGDSLYEKGDFEEALPVWVK
                     ILEHADDKPTIAAAHYVLGTLFYQRQEYFKAELEFKRAVESDSTLIDVYRELGFVYFV
                     KGDYDKARASFETMLNYLPGDSEAVHWVDYTIGTQAYEEGLKLFTDESYDKAIEKFQS
                     AIAYLEEDTSANYKIHYFIGKSYLEKLDYDNALKHLSKAVSLRPEMHEGYTEIGAIFF
                     SRSDYPKAIGFFQRALTYQPSSSKALNNLGYVYFTLGNQALAGGDKNRSDEFYKKAVT
                     YFEQALIADPAMAGARYNLDHVRKILSGARKATAYTLLQAALKTENHAERIRTYYKAI
                     EQDSTYDDAYVNLGVAMFYHGQADSGMIMTEKTIAINAYNAQAHNNLGYMLGTAHRWE
                     ESLKHIFLAIQIKRDYTDAYVNLGYVYMWKEDFESSRKVWLQLLKNNPDNTMARKGLA
                     EMERREKMVRSGETTTHIEINEDRAGGGSN"
     gene            18803..19813
                     /locus_tag="PK754_02105"
     CDS             18803..19813
                     /locus_tag="PK754_02105"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNB08327.1"
                     /translation="MSESKTIRALAVHAPGALLSSFEYGRTSLGADEIEVKVEYCALC
                     HSDLHLMDGDWACAFPFVPGHEIIGSIIQTGVSVRDLKPGQRVGIGWQCGSCGICEVC
                     ITGRAHLCEAGKKRTCVNQYGGFAEYVRAHQHFVYAIPETIPSIHAAPLMCAGLTVFS
                     ALQTWVTPKTRRIGIIGMGGLGHLAVQFAARMGCEVTAFDLSEDRLTLAKELGAHQAA
                     LTDSAPKGTFDLMIAATAASLDWNHWLRHLTWGGVLCLLGYPAEEIRISPDILLDGQK
                     IVTGSVVGSPETMRTMLDFAARHHVRPMVEELPMKQAHEAVARLRANKVRYRMVLKAD
                     WE"
     gene            19934..21010
                     /locus_tag="PK754_02110"
     CDS             19934..21010
                     /locus_tag="PK754_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNB08328.1"
                     /translation="MIYPVIMAGGSGTRFWPRSRAHRPKQLLNIFGEHTMIQHTVNRI
                     ASIAKPSEILVVTNKAQASEVHRQLPQLPSSNILVEPVGRNTAPCIGLAALHVMEKDP
                     NGVMVVLAADHLITPDDVFCKTVEAAAEVALRTEACVTIGIVPTRPETGYGYIQFIEN
                     ESLEAHGEKAYRVKTFAEKPNLETAKSFIATGDFLWNSGMFVWKASTILRLIEMHLPE
                     LYEGLVEIRPTIGTADYTATVDQVYRRIKGISIDYGVMEHTKEVYVLKGHFRWNDVGS
                     WEEVYQLSNKDENGNAVIGQAVCIEANNSLLFSPGKMIALVGVSDLIVVETEDALMIC
                     PRDRAQDVKKIVETLQAQKKNELL"
     gene            21018..21311
                     /locus_tag="PK754_02115"
     CDS             21018..21311
                     /locus_tag="PK754_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08329.1"
                     /translation="MKKEKILDTLEDIAAKLGLKVRHEKGDFAGGLCVKDDTQWILLN
                     KRHMIDRRINILARELAPFDLTGIEVPKPVMTIIESERKRATQDELELTDSEE"
     gene            21473..23341
                     /gene="uvrC"
                     /locus_tag="PK754_02120"
     CDS             21473..23341
                     /gene="uvrC"
                     /locus_tag="PK754_02120"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNB08330.1"
                     /translation="MNDTLEHKLEHLPKTPGCYLFKNGEGKIIYIGKAKILRNRVRQY
                     FQEGKRDDTKVAVMVSKVVDLEVIQTDSEIEALILESNLVKEHKPRYNIELKDDKSFP
                     YIKVTDEMFPRIYVTREKERGGGKFFGPYTDVKNLRHTLKTLHRIFPIRSCRHYFTPE
                     LVESKKIKLCLDYHIKKCDGPCQGLVTKEDYRAVVDQMTRFLNGKTGELIRQLKVAMQ
                     SYADAMKFEEAARLRDRIASIENYTAAQKVMFEDMADRDIAAVAIEADDACGVIFKIR
                     DGKLIGRQHYYFSHVTDKAPEEVLESLLQGYYMQADFVPRHIYLPQALSEETALTTWL
                     MQKSGERVELVVPKIGEKHKLVTMCERNAELLLGELKLQRLKAKEQFVPRVLESLKRD
                     LSLQRIPRRIECFDNSNIQGTDPVASMVVFVDGKPKKSDYRKFKIKTVEGPDDFASMN
                     EIITRRYLRVLEEGLDFPDLIVVDGGKGQLSAAVKALEALGIVVSRAGSEGQAIVGLA
                     KRLEEIFIPDVHDAIMIPKTSSAIKLLQHVRDEAHRFAITFHRSLRDKRTLVSEIDQI
                     EGVGEKRRTLLLQHFGSVQALSEAGLEEIMLVEGIPGDVARQIYAYFESRDDEESH"
     gene            23402..25243
                     /locus_tag="PK754_02125"
     CDS             23402..25243
                     /locus_tag="PK754_02125"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HNB08331.1"
                     /translation="MSEFLETLAKRVLVCDGAMGTVLYEKGISFDHCFDEINLSHPEM
                     VRAIHEAYIDAGADIIETNTFGGNRFRLMHHGLEEKIQAVNAAGVRIAVTTAKYKTEA
                     LGRRIFVAGSIGPLGKPLEPIGKITHAEARAYFAEQAKILADNGADLLMIETIGDLEE
                     AREAVTGARSVTGLPIVAQMTFNDEAKTLMGNKPSEVARVLKEAGANIVGANCSVGPQ
                     ALLEVLEKINIVPDIGFSVQPNAGLPRLVGGRYIYLASPDYFSDYAKLYMQAGANIIG
                     GCCGTTPEHIRQIKKAIGDQPPKKIEKKTAVIETEESSKAEQPPSHLRSSLIDKFRDK
                     KFVISVELDPPRGLNYDKVIEGAVMCRRHNVDAINIADNPLAKAGMTPLAMAGLIKQS
                     VQIETILHFSCRDRNLIAMQSELMSAHVMHIRTILGITGDPPLVGDYPNATGVFDVDS
                     IGLINLIRNLNNGIDLAGKSIGSRTNFFKSCAVNPTAVDMAREYDRYDQKIASGADFA
                     MTQVLYDLKPLEDFAKKYKGKIPVLLGIMPLKNAKHANFMHHEIPDITIPENIRERMT
                     KAGDRGQEEGVLIAKEFLREAKSMVDGVYIMPPFNKFEMAFDVLEVL"
     gene            25256..>26098
                     /locus_tag="PK754_02130"
     CDS             25256..>26098
                     /locus_tag="PK754_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HNB08332.1"
                     /translation="MKPTEIKTEIGRSEATHRQSSRGALWRNMLAAMRPLHWVKNIFV
                     LVPLLFSASEWTSVQIVYISAAFGLFCLAASAVYLFNDVVDREEDARHPSKQLRPITR
                     GLVSVHDALFMATILAFASIGGAYALAPALAYVIAGYVALNIGYTWFLKRWVIVDAVV
                     IALGFVIRIWAGAAAVNIPTGTWMLVTTFFLSALIAFSKRRYEIVYDGTEAFHTRGYT
                     PYLLDMLILLSAASVIGSYVFYILARGTWQHEYIILSTVLVVFYGTMRYIYVIHRNEE
                     RLDHT"
BASE COUNT         7070 a         5609 c         6335 g         7084 t
ORIGIN      
        1 attttttcaa gcgcgacata gtcttgtacg gtctgcagct cgtcctgcag cgtgacttca
       61 ggcgctgcgg cattttgcaa cgcgtatcga aggatattgg ctaacttggt aatcgcttct
      121 tgcgcacgtg caccgtcgct gccggctaac gcgcgaatgc tgttaagcgc attaaaaaga
      181 aaatgcggat tcatctgcga cttgagcgtc atcagttccg catctttaag agcggattca
      241 agtttcagat ttttgatttg tgaattttta cgatcctgaa aaaaatgaat gctaaagtaa
      301 atcacagacc aaagtaacgc cacgacgctg aagttgaaca tgcgcgctaa tgtgacggcg
      361 aatcgaaatt cacgaggtgc atcgggaacg aagtggctgc tcaaaaaaac accgcattcc
      421 atgatcgcgg cgaccagcag agttttgaat aaaataatcg gtataagccg taaaagagac
      481 cagctcagca tgtcatgccg acggatagcg cttcgaaaag cgtgggtacc gatcaggccc
      541 atcgcgctga cgaacacatt agccaaaaca agcggtaccg tgagatgata aaatcccgcc
      601 ataaaaaggc agttgatcgc ggcataaccc agccagccgc cgagctgaca taaccaatac
      661 atacgctggt gttgattcat accacaaatt ctttgtctat aaaatgtaag ctatattctt
      721 tactcgactc atcatcattg cgccgtagga ttgggagtac gttttttgaa aatcatacag
      781 cggcatgaaa gcataccata tccgatcagc ataccgatgg ctaatcccat agccagatca
      841 ttgagactgg cacccaaagc gataccgatg cctgcggata cggcaaagcc gggtcggtgt
      901 tttacgtttt gacaaaggcg cttgaacata cggactcctt tcaaataatt caaagagtgg
      961 ttttagtata gtgttaacgt gttttcaaaa acgacagcac ttcgctgaaa aaccagtttg
     1021 gttcatccag catgatgaag tgccgtgctg tcggtgccat gtgaatggta accgaaggct
     1081 gagcggcata gttttgttga aataaaccca gtaccatatc cgacgtcata ccgaaagtct
     1141 tataaccgcc ccatgaaccg agcaccagcg taggaacgcg gatcgcgctg atgtttttac
     1201 ggtaatcggt cgtcatcatt tcacgcatag cggcagtcaa gctcgtgcgg tccgatgcga
     1261 tgctccactc ggcgacggtg cgcgactgcg tcgaatcact gaccatcgtg cggatcgtgg
     1321 catattgtga ttgcgcgaaa ttgacggcat cctgattgag cagcatacgc tccatcatcg
     1381 cggcttgtgg tttggccaat tctaccgttg ctgccggatt ggccagtgca gggaggaaag
     1441 gtacggcatc cacgacgatc agctttccga tcacatccgg gtattctgat gcgatcatca
     1501 agcctaaaaa tccgccgagg ctgtgaccga tcacgatggg tttggttttg cagttttttc
     1561 gaatgtaagc cacgagcgca tcacgacgcg gagcgagaaa atcgcccgac acagccggca
     1621 caccggcaaa accggctaat gttacgacat ggcatgtatt ggttttttca atttgaccga
     1681 ccgttgtttt ccaaacttca ccggaacaac ccagtcccgg tatcaagatg atgtcaggac
     1741 cttgtccgta aacaataact tgaccaaagt ccgtccgggt ttgcgtatgc cccgaaacgg
     1801 tcaaaagtaa cgaggccagc accgctgcga taagccatga gatcgaatac aaacgcatac
     1861 atttctcctt gttttattaa cgaacaagag aaatctatac gaaaaaacga gagggggtta
     1921 gccaattggg atgaacggcg gatcaagggg atgagcggtc ggagataaag ggtaaacgtt
     1981 aaccgactct acataaacgg aaaaatgttt tatccgtatg tgcaaagtca ctataattat
     2041 catttaggct ttttgctagg ttgcgttgtc attaagattg cacggattta cttcttttga
     2101 gaaaaaaagc tgtaatgcta actgaaattt agggtactta aataactttt tgtataagat
     2161 catgctcgaa taagttagtt cgtcattaag agagggggca attcatgatc aatctcctac
     2221 tgaaatttac agattgatgt acagtttatg ggagttacga atgaaagctt ttccgaggta
     2281 ttcatgcagg taaaacccga ttttgaccaa atgcagcctt ggattggcaa atttggtttt
     2341 cccaatactt ttttttacaa tctaaggaac gtatcgtaaa atcaaacgta tcatatttga
     2401 acatatatgc cttttagaat gaaaaaaatg aactacatat tcgttttgtg gactacgttg
     2461 atctggggca tgttcgccgc actttttgtt ctccagtcct atttttttag cattgcgatc
     2521 ggccagtcgt ttgacttgtg gctccgccta tcgtttgaag gttcacaggc attgatatgg
     2581 gcgattttga caccggtagt tgttagtttc gctaagcgat ggaatcttca tggtgcgtcc
     2641 gccccagcct cattgtccgt acattttgta cttggatttg ttatcggtta tatacaccgc
     2701 gctgcgtcgt tttggctcta tatcgcactg tatccgccgg atacgcccct gccgtcgtcg
     2761 gttgcctggg ctaaaattct cggcagtact atgaatagtg ttctgatcta ctggtgtatc
     2821 gtcgccgtgg gatacgtcgt gcattacgtg cgtgcgcatc agcaagaacg attgcaagca
     2881 gcgcatctgg aaagtctttt tcagcaatcg cgtttggaat cgttgcgccg tcagatacaa
     2941 cctcattttt tgtttaatac gttgcattcg atttcggctc tgatggatga cgatgttccc
     3001 ggcgcgcggc gaatgatcac ccggttgagt gatctgttgc gcaaaacgct ggatcaatcg
     3061 gaacgttcaa aaataccgct cggcgaagag atacgattta tcgcggatta tctggcgatc
     3121 gaatccgtcc ggtttcagga tcgtctgcaa gtccgctatg aggtggctcc ggaagttgaa
     3181 aaagcattgg tcccttctct gattctccaa cctttggttg agaatgccat tcgtcatggc
     3241 gttgctcatg tggcatcacc gtcagtgatc attattcgcg ctcttcggaa tgaggaaaat
     3301 cttaatataa cgatcttaaa cacatcggta ccggtcaacg aattggaaaa ggggattgga
     3361 ctgaaaaata cggaatctcg cctgcagttg gcttacggcg agcgggcggc gttgaaaatt
     3421 ctgactatac aggatcaatt tagcgtaacg atagaaattc cctatgaaga cagagtttga
     3481 tcatgaatta atggcggtcg tcgcagatga tgaagtattg gcgcgaaagc gcattcgtca
     3541 tttgctggcg aaacgttcag acatccgtgt ggtagcggaa tgcgtccacg gttatgacgt
     3601 atgcgataag gttttggaat acaagccgga tttgttgttt ctggatattc agatgccgga
     3661 gatggacgga ttggaagtgt tgcgtacgct aggtccggtc gccgtgccgg gcgtggtttt
     3721 cgtaacggca tttgatcaac atgcgattcg tgcgtttgaa ctccatgctc tggattattt
     3781 gctcaaaccg tttgacgatg cgcgttttta tgaagcgctg gacagagcta tacaaatgat
     3841 cggaaaacaa caactaccgg ataacctcaa acggctgcta cagaatttgc cgcgtccgga
     3901 agcgtttatg ccgcgcatcc cggtgaaaga cgggatacga atacattttg ttgaaacggc
     3961 caatatactg tggatcgagg cgactggaaa aaacaccatg atgcatacca ccgacggttc
     4021 gatcaagact gcaaaaaaca taatcgaggt cgcccgggat cttgatccga cgcagtttta
     4081 tcgcattcat cgctcttata tagtcaatat ccgcgcgatc cgtgaaatgc agtcttggca
     4141 taagggtgaa tacgttgtga tactttcaga cgccacccga cttgtcactg gacgcgcttt
     4201 tcgtggcaat gttgatctgc tcatgcgccg ctgatattct tttcgttacc tgtaaaatcc
     4261 cttctattcc gcatttcgac cctttcgttc cttaattgca acgacttaat tctgtcatcg
     4321 taatcaattg gtatagacta gggtgcgata tacatcgcag gtaatatcaa accttgcaca
     4381 caaattttag aaattaatat ggatttcata aaacctaacc ccatcgttta atctaactga
     4441 aggagaagct atgaaaacgt ttatttggtt atggctaaca ataacttgca taggatcgac
     4501 gtcttgccgt gatcgatcgg ctggtgtgca agagccgaat gcactatcgc aagacgataa
     4561 agatgtacta tttcaaattc ataacggcga tttggcgggt gctgaatcaa aaatcaacgc
     4621 gatgctgagt aacaatgcgg ataacctgcg gggtcatttt ttaaaaactt cgttatactt
     4681 ccatgcgcgg tactttgcca gccagggcgc cagccgtgac tccatcacta cactcttgct
     4741 gaagtcctgc gaagaagcga taacacaggc tgaaaaacaa tcggagtcag ttgagcaaca
     4801 attttacctt ggttcatttt atagctttaa aagccgtgcg ctgcttgcca aacgagaatg
     4861 gacatcggct ttttttgctg cgcgaaccgg taaaaatttg ctggaatctg tgatcgaatc
     4921 cgcaccgggt tatgcggacg cttatttgcc tcttggtgtt ttatattatt tcgcttcaac
     4981 ccagcaggcg tggtggcaag gcgctttagc gtgggtagcg gatttggatg ttcccaaaac
     5041 gcaggcgctc acgtatatac aaaaaactta tgatgaagga ttgctcaata aaaatgaggc
     5101 gcattttgtc ttaaacaaca tcaatttatt ttttgaaaat aagttagata tagccgaatc
     5161 cctgttacaa aattataatg cccaatttcc caataaccgt ttttttttag cgccgatgca
     5221 aggtttaaaa ttaactcgta ttgcggaaga aaaaggaggg gagttcttac tcactcattt
     5281 agacagttta gtgacagcgc atcctctcaa tggtatgcaa acattgaatc gcgtgggtta
     5341 ccggtatttg gggcaaaaaa aattccctga tgcattggct gtctttcatg ctaatgtaaa
     5401 attgttccct gggtcggcca actgccatga tagtttggcc gaatgctact atacgatggg
     5461 gcaaatgcag gaatcaaaaa aatattacag tcttgccctt accaaactta gcggtgatca
     5521 aacaataaat gaggagttta aaaagacatt gaaagaaggt attgaagtca aactaataga
     5581 tttggcatca tattgaggtg agatgaaata ggtataaaat acaaaatccc gttccaaagg
     5641 cgggattttt tgtggacgcg actggactcg aaccagtgac ccctacgatg tcaacgtagt
     5701 actctaacca actgagctac gcgtccataa tacatttgtg ctcggaaccg gaatcgaacc
     5761 ggtacgggct ttgaggcccg caggatttta agtcctgtgc gtctaccagt tccgccatcc
     5821 gagctttaga cacacgctgg aggcgaagga cagaatcgaa ctgtcgatgg ggcttttgca
     5881 gagccctgcc ttaccacttg gctacttcgc catcttttca aagaccacaa aaaaataacc
     5941 agagccccga taggtcgaga atctggttac tcatttcact tactctaccg ttgtagtaat
     6001 cacaacggat cccatctgga ttacacgaac gagagcgaga gacgagattc gaactcgcga
     6061 catcaacctt ggcaaggttg cactctacca actgagctac tctcgcatat atatgaacaa
     6121 accaaatgcc taattcaaaa ctgggacagt aatatagata aaaatatttc ccatgtcaag
     6181 aaattttgaa taaaaacaaa aaatgatttt taagttaaat aaatacatat atttaagcgt
     6241 gtcatttttt taaattgaca cgctcatgta agtcaaaatg tcatgacata agtggcgaat
     6301 atgatgttgt aaacaagtta tcaacatgat tgatgttgtt tttattgaaa ctgaattggc
     6361 ttgcataacg tatcatgttt ttattaattt atcagccatt atgaattcta aaaaagcatt
     6421 aataattgta tttgtttggg ttttcgggct aagctcgtgt gcattattta ttgatccata
     6481 tgaggcccaa tatcgccagt tactccatga gcgggattca cttcagcttt cgatcgctcg
     6541 gactcggcat gatctcaaag atcttgaagc ggagctttgg aaaacgctgt atgatacgga
     6601 gtacttgcgt aacgcattgg ttttggctca ggccgattcc gtaaaaaatt tttactataa
     6661 ctacgtattt cgttatcgcc gtccgattca ggctattgcg gaaattgatc ccgcttcgcc
     6721 atttcttttt ccgcttaaaa ccgaaaaaga ccaaaaccgg attaaatttc gcatcatgtg
     6781 gggacgtttg ttctactttg actttaatca tcttggtatt gatatccgtg cactcgaagg
     6841 agatacggtt cgtgccatat atgacggcgt cattcaaaac tatggcggtg ccgagggata
     6901 tggcgaactt gtagccgtgg tcgaacatga atatcgcggt ctatggaata aagacgtagt
     6961 gccgcaacga ttcatgtcca tatacgggca tattcgaaat cgttgttatg atggcgagcc
     7021 ggaattgaaa tggaaagccg gagaccgcat ccgtaaaggc gatattatcg ggtttatcaa
     7081 tgatgacagt cgcaatgggt atggaatgga gcatttgcat ctcggtattc ggcgtcaaag
     7141 cgcgatggat gcgcaagcgg tggacggagg atattggatg cgcggatacg acacgcccaa
     7201 aaacagcaaa tttcagtttt ttatgaatcc gatagactta tttggtcgct atgttcagtt
     7261 tgttcttcct gaatagtcag atttctcttt gcattttata gataaattat ctattgttgc
     7321 caataagtaa ttttgcttgc cggttttttg tttttcatgg aggatttttc gaatgcgtct
     7381 gatcacgcgt accgatttgg atggccttac atgcgccgtc tttatcacga tcatggaaaa
     7441 aatagatgaa gttgtatttt acgaacccaa agaaatgcag gatggtaaag tacctgttac
     7501 cgatcgcgat atcatagcca atcttcctta tcaccctaat tgtgctttat ggtttgatca
     7561 tcatcaaagc cagcttattc atcaaaatgc agaatatcgt ggaaaatttg ccgtagcgcc
     7621 gagttgcgca cgggtgatct atgattttta cgaacagcct gacgaattaa aacccttcaa
     7681 agaattgctc gaggaaactg atcgcgtgga cagcgcacag ctgaccatgg aagatgtgat
     7741 gaatcccaaa ggttgggtgc tactctccta tacgctggac ccgcgctccg gccttgatgc
     7801 gtttacggac tattttcagc gtatgatcgg gtggatcatg gtgcatacgg ttgatgagct
     7861 tttgcaatta ccggatgtca aatctaaagt tgataaatat ttagctgatc aggataaatt
     7921 taaagaagcc ttgactacat attctcgcga agatggtaat gtcattatca cggatcaacg
     7981 ttccgtggat aaatttccgt ccggcaatcg gtttcttatt tatacgttat ttcccaaagc
     8041 caatgtatcg gtgcgcttat tcaaaggcaa agaacccggc gtcaccgtat gcgccgtcgg
     8101 gcacagcatt tttaatcata gctgtaaaac gatagtgggg gagcttatgc atgagtatgg
     8161 cggcggcgga cataagggtg cgggaacatg ccaatttcca gaagcagagg ccgaagcgaa
     8221 agttgcagag atcattgaac gattgaagaa aaacggataa tcggcttaaa attatataaa
     8281 aaggcaacct gaagaaggtt gccttttttt gtgtcaaatt ttgcgcccta agatttatgg
     8341 tgtggtttag tatcggtttg atacgttggt ttaattaaaa actaattgcc aacgaactaa
     8401 gaacggatag attagaagtt tcaccggaaa aaatagcatc acggctttgt aactggcgcc
     8461 cttctattcg caatacggat tgtttggtca aagcataatc cagattggct gatagcccca
     8521 atgtttggaa tccgtgtaca gtaccggttg aaacgatgac ctgattttta tccaagaatt
     8581 gctcggcgcg cacagcaacg ctccacatgt cattgagcat ataccgtgtt ataatagccc
     8641 atgtcagcca tgtgttgtat tttgaacttc ctttagattt ctgctccgca ccaaaatcaa
     8701 ctcctgtagt aatgcctaat tttgggttca cctgccaaat agcgtaggcg ttgtcgaaat
     8761 agcgccactg acgcgtactg tccgccttat cattacctac aaatgtgcta taattaatgg
     8821 ttaatcgatc tgaaggcttg tacacgatct gcgtgcccat tgccggagtt acgtttttag
     8881 aagaacggta aattcgttgc caaccgttac acaccacagc ggcaaaggtc catttttccg
     8941 ataaggaata tgttgccttg acaccgctaa gataatacgg cgaattttcg gcggccagtg
     9001 aacgcgataa tgtccaatta tcttttgaaa cagcgctttc ccaacctata tgtgatccaa
     9061 ataaaccgac ttcaagggag gtctttgcgt tgatttgtat accggtataa gcctcataaa
     9121 gatgctggta ggttgccggt tcagcggaaa gattggattg agcataagtt ccggccatga
     9181 tgccgacgcg tgcatacgct tcatcgtatt tagcctgcac tcgtaacaac gccagattta
     9241 cgttaaattc attgtgccgt gtatgattat aaagaaaccc gggtctatcg ccggagacag
     9301 gctttgaaaa gtcgtatccg tagtatgcgt ccacgaagcc cgaccatgac cattccggaa
     9361 tgttattctg agcccatgtc ggcagaacat ggatcgtaat catgaaacaa aataaagaaa
     9421 atcgcataac aaaacctggt tttttgtatg aaaaactatt ttttttccat cactgtttta
     9481 agattagcaa gaccggtagc caagtcatcg ccgatcattt tttccatgtc cataaagagc
     9541 aagaaaagat tcatcggata atccatgtgc ccgctaaagc cccacttgac cagcgtggtg
     9601 ctgtccgtag ctgcacttgt ggtcatataa gccggttctt tggattgcca cggttcaaaa
     9661 aaacgcaatt caaaatcaat gcgttcgcca tcagcgattt ttacgatttc ctgttcacct
     9721 ttaccgacat ccgggttttg actttcccat gccgacacaa atccaacctg cccgtcttca
     9781 ccgcgatagg tttttttcat gttggcatcc attgtagccc atttgctgta gtggtcctga
     9841 ttcttaagaa actttacgta atcaaataca acctgattgg gttgattgat cgtaatgcta
     9901 cgttcgacag catactgctt tttcacaaaa agcgctacca gaagcggcaa cgcgatgagg
     9961 atgcccaata cgatgagcaa cagacgaagt gctttcatga gaacctccgt gggtagggtt
    10021 ttagggtaaa gaatagtacg gttaaatacg gaatgtcgga tattaagtta cgtcaatatt
    10081 tgctgcgaaa gagccaaaac aaaacagaga gaatcacgct caacaggatg ccggacgtga
    10141 tcggaaaata aaacgagaag ttttcccgtt gaataaaaat atctccgggc aagcgcccga
    10201 tcagagggat tttatcggaa aaagtaagaa acattccgac ggcaatcaaa acaccgccga
    10261 ggatcatcaa tgtttttccg aaatcgtgaa atggctccat acgatcatcg tttaaaggtt
    10321 catcttttcg acgacgcgtt tcatgatcag tgtcattttg ggttcggctt caccggcggt
    10381 tttgatgatt ttgggcagat caaccggttg aagcgaatcg gggaaacact catcggtaat
    10441 aatcgaaaat ccgagacaag gaatacccat atgattagcg acgattactt cgggcacagt
    10501 agacataccg acaacatcgg cgccgatata acggaggaaa cgatactccg cacgggtttc
    10561 gagattcgga cccatcacgg cgacgtaaat gcctttttga actttaattt tttcttccaa
    10621 agcgatgttt tccgctaaag cgatcaatgt acgggaatag ggttcgctca tatcaggaaa
    10681 acgcggacca agcgtatctt cattttttcc ccgcaacgga ttatcaccga gcagattgat
    10741 atgatcatcc atgatcatga tatcaccggg ttggtattga ggattcatgc cgccgcaggc
    10801 attggatacg agaagcgctt tgatgccgag gaacttcatg acgcgaacgg ggaatgtgat
    10861 ctgttgcatg gtatagcctt cataaaaatg aaagcgtcct tgcatagcga cgacgtgttt
    10921 gccgccaagt gtgccgaaga tcagcttacc ggcatgactt tcgacggtgc tgagagggaa
    10981 atgcggtata tcctgatagg gaatttcgat ttctttgcgt atctcctgta ccaagccgcc
    11041 aagccccgtg ccgagtataa tcccgacaga cggctgtgtt ttggctttgg attgaatata
    11101 gttaaccgct tctgcgattt gtgctgtcaa aggactcatg atttctccga acgttttttc
    11161 tctaattctt tatgtatctg atccaacagt tcatgggatt ttttatccgc agaatcgccg
    11221 agcatttttt cgaaatccga ttttttaaaa atacccgtag cattgtgcgt aagcttttcg
    11281 agttcttcga tcactttctt tttcttttcg tggcgttccg ggtcagtaat cgccgtatcg
    11341 tcagccgatt cgcggatcgc cgcggctaac tccggaggca ggtcatccag tttgagatcc
    11401 tgtacaaacg aaccgatacc ttcttccagg ccggccttca gttcttcttc cgtggctacc
    11461 gggatgatgg gtggaggcag ttcggtttcc ttaatttcga cgggtttttc tacgcgcggc
    11521 tcgacctttt cgggaaccgg tattgtttta gcagcggctt cggccttttg atcacccacg
    11581 atttccaaaa gcgcaatttg agatttcagg tattcgcgca aatgcgcggc cactttggtt
    11641 ttttgcagct gaaggaaatt gacttcttga gcgagtgatt cattttcctt ctgcgcctgc
    11701 gtccggatgc gttcggcttc ggcgcgggcc tgttctaaaa tcaaatccgc atccacacgc
    11761 gcatccataa gtttttcgac tttgaccatt tcctgtttgt aatcattaag acttttttca
    11821 ctgctttgga tgcgaaggcg caagtactgg gtttcgtctt gaatcgcttt gagcttttgt
    11881 tgaagtaatt tagtctcgtc gagaatttct ttgtagtttt cagcaaccac ttcaagaaaa
    11941 gccgtcactt cgtcgggatc aaagccccgc atgacgcgtt tgaattcctg atgttgtatg
    12001 tcgccgggat tgagtttcac agcgtttcct ccgatttagg agcggccgat ttggcggcgc
    12061 gcttgcgttt tccgaaaagg gccgtaccga tacggatata gttggcgcct tcttcgatgg
    12121 ctatttcaaa atcactgctc atccccatgg agagcgcgtt catttccacg ttaggtatat
    12181 tttgttcgcg aataaaatcg cgcagttcgc gcaagcggcg aaagtaactg cgaatttttt
    12241 cgggatcatt ggcctgtaca gctgtcagag cgccgatcgt catgagtccc tggatgcgaa
    12301 tatttttcaa ttcagaaact tgtttgatca gctccagcgt gttatccggc tcgacaccat
    12361 atttggtggc ttcgcgggat gtattgacct ggatcaggat cggcatagtt ttatccaccg
    12421 tataagcgcg acgatcaatt tctttggcca aacgaaacga atctacggaa tgaatcatgt
    12481 caaacagttc tacggcttga cgggctttgt tggtttgcag gtgaccaatc aaatgcagtt
    12541 ggaagtccgg tacattgccg gactgttcaa gttctgtata tttctctttg gcttcttgaa
    12601 ccttattttc accgatgaga gaaatgccgg cagctacagc ttcgcggaca taatcaatgc
    12661 cgtatgtttt ggtaacggca ataagatgaa tttctttagg gtcacgtcca accttgcgtg
    12721 cggatgcttc tatttttttt cgaatcgttt caatattggc gcgaatcgtc gtttgcaatg
    12781 ctatatccat aaatcaagtt cctttaattt ctattttcgg ccaaaaccgg ctcgaatgcg
    12841 caattccggt tcggtttcga gtttaatacg ttcaatgccg cttatagttt tacctaaaaa
    12901 ctggtgcagc aaattatcac ccatataaag gaccgataag attatttttt cgtgtgcaac
    12961 ctcaatgccg atactggaaa atgtaatgcg tttgataaat ttagtcagat cgggattttt
    13021 ttctacggca tgcaggcgtt ccgccagctt cgcgtcaatg tgaaactttt gattgaatac
    13081 ctgatacgaa tcaccggtct tggtgaaacg gaattctctg tcggtaatcg cgtcagtcaa
    13141 tcggcggcta ccttcggaaa taactgaaag cacaccgctg ccgtcggtcg taaaaacttt
    13201 aggcgtgccc catttatcag ctaatatcaa tccgacgcaa ttataatctt cgatgagttt
    13261 tttaaaaaaa agctcaatcg caacatgggg aacggtactg cggtttttgc gcgcctcaaa
    13321 aaggcgcgcc acattgagta catcttcttt gaccttgcgc accgcgcagg cgggtttgag
    13381 atgaatacat ccttcgtgca ggcaagactc gaccggttcc acacagccga tacgctcttt
    13441 gtagtccaaa agattttcac agcgcgtacg gacggtgcgt tccgtcgtcg tcacaccgtg
    13501 tttttcctga taatacaata taccgtcttt tatcaaagca tcgaggagat tattcaactg
    13561 atgataatta aaacgatcca gcaattctct aaacgcatcc ggttgcagta acgacagatg
    13621 aatgacctga atttcttcgg aattaaatac ctgcgggtcc agcgcgagga tgcgctgcga
    13681 gcgaaaaacc gaatcgggcg tttccgtcat agcggaagct gccggagata tatgctctgc
    13741 tgttgacata gaaaaaacaa tacgattcat cgtttgaata caaaaacaaa gataggccgt
    13801 atatatctga aaatcaacca aaatgcctat ttgtatgctt gcgatgtgag aacgaacatt
    13861 catgcggtca ttgtaaaaca ggtatttaga cgtttcaggc gtttttttgg aagtatgaag
    13921 tgaatgttta agatgctatt ttgtatgcgg tgtgttcgga tttatatccg tggaataata
    13981 ccctgttgat gattaaaccc gtttttgtgt tgcgtgatgc gattgtattg attataattg
    14041 caaagtttta gtgttaaccg atggaagaaa aaactatgaa caaacgttgg atgattttcg
    14101 tcgtatggtg ttgtgcgggc gtactgtcag cgcaacaata ccaggttcgt atttcgccgg
    14161 atacgatttt gtgcagtgac caagaagcga tcatacgtgt atcaccgggt cgctccggtg
    14221 tcgagctcac ggccgttgcc cgcaaaggcg gtggttcgat cggtgatttt cgatatgcta
    14281 acagtgacca ggggtatgtc aatcaggcga ttttttcgag tcctttttcc ggggaaatcg
    14341 aaatcgaagt aatggataat aaaaacaaca aagtcggaat ggtaacagta accgtggttc
    14401 cgccgacgat ggaatttatg gaagacgaat ccatcgcgga atcgaatgcc gctgagaagc
    14461 gcattccgct gaagatcaaa gttttggatc agaacggtag ttttatcaaa acggctaaag
    14521 tgacttgtaa agtatcggaa gtggaaggca ccggaaaaaa taaaaagatc aaaccgagtg
    14581 atgctaaagt cggggcgttt gttttactgg acggatacta cgaaggtcag ttgaccaatc
    14641 ttaaaaatgc cacataccgt cttgaattct ttgatgaagc gcacttgctg ccttttgaca
    14701 gcgaagaaaa cccggatgca caacatccgt cgcttctgat tgattcatgg ccgatcagtt
    14761 tcaattgacc aacgaaaaat tatgatgaat acacggcccc gtgtatgtgg gaaaaacctg
    14821 caacacgggg tttgtttttt gtgaaaagag aaattactta ttttataatc cgattagccg
    14881 tttctattcc tcaattcgcc cataggatta aaattttgag tatgatcaaa ccgttttctt
    14941 tgagattaga cgaaccgcgc ggatggggtt ttgttttatt gttgttatgc ggctgtgcga
    15001 cgatcggtgc gcccggaggg gggccggaag ataaggaacc gccgacggtg ataacgacgt
    15061 ttcctgccaa agattcaacc ggagtatccc gtcagacgga ggtcacagtg actttctccg
    15121 aagcgatgaa taaagcttcg gtcgaaaaag cgttgtttat tgcaccgttc ccggcacgtg
    15181 atccggatct gtcgtgggat gatgcgacgt tgacgattcg ttttaaagac gcgctggaag
    15241 aaaaccgtac ctgcgtcatc acgatcggca cggacgctat ggatgcacat cagaataaat
    15301 ttaaaagcgc atggagtttt gctttttcga caggtgataa aatagacgcc ggttctatcg
    15361 ccggtacggt cgtatcggaa agcaaaacta aaccgagtgt atgggcgtac agactcaacg
    15421 gtcatacatc ctcacgcgac agcatgattt atactaaacg agccgactat ataacccaag
    15481 tggatgcaca ggggaaatat cgtttttcat atttggctga tggagcctac cgcatttatg
    15541 ccgtatccga tcaaggcgaa gattttaggt atcatccgat gcaggatatg atcggtcttg
    15601 cgccgcgcga tgtgatattg gcggatacat caaaagaagc gttgtttgtg gatatcgccg
    15661 tatttagaga agacaccacg accatggctg tacaattggc acaaatgacc gggcctaatg
    15721 aagttttgat tcgcatcaat aatgttttgg ctgaaagccg atatgacggt accaaaattg
    15781 gttcagaaca tattgcttcg catttttcaa ttcgcgatag cctgagcggt accgatgtag
    15841 caatacggga tgtatatttt gtaacgacgg gcgcaactga agaaattcga cttttatgcg
    15901 acagtatgtc ttctcaacgc gtgtatgttc tcgatgtgcg cggtatttat ggattgtccg
    15961 gcgactcgat tgcctttcaa caaattaatt ttcgcgcgga gggtggcttt gaaacattgc
    16021 gtgccaagat tgaatggact tttccatacg gtacgccggg gttgatcgca gttaatgatt
    16081 ttatcggatg gaaatccgac cgcggactgg atcgtccgac gtgggaaaag gcttttgtgt
    16141 tgcgtgatac tttaggtaac tcggtaaacg gaactttttt ttggatgcat tcggcatccg
    16201 gaaaattcat accggcgcgc aacctccggg ggcgtatgcc gtatgaggtg catgtggacg
    16261 gtaagatatt acaagattgg tccggtcgcc cggcgtttga cacgacgatg gtttggcgtt
    16321 tggtcgccat gccggaagac acggtcggca cggtatcggg tatgatcgcg gacgaatctt
    16381 ccgatgatcg cgggatgtac catataacgg cacgtaatct caatcgcagc gggcgcgatt
    16441 atacattgat cgtaccatcg ccggggggtt ttactttgga cgggatgctg cccggaaagt
    16501 ataattttct tgcattccgt gaccgggata gtaacggcgt attttcattc ggccaaacag
    16561 cgccgatcat ccgaccggag cgatttacaa gttatagcga tacggtgacg gttcgtaaaa
    16621 attgggaaac atctaatatt caaattcgtt tcaggaaata agacatgaaa agcatttatt
    16681 atttacatca gatcaaaacc ggaaaaaatt ttcgcgtgga agaaaatcag gaagtgacca
    16741 tcggtcgggc atttgacaat acgctcatga ttgacgataa ctccgtgtcg cgctatcatg
    16801 cgcagatcaa gtggaaaaaa aacctgatgt atattaccga tttacaaagt acgaacggaa
    16861 cttcggtgaa cggtgaaaaa gtggaacatg gttattttac agaacttaac ttctccgatt
    16921 tggtgaaaat cggcaatatt gaactgaaaa tccttgatga agaatcggtc atcggcaaaa
    16981 actttacccc tgatcaggca ccggccaaaa cgatcgtaat caatcaagcg atgttaaaaa
    17041 aagctgttga tcccgatgat tttacgcaca attaaaaata gagggcggta gatgagaata
    17101 aaactctcgt tatgggtggt aggtatcact ttgtcggtga tgatcggatg ttcatcgcgc
    17161 acggctgtac aaccggacat taaaaataat acgacggcgt ccgcttcgac agacgaccat
    17221 ggccataaaa cagaaactct tgcggaagaa aaaaagggac cttctgaacc tgtaatcacc
    17281 gttgtttctg ttccgaaaac cgtgctttct gccgaagaaa aaatggagct tcgtgctgtg
    17341 ggggattcgc tgtacgaaaa aggtgatttt gaagaagcgc tacccgtgtg ggttaaaata
    17401 ctggagcatg ccgacgacaa accgacgata gcggcggcgc actatgtgct tggtacattg
    17461 ttttatcagc ggcaggaata ttttaaagcc gagttagaat ttaaacgcgc ggtcgagtcg
    17521 gattcgactt tgatagacgt gtatcgtgag ttgggctttg tctattttgt caaaggtgat
    17581 tacgacaaag cacgggcctc atttgaaacg atgctaaatt atctgcccgg agattccgaa
    17641 gccgtacatt gggtagatta tacgatcggc acgcaggctt acgaagaagg tttgaaatta
    17701 tttaccgatg aatcgtacga taaagcgata gaaaaatttc aatccgcgat tgcttatctt
    17761 gaggaggaca catcggctaa ctataagatt cactacttta tcggtaaatc gtatttggaa
    17821 aagctggact acgataatgc attaaaacat ttgagcaaag cggtttcgtt gcgtccggaa
    17881 atgcacgaag ggtacaccga aatcggcgcg atcttttttt cccgcagtga ttatcccaaa
    17941 gcgatcggat ttttccaacg cgcattgacg tatcaaccat catccagcaa agcactgaat
    18001 aatcttggtt atgtgtattt tactctcggt aatcaggctt tggcgggcgg tgataaaaac
    18061 cgttcggatg agttttataa aaaagccgtc acctattttg aacaggctct gatcgctgat
    18121 ccggcgatgg cgggggcgcg atacaatctc gatcatgtac gtaaaatctt gagcggtgcg
    18181 cgcaaagcga cggcgtacac gctattgcaa gccgcattaa aaacggaaaa ccatgcggag
    18241 cgcattcgca catactataa agctatcgaa caagattcga cctacgatga tgcgtatgtc
    18301 aatctcggcg tagccatgtt ttatcatggt caggccgatt ccggtatgat tatgacagag
    18361 aagacgattg cgatcaacgc ctacaatgct caggcgcaca ataatctcgg ctatatgttg
    18421 ggtacagcac accgctggga agaaagtctg aaacacattt ttctggccat tcagatcaag
    18481 cgggattata ccgatgccta tgtcaacctc ggttatgttt acatgtggaa agaagatttc
    18541 gagtcgtccc gcaaagtctg gttgcaattg ctgaaaaata atcccgacaa tacgatggcg
    18601 cgtaaaggtt tggccgaaat ggaacgccgc gaaaaaatgg tgcgttccgg cgaaaccaca
    18661 acgcatattg agataaacga agatcgcgcg ggcggcggtt caaattaaaa tacatgtcgc
    18721 gacatggtat attcttgtta ataaatacaa atttatcgtt ttcttcttgg cctaattaac
    18781 gccgacatgc gtaccgatta aaatgtccga atctaaaaca atacgtgctt tagctgttca
    18841 cgcccccggt gcgctacttt cttcttttga atatggccga acgtctttag gcgccgatga
    18901 aatcgaagtc aaagtcgaat actgtgcttt gtgtcacagc gatttgcatc tgatggatgg
    18961 tgactgggca tgtgcttttc ctttcgtacc gggacatgaa atcatcggat ctattattca
    19021 aaccggggtt tccgttcgag atctgaagcc cggccagcgt gtaggtatcg gttggcaatg
    19081 cggttcctgc gggatatgcg aagtttgtat caccggtcgt gctcatttgt gtgaagccgg
    19141 aaaaaaacga acctgcgtta atcaatacgg tggatttgcc gaatacgtgc gagcgcatca
    19201 gcattttgtt tatgccattc cggaaacgat accgtcgatt catgccgcgc cacttatgtg
    19261 cgcggggctt acggttttta gcgccttgca aacgtgggtt actccgaaaa cacgacgcat
    19321 cgggattatc ggtatgggcg ggcttgggca tcttgccgtc cagtttgcgg cgcgtatggg
    19381 atgtgaagtc accgcgtttg atctgtctga ggaccgcctt accttagcga aagaattagg
    19441 cgcacatcag gcggctttaa cagactctgc accaaagggt acgtttgatc tgatgatcgc
    19501 agcaaccgcc gcttcgctgg attggaatca ttggttgcgg catttgacct ggggcggcgt
    19561 attgtgtttg ttaggttatc ctgcggagga aattcgcatt tctccggata tactgcttga
    19621 cggccagaag attgtaaccg gcagtgtggt tggaagccct gagaccatgc gaacgatgct
    19681 ggattttgcc gcacggcatc atgtacgtcc gatggttgaa gagctgccta tgaaacaggc
    19741 tcacgaggcc gtcgccagac tgcgagcaaa taaggtacgc tatcgtatgg ttctcaaagc
    19801 cgactgggaa tgatcaggac ttttgcttgc cattcacctc aaaccctctt attttcaccc
    19861 gcattttttt gaaacatgct accatcgatg aatctttggt aaacgatatc actttaacgt
    19921 gaatgaaaaa aatatgatat atcccgtgat tatggcgggc ggatcgggga cccgattttg
    19981 gcctcgcagc cgggcgcatc gtcccaagca actgctgaat attttcggcg aacatacgat
    20041 gatacaacat acggtaaacc gtatcgcttc catcgcaaaa ccgtctgaaa ttttggttgt
    20101 gaccaataag gcacaggcat ccgaagtgca tcgccaatta ccgcaattac cttcgtcgaa
    20161 tattctcgtg gaaccggtgg gtcgtaatac ggcaccctgt atcggccttg cggcgctcca
    20221 tgtgatggaa aaagatccga atggtgtgat ggtagtttta gctgcggatc atcttatcac
    20281 accggatgac gtattttgta aaaccgtcga agccgccgct gaagtagcgc ttcgcacgga
    20341 ggcgtgtgtg acgatcggga ttgtacctac gcgcccggaa accggctacg gttatattca
    20401 atttatagaa aacgaatcgc tcgaagccca cggtgaaaaa gcttatcggg tgaaaacctt
    20461 tgccgaaaaa ccgaatcttg aaacggcgaa aagctttatt gcgaccggtg attttttgtg
    20521 gaatagcgga atgtttgtat ggaaagcctc gacaatatta cgattgatcg aaatgcatct
    20581 tccggaactg tatgaaggcc ttgtagaaat cagaccgacg atcggtacag cggattatac
    20641 ggcaacagta gatcaggtat atcgtcgtat caaaggtata tctatagatt acggcgtgat
    20701 ggaacacact aaagaagttt atgttctcaa aggtcatttt cgctggaatg acgtcggaag
    20761 ctgggaagag gtgtatcagc tttcaaataa agatgaaaac ggtaatgcgg tcatcggtca
    20821 agccgtttgc atcgaagcta ataattcatt attattcagc ccgggaaaaa tgatcgcgct
    20881 tgtcggcgtt agtgatttga tcgtcgttga aaccgaagat gctttgatga tctgcccgcg
    20941 tgatcgtgcc caggatgtaa aaaagatagt agaaacacta caggcgcaga aaaaaaacga
    21001 acttttataa gtagcccatg aaaaaagaaa aaatactcga tacgttagaa gatatagcgg
    21061 cgaagctagg actgaaagta cggcatgaaa aaggcgattt tgccggaggc ctttgcgtca
    21121 aagatgatac gcagtggatt ttgcttaata aacgtcacat gattgatcgt cggatcaata
    21181 ttttagcgag agaattggct cctttcgatc ttacgggtat cgaagtgcct aagccggtta
    21241 tgaccatcat cgagagcgag cgcaaacgcg caacacagga tgaattggaa ttgacggatt
    21301 cggaagaata gccggaataa aatttttcat ttttatgaaa cctctttgtt taaacgaaga
    21361 ggtttttttg tccgttgatt caaagcggcg gtttttttat gtattcgatg agcgtactgt
    21421 gctggcgtag cgcctgtggc atatcgctcc aaaaaaatgt ataattgcag ccatgaatga
    21481 tacactggaa cataaactcg aacacttacc caaaacaccg ggatgttatc tttttaaaaa
    21541 cggtgagggt aagatcatat atatcggcaa ggccaaaatt ttaagaaatc gcgtgcgtca
    21601 gtactttcaa gaagggaaac gcgatgatac taaagtagcg gtcatggttt ccaaagtggt
    21661 tgatctcgag gtcattcaaa ccgattccga aatcgaagca ttgatcctcg aatccaattt
    21721 ggtcaaagaa cataagcccc gttacaatat cgaactcaaa gatgataaaa gttttcccta
    21781 catcaaagtt acggatgaga tgtttccccg catctatgta acacgcgaaa aagaacgcgg
    21841 cggaggaaaa tttttcggcc catatacaga tgtgaaaaat ttgcgacaca cgctcaaaac
    21901 actccatcgc atttttccga ttcgcagttg ccggcattat ttcactccgg aattggtgga
    21961 gtccaaaaag atcaaactgt gtctcgacta tcatatcaaa aaatgcgacg gaccgtgcca
    22021 agggttagtc acaaaagaag actatcgcgc tgtcgttgat caaatgacgc gtttccttaa
    22081 cggcaaaacc ggcgagttga ttcgccaact gaaagtcgcc atgcaatcgt atgccgacgc
    22141 gatgaagttt gaagaagcgg cgcgtttacg tgatcggatc gcatctatcg aaaattatac
    22201 ggccgcgcaa aaagtgatgt ttgaagatat ggccgaccgg gacatagcag cggtagcgat
    22261 cgaagcggat gatgcgtgcg gcgttatatt caaaattcga gacggaaaac tgatcgggcg
    22321 tcagcattat tatttcagtc acgtcacgga taaagcgccg gaagaagtgc tcgaatccct
    22381 gcttcaagga tattatatgc aggcggattt tgtaccccgg catatttatt taccgcaggc
    22441 gctatcggag gaaactgcac tgaccacatg gctaatgcaa aaatccgggg aacgcgtcga
    22501 attggtcgta cccaaaatcg gtgaaaaaca caaactcgta accatgtgcg agcgcaatgc
    22561 ggaacttctt ttaggcgagc tcaaacttca gcgtctcaaa gccaaagagc aatttgtacc
    22621 gcgcgtactc gagagcctca aacgcgacct gtcattacaa cgtattccgc ggcgtatcga
    22681 atgttttgat aactccaata ttcaaggcac ggacccggtg gcttccatgg tggtttttgt
    22741 agacggcaaa ccgaaaaaaa gtgattatcg aaaatttaaa atcaaaacgg tggaaggccc
    22801 ggatgatttt gccagtatga atgaaatcat cacgcgtcgt tatctgcgtg tactcgagga
    22861 aggtttggat ttccccgatc tgatcgtggt ggacggcgga aaagggcagt tatcggctgc
    22921 ggtaaaagca ctggaagcgc ttggcattgt cgtttcgcgc gcgggatcgg aggggcaggc
    22981 catcgtcggt ttggccaaac gcctggaaga aattttcata ccggatgtgc acgatgcgat
    23041 catgattccc aaaacgtcct cggccatcaa acttctacaa catgtgagag acgaagcgca
    23101 tcggtttgcc attacatttc accgcagttt gcgcgataag cgcaccttgg tatcggaaat
    23161 tgatcagatc gaaggtgtcg gagaaaagcg gcgcacgtta ttgctacagc actttggttc
    23221 cgttcaagcg ttatccgagg ccgggctgga ggaaatcatg ctggttgaag gcattcccgg
    23281 cgatgtggca cggcaaatct atgcgtattt cgaaagccgt gacgacgaag aaagtcattg
    23341 aagaaaggcc tctgacgctg tattatttat ataattaacc ttaatcggaa taaacacagg
    23401 tatgagtgaa tttttagaaa ctctcgctaa acgggtattg gtatgtgacg gagcgatggg
    23461 tacggttttg tatgaaaaag gaatttcatt tgatcactgt tttgatgaga tcaatttatc
    23521 tcatccggaa atggtacgtg ccatacatga ggcttatatt gatgccgggg cggacattat
    23581 cgaaactaac acgtttggcg gtaatcgttt tcgtctgatg catcacggct tagaagagaa
    23641 aattcaagct gtgaatgctg caggggttcg tattgccgtg acgacggcta aatataaaac
    23701 agaagcgttg ggtcggcgta tttttgtggc cggttcgata gggccgttgg gtaaaccgct
    23761 ggaaccgatc ggaaaaataa cgcatgccga agcgcgggct tattttgccg aacaagccaa
    23821 aattctagcc gataacggcg ccgatctttt gatgatcgaa acgatcggtg atcttgaaga
    23881 agcgcgcgaa gccgtcacag gggcacgtag tgtcacgggt cttcccatcg tcgcgcaaat
    23941 gacatttaac gatgaagcca aaacgctgat gggaaataaa ccatccgaag tggcgcgtgt
    24001 actcaaagaa gcgggcgcta atatcgtggg cgccaattgt tccgtcgggc cgcaggcgtt
    24061 actggaagtg ctggaaaaaa taaatatcgt tccggatatc ggattttccg ttcagcccaa
    24121 cgccggattg ccgcggctcg tcggcgggag atacatttat ttggcgtcac cggattattt
    24181 ttccgattac gccaaacttt atatgcaagc cggagccaat attattggcg gatgttgcgg
    24241 tacgacgccg gagcatattc gtcaaatcaa aaaagctatc ggcgatcagc cgccgaaaaa
    24301 aatcgaaaag aaaacggcgg tcatcgaaac ggaagaatcg tccaaagccg agcaacctcc
    24361 atcgcatcta cgttcctcgc tcattgataa atttcgcgat aaaaaatttg ttatcagtgt
    24421 ggaattggat ccgccgcggg gcctgaatta tgataaggtc atcgaaggtg cggtaatgtg
    24481 ccgccggcat aatgtggatg ccatcaatat tgccgacaat ccgttggcta aagccggaat
    24541 gacgcctttg gccatggccg ggttgatcaa acaaagcgtt cagatcgaaa cgatattaca
    24601 tttttcatgc cgtgaccgta atctgatcgc catgcagtcc gaactgatga gtgcgcacgt
    24661 catgcacatt cgcacgatac tcggcattac cggcgacccg ccgttggtcg gtgattatcc
    24721 caacgccaca ggggttttcg atgtggattc gatcgggcta attaatttga ttcgcaatct
    24781 gaataacggc attgatctcg ccgggaaatc catcggttcg cgtactaatt tttttaagtc
    24841 gtgcgcggtt aatccgacag ccgtagatat ggcgcgcgag tatgaccggt atgatcaaaa
    24901 aatcgcgtcg ggagcggact ttgcaatgac acaggttttg tatgatctca agccactgga
    24961 agattttgcc aaaaaataca aaggcaaaat cccggttctt ttgggtatca tgcctctaaa
    25021 aaatgccaaa catgcgaatt tcatgcatca tgaaattccg gatattacga ttcctgaaaa
    25081 tatccgggaa cgtatgacta aagccggcga ccgcgggcag gaggagggcg ttttgatagc
    25141 caaagaattt cttcgcgaag cgaagtctat ggtggacggc gtctacataa tgccgccatt
    25201 taataaattt gaaatggcgt tcgatgtgct ggaagttttg taaaatacta tatctatgaa
    25261 acccacagaa atcaaaacag agataggccg ctccgaagca acccatcgcc agtcatcccg
    25321 gggtgcattg tggcgtaata tgctggcggc catgcgcccg ttgcattggg taaaaaatat
    25381 ttttgttttg gtaccgctgc tgttctcggc ttcggaatgg acgtctgttc agatagtgta
    25441 catttcggca gcctttgggt tattttgttt ggcggccagt gccgtatatc tttttaatga
    25501 tgtggtggat cgggaagaag atgcgcgtca tccgtccaaa cagcttcggc ccataacgcg
    25561 cggcctggta tcggtgcatg atgcgctttt tatggcgacg atactggcgt tcgcttccat
    25621 cgggggagcg tatgcactcg caccggcgtt ggcctatgtc atagcgggat atgtggcgct
    25681 taatatcggt tacacctggt ttttgaaacg atgggtgatc gtggacgcgg tggtcatcgc
    25741 tttgggtttt gtgattcgta tttgggcggg cgctgccgct gtgaatatac ccaccgggac
    25801 gtggatgctg gttactacct tttttctgtc cgcgttaatc gcatttagta aacgccgtta
    25861 tgagatcgtc tatgacggta cggaagcgtt tcacacccgc ggttatacac cgtatttgtt
    25921 agacatgctg attttattat cggccgcgtc ggttatcggt tcatatgttt tttatatctt
    25981 ggcgcgggga acttggcaac acgaatatat tatactctct acggtcttgg tcgtgtttta
    26041 cggtacgatg cgttatatct acgtaattca ccgcaatgag gaacgcctcg atcatacc
//
LOCUS       DAOQNQ010000016        24989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_667_length_24989_cov_5.386059, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000016 DAOQNQ010000000
VERSION     DAOQNQ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 24989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24989
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_667_length_24989_cov_5.386059"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PK754_02135"
     CDS             complement(<1..716)
                     /locus_tag="PK754_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB08333.1"
                     /translation="MLHRYSVLTKLFLTLIGMLSLLLTAGFVVVSSGMALGWALLWCV
                     MAFALAAIIGYLLIRRITEPLYLMIGTLDSRSDFLSLSIPQVEQRRDEWGELYQHLHA
                     RLMLWKNLRTNMDERLHVMAKPLTDGDHAIRMQHDAMAEVQHALAEQITRHETNLRSS
                     VAAAKTYQELRIKLSDMAEAQKKIMRQAHTVYQQLFSSREQQSSRIERLGQLIKKFDD
                     VSDVVRRVHFINDQTKLIAF"
     gene            complement(706..1125)
                     /locus_tag="PK754_02140"
     CDS             complement(706..1125)
                     /locus_tag="PK754_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08334.1"
                     /translation="MKAGQHANTMDVLVFEIGMTLYGIPIGQIDMLDFVKSESQIPAG
                     NQIRHLLGFVSDRNPISQRSVKIKNPQPLRFIVEGIYGVFTLDVRRIADLPKTIGHQV
                     ERSPVSSAYISPKEQWDGRLVLLLDLQKKHITERYAA"
     gene            complement(1122..1637)
                     /locus_tag="PK754_02145"
     CDS             complement(1122..1637)
                     /locus_tag="PK754_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNB08335.1"
                     /translation="MDLKKTDNAELKLRSLGNTEEIAIDEEAIQYVVVTCGKTHFAFE
                     GIYVKEILPQTDITFVPGLPNFFSGMIHVRGNLECVADLGQLIGLQPTKTDKLNRVLI
                     VRYEEISSGILTEYVLDLIDVPKSKIRSDIKHTDAGRSDYFIGEFEWNNQIVVILDIV
                     RLMRKINGEEI"
     gene            complement(1615..2118)
                     /locus_tag="PK754_02150"
     CDS             complement(1615..2118)
                     /locus_tag="PK754_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08336.1"
                     /translation="MRSLYAKVLILGYGFWFWACGSSQPVPADAPFLHGLEVARWAET
                     ASVIRERVHSVAGWQWVTVLRDQNGLETTIVVRHVDEEYHLELDLNGRMQSISRIYPI
                     AQLDSAVSQLERMYREPDKVMQRPTYQMRRWEVYPEGYGVWIEMMITKEFFSIQAVYK
                     NGSQKNG"
     gene            complement(2121..3530)
                     /locus_tag="PK754_02155"
     CDS             complement(2121..3530)
                     /locus_tag="PK754_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNB08337.1"
                     /translation="MEELIKKIGAVADAEQIEAFVVGGYVRDRILGRKVTDFDVMVIG
                     DGVRFAEHVAKAFRLQTLVVYENFGTAMLPMGEKYDHLKVEFVGARKESYRHDSRKPL
                     VEVGDLQSDLSRRDFTINALAMRINAQDYGVILDLFNGRADLDKKTLRTPLDPDETFS
                     DDPLRMMRAVRFASQLQFTIDPITYDGICRNAQRIAIVSQERITDEFLKILGSPKPSV
                     GLYLLESTGLLAIIFPELTWLSGVEQQQGYLHKDVFKHTLKVVDNIGEMTNDLRLRFA
                     ALVHDIGKPRTKRFVEGIGWTFHGHEDVGSRMARGIGKKLKLPNDFWLYVSKLVKLHM
                     RPIQLVDEGVTDSAIRRLLFDSGADFDDLLTLCRADITSGNKERAKRHLENFQRVLDR
                     ARDVEEKDRLRAFQPPIRGDEIMQMFGLPPGPTVGALKKIIEEAILEGIIPNEYQAAY
                     DHLMAHKDEVLQSAENMQP"
     gene            complement(3531..3812)
                     /locus_tag="PK754_02160"
     CDS             complement(3531..3812)
                     /locus_tag="PK754_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08338.1"
                     /translation="MKTAFRQHVEQTVGARGIYSEREKRQLIEHVTQIHDALLAGEKT
                     DTIMENVEAFLDQFVNAFSTLEQKTDARNKKVQRREKKKSQLDIFKNEK"
     gene            complement(3809..4831)
                     /locus_tag="PK754_02165"
     CDS             complement(3809..4831)
                     /locus_tag="PK754_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNB08339.1"
                     /translation="MTAPQVNFSELQQLTDTDAIARLRQSRSAIEAEVAKAIVGQRAI
                     VEQLTIALFARGHCLLVGIPGLAKTLLINTIARALDLKFNRIQFTPDLMPSDIVGTEV
                     IEENMTTGSKQFRFVRGPIFANIVLADEINRTPPKTQSALLEAMQEHKVTASGNTYHL
                     EEPFFVLATQNPIEQEGTYPLPEAQLDRFMFNIWVDYPSADEEVAIVKNTTSSYHYAL
                     EKVLDAESILFYQNLIRRVPVADNVVRYAVDLVSKTRPKTPGAPKFISDWLSWGAGPR
                     ASQYLILGAKTRAALDGRFTPDIDDVRAVVKPVLRHRLITNFTAEADGVTTLDIIDKL
                     VAETTQ"
     gene            complement(4828..5217)
                     /locus_tag="PK754_02170"
     CDS             complement(4828..5217)
                     /locus_tag="PK754_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08340.1"
                     /translation="MFRTQGQYYWTDALYLARSKDQLRPVWREEKNTLYQCVHCAAFV
                     RFTEEQTGQWVRLKPYEQFQRRKEFLSFAPDAKQMKLIRQETRDRALFRCPVCASHWW
                     LEGDEWTHADDAIVEWAENSEFSKGTT"
     gene            complement(5387..7099)
                     /locus_tag="PK754_02175"
     CDS             complement(5387..7099)
                     /locus_tag="PK754_02175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HNB08341.1"
                     /translation="MIRSRFFHLLALFLLTQSTGWTQQKLNTAISDVTVYTDRAAITR
                     SGEIYLNPGEYQFVVSDLPHALQDQSLRVGGSGTAMARISDIKIELDYIDTMPQNRLK
                     DLQDQLALLMQDERVFNDRLGLLGKEKELLDQIKNSITQNKESNKLSFDDWSKLFGFY
                     DANYNKLNDEIRMLEKKRGDLTVRKNALQNQINQLSGYGKLVKKKVFLTVNVSKPGMM
                     NFAIDYVIGGAKWYPVYDVRVSPEDKSVDMTYYAMIAQRTGEDWSNVNLAISTARPNV
                     SGSMPQLSAWNLSVYQPIGILGRGGRSGAGMGAMSSAKMTKSAEEISDMSASSGAGEV
                     PEEAEAPIYEMETDEAAIETKSTSVVFKIKRKTNIPSDNYDHKVTITSESLKSDFAYS
                     SVPKLAELVYLRASIENTTEAPFLAGNANVFFGSNFVGTTYLNTVIPTEKFDVSLGID
                     DGIRIKRQQIRDYNAEKGIFNKSVKKTFEYKITIESFKRTDDTILVVDQFPQSQDERI
                     KVEAVLPEFPKEKWSSPHPNGVIEKTGGGLVQWRLRIKPKEKIELRIKYVVEYPRDVK
                     VDGL"
     gene            complement(7172..8008)
                     /gene="amrB"
                     /locus_tag="PK754_02180"
     CDS             complement(7172..8008)
                     /gene="amrB"
                     /locus_tag="PK754_02180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNB08342.1"
                     /translation="MSVTNKHVRRPAVAGLFYQADPAKLRDEVSGLLDNTTTQFSGGQ
                     PRGLIVPHAGYRYSGYVAAEAFATIRGMSYHTVLILSPSHHDYFHGISVIRESYRTPL
                     GDVPVALEAVDRILDRCALCKINILGHRDEHGIEVELPFLQVVLKNEFSIIPLVFGTQ
                     DNETIHALSEALTPFAGDPGVLLVASSDLSHFYNAVEGEKKDSIFLENVTAMDDDALY
                     TNIKTRKTEACGYGPVLAVMHAMKKNGITDSRILMYRHSGHVSGDFDEVVGYAAGLFY
                     KH"
     gene            complement(8023..9453)
                     /locus_tag="PK754_02185"
     CDS             complement(8023..9453)
                     /locus_tag="PK754_02185"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNB08343.1"
                     /translation="MSDSAVEKKKILVVDDDKNILRLIQAYLENDEYRIYNATDGKEC
                     FAMVDQDIPDVILLDLRIPGMDGIEVMKSLRIYYPKIPIIMMSAHGTIQSAVDAMQTG
                     AYDFVTKPFDSNRLKVSVRNALERKTKDDEIERLQKELYGPRDYGSIVGSSPAMRRIY
                     DLMDKIVASSNVTVLITGESGTGKELVAREIHKHDKSREKKPFVAFNCAAIPEHLLES
                     ELFGHEKGAFTGATERRIGKFEFAHGGTLFLDEIGEMSMATQAKLVRVIQEREVSRIG
                     GNDVIKIDVRLISATNKNLEEEVKNGRFREDLFYRLSVFPIVMPSLRERKEDVALLAT
                     HFMEKFKKREKKENISGMSREVLELLMGYHWPGNVRELENSMERAMVLASGNAITLED
                     LPPNIRTLSAPKPGYGKEMYMDMTGTMEEILERVEEKIIKKCFADCDGNISEVARRLN
                     IGRATIYRKAEKYGLPIKDYSTGESS"
     gene            complement(9464..10549)
                     /gene="lpxK"
                     /locus_tag="PK754_02190"
     CDS             complement(9464..10549)
                     /gene="lpxK"
                     /locus_tag="PK754_02190"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNB08344.1"
                     /translation="MIIKPPVQWKNSLLRYITYILFFPLSLLFAFLLLIKNSIAKLRP
                     VKNKVRVICIGSLSVGGSGKTPFVEWYARLLVARGLKLCVISNGYRKKSHGLVCVSDG
                     IKMNASLDASGDEAYMMAINFLNDNLNIPVISSPNRSHGIDFAEKNYDPDIILLDDGF
                     QDFSVYKDAVLCVEDYWERDYPQLWLPLGRLRDLKQNIKKCNAHVVTKIPPHAVLQTE
                     AGALPFLGFTYYRYAMYDLDDHPLNFEILKHKKIIVLAGLGHNDAFLRTVESIARDNS
                     AEVIHFAEFHDHHWYSRADIRRILSLQGSDTNDYIVLTSQKDAIKVKKEWVPSGFRFF
                     YLKPFMKPVHDAAADELAAIALRLKRQ"
     gene            complement(10539..11015)
                     /locus_tag="PK754_02195"
     CDS             complement(10539..11015)
                     /locus_tag="PK754_02195"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HNB08345.1"
                     /translation="MILFWHAHIFSVIAWLVVHNKSDQCVALVSASEDGQKLACVLKR
                     LGFTVVEGSSSRYGFEALLALRSLKYKNHWILITPDGPKGPARTCKPGFASLAQMHTR
                     YIHAVSLHTTNAWNLNSWDRAQIPKPFSKIGIQSTPIQLSVTSEIINTAERALHDH"
     gene            11197..11270
                     /locus_tag="PK754_02200"
     tRNA            11197..11270
                     /locus_tag="PK754_02200"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11231..11233,aa:Met,seq:cat)
     gene            11290..11363
                     /locus_tag="PK754_02205"
     tRNA            11290..11363
                     /locus_tag="PK754_02205"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11324..11326,aa:Met,seq:cat)
     gene            complement(11457..12365)
                     /locus_tag="PK754_02210"
     CDS             complement(11457..12365)
                     /locus_tag="PK754_02210"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNB08346.1"
                     /translation="MLIKPAATLKKIQDERRKKKAETNLLFASTFYQIVDFKCKCMAC
                     GLSKPELQQSFSISYIRNGYFEYNVYKNTFEAYTGRVLVDKPGYQYSVRHIRSISDEC
                     TIFDFTDEFYHQIGEKCGNRIAWYLKNKDRSSLLLDCNVELDYWHSLIRSKIRNSATA
                     RLEMDDLIIQLLTAVIGHMSSPATRKVLAEKNIKFHLLAIEKAKEFIAEKFTEDFSLL
                     DVARYCCVSPFHFSRIFKQITSYTPHQYLLSYRLQHAAMLINSTSLSLTEICYMSGFS
                     RSDYFSAAFSKKYKISPSQFRQANRV"
     gene            complement(12563..13069)
                     /locus_tag="PK754_02215"
     CDS             complement(12563..13069)
                     /locus_tag="PK754_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNB08347.1"
                     /translation="MRPGFFILVFFFLISSTYGVHSKTNYLSKSESINMNLTDEETDR
                     KILSALNAQFIKNYITNDTTSHSKLIHKDFVCIEGDGTILNREEYLKGWAHGYDPNIF
                     ISFTYQDELIRIFGNVALVRSKNVYSRMIQGKVVNGSTIYTDTYYKENGQWHCIQAQI
                     TAIKQTSK"
     gene            complement(13249..13902)
                     /locus_tag="PK754_02220"
     CDS             complement(13249..13902)
                     /locus_tag="PK754_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08348.1"
                     /translation="MQLKSRLTQAELLSTAIFTTDRAIFKQQDLIRNKIGELMSLMDD
                     EVRVFLTQIEGGFETRTDTMKIRLYGMLQEKLTFQILTLSVPDIQPERIRFFINDEPE
                     VLKLKSEFMMDQYNKLLNYAMILESAMRLIKNDRELIRRAFLITRDNTFLSIKGHPSR
                     SHRLPLTRQLLESDETAELKISKIALEIEEIEKLARHAKVISHEIDKITLEVSGETD"
     gene            complement(14138..15532)
                     /locus_tag="PK754_02225"
     CDS             complement(14138..15532)
                     /locus_tag="PK754_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNB08349.1"
                     /translation="MSPSKSLRILVIEDNATMREGIVDALMLHGLNAEAVSGGKEGLE
                     RMNRYPADLVITDYKMSPVDGMSVLKQVASQFPDTEVMMITAFGTVDVAVEAMKSGAS
                     DFITKPFSPDELIVKVDKILDGIVRRKHYEKTSEEVAYLRTEIDRVYNFSEIIGDAPS
                     MHGIFEIIRRSADSSSAVAIYGESGTGKELIARAIHYNSQRRDKPFIKVNCAALTETL
                     LESELFGHEKGAFTGAIKTKRGRFELADTGTLFLDEIGEISMNMQVKLLRAIQEQEFE
                     RVGGEDTLRVDVRIIAATNRNLQELIQRGVFREDLYYRLHVLPIVVPPLRERKGDIPR
                     LVDHVIRRVSENLGLANVSVEPGVYAALSSYDWPGNVRELENVIERAIVLSADRKLTA
                     ESFLGLTGKSAPFPIAYDDVEKSGLDEALASYEKTMIIQALNKAEGSKTEAARLLGVK
                     TSAFYYKLEKYGLH"
     gene            complement(15536..16396)
                     /locus_tag="PK754_02230"
     CDS             complement(15536..16396)
                     /locus_tag="PK754_02230"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HNB08350.1"
                     /translation="MIRWFVLFSMLVVASGSSAFSQKVTANVKIIRDKLQGRNLDLFE
                     GMEQKITSYVNNYDWSKTGDNTPMPVDIQIFLEKVSEVNELRRVHATMFFSNGKVMQS
                     LDKECIFSIRKGAVFYHDENVIDPFLSIIDFYIYIMLGDEMDSLGKLLGTPFFQKARS
                     LATQAKAFVPENVSGWDARLLKSDDFLDLRYQEYRIMKDHFYEAVSQLENEDAAGARQ
                     NATKALDILERIYTKNITSYHSERFIALNYLDFCKIFARSGEKKIYDRLIALDPKNKD
                     TYLKYRNEQN"
     gene            complement(16404..17195)
                     /gene="fadH"
                     /locus_tag="PK754_02235"
     CDS             complement(16404..17195)
                     /gene="fadH"
                     /locus_tag="PK754_02235"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013145906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-dienoyl-CoA reductase"
                     /protein_id="HNB08351.1"
                     /translation="MDGKVVLITGGGTGLGKSMAEHCAARGAHVIISGRRMDVLESAA
                     QDISRAGTRVLTVPCDVRVPDQVQAMTDKAVKEFGRVDHLINNAAGNFLCAAEKLSVN
                     GWNAVINIVLNGTFYCSSSVGKTMIQNGGGTITNIVATYAWASEPGVIHSASAKAGVL
                     AMTRTLAVEWARYGIRVNAIAPGAIRTEGTDKNLWGDPEQRSRMTGRIPMGRFGQPEE
                     VAHTLLFLLSDFSRYITGEVITVDGGAWIGKGTYEMIDVKNINQL"
     gene            complement(17227..17952)
                     /locus_tag="PK754_02240"
     CDS             complement(17227..17952)
                     /locus_tag="PK754_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNB08352.1"
                     /translation="MIAHLVTLQSIDTKLGEIEMLRGDLPKIVEQLKAELEELRDEIN
                     SDKQSVANFEKEKSDLTNEQSMNQEKLKKFQDQLYKATSNKEYEATSAQIDFCEQELN
                     RIKLRSIDIDAQVMELQEGVKPKEERLAGLEKDFSEREGELQTRIEETSKEENELRQK
                     REGLKPQIRPDLINKYERIRKAKNGIAVAPLVKEASCGGCFQQIPPQIIIELKKREKL
                     TNCEYCGRILYIESDVVKEASAS"
     gene            complement(18108..18905)
                     /locus_tag="PK754_02245"
     CDS             complement(18108..18905)
                     /locus_tag="PK754_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNB08353.1"
                     /translation="MSEKVLVQYEVKDRVAILTLTDPPANTYTHEMMRQLDECILKAR
                     FDENVNVIVLTGLGEKFFCAGANIDMLSKADPVWKYYFCLHANETLLRLEHTPKLVIA
                     ALNGHCVGGGLEIALAADIRIARKDAGMIGLPEVALGVLPGTGGTQRFARAVGRSLSI
                     ELMATGRKFAYEEALDMGLVNYIYEGNGASFMEQVMAYAKQFTIPNKAAMAVGHIKRS
                     VQTGIEVPLEQGLSLERELQSALFKSGDAKEGLMAFVEKRTPKFTGR"
     gene            complement(18928..19485)
                     /gene="folE"
                     /locus_tag="PK754_02250"
     CDS             complement(18928..19485)
                     /gene="folE"
                     /locus_tag="PK754_02250"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNB08354.1"
                     /translation="MKTQELITQLLRNYGENPDREGLRDTPDRVAKSFEFLMGGYQES
                     AEAVIQSAIFEENFNEMVLVRDIELYSLCEHHMLPFFGKCHIAYIPKGKIIGLSKLPR
                     VVDVFSRRLQVQERLTMQIANAIQDALHPEGVGVIIEAQHLCMMMRGVEKQHSLTTTS
                     CMLGVFKDDARTRTEFLTLAHSQNK"
     gene            complement(19686..20444)
                     /locus_tag="PK754_02255"
     CDS             complement(19686..20444)
                     /locus_tag="PK754_02255"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNB08355.1"
                     /translation="MNINKRILIGLGTVVLMVFGVMLYPRFAEYRLRTQLEKENEAAL
                     KEISKTDIIHAAAVADTMPVSFPEKAYLEVPFYCQAPHMNPDSWKIHKESCEEAAVLQ
                     AVFYNKGIKDVSVDSVDWLIKDMLRWQDQHFGGHKDIHADSVKMMMMGYFGYKDEEVK
                     ILRKAKIEDIKRYVAMGYPVIAPTFGRTLKNPFYTPPGPRYHMLTVIGYTPDRVITND
                     VGTRRGKDFTYPYDIFQKSMDEEGGDCLVIMAKK"
     gene            complement(20447..21205)
                     /locus_tag="PK754_02260"
     CDS             complement(20447..21205)
                     /locus_tag="PK754_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNB08356.1"
                     /translation="MKTLVIVVVCLFGILAGWGVSAQSPSSDIKNDGPKTSSVIASVV
                     QAMKDSLSKIPPEKALLDVPFFCQAPFMNMDSWNIHRESCEEAAALQVVYYLRGVKKV
                     DLSATDKILRDMIDWQMKQFKGHHDIRCDSTKTMIMSFFGYTDDEVRIIRKATIQDIK
                     DQILAGNPVIAMANGYVLNNPHYVENLRARGMSGYHMLTVKGFDSEKIITNDVGTMRG
                     NGYTYTYDIFQKAMDFHGGDIIVILPKDRTKTGQ"
     gene            complement(21261..22805)
                     /locus_tag="PK754_02265"
     CDS             complement(21261..22805)
                     /locus_tag="PK754_02265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNB08357.1"
                     /translation="MHRIVTPAEMQTIDRETIERHSIPGRELMARAGEVIFLTLTKRI
                     PDLASKRVLILCGKGNNGGDGYVLARCMISGGMTPKLIIAAEKKHIGGDALWHLEKLE
                     RTGVVVDASDAFEAPTEEYDVIVDALLGTGGRGHLSEPIKSWIEWINKQRFQKNAFIV
                     SVDIPSGLDGETGLFSDTAVTAHLTVTIGLPKRGLLFNDGKKYTGHLVIKDIGFPEVL
                     LSGGLWQFINQSDVAFHLPIRRHNSNKYDFGKLLIIAGSRGMSGAALMTARAAMRSGA
                     GVVRVAIPKSIAHVIEQGLPEAMTVHLPETEDGVISPAALSMIVDLHGWCTCAAIGPG
                     LSKNPAIAEWMFPLLSQWSVPSILDADALNILNGQWKVLRDVHSDMIITPHSGEFRRL
                     FGFEKNSATAMNDELHDWQKKLNHTILLKGVPTQIAGKSHVYVTHTGNPGMAKAGSGD
                     VLTGVIAGLMAQGLDSETAGFCGAYIHGLAGDIAVTDKTEYGLLAGDIIESIPQALQR
                     ITNCNS"
     gene            complement(23086..>24989)
                     /locus_tag="PK754_02270"
     CDS             complement(23086..>24989)
                     /locus_tag="PK754_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNB08358.1"
                     /translation="TVTIGSYGEFVVRGVIKKMDNKSHLTFDALGSLEFLKTWNGGSI
                     LAHEEWKNYYSTYTYVVVTPGTDAAMLQAGLDRIPEKVYRGITLETRDAGYAFKAQSL
                     QAITPGPMLSNSTSGGMPQAVLIFLMVLAGIGMFAALFNYANLTLARSLTRAREVGIR
                     KVSGATRAQLIFQFMGESVIITLVSFLVAETLLRVFLVPAFQSLSFTSELDIRFDPSA
                     GTYLLFAGFALCIGLLAGIIPAMVLSSFNPAVVIKDISKIRMFSGLTLRKTILISEFV
                     VTIILLIVLTTVYRQSEFASKMEYYGFDWRNTINVQLSGSQARVIADEMARFPGVVSY
                     SFASHAMGTWADHTVDVQTDPTKEASVVRDYSVDEHFIETMKITLLTGENLHRSESTG
                     DKVLVNEQFVKSLQLSNNSDAVGKVIYLDKSRPVTIAGVIKDFVFKPVTYAMEPVILT
                     YEPAQWSLVHFKITNENYKTVISFFKSVWKKFNPSIEFPYQVYSDELISVYSIFTDLG
                     RIIGLLSLLILTIAMLGLLGVASYSVETRTREIGIRKVLGASAMQLINLLSKQYLKLI
                     GIAMLIGLPVSLFLSNMILESFAYRTEFGIGIILPAVILLGFLVGLTILSQAWRGAHH
                     NPVTALRQE"
BASE COUNT         6817 a         6018 c         5045 g         7109 t
ORIGIN      
        1 ttaaatgcga taagtttagt ttgatcattg ataaaatgaa cgcggcgcac cacgtcgctg
       61 acatcatcaa attttttgat caactgacca aggcgttcta tgcgactgct ttgctgttcg
      121 cgcgaggaaa acaattgttg atacaccgtg tgggcttgac gcatgatctt tttctgcgct
      181 tcggccatgt ccgaaagttt gatacgtaat tcttggtacg ttttggcagc ggcgacggat
      241 gaccgcagat tggtttcatg gcgggttatt tgttccgcca aagcgtgttg cacctctgcc
      301 atcgcgtcat gttgcatccg gattgcatga tcaccgtccg taagaggttt agccatgaca
      361 tgcagccgtt catccatatt cgttcgcagg tttttccaca acatcagacg cgcatgcaaa
      421 tgttgataca attcccccca ttcatcgcga cgttgttcga cctggggtat tgataaagac
      481 aggaaatccg aacgtgagtc cagcgtacca atcattaaat agagcggctc agtaatacgt
      541 ctgatcaaca aatatccgat gatcgcggcc aaggcaaacg ccatgacaca ccacagcagc
      601 gcccagccca aagccatgcc gctgctcact acgacaaatc cggccgtaag caacaaactc
      661 aacatgccga tcaaggtcaa aaataatttt gtaagtacac tatacctatg cagcataacg
      721 ttccgtaata tgtttttttt gcagatcaag caataaaact aaccggccgt cccattgctc
      781 tttgggcgat atataggccg atgaaaccgg ggatcgctcc acctgatgcc cgatcgtttt
      841 gggtaaatcg gcgatacgac gcacatccaa tgtaaaaact ccgtaaatac cttcgacgat
      901 aaatcgcaat ggctgcgggt tttttatttt taccgaccgt tgcgaaatcg gattcctgtc
      961 ggacacaaac cccaacaaat gacgaatttg atttccagcc ggtatctgcg attccgattt
     1021 tacaaaatca agcatatcaa tttgcccgat cggaataccg tacagcgtca taccgatctc
     1081 gaaaaccaat acatccatag tgttggcgtg ttgaccggct ttcatatctc ctcaccgttt
     1141 atttttcgca tcaaccggac aatatcgagt atcacgacga tctggttatt ccactcaaat
     1201 tcgccgataa aataatcgga tcgcccggca tcggtatgct tgatatcgga tcgaattttg
     1261 ctttttggca catctatcag atccagaaca tattccgtca aaataccgga tgaaatctct
     1321 tcataacgta caatcagaac acgattcagt ttatcggttt ttgtcggctg aaggccgatc
     1381 aattggccta gatccgcaac acattccaga tttccccgca catggatcat gccggaaaaa
     1441 aaattgggca atccgggaac aaacgtaatg tccgtttgcg gtaaaatttc cttcacgtaa
     1501 ataccctcaa atgcaaaatg cgtttttccg catgtaacga ccacatactg gatcgcttcc
     1561 tcatcgatcg ctatttcttc ggtatttccg agactgcgta atttgagttc ggcattatcc
     1621 gtttttttga gatccatttt tatataccgc ttgtattgaa aaaaactctt tggttatcat
     1681 catttcgatc catacgccgt agccttccgg atacacttcc catcgccgca tctgatacgt
     1741 cggacgctgc attaccttgt caggctcacg gtacatccgc tccagttgcg aaaccgcact
     1801 gtcaagctga gctatcggat aaatacggct tatgctctgc atccgtccat tcaaatccaa
     1861 ctccagatga tactcctcat caacatgtcg gacaacgatg gtcgtttcaa gtccattttg
     1921 atcacgaagc accgtcaccc actgccatcc tgctacagaa tgcacacgtt cacgaataac
     1981 tgatgccgtt tctgcccatc gggcaacctc cagtccgtga aggaatggcg catcggccgg
     2041 aaccggttgg gatgaaccgc aagcccaaaa ccaaaaacca taaccgagaa taagaacctt
     2101 agcgtataac gatcgcatac ttacggctgc atattttctg ctgattgaag cacctcatct
     2161 ttgtgcgcca taaggtgatc gtacgccgct tgatattcgt taggtataat accttcgagt
     2221 atcgcttctt ctattatttt tttcaaagca cccactgtcg gtcccggtgg caaaccgaac
     2281 atctgcatga tctcatcccc gcgaatcggc ggctggaatg cacgcagacg atccttttct
     2341 tccacatcgc gcgcccgatc taatacgcgc tgaaaatttt ccaagtgacg tttggcacgt
     2401 tctttatttc cggatgtgat atccgcacgg cataacgtca acaaatcgtc aaagtcggcc
     2461 ccgctgtcaa aaagcaaccg ccgaatcgca ctatccgtaa cgccttcatc cacaagctga
     2521 ataggacgca tgtggagttt gactaactta gaaacgtaca accaaaaatc gttgggtagc
     2581 ttgagctttt ttccgatacc ccgcgccatg cgtgaaccca cgtcttcatg gccgtgaaac
     2641 gtccatccga taccttcgac aaaccgcttg gtacgcggct tgccgatatc atgcaccaac
     2701 gcggcaaatc gcaaccgtaa gtcatttgtc atttcgccaa tattatcaac aactttgagc
     2761 gtatgtttga aaacatcttt atgaagatac ccttgttgct gctccacacc ggaaagccat
     2821 gtcaattccg gaaaaataat cgccagtaaa cccgttgatt ccagcagata caatcccaca
     2881 gacggtttag ggctgccgag tattttgaga aactcatcgg tgatccgctc ttgactgacg
     2941 attgcgatac gctgcgcatt cctgcaaatc ccgtcatacg ttatcggatc tatggtaaac
     3001 tgcagttgcg atgcaaaccg caccgcacgc atcatgcgca gcgggtcgtc ggaaaaagtc
     3061 tcatccggat caagcggcgt acgcagggtt tttttatcca aatccgcacg accgttaaaa
     3121 agatccaaga tgacgccata atcttgcgca ttgatacgca tggccaatgc gttgatcgta
     3181 aaatcgcgcc gtgacaaatc cgattgcaaa tcgcccactt cgacgagcgg tttgcgcgaa
     3241 tcatgacggt aactttcttt acgcgcaccg acaaattcga ccttcaaatg atcgtatttt
     3301 tcgcccatcg gcaacatcgc cgtaccaaaa ttttcataca cgaccaatgt ttgcagtcga
     3361 aatgctttgg ctacgtgttc ggcaaaacga acaccgtccc cgatgaccat gacgtcaaaa
     3421 tccgtaactt tacggcccag tatccggtca cgaacatagc cgcctactac aaaggcttct
     3481 atttgttcgg catccgcaac ggcacctatt ttttttatga gttcttccac ttatttttcg
     3541 tttttaaaaa tatccaactg cgattttttc ttttcgcggc gttgtacttt tttattccgt
     3601 gcgtccgttt tttgttccaa tgtcgaaaag gcattcacaa actgatcaag aaatgcttcg
     3661 acgttttcca tgatggtatc ggttttttca ccggccaata acgcatcatg tatctgcgtg
     3721 acgtgttcga tgagctgacg cttttctctt tcggaataga taccgcgtgc cccgacggtc
     3781 tgttcgacat gttggcgaaa ggctgttttc attgcgtggt ttccgcaaca agtttatcaa
     3841 taatatcgag cgtcgtgacg ccgtccgctt ccgctgtaaa gttagtaatc aggcgatgcc
     3901 gcaatactgg cttgacgacc gcacgtacgt catcaatatc cggtgtgaat cgcccgtcca
     3961 acgcagcacg ggttttggcg cctaggatga gatactgcga tgcgcgcggg cctgcccccc
     4021 agctcagcca atcggatata aacttcggtg cacccggtgt tttagggcgg gtcttcgata
     4081 ccaaatccac ggcataacga acaacattgt ccgctacggg cacgcgacgg atcagattct
     4141 gataaaaaag aatgctctcg gcatccaaga ctttttccag agcgtagtga tacgaactgg
     4201 tggtattctt gacaatcgcc acttcttcgt ccgcgctcgg ataatctacc caaatattaa
     4261 acataaaacg atccagttgg gcttccggca aaggatatgt gccttcctgt tcgataggat
     4321 tttgtgtcgc taacacaaaa aacggttctt ccagatggta tgtattcccc gaagccgtca
     4381 ctttgtgttc ctgcatcgcc tccaaaaggg ccgactgcgt tttggggggc gtgcgattta
     4441 tttcgtccgc taaaacgata ttggcaaaga tcgggccgcg aacaaaacga aattgtttac
     4501 tgccggtcgt catattttct tcgatcactt ccgtgcccac gatatcgctc ggcatcagat
     4561 cgggcgtaaa ctgaatacgg ttgaatttta aatccaaagc ccgtgcaatc gtattgatca
     4621 gcaacgtctt ggcgagaccg ggaataccca cgagcagaca gtgtccgcgc gcaaacaaag
     4681 cgatggtaag ttgctctacg atagcgcgct gacctacaat ggctttggct acttcggctt
     4741 cgatcgcgct tcgggactgg cgcaaccgtg caatcgcatc ggtatcggta agttgttgca
     4801 gttcggaaaa atttacttgc ggcgctgtca tgtagttcct ttactaaatt ctgagttttc
     4861 tgcccattct acgatcgcat catcggcgtg cgtccattcg tccccttcca gccaccaatg
     4921 agacgcacaa acagggcacc gaaaaagagc ccgatcacgc gtttcctgac ggattagttt
     4981 catctgttta gcgtcgggtg caaacgataa aaattcttta cgacgctgga attgttcata
     5041 tggttttaga cgcacccatt ggccggtttg ttcttcagta aatcggacga aagcagcgca
     5101 atggacgcat tgatacaacg tgtttttttc ctcacgccac accggtcgta actgatcttt
     5161 ggaacgcgcc agataaagcg catccgtcca ataatattgg ccttgcgttc gaaacatact
     5221 ctccttccaa aactcttgta ttgtaataat ttagccaacg ataatcaata tgaataactg
     5281 gccacgtttt ttcaaattgt gacatgaaaa tacaaaaaaa cttcatgact ttatgtacgc
     5341 agtgtacaca aaatcatgaa gcttaacttg gtaatgaaga gattttctac aaaccgtcca
     5401 cctttacatc acgcggatac tcaacgacat acttgatacg caattcgatt ttttctttag
     5461 gtttgatccg aagtcgccat tgcactaaac cgccacctgt tttttcaata acgccattag
     5521 gatgcggtga tgaccatttt tcttttggaa attcaggtaa aacggcctct actttgatac
     5581 gttcgtcctg cgactgcgga aactggtcca caacaaggat cgtatcatcg gttcgtttga
     5641 aactctcgat tgtaatttta tattcgaaag tctttttaac cgatttatta aaaatacctt
     5701 tttcggcatt gtaatcacga atctgctggc gtttgatgcg aatcccgtcg tcaattccca
     5761 gcgaaacgtc aaatttttcc gtcggaataa ccgtattcaa gtacgttgta ccgacaaaat
     5821 tacttccgaa aaatacattg gcattaccgg cgagaaaggg cgcttccgtc gtattttcga
     5881 tacttgcacg cagatataca agttcggcga gcttgggaac agacgaatac gcaaaatcag
     5941 attttaagga ttcggaggta atggtgactt tgtgatcgta attatccgac gggatattgg
     6001 tcttgcgttt gattttaaaa acaaccgaag tgctttttgt ttcgatggct gcttcgtcgg
     6061 tttccatttc atagatgggc gcttctgcct cttcagggac ctctccggct ccgcttgaag
     6121 cactcatgtc tgaaatttcc tccgcagatt tagtcatctt ggccgaactc attgcaccca
     6181 tacctgcgcc tgaacgtccc ccgcgaccca atatgccgat gggttgatag acactgagat
     6241 tccaggctga aagctgcggc atagagccgg aaacattggg ccgcgctgta gaaattgcca
     6301 gatttacatt actccaatcc tcgccggttc tctgagcgat catcgcataa tacgtcatat
     6361 ctacggactt gtcctccggc gatacacgca catcatagac cggataccac tttgcgccac
     6421 cgattacata atctatggca aaattcatca tacccggttt actcacattg accgtcaaaa
     6481 aaactttttt ctttaccaat ttgccgtaac cggataattg attgatctga ttttgcagcg
     6541 catttttacg tacggtcaga tcaccgcgct ttttctcgag catgcgaatt tcatcattaa
     6601 gtttgttgta attggcatcg taaaatccaa atagtttgga ccaatcgtca aaactgagtt
     6661 tgttggattc tttattctgc gtgatgctat ttttgatctg atcaagcaat tctttttctt
     6721 taccgagcaa tccgagacgg tcgttaaaca cacgttcgtc ttgcattaac aatgcaagct
     6781 gatcctgcag atctttgagg cgattttgag gcattgtgtc tatataatcg agttcgattt
     6841 tgatgtcgga tatccgcgcc atggccgtac cactgcctcc gacacgcaac gactgatctt
     6901 gcagcgcatg gggcaaatcc gaaacgacga attgatactc gcccggattg agataaattt
     6961 ccccgctgcg cgtgattgcc gcacggtcgg tatataccgt aacatcgctg attgcggtgt
     7021 tcagtttttg ttgagtccat cctgtagact gcgtcagcag aaacagagcc aataaatgga
     7081 agaaacggct acggatcatg ataataactc ctcgttattt tataaataaa cctacgttgt
     7141 attcttaacg attgtcgcgc catcatcagc cttaatgctt ataaaacaac ccggcggcat
     7201 agcctacgac ttcgtcgaaa tccccgctta catgaccgga atgcctatac ataagaatgc
     7261 gactatcggt aatgccgttt tttttcatag catgcatcac tgcgagtacc ggcccgtaac
     7321 cacaggcctc tgtttttcgg gttttgatat tggtgtacaa tgcatcatcg tccatggcgg
     7381 tgacattttc taaaaaaata gaatcctttt tttcgccctc cacggcgtta taaaaatgcg
     7441 acaaatcgct gctggcgacc aataaaacgc cgggatcgcc tgcaaacgga gttaatgctt
     7501 cgctcaaagc atgaatggtt tcgttgtctt gtgttccgaa taccagcggt atgatcgaaa
     7561 attcgttttt taaaacaacc tgcaaaaaag gtaattccac ttcgatgccg tgttcgtcgc
     7621 gatgcccgag tatatttatt ttacagagag cacacctatc taagatacga tccaccgctt
     7681 ctagtgcgac gggtacatct cccaaagggg tacgataact ttcccgaata acagaaatgc
     7741 catggaaata atcatgatga ctgggactga gaataagcac cgtatggtac gacatgccgc
     7801 ggatagtcgc gaacgcttcc gccgcaacgt aaccgctata acgataaccg gcgtgcggta
     7861 cgataagtcc gcgcggttgc ccgcctgaaa attgggttgt agtattatct aataaaccgg
     7921 atacttcgtc gcgcagcttg gcaggatcgg cctggtaaaa aagccctgct acagcgggtc
     7981 ggcgcacatg tttattcgtt accgacataa tagaagtatg tgttatgaac tttcgcctgt
     8041 agaataatcc ttgatcggca atccgtattt ttcagcttta cgatatatcg tagcacggcc
     8101 gatattgaga cgtcttgcta cttcagaaat attcccatcg caatcggcaa aacatttttt
     8161 gatgattttc tcttcgacgc gttctaagat ttcttccatc gtaccggtca tatccatgta
     8221 catttcttta ccataacccg gtttaggcgc gctgagcgtt cggatattgg gtggcagatc
     8281 ttccagcgtg atcgcgttac ccgacgccaa taccatcgca cgttccatgg agttttcgag
     8341 ttcacggaca ttccccggcc aatgataccc catcaaaagc tccaaaactt cgcgtgacat
     8401 accggagata ttttctttct tctcgcgttt cttaaatttt tccatgaagt gtgttgccaa
     8461 cagtgccacg tcttctttgc gctcacgaag gctcggcatc acgatcggaa aaacactgag
     8521 acggtaaaac aaatcttcgc ggaagcgacc gttttttact tcttcttcga ggtttttatt
     8581 ggtcgccgaa atcagacgta catctatttt gatcacatcg ttgccgccaa tgcgagaaac
     8641 ttcacgttcc tgaatcacac gcaccagttt ggcttgcgtc gccatactca tctcgccgat
     8701 ctcatcgaga aataaagtac cgccgtgtgc aaattcgaat ttaccgatac gccgctctgt
     8761 tgctcccgta aacgcgcctt tttcgtgccc aaaaagttcg ctttccagca gatgctccgg
     8821 tatcgccgca cagttaaatg cgacaaatgg ttttttctcg cgcgatttat cgtgcttgtg
     8881 tatttcacgc gcaactaatt ctttgccggt accgctttca ccggtgataa gtaccgtcac
     8941 gttactgctg gcaacgatct tatccatcag atcgtaaatg cgtcgcattg cgggactgct
     9001 gccgacgatc gaaccgtagt cgcgaggtcc gtagagttct ttttgcagtc gttcgatttc
     9061 atcatccttg gtctttcgtt caagggcatt acgcaccgac actttcaggc gattggaatc
     9121 gaacggcttg gtaacaaaat catacgcgcc ggtttgcatc gcgtccacag ccgactggat
     9181 cgtaccatgc gcgctcatca tgatgatcgg tattttagga taataaatac gcaggctttt
     9241 catcacttcg atgccgtcca taccgggaat acgcaaatca agcaaaatca catcgggtat
     9301 atcctgatcc accatggcaa aacactcttt gccgtccgtg gcattgtaaa tgcgatactc
     9361 gtcattttcc aaataagcct gaatcaatcg aaggatattc ttatcatcat ccacgacaag
     9421 aattttcttc ttttcgacgg ccgaatcact catagtaaga acctcattgg cgttttaatc
     9481 gcaaagcgat ggcggcaagt tcatctgccg cagcatcatg tactggtttc ataaacggtt
     9541 ttaagtaaaa aaaacgaaaa ccggacggca cccattcttt ttttactttg atcgcgtcct
     9601 tttgactggt caggacaata tagtcattcg tatcggaacc ttgcaaagat aaaatgcgtc
     9661 gaatatctgc ccgtgaatac caatgatgat catgaaattc cgcaaaatgt atgacttccg
     9721 cagaattatc acgagcaata gactcaactg tcctcaaaaa cgcgtcatta tgaccaagcc
     9781 cggccaatac gattattttt ttgtgcttca aaatttcaaa gttcaaaggg tgatcatcaa
     9841 gatcatacat cgcataccgg taataggtaa atcccaaaaa aggcaatgcg cccgcctccg
     9901 tttgcaatac cgcatgaggc ggtattttag taacgacatg cgcattgcat tttttaatat
     9961 tttgtttaag atcgcgcaat cggcccaacg gaagccatag ttgtgggtag tccctttccc
    10021 aataatcctc cacacatagt acggcatctt tgtaaacgga aaaatcctga aacccatcat
    10081 ctaacaaaat aatgtcaggg tcataatttt tttcggcaaa atcaattcca tgtgaacgat
    10141 tgggcgacga aataacagga atattgagat tatcattcaa aaaattaatt gccatcatat
    10201 acgcctcatc gcccgatgca tcaagtgaag cgttcatttt tatcccgtca cttacgcaga
    10261 caaggccgtg cgattttttt cgataaccat tggaaataac gcacagtttc agaccacggg
    10321 caactaagag cctcgcatac cattcgacaa acggcgtttt tccgcttccc ccgaccgata
    10381 aacttccgat gcaaatcacg cgaactttgt ttttaacggg tcgaagttta gcaatcgaat
    10441 tttttatcaa taatagaaaa gcgaacaata acgaaagagg aaaaaaaaga atatatgtta
    10501 tataccggag taaactgttc ttccactgaa ccggtggttt aatgatcatg cagtgcgcgc
    10561 tccgcggtgt taataatttc agacgtaacg gacaactgaa tcggcgtact ttgtatgcca
    10621 atttttgaaa aaggtttggg aatttgcgca cgatcccatg aattcaggtt ccaggcattc
    10681 gtcgtatgca acgatacggc gtgtatatat cgtgtatgca tttgcgcgag agaagcaaag
    10741 cctggtttac atgttcgggc cggtcctttg ggaccgtccg gagttattaa aatccaatga
    10801 tttttgtatt ttaaagagcg taaagcgagc aatgcttcaa aaccgtaacg cgaactcgac
    10861 ccttcgacca cagtgaatcc caaacgtttt agcacgcatg caagtttttg accgtcttcg
    10921 ctagcactga ccaaggcaac acactgatca gatttattat gtacgaccaa ccaggcgata
    10981 accgaaaaaa tatgggcatg ccaaaatagt atcacaccgg gttcagctaa aatcgcaccg
    11041 tgcggtgtct taaatcttaa agtccgaaac catatgcgta ataacccggt tactactcca
    11101 caaactgttt tacgaaacat gaataatttt aataacccga tttattgctt gacattaaga
    11161 tctttaatct ctattattgg cgcacttaaa tgtgatcgcg gggtggagca gcctggtagc
    11221 tcgtcgggct cataacccga aggtcgtagg ttcaaatcct gcccccgcta ctaaaatatc
    11281 ttactacccg gtggggtagc tcagttggtt agagcacagg attcataacc ctgaggtcac
    11341 gggttcaact cccgtcccca ctaccccaaa aacccctcag caatgagggg atttttcgtt
    11401 ttaataatat tataaacggc acttcttcct ttgttttgca attcttgtgt ggtaacctat
    11461 acacgatttg cctgcctaaa ttgagatggt gaaatcttat attttttaga aaacgcggct
    11521 gaaaaataat cacttcggct aaatccggac atatagcata tttcagtcaa cgacaacgaa
    11581 gttgaattaa taagcatagc cgcatgttgt agcctgtagc taagtaaata ctgatgcgga
    11641 gtgtacgaag ttatttgttt aaatatccta ctgaaatgaa aagggctaac acaacaatat
    11701 cgcgcaacgt ccaatagcga aaaatcttca gtgaatttct cagcgataaa ttcttttgct
    11761 ttttcaatgg cgagtagatg aaatttgata tttttttcgg caaggacttt ccgtgttgca
    11821 ggacttgaca tatgcccgat tacggccgtc aaaagttgga tgattaaatc atccatttca
    11881 agtcgtgcag tagcagagtt ccgaatttta ctccggatta acgaatgcca ataatcaagc
    11941 tctacgttgc aatctaacaa tagtgaagaa cgatctttgt tttttaaata ccaagctatt
    12001 ctgttgccgc atttctctcc gatttggtga tagaattcat ctgtgaaatc gaaaatagta
    12061 cattcatccg aaatggagcg aatatgtctt acggaatatt gatagcccgg tttatcaact
    12121 aaaacacgtc cggtatacgc ttcaaacgtg tttttgtaca cattgtattc aaagtagccg
    12181 ttacgtatat aactaattga gaaactttgt tggagttcag gcttggaaag tccgcacgcc
    12241 atacatttac atttgaaatc tacgatttga taaaacgtgg atgcaaataa aaggtttgtc
    12301 tcggcttttt ttttccgccg ttcatcctga atttttttta acgttgccgc cggtttaatt
    12361 aacatagtag tattaatata aaatgatcag gcatttcatc aacgatatat ttcgactaac
    12421 cacacaagac ctttgcaagc aaaacaaact gggaaatatc tctgaaacct tagattaaaa
    12481 ttatacaaat aaaaaagccg aggctgcaaa atggaaaccc acatgcgcct cagctatata
    12541 caacaacgcg tcaggaatta atttatttag atgtttgttt gattgccgtg atttgggcct
    12601 gaatacaatg ccactgtccg ttttccttat aatatgtatc cgtatatatc gtgctcccgt
    12661 ttacaacctt cccttgtatc attctcgaat atacattttt cgaacgaaca agcgctacat
    12721 ttccaaatat tcttattaac tcatcttggt aggtaaacga tataaatata ttcggatcat
    12781 atccgtgcgc ccagcccttt aagtattctt cgcgattaag aattgtgcca tccccttcaa
    12841 tgcatacgaa gtctttatgt attagtttac tgtgtgacgt agtgtcatta gttatatagt
    12901 tttttataaa ctgtgcgttt agagcactca gaatctttct gtccgtttct tcgtcagtta
    12961 aattcatatt aatgctttct gattttgaaa gataattcgt tttagaatgt accccatacg
    13021 ttgaagaaat taaaaaaaag aaaaccaaga taaagaagcc gggacgcata attatactcc
    13081 ttgattcgtt taataattaa ataaccttta attattctac aacatcctta gaagatcagc
    13141 tttagaaatc ttgcttttct cattatttga atagaaagcg cggaaggtag tttttttaac
    13201 gggctacaat aaagacaatt cttacttatt ctttcgtagt gcgtagagtt aatcggtttc
    13261 tccactaact tctaaagtta ttttatcgat ctcatgagaa ataactttgg catgtcttgc
    13321 aagtttttca atttcttcaa tttctaatgc aattttactg atcttgagtt cagctgtttc
    13381 atcactttcc aataactgtc gcgtcaatgg cagcctatgg ctccgggatg gatgaccttt
    13441 tatggatagg aacgtattat ctcgtgtgat cagaaatgcg cgccgtataa gttcgcgatc
    13501 atttttaatt aatcgcatgg cggattctaa tatcattgca tagtttaata atttattata
    13561 ttgatccatc ataaattcgc ttttcaattt taaaacctca ggttcatcat taataaaaaa
    13621 acgaattctt tcaggttgaa tatcaggaac cgacaatgtt agaatttgaa atgttaattt
    13681 ttcttgcaac ataccataga gacgaatttt cattgtatcg gtacgcgttt caaaaccgcc
    13741 ttcaatctgc gtaagaaaaa cgcgcacttc atcatccatc agggacatga gttcaccgat
    13801 cttatttcgg ataagatcct gctgtttgaa aattgctcgg tctgttgtaa atattgcagt
    13861 agaaagcagc tccgcctggg taaggcggct tttaagctgc atgcggtcga atatatcaat
    13921 tgtcgggtgt tgtttgatgc ctaaaatttc acgaccgaga ctatcggcct cggattctaa
    13981 aatttcacgt tggatatgaa gactttgaat aatcgttgaa gcttgaataa tatcggtttc
    14041 aatccggcga ataagcgatg agctatacgc agactgcaga ctgtcttgcg ccgatagagc
    14101 cgtaacgcat gaccaaagca aaaaaagaat tactttgtta atgaaggcca tatttttcaa
    14161 gcttataata aaaggcgctt gttttgacac cgagcaacct tgcggcttcc gttttacttc
    14221 cttcggcttt atttaatgcc tgaattatca tggttttttc atacgacgcc aacgcctcat
    14281 ccaaacctga tttttccaca tcgtcatagg ctatgggaaa tggagcggat ttaccggtca
    14341 atcctaaaaa ggattccgcc gtcagttttc gatcggccga tagcacaata gcacgttcaa
    14401 tcacgttttc caattcgcgt acatttcccg gccaatcgta actgcttaaa gccgcataga
    14461 caccgggttc cacggaaaca tttgctaaac cgagattctc tgatacacgt cggatcacat
    14521 gatctaccag gcgcggtata tccccttttc gctcccgtaa cggaggtacg actatcggta
    14581 gcacatgcaa ccgataatat agatcttcgc gaaatactcc acgctgaatc aattcctgca
    14641 aattacgatt tgttgccgcg ataatacgga catccacgcg caatgtatct tcccccccta
    14701 cgcgttcaaa ttcctgttcc tgaatcgcac gaagaagttt gacctgcata ttcatcgaaa
    14761 tttcgccgat ttcgtcaagg aaaagtgtcc cggtatccgc taattcaaat cgtccgcgtt
    14821 tggtcttaat ggcaccggtg aaagcgcctt tttcgtggcc aaacaactca ctttctaaaa
    14881 gtgtctcggt tagcgcggca caattaactt taataaaagg tttatcacga cgctgactat
    14941 tgtaatgaat ggcgcgcgcg atcaattctt ttccggtacc gctttctcca tatatcgcta
    15001 cggcgctgga tgaatcagca ctgcgacgga ttatctcaaa aatgccatgc atgctcgggg
    15061 catccccgat aatttcagaa aaattgtaaa cacgatctat ttccgtacga agatatgcga
    15121 cttcttcgct cgttttttca taatgtttac gacgtacgat cccatctaaa attttatcaa
    15181 cttttacaat cagttcgtca ggactgaaag gtttggtgat aaaatcagag gctccggatt
    15241 tcatcgcctc cacagcgacg tccactgtac caaaagctgt aatcatcata acttcggtat
    15301 cgggaaactg tgacgcgacc tgtttgagaa cggacatgcc gtccaccggt gacattttgt
    15361 aatctgtgat caccaggtcg gccgggtagc gattcatgcg ttccaaccct tcttttccac
    15421 cggatacggc ctctgcgttt aatccgtgaa gcatcagcgc atccacgatg ccttcgcgca
    15481 tcgtcgcatt atcttcgatc acgagtatgc ggagagattt ggacggcgac atgaatcagt
    15541 tttgttcgtt gcggtacttg aggtaggtgt ctttattttt tggatccaaa gcgatcaaac
    15601 gatcatatat tttcttctca ccggagcgcg cgaaaatttt acaaaaatcc aaatagttca
    15661 gcgcgataaa tcgttccgaa tgatacgatg taatattttt agtgtaaatc cgctctaaga
    15721 tatccaacgc tttggttgca ttttgccgtg ctccggccgc atcctcattt tctagctgcg
    15781 atacggcttc atagaaatga tctttcataa tccggtattc ctggtaacgc agatcgagaa
    15841 aatcatcgga tttcaataaa cgtgcatccc aaccggaaac attttcaggg acaaatgctt
    15901 tagcctgcgt agccaaactc cgcgcttttt ggaaaaaagg cgtacctaat aatttgccaa
    15961 gcgaatccat ttcatcaccg agcatgatgt agatataaaa atctataata cttaaaaaag
    16021 gatcgatgac attctcatca tgataaaaaa cggcgccctt tcggatcgaa aaaatacatt
    16081 ctttatccag cgactgcatg acctttccat tggaaaaaaa catcgtggca tgcacacgtc
    16141 gtaattcatt tacttcagaa actttctcaa gaaaaatctg aatatccaca ggcatcggtg
    16201 tgttatcgcc tgttttggac caatcgtagt tattgacata cgaggtgatt ttctgttcca
    16261 ttccttcaaa taaatcgaga ttacgtccct gaagtttatc acggataatt ttgacattgg
    16321 ccgtcacttt ttgtgaaaaa gcagacgaac ctgatgccac aaccaacatg ctgaatagaa
    16381 cgaaccatcg tatcatgatc atattataat tgattaatat tttttacatc aatcatttcg
    16441 taggtgcctt ttcctatcca ggcaccgccg tcaaccgtaa tcacttctcc tgttatatat
    16501 cgggagaagt ctgaaagtaa aaacaaaagt gtatgggcca cttcttcggg ttgcccgaac
    16561 cgacccatcg gaatacgacc ggtcatacgt gacctctgtt ctggatcacc ccagaggttc
    16621 ttatcggtac cttccgtacg aattgctccg ggtgctatcg catttacacg aataccgtag
    16681 cgagcccact cgacagcgag cgtacgcgtc attgccagta cccctgcttt ggcgctggcc
    16741 gaatgaatga cgccgggctc cgatgcccaa gcatacgttg cgacaatatt ggttatcgta
    16801 ccgcccccgt tttgtatcat cgtttttcca acactgctcg aacaataaaa cgtaccattc
    16861 aaaacgatat tgatgacggc gttccaaccg ttgacgctca atttttcagc agcacacaaa
    16921 aaatttcctg cggcattatt gattaaatga tccacacggc caaattcttt aacggcttta
    16981 tcggtcatag cttgtacctg atccggtacg cgtacgtcgc atgggacggt taacacgcgt
    17041 gttcctgctc tggatatatc ctgcgccgcc gattccagca catccatccg tcgaccgctg
    17101 ataatgacat gcgctcctcg cgctgcacaa tgctcggcca tacttttgcc aagccctgta
    17161 ccgccacccg tgatcaaaac aactttgcca tccaaaaatt tttgttccag catagcgccc
    17221 cgataattat gatgccgacg cttctttgac gacatccgat tcaatgtaca aaatgcgtcc
    17281 gcaatattcg caattcgtca acttctcacg cttcttcaat tcgatgatga tctgcggtgg
    17341 tatctgctga aagcatcctc cgcacgacgc ttctttgact agcggcgcga cggcaatacc
    17401 gttttttgct ttgcgaatac gttcgtattt attgatcaga tcagggcgaa tttgcggttt
    17461 taagccttcg cgtttttgac gcaattcgtt ttcttcttta ctggtctctt cgatacgcgt
    17521 ttgcaattcg ccttcgcgtt cagaaaaatc tttctccagt cccgcaagac gttcttcttt
    17581 aggttttaca ccttcctgaa gttccataac ctgcgcatca atgtctatcg aacgcagctt
    17641 aatgcggttc aactcttgct cacaaaaatc aatctgagcc gatgtcgctt cgtattcttt
    17701 attggatgtg gccttgtata actgatcttg aaattttttg agtttctcct gattcatgga
    17761 ttgctcattg gtcagatctg atttttcttt ctcaaagttg gccacggatt gcttatccga
    17821 attgatttca tcgcggagtt cttccagttc cgctttgagt tgctccacga ttttaggtaa
    17881 atcgccgcgc agcatttcga tctcacctaa tttggtatca atgctttgaa gcgttaccag
    17941 atgggctatc atattttgca aagttctact cctttatcaa aaaaaaatgc actaattaag
    18001 attagtgcat acgctataaa aaagaaagac cggcgcaccc gttccgcatt gtgcggtcgg
    18061 tactttggaa gtattcgggt gcgctgatct cgtaaataca ttgcaggtta acgtcctgta
    18121 aatttaggag tacgtttttc gacgaaagcc atgagtcctt ctttggcatc accgcttttg
    18181 aaaagcgccg attgcaattc gcgctcaagg gagagtcctt gttcgagcgg aacttcaata
    18241 ccggtttgta ctgagcgttt gatatgaccg acggccatcg cggctttatt gggaatcgta
    18301 aactgtttgg cgtacgccat cacttgttcc ataaacgaag cgccgttgcc ttcatagata
    18361 tagttaacaa ggcccatatc gagcgcttct tcgtaagcaa atttacgtcc ggttgccatc
    18421 aattcgatag aaagcgaacg tccaacggca cgtgcaaaac gctgtgtgcc gccggtaccg
    18481 ggtaatacac ccagcgccac ttccggaaga ccgatcatac cggcatcttt acgcgcaatg
    18541 cgtatatctg cagccaatgc gatctctaaa ccgccgccaa cacagtgacc attcaatgcc
    18601 gcaatgacca acttcggcgt atgttcgaga cgcaataacg tttcattggc atgaagacag
    18661 aagtaatact tccatacagg atccgctttg ctaagcatgt caatattggc gccggcgcag
    18721 aaaaatttct cacccaaacc ggtaagcact atgacgttga cattttcgtc gaaacgcgct
    18781 ttgagaatgc actcatccaa ctgacgcatc atttcatgcg tataggtatt ggccggcgga
    18841 tccgtcagcg tcaatatggc gactctgtct tttacttcgt actgtactaa tactttctcg
    18901 gacatggtac tgctctccct gaaatggtta tttattttgt gaatgcgcta atgtcaaaaa
    18961 ttcggttctt gtgcgggcat cgtctttgaa tacgccaagc atacaactcg tcgtcgtaag
    19021 gctgtgttgc ttttccacgc cccgcatcat catacaaagg tgctgcgctt cgatgatcac
    19081 gccgacccct tcgggatgca aagcatcctg aatagcatta gcgatttgca tggtcaggcg
    19141 ttcctgcacc tggagacggc ggctgaatac gtcaacaact cgcggtaact tgctgagacc
    19201 gatgattttc cctttgggga tataagctat gtggcacttt ccaaaaaaag gcaacatgtg
    19261 gtgctcgcaa agactgtaca attcgatatc tcgaaccaat accatctcat taaaattttc
    19321 ttcaaaaatt gccgattgaa tgactgcttc agcgctttcc tgatagccgc ccataaggaa
    19381 ttcaaaactc ttcgccacac ggtccggcgt atcgcgtaaa ccttcccgat cgggattttc
    19441 accgtaatta cgaagcagtt gtgtgatcag ttcttgtgtt ttcatggtat caaataaaaa
    19501 accctgaatt cttaacaatt cagggtaaaa atttaagcaa accgttgagc ggtaactgtg
    19561 cgttcaaatg tcgtcttaaa attaaaaagt acgctcaaaa caatcatgat tttagtgaac
    19621 agttgttttt taaaaacagt tgaaaataga cgcctggcac ctataatgca agcaaaattt
    19681 atagatcatt ttttggccat aatgaccaaa caatcgccgc cttcttcatc catcgatttt
    19741 tgaaaaatat cgtacggata ggtaaaatct tttccgcggc gagtgccgac atcgttggta
    19801 atcacgcgat caggtgtata tccgatcacc gttagcatat gatacctcgg ccccggcggg
    19861 gtataaaaag gatttttaag cgtccgtccg aacgtcggcg ctatcacagg ataccccatc
    19921 gcgacataac gcttgatgtc ttcgattttt gctttacgta aaattttgac ctcttcgtct
    19981 ttgtagccaa aataacccat catcatcatt ttgacgctgt cggcatgaat gtctttgtgt
    20041 cctccaaaat gctgatcttg ccaacgcaac atatctttga tcaaccaatc cacagaatct
    20101 acagaaacat ctttgatgcc tttattatag aatacggctt gcaaaacggc cgcttcctca
    20161 cagctttctt tatgaatttt ccaactgtcc ggattcatat gcggcgcctg acaataaaac
    20221 ggtacttcaa gatacgcttt ttcaggaaac gaaaccggca tggtatccgc tacggcagcg
    20281 gcatggatga tatccgtttt agaaatttct ttaagcgcag cttcgttttc tttttccaat
    20341 tgtgtacgga ggcgatattc ggcaaaacgc ggatagagca tcacaccgaa aaccataaga
    20401 acaaccgtac ccaatccgat caaaattcgt ttattgatat tcatatttat tgccctgtct
    20461 tagtacgatc ttttggaaga atcacgataa tatcaccgcc gtgaaaatcc atcgcttttt
    20521 ggaatatatc atacgtatag gtataaccgt taccgcgcat ggttcctaca tcgttggtga
    20581 taattttttc agaatcaaaa ccttttaccg tcagcatatg ataacccgac atgcctcggg
    20641 cacgaagatt ttcgacatag tgcggattgt tgagcacgta accattggcc atcgcaatca
    20701 caggattacc tgcaagtatc tgatctttta tatcttgaat agttgctttt cggataatgc
    20761 gcacttcgtc atcggtataa ccgaagaatg acatgatcat ggttttagta ctgtcacaac
    20821 gaatatcgtg atggcctttg aattgcttca tctgccaatc aatcatatca cgtaaaattt
    20881 tatccgtagc gctcaggtca acttttttga caccgcgcaa ataatagacg acttgcaggg
    20941 ccgccgcctc ttcacagctt tcgcggtgaa tattccagct atccatattc ataaacggtg
    21001 cctggcaaaa aaacggaaca tccaacagag ctttttcagg cggtatttta ctaagcgaat
    21061 ctttcatagc ttgcacgaca gatgcgataa ccgatgaagt tttcggacca tcgttcttaa
    21121 tatcggagga cggcgactgc gccgaaacac cccatccggc aagaatgcca aacaggcaca
    21181 ctacaacgat cacaagtgtt ttcatacacc acatttcttg aatgatagac tgtaagatca
    21241 gggaaaatat aaaaaaacaa tcaggaattg caattggtaa ttcgttgcaa tgcttgcggt
    21301 atcgattcga tgatatcgcc cgccaaaagc ccgtattctg ttttgtctgt tacggcaata
    21361 tctccggcca agccgtgaat atacgctccg caaaagccgg ccgtttccga atctaatcct
    21421 tgcgccatca gccccgcgat aactcccgtc aatacatcac cgcttcctgc tttggccata
    21481 cccggattac cggtgtgtgt tacataaaca tgcgatttac cggcgatttg cgtcggaacc
    21541 cccttgagga gaatcgtgtg gtttaatttt ttttgccaat catggagttc atcattcatt
    21601 gcggtggcgc tgtttttttc aaaaccaaat aaacgacgga attcaccgct gtgcggagtg
    21661 atgatcatat ctgaatgtac atcgcgcaaa actttccatt gcccgtttaa aatattgaga
    21721 gcatccgcat ccaaaatcga aggaacactc cattgactca gcaatggaaa catccattca
    21781 gctatcgcgg gatttttaga tagacccggg cctatagccg cacaagtgca ccatccatgc
    21841 aaatcaacaa tcatagataa tgcggccggg cttattacgc catcctccgt ctccggtaag
    21901 tgtacggtca tggcttcagg caaaccttgt tcgatcacgt gcgctattga tttgggtatc
    21961 gcaacccgca caacaccggc acctgatcgc attgcggcgc gcgctgtcat caaagcagct
    22021 cccgacatgc cccgtgaacc ggcgataatg agtagtttgc cgaaatcgta cttatttgaa
    22081 ttatgtctgc gtataggtaa atggaacgcc acatccgatt gattaataaa ttgccataaa
    22141 ccgcccgaga gcaatacctc cgggaaacca atatctttta ttacaagatg cccggtgtat
    22201 ttttttccgt cattaaaaag caaccctcgc ttaggtaatc caatcgtcac agtgagatga
    22261 gcggtaaccg ccgtatctga aaacaatccg gtttcaccat caagtcccga aggtatatct
    22321 acggacacaa taaatgcgtt tttttgaaaa cgttgtttat tgatccattc gatccaagac
    22381 tttatgggtt cgctcaaatg cccgcggcca ccggtgccaa gtaatgcatc tacgatgacg
    22441 tcatattcct ccgttggtgc ttcaaacgca tcggaagcat cgactacgac gcctgttcgt
    22501 tccaattttt cgagatgcca cagtgcatca ccaccgatat gttttttctc cgcagcgatg
    22561 ataagttttg gcgtcatacc gccggaaatc atgcaccggg ctaacacata accgtctccg
    22621 ccattattgc cttttccgca aagaatgaga actcgtttcg atgctaaatc gggtatacgt
    22681 ttcgtcaatg tcaaaaaaat aacttcccct gcacgcgcca tcagttcgcg ccccgggata
    22741 ctatgacgtt cgatagtttc gcgatctatg gtctgcattt ctgcgggcgt gacgatacga
    22801 tgcatatatc agaaattacg ctggaattaa gtattaacgg aaaatacgat tcgctagaac
    22861 aaaatgccag attgatagac ttaaatgcgg gttttgtatt ttaaaatcat tccaaaacag
    22921 aatgtccctt aacgcgttac ttagtttcgt ataaataatt tgaatatgat caaataccgt
    22981 tctgatttta atttcttttt tttgtcaacc atgaaaagca cattagcaca aaatacactt
    23041 cgcctatttt attatctttt aaattaaaca gaatagtttc tcgatttact cctgacgtag
    23101 cgctgtcacg ggattatgat gtgcacctcg ccatgcttgc gacagtatcg tcaagccgac
    23161 taaaaatcct aataatatca cagccggtaa tataatacct atcccaaatt cagtacgata
    23221 cgcaaaagac tccagtatca tgttcgataa aaataaactc acaggcaatc cgatcagcat
    23281 ggctattccg atcagtttaa gatactgctt agataaaaga ttgattagct gcatagccga
    23341 agcgccgagt actttgcgaa taccgatttc acgggtgcgt gtctcgacac tgtaggaagc
    23401 cactccgagt aatcccagca tcgcgatagt caatatcagc aaagataata aaccgatgat
    23461 acgccccaaa tctgtaaaga tgctataaac gcttatcagt tcatccgaat aaacttgata
    23521 tggaaattcg atggacgggt taaatttttt ccatactgac ttaaaaaaag aaatcacggt
    23581 tttgtaattt tcgttcgtta ttttaaaatg aaccaatgac cattgcgcgg gttcatacgt
    23641 tagtatcacg ggctccattg cgtacgttac cggtttgaaa acaaaatctt tgatcacacc
    23701 tgcaatagtt acaggtcttg atttgtccag ataaataacc tttccgacgg catcgctatt
    23761 attagaaagc tgcagtgatt tgacaaattg ttcattgacg agaactttat cccctgtgct
    23821 ttcactccga tgcagattct cgcctgttaa taaagtaatt ttcatggttt caatgaaatg
    23881 ttcatcgacg ctataatctc gaacgacaga agcctctttg gttggatcag tctgaacgtc
    23941 taccgtgtga tcggcccatg tacccatcgc atgggacgca aaactataag aaactacgcc
    24001 tggaaaacgc gccatttcat cggcaatcac gcgtgcctgg gaacccgaaa gttgcacgtt
    24061 gatcgtattg cgccaatcga atccgtaata ctccattttt gaagcaaact ccgactgacg
    24121 atatactgta gtgaggacga taagaagaat aatggttacg acaaattctg aaataagaat
    24181 tgttttacga agcgtcaaac ctgaaaacat tcgaattttt gaaatatctt ttatgactac
    24241 cgccggattg aatgaggata aaaccatggc cggaataatt cctgccagta atccgatgca
    24301 caacgcaaat cccgcgaaca ataagtaggt tcctgccgag ggatcaaatc gaatatccag
    24361 ttccgaagta aaacttaaac tttggaaagc cggcacaaga aatacccgca gcagtgtttc
    24421 agctaccaaa aacgagacta atgtaatgat aaccgattcg cccatgaact ggaaaatcaa
    24481 ctgcgcacgc gttgcccctg aaactttgcg gataccgact tcccgcgcac gcgtcaatga
    24541 gcgcgccagt gtaagattgg catagttgaa taacgcggca aacattccta ttccggccaa
    24601 aaccatcaaa aaaataagaa ccgcttgcgg cattccgcct gaagtactat tggataacat
    24661 cggaccgggc gtgatcgctt gaagcgattg ggctttaaac gcataacccg catcccgggt
    24721 ttccaatgtt attccgcgat acactttttc gggaatccta tcaagtcccg cctgaagcat
    24781 tgccgcatcc gttcccggtg ttaccaccac gtatgtataa gtactgtagt aatttttcca
    24841 ttcttcgtgc gcaaggatac tgccgccgtt ccatgttttc agaaattcca aagaccccaa
    24901 tgcgtcgaac gtaagatggg atttattgtc catttttttt ataacacccc tgactacaaa
    24961 ctcaccatac gaccctatcg taacagttt
//
LOCUS       DAOQNQ010000017        24299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_700_length_24299_cov_6.302508, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000017 DAOQNQ010000000
VERSION     DAOQNQ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 24299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24299
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_700_length_24299_cov_6.302508"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..787
                     /locus_tag="PK754_02275"
     CDS             56..787
                     /locus_tag="PK754_02275"
                     /inference="COORDINATES: protein motif:HMM:NF022694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HNB08359.1"
                     /translation="MRVIQGVIFTLSALMAIVWMEPVSKAEAGGDSILIEDFTSSNTG
                     QFPKGWGWLDGTSVKKINEAKPGEVPYVVMEENGNKFLRADDKGQAITIVSDKKWNIK
                     KYQCIQWRWRVNQFPTGANEYAKGKTDNAASLYISYYVSFIGIPRSIKYIWSNTLPEC
                     ETFRKDGTGKATNVVVESGTSKTGQWITETINIYEQYKRVFGEYPPDEVAGIAIRTDA
                     DGTNSRAIADYDDIIAIPYCDGPCK"
     gene            844..1425
                     /locus_tag="PK754_02280"
     CDS             844..1425
                     /locus_tag="PK754_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNB08360.1"
                     /translation="MNFESIVESVSRSVQLNREEIEYFTSLLECKVVKKKNFLLMTGD
                     ICLYDYFVVSGCFKVCYSDDTGTEFIVKFALENWWVVDLESFINQTPSFFYIQAIEDS
                     KVLQLSRSNYDKLLRKIPAFHKFSNERWQNGFIALQHRIMHDQSMTAEERYRRFKEKY
                     PTLEQRIPQKLIAAYLGITSEFLSMIRKKILIS"
     gene            1511..1951
                     /locus_tag="PK754_02285"
     CDS             1511..1951
                     /locus_tag="PK754_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNB08361.1"
                     /translation="MKSFLFFADNNWLLLILRVTMGFVMLPHGAQKMFGWFGGYGFSV
                     TVNFFTETMRLPWLIAVLVILIEFFGAIGLILGFGTKVCAMSFIVVMLGAIVTTNYHH
                     GFFMNWFGNQQGEGFEYHLLVIGICAALLISGGGDYSLDRLLHG"
     gene            2444..3391
                     /locus_tag="PK754_02290"
     CDS             2444..3391
                     /locus_tag="PK754_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNB08362.1"
                     /translation="MIPFKNTHTMTVDEFAHVMENLRSYGAYVGFISGGETTIVDGLS
                     EMLLEAHKTFRFATTLVTGLYNKWENIEPAARVCMENGMHIQTSFDGLGEVGDHLRGC
                     KDFETVISENMRRISELRRDIQKKNKKKSVLYANTVMSDINLDQVPDIIAHVKSLGWK
                     STVGMYHTLTFSTRYNDDMVLRPGDRLNKTMTFLKQNPDVMNLDAWIDGVIPYVEDPN
                     LNWCPFVKAPYLATRTTIMENGDVHLCKGNPIGNVFRQSLEKIFESYEYKQRLDEYSR
                     CEGCWTTCYTQRYLSVRPKSISEAVSNIKKIKRNTTVEL"
     gene            3388..3582
                     /locus_tag="PK754_02295"
     CDS             3388..3582
                     /locus_tag="PK754_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08363.1"
                     /translation="MIRFTKRFYFGMPLSLAGILIAFNYISNYRTYAVVLFWTGIITG
                     IVGCYLMFKGIKSKKIKIEP"
     gene            complement(3589..3861)
                     /locus_tag="PK754_02300"
     CDS             complement(3589..3861)
                     /locus_tag="PK754_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HNB08364.1"
                     /translation="MARMVHCIKLNQELEGLDKAPIPGEIGKRVYEQVSKQAWKMFEE
                     HFKMVMNEYRLNLMDPRTDEIFKQQVQQFLFSGTAARPEGFVEPTS"
     gene            3951..5057
                     /locus_tag="PK754_02305"
     CDS             3951..5057
                     /locus_tag="PK754_02305"
                     /inference="COORDINATES: protein motif:HMM:NF022591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HNB08365.1"
                     /translation="MKSVSTYVKKTTRWYTRYQNVISPVSFLLGFVWDAFSSKRIDAM
                     DQNYLLMGYLFIAGILLLLLHAVKAEKIKTGFWVTYQKLLPLGFHFCLGNLYSNHVYF
                     YFQSAAGFKSLVFIAGLMVLLLANEFYKERIHQFYLQFVMFFLAAFSFFIFFVPLFTR
                     ELGYRSFLIGGCISLVFTMMLIFAVALIARARYRKLLIKSAGLMIVIYGVMNIFYFAR
                     WIPPVPLALRHVGIYHTMKKKDQRYELQYEKAEAWEFWRTSGDVYHWQPGDTVICFAA
                     IFAPTEIHTTIHYQWEQYLDDRNAWVLQDHQSHTLQGGRDAGYRGYSRKGVISPGAWR
                     VEITTEEGLLLGSQTFRVERMPMNYDRKLHTVVW"
     gene            5183..5497
                     /gene="rplU"
                     /locus_tag="PK754_02310"
     CDS             5183..5497
                     /gene="rplU"
                     /locus_tag="PK754_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNB08366.1"
                     /translation="MYAVVDIKGFQYKCEAGKSLVIPRVAEAEVGDKIVFENVLLISE
                     NGAAQVGQPTVKGAKVETTVIAHGKGDKVIHYRKYRRKGFQKKKGHRQPFTEVEVTAI
                     TK"
     gene            5508..5774
                     /gene="rpmA"
                     /locus_tag="PK754_02315"
     CDS             5508..5774
                     /gene="rpmA"
                     /locus_tag="PK754_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNB08367.1"
                     /translation="MAHKKGLGSTKNGRDSHGQRLGVKRYAGQTVLAGNILVRQRGTK
                     FHPGRNVGIGSDDTLFALIDGVVKFEHKDKTRLRVSVYPQEAKA"
     gene            5912..6835
                     /gene="mdh"
                     /locus_tag="PK754_02320"
     CDS             5912..6835
                     /gene="mdh"
                     /locus_tag="PK754_02320"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNB08368.1"
                     /translation="MKITVVGGGNVGATAAQRIAEKELANEVVLVDVVAGMPQGKSLD
                     MYESAPVERFDVRMVGTQGYDETAGSDIVLITAGLARKPGMSRDDLLMKNAEIIKGIT
                     EQIAKRSPNAIIIMVTNPLDVMTYVAWKVSGFPRERVIGMAGVLDSARFRSFIAMELN
                     VSVRDISAFVLGGHGDTMVPLPRYSTVAGIPLPDLMDKATIDRLVQRTRDGGAEIVKL
                     LGTGSAYYAPSSSAVEMIESIVKDQKRILPCAVWLQGEYGQKDVFCGVPIKIGSKGME
                     QIVQIKLTADEQAELNKSAQDVKDNIAKLTI"
     gene            6990..7574
                     /locus_tag="PK754_02325"
     CDS             6990..7574
                     /locus_tag="PK754_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNB08369.1"
                     /translation="MMTLSTPLNAQSWSTGFFSGVNFSDARHSYRGTTTQGYARPVVG
                     LFVERNFVSWIGYRGELLWIRKKTDARTDSSYARWSATVIEAPLLMVLQSSSDYWGVS
                     PIFYFGVKVGYAVKTHYIEINGNATDFEGQTDYANRWSAAGELGAAIDYAISPKLSVD
                     VAFRFSLGLTDMDPNEPRWSFHDRLFTLGVKFKR"
     gene            7643..9787
                     /locus_tag="PK754_02330"
     CDS             7643..9787
                     /locus_tag="PK754_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNB08370.1"
                     /translation="MPADTLNTTYRPRIGLVLSGGGARGMAHIGVLKILDELRIPVDV
                     IAGTSAGAIVGGMYASGMDVAEIEKFARDIDWKDMLNDKPSRDFRSFRKKTQENENLM
                     GFEVGFRSWNVVFPQGVIAGQKLSFTLKTITLPVSGIQDFDALPVPYRAVATDIATGE
                     RVVLRGGNLAEAMRASMSIPGVLAPVEINGRLLVDGMFVQNLPIDVAQAMGADVIIAV
                     DIGAPLMDRSHLNSVLGITAQVMNMVTTENVNQQAALLTNKDVLIRPDIGSVSVADYL
                     QADAIITDGEKAARQWIPMLRRFSVSDEIYNEFRKKIRTRRKTHDRIDFIRIGKNIGQ
                     DPRIYHRLKTQPGSSLNMKTLKADLTRIYALGDFEKVDFDIVTEDTSRGLLIDGRWKS
                     WGPNYLRFGLTMATDLEGHSSVNALADYTMTRRNQLGAEWKHLFRVGQNPTYFTEWYQ
                     PLDFKGNFFMAPYGRAQRFESGFYQQRVEIAQYNGYAAEAGHDFGIAFGNAGELRAGV
                     IWGHIDAHSTSNASRKSINAERSGIRIRTQIDMTDHPDFPKKGSIWQSAYFSSQKKWG
                     SDGAYQTLRGAGMVVHTWDRNSIMGAFEAGSDLGTSSPIYELSELGGFLRMSGYRPGQ
                     IKGRHFGMTRCMYYRRLSAPNASYTEGLYAGFSVETGNAWDNHSQITSRDLKFSGAVV
                     VGIDTVVGPLYLAYARAPHQEGIVYIMIGRPF"
     gene            9872..11242
                     /locus_tag="PK754_02335"
     CDS             9872..11242
                     /locus_tag="PK754_02335"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HNB08371.1"
                     /translation="MSWFLRLSAMLWMVGVVNGVAGGEDLLQAGPMLGYAEKREVMIW
                     VQTKKSAEVRIRYWSLEAVKTQDQKKISKISEPIRTAKESDFTAHIVLEALEPGTRYG
                     YEIVLDGKTPTLPYANSFQTQALFEWRTDAPDFTLAFGSCLYVNDPPYDRPGRAYGNT
                     SEIMRVIGNVKPDLMLWIGDNVYLREVDWNSRSGILYRNRHTRAIKELQPLLGNTHNY
                     AIWDDHDFGPNNSDRSYAHKDHTLDAFKLYWANPNYGLSESPGVFFQFEWQDIDFWML
                     DDRYHRAPDDMMDGPDKTMLGKTQLQWLKDGLLFSNAPFKVIVCGNQVLNANTTYESY
                     NFYKNERADLLKFIKDNGITGVVFLSGDIHHSELLKWEDPDFYTLYDFTSSPLTSGIS
                     SGDTVGTNPQLVDGTKITDAHTFGVIRFSGKRRDRVMTMETWDEHNKKRWSFSIHEND
                     LHPAKK"
     gene            11290..12570
                     /locus_tag="PK754_02340"
     CDS             11290..12570
                     /locus_tag="PK754_02340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB08372.1"
                     /translation="MKKYILGSIVLCLCVGALYLWKGNGSDQTKRNASGSALVIRPAA
                     LERGRLNVDVSATGVINPINVVEVKSKASGLVEKIPIEASDVVHSGQLIARLDQTDTR
                     NALEQAIADSQVASAVLIQQENNWRRAKDLFEKNLLAQSEYDQINVEYVRAKATLVKS
                     QSNLVLARQKMAETTVRSPIDGIVLSRTVSEGQIVASAVSNAGGGTTIARLAAMDRVY
                     LTAAVDEVDIGKIRVGQRGRIVADAYPGDVFFGEVVRVAAQSTVIQNVTTFDVILLVP
                     NRGNKLKAGMNATVTVDIAGKDHALLIGNDLLRTYAEVQADKKILEEAGVVLPEKRGD
                     RTQDSSGRGMGRKRMREQNSGSDSTRLRFVVLSEAGTLRAHPVRIGLSNADVSEVLSG
                     LDDSSRVMLVVYSQALKESERFKDRMKERTSFSR"
     gene            12610..13827
                     /locus_tag="PK754_02345"
     CDS             12610..13827
                     /locus_tag="PK754_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB08373.1"
                     /translation="MNILESLRSALWALTSNRLRTFLTMLGIIIGVGAVITMVAIGEG
                     SRRRIQERLQGLGTNQLLVRPGSQNVGRTVLAAGSSQTLTEEDAETILKTSEHVSAVS
                     PELSRNAQVKYESKNTNTTITGTRIEFLKVRNFVLAEGRYFSAEEIKGNVKVAVLGQT
                     VEETLFGSAQSVGSIIKINGQNFTVIGVLAGKGQTGMGQNQDDQIMVPLTTAQTRLFG
                     LDFVTTIAVQVYDENMLDETTFDIERALRKTHKLRGDAENDFSIRNQADILATAQETS
                     DTMAILLASIAAISLIVGGIGIMNIMVVSVTERTKEIGIRKAIGAKRKDILLQFLIEA
                     MVICLFGGLIGVGIGIGSAYVLQWNAGWRTVVTPDSVLLSFGLSVLVGIFFGFYPALK
                     ASQANVIDALRYE"
     gene            13908..15176
                     /locus_tag="PK754_02350"
     CDS             13908..15176
                     /locus_tag="PK754_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNB08374.1"
                     /translation="MKKLVMLLMLGVGFNVYAGDKIPGFVYGDVKSVLNAAREKKQVV
                     MIDFYTAWCGPCKLLEKNVYRSAAFLPVTEKMVCYKIDAEKGEGIELAKKYNITGYPS
                     VVFMYADGKEIERKVGYNEDIEGYIADLMRIAEAKDVLPVWMAEYNANKNLTLGEKIV
                     RYYLSVDPRSAEPFYTELMAMPGMSDEQSAALKQQHAYRGLDYGLESAAKEADEIIRT
                     SKVEDDYDLISLAFNYAFYLTRRNQKEKAWEIASALYAKLDAEKRQSYAENFMEIKYR
                     SGQTDKSEDETRLRGIDLSDANGVSEAVKIHIKWKEKERAENVLKTWLSSYGTNASLD
                     DMNNVGWTAFEVKIMMREFAAAMIKTWDETVLEYQDAYKADTIANLCEAVGDKANAVR
                     FGQLAVNMIPERSRMRKEFEDNLKRYQAMP"
     gene            15479..16954
                     /locus_tag="PK754_02355"
     CDS             15479..16954
                     /locus_tag="PK754_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017325113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNB08375.1"
                     /translation="MPAPADVKIFDVKNTKLFINNTFVDAASNRTFETINPATGKAIC
                     AISEGDKNDIDTAVAAARKAFSDGSKWSRMSGSRRGKLLWKISELITKNADELAYLET
                     VDCGKPISETKNIEIPVTADIFAYYAGAATKIEGETIPVNGNYFNYTLREPLGVLGLI
                     IPWNFPLITAARKIAAALAAGNTVVVKPSEFTPLTTLKLAELFAEAGFPEGVFNVVTG
                     FGKTAGAALVAHADVDGIAFTGSTTTGQEIMKTASLTMKRLSLELGGKSPNIVFADTD
                     LDTTIKMAAAAIFYNKGEVCTAGSRLLIEESIHDEFIEKLAARAAKMMPGDPLNPETK
                     LGPLTSELQMNKVMSYVQAGKSEGAALLAGGNRIGNDGYFFQPTVFGNVDTSMKIATE
                     EIFGPVLATMKFKDFDDVVEKANRTYYGLAAGIWTRDIKKAHTLAKKIKAGTVWINTY
                     NMYDAAMPYGGYKMSGFTRECGMEAIYEFYTQVKSVWVDLN"
     gene            16951..17328
                     /locus_tag="PK754_02360"
     CDS             16951..17328
                     /locus_tag="PK754_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08376.1"
                     /translation="MKKEFLYISVLMWSFVVSGWAQKETPKAAPKSYVAAVFTIGKNW
                     DTTKTAYEQPHFKEHGANLKRLHAEGKIALGARYGEFGLVVFKTENMEEVRACFDQDS
                     IVIKDILRMDIQPFRPFYKGCID"
     gene            17371..18681
                     /locus_tag="PK754_02365"
     CDS             17371..18681
                     /locus_tag="PK754_02365"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB08377.1"
                     /translation="MIISKFFFRRFFWGAVAVSVILTGSLSAQTGRQLIDKIAAVVDD
                     EAIMMSEVQQYAYFEAMNLHINPSNTEEFDKIMKRVLETMIQQKIMLAQARFDSITID
                     EAQVEQSLEQQIRERVQQAGGEDELERAMGKSVKELKRLYRPNIRKQMLAQRIQSTKF
                     NDVKVSRSEIEKFFEEFQDSLPQIGASVKFSQILLEVKAGDEAFAEARRMAQMYLDSI
                     KKGADFATIAKAVSTDPGSGKKGGDLGWFNRNDFVKEFADAAVRLEPGEISDVVKSEF
                     GYHIIQMIEKSGERIHVRHILLGVANGPEDKTRTREKLGRIRQDILEGKISFEKAALQ
                     YSDDPAKKGNLGNMGWIEIATLQDKSFVKALDPLKNEEISEPFETPFGFFIVQLHDRR
                     DTRKLNLNEDWQTIENFALRQKQAKEMDKWLEKIKSKFFIDVRL"
     gene            18704..19243
                     /locus_tag="PK754_02370"
     CDS             18704..19243
                     /locus_tag="PK754_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08378.1"
                     /translation="MEFISAQWFLWRSRFLKPTEVMDFPQRLSEIKRVLVYFPMAIDK
                     SRAETIFGQIQKTLSGCTLDFLLHPETPQEVSAFLTERQATVTILAKTDFGLLRTLNG
                     EAVDRIRAKEYDMILDFTFTADWAMAWALRACGVSVLAGAFTEPRSERFHNLLIKAHD
                     APTFDATLFGCLAQMKTVI"
     gene            19275..20627
                     /locus_tag="PK754_02375"
     CDS             19275..20627
                     /locus_tag="PK754_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08379.1"
                     /translation="MSQRAKQWTYHLSVAVLAIGMVVVFNFLVCHPVDFVRTNTKPAE
                     WKTYANTKFGFSVEIPSTWSIREDAQGVELYDAETRRYISVKALAEESSLLNIPTDEY
                     TIERSVLRHTYVCDTIREPHRVVTKSGLTGYMIAPPLRNDVDRSQINAPWADRCDAST
                     LSLTYFPVQFAGDIYASFVEFLSTGPSDAVYEHMTQSYRHLFKTLRQGELMRMHLIDT
                     SETPSPAVAYVGETRGFYVDFDADGTDELLIAGMPRNMEDTKERCFFRVLKKQGDQWV
                     WAFERKYSENSFHSSDIKIVNLDNRFGPDVFLRFVEHGNPWGKNSTVIIFWDGDKFRG
                     ADFGAFGDARDLNGDGTDEVIHTTNSYFSQGAIASWYDVYTYDKGEFIEVNSRFPEFY
                     KNTVLPKYRDQYETVQGELALTRVEQFKGPARQLMFRLSRYIDMSTDISEGRPARERW
                     "
     gene            20676..21446
                     /locus_tag="PK754_02380"
     CDS             20676..21446
                     /locus_tag="PK754_02380"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNB08380.1"
                     /translation="MSMSFRDIVVLFEDEHFIAVHKPSGLLTIADRFDTKAPNLADLL
                     RDERGFVFKVHRLDKPTSGVVLFAKHKEAHQSVSLQFEKHTIQKNYLALVSGRVQEKQ
                     YNVDVPIGEAERGLMKIDRTHGKPSETEFSVIESFTRWTWLEARPKTGRTHQIRIHCL
                     AKGTPIVGDMHYGSETGIFLSQLKKNYKRTGDSDEKALPGRLALHAAALTFLHPWENR
                     PITVSASLPKDLQSTLNQLRKYAAVAASSHHESGALMM"
     gene            complement(21414..22196)
                     /locus_tag="PK754_02385"
     CDS             complement(21414..22196)
                     /locus_tag="PK754_02385"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HNB08381.1"
                     /translation="MRKTWQWIDTGKQPGSFNMAFDEYLAAEVGSGLRPTTLRFFQWQ
                     PYCISLGKHQNISEINSEKAHHDGLEIVFRPTGGRAILHAQELTYSIVYHDDIRGSVE
                     ESHAHISTAIAIALRRLGYEAYCTTEQTDFKQHYRNPSSALCFSSSAKSEIQIEGKKV
                     VGSAQRRYAQATLQHGSILIGDFHRKLADYIHADAATKNILQRVMNEKTTELNQYIAV
                     DIAALKESLREAFSEFFSIDWTDGAWQADAIASHHQRSAFMV"
     gene            22284..22895
                     /gene="pgsA"
                     /locus_tag="PK754_02390"
     CDS             22284..22895
                     /gene="pgsA"
                     /locus_tag="PK754_02390"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNB08382.1"
                     /translation="MRFIPNILTILRILLTPVFVFCLLSDEYQVYSVALFVFASVTDW
                     VDGYLARKYSYHSGLGKFLDPLADKILVLSAFFSFVYLGFVKLWMVMVIVIRDFMITS
                     LRSYAIRRGKPMMTNFFAKLKTAFQMLMIGLILIFIAMQSLIHMTISEQLNYFIYIVT
                     HYEVIYVGMLVVTAVTVISGLLYLYENRESLRSLHSPSQVKNT"
     gene            22901..23359
                     /locus_tag="PK754_02395"
     CDS             22901..23359
                     /locus_tag="PK754_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNB08383.1"
                     /translation="MLNAHKLLGTALGVGYLPKAPGTWGSLLALAIYFIFFPSSSSLL
                     IHAVFFIIIAAVFGLGVVSATRLEKVYGHDPSCVVIDEVVGLWIALWFLPKTWIVMIG
                     AFVLFRFFDITKWFGINRLQDLPKGWGVMMDDVLAGIWANVILQIVVRIY"
     gene            23385..>24299
                     /gene="sucD"
                     /locus_tag="PK754_02400"
     CDS             23385..>24299
                     /gene="sucD"
                     /locus_tag="PK754_02400"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNB08384.1"
                     /translation="MSVLVDKKTRLVVQGITGKEGTFHTSQMTEYGPYVVAGVTPGKG
                     GLVYKGNEKDKLKYGVPVFNSVKDAVEKAKANTSVIFVPPAFAADAIIEAAEAGIKVI
                     ICITEGIPAADMVRAYNFIKDKGVHLVGPNCPGVISPGKCKVGIMPAFIHKPGRVGVI
                     SRSGTLTYEAVGQLTALKIGQSTCIGIGGDPVIGTRFVDAFKLFAEDKDTDAIIMIGE
                     IGGTAEEEAAAWVKKNFKKPVVAFIAGQTAPPGRRMGHAGAIISGGKGTAEEKMKALK
                     SAGIHVVKSPADLGVTVAQVLGIKVKSTT"
BASE COUNT         6602 a         5010 c         6069 g         6618 t
ORIGIN      
        1 acttttaaaa tgacatttaa tacataaatg atatgaaaac aaagcggggg aatatatgcg
       61 cgtcatacaa ggtgtaatat tcacattgag tgcgttgatg gctatcgtat ggatggaacc
      121 ggtttcaaag gccgaagcgg gtggcgattc tattttgatc gaagatttta cttcgtccaa
      181 taccggtcaa ttcccgaaag gttggggatg gctagacggg acgtcggtta aaaagataaa
      241 tgaagctaaa cccggtgaag tgccgtacgt ggtgatggaa gaaaacggta ataaattttt
      301 gcgtgccgat gataaaggac aagccatcac gatcgtgtcc gataaaaaat ggaatatcaa
      361 aaaatatcaa tgcatccaat ggcgctggcg cgtcaatcaa tttcctaccg gcgccaatga
      421 atatgccaaa ggtaaaaccg ataacgcggc cagtctgtat atcagttatt atgtgagttt
      481 tatcggcata ccgcgcagta tcaagtacat ttggagtaat acacttccgg aatgcgaaac
      541 tttccgaaaa gatggtacag gaaaggcgac aaatgtagtc gtggaaagcg gcacatccaa
      601 aaccggtcaa tggattacag aaacaatcaa tatctacgaa caatataaac gcgtattcgg
      661 tgaatatccg ccggatgaag tcgccggtat agcgatacgt acggatgccg atggcaccaa
      721 ttcgcgtgcg atcgccgact acgatgatat tattgccatt ccgtattgcg acggaccttg
      781 taaataaatt tataacatga tttattactc gcatcagttt ttataaaaaa acagcatttt
      841 ctcatgaact ttgagtctat agtcgaaagt gtatcgcgat ccgtacagtt aaatcgggaa
      901 gaaattgagt attttacttc gttgcttgaa tgcaaagttg taaaaaaaaa gaattttctg
      961 ctcatgaccg gtgatatttg tctgtatgat tattttgttg tcagtggttg ttttaaggta
     1021 tgttatagtg acgatacagg aactgaattt atcgtaaagt ttgctttaga aaactggtgg
     1081 gtcgttgatc tggaaagttt tatcaatcaa acgccttctt ttttttatat tcaagctatt
     1141 gaagactcca aagtacttca actcagtaga agtaattacg acaagcttct gagaaaaatc
     1201 cctgcatttc ataaattttc aaacgaacga tggcaaaatg gctttattgc acttcagcac
     1261 cgaattatgc acgatcaatc aatgacggct gaagaacgat accgacgttt taaagaaaag
     1321 tatcccacac ttgaacagcg tattcctcaa aaactcatcg cggcttattt aggtattaca
     1381 tctgaatttc tgagtatgat tcgcaaaaaa atactgattt cttaaactag ttcaatgatt
     1441 ttgtgtaaca gcattgaata ctataggtgt gaaaataaat cttattttat cacatcaaaa
     1501 aggtgctgtc atgaaatctt ttcttttttt tgcggacaat aattggttgc ttcttatttt
     1561 gagagtcaca atgggcttcg taatgctacc tcacggggct caaaaaatgt ttggttggtt
     1621 tggtgggtat ggtttttcag tcacagttaa tttttttact gaaaccatga gattgccttg
     1681 gctgatcgca gtactagtta ttctaattga attttttggt gctatagggc ttattctcgg
     1741 ttttggcacc aaagtatgtg cgatgagttt tattgttgta atgttaggcg ctatcgtaac
     1801 aactaactat catcacggtt tctttatgaa ttggtttggt aatcagcagg gggaggggtt
     1861 tgaataccat ttactggtga tcggaatttg tgcggcattg ctgatcagcg gcggaggtga
     1921 ttactcgtta gacagattgc ttcacggtta aattgcattt tttttataca acgatgttct
     1981 ttgcaacgga atcttggttt tataaaaaaa atgctgaata aatactggtt attcatatct
     2041 taaatattct taagttcgat accctaacta ccaaaacggt attatttaat actgttttgg
     2101 tagttttgac ttcataagcg agaatattta gcattggttg atattggaaa tcgaagtatt
     2161 tatacaatat cagctttttt aataaagaat ggatcgatgg tgaaaaaatg aatttgtttt
     2221 ttattaaaaa taattataag aattacaatt gatccgacta acttgcagta tggaacgcat
     2281 gtcgtctata acgatgcagc cataaaataa agcgagtgca tccattttga tgccaaaata
     2341 aagaatgttt aaaatactta ataatatcac ccaccggcgc cctgttaact ccagcaccga
     2401 agttcggata acctacaagt gtacgcagcg ttgtcggcag tgtgtgattc ctttcaaaaa
     2461 tactcatacg atgacggtgg acgaatttgc gcacgtcatg gaaaatctgc gcagttacgg
     2521 agcgtatgtc ggcttcatat cgggcgggga gacgacgatc gtggatggtt tgtcggagat
     2581 gttgcttgaa gcgcataaaa cttttcgttt tgcgaccaca cttgtgacgg ggctttacaa
     2641 taaatgggaa aatatagagc cggccgcacg ggtttgtatg gaaaacggaa tgcatattca
     2701 gacatcgttt gacggtttgg gggaggttgg ggatcatttg cgcgggtgca aagatttcga
     2761 aacagtgatt tccgaaaaca tgcgcagaat aagtgagctg agacgagata tccaaaagaa
     2821 aaataaaaag aaatctgtac tttatgccaa tacggtgatg agtgatatca atttagatca
     2881 ggtccccgat atcatcgcgc acgtcaaatc tttaggatgg aaatcaacag tcggaatgta
     2941 ccatacgttg actttttcga cacgttacaa tgatgatatg gttttgcgtc ccggcgaccg
     3001 attaaataaa accatgacgt ttttaaagca aaatcccgac gtgatgaatt tggatgcttg
     3061 gattgacggc gtcattcctt atgtggaaga tccgaattta aattggtgcc cgtttgtaaa
     3121 agcaccgtac ttagcgacac gcacgacgat catggaaaac ggcgatgtgc atctctgcaa
     3181 gggtaatccg ataggaaatg tttttcggca atcgttagag aaaattttcg aaagttatga
     3241 atacaaacaa agattagatg aatacagccg gtgcgaaggt tgttggacta cctgctatac
     3301 gcagcggtat ctgtcagtgc gacccaagtc catcagtgag gctgtttcaa atattaaaaa
     3361 aatcaaacgt aatacgacgg tggaattatg atacgtttta cgaagcgttt ttatttcggt
     3421 atgcctttgt cgctcgcggg catactgatc gcattcaatt atatcagtaa ctaccgtacc
     3481 tatgccgttg ttcttttctg gaccggtatt ataaccggta ttgtcggatg ttacctcatg
     3541 tttaagggca tcaaatcgaa aaaaataaag atcgaaccct gatacgattt acgatgtagg
     3601 ttcgacaaat ccttccgggc gggctgctgt accgctaaaa agaaactgct gaacttgttg
     3661 tttgaatatt tcatccgtac gcggatccat cagattgagg cggtattcgt tcattaccat
     3721 tttgaaatgt tcttcaaaca ttttccatgc ttgtttagac acctgctcat atacccgttt
     3781 gccgatttca cccgggatcg gcgctttatc gagaccttcg agttcctgat tgagttttat
     3841 acagtgcacc atgcgtgcca tagatcatac tccgaattgt tgttgatata taaaccaaac
     3901 gcgctgaaga tagcggattt gataaaaaaa acaaatacta tcgcacgctt atgaaatcag
     3961 tttcaaccta tgtaaaaaaa acaacccggt ggtatacgcg ataccaaaac gtgatttctc
     4021 cggtttcgtt tttactcgga ttcgtatggg atgctttttc ttccaaacgc attgatgcaa
     4081 tggatcaaaa ttacctgcta atgggttatt tgttcattgc gggtattcta ttgctgttat
     4141 tgcacgccgt aaaagctgaa aaaatcaaaa cgggtttttg ggtgacgtat caaaagcttc
     4201 ttcccttggg ttttcatttt tgtttgggca atctctacag caaccatgtt tatttttatt
     4261 ttcaaagtgc cgccggattc aaatcattgg tctttatagc cggacttatg gttctattgt
     4321 tagccaatga gttttataaa gagcgcatcc atcagtttta tcttcagttt gtgatgtttt
     4381 ttttggcggc attctctttt tttatttttt ttgttccgct gtttactcgt gaattaggtt
     4441 atcggtcctt tttgatcgga ggatgcatca gtttagtgtt taccatgatg ctaatttttg
     4501 ccgttgcgct tatagcgagg gcgcgatatc gcaaactctt gatcaaaagc gccggactga
     4561 tgatcgtgat ctatggggtt atgaatatat tctactttgc ccggtggatt ccgccggttc
     4621 cgcttgcact gcgtcatgtg ggtatttatc acacgatgaa aaaaaaggac caacggtatg
     4681 aattgcaata tgaaaaagcg gaggcatggg agttttggcg cacatcgggg gatgtgtatc
     4741 attggcaacc cggtgacacg gtaatttgtt ttgccgcgat ttttgcccca accgaaattc
     4801 acaccacgat acattaccag tgggagcaat atttagacga tcgtaacgcg tgggtattgc
     4861 aggatcatca gagtcatact ttgcaaggcg ggcgcgacgc cggatatcgt ggatactctc
     4921 gaaagggtgt gatttccccc ggggcatggc gcgtcgaaat aactacggag gagggtctgt
     4981 tgctcggttc gcagacgttc cgcgtcgaac gaatgccgat gaactatgac agaaaactgc
     5041 atacggttgt gtggtaactc ctcttagcga ggagtaaaag cgctcaaaaa agcctatcta
     5101 tttttgataa aaaaatcctt gcgttcctat agttcgtcgc ctatattggc gtccctaatt
     5161 aactaacgtc caggaggaca cagtgtacgc agtcgttgat atcaaaggtt ttcagtataa
     5221 gtgcgaagcc ggcaaatcgc tggtcattcc gcgcgtagct gaagcggagg ttggcgacaa
     5281 gatcgttttt gaaaacgttc ttttaatctc cgaaaatggc gcggcccaag tcggtcagcc
     5341 taccgtaaaa ggtgcaaaag tcgaaacgac ggtgattgcg cacggcaaag gtgacaaagt
     5401 catccattac cgcaagtatc gccgcaaagg cttccagaaa aagaaaggcc atcgccagcc
     5461 cttcacggaa gtcgaagtaa ccgctattac gaagtaagga gagttccatg gcacacaaaa
     5521 aaggtcttgg aagtacgaaa aacggccgcg acagccatgg ccaacgcctc ggtgtgaagc
     5581 gctacgccgg acaaacggtt ctcgccggta acattctggt tcgccagcgt ggtaccaaat
     5641 tccatcccgg ccgcaatgtc ggtattggaa gcgatgatac gctgtttgcg ctcattgacg
     5701 gcgtcgtgaa gtttgaacac aaagacaaaa cgcgtttacg cgtgagcgtc tatccgcagg
     5761 aagccaaagc ttaagccgcg gtacctgtgc agagctgtat aacggacaac gtcgtcttcg
     5821 gacacgctgt ccgttttttt ttgttttctt ggatgaattg tgtatcatac gtgcgttcat
     5881 cttttaacat ttatatttca ggagattgta tatgaaaatt accgtcgtcg gcggaggcaa
     5941 tgtcggtgcg acagctgccc aacggatcgc cgaaaaagaa ttggccaatg aagttgtact
     6001 cgtggacgtc gtagcgggta tgccccaagg taaatcgttg gatatgtacg aatcggcccc
     6061 ggtcgagcgt tttgatgtgc gtatggtggg tacgcaaggt tatgatgaaa cggccggttc
     6121 cgacatcgta ttgatcaccg ccggtttggc ccgtaagccc ggcatgagtc gcgacgatct
     6181 gctgatgaaa aatgccgaaa tcatcaaagg cattacagaa caaattgcca aacgttcccc
     6241 gaatgcgatc atcatcatgg taaccaatcc tttggatgta atgacgtatg tagcctggaa
     6301 agttagcggt tttccgcgtg agcgtgtcat cggtatggcc ggtgtgctgg acagcgcacg
     6361 tttccgttca tttatcgcga tggaactcaa tgtgtccgtc cgcgatatca gcgcatttgt
     6421 acttggcggc cacggggata cgatggttcc gttacctcgt tattcgacgg ttgccggtat
     6481 tcctctgccg gatttgatgg ataaagctac cattgatcgc cttgtccaac gcacgcgcga
     6541 tggcggcgcc gaaatcgtca agctgctcgg aaccggttcg gcttattatg ccccttcatc
     6601 ttcggctgta gaaatgatcg aatccatcgt caaagatcaa aaacgcattc ttccgtgtgc
     6661 cgtatggctg caaggcgaat atggccagaa agatgtattc tgcggcgtgc ccatcaagat
     6721 cggaagcaaa ggtatggagc agatcgtaca gatcaaactg acagcggacg aacaagctga
     6781 actgaataaa tctgcacaag atgtcaagga taacattgcc aagctgacaa tataagttga
     6841 tagtaaatta attttttcaa aactccctgc ctttagaaag gcagggagtt tttgtttgta
     6901 gatatgtata ggcatgatta cattcgtgta gcataattat ttctcccact ccgatgtaca
     6961 aaatcaatac catatttcct ctcgtatgta tgatgacgct gagtactcct ttgaacgcac
     7021 agtcatggtc gaccggtttt tttagcggcg ttaatttttc cgatgcgcgt cattcgtatc
     7081 gcggtactac gactcagggt tacgcgaggc cggtcgtcgg gttattcgtg gaacgtaatt
     7141 ttgtttcatg gatcggatat cggggtgaat tgctttggat tcgaaaaaaa acggatgcgc
     7201 gaacggatag cagctacgcg cgatggtcag cgacggtcat cgaagcgccc ctgctgatgg
     7261 tgttgcagtc tagttcggac tattggggcg tatcgccgat attttatttt ggcgtaaaag
     7321 tcggttatgc ggttaaaaca cactatatcg aaataaatgg gaatgctacg gatttcgaag
     7381 gtcaaaccga ttatgccaat cgttggagtg cggccggaga gttaggcgcg gctatagatt
     7441 acgccatttc gcctaagtta agtgtagatg tggctttccg tttttctctg ggtttgacag
     7501 atatggaccc gaatgaaccg cgttggtcat ttcacgatcg cctgtttaca ttgggagtaa
     7561 aatttaagcg atgattttcc tattgaaaaa aactttatgc attgttatcg cgggtgtttt
     7621 ggccgctcct ttcttgatgg gtatgcctgc tgatacccta aatactacgt accgtcctcg
     7681 tatcggcttg gtcctcagcg gcggcggggc gcgcggtatg gcgcatatcg gtgttttaaa
     7741 aattttggat gaattgcgta tacccgtgga tgtgattgca ggaaccagtg ccggcgctat
     7801 cgttggcggg atgtatgcgt cgggtatgga tgtcgcagag atcgaaaaat ttgcgcgcga
     7861 catagattgg aaagatatgt taaatgacaa accgtcccgt gacttccgtt catttagaaa
     7921 aaaaacacag gagaatgaaa acctcatggg cttcgaagtg gggtttcgtt catggaacgt
     7981 cgtatttccg caaggtgtga ttgccggtca aaaattaagt tttacgctca aaacaatcac
     8041 tctaccggtc agcggtattc aggatttcga tgccttgcct gtgccgtatc gggcggttgc
     8101 tacggacatt gccaccggtg aacgtgtagt ccttcgaggc ggtaatttag ccgaagccat
     8161 gcgcgccagc atgtctatcc ccggtgtttt agcgccggtg gagatcaatg gaagattatt
     8221 agtagacggt atgtttgttc agaatttgcc tattgatgtg gcacaggcga tgggcgcgga
     8281 tgtaattata gcagtagata tcggagcgcc actgatggat cgctcgcatt tgaattcggt
     8341 actggggatc acggcgcagg tgatgaatat ggtgactacg gaaaatgtga accagcaagc
     8401 cgcattactt acgaataaag acgtattaat acgaccggat atcggttcag tttccgtcgc
     8461 cgattatttg caggcggatg ccattattac cgacggcgaa aaagccgcgc ggcagtggat
     8521 tccgatgttg cgtcgttttt cggtgagcga tgaaatctac aacgagttta ggaaaaaaat
     8581 tcgcacgcgc cgcaaaacac acgatcgtat tgattttatc cgcatcggta aaaatatcgg
     8641 acaagacccc cggatttatc atcgtcttaa aacgcagccc ggttcttcgc tgaacatgaa
     8701 aacgctcaaa gccgatctga cgcgtatcta tgcgctcggt gattttgaaa aagtggattt
     8761 tgatatagtg accgaagata cgagtcgcgg acttttgatt gatgggcgat ggaagtcttg
     8821 gggccccaac tatctgcgat ttggcctcac gatggctacg gatttggagg gtcattcgtc
     8881 tgttaatgcg ttggcggact atacgatgac gcgacgcaat caactcggcg ccgaatggaa
     8941 acatcttttt cgtgtcgggc aaaaccctac ctactttacg gaatggtatc aaccgttaga
     9001 tttcaaagga aattttttta tggcgccgta tgggcgtgct caacggtttg aatccggatt
     9061 ttatcagcag cgcgtcgaga tagcgcagta caacggatat gcggccgaag ccggtcatga
     9121 ctttggaata gcatttggga acgccggcga actgcgtgcc ggggtaattt ggggtcacat
     9181 tgatgcccac agcacgtcaa acgcatcacg aaaatccata aatgccgaac gctcaggaat
     9241 tcgtatccgc acacaaattg atatgaccga tcatccggat tttcccaaaa aagggagcat
     9301 ctggcagagc gcgtattttt catcacaaaa aaaatgggga tcggatggcg cttatcagac
     9361 tttgcgcggt gccgggatgg tcgtgcatac atgggatcgt aattcgataa tgggagcgtt
     9421 tgaagccgga tccgatctcg ggacatccag tcccatatat gaattgtccg aactcggtgg
     9481 ttttttgagg atgagcgggt accgaccggg acagatcaaa ggacgtcatt ttggaatgac
     9541 gcgttgtatg tattaccgcc gactatcagc gccgaatgca tcttacaccg aaggtctgta
     9601 tgccggattt tctgttgaaa ccggaaacgc gtgggataat cattcgcaaa tcacatcgcg
     9661 tgatcttaaa ttttcgggag ccgtagtggt tggcatagat acggtcgtag gtccgcttta
     9721 tcttgcgtat gcgcgtgcgc cgcatcagga gggcatcgta tatattatga tcggtcggcc
     9781 attttgaaat ttttaaaaaa cactatgctt cattaggtca ggcgtttata ttagagccga
     9841 atcaaataat cttctgaaag gtttcttttt tatgtcttgg tttttacggt taagcgcgat
     9901 gctctggatg gtcggtgtgg ttaacggggt tgccggaggt gaagatttat tgcaagccgg
     9961 tcctatgctc ggttatgcgg aaaaacggga agtgatgatt tgggtacaga ccaaaaaatc
    10021 tgcggaagtg cgcattcgat attggagttt agaagcagta aaaacgcagg accaaaagaa
    10081 aatttcaaag atttcggaac caattcgtac tgcgaaagaa tccgatttta cggcccatat
    10141 cgtgctcgaa gccttagagc cgggtacgcg ttatggttat gaaatcgtgc ttgacggaaa
    10201 aacaccgacc ttgccgtatg ccaattcatt tcaaacgcag gctttatttg agtggcggac
    10261 cgatgcgccg gacttcacgc tggcgtttgg ttcatgcctt tatgtgaatg atccgccgta
    10321 tgatcgcccg ggccgtgctt atggcaatac atccgaaatt atgcgcgtaa tcggtaatgt
    10381 gaaacctgat cttatgttat ggatcggcga taacgtctat ttgcgcgaag tggactggaa
    10441 ttcacgcagc ggaattctct accgcaatcg ccatacccgc gcaatcaaag aactgcaacc
    10501 attgcttgga aatacgcata actatgctat ttgggacgac catgattttg gtccgaataa
    10561 ttccgatcgc agttatgctc acaaagacca tacgctcgac gcatttaagc tgtattgggc
    10621 caatccaaac tacggattat ctgaatcgcc gggcgttttt tttcaatttg aatggcagga
    10681 tattgatttt tggatgctgg atgaccggta tcatcgtgca ccggatgata tgatggatgg
    10741 tccggacaaa accatgctcg gcaaaacaca acttcagtgg ctcaaagacg gtttgctttt
    10801 tagcaatgca ccgttcaagg tgattgtttg cggtaatcag gtgctcaatg cgaacacgac
    10861 gtatgagagt tataattttt ataaaaatga gcgtgccgat cttctgaaat ttatcaaaga
    10921 taacggcatc acaggcgtcg tatttttgag cggtgacatt catcacagcg aacttcttaa
    10981 atgggaagat cccgattttt atacgctgta cgattttacg tcatcgccgc tgaccagcgg
    11041 catcagttcc ggcgatacgg taggaaccaa tccgcaattg gtggacggca ccaaaataac
    11101 ggatgcgcat acttttggcg ttatacgttt ttcggggaag cgtagagacc gtgtaatgac
    11161 tatggaaacc tgggatgaac acaataaaaa acgctggtcg ttttctattc atgaaaacga
    11221 tttgcatccc gcaaaaaaat aatttctttt tttaaaaata tctctttatc gagcgggaaa
    11281 aaagttgaga tgaaaaaata tattttagga tctatcgttt tatgcttgtg tgtcggcgcg
    11341 ctctacctgt ggaagggtaa cggtagcgac caaactaagc gcaacgcatc gggaagcgcg
    11401 ttggttatcc gtcctgccgc gttggagcgt ggacgactga atgtggatgt gtcggcgacc
    11461 ggcgttatca atccgatcaa tgtggtggaa gttaaatcca aagccagcgg attggtggaa
    11521 aaaattccca tcgaagcgag cgatgtcgtg cattccggtc aattgatcgc tcggcttgat
    11581 cagacggata cccgcaatgc gttggaacaa gctattgccg actcgcaagt tgcttcggcc
    11641 gtgctgatac agcaggaaaa taactggcgc cgcgctaaag atttgtttga gaaaaatttg
    11701 cttgcccaat ccgaatacga tcagatcaat gtcgagtatg tacgcgccaa agccacattg
    11761 gtcaaatccc aatccaatct tgttttagcc cgccaaaaaa tggcagaaac gaccgtacgt
    11821 tcaccgattg acggcattgt cctgtcgcgt accgtatcgg aaggtcagat cgtggcttcg
    11881 gctgtatcca atgcgggcgg aggaacgacg attgcccggc tcgcggcgat ggatcgcgtg
    11941 tatcttacgg ccgctgttga cgaagtagat attgggaaaa ttcgcgtggg tcagcgcggg
    12001 cgcatcgtgg cggatgctta tcccggcgat gtttttttcg gtgaagtggt tcgtgtggcg
    12061 gcacaatcta cggtcattca aaacgttaca acctttgatg tgatattgct cgtacccaat
    12121 cgcggaaaca aactcaaagc cggtatgaat gcgaccgtga ccgtagatat cgcagggaaa
    12181 gaccatgctt tgttgatagg caacgacttg ctgcgtacgt acgcggaggt gcaggcggat
    12241 aaaaaaattt tagaagaagc cggagtggta ttaccggaaa agcgaggcga tcgcacgcaa
    12301 gactccagcg ggcgcggtat gggccgtaag cgtatgcgtg aacaaaattc cggcagtgat
    12361 tcgacgcgtc tgcgttttgt cgtactttcc gaagccggaa cattgcgtgc gcatccggtg
    12421 cgcatcggtt tatccaatgc ggatgtttcg gaagtgttgt ccggattgga cgattcgtcg
    12481 cgcgtcatgt tggtcgtcta ttcacaagcg cttaaagaaa gcgaacgatt taaagatcgc
    12541 atgaaagagc gcacttcatt ttcgcgctga gtttgaacgt tttgtagtaa cattacccta
    12601 tcattcatca tgaatattct cgagagtctt cgcagcgcgc tttgggcttt gacgtccaat
    12661 cgattgcgta cgtttttgac gatgctcggc atcattatcg gtgtgggggc tgttatcaca
    12721 atggtggcga tcggtgaagg gtctcgccgc cggattcagg agagacttca agggctcggt
    12781 accaatcagc tattggtacg accgggttct caaaatgtcg gccgtacggt gcttgcggcg
    12841 ggcagttcgc aaacattgac ggaggaagat gcggagacca tattgaaaac ttcggaacat
    12901 gtgtcggctg tttctccgga attgtcccgt aatgcgcaag tcaaatatga atctaagaat
    12961 accaatacaa cgatcaccgg tacgcgcatc gaatttttga aagtgcgaaa ttttgttttg
    13021 gctgaaggtc gttatttctc cgccgaagaa atcaaaggaa acgtcaaagt tgctgtattg
    13081 ggccagaccg tggaagaaac actttttgga agtgcccaat cggtggggag tattatcaaa
    13141 atcaacggcc aaaattttac cgtgatcggt gtgcttgccg gtaaaggaca gaccggtatg
    13201 gggcagaatc aggacgatca gatcatggtt cccctgacga cagcacagac gcggcttttc
    13261 ggattggact ttgttacgac gatagcggtg caggtatatg acgaaaacat gctggatgag
    13321 acgacgtttg atattgaacg cgctctgcgc aaaacccaca aactgcgcgg tgatgcggaa
    13381 aatgacttca gcattcgcaa tcaagcggat attttggcga cggcgcaaga gacaagcgat
    13441 acgatggcga tacttttagc atcgattgca gcgatttcgc tcatcgtcgg cggtatcggt
    13501 atcatgaata ttatggttgt atccgtcacg gagcgcacca aagaaatcgg tattcgaaaa
    13561 gcgatcggcg ccaaacgcaa ggatattctc ctgcaatttc ttatcgaagc gatggtgatt
    13621 tgtttgtttg gcggattgat cggtgtcggt attggtatcg gctcggcgta tgttctgcag
    13681 tggaatgcag gatggcgcac agtcgttact ccggattccg tacttctgag tttcgggctt
    13741 tccgtactcg tgggtatttt tttcggattt tatcccgcgc tcaaagcctc gcaggccaat
    13801 gtgattgatg cgctacggta cgaatgaaat gaaaaaaaag tatttaactt ttattattca
    13861 acaacgtaca aaaggatcgt attaataata aatatcatgg agagcgtatg aaaaaactag
    13921 tcatgttatt gatgcttggg gtaggtttta atgtgtatgc cggcgataag ataccgggat
    13981 ttgtttacgg tgatgtcaag tcagtattga acgcggcacg tgaaaaaaaa caagtggtga
    14041 tgattgattt ttatacggcg tggtgcggac catgcaagct tttggaaaaa aatgtttatc
    14101 gcagcgcggc gtttttaccc gttaccgaaa aaatggtatg ctataaaata gatgcggaaa
    14161 aaggcgaagg tattgaactg gctaaaaaat acaacatcac cggttatccc tccgttgtat
    14221 ttatgtacgc cgacgggaaa gaaatcgaac gcaaagtagg ttacaatgaa gatatcgaag
    14281 gttatatagc cgatcttatg cgcatcgccg aagccaaaga tgtacttccg gtgtggatgg
    14341 ccgaatacaa tgcaaataaa aatctgacac tcggtgaaaa aattgtgcgt tattatttat
    14401 cggttgatcc gcgatcggct gaaccgttct ataccgaact tatggctatg ccagggatgt
    14461 cggacgaaca aagtgcggca ttaaaacagc agcacgctta tcgtggtttg gattatgggt
    14521 tggaaagcgc cgctaaagag gcggacgaaa ttattcgcac aagtaaggtt gaagatgatt
    14581 acgatctgat ttcgctggcg tttaactatg ccttttatct tacgcgccgc aatcagaaag
    14641 aaaaagcatg ggaaatagca agcgctctgt acgctaaact ggatgcggaa aaacggcaga
    14701 gttatgcgga aaattttatg gaaatcaaat accgttcggg tcaaacggac aaatcggaag
    14761 acgaaacgcg cctgcgcgga attgatttga gcgacgccaa cggtgtgtct gaagcggtaa
    14821 agattcacat aaaatggaaa gaaaaagagc gagccgaaaa tgtactaaaa acatggctgt
    14881 cgtcgtacgg taccaacgcc tctctggatg atatgaataa cgtcggctgg acggcgtttg
    14941 aagtcaaaat catgatgcgc gagtttgccg ctgcgatgat caaaacgtgg gacgaaactg
    15001 tacttgaata tcaggacgct tacaaagctg ataccatcgc caatctttgt gaagccgtcg
    15061 gcgataaagc aaatgccgta cgttttgggc aactcgccgt gaatatgatt cctgaacgct
    15121 cacgtatgcg aaaagaattt gaagacaacc taaaacgata tcaggccatg ccgtagaaaa
    15181 aataaatagt atttattcaa accgcgtatc caaaaaaatt acgcggtttt tttttcgcaa
    15241 aaaaaatgaa gtgcaaacag tcaaaaattt gatgttgaaa taatatgcgt accgtccaaa
    15301 ctcaacatat acaagcgttg gtaagattat aaacaatgtt attcacaatg atacaaacat
    15361 acgcttattc ttgttgataa actgtgaaaa aacttgactt tcggtttcga tcaatgtact
    15421 ttatcccgtt gtaatccaat agcttacgaa aataaacact gaatttttag gagttcgaat
    15481 gcctgctccc gccgatgtta agatttttga cgtcaaaaac actaaacttt tcataaacaa
    15541 tacgtttgtt gatgccgcat caaatcgaac atttgaaacg atcaatcctg caacgggtaa
    15601 agcgatatgc gctatttccg aaggcgataa aaacgatatt gatacggctg ttgcggccgc
    15661 gcgcaaagct ttttctgacg gaagcaagtg gtcgcgcatg agcggaagcc gtcgcggtaa
    15721 attgttgtgg aaaatcagcg aattgattac caaaaatgct gatgagttag cctaccttga
    15781 gactgtggat tgtggtaaac caatttccga aactaaaaac attgagatac ctgtaacggc
    15841 agatattttt gcatattacg cgggcgctgc caccaagatc gagggcgaaa caatacctgt
    15901 taacggtaat tattttaact atactctgcg tgagccgctc ggtgttttgg gattgatcat
    15961 tccgtggaat tttccgttga tcacggcagc acgcaagatc gctgcggctt tggcggcggg
    16021 caatacggtc gtagtgaagc cgtcggaatt tacaccgctc acgacgctca agctggccga
    16081 actgttcgcc gaagcaggtt ttcccgaagg cgtgtttaat gtcgtaaccg gatttggaaa
    16141 aacggccggt gccgcactgg tcgcgcatgc cgatgtggac ggcattgcct ttaccgggag
    16201 tacgacgacg gggcaggaaa ttatgaaaac tgcgtcgttg acgatgaaac gtctttcgct
    16261 ggagttgggc ggcaaatcac cgaatatcgt tttcgcagat acggatctgg atacgacgat
    16321 caagatggcc gccgccgcta ttttttacaa caaaggtgaa gtctgtacgg ccggttcccg
    16381 tcttctgatc gaagaaagta ttcatgatga atttatagaa aaattagctg cccgtgcagc
    16441 caaaatgatg ccgggtgatc cgttgaatcc cgagaccaaa ctcggtccgc tcacatcgga
    16501 actgcaaatg aataaagtga tgagttatgt gcaagccggt aaatcggaag gtgcggcgct
    16561 tcttgccgga ggaaatcgta tcggcaacga cggatacttt ttccaaccca ccgttttcgg
    16621 aaatgtggat acgtccatga aaattgctac cgaagaaatt ttcggaccgg ttctcgcgac
    16681 gatgaagttc aaagattttg atgatgtcgt cgaaaaagcc aatcgcacgt attacggact
    16741 tgccgcaggg atatggacgc gcgatatcaa gaaagcgcac acattggcca aaaaaatcaa
    16801 agccggcacg gtatggatca atacatataa catgtacgat gcggcgatgc catacggcgg
    16861 atacaaaatg tccggtttta cgcgcgaatg cggtatggaa gcgatttacg aattttatac
    16921 gcaggtcaaa tccgtatggg tggatctcaa ttgaaaaaag agtttttata tatttcggtt
    16981 ctgatgtgga gttttgtcgt ttcgggttgg gcgcaaaagg aaacaccgaa agctgcgccg
    17041 aagtcttacg tagcggctgt ttttaccatc gggaaaaact gggatacgac caaaacggcc
    17101 tacgagcagc cgcacttcaa agaacacgga gccaatctca aacgcttgca tgcggaaggt
    17161 aagatcgcgc ttggagcacg gtatggcgaa tttggtcttg ttgtatttaa aacagaaaat
    17221 atggaagaag tgcgtgcctg ttttgaccaa gactccatag tgatcaaaga tattttgcgg
    17281 atggatattc aaccgtttcg tccgttttac aaaggttgta ttgactgaga aataaacaca
    17341 ttgaatgtag atacagagat aaggaatggc atgattatca gtaagttttt ctttcgtcgt
    17401 tttttttggg gtgcagtggc cgtgtcggtt attttgaccg gatcactatc ggcgcagaca
    17461 gggcgtcagt taattgacaa aattgccgcg gtcgtggacg atgaagcgat catgatgtcg
    17521 gaagtgcaac agtatgcgta cttcgaagcg atgaacctac acatcaatcc gagtaatacc
    17581 gaagaatttg ataaaatcat gaagcgcgta ttagagacca tgattcagca aaagatcatg
    17641 ctcgcacagg cgcggtttga cagtatcacg atagatgaag cccaagtcga acaatcactg
    17701 gaacagcaga ttcgcgagcg cgtccagcaa gccggcgggg aagacgaact cgaacgggcc
    17761 atgggcaaat ccgtgaaaga acttaaacgc ttgtaccgac cgaatattcg taaacagatg
    17821 ctggcccaac gcatccaaag cactaagttt aacgatgtca aggtgtcgcg cagcgaaata
    17881 gaaaaattct ttgaagagtt tcaagacagc ttgcctcaga tcggagcatc ggtaaaattc
    17941 agccaaattt tacttgaggt caaagccggc gatgaagcgt ttgccgaagc gcgacgcatg
    18001 gcgcaaatgt atcttgactc gatcaaaaaa ggtgcagact tcgcaacgat cgccaaggcg
    18061 gtttccaccg atccgggttc cggaaaaaaa ggcggtgatt tgggctggtt caatcgcaac
    18121 gactttgtaa aagaatttgc cgatgcggct gtgcgtcttg aaccgggtga aatttccgat
    18181 gtcgtcaaat cggaatttgg ttatcacatc atacagatga ttgaaaaaag cggtgaacgc
    18241 attcacgtgc gccacatttt attgggcgtg gccaatggtc ccgaagataa aacccgtacg
    18301 cgtgaaaaat tgggccgcat tcgtcaggat atcttggaag gcaaaatatc ttttgaaaaa
    18361 gccgcactgc agtattcgga cgatccggcc aaaaaaggca atctcggtaa catgggatgg
    18421 atagagatcg ccacgttgca ggataaatcg tttgtaaaag cgctggatcc tctaaaaaac
    18481 gaagaaatct ccgaaccgtt tgaaacgcca tttggtttct tcatagtaca gttgcatgac
    18541 cgtcgtgata cacgcaagtt gaatcttaat gaagactggc aaaccattga aaatttcgcc
    18601 ctgcgtcaaa agcaggccaa agaaatggac aaatggctcg aaaaaatcaa atccaagttc
    18661 tttattgatg ttcgtttata gaatgttttt gtaaaagaat atgatggaat ttatatcggc
    18721 acagtggttt ttatggcgtt cgcggttcct caaaccgacg gaggtgatgg attttccgca
    18781 gcgtttatct gagatcaaac gtgtgttggt atattttccg atggccatcg ataaatcacg
    18841 cgccgaaacc atttttggcc aaatacaaaa aacactgtcc ggttgtacat tggatttttt
    18901 gctacatccg gaaacgccgc aagaagtttc cgcatttctg accgaacggc aagcgaccgt
    18961 aacgatcttg gccaagaccg attttggttt gttacggaca ttaaacggag aagccgtaga
    19021 tcgtattcgc gcaaaagaat acgatatgat ccttgatttt acttttacgg ccgactgggc
    19081 gatggcctgg gcactccgtg cctgtggcgt atccgtcctg gccggcgcat ttaccgaacc
    19141 gcgcagcgaa cgctttcaca acctgctgat caaagcccac gatgcgccta cctttgacgc
    19201 gacgctgttc ggatgtttag cacagatgaa aacggtgatt tagttttcag ataacctgcc
    19261 ttttggttgt atatatgagt cagcgcgcaa aacaatggac gtaccatctt tccgtggccg
    19321 ttttagcgat cggcatggtg gtggtgttta attttttggt atgccatccg gtggattttg
    19381 ttcgtaccaa tacgaagcca gccgagtgga aaacttatgc caatacaaaa ttcggttttt
    19441 cagttgaaat tccttcgacc tggagtattc gtgaagatgc acagggcgtt gaactatatg
    19501 atgcggaaac gcgtcgctat atttcagtaa aagcgttggc cgaggagtcg tcccttctca
    19561 atatcccgac cgatgaatat acgattgaac gttcggtctt gcgtcatact tacgtgtgtg
    19621 acacgatacg tgaaccgcac cgggtggtga ccaagtcggg gttgaccggc tatatgatcg
    19681 caccaccttt gcgcaacgat gttgaccgga gtcagatcaa tgcgccgtgg gcagatcgtt
    19741 gtgacgcttc cacgttatcc ttgacttatt ttcccgtgca atttgccggc gatatctatg
    19801 cttcttttgt cgaattttta tcaaccggtc cgtcggacgc ggtgtatgaa catatgaccc
    19861 aatcataccg gcatttattt aaaaccctcc ggcagggcga attaatgcga atgcacctca
    19921 ttgatacatc cgagacacct tcgccggctg tagcgtacgt aggcgaaacg cgcgggtttt
    19981 atgtggattt tgatgcggat ggcacggacg aattacttat cgccggtatg ccgcgtaata
    20041 tggaagatac caaagagcga tgctttttcc gcgttctaaa aaaacaaggg gatcaatggg
    20101 tttgggcttt tgaacgcaaa tactccgaaa attcgttcca cagttcggac atcaaaattg
    20161 tcaatttgga taaccgattt ggtccggatg tatttttgcg ttttgtcgag catgggaacc
    20221 cttggggaaa aaacagtacg gtgattattt tttgggatgg ggacaaattt cgcggcgcag
    20281 actttggcgc attcggcgat gcacgggacc ttaacggtga cggtaccgat gaagtgattc
    20341 atacgacgaa ttcatacttc agccaaggtg cgattgcctc ctggtatgat gtatatacct
    20401 atgacaaagg tgagtttatc gaggtcaaca gccgcttccc tgaattttat aaaaacacgg
    20461 ttttacccaa ataccgcgat caatatgaaa cggtgcaggg tgaattggct ctgacgcggg
    20521 tggaacaatt caagggcccg gcacgtcaac ttatgtttag attgagccgt tacattgata
    20581 tgtcaacgga tattagcgaa ggtcgccctg cgcgagaacg ctggtaagaa acggatcatt
    20641 ttttcttctc acctatttta atattccgct aaagtatgag tatgtcattt agggatatcg
    20701 ttgtgctctt tgaggacgaa cacttcattg cagtacataa accgtccggg cttttgacga
    20761 ttgcagaccg gtttgatacg aaagcgccga atttagcgga tttactccgt gatgaaaggg
    20821 gtttcgtatt caaagtgcat cgcctggata aaccgaccag cggtgtagta cttttcgcta
    20881 aacataagga agcgcaccaa tccgtcagtt tacaatttga aaaacatacc atccaaaaaa
    20941 attatttggc attagtttcc ggccgcgtac aggaaaaaca atacaacgtg gatgtgccga
    21001 tcggtgaagc ggagcgcgga ttgatgaaaa ttgatcgtac gcatgggaaa ccttcagaaa
    21061 cagagtttag cgtgatagag tcgtttacgc gatggacatg gcttgaagcg cgtccaaaaa
    21121 cagggcgtac ccatcagata cgaatccact gtcttgcgaa aggtacgccg atcgtcgggg
    21181 atatgcatta cggtagtgag accggaatat ttttatcgca gttgaaaaaa aactataaga
    21241 gaaccggcga ttcggatgaa aaagccttgc cgggaagatt ggcgcttcac gctgcagcgc
    21301 ttactttttt gcatccatgg gaaaacaggc caattactgt ttcagcgtct ttgcccaaag
    21361 acctgcaaag tacattgaat cagcttcgaa aatatgcggc tgttgcggcg tcttcacacc
    21421 atgaaagcgg agcgctgatg atgtgatgcg atcgcatcgg cttgccacgc gccatctgtc
    21481 caatctatcg aaaaaaattc agaaaaagct tcccggagcg attctttgag agcagcaata
    21541 tccacggcta tgtactgatt cagctcggta gttttttcat tcataacgcg ttgcaaaata
    21601 tttttagtag ccgcgtcggc atggatataa tcggccagct ttcggtgaaa atctccgatt
    21661 aggatcgaac catgctgcag cgtagcctgc gcatatcgcc gctgcgcact gccaacgact
    21721 ttttttccct caatctgaat ttctgattta gcggaactgg agaaacacaa cgcggaagag
    21781 ggatttcggt agtgctgctt gaaatcagtc tgctcggtcg tacaataggc ttcatatccc
    21841 aatcggcgca aagcaatagc aatcgccgtg ctgatatgcg cgtgggattc ttcgaccgaa
    21901 ccgcggatat cgtcatgata gacaatactg taggtcaatt cttgtgcatg gagtatggca
    21961 cgcccacccg tcggccgaaa gacaatttca agtccgtcgt gatgcgcttt ttcggaattg
    22021 atttcggata tattctgatg tttgcccagt gaaatgcaat acggttgcca ttggaaaaaa
    22081 cgtaaagtgg tcggccgcaa accggagccg acttctgccg ctaaatattc gtcaaaagcc
    22141 atgttgaaac ttccgggctg cttccccgta tctatccact gccatgtttt acgcacagaa
    22201 ataacttgat tgttgaacgc ttttagagta atatgccacc aagatagcca acaccggtta
    22261 tccaaacaat gtttatctta gccatgcgtt ttatacctaa catattaact atccttcgca
    22321 tcctgctaac tccggtattt gttttttgtt tgctgtccga tgagtatcag gtttactccg
    22381 ttgctctttt tgtttttgca tcggtgaccg attgggtaga tggttatttg gcgcgcaaat
    22441 acagttacca ttctggattg ggtaaattcc ttgatccttt ggctgataaa atactggtat
    22501 tatcggcatt tttttcattc gtttatctcg gatttgtaaa gctatggatg gtgatggtga
    22561 ttgtgatccg cgattttatg ataaccagcc tccgttcgta tgcgatacgt cgcggtaaac
    22621 cgatgatgac taattttttt gccaagctca aaacggcctt tcagatgttg atgatcggat
    22681 tgattttaat ttttattgcc atgcagtcgt tgatacatat gacgatcagt gagcagttaa
    22741 attatttcat ctacatcgtc acgcactacg aagtgatcta tgtaggcatg ctggtggtga
    22801 cggcggtcac cgtgatatcc ggtcttttgt atttgtatga aaaccgtgaa agccttcgtt
    22861 ctcttcattc accttcacaa gtcaaaaaca cgtaaatccg atgctgaatg cgcataagct
    22921 cctgggtacg gcgttggggg tgggttatct tccgaaagcg ccgggtactt ggggaagtct
    22981 tttggcactg gctatatact tcattttttt tccttcgtca tcttccttat tgattcacgc
    23041 tgtttttttt attattatcg ccgcagtttt cgggcttggc gtggtctccg cgacgcgtct
    23101 ggaaaaggtg tacgggcacg atccttcgtg tgttgtcatt gatgaagtcg ttggactttg
    23161 gatcgcctta tggtttcttc cgaaaacgtg gattgtgatg atcggcgctt ttgttctttt
    23221 ccgttttttt gacattacca aatggtttgg cattaatcgc ctgcaggact taccgaaggg
    23281 atggggcgtc atgatggatg atgtacttgc cggtatttgg gccaatgtaa ttttgcaaat
    23341 agtcgtaaga atatattgat caatacagct ttgaggagaa tggtatgagt gttttggtag
    23401 ataaaaagac ccgtcttgtg gtacaaggta ttacgggtaa agaaggtact tttcatacaa
    23461 gtcagatgac agagtatggt ccgtacgtag tcgccggtgt gacaccgggc aagggtggtc
    23521 ttgtatataa aggaaatgaa aaagataaac tgaagtatgg tgtacccgtt tttaactcgg
    23581 tcaaagatgc ggtggaaaaa gccaaggcca atacatcggt gatctttgtt cctccggctt
    23641 ttgctgccga tgccataatc gaagcagccg aagcaggtat caaagtgatt atttgtatta
    23701 ccgaaggaat ccctgccgcg gatatggtgc gtgcctacaa tttcatcaaa gataaaggtg
    23761 tgcatctcgt cgggccgaat tgccccggtg tgatttcgcc cggtaaatgt aaagtcggta
    23821 tcatgccggc atttattcac aaacccggtc gcgtcggagt tatttcccgt tccggaacac
    23881 tgacctatga agcggtggga cagctaacgg cattgaaaat cggtcaatcc acctgtatcg
    23941 gtatcggcgg tgatcctgtg atcggtacgc gtttcgtgga tgcattcaaa ctatttgccg
    24001 aagataaaga tacggatgcg atcattatga tcggtgaaat cggcggtacg gctgaagaag
    24061 aagccgccgc atgggttaaa aagaatttca aaaaacccgt ggttgcattt atcgcgggcc
    24121 agaccgcgcc tccgggtcgt cgtatgggcc atgccggtgc gatcatcagc ggcggcaaag
    24181 gtacagcgga agaaaaaatg aaagcgctga agtcggctgg aattcacgtc gtcaaaagcc
    24241 ctgccgatct cggcgtgacc gtagcgcagg tgcttggcat taaagtaaaa agtacgact
//
LOCUS       DAOQNQ010000018        23651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_730_length_23651_cov_5.742244, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000018 DAOQNQ010000000
VERSION     DAOQNQ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 23651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23651
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_730_length_23651_cov_5.742244"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PK754_02405"
     CDS             complement(<1..260)
                     /locus_tag="PK754_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08385.1"
                     /translation="MKIISESWFKRHRWTLLVFLFLVFRPHITAAIRNHVVIPFKKNI
                     LHDHRTMPSKKLFKPKAELRVHYAGIVLLNDTGDTEDDGCSD"
     gene            475..2058
                     /gene="pruA"
                     /locus_tag="PK754_02410"
     CDS             475..2058
                     /gene="pruA"
                     /locus_tag="PK754_02410"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HNB08386.1"
                     /translation="MATARKKKTFKYSVFKNEPFTDFSKPANRKSFEKALEKVSKELG
                     KEYHIIIGGDRIRTNHKMESRNPSNREQLIGIFYDGNEELANKAVETAAEAFKKWSRV
                     APEKRASYLFKAASIMRKRKHEFSAMMVYEVGKSWAEADGDTAEAIDFMEFYGREVLR
                     YASEQPLTKIAGEDNQLMYIPLGVGAVIPPWNFPLAILVGMTTAAIVSGNTVVLKPSS
                     DSPTIAAKFVALMEEVGVPKGVINFLPGPGSGMGDAITKHALTRFIAFTGSKTVGLHI
                     NKVAAETQPGQLWIKRVIAEMGGKDSIIVDNEADLDKAVDGVLYSAFGFSGQKCSACS
                     RAIVVESVHKKFTEKLIAKAKTLTVGNPADPKNYMGPVINDKALGKMMWYIDKAKQEG
                     AELLLGGVKDDMQGSFLMPTIFDNVKPKDTISQEEIFGPVLAIIKAKNFDEALEIANN
                     TEYGLTGAIFTKSRKKLERARNEFHAGNLYLNRKCTGALVGVHPFGGFNMSGTDSKAG
                     GRDYLMLFTQAKVVSEKLK"
     gene            2128..5592
                     /locus_tag="PK754_02415"
     CDS             2128..5592
                     /locus_tag="PK754_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08387.1"
                     /translation="MHDTDPLYRSLQIPLTRLRREENRLYFQTNALRGAAFLLLLALL
                     TASIEVLSDPDVLGRWVLAMSALMIACVIIAFYIVSPILRLFGVVAASSDFVLAQQIG
                     QRFPEIKDRLLNALQLFGELREGNEKVSEPMIIAAIQTIHTTLEKYDIISVADRTVLR
                     KTWRYTSVVVIAASIVFLFFNRPLTDAFARLADPGKKPMEVIPYRWVIVPGTTDILQG
                     QNRKISVEIIPLTSDAAMPDAVQLLTQLSGVEQVTETLMKKDTARFYSLELQNLRQET
                     TYWFKFSYVENSRKRAAISEKNTLHVMRRPSVKRLQIEIESPTYARIEHRILEANSGD
                     IQALKGSRVRVTMEATKSLQEARIVFSDSTSIPLKTSVFNYEKAEGSFKVMKSGTYHF
                     VMTDKEGTPSADPVEYRIDVQDDLYPLVQLNIPEKDGDITEAMLQKIVADVQDDFGVS
                     RIVLNYKLMHTRGVLPIPETYTTEDLSFLIRSDQSEFDVAYNWEFSKLLLQPEDVIGF
                     YLEVWDNDAVSGPKKTKSVERRLRFPSMEEIFAETNQKQDQQIQAAEEIAKESEEIKK
                     SLEEVHREMLKDKKPEWKDKEKIKQLVEKQEELQKKAESIQKSLEEMTDKLKEQNLLT
                     PETVQKYQELQKLLSEINSKEMQELMQKMQKAMENNFDPNQMRDALKNFKMDQEAFKK
                     NVDRTVELLKRIKAEQMFDQLRRTAEDLEKQQKDLDRMTSNLKNDNERTAAIKEQERI
                     RKNTDDLKKKSDELQKLVKELNNNQSNQKLNTAQDQLQQNNIQNRMQQSQQAMSDKQE
                     GTKEEKENREDIHKSLDSLSRSMADAQKEHRANQDEAIKRAMQKIVVDMLVISQNQEA
                     LISETSPLNFASVRFREVTQDQSDILSNMGIVTENLLELSNKTFFVNTPLGKTVGQAM
                     GQMQTAVKAMEERNTPQAVNSQTAAMVSVNEAIKLLLLSMDKMNNGQSGTGMEELMEQ
                     MREAAGKQRRINQGMPSLMEGQDNNPGGLSQEQQAQLGRMMAEQQALKQSMEAMKDQA
                     GAQQGLKGQLGNLAKEMEEVIKDMQSNQVNRKTIERQQKILQRMLDASTSMQEKDMSE
                     KRKGETGKNYDAVSPRALPENLTDRRNFLRQELLRQMKQGYAKDYQELIKRYFDELGE
                     KTGLPKND"
     gene            complement(5589..7235)
                     /gene="hutU"
                     /locus_tag="PK754_02420"
     CDS             complement(5589..7235)
                     /gene="hutU"
                     /locus_tag="PK754_02420"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNB08388.1"
                     /translation="MRTIRAPRGTSITCKNWLTEAPLRMLMNNLDPDVAERPEDLVVY
                     GGIGKAARNWDCFDAIVKSLTNLAMDETLLVQSGKPVAVFKTHADAPRVIIANSNIVP
                     HWATWENFREYEKLGLIMYGQMTAGSWIYIGTQGILQGTYETFAECGRKHFKGTLRGT
                     ITLTAGLGGMGGAQPLAVTMNDGVAICIEVDRTRIQRRLDTRYCDVMVESLDEAWRMA
                     TECKAKGEAKSIGLLGNAAELLPEMLKRNMIPDIVTDQTSAHDELNGYIPTGISVEEA
                     TEWRKNRTQEYIGRAMESMAIHVRAMLDFQSRGAIVFDYGNNIRGQALKVGVTNAFDF
                     PGFVPAFIRPLFCEGQGPFRWAALSGDPEDIYVTDKAIMEAFPEKAGLQRWLSQAKDR
                     IAFQGLPSRICWLGYGEREKAGLIFNELVRTKKVKAPIVIGRDHLDCGSVASPNRETE
                     AMKDGSDAIADWPLLNFAINAVNGATWVSLHHGGGVGIGYSLHAGMVIVADGSPEAAV
                     RLKRVLTCDPGMGVVRHVDAGYDEAIKVAKEKNVHIPMMR"
     gene            complement(7260..8243)
                     /locus_tag="PK754_02425"
     CDS             complement(7260..8243)
                     /locus_tag="PK754_02425"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNB08389.1"
                     /translation="MVNETAFTRHAGVQYPILCGAMYPCSNPELVAAVSAAGGLGIIQ
                     PLSLTYVHKYDFRQGLRYIRSLTDKPIALNATVEKSSRVYEDRLRQWIDIALEEGIRF
                     FDTSLGNPRWVVDRVAPYQGIVYHKVTERKWAEKAMDAGVHGLIAVNARAGGHAGHLS
                     PQQLYDALADTGLPIVCAGGIGDENDYADALKIGYAGVMMATRFIATTECTAHEDYKR
                     AIVQAGEADIVHTERVTGVPLAVIKTEYVERVGYKTGTIARLMLKHRQLKKMMRLIYA
                     LRSMRQLKRSSLQGKMSTRDYYQAGKSVTGVHAVESVAAIIARFVRSTKPK"
     gene            complement(8233..9165)
                     /locus_tag="PK754_02430"
     CDS             complement(8233..9165)
                     /locus_tag="PK754_02430"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HNB08390.1"
                     /translation="MRFASIDIGTNTVLLLIAEADSSGHLHTLFEKQRIIRLGKNVDA
                     QKNIAHEGMDKLTHVLTEYLQDAANHQTAKIVIGATSAMRDAANKETILNEIYHRTGQ
                     RIQILTGDEEAQWTFQGGCLALPPLTRHPILLIDIGGGSTEFVVGSASDMTFKKSLNI
                     GAVRMTERFINHDPPLQSELESLRRYAHNEFDTHLSGIKNIQPAHALGVAGTVTTLCA
                     MLQALPHYDPKRINGYMIDKAALDSLIEKLSDLTLEERRKIPGLEPERADVIIAGATI
                     LSEIFSFFNLDKITVSNFGLRYGLILRELANHGQ"
     gene            complement(9165..10067)
                     /gene="prmA"
                     /locus_tag="PK754_02435"
     CDS             complement(9165..10067)
                     /gene="prmA"
                     /locus_tag="PK754_02435"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNB08391.1"
                     /translation="MQPKKKKSRSSTRTKKKKDFWWEVDLGHIVNLEATQTLLFELGS
                     TGSIETATGLTAYFQPADYSSEKILRAKLAPRLGPGTTVHIRPMAAQDWESEWKKNFK
                     PRRVGKHFIVRPSWEKYKRKLKDIILVIDPKMSFGTGTHETTQLVLGFIESFSVKNKI
                     LLDVGSGTGILAIAAAKSGARAVYGFDVDTNAFENAEENIRRNRVSARVKTVLTTIET
                     LPSKWPERYDIILANIQRAVFEMPGFMENLNQKMKPTGMMILSGILAIEDERMRMLFS
                     EHNLHVIEFRADGEWVAYVLQRTS"
     gene            complement(10092..11153)
                     /locus_tag="PK754_02440"
     CDS             complement(10092..11153)
                     /locus_tag="PK754_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB08392.1"
                     /translation="MKDHILVVDDELDAVDPLIRVLQHDGYEVDYAANAKEALGYVKT
                     TSYQLILMDVMMPGMSGYQLLKKLQEDEETAYIPVIFVTGHFNAEEIVKGLEAGAVDA
                     ISKPFHIAEVITRGKVRIAESKLKRRYTPIHHFFAEAQEKEQSRRTGCFEFFDKSKNK
                     VGDVFVSEGKIVYATSKNAIKEDAFLQLASMKDITYIFQEGVRSQNTSFSASVTSLIL
                     EASKIVDELEAKEIRDEDQKRVLVIDRDRIPRILASRALKSAGYAPTVTSPDEVTQDI
                     ISKYDPHVVIVDPQDCELVLNKVALTRPSRQPIPLIVYGDQDQLEKHDALRKYHIKAF
                     VDKAQTNESLAHAVFQALK"
     gene            complement(11191..11925)
                     /locus_tag="PK754_02445"
     CDS             complement(11191..11925)
                     /locus_tag="PK754_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB08393.1"
                     /translation="MAETKKTRSKLLLVDADEQARLLIKENLIKEDYIVFDTGNGNVA
                     LEIVNREKPDLVISDIFLGEMNGFELCQRIRDTSNVPTVPFVFFTAIDDMLTEIRGFR
                     CGANDYLVKRHTKRQDLLLRIESMVSQVNAFEKEKATLRDGFLGKLGDVSVIDVLQLL
                     ANTAKTGNLLISDGTIEGNVYFMKGQIFNATLGHVQGEDAIYDMIYWEDGFFRFTQEE
                     IPRQSVIASPTNKIISKCRRLLGKEE"
     gene            complement(11946..12668)
                     /locus_tag="PK754_02450"
     CDS             complement(11946..12668)
                     /locus_tag="PK754_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB08394.1"
                     /translation="MPKKILIVDDNKTVRTLLSINLRKENFEVIEAENGAEGLQMVND
                     HLPDLVISDIIMPQMDGFEFCRKVRETSKSPMVPFMFLTSIDQVATELRGLRTGADDY
                     LIKSNIKKEELVGKVSAMLQKSNEYKQAENQMQEGLYGKFSDLSLVDVFQLLIMNKKT
                     GLLTITRDSDKAEITFEDGRMLHAEYKQFVGEEAVYNLGEWKTGVFKFESVEPKVTPT
                     IHSATMNILMEYCRITDENKLN"
     gene            complement(12702..13925)
                     /locus_tag="PK754_02455"
     CDS             complement(12702..13925)
                     /locus_tag="PK754_02455"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNB08395.1"
                     /translation="MMRGFEKAADILELDTGVRQYLLTPEKQVTVSIPVVLEDGSIQV
                     FKGFRVIHSTVRGPSKGGIRVSPKATLDETTALAAWMTWKCAVVNIPFGGAKGAIECD
                     PSRLTESAYEKIIRRYTANLFEIFGPDKDVPAPDNNTGEREMGWIMDTYSMHVRRTET
                     AVVTGKPLIMGGSHGRRDATGRGIMLIALAAMKQRNIPANEARIVIQGFGSVGATAAQ
                     LLYENGCKIIGIGDASGSYFSEKGINISDAIRFAQQNRRSLSGYPNAQKITNEELLML
                     DCDVLIPAAMENQIHAENASKIQAKIICEGANGPITPEADTLLHERGILVVPDILANA
                     GGVTVSYFEWVQNRMGFFWPLHEVHDRLERVMIESFESVHAVALRHTQSMRIGAYILA
                     LDRVTQVLKLRGIYA"
     gene            complement(14103..15098)
                     /locus_tag="PK754_02460"
     CDS             complement(14103..15098)
                     /locus_tag="PK754_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08396.1"
                     /translation="MCCIVCATNPLRTILMFWMAIGLYCSGFPLYTQDRYDPEINRRP
                     YVPDVTKHSANTLDSITAADYAKRNKIKSNMADYKPSYWHINFIFGASYLHERNNIEQ
                     FYDNEGYVYHVADLKDPDEKKHNPKLIQISVQHNLSPHWQIEAVVSRIFNQTLAGSNW
                     LQFVNEEDTPPSQRFDLEQKSEIKMLQVGGRYIFMPVNMTTRLELSTGAGIAYYSYHT
                     SSHHYYAQNIGYHSHPQLINQTSSSFSYDRNALGAVADLSLDFYLSPKISCQFRYEKR
                     ITEKIKLPSESFEYVIRKQIGPREFIMETHEKHIPSRTIDLSGYTLNMALHLHLF"
     gene            complement(15141..15764)
                     /locus_tag="PK754_02465"
     CDS             complement(15141..15764)
                     /locus_tag="PK754_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNB08397.1"
                     /translation="MSKKPITNKDVTPRMFESDFLEFFSKVHFSVPLIVFIPIISYFI
                     YQSLWIEKSDIKHFLFWILAGLAVWTLTEYVLHRFIFHFTPRSALMERIHFIFHGVHH
                     DYPNDSKRLVMPPAASIPLAALFYFLFLIIFGSNIAPMFAGFLIGYLWYDMTHYAIHH
                     FNMHSAFWLAIKNHHMKHHFQNPEKGFGVSTPIWDYVWQTMFPAKNK"
     gene            15995..16495
                     /locus_tag="PK754_02470"
     CDS             15995..16495
                     /locus_tag="PK754_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNB08398.1"
                     /translation="MFETHIIEYSAKSEMYAALHAQIHHLLDGETDLIANAANASSLL
                     FHTLPALNWAGFYFYKGNELIVGPFQGKPACVHIAMGKGVCGTAAQQRKTVVVKDVHT
                     FPGHIACDSASNSEIVVPLISGGQLFGVLDLDSPQMARFDEADQAGLEEFVDIFMKHT
                     TWPKNL"
     gene            complement(16490..17041)
                     /locus_tag="PK754_02475"
     CDS             complement(16490..17041)
                     /locus_tag="PK754_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08399.1"
                     /translation="MATAMTKSEIIKMLTGNDRRTIAHANLLVSKLTHDRTGLKNLYH
                     LLSHEDADICFRASDTLEKILRNETAAIRTLSPYLNKDWPKLIGNEALQMHLPLILGY
                     VAWKKNDATRIGTRLLELAHTTKNKFVTVNTMTGLADIALRHTWLVPTVWGLLDEMEM
                     KGSAALRARARILKKKINLATKL"
     gene            complement(17022..18125)
                     /locus_tag="PK754_02480"
     CDS             complement(17022..18125)
                     /locus_tag="PK754_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004156379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNB08400.1"
                     /translation="MAEPLKNMFSGSFVETLGDQIQTVYSSFDKRAFTNFIFDHAWSQ
                     RELKDRMHHISSALRSHLPEDYRKALGILIHVTKHFRMNDTKISFTDMVFPDFVEHYG
                     LDDLESSIPALELFTQVSSAEFAVRPFILRYPDIMLQQMLVWSRHSSPHVRRLASEGC
                     RPRLPWAIALPVFQKDPAPILPILENLKADPSEYVRRSVANNLNDITKDNPEIVLQIA
                     ERWRGLSPETDKLVKHACRTLLKRGNKKALTIFGTHDASGVSVLSFVSKKKKIFIGDT
                     LDFETEIRIRKAKTLRIEYAIDFVKSGGKISRKVFKWREGLYDSGSLKLSKKHRFQNF
                     TTRKHYSGKHAITLIINGHPIADLDITLGDSHD"
     gene            complement(18129..18506)
                     /locus_tag="PK754_02485"
     CDS             complement(18129..18506)
                     /locus_tag="PK754_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018128486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB08401.1"
                     /translation="MNTKILGLCSVVQHVTDLEAAKRWYTDAFGVKPYFDQPFYVGFQ
                     FGGFELGLDPDPKQYNSPGFGSITYWRTDNVQAMFDHLIRSGAEANEHPNCVGGDLII
                     ASVRDPFGNVIGLMYNPEFKITA"
     gene            complement(18509..19039)
                     /locus_tag="PK754_02490"
     CDS             complement(18509..19039)
                     /locus_tag="PK754_02490"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB08402.1"
                     /translation="MNITAHLHQFESIANDWMMALDTYDERKFNRAPDQESWSIGQVY
                     YHLVHGTQNFHLRMVRQCMDNPDHAHEKKTFPGKIVFFTGRFPPIRIKVPPSDMYTPK
                     QPESIAAMRRDLNTLIGTMRTVASELSQKRNQGKAQHPRFGYLSGDEWFAMIQMHFRH
                     HLYQKNRLDNFLKKGA"
     gene            complement(19079..19420)
                     /locus_tag="PK754_02495"
     CDS             complement(19079..19420)
                     /locus_tag="PK754_02495"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNB08403.1"
                     /translation="MLILTLNACQANISSPGNDYITEVAVIKIKPEFVSRMPDIVAEA
                     KSIIGTYEGFISWENMRADDTSRVFVDILRWKTKDHADQAFEKFHSTESCSRLMNALE
                     KDVYFGHVRHY"
     gene            19640..21007
                     /locus_tag="PK754_02500"
     CDS             19640..21007
                     /locus_tag="PK754_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNB08404.1"
                     /translation="MHSDKMRVALTSVLAAIFLTGMKLAAGLATGSLGILSEAAHSAL
                     DLGAALLTYVAVRISDKPADDDHSYGHGKVENISALLQTGLLILTCFFIIRAAVERLM
                     SGDVQVEVNVWSFVVIIVSIIVDYSRSRALARIAKTYRSQALEADALHFSSDIWSSSV
                     VIIGLISTALGFHQGDSLAAIGVALFVLVISGKLLKETIDALTDSMPKDIEQRIRTAL
                     VSLDGVERYRHLRVRQSGSKVFVDMYIDVKRTVPFEMAHHVADAVETRIHEIVPNADV
                     IIHMEPQETDDESIIDKIRMIVVQEGLACHNVRAQRVTNGYFVDFHLECESHQRFDEA
                     HEASSRIESRLMEKIPEIKDVKVHIEDARDRVVQAFDITDQSKPLIAAVRAIAANTQP
                     VRACSDFIVMDIGGHKKLMLNCVVPGDLSLDEVHAITTRLENRIHRDIPEVSSIVIHP
                     ELARA"
     gene            21000..21773
                     /gene="pgeF"
                     /locus_tag="PK754_02505"
     CDS             21000..21773
                     /gene="pgeF"
                     /locus_tag="PK754_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNB08405.1"
                     /translation="MPEPSMIFEEKDGLRLCRFPALEKFPDVMAVMSTRIGGVSEGDY
                     KGLNLGLSTDDNPHHVEENRQRFWRALGVDMNDVIRQHQVHEDRVVFLQEQTLCADTD
                     AMYTDQSKKFLTVVAADCVPVLFYEPAHRIIAVIHAGWRGTLKQIIKKTMASIQQTFG
                     CSLGHAVAVLGPSIGADHYEVSDEVASMFDTAFVNRRDYPKPHLDLWKANAAQLDEAG
                     VGHIVVTGACTMCQPDIYYSHRASGGRSGRMVGVIGRRN"
     gene            21778..22500
                     /locus_tag="PK754_02510"
     CDS             21778..22500
                     /locus_tag="PK754_02510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB08406.1"
                     /translation="MDHIEKYFGSLIQRAGDAIFTVSPSQIILSWNDGARELLGYDKE
                     DVLGHSVDVLSPLDDKRHMRAMAIEVFDHGALKNLETELTRKDGHVVSAYVTVSPVTD
                     ENENVVAVSFIAKDITDQNRLLIHLIESQRQAGQIQALVEALTTLSHHIRNASAVISA
                     KSEVSLELNQLAVYHDLVRVCTHETRRITAVIDTLQDLVREMQVSGEPIDTVQMNGAP
                     APLLDIEARLKERLKKLDERPK"
     gene            22497..23246
                     /locus_tag="PK754_02515"
     CDS             22497..23246
                     /locus_tag="PK754_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNB08407.1"
                     /translation="MTPQKDFVYPDLTSIAVILVEPQAPGNIGSVARAMKNFGMKDLR
                     LVRPCDHLDREALWMAVKAEDILHSAQIYSTLTDALKGIQFSVATSNRRRETHFPSFT
                     PREIAQQMAAFSPITSCALVFGGEKNGLSTEDVHACRVLSTVTTAPVQNSLNLSHAVM
                     IYLYEVFAARLTGNPQFVWDMAKPADTEILYERIQRVLEAVKFKPRKTNEGFLLGVRR
                     ILGRTPLEDRDVRLLHKIFQEIERYVKRSEA"
     gene            23289..>23651
                     /locus_tag="PK754_02520"
     CDS             23289..>23651
                     /locus_tag="PK754_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HNB08408.1"
                     /translation="MEFEFISLLFISVCGFAFLAGFVDAIVGGGGLVTVPFLFVGFPS
                     APVSMVIGTNRFSSIPGTIVAARHYLRHTQIPVKTLLLCAFSAAVMSYTGAAVSRLLK
                     PDVLKPLMLIIMVAVAAYT"
BASE COUNT         6485 a         5109 c         5536 g         6521 t
ORIGIN      
        1 tcatcggaac atccgtcatc ttccgtgtcg cccgtatcat tcagcaacac tatcccggca
       61 taatgtaccc ttaattcggc tttgggtttg aataattttt tagaaggcat ggtgcggtga
      121 tcgtgcagaa tatttttttt gaatggaata actacatgat tgcgaatagc ggctgtaatg
      181 tgcggtcgaa aaactaaaaa tagaaaaacc aataaagtcc agcgatgccg tttgaaccat
      241 gattcagata tgattttcat acacaactcc ggtttgataa ccgataacat tcaaaccttg
      301 tgccattctt aaagcttgta tttacaatgc agtcgagctt tagtgagtgt gaaaaaagtg
      361 aatgtgtatc ttatcgcata cgctattttc ttgtagattt ttatcatatt cgctatattc
      421 gaaaagtaat tttgattcaa aaaaatcact tagatcgaat gcaggagcaa acgtatggct
      481 acggcacgta aaaagaaaac cttcaaatac agcgtattta aaaatgaacc gtttacggat
      541 ttttcgaaac ctgcgaaccg taaatcattt gaaaaagcct tggaaaaggt ttctaaagaa
      601 ttaggcaaag aataccatat cattatcggt ggtgaccgta ttcgtaccaa tcacaaaatg
      661 gagtcgcgta atccgtccaa tcgtgagcag ctcattggta ttttttatga cggcaatgaa
      721 gaattagcta ataaagccgt tgaaacggcg gctgaagctt ttaaaaaatg gagccgcgtc
      781 gctcccgaaa aacgggcatc ttatttgttc aaggccgcat caattatgcg taagcgtaaa
      841 cacgaatttt cggcaatgat ggtttatgaa gtcgggaaga gttgggccga agccgatggc
      901 gatacggccg aagctattga ctttatggaa ttttacgggc gcgaagtgct gcgttatgcg
      961 tcggagcaac cattgacgaa aatagccggc gaagataacc aactgatgta tattcctttg
     1021 ggtgtcggcg ccgtgatacc gccgtggaat tttccgctag cgattcttgt cggtatgaca
     1081 acggccgcca ttgtttccgg taatacagtt gtactcaagc cgtcgagtga ttcaccgacg
     1141 attgcagcaa aatttgttgc gttgatggaa gaagtcggag taccgaaagg ggttatcaat
     1201 tttcttccgg gacccggttc cggtatgggt gatgccatca ctaaacatgc attgacacgt
     1261 tttatcgcat tcaccggctc aaagaccgtg ggtttgcata tcaataaagt cgccgcagaa
     1321 acacaacccg gtcaactctg gatcaagcgc gtgatcgcgg agatgggggg caaagattcg
     1381 atcatcgttg acaatgaggc ggatttggat aaagccgtgg acggtgtgtt gtattcggcg
     1441 tttggattct ccggtcaaaa gtgctccgca tgttcccggg cgattgtcgt cgagagcgtt
     1501 cataaaaaat tcactgaaaa attgatcgcc aaagctaaaa cattgaccgt cggcaatccg
     1561 gctgatccta aaaactatat gggcccggtg atcaatgata aagcgctcgg aaaaatgatg
     1621 tggtatattg ataaagccaa acaagaaggc gctgaacttc tgctgggcgg cgtgaaagat
     1681 gacatgcagg gaagcttttt gatgccgaca atttttgata atgtgaaacc caaagatacg
     1741 atttcgcagg aagaaatttt tggcccggtg ctggccatca ttaaagccaa aaattttgat
     1801 gaagcgttag aaattgcgaa caatacggaa tatgggttga ccggcgctat ttttacgaaa
     1861 agtcgtaaaa aattggaacg tgcacgcaat gaatttcatg caggaaatct gtacttgaat
     1921 cgtaaatgta ccggagcatt ggtcggcgtg catccgttcg gcgggttcaa catgagcggt
     1981 accgactcca aagcgggtgg acgggattac ctgatgctat ttacacaagc taaagtcgtc
     2041 agtgaaaaat taaaataatg caacagtttt tgaacggatt cgtaaaaaac cgataggtat
     2101 tatcctgtcg gttttttatt ttactatatg cacgatacag atcccttata caggtcatta
     2161 cagattccac taacgcggct tcgtcgtgaa gagaatcgtc tttattttca aacgaatgcc
     2221 cttcgcggcg cggctttttt gctgttactt gctctcttga cggcaagtat tgaagtactt
     2281 tctgatccgg atgtgttagg gcgatgggta ttggcgatga gcgcacttat gatcgcctgt
     2341 gtgataattg ctttctatat cgtttcgccg attttgcgtt tgttcggtgt tgttgcggct
     2401 tcatcagatt ttgttttagc gcagcagatc ggtcaacgtt ttccggaaat taaagatcgt
     2461 cttttgaatg cgttgcagct ttttggggaa ttgcgcgaag ggaacgaaaa agtttccgag
     2521 cccatgatca tcgcggcgat acaaacaata catactacac ttgaaaagta tgatatcatc
     2581 agcgtagccg atcgtacagt acttcgtaaa acatggcgat atacgtctgt cgttgttatt
     2641 gcggcaagca ttgttttttt gttttttaat cgccctttga ctgacgcatt tgcgcggcta
     2701 gccgatccgg gaaaaaaacc gatggaggtc attccgtacc gatgggtgat agtaccggga
     2761 acgaccgata tactccaagg tcagaatcgt aaaattagcg tcgaaattat tccgctgact
     2821 tccgacgcgg cgatgccgga tgccgtgcaa ctcttaacac agttgtccgg agtagaacaa
     2881 gtgaccgaaa ccctcatgaa aaaagatacg gcacggtttt actctttgga gttacaaaac
     2941 ctgcgtcagg aaacgacata ctggtttaaa ttttcatatg tagaaaatag ccgcaaacgt
     3001 gctgcaattt cagaaaaaaa tacacttcat gtgatgcgtc gtccgtcggt aaaacgcctt
     3061 cagatcgaaa tcgaatcccc gacttatgcg cgcatcgaac accgtatctt agaagccaat
     3121 agtggtgata tacaagccct caaagggtcc cgtgtccggg ttaccatgga agcgacaaaa
     3181 tcactgcaag aagcgcgtat tgtcttttcc gattcgactt caataccgct gaaaacttcg
     3241 gtgtttaatt atgaaaaagc cgaaggttct tttaaagtaa tgaagtcggg aacctatcat
     3301 tttgtgatga cggataaaga aggaacaccc agtgccgatc cggtcgaata tcgcatagat
     3361 gtgcaagacg atttatatcc gttggttcaa ttgaatattc ccgaaaagga cggcgatatc
     3421 accgaagcta tgctacagaa gatcgtagcc gatgtgcagg atgactttgg tgttagccga
     3481 attgtgctaa actataaatt gatgcatacc cgcggtgttc tgcccattcc ggaaacatat
     3541 accacggaag atttaagttt tttgattcgt agcgatcaga gcgaatttga tgtcgcttat
     3601 aattgggaat tctcaaaact gctattacaa ccggaagatg tgatcggctt ttatcttgag
     3661 gtgtgggata atgatgccgt ttccggacca aaaaaaacaa aaagtgtcga gcgtcgtctg
     3721 cggtttcctt cgatggaaga aatttttgca gaaaccaatc aaaaacaaga tcaacagatc
     3781 caagcggctg aggaaattgc caaagaaagt gaagaaataa aaaaatcgct ggaagaagta
     3841 catcgtgaga tgctcaaaga taaaaaaccg gagtggaagg acaaggaaaa gataaaacag
     3901 cttgtcgaaa aacaagagga gcttcaaaag aaagcggagt ccattcaaaa aagcttagaa
     3961 gaaatgacgg ataaacttaa agaacaaaat ctgcttacac ccgaaacggt tcaaaaatat
     4021 caggaattgc aaaaactact tagcgagatt aattccaaag aaatgcagga attgatgcaa
     4081 aaaatgcaga aggccatgga aaataatttt gaccctaacc aaatgcggga tgcacttaag
     4141 aattttaaaa tggatcaaga ggcgttcaaa aaaaatgtgg atcggactgt cgaattgctc
     4201 aaacgtatca aagccgaaca gatgtttgat cagttacgcc gcacagcgga ggacttggaa
     4261 aagcaacaaa aagacctgga tcggatgacg tcgaacttaa aaaacgataa tgaacgtacc
     4321 gccgcaatca aagaacaaga acgcatccgg aaaaacaccg atgatctgaa aaaaaaatcg
     4381 gatgagttac aaaaattggt aaaagaatta aataataatc aatccaatca aaaactcaat
     4441 acggcgcaag atcagttgca acaaaacaac attcagaacc gcatgcaaca gtcacagcag
     4501 gccatgagcg ataaacaaga aggaacgaaa gaagaaaaag aaaacagaga ggatattcat
     4561 aaatctttag attcgctgtc ccgatctatg gctgatgcac aaaaggaaca tcgcgccaat
     4621 caggatgaag ctataaaacg tgccatgcaa aagatcgtcg tagatatgct ggtcatttca
     4681 caaaatcagg aggctttgat ttccgagact tcgccgttaa attttgccag cgttcgtttt
     4741 cgtgaagtga cacaagatca gtcagacatt ttatccaata tgggtatcgt tacggaaaat
     4801 ctcttggagc tttccaataa aacctttttt gttaatacgc cgctgggcaa aaccgtcggc
     4861 caggccatgg gacaaatgca aaccgccgtg aaggccatgg aagaacgtaa tacgccacaa
     4921 gctgtcaatt cacaaacagc ggctatggta tccgtcaatg aagcaatcaa gttattgctt
     4981 ctgagtatgg ataagatgaa taacggccaa tcgggtacag gaatggaaga actgatggaa
     5041 cagatgcgtg aggcggccgg aaaacaaagg aggattaatc agggaatgcc atcgttgatg
     5101 gagggccaag ataataaccc gggcggacta tcgcaagaac agcaagctca actcggtcgc
     5161 atgatggctg agcaacaagc actcaaacaa tctatggaag ccatgaaaga tcaagccggc
     5221 gctcagcaag gcttaaaagg gcagttgggt aatttggcta aagagatgga agaggttatc
     5281 aaagacatgc agtccaatca agttaatcgt aagaccatcg aacggcagca gaaaatactt
     5341 cagcgtatgt tggatgcttc gacttccatg caagaaaaag atatgagcga aaagcgaaaa
     5401 ggcgaaaccg gaaaaaatta tgatgcggtt tctccgcgcg cattgcctga aaatctgacg
     5461 gatcgtcgca attttttacg ccaggaatta ttgcgccaaa tgaaacaagg ttatgctaaa
     5521 gattatcagg agctgatcaa acgttatttc gacgaacttg gcgaaaaaac cggtttacca
     5581 aaaaacgatt agcgcatcat cggaatatgt acgttctttt ctttggctac tttgatggct
     5641 tcatcgtaac ccgcgtccac atgtcggaca acgcccatac cgggatcaca cgttaacacc
     5701 cgttttaacc gcacagccgc ttccggcgaa ccatcggcga cgatcaccat acctgcatgt
     5761 aaactgtatc cgatacccac accgccgccg tgatgcagcg atacccacgt cgcgccattg
     5821 accgcgttga tcgcaaaatt taacaacggc cagtcggcga tagcatcaga gccgtctttc
     5881 atggcttcgg tttctctgtt aggtgaagcg acagaaccgc aatctaagtg atcacgaccg
     5941 atgacgatgg gtgcttttac ctttttggta cggaccaatt cgttgaaaat caaaccggct
     6001 ttttcacgtt ctccgtaacc aagccaacaa atgcgactgg ggaggccttg aaaagctata
     6061 cgatctttgg cttgcgacaa ccaacgttga agacctgcct tttcaggaaa agcctccata
     6121 atagctttgt ccgttacata aatatcttcc gggtcaccgg agagcgcagc ccaacgaaag
     6181 ggaccttgcc cttcgcagaa taacggacga ataaatgccg gaacaaagcc cggaaaatca
     6241 aacgcattgg tcacgccgac tttaagcgct tgcccgcgaa tattattgcc gtagtcaaat
     6301 acgatcgctc cacgactttg aaaatcaagc atagcgcgca catgaatggc catcgattcc
     6361 atggcccggc caatatattc ttgggtgcga tttttacgcc attcggtggc ttcttcgacg
     6421 gagatacccg taggaatata gccgtttaat tcgtcatgtg ccgacgtttg atcggttaca
     6481 atgtccggga tcatattccg tttgagcatt tctggtaaaa gttctgcggc atttcccaat
     6541 aagcctatgg atttggcttc gcccttggct ttacactccg tagccatacg ccaggcttca
     6601 tccagcgact cgaccatgac atcacaataa cgcgtatcca aacgacgttg gatacgggtg
     6661 cgatcaactt caatacaaat agccacaccg tcattcattg tgacagccaa tggctgtgca
     6721 ccacccatac cgcccaatcc ggccgtcagc gtgatcgtac cgcgcaaagt gcctttaaaa
     6781 tgtttgcgcc cgcactcggc aaaagtttca tacgtacctt gcaaaatgcc ttgagtacca
     6841 atataaatcc agcttccggc agtcatctgt ccatacatga tcaagcctaa tttttcatat
     6901 tcgcgaaaat tttcccatgt tgcccaatgc ggaacgatat tggaattggc tatgataacg
     6961 cgcggcgcgt cggcatgggt tttaaaaacg gcgaccggtt ttccggattg aacgagcaga
     7021 gtttcatcca ttgccaaatt agtaaggctt tttacgatcg cgtcaaaaca atcccaattg
     7081 cgggctgctt tgccgatgcc accatacacc acaagatctt ccggacgttc cgctacgtcg
     7141 ggatcaagat tattcatgag catgcgtaaa ggcgcttcgg taagccagtt tttacaggta
     7201 attgaagtgc cgcggggtgc gcgtatggtt cgcatagatg aattccttta tgattacaat
     7261 tattttggtt ttgtagatcg aacaaaacgt gcgatgatgg ccgccacgga ttcgacggca
     7321 tgaacaccgg tgacgctttt accggcttga taataatcgc gagtggacat cttgccttgc
     7381 aatgatgagc gctttaactg gcgcatggaa cgcaacgcgt aaatcaaacg catcattttt
     7441 tttaattggc gatgtttgag catcaggcgc gctatggtgc ccgttttgta cccgacgcgc
     7501 tcgacatatt cggtttttat cacagccaat ggtacgcctg taacgcgttc cgtatgaacg
     7561 atatctgctt cgccggcttg tacgatggct cgtttgtaat cctcgtgtgc cgtacattcg
     7621 gtcgtagcaa taaatcgcgt agccatcatg acgcctgcat aaccgatttt caaggcgtct
     7681 gcataatcgt tttcatcacc gataccaccg gcgcacacaa tcggtaaacc ggtatcggcc
     7741 agcgcatcat atagttgttg cggcgagaga tggccggcat gtccccccgc gcgggcattg
     7801 acggctatga gaccgtgcac tccggcatcc atcgcttttt ccgcccattt gcgttcggtg
     7861 actttatgat agacgatgcc ctggtatggt gcgacacgat ctacgaccca gcgcggattg
     7921 ccaagcgatg tgtcaaaaaa acggatacct tcttccagcg cgatatcaat ccactgccgc
     7981 aaacgatctt cataaacgcg cgacgacttt tcgactgtcg cgttgagtgc tataggctta
     8041 tccgtgagag aacgaatata acgaaggcct tggcggaagt catatttatg aacgtaggta
     8101 agtgataagg gttggatgat gccgagtcct ccagccgcag aaacagcggc aacaagctcc
     8161 ggattgctgc acggatacat agccccgcaa agaatagggt attgcacgcc ggcatgacgc
     8221 gtaaatgcag tttcattgac catgatttgc aagctctctt agtatgagac cgtaacgtaa
     8281 gccaaaattg ctgaccgtga ttttatctaa attaaaaaaa gaaaatattt ccgataaaat
     8341 cgttgctccc gcaataataa catccgcacg ctccggttcc aatcccggta tcttacgacg
     8401 ttcttcgagg gtaaggtcgc tcagtttttc gatcagcgaa tccaacgccg ctttatcaat
     8461 catataacca ttgatgcgct taggatcata atgcgggagc gcttgcaaca tcgcacataa
     8521 tgtagtaacg gtaccagcaa caccgagtgc atgggccggt tgaatatttt ttataccgga
     8581 taaatgagtg tcaaattcgt tgtgagcata ccggcgaaga ctttccaatt cagattggag
     8641 aggcggatca tgattgataa aacgctccgt catacgaacc gcgccaatgt tcaaactttt
     8701 tttgaacgtc atatcggaag cggagccgac aacaaactca gtactcccgc cgcctatatc
     8761 aatcaagaga atggggtggc gcgtcaatgg gggaagagca aggcaaccgc cttgaaacgt
     8821 ccactgggct tcttcatcac cggttaaaat ttgaatacgt tgaccggtac gatgataaat
     8881 ttcatttaaa attgtttctt tgtttgcggc gtcgcgcatg gcgctggtgg cgccgatgac
     8941 gatctttgcg gtctgatgat ttgcggcgtc ttgcaaatat tcggttaaaa catgtgtgag
     9001 tttatccatt ccctcgtggg cgatattttt ttgtgcgtcc acgttttttc cgagtctgat
     9061 gatacgttgc ttttcaaaga gcgtgtgcaa atgaccgctg gaatccgctt ctgcgatgag
     9121 taaaagtacc gtattggtgc cgatgtctat tgaggcgaac cgcatcacga tgtcctttgc
     9181 agtacgtaag cgacccattc accgtcggct cgaaattcaa tgacatgcag attgtgttca
     9241 gaaaacaaca tgcgcatacg ttcgtcttcg atggcgagaa ttccagaaag aatcatcatt
     9301 ccggtgggtt tcattttttg attcaaattt tccataaacc cgggcatctc gaataccgcg
     9361 cgttggatat tggctagtat tatatcgtaa cgttcgggcc atttagaggg cagggtttcg
     9421 atagtggtta gtaccgtttt tacacgggct gaaacgcgat tacgacgaat gttttcttcg
     9481 gcattttcaa aggcatttgt atccacatca aagccataca cagcgcgtgc tccggacttg
     9541 gcggcggcga tcgccagtat gcccgtccca ctgccgacat ctagcagtat tttattctta
     9601 acggaaaacg attcgataaa acctaaaact aactgagttg tttcatgtgt tcccgtacca
     9661 aatgacattt taggatcaat aaccaaaatg atgtctttga gcttacgttt gtatttttcc
     9721 catgaggggc gcacgataaa gtgttttcca acacgtcgcg gtttgaaatt tttcttccat
     9781 tcgctttccc aatcttgagc ggccattggg cgaatgtgaa ccgtcgtgcc cggaccaagg
     9841 cgcggtgcga gtttggcgcg aagtattttt tctgaggaat aatcggctgg ttgaaaatag
     9901 gccgtgagcc ccgttgccgt ttcgatggag ccggtcgaac ccaattcgaa taataaagtt
     9961 tgtgtagctt cgagattaac gatatgaccg agatcaactt cccaccagaa gtcttttttt
    10021 ttcttagtac gtgtgctgga acgtgatttt tttttcttgg gttgcatgat tcacggcgga
    10081 accgcccgat attatttcag cgcctgaaaa acggcatggg caagcgattc attagtttgt
    10141 gccttgtcaa caaatgcctt gatatggtat ttgcgaagcg cgtcgtgttt ttctaactga
    10201 tcctgatcgc cgtacacgat gagcggaatg ggttggcggg atggcctggt gagggctact
    10261 ttgttaagga ctaattcaca atcttgcgga tctacgatca ccacatgcgg atcgtatttg
    10321 gaaataatgt cttgtgtcac ttcatccggg ctcgttacgg tcggagcata gccggccgat
    10381 ttaagagcgc ggctcgcaag aattcgggga atacggtcgc ggtcaatcac gagaacacgt
    10441 ttttgatctt catcgcgtat ttctttggct tccaattcat caacgatctt ggaggcttct
    10501 aagatgaggc tcgttacgct agccgaaaaa ctggtatttt gagagcgtac accttcctga
    10561 aaaatataag tgatatcttt catcgatgcg agttgaagaa aagcgtcttc tttgatggca
    10621 tttttgcttg tcgcgtaaac gatcttgcct tcgctgacaa atacgtcgcc gactttattt
    10681 ttggatttat caaaaaattc gaaacagccc gtgcgacggc tttgttcttt ttcctgtgct
    10741 tcggcaaaga aatggtgaat gggtgtataa cggcgtttga gtttggactc ggcgatgcgc
    10801 acttttccgc gtgtgataac ctctgcgatg tgaaaaggtt tgcttatggc atcaacggcg
    10861 ccggcttcca gtcctttgac gatttcctcc gcattaaaat ggccggtaac gaaaatgacc
    10921 ggtatatacg ctgtttcctc gtcttcttga agttttttca aaagttggta accgctcatg
    10981 ccgggcatca tgacatccat caggatgagc tgatacgatg tcgtttttac atagcccaat
    11041 gcttcttttg cattggctgc gtaatcaact tcataaccgt catgctggag tacgcgaata
    11101 agcgggtcaa cggcgtccag ttcgtcgtca accacgagga tgtgatcttt catggcagtc
    11161 tatggtgtat aagtaaaata tatttgaaaa ctattcttct ttcccaagta accgtctgca
    11221 tttactgatg attttattgg tcggcgatgc gatgacgctt tggcgcggga tttcttcctg
    11281 tgtaaatcga aaaaatccgt cttcccagta tatcatgtca taaatcgcgt cttcgccttg
    11341 aacgtgaccg agcgtagcat taaaaatttg acctttcata aaatacacgt tgccttcgat
    11401 cgtaccgtct gaaatcagca ggttgcctgt tttagcggta ttggctaaaa gctggagaac
    11461 gtcgatcaca ctgacgtcgc cgagtttacc gagaaatccg tcacgcaacg tcgctttttc
    11521 tttttcaaaa gcattaacct gactgaccat gctttcgatc cgaagcaaaa gatcttgtcg
    11581 tttggtatgg cgtttgacga gataatcatt ggcaccgcag cggaagccgc ggatttcggt
    11641 cagcatgtcg tcaatggccg taaaaaacac gaatggtaca gttggcacgt tggatgtatc
    11701 gcgaatccgt tggcacaact caaaaccatt catctcacca agaaaaatat cgctgattac
    11761 cagatccggt ttttcgcgat tgacgatttc caaggccacg tttccgttgc cggtatcaaa
    11821 aacgatataa tcttctttga taagattttc tttaataagc aagcgcgcct gttcatcggc
    11881 atccacgagg agcagtttgg agcgagtttt ttttgtttct gccatagccg tttttctcct
    11941 cagcttcagt tcagtttgtt ttcatcggtg atgcggcaat attccatcaa aatattcatg
    12001 gtggccgaat ggatggtcgg tgtcaccttt ggttcaacgg attcaaattt aaagacccca
    12061 gttttccatt cgccgagatt atacacagct tcttcaccga caaactgttt gtattcggca
    12121 tgcaacatgc gtccgtcttc gaatgtgatt tcggctttgt cgctgtcacg ggtgatcgtc
    12181 agcaagcccg tttttttatt cataatcaaa agttggaata catccacaag acttaagtcg
    12241 ctaaatttgc catacaagcc ttcttgcatc tgattttcgg cttgtttgta ttcgtttgat
    12301 ttttggagca tggcggatac ttttccgacc aactcttctt ttttaatatt cgatttgatg
    12361 agatagtcgt ccgctccggt acgaaggccg cgtaattccg ttgcaacctg atcaatagac
    12421 gtcagaaaca taaagggcac catgggtgat tttgaagttt ctcgtacttt gcggcaaaat
    12481 tcaaaaccgt ccatttgcgg catgataatg tctgatatca cgagatcggg taaatgatca
    12541 ttgaccatct gtaaaccttc ggcgccattt tcggcttcga tgacttcaaa attttctttg
    12601 cgcaaattaa tgcttaataa tgtgcgcacg gttttgttat cgtcaacgat cagaattttt
    12661 ttaggcatcg gaaaaagtcc ttactctctt tatttcataa attaagcata aattccacgg
    12721 agttttaaaa cctgagtgac gcggtcaagc gccagtatat atgcgccgat gcgcatggat
    12781 tgggtgtggc gaagtgcgac ggcatgcacc gattcgaaac tttcgatcat cacacgttca
    12841 aggcgatcat gtacttcatg caatggccaa aaaaaaccca ttcggttttg cacccattca
    12901 aaataggaaa ctgtaacacc accggcattg gctaaaatgt ccggtacgac caagatgccg
    12961 cgttcatgca gcaaagtatc agcctccggc gtgatcgggc cattggcacc ttcgcatatt
    13021 attttagcct gtattttgga tgcgttctcc gcatgaattt ggttttccat agccgcaggt
    13081 attaacacat cgcaatccaa catcaataat tcttcattcg ttattttctg tgcattgggg
    13141 tatccggata aagaacgacg gttttgttga gcaaatcgga tcgcatctga gatattgata
    13201 cctttttcag aaaagtaaga cccgctcgca tctccgatcc cgatgatttt gcatccgttt
    13261 tcatacaaaa gttgagcggc ggtcgcacca acggaaccaa acccttggat tacgatgcga
    13321 gcttcgttcg ccggtatgtt acgctgtttc atcgcggcaa gagcaatcag cataataccg
    13381 cggccggtgg cgtcgcgtct tccgtgagaa ccgcccatga tgagcggttt tcccgtcacc
    13441 acggcggtct cggttcgccg gacatgcatg ctgtatgtat ccatgatcca tcccatttcg
    13501 cgttcgccgg tattgttgtc cggtgccggt acatctttat caggaccgaa aatttcaaac
    13561 aaattggccg tataacggcg gataattttt tcgtacgccg attccgtgag tcgagaagga
    13621 tcgcactcaa tagcgccttt tgctccgccg aatgggatat ttactacggc gcacttccac
    13681 gtcatccatg cggccaaagc ggttgtttca tcaagcgtgg ctttcggaga aactcgaatc
    13741 cctcctttgg aggggccccg aacggtgcta tgtatgaccc ggaagccttt aaaaacttgg
    13801 attgagccgt cttcaagtac tactggaatg gatacagtaa cttgtttttc aggcgtaagg
    13861 aggtattggc gaacacctgt atccaattcc aaaatatccg ctgccttttc aaaaccacgc
    13921 atcattgggg atgaattcta acatttaaaa agtaaaacaa tacgtccgat ggtgattact
    13981 atcagcatgc atagcaattg cagatcgtaa ttgaaaatac agtcgcttaa gttgaaaata
    14041 atattaaaga tagagcaaaa aatcaaatag atttatatct agtttcaggc caatacgtca
    14101 tcttagaata gatgaagatg gagtgccata ttaagggtat aaccggataa gtcaattgtg
    14161 cgtgaaggaa tgtgtttttc gtgcgtttcc ataataaatt cacgaggacc aatttgtttg
    14221 cgaataacat attcaaaaga ttccgaagga agttttattt tctcggtgat tctcttttcg
    14281 tagcgaaatt gacagctgat tttaggagac aaataaaaat ccaatgacaa atcagctacg
    14341 gctcccagcg catttcggtc gtatgaaaat gaagatgaag tttgatttat gagttgtgga
    14401 tggctgtgat atccaatatt ttgtgcgtaa tagtgatgcg atgaggtatg atatgaataa
    14461 tatgcgatac ctgcaccagt actcaattcc agtcgtgtag tcatattaac aggcataaat
    14521 atatatctac cgcctacctg taacatttta atttctgatt tttgttctaa gtcaaatctt
    14581 tgggaaggcg gagtatcttc ttcgttaaca aattgtaacc aattgcttcc tgctaatgtt
    14641 tgattaaaaa ttcttgacac aacagcctct atttgccaat gtggtgaaag gttatgttga
    14701 acagatattt gaattaattt tgggttatgt tttttttcat caggatcttt caaatcagcc
    14761 acatgatata cataaccttc attatcataa aactgttcga tattgtttcg ttcatgaaga
    14821 taagaagcgc caaaaatgaa attgatatgc caataggatg gcttataatc agccatattt
    14881 gattttattt tatttcgttt tgcgtaatcg gctgccgtaa tagagtctaa agtgttagcc
    14941 gaatgtttgg taacatcagg aacataaggt cgtctattga tttcagggtc atatctatcc
    15001 tgcgtatata atggaaaacc agaacaatat aaaccgattg ccatccaaaa catcaatatc
    15061 gtacgtaatg gatttgttgc acacactata caacacatat caatttctcc ccgaaattac
    15121 tcagactact taaaatgttt ttatttgttt tttgcaggaa acatagtttg ccatacgtaa
    15181 tcccaaatgg gtgtactgac tccgaaccct ttttccgggt tctgaaaatg atgtttcatg
    15241 tgatgatttt ttatagctaa ccaaaatgcc gaatgcatgt tgaagtgatg aatggcataa
    15301 tgcgtcatat cataccacaa atacccgatc agaaaaccgg caaacatggg agcgatattt
    15361 gatccaaata tgattaaaaa taaaaaatag aataacgcag ctaacgggat gctcgctgcc
    15421 gggggcatga caaggcgttt agaatcattg ggataatcgt ggtgtacacc gtgaaaaatg
    15481 aaatgtatgc gttccatcag agcgctccgc ggtgtgaaat gaaaaataaa acggtgcaaa
    15541 acatattccg tcagggtcca cactgcaagt ccggctaaaa tccaaaacaa aaagtgtttt
    15601 atatctgatt tttcaatcca taaactttga tagatgaagt aagaaattat aggaataaat
    15661 acgatcaacg gaacggagaa gtgtactttg gaaaagaact ccaaaaaatc actttcaaac
    15721 attcgcggcg tgacatcttt gttggtaatg ggtttcttgc tcatgtaagt atttcttaaa
    15781 tattatatga aaataactca catcgcgatg tgcatctttt ttgggggatg ttatacgatc
    15841 aacgatatga gatttaatga tgcctttctt aaggtaatag tagtgatttt gtatagaaaa
    15901 tcaagttgtt ttttgaactg aaaaatgtac tttaccttag cttaaagata aaagtattat
    15961 attgaactga atttttgata taggatcaaa ttatatgttt gagacgcaca tcatcgaata
    16021 cagcgccaag tccgaaatgt atgcggcatt gcatgcccag attcatcatt tgcttgatgg
    16081 tgaaaccgat ctcattgcca acgcagctaa cgcctcatcc ttattatttc atacgttgcc
    16141 ggcgctgaat tgggcgggtt tttattttta caaaggcaat gaattgatag tgggtccttt
    16201 tcaaggtaag cctgcttgtg ttcacatcgc tatgggcaag ggagtatgtg gtacggccgc
    16261 tcaacagaga aaaactgttg tagtgaagga tgtgcatact ttccctgggc atatcgcgtg
    16321 cgatagtgcg tcgaattctg aaattgtggt gccgctgatt tcgggaggac agttattcgg
    16381 agttttggat ttggatagtc ctcagatggc tcgttttgac gaagcggatc aggccggttt
    16441 ggaggaattc gttgatattt ttatgaaaca taccacttgg ccaaagaatt tatagcttag
    16501 tagcaagatt gattttcttt tttaggatac gtgctcttgc gcgaagcgcc gcactgcctt
    16561 tcatttccat ttcatccaat aatccccaaa ctgtaggtac taaccacgta tgccgtaatg
    16621 cgatatcagc taagccggtc atcgtattga cggtgacaaa tttattttta gtggtgtgtg
    16681 cgagttcaag caaacgcgta ccgattcgtg tagcatcgtt ttttttccac gcgacgtaac
    16741 ccaaaatcag aggcagatgc atctgtaggg cttcattgcc aatcaacttt ggccagtctt
    16801 tgttcagata tggtgatagt gtacgtatgg cggcggtttc gtttcgtagg attttttcta
    16861 acgtatcaga agcgcgaaaa caaatatccg catcttcgtg cgataggaga tggtataaat
    16921 ttttaagccc cgttctatca tgagtaagct tactcactag caaattcgca tgagcgatgg
    16981 ttcggcgatc gttgccggta agcattttta ttatttcact tttagtcatg gctgtcgcca
    17041 agcgttatgt ccaagtcagc tatgggatga ccattgatga tcaaagtaat tgcatgttta
    17101 ccggaataat gcttgcgcgt cgtaaaattt tgaaaacgat gttttttact caacttcaac
    17161 gaaccgctat cgtaaagacc ttcacgccat ttgaagactt tgcggctgat ttttcctccg
    17221 gatttgacaa aatcaatcgc gtattcgatg cgaagtgttt tggctttccg tatccgaatt
    17281 tccgtttcaa aatccaatgt atcaccgata aatatttttt tcttcttgga tacgaatgaa
    17341 agtactgaaa cgccggaggc atcgtgcgta ccaaaaatag tgagcgcttt tttattgccc
    17401 cgttttaaaa gtgtgcggca ggcgtgtttg accaatttgt ctgtttccgg agacaaacct
    17461 cgccagcgtt ctgcgatctg cagaacgatt tccggattat ctttggttat gtcgttcagg
    17521 ttattcgcca cgcttcggcg aacatattct gaaggatcag ccttaagatt ttctaaaatc
    17581 ggcaaaatcg gagccgggtc cttttgaaac acaggcaatg cgatagccca cggcagtcgc
    17641 ggacggcaac cctcgctggc caatcggcgt acatgcgggc tactgtgccg actccaaacg
    17701 agcatttgtt gtaacatgat gtccggataa cgaagaataa aaggtctgac ggcgaattct
    17761 gcacttgaaa cttgggtgaa taattccaat gccggtatgg atgactccaa atcatcaagt
    17821 ccgtagtgtt ctacgaaatc cggaaacacc atatccgtaa acgatatttt ggtgtcattc
    17881 atacgaaaat gtttcgttac atgaatgaga atgccgagcg cctttcggta atcctccggc
    17941 aaatgagatc tgagagcgga ggaaatatga tgcatccgat ctttgagttc tcgctgagac
    18001 catgcgtgat caaatataaa attcgtaaat gcccgtttgt caaaactcga ataaacggtt
    18061 tgaatctgat cgcctaaagt ttcgacaaac gatccggaaa acatattttt taatggttct
    18121 gccataggtt atgccgttat tttaaattcc ggattgtaca tcaatccgat cacattacca
    18181 aacggatcac gcactgatgc gatgataaga tcgccgccga cacagtttgg atgctcgttg
    18241 gcttcagctc cggaccggat aagatgatca aacatagcct gcacattgtc ggtgcgccaa
    18301 taggtaatac tgccgaaccc gggtgaattg tattgtttag gatccggatc gaggccaagt
    18361 tcgaatccgc cgaattgaaa tccgacataa aaaggttgat cgaaataggg ctttacgcca
    18421 aacgcatcag tataccatcg tttggctgct tccagatcgg tgacgtgttg tactacagag
    18481 cacaacccca atattttggt attcatcgtt atgctccttt ctttaagaaa ttatcaagac
    18541 gatttttttg atacaagtga tgccgaaaat gcatttggat catggcaaac cattcgtcgc
    18601 cgctcaaata tccgaatcgg gggtgttgtg ccttgccctg atttcgtttc tgtgatagtt
    18661 cgctagctac cgttcgcatg gttccgatca acgtgttcaa atcgcggcgc atggcggcga
    18721 tggattccgg ctgtttgggg gtgtacatgt cggaaggggg gactttgatg cgtatcgggg
    18781 gaaaacggcc cgtaaaaaaa acaatttttc cgggaaaagt ttttttctca tgagcatggt
    18841 ccgggttatc catacactgc cgtaccatgc ggagatgaaa attttgtgtg ccgtgtacga
    18901 ggtggtaata cacctgccca atggaccaac tttcttgatc cggtgcgcga ttgaattttc
    18961 gctcatcata cgtatccaaa gccatcatcc aatcgtttgc gatggattcg aattgatgta
    19021 agtgtgcagt gatattcata ggtaatttca aatgaatgtt taatgtgaaa aacgtgaatt
    19081 aatagtggcg aacatgaccg aaatagacat ctttttcgag agcattcatt aaacgagagc
    19141 acgattcggt ggaatgaaat ttttcaaagg cctgatcggc atgatcctta gtcttccatc
    19201 gtaaaatatc tacaaaaaca cgcgacgtgt cgtcggcccg catattttcc catgatataa
    19261 atccttcgta cgtgccgata atggatttgg cttcggcgac aatatccggc atacgcgaca
    19321 caaactccgg tttgatttta ataacagcca cttccgtgat ataatcgttt cctggtgatg
    19381 atatgttagc ttgacaggca tttaaagtca aaattaacat gatgtttaaa aatactgaaa
    19441 tacaactttg gagattcata ggtgcctttc aatttagttt ttgttaacta tcgcacaaaa
    19501 aataatgtgc atgactgaca tccttatgtc agcaggtttt taatttttga taattacccg
    19561 gcttgacaat agatgggacg ttttctacat tgcgcgggta ataaaccgaa tccattatta
    19621 aatgacattc tcacaatcca tgcactccga caaaatgcgg gtggctttga cctccgtatt
    19681 ggcggcaatt tttttaaccg gtatgaaatt ggccgcaggt ttagctacag gcagtctcgg
    19741 catattatcc gaagcggccc attcagcgct ggatttgggc gctgcgcttc tgacgtatgt
    19801 agctgtacgt atatccgata aaccggccga tgacgatcat tcctatggtc acggtaaagt
    19861 cgaaaacatt tcggcgctgt tgcaaacggg attattgatc ctgacttgct tttttatcat
    19921 tcgcgcggcc gtagagcgtt tgatgtccgg cgatgtgcag gtggaggtga atgtatggag
    19981 ttttgttgta atcatagttt ccatcatcgt ggattattcc cgttctcggg cgcttgcgcg
    20041 tattgctaaa acctatcgca gtcaggcgct ggaagccgac gcattgcact tttcgtcaga
    20101 tatatggagt tcgtcggttg tcatcatagg ccttatttcc acagcgttgg gatttcatca
    20161 aggcgattcg cttgcggcta tcggcgtggc gttatttgtg cttgtgatca gcggcaaatt
    20221 actcaaagaa acgattgatg ctttgacgga ttccatgcct aaagatattg aacagcgcat
    20281 cagaacggcg ctggtcagtt tagacggtgt ggagcggtat cgccatttgc gggttcgtca
    20341 gtcgggttcg aaagtgttcg tagacatgta tattgatgtc aagcgtacgg tgcctttcga
    20401 aatggcgcat catgtcgccg atgctgtgga aacgcgcatt catgaaattg tgcccaatgc
    20461 ggatgtgatc attcatatgg aaccgcagga aaccgacgac gaaagtatca ttgataaaat
    20521 ccggatgatc gtcgtacaag aaggtcttgc ttgccataac gtgcgtgcac aacgggttac
    20581 caacggatat tttgtggatt ttcatttgga atgtgaaagc caccagcgat ttgacgaagc
    20641 gcatgaagcg tcgtcgcgca ttgaaagccg attgatggaa aaaatcccgg agattaagga
    20701 tgtaaaagtg catatcgaag atgcgcggga tcgggttgtg caagcatttg acattacgga
    20761 ccaaagcaag ccgctcatcg ccgccgtacg ggctattgca gctaatacgc agccggtgcg
    20821 tgcatgcagc gattttatcg tgatggatat cggaggtcat aaaaaattga tgctcaactg
    20881 cgtggtgccc ggcgatttat cgctcgatga ggtacacgcc ataacgacaa ggctcgaaaa
    20941 ccgaattcat cgggatatac cggaggtttc atccattgtc attcatccgg agttagcgcg
    21001 tgcctgaacc ctccatgatt ttcgaagaga aagacggatt gcgtctgtgc cggtttccgg
    21061 cgctcgaaaa atttcccgac gtgatggccg tgatgtccac gcgcatcggc ggtgtcagcg
    21121 agggggatta caaaggactc aatctgggac tgagcacgga tgataatccg caccatgtcg
    21181 aagaaaatcg gcaacggttt tggcgagcgc tcggtgtaga tatgaatgat gtaattcggc
    21241 agcatcaggt gcacgaagat cgcgtcgttt ttttgcagga acaaaccctc tgtgccgata
    21301 ccgatgcgat gtataccgat cagtcgaaaa aatttctgac cgtcgtcgcg gcagactgtg
    21361 taccggtttt gttttatgag cccgcgcatc gtatcatcgc tgtgatacat gcaggctggc
    21421 gcggaacgct gaaacaaatt atcaaaaaaa cgatggcttc tattcaacaa acattcggat
    21481 gttcattggg tcatgccgtg gcggtgcttg ggccgtccat cggcgcggat cactacgaag
    21541 tcagcgatga ggtggcgtca atgtttgata cggcgtttgt caatcgtcgc gactacccga
    21601 aaccgcatct tgatttatgg aaagccaatg ccgcacaatt agatgaagcc ggcgtcggtc
    21661 atatcgtcgt gaccggtgcg tgtacgatgt gccaaccgga tatatactat tcgcaccgcg
    21721 cttccggggg ccgttccggc cgtatggtcg gcgtgattgg gaggaggaac tgacccgatg
    21781 gatcatatcg aaaaatattt cggatcattg atacagcgtg ccggcgatgc gatttttacc
    21841 gtcagcccat cgcagattat tttatcgtgg aacgatggag cgcgtgaatt gctcggatat
    21901 gacaaggaag acgttcttgg ccacagtgtg gatgttttat cgccgctgga tgacaagcgt
    21961 catatgcgtg caatggcaat tgaggtgttt gatcacggtg cgctcaaaaa tttggaaacc
    22021 gaacttaccc gcaaagacgg acacgtcgta tcggcctacg ttaccgtttc accggtgacg
    22081 gatgaaaacg aaaatgtcgt agcggtatcg ttcatcgcca aagatataac ggatcagaac
    22141 cgactactga ttcacctcat cgaaagccaa cgtcaggccg ggcagataca agctttagtc
    22201 gaagcattga ccacgctgtc gcaccatatt cgcaatgctt ccgcagtaat atccgccaaa
    22261 agtgaagtgt cgctggaact caatcaactg gccgtttatc acgatctggt ccgggtatgt
    22321 acgcatgaaa cgcgtcgtat aacggcggtg attgatactt tgcaggactt ggtgcgcgag
    22381 atgcaagtca gcggcgaacc gattgatacg gtgcaaatga acggtgcgcc ggcccctctt
    22441 ttagatatcg aagcccgcct caaggagcgg ctcaaaaagc ttgatgaaag gccgaagtga
    22501 cgccgcaaaa ggattttgtt tatcccgatc ttacctcgat agctgttatt ctcgtcgaac
    22561 cgcaagcacc cggaaatatc ggttcggtcg ctcgcgccat gaaaaatttt ggcatgaagg
    22621 atttgcgttt ggtgcgtcca tgcgatcatt tggatcgtga agccttgtgg atggctgtca
    22681 aagcggagga catacttcat tcggcgcaga tctattcgac acttacggat gctttgaaag
    22741 gtattcagtt ttctgtggcc acaagtaatc gccgtcgtga aacgcatttc ccgagtttta
    22801 cgccgcgcga aatcgcgcaa caaatggcgg ccttttctcc gataacttca tgtgccttgg
    22861 ttttcggtgg agaaaaaaat ggtttgtcaa ccgaagatgt gcatgcgtgc cgagtgctct
    22921 ccaccgttac gaccgctcca gttcaaaact cgctcaatct ttcgcatgcc gttatgatct
    22981 atttgtatga agtgtttgct gcgcgactta cgggcaatcc gcaatttgtg tgggatatgg
    23041 ccaaaccggc agatacggaa atattatatg aaagaataca acgcgtactg gaagccgtaa
    23101 aatttaaacc acgaaaaacc aatgaaggtt ttttactggg tgtccggcgc atattggggc
    23161 gtacaccgtt ggaagaccgt gacgtgcgtt tgctacacaa aatatttcag gagatcgaac
    23221 gctacgtcaa gcggtcggaa gcgtaggctt cttttatcat attacattat caaacttaga
    23281 gattattcat ggaatttgaa ttcatttcat tactatttat aagtgtttgc gggtttgctt
    23341 ttttggccgg atttgtggat gcgatcgtcg gcggcggggg actggttaca gtaccgtttt
    23401 tatttgtagg atttccgtcg gctccggttt cgatggtgat cggtaccaac cgcttttctt
    23461 cgattcccgg aaccatcgta gcggcgcgac attatctgcg tcacacccaa attcctgtta
    23521 aaacgctctt attatgtgcg ttttcggccg cggtgatgtc ctacacaggc gccgcggttt
    23581 cgcgattact caagcccgac gtgctcaagc cactgatgct cataatcatg gtagccgtag
    23641 cagcctatac a
//
LOCUS       DAOQNQ010000019        23410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_746_length_23410_cov_6.370328, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000019 DAOQNQ010000000
VERSION     DAOQNQ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 23410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23410
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_746_length_23410_cov_6.370328"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PK754_02525"
     CDS             <1..518
                     /locus_tag="PK754_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNB08409.1"
                     /translation="KPKYYHKMIGGNFRLDAIQAAVLNVKLPHLDNWSQRRRENADLY
                     TQLFIAAGVAEAEGKITYDSKNKVLLPKAAYRTSGAKNHHIYNQYVIRVEKRDALRKY
                     LTDKQIGNEVYYPVPFHRQECFGYLGEKDTDFPNANAQAEQSLAVPIYPELTKEQISE
                     VVNTITEFVKA"
     gene            520..1293
                     /gene="rfbF"
                     /locus_tag="PK754_02530"
     CDS             520..1293
                     /gene="rfbF"
                     /locus_tag="PK754_02530"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HNB08410.1"
                     /translation="MKVVILAGGYGTRISEESHLKPKPMIEIGGKPILWHIMKIYSGY
                     GYNDFVICLGYKGYVIKEYFANYFLHESDVTFDFRAHNAPVIHTHTAEPWRVTLVDTG
                     LDTMTGGRIRRVASYLDGERFFLTYGDGVADVDIRKLLSFHMTHGKTATVTAIQPSGR
                     FGAMAMDSSGMVQSFQEKPKGDGAWVNAGFFIFENSVLQLLKDDQTVLEREPLETLAR
                     SQSLVAFKHDRFWQPMDTLRDKNMLDELIREKRAPWVIW"
     gene            1278..2369
                     /gene="rfbG"
                     /locus_tag="PK754_02535"
     CDS             1278..2369
                     /gene="rfbG"
                     /locus_tag="PK754_02535"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HNB08411.1"
                     /translation="MGDLVKNNFSDIYRDKRVLVTGDTGFKGSWLSVWLHTLGAEVYG
                     FALPPKSNNDNFVRCDLQNKIHHRDGDIRDPHALADYVKEVRPDIAFHLAAQALVLDS
                     YRDPAETFETNVVGTVRFFEAVRAISSVRAAINVTSDKCYQNNEWVWGYRENDPMGGA
                     DPYSASKGASELITSSYAQSFFSSEGTCSIASARAGNVIGGGDWAENRIIPDFFRAVQ
                     KNQTLILRNPNAVRPWQFVLEPLRGYLTLGEKLFLHGKTYSGGWNFGPAMHHACSVQE
                     LILALISKTGFGRFETAAVKQNEHEAYMLRLDISKAVSRLSWKPALSLEQTIALTAQG
                     YHDDAFAGSLWDARKKQLDEYLSLSSHTI"
     gene            2426..3781
                     /gene="rfbH"
                     /locus_tag="PK754_02540"
     CDS             2426..3781
                     /gene="rfbH"
                     /locus_tag="PK754_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HNB08412.1"
                     /translation="MDQNVKAAAEKLRLEILEKTAEYYRLTHGQKKEFVPGQSAISYA
                     GRVFDEKEMQNAVDASLEFWLTSGHFSKEFESKLAKKLGVKHAMLCNSGSSANLLAFM
                     GLTSPLLEDRRIRKGDEVITVAAGFPTTIAPMLQFGAVPVFLDVHTETANIDTRALEA
                     AYSPKTKAVMIAHTLGNPFDLDAVTAFCKKYNLWLVEDNCDALGSEYRGRWTGTFGDV
                     ATSSFYPPHHITMGEGGAVYTNKSILKRSIESFRDWGRDCWCDSGKDNTCGKRFDWEL
                     GTLPCGYDHKYTYRHIGYNLKATDIQAAIGCVQLDRLEDFADRRRANWNYLKNGLAEF
                     SDIFILPEATPNTNPSWFGFLISLRSESGISRPELIHYLENKKIQTRMLFGGNLIRQP
                     FFDAFREQPGSYRVVGDLTNTDLIMNNGFWLGVYPGLTTPMLDYMIDAIRSFLRRDTV
                     S"
     gene            3781..4824
                     /locus_tag="PK754_02545"
     CDS             3781..4824
                     /locus_tag="PK754_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019942019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNB08413.1"
                     /translation="MHSIIREDIAHIISAPLPWDILRGKTVLISGANGFLPAYMVETL
                     LGLNDRLDMNIHVIGIVRSEERARQRFAHHAGRNDFKLHVADVTRLVFRSDTPVHFVV
                     HAASQASPKYYGIDPVGTLTANTTGTQNMLEVARQSQSERFLYFSSSEVYGQLEARQI
                     PTNEMQLGCLDSMALRSCYAEGKRLGETMCVAWSHQYGLSTVIVRPFHTYGPGMALDD
                     GRIFADLVSDIVNGKNIVLSSSGAATRSFCYLRDAIIGFFTVMLHGKTTEAYNVGNPE
                     CEISIRGLAEKLVTLFPEKKLSVVLDIPPQNGYIPSAVQRSVPDITKIKSLGWQPTTT
                     VEEGFTRTIKSYT"
     gene            4821..5747
                     /locus_tag="PK754_02550"
     CDS             4821..5747
                     /locus_tag="PK754_02550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNB08414.1"
                     /translation="MKITDIQNQYRSGAIKKSEYIQAMYEKHNQLFEYTDILRHTDIE
                     NITITSHGIVWTTRPHRLRWLTPKADKRSAPFEILNFAQYEPEYFSMMCHLLEPGMSV
                     FDIGANIGWYSLNFSKAVSDLHVCAFEPVPGTYLELENNIQLNTGMRVKSYNLGLSDR
                     AGKLTFFVDPELSTSASAVNITEAANAHETECALETMDNFCKQYGAKPDFIKCDVEGA
                     ELMVYRGGKYTLQTHKPIVCTEMLRKWAAKYNYHPNDIITFFKEMGYQCFFIQNDYLA
                     PITFVDETTVETNFIFLHRERHADKIKTWVKS"
     gene            5741..6550
                     /locus_tag="PK754_02555"
     CDS             5741..6550
                     /locus_tag="PK754_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNB08415.1"
                     /translation="MVTTDEVKAFSKAIRIEALKMVHASNSSHLGGAFSMADILAVLY
                     GGVMNVNPKNPRWPERDRFLLSKGHACSALYAALALRGFFDMSQLTSFAQNGSFFTSH
                     ANHHIPGVELSTGSLGHALSVGCGLALAAKRRGQAWRVFTLLSDGELDEGSNWEALLF
                     APHHHLNNMVVIVDHNKIQSLGHVNEVLDLGPLSEKFKSFGWYVTVTDGHDHAALSAA
                     ITKKTDDKPHIIIAETVKGKGVDFMEEKLLWHYRTPDAEQFRNALEQLEHS"
     gene            6550..7452
                     /locus_tag="PK754_02560"
     CDS             6550..7452
                     /locus_tag="PK754_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNB08416.1"
                     /translation="MRTAFIQQLMSEAKRDERVFLLVGDLGYSVVEPFAESFPDRFLN
                     IGVAEQNMAGIAAGLAKEGYIPFIYSIGNFPTLRCLEQIRYDICYHALGVKIVSVGGG
                     FAYGALGASHHGTEDLGILRTLPSMIVTAPGDPQEARLITTHAVQTDAPMYIRLGKAG
                     EPAVHPGNIAFAAGKMIPVCHGKNTVVLTTGAMLRYASDFIRHNNLLWGLYSTPYIKP
                     IDSATIELLAHSYERIITLEEHQRSAGLGSAVLEALHDCREKKKSMPIPLVHRIAIPD
                     QFIQIAGSQDYLRQSCGLVLSEYI"
     gene            7463..8773
                     /locus_tag="PK754_02565"
     CDS             7463..8773
                     /locus_tag="PK754_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNB08417.1"
                     /translation="MSAPPSSIPMTLRQLVERIKQSGYLKNVSRLSIGTIIGQAIAVL
                     LLPIVTRLYDSADYGIAATLNAMVMIASSTIMLRMDSAIVLADSDDQAAKIIRFCGQL
                     ILGFAIVVAISAVVFYGLALDLGFFPKGGPLYFWLPVWVILGAGNILLASWANRYAAY
                     RVLSFSRALPPLLAFIVMLSMYSWQGSTITGLVLGHISAAITLYITIFFGLRHEGKPI
                     RHLAVDWADIRNLLKRFKQFPTYGLLMTFLDQLTASLPLLIFTASFSPHDAACFALAT
                     NVLRLPSTVIGQGVAQVFYEMSAKLSEDATLLRSFVINNIKFLSFFSFPFLIIIVAAG
                     PWLFEWVFGAAWRDAGEYARSLVIAIAVNLVASPVSMISSVIRKQRMHFVISLLSFAI
                     RAAMIGIGVTSGSAYTTVILYSIGEAMMLIVFLIWVVRSVRMRA"
     gene            8773..9894
                     /locus_tag="PK754_02570"
     CDS             8773..9894
                     /locus_tag="PK754_02570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNB08418.1"
                     /translation="MKNNIILSIGVPTWNRSGFLKALLESIIHQAEKFDFTRSVEIIV
                     SDNCSTDDTGVVVKAMSAQTNVAIRYVRNSENIGAIRNMIQTFHQSRGRFWMAFGDDD
                     ILAEGILPEIMHHLTTRDDFSVVMFDQWKLIGHESLMTLESAAERYFYYMGNAGVFAI
                     RTEHARQGLKPGDDALIAQSYWPQTHIAFLAMKSGNLSKPFLSLPLISSYSPNHTENT
                     LYSGWYLWETGIRSLQHVALILKPELGTSFFRAVCRHLFWTRRLFFYLKNVMLYTAFF
                     DSPRQIRLLRENVRRHLRTISMCNGLALVMFFVLIELPPSVKRVLYSFIFMMLKPRQW
                     PHAKRDILVKKAIHERAHAAENEKGVPRSYDRDVQPEAS"
     gene            9919..11787
                     /gene="asnB"
                     /locus_tag="PK754_02575"
     CDS             9919..11787
                     /gene="asnB"
                     /locus_tag="PK754_02575"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNB08419.1"
                     /translation="MCGIAALINTKPTDGALIQRMTDIVSYRGPDGDGHNGFDGNRVW
                     LGQRRLAIIDLSNAGREPMSYKNERYWITYNGEVYNYIELRRELEAVGYVFASKTDTE
                     VVLAAYDYWGTECLHRFNGMWAFVLYDTLTKRTFVARDRFGVKPLYYYKTTDGGYAFA
                     SEIKQFSVLPGWRYSMNRQRVADFLGYAISDHTDETMYDRVLQLRGGECAEITYEAQT
                     VRFEKRRWYSFPDTQFDGTYEDATKQFLNVFTDAVRLRLRSDVPVGSCLSGGLDSSSI
                     VCLLHDMLSTQNTTALQKTFSAYAREQAYDESRYAEEVIRAIRVEAHHVYPDFETLFD
                     TLDTLLWHQDEPFGSASIYAQWHVFRLAKSGGVTVMLDGQGSDEQLAGYYPFYGTRLA
                     ELMVRGRWIRLISEIQKTIGEGHVNRGAVLRRLIGALIPNAWMPFMPSDSQSSNDWFD
                     MEKLGARSIHWRQQYGEFKPDVNTALRVQLTRTNLPMLLHWEDRNSMAHSIEARVPFL
                     DYRLVEFILSLPSDHKISNATTKRVLRDSMKGYVPESIIQRRDKMGFVTPEEHWVRTH
                     PDVFRKQLSSSIERSGGILRSSLRDDFDAMIAGKKAYDGRYWRGIVFGRWVRSDSM"
     gene            12224..13450
                     /locus_tag="PK754_02580"
     CDS             12224..13450
                     /locus_tag="PK754_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HNB08420.1"
                     /translation="MNNSNNTNDSGIEGQAAFPKSLGDWMEKLYPYRKKIIVTSFFIT
                     VATVAFLFLTKPWYESQAVILLPDKSSSAIESLGGALGGIGQTLLSGSGISGPQRYLA
                     ILRSQRLKEAIIARFKLIDFFEMEPTRKDTSALLELMEESIFFESNVKNSTISIQVRF
                     QGSPEMTADITNFAVSELDRINRELSTDQARFSRQFIEKRYYDSRIELKKAEDSLNFF
                     QKRHGIIALTEQTKASIETAADIQAQLVTGEAEYNIIRKSVGDNHPDAVRQKSRLDEL
                     RRIQNKMDVGGFNQSILIPFNSTADIALQYLRLFREVQINQKIVEYLIPQFEQTKIQE
                     AKDTPTVQVLDYGKPAAYPVKPKKKIITIIVGFLSLVLTSLFYVVNDIIRVSDMSKQN
                     RVTAIFKAFHLKGSTK"
     gene            13490..14464
                     /locus_tag="PK754_02585"
     CDS             13490..14464
                     /locus_tag="PK754_02585"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNB08421.1"
                     /translation="MDKVLITGAGGFIGSHLVELCVASGFSVKAFVHYNSKNEWGWLD
                     TLEEKANLEIVSGDIRDYDSVYSALEGCVGVFHLAALIGIPYSYVSPKAYIDTNIIGT
                     YNVLQGSKCLGLDKIIITSTSETYGTAQYVPINESHPLVGQSPYSATKIGADQLAISY
                     YRSFNLPVTIVRPFNVFGPRQSARAVIPTIVSQLLSGRRSIKLGSLNPTRDLTFVKDT
                     AAGFVEAFRSQTLKGETVNIGMGFEISIGDLAKKIGKLMQVEFEFESDSVRIRPEHSE
                     VERLWCDNTKLLKNTNWSPKYNLEIGLKETISFIEKNLNIYKPEIYNV"
     gene            14457..15617
                     /locus_tag="PK754_02590"
     CDS             14457..15617
                     /locus_tag="PK754_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HNB08422.1"
                     /translation="MSKVFFDEVVDFIRSWYDTDQPIPLHAPVFHGNERKYVLETIDS
                     TFVSSVGEYVTKFESMVADYTGATEAVATSNGTVALQIALLVSGVRHGEEVITQPLTF
                     VATSNAIAHVGAIPIYVDCSLSTMGLSPDSLNEFLETNAINIKGESYNRKTGRRLAAC
                     IPMHTFGHVVEIENIRVICDRFNIPLIEDAAESLGSYCGDTHTGLIGDVGVLSFNGNK
                     TITTGGGGMIITKNKQFALRAKHLTTTAKRPHQWEFFHDEVGYNFRLPNLNAALGCAQ
                     MERLPDLLRDKRELAYAYRDFFKDTKVRFFEEREGTTSNYWLNSIILSDRAERDEFLS
                     FTNKKNIATRPIWTLMNKLPMYQKCQTTPLPNAEWFEDRVVNIPSSPRVKNV"
     gene            15610..16251
                     /locus_tag="PK754_02595"
     CDS             15610..16251
                     /locus_tag="PK754_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HNB08423.1"
                     /translation="MSEKIKLVLVGGGGHCRSCIDVIETLEGVDILGVIDTDPSRSSI
                     GNYPILGDESTQNLDRLNPDYFLITVGQTKSCEKRKSIANTLKQHNKLFATIISSLAH
                     VAHNSFISQGTIVMHRVVVNANVHIGINCIINTGAIIEHDVIVGDFCHVSTGAIVNGN
                     CKIGKETFIGSGAVIKHGVSVCDCTIIGAGSVVVSDILESGTYVGVPARRLYE"
     gene            16244..17248
                     /gene="neuB"
                     /locus_tag="PK754_02600"
     CDS             16244..17248
                     /gene="neuB"
                     /locus_tag="PK754_02600"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HNB08424.1"
                     /translation="MNKVLIIAEAGVNHNGELRLAKKMIRAAAQAGADVVKFQTFRTE
                     SLVTTKAPKAKYQKKRDNADESQFQMLKRLELPYSKLDILQKECERNRVLFLSTAFDL
                     ESLDALHKMKLPFFKIPSGEVTNYLYLKKIAQFGRPVILSTGMCTLDEVEKAIRLLEK
                     NGIRRSFITLLHCNTEYPTPYRDVHLRAMQTLRSEFGVNVGYSDHTMGTEVAIAAVAL
                     GATVIEKHFTLDCGMSGPDHKASIEPRDFKRMVDAIRHIETALGRSTKKPSASEKKNI
                     KIARKSIVASQDIKKGDFFSEKNITVKRPGTGISPMQWNRVIGRQAKKDFNLDDLISI
                     "
     gene            17245..18423
                     /gene="neuC"
                     /locus_tag="PK754_02605"
     CDS             17245..18423
                     /gene="neuC"
                     /locus_tag="PK754_02605"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013876224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HNB08425.1"
                     /translation="MKKQKRHKLCVVTGARSEYGLLRPLIFALSKSKNFDLCVLVTGM
                     HLSEGFGMTESEIESDGFEIHERVEMLLDSMSQIGVSKSIGVGMIGLSDALSRLNPDA
                     MIILGDRFEAFAAAATAHVLRIPIIHLHGGELTEGSIDDGFRHAITKMSSLHFVSTEV
                     YRDRVIQLGEHPERVFHVGAIGIESIRSLKLKSEKELCRELNLPTLGNSLIITYHPTT
                     IANGNVEKECNELFHALEKFNNMTMIFTGSNADADGQRINKLMQQFVGKKYKQRRFFQ
                     SLGQLRYLSLLRFVKGVVGNSSSGIIEAPEFGVGTVNIGSRQDGRVKPRSVVDCACKR
                     SDIEMAIRKIISPEFSTVLDALDHPYGKGKTTEAIMDVLKSTQFSKLNRKKFFDIKKH
                     "
     gene            18426..19493
                     /locus_tag="PK754_02610"
     CDS             18426..19493
                     /locus_tag="PK754_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNB08426.1"
                     /translation="MKESIDQFFIDPTVTVRDAMKKLNSTGRKVLFVRTSQGTLFGSI
                     TDGDIRRWILKGGQLEAKASEICNITPHVVESNYDIEYVKSIMVRENIESLPVVTSDG
                     CVEKLLFWEEVFAKANVRRSGFSIDIPVVIMAGGKGTRMDPFTRVLPKPLIPIADKTI
                     LEFIFDEFVQFGVVRFFLTLNYKGELIEAYVKSLNKSYQVDCIWEKDFFGTAGSLTLV
                     RNHIESDFILSNCDNIVKADYHEVLEFHRQNNAWLTILSSIQNHRIPYGVVSFEDGGT
                     VNRIIEKPEYSFHVNTGVYIINRKALDYVPDHTIFHMTDLIDRLLLDKRKVMTYPVTE
                     NDYIDIGQWDEYKKAVEKLKI"
     gene            19496..20212
                     /locus_tag="PK754_02615"
     CDS             19496..20212
                     /locus_tag="PK754_02615"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HNB08427.1"
                     /translation="MSYKEKSILVIIPARGGSKGVPAKNIRPLAGKPLIAYTIEAATK
                     SKYVDRVIVSTDSETIADTCKMYGISIPFIRPSELSTDESPTWQTVEHAVDWLSNNEK
                     KTYDVICLLQPTSPLRNVRHIDQSISKFLSCNADSLVSVCRSPKSPYWSQHITPDGYL
                     SPLFPDSGNYIRRQDVPVTFDLNGAIYLVKTEFFRRTHLFKGPRTVAYEMDYQSSIDI
                     DTPLDFEIAEIIRKQLGSHE"
     gene            20205..21050
                     /locus_tag="PK754_02620"
     CDS             20205..21050
                     /locus_tag="PK754_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase cyclase
                     subunit"
                     /protein_id="HNB08428.1"
                     /translation="MNKRIIARLDIKGPNLVKGIHLEGLRVLGDPSKFAHSYFHEGAD
                     ELIYQDVVASLYDRNSLHSFIAKAAEQILIPLTVGGGIRTIEDIRTILRFGADKVSLN
                     TGVLKRPELIREAANSFGSSTIVIAIEAIRQKDGKYYCYIDNGREFSGREVADWVCEV
                     EALGAGEILLTSVDRDGTGLGFDIELVKMVSGLVNIPVIAHGGCGKIEDISEVFKVGN
                     ADAVAVASVLHYGMIGKEVRALHDITEGNFEFLKSNKSFSKITPLSMLEIKKQLIDLG
                     YDIRN"
     gene            21055..21729
                     /gene="hisH"
                     /locus_tag="PK754_02625"
     CDS             21055..21729
                     /gene="hisH"
                     /locus_tag="PK754_02625"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012992061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNB08429.1"
                     /translation="MKTQVSHRVSVCIIDYHLGNLFSVKRACELIGLRPIISSDPNVI
                     RNSDALILPGVGAFADAMRNLKNMKIDVAIKDHINDHKPFLGICLGLQLLFSESHEFG
                     IHQGLGVIQGKVVRFQNENRKLKVPQVGWNFIKKTGGLPDVVWGKTPLFDIDIKQRMY
                     FVHSYYVVPEDKSVQLTETEYEGVKYCSSVFTKNIFACQFHPEKSSYEGLKIYKNWAD
                     FLEHKD"
     gene            21733..22962
                     /locus_tag="PK754_02630"
     CDS             21733..22962
                     /locus_tag="PK754_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008336841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HNB08430.1"
                     /translation="MRNKNLEAYYGLPNNVFFCKRCVMSNQRPTSTIEFRHIAGSKKN
                     TMNFDDKGICDACRHAEIKKTIDWKKRESELLRLLEKYRRSDGNYDCLVPGSGGKDSA
                     YQAHILKTKYGMNPLTVTWSPIMYTDYGYQNWKNWIEVGGFDNLLFKQNGKAMKLLTK
                     LSIVNLLHPFQTFILGQKNLAPKVAAKFGISLIFYGENEAEYGNPIADNVSSLRDKSY
                     YSFSNLNEVFLGGVCVPDLISNYGLTLNEIMPFLPATSDELKRCEVEVHYLGYYLKWT
                     PQEVYYYAVENTNFTARPFRTQGTYSKYNSIDDKIDDLHYYTTYIKFGIGRATYDASQ
                     EIRNDHITREEGQALVRKFDGEFPDRYFEEIMRYLDINPSDFHMLCDKFRSPHLWAQV
                     NGEWKLRHTVNSDGVDD"
     gene            23001..>23410
                     /locus_tag="PK754_02635"
     CDS             23001..>23410
                     /locus_tag="PK754_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08431.1"
                     /translation="MSLYQKKINWRSLIINTIRLDIYDLNAINSNQAMRYYDLLPQSQ
                     LPRKGIVARMASFYVNMYKSFVTCLQTQRLDDLLECEQMAFVTTRNQKEAIRSVISFS
                     GDTHCYGIHGHGDISFDLFKAYLLAIPFFPLVMY"
BASE COUNT         6686 a         4364 c         5546 g         6814 t
ORIGIN      
        1 gtaaaccaaa atattatcac aaaatgatcg gcggtaattt tcgccttgat gcgattcagg
       61 ccgctgtcct taatgtaaaa cttccgcatc ttgataactg gagccaacgg cgtcgtgaaa
      121 atgccgatct gtatacccaa ctttttattg cagccggagt ggctgaagcg gaaggtaaga
      181 taacttacga cagcaaaaat aaagtactgc ttcccaaagc cgcataccgc acgtccggag
      241 cgaaaaacca ccatatctat aatcaatatg taatacgagt agaaaaacgt gatgcattgc
      301 gtaaatatct aaccgataaa caaatcggca acgaagtgta ttacccggtg ccgtttcatc
      361 gtcaggaatg ttttggttat ctcggtgaaa aagataccga ttttcccaat gccaatgccc
      421 aagcggaaca atcgcttgcc gtgccgattt atccggagct cacgaaagag cagatttcgg
      481 aagtagtcaa taccatcact gaattcgtga aagcttgata tgaaagttgt cattttagcc
      541 ggcggttacg gtacgcgcat cagtgaagag tcgcacttaa aaccgaaacc gatgatcgaa
      601 atcggtggta aaccgatttt atggcacatt atgaaaatct actccggcta cgggtacaat
      661 gattttgtga tatgcctcgg ctacaagggc tatgtgatca aagaatattt tgccaattat
      721 tttttacacg aatcggatgt cacgtttgat tttcgtgcgc acaatgcgcc cgtcattcac
      781 acgcatacgg cagaaccgtg gcgtgtgacg ttggtggata ccggactgga taccatgacc
      841 ggcggacgta tcagacgcgt tgcttcctat cttgatggcg aacgtttttt tctgacttac
      901 ggcgacggag tggccgatgt ggatatccgt aaactcttgt cattccatat gacgcacggc
      961 aaaacggcga cagtgacggc gatacagccg agcgggagat tcggtgccat ggctatggat
     1021 tcgtccggaa tggttcagag ttttcaggaa aaacccaaag gcgacggggc ttgggtgaat
     1081 gccggttttt ttatttttga aaacagtgtt ttacaattac tcaaagacga tcaaaccgta
     1141 ttggaacgtg aaccgttaga aacattggct cgttcacaat cgcttgttgc attcaaacat
     1201 gacagatttt ggcagccgat ggatacattg cgtgataaga atatgctcga cgaacttata
     1261 cgtgaaaagc gcgcaccatg ggtgatctgg tgaaaaacaa tttttccgac atctatcgcg
     1321 ataaacgcgt tttggttaca ggcgataccg gtttcaaagg atcttggctc agtgtttggt
     1381 tgcataccct tggcgccgag gtttacgggt ttgctctccc tccgaaatcc aataacgata
     1441 atttcgtacg atgtgatttg caaaataaaa tacatcaccg cgatggtgat attcgcgatc
     1501 cgcacgcatt ggcagattat gttaaagaag ttcgacccga tatcgcattt catcttgccg
     1561 cccaagcgtt ggtactggac agttatcgcg atcctgcaga gacatttgaa accaacgttg
     1621 tgggcacggt tcgttttttt gaagcggtgc gcgcaatatc atccgttcgt gcagcaatca
     1681 atgtaactag cgataagtgt tatcaaaata acgagtgggt gtggggttat cgtgaaaacg
     1741 atcctatggg tggcgccgac ccctatagcg cttccaaagg cgcatcggaa ctgatcacgt
     1801 cttcatatgc gcaatctttt ttttcgagcg aagggacgtg ctccatcgct agtgcgcgtg
     1861 ccggtaatgt aatcggcgga ggggattggg ccgagaatcg catcattcct gattttttca
     1921 gagcggtaca aaaaaatcaa acactgatac tgcgaaaccc caatgcagtt cgtccgtggc
     1981 aattcgttct tgagccgttg cgcggctatc ttacgttggg ggagaaattg ttcctccacg
     2041 gtaaaacata ctccggcgga tggaattttg gaccggcaat gcatcatgcg tgctcggtac
     2101 aggaacttat cctcgcgctg atttccaaaa caggtttcgg acgatttgaa accgcggcgg
     2161 taaaacaaaa tgaacacgaa gcctacatgt tgcgtttaga tatatccaag gctgtttcac
     2221 gattgtcgtg gaagccggct ttatcactgg aacagaccat tgcattgaca gcccaaggct
     2281 atcacgacga tgcattcgcc ggttcgcttt gggatgcacg taaaaaacaa ctcgatgaat
     2341 atctaagctt gtcatcccac acgatctgaa gatttgattt tttaactttg agcgcgactc
     2401 cgaaaacgag aacaaaataa acgacatgga tcaaaacgta aaagccgctg cggaaaagct
     2461 tcggctcgaa attttagaga aaacggccga atattaccgt ttgacgcacg gtcaaaaaaa
     2521 ggaatttgtg cccggtcagt ccgctatatc gtatgcggga cgcgtgtttg atgaaaaaga
     2581 aatgcaaaac gccgtagatg caagtcttga gttttggctt acatccgggc atttttcaaa
     2641 ggagtttgaa agcaagctag ccaaaaaact cggcgtgaag catgctatgt tgtgcaactc
     2701 cggttcgtcg gccaaccttt tggcgtttat ggggttgaca tcaccgttac tggaagatcg
     2761 tcggattcgt aaaggcgacg aagttataac cgtagcggcc ggtttcccta cgacgattgc
     2821 tccgatgttg caatttggcg ccgtacctgt ttttcttgat gtgcataccg aaacagcgaa
     2881 tattgatacg cgagcattgg aagcggcgta cagcccaaaa acaaaagcgg tcatgatcgc
     2941 tcatacgcta ggcaaccctt ttgatctgga tgccgtgacg gcgttttgta aaaaatataa
     3001 tctatggctc gtagaagata attgcgatgc gctgggttcg gaatatcgtg gccggtggac
     3061 aggaacattc ggtgatgtcg cgacaagtag tttttatccg ccgcaccata ttacgatggg
     3121 tgaaggcggc gccgtgtata ctaataaaag tattctgaaa cgcagtattg aatcatttcg
     3181 tgattggggt cgtgactgct ggtgcgattc cggaaaagat aatacgtgtg gtaaacggtt
     3241 tgattgggaa ctcggaacgt tgccgtgcgg ttatgatcat aaatacacgt accgccatat
     3301 aggttacaat cttaaagcta cggatattca agccgcaata ggttgcgtgc aattagacag
     3361 acttgaggat tttgccgata gacgacgtgc aaattggaat tatctgaaaa acggtttagc
     3421 ggaattctcg gatattttta ttttacctga agccacaccc aataccaatc cgagttggtt
     3481 tggttttctc atcagtcttc gcagcgaaag cggtatttct cgtcccgagt tgatccacta
     3541 tctagaaaac aaaaaaattc aaacacgcat gttgtttggc ggcaatctca tacgccaacc
     3601 tttttttgat gctttccgag aacagccggg ttcttaccgc gttgtaggtg acttgaccaa
     3661 tacggatctt atcatgaata acgggttttg gttgggggtg tatcctggtt taacgacccc
     3721 gatgttagat tatatgatcg acgctatccg ttcatttctc cgacgagata cggtttcgtg
     3781 atgcattcaa taatacgcga agacatcgcg catattattt ctgcgccgct tccttgggac
     3841 atactccgtg gaaaaactgt tcttatcagt ggggccaatg gttttctacc ggcgtatatg
     3901 gtagaaacgt tattaggact caatgatcgt ttggatatga acattcatgt aatcgggatt
     3961 gtacgatccg aggaacgtgc acgtcagagg tttgcacatc atgccggacg taacgatttt
     4021 aagttacacg tagccgatgt gacacggttg gtatttcgct ctgatacgcc ggttcatttc
     4081 gtagtacatg ccgcaagcca agcctcgcca aaatattatg gtatagatcc ggtgggtact
     4141 ttgacggcca atacgaccgg cacgcaaaac atgcttgagg tggcgcgtca atcccaaagc
     4201 gaacgatttt tgtactttag cagcagcgaa gtatatggtc aactggaagc gcgccaaata
     4261 ccgaccaatg agatgcagct tggttgcttg gacagtatgg cgctgcgttc atgttatgcg
     4321 gaaggcaaaa ggcttggtga aacaatgtgc gttgcatggt ctcaccagta tggtctttcc
     4381 acggtgatcg ttcgaccgtt tcacacctat gggcccggca tggcgctgga tgacggtcgt
     4441 atttttgcgg atttggtttc agatattgtt aatggaaaaa atattgtact tagtagtagc
     4501 ggtgctgcaa cgcgctcctt ttgttatctt cgcgatgcga ttatcggttt ttttacggtt
     4561 atgctccacg gaaaaacaac tgaagcatat aatgtaggta accctgaatg tgaaatcagc
     4621 attcgaggat tggcggaaaa attagtaaca ctgtttccgg aaaaaaaatt atcagtggtt
     4681 ttggatatcc cgccgcaaaa tggctatatt cccagcgccg tacaacgcag tgttccggat
     4741 attaccaaaa taaagagctt aggttggcag ccgacgacaa ctgtcgaaga gggttttaca
     4801 cgaacgatta agtcctatac atgaaaatta cagacattca aaatcaatac cgttccggtg
     4861 cgattaaaaa atcggaatac atacaagcga tgtatgagaa acataatcag cttttcgaat
     4921 acacggatat actccggcac acggatatcg aaaatatcac gatcacgtcg catggtatag
     4981 tatggactac gcgtccgcac cggcttcgtt ggttaacccc aaaagccgac aaacgcagtg
     5041 cgccgtttga aattcttaat tttgcgcagt acgaaccaga gtatttttcc atgatgtgcc
     5101 atttgttgga accgggtatg tcggtttttg acattggcgc taatatcggc tggtattcac
     5161 taaatttttc taaggccgtt tccgatttgc atgtttgtgc gtttgagccg gtacccggaa
     5221 cgtatctgga gctggaaaat aatattcagc tgaatacagg catgcgtgtt aagtcgtata
     5281 atctcggctt atccgatcgc gcaggaaaac ttacgttttt tgtcgatccg gaactttcga
     5341 ccagtgcatc ggctgtcaat ataaccgaag ccgctaatgc acacgaaaca gaatgtgcgc
     5401 tggaaacgat ggataatttt tgtaaacagt atggtgcgaa gccggacttt atcaaatgcg
     5461 atgtggaagg cgcggaattg atggtctatc gtggagggaa atacacgctg caaacccata
     5521 agccgatcgt atgtacggaa atgttgcgta agtgggcggc aaagtacaat tatcatccta
     5581 atgacatcat tacttttttt aaggaaatgg gataccaatg tttttttatt caaaatgatt
     5641 atctcgcacc gataaccttt gtggacgaaa cgaccgtcga aaccaatttt atttttttgc
     5701 atcgtgaacg gcacgccgat aaaatcaaga cttgggtgaa atcgtgacca cggatgaagt
     5761 aaaagcattc agcaaagcga ttcggatcga agcgttaaaa atggttcatg cttcgaattc
     5821 gtcgcacctt ggcggcgctt tttccatggc ggatatttta gctgttttat acggcggtgt
     5881 gatgaatgtc aatcccaaaa acccacggtg gcctgagcgg gatcgttttc ttctcagtaa
     5941 agggcacgcg tgctctgctt tatatgcagc gcttgcgttg cgcggttttt ttgacatgtc
     6001 acaacttact tcctttgcgc aaaatggaag tttttttact tcgcatgcca atcatcacat
     6061 cccgggcgta gaattgtcca cgggaagttt ggggcacgcg ttatcggttg gttgcgggct
     6121 ggcgttggcc gccaaacgac gcgggcaagc gtggcgtgtt tttacgctcc ttagcgacgg
     6181 agagttggat gaaggatcca attgggaagc attgctgttt gcaccgcacc atcatctgaa
     6241 caacatggtg gtgatcgtag atcataataa aatacaaagc cttggtcatg tgaatgaagt
     6301 gcttgacttg gggcctttat ctgaaaagtt caaatccttt ggatggtatg tgaccgtaac
     6361 ggatggtcat gatcatgccg cgttatcggc ggcgataacg aaaaaaacag atgataaacc
     6421 gcatatcatt attgctgaga cagtcaaagg caagggtgtt gattttatgg aagaaaaatt
     6481 gctttggcac tatcgtacac ctgacgctga acaattccgt aatgcgctgg aacaactgga
     6541 gcattcgtaa tgcgtacggc ctttattcag caactgatga gcgaagcaaa acgtgatgaa
     6601 cgcgtatttt tgttggtagg tgatctcggc tattcggtgg ttgagccgtt tgccgagtcg
     6661 tttcctgatc ggtttctcaa tatcggtgtg gccgaacaaa atatggccgg tattgccgca
     6721 ggtttggcca aagaaggata tattccgttc atttattcga ttggtaattt tcccacgttg
     6781 cgatgcttgg aacagattcg ctacgatatt tgttaccatg cgcttggtgt aaaaatcgta
     6841 tcggtcggcg gaggatttgc atacggtgcg cttggggctt cacatcatgg aacggaagat
     6901 ttaggaattt tacgtacgct tccgagtatg attgttacgg cgccgggtga tcctcaggaa
     6961 gcccgcctca tcacgaccca tgccgttcaa accgatgcac cgatgtatat ccgacttgga
     7021 aaagccggcg aaccggctgt acatcccgga aatatcgcat tcgcagccgg taaaatgatt
     7081 cctgtttgtc acggaaaaaa tacagtcgtg ttaacaacag gcgccatgct tcgttacgcg
     7141 tcggatttta ttcgtcataa taatttgctt tggggtttgt atagtacgcc gtatatcaaa
     7201 cccattgaca gcgcgactat agagctattg gcacattcgt acgaacgaat catcacgctc
     7261 gaagaacatc agcgtagtgc cggtttaggt tcggccgtcc tcgaagcttt gcatgattgc
     7321 cgtgaaaaga aaaagtccat gccgataccg ctcgtacatc gcattgcaat tcccgatcag
     7381 tttatacaaa tcgccggatc gcaagactat ctgcggcaat cttgcggact cgtattgtcg
     7441 gaatacatat agcgcaatta tcatgtccgc tcctccatca tccattccta tgacgctacg
     7501 ccaattggta gagcgcatca aacaaagcgg ttacctcaaa aatgtgtccc gattgtccat
     7561 tggaacgatc ataggccagg cgatcgctgt tcttttattg ccgattgtaa cacggcttta
     7621 tgattcagcg gattacggga tcgccgcaac actcaatgct atggtgatga ttgcgtcttc
     7681 cacaatcatg cttcgcatgg attcggcgat cgtcttggcg gactccgatg atcaggctgc
     7741 aaagatcatt cgattttgcg ggcaactgat tttgggtttc gcgatagtgg tcgctatcag
     7801 tgcagtggtg ttttatggct tggcgttgga tttgggattt ttccccaaag gcgggccgct
     7861 atatttttgg ttgccggtgt gggttatttt aggcgcaggt aatatcctgc ttgcgagctg
     7921 ggccaatcgt tatgcggcgt atagggtatt gagcttctcg cgtgctttgc cgccgctgtt
     7981 ggcgtttatt gtcatgctgt caatgtattc gtggcaaggt tcgacgatca cgggcttggt
     8041 attgggtcat atatcggcgg ccatcacatt atacatcacg attttttttg gattgcggca
     8101 cgaagggaag ccaatacgcc atcttgctgt tgattgggcg gatatccgaa atttgctaaa
     8161 acgctttaaa cagtttccga cgtatggttt gctgatgacg tttcttgatc aactgacggc
     8221 gtcattgccg ttgttaatat tcacggcatc gttcagtcct cacgatgcgg cctgttttgc
     8281 gttggcaacc aatgtgcttc gtttgccaag tacggtgatc ggacaaggtg tggcgcaagt
     8341 tttttatgaa atgtcagcca aactaagtga agatgcgaca cttttgcgtt cgtttgtgat
     8401 caataacatt aagtttctgt cctttttttc gttcccgttt ttgattatca ttgtggccgc
     8461 cggtccttgg ttattcgaat gggtttttgg tgctgcgtgg agggatgccg gcgaatatgc
     8521 ccggtccctt gtgatagcga ttgcagtaaa ccttgtcgcg tcacccgtaa gcatgatttc
     8581 gtcggtgatt cgcaagcagc gtatgcattt tgttatttca cttctaagtt ttgcgatacg
     8641 tgcggccatg atcggtatcg gtgtgacctc cggctcggct tataccaccg taatcttata
     8701 cagcatcggt gaagcgatga tgttgattgt ttttttgatt tgggttgttc gttcggtaag
     8761 gatgcgtgca taatgaaaaa taatataatt ctctctatcg gcgtaccgac atggaatcgc
     8821 tccggatttc tgaaagcgct gcttgagagt attatacatc aagccgaaaa atttgatttt
     8881 acccggtccg tcgaaattat cgtttcggat aattgttcta cggatgatac cggcgttgtc
     8941 gtaaaggcga tgagcgcaca aacaaatgta gcgattcgtt atgtacgcaa ttcggaaaat
     9001 atcggtgcca tccgaaatat gatacaaacg tttcatcaaa gccgcggaag gttctggatg
     9061 gcatttggtg atgacgatat tctggctgaa ggcatacttc ctgaaatcat gcatcatctg
     9121 acaacgcgtg atgatttttc tgtagtgatg tttgatcaat ggaaactcat aggccacgaa
     9181 agccttatga cgttggaatc ggccgccgag cgctattttt attatatggg taatgccggt
     9241 gtttttgcga ttcgcaccga gcatgcccgg caggggttaa aaccgggtga tgatgcattg
     9301 atcgcacagt cgtattggcc gcaaacgcat attgcttttt tagccatgaa aagtggtaat
     9361 ttatccaaac cgtttctttc gttgccgctc atcagttcgt attcgcccaa ccatactgag
     9421 aatacgttat attccgggtg gtatctgtgg gaaactggga ttcgttcatt gcagcacgtc
     9481 gcattgattt taaagcctga attagggacg tcattttttc gtgctgtttg ccgacattta
     9541 ttttggaccc ggcgcctttt tttttattta aaaaacgtca tgctatatac tgcgtttttt
     9601 gacagtcctc gccagattcg tcttttgcgt gaaaatgtgc ggcgtcattt gcgaacaatc
     9661 tctatgtgta acggattggc attagtgatg ttttttgtct tgatcgagtt gccgccttcg
     9721 gttaaacgag tattgtacag ttttatattt atgatgctca agccccggca gtggccgcac
     9781 gctaaacgtg atatcttggt gaaaaaagca attcacgaac gcgcacacgc cgcggaaaac
     9841 gaaaaaggag tgccgcgaag ttatgatcgt gacgtccagc cggaagcaag ttaattacaa
     9901 aacggagtat tttcagcgat gtgcggaata gcggcgctta tcaatacgaa gccgacggac
     9961 ggtgcgctga ttcaacgtat gaccgatatc gtttcatatc gcggaccgga cggtgacggg
    10021 cataatggtt ttgatgggaa tcgtgtgtgg ctcggccaac gtcgtcttgc catcatagat
    10081 ctcagcaacg caggccgtga accgatgagt tataaaaatg aacgttattg gatcacgtac
    10141 aacggggaag tatataatta catcgaattg cgtcgtgagc tggaggcggt aggatatgtg
    10201 tttgcatcca aaacggatac cgaagtggtc cttgcggcgt acgactattg gggaacggaa
    10261 tgccttcatc gtttcaatgg tatgtgggcg tttgttcttt atgatacgct gaccaaacgt
    10321 acttttgtag cgcgggatcg ttttggtgtt aagcccctgt attattataa aacaacggat
    10381 ggagggtacg cattcgccag cgaaatcaag cagttctccg ttttaccggg atggcgatat
    10441 tcgatgaata gacaacgcgt agccgatttt ttgggttatg cgatttccga tcatacggat
    10501 gaaaccatgt acgatagggt tttgcagctt cgtggaggag agtgcgcgga gatcacgtac
    10561 gaggctcaaa ccgtacgatt tgaaaaacgc cgttggtatt cttttccgga tacacagttt
    10621 gacggtacgt atgaggatgc gacgaaacag tttttaaatg tttttacgga tgcggtacgg
    10681 ttgcggttgc gctccgatgt gccggtaggt tcgtgtctct ccggcggatt ggattcgtct
    10741 tcaattgttt gtttgttgca tgatatgctg tccacgcaga atacgacggc attgcaaaaa
    10801 actttttcag cctatgcacg ggagcaggca tacgatgaaa gccgttatgc ggaagaagtg
    10861 attcgtgcca ttcgtgtcga agcgcatcat gtttatccgg attttgaaac gctgtttgat
    10921 acgttggata cgttgctgtg gcatcaggac gaaccgtttg gttcagcgag tatttacgca
    10981 caatggcatg tttttcgatt ggccaaatcc ggcggtgtaa cggtgatgct ggacggtcag
    11041 ggttcggatg agcagttggc cggttactac ccgttttatg ggacgcgttt agcggaactc
    11101 atggtgcgcg ggcgttggat tcgcttaatt agcgaaattc aaaaaactat aggcgaaggc
    11161 catgtcaacc gcggcgctgt cttacggcga ttgataggag cgcttatacc taacgcatgg
    11221 atgccgttta tgccgtcgga tagccaatcg tccaatgatt ggtttgacat ggaaaaactc
    11281 ggagcgcgtt caatacattg gcggcaacaa tatggagaat ttaaaccgga tgtaaataca
    11341 gcgcttcgcg ttcaattgac gcgtaccaat ttaccgatgt tattgcattg ggaggatcgt
    11401 aattcgatgg cgcattctat cgaagcgcgt gttccctttt tggattatcg gctggtggag
    11461 tttatcttat cgttaccgtc tgatcataaa atttcgaatg caacgaccaa acgtgtatta
    11521 cgtgacagca tgaaaggtta tgtgccggaa agtattattc agcgtcgtga caagatggga
    11581 tttgtaacgc cggaagaaca ttgggttcgt acacatccgg atgtatttcg caagcaactg
    11641 agttcttcga ttgagcgttc gggggggata ttacgatctt cgctacggga tgattttgat
    11701 gcgatgattg ccggtaaaaa agcttacgat gggcgatatt ggcgggggat cgtgtttgga
    11761 aggtgggtga gatcggattc gatgtaaaag gttgtatttg ttaggctttt ttattaaata
    11821 attactctaa ttggttaatg ctaaatttag tatcgcatat gaaaaattaa tcattcaata
    11881 aaatataagc attctctttt ttgaagaaaa aaagtcgtaa aatacgttaa atggatattt
    11941 ttttgaatta ggtcttaaat taagttggta gttggtatga gcgtacatca tttgatgttt
    12001 ttgtcagtta ttaacggact cataattgca tagacatctg cttacgcggg tgagacagcg
    12061 tgccgccgta ctgtcgttcc cgcgaaagcg tgaacgacag tacgaaacgg aaagctgtat
    12121 tcgcaataat gaatttgatc gattgacact gttgttacaa ttataaggcc gttaataact
    12181 cgctcatcgc tatgcatgtt ttattcttat ggtcagaaat atcatgaaca attccaataa
    12241 cacaaatgac tctggcattg aggggcaagc tgctttccct aaatcacttg gtgattggat
    12301 ggaaaaactg tatccgtatc gaaaaaaaat aatagtaaca tcttttttta ttacggtagc
    12361 aacagtagcg tttttgtttt taaccaaacc gtggtatgaa tctcaggctg ttattttatt
    12421 acccgataaa tcatcctctg cgattgagtc attaggtgga gcgttagggg gaataggtca
    12481 gacattgctt tcaggtagcg gaatttcagg tccgcaacga tatttggcaa tattgcgtag
    12541 tcagcgatta aaagaagcaa ttatagctcg atttaagctg attgattttt ttgaaatgga
    12601 gccaacaagg aaagacacat ctgctttact tgagttgatg gaggaatcta ttttttttga
    12661 aagtaatgta aaaaattcaa ctatttctat ccaagtgaga tttcaaggat cacctgagat
    12721 gacagcagat ataactaatt ttgctgtttc tgaattagat agaatcaatc gtgaattatc
    12781 aacggaccaa gcgcgattct cgcgccaatt tattgaaaaa aggtattacg attctcggat
    12841 agaattaaaa aaagcagagg atagcctcaa tttttttcaa aaacggcacg ggatcatagc
    12901 gctaaccgag caaacaaaag catccattga gacggcagca gatatccagg ctcaattggt
    12961 gacgggtgaa gctgaataca atataattag aaaaagcgtt ggcgataatc atccggatgc
    13021 cgttcgtcaa aaatcacgat tggacgaact tcgaagaatt caaaacaaaa tggatgtcgg
    13081 tgggtttaat caaagtatcc ttattccttt taatagtaca gccgatatcg cattgcagta
    13141 tttacggcta tttcgtgagg tacagattaa tcagaaaatt gtcgaatacc tcatccctca
    13201 atttgaacaa actaaaattc aagaagcaaa ggacacgcct accgtacagg tgttagacta
    13261 tggtaagccg gcggcatatc cggttaaacc aaaaaagaaa ataattacaa ttatagttgg
    13321 atttttatct ttggttctta catcgctttt ctatgttgtg aatgacatca ttcgcgtaag
    13381 cgatatgtcg aaacaaaatc gagtaactgc gatttttaaa gcgtttcatt tgaagggctc
    13441 tactaaataa tttattcatt gaagatttct ataactttag gtgttttgca tggataaggt
    13501 tctgattaca ggtgcaggcg gttttattgg ttctcattta gttgaattat gcgttgcctc
    13561 cggtttctcg gtaaaagcat ttgtacatta taattcaaaa aacgaatggg gctggcttga
    13621 tacgttagaa gaaaaagcaa atttggaaat agtttcgggt gatattcgag attatgattc
    13681 ggtttattca gcgctcgaag gctgcgttgg tgtttttcac ttagcagctt taataggtat
    13741 tccttattca tacgtttcgc caaaggcata tattgacacc aatatcattg ggacttacaa
    13801 tgttctacaa ggttccaagt gtctagggtt agataaaata attattacgt ctactagcga
    13861 aacctatggt acggctcagt atgttccgat taatgagtct catcctttgg taggtcaatc
    13921 accatattca gcgactaaaa ttggagcgga tcagttggcc ataagttatt atcgttcatt
    13981 taatttgccg gtaacgatag tgagaccatt caatgttttt ggtcctcgtc aatcagcgcg
    14041 tgcagtgata cctacgatag tatctcaact tctctctggg cgccggagta tcaaactcgg
    14101 aagtcttaat ccgacgcgcg atcttacttt cgttaaagat acggccgcgg gattcgttga
    14161 agcctttcga tctcaaacgc taaagggaga aacggtcaat atcgggatgg ggtttgaaat
    14221 cagtatcggt gatttggcaa aaaaaattgg caaattgatg caggttgaat ttgaatttga
    14281 atcagattct gtacggataa ggcctgagca cagcgaggta gaaaggctgt ggtgtgataa
    14341 tactaaatta ttaaaaaaca cgaattggag ccctaagtat aacttggaaa ttggcctaaa
    14401 agagactatc tcgtttatag agaaaaactt aaatatctac aaacctgaaa tatataatgt
    14461 ctaaagtatt ctttgatgag gtagttgatt ttataagatc ttggtatgac actgatcaac
    14521 caatacctct gcatgcacct gtttttcatg gtaacgagag aaagtatgta ttggaaacta
    14581 ttgactctac atttgtttcc tctgtaggag aatacgttac gaagtttgaa agtatggtgg
    14641 cagattatac aggggctacg gaggctgtag cgacctcgaa cggaaccgtt gctcttcaaa
    14701 tagcattatt ggtaagtgga gtgcgtcatg gggaagaggt aatcacgcaa cctttaactt
    14761 ttgtggccac aagcaatgca attgctcatg tgggcgctat tcccatttat gtagattgta
    14821 gtctatcaac gatggggttg agtccagaca gtttaaatga atttttagaa acaaacgcga
    14881 tcaacataaa aggggaatca tataacagaa aaacaggtag gcggttggcg gcgtgcattc
    14941 ccatgcatac ctttggccat gttgtggaga tagaaaatat ccgagtaatt tgtgatagat
    15001 ttaatatacc gctgattgaa gatgctgctg aatctttggg gagttattgc ggagataccc
    15061 atacaggact aataggggat gtaggagttt taagttttaa tggaaataaa actattacaa
    15121 ctggcggcgg aggaatgata ataactaaaa ataaacagtt tgcacttcga gccaagcatc
    15181 tcacgacaac agccaaaaga ccgcatcaat gggaattttt tcacgatgaa gtcggatata
    15241 attttcgttt acctaatcta aatgcagcgc ttggttgtgc acaaatggag cgtttacctg
    15301 atttattacg ggataagagg gagctggcgt atgcatacag ggattttttc aaggatacaa
    15361 aagtgcgttt ttttgaagag agagagggta caacatctaa ctactggcta aactcgatca
    15421 tattatcaga tcgggctgaa cgtgatgagt ttttgagttt taccaataag aaaaatattg
    15481 caacacgtcc gatttggaca ttaatgaata agttgccaat gtatcaaaaa tgtcaaacga
    15541 ctcctttacc aaatgcggaa tggtttgaag atcgcgtagt taatatacct agttctccaa
    15601 gagtaaaaaa tgtctgaaaa aataaaactt gtacttgttg gcggaggcgg ccattgtaga
    15661 tcatgtattg atgttataga gacactggaa ggggtggata ttttaggtgt aatagatact
    15721 gatccatcaa ggtcatcaat tggaaactat ccaatactag gtgatgaatc cactcaaaat
    15781 ttggatcgat taaacccgga ttattttctg attactgtgg gtcaaaccaa gtcttgtgaa
    15841 aaaagaaaat ctatcgcaaa cactctaaaa cagcacaaca aattattcgc gacgattatt
    15901 tctagccttg ctcatgtggc tcataattca tttatatctc agggtactat tgtaatgcac
    15961 agagttgtgg taaatgcgaa tgtacatata ggtattaatt gtattatcaa tactggagct
    16021 attatcgagc atgatgttat tgtaggcgat ttttgtcatg tttcgacagg cgcaatagtt
    16081 aatggtaact gtaaaatagg taaagaaacg tttattggaa gtggtgcagt tataaaacat
    16141 ggggtatcag tctgcgattg tacgattatc ggagcaggct cagttgttgt ttcagatata
    16201 ctagaatcgg gaacctatgt cggagtaccg gctaggaggt tatatgaata aagtactaat
    16261 aattgcagaa gcgggtgtaa atcataacgg agagctacgt ctggcaaaaa aaatgataag
    16321 agccgccgca caagcaggtg ccgacgttgt taaatttcaa acatttcgta ctgaaagttt
    16381 ggtaacgacg aaagcgccta aagccaaata tcagaagaaa cgagataatg cagatgaaag
    16441 ccagtttcag atgttaaaac gattggaact gccctattcg aaattagata tccttcaaaa
    16501 ggagtgcgaa agaaaccgag ttctatttct ctcaacggct tttgacctcg agagccttga
    16561 tgcgctacat aaaatgaaat tgcctttttt taagatacct tctggtgaag tcactaatta
    16621 tttatatctc aaaaaaatcg ctcagtttgg acgtcctgtt attttatcaa caggtatgtg
    16681 tacattagac gaagttgaaa aagcaatacg tcttttagaa aaaaatggta tacgacgttc
    16741 gttcattacg ctacttcatt gtaataccga ataccccaca ccgtacaggg atgtgcacct
    16801 tagggcgatg caaacattgc gctcagagtt tggcgttaac gtaggttact cagatcatac
    16861 aatgggtacg gaggttgcta tagcagccgt tgcattggga gctacggtta tagaaaagca
    16921 ttttactttg gattgtggca tgagtggtcc tgatcataaa gccagtatag agcctcgtga
    16981 ttttaaaaga atggttgacg ccattcggca tatcgaaaca gcgttaggga gaagtacaaa
    17041 gaaaccttca gcatctgaga aaaaaaatat taaaattgcg agaaaaagta ttgttgcttc
    17101 acaagatata aaaaaaggtg attttttttc tgaaaagaac ataacagtaa aacgaccagg
    17161 aacgggtatt agtcccatgc aatggaatcg cgtgataggg cgtcaggcca aaaaagattt
    17221 taatcttgac gatttgatca gtatatgaaa aaacaaaaaa ggcacaagtt gtgtgttgtg
    17281 acaggtgcta gatcggagta tggacttttg cgtcctctga tctttgctct ttcaaagtca
    17341 aaaaattttg atttatgcgt attggtcaca ggtatgcatt tgtcagaagg atttggaatg
    17401 actgagtctg aaatagaatc cgatggtttt gaaattcatg agcgtgttga gatgctgctc
    17461 gattcgatgt cacaaattgg tgtttctaaa tcaataggtg tcggcatgat tggactaagc
    17521 gacgccttgt cgcgtttaaa tccggatgcg atgattattt taggggatcg atttgaagcg
    17581 tttgctgctg ccgctacggc gcatgttttg agaataccga tcattcattt acacggtggc
    17641 gaattgacag agggttcgat tgatgacggt tttcgccatg ctataacaaa gatgagttcc
    17701 ttacattttg tgtcaaccga ggtatataga gatcgggtga ttcaacttgg tgagcatccc
    17761 gaacgtgttt ttcatgtcgg cgcgatcggc atagaaagta tccgctcact taaacttaag
    17821 agcgaaaaag agttgtgccg ggaactcaat ttgccgacac ttggtaatag tttgatcatc
    17881 acatatcacc cgacaacgat tgcaaatggc aacgtcgaaa aagaatgcaa cgaacttttt
    17941 cacgcgcttg agaagtttaa taatatgact atgattttta cgggctcgaa cgcggatgct
    18001 gatggtcaac gaatcaataa attgatgcag cagtttgtag gtaaaaagta taagcaacga
    18061 aggttttttc aatcgctggg gcagttgcgc tacctttcat tacttcgatt tgtaaaagga
    18121 gtcgtaggta actcgtcaag cggcattatt gaagcacctg aatttggagt tggtactgtg
    18181 aatataggtt cacgtcaaga cggccgtgta aaaccgcgaa gtgttgtaga ttgtgcatgc
    18241 aaacgaagcg atattgagat ggctatcagg aaaattattt cacctgaatt ttcaaccgtt
    18301 ttggatgcac tagatcatcc ctacggcaaa gggaaaacga cggaagccat catggatgtt
    18361 ttgaagagta ctcagttctc aaaattaaac agaaaaaagt tttttgatat aaaaaaacac
    18421 tgacaatgaa agaatcaata gaccaatttt ttattgatcc aactgtaacg gtgcgtgatg
    18481 cgatgaaaaa actgaacagt actgggcgta aagtattgtt tgttagaact tctcaaggaa
    18541 cgctctttgg cagtattacg gatggtgaca ttcgtcgttg gatactcaaa ggtggtcagc
    18601 ttgaagctaa agcatccgaa atatgcaata taactcccca tgtcgtggaa tccaattacg
    18661 acatcgaata tgtcaaatca attatggtta gagaaaatat cgaatcgctt cctgtagtca
    18721 ccagcgatgg gtgtgtggaa aaattgcttt tttgggaaga agtttttgca aaagctaatg
    18781 ttcgtcgttc gggtttttct atagacatac cggttgttat catggctggc ggaaaaggta
    18841 ctcgtatgga tccgtttaca cgagttctac ctaaaccgct tattcctatt gctgataaaa
    18901 caattttaga gttcattttt gatgaatttg tgcagtttgg cgtggtacgt ttttttttaa
    18961 cactcaatta taagggtgaa cttatcgagg cttatgtaaa gtctttgaat aaatcatatc
    19021 aggtggattg tatttgggaa aaagattttt ttggtaccgc agggagcttg acgttagtgc
    19081 gaaatcatat cgaaagtgat tttattttat caaactgcga taatatcgtc aaagcggatt
    19141 atcatgaagt cttagagttt cacagacaga ataatgcttg gttgacaatt ttatcgtcta
    19201 ttcagaatca tcgcattcca tacggcgtcg tgtcatttga ggatggagga acggtaaacc
    19261 gaataattga aaaacctgaa tactcttttc acgtaaatac gggcgtgtat atcataaatc
    19321 gcaaagctct agattatgta cctgatcata cgatttttca tatgacggat ttgattgatc
    19381 ggcttttatt agacaaacga aaagtaatga catatccggt aacggagaat gattatattg
    19441 atatagggca atgggatgag tataaaaaag cagtagaaaa attaaaaata taatcatgag
    19501 ttataaagaa aaaagtattc tagttatcat acccgctcgt ggaggaagta aaggcgttcc
    19561 agcgaagaat attcggccgt tggccggaaa gcctttaatt gcttatacta ttgaggcggc
    19621 tacaaagtcc aaatatgtag atcgtgtgat tgttagtacg gattcggaaa caatagcgga
    19681 cacttgtaag atgtatggta ttagtattcc atttattcgc ccgtctgaat tatcaacgga
    19741 cgaatctccg acttggcaaa cagtcgaaca tgctgttgat tggttgagca acaatgagaa
    19801 aaaaacgtat gatgtgattt gtctcttaca acccacgtct cccttacgca atgtaaggca
    19861 tatcgatcaa tccatatcca aatttctgtc atgtaatgcg gattcactgg tttcagtatg
    19921 tcgatctcct aaaagcccct attggtcaca acatataacc ccggatggtt acctaagccc
    19981 attatttcca gattcaggaa attatattcg acgtcaggat gtgccggtga catttgacct
    20041 caatggtgcg atctatctgg taaaaacgga attttttagg cgaacacatt tgttcaaagg
    20101 cccacgaaca gttgcgtatg aaatggatta tcaaagttca attgacattg acactccact
    20161 cgactttgaa atagctgaga tcataagaaa acaattggga tcgcatgaat aaaagaatta
    20221 tagcacgatt ggacatcaaa ggtcctaacc ttgtcaaagg tatccatctt gaggggctcc
    20281 gcgttttagg agatccttca aaatttgctc attcatattt ccatgaagga gcagatgagt
    20341 taatatatca ggatgttgtt gctagtttat atgaccgtaa tagccttcat agctttatcg
    20401 ctaaggccgc agaacagata ttaattccac tcactgttgg tggcggtatc cgtactattg
    20461 aagacataag aaccattctc agattcggtg ccgataaggt ttcattaaat actggagtgc
    20521 taaaacgacc agaattaatt cgagaggcgg caaattcgtt tggctcatcg acaatcgtta
    20581 ttgcaattga agctataaga caaaaagatg gaaaatatta ctgttatatt gacaatggtc
    20641 gtgagttttc aggtcgcgaa gttgctgact gggtttgtga ggttgaggca cttggggcag
    20701 gagaaatatt gttgacatcg gttgatcgag atggtacagg tttgggtttt gatatagagt
    20761 tggttaagat ggtgtcgggt ttagttaata ttcctgttat tgcgcatggt ggatgcggaa
    20821 agatagaaga tatttctgag gtttttaaag ttggaaatgc ggatgctgtt gccgttgctt
    20881 ctgtgcttca ttatggaatg ataggtaaag aagtacgtgc cttgcacgac ataacagaag
    20941 ggaattttga atttttgaaa agtaataaaa gtttttcaaa aataaccccg ttatctatgt
    21001 tagaaataaa aaaacagttg atagacttag ggtacgatat aagaaattag gaaaatgaaa
    21061 acacaagtat ctcatcgagt ttctgtctgt atcattgatt atcatttggg gaatcttttt
    21121 tcggtgaaga gagcgtgtga gttaattggg ctccgtccga ttatttcttc agatccaaac
    21181 gtaattagaa attctgacgc gttgattttg cccggtgtcg gtgcatttgc agatgcaatg
    21241 agaaatctca aaaacatgaa aattgatgtt gctattaagg atcacattaa tgaccacaaa
    21301 ccattcttag gcatctgtct aggtttacag cttctttttt cagaaagcca tgagtttggt
    21361 atacatcaag gtttgggggt aattcaaggg aaagtagtta ggtttcaaaa cgaaaaccga
    21421 aaattaaaag taccgcaagt aggatggaat tttatcaaaa aaactggagg tttacctgat
    21481 gttgtttggg gaaaaactcc attatttgat attgatatta agcagcgcat gtactttgtg
    21541 cattcatatt atgtggttcc cgaagacaaa tcagttcaac tgacagaaac agaatacgaa
    21601 ggggttaaat attgttcctc ggtttttact aaaaatattt tcgcgtgtca atttcatcca
    21661 gaaaaaagca gttatgaagg attaaaaatt tataaaaatt gggcagattt tcttgagcat
    21721 aaggattaaa agatgagaaa caaaaattta gaagcgtatt acggtttgcc aaataatgtg
    21781 tttttttgca aacgatgtgt tatgtcgaat cagcgaccga cttctacaat tgaatttaga
    21841 cacattgcgg gcagcaaaaa aaacactatg aattttgatg ataaaggaat ttgtgacgca
    21901 tgtcgtcatg cagagatcaa aaaaacaata gattggaaaa aacgagagag cgaattattg
    21961 cgtttactgg aaaagtatcg gcggtccgat ggtaactatg actgccttgt tcctggaagt
    22021 ggcggcaaag atagtgctta tcaggcgcat atattgaaaa caaaatacgg gatgaatccg
    22081 ttaactgtaa catggtcacc tatcatgtac acagactatg gatatcaaaa ttggaaaaat
    22141 tggattgagg ttggagggtt tgataacctt ttatttaagc aaaacggtaa agcgatgaaa
    22201 cttctcacga agttgagcat tgtgaatttg ttacacccat ttcagacttt tattttggga
    22261 caaaaaaatt tagctccaaa ggtagctgct aaatttggta tatcgttgat attttatggt
    22321 gaaaatgaag ccgaatatgg aaatcctata gctgataatg tttcttcgtt aagagataag
    22381 tcatattaca gtttttcaaa cctcaacgag gtttttttgg gaggagtgtg tgttcctgat
    22441 ttgatttcca attatgggtt gacgttgaat gaaatcatgc cttttttacc cgcaacctcc
    22501 gatgaattaa aacgttgtga agttgaggtg cattacttgg ggtattatct aaaatggaca
    22561 ccgcaagaag tgtattatta tgctgtagag aacacgaatt ttactgcacg tccgttcagg
    22621 actcaaggaa catatagtaa atacaatagt attgatgata aaatcgatga ccttcattat
    22681 tatactacat acattaaatt cggcattgga cgcgcaactt atgatgcctc tcaagaaata
    22741 agaaacgatc acataacgag ggaagagggg caggcactgg ttcgaaagtt tgatggtgaa
    22801 tttccagatc gttattttga agaaatcatg aggtatttag atattaatcc ttcagacttt
    22861 catatgttat gtgataaatt tcgttcaccc cacttatggg cacaagtaaa tggggaatgg
    22921 aaattaagac ataccgtaaa tagtgatgga gtggatgatt aaatgcgcag ttcataaaat
    22981 ttattatatg taaatgtttt gtgagtttgt atcaaaaaaa aatcaattgg cggtctttaa
    23041 ttattaatac gatacgactt gatatctatg atctgaatgc gatcaatagc aatcaagcta
    23101 tgagatatta tgacctttta ccgcaatccc agttgcctcg taaaggcatt gttgcccgta
    23161 tggcttcatt ttatgtaaat atgtacaagt ctttcgtaac ctgtttgcag acacagagat
    23221 tagatgactt attagaatgc gaacagatgg cgtttgtaac cacgcgaaac caaaaggaag
    23281 caattcgaag cgtaatatct ttttccggcg acacgcactg ttatgggata catggacacg
    23341 gtgatataag ttttgattta ttcaaagcgt acttgcttgc tatacctttc tttccgttgg
    23401 taatgtatca
//
LOCUS       DAOQNQ010000020        22290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_791_length_22290_cov_5.407781, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000020 DAOQNQ010000000
VERSION     DAOQNQ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 22290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22290
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_791_length_22290_cov_5.407781"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..2115)
                     /locus_tag="PK754_02640"
     CDS             complement(157..2115)
                     /locus_tag="PK754_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014243884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNB08432.1"
                     /translation="MSRLIDNQASIFIRQSKGWSYLAMCGGHEGIQLALGLSFRANKD
                     FLFPYYRDQLTCLAAGISPYEIMLNGLSRKDDVASGGRHMSNHFAKPEIQIENVSSCV
                     SNHTLHAVGVARAIKYYKSDAIAFASFGDSSSSEGYVYEAMNGASREQLPVIFIIQNN
                     HYGISVPTEEQTANYVVSENFRGLKNLKIFNCDGKDFFDSRRAMDEAIAYVRSGEGPA
                     IVHADCVRMNSHSNSDRHELYRSPEELADMQKLDPLHKFRSFLVEERIFSEDEITAIE
                     NDNKNIVDEAAAKAEAAPTPDPATAMDFVTPEPIQVEETPTPDGEPTEKILQAMNRVF
                     HEEFRHNPDTFLWGQDVASKDKGGVFNATKGMQQEFGRGRVFNAPIAEDFIVGTANGF
                     SRWHDNVRVVIEAAQFADYVWPAMEQIVETSHEYYRSNGQNCPNIVIRLASGGYIGGG
                     LYHSQTVEGIMANLPGVRVVVPSFADDAAGLLRTAIRSRGITFFLEPKFLYNQIFAQS
                     PKTDENHFVPFGKARVRREGQDVTIVTYGTPVHWSLRAAGKMADKGIHAEVIDIRSIV
                     PLDMETIKRSVQKTNRVLVVTENQATGGFSGEIAARITQECFEYLDAPVTRVCSADCP
                     IPFSRILEAAVLVDEAKIAHAIENIMAY"
     gene            complement(2359..3039)
                     /locus_tag="PK754_02645"
     CDS             complement(2359..3039)
                     /locus_tag="PK754_02645"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNB08433.1"
                     /translation="MNAFLRIVWTIFEKDFLTEKRTKESFSAMLVFGILVLVVFNFTV
                     SPGSQEIQEIGAGMLWVAFTFAGTLGLNRAFASENDNGNMQALIMVPVDKGAIYLGKL
                     LSGAMFMFTVELFIIPVFVLFFNVNIWEQLPGLLLISILGTLGFIGTGTVFSAVALNT
                     RMREVLLPVLLFPVTIPMIIAAVEATAVLLRGEPLSGAFDWIKILLGFDVTIIVTSFL
                     TFEYILEE"
     gene            complement(3147..3221)
                     /locus_tag="PK754_02650"
     tRNA            complement(3147..3221)
                     /locus_tag="PK754_02650"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3184..3186),aa:Pro,seq:cgg)
     gene            complement(3302..3547)
                     /gene="purS"
                     /locus_tag="PK754_02655"
     CDS             complement(3302..3547)
                     /gene="purS"
                     /locus_tag="PK754_02655"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNB08434.1"
                     /translation="MLCTIKIKVTPKAGVLDPQGKVVMSSLETLGFNGIDDVRLGRYI
                     EIKMEASSKEEAARQADTMCQKLLANLVIEQYQVEVS"
     gene            complement(3569..5290)
                     /locus_tag="PK754_02660"
     CDS             complement(3569..5290)
                     /locus_tag="PK754_02660"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08435.1"
                     /translation="MTAQTPRFQYRLDIPRPSSDQGYVTLTLTGIKLKSATFCMPAWS
                     PGNYRIVDYGRWVDSVQAWDIKGKEISVSRLNNNEWFVKNARQLARITYRIHDIPEDS
                     VPVLPTTLNSMDRSTFFYNGPSVFGYIKGYQTEACQVEFQLPCGWRVWTPLSRISDFR
                     YAARSYDELVDAPALLGTWPIEAFRFTLDSTDYHFVINSETDIKTDTLLNLAKTIIRY
                     QNRLFDDRPSEAYYFLYNFFAAGSRYGALESTRASSYYLPPFASQRNLRSSYLIRVLA
                     HEFFHRWNPERFHPKELHIINYQAPLRIQNLWFIEGVTEYYAQLTLVRTGLLSKKVFY
                     NTFRDIAAENPSANLEALSRNASEIGVAPAMYTQGSLTAWLMDIEMRDRTDNAVSMDS
                     LMVRMHRAFGKKDKPYDDKTLITTIKQFTGVDFTSFYSHYIAGGKPLPVREILEKAGL
                     AYDIIYTPAHGWFWDVSAGGKLVVQSVSENSTAFSLGMQSGDIVTSIQEQTLPTTMDG
                     IRTRIEALESLSVGESVRLMVQRNGKPIALKGTIVASQKPTVSIKEIAAPTEKQLRIR
                     QSILSSP"
     gene            complement(5341..6810)
                     /locus_tag="PK754_02665"
     CDS             complement(5341..6810)
                     /locus_tag="PK754_02665"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNB08436.1"
                     /translation="MKKSKIFFVAPAVYPFARDGRVAEFAAGLPAQLQSMGHEVRVLM
                     PKYSLINERKHIIRDIIRMKDIPVKMYNGDLPVSVKSGFIPETKTQIYFAEQEKYYKR
                     SDLYRDKRTGEYYKDNDERFILLARTAIETLKTLGWQPDIVHCIDWQSGLLPAMIREE
                     QKQNNFFKKTIITFSVINTEEPALFDKSVFDKAFGQAHIIEKEKCFHKSKFSFLKTAI
                     MHSDAVTIPMTHKHLKTITKPANDVEAILASAKMVQESYFGGEHNYWSPVNNNTLYKS
                     YSAEKLERKVTNREGFLDDKRTGFDPAHPIFGILVEDLASQNKALSQFLKAVKDVKLQ
                     LFILANDSISSVSSLKPLITKNPNHTFFTMQDPEEQFRHNFFAVADMLYIPPSDDFAE
                     IYFMNGIRYGGIPVIREECIISDRFTPTKEKSTSGNAIIYSDEKDMVKKIAAAVSYYE
                     TDKNGWDHLVRHAMKSDANWALSASSMIKLYEKALAKLK"
     gene            complement(6828..7826)
                     /gene="galT"
                     /locus_tag="PK754_02670"
     CDS             complement(6828..7826)
                     /gene="galT"
                     /locus_tag="PK754_02670"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNB08437.1"
                     /translation="MSELRKDPIVNRWVIISEERGKRPSDFAETPSQLTTSCPFCAGN
                     EHLTPSEIMAFRHEGTMKDQPGWSLRVVPNKFPALMVEGQESHRHNGFYEFMDGLGAH
                     EVIIESPQHLCTLEEMSAPDFTNVIRAYRDRIRDLRNDTRLKYALIFKNQGAPAGATV
                     EHTHSQLIATPVVPKTVEEELRGSQDFHQTHNQCVFCGILNDDRKDNQRIVLDRDGFI
                     TLEPFAARFPFETWILPATHEVRFEDMNDEKINALASVFQETLMRLRKALNHPPYNFV
                     LHTAPLNTYVPDHAYHWHIEIIPKLTRVAGFEWGSGFFINPTAPENAATYLRQVRL"
     gene            8076..8750
                     /locus_tag="PK754_02675"
     CDS             8076..8750
                     /locus_tag="PK754_02675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNB08438.1"
                     /translation="MEGIQTTVKFAGPGDSVAVIKVGGYIDTTTSSELERTLGSLLKK
                     NQHRIVVDLGDVDYISSAGWGIFISEIKGIKENSGDLKLVRMIPEVYEVFELLEFHYI
                     LKAYDTLDEAVDAFVKESGGSMSAKPKDSTQTIQAASVAKPVTAAPVTKQASKPADEA
                     EDEEDNTPATTQGDETPDEYLARAVRQYPTLSAWQLKKVLMTSDFGFTNMGWFEIRKK
                     MKSVRR"
     gene            8903..9535
                     /locus_tag="PK754_02680"
     CDS             8903..9535
                     /locus_tag="PK754_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08439.1"
                     /translation="MDVDLVPATGMLTGRQKIIYTNNSPDTLHALHMNLYVNAFRKNS
                     RMHRYHEERDKYYGGSLISYVPEAYLGYSKVQNIRDEPGRLLSQREDDTLLEITLAEP
                     IQPGGVCTLTLDFEVKIPAQIRRMGRFNREGVSFSIAQWYPKLCVYDHRGWHKNYYLA
                     REFFGEFATWDVSITLPAHYIVGASGHLTNAENIHAMMMSEPESTSMDRV"
     gene            9581..11920
                     /locus_tag="PK754_02685"
     CDS             9581..11920
                     /locus_tag="PK754_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNB08440.1"
                     /translation="MQRKLAEITRQQSVTMPNRTWRYHAEEIHDFAFAADPDYKVEIF
                     DHRGMRVNLLCLPDVEQRWAPMKEWVTKIIDYMEAHVGRYPYPELTVAQAGDGGMEYP
                     NIVFITGRRGNFSLASVTAHELIHNWFYGLLANDETHEAWFDEGITSYYTTRLMEHLF
                     GRYANREYETEFQKKWFPREDARIATFTGWAHWVKQGYEEPVLTHSDYFKSDQSYSMA
                     TYYKGQIFMNALEYRYGRERLDSLMRDFFEVYQKHHTLTDDFKRFLEYKTGEKLDSLF
                     DQWLNTTTYCDYGIAHINNSKNTAGQNQVKVKLVRHGAISMPVDLYLKLTDGSWSGVR
                     IPARHDDPNKSGLKRWTEWPASDPTYTLTMITDLPVDRAEIDTSGRLPDIFRMNNRSG
                     FLPKTEWHWQRPLGILPTSDTYVIEHRPSLWYNQTDAARIGWKMRGKWATDEHQIKAG
                     VYAGTRSRTIDYEITYSTPMYSIGRQVFVDMASYKLDGVFQNELSFRKKWFGGAYERP
                     PYYDVSLGLRTSRRFNSAYLNPGVFWEKGDVHVLTASLQWQPQIFDSPRLKMTVETST
                     FASDYTFTKADLTYYRPWVLKRNVATLESRIFAGFADGNVPVQDQFYLAGASPRQMFF
                     NPWYRSRGTLPEKGWRDASGQRHIYYDGQGSMAGYADSNALGMHIIAINLDLKPANPL
                     KPIIKKEILFITQFEPILFFDAGGVSGKSGKRRHSIPSLVFDAGCGFRYLPAFIPSWM
                     GNYAIKAEFPLWVSDPSLAGQSGKSWAFRWLIGFENTIR"
     gene            12271..12543
                     /locus_tag="PK754_02690"
     CDS             12271..12543
                     /locus_tag="PK754_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08441.1"
                     /translation="MLKSKALLVAGLFALLTMASCKSYATREELQQLDDNKAKTKSME
                     NKVVELTNEKKRVEQEVQTKKQKLEQAKKEKSEVLKRLEEIEKNNK"
     gene            12569..13441
                     /locus_tag="PK754_02695"
     CDS             12569..13441
                     /locus_tag="PK754_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNB08442.1"
                     /translation="MKIKSLLALGLSILITGATVQAQDEVKMEKADWAAQVAAQQQQQ
                     AAQQRTIDSLNKAINGVKEEGTKVDQETEQVWQEIYAALGTDKAGVEAYKKQIADLEA
                     KVAEQSKLSDEALSKPEKQKELEALKAETMALKKDKRYALTETYNKVNDLNSRVDQVI
                     SRGKNYTPPKPRHDSYSVVMGDYLWRISGKKEVYSDPFQWVKIYSANREQIKNPDLIY
                     ANQTFIIPRSEEANEYWVQRGDNLKSIAQSRLGNPSDWWKIYNANKNVIDNINKGADT
                     KVIYPHTILIIPKN"
     gene            13532..14563
                     /locus_tag="PK754_02700"
     CDS             13532..14563
                     /locus_tag="PK754_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNB08443.1"
                     /translation="MPHTEERSIKIKGISPLVLLGPNDINLKILERAYTARIVVRGEN
                     ITLRGDASEVHQLEEIFTELIFLANKKSRLDSVDVETVIRLVKTGRDEHSSESDGVIT
                     HKNLNAVVLFTHKGFIEAKSDIQKSYIREARQNDIIFAIGPAGTGKTYLAVAIAVAAL
                     KNKEVDRIVLSRPAVETGESLGFLPGDMHEKIDPYLRPLYDALHDMIPSNKLQDYMAH
                     HVIEIVPLAYMRGRTLSNAFVILDEAQNTTPTQMKMFLTRLGANARAIVTGDISQSDL
                     PRGMMSGLREVKEILTGIEGISFVYFDKNDVVRHKLVRDIINAYDAYDDEQTGAIHRK
                     NLSIKSSNE"
     gene            14639..15814
                     /locus_tag="PK754_02705"
     CDS             14639..15814
                     /locus_tag="PK754_02705"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNB08444.1"
                     /translation="MKEVVIVSAVRTPIGSFQGALSEIVATKLGAIAVAEAVKRAGIQ
                     PTDVDEVIMGNVLTAGEGQAPARQAALFAGLPESVTCMTINKVCGSGLKAVMLATQAI
                     QCGDADVVVAGGMESMSNIPYILEKARTGYRMGHGQLVDAMIKDGLWDVYNNFHMGSA
                     AELCAKECNIPRDAQDAFAKTSYERAQKSIKEGVFKDEIVGVTVTSKKGDVVIADDEE
                     PGKANFEKMLTLKPAFQKDGTITAANASKINDGAAALVVMSREKADKLGLKPLVRIVA
                     QASSARKPEWFTTAPIDAIGKVLQKANQKIEDIDLFEINEAFAVVSMAAANQLNIPSE
                     KLNVHGGAIALGHPIGASGARILTTLIHAMNRHNKKRGMAAICIGGGEASALIVERI"
     gene            15936..17321
                     /locus_tag="PK754_02710"
     CDS             15936..17321
                     /locus_tag="PK754_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB08445.1"
                     /translation="MSVLGTAENPLRVAVIGSGPSGFYAADHLLKQKDVIVKIDMYDR
                     LPTPYGLVRGGVAPDHQKIKSVTKVYEKTAAHPMFSFYGNVELGRDVTRQELKTRYHA
                     LIYAVGAQSDRRMGIPGEDLNGSHPATDFVAWYNGHPDYRDLSFDLSQERVAIIGNGN
                     VAVDVARILASSIEELNRTDIADHALVALKKSNVKEIYMIGRRGPAQAAFTNAEIKEL
                     GELEECAITIPGTEGALDEVSKKNLEAHPDSGVSKNLEIMKDYLDKTTDGKRKKMVLR
                     FLWSPVELKGREGVEEIVLQKNILVQDADGSIKAKGTEAKETIPVGLVFRSIGYKGVA
                     LKDVPFDEKKGIIPNAHGRVTELNGTVIPGEYTAGWIKRGPSGVIGTNKPDALESVNM
                     LLEDVKSGHIHPIDQAASTTDWLQSKKIRFVTFADWLKIDKAETEKGMASGRPRIKFT
                     RIEDMLHIAGV"
     gene            17363..18967
                     /locus_tag="PK754_02715"
     CDS             17363..18967
                     /locus_tag="PK754_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB08446.1"
                     /translation="MSCVPAQQEERITVAVRTEPDAINPVTHTSAFSRIVCTGILPRL
                     LESEWDSLQGRAVYKPFLAKSYSFSDDRRVLTYHLRSDFFWEDGERVTAHDIRFTFQM
                     IAHPDVASTKKEHIQYLTDALGSVDRSVIALNDSTIEFHFTRAYPLQIFDTNLQPGFL
                     PFHILKDVKPSELRRHTINTMPLSGSYYDITIWKKQDYIVLERNARSPYQAASRQIVF
                     RIIPEYTTRLTALKTGDVDVMYPIMPQDVADLRKTNPELRIEAMRSRYYDYVGWQNID
                     QHAYHASSGNTIQPHPLFGSKHVRRALTMAIHRQEIVDGFMGEFGRVAITPISPVFRW
                     AANDTLSPLPYEPEESKRLLAQEGWIDRDGDGIVEKNGRAFTFELYYDAGNDRRQFAA
                     LIVQRDLKKIGIRCDVRTAETNVFYDDELNKKYDAFISGIGVPLMIDPTSEWSSDLKK
                     NPYNDISYQNPNVDALIEAGKRVNNPEDAAPMWRALQAILHEDQPATFLYWRDELVGY
                     NQKIKNSRTSLLGEIDKFWLWEKVTN"
     gene            complement(18964..19239)
                     /locus_tag="PK754_02720"
     CDS             complement(18964..19239)
                     /locus_tag="PK754_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08447.1"
                     /translation="MIVPIFRVLSILCGLFFLMTGWIWQYLMALFVLPVGIAGMILWF
                     SANRMAKKLPDENETTARWLNQAAWWINITAFLSSGVALLYNLVAVR"
     gene            complement(19236..21056)
                     /locus_tag="PK754_02725"
     CDS             complement(19236..21056)
                     /locus_tag="PK754_02725"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNB08448.1"
                     /translation="MKSFTELLNEKIIVFDGATGTNLQTQNLTADDFGGEAYNGCNEY
                     LLISRPSAMEKVHVDFLEAGADVIETDTFGSSSIVLAEYDLQDRAYELSKLGAALAKK
                     CALDFSTPDKPRFVAGSVGPTTKLPSLSHIDFETMRASFYEQLAGLMDGGADLFCIET
                     CQDILQTKIALCAAQDLFRERKRKLPVIVSVTVETTGTMLMGTEISAALTTLEPYDIV
                     DVVGMNCATGPQEMSDNIRFLCHNSPKHVFCMPNAGIPENIGGKAHYHLSPDDLVKWL
                     MHFSKDFGVNVVGGCCGTTPAHIKPLAEAAARLNPLKRTWNYTPSVASIYMSSPMHVE
                     PAPLLIGERTNANGSKKFRELLIAENYDEMVSMGKEQTREGAHVLDVCVAYVGRNEVR
                     DMEETIRRFNTQISIPIMIDSTEVPVIERALQMLGGRAIVNSINLEDGEERTDKVLSL
                     CKRYGAAVVALTIDEDGMAKTRERKLAIARRIRTIAVEKYGMRDEDLIFDTLTFTLGS
                     GDEEFRKAGIETIEAIRLIKQTYPGCKTLLGVSNISFGLSPQARHVINSVFLHYAIEA
                     GLDMAIVNAQKIMPLYKIDPHERELARQLIFDERKWEPAA"
     gene            complement(21139..>22290)
                     /gene="ahcY"
                     /locus_tag="PK754_02730"
     CDS             complement(21139..>22290)
                     /gene="ahcY"
                     /locus_tag="PK754_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNB08449.1"
                     /translation="GCLHMTIQTAVLIETLVELGADVTWSSCNIFSTQDHAAAAIAAA
                     GIPVYAWKGMNAEEFDWCIEQTLFFGKDRQPLNMILDDGGDLTNMVLDKYPSLAKGVR
                     GISEETTTGVHRLYERVKKGTLPLPAININDSVTKSKFDNKYGCRESLVDAIRRATDL
                     MLAGKVAVVAGFGDVGKGSAESLRDAKCRVIVTEIDPICALQAAMEGYEVKKMDTAIK
                     EADIIVTATGNCRIVTDRHFRAMKDKAVLCNIGHFDTEIDMAWLNKNYGHTMDTIKPQ
                     VDKYTVDGKEIIVLAEGRLVNLGCAMGHPSFVMSNSFTNQTLAQIELWNNSAKYENKV
                     YTLPKHLDEMVARLHLEKIGVELDELTSEQAEYIGVDVKGPYKPEMYRY"
BASE COUNT         6151 a         4989 c         5437 g         5713 t
ORIGIN      
        1 aagagcaagc cgaagtctaa aagcacgaac aaaaaaacgg tatcaaagaa gaagcaaaca
       61 agcggaaaaa agaaacgcaa ataaacgatt acattcgtat aacaaaaaag gcttcggtgt
      121 ttaaccgaag ccttttttta tgtctgcgaa aaattctcaa taggccatga tgttttcgat
      181 agcatgggcg attttggctt catccaccag caccgcggct tccagaatac gtgagaaggg
      241 gatcgggcaa tccgcactgc atacgcgcgt caccggagcg tcaagatatt caaaacattc
      301 ctgagttatg cgcgctgcga tttcaccgct aaatccgccg gtagcctgat tttcggttac
      361 gactaaaacg cgattggtct tttgaaccga gcgtttgatc gtttccatat ccagcggtac
      421 gatggagcgg atgtcgatca cttcggcatg gataccttta tcggccattt ttccggcagc
      481 acgcaaagac caatgtacgg gtgttccgta ggtaacgatg gttacatcct ggccttcacg
      541 acgcacgcgg gctttgccaa acggtacaaa atgattttca tccgttttgg ggctttgcgc
      601 aaaaatctga ttgtacaaaa atttaggctc gaggaaaaaa gtaataccgc gggagcgaat
      661 ggcggtacgc aaaagtcctg ccgcatcatc ggcaaaagac ggtacgacga cacgcacgcc
      721 cggaagatta gccattatgc cttccacagt ttgtgagtgg tagaggccgc ctccgatata
      781 tccgccgcta gcgaggcgaa ttacgatatt ggggcagttt tgaccattgc tgcgatagta
      841 ttcatggctc gtttcgacga tttgctccat cgcaggccac acatagtcgg caaactgagc
      901 ggcttcgatc acgacgcgca cattatcatg ccaacgcgaa aatccgttgg ccgtgccgac
      961 gataaaatct tcggcgatcg gcgcattaaa tacgcgcccg cgtccgaatt cctgttgcat
     1021 gcctttggtc gcattaaata caccgccttt atctttggaa gcaacatctt gtccccacaa
     1081 aaacgtatcg ggattatgtc gaaattcttc gtgaaatacg cggttcatcg cctgtagtat
     1141 tttttcggta ggttcgccgt ccggtgtagg cgtttcttcg acctgaatcg gttcgggtgt
     1201 cacaaaatcc atggcggtcg cgggatcggg agtaggcgcg gcttccgctt tggcggccgc
     1261 ttcatcgacg atgtttttat tatcattttc gatcgccgta atttcgtctt cgctaaaaat
     1321 gcgctcttcg actaaaaagg aacggaattt atgcaaagga tctaactttt gcatgtcggc
     1381 taattcttcc ggcgaacggt ataattcatg acgatcgcta ttggagtgcg aattcatacg
     1441 aacacaatcg gcatgcacga tggccggacc ttcaccggaa cgcacgtatg cgatggcttc
     1501 gtccatggcg cgacggctgt caaaaaaatc tttgccgtcg caattgaaaa ttttcaagtt
     1561 tttgaggcct ctgaaatttt ccgatacgac gtagtttgct gtttgttctt cggtgggaac
     1621 cgaaatgccg tagtgattgt tttgaataat aaatatcacc ggtaactgtt cacgcgaagc
     1681 gccgttcatg gcttcataca cgtagccttc cgaggacgaa gagtcgccga agctggcaaa
     1741 agcgatcgcg tcggatttat aatacttgat cgcacgcgcg acaccgaccg catgcagcgt
     1801 gtgattggaa acgcaggatg aaacgttttc gatctgaatt tccggtttcg caaagtggtt
     1861 ggacatatgg cgtccgccgc tggccacatc atctttgcgg ctgagaccat tgagcatgat
     1921 ttcataaggg gaaatgccgg cggcaaggca tgtcaattga tcgcggtagt agggaaaaag
     1981 aaaatctttg ttggcgcgaa aggaaaggcc gagggcgagc tgaatgcctt cgtgtccgcc
     2041 gcacatcgcg aggtaagacc aacctttgga ttggcggata aatatggaag cctgattatc
     2101 gatcaggcga ctcatatgcg ccagagtgta ccattgttta agagtgtcgc gtttgatttc
     2161 gggacgtttg gctcccgatt tggatgatgc aacagcttcc aaaatgtctc tccgtttttg
     2221 gtgaaaccta tgttttaaca actctgtttt tttcaataaa cgcggcacta tagtgaaaaa
     2281 atcaaagcga ttcaagcaaa ttgttgggtc aacgatttaa ccaaaatggt taggtgaggc
     2341 aacaaaagtc agcgtgaact attcttcaag aatgtattcg aaggtgagaa acgaagtgac
     2401 gataatggtg acatcaaaac cgaggagaat cttaatccaa tcgaaggctc cggacaaagg
     2461 ttccccgcgg agtaaaacgg ctgtggcttc gacggctgca ataatcatgg gtatggtaac
     2521 cgggaacaaa agcaccggca ggagcacctc acgcatgcgg gtattcagtg ctacggcgga
     2581 aaaaaccgta cccgtaccga taaagccaag cgtgccgagt atgctgatca ggagtaatcc
     2641 cgggagttgt tcccatatat tgacattaaa gaagagtaca aacaccggta tgataaacag
     2701 ttcaacggta aacataaaca tcgcgccgga gagcagttta cccagataaa tggcgccttt
     2761 atcaacgggc accatgataa gtgcctgcat attgccgtta tcgttttccg acgcgaaagc
     2821 gcggttaagt cccaaagtgc cggcaaatgt aaatgcgacc cacagcatgc cggcgccaat
     2881 ctcctgtatt tcctgagaac ccgggctgac agtaaaattg aataccacca acacgagaat
     2941 accaaacacc agcatggcac tgaatgattc tttagtgcgc ttttcggtaa ggaagtcctt
     3001 ctcgaagata gtccaaacga tgcggaggaa ggcgttcatg tgttcttaag ttagtatcgt
     3061 agcctaaaac aacaacacca gcttgcgctg gtgttaaaaa atttgtttta tttcgacata
     3121 aaatccatgt gttgcgatat gtatcgtcgg ggtggggaga ttcgaactcc cgacctcttg
     3181 gtcccgaacc aagcacgcta accgggctgc gctacacccc gaacataaaa caatgccctg
     3241 atacaacaag gcggggcaat catgctaaaa aaaaaactta aatgcaacaa gtttttagct
     3301 gctatgacac ttcgacctga tactgttcga tgacaagatt ggccaaaagt ttctgacaca
     3361 tcgtatcggc ctgacgtgcc gcttcttctt ttgaagaggc ttccattttt atttcgatat
     3421 accggccgag ccgaacgtca tctattccgt taaaaccgag agtttcaagc gaagacatga
     3481 caactttgcc ctgaggatcc aggactccgg ctttaggcgt aacttttatt ttgatcgtac
     3541 acagcatgtt ggatttcctg tttatttttt atggtgatga taaaatgctt tggcggatgc
     3601 gtaattgttt ttccgtcgga gcggcgattt ctttaatgct caccgtcggt ttttgggaag
     3661 cgacgatggt tcctttgagt gctataggtt ttccgttgcg ttgaaccatg aggcgtaccg
     3721 attcgcccac gctgagcgat tcgagcgcct cgatgcgagt ccgaataccg tccatcgtcg
     3781 taggtaacgt ttgttcctga atagacgtga caatatctcc ggattgcatg cctagtgaaa
     3841 aagcggttga attttcagac accgattgga caactaattt tccaccggca cttacatccc
     3901 aaaaccaacc gtgggccggt gtatagatga tatcataggc caaccccgct ttttccaaaa
     3961 tctcccgcac cggcaaaggt tttccgccgg caatgtagtg cgaataaaac gaagtaaaat
     4021 caacaccggt gaactgtttg atggtcgtga tgagcgtttt gtcatcatag ggtttgtctt
     4081 tttttccgaa ggcacgatgc atgcgcacca tcagactgtc catcgaaacc gcattatccg
     4141 ttcggtcgcg catctcaata tccataagcc atgccgtcag cgaaccctgt gtgtacatag
     4201 ccggggcaac gccgatctcc gatgcgttcc ggctgagcgc ctctagattg gccgaaggat
     4261 tttcagcggc aatatcgcga aacgtattat aaaaaacttt tttcgataaa agccctgtac
     4321 gcaccagcgt aagctgtgca taatattcag taacgccttc gataaaccaa aggttctgta
     4381 ttcgtagcgg tgcttgatag ttgatgatat gtagttcttt gggatgaaaa cgttccggat
     4441 tccaacgatg gaaaaattca tgagcgagca cgcgaatcag gtacgatgaa cgcaaattgc
     4501 gctgactcgc gaaaggcggt aagtaatacg atgacgcacg cgtgctttcc aaagcaccgt
     4561 accggctgcc cgctgcaaaa aaattatata aaaaataata cgcttcactc ggacgatcgt
     4621 caaataagcg attttggtaa cgtataatgg ttttggcaag attcaaaagc gtgtcggttt
     4681 ttatgtccgt ttcgctattg atgacaaaat gataatccgt gctatccagt gtaaaacgga
     4741 aagcttcgat aggccatgta cccaaaagcg ccggcgcatc caccagttca tcgtaagaac
     4801 gcgccgcata acggaagtcg gaaatgcgtg acagtggcgt ccatacgcgc caaccgcaag
     4861 gaagctgaaa ctcgacttga caagcttccg tttgataacc cttgatgtag ccgaatacgc
     4921 tcggaccatt ataaaaaaac gtcgaacgat ccatagaatt caatgtggtc ggtaatacag
     4981 gcacactgtc ttcagggatg tcatgaatgc ggtaggtgat gcgtgcgagt tggcgtgcgt
     5041 ttttgacaaa ccattcgtta ttattgaggc gtgaaacaga tatttctttt ccttttatgt
     5101 cccaagcttg cacactgtca acccaacgcc cgtagtccac gatacgatag tttccgggtg
     5161 accatgccgg catacaaaac gttgccgatt tgagtttgat tccggtgagc gtgagcgtga
     5221 cgtaaccttg atcggaggac ggacggggaa tatcaagccg atactgaaag cggggcgttt
     5281 gagccgtcaa tagtccggcg ctaatgaata taaaaataat tcgtatcaga acgccgcccc
     5341 ttacttcaat ttagccagcg ctttttcgta cagcttgatc atagaactcg cgctgagtgc
     5401 ccagttcgca tcggatttca tcgcatgacg aacaagatga tcccagccgt ttttgtccgt
     5461 ttcgtaatag gaaacggcgg cggctatttt tttgaccata tccttttcgt cggagtaaat
     5521 aatcgcgtta ccggatgtgc ttttttcttt ggtcggggta aaacgatcac tgatgataca
     5581 ttcttcgcga attacgggta tgccgccgta acgaatgccg ttcataaaat agatttcggc
     5641 gaagtcatcc gaaggaggta tatacaacat atcggctacg gcaaaaaaat tgtgccggaa
     5701 ctgctcttcg ggatcctgca tcgtaaaaaa agtgtggttt ggatttttgg taatcaacgg
     5761 ttttaaagat gagacggacg aaatcgaatc gttggccaaa atgaacagtt gcaacttcac
     5821 atctttgaca gctttgagaa attgagacag tgctttattc tgcgaagcta aatcctctac
     5881 cagaatacca aaaatcgggt gtgccggatc aaatcctgta cgtttatcat ctaaaaaacc
     5941 ctcgcggttg gtgacttttc gctcaagttt ttccgctgaa taggatttgt ataaggtatt
     6001 attattgacc ggactccaat aattgtgctc tccgccaaaa taagactcct gcaccatttt
     6061 ggccgatgcc aaaatggcct ccacatcgtt ggccggtttg gtgatggtct tcagatgttt
     6121 atgcgtcatc ggtatcgtga cggcatcgga atgcatgata gctgttttga gaaaactgaa
     6181 tttgcttttg tgaaagcatt tttctttttc gatgatgtgc gcctggccga aggccttgtc
     6241 aaaaacggat ttgtcaaaca aagcgggttc ttccgtgtta ataacggaaa atgtaatgat
     6301 cgtttttttg aaaaaattat tttgcttctg ctcttcgcgg atcatggcgg gcaaaaggcc
     6361 ggactgccaa tctatacagt gtacgatatc cggctgccat cccagagttt ttaaggtttc
     6421 gatagccgtg cgggccaaca aaataaaacg ttcgtcgttg tctttataat attcgcccgt
     6481 gcgtttatca cggtagagat cggagcgttt gtagtatttt tcttgctcgg caaaatatat
     6541 ctgcgttttg gtttccggaa tgaagcccga tttgacggac accggcagat cgccgttata
     6601 catttttacg ggaatgtctt tcatccgaat aatatcgcgg atgatgtgtt ttcgctcatt
     6661 gatcagactg tatttgggca taagtacgcg cacttcatgc cccatagatt gcaattgtgc
     6721 aggcaatccg gccgcaaatt cggctacgcg tccgtcacgg gcaaaagggt aaaccgccgg
     6781 tgctacaaaa aaaattttgg attttttcat atcctaaaca aactccatta aagtcgaact
     6841 tgtcgtaaat acgtcgccgc attttccggc gccgtagggt taataaaaaa accggagccc
     6901 cattcaaatc cggccacacg cgtcaactta ggaataatct cgatatgcca atggtacgcg
     6961 tgatcgggga cataggtatt gagaggcgcc gtatgcaata caaaattata aggcggatgg
     7021 ttcaacgctt tccgaaggcg catcagtgtt tcctgaaaaa ccgaagccag agcgttaatc
     7081 ttttcgtcat tcatatcttc aaaacgcact tcatgagttg ccggtaaaat ccatgtttca
     7141 aacggaaaac gggctgcaaa aggttccaaa gtgatgaaac cgtcgcggtc taatacaatg
     7201 cgttggttat ctttgcgatc atcatttaaa ataccgcaaa atacgcactg gttatgagtt
     7261 tgatgaaagt cttgcgagcc gcgcaattct tcctcgacag ttttgggtac gaccggtgtt
     7321 gcgataagct gcgaatgcgt atgctccacc gtagctccgg cgggcgcacc ttgattttta
     7381 aagatgagcg catatttcaa acgcgtatca ttgcgcaagt cgcggatacg gtcacgataa
     7441 gcgcggataa cattggtaaa atccggtgcg ctcatttctt ccagcgtaca caggtgctga
     7501 ggcgattcga tgatcacttc atgggcacct aaaccgtcca taaattcgta aaaaccatta
     7561 tgccggtggc tctcctgacc ttctaccatc agcgcaggaa atttattagg cactacacgc
     7621 agagaccaac cgggttgatc tttcatagtt ccttcgtgac gaaaagccat aatttcgctt
     7681 ggcgttaaat gttcgttacc ggcacaaaaa gggcaacttg tggttaattg cgacggcgtc
     7741 tctgcaaaat ctgacggacg ctttccgcgt tcctcagata tgatgaccca acgattgacg
     7801 atcggatctt tacgtagttc agacatagtg cgttctgaga aacatggagt atgccgtggt
     7861 atatctcaaa accgaaatcg caatgccagt cacaatatta ttattcaatc ggcgcagttt
     7921 aattgttgta atttatgaat tttttgtaaa aaagacaaat ggaaaactaa gtaaaattac
     7981 ataaacaaaa aacttgcgca tggaagcaaa ttggattaag ttgatatcaa ttgcaaaaaa
     8041 aacaaaccgt cagattctaa agaggatttc cgcacatgga gggtattcaa actacggtca
     8101 agttcgccgg tcctggcgat agcgtcgctg ttataaaggt cggagggtac attgatacga
     8161 cgacgtcatc cgaactggaa cgtacgctag gctctttgct caaaaagaac caacaccgta
     8221 ttgtggtaga tcttggcgat gtggattata tcagttccgc aggctggggt atttttattt
     8281 ctgaaatcaa gggtatcaaa gaaaacagcg gtgatcttaa gctcgttcgc atgatccccg
     8341 aagtgtatga agtttttgaa ttactcgagt ttcattatat tctcaaagcg tacgatacgc
     8401 tggacgaagc cgtggatgcc tttgtcaaag agtccggcgg atccatgtcg gctaaaccga
     8461 aagacagcac tcaaactatc caagcggcat cggttgccaa acccgtaacc gcggcgcctg
     8521 ttactaaaca ggcttcgaaa ccggccgacg aggcggaaga cgaagaagat aatacaccgg
     8581 ccacaaccca aggcgacgaa accccggatg aatatcttgc acgtgcggtg cgtcaatatc
     8641 cgaccttgag cgcttggcag ttgaaaaaag tacttatgac ctccgatttc ggttttacca
     8701 atatgggttg gtttgaaatt agaaaaaaaa tgaagtccgt tcgccggtaa atatttatat
     8761 gcatattttg tatttgaaaa gtctcccgat ccgttcgcga ggctttttta ttttagggat
     8821 agtatttttt ttcgcctcac aagccgcgcg tagtataggc cagtcctatc ttccgcgatg
     8881 gcaacaaaaa atacattatg taatggatgt tgatctcgtc ccggcgaccg gcatgcttac
     8941 cggccgacaa aaaattatct ataccaataa ttcgcccgat acactgcacg ctttgcatat
     9001 gaatctctat gttaatgcat tccgtaaaaa cagccgtatg catcgttacc acgaggagcg
     9061 ggataaatat tacggcggca gtctgatctc gtatgtaccg gaggcctatc tgggttatag
     9121 caaagtacaa aatattcggg acgaacctgg tcgcctctta tcacaacgtg aagacgatac
     9181 gttactggaa ataactttag cggagcctat tcaaccgggc ggggtttgta cgctgacgct
     9241 ggattttgag gtgaaaattc ccgcgcaaat tcggcgtatg ggtcgtttca accgggaagg
     9301 cgtgagcttt tccatagctc agtggtaccc caagttgtgc gtttatgatc atcgcggatg
     9361 gcataagaat tattatcttg ctcgtgaatt ttttggtgaa tttgccacgt gggacgtcag
     9421 catcaccttg cccgcacact acatcgtcgg cgccagcggc catctgacca atgccgagaa
     9481 catccatgct atgatgatga gcgaacccga aagtacttca atggatcgcg tttaatcatc
     9541 caacggcaac cgggattcca taccggaggg tttcgaatcc gtgcaacgca aactcgctga
     9601 aattacgcga cagcaatcgg tgacaatgcc aaaccgtaca tggcgctacc acgcagagga
     9661 aatacatgac tttgcttttg ctgctgatcc cgactataaa gtggagattt ttgatcatcg
     9721 gggcatgcgt gtcaatttat tgtgtctgcc tgatgtcgaa caacgatggg ctcctatgaa
     9781 ggaatgggta acgaagatta ttgattacat ggaagcgcat gttggacgtt atccttatcc
     9841 ggagctgacc gttgcgcagg ccggcgacgg agggatggaa tatccaaata ttgtttttat
     9901 taccggacgc cgaggaaatt ttagtcttgc cagtgtcacg gcacacgaat tgattcacaa
     9961 ttggttttat gggttgctgg ctaacgatga gacacatgag gcctggtttg atgagggcat
    10021 aacgtcttat tatacgacgc gtcttatgga gcatctattt ggccggtatg ccaataggga
    10081 atacgaaacc gaatttcaaa aaaaatggtt tccgcgcgag gatgcgcgta ttgctacgtt
    10141 tactggttgg gcgcattggg taaagcaggg ttacgaggaa cccgtgttga cgcattcgga
    10201 ctacttcaaa agcgatcagt cgtactccat ggcaacgtat tacaaaggcc agatttttat
    10261 gaatgcgctc gaatatcgtt acgggcgtga acgtcttgac agtctcatgc gtgatttttt
    10321 tgaagtatac caaaagcatc atacgctgac agacgatttt aagcgttttc tcgagtataa
    10381 aaccggtgag aaattagatt cgttatttga tcaatggctc aatacgacaa catattgcga
    10441 ttacggcata gcgcacatca ataattctaa aaacaccgcc ggtcaaaatc aggtaaaagt
    10501 caaattagtc cgtcacggcg caatctcaat gcctgtggat ttgtatctga aactgaccga
    10561 cggaagctgg agcggcgtgc gcattccggc gcgccacgat gacccgaata agtcggggtt
    10621 gaaacgatgg accgaatggc ctgcatcgga tccgacgtat acgttgacga tgataactga
    10681 tttgccggtg gaccgtgctg aaattgatac atccggacgg ctgccggata tctttcgaat
    10741 gaacaatcgt agtggtttcc tcccgaaaac agagtggcac tggcaacgtc cgctaggtat
    10801 tttaccgacg tctgatacgt acgtgatcga acatcggccg tctttgtggt acaatcagac
    10861 ggatgccgcc cgcatcggtt ggaaaatgag aggcaaatgg gccaccgatg aacaccaaat
    10921 aaaagcaggg gtgtacgcag gcacccgaag caggactatt gattatgaaa taacatatag
    10981 tacgccgatg tatagtatag gcaggcaagt ttttgttgac atggcatcat acaaacttga
    11041 cggcgtgttt cagaatgaat tatcgtttcg taagaagtgg ttcggcggcg cttacgaacg
    11101 tcctccgtat tatgacgttt cattgggtct tcgtacgtcg cgtcgcttta attcggcgta
    11161 tttgaatccg ggagtatttt gggaaaaagg ggatgtccat gttttgacgg cgtctttaca
    11221 atggcagcct caaatctttg acagtcctcg tctgaaaatg accgtcgaaa cctcgacttt
    11281 tgcgtcggat tatacattta ccaaagccga tttgacatat tacaggcctt gggtattaaa
    11341 acgaaatgtg gctacgctgg aaagtcgaat ctttgccgga tttgctgacg gaaacgtacc
    11401 tgtgcaagat cagttttatc tagccggcgc ttcgccacgc caaatgtttt tcaatccttg
    11461 gtaccgatcg agaggtacgc tgcctgaaaa aggctggcgt gatgcgtccg gtcaaaggca
    11521 tatttattac gacggtcagg gcagtatggc cggatatgcg gatagcaatg cgttgggtat
    11581 gcatatcatc gcgattaatc tggatttgaa accggctaac cctctaaagc caataataaa
    11641 aaaagaaatt ttatttatta ctcaatttga accgatactg ttttttgacg ccggcggtgt
    11701 atccggtaaa agcggaaagc gaagacattc tataccctct ctggtttttg atgcaggctg
    11761 tggatttcgc tatctgcccg cgtttattcc ttcgtggatg ggtaattatg cgattaaagc
    11821 cgaatttcct ttatgggtca gcgatccttc gctggccggg cagtccggca aatcatgggc
    11881 tttccgttgg ttgattggtt tcgaaaatac gattcgctaa accggcgctt atttaaaaaa
    11941 gaggggatat gtatggcgca acacgtaaag actccgttta aaatacttgt gataaagaca
    12001 ctgatgatca tttggcaaaa aaaaattgct atacgctatt ggtgcagagc atgcaacaaa
    12061 aagcatgatg acatcatacg atatggcgaa aaatacccta aattttcatg ttcgacctgc
    12121 ggtagcaata atatcattga cctaaagtat gaataaacaa aaattcttat tttatttgac
    12181 aaaatccgtt gaaatacgta ctatacccat agagccttaa aatctatatt attttagtac
    12241 ttataaatcc tatacgaagg agaactcact atgttgaaat cgaaggcgtt gttggtggcc
    12301 ggtctttttg cgctgctgac tatggcgagc tgcaaaagct acgcgacgcg cgaagaatta
    12361 cagcagttag atgacaacaa agccaagacc aagtccatgg agaacaaagt tgtcgagctg
    12421 acgaatgaaa agaaacgcgt cgaacaggaa gtgcagacca aaaaacaaaa attagaacag
    12481 gccaaaaaag aaaaatctga agtattgaag cgcctggaag aaattgaaaa gaataataag
    12541 taacgaagac cgaggagacc ttatcaaaat gaaaatcaaa tccttattgg cgctcggcct
    12601 ttcgatcctg atcacaggcg caaccgtaca agcgcaagat gaagtcaaga tggaaaaagc
    12661 cgattgggct gcccaagttg ctgcgcaaca acaacagcaa gccgctcaac agcgcacgat
    12721 tgacagcttg aataaagcta tcaacggtgt caaagaagaa ggcaccaaag tagatcaaga
    12781 aaccgagcaa gtttggcagg aaatctatgc cgcccttggt acggataaag ccggtgttga
    12841 agcgtacaaa aaacagatcg ctgatcttga agctaaagtt gctgagcaat caaaactgag
    12901 cgacgaagcg ttgagcaaac ctgaaaaaca aaaagaactc gaagcactca aggccgagac
    12961 catggcgctt aaaaaagaca agcggtatgc attgacggaa acatataata aagtcaatga
    13021 tctcaatagc cgtgtggatc aggtgatcag ccgcggaaaa aactatacgc cgcccaaacc
    13081 gcgtcatgat agctatagcg ttgtcatggg tgattatttg tggcgcatct ccggtaaaaa
    13141 ggaagtgtat tccgatccgt tccaatgggt aaaaatttac tccgctaacc gcgaacaaat
    13201 caaaaatccg gatttgatct atgccaatca gacgtttatc attccgcgtt ccgaagaagc
    13261 caatgaatac tgggtacaac gcggtgataa tctgaagtcg atcgctcaat cgcgtctcgg
    13321 taatccgtcc gactggtgga agatctataa cgcgaataaa aacgttattg ataacatcaa
    13381 taaaggcgct gataccaaag tgatctatcc gcacacgatc ctgatcattc ctaagaacta
    13441 atgtctggcg aatgacacgc gtgtcaaaac tgtaaacaaa aagctcccgg atgaatgcat
    13501 tcgggagctt tttctttgtt aaggagttat tttgcctcat accgaagaac gtagtatcaa
    13561 aatcaaagga atcagtccgc tggttctttt ggggcccaat gacatcaacc tgaaaatact
    13621 tgagcgtgct tatactgcgc ggatcgtagt gcgcggtgaa aatataacac tccgcggcga
    13681 tgcgagcgaa gttcaccaat tggaagagat ttttacggaa ttgatatttt tggctaataa
    13741 aaaatcacgc ctagattcgg tggatgtgga aacggtgata cgtctcgtaa aaaccggccg
    13801 ggatgagcat tcgagcgaat cggatggtgt tattacgcat aaaaatctaa atgccgtcgt
    13861 tttgtttact cataaaggtt ttatcgaggc caagtcggat atacaaaaat cgtatatccg
    13921 tgaagcgcgg caaaacgaca ttatttttgc aatcgggccc gccggtacag gcaaaacgta
    13981 tcttgccgtg gcgattgcgg tagcggcgct aaagaataaa gaagtggacc gtattgttct
    14041 atcgcgtccg gccgttgaaa cgggggaaag cctcgggttt ttacccggtg atatgcatga
    14101 aaaaatagat ccgtatttac gtccactgta cgacgcgtta cacgacatga taccatcaaa
    14161 caaacttcaa gattatatgg cgcatcatgt catcgaaatc gtaccgctgg cctatatgcg
    14221 gggacgtacg ctcagcaatg cctttgtgat tttagatgaa gcgcagaata ccacgccgac
    14281 acaaatgaaa atgtttctca cacgacttgg cgccaatgcc cgtgccattg tgaccggaga
    14341 catttcacag agcgatctgc cgcgcggtat gatgtcggga ttgcgcgaag tgaaagaaat
    14401 tctaacaggt atcgaaggta tttcgttcgt ttattttgac aaaaatgacg tcgtgcgtca
    14461 caaacttgta cgtgatataa taaatgcgta tgatgcttat gatgatgaac agaccggcgc
    14521 gatacatcga aaaaatttgt caatcaaatc gagtaacgaa tagagaacat agaccgcttg
    14581 gaaaagcgta ctagtttgtt aaatcaatat attatataaa gcaatcagga gtgtatatat
    14641 gaaagaagta gtcatagtca gcgcggtgcg taccccgatc ggttcgtttc aaggggcgct
    14701 gagtgaaatc gtcgctacca aattaggcgc gatagccgta gccgaggcgg ttaagcgtgc
    14761 gggtatccaa ccgaccgacg tggatgaagt catcatgggc aatgtactca ccgccggtga
    14821 aggacaggct ccggcgcgtc aagccgcgct gtttgccggt ctgcctgaaa gcgtaacgtg
    14881 tatgacgatc aacaaagtgt gcggttcggg tcttaaggcc gtgatgcttg ccacacaagc
    14941 tatacaatgc ggcgatgcgg atgtcgtggt ggccggtgga atggaaagca tgagtaacat
    15001 tccgtacata ttggaaaaag cccgcacagg ctatcgtatg ggacatggcc agctcgtgga
    15061 cgccatgatc aaagacggtt tatgggacgt gtataataat tttcatatgg gtagtgcggc
    15121 cgagttatgt gcaaaagaat gcaatattcc gcgggacgcg caggatgctt ttgctaaaac
    15181 aagttatgaa cgcgcacaaa aatccatcaa agaaggtgta tttaaagatg aaatcgtagg
    15241 cgtcaccgtg acctccaaaa aaggcgacgt tgtgattgcc gatgacgaag agcccggaaa
    15301 agcaaatttt gaaaaaatgc tgacactcaa acctgcattt caaaaagacg gcacgatcac
    15361 ggcagccaat gcctccaaga tcaatgacgg tgctgcggca ttggtcgtga tgtcccgcga
    15421 aaaagcggat aaactcggac tcaaaccgtt ggtacgtatc gtggcgcaag cttcatcggc
    15481 ccgtaaaccc gaatggttta cgaccgcgcc gatcgatgcc atcggtaagg ttttgcaaaa
    15541 agccaatcag aaaatcgaag atattgatct ctttgagatc aatgaagcgt ttgccgtggt
    15601 aagtatggct gccgcgaatc aactcaatat tccttcggaa aagctcaatg tccatggcgg
    15661 cgctattgcg ttaggccacc ccatcggcgc cagcggtgcg cgtatcttga caacgctaat
    15721 ccacgcgatg aatcgtcaca acaagaagcg cggtatggcg gcgatatgca tcggcggcgg
    15781 cgaagccagt gcgttgatcg tagagcgaat ctgatttgaa taacctttgt tcttttatgc
    15841 aagacacaga atagcggtat gacgaatctc atacattctg tgtctttttt atttttatat
    15901 tgcgccgctg tttttatcaa cttacgaggt attttatgtc cgtattaggt actgcagaaa
    15961 atccgcttcg tgtggcggtt atcggttccg gtccatccgg tttttatgcg gcggatcatc
    16021 ttttaaaaca aaaagacgta atcgtaaaaa ttgatatgta cgaccggctt cccacgccct
    16081 atgggcttgt tcgcggtggc gtggcgccgg accatcaaaa aatcaaatcg gtcaccaaag
    16141 tgtatgaaaa aacggcggcg catccgatgt tttcttttta tggaaatgtg gagttgggac
    16201 gcgatgtaac gcgtcaggaa ttgaagacgc gttatcatgc tttgatttat gccgtaggtg
    16261 cacaaagtga tcgccgcatg ggcattcccg gggaagactt gaacggcagc catccggcta
    16321 cagactttgt agcctggtat aatggccatc cggattatcg tgatctgtca tttgatcttt
    16381 ctcaagagcg cgttgctatt atcggaaacg gaaatgtcgc ggtggatgtg gcgcgtattt
    16441 tagccagcag tattgaagaa ctgaacagaa ccgatatcgc ggatcatgca ctggtcgcct
    16501 tgaaaaaaag caatgtaaaa gaaatataca tgatcggtcg ccgcggtccg gcgcaagccg
    16561 catttacaaa tgcggagatc aaagagctgg gggaattgga agaatgtgcg atcactattc
    16621 caggtacgga gggcgctttg gacgaagtat ccaaaaaaaa cttggaggcg catccggatt
    16681 ccggtgtttc caaaaatctt gaaattatga aggattatct ggataaaacg accgacggta
    16741 aaaggaaaaa aatggtgctt cggtttttat ggtcacctgt cgaacttaag ggtcgggaag
    16801 gtgtcgaaga gatcgtttta caaaaaaaca tcttagtgca agatgccgac ggcagcatca
    16861 aagcgaaggg aactgaagct aaagaaacga ttcccgtcgg tttggtattt cgttcgatcg
    16921 gttataaagg cgttgcgctc aaagatgttc cttttgacga aaaaaaaggt atcataccga
    16981 atgcacatgg ccgggtaacc gaattaaatg gtaccgtcat tccgggtgaa tatacggcgg
    17041 gctggatcaa acgcggccca tccggagtga tcggtactaa taagccggac gcgctggaat
    17101 cggtgaatat gcttttggag gatgtgaagt ccggtcatat tcatcccatt gatcaagctg
    17161 catctaccac agactggctt caatcgaaga aaattcgttt tgtaacattt gccgattggt
    17221 taaaaatcga taaagccgaa acagaaaagg gcatggcctc cggaagaccg cgcatcaaat
    17281 ttacacgcat tgaagatatg cttcatattg ccggtgtatg atgattaaag ccatagcggt
    17341 ttgtttcata tcgagctttc tcatgagttg tgttccagcg cagcaagagg agcgtataac
    17401 tgtcgctgtg cgcaccgagc cggatgcaat caatcccgtg acacacactt cggctttttc
    17461 cagaattgtt tgtaccggta ttttgccgcg tttgttggag tcggaatggg attcactgca
    17521 aggtcgtgcc gtgtacaaac cgtttctcgc taaatcgtat tctttctccg acgatcgccg
    17581 cgttttgact taccacttac gatccgattt tttttgggag gacggcgaac gtgtgacggc
    17641 gcatgacatt cgctttacgt ttcaaatgat cgcccatccg gatgtagcca gcacaaaaaa
    17701 agaacatata cagtatctta cggatgccct gggatccgtg gaccgatcgg tcatcgcgct
    17761 caatgatagt acgatcgaat tccattttac tcgtgcctat ccgctacaga tttttgatac
    17821 caatcttcag cccggatttt tgccctttca cattttaaaa gatgttaaac cctccgagtt
    17881 gcggcggcac actatcaaca ccatgccgtt atccggaagt tattacgata taaccatctg
    17941 gaaaaaacaa gattatatcg tactggaacg caacgcgcgt agtccgtatc aggccgcttc
    18001 ccgtcaaatc gtgtttcgca ttattcccga atataccacg cggttgaccg ctcttaaaac
    18061 gggtgatgtg gatgtgatgt atccgattat gccgcaagac gtggcggatt tacgcaaaac
    18121 caatccggaa ctgcgtatcg aggcgatgcg ttcgcgttat tatgattatg tcggatggca
    18181 gaatatagat cagcatgcat atcatgcatc gtccggtaat actattcaac cccatccgtt
    18241 gtttggttca aaacacgtac gccgcgcatt gacgatggcg attcaccgtc aggaaatcgt
    18301 agatgggttt atgggggaat ttgggcgtgt ggcgatcacg ccgatttcac cggtctttcg
    18361 ttgggcggcc aatgatacgt tgagtccgct gccttatgaa cccgaagaat cgaagcgtct
    18421 tttagcgcaa gaaggttgga ttgatcgcga cggcgacggc atcgtagaaa aaaacggcag
    18481 agcttttaca tttgaactat actacgatgc gggcaacgac cggcgccaat ttgcggcgct
    18541 tattgtacag cgtgacctaa aaaaaatcgg catccgatgc gacgtacgaa ccgccgaaac
    18601 aaatgtgttt tatgatgacg agttgaataa aaaatacgac gcctttatct ccggtatcgg
    18661 cgtgccgctt atgattgatc cgacaagcga gtggagttcg gatttgaaaa aaaatccgta
    18721 taacgatatt tcatatcaaa atcccaacgt agatgctttg attgaggccg gcaagcgcgt
    18781 aaacaatccc gaagatgcag cgccgatgtg gcgtgctttg caggcgatcc tgcacgaaga
    18841 tcagccggcg acttttttat attggcgtga tgaattagta gggtataatc aaaaaataaa
    18901 aaattcgcgc accagtctcc tgggggaaat cgacaaattc tggttgtggg aaaaagttac
    18961 gaattaacga acggcaacca gattatacag caaagccaca ccggacgata gaaaagccgt
    19021 tatattgatc caccacgcgg cttggttcaa ccaacgtgcg gtggtttcgt tttcatccgg
    19081 taatttcttc gccatccggt tagccgaaaa ccataaaatc attccagcaa taccgaccgg
    19141 caaaacaaat aaagccatca gatattgcca tatccatccg gtcatcaaaa agaataatcc
    19201 gcaaagaata gacaatacgc gaaaaatagg gacgatcatg cggcgggctc ccatttgcgt
    19261 tcatcaaata tgagttggcg cgcaagttca cgttcatgcg gatcaatctt gtacaagggc
    19321 ataatttttt gcgcattgac gatagccata tccaggcctg cttcgatagc gtagtgaagg
    19381 aatacggaat tgatcacatg gcgcgcctgc ggcgaaagac cgaaagaaat attgctcacg
    19441 ccgagtaagg ttttacagcc ggggtaggtt tgtttgatca aacggatcgc ctcgatcgtt
    19501 tcaattccgg ctttacgaaa ttcttcatca ccggagccca gcgtaaaagt cagtgtgtcg
    19561 aaaataagat cttcatcccg catgccgtat ttttcgacgg ctatggttcg aatgcgtcgg
    19621 gctatggcta acttacgttc acgcgttttg gccataccat cttcgtctat tgtcaacgcg
    19681 acgacggccg ctccatagcg tttgcacaga ctcaaaacct tgtccgtacg ttcttcgccg
    19741 tcttcgagat tgatcgagtt gacgatggcc cgacctccga gcatttgcaa ggcgcgctca
    19801 atcacaggga cttcggtcga gtcaatcata atgggaattg aaatctgggt attgaagcgc
    19861 cgaatggttt cctccatatc acgcacttcg ttgcgaccca catacgccac gcacacatca
    19921 agcacgtgag cgccttcgcg ggtctgttct tttcccatgg aaaccatttc atcgtaattc
    19981 tcggcgatca ggagctcacg gaattttttg gagccattgg catttgtgcg ctctccgatg
    20041 agtaaaggcg cgggttcgac atgcatcggc gaactcatat aaatggaagc aacggacggc
    20101 gtataattcc atgtacgctt taatggattg aggcgtgcag ccgcttcggc gagaggtttg
    20161 atatgcgccg gtgtagtacc gcaacaacca cccacgacgt tgacaccgaa atctttagaa
    20221 aaatgcatca accatttgac taaatcatcc ggcgataggt gataatgcgc tttaccgccg
    20281 atgttttccg gtatgccggc attgggcata caaaaaacat gtttgggtga attatgacac
    20341 agaaaacgaa tattgtcgga catttcctga ggcccggttg cacaattcat acccacgaca
    20401 tcgacgatgt cataaggctc tagcgtagtc aaggccgcgc tgatttcggt tcccataagc
    20461 atcgtgcccg tagtttctac cgtaacggaa acaatgacgg gtagttttct ttttcgctca
    20521 cggaacaggt cttgtgcagc gcacagggcg atcttggttt gtaggatatc ctgacaggtt
    20581 tcaatgcaaa acagatcggc gccgccatcc attaagccgg ccaactgttc ataaaacgat
    20641 gcgcgcatgg tttcaaaatc aatatgactg agcgagggta gtttggtcgt cggccctacg
    20701 gaaccggcca cgaaacgcgg tttgtcggga gtagaaaaat ccagcgcaca ttttttagct
    20761 aacgcggcgc ccaacttgct caattcgtaa gcgcgatcct gaagatcgta ttcggccaaa
    20821 acaatggatg aggaaccaaa cgtatctgtt tcgatgacat cggcaccggc ctcaagaaaa
    20881 tccacgtgta ctttttccat agcggaaggt ctggaaatga gcagatattc attgcatccg
    20941 ttataagctt cgccgccaaa atcatcggcc gtgagattct gggtttgcag attggtgccg
    21001 gtggcgccgt caaatacgat aattttttcg ttcagcagtt cggtgaatga tttcacaagc
    21061 aacctttggg ataatataaa aaaaagcgat cctaaactct taggatcgct caatgatcat
    21121 cagagtcgtg cgtaattatt aataacgata catctcaggt ttgtacggcc ctttgacgtc
    21181 cacgccgatg tattcggcct gttcgctggt caattcgtcc agctcaacgc cgattttttc
    21241 gagatgcaaa cgtgctacca tttcgtccag atgtttgggc aaagtataca ctttgttttc
    21301 gtacttcgca ctgttattcc acaattcgat ttgcgccagc gtttgattgg taaacgaatt
    21361 agacatcaca aacgaagggt gacccatagc gcagccgagg tttaccagac gtccttcggc
    21421 aagaacgatg atctctttgc cgtccacggt atatttatca acctgcggtt tgatcgtgtc
    21481 catggtgtgg ccatagttct tattgagcca agccatatca atttccgtgt cgaagtggcc
    21541 gatattgcaa agcacggctt tatctttcat agcgcggaaa tggcgatcag tcacgatgcg
    21601 gcagttgccg gttgcggtaa cgataatatc ggcttctttg atggctgtat ccattttttt
    21661 gacttcataa ccttccatcg ccgcttgcag tgcgcagatc ggatcgattt ccgtaacgat
    21721 cacacggcac ttggcatcgc ggagggattc ggccgagcct ttgcccacat cgccaaaacc
    21781 ggcaacgaca gcgactttac cggctaacat cagatcggta gcgcggcgaa tggcatccac
    21841 aagcgattca cggcaaccgt atttgttatc aaattttgat ttggtaacgg aatcgttgat
    21901 gttgatcgcc ggaaggggaa gcgtgccttt tttgacgcgc tcataaagac gatgaacgcc
    21961 ggttgtggtt tcttcggaga taccgcgtac gcctttggcc aatgacggat atttatccag
    22021 gaccatattg gtaagatcac cgccgtcatc aaggatcata ttgagtggtt ggcgatcttt
    22081 accgaaaaac aatgtttgtt caatacacca gtcaaattct tcggcattca tacctttcca
    22141 ggcgtagaca ggaatacctg cggcggctat ggcggcggcg gcatgatctt gtgttgaaaa
    22201 aatattacag gatgaccagg tgacgtcagc gccgagttcg accaaggttt cgatcaaaac
    22261 ggcggtttga atcgtcatgt gaagacagcc
//
LOCUS       DAOQNQ010000021        22156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_796_length_22156_cov_5.267544, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000021 DAOQNQ010000000
VERSION     DAOQNQ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 22156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22156
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_796_length_22156_cov_5.267544"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..2379
                     /locus_tag="PK754_02735"
     CDS             223..2379
                     /locus_tag="PK754_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB08450.1"
                     /translation="MKNSLLCAVMIAHFFPFLLIGQNKDSTRIYNLEGTTITSTRVPE
                     SIIEVPLAVSVIDRKEFDTKRGFGMNDAMIHIPGVLAQSRSGGTDLRISIRGFGTRGA
                     GDRSNAGTSRGIRVLLNGLPLTEPDGRTALDLIDMNMVDRMEVVRSNASSVWGNAGGG
                     IVDISTLPSNSGRFAVMQAKTGSFGLREWHARTGTTIGSTQFYAGYTQNSYDGWRANS
                     QAERRILTMGLQSSVTEQTTLTALLVGGHNQFSIPGPLTKQQYDSLPTQANPAYKTQR
                     ERRNNKILYMGLGLQHNINDENQISGMAFVNPKVLHRSERNTYRDFTRYHIGGNTMYR
                     HNHEWGALKTITLVGGDLAYQDGAILFYPLTDGRRDIPAKDTSRFQDKKEGANNFGLF
                     IQNEFKFSERWTLLTGLRYDDVTYHSQNFLKAKPGLKSKSFKHWTPKAGITYLVTPTH
                     SLYANMGGGVEVPAGNETDPSNTAGQYSDTTYAFNPLLKPITSVTGEIGTKNVMVTDG
                     FLKGITYDAALFWIQVTNDVIPYRSGRFYMTAGKTQRIGLEISEKTDFAKGFSLKTSA
                     TMSKNTYEKYRIDSSLINTGSAGKFSDYSDNKQAGVPDFYYSVSGRWAPDFVKWIFAE
                     LTIQGIGSYYTDDANTIKVPAYTVLNASIGIGEPIRLSEHYTIRGFISVNNLMDKKYA
                     GSTFINPDLNSVDKKPMYLEPGLPRNFAASISLTWE"
     gene            2596..3072
                     /locus_tag="PK754_02740"
     CDS             2596..3072
                     /locus_tag="PK754_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08451.1"
                     /translation="MQLRDQDRLKDIYLNTDFDSFRNGFMRNFTYSNVSALIVWLYDY
                     VVHPNNDFFYWFRLFGVSASIYFSAWFVEMLWWKFKGANHETPFMVEAYTERIVLSSI
                     AGGIFFPLAMLLCRLTEPELIWPWLPLVGFSVALFVQIFIQWSQLQKLRKLSLTNL"
     gene            3082..4020
                     /gene="trxB"
                     /locus_tag="PK754_02745"
     CDS             3082..4020
                     /gene="trxB"
                     /locus_tag="PK754_02745"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNB08452.1"
                     /translation="MTLSAEVKNVIIIGSGPAGFTAAIYASRANLNPVIFEGHQPGGQ
                     LTITTEVENYPGFPKGVMGPEMMEMLREQAKRFGTECVFRSIDKVDFSQRPFKLTDDS
                     GEVHLAKAVIIATGASAKLLGFPKEKIMMGHGVSACATCDGFFFKGKHIHVVGGGDTA
                     MEEAGYLTKFAASVTIIHRRNTLRASKIMQERVQKNEKVKFIWDSEVVDILGDENTQI
                     QGLKLRNTVNGALSEVPSQGLFLAIGHQPNTHLFNGILDMDESGYLITQGKSTKTNIE
                     GVFACGDVQDQTYRQAITAAGSGCMAAIDAERFLGH"
     gene            4050..4787
                     /locus_tag="PK754_02750"
     CDS             4050..4787
                     /locus_tag="PK754_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08453.1"
                     /translation="MLRGLITCLTALSLSFVVSCSSGSKMYQHSDLQNKQSGLSGRSA
                     TVHIAGIDFDCKIVGLNKDVLIVQQSTLRSEIPLDAISKLEVRGSMSHKTKMLIGGGA
                     GLLVGTGAGLIVGPKVSETKGAKSLAGPLPMLMIIGGTVGGGFLGSSLAKNDEYVFNA
                     EIKPYSIHTEIGTEATPAELVAYGIFDDLSTGADEKILQVQVFQLKDNRYFIMYDESY
                     LGAYRVGGKIVNEEYIKKQAEKVSKKQ"
     gene            4892..5620
                     /locus_tag="PK754_02755"
     CDS             4892..5620
                     /locus_tag="PK754_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08454.1"
                     /translation="MQGEADSASALWTKKVWDALGGKQRFDEVRYISFDFSVISDGKE
                     VAKHRHVWDKHSNRYRVEGKNRAGEVYSILFSDLNEKKGTAWVNGQIQSGDALKKQLD
                     YGYGRYINDTYWLIMPWKWRDHGVRLRYDGTQKDSITGKALQTLHLSFAQVGLTPGDQ
                     YWVMIDPTTSRMESWKFLLQGLGAEKGHYLWQDWISVNGISFSTTKISDDEKRKILTN
                     NIRTYEVLSDSVFLFSDYTISARD"
     gene            5613..6467
                     /gene="aroE"
                     /locus_tag="PK754_02760"
     CDS             5613..6467
                     /gene="aroE"
                     /locus_tag="PK754_02760"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNB08455.1"
                     /translation="MINDWITGTTTLYGIMGYPVSHSLSPIIHNTAFHYLKIASCYVP
                     WNVFPEKFETALRGAQALGIKGLNITIPHKETALYLCDMVSDEARAIGAVNTIVFTEN
                     LIRGYNTDAYGFFRALQTCNIDQKNKNILILGAGGGARAVAYALGNFFPESTIFISNR
                     TTEKANKLVKHFNNMFEKNDLFRSVTASDMKTIQFSLVVNTTPPQSEHFDEQLFKRYT
                     GTAIDIAYSINDTPFLSMAKKHQWIISDGLEMLLFQAAQSFKLWTNEEMPMDAVRRVL
                     YSYIGRSL"
     gene            6464..7024
                     /gene="amrA"
                     /locus_tag="PK754_02765"
     CDS             6464..7024
                     /gene="amrA"
                     /locus_tag="PK754_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNB08456.1"
                     /translation="MNLDNETKKTLIHIARMTINAAVKRTKEPNYEKDVATFPILKEK
                     AGAFVTIEKRGHLRGCIGYIEAREPLYKTVIMAARSAALNDDRFGSLQLDELNEIELE
                     ISVLSTFSRVEDINDIMVGTHGLMVESGFYHGLLLPQVATEYQWDRITFIEQTCHKAG
                     LNKHAWKEKTTVIKSFTAIVFNERDI"
     gene            7043..7435
                     /locus_tag="PK754_02770"
     CDS             7043..7435
                     /locus_tag="PK754_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HNB08457.1"
                     /translation="MILILCFCCEFNAYARSGGLQVFCLAPAVYSKNRSESEISEKIQ
                     QKINYYSLCYSQNFDNKTATSFKVRFRFVIKNDGSIVDITTVFSGTKNQAFIECLTNM
                     LKQIYFQKIGTEAGDTIVEQSILFKIQS"
     gene            7524..11936
                     /locus_tag="PK754_02775"
     CDS             7524..11936
                     /locus_tag="PK754_02775"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB08458.1"
                     /translation="MKLIRIPFLSLIVIWGCSQVQLSTQNDKARHYYLEGLKLEKKNS
                     DAIHAALENYKNAIREDATFYEAYVTIASLYEKQGSYRKAALYFEKALALRHEPGRAA
                     LPYARTLYNLNRFEESYELAKYYNENYPSDAQGLSFLADAARALNKPGIESDLLRLTK
                     MDSANAYAWSSLAQYYYDQGDFAMAIPIYDRMLREKLHVTTDMRYEYALCQLHVGLWN
                     DALPHLEQVIREGINDKYPPHLAQAVRLIISGTFKEEALNLFLKAMIAFESVQNGHTD
                     RQEGYGLAKQSLTKSLEIEPNFFLSRHLAARLLYVMEEYDEAFINYEWLTKNNMADSD
                     DFANMGFLLFKKNQMKEAQAYYEKSLSIKPDQPSVKSSLETIRRINEGKISPQAYVYF
                     DKALAHTSGFDSTSYYLRLAIAADSLYPEAYLQLGLLQYRNGQYKAAEPTLLHGLQLA
                     YTDKLKKNFHYNLALVYSRLDWHDKSIQQFLQCLRIDTADGDALYYLSKVYADKSDYE
                     NAVSIYETLSRRVPEYFVPDENDLQQAGIGQFSQSIGPSRFYFDDSLHIGQTNTYRLK
                     IKSKNDALLGADANGDLSREIIITFQEQVLDITSYGVVEFALDILEINGYAIAAKEKA
                     IQGQRFYLRISDIYGVTNIYGLMEEDPAGLSRLVIAVMEDLHGNFTRRVISEGEIWRS
                     GQNIFKLGNLDAVTELDDINNQIAYGKKYYGVLGSYDAARYGETGYVTIQNKGESEFE
                     FDIQKRLIRKLKNVFVTKEFRDATAKLTQQDASYAVDLIHTTIENLEKPKKVVLENIP
                     YVRQHGPQCAAASLSMVLSYYKQSIDQDEIYEKIKSDYAGAQSNDIIHYPRSLGKYKS
                     FGYVGTLDDLKRRIDQGIPVLVFLSPFGFGHVVVVIGYDETKHQIIMHDPTVANNHAI
                     PYDDFLREWQQSGNECAIVLPLETPVVVTEEPLNSYQAVEKKWEGDKASGVRNFDKAI
                     QLYRESLRLKPNYEGAMEGMMLLHLQKDEFDKASVIMDTLLQMRPNSIDLILRRAGLL
                     LSQYDYDKVLQITRRAKQIDESNITNYLYSASALFSQKKYNEAMNELKQAIRINPLIS
                     QPRTFYASILAETGDFEKAHEQMNFALRYEPENIGNYLSVAGLYQTEINVNFLTAQEK
                     ADRIKKAIEALDIVKQANPSLPNLDQIYADIYTAVDDFKRGDSLMFENIRKYPEENGA
                     YNNIAWRYAVSGIKLDVAEKYSQKSIELSQRNPYYFDTIGWIHLKMAVQVRKIYPDSA
                     ESFMKKAESEFLATIEYDMYSDFAYRHLGVLYAVWGKLAQATTEFEKVAAMMPDKARI
                     YAEIGQDCEELGLYEISMNYYRKAIDIRPNFAYAKYRMAYILAIKMNDYTKAESYILE
                     AIQVDSVNFLYTGMQGIIAFLKQDIPVAIRILDKVCFNQSAFTDEEAAINNYYFGMAL
                     LKTNQIAQAKTQFMEYLRRSPDGEYSEIVKKRIK"
     gene            11953..12591
                     /locus_tag="PK754_02780"
     CDS             11953..12591
                     /locus_tag="PK754_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HNB08459.1"
                     /translation="MKKLGLIGLGIFAVWLSSCSVAKQIDMAKTFAKCDFSVQNIEHM
                     QLADIKIQNVKSAKDLSLKKAAQLTDALTKKTLPLTFTINLSVENPNEKPAGMNRLEW
                     IVLIDNNEIVRGALDQIVEILPDSDATVPLNIEMDLKKSFSNESAESLVKLAFNLAGE
                     GDKPTRITVKVKPTINVAGLNIEYPGYIDVHTKINSEQGKQLRKKAAKKAKK"
     gene            complement(12796..13290)
                     /locus_tag="PK754_02785"
     CDS             complement(12796..13290)
                     /locus_tag="PK754_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB08460.1"
                     /translation="MKTEKTQKPNLQQLTSTHQEIDLISNSKKPTVLLVEDDDIAGST
                     IAMMVETLHCSVIRVENGLDALSIVGSMKIDLVITDLCLPVMSGTEIIEHIRKINKTV
                     PIFIVTGYLHTATETEMISVSDKKSCGVIEILMKPIRMKNLLDKIKEHTLIENAPKAA
                     LMKI"
     gene            13553..15709
                     /locus_tag="PK754_02790"
     CDS             13553..15709
                     /locus_tag="PK754_02790"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNB08461.1"
                     /translation="MKKQIFILAGMLCLSNFSISSKLMAQSGIDSIDYWKTQAVENIR
                     EFERLPVTVKAVSLIFGDKISRAKKNIIRALVVKPEDVESKYIYAIVQRYESQFDDAE
                     KNIKSIIDVNDRFSAYGFPNVWIQLGYIYKSKMENDLAIDAFKQGALIDLNDTWPLIE
                     LSFVFSEIGKDVEACEAFYGGLNEINDPQKIEKLFVDCRDIASEKEIKIWEDSLKANK
                     EKLEFLKIFWLKRDPNPLDPVNQRLLEHYKRLIFARSNFSKSLSPWYDDRGQTYIRLG
                     KPDIRYIGRTAEAVKENESWVYDRIKSGLYFDFIELNGSYKLGSLFDALNMSSRASSM
                     VEIFNERSHINPYYQRMADRIKTQTDVVHDRAVEAFNNTVSTKPGEGSESHLNAVDRS
                     SFASTFLNTNNYSQDYLANIEYSKKQNFIFDVGAPHLPMNCNFASFRSVNKKDSRLEF
                     YYTVPFKVLNFAPSITQVDKFNTSIKLHMKLYDLKYNEVKSVERDFDISAFGKETTSH
                     FFLDQFDADITPGKYIAAVEMRNNEKDRVGIYQFVLNVRDYNSDSLLVSDIEIAQYVD
                     QTLTKDKYLKPQSNLKVVPNPAAGLVNTKPLTIYYEIYNLAKNADNRTSYQVSYSIKM
                     LESNQSFLSSVAGVFTGKKEASTSSVTVKEGKSVNEKEYIAFDIADLPTGVAALEVRV
                     KDLNSGKEAVSATNITITSAREEQNNASSEESVKEK"
     gene            15709..16566
                     /locus_tag="PK754_02795"
     CDS             15709..16566
                     /locus_tag="PK754_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08462.1"
                     /translation="MLKYKFYICCAHIIALGLAAQTYETQSSPIVIKKQNEMVVSKPS
                     TLKAIGLSILMPGAGELYLKKGKRSYWAAGLFMVAETATWITYFSYRNAGLKKEKEYI
                     RYADKNWNVDEYLRFLETTLQLSDGYLGRKSSGIDKDKLEVAENEWGSLSGISVHHLY
                     KSGRQQYYEMIYKYPEQFGQGWSDANWDLVDIPNRSTGYTPYNLTDRMKYYRSMRVKS
                     NAWLENARNATGYMLANRVLSVLDIVWITKKYKASNSTESVQMGIRIKPVKDLNLRLK
                     AYPSLEVTF"
     gene            16566..17036
                     /gene="ribH"
                     /locus_tag="PK754_02800"
     CDS             16566..17036
                     /gene="ribH"
                     /locus_tag="PK754_02800"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNB08463.1"
                     /translation="MPTSSLKSPNLSFGIIKSAFNQNVVNLLFEGLARGFQDAGIDQK
                     YIDVYDAPGAMELPVSAKHLCETGKYSAVIALGCVIRGNTPHFEYVSAETTRGLSQVS
                     LTTGIPVIFGVLTVNTLEDAMDRVKNDKSNKGYEAAMAAVEMAKLILRIKSSRQ"
     gene            17044..18750
                     /gene="recN"
                     /locus_tag="PK754_02805"
     CDS             17044..18750
                     /gene="recN"
                     /locus_tag="PK754_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNB08464.1"
                     /translation="MITSLTIKNFALIEDAEIIFGKGLNIITGETGAGKSILLGAISM
                     ILGERASTDHIRNGADKAIIEGIFHTKPNRFLKAIFTENDLGWSDELVLRREISTRGQ
                     NRCFVNDNLITTGLLKSIGDYLVDLHGQHEHQSLIRQEFHIEFLDAFANNAENVQQLT
                     ELYKSVTALQRRIHEIETNKQSIIDKKEYYSFQLKEIEEVNPQPGEDESLIQEEKLAS
                     NSEKIYELSHSAYQDLYESDGAIIERLEQVKSKLNQLSSFDERLIDYQKDFDSAVIAI
                     AEIARVLGEYNHKIVFDPERLDLMRARLMNLQKLKRKYGSIEDIIRAKEEYRGYVSLS
                     ENYDEQLLALKKELNKSIESYGNCAHAVSAARIKAAEKLDKRVVQELVKLGIEKAQFK
                     TMITQIEAPNGWFISKDKSIQATAQGIDHVEFFISANAGESLKPLVKVASGGEISRIM
                     LSLKTALAQSDKIPTMIFDEIDVGISGRIAQAVGIALSELSESHQIISITHLPQIAAL
                     AQTHFSVQKQTQGHHTTTTIRQLKENERETEIAKLLGGATITDAAIKNARDLMRQKVG
                     FL"
     gene            18790..19356
                     /locus_tag="PK754_02810"
     CDS             18790..19356
                     /locus_tag="PK754_02810"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNB08465.1"
                     /translation="MNHLIEIKKSLKRASTVEAKFATLKAVPTAKGVYGVRMPVLNNL
                     AKTYKTCDFKLINELWKSGKFEERMLSAKIIGFKAKKEPVKTLSTIRKFSASVEDWAI
                     CDCLSSQSIRSIALSEQKRIFQLSELLITSKKIWQRRMALVLLEYYTRFPQFHPYIHN
                     AIASLENEKEYYVSKAIHWLKRNMLKKR"
     gene            19383..19982
                     /gene="can"
                     /locus_tag="PK754_02815"
     CDS             19383..19982
                     /gene="can"
                     /locus_tag="PK754_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HNB08466.1"
                     /translation="MSDLKHLFQNNLHWSENIRAQKPDFFDKLSHQQAPEYLWIGCSD
                     SRVPANEIVGLLPGEMFVHRNVANVVVHTDFNCLSVIEYAVEVLKVKHIIVCGHYGCG
                     GVAAALQDSRLGLIDNWLRHIREVWKKYETELNTISDPQERHHRLCELNVVEQVANVC
                     NTTIVQKAWDRKQDLTIHGWIYGLQNGLIRDLKVSKDNV"
     gene            complement(20042..20114)
                     /locus_tag="PK754_02820"
     tRNA            complement(20042..20114)
                     /locus_tag="PK754_02820"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20079..20081),aa:Ala,seq:ggc)
     gene            complement(20166..21443)
                     /gene="dgt"
                     /locus_tag="PK754_02825"
     CDS             complement(20166..21443)
                     /gene="dgt"
                     /locus_tag="PK754_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HNB08467.1"
                     /translation="MITNTFYNEFDIQLIDGVSGRHLYERTPFQQDRDRIIYTSSFRR
                     LQSKTQVFFSGEYDFYRTRLTHSLEVAQIGRSLCHYLKEESKELKDDFYIDSDLVEAI
                     CLAHDIGHPPFGHAGETTINKLMQPYGGFEGNAQTLRMLTETIYSAASGREGMNPTRA
                     LTDGVMKYKKLYSHSHNNNQPPKNHFLYDEQWPYVQFIFDHRDIPKDVDHNKFRSIEC
                     QIMDWADDTAYSINDLLDGISSGLITQAKIESWAAKSSFTNDENVLISNLLKAMSERQ
                     IARFCSRKIGDCIRAVNLSPIKNFMSDKTNRHAFYLSINKEKRAEIDLYGRISRELVF
                     FSPQIYQLEFKGDYILRKMFDAFEKHYLGNNNRNTMTLLPDHTEGPVRNEKDLKKRAR
                     LICDYVAGMTDGFALRTFKRLYDPDFGSIMDLV"
     gene            complement(21450..>22156)
                     /locus_tag="PK754_02830"
     CDS             complement(21450..>22156)
                     /locus_tag="PK754_02830"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNB08468.1"
                     /translation="KDISSMLNDVDTAIQNALDDMGNPDVHSGKNKITFAIAQQHIQR
                     FLNNEKDRLSHDNTNRWVLSVESLMSTSIDVPIEGQLFSMRLHGKADRIDLIDDVVEI
                     IDYKTGSAQKKHVQLPTTIADLSVKSDSDKALQLLTYIILHRSHSGNKYKKYRSMIVA
                     LRGSPAEYYLPLTYDKNEIHSIETEIEKTSLEYFRSIALELLNNERPFKQTDEKKNCV
                     YCSYKNLCNRWPEHYS"
BASE COUNT         6938 a         4058 c         4647 g         6513 t
ORIGIN      
        1 atcggcagtt atgccgatga agcgtttaca ctaatttggt ctctgcttcc tataactccg
       61 tatccggaaa taccggcctt tacattccat ccgccaaatt ttagtactct acatcgggtt
      121 cagtaatcca ttttccgtgc ggtgaaatca aatataattc aaatgatcta tatttggttt
      181 gctgtacggt ttcattcatt tatttactca ggtaggagta ctatgaaaaa tagtttatta
      241 tgcgccgtta tgatagcgca tttctttcca tttttgctca ttgggcaaaa taaggacagt
      301 acgcgaattt ataatctgga aggtacgacg atcacttcca cgcgtgttcc cgaatcaatc
      361 attgaagtgc cgctggctgt cagcgttatt gaccgtaaag agtttgatac caaacgtgga
      421 tttggtatga atgatgccat gattcatatc cccggtgttt tggcgcaatc ccgttcgggt
      481 ggaaccgatt tacgcatttc gattcgcggt tttggtacac gtggtgcagg ggatcgctcc
      541 aatgcgggca cttcgcgtgg catacgtgtt ttacttaatg gattgccttt aaccgaaccg
      601 gatggacgaa cagcgttgga tctgattgat atgaatatgg ttgaccgtat ggaagttgtt
      661 cgttccaatg cgtcttcagt atggggtaat gccggaggag gtattgtgga tatttctact
      721 ttgccttcaa atagcggccg ttttgctgtt atgcaagcca aaacgggaag ttttggcctg
      781 agagagtggc atgcacgaac cggtactacg ataggcagta ctcagtttta tgcaggttat
      841 actcaaaatt cttatgacgg ttggcgtgcc aattcgcaag ccgagcgacg cattctcaca
      901 atgggcttac aatcttcagt cactgaacaa actacactta cagcgctact cgtaggaggg
      961 cataatcaat ttagtatccc agggccattg acgaaacagc agtatgattc tttgcctaca
     1021 caagccaatc ccgcttataa aacacagcgt gagcgacgaa ataataaaat attatacatg
     1081 ggtctcggtt tgcagcataa catcaatgat gagaatcaaa tatccgggat ggcatttgta
     1141 aatcccaaag tactacaccg ttctgaacgt aatacctatc gcgatttcac gcgttaccat
     1201 attggtggca atactatgta tcggcataat catgaatggg gcgctctgaa aaccataaca
     1261 cttgtcggag gagatctggc ttatcaagat ggggctattt tattttatcc gttaactgac
     1321 ggtagacgag atattcccgc taaagacaca agcagatttc aagataaaaa agagggcgct
     1381 aataattttg gattatttat tcaaaatgaa ttcaaattca gcgaacgatg gacattgctt
     1441 acagggcttc gctatgatga tgtgacgtac catagccaaa attttttaaa agccaaacct
     1501 ggacttaaat cgaaatcgtt caagcattgg accccaaaag cgggaattac ttatttggtt
     1561 actccgacac atagccttta tgccaatatg gggggcgggg ttgaggttcc ggcaggtaat
     1621 gaaacggatc cgtctaatac ggccggccaa tattcggata ccacctacgc tttcaatcca
     1681 ttactaaagc cgattacctc cgttactggg gaaataggca ctaaaaatgt aatggtaacg
     1741 gatggatttc tcaaaggtat aacttatgat gcggctttat tttggataca ggttactaac
     1801 gatgtgattc cgtatcgaag tgggcgtttt tatatgaccg cggggaaaac acaacgtatt
     1861 ggcttagaaa taagcgaaaa aacagatttc gcaaaaggtt tttcgctaaa aacgagcgct
     1921 acgatgtcta aaaacacgta tgaaaaatat cgtattgatt cgtcgctcat aaataccggc
     1981 agtgcaggga aattcagcga ttacagcgat aataagcagg ccggagtccc ggatttttat
     2041 tattcagtat cgggcagatg ggctccggat ttcgtaaaat ggatttttgc cgaattgacg
     2101 attcagggta tcggatcgta ctatacggat gatgccaaca caatcaaagt tcctgcttat
     2161 acggttctta atgcttccat aggtataggt gaaccgatac gattgagcga gcattacaca
     2221 atccgaggct tcatttccgt caataacctt atggataaga aatacgcagg ttcgacgttt
     2281 atcaatcccg atttgaatag cgttgataaa aagcctatgt atttagaacc ggggttgccg
     2341 cgtaattttg cagcatccat ttcgctgaca tgggaataat ttgatttact gagttttcaa
     2401 tgctgcccca atggttcttc gcccatagct actgtctcaa ggattgcaag caatgatgag
     2461 atttcgtcta ttgctattga tataaaatga gaaagtacag tttggtcgaa attattgggg
     2521 cagtttattt tattatttat ttgtagtcct ttttgaacat tgcgcctacc aaaaaaaatt
     2581 catactatta cctccatgca gctacgtgat caagatcgcc tcaaagatat ttatctgaac
     2641 acggatttcg attcgttccg taatggcttc atgcgaaact ttacatattc caatgtaagc
     2701 gccttgattg tttggcttta tgattacgta gtacatccca ataatgattt cttctactgg
     2761 tttcgcctat tcggagtatc ggcttcgatc tattttagcg cttggtttgt agaaatgtta
     2821 tggtggaaat tcaaaggcgc aaatcacgaa acgcctttca tggttgaggc ttatacagaa
     2881 agaatagtct taagtagcat agccggagga attttctttc ctctagctat gttgttatgc
     2941 aggctgactg aaccggaatt gatatggcca tggttaccgt tagtcggatt tagtgtcgcg
     3001 ttatttgttc aaatattcat acagtggagc caattgcaaa aacttagaaa actttcattg
     3061 accaatttat aaatatggag tataaccttg agtgcggaag ttaaaaacgt cattatcata
     3121 ggctccggtc cggcaggttt tacggcggct atttatgcgt cacgagccaa cctaaatccg
     3181 gtaatttttg aagggcatca acccggcggg caattgacca tcacaactga agttgaaaat
     3241 tatcccggtt tcccaaaagg tgttatggga cctgaaatga tggagatgct gcgtgaacag
     3301 gccaagcgtt ttggtacgga gtgtgttttt cggagcatag ataaagttga tttttctcaa
     3361 aggcctttta agcttactga cgatagcggt gaggttcatt tagcaaaagc cgtgatcatt
     3421 gctacgggag catccgccaa attgctggga tttcccaagg aaaaaataat gatggggcat
     3481 ggtgtgtcgg cttgtgcgac atgtgacgga ttctttttca aaggcaaaca catccatgtc
     3541 gtcggtggcg gcgatacagc tatggaggaa gcaggatatt tgaccaaatt tgcagcttcg
     3601 gtaacgatca ttcatcgcag aaatacgctc agagcatcca aaatcatgca ggagcgcgtt
     3661 caaaaaaatg aaaaagtaaa atttatctgg gatagcgaag ttgtggatat tttaggcgat
     3721 gaaaatactc agattcaagg ccttaaactt cggaacacgg tcaacggagc gctttccgag
     3781 gtaccttcgc aaggtttgtt tttagcgata ggtcatcagc ccaatacgca tttattcaat
     3841 ggcattcttg atatggatga aagcggctat ctgatcacgc aaggtaaatc aacaaaaaca
     3901 aatattgaag gtgtttttgc gtgtggcgat gttcaggatc aaacctatcg ccaagcaatc
     3961 acagctgccg gttccggatg tatggccgcc attgatgctg aaagattttt aggtcattaa
     4021 taataaaaac acatatctgg agaatcaaaa tgttgagagg tcttattact tgtttaacgg
     4081 ctctatcgct ttcgtttgtt gtcagctgca gttcgggctc caagatgtat cagcacagcg
     4141 atctgcaaaa taaacaatcc ggtctttccg gtcgttccgc tacagtacat attgccggta
     4201 tagattttga ctgcaaaatt gtcggcttaa ataaagacgt tttgattgta caacagtcaa
     4261 cacttcgaag cgaaattccg cttgatgcga tatctaagtt ggaggttcga ggttccatgt
     4321 cacacaagac taaaatgctc atcggcggcg gcgccggtct tttagtcggt acaggcgcag
     4381 gcctgattgt aggcccgaaa gtttctgaaa ctaaaggagc taaatcgtta gccggacctt
     4441 tgcctatgct tatgattata ggaggaaccg taggcggtgg tttcctcggt tcttcactgg
     4501 caaaaaatga tgaatatgta tttaatgccg aaatcaaacc gtattctatt cacacagaaa
     4561 taggtacaga agcaacacct gctgaactcg ttgcgtatgg catttttgac gacctatcta
     4621 cgggcgctga tgaaaaaata cttcaagttc aggtgtttca attgaaagat aaccgatatt
     4681 ttatcatgta cgatgaaagt tacctcggcg cgtaccgcgt aggcggtaaa atcgtcaatg
     4741 aagaatacat taaaaaacaa gctgaaaaag tcagtaagaa gcaataatga tatatcgtct
     4801 tattttaatt ttaggttttc ttattggttg taaacctaaa acaccatctc ctctggaaac
     4861 catttctaac gctgtgatga gtgaatctga tatgcaagga gaagcagatt ctgccagcgc
     4921 gctgtggact aagaaagtat gggatgcgct aggcggcaaa cagcggttcg atgaggtgcg
     4981 atatatttca tttgattttt cggttatttc agacggtaag gaagtggcaa aacaccgtca
     5041 tgtatgggat aaacacagta accgttatcg tgtcgaaggt aaaaaccgag caggtgaagt
     5101 ttattcaatt ctattttcag atttaaatga aaaaaaaggg acggcatggg taaacggtca
     5161 aatccaaagc ggtgatgctt tgaagaagca actggattac ggatatggac gttatatcaa
     5221 tgacacatat tggctgatca tgccctggaa gtggcgcgat catggtgtcc gtttacgtta
     5281 cgacggcact caaaaggatt caataaccgg caaagcatta caaacgctgc atttgtcttt
     5341 tgctcaagtt ggtttaacgc ccggcgatca gtattgggtt atgattgatc ccactacaag
     5401 ccgaatggaa agctggaagt ttttactcca gggtctgggt gcagaaaaag ggcactacct
     5461 atggcaggat tggatttccg taaacggaat ttctttttct actaccaaaa taagcgacga
     5521 cgaaaaaaga aaaattctta ccaacaatat tcgtacatac gaagttttgt ccgatagtgt
     5581 ctttttgttc tcagattata cgatttctgc ccgtgattaa tgattggatt acaggtacaa
     5641 cgacccttta tggcattatg gggtatccgg tcagtcattc tttatcgccg attattcaca
     5701 atacagcttt tcattattta aaaatcgcat cctgttacgt tccatggaac gtttttcctg
     5761 aaaaatttga aacagctctc cgtggcgctc aagcgttggg cattaaaggc cttaatatta
     5821 ccatccctca taaagaaaca gctttatatc tatgcgacat ggtgtcggac gaagcccgag
     5881 ccatcggagc agtcaatacg attgttttta cagagaactt gatcagaggc tataacacgg
     5941 atgcctatgg atttttccga gcattacaaa catgcaatat agaccagaaa aataagaaca
     6001 ttctcatttt aggtgctggc ggcggagcgc gcgcggtggc ctatgcgttg ggtaattttt
     6061 ttccggaatc aacgatattt atatctaatc gtactacaga aaaagccaac aaacttgtca
     6121 aacattttaa taacatgttt gaaaaaaatg atttgtttag atcggtgacg gcttcagata
     6181 tgaaaacaat tcagttttcg ttagtcgtta ataccacgcc gccgcagtct gagcattttg
     6241 atgagcaatt atttaaaaga tatacgggta cagctattga tatcgcatat tctatcaatg
     6301 atacgccatt tttatctatg gccaaaaaac accaatggat tataagcgat ggtttggaaa
     6361 tgcttttgtt tcaggccgct caatcattta agctatggac taatgaagaa atgcctatgg
     6421 atgctgttcg cagggttcta tattcgtaca tcgggaggtc gttatgaatc tggataacga
     6481 aactaaaaaa actcttatcc atatagcccg catgacgata aatgctgccg taaagcgtac
     6541 aaaagaacca aattatgaaa aagatgtcgc gacgttcccg atattaaaag agaaagccgg
     6601 cgcatttgta acgatagaga agcgcgggca tttacgcggg tgcattgggt atattgaagc
     6661 gagggaaccg ctgtacaaaa cagtaattat ggctgctcgg tcggcggcct taaatgatga
     6721 tcgttttgga tcgcttcagc tggatgagtt aaatgaaatt gagttagaaa tatccgtttt
     6781 atcaacattt tctcgtgttg aagatatcaa cgatattatg gtcgggacgc acgggcttat
     6841 ggttgaaagt ggtttctatc acggattgct tttacctcag gttgcaaccg aatatcaatg
     6901 ggatcgtata acgtttatcg aacaaacatg ccacaaagcc ggtctaaaca aacatgcatg
     6961 gaaagaaaaa acaacggtta taaagtcctt tacggcgata gtttttaatg aaagagatat
     7021 ttagtttatg taccgagtat ttttgatatt aatattatgt ttttgttgtg aatttaatgc
     7081 ttatgcccgt tcagggggat tgcaggtgtt ttgtttagct ccggctgtgt atagtaaaaa
     7141 tcgttcggaa agcgaaatca gcgaaaaaat tcagcaaaaa ataaattact acagcctgtg
     7201 ttatagtcaa aattttgaca ataaaactgc gacttccttc aaagttcgtt tccgttttgt
     7261 aatcaaaaac gatggatcaa tagttgacat tacaaccgtc ttttcgggca ctaaaaatca
     7321 agcttttatc gaatgcctta ctaatatgtt aaaacaaatt tatttccaaa aaatcggaac
     7381 tgaagccggt gacactatcg ttgaacagtc tatacttttc aaaatccaat cataaaatag
     7441 acacgattct atttgaattt ttacatttcg gtgactaata tcgaagtgcg gaataatttc
     7501 ttacctaact atcggccata cgcatgaaat taatcagaat tccattttta tcacttatcg
     7561 ttatttgggg ctgctctcag gttcagcttt ccacacaaaa tgacaaagca cggcattatt
     7621 atctggaagg attaaaatta gaaaagaaaa actctgatgc gatacacgcg gctctggaaa
     7681 attataaaaa cgcgattcgt gaagatgcga ctttttatga agcctacgtg actattgctt
     7741 ctttgtatga aaagcaggga agttatcgaa aagcggcatt gtatttcgaa aaagcactag
     7801 cgttgcgtca tgaaccgggt cgagctgcgc ttccgtatgc gcgtactttg tacaatctta
     7861 accgctttga agaaagttat gaattggcaa agtactacaa tgagaactat ccttcggatg
     7921 cacaaggctt atcttttttg gctgatgcgg cacgcgcgct gaataaaccg ggtatagaaa
     7981 gcgatctttt gcgtcttacc aaaatggatt cagccaatgc ttatgcttgg agttccctcg
     8041 cgcaatatta ctatgatcaa ggcgattttg cgatggcgat cccgatttat gatcggatgt
     8101 tacgcgaaaa gctgcatgtt acgaccgata tgcgatatga atatgccctg tgtcaacttc
     8161 atgttggttt gtggaatgat gctttgccac atctagaaca ggtcattcgc gaggggatca
     8221 atgacaaata tcctccccac ttggctcagg ctgtaagact tattatttca ggcacattca
     8281 aagaagaagc actcaatttg tttctgaaag ccatgattgc tttcgaatcg gtacaaaacg
     8341 ggcatacgga ccgtcaggag ggttacggtt tagcaaaaca atccctaaca aaatcattgg
     8401 aaattgaacc gaatttcttt ttatcccgcc atttagcggc tcggcttttg tatgttatgg
     8461 aagaatatga cgaagcgttt attaattatg agtggctgac caaaaataac atggccgatt
     8521 cagatgattt tgcaaacatg ggtttccttt tgttcaaaaa aaaccagatg aaagaggcgc
     8581 aggcatatta cgaaaaatcg ctttctataa aacctgacca accctcagta aaaagttctc
     8641 ttgagaccat tcgacgaatc aatgaaggca agatcagtcc acaagcgtat gtatatttcg
     8701 ataaagcctt agcacacaca agtggctttg acagtacatc atattatctc cgtttagcga
     8761 tcgctgccga ttcattgtac ccggaagcat atctacaatt aggattgctc caatatcgga
     8821 acgggcaata taaagcggcg gagcctacat tacttcacgg attgcagcta gcgtataccg
     8881 ataaattaaa aaagaatttc cattacaatc tggctttggt ttactcacgg ttggattggc
     8941 acgataagag cattcaacaa tttctgcagt gtttacggat agacacggcg gacggggatg
     9001 cattgtatta tctttcaaaa gtatatgccg ataaatccga ttatgaaaat gcggtttcaa
     9061 tctatgaaac tttatcgcgt cgagtacctg aatattttgt gccggacgaa aacgatctgc
     9121 agcaagccgg tataggtcaa ttttctcagt ccatcggtcc ctcgcgtttt tactttgacg
     9181 actccttaca tatcggacaa accaatactt accgattaaa aataaaatcc aaaaatgatg
     9241 cattattagg tgcagacgcc aatggtgatc tctctcgtga aataattatc acgttccaag
     9301 agcaagtact agacattacc tcttacggtg tcgttgagtt cgcattagat attcttgaaa
     9361 tcaatggtta tgcgattgcg gcaaaagaaa aagcaattca ggggcaaagg ttttatctca
     9421 gaatttccga tatctacgga gtgacaaata tatatggatt gatggaagaa gatcctgcgg
     9481 gtttatctag attagtcata gccgttatgg aggatttaca cggtaatttt acacgccgtg
     9541 tgatttcgga aggtgaaata tggcgttccg gtcagaatat ctttaagctc ggcaatcttg
     9601 atgctgttac tgaactggat gatattaaca atcagattgc ttacgggaaa aagtattacg
     9661 gtgttcttgg gagttatgat gcggctcggt atggtgaaac aggatatgtt acgattcaga
     9721 ataaaggtga atcggagttc gaatttgaca tacaaaaacg cctgattcgc aaacttaaaa
     9781 atgtctttgt gaccaaagaa tttcgtgacg caacggctaa attgactcag caagacgctt
     9841 catatgctgt tgatttaatt catacgacga ttgaaaacct cgaaaaacca aaaaaagttg
     9901 tacttgaaaa tataccctac gtccgacagc acggccctca atgtgccgca gcgtcgctgt
     9961 ctatggtttt gtcgtattat aaacaatcca ttgaccaaga cgaaatttat gaaaaaataa
    10021 aaagtgacta tgcgggagca cagtccaatg atatcattca ttatcccaga agcttaggaa
    10081 aatataaatc atttggttat gtcggtactt tagatgacct caagcggcgc attgatcagg
    10141 gaatacccgt gttagtattt ttgagtcctt ttggttttgg tcatgtcgtc gttgttatcg
    10201 gatatgatga aacaaaacat cagatcatta tgcatgatcc tactgtagca aataatcacg
    10261 ccattccgta cgatgatttt ttaagagagt ggcaacagtc cggcaatgaa tgtgcgatcg
    10321 tcttacccct ggaaacgccg gtagttgtga ccgaagagcc gctgaattcg tatcaggccg
    10381 ttgaaaaaaa atgggagggt gataaagctt ccggagttcg aaattttgat aaggcgatac
    10441 aactttatcg tgaatccttg cgtctaaagc caaactatga aggcgcgatg gaaggaatga
    10501 tgcttttgca tcttcagaag gatgaatttg ataaagcgtc ggtcataatg gatacgcttc
    10561 ttcagatgcg gcctaatagt attgacttga ttttaagacg cgcgggatta ttattgtccc
    10621 aatatgatta tgataaggtc ttacaaataa cgcgacgcgc caaacagata gatgaatcca
    10681 atatcacaaa ttatctgtat tcagccagtg ctttgttttc acaaaagaag tataacgaag
    10741 ccatgaatga gctcaagcag gccattcgta tcaatccact catcagtcaa cctcgaacat
    10801 tttatgccag tattttggct gaaacgggtg attttgaaaa agctcatgaa caaatgaatt
    10861 tcgctttacg atatgaaccg gaaaatattg gtaactattt gagtgttgca ggtctttatc
    10921 agacagaaat caatgtaaac tttttgacgg cacaagaaaa ggccgatcgt ataaaaaaag
    10981 ccattgaagc gcttgatata gtaaagcaag ccaatccttc gcttccgaat cttgaccaaa
    11041 tttatgccga tatttatacg gctgtggatg atttcaaacg cggcgattct cttatgtttg
    11101 aaaatatcag gaaatatcct gaggaaaacg gggcttataa caatatagct tggcggtatg
    11161 cggtaagtgg aataaaactt gatgtggctg aaaaatattc gcagaaatcg atagaattat
    11221 cacagaggaa cccatattat ttcgatacta tagggtggat tcatctcaaa atggctgttc
    11281 aagttcgtaa aatttatcct gattccgccg aatcatttat gaaaaaagcc gaatctgaat
    11341 ttttagctac tattgaatat gatatgtaca gcgattttgc atacaggcat ttgggtgtat
    11401 tatatgctgt ttggggtaaa ctagcgcagg ccacgacaga atttgaaaaa gtagcggcaa
    11461 tgatgcccga caaagctcgg atatatgccg agataggtca ggattgtgag gaattggggc
    11521 tatatgaaat ttctatgaat tattatcgta aagcgattga tattcgacca aactttgcgt
    11581 atgccaaata tcgcatggct tacatactag cgatcaaaat gaacgactat accaaagcag
    11641 aatcctatat tttagaggca atacaggtag attcggtcaa ttttctttat acgggtatgc
    11701 aagggatcat tgcgttccta aaacaagata ttcctgtcgc aattcgtatt ttggataagg
    11761 tatgctttaa tcaatcagcc tttactgacg aggaagccgc tataaataac tactattttg
    11821 gtatggcact actaaagacc aatcaaattg cacaggccaa aacgcaattc atggaatacc
    11881 tgagacgtag tccggacggt gaatattctg aaatcgtcaa aaagaggatc aaataaatac
    11941 ggagttaata gtatgaaaaa attaggttta attggacttg gtatattcgc tgtttggctg
    12001 tcttcttgta gtgtagccaa acaaattgat atggcgaaaa cgtttgccaa atgtgatttt
    12061 tccgttcaaa atatcgaaca catgcaactg gctgatatta aaatacaaaa tgtaaagtca
    12121 gccaaggatt tgagtttaaa aaaagccgca caattaaccg acgcgctaac caaaaaaacg
    12181 ttacctttga catttacaat taacttgtct gtcgaaaatc ctaatgaaaa accggccgga
    12241 atgaatcgac tcgaatggat tgttcttata gataacaatg aaatagttag gggtgccttg
    12301 gatcagatag tagaaatttt accggattca gatgcgacgg taccgcttaa tatcgaaatg
    12361 gacttaaaga aatcatttag taatgaatcg gccgaaagtt tagtaaaatt ggcatttaat
    12421 ttggccggtg aaggtgataa acctacgcgt ataaccgtca aagttaagcc gactattaac
    12481 gtagcgggat taaatattga atatccgggt tatatagacg tacatacaaa aatcaactca
    12541 gaacagggta aacaattgag aaagaaagcc gctaaaaagg ctaaaaaata aactaggact
    12601 aagtctggcg acctagtcct atcggttctc actataagga gggaagtgcg tgaggcttta
    12661 atacatattc cttataactc atatattata aaattatttc gccaatgcaa gtgttttatg
    12721 gtaaaaaaat agggttgaat ggtacattca accctatcgg ttctcattat caagaaagga
    12781 gataaagcgg gcaaattaga ttttcattaa agcggcttta ggagcatttt caattaacgt
    12841 atgttctttg attttatcta agagattttt catgcgaatg ggtttcataa gtatttcgat
    12901 gacaccgcaa ctttttttat cggaaacgga gatcatttct gtttcggtag ccgtatgcaa
    12961 ataacccgta acaatgaaaa taggcacggt tttatttatt tttcggatgt gttcaataat
    13021 ttctgttccc gacatgacgg gtaaacacaa atcagtaata actaaatcta tcttcatgga
    13081 gccgacaata cttaacgcat caagcccatt ttcaacccgt atcacactac aatgtaatgt
    13141 ctcaaccatc atagctatag tcgaaccggc aatgtcatca tcttccacta ataaaacagt
    13201 tggctttttt gaattcgaaa tcagatcgat ctcttgatgt gtagatgtga gttgctgtaa
    13261 attaggcttt tgtgtttttt ctgttttcaa aattttatta gtattgatta gcttttgaga
    13321 accatattat ttaactgcat aaataatgcc aatatttttg atgcgatgct ttattttaaa
    13381 atattaataa ttaattactt atatttttta ttcttattga tagaagtaaa ttaataatta
    13441 ggcgtcaaat aattgacgtc aacaataata aattttgaaa gtttgattta ttttgaatta
    13501 aaaaaatgaa agcttatatt taaaccttaa aatgattaaa gaaaggcaag acatgaaaaa
    13561 acaaatcttt attttagctg ggatgttatg cttatcaaat ttttcaatta gctcaaagct
    13621 aatggcgcag tcggggatag attcaattga ttattggaaa actcaagctg tggaaaatat
    13681 tcgagaattt gaaagattgc ctgtcacagt aaaggctgtt tctttgatat ttggagataa
    13741 aatatcaaga gctaaaaaaa acataattcg cgcacttgtg gttaaaccag aagatgttga
    13801 atccaaatat atttatgcta tcgtacaaag gtatgaatcc cagtttgatg atgccgaaaa
    13861 aaatattaag tcaattattg acgttaatga tcggttttct gcctatggat ttccgaatgt
    13921 atggattcaa cttggttata tttataaaag taaaatggaa aatgaccttg ctatagatgc
    13981 ctttaaacaa ggtgcactca tagatttgaa cgatacatgg ccgcttattg agctttcatt
    14041 tgttttttct gaaatcggta aagatgtcga agcgtgtgaa gcgttttacg gtggactaaa
    14101 cgaaattaat gatccgcaaa aaatcgaaaa attatttgtt gattgcagag atattgcttc
    14161 agagaaagaa attaagatat gggaggattc attaaaagcc aacaaggaaa aactagagtt
    14221 tttaaaaatt ttttggttaa aaagagatcc caacccgttg gatccagtaa atcagaggct
    14281 gctagagcat tacaaaaggc ttattttcgc tcgttctaat ttcagtaagt cgctcagtcc
    14341 atggtatgat gatcgcggtc agacttatat ccgtttggga aaaccggata taagatatat
    14401 tggacgtact gcagaagctg taaaggaaaa tgaatcttgg gtgtatgata gaataaaatc
    14461 ggggctctat ttcgatttta tagaattaaa tggaagttat aaacttggat cattgtttga
    14521 tgctttgaat atgtcaagcc gagctagctc gatggttgag attttcaacg aaagatcaca
    14581 cataaatcca tattatcaac gcatggctga tcgcatcaaa acacaaactg acgttgttca
    14641 tgatagagca gttgaggcgt ttaacaatac ggtttcaact aaaccaggag aaggttctga
    14701 aagccatttg aatgcggtag atcgcagttc tttcgcatca acctttctaa atactaacaa
    14761 ttactctcaa gattacttag ccaatataga atactcaaaa aaacaaaatt tcatatttga
    14821 tgtcggtgca ccgcatttgc ccatgaattg caattttgca tcgttccgga gtgttaataa
    14881 aaaagattca agattggagt tttattatac agttccgttt aaagtgctca attttgcgcc
    14941 aagcattacg caagtggaca agttcaatac atcaattaag cttcatatga aattatatga
    15001 tttgaaatat aatgaagtga aatctgttga gagagatttt gacatatcgg catttggaaa
    15061 agaaacaaca tcgcactttt tcttagatca gtttgatgcg gatatcactc cgggaaaata
    15121 tattgcagcc gttgaaatga gaaataacga aaaggatcgc gtcggtatat atcaattcgt
    15181 tcttaatgtt cgggattata attcggactc attattggtc agcgatatag aaatcgctca
    15241 atatgttgac caaactttaa ctaaagacaa atatcttaaa ccacagtcga atcttaaagt
    15301 tgtgcccaac ccggctgccg gattagttaa taccaagcca ttaaccatct actatgaaat
    15361 ctataattta gccaagaacg cagataatag gacatcgtat caggtatcgt actcaattaa
    15421 aatgcttgag agcaatcaaa gttttttgag ctccgtagcc ggtgttttta ccggaaagaa
    15481 agaagcatcg acgtcttctg ttactgtcaa agaaggtaag tccgtgaatg aaaaagaata
    15541 tatagcgttt gatatcgcgg atctgccgac cggagtcgct gctcttgaag ttcgtgtcaa
    15601 agatctaaac tccggtaaag aagcggtaag tgcgactaac atcacaatta caagcgcccg
    15661 tgaggagcag aataatgcat cttctgagga aagtgttaaa gaaaaataat gttgaagtac
    15721 aaattctata tttgttgcgc tcatattatt gcactgggcc ttgctgctca aacctatgaa
    15781 acgcaatcat cgccgatagt cattaaaaaa caaaatgaaa tggttgtttc aaaaccatct
    15841 acattgaaag caataggttt atccatctta atgcccggag ccggcgaact gtatttgaag
    15901 aaaggaaaaa gaagttattg ggcagcaggt ctctttatgg ttgcagaaac cgcaacatgg
    15961 ataacttatt tttcttaccg caatgcaggg cttaaaaaag aaaaagaata cattagatat
    16021 gctgataaaa attggaatgt agatgagtac cttcgttttt tagaaacaac tttgcagctt
    16081 tcagatggct atttaggtcg caaatcatca ggtatagata aagataaact tgaagttgcc
    16141 gaaaacgaat ggggaagcct tagcggtata tcagtgcatc atttatacaa aagcggacgc
    16201 caacaatatt atgaaatgat ctataagtat cctgagcagt ttggacaagg gtggagtgat
    16261 gcgaattggg atttggttga tatccccaat cgttctactg gatatacgcc ttataattta
    16321 acggatcgta tgaaatatta tcgaagcatg cgtgtaaaaa gcaatgcttg gttagaaaac
    16381 gctagaaatg caacgggtta tatgcttgcg aatagggtac ttagtgtatt agatattgtg
    16441 tggattacta agaagtacaa ggcttctaat tctacagaat ctgttcaaat gggtattcgt
    16501 atcaagccgg tcaaggatct taatttaagg ttaaaagcat atccatcgct tgaggtaacc
    16561 ttctaatgcc tacttcttcc ttaaagtctc ctaatctttc ttttgggatc ataaaaagcg
    16621 cattcaatca aaatgtagta aatttattgt ttgaaggttt ggcgcgaggg tttcaagatg
    16681 cgggtattga tcaaaaatac attgatgttt atgatgcccc gggcgctatg gaactccctg
    16741 tctcggcaaa acatttatgc gaaacaggta aatattccgc agtcattgcc ttaggttgtg
    16801 taataagggg gaatacaccg cattttgaat acgtttcagc cgaaactacg cgcggactat
    16861 cacaagtaag tctaacaacc ggcattcccg ttattttcgg tgtattaacg gttaatacct
    16921 tagaagatgc aatggatcgc gtcaaaaatg ataaatcgaa taaagggtat gaagctgcga
    16981 tggccgccgt cgaaatggcc aagttaattc taaggatcaa atcatcccgc caataatgcc
    17041 tttatgatca cttcgctaac aattaagaat ttcgcgctta tcgaggatgc tgaaattatc
    17101 tttggtaagg gattgaatat tattaccggt gaaacgggtg caggaaagtc cattctattg
    17161 ggtgcaattt cgatgatatt aggcgaaagg gcttcgacgg atcatattcg taacggcgct
    17221 gataaagcga taattgaagg tatctttcac actaaaccta atcgcttttt aaaagcaatc
    17281 ttcactgaga atgacctggg atggagcgat gaacttgtat tgcgccgcga aatttcgaca
    17341 cgcggacaaa atcgttgttt tgtaaatgac aatcttatca ctactggatt gcttaaatcc
    17401 atcggtgatt atctagtgga tctgcatggc caacacgaac atcaatcgct cattcgccaa
    17461 gaattccata tcgaattttt ggatgcattt gccaacaacg ctgaaaatgt acaacaactg
    17521 actgaattat ataaatcagt tacggcactc caacgtcgca ttcatgaaat tgaaaccaat
    17581 aaacagagca taattgataa aaaagaatac tatagtttcc aacttaaaga aatcgaagaa
    17641 gtcaaccctc aaccgggcga ggatgaatca ttaatacaag aagaaaaatt agcttcgaat
    17701 tcggagaaaa tatatgaatt gagtcacagc gcgtaccaag atttatacga atccgatggg
    17761 gccattattg aacgtttgga acaagtaaaa tcaaaactta atcaacttag ttcgtttgat
    17821 gagaggctga ttgattatca gaaagatttc gatagtgccg taattgctat cgcggaaatt
    17881 gcccgcgttt tgggtgaata taatcataaa atcgtatttg atcctgaacg tttagatctc
    17941 atgcgagcgc gattgatgaa tttacaaaaa ttgaaacgta aatacggttc tattgaagac
    18001 ataatccgag ccaaagaaga gtatcgaggc tatgtttcat tatcggaaaa ttatgatgaa
    18061 cagcttctgg ctttaaaaaa agaactaaat aaatctattg aaagctacgg taattgcgca
    18121 catgcggttt ctgcggcacg aatcaaagcc gctgaaaagc ttgataaacg tgttgtgcaa
    18181 gagttggtta agctcggcat agaaaaagca caatttaaaa ccatgatcac gcagatcgag
    18241 gcaccgaatg gatggtttat ttcgaaagat aaatccattc aagccacggc gcaaggtatc
    18301 gatcatgtag aatttttcat ttcggctaat gccggggaat cactaaaacc gctcgtcaaa
    18361 gttgcttccg gcggcgaaat ttcgcgtatt atgcttagtt taaaaacagc cttagcgcaa
    18421 tcagataaaa ttcccaccat gatatttgac gaaattgatg tcggcatatc cgggcgtatt
    18481 gcacaagcgg ttggaattgc attaagtgag ctgtcagagt ctcatcaaat cattagcatc
    18541 acgcacttac ctcaaatagc tgcattagcc caaacacatt ttagcgttca aaagcaaacg
    18601 caggggcatc atacaacaac aacgattcgg caactaaagg aaaatgaaag agaaacagaa
    18661 attgccaaac ttctaggcgg agcaaccatc actgatgcgg ccattaaaaa cgcacgggat
    18721 ctcatgcgtc aaaaggtcgg ttttctatag ccgtgttttt acttccaaat tgcgtccgat
    18781 ggttattgta tgaatcatct tattgaaata aagaaaagct taaaaagggc ctcaacagtt
    18841 gaagctaagt tcgctactct caaagcggtt cctacagcta aaggcgttta cggcgttcgt
    18901 atgccggtac tcaataatct ggctaaaaca tataaaactt gcgattttaa actgattaat
    18961 gagttatgga aatccggaaa atttgaagaa cgcatgttat ccgctaaaat tatcggattc
    19021 aaagctaaaa aagaaccggt gaagacattg tctacaatcc gaaaattctc cgcatcggtt
    19081 gaagattggg cgatctgcga ttgtttgagc tcccaaagta ttcgatctat tgctttatcg
    19141 gaacaaaaaa gaattttcca gctttcagag ctgctgatca catccaaaaa aatatggcaa
    19201 agacgaatgg ctctagtttt attggaatat tacacgcgtt ttccgcaatt tcatccgtat
    19261 attcacaacg ctattgccag tcttgaaaat gagaaagaat attacgtttc taaagccatc
    19321 cattggctta agcgtaatat gttaaaaaaa cgttaaccaa caaacctaca ggtctattga
    19381 ctatgtctga tttaaagcat ctgttccaaa ataatcttca ttggtctgaa aacattcgag
    19441 cacaaaagcc ggactttttt gataagcttt cccatcaaca agcgccggaa tatttatgga
    19501 ttggatgttc cgacagcaga gttccggcca acgaaattgt aggacttttg ccgggtgaaa
    19561 tgtttgttca tcgtaacgtg gctaatgtgg tggtgcatac tgatttcaac tgtttatcgg
    19621 taatcgaata cgccgtcgaa gtgcttaaag taaaacatat catcgtgtgc ggacactatg
    19681 gatgtggtgg agtggcggca gcccttcagg atagtcgatt gggactgatc gataattggt
    19741 tacggcatat tcgtgaggtt tggaaaaaat acgaaaccga gttgaatacc atatcggatc
    19801 cgcaagaaag acatcatcgt ttatgtgaat tgaatgtcgt cgagcaggtt gcaaatgtat
    19861 gtaatacgac gatcgtacaa aaagcatggg atcgtaaaca ggatcttacg atacacggct
    19921 ggatttacgg tttacaaaat ggattgatta gggacctgaa ggtatctaag gataatgtat
    19981 agaacgaagt aaaaatgatg tgcataataa aaaaaccttc tctacagaga aggttttttc
    20041 gtggagacaa ggggggtcga acccctgacc tcaagactgc cagccttgcg ctctcccaac
    20101 tgagctatgt ccccgtaaaa ggtgcgcgta atctatacga gcaagtatga aaaagcaagt
    20161 tttttttata ccaaatccat aattgagccg aaatccggat cgtataatct tttgaaagtt
    20221 cgcaaagcga atccgtccgt catacccgcg acataatcac atatcaaccg agcacgtttc
    20281 tttaaatctt tttcattacg aaccggtcct tctgtatggt ccggcaacaa agtcattgta
    20341 tttcggttat tgtttccgag gtagtgcttt tcgaaagcat caaacatttt tcttaaaata
    20401 taatccccct taaattctag ttgatagatt tgcggtgaaa aaaatacaag ctctcttgaa
    20461 atacgaccgt ataaatcaat ttctgcacgt ttttctttat ttatagaaag gtaaaacgca
    20521 tggcgatttg ttttatcgga cataaaattt tttatcggcg acaaattaac ggcgcgtata
    20581 cagtcaccaa ttttacgact gcaaaaacgt gcaatttgac gctcagacat agctttcaac
    20641 agattcgaaa tcaaaacgtt ttcatcatta gtaaatgatg atttagcggc ccaagactca
    20701 attttggctt gtgttatcag ccccgagcta ataccgtcca ataaatcgtt aatcgaataa
    20761 gctgtgtcat ccgcccaatc catgatttgg cattctatgc tacgaaactt attatgatcc
    20821 acatctttag gtatatcgcg gtgatcaaaa atgaattgaa catatggcca ttgttcatca
    20881 tacaaaaaat gatttttggg cggttgattg ttattatgac tatgcgagta aagcttttta
    20941 tacttcatca caccatctgt caaagccctt gtgggattca ttccttcgcg acctgaagcg
    21001 gcgctataaa ttgtttcggt taacattcgt agcgtttgtg cgttaccctc aaaccctcca
    21061 tacggttgca tcaatttatt aattgtcgtt tctccggcat gcccaaaagg cgggtggcct
    21121 atatcatgag cgaggcatat cgcttcgaca agatcagagt caatgtaaaa atcatcctta
    21181 agttctttgg attcttcttt cagataatgg cacaatgatc ggccgatttg cgccacttct
    21241 aaagaatgag ttagtcgtgt tcgatagaaa tcatactcgc cagaaaaaaa tacttgtgtt
    21301 ttggattgca atcttcgaaa agaggatgtg taaataatac gatcacgatc ttgttgaaaa
    21361 ggagttctct cgtaaagatg gcgtccactt acgccgtcaa ttaattgaat gtcaaattca
    21421 ttgtaaaatg tattagttat cattttccct catgaataat gttccggcca tcggttacat
    21481 agatttttat aggagcaata tacacaattc tttttttcat ctgtttgttt aaatggtcgt
    21541 tcgttattga gtaactctag agcaatactt cgaaaatatt ctaaagatgt tttttcaatt
    21601 tcagtctcaa tcgagtgtat ctcattttta tcataagtta gcggcaaata atattctgca
    21661 ggtgatccac gcaaagcaac gatcatgctt cggtattttt tatatttatt cccggaatgt
    21721 gaacggtgta gtatgatata tgttagaagt tgcaacgctt tatcagaatc acttttaact
    21781 gaaagatcag caatggttgt tggtaattgg acgtgttttt tctgggctga accagtttta
    21841 tagtctatga tttcaacaac atcgtcaata aggtcaatac gatccgcttt tccatgcagg
    21901 cgcattgaga atagttgacc ttcaattggg acatctattg aagtggacat aagtgattcg
    21961 acagatagga cccatcgatt tgtattatcg tgtgaaagac ggtctttttc attgttcaaa
    22021 aaacgttgaa tgtgctgttg agcaatagcg aatgttattt tattttttcc actgtggaca
    22081 tccggattgc ccatatcatc caatgcattt tggatcgctg tatccacatc attcagcatt
    22141 gaactaatgt ctttgg
//
LOCUS       DAOQNQ010000022        21157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_849_length_21157_cov_7.031561, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000022 DAOQNQ010000000
VERSION     DAOQNQ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21157
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_849_length_21157_cov_7.031561"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1814)
                     /locus_tag="PK754_02835"
     CDS             complement(<1..1814)
                     /locus_tag="PK754_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB08469.1"
                     /translation="MKWFRWIGIIFLLGCSSIFAGNSNETENKTIGVISGRVTEENSG
                     APLAYANVAVAGTILGAVSDAEGFFKIIKVPQGIYTIVATYIGYEKKALDNVKVKTGE
                     TTHIEVRLAVSEITSEEIVVSATRKETTANLAPASVGVITAEEMQKKNISTFDQAIES
                     APSVQLTRSSGSNVQAVSIRGSSEVAGAGVGNRVLLLIDGRPALSPESGGALWNLVPT
                     NSIERIEVVKGAYSSLYGSSAMGGVINVITKNPSPVAVTRIHTEYGFYEKAPGWTGYD
                     TYNAFNTIEVAHSRRVGNVSYLIEAGHRHNDGHRQNTEFDMVNLYGKTAYHFANNKNL
                     QLAMNYNHVANDYPATWLSFLQPYHVAPAKLDNRQQRSEFSTDLYYYSIPTSELKYSS
                     RFYYYTNNSEYTFWKDSSRMGNERIRQTSINNHRIGNVTQFDYYMSERHYMISGMEIQ
                     LDFVDARPANILYGKHTAYNVAAYSQDEIKLSDRLTTTFGLRYDYNKIVDGYGEGNFS
                     PKISTVYEIQKDWSVRALVAQAFRNPSIAERYIKYEQGGNLHFHQNPNLKSEKLYASF
                     EVGTKFKITDYTSYDISLFWNEYKNMIAYIADTAVAYQG"
     gene            complement(1816..3078)
                     /locus_tag="PK754_02840"
     CDS             complement(1816..3078)
                     /locus_tag="PK754_02840"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNB08470.1"
                     /translation="MKRHIRNKILRLSLIASTLFFLIKPVYAQIDASILRRHVQKLSG
                     DEMEGRGTGEPGEAEARGYIASEFKRYGLAAYGNDKGFLQKFEFRPRNGNNPHAAIDT
                     AQPARTAYNVVAYLDNGAENTIIIGAHYDHLGLGRQGGSLDPNPEGKIHNGADDNASG
                     TAGLLELARFYRQNKIKEKYNFLFIAFSGEELGLLGSKAFTANPSIDLNKIHLMINMD
                     MIGRLDSARRLMIYGTGTSPALENLVRNTSHDFQIKTDSSGIGPSDHTSFYLKNIPVL
                     HFFTGSHPDYHKPTDDADKVNYDGQKAVLEYIQRIIEKAEAMPKMAFTATRNNSTASD
                     SPRFKVTLGILPDYAFTGGMRVDGVTDGKPAAVAGLKAGDVIVQIGEHIVFDIQTYMQ
                     ALSKFTKGETTNVKIKRGEDTLDMKVVF"
     gene            complement(3081..4079)
                     /locus_tag="PK754_02845"
     CDS             complement(3081..4079)
                     /locus_tag="PK754_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08471.1"
                     /translation="MAQTPSSEKHLKNLRQLTFGGDNAEAYFSFDGKKLTFQSNNPAW
                     GLSCDQIFMMDIVSADSTYKPKQLSTGLGRTTCSYFLKGDTRIVYASTHLGGKDCPPP
                     PPSRSDRKYLWQIFDTYDIFVTDTEGRILQPLTQTPGYDAEATVSPQGDKIVFTSTRS
                     GDLELWTMNTDGSQLKQVTHDLGYDGGAFFSPDGKKLVFRASRPKTEDEIKEYKELLS
                     QGLVAPSNMEIYTCNVDGSDLRQITQLGKANWAPFFHPSGKKIIFSSNHHSQRGYDFQ
                     LYMINLDGNGLERITHESIFNAFPMFSPDGKRLIFSSNRNNNGTRDTNLFIADWED"
     gene            complement(4212..4790)
                     /locus_tag="PK754_02850"
     CDS             complement(4212..4790)
                     /locus_tag="PK754_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08472.1"
                     /translation="MKKTLLLSLILAIGIMILSGCDDDKKPTTATIKGTVTFKNVSVW
                     KASGSFTKADEDSGEVEVTIFADSVWKNGPGGRVPYGAPYNANNPVVFTRAAGDSTYQ
                     YEIEVEPGTYSALAVGFRTNRAVSSDKKTATLGVHWGMPDSVSHGIYIAPALPYFPGY
                     DYPAPAAITVEAGDELTLNFKADFGFVNSWFH"
     gene            5056..5631
                     /locus_tag="PK754_02855"
     CDS             5056..5631
                     /locus_tag="PK754_02855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNB08473.1"
                     /translation="MTDQEIIAIFQSAEGAKRDQVFGDIVHRYRERLYAIIMGFTNDH
                     DETDDLLQETFIKAYQHLHTFRAESSLYTWLCRIAINLSISYARKRKLRQFFSVDAME
                     EILPSGQDGPDATTEKMELRKHIKRAVDQLSEKQKKVFILRYYDELSHAEIAQITGNS
                     EGTIKANFFHAMNKVQAYLEKHYFEKGKPHA"
     gene            5624..6208
                     /locus_tag="PK754_02860"
     CDS             5624..6208
                     /locus_tag="PK754_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08474.1"
                     /translation="MRDEWVAYHKNKLSPEARRRFDENLISDPSENEAFQKFSRVAEA
                     LESARPDTPEGYWRNFNVHLMDRIAEKRKPRFSFSWKLAIPAIATAALAVFFLVPHNT
                     DDTTAVHPDVWNDLASSLESMETSSAFESGADIQIASEDVEETMVTADEWHDFSSALE
                     SIRFSVNETNADGTVWDKSAKEELLKTLDQTSII"
     gene            6231..6752
                     /locus_tag="PK754_02865"
     CDS             6231..6752
                     /locus_tag="PK754_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08475.1"
                     /translation="MKLFTILGVTLILGTSGVSLHAQEDAEFKERIIEIKKKKLLESL
                     ALSKDKENQFLKVYDEYFDKRRDLNRERKAVMKRIAHMSQLQADDIQGDKVTKAIEEL
                     GYVERKIVDQRQRTVEQLRGTLTEPELAKFVIFEQNFPIRMREMIFDIRARKHKDKDM
                     RMFIPPGEDDLLD"
     gene            complement(6928..8427)
                     /locus_tag="PK754_02870"
     CDS             complement(6928..8427)
                     /locus_tag="PK754_02870"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HNB08476.1"
                     /translation="MRRLFPPSLYNMVSMSGAALSVTSFAVFIFLFLIEITADHSNPY
                     MGIFTFMVAPAFLILGLLMIAYGAWREKKRIARGTPSEGHLPHIDLNDPRHRRATAVF
                     FFGTIILLGLSAFGSMEVLEYSDSVQFCGEVCHNVMHPEYTAYAHSPHARVPCAECHI
                     GSGAEWFVKAKISGAYQVYSVLFNKFSKPIPTPIENLRPAQQTCEQCHWPRHFFSEKQ
                     RALTYYVSDENNTKWTLNLLMRIGGGNELSGNTDGIHWHMNIANEVDYVSTDHKRQEI
                     PWVRIRDKKTGKETVYQSTENKISEAELKNHETRRMDCIDCHNRPSHKYNHPANQVNQ
                     YMQLGWIDPSLPYAKNISIKALEIPYTSTSRAMDSIAIVIRDFYSQNYPDISKHQSAK
                     VDKAIQETQKIYSRNYFPEMRADWRGYPDNIGHMYAAGCFRCHDGKHKSDDGKVISRD
                     CNVCHTLLAQKFENDKQEVSMQGLNYRHPVNVDKAWQEMSCYDCHNDKK"
     gene            complement(8440..10635)
                     /locus_tag="PK754_02875"
     CDS             complement(8440..10635)
                     /locus_tag="PK754_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNB08477.1"
                     /translation="MIFLFAGFSLKAQSNDDCMECHSDESLSKKKAGKKISLFVDEKI
                     RGKSAHAKVNCIACHTGFDPEEIPHKEKITPVNCVSCHNKELTKHAFHPNIMATKGTG
                     WSDDANCKTCHGTHDVASTKVPGNIFHKDNLVNGCGSCHKDVKEDFIHSAHAEAQKRG
                     DKNAPTCIQCHSSNITHIAAGMDSLQLKVAQAKVCMSCHVDNQEVSSKTLLGAQFVRS
                     FEKSVHGQALKNGKAEAANCVDCHGSHTMNKAIVDNSPVNKMHIPETCAKCHKDIAND
                     YNSSIHGNMLHKGSKDAPGCTDCHGEHDILKHDDPKSPVSSRNVSQQVCGTCHASVKL
                     SKKYGIASDRFQTFSDSYHGLSVRGGALEVVNCASCHGAHNIKQAKDSTSTISKANLV
                     KTCGKCHPGAGDRFTIGSVHSSEEKKADDPLLWYVATIYLILIFVVVGGMALHNIIDI
                     HRKVRRKLAIQRGELHEEEHHGRGLYVRMTASERLQHGTMALSFIILVITGFMLRYPD
                     AWWVVGIRSLSEYAFEARSILHRVAGIAMVVVSLYHLWYIIFTVRGRQLVKDLWFCKK
                     DWADAVGILKYNLGISKDKPRLGRFSYVEKSEYWALVWGTIVMALTGAILWFEVQSID
                     MITKLGWDVSRIIHFYEAILATLAIIVWHFYFVIFNPDVYPMSFAWLTGKISEKEMEH
                     EHPLELEAIKKSEEANRPQDEVVRVSQENVSDAKADDTPDENNINNSKN"
     gene            complement(10884..11252)
                     /locus_tag="PK754_02880"
     CDS             complement(10884..11252)
                     /locus_tag="PK754_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNB08478.1"
                     /translation="MKKLMFIAAFGLLVMFVNVAQINAQEAKPADGKAIFEANKCMSC
                     HAVKNIGIEAKKAEGEEAEENKAPDLSGVGLEKDAEFIAKYLQKLEAINGKKHMKKFK
                     GTEDELKTLAAWLAEQKTKQ"
     gene            complement(11472..11840)
                     /locus_tag="PK754_02885"
     CDS             complement(11472..11840)
                     /locus_tag="PK754_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB08479.1"
                     /translation="MKSIVAFAIAFFATILMLSSKAYAQEAKPADGKAVFLSFKCESC
                     HAIKSMAIEAKKAEGEDAGDNKGSDLSGVGLKRDADFIVKYLQKLEAIEGKKHMKKFK
                     GTEEELKMLASWLAELKTKE"
     gene            complement(11925..12710)
                     /locus_tag="PK754_02890"
     CDS             complement(11925..12710)
                     /locus_tag="PK754_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNB08480.1"
                     /translation="MMNVKVINLESLEGFGLHPDSGRNFLIHISSINLWKGLSIALVF
                     HAALITSYYVSAVLLKEDISMRTVRFVDANELTSAAPLEKTAEPVKVDVQMNEIPKVI
                     AGIPIVVPDAEAPSDVTLQTQEEIKTNIAASTFSLGNAGEGEIKVDISESLRGLNESS
                     DEPGMDEFIPVEQAPILLAKVIPEYPEIARQAGIEGKVFLKALVNEEGVVVKVVYIQG
                     DDMFKEAAVNALSQARFKPAINGNRPVKVWISYPFIFRLRTTQ"
     assembly_gap    13001..13100
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13245..13655)
                     /locus_tag="PK754_02895"
     CDS             complement(13245..13655)
                     /locus_tag="PK754_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08481.1"
                     /translation="MTRQLKLPLLILIFAFTACNSDQNDSGPDKRLANTQWRNRLLTF
                     PGNRHYYAFDEAGGGGQFAWPKDSCYVLDSLEVRDGKLISNDKEYAYRLSFGSTLLIV
                     TYPIYYAGDSEDVSYIDVAFERMAYSADTLDICD"
     gene            complement(13746..14915)
                     /locus_tag="PK754_02900"
     CDS             complement(13746..14915)
                     /locus_tag="PK754_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNB08482.1"
                     /translation="MMLKHSYLILICILCSCGGCLDEGGDQLLDLKEVWKVKVTQGLY
                     SYTIPAATTSTVTFATTTGEVHGFSIQSGAKKWATKVGQTSQPIVNRFRAYGGHIYFD
                     YSNRLICFDGESGAIDWQFVKSQTYGTLSDPEPSEEYVFTGDSYTPSGYNEGNIFCVL
                     RGSGQLNWKRRLSEGVNNIAASTDGYVYVTTQGFRMHPERGEVPYGLVYCFNSQTGDL
                     IWSYDVSNRPNAGFVSGAADCRAAIWQDRVFIVNSSGSVFCLNRFTGTEIWIKDLQSG
                     SFAGMTVDDANQRGFVVAGIEFYCLTLDSGNLVWRSNSRIGESAEWEPELFNGVVYNM
                     GSVGFVEARDALNGQLLYQSIKNHETFFRFGSLQDDCFYMLNNNDNAIMKLKPLR"
     gene            complement(14974..15846)
                     /locus_tag="PK754_02905"
     CDS             complement(14974..15846)
                     /locus_tag="PK754_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08483.1"
                     /translation="MNDLRPGSAGIQYLNSNNIETFNRVGIIGLDLDPAHEPYRLAFT
                     QLANDPQLGADIQDFLYAVYAIVAYIYWEYGDWLWDEGYISDAYTAWAIGDIFYASAV
                     DIAYAGYHWKIVNGSIIYSNGDYGWTYSDGVVPDYSQDYPNANRSPRIIYNTNHLLEG
                     RSNRGKDAISLGLNDVGFVHMTPPPAPITVSVTPQPSQAPAGGSYTFTANVANGSGNY
                     TVKWYSYYNEGGGTGGEIAMLKEGFVPPGGGTWTYIGTGPTKVFQPSSKHQLRADATD
                     NVSGQVASYTISWY"
     gene            complement(16090..16386)
                     /locus_tag="PK754_02910"
     CDS             complement(16090..16386)
                     /locus_tag="PK754_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB08484.1"
                     /translation="MKTINHIFICFLFATGLRAQENVVFLHGINSSGGMWTGSYNMIA
                     KLKQSFYMNATNPTRDDQTTFSVQADDVKTSLNPNINNYIAVGHSMGGLAARTY"
     gene            complement(16401..17207)
                     /locus_tag="PK754_02915"
     CDS             complement(16401..17207)
                     /locus_tag="PK754_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08485.1"
                     /translation="MMVSCDDASDKNGPIAPEINAPKKTTATTTISYTATTEVEEDDT
                     FMPYAVPDVSGNTAAGLGPNHTVTYSQNVSLTQTDGITTSMTITFAPLTGAADSLLAL
                     GRPSSIVYDGSTVRMYNRNGVLIPGNPYLPQSLSLNPAGDVQQQSVSSKRCSKPANIF
                     VEPKDLLTTAREQFDAVEKVAGNEWKFTIDRPFGKIELAYDDRLKTITSEKVYRDGEL
                     FLQKSYEFAESQGRKHRSKTKVTKTQKLPGGKQRTRTYTETVSNVSITNN"
     gene            complement(18052..>21157)
                     /locus_tag="PK754_02920"
     CDS             complement(18052..>21157)
                     /locus_tag="PK754_02920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB08486.1"
                     /translation="LDTASLSDNRILKVFKDRSGLIWIGTADGGLCRFDRDKESFVIY
                     RNDPKNSNSINSNRVNFIVEDASGNLWLATAGGLDILDKSTGIFSHQDKSTPIGKALP
                     SRQVNAMLFDTKGRIWIGTNKGLISHELATGKTVKFEYGIGTGKIIHENILSLCEDHG
                     GMIWIGTAEGLNMYDPVTDRFVTFTNNPSDPASLSHNMVWSVFEDHAGVIWVGTQSGL
                     NRYDREEKRFMRFVNDPTDKSSISHSYVNTIFEDNAQRLWFGTYGGGVSRFDPHQQKF
                     SLYVSDAKNKTSLSNNVVWSFYEDVKKQIWVATEYGLNQLDPVKNTFNHYFADASLKN
                     NADNSYNCLLEDSKGNLWVGSYGSGLRRFDRASGRFVASYKHSVDPASLIENRIITLL
                     EDRDGHIWVGTMNGLDLFDPQKNGFIHYQNNPDDPASLRNNVAKVLYQDRSGALWVGT
                     QGGLSVRLPGETAFRHYLHDPRNLSTISHNNIRAITEDTSGTLWIGTTGGLNRFDRIQ
                     STFQHYTTKEGLPSDIIYGLLCDAKNRLWISGNKGLTRFIPSDASGSSGVFRVYDVSD
                     GLQSNEFRNGSAYKAQNGEMYFGGIRGFNRFHPDSVSDNPNIPNVVLTQFKIFEKPVA
                     LGKDINDLPVVELSYKQNFFSFEFAALNYTSSEKNQYAYRLEGFDNGWVNIGTERKAN
                     YTNVDPGTYVFRVKGSNNDLVWNEKGVELKIVIQPPFWQTWWFRIAALALLIFSAVTW
                     YKQRMRRLLHQKQLLEKQVADRTSQLRQKTEQLETTLEDLQKTQSQLVQAEKMSSLGQ
                     MVAGIAHEVNTPRATVDHYVFILYRKLQDAVQSQNPNDMRAALENIQEEIVPPIELAT
                     ERIGEIVEGLLNFARADHKDYRMSDVHKLINSALVIAKNQYKGRIEIIKNYGEIPQIE
                     CFPGQLNQVFMNTIVNAVQATPGQGTITITTEMADEKNIRVRIKDTGMGMSEEVKKKI
                     FDPFFTTKPVGQGTGLGLALAYEIMGKHNGKIDVDTSPGKGTEFIFTLPIEQPKKELG
                     TVLS"
BASE COUNT         5709 a         4969 c         4428 g         5951 t
ORIGIN      
        1 ccttgatacg ccaccgccgt atcggcgata tacgcgatca tgtttttgta ttcattccag
       61 aatagcgaaa tatcgtacga tgtataatcc gtgattttaa atttggtccc gacttcaaac
      121 gacgcgtaga gtttttcgga cttgagatta ggattctgat gaaaatgcaa attaccgccc
      181 tgttcgtatt tgatataacg ttcggcgatg gacggattac gaaacgcctg cgctaccaat
      241 gcacgaaccg accagtcttt ttgaatttca tatacagttg aaatcttagg actaaaatta
      301 ccctcgccat atccatcgac aattttgttg taatcatagc gaagaccgaa tgtcgttgtg
      361 aggcgatcgc tgagtttgat ttcatcttga gaatatgcgg ctacattata cgctgtatgt
      421 ttgccataca atatattggc cggccttgcg tcaacaaaat ccaactgaat ttccatacct
      481 gaaatcatgt agtgccgttc ggacatataa taatcgaact gtgttacatt accgatacga
      541 tgattgttga tcgaagtttg gcgaatacgt tcgttaccca ttcggctcga atctttccaa
      601 aacgtgtact cggaattatt cgtgtaataa tagaatcgcg atgagtattt caattcagaa
      661 gtcggtatcg aataataata taagtctgtg ctaaattcgc tccgttgttg gcgattatcc
      721 agttttgccg gtgcgacatg atacggctgt aaaaaactga gccacgtggc cggatagtca
      781 ttagccacat gattataatt cattgcgagc tgcagatttt tattattagc aaaatgatac
      841 gccgttttac cgtacagatt gaccatatca aattcggtat tttgacgatg accgtcgttg
      901 tggcggtgac cagcctcgat aagatacgat acgttaccga cgcggcgact atgcgccact
      961 tcgatcgtat taaatgcatt atacgtatcg taaccggtcc agccgggagc tttctcataa
     1021 aaaccatatt cggtatgaat ccgtgtcaca gcgaccggtg acggattttt tgtaataaca
     1081 ttgatcacac cgcccattgc cgatgaccca tacagcgagg aataagcgcc tttgactact
     1141 tcaatgcgct cgatggagtt ggtcggaacc aaattccaca acgcaccgcc ggattccggg
     1201 ctcagcgcag gtcgcccgtc tataagcagc aacacacgat tgccgacgcc tgcacccgcc
     1261 acttccgatg aaccgcgaat cgaaaccgcc tgcacattgg atcccgaaga gcgggtcaat
     1321 tgtacactgg gcgcagactc aatcgcctga tcaaaggtcg aaatattttt tttctgcatc
     1381 tcttcggcgg tgatcacacc gacgctggcc ggcgccaaat tggctgtggt ctccttacgt
     1441 gtcgccgaca cgacgatctc ttcagatgtg atttccgaaa cggccaaacg tacctcgata
     1501 tgcgtcgttt cgccggtttt tactttcaca ttatctaagg cttttttttc gtacccgata
     1561 tacgttgcga cgatcgtata tatcccttga ggcactttga ttattttaaa gaacccttcc
     1621 gcatcggaaa cggcaccgag tatagtaccg gcgacggcca cattggcata tgccaacggt
     1681 gcgccggaat tttcttccgt tactcgaccg ctgatcacac cgatcgtttt gttttcggtc
     1741 tcgttactat taccggcaaa tatactagaa caacccaaaa gaaagatgat acctatccat
     1801 cgaaaccatt tcatatcaaa acaccacttt catatccaac gtgtcttcgc cgcgtttgat
     1861 ttttacgttg gttgtttcgc cttttgtaaa ttttgacaac gcctgcatgt atgtttgaat
     1921 atcaaaaacg atgtgctctc cgatttgaac gatgacatca ccggctttta gacctgctac
     1981 agccgccggt tttccgtccg tgacaccatc cacccgcatg ccgccggtaa acgcgtaatc
     2041 cggtaaaata ccaagcgtta ctttaaaccg gggcgaatcg cttgcggtag aattattgcg
     2101 cgtcgccgta aaggccattt tgggcatggc ttcggctttt tcgatgatgc gctggatata
     2161 ttcgagtacg gccttttgac cgtcataatt gactttgtcg gcatcatccg tcggcttgtg
     2221 ataatcagga tgtgagcccg tgaaaaaatg cagtacggga atatttttca aataaaaact
     2281 agtgtgatcc gaaggtccga tgccgctgga atccgttttg atctgaaaat catgtgatgt
     2341 attacgaaca agattttcta atgccgggct tgttcccgtg ccatatatca tgagacgtcg
     2401 agccgaatcc aatcgtccta tcatatccat attgatcatc aggtggattt tattgaggtc
     2461 aatgctcgga ttggccgtaa acgcctttga tcccaataaa ccaagctctt cgccggagaa
     2521 ggcaataaat aaaaaattat atttttcttt aattttgttc tgcctgtaaa agcgagccaa
     2581 ttcaagcaac cctgccgtcc ctgatgcatt gtcatcagcg ccgttgtgaa tttttccttc
     2641 cggattggga tcaagagacc ctccttgccg acctaatcca aggtgatcat aatgcgcgcc
     2701 gataatgatt gtattttcgg cgccgttatc caaataagca accacgttat acgcggttcg
     2761 ggccggttgc gcagtatcta ttgccgcatg cgggttgttt ccgttacgcg gtcgaaattc
     2821 aaatttttga agaaaccctt tatcgttacc gtaagcagct aagccgtagc gtttaaattc
     2881 cgaagctata taaccccgcg cttccgcttc acccggttca ccggttccgc gaccttccat
     2941 ttcatcaccg ctgagttttt ggacatgccg tctcagtata gatgcgtcga tttgtgcata
     3001 taccggttta atcaaaaaaa acaatgttga agcgatgaga ctgaggcgta aaattttgtt
     3061 acggatgtgg cgtttcatga tcaatcctcc caatcggcga taaataaatt agtatctcgt
     3121 gttccgttat tattacgatt ggacgaaaaa atcaagcgtt tcccgtccgg cgaaaacatc
     3181 ggaaacgcgt taaagatgct ttcgtgcgta atgcgctcca gtccgttgcc gtcaagattg
     3241 atcatataaa gttgaaaatc atagccgcgt tgggagtgat gattggatga aaaaattatt
     3301 ttttttccgg acggatgaaa aaacggcgcc cagttggcct taccaagttg ggtgatctgc
     3361 cgcagatccg aaccatctac attgcatgta tagatttcca tattgctcgg tgcaaccaaa
     3421 ccttggctca aaagctcttt gtactctttg atttcatctt cagttttggg acgtgaagca
     3481 cggaaaacta attttttccc gtccggcgaa aaaaaagcgc cgccgtcgta accaagatcg
     3541 tgggtgactt gtttgagctg cgaaccgtcg gtattcatag tccaaagttc gaggtcaccg
     3601 gaacgcgtac tagtaaaaac aatcttatca ccttgcggtg aaaccgttgc ttcggcatca
     3661 taacccggcg tttgcgtcaa tggttgtaaa atacgcccct cggtatcggt tacaaaaatg
     3721 tcatacgtat caaaaatctg ccacaaatat ttgcgatcac tacgggaggg cggcgggggc
     3781 gggcaatcct ttccgccaag gtgcgtcgaa gcatagacta tgcgcgtatc gcctttaaga
     3841 aaataagaac acgtcgtgcg acctaatcct gtgctcaact gttttggttt gtacgtagag
     3901 tcggcgctta caatatccat cataaaaatc tgatcacagg ataagcccca tgccggatta
     3961 ttgctttgaa aagtcaactt ctttccatca aaactaaaat acgcttccgc attgtctccg
     4021 ccaaacgtca attgacgcaa gtttttcaaa tgcttctccg atgacggcgt ttgcgccatc
     4081 actacacccg tcaccgtcaa aagaacccat cgcaacagtg cactcatttt ttttcatccc
     4141 tgtttcatat aaagaaaaaa ggcacgtgac gacaaaagag tcatcgtgcc tttttttaaa
     4201 accatatttt attaatggaa ccagctattc acaaaaccga aatcagcttt aaaattgagc
     4261 gttaactcat ctccggcttc cacagtaatt gccgccggtg cagggtagtc gtaacccggg
     4321 aagtaaggca gtgccggagc aatgtatata ccatggctta cgctatccgg cattccccaa
     4381 tgcactccga gtgttgcggt ttttttgtcg gaggacactg cgcggttagt acgaaatcct
     4441 acggccagcg ccgagtatgt accgggttca acttcaattt cgtattgata tgtcgagtca
     4501 ccggccgcac gggtaaagac caccggattg ttggcattat atggcgcacc ataaggcacg
     4561 cgaccgcctg ggccgttctt ccaaacggaa tcagcaaaaa ttgtaacttc tacctcacca
     4621 ctgtcttcgt cagctttggt aaaactaccg gatgctttcc atacgcttac atttttgaag
     4681 gttacggttc ctttgatggt agccgttgtc ggctttttgt catcatcaca gccagataat
     4741 atcatgattc ctatggcaag gatgaggcta agtaataatg tttttttcat aaaattgcct
     4801 tgtataatgg tagtaggttt aatgacactt gcaatataga gccatagaat accacttgtc
     4861 aatgcggtga gctacctttt acaaatatat tacaaacaat gagatatgat taaaattttt
     4921 aaactatatg actaaaatca tggtattttt taatcccttt tgttgtgatt ttttgtatga
     4981 aatctttaca ttacatttaa accttggtac tgcgatcccg tctaatagaa caccaggaga
     5041 agagaatacc gacgcatgac ggatcaggaa atcatagcaa tatttcaatc agccgaaggc
     5101 gcgaaacgtg atcaggtttt cggcgacatc gtgcatcggt atcgagagag actgtatgcg
     5161 atcatcatgg gttttaccaa tgatcatgac gaaacagatg acttgcttca ggaaacattt
     5221 atcaaggcgt atcagcactt gcatacattt cgcgccgaaa gttctttgta cacatggctt
     5281 tgtcgcatcg caatcaatct ttcgatttca tatgcgcgca aaagaaaatt gcgtcagttt
     5341 ttcagtgtag atgcgatgga agaaatttta ccatccggac aagatgggcc ggacgcaaca
     5401 acggaaaaaa tggaactgcg aaaacatatc aaaagagccg ttgaccagtt atcggaaaaa
     5461 caaaaaaagg tttttatttt gcgctattat gatgaactca gccatgctga aattgcacag
     5521 ataaccggaa attccgaagg aacaatcaaa gccaattttt ttcatgcgat gaataaagta
     5581 caggcgtatt tagaaaaaca ttattttgaa aaaggaaaac cccatgcgtg acgaatgggt
     5641 agcctaccac aaaaacaaat tgtcgccgga agcgcgacgc cggtttgacg aaaacctaat
     5701 ttccgatccc tctgaaaacg aggcctttca gaaattcagt cgcgttgcgg aagctttaga
     5761 atcggctcgt ccggacacgc ctgaaggata ttggcgaaac ttcaacgtac acctgatgga
     5821 caggatcgcc gaaaagcgta aaccacgttt ttctttttca tggaaacttg ccattcctgc
     5881 gatagcgaca gccgctttgg ctgttttttt cctcgttccg cataacacag acgacacaac
     5941 ggctgtgcat ccggacgtgt ggaatgattt agcgtcttca ctcgaatcca tggaaacgtc
     6001 ctccgcgttc gagtcgggcg ccgatataca gatcgcatcc gaagatgtcg aagaaacaat
     6061 ggtgacagcc gacgaatggc atgatttttc aagtgctctg gaatctattc gtttttcagt
     6121 caatgaaacc aacgccgacg gcacggtttg ggataaaagc gccaaagaag aattactcaa
     6181 aacactcgat caaacatcta taatctaata attcaatcat aggtatgcgc atgaaacttt
     6241 ttacgatcct tggtgtaact ctgattctcg ggacttcagg tgtatctctg cacgcgcagg
     6301 aagacgctga gttcaaagaa cggatcatcg aaatcaaaaa gaagaaactg ctcgaatccc
     6361 ttgcactttc taaagacaag gaaaaccagt ttctcaaagt gtatgacgaa tatttcgata
     6421 agcgccgcga cctcaatcgc gaacgtaagg ccgttatgaa acgtatcgct catatgtcac
     6481 agcttcaggc ggatgacatt cagggcgata aagtgaccaa agctatcgaa gagctcggtt
     6541 atgtcgaacg taaaatcgta gatcaacgtc aacgcaccgt cgaacagctg cgcggcactc
     6601 tgactgaacc cgaattagca aaatttgtga ttttcgaaca gaatttccca atccgcatgc
     6661 gggaaatgat ctttgatatc cgcgcccgga aacataaaga caaggacatg cgcatgttta
     6721 taccgccggg cgaggacgat ttattggatt aaatgaggta ggtcacaact tatattttgg
     6781 caggataata tcgagacaca aaaattacct tcgcacactt aactgaagga aatcgaagcg
     6841 tagatgtaaa cacaacatca atctacaatt caccgtttta caccaaaagc aaaatatcgg
     6901 gataagtata aaactgtaat ctaaaactta tttcttgtcg ttatgacaat cgtaacaact
     6961 catttcctgc cacgctttat ccacattaac cggatggcgg taattcaaac cctgcattga
     7021 aacctcttgt ttgtcatttt caaacttctg cgcaagcaac gtgtggcaaa cattacaatc
     7081 ccgtgaaatc actttaccat catcgctttt gtgtttgccg tcatgacaac ggaaacatcc
     7141 tgcggcatac atatgtccta tattatccgg ataaccacgc cagtcagcac gcatttcagg
     7201 aaaataatta cgactgtata ttttttgcgt ttcctgtatc gctttatcca ccttagcgga
     7261 ttgatgttta gaaatatcag gataattttg actgtaaaaa tcccgtatca caatcgcgat
     7321 gctgtccatc gctcgggatg tagatgtata aggtatctcc aacgctttga tagaaatgtt
     7381 tttggcatac ggcaatgacg gatcaatcca accgagctgc atatattgat tgacctgatt
     7441 agccggatgg ttgtatttat ggcttggacg attgtggcaa tctatacaat ccatgcggcg
     7501 cgtttcatgg tttttcaact cagcttctga aattttgttc tccgtggatt gatatacagt
     7561 ttctttcccc gtttttttat cgcgaatacg gacccatgga atttcctgac gtttgtgatc
     7621 ggtagagaca tagtccactt cattggcaat attcatgtgc cagtgaatgc cgtcggtgtt
     7681 accggacagt tcattgccac cgccgatgcg cataagtaaa ttcagcgtcc atttggtatt
     7741 gttctcatct gaaacgtaat acgtaagggc gcgttgcttt tcactgaaaa aatggcgggg
     7801 ccagtggcac tgttcgcatg tttgttgtgc gggacgcaga ttctcaatgg gcgtcggtat
     7861 cggtttagaa aatttattaa aaagcaccga atacacctga tatgcaccgg aaatttttgc
     7921 tttaacaaac cattccgcac cggagcctat gtggcactcg gcacatggga cgcgtgcatg
     7981 cggtgaatgc gcataggccg tatattcggg gtgcatgaca ttgtggcaaa cttcaccgca
     8041 aaactgtacc gaatccgaat attctaaaac ttccatactg ccaaacgccg ataaaccgag
     8101 caaaataatc gttccgaaaa aaaacacggc ggttgcacgg cgatgacgcg gatcattgag
     8161 gtctatgtgt ggtagatgtc cttcggaggg tgttccgcgc gcaatacgtt tcttttcgcg
     8221 ccatgcgcca tatgcaatca tcaataaacc gagtatcaga aacgccggcg ctaccatgaa
     8281 agtaaaaata cccatgtacg gattgctatg atccgccgta atttcgataa gaaaaaggaa
     8341 aataaagacg gcaaaactgg taaccgacaa tgcggcgcca ctcatactta ccatattata
     8401 gagtgacgga ggaaataatc gtctcatagt tcggctccgt cagtttttac tgttattaat
     8461 attattttcg tcgggcgtat catcggcttt agcatccgat acgttttcct gacttacacg
     8521 aacaacctca tcttgagggc ggttagcttc ttctgatttt ttgatagctt caagttccaa
     8581 cggatgttca tgttccattt ctttttcaga gattttaccc gtcaaccaag caaaactcat
     8641 cggatatacg tcaggattga agatgacaaa gtaaaaatgc catacgatga tcgccagtgt
     8701 agccaaaatc gcttcataaa agtgaatgat acgtgacaca tcccagccca acttggtgat
     8761 catgtcaatg gactgcactt caaaccaaag aattgcaccg gtcaaggcca tcacgatcgt
     8821 cccccacacc aaagcccaat attcgctctt ttcgacataa ctgaagcgac ccaagcgcgg
     8881 tttgtcttta cttataccaa ggttatattt gagaataccg acagcatcag cccaatcttt
     8941 tttacagaac cacagatctt tgaccaactg gcgtccgcgc accgtaaaaa tgatatacca
     9001 gagatggtac aaacttacga cgaccatcgc gatacctgca acacggtgca atatactgcg
     9061 tgcctcaaag gcatattcgc taagactacg gatgccaact acccaccatg catcgggata
     9121 ccgcagcata aagccggtaa tcacaagtat tatgaaactc aaagccatcg tgccgtgctg
     9181 aagtcgctca gatgccgtca tgcgaacata cagaccccga ccatgatgtt cttcttcatg
     9241 cagttctccg cgttgaatgg ccagcttacg gcgcacttta cgatggatat ctatgatatt
     9301 gtgcagagcc atgcctccga caacgacgaa aatcaaaatc agataaatcg tagccacata
     9361 ccaaagtaac ggatcatccg ctttcttttc ttcagatgaa tgcaccgacc cgattgtaaa
     9421 acgatcgccg gcaccagggt gacacttgcc gcaagttttg actaaattgg cttttgaaat
     9481 cgtcgaggtc gaatctttag cttgtttgat attatgtgca ccgtgacaac ttgcacagtt
     9541 gacgacttcc aatgccccgc cgcgtaccga tagaccgtga taactatcgg agaatgtttg
     9601 aaaacgatcc gaagcgatac catatttttt cgatagcttt accgatgcat gacaggtacc
     9661 gcacacttgc tgtgaaacat tgcgagacga taccggcgac ttaggatcat catgttttaa
     9721 aatatcgtgt tcaccgtgac agtccgtaca acccggcgca tccttactgc ctttgtgcaa
     9781 catatttcca tgaatactgc tattatagtc attggcaata tctttatgac atttcgcgca
     9841 tgtttcggga atatgcattt tgttgacggg cgagttatct acgatggcct tattcatcgt
     9901 gtgactgccg tggcaatcta cacagttggc cgcctccgct tttccatttt ttaaagcctg
     9961 accgtgtacg cttttttcaa aactgcgaac aaactgcgca ccgagcaatg ttttcgatga
    10021 aacttcctga ttatctacgt gacaactcat acatactttg gcttgcgcca ctttgagttg
    10081 taacgaatcc atgcccgctg cgatgtgcgt aatattgctg gagtgacatt ggatacaagt
    10141 cggcgcattt ttatcgccac gcttttgcgc ttcggcatga gcggaatgaa taaaatcttc
    10201 ttttacatcc ttgtggcacg aaccgcaacc attgaccaga ttatctttat gaaaaatatt
    10261 tcccggtact tttgtcgaag ccacgtcgtg tgtgccgtga catgttttac agttggcatc
    10321 atcgctccac cctgtacctt tggttgccat gatattgggg tgaaatgcat gtttggttaa
    10381 ttctttatta tggcaggaaa cacaattcac cggcgtgatt ttttctttgt gcggaatttc
    10441 ttccggatca aatccggtat ggcacgcgat acagttgact ttagcgtgtg ccgatttacc
    10501 tcggattttt tcatccacaa aaagcgaaat ctttttaccc gccttttttt tgctgagcga
    10561 ctcgtcggag tggcattcca tacaatcatc gttggactga gccttgagcg aaaagccagc
    10621 gaataaaaaa atcacgataa gaatggtgtt taaaatgcgg cgtgagaaaa ccataacatg
    10681 cctgctgtct ttatatttca gaatggattt ttatgattac caaactaccg ttttttataa
    10741 cggcagctgt ttttccagta caggctgttt tccgatttgt aaagttcaat acgtagtaaa
    10801 cggcctgttt tttattaaaa aaagaatctg gccaaaacgg gttcggccag attcttatat
    10861 cgcacatata tgaactgtac gggttattgt ttcgttttct gttcggccaa ccaagcggct
    10921 aatgtcttca gttcgtcttc cgtacctttg aactttttca tgtgtttttt gccgttgatg
    10981 gcttcaagtt tttgaagata cttggcaata aactcagcat ctttttcaag accgacgccg
    11041 gaaagatcag gagctttgtt ttcctccgct tcttcacctt cggctttttt agcttcgata
    11101 cctatgtttt tgaccgcatg gcacgacata catttattgg cttcaaaaat agcctttccg
    11161 tccgcaggtt tggcctcttg tgcattgatt tgcgctacat ttacaaacat tactaataaa
    11221 ccaaaagcgg cgataaacat gagttttttc atagtattgc aaactcctct taaatgtttt
    11281 tctatcaatt aaatatacat tagtttcatt gttcacagtc atactatgta aaaattatgt
    11341 aaagaaacat gattttcgtc atcttgataa tttttggtat atctcgtaag tgcttaaaaa
    11401 taaatcggct acagcgaatc tgtagccgga taggatggct aaatgtatgc aaataatgaa
    11461 acagtaaccg attattcttt cgtttttaac tcagcaagcc acgacgccaa cattttaagt
    11521 tcctcttcag tacctttaaa ttttttcatg tgttttttac cttcgatcgc ttcgagcttc
    11581 tgaaggtatt ttactataaa atcagcatct cgttttaacc ctacaccgga tagatcggaa
    11641 cctttgttgt ctccggcgtc ttcgccttcg gcttttttag cttcgatggc catggatttg
    11701 atagcatgac atgattcaca tttaaaggac agaaataccg ctttaccgtc cgccggttta
    11761 gcttcttgcg cataagcctt ggaagacaac ataagaatcg ttgcaaaaaa agcgatcgcg
    11821 aaagcaacta tacttttcat aacactcctt gataagtatt gattaatccg ccacacatgt
    11881 cctatgcacg cgacgcccgt gcggcgtagt gagaataatt cattttattg tgtcgtgcga
    11941 agtctgaaaa taaatggata cgaaatccac actttgaccg ggcgattacc attgattgcc
    12001 ggtttgaaac gcgcctgcga caaagcattc accgcggctt ctttaaacat atcatcgccc
    12061 tgaatgtaaa caactttgac aacgactcct tcttcgttaa ccaatgcttt caaaaaaact
    12121 ttaccttcaa taccggcctg acgagcaatt tcaggatatt cgggtattac tttagccaat
    12181 aatatcggcg cttgttcgac cgggataaat tcgtccattc cgggttcatc ggaactttca
    12241 ttgagaccgc gaagtgattc actaatatct acttttattt cgccttcgcc ggcattaccc
    12301 agcgaaaagg tcgaagcggc gatattggtc ttaatttctt cctgagtttg tagtgttaca
    12361 tccgatggag cttccgcatc tggcacgacg atgggaattc ccgctattac ctttggtatt
    12421 tcgttcattt gaacatccac cttgactggt tcggccgttt tttcaagcgg tgccgcgctt
    12481 gtaagctcat tggcatccac aaaacgaacc gtacgcatgg atatatcttc tttgagaaga
    12541 acagcgctta catagtatga agtaataagt gccgcgtgga acacaagcgc tattgaaaga
    12601 cccttccaca gattgatgct gctgatatgt attagaaaat tacggcccga gtccgggtga
    12661 agtccaaatc cttcaagact ttccaaattg atgactttta cattcatcat agagccgatc
    12721 acctcctgct acaaggtaaa acgcaaaagg catagagaaa atgcccgacg tcatcagctg
    12781 ttttgactta tgtcaaagaa taggccttaa taataaagat gttacaaaat taccgcataa
    12841 cgctacggca gtatcaacct ctcaaggttc tcccccacac gacccattgg ggttcgaacg
    12901 actacccacg ccggaggcat atccaaagcc cgtttaatca cgatcaccag acaaggcaca
    12961 tttcgcttcc agttccgaca ctaatgaaaa cctcggagcc nnnnnnnnnn nnnnnnnnnn
    13021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13081 nnnnnnnnnn nnnnnnnnnn ggcacatttc gcttccagtt ccgacactaa tgaaaacctc
    13141 ggagcctagg ggcaccacgt tttgcccgca acagatagtg agcttagtcg agctaagggc
    13201 caaagaggta tcggttaaat ccctcaagaa cggactggaa agagtcagtc gcatatgtcc
    13261 aacgtgtccg cagaataagc cattctttca aacgccacat caatatagct aacgtcttcc
    13321 gaatctccgg catagtatat gggataggtg acgatcagga gtgtcgaccc gaagctgaga
    13381 cgataagcat attccttgtc gttggatata agtttgccgt cccgcacttc cagagaatcc
    13441 aagacatagc aggaatcttt tggccatgca aattgtccgc cgccacccgc ctcatcgaaa
    13501 gcgtagtagt gtctgtttcc ggggaaagtc aaaagcctgt ttctccactg cgtgtttgca
    13561 agcctcttat ccggcccgga atcattctga tctgaattgc atgcagtgaa tgcaaaaatc
    13621 agaataagca atggcagttt caattgcctt gtcatgatgt gtcctgtaat aatcttcaaa
    13681 gtttttccaa catgccacct ggctttagcc aaactaaaac aaggaagcga ttcatttgag
    13741 tacactcatc tcagtggttt aagtttcatt atggcattat cattgttgtt caacatatag
    13801 aaacaatcat cttgcagaga tccaaatcga aaaaaagtct cgtgattctt tatgctctgg
    13861 tagagtaact gtccattcaa tgcgtctcgg gcttcaacaa agccaacgga tcccatgttg
    13921 taaacgactc cattgaacaa ttctggctcc cattcagcag attccccaat tcgagagttg
    13981 gatcgccata cgagatttcc agagtccaaa gtaagacaat aaaactcaat tcctgcgacg
    14041 acgaatccgc gttgattcgc atcgtcaacc gtcatgccag caaaagaacc gctttggagg
    14101 tctttaatcc aaatttcagt tcccgtgaaa cggtttagac agaacaccga tccgctggaa
    14161 ttaacgatga aaacacgatc ctgccagatt gccgctcggc aatcggctgc accgctcacg
    14221 aatccggcgt tcgggcgatt ggaaacatcg taactccaga tcaaatcgcc ggtttgcgag
    14281 ttaaagcaat agaccaagcc gtatggcacc tccccgcgct cgggatgcat acgaaatccc
    14341 tgagtggtaa cataaacata tccgtccgtt gaagcggcaa tattgttcac accttcggag
    14401 agtctcctct tccagttcaa ttggccagaa cccctgagca cacaaaagat attaccttca
    14461 ttatatccag acggtgtgta cgaatctccg gtaaatacat actcctccga aggttcagga
    14521 tctgagaggg ttccgtaagt ttgtgatttt acgaattgcc agtcgatcgc tccactttcc
    14581 ccgtcaaaac agataagccg attggagtaa tcaaagtaaa tgtgacctcc gtacgccctg
    14641 aaacgattaa cgatgggctg tgaagtctga ccaacttttg tggcccattt ttttgcgccg
    14701 ctctgaatgg aaaaaccgtg aacttctcct gttgtcgtcg cgaaggtgac tgttgaagtg
    14761 gttgctgcgg gaatagtgta tgagtacaaa ccctgagtta ctttgacttt ccaaacctct
    14821 ttcaggtcaa ggagttgatc acccccttca tccaggcaac cgccacaaga acacaaaata
    14881 caaatcaaaa tcaaatatga atgtttaagc atcatttttt gtgataattt aagggatgag
    14941 taataatacc catcccttga taataccttg aacttaatac caactgatcg tgtaactagc
    15001 cacttggcct gaaacattat cggttgcgtc tgccctcaat tgatgttttg agctcggttg
    15061 gaaaaccttt gtaggcccgg ttccgatata tgtccaggtg ccaccgccgg gtggaacaaa
    15121 cccttcttta agcatcgcaa tctctccgcc tgttccacca ccttcattgt agtaggaata
    15181 ccatttaact gtatagttgc ccgatccatt ggccacattt gctgtaaatg tataactccc
    15241 gccggccggc gcttgtgatg gttgcggtgt gacagaaact gtgatcggcg caggtggagg
    15301 cgtcatgtga acgaatccga cgtcattcaa gcccagacta atcgcatctt ttccgcgatt
    15361 gcttctcccc tccaacagat ggtttgtgtt gtagatgatt cttggcgatc ggtttgcatt
    15421 tgggtagtcc tgcgaatagt caggaacgac tccgtcgctg tatgtccaac cataatcgcc
    15481 attcgaataa ataatactgc cgttgactat tttccagtga tagcccgcgt aggcaatatc
    15541 caccgcggag gcatagaata tgtcgccaat ggcccaggcc gtataagcat cggaaatgta
    15601 gccctcatcc caaagccaat ctccgtattc ccagtaaata taagcaacaa tcgcgtacac
    15661 agcgtataaa aaatcttgaa tatcggctcc cagttgcgga tcattggcta gttgcgtaaa
    15721 ggcaagacga taaggttcgt gcgccggatc gagatcaaga ccgatgattc cgacgcggtt
    15781 gaatgtctcg atattatttg aattcaagta ttggataccg gcgctcccgg gccttaagtc
    15841 attcatagca ggactcgcta gaaacatatt catcagatcg gcaccgacta cgatatccct
    15901 aatgctccac aaagcttctg cccagtcagg gcctaggacg taagccaccg gactcaagat
    15961 ggcattccct tgaactgcaa cgtagtacga taagttgccg ttgtacgtgc tttccgctat
    16021 gaaagcgcct tcgtgaggtg ttcctaatgt cacaatacga ttgatctttt ttgcagactg
    16081 ttcttttttc tagtaggtgc gtgcggcaag accacccatg ctatgaccca cagcgatata
    16141 gttatttatg ttcgggttca ggctggtctt tacgtcatcg gcttgaacac taaatgttgt
    16201 ttgatcatcg cgtgtcggat tggtggcatt catgtagaac gactgtttga gttttgcaat
    16261 catgttataa ctaccagtcc acatgccgcc actggaattg atgccatgca agaacacaac
    16321 attttcctgc gcgcgaaggc ccgtagcgaa aagaaaacat ataaatatat ggttaatcgt
    16381 tttcatttta gcctcgttgt ttagttgttg gtgatgctaa cattggatac agtttcggtg
    16441 taagtccgcg tccgctgttt accgcccggc aatttttggg tcttagtaac cttggtctta
    16501 gatctatgct tacgcccctg gctctcagcg aattcgtagc tcttctgtag aaacagctcc
    16561 ccatccctgt aaaccttttc actggtgatg gtcttcaatc tatcatcgta tgccagttcg
    16621 atcttaccga aaggccgatc aatcgtaaac ttccattcat ttcccgcaac tttttctacg
    16681 gcatcaaact gctctcgggc cgtcgtaaga agatcttttg gttcaacaaa gatatttgcc
    16741 ggcttcgaac aacgcttcga agaaacggat tgttgttgta cgtcacccgc cggattcagt
    16801 gacagcgact gcggcaaata tgggtttcca ggaatcagaa ctccatttcg gttatacatt
    16861 ctcacggttg atccgtcgta cacgatgctt gacgggcgtc ctagagccag aagagaatcc
    16921 gccgcacccg tgagcggtgc aaaggtaata gtcatcgacg tcgtaattcc gtcggtctgg
    16981 gtcaaggaaa cgttttgcga ataggttacg gtatggtttg gccccaaacc cgcggctgta
    17041 tttccgctga cgtcgggaac cgcgtaaggc ataaaggtat catcttcttc gacctctgtt
    17101 gtcgcggtat agctgatcgt tgttgttgcg gtcgtttttt ttggcgcatt gatttcgggc
    17161 gctatcggac cattcttatc gctcgcgtcg tcgcacgaga ccatcaaaaa cattgaagct
    17221 acgattagga tattgttctt catttctttc tcccattttt tcgttttatc aaatgaattc
    17281 cccgatactt gcgacggaga ggttcatttt ctgcaatcgt ggattatagc atcggggcag
    17341 ctatacatcg gtcatcactt cgtaagtgag gatgattgat aggtcatgcc tgcttccaaa
    17401 cgtacattcc atttccattc aaaatacttt acaatcacaa tactttggcg gttccatgct
    17461 ctcacctcca attcgtaccg gtgccgtacc ttgagattcg gtccgattct caaaatcgtt
    17521 cgtaattaaa gtagataata aaattgggga atccctagtg aactttaggg agagagaaac
    17581 tatttgtcaa gagtttttcg attttttgaa gattttgaaa tgcagattac gcgcggctcc
    17641 ggatgaactg tgaatttaat cctccgaccc cctccctcca actacccaat tctattagta
    17701 tttgaacgag tgtgagtgag gttgtgtaag aaacattctt tagtcgcacc ttctgtgtga
    17761 aactcgtgtg gtcaccaaag cgggagaaac aaaacgccgt tgcgaatttc atatccagat
    17821 gattgatgtt ttcatcgtgc tcaaagacat aatcttgggc ttcaagaatc tgctccgagt
    17881 cggacgatac agatcccatg gaaaactccc gccggcacac aaataacttc aatcggaaat
    17941 tcgacttgaa tcagaagcag accttataag gaattgaaag aatgatctca tcttggagga
    18001 tgctgattcg gattgcggtc ttcaagatca ttcccgatag tcccgaccga gtcaggataa
    18061 aacggtaccg agttcttttt taggttgttc gataggaagt gtgaaaataa attcggtacc
    18121 tttaccggga ctggtgtcca cgtcgatttt tccgttgtgt ttacccataa tctcgtaggc
    18181 taacgcaaga cccaaccctg taccttgacc gacgggtttc gtcgtaaaaa acggatcaaa
    18241 tatttttttc ttcacttctt cgctcattcc catgcccgta tctttgatgc gcacacgaat
    18301 atttttttca tccgccattt ctgtcgttat cgtgatcgta ccctgacccg gtgtcgcttg
    18361 tacggcattg acgatcgtat tcataaaaac ctgattgagt tgaccgggaa aacactcgat
    18421 ctgaggtatt tcgccatagt tttttatgat ttcaatccgg cctttgtatt ggtttttagc
    18481 gataactaaa gcactattga tcagtttgtg cacatcggac atacggtaat ctttgtgatc
    18541 ggctcgggca aaattgagaa gaccttctac gatttcaccg atacgctcgg tagccagttc
    18601 gatcggcggc acaatctctt cttgtatatt ttcaagagcc gcacgcatat cattcggatt
    18661 ttgagattga accgcatctt gcaatttgcg atacaaaata aacacataat gatccaccgt
    18721 tgcccgcggc gtattgactt catgtgcaat accggccacc atctgaccta gggatgacat
    18781 tttttcggct tgaacaagtt gggactgcgt tttttgcaga tcttccagcg ttgtttcaag
    18841 ctgttcggtt ttttggcgaa gttgcgatgt gcggtcagct acttgttttt ctaaaagctg
    18901 cttctgatgc agcaacctcc gcatacgctg tttgtaccac gtcaccgctg aaaaaatcaa
    18961 cagcgctaag gccgcgatac gaaaccacca cgtctgccag aacggaggct gaataacaat
    19021 tttaagttca acgccttttt cattccacac cagatcgttg ttcgaccctt tgacgcgaaa
    19081 aacatacgta ccgggatcga cattggtata attagctttg cgctcggtac cgatattaac
    19141 ccatccgtta tcaaatcctt cgagacggta cgcatattga tttttttcag aactggtata
    19201 gttgagtgcg gcaaactcaa atgagaaaaa attctgttta tacgaaagct caacgaccgg
    19261 aagatcattg atatccttgc ccaacgctac cggtttttcg aaaattttga actgcgtcag
    19321 aaccacgtta ggaatattgg gattatcgct tacgctgtcg ggatgaaacc ggttaaaacc
    19381 ccggatacct ccaaaataca tttcaccgtt ttgcgcttta tacgccgaac cgtttctaaa
    19441 ttcgttactt tgtaacccgt cactgacatc atacacacga aacaccccgg atgaacccga
    19501 tgcatcagac ggtataaagc gtgtaagccc tttattcccg cttatccaaa gacgattttt
    19561 agcatcacaa agcaatccgt agataatatc gctgggtaac ccttcttttg tagtatagtg
    19621 ctgaaacgta gattgtatgc gatcaaaacg attgagtccg ccggtcgttc ctatccatag
    19681 cgtacctgaa gtgtcttccg taatggcgcg gatattatta tgactgatag tagacagatt
    19741 gcgcggatca tgcaaatagt gacgaaacgc cgtttcaccc ggcaaacgca cactcaaacc
    19801 gccttgggta cccacccaca atgcaccgct ccgatcctga taaagcactt tggccacatt
    19861 attacgtaaa gaagccggat catcaggatt gttttgataa tgaataaaac cgtttttttg
    19921 cggatcaaac agatcgagac cattcatcgt acctacccat atatgcccgt cgcgatcttc
    19981 gagtaatgta atgattcgat tttcgatcag cgacgcgggg tctacggaat gtttataaga
    20041 cgcgacaaac cggccgcttg cgcgatcaaa acgacgcaaa ccgctgccgt aacttccaac
    20101 ccataaatta cctttactgt cctccagaag acagttatag ctgttatccg cattattttt
    20161 gagtgacgca tccgcaaaat agtgattaaa cgtatttttt acgggatcta attgattgag
    20221 tccgtattcg gtagcaaccc atatttgttt tttgacatcc tcataaaacg accaaactac
    20281 attattgctt aaggatgttt tattcttagc atcgctcacg tagagggaaa atttttgctg
    20341 atgcggatca aaccgggaca cacctccgcc ataggtgcca aaccaaagac gctgggcatt
    20401 atcttcaaaa atcgtattga catagctgtg actaatactg cttttgtcgg tcggatcatt
    20461 gacaaaacgc ataaatcgtt tttcttcgcg gtcgtagcga ttaagcccgc tttgggtacc
    20521 tacccagatc acacccgcat gatcctcaaa gaccgaccat accatgttat gggaaagact
    20581 tgctggatcg gaaggattat tcgtaaacgt cacaaaacga tcggtaaccg ggtcatacat
    20641 attaagccct tcagccgtac caatccaaat cattccacca tggtcctcgc aaaggcttaa
    20701 tatgttttcg tgaatgattt ttcctgttcc gatcccgtat tcaaatttta ctgtttttcc
    20761 tgtcgccaat tcgtgcgata tcaaaccctt attcgttcct atccaaattc gacctttggt
    20821 atcaaaaagc atcgcattca cttggcgcga aggtagcgct ttgccaatgg gcgttgactt
    20881 atcctgatgg ctaaatatac ctgtagattt atcaagtata tccaaaccac cggctgtagc
    20941 cagccacaaa ttgcccgacg catcttcaac gatgaagttt acacgattac tattaatgga
    21001 gttcgagttt ttcgggtcat tccgataaat tacaaacgat tctttgtcgc gatcaaaacg
    21061 acacaggcct ccgtcagccg ttccgatcca tatcaagcca ctccgatctt tgaaaacctt
    21121 gagaatacgg ttatccgaga gtgacgccgt atccaaa
//
LOCUS       DAOQNQ010000023        20955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_869_length_20955_cov_5.905215, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000023 DAOQNQ010000000
VERSION     DAOQNQ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 20955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20955
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_869_length_20955_cov_5.905215"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /locus_tag="PK754_02925"
     CDS             <1..1280
                     /locus_tag="PK754_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNB08487.1"
                     /translation="LDRVGANFLKVTEQIKKDTGVTNKSVKSPEIRYAGVLGAGVMGG
                     GIAYLFSNYDIEVRLKDINHQAITLGLDSAKKVYERALKRRKLTKQTFSRKMSYITGA
                     LDYSGFRRCDLVVEAIVENMDVKKKVIGELSSHVSDSCIVASNTSSLSITEMAKAYKH
                     PDKFIGMHFFNPVHKMPLVEIIRGEKTSDATVAAIYALSKKLGKQPIVVKDGPGFLVN
                     RLLMPYLNEAGHLMEQGVTIELLDKTMLDFGMPMGPALLIDEVGIDVAYKVGHIFYDA
                     FGERVQPSRVVEKLHEAKRLGKKGKLGFYKHAGDKPQVDPEVYALIEVNQTSTIRSEE
                     MVERMTYTMINEAAMCLAEGIVSQPSDVEIGMIFGTGFAPFRGGLLRYADTEGLDKIV
                     AQLEKYAHTYGVRFTPCDYLVQLQKSGKKFYNE"
     gene            1310..1468
                     /locus_tag="PK754_02930"
     CDS             1310..1468
                     /locus_tag="PK754_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08488.1"
                     /translation="MLGDKLISKGVINQSQLDECLKEQQSSGKKLGEVIIAKGYATQA
                     QIDAALAG"
     gene            1556..3064
                     /locus_tag="PK754_02935"
     CDS             1556..3064
                     /locus_tag="PK754_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNB08489.1"
                     /translation="MDKDLIAIQEARDIARAAKAAQKEFAKFTQQKVDTLVKAMADAG
                     LAAADRLARMAADETRMGRYEDKIVKNQFGTRDVYEYIKDMKTVGVLRYDDKLKFYEI
                     GTPMGVVCAIIPTTNPTSTVMYKILISLKAGNAIAIAPHPRAAKCTYEAMRILRDVAE
                     SMGAPKGLIGCIEHPTLEGTQALMKCPETSVILATGGPGIVREAYSSGKPALGVGSGN
                     VPAFIEKTANVKKAVADIVAGKCFDYGLLCSSENSIVADAALRDQVITELKANRAYVC
                     NNEEKIKLQQLMFPKGKLNTQIIGLPAPKIAQMAGVAAPSDATVLVVPCEKVGKEEPL
                     SAEKLSPVLSLFWVNGWEQGCDRALEILNFGGIGHTMSIHSQDNDVIMKFGLEKPAFR
                     ICVNTVSTLGAVGYTTGLAPSMTLGPGTLGGSITTDNIMPYHLMNVKRLAFEIRPFKS
                     SLVHGNTESQAKDTVKKTAEIPSAPVSSSSKNDSLSEKEIDKIVEEFIRSRK"
     gene            3173..4465
                     /locus_tag="PK754_02940"
     CDS             3173..4465
                     /locus_tag="PK754_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNB08490.1"
                     /translation="MVIDILVILILVLLNGFFVAAEFAIVKVRSTQIDTIEQKNIRAR
                     IAKEVIMHLDAYLSATQLGITITSIALGWIGEPAISRMIEPIINYFGITNPQVIHALS
                     FGIGFSVITFLHITMGELGPKSIAIQYPKQTTLWIASPLHLFYVVFKPFIFILNKSAN
                     LMLRMVGIHPAGEHELSHSEEEIRMLIADGRKSGVIDATEYKLIENIFNFTETSVKEI
                     MIPRMDVFALDIEKPVVENLKSAIDSGYTRIPVYRESIDTLIGILYVKDLFKIDRDTI
                     TTASFETILRPAYFAPESISINRLMQDFLQQRFHMAIIIDEFGGTSGVVTLENIIEKI
                     VGQQIQDEYDDEKKEFEILSDGSYTVQAKMRIGDFNQQFQANIPEDDNYETLAGFLNN
                     VAGHIPNNGEEIRYQNLSFRITKKSPKLVQQVRFAKLT"
     gene            complement(4477..5385)
                     /locus_tag="PK754_02945"
     CDS             complement(4477..5385)
                     /locus_tag="PK754_02945"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNB08491.1"
                     /translation="MFGQRKIALALGGGGARGLAHIGVLKTLERHHIPMDIIVGSGIG
                     AVVGAMYAQTLHIAEVESRIKKFFLGDDFKKTGLHLFRRDMHPENFFGQIARAVGNEL
                     LIQLYADRMSLMKENRLGIVMDELLGDGLIENTRIQFAAVATNMITGKTEIFREGSIK
                     EAAKASSSIPGFLPPMQYEDKVLVDGAVAAQTPVDAAWEMGADYVIAVDTSRTLAGEA
                     PPVNMVDLLFRTHLITALQANALMCSRSTVHIRPEVGHHHWAAFEKMNELIAAGEKSV
                     DASINAILMALKKEKSRLSKHRLSKV"
     gene            complement(5389..6429)
                     /locus_tag="PK754_02950"
     CDS             complement(5389..6429)
                     /locus_tag="PK754_02950"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNB08492.1"
                     /translation="MPKDKGKANTEKVKLGIVGAGAISQVIHLPILSKMNDVEIVAIA
                     DSDKTKVGALAEKYHIPNVFTDFEKLLAMDEIQGVHICTPNSMHAPMAKSALSAGKHV
                     LVEKPIALTYEESKTMVEESKKRKKILMVGMNHRFRPDAMVLKNFVKGKELGKIFYTK
                     SGWLRRRGSWNETDWAKKKNVSGGGVFMDLGIPMLDLSIWLMDNPKVHSVFSTMYSHF
                     QKDGVEDSAAVMIRFENDVVLSIEVSWTLLMENDFMYSNLFGTSGGALLNPLRIHKEM
                     HGNLVNVTPQKMEKPENNYKKSYEYELKHFVECIKNNRPSMSSGEEAMEIMRISEAIY
                     QSAKEKREIVLK"
     gene            complement(6451..7758)
                     /gene="aroA"
                     /locus_tag="PK754_02955"
     CDS             complement(6451..7758)
                     /gene="aroA"
                     /locus_tag="PK754_02955"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNB08493.1"
                     /translation="MATALIKPATKIRIDCRIPGDKSITHRAVIFSAMSESIVSLFNI
                     ATGADVQSTIDCMRRLGVKVDQNGSELHITGCGMHGFVAPSAPLYCGNSGTTARLLAG
                     ILSGQSFKSELSGDASLSRRPMRRIIEPLTQMGAKIKTSAHQTLPIEIEGTELKAIDY
                     SLPIASAQVKSCILLAGHYAKGITTVRESVMTRDHTERLLPTIKKSVEDGRISLSVTG
                     GESFRFDHMTIPGDLSSAAFLIATGLLLPESEVTIRHVSLNPTRIAFLEILKKMGADI
                     TIHNITEAREPYGDIVTRHTRELKSVQLSGSVVAALIDEIPILAVLGTHLKDGLEVRD
                     ARELRNKECDRIEAIVKNMRSMGIRIDEFDDGFRIYPGSFRSSEIDSFDDHRIAMAFT
                     VAALIAEGGESIVHSAEAVRISFPEFYSTLNISLSSDRMAVHQ"
     gene            complement(7828..9276)
                     /locus_tag="PK754_02960"
     CDS             complement(7828..9276)
                     /locus_tag="PK754_02960"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HNB08494.1"
                     /translation="MPVFVLVKDAHLYPTSILSVRTIITKGANSDELIFQVQEEIRQH
                     WWWKKWDIAVNESWIGHTVQLRIVVEYRTAGVTHTVEADNIIGTTKAPFDVYVSGDHW
                     PQPQGWFNGDLHVHSDYTEDAIEYGAPLPMLRAASEALGLNFIAVTDHSYDLDNKKED
                     YNQSDPTLAQWRTFIESVKQMNEESSSKVLLLAGEELSCMNIADENIHALILNSDHFF
                     YGSGDGGKNPLNTSSEHTVSGMARLLNKEALIMAAHPLEKPSPMERAFLQRGIWRLED
                     FYNRSAEVLQILNGSNDTAFKQGLGLWVHLLLSGRRVYIVAGNDAHGNFNRNRQVRIP
                     FWTIEDISHHKQLGWARTVLYLGEKKPNTQNTIDAIREGCAIISTGPYVVFNVLNEAG
                     FSSRIGQNAKGRRFRVAWEASSTQEFGALETISVFQGVIGESHEKVSTMTVESWCDQG
                     EFHTTADAPSYIRLSVTTKSQNFCYTNPIWLE"
     gene            complement(9475..9900)
                     /locus_tag="PK754_02965"
     CDS             complement(9475..9900)
                     /locus_tag="PK754_02965"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB08495.1"
                     /translation="MSSVTTNSFNMAIPSRTDNLGIIREFITSIARRFGFNDDAIGEI
                     ELAVDEACANVIKHAYKYDESKNIDITVETNGSKFTIIISDEGEGFDPDRLETPQQRL
                     QKHARGGLGIALIKRMMDEVVFDIHPGEKNRVKMIKYVS"
     gene            complement(9918..10274)
                     /locus_tag="PK754_02970"
     CDS             complement(9918..10274)
                     /locus_tag="PK754_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNB08496.1"
                     /translation="MNGFEVTRKDEQDITVLYLKGYLDAHTYPHFEGELQKLMEEKRY
                     RIVVDFQELSYISSAGLGVFMGFIETVRENQGDIKLCTMSTKVYKVFDLLGFPTIYQI
                     LKDPSEARDHFVQANK"
     gene            complement(10364..12640)
                     /locus_tag="PK754_02975"
     CDS             complement(10364..12640)
                     /locus_tag="PK754_02975"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNB08497.1"
                     /translation="MDPKNKLFRVALIFIPILFVIGVILNSFMFYAKIRFSAGDWGSE
                     LLSIAIIGLVLYVAFNYPRFESISITQKLRLSLFFVVTIYGVGLLMTLVTQPVFFTPR
                     YSGSYVLRAYTWSGVIVAHVIGFVAVLSLGSCLRILQTLIFYRRKKNTATFFHLFLLF
                     GALTALSTAITGRPLKYDPSSNHIITFNLLVITTIFLLINAGRVTWVTVLNRRQKFLT
                     FIGGLVFTLISAGLLGAEIGGGATYADMVYSYSRTAGSFLFLMTVFVFVYSISTTINT
                     LLHLPTAAIYDKKVREINSIYSLSRTINSLFDVEKIVISVTELVCDATGANACWLELA
                     NTKNPAFRTHFDFAAVRQREPFQVHFLEVSFRKFLSKHENARLIPSNDQHHHYYLTEP
                     TDWIINNKQPILINQVKRDKRTKDLKRTPIESLVGVPLITSDGMLGIIFAVKSTSFGF
                     DQDDIAIISALANQTTVAIENTRLVKESLEKERLAEELRIAHDVQMKLIPQEIPDIRH
                     DVSKMTLDIGATTVPANEVGGDYYDFVRLADWRCGIAVADVSGKGTSAAFYMAEIKGI
                     IQALAGIYSAPKDLLIAVNDVLFGTIDRKSFITLLYADVDISKSELRFARAGHCPLLH
                     MRDGVKTYVQPNGIGLGLDKGNLFRQAIEEKTIRIDDRDVFVMYSDGLVEARNAKDEE
                     YGEERLSKCLDGVEHLPAGVIKDKIIADVKRFVGSAKTHDDLTCVVFRLADLQKQVVP
                     VAPVTEAETGEITAVRRV"
     gene            complement(12752..13729)
                     /locus_tag="PK754_02980"
     CDS             complement(12752..13729)
                     /locus_tag="PK754_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HNB08498.1"
                     /translation="MKAILAEREKNTSRLVISEVDEPMVHPNDVLIAIKATALNRADL
                     LQRRGLYPPPAGVPDILGLECAGLILSVGSNVTQWSPGQRVCALLPGCGYAEKAVVHE
                     NMIIPIPEKLSFAQAAAIPEAWFTAYLNLVTIAQLKAGEAILIHSGASGVGTAAIQLA
                     KNLNAKVIASVGNSEKMRFCYELGADFVFDYHAKDFWNLIKTRCGGVNVVLDTVGGEN
                     MSHHVEVANNKARIVVIGLLGGTSSNLPTGTLLTKNISIIGSTLRNKSHEEKSDLTKS
                     MRELIMPHFTSGLFRPIIDSEYSWFDAEAAHMRMKSNLNIGKIILRIGV"
     gene            13919..14680
                     /locus_tag="PK754_02985"
     CDS             13919..14680
                     /locus_tag="PK754_02985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB08499.1"
                     /translation="MNVGTQHHVSDKKLSASSKSGVVWPALTEREIIRIAVEDTTRYE
                     PAMSEIWERYKKRLYLYIKYKLNINKGFETDKERYIIEDILQNTFCDVLENLNEYNPQ
                     YEVSTWIYNICNKHIVRYIREVQKYQGRTIAIEDESVEVIRLSSDSVKPDQEFEVKEF
                     ERIITFFVKSLQHKADQEVFLLYLQNLHNHNISDLLGKTQDAVRARLNRVIKRFKKFM
                     TKQYPEYLNSTMLSQIKNLSFGSANTENDFSPERG"
     gene            14695..15768
                     /locus_tag="PK754_02990"
     CDS             14695..15768
                     /locus_tag="PK754_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08500.1"
                     /translation="MKQLQNKIEVTADELFEQFVRELENYSTNGVVRSFETDRLWPLA
                     QHYIAQCAPMQERMDLEERIRLLLASKYQYTLQESRLKLSENLEQSIKEKIRALVRSN
                     IENAGKFAALAFNWTVIKPLQFAKDILQPVATNAMLTYAVAIQPSFTPAMDAIQLESK
                     KQISHIEVADEIKTDFLRVYKLPSVKTYSNEDLNAMNAGANTLADEKAPIIQKNVNKT
                     YHITPVQSSIKETSKLTIDVERARLIPESIPSVASSPVSYDRTRRELRSIGEVQQTIG
                     LHENKIFSCYQSYRKTSNRKNGRVAVKFLVSSKGVIKDVQVTANSFNLQMADRIVVQL
                     KAIKFSEVESRLGDQTIYHTFYF"
     gene            15821..16099
                     /locus_tag="PK754_02995"
     CDS             15821..16099
                     /locus_tag="PK754_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08501.1"
                     /translation="MLLKYGLIYMPASINIIAKRIGISETDRNLEVLKKELDKLVSKK
                     KAYRSGNRYAIDAEGARAISEYQDPKKLQEKPNPENFKKIFKQYSVAR"
     gene            complement(16139..16501)
                     /locus_tag="PK754_03000"
     CDS             complement(16139..16501)
                     /locus_tag="PK754_03000"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HNB08502.1"
                     /translation="MVIVDSKTGKTTFSVHLSEAESVNLLGNFNGWNEDKHPLKKDKS
                     GNWSITLKLTTGEYEFLYRVNKKAWMVDDKAPRKTNPFGTENCLVSVVIDMAKRANTE
                     KKSAVRTNKAKSSKTKKA"
     gene            16724..17290
                     /gene="efp"
                     /locus_tag="PK754_03005"
     CDS             16724..17290
                     /gene="efp"
                     /locus_tag="PK754_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNB08503.1"
                     /translation="MAGVADFRTGMTILMDGNLFVVTEFQHRNPGNWRAFVVAKLKNV
                     RTGRVIEKTFRSHDKLEEVRAESKNMAYLYSDGDMMYFMDNESFEQVGIPRESVGEAA
                     NYLKEGIAPAILFYEGNAVSIEMPITVELKITSTVPGVKGDTVTGGTKPATLESGAVV
                     NVPLFINEGDTVKVDTRSGDYLERVNTK"
     gene            17320..17847
                     /gene="accB"
                     /locus_tag="PK754_03010"
     CDS             17320..17847
                     /gene="accB"
                     /locus_tag="PK754_03010"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNB08504.1"
                     /translation="MDLKDLKNLIKMVDESNVNEVEIEQDNAKTKGRLKIRISKSGTT
                     HAMAVVQHSAPMAMPHHVPTQHLPPPAVQFESQETIEKSKSERPAGAVEQGNFIEVKS
                     PIVGTFYRSPAPDADVYVQVGSSVKPGQTLCIIEAMKIMNEIESESSGKIAKILVENG
                     QPVEYNQTLFLIEKA"
     gene            17869..19395
                     /locus_tag="PK754_03015"
     CDS             17869..19395
                     /locus_tag="PK754_03015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB08505.1"
                     /translation="MSFRKKIYITGFLLITTWLFAGFYLLMHYQRTQELTDTLIPYEI
                     VKLKSIENLRRHADSFERFWAPTEETPIHPIVLNQELIQFDEEILSLIKLHWESKALW
                     EAHGDHVGSSSLVMYVTGLIWYDTLSVDSERILLSHLKELAAQLRNSAPTRAGTTIAL
                     TEKKILGTIHEIQVSIHALDETANKILEQKISHISHRGLFAKSIIFTVFTLAIILITF
                     LIIDIHRILQSIGGIKTITSAFAEGKLEERLPVEGFEEIRTIAISFNAMGERMQELDK
                     LKSDFFNKLVHDFKSPLDNIKQSADVLISGITGETSDGKQKKFLEIIKKSSIQLRTMI
                     QEQLDESKLIAGQSSLVYEQTDLRQLIRERMELQRPTATARQLNFAVKFTDATYVIPC
                     DRAKLSRVMDNLLSNAIKYAFPETTISVEIEDRAHEIELRVRDHGPGIPATMLTQIFQ
                     KYVRIEHNRSTSGTGLGLYTAKYIVELHKGRIWASNYNDGAVLHVLLPKSPGQTRLNH
                     "
     gene            19460..19533
                     /locus_tag="PK754_03020"
     tRNA            19460..19533
                     /locus_tag="PK754_03020"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19494..19496,aa:Arg,seq:tct)
     gene            complement(19698..20429)
                     /locus_tag="PK754_03025"
     CDS             complement(19698..20429)
                     /locus_tag="PK754_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNB08506.1"
                     /translation="MIQSQLKLVYRKYISYAVGISIAVHLMAVGLYMAITLWDWSDGK
                     QEAPMVFKLENLDFLLNDSKPKSSGSRASMRAKVSSDKDLKKMANVSEIVKDEKEADK
                     KITDSMVVDTTAQFFAENGTGNGLGRGIGMGVGDEGGDGASSGKEKPVVHTNPRVRVL
                     VQPNVEKVKNKINGSVRLRLHIDNQGNVVEAVVVNNTTKDKELQEIARKTANRMKFYP
                     ATNDGVPAEDWFDHEIVFNFDGKKL"
     gene            complement(20513..>20955)
                     /locus_tag="PK754_03030"
     CDS             complement(20513..>20955)
                     /locus_tag="PK754_03030"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNB08507.1"
                     /translation="YWKKGDILTIDLLPNKTIEQLLDQYMDSRSDLVNVLSYHFPKRF
                     IQSWCVEHFSNKPVYQLSSKELNNLDEKIHRWKLIPVATAGFEKAEVTRGGVDTRDIS
                     SKTMESVKQNGLYFVGEVVDVTGHLGGHNFQWAWASGHAAGLFV"
BASE COUNT         6194 a         4794 c         4270 g         5697 t
ORIGIN      
        1 agcttgaccg tgtcggcgcg aatttcttga aagtcactga gcaaatcaaa aaagacacag
       61 gagtaaccaa caaatccgta aaatcgcccg agatacgtta tgccggcgta ctgggcgcag
      121 gcgtcatggg tggcggaatc gcctatcttt tttctaacta tgatatcgaa gtgcgactaa
      181 aagacatcaa ccatcaagcg atcacattgg gtctcgactc ggctaaaaag gtatatgagc
      241 gggcgttgaa acggcgtaaa ctaaccaaac aaacgttcag caggaaaatg tcctatatca
      301 ccggcgcatt ggattattcc ggttttcgcc gttgcgatct ggttgtcgaa gccattgtcg
      361 aaaatatgga tgtaaagaaa aaagttatcg gagaactttc aagtcacgtc agcgatagct
      421 gtatcgtagc gtccaatacg tcttccttat ccataaccga aatggcgaag gcttataaac
      481 atcccgacaa atttattgga atgcattttt ttaacccggt acataaaatg ccgctcgttg
      541 aaattattcg cggagaaaaa acatcggatg caactgttgc cgcgatctat gcgttgtcaa
      601 aaaaactcgg caaacaacct atcgtcgtga aggacggacc gggattcctc gtcaatcgtc
      661 tcttgatgcc ctatctcaat gaagccgggc atctgatgga gcaaggcgtt accatcgaac
      721 tattggataa aaccatgctc gattttggta tgccgatggg cccggcgcta cttatcgatg
      781 aagtcggaat tgacgtagcg tacaaagtcg gccatatttt ttatgatgca ttcggcgaac
      841 gcgtacaacc ttcccgcgtt gtcgaaaaac ttcatgaagc caaacgcctc ggaaaaaaag
      901 gaaaattagg attttataaa cacgcaggcg acaaacctca ggttgatcct gaagtctatg
      961 cgcttataga agtaaaccaa acttcgacta tccgttcgga agaaatggtc gaacgcatga
     1021 cttatacgat gatcaacgaa gcggccatgt gtttggccga aggaattgtc agccaaccaa
     1081 gcgatgtcga aatcggaatg atcttcggaa caggttttgc cccatttcgc ggtggccttt
     1141 tgcgatacgc cgacactgaa ggccttgata aaatagttgc acagcttgaa aaatatgcgc
     1201 acacttacgg cgtacgtttt accccatgcg attatttggt acagttacaa aaatcaggta
     1261 agaaatttta taacgaataa ctaaaacaaa caaacgaaag gatcgaatca tgcttggcga
     1321 taaactcatc agcaaaggtg ttatcaatca atcccaattg gacgaatgcc tcaaagaaca
     1381 acaaagcagc ggtaaaaaac tcggtgaagt tatcatcgcc aaaggatatg cgacgcaagc
     1441 tcagattgat gccgctttag ccggatagac ttcggattat cgcttgaaat cttttgcaca
     1501 gccgcgtata ttcgaatgca tttaacattt cattttatat tcaggcccag cccttatgga
     1561 taaagacctc atcgctattc aggaagcacg cgacatcgcc cgagccgcca aagctgcaca
     1621 aaaagagttt gcaaaattta cgcaacaaaa agtagatacc cttgtcaaag cgatggccga
     1681 tgccgggcta gccgccgcag atcgtttggc gcgtatggcc gccgacgaaa cgcgtatggg
     1741 acgttatgag gataagattg taaaaaatca atttggcaca cgcgatgtgt acgaatacat
     1801 caaggacatg aaaacggttg gtgtcttgcg ctacgatgac aaactcaaat tttatgagat
     1861 cggcaccccg atgggtgttg tatgcgctat aatccctacg accaatccca catccaccgt
     1921 gatgtacaaa atattaatat cgcttaaagc cggtaatgcc atcgctatcg ctcctcatcc
     1981 gcgtgcggcc aagtgtacct acgaagccat gcgcatcctc cgcgacgttg cggaatctat
     2041 gggtgcgccc aaaggcctca tcggttgcat cgaacatccg acacttgaag gaacacaagc
     2101 attgatgaag tgcccggaga cttcggtgat tttggctacc ggcgggcccg gtattgtacg
     2161 cgaagcatac agttcaggta aacctgctct gggcgtgggc tccggcaatg ttcccgcttt
     2221 tatcgaaaaa acggctaatg ttaaaaaagc agtcgccgat atcgtcgcgg gtaaatgttt
     2281 cgattacggt ttgttatgtt catctgaaaa ctccatcgtt gccgacgctg cactgcgtga
     2341 tcaggttatt actgaattga aagccaatcg cgcatatgtt tgcaataatg aagaaaaaat
     2401 taaacttcag caactgatgt ttcctaaagg aaaattgaat acccagatta tcggccttcc
     2461 tgcacctaaa attgcgcaaa tggccggtgt agcggcacca tcggatgcga cagtactggt
     2521 cgtcccttgc gaaaaagtcg gcaaagaaga acccctgtcg gccgaaaaac tttcaccggt
     2581 attatcactt ttttgggtaa acgggtggga acaaggctgt gaccgcgcac tggaaattct
     2641 caatttcgga ggtataggac atacgatgtc cattcactct caggataatg atgtgatcat
     2701 gaagttcggt ctcgaaaaac cggccttccg tatttgcgtg aataccgttt ccaccttagg
     2761 cgctgtcgga tatacgaccg gactcgcgcc gtctatgacg ctcggacccg gcacactcgg
     2821 aggctccatt acgacagata atatcatgcc gtatcatttg atgaacgtga aacgccttgc
     2881 ctttgaaata cgcccgttca aatcttcttt agttcacggc aataccgaat cacaggcaaa
     2941 agatacagtc aaaaaaaccg ccgaaatacc gtctgccccc gtttcttctt cgtctaagaa
     3001 cgattctttg tcggaaaaag aaattgataa aatcgtagag gaattcattc gcagcagaaa
     3061 atagcctata aaattctatt gcaaattaac gtgttttacg tgacattgca ccagctttta
     3121 agcacttcgc acaacgtgaa gtgctttttc attaaaggag atagcacacc tcgtggttat
     3181 tgatatctta gtaatcctta tactcgtttt actcaacggt ttttttgtcg ccgccgagtt
     3241 tgctatcgtc aaagtgcgtt caacgcaaat cgacaccatc gaacaaaaaa acatccgcgc
     3301 acgcatcgct aaagaagtta ttatgcacct tgatgcgtat ctgtccgcaa cccaacttgg
     3361 tattacgatt acaagtattg cgctcggctg gatcggcgaa cccgctattt cacgcatgat
     3421 tgaacctatt atcaattatt ttggaataac caatccgcaa gtgattcatg ccttgtcttt
     3481 tggtatcggt ttttccgtga ttactttttt acatattacg atgggcgaac tgggccccaa
     3541 atccattgct atccaatatc ctaaacaaac aacgctttgg attgcatcgc cgctccatct
     3601 tttttatgtc gttttcaaac cattcatatt catactcaat aaaagcgcta atctcatgct
     3661 tcgtatggtc gggattcatc cagccggtga acatgagtta tctcactccg aagaagaaat
     3721 tcgtatgctc attgctgatg gtcgtaaaag cggtgtgatt gatgctacgg agtacaagtt
     3781 aatcgaaaac attttcaatt ttaccgaaac ttcggtaaaa gaaataatga ttccccgcat
     3841 ggatgtattt gccttggata tagagaaacc ggttgttgaa aatctgaaat cggcgataga
     3901 cagcggctat acgcgcatac cggtgtaccg tgagtcaatt gatacattga tcggtattct
     3961 ttatgtcaaa gacttattca aaattgaccg cgatacgata acgaccgctt ccttcgaaac
     4021 gatactacgc cctgcttatt ttgcaccgga aagcatcagt atcaatcgtc tgatgcagga
     4081 ctttcttcaa cagcgatttc atatggcgat tatcattgat gaattcggtg gaacgagcgg
     4141 tgtcgtaacg cttgaaaaca taatcgaaaa aatagtcggt cagcagattc aggacgaata
     4201 cgacgatgaa aaaaaggaat ttgaaatttt atctgacggc agttatacgg tacaggccaa
     4261 gatgcgaatc ggcgatttta atcagcaatt tcaggccaac atacccgagg acgataacta
     4321 cgaaacgtta gccggttttc tgaataatgt ggcgggccat attcccaata acggagaaga
     4381 aattcggtat caaaatctaa gtttcaggat tacaaaaaaa tcaccgaagc tcgttcaaca
     4441 agtgcgtttt gccaaactta cttaatcggc tatgcttcaa actttcgaaa gccggtgttt
     4501 agataaccgg cttttttctt ttttgagtgc cattaatatc gcatttatag aggcatccac
     4561 agacttttca ccggccgcta tgagttcgtt cattttctca aatgccgccc aatgatgatg
     4621 cccgacttca ggtcgaatat gtaccgttga cctggaacac ataagagcgt tggcttgcag
     4681 tgctgtgata agatgagttc taaataataa atcaaccata ttcaccggcg gtgcctctcc
     4741 ggccagagta cgcgacgtat caacagcgat cacatagtca gcccccatct cccatgccgc
     4801 atctaccggc gtctgcgcag cgacagcgcc atccactaag actttatctt cgtattgcat
     4861 cggcggtaaa aaacccggga tagatgaact ggccttcgcg gcttctttga tcgaaccttc
     4921 tctgaaaatt tcggtttttc cggtgatcat gtttgttgca acagcggcaa attgaatgcg
     4981 ggtattttcg attaaaccat ctcccaacaa ttcatccatc acgatgccga gtcggttttc
     5041 tttcatcaaa gacatccgat cggcgtatag ctgaatcagc aactcattcc ctaccgcccg
     5101 ggctatttgt ccgaaaaaat tttcaggatg catatcgcgg cgaaaaagat gcagtcccgt
     5161 ttttttaaaa tcatcgccga gaaagaattt ttttatccga gactccacct cagcaatatg
     5221 caaagtctga gcatacatcg caccgacaac agcgcctata ccgctaccga cgatgatatc
     5281 catcggaata tgatggcgtt ctaaagtttt gagtacaccg atgtgggcca aaccgcgtgc
     5341 gccgccgccg cccaaagcta atgcaatttt ccgttgtcca aacattcgtt attttaaaac
     5401 gatttcgcgt ttttctttcg ccgattgata aatcgcttcc gaaatgcgca tgatctccat
     5461 ggcttcttcg ccgctggaca tcgaaggccg gttattttta atgcattcca caaagtgttt
     5521 cagttcgtat tcataagatt ttttataatt attttccggc ttttccattt tttgaggcgt
     5581 cacattgaca agattgccgt gcatttcttt gtgaatacgt agcggattga gcaaagcgcc
     5641 cccgcttgtt ccaaaaagat tggaatacat aaaatcattt tccataagta atgtccagct
     5701 gacttcgatg gaaagcacga catcgttttc aaaacgaatc attaccgccg ccgaatcttc
     5761 gacgccgtct ttctgaaaat ggctgtacat cgtgctaaaa acgctatgaa ctttaggatt
     5821 gtccatcagc catatggaaa gatcaagcat cggaattccc agatccataa aaacaccgcc
     5881 gccggaaaca tttttctttt tggcccaatc ggtttcattc cagctgccgc gacgacgcag
     5941 ccaacccgat ttggtgtaaa atattttacc gagttcttta cctttaacga aatttttaag
     6001 taccatcgca tccgggcgga aacgatgatt cattcccacc ataagaatct tcttccgttt
     6061 tttggattct tcgaccatgg tcttggattc ctcataggta agcgcgatgg gcttttccac
     6121 cagtacatgt ttccctgcgg ataatgctga tttagccatc ggcgcatgca tactgttagg
     6181 cgtacatata tgaacgccct gaatttcatc cattgctaaa agtttttcaa aatcagtaaa
     6241 aacattgggg atatgatact tctccgccag cgctcctact ttggttttat ccgaatccgc
     6301 gatagccacg atttcaacat cgttcatttt cgaaagtata ggtaaatgga ttacctgtga
     6361 aatagcgccg gcaccgacga ttcccaattt cactttttcc gtattcgctt tgcctttgtc
     6421 cttgggcata aaacctccgt aagaataaaa ctattgatga accgccatgc ggtccgaaga
     6481 taaggatata tttaatgtac tataaaattc agggaaagaa atacgcacag cctctgcaga
     6541 atgtacgatg gattcgccgc cttcagcaat caaagcggca actgtaaatg ccatcgcaat
     6601 gcgatgatca tcaaatgaat ctatttctga cgatcgaaat gaaccgggat aaatacgaaa
     6661 tccgtcatca aattcatcaa ttcgtattcc catagaccgc atgtttttta caatggcttc
     6721 gatacgatca cattctttat tgcgtagctc acgtgcatca cggacttcca aaccatcttt
     6781 caaatgggtt cctagcacgg ccaatatcgg aatttcatca atcaaagcag ctacaaccga
     6841 gccgcttaat tgtactgatt ttaattcgcg ggtgtggcgc gtgacgatat caccataagg
     6901 ttctcgcgcc tctgtgatat tatgaatagt aatatccgcg cccatttttt tcaaaatttc
     6961 caaaaaggca atgcgagtag ggtttagtga aacatggcgt atagtcactt cgctttcagg
     7021 cagcaagagc cccgtcgcaa ttaaaaatgc tgccgaagat aaatcgcctg gaatcgtcat
     7081 atgatcaaaa cgaaaagatt cacctcccgt aaccgacaaa ctaatccggc catcttcaac
     7141 cgattttttt atcgttggta gaaggcgctc tgtgtgatcc cgtgtcatga ccgattcgcg
     7201 aacggtagtt attcctttag cataatgacc ggccagcaaa atacaacttt tgacttgagc
     7261 gcttgcaatc ggtaaactgt aatcaatggc ttttaattct gtaccttcga tttctatcgg
     7321 taatgtttga tgcgccgatg tttttatttt ggcacccatt tgagtcagcg gttcaataat
     7381 gcgtctcatc gggcgtcgtg ataaagacgc gtcaccacta agttcagatt taaaactttg
     7441 accggaaaga atacccgcca atagtcttgc cgttgttcct gagttcccgc aatacaaagg
     7501 cgcggatggt gccacaaatc catgcatacc gcatccggtt atgtgcaact ctgaaccgtt
     7561 ttgatcaacc ttcacgccca atcggcgcat acaatctata gtactttgca catcagcgcc
     7621 ggttgctata ttaaacaagg aaacgatgct ttcagacatg gctgaaaaaa taactgcacg
     7681 gtgtgtgata gacttatcac cgggtattct acaatcaatt ctgatctttg tcgcaggttt
     7741 aatgagcgcc gttgccataa catttcatgc aaataattga taacaaaaat acacatgcaa
     7801 cgatgaaaaa gcaacggtaa aggggcttta ttcaagccaa atcgggttag tgtagcagaa
     7861 attttgactt ttcgtagtaa cgctcaaacg tatataactc ggcgcgtctg ccgtcgtgtg
     7921 aaactctcct tgatcgcacc atgattcaac cgtcatcgta gagacctttt cgtgcgattc
     7981 gccgatgact ccttgaaaga cgctaatcgt ttcgagcgca ccaaactctt gtgtgctcga
     8041 cgcttcccat gcaacgcgaa accgtcggcc tttagcgttt tgtccgatgc ggctagaaaa
     8101 acctgcctcg tttaaaacat taaaaacaac gtatggaccc gtcgaaataa tcgcacatcc
     8161 ttcacgaatg gcatcgatcg tattttgcgt attaggtttt ttttcaccga ggtacaatac
     8221 ggttcgcgcc caacccagtt gtttgtggtg cgatatatct tcgatggtcc aaaaaggaat
     8281 acgcacctga cggttacgat taaagttgcc atgcgcatcg tttccggcaa caatataaac
     8341 ccgtcggcct gacaacaaaa gatgcaccca caagcccaaa ccttgcttga atgccgtatc
     8401 gttactacca tttaaaattt gtaagacctc cgccgaacga ttataaaaat cttctaaacg
     8461 ccatatcccc cgttgtaaaa aagcacgctc cattggagat ggtttttcta aaggatgcgc
     8521 agccataata agagcttctt tattcaataa acgggccatt cccgataccg tatgttcgga
     8581 ggatgtgttt agtggatttt tacctccgtc gccggaacca taaaaaaaat gatccgagtt
     8641 aagaatcaac gcatgtatat tttcatcggc aatattcata catgatagct cttcaccggc
     8701 cagtaataaa acttttgaag aggattcttc attcatctgt ttcacggatt cgataaatgt
     8761 acgccattgc gcaagcgtag ggtcggattg attataatcc tcttttttat tatccaagtc
     8821 ataagaatgg tctgtcacgg cgataaaatt taatcccaat gcctcgctcg cagctctgag
     8881 catgggcagc ggagctccat attctatagc atcttcggta tagtcgctgt gtacatgcaa
     8941 atcaccgtta aaccaccctt ggggttgcgg ccaatgatcc ccgctcacat agacatcaaa
     9001 cggcgccttt gttgttccaa ttatattgtc ggcttccacg gtatgcgtga ccccggctgt
     9061 tctgtattcg acaacaatac gcagttgaac ggtatggcct atccatgatt cgtttacggc
     9121 gatatcccac tttttccacc accaatgttg gcgtatttcc tcttgtacct ggaaaatcaa
     9181 ttcatcacta tttgcgcctt ttgtaatgat ggtacgaact gagagaatgg acgtgggata
     9241 gagatgcgca tctttgacta aaacaaagac cggcatcctt cgaccacgct ctatacgatg
     9301 tggaatatcc gcaatgattt caggttcttt tttgaaatat cggctgaacg gaaagaatcg
     9361 gaagcggtaa tgaatttctg gatataataa aaaaaacgga agaatcagag aggacgtggt
     9421 ttgcagggat tcaagagtta aagaagtgaa cgtcatgaat tagtgtcgaa gccgtcacga
     9481 aacgtattta atcattttaa cacgattttt ttcaccggga tgaatatcga aaacaacttc
     9541 atccatcatt cgtttgatca gcgctatccc aaggcctccg cgcgcatgtt tctgaagacg
     9601 ctgttgaggc gtttccagac gatccggatc aaacccttcg ccttcatcgg agataatgat
     9661 ggtaaattta ctaccgttgg tttctaccgt aatatcaata tttttggatt cgtcatattt
     9721 atacgcgtgc ttgatcacat tggcacatgc ttcatcaacg gccagttcga tttcgccgat
     9781 cgcatcatca ttaaatccaa accgtcgggc gatcgacgtg ataaactcac gaatgatccc
     9841 tagattgtcc gtacggctcg ggatggccat attaaatgaa tttgttgtaa cgctggacac
     9901 cgttgtttcc acgattatta tttattggcc tgtacaaagt gatcgcgcgc ttccgaagga
     9961 tctttgagta tctgataaat cgtcggaaaa ccgagcaaat caaaaacttt atatactttg
    10021 gtggacatcg tacacagttt gatatcacct tgattttcgc gaaccgtttc aataaagccc
    10081 ataaaaacgc ctaacccggc gcttgaaata taactcagct cctgaaaatc cacgacgatc
    10141 cggtaacgtt tttcttccat caatttttga agctcgcctt caaaatgcgg atacgtgtgc
    10201 gcgtcaagat atcccttcaa gtacagaaca gtgatatcct gctcgtcttt tcgcgtgact
    10261 tcgaacccgt tcatggttta ttcctatcgt tataaaatga aaactgaagt gtcgcttttt
    10321 gaaaaatatt gtacttcaat ttttcatcta tttcaagcac aaatcaaacg cgtcgtacgg
    10381 ccgtaatttc cccggtttcc gcttctgtga caggggccac cggaacaact tgtttctgta
    10441 aatcggccaa ccggaaaaca acgcaagtca aatcatcgtg tgttttcgcc gagccgacaa
    10501 accgttttac atccgctatg attttatctt taataacccc ggccggaaga tgctcgacac
    10561 cgtcaagaca tttactgagg cgctcttcac cgtactcttc atccttcgca ttacgcgctt
    10621 cgaccaatcc gtcgctgtac atgacaaaaa catcccgatc gtcaattcgt attgtctttt
    10681 cttcgatagc ctgacgaaag agattacctt tatccaatcc taagccgatg ccattgggct
    10741 gaacataggt tttgacacca tcacgcatgt gcagtaaagg acagtggccg gcccgcgcaa
    10801 aacgcagttc gctcttagat atatccacat ccgcatacag caacgtaata aaagatttgc
    10861 gatcaatcgt accgaaaagc acgtcattaa cggcgatcaa aaggtcttta ggggcggaat
    10921 aaataccggc taaggcttga attatgcctt tgatctctgc catataaaaa gcggccgagg
    10981 taccctttcc ggaaacgtcg gctaccgcaa ttccgcagcg ccagtcggcc aaacgaacaa
    11041 aatcataata atcaccccct acttcattgg caggtactgt cgtcgcgccg atatcaagcg
    11101 tcattttaga tacgtcatgg cgtatatcgg gaatttcctg cggtataagt ttcatctgca
    11161 catcatgggc gatgcgcaat tcttccgcaa gacgttcttt ttcaagtgac tctttgacca
    11221 aacgcgtgtt ttcgatagct accgtcgtct gattggctaa cgctgatatg atcgcaatgt
    11281 catcctgatc aaatccgaac gaagttgatt tgacggcaaa aataatcccg agcatgccgt
    11341 ccgacgtgat cagaggcaca ccgacaagtg actcgatggg tgtgcgcttc agatcttttg
    11401 ttcgtttatc gcgtttgact tgattgatca gaatcggctg tttgttattg atgatccaat
    11461 ccgtcggttc ggtcagatag taatggtgat gctgatcgtt ggatggtatc aatcttgcat
    11521 tttcatgttt agaaaggaat ttacgaaaac taacttcgag aaagtgaacc tgaaacggtt
    11581 ctctttgacg tacagccgca aaatcaaaat gggtacggaa ggcagggttt tttgtgttgg
    11641 ccagttctag ccagcacgca ttggcgcctg tcgcatcgca caccaattcg gtcacggaga
    11701 tcacgatttt ttccacatca aagagcgaat tgatcgtacg gctcagcgaa taaattgaat
    11761 tgatttcgcg aactttttta tcataaatag cggccgtcgg caagtgcagc agtgtattga
    11821 tcgtcgtact gatactatat acaaaaacaa aaaccgtcat taaaaaaaga aaactcccgg
    11881 ctgtacggct ataggaatac accatgtcag cataggtggc tccgccacca atctccgcac
    11941 ccaaaagtcc ggccgaaatt agggtaaata ccaaccctcc tataaatgta agaaacttct
    12001 gacgacggtt gagaacagtt acccacgtca cacgtcccgc attgatcaga agaaaaatag
    12061 tggtaataac aagaaggtta aatgtgatga tgtgattaga ggacggatcg tacttcagtg
    12121 ggcgtccggt aatcgcggtt gacaaggccg tcaaagcgcc aaaaagtaag aacagatgaa
    12181 aaaaagtagc cgtatttttt tttcgacgat agaaaatcaa tgtctgcaaa atccgaaggc
    12241 atgaacccag cgacaatacg gccacgaatc cgatcacatg ggcgactatg acacctgacc
    12301 atgtataagc tctgagtaca taggagccag agtaacgcgg cgtgaaaaat acgggctggg
    12361 tgacgagcgt catcaacagg cccacaccat agatcgttac gacgaaaaaa agcgataatc
    12421 gcaatttttg tgtgatactg attgattcaa accgagggta attaaacgct acgtacaaaa
    12481 ccaaaccaat aatagcaata ctcagaagtt cactgcccca gtcccccgca ctaaaacgaa
    12541 ttttcgcata gaacataaac gaattgagaa tcaccccgat cacgaagaga atcggaataa
    12601 aaatcaatgc gacacgaaac aatttgttct taggatccat gcaacccgtt ttatttcaaa
    12661 aatgctatct aagctggcat acgcaaaaat aacacatagg tttccaataa tatgaactta
    12721 cgataggcca attacgattt caatacgaaa ctcacacgcc tattcgaaga atgatttttc
    12781 cgatattaag attagacttc attcgcatat gagcggcctc tgcgtcaaac catgaatatt
    12841 ccgaatcaat aatcggacga aataaaccgg aagtgaaatg cggcatgatc agttctctca
    12901 tactttttgt cagatctgat ttttcttcat gcgatttgtt acgcaacgtg gaaccgataa
    12961 tggaaatgtt tttggtcaat aatgtacccg tcggtaaatt ggaagacgta ccacctaata
    13021 gcccgattac gacgatgcgc gccttgttgt tggcgacctc tacgtgatgt gacatatttt
    13081 ccccgccaac ggtatccaat acgacattca cgccgccaca acgcgtcttt ataagattcc
    13141 aaaaatcttt ggcgtgataa tcaaatacaa aatcggcgcc taattcataa caaaagcgca
    13201 ttttctcgga gttgccgaca gaagctataa cctttgcatt taaattttta gctaattgta
    13261 tagcagcagt tcctacaccg cttgctccgg aatgaatgag gatggcttct ccggctttaa
    13321 gttgggcgat cgtgacaaga ttgagatacg cggtaaacca ggcttccggt atcgctgcag
    13381 cctgagcaaa actcaacttt tccggtatcg gtatgatcat gttttcatga acaactgctt
    13441 tttctgcata tccgcagccc ggcaaaagag cacacacgcg ttgccccggg ctccactgcg
    13501 tcacatttga gcccactgaa agaataaggc cagcacattc caatcccaaa atatcgggaa
    13561 cacctgccgg cggcggataa agcccacgcc tttgaagtag atcagcacga ttgagagctg
    13621 ttgctttgat tgcgatcaac acatcatttg gatgaaccat gggttcatca acttcactga
    13681 taaccaatcg tgatgtgttt ttttcacgtt ctgcaagtat ggctttcata aacaattaac
    13741 aacagaaaag taaagatatt ttccaaatca aaaaactaat aaatatccaa tgaaattttg
    13801 catgttgtga tatacgtcac agcacgagcg aagcatttat aatagcttga aagccgaagt
    13861 gtcactatac caaatctaag acatttttaa ttaatttcaa ctaaaacaaa aacttaaaat
    13921 gaacgtcggc acgcaacatc atgtctccga taagaaacta agtgcttcaa gtaagagcgg
    13981 tgtggtgtgg cctgcactga ctgaacgtga aattatacgt attgctgttg aagatacaac
    14041 ccgttacgaa cccgccatgt cagaaatatg ggaacgctac aaaaaacgtt tgtatcttta
    14101 tataaaatat aaactcaata ttaataaagg ttttgaaact gataaagaac gttacattat
    14161 cgaagatatt cttcagaata ccttttgtga tgttctagaa aaccttaatg aatataatcc
    14221 tcaatatgaa gtttcaacat ggatttataa tatttgtaac aagcatattg tacgttatat
    14281 acgcgaagtt cagaaatatc aaggacgtac tatagctatc gaagacgaat ctgtcgaggt
    14341 tatccgatta tcctccgact ctgtgaagcc tgaccaagaa ttcgaagtaa aagaatttga
    14401 acgaatcata accttttttg taaaatcatt acagcacaaa gcggatcagg aagtgttcct
    14461 tttgtattta cagaatttac ataatcataa catttccgat cttttgggta aaacgcaaga
    14521 cgcggtacga gcccggctca atcgcgtgat caaacggttc aaaaaattta tgactaaaca
    14581 gtatcctgaa tatctaaatt caacgatgct gtcgcaaatc aaaaaccttt cgtttggttc
    14641 ggccaatact gaaaatgatt tttcacctga aagaggatag gtggtaagcg gattatgaag
    14701 caacttcaaa acaaaatcga agttacggct gacgaactgt ttgaacagtt tgttcgcgaa
    14761 ctggaaaatt acagcaccaa tggcgttgtt cgttcttttg aaacggaccg tctttggccg
    14821 cttgctcagc actacattgc gcaatgcgca cccatgcaag agcgtatgga tttagaggaa
    14881 cgaattcgcc ttctcttagc atcgaaatat cagtacactt tgcaggaatc ccgattaaaa
    14941 ctcagtgaaa atttagagca aagtatcaaa gaaaaaatca gggccttagt cagatctaac
    15001 atagaaaatg ccggtaaatt tgctgcgtta gctttcaatt ggacagtcat aaagcctttg
    15061 cagtttgcaa aggatatttt acagccggtc gcaaccaatg ctatgcttac ctatgctgta
    15121 gcgattcaac catcattcac accggctatg gatgcgatac aactagagtc taaaaagcaa
    15181 atctcgcata tagaagtcgc tgacgaaatt aagactgatt ttttacgagt ttataaacta
    15241 ccaagcgtaa aaacatactc taatgaagat ctgaatgcca tgaacgccgg agccaataca
    15301 ctggcagacg aaaaagctcc gataattcag aaaaatgtaa ataagacgta tcacataaca
    15361 cctgtacaat cgtctattaa agagacttcc aagttgacca ttgatgtgga gcgtgctcgg
    15421 ttgattccgg aaagcatacc ttcggtggct tcttcccccg tttcttacga ccgtacacga
    15481 cgtgaattgc gatctatcgg tgaagttcaa caaactatcg gcctgcatga aaataaaatt
    15541 tttagttgtt atcagtcgta tcgcaaaact tcaaatagaa aaaatggacg tgtcgccgtt
    15601 aagtttttag tttcgagtaa aggggttatc aaggatgtac aggtaactgc caatagcttt
    15661 aacctgcaaa tggcggaccg tattgtggta caactcaaag ctatcaagtt tagcgaagtg
    15721 gaatcacgcc ttggcgacca gactatatac catacgttct atttctgatc gaattatttg
    15781 agaaaataag tagagagggg ttacttttat gctggagtac atgctactca agtacgggct
    15841 tatttatatg ccggcttcga tcaatattat cgcaaagcgt atcgggatat ccgaaaccga
    15901 tcgcaatctc gaagttctca aaaaagaatt ggataaactg gtttcaaaga aaaaggctta
    15961 tcgctccggc aaccgctatg cgatagatgc cgaaggtgca cgcgccattt cagagtacca
    16021 ggatccaaaa aaactgcagg aaaaacctaa tccggaaaac tttaaaaaaa tttttaagca
    16081 gtattctgtc gcacgctagg aaaaacgtgc gaattttaag aaagcctgac gataaccgtt
    16141 aggctttttt cgttttagag gattttgctt tgtttgtacg caccgcagat ttcttttcgg
    16201 tatttgctcg ttttgccata tcaattacta cagaaaccaa gcagttttcc gtaccaaaag
    16261 gattggtttt acgcggcgct ttatcatcta ccatccaggc ttttttatta acacgataaa
    16321 gaaattcgta ctcgcctgtc gtcaatttta atgttataga ccagttaccc gatttatctt
    16381 ttttgagagg atgtttatct tcattccaac cgttaaaatt tcctaaaaga ttgaccgatt
    16441 ccgcctcgct taaatgaaca gaaaaagtcg tcttccccgt tttcgagtct actattacca
    16501 taagttgctt cctctcattt tagataaata acttaatgaa tatacctaca aatcataaaa
    16561 acacaaggaa aagcagaaaa aattacaaaa ttaatgcaat ttcgcagcat ttacccggca
    16621 aaaatatacg tttttataaa attcttgact ttggatatgc cattctctac tttgagcggt
    16681 ctaaaaaata caatcgttta ctttctttag gattcagatt tacatggcag gtgtcgccga
    16741 ttttcgaaca ggtatgacca ttttgatgga tggtaatctt tttgttgtta ctgagtttca
    16801 acatcgcaat ccgggcaatt ggcgcgcttt tgtcgtcgct aaattaaaaa atgttcgcac
    16861 cgggcgagtg atcgaaaaga catttcgttc gcacgacaaa ttagaagaag tacgcgccga
    16921 aagcaaaaac atggcctacc tctattccga tggcgatatg atgtacttta tggataacga
    16981 atcatttgaa caagtgggta taccccgtga atccgtcggt gaggcggcca actacctgaa
    17041 agaaggtatt gcaccggcta tcctttttta tgaaggcaat gccgttagta tcgaaatgcc
    17101 tatcaccgtt gaattaaaaa tcacaagtac ggttcccgga gttaagggcg ataccgtgac
    17161 cggaggcacc aaaccggcaa ccttggagtc cggtgctgta gtcaatgtac ctctttttat
    17221 caacgaaggt gatacggtga aagttgacac ccgctccggt gattacctcg aacgcgtcaa
    17281 tacgaaataa atcttctgca caaacggaga gagaaacgca tggatctcaa agacttgaaa
    17341 aacctcatca aaatggtgga tgagagtaat gtcaatgaag tcgaaataga acaagacaac
    17401 gccaaaacca aaggacgcct gaagatacgg ataagcaaat ccggtacgac acatgccatg
    17461 gccgtcgttc aacattcggc tccgatggct atgccacacc atgtaccgac acaacatctt
    17521 cccccgccgg ctgtccaatt tgaaagccaa gagacgatag aaaaatctaa atccgaaaga
    17581 ccggccggag ccgtagaaca aggtaatttt atagaggtca agtcgcctat tgtcggtact
    17641 ttttaccgtt cgcctgcacc cgacgccgat gtttatgtgc aagtgggctc ttcggtcaaa
    17701 cccggacaga cgctttgtat tatcgaagcg atgaaaatca tgaacgaaat cgaatccgaa
    17761 tcatccggta aaatcgccaa gatcctcgtt gaaaacggtc aaccggtgga gtacaatcag
    17821 acgcttttct tgatcgaaaa agcttaactc ccggcgagaa taaacgccgt gtcatttcgc
    17881 aaaaaaatat acataaccgg ttttttactg ataacaacct ggctttttgc cgggttttat
    17941 cttttaatgc actaccagcg tacacaggaa cttaccgaca cgctgatccc ctatgaaatt
    18001 gtcaaactta aatccatcga aaatcttcgc cgacacgctg attcattcga acgattttgg
    18061 gcccctaccg aagaaacgcc gatccatccg atcgtcctta atcaggaatt aatccaattt
    18121 gatgaagaga tactgtcgtt aataaagcta cattgggaaa gcaaggcgct ttgggaggcg
    18181 catggggatc acgtcggctc atcatctctt gtcatgtacg ttacgggcct tatttggtat
    18241 gacacgcttt ccgtggactc ggaacggata ttgctgagcc atctcaaaga actcgcagct
    18301 cagcttcgta actccgcgcc aacccgtgca ggcactacga ttgctttgac agaaaagaaa
    18361 atattaggca ctatacatga aatccaagtt agcatccatg cgctggatga aactgccaat
    18421 aaaattctgg aacagaaaat ctcacatatc tcgcatcgcg gtctttttgc caaatctatc
    18481 atatttacag tttttacact tgctatcatt ttgattacat ttctcatcat cgacatacat
    18541 cgtattttac aaagtatagg cggtataaaa acaatcacat ccgcatttgc cgaaggcaaa
    18601 ctcgaagaac gcctgcccgt agaaggtttt gaggaaatac gaaccatcgc catatcattt
    18661 aacgccatgg gcgaacgcat gcaggaattg gacaagctca aatccgattt ttttaacaaa
    18721 ctcgtacatg atttcaaaag cccgctggat aatatcaaac aatcggccga tgtgctgatc
    18781 agtggaataa ccggcgaaac ttcggacggt aaacaaaaga aattcttaga aattattaaa
    18841 aaaagttcca ttcaactccg tacgatgata caagaacagt tggatgaatc caaacttatc
    18901 gcggggcaaa gctcgcttgt gtatgaacaa accgaccttc gtcagttgat ccgcgagagg
    18961 atggaattgc aacgcccgac cgccacagcg cgccaactga attttgccgt aaaattcacc
    19021 gatgccacct acgtcatccc ttgtgaccgt gcaaagctat cacgtgtaat ggacaatctg
    19081 ctcagcaatg cgatcaaata cgcgttcccc gagacgacca tatcagtaga aatagaagat
    19141 cgtgcacatg aaattgagct tcgagtccgg gatcacggtc cgggcattcc agcaacgatg
    19201 ctaactcaaa ttttccagaa atacgtacgt atagagcata atcgaagtac atccggcaca
    19261 ggcctcggtt tatacaccgc taaatatatt gtagaattac acaaggggcg catctgggca
    19321 tcgaattaca acgacggtgc tgtattacac gttttactac caaaatcacc ggggcaaacg
    19381 cggctcaacc attaaaaaca aaatctgctt gaaacaggaa tcctgacact ctatattgcg
    19441 cgcgtcaaac atatatgatg ggcccgtagc tcagctggat agagcatctg ccttctaagc
    19501 agagggtcac aggttcgagt cctgtcgggc ctactaataa acaaaggttt gctaaatcta
    19561 tagcaaacct ttcttattta acatcaagat atcttttgta agataaccca tcaattcatc
    19621 ccgtaaatgc gcttgccgta ggttactgat tgaaaatttt atattaaaaa gtgaatatgt
    19681 gattcaagcg gccctgctta taattttttt ccgtcgaaat taaaaacgat ttcgtgatca
    19741 aaccaatctt cggccggaac tccgtcattg gttgcgggat aaaacttcat tcgattagcc
    19801 gttttgcggg caatttcctg aagttcctta tctttggttg tattatttac aacgacggct
    19861 tcaaccacat taccttgatt atctatgtgt aagcgcaatc gtacagagcc gttaatttta
    19921 ttttttacct tttcaacatt gggttgcacc aaaacccgaa ctcttggatt ggtatgaaca
    19981 acgggtttct ctttaccgct ggaagcgccg tcgccacctt catcccccac acccatgccg
    20041 attccccgtc ctagaccgtt accggttcca ttttcagcaa aaaattgtgc ggtcgtatcc
    20101 acaaccatgc tatccgttat ttttttatca gcctcttttt cgtctttaac gatttctgat
    20161 acattggcca tcttcttaag atctttatcc gaacttactt ttgcacgcat cgaagcgcgt
    20221 gatccagatg actttggctt actatcattg agaagaaaat ccaaattttc gagtttgaaa
    20281 accatcggcg cttcctgttt accatccgac caatcccaaa gtgtgatggc catgtataat
    20341 ccgacagcca tgagatgtac ggcaatggat atacctacgg cataggaaat atatttacga
    20401 taaacgagtt ttagttggct ttgaatcata tattaataaa acgaattctg gcacgaaatg
    20461 ttccctaaac taatatccgt gttttttttt attagcgcaa gattgaaaaa tattaaacaa
    20521 aaaggcctgc ggcatgcccc gacgcccatg cccactgaaa attatgccct ccaaggtgac
    20581 ccgttacatc tacaacctca ccgacgaaat acaaaccatt ctgtttaacc gattccattg
    20641 ttttcgacga aatatcgcgt gtatcaacac ctccgcgtgt aacttcagct ttttcaaatc
    20701 ctgctgttgc gaccggtatc aatttccacc gatgtatttt ctcgtccaaa ttatttagtt
    20761 ctttagaaga aagttgataa acaggtttat tcgaaaaatg ctccacgcac catgattgta
    20821 taaatcgctt aggaaaatga taactcaata cgttaaccag atctgaacgg ctatccatat
    20881 attgatctaa caattgttct atcgttttat tcggcaaaag atctatggtt agaatatctc
    20941 cttttttcca gtaag
//
LOCUS       DAOQNQ010000024        20623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_884_length_20623_cov_6.739012, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000024 DAOQNQ010000000
VERSION     DAOQNQ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 20623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20623
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_884_length_20623_cov_6.739012"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1628)
                     /gene="htpG"
                     /locus_tag="PK754_03035"
     CDS             complement(<1..1628)
                     /gene="htpG"
                     /locus_tag="PK754_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013506974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNB08508.1"
                     /translation="MSSQTLEFKTEVKELLHLMIHSLYSHKEIFLRELISNASDAIDK
                     VRYLSLTDSQISGGDADWKIKLKIDKEAGTLTISDNGIGMTEEEIIRSLGTIAHSGTR
                     DFVQRLTEKQVKDNPELIGQFGVGFYSSFMVADRVVVISRKAGEPQDKAIRWESKADG
                     VFTVESFEKATRGTDVILFMNEEGKKYLEEREIRSVVRKYSDYIEHPICMDVEKDKKI
                     EEETLNSRKAIWLKDKSEITTEEYNDFYKHVSHDFGEPLKTIHFRAEGTSEFSALLFI
                     PKKAPFDLFYKEFKIGPMLYVKRVQIMDHCEQAIPTYLRFVKGVVESSDLPLNVSREI
                     LQANKQMEVIKKNIAKKILDALSDMKTHEFEKYVEFYNEFNRALKEGLYSDHQRKESL
                     RDLMLFESTRTEKGKYTSLKTYVDGMKSDQEFIYYMTGASREELERSPYLEAVKDKDY
                     EVLFLTDEIDEFIVSELEYSGKKLKSLTKGDIELDKDKKESKKEAEKKYGKLIDLLKD
                     QLKEEIKDVRVSARLKDSACCLVADEDGMDQNMER"
     gene            complement(1888..2307)
                     /locus_tag="PK754_03040"
     CDS             complement(1888..2307)
                     /locus_tag="PK754_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004764489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08509.1"
                     /translation="MNVIIRNVFAVIVGAIVGSIVNMSIIMIGGQLIPPPAGADVTTM
                     EGLKSSMHLFELRHFLMPFLAHALGTLGGATVAALIAANHKMKFALSIGFVFLLGGLI
                     NVFMLPSPVWFTVVDLAGAYLPMAYLGGKWMSKNQNI"
     gene            complement(2376..2900)
                     /locus_tag="PK754_03045"
     CDS             complement(2376..2900)
                     /locus_tag="PK754_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009333661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB08510.1"
                     /translation="MNFSLAQSIEILERTPMILTAWLDGLSPEWTSSNEGPDTWSAYD
                     IVGHLIHGEKTDWITRAEIILSDQPDKTFKPFDRFAQFEDSKNKTLRQLLTSFDELRA
                     KNIKKLQSLNISEQNLNRTGIHPAFGTVTLVQLLSTWAVHDLDHISQIARVLAKRYKD
                     DTGPWVAYLKILRQ"
     gene            complement(3077..4417)
                     /locus_tag="PK754_03050"
     CDS             complement(3077..4417)
                     /locus_tag="PK754_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08511.1"
                     /translation="MKKSLLLLLLIGSIQLISCGDDDKKDTPAEVPSAYTSLPGTLIV
                     EDETWSGTKTLTGAYYILPGVTLTIEPGTVIKFTYHNNNPDSVGSIFALKADPTNFST
                     PRASARLEAEGTAANPIIFTSAKTTKAAGDWGGIVLIGEAPNNIPGGIGDVEGVSSEI
                     QYGGSNTADNSGTLKYVRIEYCGFGLITDSELNGLSLYSCGSGTTLDHVQVYKCTDDG
                     FEFFGGSVNAKYLVSAYNDDDSFDHDQGWNGKGQFWLGVQTTGADNGFESDGRHTLGS
                     GNPTKPTIYNVTLYGLGTGKDAADGNRGMRLREDFEGTLKNFLITNFAGVAWRLESAS
                     GDSTSRNYDKGLLTITNAVVYNNDRDVTVAGNYGGFNSLNDSTRFFAAANVTEANPNF
                     TNVGSKDFSVQAGSPCLSNAATPPSDGFFDVSATYRGAMGSTNWATGSWVRWSD"
     gene            complement(4522..7203)
                     /locus_tag="PK754_03055"
     CDS             complement(4522..7203)
                     /locus_tag="PK754_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB08512.1"
                     /translation="MKNRLPRFPLYLAAVLFFVIPTFLQAQVVGKITGTIVDASNGDP
                     LIGANVFVIGTKLGGITDLDGNFIIGKIPVGTYSIEISCLSYKKKIVHNLTVNDGLAS
                     TLKISLEPEALEGEEVVVEAEVLKTQESGLLLAQKKSSKVMDGVSSEQIAKTSDGNAG
                     AALKRVTGVTIVGDKDVYVRGLGNRYSNVQLNGSVLPSTNPNQKETPLNIFSSNVIDN
                     IIVQKTYTPDQPGEFSGGSVQIETKDFPESRTIKIGVSSMFNSNSIGNDYLNYNGGGS
                     DFLGFDDGTRKLPTAAKQGRIGTSQGVTAIRQFQNSWSPRSSRTTPGQSYSISYGDQF
                     AVGSRALGFIAAINYSYQNQVRNEVLRNLQQAQSLASDYDASRGMTSTLLSGMLNVSY
                     KINPTSKVSLRNLYTNVSDNTTSRVEGYYYNSGGDYRQTQLRFSQRSIFASNLQYDTY
                     IKEWMDSKILIEATMAAANAYEPDTRNTHYAYNSTLHQYEIVLDQRTNTHFFSDQKDR
                     DYNVKLNWDLKLLPTLRLKTGGLFYDKQRDFEARRFAIAGDPQSVYPAELKTTNPEEA
                     LNPDLFSNGGLIFFETTRNTDSYRGDQNIYAGYLSADITIGNRWNLIAGARAEQSIQR
                     IDKNEVLNTADLLPAVNLTFKVNDRANLRAAFSATLARPEFRELSNFFYSDFVGARTV
                     YGNPDLKRTKIKNYDLRWETYPGIGEYFAVSGFYKHFTNPIEIFHRLSTNPEVYYGNI
                     AEAELWGVELEARKALTEALRVTANLAVMTSSVNYGKAVYSQANRNRPMYGQSPYTIN
                     LNGFYVLRSATEFSLAYNRFGKRLSSVGNVSQGEDEYEMPFDKLDFTATQKFGKWTYK
                     LSIQNLLNERVINRQGDIITRDYAPGVTYSVGASYDF"
     gene            complement(7427..8428)
                     /locus_tag="PK754_03060"
     CDS             complement(7427..8428)
                     /locus_tag="PK754_03060"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNB08513.1"
                     /translation="MAVADLTRKTILQGDIAQSGMTSILSLIKLSQKSGTLRLKDGEK
                     NASIRIEKNYFIEASAPEEILLGEFLVREGYLTEQDLAKTLPLQKRMHIPLGQLLQGM
                     GYFGLDANALTRILNRYLREVIFRIMKWNRGTFTVEETVKKDFPVREEIPVRLNMDFV
                     LLDTTQRLDEWKRLASSVTSLQSALKINRRAISENASLTLNADQLTVLSYVNGRRTLV
                     RILEKIGHNEMFYLSVIDDLLKRRILIERQAEAMRVIIPGRIKADAVNRNRQFPGKFA
                     ANLLYKEIDGKKNLHELADSLHFELPDLWENISLLLKNEAVEIIEGRREFHNLSEEM"
     gene            complement(8514..9815)
                     /locus_tag="PK754_03065"
     CDS             complement(8514..9815)
                     /locus_tag="PK754_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08514.1"
                     /translation="MIKFLNRVIIIILISYFFIVKDGRSQSLTGNKNPFIARFSSGWN
                     YYSRGASLNPQLSEFTQYAMLNYRTLDDSNRIIWQIRTDIYQKDAVTQTRLRNQNEQF
                     DHRTVIRQAYGSYTLRDGEVKLGRFVPIGTHTDAYPINGLGAENTRITSRWRGSVYGG
                     KIFDEYDNRPEGIGYSTGGSLTYDQTRWQAGAGLSAEQLRNTRLTKAYLFGEYRPSYT
                     WRIVSNNQYVVNKALLGYSQNTLYYRLNKNLSTRFFAEYHDRRAYFPAPTDSLSMDRF
                     FNTTQELMIGGSVRYRIFQWRKIGMLETMPSLKKRLGNDNLTYAALQFFYQNYFFWRF
                     NLNTGFSYTDNQWIRNYRSHLSLNKDFFASRLDASFSLIVNTYTWNSTSSRAKLLTTV
                     SADATYRFDASWYISAGIYEEVGNATDPHSGVNIRMFYYLH"
     gene            complement(9808..10563)
                     /locus_tag="PK754_03070"
     CDS             complement(9808..10563)
                     /locus_tag="PK754_03070"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNB08515.1"
                     /translation="MNRSLIIAAALLSMAAYLPQPDPEYTAWTYARPAADTPYVKIPH
                     KPFDIPCDACHTTTTWKILKPKIEYDHTKTGFPLVGAHLQTACSQCHGGGVFRSAIRE
                     CFVCHTDPHQAQLGLECERCHTEYAWAPSIFKHEDQNLFMFGAHKGLDCTGCHKNLIT
                     FAMPNVQECGDCHIPLGATTDHNAYKSIGDCMACHNMNGWSNYPHADEWFSLTGHHRR
                     SCETCHKQIPNYTTYTCRDCHRFDHKGEGHDDD"
     gene            complement(10560..12323)
                     /locus_tag="PK754_03075"
     CDS             complement(10560..12323)
                     /locus_tag="PK754_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08516.1"
                     /translation="MKHFLHTMLSVMMAWSLHAQFSPGDLASSHKQLEGIQQCTQCHD
                     LGKKIDDARCLDCHTLIKTRIANNKGFHSSAETKNKACIECHHDHLGRDYAMIQWKPS
                     REKFDHTQTGYILDGKHQTTQCEVCHKEAFIKDNAVRARTGDGLLLNKTFLGLSQDCR
                     SCHFDEHRDQFDQCLNCHDFNDWKKSSEKIFDHNKSKYPLTGLHAKVSCEKCHTSVAD
                     PRQKADHKTDETYFKYRPLQFANCTPCHADPHQNRFGQDCQKCHLTASWKQIKNALFD
                     HRQTRYPLEGLHITVRCEKCHQPDSIKPAVYKNMSFSNCTDCHTDAHVGQLTQRSDQG
                     RCETCHNVNGFRPSRFTVSMHATESSYPLIGAHEKTDCNKCHIKANASEFLRRTGIRP
                     RVDSTHIILKFKEKTCQSCHTDIHRGQFADKLKSNDCDICHKTTTWKDIVFDHQKNSD
                     FKLIGKHEKTPCEKCHLLVDVGTEIKRTLYKPIPTYCERCHNDIHEGQFAEYKTMTPS
                     HPKTDCAKCHSNDAFKPALFDHEKQSQFPLTGAHIKVECVKCHIRARITSDTLNVVYK
                     PISTECAACHPDQHEGVFQQR"
     gene            complement(12326..12901)
                     /locus_tag="PK754_03080"
     CDS             complement(12326..12901)
                     /locus_tag="PK754_03080"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB08517.1"
                     /translation="MKKHLPVLLIAILLLAIAAYQTLETQQTTSVKTSSNKPRPTLGL
                     MKDQNPDNNSQVFAIKQLQDYLQGHPDDADHWIQLGNLLFDNGNFADAVKPYTEALRL
                     QPENPDVRTDLGVCLFNTGMNDEAILQFERALQYAPDHITATFNLGIVHTRMGHTPSA
                     VQYWRKTIRLGPDTDMGKKATRALTDAGYKP"
     gene            complement(12918..13796)
                     /locus_tag="PK754_03085"
     CDS             complement(12918..13796)
                     /locus_tag="PK754_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08518.1"
                     /translation="MNKRMHRWYILSMTVAGFIVVLSIGIYGWDYYGTPLPLRHDHVQ
                     HGLLSPTGFWGHGMGFIGAFLMTFGVLLYSLRKRARWMAAVGQIKHILELHIFLCLLG
                     PALIVFHSAFKFGGIIGVSFWCMVIVVASGVIGRYLYLQIPKTITGREITPIELEKQI
                     TTLRDRLQKDDGIEEYLLQRLDALSAAFTETSRLSIIRAFPAMIIQNLRHDARVRTLL
                     QEFEKNRNPFSSDVHTRLTMKAHLQRQLTNLTLTQKMFRYWHMFHLPFAVVMFIIMIA
                     HIVTAFLFGYGWIFTS"
     gene            complement(13793..15124)
                     /locus_tag="PK754_03090"
     CDS             complement(13793..15124)
                     /locus_tag="PK754_03090"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNB08519.1"
                     /translation="MSETILTLIISVSLFALVFIPYLWYTNKKRVRFEAKKREAITLG
                     HDKPVAQHPLIDQSRCIGCAACVIACPEHALGMIDGLAELVYPAKCVGHGVCAEACPV
                     SGIRIVLDPTKSTTELPLLDESFQSNIPNLFLIGELGGMALIRNAIFQGRSVIEYITE
                     NIRNTNSSPASSDIHDVIIVGAGPAGLSAGLTAKKNGLDYVLLEKETQAGGAILSYPR
                     QKLVMTTPVEIPLYGLLKQRELSKENLLSMWNDIIQKTDLAVCCDEKIVNVSCENKIF
                     TVYTSAGHTYRSHHVVLALGRRGTPKKLQIPGEDLPKVAYQLLETVRYKHSRSLVVGA
                     GDSAIEAALALSKQTGAEVTLINRGGEFYRAKPKNQDRIRDAERNQKIKIYYQSHVLQ
                     IDPTSVLVQTSNGQVSVENDFVFIFAGGEMPTPFLKQIGIEFIQKSKVIAA"
     gene            15372..17042
                     /locus_tag="PK754_03095"
     CDS             15372..17042
                     /locus_tag="PK754_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNB08520.1"
                     /translation="MANSLFRTKSIDKIMREAEEGMSDAHTPHLSKTLGVRDLTALGV
                     AAVIGAGIFSTIGAAAYDGGPGVIFLFLITAVTCGFTALCYAEFASRVPVAGSAYTYS
                     YVTFGEVIAWIIGWALILEYAIGNIVVAISWSSYFNNLLEGLGLHLPVWLTVDYQTAR
                     NIYQDALRNNLPTDQLVWTMAPSFAGLKVVMNLPACIIVILITILAYVGIKESKNSAN
                     LMVALKLLVLFVVIAVGVFYINTDNWTPFMPNDFTGVLKGVSAVFFAYIGFDAISTTA
                     EECSNPKRDLPRGMMYSLIICTIIYVITTLVITGMVNYKEFQGVSDPLAYVFEKIQME
                     KLGYIISFSAVVAATSVLLVFQIGQPRIWMSMSRDGLLPKKFAEIHPKYHTPGFATII
                     TGVIVAIPALFIEGGIVTDLTSIGTLFAFVLVCGGVLLLPAKKEQEDNRFHLPYINGK
                     WIVPFLLLVFIYFFSERIGSAMTHIGNEAYQEVLFLIFMVIAVVMAVLTFVRNYSLIP
                     ILGMMCCLYLMIEIPVKSWFVFFIWMAVGLSVYFMYGYKNSHLSESKS"
     gene            17055..17252
                     /locus_tag="PK754_03100"
     CDS             17055..17252
                     /locus_tag="PK754_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08521.1"
                     /translation="MTIISRVSIRHPVKEVWKFFDIPTNLSLWFTGFKRFEPIRGTQS
                     EVGVKAKHMYEERGRTIENNA"
     gene            17585..18082
                     /locus_tag="PK754_03105"
     CDS             17585..18082
                     /locus_tag="PK754_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNB08522.1"
                     /translation="MHRNFLLLWLITWSVMGQVEKVSLPELERRLMLNKDSVLVVNFW
                     ATWCSPCIEELPLFDSLYQNAANYKSEKKSVTVMLVSLDFASQLESKVKPFVIKQRLK
                     APVILLDESDANVYINRMHHDWSGAIPATLLIRPSTGERKLIERKFESYDDLKQSIFH
                     FIERK"
     gene            18086..18691
                     /locus_tag="PK754_03110"
     CDS             18086..18691
                     /locus_tag="PK754_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNB08523.1"
                     /translation="MKRFFWLSLIVMVFRISLFAQGYNVGDKAIDFRLKNIDGKTVSL
                     ADYKDAKGFIVTFTCNHCPYAKAYEERIMALDKKYRPLGYPVLAINPNDPVGYPEDSY
                     ENMIARAKEKGYTFPYLVDETQNIARAYGATRTPHMYVLQKTSSGLEVAYIGAIDENT
                     ENASAAKNKYVEWAVDALLKGQKPSTTFTKAIGCTIKWKKS"
     gene            18766..20343
                     /locus_tag="PK754_03115"
     CDS             18766..20343
                     /locus_tag="PK754_03115"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNB08524.1"
                     /translation="MTYTRVIIFVFLQIISALAWTSVAQTKNMSADTTRLKHHVMTMS
                     HDSMRGRRSGYEGEQKAASYIENQFQNIGLSPFIGTSYLQPFLLPRGRINAASYVIIN
                     EDTLRYLTDFLTYEVPDKRTDIESDMVFLGYGIDGPGKSYDAYGGNDIHGKIVVALQP
                     SLIDRARLGEPLNYDHMQLASWARTYGARGLILLENNPGSNKKRFNSASYSEVYRPFS
                     DNFFWISIQNGYAFIQRYFNITQERIEPVMRLTAPVRINTRIDGYWLKSANVIGKLEG
                     TDLANEYIVVTAHYDHIGIGKKINGDSICNGMLDNASGVATMLEAARMLYSSQTKPRR
                     SVLFVATGAEEMGFIGSQYFADHLPIPQSQIVANINLDITGYYYKSEEPYVTLLGAEY
                     NDMGDFIEDAADESGVRVGSNTIPSFFYRSDAVVFARAGIPSIHIACGLPISEMKSGM
                     EYFMNYYHQTTDELYKAPVSFKIMAGQTETVARSIIKIADHDKRPIMTSELDSEKWMG
                     WREAWGGFRMWVGGFFE"
BASE COUNT         5322 a         4380 c         4989 g         5932 t
ORIGIN      
        1 cgttccatgt tttggtccat gccgtcttcg tcggcaacaa ggcagcaagc cgaatctttg
       61 agtcgtgccg agacccgcac atctttgatc tcttccttca actgatcttt gagcaggtca
      121 atcagcttac cgtatttctt ttcggcttct tttttggatt cttttttatc tttgtcgagt
      181 tcgatgtcgc ctttggtcaa agatttgagt tttttaccgc tgtattcgag ttcggatacg
      241 ataaattcgt caatctcatc ggtcaaaaag agcacttcgt aatccttatc tttgaccgct
      301 tccaaatacg gtgagcgttc gagctcttca cgcgaagcgc ctgtcatgta gtagataaat
      361 tcctgatcgg atttcattcc atctacgtaa gttttgagcg aggtatattt acccttttcg
      421 gtgcgtgtgg attcgaacag catgagatcg cgtaaggatt ctttgcgctg atgatcggaa
      481 tacagtccct ctttgagagc acgattaaat tcgttgtaaa actccacata cttttcaaac
      541 tcgtgcgttt tcatgtcgga aagggcatcg agaatttttt tggcaatgtt tttcttgatg
      601 acttccattt gtttgttggc ttgcaggatt tcgcgcgaga cattgagcgg cagatccgat
      661 gactcaacaa cgcctttgac aaaacgaaga tacgtaggaa tggcctgttc gcaatgatcc
      721 atgatctgaa cgcgtttgac atacagcatc ggtccaattt tgaattcctt ataaaacaga
      781 tcaaacggcg cttttttggg aataaaaagc agcgctgaaa attccgatgt accctcggcg
      841 cgaaaatgga tggttttcag cggttcgccg aaatcatgcg agacgtgttt gtaaaaatcg
      901 ttgtattctt ccgtggtgat ttcactttta tctttgagcc aaatggcttt acgcgaattg
      961 agcgtttctt cttcgatttt tttatctttt tcgacatcca tacaaatcgg atgttcgatg
     1021 tagtcggaat atttacgaac gacactgcgt atttcccgct cttccaggta ctttttaccc
     1081 tcttcgttca taaaaagaat cacgtccgtg ccgcgggttg ctttttcaaa cgattccacc
     1141 gtaaacacac cgtccgcttt ggattcccat cgaatagctt tatcctgcgg ttctccggct
     1201 ttgcgtgaaa tgacgactac acgatcagcg accataaatg aagaataaaa accgacaccg
     1261 aattggccga tcaattcagg gttatctttg acctgttttt ctgtaaggcg ttggacgaag
     1321 tcgcgcgtgc cggaatgtgc gatcgtaccc agagaccgga ttatttcttc ttcggtcata
     1381 ccgataccgt tatcggaaat ggtcagtgtg ccggcttctt tgtcaatttt gagtttgatt
     1441 ttccaatcgg catcaccgcc cgatatttgg ctgtcggtga gggaaaggta gcgcacttta
     1501 tcaatcgcat cggaagcatt tgaaataagt tcacggagga atatttcctt gtgcgagtac
     1561 aaggagtgaa tcatcaaatg aagtaattct ttaacctccg ttttaaattc gagtgtctgc
     1621 gaagacataa aaattcacct cataaaatgt ttgaaaaaat aattttagcg ggggaaaata
     1681 gaaaatggga gggaaaaaat caaataggga ggattctcat ccggttaagg ttttatttcg
     1741 ttttgaaagg acataaatcc gtagaaaaaa aacataaccg ttagggatat actgcttcac
     1801 accccggtta cctttgaaag gttatcgtat cccattaaac gatttgatct tgtctgagaa
     1861 gaaatatttc aacgtatcct gtgaagatca aatattttga ttttttgaca tccatttgcc
     1921 gccgagataa gccatcggca aataggcacc agccaagtcc acgacggtaa accataccgg
     1981 agaaggaagc ataaacacat taatcagacc gccgagcaga aaaacaaaac ctatgctcaa
     2041 ggcaaacttc atcttatggt tggccgctat caaagcggct acggtggcac cgccaagcgt
     2101 tcccagtgca tgcgctaaaa acggcataag aaagtgccgc agttcaaaca aatgcataga
     2161 tgatttgagt ccttccatcg tcgtcacatc ggcacccgca ggcggaggaa tcaactggcc
     2221 gccaatcata atgatactca tattgacaat actgccgacg atcgctccga caatcacagc
     2281 aaaaacattt cgaatgatga cgttcatggg tttttcctcc gaataaaacg gttatttaaa
     2341 aaaatacatt aaatatgctg cgcattatcg ggtgactatt gccgcaggat tttgagatag
     2401 gcaacccatg gcccggtatc gtctttgtag cgtttggcca aaacgcgtgc aatctggctg
     2461 atgtgatcga gatcgtgcac cgcccacgtc gaaaggagct gcaccaaggt gaccgtgcca
     2521 aacgcgggat gaataccggt gcggttcaga ttttgttctg aaatattcag cgattggagt
     2581 tttttaatat tttttgcgcg cagttcgtcg aatgaagtta acagctgccg gagggtttta
     2641 tttttgctgt cttcaaattg tgcaaaacga tcaaacggtt tgaatgtctt atcgggttga
     2701 tctgataaaa tgatttccgc acgtgtgatc cagtcggttt tttctccgtg aatcaggtgc
     2761 ccgacgatgt cgtaagctga ccatgtatcg ggaccctcat tggacgaagt ccattccggt
     2821 gataatccgt caagccatgc tgtcagaatc atcggggtac gttcgaggat ttcgatggat
     2881 tgagccagag aaaaattcat aaccgtgtct cctgatgctg tgacttgggc ttgaaaagat
     2941 aaatacccgt cgtgataatg tcatcataaa aaaggtgatg tttattcaca ataaaaatcc
     3001 cgcgcctgta agaacaaacg cgggactgca tgtatctcca acctaaccat ggagaaggat
     3061 tttcagatca tcgtttttag tccgaccaac gcacccagct acctgtggcc caattggtag
     3121 aacccatcgc accgcggtag gtcgcgctca catcaaagaa tccatcgctc ggtggcgtgg
     3181 cggcattgct gaggcatggc gaaccggctt gcacggagaa atctttggag cctacgttgg
     3241 tgaaattggg attggcttcg gtaacattag ccgcggcgaa aaagcgagtc gaatcgttga
     3301 ggctgttgaa tccgccgtag ttgccggcga cggtcacatc acggtcgtta ttatacacga
     3361 cggcattggt gatcgtgaga aggcctttgt catagttacg tgaggtcgag tcgccgctcg
     3421 cgctctcaag acgccatgcg acgccggcga aattggtgat taagaaattt ttgagcgtac
     3481 cttcaaaatc ctcacgcaaa cgcattccac gatttccatc tgcggcgtct ttgccggttc
     3541 cgagtccgta taatgtcaca ttatagatcg tcggtttggt cggattgccg gagccgagcg
     3601 tatggcgccc gtcggattca aagccattgt ccgcaccggt ggtttgtacg ccaagccaga
     3661 attggccttt accgttccaa ccctgatcgt gatcaaaact gtcatcgtca ttataagccg
     3721 agactaaata ttttgcattg acgctgccgc cgaagaattc aaaaccatcg tcggtgcatt
     3781 tgtaaacctg aacgtgatcc aacgtcgtac cgctgccgca cgaatacaaa ctcaatccgt
     3841 tgagctcgga atccgtgatc aatccgaatc cgcagtattc gatacgtaca tatttgagcg
     3901 taccgctatt atcggctgta ttgctgccgc cgtattggat ttccgacgaa acgccttcga
     3961 catcaccgat accgcccgga atattattag gtgcttcacc gatcagtacg ataccgcccc
     4021 agtcgccggc ggctttagtc gttttggcag atgtaaaaat aatcggattg gctgccgttc
     4081 cttcggcttc aagacgtgcg cttgcacgcg gcgtggaaaa attggtagga tcggctttaa
     4141 gtgcaaagat ggaaccgaca ctgtcgggat tgttgttatg gtatgtaaac ttaatcaccg
     4201 taccgggttc gatagtcagc gtcacgccgg gtaatatata atatgcaccg gtcaatgttt
     4261 tggtgccgct ccatgtttca tcctcaacga tcaatgtgcc gggcagagag gtgtacgccg
     4321 agggcacttc ggccggtgta tcctttttgt catcgtcacc gcatgaaatc agttggatcg
     4381 aaccgatcag cagtaacaaa agcaaactct ttttcatgaa aaactcctta cgcttgttat
     4441 gtagttgttt tggtataaca taatctaaca cgaacatgtt acttcgggat tatgcgaaca
     4501 ttacctttat aacatctgtg atcaaaagtc ataagaggcg ccgacgctat aggtcacgcc
     4561 gggtgcatag tcacgtgtaa tgatatcgcc ctgacgattg atgacgcgct cgttgagtaa
     4621 gttttgtatg gacaatttat acgtccattt tccaaatttt tgggtagccg tgaaatccaa
     4681 tttatcaaac ggcatttcgt attcatcttc gccctggctg acattgccta cggagctgag
     4741 acgttttcca aaacgattat aggccaggct gaattctgtt gcagatcgga gtacataaaa
     4801 accattgagg ttgatcgtat acggcgactg gccgtacatc gggcggttgc gattggcttg
     4861 cgaataaaca gcttttccgt agttgacgga cgaagtcatg acggcgaggt ttgccgttac
     4921 acgcaatgct tcggtcagcg ccttacgtgc ttcgagttcg acgccccaca gttcggcttc
     4981 agcgatattg ccgtagtaca cttccggatt ggtgctgagg cggtgaaaga tttcgatcgg
     5041 attcgtaaag tgtttataaa acccgctcac ggcgaaatat tcgccgattc cgggatacgt
     5101 ttcccagcgc aggtcatagt ttttgatttt ggttcgtttc aaatcagggt taccatacac
     5161 cgtgcgcgct ccgacaaagt ccgaatagaa gaagttggac agttcgcgaa actccggacg
     5221 cgcgagcgtg gccgagaaag cggcgcggag gttagcacga tcgttgactt tgaaggtcag
     5281 gttgaccgcc ggaaggaggt ctgccgtgtt gagcacttcg tttttatcaa tgcgctgaat
     5341 agattgttcg gcgcgagcgc cggcaatcag gttccaacgg tttccgatgg taatatctgc
     5401 ggaaaggtaa cctgcataga tgttttgatc accacggtag gagtccgtat tgcgcgtcgt
     5461 ttcaaagaag attaatccgc cattagaaaa caaatccgga ttcaatgctt cttccgggtt
     5521 ggtcgttttg agttcagcgg gatatacgga ctgcggatca ccggctatag caaagcggcg
     5581 ggcttcgaaa tcacgctgtt tatcatagaa caaaccgccg gttttcagtc gtaatgtcgg
     5641 cagaagtttc aaatcccaat tgagtttgac attatagtcg cggtcttttt ggtcggagaa
     5701 gaagtgggta ttggtgcgtt gatccaatac gatctcgtat tgatggagtg tgctattata
     5761 tgcgtagtgc gtattgcgcg tatcgggttc gtaagcattg gctgcggcca tggtcgcttc
     5821 gatcaggatt ttggaatcca tccattcttt gatgtaggta tcgtattgga gattggaagc
     5881 gaagatggat cgttgtgaaa agcgtaattg ggtttgccgg taatcaccgc ctgaattgta
     5941 gtaatatcct tcgacacggc ttgtagtatt gtcggatacg ttggtgtaca gattgcgcag
     6001 gctgactttg gatgtcggat ttattttgta ggatacattg agcataccgc tgagcagagt
     6061 gctggtcatg cctcgggagg catcatagtc ggaagcgagg gattgtgctt gctgaagatt
     6121 gcgcagtact tcgttgcgca cctgattttg atagctgtag ttgatcgcgg cgataaatcc
     6181 gagcgcacgc gacccgacgg cgaactgatc gccatacgat atactgtagc tttgacccgg
     6241 cgtggtacgt gaagaacgtg gtgaccagga attttggaac tggcggatgg ccgtaacacc
     6301 ttgggatgta ccgatacgcc cttgttttgc ggccgtaggg agttttcgtg tgccgtcgtc
     6361 aaaccccaga aaatccgaac cgccgccgtt ataattcaaa tagtcattac cgatgctatt
     6421 gctgttgaac atacttgata cgccgatttt gatcgtacgg gattccggaa agtctttggt
     6481 ttcgatttgc accgaaccgc cggaaaattc gcccggttga tccggcgtgt aggttttttg
     6541 aacgatgata ttgtctatca cgttggatga aaaaatatta aggggtgttt ctttctgatt
     6601 gggattggtg gacggcagca cggagccatt gagctggaca ttgctatagc ggtttccgag
     6661 gccgcgaaca tagacgtctt tatcgccgac gatcgtcacg ccggtaacac gtttgagcgc
     6721 cgcaccggca ttgccgtccg acgttttggc gatttgttca gaactgacgc cgtccatcac
     6781 tttggaagat tttttctgcg ccagcaaaag acccgattcc tgagttttga gaacttcggc
     6841 ttcaacgaca acttcttctc cttccaatgc ctccggttcc agcgaaattt tgagcgtaga
     6901 agcaaggccg tcattgacgg taagattatg gacgattttc tttttgtagg agaggcatga
     6961 aatttcaata ctgtatgtac cgacgggaat tttaccgatg ataaaattac cgtcgagatc
     7021 ggtgatgccg cccagttttg tgccaatcac gaatacgttt gcgccaatca gcggatcacc
     7081 attggatgca tcaacgatag ttccggtgat tttgccgacc acttgcgcct gcaaaaatgt
     7141 cggaatgaca aaaaataaaa cagcggcaag ataaagagga aaacgcggta aacggttttt
     7201 catacgactt ccttaacgat ggttaggtta aatgcttttc gaaatcgccg aaagataaag
     7261 gaagtgtatt aggagagaat tatcgaagtg ttattgtttg agcttaaaaa ttagcgaaat
     7321 gttatcacga agttggcaag agtattaaaa acctggagtg taatgatttg gtaacacaaa
     7381 tttaataata ccggactaaa gtattctgta gtaagaattg ttggttttac atctcttcgc
     7441 ttagattgtg aaactctcgg cggccttcga tgatttcgac ggcctcgttt ttgagcagta
     7501 gggaaatatt ttcccataga tccgggagtt cgaaatgaag gctatcggct aactcatgaa
     7561 ggttcttttt gccgtcgatt tctttatata aaaggttagc tgcaaatttg ccgggaaact
     7621 ggcggtttcg attgaccgca tcggctttga tgcgtcccgg tatgataacg cgcatcgctt
     7681 cggcctgacg ttcgatcagt atgcggcgtt tgagaagatc gtctattacg gaaaggtaga
     7741 acatttcgtt gtggccgatt ttttccagaa tgcgcacaag cgttcgccgc ccgttgacat
     7801 acgatagcac ggtgagttga tcggcgttta atgtgagcga tgcgttttca gagatggccc
     7861 ggcggttgat ttttagtgct gattgcaacg aggtaacact gctggcaagc cgtttccatt
     7921 catccagtcg ttgggtggta tccaataaaa caaaatccat attaaggcgc accgggattt
     7981 cttctcgcac agggaaatct ttttttacgg tttcttccac agtgaatgtg cctctattcc
     8041 atttcataat tctaaaaatc acttctcgta aataccgatt gaggatccgg gtcaacgcat
     8101 ttgcgtccaa tccaaaatat cccatacctt ggagcaattg gcccaacggg atgtgcatgc
     8161 gcttttgtaa cggcagggtt ttagctaaat cttgttcggt cagatagcct tcgcgcacaa
     8221 gaaattcccc tagcaggatt tcttccggtg cggaggcttc gatgaaataa tttttttcga
     8281 tccggatcga ggcgtttttt tcgccgtcct tgaggcggag tgtgcctgat ttttgcgata
     8341 acttgatgag cgaaagtatg ctcgtcatgc cggattgtgc gatatcgcct tggagtatag
     8401 ttttgcgggt taaatcggca acggccatag tagatttgtt tttttgaaaa aatacaaatc
     8461 gtttattatg cccaaactat cgctttgtta ccaagataat ataaagcccg aggtcaatgc
     8521 agataataaa acatgcggat attaacgccg gaatgcggat cggtggcgtt acccacttct
     8581 tcataaattc ctgcggatat ataccacgat gcgtcaaaac gataggtggc gtcggccgaa
     8641 acggtggtca aaagtttggc gcgcgaggat gtggagttcc acgtataagt attaacgata
     8701 agagagaatg aagcgtccaa acgtgaagca aaaaaatcct tattaagtga aagatgggag
     8761 cgataattac gaatccactg attatccgta tagctgaatc cggtattcag gttgaatcgc
     8821 cagaaaaaat aattttgata aaaaaactga agcgccgcat aagtcagatt gtcattaccg
     8881 agacgctttt tcaacgaagg cattgtttct aacatcccga ttttgcgcca ctggaatatc
     8941 cggtaacgca cagagcctcc gatcatcaat tcctgtgttg tgttgaaaaa gcgatccata
     9001 ctaaggctgt ccgtcggcgc aggaaagtag gcgcgccggt catggtattc cgcaaaaaaa
     9061 cgggtgctta gatttttgtt taaacgataa tacaaggtgt tttgcgaata gccgagaagc
     9121 gctttgttca ctacgtattg attgttggat acgatgcgcc atgtgtagga aggccgatat
     9181 tcaccgaaaa gataagcttt ggtaagacgg gtattgcgta gttgttcagc gctcaatccg
     9241 gctccggctt gccaccgggt ttggtcatac gtcaggcttc cgcccgtact atagccgatg
     9301 ccttcagggc gattgtcgta ttcatcaaaa atttttccgc catacacaga acctctccac
     9361 cgcgaggtga tgcgcgtatt ttcggcgccg agaccgttga tcggataagc gtcggtatga
     9421 gttccgatcg ggacgaagcg tcccagtttg acttcgccat cgcgtaatgt ataggatccg
     9481 taggcttgac gaattaccgt tcggtgatca aactgctcgt tttgatttcg cagacgagtt
     9541 tgtgtcacgg cgtctttttg gtaaatatcg gtacgtattt gccagataat tcgattacta
     9601 tcatccaacg tgcgataatt gagcatcgca tactgtgtaa actcgcttag ctgcggattg
     9661 agcgaagcgc cgcgggaata ataattccat ccggaagaaa atcgtgcaat aaatggattt
     9721 ttgttgcccg tgagagattg cgaccggccg tcttttacaa taaagaagta cgagattaat
     9781 atgataatga tgactcggtt taaaaattta atcatcatcg tgtccttcac ctttatggtc
     9841 aaaacgatga cagtcccggc atgtataggt cgtatagttg ggtatctgct tgtggcatgt
     9901 ctcgcagctt cggcgatgat gcccggtcaa agagaaccat tcgtcggcat gcgggtaatt
     9961 gctccatccg ttcatgttat ggcaggccat gcagtcgccg atggatttgt atgcattatg
    10021 atcggtggtt gcgccgagcg ggatatggca atcgccgcac tcctgcacgt tgggcattgc
    10081 aaaggtgatg aggtttttat ggcaaccggt gcaatctaaa cctttgtgtg cgccgaacat
    10141 gaataaattt tgatcctcat gtttaaaaat cgaaggcgcc cacgcatact ccgtgtggca
    10201 ccgctcacac tccaatccaa gttgtgcctg atgcggatcg gtatggcata cgaagcattc
    10261 gcgaatggcc gaacgaaaca caccgccgcc atggcattgg ctgcatgccg tctgaagatg
    10321 ggcgccaacc agcggaaacc cagttttggt gtggtcgtat tcgatctttg gttttaatat
    10381 tttccatgtc gtcgtggtat gacaagcatc gcacggaatg tcaaacggct tatgcggtat
    10441 ttttacatac ggtgtatccg cggcaggacg tgcgtaagtc cacgccgtat actcgggatc
    10501 cggttgcggt aaataggcgg ccatactcag tagcgctgcc gcgatgataa ggcttcggtt
    10561 catcgttgtt gaaacactcc ttcgtgttga tccggatggc atgccgcgca ctcggtacta
    10621 atgggtttgt agactacatt tagtgtgtcc gacgtaatcc gtgcgcgaat atggcatttg
    10681 acgcattcca ctttgatgtg agcgccggtt agtggaaact gagactgctt ttcgtgatcg
    10741 aaaagtgccg gcttgaaagc gtcgttagaa tggcacttag cgcaatcggt tttcggatgc
    10801 gaaggcgtca tggttttata ttcggcaaac tgaccttcat gaatatcgtt gtggcagcgt
    10861 tcgcagtatg tcggtatcgg tttgtacaat gtgcgtttta tctccgtgcc cacatctacc
    10921 aaaagatggc atttttcaca cggtgttttt tcgtgttttc cgattaattt gaaatcagag
    10981 tttttttgat gatcaaaaac aatgtctttc catgttgtgg ttttgtgaca gatgtcgcaa
    11041 tcgttacttt ttaatttatc tgcaaattgc ccgcggtgga tatccgtatg gcacgattgg
    11101 catgtttttt ctttgaactt caggatgata tgtgtgctgt caacgcgagg tcggatgccg
    11161 gttcgacgta aaaattcaga tgcattggct ttaatatggc atttattaca atctgttttt
    11221 tcatgagcgc cgataagcgg ataggaactc tcggttgcat gcatgctgac cgtaaaacgc
    11281 gacggacgga acccgttgac attatgacac gtttcacaac ggccttgatc ggatcgctga
    11341 gttaattgac cgacatgcgc gtcagtatgg caatcggtac agttagaaaa actcatattt
    11401 ttatagacgg ctggttttat agaatcaggt tgatggcatt tttcgcagcg caccgtgatg
    11461 tgtaggccct ccagagggta acgcgtttgc cggtggtcaa ataaggcgtt tttaatttgt
    11521 ttccacgaag ccgtcaaatg gcatttttga caatcctggc cgaatcggtt ttgatgcgga
    11581 tcggcatggc atggtgtaca attggcaaac tgaagcggcc ggtatttgaa ataggtttca
    11641 tctgttttat gatcggcttt ttgacgcggg tcggcaacag aggtgtggca tttttcacag
    11701 gatactttgg cgtgcaatcc cgtgagcgga tattttgatt tgttgtggtc aaaaattttt
    11761 tccgaagatt ttttccaatc attaaaatcg tggcaattca aacattggtc aaattgatca
    11821 cggtgttcgt caaagtggca tgaacggcaa tcttgcgata aacccaaaaa agttttgttg
    11881 agcagtaagc cgtcaccggt tcgtgcgcgt accgcattat ctttgatgaa ggcctctttg
    11941 tggcaaactt cacattgggt cgtttgatgt tttccgtcga ggatatatcc ggtttgtgtg
    12001 tgatcaaatt tttctctgga tggtttccat tggatcatcg catagtctcg tccgaggtga
    12061 tcatgatggc attcgatgca ggctttgttt ttggtttcag ccgaactgtg aaaaccttta
    12121 ttgtttgcga tgcgggtttt tatcaaggta tggcaatcaa ggcatcgcgc atcatctatc
    12181 tttttaccga ggtcgtggca ttgcgtacat tgttgaatgc cttcgagttg tttgtgggac
    12241 gatgccaggt cgcccggtga aaattgtgca tgaagcgacc acgccatcat caccgataac
    12301 attgtatgta gaaaatgctt catgtttatg gtttatatcc cgcatcggtc agggcacgcg
    12361 ttgctttttt tcccatatcg gtatccggac ctaagcgtat tgtttttctc cagtattgca
    12421 cagcggacgg agtgtgtccc atgcgcgtgt ggactatgcc cagattgaat gtcgccgtga
    12481 tgtggtcagg ggcatattgt aaagcacgtt caaactgtag aatggcttca tcattcatgc
    12541 cggtattaaa taaacacacg ccgaggtcgg ttcggacgtc ggggttttca ggttgtaaac
    12601 gcaaggcttc cgtgtatggt tttacggcat ctgcaaaatt accgttatca aacagtaaat
    12661 tacccaactg aatccaatga tcggcatcgt cagggtgtcc ttgcaaataa tcttgaagtt
    12721 gctttatcgc aaatacctgt gaattattgt cagggttttg gtctttcatt aatcctagag
    12781 taggtcgggg tttattcgat gaagtcttta ccgaagtggt ttgttgtgtt tctaatgttt
    12841 ggtatgccgc tatggccaaa agcagtatag ctattaacaa aaccggtaaa tgttttttca
    12901 tacgataagt ctccggatca tgacgtaaaa atccacccgt agccgaataa aaaagccgtc
    12961 actatatgag cgatcatgat gatgaacatg accacggcaa acggaagatg aaacatgtgc
    13021 cagtaccgaa acattttttg tgtaagcgtg agattggtca gttggcgttg cagatgcgct
    13081 ttcatggtca gtcgtgtatg gacgtctgac gaaaacggat tacggttttt ttcaaactcc
    13141 tgaagtaagg tgcgaacgcg cgcatcatga cgtaagtttt gtataatcat tgccggaaaa
    13201 gcacgaatga tcgagagacg tgaagtttct gtaaatgcgg cggaaagcgc gtctaaacgc
    13261 tgtaaaagat attcttcaat gccgtcatct ttctgcaagc gatcacgcag tgtggtgatt
    13321 tgtttttcca attcgatcgg tgtgatttcc ctacctgtaa tggtttttgg aatttgaaga
    13381 tatagatagc ggccgatcac gccgctcgcg acgacgatca ccatacacca aaaacttacc
    13441 ccgataatac cgccaaactt gaatgcggaa tgaaatacga tcaatgcagg tccgagaagg
    13501 cacaaaaaaa tatgcagttc aagtatatgt ttgatctgcc cgacagcagc catccatcgg
    13561 gctcgctttc gtaaggaata caatagtacg ccgaatgtca taagaaatgc gccgatgaaa
    13621 cccatcccgt gaccccaaaa accggtagga ctcaataatc cgtgttgtac atgatcgtgt
    13681 cgcaaaggaa gcggtgtgcc gtaataatcc cagccgtaaa ttccgatact taaaacaacg
    13741 atgaatcctg caacggtcat cgaaaggatg taccagcgat gcattcgttt attcatgccg
    13801 cgatcacctt ggatttttgg ataaactcga ttccgatttg cttaaggaac ggtgtcggca
    13861 tttcgccacc ggcaaaaata aatacaaaat cattttctac cgatacctgg ccgttcgatg
    13921 tctgaacaag tacggacgtc ggatcaattt gtaaaacatg agattggtaa tagattttta
    13981 ttttttgatt ccgttccgcg tcacggatac gatcttgatt tttaggtttg gcgcgataaa
    14041 attctccgcc gcgattgatc agagtgactt cggcaccggt ttgtttggat aaagcgagcg
    14101 cggcttcgat cgcgctgtcc cccgccccca cgaccaatga acggctatgt ttgtagcgta
    14161 ccgtttcaag aagttggtat gctacttttg gcaaatcttc tccgggaatt tgtagttttt
    14221 tgggcgtacc tcggcgaccg agcgccaaga caacgtggtg ggaccggtat gtatgcccgg
    14281 cggatgtgta caccgtaaaa attttatttt cacagcttac attaactatt ttttcatcac
    14341 agcagacggc taaatcggtt ttttggataa tgtcattcca catggagagc agattttctt
    14401 tggataactc acgctgtttg agtaatccgt aaagcggaat ttccaccggt gtcgtcatca
    14461 caagtttttg acgcggataa ctcaaaatag ctccgcccgc ttgggtttct ttttcgagga
    14521 gcacatagtc taatccgttc tttttggccg tcagtcctgc actgagtcct gcaggaccgg
    14581 cgcctacaat gattacatca tgaatatcgg acgaagcggg tgaagaatta gtatttcgga
    14641 tgttttcggt gatatattcg atcacagaac ggccttgaaa aatggcgttt cgtataagcg
    14701 ccataccgcc taattcaccg atcaaaaaaa gattaggtat gttggattga aacgattcat
    14761 ctaacaaagg caattcagtg gtggatttgg tcgggtcaag tacgatacga ataccggaca
    14821 ccggacacgc ttcggcacaa acgccatggc cgacacattt ggccgggtaa acgagttccg
    14881 ccaaaccatc aatcatgccc agtgcatgtt cggggcaagc gatcacgcag gccgcacaac
    14941 cgatacagcg ggattgatcg atcaagggat gctgagctac cggtttatcg tgtcccagcg
    15001 tgatagcttc cctttttttg gcttcgaaac gcactcgttt tttgttggtg taccaaaggt
    15061 aagggataaa taccaatgca aaaagtgata cactgattat cagcgttaga atcgtttccg
    15121 acatttattt atccaaatac atattcatat gcggattgtt tttaataacc ccgatgacgc
    15181 taaagtgcta aattcaaccg ttttgtcata gttaaaaaac tttaatgtta aaaaaaacgc
    15241 gcacacttaa ttggagatta aacttttaac gatctaatct ctacttgata taggatgata
    15301 aaatatgtac aatgcccaac gcttttttgt aatccaatct caaattctca acatagtgta
    15361 aagtaaatcg catggcaaat tcgcttttca ggacaaaatc tattgataaa atcatgcgcg
    15421 aagccgagga gggtatgtcc gacgcgcata cgccgcatct atccaaaaca ttgggtgtac
    15481 gggatcttac ggcgcttggt gtcgcggctg tgattggcgc cggtattttt tccacgatcg
    15541 gtgcagccgc gtatgacggc ggacccggcg tgattttttt gtttttgatc acggctgtga
    15601 cgtgcggctt taccgcttta tgttatgcgg agtttgcaag ccgcgttccc gtagccggta
    15661 gcgcttatac ttattcgtac gtcacatttg gcgaagttat cgcatggatt atcggctggg
    15721 cgctgatact tgaatacgcc atcggtaata tcgtcgtggc gatttcctgg agtagttatt
    15781 tcaataatct tctggaaggg cttggtttgc atcttccggt gtggcttacg gtggactacc
    15841 agacggcacg taacatttac caggatgcac ttcggaataa cttgccgacc gatcagttgg
    15901 tttggactat ggcaccgagt tttgcaggtc tgaaagtagt gatgaattta ccggcttgta
    15961 taatcgtcat tttaattacg attttggctt atgtcggtat caaagagagt aaaaacagtg
    16021 ccaatctgat ggttgctctc aaattgttgg tgctatttgt cgtgatcgca gtcggagttt
    16081 tttatatcaa tacggataat tggacgccgt tcatgcccaa tgattttacg ggcgtactca
    16141 aaggcgtatc cgccgtgttt tttgcatata tcggttttga tgcgatttcc actacggcgg
    16201 aggaatgttc caatcccaaa cgggatttac cgcgcggtat gatgtattcg ctgatcattt
    16261 gtacgatcat ttatgtgatc acgacattgg tgatcacggg tatggtcaat tacaaagaat
    16321 ttcaaggcgt atcggacccg ctcgcgtatg tgtttgaaaa aatccagatg gaaaaactcg
    16381 ggtatattat ttcgttcagc gccgtcgtgg cggcgacaag tgttcttttg gtgtttcaga
    16441 tcggccagcc gcgcatttgg atgagtatga gtcgcgacgg gctacttcct aaaaaatttg
    16501 ccgagattca tccaaaatac catacacccg gctttgcaac gattatcacc ggtgtgatcg
    16561 tggcgattcc tgcgcttttt atcgaaggcg gcatcgttac cgacctgacg agtataggaa
    16621 cactgttcgc atttgtctta gtatgcggag gcgtattact tttgccggcg aaaaaggaac
    16681 aggaggataa ccgttttcat ttgccttata tcaacggcaa atggattgta ccttttctgt
    16741 tgctggtttt tatatatttc ttctccgaac gtatcggcag cgcgatgaca catatcggca
    16801 acgaagcgta tcaggaagtg ttgttcctta tttttatggt tatcgccgtc gtgatggcgg
    16861 tgcttacgtt tgtgcgaaac tattccctca taccgatact cggcatgatg tgttgtctat
    16921 acctgatgat cgaaataccc gtcaaaagct ggtttgtctt ttttatatgg atggctgtag
    16981 ggctttcggt gtattttatg tacggctata aaaacagtca cctttcggag tccaaatcat
    17041 aaaaggattt tagtgtgacg atcatcagcc gcgtttcgat tcgtcacccg gtaaaagaag
    17101 tgtggaagtt ttttgatatt cccaccaatt tatctctgtg gtttaccgga tttaagcggt
    17161 ttgaaccgat ccgtggtacg cagagcgagg tcggcgtgaa agcaaagcat atgtatgaag
    17221 aacgcggtcg taccatcgaa aataacgctt aaataaaaat atcactgttt tcacggcaat
    17281 cgggatcatc aaagtatgcg aggccgaatt tattgagatc aatgttactc tgactcaaat
    17341 gaccgcgaca gttcacgtaa ccttccgcac cttccttttc aaactgatga tgccgctgat
    17401 gaagggagta tttcaaaaac gtcaagacgg ggatttgatt aaacttaagt cctgcgttga
    17461 agcgctttga gtgattgaaa ctttttcgta acggctaacg taaaaacacc agctcctgta
    17521 taataaattg tcaggcaagg cacatcctgt gtcttgcctg gttttattaa atccctgata
    17581 aaaaatgcat cggaattttc ttttattgtg gttgataact tggtctgtaa tggggcaagt
    17641 ggaaaaagtg tcactgcctg aacttgagcg acgattaatg cttaacaaag acagtgttct
    17701 tgtggtcaat ttttgggcta cgtggtgcag tccttgtatt gaggaattgc cgctgtttga
    17761 ttcgctatac caaaacgctg cgaattataa atctgagaag aaaagcgtaa cggttatgtt
    17821 agtcagcttg gattttgctt cacaactgga gtccaaggta aagccatttg ttatcaaaca
    17881 gcgattgaaa gcgccggtga ttcttctgga cgaatcggat gccaatgtgt atatcaatcg
    17941 gatgcaccac gactggagcg gtgcgatacc ggctacgctg ttgattcgcc cttccaccgg
    18001 tgaacgtaaa ctcatcgagc gaaaatttga atcctatgac gatctaaaac aaagcatatt
    18061 tcattttatt gaaaggaaat gaaccatgaa acgttttttt tggttatccc tcatcgtgat
    18121 ggtttttcgg atttccctgt tcgctcaggg ttataacgtg ggcgataaag cgatagattt
    18181 ccgcctaaag aatatagacg ggaaaacagt ctcgcttgcc gattataaag acgctaaagg
    18241 ttttatcgtt acttttacgt gcaatcattg cccatacgct aaagcttatg aagaacgcat
    18301 tatggcgctg gataaaaaat acagaccgct tggttatccg gtgcttgcga tcaatcctaa
    18361 tgatccggta gggtatcctg aagattcgta tgaaaatatg atcgcccgtg ccaaagaaaa
    18421 aggttatacg tttccatatc tggtagatga aacgcaaaac atagctcgtg cgtatggcgc
    18481 aacgcgtact ccgcacatgt atgttctcca aaaaacatcc tccggattag aagtggctta
    18541 tatcggcgct attgatgaaa acactgaaaa cgcatcggcg gcaaaaaata aatacgtcga
    18601 gtgggccgtg gatgctctac tcaagggcca aaagccgagt acgacattca ccaaagctat
    18661 cggctgtacg atcaagtgga agaaatcgta atcttgctca ataaaatgtt tttacacaac
    18721 ccgcttcaaa aacaagcggg ttgatttttt aaaaacggag aatacatgac gtatacgagg
    18781 gtcatcattt ttgtattttt acaaattatc tcagcattag cttggacatc cgtagctcaa
    18841 accaaaaata tgtcagccga tacgacacga ctgaaacatc acgtaatgac catgtcgcac
    18901 gacagcatgc gtggacgcag atccggttat gagggcgaac aaaaagcagc atcctatatt
    18961 gaaaatcaat tccaaaacat cggtttaagt ccgtttatcg gtacgagcta cttacaaccg
    19021 tttcttctgc cgcgcgggcg tatcaacgcc gcatcgtatg ttattatcaa tgaggatacg
    19081 ctgcgttatc ttaccgattt tttgacctat gaagtgccgg acaaaagaac agacattgag
    19141 agtgacatgg tttttctggg atatggcatt gatgggccgg gaaaatcgta cgatgcatac
    19201 ggtggaaatg atattcacgg taaaatcgtc gttgcgctcc agccgtcgct gattgatcgt
    19261 gcacgtcttg gtgaaccttt aaattatgat cacatgcagt tggcatcttg ggcaagaacc
    19321 tatggtgcac ggggacttat tttgctggaa aacaacccgg ggtcaaataa aaaacgattc
    19381 aattctgcat cgtattcgga ggtgtatcgc cctttttctg ataatttttt ttggatatct
    19441 atacaaaacg gatacgcctt cattcaacgt tacttcaaca taacacaaga acgtattgag
    19501 cctgtgatga ggcttaccgc accggtcaga attaatacgc gaatagacgg atattggctt
    19561 aaaagcgcca atgtgatcgg caaattagaa ggtacggatc tggccaatga atatatcgtc
    19621 gtaacagcgc actatgacca tatcggcatt ggtaaaaaaa taaacggaga ctccatctgt
    19681 aacggtatgt tagacaatgc gtccggagtg gcgactatgt tggaagcggc gcgaatgttg
    19741 tattcatcgc aaacaaaacc ccggcgtagt gtgctttttg ttgcaaccgg cgcggaggaa
    19801 atgggattta tcggatcgca gtattttgcc gatcatctgc cgatacccca atcccaaatt
    19861 gttgccaata tcaatttgga catcacgggg tattattata aatccgaaga accttatgtc
    19921 actttactcg gtgcggaata taatgatatg ggcgatttca tcgaagatgc ggctgatgag
    19981 tccggtgtgc gtgtggggtc caatacaatc ccctcgtttt tttatcgcag cgatgcggtt
    20041 gtgttcgcta gagcaggtat tccgtccatc cacattgcgt gcggtcttcc gatcagcgaa
    20101 atgaaaagcg gtatggaata ctttatgaat tactatcatc aaacaacgga tgaattatac
    20161 aaagcgcccg tgagttttaa aatcatggcc ggtcaaacag aaaccgtggc tcgctcaatc
    20221 atcaaaatag ccgatcatga taaacggcct attatgactt cggaattgga ttcggaaaaa
    20281 tggatgggct ggcgcgaggc gtggggcgga ttccgcatgt gggttggagg gtttttcgaa
    20341 taattaacag atcgcagttg gcccgatatt tttctttttt tcaaggtata ttattatatt
    20401 atacatttta atttaatatg ttaatttaca ttcgtttgat tggcaatcaa ttccgccttt
    20461 tagttaatag tgctattaat tttaactgaa gagataaacc ttgaactcaa tgcatcagac
    20521 catggtttta tggttttgga ttgttgttgc tcatgtatca tgattgatcg taacaatcta
    20581 agcgatatga ttttgctcaa gtttgtaatt tattaatttt ttt
//
LOCUS       DAOQNQ010000025        19955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_940_length_19955_cov_5.391910, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000025 DAOQNQ010000000
VERSION     DAOQNQ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19955
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_940_length_19955_cov_5.391910"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..1612)
                     /locus_tag="PK754_03120"
     CDS             complement(257..1612)
                     /locus_tag="PK754_03120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNB08525.1"
                     /translation="MKKWFTLDRKLSTEVLHLALPVVAGLASITAIGLTDTIMVGRLG
                     ATALAATGQAVMVFWTVNWVLRSVELATQAISARRFGEGQLPACGEVLHHALVFSLTL
                     SFFSMIVLYSMAPRLTRWISANEEVASLATGYIRVLFATLWLSGMFFALRGFFSGIGR
                     TRVFLMSAGILFTANLCGNYMFIYGHWGAPAMGVSGAAVGSALSMICGFSFLILYTFW
                     HRKKTLAAYELFSGAKWRWELVGEIARLAWPNMFRGVMVIGGLLVFYAMVDRVGVAQA
                     AVVNLVINIQSVSFMPGYGFGIAAATMIGHSLGAGNTERAERAGYEAMKLGIIFMGSV
                     GILFLVAPEWVVRVFTDDEEVMKYAIGPLRLVGIVQIVDAAGMVLSTALEGAGQTRWV
                     MMMEILVNWGVFLPLTYLLTFTLNMRNYGPWIAWGVYITLFGVISFIKFNRGSWKHVR
                     L"
     gene            complement(1924..3168)
                     /gene="ftsZ"
                     /locus_tag="PK754_03125"
     CDS             complement(1924..3168)
                     /gene="ftsZ"
                     /locus_tag="PK754_03125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNB08526.1"
                     /translation="MITYEEAREYGAKMRIVGIGGGGCNAVNTMIASTLSGVEFVAIN
                     TDVQALERNQAHHKIQIGKNLTRGLGAGADPELGKRAAEEDMDQIKAILDGTDLVFIT
                     CGMGGGTGTGAAPIVASVAKSMGALTIGIVTKPFNFEKEKRMRQALRGVEELRKNVDT
                     LIVIPNEKILMVMPKNTPFIQAFRTADDVLYRATKGIADLISNTGYVNVDFADVKRVM
                     SGAGDAIMGMGVGSGEGRGEMAVKQAINSPLLEETSIEGATGVLLNLTCPQDFTTEEV
                     DQISNMITGVAGEEADVIWGYSVDENLKNEVHVTVIAAGLNKHRYTKSVAPEMPTSAS
                     EQAQQPMTLRTKKNPHDYYEHLKELKEQKYGKLDQPAYERMKESEPVAEEDDFLSKTG
                     TDAQDDSAIPAFLRNAARQRKM"
     gene            complement(3217..4542)
                     /gene="ftsA"
                     /locus_tag="PK754_03130"
     CDS             complement(3217..4542)
                     /gene="ftsA"
                     /locus_tag="PK754_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNB08527.1"
                     /translation="MKNRIVVGLDIGTTKIAALIAEVGGKEIKIKGVGTCPSVGLRRG
                     MVVNIEHTVESIKKAMQQAELMAGIQAESVYVGIAGDHVRGINSHAVVAVGRDDREIT
                     EDDVRRVIDAAKAINMPMDREIIHVIPQEFIVDKQEGIQNPIGLTGTRLEVEAHVVTG
                     AVASAQNIYRCVERAGYKVKDLVLEPLASSYAVLETDEQELGVVLIDIGGGTTDIAMF
                     FDGSIRNTDMLGIAGQAVTNDVSICLKIPYDKAEEIKKKHGCAMASLVDETESILIPG
                     VGGRDARRENRQFLSKVIEARMMEIFNLVRSVIEKSKLRSMMNAGVVLTGGGSLLEGS
                     AELATEIFQLPVRIGSPRGFGGLMDAANSPIYATGIGLIHYGIQKEKSGDDDMEDEGD
                     ETPPEGPTEGDPGETHTDALEMQSQTGEYQAPLYQKISKKMKRWTEEFF"
     gene            complement(4567..5946)
                     /locus_tag="PK754_03135"
     CDS             complement(4567..5946)
                     /locus_tag="PK754_03135"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNB08528.1"
                     /translation="MIRKKREVAQVDLFVKENILPEEPSFLSKSAETGHTENKETVIK
                     KIQIERTFKNPILMGEFNGTAEHKKDESTENAVREIHKRDEVTAEEISIRASVSQPTE
                     EISLDKVFGRSEQAEADAFPAAAEEVKENSGSYDEIIAIAAEEPQPQAVTLSENSEDE
                     SQEAVEVHTKPGIIPKEAKPKIINRDFSEGFYLTEEARIKAEEAKRARKQERRARRIN
                     KLAVKTVSVLLLLFFVMVLFNWQEWVVNSSLFSLRNVYVQGNLIGSKEDIIKAADLDM
                     GRRLAEIDLTKTADRIKMNPLFEHVTVSRNYPSSVVITVKEREPFAFLPMDELYPVDA
                     NGFVLPKLKARMIYNLPVISGMTGVAQPGKKFKNDRLPLALQFLAKAKEADESLFYDI
                     SEVVVNKKDMTVFLNSLHVAFRIEDEELMRAAVYLSAAAQHFKQEDDAVKGTREVDLR
                     YNGQIFLRN"
     gene            complement(5978..6907)
                     /gene="murB"
                     /locus_tag="PK754_03140"
     CDS             complement(5978..6907)
                     /gene="murB"
                     /locus_tag="PK754_03140"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNB08529.1"
                     /translation="MKTTEDIRSGFRGRWTENVLLSDKTWYKIGGPCIAWCEPDDIED
                     LKYLLRMCKKNEIGFFVHGKGSNLLVSDRGISGIVIDLEKACSFTQFEKTQVSAGAGV
                     YMPKLVLECERQGLAGIEMFAGIPGTVGGAIRMNAGCHGKEMFDVLTEVTTLEDDVLV
                     TRSKSEIRFAYRHVETLEKPDTVIVSGKMVLEYGDKEALAEQRKAYMEKRRLTQPINL
                     PSSGSVFKNPAGHHAARLIEEAGLKGFRIGGASVSEKHANFIVNEGKAKAADVLEIIK
                     HIRQTIAQRNGIRLETEVKFVGFNHEELDGITD"
     gene            complement(6904..8265)
                     /gene="murC"
                     /locus_tag="PK754_03145"
     CDS             complement(6904..8265)
                     /gene="murC"
                     /locus_tag="PK754_03145"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNB08530.1"
                     /translation="MIRPIQHIHFIGIGGIGMSGLAEVVFRQGYKVSGSDKAFNEQTA
                     HLQKLGVTCYEGHRAEQVHADLVVYTAAVHEDNPEIIEARRQHIPMIKRSALLGEFLR
                     GKKGVAVAGTHGKTTTSAMIGMMLEHAGLDPTMFIGGVVRQLGTNAKLSDSAWVVVEA
                     DEYDRSFLELHSHIALINNIESDHLDIYRDLDDITGAFTQFAKQTSFFGAVIGNADDP
                     AVVEVLRQCGKRTMTFGLSEAADLRAANIETKFCELHFDVLLQSKKIERMMLPMSGTH
                     NIKNALACIATGLMCEVPVARIAESLAKFGGTGRRFERLGEKDGVLFIDDYAHHPTEI
                     RATLEGARDGAGGRKIIAVFQPHLFSRTRDYFEDFASAFTAANEVILTEIYPAREMPI
                     PGITGEKLYETTKKNHAHVTFIGDKTKLSEAIRNAARPGDIVITMGAGDITHIGRQMV
                     RGV"
     gene            complement(8262..9374)
                     /gene="murG"
                     /locus_tag="PK754_03150"
     CDS             complement(8262..9374)
                     /gene="murG"
                     /locus_tag="PK754_03150"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNB08531.1"
                     /translation="MAWRVIIAAGGTGGHVFPALAIGEEIRAQKPETEILFIGTNRGL
                     EERVIPAAGFRLKTIVMSGVQRSLHPRDVLRNLVMPFKVLFGLLQSITVMWTFRPHAV
                     IGCGGYVTGPIVLLAALLGKKIILQEQNSRPGRTTRSLARWADAIHLTYEDAQRFFDP
                     KCKLMMSGNPLRKGFVRQDMAAARAALGLASERNTLLVLGGSLGARAVNAALLKIMDT
                     LLQDDNIQVLWQTGKPDYENVRYTMKPYSQVQVHAFIDNMIAAYSAADTVLCRAGAMT
                     LSEITMMGLPAVLVPYPFAADNHQEYNARSLSDRGAAMTLLQTDLNEQLLPALRKMMQ
                     DQAYRRAMAEASYALRQPDAAQNIVRHMFSLEGVRA"
     gene            complement(9374..10663)
                     /gene="ftsW"
                     /locus_tag="PK754_03155"
     CDS             complement(9374..10663)
                     /gene="ftsW"
                     /locus_tag="PK754_03155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNB08532.1"
                     /translation="MTTETLTPRKTIDKGLFLIVLTLMLFGIVMVYSASNVIAEQKFG
                     NSTHFLVNQFLRAMMGIVILMFAAKYDYHNYKRHTMMMLLIAGVLCAVVLTLGQLKGA
                     ARWLQVGGFGIQPSEIAKIALVFYVAGYMDSKGDRIRDFQNGLLPPLIMAGVFAVAII
                     LQPNFSTAAVVMGMVILMLFIGGMDIKHLGMMAAVAIPVALLTVILSPYRRARLMAFL
                     DPTADMQGSGYQIKQSLISFANGGLTGVGVGQGQQKLLFLPEPFTDFIYSIIGEEMGF
                     IGAVLVLGLFIVFLFRAIKTARTAPDMYGFYLATGIIISIVVYALVNAGVAVGVFPTT
                     GLPMPFISYGGTSLLFTCFLVGVLLNISSQTVSKDEAMKATAPEYAMRSRFTENFNVG
                     GEIGEEEEYPTDTTPVPTKKAPAKKAVRTNIVGMDMS"
     gene            complement(10667..12019)
                     /gene="murD"
                     /locus_tag="PK754_03160"
     CDS             complement(10667..12019)
                     /gene="murD"
                     /locus_tag="PK754_03160"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNB08533.1"
                     /translation="MRDVKGKKITVIGAASSGIAAARLLKHKGASVFVSEFASEEKKN
                     EAKVLLQNEHIACEFGGHTERATDCDWAVVSPGVPSDIPVLQTLHARNIPVYSEVEIA
                     SWWIPAPVVAITGSNGKTTTTTLIGELFKHAGRTTVVAGNIGTPLSDCTETLPKDGVA
                     VVEVSSFQAEGFESFHPQVGIMLNLSPDHLDRYSGVEAYYAAKKNMIKNQTASDVLVV
                     NQDDATLMQLIGDSKAQRWAFSLQSIPENGAYVENDTLMVKINGKVEPVIATGDIGII
                     GRHNLYNAMAAILAAKALDVKTSVMAETLRSFRGVEHRLEFVAEKSGVRYYNDSKATN
                     VDSTFVALDSFKKEKVILIAGGKHKGSPYTPLKTLVEARVKSLVLIGQAADIIAGDLG
                     AVTRVQRAASMQEAVEISKQTATPGDVVLLSPACASYDMFKNYEDRGRQFKMAVEGLK
                     "
     gene            complement(12024..13130)
                     /gene="mraY"
                     /locus_tag="PK754_03165"
     CDS             complement(12024..13130)
                     /gene="mraY"
                     /locus_tag="PK754_03165"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNB08534.1"
                     /translation="MFYFLYQIFADSGISGSNLFRYITFRSAMAAVTALVISLFVGPF
                     IIRKLREMQIGEEIRLDGPQSHLSKAGTPTMGGLIILASTLIPTMLWADLSNTYVLLV
                     LLATAWMGVVGFIDDYLKAIKKMKKGLVARYKTAGQISLGLIVGLVMIMTPEFERFAT
                     VTTIPFVKDLNFDFGYFYIPMVIFVITATSNATNLTDGLDGLSIGVVSIAMTAFAVMC
                     YVTGNVNFSDYLNILYIPQASELTIFCTAMVGAGLGFLWYNAYPAQVFMGDTGALAIG
                     SALGTLAVLIKKELLLPIIGAVLVAENISVIAQVMYFKYTKKKYGEGRRIFLMAPLHH
                     HFELKGWHETKVVTRFWIMAILCALLTLTTFKVR"
     gene            complement(13143..14510)
                     /gene="murF"
                     /locus_tag="PK754_03170"
     CDS             complement(13143..14510)
                     /gene="murF"
                     /locus_tag="PK754_03170"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNB08535.1"
                     /translation="MPVFTPADLKAIGECRNILDTWSASGVSTDSRDAQTSQMFFALQ
                     GARFDAHAFVHDVAARGIVCVVTAEAFARYASAWEKLPLVIVQDTLHALQRLAENHRQ
                     KSRAKVIGVTGSNGKTTTKEMLKAVMSKTFITQATEGNLNNEIGVPLTLLKLEADTQV
                     AVIEMGADRPGDIETLCRIIKPDFGIVTNIGKAHIERFGRVEAVAETKTALYRHLINH
                     GVRFVNTDDPFLRDQTELAGDLVTYGVNQSAQFRGTILEVSDKGCISLRIEADKSPAM
                     LIRLGVPGLHHAYNVLAAVAVAKHMPMPDEAIVQALEAYRQPANRVSFKEKNGITVMD
                     DTYNANPNSMRAAMDTLMSIKTSGRKIAVLADMLELGAMADNEHRDMGAYAAEKKVDA
                     LYLTGPLAVHAMDGAQGMKNKFYFEDKARLIEMLKDAVQPGDVVLVKGSRGMKMEDIV
                     QAILA"
     gene            complement(14517..14705)
                     /locus_tag="PK754_03175"
     CDS             complement(14517..14705)
                     /locus_tag="PK754_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08536.1"
                     /translation="MDFEKKKEELGFRHFDPKTFEPGVFSHNELLSDIRNNPDKLDVH
                     SKEYFDETNYKLFPKKDQ"
     gene            complement(14722..16914)
                     /locus_tag="PK754_03180"
     CDS             complement(14722..16914)
                     /locus_tag="PK754_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNB08537.1"
                     /translation="MPKQKNMTPKPEPKIQPKTDHASFVKTRVERLRILAFGLVAFAL
                     VIVGRLFFVQVANIGDYEAMAKRQYERRAVLEAERGVIYDRNMQKIAVNLINYSFAAD
                     PSFMNEQDKARVAENFSRVFGKPKNEYLKRLNKPGAFVWLERRVNDQVAEKVNDSVKG
                     LIKLQSLRRHYPFGKAGAALLGMTNIDNEGSAGIELDQQATLGGKDGWAILQADALGR
                     LYPNPDYPHEDPVNGKDVVLTVDMNYQMAAYQVLEKTVKDYNADNATAVLLSPQTGEI
                     LAMVNYPGLDPNAPETFDAELLRNRAITDLYEPGSAFKAFSAVAALEEKVRNPEDRIN
                     CEWGKMTLYGHVIHDTKKHGTISFTEVVQKSSNIGIIKTTDLIGSDKLYQYVRAFGFG
                     NETGVDLEGETRGLLSHPSTWSGLSRPMISIGQEIGVTPLQMVTAYSAIANGGTLYKP
                     YIIKGVHDPLERTQTEHETQLIRTVASQETMQRVSRMLKEVVEGGTGHRAAIKGIEVA
                     GKTGTAQKIDANTGRYAPNSYVASFAGFFPVQNPQVAFIVTVDNPRRSIWGEASAANA
                     TRQILEKIINSSDDFAKHVNRVLADLDQDTTAGDAAMTAPDVKYLNIAAAKNVLDRMN
                     LEFRIAGTGDMVTTQEWTYDPKLKQNILLLTTGNAVTVSESDTLDFRKKRIPDVRGLS
                     MRMAMNKLYESGIDVKIKGNGYVIAQYPRAGAVAKNGESVIIQCKPNL"
     gene            complement(16886..17437)
                     /gene="ftsL"
                     /locus_tag="PK754_03185"
     CDS             complement(16886..17437)
                     /gene="ftsL"
                     /locus_tag="PK754_03185"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HNB08538.1"
                     /translation="MSTYSGNIVGVDFSGRKKKTTETPVSPAERTKTEDRYARYSKAP
                     VKSSARENETVRVEAKPAVQETVASAAVEASNKKGITPLRMFLAFVVVAILMLVWVWM
                     SVTVRQEMLMLEQLKDKQLETQKANESLQAEITRLSNYERIAKIAKEQLKMKPATEKP
                     GVIFLDTEKQKMLQDAEAKKYDP"
     gene            complement(17464..18387)
                     /gene="rsmH"
                     /locus_tag="PK754_03190"
     CDS             complement(17464..18387)
                     /gene="rsmH"
                     /locus_tag="PK754_03190"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNB08539.1"
                     /translation="MPDYHIPVLLQSCIDFLQIRPDGFYVDGTLGGGGHSAFILQQND
                     SCRLFAFDMDRDAIKAAGIRLAEYGDRATLINDNFRHVRSALAARGITQINGILLDLG
                     VSSHQLDWSDKGFSYRFEAPLTMAMADDTAFTARDVVNGYSEQELARIFFHYGEEQQS
                     RRIARTIVQQRTTTPLQTTKDLADLIRRIIPERHANKTLSRIFQAIRIEVNDELNNLR
                     QALADATGILAIGGRIVVMSYHSLEDRIVKDFFRDEAMDKILNTHYPELSEPKTPRLK
                     VITKKPYEATDEEIQLNPRARSVKLRVAERI"
     gene            complement(18391..18828)
                     /gene="mraZ"
                     /locus_tag="PK754_03195"
     CDS             complement(18391..18828)
                     /gene="mraZ"
                     /locus_tag="PK754_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNB08540.1"
                     /translation="MPSFKGTYNHILDSKNRFNIPAKMRSAFVPEDKDTVILTRGFEQ
                     CIYMYPYSEWLRREDELRQLSNMDSDARKLVRLLMGFAQECELDKQGRVIIPQPLLSF
                     AGIEKEIVIIGALERMEAWNPKQYDAAHNGFDLETTAAKFVKF"
     gene            19318..>19955
                     /locus_tag="PK754_03200"
     CDS             19318..>19955
                     /locus_tag="PK754_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNB08541.1"
                     /translation="MKRFMYDFLQKNAAMLCVLLCCSASLFAQNNALTLNGTSQYAYY
                     NGSLSTYEDVTMEGWFKWDGSTGNNQLLMYDGHTSLNGFGLMLDASASNNLIILAGGY
                     QVTSVGRGLDVGEWSHVALVKKGNFFSLYINGDPEIAKAMTYSYPSGSYMVGGNSINN
                     SEFFKGAIDEVHLWNRALSYNEIFTAAATPMTGAETNLVASYRLDNSADGLTG"
BASE COUNT         4951 a         5362 c         4347 g         5295 t
ORIGIN      
        1 ctgagccagg aagaaggttg agaccagcaa aagcatcgcc gcgaccacgc gatctttggg
       61 tgtccaatac atggcgataa tcgctcccag tggcaacgat gcagcgctca aaagtcccat
      121 cagaaatgat tgaacagcca aatcttgtag catgattttt tatcctcggt taaatagatc
      181 aacgaggggt gtcaaagcga agtgcgatgg atttattgta ctaacaaacg atgaaaagtg
      241 caatcgctgc attcgtttac aaacgcacat gtttccaaga gccgcgattg aattttataa
      301 aactaattac accaaacaat gtgatgtaaa caccccacgc tatccacggc ccgtagtttc
      361 gcatattcag agtaaatgta agcaaataag tcagcggcaa aaaaacaccc cagttaacca
      421 aaatttccat catcatcacc catcgcgttt gccctgcgcc ctcaagcgcc gttgataaaa
      481 ccatcccggc tgcatctacg atttgtacta ttccgacaag ccgcaacggg ccaatcgcat
      541 acttcatcac ttcttcgtca tccgtaaata cacgcacgac ccattccggt gcgacgagaa
      601 atagaatgcc aacgcttccc ataaatataa taccgagttt catcgcttcg taacctgcac
      661 gttcggcgcg ttccgtgtta ccggcgccaa gactgtgacc gatcatcgtc gcggcggcaa
      721 tcccgaaacc atatcccggc ataaatgaaa ccgattgaat attgatcacc aaattaacta
      781 cagcggcttg cgcgacaccg acccgatcca ccatagcata aaacaccaac aatccgccga
      841 tgaccatcac gccacgaaac atattgggcc aagccagccg ggcaatctct ccgaccaatt
      901 cccagcgcca ctttgcaccg gaaaataatt cataagcggc taatgttttt ttacgatgcc
      961 aaaacgtgta taaaataaga aaagaaaacc cgcatatcat ggaaagcgcc gagccgacgg
     1021 ctgcaccgga tacgcccatt gcaggcgcac cccaatggcc atagataaac atataattgc
     1081 cgcacaaatt ggccgtaaat aatatgcccg ccgacataag aaatacccgt gtgcgaccga
     1141 taccgctgaa aaaaccgcgc aatgcaaaga acattcccga caaccaaagc gtcgcaaata
     1201 aaacacggat gtatcccgtc gccaaagagg ctacttcttc attagctgat atccatcgtg
     1261 tcaaccgggg cgccatgcta tacaaaacaa tcattgagaa aaaactcaac gttagtgaaa
     1321 acaccagcgc atgatgcagt acttcgccac aggccgggag ttgcccttca ccaaatcggc
     1381 gcgcactgat cgcttgcgtc gccaattcga cggagcggag aacccaattg acggtccaaa
     1441 acaccataac ggcttgaccg gttgcagcca acgccgttgc gccgagtcgg cccaccatga
     1501 tcgtatccgt cagacctatc gccgtaatgg atgcaagccc tgcaaccacc ggaagtgcca
     1561 gatgcagtac ctctgtagaa agtttacgat ctaatgtgaa ccactttttc ataccgtgta
     1621 taatccgtat taatccgata acaaaatcgt atcaagattt catcactata cgatgaaaat
     1681 ctcttttgtt caatctcgca cacgcaatct tgatcactaa gtttgtctaa gtgatcgtat
     1741 aaaagttgat ccatccttcg tattctaata ttaaaaaatc agaacccgtc ctctgacatt
     1801 agattcaatg aacttcatat acaatccttg cgtgataaaa ttaaacaaat aaaaaagccg
     1861 ctaatggaac attccaataa cggcttaacc ctgtcttttt gagagaaaag acgtttatat
     1921 tttttacatc tttcgttgac gagcggcatt acgcaaaaaa gccggtatag cgctgtcatc
     1981 ctgtgcatcc gtaccggtct tggacagaaa atcgtcttct tccgcaacgg gttcgctctc
     2041 tttcatacgt tcgtatgccg gctgatccag tttaccgtat ttctgctctt tgagttcttt
     2101 caaatgctca taataatcat gcggattttt tttcgtccgc aatgtcatcg gttgctgcgc
     2161 ctgttcagaa gccgaagtcg gcatctccgg ggcaacggat ttggtataac gatgtttatt
     2221 gagaccggcg gcgataaccg tgacgtgtac ttcgtttttg agattttcgt ccaccgaata
     2281 accccagatc acatccgctt cttcgccagc gacaccggtg atcatattgg aaatctgatc
     2341 tacttcttcc gttgtaaaat cctgagggca ggtcagattg agcaatacgc cggttgcacc
     2401 ttcgatactc gtttcttcca ggagagggct attgatggct tgtttgaccg ccatttctcc
     2461 acgaccctcg ccggaaccga cacccatgcc catgatcgca tcgccagcgc cgctcatgac
     2521 gcgcttgaca tcggcaaaat ccacattgac ataaccggta ttggaaatca aatcggcaat
     2581 acctttggtc gcacgataca gtacgtcgtc agcggtacga aatgcctgaa tgaacggtgt
     2641 atttttgggc atgaccatca gaattttttc gttggggatt acgatcagcg tatccacgtt
     2701 tttgcggagt tcttcaacgc cgcgcaacgc ctgacgcata cgtttctctt tttcaaagtt
     2761 aaacggtttt gtcacgatac cgatcgtcag cgcgcccata cttttagcga ctgaagccac
     2821 aatcggtgcg gcgccggtac ccgtaccgcc acccatgccg cacgtaataa atacgaggtc
     2881 cgtgccgtct aaaatcgctt tgatctgatc catgtcttct tcggcggccc gtttgccgag
     2941 ctccggatct gcaccggcac caagaccgcg tgtcagattt tttccgatct ggattttgtg
     3001 gtgcgcttga ttgcgctcca aggcctgcac atcggtattg atcgcgacaa attcgacgcc
     3061 ggacagcgtg gacgcgatca tcgtattgac ggcattacat ccgccgccac cgatgccgac
     3121 gatgcgcatt ttggcaccgt attcgcgggc ttcttcataa gtaatcataa taatcctcca
     3181 tgggagaggt tggtgaaatg agtattatat taatttttaa aaaaattctt ccgtccagcg
     3241 tttcatcttt ttggaaattt tctgatacaa gggcgcctga tattcgccgg tttggctctg
     3301 catttccaaa gcgtccgtat gcgtttcacc cggatcgccc tccgtcggac cttccggagg
     3361 tgtttcgtca ccttcgtctt ccatatcgtc atcgcccgat ttttcttttt ggataccgta
     3421 gtgaatcaat ccgatacctg tcgcatagat cgggctattg gcggcatcca tgagtccgcc
     3481 gaaaccgcgc ggtgaaccga tacgtaccgg caactggaaa atttccgttg ccaattccgc
     3541 cgacccttcc agcagcgaac cgccgcccgt gagtacaaca ccggcattca tcatactgcg
     3601 caacttactt ttttcgataa cggagcgcac cagattaaaa atttccatca tgcgcgcttc
     3661 gatgactttc gagagaaact ggcgattttc acgccgcgca tcgcgcccac cgactcccgg
     3721 tatcagaata ctttccgttt catccacgag cgaagccatt gcgcacccgt gtttcttctt
     3781 tatttcttcc gctttgtcat agggtatttt taaacaaatt gacacatcat tggtcacggc
     3841 ttgtccggca ataccgagca tatccgtatt acgaatgctg ccgtcaaaaa acatcgcgat
     3901 atccgtcgtg ccgccgccga tatcaatcag tacgactccc aactcttgct catccgtttc
     3961 gagtacggcg taacttgacg ccagcggttc taacactaaa tctttgactt tatatcccgc
     4021 gcgttcgaca caacgataaa tattttgcgc cgaagcgacg gcgcccgtca cgacatgcgc
     4081 ttccacttca aggcgcgtac cggtaagacc gatcggattt tgaatacctt cttgtttatc
     4141 aacgataaat tcttgcggga tgacgtgaat gatttcacga tccatcggca tattgatggc
     4201 tttggcggca tcaatcacgc gacgcacatc gtcttcggtg atctcgcgat cgtcgcgacc
     4261 taccgctacg acagcgtggc tattgattcc gcgaacgtga tctccggcta tgccgacgta
     4321 caccgattcc gcctgaatac cggccatgag ttcggcctgc tgcatggctt ttttgatgga
     4381 ttcgacagta tgttcgatat tgaccaccat accgcgccga agtccgaccg acgggcatgt
     4441 accgacacct ttgattttaa tttcttttcc gccgacctcg gcgatcagcg ccgcgatttt
     4501 ggtggtaccg atatcgagac caaccacaat gcgatttttc ataatatcac ctatgtttta
     4561 tctatgctaa ttcctaagaa aaatttgccc gttgtatcgc agatccactt cgcgcgtacc
     4621 cttgaccgcg tcatcttctt gtttgaagtg ctgcgccgcc gctgaaagat aaacggccgc
     4681 acgcataagt tcttcgtcct ctatgcgaaa agcgacatgc agtgaattca aaaataccgt
     4741 catgtctttt ttattgacga ccacttccga tatatcgtaa aacaaagatt catcggcctc
     4801 tttagccttt gcaagaaatt gtaacgccag cggcaagcgg tcatttttaa attttttgcc
     4861 cggttgcgcc acgccggtca taccggaaat cacaggaaga ttgtaaatca tccgcgcttt
     4921 caatttgggc aacacaaatc cgttcgcatc tacaggatac aattcgtcca tcggcaaaaa
     4981 agcaaacggc tcgcgttctt tgaccgtgat cacgacgctg gacggatagt tacggcttac
     5041 cgtcacatgc tcaaaaagcg gattcatttt gatgcgatct gcggttttgg tcagatcgat
     5101 ttccgccaaa cgccgtccca tatccaaatc cgccgccttg ataatatctt ctttactgcc
     5161 gatcagattg ccttgcacgt acacattgcg taacgagaaa agtgacgaat taaccaccca
     5221 ttcctgccag ttaaaaagca ccataacaaa aaacagcagc aacaacaccg aaaccgtttt
     5281 gacggcaagt ttattgattc gacgcgcgcg ccgctcttgt tttcgggcac gtttggcttc
     5341 ctcggctttg atgcgcgctt cttccgtcag ataaaaacct tccgaaaaat cacgattgat
     5401 gatcttcggt ttggcctctt tgggaataat tccgggtttg gtgtgaactt caaccgcttc
     5461 ttgggattca tcctcagaat tttccgacag cgtgaccgcc tgcggttgag gttcttcagc
     5521 ggcaatggct ataatctcat cgtacgatcc ggaattttct ttcacctctt ccgctgccgc
     5581 cgggaaggca tcggcttccg cttgttcgct ccgaccaaac actttatcca ggctgatctc
     5641 ttccgtcggt tggctaaccg atgcacgaat ggatatttct tccgctgtca cttcatcgcg
     5701 tttgtgaatt tcgcgcaccg cgttttcagt agactcgtct tttttatgtt cggcagttcc
     5761 gttaaattcg cccatcagaa tcggattttt aaacgtccgc tcaatttgaa tcttcttgat
     5821 cacggtttct ttgttttcgg tgtgtcctgt ttcggccgat ttagacaaaa acgaaggttc
     5881 ctccggaagg atattttcct tcacaaacaa atcgacctgt gccacttcgc gtttttttcg
     5941 tatcatagtt taggtacgtt tgcgcatcgc tgcgccgtta atccgtaatc ccgtctaatt
     6001 cttcgtgatt aaatccgaca aacttaactt ccgtttccag acgaatacca ttgcgctgtg
     6061 caatcgtttg tcgaatgtgt ttaataattt cgagaacatc ggcagctttc gctttgccct
     6121 cattgactat aaaattggca tgtttttccg atacacttgc cccgccgatt cgaaaccctt
     6181 tgagcccggc ttcttcgatc aatcgcgccg catgatgacc cgccgggttt ttaaaaacac
     6241 tgccgctgct gggcaaattg atcggctgcg tcaggcgcct tttctccata tatgcttttc
     6301 gttgttcagc caacgcttct ttatcaccat actccagaac catcttaccc gatacgatca
     6361 ccgtgtccgg tttttccaat gtctccacat gacggtacgc gaatcgaatt tccgatttac
     6421 tgcgcgtgac taaaacatca tcttcgagag tagttacctc cgtcagtacg tcaaacattt
     6481 ctttaccatg gcaacccgca ttcattcgta tcgcgccacc gacagttccg ggaatccctg
     6541 caaacatttc gattccagcg agcccctgac gttcacactc cagcaccagc ttaggcatat
     6601 atacacccgc gccggcgctt acctgcgttt tttcaaattg tgtaaaagaa catgcttttt
     6661 cgagatctat cacgatccct gaaataccgc gatcgctgac taacagatta ctgcctttgc
     6721 catgcacaaa aaaaccgatt tcgttttttt tgcacatgcg cagcaaatat ttgagatctt
     6781 ctatatcatc cggttcgcac catgctatac atggtccacc gattttgtac caagttttat
     6841 ccgagagtaa aacgttttcc gtccagcgtc cgcgaaatcc ggaacgtata tcctctgtcg
     6901 ttttcataca ccgcgcacca tctgacgccc aatgtgcgtg atgtcacccg cacccatcgt
     6961 gatcacgata tcacccggcc ttgccgcgtt acgaatggcc tctgatagtt tggttttatc
     7021 gccgatgaac gtgacatgcg catgattttt tttcgttgtt tcgtacaatt tctctccggt
     7081 gatgcccggt atcggcattt cacgcgccgg ataaatttcc gtcagaatca cttcgttggc
     7141 tgccgtaaat gccgacgcaa aatcttcaaa ataatcccgt gtgcgcgaaa aaagatgcgg
     7201 ttgaaacacg gcgatgattt ttcgaccgcc ggcaccgtcc cgcgcaccct caagcgttgc
     7261 gcgtatttcc gtcggatgat gcgcataatc atcaataaaa agaaccccgt ctttttcccc
     7321 gagccgctca aatcgccgtc cagtcccgcc aaacttggcc aggctttcag cgatacgcgc
     7381 tacaggtact tcgcacatca atcccgttgc aatacacgcc agtgcattct taatattgtg
     7441 cgtgccgctc atcggcagca tcatgcgttc gatttttttt gattgtaaaa gaacatcaaa
     7501 atgtaattca caaaattttg tttcgatatt tgccgcgcga agatcagccg cttccgacaa
     7561 accgaaagtc atcgtacgtt taccgcactg ccttagcact tccacgacgg ccggatcatc
     7621 cgcattgccg atcaccgcac caaaaaacga agtctgttta gcaaactgcg taaacgcgcc
     7681 cgtgatatcg tccagatcgc ggtagatgtc aaggtgatcc gattcgatat tattgatcaa
     7741 cgcgatatgc gaatgcaatt ctaaaaacga gcgatcatat tcatccgctt caaccaccac
     7801 ccaagccgaa tcggaaagtt tagcattggt gcccagttgc cggactacac cgccaatgaa
     7861 catggttggg tccaatcctg catgctccag catcatgccg atcatggcgc tggtcgtcgt
     7921 tttaccgtgc gttccggcta ccgcgactcc tttttttccg cgaagaaatt cacccaacag
     7981 cgccgacctt ttgatcatcg gaatgtgctg acgcctggct tcgatgatct cgggattatc
     8041 ttcatgcacg gccgccgtgt atactacaag atcggcgtgc acttgttccg cacgatgccc
     8101 ttcataacac gtaacgccta atttctgaag gtgcgccgtt tgttcattga aggccttatc
     8161 cgatccgctc actttataac cctgacggaa taccacttcg gcaagtccgc tcatgccgat
     8221 gcctccgatg ccgatgaagt gaatatgttg tatcggtcga atcacgcacg aactccttct
     8281 agactaaaca tgtgccgtac aatgttttgg gccgcatccg gttggcgcag tgcataactg
     8341 gcttcagcca tcgcacggcg atatgcttga tcttgcatca tctttcgcaa cgccggtaaa
     8401 agttgttcat tcagatcggt ttgcaataac gtcatcgccg cgccgcgatc cgataaggag
     8461 cgcgcattat attcctgatg attgtccgcc gcaaaaggat acggcacaag tacggccggc
     8521 aaacccatca tagtgatctc tgataacgtc atggcacccg cccggcataa aactgtatcc
     8581 gctgccgaat acgcggcgat catattgtca atgaaagcat gtacctgcac ttgcgaataa
     8641 ggcttcatcg tataccgtac attttcgtaa tccggttttc ccgtttgcca aagaacctgt
     8701 atgttatcgt cttgcaataa cgtatccatg attttaagta atgccgcatt taccgcacgg
     8761 gcgccgaggc tcccgcccag tacaagcaat gtgtttcttt cgctggccaa tcccaacgcc
     8821 gcacgggcag ccgccatatc ctgacggaca aatcctttgc gcaaagggtt accgctcatc
     8881 ataagtttgc acttgggatc aaaaaaccgt tgcgcatctt cgtacgtgag atgaatcgca
     8941 tcagcccaac gcgcaagact ccgtgtcgtt cgaccgggac ggctgttttg ttcctgcaaa
     9001 attatttttt taccaagcag cgccgccaaa agcacgatcg gcccggtcac gtaaccgccg
     9061 catccgatca cggcatgcgg acgaaacgtc cacatcacgg ttattgattg aagcagcccg
     9121 aataacactt tgaacggcat taccagattt ctaagaacat cgcgcggatg caaactgcgt
     9181 tgcacgccgc tcattactat cgttttcaat ctaaatcccg cagccggaat gacacgctct
     9241 tccaagccgc gattcgtacc gataaacaaa atttccgtct ccggtttttg cgcccggatt
     9301 tcttcaccga tggccagcgc tggaaatacg tgtccgccgg taccaccggc cgctatgatc
     9361 acacgccacg ccatcaggac atgtccatac cgacgatatt ggtgcgaacg gcttttttcg
     9421 ccggcgcttt tttcgtcggt accggcgtcg tatcggtcgg atattcttcc tcttcgccga
     9481 tttcaccgcc gacattaaaa ttttccgtga atcggcttcg catcgcatat tccggcgccg
     9541 ttgctttcat cgcttcatct ttgctcaccg tttgcgaaga aatatttaac aatacaccca
     9601 cgagaaaaca ggtgaagagc aacgaggtac cgccgtagct gataaagggc atcggtaagc
     9661 ccgtggtcgg aaatacacct accgccacac ccgcattgac cagtgcatac acgacaatgc
     9721 tgatgataat acctgtcgct aaataaaagc cgtacatatc gggcgctgtg cgcgcagttt
     9781 tgatcgcacg gaaaagaaaa acaataaata atccgagtac cagtacggcg ccgatgaagc
     9841 ccatttcttc gccgatgatc gaataaataa aatccgtaaa cggttccggc aaaaacaaca
     9901 acttctgctg accttgccct acgccgaccc ctgtgagccc gccattggca aaactgatga
     9961 gcgattgttt gatctgataa ccgctaccct gcatatcagc cgtcggatca agaaaagcca
    10021 tcaatcgtgc gcggcgatac gggctgagta tgaccgtcag caacgcaacc ggaatcgcca
    10081 cggccgccat cattcccaag tgtttgatat ccatgccccc gataaaaagc atcaggatga
    10141 ccatacccat tacgacggca gccgtactga aattgggttg aagaataatc gcaacggcaa
    10201 atacaccggc catgatgagc ggcggtaaaa gaccgttttg aaaatcacga atgcggtcac
    10261 ctttgctgtc catataacct gcgacataaa acaccagtgc gattttggct atttccgaag
    10321 gttgaatacc gaaccctccg acttgcaacc atcgggcggc tcctttgagt tgaccgagcg
    10381 tgagcaccac ggcgcaaagc acgcccgcga tgagaagcat catcatcgtg tgccgtttgt
    10441 aattatgata atcgtatttg gcggcaaaca tcaggatcac gattcccatc attgcacgca
    10501 aaaactgatt gaccagaaaa tgcgtactgt tgccgaattt ttgttcagca atcacattgc
    10561 tcgcactgta caccatcacg ataccaaaca gcatcagcgt cagcacgatc agaaaaagcc
    10621 ctttgtcaat ggttttgcgg ggcgttaatg tttctgtagt cataatttat ttcaaacctt
    10681 ccacggccat tttaaactga cggccgcgat cttcataatt tttgaacata tcataactgg
    10741 cacaggccgg cgaaagtaaa acaacatcac cgggcgtggc cgtctgtttt gaaatttcca
    10801 ccgcttcttg catggatgcg gcgcgctgca cgcgtgtcac agcgccgaga tcaccggcaa
    10861 taatgtccgc cgcttgcccg atcagtacga ggctcttcac ccgcgcttcc acgagcgttt
    10921 tcaaaggcgt gtagggcgaa cctttatgtt ttccgccggc aatcaaaatt actttttctt
    10981 tcttaaaact atccaatgcg acaaaggttg aatccacgtt cgtagctttg gaatcattgt
    11041 aataacgcac acctgatttt tccgcgacaa attccaaacg atgttctaca ccgcgaaatg
    11101 atcgcagtgt ttcggccatc accgacgtct ttacatcaag tgcttttgcg gccagaatgg
    11161 ctgccatggc attgtacaga ttatgccggc cgataatacc gatatcgccg gtggcgatta
    11221 ccggttcaac ttttccgttt attttcacca ttaacgtatc attttctacg tacgcgccgt
    11281 tttccggaat actttgtaac gaaaacgccc agcgctgagc cttcgaatcg ccgataagct
    11341 gcatgagcgt cgcatcatcc tgattaacca ccagtacatc cgacgccgtt tgatttttga
    11401 tcatattttt tttggccgca taataagcct ctacaccgct ataacgatcc aaatgatcgg
    11461 gcgacaaatt gagcatgatg ccgacttgcg gatgaaagga ttcaaatcct tcggcttgaa
    11521 agctgctcac ttctacaacg gctacaccgt ctttgggcaa tgtctccgtg caatccgaaa
    11581 gcggcgtgcc gatattacct gcgacaacgg tcgtacgacc ggcgtgttta aaaagctcac
    11641 cgatcaatgt cgtcgtcgtc gttttgccgt tactgcctgt gatcgcgacg accggtgctg
    11701 ggatccacca cgatgcaatt tccacttcgc tatacaccgg tatatttcgc gcatgcagtg
    11761 tttgtagaac ggggatgtcg gatggcacgc ccgggctcac gaccgcccaa tcgcaatccg
    11821 tcgcacgttc ggtgtggccg ccaaactcac acgcgatatg ttcgttttgt aaaagaactt
    11881 tggcttcgtt tttcttttcc tccgaagcga attcgctgac aaaaaccgat gcgcctttgt
    11941 gcttaagcaa gcgggcggcg gcaataccgc tggacgccgc gccgatgacc gttatttttt
    12001 ttcctttcac atcccgcata cgctcaacgc actttaaatg tcgtcaacgt gagcagggca
    12061 cataaaatag ccatgatcca aaatcgcgtc accactttag tttcatgcca acctttgagt
    12121 tcaaaatgat ggtgtaacgg cgccatcaaa aaaatccggc gcccttcgcc gtattttttc
    12181 ttcgtgtatt tgaagtacat cacctgcgca atgacggaaa tattttccgc gaccaacacc
    12241 gcgccgatga tcggcaaaag caattctttt ttgatcaaaa cagccaacgt accgagcgca
    12301 ctgccgatgg ccaaagcgcc ggtatcgccc ataaatacct gagcgggata ggcgttgtac
    12361 caaaggaaac ccaaacccgc gcccaccatg gccgtacaga aaatcgtaag ttcgctcgct
    12421 tgcggaatgt acaggatatt gagatagtcg ctgaagttga cattgcccgt cacatagcac
    12481 atcaccgcaa aggccgtcat cgcgatcgaa acgacgccga tcgaaaggcc gtccaacccg
    12541 tccgtaagat tggtggcgtt ggatgtcgcc gtaattacaa atatcaccat cggtatataa
    12601 aaatacccaa aatcaaaatt gagatcttta acaaaaggta tcgttgtcac cgttgcaaaa
    12661 cgctcaaact ccggtgtcat gatcattacc aaaccgacga tcaatccgag tgaaatctga
    12721 cccgccgttt tatagcgcgc gacaagacct tttttcattt ttttaatcgc tttgagataa
    12781 tcatcaataa agcctaccac acccatccac gccgttgcca gcaatacaag taatacatac
    12841 gtattggaca agtccgccca aagcatcgtc ggtatcaatg tggatgctaa aatgatcaga
    12901 cctcccatcg tcggcgtgcc ggctttggat aaatgcgact gcggtccgtc gagacgaatt
    12961 tcctcgccaa tctgcatttc acgcagtttg cgaataataa acggtccgac aaaaagactg
    13021 atgaccaacg ccgtaaccgc cgccatcgcc gaacgaaacg tgatgtaacg gaaaagattg
    13081 gaaccgctga tcccggaatc cgcaaatatt tgatataaaa aataaaacat aaaatttttt
    13141 cgctatgcta aaattgcctg tacaatatct tccattttca taccgcgcga accttttact
    13201 aaaaccacat cgccgggttg cacggcatct ttcaacatct ctatcaatcg ggctttatct
    13261 tcaaaataaa acttgttctt cataccttgt gcgccgtcca ttgcatggac ggccaacgga
    13321 cccgtgagat acaacgcatc cacctttttt tctgcggcat atgcgcccat gtctctgtgc
    13381 tcgttatccg ccatcgcccc aagttccaac atgtccgcca ataccgcgat tttacgcccg
    13441 gacgttttta tactcatcaa tgtgtccatt gccgcacgca tggaattggg attcgcatta
    13501 taggtatcat ccatcacggt aatcccgttt ttctctttaa aacttacacg gttcgccggt
    13561 tgacgatacg cttctaaagc ctgtactatt gcttcatccg gcatcggcat gtgtttggcc
    13621 accgctaccg ctgcaagcac gttataggca tgatgcaaac ccggtacgcc gagccgaata
    13681 agcatcgccg gtgatttgtc tgcttcgata cgcagcgaga tgcacccttt atcgctgacc
    13741 tcaagtatcg ttccacgaaa ctgcgctgat tgattcacgc cgtatgtaac gagatcgccg
    13801 gccaattcag tttgatcgcg taaaaagggg tcgtccgtat ttacaaatcg tacgccatga
    13861 ttgatcaaat gcctgtacaa tgccgttttc gtttccgcta cggcttcgac gcggccaaag
    13921 cgttcgatgt gcgctttacc gatattagtg acaatgccga aatcgggttt tatgatacga
    13981 cacagcgttt cgatatcacc cgggcgatcc gcacccatct cgataactgc aacttgcgta
    14041 tccgcttcca atttcaaaag tgtcaacgga acgccgattt cattattcag gttaccttcg
    14101 gtggcttgcg tgatgaatgt tttgctcatc acggctttga gcatttcttt ggttgtggtt
    14161 tttccgttgg aacctgtaac gccgatcact ttggcacggg acttttggcg atggttttcg
    14221 gccagtcgtt gcaatgcatg cagcgtgtct tgaacgatca ctaacggcaa cttttcccaa
    14281 gccgacgcat aacgcgcaaa agcctccgcc gtcacgacgc atacgatacc acgcgccgcg
    14341 acatcatgga caaatgcgtg cgcatcaaag cgggcacctt gtaatgcaaa gaacatttgg
    14401 gatgtttgtg catcccggct atccgtcgaa acgcccgatg ccgaccacgt gtctaaaata
    14461 tttcggcatt cgccgatggc tttcagatcc gccggtgtga atacgggcat aacgaattac
    14521 tgatcctttt tgggaaacag tttataattg gtttcgtcaa aatattcttt ggaatgcaca
    14581 tccagtttgt cgggattatt gcgaatatcc gacagcaact cgttgtgtga aaaaacaccg
    14641 ggctcaaacg tcttaggatc gaaatggcga aatcccaatt cttctttttt cttttcaaaa
    14701 tccatacgcc tctcccttca cttagaggtt gggtttgcac tgaatgatga ccgattcacc
    14761 gtttttggcg acggcgccgg cacgcgggta ttgagcgatt acgtaaccgt tgccttttat
    14821 tttaacatcg attcccgact cgtataactt attcatcgcc atacgcatac tcaggcctcg
    14881 cacgtcgggg attcgtttct tgcgaaagtc cagcgtatcc gattcactga ccgtaacggc
    14941 atttccggtc gtcaacaaaa gaatgttctg tttcaatttc gggtcgtatg tccattcttg
    15001 tgtcgtcacc atatcgccgg ttcccgcgat acgaaactcg agattcattc gatccaacac
    15061 atttttggct gcagcgatat tgagatattt cacatccggc gccgtcatcg cggcgtcacc
    15121 ggcggtcgta tcctgatcaa gatcggccaa aacacgattg acgtgtttgg caaaatcatc
    15181 cgacgaattg ataatttttt cgagaatctg tctagttgca ttcgctgcgg aggcttcacc
    15241 ccaaatgctt cggcgcgggt tgtccaccgt tacgataaac gcaacctgcg gattttgtac
    15301 gggaaaaaat cccgcaaacg aagccacata ggaattcggc gcataacgtc cggtattcgc
    15361 atctattttt tgggccgttc cggtttttcc ggccacttcg atacctttga tcgccgcccg
    15421 atgaccggtt ccaccctcga cgacttcttt gagcatccgg ctgactcgct gcatggtctc
    15481 ttgcgaagcc accgtacgga tcaactgtgt ttcgtgttcg gtttgtgtcc gctccagcgg
    15541 atcatgcaca cctttgatga tataaggttt gtacagcgta ccgccgttgg ctatggcgct
    15601 gtacgccgtc accatctgca gcggcgtcac cccgatttcc tgaccgatgg aaatcatcgg
    15661 acgcgaaaga cccgaccaag tgctgggatg actcagcaat ccgcgcgtct cgccttcgag
    15721 atctacgccg gtttcgttgc caaatccgaa cgcacgcaca tattgataaa gtttgtccga
    15781 accgatcagg tcggtcgttt tgataatgcc gatattgctg gatttctgaa cgacctcggt
    15841 aaatgaaatc gtgccgtgct ttttggtgtc gtgtatgaca tgtccataca atgtcatttt
    15901 cccccattcg cagtttatcc ggtcttccgg atttcgcact ttttcttcca acgccgctac
    15961 tgcagaaaag gctttgaatg ccgacccggg ttcatacaaa tccgtaatgg cgcgattgcg
    16021 cagcaactcg gcatcaaacg tttccggagc attgggatcc aatccgggat aattgaccat
    16081 tgcaagaatt tcgcctgttt gcggagacaa caggacagcc gtcgcattat cggcgttgta
    16141 gtctttgacg gttttttcga gaacctgata ggcagccatt tgataattca tatccaccgt
    16201 cagcaccaca tctttaccgt tgacgggatc ttcgtgcggg taatcgggat tgggatacaa
    16261 acgcccgaga gcatcggcct gcaagatcgc ccaaccatct ttcccaccga gcgtggcctg
    16321 ctgatccagc tcgataccgg ccgaaccttc gttatcaata ttggtcatgc cgagaagcgc
    16381 cgcgcccgct ttaccaaacg gatagtggcg ccgcagagat tgcaatttga tcaacccttt
    16441 gactgaatca ttgacttttt cagctacctg atcgttgacg cgccgctcca gccaaacaaa
    16501 cgcgccgggt ttattgagtc gttttaaata ttcgttcttc ggtttaccga aaacgcggga
    16561 gaaattttcc gcaacgcgcg ctttgtcctg ttcattcata aaactcggat cggcggcgaa
    16621 ggaatagttg atcagattga cggcgatttt ctgcatgttg cggtcataga tcacaccgcg
    16681 ttcggcttcc aacaccgccc tccgctcata ctgccgcttg gccatcgcct cgtagtcgcc
    16741 gatattggcc acctgtacaa aaaataaacg tcccacgatg accagtgcaa acgccactaa
    16801 gccaaatgcc aaaatacgca gacgttctac gcgcgttttt acaaaagacg cgtgatctgt
    16861 tttaggctgg attttaggtt cgggtttagg ggtcatattt ttttgcttcg gcatcctgaa
    16921 gcatcttttg tttttctgta tctaagaaaa tcacaccggg tttttctgtt gccggtttca
    16981 ttttcagttg ctctttggcg attttggcaa tgcgttcata attagacaac cgcgtgattt
    17041 cggcttgtaa actctcatta gctttttgcg tctcaagctg tttgtctttg agttgttcca
    17101 gcatgagcat ttcctgacgt accgtcacgg acatccatac ccacactaac atgagtatag
    17161 ccactacgac gaaagcgaga aacatccgga gcggtgtgat accttttttg ttggatgcct
    17221 cgaccgccgc cgacgctaca gtttcttgta cggccggttt ggcttcaacg cgcacggttt
    17281 cgttttcacg cgccgacgat tttaccggcg ctttggaata ccgcgcgtaa cggtcttccg
    17341 ttttcgtgcg ctccgccggc gaaaccggtg tttccgtcgt ttttttcttt cggccggaaa
    17401 aatccacgcc gacaatattt ccactgtatg ttgacatgat atcttatttc aaaaattatt
    17461 tttctatatc cgctccgcca cacgcagttt gacactgcgc gcacgcgggt tcagttgtat
    17521 ttcttcgtcc gtcgcttcat aaggtttttt tgtaatgact ttcagtcgcg gcgttttggg
    17581 ttcggacagt tcggggtagt gcgtattgag tattttatcc atcgcttcat ctctgaaaaa
    17641 atctttgacg atacgatctt ctaacgaatg gtaactcatc accacgatac gtccgccgat
    17701 ggcgagtatc cccgtcgcat cggctaacgc ctgacgcaga ttattcagtt cgtcattgac
    17761 ctcgatacgg atcgcctgaa aaatgcgtga cagcgtttta ttggcgtgtc gctccggtat
    17821 gatgcgtcgt atcaagtcgg ctaaatcttt ggtcgtctga agcggggtcg tcgttcgctg
    17881 ttgtacgatc gtacgcgcga tacgccggga ttgctgctct tcgccgtaat gaaaaaaaat
    17941 acgggcgagc tcttgttcag aatatccgtt gactacgtcg cgcgccgtga atgcggtgtc
    18001 atcggccata gccatcgtga gcggcgcttc gaaccgatag ctgaatcctt tgtcggacca
    18061 atccagctga tgcgaagaaa cgccaaggtc aagcaggatg ccattgatct gtgtgatacc
    18121 ccgagccgct aacgccgaac gcacatgacg aaaattatca ttgatgagcg tcgcgcgatc
    18181 accgtattcc gcaaggcgga tacccgccgc ttttatggca tcgcggtcca tgtcaaatgc
    18241 aaacaaacga caggaatcgt tttgctgaag aataaaagcg ctgtgccctc cgccgcccag
    18301 tgtaccgtca acgtagaacc cgtcgggacg aatctgaaga aaatcaatac aggattgtaa
    18361 aagaacaggt atgtgataat ccggcatgga ttaaaacttt acaaatttcg cggcggtcgt
    18421 ttcgagatca aacccgttgt gcgccgcatc atactgtttg ggattccacg cttccatacg
    18481 ttcgagcgcg ccgatgatga cgatttcctt ttcgatccct gcaaatgaaa gtagcggttg
    18541 gggaatgatc acacggcctt gtttatcaag ttcgcactct tgtgcaaaac ccatcaacaa
    18601 acgaacgagt tttcgcgcgt cggaatccat gttggataat tgacgtaact catcctcacg
    18661 gcgcaaccat tccgaatacg gatacatgta aatacactgc tcaaacccgc gtgtaaggat
    18721 caccgtgtct ttatcctcgg gaacaaacgc gctacgcatc ttcgccggaa tgttgaagcg
    18781 gtttttagaa tccaatatgt ggttgtacgt gcctttaaac gaaggcatac actttaaacc
    18841 ccgttattcc ttaataaacc tttttaaccc actataaccc actttcctac taaattcaac
    18901 taaaacaagc tcaatgtcaa gcgaattttt gttatttctt acactccgga tattgacgct
    18961 tcacaaaaaa tgtgacctgc gatacagaca ttaagagtgg aatttctttc atttcaaggt
    19021 tccatgatca gacctgttta aaagggtata cttcagaaaa atcaaatgat gataaagttc
    19081 cgctgattac atatcgcttg ttgtgtgttt taaaagcaaa aaaacgtata gagatattca
    19141 aattttttac aaactatttt cagttattac gctcttaaat agagatcagc acacatcaaa
    19201 attagatata tgatttgttg acatgctagc tctgttgaag tatattttag ggtaatttct
    19261 cctcatgaaa attcgtaata aattcattct tacttataaa caaaccggag tcacacgatg
    19321 aaacggttca tgtacgattt tctccagaaa aacgcggcga tgttatgcgt tttattgtgc
    19381 tgtagcgcga gtcttttcgc tcagaacaat gctttgacac tcaacggtac gtcgcagtat
    19441 gcttactata acggatcgtt atctacttac gaagacgtta ccatggaagg ttggttcaag
    19501 tgggacggga gcaccggaaa caatcagttg ctgatgtacg acggacacac ttcgctcaac
    19561 ggctttgggc ttatgcttga tgcatctgcc agcaacaatc tgatcattct tgccggtggt
    19621 tatcaggtca cttcggtggg tcgcggatta gatgtcggcg aatggtcgca cgtcgccctc
    19681 gttaagaaag ggaacttttt tagtctttat atcaatggcg acccggagat cgccaaggct
    19741 atgacttact catatccttc cggctcctat atggtcggcg gcaactcgat caataattcg
    19801 gaatttttta aaggcgccat tgacgaagta cacctgtgga atagagcgtt atcttacaat
    19861 gaaattttta cggcggcagc cacgcccatg accggggcgg agacaaactt agtagcgtcc
    19921 taccgtttag acaatagcgc cgatggttta acggg
//
LOCUS       DAOQNQ010000026        19852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_945_length_19852_cov_5.388190, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000026 DAOQNQ010000000
VERSION     DAOQNQ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19852
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_945_length_19852_cov_5.388190"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="PK754_03205"
     CDS             complement(<1..162)
                     /locus_tag="PK754_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNB08542.1"
                     /translation="MSVAEKLNVPAYVPYKVKDMSLAEWGRKEIKLAEAEMPGLMALR
                     AKYGVTKPLK"
     gene            complement(244..726)
                     /locus_tag="PK754_03210"
     CDS             complement(244..726)
                     /locus_tag="PK754_03210"
                     /inference="COORDINATES: protein motif:HMM:NF025734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HNB08543.1"
                     /translation="MKRVLKWLAIGLPVLFIAIQFIRPERTNPPIDPERTLEKHVTIP
                     DDIKTMIKTACNDCHSHETVWPWYSEIAPISWLVAHDVNEGRRHLNFSDWKKYNAHRQ
                     VEKLNEMAGEVRSEKMPMPIYLITHGDARWTKEDRKKFQNWAEDAADALSENLPDEDK
                     "
     gene            852..2624
                     /locus_tag="PK754_03215"
     CDS             852..2624
                     /locus_tag="PK754_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HNB08544.1"
                     /translation="MKRFFITAFLFTSLLSCGKTLQEEADAFLKSYNQEYQAVYYEAS
                     KAQWKALTDINALNDSLSVLANKKYAAFAGSKDIIQKVRFFREHRSELNDITIRQLDK
                     IWLDASHQPGTMPDVVDQLIKANTEATSALYSYQFTVNGKPYSANDIDRALRNSSDLK
                     EREAVWTASKEVGKIERDHLARLQELRNKVAREMKYSSFFDLEAHDYGMSSDEVIQLM
                     DQFSEDLKPLYRELHTYIRYELAKKYNQPVPEYLPAHWLPNRWAQNWPGLVPGLQGEG
                     DIPGKSKEWVVEQAEKFYMSMGFEKLNDNFYKNSDLYPADAASGRKKNTHASAWHLNL
                     DQDYRSLMSVEPNMEWFKTTHHELGHIYYYIAYTNPEVPLTLRAGANRAYHEAMGDLM
                     AVAASQEAYLASLGLFDKTKTPDAIKLLLNSALSNSSIVFLPFAAGTMTHFEYELYEK
                     NLPKEEFNKTWWVLAKKYQGIVPPKERGAEYCDAATKTHIIDDPAQYYDYAISCVLKF
                     QMHDHIAKQILKQDPANCNYYGSKEAGEFIRSIMRPGASKDWRQVLKEKTGQDLSAKA
                     MLDYYGPLYDWLLQQNKGRKKAEF"
     gene            2751..3209
                     /locus_tag="PK754_03220"
     CDS             2751..3209
                     /locus_tag="PK754_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNB08545.1"
                     /translation="MMKGIAELSGYPVVYSTPVAWGEMDALGHVNNIMYFRYFESARS
                     DYFQRIGVWDHAREHGVGVILHSTQCRFKRPLTYPDQIHVGATTVEVMADRFRMKYAV
                     WSEAQQAIVAEGEGLIVVYDYNQNQKCPMPSWMREAIAELEKKASQPTNL"
     gene            complement(3191..3877)
                     /locus_tag="PK754_03225"
     CDS             complement(3191..3877)
                     /locus_tag="PK754_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB08546.1"
                     /translation="MKKLLLVEDEPGLIITLSDRLRREGFVVDIAENGTIALSKARKN
                     RYDIILLDVMLPQSSGLDVCRDLRGDGDRTPILMLTAKSQTTDKVIGLKLGADDYLTK
                     PFDMSELTARIEALLRRSESANRMEASDNYSFNNIEVDFRKAEVRVHKKTVELSAQEF
                     RLLRYMIEHRNATLSRDELLENVWNYGEDITTRTVDVHMAWLRQKLEPNPKRPQYFIT
                     VHRLGYKFVG"
     gene            complement(3874..5655)
                     /locus_tag="PK754_03230"
     CDS             complement(3874..5655)
                     /locus_tag="PK754_03230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB08547.1"
                     /translation="MAKSKRLHPGWIIALLTIALISIGTMQYIWLGRLSEGELTRMRS
                     NLHISATRMASDFDSEISRLHEYLFRVDFDQPEQLPNILQTTVNKWQTNAARQDLIRS
                     VFYGRMSENNTPEVFLLDSARPELHRVSDTVSNPIVLKLQRMRAMRSVGKEKNNLIIM
                     QGHLLADYPYIIVTVNPMRKMLNLPWEQPAEEIIAVLLDTNAVKSWMDSLAGIHFGAS
                     FENDVDIAVINDDQSKFFYKSNSNLPIAHWSKSDISAEIARIRTQDMFMVRVKGTRDK
                     TTMKTLTVTQSIETSERTSNGAFNPIIDSIVTQLHADTKTSGCNLRVIHREGSLEDAV
                     KIVRWRNLFLSAGVLLIMASGLVILLIAQRKERILANQQLEFVAGVSHELKTPLAVIR
                     SAGENLSDAIVTDADDVRRYGELIRGESKRLSDMVDQILDFSGIITDKNSLKMERLDL
                     SIIIQKCIASFPNDTPIYFEDRQTTHNMIIGNHEALQTIFNNLIANGIKYNRANAPIR
                     ITLTDESPYIRTDITDCGIGIDKKDIPHIFEPFFRASGVRDAQIHGNGLGLSIVERYV
                     ALHSGTIRVESEPGKGSTFSVFLPEGT"
     gene            5822..6256
                     /locus_tag="PK754_03235"
     CDS             5822..6256
                     /locus_tag="PK754_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNB08548.1"
                     /translation="MKKIILILGMTLFGVGGNAFAQGIRYMATGPVQPVDEVGVIFAE
                     KENGLIADLIFPEHALPKGYKPQGIEKNDVLVMVNGVSVKTANELKAAYDKVKVGDTI
                     KLGLKRGDEKMIASFAKIDPEHLPKRKMIKRTPDGKEIEETK"
     gene            6256..7746
                     /locus_tag="PK754_03240"
     CDS             6256..7746
                     /locus_tag="PK754_03240"
                     /inference="COORDINATES: protein motif:HMM:NF022100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HNB08549.1"
                     /translation="MLRWFYFTILFFCFTDSGLMAQYNAFNQRDDKYPLLGLKRAKEV
                     YESEKAEYERVKQLFDKQLASPAELERARRSLADVEVNYQQSLLAVLFEQQFITVSRA
                     VKYQDKRGQKRVRVELMNTSGGEAEFRKLISMEDELFRSLQPDHINNIYVSVTNTEGA
                     IISQPYESKVNELVYGQPVTLDFVMLQDLDVVSVNIIYGNGASRSMRIFLQKDATENK
                     VLFQSEQFSQEADLGSNATFDLTLELFSGTDNTFKLEVLNLPPELTRYFLDAATNAKL
                     SQLKFAESARTRKASLRVFLPDRPTEQVQIEKTIPFYVLAIPQEKQALIQTQRDKVWS
                     QQEIESLNAGYLRLELIVKGNARLLVKAPKLYYATTESEPVEASLEIRNDGSRRLDNI
                     KFEVDLPLEWKKEIQPLMIPSIHVNEESVVRFKFYPPEHVAVGKYEVRIRTSSVSDNQ
                     PVNAEDKVITIDIQAGSNALGTMALIVLILSIIGGIVWFGIKLTRK"
     gene            7755..8504
                     /locus_tag="PK754_03245"
     CDS             7755..8504
                     /locus_tag="PK754_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB08550.1"
                     /translation="MTVHNQPVLEAAQLTKRYDDGALALNGISFQVHPGEIYAMLGGN
                     GAGKTTTINIFFNFITPTSGEAKVKGIITHQEPLRAKEQMAFVSENVMLYPNFTAMEN
                     LEFFAKLGGRADITESEMKVTLSRVGLQSEAMNKKVRTFSKGMRQKCGIAIAIIKKAP
                     AIILDEPTSGLDPKAGYEFNRLLIELKQEGKAILMSTHDIFRAKEVADHIGIMNRGSI
                     VMQKNRDELAHEDLEKLYIEYMAGYMEPVAV"
     gene            8528..9910
                     /locus_tag="PK754_03250"
     CDS             8528..9910
                     /locus_tag="PK754_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNB08551.1"
                     /translation="MIKLFFMVWFISSLTVAQSRDVQVSGLDSLIHSAVRDGFHGLVW
                     IESNGKPIYYKGFGFANESNNQKFDSTVFVQIGSTVKDFTRVAIYQLIEKKLLSLDDP
                     LSKYISDLAGEKNKITVQHLLNHTAGFPIGIKFDAEPLTKEEMVEHIRGLNLLSSPGV
                     HEKYSNLGYSCLAYVIEKVSGRSFDAYVDEYILKPLGMHHTGTYRPNFDRNKVAHGYR
                     QNADIGIILDMPHDQDGHLWSLRGNGGYLSTFRDMKLFFDSFETGKLLSTPAFRMAVY
                     DSKQPTVLAGSDMISFYGFANFPGIKARLMIASNHDNYMGHQLLESIEGYLRNGKNPM
                     GKVIKTVEVDNPGESAKVFDLPKEGAGLTIRRYVEAFNSGNSNTMKAFFENYAKSGDG
                     FAPMERRLQNYNRLFSDLGVITVNSFQTRADGSWEVHVGSAKAKAIFNFQIETKAPWR
                     FEALQIELGD"
     gene            9936..11348
                     /locus_tag="PK754_03255"
     CDS             9936..11348
                     /locus_tag="PK754_03255"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB08552.1"
                     /translation="MFTLLLKKEIRDILNSPKFSALFGMGSLLILLAFYMGARNYQVL
                     QNQYESAVRENLRQLEGVTDWSRVEPQIHLPPSPLASLISGVSNDIGRSVQVQAGAAE
                     LVPDHTRYGEDPVYAAFRLLDLEFVFQLILSLVAILLAYNAVNGEREQGTLKLMMAHA
                     VPKDKVIFAKLVGSFTATALPLLVPILIGCGILMVMNVPLTGAEWLRLMLIVCSGMLY
                     YFVFLSVSILVSSLTARSAHAFLALLIIWIFTVLIVPRTAVLLAGRAIEVPSVDEINS
                     QKFRHQSQLFNEHFQKIGEFKPNSNDPEKAMNEFNEFMDKARADREKKVVEFNERLNE
                     DRDNRQKIQQQWAFALGRISPATSFSLAAMQLAGTSSELRNRFEESVKSYKQTFKQFV
                     FEKTGKAPGGNMMMIRIGDSPEEKPKPINPYDLPQFVMPSLTFSEIINAALPDMAILI
                     VINMLIFAATTAAFLRYDVR"
     gene            11361..12824
                     /locus_tag="PK754_03260"
     CDS             11361..12824
                     /locus_tag="PK754_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNB08553.1"
                     /translation="MLSLLIIKEFKNILQSPKFIATFISCTLLIILSVWVGIREYKLA
                     VKDYETGIALNRQDLSERTNWMNVGNRVYRKPDPMQIWTFGVHYDIGRVAHVNTWDSP
                     ELRSSIYSRDLVYAVFRFLDFNFIVVVVLSLFAIVFTYDAVSGEKESGMLKLTFSNGV
                     PKSTYIVAKIIGSWAGLGIPLIVPMLLSFLMTILFQIPLTPDHYARLGGLILAGALYF
                     TFFIILGILISSWTSHSNASFLTLLVLWVTITFVVPRLGVMLSGQVLPVPTAAEVDSQ
                     IEGFSKSQFETLRQNLSETWMKRNNDVQQQPESERQTYRDKMEWTWLEEDQNARKSVQ
                     ENIESFTRKVHEDMDNRKREQIKLGLIFARWSPTSLFQLAAMRFSQTDIFLKERYDDA
                     IRTYQKDFSDVVEKKRRENRAEGLRIEVDSDRGFRVQTPDLKKTIDVRSIPEFTHPAV
                     SAGHAWRLSVWDIVLLAVITGFVFLGALVRFLRYDVR"
     gene            complement(12829..13977)
                     /gene="moeB"
                     /locus_tag="PK754_03265"
     CDS             complement(12829..13977)
                     /gene="moeB"
                     /locus_tag="PK754_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNB08554.1"
                     /translation="MYGLTTEELARYNRHLILKEFGLEGQQKLKSAKVLVIGAGGLGA
                     PCLLYLTAAGVGTIGIVDFDTVDASNLQRQVLYTVDDVGKPKADTAAHRLKQLNPSVH
                     FNTHREKLTSANALDIIRSYDIIADGSDNFQTRYLVNDACVLLNKPLIYASIFQFEGQ
                     VSVFNYTDPSGTLGPNYRDLFPTPPPPGMVPSCAEGGVLGVLPGIIGSMQASEAIKVI
                     TGIGTPLSGKLFLWNALTFESRTMKFKRDPANPINGLQQTITALIDYEVFCGISEPEK
                     QTGGEISPAELKHWIDTGKDFQLIDVRESFEYGICNLGGTLIPLAQIMQESARIAQNK
                     PVVVHCKMGGRSAKAIAQLRTLGFDNLINLRGGIIAYAETVDPSLAKY"
     gene            complement(13990..15276)
                     /locus_tag="PK754_03270"
     CDS             complement(13990..15276)
                     /locus_tag="PK754_03270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNB08555.1"
                     /translation="MKKVVIVDGVRTPYIKAGTLFNKMSAVDLGKLAMRELMARTNLD
                     SKLVDEVIVGNIGNPPEATNVARIIALEAGVPKHVPSFTVGRNCASGMQSIADAYLRI
                     IAGEADVIIAGGGESMTKVPLLFSERATNKFAAMMQARSMPARIGAMTSFRPGDFKPV
                     IGLLLGLTDGFCGLNMGETAEVLAKEYGITRKEQDELALLSHQRATTATDNGILKEEI
                     MPVYLPPDFKKIADEDNGIRKNQTMEALGKLRPAFDKKYGTVTAGNSSQITDGAAFVL
                     VMSEEKAKALGYTPLGSIRGFGFAGLDPERMGLGPVYATPVALKKAGLSMKDIQRIEM
                     NEAFAAQVIANERCFASKKFAEEKLGLTEAVGEIDRQILNVNGGAIALGHPVGSSATR
                     LVLTVLKELKRKNLQFGLATLCIGGGQGGAMIVEHS"
     gene            complement(15284..16789)
                     /locus_tag="PK754_03275"
     CDS             complement(15284..16789)
                     /locus_tag="PK754_03275"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB08556.1"
                     /translation="MNISSQTNSEQFKKEFEEFLKEKYGTADPEQDSAGTKNQGSTLK
                     SGEKTNFDLKPEELEEYLNQFVECQEEAIEVIATKIATHYNRMKLEDTLTDEQKLVGN
                     IKSNVLLIGPTGVGKTYIVKLIAKKIGVPFVKGDATKFSETGYVGGDVEDLVRDLVRE
                     ADGDIKSAEYGIIYLDEIDKIASAQNNRGIDVSRTGVQRNLLKLMEESEVDLRVPHDM
                     AAQMEAAMEAQRTGKVQRKKVNTKNILFVMSGAFGGIEENIRKRINQVRIGFGSDQEG
                     ISKLSRGELMKRVKTEDLVEYGFESEFVGRLPVIAVLNELTVDALFNILKNPNSSVIL
                     SKKRDFAAYGIEVEFDDDALHIFAERAFIQKTGARGLVNILENVLIKFEKKLPSTNIR
                     KFRVTKDVVLQPQAELNKLLTYSSMKEFQQQYLTTHGIIFEFSDVAIQKIAQRSQTEK
                     RSFRAIAAELFKDYEYGLKLAGKTHFLVTPEVFDDPRKYLDELIKKSYSNK"
     gene            complement(16975..17457)
                     /locus_tag="PK754_03280"
     CDS             complement(16975..17457)
                     /locus_tag="PK754_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1203 domain-containing protein"
                     /protein_id="HNB08557.1"
                     /translation="MTKFKIVPLPLNLAERIRTDRMDDQGHTVVEQIASGYGPCRVSL
                     KPFKPGTDRRLLFSHSPFEVDNAFNQSGPVFIHAESVTPYEDIHRFPPEIKADKKHFP
                     LTLIGYSDDQRMVYTRLVGDRDVDQMISDIFEKHTDVAYLHARNAEAGCYICRIDRAE
                     "
     gene            17663..>19852
                     /locus_tag="PK754_03285"
     CDS             17663..>19852
                     /locus_tag="PK754_03285"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNB08558.1"
                     /translation="MKRFLCFAMCGLLCFTLKNVSAQQTWPVEGLHDKTPSIHAFVGA
                     TIIPGPGQKIDNGTIVIRDGVIEAIGKSVNIPREAVIHDLKGMWIYPGLIDAYAGYGI
                     APPAKEKGSDGPQPYTSKKGAYSWNQAVKPEFEAAREFTIDAKRAGELRALGFTTVHA
                     RPLDGIVRGTGAIVNLGESSASEEILSRQATANFSFDKGVSTQDYPSSLIGTIALIRQ
                     TFYDASWYGKAMNSKNRPDVNISLDALNAQFSAKLPLIFEVANTQDIFRVMQIGKEFG
                     QTYIIKSAGYEYERIEALKQNGVSLIVPVNFPSAYDVEDPDDANNITLAQMKKWEWAP
                     ANPGALAKAGIIFALTSAGLQDNKSFWPNIHKSVAMGLPADKALEALTTRPAKMLGID
                     AQVGTLEKGKLANLVIASGNLFEKNTTITQSWIKGRSYEVNRVPAIDPRGVWNLQGDL
                     KAYVLMINGTLSKLSGNFMYRDSQNVEVTPLINGDMLSIQFRGDSIGEPGLVRLKALL
                     INGRLSGTGEFPSGKTMSWSGTLFKSFEPKAEDNSIKSDSSVLSPISFPNMAFGLKEI
                     PVQQTVLIRNATVWTNTSKGILKETDVLFEKGKIKQVGKNLSAPAGAWIVDGTGKHVT
                     TGIIDEHSHIAVSRGVNEGTQSVSSEVRIGDALDAEDSDIYRQLAGGVTSSHLLHGSA
                     NAIGGQTQLIKLRWGAAAEELKFPNWPGFIKFALGENVKQSNWGDDFT"
BASE COUNT         5258 a         4277 c         4824 g         5493 t
ORIGIN      
        1 tttgagcggc ttggtaacgc cgtatttggc acgcaacgcc ataagtcccg gcatttcggc
       61 ttcggcgagt ttgatttctt tgcggcccca ctcggcaaga ctcatatcct taactttgta
      121 aggaacgtag gccggcacat tcaatttttc ggcaacagac attgtagact ccttatttta
      181 tcgctaacaa agtttcaatt ttttttgaaa ctataatcga aatgctttat aaaagcaaac
      241 gaattatttg tcttcgtccg gtaaattttc cgacaaagcg tccgcggcat cttcggccca
      301 gttttgaaat tttttccgat cttctttcgt ccagcgggcg tccccgtgag tgatcagata
      361 aatcggcatc ggcatctttt cgctacgcac ctcgccggcc atttcattta atttttcaac
      421 ctgacgatgg gcgttgtatt ttttccagtc ggaaaaattc aaatggcggc gcccttcatt
      481 gacatcgtgt gcgaccagcc atgaaatcgg cgctatttcg ctgtaccacg gccaaacggt
      541 ttcatgcgaa tggcaatcgt tgcaagcggt ctttatcatg gttttgatat cgtccggaat
      601 ggtgacatgt ttttcgagcg tacgttccgg atcaatggga ggattggtac gttcggggcg
      661 aataaactgt atggcgataa aaagaaccgg taaaccgata gccaaccatt ttaagacacg
      721 cttcatagat tatcctttct tctacatcta aaatacaacg gaggaaaata ttgttttggt
      781 ttgttatttt ccattgaatt tctacatttc gaagattcag ttatgattta taacgattac
      841 cggagaatat catgaaacga ttttttataa ctgctttttt gtttacttcg ctgttgtctt
      901 gcggtaagac gttgcaggaa gaagcggatg cttttttgaa atcttataat caagagtatc
      961 aggcggttta ttatgaagcc agcaaagccc aatggaaagc actcacggac atcaatgcct
     1021 taaacgacag tttgagtgtt ttagccaata aaaaatatgc tgcgtttgca ggcagcaaag
     1081 acatcattca gaaagtacgg tttttcagag aacaccgttc ggaactcaat gacataacga
     1141 tacgtcagct tgataaaata tggctggacg cgtcgcatca acccggtacc atgcctgatg
     1201 tagtggatca actgatcaaa gccaatacgg aagcgaccag tgcattgtat agctaccagt
     1261 ttacggttaa cggcaaacct tattcagcga atgatattga tcgtgcgttg cgcaattctt
     1321 ccgatctcaa ggaacgcgag gcggtttgga ccgcgtccaa agaagtcgga aaaatcgaac
     1381 gtgaccatct tgcccgactg caggagctgc ggaataaagt agcacgcgaa atgaagtaca
     1441 gttcattttt tgatctggaa gcgcacgatt atggaatgtc atcggacgaa gtgatccaac
     1501 tgatggatca attttcagaa gatctgaaac ctctgtatag agaactgcat acgtatatac
     1561 gctacgaatt agccaaaaag tataatcaac ccgttccgga gtatcttccg gcgcactggc
     1621 tgcccaatcg ttgggcacaa aattggccgg gattagttcc gggattgcag ggtgaagggg
     1681 atatacccgg aaaatccaaa gaatgggtcg ttgagcaagc tgagaaattt tacatgagca
     1741 tggggtttga aaagctgaac gataattttt ataaaaattc tgatttgtat cctgccgatg
     1801 ccgcaagcgg acgaaaaaag aatacccatg cgtcggcatg gcacctgaat ctggatcagg
     1861 attaccgttc gctgatgagt gtcgaaccca atatggaatg gtttaaaacg acgcatcatg
     1921 agctcgggca tatttattac tacatcgctt ataccaatcc tgaagtgccg cttaccttgc
     1981 gtgccggagc taatcgtgcg taccatgaag cgatgggtga cctgatggct gtggctgcat
     2041 cgcaagaggc ttatttagcc agtttaggtt tatttgataa aactaagacg cccgatgcga
     2101 tcaaactttt gctcaacagc gctttatcca atagttcgat cgtttttctg ccatttgctg
     2161 cgggtacgat gacacatttt gaatacgaac tgtacgagaa aaatcttccc aaagaagaat
     2221 ttaataagac atggtgggta ctggcaaaaa aatatcaagg tatagttcca ccgaaagagc
     2281 gtggtgccga atattgtgat gcggctacca aaacgcacat cattgacgat ccggcgcagt
     2341 attatgatta cgccatttcg tgtgttttga aattccaaat gcatgaccat attgccaagc
     2401 agatactcaa gcaagatccg gccaattgca attattacgg aagcaaagaa gccggtgagt
     2461 ttattcgctc gattatgcgt ccgggcgcga gcaaagattg gcgtcaagtt ttgaaagaaa
     2521 aaacaggtca ggacttgtct gcaaaagcca tgttggatta ttacggcccg ctttatgact
     2581 ggctgctcca acagaataag ggaagaaaga aagccgagtt ttgagaaaaa aataagagtt
     2641 atgaatgcaa agttatgagt ttataattta gaattttaga tttgcgatta gacgattgca
     2701 tgtgactcat ttgcataaaa cgtttgaaca acgtgagggt tgcgtagttg atgatgaaag
     2761 ggattgctga attgagcggc tatccggtgg tttattcaac gccggtggca tggggcgaaa
     2821 tggatgcgct ggggcatgtc aataacatca tgtactttcg ttattttgaa agtgcgcgct
     2881 ccgattattt tcaacgcatc ggtgtatggg atcatgcacg ggaacacggt gtcggtgtta
     2941 ttctacattc gacgcagtgc cgctttaaac gacctctgac ctatccggat caaattcacg
     3001 tgggcgctac gacggtcgag gtgatggccg atcgttttcg catgaaatat gccgtatgga
     3061 gtgaagcgca acaggccatc gtggccgaag gcgaaggatt gatcgtcgtg tatgattata
     3121 atcaaaatca gaaatgccca atgccgtcgt ggatgcgcga agcgattgct gaattagaaa
     3181 aaaaggcgag tcagccgaca aatttgtaac ctagacggtg gacggtgata aaatactgag
     3241 gccgtttggg attgggttcc aatttttgac gtaaccatgc catgtgcaca tccaccgtgc
     3301 gcgttgtgat gtcttcgcca taattccaaa cgttctcaag caattcatcc cgggacaaag
     3361 tcgcattgcg atgctcgatc atatagcgca gcaatcgaaa ttcctgagcg gagagctcca
     3421 ccgttttttt atggacgcgt acttcggctt ttcgaaaatc cacctcaata ttgttgaaac
     3481 tgtaattatc cgacgcctcc atgcgattgg ccgactcact gcgacgcaat agcgcttcga
     3541 tacgtgccgt gagctcgctc atatcaaaag gtttcgtcag ataatcatcg gcgccgagct
     3601 tgagtccgat cactttatcg gtcgtctggc ttttggcggt caacatcaat ataggcgtac
     3661 gatcgccgtc accgcgcaaa tcgcggcaca cgtccaagcc tgaactttgc ggcagcatca
     3721 catccagcaa aataatatca taacgattct ttcgggcttt gctcaaagcg atggtgccgt
     3781 tttcagcgat gtccacgacg aatccttcac gccgtaagcg atccgacaaa gttataatta
     3841 aaccgggttc gtcctcgacc agtaataatt ttttcacgtt ccctccggaa gaaaaacact
     3901 aaacgtactt cctttaccgg gttcgctctc aacacggatc gtgccgctat gcaatgcgac
     3961 ataacgttcc acgatgctga gcccgaggcc attgccgtga atctgtgcat cgcgtacacc
     4021 ggacgctctg aaaaacggtt caaaaatgtg aggtatatct tttttatcaa tgccaatacc
     4081 acagtcggtt atatcggtgc gaatatacgg cgattcatcg gtcaacgtga tacgtatcgg
     4141 tgcattggcg cgattgtact tgatcccatt ggcgatcaga ttgttaaata tcgtttgcaa
     4201 agcttcatga tttccgataa tcatgttatg tgtcgtttgg cggtcttcga aataaatcgg
     4261 ggtgtcattg gggaatgagg cgatgcattt ttggataatt atggataaat ccaaacgctc
     4321 catttttaat gagtttttat cggtgatgat acccgaaaaa tccaaaatct gatctaccat
     4381 gtccgatagc cgtttgcttt caccacgtat caactcgcca taacgccgca catcatcggc
     4441 atcggtgacg atcgcatcgg aaagattttc accggctgaa cgaataacgg caagcggcgt
     4501 tttaagttca tgtgaaactc cggcaacaaa ttccaattgc tgatttgcta atattctttc
     4561 ctttcgctgt gcgatcaaaa gaataacgag ccctgaggcc atgataagca gaacgcctgc
     4621 gctaagaaaa aggtttcgcc accgtacgat tttgacggca tcttccaatg agccttcgcg
     4681 atgtatgacc cgtaaattgc aacctgacgt cttggtgtcg gcatgcaact gcgttacgat
     4741 cgaatcaatg atcggattaa atgcgccgtt tgacgtacgt tcgcttgttt caatggattg
     4801 cgttacggtc aaggttttca tagttgtttt atcacgggtg cctttgacgc gaaccataaa
     4861 catatcttgg gtgcgaatac gggcgatctc agcgctgata tccgattttg accagtgtgc
     4921 gatcggcaaa ttagaattgg atttgtaaaa aaatttagat tggtcatcat tgatcacagc
     4981 gatatctaca tcattttcga acgatgcacc aaagtgaata cctgctaaac tatccatcca
     5041 tgatttgacc gcattcgtat ccaaaagaac agcaatgatt tcctcagccg gttgctccca
     5101 cggcaggttt agcattttgc gcatcggatt gacggtcacg atgatatatg ggtagtcggc
     5161 aagaaggtgg ccttgcatga taatcagatt gtttttttcc ttaccgaccg agcgcatagc
     5221 gcgcatccgt tgtaatttga gaacgatggg gtttgacact gtgtcggaaa cccgatgaag
     5281 ttcgggtctt gccgaatcca acaaaaaaac ctcaggcgtg ttattttcag acatacgacc
     5341 gtaaaaaacg gaacggatca aatcctggcg tgcggcattg gtttgccatt tattgacggt
     5401 agtttgaaga atattgggta actgttcagg ttgatcaaag tccacccgaa aaagatattc
     5461 gtgcaggcgc gatatttcgc tgtcaaaatc cgatgccata cgcgtagcgc tgatatggag
     5521 attactccgc atgcgcgtca attcgccttc gctcagtcgc cccagccaaa tatattgcat
     5581 tgtaccaata ctgatcagcg ctatggtaag cagcgcgata atccatccgg ggtgtaatcg
     5641 ttttgatttt gccatagtga aaaataggca ttcgaacagc ggtcttcaat cgaaatgtgt
     5701 gcggcgtttc atattttaca tagtttacaa ctcttttacg ccggcttaac aactctagtt
     5761 ctaaaatcaa gtatcattag cgtaattcat catcgtctat aaaacctgga aggaacgcag
     5821 tatgaaaaaa ataattctca tcttaggtat gaccttgttc ggtgtcggag gaaatgcatt
     5881 cgcgcaaggt attcgctata tggctacggg tcctgtgcag ccggtggatg aagtcggggt
     5941 tatatttgct gaaaaagaga acggtttgat tgccgatttg atttttcctg aacacgcatt
     6001 acccaaaggt tataaacctc aagggattga aaaaaatgat gtgttggtga tggttaacgg
     6061 ggtttcggta aaaaccgcaa acgagttgaa agcggcttac gataaagtca aggtcggtga
     6121 tacgatcaaa ctcgggctta aacgcgggga tgaaaaaatg attgccagtt ttgccaaaat
     6181 cgatcccgag catttaccaa aacgtaaaat gatcaaacgc accccggacg gaaaagaaat
     6241 agaggaaacc aaataatgtt aaggtggttt tattttacga ttctattctt ttgcttcacc
     6301 gattccggct tgatggcgca gtataatgca tttaaccagc gtgatgataa atatccgctg
     6361 ttgggattaa aacgcgccaa agaggtttat gaatcggaaa aagccgagta cgagcgcgtg
     6421 aaacaattgt ttgataaaca actggcttcg ccggcggaac tggaacgggc ccgacgttca
     6481 ctggccgacg tagaggtgaa ctatcagcaa tcgctgttgg cggtgctgtt tgaacagcaa
     6541 tttatcacgg tgtcacgcgc ggttaaatat caggacaaac gcggacagaa gcgtgttcgc
     6601 gtcgaactga tgaacacttc cggcggtgaa gctgaatttc gtaagttgat ttccatggaa
     6661 gatgagttat tccgctcctt acaaccggat cacataaata atatctacgt ttccgtaacg
     6721 aacacggaag gtgcgatcat cagccaaccg tacgaatcca aagtcaacga attggtctac
     6781 ggacagcccg tgacattgga ttttgtaatg ttacaagatt tggatgtggt gagcgtcaat
     6841 attatttacg gtaacggggc atcgcgaagc atgcggattt tcttacaaaa agacgctact
     6901 gaaaataaag tattattcca atccgagcag ttttcacagg aagcggatct cgggagtaat
     6961 gccacatttg atcttacgtt ggagttattc agcggcacgg acaatacgtt taagctggaa
     7021 gtattgaatc tcccgccgga actgacgcgc tattttcttg atgcggcaac caatgctaaa
     7081 ctcagtcagt taaagtttgc cgaatcggcg cgtacccgta aggcatcgct ccgcgttttt
     7141 ttgccggatc gtcctaccga acaagtccaa atcgaaaaaa ctattccgtt ttatgtatta
     7201 gcgataccgc aggaaaagca ggcactcatc caaacacaac gcgataaggt gtggtcccaa
     7261 caagagatcg aatcgctcaa cgcaggctat ctccgacttg aactcatcgt caaaggcaat
     7321 gcacgtttat tggtcaaagc gccgaagttg tattatgcca cgacagaatc cgagcctgta
     7381 gaagcatcgc ttgaaattcg caacgacggt tcacgtcgtc tggataacat caagtttgaa
     7441 gtggatctgc ctttggaatg gaaaaaggaa atacaaccgc tgatgattcc ctcgatccat
     7501 gtaaacgaag agtcggttgt gcgattcaag ttttatccgc cggaacacgt ggccgtcggt
     7561 aaatacgaag tgcgcatccg aacatcatcc gtctctgata atcagccggt caacgccgaa
     7621 gataaagtaa tcactattga tattcaggcg ggaagtaatg ctttgggcac gatggcgctg
     7681 atcgttctga ttctaagcat tatcggtgga atagtttggt ttggcattaa actcacacgt
     7741 aaatagaggt atttatgaca gtacacaatc aacccgtttt agaagcggca caattgacca
     7801 aacgttatga cgatggcgct ttagcgctta acggtatttc gtttcaggtt catcccggtg
     7861 aaatctatgc gatgcttgga gggaacggcg ccggtaaaac gacaacgatt aatatttttt
     7921 tcaatttcat caccccgaca tccggcgagg ccaaagtcaa aggaatcatc acgcatcagg
     7981 aacctttaag ggccaaagaa caaatggctt ttgtgtcgga gaatgtgatg ctctatccta
     8041 attttacagc gatggagaat cttgagtttt ttgcaaagct gggcggacgc gcagatataa
     8101 ccgaatcgga aatgaaagtc acgttatcgc gcgtcgggct acaaagcgaa gcgatgaaca
     8161 aaaaagtccg aactttttcc aaaggcatgc ggcaaaaatg cggtatagcc atcgctatta
     8221 tcaaaaaagc tccggccatt attttggatg aacctacatc gggtttggat cccaaggcag
     8281 gatatgaatt taaccgatta ctgatcgaac taaaacaaga gggtaaagcg atcctgatgt
     8341 ccactcatga tattttcaga gcaaaggaag tggctgatca tatcggaatt atgaaccgcg
     8401 gctcaatagt gatgcagaaa aaccgcgatg agttggctca tgaagatttg gagaaactct
     8461 acattgaata tatggcgggt tatatggaac cggtagccgt atagaactaa acattagtaa
     8521 ggtggttatg ataaaattat ttttcatggt atggttcatt agttcactta ctgtagctca
     8581 atcacgtgat gtacaagtaa gtggtttaga ttcattgata cactcggctg tacgagacgg
     8641 gtttcatgga ctggtatgga ttgaaagcaa cggaaaaccg atctactaca aaggttttgg
     8701 ttttgctaac gaaagtaata accaaaaatt tgacagtacg gtttttgtac aaatcggttc
     8761 tacggtcaaa gattttacgc gtgttgcgat ttatcaacta atcgaaaaaa aattgttatc
     8821 tctcgacgat ccgctgtcta aatatatatc cgaccttgca ggggaaaaga ataagattac
     8881 cgttcagcac ttattgaatc ataccgccgg gtttcctatc ggcatcaaat ttgatgcgga
     8941 gccgctgacg aaagaagaaa tggtcgaaca tatcagaggt ttaaatttac tctcaagtcc
     9001 gggagtgcac gaaaaatatt ctaatctagg ttattcatgc cttgcttatg tcattgaaaa
     9061 agtaagcggg cgttcatttg atgcctatgt ggatgagtac attctgaagc ctttaggcat
     9121 gcatcatacc ggcacatacc gcccgaattt tgatcgcaat aaggtagccc atggataccg
     9181 tcaaaatgcg gatatcggta ttattttgga tatgccgcat gatcaagacg ggcatctatg
     9241 gagtttacga gggaacggtg gttatttatc tacgtttagg gatatgaaac ttttttttga
     9301 ttcatttgaa acgggcaaac tattgagtac gccggcattt cgcatggccg tttatgactc
     9361 caaacaacct acggtgttgg ccggatcaga tatgatttcg ttctatgggt ttgccaattt
     9421 tccaggtatc aaagcgcggc tcatgattgc cagtaatcat gataactaca tggggcatca
     9481 attgttagaa tccattgagg ggtatttacg caacggtaaa aatcctatgg ggaaagttat
     9541 caaaaccgtt gaagtagata accccggcga gagtgcaaag gtttttgatt tacccaaaga
     9601 gggtgcggga ttgacgatcc ggcgatatgt tgaggccttc aatagcggca attcgaatac
     9661 gatgaaagcg ttttttgaaa attatgccaa aagcggagac ggttttgctc cgatggaacg
     9721 gcgtttgcaa aattataatc gtttgttttc ggatttaggt gttataaccg tgaattcatt
     9781 tcaaacacgt gcggatggtt cttgggaagt acatgtgggc tcggcgaaag ctaaagcaat
     9841 ttttaatttt caaatcgaaa caaaagcgcc ttggcgtttt gaagctttac agatcgaatt
     9901 aggcgattag tttcacaaat taaatataaa actatatgtt tacgttactg ttgaaaaaag
     9961 aaatacgcga tattttaaac tcacccaagt tttccgcgct tttcgggatg ggatcgttgc
    10021 tcatattgtt ggcgttttat atgggtgcgc gtaattatca agttctgcaa aaccaatacg
    10081 aaagcgcggt gcgagaaaat ttacgccaat tggaaggcgt gacagattgg tcgcgcgtcg
    10141 aaccacaaat acatctgcca ccttcgccgt tagccagtct tatatccggt gtgagcaacg
    10201 atatcggtcg ctcagtacaa gtgcaggccg gtgccgccga attggttcct gatcatacgc
    10261 gttatggtga agatcccgtt tacgcggcat tccggttact ggatttggaa tttgtatttc
    10321 agttaatttt atcgttagtg gcgatattgc tggcttacaa tgctgtcaac ggcgaacgcg
    10381 agcagggtac gctcaaactg atgatggcgc atgctgtgcc caaagataaa gtgatttttg
    10441 ctaaacttgt cggcagtttt accgcaacgg ctctgccttt gctggttcct atattgatcg
    10501 gctgcggtat attgatggtg atgaatgtac cgctgaccgg agcggagtgg ctgcgcctga
    10561 tgctgatcgt atgttcaggg atgttgtatt atttcgtttt tcttagtgtt tctatcttgg
    10621 tttcctcgct gacggcccga tcggcccatg catttcttgc gcttttgatt atctggattt
    10681 tcaccgtgct gatcgtaccg cgtacggccg tgctcttagc gggtcgggcg atcgaagtgc
    10741 cgtccgtgga tgaaatcaac tcacaaaagt ttcgtcatca atcgcagctc tttaatgaac
    10801 attttcaaaa aatcggcgaa tttaaaccaa attcaaacga tcctgaaaaa gcaatgaatg
    10861 aatttaacga atttatggac aaagcgcgcg ctgatcggga aaagaaggtc gtcgaattta
    10921 atgaacgatt aaatgaagac cgcgataacc ggcaaaaaat acaacagcaa tgggcttttg
    10981 cattgggacg tatttcaccg gcgacgtcgt tttcgctcgc cgccatgcaa ttggcgggga
    11041 catcttcgga acttcgtaat cgttttgaag agtccgtcaa atcgtataaa cagacattca
    11101 aacaatttgt ttttgaaaaa acgggaaaag cgcccggtgg taatatgatg atgattcgta
    11161 tcggcgattc gcctgaagaa aaaccgaaac ctatcaatcc ttacgactta ccgcaatttg
    11221 tgatgccatc gctgactttt tcagaaataa tcaatgccgc tttgccggat atggccatat
    11281 tgatcgtcat caatatgctc atatttgcgg ctactacggc tgcattcctt cgttacgacg
    11341 tacggtaaaa ggagaaaccc atgttatcgt tattgatcat caaagaattt aaaaacatac
    11401 tgcaaagtcc taagtttata gccacattta tatcgtgtac gctattgatc attctcagtg
    11461 tgtgggtcgg aatccgagaa tacaagctgg ccgtgaaaga ttacgaaacc ggcatagcgc
    11521 ttaaccggca ggatttgagt gaacgaacca attggatgaa tgtaggtaat cgggtatatc
    11581 gcaaaccgga tcctatgcag atatggacct tcggagttca ttacgatatc ggacgcgtcg
    11641 cccatgtcaa tacatgggat tcacccgaac tgcgcagcag catttattcc cgcgatctcg
    11701 tatatgccgt atttcggttt ttggatttta attttatagt tgtcgtggtg ttatcgttat
    11761 ttgctatcgt ttttacgtac gatgcggtaa gcggcgaaaa agaaagcgga atgttgaagc
    11821 ttacgttttc taatggtgtc cctaaatcca catacatcgt tgcaaaaatt atcggatcat
    11881 gggcggggtt ggggattcct ttgatcgtgc cgatgctgtt atcatttttg atgacgatac
    11941 tatttcaaat acctctcaca cccgatcact atgctcgttt aggcggttta attttggcgg
    12001 gtgcgctata ctttactttt tttattattt tgggaattct gatttcatca tggacttcgc
    12061 actcgaacgc atcgtttctc actttacttg tattatgggt tacgatcacc tttgtcgtgc
    12121 cgcgcctcgg tgttatgcta tcggggcagg ttttaccggt gcccacagcc gccgaagtgg
    12181 atagccaaat cgaaggcttt tctaagtcac aatttgaaac attgcgccaa aacctctcgg
    12241 aaacctggat gaaacgcaat aatgacgtgc aacaacagcc ggaatccgag cgacagacgt
    12301 accgcgataa gatggagtgg acatggctgg aagaagatca aaatgcccgc aagtccgtac
    12361 aggaaaacat tgaaagtttt acacgcaaag tacatgaaga tatggataac cgaaaacgcg
    12421 agcaaatcaa actgggtttg atttttgcgc gctggtcgcc gacttcgtta tttcaactcg
    12481 cggcaatgcg tttttcacag accgatattt ttctgaaaga acgttacgac gacgcgatac
    12541 gtacctatca aaaagatttt tccgatgttg tggaaaaaaa gcgtcgagaa aatagggccg
    12601 aaggactgcg tatcgaggtg gatagcgatc gcgggtttcg tgtgcaaaca ccggatttga
    12661 aaaaaacgat tgatgtgcga tccattccgg aatttacaca tccggccgtc tccgccgggc
    12721 atgcatggcg attatcggtt tgggatattg tactcctcgc cgtgataaca ggatttgttt
    12781 ttttgggagc gttggtgcga tttctgcgct acgatgtgcg ctaatacgtc aatacttagc
    12841 caatgacgga tctacggttt cggcgtacgc gatgataccg ccgcgaagat tgatgagatt
    12901 atcaaagccc agcgtgcgca gttgcgcgat ggccttggcg ctgcgtccgc ccattttgca
    12961 atgaaccacg accggtttgt tttgtgcgat gcgggccgat tcttgcataa tctgcgcaag
    13021 aggtattaat gtgccgccga gattgcaaat gccgtattca aatgactcgc gtacatcaat
    13081 gagttgaaaa tcttttccgg tatctatcca atgtttgagt tctgccggtg aaatttctcc
    13141 gccagtttgt ttttcgggtt cggaaattcc gcaaaaaact tcatagtcta tcaatgcggt
    13201 gatggtctgt tgcaggccat tgatcggatt ggccggatcg cgcttaaatt tcatagttcg
    13261 tgattcaaac gtcagcgcat tccacagaaa aagtttgccg ctaagcggcg ttccgatacc
    13321 ggtgatgact ttgatggctt cgcttgcttg catgctgccg ataatgcccg gcaaaactcc
    13381 gaggacaccg ccttcagcgc aactgggaac cataccgggc ggcggcgggg tggggaaaag
    13441 gtcgcgatag tttggaccga gtgtaccgga tggatctgta taattaaaaa ccgaaacctg
    13501 gccttcgaat tgaaatattg atgcgtagat cagcggttta ttgagtaaca cacaggcgtc
    13561 attgaccaaa taccgtgttt gaaagttatc gcttccgtcg gcgatgatgt cgtaagatcg
    13621 tatgatatcc aacgcgttag ctgaagtgag tttttcgcga tgggtattaa aatgtacaga
    13681 cgggttgagc tgcttcaaac ggtgcgcggc ggtatcggct ttgggtttac cgacatcatc
    13741 cacagtatat aggacttgcc tttggaggtt acttgcatca acggtgtcaa agtcaactat
    13801 gccgatagtt cctacgccgg cggctgtaag ataaagcaaa catggtgcgc caaggccccc
    13861 tgcaccgatg accaatacct tggctgattt gagtttttgt tgcccttcga ggccaaattc
    13921 tttgagaatc agatgacgat tgtatcgtgc taattcttca gtggtcaatc cgtacataca
    13981 atacgttgcc taactatgtt cgacgatcat cgcgccgcct tgaccgccgc cgatgcataa
    14041 cgtggctaaa ccgaactgaa gattttttcg cttaagctct ttgagtacgg tcaaaacaag
    14101 ccgcgttgca gatgaaccta cggggtgacc aagcgcaata gcgccgccat tgacattgag
    14161 gatttggcga tcaatttcac ctaccgcttc ggttaatccc aatttttcct cggcaaattt
    14221 tttagaggca aaacaacgtt cattggcgat cacctgcgcg gcgaaagctt cattcatctc
    14281 aattcgttgt atatccttca tggagaggcc ggcttttttg agcgcgacgg gtgtggcata
    14341 taccgggccg agtcccatgc gttccggatc aaggcctgca aagccgaaac cgcgaatgga
    14401 gccgagcggt gtatatccta acgctttggc tttttcttcc gacatgacga ggacaaacgc
    14461 cgcgccatcg gtgatttggc tggaattgcc ggctgttacg gtgccatatt ttttatcaaa
    14521 cgccgggcga agtttaccca gcgcttccat agtctgattt tttcgaatgc cgttatcttc
    14581 gtctgcgatt tttttaaaat caggtggtaa atacaccggc atgatttctt ctttaagaat
    14641 accgttgtcc gtggctgtcg tggcgcgttg atggctcagg agtgccagtt cgtcctgctc
    14701 tttgcgtgtg atgccgtatt ctttggctaa tacttcggcg gtttcgccca tattaagtcc
    14761 gcaaaacccg tcggtaagtc ccaagagaag tcctatcacg ggtttgaaat caccggggcg
    14821 aaacgaggtc atggcgccga tgcgtgccgg catagaacgg gcttgcatca tcgcggcaaa
    14881 tttattggtt gcacgttccg aaaaaagcaa aggcacctta gtcatggatt ctcccccgcc
    14941 ggcgatgatg acatcggctt caccggcgat aattcgcaga taggcatcgg caatagattg
    15001 cataccgctg gcacaattgc gcccgacggt aaacgaaggt acgtgtttgg ggactcccgc
    15061 ctccagagcg atgatgcggg cgacattggt ggcttcaggc ggattgccga tgttgccgac
    15121 gatgacttcg tccacgagtt tgctgtcaag attggtgcgc gccatcagtt cacgcatcgc
    15181 cagtttcccg agatcaaccg ccgacatttt attgaaaagt gttccggctt ttatgtatgg
    15241 cgttcgcacg ccgtcaacga taactacctt tttcatatac gactcatttg ttggaatacg
    15301 atttttttat cagttcgtcc aaatattttc tgggatcatc aaaaacttcg ggcgttacaa
    15361 gaaaatgggt tttaccggcc agttttaacc cgtattcgta atctttaaag agctctgccg
    15421 cgatcgcccg gaaagagcgt ttttcggttt gtgatcgttg agcgattttt tgaatggcca
    15481 catcggaaaa ttcaaaaata atgccgtgcg tcgtgagata ctgctgttga aattctttca
    15541 tcgaagaata ggtgagcagc ttgttgagtt cggcttgcgg ctgcaaaacg acgtctttgg
    15601 tgacgcgaaa tttacgaata ttcgtggacg gtaatttctt ttcaaacttg atcaatacgt
    15661 tttctaaaat attgactaag ccgcgggcac ctgttttttg aataaacgca cgttctgcaa
    15721 aaatgtgcaa ggcatcgtca tcaaattcga cttcgatgcc gtaagcggca aaatcgcgct
    15781 ttttgctgag gatcacgctg ctgttgggat tttttagaat attaaatagt gcgtcaaccg
    15841 taagttcgtt gagtacggcg atgacgggca ggcggcctac aaattccgat tcgaatccgt
    15901 attcgacgag gtcttctgtt tttacgcgtt tcatcagttc accgcgcgag agtttagaga
    15961 tgccttcttg atccgagccg aagccgatgc gtacttgatt gatgcgcttg cggatgtttt
    16021 cttcgatgcc gccgaaagcg ccgctcatga cgaataggat gttttttgtg tttacttttt
    16081 tacgttgcac tttgccggtg cgttgggctt ccatcgcggc ttccatttgg gcggccatgt
    16141 cgtgcgggac acgcagatcc acttcggatt cttccatgag tttcaaaaga ttccgctgaa
    16201 cgccggtgcg ggatacatca ataccgcggt tgttttgtgc cgaagcgatt ttgtctattt
    16261 cgtcaagata gataataccg tactcggcgc ttttgatatc gccgtcggct tcacgtacaa
    16321 gatcgcgcac gagatcttcg acatcgcccc cgacatagcc ggtttcggaa aacttggtgg
    16381 catcgccttt gacgaaaggt acaccgattt tttttgcgat cagtttgacg atgtacgttt
    16441 tacccacacc cgtagggccg atgagaagaa cattgctttt gatgttacct acaagttttt
    16501 gttcatccgt gagcgtgtct tccagtttca tacggttgta gtgcgtggcg attttggtgg
    16561 cgatgacttc gatcgcttct tcctggcatt cgacgaattg gttgaggtat tcttccaatt
    16621 cctcaggctt caggtcaaaa ttggtttttt caccggattt cagcgttgaa ccctgatttt
    16681 tcgtgcctgc actgtcttgt tccgggtcgg ccgtaccgta tttttctttt aaaaattctt
    16741 caaactcttt tttgaactgt tccgaattgg tctgcgacga aatattcatc gttggtgttt
    16801 tcctttggtt gattcttagc ataacgaaac aagtcgtgac ttatttcatc aatgctggat
    16861 caacttactg ggattcctga tgaaattcaa gcgattagct gtgttgtgtg ggctctaccg
    16921 cccgtcgcat ttttcgtgct aaagcaccgt atgaactata ccaaagttgt tgagctattc
    16981 tgcacgatca atccggcaaa tgtagcatcc ggcttccgca ttgcgcgcat ggaggtatgc
    17041 gacatctgta tgtttttcaa aaatgtcgct gatcatttga tctacatcgc gatctccgac
    17101 aagacgggtg tataccatac gctgatcatc ggaataacca atgagggtta atggaaagtg
    17161 ctttttatcg gctttgattt ccggaggaaa tcggtgtata tcttcatagg gcgttacgga
    17221 ttcggcatga ataaatacag ggccggactg gttgaaggcg ttatctactt caaacggaga
    17281 atgcgaaaag agcaaacgtc tgtccgttcc gggcttgaag ggtttcagcg aaacgcggca
    17341 cgggccgtag ccgctcgcaa tttgttcgac gacggtgtga ccttgatcgt ccattcggtc
    17401 ggtgcgaatg cgttctgcta agtttaaagg caggggaacg attttgaatt tagtcataaa
    17461 aatctcctta aaatttgttt tatgctgaat gataaatagt tcatgacgtc caaacgaccc
    17521 gattcttgct cttgttgtag gcttttgttg aaatttgctt tcattactta gaaacctttg
    17581 tatatttgtc gccgtatatc gtctgtccca atatttcatt tcgtctgaat gtatttcttt
    17641 taccataacc cagtgaggtg tgatgaaacg atttttatgt tttgcaatgt gcggtttgtt
    17701 gtgctttacg ttgaaaaatg tttctgcgca gcaaacatgg ccggtcgagg gtctgcatga
    17761 taaaacgcct tcgattcatg catttgtcgg tgccacgatt attcccgggc ccggtcaaaa
    17821 aattgataac ggcacgatcg tcatccgcga cggtgtcatc gaagcgatcg gcaaatccgt
    17881 taatataccg cgagaggccg tcatccatga tctcaaggga atgtggattt atccggggtt
    17941 gattgacgcg tacgccggtt acgggatcgc accgccggca aaagagaagg ggagcgacgg
    18001 tccgcaacct tatacatcca aaaaaggcgc ctacagttgg aatcaagccg ttaaaccgga
    18061 atttgaagcg gcgcgggaat ttacaataga tgccaaacgt gcgggcgaat tacgcgcgtt
    18121 gggatttacg accgttcacg ccagaccgtt ggacggtatt gtgcgcggca ccggagcgat
    18181 tgtcaatttg ggcgagtctt cggcctccga agaaatttta agtcgtcagg cgacggccaa
    18241 tttttcattc gataaaggcg tttctacaca agattatccg agttcactga tcggtacgat
    18301 cgcactgatc cggcaaacat tttacgatgc atcgtggtat ggcaaagcga tgaattccaa
    18361 aaatcgtccg gatgtgaata tttctctcga tgcgctcaat gcgcagtttt cagctaaact
    18421 gcctttgatt tttgaagttg caaatacgca ggacattttc cgcgtgatgc agatcggtaa
    18481 agagtttgga caaacgtaca tcatcaaatc cgcgggatat gaatacgaac gcatcgaagc
    18541 gctcaaacaa aacggcgtat cattgatcgt gccggtcaat tttccttcag cgtatgatgt
    18601 cgaagatccg gacgatgcga ataatatcac tttagcacag atgaaaaaat gggaatgggc
    18661 tccggccaat cccggcgcat tggccaaggc gggtatcatc ttcgcattga cttcggctgg
    18721 tttgcaggac aacaaatcgt tttggcccaa cattcataaa tctgtcgcga tgggattacc
    18781 ggcggataaa gcgctggaag ccctgacaac gcgcccggcc aagatgctcg gtattgacgc
    18841 acaggtcggc acgctggaaa aaggaaaatt ggctaatctg gtaatcgctt ccggcaattt
    18901 atttgaaaaa aatacgacga tcacccaatc ctggatcaaa gggcgttcgt atgaagtcaa
    18961 tcgcgtgccg gcgatagatc cgcgtggcgt atggaatctc caaggggatc tcaaggctta
    19021 cgtgctgatg atcaatggta cgctctcaaa gttgagcggt aattttatgt accgcgattc
    19081 gcaaaacgta gaggtaactc ctttgattaa cggggatatg ctgtctatcc agtttcgcgg
    19141 agattcgatc ggcgaacccg gacttgttcg gttgaaggcg ctgcttatca atggtcgcct
    19201 gtccggcacc ggagaatttc cttcaggtaa gacaatgagc tggtccggaa cattgttcaa
    19261 atcgtttgaa ccgaaggccg aagataattc gatcaagtcg gattcgtccg tattatcacc
    19321 gatcagtttt cccaatatgg ctttcggttt gaaagaaatt cccgtacaac aaacggtatt
    19381 aatccgaaac gctacggtgt ggactaacac gtcgaaaggg attttaaaag aaaccgatgt
    19441 gttgtttgaa aaaggtaaaa tcaaacaagt tggaaaaaat ctttcggcgc cagcgggtgc
    19501 atggatcgtt gatgggacgg gcaaacatgt gacgacgggt attattgatg aacactcgca
    19561 catcgcggtg agccgtggcg tcaatgaagg cacgcaatca gtgagttcgg aagtgcgcat
    19621 cggcgatgcc ttggatgccg aggattcgga tatatatcgc caattggccg gtggggtcac
    19681 atcgtcgcac cttttgcacg gctctgctaa tgcgatcggc ggacagacac aattgatcaa
    19741 attacgttgg ggtgccgcgg ctgaagaatt aaaatttcca aactggcccg gctttatcaa
    19801 gtttgctctc ggtgaaaatg tcaaacagtc caattggggt gatgatttta cg
//
LOCUS       DAOQNQ010000027        19750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_952_length_19750_cov_6.666260, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000027 DAOQNQ010000000
VERSION     DAOQNQ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19750
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_952_length_19750_cov_6.666260"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2478
                     /gene="priA"
                     /locus_tag="PK754_03290"
     CDS             <1..2478
                     /gene="priA"
                     /locus_tag="PK754_03290"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNB08559.1"
                     /translation="FTYGIPDAWESVIVPGSRVLAPFGRKKITGYVVALSRHSERDQI
                     SPIHEALDPLPAFSEDLLKLAEWISDYYMAPLGQVLGAMLPPGMERHGEKLYVLKAVI
                     TDFEIKSLSKTKPVQSKILKALYVYKKLTLRQLVKKVGARNLIKNLDELLRSAVVEER
                     DVISDQLANIRTDTWVRLSSLLLGDEERFVWATDELQKRAPKQADILFYLDEATRQSE
                     DEEPFALRQAELLKITEAPYSALIALEKKNWIERIEREVIRDPYQRDYPRPNDVTLND
                     HQAQAVAAIAETLRNNKYYTWLLYGVTGSGKTQVYIESIRQTLALGKNAIVLVPEIAL
                     TPQAVERFKAHFGDQVAVLHSRMTMGERFDSWRKLQSGHFRIAIGARSAVFAPVQNLG
                     LIVVDEEHEHTYKQQDNIPPYHARDAAIMRASFCKATVILGSATPSIESYYNATTQKY
                     GLLKLPIRINDVPMPEVEVVNMREEYEKHTSDWQPILSRALRQRMKEELQHRRQAILF
                     QNRRGYSSSVECFDCGHVAECPRCSIALTYHITGHKLRCHYCGFHQPMYSECPKCDSL
                     SIAQQGIGTQKVEEQLQMAFPNAGVLRMDVDSTSAKGAHHRILEEFKNKNALILLGTQ
                     MVTKGLDFENVTVVGVISADTSLLLPDFRAGERTFQLLTQVAGRAGRKNKIGHVIIQT
                     MHPDRAAIRHARQHDFTSFFNEEISIRHDLLYPPFGRLMLIQIRSEDEQKAVELSSVF
                     SDILAGEIKRYPDLASGVAQLGPTPAPIVKIRNYFRYHILLKVDKEADRSGSRIRSIL
                     RHTRKQVISRIPSGAEIRIDADPLSLL"
     gene            2491..3813
                     /locus_tag="PK754_03295"
     CDS             2491..3813
                     /locus_tag="PK754_03295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB08560.1"
                     /translation="MSKRFEEKREAFEILNKELSDLIPLEQRVQKKDKSPHVVDPHAH
                     LPNDPAKSDATGAVPKEWLDEQAALHEKYKLVERVYKFYRNIIQNMSSSIICMDMAGH
                     ITFLNANAAKTLEYQVEELLGHPMTDIVAEPGRESKIIDRMLIGNKRFESKEVGLIAK
                     SGAVLPIGFSTAPLIENHEQIGVIITFRDLTEMNLMRKQVERMDRLATLGELATGIAH
                     EVRNPLGGIKTAAQVLEESFEDNDRRLELVSRIVREIDRVNHLLTDFFKFAKPVKPTR
                     DHFDVESLIDGVYLLLAAQMNSKKIRFKEDFSSTLPKVYADPHQTEQVLMNIILNAVQ
                     AMPQGGDIIVRTYTTTVGESKSMIHHTDEFFDKNIPFVAIEISDTGVGIPQENIEKIF
                     NPFYTTKPDGMGLGLSICNRLLTENNGNLHVESAVGKGTKFVIMLPTR"
     gene            complement(3794..4165)
                     /locus_tag="PK754_03300"
     CDS             complement(3794..4165)
                     /locus_tag="PK754_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015115875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HNB08561.1"
                     /translation="MNPLTLKQHALWLRAVEEFNRKQFYICHETLEALWRDTNEETLK
                     RLLQGVLHIAVALYHLDRNNAVGYELQMKKGLVKLNAGLRVPSDLPIRNDLIVFYREV
                     ADFNRMRKSHIPMLRFNASAA"
     gene            complement(4162..4608)
                     /locus_tag="PK754_03305"
     CDS             complement(4162..4608)
                     /locus_tag="PK754_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNB08562.1"
                     /translation="MKTVSAIISGREPYSVTLGTNVFDVIQLMASKNIGAICIVDSSA
                     TMRLRGVFSERDLLKRVVLQGLDPRALTIETVMTKNVAVASAGESYDVCMERMKRVNS
                     RHLPVVEGEKFLGMISVRDLLETNLEEKDAELKMMNAYIHDMPATV"
     gene            complement(4713..5153)
                     /locus_tag="PK754_03310"
     CDS             complement(4713..5153)
                     /locus_tag="PK754_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08563.1"
                     /translation="MSEISPPNTKLPILRIIWGAVIFSLFVFVAVVHLVILPTLGEKE
                     NESLSSLPFPVHYVFMFIAAGAAVAFVFLRQKRDITVAELSHDPKAAEQERIKRSILL
                     FAVAEMIGLLGCSILAFGAPYAQGMGLWGISFILLAMEFPRTED"
     gene            complement(5567..6499)
                     /locus_tag="PK754_03315"
     CDS             complement(5567..6499)
                     /locus_tag="PK754_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08564.1"
                     /translation="MGRKISPSDLLANSFLRMAKQFNANVELIKVLKSRTYKIGDANV
                     LVRASSDGNKRYFFGINYITVEEIANLENPFIAFICGSIERIVIIPAKLLFNHLHQIS
                     HDRNGEYKINIDQDLNIVLSGRSNRLECKTFINNWDLLLSPPKFDENEKPKTVEESLH
                     SVLQGRLLEIGNIRGYQTFCPDKSRKFNDKNLEEVSTLKTCPELQFSDYDLLRQIDVI
                     WFKTRGNNYIPEYAFEVELSTGVWSGVGRMATLMDYSNVGLYVIANDSKKYSQVINSF
                     TEYQDRYKFIANDLVGELYSAELNLKQLRIDIGL"
     gene            complement(6492..6692)
                     /locus_tag="PK754_03320"
     CDS             complement(6492..6692)
                     /locus_tag="PK754_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08565.1"
                     /translation="MEADNEVLFRITKEDLREEALHYLGREMNEEEYTKAKKLLEFGA
                     GENLRYIYYGVFLEIMDKSNNG"
     gene            complement(6725..7258)
                     /locus_tag="PK754_03325"
                     /pseudo
     CDS             complement(6725..7258)
                     /locus_tag="PK754_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009422404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
     gene            complement(7279..7758)
                     /gene="smpB"
                     /locus_tag="PK754_03330"
     CDS             complement(7279..7758)
                     /gene="smpB"
                     /locus_tag="PK754_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNB08566.1"
                     /translation="MATATEKKKYEYKSIATNRKARFEFEIIDTLEAGIALTGSEVKV
                     LREGKINIQDCHARIENGEVILYHMHIPEYKQANYNNHEEYRPRKLLLHRVQIRKLER
                     QVQEKGMTLVPLSVYFNERNRIKVQLAVARGKKLYDKREAIAKKDQKRTEQREYKVR"
     gene            complement(7762..8703)
                     /locus_tag="PK754_03335"
     CDS             complement(7762..8703)
                     /locus_tag="PK754_03335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB08567.1"
                     /translation="MTDPNYSISVVIPLFNEEESLPELTEKIHTVASRMGVVYEIIFV
                     DDGSTDNSYTVIQKLKQQYAAVKALRFRKNFGKSTALSEGFKMATGTYVITMDADLQD
                     DPEEIPHLVNKLQEGNDLVSGWKKVRHDPISKTIPSRFFNYVTRVMSGIRLHDFNCGL
                     KAYRREVVKDVNLYGELHRYIPVLTKWEGYRRITEIVVHHHPRKYGQSKFGLSRFLKG
                     FLDLITVVFLTRYVKRPLHFFGFFGSLSLLAGLGICGYLSFDWFLGTPIGQRPILFLG
                     ILLIMIGFQIISTGLIGEMLVHNFNRQKEVPVKERIE"
     gene            complement(8716..9537)
                     /locus_tag="PK754_03340"
     CDS             complement(8716..9537)
                     /locus_tag="PK754_03340"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HNB08568.1"
                     /translation="MHYHTKYHRSFFLSFFGFMALILFSGCGAPKEVIRQPADITTVT
                     PLEVLRRCNENAAKIRHIKSVATMNVESPKSGGQFTAQIAIRLPDSVYIKIEGMLGID
                     GVKASLNRESFVVYNIINKQVIIGKTSASAIRRTFDYNVNYDELVELLVGLPRVPESE
                     LVRLREFSVDDTYFVMQFATGEGLRKIWFDPYAGFAVSRIVDYDSTGDVSVEREFSRF
                     QKIDGRYFPKYVRLVRPREQDLLSLFFDMRTVNESFNSSIFKIKYPRDIDIVEMQ"
     gene            complement(9521..10840)
                     /locus_tag="PK754_03345"
     CDS             complement(9521..10840)
                     /locus_tag="PK754_03345"
                     /inference="COORDINATES: protein motif:HMM:NF020855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB08569.1"
                     /translation="MHSQIGTQDSRYDAYKPAAVQHLVDALIADMSGEFGEAEMHYRF
                     ALLSDSNALPVSNALAEDYLELGRFDEALALVQRVLIKDPFNPDALEVLADIHIRQHN
                     FDKAIQTLELLSRRYPSNIDAHYRLITIYEVQGKAKEAAVHYARLLDVIGANTLLSLK
                     LGEIYMRSRAYDKAVDVYHRARSSDPNNTEILSALAMAYRFNKEIPKAIEIYESLAEL
                     QEDNLQVHASVGLLSIQSGNYEKAVRAFRHADRLQPNNFDFLRSLGFALVQLKRSVEA
                     LDVLERASMLNGRDLLTMNLLAPIYQNQKRFTQSDSVFERILVFEPNNEVILNNYSYS
                     LAERRIRLDKARQMIEKALKKAPDNGHYLDTMGWVLFQLEEYAQALIYTQRAVAVHPN
                     SEEVWTHLGEIHHKLGNTQDALSAFQKALTINPDNSMLTEKINALSH"
     gene            complement(10957..11292)
                     /locus_tag="PK754_03350"
     CDS             complement(10957..11292)
                     /locus_tag="PK754_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08570.1"
                     /translation="MKKIQLKKIAHARSGDKGDSSNVGLIAYKQEYYSILCAQATPEA
                     VRRHFSKIANGPVDRYEVPNLLALNFIIHDSLGGGGTESLKTDAQGKTHGMGLLEMEI
                     EVPDDLIVA"
     gene            complement(11289..11546)
                     /gene="xseB"
                     /locus_tag="PK754_03355"
     CDS             complement(11289..11546)
                     /gene="xseB"
                     /locus_tag="PK754_03355"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNB08571.1"
                     /translation="MSKKKQNEESSETTFEASLKELETIVDALEHGDVPLDETLVKFE
                     RGMKLVEFCQAKLNTAEQKLKILIKDKDGNYTLEEEDEEAV"
     gene            11767..13605
                     /locus_tag="PK754_03360"
     CDS             11767..13605
                     /locus_tag="PK754_03360"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB08572.1"
                     /translation="MVLRIALWLCVAWPAMAQIKVVVFPFHNTTQAQKNDWLSHGFAE
                     ALNTELAKDTALDVIDRTDVLAEIKSKGWKQADIKDEAKAMAVARSLEANRMVYGVFD
                     AKDSSIKVLTKFFDLRTGVADGKNTYIGEGKFTTNNVHAMYGQIANKALVSFGRKTID
                     EIGGPTKGAINVDAYEPYIKGILKYDESSTVDDYNKAIEMLTQAATIDTGFALAYAGI
                     AKAYAKIARIQDVSGKLTEKEESYNKALEAGLRAVRIDKRLAVGWTALALTYRELKDR
                     DKLIEAARKAVQIRVSQYDAYDMLADAFSPSFFPQYKNIDSSIFYRKKSVTYNTKFAS
                     GFRGLGGDYMDKGDYKNAEQAFQKAVSINPKHAGSRDRLGQIYFAQGDYIKAKDNFSE
                     AIRLDAKTPFGYTHLADVLYMEGKYAEAIAAYDSALHHNPKFAMAHYGLAWTLLSSKQ
                     RSLRDSVRALTHATQAVEISGQKNAAFLSVLAEAQFANGNKEKAIETITLARALDSAN
                     TDYELSETRYAGKDKSGDYAYAMRRGQMFWKQGRGADGVKEFEEANKLNPKNVHVLLG
                     LARYYDQDKQYKKAFDHYFLARACDWDKKYVKQIQERMNALEKHSK"
     gene            complement(13663..14037)
                     /locus_tag="PK754_03365"
     CDS             complement(13663..14037)
                     /locus_tag="PK754_03365"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNB08573.1"
                     /translation="MSHATDTGKHGENLAEIFLKQKGYDIRHRHFRFRNGELDIVAES
                     KTFIVIVEVKTTDLRRHDESAFGEPETWLTSKKQKFLKQAAEYYLAKYPAPEKDCRFD
                     LITVRLRSDGEEIRHIENAFWM"
     gene            complement(14194..15633)
                     /gene="dacB"
                     /locus_tag="PK754_03370"
     CDS             complement(14194..15633)
                     /gene="dacB"
                     /locus_tag="PK754_03370"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNB08574.1"
                     /translation="MMWRYGCILFFTAASLFSQYRSTQLDSVRRAIDAVYTDDFFKDI
                     RTGFFAKSLLTGQVIHQKNPDSLRSTASCMKLVTTAAALDRWGTNKSFRTEFRYDGAI
                     KNGVLNGDLYIRGLGDPYFLPEILLRAIYHLKAMGLTEIRGDIVGDDTYLIDTENAET
                     NDRAYSAVGGALGFNFNIINICVRPGEKTGDSAIVFIEPISSLVKVINGVKTTDSGEG
                     IGVNHNNTFIRYNGNQMTVALYGTIAKNENEFVIYKRVPNPTWWTIANIKETLGIMNI
                     KVSGGLRTGKTPKASKILIDPPSYDLSYIVSGINKWSNNYVVGQLLMMMGAEAFGEPG
                     TDEKGIRALQPFLRKVGISEKEIQMADGSGLDERNKMTTRAQVRLLEYMYRDFRYASD
                     YIASLSIGGIDGTEKKRFIKRKDESSLIGTTRLKVGFLYGVSGLSGYIETQSKDIIAF
                     SILTNGYPKEYYETMRQFEDRIVKALGGL"
     gene            complement(15640..16116)
                     /locus_tag="PK754_03375"
     CDS             complement(15640..16116)
                     /locus_tag="PK754_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HNB08575.1"
                     /translation="MTFKKGPIQGVILKNHKKFVDDRGWLTELYRQDELASENVPVMG
                     YTSLTLPGVARGPHEHVQQTDLFCFIGPSNFKLYMWDNRKDSPTYGHKMVVFVGEDNL
                     QSVIIPPGVVHAYKNVGGKPGIVHNFANTLYAGKGKKEPVDEIRHEHDPQSPFQLD"
     gene            complement(16123..16479)
                     /locus_tag="PK754_03380"
     CDS             complement(16123..16479)
                     /locus_tag="PK754_03380"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNB08576.1"
                     /translation="MKNETKETVVESTDPAEIKKKRNRRILFWSLIGIGALTIISGNY
                     GAYQIYKIKQQRAAVEKEIEKLKREHAVLADQRDRLKTDLEFIEKIAREKYTMIREGE
                     RVYQVLSKSETTNKKK"
     gene            complement(16491..17771)
                     /gene="eno"
                     /locus_tag="PK754_03385"
     CDS             complement(16491..17771)
                     /gene="eno"
                     /locus_tag="PK754_03385"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009053203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNB08577.1"
                     /translation="MSHIHHVHAREVLDSRGNPTVEVEIHLESGAMGRAIVPSGASTG
                     EHEAVELRDGDKKRYLGKGVQKAVHNVNTIIAKKIMHMEAGDQFTLDHTMIALDGTAN
                     KGKLGANAILGVSLAAARAMANEVNIPLFQYIGGIQANTLPVPMMNIINGGAHADNNV
                     DFQEFMVMPVGARSFTEALRMGAEVFHHLKTVLKGKKYNTAVGDEGGFAPNLKSNDEA
                     IEVILQAIQAAGYKAGKDIMIALDPASSEFYQNGKYVFKKSDGRKLSSEQMAAYWENW
                     VRQFPIISIEDGMAENDWEGWKHLTDAVGKKIQLVGDDLFVTNPKFLAKGIEKGVANS
                     ILVKVNQIGSLTETIDAVQMAHKAGYTAVMSHRSGESEDTTIADIAVALNCGQIKTGS
                     ASRTDRIAKYNQLLRIDEALGDRAHFPGRKAFKV"
     gene            complement(17814..18788)
                     /locus_tag="PK754_03390"
     CDS             complement(17814..18788)
                     /locus_tag="PK754_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNB08578.1"
                     /translation="MTLDDVFDPIEDELEIFKTKFSTQLTSKIKIVDTIARYVVSSRG
                     KNLRPVLVMLCSKMCGGSVTERSYRAAITIELVHTATLVHDDVVDGADMRRGLPAINA
                     IWKNKMAVLMGDYLLGNALISIVDIKSFDAMMLLSVATKRASEGELMQIDKSRKLDID
                     EPTYLRMISDKTGALIAAACELGAITTTEKPEDRKALNEYGELIGVAFQIKDDLFDFE
                     GEQTIIGKTKGRDLKEQKITLPLIYAFSRAPKSESKAVLKKIKKSVTSGDIKQIVAFT
                     EHYGGIEYAKKRLQEYSDRAIQTLNYFPDSPSRQALKKFVQYNIQRNK"
     gene            19297..>19750
                     /locus_tag="PK754_03395"
     CDS             19297..>19750
                     /locus_tag="PK754_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB08579.1"
                     /translation="MPEQPSTQRRFEELWAGRGYSTRIQNFQELMRFKVRDILLISSL
                     YDSYILEEDGRLFELIQEEYRGLDMNQAPDIIHVSNGTEAIRMLDRQHRFDLILMTLH
                     IEDIHAIALAKKIRSLGHEMPIIVLAYDNIETQEVMTHRDADIFENIFI"
BASE COUNT         5327 a         4676 c         4287 g         5460 t
ORIGIN      
        1 tttacgtatg gcattcccga cgcatgggaa tccgtcattg ttcccggttc gcgtgtactg
       61 gcgccgtttg gccgcaaaaa aatcaccggc tatgtcgtag cattatcccg ccacagcgaa
      121 cgcgatcaga tatctccgat acacgaagca ttggaccctc ttccggcttt ctccgaagat
      181 cttttgaagt tagcggaatg gatatccgac tattatatgg cgccgctcgg ccaggtgctc
      241 ggcgctatgc tcccgccggg catggagcgt cacggtgaaa aactgtatgt gctcaaagcg
      301 gtgattaccg attttgaaat caaaagctta tctaaaacca aacccgtaca atccaaaatt
      361 ctcaaagcgc tttacgttta taaaaaactc acgttgcgcc aattggtcaa aaaagtcggt
      421 gcgcgcaatt tgataaaaaa tttggacgaa ctgttgcgct cggctgtcgt cgaagagcgg
      481 gatgtcatca gcgatcaatt ggctaatata cgaacggata cctgggttcg tctctcgtcg
      541 cttctgcttg gcgacgaaga gcgttttgta tgggctacag acgaactcca aaaacgcgct
      601 cccaaacaag ccgatatttt attttacctg gatgaagcga cgcgccagtc ggaagatgaa
      661 gagccttttg ccctacgcca ggccgaactt ctgaaaatca ccgaggcgcc gtattcggcg
      721 ctcatcgcat tggagaaaaa aaactggatc gaacggatcg aacgcgaagt gatccgcgac
      781 ccgtatcaac gcgactaccc cagacccaac gacgtcacac tcaacgatca tcaggcgcaa
      841 gcggtcgctg cgatcgccga aacattgcgt aataacaaat actacacatg gctgctctac
      901 ggtgtgacgg gcagcggtaa aacgcaggtt tatatcgaga gcattagaca aacgctggcg
      961 ctcgggaaaa acgcgatcgt actggtaccc gaaatcgcac tgacaccgca agccgtagaa
     1021 cgtttcaaag cgcatttcgg cgatcaggtc gcggtactcc acagccgtat gacgatgggc
     1081 gaacgatttg acagttggcg aaaactgcaa agcggacatt ttcgtatcgc catcggcgca
     1141 cgttcggccg tatttgcacc ggttcagaat ctcggattga tcgtggttga tgaagagcac
     1201 gaacacacct acaaacagca ggataatatc ccgccgtatc atgcccgcga tgcggcgatc
     1261 atgcgggctt cgttttgcaa agcgaccgtg atcctcggtt ccgccacgcc cagcatcgaa
     1321 tcctactata acgccacgac acagaaatac ggcttactca agttgcccat tcgtatcaac
     1381 gacgtcccaa tgccggaagt ggaagtcgtc aacatgcggg aagaatacga aaaacacacg
     1441 tcggattggc agccgattct ctcccgtgca ttacgccaac gcatgaagga agaattgcag
     1501 catcgtcgtc aggcgattct ttttcaaaac cggcgcggct attcgagttc cgtcgaatgt
     1561 tttgactgcg gtcatgtcgc cgaatgccca cggtgcagta tcgcgttgac gtatcacatc
     1621 accgggcaca aactgcgctg ccactattgc ggttttcatc aaccaatgta ttcggagtgt
     1681 ccgaagtgcg atagtcttag catcgcccag caaggcatcg gcacgcaaaa agttgaggaa
     1741 caattgcaaa tggcatttcc caatgccggt gtgttgcgga tggacgtgga cagtacgtcc
     1801 gccaaaggtg cgcatcatcg cattttggag gaattcaaaa ataagaatgc gctgatttta
     1861 ttgggcacac agatggtcac caaagggttg gactttgaaa acgtcaccgt cgtcggtgtt
     1921 atttcggcgg atacgagttt gttgctgccg gattttcgcg ccggcgaacg tacttttcaa
     1981 cttctcaccc aagtcgcggg ccgcgcaggg cgaaaaaata aaatcggaca tgtcatcatt
     2041 caaaccatgc accccgaccg cgcggcgatc cgtcacgccc ggcagcatga tttcacttcg
     2101 tttttcaatg aggaaatctc catccgtcat gatttgttat atccgccgtt cggccgatta
     2161 atgttgattc agatccgttc ggaggacgaa caaaaagctg ttgaactatc gagtgtcttt
     2221 agcgatattc ttgcaggcga aatcaaacgc tatcccgatc tggcttccgg cgtggctcaa
     2281 ttaggcccga caccggcgcc catcgtgaaa attcgaaatt attttcgtta tcacatttta
     2341 ttgaaagtgg acaaagaagc ggatcggtcc ggttcccgta tccgttctat tctccggcat
     2401 acgcgcaaac aagttatttc ccgaattcca tccggagccg aaattcgcat tgatgcggat
     2461 ccgctttcgt tactttaatc cgataaagtt atgagcaaac gatttgaaga aaaacgcgaa
     2521 gcctttgaga tactcaacaa ggaactcagt gatctcatac cgcttgagca acgcgttcaa
     2581 aaaaaggata aatcgcctca tgtggtagat ccgcacgcgc acttgcccaa cgatcccgcc
     2641 aaaagcgatg cgaccggcgc cgtacccaaa gagtggctgg acgaacaagc cgcgttgcac
     2701 gaaaaatata aattagtcga gcgcgtttat aagttttacc gcaatatcat acagaatatg
     2761 agttcgagca ttatctgtat ggatatggcc ggtcacatca ccttcctcaa tgccaatgcc
     2821 gccaaaacac tggaatatca ggtcgaagaa ttgctcggtc atccgatgac ggacatcgtc
     2881 gcggaacccg gccgtgaatc caaaatcatt gatcgtatgc tcatcggtaa caaacgtttt
     2941 gaaagtaaag aggtcggtct gattgccaag tccggcgccg ttttaccgat cggattcagc
     3001 accgcgccgc tcatcgaaaa tcacgaacaa atcggcgtga ttattacgtt tcgtgatctg
     3061 acagaaatga atctgatgcg caaacaagtc gaacgaatgg atcgtctggc gactttgggt
     3121 gagttggcca ccggtattgc gcatgaggtg cgcaacccgc tcggcggcat caaaacagcg
     3181 gctcaggtgc tcgaagagtc cttcgaagac aacgaccgcc gattagaatt ggtaagtcgt
     3241 atcgtgcgtg agattgatcg cgtcaaccac ttactgacgg attttttcaa atttgccaaa
     3301 cccgtcaaac cgacccgcga tcactttgat gtcgaatcgt tgatcgacgg cgtttatctt
     3361 ttacttgcgg cgcaaatgaa ttctaaaaag attcgattta aggaagattt ttcctcgaca
     3421 ttacccaaag tgtacgccga tccgcatcag actgaacaag tattgatgaa catcattttg
     3481 aatgccgtcc aggccatgcc gcaaggggga gatatcatcg tacgtacgta cacgacgacc
     3541 gtcggtgaaa gtaaaagcat gattcatcat acggatgaat tttttgataa aaacattccg
     3601 tttgtggcga tcgagatcag cgatacggga gtcgggattc cgcaggaaaa tatcgagaaa
     3661 atattcaatc ccttttatac caccaaaccc gacggcatgg gcctcgggtt gtctatttgc
     3721 aatcgtttac tcaccgaaaa taacggaaat ctgcacgtgg aaagcgccgt cggcaaaggc
     3781 accaagtttg tcatcatgct gccgacgcgt taaaacgcag catcggtatg tgcgattttc
     3841 gcatcctatt aaaatccgct acctctctgt aaaacacgat cagatcattg cgtatcggga
     3901 ggtcactcgg cacacgcaat cccgcattca atttgaccaa cccttttttc atctgcaatt
     3961 cataaccgac cgcattattc ctgtccagat gatacagcgc tacggcgata tggagcacgc
     4021 cttgcaataa tcgcttaagc gtttcctcat tcgtatcgcg ccaaagtgcc tcgagcgttt
     4081 catggcagat gtagaattgt tttcggttaa actcttctac cgcacggagc cataacgcat
     4141 gctgcttgag ggttagcggg ttcaaacggt ggccggcata tcgtgaatat acgcattcat
     4201 cattttgagt tcggcgtctt tttcttccag attggtttca agcaaatcgc gcacggagat
     4261 cataccgaga aatttttcgc cctccacgac cggcaaatgc cgcgagttaa cgcgtttcat
     4321 acgttccatg catacatcgt acgattcacc ggcggaagcg acggcgacgt ttttcgtcat
     4381 caccgtctcg atggtcagcg cgcgcggatc cagcccttgc aacacaacgc gtttgagcaa
     4441 atcacgttcg gaaaacacac cccgaagccg catcgtggcg gagctgtcaa cgatacaaat
     4501 cgcgccgatg tttttggaag ccatcagttg gatcacatca aacacattgg tacctaatgt
     4561 cacagaatac ggttcgcgcc cggagataat agcacttacg gttttcacgg caacctcctt
     4621 ggttggttgc aaaagacggt tgtttaagaa taatgagaaa gaaaggcggg taaaatataa
     4681 acgcaaaagc tgatgatgtc aataagcgcg catcaatctt ccgtacgggg aaactccata
     4741 gccagaagga taaacgatat gccccaaagc cccatacctt gagcatacgg cgcgccaaaa
     4801 gcgagaatac tgcaaccgag taacccgatc atttccgcaa cggcaaaaag tagaatcgaa
     4861 cgtttgatcc gttcctgttc ggccgctttc ggatcatgcg aaagttccgc gacggttatg
     4921 tctctttttt gccgaagaaa aacaaaagcc acggcggcgc cggccgcgat gaacataaaa
     4981 acataatgta cgggaaaagg caatgaggac aaactctcat tttccttttc gcccaaagtc
     5041 ggaagaatca ccaggtgcac taccgccaca aaaacaaaca acgaaaatat cactgcaccc
     5101 caaatgatac gaagaatagg gagtttggtg ttgggtgggc tgatttcact catggttatc
     5161 tccgttacaa cgttttgttg ttttgatgcc gaattatata cccctccgct tgtagcatgg
     5221 tagtgcattg cttttgatgt tgttttatgt tttcaaactt tcccgagatt gatctttcca
     5281 gcaatccgag tatattaaaa aaataaatag cctgtcatag tcgacaagtt gtaacatcag
     5341 ctttcgttag tcttacggct tttattttac gggtttgtct atgcgcgctg attgtatttt
     5401 tttaatcttg tttagcaaaa aaagttttaa ctgcataccc atccatggtt ttttaattac
     5461 cctgtgggct tttcataata cttattgatt gaggttcagt acttgcaaaa gcggataata
     5521 taaaacttta gcaaacgtct aaaaaatact tgttcttaag cttgtgttaa agtccgatat
     5581 caattctcaa ttgctttaaa tttaattcgg cagagtacaa ctcgccaact aaatcgtttg
     5641 ctataaattt atatctatct tgatattccg taaatgagtt aatgacctga gagtatttct
     5701 ttgagtcatt tgcaattaca tataatccaa catttgaata atccattaat gttgccattc
     5761 tgccaacacc gctccaaacc cccgtcgaca actcaacttc aaaagcatat tcaggaatat
     5821 aattattacc tctagtttta aaccaaatga catcaatttg tcttaataaa tcatagtctg
     5881 aaaattgtaa ctcgggacaa gtcttaagcg ttgaaacttc ttcaagattc ttgtcgttaa
     5941 attttctgga tttatctggg caaaatgtct ggtaccccct tatatttccg atttctaata
     6001 agcggccttg taataccgaa tgcaaacttt cctcaacagt tttaggcttc tcattttcat
     6061 caaactttgg tggtgataga agtagatccc aattattgat gaaagtctta cattcaagcc
     6121 tattagacct ccccgataat actatattca ggtcttggtc aatgttgatt ttatattctc
     6181 catttctatc gtgactaatt tggtgaaggt gattgaaaag taattttgca ggaataataa
     6241 ctattctttc tattgagcca caaataaaag caatgaatgg gttttctaga ttagctattt
     6301 cttcaactgt tatatagtta attccaaaaa aatatcgctt attaccatct gaagatgccc
     6361 ttactaaaac attcgcgtct ccaatcttgt acgtccttga tttcaaaact ttaattaatt
     6421 caacatttgc attaaactgc tttgccattc ttaaaaatga attcgccaat aagtctgatg
     6481 gagaaatctt tctacccatt attgctttta tccattattt ctaagaaaac accgtaatat
     6541 atgtatctta aattttcacc tgcaccaaac tcaagaagtt ttttcgcttt tgtatattct
     6601 tcttcgttca tttccctacc taaataatga agcgcttctt cacgcaaatc ttcttttgta
     6661 attctaaata gaacttcgtt atctgcttcc ataaaaattt cagtgatttt gtgattttga
     6721 atcaaacact aaccaaatcg tacttcgtaa gcttactatt cgatataagc gaattaaaca
     6781 atgaaacgtc tataccgaca gcatgcatgc cttgctccga gctttgaaca agtgttgtcc
     6841 cactaccgca aaacggatca agaatcacat cgccttcctt gaagtaagtt cgctttttga
     6901 aattatccgt atgtttgtca ataaagtatt ctacaagttg cggtataaat tttcctttat
     6961 agggatgcag cctgtgaacg tgtttagtcg tttctgattc tttgtattga tcaaatgata
     7021 atgcccaatt tagatcggaa cccaaatgat ttttccatga attttcgcga ttccgtttaa
     7081 attcactata gtagcttata agttcttccc gagaaataag cgtggatcca ttttcaccga
     7141 ttttttttat acgcccatat tgaatcaaat aagcgatatt agatgtagat acatcctttc
     7201 ctgtatactc tgtagcccaa tgactagctt cttttattgt aaacaattta tctttcatac
     7261 atgcccgttt atttgctact accttacctt atattcccgt tgctcggtgc gtttctgatc
     7321 tttttttgct atagcttcgc gtttatcgta cagttttttt ccgcgtgcta cggcaagctg
     7381 gactttgata cggttacgct cgttgaaata aaccgacagc ggcaccagcg tcatgccttt
     7441 ttcctggact tgccgttcga gtttacgaat ctgcacccga tgcaaaagca gtttacgcgg
     7501 acggtattct tcatgattat tgtagttagc ttgtttgtat tcggggatgt gcatgtgata
     7561 taatatgact tcaccgtttt cgatgcgcgc atggcagtcc tgaatattga ttttaccttc
     7621 ccgtaaaact ttgacctcac tgccggtcaa agcaataccc gcttctaagg tatcaataat
     7681 ttcaaattca aatcgcgctt tgcgattggt cgcaatagat ttgtattcgt atttcttttt
     7741 ttccgttgcc gtcgccatag attattcgat ccgttctttg acgggcactt ccttctgacg
     7801 gttaaaatta tgaacgagca tttcgccgat cagaccggtg gatatgatct gaaatccgat
     7861 cataatcaga agaatgccca aaaacaaaat cggacgttgg cctatgggcg tacccaaaaa
     7921 ccaatcgaac gacaaataac cgcaaatacc gagcccggcc aaaagtgaca gcgatccaaa
     7981 aaaaccaaaa aaatgcaaag gacgtttgac gtaacgcgtc aaaaatacga ccgtgatcag
     8041 atcgagaaat cctttgagaa aacgtgataa tccgaactta ctttggccat atttgcgcgg
     8101 atgatgatgc acgacgattt ccgtgatgcg acggtaacct tcccattttg tcaaaaccgg
     8161 tatgtagcgg tgcaattcgc cgtacaaatt gacatctttg accacttcgc gccgataagc
     8221 cttgagacca cagttgaaat catgtagacg aatcccgctc atcacacggg tcacatagtt
     8281 aaaaaaacgc gacggaatgg ttttggaaat cggatcgtga cgcacctttt tccaaccgga
     8341 aaccaaatcg tttccctcct gaagtttgtt aaccagatga ggtatttctt ccggatcatc
     8401 ctgcagatcg gcatccatcg tgatcacata cgtacccgtc gccattttga acccctcgga
     8461 caaagccgta gattttccga agttttttct aaatcgtaat gctttgacgg cggcgtactg
     8521 ttgtttgagt ttttgaatca cggtatacga attatccgtg ctgccgtcat cgacaaaaat
     8581 gatttcatac accacaccca tgcgggacgc gacggtatga attttttccg tgagttcggg
     8641 cagagattct tcttcattaa atagcggtat gacaacgcta atggaataat tgggatcggt
     8701 cacgcgggta tcctgttatt gcatctccac gatatctata tcgcggggat atttgatttt
     8761 aaatatactg ctgttaaaag attcattgac cgtacgcata tcaaaaaaca aactcaaaag
     8821 gtcttgttca cgcggccgca cgagacgaac gtacttagga aaatatcggc cgtcaatttt
     8881 ctgaaaccgc gaaaattcgc gctcgacgga tacatcgccg gtcgagtcgt aatccacgat
     8941 gcggctgacg gcaaacccgg catacggatc aaaccatatt ttacgaagac cttcgccggt
     9001 agcaaactgc attacaaaat aagtgtcgtc caccgaaaat tcgcgcagac gcaccagttc
     9061 ggattccggc acgcgcggca acccgaccaa aagctcaaca agttcgtcat aattgacatt
     9121 gtaatcaaac gtgcgccgaa tagccgacgc gctggttttg ccgataatga cttgtttgtt
     9181 gataatatta tacaccacaa acgattcgcg attgagcgac gccttgacac cgtcaatacc
     9241 gagcatgcct tcgattttga tatacaccga atccggtaaa cgaatagcaa tctgcgccgt
     9301 aaattgtccg ccgctcttgg gcgattcgac attcatcgta gccaccgatt tgatatgacg
     9361 aatcttagcg gcattttcat tgcaccggcg aagaacttcc aacggagtga cggtcgtaat
     9421 atccgcaggt tggcggatta cttctttggg tgcgccgcat ccgctaaata ggattaaagc
     9481 cataaaacca aagaaagata aaaaaaacga acgatgatat ttagtgtgat agtgcattta
     9541 ttttttccgt gagcatagaa ttatcaggat tgatcgtaag cgctttttga aaagccgata
     9601 acgcatcttg ggtattgccc aatttatgat gaatttcgcc cagatgcgtc catacttctt
     9661 cactgttggg atgaactgca acagcgcgct gcgtatagat aagcgcttgg gcatattctt
     9721 ctagttgaaa caaaacccaa cccatcgtgt ccaaataatg accgttgtcg ggcgcttttt
     9781 tgagcgcttt ttcgatcatc tgccgcgctt tgtccaaccg gattctgcgt tcagccaaac
     9841 tataactgta attattaagt atcacttcgt tgttcggttc aaaaacaaga atgcgttcga
     9901 atacgctatc cgattgggtg aaccgttttt gattctgata aatcggcgcc agaagattca
     9961 tcgtcagtaa atcgcggcca ttgagcatgg atgcgcgctc cagcacgtcc aacgcttcga
    10021 cggagcgttt gagctgcacc aaggcaaaac ctaacgagcg aagaaaatca aaattattgg
    10081 gctgtagcct gtccgcatgg cgaaatgcgc gtacggcttt ttcataatta ccgctctgaa
    10141 tagacagcag tccgacactg gcatgcacct ggagattgtc ttcttgaagt tcggcaagcg
    10201 attcgtaaat ttcgatcgct ttaggaattt ctttattaaa acggtacgcc atggccagcg
    10261 ccgatagtat ttccgtatta ttgggatcgc tactgcgtgc tctgtgatat acatccacgg
    10321 ccttatcata cgcacgggag cgcatgtaga tttctcccag tttcaatgaa agcagcgtat
    10381 tcgcgccgat aacgtccagc aaccgtgcgt aatgtaccgc cgcttctttg gctttgcctt
    10441 gcacttcgta tatcgtgatg agccggtaat gcgcatcaat attcgacgga tacctccggc
    10501 tcagcaattc taatgtttga atggctttat caaaattatg ctgtcggata tgaatatccg
    10561 ccaaaacttc caatgcatcc gggttaaaag gatctttgat cagcacgcgc tgaacgagcg
    10621 ccagcgcttc atcgaaacga ccgagttcta gataatcttc cgccaacgcg ttggacacgg
    10681 gcaatgcatt gctgtcgctg agaagagcaa aacggtagtg catttcggct tcgccaaatt
    10741 cgccggacat atcggcaatc aatgcatcca ccaaatgctg aacagccgcc ggtttgtatg
    10801 catcgtaacg cgaatcctgt gtaccgatct gcgaatgcat acgcgaacca caactccaaa
    10861 ggagcggcaa aatcaaaaga aagacggatg ccttataaat acggtttcgc ataaaaacag
    10921 atcgtgttat aaacaaatac attggtcgga attttttcag gctacaatca gatcgtccgg
    10981 cacttcgatt tccatttcaa gtaaacccat accgtgggtt ttgccctgag catccgtttt
    11041 gaggctttcc gtacctccgc cgccgagcga atcatgaatg atgaaattga gcgctaaaag
    11101 attgggcact tcataacgat ccaccggacc attggcgatt ttagaaaaat gccgacgtac
    11161 ggcctcaggt gtcgcttgtg cacacaagat agagtaatat tcttgtttgt aagcgataag
    11221 tccgacattg gaactgtcac ctttatcgcc gctgcgcgca tgcgcaattt ttttgagctg
    11281 tatttttttc aaacggcttc ctcgtcctct tcttcgagcg tatagttacc atctttgtct
    11341 ttgatcagaa ttttgagttt ttgttccgcc gtattgagct tagcctggca aaactctacc
    11401 aatttcatcc cgcgctcaaa cttaaccaac gtttcatcca gaggcacatc accgtgctcc
    11461 aatgcgtcca cgatcgtttc aagttctttg agcgaggcct caaacgtggt ctcggatgat
    11521 tcttcgtttt gtttcttttt actcataggt ataaccttac aaatcggcat tataataatc
    11581 attcccatca tcgtttgcaa cgatgttaaa tgaataacgg gacacaatgg ctcctcataa
    11641 gcctccgatt tttaaattaa aaaagtaatt cattttacta attttttatt gttgtgctga
    11701 acggtcttat gtatcttggc gctggtaacc gtttttttta atggaatcga aaccgcagga
    11761 gaacgtatgg tacttcggat agctctatgg ttgtgtgtgg catggccggc gatggcgcaa
    11821 attaaggtcg tggtattccc gtttcacaat accacacagg cacaaaaaaa tgattggctt
    11881 tcgcacggtt ttgccgaagc gctcaatacg gaattagcca aagacacggc gctggatgtg
    11941 attgaccgaa ccgatgtatt ggcagaaatc aaatccaaag gatggaaaca agccgacatc
    12001 aaagatgaag ctaaagctat ggctgtggcc cgttcattgg aagccaatcg catggtgtat
    12061 ggcgtatttg acgccaaaga ttcttcgatc aaagtattga ccaagttttt tgatcttcgc
    12121 accggcgtgg ccgacggaaa aaacacatat atcggcgaag gcaaatttac gacgaataat
    12181 gtgcatgcca tgtacggcca gattgccaat aaagcccttg tgagtttcgg ccgtaaaacc
    12241 atcgatgaaa tcggcggccc gacaaaaggc gccattaatg tggacgccta cgaaccctat
    12301 atcaaaggta ttctcaagta cgatgaaagc agtacggtag atgattacaa caaagccatc
    12361 gaaatgctga cgcaagccgc gaccattgat acgggatttg cactcgctta tgcgggcatt
    12421 gccaaagcct acgctaaaat cgcacgcatt caggacgtca gcggtaaatt gaccgaaaag
    12481 gaagaaagtt ataataaagc cctcgaagcc ggattgcgcg ctgtgcgcat tgataaacgc
    12541 cttgccgtag gttggacagc gctggcgctc acgtaccgcg aactgaaaga tcgcgataaa
    12601 ctcatagaag cggcacgtaa agccgttcaa atccgcgttt cgcaatacga tgcctacgat
    12661 atgctcgcgg atgcgttttc gccgagcttt ttccctcaat acaaaaatat cgattcgtcc
    12721 attttttatc gcaaaaaaag cgtgacgtac aatacgaaat ttgcctccgg attccgcgga
    12781 ttaggcggcg actacatgga taaaggcgat tataaaaatg ccgagcaggc gttccaaaaa
    12841 gccgtcagta tcaaccccaa acacgcgggt tcacgcgatc gcttaggtca aatctatttc
    12901 gcgcagggcg attacatcaa ggccaaagat aatttttccg aagcgattcg cttagacgcc
    12961 aaaacgcctt tcggatatac gcatcttgcc gatgtgctct atatggaagg aaaatatgcc
    13021 gaagcgatag ccgcttatga ttcggctctg catcataatc ccaagtttgc gatggcgcat
    13081 tacggtttgg catggacgtt actcagttcc aaacaacgat cgttaaggga ttccgtccgg
    13141 gcgctgacgc acgcgactca agctgtggaa atcagcggac aaaaaaatgc cgccttccta
    13201 tctgtgctgg ccgaagctca atttgctaac ggcaacaaag agaaagccat cgaaacgatt
    13261 actttggctc gtgcgttgga ttcggccaat acggactatg aattgtccga aacacgttat
    13321 gctggaaaag ataaatccgg cgattatgcc tacgcgatgc gtcgcggcca gatgttttgg
    13381 aaacaagggc gtggagcgga cggcgtaaaa gagtttgaag aagctaataa attaaatcct
    13441 aaaaatgttc acgtgctgtt gggattggct cgttattatg atcaggacaa acaatacaaa
    13501 aaagcgtttg accactattt tctcgcacgc gcctgcgatt gggataaaaa atacgtcaaa
    13561 caaattcagg aacgaatgaa cgcgttggaa aagcattcca aataaaactt cagttcacgt
    13621 ctgtaaaaaa acctcaaaca aaattgtttg aggtttttta tttcacatcc aaaacgcatt
    13681 ttcgatatgc cggatttctt cgccgtcgct tcggaggcgc accgtgatca gatcaaatcg
    13741 acaatctttt tcaggtgcgg gatatttggc caagtaatat tcggcggctt gttttaaaaa
    13801 tttttgcttt ttggacgtaa gccatgtctc cggctcaccg aaagctgatt cgtcatgccg
    13861 ccgaagatcg gtggttttga cttccacgat gacgataaaa gtttttgatt ccgcgacaat
    13921 atccagctca ccattgcgaa accgaaaatg ccgatgcctt atatcgtatc ccttctgttt
    13981 taaaaaaatt tcagccagat tttcgccgtg ttttccggtg tctgtagcat gactcatgat
    14041 gggatgccga aaattcttct gtgaattgcc cgtacgaaca taaaaacaga aaggtctgtg
    14101 ccgattaccg tttcggataa actcgcttta cgtggattcg tacgataaaa ataaagaact
    14161 atgtgttaaa ataaattttt tcgcagtaaa actttacaac ccccccagcg ctttcacgat
    14221 acgatcttcg aactggcgca tggtttcata atattccttc gggtaaccgt tggtcaaaat
    14281 actaaacgca atgatgtctt tggattgcgt ttcgatatac ccgctgagtc cgctgacgcc
    14341 atagagaaaa cccactttga gacgtgtcgt gccgatcaac gaagactcat ctttgcgttt
    14401 gataaatcgt tttttttctg tgccatcaat accgccgata ctcagcgacg cgatgtaatc
    14461 cgaagcgtag cgaaaatcac gatacatata ttccagtaat cgcacctgag cccgcgtcgt
    14521 cattttgttg cgttcatcca ggccgcttcc gtcggccatc tgaatctctt tttcggaaat
    14581 tcctactttc cgtaaaaaag gctgtaaagc acgaattcct ttttcgtctg ttccgggttc
    14641 accaaatgct tcggcgccca tcatcatcag gagttgtccc accacataat tattactcca
    14701 cttattgatg ccggagacaa tgtaagacaa atcatacgac ggaggatcta ttaaaatttt
    14761 ggacgctttc ggtgttttac ccgtacgcaa tcctccactg accttgatat tcataatccc
    14821 caaggtctct ttaatattgg cgatcgtcca ccacgtcgga ttgggtaccc gcttgtatat
    14881 gacaaattcg ttctcatttt tggcaatggt cccgtacaag gcaaccgtca tctgatttcc
    14941 attgtatcgt ataaaggtat tattgtgatt gacaccgata ccttcgccgg aatcggtcgt
    15001 cttaacgccg ttgataactt tgacaagaga ggagatcggc tcgataaaaa caatcgccga
    15061 gtcgccggtt ttttctcccg gacgaacaca gatattgatg atattaaaat tgaaacccaa
    15121 agcgccgcca acggccgaat aggcgcgatc gttcgtttcg gcattttctg tatcaatcaa
    15181 atacgtatcg tcacccacga tgtcgccgcg aatttccgtc aaccccatcg ctttgaggtg
    15241 atagatcgca cgcaaaagaa tttccggtaa aaaatacgga tcgccgagac ctctgatata
    15301 gagatcaccg tttagaacgc cattcttaat agccccgtcg taacgaaatt ccgtgcgaaa
    15361 acttttattg gttccccaac gatccagagc cgccgctgtg gtgacgagtt tcatgcacga
    15421 agccgtggaa cgtaacgaat cgggattttt ttgatgaatg acttgaccgg tcagaagtga
    15481 cttggcaaaa aaaccggtgc ggatgtcttt aaaaaaatca tccgtatata cggcatcaat
    15541 agcgcgccgt acactatcta attgcgtgga gcgatactgt gaaaataacg aagccgccgt
    15601 aaaaaaaaga atacatccgt agcgccacat catgaactat caatccagtt gaaatggcga
    15661 ctgaggatca tgttcgtggc gaatttcgtc cacgggttct ttttttcctt tgccggcgta
    15721 cagtgtattg gcaaaattat gaacgatccc cggtttgccg ccgacatttt tgtaagcatg
    15781 gacaaccccg ggaggaatga tcacactttg cagattatct tcaccgacaa aaacaaccat
    15841 cttatgaccg tacgtcggcg aatctttgcg gttatcccac atatagagtt taaaattgga
    15901 aggccctata aaacaaaaca gatcggtttg ttggacgtgt tcatgcggac cgcgcgccac
    15961 acccggcaat gtcagcgacg tataacccat gacgggtaca ttttcagagg ccaattcgtc
    16021 ttgacggtaa agctcagtca gccatccgcg atcatcaaca aactttttat ggtttttaag
    16081 aatcacaccc tgtatggggc cttttttgaa agtcatgaaa cgttacttct ttttgttggt
    16141 tgtttcggat ttggataaca cctgatatac acgttcgcct tcgcggatca tagtatattt
    16201 ttcacgggcg attttttcga taaattcgag atccgttttc aaacgatccc gctgatcggc
    16261 aagcacggca tgttcgcgtt tgagcttttc gatttctttt tcgacggcag cacgctgctg
    16321 tttgatttta taaatctggt atgcgccgta attgccggaa ataattgtca gtgcaccgat
    16381 accgatcaaa gaccaaaaaa gaatgcgacg attacgtttc tttttgattt ctgccggatc
    16441 ggtcgattcg accacggttt ctttcgtttc gtttttcata tttcgtattc ctacactttg
    16501 aatgctttac gtcccgggaa atgagcacgg tcgccgagtg cttcgtcaat gcgcagaagt
    16561 tgattgtact tggcaatacg atccgtgcgg gaggccgatc cggttttgat ttgaccgcaa
    16621 ttaagcgcaa ccgctatatc cgcaatggtc gtatcttcac tttcgccgga gcggtgactc
    16681 atcacggccg tataaccggc tttgtgcgcc atctgtacgg catctatcgt ttccgtcaac
    16741 gaaccgatct gattgacttt gacgaggatg gaattggcaa cgcctttttc aatacctttg
    16801 gccaaaaatt taggattggt cacaaaaaga tcatcaccga cgagttggat ttttttaccg
    16861 acagcatcgg taaggtgttt ccaaccttcc cagtcatttt ccgccatacc gtcttcgatc
    16921 gagatgatgg gaaactggcg aacccaattt tcccaatacg ccgccatttg ctcggatgac
    16981 aacttacgtc cgtccgattt tttgaaaaca tattttccgt tttgataaaa ttcgctggaa
    17041 gccggatcaa gagcgatcat aatatcttta ccggctttat atcccgccgc ttgtatcgcc
    17101 tgcaagatca cttcgatcgc ttcgtcattg gatttgagat tgggagcaaa tccgccttca
    17161 tcaccgacgg ccgtattgta tttttttcct ttgagtacag tcttgaggtg atgaaaaact
    17221 tccgcgccca tacgcaatgc ttccgtaaat gaacgcgcac cgacgggcat caccataaat
    17281 tcttgaaagt ccacattgtt atccgcgtgg gcgccgccgt tgatgatatt catcatcggc
    17341 acgggcaacg tattggcttg aataccgccg atgtattgga acagggggat attgacttca
    17401 ttggccatag cacgcgcagc cgcgaggctg acgccgagaa tggcattggc gccaagtttg
    17461 cctttgttag ctgtgccgtc aagtgcgatc atcgtatgat ccaatgtaaa ttgatcaccg
    17521 gcttccatgt gcatgatttt tttggcaatg atcgtgttga cattgtgtac ggctttttga
    17581 acaccttttc caagataacg ttttttatcg ccgtcgcgca attccaccgc ttcatgttct
    17641 ccggtagatg caccgctggg aacaatggcg cgacccatag cgccgctttc caagtgaatt
    17701 tcgacttcga ccgtaggatt gccgcgtgaa tccaatactt cgcgggcatg aacgtgatga
    17761 atgtgactca taactattgc tcccgatgtt gaaacgccgg tggcgtgaaa ttattattta
    17821 ttgcgttgta tgttatactg aacaaatttt ttgagcgctt gacgcgacgg ggaatccgga
    17881 aaatagttga gcgtttgtat cgcgcgatcg gaatattcct gtaaccgttt tttggcgtat
    17941 tcgatgccgc cgtagtgttc ggtaaaggca acgatctgct tgatatcgcc ggacgtcacg
    18001 ctttttttga tttttttcaa aaccgctttg ctttcacttt tcggcgcacg gctaaaagcg
    18061 taaatcaaag gtaatgtgat tttttgttct ttaagatcac ggcctttggt tttgccgatg
    18121 atggtttgtt ctccttcaaa atcaaacaaa tcgtctttga tctgaaaggc gacaccgatc
    18181 aattcgccat attcgttcaa cgctttgcga tcttccggct tttccgttgt cgtgatggcg
    18241 ccgagttcgc aggccgcggc gatgagagcg cccgttttat cggatatcat ccgcaagtat
    18301 gtcggttcgt caatatccaa cttcctgctc ttgtctatct gcataagttc gccttcgctg
    18361 gcccttttgg tagccacaga caaaagcatc atcgcgtcaa aacttttgat atccacgata
    18421 ctgatgagcg cattacccaa aaggtaatcc cccatcaaga cggccatttt atttttccaa
    18481 atagcattga tcgccggcaa accgcgccgc atatccgcgc cgtccaccac atcgtcatgt
    18541 accagcgtag cggtatgcac cagttcgatg gtgatcgccg cacggtagct gcgttccgtt
    18601 acggaaccac cgcacatttt gctgcacagc atgaccaaaa ccggacgcag gttttttccc
    18661 cgggaggaaa cgacataacg cgcgatcgtg tctacgatct ttattttgga agtcagctgt
    18721 gtgctgaatt tggttttaaa aatttcaagc tcatcttcga tcggatcgaa gacatcgtct
    18781 aaagtcatgc cgaagtccgt aaaactaaat ctttataaac ttgggacaat gtaggcgatt
    18841 gcaatatgca aatcaagatg cgaactgata tccgatgagt tgttattaaa cggcatatta
    18901 atgacctcct tccgtttctc tttgcaactg agcgaaagag aatacgatct cgctaaaacc
    18961 tcataattga atgcgaagtg ctttcgcttg aagcgttggc gttaacttaa gaattgaccg
    19021 gtataaggtc atcaatacaa tcaaacgttt ttgttcctct actctttgcg actctgcgag
    19081 agagcatatg aactcgttat gggctaagta cgcagaaaaa tatcaatgtt tttgtttcca
    19141 ttcggggaaa cgatattaca cataaccgat cttggacacg atctcataac ctttttttat
    19201 tttctttgtc ttccatccgc ttttttttac atttggtgcg gttgtaattc attttgttta
    19261 cataaggttt tcacaccgta ttttacggtt ttatttatgc cggaacagcc ttcaacacag
    19321 cgccgattcg aagaactttg ggcaggtcgc ggttactcga cgcgcatcca aaattttcag
    19381 gaactcatgc gctttaaagt gcgtgatatt ctgttgattt ccagcttgta cgattcctac
    19441 atactcgaag aagacggtcg tttatttgaa ctcatccagg aggaataccg cgggctcgat
    19501 atgaatcaag cgccggatat tattcacgta tccaatggta cggaagcgat acgcatgctg
    19561 gatcgccagc accgttttga cctcatcctg atgactttgc acatcgagga tatccacgcc
    19621 atcgctcttg ccaaaaaaat tcgttcgctt ggccatgaaa tgccgatcat cgttttggct
    19681 tacgataata tcgaaacgca ggaagtaatg acccatcggg atgccgatat ttttgaaaat
    19741 atttttattt
//
LOCUS       DAOQNQ010000028        19687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_958_length_19687_cov_6.123727, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000028 DAOQNQ010000000
VERSION     DAOQNQ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19687
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_958_length_19687_cov_6.123727"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PK754_03400"
     CDS             <1..365
                     /locus_tag="PK754_03400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB08580.1"
                     /translation="GKNGSVTTSWNKSGKIMARYNPAHTIIPDHVELLQNYPNPFNPS
                     TTIRFGLSENGTVRLTVYNLLGQKVKTLVQQHVAAGYHTIVWNGRNESGHAVSSGVYL
                     YRLEIQGHVVTKKMMFLK"
     gene            386..928
                     /locus_tag="PK754_03405"
     CDS             386..928
                     /locus_tag="PK754_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08581.1"
                     /translation="MQRIKLGLTLALLSFLIACEEDPATQVERGWQLFESGDFLEARS
                     AFMNAATSGSTDAYNGLGWSYLRLDSISEARIAFAFASSDSNPEIYAGWSSANVLSED
                     FQKALEKSAVVLRQDANFVFSHDTDVDYKDMIWQRAYCYYHLGQYALSLQEIQKISPD
                     FNSVNPSDILNKLESLSNEL"
     gene            1122..2906
                     /gene="carB"
                     /locus_tag="PK754_03410"
                     /pseudo
     CDS             1122..2906
                     /gene="carB"
                     /locus_tag="PK754_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
     gene            2903..3304
                     /locus_tag="PK754_03415"
     CDS             2903..3304
                     /locus_tag="PK754_03415"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HNB08582.1"
                     /translation="MKPGSGPIVFFDGVCNLCNHAVDFIIRHDTPHRIRYASLQGETA
                     ASHLPLEIRSSLSTLVLFHKEQIFTHSTAVINIARLMGWPWKMAGVFFIIPRFLRDAL
                     YTVIARHRYLWWGRRPTCRIPSVEERQLFLP"
     gene            3312..4388
                     /locus_tag="PK754_03420"
     CDS             3312..4388
                     /locus_tag="PK754_03420"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNB08583.1"
                     /translation="MRFEVLPAHNPAVRERWNAFASSVDGSSVLQSYEWGEVKRGLWQ
                     PFYTAVTDETGQIILAALILKRPMPLVGRAIFYAPRGPLFRELNAALLKAFADGVCAL
                     AKKENVFALRCDPEIEETNTSILSVFQNAGFSRNPENIQPRGTILLDIRPGEDDLLKS
                     FHHKTRYNIKLAEKKGVTVEEINSSDGIDIFYDLFEITSARDKFMILRRSYFQHLHKT
                     LSDSGLSTVFVAKYDGKPLGAVILTTFGKRMVYLYGASSNAHRNLMPNHLLHWRAILW
                     AKERGLTTYDFWGVPANPTETSPLWGVYRFKKGFNETETRWMGTYELVFNRFWYFVFE
                     KAAVAFKTAIRFLKTGKIKSSVGD"
     gene            complement(4398..4895)
                     /locus_tag="PK754_03425"
     CDS             complement(4398..4895)
                     /locus_tag="PK754_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNB08584.1"
                     /translation="MTEEPKKDEIHKAFRAYLISHSLKATPERFAILDEIYATNFHFD
                     ADDILMRMRKKNSMASRATIYRTLELLEKCGLIRKAKLGDTKAYYEHTYGRHHHEHMK
                     CTSCGRVIEFESEEIEKLQDVICRQYNFKMTHHILHLFGICEECQQSPDRKPKQTKLS
                     ELTVK"
     gene            5202..5984
                     /locus_tag="PK754_03430"
     CDS             5202..5984
                     /locus_tag="PK754_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HNB08585.1"
                     /translation="MSGEIVQKQKEALLKKIAEHKALSKPVLDKVRKASSLEVVIMFD
                     TTGSMDTYLSEVQVHVQLIVREIRKRVPDARLSVIVYKDHEQGPYVTKTLPLTDKEED
                     VISFLRSPAIEPGAGGGGPEAVECALYEANQLDWSPGNKGKAIILIGDKPPHGVMDGF
                     EECTSNRDYRIETNRLIEKHVKVYTVLCNNVAETRANFQWLADKTGGKFVTLEAIHDL
                     VNLIVAVSIKESNPLLLQAYTEELRQKGILTDSKRKLLESIR"
     gene            5968..6972
                     /locus_tag="PK754_03435"
     CDS             5968..6972
                     /locus_tag="PK754_03435"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB08586.1"
                     /translation="MKVFDNMTQDPIFAESSNENNDNVLPKSRILIVEDDTAVRNMVT
                     EFLSKRSFEIREATNGVEGLRIAMTEKPDAILVDIIMPEMDGLEMIRQLRQHPGTMLI
                     PVIVMTAVSNLETKINVFRAGGDGLLQKPFDLQELEVRIDRAIQISNNFMKLTYVDAL
                     TGVYNRRLFDDRLPVELNRSKRYNQPLALVMVDIDHFKKFNDTYGHRAGDFVLNAVGQ
                     TIKKSLRTQDIVCRYGGEEFCIIMPMTLSKDAAMVMSRIRSDFQDRYFHSPYDKTDFN
                     VNISIGVAQYPNDSSEGDNLVRCADEALYEAKETGRNRVVIYNPASTVFKNRVSKLQF
                     "
     gene            complement(7007..8008)
                     /gene="purM"
                     /locus_tag="PK754_03440"
     CDS             complement(7007..8008)
                     /gene="purM"
                     /locus_tag="PK754_03440"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNB08587.1"
                     /translation="MSKVITYKDAGVDIAAGNSTKDQIKKMVRKTYNANVLSEIGLFG
                     GFYRFPKSKYKDPVLVSSADGVGTKLKIAVMTGQHDTVGEDLVNHCVNDILACGAKPL
                     FFLDYLGLGKVHPDVIRNIIIGLTRGCKNNGCALIGGETAQMPGFYADDEYDMAGTII
                     GAVERKNMINGRTIRKGDIMIGLYSSGLHTNGYSLARKVLLEKYKLDQYVEELGCTVA
                     EELLKTHKSYLKPVSAVMDKIQIKGISHITGGGIVENTERIIPKKLKMHMDWSAWQEL
                     PVFGLIRRLGNVPHDDMVRTFNLGVGMILIVSPKDVTKTMQILKSKKEKPFVMGEIG"
     gene            complement(8094..11204)
                     /locus_tag="PK754_03445"
     CDS             complement(8094..11204)
                     /locus_tag="PK754_03445"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB08588.1"
                     /translation="MKKVFLFCVLCVVYGVHGQSNLQRAFTSKQEFASKNKTSGTLRV
                     LALRVQFKADNSGQTTGNGRFDLRDLRDSLRIDPAPHDKRYFEDHLLSMRNYFYDVSD
                     GALDITYTVKPNGDTAAYHLSRTMEYYGRIVSRDERDELLAELLHDAITTADTTDGID
                     FSQYDHVIVFHAGVGQDFSLEDATPRDIPSRFVTPSVVDQKYSGGISVNNGAHRVQSG
                     IIVPETESQFFTDAVLGVEVFREIGLNGILIANFGSALGMPDMFNTDQGTPGIGVFGL
                     EDQGAVNGDGMIPAEPDPWTKIYMGWAQPIVVTDTIHAQLQSRKTAGHSVILKVPVSA
                     SEYYLIENRQRDVISNTLSPETLIRIDTIYNVDNTIDEIDTAYMTGVERSPVSGVITK
                     VDEYDSALPGSGLLIWHVDENVIAANLSDNAINNNIERRGIRVVEGSGSQDIGRNFQS
                     FFGTTVGAGDKYDFFFHGNEGFGFYNKKTDSVFFDSKSVPAALTYDRASTGVRITGIS
                     VRQPVMAFDLQSSLQQNGFPQYTGVTIPQAGLTWGNIQGDAKKEIIAVSEKGLVFAWQ
                     ADGSEVIDHDQTITDHRLGQDSVQFAYAWISDFHDSVTGGPAVSDLDQDGYDEIIIAT
                     KNGKIFALRGADGNNDKIADTLFVYLTGQTITTSPMVLPDRRIVVGTSTGDIIILHPN
                     GVFDATLALSSAIRAIVRLENDYLVVATGQHIAKVNLNNMTFTSDAYVPASGSVWLVA
                     GDMDQNGVNSIIAANGKNIHPFYSEPAFTYELPSVISAPPVIGDIDGDEYPDIVFAVE
                     NFVYALNHNGTLVNNFPVRLSDAYRVDQIISSPLLADIDNDGQCDILIAGSDGLIYAV
                     NGRGEKIGGFPLTIGDQAIASPMITDMDGDFDLEMVSVSAEGYVYNFDLPATLTLQTL
                     KWPKYGRDLSLSHLSNESNTPDSVSTGGLMPARSVYNYPNPTRGERTVIRYFLSESAK
                     VTIKIFDLAGDLVTTLHGPGLAQTDNEVVWNLDRVQSGVYFAKVQAKTSSGKKEIRTV
                     KIAVTK"
     gene            complement(11290..12399)
                     /locus_tag="PK754_03450"
     CDS             complement(11290..12399)
                     /locus_tag="PK754_03450"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNB08589.1"
                     /translation="MQVSRVKNSNIVVAVFLMFVCSFETQAQTSEAGVLFLLVPPGAR
                     HNGMGQTGVSTANDANAIYYNPGALGFIATQETPRDVQFMHVNWLPQFNLSDLFYDYG
                     SIVMFLPDIGVVGYNFTYFNIGEQQRTNAAGDDLGTIHSYELSTGVTYATKMTEDWGI
                     GGNFKFIYSSLDNKKGTDNNTAEVQNGVGSSVAIDLGVMKKNFFVEDLTFGASLVNVG
                     PSIAYADEDQADPLPTNLRLGVSYPAYKSEFNNVLVAYEINRQIVRGRSTGSDPLISG
                     NSKFFTRSAIFTTWADNGWHRLGHNIGAEYTYSDFVAMRTGTSLDFAGKLYDLTFGVG
                     IRYDVFKIDFAYSTGLTERFNARDGSQFYSIGVRF"
     gene            12789..13466
                     /locus_tag="PK754_03455"
     CDS             12789..13466
                     /locus_tag="PK754_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08590.1"
                     /translation="MFRIVMTLKKEIHRKFFHFSAVIIPIGYYLTDYATALWCIGALL
                     GIAIVTEYLRLYIPSFSALFNRFFGSMLRDGETDHYSGATFLLIASFLVIFAFHKDVA
                     VLCLLFLIFGDGMAALIGKTIGRTRLLGLDKTLEGALACFAVCLGISFFFGHLPLSVR
                     IAGAFAATVAEIMPMRTSDNLRIPIISGSIMQLMVIQIQQKQPIYLEEKEFVLSFINY
                     FNLFGNV"
     gene            complement(13463..14854)
                     /locus_tag="PK754_03460"
     CDS             complement(13463..14854)
                     /locus_tag="PK754_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNB08591.1"
                     /translation="MILIDGARYDVFERLLAEGRLPAIQEHIVARGAYRRMTTVFTST
                     TGPAYVPFLTGCFPGKANLPGIRWFDKTAFAEKPFWNPHRFRSYCGWEGFFMNRDINP
                     NIKTIFDLTENPINIFGPITRGILKNNNRGPLKKAWMIARAHSNGVYEPVDDAAFEVC
                     MKTLDEPSAFRFLALPGVDGISHNTFPHHERVLAAYQRADRGIAAIVKKLKSVEQYDQ
                     TVIAICSDHGLSQTHTHFDVPVYMETSLGFKTLFYTNIFRWNPDASAQVSGNGMVHIY
                     FKNGDWRKSCFYEDIIRFPINPVDDFLKHEAVDLVITRMQNGEARVDSRRGTGFIKEE
                     NERILYRVEGKDPFGFEVLPPSMSRNEALARTFETNYPDALVQIVQNFHSPRCGDMML
                     SATIGYDLRFRWENPEHKSSHGSLHREHMMTPFCWSVPITTDHVRTVDVFPSMLKILG
                     KDIPEGVDGVSFV"
     gene            complement(14962..15366)
                     /locus_tag="PK754_03465"
     CDS             complement(14962..15366)
                     /locus_tag="PK754_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08592.1"
                     /translation="MRLFLNGIFVFILLNLVACTATQQTSYKRKEITMDLLRERFLDK
                     KFAVRMQFYISREELIEETGYLKSAESDKAVLFEYYDARDKQYRNLEVPIQNINTIEL
                     ASKGSDRFSKTQKVAMGSAIVMALLLVFAISI"
     gene            complement(15379..17625)
                     /locus_tag="PK754_03470"
     CDS             complement(15379..17625)
                     /locus_tag="PK754_03470"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNB08593.1"
                     /translation="MISKEEVLEFHAQGRKGKIEVVPTKPCSTQRDLSIAYSPGVAVP
                     CLEIEKNPEDAYNYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKRFADI
                     DVFDIELDTKKVDEIIRVCQILEPTFGGINLEDIKAPECFEIEETLKKTMNIPVFHDD
                     QHGTAIISGAALLNATELVGKRMDEVRVVVNGAGASGIACANFYISLGVKPENLILCD
                     TKGVVYKGRKEGMNKYKEVLANDTPLRTLADALKGADVFAGLSVANVLTQDMVKTMAR
                     DPIIFAMANPDPEITYDDAKAARPDAIVATGRSDYPNQVNNVLGFPFIFRGALDVRAT
                     TINEEMKRAAAMALAKLAKEDVPDSVSKAYGDERLRFGREYVIPKPFDYRVLIWEAAA
                     VAQAAMESGVARIKVNIEEYKELLERRLGKSREVMRTMIHTARKHPKRVVFPEGAHEK
                     ILRAAHIILEEGIAKPILLGRRELIEKTAAELGVNISGAEIIHPSTSDNYLSYVEKYY
                     AMRQRKGATLTEARNAIRNYTTYGSMMVRLRAADALIAGVSQHYPDTIRPALQIVEME
                     EGISSVSGLYMLIIKNQIYFIADATVNIDPSVEELAEIAIVSARVARRFNTEPRVAMI
                     SFSNFGSARHPHAEKVRKAVEIVRQREPKLMVDGEMQADTAVDATILEKDYPFSTLKG
                     GANVLVFPELQSANAAYKLLQKLGGAETIGPILMGLRYPVHVLQRGCEVNDIVNMTAI
                     AVVEAQEK"
     gene            complement(17650..18180)
                     /locus_tag="PK754_03475"
     CDS             complement(17650..18180)
                     /locus_tag="PK754_03475"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB08594.1"
                     /translation="MRIVLLLAALNLSASLYAQDKKTPSSGFRAEFLMHLDQMEKKYT
                     DMANTIPADKYGFRPAEGVRSIGEVLMHCAGGNYFLLGFIGVKSDTPMNENFEKNADP
                     KKATETIKASFALVRNTVLNMNDADMDKTVKFFGGKDATIRNVLFTTMNHQHEHLGQL
                     IAYARANGITPPWSAN"
     gene            complement(18405..18923)
                     /locus_tag="PK754_03480"
     CDS             complement(18405..18923)
                     /locus_tag="PK754_03480"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR/HmsC family protein"
                     /protein_id="HNB08595.1"
                     /translation="MMRHVVLVSSYLINFIIFISLTHAQEIQVPLHVQSQFYKKIFAY
                     SLTIPNSSNPTIAIIAPSHDAGENVKAAFAKSGMTEVNVLTSQEFLASSKRYDVAYLC
                     AGVRTSEVSAFCQGKKILTVTGSVEVYDAGKISIGLKLREGKCQILLNMDEVKKEGHR
                     FSAAFLNLTKSK"
     gene            complement(19019..>19687)
                     /locus_tag="PK754_03485"
     CDS             complement(19019..>19687)
                     /locus_tag="PK754_03485"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNB08596.1"
                     /translation="YMIGELPCLEMLDLGHNEVNEIPDEIGDLSTLQFLYLTDNNLTD
                     FPQTLKRLISLRYLNISENSFSGLPTSIGELTSLIELRASDNCIENMDIRWSGLTALR
                     ELHLQNNQLTKLPDGLGNLSLLRKLFLQNNELKKLPSALAKLKNLAELNLRNNSLYEL
                     PKMIGDLVRLTDLDLRQNHLKDLPESLTRLPHLYKLDLRWNEIKENNSIVEALREKGC
                     VVYT"
BASE COUNT         5365 a         4609 c         4300 g         5413 t
ORIGIN      
        1 aagggaaaaa cggatccgtg acgacgtcat ggaacaaatc gggtaaaatc atggctcggt
       61 ataatccggc tcatacgatc attccggatc atgttgagtt attgcaaaac taccccaacc
      121 cgtttaaccc gtccacgacg attcgtttcg gattatctga aaacggtacg gtacgtttga
      181 cggtgtataa tcttctcggg caaaaagtaa aaactttggt gcagcaacac gtggccgccg
      241 gatatcatac catcgtatgg aacggacgca acgaatccgg tcatgccgta tccagcggcg
      301 tgtacctgta tcgtcttgaa atacaggggc atgtcgtaac aaagaaaatg atgtttttga
      361 aataaaaatt taaggataca tatctatgca acgcataaaa ttaggattaa cgctggcgct
      421 tctttcgttt ttgatcgcat gcgaagaaga cccggcaaca caagtcgaac gtggatggca
      481 attatttgaa agcggcgatt ttctggaagc ccgttcggca tttatgaatg ccgcgactag
      541 cggaagcacc gacgcttaca acggactcgg gtggtcttat ctacggctgg attcgatttc
      601 agaagcgcgc atcgcgtttg cttttgcgtc atcggattcc aatccggaaa tttacgccgg
      661 ttggtcatca gccaacgtat tgtcggaaga ttttcaaaag gctcttgaaa aatcagccgt
      721 cgtgctcagg caggatgcta attttgtttt ttcacatgat accgacgtgg actataaaga
      781 tatgatctgg caacgcgcct attgttatta tcatcttgga caatacgccc tgagtcttca
      841 ggaaattcaa aaaataagtc cggattttaa ttccgtcaat ccttcggata ttctgaataa
      901 actagaatcc ctgtcgaacg aactgtaact ttttttagaa accggtaatt tgatgctttg
      961 gagatcaacc ctcaggccca aattaccggt ttttttctat gatacgccct atcggctcat
     1021 tccgcacaga ttataaaatc ccttgaaatg aaggctcgtt atctttagtt tgcgccggtc
     1081 taactaaaag caggtacccc ttgacatctc ttgaaatatt gatccaaaaa atctcccgcg
     1141 cgcttctgat cgaagccaaa caaaaaggat tttccgatcg tcagttggca cacatctggg
     1201 aaacggacga aaaaaccgtt cgccggttgc ggcacgaatt gaaggtattt cccgtataca
     1261 aaaccgtaga tacctgcgca gcagaatttg aagcggagac cccttacttt tattccacct
     1321 acgatcagga aacagaaaac gcgccgtcgg ataaaaagaa ggtgatcata cttggcggcg
     1381 gacccaaccg tatcggacaa ggtatcgagt ttgactattg ttgtgtacat ggtgtatatg
     1441 cattgcagga agaaggttat gaagtcatca tggtcaactg caatccggaa actgtaagca
     1501 cggattacga tacatcggat cgtttgtatt ttgagccgct gacatttgaa gatgtgatga
     1561 atattatcga atccgaaaaa cccgacggtg tgattgtgca gtttggcgga cagacgccgc
     1621 tcaagttggc catacctctg atgcaagccg gtgtaaaaat tcttggtaca tcaccggaag
     1681 atattgatgt cgccgaagac cgtaaaaaat tcggcgcctt actcaatcgt ctaaacatca
     1741 aatgcccgga ttacggcacg gcgtcttcgt ttgaagatgc ccgcacgatc gcccatcgca
     1801 tcggctaccc cgtattagtc cggccttcgt atgttctcgg cggtcgtgct atggaaatcg
     1861 tttatgatga taccgcactg gaaaactaca tggcacgagc catcaacgtg tcgccggagc
     1921 atccgattct cgtagataaa tttctggtag acgcgtacga atttgacgtg gatgccattt
     1981 ccgacggaaa aaccgtagtg atcggcggca tcatgcagca tatcgaagaa gccggcattc
     2041 attccggtga cagctcctgc gttctaccgc cgtattcggc ttcggacgaa gtactggcca
     2101 aactgagagc ctacacagtt cagctcgcac gcgaattgaa tgtcatcgga ctgatcaata
     2161 tccaatacgc actcaaaggt gaagaagtat atgtattgga agttaatccg cgtgcttcgc
     2221 gtaccatacc ctttgtcagt aaagcgatcg gcgtaccctt ggctaaacta gccgcgaaag
     2281 tgatggttgg aaaaacgtta gaatcattaa attacacaaa cgaaattatt ccgccgttta
     2341 cctccgtcaa agatccggtc tttccttttg ccaagttccc cggtattcgc atgtacctga
     2401 gcccggaaat gcgttccacc ggcgaggtaa tgggaattgc cgaaactttt cctcaagcct
     2461 tagctaaagc gcacattgcc gccggtatgc aaatgcctac gtcgggcgga gtttttgtca
     2521 ctgtcaacga ccgggacaaa tctaaaattt ctctgattgc caaagaattc atggatctgg
     2581 gattcacgat ttatgccacc gaaggcacgg gcaaagttct ggaacaatca ggcatcgaat
     2641 gtaaacatgt attcaaaatt catgaaggcc gtcccaacgt agttgaccgc attaaaaaca
     2701 aagaaataca catcgtcatc aacacaccga caggcaaagc cgcaaaatta gacgaacgct
     2761 acatcggcga agcggccatg ctgtacaaaa taccgatgat cacgacgttg ccgggcgccc
     2821 tggggcttgt caaagggttg cgcgccatca aagaacaaaa gctagatgtg cgcagcatcc
     2881 aagaacatca tacggcgctg gtatgaagcc gggctccggg ccgatcgttt tttttgatgg
     2941 ggtatgtaat ttgtgtaatc atgctgtgga tttcataatc aggcacgaca caccgcatcg
     3001 catcagatac gcttccctgc aaggcgaaac ggcggcatca catctaccct tggaaatacg
     3061 atcctcgctt tcgactttgg ttttatttca caaagaacaa atattcacgc actctacggc
     3121 cgttatcaat attgcccgtt tgatgggttg gccgtggaaa atggctggcg ttttttttat
     3181 catccctcgc tttttgcgtg acgcgctcta tacggtcatc gcacgccatc gttacctgtg
     3241 gtggggacgt cgcccaacgt gcaggatacc gtcggtcgaa gaacgtcaat tatttctacc
     3301 ttgagtcata catgcgattt gaagtattac ccgcccataa tcctgccgta cgtgaacgat
     3361 ggaacgcttt cgcatcatcg gttgacggtt cgtccgtttt acaaagttac gaatggggtg
     3421 aagtaaaacg cggtttatgg caaccgtttt acacggcggt taccgacgag accggtcaga
     3481 ttatccttgc ggcactgata ctcaaacgcc caatgccgct tgtcggtcgc gccatatttt
     3541 atgcaccgcg cggaccgctt tttagagaat taaacgccgc acttctaaag gcattcgccg
     3601 acggtgtctg cgctttagct aagaaagaaa atgtttttgc attgcgatgt gatcctgaaa
     3661 tcgaagaaac caacacttcg attttatctg tttttcaaaa tgcaggattt tcaagaaacc
     3721 cggagaatat ccaaccgcgc ggtacgattc ttctggatat tcgtcccggt gaagacgatc
     3781 tgctcaaatc ctttcatcac aagactcgat acaatatcaa attagcggaa aaaaaaggcg
     3841 ttacggtaga agagattaat tcttccgacg gcattgatat tttttacgat ttatttgaga
     3901 tcaccagtgc gcgggataaa tttatgatac ttcggcgttc gtatttccaa cacttacata
     3961 aaacactttc cgatagcggt ttaagtactg tatttgttgc caagtacgac ggtaaacctt
     4021 tgggcgctgt gattttgact accttcggaa aacgcatggt ttatttatac ggcgcttcat
     4081 ccaacgcaca tcgcaacctt atgcctaatc atttattgca ttggcgcgcg attctttggg
     4141 ctaaagagcg cgggttgacg acatacgatt tttggggcgt tccagccaac ccgaccgaaa
     4201 ccagcccgct gtggggtgtg taccgtttta aaaaaggatt caacgaaacg gaaacgcgct
     4261 ggatgggtac gtacgaattg gtctttaacc gattttggta ttttgtcttt gaaaaagctg
     4321 ctgtggcatt caaaaccgcc attcgatttt tgaaaaccgg aaaaataaaa tcttcggtcg
     4381 gggattaaaa aaaagtttta tttaacggtg agttcggaaa gtttggtttg tttgggcttg
     4441 cgatccggcg attgctgaca ctcttcacag ataccgaata gatgcaaaat atgatgcgtc
     4501 attttgaaat tatattgacg gcaaatcaca tcctgtaatt tttcgatctc ttccgattca
     4561 aattcgatca cgcggccgca cgacgtacac ttcatgtgct catggtggtg gcgcccgtaa
     4621 gtatgttcat aatacgcttt ggtatcgcct aacttggctt tgcgtatcaa tccgcatttt
     4681 tctagaagct ctaaggttcg atatatcgtt gcacgcgaag ccatgctatt tttttttcgc
     4741 atccgcatga ggatgtcatc ggcatcaaaa tggaaattgg tagcgtatat ctcgtctaag
     4801 atcgcaaaac gttcgggcgt agcttttaat gaatgcgaaa tcaggtaggc gcgaaacgct
     4861 ttgtgaattt cgtctttttt aggttcttct gtcatatgca cgatgaatga cgctttcttg
     4921 tctgattctc gtatcgtgtt tgatagtata cccgcaacat gcccttttat aataattttt
     4981 ctgcaatagt tcaataccgg ttcgcggttt tttccgttta ttacaggttt tacaattttt
     5041 tggatacgag gcctttcgaa ggcgtcatcg gataaaaaaa agaacgtgtt gccgattttt
     5101 taaggctcca tgagacgatt ttatcttaaa atttttgttt gtctgtgcat ggcttttcgt
     5161 gtatattcga aaatcatttc ccgaacactt caaatgcagc tatgagcggc gaaatcgtac
     5221 aaaaacaaaa agaagcgctc ttaaaaaaga tcgcagagca caaagcgcta tccaagcctg
     5281 ttttggataa agtgcgaaaa gccagttcgc ttgaagtggt gattatgttt gatacgacgg
     5341 gcagtatgga cacgtacttg tccgaagtgc aggttcatgt ccagctcatc gtgcgtgaaa
     5401 tccgtaaacg agtacccgat gcacgtttat ctgttatcgt ttataaagat cacgagcagg
     5461 ggccatacgt taccaaaacc ctgccgctga cggataaaga agaagatgtg atttcatttc
     5521 tacgttcccc ggcgatcgaa cccggtgccg gtggtggcgg tccggaagcg gtcgaatgcg
     5581 cactatatga agccaatcaa cttgattggt cacccggaaa taaaggaaaa gctatcattt
     5641 tgatcggcga taaaccgcct catggtgtga tggatggttt tgaagaatgt acgagcaatc
     5701 gggattaccg aattgaaacc aaccgcctta tcgaaaagca tgttaaagtg tacaccgtgc
     5761 tgtgtaataa tgtcgcggaa acgcgtgcca attttcaatg gcttgccgac aaaaccggcg
     5821 gtaagtttgt gacattggaa gccattcacg acttggtcaa tctgatagtg gccgtgagca
     5881 tcaaagaatc caatccgctt ctgcttcagg cttataccga agaactgcgg caaaaaggta
     5941 ttctcacgga ctcgaaaaga aaattacttg aaagtattcg ataatatgac acaagatccg
     6001 atctttgccg aatcctccaa cgaaaacaat gataacgtgc ttcccaaaag ccgcatccta
     6061 atcgtcgaag acgatacggc tgtgcgcaat atggttacgg agtttttgag taaacgttcg
     6121 tttgaaattc gagaagctac caatggtgtc gaaggtctcc gtattgccat gacggaaaaa
     6181 cccgatgcga tactcgttga tatcattatg ccggaaatgg acggacttga aatgatacgc
     6241 cagttgcgtc agcaccccgg tactatgctt atccccgtta tcgtgatgac ggccgtcagt
     6301 aatctcgaaa ccaaaataaa tgtattcaga gccggcggcg acggccttct gcaaaaaccg
     6361 tttgatttac aggaactcga agtgcgtatt gatcgcgcca ttcagatttc caataatttc
     6421 atgaaactaa catacgtgga tgctttgacg ggtgtgtaca atcggcgctt gtttgacgat
     6481 cgtttgccgg tcgaactgaa ccgctccaaa cgttataatc agcctctggc gctggtgatg
     6541 gtggatattg accacttcaa aaaattcaac gatacgtacg gacatcgtgc cggcgatttt
     6601 gtgctcaatg ccgtcggaca aaccataaaa aaatcattgc gaacacagga tatcgtgtgc
     6661 cggtatggcg gagaagagtt ttgtataata atgcctatga cgttgagtaa agatgcggcg
     6721 atggtcatga gccgcatacg atccgatttt caggatcgtt attttcactc gccgtacgat
     6781 aaaacggatt tcaacgtcaa tatcagcatc ggcgtagccc agtatcccaa tgattccagc
     6841 gagggcgata acttggtacg atgtgccgac gaagctttgt acgaagccaa ggaaaccggt
     6901 cgcaatcgcg tggtgattta taatccggct tcgacggttt tcaagaatcg tgtaagtaaa
     6961 ctacagtttt aatttttcga taccttcacc ggggttgatt tttcgatcaa cctatttccc
     7021 ccatcacaaa cggtttttct ttttttgatt tcaatatctg catcgtcttt gttacgtctt
     7081 tagggctcac gatcagtatc atgccgacgc cgagattaaa tgtacgcacc atatcgtcgt
     7141 gcggtacatt gcctaagcgg cgaatcaatc cgaaaacagg taactcctgc caggccgacc
     7201 aatccatatg cattttcagt tttttcggaa tgatgcgttc cgtattctct acaataccgc
     7261 cgcccgtaat atgagaaata cctttgattt gaattttatc catcactgca gaaacgggtt
     7321 tcaaataact tttgtgcgtt ttcaataatt cctcagctac cgtacaaccc aattcttcca
     7381 catattgatc cagtttatac ttttccagta aaaccttgcg ggccagcgaa tagccgttag
     7441 tatgcaaacc actggaataa agtccgatca tgatatcgcc tttgcgtatc gtgcggccat
     7501 tgatcatgtt ttttcgttcg acggcaccga tgatcgtacc cgccatatcg tattcgtcat
     7561 ccgcataaaa cccgggcatt tgcgccgttt cgccgccgat aagtgcacag ccgttatttt
     7621 tgcagccgcg cgtcagaccg atgatgatat tgcggatcac atccggatgt acttttccaa
     7681 gcccgagata atccagaaaa aatagcggtt tggccccgca agctaaaatg tcgttcacac
     7741 aatgattaac caaatcttcc ccgacggtat cgtgctggcc ggtcatcacg gcaattttta
     7801 atttggtacc cacgccatcc gcgctcgaaa ctaatactgg gtctttatat ttactcttgg
     7861 gaaaacgata aaaccctccg aataggccaa tctccgaaag tacattggcg ttgtacgttt
     7921 ttcgaaccat ttttttgatt tgatctttgg tcgaattacc ggcggctata tctacaccgg
     7981 catctttgta tgtaattact ttggacaaaa aattatctcc atctgtgtaa aaaaactctc
     8041 gttgcgggat tatacaaaaa agccaatacc aaaaacaacg atcaaaaaaa ctcttatttg
     8101 gtcacggcga tttttaccgt acgtatttcc tttttcccgg aagacgtttt agcctgtact
     8161 tttgcaaaat atacgccgct ttgaacgcga tccagattcc aaactacttc attatccgtc
     8221 tgagccaaac cgggaccgtg aagtgtcgtg actaaatcac cggccaaatc aaaaattttg
     8281 attgttactt ttgccgattc cgaaaggaag taacgtatta ccgttcgttc accgcgcgtc
     8341 ggattgggat aattataaac cgatcgggcc ggcataagcc ctcccgtcga gaccgaatcg
     8401 ggcgtattgc tttcattact caagtgacta agcgaaagat cgcgtccgta ctttggccat
     8461 tttagtgttt gtaaagtcaa cgtcgccggt aaatcaaaat tatacacata cccttcagcg
     8521 gaaacggaaa ccatttccaa atcaaaatct ccgtccatat ccgttatcat aggcgacgcg
     8581 attgcttgat caccgatcgt gagcggaaaa ccgccgatct tctcaccacg accattcacg
     8641 gcatagatga gcccatccga gcccgcaatc aaaatatcgc attgcccgtc attatcaata
     8701 tccgctaaaa gcggtgagga aatgatctga tccacccggt atgcatcact gagccttacg
     8761 ggaaaattat ttaccaacgt gccattatgg ttgagtgcat atacaaagtt ttctacagca
     8821 aaaacaatat ccggatattc atctccgtct atgtcaccga tcacaggcgg tgccgaaata
     8881 acggaaggta attcatacgt aaacgctggt tccgaataaa acggatgtat gttttttccg
     8941 tttgctgcga taatggaatt gacaccattt tgatccatat cgcctgctac cagccaaaca
     9001 gaacctgatg cagggacata tgcgtccgac gtgaatgtca tattgttcaa attgactttc
     9061 gcaatatgtt gtcctgtcgc tacaaccaaa taatcgtttt ccaatcgcac gattgctctt
     9121 atcgccgaac tcaacgccag tgtagcatca aaaactccat taggatgtaa gataatgata
     9181 tcccccgtcg aagttccgac gactattcgc cggtcgggta ataccatagg ggaagttgtg
     9241 atcgtctgac cggtaagata cacaaataaa gtatcggcta ttttatcgtt attaccatca
     9301 gcgccacgca gggcaaaaat tttaccgttt ttagtcgcaa tgatgatttc atcataacca
     9361 tcctgatcca gatcagatac cgccgggcct ccggttacag aatcatgaaa atccgaaatc
     9421 cacgcgtacg caaactgaac tgaatcttga cccaaacgat gatccgttat cgtctgatcg
     9481 tgatctatca cttcagatcc atctgcttgc caagcaaaga ccaaaccctt ttcactaacc
     9541 gctatgattt cttttttggc gtcgccttga atattacccc acgtcaaccc ggcttgaggt
     9601 atggtaacgc ccgtatattg aggaaaccca ttttgttgaa gcgatgattg cagatcaaat
     9661 gccatgaccg gttggcgcac actgataccg gtgatacgta cgccggtcga tgcgcgatcg
     9721 taagtgagtg cggccggtac agatttagaa tcaaaaaaaa ccgaatctgt ttttttatta
     9781 tagaaaccaa acccttcatt gccgtgaaag aaaaaatcat acttatctcc ggcgccgacg
     9841 gtcgttccaa aaaaactctg aaaattgcgc ccgatatcct gcgaaccgga tccttccact
     9901 acgcgaatac cgcgacgttc gatattatta ttgatcgcgt tatcactgag attagccgcg
     9961 atgacatttt catccacatg ccatatcagc aaaccacttc ccggcaaagc gctgtcgtat
    10021 tcatccactt tggttattac gccgctgacg ggtgagcgct cgactcccgt catgtaagcc
    10081 gtgtctatct catcaatcgt attgtccaca ttatatatcg tgtcaattcg aattaatgtt
    10141 tccggtgaca gcgtattgga aataacgtca cgttgcctgt tttctatgag atagtattcg
    10201 gatgccgaaa caggaacttt taatattacc gaatgccctg ccgttttacg agattgcagt
    10261 tgcgcatgga tagtatcggt aacaacgatg ggttgcgccc aacccatata tattttagtc
    10321 catggatcag gttcagcagg gatcataccg tcaccgttga ctgcaccttg atcttcaagg
    10381 ccaaaaacgc caatgccggg cgttccctga tcggtattaa acatatccgg catgccgagc
    10441 gcactcccaa aattggcaat caaaataccg ttaagcccga tttcacgaaa aacttcaaca
    10501 cctaaaacag catcggtgaa aaattgactt tccgtttccg gaacgatgat accggattgg
    10561 acgcgatgcg caccgttatt gaccgaaatt cctccgctgt atttttgatc aacgacagac
    10621 ggtgtcacaa aacgtgaggg tatatcacga ggtgtcgcat cttccaagct aaaatcttgt
    10681 ccgactccgg catgaaaaac aataacgtga tcgtattggg aaaaatcaat gccatccgtg
    10741 gtatcggccg ttgttatcgc atcatgcaaa agttcagcca aaagttcgtc gcgttcgtcg
    10801 cgggaaacga tacggccata atactccatc gtccgtgaaa gatgatatgc ggctgtgtcc
    10861 ccattcggct ttacggtata agtaatatcc aacgcgccgt cggatacatc ataaaaataa
    10921 ttgcgcatac tcagcaaatg atcttcgaaa taacgcttat cgtggggcgc cggatcaatg
    10981 cgtagcgaat cacgcaggtc tcgcaaatca aaacgaccat tccccgtggt ttgtccgctg
    11041 ttatccgctt taaattgaac ccttagcgct aaaacacgga gcgtaccgct cgtcttgttt
    11101 ttggaagcga actcttgctt ggatgtgaag gcgcgttgca ggttggactg cccatgaact
    11161 ccgtaaacaa cacacagaac gcaaaacaaa aaaacttttt tcatgatttt tcagaaatat
    11221 tgatagatat aaaaaagccg aattcacgcg cggagaattc ggctctgaac atacattgaa
    11281 aaattgatat cagaaacgaa cgccgatgct ataaaactgc gatccgtccc tcgcattgaa
    11341 gcgttccgtc aaacccgtag agtatgcaaa atcaattttg aatacatcat aacgaatacc
    11401 gacaccaaac gtcagatcat aaagtttacc ggcaaaatca agtgatgtac ccgttcgcat
    11461 agctacgaaa tccgaatagg tgtattcagc tccgatgtta tgacctaaac gatgccatcc
    11521 attgtcagcc catgttgtaa agatcgccga gcgtgtaaaa aacttagaat tgccgctgat
    11581 caaaggatct gaaccggtgc tgcgtccgcg tacaatttgg cgattgattt cgtaggcgac
    11641 aaggacatta ttaaattcgg atttgtaggc cggatacgac acacctaaac gcaaattagt
    11701 cggtaaggga tctgcttgat cttcatccgc ataagcgatg ctcgggccga cattgacaag
    11761 gctcgccccg aaggtaaggt cttcaacgaa aaagtttttt ttcatcacac caagatctat
    11821 cgcaacggaa ctaccgacgc cgttttgaac ttcagccgta ttattatctg tacctttttt
    11881 attatcaagg ctgctataga taaatttgaa attgccgcca ataccccaat cttcggtcat
    11941 ttttgtagcg taggtcactc cggtggataa ttcgtagcta tgaatcgttc caagatcatc
    12001 accggcggca ttggtacgct gttgttcacc gatattaaaa taggtgaagt tgtatccgac
    12061 aacaccgata tccggcaaga acataacgat agaaccgtag tcataaaata gatcactgag
    12121 gttgaactgc ggcaaccaat tgacgtgcat gaactggaca tcgcgtggcg tttcttgcgt
    12181 tgcaataaat ccgagtgcac cgggattgta gtaaatggcg ttggcatcgt tagccgttga
    12241 tacgccggtt tggcccatac cgttgtgacg cgcgcccgga ggtacaagca aaaagagaac
    12301 tcccgcttca ctcgtttggg cttgtgtttc aaaagaacaa acgaacatca aaaaaaccgc
    12361 tacaacaatg ttgctatttt tcacacgtga tacctgcata tatttctttt ctccaataag
    12421 attcttagaa gttagtgttg attttccctt tagcaaagtg tactacgata aacctcctgt
    12481 aagataataa atttaaaaaa accaatacaa agaaattttc gtttctttga cgtataacaa
    12541 caaccggaaa taaggctgag tgagttttaa tcagatcgct ttcgcgtcat cgaaaccgta
    12601 gcaattgtct gatcaaaatc tcctttcgta tagagggggt tagggaatca aacacatgaa
    12661 gaatatagga aacacgcatg atgctgtcaa acgatttttt gttcattacg ggttattaat
    12721 ctgccgcaca atcttgtgaa acaccattgt aagtatggtt ctaaagcgta tattaaaaaa
    12781 aacaacttat gtttcgcatc gtcatgactt taaaaaaaga gatccatcgc aaattttttc
    12841 atttttcggc ggtgataata cccatcggtt attatctgac cgattacgct acggccttgt
    12901 ggtgcatcgg cgcgctgctt ggcatcgcga tcgttaccga atatttgcga ttatatattc
    12961 cgagttttag tgcgcttttt aatcgttttt tcggttctat gctgcgcgac ggagaaacgg
    13021 atcactacag cggggctacc tttttactca tcgcttcatt tttggtaatc tttgcatttc
    13081 acaaagatgt cgcggtcttg tgtttgttgt ttttgatatt tggagacggt atggctgcgc
    13141 tgatcggtaa aactatcggc cgcacacgcc ttttaggttt agataaaacg cttgaaggcg
    13201 ctttggcttg ttttgctgtt tgtttgggga taagcttttt cttcgggcat ctgccgcttt
    13261 cggttcgtat cgccggggca tttgcagcta ccgtagccga aatcatgccc atgcgcacaa
    13321 gcgataacct gcgcattccg atcatcagcg gcagtatcat gcaactgatg gtgattcaga
    13381 tccagcaaaa acaaccgata tatttggaag agaaggagtt tgtattgtct tttataaact
    13441 atttcaattt gttcggcaac gtctaaacaa acgacacgcc gtcaacacct tcaggaatat
    13501 cctttcctaa aatttttaac atagacggga agacatcgac cgtacgcaca tgatcggtcg
    13561 ttatcggaac gctccagcaa aagggcgtca tcatgtgttc gcgatgcagt gaaccgtgcg
    13621 acgatttatg ttccggattt tcccaccgaa agcgcagatc atacccgatc gtcgccgaaa
    13681 gcatcatatc cccgcatcgg ggtgaatgaa aattttgtac gatctgcact agcgcatccg
    13741 ggtagttcgt ctcaaatgtt cttgcaagtg cttcatttcg tgacatagac ggcggcaaaa
    13801 cttcaaaacc aaacggatct ttaccttcga cacgatacaa gatacgttca ttttcttctt
    13861 ttataaaacc cgtaccccgc cggctgtcaa cgcgtgcttc tccgttttgc atgcgcgtta
    13921 taactagatc aaccgcttca tgttttaaga aatcatccac cggatttatg ggaaaacgta
    13981 ttatgtcttc ataaaaacac gattttcgcc aatctccgtt tttaaaataa atgtgcacca
    14041 tgccattacc ggaaacttgt gccgacgcat cgggattcca ccgaaaaata ttagtataaa
    14101 acaacgtttt aaaaccaagc gatgtttcca tatacaccgg tacatcaaaa tgcgtatgtg
    14161 tttgcgaaag gccgtggtca ctgcatatgg cgatgacggt ttgatcgtat tgttcgacgg
    14221 actttagttt tttgactatg gccgcaatgc cgcgatccgc tcgttgatat gccgctagga
    14281 cgcgttcgtg atgaggaaac gtattatgcg aaataccatc aacaccgggt aatgccagaa
    14341 accgaaacgc cgaaggttcg tccagcgtct tcatgcacac ctcaaacgcc gcatcatcta
    14401 ccggttcgta aaccccatta ctgtgtgccc gtgcgatcat ccatgctttt ttcagcggac
    14461 cgcgattgtt gttttttaga attccacgtg tgatcgggcc gaatatgtta atcggattct
    14521 cggtgagatc gaatattgtt ttaatattgg ggttaatatc ccggttcata aaaaacccct
    14581 cccatccgca ataactacgg aagcgatgtg ggttccaaaa aggtttctcg gcaaaggccg
    14641 ttttatcaaa ccagcgaata cccggtaaat tggctttgcc gggaaaacaa cctgtcagaa
    14701 atgggacgta agcagggccg gtggtggacg tgaaaactgt ggtcatgcga cggtaagcgc
    14761 cgcgggctac aatgtgctcc tgaatcgctg gcagacggcc ctcggcaaga aggcgttcaa
    14821 agacatcgta tcgtgcgccg tctatcaaaa tcaaaataat cgaacgaata tatttgatct
    14881 cctataaaat aaaagctgcg ccaaatccga tctgtaggac gaattacaga cggattttgg
    14941 gcagcttaaa cattctttta actaaatcga aatggcaaac acaagcaata aggccatcac
    15001 aatcgctgat cccatagcga ctttttgagt tttactaaaa cgatccgaac ctttactggc
    15061 aagctcaatc gtattgatat tttgtatcgg cacttcgaga ttgcggtact gtttgtctcg
    15121 cgcatcataa tattcgaaca gcacggcctt atcggactcg gcggatttca gataacccgt
    15181 ctcctcaatc aattcttcac ggcttatata aaactgcata cgaacggcaa atttcttatc
    15241 taaaaagcgt tccctcaaca agtccatcgt aatttcttta cgtttataag acgtttgttg
    15301 ggtagcagtg catgcgacaa gatttaatag tatgaataca aatataccgt ttaaaaaaag
    15361 gcgcatgcgt ctcctgtttt atttttcctg cgcttccacc acggcaatag ccgtcatatt
    15421 gacgatatcg ttgacttcgc aaccgcgctg caagacgtgc acgggataac ggaggcccat
    15481 aaggatcggg ccgatggttt ctgcgccgcc cagcttttgg agtaatttat atgctgcgtt
    15541 ggctgattgc aactccggaa atacaagcac gttggcgccg cctttgaggg tactgaacgg
    15601 atagtctttt tcaagtatgg tcgcatccac cgccgtgtcc gcctgcattt cgccgtccac
    15661 catgagtttg ggttcacgtt ggcgaacaat ttcaacggcc ttgcgtactt tttccgcatg
    15721 cggatgacgt gcactgccaa aattactaaa actgatcatg gcgacacgcg gctcggtatt
    15781 aaaccgacgc gccacgcgag cgctgacgat ggcaatctct gccagttctt ccacactcgg
    15841 atcaatattg accgtcgcat cggcgataaa ataaatctga ttttttatga tcagcatata
    15901 caaaccgctg acgcttgata taccttcttc catttcgacg atctggagcg cagggcgaat
    15961 cgtatccgga taatgctgcg acacgcccgc aatgagcgca tcggcggcac gcaagcgcac
    16021 catcatcgaa ccgtacgtcg tatagtttcg tatcgcgttg cgcgcttctg tgagggttgc
    16081 accttttcgc tgacgcatcg cgtaatattt ctctacatac gacaggtaat tatccgacgt
    16141 cgagggatgt atgatctccg caccgctgat gttgacgccg agttcagcag ccgttttttc
    16201 gatcagctcg cgacgcccca aaagtatggg tttggcgata ccttcttcta aaataatatg
    16261 cgccgcccgt aagatttttt catgcgcacc ttccgggaat accacgcgtt taggatgttt
    16321 gcgtgccgta tggatcatgg tacgcatcac ttcacgggat ttgccaagac gccgctctaa
    16381 aagttcctta tactcttcga tattcacttt gatacgcgcc acacccgact ccatcgccgc
    16441 ttgtgcgacg gccgctgctt cccaaatcag cacacggtaa tcaaacggtt tgggaatcac
    16501 atactcccga ccaaatcgaa gccgctcatc gccgtaggct ttagatacgg aatcaggaac
    16561 atcttctttg gctaacttag ccagcgccat cgctgccgcg cgcttcattt cttcattgat
    16621 cgtggttgcg cgtacgtcca atgcgccgcg aaaaatgaac gggaaaccta aaacgttatt
    16681 cacttgatta ggataatcgg aacgaccggt tgcgacgatc gcatcaggcc ttgcggcctt
    16741 agcgtcatca taggtgatct ccggatccgg gttggccatt gcaaaaatga tgggatcacg
    16801 ggccatcgtt ttcaccatgt cttgggtcaa tacattcgcc acggacaatc cggcaaatac
    16861 atcggcgcct ttgagtgcgt ccgccaatgt gcgaagcggc gtatcgttgg ccaaaacttc
    16921 tttgtattta ttcataccct ctttgcgtcc tttatacacg acgcctttgg tatcgcaaag
    16981 aatcagattt tccggtttca cgcccaagct aatataaaaa ttggcgcacg cgattcctga
    17041 agctcccgca ccgttgacga cgacgcgcac ttcatccata cgtttgccta caagttccgt
    17101 cgcattgagc aatgccgctc cggagatgat cgctgtcccg tgttgatcgt catgaaacac
    17161 ggggatgttc atcgtttttt tgagtgtttc ttcgatttca aaacattccg gcgctttgat
    17221 atcttcaaga ttgataccgc caaatgtcgg ctcgagaatc tgacagacac ggatgatttc
    17281 gtccactttt ttagtatcta attcgatatc gaaaacgtca atatcggcaa accgcttgaa
    17341 tagcacacct ttgccttcca tcaccggttt accggctaaa gcgccgatat caccgagccc
    17401 caacaccgct gttccattgg aaatgacggc gactaaattt cctttagccg tgtagttata
    17461 agcgtcttca gggttttttt cgatctctaa acatggcaca gccacgccgg gactgtatgc
    17521 gatggacaga tcacgctggg tcgagcaggg tttggtcggg acaacttcga ttttcccttt
    17581 gcgcccctgg gcatgaaatt cgagaacttc ttctttggaa atcatggtac tcactttggt
    17641 ttacacgaat cagttggcgc tccacggcgg cgtaatacca ttcgcacgcg cgtacgctat
    17701 aagctggcca aggtgttcat gttgatgatt cattgtcgta aaaagtacat tgcgaatggt
    17761 ggcgtctttg cctccgaaaa attttaccgt cttatccatg tcggcatcgt tcatgtttaa
    17821 caccgtgttg cgcaccagtg caaacgaagc tttaatcgtt tccgtggctt ttttggggtc
    17881 ggcatttttt tcaaaattct cgttcatcgg cgtatcggat ttaacgccga taaatccgag
    17941 taaaaaataa tttcctccgg cacaatgcat cagcacttca ccgatggatc gtacgccttc
    18001 agcgggacgg aacccgtatt tatccgcagg aatggtgttt gccatatccg tgtatttctt
    18061 ttccatttga tccaaatgca tgagaaattc ggctcgaaaa ccggaggagg gcgttttttt
    18121 atcctgagcg taaagcgaag cggataaatt caatgcagct aataatagta cgatacgcat
    18181 ggtcagactc cttttgttaa tagtaagacc gcgtaaagta caaggattta gaaataaaac
    18241 aaacatttcg aaaagattaa attacacttt tctgaatgag ccaatatccc ctacaatcac
    18301 agatgaatga atgacaaaaa tcatgttctg tggaattgat tctatgacta tgttagagaa
    18361 tgtagaatgt ggttctggct cagaataacg tatgtggatg acttctattt ggatttagtc
    18421 aaattaagaa acgcagcgga aaaacgatgg ccttcttttt taacttcatc catattgagc
    18481 agtatctggc acttgccctc acggagtttc aaaccaatac tgattttacc ggcgtcatat
    18541 acctccaccg atccggtcac cgtcaaaatc tttttaccct gacaaaaagc cgatacctcg
    18601 ctcgtacgca ctccggcaca tagatacgcg acgtcatatc tttttgaaga ggctaaaaat
    18661 tcttgcgacg tcaaaacatt aacctccgtc atccccgatt tggcaaatgc tgcttttacg
    18721 ttttcgcccg catcatgtga tggcgcgatg atcgctatcg tagggttact actattaggt
    18781 atcgtcagag agtatgcgaa tattttttta taaaattggg attgaacatg caacggcact
    18841 tgaatctcct gcgcatgggt caatgaaata aaaataatga aattgataag ataagaggaa
    18901 actaagacga catgccgcat cataataagc gcctttcctc tggatagatg aattcgaaga
    18961 agtgatgccg taaatatacg gcattggaat ggctttgtaa atacgtaaac tcatgtattc
    19021 aggtataaac cacacaacct ttttcgcgta acgcttcgac tatagagttg ttttctttga
    19081 tttcattcca gcgcaaatcc agtttataga gatgcggtag tcgggttaaa gactccggta
    19141 agtcttttaa atggttttgt cgaaggtcaa gatctgtcaa acgaactaag tcgccaatca
    19201 ttttcggcaa ttcgtataga ctgttattcc gcaaattaag ttctgccaaa ttcttcagct
    19261 tcgccagcgc cgacggtaat ttttttaatt cattattttg taaaaaaagt tttctcaata
    19321 acgataagtt gcccaaacca tccggtaatt tggtcaattg attgttttgc aaatgcagtt
    19381 cgcgaagcgc cgtcaatccg ctccaccgaa tatccatgtt ctcaatacaa ttatcgctcg
    19441 ctcgcaattc gattagcgaa gtaagttccc caattgaagt cggtaaacca ctaaatgaat
    19501 tctctgatat attcagatac cggaggctaa ttagtctttt taatgtttgc gggaaatcgg
    19561 tcaaattgtt atctgtgaga tagagaaatt gcaaagtgct caaatcaccg atttcatcgg
    19621 gtatttcatt gacttcattg tgtccgagat caagcatttc aagacagggg agttcaccga
    19681 tcatata
//
LOCUS       DAOQNQ010000029        19544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_972_length_19544_cov_6.073067, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000029 DAOQNQ010000000
VERSION     DAOQNQ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19544
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_972_length_19544_cov_6.073067"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PK754_03490"
     CDS             <1..443
                     /locus_tag="PK754_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08597.1"
                     /translation="GTCTFDLQGLDAVLKDVTQKYNGEAHIYITGFEAGAHLTWAMTL
                     LHPERLHGTVIVAGNFRNRCLGEAILPVAGEHRNLPLINLVGSLDSSWSSGGRLYDQW
                     LEGKTLAQRQGYVNITEHVIQDKGHRPMPEDVFIQIESLRQKSK"
     gene            complement(610..2046)
                     /locus_tag="PK754_03495"
     CDS             complement(610..2046)
                     /locus_tag="PK754_03495"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNB08598.1"
                     /translation="MSLPLIIQGGMGVAISDWRLARAVSMTGQLGVVSGTGISRIFTS
                     RLMQGDVSGMMREALSHFPSKESVQSVLERYFISGGKSAEEPYKSQPVYSLNPSVFLE
                     QLTTLANYVEVFLAKREHTGIVGINLLEKVQMPNLASLYGAMLAGVDYVLMGAGIPTQ
                     IAGILDKLRNHEPVSYRLDVQNAAPNDDIRFHFDPRKIFPNMAKLTGALKRPKFIPIV
                     SSTVLAQALIKRSEGKIDGFIVETPTAGGHNAPPRGPMRLNERGEPIYGEKDKVDIAK
                     LKELGLPFWLAGGFGHPEKLKEAINNGAAGIQVGTLFSLCDESGMDAQLKKQTLQNVL
                     DKNSQIITNPVISPTGFPFKVTQLKGTVSDPEVYSARERICDLSLLRTVSKNDDGTIV
                     YRCPAEPVEEYVRKGGNVEDTNGRTCLCNNLVAAAGFPQVRKNGYVEPPLVTSGDDLG
                     SVKNFITKDKLHYSAKEVIHYLTEQNNH"
     gene            2478..3416
                     /gene="fmt"
                     /locus_tag="PK754_03500"
     CDS             2478..3416
                     /gene="fmt"
                     /locus_tag="PK754_03500"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNB08599.1"
                     /translation="MKKLRIVFMGTAAFAVPSLRRLAQSHHEVVAVVTTPDLPRGRGQ
                     QVSFTPIKELAVELGLPVLQPDNLKDEQFIASLRSYNADVFLVVAYRILPPEVFELPS
                     LGCINLHASLLPKYRGAAPINWAIMRGETETGVTTFFIQKKVDTGTIILQRKTIIGEN
                     ETVGELHDRLSEIGADVFLETADLIAENKVVTRVQDDQLATTAPKLFKETCRIDWTRS
                     SREIKNTVRGLSPYPCAWTTIDGEAYKIFRIETVKTAEQSAPGTIIVSNKQDGIWIQT
                     GDGVVAILELQPPSKKRMKVTDFLNGRQLPIGICAG"
     gene            3514..4542
                     /locus_tag="PK754_03505"
     CDS             3514..4542
                     /locus_tag="PK754_03505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB08600.1"
                     /translation="MLFIESFVLGKNNQFQPVREGRVTYATVPFFKATPYPLINKLQV
                     LWPFRTVIEWIASLWVFWLCRKTGFDAPERVIATSNIYYADIIRRMKKKAVIYDCNDY
                     PLGFALLLPTAKEFFKKTIRLSNVTTTVSKKLVEDIIPHNPSRVSVIGNGVDFKLFDS
                     KPAIKPVEMPTDRPVVFFSGAVAEWFNVELLTRLIESLPEAAFVVLGPIKHAVTGEQL
                     RILAQVYGLILISEKKHAELPSYMHSASVCILPFRNNRRIWGANPNTLYEFLACGKPT
                     VTFGFSEEVRNLAPHVYIADSDEDFVQYVGKALRSKADTEKLKSLAAKNSWSIKAAQM
                     TDEIRRVV"
     gene            4539..6095
                     /locus_tag="PK754_03510"
     CDS             4539..6095
                     /locus_tag="PK754_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08601.1"
                     /translation="MKKTYLYIFLFLFSVNLLFTGGRIASSDETAYFLEAQSVVERGT
                     LAIPTSIVNNGAFGVDGRFYVGGGVGYTILGAPMYAFGLLITKALPIPETYSLFVLKG
                     IFTLINPILCALLGLLMIHYLRFMSVSWGFAFLWASALLFTTNVFVYSKSSMREPLIT
                     VCVLMAIMEIFTFGRTSLRKHLHLAGIAAGWLVLIKPSFLIFVPVFGLYLVWVLYDNR
                     LHVKDWLRAANVVIPVSIFSAWVSVFIGMAVCYNWLIFGGPFTSGYSKIQNPFSNPFL
                     VGVYGLLFSSGKSLFLYAPLTILIFPAARCFLQREFKLFTAITVVCCTFIALHAKFVA
                     WAGDGSWGPRYLLPIVPLLFIVIGTYAYETIKKNRTLRFMIQTLAVIGLLIQFGGMTI
                     YLGSYYRYLGEYPYTREFSDPEFLYKSHFVPNYSPVVGHWELMGKAISKHWNGDIGVL
                     SIRATDERIPLSDEDRSKVTYLIDYWFMYLYYAGVSKILILLSVIVMVGFVIVAGWNV
                     RRQLQKVEIQ"
     gene            6092..6811
                     /locus_tag="PK754_03515"
     CDS             6092..6811
                     /locus_tag="PK754_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014649286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB08602.1"
                     /translation="MNRLLVIIPAYNEASNITNVVLRVQKATDTLSNIRATVLVVNDG
                     SKDNTSDCARATGAVVLDLPYNLGIGGAVQCGFRYARDKKFDMAIQIDGDGQHDPSFI
                     HKLLSPLQEGAADMVIGSRFLNREGFQSTWLRRVGIRIFEGVNSAIIGQRITDNTSGF
                     RAYNKRAIALLAEEYPSDYPEPEAVIVLGLRGFKICETPVVMRERQGGISSIGSMSSV
                     YYMAKVLLAIGVNVFKKQNQR"
     gene            6834..7229
                     /locus_tag="PK754_03520"
     CDS             6834..7229
                     /locus_tag="PK754_03520"
                     /inference="COORDINATES: protein motif:HMM:NF021575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HNB08603.1"
                     /translation="MPELNTGVEIFRIQIIAVAGSLFIIGFIIELIRRKKLQERYALL
                     WLVAGIVMIMLSVWRDGLDKLAHVLGVGYAPALLFLVALGFGLLMMIHFSVVISSLTE
                     RNKILAQEIALLKAELNKINDESKNESGN"
     gene            7239..7856
                     /locus_tag="PK754_03525"
     CDS             7239..7856
                     /locus_tag="PK754_03525"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB08604.1"
                     /translation="MSKNLQTGTVNTPDFWDHIYQNERPRWDIGHATPVFAEWLAHRN
                     QLGTGSIAVLGCGTGHDAALFSQHGFDVTAVDFAESPLQSLQKNYPGIRTLQKDIFEL
                     NESHNEVFDFVLEYTCFCAIDPMRRDEYVQIVHTILKPGGYLIGLFFPLDERPDGPPF
                     AVRYDDVDRHFSSTFISLLDEIPEHSVPKRREKERFVIYQKKHQS"
     gene            7904..8686
                     /locus_tag="PK754_03530"
     CDS             7904..8686
                     /locus_tag="PK754_03530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HNB08605.1"
                     /translation="MANAKDNLKRYTRLRNLTATLETFIFGGYRMLAKHNIDAASHGV
                     SISECTYSPWKINNEFNDLYEKIKNYTLVDKRKLYELWSLSRQLKFVRGHYIEIGVWR
                     GGSGVLLGKTLMDHGAGEKIYLCDTYEGMPAITDQNDNFYKGGELADTSTEMVHQLAH
                     DVGLNNVEILKGIFPRDTASMIPSNEVFKLVHIDVDIYSSAKETMAWVWGRMPSGGMV
                     IFDDYGYSATEGVTRFVHEFMNERNDALFIFNLLGHGIVIKR"
     gene            8753..10273
                     /gene="lysS"
                     /locus_tag="PK754_03535"
     CDS             8753..10273
                     /gene="lysS"
                     /locus_tag="PK754_03535"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNB08606.1"
                     /translation="MEEELLRVRREKLDKLREAGINPYPYKYERDAMSQELTENFEAL
                     GVKDDTQLDNPDVRRIRVCGRIMSLRSKGKTAFAHIMDPQGKIQIYVRKDAIGEAAYE
                     AFSKLYDIGDHIGVKGVLFKTHSGEITIKAEEVVLLSKSMRPLPAGKEKINDDGTVQR
                     FSAMDDVELRYRQRYADLILRPEVRDTFEKRSRVISGIREFLDKHGYLEVETPILQPL
                     YGGASARPFVTHHNALDIKLYMRIALELYLKRLIVGGIDRVYEISKIFRNEGMDRTHN
                     PEFTMLEFYAAYQDFNDLMTFTEDMLGFVCHRVHGKDSIESMGHTVSFKPPYERLSMF
                     DAIKKYTQIDVSAMDEAALREVCKTLHIPTEKSMDRGKLIDEIFSAKVQEHLIQPVFI
                     TEQPIEISPLAKKHRSKNGVVERFELFILGSEVANAFSELNDPLDQRERFEAQMGLRA
                     RGDEEAQVLDEDFLRALEYGMPPTAGEGIGIDRLVMIMTGQDSIRDVLFFPTMRPE"
     gene            10277..11092
                     /locus_tag="PK754_03540"
     CDS             10277..11092
                     /locus_tag="PK754_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB08607.1"
                     /translation="MFGYIRNIILTIGSYTLLDKLGKKTISFFTGLGESAILVFQAAR
                     NLRYVFKDRHLIVEQMNLVGVNSLPLVCVTAIFAGMTSAVQAGYQLQGVASPNLLLGS
                     ATSRAITTELGPVLTGLVLSGRFAASIAAEIGTMKVTEQIDALEMLAINPIRYLALPR
                     ILAGLLMMPVLIIFTDIVAICGSMFIGFTTMDVSPQAFLSSMQKFMTMTDLFSGLIKA
                     VFFGASTAIVGCFVGFATEGGAEGVGMATIKAFVLSSAAILVNDYLVASVLLL"
     gene            11108..11869
                     /locus_tag="PK754_03545"
     CDS             11108..11869
                     /locus_tag="PK754_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB08608.1"
                     /translation="MIQVFDLYKYFRDKKILSGVNLEIESGKVTTIIGRSGCGKSVLL
                     KHIVGLLTPDRGRVLIENEEIGKLTRDELSEVRKKMGLVFQNGALFDSMTVEENITLG
                     LYEHTKMSKQEMSVKAAKLLDMVGLPGIEKYKPSELSGGMRKRVSLARALAMDPKYVL
                     YDEPTTGLDPIMSAIIDDLIVRLNEELKITSIVVTHDMRSVYTISDRVAMLHYGQVRF
                     NGTIDQLKSTNDPVVQQFIEGRIEGPIQPVLKDGI"
     gene            complement(11870..12613)
                     /locus_tag="PK754_03550"
     CDS             complement(11870..12613)
                     /locus_tag="PK754_03550"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNB08609.1"
                     /translation="MRVSLKFLLGLFIVANTTQSQTRNVSYSLPYDTARGGLSINYDW
                     MQEIEPDVATITLSVDAQRSDLRSASEQNKLLADKILDALKNYPCSLRFDPAHTLSVP
                     STNAYSGYSNSITEPDYVVSRTAVIMTPIITSYPDIMRALSQINGMTINRIDFSTSKM
                     DSLGAEALKNAQTVLRKKAEEAGKTLGFKPGKLYSFSYAAPQLEPEYAVLSYPISSPS
                     QIQPMSNQMPHPGKLLFKITLSGMFEITP"
     gene            12711..13829
                     /gene="apbC"
                     /locus_tag="PK754_03555"
     CDS             12711..13829
                     /gene="apbC"
                     /locus_tag="PK754_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HNB08610.1"
                     /translation="MITEELIFQALKSVQDPDLHKDIVSLGFVKDIVIDGGKVAFKIE
                     LTTPACPVKDLLKQQSHDAVAKISGVTSVEIIMTSRIPANKGSMNQSNLLPGVKNVIA
                     VASGKGGVGKSTVATNLAVALGMLGAKVGLLDADIYGPSIPLMMGTSEKPGSTDGQRM
                     DPILHHGIQMMSIGFLMPEEQALVWRGPMVAQALTQLMRDVAWGELDYLIVDMPPGTG
                     DAQLTLSQVVHLVGAVIVTTPQDVALLDAKRGVGMFQKVNVPILGIIENMSYYVCPNC
                     NHRTDIFSHGGAQKAAEKYEVPFLGEVPLDIETRMAGDAGTPIVLAKPDSVNAKAFMQ
                     VARNLASMVSIIHYGTAEDRSKALGQKTFFANTRLKVI"
     gene            13914..13996
                     /locus_tag="PK754_03560"
     tRNA            13914..13996
                     /locus_tag="PK754_03560"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13947..13949,aa:Ser,seq:tga)
     gene            14034..14124
                     /locus_tag="PK754_03565"
     tRNA            14034..14124
                     /locus_tag="PK754_03565"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14068..14070,aa:Ser,seq:gct)
     gene            14453..14734
                     /locus_tag="PK754_03570"
     CDS             14453..14734
                     /locus_tag="PK754_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08611.1"
                     /translation="MSNLFFDSNYIIIEYPLQKKKIQILKKDIVEITTIKNTERDMKK
                     DGGVSIKTKTNEYRIYWMFHKEIKLIKRKLEEIRPIQPYRGIWSEILKK"
     gene            14731..15138
                     /locus_tag="PK754_03575"
     CDS             14731..15138
                     /locus_tag="PK754_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08612.1"
                     /translation="MIKIASFLITIGLFFVSLVIFGIYVVGTHSMDYNIEALYGVFII
                     MNFMMTVFGLNTFLFKTLTSLQAEKLTLLLKFTLPISVFSFIWYIVDSINGALESDII
                     YIGNIEILFWLLLLAALIFLTLKIFNWKEKNEQ"
     gene            15135..15851
                     /locus_tag="PK754_03580"
     CDS             15135..15851
                     /locus_tag="PK754_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08613.1"
                     /translation="MKYTFLFLLIIQQSDQCVGQTSKEDVARLYVAYFELYIYSSDII
                     LGNNTYKSSKNSLATSIVSPKEIKEKYVSWKYKEKFNGLEVFCVSHACRYCSPYIVGI
                     DDELNIYRMYGFGTNISDISEQIDRNYNDTTYYLDFRDQKSILSVDVKSFVKNYLRLV
                     EFGDISENLEFTSEEVDLISTTILGRITTKYYVSRRDNKFVEIDWKYAISRDGYVSLS
                     QNASRNHGVEIIDGKVIDEN"
     gene            16124..17140
                     /locus_tag="PK754_03585"
     CDS             16124..17140
                     /locus_tag="PK754_03585"
                     /inference="COORDINATES: protein motif:HMM:NF024704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HNB08614.1"
                     /translation="MIRKSFICYSLVFITTVMSYNSMGSPSKNPIFIDITGGVFPLEN
                     TITESLDSNVKIVINTKFYLRVFYSDYVLFMEKSSKKRMGNRKLRDYELHRVNLLSKT
                     DSIFSIRTLHDELSAYKTIKNYCYALHGKVFRSDSIQSQLNTEGWYVSNQKYSDQLLD
                     EIDWIEIDHAKNQVKKINDSFASFKNILKKFDNTLAPLPRIYVLKLDSSNFAVSNMRI
                     WLKEDSLPALFKKPQIFNLIYDDHFGDKPAKQYYVDGLDVICYHDKVIGVTTVNPKFA
                     TQNNIHVGMSLKELLKKASPVDEEKIPSNCVIRLSCTNEWGYLSYFFILLINEKISMM
                     LLES"
     gene            17629..18141
                     /locus_tag="PK754_03590"
     CDS             17629..18141
                     /locus_tag="PK754_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB08615.1"
                     /translation="MPIRSTLFRMMILAISSLSVGIAFNQWHSSGFAWDKLIRRDHSK
                     TYMRMPRQDASKLQRLSLDEAYTLFKTKAGLFVDARQEAIFKLAHIQGAVSCYYSRAK
                     ELPIVNEWEKNKTLITYCGGPRCDQADRLAMQLIEMGFTNVFVFTGGMEEWRAAGYPI
                     EEQKRSTHPE"
     gene            18152..18619
                     /locus_tag="PK754_03595"
     CDS             18152..18619
                     /locus_tag="PK754_03595"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HNB08616.1"
                     /translation="MAWKSHIKTALRLILGIVFCYGALDKITSPYAFAAAIDAYRILP
                     AVVTPISAVWLAWLELFIGIALMVGYWTRAAAGWAILLLSIFIFAITSALLRDLVIPC
                     GCFDVNDPDEVISWKKIAEDGVLLMIAVYVFISPYARWSLSKEERLSYDGKLS"
     gene            complement(18593..>19544)
                     /locus_tag="PK754_03600"
     CDS             complement(18593..>19544)
                     /locus_tag="PK754_03600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB08617.1"
                     /translation="YYYRIQARYGNASSDVSGLFFVRPNPKPSVVSVEMYNANFIRAR
                     FSEPIHNESLSQNFHYLLDDRYYPASAIPVEGGQAVMLHFTQLASGTHRLFFYSQHDT
                     DGTPSDTATRTFLYDGVTQRTPLYIVQATYEGRNRIRLIFNKSVDIATATRTSTYRMN
                     YDFSIVSASIVPLSSNTEVFLDVDGKKYLGPIGLAFTIDAHGVKDSEGYPIDSVFGYR
                     AGLLFSVEKLDDPFVYPNPHRADIDPPYITFAGIPDKTVVRIYSVDGRFIKQIAESGN
                     DGGVRWDLKDKNGKRISSGVYIYEAVVSGKKKLGKFAVVR"
BASE COUNT         5466 a         3794 c         4575 g         5709 t
ORIGIN      
        1 aaggaacttg cacttttgac ttgcaaggtc tcgatgccgt attgaaagat gtaactcaaa
       61 aatacaacgg tgaagcgcac atatatatta caggttttga agccggagcg catttaacat
      121 gggcgatgac actattgcat ccggaaagac tacatggcac agtcattgta gccggtaatt
      181 tcagaaaccg ttgcctgggt gaagcgatac ttccggttgc aggggaacat cgtaatttgc
      241 cactgatcaa cctcgtcggc agtttggatt cttcctggtc atccggcgga cgcctctacg
      301 atcagtggct tgaaggtaaa actttagcac aacgtcaggg ttatgtcaat ataaccgaac
      361 acgtaataca ggataaaggt cataggccga tgcccgaaga tgtttttatt cagatcgagt
      421 cacttcgaca aaagtcaaag tgacttatga tttcgcacaa atttgcattt tcattagtat
      481 cattagtttt gtgaagaaca caaaccattt ttaaatcaaa aaatcgttac gactttgcat
      541 aaaaaaacga ggttgctgaa cctcgttttt tgttgatact cttaatagag tcagtatttc
      601 tacattcgat taatgattgt tttgttctgt aaggtaatgg ataacttctt tggcggaata
      661 gtgtaattta tcttttgtga tgaagttttt cacacttcct aaatcatctc cagatgtcac
      721 cagaggcggt tcgacatagc cgttttttcg aacttgcgga aatccggcag cggccaccag
      781 attattgcag agacaagtgc ggccattcgt atcttcgaca ttgcctcctt tgcgtacata
      841 ttcttccacc ggctcggcgg gacagcgata gacgatagtt ccgtcgtcgt ttttggagac
      901 cgttcgcaaa aggctcaagt cgcagatacg ctcgcgagcg gaatatactt caggatctga
      961 cactgtacct ttgagttgtg tgactttgaa aggaaacccg gtaggggaaa taacgggatt
     1021 agtaattatt tgtgaatttt tatcaagcac attttgcaaa gtttgttttt taagttgcgc
     1081 atccattccg gattcatcgc ataatgaaaa taaagtgccg acttgaattc ctgcggcgcc
     1141 attatttata gcttctttca gtttttccgg atgcccgaaa cctccggcta accaaaaagg
     1201 cagacctaat tcttttagct tcgcaatatc tactttatct ttttctccgt atatcggctc
     1261 tccccgttca tttaatcgca tcggtccgcg cggtggtgca ttatggcctc cggccgtcgg
     1321 cgtttcgacg ataaagccat cgattttacc ttcgcttcgt ttgatcaaag cttgcgcgag
     1381 tactgtggat gaaacgatcg gaataaattt gggacgtttg agggcgcctg tcaattttgc
     1441 catgttggga aaaattttac gcggatcaaa atgaaaacga atatcatcgt tgggagcggc
     1501 attttgaacg tctaagcgat aactgacagg ttcgtgattt ctgagtttgt ccaatattcc
     1561 tgcaatttga gtcggaattc ctgctcccat caatacatag tcaacgcctg ccagcatagc
     1621 tccatagagc gaagcgaggt taggcatttg cactttttca agaaggttga ttccgacaat
     1681 gcctgtatgt tcacgtttag caaggaaaac ttctacataa ttggctaatg ttgttagttg
     1741 ctccaaaaaa acagacggat ttaacgagta aaccggctgt gatttataag gttcctctgc
     1801 ggattttccc ccagagatga aatagcgttc gagaacggat tgtacggatt ccttagaggg
     1861 aaaatgagac agcgcttcgc gcatcatacc cgacacatcg ccctgcataa gccgtgaagt
     1921 gaatatacgg ctgatcccgg tgccggacac aacacctaac tgaccggtca tagaaacagc
     1981 tctggccaac cgccagtcgg aaatggcaac acccatccca ccttggataa ttaaaggtaa
     2041 actcatgata actccgttat tgataagacg attacatatt atatggaatt ataatagtag
     2101 ctcatattgt caggcattcc tatgatcacc gtcagtactc aaataatcag aaatcacaaa
     2161 cagtcgctac acaatcaata taggttatag atacaaacaa aggcgaaatc gctgtagaag
     2221 cctacagaga aggcttttgt cgtcaattgt gacaatgttc aaattggttt atggtagttt
     2281 cgttaacaga ttcgcctgat aaaaatcaca ttatcagagt ataatctgat gtttcaaatt
     2341 ttgatttatt cgcacataat gacatgattg gcttgattac tgcttgtcat acgcaatact
     2401 attctaacga agataatctg cgcactctgt aaaatctgct tcatttttcc cgtaaaacat
     2461 gctacctttg ccgcgctatg aaaaaactgc gcattgtatt tatgggaact gcggcatttg
     2521 ccgttccttc gctccgccga ctagcccaaa gtcatcacga agtcgtagct gttgttacga
     2581 cgccagatct tcctcgcggt cgcggtcagc aagtgagttt tacgcccatc aaagaattgg
     2641 ccgtcgaact gggtctgcct gtacttcaac ctgacaatct caaagatgag caattcattg
     2701 cgtctttacg ttcttataat gcggatgtgt ttttagtcgt agcgtatcga atattaccgc
     2761 cggaagtttt tgaattgccg tccttggggt gcatcaattt gcacgcttct ttactgccca
     2821 aataccgcgg tgcggcacca atcaattggg cgatcatgcg cggtgaaacc gaaaccggag
     2881 tcactacttt ttttattcag aaaaaagtgg ataccggtac gattattctt caacgcaaaa
     2941 ccatcattgg tgaaaatgaa acggtgggcg aattgcatga tcgtttatcc gaaataggcg
     3001 cggacgtgtt tttagaaacc gctgacctta tcgcggagaa taaggtggtg acgcgcgtgc
     3061 aggatgatca attggcgaca acggcaccta aactattcaa agaaacatgc cgtattgact
     3121 ggaccagatc gtcacgagaa ataaaaaata cggtgcgggg actatctccc tatccgtgcg
     3181 catggacgac cattgatggt gaagcgtaca aaatatttcg tattgaaacg gtcaaaacag
     3241 ccgagcagtc ggctcccggt acgatcatag tctctaataa acaagacgga atatggattc
     3301 aaaccggtga tggagtggtc gcgattttgg aattgcagcc tccgtccaaa aaacgaatga
     3361 aagtaactga ttttttaaat ggccgacagt tgccaatagg tatttgtgca ggatgatgat
     3421 gctatgaaag ttgtttggtt ttcagaaata aaatggacgt atctcaaaac gcgtaagcag
     3481 cagatcattg aaaattttcc accggaatgg gaagtgctat ttatcgaaag ttttgtgtta
     3541 ggaaaaaata atcaatttca acctgttcgt gaggggcggg tcacttatgc caccgtgcct
     3601 ttttttaaag ctacgcctta ccctctgatt aacaagttgc aagtgctttg gccatttcgt
     3661 acggttatcg aatggatagc gtcattgtgg gtgttttggt tatgtcgaaa aacaggattt
     3721 gatgcgccgg aaagggtcat tgcaacatcc aatatttact atgccgatat cattcgacgc
     3781 atgaaaaaaa aggctgtgat ttacgattgc aatgattatc cgttggggtt tgctctatta
     3841 ctcccaaccg caaaggaatt ttttaaaaaa accattcgac tttcgaatgt tactacgacg
     3901 gtttccaaaa aattagtaga agacatcata cctcataatc catcgcgtgt gtcggttatc
     3961 ggaaacggtg tagattttaa attatttgat tcgaagcctg ctattaaacc ggtggaaatg
     4021 cccacagacc gacctgtagt attttttagc ggagctgtag cagaatggtt caatgttgaa
     4081 ttattaacac gactgatcga atcactccct gaggcggcat ttgtcgtatt aggcccgatc
     4141 aaacatgcgg ttaccggtga gcagctacgg atactggctc aagtttacgg gttgatattg
     4201 atctcagaaa aaaaacatgc tgaactacct tcgtatatgc acagtgcaag cgtgtgtatc
     4261 ttaccattcc gcaataaccg tcgtatttgg ggggcaaacc caaataccct ctatgaattt
     4321 ctagcctgcg gtaaaccaac tgtaacgttt ggcttctctg aagaagttcg taatttagct
     4381 ccacatgttt atattgcgga ttccgacgaa gactttgttc aatatgttgg caaagctcta
     4441 cgtagtaagg cagatacaga aaagcttaaa tctttagctg cgaaaaacag ttggtcaatc
     4501 aaagcagcgc aaatgacaga cgaaattaga cgcgtcgtat gaaaaaaacg tatctatata
     4561 ttttcttgtt tttattttct gtgaatttac tcttcacggg cgggaggatt gcatccagcg
     4621 atgagacggc atattttctc gaagcgcaaa gtgtagtcga gaggggaacg ctggccatac
     4681 cgaccagtat cgtcaataac ggcgcattcg gcgtggatgg ccgattttat gtcggaggtg
     4741 gtgtcggtta tacgatactg ggtgcaccga tgtatgcctt cggcttattg ataaccaaag
     4801 cacttccaat tcctgaaacg tattcgcttt ttgttctcaa gggcattttc acgctgatca
     4861 atccgatctt atgtgctctg ctcggccttt tgatgataca ttacctacgt ttcatgagcg
     4921 tatcctgggg tttcgctttc ttatgggcat cggcgttgtt gtttacaact aatgtgtttg
     4981 tgtattctaa atcttcgatg cgcgaacccc tgataacagt atgcgtactg atggccataa
     5041 tggaaatttt tacatttggt cgaacttcat tgcgaaagca tctgcatttg gccggaattg
     5101 ctgcaggttg gcttgtattg atcaaacctt catttttgat ttttgtaccg gtttttggtt
     5161 tatatctagt atgggtttta tatgataacc gtcttcatgt taaggattgg ttgcgagcgg
     5221 caaatgtggt tataccggta agtatcttca gtgcctgggt gagtgttttt atcggaatgg
     5281 ccgtatgcta caattggtta attttcggtg ggccttttac atcgggttat tccaaaatac
     5341 aaaacccttt ttcaaatcct tttttagtcg gggtatacgg attacttttt agttccggaa
     5401 aaagtttatt tctctacgca ccgcttacca tacttatatt cccggcggca cgttgttttt
     5461 tacaaagaga gttcaaatta tttacagcaa taacggtagt gtgctgcaca ttcatagcgc
     5521 tccacgctaa atttgtagcg tgggccggtg acgggagttg ggggccgcga tatcttcttc
     5581 cgatagttcc tttgcttttt atagtcatag gaacttacgc atatgaaact attaaaaaaa
     5641 accgaacttt gcgttttatg atacaaacgc ttgcggtgat cggtctgctg attcaatttg
     5701 gtggaatgac catctacctg ggaagttact accgatattt gggcgaatat ccttatacgc
     5761 gggaattttc tgatccggaa tttttgtata aatcgcattt tgttcctaat tattccccag
     5821 tggtcggtca ttgggaactc atgggcaagg ctatcagtaa acactggaac ggggatattg
     5881 gtgtgttgtc cattcgcgcc acggatgagc gcattccgtt atcggatgag gatcgttcca
     5941 aagtaacgta tctcattgac tattggttca tgtatctcta ttatgccgga gtttcgaaaa
     6001 tcttaattct attgtctgtg atagtaatgg ttgggttcgt catagtagcg ggttggaatg
     6061 ttcggcgaca attacaaaaa gtggaaatcc agtgaatcgt cttttggtga ttattcctgc
     6121 atataatgaa gcttcgaaca taacgaatgt cgtgcttcga gttcaaaaag ctacggatac
     6181 gttgtcgaat attcgtgcga cagtgctcgt agttaatgac ggatcaaagg acaacacttc
     6241 cgattgtgct cgtgcaaccg gcgcggttgt tttggatttg ccatataatc taggtattgg
     6301 cggtgcagta caatgcgggt ttcgatatgc acgcgacaaa aaatttgata tggctataca
     6361 gattgacggg gacggtcaac atgacccgtc ctttattcat aaattgttaa gccctttgca
     6421 agaaggtgcg gctgatatgg tgatcggctc acgttttttg aaccgagaag gattccagtc
     6481 cacatggctt cgtcgtgtcg ggattcgtat ttttgaggga gttaattcgg caattatcgg
     6541 tcaacgtatc acagataata catccggttt tcgcgcgtat aataaacggg ctatcgcgtt
     6601 gcttgccgag gaatatccat ccgattatcc ggaaccggaa gccgtcatcg tattgggttt
     6661 gcgcggattt aaaatttgcg aaacgccggt cgtcatgcgt gaacggcaag gtggtatatc
     6721 ttcaataggt tcgatgtctt cggtttatta tatggctaaa gtattactgg ctataggtgt
     6781 caacgttttt aaaaaacaaa accaacgtta ggtgcgaatc acgaggtata gatatgcctg
     6841 aattaaatac cggggttgaa atttttagaa ttcagattat cgcagtggcg ggtagtcttt
     6901 ttatcattgg ttttataata gaattgatac gtagaaaaaa gctgcaagaa aggtacgcgc
     6961 ttctgtggtt agtcgccggg atagttatga tcatgttgtc ggtatggcgc gatgggttgg
     7021 ataaattggc ccacgttttg ggtgttggat acgctccggc gttattattc ttagtggcgc
     7081 ttggtttcgg gttacttatg atgattcatt tttcggtagt tatttcttcg ctaactgaac
     7141 gcaataaaat actcgcgcaa gagattgctt tgttgaaggc tgagttaaac aaaatcaatg
     7201 atgagtctaa aaatgagagt ggaaattaat ctaatctgat gtcaaagaat ttacagactg
     7261 gtacggtcaa tacccctgat ttttgggatc atatctatca aaatgagcgc ccgcgttggg
     7321 atatcgggca tgcaacgccg gtgtttgcgg aatggcttgc gcatcgcaat caacttggca
     7381 cgggatccat cgcggtactg ggttgcggta cagggcatga tgcggcgctt ttttcgcagc
     7441 atggttttga tgtgacggca gttgattttg ccgaatcgcc gttgcaatcg cttcagaaaa
     7501 attatcccgg cattcgtaca ttgcaaaaag atatttttga gttaaatgag tcgcacaacg
     7561 aagtgtttga ttttgttttg gaatacacat gcttctgtgc gattgatccg atgcgacggg
     7621 acgaatacgt gcagattgtc catacgattc tcaaaccggg agggtacctg atcggtttgt
     7681 tttttccgtt ggacgaaaga ccggacgggc caccgtttgc cgtacggtat gacgatgtgg
     7741 acaggcattt ttcatcgacg tttatatcat tgctggatga aataccggag cactcggttc
     7801 ccaagcgaag ggaaaaagaa cgatttgtaa tttatcaaaa aaagcatcaa agttagaaga
     7861 attaatattt aacttcctca gtccaaaaag gagtataaaa gaaatggcca acgcgaagga
     7921 taacttaaaa cgttatacac gtctgcggaa tttgactgca acattagaaa cttttatttt
     7981 cggtggttac cgtatgctcg caaagcataa tattgatgcg gcttcccacg gtgtaagtat
     8041 ttccgagtgc acttatagcc cttggaaaat caataatgaa tttaatgatc tttacgaaaa
     8101 aataaaaaat tatactttgg tagataaaag aaaactttat gaactttgga gtttgtctcg
     8161 tcaattaaag ttcgttcggg gacactatat tgaaatcggt gtgtggcgag gggggtcggg
     8221 cgtactcttg ggaaaaacac ttatggatca tggagcgggc gagaaaatct atttatgcga
     8281 cacatatgaa ggaatgccgg ccattacgga tcaaaatgat aacttttaca aaggaggaga
     8341 attggccgat acctcgactg aaatggttca tcaattggcg cacgatgtag gtcttaacaa
     8401 tgttgaaatt ttgaagggca tctttccccg tgatacagca tctatgattc cgtcgaatga
     8461 agtattcaaa ttggtacata ttgatgttga tatctattcg tccgctaagg aaacgatggc
     8521 atgggtatgg ggacgaatgc cgtctggtgg gatggtgatt tttgatgact atgggtattc
     8581 cgcgactgaa ggcgtaacgc ggtttgttca cgaatttatg aatgaaagaa atgatgcatt
     8641 gtttatattt aaccttttag gtcatggcat tgtaattaag cgttgaggtg aagaggtacc
     8701 atttaatact ataggtacca tttaatacta tagataaagg taagagggca ttgtggaaga
     8761 agaattatta cgtgtacgac gggaaaaatt agacaagctt cgtgaagccg gtatcaatcc
     8821 gtatccttac aaatatgaac gggacgcgat gtcgcaggag ttgacggaaa actttgaagc
     8881 gctcggcgtc aaagatgaca cgcaattaga caatccggat gtacgtcgga tacgcgtatg
     8941 cggtcgtatc atgtcgttgc gcagcaaagg caaaacagcg tttgcgcata ttatggaccc
     9001 gcaggggaaa attcagatct atgtacgtaa agacgctatc ggtgaagccg catatgaagc
     9061 gtttagtaaa ctgtatgata tcggcgatca tatcggtgtc aaaggcgtac tttttaagac
     9121 acactccggc gagatcacca tcaaagcgga agaagtcgta ttgctttcca agagcatgcg
     9181 tccgcttccg gccggaaaag aaaagatcaa cgacgacgga acggtacagc gtttttccgc
     9241 gatggatgat gtggagttga gataccgcca acgttatgcc gatctgatcc tgagacccga
     9301 agtgcgggat acttttgaaa aaagatcgcg cgtgatttcg ggtattcgcg aatttttgga
     9361 taaacacggt tatctggaag tcgaaacgcc gattttacag ccgttgtatg gcggtgcatc
     9421 cgccaggcct tttgtgacgc accacaatgc gctcgacatc aagttgtata tgcgtattgc
     9481 gttggagctg tacctcaaac gactcatcgt cggaggtatt gaccgcgtgt acgaaatttc
     9541 caagatcttt cgtaatgaag gtatggatcg cacgcataat cccgaattta cgatgctcga
     9601 gttttatgct gcgtatcagg attttaatga tctcatgaca tttaccgaag acatgcttgg
     9661 ttttgtctgc catcgggtac atggtaaaga cagcatcgaa tccatggggc atacggtatc
     9721 gttcaaaccg ccttacgagc ggctctctat gtttgatgcg atcaaaaaat atacacagat
     9781 cgatgtatcg gctatggatg aagccgcgtt gcgtgaagtt tgcaaaacgc tgcatattcc
     9841 taccgaaaaa tccatggatc ggggaaaatt gattgacgaa atttttagcg caaaagtgca
     9901 ggagcatctg atccaaccgg tgtttatcac cgaacagccg attgagattt ctccactggc
     9961 caaaaaacac cgttccaaaa atggcgtggt cgagcggttt gaattattta tcctcggcag
    10021 tgaagtggcc aacgccttca gcgagctgaa tgatccactg gatcagcgtg aacgcttcga
    10081 agcgcagatg ggtttgcgtg cgcggggcga cgaagaagcg caggttttgg atgaagattt
    10141 tctgcgggct ttggaatacg gcatgccgcc gacagcgggg gaaggtatcg gtatagatcg
    10201 ccttgtcatg atcatgacgg gtcaggattc catacgcgat gtgttatttt ttcctacgat
    10261 gcgaccggaa taaactatgt ttggatatat tcgaaatatt attctcacga tcggcagtta
    10321 tacgctgctc gacaaacttg gaaaaaagac catttctttt ttcacgggtt tgggcgaatc
    10381 ggccattctc gtttttcagg ctgcacgcaa tttgcggtat gttttcaaag atcgtcattt
    10441 gatcgtcgag cagatgaatc tggtcggcgt taattcactg ccgctggtat gtgttacggc
    10501 gatttttgca ggtatgacat ctgccgtcca agccggttat cagcttcagg gtgtagcttc
    10561 acctaatctg ttgctgggtt cagcaacgtc acgtgcgatc acgacggagc ttggaccggt
    10621 gcttaccggt ttggttttgt cggggcgatt tgcggcatcc atcgctgcgg agatcggtac
    10681 gatgaaggtg acggagcaaa tagatgcgct ggagatgctt gcaatcaatc cgattcgtta
    10741 tctggcattg cctcgtattt tggcggggct tctgatgatg ccggttctga ttatctttac
    10801 ggacattgtt gcgatttgcg gttcgatgtt tatcggattt acgacgatgg atgtttcgcc
    10861 gcaggcattt ttgtccagca tgcaaaaatt tatgaccatg accgatttgt tttccggtct
    10921 tatcaaagcc gtattttttg gcgcctctac agcgatcgtc ggatgttttg tcggttttgc
    10981 cacggaaggc ggagccgaag gtgtggggat ggccacgatc aaagcgtttg tattatccag
    11041 cgcggcgatt ctcgtcaatg attatttggt cgcctccgta ttgctcttgt aattagaaaa
    11101 aacaactatg atccaggttt ttgatttata caaatatttc cgcgataaaa aaattctcag
    11161 cggcgtcaac ctcgaaatcg agtccggtaa agttacgacc attatcgggc gcagcggttg
    11221 cggtaaaagt gttttactca aacacatcgt cggcttattg acgccggacc gcggacgcgt
    11281 tcttatcgaa aacgaagaaa taggaaagct gacgcgtgac gaactttccg aagtgcgaaa
    11341 aaaaatgggg ctcgtgtttc aaaacggcgc tttatttgat tcgatgaccg tcgaagaaaa
    11401 tatcacactc ggcctgtatg agcataccaa aatgagcaaa caggagatga gtgtcaaagc
    11461 cgccaaattg ctggatatgg tcggcttgcc cgggattgaa aaatacaaac catccgaact
    11521 ctctggcggt atgcgaaaac gtgtgagcct ggcccgtgcg ctcgccatgg atcctaaata
    11581 cgtgctgtat gatgaaccga ccaccggttt ggatcccatc atgtcggcga tcattgatga
    11641 cttgatcgta cggctcaatg aagaactcaa gattacttct atcgtcgtca cgcatgatat
    11701 gcgcagtgtg tacacgattt ctgatcgtgt ggcgatgcta cactacggtc aggtacgatt
    11761 taatggaact atcgaccaac tgaaatctac aaacgatccc gtcgtccaac agtttatcga
    11821 aggccgcatc gaaggtccga tacaaccggt tttgaaagac ggcatttaat taaggtgtaa
    11881 tctcaaacat gccgctgagt gtaattttaa ataaaagttt gccgggatgc ggcatctgat
    11941 tgctcatggg ttgtatttgg ctcggtgaag aaattggata agacaaaacg gcatattccg
    12001 gttcgagctg cggtgcggcg taggagaatg aatataactt acccggtttg aagcccagcg
    12061 ttttgccggc ttcttcagct ttttttcgga ggacagtctg tgcatttttc aatgcttcgg
    12121 caccgagact gtccattttg gatgttgaaa aatcaatacg gttaatcgtc ataccgttga
    12181 tttgagataa ggctctcatg atgtctggat aagaggttat gatcggtgtc atgatgacgg
    12241 ctgtacggct tacgacatag tcgggctccg ttatggagtt ggaatatcct gagtaagcgt
    12301 tggtggatgg tacactcaac gtatgcgccg gatcgaaacg caaagaacac gggtagtttt
    12361 ttagcgcatc cagtatttta tctgccaaaa gtttgttttg ttcgctggcg gaacgcaaat
    12421 ccgagcgttg tgcatccaca ctcaaggtta tcgtcgcgac gtccggttcg atttcttgca
    12481 tccagtcgta attgatactc aatccaccgc gggctgtatc atacggtaaa ctgtacgaaa
    12541 cattacgtgt ttgactttgc gtcgtatttg caacaatgaa caggccgagc aaaaatttca
    12601 atgagacgcg cataaggttc tcccgttgaa taaaaagaaa aatttgaata aaatacatat
    12661 atcgtaaata agtcaatact acattattat gtagtcataa tagaggaacc atgatcacag
    12721 aggaactgat ttttcaagcg ctcaaaagcg tacaagaccc cgatttgcac aaagacatcg
    12781 tatcgctcgg atttgtaaaa gatatcgtca ttgacggcgg taaagtggcg ttcaaaatcg
    12841 aattgaccac accggcttgt cccgtcaaag atttactcaa acaacaaagt catgatgccg
    12901 tggctaagat cagcggcgtg acctcggtgg aaatcatcat gacgtcgcgg attccggcga
    12961 ataaaggttc catgaatcaa agtaatttat tgcccggtgt taaaaacgtc attgccgtag
    13021 cgagcggtaa aggtggtgtg ggaaagtcca ccgtcgcaac caatctcgct gtagcgcttg
    13081 gtatgttggg tgcgaaagtc ggattattgg acgccgatat ttacgggccg agtattccgc
    13141 tgatgatggg gacttcggag aagccgggtt ccacggatgg tcagcgtatg gatccgatcc
    13201 tgcatcacgg cattcagatg atgtccatcg gatttcttat gccggaggag caagcgcttg
    13261 tatggcgcgg ccctatggtc gcacaggcgt tgacccagct gatgcgcgac gtggcctggg
    13321 gcgaactgga ttatctgatc gtggatatgc cgcccggtac gggcgatgcg cagcttacgc
    13381 tgtcgcaggt tgttcacctt gtcggcgccg tcatcgtgac aacgccacag gatgtcgccc
    13441 tcttggatgc caaacgcggc gtggggatgt tccaaaaagt caatgtgccc attctcggta
    13501 ttatcgaaaa catgagttat tatgtgtgcc ccaattgcaa tcaccgtacg gatatttttt
    13561 cgcatggcgg agcacaaaaa gccgctgaaa aatatgaagt gcctttttta ggtgaagtgc
    13621 cgctagatat cgagacgcgt atggcgggcg atgccggtac gccgatcgtt ttggcgaaac
    13681 cggattcggt gaatgctaaa gcttttatgc aggttgcgcg caatctggct tcgatggtaa
    13741 gcattattca ctacggtacg gcggaagatc ggtcgaaggc gttggggcag aaaacttttt
    13801 ttgccaatac acgcctcaaa gtgatttaag cgaataaata gttgactgca atgatggttt
    13861 cacctatatt gcgtcccgtt tttaagacgg taggtaaatg gcctttttgg aatggaggat
    13921 taggctaatt ggtaagtcag cagtcttgaa aactgccgtc cgaaaggact tgggggttcg
    13981 agtccctcat cctccgctgt tcttttggat gtgatccata tacgaatgca ttgggagagg
    14041 tggccgagtg gcttaaggca gcggtttgct aaaccgccat acggttgtaa agccgtatcg
    14101 ggagttcgaa tctccccttc tccgcaaaag ctcagagtaa tctgagcttt ttttgttgag
    14161 ggagtagatg taaaatatta tgtgtatgta gtatggagtg atcaactgta aaaaagatat
    14221 gtcggataaa atacaattca gtagacattc tcagggaaat agtatgtgta actttatgtt
    14281 taacaagcaa agcgtaattc gctcacttat cttgcttttt ttaatttgta tagtcttaac
    14341 gttttattat gggtatagtg atgaccagac aataaatatt agagaaatga ttctaaggtt
    14401 attgccaaca gtattaattt ttctaatttg ttacgtgcgg ttaagagaat cgttgtcaaa
    14461 tttatttttt gatagcaact atattattat tgagtatcct ttgcaaaaga aaaagataca
    14521 aatattgaaa aaagatatag ttgaaattac tactatcaag aatacagaaa gagacatgaa
    14581 gaaagatggt ggtgtgagta taaaaacaaa aactaatgaa tatcggatat actggatgtt
    14641 tcataaagag atcaaattga ttaaaagaaa actggaagaa attagaccaa tacaacctta
    14701 tagaggaatt tggtctgaga ttctaaagaa atgattaaaa tagcatcatt tttaataact
    14761 ataggtctct tctttgtaag cttagttata tttggaatat atgtcgtggg cacccatagc
    14821 atggattata atatagaggc cttgtacggt gtttttatta ttatgaattt tatgatgaca
    14881 gtgtttgggt taaacacttt cttgtttaaa acgctcacat ctttacaagc cgagaaactt
    14941 acacttttac ttaaattcac gttaccaatt tcagtgttta gttttatttg gtatatagtt
    15001 gatagtatta acggtgccct tgagtcagat ataatttata ttggaaatat agagatattg
    15061 ttttggttgc ttttgttagc agctcttatt tttttaactc tcaaaatttt caattggaaa
    15121 gagaaaaatg aacaatgaaa tacacattcc tcttcctctt aataatacag caaagtgatc
    15181 aatgtgttgg acaaacatcc aaagaagacg ttgcgcgttt gtatgttgct tattttgagt
    15241 tatatatcta tagctcagat attattctcg gaaacaatac ctacaagagc tcaaaaaaca
    15301 gtctagcaac atcaattgtt tctccaaagg aaatcaaaga aaaatatgtt tcttggaagt
    15361 acaaggaaaa atttaatggg ttagaagtgt tttgtgtgag tcatgcatgt aggtactgta
    15421 gtccatacat agtcggtatt gatgatgagt tgaatatcta tagaatgtat ggttttggga
    15481 cgaatataag cgacatttct gagcaaattg atcgcaatta caatgataca acgtactatc
    15541 ttgattttag agatcaaaag tcaatcttaa gcgtagacgt aaaatcattt gtaaagaatt
    15601 acttacgctt ggttgaattt ggagatattt ctgagaactt ggaatttacc agtgaagagg
    15661 ttgatttgat atcgactaca attctcggta gaattacaac aaagtattat gtatctcgcc
    15721 gagacaataa gtttgttgaa atagattgga agtatgcgat aagtcgcgat ggatacgtaa
    15781 gtttatctca aaacgcatca agaaatcatg gggtcgaaat tattgatggt aaagttatag
    15841 atgaaaatta atgtgtttgt agtcacaaat atcatgtttc tgcgctatca cgtcatgccc
    15901 agatcactta aatagcatga ctactcggct tgtagtgttg acgcacttct gcagtatatg
    15961 ttggggttat ctaggaaggt aaaggtaatg aaaatatttt tgtcgcacgt ggcccagaac
    16021 aaatggatgt cccgcaagtt agcgataatg tgaaccttga gagacgggac aatactccta
    16081 catctgtgcc gatcaatatt agtgttacta ccgaggagat aaaatgatac gtaaatcatt
    16141 catttgctat tctctggttt ttattaccac agtgatgagt tataactcaa tgggctcgcc
    16201 ttcaaaaaac cctattttta tcgatattac aggaggcgtt tttccgttgg agaacacgat
    16261 tacagaatca ttagattcaa atgtaaagat cgttattaat accaagttct atttgcgagt
    16321 cttttatagt gattacgtgt tgttcatgga gaaatcatcc aaaaaaagaa tgggaaatcg
    16381 taaactaagg gattatgagc tacatagggt taaccttttg tcaaaaacgg atagcatttt
    16441 ttctatccgt actttgcatg atgaactgtc tgcctataaa actataaaaa attactgcta
    16501 tgcattgcat ggaaaagtgt ttagaagtga ttccattcaa tcacagttaa atacagaagg
    16561 ttggtatgtt tctaatcaaa agtacagcga ccagttgctt gatgaaatag actggattga
    16621 aattgatcat gccaagaatc aggtgaaaaa gatcaatgac tcatttgcga gtttcaaaaa
    16681 tatcctaaaa aaatttgata ataccttggc tccgcttccg cgtatttatg ttttaaaatt
    16741 ggatagttca aattttgcag tttcaaacat gcgtatatgg cttaaagaag attctctgcc
    16801 ggcattattt aaaaaaccac aaatttttaa tctaatatat gatgatcatt ttggcgataa
    16861 gcctgccaaa caatactatg ttgatgggtt ggatgtaata tgctatcatg acaaagtcat
    16921 cggagttaca acagttaatc ccaagtttgc aacgcaaaac aacatacacg ttgggatgtc
    16981 actcaaggaa ttattgaaaa aagcaagtcc agttgatgaa gagaaaatac catctaactg
    17041 tgtaattcgc cttagctgta cgaatgaatg ggggtatctt tcgtattttt ttatccttct
    17101 tattaatgaa aaaatatcaa tgatgctatt ggagtcatga tgaatactgc gtgtattcat
    17161 tatattggta taggttgact tcgtagtaat gagtttgcta aggggtcatt tggcgacttt
    17221 atcataatag ttatgaattg attgaggcgt caaactagta caaaacttag ttaccgttgc
    17281 atgcatgaaa acttgaggcg aggggagaga cttctcccct aatatgatga attgatatct
    17341 gaatgtgata aaatggcggt tgtgaaataa aattagccaa tgatcagatg ctggcaagca
    17401 caatcgtaaa ccggtatgga aaatgttaca ataaaaagta gagtattacc gcattacagc
    17461 gttagagtta cacaaaagct attttctatc aacacatcag tgtaaatcaa aaccagccta
    17521 actatgcaat tagaaacgga cgcggtatgg attgagatga cattcttacc gtgcttgtct
    17581 ttgagttttc attgatatag attagattaa tctcaggagt gacaaactat gcctatccgt
    17641 tccactcttt ttcgtatgat gatcctcgcg atttctagtt tgtctgtcgg cattgcattt
    17701 aatcaatggc acagcagtgg tttcgcatgg gataaattga tacgccgcga ccattcaaaa
    17761 acttatatgc gtatgccgcg tcaggatgca tccaagttgc aaagactttc gctggacgaa
    17821 gcctatacgc tttttaaaac caaagccgga ttatttgtgg acgcacgcca ggaagcgatt
    17881 ttcaaattgg ctcacatcca gggcgccgtg tcgtgttatt atagtcgcgc aaaggaacta
    17941 cccatagtca atgaatggga aaaaaataag acattgataa cctattgcgg cggaccgcgt
    18001 tgcgatcagg ccgaccgtct cgccatgcag ttgatcgaaa tgggttttac caatgttttt
    18061 gtttttaccg gaggaatgga agaatggcgc gctgccggtt atccgatcga agaacaaaaa
    18121 cgcagtactc accctgaata aaaaaataat tatggcttgg aaaagtcata taaaaaccgc
    18181 gttacgtcta attctcggca tcgtattttg ttatggcgcc ttagataaaa tcacatcgcc
    18241 atacgccttt gccgccgcga tagacgccta tcgtatttta cccgccgtgg tgacaccgat
    18301 ttcagccgtc tggctagcgt ggctggagtt attcatcggt atagcactca tggtcggata
    18361 ttggactcgc gcggccgccg gatgggccat cctacttttg agtatattta tttttgctat
    18421 cacctcagcg ctcctgcgcg atctggttat accgtgcgga tgctttgatg tgaatgatcc
    18481 ggatgaagtg ataagttgga aaaaaatagc cgaagatggc gttttactga tgatcgcggt
    18541 gtatgttttt atatcgccgt atgcacggtg gtccttatca aaagaagagc gtctatcgta
    18601 cgacggcaaa ctttcctaac tttttctttc ccgacacgac cgcttcatat atatacacgc
    18661 cggatgagat tctcttgccg tttttatcct ttaaatccca tcgtacccca ccgtcatttc
    18721 cgctttcggc gatctgtttt ataaaacgtc cgtcaacgga ataaatccgt accacggtct
    18781 tatcgggaat acctgcgaag gtgatataag gcggatctat atcggcgcga tggggattgg
    18841 gatacacaaa cggatcatct aatttttcaa cgctgaataa taatccggca cgatagccaa
    18901 aaacagaatc tatcggatat ccttcgctgt ccttgacgcc gtgtgcgtct atcgtaaaag
    18961 ctaaaccgat cgggcctaaa tatttttttc cgtcaacgtc cagaaacact tccgtattgg
    19021 atgacagggg aacgatactt gccgagacaa ttgagaaatc ataattcata cgatacgtcg
    19081 aggtgcgtgt cgctgtagcg atgtccacgg atttattgaa aatcaaacga atgcggttgc
    19141 gaccttcgta tgtcgcctgc acgatgtaca aaggtgtgcg ttgtgtaact ccgtcataca
    19201 aaaacgtacg ggtggcagtg tccgatggcg tgccatcggt atcgtgctgt gaataaaaaa
    19261 acaatcggtg cgtaccggat gcaagttgcg taaagtgaag catcacggct tgaccgcctt
    19321 cgacaggtat cgcgctcgcc ggataatacc ggtcatccag caaataatga aaattctgac
    19381 tcaggctttc gttgtgtatc ggttcagaaa accgggctcg tataaaattg gcattataca
    19441 tttcgaccga tacaactgat ggtttcggat tggggcgcac aaaaaaaaga ccggatacat
    19501 cggatgaggc gttgccgtag cgcgcttgta ttctgtaata atac
//
LOCUS       DAOQNQ010000030        19506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_978_length_19506_cov_8.318493, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000030 DAOQNQ010000000
VERSION     DAOQNQ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19506
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_978_length_19506_cov_8.318493"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4743)
                     /locus_tag="PK754_03605"
     CDS             complement(<1..>4743)
                     /locus_tag="PK754_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNB08618.1"
                     /translation="ASYNGLSDETGLITVIGGAPDSIYEISSTSVSLTPTQTTTLKVK
                     LTDDDGNGLQDSVVYWTIQTGSGALSLASDSTDELGVSQVVFTAPTTSGVSTVRAYIT
                     SLSTDSGRFTITTNPAVLAYYTIIPSTNNDTVGVAITATVTAYDIFNNTLDDDTTRVQ
                     FDVIGSVTGLNGSGVGINPTNDTLTNGQLTVVVTDSVKETLQIRVTSLLDTTKRTATN
                     LITLRAAAPSIVEFYPDSLTNVQSGQFGQPLLDSLALVVKDQFGNTVNDTVTVQWFAT
                     SDGTASPVIRTTDNDGIARSLWTLRTGASSYDTMYAVIPVTGDSLQFIANLLPTGEDT
                     LIRVSSLVNSDTVNQMLPDTFRVLVRDQFSNPVSNTAISFTAYSYPSGSDSTGFIRGA
                     GNYVTTLDTVTDINGYASAIFHMGTKVGQYIIRASNPSLLNPAVFDTIQATFGLPGEL
                     RLLSGNAQTDTVDQIVDTLRVQVYDRYGNVVNDTARVIWEAINNSVSVLNQGLVDAVS
                     IDSVSGTPTFRDSALAFTQGIAKANWRLKDLTGSDSVRAFIPGIDTVLFTATTVPDAP
                     FAIIKTEGDSATVVPLGGLVTLKTRLEDQFGNVISGKYVRQNVLAGSVIFVGGDSAMS
                     NAQGISLIELQLGGGDSATVQATTSGLVGGPIFTTYALRYVVGSLKPTIALADTEATF
                     TAAFINYGPHPVALDSNQTRVRFSDGVTTFRSLLSYGDTVIRAHGQDTLQFRTDTIPS
                     SFGGKQFNVRFDLRGQVASVSDTLYGTLETGANVLNILGVDVISVNTPAPKVYGRGDT
                     ITVQLQVYNSSNVTLTNLNYGLLTSKSGVFDLISSIDATPFAPRSTSVFNITAVVRAS
                     APLGQIVVNGFYSGSNGGVGVSDTLANTMDTIIIQSDASLRYVAATLSPDTVSEGQSV
                     AFRISVINDSLASVILDKANTYLRFGGDSTYLTSSQVVPGSGASTTLLFDPLTITNAT
                     SGRYQGVLVLNGTENGGLFDTTLFTGADSLTVQTTVTTASLEFDSVEIALDTTAQYRD
                     SVALRVRIVNNAQASAVIDSIILDATRNLATISGYSVVGTGSLPDTIAGNSSSVFTLY
                     ALISSVADTGSIVFDGRIVAHDINSGNTVNLSPVTDKDSIFVRKRATLSIISVVSPSH
                     DTVAVGQNNIPFVVTIANQGGSPATISDLQLVFKRGLYDTTLTQTLPDTLYGYETRAY
                     NFTAHVLSNSATGLDSIGAYVSGSDQLNQRMISLTSTGRDTLLILSNSLIALNSVQTT
                     PTLVSRGQDSILVRVSVTNLSSSVGRIDTIQLKFFDEAVSLLNTGFDVTPYSDLSDTV
                     SGNTTKSFLFKVNIDAAADTGITTIDAVLKGRDEVSGTSVYDSLAVSADQWTIQIPAQ
                     LTVDSVWALPATVSQNQQNIPVNVVIRNTGVATARVDSVRLRYYYGSTLSTSDFVDSL
                     TVPLGTITLVGGDSALYLFNTDALSSAASGTVRFYAVAFGQDNNSLQARVDTSRLRYD
                     SVLVQTRPVLSYTANTLTPDTVTIGDAFNYRLDISNSGEANVILTPSTQLILRDVDSL
                     KVNLQDTVFIASGYTRTLDFSTALIGLRI"
     assembly_gap    4744..4843
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4844..8384)
                     /locus_tag="PK754_03610"
     CDS             complement(<4844..8384)
                     /locus_tag="PK754_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08619.1"
                     /translation="MWTDSKTSTAAPYATYQWVDASAGTVITPTTGSTTGLVNIGFNF
                     VFYGNTYSQVDISSRGFLSFQNLQSNDFSTNNDLSVAGGPDSIIAAYWDAITCVTASR
                     IYYQTIGTAPHRQFVVSWNSVRDGAQNLIFQVILYETSNLIKIQYQTVGTATGATATV
                     GIDSASGTASQYSFNTANLQSTMAVLFHTNATASASATISPSSVRTNTTLNSFTYSIY
                     VNGAIDSLARIDSVLIRNPFTTKSATVTDISVDGSSKFFINSSSRPSALGLSSYDFAT
                     WSYNTTNDSISVRTNVFGIRDSIKVTFTVDAENILGNVNFPSSVLAPLVSETRLSVSG
                     SPSVSVIPDTLYTLSVKWYNQEPSGLAVDTSIIADDSLRVFAYGYDRNGNFLRRVKVN
                     WSSSGTMGFLAGATSGSDSISFHGRRVSSGYISADSAGFHDQTGLITVTVGDTSFAVL
                     RSQANNGGLRYDTLSILTTADTSMQFYLAGYDADSNYTGDLVAYFDTVYGVSSGLSGF
                     TADSSLGVVYNPTLVGSGYMRARYYNNASGYIADTTGLITVLHGDTARLVIEDARFSS
                     QAVGADTLTADQTLRLYAASYDGDGNFIGNLQSTAWSLSGGIGSLNTALSDTVILTAS
                     NAGSGSVRASYNGLSDETGLITVIGGDTSYILIRTGARNSGNVFSSALTLKADSSLVL
                     HAAGYDADNNYLGPVYVSWSLAAHTVPSLSNFTPVSDTVLTYVPALVGSDSIIAQMLG
                     DALRDTTGLITVVPGDTAMLLVETMRNGNGVSFPNLVSTADSVVTGYALYRDIKGNVI
                     DSATSGIVWSVSGRIGSIVQEGLDSAVLRLDTVGVGQLRAVAGGFSASSGFIRVSPGI
                     LYSVKLMRDTLLLGDEVDDFSISVGDSIKLYAAAFDSNGNFRGNIASSYAAHSGTLQS
                     SRLFIRSDSTSVVFWPSTASTGRIYVTSNYLGISDSSGVVTVTNTAAVAGIRINSEAH
                     NTGTEVLALTVTSDEDTTLHIASYDAFGNYLGDVAGRFSVTGSLDSLDLSPQLNTSAA
                     VYNARVAGGSGRITAEYSGFRDSTGLVSVTAGGIAFVQVQTAANDIASTLSSKSATTD
                     SNGITLYAASYDADSNFVRLVQATWSVEGGIGSVSSPGTSTSFNARRTGSGRVVADTS
                     VFEAKTGLITVTAGDTSFAVLRSQANNGGLRYDT"
     gene            complement(8483..9001)
                     /locus_tag="PK754_03615"
     CDS             complement(8483..9001)
                     /locus_tag="PK754_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08620.1"
                     /translation="MKLFFKLSALVFSFNLFLPLDLFSQSGSYVIDQTEWTEGKSAEL
                     RLAISIKNGSGKIIEIGNALNDEIQIVSIEHNTASLWMTTNQALFDKKISGTALVQIV
                     EKRAKIEFNDALKSGDKLVIKLALAVPTAHALKSHSDKNHSLDVTVSKDRNSSSVKLT
                     NSQTIKVKADAP"
     gene            complement(9245..10093)
                     /locus_tag="PK754_03620"
     CDS             complement(9245..10093)
                     /locus_tag="PK754_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNB08621.1"
                     /translation="MPKSRLPHLDETGVPLKAYDNGSFLHSPDGRTLRVISEMHEPYA
                     RFKKYGIRDTIVFYGSARILDPKDAKRKLLVLQKEANNQLKPSVSLRRQIERVQSDLE
                     MSAYYRDAMKLAEMLTRWSSKLDGGKRFIVCSGGGPGIMEAANRGAKKAGGPTIGLNI
                     SLPFEQIPNPYISPELNFEFHYFFMRKFWFAYLAKALVVFPGGYGTLDELFELLTLVQ
                     TKKIEKPLAILIYGKKYWDEIIDFEAMAKHHMISPEDLQLFSYADKPEEAFEYLREKL
                     ERYYLK"
     gene            complement(10136..13198)
                     /locus_tag="PK754_03625"
     CDS             complement(10136..13198)
                     /locus_tag="PK754_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNB08622.1"
                     /translation="MKNIIMILLYAFLGTYICAQNQSTFSPFQELSEPPVLTFESGLH
                     LDRVNAVRYSPDGKEIVTASWDKTIRIWDAWTGQLLRSLRVPGYAGTEGQIFTMDISP
                     DKKYILIAGSSVGQRYNTLTANYIGDYVLMLDYVSGAVVDVAAHHKQSIYGVRFSPDG
                     KKVVSVGGSFDNRINQYDVNTNTGHLVLRDSAVTTQEAVKAGLCNWGFLGICDHQVVG
                     TGFVDNGNAWLAADYHGLVFRKKAGENLRVIGKAVDGPCRSIATDAKGKLAAIGDFRG
                     RVRIIDALQNEGDNIVANIMTFKSDMITAMVFDTAGEQLAVCVGNEVRVYSLTSEADG
                     RLSKKHVISFRQHDRPVFAVAFSPDGKHVVSAGGLHNEIHVWNVADGERIMTLGDVSK
                     SGKISAIGIHRDHPNVIGIGETFNFYEKLNDYGTISKTFNLSTFEFKTDSVEQSLFEN
                     ARLAQLRAPVSLPDIKWLLDDRPLCAMKLQNGKLLLGTYYGIFIDSPANMLSYDGART
                     IGLAESPDGRRFYVGLESGEIKIYDENKMTYIASLYVTSDNEWIIWTTDGYYAASKRG
                     AQRVGWQINKGLNMTPKFYPFEQFDLRLNRPDIVLQRLGVADSARIAALYGAYQRRLK
                     RLGLTESQLGSSLQVPDIQIKGDVTETDLREVILTISAYSKEAPLERLMVNVNDVPLH
                     SSYGKKIGDGYLNQWNETQRIPLTQGRNKIQVSVINTAGIESLKETRYVHCRSQDTDP
                     ALYVIAVGVSHYTDREFDLQFAAKDAGDITDFFRNALASNKLPYSKLHIKTLLNEQAT
                     KDSIIQVRNFIANCKPQDGVVMYFAGHGIPDSTVDWVFATYDIDFYKASEKGITFAEM
                     EGLLDGIAARKKLMLVDACHSGERDRDETEIVDNAKDTKAQQVRAFKNVKTKSKSGVG
                     LVNSFELMQHLFSDLQRGSGAVVISAASGTEWAAEGDIGGKTIKNGVFTYGLLEGLRT
                     MAADRDKNGKITVTELRDYVMQSVRTLTRGAQHPTARKENLEFDFRVY"
     gene            complement(13268..15025)
                     /locus_tag="PK754_03630"
     CDS             complement(13268..15025)
                     /locus_tag="PK754_03630"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08623.1"
                     /translation="MFRSLFIALFLSVTLQAQLKIAPGMPMYNAPADSLCEYGRAGVE
                     MKSEMMALTYYNHAIDKYPQHPRAYAEVAMMYAEFGKFEVAKEYLQQAESIDNKYPMT
                     LFARFTFIREEARSKYKFDQMPKDVASAVVEAGRAFLAVKDTSMNDRKAYIKQFLPTY
                     QLVSDDIEAFKMYRLALQDKENNNFEDAFKKFETIITRVQNTGNTGIISSVSTEAGIC
                     KFKLGEYPAAKTWFQKSIATPEAEGLAYKFMAQILYQIDKNVVEAEKLCLSGLSKNLS
                     GSDEIKTYLCNIYFTDGKDAFDKKNYWAAATNLDKLVKYDDKNAQAYGYLLYALYQTK
                     QYTKAGIAVQKLLELNEAEENLALYPNLTDIQKFCKNPVGAAPNFKYNQIVADSADLK
                     IKTLDLMYQLKMYDKAVEESQNALAMYSRIGHKAGMAWAYYKLGMAYHYKSDVANAKL
                     NYQKSIDTKPITGGVYSNLYQIINSENPAEAHKILDSGYKLFPDHDRLKGLMSDFYWN
                     KAWEEMSAQNYNNAIDLSNKSNEMSENAVAYFVLGYSYYALGKGSDALYCMQRAKQLD
                     PEMPKQYPQIDQIINYYSK"
     gene            complement(15221..17011)
                     /locus_tag="PK754_03635"
     CDS             complement(15221..17011)
                     /locus_tag="PK754_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB08624.1"
                     /translation="MFKRLKPYLWKYRVYWISGFVLVILKNLFQSTGPQLVREAIDTL
                     APGTDPSTFPLYNQIIHSVELQLGVAIVVAYIAAYLLVELLHGIFLYLMRQTLIVMSR
                     KIEYDLRSDFFSHLQKLHVQFFQYAKTGDLMSRLTNDLANVREVLGPGIMYTANTIVA
                     FIYVVPMMIHISPMLTLLAFLPLFLLSFAVGRLSGVIMTRSQKVQEKLSDISSAAQEN
                     FSGIRVVKSFVQEAAEIKKFENLSHEYIQLNMSMVRIRGIMMSSVILTIGLSVAVLLW
                     YGGSLVIDGVMSIGEFTAFNFYLAMLIWPMVAVGWVINIFQRGSASMKRMNAIFDEQP
                     AISDKNNQSNGHSDIAGKIEFRNLNFSYRDIPVLKNISICIHPGMTLGIVGATGSGKS
                     TLVNLIPRLYDIPDGMLFIDDRDINTIPVSILRQHIGMVTQDNFLFSDTIYQNIVFGV
                     DNPPTDKVIWAVDTAQLRSNIEDFPEKYETIIGERGITLSGGQKQRLSIARAVIREPK
                     ILILDDALSSVDTHTEDEILKRLKILMKNRTTLLVSHRIQTVKHADLIIVLKEGAIIE
                     TGTHDDLVEQNGTYAAMHERQLLEEEVANI"
     gene            complement(17018..17605)
                     /gene="nadD"
                     /locus_tag="PK754_03640"
     CDS             complement(17018..17605)
                     /gene="nadD"
                     /locus_tag="PK754_03640"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNB08625.1"
                     /translation="MEKIGLFGGTFNPIHAGHLIIAEIIRDHLGLSKVVFIPSARPPH
                     KTSENVISFHHRKTMVDLAIEQNEYFQCSDIEEKLSGLSYTVHTLEFLRKENPKNEYF
                     LMIGSDSLLDLPNWKTPERLTELSKIVVYPRMGFDFDKGELQFTKEAICIDKPVIEIA
                     SSWVRERLLRNQSVRYIVPNAVLSYIKKNNLYTKE"
     gene            complement(17615..18451)
                     /gene="bamD"
                     /locus_tag="PK754_03645"
     CDS             complement(17615..18451)
                     /gene="bamD"
                     /locus_tag="PK754_03645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNB08626.1"
                     /translation="MLRKIFVLAMACSFLQSTGCSKKDVTQYMTSREHFEYAMKFFNK
                     KNWTSAQEQFSLITYKYSGSDIADDAQFYLAESYYRQHDWVSASSEFERMVSSYPKSE
                     FIEKSMYQLALCYIELSPGFALDQKFTYQAQNAIQNFIDLFPRSELRTEMDKHYKSVK
                     DKLARKHFESAQIYRKISEFEAAIVYYDQVITDYYDSPYAMQAKYWKGYCNYKIEEFQ
                     KAQILLKKFIDEYPLEKEWVTDALETLEDIKKDELKAQAKAAKEAKLVKPEQSTSVKS
                     AN"
     gene            complement(18504..19277)
                     /locus_tag="PK754_03650"
     CDS             complement(18504..19277)
                     /locus_tag="PK754_03650"
                     /inference="COORDINATES: protein motif:HMM:NF024628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HNB08627.1"
                     /translation="MCRLMGYAGPNPVSLQNVILDAPNGILRQSVCDRDSCKPNDDGW
                     GFGFEENGQFHVIKEASAPRNDKLFLDTAHRPFKRVMLHIRRASVGGASKVNTHPWIY
                     HQDIIFAHNGSVYGIENVMDSALAKIDPALSGHITGNTDSEYAMHLFLTYLRKYNPAQ
                     TLDIHFVRTSLQHTIADLREISRRADASRKPQLNFMMLTRDYLIATKVMHSLGYYTSY
                     QGGTLVTSEALDNNDGWTLMKEETMIVVSRNGDYALYDI"
     gene            complement(19277..>19506)
                     /locus_tag="PK754_03655"
     CDS             complement(19277..>19506)
                     /locus_tag="PK754_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08628.1"
                     /translation="EGVVQQLSGDSKNADRFRPFIETLQGKYLDSFKRMQYQLDQNKK
                     PATILKIEYDRLANSEKFKVYLKHCFELIKH"
BASE COUNT         5561 a         4604 c         3838 g         5403 t
ORIGIN      
        1 aatgcggagc ccgatcaatg ccgtcgaaaa atcaagcgtg cgtgtatagc cggatgcgat
       61 aaatacagta tcttgtaaat tgaccttgag ggaatccaca tcacgcaaaa tgagctgtgt
      121 agacggcgta agtattacat tggcttcacc gctattggaa atgtctaatc tataattaaa
      181 cgcatcaccg atcgtaaccg tatctggtgt taaagtattt gcggtatacg agagaacggg
      241 tctggtttga accaatacgc tatcataacg taaacgtgat gtgtccacgc gcgcttgtag
      301 actgttgttg tcttgaccga aagcaacagc ataaaaacga accgtacctg aagcggcaga
      361 cgacaaagcg tctgtattga acagatataa agcgctatct ccgcctacaa gagttatcgt
      421 cccaagaggg acggtaagcg aatcaacaaa atccgaagtc gacaaagtac ttccatagta
      481 ataccgcaat cgaacactat caactcgggc cgtcgcaaca cctgtatttc gtataaccac
      541 attaacaggt atgttttgct gattttgaga aaccgtcgcc ggtaatgccc agacactatc
      601 aacagttaat tgtgccggta tctgtatcgt ccattgatca gccgaaacgg caagcgaatc
      661 atatactgat gtaccggaaa cttcatccct tcccttcaat acagcgtcaa tcgtcgttat
      721 tccggtatca gctgccgcat caatattaac tttgaatagg aatgactttg tcgtattacc
      781 ggataccgta tcgctgagat cgctatatgg cgttacgtca aacccggtat taagcaacga
      841 taccgcttca tcaaaaaatt tcaattgaat ggtgtcaatt cgtccaacgg atgaactaag
      901 attagtcacc gaaacacgta caaggatact atcctgtcca cggcttacta aggtcggcgt
      961 agtctgtacg ctattcaatg cgatcagcga attgcttagt atcagcaatg tgtctcgacc
     1021 cgtagatgta aggctgatca ttctttgatt gagttgatcc gaacccgata catatgcgcc
     1081 gatcgaatcc aatcctgttg cggaattgga taaaacatgt gctgtaaaat tatatgcccg
     1141 tgtttcgtaa ccatacaatg tatccggcag agtttgcgtt aatgttgtat catataaacc
     1201 tcgcttgaat acaagctgaa ggtcagatat tgtagccggc gatccgcctt ggttggctat
     1261 cgtcaccaca aagggtatgt tgttctgacc gacagcaact gtatcgtgcg aaggactgac
     1321 taccgatata attgaaagcg ttgcacgttt acgaacaaat atagagtctt tatctgttac
     1381 cggtgacaga ttaactgtat ttccgctgtt gatatcatgc gctacaatac gcccgtcaaa
     1441 tacgatactt ccggtgtccg ccacagacga aatgagagca tatagcgtaa agacgcttga
     1501 gctgttacct gcgatagtat ccggtagact accggtacca actacagagt atccgctgat
     1561 agtagctaaa tttcgagtag cgtctaagat gatagaatca ataacagccg aagcctgtgc
     1621 attattgacg atgcgaacac gtaatgcaac ggaatcgcgg tattgcgcgg tggtatccaa
     1681 tgcgatctca acgctgtcaa attcaagact ggctgtcgtt acggttgttt gcacagtaag
     1741 actgtctgca cccgtaaata atgtcgtatc aaataatccg ccattctccg taccattcaa
     1801 caccaataca ccctgatatc taccggatgt cgcgttggtg atcgtcaacg gatcaaaaag
     1861 cagcgttgta gatgctccgg aaccgggaac tacttgagat gatgtcagat aggtactatc
     1921 gccgccaaag cgcagatagg tatttgcttt atctaagata acagacgcca gtgaatcatt
     1981 gataaccgat atccgaaatg cgacagactg accttctgaa acggtatccg gcgacagggt
     2041 cgctgccaca taacgaagac tcgcatcaga ttgaatgatg atggtatcca tcgtattggc
     2101 taacgtatcg gaaacgccca cgccgccgtt tgaaccggag taaaatccgt tcacaactat
     2161 ctgtccgagc ggcgccgatg ctctaactac ggcggtgata ttgaatacac tggtagaacg
     2221 tggtgcaaac ggagtggcat caatcgagct gatcaaatca aaaactcccg acttgctcgt
     2281 caatagcccg tagttcagat ttgtaagtgt aacattgctc gaattataaa cctgtagttg
     2341 aactgttata gtatcgccac ggccatacac cttcggtgct ggggtattaa cagatatcac
     2401 gtcaacaccg aggatattca aaacatttgc accggtttcg agcgtcccat atagtgtgtc
     2461 actgacggac gccacctgac cgcgcaaatc aaaacgtaca ttaaattgtt ttccaccgaa
     2521 ggaactcgga atggtatctg ttctgaattg caacgtatct tgaccgtgag cacgaattac
     2581 ggtatctcca tacgacaata acgaacggaa agtagtcaca ccatcagaga aacgaacgcg
     2641 cgtttggttt gaatccaaag caacaggatg tggtccataa tttataaatg ccgccgtaaa
     2701 agttgcttcc gtatcggcga gcgcaatcgt aggcttgagc gatccgacaa cataacgcaa
     2761 ggcgtatgtt gtaaaaatag gtcctccgac caaaccggac gttgttgctt gaacagtagc
     2821 gctatcaccg ccgccaagct gcaattcaat taacgagata ccctgtgcat tcgacatggc
     2881 cgagtcgcca ccgacaaaaa tcaccgaacc ggcaagtaca ttttggcgaa catatttacc
     2941 gctgataaca ttaccaaact gatcttcaag gcgtgtttta agtgtgacca aaccgccaag
     3001 cggtacaacc gttgcagaat cgccttccgt tttgattatg gcaaaaggtg catcgggcac
     3061 cgtcgtagct gtgaataata cggtatctat tccgggaata aaggcgcgta cactatcgga
     3121 ccctgtaaga tcttttaatc gccagttcgc ttttgcaata ccttgcgtaa atgccaatgc
     3181 cgaatctcgg aacgtgggtg ttcctgaaac ggaatctata gatacggcat ctaccaggcc
     3241 ttgattcaaa acgctgacgg aattatttat ggcctcccaa attacgcgtg ccgtatcgtt
     3301 gactacattt ccgtaacgat catatacctg aacacgtaat gtgtcaacaa tttgatctac
     3361 cgtatccgtt tgtgcattac ctgaaagtaa acgcagttct cccggtaaac caaacgtcgc
     3421 ttgtatggta tcaaatactg cgggattgag cagagacggg ttggatgcac ggattatata
     3481 ttgaccaact ttcgtgccca tatgaaatat agccgaagca tagccgttga tatccgtaac
     3541 cgtatctaat gtagtaacat agtttcccgc accgcggata aatcctgtac tgtccgatcc
     3601 ggatggatac gaatacgccg tgaatgaaat cgccgtattg gacaccggat tgctaaattg
     3661 atctcttact aatacgcgga acgtatccgg tagcatttgg ttgacggtat cggaatttac
     3721 gagtgagctg acacgaatta atgtatcttc gccagtcgga agcagattgg ctataaattg
     3781 caatgagtcg cccgttacag gtatcactgc atacatcgta tcgtacgatg acgcgccggt
     3841 gcgcaatgtc caaagtgaac gcgcaatacc gtcattatcc gtcgtgcgaa taacgggtga
     3901 tgccgtgccg tcggatgttg caaaccattg taccgttacg gtatcattga cggtgttgcc
     3961 aaactgatct ttaacaacta atgccaaaga gtccagaagt ggttgaccaa attgaccgga
     4021 ttgtacatta gttaagctgt caggataaaa ctccactata gacggcgctg cggctcgcag
     4081 tgtgataaga ttggttgccg ttcgtttagt tgtatccagt aaactcgtaa cgcgtatttg
     4141 taaggtttct ttgaccgaat ccgtcacgac gactgtcaac tgtccgttgg ttagcgtgtc
     4201 gtttgtagga ttgatcccca cgccgctgcc attcagccct gttacactac cgataacatc
     4261 aaactgcacg cgcgtggtat cgtcatctaa tgtattattg aatatatcgt acgccgtgac
     4321 tgtcgcggtt attgcaacac ccaccgtgtc attattggtt gaaggtatga tcgtataata
     4381 cgccaataca gccgggtttg tggttatcgt aaagcgaccc gaatcggtgc taagacttgt
     4441 tatgtatgca cgcactgtgg acacaccgct tgttgtaggc gctgtaaaca caacctgcga
     4501 tacgcctaat tcatctgtag aatcactggc taaactgagt gctccacttc ccgtttggat
     4561 ggtccaatag acgacactgt cttgcaatcc atttccatca tcatctgtca gtttaacttt
     4621 taacgttgtc gtctgtgtcg gagtcaaaga tacagaggta gatgatattt cataaatcga
     4681 atccggtgcg ccccctataa ccgtgatcaa gcccgtctca tcgctaagac cattgtaact
     4741 tgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcgtatc ataccgcaaa
     4861 cccccgttat tcgcctgact gcgaagcacc gcaaacgacg tgtcgccggc cgtgaccgta
     4921 atcaatcctg tcttcgcctc aaataccgac gtgtccgcta ccacccggcc tgaacccgtg
     4981 cggcgcgcat taaaactcgt actcgtaccc gggctactca cactccctat accaccctca
     5041 acactccaag tggcctgcac caaacgtaca aaattactat ccgcatcata ggaggccgca
     5101 tacagcgtga tcccgttact gtccgtcgtc gcactcttgg aggagagcgt actggctata
     5161 tcattcgccg ccgtctgtac ctgcacaaat gcaatcccgc ccgctgtcac actcaccaag
     5221 ccggtgctgt ctctaaaacc gctgtattcc gccgtaatcc ggccgctacc gcccgccaca
     5281 cgcgcattat ataccgcggc cgaggtattc aactgcggcg ataaatccaa actatccaaa
     5341 ctgcccgtta cactaaaacg ccccgccaca tcacccaaat aattaccaaa cgcatcatac
     5401 gatgcaatat gaagcgtcgt atcctcatcg ctcgtcacgg tcaatgccaa cacctccgtg
     5461 cccgtgttat gcgcttcact attaatccgg ataccggcca ccgccgccgt attggtcacc
     5521 gtaaccactc cgctgctgtc gctaataccc aaataattac tcgtaacata aatacggccc
     5581 gtagatgccg tactcggcca aaataccaca ctcgtactgt cactgcgtat gaaaagacgc
     5641 gaggactgaa gcgtaccgct atgcgccgca tacgaagacg ctatattacc cctaaaatta
     5701 ccattactgt caaacgccgc cgcatataac ttaatactat ccccgacact gatactaaaa
     5761 tcatccacct catcccccaa aagcagcgta tcacgcatca gcttcacact gtaaagtata
     5821 cccggactca cgcgtataaa tccgctcgaa gcgctaaatc cgcccgctac cgcccttaac
     5881 tgacccacac ccaccgtgtc caatcgcaat accgcgctgt ccaatccctc ctgcacgata
     5941 ctccctatac ggcccgatac actccataca ataccgctcg tcgcactatc tatcacatta
     6001 cccttgatat cacgatacag cgcataacct gtcaccacac tatcggccgt actcaccaaa
     6061 ttcggaaaac ttacgccatt cccattacgc attgtctcca ccaaaagcat cgccgtatca
     6121 ccgggaacca ccgtaatcag tcccgtcgta tcccgaagtg catcacccag catctgcgct
     6181 ataatactgt cactacccac cagcgcaggt acatacgtca gcaccgtatc actaacaggc
     6241 gtaaaattcg ataaactcgg caccgtatga gcggccagtg accaactcac atacacagga
     6301 cctaaataat tattatccgc atcataaccc gccgcatgaa gcacaaggct gctatcggcc
     6361 ttaagcgtca acgcactcga aaatacattc ccgctattcc ttgctcccgt acgtatcaat
     6421 atataactcg tatcgccccc tataaccgtg atcaagcccg tctcatcgct aagaccattg
     6481 taacttgcac gcacactccc tgaacccgca ttagaggccg tcagaatcac cgtatcggat
     6541 aacgccgtat tcaaactccc tataccaccg ctcaaactcc aagccgtgct ctgcaaatta
     6601 cctataaaat taccatcccc gtcataggaa gccgcataca aacgtaatgt ctgatccgcc
     6661 gttaatgtat ccgcccctac cgcctggctg ctaaagcggg catcctctat cacaagacgc
     6721 gccgtatccc catgcaatac cgtaataagt cccgtcgtat ccgctatata accggatgca
     6781 ttattgtaat aacgcgcacg catataacca gaacccacca gcgtcggatt atacaccacg
     6841 cccaaagaag aatccgccgt aaaaccgctt aatccgcttg aaacaccata caccgtatca
     6901 aaatacgcta ccaagtcacc cgtataatta ctatccgcat cataacccgc taaataaaac
     6961 tgcatgctcg tatccgctgt cgtcaaaata ctcagcgtat cataccgcaa acccccgtta
     7021 ttcgcctgac tgcgaagcac cgcaaacgaa gtgtcaccga cggtaaccgt aatgagaccg
     7081 gtttgatcat gaaaaccggc cgaatcagca gaaatataac cactgcttac acggcggcca
     7141 tgaaacgaaa tgctatcgga tccgcttgtc gctccggcca aaaaacccat cgtacctgaa
     7201 ctcgaccaat tgactttaac acgacgcaga aaatttccat tccgatcata accatatgca
     7261 aagacacgta gtgaatcatc tgctatgatt gaggtatcaa cggcaagacc ggatggttct
     7321 tgattgtacc atttgacaga taaagtgtac agtgtatcag gaataactga aaccgaagga
     7381 ctcccgctga ccgataatcg tgtctcgctg accaaaggcg caagtacaga tgaagggaag
     7441 ttgacattgc ctaaaatatt ttcagcatcg acagtaaatg taactttgat agagtctctg
     7501 attccaaaca cattggtacg aacgctgatc gaatcattcg ttgtattata actccatgtg
     7561 gcgaagtcat acgatgaaag accaagcgcc gatggtcgcg aagaagaatt gataaaaaat
     7621 ttcgatgaac cgtcaacgga tatatctgtt accgtggccg atttagttgt aaatggatta
     7681 cgtattaaca cggagtctat tcgcgcaagg gaatctatcg ccccattcac ataaatcgaa
     7741 tatgtaaacg aatttagtgt tgtgtttgtt ctaacgcttg acggagaaat cgtagcactg
     7801 gcggacgccg tggcattggt atgaaacagt accgccatcg tactttgtaa attggccgta
     7861 ttaaaactat attgtgatgc ggtaccacta gcactatcaa ttccaacagt ggctgtcgct
     7921 ccggtagcgg ttcccactgt ttgatactgt atcttgatca gattggaagt ctcatacagg
     7981 atcacttgga atattaaatt ttgcgcacca tcccttacgc tattccatga gacaacaaat
     8041 tgtcgatggg gcgctgttcc gatcgtctga taatatatac gtgaagctgt cacgcatgtg
     8101 atagcatccc aatatgctgc aataatgcta tccggcccac cagctactga aagatcattg
     8161 tttgtagaaa aatcgttgga ttgaagattt tgaaaactga gaaatccgcg tgaactaata
     8221 tctacttgag agtaggtatt accgtaaaaa acaaaattga atccgatatt taccaaccct
     8281 gttgttgagc cggtcgtggg agtgattaca gtaccggctg atgcatcaac ccattgatat
     8341 gttgcataag gtgcagcggt tgaggtcttg ctgtccgtcc acatgtaggc acggtttgaa
     8401 ccaccctgtc ccgcagatac aacaaacgag aaaaaaagga ataataaaaa actatatttg
     8461 gttgcgcgta tcaaggaatg catcaaggtg cgtcagcttt aactttaatt gtttgcgaat
     8521 tggttaattt cacagaactg gaattacgat ctttacttac ggtaacatct aaactatgat
     8581 ttttatcaga atgtgatttt aacgcatgag cggtgggcac agccaatgcc aatttgatga
     8641 caagcttatc accggatttt aaagcgtcat taaattcaat cttagcacgt ttttcaacaa
     8701 tttgaaccaa tgccgtaccc gaaatttttt tatcaaataa tgcttggttt gtggtcatcc
     8761 atagtgaagc agtattgtgt tcaatggaaa cgatctgaat ttcatcattc aacgcattcc
     8821 caatctctat gatcttacca cttccgttct ttatggaaat ggccaatctc aattcggcgc
     8881 tctttccctc tgtccattcg gtttgatcaa taacataact tccgctttgg gaaaacaaat
     8941 caagcggcaa aaataaatta aagctaaata ccagagccga aagtttaaaa aaaagtttca
     9001 ttcttctctc atactgaaga aagcgattag aaaaaattaa cacaccgaag acttgattta
     9061 ctgaaatgaa ttagcaaatc aaacaaaaag tgacgaaccg atacacataa aaaaataata
     9121 gcaaaaacac gccgtctttg caaacaaatt caataaaatg ataatgatag ataaaaacta
     9181 agccgttgag atatatcaca acggcctata aaaaatactt agatataaaa ctaatccaat
     9241 ttctttattt taaataataa cgctccagtt tttctcttaa gtattcaaag gcttcttcag
     9301 gtttgtcagc ataagaaaaa agctgtaagt cctcaggact tatcatgtga tgcttggcca
     9361 tagcctcgaa atctataatc tcatcccaat attttttacc gtaaataagt atagctaatg
     9421 gtttttctat ctttttggtt tgaactaaag tcagtaattc gaataattcg tctaaagtac
     9481 cataaccacc gggaaaaacg acaagcgctt tagcgaggta agcaaaccag aattttctca
     9541 taaaaaaata atgaaactca aaatttaact ctggcgaaat atagggattg ggaatttgtt
     9601 caaagggcaa gcttatgttg agcccgatcg tcgggcctcc ggcctttttg gctccgcgat
     9661 tcgccgcctc cataatgcct gggccgccgc cgctgcatac gataaagcgt ttacctccgt
     9721 ccagtttgga tgaccacctt gtcagcattt cagcaagctt catagcatca cggtaatatg
     9781 cggacatttc taaatctgac tgaactcgtt cgatttggcg tcgtaacgat accgacggtt
     9841 ttaattgatt attcgcttct ttctgtaaaa ccagcaattt gcgtttggca tctttgggat
     9901 caagaatgcg agcggatccg taaaatacga tcgtatcacg tatcccatac ttcttgaagc
     9961 gcgcatacgg ttcatgcatt tcactaataa cacgcaaagt tcgaccgtca gggctatgca
    10021 aaaaagaacc attgtcatat gcttttagag gcacacctgt ttcatccaaa tgcggcaacc
    10081 gagatttggg cataatacgt tccttaggat aacttataaa taggacggtt ataacttaat
    10141 acacacgaaa gtcaaactcc aaattttctt ttcgtgccgt cggatgttga gcgccacgcg
    10201 tcaaagtacg gacactttgc atcacgtaat ccctcaattc ggtcacggtt atttttccgt
    10261 tcttatcccg atccgcggcc attgtacgca gaccttctaa aaggccataa gtaaatactc
    10321 cgttttttat cgtcttacca ccgatatcgc cttcagccgc ccactccgta cccgatgcag
    10381 ccgaaatcac aacagcgcca gaacctcgct gtaaatccga aaaaagatgc tgcattaatt
    10441 caaaagaatt aaccaaacct acaccggatt ttgatttggt tttaacgttt ttaaatgcgc
    10501 gcacttgttg tgctttggta tctttggcgt tatctacgat ttctgtttca tcccgatcac
    10561 gttcacccga atgacatgca tctacaagca tcaatttttt acgagcagca atgccatcga
    10621 gcaagccttc catctcagcg aatgtgattc ccttttcgga agctttgtaa aagtcaatat
    10681 cgtatgtagc gaagacccaa tctaccgtgc tatccggtat tccatgacct gcaaaataca
    10741 ttacaacgcc atcctgaggt ttacaatttg ctataaaatt tcgcacttgg atgatgctgt
    10801 cttttgtggc ttgttcgtta agtagcgtct tgatgtgtaa ttttgaatat ggcaatttat
    10861 ttgaagctaa tgcgtttcga aaaaaatctg ttatgtcccc cgcatcttta gccgcaaatt
    10921 gtaagtcaaa ttcacgatcg gtatagtgtg atactcccac tgcaataacg tacaatgcag
    10981 gatctgtgtc ctgtgatcta cagtgcacat agcgcgtctc ttttaggctt tcgatgccgg
    11041 cggtattgat cacggacacc tgtattttgt tacgcccctg agtaagaggt atccgctgtg
    11101 tctcgttcca ttggttaaga taaccgtcac cgattttttt cccataagat gaatgcaaag
    11161 gaacgtcatt cacatttacc attagacgtt caagtggcgc ttctttgctg taggcggaaa
    11221 tcgtgagtat tacttcgcgt aaatccgttt ccgttacatc cccttttatc tgaatatcag
    11281 gaacctgaag agaagagccg agttggcttt cggtaagccc taaccgttta aggcgccgct
    11341 gatacgcacc atacagtgct gcgattcgag ctgaatcggc tacaccaagt ctttgtaata
    11401 cgatatccgg acgattaagc ctcagatcaa attgctcgaa tgggtagaat tttggcgtca
    11461 tatttagacc tttattgatc tgccagccca ctctttgtgc tccgcgtttg gatgcagcat
    11521 agtatccatc cgttgtccag attatccatt cgttatcgga agttacatac agcgaagcga
    11581 tatatgtcat tttgttttca tcatatattt tgatttcacc gctctcaaga ccgacataaa
    11641 accggcgccc atccgggctt tcagccagtc ctatcgtgcg cgcaccgtca tagctaagca
    11701 tattggccgg tgaatcaatg aaaattccat aatacgtacc caaaagtagt tttccgttct
    11761 gcagcttcat ggcacacagt ggacgatcgt ccagcaacca tttgatatcg ggaagcgata
    11821 ccggcgctct caattgcgca agccttgcgt tttcaaataa gctctgttca acactatccg
    11881 ttttaaattc aaacgtagag agattaaaag ttttagaaat tgtaccatag tcattcaatt
    11941 tttcataaaa gttaaatgtt tcaccgatcc cgatgacatt gggatgatca cgatgaatac
    12001 ctatagcgct tatctttcct gacttcgata catccccaag cgtcattatt ctttccccat
    12061 cggcgacatt ccaaacatgg atttcgttat gcaatccacc ggcggacact acgtgtttcc
    12121 catccgggct aaatgctaca gcaaacacgg gacggtcatg ctgtcgaaat gaaataacat
    12181 gttttttaga taatctgccg tccgcctctg aggtaagtga ataaacgcgc acttcattac
    12241 ccacacagac agcgagttgt tctcccgctg tatcaaacac catcgcagtg atcatatccg
    12301 atttgaaggt catgatatta gcaacaatgt tatcgccctc gttttgcagt gcatcaataa
    12361 tacgcacgcg ccctctaaaa tctccgatag cggcaagttt tcctttagca tcggtagcaa
    12421 tacttcggca cggcccatct acggctttcc cgatgacacg taaattttct ccagcttttt
    12481 ttctgaaaac caacccatga taatccgctg ccaaccatgc atttccgtta tccacaaaac
    12541 ctgttcctac cacctgatgg tcgcagatgc ctagaaagcc ccaattacaa aggccggctt
    12601 ttacggcttc ctgcgtagtt acggccgaat cacggagcac aaggtgtccc gtgttagtat
    12661 tcacatcata ttggttaatg cgattatcaa acgaaccgcc caccgaaaca accttttttc
    12721 cgtccggaga aaaacgaacc ccataaatag attgtttatg atgtgcagcg acatcaacga
    12781 cagcaccgct cacataatca agcatcagca cataatcgcc gatgtaattg gccgtcaaag
    12841 tattatatct ttgacctacg gaagagcctg caatgaggat gtacttttta tccggagaaa
    12901 tgtccatggt aaatatctgc ccttccgtac cggcatatcc tggaacgcgc aagcttcgta
    12961 aaagctgccc tgtccatgca tcccaaattc gtatggtttt atcccatgaa gctgtaacaa
    13021 tctctttacc atccgggcta tagcgtacag cattaacacg atcaagatgc aatccgcttt
    13081 caaaggttag caccggtggt tctgataatt cttggaacgg cgaaaaagtg gattgatttt
    13141 gagcacatat atatgtgcct aaaaatgcat acaataaaat cattattata tttttcatat
    13201 cttactcaat aaaaaaagcg gctatcgcaa tcagcaacaa ccgctttcct aggttggaca
    13261 caggttctta tttagaataa taattaataa tctgatctat ctgaggatac tgtttgggca
    13321 tttccgggtc gagttgcttg gcgcgctgca tacagtacaa ggcatcactt cctttgccta
    13381 aggcatagta tgaatatccc agaacaaaat aagcgacggc gttttcactc atctcatttg
    13441 atttattgct gagatcaatt gcattgttgt aattttgagc gctcatttcc tcccaagcct
    13501 tattccaata aaaatcggac atgagacctt tgagtctgtc atgatcggga aataatttat
    13561 atcctgaatc taatatttta tgagcttctg cagggttttc gctatttatt atttgatata
    13621 gattgctata tacgccgcca gtaatcggct tagtatcgat cgatttctgg taattgagct
    13681 tggcattggc tacatcgctt ttatagtgat aggccattcc aagtttataa tacgcccatg
    13741 ccattccggc tttgtgcccg atgcgggagt acattgccaa tgcattttga ctttcctcaa
    13801 cggctttatc atacattttt aattgataca tcaaatccag cgttttaatt tttaaatcgg
    13861 cagaatcggc tacgatttgg ttatacttaa aattaggtgc cgcacctacg gggtttttgc
    13921 aaaacttttg aatgtctgtc agattaggat acagcgctaa attttcttca gcttcgttaa
    13981 gttctaatag tttttgtacg gcaatgccgg ccttggtata ctgctttgtc tggtaaagtg
    14041 catagagtaa atatccatac gcttgagcgt ttttatcatc gtacttcacc agtttatcaa
    14101 gattcgttgc agcggcccaa tagttctttt tatcaaatgc gtctttgccg tcggtaaaat
    14161 atatattaca taagtatgtt ttgatctcat ccgatccgga caaattttta gataatcccg
    14221 ataaacacag tttctccgct tcgactacgt tcttatctat ttggtacagt atctgagcca
    14281 taaatttata agcaagtcct tcggcttcag gtgttgctat agatttttga aaccatgttt
    14341 ttgcggcagg atattcacct aatttgaatt tacaaatccc agcttccgta ctaacagatg
    14401 aaatgattcc ggtatttcct gtgttttgaa cacgagttat aatggtttca aattttttaa
    14461 aggcatcctc aaagttgtta ttttccttat cttgcaacgc caagcgatac attttaaaag
    14521 cttctatgtc atcagaaacc agttgatacg tcggaagaaa ttgttttatg tatgctttgc
    14581 gatcattcat actcgtatct ttgacagcta aaaaagcacg accggcttcc acgacagccg
    14641 acgccacatc cttcggcatt tggtcaaatt tgtatttgga acgcgcttct tcgcgtataa
    14701 aagtaaatcg cgcaaacagt gtcatcggat atttattgtc aatggattcg gcttgctgaa
    14761 gatattcctt cgccacttca aacttgccaa attcagcata catcatagca acttctgcat
    14821 aggctcgcgg gtgctgggga tatttatcaa tggcatgatt ataatatgtc aaagccatca
    14881 tctcactttt catttcgact cctgcacgac catactcaca aagcgaatca gccggcgcat
    14941 tatacatcgg cattccaggc gctattttca attgcgcttg aagcgtaaca ctcaaaaaca
    15001 aagcaatgaa taaacttcta aacatacctc tccttaataa catttaatga gaaccaaaaa
    15061 aatattggat acaatttatt aattgtagta gccaataaaa tgccaaatat catggattat
    15121 aaattaagaa gatagatatg cagtattttt taatatgtcg cagatttgcg aatgaacggt
    15181 tatcaatgcg tcgcaattta acgactattg atatcagcta tcaaatattg gccacttctt
    15241 cttctaaaag ttggcgctca tgcatcgcgg cataggtgcc attttgctcc actagatcat
    15301 cgtgcgtacc cgtttcaatg atcgcgcctt ctttcaaaac gatgattaaa tcggcatgct
    15361 ttaccgtttg aatacgatgg ctgacaagta aagttgttcg atttttcatc agaatcttca
    15421 aacgcttcaa tatctcatct tctgtgtgtg tatcaacgct cgaaagcgca tcgtctagta
    15481 tgagtatctt aggttcgcga attacggcac gtgctatcga aaggcgttgt ttttgccctc
    15541 cggatagagt gatacctcgt tcgccgatga ttgtttcata tttttccgga aaatcctcaa
    15601 tattgcttcg cagttgtgct gtatcaacgg cccaaatgac tttatccgtc ggcggattat
    15661 caacgccaaa aactatgttt tgataaattg tatcggaaaa cagaaaatta tcttgagtaa
    15721 ccatgccgat gtgttgacgc aaaatagaca ccggaattgt attgatatca cgatcgtcaa
    15781 tgaatagcat tccgtccgga atatcataca aacggggaat cagattgacc aatgtactct
    15841 ttccggaacc tgtcgcaccg acgattccca aggtcattcc gggatggatg caaattgaaa
    15901 tatttttcaa aacaggtata tcgcgatatg aaaaattaag attacgaaat tctatcttac
    15961 ctgcaatgtc agagtgtccg ttactttgat tgtttttatc actgattgcg ggctgttcat
    16021 caaagatggc attcattcgc ttcatggaag ccgacccgcg ttgaaaaata ttaatcaccc
    16081 atccgacggc taccattggc cagatcagca tcgcgagata aaaattaaaa gccgtaaatt
    16141 caccaatgga cattacaccg tctatcacta aacttccgcc gtaccacagc aaaaccgcaa
    16201 cactcagccc tatggtcaaa atcacactgg acatcatgat cccgcgaatt cgaaccatgg
    16261 acatattgag ctggatatac tcatgactga gattttcaaa cttcttaatc tccgcggctt
    16321 cttggacaaa tgattttact acacggattc cggagaagtt ttcctgtgca gccgaactta
    16381 tatcggaaag tttttcctga actttttgcg agcgcgtcat gatcacacca gatagacgcc
    16441 ctaccgcaaa cgaaagtaag aataaaggca ggaaagccag taacgtaagc atcggactaa
    16501 tatgtatcat catcggtacg acgtatataa acgctacaat cgtattggcg gtgtacataa
    16561 tgccgggccc tagcacttct cgtacattgg ccagatcgtt ggtcagacgg ctcatcaggt
    16621 cgcccgtttt ggcatactga aaaaactgaa cgtgtaattt ttgcaaatgc gaaaaaaaat
    16681 cactgcgcag gtcatactcg atctttcgtg acatgacgat gagcgtttgt cgcatcagat
    16741 aaagaaaaat gccgtgtaac aattcgacca aaaggtacgc cgcaatgtat gccacaacga
    16801 tggccacacc aagttgtaac tcgacggaat ggatgatttg attgtataga ggaaatgttg
    16861 aaggatccgt gccgggcgct aatgtatcaa tagcttcacg taccaactga gggcctgtag
    16921 actgaaataa attttttaaa ataaccagca caaatccgct aatccaataa acgcggtatt
    16981 tccaaaggta cggcttcagg cgcttaaaca tagaatctta ttctttggta taaaggttgt
    17041 tttttttgat atacgataat actgcattcg gtacgatata gcgaaccgat tgatttcgta
    17101 aaagtcgttc acgcacccat gaagaagcaa tctcaatcac aggtttatca atacatatcg
    17161 cttcttttgt gaattgcaat tcgcctttat cgaagtcaaa acccatacgc ggatatacga
    17221 cgattttaga aagttcagtc aacctctccg gtgttttcca attaggtaga tcaagtaacg
    17281 aatccgatcc gatcatcaga aaatattcgt ttttaggatt ctcttttcgt aaaaactcca
    17341 acgtatgaac cgtatacgag agaccggata gtttttcttc aatgtcagaa cattggaaat
    17401 actcattttg ttcaatagct aaatccacca tcgttttgcg atgatgaaac gagattacgt
    17461 tttcagaagt tttatgcggt ggccgtgctg aggggataaa taccactttg gacaaaccga
    17521 gatgatcgcg aataatctca gcgatgatca gatgacctgc gtggatgggg ttaaatgttc
    17581 ccccaaataa accgatcttt tccaaatggt gtatttaatt tgccgatttg acagaagtgg
    17641 actgttcagg cttcactaat ttagcctctt tggccgcttt agcttgggcc ttgagttcat
    17701 ccttctttat atcttccagt gtttcgaggg catccgttac ccactctttt tctaacgggt
    17761 attcatcgat gaattttttt aataaaatct gagctttttg aaattcctcg attttatagt
    17821 tgcagtatcc tttccaatac ttggcttgca tagcatacgg cgagtcataa taatcggtaa
    17881 taacctgatc ataataaaca atggcggctt caaactcact gattttacgg taaatctgtg
    17941 cgctttcaaa atgtttacgt gctaatttgt cttttaccga tttataatgt ttatccatct
    18001 cagtgcgcag ttcgctgcgt ggaaacaaat caataaaatt ctgaatggca ttttgcgcct
    18061 gataggtgaa cttctgatcc aaggcaaatc ccggcgagag ttcgatatag cacaacgcca
    18121 actgatacat acttttttca ataaattcgc ttttgggata acttgatacc atacgttcaa
    18181 attctgaaga agccgaaacc caatcatgct gacgataata gctttcagcc aaataaaact
    18241 gcgcatcgtc ggcgatgtcc gaaccgctgt atttataggt tatcaaagaa aactgttcct
    18301 gtgccgaagt ccaatttttt ttattaaaaa acttcattgc gtattcaaaa tgttcccgtg
    18361 atgtcatgta ctgtgtaaca tcttttttgc tgcatccggt agactgtaaa aatgaacaag
    18421 ccatcgctaa aacaaaaatc tttctcaaca tgagatatcc tttacaaaat ggtatatatc
    18481 gttatgaata aaatttcaaa aaatcaaata tcatataacg catagtcacc gttgcgcgaa
    18541 acaacgatca tagtttcttc tttcattagc gtccatccgt cattgttgtc taaggcctcc
    18601 gaagtgacca gtgtgccgcc ttgatatgaa gtataataac ccaaactatg cattacttta
    18661 gttgcgatca ggtagtcccg cgtaagcatc ataaaattta attgcggttt tcgcgatgcg
    18721 tccgcacggc gactgatttc tctaagatcg gctattgtat gctgcaatga tgtacgtacg
    18781 aaatggatgt ccagtgtttg cgctggatta tatttacgaa gatacgtcag aaaaagatgc
    18841 atggcatatt ctgaatccgt attccccgta atatgtccgc taagcgcggg atcaattttg
    18901 gctaatgccg aatccataac gttttcaatt ccgtaaaccg acccgttgtg agcaaaaata
    18961 atgtcctgat gatatatcca gggatgtgta ttgaccttgg atgcaccgcc taccgatgcc
    19021 cggcgtatat gcaacatgac acgtttgaac ggccgatggg ctgtatccaa aaaaagttta
    19081 tcgttacggg gtgccgaagc ttcctttata acgtggaatt gtccattttc ttcgaaacca
    19141 aatccccatc cgtcgtcgtt gggtttacaa ctgtcacgat cgcatacgct ttgccggaga
    19201 attccgttag gtgcatcaag aattacgttt tgtaatgata ccggattcgg cccggcatac
    19261 cccatgagtc tgcacatcag tgttttatca attcaaaaca atgtttaaga tagactttaa
    19321 atttttcaga gttagccaaa cgatcatatt caattttcaa aatcgttgcc ggttttttat
    19381 tttgatcaag ttgatactgc attcgtttaa aggaatctaa atattttcct tgcaaagttt
    19441 caataaacgg gcggaaccta tcagcgtttt tggaatcacc cgataactgt tgtacgaccc
    19501 cctcaa
//
LOCUS       DAOQNQ010000031        19430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_984_length_19430_cov_5.412490, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000031 DAOQNQ010000000
VERSION     DAOQNQ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19430
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_984_length_19430_cov_5.412490"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="PK754_03660"
     CDS             complement(<1..1034)
                     /locus_tag="PK754_03660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNB08629.1"
                     /translation="MSDIQRDVQEMDVVFVGAGPSNLAAAYHLSRLIKEHNEAVATGK
                     KQGQKLEIMAAIVEKGKNVGDHMLSGAILDPSAIRELMPDFKEKGFPIESEVSGESVY
                     LLSESGKIKFPIIPPTLQNHGTYIVSLNKIADWLKTQIESEFGDSVFILTEQTGREML
                     YDGDKVIGVRLGDKNVDHHGKPKSNFEPGADIHAKVTVLGEGPRGSLTKQLVPRLKLN
                     EDRNPQVYGTGVKEIWELPAGRLKKGMVIHTAGWPLPSNHYGGSWLYMMSDTTCSIGF
                     VSALDYSNPSFDPHGAFTKFKTHPYIAGLLEGGKMVSYGAKTVSEGGYWSMPKLYADG
                     VVLVGESGGFL"
     gene            complement(1062..2846)
                     /locus_tag="PK754_03665"
     CDS             complement(1062..2846)
                     /locus_tag="PK754_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08630.1"
                     /translation="MRLRYYLITFTMISAAAWAQHKTMPETLFEGRYVMVLLNNRDSA
                     QFIKAQEMVNDNDGHIIHAFLPTIIQGYLTRRGEEVLCQSGLVREIVDSVFTGDMTRM
                     SERDRMIVRVWNQQFKPLLATRGQEEDGDNDRTPREVPGKYQTSEYMMGSVAVGVVFV
                     ESDGSLEKSTENWDDYSKEDMIFQIRKGLDWWAQQGGYRASLTWNYEFKDVTTKYEPI
                     THAYNDDENWVSDAAGKLGYGTGDGRMLCYEYANDLRLRYKTDWAFIVFAVAADNDDD
                     GNWKNSEAVAWANLGGPLLVINNRCDGWGPERAWEVMAHETGHIFNALDEYPGGSRGN
                     SRTGLLNVINGNAADGGIINEECIMKAHGDRACDFTRGQIGWIDENRDGVFVSDYLNI
                     SSRFNKKMMLAEAKKITPSDLKIDFEDPRVRMEMERNKKFYSDDFKTNTRGWNEDGAS
                     YIRNGEYSITNTTNWLQDEYSDVEVRVRVRWESGSVSDAFGMDMYSRTTNQFYTCVIN
                     QRGEVMAMQIKAGEGYYPEWKKVASLQKTGINELAMRCEKNTITMYVNGAQVASYTDD
                     GVDQRGVGLRADEGVRAAFDDLEIKKIK"
     gene            2986..3822
                     /locus_tag="PK754_03670"
     CDS             2986..3822
                     /locus_tag="PK754_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016761899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNB08631.1"
                     /translation="MNNMSLNIHSTLRLYNDVNIPCLGLGVWRTGPGDETRKAVDFAL
                     RSGYRHIDTAHFYKNEKDVGVALKQSGLDRKNVFITTKLWNDDHGYDRALRAFHESLS
                     LLNLTYVDLYLIHYPVSGKRSESWKALEKLYEEGLCRAIGVSNYTQRHLDELLQSARI
                     KPMVNQVEFHPWLYQNELLNFCKTNGIVLEAYSPLTKGQKINAPELSKIAAVYQRSPV
                     QILIRWALQHGIVVLPKSAQPARITENASVFDFNITDQDMARLDAFNCNLRVAWDPTN
                     TP"
     gene            3832..4290
                     /locus_tag="PK754_03675"
     CDS             3832..4290
                     /locus_tag="PK754_03675"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HNB08632.1"
                     /translation="MHKLIMLVLLLFVPLRDHRSEYEALHWLLDAWQYTKGSVTFIEN
                     WSHAAPQLFTAHNVTLKNNDTITYEDVRLEIVNDTLCYHVKPKGQALTAFMLVQLTDN
                     KAVFENFTNDFPQRITYHHTSADSLHAYIEGPSRGAWRQIDYYYARITKN"
     gene            4306..4662
                     /locus_tag="PK754_03680"
     CDS             4306..4662
                     /locus_tag="PK754_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HNB08633.1"
                     /translation="MKRIFYLASCSTCQKILKTVKPGKDVVLQNIKEETITPDQLDAM
                     KKLAGSYEALFSKRSMKFRAWNLHEKELTEKEMRDLILKEYTFLKRPVAVINDQIFVG
                     NTKIAVDGLKHALKEK"
     gene            4665..4961
                     /locus_tag="PK754_03685"
     CDS             4665..4961
                     /locus_tag="PK754_03685"
                     /inference="COORDINATES: protein motif:HMM:NF040323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HNB08634.1"
                     /translation="MLKQAIPLLHVSDVRQAEAFYCGVLGFEMYSSTRAREDMENPCY
                     MVVWRDNVVLHLSSFSGDGKSGGVVNILVENVDRLYDEFLQKNVSLDLQPTDQT"
     gene            complement(5031..5600)
                     /locus_tag="PK754_03690"
     CDS             complement(5031..5600)
                     /locus_tag="PK754_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08635.1"
                     /translation="MKKFIKENWGRFVFELITIIAGVLIALAVNEVRQNYAQQELADT
                     ALKSIVAEIESNKKFIEQRLPYHRRMVHILDSLALKNADRPFREVGRLAEWRGIQPPI
                     LSDAAWQTAIQSGALAKSDFEKATQIAKLYSFQNAYIRWGDNYIAAVIGGDLASVEQY
                     KNLFYEMGTTGGELVLFYASILNAIQTNK"
     gene            5755..7083
                     /locus_tag="PK754_03695"
     CDS             5755..7083
                     /locus_tag="PK754_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HNB08636.1"
                     /translation="MARIRTLIMGAAGRDFHNFNVIYRDNKAYEVIAFTANQIPNIDG
                     RKYPSALAGKLYPKGIPIFPQKDLDALIRKYRIDEVVFSYSDVSYNYLMSCSSIANAA
                     GAHFKILGADPTMIRSKRPVISICAVRTGSGKSQTTRRVSSILKSFGLKVAAIRHPMP
                     YGDLAKQRVQRFAQLADLKKHKCTIEEIEEYEPHIVTGTIIYAGVDYEAILRQAEKEA
                     DVILWDGGNNDMPFYKPDLHIVVADPLRAGHELLYYPSEANLRMADVVIINKEDSADM
                     KSTNLLRDHIHQVNAKAVIVDAASPIFVEHPEKIIGKRVLVVEDGPTLTHGEMKFGAG
                     TVAAQKYGARMIVDPRPYTSGSITETYKKYPDIGVLLPAMGYGSKQVKDLETTINRTP
                     CDTVIIGTPIDLHRIINIKKPSVRVRYDLQEIGTPNLDTLLQKFVKRMKL"
     gene            7156..7680
                     /gene="mog"
                     /locus_tag="PK754_03700"
     CDS             7156..7680
                     /gene="mog"
                     /locus_tag="PK754_03700"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013459875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HNB08637.1"
                     /translation="MIKIGILTASDRASAGVYEDLSGKAIMETLRVYLRSEWMPVYKV
                     IPDDRTSIEKELADMADREACCLIVTTGGTGPAPRDVTPEATEAVCDKMMPGFGELMR
                     AVSLQYVPTAILSRQTAGIRGKTLIVNLPGKPKSIRECLDAVFPAVPYCIELIGGPKL
                     ECHANAVKAFRPKE"
     gene            complement(7702..8946)
                     /locus_tag="PK754_03705"
     CDS             complement(7702..8946)
                     /locus_tag="PK754_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08638.1"
                     /translation="MRKILILVYSFVFTAYVYTQEEADARAIGMALAQTAVSENTDAY
                     RWNPANLGLNYKLKPKLTIAYTGFGFNNRNNIFSRTDYNKYNGRVLSEADKADIRDLL
                     KGKKAFDFNGSGAARALSVQYKNYAVNFEAGGNGNMQLPREILDVVFAKDAYEVGSEN
                     LSARGKGGGMAALRTSLSAGFPLTGLFDKHIREIAVGATLTYMRGIKQVTIKDFRAFI
                     AESDSFITGARFHTLQSSGGNGFSLSFGVSAQINKEWFAGISVIDVYSKMQWKTLNEK
                     RVAQFDVRSEDIFNAKKKSVDTTIYGGQYSTRLPGMLRAGVGYQLDKEWLLAGDIEVI
                     LHNAPGNVTPRIAVGAENHRWRKVILRSGLSVGGVNQGLDMALGAGFLMGKTILDVGT
                     NNLEGMISQRSFSFALQIKTVF"
     gene            complement(8943..11051)
                     /locus_tag="PK754_03710"
     CDS             complement(8943..11051)
                     /locus_tag="PK754_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08639.1"
                     /translation="MPKTFYTYTLMWVSLVSGFTFFSACEKPVEPQFTSALRFPLSKD
                     IYDIFDLKKDIENGKDSLAVDIISDSVVIRIFDRETIHAGDELNVDPTSDSADAAINN
                     DLKVRDSVDINLRLGDLAPGFWSGLHGTNAVIPAFNMPGSYTPTSRNTTQFTQFKSAT
                     LYSGNVALRFTNNTSCAMDSLNVVIFNSTSRTRIDSFVIKNIPATLGSVSQKSITYPA
                     PSSKVVRDSVTIRIAGYSSGSGSSELVDSTAALAIHIVSDITASSVTGKFPSQTISNT
                     DSIVSDSQSDIDSAYIERGSIRLNIRPLGLSVNASVTFTMYDFVDNNGVPLTRSFNII
                     AGQNSTQILDLKNYRLIPNWSVSSSRFVDYRYTIVTVASTNNNTVISNGNGVRVVAVV
                     DSLHFSRMRGTLSNEDFTISNRSQDVNIRDLDSIQLRSAFFEIITSHRIPFPVAMSMT
                     LTGKRGTRSASTVINGSLNKYTSGTDPQRDTLRTPLAEYDEVAALLNLVPDSIYVSGG
                     AIVGDNVANGVITKQDSIDVRINLRAPLVFKLPNDSTKNRIRPTPHVFDVGEGTRKRI
                     YSNLESVRITGTIKNHFPVPVSVQFIIDSTADAARFYDSTNAYKLVYPSSPLIIGAGQ
                     VNSSTGLVTAPTTTTLDYALNKTEFQRLFAPEITKRFRGIKAKLMQTPGTDFVKVSSA
                     DYVDLDTEIEIEVQIDENLQ"
     gene            complement(11094..11477)
                     /locus_tag="PK754_03715"
     CDS             complement(11094..11477)
                     /locus_tag="PK754_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB08640.1"
                     /translation="MSGKPKSILYIEDNELNRLLVRRLLEHAGFEVFEAGDGAKGVKA
                     AEELKPDLILMDINLPIMDGFEATRQIKSNPELKHIPIIAITSNAMQGDRERTLAAGC
                     DAYIRKPIDPDAFPGQIAHFIEVFS"
     gene            complement(11481..13115)
                     /locus_tag="PK754_03720"
     CDS             complement(11481..13115)
                     /locus_tag="PK754_03720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB08641.1"
                     /translation="MTHIPHSGDITIIEYDLTPKAASRKKPAPTGDVSIEGLINENVM
                     AEDVRSETEFKKKKKTRKKSSAKNADDTNAGIELEKIVYSSEHLTTIVDHLTDLATEI
                     EHQRLPDTRSPEVTTRCFQLRSCPDHDCVAYQASHARCWYIAGEMCGCRTHLGLPSCF
                     ECQVFQAAATEPLARMRETYLYVLGMLRRRNEKLRTMQRESDLLKERINQVRRIQSSE
                     QLSELDEVFFEQRLVDELVKFSRRTDEFETERDKKLFEQHKILTEQLGSAYLQLQSVT
                     SELERSNKQLEDKVTERTDDLRKSNMALREAVKHSQEADRLKSEFIANVSHELRTPLN
                     SIIGFSKLIMAGVDGPITERQGTDLNAIYNSGKHLLEIINSILELSKIEAGRTDLDIK
                     SFTLEPMVDEIIAATQSLLVGRPLKLEKNIESSLPEIEADMTKIRQVIYNLVSNAVKF
                     TEEGAILISAARDGDWVIVRITDTGIGIDKEHIQSIFERFRQVDGSTTRKAGGTGLGL
                     NIAKKFIELHRGQMHVESRPGEGSTFSFKIPIKHTI"
     gene            complement(13118..14050)
                     /locus_tag="PK754_03725"
     CDS             complement(13118..14050)
                     /locus_tag="PK754_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNB08642.1"
                     /translation="MSRTTGKWFCVINPAANRGNGTRCVPEVRRFLSERNIPHEILVT
                     TQSSEAMPYVVERAADHDVIIAAGGDGTVHEVVNGLMQAYAQSGGQPPKAALGVLPIG
                     SGNDFAKMLHMPKELRPALDAIATGSRRLIDIGRISVDGDHARYFDNNVGIGFDAYVN
                     YESIQIKSLRGVLIYLAAVFKSLFKYQHPMAEIRINGETINNKILMMTTGNGSCSGGG
                     FYITPHAKIDDGWLDVCIVDSRSKLKMLLDLPTVMQGKHDGAPGVRMYRTKNVRIASS
                     ELLPIHADGEVVSMDAHTVELEVMPTAIHAIFAH"
     gene            complement(14097..14483)
                     /locus_tag="PK754_03730"
     CDS             complement(14097..14483)
                     /locus_tag="PK754_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015146676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNB08643.1"
                     /translation="MPKEFVRTDLAPAPIGPYNQAVIASGKMIFTSGQIALDPKTGQL
                     LIGDVRQQTRLVLENLSAILRAGGSDLNKIIKTSVFLKDMNDFAAMNEVYAEYMSEAA
                     PARSTVEVARLPKDVLVEIDCIALVE"
     gene            complement(14500..14958)
                     /locus_tag="PK754_03735"
     CDS             complement(14500..14958)
                     /locus_tag="PK754_03735"
                     /inference="COORDINATES: protein motif:HMM:NF027592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HNB08644.1"
                     /translation="MKHFLSIVMAMTWLGCSKPAPAPQMDSFGKGVTMSEVTPIAQAV
                     AQVGRYKDKEILIESEIKSVCQKKGCWMEIATENQPMRITFENYGFFVPKDAAGRKVK
                     VQGKIEEKEIPQDEARHYLEDAGKSKEEIEKIVGPQKVVTMVASGVAIVK"
     gene            complement(15001..15201)
                     /locus_tag="PK754_03740"
     CDS             complement(15001..15201)
                     /locus_tag="PK754_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08645.1"
                     /translation="MFGLIRLLLVGAVAYAAFRVIRKLWIVTDPKNNPMRSSSSGDKN
                     KEVRTTVFKSEDIQDAKFKDIK"
     gene            complement(15213..16220)
                     /gene="pheS"
                     /locus_tag="PK754_03745"
     CDS             complement(15213..16220)
                     /gene="pheS"
                     /locus_tag="PK754_03745"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNB08646.1"
                     /translation="MDFEQIKSQFQSDTDGQILDKAALEQLQTKYLGKKGILTLATEA
                     LKALPKEQKPQFGKMLNEVKQSVEARLAEIKAAVQAAEVDPKDDFDATLPGRHGWVGH
                     NHPLTIVLNDIKQIFYEMGFTIEDGPEVELDYYNFETVNIPKDHPARDMQDTFFINEN
                     VVLRTHTTPVQARTMQRMKPPIRMICPGRVYRKDTPDATHLPFFNQIEGLVVDEHVTF
                     ADLKGTLAAFVHRLFGNDIGVRFRPSFFSFVEPGAEVDINCIFCRGKGCRTCKNSGWM
                     EILGAGMVHPQLFEYAGYEKNKYTGYAFGMGLDRIVMPLYGINDLRLLIENDVRFLEQ
                     F"
     gene            complement(16362..16715)
                     /gene="rplT"
                     /locus_tag="PK754_03750"
     CDS             complement(16362..16715)
                     /gene="rplT"
                     /locus_tag="PK754_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNB08647.1"
                     /translation="MPRSTNNPASKARRKKIFKQAKGAWGKRKNVLRSAIETVEKGMT
                     YATRDRKANKRNFRSLWIIRINAAVREHGMTYSKFVAALKKANVDVNRKMLAELAMND
                     AAAFNKLIEVAKAAN"
     gene            complement(16758..16955)
                     /gene="rpmI"
                     /locus_tag="PK754_03755"
     CDS             complement(16758..16955)
                     /gene="rpmI"
                     /locus_tag="PK754_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNB08648.1"
                     /translation="MPKTKTNSGAKKRFHVTGSGRILRKKATHRHNMSAKSNTRKSNQ
                     VGKTEVADSDNHRISHLLGLK"
     gene            complement(16989..17507)
                     /gene="infC"
                     /locus_tag="PK754_03760"
     CDS             complement(16989..17507)
                     /gene="infC"
                     /locus_tag="PK754_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNB08649.1"
                     /translation="MGKEDKIRINEQIRTPKVRLIGPKGEQIGIVDIKEALRRSQEEG
                     QDLLEIVPTADPPVCKILDFGKYRYEIQKREKEARKKQTHTGEVKELRFRPVTDDHDY
                     QFKVKHAVNFLKEGYKVKMVVVYKGREIANKEAGIQLAERMVTDLESVGKLDGEMKFE
                     GRNMVMIFIKNR"
     gene            complement(17536..>19430)
                     /gene="thrS"
                     /locus_tag="PK754_03765"
     CDS             complement(17536..>19430)
                     /gene="thrS"
                     /locus_tag="PK754_03765"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNB08650.1"
                     /translation="SFESGITPMAVATSISRGLAEKCVVAEVNGKQVDLSTPITGDAS
                     LKLFTSDTAEGMQVFWHSSAHVMAQAVKRLWPEAKFEDGPPTEAGFFYDILIPEKISE
                     ADFVRIEAEMEKIAKEKLTYTRAELTRGEALEFFKKIGQDFKLDIIERIPEGAPISSY
                     TQGEFTDLCRGPHIPHTGMIKSFKIMSVAGAFYKGDENNVQLQRLRAISFPSKKQLDE
                     YLTMLEEAKKRDHRKLGRELELFMITPMIGGGLPVWLPNGTLLRRTLENFLKDELLRR
                     GYQEVVTPHIGNLNLYRTSGHYPYYADSQFSPIKVEDEEYILKPMNCPHHHQIYASKP
                     RSYRDLPLRLAEFGTVYRYEQSGELSGLSRVRGFTQDDAHIYCTHDQLKNELINAIDL
                     TQLVFKTFGMPVKTRLSFRDPKTPDKYAGSPEMWDQAEREIHEVADAMKLDYFVGIGD
                     AAFYGPKFDFIVRDALGRQWQLGTVQVDYVMPERFGLEFIGGDGQKHRPVIIHRAPFG
                     SMERFISILIEHFAGNFPVWIAPVQIAVLPISDAHTSYAEQVKIALEKEKIRCQLDTR
                     NEKIGYKIREAEMKKIPYILVVGEKEMQTQTVAVRRHGKGDQGAAGLAEFIAQVKQQI
                     EDKILD"
BASE COUNT         5071 a         4727 c         4211 g         5421 t
ORIGIN      
        1 agaaacccgc cggattcgcc gaccaatact acgccgtcgg catacagttt gggcatggac
       61 caatagccgc cttcagaaac cgttttagcc ccgtaggaaa ccattttacc gccttcgagc
      121 aatccggcaa tgtaaggatg ggttttaaat ttggtaaacg cgccatgcgg atcaaacgat
      181 ggattgctgt aatccaatgc cgaaacgaat cctatcgagc acgtcgtatc gctcatcata
      241 tagagccaac ttccgccgta atgattactc ggaagcggcc atcccgctgt gtgaataacc
      301 atacctttct tgagacgacc ggcaggcaat tcccatattt ctttcactcc ggtaccgtac
      361 acttgcggat tgcgatcttc attgagtttg aggcgcggta cgagttgttt ggtcaaactt
      421 ccgcgcgggc cctcaccgag caccgttact ttggcatgaa tatctgcgcc gggttcaaaa
      481 ttggatttcg gtttgccgtg atgatccacg tttttatcgc cgagccgcac accgatcact
      541 ttgtcaccgt cgtacaacat ttctcgaccg gtttgttcgg taagaataaa tacgctatcg
      601 ccaaactcgc tttcgatctg cgttttgagc caatcggcaa ttttattaag cgaaacaatg
      661 tatgttccgt gattttggag cgtcggaggt atgattggaa atttaatttt tccggattct
      721 gataataaat aaaccgattc accactgact tcggattcga tcggaaatcc tttttcctta
      781 aaatccggca tcagttcgcg tatcgccgac ggatcaagga tcgcaccgga taacatatga
      841 tcgccgacgt tttttccttt ttcgacaata gccgccatga tttccaattt ctgtccctgt
      901 ttttttccgg tcgcaacggc ttcgttatgt tctttgatga gacgggagag atgatacgcc
      961 gcggccaaat tagacggacc ggcgccgaca aatactacat ccatttcctg cacatcgcgt
     1021 tgaatatcag acatactcta atctcctcat atcgtatggg actatttaat ttttttgatt
     1081 tccagatcgt caaatgctgc gcgaacacct tcgtctgcgc gcaaaccgac gccgcgctga
     1141 tccacaccgt catccgtgta gctggcaacc tgcgcgccat tgacgtacat cgttatcgtg
     1201 tttttttcgc atcgcatagc aagttcgttg ataccggttt tctgaagcga ggctactttt
     1261 ttccattcgg gataatatcc ttcgccggct ttgatctgca tcgccatgac ttccccgcgt
     1321 tgattgatca cgcaggtata aaactgattg gtcgtacggc tgtacatatc catgccaaat
     1381 gcgtccgaca cggaaccgct ctcccaccgc acgcgtacgc gtacttcgac gtcactgtat
     1441 tcgtcttgca accaattggt ggtattggtg atggagtact ccccgtttcg gatatacgac
     1501 gcaccgtctt cattccaccc gcgcgtatta gttttaaaat catcgctata aaattttttg
     1561 ttgcgctcca tctccatacg aacgcgcgga tcttcgaaat caattttcag atcggaaggc
     1621 gtgatttttt tggcttccgc gagcatcatt tttttattaa accgtgacga gatattgagg
     1681 taatcgctga caaaaacacc gtctctgttt tcatcaatcc atccgatctg gccacgtgta
     1741 aaatcgcacg cgcgatcccc atgcgctttc attatacatt cttcattgat gatgccgccg
     1801 tcggcggcgt taccgttaat aacattgagc aaacctgtac gcgaattgcc gcgtgatccg
     1861 ccggggtatt cgtccaacgc attaaaaata tgcccggttt catgcgccat cacttcccat
     1921 gcgcgttccg gtccccatcc gtcgcaacgg ttgttgatca ccagcaaagg cccgcccaga
     1981 ttagcccagg cgacggcttc gctgtttttc caattaccgt catcatcatt atcggccgct
     2041 acggcaaata cgataaacgc ccagtccgtc ttgtaacgaa ggcgcaaatc attggcgtat
     2101 tcataacaga gcatacgccc gtcaccggta ccgtaaccca atttgcccgc cgcgtcactc
     2161 acccaatttt cgtcatcgtt atatgcgtga gtaatcggtt cgtatttcgt cgttacgtct
     2221 ttaaattcat aattccatgt cagactcgca cggtagccgc cttgttgagc ccaccagtct
     2281 agccctttac ggatttgaaa tatcatatct tctttggaat aatcatccca gttttccgta
     2341 cttttttcca gcgatccgtc gctttctaca aaaacaacac cgacggccac ggatcccatc
     2401 atgtattcac tggtttgata ttttccgggc acctcgcgtg gcgtgcgatc attgtcaccg
     2461 tcctcttcct gccctcgtgt cgccaagagc ggtttgaatt gttgattcca aacgcgcacg
     2521 atcatccgat cgcgttccga catacgcgtc atatcgccgg tgaataccga gtccacgatt
     2581 tcgcgtacca atccgctttg gcaaagcact tcttcaccgc gtcgtgtcag ataaccttgg
     2641 ataatggttg gcaaaaacgc gtgtatgata tgaccgtcat tatcatttac catttcctgc
     2701 gctttgatga attgcgcgga atcccgatta ttgagcaaca ccatgacata tcggccttca
     2761 aaaagtgtct cgggcatagt tttgtgttgc gcccaagctg cggccgaaat catcgtaaaa
     2821 gtgatcaaat agtaacgtaa cctcataaaa aacctcatat tgtgatgttc ccaaaagacg
     2881 ccggaaatta ttatctttca ccgccaaagg caaagtaaat tttggtattg gaatgcacca
     2941 tggccggcat tagattgata cattacacac tcattttgta aacctttgaa caacatgtcg
     3001 ctgaatatcc attctactct acgactgtac aacgatgtaa acataccctg tctcggttta
     3061 ggtgtctggc gaaccggccc gggtgatgaa acacgaaagg ccgtagattt tgctttgcgc
     3121 agcggttacc ggcacattga tacggctcat ttttacaaaa acgaaaaaga cgtcggcgtt
     3181 gctttgaaac aatcagggct tgatcgcaag aatgtcttta tcacgacaaa actgtggaat
     3241 gatgatcacg ggtacgatcg tgctcttcgt gcgttccatg aaagcctatc tttgctcaat
     3301 ctgacctatg ttgatttata tctcattcac tatccggtga gcggcaaacg cagtgaaagc
     3361 tggaaggctt tggaaaaatt atacgaagaa ggtctgtgcc gcgccatcgg cgtaagcaac
     3421 tatacgcagc gtcatttaga tgaacttctg caatccgccc gcatcaaacc gatggtcaat
     3481 caggtagaat ttcatccctg gctttatcaa aatgaactgc ttaacttttg taaaacgaac
     3541 ggtatcgtac tcgaagcgta tagtcccttg accaaaggtc aaaaaatcaa tgctccggaa
     3601 ctgtctaaaa tcgccgcggt atatcaacgc tcacccgtgc aaatattgat tcggtgggcg
     3661 ttacagcacg gtatcgttgt attgcctaaa tcagcgcaac cggcgcgcat tactgaaaat
     3721 gcatccgtat ttgattttaa tattaccgat caagacatgg cacgactgga tgcattcaac
     3781 tgcaatttgc gtgtggcttg ggacccgaca aacaccccct aaggagaaaa tatgcacaag
     3841 ttaatcatgt tggttttact acttttcgtt cccttacgcg accaccgttc agaatacgaa
     3901 gcattgcatt ggctgctcga cgcgtggcaa tacaccaaag gatcggtgac atttatcgaa
     3961 aactggtcac acgccgcacc ccagcttttt acggcacaca atgttacgct gaaaaacaac
     4021 gatacgataa cgtacgaaga tgtacgcctc gaaatcgtca acgatacctt atgttatcat
     4081 gtcaaaccca aaggtcaggc attaacggct tttatgctcg tacagttaac agacaacaaa
     4141 gccgtttttg aaaatttcac caatgacttc ccgcaacgca tcacctatca tcatacatcg
     4201 gccgattcac tgcatgccta tatcgaaggc ccttcccgcg gcgcttggag acaaatagac
     4261 tattactacg cacgcattac caaaaactaa aataacaatc acatcatgaa acgtattttt
     4321 tatttggcat cgtgcagcac ttgccaaaaa attctcaaaa cggtcaaacc cggtaaagat
     4381 gtagtactac aaaatataaa agaagaaacc atcacgccgg atcaattgga tgccatgaaa
     4441 aaactggccg gttcctacga agcattattc agcaaacgtt ccatgaaatt cagagcatgg
     4501 aatttgcatg aaaaagaact gacggaaaaa gaaatgcgcg acctgattct caaagaatac
     4561 acgtttctca aacgcccggt tgccgttata aacgatcaga tatttgtagg taatacaaag
     4621 atagccgttg acggtttgaa gcatgcattg aaagaaaaat aaatatgttg aaacaagcta
     4681 tacctctgct gcatgtaagc gacgtacgac aggcggaagc gttttattgc ggcgtgcttg
     4741 gctttgaaat gtactcttca acccgtgcaa gagaggatat ggaaaacccc tgttacatgg
     4801 tcgtttggcg tgataacgta gtcttgcatc tgtcgtcttt ttcgggagac gggaagtccg
     4861 gtggtgtggt gaatatctta gtcgaaaacg tagatcgttt gtacgatgag tttttacaaa
     4921 aaaacgtgtc cctcgattta caaccgacgg atcagacatg aggtacgcgt gagatgtatg
     4981 taaaagacac cgaccaaaat tgcctgcgtt tttttacatt gaaataacga tcatttattt
     5041 gtttgtatgg catttaaaat agacgcataa aaaagtacaa gctccccgcc ggtagtcccc
     5101 atttcataaa aaagattttt atactgttcc acggacgcga gatcacctcc gataactgcg
     5161 gctatataat tatcacccca gcggatatac gcattctgaa acgagtataa ttttgcaatt
     5221 tgcgtcgctt tctcaaaatc ggatttagcc aaagcgccgg actgaatcgc tgtttgccat
     5281 gcggcatcgc tgagtatcgg tggttgtata ccgcgccact ccgccaaacg tcctacttca
     5341 cgaaacgggc gatcggcatt ttttaatgct aatgaatcca atatatgcac catgcgacga
     5401 tgatagggca atcgctgttc gataaacttt ttattcgatt cgatttctgc gacgatactt
     5461 ttcaacgctg tatcggccaa ttcttgttgg gcataatttt gccttacctc atttacggct
     5521 aatgcaatga gaacccccgc aatgatcgta attaattcaa aaacaaagcg cccccaattt
     5581 tctttgataa atttcttcat tcgatatcct aagcatattt atttaagata gactgccaaa
     5641 tataatgaat caagcattta gggattgttt actcatatct ctgcatgact gagatcatac
     5701 ccgtgtgcat tttcctattg tacgttataa cgttgtaaac tcggaggttc ttttatggca
     5761 cgtattcgaa ctttaatcat gggcgcagcc ggccgtgatt ttcataattt taatgtaatc
     5821 tatcgcgata acaaagcata cgaagtcatt gcttttacgg cgaatcaaat acccaatatt
     5881 gacggacgca aatatccgtc ggcattggcg ggaaaactct atccgaaagg tattccgatt
     5941 tttccgcaaa aagatttgga tgcattgata cgcaaatacc ggattgacga agtcgttttt
     6001 tcctacagtg atgtctctta caattatctg atgtcatgca gctcgattgc caatgcggcg
     6061 ggcgcgcatt tcaaaatcct cggcgctgat cccacgatga ttcgcagtaa gcgtccggtg
     6121 atttccattt gcgctgttcg taccggttct ggaaaaagtc aaaccacgcg gcgtgtttct
     6181 tcgattctca aatcgttcgg gcttaaggta gcggccatcc gtcaccctat gccttacggt
     6241 gatttggcca aacaacgtgt acagcgtttt gcgcagcttg cggatctcaa aaaacataag
     6301 tgtacaatcg aagaaattga ggaatatgaa ccgcacatcg taacgggcac gatcatttat
     6361 gccggcgtgg attatgaagc gattttacgt caggccgaaa aagaagcgga tgtgattttg
     6421 tgggacggcg gtaataacga catgccgttt tataaacccg atctgcatat cgtggtagcc
     6481 gatccgctgc gtgcagggca tgaattgttg tattatccca gcgaagccaa tctccgtatg
     6541 gccgatgttg taatcatcaa taaagaagat tcggcggata tgaaatccac caatctgctg
     6601 cgtgaccata ttcatcaagt caatgccaaa gccgtgatcg tggatgccgc ttcgccgata
     6661 tttgtcgaac atcctgagaa gatcatcgga aaacgcgtgc tggtcgttga agacggccca
     6721 acgctcacgc atggtgaaat gaaatttggc gccgggacgg tggccgcaca aaaatacggc
     6781 gcacgaatga tcgtggatcc gcgcccttac acatccggga gtatcaccga aacttacaaa
     6841 aaatatcccg acatcggcgt cctgttgccc gcgatgggct acggtagcaa acaagtcaaa
     6901 gaccttgaaa cgacgatcaa ccgtaccccg tgcgatacag tgatcatcgg cacgccgatt
     6961 gatttacatc gcattattaa tatcaaaaag ccttccgtgc gtgtgcgata cgacctacaa
     7021 gaaatcggca cgcccaatct cgatacattg ttacagaaat tcgtgaaacg catgaaatta
     7081 taacgtttca tttttttcta cccttccaat cccgtcatga ttaccttcct gacgggattt
     7141 tttttgggag ataatatgat caaaataggt attctcactg cttccgatcg tgccagcgcc
     7201 ggtgtgtatg aagatctttc cggtaaagcg attatggaaa cattgcgcgt gtatctgcga
     7261 tccgagtgga tgccggttta taaagttata ccggacgaca gaacaagtat cgaaaaagaa
     7321 ttggccgata tggcggatcg tgaggcatgc tgtctgatcg taacgaccgg cggaacgggt
     7381 cctgcaccgc gcgatgtcac gccggaagct acggaggcgg tgtgcgataa gatgatgccg
     7441 ggatttggtg aattgatgcg tgctgtatcg ctgcagtatg tcccaacggc cattctttca
     7501 cgccaaacgg ccggcattcg cggtaagacg ttgatcgtca accttcccgg aaaacccaaa
     7561 tccattcgtg aatgtttaga tgccgtattt cctgctgtgc cttattgtat tgaattaatc
     7621 gggggaccaa agttggaatg tcatgccaat gccgtaaaag cttttcggcc aaaagaatga
     7681 ctccgtaaga attttgcgat tttaaaatac ggtttttatt tgcagggcaa aagagaaact
     7741 gcgttgagaa atcatgcctt caagattatt cgtgcccaca tcaagaattg tttttcccat
     7801 caaaaaaccg gcaccgagtg ccatatctaa accttggttg acaccaccga cactgaggcc
     7861 actgcgaaga ataaccttgc gccagcggtg gttttcagct ccgactgcaa tacgaggcgt
     7921 cacatttccc ggcgcattat ggagtatcac ctcaatatcg ccggctaaaa gccattcttt
     7981 atccagttgg tagccgactc cggcgcgaag catccccggc aaacgggtag aatattgccc
     8041 tccgtaaata gtcgtatcta cggatttttt cttggcatta aaaatatcct cgctgcgtac
     8101 atcaaactgg gccacacgtt tttcattcag cgttttccac tgcatcttgc tatatacatc
     8161 tattacgctg attccggcaa accattcttt attgatctga gcgctgactc caaaactaag
     8221 ggaaaaacca ttaccgccgg aggactggag tgtgtgaaat cttgctccgg taataaaact
     8281 atccgactct gcaataaagg cgcgaaaatc tttgattgtg acttgtttta ttccgcgcat
     8341 atacgtaagc gtggcgccga cggctatttc gcgaatgtgc ttatcaaata aaccggttag
     8401 cggaaaaccc gccgaaagcg aagtacgcaa cgcggccatg ccgccgccct tgccacgcgc
     8461 gctaagattt tcggaaccga cttcgtaggc atctttagca aaaactacat ctaaaatctc
     8521 acgcggcaat tgcatatttc cgttgccacc cgcttcaaaa tttacggcat aatttttata
     8581 ctgcacactg agtgctcgcg ccgcgccgga accgttaaaa tcaaacgctt ttttaccttt
     8641 caaaagatca cgaatatccg ctttgtcggc ttccgaaaga acgcgcccgt tgtatttatt
     8701 ataatctgtg cgtgaaaaaa tattatttcg attgttaaag ccaaatccgg tgtatgctat
     8761 cgtaagtttc ggtttgagtt tgtaattcaa accaaggttt gccggattcc atcggtaagc
     8821 atccgtattt tcggaaacgg ctgtctgagc gagcgccatc cctatcgcac gtgcatccgc
     8881 ctcttcctgc gtataaacat acgcagtgaa aacaaagcta tatactaaaa ttaatatttt
     8941 tctcattgta aattttcatc gatttgaact tcaatttcga tctccgtatc caaatccaca
     9001 taatcggccg aactgacttt tacaaaatct gtcccgggcg tttgcatgag tttggctttg
     9061 ataccccgga aacgttttgt tatctccggc gcgaataatc gttgaaattc tgttttattg
     9121 agggcgtaat caagcgtcgt ggttgtcggt gcggtaacca atccggttga cgaattaacc
     9181 tggccggcac cgataatcaa tggcgaagat ggatatacca gcttataagc attggtcgaa
     9241 tcataaaaac gagctgcatc ggcggtgcta tcaataataa attgaaccga cactggaacg
     9301 gggaaatgat ttttaatagt accggttata cgaacggatt ccagattgga ataaattctc
     9361 ttgcgggtac cttcgccgac atcaaacaca tgcggtgtag ggcgtatgcg gtttttggtg
     9421 gagtcgttgg gcagtttaaa aacaagcgga gccctcagat tgattcgcac gtctatggag
     9481 tcttgtttgg taataactcc gttggccaca ttgtcgccga ctatcgcgcc accgctgaca
     9541 taaatcgaat ccggaacaag attaagaagc gcagccactt catcatattc ggctaacggt
     9601 gtacgcaagg tatcacgttg cggatcagtt cccgatgtgt atttattaag actgccattg
     9661 atcaccgtcg aagccgaacg cgttccgcgt ttgccggtca acgtcattga catagcgacc
     9721 gggaaaggaa tacgatgcga agtgatgatt tcaaaaaaag cagaacgtaa ctgtatgctg
     9781 tctaaatcac gaatattgac atcctgactg cgattggaaa tagtaaaatc ttcattagat
     9841 aaggtgccgc gcatgcgaga aaaatgtaat gaatcaacca cggcaacgac acgtacaccg
     9901 ttcccgttgg agataactgt attattatta gtagaagcaa cggtaacaat cgtgtatcta
     9961 taatccacga aacgactgct actaacggac caattaggaa taagccgata atttttcagg
    10021 tcaagtatct gcgtactatt ttgcccagca atgatattaa aacttctcgt taacggtaca
    10081 ccgttgttat ctacaaaatc atacattgta aaagtaacgg acgcattgac ggacaacccc
    10141 agtggacgaa tattgagtcg aatgctgcct ctttctatgt atgccgaatc tatatccgac
    10201 tggctatcgg aaacaataga atccgtatta ctgatagttt gcgacggaaa tttaccggta
    10261 accgatgagg ccgtgatatc agaaacaatg tgaatggcta acgcagcggt cgaatcaacc
    10321 agttcagagc ttcccgaacc actggaataa ccggctatac gaatcgtcac cgaatcccgt
    10381 acaactttgg atgaaggcgc aggatacgtg atactttttt gggatacgct gcctaaagta
    10441 gccggaatat ttttgatgac aaaactgtcg atacgtgtac gcgatgtcga attaaatatt
    10501 accacgttca gcgagtccat tgcgcaactt gtattattgg taaatcgtaa agctacgttt
    10561 ccgctataaa gcgtcgctga tttaaactga gtaaactgcg ttgtattgcg cgatgtagga
    10621 gtgtaactgc ccggcatatt gaaagccgga ataactgcat tcgtaccatg cagacctgac
    10681 caaaaacccg gtgccaaatc gcccaagcgc aaattgatat caacggaatc gcgaaccttg
    10741 agatcattat tgatcgccgc gtcagccgaa tccgatgtag gatccacatt caattcgtct
    10801 ccggcatgta tggtttctct gtcaaaaata cgaatcacta ctgaatcgct gataatatcc
    10861 acggccagcg aatcttttcc attttcgata tcttttttca aatcaaaaat atcgtagatg
    10921 tctttgctca gaggaaaacg taacgcggaa gtaaattgag gctcaacggg tttttcgcat
    10981 gcagagaaaa aggtaaaccc cgacacaagg ctcacccaca tcaacgtgta ggtataaaat
    11041 gtttttggca tattttttaa atataaaaaa aaggcgcacg tgcgcaagag caatcaggaa
    11101 aaaacttcga taaaatgcgc aatttgcccg ggaaaagcgt ccggatcgat cggtttacga
    11161 atgtacgcgt cacaacctgc agccaatgtg cgttcacgat cgccttgcat ggcgttggac
    11221 gtaatagcta tgataggaat atgttttaat tcaggattag atttaatttg gcgtgtggct
    11281 tcaaatccgt ccatgatcgg caaattaata tccatcaaaa tcagatcggg tttcaattcc
    11341 tccgctgctt tgacaccttt ggccccatca ccggcttcga aaacttcaaa ccctgcatgt
    11401 tcgagtaacc tgcgcaccag taaacgattg agttcgttgt cttcgatgta taaaatgctt
    11461 ttgggtttac cgctcatatg tcagattgta tgttttatcg gtattttaaa cgaaaacgta
    11521 ctgccttcgc ccggacgact ttcgacatgc atctgaccgc gatgcaattc gataaatttt
    11581 ttagcaatat tcagccccaa acccgttccg cccgctttgc gcgtggttga accgtctacc
    11641 tgacgaaatc gctcgaaaat agattggata tgttctttat caatgccgat gccggtatct
    11701 gtgatgcgta caatgaccca gtcgccatcg cgagccgcgc tgatcagtat ggccccctct
    11761 tcagtaaatt taaccgcatt agaaaccaga ttatagatca cctgacgtat cttcgtcata
    11821 tcggcttcga tttccggcaa cgacgattca atatttttct caagtttcag cggacgcccg
    11881 accagcagtg attgcgtagc ggcaataatt tcatccacca taggttccag cgtaaacgac
    11941 ttgatatcca gatccgtacg accggcctcg attttggaaa gctcaaggat gctattgata
    12001 atctccagta aatgtttacc gctgttataa atagcattca aatcggtacc ctggcgttcc
    12061 gttatcggcc cgtcaacacc ggccatgatt aacttggaaa aaccgataat cgaattaaga
    12121 ggtgtacgca attcgtggct cacattagca ataaattccg atttcaaacg atcggcttcc
    12181 tgcgaatgtt tgacggcttc gcgcaaagcc atgttgctct tacggagatc gtccgtacgc
    12241 tccgttactt tatcttccaa ttgtttattg gatcgctcca gctcggacgt cacagattgc
    12301 aattgcagat aggcgctgcc taattgttcc gtgagtattt tatgctgttc aaatagtttt
    12361 ttatcccgtt ccgtttcaaa ctcgtccgtg cgccgcgaga atttaaccaa ctcatcaacc
    12421 agtctttgtt caaaaaacac ctcatccaac tccgacagtt gttcactgct ttgaatacgc
    12481 cgaacctgat tgatgcgttc tttgagcaag tccgactcgc gctgcatggt gcgtaatttt
    12541 tcattgcggc gacgtaacat gccgagcaca tacagataag tttcacgcat ccttgccagc
    12601 ggttcggttg cggccgcctg aaaaacctgg cattcgaagc atgacggcaa tcccagatga
    12661 gtgcggcaac cgcacatttc acccgcgata taccagcagc gggcatgcga agcctgatag
    12721 gctacgcaat catggtccgg gcatgaacgc agttgaaagc aacgggttgt tacctccggt
    12781 gagcgtgtat ccggtaaacg ttgatgttcg atctccgttg cgaggtcagt caagtgatcg
    12841 acgatcgtcg tcagatgttc gctggaataa acaatttttt caagttcgat gccggcatta
    12901 gtatcatctg catttttagc cgaacttttt tttcgcgtct tttttttctt tttaaattca
    12961 gtttcgctgc gtacgtcttc cgccattacg ttttcattga tcaatccttc gatggataca
    13021 tcaccggttg gcgcaggctt tttacgtgaa gctgctttag gcgtcagatc gtattcgatg
    13081 atggtaatat cgccgctgtg cggaatgtgt gtcataatca gtgcgcaaat attgcgtgaa
    13141 tagccgtcgg cattacttct aattcgacgg tgtgggcgtc catagatacg acttccccat
    13201 cggcatggat cggcagtaac tcagacgacg caatgcgaac atttttggta cggtacatac
    13261 gaacgcccgg cgccccatca tgtttacctt gcatgactgt gggcaagtcc aacagcattt
    13321 ttaatttgct gcgggaatcc acaatacata catccaacca tccgtcgtca attttggcat
    13381 ggggtgtgat ataaaatccg ccgccggagc atgaaccgtt gcccgtcgtc atcattagaa
    13441 ttttgttgtt tatcgtttcg ccattgattc gtatttccgc catcggatgc tggtatttaa
    13501 aaagcgattt gaatacggcc gcaagataaa tcaaaacgcc gcgcagcgat ttgatctgaa
    13561 tgctttcata attgacatat gcatcaaagc cgatgccgac attattgtcg aaataccgtg
    13621 catgatcgcc gtccacgctg atacgaccta tatcgatcaa gcgccgcgat cccgttgcga
    13681 tcgcgtccaa cgccggacga agttctttag gcatatgaag cattttcgca aaatcattac
    13741 cggatccgat cggaagtacg cccagtgcgg ctttgggagg ctgaccgccc gactgcgcat
    13801 aagcctgcat caatccgttg acaacttcgt gtaccgtacc atccccgccc gcggcaatga
    13861 tcacatcgtg atccgctgcg cgctcgacca catacggcat cgcttcactg gattgggtgg
    13921 tcaccaatat ttcatgcggg atatttcgtt ccgacagaaa gcgccgcact tccggtacgc
    13981 atcgcgtacc gttgccgcgg ttagccgccg gattgatgac gcaaaaccac ttgccggttg
    14041 tcctactcat acaccgtcac cgtttgttta gtgaaaacgt ctatttaaaa tagtgcttat
    14101 tcgacgagcg ctatacaatc aatttccacc agcacatctt tgggcaggcg tgcgacttct
    14161 acggtggatc gtgccggtgc ggcttccgac atatattctg catatacttc attcattgcc
    14221 gcaaaatcat tcatatcttt gagaaatact gatgttttga tgattttatt cagatcgctt
    14281 cctccggcgc gaagtatcgc gctaaggttt tcaagcacca ggcgtgtttg ctggcgtaca
    14341 tcgccgatta atagctgtcc ggttttggga tcaagagcaa tctgtccgga ggtgaatatc
    14401 attttaccgg aggcaatgac agcctgattg taaggcccaa taggagccgg cgccagatct
    14461 gttcttacaa attctttggg catattccct cgttaataat tattttacaa tcgctacacc
    14521 ggaggctacc atcgttacca ctttttgagg gccgacgatt ttttcaattt cttctttgga
    14581 tttacccgca tcctccagat aatggcgtgc ctcgtcttgc ggaatttctt tctcttcgat
    14641 tttgccctgt acttttactt tacgaccggc ggcatcttta ggaacaaaaa aaccatagtt
    14701 ttcaaatgtg atgcgcatcg gttggttttc cgttgcgatc tccatccagc agcctttttt
    14761 ctgacaaacc gatttgatct cggattcgat cagtatttct ttatctttgt agcggccaac
    14821 ttgtgccacc gcttgggcga tcggcgttac ttccgacatg gtcacacctt tgccaaaaga
    14881 atccatttgc ggtgccggtg caggtttgga acaacctaac caagtcatgg ccatcactat
    14941 gctgagaaaa tgcttcattt gttttccttc tttaaactat tatagagtta gaaattaatc
    15001 ttacttaatg tctttgaatt tagcgtcttg tatatcttca gacttgaaga ccgtcgttcg
    15061 cacttcttta tttttatcac ccgaagagga actgcgcatc ggattattct taggatccgt
    15121 gacaatccat aatttacgaa taacacgaaa ggccgcatac gcgacggcgc cgaccaataa
    15181 caatcgtatt aaaccaaaca taaaaatcct tattagaatt gttcgagaaa acgcacatcg
    15241 ttttctatca aaagtcttaa atcattgatt ccgtacaacg gcatgacgat acgatccaag
    15301 cccataccga aggcatagcc ggtatattta tttttttcat aaccggcgta ttcaaataat
    15361 tgcggatgca ccatgccggc gcccagaatt tccatccatc cggaattttt acaagtacgg
    15421 cagcctttac cgcggcaaaa tatacagttg atatccacct cggctccggg ttcaacgaac
    15481 gaaaaaaatg acggtcgaaa gcgaacgccg atatcattgc caaacaaacg atgaacaaac
    15541 gcggcaagcg ttcctttcaa gtcagcaaac gtgacatgtt catccactac cagcccttcg
    15601 atttgattaa aaaaaggaag atgcgtggcg tcgggcgtat cttttcggta aacgcggccg
    15661 ggacagatca ttcggatcgg cggcttcatc cgttgcatgg tgcgcgcctg aacaggtgtc
    15721 gtatgcgtac gcaaaaccac attttcgttg ataaaaaaag tatcctgcat atcgcgtgca
    15781 ggatggtctt tgggaatatt caccgtttca aaattgtagt aatccaattc gacttccgga
    15841 ccgtcttcga tggtaaatcc catctcgtaa aaaatctgct tgatgtcatt gagaacgatc
    15901 gtcaaaggat gattgtgccc tacccatccg tgacgccccg gtaatgtggc atcaaaatca
    15961 tccttcggat caacttccgc cgcttgcacg gccgctttga tttcggccaa ccgcgcctca
    16021 acactttgtt tgacttcgtt gagcatttta ccgaattggg gcttttgctc tttcggcagc
    16081 gccttgagcg cttcggtagc cagcgtcaga ataccttttt ttccaagata ttttgtttgc
    16141 agttgttcga gtgcggcttt atccaatatt tgcccgtcgg tatcgctttg aaactgcgat
    16201 tttatctgtt cgaaatccat gacttccttc ttcaaaaatt catcattcac atggtatcaa
    16261 aaaaaacacg tattcgcaag atttttgccg tcctgtgaag caaaaaaaag acatgcctca
    16321 tagtgtggca tgtcttttat acattcgtct gttatgcgaa attagttcgc tgctttagct
    16381 acttcaataa gtttattgaa tgccgctgca tcgttcatgg ccaattcggc cagcatttta
    16441 cgattcacgt caacattggc ttttttcaac gcggcaacga atttggaata cgtcatgccg
    16501 tgttcacgga cagccgcatt gatacgaata atccacaagc tgcggaaatt acgtttgttg
    16561 gctttacgat cgcgcgttgc ataggtcatt cctttttcga cggtttcgat cgcgctacgc
    16621 aatacatttt tgcgtttgcc ccaggcgcct ttggcctgct taaaaatctt tttccggcgg
    16681 gccttactgg ccgggttatt tgtggaacgt ggcatggtat tctcctttta aatttttttc
    16741 tttaattacg cctgcaatta cttcaagcca agcagatggc ttatgcgatg attatccgag
    16801 tccgccactt ccgttttgcc gacctgattg gatttgcggg tattggattt ggcgctcata
    16861 ttgtggcgat gggttgcttt cttacggaga atacggcctg agccggtcac atgaaagcgc
    16921 ttctttgcgc cgctgttggt tttagttttt ggcatgggat gctccttatt agattaattt
    16981 attttgtatt agcgattttt gataaagatc ataaccatat tacgaccttc aaatttcatt
    17041 tcaccgtcga gtttgccaac agactccaga tccgtcacca tacgctcggc caattgaata
    17101 ccggcctctt tgttggcaat ttcacggccc ttatacacaa cgaccatttt gaccttatat
    17161 ccctctttaa gaaaattgac agcgtgtttc accttaaact ggtagtcgtg atcgtccgtg
    17221 acaggtcgga aacgcaattc tttgacttcg cccgtatggg tttgtttttt acgcgcttct
    17281 ttttcacgct tttggatttc ataacgatat tttccaaaat ccaaaatttt acaaaccggc
    17341 ggatcagccg taggaacgat ttccaaaaga tcctggcctt cttcctgaga acggcgcagc
    17401 gcttctttga tgtccacgat accgatctgt tcacctttgg gtccgatcag tcgcactttg
    17461 ggtgtgcgaa tctgctcgtt gatacgaatt ttgtcttcct taccgatggc tggtctcctt
    17521 acttagtaaa cgtaattagt cgagtatttt gtcttcgatc tgttgtttga cctgcgctat
    17581 aaattccgcc aatccggcag caccctgatc gcctttgcca tgacggcgca ccgcaacggt
    17641 ttgtgtttgc atttcttttt caccgacaac aaggatgtat ggtatttttt tcatctccgc
    17701 ttcgcggatt ttataaccga ttttctcgtt acgcgtatct aattggcaac ggattttttc
    17761 tttttccaaa gcgattttga cctgttccgc ataggacgta tgcgcatcgg aaataggtag
    17821 cacagcaatc tgaaccggtg cgatccatac gggaaaatta ccggcaaaat gttcgatcag
    17881 aattgaaata aatcgctcca tcgaaccaaa cggtgcgcgg tgtataatta cggggcgatg
    17941 tttttgcccg tctccgccga taaactcaag tccaaaccgc tccggcatca cataatccac
    18001 ttgcacggtg ccaagttgcc attggcgccc gagcgcatcc cgcacgataa aatcaaattt
    18061 aggcccatag aatgctgcat cgccgatacc gacaaaataa tctaatttca tggcgtcggc
    18121 tacttcatgt atttcgcgct cggcttgatc ccacatttca ggggatcccg catatttatc
    18181 cggagttttg ggatcgcgga aagacagacg cgttttgacg ggcatgccaa atgtcttaaa
    18241 cacaagttgc gttaaatcaa tcgcgttgat caattcattt ttaagctgat catgcgtgca
    18301 ataaatatgc gcatcatctt gcgtaaatcc gcgtacacgc gaaagtccgc ttaattcacc
    18361 ggactgctca taccgataca ccgtgccaaa ttcagccaat cgcaacggaa gatcgcgata
    18421 acttctcggc ttggaagcat agatctgatg atgatgcgga caattcatcg gtttaagaat
    18481 atattcttcg tcttccactt tgatcggtga aaactgagaa tccgcataat agggataatg
    18541 cccggacgta cgatacaaat taagatttcc gatatgcggt gttaccactt cctgatatcc
    18601 gcgtcgaagc aattcgtctt tgagaaaatt ttccaacgtc ctgcgcagca aagtgccatt
    18661 ggggagccaa accggcaacc ctccgccgat catcggcgta atcataaaaa gctcaagttc
    18721 gcggcccagt ttgcggtgat cgcgtttttt ggcctcctca agcatcgtaa gatattcatc
    18781 aagctgcttc ttggacggaa agctaatagc acgtaaccgc tgaagttgaa cattattttc
    18841 atcaccttta taaaaggcgc cggctaccga catgatctta aaggatttga tcatccctgt
    18901 atgcgggata tgcgggccac ggcaaagatc agtaaattca ccttgcgtgt aggaactgat
    18961 cggagcgcct tccggaatac gttcgatgat atccagttta aaatcctgac cgatcttttt
    19021 gaaaaactcc agcgcttcgc ctcgtgtcag ttctgcacgt gtataagtaa gtttttcttt
    19081 ggcaattttt tccatctccg cttcgatgcg gacaaaatcg gcctcactga ttttttccgg
    19141 aatcaaaata tcatagaaaa aaccggcttc tgtcggggga ccgtcttcaa atttggcctc
    19201 cggccatagt cgtttgacgg cttgggccat gacgtgcgcc gacgaatgcc agaaaacctg
    19261 catgccttcg gcagtatccg aggtaaaaag tttgagcgaa gcatcgcccg tgatcggagt
    19321 ggacaaatcc acctgtttgc cattcacttc ggcaacgaca catttttccg ctaacccgcg
    19381 cgaaatggac gtcgcgacag ccatcggggt aatgccggat tcaaaggaac
//
LOCUS       DAOQNQ010000032        19402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_988_length_19402_cov_6.558950, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000032 DAOQNQ010000000
VERSION     DAOQNQ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19402
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_988_length_19402_cov_6.558950"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PK754_03770"
     CDS             complement(<1..636)
                     /locus_tag="PK754_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB08651.1"
                     /translation="MIYRITKDLFQMFMILSILTSCEEKKEFQNAAYLSISAFADSIK
                     KNPDALLVDVRTYKEYMSGHIPGAQNVNWANVGTFDYHAAQWDIGKPIFLYCSVGGRS
                     TGARRKLRNMGFRRVYELDGGFVNWRAKGFSETTLNERGGMSRSYFDSLITSDRPVLI
                     DFYTTHCSPCRLMEPIVNEVAEKSDIIVVSINIDDHTLIANDLKIHTVPTFH"
     assembly_gap    915..1014
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1099..1443
                     /locus_tag="PK754_03775"
     CDS             1099..1443
                     /locus_tag="PK754_03775"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB08652.1"
                     /translation="MEKEGTRGVAELAESACMCERHLRRKTKEKFGLPPHRLLENIRL
                     AKAIEAIHEQPEVALFQISQIAGYASYKSFYQAFTRRLKVTPSEAKQHLKQNPSVTMY
                     ALREKIEKKLTI"
     gene            1622..2941
                     /locus_tag="PK754_03780"
     CDS             1622..2941
                     /locus_tag="PK754_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08653.1"
                     /translation="MRIFSLLITLVFLLMNCKVIAQNQTLEQSVMVDMINPRSLDHPI
                     YLSFKDGDYLTSIIECDKWDSIYRGTNSEDAFMEQEAYYNSFVGNYQKALENFDKINA
                     KWINQSDTTNLLELSFLSAKKVDAVNEILREAVNHQITIVNEAHHMPQHRAFISSLLK
                     PLYESGYRYFATEMLTDNVDAVKKDGYVSFQQNGFYPNEPVYANLIRKALEIGYTLVS
                     YEDTVNCDFDPTNPLACFNQREQNQAKNISNLILKNYSNAKILIHCGYQHVDEIEHQS
                     PKVKTMAAHLKELSDSDPLTIDQTVMSEHGSVNFENFYYRYAQDSLKLKSPTVFKSNS
                     GYWVDDTKRGFVDMQVFHPRTNYINGRPNWLIQENHILTLPDFDISSFPFLVQAFKKS
                     DKTHSIPMDQFLVNSRKKIAVAVPKGYYRIRIINNKGTIHELEVKNP"
     gene            2993..3271
                     /locus_tag="PK754_03785"
     CDS             2993..3271
                     /locus_tag="PK754_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08654.1"
                     /translation="MIKMALICYSDSNFTGAANFFIKATKIDTTDGGLYYNVACCFSR
                     IGKIDKSFEWLNVAIQKGYNDYQWINNDPDLFNVRQDRRWEEFIKIKK"
     gene            3283..4095
                     /locus_tag="PK754_03790"
     CDS             3283..4095
                     /locus_tag="PK754_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08655.1"
                     /translation="MNINIALIGMIVLILLETGCSQTHSFMSEKIEACGLSDSLMIDE
                     KQLFKKIDSLIEFSRCDTSVIFGMNLFKKNEKKSDWIAIKLYARRPKQRVLDRLEMLK
                     KSDQLYRKKIIDSIGPSKGWASEEVRQLWIKQAEIDSLNWLTFNSKIIDHYGWPKISE
                     YGYEASATAFLIVQHTNAKNQEQKLPLLMKLADEGEFSMSEIALLIDRIKVSKGEFQV
                     YGSQVEFDAISKKFRFLPIEDEKNVDVRRTKVGLPPIQEYAKRWNIDYSYIK"
     gene            4448..6034
                     /locus_tag="PK754_03795"
     CDS             4448..6034
                     /locus_tag="PK754_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08656.1"
                     /translation="MKISIRFFGDYTFNTPSDWDSVKTTKLALQRINKINDFSLSESD
                     ILVSTRTVIEPYFSSIISLIPSSKIRFDHLKSFDSLGTDKQGHALYQNISPQDSFCLF
                     QKLIKLNNYWLHVTGQTINSTDGSCYSSSLVDEYNKISNSIEYIKRSERAALKNPYLI
                     ADSLFNSDSNGNYIKPIDYLKSEKNVYENSSLRNSYVQCLETYKSFVGMTEPSLVPIN
                     VYFPNPTNSFEPFSSEFGGIVDTIISLTSNNKIIIFNESHIYPSQRLLLSGLLNKLYE
                     AGFRKIALEAVWNDKEINAIGFPTLKSGFYTREPQMANLIRKAKEIGYQVISYEDPTL
                     GNREYNQAVTLSREFRKDSTAKMIVLCGSYHILEKRSDSSRTWMAEHLKEISKIDPLT
                     FNTVFFENVYFDNPRLLSLISGSKLNEIAPVGFNFVNDYYLFNVTSLKPFTCDNEKGN
                     FAPYKPIQIVVPQLIKKVLLVQIFSSKEYGIRGRVPVASHYVDSENCQMTYWLPPDEY
                     VVVYYGASGEIILVNKIILS"
     gene            6545..6715
                     /locus_tag="PK754_03800"
     CDS             6545..6715
                     /locus_tag="PK754_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08657.1"
                     /translation="MKTKEKMEVIDSPLFQTIEREQMKSVQGGKPPGWTMAEQTVYSN
                     GTSSEDSAAGND"
     gene            6800..7756
                     /gene="gwsG"
                     /locus_tag="PK754_03805"
     CDS             6800..7756
                     /gene="gwsG"
                     /locus_tag="PK754_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="grasp-with-spasm system ATP-grasp peptide
                     maturase"
                     /protein_id="HNB08658.1"
                     /translation="MVLILTYDGDTTSDYIIEVLKNKKHPYLRLNSSLFFKEKITIAP
                     LHKFIEINGFKYTSDLLRVIWFRRFYSYRNLKFIYPKRIVNKSDFPNLTFEFNKIIRT
                     LYATLNSSHWLLDPRHAFVNKIEVLQTAKEIGLLVPNTTIINNKAFLSKKENSNLVSK
                     SIAEGVGVKAKRYNFSMYTKTISPDFISKLPSLYVPSLVQERIKKRYDIRIFYLNGRI
                     FASAIFSQNNERTIDDFRIYDDNRPNRIVPYKLPNAIKRGIRRLMKKLSLNIGAIDMV
                     RSQTGEYYFLEVNPNGQFGMISHPCNYNLFEVVADYLIRNDK"
     gene            7854..9497
                     /locus_tag="PK754_03810"
     CDS             7854..9497
                     /locus_tag="PK754_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08659.1"
                     /translation="MGRVVYHNDSLRLSAMFYGDYVFHSGNKQREISRKSIEIIKSGY
                     KEGLYLTEKDLIFTAETIVEPKFYSALFSLHNLSKRRLNEISKIAPDFFWSDSVRNVA
                     GIGQIFTDATSAVFLIGFTYDHLTVRNKNSLQQQFFFNQTLNALSREFRDADESIFKS
                     VLSGSNYKLFEAEDPFILANKYYQTEYSGNYLLPINELLESEPNYVSTQDRYKYLQAL
                     LTYMSFIDHPQYDYYNKKRKQIQSRSIINYSKRFNLNIDEIQKTILDSSSHQRVVMFN
                     ESHYVPSIRSFIMTLLDGLYQQGFRYLALEALEEDRLVNEKKFPVISSGFYFRDFRMA
                     NIARMAKKLGYHLIKYDSRETNREEKQAQRLAQLIANSDTNKIVVLSGFSHIDENPDK
                     KRMASILKKISGINPFTINMTDISETRIELNSNICFTNNSTDRIQLPSYFMNNDLYVS
                     ICNDDFINDAFTFHKHETLNIHLDFSGIAKRGEYVLLVFDISESPNEGQLPVFAKLVL
                     PKMHEQIDIKLLPGRYNVLLKSEDGLICHDTTLYVEVNK"
     gene            10497..10862
                     /locus_tag="PK754_03815"
     CDS             10497..10862
                     /locus_tag="PK754_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08660.1"
                     /translation="MSKKFSLALVFYVCFATVKSQDIKNIDEQATALYLKKEYLKCAE
                     TFQKAIAADSNYFSAYYNTACCYTLAGKFELSIKFLKQAIDRGFSNERMLKTDPDLNQ
                     LRSRDEWKEILEYFHKVRY"
     gene            11077..12387
                     /locus_tag="PK754_03820"
     CDS             11077..12387
                     /locus_tag="PK754_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08661.1"
                     /translation="MKNMKSFAILILLSLNQIYAQYSLPPAIMDSIRDIDRSLSSDRN
                     YIAALTRLNRIHAMPQYAGQPILFNHLGLYNSLVSDYSTAVAFFDSIELLRQSQRHTT
                     SLPPTGLTFDTSQIRPFPAMETILRLAKNNSVVMINERHHMPMHRAFILELLIRLKAL
                     GYTHYAAEGLQNHDTITSPATYPAIGKTGYYVNESVFGELIRTAILLGYKIVAYETTS
                     SASPESDPIKNQNKREKEQAENLYNKTLRQDPHCKVLVHVGYGHNDKMGVGGWIPMAS
                     YFKTISGVEPLSIDQIEMFEHRVPYFENPYYRFISGMISQPSVFIENSSNTFWSYLQR
                     GSYDLEICHPKETYVHGRPTWRNIANRYRMYDLPISKKTIAKGLLQAFYHDEPSDAVP
                     ADQIVFNTGVQQMIPCLFLQPGKYRFKLVSEDSTISTEWVDIIK"
     gene            12390..14591
                     /locus_tag="PK754_03825"
     CDS             12390..14591
                     /locus_tag="PK754_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HNB08662.1"
                     /translation="MNSFPIYLQHDKMDCGPACIRMITKFFGRTVSPEKLRRITYASK
                     GGTSLLNLSEAAESLGFRTLAVKVSLEKLQKAPLPCIVFWQQRHFVVVYKIGRRYIHV
                     ADPAHGIVKYRHADFVSGWYSDRSNEGEGQGIALLFEPTPALYESEDDFEKSSKPNKK
                     GFGLLWPYLRSHRKLIFQLGLGFLLGSILQLIFPFLAQSMVDMGIHHQNIGFVYTILL
                     AQLMLFLSRITVESIRRWILLHISTRLQISLISDFLMKLMRLPVAFFDAKQTGDLMQR
                     IHDSHRIENFLTVTSLSFVFSIFNLFIFGAIILLYSPFIFLIFTTFSAVSITWITFFM
                     KKRRELDYKRFDRMSENQQGILQIIMGMQEIKLSGSEKQKRWKWESLQAKLFTISRKA
                     LGLGQVQETGTLFINELKNIMITFIAAKETIDGHITLGAMMAIQYMIGQLNAPIDQMI
                     LFLQHAQDAKLSLERLSEIHTHDDEDHESDNSAQLPQERSIRLDRVSFQYEGPRSPYA
                     LKDVNLPIHEGKITAIVGESGSGKTTLLKLLLKFYTPTEGKISVGSDALSQISASFWR
                     RQCGVVMQDGYIFSDTIAHNIAPADETVDYRRLIQALEAANIRTFIESLPLRYNTKIG
                     NEGLGLSQGQKQRILIARAVYKNPQYLFFDEATSALDATNERIIMYNLQRVFQNKTVV
                     IIAHRLSTVRNADQIIVLEKGNVVESGSHIELTQQRGVYFELVRNQLELGN"
     gene            14595..15875
                     /locus_tag="PK754_03830"
     CDS             14595..15875
                     /locus_tag="PK754_03830"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNB08663.1"
                     /translation="MSNDIEPLERIHSDSYDDVMNYEPHWLIRWGSFILLLIIIFLIT
                     VSGYIRYPDILRGQITLISDQPTLEVIAQSAGRVKLFVKDRDTVKVGQPLAMIDNPAD
                     FASIRKLRLFLDSASSQSDSYVMIDTALQLGELQAFYSEVIQNYKELVSFKALNYEVK
                     KINSIRQQINAYYTLGDRLEDQIKLLKLDVTKAEKDFEVNNSLHERGLLSEVELSGYQ
                     FQLNQKKIALEEAQKEAIRNSIVVSDNLKNILDMEFQNQDKTVRYLIRIQESMRKLRS
                     ELSAWEKKYWMTSPVNGEVIYNDVWTDDRFVKLGQEIMTINPKTNRIRGRVKYEGPGL
                     GKVVPGQEVKIKLDNYPFQEFGIVPGIVENISAVSKDKSVVIQITLPQQLKTSFGALL
                     EYKQEMQGTSEIITENRTLLERIFQQLRQITQYQ"
     gene            16217..>19402
                     /gene="nifJ"
                     /locus_tag="PK754_03835"
     CDS             16217..>19402
                     /gene="nifJ"
                     /locus_tag="PK754_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNB08664.1"
                     /translation="MSNTKRAMVTLDGNEAAAYVAHKLSEVIAIYPITPSSAMGEFSD
                     EWSSKGKANLWGTVPLVMEMQSEGGAAGTVHGALQTGSLTTTFTSSQGLLLMIPNMYK
                     IAGELTSTVFHVAARALASQALSIFGDHSDVMSVRATGWAQLSAHSVQEVMDMALIAH
                     AATLRARVPFIHFFDGFRTSHEVMKVEQLTDEDMLGMIDDHLIRAHRERGLSPEHPVM
                     RGTAQNPDVFFQGREAANPYYQKCPAIVQETMDRFAKLTGRAYKLFDYFGAPDAEHII
                     VAMGSGALTAQETAAFLIGQGHKVGVVKVHLYRPFSIEHLIAAFPKTVRTVAVLDRTK
                     EPGSAGEPLYQDVITALTETFIEGKLPFAMPRVIGGRYGLSSKEFTPAMVKAVYDELQ
                     KPLPKNHFTVGIHDDVTHLSLDVNPDFSIEPQDVVRALFYGLGADGTVGANKNSIKII
                     GEDTDNFAQGYFVYDSKKSGSITVSHLRFGPKPLFSSYLVTRANFIACHQFSFLEKYD
                     MLKYAVEGGTFLLNSPFDENEVWDQLPLIAQKEITEKKLRFFVIDGYRVAKETGMGGR
                     FNTIMQTCFFAISGILPRDKAIEAIKHAIQKTYGKKGDEIVRINFNAVDQTLTNLKEV
                     NYAGKPLTDRAMRPPVDPLAPDFVKDVTGRMILGDGDTLPVSAMPPDGTFPTNTARWE
                     KRNIALEIPVWDPAVCIQCNKCALVCPHATIRAKVFPETALDGKPEHFKAVPYKGKEF
                     PNSLYTIQVAPEDCTGCGLCVDVCPAKNKQETRLKAIYMQPQSPIRDQERIHWEYFLH
                     LPEADRTQVPLNTVKGSQLLQPLFEFSGACSGCGETPYLKLISQLFGDRAMVANATGC
                     SSIYGGNLPTTPWAQNKDGRGPAWSNSLFEDNAEFGLGFRLTVDKQTAFAQELLYRLG
                     DSLDKELIASLIASPQKTEADIQAQRTRVQVLKNRLTALSHPDAKRLLDVADFLVKKS
                     VWIVGGDGWAYDIGYGGLDHVLASGRNVNIIVLDTEVYSNTGGQASKSTNRGAVAKFA
                     ASGKRYSKKDLGLMAMSTGHVYVAQIAMGAND"
BASE COUNT         6151 a         3883 c         3746 g         5522 t
ORIGIN      
        1 gtgaaacgtc ggcacagtat ggattttcaa atcgttcgcg atcagcgtat gatcgtcaat
       61 atttatacta actacgatta tgtcggattt ttctgcaact tcatttacaa taggctccat
      121 caagcggcag ggcgagcagt gcgtcgtgta aaaatcaatt aacacgggtc tatctgaagt
      181 aatcaaggaa tcaaaataac tgcgcgacat accgccccgt tcatttaatg tggtttcaga
      241 aaatcccttc gcccgccaat tcacaaatcc gccgtctaat tcataaacac gacggaaacc
      301 catgttgcgc aatttgcgcc gagcgcctgt actgcgtcct ccgacggagc aatacaaaaa
      361 tatcggcttt cctatatccc attgcgcagc gtgatagtca aatgtcccga cattcgccca
      421 attgacgttt tgggctccgg gaatatggcc tgacatatat tccttgtacg ttcgtacatc
      481 aaccaaaaga gcatcaggat tttttttgat actatccgca aacgcagata tcgaaagata
      541 tgctgcgttt tgaaattcct ttttttcctc acacgatgtc agtatggaca atatcataaa
      601 catctgaaat aaatctttgg tgatacgata gatcatagat aaaagtttaa ttccgatttt
      661 atcatcatgt caatttatat ttgacgctca cagaacacaa gagaaaagac gacttcccaa
      721 ccaatctacc gcaatttttt taaaaaaaac tctcaaaatg aacacttaat acctacgcat
      781 tcatgtagtt tatttgtgtt agaattttga cacttaagtt tttcgggaat gtccgatttt
      841 aggtattgat ttgcctcact atgtgtatta ttattttact gtagcacaat tcaacaaatt
      901 tatctgttct cgtcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngaatgt
     1021 tctcgtcgaa tgttctcgtc gaatgttctc gtcgaatgtt ctcgtcgaat gttctcgtcg
     1081 aaacagagga ggaagggatt ggaaaaagag ggaactaggg gtgtcgcaga attggccgag
     1141 agcgcatgta tgtgcgaacg ccacctccgc cgaaaaacga aagaaaaatt cggcttacca
     1201 ccgcaccggc ttttagagaa tatacgattg gccaaagcaa tcgaagccat acatgaacaa
     1261 cctgaagtcg cgctgtttca aatcagtcaa attgccggat atgcatcata caaatcattt
     1321 taccaggctt ttacgaggag attaaaagtg acgccgtcgg aagcaaaaca gcatttaaaa
     1381 caaaatccaa gcgttacgat gtatgctcta cgagagaaaa tcgaaaaaaa actgaccata
     1441 taatgagcag tatacggatt ttagtttaaa gcaattaacg tgttttgttg attactctca
     1501 aatatatcat taaacgaaat aagcccttcg gtttttgcga aatcattcaa taaacgcttt
     1561 ggagtaattt tatattgagt aaaaaatttg ttttaaattt tatactaaaa aggagtacta
     1621 catgagaata ttcagcttat taataacatt agttttttta ctgatgaatt gcaaagttat
     1681 cgcacaaaat caaactttag aacaaagcgt catggtggat atgataaacc ctagatcatt
     1741 ggatcatcct atctatttat cattcaaaga cggtgattat cttacatcaa ttattgaatg
     1801 tgataaatgg gattctattt atagaggcac aaattcagaa gatgctttta tggaacaaga
     1861 agcctattac aattcttttg taggaaatta ccaaaaagct cttgaaaatt tcgacaaaat
     1921 taatgccaaa tggattaatc agtcggatac tactaactta cttgagttat cttttttgtc
     1981 agcaaaaaag gtagatgctg taaatgaaat tttaagagaa gcggtaaacc accaaattac
     2041 gattgttaat gaagcgcatc atatgccgca gcatcgcgcc tttatatcgt cactacttaa
     2101 acctttatat gaatccggtt atcgttattt tgcaacagaa atgttaactg ataatgttga
     2161 cgctgtgaag aaagacggtt atgttagctt tcaacagaac gggttttatc cgaatgaacc
     2221 cgtatacgct aacttaattc gaaaggcact tgaaattgga tatactttag tcagttatga
     2281 agatactgtg aactgtgatt ttgacccgac taatccactt gcatgtttta atcagcgaga
     2341 acaaaatcaa gctaaaaaca tatccaattt aatcttaaag aactattcaa atgcaaaaat
     2401 tcttatacac tgcggttacc agcatgtgga tgaaattgaa catcaatcac ctaaggtcaa
     2461 aacaatggca gcgcatttga aagagttaag cgattccgat cctttgacaa ttgatcaaac
     2521 tgtaatgagt gaacatggct cagttaattt tgaaaacttt tattacagat acgctcaaga
     2581 tagcttaaaa ttaaaatcac caacggtatt taaatcgaac agtggatatt gggttgatga
     2641 cactaaacgc ggatttgtcg acatgcaagt ttttcaccca aggacaaatt atatcaacgg
     2701 tagaccaaac tggctcattc aagaaaacca cattctcact ttaccggatt ttgatatttc
     2761 ctcttttcct tttttggtac aagcgtttaa aaaatctgac aaaactcact caattccaat
     2821 ggatcagttt ttagttaatt cgagaaaaaa aattgcagta gccgtaccca aagggtatta
     2881 tcggatacgc attattaaca acaaaggcac cattcatgaa ctcgaagtaa aaaatcctta
     2941 gatataattc taagccttca caattttaag acggagacaa acaaaaaaag atttgattaa
     3001 aatggcgttg atttgttaca gcgatagtaa ttttactggt gccgcaaact tttttatcaa
     3061 agccacgaaa atagacacaa cagatggtgg gttgtactac aacgtagctt gttgtttttc
     3121 gagaattgga aaaattgata aatcttttga atggcttaat gtcgctatcc aaaaggggta
     3181 caacgattac cagtggataa ataatgaccc cgatttattt aatgtcagac aagatcgacg
     3241 ctgggaagaa tttataaaaa taaaaaaatg aaaagggacc aaatgaatat taacatcgca
     3301 cttatcggaa tgattgtatt gattttgcta gagaccggtt gttctcaaac tcattcattt
     3361 atgagcgaaa aaattgaagc ttgcgggcta agtgattcat tgatgattga cgaaaagcaa
     3421 ctctttaaaa aaattgatag tctgattgaa ttctctcgct gtgatacttc tgtaatattt
     3481 ggtatgaatt tattcaaaaa gaacgaaaaa aaatctgact ggattgctat taagctatat
     3541 gcgcgaagac ccaaacagcg agtcttagat cgcttagaga tgttaaaaaa atccgaccaa
     3601 ctttacagga aaaaaattat agacagtatc ggtccaagca aaggatgggc ttctgaagaa
     3661 gtgcggcaat tgtggataaa acaagctgaa attgattctc taaattggtt aacctttaat
     3721 tccaaaatta tagatcatta tggatggccg aaaatcagtg agtatggata tgaagcttca
     3781 gcaacagcat ttcttattgt tcaacataca aacgccaaaa accaagagca gaagttgcct
     3841 ttattgatga agctagccga cgagggtgag ttttctatgt cagagatagc actcttgatt
     3901 gacaggataa aagtatctaa aggtgaattt caagtatacg gtagtcaagt cgaatttgat
     3961 gcaatatcaa aaaaatttag atttttacca atcgaagacg agaaaaatgt cgatgtaaga
     4021 cggacaaaag tagggcttcc ccccatacag gaatacgcga aaagatggaa catagattat
     4081 tcttacatta aataatatta gcgtttaaat cagacttact attttaagca aactatcaac
     4141 tcgcgttagc tattcctgtt tgccaataga ttcagaacaa tgctgtggaa attcactttt
     4201 tatatcaaat atattagttt gattccagaa tcaagctatg tcatgattac cgagtttata
     4261 taactaacca atcaaacaaa ctgtcaaaac cggcaaaatg taaatacgat ccttacgagg
     4321 caaagtggca aggttgattt atatttcaat atttcttttt tttgggttta caggatgcaa
     4381 aactgtaaac atgagtttgt ataatgacat cacatgcgat tattattgtt tctattacaa
     4441 tgattctttg aaaattagta ttagattctt tggagattat acttttaata ccccatctga
     4501 ctgggattca gttaaaacaa caaaactcgc gttacaaagg ataaataaaa tcaatgattt
     4561 ctctttatct gaaagcgaca tcctcgtttc gacgcgtaca gtgattgaac cttatttttc
     4621 ttcaataata tctttaatac catcttcaaa gattagattt gatcacttaa aatccttcga
     4681 ttctttgggt acagataaac aagggcacgc gttatatcaa aacatatcgc cacaagatag
     4741 tttctgttta tttcaaaaat tgataaagtt aaataattat tggctgcacg taacagggca
     4801 aaccatcaat tcaaccgacg gctcgtgtta ctcttcgtct ttagttgatg aatataataa
     4861 aattagcaat agcattgaat atataaaacg aagtgagaga gctgctctta aaaacccata
     4921 tcttattgcg gacagcttat ttaattctga ttctaatgga aactatatca aaccaattga
     4981 ttacctcaaa tctgaaaaaa acgtatatga gaattcttca ctacggaatt catatgtcca
     5041 atgtttggaa acatacaagt cgtttgtagg gatgacagaa ccttctcttg taccaataaa
     5101 tgtctatttt ccaaacccga ccaattcatt tgagccgttc tcttcagagt ttggaggtat
     5161 tgttgatact attatttctc taacgtctaa caataaaata ataatattta atgaaagtca
     5221 tatttatcca tcacaaaggt tattgttatc tggattgttg aataagcttt atgaagcggg
     5281 attcagaaaa atagcgctcg aggctgtatg gaatgataaa gaaatcaatg caattggttt
     5341 tccaacgctt aaatcagggt tttacactcg tgaaccacaa atggcaaacc tgattcggaa
     5401 agcaaaagaa attggatacc aagtcatttc gtatgaagac ccaacattgg gaaatagaga
     5461 gtacaatcag gccgttactt tgagtcgtga attccggaaa gacagtacag cgaagatgat
     5521 tgtgttatgc gggtcatacc atatactaga aaagagatct gattcatcta gaacatggat
     5581 ggctgagcat ttaaaagaaa tatcaaaaat tgatccgctt actttcaata ctgttttttt
     5641 tgaaaatgtt tatttcgata acccgcgttt actgagtctt atttcgggat ccaaactcaa
     5701 tgaaatagct ccagttggtt ttaattttgt taacgattat tacctattta atgttacgag
     5761 tttaaaaccc tttacctgtg ataatgagaa aggtaacttc gccccttata aacccattca
     5821 aatcgttgtt cctcaattga ttaaaaaagt actattagtg caaatatttt caagcaagga
     5881 atacggcata cgcggcagag tacctgttgc ttcacattat gttgatagcg agaactgtca
     5941 aatgacatat tggcttcctc cggatgagta tgtcgttgtg tattacgggg catctggtga
     6001 aatcatatta gttaataaaa tcatcttaag ttaggagtta agtttgtaca gaattatgat
     6061 gtataaagac agtaataatt acatagtttc ttattttttt aaatttgaaa actattaggc
     6121 tttcaattgg aaagcgctat aaaaaaacgt gcgaggttac ttgacataat ctactaatct
     6181 aaaaaacaat cacccttctt ttttgttatc ccttacccgt ttttccccgg ttcataacga
     6241 cataacccca acgatttcat tgttcgacaa ccgacaaaag aggtttcaga agttgtggca
     6301 tataaccaaa gacgatattc acgtttgcaa agattgtgag tttagatata tgtgccacga
     6361 ttgtcgagca tatactattg gagggcttca ggaatcaaag ccacagaaat gccgatataa
     6421 tccttatgat gcgcaatgga acgatataaa atcttcaaaa gtattacatg gccataagta
     6481 atgcagggga agtgatggaa aaccctaaaa tagagtacat cagaaatgta acgaggtatt
     6541 acacatgaaa accaaagaaa aaatggaagt gatcgattcg ccgctttttc aaactattga
     6601 aagagaacag atgaaaagcg ttcaaggagg caagccacct ggttggacaa tggcagaaca
     6661 gactgtgtac tctaacggga catcatctga agatagcgcg gcgggaaatg attaatgaat
     6721 catcaagtaa agatggggta tcatgcccca tctttacact tttctattaa actcataatg
     6781 ttcaaattaa attgcaagaa tggtattaat tcttacatac gacggagata caacttcaga
     6841 ttatataatc gaagtattaa aaaacaaaaa acatccatat ttaaggttaa actcatctct
     6901 tttttttaaa gaaaaaatca ctatcgcccc tttacataaa tttattgaaa ttaatggatt
     6961 caaatataca tccgatttgt tgagagtgat ttggtttagg cgattttatt cttatagaaa
     7021 tttaaaattc atttatccca agagaattgt taataagtca gattttccaa acttaacatt
     7081 cgagtttaat aaaataatta ggacattata cgcgactctg aatagttctc attggctact
     7141 tgaccctaga catgcctttg taaataaaat agaggtttta caaacggcaa aggaaatagg
     7201 acttttggtt ccaaatacaa ctatcattaa taacaaggcc tttttatcca aaaaagaaaa
     7261 ctcgaactta gtttcaaaat caatagctga gggagttggt gtaaaagcta agagatataa
     7321 tttttcaatg tacacaaaaa ctatttcacc tgactttatc agcaaacttc catcattata
     7381 tgtaccttct ttggtacaag agagaattaa aaaacgttac gatataagaa ttttttattt
     7441 aaatggtagg atatttgctt ctgctatttt ctctcagaac aacgagcgta caattgatga
     7501 ctttcgcata tatgacgata atcgacccaa tagaattgtt ccttataaat tacctaatgc
     7561 aattaaaaga ggaatccgtc gcttaatgaa aaaactatcg ttaaacattg gagcgattga
     7621 catggtacga tcccaaacag gggaatatta cttcttagaa gttaacccta atgggcaatt
     7681 tggaatgatt tcgcatccat gtaactacaa tctattcgag gtcgttgctg attatttgat
     7741 aagaaatgac aagtaaaaca aaaatattcc tatcaataca cttgttcatg ttattagtcg
     7801 ggtgttctat cccaactaat caatttattt cacacaatcg atttcaaaaa acgatgggaa
     7861 gagttgttta tcacaatgac agcttgagat tatcggcgat gttttatgga gattatgttt
     7921 ttcattcagg caacaagcaa cgtgagataa gtagaaaatc tatagaaatc ataaagagcg
     7981 gatataaaga gggattatac ctaacagaga aagatttaat ttttacagct gaaacgatcg
     8041 tggaacccaa attttattca gcgttgttta gtttacataa cctcagcaaa agacgtctaa
     8101 atgaaataag taaaattgct cccgactttt tctggagtga ttccgttcgt aatgtcgcag
     8161 gtattgggca aatttttact gatgctacat ctgcagtgtt tttgattggt tttacttatg
     8221 atcatttgac tgttagaaat aaaaattctt tacaacagca atttttcttc aaccaaactt
     8281 taaatgcatt atcacgcgag tttagagacg ctgacgagtc catttttaaa tctgtactca
     8341 gcggaagcaa ttataaattg tttgaggctg aagacccatt tattttagct aataaatatt
     8401 atcaaactga atattcggga aattatcttc tacctattaa tgaactactg gaatcagaac
     8461 cgaactatgt cagtacgcaa gatcgatata aatatttaca agcgctattg acctatatgt
     8521 cttttattga tcacccccaa tatgattatt acaataaaaa aagaaagcaa atacagtcaa
     8581 gatcaatcat caattattct aaacgtttta acttaaatat tgatgaaata caaaaaacca
     8641 ttttagactc ttcctcgcat cagcgcgttg tcatgtttaa tgagtctcac tatgtaccgt
     8701 caattcgatc ttttattatg actttgctag acggtctata tcaacaaggg ttccgttatt
     8761 tggcgttgga agcattggaa gaagatcgcc ttgttaatga aaaaaagttt cctgtcattt
     8821 cttcaggttt ttattttcga gatttccgca tggcaaatat cgcgcgtatg gctaagaaat
     8881 tggggtacca tttgataaaa tatgattcgc gagaaaccaa cagagaggaa aagcaggcgc
     8941 agcgtttagc acagctaata gctaacagcg ataccaataa aattgtagta ttatccggtt
     9001 tttcacatat agacgaaaat cctgataaaa agagaatggc ttcgatctta aaaaaaattt
     9061 ccggcataaa cccctttacc atcaatatga ctgatatttc tgaaaccaga attgaattga
     9121 attctaatat ttgttttaca aataactcta cagataggat acagttacca tcatatttta
     9181 tgaacaacga cttatacgta tctatttgta atgacgactt cataaacgac gcttttactt
     9241 ttcataaaca tgaaacgtta aatatccatc tggatttttc cggtatcgcc aagcgcggcg
     9301 aatatgtttt gcttgtgttt gatatatcgg agtctccaaa cgaagggcaa ttacccgttt
     9361 ttgccaagct cgttctccca aaaatgcatg agcaaattga tataaaacta ttgcctggac
     9421 gctataatgt tttactaaaa tcagaagatg gattaatttg tcacgatact actttatacg
     9481 ttgaagtaaa taaataacga tttaggattt cgatacgccc atattcaaca tgtgaacttt
     9541 cacaaactat ttcttacaaa tttgcagaag gaagcgtttt tcacagaatc acctattgaa
     9601 gaaaagaaac ctgtaatcag tttacagatt tagctggagt ggggtatcaa attttacgtc
     9661 taatcaacca tgaactgttc taaacatctt aattataaac gctcctcgcg caaaacaata
     9721 gtgcttcatt ggttattaag tggccacttt tttttatatc acattaacct ttggtcagaa
     9781 agtagttaat caacaagacc catcgagtat ttgtattcgc tttaaagtta aggtagatac
     9841 aaatgaacct gatttaaaaa agaaatccat actatccaaa attatttaaa cgtatatcct
     9901 gaaaatggtt tcgttttttt tggagttata aagagcgata tcctcatgaa attgcactgc
     9961 aatctgtggt attatgccca gtaagctgtt gatcgagggg ctatccacat gacttggcgg
    10021 aggtcacaac aaaaacttta ctttaaatat ttagtagaaa aaattcgacc taaacttcaa
    10081 tctcaggaca gtttgtttgt tgcgaaaact atctccggtc cttatggaaa tgtggaagaa
    10141 tacaatacgg taggaggtat tattagccat cttctctttg gaaaaaaaga cgatgcaaat
    10201 ttcatttctt ttttgaaata aataaaagaa ctgtttactt tgtttgaaaa ggaatttggc
    10261 attaaaaagt attattggtg aaattttatc tgatgaaacg gataaaatat cttcgaacta
    10321 accgttttag ttttgatctt attttaaaga aaaagaagga gctacactac actgtcagat
    10381 caagttgaat caaatgatga tagtataaga agcttaatgt tacccaatgg tacataggaa
    10441 ttaagagtta tagatttcaa aggtaaaatt atagaaaaaa cgattgtaga ggtaaaatga
    10501 gtaagaagtt ctcattagct ttggttttct atgtttgttt tgcaactgta aaatcccaag
    10561 atattaaaaa cattgatgaa caagctacag cattgtattt aaaaaaagaa tatttgaaat
    10621 gtgctgaaac atttcaaaaa gcaatcgccg cagattcaaa ttactttagc gcttattata
    10681 atacggcatg ctgctacact ttggctggca aatttgaatt gtcaattaaa tttttaaaac
    10741 aggctattga tcgcggattt tctaacgagc gcatgctaaa aacagatcca gacttaaatc
    10801 aactgagaag ccgcgacgaa tggaaagaaa ttttagagta ttttcataaa gtgcgatact
    10861 gaaatatatt tcgagtaaga tttccgagtt tttttcaata caataaaatt ataactctgt
    10921 gtaaatccgg atgaaaacga tggcattata ccctctaaac aagttctaat acacctaaca
    10981 tgtatagagg gttgtatttc tttctgaccg aagctataaa ctacaaaata tactcatgac
    11041 ccgtttgcgg gcgagttgcc aaatcaaaca aacactatga agaacatgaa gtcgttcgca
    11101 atacttatcc tgctttcatt aaaccaaata tatgcgcagt attctctccc accggcaatc
    11161 atggattcga ttcgggatat tgataggtca ctatcttctg atcgtaacta tatagccgct
    11221 ttgactcggt taaaccgaat tcatgcaatg ccacaatatg ccgggcagcc tatattgttt
    11281 aatcatttgg gcttgtataa ttctttagtg tccgattatt cgacggcggt tgcttttttt
    11341 gactccattg agcttcttcg tcagagtcaa cgccatacca catcattgcc gccaaccggt
    11401 ttaacctttg atacaagcca aatacgcccc tttcccgcga tggaaaccat acttcgcctt
    11461 gcaaagaata attccgtcgt aatgatcaat gagcgtcatc acatgcctat gcatcgggct
    11521 tttattttgg aacttctgat tcgcctcaaa gcgctaggat atacccacta tgctgccgaa
    11581 ggattgcaaa atcacgatac aatcacaagc cctgcaacat accctgcgat aggaaagaca
    11641 ggatattatg taaatgagtc ggtatttggt gaattgatac gaactgccat tctgttgggt
    11701 tataagatag tcgcctatga aacaacaagc tcggcatctc ccgaatccga tcctatcaaa
    11761 aaccaaaata aaagggaaaa agaacaggct gaaaatttgt acaataaaac tttgcgccaa
    11821 gaccctcatt gtaaagtttt agtccatgtc ggttatggtc acaacgataa aatgggagtt
    11881 ggcggctgga tacctatggc atcctacttc aaaactataa gcggcgtcga gcctttgtcc
    11941 attgatcaaa ttgaaatgtt tgaacaccgt gttccgtatt ttgaaaatcc ctattaccgg
    12001 tttatatccg gtatgatttc gcagccatcg gtttttattg aaaactcttc caatacattt
    12061 tggtcgtacc tacaacgagg tagttacgat ctggaaatat gccaccccaa agaaacttat
    12121 gttcatggac gcccgacatg gagaaacata gctaaccgct atcggatgta tgacctaccc
    12181 attagtaaaa aaacaattgc aaaaggattg ttacaggcgt tttatcatga tgaaccttcg
    12241 gatgctgttc cagcagatca gatcgttttt aataccggcg ttcaacagat gattccttgt
    12301 ttattcttac agccaggaaa ataccggttc aaattagtga gtgaggatag caccataagc
    12361 acggaatggg ttgatataat caaataaaca tgaacagctt tcccatatat cttcagcatg
    12421 acaaaatgga ctgcggccct gcttgtatcc gtatgataac aaaatttttt ggacgtacag
    12481 tcagcccgga aaaattacgc cgcatcacct atgcctccaa gggaggcacc tctcttttga
    12541 atttgtcgga agccgcggaa tctttgggtt ttagaacact agcggtcaag gtatctctcg
    12601 aaaagcttca gaaagcgcct ctgccttgta ttgtgttttg gcagcagcgc cactttgtcg
    12661 tagtatataa gatcggtcga aggtatatcc atgttgccga tccggcgcac gggattgtaa
    12721 aataccgtca cgcggatttt gtatccggat ggtatagtga ccgatccaat gaaggtgagg
    12781 gtcagggtat cgccctgctc ttcgaaccta cgccggcatt gtacgaatcg gaggatgatt
    12841 ttgaaaaatc gtcgaaaccc aataaaaagg gctttggcct tttatggccg tatttgcgat
    12901 cgcatcgcaa gctgatattt cagctcggtt tagggttttt attgggaagc atattacagc
    12961 tcatatttcc gttcctcgcc caatctatgg tagatatggg tattcaccat cagaatatcg
    13021 gttttgtata taccatactt ttagcgcaac tgatgctttt tctcagtcgg atcactgtcg
    13081 aaagtatccg gcgatggata ctcctgcata tcagcacgcg gctacaaata tctcttatat
    13141 ccgactttct gatgaagctg atgcgtttgc ctgtggcttt ttttgacgcc aaacaaaccg
    13201 gcgatctgat gcaacgcatc catgacagcc atcggataga aaactttctg acagtgactt
    13261 cgctcagttt tgttttttct attttcaatc tttttatctt cggcgccatc atacttttat
    13321 acagcccttt catatttctg atttttacaa ctttcagcgc cgtttcgata acatggatca
    13381 cgttttttat gaaaaaaaga cgtgaactgg attacaaacg atttgatcgt atgtccgaaa
    13441 accagcaagg catactccag atcatcatgg gtatgcagga aatcaaactc agcggctcgg
    13501 aaaagcagaa acgatggaaa tgggaatccc tacaagccaa actttttacc attagccgta
    13561 aagcgctggg tttggggcaa gtacaggaga ccggtacgct ttttatcaat gaacttaaaa
    13621 atattatgat cacattcatc gccgccaaag aaacaataga cggacatatc acgctcggtg
    13681 cgatgatggc gatacagtat atgatcggtc aactgaatgc acctattgat cagatgatcc
    13741 tttttttgca acatgcccaa gatgccaaac tgagtttaga acgattatcg gaaatacata
    13801 cccacgacga tgaagaccat gagagcgata acagcgctca actgcctcag gaacgatcca
    13861 taagattgga tcgggtttca tttcagtatg aaggtcctcg ctcgccctac gcgctcaaag
    13921 atgttaacct cccgatacac gaaggaaaaa tcacggctat cgtcggtgaa agcggtagcg
    13981 gtaaaacgac attacttaaa cttttgctta agttttatac cccgacggaa ggaaaaataa
    14041 gcgtcggatc ggatgcactg tcgcagatca gcgcctcttt ttggcgccgg cagtgcggtg
    14101 tcgtcatgca agacggatat attttttcgg atacgatcgc tcacaatatc gctccggcgg
    14161 atgaaaccgt agattaccga aggctaatac aagccctcga ggccgctaat attcgaacat
    14221 tcatcgaatc tttacctcta cgatacaata ccaaaatcgg aaatgaagga ctcggactca
    14281 gtcagggcca aaagcaacgc attcttatcg ctcgcgcagt ttataaaaac ccgcaatatt
    14341 tgttttttga tgaagctacc agcgcgctgg acgctactaa cgaacggatt attatgtaca
    14401 atctccagcg cgtcttccaa aacaaaaccg tcgtcatcat cgcgcaccga ctcagtactg
    14461 ttcgcaacgc cgatcagatt atcgtactag aaaaaggaaa cgtggtagaa tccggttcgc
    14521 atatagaact gacccaacaa cgaggcgttt acttcgagct ggtacgcaac caattagaac
    14581 tcgggaatta gaagatgtcc aacgatatcg aaccccttga acgtattcac agcgacagct
    14641 atgacgacgt gatgaattac gagccgcatt ggttgatacg gtggggctcg tttatattgc
    14701 tattgatcat tatttttttg attacggtca gcggatatat acgatacccg gatatattac
    14761 gcggacaaat cacgcttatc agcgatcaac ccacattgga ggttatcgca cagtccgcag
    14821 gccgcgtgaa actgtttgtc aaagatcgcg acacggtcaa agttggccaa cctttggcta
    14881 tgatcgacaa tcccgccgac tttgcatcca ttcggaaatt gcgcttattc ttagattccg
    14941 catcatctca gagtgatagt tatgtaatga tagatacggc gttacagctc ggagaactgc
    15001 aggcttttta ttcggaagtg atacaaaatt ataaagaatt agtttcgttt aaagcgctca
    15061 attatgaagt aaaaaagatt aactccattc ggcaacagat caacgcctat tatactttgg
    15121 gcgaccgact agaagatcag atcaaacttc tgaagttgga tgtgacaaaa gcggaaaaag
    15181 attttgaagt caataattct ttacatgaac gcggacttct gtcggaggtt gaactttccg
    15241 gttatcaatt tcagttaaat caaaaaaaaa tagcgctaga ggaagcccaa aaagaggcca
    15301 ttcgcaattc gatcgtggtt tctgataatt taaaaaacat acttgatatg gaatttcaaa
    15361 atcaggataa aaccgtacga tatttgatcc gcatacaaga atctatgaga aaattgagaa
    15421 gcgaattatc agcatgggaa aaaaaatact ggatgaccag tcctgtcaac ggcgaagtca
    15481 tctataatga tgtatggaca gacgaccgat ttgtaaaact gggccaagaa atcatgacga
    15541 tcaatccgaa gacaaatcgt attcgtggac gcgtaaaata tgaaggtcct gggctcggaa
    15601 aagttgttcc cggacaggaa gttaaaatca aattagataa ttaccctttt caagaatttg
    15661 gcatcgtacc aggcatcgta gaaaatattt cagccgtatc caaagataaa tccgtcgtca
    15721 tacaaatcac gctcccacaa caattaaaaa catctttcgg cgccttgttg gagtacaaac
    15781 aagaaatgca aggaacatcc gagattatca ccgaaaaccg cacgttgctc gaaaggattt
    15841 ttcaacaatt gcgccaaatt acccaatatc agtaaattaa tttgtatttg ctcgtggttt
    15901 ccaaatacat gacttcttat ttttttaaat ttgaaaacta ttaggcttcc aattggaaag
    15961 cgctataaaa aaacgtgcga ggttacttga tataatctac taatctaaaa aacaatcacc
    16021 cttctttttt gttatccctt acccgttttt ccccggttca taacgacata accccaacga
    16081 tttcattgtt cgacaaccga caaaagtcat tttttgcgat gatacgaatc attccaaagt
    16141 aatcggattt atgatagact gctcttagtc gggtgctttg tgcacggccg gctattgtcc
    16201 aaccaacgag gctgttatgt ccaacaccaa acgcgcgatg gttacgctgg acggcaatga
    16261 agccgccgct tacgtagccc acaaactcag cgaagtcata gccatctacc ccatcacgcc
    16321 gtcttcggcg atgggcgaat tttccgatga gtggtccagc aaaggaaaag ccaatctttg
    16381 gggaaccgtg ccgctggtca tggaaatgca gagcgaaggc ggtgcggcgg gaactgtgca
    16441 cggcgcattg caaaccggtt cactcacgac gacatttact tcatcgcagg gactcctgct
    16501 gatgattccc aatatgtata aaatcgccgg cgaactcacc tcgacggtat ttcacgtcgc
    16561 ggcgcgggca ttggcttcac aagcgctttc gatattcggc gatcactccg acgtgatgtc
    16621 cgttcgcgct acaggatggg cacaactgag tgcacattcc gtacaggaag tgatggatat
    16681 ggcgctcatc gcgcatgcgg ccacgctccg cgcacgggta ccgttcattc atttttttga
    16741 cggatttcgc acgtcgcacg aagtcatgaa agtagaacaa cttaccgacg aagacatgct
    16801 cggtatgatc gatgatcacc tcatccgtgc gcatcgcgag cgcggtcttt caccggaaca
    16861 tcccgtcatg cggggcaccg cacaaaatcc ggacgtattt tttcaagggc gtgaagccgc
    16921 caatccctat tatcaaaaat gtcccgcgat cgttcaggaa actatggatc gttttgcgaa
    16981 gctcacgggt cgcgcctaca aattatttga ttatttcggc gctccggatg cggaacatat
    17041 tatcgtcgcg atgggttcgg gcgctttgac ggcacaggaa accgccgcat tcctgatcgg
    17101 gcaaggccat aaagtgggcg tagtcaaagt tcacctctat cgcccgtttt cgatcgaaca
    17161 tcttatcgcg gcttttccta aaaccgttcg caccgtcgcc gtgctggatc gcaccaaaga
    17221 accgggcagc gcaggtgaac cgctgtatca ggatgtcatc acggcgctta cggaaacttt
    17281 tatcgaagga aaattgccct tcgccatgcc ccgtgtgatc ggcggccgct acggcttatc
    17341 atccaaagag tttacgccgg ccatggtcaa agccgtttac gatgaattgc aaaaacctct
    17401 tcctaaaaat cattttaccg taggcattca tgatgacgta acgcatttga gcctggatgt
    17461 gaatcccgat ttttcgatcg aaccccaaga tgtcgtgcgg gcattgtttt acggactcgg
    17521 cgccgacggc acggtcggtg ccaataaaaa ctctatcaaa atcatcggcg aggataccga
    17581 taatttcgcc cagggttatt ttgtgtacga ttctaaaaaa tccgggtcta tcaccgtttc
    17641 ccatttacga ttcggcccga agcctctgtt ttcctcgtat ctggtgaccc gtgccaattt
    17701 tatcgcgtgc catcagttta gttttcttga aaaatacgat atgctcaaat atgccgtcga
    17761 aggcggtacg tttttactca atagcccctt tgacgaaaat gaagtgtggg atcaattgcc
    17821 gctcatcgca caaaaagaaa ttacggaaaa aaaattacgc ttttttgtga ttgacggtta
    17881 tcgtgtggcc aaagaaaccg gcatgggcgg acgatttaat actatcatgc aaacttgctt
    17941 ttttgccata tccggcattc tcccgcgcga taaagcgatc gaagcgatca aacacgccat
    18001 tcaaaaaacc tacggtaaaa aaggcgatga aatcgtacgc atcaacttta acgccgtaga
    18061 tcagactttg accaatctca aagaagtcaa ctatgccgga aaacctttga ccgatcgtgc
    18121 gatgcggcct ccggtggatc ctctggcgcc ggacttcgtc aaagacgtaa ccggccgtat
    18181 gatactaggc gacggcgaca cattgccggt aagcgccatg ccgcccgacg gaacattccc
    18241 gaccaatacc gcacgttggg aaaaaagaaa tatcgccctc gaaatcccgg tctgggatcc
    18301 cgccgtgtgt atccaatgta acaagtgcgc attggtatgc ccgcacgcaa cgatccgcgc
    18361 taaagtgttt cccgaaactg cactcgacgg taaaccggaa catttcaaag ccgtaccata
    18421 taaaggtaaa gaatttccca acagccttta taccatccaa gtggcccctg aagactgcac
    18481 cggctgcggc ttgtgtgtcg atgtatgccc ggcaaaaaac aaacaggaaa cccgcctcaa
    18541 agcgatctat atgcagccgc aaagcccgat tcgcgatcaa gaacgcatcc attgggaata
    18601 tttccttcac ctcccggaag ccgatcgtac acaggtgccg ctcaataccg tcaaaggttc
    18661 ccagttatta caaccgctat tcgaattttc cggtgcatgc agcggctgcg gcgaaacgcc
    18721 ttacctcaag ctgatcagtc agcttttcgg cgatcgcgca atggtcgcca atgcgaccgg
    18781 ctgctctagc atctacggcg gtaatctgcc tacgacacct tgggcgcaaa ataaagacgg
    18841 acgcggcccg gcgtggagca attcgctgtt cgaagataat gccgaatttg gtctcggttt
    18901 ccgtctcacg gtggacaaac aaacagcgtt tgcgcaagaa ctgctctatc gcctcggcga
    18961 ctcattggac aaagaactta tcgcatcatt gatcgcgtca ccgcaaaaaa ccgaagccga
    19021 tatccaagcg caacgcacac gcgttcaagt tctgaaaaac cgccttaccg cattatcaca
    19081 tcccgatgcc aaacgtttac tcgacgttgc cgattttctt gtcaaaaaaa gcgtctggat
    19141 tgtcggcggc gacggctggg cgtacgacat cggttacggt ggcttggatc acgtactcgc
    19201 atccggacgc aacgtcaata tcatcgtgct ggacaccgaa gtgtattcca acaccggcgg
    19261 ccaggcgtct aaatccacca atcgcggcgc ggtagctaag tttgccgcct cgggaaaacg
    19321 ttattccaaa aaagatctcg gcctcatggc gatgagcacc ggccatgttt atgtcgcaca
    19381 gatcgctatg ggcgccaacg at
//
LOCUS       DAOQNQ010000033        19310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_997_length_19310_cov_6.453441, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000033 DAOQNQ010000000
VERSION     DAOQNQ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19310
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_997_length_19310_cov_6.453441"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2203
                     /gene="gcvP"
                     /locus_tag="PK754_03840"
     CDS             <1..>2203
                     /gene="gcvP"
                     /locus_tag="PK754_03840"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNB08665.1"
                     /translation="DGAIYDYAAFTQQCHAQGVYVAVAADILALTLLTPPGEWGADVV
                     VGSTQRFGIPMGYGGPHAAFFATKDEFKRKMPGRIIGVTIDANNKRALRMSLQTREQH
                     IRREKATSNICTAQVLLANMAGMYAVYHGPKGMREIGLRVHNRAVTLAGALLSAGYRQ
                     LNAAYFDTIKIDTGDAKTYDAIRSAALAAQINLCYHGSQTITIAVDETTGAKDIEILT
                     QIFAKAKSASVTVKHSDLQTPSWGKPFVRQSAYLTHPVFNTYHSETEMLRYIKYLELK
                     DVSLANSMIPLGSCTMKLNATTEMEPVTWAEFGGLHPFAPSDQAAGYQQLFSELESYL
                     NAITGFAGTSLQPNAGAQGEYAGLLVIREYLKSIGQSHRHVALIPSSAHGTNPASAVM
                     AGMQVVVVKCDDMGNIDVKDLRTKSEQYKNELAALMVTYPSTHGVFEEAIHEICEIIH
                     ANGGQVYMDGANMNAQVGLTSPAMIGADVCHLNLHKTFCIPHGGGGPGMGPICVAKHL
                     VPFLPGHAVVKTGGNKAMTAVSAAPWGSASILLISYVYIRLMGAAGLTDATKYAILNA
                     NYIRARLKDHYDVLYVGKNGFVAHEMIMDTRGFKTTANIEVIDMAKRLMDYGFHAPTV
                     SFPVAGTLMVEPTESEPKSELDRFCEAMISIRNEIREIENGQAHKEDNVLKHAPHTAE
                     TVVSDTWTHAYTREKAAFPLPTLRTNKIWPSVGRVNDAYGDRNLICSCPPV"
     assembly_gap    2204..2303
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2421..2705
                     /gene="rpoZ"
                     /locus_tag="PK754_03845"
     CDS             2421..2705
                     /gene="rpoZ"
                     /locus_tag="PK754_03845"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNB08666.1"
                     /translation="MPVKAFPFDKIDASIENVYEAVIVAARRARQINDEQMIHVRTLT
                     EGDEAMDGEEVKINRDDILDLEKLPKPVATALDELLAGKIKHEYLNREEE"
     gene            2806..4029
                     /gene="coaBC"
                     /locus_tag="PK754_03850"
     CDS             2806..4029
                     /gene="coaBC"
                     /locus_tag="PK754_03850"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNB08667.1"
                     /translation="MANDSEKYSGKKILIGITGGIAAYKIAELIRYFVKSGAEVRVVM
                     TKNATEFITPLTIETLSNNRVTTDTFPGHDAHYPEMWGTHHVNIAQWSDIMLIAPAGG
                     NVIGKIASGIADDALTTIVMACSKPVALAVAMNDRMYTNSIVQQNIKKLRDHGYHIID
                     PGNGFLAEGYEGVGRLAEFESLIWEADKILLGTNELKNKRILITAGPTQEPLDPVRIL
                     TNHSSGKMGYAIAREAALMGAEVTLISGPTALPAPPGVSVMQVTTADAMDKAVRGQFT
                     KHDALIMTAAVADFKPARQEKQKIKKTGAKNITIELARTPDILANIGKQKGRRYVVGF
                     ALETDQEIENAQRKLHAKNLDFIVANNPKEQGAGFNTDTNIVTIIDKNSIMRWPKMSK
                     AQVARKILEKVAKEI"
     gene            4035..5504
                     /gene="gltX"
                     /locus_tag="PK754_03855"
     CDS             4035..5504
                     /gene="gltX"
                     /locus_tag="PK754_03855"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNB08668.1"
                     /translation="MSVRVRYAPSPTGFLHIGGLRTALYNYLFARHHGGKFILRVEDT
                     DRTRLVEGAVDDFMNMLAWAGIQVDEGPGIGGSFGPYYQSERLHLYKQYAEQLLAEGK
                     AYRCFCTAERLDQMRKFQEKSKLPPKYDRTCLRLSAEEINKKIAAGEPHTIRMRVPDN
                     TVIRFKDHIRGDVEFTSDNVDDQVLMKSDGYPTYHLANVVDDHLMEITHVIRGEEWVS
                     STPKHVLLYQYFGWKAPEFAHLPLLLNSDRSKMSKRQGDTEARAYPPKGYLRDALVNF
                     IALLGWNPGDDREIFSMDELIKEFSIERIHKAGAVFDIQKLNWINTMYIRKLSDDEIY
                     AILKPDLEKAGYSDYPKQYVLRAIGLMKERVNFISEVLTFSSYFFRDPDTYDPEGVRK
                     RWTPQAKTYLTELAGRLENTASFIHADIEAVFRGYSEELKIKASELIHPVRIAISGMT
                     LGPGLFELLEVIGKEAVIRRMRRAIDVYDHVVGEAQSVK"
     gene            5698..6687
                     /locus_tag="PK754_03860"
     CDS             5698..6687
                     /locus_tag="PK754_03860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNB08669.1"
                     /translation="MEKSIAPEHPIHVSSQTDIKTAALTVHDNKIAMRWSLFIGFGMF
                     AMKIYAYFITGSAAILSDAAESVVHVLAVSFAAYSLWLSTQPPDKNHPYGHDRISFFS
                     AGFEGAMIVMAALYIIYESVKKWIRGLEIHNIESGTMFVVIATAVNAALGWYLVHKGK
                     KNHSLILIANGKHVLTDSWTSFGVIFGLVLTMVTGWLPFDPIVAILVALNILWSGGKL
                     IRQAFGGLMDEADPETQTKLESILQAETQKYGIRFHDLKHRSSGNRIIIEFHLLFHDS
                     LTIVQAHAQATAIEKVIADTFPIETEITSHLEPIEGHNEAHITNTRMRNGKNE"
     gene            complement(6700..6990)
                     /locus_tag="PK754_03865"
     CDS             complement(6700..6990)
                     /locus_tag="PK754_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNB08670.1"
                     /translation="MKPITAIATQTEHEVSESTTLDHPLRVILFNDDIHTFDEVIAQV
                     IKAIGCTVQKAEAIAWEVHTRGKAVVYEGDLGECLRVSSVLEEIGLHTQIEP"
     gene            complement(7083..8939)
                     /locus_tag="PK754_03870"
     CDS             complement(7083..8939)
                     /locus_tag="PK754_03870"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNB08671.1"
                     /translation="MMTWIREKTHWLLYALIVFFIALIVIEWGANYTDIANKSRGTIG
                     KINGEDIRYADFATVYNNQINNARAQKDGEALTDAEMEQLSDQIWNQIVEEKLLREYM
                     KENHVAVGDTEVVFELRNNPPEFIKNTPAFLTDGNFDMNKYQQALNNPQYAKEWAQVE
                     QMLRMQMPYNKLQAMIATSPRISESELRQEYARRNIKLNGQIVFFSPADIPASSVQVT
                     DAEAQSYYDNHKDDYKQEEKARLAFVSFSTEATKEDSAEVIERVNEVKKQVLEGKDFA
                     ELAKLNSVDRAANQQGGSLGWFTKGAMVREFEEACFNAKKGDVIGPVITSFGYHIIKI
                     DSTRFVKDKKARAKKAGEAEDRDSVLARHILIRMEASQTTIETARENANAFYETAKDN
                     GFDKALEKYASRFNLTSDTTLDIVNNENGIVAGFAERMKNVTRFAFTEKAGAVHRPHQ
                     TGRGVTIFAIVSRTDKGIQPFEVVKERVRNTVMEEKRMTMAYQKAQELRSKINSLADL
                     KALDSTLTVRDLTNFTMNNSIIGVGRDAKINGVLFNTTVGTLSEAIKGNRGAYIAFVA
                     TRDDINESLFRDARNDLRRQLQSTKQQRAYRDWVESMKKQATIKDFRPEFNL"
     gene            9346..10974
                     /locus_tag="PK754_03875"
     CDS             9346..10974
                     /locus_tag="PK754_03875"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB08672.1"
                     /translation="MLNNDGWTQQEGIEVLKTAIQGVAYDSGKVNLMHDLAFQYLEVD
                     LDSAEYYIRKSEELSRVFKYKFGEVKAVNYLGYVFEQRGRVDDAKRLYGDALKMAMNN
                     NDTVGLSRAYNSLAGIAMIGGKYREALGYYENMLGVYQGRADLQSQSVAYNNIGNVYV
                     EMGDHEQALEYYFKSLKIAQEAGDQKSLGTSYINLGNVFLEQSKYVEALEYYQKSLEI
                     QEKIGSQSGTALCFLNIGSVYEKQERLDSALHLYRKAYGLYKQLDNKSGVAWTLVSLG
                     DVYNRKGNYAEAATYLRQSLEMAKFVKDLTAEGNAYVSLAQNYKKLRMYYMALQYAGM
                     GHQIYLASGNRNRIRDVRLVFSEVYEAMGYLPQALTWYKGYIAIKDSLQSVGANRKTA
                     ELQLLYDSKRKDQQIALLQRDSTIKSLEISQSKIVRHSLIGGFLALIVVLVLLWQRYR
                     VKRQSEELLNNKNAELTKINSDLEEALHNVKELRGMFPICANCKKVRDDHGFWSQIET
                     YLQKHTDAEFTHSVCPECLNTLYPEFVKRAKEAE"
     gene            11544..12335
                     /locus_tag="PK754_03880"
     CDS             11544..12335
                     /locus_tag="PK754_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08673.1"
                     /translation="MKTFFLTLFLTWCGFNTLTAQDRIFNYTYQSNVLNAGQREVEPW
                     VTFKWGREKFYRAFENRIEYELGFAKNLQMAFYINAKTSAAQTNDSTIENATELSFSN
                     EWKYKVFDAVADPVGFALYGEYGIGRNEIEIEGKLIFDKRIGNSILALNLVGELEFEK
                     EAEHGEIENEKETVYELDLGYSYRVRNGLYLGIEARNHNEVVEGKWEHSALFMGPTLS
                     YTMENFWVNLTILPQVKALKGATKNGLVLDEHERLETRLIFSYIF"
     gene            12344..12643
                     /locus_tag="PK754_03885"
     CDS             12344..12643
                     /locus_tag="PK754_03885"
                     /inference="COORDINATES: protein motif:HMM:NF021165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNB08674.1"
                     /translation="MKKYFFCLSLLLMSSCAPALYVPSAATTSDPAELTVLNEGRAMY
                     VQHCGSCHSLFVPSDFSDEAWEAHLDQMQTRAKISDHQKERILKYLTSYKKPEKK"
     gene            12643..12975
                     /locus_tag="PK754_03890"
     CDS             12643..12975
                     /locus_tag="PK754_03890"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNB08675.1"
                     /translation="MSTHRFVAINYITCNDNYRERFEELFSSRAHAIDRMPGFVDMQV
                     LKPDQPDEPYLIVSHWDSKEAFDTWTQSPEFIEGHKRGFADVRAAKARGEVAPMESKF
                     KTYAVIAR"
     gene            12972..13094
                     /locus_tag="PK754_03895"
     CDS             12972..13094
                     /locus_tag="PK754_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08676.1"
                     /translation="MNFKNRWPWAKKLGIIGFLFFLIKGVLWLIVPMATVWVCN"
     gene            13121..13453
                     /locus_tag="PK754_03900"
     CDS             13121..13453
                     /locus_tag="PK754_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08677.1"
                     /translation="MADCFHRIVQQNEMGYVSVCEMCERIQVGFGTALANFTRREFSD
                     FRERIVREWMALKSEPNPREKNIFIETSSRAWHLALTGSELSKLTDLLEKADTVLSLT
                     SQRATYYN"
     gene            13464..13637
                     /locus_tag="PK754_03905"
     CDS             13464..13637
                     /locus_tag="PK754_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08678.1"
                     /translation="MKSDCCKNYKKKGKACRECPVLAKLRERHADLRRKEVFEYLQNL
                     KRNRFVCEVSYEP"
     gene            13899..14483
                     /locus_tag="PK754_03910"
     CDS             13899..14483
                     /locus_tag="PK754_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNB08679.1"
                     /translation="MTTDLKKFLSRYAGFSEAELENIAGKFKSQKLRKNSYMLRQGEV
                     CRNIVFVQKGCLRLFYRKDDVDVSVWFSFAQSSAIEIYSFISENPSDYYLQAIEESEV
                     LYLSKTELKKLYARHPNMQQMMRNFWEDVILNLIKRFTALQTDSAEKRYLDLLGKPDF
                     LGTIPQKYLASFIGVTPTSLSRIRRQITRKHKLS"
     gene            14653..15321
                     /locus_tag="PK754_03915"
     CDS             14653..15321
                     /locus_tag="PK754_03915"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HNB08680.1"
                     /translation="MYNFSYFKEKERQVIREFMEGNPFAFLTGSFLSGVQVGTQIPIL
                     FEERDGELYLQGHIMRHTDHHKALVENPNALVVFTGPSTYVSASWYSNPHIGSTWNYM
                     SVYISGKMKFMSSDELIRFMRKLTLKFEKGNTQSQTIYDNLPESFLNKMMPAIAGFEI
                     KADTLDNVFKLSQNRDEKSYMNIISKLEAQGGNSALIAQEMIKRKSELFPPGVAWDAS
                     KFDS"
     gene            15355..15795
                     /locus_tag="PK754_03920"
     CDS             15355..15795
                     /locus_tag="PK754_03920"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB08681.1"
                     /translation="MEIKKLTADEVSDFKRLIEIFKDVFENDVDIPPVDHLRRLLSNP
                     DFMVFVALSGGNVAGGLTIYVLHRYYSVKPEAYIYDVGISPEFQRKGLGKALMAEVCR
                     YCQANGFENAYVEAEHDDVEAVRFYRATAYNHEVHAVHFTYVWR"
     gene            16208..16597
                     /gene="rpsL"
                     /locus_tag="PK754_03925"
     CDS             16208..16597
                     /gene="rpsL"
                     /locus_tag="PK754_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNB08682.1"
                     /translation="MPTIQQLIRKGRQSKTWKTKSPALQECPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLSNGIEVIAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIVRGVL
                     DTQGVQDRKQGRSKYGTKKGKEGAAKK"
     gene            16630..17100
                     /gene="rpsG"
                     /locus_tag="PK754_03930"
     CDS             16630..17100
                     /gene="rpsG"
                     /locus_tag="PK754_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNB08683.1"
                     /translation="MSRRKRSPKRRIPADPKFNSVLVTKMINVIMWQGKRGVAESILY
                     DAMETVEKKTGKNGLEILEKAIKNVAPLLEVRSRRVGGANYQVPVEVRAERRQTLALR
                     WLIGYSRERAGKSMASKLADEILAASNNEGAAVKKREDVHKMAEANKAFAHFRW"
     gene            17145..19226
                     /gene="fusA"
                     /locus_tag="PK754_03935"
     CDS             17145..19226
                     /gene="fusA"
                     /locus_tag="PK754_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNB08684.1"
                     /translation="MPRSISLEKTRNIGIMAHIDAGKTTTTERILYYTGVLHRIGEVH
                     DGAATMDWMEQEKERGITITSAATTCFWKNHRINIIDTPGHVDFTVEVERSLRVLDGA
                     IALFCAVGGVEPQSETVWRQADKYGVPRIAFVNKMDRIGADFFNVVKMMKERLGAHPV
                     PITFPMGEGDMFTGVIDLIDMKAIVFDESSQGMRWDVIEIPADLKEKADNARQFMLEQ
                     IADYDEVLMEKYLNGQDITKQEIQKAIHAGTLQCKITPVLCGSSFKNKGVQRLLDAVL
                     EYLPNPLEGGNLKGHNPDDHTQVIERRIDDSEKFCALAFKIMTDPYVGKLTFIRIYSG
                     MLKAGSYALNVTSGKKERIGRVLQMHANHREDIDACYAGDICAAVGLKFTKTGDTLSA
                     EDSPILLEKMEFPEPVIEVAIEPKTKADQEKLSEALSKLAEEDPTFRVSTNEETGQTI
                     IKGMGELHLEIIVDRMFREFKVEANVGKPQVAYKEGITKKVEAEGKFVRQSGGRGQYG
                     HVWIEVEPNEAGKGFEFTNGIVGGVVPKEYIKPTEEGIKEAMKNGVIAGFPLEDVKVK
                     LFDGSYHDVDSSEMAFKIAGSMAIQAAAKKAGPIILEPIMFVEVVVPEDYLGAVMGDL
                     NSRRGQIMGMNQRKDAQVVHAHVPLAEMFGYATALRSATQGRAIYSMHFHHYDPVPRA
                     VEAQLLEKVKA"
BASE COUNT         5411 a         4060 c         4550 g         5189 t
ORIGIN      
        1 cggatggggc gatatatgat tatgccgcct ttacgcaaca gtgccacgca caaggtgtct
       61 atgtcgctgt agcagcagac atattagcgc tcacgttatt gacccctccg ggtgaatggg
      121 gtgcagatgt agtggtcggc tccacacaac ggtttggcat tccgatggga tacggcggtc
      181 cgcatgcagc tttttttgca acgaaagatg aatttaaacg caagatgcct ggtcgtatta
      241 tcggtgtgac cattgatgca aataacaaac gtgcattacg tatgtcgttg cagacacgag
      301 aacagcatat tcgccgtgaa aaggcgacga gtaatatttg tacagcgcaa gtattgttgg
      361 ccaatatggc cggtatgtac gcggtttacc acggtcccaa agggatgagg gaaatcggct
      421 tgcgcgttca caatcgtgcc gtaacgcttg ccggtgcact cttgtccgcc ggttaccgtc
      481 agttgaacgc ggcatatttt gatacgatca aaattgatac cggcgatgcc aaaacgtatg
      541 atgcgattcg aagcgcggca ctggcagcac aaatcaatct gtgttaccac ggatcgcaaa
      601 caatcacgat cgccgtggat gagacgaccg gtgcaaaaga tatagaaatt ctgacacaaa
      661 tttttgctaa agcgaaatcc gcttcggtca ctgtaaaaca ttcggattta caaacgccgt
      721 cgtggggtaa accatttgtt cgtcaaagcg cttaccttac gcatccggta ttcaatacgt
      781 atcacagtga aaccgaaatg ctgcgctaca tcaaatattt ggaattgaaa gatgtgtcgt
      841 tggctaattc gatgatccct ctcggttcgt gtactatgaa actgaatgcg accaccgaga
      901 tggaaccggt aacctgggcc gagtttggcg gcctgcatcc gtttgctccg tcagatcaag
      961 cggccggcta tcaacagctt tttagcgaac tggagtcgta tcttaatgcg atcaccggat
     1021 ttgccggtac ttcgctgcaa cccaacgcgg gtgcgcaggg cgagtatgcc ggtttacttg
     1081 tgatccgtga atatctgaaa agcatcggcc agtcgcatcg tcatgtcgca ttgatcccgt
     1141 cctcggcgca cggcacgaac cctgcgagcg ccgtgatggc cggaatgcaa gtcgtcgtcg
     1201 taaaatgcga tgatatggga aatatagatg taaaagattt gcgcactaaa tccgagcaat
     1261 acaaaaatga acttgcggct ctgatggtga cttatccttc aacgcacggt gtatttgaag
     1321 aagcgattca tgaaatatgc gagatcattc atgccaatgg aggccaggtt tatatggatg
     1381 gtgcaaacat gaatgcgcaa gtcggcctta ccagtccggc gatgatcggc gcggatgtgt
     1441 gccacctcaa tttacataaa acattttgta ttccgcacgg cggcggtggc cccggcatgg
     1501 gtccgatctg tgttgccaaa catcttgttc catttttacc gggtcatgcg gtagtcaaaa
     1561 caggtggtaa taaagcgatg acggcggttt cggcagcgcc atggggcagt gccagcattc
     1621 ttctcatatc ctacgtgtac atccgtctga tgggcgcggc gggattgacc gatgcgacca
     1681 aatatgccat tctcaacgcc aactatattc gtgcacgtct caaagatcac tacgatgtgc
     1741 tctacgtcgg gaaaaatggt tttgtagcgc acgagatgat catggataca cgcgggttta
     1801 aaacaaccgc caatatcgaa gtaattgata tggccaaacg tctgatggat tacggatttc
     1861 atgcgccgac cgtttcgttt ccggtggccg gtacgctgat ggtggaaccg acagagtccg
     1921 aacccaaatc cgagctggat cgtttttgtg aagcgatgat atccatacgc aatgaaattc
     1981 gagaaatcga aaatggtcaa gcacacaaag aagataatgt gctcaaacat gcaccgcata
     2041 cggctgaaac ggtggtttcc gatacatgga cgcacgctta tacaagagaa aaagcagcgt
     2101 tcccattgcc gacgttgcgt accaataaaa tatggccttc cgtcggccgc gtcaatgatg
     2161 cgtacggcga ccgcaatctg atttgcagtt gcccgccggt agannnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnngttatta ttagaaataa tgatgaatat atatttttct
     2341 attgcgcgaa taggacaaaa cgtctatatt ggcgtcccat ttggcaatat aagtgtaata
     2401 atttcaataa ggagttcacg atgccggtca aagcgtttcc ctttgataaa attgatgcat
     2461 cgatcgaaaa tgtctacgaa gccgtgatcg ttgcggcgcg tcgtgcacgt cagatcaatg
     2521 acgaacaaat gatacatgtg cgcacgttga cagaaggcga tgaagcaatg gatggtgaag
     2581 aagtaaaaat caaccgcgat gatattttag atttggaaaa acttcccaaa cctgtcgcaa
     2641 cggcgttgga tgaattgctc gctggtaaaa tcaagcacga atatctcaat cgcgaagaag
     2701 aataaccgcg ttttacaaaa gtttcgatgc aaattccaaa tgccggtata acgccggtta
     2761 tttggaattt gccgtttatg gatgatacag caaacctctg gcatgatggc aaacgattct
     2821 gaaaaatatt ccggaaagaa aatactgatc ggtattaccg gcggtatcgc ggcatataaa
     2881 atcgccgagc tgatccgtta tttcgtcaaa tcgggtgcgg aagtgcgcgt ggtcatgacc
     2941 aaaaatgcca cggaatttat tacgccgctg acgatcgaaa cgctttccaa taaccgcgtt
     3001 acaaccgata cgtttccggg acacgatgcg cattatcctg aaatgtgggg tacgcatcat
     3061 gtcaatatcg cccagtggtc tgatatcatg ctgatcgcac cggccggcgg taatgtgatc
     3121 ggtaaaatcg cttcaggtat tgccgatgat gcactgacga ctatcgtcat ggcttgttcc
     3181 aaacccgtag cattggctgt tgcgatgaat gatcgtatgt acaccaattc cattgttcaa
     3241 caaaacatta aaaaattgcg tgaccacgga tatcatatta ttgatccggg taacggcttt
     3301 ttagctgaag gttatgaagg tgtggggcgt cttgccgagt ttgaatcgtt gatctgggaa
     3361 gcggataaga ttttgctggg cacaaatgaa ctgaagaaca aacgcatttt gatcaccgcc
     3421 ggaccgaccc aagagccgct tgatcccgtg cgtattttga ccaatcattc ctcaggtaaa
     3481 atgggttacg ccatcgctcg tgaagcggcg ttgatgggcg ctgaagtgac gttgatttca
     3541 ggtccaaccg cacttccggc gccgccgggt gtgtcggtga tgcaagttac gacagccgat
     3601 gctatggata aagccgtgcg tggtcagttt accaaacatg atgcgttgat tatgaccgct
     3661 gcggtcgccg attttaaacc ggcacgacag gaaaaacaaa aaatcaaaaa aaccggcgcc
     3721 aaaaatatta cgatagaatt ggcacgcacg ccggatattt tggcgaatat cggcaaacaa
     3781 aaaggtcgcc gatatgtcgt gggatttgct ctcgaaaccg atcaggaaat agaaaatgcg
     3841 cagcgtaaat tgcacgcaaa aaacctggat tttattgtag ccaataaccc caaagaacaa
     3901 ggcgcaggtt ttaataccga caccaatatc gtaacaatca tagataaaaa tagtattatg
     3961 cgctggccca aaatgagcaa agcgcaagtt gctcgtaaga ttctcgaaaa agttgcaaag
     4021 gagatataaa aagtatgtca gttcgtgttc gttatgcccc aagccctacg ggttttcttc
     4081 acatcggcgg tcttcgcaca gccttatata attatttatt tgcacgtcat catggcggaa
     4141 aatttatctt acgtgtcgaa gatacggatc gtacgcgcct ggtcgaaggc gcagtggatg
     4201 attttatgaa tatgctggcg tgggccggca tacaagttga cgaaggaccg ggcatcggcg
     4261 gttcatttgg tccttattat caatccgaac gccttcattt atataaacag tatgcggagc
     4321 aattgctcgc tgaaggcaaa gcctatcgct gtttttgcac ggcggaacgc ctggatcaaa
     4381 tgcgtaaatt tcaagaaaaa tccaaattgc ctcctaaata tgaccgcaca tgtttgcgtc
     4441 tatccgctga ggaaattaat aaaaaaatcg ccgccggtga accacacact attcgtatgc
     4501 gtgtgccgga taatacggta attcgtttta aagatcatat tcgcggtgat gtggagttta
     4561 cttccgacaa cgtggacgat caggtattga tgaaatccga cgggtatccg acgtaccatc
     4621 ttgccaatgt cgtggacgat catcttatgg aaatcaccca tgtgattcgc ggagaagaat
     4681 gggtttccag cacgccgaag cacgttctgc tatatcagta ttttggctgg aaagcgccgg
     4741 agtttgccca tcttccgctc cttctcaact ccgatcgcag caaaatgagc aaacgccaag
     4801 gtgataccga ggcgcgcgcg tatccgccca agggttatct gcgcgatgcg ttggtcaatt
     4861 ttattgcttt gttgggttgg aatcccggtg atgatcgcga aattttttcg atggacgaac
     4921 tgatcaaaga attttcgatc gaacgtattc acaaagccgg tgccgtgttt gatattcaaa
     4981 agttgaattg gattaacacg atgtatattc gaaaactttc ggatgatgaa atatatgcga
     5041 tccttaaacc ggatttggaa aaagcaggtt attccgatta ccccaaacaa tatgttttgc
     5101 gtgcgatagg cttgatgaaa gaacgcgtca attttatatc cgaagtgctg acgttttcga
     5161 gctatttttt ccgcgatccc gatacctatg atccggaagg cgtgcgcaaa cgctggacac
     5221 cacaggctaa aacctatttg acggaattgg ccggccgttt agaaaatacg gcgtcattca
     5281 ttcacgcgga catcgaagcc gtatttcgcg gttattcgga agaactcaaa atcaaagcca
     5341 gtgaacttat tcatccggta cgcattgcga tttccggaat gactcttgga ccgggattat
     5401 ttgaactgct tgaagtgatc ggtaaggaag ccgtcatacg tcgtatgcgt cgcgctattg
     5461 acgtgtatga tcacgtcgtc ggtgaagcgc aatcggtaaa ataagaaatg gttatgattc
     5521 gttaatgtaa aaccgcattt tgtttaacaa tttttttatt tcagtagccc ggcccatagg
     5581 tcgggttatt tgatttaatg cacacgcatg agaccttgtc tcatgctaag cgaaaaaatg
     5641 ttatcgttta gcttttcaga tacgtacatt gatgcggttt ttaattccta aaaaattatg
     5701 gaaaaatcaa tagcacccga acatccgatt cacgtttctt cacaaacgga catcaaaact
     5761 gccgcactca ccgtgcatga caataaaatc gccatgcgct ggtcgttgtt tatcggattt
     5821 ggaatgtttg cgatgaagat atacgcttac tttattaccg gttccgctgc gattctttcg
     5881 gatgcggcag agtcggtagt tcacgttctg gcggtatcct ttgccgcgta tagcctctgg
     5941 ttgagtacgc aaccacccga taaaaatcat ccctacgggc acgatcgtat cagttttttt
     6001 tcggccggct ttgaaggtgc aatgatcgtg atggcggcgc tttatatcat ctatgaatcg
     6061 gtgaagaagt ggattcgcgg attggaaatt cataatattg agtcgggtac gatgttcgtc
     6121 gtgatagcca cagccgtcaa cgcggccttg ggttggtatc tcgttcacaa agggaagaag
     6181 aatcactcgc tgattttaat agccaatggt aaacacgttc tgaccgacag ttggaccagt
     6241 ttcggggtga ttttcggatt agtgttgacg atggtgacgg gatggttacc gtttgatccg
     6301 atcgtggcta ttttagttgc gctcaatatt ttatggtcgg gaggcaaatt gatacgtcag
     6361 gcgtttggcg gattgatgga tgaagcggat ccggagacgc aaaccaaatt agaatctatc
     6421 ctgcaagcgg aaacacaaaa atacggaatt cgttttcatg atttaaaaca tcgcagttca
     6481 ggaaatcgga tcatcattga gtttcatctt ttgtttcatg acagcttgac gatcgtgcag
     6541 gcacatgccc aagccacagc tatcgaaaaa gttatcgccg atacatttcc gatagaaaca
     6601 gaaatcacat cgcacctgga acctatcgaa ggacacaacg aagcgcatat cacaaataca
     6661 agaatgcgaa atggtaaaaa cgaataagga gagaaccgat cagggttcga tctgagtgtg
     6721 taagccgatt tcctcaagta cgctgctgac ccgcaaacat tcccctaaat caccttcata
     6781 gacgacggct tttcctcggg tatgcacttc ccatgcgata gcttcggctt tttgaacggt
     6841 acacccgatg gctttgatca cctgagcgat cacttcatca aaggtatgga tgtcatcatt
     6901 gaaaaggatg acacgtaaag gatgatctaa ggtagttgat tcggaaactt catgttccgt
     6961 ttgggtggcg attgccgtaa tgggtttcat aatgaggaat ctattaaaaa actaatcatc
     7021 atccaataag aaatccggat tcatcatccg gatttcttga agaacgattt ttaaataagc
     7081 gcttacaaat taaattccgg tctgaaatct ttgatggtcg cttgcttttt catgctttcg
     7141 acccagtcgc gataagcgcg ttgttgtttt gtgctttgca attggcgacg cagatcattc
     7201 cgtgcatcgc ggaagagcga ttcattgata tcatcacgtg tagcgacaaa tgcaatataa
     7261 gcgccacgat tgcctttgat ggcttcactc agcgtaccca cggtggtatt aaataatacg
     7321 ccgttgattt tagcgtcacg accgacaccg atgatggagt tattcatcgt aaaattagtc
     7381 agatcgcgta cggtcaatgt agaatcaagc gccttcaagt cagccagcga gttgattttg
     7441 gaacgtaact cctgagcttt ttgataggcc atcgtcatgc gtttttcctc catcaccgta
     7501 ttgcgaacgc gctctttgac gacttcgaaa ggttgaatac ctttatcggt gcgtgaaacg
     7561 attgcaaaaa ttgtaacgcc gcgtccggtt tgatgcgggc gatggaccgc accggctttt
     7621 tcggtaaagg caaaacgtgt cacgtttttc atccgttcgg caaaaccggc aacgataccg
     7681 ttttcattat tgacgatgtc cagcgtcgtg tcggacgtta ggttaaagcg tgatgcgtat
     7741 ttttcgagcg ctttgtcaaa tccgttatct ttggctgttt cataaaatgc gttggcattc
     7801 tcacgggctg tctcgatcgt cgtctggctg gcttccatac gaataagaat atggcgcgcc
     7861 aagacactat cacggtcttc ggcttcgccg gcttttttcg cacgtgcttt tttatctttg
     7921 acaaaacgcg tggagtcaat tttaataatg tgataaccga aagacgtaat tacagggccg
     7981 atcacatcac cttttttggc gttgaaacag gcttcttcaa attcacgaac catcgctcct
     8041 ttggtaaacc aaccgagcga accgccttgt tgattggccg cacgatcaac cgagttgagt
     8101 ttggctaatt cggcaaaatc tttaccttcg agaacttgtt tttttacttc attgacacgt
     8161 tcgatgactt cagcggaatc ctctttggta gcttccgtac tgaacgaaac aaaagcgagt
     8221 cgcgcttttt cttcttgttt gtaatcatct ttgtggttgt cgtaatacga ctgcgcttcc
     8281 gcatcggtca cttgtacgct ggatgcggga atatctgcgg ggctaaaaaa tacaatctgg
     8341 ccgttcagct tgatattgcg acgggcatac tcttgacgca attcgctttc gctgatacga
     8401 ggtgaggtag caatcatagc ctgaagctta ttgtacggca tttgcatacg cagcatttgt
     8461 tcgacctgcg cccattcttt ggcgtactga ggattattga gcgcttgctg gtatttattc
     8521 atatcaaaat taccgtcggt caaaaacgca ggcgtgtttt tgataaactc cggaggattg
     8581 ttgcgcagtt caaataccac ttcggtatcg ccgacagcaa catggttttc tttcatgtat
     8641 tcgcgcagca atttttcttc gacgatttgg ttccaaatct gatcgctgag ttgctccatc
     8701 tccgcgtcgg tcaacgcttc gccgtctttt tgggcgcggg cattattgat ctggttgtta
     8761 tacacggtag caaagtcggc gtagcgaata tcttcaccgt ttattttgcc gatcgtgccg
     8821 cgggatttat tggcgatgtc agtgtagtta gcgccccatt cgatcacgat cagagcgata
     8881 aaaaatacga tcaacgcata gagtaaccag tgcgtttttt cacgaatcca cgtcatcata
     8941 cgagcatttc tcctgcaata actttatagt caatttgata attatttacg gcttcatatt
     9001 aaagctgggc aagataggcc aaaacctcaa taaatgcaat tctttttcct gtttagagtt
     9061 gttttataaa gccgatgaaa aaccgggaaa aaaacacgga gaaatatgaa aatatctctc
     9121 cgtttagggt taggaaatga gtacgtagaa ctacttgtat tgtgcaatcg cccaataagc
     9181 tgttgattct gaggtcttat agcggtattt tagagtgttt aaggcatatc ttttggtttt
     9241 ctcaacatac atccattaca catgcgctac ttgtttcatt tccgtaatca ttggtttcga
     9301 catatccgcc gttggattat tttgtcatcg gttttttgcg ccgttatgct taataacgac
     9361 ggatggactc agcaagaagg cattgaagtt ttaaaaaccg ccattcaggg ggtggcgtat
     9421 gattccggta aagtcaatct gatgcacgat ctggcattcc aatatctgga agtagatctc
     9481 gactcggccg aatattatat ccgcaaatcc gaagagctca gccgcgtttt taaatacaaa
     9541 tttggtgaag tcaaagccgt caattatctc ggttacgttt ttgagcagcg aggccgtgta
     9601 gatgatgcta agcgtctcta tggcgatgcg ttgaaaatgg cgatgaataa taatgatacg
     9661 gtcggtttgt cgcgagctta caatagtctt gccggcattg ccatgatcgg cggtaaatac
     9721 cgtgaagcgc tcggttatta cgaaaacatg ttgggcgtct atcagggaag agcggattta
     9781 caaagtcagt ctgtcgcata caataatatc ggcaacgtgt atgtagaaat gggtgatcat
     9841 gagcaggctt tggaatatta tttcaaatcg ctcaaaattg cccaagaagc cggcgaccaa
     9901 aaaagcctag gcacatcgta tatcaatctc ggtaatgttt tcctggaaca atccaaatat
     9961 gtcgaagcgt tagagtatta tcagaagtcg ctggaaatac aggaaaaaat aggttcccag
    10021 tccggtacgg cgctttgttt tctaaatatc ggttctgtct acgaaaagca agaacgtcta
    10081 gatagcgctc tacatttata tcggaaagca tacgggttgt ataagcaact tgacaataaa
    10141 tccggcgtgg catggacatt ggtaagcctt ggcgacgtat acaaccgaaa gggaaattat
    10201 gcggaggcgg cgacatacct gcgccaatca ctcgaaatgg cgaagtttgt gaaagatctt
    10261 accgccgaag gtaatgcgta tgtcagcttg gctcaaaatt ataaaaagct cagaatgtac
    10321 tatatggcct tgcaatacgc aggaatgggg catcagattt accttgcatc gggtaatcgc
    10381 aatcgcattc gtgatgtgcg ccttgtgttt tcagaagtct atgaagcgat ggggtatttg
    10441 ccgcaggcat tgacttggta caagggttat atcgcaatca aagattcgtt acagagcgta
    10501 ggagcaaacc gtaaaacagc ggaacttcag ttgctctatg attcaaaacg caaagatcag
    10561 cagatcgctc ttcttcagcg cgacagtacg atcaaatcgc ttgaaatcag tcaaagtaaa
    10621 attgtgcgcc attcactgat cggaggattt ttggcgttga tcgtggtttt ggtgttactg
    10681 tggcaacgct atcgtgtcaa acgtcaatcg gaagaattac tgaataacaa aaatgccgaa
    10741 ttgactaaaa taaacagcga tctggaggaa gcgctccaca atgtcaaaga gttacgcggc
    10801 atgtttccga tctgcgccaa ttgtaaaaaa gtacgcgacg accacggatt ttggagtcaa
    10861 atcgagacgt atctccagaa gcataccgat gcggagttta cccacagtgt ctgccccgaa
    10921 tgccttaata cactctatcc tgaattcgtc aaacgcgcca aagaagcgga atagtgtcga
    10981 tcgttattcg atttggaatg aatttctcct ttttcttttg aatatcctgc ctgatttatc
    11041 tatccctgat ttgttttcat aatgctttaa gatcggcgtt tatgcgcaaa gtttatttct
    11101 tcttctgatg tatcgcgtgt ttggtagtta ataaatacgg tgtttagttt gctttttatt
    11161 taaaagtaag acaacgcaat cttgtttaat tcaagtcctt ttgaataagg gaatagaaaa
    11221 gtatcaacta aaaccttaga ttttggaaaa gctatagacg atgaggttgt cagtaaattc
    11281 gtaattatca gttacgaaaa ataattcttg acaaaataat caataatgaa tatatattaa
    11341 tcatcaattg atactaagtc tcaatttcaa ttaataataa acaattttat attataatta
    11401 cataaaaaca tgataattat cattgagaac cgttctcagt tattagataa taggaaaata
    11461 aaatttgttt tatttatttt taatattgag actgaatctc aatttcaatt ataactcttt
    11521 aaatattaac cgaggattgt tttatgaaaa cgtttttttt gaccctgttt ttgacatggt
    11581 gtggatttaa tacattaacc gctcaagatc gtattttcaa ctacacgtac caaagcaatg
    11641 tactcaatgc cggccaacgt gaagtcgaac cttgggtaac cttcaaatgg ggccgcgaaa
    11701 aattttacag agcatttgaa aatcgcatcg aatatgaact cggcttcgcc aaaaatcttc
    11761 aaatggcatt ttatatcaac gcaaaaacgt cggcggctca gaccaatgac tcgacaatag
    11821 aaaatgcaac cgaactgagt ttttccaatg aatggaaata caaagtattt gacgccgtgg
    11881 ccgatcccgt aggatttgca ttgtatggcg aatacggtat cgggcgcaat gagattgaaa
    11941 tcgaaggcaa gttgatcttt gataaacgta tcgggaattc cattctggcg cttaatcttg
    12001 tcggtgaact ggaatttgaa aaagaagcag aacacggtga gatcgaaaat gaaaaagaaa
    12061 ctgtttacga attggatctc ggatatagtt accgcgttcg caacggatta tacttgggta
    12121 ttgaagcccg caatcataat gaagtggtcg aaggcaaatg ggaacattcc gcgttattta
    12181 tgggtcccac gctgagctac acaatggaaa atttttgggt caatctgacc atcttgccgc
    12241 aggttaaagc gctaaaaggc gccaccaaaa acggtttggt attggatgaa catgagcgcc
    12301 tcgaaacgcg cctgattttc tcttacatat tctgagcaca gccatgaaaa aatacttttt
    12361 ttgcctttcg ctgctgctta tgtctagttg tgcaccggcg ctctatgtac cgagcgccgc
    12421 tacaacgagt gatcccgccg aacttacggt tttaaacgaa gggcgcgcga tgtatgtgca
    12481 gcattgcgga agttgccaca gcctttttgt accgtcggat ttttccgatg aagcatggga
    12541 ggcgcattta gatcaaatgc agacgcgggc taaaatttcc gatcatcaaa aagaacggat
    12601 tttaaagtat cttaccagct ataaaaaacc ggagaaaaaa taatgagtac gcatcgtttt
    12661 gtggcgatca attatatcac ttgcaacgat aattatcgtg aacgatttga agaacttttc
    12721 agttcacgcg cgcatgccat tgaccgcatg ccgggatttg tggatatgca ggttctcaaa
    12781 cccgatcaac cggacgagcc gtatctgatc gtgagtcatt gggatagcaa ggaagcgttt
    12841 gatacctgga cccaatcgcc ggaattcatc gaaggccaca agcgcggatt tgccgatgta
    12901 cgtgctgcca aagcgcgcgg agaagtcgca cctatggagt ccaaatttaa aacctatgcg
    12961 gtgatcgcgc gatgaatttt aagaatcgct ggccgtgggc caaaaaactc ggtatcatcg
    13021 gttttttgtt ttttttaatc aaaggcgtat tgtggctcat tgtgcccatg gccacagtat
    13081 gggtgtgcaa ctagtatcac aaaaatgacg gatttaccga atggctgatt gttttcatcg
    13141 catcgtccaa caaaacgaga tgggctacgt ctccgtctgc gaaatgtgcg aacgcataca
    13201 ggttggtttt ggtacggcgc tagctaattt tacacggcgg gaattttccg atttcagaga
    13261 acgtattgtt cgtgagtgga tggctctgaa atcggaacct aatccgcgcg aaaaaaatat
    13321 tttcatcgaa acttcttcca gagcatggca tttggcgctc acgggttcgg agctttccaa
    13381 actgacggat ttattggaaa aagcggatac ggtactgagt ttgacaagtc aacgcgcgac
    13441 gtattataac tgagggatca acgatgaaat ccgattgttg caaaaactat aagaaaaaag
    13501 ggaaggcttg ccgcgaatgt cccgtactgg ccaagctgcg ggagcgtcat gctgatctgc
    13561 gtcgcaaaga agtattcgaa taccttcaaa atctcaaaag aaaccgtttc gtatgcgaag
    13621 tatcgtatga accttaattc ctgatagtat ataaaagtca aaacacaccg catttagttt
    13681 ccgtcttatc cctcacacaa tttccgaaaa aatatttacc ctccggttga catcctgaag
    13741 catactttta aaagttattg gtgcttatca tttcaccttg gtgatcacgt actgttttta
    13801 tttttttaaa tctgaagatt ttcataagtg ttttttttat aaaaacatat tattatttat
    13861 ctttttcgtg ttttacttac actgtaacaa ttcttaacat gaccaccgat ctaaaaaaat
    13921 ttctcagccg gtatgcgggc ttctcggaag cggaattgga aaacattgcc ggaaagttca
    13981 aaagccagaa gcttcgaaag aatagttata tgctgcggca aggtgaagtg tgcagaaata
    14041 tcgtgtttgt tcaaaaaggc tgtttgcgtt tattttatcg aaaagacgac gtagatgtat
    14101 ccgtatggtt ttcttttgcg caatcctccg cgatcgagat atacagtttt atcagtgaaa
    14161 atccttccga ctattacttg caagccatcg aggagagtga agtactctac ctttccaaga
    14221 ccgaactcaa aaaactctac gcgcgtcatc cgaacatgca acagatgatg cgtaactttt
    14281 gggaagatgt cattcttaac ctgatcaaac gatttacggc gctacagacc gattcggccg
    14341 aaaaaagata tctggacctg cttggcaaac cggacttttt aggaacgatc ccgcaaaaat
    14401 acctcgcttc attcatcggt gttaccccca cctcgctaag ccgtatacga cggcaaatta
    14461 cccgaaaaca taaattgtcc taagacaact tttttagatc aaaaaggttg tagtttaata
    14521 ttgtcatttt gagcggacag gattatgtcg tttcagacat acgggcataa aatttttttg
    14581 atgccgctca aggtgcatcc catgcgatag gacaaaacag gtattttaaa cctataaccg
    14641 aaggagattc gtatgtataa cttttcgtat ttcaaagaaa aagaacggca agtaatacgg
    14701 gagtttatgg aaggtaatcc gtttgctttt ttgacaggta gttttttgtc cggagtacaa
    14761 gtgggaacgc agattccgat tttgttcgaa gagcgcgacg gagagttgta tttgcagggg
    14821 cacatcatgc gacatacgga ccaccacaag gctttggtcg aaaatccgaa tgccttggtt
    14881 gtttttacgg ggcccagtac gtacgtgagc gcctcgtggt atagtaatcc tcacatcggt
    14941 tcgacatgga attatatgag cgtctatatc agtggaaaga tgaagttcat gtcgtcggat
    15001 gagttgatcc ggttcatgcg aaagttgaca ctgaaatttg aaaaaggcaa cacgcagtca
    15061 cagactattt atgataatct gcccgaatcc ttcttgaata aaatgatgcc ggctattgcc
    15121 ggatttgaga tcaaagcgga tacgttggat aacgtgttta agctgagcca aaaccgggat
    15181 gaaaaaagct atatgaatat catttccaaa cttgaagcgc aaggcggcaa tagtgcgctt
    15241 atcgctcaag aaatgatcaa aagaaaatcg gaattattcc ctcccggcgt tgcatgggat
    15301 gcctcgaaat ttgattcgta atctttctaa caaacaataa aacaccataa agtgatggag
    15361 ataaaaaaac tcactgccga tgaagtgtcg gacttcaaaa gattaattga aatcttcaaa
    15421 gatgtgtttg aaaatgatgt ggatattcct ccggtggatc atttgcgacg gttgctttcc
    15481 aatccggact ttatggtgtt tgtcgccctg agcggtggca atgtggcggg cggcctgacg
    15541 atctatgttt tgcaccgata ctacagtgtg aaaccggaag cgtatatcta tgatgtgggc
    15601 atatcgccgg agttccaaag aaaaggttta gggaaagctt tgatggcgga ggtatgccgt
    15661 tactgtcaag cgaacggatt tgaaaacgca tacgtggaag ctgaacatga tgatgttgaa
    15721 gctgtccggt tttacagagc cacggcttac aatcatgaag tgcatgccgt tcatttcacg
    15781 tatgtttggc ggtaatatga tgttagaata atgagcgttt tgtttctcaa gcttttacga
    15841 tccggtatgt gaaataaact tgagatataa ggttgagctc tgcagctgtt attattttaa
    15901 aaaatgtatc tctgatgcag cgctaaacac tcatcgaaga attgtcattg atttgaaaca
    15961 gagctaagtc tttatttcac aacgaaaatt atttttaaaa tttccatatt ttctcttgac
    16021 atgcgaccct ctttcgtata tatttgcaag cctttttaga tactcaacct gcctcaggtt
    16081 tccgcggaag cggagttcgg atgcgcaggc aatgataaaa aacccccagg ttttctgtca
    16141 aataagagta aaaacagagt ttgttacagt ataactacta aataacagtt tagcaggaag
    16201 tgcattaatg ccaacgatcc aacagcttat tcgcaaaggc agacaatcca agacttggaa
    16261 aacgaagtcg cccgctttgc aggaatgccc ccagcgccgc ggcgtctgta cgcgcgtcta
    16321 cacgacaacc cctaagaagc ctaattcggc cctccgtaag gtagccaagg tgcgtctctc
    16381 caatggtatc gaagtgatcg cctatattcc cggtgaaggc cataacctcc aggagcactc
    16441 gatcgtgctt atccgtggtg gtcgtgtgaa agatctcccg ggtgtgcgtt atcacatcgt
    16501 tcgcggtgtt ttggatacgc aaggtgttca agatcgtaaa caaggtcgtt ccaaatacgg
    16561 caccaaaaaa ggtaaagaag gcgccgctaa gaagtaatct tagataaaaa agtttgatat
    16621 agaggaaata tgtcgagaag aaaaagaagt cccaagcgcc gcattccggc ggatcccaaa
    16681 ttcaacagcg tcttagtcac gaaaatgatc aatgtgatca tgtggcaagg taaacgcggc
    16741 gttgccgaat ccatcctgta tgatgcgatg gaaactgtcg aaaagaagac cggtaaaaac
    16801 ggtttggaaa tcttggaaaa agcgatcaaa aacgttgctc cgcttttaga agtacgttcc
    16861 cgacgcgtcg gtggcgccaa ctatcaagtg cctgtcgaag tgcgtgccga acgtcgtcaa
    16921 acgttggcct tgcgttggtt gatcggctac tctcgtgaac gcgccggcaa aagcatggct
    16981 tctaaattgg cggatgaaat tttagccgcg tccaataatg aaggcgcggc cgtcaaaaaa
    17041 cgtgaagatg tgcataagat ggctgaagcg aacaaagcat ttgcgcactt ccgctggtaa
    17101 tagtcagaaa tagaaaatac gaataacagg aacttataaa taaaatgccc agatcaattt
    17161 cgttagaaaa aacacgcaat atcggcatca tggctcacat tgatgcgggt aagaccacga
    17221 cgacagagcg tatcctttat tatacgggcg ttctgcaccg tatcggtgaa gtgcacgatg
    17281 gtgcggctac catggactgg atggagcagg aaaaagagcg tggtatcacg atcacgtctg
    17341 cggcaactac atgcttctgg aaaaatcacc gtattaatat tatagatacg cccggccacg
    17401 ttgacttcac tgtcgaagta gagcgctcgt tgcgcgtatt agatggtgcg attgcgttgt
    17461 tttgtgcagt cggcggcgtt gagccccaat cggaaacggt atggcgtcag gcggataaat
    17521 acggcgttcc ccgtatcgcg tttgtcaata aaatggatcg tatcggcgca gacttcttca
    17581 acgtcgtaaa gatgatgaaa gaacgtctcg gtgcgcatcc agtgccgatc accttcccga
    17641 tgggcgaagg cgatatgttt acgggtgtga ttgatttgat cgatatgaaa gcgatcgtat
    17701 ttgacgagtc ttcccaaggt atgcgctggg atgtgattga aatccccgcc gatctgaaag
    17761 aaaaagcgga caatgcgcgt cagttcatgc ttgagcagat tgcggattac gatgaagtcc
    17821 tgatggaaaa atatctcaat ggtcaggata ttacaaaaca agaaattcaa aaagctattc
    17881 acgccggtac tttgcaatgc aaaatcactc cagtactttg cggttcatct ttcaaaaaca
    17941 aaggcgtaca gcgtttactg gatgccgttc tcgaatactt gcccaatccg cttgaaggcg
    18001 gtaatctcaa aggtcataat ccggatgacc acacgcaagt gattgaacga cgcattgatg
    18061 acagtgaaaa attttgcgct ttggcattta aaattatgac ggatccttat gtcggtaaat
    18121 tgacattcat ccgtatttat tcgggtatgc tcaaagccgg ttcgtatgca ctcaacgtta
    18181 ccagtggtaa aaaagaacgt atcggtcgtg tgcttcagat gcacgccaac catcgtgaag
    18241 atattgacgc gtgctatgcg ggcgatatct gcgcggctgt cggtttgaag tttacaaaaa
    18301 ccggcgatac gctcagcgcg gaagattctc cgattctttt agaaaaaatg gaattccctg
    18361 agccggtgat cgaagttgcg atcgaaccca agaccaaagc cgatcaggaa aaactctccg
    18421 aagcgcttag caaacttgcc gaagaagatc cgacattccg tgtttcgacc aacgaagaaa
    18481 cgggtcagac gatcatcaaa ggtatgggcg aattgcacct tgaaatcatc gtggatcgta
    18541 tgttccgcga gtttaaggtc gaagcgaatg tcggtaaacc gcaagtagct tataaagaag
    18601 gtatcactaa aaaagttgaa gccgaaggta agtttgttcg tcagtccggt ggtcgcggtc
    18661 agtatggcca cgtctggatc gaagtcgaac cgaatgaagc tggtaaaggt tttgaattta
    18721 ccaacggtat tgtgggaggt gtggttccta aagaatacat caagcctacc gaagaaggta
    18781 tcaaagaagc gatgaagaac ggtgtgattg ccggcttccc gctcgaagac gtcaaagtta
    18841 aattattcga cggttcatat cacgatgtgg attccagcga aatggcgttt aaaatcgccg
    18901 gctcgatggc catccaggcg gccgctaaaa aagccggccc gatcatcctt gagccgatca
    18961 tgtttgtgga agtagttgta cccgaagatt atctcggcgc cgtaatgggt gatcttaact
    19021 cccgtcgcgg tcagatcatg ggtatgaatc aacgtaaaga tgcacaagtc gtgcacgcgc
    19081 acgtaccgct cgccgaaatg ttcggttatg ctacggcgtt gcgttccgcg acacaaggtc
    19141 gtgcgattta ttcgatgcac ttccatcact atgatccggt gccgcgtgct gtcgaagcgc
    19201 agttgcttga aaaagtaaaa gcttaatata aatagatatc caccaggaga cttaaccatg
    19261 gcaaaagaga aatttaaccg ttcaaagccg cacgtgaatg tgggcacgat
//
LOCUS       DAOQNQ010000034        19310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_998_length_19310_cov_5.982290, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000034 DAOQNQ010000000
VERSION     DAOQNQ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19310
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_998_length_19310_cov_5.982290"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2593
                     /locus_tag="PK754_03940"
     CDS             <1..2593
                     /locus_tag="PK754_03940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB08685.1"
                     /translation="AWMTRSGWLLYPTGKGLAAVNPRLLSRVSPSPLVVIERMLAEQK
                     LIGLGEKIELTPGHRRIEFTFTGLSFTAPENNTFMYRLIGFDKEWIQAEKTRHAVYTN
                     VPPGAYRFEAKASNSDGIWSSQPATVLFSIDPYFFETVWFYSLSGVFLLYLGFTYYRS
                     RVRELHERQEELTRLVQERTRNLREEKEKTEHALRESEEAKREIEKSQQVIQSQSQKL
                     IELDKVKTNFFTNISHEFRTPLTLIIGPLEDVLRSATLSADQRVSFELMLRQAKQLLG
                     LINQLLEAARLESNRVQFHPSLQPLVPFVRELTSMFESAAQRKGIHLSLETYDEKIQL
                     YYDLHAMERVLTNLISNAIKFTEQGFIKITIKHSEHTHRPSVVIFVEDSGRGIPQEHL
                     DHIFQRFYQADGVEADQLGSGIGLSLVHDYVEMHRGLITVESTVGKGTRFMITLFTGE
                     EHLSGTITPHAEIKTETEFIYARSIAALSSEHNIAPEISAPDPMERQTADKPKVLIAD
                     DNADIRLYLYNLLRDEYRMIMAHDGAAALDLLQREFPDMVLSDVMMPNMDGMELCRRI
                     KSDERTRHIPVILLTARADESSKVESLNLGADDFVAKPFSANELKARMRNLLGIHAMK
                     LQLIEQEKMASLGHLARGVAHEINNPLSFVTGNLSVLESRLAKLHPDENIKPIVTDIM
                     SGTKRIAAIVQHLREFTRLDEAERKYVDLSDMVDNILTLLAGEMQSDIRIEKNIAQVP
                     MIDCFPRDLNQAIAQILMNATYYVRERNRIENRSLGHITIHLNKADEKYVELIIENDG
                     FPIAPEIREHIFEPFFTTKPIGKGTGLGLSAVYGIIRRHHGQIRCVSEPFQPVRFIIM
                     LPFAIPS"
     gene            2791..4059
                     /locus_tag="PK754_03945"
     CDS             2791..4059
                     /locus_tag="PK754_03945"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNB08686.1"
                     /translation="MKSILHSITRILTLFYILAFINQAWATDGYFSHGYGTKQKSMGG
                     VSTALTFNAFSAITNPANAVYIQNNWGLGIAFFNPNRQYAVSGSPSGFPNTFPLTPGT
                     VKSKSTGFFIPSLSYNRLINEKSAFNFAIFGNGGMNTDYKTNTFNTTKPTGVNLSQLF
                     VNTSYAYKVTDNHAFGVTAIFGYQWFEAKGLTAFSGFSRYNTKLTDNKSDKAYGYGAR
                     LGYSGQITPQLRIGASYQTRIWMTEFNDYKGLFAEKGDFDVPATWNAGLAWDINSAWT
                     LAADLTQIYYSSVKSVGNPMKPADFQNDILLGDKKGAGFGWNDMTVYKIGCEYRHGND
                     WSARVGYNYGKQPIPTSEVMFNILAPGVIEHHLTFGASKKIGSKYEFSLAVMRGFSNE
                     VKGANPMEAPGQQAIALKMNQWEFEIGLGF"
     gene            4081..6273
                     /locus_tag="PK754_03950"
     CDS             4081..6273
                     /locus_tag="PK754_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNB08687.1"
                     /translation="MLSRRRFLKIGAGFTAGATIAAGAAHTLADRLAALEKPSGQVEV
                     IPTYCDICFWKCGVLAHVKDGQLWKLEGNPEDPLSRGRLCPRGTGGVGAHYDPDRLRT
                     PLIRRGEKGAEEWTSVTWDEALDFIAEKMKKIKAEYGPESVALFSHGIGGNFFKHTIK
                     AYGSPNIAAPSFAQCRGPRDVGFRLTFGEDVSSPERTDIRNAKCLVLIGSHLGENMHN
                     TQVQEFAEAVDHHATIIVVDPRYSVAASKAQHYLPIKPGTDLALILAWMHVIVKENLY
                     DKAYVEQYGFGFDAFVAALETYTPEWAYPETGIDPETIRTTARVMAQHKPATLVHPGR
                     HVTWYGNDAQRSRGIALLNALLGSWGSKGGFYTPVSMDVPAFPYPSYPKSDKGKVDNP
                     GHKYPFAHETITTGIREATLTGKPYPIKGWFVYATNLLHALPNEEETIKAIQNLDLMV
                     VIDVIPSEIAGWADVILPESVYLERHDDLNVEWFRDPFVALRQPVVSAPHDQKPNWWM
                     AKRLAEKLGLGHYYPWTHIESYFETRLRQAGLSYDTLKKNGILLGEKKPVYFDEGVPA
                     EFPTPSGKIEFYSSQLAEAGFEPVPKYVAPPQPPPGSFRLLFGRAPVHSFSRTHTNPV
                     LMDMMNENELWVNADMALRYGLKSGDYVRLKNQDGVVSTKIKIKATERIRTDCVYMVH
                     GFGHNAKALKRAFGRGASDAQLITRYDTDPLMGGTGMNNNFVTLEMEA"
     gene            6276..6815
                     /locus_tag="PK754_03955"
     CDS             6276..6815
                     /locus_tag="PK754_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNB08688.1"
                     /translation="MARLAMVIDTRKCVGCMDCVVACKTENQVPEGYNRDWITTEAKG
                     KFPDVRLEIRSERCNHCSNPPCVTCCPTGASHVHDYGGVVLVDHDVCIGCKACVASCP
                     YDARFVHPEGYVDKCTFCIHRVEKGLEPACVSVCPTHCMYFGDLDDPNSKVSQLLQKR
                     AYHTNLPEAGTQPNIFYLT"
     gene            6823..7764
                     /gene="nrfD"
                     /locus_tag="PK754_03960"
     CDS             6823..7764
                     /gene="nrfD"
                     /locus_tag="PK754_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNB08689.1"
                     /translation="MNELTHTRMNPLIDPALHIWGWEIPVYLFLGGLVAGFMILSGYF
                     TLKGHHKHDHFSSFYLPHVSLALLSLGMFSLFLDLEHKLYVWRLYTTFEITSPMSWGS
                     WILLLVYPSLLLNTLVRIPEAFSNRIHLFDKFSERIHAHPYLIKNIGVLNMIVGAILG
                     IYTGVLLSGLAARPLWNSSMLWILFLTSGISAAAAFVHLVTTDSVEREISAKADNAFL
                     IFELFVITLFIVGMLSSAQAQQTAARLILNGPYASVFWVFVVGCGIIIPLIIQLLAVN
                     HKIQHTPIAPIMVMLGGLILRFVIVYAGQASHWTIVH"
     gene            7782..8339
                     /locus_tag="PK754_03965"
     CDS             7782..8339
                     /locus_tag="PK754_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNB08690.1"
                     /translation="MASSEEHFNEHRAVKPPQPYWNPYLSGIGLGLVLLASYLIMGRG
                     LGASGAFTTTTAVAVNAVAPEYAHQNTFYSEYLGDGTRSPLKDWLVFEVLGVFVGGLV
                     SGLLAGRIKKSVEKGPRISVRSRLLFAFAGGMLMGIGAKLARGCTSGQALTGGAVLNL
                     GSWAFMLMVFAGAYGLAYFLRRQWT"
     gene            8336..8935
                     /locus_tag="PK754_03970"
     CDS             8336..8935
                     /locus_tag="PK754_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNB08691.1"
                     /translation="MNAPFFKYGLFGDEASLIVAFIIGIGFGFFLERAGFGSGVKLAA
                     QFYFYDMSVLKVMFSAIVTAMLGVYWFSWFGLLDLDLIYINPTFLLPQIVGGLLLGFG
                     FVIGGYCPGTSVVACSTGRVDAMVYVAGIFGGIFTFGFIFPYLESFYTATPMGAITLP
                     QWLNLPYGTVVFLVVVMAIGAFAAAEWGERKMAPRGGAS"
     gene            8932..9585
                     /locus_tag="PK754_03975"
     CDS             8932..9585
                     /locus_tag="PK754_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB08692.1"
                     /translation="MKNLWRDLTLTQKFGVVALMLGLLALIGGTPQRGSFYTIDSKAM
                     LMDVAKETDHIAVETLADWIIQGRSDYRLLDIRDAKAFETYHIPSAESVPLAELHQAE
                     LRRNEKIIFYSDGGIHASQAWFVLKARGYNGVYMLRGGLEEWQDRILYPHKPSTTDTE
                     SLRQFEKSAAVAKFFGGETQGEQSTAAVKSATTPLPKLPAGAKAAAPKTGKKKKEGC"
     gene            9852..11879
                     /locus_tag="PK754_03980"
     CDS             9852..11879
                     /locus_tag="PK754_03980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HNB08693.1"
                     /translation="MRRLSNFILIMVLSLPLYSQDTLTLKNISYQPGQKLFVGLENED
                     LGAPLRVQLLNADGKPVSNRIVLFEIIERPSKSKGEKLGQAEAITNAEGMAETSFRLG
                     DHAGDYIIIARTTGMTGDTPHIIVRAQRTMWWMFLIFGLIGGLGIFLYGMNLGSGGLQ
                     KVAGDRLRTILGALTSNRFLGLLVGIAVTAIVQSSSATSVMVVGLVSSALMTLQQAIG
                     VLMGAHIGTTITVQLIAFNVSDYSLLLVGAGFLMTIATKRKFYIYMGEIILGFGFIFF
                     GMAVMSQAINPLKSMPAFIAWLIEFGNSPILGILASTVFTAIIQSSGATIGLCVVMAG
                     EGLLSLQSAMPLVFGANIGTCATALLSTIGATTDGKRTALAHTAFSVIGVFLFTPFLT
                     PFESLIQSITSWMGTEAVARQIANGHMIFNIMTAMILIGFVPLMTQLITKIIPEKAEG
                     DAFKPKYLNNAYLETPDIALQNAQREEVRLVTIVRAMYDNTMNLFSSENEEKVAKVRE
                     QLKQVNFLEGEIRRYLTRLSQKSISLTQSRRLMRLLLTIDDLRHMAERLGDSIANLAE
                     RYADNHVAFSPEGLEELKRFYGMIHERLDAVLTALEKEDAVMAEHIIKNKHMVSEEEV
                     RLRQLHMTRLQGGNEQLFVSSQYHFDLLTALQRVHIFCVKMAHEIVEQHNL"
     gene            complement(12091..13869)
                     /locus_tag="PK754_03985"
     CDS             complement(12091..13869)
                     /locus_tag="PK754_03985"
                     /inference="COORDINATES: protein motif:HMM:NF039346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB08694.1"
                     /translation="MTILIVDDERTSVDALSLALGQSGYSVVTALNGKAALTILAGQS
                     PDLIISDIMMPELDGFGFIEAVRRQPRLASIPFLLMSGDKDPSLTAKAIQMGAIDFLP
                     KPINLNLLFLKIQALFRFVEAQRSSGILRSGSLVLGKIEELIASAEKEGIHGILTIQS
                     DHGVGSIEFNRGVCETIVCGELKDSEALETMLQWSSGSYYLARRPTELTESENKIPVS
                     VHSTKEGNAMKSEDFSKPIAIAEDIYFVGDRKESRILQINPFLRVYNNKVNFLVDPGP
                     TIDYTTISGKVGEVIGDVSKVNLYSLNHQDPDVASNTFFLKSANPKAICLTSEDTWRL
                     VSSFGINKERLILTDKLTNWQTNLPTGHTLQFIPTPYCHFRGAVMVYDIESRILFSGD
                     LFGGLTGTDNYGLWADESHWDGMRAFHELYMPANKAIQFAIDQIRKLDPAPVMIAPQH
                     GSLIRGELIREFLDRMYSLKVGVDLLGRTEDDQLLGQYLEVAKIVYKKAQAMVSNPDR
                     ILANFAGNAEIQELMTINDKDVVKIKKNASRALELFLYALSDHQSSRVSNQIKALMLQ
                     ETMIRQLPPPTFAIEEAMDLGMESFT"
     gene            complement(13899..19151)
                     /locus_tag="PK754_03990"
     CDS             complement(13899..19151)
                     /locus_tag="PK754_03990"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB08695.1"
                     /translation="MNKAGKSHTQIVLFLGSTAQASALTALTQWEVVSITDRNALRKV
                     TQIRSSSAILTTTYRRIASALVLKKKMMHAANLRVGFALPAAQGKRLVKEGVLKSDEV
                     IPLPFTKKNVLSFLSPTSHWPKQYDNSIASDLFLRAVESTDDAVIITDKDGHILYVNS
                     GFEKNSGYGRDEVLGKNPRILKSGYHEQAFYSGMWEKILSGSVFRNIQYNRRKDGSII
                     PVEHTISPVRNEIGEITHFIGIWKDISGKIRSENETRQAQDQLKQNEIKYQIIADNTF
                     DWEFWLDTDGSYIYSSPSCERITGYPPVSFTTDRDFLQRIVYAEDRAAFLAHQQDIQQ
                     RRLPGNLDFRIVCKGGVIKWIGHVCQPISDAAGVFRGVRGSNRDITERKLAELALRQK
                     ESQAESLLRLARQLERVAGFGEAAQAALTEVQRLVGYQRLWVYVFSEDRSEAEVLAEA
                     GMPRPFAPKRLDIKNDAMLSAVARGTGAVVIPDAAEDARTNKKIVQSTGVRTIVNIPV
                     HLSEGRLASIGMGTYDDEGVRVPDESALQYLTTLASHMSATIDRLLSQMVRQSAEDSL
                     RASEKRYRTLIDQAADGIFVATRDGQIEDVNPAGCRLMGYTVNELLKQPFYGLLDAEE
                     LIRQPLKIRQLEKGQSILSHRRLVRKDGTHVEVEISAKMLDDGRIQGIVRDISERRRV
                     EKHFQALIEHAPDGVVLINAEGKFQYISPSSARMFGYEMSELSDLEPAALTHPDDVER
                     VEQLIASVIENPDIDARAKYRFKHKNGSWKWIESTFTNLLQAPEVQAIVVNFKDVTER
                     HNAEEALLHSEQKYRTLAETSQDMVLLFENNGYIIYANPAVEQNLGYVQGALSGKMIS
                     TILPGNADYDAISYLTGGASHEIGAVSREIDFLHLNGEVIPFDVRTSKAVSSGTPVIV
                     CIGRDNRSRRKYENHIREQLRYARALNRMAASIMNSDGTAETLLREMTTICGETIDIE
                     RALLFDIRITGGEAVSIAKWIRPDFTDFAPPQEVYPTSVFASSISRLHSTRTCITSTV
                     DAPHACFVEEGSAEILHRVMKVQSLLWYPFSFREDGFFLLVVNDMIHPRQWQEEELNF
                     LDSVSREIELALLKHRLSSEREQAEVKLSETLREFETLVEAIPDAVFFKDGDGRWLIA
                     NPAALKLFELATYPWYGKTDQQLAEAFPALRLAYEGCVRTDEAAWRNGQRYQTIEIIE
                     KPGGDLLHFDTTKIPLLQENGARLGLVVIGRDITFHKKAKEIIQEQAMILDNARDAIF
                     MTDPEGRIIYWNSQCETLYGWEKANVIGHQATEVLGHTRATDAAARILFSGDAPWSGE
                     IEHYARDGRNVIVECRKSPVRDADSAVKSFLYIETDVTERKRLEKQYFRAQRMESIGR
                     MASGIAHDLNNILSPILMAVQMFKSKLPDDTSQRLIVMLESNIERAADLMRKLLMFSR
                     GAEGAHRVVDAKKTIEEIERILVSTFPKNIRIVCNVAKDLYPITADSTQLHQVFLNLC
                     VNARDAMPSGGDLTIHFENVIFDEAYVYKEHQAKPGPYVLFTVTDTGLGIPADIIDHI
                     FDPFFTTKDLEHGTGLGLSTVAAIIKGHGGFVSVYSEVGKGTQFKIYIPASAGFHTES
                     VESRIPESLLGRGELILIVDDEPAIRKILGTILTHNGYQIVEAEDGGKAMTVFAAQMA
                     SIRAVITDISMPHMDGHAFIRMLRSIREDVPIIAMSGLPENEKFIQKNGFKVQAFLMK
                     PYRAEKILQTLNDVLAPLRSAEQS"
BASE COUNT         4901 a         4808 c         4479 g         5122 t
ORIGIN      
        1 cgcatggatg acccgcagcg gatggcttct gtatccgacc ggtaaaggtc ttgctgccgt
       61 gaatccgcgt ttgttgtcgc gcgtttcccc gtcaccgctg gtcgtcattg agcgcatgct
      121 ggctgaacaa aaactcatcg gcttaggtga aaaaatcgaa ttgacgccgg gacaccgccg
      181 tattgaattt acatttaccg gtttaagttt tactgctccg gaaaacaaca catttatgta
      241 ccggcttatc ggttttgata aagaatggat ccaagccgaa aaaactcgtc atgccgtcta
      301 taccaatgta ccgcccggtg cataccgctt tgaggcgaag gcttccaata gcgacggcat
      361 ttggtcctcg caaccggcga cggtattatt ttcaattgat ccgtattttt ttgagaccgt
      421 atggttttac agtttatccg gtgtattcct tttgtatctc ggttttacgt attaccgttc
      481 gcgtgtgcgc gaactgcacg aacgtcagga agaactgacg cggcttgtac aggaacgcac
      541 acgtaatctg cgcgaggaaa aagaaaaaac cgaacacgcg ttgcgggaat ccgaggaagc
      601 taaacgcgag atcgaaaaat ctcagcaggt cattcagtcg caatcacaaa aattgatcga
      661 attggataaa gttaaaacca atttctttac caatatttct cacgaatttc gtaccccgct
      721 cacattgatc atcggacctt tggaagatgt attgcggtcc gccacgcttt cggccgatca
      781 acgcgtttca tttgaactca tgttacgtca agccaagcaa ctattgggtt tgatcaatca
      841 acttctggag gcagcccggc ttgaatccaa tcgggttcag tttcatcctt cactacagcc
      901 gctggttcct tttgttcgcg aactgacttc tatgtttgaa tcggcggctc agcgtaaagg
      961 cattcatctt tcgctggaga cctacgacga gaaaatacaa ttgtactatg accttcatgc
     1021 gatggaacgc gttttgacaa atcttatttc taatgcgatc aagtttaccg agcaagggtt
     1081 tataaaaatt acgatcaaac atagtgaaca tacgcatcgc ccgtcggttg tcatttttgt
     1141 cgaagacagc ggacgcggca ttccgcagga acatctcgat catatctttc agcgttttta
     1201 tcaagcggat ggtgtcgaag ccgatcaact ggggagcgga ataggtcttt cgctggtcca
     1261 cgattatgtg gagatgcacc gcggcttgat cactgtcgaa agcacggtcg gcaaaggcac
     1321 acgttttatg ataacgcttt ttaccggcga agaacattta tccggtacga tcacacctca
     1381 tgctgaaata aaaacggaaa ccgaatttat ttacgcccgc tccatcgctg cactttcatc
     1441 cgaacataac attgcaccgg aaatatcagc gccggatccg atggaacgtc aaaccgccga
     1501 taagcctaag gtattgatcg ccgatgataa cgccgatatt cgtttgtact tatataatct
     1561 tttgcgggac gaataccgca tgatcatggc gcacgacggc gccgcggcgt tggatttgct
     1621 tcaacgggaa tttccggata tggtgctcag cgatgtgatg atgcccaata tggacggaat
     1681 ggaattgtgc cgtcgtatca aatccgatga acgcacccgc cacattccgg tcatactact
     1741 caccgcacgg gccgacgaat ccagcaaagt ggaaagcctc aatctgggtg cggacgattt
     1801 tgtcgccaaa ccttttagcg ctaatgaatt gaaagcacgc atgcgcaatt tactcggcat
     1861 ccatgccatg aaactgcagt tgatcgaaca agaaaaaatg gcttctttag gacatcttgc
     1921 ccgcggtgtc gcacatgaaa tcaataatcc gctctccttt gtaacaggta atctttccgt
     1981 tttggaatca cggctagcca aactacatcc ggatgagaat attaaaccca ttgtcaccga
     2041 tatcatgagc ggaaccaaac gcattgccgc gatcgtgcaa cacttgcgtg aattcacacg
     2101 tctggatgaa gccgaacgta aatacgttga tctgtccgat atggtggata acattctcac
     2161 gctcttagcc ggcgaaatgc aatccgatat tcgcatcgaa aaaaatatcg cacaggtgcc
     2221 catgattgat tgttttccgc gcgatctcaa tcaagcgata gcacaaatcc ttatgaacgc
     2281 gacatattat gtacgtgaac gcaatcgcat tgaaaaccga tctcttggtc atattaccat
     2341 tcatctgaac aaagccgatg agaaatatgt cgaacttatc atagaaaacg acggcttccc
     2401 gattgcaccc gaaattcgcg aacatatatt tgaacccttc tttacgacca aacccatcgg
     2461 caaaggtacc ggactggggt taagcgctgt ttacggcatc attcgccgtc accacggaca
     2521 gatacgctgc gtgtcggaac cgttccagcc ggtgcgcttc attataatgt taccgtttgc
     2581 aataccgtca taataccggt ttttgatata tgtaattttc gcaaaattga tttttatcat
     2641 cccgactcct catcccgatc atagacaaat ctcccataac atgatagttt aaggcagagc
     2701 tgcaaaacag ctctcctttt agtttaaata aataactaaa tggttatgta actaattagt
     2761 ttttatgtac ttccactttg gagagatcgt atgaaatcta ttttacactc catcacccgt
     2821 atcctgacac tcttttatat actggctttt ataaatcagg cttgggctac cgacggatat
     2881 ttttcacatg gctatggtac caaacaaaaa agtatgggcg gtgtatccac cgcgttgacc
     2941 tttaatgcgt tttccgccat cacgaacccg gcgaacgccg tttatattca aaacaactgg
     3001 ggactgggta ttgctttctt taatcccaac cggcaatacg ccgtaagcgg ttcaccttcc
     3061 ggttttccca atacgtttcc gctcacaccg ggaacggtca aaagtaaaag tacgggattt
     3121 tttattccat ccctgagtta caatcgcctg atcaatgaaa aatccgcttt caattttgct
     3181 atttttggca atggcggaat gaataccgat tataaaacca atacgtttaa tacgaccaaa
     3241 cccacgggtg ttaatctatc acaacttttt gtcaatacga gttatgcata taaagtgacc
     3301 gacaaccatg cttttggcgt taccgcgatt ttcggatacc aatggttcga agccaaaggg
     3361 ctcacagcct tcagcggatt ttcaagatac aacaccaaac tcacggacaa taaatcggat
     3421 aaagcctacg gatacggcgc tcgtctgggt tatagcggac agatcacgcc gcagttgcgc
     3481 atcggcgcat cgtaccaaac gcgtatatgg atgaccgaat tcaacgacta caaaggcctc
     3541 tttgcggaaa agggtgattt tgatgtgccg gccacatgga atgccggatt agcatgggat
     3601 attaattccg catggacgct cgcggcagac cttactcaaa tctattatag ctccgtcaaa
     3661 tccgtcggca atccgatgaa accggctgat tttcaaaacg acattttact cggcgacaaa
     3721 aaaggcgccg gatttggctg gaacgatatg acggtttaca aaatcggttg cgaatatcgt
     3781 cacggcaacg attggtcggc ccgcgtgggt tacaactacg gcaaacaacc tatccccaca
     3841 agcgaagtga tgtttaacat cctcgcaccc ggcgtgatcg aacatcatct tacgtttggc
     3901 gccagcaaaa agatcggatc gaaatacgaa ttcagtttgg ctgtcatgcg cggtttttcc
     3961 aatgaagtca aaggcgccaa tccgatggaa gctccgggac aacaagcgat tgcactcaag
     4021 atgaatcaat gggaatttga aatcggttta ggtttctaat atttaacagg cgattacatc
     4081 atgttatcac gcagacgttt tttaaaaatc ggagccggat ttacagccgg tgcaaccatc
     4141 gcggccggcg ccgctcatac gttggctgat cgtttagccg cgttggaaaa accctccgga
     4201 caggtagaag tgataccgac gtattgtgat atttgttttt ggaaatgcgg tgttttggcg
     4261 catgtcaaag acggtcagct ttggaagttg gaaggtaatc cggaagatcc gcttagccgc
     4321 gggcgtctgt gcccgcgcgg cacaggcggg gtcggcgcac actacgaccc ggatcggctg
     4381 cgtacgcccc tgatccgccg cggtgaaaaa ggcgccgaag aatggacctc cgtcacatgg
     4441 gatgaagcgc tggacttcat cgccgaaaaa atgaaaaaaa ttaaagccga atacggcccg
     4501 gaatccgtgg cgctttttag ccatggtatc ggcggtaatt tttttaagca tacgatcaaa
     4561 gcctacggct cacccaatat cgcggcgcct tcgtttgccc aatgccgcgg tccgcgggat
     4621 gtcggatttc gcctcacctt cggcgaagat gtgagttcac ctgaacgcac tgacatccgc
     4681 aacgccaagt gcctcgtttt gatcggttcg catctcggcg aaaatatgca caacacgcag
     4741 gttcaggaat ttgccgaagc cgtagaccat cacgcgacga tcatcgtcgt ggatccgcgc
     4801 tattccgtcg ccgccagcaa agcgcagcat tatctcccga tcaaaccggg taccgatctc
     4861 gcactgatct tggcgtggat gcatgtcatc gtcaaagaaa atctttatga taaagcgtac
     4921 gtcgaacagt atggttttgg tttcgacgca tttgttgcgg cgttggaaac ttacacgccg
     4981 gagtgggcgt atcctgaaac cggcattgat ccggaaacga ttcgcaccac cgcccgcgta
     5041 atggcacaac acaaacccgc gactctcgtg catccgggac gtcacgtcac atggtatggg
     5101 aatgatgccc aacgcagccg cggcattgct ctgcttaatg cgctgctcgg cagctgggga
     5161 agcaaaggcg gtttttatac gccggtcagt atggatgtgc cggctttccc ttacccctct
     5221 tatcccaaat cggataaagg taaagtggat aaccccggtc ataaatatcc ttttgcgcac
     5281 gaaacgatca ccaccggcat acgcgaagcg acactcaccg gtaagcctta tccgatcaaa
     5341 ggttggtttg tgtacgcaac caatctgctg catgctttgc ctaacgagga agaaacaatc
     5401 aaggccattc agaatcttga tctgatggtc gtgattgatg ttattcccag cgaaatcgcc
     5461 ggctgggccg atgtgatatt accggagtcg gtgtatctcg agcggcacga cgatcttaat
     5521 gtcgaatggt tccgcgaccc gtttgtcgcc ctgcgccaac ccgtcgtgtc tgccccgcat
     5581 gaccaaaagc ccaactggtg gatggccaaa cgccttgccg aaaaactcgg gctcggtcat
     5641 tattatccgt ggacccatat cgaatcgtat tttgaaacgc ggctccgtca ggcaggattg
     5701 agttatgata cgctgaaaaa aaacggaatc ctcctcggcg aaaaaaaacc ggtttatttc
     5761 gacgaaggcg taccggccga gtttcccacg ccatccggaa aaatagaatt ttattcatcc
     5821 caactcgctg aagccggttt tgaaccggtg cccaaatatg tagccccgcc tcagccgcca
     5881 ccgggttcat ttcgactttt gtttggtcgt gcgccggtgc actcgtttag ccgcacgcat
     5941 accaaccccg tactgatgga tatgatgaat gaaaacgaac tttgggtcaa tgcggacatg
     6001 gcgttacgct acggtttaaa atccggcgat tatgttcgcc tcaaaaatca ggacggcgtg
     6061 gtcagcacta aaatcaaaat caaagcgacg gaacgcattc gcaccgactg tgtgtatatg
     6121 gtgcacggat ttgggcataa cgccaaagcg ctcaaacgcg ctttcggacg cggcgccagc
     6181 gatgcccagc ttatcacacg ctacgatacc gatcccttga tgggcggcac cggtatgaat
     6241 aataattttg tcactctgga aatggaggct taaccatggc gcgtctcgca atggtcatag
     6301 atacccgaaa atgcgtcgga tgcatggatt gtgtcgtggc ctgcaaaact gaaaatcagg
     6361 ttcccgaagg atacaatcgc gactggatca ccaccgaagc caaaggaaaa tttcccgacg
     6421 tgcgtctcga aattcgatcc gaacgttgta atcactgttc caacccgcca tgtgtgacct
     6481 gctgcccgac cggcgccagt catgtgcatg actatggcgg cgtcgtgctg gtcgatcatg
     6541 atgtatgcat cggctgcaag gcatgcgtcg catcatgtcc ttacgatgcg cgttttgttc
     6601 atcccgaagg ttatgtggat aaatgtacat tctgcattca tcgtgtggaa aagggattag
     6661 aaccggcttg cgtatctgtt tgcccgacgc attgtatgta ctttggcgat ctggatgacc
     6721 ctaatagtaa agtatctcag cttctgcaga aacgtgctta ccatacgaat ttaccggaag
     6781 ccggtacgca accgaatatt ttttatctca cgtgaggatg ctatgaacga attgacacat
     6841 acgcgcatga atccgctgat tgatcccgca ctgcacattt ggggttggga aatccccgta
     6901 tatctttttc ttggcggatt ggtagcaggg tttatgattt tgtccggtta ttttacgctc
     6961 aaaggccatc ataaacacga tcacttttct tctttttatt taccgcatgt gagtttggct
     7021 ttactgagcc tcggtatgtt ttccttattt ctagaccttg agcataagct ttatgtatgg
     7081 cgtctttaca cgacatttga aatcacttcg ccgatgtcct ggggatcttg gattttactg
     7141 ttagtctatc cttcgttact tctcaatacg ttggtgcgca ttccggaggc ttttagtaac
     7201 cgcatacatc tgtttgataa attttcggaa cgcatccatg cgcatcctta tctcatcaaa
     7261 aatatcggcg tgctcaatat gatcgtgggt gcgatattgg gaatatacac gggtgttctc
     7321 ttgagcggac tggccgcacg accgttgtgg aatagctcta tgctatggat actttttctc
     7381 acgtccggaa tatccgccgc ggccgccttc gttcaccttg tcacgacaga ctctgtcgag
     7441 cgagaaattt cagcgaaagc ggataatgca tttttgattt ttgaattgtt tgtaatcacg
     7501 ctgttcatcg taggtatgtt gagttccgca caagcgcaac aaaccgcagc aagactgata
     7561 ttaaacggtc cttacgcttc ggtattctgg gtatttgtgg tcggttgcgg catcatcatt
     7621 cctttgatca tccaactgct ggcggtcaat cataaaatac aacatacgcc gatagcgccg
     7681 atcatggtga tgctgggcgg gctcatttta cgatttgtta tcgtatatgc cgggcaggct
     7741 tcacactgga ctattgtaca ctaatattcg gagagtaatc tatggcatct tcagaagaac
     7801 attttaatga acatcgcgcc gtgaagccac cgcagccgta ctggaatcca tatctgtccg
     7861 gcatcggcct cgggttggtt ttgctggcgt cgtatctgat catggggcgt ggacttggcg
     7921 catcgggtgc gtttactacg acgacggccg ttgctgtgaa tgccgtagcg ccggagtatg
     7981 cacaccaaaa tacgttttac agcgaatacc tcggtgacgg cacacgcagc ccgctcaaag
     8041 attggctggt ttttgaagtg ctcggcgttt ttgtcggcgg attggtttcc gggcttctag
     8101 ccgggcgcat caaaaaatcc gtcgaaaaag gcccgcgcat ttccgtccga tcacgcctct
     8161 tatttgcttt tgcaggcggc atgctgatgg gcatcggtgc aaaacttgcc cggggttgca
     8221 cgagcggaca agccttgacc ggcggcgccg tacttaatct cggaagctgg gcatttatgc
     8281 tgatggtatt cgccggcgct tacggactgg cttatttttt gaggaggcaa tggacatgaa
     8341 cgcacctttt ttcaaatacg gattattcgg tgacgaagcg agcctgatcg tcgcttttat
     8401 catcggtatc gggtttggtt tttttcttga acgcgccgga tttggcagcg gtgtaaaact
     8461 cgccgcacag ttttattttt atgatatgag cgtactcaaa gtcatgttta gtgcaatcgt
     8521 aacggccatg ctgggcgtat attggttctc atggtttggc ttgcttgatc tcgatctgat
     8581 ttatatcaat cccacatttc ttctcccgca aatcgtcgga ggtcttctgc ttgggttcgg
     8641 atttgtgatc ggcggatatt gtccgggcac ttcggtcgtg gcgtgttcga caggacgcgt
     8701 ggacgcgatg gtttatgtgg ccggtatttt cggaggaatt tttacgttcg gatttatttt
     8761 tccttacctc gaatcgtttt atacagcgac gccgatgggc gcgatcactc tgccgcaatg
     8821 gctcaatctg ccgtacggca ccgtcgtatt tcttgtcgtt gtcatggcga tcggcgcatt
     8881 tgccgccgcc gagtggggcg agagaaaaat ggctccccga ggaggtgcat cgtgaaaaac
     8941 ctatggcgtg atctcacgtt aacgcagaag ttcggcgtcg tcgcgctgat gctcggctta
     9001 ttggcactga tcggcggtac accgcaacgc ggttcttttt atacgataga cagcaaagcc
     9061 atgctgatgg acgtcgccaa agagaccgat cacatcgccg tggaaacatt ggccgactgg
     9121 atcatccaag gccgttcgga ttacagactg ctggatatcc gcgacgccaa agcgttcgag
     9181 acgtaccaca ttccttcagc cgaatccgtc ccgctcgccg aactccatca ggccgaattg
     9241 cgccgtaatg aaaaaattat tttttactcc gatggcggta ttcatgcttc gcaggcgtgg
     9301 tttgtactca aagcccgcgg ctacaacgga gtatatatgc tgcggggcgg actggaagaa
     9361 tggcaggatc gtattttgta tccgcacaaa ccgtcaacaa ccgatacgga atccctgagg
     9421 cagtttgaaa agtccgcggc ggttgccaaa ttttttggcg gcgaaacaca aggcgaacaa
     9481 tctacagcgg ccgtcaaatc agcgacgaca ccgctgccca aactccctgc gggcgctaaa
     9541 gccgccgcac ctaaaaccgg aaaaaagaaa aaagaagggt gctgatcaaa tttatatgac
     9601 ttttcgcatg gctaaatccc ctacgggata ggatatttac aaaaacgtgc gggctgacga
     9661 tatgcccata gattgataca tatgattttg taatacaaag acgttcctca aagaacgtct
     9721 tttcttttgg atacggtgaa aaccgtttgc aacttctgtt tttgtttgtg aaatttaaca
     9781 cgcatgtatc ttcatatttt ttacctacgc tgaacggccg ccaggccgtc ttcaggtttc
     9841 ggagaatcat catgagacgc ctttcgaact ttattttgat tatggtcttg agcctaccgc
     9901 tatattctca agacacgctg acattaaaaa atatttctta ccaacccggt cagaaattat
     9961 ttgtcggact tgaaaacgaa gaccttggag cgccgttgcg tgtccaattg ctcaacgccg
    10021 acgggaaacc cgtaagtaac cggatcgtcc tatttgagat catcgaacgg ccttcaaaat
    10081 ctaaaggcga aaaactcggg caagctgaag cgataaccaa tgccgaaggt atggccgaaa
    10141 caagttttcg tctcggtgat catgcgggtg attatatcat cattgcccgc acgaccggca
    10201 tgaccggtga tacaccgcat atcatagttc gcgcccaacg cacgatgtgg tggatgtttt
    10261 tgattttcgg attgatcggc ggcttgggca tatttttgta cgggatgaat ttgggttccg
    10321 gcggtttaca aaaagtagcc ggcgatcgtc tacgtactat tctcggcgca ttgaccagta
    10381 accgtttttt gggattgctt gtcggtattg ccgtgaccgc catagtgcaa agcagttcgg
    10441 ccacatcggt gatggttgtc ggattggtca gttccgcttt gatgacatta caacaagcca
    10501 tcggcgtact catgggcgcc catatcggca ctacgatcac cgtacaattg atagccttca
    10561 acgtttccga ttattctttg ttgctggttg gcgccggatt tcttatgacg attgcaacga
    10621 aacgaaaatt ttacatctat atgggtgaaa tcatcctcgg attcggtttt atatttttcg
    10681 gaatggccgt gatgtcgcag gccattaatc cgttaaaatc catgccggct ttcatcgcgt
    10741 ggctgatcga atttggaaat tctccgattc tcggtattct cgcctccacc gtgtttacgg
    10801 ccattattca atccagcggc gcgacgatcg gtttgtgtgt cgtcatggcc ggagaagggc
    10861 tgctttcttt acaatccgcc atgccgcttg tatttggcgc caatatcggt acatgcgcaa
    10921 cggctttgct ttctaccatc ggtgcgacga ccgacggcaa acgtacggcc ttggcccata
    10981 cggcattctc cgtgatcggc gtatttttat tcacgccatt cctcaccccg ttcgaatcac
    11041 tcatccagag tattacctcc tggatgggta ccgaagccgt ggcgcgtcag attgccaacg
    11101 ggcacatgat attcaatatc atgacggcga tgatcctgat cggatttgta ccactcatga
    11161 ctcaattgat caccaaaatt attcccgaaa aagccgaagg ggatgcgttc aaaccgaaat
    11221 atcttaacaa cgcctatctg gaaactccgg acattgcttt gcagaatgct cagcgtgaag
    11281 aagtgcggtt ggtcactatc gtacgtgcca tgtatgacaa caccatgaat cttttttcct
    11341 cagaaaacga ggaaaaagtg gccaaagtgc gcgaacagtt gaaacaagtg aatttcctcg
    11401 agggtgaaat ccggcgttat ctgacgcgtt tatcgcaaaa gagtatcagt ttgacgcaga
    11461 gccggcgact tatgcggctt ctgctcacca ttgatgattt gcggcatatg gccgaacgcc
    11521 ttggtgacag catcgccaat ctggccgaac gttatgcgga taatcacgtg gcgttttctc
    11581 cggaagggct ggaagagcta aaacgtttct acggtatgat acatgaacgg cttgacgcgg
    11641 tactcacagc acttgaaaaa gaagacgccg tcatggccga acatatcatc aaaaacaaac
    11701 atatggtttc cgaagaggaa gtgcggttgc gtcagttgca catgacacgc cttcaaggcg
    11761 gtaacgaaca actatttgtc agcagtcaat atcactttga tttgcttacg gcattacaac
    11821 gagttcatat tttctgcgtc aagatggcgc atgaaatagt agaacagcat aatttataac
    11881 ggatattgtt tagatataaa aaacctccga ttgctcggag gttttttata ttaaggatcg
    11941 cataatcatc aaaacaccgc cggcgaatca gaactttgtc actacacgaa tgaaaattca
    12001 attacaagac cattgagaat tcgttacgcc attgcgttat agcgagaacg catccttctc
    12061 gcaagccgca aagaaaaatg gaaaaatatt ttacgtaaaa ctttccattc caagatccat
    12121 ggcttcttcg atcgcaaaag tcggcggcgg aagttggcgt atcatggttt cttgtagcat
    12181 cagcgctttg atttgattgg aaacgcgcga cgactgatga tcactgagcg cgtacagaaa
    12241 aagttctaac gcacggcttg cattcttttt gatcttcact acgtctttat cgttgatcgt
    12301 catgagttcc tggatttcgg cattgcccgc aaaattggct aatattcgat ccgggttgct
    12361 gaccatagct tgggcctttt tatatacgat cttggctact tcgaggtatt gccccaataa
    12421 ttgatcatct tccgtacgcc ccaaaagatc aacaccgact ttgagtgaat acatacgatc
    12481 tagaaattcg cgtatcaatt cgccgcgaat caacgacccg tgttgcggag cgatcatcac
    12541 cggcgccgga tcaagcttgc gtatctgatc aatggcgaat tgaatcgctt tattggccgg
    12601 catgtagagt tcgtgaaatg cgcgcattcc gtcccaatgg ctttcatcgg cccacaatcc
    12661 gtaattgtcc gtgccggtaa ggcctccgaa taaatcaccg gaaaaaagaa tgcgggattc
    12721 gatatcatac accatcacgg cgccgcgaaa atgacaatac ggcgtcggaa taaattgtaa
    12781 cgtatgcccg gtcggaagat tggtctgcca gttggtcaat ttatcggtaa gaatcaaacg
    12841 ttctttatta ataccaaagc tggaaacgag ccgccatgtg tcttccgatg taaggcaaat
    12901 ggctttaggg ttggccgact tgaggaaaaa cgtattggaa gctacatcgg gatcctgatg
    12961 attgaggctg tataagttga ccttggacac atcaccgatc acctcgccga ctttgccgga
    13021 aatcgtcgta tagtcaatcg tcggtccggg atcaaccaaa aaattgacct tattattata
    13081 cacacgcaaa aatgggttaa tctgaagaat acgcgattct tttctgtcgc cgacaaaata
    13141 aatatcttcg gcaatcgcta taggtttaga aaaatcctca cttttcatcg cgtttccttc
    13201 cttcgttgag tgcaccgata ccggtatttt gttttcgctt tcggttaatt cagtggggcg
    13261 tcgtgcgaga tagtatgaac cgctgctcca ctgtaacatc gtctccagcg cttccgagtc
    13321 ttttaattca ccgcatacga tcgtttcgca tacaccgcga ttaaactcga tcgagccgac
    13381 gccatgatcg ctttggatag tcaaaatccc gtgaatccct tctttttccg ctgacgcgat
    13441 gagttcttcg atttttccga gcacgagcga accgcttcgc aggatgccgc tggaccgctg
    13501 ggcttccaca aatcgaaaca gcgcctgaat tttcaaaaac agcaaattca gattgatcgg
    13561 ttttggtaaa aaatcaatgg cccccatttg gatggctttg gccgtcaatg acggatcttt
    13621 gtcgcccgac atcagcagaa acggtatgga cgccaggcgt ggctgtctac gaacagcctc
    13681 tataaaaccg aatccgtcta attccggcat cataatgtcc gatatgatca aatccggtga
    13741 ttgaccggcc aggatcgtaa gcgctgcttt gccgtttaac gctgtcacta ccgaataacc
    13801 ggattgcccc aaagccaaac ttaaagcgtc tacggaagtt ctttcatcgt ccacaatcaa
    13861 tatcgtcatt taaaaaaacc tttccgttaa tatgaaaatt aggactgctc agcggaacgc
    13921 aacggtgcga gtacgtcgtt caacgtttga agtatttttt cagcgcggta tggtttcatc
    13981 aaaaaagcct gtactttgaa tccgtttttt tgaatgaact tttcattttc cggaagaccg
    14041 ctcatggcga tgatggggac atcttcacga atactgcgca acatgcgaat aaacgcatgg
    14101 ccgtccatgt gtggcatgct tatatcggtt atcacggcgc gaatggaagc catttgggcg
    14161 gcgaataccg tcatcgcttt tccgccgtcc tccgcttcga ctatttggta tccattatgc
    14221 gtcaggatcg ttcccaatat tttgcgtatg gccggttcat catctacgat caagatcaat
    14281 tcaccacggc ccaataacga ttccggaata cgagactcaa ccgattccgt gtggaaaccg
    14341 gcgctggcgg ggatgtaaat tttaaactgt gtgcctttac cgacctcact atacacgctc
    14401 acaaagccgc catgtccttt gataatcgcc gcaaccgtgg atagccccaa acctgtaccg
    14461 tgttcgagat ccttggttgt aaaaaacgga tcaaagatat gatctatgat atccgcagga
    14521 atccccagtc ctgtatccgt aactgtaaac agaacgtacg ggccgggttt ggcctggtgt
    14581 tccttataga catacgcctc atcaaaaatt acattttcga aatggatcgt cagatcacct
    14641 cccgatggca tggcatctcg tgcattgacg cacaggttga gaaatacttg atgaagctgc
    14701 gtgctatcgg ccgtgatcgg atacagatct ttggctacgt tgcaaacaat tctaatgttc
    14761 ttgggaaacg tcgaaacaag aatacgctcg atttcttcga tcgttttttt ggcgtccact
    14821 acacgatgtg cgccttctgc gccgcgggaa aacataagta acttacgcat cagatcagca
    14881 gcccgttcga tattagactc cagcatgacg atcaaacgct gacttgtatc gtctggtaat
    14941 ttggatttga acatttgaac agccatcaag atcggcgata aaatattatt gagatcatgc
    15001 gcaataccgc tggccatacg accgatgctt tccatacgct gcgctctaaa gtactgcttt
    15061 tcaagccgtt tacgttcagt aacgtcagtc tcgatataca aaaatgattt gacggcgcta
    15121 tccgcatccc gcacagggct cttgcggcat tcgacgatga cattccttcc gtcccgcgcg
    15181 taatgctcga tctcaccgct ccacggcgca tcaccgctga ataatatgcg cgccgcagca
    15241 tccgtcgcgc gggtatgccc gagaacttcc gtcgcttgat ggccgatgac attggctttt
    15301 tcccagccat acaacgtttc gcattggctg ttccaatata ttatccgccc ttccggatcg
    15361 gtcataaaaa tcgcatcccg tgcattatca aggatcattg cttgctcttg gataatctct
    15421 ttggcttttt tatgaaatgt gatatcccgg cctattacca ccaatcccaa ccttgctccg
    15481 ttttcttgta acaaagggat cttcgtcgta tcaaaatgca ataaatcacc gccgggtttt
    15541 tcgataattt ctatggtttg gtatcgctga ccattacgcc acgcggcctc atccgttcgc
    15601 acacatcctt cgtacgccaa acgcaaagcg ggaaaagctt cagccaactg ttgatccgtt
    15661 ttaccgtacc acggatacgt cgccaattca aaaagtttga gcgcggcagg attggcgatc
    15721 aaccatcgcc cgtccccgtc tttaaaaaag acagcgtccg gtatcgcctc aactaatgtt
    15781 tcaaattccc gcaacgtttc gcttaatttg acttcggcct gttcgcgttc cgatgataga
    15841 cgatgcttga gcagcgccaa ttcgatttcc cgcgacacgc tgtcgaggaa attcagttct
    15901 tcttcttgcc attggcgggg gtggatcatg tcattgacaa ccaaaagaaa aaatccatcc
    15961 tcgcgaaatg agaacggata ccaaagcaaa ctctgtactt tcataacccg atgcaatatt
    16021 tcggcagacc cttcttctac aaaacaagcg tgtggcgcat caacggtact cgtgatgcag
    16081 gttcgtgtac tgtgtagtcg gcttatactg gatgcgaata cggaggtggg atacacctcc
    16141 tgaggtgggg caaaatcagt aaaatccggc cgaatccatt tggcaataga aacggcttcg
    16201 ccgcccgtaa tacgaatatc aaataaaagt gcgcgctcta tatcaatggt ttcaccgcaa
    16261 atggtcgtca tttcccgcaa caacgtttcg gctgtaccgt ccgaattcat aatagaagcg
    16321 gccatacgat tgagcgcacg ggcgtagcgc agttgctcgc ggatatgatt ttcatattta
    16381 cggcgacttc ggttatcacg gccgatacat acgatgaccg gcgttcccga cgacacggct
    16441 ttacttgtac gcacatcaaa gggaattact tcaccgttta ggtgaagaaa atcaatttcg
    16501 cgcgacacgg cgccgatttc atgactggcg ccgccggtga ggtacgatat cgcgtcatag
    16561 tccgcattac cgggcaaaat cgtgctgatc atttttccgg acaaggcgcc ttgcacataa
    16621 ccgagatttt gttctacagc cggattcgca tagatgatat agccgttgtt ttcaaataac
    16681 aaaaccatgt cctgggatgt ctcggcaagc gtgcggtatt tttgctcgct gtggagtaac
    16741 gcttcctcgg cgttatgccg ttcggtgaca tctttaaaat taactacgat cgcctgtact
    16801 tcgggcgctt gaagtaaatt cgtaaacgta ctttcgatcc atttccacga cccgttttta
    16861 tgcttgaagc gatacttcgc cctcgcgtca atatccggat tttcgatcac ggatgcgata
    16921 agctgttcga cacgctcgac atcatccgga tgggtcaatg cggcgggctc cagatcggac
    16981 aactccgaca tttcgtatcc gaacatgcgc gccgaagaag gactgatgta ttgaaatttt
    17041 ccttcggcat tgatcagcac cacgccgtcc ggtgcatgtt cgataagcgc ttgaaaatgt
    17101 ttctcgacgc gacgacgctc cgaaatatcc cttacaatac cctgaatgcg tccgtcatca
    17161 agcattttgg cgctaatttc aacttcgaca tgtgtcccgt ctttacggac taaacgccga
    17221 tgcgataaaa tcgattggcc cttttctaat tgcctgattt tgagcggttg gcggattaat
    17281 tcttccgcat ccaacaaacc gtaaaaaggt tgcttcaaca attcgtttac ggtatacccc
    17341 atcaagcggc aaccggccgg attgacatct tcgatttgtc cgtcacgggt agccacaaaa
    17401 ataccgtccg cggcctgatc aatcaacgta cgataccgct tttcactggc acgcagcgaa
    17461 tcttccgccg attgacgcac catctgcgat aacaaacgat ctatcgtcgc cgacatatgg
    17521 ctcgctaaag tcgtgagata ttgaagggcg gattcatccg gcacacgcac cccttcatca
    17581 tcataggttc ccataccgat ggaagctaag cgcccttcgg aaagatgaac cggtatattt
    17641 acgatcgtac gcactccggt agactgtacg atttttttat tggttcgtgc atcctccgcc
    17701 gcgtcgggaa taaccactgc acccgttccc cgagccactg cactcagcat cgcatcattt
    17761 tttatatcaa ggcgtttagg tgcaaacggt cttggcatgc cggcctcggc caatacttct
    17821 gcttcggagc gatcctccga aaaaacgtaa acccataatc tctgatatcc caccagacgt
    17881 tgcacttcgg ttaatgccgc ctgggccgct tcaccgaaac ccgcgactcg ctccagctga
    17941 cgcgctaaac gcaaaagtga ttcggcttgt gattcttttt gcctgagcgc cagctcggcc
    18001 agtttacgtt cggtaatatc gcgattgctt ccccgaacac ctcgaaaaac accggcagcg
    18061 tcggaaatag gctgacagac atggccaatc catttgatga cgccaccttt acagacaata
    18121 cgaaaatcca gattacccgg taaacgccgc tgctgaatat cttgctggtg agctaaaaaa
    18181 gccgcccggt cttcggcata tactatgcgt tgcaaaaaat cacgatccgt cgtaaaagaa
    18241 accggcggat atcccgtgat acgctcacac gaaggcgaac tgtaaatgta ggaaccgtcc
    18301 gtatccagcc aaaactccca atcaaatgta ttatccgcta tgatctgata tttgatttcg
    18361 ttttgcttta attgatcttg cgcttgccgc gtttcgtttt cagaacgaat ttttccgctg
    18421 atatctttcc aaatgccgat aaaatgcgtt atctctccga tttcgttacg aaccggagag
    18481 atcgtgtgct caacgggtat gatgctgccg tcttttcggc gattatattg tatatttcgg
    18541 aatacgctac ccgataatat tttttcccac atcccgctat aaaaagcttg ttcatggtag
    18601 cctgatttta atatccgcgg attctttcca agcacttcgt cacgcccgta tccggaattt
    18661 ttttcaaacc cgctgttcac atagagaata tggccatctt tatcggttat gatcacagcg
    18721 tcatccgttg actccaccgc tcttaagaat aaatccgatg caatggaatt atcatattgc
    18781 ttcggccagt gtgatgtcgg cgatagaaac gatagaacat tttttttagt aaaaggaaga
    18841 gggataactt catccgactt cagcacaccc tctttcacga gccgttttcc ttgggccgca
    18901 ggcaaagcaa acccaacacg caaattcgcc gcgtgcatca ttttcttttt taagacaagc
    18961 gctgaagcta ttctacggta ggttgtagtt aggatggccg atgaagatcg gatttgagtg
    19021 actttacgca gtgcattccg atcggttata gaaacaacct cccattgcgt gagagccgtt
    19081 aaagcggacg cctgcgccgt gctacctaaa aataacacta tctgcgtatg tgatttaccc
    19141 gctttattca caacgccata ctctcattat tttttaaatt accacgagaa ccctactaaa
    19201 atttagttag aataatacaa tacgataata attctcatga ttttcatcat gattttaaaa
    19261 aataattgta aacaaattta aaaaaaggat gggagttatg agagaataac
//
LOCUS       DAOQNQ010000035        18648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1041_length_18648_cov_5.756952, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000035 DAOQNQ010000000
VERSION     DAOQNQ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18648
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1041_length_18648_cov_5.756952"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="PK754_03995"
     CDS             <1..811
                     /locus_tag="PK754_03995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB08696.1"
                     /translation="GANASKVMDNDDLIALVIVLRNITKEKEIDRMKTELVNVVAHEL
                     RSPLTSIAGFSEIIKDPHLSEAIRKEYIDIIHYESGRLAEMISKFLDISRIESGKTVI
                     NKVPCDLTTVVDNTITANTPMAQKRSMHVSSTLPKWVPLVHADPDLIGQVVLNFFSNA
                     VKYSPEGSEIKMRVAVADDTVIVEVSDNGYGISKENMKHLFQKFFRAKDEKHVKDVEG
                     TGLGLAFVKEIIHQHNGTIHVESEVGKGSSFSFALPIDFTLPENAALKPAS"
     gene            complement(820..2616)
                     /locus_tag="PK754_04000"
     CDS             complement(820..2616)
                     /locus_tag="PK754_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB08697.1"
                     /translation="MQTQDDDIMGKAYDARLMRRLLSLVRPYRWMAVVSVILLIAAQG
                     FSAYRPKLVQEAVDAAILAGDGLRLRNFAILFVVLLIGEFLLQYCVIYVTQLMGQRII
                     FDLRMKLFTHLEHLHLQFFDRNPVGRLITRVTSDIESLNDMFTSGLVYLFGDIFLLAG
                     IIVMMFVLDVHLTLVTLAILPLIFYISVVFKSKVRVAFREVRLKIAALNSYLQENITG
                     VTTVQIFNREKKNFEKFDALNRTLTDSHLDSVFHYAWFYPAINVASSFAIGMLIWYGS
                     GEVRSSSITLGTLIAFIQYGMLFFRPIQDLSDKYNILQTAMASSERVFKLIDTPTEIP
                     NPAQPITPKVMSGRIEFRNVYFAYNPQDIRSDEDYILKDISFVTQPGQSVALVGATGS
                     GKSTIINLASRFYEAQRGDILLDQTDIRRMDQYALRRTMAVVLQDVFLFSGTILDNIR
                     LGRHDISEAQVEEAARRVGADDFIRRLPLQYLEPVQERGSTLSVGQRQLIAMARALVF
                     DPRILILDEATSSIDTETERLIQKAIAQLMQGRTSVIIAHRLSTIRHVDQILVLHKGR
                     IRERGTHEELLAMGGLYYRLYQLQYKDQEFIA"
     gene            complement(2623..3903)
                     /locus_tag="PK754_04005"
     CDS             complement(2623..3903)
                     /locus_tag="PK754_04005"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNB08698.1"
                     /translation="MSHTLSNRWTLHLRQSTHTPFVVLRLRFPVGIAHEPADQPGLCK
                     LLALTLKSGTSKQTALSIADALEYLAASLSVSVTHDAFTLHLTVSREQFEPALLIVHE
                     ILTDAVFPEHELQRARGQMKADCKKKLDMPGVLLGELFQSTLFGRHPYGRSTDGMESG
                     VDDLSRDGALRFYNEKLTACGAIVTVVGDVDENKMRTFVENNWASHFKNSIDINAIHM
                     DSPQPLGQRIFCLTDRPIAQAHIALGHTGPARGEPGHVPQMVLNYILGGAGLSSRLTH
                     HIRTQQGLAYSVYSSLTKRKFGGMFSVVLQTSAAHAPRAVTAIVKELEAIKADGVSDQ
                     ELEDAKLYFSGHHPFTMETIERMALLIEQGAFFDLPPDYVERELAELQSLEQDDLRKA
                     ADMIDPKHWVLAIIGNEALIRPPIQKLLHHYERQ"
     gene            complement(3945..4562)
                     /locus_tag="PK754_04010"
     CDS             complement(3945..4562)
                     /locus_tag="PK754_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HNB08699.1"
                     /translation="MQRKVRADDRPKESTQTLDQTSATRTQDPKPEPTQLTMTGIHDP
                     KWDKILNYMIGGLGLVVLILVISLVIRINSSGTSEAETQTNEVPAETSPSTTFDGTQS
                     RPQKIRIEVLNGSGVPKLAAKAADYLRSKGFDVVSTGNAPNSNYKKTIVQDRAGNLAG
                     AQQVAVALGVGESGIIQQKNPQLMLEVTVIVGQDYKSLKFTNPRD"
     gene            complement(4614..5123)
                     /locus_tag="PK754_04015"
     CDS             complement(4614..5123)
                     /locus_tag="PK754_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08700.1"
                     /translation="MKKQTIFLLIFGLCSVSLSAQLKTQTGREPIDVHGGITSAVPGS
                     LLSFIGSDRVTMTQSYAMTYYSGGGASGTLGMYSNSLNIRLSDPLVLRLNTGLMHQSF
                     GQNKNSLMQKNREILHGAELVYRPSRHFQMQVGYSNNPYYTGNSLFNPGPQRSGSGGL
                     FDDYGFMRP"
     gene            complement(5199..5942)
                     /locus_tag="PK754_04020"
     CDS             complement(5199..5942)
                     /locus_tag="PK754_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08701.1"
                     /translation="MVKFVGSLIFLFSVVGCASRHVLIEPESPIVFSKRARGGAASWT
                     GEELNAPSLTIYANGVVIRPAYNEGKRRWMLGHMKPQVFRERIKHIQNIRDSVKAMHR
                     KTADTSAAIPVIEWITGSDTLAVRGLGFSVQGGWLDSMHYYNQEMDTWEYEALRPYVA
                     SRIRLYVKKSGSARGAQWPVWPVRAVLPSSVYTRDVSYYEPNVTENSVVIEGVIAREV
                     QSAIGQTGVYEKFIADGMVYGIGYVIEVP"
     gene            complement(5935..6636)
                     /locus_tag="PK754_04025"
     CDS             complement(5935..6636)
                     /locus_tag="PK754_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNB08702.1"
                     /translation="MVKPDIHLRAIIFDLDNTLVDFMRLKERAIASAAEAMIDAGLEV
                     KKEDIISKIWTIYNSQGYEYQKVFDIFLENALGRIDHKILANGIVAYRQTREASLVTY
                     PHVLSTLNELVKRRFKLAVVSDAPTREAWLRLCYLRLHHTFDAVVTYEDTFQRKPNPE
                     PFRKALTQMDIPPEHALMVGDWAERDMAGAKQIGMKTAFARYGDTFDTRESGADYELT
                     DFRQLLDIAVEPFHG"
     gene            complement(6648..7889)
                     /gene="rodA"
                     /locus_tag="PK754_04030"
     CDS             complement(6648..7889)
                     /gene="rodA"
                     /locus_tag="PK754_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNB08703.1"
                     /translation="MVQRIKKFLQDLDYLILAPALMLCGLGLACIYSATFTSDQLNFT
                     KQLTFFFIGVVLMLMTVLIPLRLIDGASFTVFAVTIALLILVFFAGSVRYGARRWFTL
                     GPFGFQPSELAKIAAILFIARILALRQVNSSQVMNIIIVFSVAAIPMLLVMKQPDLGT
                     ALCVGSVALPILYWNGISLFSIFVMFSPIATVLVHIISGYSFNAFITMLFIILTILYF
                     SKRKTLIILGVFAVNVIVGLSSEPIWESLKDYQKQRILNFIEPERDMQGSGYQVTQSK
                     IAIGSGGLVGKGYLHGTQTQLNFLPEQHTDFIFSVIGEEFGFLGSLFLLALFGLLIFR
                     IVIIADAVDDKFASLALIGIASLFAFQLLVNIGMTTGLMPVTGIPLPLLSYGGSALWA
                     NMFLIGFVLNVAMRKKNYEIH"
     gene            complement(7873..9747)
                     /gene="mrdA"
                     /locus_tag="PK754_04035"
     CDS             complement(7873..9747)
                     /gene="mrdA"
                     /locus_tag="PK754_04035"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNB08704.1"
                     /translation="MNHHRVVTMYVSLAAILLMLLGRLFYLQIMAYDNFHARAEDNST
                     RTIDQIPSRGLIYDRYGRRIVENRPTFALTITLSEFDTLSTGEMQYVRSLTGMTDEDI
                     LKKLRGAWPYAPVVIKPEVDFVTIAHLEEERRFHRGIGYKVDINRIYADDITMPHLIG
                     YMSQITDNTIGYYKTAEFQDTDYKIGERVGVSGLEKFYEVELRGEKGYHRLETNAKGQ
                     VLRDLGISKHPRDGYNLYLSIDNDYQKFVESALGTRRGSIMVADTRTGEILAATSKPD
                     YDFRWFVDGITIDQWRELNENPDKPLFNRMVQSGYAPGSTFKMITALAGLEEGLVTPE
                     TYVECTGVYQLGGNKFRCWNLKGHGHMNVSSSLVRSCDVYFYELAWKLGINRLAKYAR
                     MFGLGEPTGTDIPNERRGLIPTTEYYNSRYGFNNWKGGAVVNLGIGQGEILVTPVQLL
                     QYAMIFANAGTYQTPHLVRYAEDSEGIRRVTFPVKHVAVKQEHMDMMRQAMFGVVNYF
                     EGTGKKAGLKAAQVAGKTGTAENVHGKVGSGTEGYHGWFVGFAPYENPEIAIVVLMEN
                     GGEGSDVAAPMSALVMNYYFNFVRNKKSFDPKEKDRKETPKNTTAPQAAVVEVHGTTN
                     "
     gene            complement(9754..10278)
                     /gene="mreD"
                     /locus_tag="PK754_04040"
     CDS             complement(9754..10278)
                     /gene="mreD"
                     /locus_tag="PK754_04040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNB08705.1"
                     /translation="MRRKRNILTFSLLGAAAFFYLMQVSFVSVFLAVGDNVPDLLLIL
                     TVFVSMRLGQIPGMLFGFTVGLWQDAMIDFYGLNALCKTIAAFATYFFAAERVMLIEK
                     YYFPIMIFFLSVIHAVFFYAIQSLDGVVDFWSLMFHYGLWNSLYSAVTAFVIYLSIPQ
                     RTLDYVRFNTIYDF"
     gene            complement(10282..11148)
                     /gene="mreC"
                     /locus_tag="PK754_04045"
     CDS             complement(10282..11148)
                     /gene="mreC"
                     /locus_tag="PK754_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNB08706.1"
                     /translation="MLWKLRNFYAGLKEILYVYRHSFVFLVLVIASILLLQNNPNQSM
                     IFLRKRVIYLAAMLGEKISSGPRWRDSDAVIKELQQRNIELASRNIVLEDAYMENVRL
                     RQLVEFRSRFPLSVRPARIIARESRPAISNIVLDKGSADGIRLHQNVINEYGLVGQIA
                     EVFEEHSVCQMLLDKNFRVASKIQRTRANGVVFWPGNPDEVAFYGVLKNLDVRIGDVI
                     LTSEYSEYFLPNFRVGVVTEVNNEIEGLFKDIRVQTAVDFDRLEEVFVVTDTSRTRSV
                     AVGFENYFIVKR"
     gene            complement(11188..12213)
                     /locus_tag="PK754_04050"
     CDS             complement(11188..12213)
                     /locus_tag="PK754_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNB08707.1"
                     /translation="MSLFDIFNNEIAIDLGTANTLVYVKDKGVVINEPSIVAINKRSN
                     KMMAIGKEAKEMEGRTHDELLTVRPLKDGVIADFEMTELMLQEFIRKVSPARFMGKRV
                     VVGVPSGITMVEQRAVRDSAEHAGAKEVYLVAEPMAAAIGVGIDIEASKGSMVIDIGG
                     GTAEIAVIALSGIVCDRSIRSAGDELTKSIITYFRVQHNLLLGEQMAEDIKCNIGSAV
                     PLAQELKMEIKGRDIVSGIPKTMKTDSEEVRRALEGTIREIVEGTKSTLEKTPPELAS
                     DLIDRGIVLTGGGALIKGLDERMRKETGLPVIVAEDPLTAVVRGVGRVLENLTKYQAV
                     ILKNRKY"
     gene            complement(12247..13800)
                     /gene="purH"
                     /locus_tag="PK754_04055"
     CDS             complement(12247..13800)
                     /gene="purH"
                     /locus_tag="PK754_04055"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNB08708.1"
                     /translation="MIRRALISVSDKTGIQSLAQKLHQAGVEIISTGGTASALSQNGI
                     PVVKISDLTQFPEMMDGRVKTLHPKIHGGLLALRDNATHMEEANRHGIGMIDLLAVNL
                     YPFEATISKPDVPLHEAIENIDIGGPSMIRSASKNYRSVIVLVDPSDYEPVMKEIMTS
                     GDVAQDTRYRLMIKAFEHTARYDALISSYFASKVTASSETLPEKINLTFKQIQPLRYG
                     ENPHQKASFYAANFHSKKYYDQLHGKELSYNNILDLDACVRVVSDFEKPSCVIVKHTN
                     PCGAAMHDDLYQAYQNARATDPVSAFGGVIGFNRRLNAATAEALSEVFVEAIIAPEFD
                     SGAIEILSKKKNIRLIRYDFEAARAQGSALPEIRKALDGYLIQDADMRRAGQTEWKVV
                     TRRAPNAEEMHALLFGWTIVKHVKSNAIVYAAADRTLAIGAGQMSRVDASELAVSKSG
                     KSGLSLKNSAIASDAFFPFRDGVDAAAAAGATAVIQPGGSVKDAEVIAAADEHNMAMV
                     FTSIRHFKH"
     gene            complement(14008..14388)
                     /locus_tag="PK754_04060"
     CDS             complement(14008..14388)
                     /locus_tag="PK754_04060"
                     /inference="COORDINATES: protein motif:HMM:NF022591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HNB08709.1"
                     /translation="MKYVSALVVLIALAFVQTGYSQMKVDGMKFGTAVEKSEVVGEAT
                     SFPANGSNVYCWIKVVGGEGQTVTMKWYHGETLKNEVPLEIKSWSMRTYAFYEVHRAG
                     TWKVEIVDSTGKVLQSGEFTATAQ"
     gene            complement(14546..15013)
                     /locus_tag="PK754_04065"
     CDS             complement(14546..15013)
                     /locus_tag="PK754_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08710.1"
                     /translation="MRLSKIWLYGIAMLAVVMQFGCGSNKAVMNSKITHVEKKETLGK
                     GEYYQFEITLEDKMEAIEVDGEWRVEDGGDRKARVMVMDEENFLKFKEEEPYKTQYDS
                     GEKIQHTFKIRLLNQFRAQKTLYYLIVDNPSEEDKKEVSFRLDLNYEWGAGSR"
     gene            complement(15090..16388)
                     /locus_tag="PK754_04070"
     CDS             complement(15090..16388)
                     /locus_tag="PK754_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB08711.1"
                     /translation="MRKIGLVLKREYISRVTKRSFLITTLVVPFLMLLLPVTIFLIAR
                     NNDAERIAVRDESGLYIQKLQDSRLVQFFYESMPLDSLKRVYRDTKYNYDGVLYIPAA
                     STDPNDGIKYFSSRGLGFTTTLYINSELSREQEKARLSEAGMTKDKIDAIKQRVSFET
                     IIMTEEGESSDNKVVALTTAIIMGIIIYIILIIYGVMVMNGVVEEKANRIVEVIVSSV
                     RPFEMLTGKILGVAAVGLTQLVSWIAIGALLFQGAMLLLGPQMGQFSPDALAQGGIKA
                     RDAEAITVFMKNLDSINFTELFFCFVFYFLGGYLFYAALYAAAASGANDAGDVQSLSF
                     PVTIPVILSFFLLQVSVNDPHGPVAFWASMIPFSSPIVMLGRIPFGVPWWQLALSMSL
                     LIAGFLSAVWVAGRIYRIGILMYGKKITLPELAKWMFYKG"
     gene            complement(16381..17289)
                     /locus_tag="PK754_04075"
     CDS             complement(16381..17289)
                     /locus_tag="PK754_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB08712.1"
                     /translation="MTDVKLSVRDVVKRYQSHTAVDHISLDVQRQSIFGLLGPNGAGK
                     TSLIRMITQITMPDEGAIRFDGQALHESHTRRIGYMPEERGLYKQMKVGDHVMYLAQL
                     RGLSLWDAQKKVQMWFERLHIMDWWNKKVEELSKGMQQKVQFIATVVHEPELLILDEP
                     FSGLDPINADLIKDEIHRLKDEGATIIFSTHRMEQVEEICDRIALVHLGRNILSGEVK
                     AIKQQFKKHHYRIQFDGILPQSGDTAYSIVSQKTGEAVIATQPGVSPNQLLTHLLSRV
                     EMYAFQEVLPSLNEIFIEHVKGYNHA"
     gene            complement(17315..17926)
                     /locus_tag="PK754_04080"
     CDS             complement(17315..17926)
                     /locus_tag="PK754_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08713.1"
                     /translation="MENDELRELWKQGQSLYPQTGMNEKDIRKLLEMRIRTAQLRIRK
                     KLLFELSGMLAILFMIGAVLFVQSHLPLISLAITVASFLFIIPNGFLFILLIRRVSSL
                     DYGIDLRARHTAYLRLHQKTIRIYLRSAYGFSMVLALIVLFIPMEAVNENYMMYFKLG
                     FMGFITVVTFFYRPYVRWMFDKDAQQMTAQLQALQNIETTLHN"
     gene            complement(17913..18440)
                     /locus_tag="PK754_04085"
     CDS             complement(17913..18440)
                     /locus_tag="PK754_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNB08714.1"
                     /translation="MSKTKGRMDVISKTFLDLVHTHQGIIYKICRVYAHTPDDQDDLF
                     QEVLLNAWKSYPRFEGASKFSTWLYRVALNTALMYKRKQQRSIVAERVEDGVLENMAP
                     EKSEDTEPLRRLESAITQLEPVEKALIVLYLEEVSYADMAVIMGLTESNVGVKINRIK
                     KQLKQIMEAGNHGKR"
BASE COUNT         5237 a         4633 c         3961 g         4817 t
ORIGIN      
        1 cggtgccaac gcttccaaag tcatggataa cgacgatctg attgctttgg ttattgttct
       61 tagaaatatt acgaaagaaa aagaaattga tcggatgaaa accgaactgg tgaatgtcgt
      121 cgcccatgaa ctgcgctcac cgttgaccag tatcgccggt ttcagtgaaa tcatcaaaga
      181 tccgcattta tcggaagcca ttcgtaaaga atacattgat attatccact acgaatccgg
      241 tcgtttagct gaaatgattt ccaagtttct ggacatcagt cgcattgaaa gcggcaaaac
      301 ggttatcaat aaagtcccat gcgatcttac cacggttgta gataacacca tcaccgccaa
      361 cacaccgatg gctcaaaagc gcagcatgca cgtcagcagc acattgccca aatgggttcc
      421 tctggttcac gccgatcctg atctgatcgg tcaggtcgta ctcaatttct tttccaatgc
      481 cgtgaaatac agccccgaag gttcagaaat caagatgcgc gtcgcggtcg cggatgatac
      541 ggtgatcgtg gaagtgtccg ataacggcta cggtatatcc aaagagaata tgaaacatct
      601 gtttcagaaa ttttttcgcg ctaaagatga gaaacatgtg aaagatgtgg aaggcaccgg
      661 tttgggattg gcgttcgtca aagaaatcat ccatcagcac aatggtacca tacatgtcga
      721 gagcgaagtc ggaaaaggtt cttctttttc gtttgccctg ccgattgatt ttacactgcc
      781 ggaaaatgct gcgctcaaac ccgcctcatg atatcttttt taagcaatga actcctgatc
      841 tttgtattgc aactgataca acctgtaata taatccaccc atagccaata attcttcatg
      901 cgttccgcgc tcacggattc gccccttgtg taaaaccaga atctgatcca cgtgtctgat
      961 ggttgacaga cggtgggcaa taataaccga cgtgcgtcct tgcatcagtt gggctatggc
     1021 tttttgtatc aggcgttccg tttcggtatc gatacttgat gttgcttcat ccaaaatcaa
     1081 tatccgcgga tcaaaaacca acgcgcgcgc catcgcaatc aattggcgct ggcctaccga
     1141 aagcgtgctg ccgcgctctt gcacgggttc aagatattgt aaaggaagcc ggcgaataaa
     1201 atcatccgca ccgactcgtc gcgcggcttc ttcgacctgc gcttcactaa tatcatgacg
     1261 acctagacgt atattgtcta aaatcgtacc cgaaaataaa aatacatcct gtaacaccac
     1321 ggccatcgtg cgacgcaaag catattgatc catccggcga atatccgtct gatccagtaa
     1381 aatatcgccg cgctgagctt cataaaaccg cgacgcaagg ttgataatcg tacttttgcc
     1441 cgaacccgtc gcaccgacca atgcaacact ttgaccgggc tgagtaacaa acgaaatatc
     1501 ttttaaaata tagtcttcat ccgagcgtat atcctgagga ttataagcaa aatacacatt
     1561 acgaaattca atgcgtcctg acatcacttt cggcgttatc ggttgagccg gattaggtat
     1621 ttccgtcggt gtgtctatga gtttgaaaac acgctccgac gaagccatcg ctgtctgcag
     1681 aatattgtat ttatcactca gatcctgaat cggcctaaaa aacaacatgc cgtactgtat
     1741 aaaagcaatc aatgtaccca gtgttattga cgaagagcgc acttctccgc ttccatacca
     1801 aatcaacatg ccgatggcaa aactactcgc cacgttgatc gccggataaa accaggcgta
     1861 atgaaatacc gaatccaaat gtgaatccgt gagcgtacga ttgagcgcat caaacttttc
     1921 aaaatttttc ttctcacgat taaaaatttg aaccgtggtg acgccggtga tattttcctg
     1981 aagatacgaa tttaaagctg cgattttaag acgcacttcg cgaaacgcga cccgtacctt
     2041 ggacttgaac acaacggata tatagaatat gagtggcaaa atggccagcg taacgagtgt
     2101 caaatgtaca tcgagaacaa acatcatgac aataatgccg gccaataaaa aaatatcacc
     2161 gaaaagatag acaagcccgg aagtaaacat atcgttaagc gattcgatat ccgatgttac
     2221 gcgtgtgata agtcggccca cggggtttcg atcaaaaaat tgcaaatgaa gatgctcgag
     2281 atgcgtaaat aatttcatcc gcagatcaaa aatgatacgc tgacccatca gttgagtcac
     2341 atagatcaca cagtattgta aaagaaattc gccgatcaaa agaacgacaa acaagatggc
     2401 aaaattgcgt aaacgtaatc cgtcaccggc cagtatagcc gcgtccacgg cttcctgaac
     2461 aagtttggga cggtatgcgc taaaaccctg agcggctatt aataaaatca cagaaacaac
     2521 ggccatccat cgatacgggc gaaccaaact caatagccta cgcatcaaac gcgcatcgta
     2581 ggctttcccc atgatatcat catcttgtgt ctgcatcgtg tattattgtc tttcgtaatg
     2641 atgtaataat ttttggatcg gcggacgtat tagagcctcg tttcctataa ttgctaaaac
     2701 ccaatgtttg ggatcaatca tatcagcggc ttttctcaga tcatcctgct ctaacgattg
     2761 taactcggcc aattcgcgtt caacgtaatc cggcggtaaa tcaaaaaaag cgccttgctc
     2821 gatcaataaa gccatgcgct caatggtttc catcgtaaac ggatgatgtc ctgaaaaata
     2881 aagtttggca tcctcgagtt cctgatccga cacgccatcg gctttgatgg cttccagttc
     2941 ctttacaata gccgtaacgg cgcgaggcgc gtgagccgcg gaagtctgga gtacgacaga
     3001 aaacatacca ccgaatttac gttttgtaag actggaatat acggaatatg ccaaaccttg
     3061 ttgcgtccgg atatgatggg ttaatcggga cgataaaccg gcgccgccta gaatataatt
     3121 tagtaccatc tgcggcacat ggcccggctc accgcgtgcg gggccggtat ggcctagcgc
     3181 aatatgcgcc tgtgcgatcg gacggtcggt caaacaaaaa atacgttgtc ctaatggttg
     3241 ggggctatcc atatgaatcg cgtttatatc tattgaattt ttaaaatggg aagcccagtt
     3301 attttcaaca aacgttctca ttttattttc atccacatca ccgaccaccg tcacgatcgc
     3361 accgcacgcg gtgagctttt cgttataaaa tcgcagcgct ccatcgcgcg ataaatcatc
     3421 cacaccgctt tccatgccat cggtcgagcg gccataggga tgtctaccaa ataacgtact
     3481 ttggaataat tcacccaata aaaccccggg catatctaat ttctttttac aatcggcttt
     3541 catttgccct cgtgcacgtt gcaactcgtg ttccgggaaa acagcatccg taagaatttc
     3601 atgcacgatc aaaagcgccg gctcaaattg ctcacggctt acggttagat gtaatgtaaa
     3661 cgcatcatgg gtaactgata cggagagcga agccgccaga tattctagtg catcggctat
     3721 actcagagcg gtttgtttgg atgtgccgga ttttagcgtc agcgcaagta acttacacag
     3781 accgggttgg tcggccggtt catgcgcgat accgacagga aatcgcaagc gcaaaaccac
     3841 aaaaggcgtg tgtgtggatt gacgaaggtg aagcgtccac cgattggata gagtgtggga
     3901 cacggatccg acgaaattag aaagcggatt gaattcggac acttctaatc gcgcggattg
     3961 gtaaatttga gcgatttata atcttgtccg acgatgacgg taacctcgag catcaactgt
     4021 gggtttttct gctgaataat gcccgactcg ccgacaccaa gcgcgacggc gacttgttgt
     4081 gcaccggcta aatttccagc ccgatcctgt acgattgttt ttttgtaatt ggaattcggc
     4141 gcattgcccg tactcacaac atcaaaccct ttactgcgta gatagtcggc cgctttggcg
     4201 gccagtttgg gtacgccgct gccgttgagc acttcgatac ggattttctg aggacgtgat
     4261 tgggtaccat caaacgtggt tgacggtgaa gtctccgcag gcacttcatt ggtctgtgtt
     4321 tcggcttctg atgtgccgct gctattgata cgaataacca acgaaatcac gagaattaaa
     4381 acgacaaggc ccaaaccgcc aatcatgtaa ttcaaaattt tgtcccactt gggatcgtga
     4441 ataccggtca tcgtcagttg ggtcggctcc ggcttcggat cttgagttcg ggttgccgat
     4501 gtttgatcca aagtctgtgt tgattctttc ggacgatcgt ccgcgcgcac tttgcgttgc
     4561 atatccactc cgtaaacaat taatcccgac ggtatcggga ttaaaggtat tttttaaggc
     4621 ctcataaatc cgtaatcgtc aaacaatcct cccgaaccgc ttcgttgtgg acccggatta
     4681 aaaagactat taccggtata ataaggattg ttactgtatc ccacttgcat ctgaaaatgt
     4741 cggctcggtc tataaaccaa ttctgctccg tgcaatattt cacggttttt ttgcattaga
     4801 ctattcttat tctgaccgaa cgactgatgc atcaatcccg tattgagacg cagcaccaaa
     4861 ggatccgaga gacgaatatt aagcgaattg gaatacatac ccagagtacc gctcgcgccg
     4921 ccaccgctat aatacgtcat ggcataactc tgcgtcatcg tcacacggtc ggaaccgata
     4981 aaactgagaa gcgatccggg aacggctgaa gtaatcccgc catgcacatc aatgggttca
     5041 cgcccagtct gtgttttgag ttgcgcggaa agcgaaactg aacaaagccc gaaaatcaaa
     5101 aggaatatcg tttgtttttt catgctaacc ctttctacgc ttagctgaat caatatcaaa
     5161 aaaaggtata aaaaagtcaa gagaacgcag ctcggttttt atggtacctc gatcacgtac
     5221 ccgatgccgt aaaccattcc gtcagcgata aatttttcat atacccccgt ttgaccgata
     5281 gccgattgca cttcccgcgc aattacacct tcgatcacaa cactattttc ggtgacattg
     5341 ggttcgtaat aactaacgtc acgcgtatag acggaggacg gtaaaaccgc acgcacaggc
     5401 cacacaggcc attgcgcacc gcgcgctgaa cccgattttt tcacatagag tcggatacgt
     5461 gatgccacat aaggacggag cgcctcatat tcccatgtat ccatttcttg attataataa
     5521 tgcatactat ccaaccaacc gccttgaact gaaaatccaa gccctcgcac agcgagcgta
     5581 tccgaacctg tgatccactc tatcactgga atcgccgcag acgtatccgc agttttgcga
     5641 tgcatagcct tcacagaatc cctgatgttt tgtatgtgtt ttatacgttc acgaaacact
     5701 tgcggtttca tatgaccgag catccagcgg cgtttacctt cgttatatgc ggggcgtatt
     5761 acaacaccgt tggcataaat tgtcaaagat ggcgcattga gttcctcgcc ggtccaagag
     5821 gccgcaccgc cgcgagcccg tttggaaaaa acaatcggac tctccggttc aatcaaaaca
     5881 tgccgcgatg cgcatcccac aacactaaac aaaaaaataa gactgcctac aaatttaacc
     5941 atgaaacggc tccaccgcga tatcaagcaa ttgtctgaaa tcggtcaatt cgtaatccgc
     6001 accggattcg cgtgtatcaa atgtatcgcc gtatcgggca aatgccgttt tcattccgat
     6061 ctgtttggca ccggccatat cgcgttccgc ccaatcaccg accatcaaag cgtgctccgg
     6121 cgggatatcc atctgcgtta gcgctttgcg aaacggctcc ggattaggtt tacgctgaaa
     6181 cgtatcttcg tacgtcacga cggcatcaaa ggtatgatga aggcgcagat aacaaaggcg
     6241 cagccacgct tcacgcgtcg gcgcatcact gacaacggcc aatttaaagc gacgtttgac
     6301 tagttcattg agcgtagata aaacatgcgg ataggtgacc agcgaagctt cgcgggtctg
     6361 gcgatacgcc acaataccgt tggctaaaat tttgtggtca atgcggccga gcgcattctc
     6421 caaaaaaata tcaaacacct tttggtattc gtagccttgc gaattataga tcgtccatat
     6481 tttggagata atatcttctt tttttacctc caagccggca tcgatcatgg cttccgccgc
     6541 actggcaatc gcacgttctt tgaggcgcat aaaatcaaca agggtattgt cgagatcaaa
     6601 aataatcgcc ctcagatgga tatcgggttt caccatacaa aatccattta gtgtatttcg
     6661 taattttttt tgcgcatggc cacatttaat acaaatccga tcaggaacat attggcccaa
     6721 agcgccgaac cgccataact caataaaggt aagggaatac ccgtaaccgg cataagacct
     6781 gtggtcatgc cgatattgac aagcaactga aacgcaaaca gcgacgcaat tccgattaat
     6841 gcgaggctgg caaatttatc atcaaccgca tcggcaataa tgacaattcg aaaaataagg
     6901 agaccgaata acgccaacaa aaatagactt cctaaaaatc cgaactcttc accgatgacc
     6961 gaaaaaataa aatccgtatg ttgttccggc aaaaaattga gctgtgtttg tgtgccgtga
     7021 agataccctt taccgaccaa gccgcctgaa ccgatagcaa tcttggattg tgtaacctga
     7081 taaccactgc cttgcatgtc acgttccggt tctatgaaat tgaggatacg ctgcttttga
     7141 taatctttga gcgattccca aatcggttcc gatgaaagtc ccacaatcac attgacggca
     7201 aaaacgccaa ggatgatcag tgtttttcgt tttgaaaaat acagtatggt cagaataata
     7261 aacagcatcg taataaacgc attaaaactg taaccgctga tgatgtgcac cagtacggtc
     7321 gcaatcggcg aaaacataac gaagatggaa aagagcgaaa taccgttcca atacaggata
     7381 ggcaatgcta cgctgcccac gcaaagcgcc gtacccagat cgggctgctt cataactaac
     7441 agcatcggta tcgccgcaac ggaaaacacg ataataatat tcatcacttg cgaactgttg
     7501 acttgccgca atgccaatat ccgggctata aaaagaatgg ctgcaatttt ggcaagctcc
     7561 gacggttgga atccgaatgg gccaagagta aaccagcgcc gagcgccata acgtaccgaa
     7621 ccggcaaaaa aaactaaaat aagaagcgct atggtgactg cgaaaacagt aaacgaggcg
     7681 ccatctatca atcgcaacgg gataagcacc gtcatgagca tcagtacgac acctataaaa
     7741 aagaacgtga gttgtttggt aaagtttaat tgatcactcg taaacgtcgc actgtaaata
     7801 cacgccaatc ctaaaccgca caacatcaat gcgggcgcaa gaatcagata atccaagtcc
     7861 tgtaaaaatt ttttaattcg ttgtaccatg aacttcgact acggccgctt gcggggctgt
     7921 agtattttta ggcgtttctt tgcgatcttt ttctttcgga tcaaacgatt ttttattccg
     7981 aacaaaattg aaataataat tcatcacaag cgcactcatc ggcgccgcca catcggaacc
     8041 ctcgccgccg ttttccataa gcaccactat agctatttcg ggattttcat acggcgcaaa
     8101 acctacaaac cagccgtgat aaccttcggt accggaaccg actttaccgt gcacgttctc
     8161 ggctgttcct gttttaccgg cgacctgcgc cgctttcaat cccgcttttt ttcccgtacc
     8221 ttcaaaataa ttgacgacac cgaacatcgc ttgccgcatc atatccatgt gctcttgttt
     8281 taccgccaca tgtttgaccg ggaaggttac acggcggata ccttcactat cctccgcata
     8341 ccgcaccaaa tgcggcgttt ggtaagtacc ggcattcgca aaaatcatcg cgtattgcaa
     8401 aagttgcacg ggtgtcacta gaatttctcc ctgaccgata cccagattga ctaccgcgcc
     8461 gcctttccaa ttattgaaac cataacggct gttgtagtat tctgtcgtag gaatcaatcc
     8521 tcggcgttca ttgggaatat ccgttcctgt aggctcacct agaccaaaca tacgcgcata
     8581 tttagccaaa cggttgatcc ccaatttcca tgctaattca taaaaataaa catcgcacga
     8641 acgcaccaat gaactggaaa cattcatgtg accgtgacct ttaagattcc agcatcgaaa
     8701 tttattaccg ccgagctgat acacgcccgt acactccaca tacgtttccg gtgttaccaa
     8761 tccctcttcc aacccggcca atgccgtgat cattttgaag gtagagccgg gcgcataccc
     8821 gctttgtacc atacgattaa aaagcggttt gtcgggattt tcattgagtt cgcgccattg
     8881 atcaatcgta atgccatcca caaaccaacg aaaatcataa tccggtttgc tggtggccgc
     8941 caaaatttcg cccgttcgtg tatcggccac catgatcgaa ccacgacgcg tgcctagagc
     9001 gctttcgaca aatttttgat aatcattgtc tatcgagaga tacagattat aaccgtcgcg
     9061 cgggtgtttg gatatgccga gatcacgcag aacttgaccc ttggcgttgg tttctaatcg
     9121 atgatatcct ttttcgccac gcaattcgac ttcgtaaaac ttctccaatc ccgatacgcc
     9181 gacacgctcg ccgattttat agtcggtatc ctgaaactcg gccgttttat aataaccgat
     9241 agtattatcc gtaatctggc tcatatagcc gatcagatgc ggcatggtga tgtcgtcggc
     9301 ataaatccga ttgatatcta ctttatatcc gatgccacga tgaaaacgtc gctcttcttc
     9361 aagatgtgcg atcgtgacaa aatccacttc cggttttatt acgaccggcg cgtagggcca
     9421 tgcgccgcgc agttttttga gaatgtcctc atccgtcata ccggtcagcg aacgcacata
     9481 ttgcatttcg ccggtgctga gcgtgtcaaa ttccgacaag gtaatcgtca atgcaaacgt
     9541 aggccgattt tcaacaatgc gccgcccata tcgatcgtaa atcaatccgc gcgagggaat
     9601 ctgatcaatc gtgcgggtgc tgttatcttc ggcacgcgcg tgaaaattat cataagccat
     9661 gatttgtaaa taaaacaaac ggccgagcag catcagcaaa atagccgcta acgaaacata
     9721 catggtaacg acacgatgat gattcatagt ttattaaaaa tcataaatag tgttgaaacg
     9781 aacatagtcc agtgtgcgct ggggtatgga aagataaatc acgaaggcgg tgacggccga
     9841 atatagtgaa ttccataatc catagtgaaa catcaggctc cagaaatcta cgacgccgtc
     9901 cagcgattgg atcgcataga aaaacacggc atgaataacc gatagaaaaa aaatcatgat
     9961 cgggaaataa tatttttcga tcagcatcac gcgttcggcc gcgaagaagt atgtcgcaaa
    10021 ggcggcaatt gttttacata acgcattgag tccgtaaaaa tcaatcatcg catcttgcca
    10081 aagcccaacc gtaaatccaa ataacatacc cgggatctgc ccgaggcgca tgcttacgaa
    10141 taccgtcaat atcagaagca aatccggcac attatccccg acggccagaa aaactgaaac
    10201 aaaactgact tgcataaggt aaaaaaaagc cgccgcaccc agtagcgaga atgtcaaaat
    10261 atttcgttta cgacgcatag atcaacgctt tacgataaaa taattttcaa atcctacagc
    10321 caccgaacgc gttcgggacg tatccgttac gacaaacact tcttccaaac gatcgaaatc
    10381 aacggccgtt tgcacccgga tatctttgaa aagaccttcg atctcgttat tcacttccgt
    10441 aaccacgccg acacgaaaat tgggaagaaa atattcgcta tactccgacg tcaggatgac
    10501 atcgccgata cgcacatcca gatttttaag aacgccataa aatgccactt catccggatt
    10561 gccgggccaa aataccactc cgttggcacg ggtacgctgt atttttgagg caacacgaaa
    10621 atttttatcc aacagcatct ggcaaacgga atgctcttca aatacttcgg cgatctggcc
    10681 gacaagaccg tattcgttga tcacattttg gtgcagccgt ataccatcgg cagaaccttt
    10741 atccaaaaca atattactga tcgcagggcg cgactcccgc gcaatgatgc gtgcgggacg
    10801 cacactcagc ggaaaccggc ttcgaaattc gaccaactgg cgcaggcgta cgttttccat
    10861 atacgcatct tccaaaacga tattacgcga cgccaattcg atattgcgct gttgtaattc
    10921 cttaataacg gcatccgaat cgcgccaccg cggcccggat gaaatttttt cacccagcat
    10981 cgccgcgaga taaatcacgc gtttgcggag aaaaatcatc gattgattgg gattattctg
    11041 caaaagaaga atagatgcga tgacaagaac gagaaaaaca aatgaatgcc ggtagacata
    11101 caggatctct ttcaggccgg cataaaagtt tcttagtttc cagagcatga tgggatctta
    11161 atgcacgcta ccggtacggt acggcgatta gtatttgcga tttttgagaa tgacggcctg
    11221 atatttggtc aggttttcca aaacgcgtcc gacaccgcgc acgacggcgg tcagcggatc
    11281 ttcggcgacg atcacgggta aacccgtttc tttgcgcata cgctcatcga gccctttgat
    11341 caaagcgcca ccgccggtaa gaacaatacc gcggtcaatc aggtccgatg ccaattctgg
    11401 cggagttttt tccagtgtac ttttcgtacc ttccacgatt tcacgaatcg ttccttccag
    11461 tgcgcggcgt acttcttcgg aatccgtttt catcgttttg ggaataccgg atacaatatc
    11521 gcgacctttg atttccattt tcaattcctg cgcaagcggt acggcggaac cgatattgca
    11581 tttgatatct tccgccattt gttcacccaa aagtaaattg tgctggacgc ggaaatatgt
    11641 gataatagat ttagtcagtt catcgccggc gctgcgaatc gaacggtcgc atacgatgcc
    11701 cgacaatgca atcacggcga tttcggccgt tcctccgccg atatcaatca ccatactgcc
    11761 tttggaagct tcgatatcta tacccacgcc gatcgcagcc gccataggtt ccgccacaag
    11821 atatacttct ttcgcaccgg cgtgttcagc cgaatcgcgc acggcacgtt gctcgaccat
    11881 tgtaatgccc gaaggtacac cgacaactac gcgtttaccc ataaaccggg ccgggctcac
    11941 tttgcgaata aattcctgca gcatcagttc ggtcatttcg aaatcagcga tcactccgtc
    12001 tttcagcgga cgcaccgtaa gtaactcgtc atgggtacgc ccttccattt ctttggcttc
    12061 tttgccgatc gccatcattt tattggagcg cttattgatc gcaacgatcg aaggctcatt
    12121 gatcacgacg cctttgtctt tgacatagac caacgtgttg gccgtcccga ggtcaatcgc
    12181 aatttcattg ttaaaaatat caaacaggct cataacgctc cgtatgcgtg gttttcttaa
    12241 atggatctaa tgtttgaaat gacggatact tgtaaatacc atcgccatat tgtgttcatc
    12301 cgccgccgcg atgacttccg catccttcac ggaaccgccc ggctggatca cggccgtagc
    12361 acccgccgcc gccgccgcgt caacgccgtc gcgaaaagga aaaaaagcat ccgacgcgat
    12421 ggccgaattt ttcagcgaaa gcccgctttt gccggatttg ctgacggcta actccgacgc
    12481 atcaacccgg gacatttgcc cggcaccgat agcgagagtt ctgtcagccg cagcataaac
    12541 gatggcgttg gacttaacgt gtttgacgat cgtccatccg aaaagcaacg cgtgcatttc
    12601 ttccgcattg ggagcgcgtc gcgttacgac cttccactcc gtctgccccg cacgacgcat
    12661 atcggcgtcc tgaatcaaat aaccatccaa cgctttgcgg atctccggca acgccgaccc
    12721 ctgcgcccgc gccgcttcaa aatcataacg gattaaccgg atattttttt tctttgaaag
    12781 aatctctatt gcacccgaat caaattccgg tgcgatgatc gcttccacaa acacttcact
    12841 taaggcttcg gcggtcgccg cattgagacg gcgattaaac ccgatcactc cgccaaatgc
    12901 cgaaaccgga tctgtagccc tggcattctg atacgcctgg tacaaatcgt cgtgcatcgc
    12961 ggcaccgcac ggattggtat gtttgacgat aacgcatgat ggtttttcaa aatccgatac
    13021 aacgcgcaca caggcatcaa gatcgagaat attgttgtat gacaattcct tgccgtgcaa
    13081 ctgatcataa tactttttag agtgaaaatt agccgcgtaa aatgacgctt tttgatgcgg
    13141 attttcaccg tagcgaagcg gctgaatttg cttaaacgta agatttattt tttcgggcaa
    13201 cgtttccgaa gaagcggtta ctttagatgc aaaataactt gaaatcaatg catcataacg
    13261 tgccgtatgt tcaaacgctt tgatcatcaa acgataacgg gtgtcttgcg ccacatcgcc
    13321 gctagtcatg atttccttca tgacaggttc gtaatccgat ggatctacca gtacgatgac
    13381 actgcggtaa ttcttagaag ccgaacgtat catcgaaggc ccgccgatat caatattttc
    13441 gatagcctca tgcaaaggta catcgggttt gctgatcgtc gcttcgaaag gatataggtt
    13501 aaccgccaaa agatcgatca tgccgatgcc gtggcgatta gcctcttcca tgtgggtagc
    13561 gttatcgcgc aacgccaaaa gtccgccatg tatcttaggg tgcagtgttt tgacgcggcc
    13621 atccatcatt tcgggaaatt gcgtgagatc tgaaattttt acgaccggaa ttccgttttg
    13681 agaaagcgcc gacgccgtac cgccggttga aataatttcg acaccggctt gatgcagttt
    13741 ttgagccagc gattgaattc ctgttttatc ggatacactg ataagcgcac gtcgaatcat
    13801 cttggaagca acacttctat aaataaaata taatttttcg ggcgcgccaa tgtagaaact
    13861 gagcggttaa aatgcaaggt gtttagaggt gaaattcatt tgataagagc atggaaacat
    13921 agacatactt attgcaaacc ctctcattga taaaacaaaa aaggaagagt tttagctctt
    13981 ccttttttat acgattcctt atacctacta ttgagcggtc gccgtaaatt cgccggactg
    14041 caacacttta ccggtgctgt ccacgatttc gactttccag gtaccggcac ggtgcacttc
    14101 ataaaacgca tacgtgcgca ttgaccaaga tttgatttcg agcggtactt catttttcaa
    14161 ggtttcgccg tgataccatt tcatggtaac cgtctggcct tcaccaccta caactttgat
    14221 ccagcaatat acattgctgc cattggcggg aaacgatgta gcttcgccga caacttcgct
    14281 tttttcgaca gcagtgccaa acttcatacc gtcaactttc atctgcgaat aaccggtttg
    14341 aacaaatgcc aaagcgatta aaacgaccaa agcacttacg tacttcatac tacacctctt
    14401 tttaaaatta ataaaataaa tatcgcgagt ttttcccact tcgcatgcct attatacacg
    14461 aaagcgctta cgtggtcaat atttaaatat aaaaacctac gaaaaaatcg caggttttaa
    14521 cggtaacaga ctatttttta aaattttatc tcgatccggc gccccattcg taatttagat
    14581 ccaaacgaaa tgaaacctct tttttatctt cttccgaagg attgtcaaca atcaaataat
    14641 acaatgtttt ttgggcgcga aattgattaa gtagccgaat tttaaatgtg tgttgtattt
    14701 tttcgccgga atcgtattgt gttttgtaag gttcttcttc tttaaatttg agaaaatttt
    14761 cttcgtccat caccatcaca cgcgctttgc gatcgccacc gtcttcgaca cgccattcgc
    14821 catctacttc gatcgcttcc atcttatctt ccagtgtaat ttcaaattga taatactcac
    14881 cttttcccag tgtttctttc ttttcgacat gtgtgatttt actgttcatc acggctttat
    14941 tactaccgca gccaaactgc atcacgaccg ccaacatcgc tatgccataa agccaaattt
    15001 tacttaaacg catacatttc cctttctgaa ataataaaag ttattgacct cgcatacatc
    15061 agtataccta ttttgaggta aacgatgatt catcctttat aaaacatcca tttggctaat
    15121 tcgggcaatg tgattttttt accgtacatg agaattccga tacgatatat gcgaccggct
    15181 acccacacgg cgctcaaaaa accggcaatc agcaagctca tcgaaagcgc caattgccac
    15241 caaggcacac caaaaggtat tcgtcccaac attacaatcg gtgatgaaaa cggaatcatc
    15301 gacgcccaaa atgccaccgg cccgtgcgga tcattgaccg aaacctgaag cagaaaaaaa
    15361 gataaaatca ccggtatggt gacgggaaat gataacgact gcacgtcacc ggcatcattg
    15421 gcgcccgagg ccgcggcggc gtacaatgcc gcataaaaaa gataaccgcc gagaaaataa
    15481 aacacgaagc agaaaaataa ttctgtaaaa ttaattgaat ccagattttt cataaatacg
    15541 gttatcgcct ccgcatcgcg ggctttgata ccgccttggg caagtgcatc cggtgaaaac
    15601 tgccccatct gcggtcccag cagcagcata gccccctgaa acagtagcgc gccgatagct
    15661 atccatgaca cgagctgagt caatccgacg gcggcgacgc cgaggatctt acccgttaac
    15721 atttcaaaag gccgcacgga cgagacaatc acttccacga tgcgattggc tttttcctcc
    15781 accacaccat tcatcaccat cacgccataa atgataagaa tgatataaat aatgataccc
    15841 ataataatcg ctgtcgtaag agcgacaact ttattatcgg aagactcacc ttcttcggtc
    15901 ataatgatcg tttcaaacga aacacgctgt ttgatcgcat cgatcttatc ctttgtcatt
    15961 ccagcctcgg ataatcgcgc tttttcctgt tcacgtgaca attcgctatt gatatataag
    16021 gttgtcgtaa acccaagacc gcgggatgaa aaatatttta tcccatcgtt tggatcggta
    16081 ctagccgcag gaatatacaa aacaccgtcg taattatatt tggtgtcccg gtacacacgt
    16141 ttgagcgaat ctaaaggcat ggattcgtag aaaaactgaa caagccgtga atcctgcaat
    16201 ttctgaatat ataagccgct ttcgtctcgt acggcaatgc gttcggcatc gttattgcgc
    16261 gcgatcagaa atatcgtcac cggtagaaga agcatcaaaa aaggtacaac taatgtcgta
    16321 atcaaaaacg atcgtttggt cacgcggctg atatattcac gttttaaaac aagtcctatt
    16381 ttacgcatga ttgtatccct tcacatgttc gatgaaaatt tcattgagcg acggtaaaac
    16441 ctcctgaaat gcatacattt ccacccggct caggaggtgc gttaataatt gattgggcga
    16501 aacgccgggt tgtgttgcga tcacggcttc acctgttttt tgcgacacga tcgaataagc
    16561 ggtatcgccg gattgcggta aaattccgtc aaactgtatg cggtagtggt gctttttgaa
    16621 ctgttgtttg atcgctttga cttccccgct gaggatattg cgacccaggt gcaccaatgc
    16681 aatacgatcg catatttctt cgacttgctc catacggtgt gtggaaaata taatcgtcgc
    16741 tccttcgtct ttgagacgat ggatttcatc tttgatcaga tcggcgttta tgggatccag
    16801 gcccgaaaac ggttcgtcca gaatcagcaa ctcgggctca tgtacgaccg tcgcaataaa
    16861 ctgcactttt tgctgcatac ctttggataa ctcctctacc tttttattcc accagtccat
    16921 gatgtgcaga cgttcaaacc acatttgaac ttttttttgg gcatcccata acgacagccc
    16981 tcgcagctgt gcaagataca tgacatgatc gccgactttc atttgtttgt aaagacctct
    17041 ctcctccggc atgtatccga tacggcgcgt atgcgactcg tgcaaagctt ggccgtcaaa
    17101 ccggatcgcg ccttcatccg gcatggtgat ctgcgtgatc atacgtatca gcgatgtttt
    17161 cccggcacca ttgggaccga gtaaaccaaa aatggattga cgttgtacat caagactgat
    17221 gtgatccacg gccgtatgcg actgatagcg cttgactaca tcacgcacag aaagtttaac
    17281 atccgtcatc gtcatgattt ttcctcgata gtaattaatt atgtaaagtc gtttctatat
    17341 tttgcaatgc ttgtaactga gccgtcattt gttgtgcatc tttatcaaac atccagcgaa
    17401 cataggggcg ataaaaaaac gtaactacgg tgataaagcc cataaatccg agtttgaaat
    17461 acatcatata attttcgttt acggcctcca tcggtataaa caaaacaatc aatgcaagca
    17521 ccatactgaa tccgtatgcc gagcgcagat agatacgaat tgttttttga tgaagccgaa
    17581 ggtatgccgt atgacgtgcg cgcagatcta ttccgtaatc taatgatgat acacggcgaa
    17641 tcagaagaat aaacaaaaag ccattgggta tgataaacag aaaggacgcc acggtaatcg
    17701 ccaatgaaat tagtggtaga tgcgattgga caaataacac cgcgccaatc ataaaaagaa
    17761 tggccaacat tccgctcaat tcaaaaagaa gtttctttcg gatacgaagt tgcgccgttc
    17821 gaatccgcat ttccagaagt ttacgaatat ctttttcatt cataccggtt tgaggataaa
    17881 gcgactgccc ttgtttccaa agttcacgga gttcatcgtt ttccatgatt tccggcctcc
    17941 atgatttgtt tgagttgttt cttaatacga ttgattttta caccgacatt ggattccgtc
    18001 aaacccatga tcaccgccat atcggcgtag cttacctcct cgagatacaa aacaatcagc
    18061 gctttttcga cgggttcgag ctgtgttatc gccgactcca atctccgtaa cggttcagtg
    18121 tcttcacttt tttccggggc catattttcc agtacgccgt cttccacccg ctcggcgacg
    18181 atcgaacgct gctgcttacg tttgtacatc agcgccgtat tgagcgccac gcgatagagc
    18241 catgtcgaaa atttggaagc gccttcgaat cgtggatacg atttccatgc attgagcaaa
    18301 acttcctgaa aaagatcatc ctgatcatcc ggcgtgtgcg catacacccg gcatatttta
    18361 taaatgatcc cctgatgcgt atgcaccaga tcgagaaaag ttttagaaat aacgtccatc
    18421 cggcctttcg ttttgctcat aggttgccgc aaagtgcgaa aacttacaag gtttcaaaac
    18481 gaaatcgtat aaaaaagaaa accgggcatg aggaaacccg gtttatacga tatattgcag
    18541 gacgcagata tatcgcggat acatatattt taaggtaaat taaggcgtac gggatttggt
    18601 tttgaaaaaa ctcagcacgg tttccgtcgt attaaaatca ttaaaaaa
//
LOCUS       DAOQNQ010000036        18229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1079_length_18229_cov_7.556619, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000036 DAOQNQ010000000
VERSION     DAOQNQ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18229
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1079_length_18229_cov_7.556619"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..1274
                     /locus_tag="PK754_04090"
     CDS             183..1274
                     /locus_tag="PK754_04090"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB08715.1"
                     /translation="MNQPEPYSYFHALRVYVITSLRAISRNPSSIVFGFIFPVIFIVI
                     FGFIGNRTLNLTIGVIQNDSQENPMMEMLSHMDQIKLMRNPDDVALTEKLRKGEVDAL
                     MRVEKTDSGYVAHIQTSDASPNGIVVLNMMRGLTAQANLLMMQVQHVPLAIAHTTIEG
                     RRYKTIDFILPGQLGFVVLSSGIFGAAYLLIALKSRLVIKRMFATPAPKSVILVGEAI
                     ARMLFALAQVSIIVLIGKWVFGFTLINGWVTLLMMLLVCLIALMVFLGFGMIVSNIAR
                     HEQAVPPIANLIVMPQFLLAGTFFSIRVFPEWLQPVCHALPLTYVNDALRKIAFEGAG
                     FADVGFELMVLGVWGVIVYAVAVKLFKWE"
     gene            1290..>1681
                     /locus_tag="PK754_04095"
     CDS             1290..>1681
                     /locus_tag="PK754_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08716.1"
                     /translation="MKFNDTIKRFIIHEIAWAFVVICTTGFIIGALYVPVWTWNNYWI
                     SLRVSFSFYVVMRFLMGTFAERVIRRFVPRTLERPKKFFAIEILIRVILGTVGSFLAF
                     MLAEIITGINFLISLRMIYVQVFIAVILT"
     assembly_gap    1682..1781
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1782..2246
                     /locus_tag="PK754_04100"
     CDS             <1782..2246
                     /locus_tag="PK754_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB08717.1"
                     /translation="RYVLVASEKETVTLDEELTFLDNYLRIERVRFGEKLTICENVDV
                     TVRPVRVPGLILQPLVENAIKHGLAPRRENGLLEISASRQDDFLILRISDDGEGFSVD
                     RNGKVIFNREGVGVKNVRERIEKMYGGKGQLILSNRPQGGAVAEVRIPIRVS"
     gene            2262..3023
                     /locus_tag="PK754_04105"
     CDS             2262..3023
                     /locus_tag="PK754_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNB08718.1"
                     /translation="MLIDDESLARQRVRDMLSVSFAGQIAIMDEASDGIEALEKIESL
                     QPDFIILDIQMPGLSGFEVLMHLKKVPPVIFATAYDHYAIRAFDVNAVDYLLKPFDEE
                     RLKRAIERVIAHLTVPESANTLSIQKLITDVMQEKNTQTAYLERIPYRDGEKIRFIKA
                     MQCLWFDTQDSVTYLHTDQSAHDIRYTLDEIEQRLRPSEFLRVHRATIVNLNAIRELT
                     PWFNGAYKIILNDKKNSEIDVARGRVQALRDVLGW"
     gene            complement(3074..4060)
                     /locus_tag="PK754_04110"
     CDS             complement(3074..4060)
                     /locus_tag="PK754_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08719.1"
                     /translation="MIVLTDAYLNPVWYKAIGSEKADYGTDITETSDGNFLAVGTIQI
                     TGNNKDVVTLLLDRSGKLLASKSYGGLAPDEAQRLIKLDYGVFAMIGNTGSFGHGASD
                     VWLILLDAFGDSTRSYTFGTEDDEHVLGAIQSQNGSIYCTGYVSAAEYVPFLLTINSD
                     MSNHLWVRYPARNSRDEAYDIKETSNGDLIMVGRTNIFRSPYENYDAQVFKTDASGAK
                     SWVTRFGKTKYDILESCIPLDDNGCIVIGSSLHVSPKYVANQQALIARIQSTGDTVWT
                     RQIGGSPPDEAVCILPIGLNRFLIGGNTRNGQYGDWDIVFFMIDENGNHDFN"
     gene            4380..5855
                     /locus_tag="PK754_04115"
     CDS             4380..5855
                     /locus_tag="PK754_04115"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HNB08720.1"
                     /translation="MKRSQKKLLFLGTFLCVVILLIPESELKIDTTSFDHSFEWNQDS
                     LWQTLEREFVASRYEEGLQTVKLIQVSMAQLQRMTDTLNTTTVNTSDELLWRIERSVY
                     YLATRVAVKPEFVADFSRIIMNLRFVVKKQSRNWDMKDRITRERLYRILYGMRTALEE
                     VLLQSQTDKFAEINPGHAESSVTPHADVLGVMLHSGDILVSRGGAPTSALIARGNDFP
                     GNFSHVALVHIDSVTGKISLIEAHIEKGVAIATWNEYIADKKLRILILRPRHDIPVLA
                     SDPMAPHKAADFALQLALQKHIPYDFVMNYRDHGALFCSEVASSAYESVGISLWMGIS
                     RITSPGTKQWLSYFGVRHFETQEPADLEYDPQLQVVAEWRNASALLQDQLDNAVIDIM
                     LEDADAGKPIEYNMWYLPAARLAKTYSVLLNFFGKVGPIPEGMSATAALRNQWFSERH
                     HAIRAILAEEVKKFREKKQYFPPLWERHRLAREAKNKLDMN"
     gene            5998..6555
                     /locus_tag="PK754_04120"
     CDS             5998..6555
                     /locus_tag="PK754_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019991170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HNB08721.1"
                     /translation="MKRLMMLLVITGIVLSGCQSQSKPSDNSDKGSANMPAGMSAVND
                     NVSQKNIVQIALGSKDHTTLVEALKASGLVDVLANPGPFTVFAPTNAAFEKLPKGTVE
                     ELLKPEKKSALKNVLFYHVNVSIFKAESFRDGQVMNMFSGQPATFSVKDGKVTINGNA
                     TIIASIPASNGIIHVVDGVMLAPAK"
     gene            6754..7953
                     /locus_tag="PK754_04125"
     CDS             6754..7953
                     /locus_tag="PK754_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB08722.1"
                     /translation="MSFDPMHAIQDHWVFGEYGDVNPSITDSSTYTFMSPDKMRELFD
                     HEIEGCFLYSRHWNPINKELSRSLAALEASASAQVTASGMAAISSSILQSCMTGDEVI
                     CSRTVYGGTYALFKNFLPRLGIKVHFANIQDIPAIQKLISSKTRVIYTEAVSNPLLEV
                     ADIPSLAKLAHDRSAKLVVDNTFSPLTISPIRHGADVVVHSLTKFINGTSDCVAGCIC
                     ASDEFIHQLTDINSGACMLMGPVLDSMRAASILKNLHSLHIRIQQHSRNALYIASELE
                     KLGLRVYYPGLVSHRQHTLLKSMMNAQFGFGGMVAIDAGTSEKAEALMTRMQQEKVGY
                     LAVSLGYFKTLFSAPGHSTSSEIPEEERQAMGLGDGLVRMSVGLDSDIGRSFARIKTC
                     LAELKLI"
     gene            8003..8233
                     /locus_tag="PK754_04130"
     CDS             8003..8233
                     /locus_tag="PK754_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08723.1"
                     /translation="MNKKMIITIAAIAVIAVGAVYAYRMNTSETCPPGMTGGDCCKST
                     EVKAQEVKSSEQQATASTETHSCCPSGAEKKQ"
     gene            8946..13082
                     /locus_tag="PK754_04135"
     CDS             8946..13082
                     /locus_tag="PK754_04135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNB08724.1"
                     /translation="MRYGLQKPFFWLLSAFVIAGILIVGSSEQRSTANMKKKHLKNTP
                     EALKQALLEQKIKRRAEGYAKPDQPEKFDILHREIRTQYGKQLPDYQMGYKNIEMEKA
                     GIIRREGEKYVRNQMMSSSTLSWTSRGPSNFLGRSRGLVVDPDDANHLTYYLGSVGGG
                     IWKTTNGGSYWENLTPEFPNLSTTVIAMASSNHNIMYAGTGEGYQAAVGMIKGDGIFR
                     STDRGASWAQLSATSSNSDFAYVNRLIIDPNNSDIVVAGTNSAIMRTTDGGTNWTKVH
                     TAGNNRIQQVLADPSDFNIQYATAEALGFLKSTDAGITWAPSSTGIGAVDRLEMAIAP
                     SNTNLLYASVESGTNSVLYRSTNGGANWATVADTTGQSASSIAWLGAQGWYDNTIAVH
                     PYDPNILFVGGINIYRINISNSISAGRRGTTRMTNWYPGLSRPSGGTYPYIHADQHNI
                     VMIPISEPNNFWILHTNDGGFARSTDGGQTWLFDEDEAKGIVTAQFYGVDKMPGASAY
                     IGGTQDNGTWMSPVDATVDSSWYAMLGGDGYDGVWKANDPNQIIGCLYDNRLYKTTDG
                     GQTWNSATSGLLDVDNANSAFVTEIGRSKLDPDLLIVPGGSGFWRSEDFGDSWTLAPI
                     TSSYGFSTARNLAEISLADPNIVWAGTNMSAAGKLHVSTNAGKSYTATNNYTAVTLGR
                     VTGIATHPYNPNEAFALFSIADKPKVLRTMDRGQTWTDISGFGANSSSNNGFPDVATY
                     SLLVMPTDTNEIWAGTEIGIFRSTDNGATWAYANNGLPAVSVWELKIIDNEIVAATHG
                     RGIWTAPISAPPLPTVVLGPTLKQAYQNPATGLVNVELGLRNTYDSTIVFMGNTRALT
                     LGSTGINDSTVSFSSTQIGSVSIKASSYRNGTLYETVAKSVTLVQAVARNDYYNAFNT
                     STSANGEFTGSLFSATKPSGFSSFALHTAHPYTNNTNNTFQLNYPITVASDSSMIVFD
                     EIAIVEPGDPGVPFPEPEFWDYVVVEATSDGISWEPVIDGWDSRANSTWLSTFNSVGS
                     GSNSMFKQRRIDLTDKYEPGTKIFIRFRMFTDAASTGWGWCIDNLKINAIETPPTVTV
                     GALASPVINVAKFAVSSNRGMSTAALTAGGQSVALSRLGGSLFFGSYSITSGTVSITA
                     TCSDSVGNTSTTLNKQFTVTPLSKSSSLGGYSVTGSGEGYVVASLVQATDIPSGWIEI
                     GSMYEAGITGTPTNLKITYDLPNESTLDRMIPGSSQRYKIGLYEKDGNQWKLISAESN
                     GKLSGEFSHKAIAAFYNPDFEGIPTEFALSQNYPNPFNPRTTIRYDVAKAGKVVIKVY
                     NMLGQEVATLINAQRDPGRYSIQWDGRNNAGSVVASGVYLYRMQVGDVVRSKRMLFLK
                     "
     gene            13112..13684
                     /locus_tag="PK754_04140"
     CDS             13112..13684
                     /locus_tag="PK754_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08725.1"
                     /translation="MKAKNKLWMICLVSIAISSCGGGGGKDKITLTKATDQFATGNYT
                     TALRNFENLIASEGAPAVAGAGWCLVRLSNVARADSFFVTVAADSNTDAYAGWSFSSW
                     ALNDPSEAISRADFVIRKDANFQMSLDTRVTKSHLVWIQASSYLQLGDYTSCFNKIKL
                     LDAAYTYPSGTSVEIATALMQKLQSLGSAS"
     gene            14032..17235
                     /locus_tag="PK754_04145"
     CDS             14032..17235
                     /locus_tag="PK754_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB08726.1"
                     /translation="MMFFDANNQEKTDFPRVLGTIYDVAYHPQYGWFIAGNFTHINGV
                     KRKYIARIKTDLTLDMNWNPIVDGEVRSIAVNEDFVYFGGNFSKVSDQTRYHIAAVGV
                     ADGVLSDWNPGADGIVYDMIVSSNTLIIGGQFTEIAGEERFSLGSIDISSGNATAWAP
                     HVTYSENNPGIVYAIVVSSNNVYIGGEFFYLGGVERQHCGAVDMASGAVNPFNPWPNT
                     IVFSLYVKNSTLYIGGMFTQIFSQVRNRLAAYSLSLNQLNSWDPNIDSYNTFPNANSS
                     VRAISVLGDKILVGGIFTKVGSTSKANYALFDLNTGNLSSITKTPSGSVFTITTASSD
                     NEAIAGGSFDSFSQYITRYRLAAFDAQTGALRDWAPVVDGAVTSIEVFENTVLIGGYF
                     NKVNNISRPYAASLIGYGATGSGQLNNWAPGLNGIVSQLLIAGESLYIRGSFTSVSEN
                     NRLNIALFDLNTMQLRQTSLQIPYPTSYLTLRAMGAVDSVLMLGIQYFEGNSFKSVLS
                     EFNTRTGQEIRNSPTNKTINAITATSSVIYLGGYFTQVKGVYRSGAAALDRKTGEIKA
                     WDAKLSGGLGLTKMVTAGNYIYMTGDFDSVGAIPQSGFTKIDMISGVCSPMGHTYNGS
                     IAGMDINEQNIYIGGSFSHFNFLSNNDFTVIQDSTILVPPKNPMCSPSLELDRVIDLS
                     WTKSSGATYYKIYRGLSSFFDTTGNCIATVGDVNTYRDTLGGYIKYYYKIGAFSEGNF
                     SKLSAIASATAPDFIAPEANIGLLVPGFGTHVKIVVTSNESLKSMIVNINNSSVTMSQ
                     SGSLFFGGYTIQQAGQLTITATAKDQSNNTTSLEKSFNVQTIGRPFYNDLYKITVSGS
                     DGYFLTTDDDKSFSGSDDLIPLQKPKTFLTTSIPSQIALAFTEYEITALSGLKKRQNF
                     TNSAIGLYAQIGDERILLAKGGDYTNSVVLEKSVQDKLISLLSTCGLTIAAFYNPDFE
                     GIPTEFALSQNYPNPFNPRTTIRYDVAKAGKVVIKVYNMLGQEVATLINAQRDPGRYS
                     IQWDGRNNAGSVVASGVYLYRMQVGDVVRSKRMLFLK"
     gene            17265..17837
                     /locus_tag="PK754_04150"
     CDS             17265..17837
                     /locus_tag="PK754_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08727.1"
                     /translation="MKAKNKLWMICLVSIAISSCGGGGGKDKITLTKATDQFATGNYT
                     TALRNFENLIASEGAPAVAGAGWCLVRLSNVARADSFFVTVAADSNTDAYAGWSFSSW
                     ALNDPSEAISRADFVIRKDANFQMSLDTRVTKSHLVWIQASSYLQLGDYTSCFNKIKL
                     LDAAYTYPSGTSVEIATALMQKLQSLGSAS"
     gene            17958..>18229
                     /locus_tag="PK754_04155"
     CDS             17958..>18229
                     /locus_tag="PK754_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08728.1"
                     /translation="MRLFLSLLVLLFAISITAQDIPPRITIYNPPGAHPQSFELTQDA
                     SGIIYAVNYNGIFGFDGVRWKKLAGRLETYDWNGITADKKTGRIYIG"
BASE COUNT         5091 a         3491 c         4244 g         5303 t
ORIGIN      
        1 ggtatcatgg atgaaggacg tatcatagcg ctggattcac cggagcgact tattcacggc
       61 ttacttgcta cggggttcca acggccgacc cgtgtacgcg aagcaacact cgaagatgta
      121 tttatacagt tgaccggcaa agaactgcgc gacgcttaaa ttttgaaaaa accgacacca
      181 tgatgaatca acccgaacct tattcctatt ttcacgcgtt gcgtgtgtat gttattacga
      241 gccttcgtgc gatcagccgc aatccgtcga gtattgtctt cggatttatt tttcccgtca
      301 ttttcatcgt cattttcggt tttataggaa atcgtacgtt gaatttgacc atcggcgtga
      361 tacaaaatga ttcgcaagaa aacccgatga tggaaatgct cagtcacatg gatcaaataa
      421 aattgatgcg caatccggat gacgtagcgc tgacagaaaa actacgcaaa ggtgaagtag
      481 atgctctgat gcgcgttgaa aaaaccgatt ccggttatgt tgcgcatatt cagacctccg
      541 acgcttcccc caatggtatc gtggtgctta atatgatgcg cggtcttacc gcacaggcca
      601 atctacttat gatgcaagtg cagcatgtgc cgcttgcgat cgcgcacacg accatagaag
      661 ggcgacgata caaaacgatt gattttattt taccgggtca gttaggattt gtggtgctta
      721 gttcgggtat tttcggcgcg gcgtatttac tcattgcgct caagtcccga ttggtgatca
      781 aacgaatgtt tgctacaccg gcgccgaaga gcgtgatttt ggtcggcgag gccattgcac
      841 ggatgctttt tgcgttggcg caagtatcca tcatcgtact catcggcaaa tgggttttcg
      901 gattcacgct catcaacgga tgggtgacac tgctgatgat gttgctcgtt tgcctcatcg
      961 cgctcatggt gtttttgggt tttggaatga tcgtttcaaa tatagcgcgc catgaacaag
     1021 ccgtcccccc catcgccaat ctgatcgtaa tgccgcagtt cttgttggcg ggtacgttct
     1081 ttagtattcg tgttttccct gaatggctac agccggtgtg ccatgcttta ccgttgacgt
     1141 atgtcaatga tgcgctgcgc aaaatagcct tcgaaggcgc aggttttgcc gacgtaggtt
     1201 ttgaattgat ggtattgggc gtttggggtg tgatcgttta tgcggtagct gtcaaattat
     1261 ttaagtggga atagaaacga gtgcccgtca tgaaattcaa cgacacgata aaacgtttta
     1321 ttattcacga gatcgcatgg gctttcgtag tcatctgtac gacgggtttt atcatcggcg
     1381 cgctctatgt gccggtgtgg acatggaata attattggat ttccctgcgc gtatcatttt
     1441 cgttttatgt tgtcatgcga tttttaatgg gcacgtttgc ggaacgggtg atacgtcggt
     1501 ttgttccgcg tactttggag cgtcccaaaa agttttttgc tattgagata cttatccgag
     1561 taatactggg aacggtggga agttttttgg cgtttatgct cgctgaaatt atcacgggta
     1621 ttaatttttt gatttcgctg cgcatgatct atgtgcaagt ttttatcgct gtcattttaa
     1681 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgttatgtg ctggtcgctt
     1801 cggaaaagga aaccgtcacg ttggacgaag agcttacttt tttggataat tatttgcgca
     1861 tcgaacgcgt tcggttcggc gaaaaactta ccatttgtga aaatgtggat gtaacggtac
     1921 gtccggtacg agtgccggga cttattttgc aacctctcgt agagaatgcg atcaaacacg
     1981 gccttgcccc gcggcgagag aatgggctat tggaaatttc ggcgtcgcgt caagacgatt
     2041 ttttaatttt acgtatttcg gatgacggcg aaggtttttc tgtggatcgc aacggtaaag
     2101 tgatttttaa tcgcgaaggc gtcggtgtaa aaaatgtacg cgaacgaatc gaaaaaatgt
     2161 acggcggcaa aggtcaattg attttgtcca accgacctca gggcggcgcc gtagccgaag
     2221 tgcgaattcc gatacgagtg agttaattta tgttccgttg tatgcttata gacgatgagt
     2281 cgctcgctcg ccaacgtgtg agggatatgc tgtcggtatc gtttgccggg caaatcgcga
     2341 tcatggacga agcatccgac ggcatcgaag cgttggaaaa aatcgaaagt ttgcaacccg
     2401 atttcattat tcttgatatt caaatgccgg gattaagcgg ttttgaagtt ttgatgcatc
     2461 tcaaaaaagt tccgccggtg atatttgcga cggcctacga tcattatgcg atccgtgcgt
     2521 ttgatgtaaa tgccgttgat tatctgctta aaccgtttga tgaagagcgg ctaaaacgtg
     2581 ctatcgaacg cgtgatcgcg catctcacag ttcccgaatc cgcaaacaca cttagcatac
     2641 aaaaactgat aaccgatgtg atgcaggaaa aaaacacgca aactgcgtat ctggaacgca
     2701 ttccgtatcg tgacggtgaa aaaattcgct ttatcaaagc catgcaatgt ttgtggtttg
     2761 acacccaaga cagcgtgacg tacctgcata cggaccaatc ggcgcatgat attcgctaca
     2821 cattagatga aatcgaacaa cgtctcaggc cgtccgaatt tttgcgtgtg catcgtgcta
     2881 cgatcgtcaa tctgaatgcg atccgcgagt tgacgccgtg gttcaacggc gcctataaaa
     2941 tcattctcaa tgataaaaag aattcggaaa ttgacgtagc gcggggacga gtgcaggcgt
     3001 taagggatgt gttaggatgg tgatttaggt caaattatga gcgcgtgata tggaagcgaa
     3061 gtaatgatgt atttcaattg aagtcatgat tgccattttc gtctatcata aaaaaaacaa
     3121 tatcccaatc gccgtactgg ccattacgag tattaccgcc gatcaaaaaa cgatttaatc
     3181 cgatcggcaa gatacaaacg gcttcatccg gtggagaacc gccgatttga cgagtccata
     3241 cggtatcacc ggtggattga attcgtgcta ttagcgcttg ttgattggcc acatacttgg
     3301 gactgacatg caggctgctg ccaatgacaa tacaaccgtt gtcatctaac ggaatacaag
     3361 attcgagtat gtcgtatttg gttttgccaa atcgcgtaac ccatgatttg gctccggatg
     3421 catctgtctt aaaaacctgc gcatcataat tttcatatgg tgatcggaat atattggttc
     3481 gaccgaccat aatgagatcg ccattggatg tctctttaat atcgtatgcc tcatcccgag
     3541 aatttctagc aggatatcgc acccaaagat ggtttgacat atctgaattg attgtaagta
     3601 agaaaggtac gtattctgca gcgcttacat aaccggtaca gtagatactt ccgttttggg
     3661 attgtatggc acccagcaca tgttcatcat cctctgtacc aaaggtatat gatcgggtac
     3721 tatcgccaaa agcatctaat aaaatcaacc agacatcact tgcaccatgg ccgaaactcc
     3781 cggtattccc gatcatagca aatacgccat aatctaattt aatgaggcgt tgtgcttcat
     3841 ccggcgctag gcccccatag cttttggatg caagtaattt accgcttcgg tctaagagca
     3901 gtgttacaac gtctttatta ttaccggtta tttggattgt tcctacagcg agaaagttac
     3961 cgtctgatgt ttctgtgata tccgtgccgt agtccgcttt ttcggatcca atggctttat
     4021 accaaaccgg attgaggtag gcgtcagtaa gaactatcaa tatgtctgaa ttgcctctat
     4081 agttatcggt acgccctatt ataataaagg aaccgtccga attttgatga acagacttta
     4141 ataaatcatt gcccttgaac cccaatcgcg tgtaacttaa tgaagtattt ttggaggata
     4201 atgactcgca cgagttagcg aggacaatca aaagaaaaat agaaaaaaat gatatgattt
     4261 tatcaaacac gatacacgtc caaaattata tttcataaat cgtatagaga attggtatcc
     4321 ttcgcggata ttcgtatttt aattatacag aataggaata acaatataaa ataggtacaa
     4381 tgaaaagatc acaaaaaaaa ctgctcttct taggaacatt cttatgtgta gtgatattat
     4441 taattcctga aagcgaattg aaaattgata ctacttcgtt tgatcactca tttgaatgga
     4501 atcaggatag cttgtggcaa acgctggagc gtgaatttgt ggcatcacgt tatgaagagg
     4561 gtttacaaac tgtcaaattg atacaagtat ccatggctca actgcaaaga atgacggaca
     4621 cgctgaatac gactacagtg aacacaagtg acgagttact ttggcgtatt gaaagaagtg
     4681 tttattattt agctactcgt gttgcggtaa agccggaatt cgttgctgat ttttcgcgta
     4741 tcattatgaa tttacgattt gtggttaaga aacaaagccg taactgggat atgaaagatc
     4801 gtataaccag agaaagactt tatcggattt tgtacggaat gcgtacggca cttgaagaag
     4861 ttctattaca atcgcagacc gataaatttg ccgagattaa tcctggacat gctgagtctt
     4921 cggttacacc acatgcagat gttttaggtg tcatgttaca tagcggtgat attttggtgt
     4981 cgcgcggcgg tgcgccgaca tcggcattga ttgcccgagg taatgatttt cccggtaatt
     5041 tttctcatgt ggcgttagtt catattgatt cggtgaccgg aaaaatttcc cttatcgagg
     5101 cgcacattga aaaaggtgtt gcgatcgcta catggaatga atatatcgcc gataaaaaat
     5161 tacgcatttt gatactgcgc ccacgccatg atattcctgt tttagcgagc gatccgatgg
     5221 caccgcataa agcggcggat tttgcccttc agttggcctt gcaaaagcac attccgtacg
     5281 attttgttat gaactatcgg gatcatggtg cattgttttg ttccgaagtg gcttcgtcag
     5341 cgtatgaatc ggtaggaata agtttgtgga tgggtatttc gcgcatcact tcgccgggta
     5401 ctaagcaatg gttgtcttat tttggcgttc ggcattttga aacgcaggaa cccgcggatc
     5461 tcgaatacga tccgcaattg caagtcgtag ccgaatggcg aaatgcatct gcgttgttac
     5521 aggatcaact ggataatgcc gtgatcgata tcatgctgga agatgccgat gccggcaaac
     5581 cgatagagta taacatgtgg tatttaccag cggcacgatt ggctaaaacg tattctgtgc
     5641 ttttgaattt ttttggaaaa gtggggccaa tcccggaggg aatgagtgcg acggcggctc
     5701 tgcgaaatca atggttttct gagcgacatc atgctattcg tgccatactt gcggaagaag
     5761 tgaagaaatt tagagagaag aaacaatatt ttcccccatt atgggaacgc caccgactgg
     5821 cacgcgaagc aaaaaacaag cttgatatga attaacccga taatattgcg tgtaggaacg
     5881 aggtataaaa tttttaatta aagatttaag actcttttga ctttaattaa aattatgtat
     5941 atattgtgtt gcaggttcaa aacgtgaacc actatgataa aattcaggag aagttttatg
     6001 aaacgtttaa tgatgttatt agtgattacg ggtatcgtat tgagcggatg ccaatctcaa
     6061 tcaaagcctt cagataattc ggacaagggt agcgccaata tgccggccgg tatgtcggca
     6121 gtgaatgaca atgtatccca gaagaacatt gttcaaattg ccctcggatc taaagatcac
     6181 acgacgcttg tcgaagcact taaagccagc ggccttgtgg acgtattggc caatcccggt
     6241 ccgtttacgg tttttgcccc gacgaatgca gcatttgaaa aattacccaa aggtacagtc
     6301 gaagaacttt tgaaacccga gaaaaaaagc gcacttaaaa acgtattatt ttatcacgtg
     6361 aatgtgagca tcttcaaagc cgaaagtttt cgtgacggtc aggtgatgaa tatgtttagc
     6421 ggccagccgg ctacatttag cgtcaaagac ggcaaagtca ccatcaacgg aaatgccacg
     6481 atcatcgcat ccatccctgc atctaatggg attatccacg tggtagacgg cgttatgctc
     6541 gctccggcaa aataattatt agctgtattt aaccgttttg tgaaacgtag agtatttgca
     6601 aaacggtttt ttattgttta atgctaaatt aaataactat atagtttttt aatttcaaaa
     6661 cagagctttt aaatcgtttg ttgacaaata tcattggatg tggaggatat ttgcctttct
     6721 ttcatttgaa agaaattagg aaagggcgtt gctatgagtt ttgatcccat gcatgcgata
     6781 caagatcact gggtcttcgg cgaatacggc gatgtcaatc cgtccattac tgattcttcg
     6841 acgtatacat ttatgtcgcc ggataaaatg cgtgaattat ttgaccacga aatcgaaggt
     6901 tgttttttat attcgcgcca ctggaacccg atcaataaag aactttcacg ttcacttgca
     6961 gcgctcgaag ccagcgcatc cgctcaggta acggcgtccg gcatggccgc catcagttcg
     7021 agcatactcc aatcgtgcat gaccggcgat gaagtcatat gcagccgtac ggtctatggc
     7081 gggacgtacg cgctattcaa aaattttctt ccacgactcg ggatcaaagt acattttgcc
     7141 aatattcaag atattcctgc cattcaaaaa ttaatctcgt ccaagacacg ggtgatctat
     7201 accgaagcag tgagcaatcc tttactggaa gtagctgata ttccatcatt ggccaaactt
     7261 gcgcatgatc gcagtgccaa attagtcgtg gataacacct ttagtccgct cacgatttct
     7321 ccgattcgtc atggtgctga tgtagtggtt catagtttga ccaaattcat caacggaacc
     7381 agtgactgtg ttgcgggatg tatctgtgcc agtgacgaat ttattcatca actcacggat
     7441 atcaattccg gtgcgtgcat gcttatgggg cctgtactcg acagtatgcg cgccgcaagc
     7501 attctcaaaa atctgcatag cctccatatt cgtatccaac agcacagccg caacgcgcta
     7561 tatattgcat ccgaacttga aaaattgggc ttacgtgtct attatccggg attggtgtcc
     7621 catcgccaac acacacttct gaagtctatg atgaatgcac agtttggatt tggcggaatg
     7681 gtggcgattg atgcagggac atcggaaaaa gccgaggcgc tgatgacgcg gatgcaacaa
     7741 gaaaaagtcg gttaccttgc cgtgagtcta ggatacttca aaacactatt cagtgcaccc
     7801 gggcatagta cgtcttcgga aattcctgaa gaagagcgtc aggcgatggg tcttggtgat
     7861 gggttggtgc gtatgtccgt cggattagac agcgatatag ggcgttcgtt cgcgcgaatc
     7921 aaaacatgtt tggcggaatt aaaattgata taaaatttta aatcataacc aatatttact
     7981 cttaaccttg taaggagatc gtatgaataa aaaaatgatc atcacgattg cggccatagc
     8041 tgttatcgct gtcggtgctg tctatgcgta tcgtatgaat acatccgaaa cttgtcctcc
     8101 gggtatgacg ggcggagatt gctgcaaatc aacagaagta aaagcgcagg aagtaaaatc
     8161 ttctgaacaa caagcaaccg cttcaacgga aacacatagc tgttgcccga gcggagcgga
     8221 aaagaaacag taagaatatt taaaaatgat tttaaggaag agtttttggc agaagtaaat
     8281 gctgattttt tcagtataaa attttcaaca taatcgaata cttgaataat acatatgccc
     8341 ggcttagtcc gggcatatgt attttgttgg gtaacttaca ggcacgaatt taatttttat
     8401 attttaggaa cggttttacg ataaaggttg gttggatttc caaaatgaat gattgtattt
     8461 ccaagtatga ttgtttgggt aaagcagtgt atagaactgg gagaaatgca gaattgaaag
     8521 taaccaattt gcaactacag gattccgaca ggatcgaact tagtattctc taagtcaaaa
     8581 tatgtatgga aatcgcaaaa aagaagcgtg ataagtgtta ttacttactc atttgaatgt
     8641 aggtataaga aaaaacttgc tatgtttgaa aaaaaaagta acttattgag ttaatattct
     8701 tttctggaat ccgattcgtc acattcactt tcctgtgagt taagaagcag tgtgactcct
     8761 atcttgttct cgtgtgattt tataatttgt ttgaccgctt taggatttaa agcaacatat
     8821 ttcagccgag ttcccttttc atgatttagc ggaataggat aaaattgtag gctgtcgcaa
     8881 atatgattct tcattattaa tattcttttc taactaatct ctaacaaaaa acaggaggag
     8941 tgaacatgcg ctatgggtta caaaagcctt tcttttggct tctatcagcg tttgtaatag
     9001 ccggaatact gatcgttggt tcgagtgaac agaggtcaac ggctaatatg aaaaaaaagc
     9061 accttaaaaa cacacctgaa gccctaaagc aagcgcttct agaacaaaaa attaaacgtc
     9121 gtgccgaagg ttacgctaaa ccggatcaac ctgaaaaatt tgacattttg catcgtgaaa
     9181 ttcgtaccca gtatggtaag caactacctg attaccaaat gggctataaa aatatcgaaa
     9241 tggagaaagc cggaattata aggcgtgaag gtgagaagta tgtacgcaat caaatgatgt
     9301 catcttcaac attaagttgg acttcacgtg gcccgtcaaa ttttttagga agatcgcgtg
     9361 gccttgttgt tgatcctgat gatgcgaatc atcttactta ctaccttgga tccgttggcg
     9421 gtgggatttg gaaaacaaca aatggaggct catactggga aaatttaact cccgaatttc
     9481 ctaatctttc gacgacagtg attgctatgg cctcttcaaa ccacaatatc atgtatgcgg
     9541 gaacagggga agggtatcaa gccgcagttg gcatgataaa aggcgatggt atttttagat
     9601 caacagatcg tggcgccagt tgggcacaac ttagcgcgac ttcatctaat agtgattttg
     9661 catacgtcaa tagattaatc attgatccca ataattctga tattgtagtg gccggtacaa
     9721 attcagcaat tatgagaaca acagatggtg gtacaaattg gaccaaagta catactgccg
     9781 gcaataatcg gatacaacag gtattagctg atccatcaga ttttaatatc caatatgcaa
     9841 cggcagaagc gttggggttt ttgaaatcga cggatgcagg tatcacttgg gcacctagca
     9901 gcacaggtat aggtgctgtt gatcgtttgg aaatggctat tgcgccaagt aatacaaatt
     9961 tattatatgc tagcgttgag agcggtacca actcagtttt gtatcgttca accaatggtg
    10021 gggccaactg ggctactgtc gctgatacaa cggggcaaag cgcttccagt atagcatggc
    10081 tcggtgcaca aggatggtat gataatacca tagctgttca tccctatgat ccaaacattt
    10141 tgtttgtagg ggggatcaat atttatcgta taaatatttc caacagcatc tcagctgggc
    10201 gtcgcggaac aacaagaatg actaattggt atccgggttt gagtcgtcct agcggaggta
    10261 cctatcccta tatacatgcg gatcagcata atattgttat gattccaata agtgagccta
    10321 ataatttttg gattttacat accaatgacg ggggttttgc ccgttcaacc gatggcggtc
    10381 aaacttggtt gtttgatgaa gacgaagcta aaggaatcgt gacagcgcaa ttctatggtg
    10441 tagataaaat gcccggtgcc agtgcatata tcggtggaac acaggataat ggtacttgga
    10501 tgtctcctgt agatgcgaca gttgactcat cttggtatgc gatgctaggc ggtgacggat
    10561 acgatggtgt atggaaagcg aatgatccaa atcagattat cgggtgtttg tatgataatc
    10621 gtctatacaa aacaaccgac ggaggacaaa cctggaattc agcaacaagc gggcttttag
    10681 atgttgataa tgctaatagc gcatttgtta cagaaatcgg gcgttcaaaa cttgatccgg
    10741 acctactgat agtacctggt ggttctggtt tttggcgttc cgaagatttt ggtgatagtt
    10801 ggacgttagc gcccattaca agcagttatg gcttttccac ggcaagaaat ttggctgaaa
    10861 tatcattagc cgatcccaat attgtttggg ccggaactaa catgagtgcg gctggtaaac
    10921 tccacgtttc caccaatgct ggtaaatcct atacagcaac caacaattat acggctgtca
    10981 cgttaggacg agtaacaggt attgcgacgc atccttataa cccaaatgaa gcttttgcat
    11041 tattttctat tgcagataaa ccaaaagttt tgcgaacaat ggatcgtggt caaacatgga
    11101 cagatataag cggtttcggc gctaacagca gtagcaacaa tggttttcct gatgtggcga
    11161 cttactcttt attggttatg ccgaccgata ccaatgaaat atgggcagga acggaaatcg
    11221 gtatttttcg ttcaacggat aatggcgcga catgggccta tgccaataac gggttgccgg
    11281 ccgtctccgt atgggaacta aaaattatcg ataatgaaat agttgccgca actcacgggc
    11341 gtggtatttg gacagctccc ataagcgctc caccgcttcc gactgttgtg ctcgggccaa
    11401 cactaaaaca agcctatcag aaccccgcaa ccggtttagt taatgttgag ctaggtttgc
    11461 gaaacacata tgactcgacc attgttttta tgggtaatac ccgggctctt acgttggggt
    11521 caacaggcat aaatgacagt acagtatctt tttcatcaac gcaaatcggt tcggtaagca
    11581 taaaagcttc ttcatacagg aatggcacat tgtatgaaac ggttgcaaaa tcggttacct
    11641 tggtgcaagc cgtggctcgc aatgattatt acaatgcttt taatacgtcc acatcagcca
    11701 atggagagtt tacgggtagt cttttttcag ctacgaagcc aagcggtttt agctcttttg
    11761 cattgcatac tgcacacccg tatacaaaca atacgaataa tacttttcaa ttaaattatc
    11821 cgataacggt tgcaagcgat tcatcaatga ttgtttttga tgaaatcgct attgtagagc
    11881 cgggagaccc tggagtaccg tttcctgaac ccgaattctg ggattatgta gttgtagagg
    11941 ccacttcaga tggtattagt tgggagcctg ttattgatgg atgggacagt agagctaact
    12001 cgacgtggtt aagcacattt aacagcgtgg gttcgggatc taattctatg tttaaacaac
    12061 gtcgaattga tttgacagat aagtacgagc caggtacaaa aatttttata cgatttagaa
    12121 tgtttactga tgctgcatct acaggttggg gatggtgtat agataatcta aaaataaatg
    12181 caatagaaac tcccccgacg gttaccgttg gcgctttagc atcgcctgtc attaatgtag
    12241 ctaagtttgc tgtcagttcg aatcgtggaa tgtcaactgc tgcattgact gcgggcgggc
    12301 aatctgttgc gctcagtcgt ttgggcggtt ccttattctt cggatcatat tcaattacgt
    12361 ccggtacagt atcaataact gcgacatgct ctgattctgt aggcaataca agcacaacat
    12421 taaataagca gtttaccgta acacccttgt caaaatcaag ctccttaggc ggttattccg
    12481 tcacaggtag cggtgaagga tatgttgtgg catccttagt ccaggcaacc gatattccat
    12541 caggttggat tgaaataggt tccatgtatg aggctggcat taccggtacg ccgactaacc
    12601 tcaaaataac atacgatctg ccaaatgaaa gcactttgga tcgaatgata cctggatctt
    12661 cgcaacgata taaaatcggc ctctatgaaa aagacggcaa tcaatggaag cttataagtg
    12721 cggagtccaa cggaaaatta agcggtgagt ttagccataa agccatcgcc gcattttaca
    12781 atccggattt tgagggtatt cctacggagt ttgcattatc acagaattat ccgaatccat
    12841 ttaatccgcg gactacgatt cgatatgatg tggccaaggc gggtaaagtt gtgatcaagg
    12901 tgtataacat gctgggtcag gaagtagcta ctttgatcaa tgcgcaacgc gatccgggaa
    12961 ggtattctat ccaatgggat ggccgtaata atgcaggttc ggttgtggcc tcgggtgttt
    13021 atttgtaccg gatgcaagtc ggtgatgtgg ttcgcagtaa gcggatgctg tttttgaagt
    13081 aactcaaaaa aatgataagg agtcgagata tatgaaagca aaaaataagc tttggatgat
    13141 atgcttagtt tccatagcaa tttcatcgtg tggcggtggt ggaggtaaag acaagatcac
    13201 tttgaccaaa gcgaccgatc aatttgcaac cgggaactat acgaccgcat tgcgtaattt
    13261 tgagaatttg attgcatcgg agggcgctcc ggcggtcgcg ggtgcgggtt ggtgcttggt
    13321 tcgattgagc aatgtagccc gcgccgatag tttttttgtg acggtagcag cggatagtaa
    13381 tacggatgcc tatgcgggtt ggagtttttc gtcgtgggca ttaaacgatc cgagtgaagc
    13441 tatttcacgt gctgattttg ttatacgtaa ggatgcgaat tttcaaatga gtttggatac
    13501 tcgcgttacc aagagtcatt tagtttggat ccaagcgagc agttatttgc agttgggtga
    13561 ttatacgtct tgttttaaca agatcaagct attggatgcg gcatatacct atccgagcgg
    13621 gacatcggtt gaaatagcaa ccgcactgat gcaaaagttg caatcgcttg gttcagcttc
    13681 ataaacttct ttccaatcac tataaaaaaa gcaaggttta aagccttgct ttttttattg
    13741 tttttttttg acggttacaa tatcatagca ggtttaatct ctacgcaatt ttttcattaa
    13801 ctaaaaaagg gaaaaatcat gaatcttaga acttcatggc atcgctttgt aacgatcgta
    13861 attaatttta gtagtctttt tatcatcctc acttctatgc tgcaagggca aaacaatcca
    13921 aatccgtatt acggtacgga tgggtatgtt aatgatgtaa aagccagtcc ggacggaaat
    13981 acaatttaca taggaggaga atttaattcg gttgtttcgg ttaccggttc gatgatgttt
    14041 tttgatgcga ataaccagga aaaaacagat tttcctcgcg ttttaggcac aatatatgat
    14101 gttgcttatc atccccaata tgggtggttt attgctggaa attttactca catcaacggc
    14161 gtaaaacgta agtatattgc aagaataaaa acagatctta ctttagatat gaattggaac
    14221 ccgatcgttg acggcgaagt gcgaagcatc gcggtaaatg aagattttgt ttatttcgga
    14281 ggcaattttt ctaaagtatc tgatcagacg agatatcata tcgcggctgt aggcgtagcg
    14341 gatggcgtac tttcagattg gaatcccggt gctgatggga tcgtatatga catgattgtg
    14401 tcttctaata cgcttattat tggcggtcaa tttactgaaa ttgcaggaga agaacggttt
    14461 agcttagggt ctatagatat ttctagcggt aatgctaccg cttgggcgcc acatgttact
    14521 tacagtgaaa ataacccggg tattgtatat gcgattgtag tcagctctaa taatgtttat
    14581 attgggggag aattttttta tctaggtgga gtagaaagac agcattgtgg tgcagttgat
    14641 atggcatccg gagcagtaaa tccttttaac ccttggccaa acactatagt cttttcgctc
    14701 tacgttaaaa attcgactct ctatattgga gggatgttta cgcagatttt ttctcaagtt
    14761 agaaatcggt tggcggctta tagcttatcg ctaaatcaac tcaattcatg ggatcccaat
    14821 attgactcgt acaatacatt tccaaatgcg aacagtagtg ttcgcgctat ttctgtttta
    14881 ggcgacaaaa tattggtagg tggaattttt acaaaagtag gttcaacctc aaaagcgaat
    14941 tatgcgcttt tcgatttgaa tacaggcaat ttatcttcta taactaagac tccttccggt
    15001 tcagtattta ctattactac ggcctcctct gacaatgaag cgatagcggg cggttccttt
    15061 gatagcttta gccagtatat aacacgttat cgtttagcag cttttgatgc gcagactggt
    15121 gcattgcgcg attgggctcc ggtagtagat ggagcagtga cgagtattga agtttttgaa
    15181 aacacagttc ttataggtgg ttattttaat aaggttaata atatctctcg accgtatgcg
    15241 gcctctctta ttggttatgg agccaccggt tcagggcagc taaataactg ggcgccggga
    15301 ctgaatggga ttgtttcgca gctactcata gccggcgaat cgttatatat tcgtggcagt
    15361 tttacttcag ttagtgaaaa taatcgttta aacattgctt tgtttgattt gaatacaatg
    15421 caattgcggc aaacttcgct tcaaattccg tatccaacgt cttatttaac gctgagggca
    15481 atgggcgcag tagattctgt tctgatgttg ggcattcaat atttcgaggg taattcattt
    15541 aaaagcgtgc tatctgaatt taatacccgt acggggcaag agatcagaaa ttcaccgaca
    15601 aataaaacca taaatgctat aactgcaact tcatcggtga tttatttggg tggttatttt
    15661 acgcaggtta agggtgttta taggtcgggt gcagctgctc ttgatcgtaa gaccggggag
    15721 attaaagctt gggatgcaaa attgagcggt ggtttaggcc taacaaaaat ggttactgcg
    15781 ggaaattata tatatatgac cggtgatttt gatagtgttg gggcaattcc tcaatcaggt
    15841 tttaccaaaa ttgatatgat ttccggcgta tgttctccga tgggacatac atacaacggc
    15901 agtatagccg ggatggatat caatgagcaa aatatatata tcggcggttc ttttagtcat
    15961 ttcaattttt tatcaaataa tgattttacg gtcattcaag attcgacaat tcttgttccg
    16021 ccgaaaaatc cgatgtgcag cccgtcgctt gaattggatc gtgtgattga tctttcttgg
    16081 accaaatctt caggagcgac ctattataag atatatagag ggctttcatc tttttttgac
    16141 acgacaggta actgtatagc tactgttggc gatgtgaata cttatcggga cactttaggc
    16201 ggatatataa aatactatta taaaatcggc gcatttagtg aaggtaattt ttctaaattg
    16261 agcgcaatag cctctgcgac agcccctgat tttatagcgc cagaagccaa tatcgggtta
    16321 cttgtgccgg gatttggcac acatgtcaaa atcgttgtta cttctaatga gagtctcaaa
    16381 tcaatgattg ttaatataaa taatagttcg gttacgatga gtcagtcggg tagccttttt
    16441 tttggcggat atacgatcca acaagcggga cagctaacta tcacagcgac cgccaaggat
    16501 caatctaaca atacgacatc gcttgaaaaa tcgtttaacg tgcagacgat cggccgcccg
    16561 ttttataatg atttatataa aataacagta tcaggatcag atggttattt tttgacgact
    16621 gatgatgaca aatctttcag cggttcggat gatttgatac cgctccaaaa accaaaaaca
    16681 tttcttacaa ccagtattcc aagtcaaatc gctttggcat tcactgaata tgaaataacg
    16741 gcgctttcag gattaaaaaa acgtcaaaac tttactaatt cggctatcgg attgtatgcc
    16801 cagatcggcg atgagcgaat tttgcttgca aaaggtgggg attatacaaa ttcggtggta
    16861 ttagaaaaat cagtgcagga taaattaatt tcattgctat ctacgtgtgg attgactatc
    16921 gccgcatttt acaatccgga ttttgagggt attcctacgg agtttgcatt atcacagaat
    16981 tatccgaatc catttaatcc gcggactacg attcgatatg atgtggccaa ggcgggtaaa
    17041 gttgtgatca aggtgtataa catgctgggt caggaagtag ctactttgat caatgcgcaa
    17101 cgcgatccgg gaaggtattc tatccaatgg gatggccgta ataatgcagg ttcggttgtg
    17161 gcctcgggtg tttatttgta ccggatgcaa gtcggtgatg tggttcgcag taagcggatg
    17221 ctgtttttga agtaactcaa aaaaatgata aggagtcgag atatatgaaa gcaaaaaata
    17281 agctttggat gatatgctta gtttccatag caatttcatc gtgtggcggt ggtggaggta
    17341 aagacaagat cactttgacc aaagcgaccg atcaatttgc aaccgggaac tatacgaccg
    17401 cattgcgtaa ttttgagaat ttgattgcat cggagggcgc tccggcggtc gcgggtgcgg
    17461 gttggtgctt ggttcgattg agcaatgtag cccgcgccga tagttttttt gtgacggtag
    17521 cagcggatag taatacggat gcctatgcgg gttggagttt ttcgtcgtgg gcattaaacg
    17581 atccgagtga agctatttca cgtgctgatt ttgttatacg taaggatgcg aattttcaaa
    17641 tgagtttgga tactcgcgtt accaagagtc atttagtttg gatccaagcg agcagttatt
    17701 tgcagttggg tgattatacg tcttgtttta acaagatcaa gctattggat gcggcatata
    17761 cctatccgag cgggacatcg gttgaaatag caaccgcact gatgcaaaag ttgcaatcgc
    17821 ttggttcagc ttcataaact tctttccaat cactataaaa aaagcaaggt ttaaaacctt
    17881 gcttttttta tgaccgtttc tgattttttt tgcatattat tggtacatct tagccgtgca
    17941 attgatcttt tgaaagtatg cgtctttttt tatccttgtt agttctacta tttgccattt
    18001 ctattaccgc tcaggacatt cctccgcgta ttaccattta taacccgccc ggtgcgcatc
    18061 cccaaagttt tgaattaacg caagacgcgt ccgggatcat ttatgcggtg aattacaatg
    18121 gcatatttgg atttgacgga gtgcgttgga aaaaattagc agggcgattg gaaacgtacg
    18181 attggaacgg tataacggcg gacaaaaaaa cagggcgcat ttatatagg
//
LOCUS       DAOQNQ010000037        17845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1109_length_17845_cov_6.460483, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000037 DAOQNQ010000000
VERSION     DAOQNQ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17845
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1109_length_17845_cov_6.460483"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PK754_04160"
     CDS             complement(<1..374)
                     /locus_tag="PK754_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB08729.1"
                     /translation="MTKYVWRSLLFFEIALISLICSVTAQVRFVDLDRLSLEEGLSQS
                     SAICMLQDRQGFLWIGTEDGLNRYDGYSIKIFKADPADSGALQNAWITSLFEDHTGNL
                     WVGTRGGLHRYDAAKNRFISIPL"
     gene            643..1266
                     /locus_tag="PK754_04165"
     CDS             643..1266
                     /locus_tag="PK754_04165"
                     /inference="COORDINATES: protein motif:HMM:NF025867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNB08730.1"
                     /translation="MDQNIKTEQDSAENKIIKAARSIFHRRGFDGARMQEIADEAGIN
                     KALLHYYYRSKDRLFQAVFEEAISRFFPLVLHMINSEAPLEQKIEKLIHTYIDFIKNN
                     RFIPGFVLHEMTTNPERMRRIFEERGIVPKLDVIKRQIREGIEDGRYIPIEPEQLLLS
                     ILSQCVFPFVAKPMVEFFFSMDEKQFNEFIERRKAQVTMLVLNGLRV"
     gene            1276..2157
                     /locus_tag="PK754_04170"
     CDS             1276..2157
                     /locus_tag="PK754_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNB08731.1"
                     /translation="MKTFITITLFLITLTACSKKNNTADAYGNFEAVETLIAAEAGGK
                     LLFFDVEEGVTLSEQQRVGCIDTTQLALKRIQLVASKQSVDARSANVLAQMEVLQEQR
                     RVAMTEKTRIENLYREKVATQKQMDDITGQVRVIEKQIASTEAQNAGVLSELAGLDAQ
                     IRQINDQIQKSLIINPISGTVLAKYVQSYEIVSFGKPLYKIADIRVMHLRAYISGDQL
                     SHVKIGQKVRVAIDDNATDNRSLEGEVIWIASKAEFTPKIIQTKEERVNMVYAIKVKV
                     QNDGSLKIGMPGEMYFQ"
     gene            2188..2586
                     /locus_tag="PK754_04175"
     CDS             2188..2586
                     /locus_tag="PK754_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08732.1"
                     /translation="MKPLIIKLLISLSATIFGLATITAGGAAIFFNEGVFGTVIPWVV
                     WFNFFSGFIYVAASLYVWIDPRVAYKLSIYIFITTSVIFVLLGIHIASGLAYEKRTVA
                     AMGFRWIVWGIISLIGYYVFVKNQADNTKL"
     gene            2583..3503
                     /locus_tag="PK754_04180"
     CDS             2583..3503
                     /locus_tag="PK754_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB08733.1"
                     /translation="MISVEADHLTKRFGKTSAITNLSFQVKAGELFGFIGPDGAGKST
                     LFRILTTLMIPDEGRATVSGLDVVRDFRELRKRLGYMPGRFSLYQDLSVEENLQFFAS
                     VFGTSIQENYDLIRDIYVQIEPFKKRRAGQLSGGMKQKLALSCALIHKPEVLFLDEPT
                     TGVDAVSRQEFWHMLKRLQTKGITIIVSTPYMDEAGLCDRIALIQSGKILDIDTPADV
                     VGKFRSPLYAIKSRKTYLLMEHLRGSAETQSIFPFGEFLHYTDRRPGVSEQQLRDELR
                     TEGFDDVEVMRTEPTIEDSFMALMEQHETQ"
     gene            3490..4245
                     /locus_tag="PK754_04185"
     CDS             3490..4245
                     /locus_tag="PK754_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB08734.1"
                     /translation="MKPNKSDAAITVRDLTKRFGSFIAVNAITFDVGRGEIFGFLGAN
                     GAGKTTAMRMIIGLLAPTSGTAQVAGYDVYTQTESIKKNIGYMSQRFSLYEDMTVQEN
                     ITFYGGIYGLSDAAIRTKSEMLLERLRMHDVARTMLRELPLGWKQKLAFSVALLHDPA
                     IVFLDEPTGGVDPITRRQFWDMIYEAAHRGVTVFVTTHYMDEAEYCDRVSIMVDGRMA
                     ALDTPRGLKQTFEAQSMNDVFIRLARPQQSKEL"
     gene            4242..5342
                     /locus_tag="PK754_04190"
     CDS             4242..5342
                     /locus_tag="PK754_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB08735.1"
                     /translation="MKSFRAFIVKEFFHILRDKRTVLILFGMPVIQMILFGFAIRNEI
                     NDARIAILDHAHDARSQEVTQKILSSGYFQNVQTLTHDDQIAPAFETGDVKMVVVFEP
                     QFTARLYHEGVAHIQLITDATEPNMATTLTAYATSIIRDYSANLNPHAPTPIVTEVKM
                     LFNPEMRSVYLFVPGLIALLLMLISALMTSITITREKELGTMEILLVSPLRPIQIIVG
                     KVLPYLLLSLVNTSTVLLLARFVFEVPFRGSYALFYAEALLFIMTALSLGLLISTIAR
                     TQQVAMMASLAGLMLPVIILSGFIFPVSSMPVPLQIFSNIVPAKWFLIIVKGIMLKGV
                     GIEYLWKETLVLAGMTLLLMTISARKFKIRLE"
     gene            5345..6457
                     /locus_tag="PK754_04195"
     CDS             5345..6457
                     /locus_tag="PK754_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB08736.1"
                     /translation="MRTIRFILRKEFLQIFRNKGMLPIIFVMPFIQLLILSNAATFDV
                     KNVKFQIVDRDRSDLSRQLIERFTSTHHFILTDMPTHYAQAEESVLKNEASLIVEIPV
                     FFEKDVITGATVKLQLVINSEDGSTAGVIQSYAAQIIQDFTRQVVPFQQTQGMTQRIE
                     IIARNWYNPELNYKHYMIPGILVALVTMIGLFLSGMNVVREKEIGTIEQINVTPIKKH
                     QFIIGKLLPFLIIGLFELAMGLAIGRLVFDLPMLGGLPLVFGITIIYLILVLGLGLFI
                     STVTETQQQAMFIAWFFMVIFMLMGGLFTPLDSMPLWAQQTMWINPVALFIRILRQVL
                     LKGAPLHDIIPYVSALIVLAVTMFTLAVIRYRKTSE"
     gene            6536..7150
                     /locus_tag="PK754_04200"
     CDS             6536..7150
                     /locus_tag="PK754_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08737.1"
                     /translation="MKYIVIFFVFVMTIVSCKPGPKPAPENAPAGNPHAGNPHANIDM
                     NAPGHVVFKAPEVWKSETPTNSMRKEQYRLPGSKPEDDAELVLSYFPGAGGSVDANLS
                     RWYGQFKQPDGGDTQSKAVIKNTENNKLKITVVRVSGNYMKPRVAGQMGGPVDETPAY
                     AMWAAIVEAPNGPWFLKAVGPQTTMDKYSKEFEQLIGTIDYILD"
     gene            7335..8324
                     /gene="thiL"
                     /locus_tag="PK754_04205"
     CDS             7335..8324
                     /gene="thiL"
                     /locus_tag="PK754_04205"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNB08738.1"
                     /translation="MKSKKTGDEFSLIRQLHAILESGYPTGKNVLCGIGDDAAWVRSS
                     AKQQVITTDAMVEGIHFDRRYMAFEDIGYKSISVNVSDIAAMGADPSFAVIALQMDPQ
                     TGTKNIHALYRGITKACRNYNVQIVGGNISRSSAFSITVTLIGNVAERNRPWLRSDAR
                     IGDVIAVTGDLGGSAAGLMFYDRLKKKPAYAWQRHCRPDARLEWAQILRRKNIRVHAA
                     IDISDGLSSDLAHVCRASRCGALIEAACIPVHAEIKKLSDVLKIDSMMWALHGGEDYE
                     LLLTMSPKEFVKAKKILKNNIHAIGTIVAGTSVVIADASGNRKTLEPAGFRHF"
     gene            8335..9549
                     /locus_tag="PK754_04210"
     CDS             8335..9549
                     /locus_tag="PK754_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNB08739.1"
                     /translation="MAQSTGNINERIERIQKDIARNGYDGWLLYSFRKNNPVASSILS
                     IPEHLTQKRRFFYYIPAQGTPKKLVHGIERYVLDTLPGEKVIFSSWQDLEAGLKAITA
                     GHKKIAMEYSPECNIPYVAIVDAGTIELVRKTTQAEIVSSADLVQYFDSTWDDQQLQL
                     HVEAGKALTDIIPQVWGFIREKITGRQKVTEYDVQQFIHNQFIERGLTTGDLPNCSVN
                     ENAGDPHYEPTKDHCKEIHEGDLVLIDWWAKKNHPRGVYADYTQMGFVGKTVPEKFAK
                     VFDVVVGARDAAIAFIKEEIAADRPLPGWKIDDVCRAHIVQHGYGEYFIHRTGHSIGE
                     EVHGNGANIDNLETKDNRRIIPRTCFSIEPGIYFVGDFGVRTEVNVYVGDKKDVLVTG
                     TPMQSSIFPILA"
     gene            9571..10041
                     /gene="greA"
                     /locus_tag="PK754_04215"
     CDS             9571..10041
                     /gene="greA"
                     /locus_tag="PK754_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010516379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNB08740.1"
                     /translation="MSKFYVTHEGLLKLKEEIQHLKNVKRPEIANRVQVAREHGDLKE
                     NAEYHAAKEELQLLQSKINEMEMKVMNAVIVKEDEIPTDKVYILATVDLQNVKTKEKL
                     TYTLVPDNEADWENNKLSVNTPIAKGLLGKKVGDVAKVKIPAGELELKIIKISR"
     gene            10045..10278
                     /locus_tag="PK754_04220"
     CDS             10045..10278
                     /locus_tag="PK754_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08741.1"
                     /translation="MNEDTSQPDIQDEETSGEAASDTRRKRMWQIFGWSVIGALLLLI
                     VLNLFQWASPEINQYGLPVIVVLYVVYVFFRKR"
     gene            10288..11445
                     /gene="bshA"
                     /locus_tag="PK754_04225"
     CDS             10288..11445
                     /gene="bshA"
                     /locus_tag="PK754_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HNB08742.1"
                     /translation="MKIGVTCYPTYGGSGVVATELGIELAKRGHDIHFITSDIPFRLN
                     TFRDRIYFHAVEVMNYPVFDHSPYSLSLAARMAEVAEFEDLDLLHVHYAIPHAPSAYL
                     AKQIIRKNNRIKDLKTVTTLHGTDITLLGMDRSFLNLIKFSIDESDGTTSVSKYLRNR
                     TYGEFKPNRKIEVIPNFVDTEKYRPHDKPEDSSHGFLSRITPNHEKLIMHISNFRPVK
                     RVGDVVKIFAKVRENINAKLLLIGDGPERRMCEQLVQDFGLDEHVVFLGKQEAVAELI
                     CCAHVFLLPSEEESFGLAALEAMAAGVPCVTSNAGGLSEVNIHNETGYLEKIGDVDAM
                     AADVVKILTDQDVYQRFRANARQRVVDNFGTDRVVQQYIDYYQKVIEGKNT"
     gene            11442..12179
                     /locus_tag="PK754_04230"
     CDS             11442..12179
                     /locus_tag="PK754_04230"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNB08743.1"
                     /translation="MIAPKHIMIMAHRGVTYSGFPENSLPGFQAVVDEGYDGVELDVR
                     LTRDKELVVFHDIRLDRLTTTGRGMVRTKTLEELKKLTLRSDRVQTGIPTLKEVLTVF
                     QGKPVFVNIEIKSEMPLRGKIEERVIDLIYNMHMRKQVVISSFNPLVVKKCQRIDPDM
                     RMGFIYEKRLPNLNHRLAKGLVVDSWHPNSVGVNALLMERAWEARCAVYPWTVNDEKE
                     MQRMIDLGVDGLITDYPNIAKKILQRI"
     gene            12181..13320
                     /gene="carA"
                     /locus_tag="PK754_04235"
     CDS             12181..13320
                     /gene="carA"
                     /locus_tag="PK754_04235"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNB08744.1"
                     /translation="MNSNKSKALLVLEDGTFYEAWSFGADGEATGEIVFNTSMTGYQE
                     IVSDPSYYGQMIVMTYPLIGNYGISDEDFESRGPQAKALIVKEVCDYPSNWRSSHSLS
                     DLLKKNGIMGIEGLDTRAITRHIRNFGAMRAIISTTDTSVQRLLEKVKNAPRMEGANH
                     VDAVTCKTKYQWSEPNAEMWQFRENITGTHPYRIAVYDFGVKQNILRKFVERGCVVEV
                     FPSKTKADEILASNPDGIFLSNGPGDPEAVDYVIPEIKKFIGKKPVFGICLGQQLLGI
                     ALGAKTYKLKFGHRGANHPVKNLATGIIEITSQNHGFTVDPKTLNEKEIELTHFNLYD
                     GTLEGFRHRELPLFSVQYHPEASPGPHDSDYLFNQFMDRVKADKN"
     gene            13336..13680
                     /locus_tag="PK754_04240"
     CDS             13336..13680
                     /locus_tag="PK754_04240"
                     /inference="COORDINATES: protein motif:HMM:NF025062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CZB domain-containing protein"
                     /protein_id="HNB08745.1"
                     /translation="MEEIENAIAAHELWKERLMAAIETGHSVWAVDKVSVDDQCAFGK
                     WIHMIEADYKGDHFYTTVRELHAHFHKIAGIVLNLALSGFKKQATEIYQGEYQLASQH
                     LLFELHAWKKSL"
     gene            13732..14568
                     /locus_tag="PK754_04245"
     CDS             13732..14568
                     /locus_tag="PK754_04245"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNB08746.1"
                     /translation="MKIHRIAVMALAVLMSCQPAKKEPAKAEATTVAAKNITGEEISY
                     AADNITMKGFIAYDAAQTGKRPGVIVVHEWWGHNEYARQRAKMLAEMGYVALAVDMYG
                     DGKKAEHPQDAGKFASETMKNFKGAKARFEKAVETLKNNPNVDASHIAAIGYCFGGGV
                     VLNMARQGIDMKAVVSFHGSLGAAEPAKEGVVKSRVLVCNGAADKFVSEKDIEGFKKE
                     MDAAKVNYVFKSYDGAVHGFTNPAVDSLGKKFNLPLAYNAKADADSWADMTALFKEVF
                     GK"
     gene            14684..15829
                     /locus_tag="PK754_04250"
     CDS             14684..15829
                     /locus_tag="PK754_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNB08747.1"
                     /translation="MTKKLPLQKHLFDIPDEVTFLNHAFFAPQLRAMTEAGLQSVRAK
                     SSPWNYTPQHWFEDGETLRGLIAQIIGVASDDMAIIPSVSYGMAIAAKNITVRSDQNI
                     VVLHEEFPSNYYAWYYKAKDCGASLRVVHRTNQLNWTEAICENIDEKTAVVAVPNCHW
                     TDGARIDLERVSQKARSAEAALVIDASQSLGAFPIPMEKIRPDFLISVGYKWLLGPYS
                     LGYLYVDPKWQEGTPIEYSWLNRFGSENFSRLIEYTDKFRQGARRYDMGEFSNFINVP
                     MAIAGLRQIITWEVSAISESLLDLTSYAAQYAHSLGWVTISDSERIPHLLGIRHATSM
                     PSDIGQKLSENKIYVSIRGNAIRVAPYLYNQKSDMDRLFEVLKVYSR"
     gene            complement(16307..>17845)
                     /locus_tag="PK754_04255"
     CDS             complement(16307..>17845)
                     /locus_tag="PK754_04255"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNB08748.1"
                     /translation="YHIFFCTIIAASIGGVIALAQAPMKLSYGTANKEVIPVFTGDQE
                     YVSIKDVIALLDAGFFENTAKKKMVFTSNQHQFKVTGLNAFVVVDEKTTLQMPVTTQY
                     IDGAIFVPAVYFFSAINAYLSTPVVINGEKIIAKSAPAIKDTIKTSAVSISPNLKDVI
                     FEKKKNGLLMRIKTDGRFQSSDVEIWRNKNWLYVTVSGGYHSDLLAKDIKLSEQYKLI
                     KEALIFQHKNSTQFSFQLNSDIAGYDISVDDKKDEIYVAWRIHQSSDLEFDNGGSGTG
                     AIDKDQWKINKIVIDAGHGGKDHGASGKGGTKEKDITLNIALKVGKIIAERLKIPVEY
                     TRDTDTYITLKGRTQFANQKNAKLFVSIHCNSNKSRKAHGFEIFFLSPSRNAEALAVA
                     RKENEVIDMEDDKHHYGDFTDEKFILANIMQSVFVKESEELASYVSKGIDKQVDITNR
                     GVTQAPFYVLMGASMPSILVETAFISNPDEEKFLKSEAGQKKLSEGIVDGIKTFIDIY
                     ERSN"
BASE COUNT         4982 a         3704 c         4255 g         4904 t
ORIGIN      
        1 agcggtatag aaataaacct gttttttgcc gcatcgtagc ggtgcagccc gccgcgggtt
       61 cctacccata aattgccggt atgatcttca aaaagtgaag ttatccaagc attttgtaac
      121 gcaccggaat ctgccggatc ggctttgaaa atcttaatgg aatatccatc gtagcgatta
      181 agtccgtctt cggtgccaat ccaaagaaaa ccttgcctgt cttgtagcat gcagatagcg
      241 gagctttgag agagcccttc ttcgagggaa agcctgtcca gatcaacaaa acgtacctgt
      301 gccgttacac tgcaaatcag cgatattagg gctatttcaa aaaaaagcag gctccgccaa
      361 acgtattttg tcatgagaag ttttaaagtg gtttggatag gggtattata ataatttata
      421 tccctcattt tccataaaaa tttatgtttt atacttgtaa gaccgattca tcgaattttc
      481 taaattgttt tactaaacat aaacatcaaa gacagttttt tagcgtttgc tacataaaaa
      541 acttgacaca gctgtgttta taatatatat ttaactgttt agttaaacta tttaattaaa
      601 ctattaatta acaatttaag tttattatta tcaatattta aaatggatca aaacataaaa
      661 acagaacaag attcagcaga aaacaagatc atcaaagccg ctcgttccat atttcatcgt
      721 cgaggctttg acggcgcccg gatgcaggaa atagcagacg aagccggcat caataaagcg
      781 cttttgcatt attattaccg cagcaaagac cgtttgtttc aagctgtttt tgaagaggcg
      841 atctcacgtt tttttcctct cgttttacat atgatcaatt ccgaagcacc actggagcaa
      901 aaaatcgaaa aactgatcca cacctatatc gattttataa aaaacaatcg cttcattccc
      961 ggtttcgttt tacatgaaat gacgaccaat cccgagcgca tgcggcgcat atttgaggaa
     1021 cgcggtatag tacctaagct cgatgtcatc aaacgtcaaa tacgcgaagg catcgaagat
     1081 ggacgttata tcccgatcga gccggagcaa ttgcttctta gtattttaag ccaatgtgtt
     1141 tttccttttg ttgccaaacc gatggtcgag ttttttttca gtatggacga aaaacaattc
     1201 aatgaattta tagaacgccg taaagcgcag gtcaccatgc tggtgctcaa tggtttacgg
     1261 gtttaggaga tgcatgtgaa aacctttata acgatcacat tatttttaat aaccctgacg
     1321 gcgtgttcca aaaaaaacaa cacggccgat gcgtacggta attttgaagc agtcgaaact
     1381 ttgattgctg ctgaagccgg cggaaagctg cttttttttg atgtcgaaga aggggtcact
     1441 ttgagtgaac aacaacgcgt cggatgtatt gataccacac aactggcgct gaagcggata
     1501 caattggtcg cgtccaaaca aagtgtagac gcccgatcag ctaacgtcct tgcgcaaatg
     1561 gaagtactgc aggagcagcg ccgtgtggca atgacggaaa aaacgcgtat tgagaatctc
     1621 tatcgagaaa aagtagcgac gcagaaacaa atggatgaca tcacaggcca ggtgcgtgtg
     1681 atcgaaaaac aaatcgcttc cacggaagca caaaatgccg gcgtactgtc ggaactggcc
     1741 gggttggatg cgcagatacg tcagatcaac gatcaaatac aaaaaagtct cattatcaat
     1801 ccgatatcgg gaaccgtatt ggctaaatat gtacaatcgt atgagatcgt atcctttggc
     1861 aaaccgcttt ataaaattgc cgacatccgt gtgatgcatt tgcgcgcgta catcagcggt
     1921 gatcagctgt cgcatgtaaa aataggtcaa aaagtgcgcg tcgcgataga tgacaacgct
     1981 acggacaatc gctcgctcga aggcgaagtg atttggattg cctctaaggc ggaatttaca
     2041 ccgaagatca ttcagaccaa agaggaacgc gtcaatatgg tgtacgccat caaagtaaaa
     2101 gttcaaaacg atggcagttt gaaaatcggg atgccgggcg aaatgtattt tcaatgaagt
     2161 caggtgaaag attagaagtt ttttaaaatg aaaccgttaa taataaaatt acttatttcg
     2221 ttgtcggcga ccatctttgg actggcgacc atcacggcgg gcggcgctgc catttttttt
     2281 aatgagggtg ttttcggaac agtcatacct tgggttgtct ggtttaattt tttttcaggt
     2341 tttatctatg tcgcagcaag tctgtatgtg tggattgatc cgcgcgtagc ctacaaacta
     2401 agcatctaca tctttattac gacgagtgtg atttttgttt tattgggaat acatatcgca
     2461 tcgggtttgg cctacgaaaa aagaacagtt gctgcgatgg gcttccgatg gatagtgtgg
     2521 ggcattattt ctctgatcgg gtattatgtt tttgtgaaaa accaagcgga taataccaaa
     2581 ctatgatatc ggtcgaagcg gatcatctca caaaacgatt tggcaaaacg tcagcgatta
     2641 caaacctgag ttttcaggtc aaagccggag aactttttgg ttttatcgga ccggatggcg
     2701 ccggaaaaag tacgttattt cgcattctga caacactgat gatacccgac gaaggccgcg
     2761 ccaccgtgag cggattggac gttgttcgcg acttcaggga attgcgcaaa cgattaggtt
     2821 atatgccggg tcgtttttcg ttgtatcagg atctcagtgt agaagaaaat ctccaatttt
     2881 ttgcatcggt ttttggcacc tctattcagg aaaactacga tctcattcgc gacatctatg
     2941 ttcagatcga accgttcaaa aaacgccgcg cgggccagtt gtccggcggt atgaaacaaa
     3001 aactggcctt gagttgcgca ttgatacaca agccggaagt tctttttttg gatgaaccga
     3061 ccacgggtgt tgacgctgtt tcaaggcaag agttttggca catgctcaaa cgactccaga
     3121 ccaaaggtat taccatcatc gtatccacgc cttacatgga tgaagccggg ctttgtgatc
     3181 gtatcgcatt gattcaaagc ggtaaaattt tggatatcga cacaccggcc gacgtggtgg
     3241 gaaaatttcg ttcgccgctg tatgcgatca aaagtcgtaa gacctatctt ttgatggagc
     3301 atttgcgcgg atctgcggaa acacaatcta tttttccgtt cggcgaattt ttgcattaca
     3361 cggatcggcg tcccggggtt tccgaacagc aattacgtga tgaactgagg acggagggat
     3421 ttgatgatgt cgaagtgatg cgcaccgaac cgaccatcga agattcgttt atggcattga
     3481 tggagcaaca tgaaacccaa taaatccgat gcggcaatta cggttcgtga tttgaccaaa
     3541 cgctttggtt cgtttatcgc tgtcaatgcg attacgtttg atgtcggtcg cggtgaaatt
     3601 ttcgggtttt tgggtgccaa tggggccggt aaaaccaccg cgatgcgaat gatcatcgga
     3661 ttattggcgc ctacgtcggg tacggcacaa gtcgccgggt atgatgttta tacacaaacg
     3721 gagagcatta aaaaaaacat cggctacatg agtcaacggt tttcgttgta tgaagatatg
     3781 accgtgcaag aaaatatcac cttttacggc ggtatttacg gtttatccga cgcggcgata
     3841 cgtaccaaaa gcgaaatgct actggagcgt ctgcgcatgc atgatgtagc ccggactatg
     3901 ctgcgcgagt tgcctttggg atggaaacaa aagctggctt tctcggtggc attattacac
     3961 gatccggcca tcgttttttt agacgaaccg accggcggtg tggatccgat cacacggcgt
     4021 cagttttggg atatgatcta cgaagcggcg catcgcggtg tcaccgtatt tgtaaccacg
     4081 cactatatgg atgaagcgga gtactgtgat cgcgtgtcga tcatggtgga cgggcgcatg
     4141 gctgcgctgg acacgccgcg cggattgaag caaacgtttg aggcgcaatc tatgaacgac
     4201 gtgtttatcc gccttgcaag accgcagcaa agtaaagaat tatgaaaagc tttcgtgcat
     4261 ttatcgtcaa agaattcttt cacatccttc gcgacaaacg aacggtattg attctttttg
     4321 gtatgcccgt cattcaaatg attttattcg gctttgccat tcgtaatgaa atcaatgatg
     4381 cgcgcattgc catactggat catgcccacg atgcgcgttc acaggaagtc actcaaaaaa
     4441 ttttatcatc cggttatttt caaaatgttc aaacactgac gcacgatgat caaatagcgc
     4501 cggcatttga aaccggcgac gtaaagatgg tcgtggtttt tgagccgcaa tttaccgcgc
     4561 gtttgtatca cgaaggtgta gcgcatatac aactgatcac cgatgcgacg gaacccaata
     4621 tggctacgac gctgacagcc tacgcgactt ccattatccg tgattattcg gcgaatctta
     4681 atccgcatgc cccgacgccg atagtcacgg aagtgaaaat gcttttcaat ccggagatgc
     4741 gtagcgtata tctttttgtc ccggggctta ttgcgctctt gctgatgctg atatccgcac
     4801 tgatgacttc tatcaccatc acgcgcgaaa aggaacttgg gacgatggag attttattgg
     4861 tttccccgct gcggccgata cagatcatcg tcggaaaagt attgccttat ttgttgttgt
     4921 ccttggtcaa tacgtcaacg gttcttttac tggcacgatt tgttttcgaa gtgccgtttc
     4981 gcggcagtta cgcgcttttt tacgcggagg cgttactttt tatcatgacg gctttatcgc
     5041 ttgggttact gatttctacg attgcccgta cacaacaggt agctatgatg gcatcgttgg
     5101 ccggacttat gctgcccgtt attattcttt ccgggtttat ttttccggtt tccagtatgc
     5161 cggtgccgct tcagatattt agcaatattg ttccggctaa atggtttctc attatcgtca
     5221 aaggtatcat gctcaaaggc gttggtatcg aatatctgtg gaaagaaact ctggtgctcg
     5281 cgggtatgac acttttactc atgaccatca gtgcgcgaaa atttaaaatc cgattggagt
     5341 gacgatgcgg acgattcgat ttatcttgcg taaagagttt cttcagattt ttcgaaacaa
     5401 aggcatgttg cctattattt tcgttatgcc tttcattcag ctgttgatac tttccaatgc
     5461 ggcgacattt gatgtgaaga atgtgaaatt tcaaatcgtt gatcgcgacc ggtcggattt
     5521 gtcaaggcaa ctgattgagc gttttacgtc aacgcatcat tttattctca cggacatgcc
     5581 gacccattat gcgcaagcgg aagaatctgt tttaaaaaac gaagccagtt tgatcgttga
     5641 aataccagtt tttttcgaaa aagatgtgat cacgggagcg acggtgaaat tgcaattagt
     5701 gatcaattcc gaagatggaa gtacggccgg cgtgattcag tcatatgcgg ctcagatcat
     5761 tcaggatttt accagacaag tcgtgccttt ccagcagaca cagggcatga cgcagcgcat
     5821 cgaaataatc gccagaaatt ggtataatcc ggaattgaat tataaacatt atatgatacc
     5881 cggcatactt gtggcattgg taacgatgat cggtctgttt ttgtcaggca tgaatgttgt
     5941 gcgggaaaaa gaaatcggta cgatcgaaca aatcaacgta acaccgataa aaaagcatca
     6001 attcatcata ggaaaactgt tgccgtttct catcatcgga ttatttgaat tggctatggg
     6061 attggctatt ggccgcttag tgtttgattt accgatgctc ggcggattgc cgttggtttt
     6121 tggcatcacg attatttatc tgatattggt tttggggttg ggtcttttta tttcgacggt
     6181 tacagaaacg cagcagcagg cgatgtttat cgcatggttt tttatggtga tttttatgtt
     6241 gatgggagga ctttttacgc cgcttgacag catgccgtta tgggcgcaac agaccatgtg
     6301 gatcaatccg gtagcgctgt ttattcgcat actgcggcag gtgttgctca aaggcgcgcc
     6361 gctacatgat ataattcctt atgtttccgc gctgatagtt ttagctgtca ccatgtttac
     6421 attggccgtc attcgttatc gtaaaacaag tgaataacga taatacttgt ttccaatcct
     6481 caattttgtt tatactctca atgcacacaa agaaaaatga ttttggagtt agcttatgaa
     6541 gtacatagtg attttttttg tattcgtaat gacgatagtg agttgcaaac ccgggccgaa
     6601 accggctccc gaaaatgcgc ccgcaggtaa tccccatgcc ggaaatccgc atgcaaatat
     6661 tgatatgaat gcaccgggcc atgtagtttt taaagcccct gaagtttgga agtctgaaac
     6721 gccgaccaat tcgatgcgta aggagcagta ccgtcttccc ggcagtaaac cggaagatga
     6781 tgcggaactt gtattgtctt attttcccgg cgccggcgga tccgttgatg ccaatctttc
     6841 acgttggtac gggcagttta aacaaccgga cggcggtgat actcaaagta aggccgtaat
     6901 caaaaataca gaaaacaaca aactcaaaat aacggttgtt cgtgtgagtg gtaattatat
     6961 gaaaccgcgt gtggccggtc agatgggcgg cccggtggat gaaacgccgg cttacgcgat
     7021 gtgggcggct atcgtcgagg cgcccaatgg tccttggttt ctcaaagcgg tcggccctca
     7081 aaccacgatg gacaaataca gtaaggagtt tgaacaactt atcggcacga tagactatat
     7141 ccttgattaa tttttactga actcggatcc catctcgaga atacgtacgc gggctcaccc
     7201 atcatttcag gcgagcccgc gtttttttat gggaatttac gactttctac ggattgattt
     7261 ttaatcggtt ttattacttt aaaacgcata aaattaattg ctataaaaag cctctacccg
     7321 tatattgccg cctgatgaaa tcaaaaaaaa caggcgacga atttagcctg attcgtcagt
     7381 tacacgcgat tctggagtcg ggttatccga ccggaaaaaa tgttctttgc ggcataggcg
     7441 atgatgcggc atgggtgcgg tcatcggcaa aacaacaagt tatcacgacc gatgcaatgg
     7501 tcgaaggcat tcattttgac cggcgatata tggcgtttga agatattggc tataaaagta
     7561 tttcagtcaa tgtcagtgat atcgcggcca tgggcgcaga tccttcattt gcagtgatcg
     7621 cgcttcaaat ggacccgcag acgggtacta aaaatataca tgcgttgtat cgcgggataa
     7681 caaaagcatg ccggaattat aatgtacaaa ttgtcggcgg aaacatttcg cgcagttctg
     7741 cgttttcgat aacggttact ttaatcggaa acgttgctga aagaaataga ccatggcttc
     7801 gcagcgatgc ccgtatcggg gacgtgatcg cggtaaccgg cgatcttgga ggatcggcgg
     7861 ccgggttgat gttttatgat cgtttaaaga aaaaaccggc gtatgcctgg caacggcact
     7921 gcagaccgga tgcgcgactg gaatgggcgc aaatactgag aagaaaaaat attcgtgtgc
     7981 atgccgcgat agatattagc gacggattat catccgattt ggcgcatgta tgccgggcca
     8041 gccgttgtgg tgcgttaatc gaagccgcat gcatcccggt acacgcggaa ataaaaaaat
     8101 taagcgatgt gctgaaaata gattctatga tgtgggcatt gcatggcggc gaagattatg
     8161 agttattact taccatgtct cctaaggaat ttgtcaaagc aaagaaaata ttaaaaaaca
     8221 acatacatgc catcggtacg atcgtcgccg gcacatcggt agtgatcgcc gatgcatccg
     8281 gtaatcgtaa aaccttagaa ccggcgggat ttcgacattt ttaacgagga tattatggca
     8341 caatcaactg gtaacatcaa tgagcgcatc gagagaatac aaaaagatat tgcgcgtaac
     8401 gggtacgacg ggtggctgct gtacagtttt agaaaaaaca atccagtcgc atctagcatt
     8461 ctgagcatac cggaacattt gacgcaaaag cgccgatttt tttattacat tcctgcgcaa
     8521 ggtacaccca aaaaacttgt acacggcata gagcgttatg ttttggatac gttgccgggt
     8581 gaaaaagtga tcttttccag ttggcaggat cttgaagccg gattaaaggc catcaccgcc
     8641 gggcataaaa aaattgcgat ggaatattcg cccgaatgta acattccgta tgtggctatc
     8701 gtggatgccg gtacaatcga attggtcaga aaaaccacgc aagccgaaat cgtttcttcc
     8761 gccgatctgg tccaatattt tgattcgact tgggatgatc agcaattgca attgcacgtc
     8821 gaagccggta aagccttgac ggatatcatt ccgcaagtgt gggggtttat tcgtgaaaaa
     8881 ataaccggtc gccaaaaagt caccgaatac gatgtacagc aatttattca caaccaattt
     8941 atcgaacgcg ggttgaccac gggcgacttg ccaaattgct ctgtcaatga aaatgccggc
     9001 gatccgcatt atgagccgac aaaagatcat tgtaaagaaa tccatgaagg ggatttggtg
     9061 ctcattgatt ggtgggccaa gaaaaatcat ccgcgcggcg tttatgctga ttatacgcag
     9121 atgggttttg tcggtaaaac ggtacccgaa aaattcgcca aagtgtttga tgtagttgtc
     9181 ggcgcacgcg atgccgcgat cgcttttata aaagaagaaa tcgccgccga tcgtccgctt
     9241 ccgggttgga aaatcgatga tgtttgccgt gcgcatatcg tccagcatgg ttatggagaa
     9301 tatttcattc accgtaccgg ccactcgatc ggtgaagaag tgcatggcaa cggcgccaat
     9361 attgataatc ttgaaaccaa agataatcgc cgcattattc cgcgtacatg tttttcgatc
     9421 gaacccggaa tttattttgt aggcgacttc ggcgtgcgta ccgaagtgaa tgtgtatgtc
     9481 ggagacaaaa aagatgtttt ggtgaccggc acaccgatgc aatcatccat attccctatt
     9541 ttagcgtaac gaaaaggagt ctgtcagcga atgtccaagt tctatgtgac ccacgagggt
     9601 ttattaaagc tcaaagaaga gattcaacat cttaaaaatg ttaaacgtcc cgaaattgcc
     9661 aaccgtgtcc aggtggcgcg ggagcacggg gatctcaaag aaaatgcgga atatcatgcg
     9721 gccaaagaag agctgcagtt attgcaaagc aaaatcaatg aaatggaaat gaaagtgatg
     9781 aatgccgtca tagtcaaaga agacgaaatt cccactgaca aagtatatat ccttgcgacc
     9841 gttgatttgc aaaatgttaa aaccaaagaa aaactgactt atacgttagt tccggataat
     9901 gaagccgatt gggaaaataa caaactatcg gtgaatacgc ctattgccaa aggattgctc
     9961 ggaaaaaaag tcggcgatgt ggccaaagtc aaaattccgg ccggcgaact cgaactcaaa
    10021 atcatcaaaa tcagccgata aattatgaat gaagatacgt cacaaccgga tatacaagat
    10081 gaagaaacat ccggcgaagc cgcatccgat acgcgtcgca aacgtatgtg gcagattttt
    10141 ggttggtcgg tcatcggagc gctccttctt ttgatcgtgc ttaacctgtt tcagtgggcg
    10201 tcaccggaaa tcaatcagta tggtctgcca gtgatcgttg tgttatacgt cgtatatgtt
    10261 ttttttagaa aacggtagat tgattatatg aaaataggcg taacgtgtta cccgacgtat
    10321 ggcggcagcg gtgtcgtagc gaccgagttg ggcatcgaac ttgccaagcg cggccatgat
    10381 attcatttta tcacctccga tattcctttt cgtctcaata cgtttcgtga ccgcatttat
    10441 tttcatgccg tcgaagtgat gaattatccg gtattcgatc attcgccgta ttcgctttca
    10501 ttggcggcac gtatggctga agtcgcggaa tttgaggatt tggatttact gcatgtacat
    10561 tatgccatac cgcatgcgcc aagcgcgtat ctggcaaaac agatcatccg aaaaaacaat
    10621 cgcatcaaag acttaaaaac cgttacgacg ttgcacggaa cggatattac attgctgggc
    10681 atggatcgtt cgtttttaaa tctgattaag ttcagtattg acgaaagcga cggtactacg
    10741 tcggtgtcaa aatatctgcg taaccgcacg tacggcgaat ttaagcccaa ccgaaaaatc
    10801 gaagtaatac ccaattttgt cgatacggaa aaatatcgtc cccacgataa acccgaagac
    10861 agttcacatg gttttttgag ccgcatcacg cccaatcacg aaaaactgat catgcacatc
    10921 agtaattttc gtccggtcaa gcgtgtcggc gatgtggtga aaattttcgc caaagtaaga
    10981 gaaaacatta atgccaagtt gctcttgatc ggtgacggtc cggagcggcg catgtgcgaa
    11041 cagctggttc aagatttcgg attggatgag catgtcgttt tcctgggtaa acaagaagcg
    11101 gttgcggaat tgatttgttg tgcgcatgtt tttcttttac caagcgaaga agaaagtttt
    11161 ggtttggccg cactggaagc tatggcggcg ggtgtaccct gtgtcacctc caatgccggc
    11221 ggactttcgg aagtgaatat tcacaacgaa accggatatc tcgaaaaaat cggcgatgtc
    11281 gatgcgatgg ctgcggatgt agttaaaata cttacggatc aggacgtgta tcagcgattt
    11341 cgcgccaacg cacgccaacg ggtcgtggat aatttcggaa cggatcgcgt cgtgcaacag
    11401 tatatagatt attaccaaaa agtcatcgaa ggcaaaaaca cgtgatcgct ccgaagcata
    11461 ttatgatcat ggcgcaccgc ggtgtgacgt attccggctt tccggaaaat tcgttaccgg
    11521 gttttcaggc tgtcgtggat gaagggtatg acggtgtcga actggatgta cgcctgacac
    11581 gcgataaaga attggtggtg tttcatgata ttcgccttga ccggcttacg accacaggac
    11641 gcggcatggt gcgaaccaaa acattggaag agttaaaaaa acttacgttg cgatcggacc
    11701 gtgttcaaac aggcatccct acgttgaagg aagtgttgac ggtttttcaa ggtaaacctg
    11761 tttttgtaaa tatcgaaatc aaaagcgaaa tgcccttgcg cggaaaaatc gaagagcggg
    11821 tcattgattt gatttacaat atgcacatgc gcaaacaagt ggttatttcg tcattcaatc
    11881 cccttgtcgt caaaaaatgc cagcgtattg atcccgacat gcgcatgggt tttatttatg
    11941 aaaaaaggct acccaatctc aatcatcgct tagccaaagg gcttgttgta gattcatggc
    12001 atcccaattc cgtcggtgtc aatgcattgc tgatggagcg agcgtgggaa gcgcgttgtg
    12061 cggtatatcc ctggacggtc aatgatgaaa aagaaatgca acgcatgatc gatctcggtg
    12121 tggacgggtt gattaccgat taccccaaca ttgcaaaaaa aatattacag aggatataag
    12181 gtgaattcaa acaaaagcaa agcattgctt gtgctggaag acggcacatt ttatgaggcg
    12241 tggtctttcg gtgcggatgg cgaggcgacc ggcgaaatcg tatttaatac cagcatgacg
    12301 ggttatcagg aaatcgtctc cgacccgtcg tattacggcc aaatgatcgt catgacgtac
    12361 cctttgatcg gtaattatgg gatcagcgac gaggatttcg aatcgcgcgg accgcaagcc
    12421 aaagcgttga tcgtcaaaga agtttgcgat tatccgtcca attggcgctc atcccactcg
    12481 ttatccgatc tgctcaaaaa aaacggtatc atgggtatcg agggtttgga tacccgcgct
    12541 atcacgcgcc atatccgcaa cttcggcgcg atgcgtgcga ttatttcgac gacggacaca
    12601 agcgtgcaac ggctgttgga aaaagtgaaa aatgcgccgc ggatggaagg tgctaaccat
    12661 gtggatgccg taacttgcaa aactaaatat caatggtctg aaccgaatgc ggaaatgtgg
    12721 cagttccgtg aaaatattac gggaacccat ccgtatcgaa tcgcggtata tgattttggc
    12781 gtcaaacaaa atattttgag aaaatttgtc gaacgcggct gtgtggtcga agtatttcct
    12841 tccaaaacaa aagcggatga aattttggca agtaatccgg acggaatatt tttatccaat
    12901 gggcccggcg atccggaagc cgttgattat gtaatccctg aaatcaaaaa atttatcggt
    12961 aaaaaaccgg tgttcggtat ttgccttggt caacaattgc ttggaatcgc tttaggtgca
    13021 aaaacatata aattaaaatt tggccatcgc ggagcaaatc atcccgtaaa gaatttagcc
    13081 acagggatca tcgagattac ttcgcagaat cacggtttta ccgttgatcc taaaacattg
    13141 aatgagaaag aaatcgaact tacccatttc aatttatatg acggcacact cgaaggattt
    13201 cgacatcggg agctgccgct tttttccgtc caataccatc cggaagccag tcccggaccg
    13261 catgacagcg attatctttt taatcaattt atggatcgcg taaaagcgga taagaattga
    13321 ttgatgtacg cgctgttgga agaaatagaa aatgccatcg cggctcacga gctgtggaaa
    13381 gaacgtctca tggcggcgat tgaaaccggg catagtgtgt gggctgtgga taaagtgtcc
    13441 gttgatgatc aatgcgcgtt tgggaaatgg attcacatga tcgaagcgga ttacaaaggc
    13501 gatcattttt acactacggt tcgcgaattg catgcgcatt ttcataaaat agccggtatc
    13561 gttttgaatc tggctctaag cgggttcaaa aagcaagcca ctgaaattta tcagggcgaa
    13621 tatcaattgg cctcgcaaca tcttctcttt gaattgcacg cttggaaaaa atccttatag
    13681 gttttacagt tcctctgtta aaatattaac cctaacacaa ggagtgtgcg tatgaaaatc
    13741 catcgtatcg ccgtcatggc tttggctgtt ttgatgagct gtcaaccggc caaaaaagaa
    13801 ccggcaaaag cagaagcaac gaccgtcgcg gctaagaaca ttacgggtga agagatcagt
    13861 tatgcggcgg ataatattac catgaaaggt tttatcgcct atgatgcagc gcaaacgggc
    13921 aaacgtcccg gtgtgatcgt cgtgcatgag tggtggggtc acaatgaata cgcacgtcaa
    13981 cgcgctaaaa tgcttgccga aatgggatat gtggctttag cggtagatat gtacggcgat
    14041 ggaaaaaaag cagagcatcc gcaagacgcc ggaaaatttg catccgaaac tatgaagaac
    14101 ttcaaaggcg ctaaagcgcg ttttgaaaaa gcggtcgaaa cattgaagaa taatcctaat
    14161 gtggacgcat cccatatcgc ggctatcgga tattgttttg gcggcggcgt agtgctcaat
    14221 atggcccgtc agggtattga tatgaaagcg gtcgtgagtt ttcacggaag cctgggtgca
    14281 gcagagccgg cgaaagaggg ggtagtgaaa agtcgtgtcc tggtatgtaa tggcgctgcc
    14341 gataaatttg tttctgaaaa agatatcgaa ggctttaaaa aagaaatgga tgccgctaaa
    14401 gtcaactacg tattcaaatc ctatgacggc gccgtacacg gctttactaa tccggcggtt
    14461 gattcactgg gtaaaaaatt caacttacct ttagcctata atgctaaggc cgacgccgat
    14521 tcatgggcgg atatgacagc gctttttaaa gaagtgttcg gtaagtagtt tgatatgtac
    14581 gaataacgat tgtcccaaaa gtgaaatatt ttattccgtt tttatttaac gggtacgtat
    14641 tagaactttt gggacatttt tataaaaatc atttggaaat attatgacca aaaaactacc
    14701 gctgcaaaag catctttttg atatccccga tgaggtgacc tttctcaatc atgcgttttt
    14761 tgctccgcaa cttcgtgcca tgaccgaagc cggtttacaa tcggtacgtg caaaatcgtc
    14821 gccatggaat tatacgccgc aacattggtt tgaagatggc gagacgttac gcgggcttat
    14881 cgctcagatt atcggcgtag catcggatga tatggcgatc attccttcag taagttatgg
    14941 catggcgata gcggcaaaaa acataacggt gcgttcggac caaaatatag tggttttgca
    15001 cgaagagttt ccctcaaatt attacgcatg gtattataaa gcaaaagatt gcggtgcatc
    15061 gctacgggtc gtacatagaa ccaatcagtt gaattggaca gaagcgatct gtgaaaatat
    15121 tgatgaaaaa acagccgtag tagccgttcc taattgtcac tggacggacg gtgcacggat
    15181 tgatttggag cgtgtctcgc aaaaagctcg ttcagccgag gctgcactgg ttattgatgc
    15241 gagccaatcg ctgggcgcat ttccgattcc tatggaaaaa atccgccctg attttttgat
    15301 ttcggtcgga tacaaatggt tgctcggacc ttattcgttg ggatatttat atgttgatcc
    15361 gaaatggcag gaaggcactc cgatcgaata ttcttggctc aatcgttttg gcagtgaaaa
    15421 tttttcccgg ttaatcgaat acacggacaa atttcgtcaa ggtgcgcggc gctatgacat
    15481 gggtgagttt tctaatttta tcaatgtgcc catggccatc gccggattac gtcaaatcat
    15541 aacatgggaa gtttcagcaa tatccgaaag cctgcttgat ctgacatcgt atgctgcgca
    15601 atatgcacat agtttaggtt gggtaacgat ttccgatagt gaacgcattc cgcatttgtt
    15661 gggcattcgt catgcaactt cgatgccgtc tgacatcggg cagaaattat cagaaaacaa
    15721 gatatacgta agtattcggg gaaacgctat tcgtgtcgct ccctatcttt ataatcaaaa
    15781 aagcgacatg gatcgtcttt ttgaagtatt aaaagtgtat agccgctgat aaaaaaatcc
    15841 cgacgtgtta tacatcggga tttgagaggg atttatgcta accgtaaaaa cggtgtttta
    15901 gttttcagct gttctttgtt tgcggggaac tttaagtgtt agcgacgaaa atgaacgacg
    15961 gcaacgaaaa acgaaagcca cgtgatcatt ttcattgtta tatcctagaa aatcgtagtc
    16021 atccatattt tcagaataat atttcattcc tagttcacgg tttttttcat tagccgcaat
    16081 ggataaatgt ttacgtagcg cttccgataa tacaggtgtt gactgcgtca tgtgagtttg
    16141 cacaaactat tttattcgag agttgtagtt tttcgatgat aagaaccaat taccatcatt
    16201 cagcgctaac atacgcatat ctacgtaggc ttcacaatac cataaatacg gtatattgtt
    16261 aagattgatt ttatgaaggc tgggcttggc ggttaggtga gaaagatcag ttgcttcgct
    16321 cgtatatatc aatgaacgtt ttgatgccgt ccacgatacc ttcggaaagt tttttctgac
    16381 ctgcttccga tttgagaaat ttttcttcgt ccggattgga tatgaatgct gtttcgacga
    16441 gtatcgaagg catggaggcg cccatcaata cataaaacgg cgcttgcgtt acgccgcgat
    16501 tggtgatgtc cacttgttta tcaatacctt tggaaacata agaagctaat tcttcgcttt
    16561 ctttgacgaa aacactttgc atgatattgg cgagaataaa tttttcgtcc gtaaaatcgc
    16621 cgtaatgatg tttatcgtct tccatatcaa tcacttcgtt ttcttttcgc gcgacagcca
    16681 acgcttcggc attgcggctt ggcgatagaa aaaagatttc aaatccatgg gccttacggc
    16741 ttttattgga gttacaatgg atactgacga ataatttagc gttcttttga ttggcgaatt
    16801 gcgtacgtcc tttaagcgtg atgtacgtat ccgtatcacg tgtgtattcg acagggattt
    16861 ttagccgctc tgcaatgatc ttacccactt tgagggcaat gttcaacgta atatcttttt
    16921 ctttcgtccc acctttgccc gaagcgccat gatctttgcc accatggccg gcatcaatca
    16981 caattttatt gatcttccat tggtctttat caatcgcacc ggtccctgat ccgccattgt
    17041 caaactccag atcgctgctt tgatgaatgc gccatgcgac gtaaatctca tccttcttat
    17101 catccacgga gatatcgtaa ccggcaatat cggaattaag ctgaaatgaa aactgtgtcg
    17161 agtttttatg ctgaaatatc aatgcttctt tgatcagttt gtactgttcg gatagtttga
    17221 tgtctttggc caacaagtcc gaatgatacc cgccggagac cgtcacgtat aaccagtttt
    17281 tattacgcca aatttcaacg tctgacgatt gaaagcgccc gtccgttttg atgcgcatca
    17341 aaagaccgtt tttcttcttt tcgaaaataa cgtctttcag gtttggggaa atactgacag
    17401 ccgaggtttt aatcgtatct ttgattgccg gtgcggattt tgcgattatt ttttcgccat
    17461 tgatgacgac cggcgtagag agatatgcat tgatagcact aaaaaaataa acagccggaa
    17521 caaagatcgc gccgtctata tactgcgtgg taaccggcat ttgtaaggtt gttttttcat
    17581 ctacgacgac aaatgcattc aaacctgtaa ctttaaattg gtgctgatta gaagtaaaca
    17641 ccattttctt tttggcggta ttttcaaaaa aaccggcatc cagcagtgcg atcacgtctt
    17701 taatagaaac gtattcctga tcacccgtga aaacaggtat gacttctttg ttagccgtac
    17761 cgtaactgag tttcatcggt gcttgagcca acgcgatgac tccgccgata cttgctgcaa
    17821 taatggtgca aaaaaatata tggta
//
LOCUS       DAOQNQ010000038        17568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1140_length_17568_cov_5.468338, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000038 DAOQNQ010000000
VERSION     DAOQNQ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17568
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1140_length_17568_cov_5.468338"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..762
                     /locus_tag="PK754_04260"
     CDS             70..762
                     /locus_tag="PK754_04260"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNB08749.1"
                     /translation="MKYLVWLSLLISFSPVYMGDVCAQGKIGYVDFQYLITQIQSVHD
                     VQTELEKLSRDWTDRLSAMEDSVALIEKDYEALSITLTKSGKNVLEKNIADAKQKILV
                     YREQKFSPVTGELYKKQQELLQPILDKVKRAIDNVRLKEKYDVVFDISTGNPVSIDKR
                     LDLTLLVLEELPSVGLTVAQVEGQGTGTNLKTPGSNPTNRTPSGRSSKSTTNETSKPV
                     KENDTDPAIKDN"
     gene            793..1344
                     /locus_tag="PK754_04265"
     CDS             793..1344
                     /locus_tag="PK754_04265"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNB08750.1"
                     /translation="MKKTMIAILMLTLGINTAVKSQGKIGYADSQKILQSLKETETVQ
                     TKLQAEQERIYKQFQYMQDSLQTMQEDYVKNYRDNPLIKDNIKQTFQKGMEELAYLIQ
                     NSQQKYQEEMYKKQQELMAPILDKVKKAIETVRKAKGYDMILDSSPGMILAYDPQLDM
                     TQKTIDELIRMATAAPTTGTGGK"
     gene            1415..2428
                     /gene="lpxD"
                     /locus_tag="PK754_04270"
     CDS             1415..2428
                     /gene="lpxD"
                     /locus_tag="PK754_04270"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNB08751.1"
                     /translation="MASRSYKFSDIAKLLERPFVGSDSEITGFNEPMYALESEVIFLF
                     QKNYVPQSVSGASKFWIVSEKVFAKDLAEQCLKKKTAYILSKDPYEDIIKVLTWWIDQ
                     RALRRGNAFKIKSKLYNGVKTHRSSVIGYDTIIGRGTLISAHAVIGNHVRIGKGCVIY
                     PNVSIYDNTIIGDRVIIHAGSVIGSDGFGFHKTEEGYLKIPHIGRVVVGDDVEIGANC
                     CIDRGTVGETKIGRGCKLDNLIQIGHNVIIGPHTVIAAQAGVAGSTRIGDHVTIAGQA
                     GLVGHINIGDHAVIAAQAGVIGSVDENTTVSGYPAREHRAALRRDATLKKIIEQYDQK
                     DKV"
     gene            2434..3477
                     /gene="ruvB"
                     /locus_tag="PK754_04275"
     CDS             2434..3477
                     /gene="ruvB"
                     /locus_tag="PK754_04275"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNB08752.1"
                     /translation="MQDITDDGLARPRKVPEDLDLDISLRPSRFDEFVGQQRIINNLK
                     VFIEAARLRGEALDHVLLSGPPGLGKTTLSHIVAHELGVHIKITSGPVLDKAGDLAGI
                     LTNLEERDVLFIDEIHRLNPIVEEYLYSAMEDFQLDIVIDKGPSARTIQLTLPHFTLI
                     GATTRAGLITAPLRSRFGITSRLDYYNDKDLAHIVTRSAEILQVDIDASGANEIASRS
                     RGTPRIANRLLKRARDFAQVRGKKIIDKEIADYALKALEVDEHGLDEMDKRILSNIIE
                     RFNGGPVGIDNIAIAVGEESGTIEEVYEPFLIQKGFLKRTPRGREVTKLAYDHLRIRP
                     RKTSQQAGLDLEE"
     gene            complement(3490..4122)
                     /gene="thiE"
                     /locus_tag="PK754_04280"
     CDS             complement(3490..4122)
                     /gene="thiE"
                     /locus_tag="PK754_04280"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNB08753.1"
                     /translation="MNKRLNVSLYFIADVGMIPAGKICSVVEEAIQGGVTFVQLRAKT
                     LSVNAMIALGRTIHQVTYSKNIPLIINDRIEVATAIGAEGIHLGQEDITVGAARKILG
                     DSAIIGISTHTQQEAQTAESAGADYIGVGTVFPTASKKDIVGIIGTTGLKQIRDLVKL
                     PVVAIGGINQNNVKEVLACGVDGIAVISAIGLSVNPRETTRALKDLLTVN"
     gene            complement(4187..5899)
                     /gene="sucB"
                     /locus_tag="PK754_04285"
     CDS             complement(4187..5899)
                     /gene="sucB"
                     /locus_tag="PK754_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HNB08754.1"
                     /translation="MAKVDMVMPQMGESIAEGKILKWHKKVGDKISKDETVLEISTDK
                     VDSEIPSPASGIIAELIVPEGTTVAVGALIARIETDANASVASPAPTPSPKVESKTAP
                     VAQNTPASKPASGGASVDMVMPQMGESIAEGKILKWHKKAGDKVAKDETVLEISTDKV
                     DSEIPSPASGVITEIIVPEGTTVAVGTLIARIGAEGATVAASTTAAPTPKTEEKMHFS
                     SSIEDFQKQNQIAATAATPAATVNAGGGMVKGESNRFYSPLVRSIAKAEGLSMQELDS
                     IAGTGLNHRVSKNDVLAYLAKRPKGGVRPAVTTTTSASTAAAPAKQVSDVKYGYNKER
                     SELIEMNNIRRITAEHMRRSIDTSAHVYSMTEIDMSRVVRFRDKYKSAFEKREGFKLT
                     FTPFLIDAMVRAIKDFPMINISVDGNTIVKKNYTNVGMAVSIENNTALIVPVIKDADA
                     MNLVGVCRATNDLALRARSKKLRPEDIQDGTITLTNMGVFGTTVGFPIINQPQVAIMG
                     VGTIKKRPVVITTDDGEDMIVIRPIMYASLSYDHRVVDGALGGQYLQRVAYHLENFDE
                     NQTI"
     gene            complement(6005..6775)
                     /locus_tag="PK754_04290"
     CDS             complement(6005..6775)
                     /locus_tag="PK754_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNB08755.1"
                     /translation="MDYTTIRVEHHAREAHVTLNRPPVNVINLSMIDELNVALDKITS
                     NPDVTTIVFRGAGDKAFSTGVDIKDHTLDKINSMLKSFHHIFRTLANSDKITVASVHG
                     YCLGGGMELATFCDFVIASDSTIFGQPEIKVGCYPPVAAAWLPQKIGLRRAMEICMLG
                     ENIKAHEALTLGLINKVVPPDELRSATDTLLQNLNALSGTVLKQTKRALLAGADKPFT
                     TALDTAENIYLQELVKTHDIEEGITAFMQKRKPEWKHR"
     gene            complement(6789..7733)
                     /locus_tag="PK754_04295"
     CDS             complement(6789..7733)
                     /locus_tag="PK754_04295"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNB08756.1"
                     /translation="MRSLFLLILTGLMISVNSCHWFVVKPQFDGQSAMNYLKKQCEIG
                     PRNPGSEGHQKALEYMTEELRKYCDRVDHQKFHYTDKRDTTKKFELTNIIASINLNPK
                     NKKRVLLCAHWDTRPWADKDPDPANRMKPIIGANDGASGVAVLLEMARTIKQQPLDIG
                     VDIILFDLEDYGDHNWESKPDSLNAYCIGSEYFAKNNKTYFPEFAILLDMIGDKNLDL
                     PIEQNSYTGAKDIVNKVWDAAKRLEKPVFRKTIESNVYDDHIPLLRIGIKCIDIIDFD
                     YPDDSNRYHHTMDDTPDKCSAESLQQIGDVLVEVLYNE"
     gene            complement(7746..8591)
                     /gene="folE2"
                     /locus_tag="PK754_04300"
     CDS             complement(7746..8591)
                     /gene="folE2"
                     /locus_tag="PK754_04300"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HNB08757.1"
                     /translation="MSAIEDTQGRDDFRKMPIQRVGIKNLKYPLTFINAAGRRTPTVA
                     MWGLAVDLPHDKKGTHMSRFGEILNAWQDGLSIESMPYFMNMMRERLHADRAFARVTF
                     AYFIEKEAPVTLTKSFMDYTVAIDASSIHDKMTLSLEVQVPVTTLCPCSKSISEYGAH
                     NQRSLVTVKAECETDNFLSIEELVYSVETCASSELYVVLKREDEKYVTERAYDHPAFV
                     EDLVRDIAIKLSQDARIVRYRVDVTNLESIHNHDAYAVIESQSVSSVLSDNGAKHHAS
                     PEQQS"
     gene            complement(8612..9379)
                     /locus_tag="PK754_04305"
     CDS             complement(8612..9379)
                     /locus_tag="PK754_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNB08758.1"
                     /translation="MFLHATTIYGPLTTRRLGTSLGINLLPVGRKVCNFECVYCECGW
                     TDTSVKDAVPTLEAFTEALESKLSALRQQNQNIDHITFAGNGEPTLHPNFSAIIDTTR
                     EKREKYFPNARIAVLTNATRVHKTEIFDALKKTDDPILKLDAGTETMFQRIDIPDKGI
                     SLDGITETLKKFNGNLIIQTLFLRGEHQNTVIDNTTPEEVHAWLRRIEAIKPRKVMVY
                     PIDRDTPVKTLEKISATELEKIAAQVRTLGIPAEVFG"
     gene            complement(9413..10960)
                     /locus_tag="PK754_04310"
     CDS             complement(9413..10960)
                     /locus_tag="PK754_04310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNB08759.1"
                     /translation="MKKEIIINATDAETRIAIVEDERLVEIFVERPEKNRMVGDIYKG
                     RVAKVFPGMKAAFVNIGMKQDAFLHFSDIGDNTHEYAVINDADDADDNEAPVKPFNHP
                     RIHNHDNHGRFLRVGQEILVQVIKEPIYNKGARVTSEISIPGRFTVLIPNDPNEMIGI
                     SRKINTVPERKRLRRLAKEVKPRDFGLIVRTNAEERDEEVLRNDIRNCLDTWHDIDTK
                     AQKISAPNLVYKDMEMVSSVIRDLFSNDVSRVLIDDKKLYKSISNYISTTSPQLGNRI
                     ELYRSNKPIFDLYNIEQDLQQSIEKKVPLKNGGYIIIEHTEALISVDVNSGKYIGRGS
                     YEENSMAVNMVAARETCRQLRLRDIGGLIVIDFIDLHDERNKKKLFEEMKKELRKDRA
                     KFSILPMDEYGLIEMTRERIRPSIMFSLSEPCPACSGTGRVGNRTTLFDKIERWARRY
                     KEKNSFFSKLEFVVNSDILEQVKPKIKNMRWRYLIFSRVTGEQEIRMDEFRVLQKNED
                     ITEMYGS"
     gene            complement(11234..12769)
                     /locus_tag="PK754_04315"
     CDS             complement(11234..12769)
                     /locus_tag="PK754_04315"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HNB08760.1"
                     /translation="MSDIVLTDNDLALLAQIGKSPEQLSAEIEFFKTGFPFVELDRPC
                     TIGDGITVLSDSTHDALIKKADDAAAAGRVKKFVPASGAATRMFKSLMAVHYDRMLQQ
                     NTSHPDTRFLKTFFDRLTQFAFYDELDGVLAKDGLSLETLRQTRSYSDVLSYLMHDVG
                     LDYAHRPKALIPFHRYPEGARTALEEHLTEALAYATDTDQNAYVHFTISSEYRQLIDS
                     HIQTVRSRYETSGRRIQIEISEQKPSTDTLAVDLHNRPVRDQDGRLHLRPAGHGALIE
                     NLNDLAGDIVFIKNIDNVVPDRLKEPTFRYKKILCGHLIEMQLEIFGYLKKLKNNPPN
                     SDTVTEAESFAISRLGMQASSTMDIRERRDNLMQFLNRPIRVCGMVRNQGEPGGGPFW
                     VREPNGNRSLQIVETAQIDLQWPKQKLILDDSTHFNPVDIVCGVRDFEGKPFDLHRFI
                     DPHTGFISKKSKDGREIKAMELPGLWNGAMAKWITIFAEVPLITFNPVKTINDLLRPE
                     HQP"
     gene            complement(12772..14274)
                     /locus_tag="PK754_04320"
     CDS             complement(12772..14274)
                     /locus_tag="PK754_04320"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002077.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNB08761.1"
                     /translation="MHVSAKYSKLKHWKSELTCVFIAKNEPVNFWKNELEDIYEDTIP
                     KLLKSKEFGAEEEKVFLLLGNDKTSSKRIALIGLGEYKDLEPETLRRTAAKAVRTALA
                     MEYSKITLAFPAHLRQKNWTDDRIAQALTEGACLAHYKYDKYHTDKKPQDRHRITELT
                     LIADHEPTAALQQGVRAGEIISNAVNFTRDLGNAPGNELPPRELADRATAMCRTHKIK
                     CTVFNETKIKSLKMGGILGVTAGSAEPPRLVTMEYKPAKPKNTKPIVLVGKGLTFDSG
                     GISIKPADQMDRMKFDMCGGAAVIGIMQAIAELKLPLYVVGIVPSSENLTGSRAYKPG
                     DVLTMYSGKTVEIINTDAEGRLILADALAYAQRYKPEVIIDYATLTGHCVIALGMHGA
                     GLFATDSNIKQKLMHASDASGEKIWELPLWKEFDKQIKSHIADIKNTGGRPGGASTAA
                     AFLKHFVGNTPWAHLDIAGTANSEEELPYTDRISATGFGVRLTVEMLRHW"
     gene            complement(14277..15299)
                     /locus_tag="PK754_04325"
     CDS             complement(14277..15299)
                     /locus_tag="PK754_04325"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNB08762.1"
                     /translation="MATKKAKKKNMFDAIKPVIAKAKTFVLTTHVNPDGDGLGSEAAM
                     YLFLKSLKKSPRIINTSPIPKAFQYLNYGNIIETYEPAKHDKVIQKADVVMVLDISVS
                     KRLDRMQEAVVQAPGTTICIDHHLDNDGFADHLCIDEKAPATAELVYNFIRYFKGKLN
                     FKMAQALYTSLLTDTGGFRFNSTRPETHHMAAELLAIGVKPNDIYSQIFEQGRMSSLK
                     LLGRALDRMKSECAEGMHWTYLTKKDFQETGTTKNDTEGFVNYALSLENSMFGVFAYE
                     TDEGFIKFSLRSKGHVDTQAFSKRYGGGGHKNASGISLKEPFDATLKKMIPEMVKYYN
                     ETYGKK"
     gene            complement(15359..16516)
                     /locus_tag="PK754_04330"
     CDS             complement(15359..16516)
                     /locus_tag="PK754_04330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNB08763.1"
                     /translation="MKQKYDVVVIGAGCIGASVASHLGELGCANVLVLEKEKLIGTGS
                     TAKCAGGVRAQFSTPINIYMSHYSIREFETLEKEYGIQFNQCGYLFVVRNEEQKERYL
                     KNMEIQQSYGVDVRFISKDEIAVLAPNYGLDNVLGGTFGAKDGLIDPSMTVDAYFKRA
                     RKHNIDFETETPCTGFKTSGNRITHVITPKGEVACDMVVNAAGPFSKELGKMLGFEIP
                     IEPVRRMITTTGELPFVTNKFPMVVDVTTGMYMHKEGNGLLIGLANKNEKPGYDENID
                     TEFLDEMLTTALEVMPYLENAEIKTQYPGTWGGLYEETPDHHSILSYVPQFSNFIVAG
                     GFSGHGLMHAPAAGRAIAELVTKGTCDTFDLKPLRFTRFAEGDLTKERNVI"
     gene            16873..>17568
                     /gene="sucC"
                     /locus_tag="PK754_04335"
     CDS             16873..>17568
                     /gene="sucC"
                     /locus_tag="PK754_04335"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855522.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HNB08764.1"
                     /translation="MNIHEYQGKAILKKYGVPIQEGIPAATVTEAIDAAKTLKAQGIG
                     AWVVKAQIHAGGRGKGTIYKAKERNEVVQHGGVKFTPDINKVAEYAEKMLGNVLVTHQ
                     TGDQGKLIKKVLVAEGLDIAKELYVGLVLDRARSQYVMMASTEGGVEIEKVAEEHPEK
                     IIKEYVDPTVGFRAFQAQRLGFALGLEGDIHKNFVKFAMALYDAFIGTDSSLFEINPL
                     VITKDNRVVALDAK"
BASE COUNT         4666 a         3804 c         4110 g         4988 t
ORIGIN      
        1 gataagagcc gtaacttccc caaacttcac tttcagcttg ggcagcagtt ttagttaaaa
       61 gggaatttta tgaaatactt agtttggttg agtttactta tctcctttag ccctgtatat
      121 atgggcgatg tgtgcgcgca aggtaaaatt ggttatgtag actttcaata tcttataaca
      181 cagattcagt cggtgcacga tgtgcagacg gagctggaaa aattatcacg tgattggaca
      241 gatcggctgt cggcgatgga agacagtgtg gcattaatag aaaaagatta tgaagcgttg
      301 agtatcacct tgaccaaaag cggcaaaaat gtgttagaga agaatattgc tgatgctaaa
      361 caaaagattt tagtttaccg tgagcagaaa ttttctcctg taacgggtga attatacaaa
      421 aaacaacagg aattactaca gcccatatta gacaaagtga aacgcgccat agacaacgta
      481 cgattgaaag aaaaatatga tgttgttttt gacatttcta caggtaatcc ggtgtctatt
      541 gacaagcgat tagatttaac cttactagtg ttagaagaat taccatccgt tggtttgaca
      601 gtagctcaag tcgaggggca gggaacagga acaaatttaa agacaccagg gagcaatcct
      661 accaaccgta cgccttcggg gcgaagttct aaaagcacaa ccaatgaaac aagtaaacct
      721 gtaaaagaga acgatacaga tcctgcaatc aaagataact aatttacatt tccatattaa
      781 aggagaatat ccgtgaaaaa aacaatgata gcgattctca tgttgacact gggaataaat
      841 acggcggtaa agtcacaagg taaaataggt tatgccgatt ctcagaaaat tttgcaatct
      901 ctgaaagaaa ctgagaccgt tcaaactaaa ttgcaagccg aacaagaacg catttataaa
      961 caatttcaat acatgcagga ttccttgcaa acgatgcagg aagactatgt gaaaaattat
     1021 cgcgacaacc cgctgatcaa agataacatc aaacaaacgt tccaaaaagg tatggaagaa
     1081 ctcgcatacc taatccagaa ttcgcagcaa aaatatcagg aagaaatgta taagaaacag
     1141 caagaactga tggctccgat tttggataaa gtgaaaaaag cgattgaaac agttcgcaaa
     1201 gctaaaggtt atgatatgat tttggacagc tcgccgggta tgattttggc gtatgatcct
     1261 caattggaca tgacccaaaa aacgattgat gaattaattc gtatggccac ggcagctccg
     1321 accacaggaa caggcggaaa ataaaaattt gatttaaatc caaaaaaaat ggaaacttca
     1381 attgaggttt ccatttttta tttattgagg gcggatggcg tcgcgaagtt ataaattttc
     1441 cgacattgct aaattgttag aacggccgtt tgtcggatcg gattctgaaa taaccggatt
     1501 caacgaaccc atgtacgctt tggaatcgga agtgattttt ttgtttcaaa aaaattatgt
     1561 cccccaatcg gtttccggcg cttcaaaatt ttggattgtt tcggaaaaag tttttgctaa
     1621 agatttagct gaacaatgtt tgaaaaaaaa aacggcttat attttatcca aagatcctta
     1681 tgaagatatc ataaaggtac tgacatggtg gattgatcaa agggcgcttc gtagaggtaa
     1741 tgcgtttaaa ataaaaagca aactatataa cggtgtaaaa actcatagat cgtccgtcat
     1801 aggttatgat acgattatag gtcgggggac tttgatttct gctcatgctg tcatcggaaa
     1861 tcatgtgcgt attggaaaag gatgtgtcat ttatcccaat gtcagcatct atgataatac
     1921 gatcataggc gaccgagtga tcattcatgc cggaagtgtg atcggcagcg atgggtttgg
     1981 ttttcataaa acggaagaag gatatttgaa aattccgcat attggacgtg ttgtggtcgg
     2041 tgatgatgtt gagatcgggg ctaactgttg tatagatcgc ggaaccgtcg gtgaaacaaa
     2101 aattggacgt ggatgcaaat tggataatct tatccagatc ggacataatg tgattatcgg
     2161 gccgcatacg gtgatcgctg ctcaagccgg cgtcgcaggt agtacgcgta tcggcgatca
     2221 tgtaactatc gccggacagg ccggacttgt tggccacatt aatatcggcg atcacgctgt
     2281 aatcgctgct caggccggtg taattggttc agtggacgaa aatacaaccg tgtccggata
     2341 tcctgcacgc gaacatcgtg cggcgttgcg gcgcgatgct actctaaaaa aaataataga
     2401 acaatacgat caaaaagata aagtgtaaag cgtatgcaag acattaccga tgacggatta
     2461 gcgagaccgc gtaaagtacc ggaagacctg gatttggata tttctctccg tccgtcccgc
     2521 tttgatgaat ttgtggggca gcagcgaatt atcaataatc ttaaagtgtt tatcgaagcg
     2581 gcaagattgc gcggcgaagc cttggatcat gttttattat ccgggccgcc gggccttgga
     2641 aaaacgacgt tatctcatat cgtggcgcac gaattaggtg tgcatatcaa aataacctcc
     2701 ggtccggtct tagacaaagc cggtgatctt gcgggcattc tgaccaatct ggaagaacgt
     2761 gatgtgcttt ttattgatga aattcatcgt ctcaatccga tcgtcgaaga atatttatat
     2821 tcggccatgg aagattttca gctcgacatt gtcattgata aaggcccgag tgcgcgtacc
     2881 attcagttga cgttgccgca ttttacattg atcggtgcga cgacgcgggc aggacttatc
     2941 accgcaccat tacgttcgcg tttcggtatt acctcgcggt tggattatta caatgataaa
     3001 gatttggcgc acatcgtcac ccgttccgct gaaattttac aggtggacat agacgccagc
     3061 ggtgctaatg aaatagcaag ccgttcgcgc ggtacgccgc gaattgccaa tcggctttta
     3121 aaacgtgcgc gggattttgc gcaagtgcgc ggtaaaaaaa tcattgataa agaaatcgcc
     3181 gattatgctt taaaagcact tgaagtggac gagcatggtt tggacgaaat ggataaacgc
     3241 attttatcta atattatcga acgctttaat ggcggaccgg tgggtattga taatatcgcc
     3301 atagccgtcg gcgaagaaag cggaaccatc gaagaagttt acgaaccgtt tcttattcaa
     3361 aaaggtttcc taaagcgtac accgcgtggt agagaagtta ccaaattggc ctacgatcac
     3421 ctgcgtattc gacctagaaa aacttctcag caggccggtt tagatttgga agaatgatca
     3481 ccggaaaatc taattgactg tcaagagatc cttgagtgcg cgggttgttt cacgggggtt
     3541 tacggataga ccgatcgcag atattacggc aatcccatca actccgcatg cgagaacttc
     3601 tttaacatta ttttggttta taccgccgat cgctactaca ggtaacttta ccaaatcccg
     3661 aatttgtttt aaccccgttg taccgataat gccgactata tcttttttgg atgccgtcgg
     3721 gaaaacggtg ccgacgccga tataatccgc gcccgcggat tctgccgttt gcgcttcctg
     3781 ttgcgtgtgg gttgaaattc caataattgc cgaatcgcct aatatcttac gcgctgctcc
     3841 gacggtaata tcctcttgcc cgagatgtat accttccgca ccgatagccg tggcaacttc
     3901 tatacgatca ttgatgatca gcggaatatt tttgctatat gttacttggt gaattgttcg
     3961 ccctagcgca atcatcgcat tgacagaaag agtttttgct cgcagttgta cgaacgtgac
     4021 accgccttga atagcttctt cgactacaga acatattttg ccggcgggta tcatgccgac
     4081 atcagcaata aaatataagc ttacgtttaa tcgtttattc ataacacatc acaataaaaa
     4141 actcccgaca ggtaatgccg ggagtttttt tgaaacataa caaatgttaa atagtctggt
     4201 tttcgtcgaa attttcgaga tggtatgcga cgcgttgcag atactgtccg ccgagagcgc
     4261 cgtccacgac acggtggtcg taagagagcg atgcatacat gattgggcgt attacgatca
     4321 tatcttcacc atcatcggta gtgatcacta ctgggcgttt tttgatcgta ccaacgccca
     4381 taattgctac ctgaggttga ttgatgatag ggaaacctac cgtcgtaccg aaaacgccca
     4441 tattggtcaa cgtaatcgtt ccatcctgaa tatcttcggg gcgaagcttt ttagagcgtg
     4501 cgcgcaaagc gagatcattc gttgcgcggc aaacgcctac taagttcatc gcatcggcat
     4561 ctttgatcac cggtacgatc agcgccgtat tattttcgat agataccgcc atacccacat
     4621 tggtgtagtt tttcttcacg atggtattac cgtcaacgga gatattgatc atcgggaaat
     4681 ctttgatcgc gcgcaccatc gcatcaatga gaaacggggt aaaagtaagt ttgaatcctt
     4741 cgcgtttttc aaaagccgat ttgtatttat cgcgaaaacg tacgacgcgg ctcatatcaa
     4801 tttccgtcat cgaatacacg tgtgcggaag tgtcaatact ccggcgcatg tgttcagcgg
     4861 taatgcggcg tatattattc atttcgatga gttccgagcg ttctttattg tatccgtatt
     4921 taacgtcgga aacttgtttc gccggtgcag ccgctgtaga tgcggatgtt gtcgtcgtga
     4981 cagcaggacg aacgccgcct ttgggacgtt tagctaaata ggcgagaacg tcatttttgc
     5041 ttacgcgatg attaagtccg gttccggcga tcgaatccag ttcctgcatc gaaagccctt
     5101 cagctttagc gatggaacgt acgagcggcg aataaaaacg attgctctcg cctttgacca
     5161 tgccgccgcc ggcatttaca gtagctgcgg gagtcgccgc tgtcgctgcg atttggttct
     5221 gtttctggaa gtcctcaata gaactagaaa aatgcatttt ttcttcggtt ttgggcgtcg
     5281 gtgcggccgt tgttgaagcg gcgaccgtag cgccttcggc accaatacgt gcgatcaaag
     5341 ttccaacggc cacggttgta ccttcaggaa cgataatttc agtaatcacg ccggatgccg
     5401 gagaaggaat ttcagaatct actttatcgg tactgatttc gaggacagtt tcatctttgg
     5461 ccactttgtc gccggctttt ttatgccatt tcaaaatttt accttcggca atactctcac
     5521 ccatttgagg catgaccata tctaccgagg ctccaccgga agcgggcttt gaagcaggcg
     5581 tgttttgtgc tacaggagct gttttggact caaccttagg agaaggtgta ggtgccggcg
     5641 atgcgaccga ggcgttagca tcggtttcga tacgagcgat caatgcgcca acagcgacgg
     5701 tcgtaccttc aggaacgatg agctcagcaa taatgcccga tgcgggcgaa gggatttccg
     5761 agtccacttt gtcggtgctg atttcaagga cggtttcgtc tttgcttatc ttatcgccga
     5821 cttttttgtg ccatttcaga attttgcctt cggcaatact ctcgcccatc tggggcatga
     5881 ccatatccac ttttgccatg agtaacgctc cttaattgat catgtatgta atatagtata
     5941 ttcttcgaat taagcggggc aaagtatcaa aaaagagttt cacattcaat accaatttcc
     6001 cttgtcagcg atgcttccat tccggttttc gcttttgcat aaaagcggta ataccttctt
     6061 cgatatcgtg cgttttgacc agctcttgga ggtatatgtt ttcagccgta tcaagtgcgg
     6121 tagtgaaggg tttatccgcg ccggccagca atgctcgttt ggtttgttta agtacggtac
     6181 cgctgagggc attcagattt tgtaaaagcg tatcggtagc agatcgtagt tcatcgggcg
     6241 gcaccacctt gttgatcaaa ccgagcgtta aggcctcgtg cgccttgatg ttttcaccca
     6301 gcatgcaaat ttccatagcg cgacgtaacc ctattttttg tggtaaccat gccgcagcaa
     6361 ccggagggta gcatcctact ttgatttcag gttggccaaa aatagtcgaa tcggatgcaa
     6421 ttacgaaatc acagaaagta gccaattcca taccgcctcc gaggcaatag ccgtggacag
     6481 aagcaacggt gattttatcg gaattagcca aagtgcgaaa aatatgatga aacgatttca
     6541 acatactgtt gatcttgtcc aacgtgtgat ccttgatatc aacgccggtt gaaaacgctt
     6601 tatcacctgc accgcgaaac acaatcgttg ttacatccgg attgcttgtg attttatcta
     6661 atgcaacgtt tagttcatca atcatggaca gattgatgac attgaccggt gggcgattga
     6721 gcgtcacgtg cgcttcgcgc gcatgatgtt cgacacgtat cgttgtataa tccatagata
     6781 ctccgtgatt attcgttgta taaaacttcg actaatacat cgccgatttg ttgcaggctc
     6841 tcggcactgc atttatcggg tgtatcatcc atcgtgtggt gataacggtt gctgtcatcc
     6901 ggataatcaa aatctatgat gtcaatacat ttgatgccga tccgaagcag cggtatatgg
     6961 tcgtcataaa cattgctttc aatcgtttta cgaaaaaccg gtttttcaag gcgtttggcg
     7021 gcatcccaga ctttattcac aatgtccttt gcaccggtgt atgaattttg ctcaatcggt
     7081 aaatccagat ttttatcacc gatcatatca agcagaatgg caaattccgg aaaatatgtt
     7141 ttattattct ttgcaaagta ttccgatccg atacaataag cattgagact atccggtttg
     7201 gattcccaat tgtgatcgcc gtaatcttcc aaatcaaaaa gaataatgtc cacaccgatg
     7261 tccagcggct gttgctttat ggtacgcgcc atttcaagta ataccgctac accggacgct
     7321 ccgtcattgg cgccgatgat gggcttcatg cgattggccg gatcgggatc tttatcggcc
     7381 catgggcgtg tgtcccaatg cgcacaaaga agaacgcgct ttttattttt aggattgaga
     7441 ttgatggacg cgatgatatt ggtgagttca aattttttgg ttgtatcgcg tttgtccgtg
     7501 taatgaaatt tctgatgatc tacgcgatcg caatatttac gaagctcttc cgtcatatat
     7561 tcgagcgctt tttggtggcc ttcagatccc gggtttcgcg ggccgatttc gcattgtttt
     7621 ttcaggtaat tcatggccga ttggccgtca aattgcggct ttacaacaaa ccaatggcag
     7681 ctgttgaccg atatcatcag accggtcaaa atcagaagga atagactgcg catgcaaact
     7741 ccgtgttaag actgttgttc gggcgatgca tgatgcttgg ccccgttatc agatagtacc
     7801 gatgaaacgc tttgggattc gatcacggca taagcgtcat gattatgaat actttctaaa
     7861 ttagtcacat ctacacggta acgcacgata cgagcatctt gcgagagttt gatggcgatg
     7921 tcgcgtacca ggtcttcgac aaatgccgga tgatcgtatg cgcgttcggt tacgtatttt
     7981 tcgtcttcgc gtttgagtac gacatagagc tcgctgctgg cacatgtttc aacggaataa
     8041 accaattctt cgatactgag aaaattatcc gtttcacact cggcttttac ggttacaagg
     8101 ctgcgttggt tgtgtgcacc gtattcggaa atagacttag agcaagggca caacgtggtg
     8161 accggcacct gtacttccaa ggacaacgtc attttatcgt gaatgctaga cgcgtcgatc
     8221 gcaacggtat aatccataaa acttttggtg agtgtgacgg gcgcttcttt ttcgataaaa
     8281 tatgcaaaag tcactcttgc aaaagcgcga tccgcgtgca accgttcgcg catcatattc
     8341 ataaaataag gcatgctttc gatggataat ccgtcctgcc acgcattgag gatttcgccg
     8401 aagcggctca tgtgcgtgcc ttttttatcg tggggcaaat caacagccaa accccacatg
     8461 gcaacggttg gagtgcgtcg gcctgcggca ttgataaaag tcagaggata tttgaggttt
     8521 tttatgccga cccgttgaat gggcatttta cgaaaatcgt ctcgaccttg cgtatcttcg
     8581 atagcggaca ttgttttcct tatgttatag actaaccaaa tacctcggcg gggataccga
     8641 gcgtacggac ttgagcagct attttttcta attcggttgc gcttattttt tcgagtgttt
     8701 taaccggtgt atcgcgatca atcggatata ccattacttt tcgcggtttt atagcttcga
     8761 tgcgacgaag ccatgcgtga acttcttccg gcgtagtatt atcaatcacc gtgttttggt
     8821 gttcgccgcg taaaaatagg gtctgaatga ttaggttgcc attaaatttt ttaagagttt
     8881 ccgttatacc gtccagcgaa attcctttat ccggtatatc aatccgctga aacatagttt
     8941 ctgtcccggc atccaatttg agaatggggt cgtcggtttt tttgagagcg tcgaatattt
     9001 cagttttgtg aacacgcgtt gcattggtta aaacagcaat acgggcattc ggaaagtatt
     9061 tctcacgttt ttcacgggtg gtgtcaataa tggctgaaaa attcgggtgc aaagtcggtt
     9121 cgccgttgcc ggcaaaagta atgtggtcaa tgttctgatt ttgttgccgt aatgccgata
     9181 gtttggactc gagtgcctcg gtaaacgcct ccaacgtcgg gaccgcatct ttgacggacg
     9241 tatcggtcca tccgcattcg cagtacacac attcaaaatt acacacttta cgcccaaccg
     9301 gaagcagatt gatccccagc gatgtaccca atcggcgcgt ggtcagcggt ccatatattg
     9361 tagtggcgtg taaaaacatg cgtatcagct aaaagtggat aatttgaaat tcttaggaac
     9421 cgtacatttc cgtaatatcc tcattttttt gcagcacacg aaattcgtcc atacggattt
     9481 cctgttcgcc ggtgacacgg ctaaaaatca aataacgcca acgcatattt ttgattttgg
     9541 gcttgacctg ctccaaaata tcggaattaa caacgaattc gagtttggag aaaaagctgt
     9601 ttttttcttt gtaacggcgc gcccaacgtt cgattttatc aaatagcgtg gtacgattgc
     9661 ccacgcgacc cgtaccgcta caggccggac aaggttcgct gagcgaaaac ataatactcg
     9721 ggcggatgcg ctcgcgcgtc atctcaataa gaccgtattc gtccatagga agaatgctga
     9781 atttggcgcg gtcttttcga agttcttttt tcatttcttc aaaaagtttc ttcttgttgc
     9841 gctcgtcgtg caaatctata aaatcaataa caataagtcc gccgatatcc cgtaaacgca
     9901 actgcctgca ggtctcacgc gcagcgacca tattgacggc catggagttt tcttcataag
     9961 aaccgcgtcc gatgtatttg ccgctgttga catccaccga aatgagcgct tccgtatgtt
    10021 cgatgatgat gtaaccgccg tttttgagcg gtactttttt ctcgatactt tgttgcagat
    10081 cttgttcgat attatacaaa tcaaatattg gcttgttact gcgatagagt tcgatgcgat
    10141 taccaagttg cggggatgtc gtcgagatat agttagaaat cgatttgtat aattttttat
    10201 catcaataag tacgcgggag acatcgttgg agaaaagatc gcggatgacg ctggagacca
    10261 tttccatatc tttatacacg agattgggtg cggatatttt ttgagctttg gtatcaatat
    10321 cgtgccaagt gtccaaacaa ttacgaatat cgttacgcag tacttcttca tcgcgctctt
    10381 ccgcattggt gcgtacgatc agaccaaaat cccgcggttt gacttctttg gccaatcggc
    10441 ggagtcgttt tctttcggga acagtattga tcttccggct gataccgatc atttcattgg
    10501 gatcgttggg gatgagcacg gtaaatcggc cgggtatcga aatctccgaa gttacacggg
    10561 cgcctttgtt gtaaatcggc tctttgatga cctggacgag aatttcctga ccgacgcgta
    10621 aaaagcgtcc gtggttatca tgattatgaa tacgggggtg attaaaaggt ttgaccggcg
    10681 cttcgttatc gtccgcgtca tcggcatcat tgatgacggc atattcgtgc gtgttatcac
    10741 cgatatcgga aaaatgcaga aaggcatctt gcttcatgcc gatattaaca aaggcggctt
    10801 tcatgccggg gaaaactttg gcgacgcgac ctttgtaaat gtcgccgacc attcgatttt
    10861 tttccggccg ttcgacaaaa atttcgacga gccgttcatc ctccacgatg gcgatgcgcg
    10921 tttccgcatc ggtcgcgttg atgataattt cttttttcat acgtgtccta ttctatcgaa
    10981 tagggcagag gttcttcctt aacgtacagg atcggatttc caaaactatt ggaaaagaac
    11041 aaatatataa atcacggatt tgcaccgtgg tattcgcggg cagaccgata tcagataaca
    11101 tcgctgcgcc cctaaacctg gaagaaaatt tctgccacat tcgtggttac aattttatat
    11161 atatcaaatg ataatacatt catacgttgc gggcgataat acgccaaagt ctctaaaaaa
    11221 gcaaggtttt gctttatggt tgatgctccg gtcttaatag gtcattgatc gttttgacgg
    11281 ggttgaacgt aatgagcggc acctcggcaa aaatagtaat ccacttagcc atggccccat
    11341 tccataaccc gggtaactcc atagccttta tttcacgtcc atctttggac tttttggaaa
    11401 taaagccggt atggggatca ataaaacgat gcaggtcaaa tggctttcct tcaaaatcgc
    11461 ggacgccgca aacgatatct accgggttaa aatgtgtaga gtcatctaaa atgagttttt
    11521 gtttgggcca ttgaagatca atctgggctg tttccacgat ctggagcgag cggtttccgt
    11581 taggttctct aacccaaaac ggaccacctc cgggttcgcc ttgattacgc accattccgc
    11641 aaacacggat cgggcggttt aaaaattgca tcaaattatc acgccgctcg cggatatcca
    11701 tcgtcgaact tgcttgcatg ccaagtcgtg aaatggcaaa cgattcggct tctgtgaccg
    11761 tatcggaatt cggaggatta ttttttagtt ttttaagata accaaaaatt tctagctgca
    11821 tttcaatcag atgaccgcat aaaatttttt tgtatcggaa tgtcggctct ttgagacgat
    11881 caggtacgac attatcaatg tttttgataa aaacaatatc gcctgccaga tcgttaaggt
    11941 tctcgatcaa agccccatgg ccggccggtc gcagatgtaa acgaccatcc tgatcgcgga
    12001 caggcctatt gtgcaaatca acagcaaggg tgtcggtgga aggtttttgc tctgaaattt
    12061 caatttgaat gcgacggccg ctcgtttcgt aacgagaacg caccgtttga atgtgcgaat
    12121 caataagttg gcgatattcg cttgaaatgg taaaatgcac gtacgcattc tgatccgtat
    12181 ctgtcgcata agccagagct tcggtcaaat gttcttccaa agccgtacga gcaccttcgg
    12241 gataacgatg aaaaggaatg agcgccttgg gtcggtgggc atagtccaaa ccgacatcat
    12301 gcatcagata tgataatacg tccgaatagg aacgtgtttg gcgcaaggtt tccaaagaca
    12361 aaccgtcctt tgccaaaaca ccgtctaact catcatagaa agcaaactgg gtaagccgat
    12421 caaaaaatgt ttttaaaaaa cgcgtgtcgg ggtgagaggt attttgttga agcatacgat
    12481 cataatgaac ggccatcaaa cttttgaaca tgcgtgtagc cgcgccggaa gcaggaacaa
    12541 atttttttac acgccctgcc gccgcggcat cgtcggcttt ttttattaag gcatcgtgcg
    12601 tactgtcgga taaaactgtt atgccgtcgc ctatggtgca gggacgatcc aattcgacaa
    12661 acggaaatcc ggttttgaaa aattcgattt cggccgatag ttgttcgggt gacttgccga
    12721 tttgagcaag taaagcgagg tcattatccg tgagtacgat atccgacatg atcaccagtg
    12781 gcgcaacatt tcgacagtaa gccttacgcc aaatcctgtc gcagaaatgc gatccgtgta
    12841 aggaagttct tcttcactat tggcggtgcc ggcgatatcc aaatgggccc acggcgtatt
    12901 accgacaaaa tgtttgagaa atgccgccgc ggtggaggcg ccgcccgggc gaccaccggt
    12961 atttttgatg tcggctatat ggcttttgat ttgtttgtca aattctttcc acagcgggag
    13021 ttcccatatt ttttcgccgc ttgcatcgga tgcgtgcatc aacttttgtt tgatattact
    13081 atctgtggca aaaagaccgg cgccatgcat acccaatgca atcacacaat gtcctgtgag
    13141 cgtagcgtag tcaatgatca cttccggttt gtagcgctga gcgtaagcca gcgcatcggc
    13201 gaggatcaat cgtccttcgg catccgtatt gatgatttca accgttttgc cggaatacat
    13261 agtcaaaaca tcaccgggtt tataagcgcg gctgccggta agattttcag aagaggggac
    13321 gatgcctacg acatacaacg gaagtttcaa ttcggcaatt gcttgcataa taccaatgac
    13381 ggctgcaccg ccgcacatat caaacttcat gcgatccatt tggtccgccg gtttgatcga
    13441 aatgccgccc gaatcgaatg ttaatccttt gccgacgagt acgatcggtt tagtgttttt
    13501 gggcttagcg ggtttgtatt ccatggtgac cagccgcgga ggttcggcgc tgccggcggt
    13561 gacaccaaga atgccgccca ttttaagcga tttgattttg gtctcattga atacggtaca
    13621 tttgattttg tgcgtacgac acatcgccgt tgcacggtcg gctaattcac gcggaggcaa
    13681 ttcattgccc ggcgcattgc ctagatcacg ggtaaaattg accgcattgc ttatgatttc
    13741 tccggcccga acaccctgtt gcagggcggc tgtcggctca tgatcggcaa taagcgttag
    13801 ctctgtaatg cgatgccggt cttgcggctt tttgtccgta tggtacttat catacttata
    13861 atgcgcaagg caagcgcctt cggtaagtgc ttgagctatg cgatcatcgg tccagttttt
    13921 ttgccgcaaa tgcgcaggaa aggcgagcgt gattttagaa tattccatag cgagtgccgt
    13981 acgcacggct ttggctgccg ttcgccgcag agtttccggc tcgagatctt tgtattcgcc
    14041 taacccgata agtgcaatgc gtttacttga ggttttgtca ttacccaaaa ggagaaatac
    14101 tttttcttct tccgcgccaa attctttgga cttcagtaac ttgggaatcg tgtcttcgta
    14161 gatatcttca agttcatttt tccaaaaatt tacaggttcg tttttggcaa taaacacgca
    14221 cgttaattcg cttttccagt gcttgagctt ggaatatttg gccgatacgt gcataattat
    14281 tttttaccgt aggtttcgtt gtaatatttg accatttcgg gaatcatttt ttttagcgtg
    14341 gcatcaaatg gttctttgag cgaaatgccc gacgcatttt tgtgaccgcc gccgccgtaa
    14401 cgtttggaaa aagcttgggt gtcaacatga cctttggaac gcaggctgaa cttgataaat
    14461 ccttcatcgg tttcgtaagc aaatacacca aacatcgaat tctcaagact cagagcgtag
    14521 ttaacaaatc cttcggtatc atttttcgtc gtaccggttt cctgaaagtc tttctttgtg
    14581 agataagtcc aatgcatgcc ttcggcgcat tcacttttca tgcgatcaag agcccgcccg
    14641 agcagtttca gggaactcat gcggccttgt tcgaaaattt gagaataaat atcgttgggt
    14701 ttcacaccga tagcaaggag ctcggcggcc atgtgatgcg tttctgggcg cgtagaatta
    14761 aaacgaaatc cgccggtgtc cgtgagcaga gaagtgtaca gcgcttgggc cattttaaag
    14821 ttgagcttac ctttgaagta acgaatgaaa ttatatacca gttctgctgt tgccggcgct
    14881 ttttcatcaa tgcacaggtg gtcggcaaaa ccatcgttat ccaaatggtg atcaatacat
    14941 atcgtcgtac ccggcgcctg gacgactgcc tcttgcattc gatcaagtcg tttggaaacg
    15001 ctgatatcaa gcaccattac gacgtcggct ttttgtatga ccttgtcgtg tttggcaggt
    15061 tcataagttt ctatgatgtt gccataattc aaatactgaa aagctttggg gattgggctt
    15121 gtattgatta tacgcggtga ttttttgagt gatttcagga aaagatacat tgccgcttcg
    15181 ctgccgagac catccccgtc agggttgacg tgggtcgtca aaacgaacgt tttcgctttg
    15241 gctatcaccg gttttatcgc gtcaaacata tttttctttt tggctttttt cgttgccata
    15301 acgccatcct cagattggtg tcgatatagt ggattatttt aagagaaccg tttttctttt
    15361 aaatgacatt tcgttctttg gtcaggtcgc cttcggcaaa acgcgtgaaa cgcagtggct
    15421 tcagatcaaa cgtgtcgcat gtgcctttgg taacaagttc tgcgatggcg cgcccggcgg
    15481 ccggtgcatg catgaggccg tgaccgctaa agccgccggc cacaataaaa ttactaaact
    15541 gcggaacata actgagaatg ctatgatgat ccggtgtttc ttcatacaaa ccgccccacg
    15601 ttccgggata ttgtgttttg atttctgcat tttcgagata aggcatgact tccaaagcgg
    15661 tcgtcaacat ctcgtctaaa aattccgtat caatgttttc gtcgtatccc ggtttttcgt
    15721 ttttattagc gagaccgatt aaaagtccat taccttcttt gtgcatatac ataccggtcg
    15781 tgacgtccac aaccatcgga aatttattgg tcacaaaagg taattcaccg gtggtggtga
    15841 tcatgcggcg tacgggttcg atgggaattt caaaaccgag catttttccg agttctttgg
    15901 aaaaaggccc cgctgcattg accaccatgt cgcatgccac ttcacctttg ggtgtgatga
    15961 cgtgcgtgat ccgattacct gaggttttaa atccggtgca aggtgtttcg gtctcaaaat
    16021 caatgttatg tttacgcgcg cgtttgaaat aagcatcgac cgtcatcgaa ggatcaatca
    16081 agccgtcttt ggcgccaaat gtacctccga gcacgttgtc caacccataa ttaggtgcaa
    16141 gcactgcgat ttcatctttg gaaataaacc gtacatctac gccgtaactt tgttgaattt
    16201 ccatattttt caaataacgt tccttttgct cttcatttcg aacaacaaac agataaccgc
    16261 attgattgaa ctggataccg tattcttttt ccaatgtttc aaattcgcga atgctgtagt
    16321 ggctcatgta aatattgatc ggcgttgaaa actgagcgcg aacaccacct gcacatttgg
    16381 ctgttgagcc cgtaccgata agtttttctt tttctaaaac caaaacatta gcacaaccga
    16441 gttctccgag gtggctcgcc acgctggcac cgatacatcc cgctccgatt accaccacat
    16501 catatttctg cttcatggga atccctgtaa aatataaacg taataagtta ggattaaaaa
    16561 ttcataagct taatataaat caatatttag tgaaaggcaa attgaaagtt tgggttgaga
    16621 ctatgatgat agttgtcatc tgatgttaac aagtacaatt taaaccgagt ttattgcaag
    16681 tataagatta ataataagat gtattgtttt agttaagact cataaagtga catgatgtga
    16741 aaatgaaatg attagtattt tgaatatttg gtataaagca aaaattcttt ttgattatac
    16801 aagccgattt tatatattgc gcgcaattca aaagcaaaaa actattccac gttcatcgga
    16861 ggatatgatt acatgaatat tcatgagtac caaggtaaag ccatattaaa gaaatacgga
    16921 gtaccgattc aagagggaat tcctgctgct accgtaacgg aagcgataga tgctgcgaaa
    16981 acattgaaag cgcaaggtat cggagcatgg gtcgtcaaag cgcagatcca cgccggcggt
    17041 cgcggtaaag gtactattta taaagccaaa gagcgcaacg aagtggtgca gcacggcggt
    17101 gtaaaattca caccggatat taataaagtt gcagaatatg ccgaaaaaat gttaggtaat
    17161 gtgctcgtca cgcatcagac cggcgatcag ggaaaactga tcaaaaaagt tttggttgcc
    17221 gaaggtctag atattgccaa agaactctat gtcggcttgg tgttggatcg tgcacgctcc
    17281 caatatgtga tgatggcgtc tactgaaggc ggcgtcgaaa tcgaaaaagt ggcggaggag
    17341 catcccgaaa aaatcatcaa agaatacgtt gatccgacgg tcggctttcg tgcgtttcag
    17401 gcgcagcgcc tcggatttgc cctcgggctc gaaggcgata ttcacaaaaa ttttgtgaag
    17461 tttgccatgg cgctgtacga tgcgtttatc ggaacggatt cgtcactgtt tgaaatcaac
    17521 ccgctggtca ttaccaaaga taaccgcgtc gtggcgcttg atgccaaa
//
LOCUS       DAOQNQ010000039        17369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1158_length_17369_cov_5.208675, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000039 DAOQNQ010000000
VERSION     DAOQNQ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17369
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1158_length_17369_cov_5.208675"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="PK754_04340"
     CDS             complement(<1..872)
                     /locus_tag="PK754_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08765.1"
                     /translation="MKLLLLIGIGLNLSSTFSIVALSQTHAFTNVHVIPMDSFRILHH
                     QTVIITDGKIAAIGNTREVSIPKEASIIDGKGQYLIPGLIDCYAHIHEKNLMLFLANG
                     ITTVRNVPGAAFQHAIKKLSDTGKLQGPRIYSTGPAVTGSVVAYHTQGMLLNADEARF
                     AVRDVKRMGYQSVFSYVTITPDIYSAVLDEARQQGLPVQGHVPYMIPFEEYAEGSQLS
                     FDNLVGLLNPRSGDTYPKEQLIQFAEAFRKNGKYVIPTLTIHKARSLAHKSDSLKLRA
                     EMDYVPPRQRVYWHL"
     gene            complement(904..1530)
                     /locus_tag="PK754_04345"
     CDS             complement(904..1530)
                     /locus_tag="PK754_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNB08766.1"
                     /translation="MHVLIMITCISLGSFGFGSCQDGEATDTTVVVADSLDFVLPFPV
                     GKRYKVSQSNNTIWSHSPNNFNGVFRYAYDFDMPVGTVLTASREGEVVFISDNFEDSD
                     HTLGHENVLVIRHRDATYSRYIHIRKHGALVKTGATVAKGDTVALSGNSGYTLGIPHL
                     HFDVVTAPTGLPDINAQSIRTLFKNSTAYPNALQQNERYRALPYTFSK"
     gene            complement(1542..1826)
                     /locus_tag="PK754_04350"
     CDS             complement(1542..1826)
                     /locus_tag="PK754_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020531968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08767.1"
                     /translation="MKKVSTILTRHPQGKQGVRIDKSKYDMIRGFILSTLKERGPMPF
                     EELADYAVKILSSDFEGSVVWYIVTVKLDLEARKLIIRLPRKSPQWISLS"
     gene            2195..4855
                     /gene="fdhF"
                     /locus_tag="PK754_04355"
     CDS             2195..4855
                     /gene="fdhF"
                     /locus_tag="PK754_04355"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HNB08768.1"
                     /translation="MIKFTINGRTHEVSEGTRILEAASSVGIQIPTLCHDPRLNPSGV
                     CRLCLVEIEGSSKPVPACRTEITSDMHIETHSAHIEKYRTDLLKMLHARYPQEVVEAF
                     PEKELHRLFQHYVPRFEEPVTVKSSWKDTTHPYIRIDMSLCVDCHRCERICSEVQGQF
                     VWNPIQRGFATTMTPEGRPTLLESGCVSCGACADTCPSGALIDKNVFMLGEPTSWTKT
                     TCPYCGVGCEMMVGTRDQKIVTVKPSMDGPVNKGHLCVKGRYSHGFVGSGDRITEPMI
                     RRDGAWQKVSWDEAYAFTASELKRISANYGASSVGVIGSARATNEDNYLTQKFTRVVL
                     QTNNVDSCARVCHAPSAKALGNILGTGAATNAFDDIERATTIFICGANATENHPIVGA
                     RIKQAALRGAHLIVMDPRVIELAEYADIHLAVRPGANVALLNAMACTVIEEGLTDDEF
                     ILAHVSGYEKLREFLKDFTPEKIADQCGVDAATIRQAARLYATAKPAMCMHGLGMTEH
                     TQGTDSVSGLINLALITGNMGKPGTGVNPLRGQNNVQGSAHMGCEPTHLTGYGTVDEG
                     RTLFEATWKVSLPTDKGLNLMHMLDAAEKNQFKAMWIVGYDVYMTNPDTDFSRRAFEN
                     MELVVIQDMFMNETARLFGTVFLPCTSSFEKDGTFMNGERRVQRVRKAIEPCGQSKPD
                     WLIICELAHAMGVREGFEFESAEDVWNEIRRVWKAGYGITYPKLEKGGIQWPCPTEDH
                     PGTDILHRTTFPVGARAEVQMLPFSAPHYDARYPWTLITGRSLFHFNSGTMTGRSKNA
                     QIKRPDYVEIAPTDAAQLGVQTGDTLRITSENGQAFVPAIVTDHVKHREVYATFQEPE
                     NLLNAVIGMERDDKTATPNYKVTQVRIEKV"
     gene            4939..5012
                     /locus_tag="PK754_04360"
     tRNA            4939..5012
                     /locus_tag="PK754_04360"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4973..4975,aa:Asp,seq:gtc)
     gene            complement(5033..>6096)
                     /locus_tag="PK754_04365"
     CDS             complement(5033..>6096)
                     /locus_tag="PK754_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08769.1"
                     /translation="YSGFGPTLFLELYNITRSSEFRDDTNPGNDIPYESIQTNSFELR
                     EVDAGMDWSLFAPRDIRINYAHIESFVRIRNKEYDLDAENYLLTFSSGAIKYYYGNDF
                     STTWKFNNVPPAIDKEINPRGGRQMSVSVGYYMDNLLNGFAIEPSSGSNKTLYKRDAY
                     LRNENTWTEFIRLPFGDHTLEARLHTGFIPTKVDSFYNFFGGSLVGLKGYSYYSIEGR
                     YLGLFNAVYRFPLIRRIDKKAGPLYFDKIFGGLTFGMGNAWSTRSTMDRWKRDVGIEL
                     RTEMYSFYVYPTRLTFNAAYGIDRFTGYGPMITKYKSEDGSYYNERQKVKSGGSWRYY
                     LTVLFGFTLFDEAPKMKNP"
     assembly_gap    6097..6196
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6197..8400)
                     /locus_tag="PK754_04370"
     CDS             complement(<6197..8400)
                     /locus_tag="PK754_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08770.1"
                     /translation="MKKTFLVFEILFFVLILVRSGYAQPEAWSHGELQWFTIRSAHFD
                     VHYHDSPYTALDVSRKGPERTAREAAAIAESIYETITYLYDHRPERIHIIIRDTDDYS
                     NGGAYYYDNKIEIWASNLNFELRGTHHWLRNVITHEFTHMISIQAMMKLSRRFPGIYL
                     QYIGYEKERRQDVLRGFPNRIVSYPVAGVMLPVWFAEGVAQYQQKDLDYEFWDAHRDM
                     ILRDRVLGEQMLTLNQMGTFGKNSIGNESAYNSGYALVSYIAAHYGDSSLKKITHYSS
                     KLFYSFERAVKVATGDDIDTIYRQWKNLITEQYALHTTDIRAHARIGREIATEGTGNF
                     YPALSPNGQYLAMISNRGFDYLSQASLYIVDPSNSNIIHEIKKAEGPLSWSPDSRYIA
                     YAKNTDDNDYHSQYNDIYVYDMVRRKEFRITCGLRAFAPAFSPDGSSLAAIINGDGTN
                     NIIRIVGFAEDVLNEKSTFSKNMFVRDGMHWRRITHFDDGRQIYGLCYTDNGRTLIFD
                     TSTDDGRDIFSVDTSGNHWKPVRQTRKDERSPVVSQDGQTLYFSADETGIFNLYAMNL
                     HSGDIRQITNVTGGAFYPAVGQNNILYYTLYQNTGFRVYSIADTTGFDSKQTAYGLEN
                     PNILSKPNDKPYPGKWPSEHRIFTVIPPRTDTAALYKNTFLKFSFLPVLRLDYGTFKP
                     GVYFYSSDVLSKSSFFGGAMFNIPDMERDLFGIFEYSGFGPTLFLELYNITRS"
     gene            complement(8397..9587)
                     /locus_tag="PK754_04375"
     CDS             complement(8397..9587)
                     /locus_tag="PK754_04375"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HNB08771.1"
                     /translation="MTRKRIIFFSGKGGVGKTTIAAATALRCADRGTKTILLSTDPAH
                     SIGDLLGLPIHATPTPVAPNLDAVHIDPHAELDSNWGHIRDYFSNLIQTLGADGAVSG
                     ELAILPGMDNLFSLLRIRDFHDKSDYKVIIVDMAPTGESLRLLSLPQIVSLALKITRV
                     IEKYIVSPVIRPASRMSKSLRVVVAPEDVARSWEAILEKILAMRDLFEKESRTTTRLV
                     MQPESMIMNETQRALTYLNLFGMVVDQVIINRILPDETQKGYFRIWHETQKKYIHAIT
                     RDFTPIPIAHAPFFHHEIHGLNDLRTLGNALYNQADPASVFYNSRPVQFVNTKKEKLM
                     KLKAPFLDVQNTPVSLHRRGNEIMIEIGSFLRTHTIPDSMAALEPEGADYNNGELIIR
                     FIKK"
     gene            complement(9597..10118)
                     /locus_tag="PK754_04380"
     CDS             complement(9597..10118)
                     /locus_tag="PK754_04380"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNB08772.1"
                     /translation="MKSIIKSLYTIILILSPFGIIGCSSVPKTVSHAKTVKDEMMLIG
                     NITREELFQEMPVFQKNYDVYNPAGSAIETLHRMSTPTDILIVLGTWCGDSKHHVPSF
                     LKTLDAAKNPNLTYRLLAVDRTKKDSLGVTAQYNIKRVPTMIVLRNGAEIGRMVEFPQ
                     KTVEEDLLDIVKP"
     gene            complement(10102..10911)
                     /gene="tatC"
                     /locus_tag="PK754_04385"
     CDS             complement(10102..10911)
                     /gene="tatC"
                     /locus_tag="PK754_04385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNB08773.1"
                     /translation="MADEEKRALPGSTGGDMTFLDHLGELRDRLIKIVLAVLLMMAIS
                     FVYSNTIIKWLLYPPMIIPDLKPPIQLMRPVITQVQGLMMVKIQVGLIAGIVLSIPVI
                     AYQLWRFISPALKKSEKRFAIPLIFFITFFFLLGATFAYLVVIPITLNFLLSMGNIDT
                     ELGIVVENMIDVDQYLSFVSGFMLISGATFEMPVIAFFLAKIGILSPQFMREYWRHAA
                     VACLLVSAVITPTTDMITMFVVAAPMLVLYEMSIWVAKFATRKKKSDEKHN"
     gene            complement(10918..11352)
                     /locus_tag="PK754_04390"
     CDS             complement(10918..11352)
                     /locus_tag="PK754_04390"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HNB08774.1"
                     /translation="MKEIIRDIRFEIQHMDRSPASARRFGFILTTILLLIAGWLWYKE
                     RDVWMYWAAPGVLVGLISAIAPNLIYPLYTGATIFSIFIGYFVSRVILLIMFTLFFVP
                     VGLITRLFGRDLLKKQIDPKTNTYWTKKESPVKTPEHYERMF"
     gene            complement(11366..13186)
                     /locus_tag="PK754_04395"
     CDS             complement(11366..13186)
                     /locus_tag="PK754_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HNB08775.1"
                     /translation="MNILGISCFYHDSAAALVRDGKIIAAAQEERFTRKKHDDNFPKH
                     AVEFCLKTAGIDMSQIDVVAFYEKPLVKFERLLRTYFAYAPIGFNSFIKAMPQWIRKK
                     LWMQELLMEELPGFKGKIIYPEHHESHAAAAFFPSPFQDAAFLTIDGVGEWTTTSFGT
                     GRGNKLQIDYEIHFPHSLGLLYSAFTYYTGFKVNSGEYKVMGLAPYGEPKYVDLIYKH
                     LIDLKEDGSFRMNMDYFDFAAGLTMTNAKFDAIFGGPPRKPESRLGQREMDLAKSVQV
                     VTEEIMMRMAKHVRKITGQKNLCLAGGVALNCVANGKLLKERVFENIWIQPAAGDAGS
                     ALGAALFGWYQYANNPRNADNINDTQFASYLGPDFTDAEIEAYLKSHDIPYKKHDRSA
                     MVQSVAGILADEKVVGWFQNRMEFGPRALGSRSIIGDARSTKMQSVMNLKIKYRESFR
                     PFAPSVLREKMTDYFDVDYDSKYMLLVADVKDERRIAMTEEQKKLFGIDKLNVPRSDV
                     PAITHVDYSARIQTVHQNDHGVYYELIKAFYDKTGCPVIVNTSFNVRGEPIVCTPEDA
                     FRCFMRTEMDYLVLGNFVLDKKEQKPVQDNKDWMNEFELD"
     gene            complement(13196..14722)
                     /locus_tag="PK754_04400"
     CDS             complement(13196..14722)
                     /locus_tag="PK754_04400"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HNB08776.1"
                     /translation="MKNIIRTVIISGIFGFYALRAQTYADHSASVFIPIDHWMYSYIT
                     LLQDRGHLASLNPSVRPYTRKEVFGAVKKLNQTDLSSAEKKWIQYIREELSDVAEDVA
                     CVDTTLIRTSWEGRLSLRRQQNDTHGDYFVKPNLEISSDNFAIHLRARIDHALRRDTN
                     YQGRKPSIGGAYLPDAYALLQWKSVRLTVGHYSTNWGPFENKSLIVSANPYPYERLEL
                     SWTTRRISIRSFVAQLDQAYNAGRYFSAHRMDIHLPHGINLGITESVIHGNADKTQPI
                     NWKYLNPINIFAEAQLNGGGDANENIAFDAYVPVGRFVFKGQFLVDDLILDGPDKPAP
                     NRKTSPDRLGFLITGIINNPGLPSSQAQLDYERVSSYTYNIKSSRPWQSYTYNGLGLG
                     SPRNDRDAWILTWRYFGFERWLLSLESGFFRQGERNLSSHDFTDSTMTVGKFPRGLVE
                     KTWRLGIQADYFHAHNLWTTCKISTDNVRNRSNKKTPLRHYLNVFVSLNYQIDRYLNI
                     "
     gene            complement(14719..15786)
                     /locus_tag="PK754_04405"
     CDS             complement(14719..15786)
                     /locus_tag="PK754_04405"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNB08777.1"
                     /translation="MPFIATFLAGLIIAVGVTPMLRKKLLARGVIDQPDARKVHTRAI
                     PRLGGVAIYLGFVVAVLTAYALKGAAFDEKITQFVGLLVAGTFIAVVGVFDDVYNVRA
                     IYKLFTQIAAAMILIAFDISMGSLSNPFGGSIELGVWNYPVTIFWVIGVINAINLVDG
                     LDGLASGVSLIVTMTIFLIALYLEKTYVALIALGLAGSILGFLRHNFYPAKIFMGDTG
                     SMFIGLMISALGLISSQKSAVSFAILVPFIALGYPVIDTALAIFRRARAGKSIFTADR
                     EHIHHILLSYGYSHPKTVIFLYVICLFFATMAFLFAVYNKSGTFTVGVLFFVLIFIFL
                     FIRMITDFKKKTEEDQETDDL"
     gene            complement(15808..16608)
                     /locus_tag="PK754_04410"
     CDS             complement(15808..16608)
                     /locus_tag="PK754_04410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNB08778.1"
                     /translation="MIDTLHTIRVLGFPTTTLDRRSLLSEINRWITVRDASRHLMALN
                     PIKVIRSRNEADLVRHIMQADMVYPDAFGISWAMSVFSGNKFKPIPGCDLMLDLFAMA
                     AQSGYKIFLLGASETVVSEAVQYIATHHAGAQIAGFRHGYFSSEDEKQKTVQQIIDTR
                     PDMVFVAMGALIQENWIQAIQKACTHQNISIPLLMGVGGSFDAITGHVPRPPRWMLRL
                     HLEWLFRLLQQPFRAPRMLALPKFATLVLLKKWFGINTDVQTDDFKKN"
     gene            complement(16605..>17369)
                     /locus_tag="PK754_04415"
     CDS             complement(16605..>17369)
                     /locus_tag="PK754_04415"
                     /inference="COORDINATES: protein motif:HMM:NF028171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNB08779.1"
                     /translation="STGVEEVYVFPNMGDGGLSTGAAFEVFRQQKGTCQPYRLHDVYL
                     GTSYNDSEIETALRQSGFPYKRYDNVEDEIGRLLSEKKIVARFNGGMEYGPRALGNRS
                     ILYHCQDRTVNAWLNDQLNRTEFMPFAPVLLKEDAHEYLTDYDPNRAYSAEHMTITYS
                     VTQKCADTAPAITHVDQTARPQLVTEDLNASYYRILKAYKQRTGHSILVNTSFNMHEE
                     PIVRSPEEAIKAYEQSRLHALAIGNYIVEVDWSKRP"
BASE COUNT         4785 a         3853 c         4082 g         4549 t
ORIGIN      
        1 agatgccaat atactctctg ccgcggcggt acataatcca tttccgcgcg taattttaaa
       61 ctatcgctct tatgcgccaa agaacgtgct ttgtgaattg tcagcgtcgg gataacatat
      121 tttccgtttt tacgaaatgc ttcggcaaat tgaataagtt gttctttagg ataggtatca
      181 ccgcttcgcg gattgagtaa acctaccagg ttgtcgaacg ataattgaga accttcagca
      241 tactcttcga atgggatcat atagggaacg tgaccttgca cgggaagacc ttgttggcgt
      301 gcttcgtcca aaacggccga ataaatatcg ggcgtaatcg tgacatacga aaatacagat
      361 tgataaccca tgcgtttcac gtcacgaacg gcgaatcgcg cttcatcggc attgaggagc
      421 atgccttgag tatggtaggc tacaacggat cctgtaacgg ccgggcccgt gctgtaaata
      481 cgcgggcctt gtagtttacc ggtatcagag agttttttga tcgcatgctg aaatgctgcg
      541 cccggcacat tacggaccgt tgtaattccg tttgcaagaa aaagcatcaa gtttttttca
      601 tgaatatgcg catagcagtc aatcaacccc ggtatgagat attgcccttt gccgtcgatg
      661 atcgaagctt ctttgggtat ggatacttca cgcgtattgc cgatagctgc gattttaccg
      721 tcggtgataa tgacagtttg atgatggagt attcgaaagc tatccatcgg tatgacatgg
      781 acgtttgtga acgcgtgtgt ttgagataga gctacaatcg agaatgtaga agacaaattt
      841 aaacctatac cgattaaaag aagtagtttc atacgaacct attaaaataa aattgaaaaa
      901 aaattattta gaaaacgtat acggcaaagc tctgtatcgt tcgttctgct ggagagcatt
      961 cggataggcg gttgaatttt taaaaagagt gcgtatggat tgtgcattga tatcgggaag
     1021 cccggttggc gcggtaacta catcaaaatg gagatgagga atacctagcg tataaccgct
     1081 attaccgctc aaagccacgg tgtcaccttt ggcaactgta gcgcctgttt tgaccaatgc
     1141 gccatgcttg cggatatgga tataacggga atacgtcgca tcgcgatgac gtatgacaag
     1201 tacattttcg tgacccaacg tgtgatcgct atcttcaaaa ttatcgctta taaatactac
     1261 ttcgccttct cgtgacgctg tcaatacagt tccgaccggc atatcaaaat cataggcata
     1321 tcggaaaact ccgttaaaat tattcggtga atggctccag atcgtgttgt tactttgaga
     1381 aaccttgtat cgtttaccta caggaaacgg cagcacgaaa tccaatgaat cggcaacaac
     1441 aactgtcgta tcggttgcct cgccatcttg gcaactacca aaaccaaagc ttcccaacga
     1501 aatacaagtg atcataatca aaacatgcat aacgactccg gttaagaaag gcttatccat
     1561 tgcggtgatt tccgtggtaa acgaattatt aatttgcgtg cttcaagatc aagtttgaca
     1621 gtgacgatat accacactac gctgccctca aaatccgacg atagtatctt tacagcatag
     1681 tcagccaatt cttcaaaagg cataggtcct ctttccttta atgtcgaaag tataaaaccg
     1741 cgtatcatat catatttgga tttgtctatg cgtacacctt gcttaccttg cggatgccgt
     1801 gttaatatgg tggatacttt tttcatgtat gatctccgcg tttttaggtg cctctagagg
     1861 ggcaaatgat aacgaataaa aacccatatc cgtctgattg tcgtttgtaa ggacacaaga
     1921 aattcatgta accaaagaat agatcttgat tgaaaaatac gacgatacgg cagcaagcaa
     1981 aagcaaatgg cgtcagaatg catcagatat agggtagagt gcatgaaatg gcctttgagc
     2041 cgccaaggtt actcaagcaa aaattaatat agagctatat tgttcattgg ttatcgtatg
     2101 ataaaaatca tcatgttttt atgaaaaaaa gttgcatatt cccctagttc aagcacatca
     2161 agaacataac ttcgttacca aaagggtgaa gattatgatc aaatttacca tcaatggccg
     2221 tacgcatgag gtttcagaag gaacccgtat tttagaggcc gcgtcctctg ttggaattca
     2281 gataccaaca ctatgtcatg atccgcggct caacccgagc ggcgtttgcc gtttatgcct
     2341 tgtagaaatt gaaggatctt ccaaaccggt accggcatgc cgtacggaga tcacgtccga
     2401 tatgcatatc gaaacgcata gcgctcatat cgaaaaatac agaacggatc tattgaaaat
     2461 gcttcatgcg cgttacccgc aagaagtggt ggaggctttc cctgagaagg aattgcatcg
     2521 tttgtttcaa cactacgtgc cgcgttttga agaaccggtt acggtcaaat cgtcatggaa
     2581 agataccaca catccttata tccgcattga tatgagtctg tgcgtagatt gccatcgctg
     2641 tgagcgtatt tgcagcgaag tgcagggaca attcgtatgg aatcccatcc agcgtggttt
     2701 tgcgaccacg atgacgccgg aaggtcgtcc gacgttattg gaaagcggtt gcgtaagctg
     2761 tggcgcgtgt gcggatacat gcccgagcgg tgcgctgatt gataagaacg tatttatgtt
     2821 gggggaaccg acttcatgga ctaaaacgac ctgtccgtat tgcggcgtgg gttgtgagat
     2881 gatggtcggg acccgcgatc aaaaaattgt caccgtaaaa ccgtctatgg acgggccggt
     2941 caataaaggc catctttgcg tcaaagggcg ttattcgcac ggttttgtcg gatcgggtga
     3001 tcgtatcaca gagcctatga tccgacgtga cggggcttgg caaaaagtat catgggatga
     3061 agcgtatgcc tttaccgcat ccgaactcaa gcgtatcagc gccaactacg gagcgagcag
     3121 tgtcggcgtt atcggttcgg cacgggctac taatgaagac aattatttga cacaaaaatt
     3181 tacccgcgtg gtattgcaga ccaataacgt agattcgtgt gcacgtgttt gccacgcacc
     3241 gagcgccaaa gccttgggta atatcctcgg gaccggcgcg gcgaccaatg cctttgatga
     3301 tatcgaacgc gctacgacca tttttatttg tggcgccaat gcgactgaaa atcatccgat
     3361 cgtgggcgcc cgcatcaaac aagccgcgtt gcgcggtgct caccttatcg tcatggatcc
     3421 gcgcgttatc gaattggccg aatatgccga cattcactta gcggttcgtc ccggtgccaa
     3481 tgtggctctg ctcaatgcga tggcttgcac cgtgatcgaa gagggcttga cggatgacga
     3541 atttatcctc gcccatgtga gcggttacga aaaattgcgc gaatttctca aagattttac
     3601 acccgaaaaa atagcggatc aatgcggcgt ggatgcggca acgattcgcc aggcggcgcg
     3661 tctttatgcg accgccaaac ccgcgatgtg tatgcacggt ctgggcatga ctgaacacac
     3721 gcagggtacg gactccgtga gcgggctgat caatcttgcg cttataaccg gtaatatggg
     3781 caaacccggg acaggcgtta atcctttacg tggacaaaat aacgtacaag gctcggcgca
     3841 catgggttgt gaaccgaccc atcttacggg atatggcacg gtggatgaag ggcgcacttt
     3901 atttgaagcg acatggaagg tgagtttacc gacggacaaa ggtcttaatc tcatgcacat
     3961 gttggatgcc gcagaaaaaa atcaattcaa agccatgtgg atcgtgggct atgacgtata
     4021 tatgactaat cccgatacgg atttttcacg tcgtgctttt gagaatatgg aactcgtcgt
     4081 gattcaggac atgtttatga atgaaacggc gcggttgttc ggtacggtgt ttttaccgtg
     4141 tacctcgtcg ttcgaaaagg acggaacctt catgaatgga gaacgccggg tgcagcgcgt
     4201 gcgtaaggct atcgaaccgt gcggccaaag caaaccggat tggttaatca tttgtgaatt
     4261 agctcatgcg atgggcgtac gtgaaggttt tgaatttgaa tccgccgaag atgtatggaa
     4321 tgaaatcaga cgtgtatgga aagcgggcta cgggattacc tatcctaaat tagaaaaagg
     4381 cggtatccaa tggccgtgtc ctaccgaaga tcatccgggc acggatattt tacatcgtac
     4441 gacgttcccg gttggcgccc gtgcggaagt gcaaatgcta cctttttctg cgccgcatta
     4501 cgatgcgcgg tacccgtgga cgcttattac cggacgcagt ttgttccatt tcaattcagg
     4561 tacgatgacc gggcgttcta aaaatgcgca gatcaaacgg ccggattatg tggagatagc
     4621 accgacggat gcggcccagt tgggcgtgca aaccggagat acactccgta ttaccagtga
     4681 aaacgggcaa gccttcgtac cggcgatcgt tacggatcat gtaaaacatc gcgaagtgta
     4741 tgcgacgttt caggaaccgg agaatttgct gaatgccgtt atcgggatgg aacgtgatga
     4801 caagacggcg acgcctaatt ataaggtgac gcaggtgcgt atcgaaaaag tatgatgagc
     4861 gagcgataaa ttttaccgaa aatcagaaaa aaaacttgct tttcggatcg gattatacta
     4921 tcatttcgcc gctttaacgg gcacgtagtt caattggtta gagcaccgcc ctgtcacggc
     4981 ggaagttgcg agttcgagcc tcgtcgtgcc cgcagtcaaa tccctgaaaa actcagggat
     5041 ttttcatttt aggggcctca tcaaaaagtg taaacccaaa aagtacggtc aaataataac
     5101 gccacgaacc cccgcttttt actttttggc gctcattgta atatgatccg tcttcactct
     5161 tgtatttggt tatcatcggg ccatatcctg taaatcggtc tatgccgtag gcggcattaa
     5221 aagtaagccg ggtagggtat acataaaatg aatacatctc tgtacgcaat tctattccca
     5281 catcacgttt ccatcggtcc atagtagatc gtgtgctcca ggcattgccc atgccaaatg
     5341 tcaaaccgcc gaaaatttta tcaaagtata aaggaccggc ttttttgtcg atgcggcgaa
     5401 tgagagggaa acgatatacg gcattgaaca agccgagata acgtccttcg atgctgtagt
     5461 aagaataacc tttcaatcct actaaacttc cgccgaaaaa attataaaaa ctatctactt
     5521 tggtcggaat aaaacctgta tgcaagcgtg cttccagagt atgatcgcca aaaggcaaac
     5581 gtataaattc agtccaggtg ttttcattcc gcaaataggc atcgcgcttg tatagtgttt
     5641 tattgctacc actgctcggt tcaatagcga atccattcaa gagattgtcc atgtaataac
     5701 cgacggatac actcatttga cggccgccgc gaggattgat ttctttatca atggccggcg
     5761 gaacattatt aaatttccac gtggttgaaa aatcattccc gtaataatat ttgattgctc
     5821 ctgaactgaa tgtaagaagg taattctcag catctaaatc atactctttg ttccgtatgc
     5881 gtacaaatga ttctatatgt gcataattaa tgcgtatatc gcgcggagca aataagctcc
     5941 aatccattcc ggcatctact tcgcgcagtt caaacgaatt ggtttgaatg gattcgtatg
     6001 gaatgtcatt accgggattg gtatcatcac gaaattcact tgaccgcgta atattataga
     6061 gttccaaaaa taatgtcgga ccgaagccgc tatactnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnngacc gcgtaatatt atagagttcc aaaaataatg tcggaccgaa
     6241 gccgctatac tcaaaaatac caaataaatc gcgctccata tccgggatat taaacatggc
     6301 cccgccgaaa aaagaggatt ttgacaatac atcgctggag taaaaataca cgcccggttt
     6361 gaaagtaccg tagtccaaac gtaaaacggg taggaatgaa aatttgagga atgtattttt
     6421 atatagcgcc gcggtatccg tccggggagg tatgacggta aatatgcgat gttccgaagg
     6481 ccacttgccg ggatatggtt tatcatttgg tttggacaat atattcgggt tttcaagtcc
     6541 gtaggcagtt tgttttgaat caaagccggt tgtgtccgca atcgaataca cacggaatcc
     6601 ggtgttctga tacaacgtgt aatacaagat gttgttctgg ccgacggccg gatagaatgc
     6661 gccaccggta acatttgtta tttgacgtat atcgccgcta tgcaaattca tagcgtacag
     6721 attaaatatt cctgtttcgt cagccgaaaa atacaaggtt tgcccgtctt gactgacgac
     6781 ggggctgcgt tcatctttgc gtgtttggcg caccggtttc caatggtttc cggaagtatc
     6841 cacgctgaaa atatcacggc catcgtcggt cgaagtatca aaaatcaacg tacgtccgtt
     6901 atcggtgtag cataggccgt aaatttgccg cccgtcatca aaatgagtta tacgccgcca
     6961 gtgcatcccg tcacgtacaa acatgttttt ggaaaaagta cttttttcat taagtacatc
     7021 ctcggcaaat cctacgatgc gaatgatatt attggttcca tcgccattga tgatagcagc
     7081 gagcgatgac ccgtccggtg aaaaagccgg agcaaatgca cgcagaccgc aggtgattcg
     7141 aaattctttg cgccgaacca tatcatacac gtagatgtca ttgtattgcg aatgatagtc
     7201 gttatcgtca gtattttttg cataggcgat gtaacggcta tcgggtgacc aacttaacgg
     7261 gccttccgct tttttgattt cgtgaatgat attcgagttt gacggatcca cgatatagag
     7321 agacgcctga ctgagatagt caaaaccgcg attggaaatc atcgcaagat attggccgtt
     7381 cggagaaagt gccggataaa aatttcctgt tccttctgtt gcgatttcac ggcctatacg
     7441 tgcatgtgcg cgtatatccg tcgtatgaag cgcgtattgc tctgtaatca ggttcttcca
     7501 ttggcggtag atcgtatcaa tatcatcgcc ggttgcaacc tttacggcgc gttcgaacga
     7561 ataaaaaagt ttggatgaat aatgagtgat ttttttaagc gatgaatcgc cgtaatgggc
     7621 cgcgatatag ctcactaatg cataaccgct attatatgcc gattcgttgc cgatactgtt
     7681 tttaccgaac gtgcccatct gattaagcgt cagcatttgt tcgcctaaga cgcgatcacg
     7741 caagatcata tcgcggtggg catcccaaaa ttcgtaatcc aaatcttttt gctggtactg
     7801 cgccacgcct tcggcaaacc acacgggcaa cataacgccg gcaacgggat aggaaacgat
     7861 gcggttcgga aaaccgcgca gtacgtcctg gcggcgctcc ttttcatatc ctatgtactg
     7921 caggtagatg ccgggaaaac ggcgtgatag cttcatcatc gcctgtatag aaatcatgtg
     7981 ggtaaattcg tgtgtgatta cattgcgcag ccaatgatgc gtaccgcgta attcaaagtt
     8041 taggttggac gcccagattt cgattttatt atcataatag taggcgccgc cgttggagta
     8101 gtcatccgta tcgcggatga tgatgtgtat cctttcgggg cgatggtcgt agagataggt
     8161 gatcgtttcg taaatagatt ccgcgatggc cgctgcttcg cgggcggtgc gttccgggcc
     8221 ttttcgggaa acgtcaagag ccgtgtatgg cgaatcatga taatgtacat caaaatgtgc
     8281 cgaacggatc gtaaaccatt gcaattcgcc atgcgaccaa gcttccggtt gtgcataacc
     8341 ggatcgaacc aaaatcaaaa cgaaaaaaag aatttcgaag actaaaaatg tctttttcat
     8401 ttttttataa accgaataat aagttcaccg ttattgtaat ccgcaccttc gggctccaac
     8461 gcggccatgg aatcgggaat agtatgggtg cgcaaaaagg atcctatttc gatcatgatt
     8521 tcattgcctc tgcgatgaag ggaaaccggc gtattttgta catccaaaaa gggcgctttg
     8581 agtttcatta acttttcctt tttggtattc acaaattgta cggggcgtga attgtaaaac
     8641 accgatgcgg gatcggcttg gttgtacagc gcattcccta gagtacgtaa atcgtttagt
     8701 ccgtgaatct cgtgatgaaa aaaaggcgca tgggcgatcg gtatcggcgt gaaatcacgg
     8761 gttatcgcat gaatatactt tttttgcgtc tcatgccaaa tgcgaaaata gcctttttgc
     8821 gtttcgtccg gtagaatacg attgataatc acctgatcca cgaccatgcc aaataaattg
     8881 aggtaggtca acgcgcgctg cgtttcattc atgatcatag attcgggttg catcaccaga
     8941 cgagtcgtag tgcgtgattc tttttcaaaa agatcacgca ttgccaaaat tttttcaagt
     9001 atggcttccc aggaacgcgc cacgtcttcc ggtgcgacga cgacgcgcaa cgatttagac
     9061 atacgcgatg ctgggcgtat cacgggagaa acgatatatt tttcgatcac gcgtgttatt
     9121 tttaaagcca gtgatacgat ttgcggcaaa ctcaagagcc gtaaactttc gccggtcggc
     9181 gccatatcta cgatgatgac tttataatcg gacttatcgt gaaaatcccg gattcgcaga
     9241 agactgaaaa gattatccat tcccggaaga atggccagtt caccggagac ggctccgtca
     9301 gcgccaagcg tctgaataag attggaaaaa tagtcgcgga tatgccccca attgctgtca
     9361 agttcggcat ggggatcaat atgaaccgca tcgagattgg gtgcaaccgg agtgggtgta
     9421 gcatgaatcg gcaggcctaa taaatcaccg atactgtgag cgggatccgt actcagaagt
     9481 atcgttttag taccgcgatc cgcacatcgc aacgccgtag cagcagcgat cgtagttttg
     9541 cctacgcccc ctttacccga aaagaaaatg atacgtttac gtgtcaaacg attcccttac
     9601 ggcttaacta tatctaataa atcttcttcg acggtttttt gtggaaattc aaccatgcga
     9661 ccgatttccg cgccgtttcg caaaacgatc atggtcggta cgcgtttgat gttgtattgg
     9721 gccgttacac ctagagaatc tttttttgtc cgatccacgg ctaataagcg gtaagtcaag
     9781 tttggatttt tggcggcgtc cagtgttttg agaaatgagg gtacatgatg tttggaatcg
     9841 ccgcaccacg ttcccaatac aatcagtata tcggtaggtg tactcatgcg gtgaagcgtt
     9901 tctattgcac ttcctgccgg attgtagacg tcgtagtttt tttggaaaac cggcatttcc
     9961 tggaacaatt cttcgcgcgt aatattgccg atgagcatca tttcatcttt taccgttttg
    10021 gcatgggaaa cggttttagg aacggaactg caaccgatga ttccaaatgg tgataaaatc
    10081 agaatgatcg tatacagaga cttaattatg cttttcatcg gatttctttt ttcgtgtggc
    10141 aaacttggcg acccaaatgc tcatttcata aagtaccagc atcggcgcgg caacgacaaa
    10201 catggtgatc atatcggtcg tcggagtgat gaccgcggat acaagaaggc acgcaacagc
    10261 agcgtggcgc cagtactcgc gcataaattg gggtgacaga atgccgattt tggcaagaaa
    10321 aaaagcaatc acgggcattt caaacgtcgc tccgctgata agcataaatc cggatacaaa
    10381 actcaagtac tgatcaacat caatcatgtt ttcaaccaca ataccgagtt cggtatcaat
    10441 atttcccata ctcagaagga aattaagcgt gatcggtatg acgacaaggt aggcaaacgt
    10501 cgccccgagc aggaaaaaaa acgtgataaa aaaaataagc ggaatggcaa aacgtttttc
    10561 tgattttttg agtgccggag agatgaatcg ccataattga tacgcaatga caggaatgct
    10621 caatacgata cctgcgataa ggcctacttg tattttgacc atcatcagac cttgcacctg
    10681 cgtgatcacg ggccgcatca actggatcgg cggtttaaga tcggggatga tcatcggtgg
    10741 ataaagcaac cacttgatga tcgtattact ataaacaaac gatatcgcca tcatcagcaa
    10801 aacggcaagc acgattttta tcaagcgatc ccgcaattca ccgagatgat ccagaaaagt
    10861 catatcgccg ccggtagaac cgggtagcgc gcgtttttct tcgtcagcca taaaaatcta
    10921 aaacattctt tcgtagtgtt cgggtgtttt taccggggat tcttttttgg tccaataggt
    10981 gtttgtttta ggatcaattt gtttttttag caaatctctt ccaaacaaac gcgtgatcag
    11041 gcctaccgga acaaagaaca atgtgaacat gataagtagt atgacccgtg aaacaaaata
    11101 tccgataaaa attgaaaaaa tcgtagcgcc ggtatacaga ggatatatca ggttaggtgc
    11161 gatcgcggat atcaatccga ctaaaactcc gggtgccgcc caatacatcc aaacgtcacg
    11221 ctctttatac caaagccatc ctgcgatgag taataaaatc gttgttaaaa taaatccgaa
    11281 acgccgagcc gaagcagggc ttcgatccat gtgctgaatt tcaaatcgta tatcgcgaat
    11341 aatttctttc atagatcgtc cttaattaat cgagttcaaa ttcattcatc cagtctttgt
    11401 tgtcctggac gggcttttgt tcttttttat ctaaaacgaa atttcccaga acgagataat
    11461 ccatctccgt acgcataaaa caccggaacg cgtcttctgg cgtacataca atgggttccc
    11521 cgcgcacatt aaaggacgta ttaacgatca ccgggcaccc tgttttatca taaaaggctt
    11581 tgatcagttc gtaatacacg ccatgatcat tttgatgaac ggtctgaatg cgtgcagaat
    11641 aatctacgtg ggtgatcgcc ggaacatccg aacgaggcac attaagtttg tctataccaa
    11701 aaagcttttt ctgttcttcg gtcatggcga tgcgccgttc atctttgaca tcggctacga
    11761 gcagcatgta cttgctgtcg taatctacgt caaaataatc cgtcattttt tcacgcaaaa
    11821 cgctcggcgc aaacgggcgg aatgattcgc ggtatttgat tttcaggttc attacggatt
    11881 gcattttcgt agaacgggcg tcgccgatga tactgcgtga acccaatgca cgcgggccga
    11941 attccatacg gttttgaaac caacccacga ccttttcgtc ggctaggatg ccggcgacgg
    12001 attgtaccat agcagagcga tcgtgttttt tgtaagggat atcgtgggat tttaaatagg
    12061 cttcaatttc ggcgtccgta aaatcagggc cgaggtacga agcaaactgt gtatcattta
    12121 tattatcggc gttgcgcgga ttgttggcat attgatacca gccgaaaagt gcggcaccca
    12181 atgcgctgcc tgcatcgcca gcggccggtt gtatccatat attttcaaaa acgcgttctt
    12241 tgagcagttt accgttggcc acgcaattga gcgccacccc gccggccaaa cagagatttt
    12301 tttgaccggt gattttccgt acatgtttgg ccatacgcat cattatttct tcggtgacca
    12361 cttgcacgga cttggctaga tccatttcac gctgtcccag gcggctttcc ggtttacgcg
    12421 gcggtccccc gaagatcgcg tcaaatttag cattggtcat tgtgaggcct gcggcgaaat
    12481 caaaataatc catattcatc cgaaatgacc cgtcttcttt gaggtcaatc aggtgtttgt
    12541 agatcagatc gacgtattta ggttcgccgt acggagcaag gcccatgact ttgtattcgc
    12601 ctgaattaac tttaaatccc gtataatacg taaatgccga atacagaaga ccgagcgaat
    12661 gggggaagtg aatctcatag tctatttgta atttattgcc gcgcccggtt ccaaaactgg
    12721 tcgtagtcca ttcaccgaca ccatcgattg tcaaaaaggc agcgtcttga aacggagacg
    12781 ggaaaaaggc ggctgcagcg tgcgattcgt gatgttccgg ataaatgatt tttcctttaa
    12841 agcccggtaa ctcctccatc agaagctcct gcatccagag tttttttctg atccactgcg
    12901 gcatggcctt gataaacgaa ttgaatccta tcggcgcata agcaaaataa gtacgaagca
    12961 atcgttcaaa cttaacgagc ggtttttcat aaaacgcgac cacgtcaatt tgcgacatat
    13021 caataccggc tgtttttaga caaaattcca cggcgtgttt tggaaaatta tcgtcgtgtt
    13081 tttttcgtgt aaaacgttct tcttgtgcag cggcgatgat ctttccgtca cgcaccaaag
    13141 ccgcagcgga atcatgataa aaacaagata tgccgagaat gttcattgct tagtcctata
    13201 tgttaagata tcgatctatc tgataattaa gggaaacaaa tacattcaag taatgccgca
    13261 gaggagtttt tttgttagag cggttgcgga cattgtcagt tgatatttta caagtagtcc
    13321 aaagattatg tgcgtgaaaa taatcggcct gaatacctaa gcgccatgtt ttttcgacta
    13381 aacctctggg aaatttgccc acagtcatgg tcgaatcggt aaaatcatga ctgctcaaat
    13441 tgcgctcgcc ctggcgaaaa aaaccgcttt ccaaagacaa tagccaacgc tcaaaaccaa
    13501 aatagcgcca cgtcagtatc catgcatcgc gatcattgcg cgggctgcca aggcctaatc
    13561 cattataggt gtaggactgc catgggcgcg acgatttaat gttgtaggtg taagagctga
    13621 cacgttcgta atcaagctgg gcttgagatg atgggagacc gggattatta ataatacctg
    13681 tgatcagaaa tcccagccga tccggtgatg tttttcgatt cggcgccggt ttgtccgggc
    13741 cgtcgagtat caaatcgtcc acgaggaact gacctttaaa cacaaaccgg cctaccggta
    13801 catacgcatc aaaagcgata ttttcattcg catcgccgcc gccgtttaat tgcgcttcgg
    13861 caaaaatatt gatcggattg aggtatttcc aatttatcgg ttgcgtctta tcggcatttc
    13921 catgaataac agattcggtg atacccagat taataccgtg aggcaggtga atatccatcc
    13981 ggtgcgcaga aaaataccgc cccgcattgt acgcttgatc taactgcgca acaaaacttc
    14041 gtatagatat ccgtcgtgta gtccaagata gttcgagccg ctcataggga tacggattgg
    14101 cagaaacaat gaggctttta ttttcaaaag gtccccagtt tgtcgaataa tgaccgacgg
    14161 ttaatcgaac actcttccat tgaagaagcg cataggcatc gggaagatat gcgccaccga
    14221 tacttggttt acgcccctga taattggtgt cccggcgcag tgcatgatct atgcgcgcgc
    14281 gcaaatgtat ggcgaaatta tccgatgaga tttcgagatt gggtttcaca aaataatcgc
    14341 catgcgtatc gttttgctgg cgacgcagcg ataaccggcc ttcccatgaa gtgcgaatca
    14401 gagtcgtatc cacacaggcg acatcttctg caacatccga taattcttcc cgaatatatt
    14461 gaatccattt tttttcagca gaagaaaggt cagtttgatt caattttttt acagccccga
    14521 atacttcttt tcgcgtataa ggccttactg aggggttgag cgatgccaaa tgcccgcgat
    14581 cctggagaag agtgatgtaa gaatacatcc agtgatctat gggaataaaa acggacgcgg
    14641 aatgatcggc ataagtttgt gcgcgaagtg catagaaacc gaagataccg gagatgatta
    14701 cggtgcggat gatatttttc acagatcgtc tgtttcctga tcttcttcag ttttcttttt
    14761 aaaatccgta atcatgcgga taaaaagaaa aataaatatg agtacaaaaa acaaaacgcc
    14821 gaccgtgaat gtgccggatt tattatatac ggcaaagagg aatgccatcg tcgcaaagaa
    14881 aaggcatatg acgtataaaa aaataaccgt tttgggatga gaatatccgt acgataaaag
    14941 aatatgatgt atatgctcac gatccgccgt aaaaatactt ttccctgcgc gtgctctccg
    15001 aaaaatagcc agtgccgtat caatgacggg atagcccaaa gcaataaaag gaactaatat
    15061 cgcaaagcta acggccgatt tttgagagct gatcaaaccc aacgcagaaa tcatcaaacc
    15121 gataaacatg cttccggtat cgcccataaa aattttagcc ggataaaaat tatgtctcaa
    15181 aaaaccgagt atactcccgg ctaatcccag tgcgatcaac gccacgtatg ttttttcaag
    15241 atacagagcg atgaggaaaa tggtcatggt aacgataagt gaaacaccgg atgccaatcc
    15301 atccagaccg tccacgaggt tgatcgcgtt aataacaccg atgacccaaa aaatggtaac
    15361 cggataattc catacgccaa gctcaataga tccgccgaat gggttggata aactgcccat
    15421 agaaatatca aaggctatca aaatcatagc cgccgcgatt tgcgtaaaaa gtttgtaaat
    15481 cgcacgaaca ttatacacat catcaaaaac accaacgacg gcgataaatg ttcctgcaac
    15541 gagcaggcct acgaattgag tgattttttc atcaaaagca gcgcctttaa gagcataggc
    15601 cgttaataca gcgactacaa atcctaaata gattgcaacc ccgccaaggc gcggaatcgc
    15661 tctcgtgtga actttgcgtg catccggttg gtcaataacg ccgcgtgcca aaagtttttt
    15721 gcgaagcatc ggtgtaacac cgacggcgat gataagtccg gcgagaaatg tagctataaa
    15781 aggcaaagtg aattcctgaa atgaatttta attttttttg aaatcgtcgg tctgcacatc
    15841 cgtattgata ccaaaccatt ttttcaaaag cacaagggtg gcaaatttgg gtagcgccaa
    15901 catacgcgga gcacgaaacg gctgttgtaa taatcggaaa agccactcca aatgcaggcg
    15961 taacatccat cgcggaggac ggggaacgtg ccctgtgatc gcgtcaaaac tgccgcctac
    16021 acccatgagc aaaggtatgg agatattttg atgagtacaa gctttctgta tcgcctgtat
    16081 ccagttttcc tgtatcagtg cacccatcgc tacaaatacc atgtcagggc gggtatcaat
    16141 gatttgctga actgtttttt gtttttcatc ttcacttgaa aaataaccgt ggcgaaagcc
    16201 ggcaatttgc gcaccggcgt ggtgcgtcgc aatgtattgt acggcttcgg atacaacggt
    16261 ttctgaagcg cccagaagga aaattttgta tccggattga gccgccatgg caaaaaggtc
    16321 caacatcaga tcgcaaccgg gaatcggttt aaatttattt ccggagaaaa cactcatagc
    16381 ccaactgata ccgaacgcgt cagggtatac catatccgcc tgcataatgt gacgaacgag
    16441 gtcggcttcg ttgcgtgaac ggatcacttt gatgggatta agcgccatga gatggcgtga
    16501 agcatcacgc acggtgatcc agcggttaat ctccgacagc agagatcgcc gatccaatgt
    16561 ggtcgttgga aaaccaagta cgcgtatggt atgcaaggta tcgatcatgg gcgctttgac
    16621 cagtccactt cgacgatata gttaccgata gccaaagcgt gcaaacggct ttgttcgtag
    16681 gctttgatcg cttcttccgg ggagcgcacg atcggctcct catgcatatt gaaactggta
    16741 ttgaccaaaa tcgaatgccc tgtacgctgc ttgtaagctt ttaggatgcg ataatacgag
    16801 gcgttaagat cttcggtgac caattgcggc ctggccgttt gatcaacgtg cgtaatggcc
    16861 ggcgccgtgt cagcacattt ttgggttaca ctgtacgtaa tggtcatatg ttccgcagag
    16921 taggcgcggt tgggatcata atcggtgagg tattcatgcg cgtcttcttt aagtaacacc
    16981 ggtgcaaacg gcatgaattc cgtacgattc agctgatcgt tgagccacgc attgaccgta
    17041 cgatcctggc aatgatagag aatcgagcga ttgcccagcg cgcgcggacc gtactccatg
    17101 ccgccgttaa aacgcgcgac aatttttttc tccgagagca atcggcctat ttcatcttct
    17161 acgttgtcat agcgtttgta ggggaatccg gattgccgga gcgccgtttc tatttcactg
    17221 tcgttgtatg aagtgcccaa atatacatcg tgcaaacgat agggttggca cgttcctttt
    17281 tgctgacgaa atacctcaaa agccgcgccc gtagacaatc cgccgtctcc catgttgggg
    17341 aaaacataaa cttcttcgac gccggtgga
//
LOCUS       DAOQNQ010000040        17343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1160_length_17343_cov_5.431976, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000040 DAOQNQ010000000
VERSION     DAOQNQ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17343
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1160_length_17343_cov_5.431976"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2345
                     /locus_tag="PK754_04420"
     CDS             <1..2345
                     /locus_tag="PK754_04420"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNB08780.1"
                     /translation="IIIIDQGKTFDPRNAKAPDMAEYIKIGKKGGLGIFMLKKMMDEI
                     DYQVTSEGNELRLVKYRTQGKRGSRAKWLRDVNLRNRYAIISSAALTLLVLIGYVLYE
                     KIQTKNMENEILKRITAISATMASTSADLMASYDDLSMFQIAQRLKKDNEQMIVDIFV
                     VDRNNYIFAASNRNSLGKYPMPENYETLSFDGSDSPQLPDSVAQQFENPELLTLVKTY
                     KYTQEDLPTFDIATPVYRSNIISQDNLLGYAHVLVREQTILNEMTNLRILGIVLALLV
                     LLVGYTGIAILLARIISPFQKLSDWIRSAGHESVSDDVDIDTTNEVGEIAQAFSDMAS
                     KFRKAQIGLVQQKQLQKEIQVAQEIQHMLLPVSFPEVQGYDLSTYYESAKEVGGDLFD
                     FIKVDDDTIGIVVADVSGKGVPGSLVMTMIRTALRLEARGNKNPADVLARVNEFVVDD
                     MKKGMFVTMFYIVLDSRNREIHYASAGHNPMILHRVSTKQTYYLNPRGFPVGISLPDR
                     KLFGQSIQTDSIKLQEDDILIAYTDGITEAMNHERDLYSEERFLETIRRHAHLDAKAF
                     VENMRNDLNEFTQGAPQSDDITLVAIKENLAAGDVLFNLRKQLIDMVAEGMSVKEACA
                     KTRISTSTYYKYKKKYDELGEAGLRNKSEGSNIEAKHFSIEQRAKIFDVIRKHPEFGP
                     KMISNTLNSEEYSFTTIPEKRIYDELVRLRLNTEEKRQAFVSQKAKTKRFKMPGTPLL
                     TMEGEVIKNDGSNANSIVDRIMQQRQQAIESEKKTEPAEA"
     gene            2511..3884
                     /locus_tag="PK754_04425"
     CDS             2511..3884
                     /locus_tag="PK754_04425"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNB08781.1"
                     /translation="MKSLWNTRIRAFSAAALVLAALSEVYAGGFSIYEQGGRATSQGG
                     AVVARPWDASAAFYNPAGLAFYGGAPGTYRFYVGVTPVQSLSKFTGLDSFPGRGVHEE
                     AKAKWFPPFYAYGVYQINENMAAGLSITTPFGLGTEWKNPNNTYSGRFRSLMANIEAV
                     YISPVISYKVNNMIGVGAGVDFVYSRVELRRHQGIDIFDGEATKKYDVADIYLKGEDK
                     LSMGFHVSAYAKINENLSFGADYKHSIKNDYEGHARFRQIKTGYVAIDNSVAANFAND
                     LFGGMRQEGNTSVTFPSSLVIGAAYKPMEKLTVEADFALAGWSVFKEVEILFPNQRSI
                     DATTGDTIKVKSILKENYDDTWQIRLGAEYDVNERLQVRAGYIFDKTPAPTETVNPLL
                     PDANRNDFSIGVGYKINDNWHVDAAYMAVIFSERSTKGKNVDNFDGVYNSHVNLFSLG
                     FGYTFGN"
     gene            3909..5309
                     /locus_tag="PK754_04430"
     CDS             3909..5309
                     /locus_tag="PK754_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08782.1"
                     /translation="MNSKFKNTMASNILLLFVVFAFAACSVDYPEVSEQKLPRITTSG
                     TADFTKFATVGNSLTAGYQSSGLASRFQVNSYPAIIARQLGMDVTDDSTSEFQQPLAS
                     DSGTQGILKLNFSPTGPLPSTTYAITEPFINLGLSRAYDNLAVPGAFVYDYLNASTPA
                     TGWGGLILGSANTLYTPILRGLGTQHQQLRSLHPTFVIWWEGHNDILGYATRGAGVNT
                     TPGSSQGAPPWTPRNSTEAGGMGIPFNFTTMYTASLDSVVATGADVIVGNIPYVTDIP
                     YFTTVYPSSSVFKLNNDSIVTITNALTGSKAKLYFEEDVTGPADSIVYMLLPVSSLIG
                     KTNVSGFSGKPYGLTPTARIQGRYTLTVSEVNHIKSIIDGYNSIISAAAATHGIQVVD
                     FNSYFTTIKSNGILLPNGSKIGTTFISGGLFSLDGVHPTTFGYAVVADKWIESINSKF
                     NSNVPRVNLMDYYQFK"
     gene            5450..6706
                     /locus_tag="PK754_04435"
     CDS             5450..6706
                     /locus_tag="PK754_04435"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNB08783.1"
                     /translation="MAQGQIRYNINPTPSLGGKVFVFFNGTANYAKSTPGNAANDREN
                     LEQRVIDIINNASTSLDMAAYELNSLNIVVALCKAHERGVRVRIIMDDEAAPNNNEEL
                     WKYVRTLLSYRYQVPWMTDAGWPIVKSKKNLFKARTAQMHNKFVVADYLSADTTDDMV
                     LTGSYNFTITGMVSMQNVVRIQNAKAAQIYTEEFENMWGSDTEQPDTTKAAFHQYKGA
                     LKNDPIVFGGKIGVYLAPMNKHKTRPNYLEMIADIITREVQHDIKICSFSFSTKIDVD
                     EAIKEKAEERGVNVKAVFDRSLGKGNWSLYKSMIGDTSARVVWKKKAEAYLAYEDRQL
                     HHKYLIIDAESPDTSDVPVVITGSFNFSKNANEANDDNFLVIRDRAIANQYLQEFYAR
                     FEAAKKLELSQKETNQTEKLITDDDE"
     gene            complement(6715..8304)
                     /locus_tag="PK754_04440"
     CDS             complement(6715..8304)
                     /locus_tag="PK754_04440"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HNB08784.1"
                     /translation="MARVVSQLKKMIFAERNSKKHIAAIDIGTNSFHLIVVELLGNGK
                     YRIVDREKENVRLGSGSSDMKMIAPDAIERGVTALKRFSEIAKVYKAPIRAVATSAVR
                     EALNQAQFVKKIKDATGINIEVVSGNEEARLIYLGVLQALPVFNHKIIAMDIGGGSVE
                     YIVGARGEAQYLNSLKIGCIRLTQRFFDKGVIKPKKIQECREYIRGFVSAVAREVRDR
                     KIEFAVGSSGTILNLANMIRTNRSGISSDAQLNNFSFTKKELDRVVQNILKAKTVAQR
                     IKIIGLDIKRADIIVAGALVLQESFDLLKIKQMVVSEYALREGIIHDYINSRTNTGGH
                     HPLSNIRYRSVMHLAENFHYDAEHAHQVTRLALSIFDQTVKIHKLGSTEREFLEFAAI
                     MHEVGFFVSHAQHHRHSYYLIRNAELAGFTDQEKEIIANIARYHRKSHPKSKHEGFVN
                     LSLENQRLVCKLAGVLRIADGLDRTHSQSITSLRCKIQPKHILIKAHCKSAGTLEQWA
                     VNMKKGLFEESLGRSVTVRIN"
     gene            complement(8304..8801)
                     /gene="sixA"
                     /locus_tag="PK754_04445"
     CDS             complement(8304..8801)
                     /gene="sixA"
                     /locus_tag="PK754_04445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HNB08785.1"
                     /translation="MDVFLMRHGFAEDVGGKIKTDFDRPLTGEGVKLMQAEARGLKRL
                     DIRFNVIMCSPLVRAKQTAEAVAEVLGHKKVVVCDGLAIPASTAELMQAFKPFQMDYS
                     VLLVGHMPDVGRLAGFFTGVPRLAIPFKKGAICKIEVERIHPSPSGEIRWFMTPRQLK
                     FIGES"
     gene            complement(8805..9725)
                     /locus_tag="PK754_04450"
     CDS             complement(8805..9725)
                     /locus_tag="PK754_04450"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNB08786.1"
                     /translation="MEASWYIPDLLPTMGASEAMQRIMRHRWISWKSLCDAVKENQSA
                     EQIHDFRVASRRLRQAMACLVFYSSSAAKLKHHNHAIKTLADHAGEVRDSDIMLHKVQ
                     ILKSKHSKIQSANWDYIENQIETKRLRSLKQFQNTIQKTDLNIDISWVSSESETKNDP
                     IGVTAVDLAVQWIKERSEALWITESDCGDPLSSGTLHAFRIEAKRLRYSLEMWSGILD
                     GNISRTLKILKKLQDYLGLIHDCDTIIKTLRKSAQIYKTENEIHNAMIIKTIHYVVRE
                     RISITHRFYSYWNSDVRRVLQKQINHFVME"
     gene            complement(9746..10963)
                     /locus_tag="PK754_04455"
     CDS             complement(9746..10963)
                     /locus_tag="PK754_04455"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNB08787.1"
                     /translation="MTHADLVTKLVQKTDSKIVLLVMDGLGGIRTKQNPKTELERANI
                     PNLHTLAKESVCGRIMPVSYGITPGSGPAHFSLFGYNPADDNIQIGRGVLEALGLDMK
                     LQPGDVATRCNFATMDTKGLLTDRRAGRIPSEECERLCAMLGAKIKKIEDVKVIIKPG
                     EQYRFVVVFRGKRLHGNILDTDPQVTGAKPLKAVPQDKASTRMSKIADKFIAAANKIL
                     KDETKANTLLMRGFSGMPHVPSMSERFGLAPACIASFPLYRGVASVVGMEILPTGMDI
                     ADEFQTLKENWDKHDFFFIHIKKTDSYGEDGNSEGKMHIMEEVDKHIPMLRELGPAVI
                     MVTGDHSTPPPMKSHSWHPVPFLLHSDVCSFDDANAFTETECSKGQLGNFPSYYIMEL
                     ALANAGKLKKFGA"
     gene            11192..11413
                     /gene="rpmE"
                     /locus_tag="PK754_04460"
     CDS             11192..11413
                     /gene="rpmE"
                     /locus_tag="PK754_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNB08788.1"
                     /translation="MKPGIHPTYPVSNVTCSCGNSFKTRSTVGDIRVEICSNCHPFFT
                     GQTKLIDTAGRVEKFNKKFAKYQDAQKKA"
     gene            11554..12531
                     /locus_tag="PK754_04465"
     CDS             11554..12531
                     /locus_tag="PK754_04465"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HNB08789.1"
                     /translation="MSNNPITPKTITVGGQAVIEGVMMRSADAVATAVRKPNGQIVIR
                     REPFTSLLHRYRFLNIPVLRGSIAIIEMLIIGTSALQYSADVSMSNDEEGSSPKKSSR
                     LELFLTTAFALGIGVLIFFAGPISLTNYFIGADQNAWSFNLVAGTIRAAMFITYVWAI
                     SQMKDIFRLFQYHGAEHVSIYAFENKDALIPENGLKYDTPHPRCGTSFLMFVMIASIL
                     SFAIIDSLYIAYIGPLGLWNRLALHLPCVPIVAGLSYELIKLSGKYYHLSWARIFMMP
                     GLWLQRITTKKPDITHIEVAMAALQSALGEEAVRAYNAGQVTGAMSVHE"
     gene            12543..13613
                     /gene="prfA"
                     /locus_tag="PK754_04470"
     CDS             12543..13613
                     /gene="prfA"
                     /locus_tag="PK754_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNB08790.1"
                     /translation="MIDQLEKASIRFNEINGLLSRPETVSNNNLFRSLSKERSALEPI
                     VEKFETYKKVNSDILGNKDILHNSDDADLRDMARAEMNDLESKKQILESELKYLLIPK
                     DPHDDGDAILEIRAGTGGDEAGIFAGDLYAMYQYYVEKKGWALTLVDFTEANQGGFKE
                     IIFEVNGTGAYGKLKFESGVHRVQRVPKTETQGRVHTSAATVAVLPVLEEDDSEVVIN
                     PNDLRVDIYRSGGKGGQNVNKVETAVRLTHLPTGIVVQCQDERSQLKNREKAMKVLKS
                     RLYDLRKREHDAKIASQRKSMVGSGDRSEKIRTYNFPQNRLTDHRIGLTIYDLEGVMG
                     GKIDDIVEKLQLAENEELLKAG"
     gene            13634..14725
                     /locus_tag="PK754_04475"
     CDS             13634..14725
                     /locus_tag="PK754_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HNB08791.1"
                     /translation="MNKRYHFIDLFRGWAVLVMIETHVFNAWMDKSLRSTEWFSYLNF
                     INGFVAPSFLFVAGVSFVMVAWNNWYSFTSFNPGYRKQLRRFFVIMLIGFALHLPRLD
                     WSQGWPHIVTEDLVSFFQIDVLQTIAISLILLQLLMLWTRGITVFFIVLFIITWSALL
                     ITPFLWQKDFSSSVHPLIANLLNGKNNPLFPLFPWCVFLWSGGLVGGLFMRYSTSDKE
                     HKIVPAIALIGVSLFAIGYISDISPIRFFEYESFWLTSPNWVLMRLGILLILFSFFWL
                     LDYKNWHTSPQVLLFGRQSLFAYISHLVLIYSITGEKASISIFDQNLPPWLTLILWIV
                     VCAAVYALTHLWVQLKPGLTARFKPSVKK"
     gene            14827..16032
                     /gene="mqnE"
                     /locus_tag="PK754_04480"
     CDS             14827..16032
                     /gene="mqnE"
                     /locus_tag="PK754_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HNB08792.1"
                     /translation="MKHINTVYDHSLIKSMLHTSGLDAIYQKVMDHERLSFEDGVQLF
                     ETRDLTSVGYMANIVRERMHGDRSFFVRNRQLNPTNVCEYSCMFCSFYRREGEEGGYT
                     MTMEQIAQRVKDGLKQGIQEIHITSGLHPGLPFSYYTDMLSTIKSVAPHIHIKGFTAV
                     EIEYYANHYNMSMRAVLEALMKAGLEAMPGGGAEIFAQRVREKICDEKATAEGWFKVH
                     GMAHELGLMTNATMLYGHIERYDERIDHLIRLRELQDDSLAKKTKGSFSTFIPLKYQH
                     ENNRLKKLPQVSAFEDLKVVAVSRLMLDNFPNIKAYWVVIGKNMAQTALSYGANEIEG
                     TILEETIMSMAGTDSASGMTTSELIKLISDAGRQPIERDALYNVIKEFSTEDIAHAHR
                     NPVMLPVLN"
     gene            16086..16778
                     /gene="mqnB"
                     /locus_tag="PK754_04485"
     CDS             16086..16778
                     /gene="mqnB"
                     /locus_tag="PK754_04485"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HNB08793.1"
                     /translation="MRILIVSATEAEIKPLLVHYKAVHPDKLGLWRSLTSHSHTIDFL
                     VTGIGMINTAYSLGKQLQISSYDWAINLGIAGAYSEVHALASIVRVDTDILAEMGATT
                     ADGQFENLQRMGFPLHSYDNKAIYNEIENPNDLQNWSRLAQTPKVRGITVNTVTGNTE
                     RILWNRNNFTPDIESMEGGAFALACLRAGQPYVQYRSLSNFVEPRDRSRWKINEAVAA
                     LSTFTIQLLEKI"
     gene            16781..>17343
                     /locus_tag="PK754_04490"
     CDS             16781..>17343
                     /locus_tag="PK754_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HNB08794.1"
                     /translation="MKFTLGYSTCPNDTFIFDAMVHKKIDTEGIEFEPFLADVEELNQ
                     KAFRCELDITKLSYHAFLYLLKDYALLTSGSALGHRCGPLFISARPINVHGIAGDKIL
                     IPGKLTTANFLLQFFLGKTVQAQPVLFSDIEKNLLNGSAEAGVIIHEGRFTYQAKGLH
                     LIQDLGDYWETKTGMPIPLGAITIRKT"
BASE COUNT         4903 a         3816 c         3612 g         5012 t
ORIGIN      
        1 ccattatcat tatcgatcag ggcaaaacat tcgaccctcg aaacgccaaa gcgcctgata
       61 tggctgagta tatcaaaatc ggcaaaaagg gcggtcttgg tattttcatg ctcaaaaaga
      121 tgatggatga gattgattac caggtcactt ccgaaggtaa cgaacttcgc cttgtcaaat
      181 atcgtacgca aggtaaacgc ggcagccgcg ccaagtggct ccgtgatgtg aatttacgta
      241 accgctatgc tattatatca tccgccgctc tcacgcttct cgtattgatc ggatatgtgt
      301 tatacgaaaa aatacagacg aagaacatgg agaacgaaat cctcaaacgt atcacggcga
      361 tttcagcaac catggcttcg acaagcgcgg atctcatggc cagttacgac gacctttcca
      421 tgtttcagat tgcgcagcgt ttgaaaaaag acaacgaaca gatgatcgtg gacatttttg
      481 tagttgaccg caacaactat atttttgctg cttccaatcg caatagcctc ggcaaatatc
      541 caatgcctga aaattatgaa acactatctt tcgacggttc ggattctcct caacttcccg
      601 atagtgttgc gcaacaattt gaaaaccctg agctacttac attagtaaaa acctataaat
      661 atactcagga agacttgccc acgttcgaca ttgcaacacc ggtttaccgt tccaatatca
      721 tttcgcaaga caatttgctc ggctatgccc acgtattggt gcgcgaacaa actattctca
      781 atgaaatgac caatttgcgc attttaggga ttgtcctggc attacttgta ctccttgtcg
      841 gctataccgg tatcgctatt cttctcgctc gcattatcag tcccttccaa aaactaagtg
      901 actggattcg cagcgccggc catgaatccg tttcagatga cgttgacatt gatacgacca
      961 atgaagtcgg tgaaatcgcg caggcttttt ccgacatggc atccaaattt cgtaaagctc
     1021 agataggtct cgtacagcaa aagcagttgc aaaaagaaat acaggtcgct caagaaatcc
     1081 agcacatgtt gttaccggta tcatttcctg aagttcaagg ctacgatctt tctacttact
     1141 atgaatcggc taaagaggtc ggcggtgatc tttttgactt tataaaagta gatgatgata
     1201 cgatcggtat cgtagttgcc gacgtatccg gtaaaggtgt tccgggttcg ctcgttatga
     1261 cgatgattcg taccgcattg cgcctcgaag cccgcgggaa caaaaatcct gctgatgtac
     1321 tggctcgcgt caatgagttt gtcgtggatg atatgaaaaa aggcatgttc gtcacgatgt
     1381 tttatatcgt tcttgattca cgtaaccgtg aaattcacta tgccagtgcc ggtcataatc
     1441 ccatgattct tcacagggtt tcgaccaaac aaacatacta cctcaatccg cgcggttttc
     1501 cggttggcat ctcgcttccc gatcgaaagc tctttggtca atcaattcag actgacagta
     1561 ttaagctaca agaggatgat attctcattg cttatacgga cggtattacg gaagcaatga
     1621 accacgagcg ggacttgtac agtgaagaac gtttccttga gaccatccgc cgacacgcgc
     1681 atttggatgc caaagcattt gtcgaaaata tgcggaacga tcttaatgag tttacgcaag
     1741 gtgcgccgca aagcgacgat attacgcttg ttgcaatcaa agaaaattta gctgccggcg
     1801 atgtgctttt caatttacgt aaacaattga ttgatatggt agctgaaggt atgagtgtga
     1861 aagaagcctg cgcaaagaca cgtatatcta cttcaactta ttataagtat aagaaaaaat
     1921 atgatgaact cggtgaagca ggactccgca ataaatcgga gggttcgaat atcgaggcta
     1981 aacattttag tatcgaacaa cgcgctaaaa tttttgatgt catacgtaaa caccctgaat
     2041 ttggccctaa aatgatcagc aacacactca attcagagga atatagtttt acaacaatac
     2101 ccgaaaaacg catctacgat gaattagttc gcctgcgtct caacacggaa gaaaaacgtc
     2161 aggcttttgt cagtcaaaaa gcaaaaacga agcgtttcaa aatgcccggc acaccacttc
     2221 ttaccatgga gggtgaagtt atcaagaacg acggatctaa cgcaaactcc atcgttgatc
     2281 ggatcatgca acagcggcaa caagccattg agtctgagaa gaaaacggaa ccggctgaag
     2341 cgtaaacact gtatgtttta gaaaacatag aattgtattg actaattttc actgtttcgt
     2401 tagtattgtt ttataatact tgtcttgtaa tccattcgtt ttttcagttc tttagatttt
     2461 tgttcaatta ctttcacttt ccaataacaa ggaaattcag gaggatccca atgaaatccc
     2521 tttggaacac acgtatccgg gcgttcagtg cggctgctct ggtgttagcc gccttatcgg
     2581 aggtttacgc cggcggtttt tcgatttatg agcaaggtgg tcgggccaca tcccaaggtg
     2641 gcgctgtagt cgctcgccca tgggacgctt cggcggcgtt ttacaatcca gccggtttag
     2701 cattttatgg aggtgccccc ggaacatatc gtttttatgt cggcgttacg cccgtacaat
     2761 cattatcaaa atttaccggt ttggacagct ttcccggacg cggtgtacat gaagaagcga
     2821 aggcaaaatg gtttcctccg ttttatgcct atggcgttta ccagatcaat gaaaacatgg
     2881 ccgccggttt atctatcaca actccgttcg gtttaggaac agagtggaaa aaccccaata
     2941 atacatatag tggccgattt cgttcactta tggctaatat agaagctgta tatatcagcc
     3001 cagttatttc atataaagtc aacaatatga tcggcgtagg agccggagtt gattttgttt
     3061 atagccgcgt cgaacttcgt cgccatcagg gcattgatat ttttgacggt gaggcaacta
     3121 aaaagtacga tgttgccgat atttatctga agggcgaaga taaattatct atgggttttc
     3181 acgtttctgc atatgctaaa ataaatgaaa atttatcatt cggtgcagac tataaacact
     3241 cgatcaaaaa tgattatgaa ggccatgccc gattcaggca gatcaaaaca ggctatgtag
     3301 ctattgataa tagtgtagct gccaactttg ccaatgattt atttggaggg atgcgacagg
     3361 aaggcaatac gtcggtgaca tttccaagct cacttgtcat cggagctgcc tataagccga
     3421 tggaaaaact tactgtggaa gcagattttg cattagcagg ttggagcgta tttaaggaag
     3481 ttgaaattct cttccccaat cagcgttcaa ttgatgcaac cacaggtgat acgataaaag
     3541 taaaatccat cctaaaagaa aattatgatg atacatggca aatccgtctt ggtgccgaat
     3601 atgacgtaaa tgaacggtta caagttcgtg ccgggtatat tttcgataaa acacccgccc
     3661 cgactgaaac ggttaatccc cttttgccag atgcaaaccg caatgatttt tcaatcggtg
     3721 taggctataa aattaatgat aactggcatg tagatgcggc ctatatggcc gttattttta
     3781 gcgaacgcag taccaaaggt aaaaatgttg acaactttga cggagtttat aactctcatg
     3841 tcaatctgtt ttcgctcgga ttcggatata catttggaaa ctaataaaaa agtcatattg
     3901 gagtcactat gaattcaaaa tttaaaaaca caatggcaag caatatttta ttgctgtttg
     3961 tggtttttgc ttttgctgcc tgctccgttg attatccgga agtcagcgaa cagaaacttc
     4021 cgagaataac taccagcggc acagccgatt tcacaaaatt cgcaaccgta ggtaacagtt
     4081 tgactgcagg ttatcaaagt agtggactag ctagtcgttt tcaagtaaac agctaccctg
     4141 caatcattgc tcgccaactc ggtatggatg ttacagacga ttcgacctct gaatttcagc
     4201 aacctctagc ttcggattcg ggtacgcaag gcatcctgaa acttaacttt tcgcctactg
     4261 gaccacttcc aagcacaacg tatgccatta ctgaaccttt cattaattta ggcttaagta
     4321 gggcgtacga caatcttgct gttcccggag cttttgtata tgattactta aatgcaagca
     4381 caccggctac tggttggggt ggcttgatct tgggaagcgc caatacatta tatactccaa
     4441 ttttacgcgg attaggaaca caacatcagc aacttcgttc gttgcaccct acatttgtta
     4501 tttggtggga gggccacaat gatattttag gttacgctac ccgtggcgcc ggcgtaaata
     4561 ctacaccggg ctcatctcaa ggcgctccgc cctggactcc aagaaattcg actgaagctg
     4621 gcggaatggg cattcctttc aattttacga cgatgtacac tgcttcattg gatagtgttg
     4681 ttgcgaccgg tgcggatgtt atagtaggaa atatacccta tgtgacagat atcccttatt
     4741 ttactacagt ttacccttca tcgagtgttt tcaaactaaa caatgactcg atcgttacca
     4801 ttacgaacgc tctaaccggc tctaaagcta aattgtattt tgaagaagat gtaacggggc
     4861 ctgctgatag tattgtttat atgttgttac cggtaagtag tttaataggt aagaccaatg
     4921 tttcgggatt tagcggaaag ccttacggtt taactcccac cgcaagaatt cagggacgtt
     4981 ataccctgac cgtatcagaa gtcaatcaca taaaatcaat tattgatggc tataattcga
     5041 taatctcggc agccgcagca acccacggta ttcaagtcgt tgacttcaac tcatatttta
     5101 ctaccattaa aagcaatgga attttgctac ccaacggtag caaaattgga acgactttca
     5161 tatcgggcgg tcttttcagt ttggatggtg ttcaccctac gactttcggt tatgccgtcg
     5221 ttgcggataa atggattgaa tccatcaatt ccaaattcaa cagtaatgtt ccacgcgtaa
     5281 acctgatgga ctattaccaa ttcaagtaat actggtatcc ttaataaaaa accccgtttc
     5341 tgatgaaacg gggtttttta tttgtgtatc atgtgctgat tgaatatttt gtttcatgtt
     5401 taatagaaac gctaaatttg tagtttcaat ccttgtggtc catttttctt tggcacaggg
     5461 ccaaattcgc tataatatca accctacccc ttctttgggc ggcaaggttt ttgttttttt
     5521 taatggtacg gccaattatg caaaaagcac tccgggaaat gcagccaacg accgagagaa
     5581 tcttgaacaa cgggtcattg atattatcaa taatgcatca acaagcttag atatggcggc
     5641 ctatgagttg aacagcctta atatcgtcgt ggctctatgt aaagctcacg agcggggtgt
     5701 gcgcgtacgt attatcatgg atgacgaagc cgcgccgaac aataatgaag aactttggaa
     5761 atatgtacga actttgctga gttatcggta tcaggtaccc tggatgactg atgctggctg
     5821 gcccatcgtt aaatccaaaa aaaatctctt caaagcacgt acggcacaga tgcacaataa
     5881 atttgttgtg gccgattatt tgagcgccga tacgactgat gatatggtat taaccggctc
     5941 ctataatttt acaattacag gcatggtttc catgcaaaat gttgtccgaa ttcaaaacgc
     6001 aaaagctgct caaatttata ctgaagaatt cgaaaacatg tggggctccg atacagaaca
     6061 gccggatacg acgaaagccg cctttcatca atacaaaggt gctttaaaaa acgatcccat
     6121 tgtttttggc ggaaaaatcg gcgtatatct ggcgcctatg aataagcata aaacgcgccc
     6181 taattatctg gaaatgattg cagatataat tactcgtgag gtgcaacatg acatcaaaat
     6241 atgttcgttt tcgtttagta ctaaaatcga tgttgatgag gcaataaagg aaaaagccga
     6301 ggaacgcggc gtcaatgtaa aagctgtatt tgataggtcg ctggggaaag gaaattggag
     6361 tttatataag tcaatgatcg gtgatacgtc ggcgcgcgtc gtttggaaaa aaaaggcgga
     6421 ggcctatttg gcatatgaag accgtcaatt gcaccataaa tatttgatca tagacgctga
     6481 gtcccctgat acgtcagatg tccccgtcgt aattacgggt tcttttaatt ttagcaaaaa
     6541 tgccaatgaa gccaatgatg ataacttttt ggtcatacgt gatcgtgcga tagccaatca
     6601 atatttacag gaattttatg cacgttttga agcggccaaa aaacttgagc tttcacaaaa
     6661 agagactaat caaactgaga aactgatcac ggacgacgac gaataaagat gctgctaatt
     6721 gatacgcaca gtaacagaac gccctaacga ctcttcaaac aatccttttt tcatattaac
     6781 tgcccattgt tccagcgttc ccgccgattt acagtgcgct ttgatcaaaa tatgtttagg
     6841 ctgaatttta caccgaagcg acgtaatgct ttgactatgc gtcctatcca atccgtcagc
     6901 aatacgcaac acacctgcta atttacatac taagcgctga ttctcgagtg aaagattgac
     6961 aaaaccttca tgctttgatt tgggatggct cttgcgatgg tacctcgcta tattggctat
     7021 aatttctttt tcctgatcgg taaatcccgc caactccgca tttcgaataa gatagtacga
     7081 atggcgatga tgctgtgcgt gcgagacaaa aaaacccacc tcgtgcatga tcgccgcaaa
     7141 ttcaaggaat tcccgttccg tgctgcccag tttatgtatt ttaacagtct gatcaaaaat
     7201 actaagcgcc aatcgggtta cctgatgtgc atgttctgca tcgtagtgaa aattttcagc
     7261 cagatgcatg acactacgat aacgaatgtt tgataagggg tgatgcccac ccgtattggt
     7321 gcggctgttt atgtaatcat ggataatacc ttctctcagc gcgtattcgg aaactaccat
     7381 ttgcttgatc ttaagtaaat caaatgattc ctgtaaaacc aaagcaccgg ccacaataat
     7441 gtctgctctt ttaatatcta acccgatgat ttttattcgc tgcgctaccg tttttgcttt
     7501 taagatattc tgaactacac gatccagttc ttttttggta aaagaaaaat tattcaactg
     7561 ggcatccgat gaaatcccgc ttctattggt tctgatcata ttggctaaat tgagaattgt
     7621 acccgaactg ccaactgcaa actcgatttt gcgatcacgg acttcccgtg ctacggcgct
     7681 tacaaatccg cgaatatatt cgcggcattc ctgtattttt ttaggcttaa taacaccttt
     7741 atcgaaaaac cgctgtgtta aacgaataca tccgatctta aggctgttca gatattgcgc
     7801 ttctcctcga gctccaacga tatattcgac actgccgcca ccgatatcca ttgcgataat
     7861 tttatggtta aaaaccggca atgcctgcag cacccctaga taaatcaacc gtgcttcttc
     7921 attgccggaa acaacttcaa tatttatgcc ggttgcatct tttatttttt ttacaaattg
     7981 cgcctgattg agcgcttcgc gcacggcgct ggttgctacg gcgcgtattg gcgctttgta
     8041 tactttagca atttcagaaa agcgctttaa cgccgtaacg cccctttcta tagcatccgg
     8101 agcaatcatt ttcatgtcag agctaccgct gcccaaacgt acattttctt tttccctgtc
     8161 aacgatacga tacttcccgt tacccaataa ctcaacaact atgagatgaa atgaattagt
     8221 accaatatcg atcgcagcaa tatgcttttt ggaattccgc tcagcgaata tcattttttt
     8281 gagttgcgaa acaacacgtg ccatcaggac tctccgataa acttgagctg acgcggcgtc
     8341 ataaaccagc gtatttcacc gctcggcgat gggtggatgc gttccacctc gattttgcag
     8401 attgcacctt tcttaaaagg aatagctaac ctcggtactc ccgtaaaaaa accggctaat
     8461 cttccgacat ccggcatgtg gcccacaagt aaaacgctgt aatccatttg aaacggtttg
     8521 aatgcctgca taagttcggc tgttgacgca ggaatggcca aaccatcaca tacgaccacc
     8581 tttttgtgtc ccaatacttc ggcaacagcc tctgccgttt gtttggctcg taccaatggg
     8641 ctgcacataa tgacattaaa acgtatatcc agtcttttga ggccgcgtgc ttcagcttgc
     8701 atcaatttga caccttcgcc ggtcaacggc cgatcaaaat cggttttaat cttcccccct
     8761 acatcttcag cgaaaccgtg tcgcattaaa aatacatcca taacttactc catgacaaaa
     8821 tggttaattt gtttttgcag aacacggcgc acatcactat tccaatacga ataaaaccga
     8881 tgggtaatcg aaatacgttc ccgtactaca tagtgtatcg ttttgattat catagcgttg
     8941 tgtatttcat tttctgtctt ataaatttgc gctgactttc taagtgtctt tattatcgta
     9001 tcgcaatcgt gaattagtcc gagataatct tgtagttttt ttaaaatctt taatgtgcga
     9061 gatatgttac cgtccagaat cccgctccac atttcgagcg aataacgtaa ccttttggct
     9121 tcaattcgaa aagcatggag cgttccggat gataatggat cgccacaatc tgattcggta
     9181 atccacaacg cttcacttct ttcttttatc cactgaaccg ctaagtccac tgcggttact
     9241 cctatgggat cgtttttagt ttcgctttct gaagaaaccc atgatatgtc tatatttaaa
     9301 tccgttttct gtattgtatt ttgaaattgc tttaatgatc tcaaacgttt ggtttcaatt
     9361 tgattctcta tataatccca attcgcagat tgaatttttg agtgcttgga tttaagaatc
     9421 tgaaccttat gcaacataat atcactatcg cgcacttccc cggcgtgatc agctaatgtt
     9481 ttgatcgcat gattgtgatg tttcagtttc gcagccgaag aagagtaaaa tacaaggcag
     9541 gccatcgctt gacgtaatcg tcgcgaggct actctaaagt cgtgtatctg ctctgcactc
     9601 tgattttctt tgacagcatc gcataaggat ttccatgaaa tccagcgatg acgcatgatt
     9661 cgctgcatcg cttcagatgc gcccattgtc ggaagcaaat ccgggatata ccacgaagct
     9721 tccatagtaa ttttatttct ataggttacg cgccaaactt cttcaatttt cctgcattgg
     9781 ccaatgctaa ttccatgata taatatgagg gaaaattacc caattgacct tttgagcatt
     9841 cggtttctgt aaaggcattt gcatcatcaa aactacatac gtccgaatga agtaaaaatg
     9901 gaaccggatg ccaactatga cttttcatcg gcggcggtgt cgaatgatct cccgttacca
     9961 taattacggc aggaccgagt tcgcgcaaca tcggaatgtg cttatctact tcttccatga
    10021 tatgcatctt tccttcagaa tttccgtctt cgccgtagct atcggttttt ttgatatgaa
    10081 taaagaaaaa atcgtgttta tcccaatttt ctttcaatgt ttgaaattca tcagctatat
    10141 ccatacccgt tggtaatatt tccataccca cgacactggc gacaccacga taaagaggaa
    10201 aactggcaat acacgccggc gccaaaccaa aacgttcgga catcgaaggt acatgcggca
    10261 tacccgaaaa tccgcgcatg agcagcgtat tggccttggt ttcatccttt aggattttat
    10321 ttgctgcggc tataaattta tcagctattt ttgacatacg tgttgacgct ttatcttggg
    10381 gaacggcttt cagcggtttg gctccggtta cttgtgggtc ggtatcaagt atattgccgt
    10441 gcagacgttt cccgcgaaat acaacaacaa agcggtattg ttcgcccggt ttaatgatca
    10501 ctttgacgtc ttcaattttt ttaatcttag cacccagcat cgcacaaaga cgttcgcatt
    10561 cttccgaggg aatacgacca gcgcgccgat cagtaagcaa tcctttcgta tccatcgttg
    10621 cgaaattaca acgtgtggca acatcacccg gttgcaactt catatctaag cccaaagcct
    10681 caagcacgcc gcgaccgatc tggatattat catccgcggg attatagcca aataatgaaa
    10741 aatgtgcggg gccgcttccc ggggttatgc cgtaagaaac cggcataata cgtccgcaca
    10801 cggattcttt ggccaacgtg tgcaaattcg gaatattggc acgctctaac tctgttttag
    10861 gattttgttt tgtacgaatc ccccccagcc catccatgac taacagaacg attttagaat
    10921 ctgttttttg aacaagcttc gttaccaaat cggcatgtgt cataacctac cctcatcaaa
    10981 atgaacgata ttatttttga cctttaaata taacaacatt acctgattat tcaaatgttc
    11041 attgtcagga accgggcact aaaagatgtg taaaaaccat aaccacaaga aaacgggaat
    11101 aatataaaaa aaatattgtt tttaatacga tcattggtta ctattgcgcg cttaagaagc
    11161 atatatataa caatttaaca taaggacagc catgaaacct ggtattcacc ccacgtatcc
    11221 cgtgtcaaac gttacctgct cttgcggtaa tagttttaag acccgttcaa ccgtcggcga
    11281 tattcgcgtc gaaatctgct ccaactgtca tccttttttt acagggcaaa ccaagctgat
    11341 tgacaccgcc ggtcgcgttg aaaaattcaa taaaaaattt gccaagtatc aggacgctca
    11401 gaaaaaagct tagtttagat tacgtctgac aaagggctgt ctcaaaagaa ttttacctaa
    11461 gacgaaacgt cgaatttgtt ttttgtaaaa acacagattg cgtttcttat atggaataaa
    11521 atcttttggg aaagcccttt ttatttacca taaatgagta acaatcccat aacacctaaa
    11581 acgattaccg ttggcggtca agccgtcatc gaaggcgtaa tgatgcgttc agcggatgct
    11641 gtcgctacag ccgtaaggaa acctaacggg caaatcgtca tacgacgcga accctttaca
    11701 tctttgcttc atcgctatcg ttttctcaat attcccgtct tgcgcggcag tattgctatt
    11761 attgaaatgc ttattattgg cacttcggct ttacagtatt cagcagacgt atccatgagc
    11821 aatgacgaag aaggctcttc tcccaaaaaa tcttcccgac tggaattgtt tttgaccacg
    11881 gcttttgctt taggcatcgg ggttttgata ttttttgcag gccctatctc cttgaccaat
    11941 tattttattg gtgccgatca aaatgcatgg tcttttaatc ttgtagcagg aacaatacgc
    12001 gccgccatgt ttataaccta tgtttgggcg atcagtcaga tgaaagatat ttttcggctt
    12061 ttccagtacc atggcgccga gcatgtctct atctacgcgt ttgaaaataa ggacgcattg
    12121 attcctgaaa atggattaaa atacgataca ccccaccccc gttgcgggac cagttttttg
    12181 atgtttgtta tgatcgcatc catcttgtcc tttgctatca ttgatagttt atatatcgcc
    12241 tatatcggtc ccttggggct gtggaatcgt cttgctttac atttgccctg tgtaccgatt
    12301 gttgcgggtt tatcatacga attgatcaaa ctttccggta aatattatca tctgagttgg
    12361 gcacggattt ttatgatgcc cggattatgg ctccagcgca tcacaacaaa aaaacctgat
    12421 atcacgcaca tcgaagtggc tatggcggct ctgcaatcgg cgctgggcga agaagccgta
    12481 cgcgcgtata acgcaggcca ggtcaccggc gccatgagcg tacacgaata acatagaaaa
    12541 aaatgatcga tcaactcgaa aaagcatcca ttcgttttaa cgaaatcaac ggacttttat
    12601 cacgaccgga aacggtaagt aacaataatc tgtttcgatc gctgagcaaa gaacgcagcg
    12661 cgctcgaacc tattgtggaa aagtttgaaa cctataaaaa agtgaacagt gatattctcg
    12721 gtaacaagga tatacttcac aactccgatg atgctgattt gcgcgatatg gctcgtgcag
    12781 agatgaacga tctggaatca aaaaaacaaa tactcgaatc ggagctaaag tatcttctga
    12841 ttcccaaaga cccgcatgac gacggagacg ctatcctcga aatccgtgcg ggaacaggcg
    12901 gtgacgaagc cggtattttt gcaggcgatc tctatgccat gtatcagtat tacgttgaaa
    12961 aaaaaggttg ggcgctcaca ttggttgatt ttaccgaagc caatcaaggc ggatttaaag
    13021 aaatcatttt tgaagtaaac ggtaccggcg cttacggtaa acttaaattt gaaagcggcg
    13081 ttcatcgcgt gcaacgtgta cccaaaactg aaacgcaggg ccgcgtacat acttctgcag
    13141 caactgtggc cgttctccct gttctcgagg aggatgactc agaagtcgtc attaacccca
    13201 atgatttacg cgttgatatc tatcgctccg gcggcaaggg aggccagaat gttaataagg
    13261 ttgaaaccgc cgtgcgcctt acacacttac ctaccggcat cgtcgtccaa tgtcaggacg
    13321 aacgctcaca attgaaaaac cgcgagaaag cgatgaaagt gctcaaatcg cgtctatatg
    13381 atctccgtaa acgagagcac gacgctaaga tcgcttctca gcgcaaatcc atggtcggct
    13441 ccggcgatcg cagcgaaaaa atcagaacgt ataattttcc tcagaatcgc ttaacggatc
    13501 atcgtatcgg acttacgata tatgacttgg aaggcgttat gggaggcaaa atagatgata
    13561 tcgtagagaa attacaatta gctgaaaacg aagagctact taaagccgga taatccttgc
    13621 aggtgcgatg cgtatgaata aacgttatca cttcattgat ctttttcgcg gatgggctgt
    13681 ccttgtaatg atagagaccc atgtttttaa tgcgtggatg gataaaagcc tgcgttctac
    13741 tgaatggttt tcctacctga attttattaa cggttttgtt gcaccatcat ttctttttgt
    13801 tgccggtgtt tcctttgtca tggttgcttg gaataattgg tattccttta cttcatttaa
    13861 tcccggttat cggaagcaac tgcgtcgttt tttcgtaatt atgttaatcg gttttgctct
    13921 tcatcttccg cgattggact ggagtcaggg atggccgcat attgttacgg aggaccttgt
    13981 cagctttttt cagattgatg tattgcaaac tatcgccatc agcttgattc ttttacaact
    14041 attgatgctg tggactcgcg gcataaccgt attttttatt gtgcttttta ttattacatg
    14101 gtcggccctt cttattacgc cgttcctttg gcaaaaggat tttagttcat ccgtacatcc
    14161 cctaatagcc aatttactaa atggaaaaaa caatccttta tttccacttt ttccgtggtg
    14221 tgttttcctt tggtccggcg gcttggtcgg cggccttttt atgcgctata gcacttctga
    14281 taaggaacat aaaatagtac cggcgattgc actgatcggg gtatctttgt ttgccatcgg
    14341 ttatatttcg gatatttcgc ccattcgttt ttttgaatat gaaagttttt ggttgaccag
    14401 tccgaattgg gtactgatgc gtctgggtat attacttatt cttttttcgt tcttctggtt
    14461 acttgattac aaaaactggc acacatcacc acaggtatta cttttcggac gacaatctct
    14521 ttttgcatat atttcacacc ttgtacttat ctactcgata acgggcgaaa aagcgtccat
    14581 cagcatattt gatcagaatt taccgccctg gttaaccctt attttgtgga ttgtcgtttg
    14641 tgccgccgtc tatgcgctga cgcatttgtg ggtacaactc aaacccggtc ttacggcacg
    14701 atttaaaccg tccgtcaaaa agtagtttat acttgtttat attggcggct tattttagtt
    14761 taccaatgtt tcaaaaaaaa ttgaaactta aattatgata aacactatat ccaaaggcag
    14821 atattaatga aacatataaa taccgtatat gatcactctc tgataaaatc catgctgcac
    14881 acctccggtt tggacgccat ttatcaaaaa gtgatggatc atgaacggct ctcttttgaa
    14941 gatggcgttc aattgtttga gacgcgtgat ctgaccagcg taggttacat ggccaatatt
    15001 gtccgggaaa gaatgcacgg cgaccgatca ttttttgtac gcaaccgcca gcttaacccc
    15061 accaacgtat gtgaatattc gtgcatgttt tgttcgtttt accgccgtga aggcgaagaa
    15121 ggcggctata ccatgacaat ggaacaaatc gcacaacgtg ttaaagacgg attaaagcaa
    15181 ggcatacaag agatccatat cacttccggc ttacatccgg gactcccctt ttcatattat
    15241 accgatatgc ttagtacgat caaatcggtt gcaccgcata ttcacattaa aggttttaca
    15301 gccgtcgaaa tcgaatacta cgccaatcac tataacatgt ccatgcgtgc tgtgctggag
    15361 gctttgatga aagccggcct cgaagccatg cccggcggtg gcgctgaaat tttcgctcag
    15421 cgtgtccgtg aaaaaatatg tgatgaaaaa gccacggcgg aaggttggtt taaagtccac
    15481 ggtatggcgc atgaactggg cctgatgact aatgcgacga tgctttatgg tcatatcgaa
    15541 cgttatgatg aacgtattga ccacttaatt cgtttacgtg aactccaaga tgattcattg
    15601 gcaaaaaaga caaaaggatc atttagtaca tttatcccgc tcaaatacca gcatgaaaac
    15661 aaccgattaa agaaattacc gcaggtcagc gctttcgaag atttgaaagt cgtagcggta
    15721 tcgcgcctga tgctggataa ttttccaaat atcaaagctt attgggtagt tattggtaaa
    15781 aatatggcac agacggcgct cagctatggc gctaatgaaa tcgaaggaac aatcctcgaa
    15841 gaaacgatca tgagtatggc cggaacggac agtgcatctg gaatgaccac gtcggaattg
    15901 atcaaactca tatccgatgc gggccgccaa cccattgagc gcgatgcgtt atacaatgtg
    15961 ataaaagaat tttcgactga agatatagcg cacgctcacc gtaatccggt catgttacct
    16021 gtgctcaatt gatttccaaa aactttggcc accgttcggc taccataatt catctttagt
    16081 atcccatgag aatactcatt gtttccgcga cggaagctga aatcaaaccc ctattggttc
    16141 attataaggc cgtacatccc gataaacttg gtttgtggcg ctcacttact tcacactccc
    16201 atacgatcga ttttctggtt accggcattg gtatgatcaa tacggcatat agtcttggta
    16261 aacaattaca aatatcatct tatgattggg ccataaatct cggaatagcc ggtgcctata
    16321 gtgaagttca tgctttggcc tctattgtac gtgtagatac ggatatactc gcagaaatgg
    16381 gcgctacgac ggcggatgga caatttgaaa atctacagcg aatgggattt cccttacatt
    16441 cttacgacaa caaggcgatc tacaacgaaa tcgaaaatcc caacgatctg caaaattgga
    16501 gccgattggc gcaaacaccc aaagtaagag gaatcacggt caataccgtt accggtaata
    16561 cagaacgcat tctgtggaat cgcaataatt ttactccgga tatcgaaagc atggaaggcg
    16621 gcgccttcgc actggcttgt ttacgggcgg ggcaacctta tgtacaatat cggtcgcttt
    16681 ccaattttgt cgagcctcgc gaccgaagtc gctggaaaat caacgaagct gttgcggcgt
    16741 tgagcacctt tactatccaa cttttggaga agatctgaaa atgaagttta cgctgggtta
    16801 ttctacctgc cccaatgata cgttcatttt tgatgccatg gttcataaaa aaatcgacac
    16861 cgaaggaatc gaattcgaac cttttcttgc agatgtcgaa gaactcaatc agaaagcgtt
    16921 ccgctgcgaa ttagacatta caaaattaag ttatcatgcc tttttatatt tgttaaaaga
    16981 ttacgcctta ttgacatccg gaagcgcatt ggggcaccgc tgcggtcctt tatttatttc
    17041 ggcacgccct attaatgtcc atggtattgc cggtgataaa atattgatcc ccggaaaatt
    17101 gactacagcc aattttttac tgcagttttt tttgggaaaa accgttcagg cacaacctgt
    17161 tcttttttca gatatagaaa aaaacctatt aaacggttcg gccgaagcgg gagtgattat
    17221 tcacgaaggg cgttttacat atcaggccaa aggccttcat ttgatccaag atttgggtga
    17281 ctattgggaa acaaaaacag gcatgcctat tccgctgggc gccataacca tcagaaaaac
    17341 gtt
//
LOCUS       DAOQNQ010000041        16439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1254_length_16439_cov_5.726135, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000041 DAOQNQ010000000
VERSION     DAOQNQ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16439
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1254_length_16439_cov_5.726135"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PK754_04495"
     CDS             <1..231
                     /locus_tag="PK754_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08795.1"
                     /translation="NDGVNNGIDINPQNVQAKTVISVMRQIGNPSNIVRNYEKVLSMS
                     EFMSESDYIQLLKEADKQLVAYRDLLTDKLNP"
     gene            387..1253
                     /locus_tag="PK754_04500"
     CDS             387..1253
                     /locus_tag="PK754_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08796.1"
                     /translation="MAAAGSGRYQFVFGEHQLTFQGRGRYEDYNTSFERHAWTSHAST
                     EYVYASGAWVWSLNTNWRTQQYGLSGPDVRATLYNLQSRGEYYFGQRMGAFAELRFER
                     NISKVDHENRFASRAGVIGLVWSSAEYLKVFTGFLTERFFASTTDPLTYPSKNNGWRN
                     GMETGLDYSGTWIINGRYIASWRVSKYTLNYSLEQEAHIVIGKHINERWGLYFLADYV
                     RRDIPRENKITRNLLYTNSVQDNRAHIKLSYETAANQRLFIRAAYSQTEFTQKVEPFR
                     YKTIVLGYEVNL"
     gene            complement(1285..1626)
                     /gene="ytxJ"
                     /locus_tag="PK754_04505"
     CDS             complement(1285..1626)
                     /gene="ytxJ"
                     /locus_tag="PK754_04505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HNB08797.1"
                     /translation="MTEIQSPEMLDALLEKSLNTTVLLFKHSNSCGTSAYALEQVETF
                     LSEYPEWNSRSGMIVVQTHRPISNRVADLFAITHQSPQIFVIQNKEVLWHKSHHSITS
                     DAIKKIVLSIV"
     gene            1720..1791
                     /locus_tag="PK754_04510"
     tRNA            1720..1791
                     /locus_tag="PK754_04510"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1753..1755,aa:Glu,seq:ctc)
     gene            complement(1833..3155)
                     /locus_tag="PK754_04515"
     CDS             complement(1833..3155)
                     /locus_tag="PK754_04515"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNB08798.1"
                     /translation="MAFKQLHVIEPILQALKSEGYTKPTPIQEQAIPLILNHKDLLGC
                     AQTGTGKTAAFAIPILQLLFQSSSVHIPRKTHALILTPTRELAIQIGESFTAYGRHTG
                     LRHAVIFGGVSQHAQTQALKVGVDILIATPGRLLDLMQQGFVHLQQLKIFVLDEADRM
                     LDMGFIHDVKKIVGKLPSKRQTLLFSATMPAEIQKLAHSILSHPEQVSVTPVSSTAEK
                     IEQAVYFVNKPDKKNLLIHVLRNPEITSALIFTRTKHGADKVTRDLLKAGVTAEAIHG
                     NKSQNARQRALGNFKNAKTRVLVATDIAARGIDVDNLSHVINYEIPNIPETYVHRIGR
                     TGRAGMSGTAISFCDPEERAYLKDIQKLIGKTIPVMDMPIAVTPAKAAANTLHDHGIK
                     PQQTKQHGRPQHKTGSSDRRTKQKTQHQTGKPKDHRSWFKNTFKKRED"
     gene            complement(3452..4396)
                     /locus_tag="PK754_04520"
     CDS             complement(3452..4396)
                     /locus_tag="PK754_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB08799.1"
                     /translation="MAVKRPTKAKKTKHVSPKNNSRSKKQPLIKTIANRKVKKTFPVS
                     INVPQIENLLSELEYGEELAEYYDLIYSEKVIHQIQSPFFQMLIKKYGIRSACDLSCR
                     SGQTLAALSKLGIKNLTGVDVSSKMLDLARKRCPSKTQFYQSELFLAPHTIGDETKFD
                     LVLCSRDALPLVLDDEGLLGFMAQARNLLSPNGILVIETWNYDKIWRRKERFMPVMDR
                     CSNKQSLLFFIENDFHGELLVHNVVKLEKNKFEWFLRTISTPIRPLTRNEMEFFVKEA
                     GFEKYGFLGNYAGSPFNTTESAYCIMLAMNNTNTITVS"
     gene            4549..5592
                     /locus_tag="PK754_04525"
     CDS             4549..5592
                     /locus_tag="PK754_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08800.1"
                     /translation="MRSQFIPILFSVLIACRGAGNNPQPTEQPKTFSIGDGKIVNSVQ
                     FSPDGKFLICGGENKHTKIIEFATGNVIWVSDIMPDAVLVNGFSPDGNRFFITTSDNT
                     QSTGSIGMYSFADRHRQWFIDKQTNDIQLARYSPDGRELIVGNFFNILFLDVETGKQT
                     KFFSGHPIEVPSPYGHYDAVTDIIFTSDPNVFVSVSWDKKILIWDMKAGHDVRTYAEG
                     DPINAMLLFPEGNRILTGSVDAITVWNRTNDAHEQTVVYDGEIKKMCWLEGGNYFVTG
                     DGDGDLTLWKTNGLERLKDISKAQERGIWSMSASPDGKAVVTGGGTGIVTWWPLEYLI
                     QYNAGNADSIKIK"
     gene            5620..5997
                     /locus_tag="PK754_04530"
     CDS             5620..5997
                     /locus_tag="PK754_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08801.1"
                     /translation="MKINRFLSFLVVSTCTILTFSGCDDKPNFPLNGTEWYGEFDGYV
                     NYLDFDKVVYVDAVKADGDTCFDIQFYQYTVDGDDFIIDGYITINWEVVKDTLTLTDD
                     VTSYKFIRTAIDDSSLAAQGCTD"
     gene            6123..8057
                     /gene="secD"
                     /locus_tag="PK754_04535"
     CDS             6123..8057
                     /gene="secD"
                     /locus_tag="PK754_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNB08802.1"
                     /translation="MIKSKNVRAVIIVICAALSLFALWPSFKFYNLSDAQKKEMLKTP
                     EGTKELNELQSKSINLGLDLKGGIHLVMETDVVTLYENIAKTKDAEFTRVFEKAAEEY
                     RKNPDAVFSNVLRTHFEDAKLSMPAYWGERFKDNADVVKFMDEQSKDAVDRAQQIIRN
                     RVDQFGVSEPTIQKQGDHRIIVELPGVEDPAQAKNLIQQTALLEFKLVKDPQDLGTAL
                     ERLDVYFAKVDTVSGKISAGAVSADTSKKSSDGGTGLSAELQKSSSDSSQKDSSALAN
                     LDKPLTKLLVSSQYDVLVPENNFEYVKSMLYYQVGERVKMKPGVQQVIGESNDIIFSS
                     KPIFEGHHALYLVKKQPELSGAVITEAKQDIYQGMDPGLAGRPIVTLKMTPEGSKRWA
                     IVTGANVGKRIAVVLDNKVQSAPNIQEKISGGNTQITGMENLIEAKSLAIALRAGSLP
                     APVHVMEERTIGPSLGADSIDSGKMAFWIGFVVIALAMVAYYRMSGAVADMALVINVL
                     LLFGVLTGFGFTLTLPGIAGIVLTMGMAVDANVLIYERIREEIRLGKTVRAAIETGFA
                     KAFVTIVDANLTTFGTGIVLFQFGTGPIKGFALTLMIGIVTTIFTGVFVSRFIMDLIY
                     DRSTIERQMSIGISYTKTSA"
     gene            8087..8977
                     /gene="secF"
                     /locus_tag="PK754_04540"
     CDS             8087..8977
                     /gene="secF"
                     /locus_tag="PK754_04540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNB08803.1"
                     /translation="MELFVNKNYPLVDTRRNAYIFSGLLILISLLLFFFRGLNYGVDF
                     VGGTSIEVKFSSKVELEKVRSIVSSKYSSDRIQDFGNGNEVLIHVLEQNNDVAPTVLK
                     LMNDNFPGNSVELRSVSQVGPKIGDELKVSAIWASIWGLLFIVVYLAIRFHWKWGMAA
                     VAALFHDVIITIGLFCALQLEFSLGAVAALLTIIGYSVNDTIVVFDRIRENTRLIKRG
                     TTFGEVINKSINETLSRTVMTSALTLLSVIVLLMIGGEVIRTFAWALLTGIVVGTYSS
                     IYVASPILIEWNAGEDIKVK"
     gene            9044..9382
                     /locus_tag="PK754_04545"
     CDS             9044..9382
                     /locus_tag="PK754_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNB08804.1"
                     /translation="MDYNIAENNGVVVLELKGNIMGGPDATQLNDEIHTRIKQGKKNF
                     VVNMQSVDLINSSGLGILISNLTTVKNNGGDLRLTNLSSKIKQIFQITKLATVFKQYD
                     SVDDGVKSFV"
     gene            9462..10736
                     /locus_tag="PK754_04550"
     CDS             9462..10736
                     /locus_tag="PK754_04550"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNB08805.1"
                     /translation="MSVRIVIGAQWGDEGKGKIVDLLASQAHVVARYQGGANAGHTIV
                     HGNKKYVLHLIPSGILHEGVMCILGNGVVIDPIALFDEIKMLSEMGIAMHDRLMISPH
                     AHIILPYHKLIDQAKEKYLGENQVGTTKRGIGPAYVDKIDRSGIRAIDLIHPDTLERK
                     LSKAITEKNKIIKAFGSEELPIEPTIKQFKEIGQQLKPLIGDSVKYLFESNQTGKEIL
                     LEGAQGTLLDIDHGTYPFVTSSNASAGGACTGLGIGPSYINHVMGVVKAYNTRVGNGP
                     FPTEFDEVFGQKVRELGGEFGATTGRPRRCGWLDLVQLKYAMMVNGIDELAITKLDVL
                     DTLEELKICVGYKVDGKRIDHYVTDAGELERVEPVYETVSGWKSDTSGVKNYVELPQK
                     AQAYLKFIEDYLGHARIKIVSIGQEREKTFMM"
     gene            10756..12210
                     /gene="gatB"
                     /locus_tag="PK754_04555"
     CDS             10756..12210
                     /gene="gatB"
                     /locus_tag="PK754_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNB08806.1"
                     /translation="MQYEPVMGLEVHAQLLTQSKAFCTCSTKYGAMPNTQTCPVCLAL
                     PGALPVLNKRAVDFAIRMGLATHCVIAEESIFARKNYFYPDLTKGYQISQFDKPLCEN
                     GWVDIEVGEAVKRIRIKRIHMEEDAGKSIHDDALTGGEKTFVDLNRCGTPLIEIVTEA
                     DFRTPAEVVAYLTKIRQTVQYLEICDGNMEEGSLRCDVNISMRPVGQEKFGTKTEIKN
                     MNSFKAVERALNYEMARQTDILQNGGSVSQQTLLWDDNQGVSRALRSKEEAHDYRYFP
                     EPDLVPLRVTKAWVDEIAGSLSELPGARRDRFVQQYGIPKYDADVLTLSKSLANYFEE
                     TLKHVSDGKLASNFIMGEVMALLKERKQDADAFIISPANLGKLLRMVSDNTISGKIAK
                     IVFEEMAKDGADAEAIVKTKGLLQITDRGEIEKIIDRMFELNQKQLQEYLGGKEAVFG
                     HFVGQVMKLSQGRTNPAMTNEILKQKLAEKKQNQ"
     gene            12304..12395
                     /locus_tag="PK754_04560"
     tRNA            12304..12395
                     /locus_tag="PK754_04560"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12338..12340,aa:Ser,seq:gga)
     gene            complement(12465..14441)
                     /locus_tag="PK754_04565"
     CDS             complement(12465..14441)
                     /locus_tag="PK754_04565"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HNB08807.1"
                     /translation="MGLGKRVLYALAVFIFQAYITGCAAGFQDIRQTQKDYGIEKLPS
                     AEEYPQHEAVIVLESHKIDVSIDNNFDVFTIEDVHVVKRIFRNVENHAEVEITLGYGE
                     KLKSIQARAITSTGQILELEKKEFLHSKGSSDGSVFYSDDRTTRFTFRGVDKGSIVEY
                     KYRIQKDYPFQMDVWYVQGSLPILRNEYKLFVPTILLDNNWNWFFKSYNYDIGLPKIE
                     KAGISANKTLDSKNTYTWLVQNVPAFEREPRMNSYYHHAGHVRFAPSYWNSWNAIADW
                     YYKKLFLPQLIVTPQISEKAQSLIANVADEKSKVQRLSDYVKKIRYTAIELGIGGIRP
                     NTPQTVYDRQYGDCKDKATLLIAMLKSVGITAHPVLVRTANSGFVDAQFPSWNFNHMI
                     VKIALSGGKTIWTDPTSETSQFGFLPWSDEDIDVFVLYPDGTGMIEHTPTSSHTNNLT
                     DVHIQGEAIGDMGMKFEIKIDFFGNDYSYYLYRLRDMSQNELENFCKNLIVDEYLNSK
                     ISNVRFSENDTAGKPLSLVFETTVEKVIQAQGDLYFANIDPFKLFESWSWLASEQRRF
                     PIRTIYPYLIKKRIEIKFPANLTIRNLPKPHNESIDYFSYNRSYQQAGNDALIFNETY
                     AVTERSVPAIEYNTVRKFFDKIKSGMAEKVILTK"
     gene            complement(14507..15211)
                     /gene="queC"
                     /locus_tag="PK754_04570"
     CDS             complement(14507..15211)
                     /gene="queC"
                     /locus_tag="PK754_04570"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNB08808.1"
                     /translation="MTTDTHSTLAVVLASGGLDSCVTTAIAAQTHALAMLHVNYGQRT
                     EKRELRSFNDICNYYSVLPSRRLIVNIEHLKQIGGSSLTDNSIPVSSADLHNKSIPSS
                     YVPFRNANILAMAVSWAETIKAEKIYIGAVQEDSSGYPDCRSDFFEAYNQMIRLGTKP
                     GSGIEIITPIIHLQKSEIVREGLHLNAPLSLTWSCYQSEDEACGVCDSCALRLRGFQS
                     AGIEDPILYRVRPKYD"
     gene            complement(15208..15642)
                     /locus_tag="PK754_04575"
     CDS             complement(15208..15642)
                     /locus_tag="PK754_04575"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNB08809.1"
                     /translation="MTKTVKIAKEFHWEMAHRLPYHTGGCQNIHGHSYILWVEIEGQP
                     QENGMLLDYAEIKRIVKPMIDMLDHGFICSRDDKAVLAFLNNSKFKMTIVDFYTTAEN
                     LCYYFADAIEKEIIKYPHVHSMKIRICETRSSYAEITRSLKR"
     gene            complement(15642..16322)
                     /locus_tag="PK754_04580"
     CDS             complement(15642..16322)
                     /locus_tag="PK754_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNB08810.1"
                     /translation="MQNTILRDTLKVNEIFYSIQGESTHAGKPCIFVRLTYCNLRCTY
                     CDTEYAFYSGRDMTVEDVIRTVQSYTCKTVEVTGGEPLLQERVYDLMIRLCDMGFEVM
                     IETGGSLDVEKIDPRVLKIVDMKCPSSGMQDKNYYPNLNKLLPTDELKFVIGDRNDYE
                     WAKALIAQYPHTKQINSILFSPVFDTIEPVSMVNWMLEDKLQTIVPNIRFQIQLHKVI
                     WSPETRGV"
BASE COUNT         4682 a         3236 c         3739 g         4782 t
ORIGIN      
        1 aacgacggtg tgaataacgg aattgatatc aatccgcaaa atgtgcaagc caaaacggtg
       61 atttcagtaa tgcgtcagat cggaaatcct tcgaatattg tccgtaatta tgaaaaagtt
      121 ttgagcatgt ccgagttcat gtcggaatcg gattacattc agttactcaa agaagccgat
      181 aaacagttgg ttgcttaccg tgatttgctt acggacaaac tcaacccatg aaagccaaaa
      241 cacttctgtt ggcccgaata aacttcatag gcatctattt tgcatgtttt caaacgacgc
      301 tatacgccgg tacttttgat tggcgtattg gcggcgagac tggtcctgtt atatcaaatc
      361 gaacggcaac cagtcgacaa tttttaatgg ctgcagcagg ttcagggcga tatcaatttg
      421 tgttcggaga acaccagctt acgtttcaag ggcgtggacg ttacgaagat tataatacgt
      481 cattcgaacg ccatgcatgg acttcgcatg ccagcaccga atacgtttat gcgagcggcg
      541 catgggtgtg gtcgttaaat actaattggc gcactcaaca gtatgggctt tcaggaccgg
      601 atgtacgcgc gactttgtat aatcttcaat cccgaggcga atattatttc ggacaaagga
      661 tgggtgcgtt tgcagaattg cgatttgaac gtaatatctc caaagtggat catgaaaatc
      721 gctttgcgtc ccgcgccggc gttattggat tagtatggtc cagcgcagaa tatttgaaag
      781 ttttcaccgg ttttcttacg gaacgttttt ttgcttccac gacggatcca ttgacttatc
      841 cgagtaaaaa taacggctgg cgcaacggta tggaaacagg gttggactac tcgggtacat
      901 ggattatcaa tggacgctat atcgcatcat ggcgtgtatc caaatatacc cttaattatt
      961 ctttagagca ggaagcgcat attgttatag gtaaacatat caatgaacgt tgggggttat
     1021 attttttagc cgattacgtt aggcgtgata taccgcgaga gaataaaatc actagaaatt
     1081 tgttatatac caattccgtt caggataatc gtgcgcacat caaactttca tatgaaacag
     1141 ccgccaatca acgattattc atacgagccg cttattcgca aacggaattt acccaaaaag
     1201 tagaaccttt ccgttacaaa accatagttt tgggatatga agttaacctc tgaacggata
     1261 cggtatctct tatttccacg tgtgttagac tatcgagagg acgatttttt taatcgcgtc
     1321 gctggtgatg ctgtgatgcg atttatgcca taggacttct ttgttttgaa tgacaaaaat
     1381 ctgcgggctt tgatgggtga tagcaaataa atcggcaacg cggttagaaa tcgggcggtg
     1441 cgtctgaacg acaatcatgc cgctgcgact attccattcc ggatattccg acaaaaaagt
     1501 ttctacttgt tccaaagcgt acgcgctagt cccgcagctg ttgctgtgtt tgaagagaag
     1561 caccgtagta tttaacgatt tttccagcaa agcatctaac atttccggcg attgaatctc
     1621 tgtcataatt ttaatctatt ttaaattaaa aataattacg tataaatgat aaacctgctt
     1681 gacaaacgcg ggatcgggtg tatatattcc gcagttcatg gcgcgttcgt ctaacggtta
     1741 ggacgtagcc ctctcaaggc tgtaatacgg gttcgattcc cgtacgcgct acacaaaagc
     1801 tcccttcatt tcgaagggag cttttatttt atctagtctt ctctcttttt aaaagtattc
     1861 ttaaaccaac tgcgatgatc tttgggctta ccggtttggt gttgggtttt ttgtttagtt
     1921 cttcgatccg atgaacccgt tttgtgttgt gggcgcccgt gttgtttagt ctgttgcggt
     1981 tttataccat gatcatgtaa ggtgttagcc gcagcttttg cgggtgtcac agcgatcggc
     2041 atatccataa ccgggatagt ttttccgatt aatttctgta tatctttcaa ataggcgcgt
     2101 tcctccggat cacaaaaaga aatggctgta ccgctcatac cggctcgtcc cgtgcgaccg
     2161 attcggtgaa cataggtttc tggtatatta gggatttcgt aatttatcac atgggataaa
     2221 ttatccacat caatcccgcg cgcagcgata tctgtagcta ccagaacgcg ggttttagca
     2281 tttttaaaat ttcccaaagc acgttgacgg gcgttttggg atttattgcc atgaatagct
     2341 tccgctgtaa caccggcttt gagcaaatcc cgcgttactt tatcggctcc gtgtttggtt
     2401 cgtgtaaaaa tcagagctga agtaatctca ggatttcgca aaacatgaat aagtaaattt
     2461 tttttatccg gtttattgac aaaataaacc gcttgttcga ttttttcagc tgtggatgac
     2521 accggcgtga cggagacttg ttcgggatgt gataaaattg aatgtgccaa cttttgaatt
     2581 tcagcaggca tcgtggccga aaaaaggagc gtctggcgtt tggaaggtaa cttaccgaca
     2641 atctttttta catcatgtat aaagcccatg tccaacatgc gatctgcttc atctaataca
     2701 aaaatcttaa gttgttgaag gtggacgaac ccctgctgca tcagatccag taatcggccc
     2761 ggggtggcga taaggatatc gacgccgact ttcaatgcct gagtttgagc atgttgcgat
     2821 acaccgccaa aaatcacggc atgtcgcaat cccgtatgac gaccatacgc cgtaaaactt
     2881 tcaccgattt gaattgccag ttcacgcgtc ggagttaaaa taagcgcgtg ggttttacgc
     2941 gggatatgaa ccgatgacga ttgaaataag agttgcaata tcggaatcgc gaatgcggct
     3001 gtttttcccg tacccgtttg tgcgcagccg agcaaatctt tgtgattcaa aatcagaggt
     3061 atagcttgtt cctgaatagg ggttggtttg gtataaccct ccgatttgag tgcttgtaaa
     3121 ataggttcga tgacatgaag ttgcttgaaa gccatacatc attctttctt aaaaagtgct
     3181 ttacggatga gatcctaaag cgataaaaaa tgccaacgaa atatacggcg tgaaaaactc
     3241 ggacgcacga atacctcgtt aaaaaattaa attcgattat gatacggaag taagaggccg
     3301 agacctgaac tttgagccac ttttgaagcg atcacaaatc cggtagcgga attgaaggcg
     3361 gaaaagaaac tcatgatcat agatcgtagt ggggcagtat aacttaatat atagtcccgc
     3421 gcaagagtta ttaacaaatt caaaaaaacg gttatgatac cgttatggtg tttgtattat
     3481 tcatcgcaag catgatacaa tacgcactct ctgtcgtgtt aaatggcgag cctgcgtagt
     3541 tacccaaaaa accgtatttt tcaaaccctg cttcttttac gaagaactcc atttcgttgc
     3601 gcgtgagcgg gcgaatgggt gttgaaatgg ttcgtaagaa ccattcaaat ttgttttttt
     3661 cgagtttgac aacattatgc accaacaact cgccgtgaaa atcgttttcg ataaaaaaca
     3721 gcagagattg tttattactg cagcgatcca tcacaggcat aaaacgttct tttcggcgcc
     3781 agattttatc gtaattccat gtttctatga caagaatacc gttgggtgaa agcagattac
     3841 gcgcctgcgc cataaaccct agaaggcctt cgtcatccag aaccaaaggc aatgcatcgc
     3901 gcgaacataa aacaagatca aacttggttt catcgccgat ggtgtgcgga gcgagaaata
     3961 actcactttg ataaaattgt gttttagagg ggcaacgctt ccgagcaaga tcaagcattt
     4021 tggagcttac gtcaacaccg gttagatttt tgatgcccag tttggatagc gcagcaagcg
     4081 tttgaccgga acggcaagat aaatcgcaag ccgaccgaat gccgtatttt ttgattaaca
     4141 tttgaaaaaa aggagattga atttggtgta ttaccttttc tgaataaatc agatcgtaat
     4201 attcagccag ttcttcgccg tattcaagtt cggacaaaag gttttcgatc tggggcacat
     4261 tgatgctgac gggaaaagtc tttttgactt tacgattagc tatagtttta atcagtggtt
     4321 gttttttaga gcgcgagttg tttttagggg aaacatgttt tgtttttttt gcctttgtcg
     4381 gtcgtttaac ggccatattt aacctttaaa aatgatagcg tggtattcaa aaaatagaac
     4441 gtgagtaatt aagattctac gcttgattct caagtttgaa cgggctaatt tagtgccctt
     4501 tttagacaat taccacgaca aaatcgtaaa ccattgtgga aaaaatatat gcgaagccag
     4561 tttattccca ttctcttttc agtgttgatt gcctgccgtg gcgctggaaa taatcctcaa
     4621 ccgacagaac aacccaaaac attttctatc ggtgacggta aaatcgtcaa ttccgtacaa
     4681 ttttcaccgg atggcaagtt tctgatctgt ggcggagaaa acaagcacac gaaaattata
     4741 gaatttgcaa ccggaaatgt aatatgggta tcggacataa tgccggatgc cgttttagta
     4801 aacgggtttt cgccggatgg gaatcgattt tttattacaa caagtgacaa tacgcaatcc
     4861 accggaagta tcgggatgta ttctttcgct gaccgccatc gccagtggtt tattgacaaa
     4921 caaacgaatg atattcagct tgccagatat tcgccggatg gtagagaatt gattgtcggt
     4981 aattttttta atatcctttt tttggatgtg gaaacaggaa aacaaactaa atttttttct
     5041 ggccatccca tcgaggttcc gtccccgtac ggacattatg atgcggttac agatataatc
     5101 tttacgtcag accccaatgt atttgtgtcg gtaagctggg ataaaaaaat tttaatttgg
     5161 gatatgaaag cggggcatga tgttcgtacc tacgctgaag gtgatcctat caatgcgatg
     5221 ttactatttc ctgaagggaa tcggatactc acaggcagtg tagacgccat tacagtttgg
     5281 aatcgaacaa acgacgcgca cgaacaaacg gtcgtttatg atggtgaaat taaaaaaatg
     5341 tgttggcttg agggtggaaa ctattttgtg accggtgacg gggatggtga tctgacattg
     5401 tggaaaacca atggtttgga aagactaaaa gatattagta aagcacaaga gcgaggaata
     5461 tggtctatgt cggcttctcc ggacggtaaa gccgttgtta cgggcggcgg caccggcatt
     5521 gtcacttggt ggccgttgga atatttaatt cagtataatg caggaaacgc agattctata
     5581 aaaataaaat aaagcgcttt gtcaaaaaaa ggggacgata tgaagattaa tagattttta
     5641 tcttttttgg ttgttagtac atgcacgata ttgactttct ccggttgcga cgacaaacct
     5701 aattttccac ttaatggtac ggagtggtat ggagagtttg acggttatgt taattattta
     5761 gactttgata aagtagtgta tgttgatgcc gtcaaagcgg atggcgatac ttgctttgat
     5821 attcaatttt atcaatatac cgttgatggt gatgacttta ttatcgatgg ctacattacg
     5881 ataaactggg aagttgtaaa ggatacattg acgttaactg acgatgtgac atcgtataaa
     5941 ttcatacgca cggcgattga tgattcgtca ttagcggcgc aaggttgtac ggattagatc
     6001 aggcattttc cttcattttt aataaacttg acattatgaa tacggccatc tatattaggc
     6061 gcgttttttt caaaactgct gataattaaa tacttaatag atttatcgga ggatatgaac
     6121 gaatgattaa gagtaaaaat gtcagagcgg tgatcattgt gatctgtgca gccttatcgc
     6181 tgttcgcgct ttggccttca ttcaaatttt ataatttgtc tgatgcacag aaaaaagaaa
     6241 tgctcaaaac acccgaaggc acgaaagagc ttaatgaact gcaatctaaa tctatcaatt
     6301 taggccttga cctcaaaggc ggtatacatc ttgtgatgga aacggatgtg gtgacgctct
     6361 atgaaaatat agccaaaacc aaagatgccg agttcacacg ggtttttgaa aaagcggctg
     6421 aagaatatcg taaaaacccg gatgctgttt tttccaatgt tttacgtact cattttgaag
     6481 atgcaaaatt atctatgccg gcctattggg gtgaacgctt caaggacaat gcggacgtgg
     6541 ttaagtttat ggatgaacaa tccaaagatg ccgtggacag agcgcagcag atcattcgta
     6601 atcgcgttga tcaatttggc gtgagcgaac ccactattca aaaacaaggc gaccatcgta
     6661 taatcgtgga attaccgggc gtcgaagatc cggctcaagc taaaaatctg atccagcaaa
     6721 cggcgttgct tgaatttaag ctagtcaaag acccgcagga tttgggtaca gcgttggaac
     6781 gcttggatgt gtattttgca aaagtagata cggttagcgg caaaatcagc gccggtgccg
     6841 tttcggccga tacgtcgaaa aaatcatcgg atggcggtac agggttgtcg gcggaacttc
     6901 aaaagtcttc ttccgattcc tcacaaaaag attcttccgc gttagctaat ctggataaac
     6961 cattaacgaa acttttggtt tcgtctcaat atgatgtatt ggttcccgaa aataattttg
     7021 aatacgttaa atccatgctc tattatcagg tcggcgagcg tgtcaaaatg aagccgggtg
     7081 tacaacaggt gatcggtgag tccaatgata tcatcttcag ttctaagcct atttttgaag
     7141 gccaccacgc gttgtatttg gtgaaaaaac aacccgaatt atcgggcgcc gtgatcaccg
     7201 aagcgaaaca ggatatttat cagggtatgg atccgggatt ggccggacgc ccgatcgtta
     7261 cgcttaaaat gacacccgaa ggcagcaaac ggtgggcgat cgtgaccggt gctaatgtcg
     7321 gcaaacgcat cgccgttgtt cttgataaca aagttcaatc cgccccgaat attcaggaaa
     7381 aaatttcagg cggtaataca caaatcacag gcatggaaaa ccttatcgaa gccaagagcc
     7441 ttgctatcgc actgcgtgca ggttcacttc ccgcgccggt tcacgtgatg gaagaacgta
     7501 cgatcggccc ttcgttggga gcggattcga ttgacagcgg aaaaatggca ttctggatag
     7561 gcttcgtggt tatcgcttta gcgatggtgg cttattaccg tatgtccggc gctgtggcgg
     7621 atatggcgct tgtaatcaac gttctattac ttttcggagt tctcaccggt ttcggtttta
     7681 cgcttaccct gcctggtatt gcaggtatcg tgcttacgat gggtatggcg gttgatgcca
     7741 acgtgctaat ttatgaacgt attcgtgaag aaattcgcct cggaaaaaca gtgcgtgcgg
     7801 cgattgaaac cggttttgca aaagcttttg ttacgatcgt agatgccaat cttacaacat
     7861 ttggtacagg tatagtgttg ttccaatttg gtaccggtcc gatcaaaggt tttgctttga
     7921 cgctgatgat cggtatcgtc accacgatct tcaccggtgt gtttgtgtcg cgctttatca
     7981 tggatctcat ttatgatcgt tctacgattg agcgtcagat gagtatcggt atttcctata
     8041 ccaaaaccag cgcataaaat aacgaaattt tcgaggagca tagataatgg aattgtttgt
     8101 aaataaaaat tacccgcttg tcgatacgcg tcgcaatgcg tacatttttt cgggtttact
     8161 cattttaatc agcttgttgt tattcttttt ccgaggactt aattacggtg ttgacttcgt
     8221 cggtggtacc tccatcgaag ttaagtttag ctccaaagtg gagttggaaa aagttcgaag
     8281 catcgtttca tcaaagtata gctctgaccg tattcaagac ttcggtaatg gaaatgaagt
     8341 gttaatacac gttttggaac aaaataacga tgtagctccg accgttttaa agctgatgaa
     8401 cgataacttt cccggcaatt cggttgaatt acgttcggtg agtcaggttg gccccaagat
     8461 cggtgatgaa cttaaagttt ccgccatctg ggcttcgatt tggggacttc tgttcattgt
     8521 tgtatacctt gcgattcgtt ttcattggaa atggggcatg gctgccgtgg cggctttgtt
     8581 ccatgatgtg atcatcacca tcggtctatt ttgtgcgttg caacttgagt tttcgctggg
     8641 tgccgttgcc gctttgctga ccatcattgg ttactccgta aacgatacca tagtcgtatt
     8701 tgatcgtatt cgtgagaata cgcgccttat caaacgcggt acgacctttg gagaagtcat
     8761 caacaaaagt attaatgaaa ctctgagccg tacggtgatg acctcggcat taaccttgtt
     8821 gtcggttatc gtattgctga tgatcggcgg tgaggttatt cgcacatttg catgggctct
     8881 gttgaccggt attgtcgttg gaacgtattc atccatttac gtggcgagcc cgatcctgat
     8941 cgaatggaat gccggagaag atattaaagt caagtaataa tctgcgcttg atcgtgatgc
     9001 ttttttggtt tatgttacgc cagtcaattt agaggttacc gctatggatt ataatattgc
     9061 agaaaataac ggtgttgtcg tattggaatt aaaaggcaac atcatgggcg gtccggatgc
     9121 aacacagctt aatgatgaaa ttcatacccg cataaagcaa ggaaaaaaga attttgtcgt
     9181 taatatgcaa tccgttgacc ttatcaatag ctcaggactg ggtattctga tcagcaatct
     9241 gactacggta aaaaataacg gcggtgattt acgcttgacc aatctgtctt cgaaaatcaa
     9301 acagatattt caaataacaa aattggcgac cgtatttaag caatacgatt ctgtggatga
     9361 cggggttaaa agttttgtct aaaaattaaa ttatttttaa acataataac ccaagatctg
     9421 aaacagattt tgggttattt tttgtctgat cggaaaaaac tatgtctgtt cgaattgtta
     9481 tcggcgccca atggggagat gaaggtaaag ggaaaattgt tgatttatta gccagtcagg
     9541 cacatgtcgt agcgcgttat caaggcggcg ctaatgcagg gcataccatc gttcatggaa
     9601 ataaaaaata cgttttgcat ctgataccct caggtatatt gcatgagggc gttatgtgta
     9661 ttctcggcaa cggtgtagtg atagatccta ttgcgctttt tgacgagata aaaatgctgt
     9721 ctgaaatggg gatcgcgatg cacgatcgtt tgatgatcag tccgcatgcg catattattt
     9781 tgccgtacca taaactcatt gaccaagcta aagagaagta ccttggtgaa aatcaggtcg
     9841 gtacgactaa gcgaggtatt ggaccggcgt atgtggataa aattgatcgc agcggtattc
     9901 gtgcgattga tttgattcat ccggacacgt tggagcgaaa actttccaag gctattacgg
     9961 aaaaaaataa aataataaaa gcatttggct ccgaagaatt accgatcgag ccgacgatca
    10021 aacaatttaa agaaatcggc cagcagctca agccgctgat cggtgacagt gtcaaatatc
    10081 tttttgagtc caatcaaacc ggaaaagaga ttttactcga aggcgcccaa ggtaccttgc
    10141 tggatattga tcacgggaca tatccttttg tgacttcatc caatgccagc gccggaggcg
    10201 cttgtacggg attgggtata ggtccttcct atatcaacca cgttatgggt gtagtcaagg
    10261 cttacaatac gcgtgtcggt aacgggcctt ttccaacaga atttgatgaa gtttttggtc
    10321 aaaaggtgcg tgaactcggc ggagaatttg gcgctacgac cggcagaccg cgtcgttgcg
    10381 gatggttgga tctggtgcaa ctcaaatatg ctatgatggt taacggcatt gatgaattag
    10441 ccattaccaa gttggatgtg ctggacactt tagaggagct gaaaatatgt gtcggctata
    10501 aggtggatgg taaacgcatt gatcattatg tgacggatgc cggtgagttg gaacgtgtag
    10561 agcctgtgta tgaaaccgtt tctggatgga agtccgacac atccggcgta aaaaattatg
    10621 ttgagttgcc gcaaaaagca caagcgtatc tcaaatttat cgaagattat cttggccatg
    10681 cacgtatcaa gatcgtttct atcggtcagg aacgcgaaaa aacatttatg atgtaagagg
    10741 aatagtatgt ccgttataca atatgagccg gtaatgggtc tggaagttca tgctcagctt
    10801 cttacccaat ccaaagcgtt ttgtacctgc agcaccaaat acggtgcgat gcccaatacg
    10861 caaacttgtc cggtctgtct ggcattgccc ggcgcgttac cggtgctcaa taaaagagca
    10921 gtggattttg ccattcgtat gggattggct acgcattgtg ttatcgccga agaaagtatt
    10981 tttgctcgaa aaaattattt ttatccggat ctgactaaag gttatcaaat atcccaattt
    11041 gacaaacctt tatgtgaaaa cgggtgggtg gatattgaag tcggcgaagc cgtaaagcgg
    11101 attcgtataa aacgcataca tatggaagaa gatgcgggca aatctatcca cgacgatgcc
    11161 ctcaccggcg gcgaaaaaac ctttgttgat cttaaccgct gcggtacccc cttgatagag
    11221 attgtaacgg aagcggactt tcggactccg gcagaggttg tcgcatattt gaccaaaatt
    11281 cgccagactg tacagtattt ggaaatttgt gatggaaata tggaagaagg cagcttgcgg
    11341 tgtgatgtca atatatccat gcgtcccgta ggtcaggaga aatttggcac caaaacggaa
    11401 attaaaaaca tgaattcatt caaagcggta gaacgcgctt tgaattacga aatggctcgt
    11461 caaacagata ttctgcaaaa cggcggtagt gtcagtcaac agacgttgtt gtgggacgac
    11521 aatcaaggtg tttcacgcgc attgcggtcc aaagaagaag cgcatgacta tcgctatttt
    11581 cccgagccgg atctggtacc tttacgtgtt actaaagcct gggtggatga aatcgccggt
    11641 agtttgtccg agttacccgg agcgcgtcgg gatcggtttg ttcaacaata cggcattcct
    11701 aagtatgatg ccgacgtttt gacgctcagt aaatcattag ccaattattt tgaagaaacg
    11761 ctaaaacacg taagcgacgg caagttggcc agtaatttta ttatgggcga ggtcatggct
    11821 ttgttgaagg agcgcaaaca ggatgccgat gcctttatca tttcaccggc caatcttggg
    11881 aaattacttc gtatggtatc cgataatacg atcagcggaa aaattgcgaa gatagtattt
    11941 gaagaaatgg caaaggacgg tgcggatgcc gaagctatag tgaaaacaaa aggtttgctc
    12001 caaatcacgg accgcggaga gattgaaaaa attattgatc gtatgtttga attgaatcaa
    12061 aaacaattgc aggaatatct tggcggaaaa gaagccgtgt ttggtcattt tgtcggtcaa
    12121 gtgatgaagc tgtcgcaagg aaggaccaat ccggccatga cgaatgaaat tttgaagcaa
    12181 aaactcgctg aaaaaaagca aaatcaataa attccttgca ttattgagcg tgcttgtcta
    12241 tttttcgcgc gcataaaaat attttcatat taagcgtttg tttaatatga ggccaagagc
    12301 cacggagagg tgcaggagtg gtttaactgt cacgcctgga aagcgtgtgt acccccaaaa
    12361 gggtaccgtg agttcgaatc tcaccctctc cgccaacaaa aaaagccgat tttaagtcgg
    12421 cttttttgtt ttaggatatc aaatgttttt attatcaaat gctcttattt tgtaagtatt
    12481 accttctcag ccatacctga tttaatttta tcaaagaact ttcgaacggt attatattct
    12541 atggcaggaa cggatcgttc cgtgacggcg tatgtttcat taaaaattaa cgcatcatta
    12601 ccggcttgtt gataagatcg gttatagcta aaatagtcta tggactcatt atgaggtttt
    12661 ggcaagtttc gtatagtaag attggcggga aatttaattt ctatccgttt cttaattaga
    12721 taaggataaa ttgtacgaat ggggaaacga cgttgttccg aagcaagcca gctccaactt
    12781 tcaaacaact taaacgggtc aatattggca aaatataaat caccttgcgc ttgaatgact
    12841 ttttcaacgg tcgtttcaaa aacaagagag agcggttttc cggccgtgtc gttttcacta
    12901 aaacgaacat tacttatttt ggaatttaaa tattcatcaa caattaagtt tttacaaaaa
    12961 ttttcaagtt cattttggga catatcccta agacgataca aataatagct gtaatcattg
    13021 ccaaaaaaat caattttaat ttcaaatttc attcccatat cgccgatagc ttcaccctga
    13081 atatgtacat cggttaaatt atttgtatgt gacgaagtgg gtgtatgttc aatcataccg
    13141 gtaccgtcgg ggtacagcac aaatacatca atatcttcat cagaccacgg aagaaatccg
    13201 aactgactgg tttcggatgt aggatctgtc caaattgttt ttcctccgga caacgctatt
    13261 ttgacgatca tgtggttaaa attccaactc ggaaactgag catccacaaa accggaattg
    13321 gctgttctta caagtaccgg atgggccgta atcccgacag atttgagcat tgcgatcaat
    13381 aacgtggctt tatccttaca atcaccgtat tgacgatcat agacggtttg tggcgtatta
    13441 ggacgaatgc caccaatgcc taattcaatg gcggtgtatc ttattttttt tacataatcg
    13501 gacaggcgtt gaacctttga cttttcatcc gcgacgtttg ctatcaaaga ttgggctttt
    13561 tcagagattt gaggtgttac aatcaattga ggtagaaata gttttttgta ataccagtcg
    13621 gctatagcat tccaactgtt ccagtaactt ggagcaaatc gtacgtggcc ggcatgatga
    13681 tagtaggagt tcatacgcgg ttcccgttca aacgcaggta cgttttgaac taaccatgtg
    13741 taggtatttt tgctgtctaa agttttattt gcagaaatgc cggctttttc aatcttcggt
    13801 aaaccaatat cgtagttata agatttaaaa aaccaattcc agttattatc taacaagatg
    13861 gtgggcacga agagcttata ttcgttgcgt aaaatcggca aacttccttg aacataccat
    13921 acatccattt gaaaagggta atccttttgt atgcggtatt tatattcgac gatggagccc
    13981 ttgtcaacac cgcggaacgt aaatcgtgtc gtgcgatcat cgctataaaa tactgaaccg
    14041 tcggacgagc ctttggaatg cagaaattct tttttctcca gctctaaaat ttgccctgta
    14101 gaagtaatgg cccttgcttg gatagatttt aatttttctc catagcccaa agtaatttct
    14161 acttcagcgt gattctcgac gttgcgaaat atacgtttga caacgtgaac atcttcaata
    14221 gtgaagacat caaagttatt atcaatactt acatcaattt tatgagattc aagcacgata
    14281 acggcttcgt gttgagggta ttcctcagcg gacggtagtt tttcgatgcc ataatccttt
    14341 tgtgtttggc ggatatcctg aaaaccggcc gcacatccag taatgtaagc ttgaaaaata
    14401 aaaacagcta acgcatagag aaccctttta cctaagccca taacaaactc cctcaattaa
    14461 attaataaga gactgatact acgaaaatcg aatgcaaaca gatcaactaa tcatattttg
    14521 gtcttacccg atataagatc ggatcttcaa tgcccgcaga ttgaaatccc cgtaatcgta
    14581 atgcacaact gtcacaaact ccacacgcct catcttccga ttggtagcac gaccatgtaa
    14641 gcgataacgg ggcattcaga tgcagtccct cgcggacaat ttccgacttt tgtaaatgaa
    14701 tgattggggt gatgatttca attcccgaac ccggtttggt gcccaaccga atcatttgat
    14761 tgtatgcttc aaaaaaatcc gatcggcaat cagggtaacc gctggaatct tcctgtacgg
    14821 cgcctatata aattttttcg gctttgatcg tctcggccca acttacggcc atggccaaaa
    14881 tattggcatt gcgaaaaggc acataagaac tggggatgct tttattatga agatcagccg
    14941 atgagacagg aatcgaatta tcggtcagac tggaaccgcc aatttgtttt agatgttcga
    15001 tattaacgat caagcgtcgt gagggtagaa cactgtaata attgcaaata tcgttaaacg
    15061 agcgcagttc gcgtttttct gtacgctgac catagttgac atgcaacatt gcaagcgcgt
    15121 gagtttgtgc cgcaatagcc gtcgtgacac agctgtcaag tccgccgctt gcgaggacga
    15181 cggccaatgt agaatgggta tccgttgtca tcgtttcagg gatcgggtta tttcggcata
    15241 cgacgaacgc gtctcgcaaa ttctaatttt catggaatga acatgaggat attttattat
    15301 ttctttttcg atggcgtccg caaaataata acacaaattc tcagcggtcg tataaaaatc
    15361 aacgatagtc attttgaatt ttgagttgtt cagaaacgca agaactgcct tatcgtcacg
    15421 actgcatata aaaccatggt cgagcatgtc aatcatcggt ttcacaattc gtttaatttc
    15481 tgcatagtca agtaacatac cgttttcctg aggttgtcct tcgatttcaa cccatagaat
    15541 ataagagtgg ccgtgaatat tctgacaacc gcccgtatga taaggcaggc gatgggccat
    15601 ttcccagtga aattcttttg caattttgac ggtttttgtc attatacacc acgtgtttcc
    15661 ggtgaccata tcaccttgtg taactgtatc tgaaaacgaa tattaggcac tatcgtttga
    15721 agtttatctt ccagcatcca gttgaccata gaaaccggtt cgatagtgtc aaataccggc
    15781 gaaaaaagta tagaattaat ttgtttggta tgcggatatt gagcaatcaa agccttcgcc
    15841 cattcataat cattacgatc gccgataaca aattttaact catccgtcgg taatagtttg
    15901 ttcaggttag ggtaataatt tttatcctgc ataccggaag aggggcactt catatcaacg
    15961 attttcagta cacggggatc gattttttca acatcgagac tgccaccggt ctcgatcatt
    16021 acctcaaatc ccatatcgca gagccgtatc atcaaatcgt aaacacgttc ttgcaaaaga
    16081 ggttcgccac cggtcacctc aacggtttta caggtatatg attgaactgt tcgaatgaca
    16141 tcttcaacgg tcatatcccg cccgctataa aaagcgtatt ctgtatcaca atatgtgcag
    16201 cgcaggttac agtacgtcaa acgcacaaat atacacggct taccggcgtg cgtagattcg
    16261 ccctggatgc tgtagaaaat ttcgttgacc ttaagcgtgt cgcgaagtat agtattttgc
    16321 atggcggcgc aatatatcaa aagaggctat acggcgcaag gcgaaaggta tttttatggg
    16381 ttgaatttat gcatcaagtg ccatacattc ttgccattca aacgaacgca ttatgttac
//
LOCUS       DAOQNQ010000042        16138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1279_length_16138_cov_5.680843, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000042 DAOQNQ010000000
VERSION     DAOQNQ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16138
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1279_length_16138_cov_5.680843"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..983
                     /locus_tag="PK754_04585"
     CDS             78..983
                     /locus_tag="PK754_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08811.1"
                     /translation="MSKSVALIVTIALLAIGCDKDGAIVKSDVGSDQTLIISHDPNKG
                     GTIGAVLPPPDSMLYVFIGNTDTSSSSTSKYFMFLEHDLNTNINGRTLKKYLDYGTLN
                     VGGFAAQRYHTYYVVNVPDSGGIPAHKDTVDATRYDGFPLTLPLDPVNNKLTISGSNS
                     THINNFSTDISVPTQLKFINLTFNQTIGDNEDLHLKLNRVSPAGFVGFVPQNIKFGDS
                     TSVGYIYSFFDYTVPTDEVVISKQHLEQLISKWNQDEVIYHLFVFEENVVGSINTIDK
                     TNGQSYGLPVEIDYTHNVLVKLKKQ"
     gene            1030..3201
                     /locus_tag="PK754_04590"
     CDS             1030..3201
                     /locus_tag="PK754_04590"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNB08812.1"
                     /translation="MKKIVACLSLILFCNSLLTAQERPVIRIAIGKHDVEHLGGWPLA
                     RRWYAIAAQHAKQNGAQAIYFDMAFPSADALHPESDEVFYHECVRDDKIFLMTERVQE
                     NNTILGGQSFPAGRTFLPFAAHWQIENAYLIYTPGDHSLVSALTEVPEETVILHWPSD
                     TLLPDMHLSAFLSSNSDLSNRSVVFYFDYPGVTSYVASPQGSVNPVSLIQIWAAEEIK
                     QGRMWKQWPLWLLVVLFAGTAVVSFLSLRDTRWIWIGAPALIWCAFAAGLYFLRIYLP
                     IGIGWTLVPILVSTFMLGIKRRTVTVTKEVASAASDDLTDLRYRLNFYEKISDQIRPL
                     DLSECREAQGIYYHKDSPVAELLQKADQVAQSNVPVLITGESGTGKERIAEFIHRRSQ
                     RASGPFVAVNCGAFNDNLIEAELFGYEKGAFTGAVQAKPGRFEMADNGTLFLDEIAET
                     SPAFQVKLLRVLQEGRFERVGGVVSKSVSVRVIAATHRDISVLIRDKQFREDLYYRIA
                     GFVLPLKPLRERTVDIDFLFRQFVYEIAPELKYSDHLVQWLCTLAWPGNVRELKAATQ
                     RSVINARMRHRNYILPADFEIRGATFSDSEQSLAERVLHALRDHQFKHRAMSAVADDL
                     GLHRITVTEYFRGWVIKAMIRCEQEPQAMFEYLRGTSDISEPKALKQRIDEYQNNISE
                     RIRTGVKKNESPEEVIRLRFRNTPKFFYDDLIQLIKEYRHD"
     gene            3194..5164
                     /locus_tag="PK754_04595"
     CDS             3194..5164
                     /locus_tag="PK754_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08813.1"
                     /translation="MTRWGLLMLVFVSASLFAQNDADAYRPMRYDARLTFVSEYTARQ
                     FASKEYVASRIHYMHNHGLYGFSVLVNMNQQLPFTIMPGTTTLVLPDSVKLVDRSQIT
                     NHAHTYVVDLPAALELRRRFARFEIAFGSQILVEKEEIVSTLVKELTSKVYYVDYEFA
                     SHRRSSLLGYGAMTSYWGPFAVTTGILPWRMIRMGNDSMHYALKNKLSGFLDVQYRYR
                     SHSMTTRVGRDAWGGNVQLKYDALQVNNEYCRSLRPSDYQVWKLNLSYDLSGGFTLLA
                     GYESTWYLAENFRSQNFRQWLNRHGDISSGKLTNQLPHKTVQIGLRYDLSGSRAADYV
                     KVVNMKIFQTKVYTAKRAYYAYNPVGQMDVHNASDEHLNVVIHAEAGAWGEIFRSSSI
                     DLAPHEIKSVPLFFYIPDQSVEQSGMVAQVKITGEVRGKHIALSSFPVTLYDIHSWDG
                     DTWGLRYFIAPDDPEIQSFAKSNLLTIGTDSAYSELYNKFHTLKKFVTAVGKNLRYVS
                     DPTTTFVNERVQYPSETLRQKSGDCEDLVAFMATNLMAIGHACAVVDIRPTLTENINI
                     PTAKPGSIGHVFLLVDTSIPVNAVDQLGLSEFEYVARPDKTGRVTIWLPLETTVVNGG
                     FEHAHREGVRQYYDEVIQKNGVVHGTVHVYDF"
     gene            5136..5846
                     /locus_tag="PK754_04600"
     CDS             5136..5846
                     /locus_tag="PK754_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08814.1"
                     /translation="MERYTYMIFKTICYILLTSPLMGQSEIWLKLEKGDAMYLDVTSM
                     KWQPLGSKEKIAPRTYVLTKADATLKIYRETNVYDAPAGGYFYIEDMFVNNKRDLVSE
                     ITRIEAEQLPSQPVERANDQKFGLTYGRANETLAENQSVPYLSERMRAIRWFEEHQYA
                     ETALLSLKRLISKFPSLYFEPSNFDRLCSLYMKLGLYGFLFDETNRFMTLKRDDEFGQ
                     MVIRWNETAKKKLTTSGQ"
     gene            6080..7129
                     /locus_tag="PK754_04605"
     CDS             6080..7129
                     /locus_tag="PK754_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08815.1"
                     /translation="MDSTIWDMQGQSGFVDYQGRKSIFLNGGAAVLKNLELRDGVMDV
                     DVSTPAARGFFGIQFRISEDGLNGEWVYLRQHKSGLADAMQYTPILNTGLNWQLYSGP
                     GFIGAVDIPKNEWFHLRLEVSGAQARLFVKNMDKPVLVMDDLKSGIQKGSIALAVLTG
                     ATYFSNFEVKKTTDAPWVRKHPAMLPNVLKQWEISPSMDALARNVESPLTQAEKKSIV
                     WQSVETEAPGMVTLSRYREAPHLRVSFANDFSKRLEAQPGSKVVYAKTKITSDRDQIK
                     KLYIGYSDEVCVFLNGQILYRGRSAQYFRDPGFLGIMDSENDALYIPLKKGDNELILA
                     VSELGGGWGFICRLE"
     gene            complement(7143..8015)
                     /locus_tag="PK754_04610"
     CDS             complement(7143..8015)
                     /locus_tag="PK754_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08816.1"
                     /translation="MKIRIRIGGFLLLWYIPRLTTQQIFLMIGVVLSVLINLAAIFFI
                     EIVVNPTKPPQLISDSEKAVLLKLLEEPDMSKVRDLLEQPKANDKVAQKPDALSDRNS
                     TTDVKPPDEIKNFGSVDVTQNQEPRPKTENTTPPDESTDEDRFQNNMNVQPFLKRNTN
                     QKSVYERLTGQEDPSQQGFAGQNYELNTYKWEFAPYMLAWKNKMTSNWYRITSKIFFS
                     QFARVGEILIYVKMNRQGQLVDSKVVDYNCDKSFVAPAYASVVNSFPLDPLPNDFPED
                     MLETTWTISITNQN"
     gene            complement(8012..9682)
                     /locus_tag="PK754_04615"
     CDS             complement(8012..9682)
                     /locus_tag="PK754_04615"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNB08817.1"
                     /translation="MHRYILIIILMCLSACGTRANIDMLVVNGKFVTMDDELNNNEIE
                     SIAIRDGRIVDMGKAEVMKKKYVTNRIEDVKGQLVLPGLIDAHLHMLNIGHYLTQLDL
                     VGTESYDQVITLVRDRVANTNKGAWIRGRGWDQNDWSKKDFPEHAALTAVSPDHFIVL
                     ERVDGHAVLVNQNVLTAAKINRETPDPRGGKIMRNAQGEPTGVLIDNAIDLLIPYIPK
                     YTLSEDSLALEAAMSACLKVGLTSVHDAGVDTNHIDVYKELGRRNKLCVRVYAMLNGF
                     DRRLLDMYYKKGPEKNLFNQFLTIASIKLYSDGALGSRGAALLEPYSDSPQSKGLELM
                     MKPRLEIIATDALKWGFQVCTHAIGDRGNRNTLDAYEAAMKAVPDSATYKRFRIEHAQ
                     VVNEADIPRFATLGVIASMQPTHCTSDMYWAEERVGKDRIRGAYAWQKFIKNGVRLAA
                     GSDAPVESHNPLLGVYAAVTRQDAKGWPPGGWYPDQKLSILEALRAFTIDAAYAGFEE
                     TQKGSLTVGKMADIVVLSKDITTADPREILNTHVVWTMVGGKIVYRQP"
     gene            complement(9706..11298)
                     /locus_tag="PK754_04620"
     CDS             complement(9706..11298)
                     /locus_tag="PK754_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HNB08818.1"
                     /translation="MPAHDLLVTAILLLFIAIIVSIGIRRIRLPYSIGLVVAGFAVGY
                     LRVVPSFSITPELVFYIFLPPLLFDAGFNANLTNLKQNWKIIFTLAVPGTIVSVALVG
                     SIAHYAMDVPWMSALLFGALIVPTDTVSILSVFKELKIPTRLSTIVEGESLFNDGTAI
                     IVFKLILGLILAEQYNVTDINYASFSWHLLLSYTGGLLLGAAVGYGAGWLMRRLKDPL
                     VEIMFTVMLVYGMYIVAEKIEISSIIAVVTASMMIGHYRQKLGMSPTTQIALSSFWNF
                     TAFAINSILFILIGLQLNFNNLLENIGIILIGLLAVNIGRIAFIYPFSNILNIFLGRS
                     ASTKHAVIPIKWQHVMALGNLKGSLSMALVISLPESLLYKNQLMTLTFGIVFLSLVIQ
                     GTTLRPILRRFQLQTIPPEQIEFDKRQGLILSAKAVLASLQSEYTSGMITTSVYNTLK
                     EQYEFTIENAEKSLHRLQEKNPSLADAHLQSAYYQMLILQRTVIQNAQTHNIISDDAA
                     ADLLKNFDQQMAALTWSNQDKS"
     gene            complement(11327..11833)
                     /locus_tag="PK754_04625"
     CDS             complement(11327..11833)
                     /locus_tag="PK754_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNB08819.1"
                     /translation="MLEVEFLKKFPLFNGLSDAQLGKLKSIMRETDFETHQIVIRDGD
                     HGEEMFVLLDGDVEISKPMMLKVETEMAKGKDKSLIRLSSKFYACFGEMALFEEKSER
                     SATVTTVTHCQMAIITRHDFTKLVETDYELGYIIFRNMAKIISDRLKKANKDILKLTT
                     AFVLAVQQ"
     gene            complement(11855..13036)
                     /locus_tag="PK754_04630"
     CDS             complement(11855..13036)
                     /locus_tag="PK754_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HNB08820.1"
                     /translation="MQRFWETRFYRRFKRRALEIANNTYFQIFTGILLCVHIGGLLIS
                     FLEDTSANQASTAIRTWYEGIWWAFVTVFTIGYGDYFPITTGGRFLAVGIMVSGVALI
                     SLLTATISSAFVEQRIRKGQGLEKVKLKGHVVICGWNFNVESIIKTLEAELDYPSIVL
                     VNSCDPSPIGEIINAHTNSEISFVAGDFSKDAILDKANIRHAECVIVVADSNELTSNK
                     ADEKTIITVLTVKNMNPKIRVFAHVINPDNAPHLRRAKADDIVISDKYSGFLLAMHVT
                     NPGVPRLFDELLSIEGNEIVRLPIPDTFIGKPFWELTNHYKDKNDCMLIGIAQETRTI
                     AMDDLLSDDNSYLDQFIRAKLQESGKRFAEEERVKIKLNPSKDLVIENNMVAVVLGNK
                     F"
     gene            complement(13131..14981)
                     /locus_tag="PK754_04635"
     CDS             complement(13131..14981)
                     /locus_tag="PK754_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNB08821.1"
                     /translation="MLSSKTLRIIILVFAVTALFCFLPGAAFAGGGEEGGHSLIQSIG
                     ISIIAATVLAFTFHFLKQPLLLSYIAAGVIIGPIGLHLISSKEDIETISEIGLILLLF
                     MIGLEIDMKKLKESGKALIMSGVFQFVLCVALGLAFFPLLGFSIGGGKYDLLYLAACC
                     ALSSTAIVVKLLYAKFELDTLAGRITLGVLVFQDIWAIVILGVQPNLASPDILTILMS
                     FGKGGLLVGIALLISKYILPKLFKSIAKIPELVLVASLGWCFLICSLAGFLGLSIEMG
                     ALIAGVAISTFPYNLDVIAKVISIRDFFVTLFFVALGMKIPNPMDDLGILAIAAVTAV
                     FLIISRFLSVYPILYLLKQGNRISLLPSINLANLSEFSLVIASLGYSAGHIDNKILSI
                     IIFVFVITSIGAPYLIKYSLHIQTALGKFLPKIGLKDVQTVIEKKEEEEEKDIALLGF
                     FRVASSLIKEIQDDKNANNDKAEHEDLFDKIVVVDFNPNVHAKLQALGVKAMYGDISN
                     MDTLHHAGIHGAKLVISTIPDTVLVGTDNLKMIRQIQSLCPHAKIIVTAESAQRAQKM
                     YAEGADYVFLPRVLAADHLKQVIGEALNGDLEKIKKAHIEKLKTVDEIIN"
     gene            complement(15156..15863)
                     /locus_tag="PK754_04640"
     CDS             complement(15156..15863)
                     /locus_tag="PK754_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018619493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNB08822.1"
                     /translation="MNALIVDDERLAREELRRLLSSHPDIKIVGEARHAEEARELIAT
                     LRPDLVFLDIQMPGEDGFALLASLESTPEIIFTTAYDQFALKAFEVNALDYLLKPIDP
                     KRLADALQKISSQESSATPTGPLTDNDQVFVKDGDRCWFVRLKQIRLMESEGNYTRLY
                     FDKEKPLILRSLNQLEVRLDPKVFFRASRQHIINMQWIETIEPWFDGGLNVTLKSGPQ
                     VKISRRQAQKFRELTSL"
     gene            complement(15860..>16138)
                     /locus_tag="PK754_04645"
     CDS             complement(15860..>16138)
                     /locus_tag="PK754_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNB08823.1"
                     /translation="QMLVENAIKYGIADRTQGGTVIIQTERIGSTMEITVINDGHLRQ
                     TDASTGIGNANIRQRLALLYGSSAALELTEKNAQVYAKVKIPIREEKP"
BASE COUNT         4376 a         3460 c         3878 g         4424 t
ORIGIN      
        1 atagttatgc gttggcatga aattggtttg ttgatggttg gtacacagaa gtattgacca
       61 acaacaagga gtaatgtatg tcaaaatcag tagcgctaat agtaaccatc gctttgttgg
      121 cgatcggttg cgacaaagat ggggcgatcg tcaaatccga cgtcggttct gatcaaacgc
      181 ttattatctc gcatgatccg aataaaggcg gcacaatagg agcagtttta cccccaccag
      241 atagtatgtt gtatgtcttt ataggcaata cagatacaag tagtagtagt acttcgaaat
      301 attttatgtt tctcgaacat gacctcaaca ccaatataaa cggacgaaca ctcaaaaagt
      361 atttagatta cggtacgctg aatgtcggcg gttttgcagc acaaagatat catacatact
      421 atgtcgtgaa tgttccggat tcgggtggaa ttccggcgca taaagataca gttgacgcca
      481 cacgatatga tggtttcccg ttgacccttc cgttggatcc tgtgaataat aaactgacaa
      541 tatccggttc taattccaca catattaata atttttctac ggatatttct gtcccaacgc
      601 aactcaaatt tatcaatctt acattcaacc aaaccattgg cgataatgaa gatttacact
      661 taaaacttaa ccgggtttct ccggcaggtt ttgttgggtt tgttccccaa aacattaaat
      721 ttggggattc aacatcggtc ggttacatat attctttctt tgattatact gtacctacgg
      781 atgaagtagt aatttcaaaa caacacttag aacagctaat atccaagtgg aatcaagatg
      841 aagtgatata tcatttattt gtgtttgagg aaaatgtcgt tggttcaatc aatacgattg
      901 ataaaactaa tggccaatca tatggcttgc cggttgagat tgattatacg cataatgttt
      961 tagtcaaatt gaaaaaacaa taacggagaa cctacggcgc tggctacaaa agtcagcgcc
     1021 gtttatttta tgaagaagat cgttgcttgc cttagtttga ttttgttttg caattcgtta
     1081 cttacggcgc aggaacggcc ggtgatccgg attgctatcg gtaaacacga tgtagagcat
     1141 ctgggtggat ggcctttggc tcgacgatgg tatgcgatcg ccgcacaaca tgctaaacaa
     1201 aacggcgcgc aagcgatcta ttttgatatg gcgtttccct cggccgacgc gttgcatccg
     1261 gagtcggacg aagtttttta tcatgagtgc gtaagggatg ataagatttt tcttatgacg
     1321 gaacgtgttc aggaaaataa tactatactt ggcggccaat cgtttcctgc cggtcggaca
     1381 tttcttccgt ttgcggcaca ttggcaaatc gaaaatgcct acctgatcta tacgccgggt
     1441 gatcattcgc tggtttcagc tttaacagaa gtacctgaag aaaccgttat attgcactgg
     1501 ccatccgata ctttacttcc ggatatgcat ctgagtgcgt ttttgtcgtc caattcggat
     1561 ttgagcaacc gaagtgttgt attttatttt gactatcccg gtgtaacctc gtatgtagcg
     1621 tcacctcaag gttcggttaa ccccgtcagt cttattcaga tatgggctgc ggaggaaatc
     1681 aaacaaggtc gtatgtggaa acaatggccg ctatggttgc tggttgttct tttcgccggt
     1741 acggcagtag tgagcttttt gtcgcttcgt gatacacgtt ggatttggat aggcgccccg
     1801 gcattgattt ggtgtgcgtt cgctgccggt ctatactttt tgcgtattta tttgcctatc
     1861 ggtataggtt ggactcttgt gccgatactc gtttcgacgt tcatgttggg gatcaagcgt
     1921 cgcaccgtta ctgtgacaaa agaggttgcc tctgccgcat cggatgatct gacggatcta
     1981 aggtatcggc tcaattttta cgaaaaaata tccgatcaaa ttcgcccgtt ggatttgtcg
     2041 gaatgccgtg aagcgcaggg tatttattac cataaagatt cgcccgtcgc agagcttctc
     2101 caaaaagccg atcaggtagc ccagtcaaat gttccggtgc tgatcaccgg tgaatccggc
     2161 actgggaaag aacgtatcgc cgaatttatt catcgccgtt cacagcgcgc ttcgggtcct
     2221 tttgtagctg ttaattgtgg ggcatttaac gacaatttga tcgaagcgga gttatttgga
     2281 tatgaaaaag gcgcttttac gggcgcggta caagccaagc caggccggtt tgaaatggca
     2341 gataatggta cgttgttttt ggatgaaatc gcagagacaa gcccggcatt tcaggttaaa
     2401 cttcttcggg ttttacagga aggtcggttt gaacgcgtcg gcggcgttgt ttcgaaaagc
     2461 gtatcggttc gtgttattgc ggcgacccat cgggatatat cggtattgat ccgggataaa
     2521 cagtttcgcg aagatttata ttatcgtatt gccggttttg tgttgccgct gaaaccttta
     2581 cgcgaacgta cggttgatat tgattttcta ttccgtcagt ttgtgtacga aattgctccg
     2641 gaactcaaat actccgatca tctcgtccaa tggctctgca cattggcttg gcccggcaat
     2701 gtgcgtgagc taaaagcagc aactcagcgc agtgtgatca atgcacgcat gcggcatcgt
     2761 aattatattt taccggcgga tttcgaaatt cgcggcgcaa cgttttctga ttcagagcaa
     2821 tctttggccg agcgggtgct ccatgcactc cgcgaccatc aatttaaaca tcgtgccatg
     2881 tctgcggtag ccgatgatct gggtttacat cgcatcacag tgaccgaata ttttcgcggc
     2941 tgggtgatca aggcgatgat acggtgcgaa caggaacctc aggctatgtt tgaatattta
     3001 cgcggcacat cggatatatc cgaaccaaag gctttgaagc agcgcataga tgaatatcaa
     3061 aacaatattt ccgaacgcat tcgcacgggc gtgaaaaaaa acgaatctcc ggaagaggtg
     3121 atacgtttac gatttcgcaa tacgcctaaa tttttttacg atgaccttat ccaactcatc
     3181 aaggaatatc gtcatgacta gatggggact attgatgtta gtttttgtat ccgcatcact
     3241 ttttgcgcaa aacgatgcgg atgcgtatcg gcctatgcgt tatgatgcac gcctcacgtt
     3301 tgtatcggag tacaccgctc gccaatttgc ttcaaaagaa tacgtcgcat cgcggattca
     3361 ctatatgcac aatcacggcc tgtatggctt ttcggtattg gtgaatatga atcaacaact
     3421 accctttacg ataatgcccg gcaccacgac gttggtgttg cccgacagcg ttaaattagt
     3481 ggatcgtagc caaatcacta accatgcgca cacgtatgtg gttgatttac cggcggctct
     3541 tgagttgcgc cgccgttttg cacgatttga aattgctttc ggttcgcaaa ttctcgttga
     3601 aaaagaagaa atcgtgtcaa ctttggtaaa agaactgaca tcgaaagtgt attacgtgga
     3661 ttatgaattt gcatcccatc gccgttcgtc actattaggc tatggtgcga tgacaagcta
     3721 ttggggtccg tttgccgtaa cgacaggcat attgccttgg cgaatgatac gcatgggtaa
     3781 cgacagcatg cattatgcat tgaaaaataa gttgagcggg tttttagatg ttcaatatcg
     3841 ttaccgttcg cattcgatga ccacgcgtgt aggccgggat gcttggggcg gtaatgtaca
     3901 attgaaatac gatgcgttac aggtgaacaa tgaatattgc agaagtttgc ggccgtcgga
     3961 ttatcaagtg tggaaattaa atctaagtta tgatttgagt ggcggcttca cattacttgc
     4021 cggttacgaa agcacatggt atttggcgga aaatttcaga tcccaaaatt ttcgtcaatg
     4081 gcttaatcgt cacggcgaca tatcgagcgg aaaacttacc aatcagttgc ctcacaaaac
     4141 ggtgcaaatc ggattgcgtt atgatttgtc aggaagccgc gctgccgatt acgtaaaagt
     4201 ggtgaatatg aaaatttttc agacaaaagt ttatacggcc aaacgcgcct attatgcata
     4261 caatccggtg ggacagatgg atgtacataa tgcgtcggat gaacacctga acgttgtcat
     4321 acatgccgaa gccggtgcgt ggggcgaaat atttcgttca tcttcgattg atttagcgcc
     4381 acatgagatc aaatcggtgc cgttattttt ttacataccg gatcagagcg tagaacaatc
     4441 cggcatggtg gctcaggtga aaattaccgg tgaagtacgc ggcaaacaca tcgcactgtc
     4501 cagttttccg gtgacattat atgatatcca tagctgggat ggcgatacgt ggggattgcg
     4561 ctactttata gctccggatg atcctgaaat acaatcgttt gccaaaagca atttgttgac
     4621 cataggaacc gattccgcgt attcagaatt gtataataag tttcatacgt taaaaaaatt
     4681 tgtaacggct gtcgggaaaa acttgcgtta cgtatccgac cctaccacga cttttgtcaa
     4741 tgaacgtgtg caatatcctt cggagacact gcgacaaaag tccggcgatt gcgaagacct
     4801 tgttgcattt atggctacca atcttatggc tataggccat gcttgcgccg tggtggatat
     4861 tcgcccgacg ctcactgaaa atatcaatat tccaactgcc aaaccgggtt ccatagggca
     4921 tgtatttttg cttgtggata cgagtattcc ggttaatgcc gtggatcagt taggtttgtc
     4981 cgaatttgaa tatgttgccc gccccgataa aaccggacgc gtgacgattt ggttgccgct
     5041 ggagacaacc gttgtaaacg gcggttttga acatgcgcat cgcgaaggtg tgcgccagta
     5101 ttatgatgaa gtgattcaaa aaaacggagt agtccatgga acggtacacg tatatgattt
     5161 ttaaaacgat atgctatatt cttcttacct caccattgat ggggcaatcg gaaatttggc
     5221 taaaactcga aaagggcgat gcgatgtatc tggatgttac atccatgaag tggcagcctc
     5281 tagggagtaa agaaaaaata gcgccgcgga cgtatgtttt gactaaagcc gatgccacgc
     5341 tcaaaatcta tcgcgaaacg aatgtatatg atgcgccggc cggagggtat ttttacatcg
     5401 aagatatgtt cgttaataac aaacgcgact tagtttctga gatcacacgc atcgaagcgg
     5461 agcagcttcc ctcgcaaccg gttgaacgag ccaacgatca aaaatttggt cttacctatg
     5521 gccgggctaa cgaaacgctc gctgaaaatc aatccgtacc gtatttgtcc gagcgtatgc
     5581 gcgcaatccg ttggtttgaa gagcatcagt atgcggaaac tgcgttatta agtctcaaac
     5641 gactaatttc caaatttcct tcgttatatt ttgaaccaag taattttgat cgcttgtgtt
     5701 cattgtatat gaagttgggt ttatatggat ttctttttga tgagaccaat cgttttatga
     5761 cgttaaaaag agatgatgaa tttggtcaaa tggttattcg ctggaatgaa acagccaaga
     5821 agaaactaac gacgtcaggg caatgaatcg aattcatttt ttagaatgtt tttttagaat
     5881 ataataagtg tctgtgattc tcgtcggagt cggttttctt aaattcattt tgaaacaata
     5941 gtatccgcac gtcgtaccat ttacgtaata aattacatta atttaaccta ggtgcgacat
     6001 gcgtgtattt atttttattg tggccttctt tttaacaagc ctcctccatg ctcgaaatgt
     6061 tttttcgcag gttatggcta tggattctac gatatgggat atgcaaggcc aaagcgggtt
     6121 tgtagattat caaggtcgga agagtatttt tctcaatggc ggcgccgccg tcttaaaaaa
     6181 tttggaattg cgtgacggtg ttatggacgt tgacgtttcg acacctgcgg cgcgtggttt
     6241 ttttgggata caatttcgca tatccgaaga cggccttaac ggggaatggg tatatctccg
     6301 ccaacacaaa tccggacttg ctgatgccat gcaatatacc ccgatcctca atacaggact
     6361 caattggcaa ctgtacagcg ggccgggatt tatcggcgct gtggatatac ctaaaaacga
     6421 gtggttccat ttacggctcg aagtttccgg agcgcaagcg cggttgtttg taaaaaatat
     6481 ggataagccg gtattggtta tggacgatct aaaaagcgga attcagaagg gctctatcgc
     6541 tttagcggtt ttaaccggcg cgacatattt ttccaatttc gaagttaaaa aaactacgga
     6601 tgctccgtgg gtgagaaaac atccggctat gctgcctaat gtattgaagc aatgggaaat
     6661 ttcaccgtcg atggatgcgc tggcacgtaa cgtcgaatca cctctgactc aagctgagaa
     6721 aaaatcaata gtatggcaat cggtagaaac ggaggcgccg ggcatggtga cactttctcg
     6781 ttatcgggaa gcaccccatc tgcgggtttc ttttgccaat gatttttcta aacgattgga
     6841 agcgcaaccc ggttctaaag tagtttatgc taaaacaaaa atcacgtcgg atcgcgatca
     6901 aatcaaaaaa ctgtatatcg gatacagcga tgaagtgtgc gtattcctaa acgggcaaat
     6961 actctatcgc gggcgcagtg cacagtattt tcgcgatcct ggttttttgg gaatcatgga
     7021 ttccgaaaat gatgcgcttt atataccttt gaaaaaaggg gataacgaat tgatcctggc
     7081 tgtcagtgaa ttaggcggcg gatggggatt tatttgccga ttggaataat acgagagcgg
     7141 gattaattct gattggtaat cgaaatggtc catgtcgttt ctagcatgtc ttccgggaaa
     7201 tcattcggta atggatcaag cggaaaacta tttacgacag acgcatacgc gggcgcaaca
     7261 aaggatttat cgcaattata atccacgacc ttggagtcaa ccaattggcc ttgcctgttc
     7321 attttgacgt agatgagtat ttcgccgaca cgtgcaaact gactgaaaaa tattttggat
     7381 gtgatgcgat accaattaga agtcatttta tttttccatg cgagcatata cggagcaaat
     7441 tcccacttat acgtattgag ttcgtaattt tgtccggcaa atccttgttg cgaaggatct
     7501 tcctgaccgg ttaatcgctc ataaacggac ttctgattgg tattgcgctt caaaaaaggc
     7561 tgtacgttca tattattctg aaaacgatct tcatcggtgg attcatccgg cggagttgta
     7621 ttttctgttt tagggcgcgg ttcttggttt tgtgtcacat caaccgaccc gaaatttttg
     7681 atttcgtccg gtggtttgac gtctgtggta gaattgcgat cggataaagc atccggtttt
     7741 tgcgcgacct tatcgttggc tttcggttgc tcaagcaaat cacgcacctt actcatatcc
     7801 ggttcttcaa gtaattttag taaaacggct ttttcgctat cgctgataag ttgcggtggt
     7861 ttggtaggat tgactacgat ttcgatgaaa aaaattgccg caagatttat caaaacggat
     7921 aacacgacgc cgatcatcaa aaaaatctgc tgcgtcgtca ggcgcggtat gtaccaaaga
     7981 aggagaaaac ctccgatccg aatgcgaatt ttcatggttg acgatatacg atcttgccgc
     8041 cgaccatcgt ccaaacgaca tgggtgttga gaatttcacg cggatccgca gttgtaatgt
     8101 ctttggataa aacgacaatg tccgccattt ttcccacagt gaggcttcct ttttgagttt
     8161 cttcaaaacc cgcgtaagcg gcgtctatcg taaaagcgcg taaggcttca agtatggata
     8221 atttttgatc gggataccag ccgcccggtg gccatccttt ggcatcctga cgcgtaactg
     8281 ccgcatagac gccgagcaat ggattatgcg attcgaccgg tgcatcggaa ccggcggcca
     8341 aacgcacacc gtttttgata aatttctgcc aggcataggc accccgaata cgatccttgc
     8401 cgacacgctc ttccgcccaa tacatatccg atgtacagtg tgtgggttgc atcgaagcaa
     8461 tcacgccgag ggttgcaaaa cgcgggatgt ccgcttcatt aacgacttgg gcatgttcga
     8521 tacgaaagcg tttatatgtt gcgctgtcgg gcaccgcttt catggcggct tcgtaagcat
     8581 cgagcgtatt gcggttgccg cgatcaccga tcgcatgggt acaaacctga aagccccact
     8641 ttagcgcatc ggtggcgatg atttcaaggc ggggtttcat catcaattca aggcctttgg
     8701 attgcggact gtcagaatac ggttccagca acgccgcgcc gcgtgaaccg agtgcaccgt
     8761 cggaatacag cttgatagac gcaattgtaa ggaattggtt gaaaagattt ttctcggggc
     8821 cttttttgta atacatatcc aaaagccggc gatcaaaacc attgagcatg gcataaaccc
     8881 gcacgcaaag tttattgcga cgtcccaatt ctttatacac gtcaatatga ttggtatcaa
     8941 cgccggcgtc atgtacggat gtgagaccga ctttgaggca cgcgctcatc gcggcttcca
     9001 acgcgagact gtcttcggaa agtgtatatt tgggaatata gggtatcagc aagtctatcg
     9061 cattatctat gagtacgccg gtgggttcgc cctgcgcatt gcgcattatc ttaccgcccc
     9121 gcgggtcggg cgtttcacgg ttaattttgg cggctgtcaa aacattttga ttaaccaata
     9181 ccgcatgccc gtcaacgcgc tcaagaacga tgaagtgatc cggcgaaaca gcggtcagtg
     9241 cggcatgttc gggaaaatct tttttagacc aatcgttttg atcccaaccc cggccgcgaa
     9301 tccatgcgcc tttgtttgta ttcgccacac ggtcgcgaac cagcgtaatg acctgatcgt
     9361 acgattccgt gcctacaaga tcaagttgcg taaggtaatg accgatattg agcatatgca
     9421 gatgcgcgtc gattaatccg ggaagcacaa gttgaccttt gacgtcttcg atacgattgg
     9481 tcacatattt ttttttcatc acttcggcct tgcccatatc aacgatccgg ccatcgcgaa
     9541 tggcgatgga ttcgatttcg ttattattta gttcgtcgtc catcgtgaca aatttcccgt
     9601 tgacgacaag catgtcaata ttggcgcgtg tgccgcatgc gctgaggcac atcaaaatga
     9661 tgatcagtat ataacggtgc atgggtggtt ctttcgtata taaaattaac ttttatcctg
     9721 attgctccac gtgagcgcgg ccatctgttg gtcaaaattt ttcaacaaat ccgccgccgc
     9781 atcatcgctg atgatattgt gcgtctgtgc gttttgtatg acggtgcgct gaaggatcaa
     9841 catctgataa taagcagact gcaagtgagc atccgctaaa gacggatttt tttcctgcaa
     9901 acgatggagg cttttttccg cattttcgat ggtaaattcg tattgctctt ttaatgtgtt
     9961 ataaaccgaa gtggtaatca tgccgctcgt atattccgat tgtaatgagg ccaaaacggc
    10021 cttcgccgaa agaatcaagc cttgccgttt atcaaattcg atctgctcgg gtggtattgt
    10081 ttgcaattga aatctacgta aaatcggacg caaagttgtg ccttggataa cgagcgaaag
    10141 aaaaacgatc ccaaatgtca gggtcattag ttggttttta tacaacagcg attccggcaa
    10201 actaatgacg agtgccatcg agagcgatcc tttgaggtta ccgagcgcca tgacgtgttg
    10261 ccatttgatc ggtataacgg cgtgtttggt tgaggcactt cgaccaagaa aaatattgag
    10321 aatgtttgaa aagggataaa tgaaagcgat acgtccgata tttacagcca aaagcccgat
    10381 cagaatgatg ccgatatttt cgagcagatt attaaagtta agctgtagcc cgatcagaat
    10441 aaataatatc gaattgattg caaatgcagt aaaattccaa aacgatgaaa gtgcgatctg
    10501 tgtcgtcggt gacataccga gtttttgccg gtaatggccg atcatcatag aagccgtcac
    10561 caccgcgata atgctggaga tttcgatttt ttcggcaacg atgtacatac cgtaaaccag
    10621 cattaccgtg aacatgattt cgacaagcgg atctttgaga cggcgcataa gccaaccggc
    10681 gccgtaacct acagcggcac ccagcaaaag cccgcccgta taagataaaa gcaagtgcca
    10741 cgaaaacgaa gcataattga tatctgtgac attgtactgc tcggcaagga taagcccgag
    10801 aatgagtttg aatacgatga tggccgttcc gtcattgaac aaactctcgc cttcgacgat
    10861 ggtggagaga cgtgtgggaa ttttgagttc tttaaaaacg gatagaatcg aaacggtatc
    10921 tgtcggaacg atcagagcgc caaaaagtaa ggccgacatc cagggaacat ccattgcata
    10981 atgtgcgatc gaaccgacca acgcgaccga cacgatcgtg ccgggtacgg ccagcgtaaa
    11041 tatgattttc cagttctgct taagattggt caggtttgca ttaaaaccgg catcaaaaag
    11101 aaggggcgga aggaaaatgt aaaaaaccaa ttcgggggtg atggaaaacg aaggaacaac
    11161 gcggagataa ccgacggcaa aacccgcaac gactaagccg atgctgtagg gcaaacggat
    11221 acggcgaata ccgatcgaaa cgataatcgc gatgaatagt aacaggatcg cggtgaccaa
    11281 aaggtcatga gcaggcatcg ggactccgtc ggaaaaataa taccattcat tgttgtacgg
    11341 ccaaaacgaa cgccgtggtt agtttcagga tatccttgtt cgctttcttc aaacggtcgc
    11401 tgataatctt ggccatattc ctaaaaataa tatagccgag ttcgtaatcc gtttcaacga
    11461 gtttcgtgaa atcatgccgc gtgatgatag ccatttggca atgggtcacg gtggttaccg
    11521 tagcggagcg ttcgctcttt tcttcgaaca aagccatttc gccgaagcag gcataaaact
    11581 tggaactgag acgaatcagc gatttatctt tgcccttggc catctcggtt tccactttaa
    11641 gcatcatggg tttggaaatt tcgacatcac cgtccaaaag tacaaacatt tcttcaccgt
    11701 gatccccgtc gcgaattaca atttgatgag tctcgaaatc cgtttctctc atgatggatt
    11761 taagttttcc cagctgggca tcgctgagac cgttgaaaag cggaaatttt tttaagaatt
    11821 ctacttcaag cataaatcat ccggtttttg acgattaaaa tttattaccc aataccacgg
    11881 ccaccatatt gttttcgatc acaaggtctt tcgagggatt gagttttatt ttgacgcgct
    11941 cttcttcggc aaaacgtttg ccggactcct gaagtttggc gcgaataaat tgatccaaat
    12001 acgaattatc atccgataag agatcgtcca tagcgatcgt tcgtgtttcc tgagcgatcc
    12061 cgatcaacat gcaatcgttt ttatctttgt agtggttggt cagttcccaa aagggtttac
    12121 cgataaatgt atccgggata ggaagtcgca cgatctcgtt gccttcgatg gaaagaagtt
    12181 catcgaaaag ccgtggcaca ccgggattcg taacatgcat ggcgagtaaa aagccgctgt
    12241 atttgtcgga gatgacgata tcatccgctt tggcgcgccg gagatgcggg gcgttgtcgg
    12301 gattgatcac atgggcaaaa acgcgaattt tggggttcat gtttttgacc gtaagaacgg
    12361 tgatgatcgt tttttcgtcg gccttattgc tggtgagttc gttggagtcg gctacgacga
    12421 tcacgcattc ggcgtgccgg atgttggcct tgtccaatat ggcgtccttc gaaaaatcgc
    12481 cggcgacgaa actgatttcc gaattcgtat gcgcattgat gatttcaccg atcggtgaag
    12541 gatcgcagga attgaccaat acgatcgaag gatagtcgag ttcggcttca agtgttttga
    12601 tgatggactc gacgttaaaa ttccagccac aaatcacgac gtggcctttg agttttactt
    12661 tttccaaacc ttggcccttt cggatacgtt gttcgacaaa cgcggaggaa atcgtagcag
    12721 ttaagagtga aatcagtgca acgccgctta ccataattcc gacggccaaa aaacgtccgc
    12781 cggtcgttat cggaaaataa tctccgtaac cgatggtaaa aacggtaaca aacgcccacc
    12841 aaataccttc ataccaggtt cggatagccg tcgatgcctg attcgccgaa gtatcttcta
    12901 aaaaactgat cagcagtccg ccgatatgta cgcagagcaa aatgccggta aaaatctgaa
    12961 agtaggtatt gttggctatt tcaagagcgc gccgcttgaa acggcggtag aaacgcgttt
    13021 cccaaaatcg ttgcatacgt cgtacgaaaa cctctctgat acgaggatca ataggattta
    13081 tagtgatgaa gcgggaatgc tcctgattca ttcccgcgat caatataaac ctaatttatg
    13141 atttcgtcca ccgtttttaa tttttcgata tgggcttttt taattttctc cagatcgccg
    13201 ttgagggctt cgccgatgac ttgtttgagg tggtcggcgg ccaaaacgcg gggtaaaaat
    13261 acataatccg caccttcggc atacatcttc tgtgcgcgtt gtgcgctttc agcggtcacg
    13321 atgattttgg cgtgagggca caaactttgt atctgacgga tcattttaag attgtccgtt
    13381 ccgactagga ccgtatcggg aatagtggaa ataaccaatt tggcgccgtg aatacccgcg
    13441 tgatgcagcg tatccatatt ggaaatatcg ccgtacatag ctttgacgcc gagcgcttgg
    13501 agtttcgcat ggacattggg attaaaatcc accacgacga ttttatcgaa caggtcttcg
    13561 tgttcggctt tatcattatt ggcgtttttg tcgtcctgta tttctttgat caatgagctg
    13621 gctacacgga aaaagcccag caaagcgata tccttttctt cttcctcttc ttttttctcg
    13681 atcacggtct gaacgtcttt gagtccgatc ttggggagaa acttacccaa cgccgtctga
    13741 atgtgaaggc tgtacttgat cagatacggc gccccgatgg atgtgatcac aaaaacaaaa
    13801 atgatgatgg ataaaatttt attgtcaata tgacctgcgc tgtatccgag cgaagcgatg
    13861 acgagcgaaa attccgacaa attggccaaa ttgatggacg gtaaaaggct gatacgatta
    13921 ccttgcttta acaagtacaa tatcggataa acggataaga agcgtgagat gataagaaat
    13981 accgccgtta cggccgcaat cgcaagaata ccgagatcat ccatcggatt cggtattttc
    14041 ataccgagcg cgacgaaaaa taatgtcacg aagaaatcgc ggatactgat gactttggca
    14101 atcacgtcca aattgtacgg gaacgtcgaa atagcgacgc cggcaatgag ggcacccatt
    14161 tcgatcgaga ggccgagaaa tccggccaat gagcaaatca aaaaacacca tccgagcgaa
    14221 gccacgagta caagctccgg aattttagcg atggatttga aaagtttcgg aaggatgtat
    14281 ttgctgatca aaagagcaat acctacgagc aagccgcctt tgccaaaaga catcaggatg
    14341 gttaaaatgt ccgggctggc gagattgggc tgtacaccca gaattacgat cgcccaaata
    14401 tcctgaaata cgagtacacc aagcgtaatg cgtccggcga gtgtgtccag ttcaaactta
    14461 gcatataaaa gctttactac aatcgccgta ctggacagcg cgcagcatgc ggcgagataa
    14521 agcaggtcgt acttaccacc gccgatacta aatcctaaca aagggaaaaa agccaggccg
    14581 agcgcaacgc aaagcacaaa ttgaaatacg cccgacatga tgagagcttt tccggattct
    14641 ttgagctttt tcatgtcaat ttcgaggccg atcatgaaga gcaaaagtat caatccaatt
    14701 tctgaaatgg tctcgatatc ttctttactg ctgatcaaat gcaatccgat aggaccgatg
    14761 atcacaccgg cggcaatata tgacaaaagc aacggttgct tcaaaaaatg gaaagtaaaa
    14821 gctaaaaccg tcgccgcaat aatgcttata ccgatactct gtataaggct gtgaccgcct
    14881 tcttcaccgc cgcctgcaaa agcggcaccg gggagaaaac agaaaagagc cgtaacggca
    14941 aacacaagga taatgatgcg taaagttttg gaactcagca taattgggta ctcgcattaa
    15001 tatgaaaaaa tgaatttata gtcgaaccaa acggttagca aaaaaacata aagtaaaatg
    15061 ctatttcatg caaataaaat cggagggaaa atgaggataa ttaagaatga tgagttaaga
    15121 atgatgaatt ctaaattcta aattcactcg tttcttcaaa ggcttgtcag ttcgcggaat
    15181 ttttgagctt ggcggcggga tattttgacc tgtgggccgg attttaaagt tacgttgaga
    15241 ccgccgtcaa accacggttc gatcgtttcg atccactgca tgttgatgat atgctgacgg
    15301 cttgcacgga aaaatacttt gggatcgagg cgcacttcaa gttggttgag ggaacgcaaa
    15361 atcaaaggtt tctctttgtc gaaatataaa cgcgtatagt tgccttcgga ttccatcagt
    15421 cgaatttgtt tgagacgtac aaaccagcaa cggtcgccgt ctttgacgaa aacctgatcg
    15481 ttgtccgtga gcggtccggt tggtgtagcg gagctctcct gcgacgaaat tttttgcaat
    15541 gcatcggcga ggcgtttagg atcaatcggt ttgagcaggt agtcgagcgc attcacttca
    15601 aatgctttga gggcaaattg atcataagcg gtggtaaaaa taatctccgg tgtggactcc
    15661 agcgaagcga gaagggcaaa gccgtcttca ccgggcattt ggatatctaa aaaaaccaaa
    15721 tcaggtcgaa gtgttgcaat caattcacgc gcttcttcgg catgacgtgc ctcgcctacg
    15781 attttaatat ccggatgcga tgataataaa cgacgcaatt cctcacgggc caacctttca
    15841 tcatctacga tcagagcatt catggctttt cctcgcgtat cgggattttg actttggcat
    15901 aaacctgagc atttttttcg gtaagctcca gtgcggcact ggatccgtac agtaaggcga
    15961 gccgttgacg gatattagca ttaccgatac ccgtggaagc gtctgtttgt ctgagatgcc
    16021 cgtcattgat caccgtgatc tccatcgtag agccgatgcg ttcggtttga atgatgacgg
    16081 taccgccctg cgtgcggtcg gcgatgccat atttgatggc attctctacc agcatttg
//
LOCUS       DAOQNQ010000043        16061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1288_length_16061_cov_6.023679, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000043 DAOQNQ010000000
VERSION     DAOQNQ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16061
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1288_length_16061_cov_6.023679"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2355)
                     /locus_tag="PK754_04650"
     CDS             complement(<1..2355)
                     /locus_tag="PK754_04650"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB08824.1"
                     /translation="MLLWMNVTFLAAQTERLYVERISVEQGLSQTTIFGMMQDRRGFM
                     WFGTEDGLNRYDGYQFRIFKNDPFDSTSLSNNYINTLYEDRRRFIWVMTLEGVNRYDP
                     RDEKFLRFKRSDDTTTIAGYRIENMAEDSVGHLWCTTDRGMLLRVDENGKIQERWNPR
                     SADDMSSVQNLQIENGNRFFLSTNSGLYICDPTRRHVVRYHREAAGDYRLSENSVRSV
                     VKDRRGWYYVATYGGGLYMYDEVWRLQNNFKKADASKHGLTEDDLQTLYCDRAGRIWV
                     GSEGGGLFLFSPEQNKFLQFKTESGDAHSLSNNLILRLTEDRTGVIWIGHRDGGISKY
                     DPQRIRFVSYRHDPDHTNSLSHNSVYSIFEDPADSGKVLWIGTNGGGLNRWDRITQTF
                     RSYVNTPGVRHTIGDNSVRFLFKDSEGVFWVGTNKSGLQRMDTDRGLFEPSTNPNIKF
                     IRYVIEDRKDKNIFWVGTNNNGVFRIEKSGKILQQYSPQTKPGLSFNIIRGLYQDSDG
                     KVWVATQGGGLNCISADGQSVTVYNYDPTNPQSLSNSIVLCILPDTTQNIFWLGTAGG
                     LNRFDSRTGITQHFRERDGLANDVVYGVLRDEKGFLWMSTNGGLSKFDPKTSVFRNYD
                     TKDGLQSNEFNAGAFFKNQNGEMFFGGIQGFNVFHPDRITDNTMPVSVVLTQFQIANQ
                     PVAVDPNGVLKQSVTESQQIVLPYAGNAFAFEFSALMYHAPEQHQYAYRLEGFDKTWL
                     YSGTRRYVSYTNLDPGEYIFRVKASNHDGVWNEDGASMMIIITPP"
     gene            complement(2436..3320)
                     /locus_tag="PK754_04655"
     CDS             complement(2436..3320)
                     /locus_tag="PK754_04655"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNB08825.1"
                     /translation="MTAWTERLRDAKPLLLDGATGSELERRGVPCVLPLWSAVALLQN
                     EYTSVLRAIHAEYIQRGAQIITANTFRLNRQTLMTEQLHNDSRRLICKAVDTVREAIE
                     SVARDRTVFIAGSIGPVEDCYHPERAPADDVLRDDHHRRIDDLYAARPDLILIETMNN
                     MNEASIATEYAVQTGLPVAVSFIARNDTQLLSGESLEKAIRRIKTYNPAALMLNCMHV
                     EIMEHALDVLKDTAGDIPIGAYVNIFPEQSKPAMTPEDFASRMEAWVSEYKLKIIGGC
                     CGTTPRHIEKLAERIGVN"
     gene            complement(3369..6065)
                     /locus_tag="PK754_04660"
     CDS             complement(3369..6065)
                     /locus_tag="PK754_04660"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNB08826.1"
                     /translation="MSEAQQKTEIPKAYDPKASEDKWYAYWLKHDFFKAHADSAKPVF
                     TIVIPPPNVTGALHIGHAYNNSVQDIYTRFKRMQGFETLWTPGTDHAGIATQTVVEKQ
                     LAKEGTSRKTLGREAFVNEVWKHKETYRARIVGQLQKMGCSCDWEREWFTMDEKLSEA
                     VKEVFIHFYNKGWIYKGQYIVNWCPYSQSAISDEEVEYKEINGKMYYFRYPVKDSAEH
                     IVIATTRPETMLGDTAVVVNPDDARYKHLIGKKVILPLVGREIPIIADDYVDKEFGTG
                     AMKVTPSHDPNDFVLGKRHQLQFLNIMHADATMNQNVPEKYRGMERFAARKAVVADLE
                     SAGLVEKIQDHLNRVGYSQRGNVPIEPLVSEQWFLKMDELAKPALEAVLKGDIRFHPE
                     KWIKTYEHWMTSIRDWCISRQLWWGHRIPAFYAPDGSVAVARNADEALAILKKKNAQL
                     TVSDLRQDEDVLDTWFSSWLVPFSSMGWPHQTKDLEKFYPTSLLVTGPDIIFFWVARM
                     IMAGLEFTGKVPFCQVYFNGIVRDELGRKMSKSLGNGIDPIAVIDKYSADALRFTLVD
                     LSAEGQDINLAERQFELGRNFSNKVWNAFRFLANYQEQLPSAKPQLPPSNAITELADR
                     WILSRYHRAVEGITTGLENYKLHEAMDSVYRFFWADYCDWYLELIKDRMYNATPEIKE
                     KTLSTALYILDGVLRLMHPIVPFVTEEIWQRLMPRTDGESIMIQPWPQCEASYISVEA
                     EKDLEQLRDTITTIRNIRSEMNIAPSKKTDLVLRCVTPQDAALYNANTSALAGLARVE
                     KITAAVGADKPRIAASAVVNGTEVYLPLEGVIDLEVERTRLQKEIDRISGQIGGIEQK
                     LGNEGFVAKAPPQVLEKERAKLDNFRATVEKLRSNLSALQNL"
     gene            complement(6375..6950)
                     /locus_tag="PK754_04665"
     CDS             complement(6375..6950)
                     /locus_tag="PK754_04665"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012898785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNB08827.1"
                     /translation="MPKPLPRSFYARPTLEVAPELLGKILHHGTMSARIVEVEAYRAG
                     DDPASHAYRGLTPRNKIMFGVPGHLYVYFIYGMYYCANVVCEKEGTAGACLIRAVEPL
                     DGVERMQANRVMARGFALSNGPGKLCLALGIDKSHNGTDLTTDDLYLSDDRYHAKIKT
                     SPRIGIRVGTEKKWRYFIDGNAWVSPSRNNK"
     gene            complement(6943..8754)
                     /locus_tag="PK754_04670"
     CDS             complement(6943..8754)
                     /locus_tag="PK754_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08828.1"
                     /translation="MSENTKITNTQDITDPERLIETDDKFSKSDHVHYKEILHRYYRQ
                     SLPFDIQSYKNLDEQPPDARIAMKNDPFFRDVGKYVHILDFSEQERIKFGRENEVQHL
                     IVFLLFERDLDVLEAVFGNPQLPTKVLYDFINLIKERDIDREDDKILKLAQKVMKRRS
                     RRIVKVREIHTANTQPIQRANLITLFSYLIDDDAQVRMAAENVVSALDVQTLINALED
                     DSLIHTMRESISKITAISIFRMLESAVHLEIRNLAAGGDLGADQTNEHNAARRQLTQV
                     LQDRKRRELEKCVEDPTDLFNATLITYLHFDKDADLRAQALEILQPDDIIDLVSDDST
                     PRHISSQVLPMLEKSTDERVRQRTTEIRLKEAERLNKKMKEIEVSVNAYFDVIFQSLN
                     YSRINSQKEAVTMLRGSLNYLQLFAKEAKNLESGSATVAQGVIRQAIEHFEGSINALY
                     SDTKRELFTEFEEIQGMVRHILDLKSFKFEEESGVEDEVDEAVLNKAVMIWRNTISQF
                     LGRIKDLEEMLRIKWGKLMTENEPKRKQEENESDLNEAFGEIETMHKNEVDCKLKIPC
                     RECKRRGCASERFMLQVDFLLEEIHQGFKHDKQTHHA"
     gene            complement(8747..9577)
                     /locus_tag="PK754_04675"
     CDS             complement(8747..9577)
                     /locus_tag="PK754_04675"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB08829.1"
                     /translation="MLTEANNAVRVCVLGSGSKGNSTFLQYGATKVLIDAGLSGEETM
                     DRLTAVATPINELQHIFITHEHDDHIKGLKKILKSFSGMVHCNAGTFRHLRHRIPEQT
                     RVHVFSDTFEADGLQVQPFSISHDATEPVGYSFQCGHRQVTVATDMGYASELVKARIQ
                     DSDILILESNHDEGMLMKGRYPWPLKQRIASRVGHLSNRQAGELMRDTMFPRLQWLVL
                     AHLSEHNNMPDLAMASMTEHLQTTAMKEQIQVHVAHQHTISAVMELNHSNVTETAHHV
                     "
     gene            complement(9654..10238)
                     /gene="pyrE"
                     /locus_tag="PK754_04680"
     CDS             complement(9654..10238)
                     /gene="pyrE"
                     /locus_tag="PK754_04680"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNB08830.1"
                     /translation="MMKQEEVLGIFKQSGALLEGHFILTSGAHSPNYFQCARVLQHPN
                     WAQLLCSEIALHYKDKKIHAVAAPAVGGIIVAHEVARTLNARCIFSERENGTMRFRRG
                     FEIKRDENVLVVEDVVTTGGSLKEVIQLCRDADANVVGVGYLVDRSGGRAEFGVPKFS
                     LVSVDVVKYEPETCPLCREGALPAIKPGSRSMNK"
     gene            complement(10235..12880)
                     /locus_tag="PK754_04685"
     CDS             complement(10235..12880)
                     /locus_tag="PK754_04685"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNB08831.1"
                     /translation="MIFRIITLLCCALFVQAQNDFRVLGEKNGYDPQTLREYVNLTET
                     RAFLDLAGEWTMSVEGVSRKVIVPSSYDYNGKVTFTRTFVPGEDFRNKHFRFVSLGIN
                     FRADIRINGEFVTNANNSYTRLEADLREDLIRIGQENKIEITVDSRVDPFTSYPRETS
                     LLQPKLYGGITRELFLVGLPKVAIDQYTVGYTLSNDLRKCDFVVNWHLRNYEYSLRLV
                     DTLRLINPNQPLRYILEMFEENNPEPVYTNRFKKYRNVWDIPKIREQNEEDTVSVFNF
                     VDRQLRFSIEKPVFWNPITPSRYRVVMYLVQGTTVIDRVEYQLGIAQADVHDQYIFLN
                     AQPVTIRAVEYYEDYPGAGSTMSWAMMEQDILKIKRLGANAVSFRHHPPHPYFVELCN
                     RYGLLLFYEIPSWRLTADIMRTPSYVEDIRKYYRHIVEYEHLNPSILAWGVGFAPDPS
                     DGAANYYFQQIVGEIKNKDRRPVFAASAFGGYDSFMDNVDIVQLDIRLNSPLSSNLFV
                     QRSLAKWPTRALIAVYGAQIFSNNQNGYSDPMSTKFQAKVLADFYKKTGEWKLAGGVV
                     RSFNDFAVQRPYLYANPNASSTIYTSGVVTYDRQERYAFETLRALYSDDALEPIAVGA
                     YEPVYPKTYPITGLILVVLFISFYRQSPKFKNSVHRSLFKVVSFFGDIRENRVITLWP
                     ALLVGFLASLAYAALLSIVFFELRRNHLFDEYLATFLRSNGLKAWVDGLAWSPELFVL
                     EWTMIYALVITVIGLLFMIFQKPFQAYVNFQQSIIAAAWSSSHFLYLIPFVVVFHRLM
                     KTDFVLSLGATAAVLFFFWHWIRFFRAAQILFNASWWRTGFILGGALIVLCTALGIYY
                     SGSHDFFNVWNYIEQVSSSKNYTTE"
     gene            complement(12919..13827)
                     /gene="rocF"
                     /locus_tag="PK754_04690"
     CDS             complement(12919..13827)
                     /gene="rocF"
                     /locus_tag="PK754_04690"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014463312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HNB08832.1"
                     /translation="MSVIRLIGVPLDLGAGRRGTDMGPSALRLAGLTERLQELGHTVK
                     DKGDIVTKNPEVIRVGDEKLKYLNEVTRAVTALADDVEKCLADNHIPLVLGGDHSIAI
                     GTLAGVSGYFRKNNQKIGVIWIDAHGDMNTPDTSPSGNIHGMPLAVSLGHGHPKLTGV
                     GGEFVKVNPENTVLVGIRTLDDGEIDNIRRMGVTVFTMRDIDEQGMSKIIRQAIDIAA
                     RDTAGIHVSFDADSMDPSIAPGVGTPVPGGLTYRETHLAMESLCDSKKVISAEIVETN
                     PILDIKNTTAHVGVEMMASLFGKRIM"
     gene            complement(13883..15604)
                     /locus_tag="PK754_04695"
     CDS             complement(13883..15604)
                     /locus_tag="PK754_04695"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HNB08833.1"
                     /translation="MANDQIRYERILMLALHSQKYIVATGLAVLVAAFLMPCPGWAQY
                     TGPSVNLPMGHFGYDLIEHLETRGVVSPSIRLRTKPYDRGTVLKIINEVQDKIKTDAN
                     VLTSAERSLFEKLKGEFYVELTSTQNIQTSDVREKHIFEYQTHEDGFSYLVGDLVIDQ
                     SVELTRFSKKNDSLGGTIGLSKGSGRVRGLIKGGIAFYADASNTLVKGDEQSTDFGSS
                     YRGGVLNYNPASANRYALHSDAYIVIEPKWFRLQVGKDNMAWGPGYYDNLLLSGNAPS
                     FENLRLDVTYNRFKFSYVHGWLRDDRDTVNGRKDLSDRKYIVAHRVEWKALPWLFLSG
                     SESVIYGGRDVELSYLNPLVPYHVAEQYLGDKDNNTMSFDVLAFLPGRTKVYGAVYLD
                     DFTSARNPFTYWKQTWAVMGGGYWVEPMGFKDAGVRIEYSRIEPFVYAHKWRLLNYTH
                     FDAGLGSQLQPNSDRWNFLISYQYSRRLRAEVGFRRIRHGEGDIQRFGYEYGFTDDDS
                     GKQKKRFLMGIQEIRNSALFNASFETWSHHALYLQYELSMIRNLGNRKGFDATQNYLR
                     IGYTLDY"
     gene            complement(15568..>16061)
                     /locus_tag="PK754_04700"
     CDS             complement(15568..>16061)
                     /locus_tag="PK754_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNB08834.1"
                     /translation="PVFFKQERWGRQNKKIICYKFRTMISQSRDVDDSGKYQQATQDD
                     PRITPLGRFLRKTNLDELPQFWNVLMGQMSVVGPRPHPTPLNMESKEVIENYMLRHLV
                     KPGITGWAQVNGYRGETRDPELMQKRVNHDIWYIENWSFWLDIQIIFLTVWQMIKYDT
                     KGY"
BASE COUNT         4287 a         4038 c         3456 g         4280 t
ORIGIN      
        1 tggcggtgta atgatgatca tcatcgacgc accgtcttca ttccatacac cgtcatgatt
       61 ggatgctttt actcgaaaga tatattcacc cggatcaaga ttggtgtaac tcacataccg
      121 gcgggtgccg ctgtacaacc atgttttgtc aaacccttcc agtcggtaag catactgatg
      181 ttgctccggc gcatgataca taagggctga aaattcaaaa gcaaatgcgt tacccgcgta
      241 cggcaatacg atctgctgcg attccgtgac ggattgtttt agcacgccgt tcggatcaac
      301 cgcgaccggt tgattggcta tctgaaactg cgtaagcaca acagataccg gcatcgtatt
      361 atccgtaatg cggtcaggat gaaaaacgtt gaacccttgt atcccgccaa aaaacatttc
      421 accgttttga tttttaaaaa atgcaccggc attaaattca ttactctgca gaccgtcttt
      481 ggtatcgtaa ttacgaaaaa ccgaggtttt cggatcaaac ttggacagcc cgccattggt
      541 actcatccat agaaaaccct tttcgtcacg gagtacgcca tacaccacat cgttggccag
      601 gccgtcgcgt tcacgaaaat gttgtgtaat acctgtgcgc gaatcgaacc ggttcaaacc
      661 gccggctgtt cccagccaaa atatattttg cgtcgtatcc ggcaatatgc aaagtacgat
      721 gctattgctg aggctttgcg gattagtcgg atcataatta taaaccgtta cagattgccc
      781 gtccgctgat atacaattta acccgccccc ttgcgtcgcc acccagactt taccgtcgct
      841 gtcctgatag agcccgcgaa taatgttaaa actcaaaccc ggcttggttt gcgggctata
      901 ctgttgcaaa atttttcccg atttttcgat gcgaaacaca ccgttattat tagtacctac
      961 ccagaatata tttttatctt tgcggtcttc gatcacataa cgaataaatt tgatattcgg
     1021 attggttgaa ggttcaaaca agccgcgatc cgtatccatc cgttgcaaac cggatttatt
     1081 ggttcctacc caaaaaacac cttcactgtc tttgaacaaa aaacgaacgg aattatcacc
     1141 gatcgtatgg cgaactcccg gcgtattgac atacgaacga aaggtctgtg ttatgcgatc
     1201 ccagcgatta agcccgccgc cattggtacc gatccaaagt acttttcccg aatcagccgg
     1261 atcttcaaaa atagaataca cactattgtg gcttaggcta ttggtatgat ccggatcatg
     1321 tcggtacgac acaaaacgta tgcgttgcgg atcgtatttg ctaattcccc cgtcacgatg
     1381 cccgatccaa attacaccgg tacgatcttc tgtcaaacgc aatatcaggt tattgcttag
     1441 gctgtgtgca tcgcctgact ccgttttgaa ctgcaaaaac ttgttttgtt ccggcgaaaa
     1501 aagaaataat cctccgcctt cgctgccgac ccagatacgc cctgcacgat cacagtataa
     1561 cgtctgcaaa tcatcttcgg taaggccgtg tttcgaggcg tctgcttttt taaaattatt
     1621 ctgaaggcgc caaacttcat catacatata tagaccacca ccgtacgtcg caacgtaata
     1681 ccaaccccgt cggtctttta ccaccgatcg cacactgttt tccgacaaac gatagtcacc
     1741 ggcagcttca cgatgataac gtacgacgtg gcgccgggta ggatcacaaa tgtataaacc
     1801 cgaattggtc gaaagaaaaa aacggtttcc gttttcaatt tgcaaatttt gaaccgacga
     1861 catgtcatcg gcagaacgcg gattccaccg ttcctgtatc ttcccgtttt catctacgcg
     1921 caggagcatg ccgcgatccg ttgtacacca taggtgccct acgctgtctt cggccatatt
     1981 ttcaatacga taccccgcga tggtcgtcgt atcgtcgcta cgcttgaatc gtaaaaattt
     2041 ttcatcgcgc ggatcgtagc gatttacgcc ttccaaagtc atcacccaaa tgaaccgacg
     2101 acggtcttcg tagagagtgt tgatgtaatt attggataac gaggtggaat caaacggatc
     2161 gtttttgaaa atgcgaaatt gatacccatc ataacggttg agcccgtcct ccgtaccgaa
     2221 ccacataaaa ccgcgacgat cctgcatcat cccgaaaatg gtggtttgcg ataagccttg
     2281 ttcaacggaa attcgctcta catacaaacg ttctgtctgc gccgctaaaa aagtaacgtt
     2341 catccacaac agcatagagg acgcgaattg aaaccgtttt gaaatacagc ctgtattcat
     2401 gttctgttta caccatacca tgagtcggtt ttgaactagt tcacgccgat acgttcggca
     2461 agtttttcga tatgacgcgg cgtcgtacca caacaaccgc cgattatttt taatttgtac
     2521 tccgataccc acgcttccat acgcgaagca aaatcctcgg gtgtcatcgc aggcttagat
     2581 tgttccggaa atatattgac ataagcgccg atcgggatat caccggccgt atctttcaat
     2641 acatccagcg catgttccat gatttctacg tgcatgcagt tgagcatgag tgcagcggga
     2701 ttatacgttt tgatacgccg tatggctttt tctaaagatt caccgctgag caattgcgta
     2761 tcattgcgcg caataaagct gacggcaacg ggtaatccgg tctgaacggc atattccgtt
     2821 gcaatggaag cttcattcat attattcatc gtctcgatca aaatcagatc cggccgcgct
     2881 gcatacagat catcaatacg ccgatgatgg tcatcgcgta aaacatcatc cgccggtgcg
     2941 cgttccggat gataacaatc ctccaccggc ccgatcgatc cggcgataaa aaccgtacga
     3001 tcccgggcta cactttcgat cgcttcgcga accgtatcta ccgctttgca gataagcctt
     3061 ctcgaatcat tatggagttg ctccgtcatg agcgtttggc gatttaggcg aaacgtattt
     3121 gctgtgatga tctgtgcacc gcgctgtatg tactccgcgt gaatagctcg taaaacggat
     3181 gtgtactcat tttgaaggag tgccacggcg gaccacaacg gtaaaacaca tggaacgccg
     3241 cgacgttcca attcagaacc cgtcgcacca tccaagagca gtggcttcgc atcccgaagt
     3301 cgttcagtcc aagccgtcat aaaataaata accgccggct gtgcaccggc ggtagtattg
     3361 gaaaaggttt acaaattttg taaagcggaa agattggatc gaagtttttc tacggtcgcg
     3421 cgaaaattat ccaatttagc gcgctctttt tcaagaacct gtggcggcgc tttggccaca
     3481 aaaccttcat tgcctaattt ttgttcgata ccaccgatct gacctgaaat gcggtcaatc
     3541 tctttttgta aacgtgtacg ctccacttca agatcaatca caccctctaa cggtaaatag
     3601 acttccgtac cattcacaac tgcactcgct gcgatgcggg gtttatccgc tccgacggcc
     3661 gccgtgattt tttctacccg cgcaagtccc gcaagtgccg atgtattagc attgtacagc
     3721 gccgcatcct gaggtgtaac acaacgcaga acgagatctg tttttttgga tggtgcgata
     3781 ttcatctctg aacgaatatt acggatcgta gtaatcgtat cccgcaattg ctccagatct
     3841 ttttcagctt ccacgctgat gtaggacgct tcgcactgcg gccacggttg gatcataata
     3901 ctttcaccgt cggtgcgcgg catcagacgt tgccatattt cctcggtcac aaaaggcacg
     3961 atcggatgca tcaaccgcaa tacaccgtcg agaatataca acgccgtgga taacgttttt
     4021 tctttgatct ccggagtggc attgtacata cgatctttga tcagctcaag ataccaatcg
     4081 caataatccg cccagaaaaa acgatagacc gaatccatcg cttcgtgcag tttgtaattt
     4141 tcgagtccgg tagtaatacc ttcaacggca cgatgatatc ggctcagtat ccaccgatcg
     4201 gcgagttcag tgatcgcgtt cgaaggcggc agttgcggtt tagcgctagg tagctgctcc
     4261 tgatagttcg ccaaaaaacg aaacgcattc catactttat tggaaaaatt gcggccgagt
     4321 tcaaattggc gttccgcgag attgatatcc tgaccttcgg cactgagatc aacgagcgta
     4381 aatcgcaacg catccgccga atatttatca atcacggcga tgggatcaat gccgttaccg
     4441 agcgatttgc tcatcttgcg gccgagttcg tcacgcacga taccattgaa ataaacctga
     4501 caaaacggaa ctttaccggt aaactccaac ccggccataa tcatacgtgc aacccagaaa
     4561 aagataatat ccggaccggt caccaaaagg ctcgtcggat aaaatttttc cagatctttt
     4621 gtttgatgcg gccaacccat agatgaaaaa ggaaccaacc acgaagaaaa ccatgtatcc
     4681 agtacatctt cgtcctgacg taaatcggaa accgttagct gcgcattttt tttctttagt
     4741 atcgccaagg cttcatcggc attacgtgct acggcgacac ttccgtccgg cgcgtaaaat
     4801 gccggtatcc ggtgacccca ccaaagctga cgagaaatac accagtcgcg aatggaggtc
     4861 atccaatgct catacgtttt aatccacttc tccggatgaa accgtatatc acctttaaga
     4921 acggcctcca gcgcaggttt agccaattcg tccattttca gaaaccattg ttccgaaacc
     4981 aacggttcga tgggcacatt tccgcgctgc gaataaccga cgcgatttaa gtgatcctga
     5041 attttttcga ccaatcctgc cgattctaaa tcggcaacca cagctttgcg tgcggcaaaa
     5101 cgctccatgc cgcgatattt ttcaggcacg ttttgattca tggtcgcatc ggcatgcatg
     5161 atattaagaa actgcagctg atggcgtttt ccgagtacaa agtcattcgg atcatgggaa
     5221 ggcgttactt tcatcgcgcc ggtaccgaat tctttatcca catagtcgtc ggcgatgatc
     5281 ggtatttcac gtcccaccaa cggaagtatc actttttttc cgatcaggtg tttgtaacgc
     5341 gcatcatccg gattgacgac gacggcggta tcacccagca tcgtctcggg gcgtgtcgtg
     5401 gcaatgacga tatgttcggc gctgtctttt acaggataac ggaaataata catctttccg
     5461 ttgatctcct tgtattccac ttcttcatcg gaaatagccg attgcgaata cggacaccaa
     5521 ttgacgatat actgaccctt atagatccag cctttattgt aaaaatgtat gaatacttct
     5581 ttgacggcct ctgatagttt ttcatccatc gtaaaccatt cgcgctccca atcgcaagaa
     5641 caacccattt tttgcaattg tccgacgatc cgcgcgcggt acgtctcttt atgcttccac
     5701 acttcattga caaaggcttc gcggcctaat gtttttcttg aagtcccttc tttcgctaat
     5761 tgtttttcga cgacggtctg cgttgctatg ccggcatggt ccgtgcccgg ggtccacagc
     5821 gtttcaaatc cctgcatccg tttgaagcgt gtataaatgt cttgcacgga attgttataa
     5881 gcatgcccga tatgcaatgc acccgttaca ttgggcggag ggatgacaat agtaaaaacc
     5941 ggtttagcgc tatcggcatg ggctttaaaa aaatcatgct tcagccaata cgcataccat
     6001 ttgtcttcag aggctttggg atcgtaagct ttcgggattt cagtcttttg ttgtgcttcg
     6061 ctcatagtaa aaccgacgtg tacctgatga ataatgaatt atttttgtgg agtgaatata
     6121 gaagaacggc atgacaaata aaagcgggtt ctacagcgta tttctgttgg ctccagaata
     6181 ttacccgatc atcccggaca ccgaataggc acgtgagtaa taattcgagg tgtccgtatt
     6241 aaaatttatc atcgtcaagc cgcactaaaa aattacttag tactttgcga ctttgtgtga
     6301 atattgcgca ccatatagta gtatccccaa tatcaggaca tctagtagaa acgaaagcga
     6361 acgcgggtgg gtcattattt attgtttcta gacggcgaaa cccatgcatt gccatcaata
     6421 aaataacgcc atttcttctc cgtccctacg cgaatgccga tacgcggcga cgtttttatt
     6481 tttgcatggt atcggtcatc agaaagatac agatcgtcgg tcgtcagatc ggttccatta
     6541 tgggatttgt caatgcccag cgctaaacat agtttccctg gtccgttact caaagcaaag
     6601 ccgcgagcca taacacggtt agcttgcatg cgttctacgc catcaagcgg ttcaaccgcc
     6661 cggatcaggc atgcaccggc tgtaccttct ttttcgcata cgacattggc acagtaatac
     6721 atcccgtaaa taaaatacac atacagatgg ccgggcacgc caaacattat tttatttcta
     6781 ggcgtcaaac cgcggtacgc atgcgacgcc gggtcatcac cggcacgata agcttctact
     6841 tcgacaatac gcgccgacat cgtcccgtga tgcaaaattt ttcctaataa ttcgggagcc
     6901 acttctaagg taggccgcgc ataaaacgaa cgcggaagcg gtttaggcat gatgggtttg
     6961 tttgtcgtgt ttaaaccctt gatgaatttc ctctaataga aaatccacct gtaacataaa
     7021 tcgttccgat gcacaaccgc ggcgcttgca ctcacggcag gggattttca atttacaatc
     7081 cacctcgttt ttatgcatgg tttcgatctc gccgaacgct tcattgagat cgctttcgtt
     7141 ttcttcctgt ttacgtttcg gttcgttttc cgtcatcaat ttaccccatt tgatgcgaag
     7201 catctcttca aggtctttga tacggccaag aaactgactg atcgtattac gccagatcat
     7261 cacagcttta ttgagtacgg cttcatccac ttcgtcttca acaccggatt cttcttcaaa
     7321 cttgaaactc ttcagatcaa gaatgtgtcg caccatacct tgaatctctt caaattccgt
     7381 aaaaagctca cgtttggtat cgctatagag cgcattgatc gaaccttcaa aatgctcgat
     7441 cgcttggcga atcacgccct gcgcgacggt tgctgatccg gattcgagat ttttggcttc
     7501 tttggcaaaa agctgaagat aattcagtga accgcgcaac atagtgacag cttctttttg
     7561 cgaattgata cgactgtaat tgagagactg aaagatcacg tcaaaataag cattaacgct
     7621 gacttcgatt tccttcattt ttttatttaa tcgttcggct tctttgagac gaatctccgt
     7681 ggtacgttga cgcactcttt catcggtgct cttttcaagc atcggcaaca cttgcgatga
     7741 aatatgccgt ggcgtcgaat catcggatac cagatcaata atatcatccg gctgcaatat
     7801 ttccaacgcc tgcgccctca gatcggcatc tttatcaaaa tgaagatagg taatcagcgt
     7861 agcattaaaa agatcggtcg ggtcttccac gcacttttct aactctcttc gtttgcggtc
     7921 ttgcaatacc tgtgtgagtt gccgacgtgc ggcattgtgt tcattcgtct gatccgcccc
     7981 cagatcaccg ccggcagcca ggttacgaat ttcaagatga accgcagact ccagcatgcg
     8041 aaaaatcgaa atcgccgtta ttttggatat ggattcccgc atggtgtgta tgagcgaatc
     8101 atcttcgagc gcattgatca acgtttgcac atccaatgca gagacgacat tttctgcggc
     8161 cattcgcact tgcgcatcat cgtcaatgag gtatgaaaac aatgtaatca aattagcacg
     8221 ttgtatcggc tgcgtattgg ccgtatgtat ctcacgaact ttgacgatac gacgactgcg
     8281 ccgcttcatg accttttgtg caagcttgag gattttatca tcttcgcgat cgatgtcgcg
     8341 ttccttgatc agattgataa aatcatacaa taccttggtg ggcaactgag gattaccaaa
     8401 aaccgcttcg agaacatcca ggtcgcgttc aaacaacaaa aacacgatca aatgttgtac
     8461 ttcgttttca cggccaaatt tgatgcgctc ctgttcacta aaatccaaga tgtgaacata
     8521 ttttcctacg tcgcggaaaa acggatcgtt tttcatcgca atacgcgcat cgggcggttg
     8581 ttcatctaag tttttatagg attgaatatc aaacggaagc gactgacgat aataacgatg
     8641 caggatttct ttataatgaa catgatccga ttttgaaaat ttatcgtccg tttcgatcag
     8701 acgttccgga tcggtgatat cctgtgtatt cgttatttta gtgttttcag acatgatgtg
     8761 ctgtttcggt tacattcgaa tggtttaact ccatcacggc gctgatcgta tgctgatgcg
     8821 cgacatgtac ttggatttgc tccttcatcg cggtagtttg caaatgttct gtcatggacg
     8881 ccatcgcaag atccggcata ttattatgtt cactcagatg cgccagcaca agccattgca
     8941 gccttgggaa catcgtatcg cgcatcaatt cgccggcctg acgattggaa agatgaccta
     9001 cacgcgatgc gatacgctgc ttcagcggcc atggataacg tcctttcatc agcattccct
     9061 cgtcgtgatt ggattccaaa atcaagatat ccgaatcctg aatgcgtgct ttcaccagtt
     9121 cggatgcata ccccatatcc gtagcgacgg tcacttgcct atggccgcat tggaacgaat
     9181 aacctaccgg ttccgtggca tcgtgtgaaa tggaaaacgg ctgcacctga agcccgtcgg
     9241 cttcaaatgt atcggaaaac acatgcaccc tcgtctgctc cggaatgcga tgtctcagat
     9301 ggcgaaacgt tcccgcattg caatgcacca taccgctaaa acttttcaat atttttttca
     9361 gacctttgat atgatcatcg tgttcgtgcg tgataaaaat gtgttgcagt tcgttgatcg
     9421 gcgtagcgac cgccgtaagg cgatccatcg tttcttcacc gctcaacccg gcatcgatca
     9481 gaactttagt tgcaccgtac tgcaaaaacg tactattgcc tttgcttccg ctgccaagta
     9541 cgcatacgcg caccgcattg ttcgcttctg ttaacacact atctccgttt agggaaatta
     9601 aactgaaacc ggggctgtag tcaagaaatc aattttttga gaaggtcttc ggcttatttg
     9661 ttcatcgaac ggcttccggg cttgatcgcc ggcaatgcgc cttcccggca taggggacat
     9721 gtttcaggtt catatttgac tacatccaca gagactaagg aaaatttagg tacgccaaac
     9781 tcggcacgcc cgccgctgcg atccaccaga taaccgacgc cgacgacatt cgcatccgca
     9841 tcacggcaaa gttgaatcac ctctttgagg gaaccgccgg tcgtcacgac atcctcgaca
     9901 accagcacgt tttcatcgcg tttgatttca aaaccgcgcc gaaaacgcat ggtgccgttt
     9961 tcacgttcgg aaaatataca acgtgcattc agagtgcggg ctacctcatg cgctacgatg
    10021 atcccgccta ctgccggtgc ggccacggca tggatttttt tatctttata atgcaatgct
    10081 atttcactgc acaacagctg agcccagttg ggatgctgta aaacgcgagc gcattgaaaa
    10141 taattaggac tgtgtgcgcc cgaagtcagg ataaaatgac cttcgagtaa agcgccgctt
    10201 tgtttgaaaa taccgagtac ttcttcctgt ttcatcattc ggttgtatag tttttagatg
    10261 agctgacttg ttcgatataa ttccacacat taaaaaaatc atgcgatccc gaataataaa
    10321 ttcccaaagc cgtacacaaa acgatcagcg ccccgcctaa aataaatccc gtacgccacc
    10381 aactcgcatt aaaaagtatt tgcgctgcgc ggaaaaaacg aatccaatgc caaaagaaaa
    10441 acaatacggc cgccgttgcc cccaggctca aaacaaaatc ggttttcatg agacgatgga
    10501 agaccaccac aaatggaatg agatacagaa aatgcgaact gctccacgcc gccgcaataa
    10561 tactttgctg gaaatttacg tacgcctgaa agggtttttg aaatatcata aaaagaagtc
    10621 cgatcaccgt gatgaccaaa gcgtaaatca tcgtccactc cagcacaaaa agttcaggac
    10681 tccatgccaa gccgtccacc caggctttga gaccattgct gcgcaagaat gtcgcaaggt
    10741 actcatcaaa caaatggttc cgccgcaatt caaagaaaac gatagacagc aatgccgcat
    10801 acgccaaact cgcaagaaag ccgactaaca acgccggcca cagtgtgatg acgcggtttt
    10861 cgcgaatgtc accaaaaaag cttactactt tgaacaacga acggtgaacg ctgtttttaa
    10921 atttaggact ttgccgataa aaactgataa acaagacgac caatatcagt cccgtgatcg
    10981 gatacgtttt gggatatacg ggttcgtagg caccgacggc gatcggttcc aaggcatcat
    11041 ccgaatacaa ggcacgcaat gtttcaaacg catagcgctc ctgacgatcg taagtcacaa
    11101 ccccggacgt atagatcgtt gagctcgcat tcggattggc ataaaggtac gggcgctgta
    11161 cggcaaaatc attgaacgaa cgcactacgc cgccggccaa tttccactcg ccggtctttt
    11221 tataaaaatc agccaacacc ttagcctgaa atttggtgga catcgggtcg ctgtagccat
    11281 tctgattatt ggaaaaaatc tgcgcaccgt acaccgcgat caatgcacgt gtgggccact
    11341 tggccagact gcgctggacg aaaagattgg aagacagagg actattgagg cgaatatcca
    11401 gctggacaat atccacattg tccataaacg aatcgtagcc gccaaaagcc gaagcggcga
    11461 aaacgggacg gcgatcttta ttttttattt caccgacaat ttgctgaaag taataattgg
    11521 ctgcgccatc cgacggatcg ggagcaaatc ctacacccca ggcaagaata ctcggattga
    11581 gatgctcata ttcgacgatg tgccgatagt atttgcgaat atcttcgacg taactcggtg
    11641 tacgcataat atccgccgta agccgccagg atggaatttc ataaaacaaa agcaaaccat
    11701 aacgattgca cagttctaca aaatacggat gcggcggatg atgacgaaaa ctcacggcat
    11761 tagcgccaag gcgtttgatt ttgagaatat cctgctccat catcgcccag ctcatggtgc
    11821 tgccggcgcc cggatagtct tcataatatt cgaccgcgcg tatcgtcacg ggttgcgcat
    11881 tgagaaaaat atactgatcg tgcacatcgg cttgagctat accgagttgg tattcgacac
    11941 gatcgatcac cgtcgtgccc tgtactaaat acattacaac gcgataacga ctcggcgtga
    12001 tcgggttcca aaagaccggt ttttctattg aaaaacgtaa ttgacgatca acaaaattga
    12061 aaaccgaaac cgtatcttct tcgttttgct cgcgaatttt cggaatgtcc cacacgttgc
    12121 gatatttttt aaaccgattt gtgtacaccg gctccggatt attttcctca aacatttcca
    12181 aaatatagcg caacggctga ttggggttga tcaaccgcaa tgtgtccaca aggcgcaaac
    12241 tatattcgta attacgcaaa tgccaattga ccacaaaatc acatttgcgc aaatcattgg
    12301 acaacgtata acctacggtg tattgatcaa tagcaacttt tggaagacct acgagaaaga
    12361 gttcacgggt aataccgccg tacaatttgg gttgcaataa actggtttca cggggatagc
    12421 tggtaaacgg atcaacacgc gaatccaccg taatttcaat tttgttttcc tgaccgatac
    12481 ggatcaggtc ttcacgcaag tccgcttcca atcgcgtata actgttattg gcattggtga
    12541 caaattcgcc attgatgcgt atgtcggcgc gaaaattgat acctaagctg acaaaacgaa
    12601 agtgcttatt acgaaaatct tcgccgggaa cgaacgtacg cgtaaacgta actttgccgt
    12661 tataatcata agaactggga acaatgactt ttcgtgaaac tccttccacg gacatggtcc
    12721 actcacccgc caagtctaaa aaggcgcggg tttcggtcag attgacatac tcgcgtaagg
    12781 tctggggatc atagccgttt ttttcgccga ggacacgaaa atcattttgc gcttgaacga
    12841 aaagcgcgca gcatagaaga gtgatgatac gaaaaatcat tcgcagtact gctccggtcg
    12901 cggccgcaat ggccgaattt acataattcg tttaccgaaa agtgaagcca tcatttcgac
    12961 gccgacatgt gccgtcgtgt ttttaatatc aagtatcgga ttggtttcaa caatttccgc
    13021 agaaattact tttttggaat cgcacaggga ttccatcgcg aggtgggttt cacggtacgt
    13081 aagtccgccg ggtaccggcg ttccgacacc cggagcaata ctcggatcca tactgtccgc
    13141 atcaaagctc acatgaatgc ctgcggtatc tcgcgcggct atatcaatag cttgtcgtat
    13201 aatcttggac ataccttgtt cgtcaatgtc gcgcatcgta aaaacggtga cccccatacg
    13261 tcggatatta tcgatttctc cgtcatccaa tgtacgaata cctacaagta cggtattctc
    13321 aggattgact ttgacaaatt caccgccgac accggtcaac ttgggatgtc cgtgtccgag
    13381 tgatacggca agcggcatgc cgtgaatatt tcctgaaggc gaggtatcgg gcgtattcat
    13441 atcgccgtgt gcatcgatcc aaatcacacc gatcttctga ttgttttttc taaaataacc
    13501 ggatacgccc gccagtgtgc cgatagcgat ggagtgatcg ccgccgagta caagcggtat
    13561 atgattatcg gcgaggcatt tttcgacgtc atcggccagc gccgttacgg cacgcgttac
    13621 ttcattgagg tacttgagtt tttcgtcgcc gaccctaatg acttcagggt ttttggtaac
    13681 gatatccccc ttgtctttaa cggtgtgacc taattcctga agtcgctccg tcaaaccggc
    13741 caaccgcaaa gcggacgggc ccatatcggt tccgcggcgc cctgcaccca aatccaatgg
    13801 aacacctatg agacgaatga cggacataca ttacctctga aaatttgaat tttaaaaccg
    13861 cgcgaatata cccgtcccgt gcctaataat caagcgtata accgatgcgc agatagttct
    13921 gcgtcgcatc aaaaccctta cggttaccca agtttcgaat catagacaac tcatactgga
    13981 gatataaggc gtgatgtgac catgtttcaa atgaggcgtt gaacaacgcc gaatttctaa
    14041 tttcttggat acccatgaga aagcgtttct tctgtttacc ggaatcatcg tcggtaaaac
    14101 catattcgta tccaaaccgc tgaatatcgc cttctccgtg gcgtattcgc ctgaaaccga
    14161 cttccgcccg aagacgacgg gagtattggt agcttattaa aaaattccat cggtcggaat
    14221 tgggctggag ctggcttccc aaaccggcat caaaatgcgt ataattcagc aatcgccatt
    14281 tatgcgcata cacaaaaggt tcaatgcgtg aatattcgat gcgcacaccg gcatccttga
    14341 accccattgg ttccacccaa taaccgccgc ccataaccgc ccaggtttgc ttccaatatg
    14401 taaatggatt tcgtgccgat gtaaaatcat caaggtacac agcaccgtac actttggttc
    14461 gtccgggtaa aaaagcaagc acatcaaaac tcattgtatt attatctttg tcgcctagat
    14521 attgttcggc gacatggtaa ggcacgagcg ggttcaggta tgaaagctct acgtcacgcc
    14581 ctccgtagat cacgctttca cttccggaga gaaaaagcca aggtaacgct ttccattcca
    14641 cgcgatgcgc aacgatatat ttccgatcac ttaaatcttt tcgtccattc accgtatcac
    14701 gatcatccct taaccaaccg tgaacataac tgaatttgaa tcggttgtag gtaacatcca
    14761 atctcaaatt ttcaaacgaa ggggcattgc ccgagagcaa aagattatca taatatcccg
    14821 gcccccacgc catattgtct tttccgactt gcaggcgaaa ccattttggc tcaataacga
    14881 tatatgcatc agagtgtaac gcataacgat tagcgctggc cggattatag ttgagtacac
    14941 cgccacgata actgctacca aaatcggtag attgttcatc tcctttaacc aatgtattgg
    15001 acgcatcggc ataaaacgca atgccgcctt ttatcaatcc tcggacacgc ccactacctt
    15061 tggaaaggcc tatcgtacca cccaatgaat catttttttt ggagaatcgt gtcaattcga
    15121 cgctctgatc aataaccagg tcacccacca aataactgaa tccgtcttcg tgcgtttgat
    15181 attcaaaaat atgtttctca cgcacatctg atgtttgtat gttctgcgta gaggttagtt
    15241 ctacataaaa ttcgcccttg agcttttcaa ataaactacg ttcggccgat gtcaaaacat
    15301 tcgcatctgt tttgattttg tcctgcactt catttatgat tttaagtacg gttccgcggt
    15361 catacggttt ggttcgaaga cgaatggatg gcgatacaac accccgtgtc tcaagatgct
    15421 ctatcaaatc gtatccgaag tgccccatcg ggaggttaac agacggccct gtatattgcg
    15481 cccatcctgg gcagggcatt aaaaaagcgg ctacgagcac cgccaatcct gtcgcaacta
    15541 tgtacttttg agaatgaagc gccaacatca atatcctttc gtatcgtatt tgatcatttg
    15601 ccatacggtc aaaaatatga tttgaatatc gagccagaac gaccaattct caatatacca
    15661 aatatcatga ttcacacgtt tttgcataag ttccgggtca cgggtttcgc cgcgataacc
    15721 attaacttgt gcccagccgg taataccggg ttttaccaga tgacgaagca tataattttc
    15781 gatcacttct ttggattcca tgttgagcgg ggtaggatga gggcgcggcc cgacgacgga
    15841 catctgtccc atcaacacat tccaaaattg cggcaattca tccaaatttg tttttcgtaa
    15901 aaatcttccc aatggtgtta tccgcggatc atcttgggtc gcttgttggt atttcccgct
    15961 gtcatccaca tcccgacttt gagaaatcat tgttctaaat ttgtaacaga taattttttt
    16021 gttttgccgt ccccagcgtt cctgtttaaa aaacacaggg c
//
LOCUS       DAOQNQ010000044        15630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1341_length_15630_cov_5.943628, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000044 DAOQNQ010000000
VERSION     DAOQNQ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15630
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1341_length_15630_cov_5.943628"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="PK754_04705"
     CDS             <1..971
                     /locus_tag="PK754_04705"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNB08835.1"
                     /translation="YTMTQESETPGRWAWLSNPWVNGILFVITFAVVFMTGAATITNR
                     SLSDDWDWVMGFQFSFSLLGILTAHEFGHYFAARYHKLNVTLPYYLPGILIPPIPWLG
                     FPGTTIMPGTFGAFIRIKSPIINKRQLMDVGAAGPIAGFVVCLFVLIYGFATIPDKTW
                     AYQFYNVEEIFSGQPVMYFGDSLLFRWLGHGLAGDRMPAMYDIVHYPFIFAGWFGLLV
                     TALNMLPIGQLDGGHILYALIGSKQRFAAYGAFAVIITIGLFYGAGSWFIWALLILFL
                     IRFKHPRVMDEDEPLDNKRIAIGIVSFLIFFVSFIPLPVYEKIPIP"
     gene            989..1834
                     /locus_tag="PK754_04710"
     CDS             989..1834
                     /locus_tag="PK754_04710"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNB08836.1"
                     /translation="MRYHSRFDSYNRWLGTDYPMVKLLISINVVVYFIQYILIFFGLG
                     RWVSMHLALSPYIWDGEIWQLVTYMFLHSANDPFHILFNMLALWMFGKELEMVWGSRE
                     FVKYYFICGVGAGLTFLFFSNGIVVGASGAVFGILLAFGMTFPDQIIMMSFLFPIKAK
                     YMVMIYGVITFMNIANPSGDNIAHLAHLGGMVFGFLYLKKEWFKQKMQETPKPRAAER
                     PPVTPPVMTVQSRPKPPEPKQVIRDRVDEILDKINEVGYDKLTTEEKEILLRASQNYA
                     EQKKP"
     gene            1831..2652
                     /gene="pyrF"
                     /locus_tag="PK754_04715"
     CDS             1831..2652
                     /gene="pyrF"
                     /locus_tag="PK754_04715"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNB08837.1"
                     /translation="MSFRDRLQQAMQLSPVCVGLDTDMRLIPEFLHSAPNPILEFNCR
                     IVDATIDLVGAYKPNFAFYEGHGVVGMEALRETVAYIRAKNPDIITVADAKRGDIGNT
                     AAMYAQSIFEDLEFDCVTVNPYMGWDSVSPFVKDPEYGAFILCLTSNSGSHDFQYKMI
                     DGKPLYRHVAEKSQLWNTSGNIGLVVGATHPDDMKSVREAAGDMPFLVPGIGAQGGDL
                     ASVVQSNASGETLRALINSSRNIIYASNGKDFEDAARKATEYLRDQIGKLLQSGV"
     gene            2743..2817
                     /locus_tag="PK754_04720"
     tRNA            2743..2817
                     /locus_tag="PK754_04720"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2778..2780,aa:Val,seq:tac)
     gene            3283..3582
                     /locus_tag="PK754_04725"
     CDS             3283..3582
                     /locus_tag="PK754_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08838.1"
                     /translation="MKTAQRDSAALKDLIMLSWRNRKEGMYRGSAIGFATGAVGSVAV
                     TKLGYSGVPFSAGDYFLIAISNGLLGGLVGIPAGLLFGKKYEIYPCLPNSKNDCK"
     gene            3912..6737
                     /locus_tag="PK754_04730"
     CDS             3912..6737
                     /locus_tag="PK754_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HNB08839.1"
                     /translation="MPSEKSKSQGKKTSSTTPKTMIFGVEDAKKLREAIAANPTVNPS
                     PNEADAKTAESKETAEHKRMKEFTPGVPPWRRWGPFVSERSWGSVREDYSKDGNAWAY
                     FSHDHARSKAFRWGEDGIAGWCDRYQVVTFAPAFWNGKDPILKERLFGLTPLEGNHGE
                     DVKEYYFYLDSVPSHAYNKYLYKYPQAEYPYQWLIDENKRRQGQGEEFELLDTGLFDE
                     DRYFDVFIEYAKAGPEDILIRVEVHNRGDQDAPFHYVPNYWFRNRWGWSKPRTGEPTI
                     VPGPKGKNFVSVISDDTTMETFKNLPFLYQLGKRYLYAEGNPEIYFTDNESHHEKLYG
                     VPSAKPHVKDAFHRYIVNKENCINPDQVGTKSCFHYTDTIPAKSSKVYRLRLTAETLQ
                     SPFEKFDEKVRERQNEADEFYETVYPPKLSADERNVMRQAYASLLWTKQIYLYDVNRW
                     LTGDDPTMPPSESRWTIRNHHWRHLNSMRIISMPDKWEYPWFAAWDLAFHTIAFADLD
                     IEYSKEMLWIMLFEQFQHPNGQIPAYEWEFSDLNPPVHAMAVWKVYTMDRDRTGKPDL
                     VFLEKCFHKLLMNFAWWINKVDASGNNVFEGGFLGLDNITVVDRSEKLPGGAVLEQSD
                     ATGWMGMFCLNLMRICLELARYNKVYEGLATKFVQHYIYVGAAMKNMGEQGIELWDEQ
                     DGFFYDVLVYPNHSGYEKFRVRSLVGLIAMYAIDILNAKDVEANKEFVSNLDWFVRNR
                     PDIVQRCVYIDETDASNRRHILSIVDAHQLQRIMERMCDPEEFLSPAGLRSLSKYHAE
                     HPYYFGEKKVEYEPAEATSKIKGGNSNWRGPIWFPTSYLMINALQKFSEAYGDDFGTT
                     RDGKLIRPKDLAQDIAERMIRIFTRDAKGHRPVYGDIKKFQNDPYWKDYVLFFEYFHG
                     DTGRGLGASHQTGWTGLIATLLQEWRR"
     gene            6738..10079
                     /locus_tag="PK754_04735"
     CDS             6738..10079
                     /locus_tag="PK754_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNB08840.1"
                     /translation="MNALWSLVLRLCFYFTCVASFLSAQTPTSGALNIFVQTGHSSQV
                     LGLDKTSDDRFLVTGSTDATVKLWEIASAKEVRTLRGHTAPVTHVAISPDGKTIVSAS
                     SKDSSVMIWESVSGVRKLKIRLNYEMTAMLVRPTWNSILVSSSEDAVIEYSLQTGKEE
                     RRFQNVGSLLCISDDGKKAVSRLYDRPDGVIVASAERLHIWDLLNGQTIQKLDSSNAM
                     TAAITRDTRYVVTGERNNTIKLWDIMAGRSVRTFGYETEPAMFASDEMDEVISIFFSP
                     DEKKILSAHKLSGYYVWDRKTGEPLRQLHADSYRKVDEMKAENGVVSIRACAFMKDGS
                     TIFGIDGRRIHMWNNDTTEPILRYQGVVQGRINDVRTTPDGGSLILANSENGLRFFDV
                     HSGKPAAAFLSTVSDIQSVAISNNGKFVLLVDQASVASLWDITAKQPLCTFKTEVPFA
                     ALSDDGEIAISANENEFAAYRTRDGVMLSTWRSPHITWSDITFIPNSRRVAIGFWKTP
                     YAPFDGLLTVWDIPSGQLVFELHSEAGTPRCVSVSFDGKMILSGHEDNTLKLWDSHSG
                     KLIRSFNTLIPLAFMKPYEGITAAGLSPDGRWTVSGSADGTIKIWRNDNGTEWRTLKG
                     HTGTIGLVCFSPDSKSAFTAGADGSVRMWDIESGTEYVSFVSLGDADWVTMTREGYFD
                     AGGAGARNINVSAGDQVYTIDNFFETYFRPDIVQAKLAGRAIETMGSSDGLLQGVKIP
                     PTLKLLALDHNGQYAPLVSENEKTANISNGVIRIKIIAEDAGGGVSGIRLFNNGKAVG
                     ENIRGFKATPSIEKIEQEFFVTLSEGENILAAVGFSNDKSESNPVKARLFYRRPVLAK
                     PDMHILAIGINQYRNAKYNLNYCVGDAQGFVNAITPKAKKIFSNIYTTLLLDREANRA
                     GVLAALASIQAAAKPEDVLIIFYAGHGMAMDIEENGPSRNEFFYILSEVTQMSDPMKC
                     AQEGISGTEMRKILSDIKATKQVMFVDACNSGAFAQQFAVRGAAEENALAKLSRASGS
                     VIYASTTKEQFATELAALKHGAFTYVLIDALGGQAGLPNGQLTVSSIKAYVDDKIPEV
                     TKKYKGEEQYPTIFIWGQDFPIGMK"
     gene            complement(10157..10522)
                     /locus_tag="PK754_04740"
     CDS             complement(10157..10522)
                     /locus_tag="PK754_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HNB08841.1"
                     /translation="MATKKPAKKKPAKKAAKKTVKKAAKPKKAAKPAKKKTARKPNAA
                     FMAKLTPSATLAAVIGDQARPRTEMIKKIWDYIKKNGLQDKTNKRMINADDKLRALFG
                     GKSQVSMFDLAKLVSKNVA"
     gene            10777..11220
                     /locus_tag="PK754_04745"
     CDS             10777..11220
                     /locus_tag="PK754_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HNB08842.1"
                     /translation="MKSVFDPAARQEIIDRVNKLSPATQPQWGKMNVAQMLAHCGISF
                     RVPTGDFTIQKTWVRFIGRFFKSLATNDKPFQKNSPTAAEFLITDERIFETEKKNFID
                     NFQKVSRGPEAITYFEHGFFGYLTAEEWGKMIYKHTDHHLRQFGV"
     gene            11222..11950
                     /locus_tag="PK754_04750"
     CDS             11222..11950
                     /locus_tag="PK754_04750"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HNB08843.1"
                     /translation="MIKNFCIACWLFSSVVFAQYNDSELYVTVLSQHVHSGKVDYARL
                     RLDRRFSNYLFSLTEVNPDTIQNLNARKAFWINVYNAYTLSVVRDHYPVKSIRAIDTG
                     LFGTSVWDKPYVHVGGKKYSLNDVEHKILRPMNDPRIHFALVCAAKSCPVLRNEPYRA
                     EKLDEQLNEAARQFMNDPERNRVDHQAKALYLSSIFKWYGTDFGKSTGDMVRYLLRYM
                     DINDTDRVVSYRVEYLDYDWSLNE"
     gene            11982..12668
                     /locus_tag="PK754_04755"
     CDS             11982..12668
                     /locus_tag="PK754_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNB08844.1"
                     /translation="MISIIIPTYNEIQNIPIRIEECSALTDAQIILADGGSTDGTAEY
                     VRDAAIRNNHLDWIQSGKGRALQMNAGARLASGAWFVFLHADTSLSQSSWEVLRKTIE
                     EKPQLIGGAFYLQMDHPGWKYNYLAWYSKQRSRLFKTPFGDQAVFVKADIFRALGGYR
                     EDFPIMEDLDFVRRLKAAGDFEMLPAYVVTSARRFAEEGFFRRSCKNLFMQTLYCFGV
                     SPSRLKRWYE"
     gene            13024..13881
                     /locus_tag="PK754_04760"
     CDS             13024..13881
                     /locus_tag="PK754_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNB08845.1"
                     /translation="MARIKMRINSREDQSIERYLEEINKVQLLKPHEEIELAKRIKKG
                     EKEALKKLVEANLRFVVSVAKDYQNQGLPLSDLINEGNLGLMKAAERFDETRGFKFIS
                     YAVWWIRQSILQAIAEHSRIVRLPLNRVGMLNKIGKAYNELEQEFEREPSTEEIAHEL
                     EIKDYDVSDALRISKRHLSLDAPLQEDEKSVLMDTVPDKVNPLPDETLMQESLRRDIE
                     NTLASLSERESKIIKLYFGLGIERSLTLEEIGEEFKLTRERVRQIKEKAINRLRHKSR
                     SKSLRSYLG"
     gene            13942..15276
                     /gene="dgt"
                     /locus_tag="PK754_04765"
     CDS             13942..15276
                     /gene="dgt"
                     /locus_tag="PK754_04765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HNB08846.1"
                     /translation="MVQETLHPQKVFFERGRQFVEDCEEQFLAPYACKSKDAHKTRQH
                     GEMEDVHRTAFQRDRDRIIYSNSFRNLSDKRQILVEHDSERNRSRLTHTVEVVQVSKT
                     IAKSMGLNEDLTEAIALGHDLGHPPFGHTGEAILDALLNGEDTAEGTLPGDDFGGFKH
                     NYQSLRVADILEKKYEYEGLNLTSFVREGILKHTSIRSERYRYPDIDTDKLHCDQFVS
                     LTLEGQVAAIADEIAQRTFDLEDALRANYIRLKDVRELKLVQDVDRSCSIAHFYEEDP
                     DEYINFMISGLVRVLVSDVIQNTIRRVEEFCGRKQRTDHFDEWLVIFSLDINRKQNEL
                     ETFIQKEMGNNFLVNRYNKKCEKVIRKLFKAYYSNPLQMNDKFLMRFTQKQVDAKFDL
                     RKLPPKMVQEKVSEFKKNPLYARHIADYIASMSDSFAIQEYKAIYIPEASVL"
     gene            15283..>15630
                     /locus_tag="PK754_04770"
     CDS             15283..>15630
                     /locus_tag="PK754_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08847.1"
                     /translation="MRLLKSHRIFFLSLCIAIFLWLYVRLGATYQMEIHVPFQVTNIK
                     DGYGVVSDIPEAVDILFEGDGKSLLGMRFLNPPRFVLDMAQHKDKSRFILSEHLHEIV
                     YPSKAPVHAIAVQW"
BASE COUNT         4135 a         3390 c         3884 g         4221 t
ORIGIN      
        1 gttacactat gacacaagag agcgaaacgc ccggccgctg ggcatggctg agcaatccgt
       61 gggtgaatgg catcttgttt gttattacgt ttgctgtcgt atttatgacg ggtgccgcaa
      121 cgataaccaa cagatcatta tccgatgact gggattgggt gatgggtttt cagttttctt
      181 tttcgctgtt aggtatatta acagcgcatg aattcgggca ttattttgcc gcgcgttatc
      241 ataagcttaa tgtgacgttg ccttattatc ttccggggat tcttattcca ccgataccgt
      301 ggttgggttt tcctgggaca acaattatgc caggtacgtt tggcgctttt attcgtatca
      361 agtcgccaat tattaacaaa cgtcaactca tggatgtcgg tgcggcagga ccgattgcgg
      421 gattcgtggt atgtttattc gtgctcatat acggtttcgc gactatcccg gataaaacat
      481 gggcgtatca gttttacaat gttgaagaaa ttttttcggg ccagcctgtg atgtattttg
      541 gtgattcgtt gctgttcaga tggcttggcc acggactcgc cggtgatcgt atgccggcga
      601 tgtatgatat cgtgcattat ccgtttatct tcgcaggatg gtttggatta ttggtcaccg
      661 cgctcaatat gcttccgatc ggccaattgg acggtggtca tattttatat gcactgatcg
      721 gatcaaagca acgttttgcg gcatatgggg catttgccgt gatcattacg atcggtttat
      781 tctatggggc ggggagctgg tttatctggg cgctcctgat tttatttttg attcgattta
      841 aacatccgcg ggtgatggat gaagacgaac cgctggataa caaacgaatt gcgattggta
      901 ttgtatcgtt ccttattttt tttgtaagtt ttatcccgtt gcctgtgtat gaaaaaatac
      961 cgatccctta aataaagaaa gttactgcat gcgttatcat tcccgatttg acagttataa
     1021 ccggtggttg ggaaccgact atccgatggt caaacttctt atttcgatca atgtcgtggt
     1081 atattttatc cagtacatcc tgatattttt tggattgggg cgatgggtat ccatgcatct
     1141 ggccttgtca ccctacatct gggacggtga aatctggcaa cttgtgacct atatgttttt
     1201 gcatagcgcc aatgatcctt ttcacatttt gtttaatatg ctggctctgt ggatgttcgg
     1261 caaagaactg gaaatggtgt ggggcagtcg tgaatttgta aaatattatt ttatctgcgg
     1321 cgtgggtgcg ggtctgacat ttttattttt ttcaaacggc attgtcgttg gcgcgtcggg
     1381 tgcggtgttt ggcatattgc tggcttttgg tatgaccttt cccgatcaaa tcatcatgat
     1441 gagcttttta tttcccatca aagccaaata catggtgatg atctacggtg tgattacgtt
     1501 tatgaatatc gccaatccca gcggtgataa tatcgcccac cttgcgcatc ttggcggcat
     1561 ggttttcgga tttttgtatt tgaaaaaaga gtggttcaag cagaaaatgc aggaaacccc
     1621 gaaaccgcgc gccgcagagc gtcctccggt aacaccgccc gtaatgaccg ttcagagccg
     1681 acccaaaccg cctgaaccca agcaggttat tcgtgatcgg gtggatgaga tattggataa
     1741 aatcaatgaa gtaggctacg ataaactgac gacggaggaa aaggaaatat tactccgtgc
     1801 gagtcaaaac tatgcggagc aaaaaaaacc atgagctttc gcgatcgttt gcagcaggcg
     1861 atgcagctga gcccggtgtg tgtcgggctt gatacggaca tgcggttgat accggaattt
     1921 cttcattcgg ctcccaatcc gattttggaa tttaactgcc gcatcgtgga tgcgacgata
     1981 gatttggtag gcgcatataa gcctaacttt gctttttatg aagggcatgg tgtggtgggc
     2041 atggaagcgt tgcgcgagac ggtggcctat attcgcgcca aaaacccgga tatcattact
     2101 gtggccgatg ccaaacgcgg tgacattggc aatacggcgg ccatgtatgc gcagtctatt
     2161 tttgaagacc ttgagtttga ctgtgtgacg gtcaatccgt atatgggatg ggattcggtg
     2221 tcgccttttg tcaaagatcc cgaatacggg gcgtttattt tatgcctgac gtccaatagc
     2281 ggttcgcacg actttcaata caagatgatc gacggaaaac ctttgtatcg gcatgttgct
     2341 gaaaaatcgc aattatggaa tacatccggc aatatcgggt tggtcgtcgg tgcgacgcat
     2401 ccggacgata tgaaatccgt tcgtgaagcg gcgggggata tgcctttttt ggtgccgggc
     2461 atcggagcgc agggcggcga cctggcgagc gtcgtccaat ccaacgcatc gggggagacg
     2521 ctgcgtgcat tgatcaattc gtcccgtaat attatttatg cttccaacgg aaaagatttt
     2581 gaagacgcgg cacgtaaggc gactgaatat ttacgtgatc agataggaaa actcttgcaa
     2641 tcgggagttt gaggtattat attcgccccg cgttttttga agtacatact tcgggcgctt
     2701 agtcacgctg ttggcgtgac gtcttgttgc cccacgtttt tcgggcgctt agctcagctg
     2761 gtttagagcg tctcgcttac accgagaagg tccttggttc gaatccatga gcgcccacga
     2821 taaaaccttc gattataatc ggaggttttt gtttttaacg atatagatgc tgactattga
     2881 tagattgcgt cagagtcccg atttgaaaag agtttttgag tatagagatt tgtgaataac
     2941 ccttgttgca ttcactgttg aagatcacgc aacttaacaa agaattaaaa taattaacat
     3001 caatttttat acttcagcta ttttgtagct gaaattcaat tttaagaaag aaatgatctc
     3061 atgtttcatg ttcgcttcag caaagtgata gttattgttt tgtgtgtggt tcttaataca
     3121 ggttgttcga gttcgctttc catcaatttg gagaactcat caaatcgaga ggcgcttaat
     3181 tctaaaataa atgatcgtac atgtgaaata aacatcaaaa aaaggggaac gataaaggcc
     3241 gaccaagttg cgcttacgga ttcacacatt atttggtccc atgtgaaaac cgcacaaaga
     3301 gatagcgcgg cattaaaaga tttgatcatg ctttcatggc gcaatcgtaa ggaaggtatg
     3361 tatcgcggct cggcgatcgg atttgcaaca ggagcggttg gaagcgtagc cgtgacaaaa
     3421 cttggatatt ccggtgttcc tttttctgcc ggtgattatt tcctgattgc tatttctaat
     3481 ggtttattgg gaggattggt tggaattccg gcgggattac ttttcggaaa aaaatacgaa
     3541 atatatccct gtttgccgaa ttcaaaaaat gattgcaaat aatcgtagaa ttttgaattt
     3601 aaaaagtttg tttacctatt tcattcgtct tatgtgcgca gacgcatgtt gtttcttttc
     3661 atgtaggcta cgccgtgctc atgtagcaat caggtgcaaa tttataatag agaatgggag
     3721 ttttgatgta aatagcgtta gatgatgtct gcgtctcagt cctaataatt cattcacttt
     3781 ttttttgaaa ccaattcccc ttcgaagttt tcctcttgaa tttcttcgcc cggtcagata
     3841 aactacctgt accaatctcc gtctacctaa gtatttatac gttctcataa agttttacac
     3901 tgcgaggttc catgccttct gaaaagtcca agtcgcaagg aaaaaaaacg tcttcgacca
     3961 cgcccaaaac gatgattttc ggcgtagaag atgccaaaaa actgcgcgaa gccattgccg
     4021 ccaatccgac cgtcaatcct tcacccaatg aagcggatgc caaaacggcg gagtccaaag
     4081 aaaccgccga acacaagcgg atgaaagaat ttacgcccgg cgttccgccg tggcgccgat
     4141 ggggcccttt tgtgtcggag cgctcctggg gttcggtgcg cgaagactac agcaaagacg
     4201 gcaatgcatg ggcgtatttt tcgcatgacc atgcacgcag caaagccttt cggtggggcg
     4261 aagacggcat cgccggatgg tgtgaccgct atcaggtcgt gacgtttgct ccggcatttt
     4321 ggaacggcaa agatccgatt ttgaaagaac gcttgtttgg cctcacgccg ctggaaggca
     4381 atcacggcga agacgtgaaa gaatattatt tttatctcga cagcgtaccg tcgcacgcgt
     4441 ataataaata tctttataaa tacccgcagg cggaatatcc ctatcagtgg ctgatcgacg
     4501 aaaacaaacg ccgtcagggg caaggggaag aatttgaact tttggatacg ggtctttttg
     4561 atgaagatcg gtactttgat gtttttatcg aatacgccaa agccgggccg gaggatatac
     4621 ttattcgcgt agaagtgcat aaccgcggcg atcaggatgc accgtttcac tacgtgccca
     4681 actattggtt tcgcaaccgc tggggctgga gtaaaccgcg tacgggcgaa ccgaccatcg
     4741 taccgggtcc gaaaggaaaa aacttcgtca gcgtcatcag cgacgatacg acgatggaga
     4801 cgtttaaaaa ccttcctttt ctgtatcaac tcggcaaacg ctatctctac gccgagggca
     4861 atccggaaat ttattttacg gataacgaat cccatcacga aaaactctac ggcgtaccaa
     4921 gcgccaagcc gcacgtcaaa gatgcttttc accgctatat cgttaacaaa gaaaattgca
     4981 tcaacccgga tcaggtcgga acgaaatcgt gttttcacta cacggatacg ataccggcca
     5041 aaagcagtaa agtgtatcgt ttgcggctga cggcggagac gcttcagtcg ccgtttgaaa
     5101 aatttgatga aaaagtccgc gaacgtcaaa acgaagcgga cgaattttat gaaaccgtgt
     5161 atccgcccaa acttagcgcc gatgaacgca acgtcatgcg tcaggcctac gcaagtcttt
     5221 tgtggaccaa acaaatctac ctgtacgatg tcaatcgctg gctgacgggc gatgatccta
     5281 cgatgccgcc ctccgaatcg cgctggacaa tacgcaatca ccactggcgc catctgaact
     5341 cgatgcgcat tatctccatg cccgacaaat gggaatatcc gtggtttgcc gcgtgggatc
     5401 tggcgtttca cacgatcgct tttgccgatc tggatatcga gtactccaaa gaaatgctgt
     5461 ggatcatgct tttcgagcaa tttcagcatc ccaacgggca aatcccggct tacgaatggg
     5521 aattttccga tctcaatccg ccggtgcacg ccatggccgt gtggaaagtc tatacgatgg
     5581 atcgcgatcg taccggcaaa cccgatctgg tatttttaga aaaatgtttt cacaaactgc
     5641 tgatgaactt tgcctggtgg atcaataaag tggacgcgtc gggcaataac gtgtttgaag
     5701 gcggatttct cggactggat aatatcaccg tcgtggatcg cagcgaaaaa ctgcccggcg
     5761 gtgcagtact ggagcagtcc gatgcgaccg gatggatggg tatgttttgc ctcaatctta
     5821 tgcgcatctg tctcgaactg gcgcgctaca ataaagtcta tgaaggcctt gccacaaaat
     5881 ttgtccaaca ctacatctac gtcggcgccg cgatgaaaaa catgggcgag caaggcatcg
     5941 aactgtggga cgaacaggac ggattttttt atgacgtatt ggtatatccc aaccacagcg
     6001 gttacgaaaa atttcgcgtg cgttctttgg tcggtctcat tgccatgtac gcgatagaca
     6061 ttctcaatgc caaagatgtc gaagcgaata aagaatttgt ttccaatctg gattggtttg
     6121 tacgcaatcg cccggacatc gtccagcgct gcgtgtatat tgacgaaacg gatgcttcca
     6181 atcggcgtca cattttgtcc atcgtggatg cgcatcagct ccagcgcatc atggagcgta
     6241 tgtgcgatcc ggaggaattt ttatcaccgg caggactgcg cagtttgtcc aaataccatg
     6301 cggagcaccc gtattatttc ggtgagaaga aagtggaata cgaaccggcc gaggcgacca
     6361 gcaaaatcaa aggcgggaat tccaattggc gcggcccgat ctggtttccg acgagctacc
     6421 tgatgatcaa cgctttgcag aaattttccg aagcgtacgg cgatgatttc ggaaccacac
     6481 gcgacggcaa attgattcgc cccaaagatc tggcacaaga catcgcggag cggatgattc
     6541 gcatttttac acgcgacgcc aaggggcatc gtcctgtcta tggcgacatt aagaagtttc
     6601 aaaacgatcc gtattggaaa gattatgtat tattcttcga atattttcac ggcgataccg
     6661 ggcgcgggct gggcgcttcc catcagacgg gatggacagg cctgatcgcg acgttgctgc
     6721 aggaatggag acgataaatg aatgcattgt ggtctctggt gttacgcttg tgcttttatt
     6781 tcacatgcgt cgcttcattt ttatcggccc agactccaac ttccggcgct ctcaacatat
     6841 tcgttcaaac cggtcattcg agccaagtac tcgggcttga caaaacatcc gatgatcgtt
     6901 ttttggtgac gggaagcacc gatgccaccg tcaaactttg ggaaatcgcg tccgccaagg
     6961 aggtgcgaac gttgcgagga cacaccgcgc ccgtgacgca tgtggccatt tctccggacg
     7021 ggaaaaccat tgtttcggct tcgtcgaaag attcgagtgt catgatctgg gagagtgttt
     7081 cgggtgttcg aaaattgaaa atacggttga attacgaaat gaccgccatg ctggtgcggc
     7141 ccacatggaa ttcgattttg gtatcgtcat cggaggatgc ggtcatcgaa tacagtttac
     7201 aaacaggaaa agaagaacga cgttttcaaa atgtcggttc tctcctatgt atatcggatg
     7261 acggtaaaaa agccgtctcc cgcctctatg accgacccga tggtgtgatc gtcgcatcgg
     7321 cagagcgatt gcacatatgg gatttactga acgggcaaac catccagaag ttggattcgt
     7381 caaacgccat gacggccgcc attacgcgcg atacgcgtta tgtcgtaacg ggcgaaagga
     7441 acaatacgat caaactttgg gacatcatgg cggggcgaag tgtgcgcaca ttcggttatg
     7501 aaaccgaacc ggctatgttt gccagtgatg aaatggatga ggttatttcg atttttttct
     7561 ctccggatga aaagaaaatt ctatccgctc ataaattatc agggtattat gtatgggata
     7621 gaaagaccgg cgaacccttg cgccaactgc acgcggattc gtatcgtaag gtcgatgaaa
     7681 tgaaggctga gaatggggtt gtcagtatca gggcctgcgc atttatgaag gacggctcta
     7741 caatttttgg tatagacggc cgtcgtatcc atatgtggaa caatgatacg acggaaccta
     7801 tcctgcgtta tcaaggtgtc gtgcaaggtc gaataaatga cgtacgcaca accccggacg
     7861 gcggatctct catcctagca aattccgaaa acgggctccg tttttttgat gttcactccg
     7921 gaaaacctgc ggcggccttt ctttcgaccg tcagtgacat acaaagtgtt gcgatttcaa
     7981 acaatgggaa gtttgttttg ttggtggatc aggcatctgt ggcatcactg tgggacatca
     8041 cggccaaaca acctttgtgt accttcaaaa cggaagtccc atttgctgca ttgtccgatg
     8101 atggcgaaat agctatttcg gccaatgaaa atgaatttgc agcttaccgg acccgcgacg
     8161 gtgtgatgtt gagtacatgg cgatcgcctc acatcacttg gagtgacata acgttcattc
     8221 cgaattcgcg gcgggtggcc atcgggtttt ggaaaacgcc ttatgcgccg ttcgacgggt
     8281 tattaaccgt gtgggatata ccaagcgggc aattggtatt tgagttgcac agtgaagcgg
     8341 gaacaccgcg gtgcgtctcc gtcagcttcg atggaaaaat gattctttcg ggtcatgaag
     8401 ataataccct gaaactatgg gacagtcatt cgggaaaact gattcgcagt tttaatacgt
     8461 tgatcccgct ggctttcatg aaaccgtatg agggaattac ggcggcaggt ctttcacccg
     8521 acggaaggtg gactgtgtcc ggaagtgcgg acggaacaat caagatttgg cgtaatgata
     8581 acggaacgga atggcgcaca ttaaaaggac ataccggcac gataggattg gtatgttttt
     8641 ctccggacag caaaagcgca tttacggcag gcgcggatgg tagtgttcgc atgtgggaca
     8701 tcgaatccgg gacggagtat gtttcattcg tttcgctcgg tgatgcggat tgggtcacca
     8761 tgacacgcga aggttacttt gatgccggcg gtgcaggcgc tcgtaatatc aatgtttcgg
     8821 ccggcgatca ggtgtacacg attgataatt tttttgaaac gtattttcgg ccggatattg
     8881 tacaagcgaa gttagcgggt cgtgctattg aaacaatggg tagctcggac ggcctgcttc
     8941 aaggcgttaa aataccgccg acgcttaaac tactggcatt agaccataac ggtcagtatg
     9001 ctccgctggt ttccgaaaac gaaaaaacgg cgaatatcag caacggggtc atccgaataa
     9061 aaattatcgc cgaagatgcc ggcggcggtg tatcgggtat ccgactgttc aacaacggca
     9121 aggcggtcgg agaaaatatt cgcggtttta aagcgacgcc cagcatcgaa aaaattgagc
     9181 aagaattttt cgttaccttg tccgaagggg aaaatatatt ggcggctgtc ggtttttcga
     9241 acgataaatc cgaatccaat cccgtcaaag cgcgcctctt ctaccgcaga cctgttcttg
     9301 caaaaccgga tatgcatatt ttggctatcg gtatcaatca gtatcgtaat gccaaatata
     9361 atctgaatta ctgtgtcggt gatgcgcaag gttttgtcaa tgcgattacg cccaaagcga
     9421 aaaaaatctt ttctaatata tataccacgc ttttacttga tcgagaagcc aaccgggccg
     9481 gtgttttggc ggcattggca tccatacaag ccgctgcgaa accggaagac gtgttgatta
     9541 ttttttatgc aggtcatggc atggccatgg atatcgaaga aaacggccca tcgcggaatg
     9601 agttttttta tatcctcagc gaagttacgc aaatgtcgga tccgatgaag tgtgcgcagg
     9661 aaggtatttc cgggaccgaa atgcgtaaaa ttttgtctga tatcaaagcg accaaacaag
     9721 tcatgttcgt ggatgcctgc aattcaggtg cgtttgccca gcagtttgcc gtgcgcggtg
     9781 cagcggagga aaatgcttta gccaaactga gccgggcttc ggggtcggtg atctacgcat
     9841 ccaccacgaa ggaacaattt gccacggagt tagccgcact caaacacggc gcttttacct
     9901 atgttttgat tgatgcgctg ggcggtcaag ccggtttacc caatggtcaa ttgaccgtct
     9961 caagtatcaa agcgtatgtg gatgataaaa ttccggaggt cacaaaaaag tataagggtg
    10021 aagagcagta tccgacgatt tttatttggg gtcaggattt tccgatagga atgaagtaat
    10081 caggatcgat acgtgtcgca tacaaaacaa aaaacccgct ttgtgagcgg gtttttttat
    10141 cggatgcaaa tcgcgattaa gcaacgtttt tggaaacgag cttggcgaga tcaaacatgg
    10201 agacttggct cttgccgccg aaaagggcgc ggagtttgtc gtctgcattg atcatgcgtt
    10261 tgttcgtttt gtcctggaga ccattcttct tgatgtagtc ccagatcttt ttgatcattt
    10321 ccgtacgggg acgggcttga tcgccgataa cagcggctaa agtcgcgctg ggcgtgagtt
    10381 tagccataaa cgccgcattg ggtttacggg ctgttttttt cttcgccggt ttggctgctt
    10441 tcttcggttt agcagctttt tttacggtct tcttcgcagc ttttttagct ggttttttct
    10501 tggcaggctt ctttgttgcc atggtggtac ttccgttgtt tagttagtga gagtaagtta
    10561 aatgacccta tattatctcg taaaaaaagg aatttttcaa agtaaaaatt aaaaaaatta
    10621 aaaaaacttg cccgttgccc attatgaaaa ataattgcca aaatgataat taatggataa
    10681 catcgcttca tttcttcgtg ggatgtgaag ggcatcgtct aaataattta taaaaaaatg
    10741 cacgtaattt ttttatacca aacttaagga aaaaacatga aatcagtatt tgatcctgca
    10801 gcgcgtcagg aaatcattga tcgtgtaaat aagctctcgc ccgcgacaca gccccaatgg
    10861 ggtaaaatga atgtggcgca aatgctggcg cattgcggca ttagttttcg tgtccccacc
    10921 ggcgacttta cgatccaaaa gacatgggtc agatttatcg gccgtttttt taaatcgctg
    10981 gcgacaaacg ataaaccgtt tcaaaaaaat tcacctaccg cagcggaatt tttgatcacc
    11041 gatgaacgaa tttttgaaac tgaaaaaaag aattttattg ataactttca aaaagttagt
    11101 cgcggtccgg aagcgatcac ttatttcgaa cacggctttt tcggttatct gactgcggag
    11161 gaatggggca aaatgatcta caaacatacc gatcatcatc ttcggcagtt tggcgtataa
    11221 tatgattaaa aatttttgta tagcttgctg gcttttttca tccgtcgttt tcgcgcagta
    11281 caatgacagt gaattatacg tcaccgtttt atcgcaacat gttcactccg gaaaagtgga
    11341 ttatgcacga ttacgcttgg atcgtcgatt ttccaattat cttttcagcc tgacggaagt
    11401 gaatcccgat accattcaaa atttgaacgc caggaaagca ttttggatca acgtatataa
    11461 cgcctatacg ctgagcgtgg tgcgcgacca ttatccggtc aaaagtattc gcgcaattga
    11521 caccggatta ttcggtacga gtgtttggga taaaccgtat gtccatgtag gcggaaagaa
    11581 atattctctg aatgatgtcg aacataaaat cttgcgaccg atgaatgatc cgcgcattca
    11641 ctttgccctg gtatgtgcgg ctaaaagttg tccggttctg cgcaatgaac cgtatcgtgc
    11701 cgaaaaattg gatgaacagt tgaacgaagc cgctcgtcaa tttatgaacg acccggaacg
    11761 caacagggta gatcatcaag ccaaggcctt atacttgtcg tccattttca aatggtacgg
    11821 aaccgatttc ggcaaaagta ctggggatat ggtcaggtat cttttgcggt atatggatat
    11881 aaacgatacg gatcgcgtgg tgtcttatcg tgtagaatat ctcgactacg actggtcact
    11941 caacgaataa gtggccgcat ccggcggcaa ggtacggcta tttgatttct attatcattc
    12001 ctacgtataa cgaaatccaa aacatcccta ttcggatcga agaatgcagt gcattgacgg
    12061 atgcacaaat catccttgct gacggcggta gtaccgatgg cacggcggaa tacgttcgtg
    12121 atgccgccat acggaataac cacttagatt ggattcaatc cggtaaaggg cgtgccctac
    12181 agatgaatgc cggagcccgt ttggcttcag gggcatggtt cgttttttta catgcggata
    12241 cttctttatc tcaaagcagt tgggaggtat tacgtaaaac catcgaagaa aagccacaat
    12301 taattggcgg tgctttttat ttgcaaatgg atcatccggg gtggaaatac aattatttgg
    12361 cgtggtattc aaaacagcgg agccgtttat tcaaaacacc atttggcgat caggccgtat
    12421 ttgtgaaagc cgatatattt cgtgcattgg ggggataccg tgaagatttt ccgatcatgg
    12481 aggatttgga ttttgtccgg cgattaaaag cagccggaga ttttgaaatg ctgccggctt
    12541 atgtggtgac gtcggcgcgt cgctttgcgg aggaaggttt tttcaggcgc agttgtaaaa
    12601 atcttttcat gcagacgttg tattgttttg gcgtttcgcc gtcgaggtta aaacgatggt
    12661 atgaatagaa cataatttga acgaaattga aaaaacgagt ataactaatt taagaactaa
    12721 agttcatttt tcggtcaaat accttagcat ggccaggtta tcagaaaaca catcatttta
    12781 cgacttaata atataatatt tcatattctc ccgatccttc attcagatca aacctttcgt
    12841 ccgcttttta actcatatca gtcaataaag ttcactgtgt ttcgttatcc ggaaagcgta
    12901 acattattta ttttcaaatt tccaaaaaaa atggaactaa gtggaacttt cgggcgttta
    12961 atgaaagcaa ccaagaatta aagtatctgt aaaatataaa cttaacacga aagagaatgt
    13021 ataatggccc gcattaaaat gcgcattaac agccgggaag atcagtctat cgagcgttat
    13081 ctggaagaga taaacaaagt acaactttta aagccgcatg aagaaatcga acttgccaag
    13141 cggatcaaaa agggagaaaa ggaagcgctc aaaaaattgg tggaagccaa tttacgtttc
    13201 gtggtgagtg tggccaagga ttatcagaat caaggtttgc ctttgagcga tctgatcaat
    13261 gaaggtaatc tcggccttat gaaagcagcg gaacgtttcg acgaaacgcg cgggttcaaa
    13321 tttatttcgt acgccgtgtg gtggattcgt cagtctattt tgcaggctat tgccgagcac
    13381 tcccgtatcg tgcgtctgcc gctcaaccgt gtcggcatgc tcaataaaat cggtaaagcg
    13441 tataacgaac tcgaacagga atttgagcgt gaaccgagta cggaagaaat cgcgcacgaa
    13501 ctcgaaatca aagattatga cgtatccgat gcgttgcgta tttccaagcg ccacttgtcc
    13561 ttggatgcgc cgctgcagga agacgaaaaa agcgtactga tggataccgt gccggataaa
    13621 gtcaatccgc ttccggacga aacacttatg caggaatcgt tgcgccgcga tatcgaaaac
    13681 acgctggctt cgctgagtga gcgcgaatcc aaaattatca aattatattt cggtctcggc
    13741 atcgagcgtt cgctgacact cgaagagatc ggggaagaat ttaaactgac ccgcgaacgc
    13801 gtacggcaga tcaaggaaaa agccatcaat cgcctgcgcc acaaatcccg cagtaagtcg
    13861 ctgcgttcat atctgggtta atgttgtctc gggtcccgat gctaagcgtc gggacttctt
    13921 ttttgatcga aagtattaac tatggttcag gaaacactcc acccgcaaaa agtttttttt
    13981 gagcgcggac gccagttcgt cgaagattgt gaagaacagt ttctcgcgcc gtacgcctgt
    14041 aaaagcaaag atgcgcataa aacgcgccag catggtgaaa tggaagacgt gcaccgcacg
    14101 gcgtttcagc gcgaccgcga tcgtatcatt tattccaatt cgtttcgcaa tctgtccgac
    14161 aaacgccaaa ttttggtcga gcacgacagc gaacgaaacc gcagccgtct gacgcatacc
    14221 gtagaagtcg tacaagtttc caaaactatc gctaaatcaa tgggtctcaa cgaagatctt
    14281 accgaagcca tcgcgctcgg ccatgatctc ggccatccgc cgttcgggca taccggcgaa
    14341 gcgattttag atgcgctgct caacggcgaa gataccgccg aaggtacact tccgggcgac
    14401 gatttcggcg gatttaagca caactatcaa agtttgcgcg tggcggatat tctggaaaaa
    14461 aaatacgaat acgaaggact taatctgact tcttttgtac gcgaaggcat tctcaagcat
    14521 accagtattc gctccgaacg ataccgctat cctgatattg atacggacaa actgcactgt
    14581 gatcaatttg tttcgctgac gctggaagga caggtcgccg cgatagctga cgaaatcgcc
    14641 cagcgcacat ttgatctcga agatgcattg cgtgccaact acattcgtct caaagatgtg
    14701 cgcgaattaa agctcgtgca ggatgtggac cgttcgtgca gtatcgcgca tttttatgaa
    14761 gaagatccgg atgaatacat caattttatg atcagcggat tggtgcgtgt tttggtgagc
    14821 gacgtgattc aaaacaccat tcgccgggtg gaggaattct gcggacgtaa gcagcgcacc
    14881 gatcactttg acgaatggct cgtgattttt tcgcttgata tcaaccgtaa gcagaacgaa
    14941 ctggaaacgt ttattcagaa agaaatgggc aataattttc tggtcaatcg ctacaataaa
    15001 aaatgcgaaa aagtcattcg taagttgttc aaagcgtatt acagcaatcc tttgcagatg
    15061 aatgataaat ttttgatgcg gtttacgcaa aaacaggtag acgctaagtt tgatctgcgc
    15121 aagctccccc caaaaatggt gcaggaaaaa gtcagcgagt tcaaaaagaa tccgctctat
    15181 gcgcgccaca tcgcggacta tatcgccagt atgagcgata gttttgcgat tcaggagtac
    15241 aaagctatct acattcccga agcttcggta ttataaactg ccatgcgcct tctcaaaagc
    15301 catcgcattt tttttctgtc gctgtgcatc gccatatttc tttggttata cgtgcgtctc
    15361 ggcgcgacat accagatgga gatccacgtc ccgtttcaag tgaccaatat caaagacggt
    15421 tacggcgtgg tctccgatat tccggaggcc gtcgatattc tttttgaagg tgacggcaaa
    15481 tccctgcttg gtatgcgctt tctcaatccg ccgcgttttg ttttggatat ggcacagcac
    15541 aaagataaaa gccgttttat actttcggaa catcttcacg aaatcgtgta tccctccaaa
    15601 gcgccggtgc acgcaatcgc cgtacaatgg
//
LOCUS       DAOQNQ010000045        15376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1368_length_15376_cov_5.646890, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000045 DAOQNQ010000000
VERSION     DAOQNQ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15376
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1368_length_15376_cov_5.646890"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1829
                     /locus_tag="PK754_04775"
     CDS             <1..1829
                     /locus_tag="PK754_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB08848.1"
                     /translation="TQEGQQWGGEHAPEIDPFAAGSITVIRGASGLEYGAGAIGGVIR
                     VEPSPLPEHAGIGGELSLNGFTNNRQMAGALRLEGGLANKPGFGWRAQVSGRKAGNAQ
                     TPRYVIGNSGFQEFNGAAAVGFRNHRWESQLYYSHFGTELGIYKGSHIGNTSDLIAAI
                     KRNRVPLNEDDFSFAIGNPRQVITHDLISARVHRQTDAGDRFEFQGGWQRNHRQEFDS
                     HKPLGTPAEDLKKPSFDLSLYTYTADVRWRHRPIGDFAGTIGVNVMRQRNVNQAIRLI
                     PSFHTYNAGVYWIEKWVQDDWSAEAGLRYDQRWLDASFTAQQQLKLGVNEKEDHVYRS
                     TSAAVGVNRRFAEYWSIGINAGTAWRPPGVNELYSNGVHHGTAQYEIGDPAMGVEKSY
                     STDITLHHNSEKINGDVSLFVNRMDNYIHLYPSRKPTLTIRGSFPTFRYAQTEARLTG
                     IDGQIQWHALDIVRLDAGWSIVRGRDLKKDEPLIYMPSDRLSGLMHWHFSDAGTIHEP
                     YVDIGITWYRRQSHVPEGAETFVNDADSVTVTPPPGYVTVDFEVGCEWHTSWSPITIS
                     LAINNVFNTRYANYLSRFRYYIDDPGRNIVLRVQIPFGNLEF"
     gene            1870..2424
                     /locus_tag="PK754_04780"
     CDS             1870..2424
                     /locus_tag="PK754_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08849.1"
                     /translation="MTRVFSKTALLTVTILMSLFVFSGCEKDDKETNPEELITSVVIK
                     FVDTSNAANTVTASFRDLDGEGGADPVQFDTIKLAANKTYHATIVLLNETEEPADTVS
                     NEVYDERNDHMFFFHGHDVNLVVNYRDVDDNNIPVGLLTRWITGAASSGEAHVTLKHQ
                     PGVKNGTSTPGETDIEVEFPIRIQ"
     gene            2495..2815
                     /locus_tag="PK754_04785"
     CDS             2495..2815
                     /locus_tag="PK754_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08850.1"
                     /translation="MHSLLRYSYIVFYLIVCSILPRLHTDQCGISVHTVEITVRDLGY
                     STGEGAQHSYCLAHQFASGHDHVDSLPLVIASIINENKGHSHVWFLESILPRRSARAP
                     PLSV"
     gene            2976..4196
                     /gene="zigA"
                     /locus_tag="PK754_04790"
     CDS             2976..4196
                     /gene="zigA"
                     /locus_tag="PK754_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallochaperone GTPase ZigA"
                     /protein_id="HNB08851.1"
                     /translation="MTKKLPVTVLSGFLGAGKTTLLNHILKNREGKRVAVIVNDMSEV
                     NIDASLVKNGEAALSRKEEKLIEMTNGCICCTLREDLLVEVARLAKEGRFDYLLIEST
                     GISEPLPVAETFTFEDEQGESLSRYARLDTMVTVVDGYNFFNDYCGSDSLIDRNIALG
                     EEDERSIADLLIDQIEFANVIIVNKADLIGEEARKVLFSVCQRLNPDAQIVFSKYGDV
                     PLAQVLDTGLFDFDKASSAPGWLKELRGEHTPETEEYGISSFVFRARKPFHPERFWAW
                     VHTEWPGVLRSKGYFWLATRMEWAGHWEQAGGTCNYEPAGFWWSAIDRRSWPDEAAYI
                     QDIEAQMQPPYGDRRQEIVLIGKDMDKAALSQSFEKCLLTDEEMKIGHTGWTRLVDPF
                     PEWIIQSAEESVVN"
     gene            4229..4423
                     /locus_tag="PK754_04795"
     CDS             4229..4423
                     /locus_tag="PK754_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08852.1"
                     /translation="MAKKQTFGEKTDKQKRDANWKTIMIVYSSVSPKTGAWKFNERFV
                     RMPAHEDEVAFATKWLETNF"
     gene            complement(4509..4919)
                     /locus_tag="PK754_04800"
     CDS             complement(4509..4919)
                     /locus_tag="PK754_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08853.1"
                     /translation="MKKILTFLLFTGAISVYAQEVSKQLERLDPGTATANLDNFGKTH
                     SEQITAAHQARLGELIKAGTIIIRQDTMITADKPVKIAFNWPVASGQNLKDKSVVKRD
                     PQVMTLYPAKDPSNKDIPQRWQVTYKDGTVKAIQ"
     gene            5033..6208
                     /locus_tag="PK754_04805"
     CDS             5033..6208
                     /locus_tag="PK754_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015213327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HNB08854.1"
                     /translation="MKKPIYLDHHATTPLDPRVLQKMMPYLTEHYGNAASRTHQYGWI
                     AEEAVEQARIRIATAIGASKDEIVFTSGATEANNLAIKGIAETFQRKGRHIITVSTEH
                     ASVLDTCEYLREAGFEITVLEVSEDGILDQIKILNAIRSDTILVSVMLANNETGVIQD
                     MAAIGAITREKGIILHTDAVQALGKIPVHVENLNVDLLSLSAHKFYGPKGVGALYIRK
                     RNPRIQLTALLHGGGHENGQRSGTLNVPGIVGMGEAARFSVDELQQNQKHYAGLRERL
                     WSGLKQTIKGVRLNGPDFEKDDNFSRRLPNNLNVSIPDIDGEGLIMALKDIAVSTGSA
                     CSSAVPEPSHVLLRMGISKELAKASLRFGLGSGNTSDDIDYTIKRITETVGKLIKTI"
     gene            6217..6708
                     /locus_tag="PK754_04810"
     CDS             6217..6708
                     /locus_tag="PK754_04810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNB08855.1"
                     /translation="MIIEDKKKALRFFQYGMFILTAKAQHDFQGSPYAASTVTWVSQA
                     SFEPPLIMLGLRKDSWANEAVRQTDSFALNILSEDQKSIAGKFFKNIEVNGQMINGYS
                     FDTDQSGAPILDEAQAYIECTVTDRIERGDHSIIVASIINAKVRRDFKPILLRDTGWN
                     YGG"
     gene            6698..7543
                     /locus_tag="PK754_04815"
     CDS             6698..7543
                     /locus_tag="PK754_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNB08856.1"
                     /translation="MAVKRNVVIMRTIVMLAMCFLVIVSCSKPKDSAQRDLMRLGIAY
                     NDQEFLSRIRKGDLILTNMFLQAGMNPNMRFTNHKDSVSQGESNDPTPLMIASREGHI
                     GIVRSLLRKGADPTAVADNGQTALMYGVRYAPTELVSDLIVAGADVNGRDKDGKSALT
                     FAVLLDRLDVVRLLLDRGADPNAMDRFSFTPLMQAAIKGKAPIMEALLDKGANLEVRT
                     PNGETVLMLAVNYNRMEAVRLLVNRGVDLKAKDMQGRTALSIARAKKHETIIEILTTA
                     ESKNQ"
     gene            7568..9166
                     /locus_tag="PK754_04820"
     CDS             7568..9166
                     /locus_tag="PK754_04820"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNB08857.1"
                     /translation="MHFYRMLIVILCCSLSLFAQAEKGDSVAAEAIKTEAIERSQALQ
                     TLNVLTNVIGARLTNSKGLAAANDWTREALEKMGLSAEVEPWGEFGRGWDMKSFRAGI
                     VAPYAIPLIAYPKAWSPATRGTVRSKILYINETKLEDIQKKYSNKLKNAIVLLGDKVK
                     IEEDFEADAKRWSDSELLTMSNAGKNEGRAESFRRSFRKSPRFSAFIERQRIFTWINN
                     QQPALIVDGGSFSFRGGTPHSGVVRVMGASMPLVDESDPFGNTNPKAWDPNPPSVAPQ
                     IAISAEQYNQLIELIKSGKEVIMECDLQVEWRDKDLKAYNTIGTWSGSDRKDETVMIG
                     AHLDSWHSSTGATDNAAGVSVMMEAIRILKAIGFQPRRTIKIGLWSAEEQGLLGSAGY
                     VNKHLYDSTGAKPGYEKFNVYFNMDNGGGQIRGIYLQGHEDVQSYFRQWMEPFRSWGA
                     STISLKGTGSTDHMSFISAGLNGFQFIQDPMDYSNITWHTNMDMYDHIQPLDLKRNAA
                     IVAYFVYMSAMSDEVLPKRAIEKE"
     gene            complement(9300..9905)
                     /locus_tag="PK754_04825"
     CDS             complement(9300..9905)
                     /locus_tag="PK754_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNB08858.1"
                     /translation="MKKYIKIMTVAIAVSFLVPLSVVHSQIAGLDIKLGVKGGFNYSL
                     FGGKDNDQTKGKTGYNVGVFALYELMPMIAVQPELLYTLKGARTTTPGQNFEYYYQYF
                     EMPVLMRVGMDIEEQKFYFIGGPSFSYAKKAYTRDREGSATVLTKPKKSDYGIIAGLG
                     TGTADSKITVEGRIYFGVPDVVKKTKVNNITYSIGMGYLIY"
     gene            complement(9938..13264)
                     /locus_tag="PK754_04830"
     CDS             complement(9938..13264)
                     /locus_tag="PK754_04830"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNB08859.1"
                     /translation="MRGSFYSRNWRGTFVYAAMFLFALTLNLRAQVTTSAISGMVKDN
                     NGTPLPGVNIIAVHEPSGTKAGAYSRSDGRFNLPGMRVGGPYTVTASMIGYKEHKIEN
                     INLALGEEKNLTFVLVEEAVEMEGSEIVAERNPIINDARTGAATTVNELQVKYLPAVT
                     RDFNDFTKYTPQFSGNSAAGRNNRYNNISIDGAVSNDLFGLAASGTPGGQAGTAPISL
                     DAIQEFQVQIAPYDVRLGNFTGGNVNAITRSGTNKFEFSSYYNYRNENFQGESFDGVL
                     PTKNFIRTEGARLGGPIIKDKMFFFVNYDHELQEVPTNYGILGDGSSLDFPGTGSRLS
                     VKKARDIQNFLKDTVLHDPGSFGMSTIPTESYKFFARLDFNLTPEHRFTIRHNYINAN
                     QDLFRRETGTNSSSSSGGFRFSNSRYIFKNITNSTVAQLNSTLSKEMFNELTMSYQVI
                     DDHRETPGDRFPYVMIRDSVAGLSYVIGTEQFSQFNKLKQSVFEFTDNFTYYMGEHTF
                     TIGTHNEYFKFENGFLPSFNGRYDFNSFENFKRSYYGLTGNIREYNLTFALDPNDPTG
                     FNADTTDRVPLAKFSVMQLGGYIQDAFKVTPSLTITAGLRFDVPIISDKPTANDSFAI
                     NYARFYHTGGENDTTIKNAKYRTDKIASGNILWSPRIGWNWDVNGDQSLQFRGGFGIF
                     SGRNPYVWISNQYSNTGADLGTIRLTGGSTLTNMTFQKDPTKQPTAGTLGSSTINVMN
                     PKFKNPQVFRVNLALDQKLSDTWVGTVEFVGSDNINDVMFKDISLAGPQSYSPTDNRP
                     RFGTISTYTSPSSSATTSPARVLPLSKSKQYTNVIYLTNTKWGYQWNASVQLQKLYDN
                     GYFGSVAYTYGRATDMTSATSSIALSNFQFNPVKGDPNNVKTATSNYEQRHRIIVSVA
                     KEIEVAPKWKTVFSLFYSGFSGRPYSTTYNGDVNADGNTGNDLIYVPKDKNDIVLVPT
                     SNSDTRSADQIWVELDEFISGDPALRKARGKIVDRNASTEPWTNRVDFQITQELPVLK
                     ELGKFELTANVINVMNIFDNEWGQSKSIANQNDQAIQFRGIQSGTGKPVFSFTKRPDR
                     YSISTTASRWAVLVGARYSF"
     gene            13506..13579
                     /locus_tag="PK754_04835"
     tRNA            13506..13579
                     /locus_tag="PK754_04835"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13540..13542,aa:Arg,seq:ccg)
     gene            13762..15204
                     /gene="proS"
                     /locus_tag="PK754_04840"
     CDS             13762..15204
                     /gene="proS"
                     /locus_tag="PK754_04840"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNB08860.1"
                     /translation="MAERITKREQDYSQWYLDVIAQAQLADYAPVKGCMVIRPNGYAI
                     WELVQQSLDKMFKDTGHVNAYFPLFIPESFMTKEKEHVEGFSPECAVVTHGGGKKLEE
                     SLYVRPTSETIIWSMYKNWIHSYRDLPILINQWANVVRWEMRTRLFLRTTEFLWQEGH
                     TAHATYDDAEKEAMQMLHVYKTFAEEYMALPVYHGKKTDSEKFAGALHTYCIEAMMQD
                     KKALQAGTSHNLGQNFAKAFDVKFQDENAQLQHVWATSWGVSTRLIGALIMAHSDDNG
                     LVLPPKLAPMQVVIVPIYKEDHERTATVEAAQKLKSDLQSKGIRTHVDDRDQYRPGFK
                     FNEWEVKGVPIRVELGPKDLAAGQIVCARRDKTAKTDKITMAVNEASSRIPALLEEIQ
                     KNLFDRAKEFRDKNTIFVSDYAGLKKIVENEEGFARCFWAGSREDEAKIKEETKATIR
                     CIPMEQSGEKGRCVLTGKETNVQVIFAKAY"
BASE COUNT         4262 a         3193 c         3700 g         4221 t
ORIGIN      
        1 taacgcagga aggtcagcag tggggcgggg aacacgcgcc cgaaatagat ccgtttgcgg
       61 ccggtagcat cacggtgata cgcggcgctt ccggtttaga atacggagca ggcgctatcg
      121 gcggtgtgat acgtgtagag ccaagcccgt taccggaaca tgccggaatc ggtggcgagc
      181 tttctctcaa cggttttacg aataaccgcc aaatggccgg agcattgcga ctcgaaggcg
      241 gccttgccaa taaacccggt ttcggttggc gtgcacaggt cagcgggcgt aaggcgggta
      301 atgctcaaac accgcgttat gtcatcggca attccggatt tcaggagttc aacggggctg
      361 ccgcggtcgg atttcgaaac catcgctggg aatcgcagtt gtattatagt cattttggaa
      421 cggaacttgg tatctacaaa ggttcgcata tcggaaatac cagcgatctt atcgctgcga
      481 tcaaacgcaa ccgcgttcca ttaaacgagg atgatttttc cttcgcgatc ggaaatcccc
      541 gtcaggtaat cactcatgat ctgatatcgg ctcgtgtaca ccgtcaaacg gatgcgggcg
      601 atcgtttcga atttcagggt ggctggcaac gcaatcatcg tcaggagttt gattcgcaca
      661 agccactcgg tacaccggcc gaggatttaa aaaaaccttc ctttgatctt tcgctgtaca
      721 cctacacagc cgatgtgcga tggcgtcatc gtccgatcgg ggactttgcc ggaaccatcg
      781 gcgtcaatgt catgcggcag cgtaatgtca atcaagcgat acgtctgatt cccagttttc
      841 acacttataa tgccggtgtg tattggatcg aaaaatgggt gcaggatgat tggtcagccg
      901 aggcggggtt gcgctacgat cagcgatggc tggatgcttc ctttacagcg caacaacagt
      961 tgaagttggg tgtaaatgaa aaagaagatc atgtttatcg cagtacatcg gcggccgtcg
     1021 gcgtgaaccg acgttttgca gaatactggt caatcgggat caatgccgga acggcgtggc
     1081 gaccgccggg agtaaatgag ttatatagta acggtgttca tcatggcacg gcacaatacg
     1141 aaatcggcga tcccgcgatg ggcgtagaaa aaagctatag tacggatatc actttgcatc
     1201 ataacagcga aaagatcaac ggcgatgtga gtttgtttgt aaaccgcatg gataactata
     1261 ttcatttgta tccatcgcgt aaacctacgc tcacgatcag gggatctttt ccgacgtttc
     1321 gttatgcgca aacggaagct cgattgaccg gtatagacgg ccagatccaa tggcatgccc
     1381 ttgacatcgt gcgtcttgat gccggatggt ctatcgtgcg cggccgcgat ttgaaaaaag
     1441 acgagccctt gatttatatg ccttccgacc gattaagtgg gcttatgcat tggcattttt
     1501 cagatgcagg cacgatccat gaaccgtacg tggatatcgg gataacatgg tatcgtcgcc
     1561 aatcgcatgt acctgaaggc gctgagacct ttgtaaacga tgccgactcc gttacggtca
     1621 ctccgccacc cggttatgtg acggttgatt ttgaagtagg gtgcgaatgg catacatcct
     1681 ggtcgccgat tacgatcagt ctggccatca ataatgtgtt caatacgcgg tatgcgaatt
     1741 atctgagccg gtttcgatat tatatagacg atcccggtcg caatattgtt ctgcgtgttc
     1801 agattccgtt tggcaaccta gaattttagt taaaattaat cattaatcga aaaacttaag
     1861 gagtgttgta tgacacgagt tttctcaaaa acggcgttat taactgtaac tatactgatg
     1921 tcattatttg tattcagcgg atgcgaaaaa gacgataagg aaaccaatcc ggaagaactc
     1981 atcacatcgg tggtgattaa gtttgtagat acgtcgaatg cggcgaatac ggttaccgcc
     2041 tcgtttcgcg atttggacgg tgaaggcggc gcggatccgg tacagtttga tacaattaaa
     2101 ttagccgcaa acaaaaccta tcacgctacg atcgttttat taaacgaaac ggaagaaccc
     2161 gccgatacgg tttcgaatga agtgtatgat gaacgtaatg atcatatgtt tttctttcat
     2221 ggccacgatg ttaaccttgt tgtaaactat cgcgatgtgg atgataataa tattcccgtc
     2281 ggtttattga cgcgttggat taccggtgcg gcctcaagtg gcgaagcgca tgtgacgctt
     2341 aaacaccagc ccggagtcaa aaacggtaca tctacacccg gcgaaacgga tattgaggtg
     2401 gagtttccga ttcgtattca ataaggtttc ttataaatac aaggcgttac ttaccatgtg
     2461 acgcctttat tagttctcat cggaaagcgg aaacgtgcac tctttgttac gatattcata
     2521 cattgttttt tacctgatcg tttgcagcat tctaccgagg ctgcatacgg atcaatgcgg
     2581 catatcggtt catacggttg aaattaccgt ccgcgatctc ggttattcta caggggaagg
     2641 cgcccaacat tcgtattgtt tggctcatca atttgcatcg gggcacgatc atgttgattc
     2701 gttgccactc gttattgcgt ctattatcaa tgagaacaaa ggccacagtc atgtttggtt
     2761 tcttgaaagt attttacctc gtcgttctgc tcgagctccg ccattgtccg tctaaataca
     2821 cattcacatt tattccttaa ataacgaaac ctctaacgga ggttttccat tattcctttg
     2881 tcattggcct tacgcaaaag atcggtatta tgtattgccg aactccggcc tgtgatgatt
     2941 tgtacttcat ttacacttat tttagggatg cttttatgac taaaaaactt cctgtcaccg
     3001 tgctttccgg ttttttaggc gccggaaaaa cgacattact caatcacatt ttgaaaaacc
     3061 gcgaaggtaa acgtgttgcg gtaatcgtca atgatatgtc cgaagtaaat attgatgcat
     3121 cgctcgtcaa aaatggcgaa gcggccttaa gtcgcaaaga agaaaaattg attgaaatga
     3181 ccaacggatg catttgttgt acattgcgcg aagaccttct ggtcgaagta gcgcgactgg
     3241 ccaaagaagg acgatttgac tatctcttga tcgaatcaac cggcatatcc gaaccgttgc
     3301 cggttgccga aacatttacg tttgaagatg aacagggcga aagtttatcg cgctacgcca
     3361 gattagatac gatggttacc gttgtggacg ggtataattt ttttaatgac tattgcggca
     3421 gcgattcact gatagatcga aacattgctt tgggtgagga agatgagcgt tcgatcgccg
     3481 atttgttgat agaccaaatt gagtttgcca atgtcataat cgtaaacaaa gcagatctga
     3541 tcggtgaaga ggccagaaaa gtgttgtttt ccgtatgcca aagattgaat cctgacgcgc
     3601 agattgtttt ttctaaatac ggcgatgtgc cattggcgca agtattggat acgggacttt
     3661 ttgattttga taaagcttcg tcggctcccg gttggcttaa ggaattgcgc ggtgaacata
     3721 cgccggagac ggaggaatac ggcatttcca gttttgtttt tcgtgcgaga aagccatttc
     3781 atcccgaacg attttgggcg tgggtgcata cagagtggcc cggagtgctt cgatccaagg
     3841 gttatttttg gctggcgaca cgcatggagt gggcggggca ctgggaacaa gctggcggca
     3901 catgcaacta tgaaccggcc ggtttttggt ggtcagcgat agatcgccgt tcatggccgg
     3961 acgaagcggc gtatatacaa gatatcgaag cacaaatgca accgccatac ggagatcgcc
     4021 gtcaggaaat cgtcctcata ggtaaagaca tggataaagc ggcgttaagc caatcatttg
     4081 aaaagtgttt gcttaccgat gaagagatga aaatcggcca taccggttgg acgagattag
     4141 ttgatccgtt tcccgagtgg ataatccaat ccgcggaaga gagcgtagtg aactaatcta
     4201 tgaactttta tatggagtta taaaaaatat ggccaaaaaa caaacatttg gcgaaaaaac
     4261 ggataaacaa aagcgcgacg cgaattggaa aacgataatg attgtttatt cgtcagtttc
     4321 acccaaaaca ggggcatgga aattcaatga acgatttgtc cgtatgcccg ctcatgagga
     4381 tgaagtcgct tttgcaacaa aatggcttga gacaaatttc taattcaaaa tatcctcagt
     4441 taggtccact aaaaacccct tcatccgttt tgatgaaggg gtacttattt gataactaaa
     4501 caaaaaattt attgtattgc tttgactgtg ccgtctttgt aagtgacttg ccatcgttgt
     4561 ggaatatctt tgttgctcgg atctttagcg ggatagagtg tcattacctg cgggtcacgt
     4621 ttgaccacgg atttatcttt gaggttttga ccgcttgcaa ccggccagtt aaatgcgatt
     4681 ttaaccggtt tatccgcggt gatcatagtg tcctggcgaa taatgatcgt accggccttg
     4741 atgagttcgc ccagacgagc ttgatgtgct gccgtgattt gttcactgtg agttttaccg
     4801 aagttatcta ggttggccgt ggccgtaccc ggatctaggc gctcgagttg tttggaaact
     4861 tcctgcgcgt aaacggaaat tgcgcccgtg aacaataaaa acgtcagaat ttttttcata
     4921 ttaatttcct tttggtttag cctatcttac gaaagctaat tttttttaac aagttttttt
     4981 acatcggtat gtcggttgat tttatgatag tgattatcta ctttgcgtcg ccatgaaaaa
     5041 accgatctat cttgatcatc acgcgacgac gccgctcgat ccacgcgttt tgcaaaaaat
     5101 gatgccgtat ttgaccgagc attacggcaa tgccgccagt cgaacgcatc aatacggttg
     5161 gatcgccgaa gaagcggtgg aacaagcgcg aatccgcatc gctacggcta tcggggcaag
     5221 caaggatgaa atagtattta cgagtggtgc gacggaagct aataatcttg caatcaaagg
     5281 catagccgaa acgtttcaac gcaaagggcg ccatatcatc acggtttcta ccgaacacgc
     5341 cagtgtatta gatacgtgtg aatatttacg agaagccggt tttgaaatca ccgtattgga
     5401 ggtttcagaa gacggtatat tggatcaaat caaaattttg aacgccatac gaagcgatac
     5461 gattttggtg agtgtcatgc tggccaataa tgaaaccggc gtgattcagg atatggctgc
     5521 tattggagct attacacgtg agaaaggtat tattcttcat accgatgcgg tacaagctct
     5581 tggcaaaatc ccggtgcatg tagaaaacct taatgttgat ttattatccc tttcagcgca
     5641 caagttttat ggtcccaaag gtgttggcgc actgtatatt cgaaaacgta atccccgtat
     5701 tcaactcacg gcccttttac acgggggagg tcatgaaaac gggcagcgtt ccggtacact
     5761 taatgtgccg ggtatcgtgg gcatgggcga agcggcgcgt ttttccgttg atgaattaca
     5821 acaaaaccaa aaacactatg caggacttag ggaacgccta tggagcggct taaaacaaac
     5881 aataaaaggc gttcggttaa atggtcctga ttttgaaaaa gatgacaatt tttcccgtcg
     5941 gcttccgaat aatctcaatg tcagcattcc ggacattgat ggcgagggac ttatcatggc
     6001 gctcaaagat attgccgtgt ccaccgggtc ggcatgttcg tcggccgtcc ctgaaccctc
     6061 gcatgtttta ttgcgcatgg gcatttctaa agaattggcg aaagcctcat tgcgattcgg
     6121 acttggaagc gggaacacat cggatgacat agactatacg atcaagcgca taacagaaac
     6181 tgtcggcaaa ttaatcaaaa cgatataaac attaatatga tcatcgaaga taaaaaaaaa
     6241 gcattacgtt tttttcaata cggtatgttc attttgacag ccaaagcgca gcatgatttc
     6301 caaggaagcc cgtatgccgc ctcaaccgtg acttgggtaa gccaggcatc ctttgagccg
     6361 cctctgatca tgctgggttt acgcaaagac agttgggcca atgaggcggt acgccaaacc
     6421 gattcttttg cgctgaatat tttatcagaa gatcaaaaat caattgctgg aaaatttttt
     6481 aaaaacattg aagtaaacgg ccaaatgatc aatggatatt cgtttgatac ggatcaaagc
     6541 ggtgcgccta tattggatga agcgcaggcg tatatcgaat gtacggtaac cgatcgtatc
     6601 gaacggggtg atcattcgat catagtagcc tccatcatta atgccaaagt acgtcgtgat
     6661 tttaaaccta ttttattacg ggataccggt tggaattatg gcggttaaaa ggaatgtagt
     6721 tattatgcgt acgatagtta tgcttgcgat gtgtttttta gtcatcgttt cctgttcaaa
     6781 gccaaaagac agcgctcaac gcgatttgat gcgccttgga attgcataca acgatcagga
     6841 atttctgtcg cgcattcgaa aaggtgatct tattttgacc aatatgtttc ttcaagccgg
     6901 aatgaatcca aacatgagat ttaccaatca caaggattcg gtttcgcaag gcgagtccaa
     6961 tgaccctacg ccattgatga tcgcttcgcg tgaagggcat atcggtattg ttcgttcgct
     7021 gttacgcaag ggtgctgatc cgacggccgt tgccgataat gggcaaacgg cattgatgta
     7081 cggcgtgcgt tatgcaccga ctgaacttgt gtccgatctc atagtcgccg gcgccgatgt
     7141 aaatgggcgt gacaaagacg gtaaaagcgc actgaccttt gccgtattgc ttgatcgctt
     7201 ggatgttgta cgtcttttac ttgatcgcgg agcggatcct aatgcgatgg atcgtttttc
     7261 atttacaccg ctgatgcaag ctgcaatcaa aggtaaagcg ccgatcatgg aggctttatt
     7321 agacaaagga gcaaatcttg aagtgcgaac acccaacggg gaaaccgtat tgatgcttgc
     7381 ggttaactat aatcgaatgg aggctgttcg attattagtt aatcgtggcg ttgatttgaa
     7441 agccaaagat atgcaagggc gtacggcgct ttccatagcg cgcgctaaaa aacatgaaac
     7501 gattatcgaa atactgacga ctgctgaatc caaaaatcaa taaacataaa ctatcctgtg
     7561 aggtaacatg catttttatc gtatgttgat tgtaattttg tgctgtagtc tttcactgtt
     7621 tgcacaagcg gaaaaaggcg attcggtcgc cgccgaagcc ataaaaaccg aggcgataga
     7681 gcgctcgcaa gctctgcaaa ctctgaatgt tcttacaaat gtgatcggtg cacgtttaac
     7741 taattcaaaa ggattagcgg ccgctaacga ttggacgcgt gaggcacttg aaaaaatggg
     7801 cttatcggca gaagtcgaac cgtggggaga atttggtcgc ggatgggata tgaaaagttt
     7861 tcgtgccggc attgtggcgc cgtatgcgat accactcatc gcttatccca aagcgtggtc
     7921 acccgctacg cgcggcacgg ttcgatcaaa aatcttgtat atcaacgaaa cgaaactcga
     7981 agacattcag aagaaatatt caaacaaact caagaatgcc attgtacttt taggcgataa
     8041 ggtaaagatc gaggaagatt ttgaagcgga tgctaaacgc tggagcgata gtgaattatt
     8101 gaccatgtcc aatgccggaa aaaatgaagg acgagcagaa tcttttcgga ggagttttcg
     8161 taagtcgcct cgtttctcgg cttttattga aagacagagg atatttacat ggatcaataa
     8221 tcaacaaccg gcgcttatcg tggacggagg atcgtttagt tttcgcggcg gtacacctca
     8281 ttcgggcgta gtgcgtgtta tgggtgcatc catgccgctt gtggacgaat ccgatccgtt
     8341 cggtaatacg aatcccaaag cgtgggatcc taatccgcca tcggtagcac cgcagattgc
     8401 tatttctgcg gagcagtaca atcagctgat cgaattgatc aaaagcggta aagaagtgat
     8461 catggaatgt gatctccaag tcgagtggcg tgataaagat ttgaaagcgt ataatacgat
     8521 agggacttgg agcggaagtg atcgcaaaga tgaaactgtg atgatcgggg cgcacctgga
     8581 ttcttggcat tccagcaccg gagcgacgga taatgccgcc ggcgtttccg taatgatgga
     8641 ggctattcgt attctcaaag ctatcggttt tcaaccccgt cgaactatta aaatcggcct
     8701 ctggtcagcg gaagagcagg gtttactcgg ctcggcaggt tatgttaata aacatttata
     8761 tgattcgacc ggcgctaaac cggggtatga aaaattcaat gtctatttta acatggacaa
     8821 tggcggcggt caaatccgcg gcatttattt gcaagggcat gaagatgtgc agtcttattt
     8881 tagacaatgg atggaacctt tccgttcctg gggcgcatca accatttcac ttaaaggtac
     8941 cggtagtacg gaccacatgt cttttatcag cgccggatta aacggttttc agttcattca
     9001 agatcccatg gattatagta atatcacttg gcataccaac atggatatgt atgatcatat
     9061 ccagccttta gatttgaaac gcaatgcggc tattgtcgct tacttcgttt atatgagtgc
     9121 tatgtctgat gaagttttgc ctaagcgtgc gatagagaag gagtaaatag gtacattcat
     9181 ttttgaatct aatacctatc acaaagtttt aattgtttaa tacataaccg cttgataggt
     9241 caataaaaaa tgccggtaat attgattacc ggcattttta tttgtcgtta cagaacagtt
     9301 taatagatca aataacccat tccgatggaa taagtgatat tatttacttt agttttttta
     9361 acgacatcag gcacgccaaa ataaatgcga ccttcaaccg ttattttaga atcagctgtt
     9421 cccgtaccca aaccggctat aataccgtag tcgcttttct ttggtttggt taatacagtt
     9481 gctgaacctt cacgatcacg tgtgtaagct ttcttggcat agctaaacga cggtccacct
     9541 ataaagtaga acttttgttc ttcgatatcc atgccgacac gcattagaac aggcatttca
     9601 aaatattgat aataatattc gaagttttgc ccgggcgtgg ttgtccgtgc tccttttaat
     9661 gtataaagca attcgggttg aacggcaatc atgggcatta gttcatatag agcaaatacg
     9721 ccgacattat atccggtctt gccttttgtc tggtcattgt ctttaccgcc aaacaaagag
     9781 taattgaacc cgcctttgac gccgagtttg atatctaacc ctgcgatctg agagtgcaca
     9841 accgatagcg gcaccaagaa tgacacggcg atagccacgg tcattatttt aatatatttt
     9901 ttcatcgctt agaccttttg tttgatgaaa cactgtttta gaaagaataa cgcgcaccta
     9961 caagcacagc ccagcgtgac gctgtcgtgc tgatcgaata ccgatccggg cgtttagtaa
    10021 acgagaatac aggtttgccg gtgcctgatt ggatgccgcg gaattggatg gcttgatcat
    10081 tttgatttgc gatggatttt gattgacccc attcattatc aaaaatattc ataacattga
    10141 taacattggc ggttaattca aacttaccaa gttctttcaa cacaggaagt tcttgtgtga
    10201 tctggaaatc gacgcgattg gtccaaggtt cggtcgaagc attacgatct acgatcttac
    10261 cacgtgcttt acgcagggcc ggatcaccgc tgataaattc gtcaagctcc acccaaattt
    10321 gatccgccga gcgagtatcc gagttacttg taggtaccaa aacaatatcg tttttatctt
    10381 taggtacgta gatcaagtca ttaccggtat taccgtcagc attcacgtcg ccattgtagg
    10441 tcgtcgaata ggggcgaccc gaaaaaccgc tgtagaataa cgaaaagact gttttccact
    10501 ttggtgcaac ttcgatttct ttagccacgg atacaatgat gcgatggcgt tgttcgtaat
    10561 tagaagttgc agttttgacg ttattaggat cacccttaac tggattaaac tggaagttag
    10621 aaagcgcaat actgcttgtt gcactggtca tatcggtagc acggccatac gtatacgcta
    10681 cacttccaaa ataaccgtta tcatacaatt tttgcaattg aaccgaagca ttccactgat
    10741 aaccccattt ggtgtttgtc agataaataa cgttggtgta ttgtttggat ttgctaagcg
    10801 gaagaacacg ggcgggtgat gtggtcgcgc tgctactcgg gcttgtgtaa gtggaaattg
    10861 taccaaaccg aggacgatta tctgtaggtg aataactttg tgggcccgct aagctaatat
    10921 ctttgaacat aacgtcatta atgttatccg aacctacaaa ctcaactgta cctacccacg
    10981 tgtctgatag tttttgatca agagccaaat tgacacggaa tacctgcgga ttcttaaatt
    11041 ttggattcat aacattgatc gttgaacttc caagagtacc ggccgtaggt tgttttgtag
    11101 gatccttttg aaaagtcata ttggtcaaag tactgccgcc ggttaaacga atggttccga
    11161 gatcagcacc tgtattggaa tattgattgg aaatccagac ataggggttg cgacccgaga
    11221 aaattccgaa accacctcga aactgcaggg attgatcgcc attcacgtcc caattccatc
    11281 cgatgcgcgg tgaccacaaa atattaccgc tggcaatttt atccgtgcga tattttgcat
    11341 ttttgattgt tgtatcgttt tcgccaccgg tgtgataaaa acgtgcgtaa ttgatcgcga
    11401 aactgtcatt agcggtgggt ttgtctgaaa taataggtac gtcaaaacgt aaaccagctg
    11461 tgattgttaa gctaggcgtg actttgaatg catcttgaat ataaccaccg agctgcatta
    11521 cagagaactt cgcaagcgga acgcgatcgg tcgtatcggc attaaaaccg gttggatcgt
    11581 tgggatcaag agcaaaggtc agattgtatt cacgaatatt tcccgtaagg ccataatagg
    11641 atctcttaaa attttcaaaa ctgttgaaat cgtatcggcc attaaatgag ggaaggaatc
    11701 cgttctcaaa tttgaaatat tcgttgtgtg taccgattgt aaatgtatgc tcacccatat
    11761 agtaggtaaa attgtcggta aactcgaata cggattgttt tagcttgttg aactgagaaa
    11821 attgttctgt gccgatgacg taagaaagac cagcaaccga atcccgaatc ataacataag
    11881 gaaatcgatc acccggagtt tcgcgatggt catcaataac ctgataactc atggtgagct
    11941 cattaaacat ttctttagac aaagtgctat tgagctgtgc gaccgtcgag ttcgttatgt
    12001 ttttgaaaat atagcggctg ttggaaaaac ggaaaccgcc tgaactggag ctactgttcg
    12061 tgcctgtctc acggcggaat aaatcttgat tggcattgat gtagttgtgc cgaatagtaa
    12121 accgatgttc cggtgtgaga ttgaaatcta atcgtgcgaa aaacttgtag ctttcagtag
    12181 gaatggtgct cataccaaaa gaaccgggat catgaagtac ggtatctttg agaaaatttt
    12241 gtatatctct cgctttttta acagagaggc gactaccggt tcccggaaaa tcaagcgaag
    12301 aaccgtcgcc caaaataccg tagttggttg gaacttcttg cagttcgtgg tcataattga
    12361 caaagaaaaa catcttatct tttataatcg gaccgccaag acgtgcacct tccgttcgaa
    12421 taaagttttt cgtgggaaga acaccgtcaa aactttcgcc ttgaaaattt tcattgcgat
    12481 agttgtaata actagaaaac tcaaatttat tggtaccgct acgggtaatt gcattgacgt
    12541 tgcccccagt aaaattgccc aagcgcacat cataaggagc aatttgtact tgaaattcct
    12601 gaatagcatc aagactgatc ggtgctgtac cggcttgtcc gcccggcgta ccgctggccg
    12661 ctaaaccaaa gagatcgttt gataccgcgc cgtctatcga aatattatta tagcgattgt
    12721 ttcggccggc cgcgctattt ccggaaaatt ggggtgtgta tttcgtgaaa tcattgaaat
    12781 cacgggtaac cgcaggaaga tatttcacct gaagctcatt gacggtagtc gcagcgccgg
    12841 tgcgcgcatc gttgataatg gggttacgct cggcaacgat ttccgaacct tccatttcta
    12901 ccgcttcttc aactaataca aacgtaagat ttttctcctc gccgagagca agattgatgt
    12961 tttcaatttt gtgctctttg tatccgatca tcgaagcggt gaccgtataa ggaccgccga
    13021 cacgcatgcc cgggaggtta aaccgcccgt ccgaacggct ataggctccg gctttggtgc
    13081 ctgaaggttc gtgcacagcg ataatattga cacccggaag tggagtgccg ttgttatctt
    13141 tgaccatacc gctgatggca gacgtggtca cctgagcgcg gaggttaagt gtaagggcaa
    13201 ataaaaacat cgccgcatac acaaaagtac cacgccaatt acgagagtaa aaactacctc
    13261 tcatcctgac ttctccttat tagttgagat tgggacatcg tgaagaattg attaaatatt
    13321 aagaacaaat actttaaaag aaatgaacta aacgaggtaa tatgggactg atttgttgcg
    13381 gaaatattag gtatttgtta ttacttccgc aagaattttt ttgaggagaa tttattgttt
    13441 tatattcata attcaccttg aatttgtaaa cctcgttttg tactatgcac gcgctgtttt
    13501 tgtttgcgcc cgtagctcaa ttggatagag tgttggcctc cggagccaaa ggttgtgggt
    13561 tcgattcccg ccgggcgcac agcttatttc ataatgcatg ataaatacag acttttactt
    13621 ttttcaataa tcgacctagt acaacttgaa ataggaaaca cttttaaacg tattacgaat
    13681 aacgtactaa tttccaaaag ctgtttccgg tgtctccgat aacccgaaac agttttttta
    13741 ttttatcgaa ggaatctacc tatggctgaa cgaattacta aacgcgaaca agattattca
    13801 cagtggtacc tggatgtcat tgcgcaggct caacttgccg attatgcgcc ggtcaaaggt
    13861 tgtatggtaa ttcgtccaaa tggttacgcg atatgggagt tggttcaaca atcattagat
    13921 aaaatgttta aagataccgg ccatgttaat gcctactttc ctctattcat accggaaagt
    13981 ttcatgacta aagaaaaaga gcacgttgag ggtttttcgc cggagtgcgc ggtagtcacg
    14041 catggcggcg gaaaaaaact ggaagaaagt ttgtatgtac gaccgacgtc ggaaacaatc
    14101 atttggagta tgtacaaaaa ctggattcat tcctatcgcg atctgccgat tttgatcaat
    14161 caatgggcga acgtcgtgcg atgggagatg cgtacccgat tgtttttacg aacgacggaa
    14221 tttctatggc aggaggggca tacggcgcat gcgacgtatg atgacgcgga aaaagaagct
    14281 atgcagatgc tgcatgtgta taagacattc gctgaagagt atatggcatt gccggtttac
    14341 cacggaaaaa aaaccgattc agaaaaattt gccggcgctc tgcataccta ctgtatcgaa
    14401 gcgatgatgc aagacaaaaa ggcgcttcaa gccggtacgt cgcataatct ggggcaaaat
    14461 tttgccaaag cgttcgacgt gaagtttcag gatgagaacg cacagcttca gcatgtatgg
    14521 gcaacaagct ggggtgtaag cacccgtctg atcggtgcgc tcattatggc ccacagcgat
    14581 gataacggat tggttttgcc gcctaaattg gcaccgatgc aagtggtgat tgttccgata
    14641 tataaagaag atcatgaacg tactgcgacg gtggaagccg cgcaaaagtt gaaatccgac
    14701 ttgcaatcca aaggcattcg cacgcatgtg gatgaccgtg atcagtatcg ccctggtttc
    14761 aaatttaacg aatgggaagt caaaggcgtg ccgatccgcg tcgaactggg cccgaaagat
    14821 ttagccgccg gccagattgt atgcgcacgt cgtgataaaa cggccaaaac ggataaaatc
    14881 acgatggcgg tcaatgaagc ttcgtcacgt attcctgcgt tattggaaga gattcaaaaa
    14941 aatctatttg atcgagcaaa agaatttcgc gataaaaata caattttcgt cagcgattat
    15001 gccggattaa aaaagatcgt tgaaaacgaa gaaggttttg cgcgttgctt ttgggcgggt
    15061 tcccgcgaag atgaagccaa gatcaaagaa gaaaccaagg ccacgatccg atgcataccg
    15121 atggagcagt ccggagaaaa aggtcgctgt gtgttgacag gtaaagaaac gaatgtgcag
    15181 gttattttcg ctaaagcgta ttaatgatcg cttaaacaga gggcatcgct atgaatagtg
    15241 aaattaaatt tcattgtaag gatcaaggta gcggtgtacc cgttgtattt atacacggct
    15301 acccgcttga tcattcgatt tggcaaaatc agtttgagtt gtcggatacg tatcgaattt
    15361 ttgctttgga ccttcc
//
LOCUS       DAOQNQ010000046        15295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1375_length_15295_cov_5.806824, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000046 DAOQNQ010000000
VERSION     DAOQNQ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15295
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1375_length_15295_cov_5.806824"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PK754_04845"
     CDS             <1..704
                     /locus_tag="PK754_04845"
                     /inference="COORDINATES: protein motif:HMM:NF028180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08861.1"
                     /translation="FIASCKSKWSLSTSLVMLLPHGYEGQGPEHSSARLERYLQLCAE
                     DNMIVCNLTTPAQYFHALRRQIRQKTEKPLILMTPKSLLRLPDARSYKHEFTNGTFHE
                     ILDDATVTDRQAITRVIISAGKVHYDLVKFRIKNNRTDVALVRLEQIYPYDADTMKRI
                     LASYPNAATVAWTQEEPRNMGSWNFLLERLTNDLTAKQKLVYAGRKASASPAVGSLSV
                     HQAEQEQLVRDAFEL"
     gene            746..2152
                     /locus_tag="PK754_04850"
     CDS             746..2152
                     /locus_tag="PK754_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNB08862.1"
                     /translation="MQDYRFSELFEAQKKHFQSHIKSSRPDARIVKLKKIRTWITAHA
                     QDIRDAIYEDFRKPSVEVDLTDIRPVLMEIEHALVHIRSWMASRYVSTPLLFFGTRSK
                     VVYEPKGVCLILAPWNFPFMLSVGPLISAIAAGNCCILKPSEMTPQTSRLIARMIAEL
                     FNPNEVCVVEGDHVAAEALLKLPFHHIFFTGSPAIGKKVMHAAAEHLSSVTLELGGKN
                     PVIVDETADIEDTVSKLLWGKFMNGGQSCMAPNYVYVHHTIYTAFCEALVAMYARMFG
                     TANEPSPDVAGLVSDRHYERITSLIRTTVAQGAKTLAGNHFDAARRYVAPTVLGNVTT
                     DHAVMQDEIFGPILPLLRYTHRDEVIAYINTQERPLNLYIFSTDMAKADAIIAQTTAG
                     STCINETTLNFAHPDLPFGGVNTSGIGKAHGYYGFLAFSNERGILKQRRGLTTMKLVY
                     PPYTERVKKIVKLVMKYL"
     gene            2237..3145
                     /locus_tag="PK754_04855"
     CDS             2237..3145
                     /locus_tag="PK754_04855"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNB08863.1"
                     /translation="MKQTKNFPLRVLTFNVWGLKVGPLHIARDIKARMDRIASALHRS
                     ESDVIVLQEVWCASIAKFLIRESGYPYHVYQPGRGTLRGPLGNGLLMLSRFPIVHTQT
                     LRFHHCTSPDEWFVGKGAVAADIHTAQGVVRVINTHLGSGKRPLHVTMRLQQLRQLQS
                     WVAGLEHHLPSVIAGDFNFSPSSLEYCVFRHWVTHHFRESLADTFAVRHGQADGHTYF
                     VDRSYHVKPKIHDRNERIDYVFLLTPKQSRLDLTVEESTRILNDAEHPYSDHCAVLST
                     VGIRIKPTLREIMVSDPMIPARKAIL"
     gene            3142..3780
                     /locus_tag="PK754_04860"
     CDS             3142..3780
                     /locus_tag="PK754_04860"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNB08864.1"
                     /translation="MSELQTVITVLSGDFETYGYAGLFGLLILTTILPLPLPEELMCI
                     TTGYLIERGVMAPVPAILTMFMGIVASDVYQYYFAQILGNKLMRVRPFSWILNEERIR
                     KAEALFLRQGSKVLFFGRWVYGLRSNIHFAMGLLRFPIRRFIVIDAAVVSIQSLSLVL
                     IGYYSPLYMNHWASHLMTLQWWLYPLSMIALSVALSGMIFKAWKKRQREVIL"
     gene            complement(4005..>4463)
                     /locus_tag="PK754_04865"
     CDS             complement(4005..>4463)
                     /locus_tag="PK754_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08865.1"
                     /translation="LSSFMKRWGGTWFLIGTVANTVVGFMFYFAIPETLRTAFTEGTG
                     QMLFLSSHVLFIIGTLAIAAVTFLKANNMIGWVGIAAMLVGIAEKVISRDLLRQEYLK
                     QVNWTHAVLQTKPQNDVIALFFISFVAGIGLLLWVYMRYKKEVAAHTQQS"
     assembly_gap    4464..4563
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4564..4934)
                     /locus_tag="PK754_04870"
     CDS             complement(<4564..4934)
                     /locus_tag="PK754_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08866.1"
                     /translation="MQNQLPIPHPDPLGVPGDIGLILFLLYLTFFLHVILMNALLGGA
                     FMAVISEWISRKTNNQQTKDHHIHLAEKLGHILPFSVSLTVSMGIAPLLFVQVIYGQF
                     FYTSSVLMAWIWLSVVFLIVIG"
     gene            complement(4951..6327)
                     /locus_tag="PK754_04875"
     CDS             complement(4951..6327)
                     /locus_tag="PK754_04875"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNB08867.1"
                     /translation="MPEYPVWNVGGVGHGWIIGLIAMTHVYISHFAIGGGLYMAVTEA
                     LAVKRNDQAMIGYLRSHSNFLLLLTTVLGAMGGIGIWFAVGLVNPASIQALIHNFMFF
                     WAIEWVVFVVEIAIAFFYYYSWDKVSPQFHVKLAWLYFIISAFSLLVINGILTFMLTP
                     GKWLETGNVWDGFFNPSYFPSVIVRFLIMCTIAGVFSIFTSSRVKDENLRTRLLHYSA
                     KWLMPSFFLLPFFLYWYFTSLPADVAKLLETGIANIGAGNLSVLTRVLLLTMLLSVTI
                     YVIALVGPFFNPKIFTFGFSVAILILGLIVTGATESIREMIRKPYTVYGYLYSNGTLK
                     KASADSSNYGILKHYPWIAEKEITPANMKTVGQKIFLAQCQSCHTPTGYRSMKDLAKG
                     WDREFIFRRLTTLPATGIMPPFMGDEQERRALADYIGDIVGAGPWPPPDSTPATTPAP
                     APTASIKN"
     gene            complement(6357..7463)
                     /locus_tag="PK754_04880"
     CDS             complement(6357..7463)
                     /locus_tag="PK754_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNB08868.1"
                     /translation="MSNEMANKNHDDPNIAFLPKFIRERYDVGWIVAFFKHKEVPVHK
                     HSLWYYMGGISLFLFLVQIVTGVLLMVHYNAGEAHASVQAIMSQVDFGWLIRSTHSWS
                     ANLMVFFVFLHMFSVFFMKSYKYPREMTWMTGMILLAMCLGFGFSGYLLPWDQLAYFA
                     TQIGVKTTEAVPVMGGFIADMLRGGKELGTQTIERFYALHVWVLPALFMMTLGLHLLF
                     VQLQGIHAPEFFENQPADKKKYMKFFPDFAIKDVYVWLIVMNFLGLLAVMFPWELGAE
                     ADPLAPAPAGIKPEWYFLAMFQLLKLFPPEIGPIEGELFGIMLFSLVGVLWAAIPFID
                     SRTAPAWRSRATFYFGIAFLTGFIIFTMWGHYSK"
     gene            complement(7488..7955)
                     /locus_tag="PK754_04885"
     CDS             complement(7488..7955)
                     /locus_tag="PK754_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HNB08869.1"
                     /translation="MKDTKDPSEHIHMPDKRDFMRTVFTGGFMLWIGAFLYPVYRYLK
                     PRVEEDTANAVQSVVAGKVADFDKVRAKLIKFGSKPVLLLKNDAGELVALGATCKHLG
                     CTVQYASDKNHIFCGCHGGIYDLTGKNIAGPPPAPLDKFKVVTNGDEIVIHRV"
     gene            8216..9304
                     /locus_tag="PK754_04890"
     CDS             8216..9304
                     /locus_tag="PK754_04890"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019991158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNB08870.1"
                     /translation="MDRKISFAPVQGAFSQVLKERVQQYFAEKGISMRGGLHMAIKSG
                     FYLSLAVMTYILIISGILSVWGLLLAAMLMGFLVACIGFNVGHDAIHGAMSHRKWVNT
                     LMGLTFEIFGASVYLWRMYHNVIHHTYTNIPGADRDIDGRPLIKLAPSQPTYKIHRFQ
                     YIYAFAVYTLTSLNWVLRKDYQRMFMKHNGVYTTKMPSVPLIASMFMFKLVHFTLFLI
                     LPIIVLPVPFWMVIIGFFVMHMTTGITLALVFQMAHVVEGMEFPEPHEDGMLENEFFV
                     HQLKTTANFSRKSKIATWICGGLNLQVEHHLFPHICHTHYPAISDIVKRTAQEFGIPY
                     HENTSFWSALASHVRHLRKVGYIPVQLG"
     gene            9465..10310
                     /locus_tag="PK754_04895"
     CDS             9465..10310
                     /locus_tag="PK754_04895"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB08871.1"
                     /translation="MNTYLKNIVEYYHATEKAYHLHWDVKNSLSVHYGYWDDKVSSFS
                     ESLLRMNEVMAEAVGITSSDRVLDAGCGVGGSSIYLAKTRGCRAVGITLSDRQVAQAE
                     TFAKDRGVSEQTEFKIMDYCHTDFPDASFDVVWGCESICYAESKEQFIREAYRLLKPG
                     GRLVIADGMTAKFENNDHPINRRWLEGWAANFIETPERMQGYMQHMGFTDIRFRDITP
                     HVMHSSRRMYMLYFPAKVYHAVVRMLGRPSPLLFRKNVDACRFQYIAFKRKLWMYGLL
                     CGVKK"
     gene            10323..11171
                     /locus_tag="PK754_04900"
     CDS             10323..11171
                     /locus_tag="PK754_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB08872.1"
                     /translation="MSNYHDQIVDYYRETENHYRVYWALDKTLAIHYGYWDEKVKSFP
                     QSLLRMNEVLAETAKIQKTDRVLDAGCGVGGSSIFLAQHIGCHATGITLSAKQVARAF
                     ENAKDKNVSDRTDFKVMNYCKTDFPDASFDVVWGCESICYADSKEQFVREAFRLLKPG
                     GRLILADFMVPAFENNQEPSVRRWLDGWVVNFLETPDNFKKYMLEAGFEDVRFRDIST
                     HTLHSTKRLGRLYYWGKVYLAYCKIIGKTMDPIRLRNIKSAYDQYQSHRRALWIYGMF
                     CTTKPS"
     gene            11237..12082
                     /locus_tag="PK754_04905"
     CDS             11237..12082
                     /locus_tag="PK754_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB08873.1"
                     /translation="MSAYHQNIIAFYDVLENPLRMIWDLDKSYAIHYGYWDEKVKSFP
                     ESLLRMNEVMAETAQIKSHERVLDAGCGVGGSSIFLARTIGCKTVGITLSANQVQRAK
                     KNAQNSGTAHLNEFKVMDYSHTDFEDASFDVVWGCESICYADNKEEFVKEVYRILKPG
                     GRFIMADGMVQKFEDNDNPIVAKGRDGWAANYVETPERFKQFLENTGFTNITYRDITD
                     HIMHSAKRLYWVSFPAKLNQWIRPLFGKTVAEHNKGNVNACKYQYLAFKKRLLVYGIL
                     CGVKR"
     gene            12116..12964
                     /locus_tag="PK754_04910"
     CDS             12116..12964
                     /locus_tag="PK754_04910"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB08874.1"
                     /translation="MNSYQQKIVQYYHATEKSYKDGWGLKKNLMIHYGYWDDKVSSFS
                     ESLLRMNEVMAEAVGITSSDRVLDAGCGVGGSSIYLAKTRGCRAVGITLSDRQVAQAE
                     TFAKDRGVSEQTEFKIMDYCHTDFPDASFDVVWGCESICYAESKEQFIREAYRLLKPG
                     GRLVIADGMTAKFENNDHPINRRWLEGWAANFIETPERMQGYMQHMGFTDIRFRDITP
                     HVMHSSRRMLYFYFPTRCYHLYQYLIRKSVPEVNRKNIDALLYQYIAFKKKLWLYGML
                     SGVKHQ"
     gene            complement(13043..13678)
                     /locus_tag="PK754_04915"
     CDS             complement(13043..13678)
                     /locus_tag="PK754_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08875.1"
                     /translation="MNRRGFFNNIFKSDEPSGPIHFYGIQIVINTGINDETRAKLHAA
                     INVPVKEGETPEEKKAFFKNVAALLLENEPFFEYGYWDYLTDAAEAQAEFDSWVNEIE
                     SSMATESGEVGETMDEQYRMSAEKQYVVISLAFLIENVPTQESFQGMIDIPEESYFLR
                     ETWPKLIEAVPYLDFNYVMADAVFIMPGNEKDGFSFEDLHSEGWEYLKPIS"
     gene            complement(13696..15228)
                     /locus_tag="PK754_04920"
     CDS             complement(13696..15228)
                     /locus_tag="PK754_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08876.1"
                     /translation="MAKRKTKRNQKAQTPAWWNAMISRREAGQRLATLATGAAMLPYA
                     SILTGCGEEVELDALELQQKQGWQVGADGENLDFKDEQSRDSLGQAWENYADPAALMV
                     AYEPKNYRWKPFKVTTLVQSLSQETLRKQVKPVLSDAMKTAYARGLGMREIIQKMDNP
                     SSTMIIADLHGEESVAFGAALADVANVVIGFDNWPHPQGVVPAHRTLGAMMYYAREVS
                     VKTEEKLSWDAPAVILLDNHRLGEYTDEWDVFDNRYVAKVPSAPSLASYKVNRVLYVI
                     PDRDTKNELDDLNDDFVYYKEKNISVDLLPMSDFKPAVASAMATSASAKTSADSMIAA
                     FTGSDSAIAKAMTAPAQKDTVVATPASTRTVYHYGGGPSYWPIFFGNYNTPSTISRPG
                     GYRAPSQGPSKPEYKPERRPTLFSSRTVGSQTSGVGKQKPTGFGRVSANVNSDGNVTR
                     VRQSRNYSSTGTSRASRSTSTSSSSRSSRSYRSSSSKSSSSRSSSGRSGSFGRSRSSS
                     SS"
BASE COUNT         4008 a         3382 c         3784 g         4021 t
ORIGIN      
        1 attttatcgc gagctgcaaa tccaaatgga gtctttctac atcgctggtg atgctgctgc
       61 cgcacggcta tgaagggcag ggccctgagc actccagtgc acgtcttgaa cgttacctgc
      121 aactctgtgc ggaggataat atgatcgttt gcaatctgac gacaccggcg cagtatttcc
      181 acgcgttgcg gcgccagatc cgccagaaaa cggaaaaacc gttgatcctt atgacgccca
      241 aaagcctcct gcgtttgccg gatgcgcgtt catacaaaca cgaatttaca aacggcacgt
      301 ttcacgaaat attagacgat gcgacagtta cggatcgcca agccataacg cgcgtgatta
      361 tttcggccgg caaagtgcat tatgatctgg tgaaattccg gattaaaaac aatcgcacgg
      421 acgtcgcgtt ggtacgtttg gagcaaattt atccgtacga tgcggataca atgaaacgta
      481 tcctcgcgtc ctatcccaat gcggctacgg tggcgtggac gcaggaagag ccgcgcaata
      541 tgggttcgtg gaattttctg ttagagcgac tgacgaatga tctgacggcg aaacaaaaat
      601 tagtttacgc cggacgcaaa gccagtgcaa gccccgccgt cggttcgctc agcgtgcatc
      661 aggccgagca ggaacagctt gtgcgcgacg catttgagct gtaatgtggt ttatttttta
      721 acgttgttat agacagggct tgaaaatgca ggattatcgt ttcagtgaat tatttgaagc
      781 gcagaagaag cattttcaaa gtcatatcaa gtcatcccgc cccgacgcgc gtatcgtcaa
      841 actcaaaaaa atccgcacat ggatcacagc gcacgcgcag gatattcgcg atgcgattta
      901 cgaggacttt cgtaagccgt ccgtcgaagt ggatctgaca gatatccgtc ccgtgttgat
      961 ggagatcgag catgccctcg ttcatattcg ttcctggatg gcgtcccggt atgtatccac
     1021 ccctctgctt tttttcggta cacgatccaa agtcgtgtat gaacccaaag gcgtatgcct
     1081 gattttagcg ccgtggaatt ttccttttat gttgtccgtg gggccgctta tctccgcgat
     1141 cgcggcgggt aattgttgta ttttaaaacc ttccgaaatg acgccgcaaa cgtcccggct
     1201 tattgcccgc atgatagccg aattatttaa tcccaatgaa gtgtgtgtcg tcgaagggga
     1261 tcatgtggcc gcggaagcgt tgttgaaatt accttttcat catatttttt ttaccggcag
     1321 tccggccatc ggaaaaaaag taatgcacgc ggcggcagag catctcagtt ccgtaacgct
     1381 ggagcttggc ggaaaaaatc cggtgatcgt ggacgaaacg gcggatatcg aagatacggt
     1441 ttccaaattg ttgtggggaa aatttatgaa cggcggccag tcttgtatgg cgcctaatta
     1501 tgtgtacgta catcatacga tttacacggc attttgcgaa gcgctggtgg ccatgtatgc
     1561 acgtatgttt ggcacggcga acgaaccctc gccggatgta gccggtcttg tgagcgaccg
     1621 tcattatgaa cgtatcacct cactgatccg cacgaccgtg gcccagggtg cgaaaacgct
     1681 tgcgggaaat catttcgatg ctgcgcgacg ttatgtcgcc ccgacggtgc ttggtaacgt
     1741 tacaacagat cacgcggtga tgcaggatga aatttttggc ccgatacttc cgctgctgag
     1801 atatacgcat cgcgatgaag tgatcgccta tatcaacacg caggaacgac cgctgaattt
     1861 gtatattttc agtacggata tggcaaaagc tgacgcgatc atcgcacaga ccacagccgg
     1921 ttcgacatgt atcaatgaaa cgacgctcaa ctttgcgcat ccggatttac cgttcggcgg
     1981 tgtgaatacc agcggtatcg gcaaagcgca cggatattac ggatttctcg ctttctccaa
     2041 tgagcgcggc attctcaaac aacggcgcgg tctgaccaca atgaagcttg tgtatccgcc
     2101 atacacggag cgcgttaaaa aaatcgtcaa attggtaatg aagtatttat aagttatttc
     2161 tcatttaatc tcttcgtaac gaaaacattc tattctcata acatgcgtgt tgtttcttta
     2221 aaagaaaaca ccgcgcatga aacaaacaaa aaatttccct ctccgcgtac tcacgtttaa
     2281 tgtctgggga ctcaaggtcg gaccgcttca catcgcacgt gatatcaaag cacgaatgga
     2341 tcgcatcgct tccgctctgc atcgatcgga atcggatgtc attgtgttgc aagaagtatg
     2401 gtgcgcatcc attgccaaat ttttgattcg agagtcgggt tatccgtacc atgtctatca
     2461 gcccgggcgg ggtacattgc gcggaccttt gggtaacggt cttttgatgc tttcacggtt
     2521 tccgatcgtg catacgcaga cgttgcgatt tcatcattgt acgtcgccgg acgaatggtt
     2581 cgtcggcaaa ggtgcggtag ctgcggatat acatacggca cagggcgtcg tgcgtgtgat
     2641 caatacgcac ttaggatccg gtaaaaggcc gttgcatgtc acgatgcgtc ttcagcagct
     2701 acgccagctc caaagttggg tggccgggtt ggaacatcac ctgcccagcg ttattgccgg
     2761 agattttaat ttttcacctt cttcattaga gtattgtgtt ttccgacatt gggtcacgca
     2821 tcattttcgc gaatcgcttg cggatacttt tgccgtgcgg cacgggcagg cggacggcca
     2881 tacctatttt gtagatcgct cttatcatgt caaaccgaaa atacacgatc gaaatgaacg
     2941 tatagattat gtttttttac ttacacccaa acaaagccgg cttgatctaa cggtggagga
     3001 gagtacacgt attttgaatg atgccgaaca tccgtattcg gatcattgcg ctgtgttatc
     3061 aaccgtgggt atccgaataa aaccgacgct gcgggaaatc atggtatccg atccgatgat
     3121 tccggcaaga aaagcgattt tatgagtgaa ctgcaaaccg tcattaccgt attaagcgga
     3181 gattttgaaa cctatggtta cgccggttta ttcggattat tgattttaac aacgatcctt
     3241 cctttgccgt tgccggaaga gctgatgtgt atcacgacgg gttatcttat cgaacgcggt
     3301 gtgatggctc cggtacctgc cattttgacg atgtttatgg gtatcgtggc gagcgatgtg
     3361 tatcagtatt attttgctca gatattagga aacaaactca tgcgtgtgcg gccattttca
     3421 tggatactca atgaagaacg catacgtaaa gcggaagcgc tttttttacg ccaggggtct
     3481 aaagttttat tttttggacg atgggtgtac ggattgcgtt caaatataca ttttgcaatg
     3541 ggtcttcttc gttttccgat tcgacgtttt atcgtcatag atgcagcggt tgtgagcata
     3601 cagtcgttgt cgcttgtgtt gatcggatat tacagtccgt tgtatatgaa ccattgggca
     3661 agtcacctta tgactttgca gtggtggctc tatcccctga gtatgattgc tttaagcgtt
     3721 gccctcagcg gtatgatttt caaagcatgg aaaaaaagac aacgcgaagt gattttataa
     3781 aagtacggcg tttcttattg agagatcgca tatttgtatt gtcattgtca acgaaacaat
     3841 agcgtcgtaa gaacacatgt aaattcaatt atgaagaaat tactactttt ttttgcgcct
     3901 tagcgtctta gcgaacttgt attcagtctc gcaaagccgc aaagagaagc gggaatgaag
     3961 ttttttataa aaaaaagccc gacattgtat cgggcgtttt tcgtttaaga ttgttgcgtg
     4021 tgcgcagcga cttctttttt gtatcgcata taaacccaaa gtagcaaccc gataccggcg
     4081 acaaaggaaa taaaaaagag cgcgatgaca tcgttttgcg gtttggtttg taataccgca
     4141 tgcgtccagt tgacttgttt cagatattcc tgacgcagca gatcgcggct gatgactttt
     4201 tctgcgatac ccacgagcat cgcggcgatg ccgacccatc cgatcatatt gttcgctttg
     4261 agaaacgtta cggccgcaat agccagtgtt ccgatgatga ataaaacatg actggaaagg
     4321 aataacattt gtccggtgcc ctccgtgaag gctgtacgca acgtttccgg gatggcaaaa
     4381 taaaacataa atccgaccac ggtattggcg accgtgccga tgagaaacca tgtcccgccc
     4441 caacgtttca taaacgaaga aagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnccgatga cgatcagaaa cacgacggac agccatatcc acgccatcag tacggacgag
     4621 gtataaaaaa attgcccgta tatcacttgt acgaaaagca acggtgcgat gcccatcgat
     4681 accgtgagcg aaacgctaaa tggaagtata tggcctaatt tttccgcgag atggatatgg
     4741 tgatctttgg tttgctgatt atttgttttt cgggaaatcc attcggatat caccgccatg
     4801 aatgcgccac caaggagcgc attcatcagg atgacgtgta aaaaaaacgt gagatacagt
     4861 aaaaacagaa tcaatccgat gtcgccgggt acgccgagcg gatcgggatg aggtatgggg
     4921 agttggttct gcatacgcat gattcctggg ttagtttttg atgctggcgg tcggtgcagg
     4981 cgccggcgtt gttgcgggtg tgctatccgg tggaggccat ggacccgcgc ccacaatatc
     5041 gccgatatag tccgccaatg cccggcgttc ctgttcatca cccataaaag gcggcatgat
     5101 gcccgtcgcc ggcagcgtcg ttagacggcg aaagataaat tcacgatccc agcctttggc
     5161 caaatctttc atcgaacgat aaccggtcgg tgtatgacag ctctggcact gcgcgagaaa
     5221 tattttttgc ccgaccgttt tcatattggc cggcgtgatt tctttttcgg cgatccatgg
     5281 ataatgtttg agaataccgt aattggaaga atccgccgaa gcttttttca aggtgccgtt
     5341 ggaatacaaa taaccgtaca cggtgtacgg cttacgaatc atttcacgaa tactctccgt
     5401 ggcgccggtg acgatcaaac ccagaatcag tatcgccacg ctaaatccga aagtgaagat
     5461 tttcggattg aaaaacgggc cgaccaatgc gatcacgtag atcgtcacgg ataaaagcat
     5521 ggtcagcaaa agtacgcggg tcaacacgga aagattgcct gcgccaatat tggcgatgcc
     5581 cgtttccaaa agtttggcca catcggccgg gaggcttgtg aagtaccagt ataaaaagaa
     5641 cggcagcagg aaaaacgaag gcatgagcca cttggccgaa taatgtaata agcgggtgcg
     5701 taaattttcg tcttttacgc gcgaagatgt aaaaatcgaa aacacaccgg caatcgtaca
     5761 catgatcaaa aaccgaacga tgacggacgg gaaatatgaa ggattaaaaa atccgtccca
     5821 cacattaccg gtctcaagcc atttaccggg cgtcaacatg aacgtcagaa taccattaat
     5881 caccaagagg gaaaacgcgg agatgataaa atacaaccat gctaacttca cgtgaaattg
     5941 tggcgagact ttgtcccagg agtagtaata aaaaaatgca atggcgattt cgaccacaaa
     6001 gaccacccat tcgatcgccc aaaagaacat aaaattatga atcaaagcct ggatcgaagc
     6061 gggattgacc aatccgacgg caaaccagat accgatgccg cccatcgcac cgagtacggt
     6121 cgtaagcaaa agcaaaaaat tagaatggct tcgaagataa ccgatcatag cttgatcatt
     6181 gcgcttgacc gctaacgctt ccgtgacggc catatacaat ccgccaccga tagcaaagtg
     6241 ggaaatatag acgtgcgtca tggcgatcaa tccgatgatc catccgtgtc cgacgccgcc
     6301 cacattccat acgggatatt caggcatata cgagcccttt cattgaaata aaattttcat
     6361 ttcgaataat ggccccacat cgtgaagatg ataaagccgg tcaaaaatgc gataccaaaa
     6421 tagaatgtcg cccgtgaacg ccatgccggc gccgtacgcg aatcaataaa gggtatcgcg
     6481 gcccaaagca cgcctaccaa actgaagagc atgattccga aaagttcgcc ttcgatcggt
     6541 ccgatttccg gcgggaatag tttgagtagc tggaacatgg cgaggaaata ccattccggt
     6601 ttgatacctg ccggagccgg cgctagagga tcggcttccg cgccgagttc ccagggaaac
     6661 atcacggcca gtaagccgag aaaattcatc acgatcagcc acacatatac gtctttgatg
     6721 gcaaaatcgg gaaaaaactt catgtacttc tttttgtcgg cgggttgatt ttcaaagaac
     6781 tccggtgcgt gaatgccctg aagttgaacg aaaagcaaat gcaaacccag tgtcatcata
     6841 aaaagcgccg gcagcaccca cacgtgcagc gcgtaaaagc gttcaatggt ttgcgtgccg
     6901 agttccttac caccgcgcag catgtccgcg atgaacccgc ccattaccgg gacggcttct
     6961 gtggtcttca cgccgatctg tgtggcaaaa taagccaact gatcccacgg cagaagatac
     7021 ccgctaaatc cgaagccgag gcacatggcg agcaaaatca tgcccgtcat ccacgtcatt
     7081 tcccgcgggt atttgtagga tttcataaaa aacacactga acatatgcag aaatacaaaa
     7141 aagaccatga ggttggccga ccaactgtgt gtggagcgaa tcaaccagcc gaagtctact
     7201 tgcgacatga tggcttgtac gctggcgtgg gcttcaccgg cattgtagtg aaccatcagc
     7261 agtacgccgg tcacgatctg aacgagaaat aaaaataatg agatgccgcc catgtaatac
     7321 cacaaactgt gcttgtgtac gggcacttct ttatgtttga aaaaagcgac tatccagcct
     7381 acgtcgtagc gttcccgtat gaatttggga agaaacgcaa tattcggatc gtcatggttt
     7441 ttattggcca tttcgttgga catagttttt ttgcgtttag tgatggatta aacccgatga
     7501 atcacgatct cgtcgccgtt ggtgacgact ttgaacttat ctaaaggagc cggcggcggc
     7561 ccggcaatgt tttttccggt aagatcatag atgccgccat ggcatccgca aaaaatatgg
     7621 tttttgtccg aagcgtattg caccgtacac ccgagatgct tgcaggttgc gccaagcgcc
     7681 accaattcac ccgcatcgtt tttcagcaag agtacgggtt tgcttccaaa tttgatcaac
     7741 ttggcccgga ctttgtcaaa gtcggccact ttaccggcga cgaccgattg tacggcattg
     7801 gccgtatctt cttcgacccg cggtttcaga taacgataca ccgggtacaa aaatgcgccg
     7861 atccagagca taaagccgcc cgtaaagacc gtacgcataa aatcccgttt gtcgggcata
     7921 tgaatatgct ccgaaggatc cttggtgtct ttcatagaca ggttctcgcg ttaagttgtg
     7981 aaacaattat gtagaaagat tggactgatt cagatcaaaa gaagcgcgtc aatatagata
     8041 ggcgcagcgg atgacgcaat aattttttag gtgcatgggg cggttaaatt aattttattt
     8101 ttctacttga gattttaaat ataatttatg actattacgc gtcctacaag cgacgcgatg
     8161 acactgcttg atataatcgg tttcactaac caatcataaa tagaaagttt ttccaatgga
     8221 tcgaaagatt tcctttgcac cggtacaagg cgcttttagc caggtactca aggaacgcgt
     8281 tcagcaatac tttgctgaaa aaggcatttc catgcgcggc ggtttgcata tggctatcaa
     8341 aagcggtttt tatttgagtt tggctgtgat gacttacatc cttataattt ccggtatttt
     8401 atcggtatgg ggacttttgc ttgcagccat gcttatgggc tttttagtag cctgtatcgg
     8461 cttcaatgtc gggcatgacg ccattcatgg cgcgatgtcg catcgtaaat gggtcaacac
     8521 gctgatgggg cttacatttg aaattttcgg cgccagtgtg tatttgtggc gtatgtatca
     8581 caatgtgatt caccacacct ataccaatat accgggtgcg gatcgtgata ttgatggccg
     8641 tcccttgata aaactcgcac ccagccagcc gacgtataag attcaccgtt ttcaatatat
     8701 ctacgctttt gcagtatata cgctcacatc gcttaactgg gttctgcgta aagactatca
     8761 gcgtatgttt atgaagcata acggtgtgta cacgacgaaa atgccgtcgg tgccgctcat
     8821 cgcaagcatg ttcatgttca agctcgtgca ttttacgtta tttcttattt tacccattat
     8881 tgtgttgccg gttccgtttt ggatggtgat cataggattt ttcgtgatgc acatgacgac
     8941 gggtattaca ctggcattag ttttccagat ggcgcatgtt gtagaaggaa tggagtttcc
     9001 cgaaccgcac gaagacggta tgctcgaaaa cgaatttttt gtacatcaat tgaagactac
     9061 ggctaatttt tcacgtaaaa gtaaaattgc tacatggatt tgcggcggac tcaatctgca
     9121 ggtagagcat cacttgttcc cgcatatttg tcatacgcat tacccggcga tttcggatat
     9181 cgtaaaacgt accgcgcaag agtttggaat cccgtaccac gaaaatacat cgttctggtc
     9241 ggcgctggca tcgcatgttc gccatctgcg taaagtagga tacatccctg tgcaattagg
     9301 gtaaaatttt gagataggac ttaaaaaaag tcattacttt taaaatattt ttcttatcat
     9361 tcgccgcacc aacatgcggc gaattttttg cgtgttggct cagacggatc gcataacatc
     9421 aaaagattat tattatagtt ataggagaca attaccgtaa cactatgaat acgtatctca
     9481 aaaatatagt cgagtattat cacgcgacgg aaaaagctta ccatctgcac tgggatgtga
     9541 aaaacagttt atccgtgcac tacggttatt gggacgataa agtctcttca ttttccgagt
     9601 cgctgcttcg gatgaacgaa gtgatggccg aagcggtggg aattacatca tcggataggg
     9661 tacttgacgc cggttgcggt gtaggcggga gcagtattta tcttgccaaa acccgcggct
     9721 gccgtgcggt aggaattaca cttagtgata ggcaagttgc ccaagcggag acttttgcca
     9781 aagaccgtgg tgtatccgaa cagacggaat ttaaaatcat ggattattgc catacggatt
     9841 ttccggatgc atcgtttgac gtggtatggg gatgtgaaag tatttgttat gcggaatcca
     9901 aagaacaatt tatccgcgag gcatatcgtt tgcttaaacc gggagggcgg ctggtgattg
     9961 cggatggtat gacggctaag tttgagaaca atgatcatcc gatcaatcgg cgctggctcg
    10021 agggctgggc ggctaacttt atcgaaacac cggagcgtat gcaggggtat atgcaacaca
    10081 tggggtttac ggatattcgt tttcgggata tcacaccgca tgtcatgcat tcctcgcgga
    10141 gaatgtatat gctgtacttt ccggccaagg tctatcatgc cgtagtacga atgctcgggc
    10201 gcccgtcgcc gttattattc agaaaaaatg tggatgcctg ccgtttccaa tacatcgcat
    10261 tcaaacgaaa attgtggatg tatgggcttc tgtgcggcgt taaaaaatag ctgaggagta
    10321 tcatgagtaa ttaccacgat cagatagttg actattatcg tgagacagaa aatcactacc
    10381 gtgtgtattg ggcgctggat aaaacactgg cgattcatta cggatattgg gacgaaaaag
    10441 taaaatcatt tccgcagtcc cttctgcgca tgaatgaagt gctcgccgaa accgcgaaga
    10501 tacagaaaac ggatcgtgta ctggatgccg gctgtggcgt cggcggcagc agtatttttt
    10561 tagcgcagca tatcggatgc catgcgacgg gtatcacgtt aagcgctaaa caggttgcac
    10621 gggcatttga aaatgccaaa gataaaaacg tcagcgatcg gacggatttt aaggtgatga
    10681 actattgcaa aactgatttc cccgatgcat cgtttgatgt agtctgggga tgtgaaagca
    10741 tttgttatgc ggatagtaaa gagcagtttg ttcgcgaggc gtttcgtttg ctcaaaccgg
    10801 gcgggcgatt gatattggcg gacttcatgg ttccagcttt tgaaaataat caggagccga
    10861 gcgtacgccg ttggctcgac ggttgggtcg tcaattttct cgaaacgccg gataatttta
    10921 aaaaatatat gctcgaagcc gggtttgaag atgtccggtt tcgcgatatt tccacacaca
    10981 cgcttcactc cactaaacgc ctcgggcgat tgtattattg gggtaaggtc tatctggcgt
    11041 attgcaaaat catcggcaaa acgatggacc cgattcgttt gaggaatatt aaatcggctt
    11101 acgatcaata ccaaagccat cgtcgtgcat tatggatcta cggtatgttt tgtacgacaa
    11161 aaccgtcctg attgtgctta actcgtttat ggcaggcgta ttgattaatc aaaaaactag
    11221 tttataggtt taatccatga gtgcttatca tcagaatatc atcgcatttt atgacgtact
    11281 tgaaaatcca cttcgtatga tatgggattt agacaagagt tacgcgattc actacggcta
    11341 ttgggacgaa aaagtaaagt ctttcccgga gtctcttctg cgcatgaatg aagttatggc
    11401 ggagacggcg caaatcaaat cccacgaacg cgtgttggat gccggctgcg gtgtcggtgg
    11461 gagcagcatt tttcttgccc gtacgatcgg ttgcaaaaca gtgggtatta cgttgagtgc
    11521 caatcaagta caacgcgcta agaaaaatgc gcaaaactcc ggcacagcgc atttgaacga
    11581 attcaaagtg atggattatt cgcatacgga ctttgaagat gcgagttttg atgttgtatg
    11641 gggatgtgaa agtatctgtt atgcagataa caaggaagag tttgtcaaag aggtctatcg
    11701 gattttaaag ccgggcgggc gctttatcat ggcggacggt atggtacaaa aatttgaaga
    11761 taatgataat cccatcgtgg ctaagggacg cgatggttgg gcggccaact atgtagagac
    11821 accggagcgc ttcaaacaat ttttagaaaa cacgggtttt acaaatatca cctaccgcga
    11881 tattacggat catatcatgc attcggccaa acgcctttat tgggtttcgt tcccggcgaa
    11941 attgaatcag tggattcgcc cgttgttcgg aaaaacggtg gcggaacata ataaaggaaa
    12001 tgtaaatgcg tgcaagtatc aatatttggc gttcaaaaaa aggcttttag tgtatggaat
    12061 tttgtgtggc gtaaaacgat aagctgtttt ttgtaagctt tcaaaatgaa aatttatgaa
    12121 ttcgtatcaa caaaaaatcg ttcaatatta tcacgcaacg gaaaaatcat acaaggatgg
    12181 atggggatta aaaaaaaatc tcatgatcca ttacggttat tgggatgata aagtctcttc
    12241 attttccgag tcgctgcttc ggatgaacga agtgatggcc gaagcggtgg gaattacatc
    12301 atcggatagg gtacttgacg ccggttgcgg tgtaggcggg agcagtattt atcttgccaa
    12361 aacccgcggc tgccgtgcgg taggaattac acttagtgat aggcaagttg cccaagcgga
    12421 gacttttgcc aaagaccgtg gtgtatccga acagacggaa tttaaaatca tggattattg
    12481 ccatacggat tttccggatg catcgtttga cgtggtatgg ggatgtgaaa gtatttgtta
    12541 tgcggaatcc aaagaacaat ttatccgcga ggcatatcgt ttgcttaaac cgggagggcg
    12601 gctggtgatt gcggatggta tgacggctaa gtttgagaac aatgatcatc cgatcaatcg
    12661 gcgctggctc gagggctggg cggctaactt tatcgaaaca ccggagcgta tgcaggggta
    12721 tatgcaacac atggggttta cggatattcg ttttcgggat atcacaccgc atgtcatgca
    12781 ttcctcgcgg cgtatgttat atttttattt tccgacacgc tgttatcatc tgtatcagta
    12841 cctcataaga aaaagtgtgc cggaagtgaa tcgcaaaaac attgatgcgc tgttgtatca
    12901 gtatatcgcc ttcaaaaaga aattatggct ctacggtatg ttgagcggag tgaagcatca
    12961 gtaaatattg tagtccatac aaaagaataa tcggatgatt gtatagatga aaaaaatgaa
    13021 tgatccgtaa cagaatattt ttttatgata tcggttttag atattcccat ccttccgagt
    13081 gcaaatcttc aaatgaaaat ccgtcttttt cgttgcccgg cataataaat acggcatcgg
    13141 ccatcacgta gttaaaatcc agatacggaa cggcttcgat caatttaggc caggtttcgc
    13201 ggagaaaata cgattcttcc ggtatatcta tcataccttg aaaggattct tgtgtcggta
    13261 cattttcgat taagaaggcg agcgaaataa cgacgtattg tttttctgcg ctcatgcgat
    13321 actgttcgtc catcgtttca ccgacttcgc cggactccgt tgccatactg ctttcgattt
    13381 cgttgaccca cgaatcaaat tcggcctgtg cttcggccgc atccgtcaga taatcccagt
    13441 acccatattc aaaaaacggt tcgttctcaa gtaagagggc tgcgacattt ttaaaaaatg
    13501 cttttttttc ctctggtgtt tcaccttctt taacgggcac gttgatcgcc gcatgcagct
    13561 tggcacgggt ttcatcattg ataccggtat tgatgacgat ctgaatgccg taaaaatgaa
    13621 tcggacccga gggttcatcc gacttaaaaa tattattaaa aaaaccgcga cgattcatgc
    13681 ttagtaatcc ttaaattatg atgaagaact gctgcgactg cggccaaacg atccggagcg
    13741 acctgacgaa gagcgggacg atgaactttt ggacgacgag ctccggtaac tccgcgagga
    13801 acgcgacgaa gatgacgttg aggttgagcg cgacgcacgg gatgtgccgg ttgaactgta
    13861 attgcgtgac tggcgaacgc gggtgacatt gccgtcggaa ttaacattgg cggatacacg
    13921 gccaaatccg gtcggttttt gtttgcccac gccgctggtt tgacttccga cggtacgtga
    13981 agaaaacaat gtcggacgac gttccggttt gtattccggt ttcgacggcc cttgcgaagg
    14041 cgcacgataa ccgcccgggc gactgatcgt cgaaggcgta ttatagttac caaaaaatat
    14101 cggccaatag gatggaccgc cgccgtaatg atatacggtt cgcgtggatg ccggtgttgc
    14161 gacgacagta tctttttgtg ccggtgcggt catggctttg gctatcgcgg agtcgctccc
    14221 tgtgaatgcc gcgatcatcg aatcggcgga tgttttagcc gaggccgatg tagccatggc
    14281 cgaagcgacg gctggtttga aatccgacat gggcaacaga tccacgctga tatttttttc
    14341 tttgtagtaa acaaagtcgt cattgagatc atccagttcg tttttggtgt cacgatccgg
    14401 tatgacatat aaaacccgat tgactttata cgacgcaagc gatggcgcgg aaggcacttt
    14461 ggccacgtaa cgattatcaa atacatccca ttcatcggta tattcgccca agcgatgatt
    14521 gtccaataaa atcacggccg gtgcatccca cgaaagtttt tcttccgttt tcacgcttac
    14581 ttcgcgtgca tagtacatca tcgctccgag cgtgcgatgt gccggtacga cgccttgcgg
    14641 gtgcggccag ttatcaaatc cgatgaccac attggcgacg tcggctaatg ccgcgccgaa
    14701 tgctacggat tcttccccgt gcagatcggc gatgatcatc gtggatgagg ggttgtccat
    14761 tttttgaatg atctcacgca tgccgagtcc gcgcgcatag gcggttttca tcgcgtcgga
    14821 taataccggc ttcacttgtt tacgtaacgt ttcctgactt agcgattgga ctaacgttgt
    14881 aaccttaaaa ggtttccagc gataattttt gggttcatag gccaccatca atgccgccgg
    14941 atcggcataa ttttcccacg cctgaccgag gctgtcacgg ctttgttcgt ctttgaagtc
    15001 aagattttcg ccgtccgcgc cgacctgcca gccttgtttt tgctgcaact caagagcatc
    15061 gagttcgacc tcttcaccac agcccgtaag aatggatgcg tagggcagca ttgcagcgcc
    15121 ggttgcgagg gttgccaatc gttggccggc ttcacggcgt gaaatcatgg cattccacca
    15181 tgcgggtgtt tgggcttttt gattacgttt ggtttttctt tttgccatag tttatgttct
    15241 agtgagaatg ttggtcaatc aatgtcgctg cgctgagcgc cgtaccgtcc gtggc
//
LOCUS       DAOQNQ010000047        15075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1399_length_15075_cov_6.037150, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000047 DAOQNQ010000000
VERSION     DAOQNQ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15075
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1399_length_15075_cov_6.037150"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="PK754_04925"
     CDS             complement(<1..984)
                     /locus_tag="PK754_04925"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNB08877.1"
                     /translation="MKKVLFLLLNVLVSVFFVWLATRGIDWTQLGSSLTSISLTLVIF
                     SVIINQFSLWLRAWRWKFLLAPLKDVPTLSLFSNVSIGFMINNILPFRLGEIARGYIL
                     KKEHQISFTASMATVVVERVIDVISLLTVFGVLVIIFPFPEWIKTAGMVVGAIDLAAI
                     IFMILLVKNTEATCRLVQSMISVFPDSLKQRVDRLLRSFVSGITFTHALGTTVVIAML
                     SFLIWAVYTVSIWIMFKACNAPELATLDLIDATIVMTFSSFAVMIPAAPGYVGTFHEV
                     VKQSLMMFRVDREVALGFAIVIHAFNYTAQIVIGVGYWIKTNFNLKEALSNV"
     gene            complement(997..1632)
                     /locus_tag="PK754_04930"
     CDS             complement(997..1632)
                     /locus_tag="PK754_04930"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNB08878.1"
                     /translation="MKYLIVSLMALSGVWAQGKPEDAQSVIRKVQNKYKKTDSFSFSF
                     DYRFKWKLTGKSQSAQGKLFFKKENNIRYELGQQLNITNGESVWQYSEVNNQVIIDKL
                     KKNSRSLFMPKYFLYDYLEKFIAEVIKTETINGRLVYVLKLDPRDRDDFVQSMKIWVP
                     SDTWITQKIEYADLEGNQVTYEFTHVEIDQPMNMNLFTFTPETGMDVIDLR"
     gene            complement(1689..4850)
                     /locus_tag="PK754_04935"
     CDS             complement(1689..4850)
                     /locus_tag="PK754_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNB08879.1"
                     /translation="MASKKKNNPKPKAAESPKGKIPQKPQPPKGLKLNLGIKLKEEIF
                     GLLLMTVSLLSILSVVTHVANEQPGTIMELWRAGQLQNLLGPVGAMLSHYLVGYTFGY
                     PFVAVPILFLVYGWLFFTHKSIFLVNQFAFYILTLMLIVSTWLAIPGALDHSENWNAS
                     GMLGGMLAHQLYTWLGGFGSVTIWVVFSLIWITMVTRVSIADLIPAIKNILFTSYRTT
                     TGTISKFKSRQQELLEKENEVSDDELDKVNEDATEKPLIPEYLQKKTIKTSVSPTSAK
                     TEKVNEPVKTEIPQVTTKQSTTIAPAIKEEKAPEESVAVQAKAEEPKREAITGTDLGM
                     DTQFVRDLKAIGHIDKKFSINRTKLEELSASAGHTDSHQDLPQEETTAPANTPVTENP
                     KPRVVEPAKDLTIRPEEADLDMADADFKDLARSMFDAEHQHIEWEKEWVKEETAELER
                     AKTRLDMKPARKLKTQTVKIEDIVETEEAPKEDIHNIVPESTDASQLSTNDINPVTQE
                     EPQPEVPLPETAQEAEALAASIKAVKKEAPKPEPAIDFDKENSQARKKYKFPSIDLLE
                     PTQSLEKLTPEEIGYLNTKVEQIIAKLAEFGIATKVVATEYGGPVVAIYQLELPSGLK
                     ISKITGLEDELALSLKVKSVRMVPVTSKGTIQVEIPKLKAEPVMIRSLFEDPSFRDAK
                     NKYKLGLALGKTIDGHIHFEDLAKMPHLLIAGTTGAGKSVGINSIITSLLYQFDPSEV
                     KFIMVDPKKVELALYRRLKNHHLISLRNHRGEVIEDVITKPENAKLILKALVEEMEER
                     YEKLAEANVRNLEDYNKRWKEGRMPHDGKYQHYKLEYIVAIIDELADLMMTAPREIET
                     SITRLAQMARAVGIHLIVATQRPSVDVLTGLIKANFPARIAYAVRSKIDSRTILDMGG
                     AEQLLGKGDMLFMPPGQVPIRIQNAFTSTQETETIVDFINRMQAFPRRDFMVKEEPRE
                     ENEGGEVDSSGFDELFNQAMEIVVKHNQGSASLLQRRLSVGYARAARLIDQLERAGIV
                     SAPDGAKPRQVLITEDQMAMYMN"
     gene            complement(4894..6009)
                     /gene="gcvT"
                     /locus_tag="PK754_04940"
     CDS             complement(4894..6009)
                     /gene="gcvT"
                     /locus_tag="PK754_04940"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNB08880.1"
                     /translation="MSTSTNPQKTPFYDKHIAAGAKMADFAGYYMPIRYDGDIVEHQR
                     VRTAVGVFDVSHMAEFMVTGEGAADFVQKITVNDVSKMADTQAQYSAMCKPDGGIVDD
                     LIVYKYNDKKFMLVVNASNMEKDFNWVKSHCPSHLQLENISDDIALLAIQGPHAKEVV
                     QSLTKTNLSDIKFYWFREGEVDGVPMTISRTGYTGEKGFELYFNTKFADQIWNAVMKA
                     GEKYNIGLAGLGARDTLRLEMNYRLYGNDIDETTNPLEASLGWITKLNKGEFIGREAL
                     QKIKADGVKRKLVGLEVEGKIPARHGYDIMSDGRKVGVVTSGTFSPSLQRAIAVGYVE
                     TALAKDGTMLKINARGRELNAKVSPTPFYNGDPSSDF"
     gene            complement(6097..7275)
                     /locus_tag="PK754_04945"
     CDS             complement(6097..7275)
                     /locus_tag="PK754_04945"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNB08881.1"
                     /translation="MSDFKVAPASAMSRLGTETAFEVLVKAKALEAQGKHIVHLEIGE
                     PDFDTPTNISEAAIKALREGWTHYGPSPGLPDLRKAIADYVSKTRHIETHPDEVVVTP
                     GGKPIIFFPIMALIDPGDEVIYPNPGFPIYESMINFVGGKAVPLHISEEKNFTFDVSE
                     LKEKISHKTKLVIINSPANPTGGVFSKKDIEDIAAIVKQYNCYVMSDEIYSRIMYGYE
                     HYSIASLPGMKERTIILDGFSKTYAMTGWRAGFGVMPKDLAAMVSKLQTNSNSCTNSF
                     VQKACIEALQGPQDSVNAMVTEFEKRRDFIVDGLNAIKGFSCKKPLGAFYVFPNITKT
                     GLDSRKLADYLLNEAGVAALSGTAFGSFGEGYLRFSYANSLDNIKLALERIDKAMKKL
                     "
     gene            complement(7534..9504)
                     /locus_tag="PK754_04950"
     CDS             complement(7534..9504)
                     /locus_tag="PK754_04950"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HNB08882.1"
                     /translation="MVIIFFVLTAMALQNCDEHRSRNSSEDDFFGHTLDTTFAHAVTI
                     AYSDSSISVINPFDTLGVTISIDGGDVVVNSTITGTEIQYVLSGKISDGMFKIYSVYK
                     LALVFNGVSITNDNGPAVNVQSSKKVSVNIVYGTNNRLIDGASYITTSEDQKATLFSE
                     GQLSFSGTGSLKVKGNYKHGIAGDDYIYIKESNIDIIAAASDGVHANDYFTMESGYLH
                     VVASGDGIECEEGFIAILGGHITVNSIDEGITASYEDGDISVTPYIQITGGTIHVTTS
                     GAKAHAIKSESHTTINSGETIMLTVLGPGAKGIKTGGNLTLINNSITITTGGNAYYDA
                     SASDIVSSAGIDCDGYFLMEQGTLTVTSSGTAGKGINIDSTMEMNNGTISVSTTGAGY
                     IYGSLTSEAKAIKCDGAITINDGTIMVSSADDGIKSETALTINGGTVTVTKSYEGLEA
                     PLVTMNNGNVSVTASDDAVNTTYGNGGEAVDGSLLTFAGGTIALNSSNGDAIDANGNI
                     EMTGGVVIAQGPKVYPEVAIDCNGNFNISGGLLIASGPNAGPMIEATSTTSTQYTALI
                     KIISNVSPGRLFNIQDSEGNSLVTYAPSRVAYYFVFSSPLLQNGATYKVFTGGNCTGA
                     TVTNGLYFGGTYSGGTQKGSFTISSKVTTATL"
     gene            9779..12109
                     /locus_tag="PK754_04955"
     CDS             9779..12109
                     /locus_tag="PK754_04955"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HNB08883.1"
                     /translation="MADPKSFEALEQRLNHITQTLIAAQQEIGRLRSEIQTLRAESHP
                     ASTPLTVEEKSHTVLPTPVTTAYTIAKPAVSEIPKSKISAEEPKKNIEAFIGGKLLTI
                     IGIVVLVIGLGILVIAAIEADMIGPIGRVMIGILSGLGLLVVAKKLKRTYELFSAALL
                     SGGMATLYFSVFAAFSFYALIPQWAAFVMMLMLTIFTVLAATRYDRQIIGVMGLVGAY
                     GVPFLVSTQSGNILVLLSYITLVNAGILILGFRKNWRIMNHTAFVLTWAIFTAWLVDR
                     YDEKTQRPIALGFSTLFYIKFYLLFIAYKIRSKEPFAAYDIIRLLGNSFVYYGVGYGI
                     LDSVSHGAYQGLFTMGIAATHIAFGYYLRKTQNGERALVLAVAGMTLIFTAIAIVVQL
                     EGHWVTMLWLAVASVVFWMGRSQNEVWYVRMAVALYIIGFISFIHDQQHVVLVNTDLV
                     VVWKPVFNLNFLSALWAIGAFAFSQYIIFHPSYVTKNAPPSQIDRIGLMIISGLTALT
                     VFFAFAYEIHFYFQSLYRQTYTDWHDIARADISFLYYNTAWISLYTGLFLYAFTVLIC
                     RFYKDWKGFIAASVLAGFYLILVLTQTVDAMGELRSFYIAPPGEYPFVVGFSSMLIRF
                     GVWSAVGLLLYGISSYAKAAEPKFSEPLHVIINICVIIILSLELKNAWLVTHADDTSL
                     LATQVYRIGFTILFGIYSLTLIAIGIWKRHRMMRYFAIALFALTLVKIYTYDMQNLSS
                     FNRILLFIPLGLILLLVSFLYQKFKHIITGDEKTNA"
     gene            12102..13322
                     /locus_tag="PK754_04960"
     CDS             12102..13322
                     /locus_tag="PK754_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 family protein"
                     /protein_id="HNB08884.1"
                     /translation="MRRLFLFLLIVSGTTAHGQFSSYRMAADLDSVRQNGWHHLHILP
                     SWLALMREDKADVRLFSFRKNDTLETPYLMRKSEDRMRTKEIPVHVINRGLSGRTLFL
                     TLSQPSKKIINSLTFSPEAINFNCGVLIEGSDDQKNWIRLATDEPQRIFGIQNSDISY
                     RYTTLRFTETNFRYLRLQLSDASQLGTVHVEATENTVDWAELDTLRITSTTTTENHTA
                     HTTEMIMEFENPSAVDRLQLRFSNTGDFYRSVEVLARGDSIGGRNGHNAWWYPVSSGV
                     VSSLEPAILRNLSVRTSAIKLVIHNHNDQPLKITNAMVFAERTYLAAKLEKNTRYVLT
                     FGKKNDQAPRYDVVYFQETIPSVLPIISAGANFSLQSENNVTTFGQNWWIWVLLIVAA
                     VILGWMAMGLIKEK"
     gene            complement(13333..>15075)
                     /locus_tag="PK754_04965"
     CDS             complement(13333..>15075)
                     /locus_tag="PK754_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB08885.1"
                     /translation="SGGQIQVLVDDMKIFGACIDKMDPVTSYVEIDNLEKIEVAKGGF
                     DMSAGQTIGGSLNLRTGKPDFSVPFAIGVQTGYENVSHLRFARSTTNWSGGAWAARAG
                     VSLRKSDDFRAGDGRLMHNSGYNKSNIKTDIAHQNGNHTWTASYIRDVATDAGYPVLL
                     MDTRRAVANIFGLEHQWRQPWHHASFLNTKVYFNMVDHDMDDYDRDVTARDFMPNMYM
                     PMYGNTRTIGFSSSLYTGGNIQGIKWVVDFYRMTAFADMDMQSLDPEVSPMYLVNIGS
                     THIYNYAVAAEYRRLLTEKIVFKSSFRLDYSVRSLHDDIGRRELEGYWNRTHLNTSYI
                     TFSASAAAEWQMMADQKLRLALARTARTPTHIENYGFYFYNVSDGFIYTGNPNLKPEV
                     SYQSELSYEYKTDKHISKAQIFYTRIDRYIAGITTDTLFKQIENIHHADLAGIEASHF
                     RSITNTIAWQITASYRYSRVSAWDEPMPGMAPLEVLNSVRWVPGSYWFELSNKTAASQ
                     NRFAKQQAVEDRTHGFTLFTIRGGIKVNKHAELQWGVENVLDTDYREHANIQKMPGRG
                     RNVYLTLQWNHWAK"
BASE COUNT         3925 a         3607 c         3189 g         4354 t
ORIGIN      
        1 tacattcgat aacgcctctt tgagattaaa attagtttta atccaatatc cgacaccaat
       61 gactatctgc gcggtataat tgaaagcatg tatgacgatg gcaaatccaa gcgcaacctc
      121 ccgatcaaca cgaaacatca taaggctctg cttgacgact tcatgaaatg tgcccacata
      181 acccggcgcc gccggaatca tcacggcaaa cgaagaaaaa gtcatcacaa tagtcgcatc
      241 tataagatcc agtgtcgcca actcaggggc attgcatgct ttaaacatta tccatattga
      301 tacggtataa acagcccaga tcaaaaaact gagcatagca atgactacgg ttgtacccaa
      361 agcatgcgta aatgtaattc ccgacacaaa agatcgtaaa agacgatcta cgcgttgctt
      421 gaggctatcc ggaaaaactg aaatcattga ttgcaccaag cggcatgtgg cttccgtatt
      481 ttttacaagc aggatcatga aaataatagc cgctaaatct atcgcgccga caaccatgcc
      541 ggccgttttg atccattccg gaaaaggaaa aataattacc agtacgccaa aaaccgtcag
      601 caacgaaatc acatcaatga cgcgttccac caccactgtt gccatcgagg ccgtaaagga
      661 aatctgatgt tcttttttta gaatataacc tcgcgctatt tctccaaggc ggaacggcaa
      721 aatgttattg atcataaatc cgatactgac atttgaaaac agactaagcg tcggcacgtc
      781 ttttaaggga gccaataaaa acttccatcg ccaggcacga agccataagc taaattgatt
      841 gatgattact gaaaagatca caagcgtaag actgatcgaa gttaatgagg aacccagttg
      901 tgtccaatct ataccgcgtg tcgcaagcca tacaaaaaaa acactaacca aaacattaag
      961 cagaagaaat agtacttttt tcaagttaca ctccggtcat cgcaaatcaa tcacatccat
     1021 accggtttcc ggcgtgaatg taaaaagatt catattcatc ggctgatcta tttcgacgtg
     1081 ggtaaattcg tatgtaactt gattaccctc cagatcggcg tattctattt tttgggtgat
     1141 ccacgtatcg gatggcaccc atatcttcat gctttgcaca aaatcatcac ggtcccgcgg
     1201 atcaagtttg agcacataaa ctaatcggcc gttgatcgtt tcggttttaa tgacttcggc
     1261 aataaatttt tccaaataat catacaaaaa atatttgggc ataaacaggc tgcggctgtt
     1321 ctttttcagt ttatcaataa tcacctgatt attgacttcc gaatattgcc acaccgattc
     1381 cccgttggtg atattgagtt gttgacccag ctcatagcga atattgtttt cctttttgaa
     1441 gaacaatttg ccctgagcgc tctgactttt tccggtcagt ttccatttga aacggtaatc
     1501 aaacgaaaac gaaaaactgt ccgttttttt atacttgttt tgcactttac ggatgaccga
     1561 ctgtgcgtct tccggtttac cttgagccca aacaccggaa agagccatca aacttacgat
     1621 caaatacttc aaacgataaa ctccattctt tggaaaatgg ttatacaata aacataatca
     1681 aacgaacact aattcatata cattgccatc tgatcttccg taataagcac ctggcgtggt
     1741 ttggccccgt cgggagcgct tacgataccc gcacgttcca gttgatcgat aagccttgcc
     1801 gcgcgagcat aaccgacaga taggcggcgt tgtaaaagcg aagctgaacc ttgattatgt
     1861 ttgacgacga tctccatcgc ctgattaaag agttcatcaa atcccgacga atccacctca
     1921 ccgccttcgt tctcctcacg cggttcttct ttcaccataa aatcacgacg cggaaatgcc
     1981 tgcatgcggt ttataaaatc aacgatggtc tctgtttcct gcgtcgaagt aaacgcgttt
     2041 tgaatacgta tcggcacttg cccgggcggc ataaaaagca tgtcgccttt gccgagtaac
     2101 tgctccgcgc cgcccatatc caggatggtt cgcgaatcta tcttggagcg gaccgcgtaa
     2161 gctatgcgcg ccggaaaatt ggctttgatc aagccggtca aaacatctac cgaaggacgt
     2221 tgtgtggcga caatcaggtg aataccgaca gcacgcgcca tctgtgcaag gcgcgttatg
     2281 gaagtttcta tctcgcgcgg tgccgtcatc atgagatcgg caagctcatc gatgatcgca
     2341 acgatgtatt ccaatttgta atgctgatat ttgccatcgt gcggcatccg gccttctttc
     2401 caacgcttat tataatcttc caaattacgt acattggcct ctgccaattt ttcataacgc
     2461 tcttccattt cttcgacaag cgctttgaga ataagctttg cgttttccgg tttcgtaatg
     2521 acatcttcaa taacttcacc gcgatgatta cgcagggaaa tcaaatgatg attttttaac
     2581 ctgcgatata gcgccaattc gaccttcttg ggatccacca tgataaactt cacttccgac
     2641 ggatcgaact gatacagaag actggtgatg atcgaattga tacctaccga tttaccggcg
     2701 ccggtcgtac cggcgatcag caagtgcggc attttggcga gatcttcgaa atgaatatga
     2761 ccgtctatcg ttttccccaa tgccaaaccc agtttgtatt tgtttttcgc atcgcgaaat
     2821 gacggatctt caaaaagact gcgaatcatc acaggctcgg ctttgagttt cggtatttcg
     2881 acctgtatcg tgcctttgct ggtaaccggt accatacgta cggatttgac tttgagggac
     2941 aatgcaagtt cgtcttccaa gcccgtgatt ttggaaattt ttaatcccga cggtaattcc
     3001 agttgataaa tagctaccac cggtccaccg tattccgtag ccaccacttt ggttgcaatg
     3061 ccaaactcgg ccagcttggc aatgatctgt tcaacttttg tattaaggta gccgatttct
     3121 tccggagtca atttttcaag tgactgcgta ggctccagca aatcaataga aggaaactta
     3181 tatttttttc gcgcttgtga attttcttta tcaaaatcaa tcgcgggttc gggtttcggc
     3241 gcttcctttt ttacggcttt gattgacgcc gccaaagcct ccgcttcttg tgctgtttcg
     3301 ggaaggggta cttccggttg cggctcttcc tgcgttacag ggttaatatc gttcgttgat
     3361 agttgtgatg catccgtact ctccggcacg atattgtgaa tatcttcttt tggcgcttct
     3421 tcggtttcga cgatatcttc gatttttacc gtttgtgttt tcagttttcg cgccggcttc
     3481 atatcaaggc gcgtttttgc gcgctcaagt tcggctgttt cttctttgac ccattctttc
     3541 tcccactcaa tatgctggtg ctccgcatca aacatactgc gggcaagatc tttgaagtcg
     3601 gcatccgcca tatcaagatc ggcctcttcc ggtcttatcg tcaaatcttt tgccggttcg
     3661 accacacgcg gcttcggatt ctcagtcaca ggtgtatttg cgggcgccgt cgtttcttcc
     3721 tgaggtaaat cctgatggga atcggtatga cctgcggatg cggagagttc ttccagcttg
     3781 gtgcgattga tcgagaattt tttatcaata tgtccgatcg ctttgagatc acggacaaat
     3841 tgcgtatcca ttcctaaatc tgttcccgtg attgcttcgc gtttaggctc ctccgcttta
     3901 gcctgcacgg cgaccgattc ttccggcgct ttttcttcct tgatcgccgg ggcaatcgtc
     3961 gtactttgtt tcgttgtgac ttgaggaatt tccgttttta ccggttcgtt aactttttca
     4021 gtttttgcag acgtcgggga tactgacgtt ttaatggttt ttttctgcaa atactcaggg
     4081 attagcggtt tttcggtagc atcttcgttg accttgtcaa gttcatcgtc tgaaacttca
     4141 ttttcttttt ccaataactc ctgttggcgg gatttgaatt tggaaatcgt tccggtcgtc
     4201 gtgcgatacg atgtgaaaag aatatttttg atcgctggga taagatcggc aatactcacg
     4261 cgggtaacca tcgtgatcca gatgagcgaa aaaacaaccc agatcgtcac gctgccaaag
     4321 ccaccaagcc acgtgtacag ctgatgcgcg agcataccgc ccaacatacc gctcgcattc
     4381 cagttttcgg aatgatccaa cgccccaggt atcgccagcc acgttgaaac aatcaacatc
     4441 agcgtgagaa tgtaaaacgc aaactgattg acgagaaaaa tacttttgtg cgtaaagaag
     4501 agccaaccgt ataccaaaaa taaaatcggc acggcaacaa aggggtaacc gaaggtataa
     4561 cctaccaaat aatgagataa catcgcaccg accggaccga gcaggttctg caattgccca
     4621 gccctccaaa gttccatgat cgtgccgggt tgttcattag caacatgcgt cacaaccgac
     4681 aaaatagaca ataaactcac ggtcatcagc aacaaaccaa agatttcttc tttgagtttg
     4741 atgccaagat tgagtttcag cccctttggc ggctgcggct tttgaggtat cttaccttta
     4801 ggcgattcgg ccgctttggg tttgggatta ttttttttct ttgacgccat tcaaatgtta
     4861 ttagaattca tatttaacca agacgccttg tctctagaaa tccgaactgg gatccccgtt
     4921 ataaaacggt gtcggtgata ctttagcgtt aagctcacgg ccgcgtgcat tgattttgag
     4981 catcgtaccg tcttttgcca atgccgtctc tacgtacccc acggcaatcg cgcgttgtaa
     5041 actcggcgaa aatgtgccgc tggtcactac gccgactttt cgtccgtccg acatgatgtc
     5101 gtaaccgtga cgcgccggta tttttccttc gacttcaagg ccgaccagtt tcctcttaac
     5161 gccatcggct tttatttttt gtaatgcttc gcgcccgata aactcgcctt tattcagttt
     5221 ggtgatccag ccgagcgaag cctcaagcgg gtttgtcgtt tcatcaatat cgttgccata
     5281 caagcggtaa ttcatctcca aacgtagcgt atcccgcgca ccaagaccgg ctaaaccgat
     5341 attgtatttt tcaccggctt tcatcaccgc attccatatc tgatcggcaa acttcgtatt
     5401 gaaatacagt tcaaaacctt tctctccggt atatccggta cgtgaaatcg tcatcggcac
     5461 gccatccact tcgccctcgc ggaaccaata aaatttgatg tccgacagat tggtttttgt
     5521 cagcgactgt acgacttctt ttgcgtgggg tccctgaatc gcaagcaacg caatatcgtc
     5581 gctgatattt tccaattgga gatgagaagg gcaatgcgat tttacccaat taaaatcttt
     5641 ttccatattg gatgcgttga cgacaagcat aaatttttta tcgttgtatt tatacacgat
     5701 cagatcgtcc acaataccgc cgtcgggttt gcacatcgcg gaatactgtg cctgcgtatc
     5761 ggccatttta gaaacatcat tgaccgtaat tttttgtaca aaatctgccg cgccttcacc
     5821 ggttaccata aattcggcca tgtgtgacac atcaaataca ccgacggctg ttcgcacgcg
     5881 ttgatgttcg acgatatcgc cgtcataacg aatcggcatg taatagcctg caaaatcggc
     5941 cattttagca ccagctgcga tatgtttatc gtagaacggt gttttttggg ggttagttga
     6001 agtactcaaa atatgttctc caaattcttt actacttaaa aatgacttat ttactatatc
     6061 agtcaattca caaaagacaa tctactttca attttcttac aatttcttca tcgctttatc
     6121 aattcgttcc agtgccaatt tgatattatc gagcgaattg gcataagaaa accggagata
     6181 cccttcaccg aaactgccga atgctgtccc ggataaagct gcgacaccgg cttcattgag
     6241 cagataatcc gcgagcttac gcgaatccaa accggttttg gtgatattgg gaaatacata
     6301 aaacgcaccc aacggttttt tgcaggaaaa acccttgatc gcattgagtc cgtccacgat
     6361 aaaatcgcgg cgtttttcaa attcggtgac cattgcattc acgctgtctt gcggcccctg
     6421 caacgcttcg atgcatgctt tttgtacaaa actattcgta cacgaattac tgttggtttg
     6481 cagtttggaa accatcgctg cgagatcttt gggcatcaca ccaaaaccgg cgcgccatcc
     6541 ggtcatcgca tacgttttgg aaaatccatc caaaataatc gtgcgttctt tcatgcccgg
     6601 aagactggct atggaataat gttcgtaccc gtacatgatg cgtgaataaa tttcatcgga
     6661 catcacgtaa caattatatt gtttgacgat cgccgctata tcttcgatat cttttttgct
     6721 aaacaccccg ccggttggat tggcgggcga attgataatc acaagtttgg ttttatgcga
     6781 aattttttct ttcagttcgc tcacatcaaa ggtaaaattt ttttcttcgc tgatgtgcag
     6841 cggtacggct ttgccgccga caaaattgat catggattca taaatcggaa aaccgggatt
     6901 aggataaatc acttcatcac ccgggtcaat caaggccatg atgggaaaaa agatgatcgg
     6961 tttaccaccg ggcgtgacga ccacttcgtc cggatgggtt tcgatatgac gggttttgga
     7021 aacataatcg gcaatcgctt tgcgcaaatc gggcaaaccg ggtgacggcc cataatgcgt
     7081 ccatccttca cgcagcgctt ttatcgccgc ttcggaaata ttggtcggcg tgtcaaaatc
     7141 cggttcgccg atttccaaat gtacgatgtg ttttccttgc gcctccaaag ccttggcttt
     7201 tacaagcact tcaaaagccg tttcggttcc caaacgactc atggccgaag ccggagcaac
     7261 tttaaaatca gacatgccca aaactctccc gtgttattaa agctaaatct ctaataaaga
     7321 agaatttacc gtttttaaat ggttttatca agaaattatt cccatgcaaa tccgcacaca
     7381 aaaataaata tcaccgtgct ttatttaacg tgtaactcgt acttattttt aagtcgtcta
     7441 tcacatcaca taaaaaaacc tccgtttcat aaagcaaagg ttgatgataa tatgattttt
     7501 gttatggtgc tggccgatac gattaaatgc aatttataat gttgctgtag ttactttaga
     7561 tgagattgta aagctgcctt tttgcgttcc accggaataa gtgccgccga aatacaatcc
     7621 atttgtcacg gttgctccgg tacaattacc gccggtaaaa actttgtagg tcgctccatt
     7681 ttgaagaagc ggagaggaaa atacgaagta ataggcaact cttgagggcg cgtaggtgac
     7741 caaactattt ccttcggaat cctgtatatt gaataaacgg cccggcgata cattgctgat
     7801 tatttttatc aaagccgtgt attgcgtaga tgtcgtactc gtcgcttcga tcattggtcc
     7861 ggcattaggc cccgatgcaa tgagcaaacc tccggaaatg ttaaaatttc cgttacaatc
     7921 aatagccact tccggatata ccttcggccc ttgcgcgatc accacgccgc cggtcatttc
     7981 tatgttgcca ttggcatcga tcgcatcgcc gttcgatgaa ttcagcgcta tcgtgcctcc
     8041 ggcaaaagtt aacaaactgc cgtcaaccgc ttcgccgccg tttccatagg tcgtattgac
     8101 cgcgtcatcc gaagctgtaa ccgaaacatt tccgttattc atcgtcacca aaggcgcttc
     8161 taaaccttca taggattttg tcaccgtaac tgttccgcca ttgatggtca gcgccgtttc
     8221 ggattttatc ccgtcatcgg cggatgaaac catgatagta ccgtcattga tggttattgc
     8281 gccatcgcat tttatcgctt tggcttcgct cgtcaacgat ccatagatat agccggcacc
     8341 ggtagtgcta acgctgatcg tcccgttatt catctccatc gtgctgtcaa tatttatacc
     8401 tttacccgcc gtaccggaac tggtaacggt aagagttccc tgttccatca gaaaatagcc
     8461 gtcgcaatca atccccgctg aggaaacaat atccgaagcc gatgcatcat aataagcatt
     8521 gccgccggtc gtgatcgtga tactattgtt gatcaatgta agattgccgc cggtcttgat
     8581 gcctttggcg cccggaccta aaaccgtgag cataatggtt tcgccgctat taatagtagt
     8641 atggctttcg ctttttattg catgtgcttt agcgccggat gtggtgacat ggatcgtacc
     8701 gccggtgatt tgtatgtacg gtgtaacgga tatatctcca tcttcgtacg acgcagtgat
     8761 accttcgtct atggaattga ccgtgatatg tccgccaagg atggcaataa atccttcttc
     8821 gcattcgatg ccgtcaccgg aggcgacaac atggagataa ccgctctcca tagtgaaata
     8881 atcattggcg tgcacaccat cggaagccgc ggctataata tcaatgttgc tttctttgat
     8941 ataaatataa tcatcaccgg caatgccgtg tttgtaattc ccttttactt tcagtgaacc
     9001 ggtaccgcta aaactaagct ggccttcgct gaatagtgtt gctttctgat cctcggacgt
     9061 cgtgatataa gatgcaccgt ctatcaatcg gttattggta ccataaacaa tgttgaccga
     9121 aactttttta ctcgattgca cattcacagc cggtccatta tcattagtga tacttacccc
     9181 gttgaaaacc aaagccaatt tgtaaacgct gtatattttg aacattccgt cggaaatttt
     9241 tccggataag acatactgga tttccgttcc cgtgatcgtg gaattcacta ccacgtcgcc
     9301 gccgtctata gaaattgtga cacctaacgt atcaaacgga ttaatgaccg aaatcgaaga
     9361 gtcggaatac gctatcgtaa cggcatgagc aaaagtggta tccaatgtat gcccgaaaaa
     9421 atcatcttcg ctgctgttac gactacgatg ctcatcacaa ttttgtaatg ccattgctgt
     9481 cagaacaaaa aaaatgatta ccaaaaacct ccgcatgcgt accaccgatt tttcttttaa
     9541 tgtgttaaag aaaagaatat ttgaatcacc cgtgttcatc gttcagatgc ccggtaagtt
     9601 aagaagttta attttttgcg aatactctgg ccgcttcgtc ccgtcggcaa tgtcttaagc
     9661 ttaacacaga tttccaaata aaataaacac tgacattgct ccttcacata actaaatatt
     9721 gaggcgatgt aatttttttt gtacgttgga tgattcacat ccaatccgga ataccatcat
     9781 ggctgatccc aaatcttttg aagcgttaga acaacggctc aatcatatca cgcaaacact
     9841 tatcgcagcc caacaagaaa tcgggcgact gcgcagtgaa atacaaacat tacgagcaga
     9901 aagccatccc gcgtccacac cacttacagt tgaagaaaaa tcacataccg ttttaccaac
     9961 tccggttaca acggcttata cgatcgccaa accggccgtc tcggaaatac ccaaatccaa
    10021 aatcagcgcc gaagaaccta aaaagaatat cgaagctttt atcggcggca aacttctgac
    10081 gatcatcggt attgtcgtat tggttatcgg attgggcata ttggtgatcg ccgccataga
    10141 agctgacatg atcggaccca tcggacgcgt catgatcggg attttgtccg gactcggttt
    10201 attggtcgtc gcaaaaaaac tcaaacgcac gtacgaatta ttttctgcgg ctttgttgag
    10261 cggcggcatg gcaacgcttt attttagcgt gtttgcagcc ttttcatttt acgcactgat
    10321 tcctcaatgg gcggcttttg tgatgatgct tatgctcacg atttttaccg tgctggcggc
    10381 tacccgctat gatcgccaga tcatcggcgt gatgggtctt gtcggcgcgt acggcgttcc
    10441 ttttttggta agcacccaaa gcggaaatat actcgtattg ctctcctaca ttactttggt
    10501 caatgccggt attttgatac tcggttttcg taaaaactgg cgcatcatga accatacggc
    10561 atttgttttg acatgggcta tttttacggc gtggttggtt gatcgttatg atgaaaaaac
    10621 acaacgccct atcgcgttgg gattcagcac attattttat atcaaatttt atttactctt
    10681 tattgcatac aaaatccgct ccaaagaacc cttcgcggcg tatgatatca tccgtctgct
    10741 gggaaatagt tttgtttatt acggcgtcgg atacggtatt ttggattcgg tatcacacgg
    10801 cgcttatcag gggttattta cgatgggcat cgcggcgaca catatcgcat ttggttatta
    10861 tttacgaaaa acccaaaacg gagagcgtgc tttggtgctc gccgtagcag gtatgacgct
    10921 tatttttacg gctatagcta ttgtcgttca gttagaagga cactgggtaa ccatgctctg
    10981 gctcgccgtc gcatctgttg ttttttggat gggtcgttcc caaaacgagg tctggtatgt
    11041 acgcatggct gtggcactat atatcatagg attcattagt tttattcatg accagcagca
    11101 tgtggttttg gttaataccg atcttgtcgt cgtatggaaa cctgttttca atctcaattt
    11161 cctgagtgca ctgtgggcca tcggcgcgtt tgctttttcc caatacatta tttttcatcc
    11221 gtcatacgtc accaagaatg ctcccccatc ccaaatagat cgcatcggtt tgatgataat
    11281 ttcaggttta acagcgctga ctgtgttttt tgcttttgcg tatgaaattc atttttattt
    11341 ccaatcgcta taccgacaaa cttacaccga ttggcatgat atcgcccgtg ccgacatttc
    11401 gttcctatat tacaatacgg catggattag tttatacacc ggattatttt tatatgcgtt
    11461 cacggttttg atctgtcgtt tttataagga ctggaaaggc tttatcgcgg cgtccgtttt
    11521 agcgggcttt tatcttatcc ttgtgttaac acagacggta gacgccatgg gcgaactgag
    11581 aagcttttat attgctccgc ccggtgaata tccttttgtc gtcgggttct cttcgatgct
    11641 tatccggttt ggcgtctggt ctgccgttgg attactttta tacggaatat catcctatgc
    11701 caaagcggca gaacccaagt ttagcgaacc tttgcatgtc atcatcaaca tctgcgtgat
    11761 tatcatcttg tccctcgaat taaaaaatgc ctggcttgta actcatgcgg atgatacttc
    11821 actgcttgca acgcaagtat atcgcatcgg atttaccata ttattcggca tctactctct
    11881 gacgttgatc gctatcggta tatggaaacg ccaccgtatg atgcgttatt tcgcgatcgc
    11941 tttatttgca ctcacgctcg tcaaaattta tacctacgac atgcaaaacc tcagttcgtt
    12001 taaccgaatt cttcttttta ttcctctagg gcttatcttg ttattggttt ccttcttata
    12061 tcaaaaattc aaacacatta tcacaggcga tgaaaaaaca aatgcgtaga ttgttccttt
    12121 ttttattaat cgtgtccggc actacagcgc acggacaatt ttcctcctac cgcatggccg
    12181 ctgatttgga ctccgttcgg caaaacggct ggcatcattt gcacatttta ccttcgtggt
    12241 tagcgctgat gcgcgaggac aaagctgacg taaggttgtt ttcttttcgc aaaaatgata
    12301 cgctggagac accgtacttg atgcgcaaaa gtgaagatcg tatgcgtacc aaagaaattc
    12361 cggttcacgt tatcaatcgc ggtctttcag gccgtacact ttttctcacg ctgtcacaac
    12421 cctcaaaaaa gatcatcaat tctctgacgt tttcaccgga agcaattaat ttcaactgtg
    12481 gcgttctcat cgaaggcagc gatgatcaaa aaaattggat acgactcgcg acggatgaac
    12541 cgcaacgtat tttcggaatt caaaattcag atatttccta tcgctatacc acacttcgtt
    12601 ttacagaaac caattttcgg tatttacgat tacaactgag tgacgcgagc caactcggta
    12661 cggtccatgt tgaagctacc gagaataccg tggattgggc cgaattggac accttgcgca
    12721 taacctcgac cacgacaaca gaaaaccata ccgcccatac gacggaaatg attatggaat
    12781 ttgaaaatcc atcggcggta gatcgcttac aattacgttt ttccaacacg ggagactttt
    12841 atcggtcggt ggaagtactg gcgcgcggcg acagcatcgg cggtcgtaac ggacacaatg
    12901 cctggtggta tcctgtttca tccggtgtag taagttcttt ggaaccggcc atcctgcgca
    12961 acttgtcagt acgcacttcc gcgataaagc ttgtcatcca caaccacaac gatcaacctt
    13021 tgaaaattac gaacgcgatg gtattcgccg aacgaactta cttagccgct aagttggaga
    13081 aaaacacacg ctatgttttg accttcggaa aaaaaaatga tcaagccccg cgttatgatg
    13141 tcgtttattt tcaggaaacg ataccttcgg ttttaccgat aatttctgcc ggtgcaaatt
    13201 ttagcctgca aagtgagaac aacgtaacta cgtttggtca aaactggtgg atatgggtat
    13261 tacttatcgt agccgcagta atactcggat ggatggctat gggtttgatc aaggagaaat
    13321 aaaaaccggt cgttacttag cccaatgatt ccactgtagt gtcaaatata catttcttcc
    13381 gcgtcccggc attttttgaa tgttggcgtg ttcacggtaa tcggtatcca aaacattttc
    13441 tacaccccat tgcaattccg cgtgtttatt cacttttatc ccgccacgga ttgtaaataa
    13501 tgtaaaacca tgcgtgcgat cttcaactgc ctgctgtttg gcaaaacggt tttgtgaagc
    13561 ggccgtttta ttagacaatt caaaccaata cgaacccggc acccaacgca cactgtttaa
    13621 aacttcaagc ggcgccatac cgggcatcgg ttcatcccaa gcgcttacgc ggctgtaacg
    13681 gtatgaagct gtaatctgcc atgcgatggt attggtaatg cttcgaaaat gcgacgcttc
    13741 gatacccgcg agatccgcgt gatggatgtt ttcaatttgt ttgaacagcg tatcggtggt
    13801 aatacctgca atatagcgat caatccgtgt gtaaaaaatc tgcgctttgg aaatatgctt
    13861 atccgtcttg tattcgtacg acagttccga ttgataactg acctcgggtt tgagattggg
    13921 attgcctgta taaataaatc cgtcggacac attgtaaaaa taaaaaccat agttctcgat
    13981 atgcgtcggc gtacgcgctg tccgtgcgag cgctaatcgc aatttttgat cggccatcat
    14041 ttgccattca gcggccgcac tggcgctaaa agttatgtat gatgtattga ggtgcgtccg
    14101 gttccaatat ccttccaact cccggcgacc gatatcatca tgcagggaac gaacggaata
    14161 atccaaacga aatgatgatt tgaaaacaat tttttccgtt aacaaacgac ggtattcggc
    14221 ggcgacggcg tagttataga tatgcgttga cccgatattg acgaggtaca tcggcgaaac
    14281 ctcgggatcc aaactttgca tatccatatc ggcaaatgcc gtcatacgat aaaaatccac
    14341 aacccattta atgccttgaa tattgccgcc ggtatagagg ctgctcgaaa aaccgatcgt
    14401 acgcgtattg ccgtacatcg gcatatacat attgggcata aaatcacgtg cggtgacatc
    14461 tctgtcgtag tcatccatat catgatccac catgttaaaa tacactttgg tattcaaaaa
    14521 cgatgcatga tgccaaggct gtctccactg gtgttcgaga ccaaaaatat tggccaccgc
    14581 gcgacgtgta tccatcagca aaacgggata accggcatcg gttgcgacat cgcgaatata
    14641 cgacgccgtc caggtatgat taccgttttg gtgcgcaatg tcggttttaa tgttggattt
    14701 attatatcct gaattatgca tcaaacggcc gtcaccggcg cggaaatcat cggacttgcg
    14761 cagcgaaacg cccgcgcgtg cggcccatgc gccgccggac caattggtgg tcgagcgcgc
    14821 aaaacgcaaa tgagagacgt tttcatatcc ggtttgtacg ccgatggcaa aaggtactga
    14881 aaaatcgggt ttgcccgttc tgagattgag cgatccgccg atcgtctgtc cggcgctcat
    14941 atcaaaaccg cctttagcga cttcgatttt ttcgaggtta tctatttcga catacgacgt
    15001 gacgggatcc attttatcaa tgcacgcgcc gaaaattttc atatcatcca caagcacctg
    15061 aatctggccg ccgct
//
LOCUS       DAOQNQ010000048        14812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1437_length_14812_cov_5.792166, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000048 DAOQNQ010000000
VERSION     DAOQNQ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14812
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1437_length_14812_cov_5.792166"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="PK754_04970"
     CDS             <1..990
                     /locus_tag="PK754_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008285977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNB08886.1"
                     /translation="PFIAVNCGSFNENLIEAELFGYEKGAFTGAHQSKPGRFELANKG
                     TLFLDEIADTSLAFQVKLLRVLQEGVLERVGGIKPIAVSVRLIVATHRDLRKMVREGG
                     FREDLFYRLHGFEITLPSLRERRMDIEVLVASFLKEANARLTFSDELMSWLCQQNWPG
                     NVRQLQSATRRAIVNAGLRGRTFLLPEDFELGTIEKLMPNEDTAERILSAFRKNEFRH
                     RSVSAVASDLSIHRVTVTEYLRGWILRGMSKKNDEEAIMNFLTGGAVVYDVEPLRRRI
                     SEYKSYIESRIDEGLRNNDRPENFVALRYKNLPGEFHEDAEAVYRDYLTNSKL"
     gene            987..2990
                     /locus_tag="PK754_04975"
     CDS             987..2990
                     /locus_tag="PK754_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08887.1"
                     /translation="MRRTNIIYQLAILNALVFAPAKHLAAQSSFLFGNVDSIYTNIRF
                     TGHSTQIAGSGIYYANSDIGISHRRSGWLWDVVARINQAQSYLIDPGHVVIQLPDTVT
                     LRENGDLRSRAHRYTWNNPFSLIAGRQWNKWYIGTEHQLVSRQEVLKEPILREIGNNG
                     RTYYLEWKTSSRTRYDIWSAAHIGYRWNNLTVDIGTTLIPIVGIGKMPKQFDQQTTLR
                     WRLGLAAQHWGGYSRILWSPDVWSASRTQLWTSRIMTGISMTGERILSPRHIMRVDAG
                     YLHRDRWAFYVAVSSSVEKNESLKRASFEDMLRHIHHGSEGSMTPRAMRPRISAGIVY
                     RLDKTKPPVILRVTRSELLQSNIFASKNSYYAYNPVAFIELYNPETVPVDGQIIAEIE
                     DIGIRFQSEALRWQPGEIKRVPIFLYFDPAIFGEGHLAKQLRIGFEKDTYRYQLSAFP
                     VTIHDRHTWDGNTWGLRYFLTPENPIIRSAAKQLYFSEPDTVVTSDLIKFVRLRNLLE
                     KAGKNLRYIEDPVTTIYADQVQYPEETLVKQSGDCEDISVYIASLCMSVGYACGIVDI
                     RPDQNAGVYPSASGRSSPGHVFLLIDTGLSPEWAERAGFSEFDAIARDNTKGETSLWV
                     PIEATLLHEGFIRAHRDGVRQYYDKVIVQQGVSKGSVHIYDLQ"
     gene            2977..3672
                     /locus_tag="PK754_04980"
     CDS             2977..3672
                     /locus_tag="PK754_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08888.1"
                     /translation="MIYNKIYRLIFLTSMVACALYAQDKESWIQKTSGEALYLEPRTG
                     QWIPISAKQTIPLRTYLITHHGSKTTLYDQAEMLVLPDGAYFFVEDAIPKDRDALVGA
                     LTQIEAEHLPNRLESRPVSVGLTYGAPEHKNEEQIPYLAERINAVNRFYELGRKDASL
                     LSLKRLLSKYPRLYRDKKNVEQLFSLYENLELYGYLYDESSRLLNLSQDAEWSVFING
                     WYKTARTYVTQQH"
     gene            complement(3806..6199)
                     /locus_tag="PK754_04985"
     CDS             complement(3806..6199)
                     /locus_tag="PK754_04985"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB08889.1"
                     /translation="MLSNYLTVAWRSITRKKGFSAILISGLAVGMACFLLIALYALDE
                     WSYDRFHGKKDRIYRLAEKLESENSSSNPFPVAQALKTDYPHLVEQTVRLFNFQLTSF
                     TLQIGDQKYNESNLYFADSTFFQVFDYALAEGDPQTALARPNSIIVSRAMAKKYFGDT
                     PPLGKILRLEGKLDLMVSGIFAEIPTQSHIRFDALISFSTAASLTGPNTFKGWVWNPC
                     WTYLLLKEGVSPDELTAQFPEFIKKYYPNFLKAQVTHYLQPLTDIHLHSKLDYEMHPN
                     GDAATVWVFCTVGLLILLISCINFTNLATARSLMRAREVGMRKVLGADRKMIVRQFLG
                     ESLLISLIAWVGALILSEISLPLLNALTGKNFSWAAFQYAWYIPLGLLVAALCGVLGG
                     WYPSLFLARFDAVTVLKGQFKHSGKTRWLRQTLVVMQFAISTGLIAGTIVVYLQLHYM
                     RSADTGFQKEHILVIPTRPAMIKRYETLKSEWKSDSRIQDVTVMNEILGVHHNTHEIR
                     YEPMPVGQWQYFPGLMVDEDFAETFGLQLLAGRDFSKDFPRDDSLGILVNEAAVERMG
                     WGSPEKAIGQIFETIGGHERVIGVVKNFNYKSLAHRIEPFFIDMPNAGNFNFFRKFMA
                     LRIRAHDLPGAIAHIESVWNRIVPEYPCEFSFLDQNIEKMYRAQENLSRIIGYFAGLA
                     IFIACLGMFALASFMAEQRTKEIGIRKTLGARTSELALLFARDFVILVLIAAAVSSLP
                     SAWFMQDWLNNYNFRITLEWWIFASSAGVALLIAALTVSYQAVRTASRNPVKALRYE"
     gene            6415..8487
                     /locus_tag="PK754_04990"
     CDS             6415..8487
                     /locus_tag="PK754_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNB08890.1"
                     /translation="MASKAQTRPDMVRPGNGKKYSLSDFPELTKERLLAAYRHMIIAR
                     YLDDKMLRMLKQGKSFFHIGCSGHEAAQVAAGFAMQPGKDWFYPYYRDQALCLSVGMT
                     AQELMLCFLAKADDPNSGGRQMPQHYGHAGLRIPSQSSPTGTQFLQAVGCAMGAAKEG
                     KDEVVYVSSGDGTTSQGDFHEALNWASREKFPVVFFIQDNKYAISVPIHQQTSDSILS
                     LGAGYSNIHCYEMDGTNFLESYAIIKEATEKARKGEGPALVVADVVRLLPHSSSDDQR
                     KYRPEEDLAKDRARDPIEQMAKFLIDNRILKKEDDEAFRQECYKSVDDAADWAETQPH
                     PDKSVAMKHVFSEEPDTLAYEKTVPSGKPIVLVDAINHAMAEELERDPRIIMFGEDIE
                     DDKGGVFTATKGLSKKFGRDRVFNSPLAESSIVGTAVGLAVRGWKPVVEIQFGDYVWT
                     AMMQIRNELATMRYRSNGTWKCPMVVRIPIGGYIHGALCHSQNIESFFAHIPGLKIAL
                     PSNAADAKGLLKTAIRCDDPVLFLEHKGMYRQGFAQMPEPDADYLLPFGKAAVKREGS
                     DITVVTYGMMVNKCLNAAKYMEETHNVKIEVIDIRTIVPLDTDTILNSVKKTGKCLVV
                     HEDVEFCGFGSEIVAQIMHQGFEYLDAPVQRVAGAFSPVPYNWFLEEMILPQDHNITT
                     ALENLAKY"
     gene            8682..10139
                     /locus_tag="PK754_04995"
     CDS             8682..10139
                     /locus_tag="PK754_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08891.1"
                     /translation="MRKVIFLCLVALLMQDQLSAQTSVGFSDNNPKPIKDYRLPDWGY
                     SRGWINASLSRNNSSSDYSDEEYKNDNSSYWLNSNYILMRESEKQDLNFSASAFYSKR
                     TNETSRNNDLGHSDRDYNDIVAHVMLRGNSSHYAGYYHATILPHIAYTKSESVYKVTD
                     IGYMFPDTAIMRSKEKSNAIDLSLATLFGIGRIRNVTPVIRAMRVHERLNAVGKGESW
                     NEETTQRVAAEFARLYGYQNVYDRHQKEFWNSLDEALPDLTPFEAHYMNDVFQEAIGN
                     RLEGYVIDVGPRIIRYQSRDKRNSSNYYLDDEDVKFRSTYLGMMLNGQFYHNYSINGQ
                     IDFSATGSLDFRSSSKKYSPVSDLKQMQLAVNHLYSIADRLLWTVSGTYDNAHIKIGD
                     EDTHETFKSKKYKISTNLTYFIENRLSISGDAAWLKKKNSPASDLNGYYDMGAMEAIR
                     FVNSGNLFDPYNGVKSSKQWYYGISLTYHFNRTLL"
     gene            complement(10178..10930)
                     /locus_tag="PK754_05000"
     CDS             complement(10178..10930)
                     /locus_tag="PK754_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNB08892.1"
                     /translation="MKVLIIEDEPVAADHVASLLRRIMPNVEIAGQIATVREAVLMLR
                     QNRFDLILSDIQLADGSCFEIFRQTKVNTPIIFITAYDQYALRAFEVFSVDYILKPVT
                     EKKMETALNRFKERRDLFFADYEKLTQPSGKEYKQRFLVRIGSKLIPVECKDILFFYA
                     VDKWIDLITKDNKRFVVDFSLNQLEDLLDPKDFFRINRQYIIHRQALISIAQHQPGQV
                     TALIKSNPSESPVISRRVTPRFKLWLSENKKP"
     gene            complement(10927..11973)
                     /locus_tag="PK754_05005"
     CDS             complement(10927..11973)
                     /locus_tag="PK754_05005"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNB08893.1"
                     /translation="MINLLYPSWLWRMTIAACIGLFSYPVIMLIRGYTIESTTGYYYA
                     GFSLYAACAHAWMIEWHLFKNRRLRNKLSWLDHTWLRFFIELPLAFLIGATGVFIGLS
                     ALMMSFFDAYNLSLTLWSFYLTLGILMSLVYSAFFHVAELLHAWQSAKVRAAEMEQAS
                     VRAQMAALQNQVSPHFLFNSLNMAHALIDRDTEEAKSFIDQLAEVYRYVLKNRHEELV
                     SVQEEIEFARHYLALAQMRFQYILIKESVSPDALQRAIPPVTLQILIENALHHNAITH
                     EHPLQLYIESTDNSLILRNTRNPRLHAPDHSHAGTGLDNIRQRYLFFTERAIQINQTD
                     AMFSVTIPLLEFPA"
     gene            12129..12689
                     /locus_tag="PK754_05010"
     CDS             12129..12689
                     /locus_tag="PK754_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08894.1"
                     /translation="MKILVMTFLVFSAHIAGQTKLKLIFMASDSTTVSEKNEYDSIWS
                     SESKKIVRTMERISGMNFIDTLVMVNVVDMPSNSGGTGTPMRLRSSYPTLIKQGTLIH
                     ELGHRLHFCLQRRTKDFNDHELLFLYLYDVWLDLYGEEFAQLQINAENKRSNKTNNYK
                     EMWEKALKLGADGRKIKLKQMVESQK"
     gene            12708..14015
                     /locus_tag="PK754_05015"
     CDS             12708..14015
                     /locus_tag="PK754_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08895.1"
                     /translation="MKYNIVIKIFMHRIPFFVFCWLITMAAYAQTRMEKYQFHYKEAA
                     TAAKDKNYTKMIYHFERADSLRPNNYGILYNLAAGYALTDQADKVWGPLRSALQVNAG
                     LKFYEDEDFDAVKTDSRFVSTRQLAESLQTVIVKSDMAFTLDDRSFHPEGITFDKKNN
                     LWITGSISQRLIGVVRPDGKLDTTFFKPSKPLYSVMGMRIDSLRRRLWVTTSVMPEMS
                     GFTDSLDGRAAVVCFDADRGHEIARFEVSGGHIFGDLCVTASGSVFISDSKAPEIYHI
                     DTDMKKIVKWKTMDGVWNLQGVDVTHDQQYILIADYVSGIYRLNIKTGEVIKLSFVSS
                     SMLRGIDGLLLDGTAIYAIQNGTNPLRILRLSCDPEWSKIKSVEVIDNNADFLGEPTL
                     GCVSGGSLYYIANSPWGAYDKNHQLDITKAAPPMIRRYTPGVK"
     gene            14018..>14812
                     /locus_tag="PK754_05020"
     CDS             14018..>14812
                     /locus_tag="PK754_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNB08896.1"
                     /translation="MFQPLLLMIYTLAAMVTACEDGPLKPVYPETVTDSTYADRTYIT
                     SVPMLRDGKKYPLVFVFHGAGGNGAGIRSFSGFDKSTWSDSCIFVYPDAKVENWDEGC
                     RCNKPYRLGIDDVGFIDAIYKTMIDKYPVNTRRVYAVGFSQGGLFAHYVACRLSHRFA
                     AVASVASSMNQPLSTYGAPEEKTSVFIIHGKNDGVLPYQGSNEGSFSLLSAPQTLTWW
                     GNYYKMQRTAIGEKAGKNTTLDRYGLESGHEIRLLTPEEEGHQWFFN"
BASE COUNT         4195 a         3075 c         3524 g         4018 t
ORIGIN      
        1 ccgtttattg ccgttaactg cggctcattt aatgaaaacc tcatcgaagc ggagcttttc
       61 ggatatgaaa aaggcgcatt taccggtgcg catcaaagca aacccggacg atttgagctg
      121 gccaacaaag gtacgctgtt tttggatgag atcgccgata catcccttgc ttttcaagtc
      181 aaactcttac gtgtattgca agaaggtgtt ttggagcgtg tcggcgggat aaaacctatt
      241 gcagtgtcgg ttcgattgat cgtcgctacg catcgggatt tgcgaaaaat ggtacgcgaa
      301 ggaggttttc gtgaagattt attttaccgt ttacatggat ttgaaatcac gcttccctcg
      361 ctgcgtgaac gccgtatgga tattgaagta ctggtcgctt cttttttgaa agaagccaat
      421 gcgcgcttga cgttctccga tgaactgatg tcatggttgt gtcagcaaaa ctggccaggc
      481 aatgtgcgtc agcttcagtc tgcgacacga cgtgcgatag taaatgcagg attgcgggga
      541 cgcacattct tactgccgga agattttgag ttaggtacga tcgagaaatt gatgcctaat
      601 gaagatacgg ccgaacgtat cctgtcggcc tttcgtaaaa atgaatttcg tcatcgtagc
      661 gtatccgctg tggcttctga tttatccatt caccgtgtta ctgtaacgga gtatctgcgc
      721 ggttggattt tgagaggtat gagcaaaaaa aacgatgaag aagcaatcat gaatttccta
      781 accggcggcg cggtcgttta tgatgtcgaa cccttacgac gacgcatcag cgaatacaaa
      841 tcctatattg agtcccggat tgacgagggg cttcgaaaca atgatagacc ggaaaatttt
      901 gtcgccttac gatacaaaaa tttacccggt gaatttcatg aagacgccga agctgtatat
      961 agagattatc ttacgaatag taaattatga gacgcacaaa tatcatatat caattggcga
     1021 tcttaaatgc actggtgttt gcaccagcaa aacacttggc ggcccaatct tcatttttgt
     1081 ttgggaatgt agattcaata tataccaata ttcggtttac cggacattcg acacagatag
     1141 cggggtcggg tatatactat gccaattcgg atataggcat ttcacatcgt cgctcgggtt
     1201 ggctctggga tgtggttgcg cgcatcaatc aggcccaatc ttatcttata gacccgggtc
     1261 atgtcgtcat ccaactaccc gatactgtga cgcttaggga aaacggtgat ctgagatcac
     1321 gggcccatcg gtatacgtgg aataatccgt tttctttgat tgcagggcgc caatggaata
     1381 agtggtatat cggcacagaa caccagcttg tatcgcgcca agaggttctt aaagaaccta
     1441 ttttacgcga gatcggaaat aatggtagaa cctattatct ggaatggaaa acatcgtccc
     1501 gcacacgtta cgatatttgg agtgcggctc atatcggata tcggtggaat aatctgacgg
     1561 tggacatcgg cacgacattg attcctattg tgggtattgg aaaaatgcct aaacaatttg
     1621 atcaacaaac aaccttgagg tggcgcttag gattagcggc acaacattgg ggcggatatt
     1681 cgaggatttt gtggagtccc gatgtgtggt ccgcgagccg tacacagttg tggacgagcc
     1741 gtataatgac gggtatctct atgaccggag agcgcatact ttcacctcgt catattatgc
     1801 gtgttgatgc cggatatttg catcgtgatc gctgggcatt ttatgtcgcc gtgtcatcat
     1861 cggtagagaa aaatgaatca ttgaaacgtg cttcgtttga agacatgttg cgtcacatac
     1921 atcatggcag cgaaggaagt atgacgccac gtgcgatgcg tcctcgtatc tccgcaggca
     1981 tcgtatatcg tctagataaa acaaaacctc cggttatctt gcgtgtaaca cgttctgaat
     2041 tgctccaaag taatattttt gccagtaaaa actcatacta tgcatacaat ccggtagcct
     2101 ttatcgaatt atataacccg gaaacagtac ctgtagacgg ccaaatcatc gcagaaatcg
     2161 aggatattgg gatacgtttt caatcggaag cgctacgttg gcagccgggt gaaatcaaac
     2221 gcgtgccaat ttttctctac tttgatccgg caattttcgg agaagggcat ttggccaaac
     2281 aactgcgtat cggatttgaa aaagacacat atcgttacca gctatcggct tttccggtga
     2341 cgatacacga tcggcatacc tgggacggta atacatgggg actgcgttat ttccttacgc
     2401 cggaaaaccc cattattcgt tcagcggcca agcagttgta tttttccgaa ccagatacgg
     2461 tcgttacttc tgatttgatc aagtttgttc gtctcagaaa tttattagaa aaagcgggga
     2521 aaaatctacg gtatattgaa gatccggtta cgaccattta cgccgatcaa gtacagtatc
     2581 ccgaagagac acttgtaaaa caaagcggtg actgcgaaga catcagtgtt tatatagcct
     2641 ctctgtgcat gtcggtggga tatgcatgtg gtatcgtaga tattcgcccc gatcaaaatg
     2701 ccggtgtcta tccttcggca tccggccgat catcgccggg gcatgtgttc cttttgatag
     2761 ataccggttt atcgcccgaa tgggcggagc gtgccggatt ttccgagttt gatgcgatag
     2821 cgcgcgataa tacaaaaggt gaaacatcgc tttgggttcc gatcgaagcg acgttgctcc
     2881 atgagggttt cattcgtgca catcgcgacg gtgtgcgcca gtactacgat aaagtcattg
     2941 tacaacaggg cgtaagcaaa gggagtgttc atatatatga tttacaataa aatctaccgg
     3001 ttaatctttt tgacgagtat ggtcgcgtgt gcattatatg cgcaggataa agaaagctgg
     3061 atacaaaaaa catctggtga agcactttat ttggaaccgc ggaccggtca atggataccg
     3121 atttcagcaa aacaaaccat accgctgcgc acgtatctga tcacacatca tggatcgaaa
     3181 acgacattat acgatcaagc ggaaatgctg gtgctgcccg atggggcgta tttttttgta
     3241 gaggacgcca tacctaaaga tcgcgatgct ttagtgggag cgcttactca gatcgaggcg
     3301 gaacatttac caaatcgttt ggaatcgcgc cccgtttcgg ttggtcttac ctacggagcg
     3361 cctgaacata aaaacgaaga acagattcca tatctcgctg aacgaattaa tgctgtaaat
     3421 cggttttatg aattgggtcg taaagatgcg tctttgttaa gtctcaaacg actcttgagc
     3481 aagtatcctc ggttgtatcg agataaaaag aacgttgagc aactctttag tctttatgaa
     3541 aatttggagc tttacggata tttgtatgat gagagttcgc gtttgctcaa tctgagtcag
     3601 gatgccgaat ggtcggtttt tatcaatggt tggtacaaaa cggcaaggac gtatgtgact
     3661 caacagcatt aattcatcta tcttaatttt ttcgtagtag tctaaaaact caatcactgg
     3721 tatccttaga ggcttatata tattgtattg atggacaacc aacagtcact catatcggtg
     3781 aatgtgaatt tagtgagttt ctttactact cataccgtaa tgctttcacg ggattgcggg
     3841 aagccgtacg tacagcctga taactgacgg taagtgcggc gatcagtagc gcaacaccgg
     3901 ccgaggaggc aaaaatccac cattccaacg tgatacggaa attgtaatta ttcagccaat
     3961 cctgcataaa ccatgcggaa ggaaggcttg ataccgctgc ggcgatgagt accaagatga
     4021 caaaatcccg tgcgaaaagt agcgcgagtt cggaggtgcg tgcgccgagc gttttacgaa
     4081 tgcctatttc tttggtgcgc tgttcggcca taaaagaagc cagcgcaaac ataccgagac
     4141 aggcaataaa aatagccagc ccggcgaagt atccgataat ccggctcagg ttttcctgag
     4201 cgcgatacat cttttcgata ttctgatcaa gaaatgaaaa ttcgcacgga tattccggaa
     4261 cgatgcgatt ccacaccgac tcaatgtgcg cgatcgcacc ggggagatca tgggcgcgaa
     4321 tgcgaagcgc catgaacttt ctgaagaaat tgaaattacc ggcattgggc atgtctataa
     4381 aaaaaggctc gatacggtgt gcgagtgatt tgtagttaaa atttttgact acaccgatca
     4441 cgcgttcgtg accgccgatg gtttcaaaaa tctgaccgat cgctttttcc ggagaacccc
     4501 agcccatgcg ttcgaccgcc gcttcattga ctaaaatacc cagcgaatca tcgcgcggaa
     4561 aatcttttga aaaatcccgt ccggccagca gttgcaagcc aaatgtttcc gcgaaatctt
     4621 cgtccaccat gagacctgga aaatattgcc attgaccgac aggcataggt tcataacgaa
     4681 tctcatgggt attatgatgc acacccagga tttcattcat tacggtgacg tcttgaattc
     4741 ggctatcgga tttccattcg gacttcagcg tttcataccg tttgatcatc gccggtcgcg
     4801 taggaataac gaggatgtgt tctttttgga atccggtatc ggcggaacgc atataatgta
     4861 gctggagata gacgacgata gtgccggcaa taagaccggt agaaatggca aattgcatca
     4921 ccacgagagt ctgtctcaac caccgcgttt taccggaatg tttgaattgt cctttgagaa
     4981 ccgtcacggc atcaaatcgt gctaaaaaca aggacggata ccatccgccc aaaactccgc
     5041 acagcgcggc gactaatagt ccgaggggta tgtaccatgc atattgaaac gcggcccaac
     5101 tgaaattttt ccccgtgagc gcattcagca gcggtaatga tatttccgac agaataagag
     5161 cgccaaccca tgcgatcaaa ctaatcaaaa gcgattcgcc gagaaactga cgtacgatca
     5221 tctttcgatc cgcgccaagc actttgcgca taccgacttc ccgtgcgcgc atcagcgaac
     5281 gtgccgtggc gagattggta aaattgatac acgagataag caaaatgagc aaaccgacgg
     5341 tgcagaatac ccatacggtg gccgcatccc cgttggggtg catttcataa tctaacttgg
     5401 aatgcaagtg aatatcggtc agaggctgga gatagtgcgt aacctgagct ttgaggaaat
     5461 tgggataata tttttttata aattccggaa attgagcggt caattcatcg ggtgaaacgc
     5521 cctctttcaa aagcaagtat gtccagcacg gattccaaac ccagcctttg aatgtattcg
     5581 gtccggtcaa ggaagccgcc gtagaaaacg aaatcagcgc gtcaaatcga atatggcttt
     5641 gggtcggtat ttccgcaaaa ataccggaca ccatcagatc aagtttgcct tctaatcgaa
     5701 gtatttttcc caacggcggc gtatcaccga aatatttttt ggccatggcg cgggatacga
     5761 tgatactatt ggggcgggca agcgctgttt gcggatcacc ttccgctaag gcgtaatcaa
     5821 atacttgaaa aaaagtcgaa tcggcaaagt acaaattaga ttcgttgtat ttttgatcgc
     5881 cgatctgcag cgtaaaagaa gttaattgga aattaaacag acgtacggtt tgctccacaa
     5941 gatgcggata gtcagttttc aaagcttggg ctaccggaaa cgggttactt gatgaatttt
     6001 ccgattcgag tttttctgct aaacgataaa tgcgatcttt tttgccgtga aagcgatcgt
     6061 aggaccactc atccaatgcg tacaaagcaa taagcagaaa acaagccatg ccgacggcta
     6121 agccggaaat aagaatagcg gaaaaccctt ttttgcgggt aatagaacgc catgcgaccg
     6181 tcagataatt ggagagcata tacgatgacc tcaaactaaa atgatatata aaatagaatg
     6241 gtgttaaacg aaaggttgat ggtaaaaacg ataaaataaa gaatttgttt cggttggagg
     6301 cgaatatttt atctgctaat ttccgcttgt gcatataggt ttttttacct acaatcccgc
     6361 gtttcagact atttttgtat aactcaaatt attgtacgaa taaggagtaa acgcatggct
     6421 tccaaagccc agacccgtcc cgatatggtc aggcccggta acggaaaaaa atattcgctg
     6481 agcgattttc ccgaattgac gaaagaacgg ctgttggccg cgtatcgtca catgatcatc
     6541 gcacgatatt tggatgacaa aatgttgcgt atgctcaaac aaggaaagag tttttttcat
     6601 atcggatgtt ccggccacga agccgcgcag gtggcggcgg ggtttgccat gcaacccggc
     6661 aaggactggt tttatccgta ttatcgcgac caggcgctct gcctcagcgt aggtatgacg
     6721 gcccaggaac tcatgttatg ttttttagcg aaagccgatg atcccaattc cggaggtcgt
     6781 cagatgccgc aacactacgg gcatgcgggt ttacgaattc cttcgcaatc cagtcccacg
     6841 ggaacacagt ttttgcaggc agtcggttgt gcgatgggcg cggctaaaga gggcaaagac
     6901 gaagtcgtgt atgtgtcttc aggggacggt acgacgtctc aaggtgattt tcacgaagcc
     6961 ctcaattggg cgagccgtga gaaatttcct gtggtgttct ttattcagga taataaatat
     7021 gcgatttccg taccgatcca tcaacaaaca tcggattcga tcctctcgct cggcgccgga
     7081 tattcgaata ttcattgcta tgaaatggac ggaacgaatt ttctagaaag ctatgccatc
     7141 ataaaagaag cgacagagaa agcacgcaaa ggcgaaggac cggctttggt tgtggcggat
     7201 gtcgtccgtt tattaccgca ctcctcttcc gatgatcagc gtaaataccg tccggaggaa
     7261 gatcttgcga aagatcgtgc gcgcgatccg atcgaacaaa tggccaaatt tctcatcgat
     7321 aaccgcattc ttaaaaaaga agacgatgaa gcgtttcgtc aggaatgtta taaatctgta
     7381 gatgacgcag cggattgggc cgaaacgcag ccgcacccgg ataaaagcgt tgccatgaaa
     7441 catgtgtttt ctgaagaacc ggatacgctc gcatatgaaa aaaccgtacc gagcggaaaa
     7501 cccatcgtgc ttgtggatgc gatcaatcat gctatggccg aagaactgga gcgcgatccc
     7561 agaattatta tgttcggtga agatatcgaa gatgacaaag gcggcgtatt tacggcaacg
     7621 aaaggcctgt ccaaaaaatt tggacgcgat cgcgtgttca attcaccgct agcggaatca
     7681 tccatcgtcg ggacggccgt agggctcgcc gtgcgcggat ggaaacctgt ggtagagatt
     7741 cagtttggcg attatgtgtg gacggcgatg atgcagattc gcaacgaact cgcgacgatg
     7801 cgttaccgtt ccaacggtac gtggaaatgt ccgatggtcg tgcgtattcc tataggcggt
     7861 tatattcacg gtgcgctttg ccattcgcag aatatcgaat ccttttttgc acatattccc
     7921 ggtttgaaaa tcgcattacc gtcgaatgca gccgatgcga aagggcttct gaaaacggcc
     7981 attcgttgtg atgatccggt gttgttcttg gaacacaaag gcatgtatcg tcaaggtttt
     8041 gcacagatgc cggaaccgga tgcagattat ttgcttcctt tcggcaaagc ggccgtcaag
     8101 cgtgagggtt cggatatcac ggtcgtgacg tacggtatga tggtcaataa atgcctcaat
     8161 gctgcgaagt acatggaaga aactcacaat gtgaaaatcg aagtgattga cattcgtacg
     8221 atcgtgccgt tggataccga tacgattttg aattccgtca agaaaacagg caaatgcctt
     8281 gtggtgcacg aagatgtcga attttgtggt tttggcagcg aaatcgttgc gcagatcatg
     8341 caccaaggat ttgaatacct cgatgctccg gtccaacgtg tggctggcgc tttttcaccg
     8401 gtgccgtata actggttcct cgaagaaatg atacttccgc aggatcataa cattacgacc
     8461 gcgcttgaaa atttagcaaa gtactaggat tgatttgtaa ctcccgccgt atgttctcca
     8521 tgcggcggga tgatttttta ttaaacctta cggccttatc tctgtctaat acagatacat
     8581 tcctctttta cttaacatag gtagtcaatg atttgtttgc tataacggtt tcgtagtttt
     8641 taggtcaatc tttcattttt cacaaattag agggaattct catgagaaaa gtcatttttc
     8701 tttgcttagt tgcactatta atgcaggatc aattatccgc acagacaagt gtaggctttt
     8761 cagataataa tcccaaaccg atcaaggatt accggttgcc cgattgggga tatagccgcg
     8821 gatggatcaa tgcgagcctg agtagaaaca atagctcttc ggattattct gatgaagaat
     8881 ataagaatga taactcgtcg tattggctaa attccaatta tatccttatg cgtgaatcgg
     8941 aaaagcagga tttaaacttt tcagcttcgg cattttattc caaacgaacc aatgaaacca
     9001 gtagaaataa cgatctaggt cactctgacc gtgactataa tgacatagtt gctcatgtta
     9061 tgttgcgagg aaatagttcc cactatgcgg gatattatca tgccaccatt ctaccgcaca
     9121 tagcttatac aaagagcgag tctgtttata aagtaacgga tatcggttat atgtttcctg
     9181 atacggccat aatgcgttct aaagaaaaat caaacgccat tgatttgtct ttggcgacat
     9241 tgtttggtat tggccgaatc cgtaacgtga cacctgtcat tcgcgccatg agagtgcatg
     9301 aaaggctcaa cgctgtcgga aagggtgaat catggaacga agaaacaacg cagcgtgttg
     9361 ccgcagagtt tgcacggtta tatggttatc aaaatgtcta tgataggcat caaaaagaat
     9421 tctggaactc gttagatgaa gcattaccgg atttaacgcc gtttgaagcg cactatatga
     9481 acgatgtttt tcaggaagcg atcggaaatc gtttggaagg ctatgtgatt gatgtcggac
     9541 cacgaattat tcgctaccaa agccgagata agagaaattc gagcaattat tatctggatg
     9601 atgaagacgt gaagtttcgt tccacttatt taggaatgat gctcaacggg caattttatc
     9661 ataattactc cattaatggg caaattgatt tttcggcaac aggtagtttg gattttcgat
     9721 caagttcaaa aaaatatagc cctgtcagtg atcttaagca aatgcagttg gccgtcaatc
     9781 atctgtattc cattgcggat cggttattat ggactgtcag cggcacatac gataatgcac
     9841 atattaaaat cggcgatgag gatacacacg aaacatttaa aagtaagaaa tataaaatta
     9901 gcactaactt aacctatttt atcgaaaaca gattaagtat ctcgggggat gccgcatggt
     9961 taaagaagaa aaatagcccg gcatccgatt tgaatggtta ttatgacatg ggtgcaatgg
    10021 aggctatacg gtttgttaat agcggaaatc tgtttgaccc ttataatggc gtgaagtcgt
    10081 caaaacaatg gtactatggc attagcttga cctaccattt taaccgaact ttgctataat
    10141 acatcatcat atttaactat aaatcctgat gtcaccgtca gggtttttta ttttcggata
    10201 accatagttt gaagcggggc gtaactcggc gactgatgac cggggattcc gatggatttg
    10261 atttgataag cgccgttact tgtcccggtt gatgctgagc gatggagatc aatgcctgcc
    10321 tgtgaatgat gtattgccga ttgatgcgaa aaaaatcctt cgggtcaagt aaatcttcca
    10381 attgattgag gctgaagtct acgacaaaac gtttattgtc ttttgttatg aggtcaatcc
    10441 atttgtctac agcataaaaa aacagtatgt ctttgcattc cacaggaatc aatttacttc
    10501 caattcgcac caaaaaccgt tgtttatatt ctttacccga gggctgcgta agtttttcat
    10561 aatctgcaaa aaacaaatca cgtcgttctt tgaatcgatt taaggctgtt tccatttttt
    10621 tttcggttac cggtttcagt atgtaatcca cactgaatac ttcgaatgcc cgtaatgcat
    10681 attgatcgta ggctgtaata aaaataatcg gcgtattaac cttggtttgt ctgaaaatct
    10741 caaaacacga cccgtcggct aattggatat ccgataagat cagatcaaaa cgattttgac
    10801 gaagcataag tactgcttcg cgcaccgttg cgatttgtcc tgcgatttcg acgttcggca
    10861 taatacgccg gagcaacgag gccacgtgat ccgcggcgac cggttcgtct tcgatgatca
    10921 ataccttcat gcaggaaatt ccaataatgg tatggttact gaaaacatcg cgtccgtttg
    10981 attaatctgt atcgcacgtt ccgtaaaaaa aagataacgt tgccgaatat tgtctagccc
    11041 tgttccggcg tgggaatggt caggagcgtg caatcgagga tttcgcgtat ttcgtaaaat
    11101 gagcgagttg tccgtggatt cgatataaag ttgcagagga tgttcgtgcg taatggcatt
    11161 gtgatgcaag gcattttcaa tcagaatttg tagcgtaacc ggaggaatgg ccctttgcag
    11221 ggcatccggc gaaacagatt ctttgattag gatgtattga aatcgcattt gagcaagcgc
    11281 aagataatgg cgagcaaatt cgatttcttc ttgtactgaa acgagttcct catgccggtt
    11341 tttcagaacg tagcgataca cctcggcgag ttggtcaatg aatgatttag cctcttctgt
    11401 gtcgcgatcg atcaaagcat gcgccatatt taaactatta aacagaaaat ggggactgac
    11461 ttgattttgt aaggctgcca tttgtgcccg aacggaggct tgctccattt cggcggcacg
    11521 tacttttgcg gactgccagg catgcaataa ttcggcgaca tgaaaaaatg cggaatatac
    11581 cagcgacatc agaataccca acgtcaagta aaatgaccag agtgtcagac tgaggttgta
    11641 ggcgtcaaaa aaactcatca taagcgccga cagcccgata aaaacccctg ttgctccgat
    11701 cagaaaggcc aacggaagct caataaaaaa acgtaaccag gtatgatcaa gccatgatag
    11761 cttgttccgc aaacggcgat tcttaaataa atgccattcg atcatccagg catgggcaca
    11821 agcagcatac aatgaaaaac cggcataata atatcctgtg gttgattcga tcgtataacc
    11881 gcggataagc atgatcacgg gatatgaaaa taaaccgata catgcggcaa tcgtcatgcg
    11941 ccacaaccat gagggataca ataaattaat catacgacca atataatcgg attttccttc
    12001 ataaacatac cattccctcc aaaaaaatga cgtttcacgt caccgatatc ttttacagtg
    12061 ataaagtcac cgtatattgt gtgatcaggt tgatcaattc atgtttcata tatccgataa
    12121 ggctggttat gaaaatttta gtcatgacgt ttcttgtttt ttcggcgcac atcgctggac
    12181 agacaaaact aaaattgatt tttatggctt ccgattcaac tacagtttca gaaaaaaatg
    12241 aatacgactc tatatggtct tccgaaagca aaaaaatagt tcgaacgatg gagcgtattt
    12301 ccggcatgaa ttttattgat acacttgtaa tggtgaatgt cgtcgatatg cccagtaatt
    12361 caggtgggac aggcacgcca atgcgattga gatcaagtta ccctactctt atcaagcaag
    12421 gtactttgat tcatgagtta ggacataggc ttcatttttg tttgcaacgt cgtacaaagg
    12481 atttcaacga tcatgaatta ttattcttat atctatatga tgtttggctt gatttatatg
    12541 gggaggagtt cgcccaattg cagattaatg cagagaacaa gagaagtaat aaaacaaata
    12601 actacaaaga aatgtgggaa aaagctttaa agttgggcgc tgacggcaga aaaataaaat
    12661 tgaaacaaat ggttgaatct caaaaataaa acaaagaagc aatttgtatg aagtataata
    12721 ttgtaatcaa aatttttatg catcgaattc cattttttgt tttttgttgg ttaataacaa
    12781 tggcggcgta tgctcaaact cgtatggaga agtatcagtt tcactacaaa gaagcagcca
    12841 ctgccgcaaa agacaaaaat tacacaaaaa tgatttatca tttcgagcgt gctgattctc
    12901 tgcgaccaaa taattacggt atattatata atctggccgc cgggtatgcg ttgacagatc
    12961 aggcagataa agtatggggg ccgcttagat cggctttgca agtgaatgcg ggattaaaat
    13021 tttatgaaga cgaggatttt gatgccgtca aaacggattc gcgatttgta tccacccggc
    13081 aattagccga atcactccaa actgtaattg taaaatctga tatggctttt acactggatg
    13141 accgtagttt tcatcccgaa gggattactt ttgacaaaaa aaacaatctc tggattaccg
    13201 gaagtattag tcagaggtta atcggggtag tgcgcccgga tggaaaattg gacaccactt
    13261 tttttaaacc ttcgaaacct ctgtattcag tgatgggcat gcgcatagat tcactgcgtc
    13321 gtcgcttatg ggtgacaaca tcggtcatgc cggaaatgtc agggttcacg gattcacttg
    13381 acggacgtgc tgcggttgtt tgttttgatg ccgatcgcgg tcatgaaatc gctcggtttg
    13441 aagtgtcggg tgggcatatt ttcggtgacc tttgtgttac ggcttcaggt tcggttttta
    13501 tcagcgacag taaagcgccg gagatttatc acattgatac ggatatgaaa aaaatagtaa
    13561 aatggaaaac gatggatggt gtatggaatt tgcaaggcgt cgatgtaacg cacgatcagc
    13621 agtacatcct catcgccgat tatgtttccg gtatctatcg ccttaatatc aaaacaggtg
    13681 aagtgataaa actatcattt gtatcgtcgt cgatgttgcg tggtattgac ggattacttt
    13741 tagacggtac ggccatctat gcgatccaaa acggtacaaa tccgttacgc atcctgcggt
    13801 tgagctgcga tccggagtgg agtaaaatca agtccgtcga agttatagat aataatgccg
    13861 attttttggg cgaacctacg ctagggtgcg tatccggcgg atcgctgtat tatatcgcca
    13921 atagcccgtg gggcgcttac gataaaaatc atcaattgga tataaccaag gccgcacctc
    13981 cgatgattcg tcgctataca ccgggagtca aatagccatg ttccagcctt tacttcttat
    14041 gatatataca ttggctgcaa tggtgacggc atgcgaagac gggccgctca agccggtgta
    14101 tcctgaaaca gtgaccgatt cgacatatgc cgatcgtaca tatatcacat cggtgccgat
    14161 gctacgcgat ggaaaaaaat atccgctcgt ttttgttttt cacggcgccg gcggaaacgg
    14221 cgcgggtatt cgttcgttct ccggttttga caaaagtacg tggagtgatt cctgtatctt
    14281 tgtgtatccg gatgccaaag tcgaaaattg ggacgaaggc tgccggtgta ataaacccta
    14341 ccgtctcggc attgacgacg taggttttat tgatgcgata tataaaacga tgatagataa
    14401 gtatcctgta aatacgcgtc gtgtatatgc tgtcggattt tcgcagggcg ggctttttgc
    14461 ccattatgtg gcttgccgtt tatcccatcg ttttgccgct gttgccagtg tggcatcgtc
    14521 tatgaatcag ccgctctcca cctatggtgc gccggaagaa aaaacttcgg tgtttatcat
    14581 tcacggtaaa aatgatggtg ttttaccgta tcaaggttcc aatgaaggca gtttttcgct
    14641 gctttctgcg ccacagacat taacgtggtg gggcaattat tataaaatgc agcgtacggc
    14701 aatcggcgaa aaagcaggaa aaaatactac gttggatcgg tatggtttgg aatcgggtca
    14761 tgaaatccga ctgctgacac cggaagaaga agggcaccaa tggtttttta at
//
LOCUS       DAOQNQ010000049        14582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1468_length_14582_cov_5.139671, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000049 DAOQNQ010000000
VERSION     DAOQNQ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14582
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1468_length_14582_cov_5.139671"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="PK754_05025"
     CDS             <1..926
                     /locus_tag="PK754_05025"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNB08897.1"
                     /translation="ALQNQDIIAIVPMRSQDRTKGVLLLGPKISGKGFTKEELDFVFT
                     LGNQAMISLENAWLFEDSLERKRLEEELELARKIQMGLLPKDFPKLNNFEVYGLNISS
                     KQVGGDYFDVLKINETQYIIAIADVSGKGVPASLLMSNVQASLLALSTEVSDFSKLVY
                     KVNNIIYANTDSEKFITFFGGVLDIEKRTFTYVNAGHNPPYWRKTDGTLHTLDKGGLL
                     LGMLPDMPYEQETITLSPGEGIVMFTDGVTEAMNEKLDQYDESRLEALIKTHPKITAK
                     ECTKKIAAEVKTFAGAAPQSDDITVVILHAI"
     gene            997..3342
                     /locus_tag="PK754_05030"
     CDS             997..3342
                     /locus_tag="PK754_05030"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB08898.1"
                     /translation="MNKMLVKNLFFLALFVTVAVVNSQETGVITGVVIDAKTKEYLAG
                     VNVQILGTTRGTVADINGQYRLDKLADGTYRLQFSLIGYAPLIKTDVIVTGNKPFRLN
                     VAMEAESIEGDEVIVTAGYFEEQNQTKTSTANLSREEIRRFPGGFEDVVQTVATLPGV
                     AVNGGGGRNDLLVRGGGPSENLYIVNGLEIPNINHFGTQGNNGGSLSFINLDFVDNVD
                     FSTGGFSARYGDKMSSVTQLTLRSGRDDRIGGKGLVSATQFGVNTEGPLGKRGNFIMS
                     ARRSYLDFIFKAAGFAFVPRYTDFNMIGQYEPSPRDKFTFLALTALDDVSKNNEKRKD
                     RIFNSGLLDNEQFIQVVGAGYQHNLEKGFWQVTANVNFSGYRFSQKDTTLREYYNSRA
                     NEVETNASVQRYFVINKSMGILTGLSMKFVNNRNETVFGDTIVDRNGNRIPADSLGLA
                     GLDETRASAYKYGGFTEMDWVVSPRLNANAGLRVDRYSALKKTTYVAPRLAFKYKISD
                     KSNLKLSGGTYYQSPSYVWLVNPANKKLKALRNDMVVAGADRLLRPDVKLSIETYYKK
                     YSHLPTGTQKGINDYIVITSTGTGFGGREDNFQSFGYATLVSRATGKAFGVEMLLQKK
                     FSDIPCYGQVSITVGKSTVVAGNGKTYPNPFDQRFILNISGGYKLNTKWEFSGKFRYF
                     TGAPYTPVYRPQDNPVQPGVVQNLPDEYLSKRLGASHHLDLRADRYFNYEHWRLIIFM
                     DVQNVYNYKVKTPPSYDFAEDKVTRGDRIGILPTIGISAEF"
     gene            3359..4957
                     /locus_tag="PK754_05035"
     CDS             3359..4957
                     /locus_tag="PK754_05035"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNB08899.1"
                     /translation="MPKILICVIALNLTGCGFFITPRSSDQVSIREENLVAHLTYLAS
                     DELEGREAGTRGEKLAAGYIASQLRQNGVKPFALARTADGRDSLSYFQEFSSTKIAIG
                     KHSSVIFYDSASGNSFTMPYAETFSNFHEYVDNCNVSAPLVFAGFGITAPEYGYDDYA
                     GLDVRGKVVLVLDGEPTSEDDQFFDGKIVSSYSSALFYKRHRAKELGAAALFVLSYQA
                     LLEKWDEYVDFFGSSRMIFDPSLLPKSEGERVPFYYCHTVFAKRALASAETGFEQIQS
                     KAQSGAMVPRFTVKNIRCDVRIERTVTQVKAMNIIGKIEGHDPVMKNEYVAIGAHFDH
                     LGVNASGAIFNGADDDGSGTAAVLETVRSVFASQQNKRSVLVVFHGAEEKGLIGSKYL
                     TDPATVNAWTWKDVTAQINMDMVGRESADSIYVIGSGRLSSQLQSTNETINRGTGYFY
                     FDYTFDRDDDPNQYYYRSDHYNYAKLGIPIVFFFDGMTSDYHKPSDDVAKINFKKLYK
                     ITDHTRRLLMNLSNRPERLVVDKH"
     gene            4961..5554
                     /locus_tag="PK754_05040"
     CDS             4961..5554
                     /locus_tag="PK754_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08900.1"
                     /translation="MFLRRKEINIYEFLRPGSRGHGYVVNFHRKYFDVFRGTDLENQD
                     AFINEVFIGLSSIREESIREKADHYLMKAVYFRCWRLFEDNKAYKQSEVPASRAQRDD
                     EDDDAVTRAPSTTPLPDSPLLAQDLLEHIHRFRMTLNPREIVVLNGLIDEVSIAEIAR
                     KAGVTANYAGVLIHRVRESLHVYLEDIGYFKKNKKKL"
     gene            5620..6669
                     /locus_tag="PK754_05045"
     CDS             5620..6669
                     /locus_tag="PK754_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08901.1"
                     /translation="MTDDEKKINLDFLDPLRRSPSVIWNAEMMSRCIHGYSVMSKHER
                     DWFDTQIREEDSARNLFEQVFDDDILLDPSYRTESPLIQKMSKGIQIRCATGLTLQID
                     QSMALISEAGPAWIGQSALIKSVGWSLRILEVQTGLRLPCALLSEQFSSELKVCSVPL
                     ADKEDSVHTDRPVWYIWSAAATIVVMIMAGMWWLQKPTEVLTTDTKLDISKDTLVVPQ
                     RNFTEQPQVELPLRQVPKPNKEKLLAAHFEPNAVLENFIGRTYRAEVRIEIIQPSSGD
                     TLRGPLVFTWKKSDPNVNVTVNILNNRNASIWEANTKEDQLSYNGVLAPGLYYWIIKS
                     GDVRLDVRKFYIPIQ"
     gene            complement(6663..9737)
                     /locus_tag="PK754_05050"
     CDS             complement(6663..9737)
                     /locus_tag="PK754_05050"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNB08902.1"
                     /translation="MSKLSFQIFIFGFIITFTLQSQTENDSFKAAQSHATAQTYLKGL
                     KYDSAIVFLKDALKLYIKTENWKKAGTVYDEITYGQRISGFFMDATATATEGLALVKS
                     KLGDSNIVYGELLNTYATVLWSRGLYDSSLNINRRNLKLRTALLGPDHQDVGAVHTNI
                     GLAYKDKGYYEEALSHYEKGLKIRIKALGENHSSIASSYINMGVIYRIRGDYKTAEEL
                     YLKALNIRIKTIGEKHPRTAIIYFNIGICYDAMGDYERAMMFYRKAIDVALSTLGPEH
                     PDVSDMYIGIAICYYNQGMFHESLEYEKKALVIRLKRHDPSSVILAINYNNIGNVFRE
                     LKQIDSAEYYYQLAFTINKREYGSHSEVAKGYVYFGDIAERREHWREADSLFTLGLNM
                     AKDVLGFKNPLVAEILSRHASLAKKTNDITAAIRLSQAALNASLIGFNDSNILSNPTL
                     KDATLESVSIEILAQKARLWHQLFLTKHDVRHMEESFKLFELVSGWIDYLRQGYRLEG
                     SKIFLGTKAKRIYEEGIRACVTAYRATQAPLWIERAFTLSQRSKSGALREALHDLQIR
                     NFTQMPDSVSNLERDIKINLASYETQLQNQLQLHDSSKHSEIQRLERIIFYQKAQYDS
                     LQNWIEQNNPEYFKLKYKSTIPTIAEIQHTLKKDAAVLIDYFTGDSITTVITVTATRI
                     SMQVLHNDTTLESSVRIIRKHVNNLASDELSQSSYSFYKRWVQPSLQSVKLGATLVII
                     PDGSLSYLPFETLVTSSVSSNLTSKVRYLCQDFPIQYVFSADRIITTSDKPPTNAFFG
                     FAPVFSDTTISGYIKEYQFDDSTMSSHRGFFTRGTHFAQLPHSQTEVTNIQELFSNRK
                     FNSKIVTHQKAVKSVLTSDSLTNYGFIHLASHGFINEANPKLSGIALYSDDSVDVKED
                     VLYAGEIYNLRLNAHLVTLSACKTALGKIEKGEGVIGLTRGFIYAGARHVLVSLWPVD
                     DRLTAEFMTEFYRNVFTEKNLSLALQKTQKRWIAQQHTSYPGDWGAFVLIGY"
     gene            complement(9734..10702)
                     /locus_tag="PK754_05055"
     CDS             complement(9734..10702)
                     /locus_tag="PK754_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB08903.1"
                     /translation="MPLIETSTYRAPLLLFNGHVQTIFPTLFRKVHDVSFTRERITTP
                     DDDFLDLDWLTMNASSKLVILSHGLEGNTSRAYVRGMAKRFHEEGWNILAWNYRGCSG
                     ESNRLLRSYHSGATEDLEVVIHHGLSRGYTEIVLIGFSLGGNMTLKYLGEKGNQVHPA
                     IRKAVTFSVPVDLKSSALTMESPACRIYMRRFLRMLHEKIKLKSTMFPGQISDTGFSE
                     IKTFRQFDDRYTAPLHGFRDAEDYWSRCSAVRFLDKITIPTLLVNAQNDPFLGPSCFP
                     ITQVQHHTHITLEMPRTGGHVGFPASSKKGYYWSEERALLFTNEGV"
     gene            complement(10710..12905)
                     /locus_tag="PK754_05060"
     CDS             complement(10710..12905)
                     /locus_tag="PK754_05060"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNB08904.1"
                     /translation="MKKRLLWIIAVMITVPVYAQTNVTLTPEYLYNGEIKQRTEAPPY
                     FWTSQNTIILYDTRVAWPDRSYQMLDPQSGERFKLFDMRTAIAGLQKHISSVPAALPW
                     PEGLDASGSLALFSFEGDVFIFHFKTSQWTRVTATSEEEMAPRLAPDGKKVVYVRHND
                     LFSYDLVSQKETQITSDGSSTLLNGRLSWVYWEEIYDREELGSFWSDDGSLLAFYKFD
                     ESQVDEVVFQDYKPAVPALIRQRYPKTGSVNPIATLHCTNMNTGKTVAVDMTGLTYEY
                     IVRVKWLPDNKRLAMQTMNRSQTELNLYFVDAASGRPTRILTEINSGFVREHNDLYFL
                     KGGSEFIWSSQRSGYTHLYHYQSNGRLIRPVTSGTWSVQMTTLYSKGGVVGIDEKTQT
                     LYYQSLEKSSIERHIYSIRFDGKNKKRLSIKDGTYDATLSPDGRFLMYHHSSADTPPS
                     LTLHKPDGSIKQVIAAADTTYARTLGFVPYRFFTIATRDSFPMPAMMLRPADFDSTKK
                     YPVILHVYGGPSAPQVNHRWPTETIMPHQLWVRDGYIIITVDNRSATGISKILENTVV
                     RMQASDGELRDLEDAVAWIKTRSFVDPERIGIWGRSGGGTFTLLAMTRTSLFKAGVAV
                     APLTSWHYYDTKYGEATMKTPQENPDGYLKTDLNRYAKNLSGRLLLVHGTYDDNVHPQ
                     NTWHFVDELIRADKRFDMMMYPMRKHTFTDTAAKLHLCKTMIEFWKRNL"
     gene            complement(12933..13652)
                     /locus_tag="PK754_05065"
     CDS             complement(12933..13652)
                     /locus_tag="PK754_05065"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNB08905.1"
                     /translation="MLSNQALDPHFRWRGHEVTRVEAFSDAVFAFAMTLLVVSLEVPK
                     NLSQLLETMRGFPAFATCFTILVMLWYWHYKFFRRFGLQDRFTIVWNAALLFVVLFFV
                     YPLKFVMTLVVNMMLGVAYEQPPIAAGQGDDMMYIYNAGYIAVCGIYALLYHHAYRLR
                     ARFELNEAEVFIARSERRAYIINIGIALFSTSLLLYGGSAMANWAGLTYWLIGPAMGI
                     HGYRTGKKLHAIQLRMNPNNA"
     gene            complement(13698..14249)
                     /locus_tag="PK754_05070"
     CDS             complement(13698..14249)
                     /locus_tag="PK754_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018619863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNB08906.1"
                     /translation="MHRNWLISRLNAYHPSDPHESAMRDRMLDFVQTYPDCFERYLSI
                     GHITGSAWIVNPSRDKVLLTHHGKLNRWLQLGGHADGDPHVDRVALREAQEESGVEAT
                     TLLSDMPFDLDIHEIPPHKSDAAHLHYDVRFLIEIDDAIPLRISSESKDLAWIALDRI
                     ADYTTEESVLRMARKTAVMQNNL"
     gene            complement(14253..>14582)
                     /locus_tag="PK754_05075"
     CDS             complement(14253..>14582)
                     /locus_tag="PK754_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB08907.1"
                     /translation="PLSQVLINLINNAAQASPADGKVTIQTRQENTTVRVVITDNGAG
                     IPKDIIDKIFDPGFTTKPESEGTGFGLSISAEIIKKHNGRIEVVSAPGMGSTFTVYLP
                     VDPRNVS"
BASE COUNT         4016 a         3032 c         3439 g         4095 t
ORIGIN      
        1 acgctcttca aaatcaggat attatcgcaa ttgttccgat gcgaagccag gatcgcacaa
       61 aaggtgttct gcttctcggt cctaaaattt ccggaaaagg atttactaaa gaagaattag
      121 attttgtttt tacgctgggt aatcaggcga tgatttcgct tgaaaacgca tggcttttcg
      181 aagattcact ggagcgtaaa cgactggaag aggaactcga acttgcacgc aaaattcaga
      241 tgggcttgtt acctaaagat tttccaaaac tgaacaattt tgaagtgtac ggtttgaata
      301 tttcatccaa acaagtcggc ggcgattact ttgatgtgct caaaatcaat gaaacacaat
      361 atatcatagc gatcgcggat gtgagcggta aaggcgtacc ggcttcgctt ttgatgagca
      421 atgttcaagc cagtttgctg gcgctcagta ccgaggtcag tgattttagc aagcttgtct
      481 ataaggtaaa taatatcatt tacgcgaata ccgattctga aaaatttatc acgttttttg
      541 gcggtgtact ggatatcgaa aaacgtacgt ttacatatgt caatgcaggg cacaatccgc
      601 cgtattggcg gaaaacggat gggacgttac atacactgga taaaggcggt ttacttttgg
      661 gtatgttgcc tgacatgccg tatgaacaag aaacgatcac gctttcaccc ggtgaaggca
      721 tcgtcatgtt taccgacggc gtgacggagg cgatgaacga aaagctggat caatatgacg
      781 aatcgcgcct tgaagcattg atcaaaacgc atccgaagat aacggccaaa gaatgtacga
      841 aaaaaattgc cgctgaagtc aaaacctttg ccggtgcggc gccgcagtcg gacgatatca
      901 ccgttgttat cctccatgct atttaagagt ctacctttat tttggaaaca tagcggcgtg
      961 atgtaaaaca tccgccgttt ttgatatgaa agtgatatga ataaaatgtt agtaaagaat
     1021 ttattttttt tagcgttgtt tgttaccgtc gctgtagtca atagccaaga gaccggtgtg
     1081 atcacgggcg tggtcattga cgcgaaaacc aaagagtatc ttgccggagt gaatgtgcag
     1141 atactgggca caacgcgcgg aacggtagcc gatatcaacg ggcagtatcg gttggataaa
     1201 ttggctgacg ggacataccg cctgcaattt tcactgatcg ggtatgcgcc gctcatcaag
     1261 accgatgtca ttgtcacggg gaacaagcca tttcggctca atgttgcaat ggaagcggaa
     1321 agtatcgaag gcgatgaagt gatcgtaacg gcgggatatt ttgaagagca aaatcaaact
     1381 aagacgagca cggctaatct atcccgtgaa gaaatacggc gatttcccgg cggttttgaa
     1441 gacgtcgtgc aaaccgtggc tacactgccc ggtgtggcgg tcaatggcgg cggtggacgc
     1501 aatgatttac tggtgcgcgg cggcggaccg tcagaaaatt tgtatatcgt caacggattg
     1561 gaaattccca atatcaatca ctttggcacg cagggaaaca atggcggttc gctgagcttc
     1621 attaatctgg atttcgtgga taatgttgat ttttcgacag gtggtttttc ggcgcggtac
     1681 ggtgacaaga tgtcatccgt cacacaactt acattgcgaa gcggacgcga tgatcgtatc
     1741 ggaggtaaag ggctggtttc ggcgacgcaa tttggcgtca acaccgaagg accattggga
     1801 aaaagaggaa actttatcat gtccgcccga cggagttatt tggattttat tttcaaagcc
     1861 gccggttttg cttttgtgcc gaggtacacg gattttaata tgatcggaca atacgaacca
     1921 tcgccgcgtg ataaattcac ttttctcgca ttgacggcgc ttgatgatgt gagtaaaaat
     1981 aatgaaaaac ggaaagatcg tatttttaat tccgggcttc ttgataatga acaattcata
     2041 caagttgttg gtgccggtta ccagcacaac cttgaaaaag gattttggca ggttacggcc
     2101 aatgtcaatt tttcaggata tcgtttttca caaaaagata cgacgctacg cgaatattat
     2161 aacagccgtg ccaatgaagt ggaaacgaat gcttccgttc agcggtattt tgtgattaat
     2221 aaatccatgg gtattctgac cggattgagt atgaaattcg tcaataatcg caatgaaacc
     2281 gtatttggtg atacgatcgt ggatcgcaat ggtaatcgta ttcccgcgga ttcgctgggc
     2341 ctggcgggat tggatgaaac acgtgcgtca gcgtataaat acggcggatt tacggaaatg
     2401 gattgggtcg tatcgccgcg tctcaatgcc aatgcaggtt tacgcgtaga tcggtactcg
     2461 gctttgaaaa aaacaacgta tgttgctccg cgtttggcat tcaaatataa aatatcggac
     2521 aaatccaatc ttaagttgag cggcggaacc tattatcaat cgccatcgta cgtatggtta
     2581 gtcaatccgg ctaataaaaa actcaaagcg ttgcgcaacg acatggtcgt agcgggtgcg
     2641 gaccgtttac tgcgtccgga tgtgaaactc tcaatagaaa cgtattacaa aaagtacagt
     2701 catctgccga ccggaacgca aaaaggtatc aatgattata tcgtcattac cagcaccggc
     2761 accgggtttg gaggacgtga agataatttt caatccttcg gatatgcgac gttggtttcg
     2821 cgtgctaccg gaaaagcgtt tggcgtcgaa atgttactcc aaaagaaatt ttctgacata
     2881 ccatgttacg gtcaggtcag tattacggtg ggtaaaagta cagtggtagc gggcaacggt
     2941 aaaacctatc ccaatccgtt cgatcagcgc tttattttaa atatttccgg cggttataaa
     3001 ctgaatacca aatgggaatt cagcgggaag tttcgctatt ttaccggcgc tccgtataca
     3061 ccggtttatc gtcctcagga taatcccgtg cagccgggtg tcgttcaaaa tcttccggat
     3121 gagtaccttt ccaaacgcct gggggcgagc catcatctgg acttacgcgc ggatcggtat
     3181 tttaattatg aacattggcg gcttattatt tttatggatg tgcaaaatgt atataattac
     3241 aaagtcaaaa cgccgccgtc atatgatttt gcagaggata aagtgacccg tggtgatcgc
     3301 ataggaatac tgcccacgat cggtatcagt gctgaatttt aatctaacgg agaaggatat
     3361 gccgaaaata ttgatttgcg taatcgccct gaacctaacc ggttgcggtt tttttatcac
     3421 gccccgctcg tcagatcagg tatccattcg cgaggaaaat ttagtggctc atttgacgta
     3481 tctcgcatcg gacgaattag aaggacggga ggcgggtaca cgcggagaaa aattagcggc
     3541 cggttatatc gcttcgcagt taaggcaaaa cggtgttaaa ccttttgcgc tagcccgaac
     3601 ggcagacggc cgagacagcc tgtcgtattt tcaggaattt tcttcgacta aaattgcgat
     3661 cggcaaacat tcatccgtga ttttttatga cagcgcaagc gggaattcgt tcaccatgcc
     3721 ttatgctgaa acatttagta attttcatga atatgtagat aattgtaacg tttctgcgcc
     3781 gctcgttttt gccgggtttg gtatcacggc gccggaatac ggatacgatg attatgcagg
     3841 actggatgta cgcggtaaag tcgtattagt gcttgatggc gaaccgacat cggaggatga
     3901 tcagtttttc gacgggaaaa tagtaagttc gtatagcagc gctttatttt ataaaaggca
     3961 tcgggccaag gaattgggtg cagcggcgtt gtttgtatta tcatatcaag cgcttttgga
     4021 aaaatgggat gagtatgtgg atttcttcgg atccagccgt atgatttttg atccatcgct
     4081 tttaccgaaa tcggagggcg aacgggttcc tttttattac tgtcatacgg tatttgccaa
     4141 acgtgcattg gcatccgccg agacgggttt tgagcaaatt caatctaagg cacagtccgg
     4201 tgcgatggtg ccgcgtttta ccgtgaaaaa tatacgttgc gatgtgcgta tcgaacgcac
     4261 ggttacacaa gtgaaagcga tgaatattat cggtaaaatc gaaggtcatg atccggtaat
     4321 gaaaaatgaa tatgtggcta tcggagcgca ttttgatcat ttgggagtca atgcgtcggg
     4381 cgccatattt aacggagcgg atgatgacgg ttccggtaca gccgctgttt tggaaacggt
     4441 tcgttcggta tttgcttcac aacaaaataa acgttcggta ttggttgtat ttcacggggc
     4501 ggaagaaaaa gggctgattg gttcaaagta tctgaccgat ccggccaccg tcaacgcgtg
     4561 gacatggaaa gacgtcacgg cgcaaattaa tatggatatg gtaggaaggg aaagcgccga
     4621 tagtatttat gtgatcgggt cgggccgcct tagttcacaa ctccaatcaa ccaatgaaac
     4681 tattaatcga ggaacgggct acttttattt tgattacaca ttcgatcgtg acgatgatcc
     4741 caatcaatat tattaccgta gcgatcatta taattatgcc aaattaggta ttcccattgt
     4801 ttttttcttt gacggcatga cgtccgatta tcacaaaccc agcgatgatg ttgctaagat
     4861 caatttcaaa aaattataca aaatcaccga tcatacgcgc cggctattga tgaatctgtc
     4921 caatagaccg gagcggcttg tcgtggataa acactaagcg atgtttctcc gtcgtaagga
     4981 aataaatata tacgaatttc tgcgccccgg atcgcgcggg cacggatatg ttgttaattt
     5041 tcatcgtaaa tacttcgatg tatttcgcgg tacagacttg gaaaatcagg atgcattcat
     5101 caatgaggta tttattggtc tgtcttctat tcgcgaagag agcattcgcg aaaaagccga
     5161 tcattatctg atgaaagcgg tttattttcg ttgctggcgt ttgttcgaag acaacaaagc
     5221 atacaaacag tccgaagtac cggcgagccg tgcacaacgc gatgatgaag atgatgatgc
     5281 cgtaacgcgt gcaccctcta cgaccccact acctgattca cctcttttag ctcaagattt
     5341 gcttgaacat atccatcgtt ttcgtatgac actaaatccg cgagagatcg tggttctcaa
     5401 tggattgatc gacgaggttt ccatcgctga aatcgctcga aaagcggggg tcactgcaaa
     5461 ctatgccggc gtactgatac acagggtacg tgagtcgctt catgtgtatt tagaagacat
     5521 cgggtatttc aaaaaaaata aaaaaaagtt gtaagtgttt tgttggtatt cgtacttact
     5581 gtttgtgagg agttagctta gtgcaaaaaa ggccgtatta tgacagatga tgaaaaaaaa
     5641 ataaatctag attttttgga cccgttacgt cggagtccgt ccgtgatatg gaatgccgag
     5701 atgatgagtc gttgtattca cgggtactcc gttatgagta aacatgaacg ggattggttt
     5761 gatacgcaga ttcgtgaaga agattccgcc cgtaatcttt ttgaacaagt atttgatgat
     5821 gatattcttt tggatccctc ctatcgcaca gaatctccgc tcatacaaaa aatgagcaaa
     5881 ggaatccaaa tacgctgtgc gacgggactc acattgcaaa tagatcaatc catggcgttg
     5941 atttctgaag cgggtccggc gtggatcggt caaagtgcat taattaagtc ggtagggtgg
     6001 tccttacgaa ttcttgaagt acaaacaggg ttacggttgc catgcgcatt gctatctgaa
     6061 caattttcta gtgaacttaa agtttgtagt gttccgcttg ccgataaaga agattcagtt
     6121 cacacagata ggcctgtatg gtatatttgg tcagccgctg caacgattgt cgtgatgatc
     6181 atggccggta tgtggtggct gcagaagccg actgaagttt taacgacgga tacgaagctt
     6241 gatatttcca aagatacact cgtggtgcct caacgtaatt tcacggaaca accccaagtc
     6301 gagttacctc tgcgtcaagt accaaagccg aacaaagaaa aattattggc tgcacatttc
     6361 gaaccgaacg ccgttcttga aaattttatc gggcgtacat accgcgcgga ggtacgtata
     6421 gaaataatcc agccctcgtc tggggatacg cttcgcgggc ctttggtttt tacttggaaa
     6481 aaatcggatc ctaatgtaaa tgttacggta aatattttaa acaaccgtaa cgcatcaata
     6541 tgggaagcta atacgaaaga agatcaatta tcatataacg gtgtattggc accgggattg
     6601 tattattgga ttatcaagtc cggtgatgtg cgtcttgacg tgcgaaaatt ttatatccct
     6661 attcagtaac cgatcaaaac aaatgcaccc caatctccgg ggtaggatgt gtgttgttgt
     6721 gctatccatc gtttttgtgt tttttgaagc gccagactca ggtttttttc agtaaacaca
     6781 ttacgataaa actctgtcat aaattccgcc gttaagcggt catctaccgg ccagagtgaa
     6841 acaagcacgt gtcttgctcc ggcgtaaata aatccccgcg ttaatccgat caccccttcg
     6901 cccttctcga tttttcccag cgccgtttta caggcactga gtgttaccaa gtgtgcattg
     6961 aggcgaaggt tgtagatttc tcctgcgtat aaaacatctt ctttcacgtc aaccgagtca
     7021 tcggagtaaa gagctatgcc tgataatttc ggatttgctt catttataaa accgtgactg
     7081 gctaagtgta taaacccata atttgtaaga ctgtctgatg taagaacgga tttaaccgct
     7141 ttttgatgag ttacgatttt ggagttgaat ttccgattcg aaaataattc ttgaatatta
     7201 gtaacttcag tttgactatg aggaagttga gcgaagtgag tgcctctggt aaaaaaaccg
     7261 cgatgtgatg acatcgttga atcatcaaat tgatattcct taatgtaacc gcttattgtc
     7321 gtatcggaaa aaacaggtgc gaatccaaaa aaagcattgg ttggtggttt gtcggaagtt
     7381 gtaatgatac gatccgcaga aaaaacatat tgaattggga aatcttggca taggtatcgc
     7441 actttcgaag ttaaattcga agaaacagaa gaagttacta aagtttcaaa tggcaggtaa
     7501 gataaactac catccggaat gataactaaa gttgccccta gtttaacgga ttgaagtgaa
     7561 ggttgcaccc atcgtttgta aaaagaatat gaagactgcg aaagttcatc tgacgcaaga
     7621 ttatttacat gtttacgaat aatacgaacg gatgattcta acgttgtatc attatggaga
     7681 acttgcatag agatacgcgt ggctgtaacc gtgatcactg ttgttataga atcgccggta
     7741 aaataatcaa ttaatacggc ggcatctttc ttgagggtat gttggatttc ggctatagtt
     7801 ggtattgttg atttgtattt cagtttaaaa tattcgggat tgttttgttc gatccaattt
     7861 tgaagcgaat cgtattgagc tttttgataa aatataattc tctcaagacg ttgtatttcg
     7921 ctatgcttgg aactgtcgtg aagttgaagt tgattttgga gttgagtttc gtacgaagct
     7981 aagttgattt ttatatcgcg ttctagatta cttacggaat cgggcatttg tgtgaaatta
     8041 cgaatttgaa gatcatgcaa agcttcgcgc aaagcacctg atttgctgcg ttgggacagt
     8101 gtaaaggcgc gttcaatcca caacggtgct tgcgttgcac ggtaggctgt aacgcaagcc
     8161 cggatacctt cttcataaat tcgtttagcc ttggtgccta gaaaaatttt tgacccttcg
     8221 aggcggtatc cttgacggag atagtcaatc caccccgata ccaactcgaa tagtttgaac
     8281 gactcttcca tatgtcgaac atcgtgcttt gttagaaaaa gttgatgcca tagccgagct
     8341 ttttgtgcta aaatttcaat gcttacactt tctagtgttg catcttttaa cgtaggattg
     8401 gatagaatat tactatcgtt aaatccaata agagaggcat ttaacgctgc ttggcttaaa
     8461 cgaatagccg ctgtgatgtc gttagttttt tttgctaagg atgcgtggcg agataaaatt
     8521 tctgccacca atggattttt aaagccaagt acatccttgg ccatattcaa tcccagagta
     8581 aataaagaat cagcttctct ccaatgttct ctcctttcgg caatatctcc gaaataaaca
     8641 tatccttttg cgacctcaga gtgcgatccg tattcacgct tgttaatcgt aaaagctagt
     8701 tgatagtaat attcggctga atcaatttgt tttaattctc tgaaaacatt tccaatgttg
     8761 ttataattta ttgcaaggat aacactggat ggatcatgac gttttaggcg tattacaaga
     8821 gccttttttt catattcgag agattcatgg aacatacctt gattataata gcaaattgca
     8881 atgccaatat acatgtccga tacatctggg tgttctggtc ctagcgtgct gagtgcgaca
     8941 tcaatggctt tacgataaaa catcattgca cgctcgtaat cgcccatagc gtcataacaa
     9001 attccaatat taaaatatat aatagccgtt cgcggatgtt tttcaccgat tgttttaatg
     9061 cgtatattta aagcctttaa atataactct tccgcagttt tataatctcc gcgtatgcga
     9121 taaataaccc ccatgttaat gtacgaagac gctatcgaag aatgattttc gcccagcgct
     9181 tttattcgaa ttttgaggcc tttttcataa tgagagagcg cttcttcata atatccttta
     9241 tctttgtaag caagccctat attagtatga accgccccaa catcctgatg atcaggtccc
     9301 aataatgctg tccgaagttt caagtttcga cgattgatat ttaatgatga atcataaagg
     9361 cctcggctcc acaaaacagt ggcatatgta ttgagtagct ctccataaac aatatttgaa
     9421 tcgcctaatt tacttttaac gagtgcaaga ccttctgtag ctgtagctgt agcgtccatg
     9481 aagaatccgc ttatacgttg cccataagtg atctcatcat atacagttcc agcttttttc
     9541 caattttctg tttttatata gagttttaaa gcgtctttga ggaaaacgat agcgctatca
     9601 tatttaagcc ctttaaggta ggtttgtgct gtggcgtgac tctgggctgc cttaaatgaa
     9661 tcattttccg tttgcgactg taacgtaaaa gttataatga atccaaaaat aaatatttga
     9721 aaacttaatt tactcatacc ccctcatttg taaatagtag cgctctttct tccgaccaat
     9781 aataaccttt tttagaagat gcaggaaaac cgacatgtcc tcccgtccga ggcatttcta
     9841 aggtaatgtg agtgtgatgc tgcacctgtg tgataggaaa gcacgacggg cctaaaaatg
     9901 gatcgttttg tgcattgacc agcaatgtcg gtatcgtaat tttatccaaa aacctcaccg
     9961 cactgcaccg ggaccaataa tcttcagcgt cgcgaaaacc atgtagcggt gccgtatagc
    10021 ggtcatcaaa ttggcgaaat gttttaattt ctgaaaatcc tgtgtcactg atttgtccgg
    10081 gaaacatcgt tgatttcaat tttatttttt catgaagcat gcgcaaaaaa cgacgcatgt
    10141 agatgcggca ggcaggagac tccattgtta atgccgatga cttgaggtcc acagggactg
    10201 aaaatgtgac cgctttacgg atagccggat ggacttgatt tcccttttca ccgagatatt
    10261 tgagggtcat gtttccaccc aaactaaatc cgatcaatac aatttccgta tatcctcggc
    10321 tgagtccatg atgtatgacg acttctaaat cttcggtggc tccgctgtgg tatgaacgca
    10381 gcaaacgatt cgattcaccg ctgcatccgc gataattcca ggcgagaata ttccaacctt
    10441 cttcatgaaa tcgtttggcc atgccgcgca cgtaagcgcg cgaggtattc ccctcaagac
    10501 catgcgatag gattaccagt ttggatgatg cgttcatagt gagccaatcc aaatccagaa
    10561 aatcatcatc cggtgtcgtt atgcgttcac gcgtaaatga aacgtcatgt actttccgaa
    10621 ataacgtcgg aaaaatcgtt tgcacatggc cgttaaaaag aagtaatggc gcccggtatg
    10681 tcgatgtttc gattaaaggc atagtccttc tataaattac gtttccaaaa ttcaatcatg
    10741 gttttgcata gatgcagttt ggccgctgta tccgtaaacg tatgtttgcg catggggtac
    10801 atcatcatgt caaaacgttt atccgcgcga atcagctcgt caacaaagtg ccatgtattt
    10861 tgcggatgga cattatcgtc atacgtgcca tgcactagga gtaatcttcc gctcaggttt
    10921 ttcgcatacc ggttgagatc tgttttaaga taaccatccg gattttcctg aggggtcttc
    10981 attgtagctt caccatattt ggtatcataa taatgccaac tagtgagcgg agcgacggcg
    11041 acaccggcct taaaaagcga cgttcgtgtc atggccagta gagtaaaggt tccgccacca
    11101 ctgcgacccc atatgccaat acgttcgggg tcaacgaatg atcgcgtttt aatccaagcc
    11161 acggcatcct ctagatcgcg taattcaccg tcgctggctt gcatgcggac aacggtattt
    11221 tccagtattt tgctgattcc ggtagcgctt cgattatcca cggtgatgat gatatatccg
    11281 tcgcgcaccc ataactgatg cggcatgatg gtctctgtcg gccagcggtg attgacttgc
    11341 ggtgcgctcg ggccgccgta cacatggaga atgacgggat attttttggt cgaatcaaaa
    11401 tcggcaggac gcagcatcat tgcgggcatc ggaaaactgt cacgcgtggc gatcgtaaaa
    11461 aaacggtatg gtacgaatcc caaagtacgc gcatacgtcg tatccgccgc ggcgatcact
    11521 tgtttgatgg agccgtccgg tttgtgcaac gtaagggaag gcggtgtatc ggcggatgaa
    11581 tgatggtaca ttagaaaccg tccgtccgga cttagtgtcg catcatacgt accatctttg
    11641 atcgaaagtc gttttttatt ttttccgtca aaacgaatgc tgtaaatgtg gcgttcaatc
    11701 gaagattttt ctaatgactg ataatataag gtctgcgttt tttcatctat accgaccaca
    11761 ccgcctttgc tgtacagcgt tgtcatttgt acagaccatg ttccggacgt aacggggcgt
    11821 atcaagcgcc cgttactttg ataatgatac agatgcgtgt atccgctgcg ttgagacgac
    11881 cagataaatt cgctgccgcc ttttaaaaaa tacaaatcgt tgtgttcgcg gacaaatccg
    11941 gagtttattt ctgtaagtat ccgtgtaggt cttccggaag ccgcatctac aaaataaaga
    12001 ttcaattcgg tttgcgaacg gttcatcgtc tgcatggcca atcgcttgtt gtcgggaagc
    12061 cattttaccc gcacgatgta ttcatacgtc aggccggtca tatccacagc caccgttttt
    12121 ccggtattca tgtttgtgca gtgcagggta gcgataggat tgacgcttcc ggttttagga
    12181 taacgttggc gtatcaatgc cggaacggcg ggtttataat cctgaaaaac cacttcgtca
    12241 acttgcgatt catcaaattt ataaaacgca agtagcgaac cgtcgtctga ccaaaagctg
    12301 ccaagctctt cgcgatcata gatctcttcc caataaaccc aggacaaacg accgtttagt
    12361 aatgtcgaac ttccgtcgga ggtgatctgt gtttcttttt gcgaaacaag atcgtaacta
    12421 aacaaatcat tgtggcggac gtaaacgact ttttttccgt ccggagcaag ccggggcgcc
    12481 atttcttctt ccgatgtcgc ggttacgcgc gtccattgtg atgttttaaa gtgaaagata
    12541 aatacgtcgc cttcaaaaga aaataaagcc agcgaccctg atgcatccaa accctccggc
    12601 caaggcagtg cggcgggaac ggaggagata tgtttttgta aacccgcgat agccgtacgc
    12661 atatcaaaga gtttaaagcg ttccccgctt tgggggtcca gcatttgata agaacggtcg
    12721 ggccatgcga cgcgtgtgtc gtaaagtata atggtattct gcgaagtcca aaaataagga
    12781 ggcgcttccg tgcgttgttt aatttcaccg ttgtacagat attcaggcgt gagtgttacg
    12841 ttcgtttgag cgtaaacggg cacggttatc atcaccgcta ttatccataa taatcttttt
    12901 ttcatacatc actttgccgg tattatgggt tgttatgcat tgttgggatt cattcgaagc
    12961 tggattgcgt gcaatttttt tccggtacga tacccgtgaa ttcccatagc cggtccgatc
    13021 agccaatacg ttaggccggc ccaattggcc atagcggagc cgccgtataa aagtaaagag
    13081 gttgaaaaaa gcgcaatacc gatattgatg atatacgcac gccgttcgga tcgggcaatg
    13141 aaaacctctg cttcattgag ttcaaatcgc gcgcgaagac ggtacgcatg gtggtacagc
    13201 aaggcataga taccgcacac ggcgatgtat cctgcattgt agatgtacat catgtcgtcg
    13261 ccttgacccg ccgcgatcgg cggctgttca taagctacgc ccaacatcat attgacgacg
    13321 agggtcatga caaatttgag gggataaacg aagaataaca ctacaaaaag gagcgcggca
    13381 ttccaaacaa tcgtaaaacg atcttgtaaa ccgaaacgcc tgaaaaattt gtaatgccaa
    13441 taccataaca tcaccaagat cgtaaaacac gtagcaaatg ccgggaaacc gcgcatggtt
    13501 tccaaaagct gggaaaggtt tttgggtact tcgagtgaaa cgaccagcaa agtcattgca
    13561 aaagcaaata ccgcatcgct gaaagcttcc acacgggtga cttcatgccc gcgccagcga
    13621 aaatgagggt caagcgcttg attactcagc ataacgacct cgctaatttc gaagttaatc
    13681 tggaaaaaga agaaacgtta aagattgttt tgcattacgg cagtttttcg cgccatacgg
    13741 agtacagatt cttctgtcgt ataatcggca atgcgatcca acgcaatcca agctaaatct
    13801 ttggactcgg aagaaatgcg cagcggtatg gcatcatcta tttcgatcag aaaccgcacg
    13861 tcgtaatgaa gatgcgccgc gtccgatttg tgcggcggaa tttcgtgaat atccaaatca
    13921 aagggcatat cggacaaaag cgttgtcgct tcgacaccgg attcttcctg cgcttcgcgc
    13981 aaggccacgc gatctacatg gggatcgcca tccgcatgcc cgcctaattg cagccatcgg
    14041 ttgagtttgc cgtgatgcgt cagtaaaact ttatcgcgcg aggggttgac gatccatgcg
    14101 ctgccggtta tgtgcccgat ggacagataa cgctcaaaac agtcggggta tgtttgaaca
    14161 aaatccaaca tacgatcacg catggctgat tcgtgcggat cgctcggatg gtatgcgttt
    14221 aatcgggaga tcaaccagtt gcggtgcatg gcttaagaga cgttgcgcgg atcaacgggc
    14281 agataaacag taaatgtcga gcccataccg ggagctgaca cgacttcgat ccggccattg
    14341 tgctttttga tgatttcagc agagatagaa agcccgaaac cggttccttc gctttccggt
    14401 ttggtcgtaa atccaggatc aaaaatctta tcgatgatgt ctttaggtat gccggcgccg
    14461 ttatcggtga taacaacacg aaccgtggta ttttcttggc gggtttgtat ggtgactttg
    14521 ccgtccgccg gtgaggcttg cgccgcatta ttgataagat tgatcaatac ttgggatagc
    14581 gg
//
LOCUS       DAOQNQ010000050        14536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1476_length_14536_cov_6.363856, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000050 DAOQNQ010000000
VERSION     DAOQNQ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14536
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1476_length_14536_cov_6.363856"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2184)
                     /locus_tag="PK754_05080"
     CDS             complement(<1..2184)
                     /locus_tag="PK754_05080"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011336150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNB08908.1"
                     /translation="MDASIPQNFHPAIARWFVREYRQPTEVQSQAWEAIVEKKHTLIA
                     APTGSGKTLAGFLAIVNDLVIEGLERGLPQETRIVYVSPLKALSNDIEKNLQRPLQAI
                     QDELESLRLPRVEIRVQVRTGDTPAGERTAMLKSPPHILVTTPESLYVLLTSDGGRKM
                     LQSARIAIVDEIHALVGSKRGSHLALSLERLQSLVSRPLTRIGISATQKPLTQVADFL
                     TGRLEPGAAPCTIIDVGHRRAMDLGIEIPRSPLGAVMEGEVWDEIYERLVELISEHRT
                     TLIFVNTRRLAERLSFQLIERLGADVVRAHHGSMSKEHRLDAEQRLKSGSLRALIATA
                     SLELGIDIGSVDLVCQISSPRSIAAFLQRVGRSGHSIHGTPKGRLFPLTRDELVECTA
                     LLDAVHRGELDRILMPEQPIDILAQQIIAETSCRECTEDELFALSQKAYPFRNLKRET
                     FDEILTMLSEGFTTRRGRRGAYLHHDMVHRVVRARPAARLVALTSGGAIPDNFEYDVR
                     LEPTDTFIGTLNEDFAIESSAGDIFQLANHSWKITRVNDGVVRVVDAGQAPPSIPFWL
                     GEAPGRTIELSQAVARLREKIGEQLGSVEMLKPQVLSASVRQNPDGPVVPGKTPWMQP
                     AVQWLCSELGLGLGAAEQLVHYVASGYLALEAMPTQDTIILERFFDEAGDMHLVIHAP
                     FGNRMNRAWGLALRKKFCRKFNFELQAAATDDAIVLSLGSTHSFPI"
     gene            complement(2244..2849)
                     /locus_tag="PK754_05085"
     CDS             complement(2244..2849)
                     /locus_tag="PK754_05085"
                     /inference="COORDINATES: protein motif:HMM:NF018984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HNB08909.1"
                     /translation="MKNASPSFIFLGGHTVMDFLNTVYLQKNSTVETLQHNKDILVWM
                     EEAGILTHARRNELNRIWIHSPESEAVLRKVHKFRRVCFEVLSCIMAHKPVPKTATDE
                     INFILSSGGMVLRLEESPSGFKKQAVYAFQSPEHFVVPLAVSLVDLLESGDPSRIKQC
                     ANPRCRLFFYDQGKNKTRRWCSMKTCGNRRKSANVYARKRA"
     gene            complement(3389..4339)
                     /locus_tag="PK754_05090"
     CDS             complement(3389..4339)
                     /locus_tag="PK754_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08910.1"
                     /translation="MSKLVASIVLLLSSFGCTDHKAKPYEGRLDKDYELVVAHVAPDE
                     DREFPIEVFVGRLHTIFPDSTDAANLQDHMMRIQLRAFVDEYQALLTYHDDAEVYITA
                     GDQTVRLEHVGKGTYRDVADALHVYGQQTCALLVKRSGGKTYQGIVKVPGDITINNIS
                     VGDTVLSYPKKETPQSSICYGETFVSGSVVTDGYVYRFRMPYLYGNEEDIVQLWSSNR
                     AAAPIIISKCGGISYFSIPWTSMALDTNLTRAWGMRETTSIEPAVWDSMRYWRYYAPI
                     TYRSNITDEDGDKIAGYFGAYNSVKTNFVVKVLRDSCECE"
     gene            complement(4356..5327)
                     /locus_tag="PK754_05095"
     CDS             complement(4356..5327)
                     /locus_tag="PK754_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08911.1"
                     /translation="MKNLFLVFIQSVVVFSGCIGCLDEHDEKPSLRRKLDKDYEFVIG
                     HVMPDEDRTYPIEVFVGRLLVPRPDTLNNASIQEPLLKIQLRQFQNEYLATLSYHDDA
                     QVFITAGDQTVELQYVGRGTYRDIANALHISNLQTCSLKVIRPKGKTYQAFAKVPGSI
                     SLSSVQGGDTIVSYLKRRKSSQLCFGSPRIIGPQVDGALFYRSQQVDNSHNFDERIVF
                     LEFINSFGCPHTLNNCEEASYANNIWWTSTAFDTVLSHAWGLGETTGARDDMIDTVSY
                     WKYDAPITDRSNITDQKGKKIAGYFGSYNSVSINFVVKAMNDSCTCD"
     gene            complement(5405..6562)
                     /locus_tag="PK754_05100"
     CDS             complement(5405..6562)
                     /locus_tag="PK754_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08912.1"
                     /translation="MKTAKWKIVPICLFMVWTMACQEKANEDKGAQYDNDVSLTREAL
                     NEIASILAADPSWITQETITKLSKSSIRLNELPSSSVLSKTSHYNFPVEIELYSKSQA
                     NVMAKGATSTTYYVGLDPDVYHFPEDVQEEETGLPVIEEPAFYYDGNSITETTITIDP
                     KNPTNETIFFIKESNDDQINLAKSASTPIAGTYMAFKKIDLKNKEDASDEEFEVYFSY
                     NDPNSTTIASTTTHIFDGGNHLDAAEVYRYYLDINDKEAYEVDPNEEIALVRVDNLST
                     PLRMVAIEADQSNHTYEQGKYNRNEGANVVVYTQTDFYDMADPTSMVTQNQGYKVDVY
                     NSVNDDRYQHGGIRNITAANLAQRTNDGESSFNTVGVGLTDMDWWMIQLVY"
     assembly_gap    6834..6933
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6934..7114
                     /locus_tag="PK754_05105"
     CDS             <6934..7114
                     /locus_tag="PK754_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08913.1"
                     /translation="GLDGLDGLDGLDGLDGLDGLDIPYNTHNISYDKNITKKAAEICR
                     YFTENLVGNLPTRFR"
     gene            7446..7787
                     /locus_tag="PK754_05110"
     CDS             7446..7787
                     /locus_tag="PK754_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08914.1"
                     /translation="METSSLFTIIAAFLSQSPIVVVWIVGAVLAYRQKPVYPRKSLLI
                     IIAMLLFLGQLFINTILNIVFPMMWDTQQLLLSYNVKAMVTSMIMTAGWILIMMALFM
                     KEKSRDGFGQN"
     gene            complement(7794..8573)
                     /gene="truA"
                     /locus_tag="PK754_05115"
     CDS             complement(7794..8573)
                     /gene="truA"
                     /locus_tag="PK754_05115"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNB08915.1"
                     /translation="MPRYKLTLEYEGTRYSGWQVQKDTHTVQGTLMYAITEELRNKNF
                     DFQGAGRTDSGVHALHQVAHLDCTTVLDPYKFRLAVNDRLPHDIHIKQIEKAPGSFHA
                     RHDAVARSYLYQISRRRSAFGKRFVWWIKDELDLARMQEASRFFVGLKDFKNFAAKDG
                     EADSTKVNLEELRIESRGDLILIRVVGSHFLWKMVRQITGILAEVGRGNMKPGDIAGL
                     FRQTSPSLARLTAPPSGLFLERVYYKNDERLQTLEPFLAIR"
     gene            complement(8775..9779)
                     /locus_tag="PK754_05120"
     CDS             complement(8775..9779)
                     /locus_tag="PK754_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011733952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNB08916.1"
                     /translation="MKKKEFEDILNKLAQQLTYEAQKNVFSSSKVFENRVRDILKTLI
                     TDSKVEIDYNPHPYAFPDIAVGEYGIEVKFTEKDNWRSVANSVFETFRNKEVVFIYVV
                     FGKMGGLPEVKWQSYQECVIHVRTSHVPRFELEIDAKSSLFEQMKISYDDFSVLPIEK
                     KMRYIREYAKARLKEGERLWWIEEKPEQEHTLPIQVRLYTELSQSEKRKLRAEASLLC
                     PKIVAGSRVKNKYNDVAMYLLTYHGVLASQARDLFSAGSVAMASDSIRGGLYIHRALS
                     DIEDEMINAAKTMEDALFVEYWKESVPPNKRIKRWLELADRNATGWKPSEVLFLNKKL
                     "
     gene            complement(9781..10740)
                     /locus_tag="PK754_05125"
     CDS             complement(9781..10740)
                     /locus_tag="PK754_05125"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009647144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HNB08917.1"
                     /translation="MQRRVSQSILKKKKKFSIVSLFSGCGGMDLGFIGGFSIFGNEYY
                     KNPFEIKWANELNPYACKSYSRNIDSNIHCGDIRDYIGKLPSQADVLIGGFPCQDISI
                     NGKGLGVNGKRSSLYTYMIEAIKLTNPKVFVAENVKGLLMKTRRESLNKILHDFNELG
                     YNISYQLYHSADYGVPQSRERVFIVGTAKDVKSFVPPKTVRQKENWLTSYDAIHDLEK
                     VNENEQINHIWSLAKKSGEQGGRRLEPSKPAFTIRAECHGNIQFHYSLPRRLSMREAA
                     RIQSFPDNFIFEAKLRETERQIGNAVPPVLAWHIAQSVKDCLL"
     gene            complement(10838..11149)
                     /locus_tag="PK754_05130"
     CDS             complement(10838..11149)
                     /locus_tag="PK754_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005805154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNB08918.1"
                     /translation="MQNETFGDTIRKLREESGLPLRKVAAHLDIDPSTLGKIERNERN
                     ANKEIIEKLAELFSVNSKPLLVSFLSDKVAYEIMEEDYTKDILKIAEEKVKYYKKQRH
                     N"
     gene            complement(11559..12329)
                     /locus_tag="PK754_05135"
     CDS             complement(11559..12329)
                     /locus_tag="PK754_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HNB08919.1"
                     /translation="MMLMQGKQGLICGVANKRSIAHAIAKSLAREGAKLAFTYQGERL
                     KRNVEEIATELGSNLLYDCDVTKDDDIKRLFEAVSKDMPHIDFLVHSIAYAQKDDLEK
                     PFSTTSREGFRVAHDISAYSLIGMAREAMPLMKEKGGAIVALTYYGAEKVIPGYNVMG
                     VAKASLEASVRYLAADLGPSNIRVNAISAGPINTLAARGISGFTKILDITPQKAPLRR
                     NVEVDEVGDASLFMVSPLSRGITGEIMYVDCGYNITGM"
     gene            complement(12452..13906)
                     /locus_tag="PK754_05140"
     CDS             complement(12452..13906)
                     /locus_tag="PK754_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HNB08920.1"
                     /translation="MPIEIPEALKEYLKLFPGEYADAFSQGLDFERRTALRVNTLKFN
                     KTDFIHRLAQQNFRFTEIPSMPDALLVEGEDARISKTIEHFLGLFYIQSIASMLPPIV
                     LNPTASDFVLDVSAAPGSKTTQMAQMMKNGGCMVANDRDDKRIKTLSHNLDRLGVINT
                     AMVNMQGERIGNLVPGSFTKVLVDAPCSALGVIHKAPSAVSNLRHLNKFAFIQEQLVV
                     SGLKALRSGGTLVYSTCTISAEENEGLVDTILKRYPSLHLEAFDLPEGIAALPGITAL
                     GEKIFDPRVALTRRVMPSAINPEGFFLAKFRLDEAIEVRTGKTAQAEHKKMYALDGSS
                     HAEIRGMGKYFSGLFGFDENFWDQFVYHIKDDETFIASEDWRGRESVLNALFTHRVGM
                     RLARTRKGDMWKLSTNAAQLFSRDISRNIIELTDTREIETFVEAGTIRREFPGMAGGV
                     VVRSQGTALGCGVMHQGALKSQVPKSRTVISVDF"
BASE COUNT         3939 a         3377 c         2963 g         4157 t
ORIGIN      
        1 aataggaaaa ctgtgcgtcg aaccgaggga taatacgatg gcatcatcgg tggcagccgc
       61 ctgcaattca aaattaaatt tacggcaaaa ttttttgcgc aacgccaatc cccacgcacg
      121 attcatccga ttgccaaacg gagcgtgaat gaccaaatgc atgtcgcccg cttcatcaaa
      181 aaaacgttcg aggatgatcg tatcctgcgt gggcatcgct tccaaagcca aataccccga
      241 agcgacgtaa tgcaccagtt gttccgccgc gcctaatccc aacccgagtt ccgaacaaag
      301 ccattgcacg gccggttgca tccacggtgt ttttcccggt acgaccggac cgtcgggatt
      361 ttgccgaacc gacgcactga gcacctgcgg cttaagcatt tcgaccgaac ccaactgttc
      421 accgattttt tcgcgtaagc gtgcgaccgc ttgggataat tcgattgtgc gccccggcgc
      481 ttcgccgagc caaaacggaa tcgaaggcgg cgcctgtccg gcatccacga cgcgcacgac
      541 accgtcattg acgcgggtga ttttccagga atgattggct aactggaaaa tatcgccggc
      601 tgaactttcg atggcaaagt cttcgttgag cgtaccgata aacgtatccg tcggttcgag
      661 acggacgtcg tactcaaaat tatccgggat ggctccaccg gatgtcaaag cgacgagccg
      721 tgcggcagga cgtgcacgca cgacacgatg gaccatatca tggtggagat aagcaccccg
      781 gcgaccgcgg cgcgtcgtaa atccttccga aagcatggtg agtatttcgt caaacgtttc
      841 gcgcttcagg ttacgaaagg gataagcctt ttgactcaga gcgaataact catcttcggt
      901 gcattcgcgg caggatgttt cggcgatgat ttgctgagcc aaaatgtcaa tcggttgttc
      961 gggcatcaga atccggtcca gttctcctcg gtgcacggcg tccagcaaag ccgtacattc
     1021 gacaagttcg tcccgcgtga gcggaaacaa gcgtcctttg ggcgtgccat gaatgctgtg
     1081 acccgaacgg cctacacgct gcagaaacgc cgcaatcgaa cgcggtgaac tgatctggca
     1141 tacgagatca acggagccaa tgtcaatacc cagctccagc gaagccgtag cgatcaaagc
     1201 gcggagcgat cccgatttga gtcgctgctc cgcgtcaagg cgatgttctt tggacatact
     1261 gccgtgatgc gcgcgtacga catccgcacc gagacgttcg atgagttgaa acgaaagccg
     1321 ctctgccaat cggcgcgtat tgacaaaaat aagcgtcgtg cggtgttcgg aaatcagttc
     1381 gaccaagcgc tcgtagattt cgtcccacac ttcgccttcc atcacggcgc cgagcggcga
     1441 acgcggtatt tcgataccga ggtccatcgc acgtcgatga ccaacgtcaa tgatcgtaca
     1501 cggcgcagca ccgggctcca agcggccggt gagaaaatcg gcaacttggg taagtggttt
     1561 ttgcgtcgcg gagatgccta tccgggtcaa cggtcgggaa accagcgatt gcaggcgttc
     1621 gagcgacaag gcgagatgcg atccgcgttt actgccgacc aaggcgtgga tttcatccac
     1681 gatagcgata cgcgcggatt ggagcatctt gcgaccaccg tcgctggtga gcaaaacgta
     1741 taacgactcc ggcgtagtga cgagaatatg cggcggcgac ttgagcatag cggtacgctc
     1801 ccccgccggt gtatccccag tacgcacttg cacgcgtatc tctacgcggg gcaatcgcag
     1861 tgattccaat tcatcctgta tagcctgcaa agggcgttga agattttttt cgatatcgtt
     1921 ggaaagcgct ttgagcggag atacgtacac aatgcgcgtt tcttgcggaa gcccgcgttc
     1981 aagaccttcg atgactaagt catttacaat tgcaagaaac ccggccaagg ttttaccgga
     2041 acccgtgggt gcggcgatca gcgtatgctt tttttctaca atggcctccc atgcctgtga
     2101 ttgaacttcg gtcggttgcc tgtattcacg gacaaaccag cgtgcaatag ccggatgaaa
     2161 attttgcggg atcgaagcgt ccataaaaaa tacaatgcca tttctacatg gaggtttgca
     2221 aacggtcgtt aacggaaaac atgttaagcg cgctttcgcg cataaacatt agccgatttc
     2281 ctgcggttac cgcaggtttt catactgcac caccgccgcg ttttgttttt tccttggtcg
     2341 taaaaaaaca aacggcatcg gggattcgca cactgtttta tgcgcgacgg atcgccggac
     2401 tccaagagat caaccaacga aacggctagg ggtactacaa aatgctccgg cgattggaat
     2461 gcgtacacgg cttgtttttt aaaacccgac ggcgattcct caagccgtaa caccatgcct
     2521 ccggatgaca aaataaaatt tatctcatcc gtggccgttt tcggtacagg tttatgtgcc
     2581 atgatacagc ttaacacttc aaaacatacc cgccgaaatt tatgaacttt tcgcaacacg
     2641 gcttcggatt cgggtgaatg gatccatatt cgattcagtt cattacgacg cgcatgcgtg
     2701 agtatacccg cctcctccat ccataccaaa atatctttgt tatgctgcaa tgtttctaca
     2761 gtgctgtttt tctgaagata caccgtattg agaaaatcca ttacggtatg gccgcctaaa
     2821 aaaataaaag atggagatgc gtttttcatt tgtgatcttt gaaaatatgt gatcagatag
     2881 ccaattttcg cagtttgata cgagtgtatg tcaaagttta cgttaagaaa aaactatgga
     2941 cgaaaacaat cgagtaccac caattaatcg tagcatgaaa ccgaacctaa tgtaatcggt
     3001 tatcaactat gcattttaaa catagttgtt cattgatcag tttaagcagt gcgttcctat
     3061 tgtacaaggc attttactac gcttcgctcg tgatattaca gtctaatatg aaatactatt
     3121 tggggaaata ttaatcgacg tgatctttat cgtgttttac gataatttag aataagcttg
     3181 aggttggggc gcatacttac agtcctgtaa aaaagaaaat tgttttagtt aattaatcct
     3241 tcatttttaa acaacaaata ctctataggc accaaagaga aaatagaatt aacatcgtgt
     3301 gtatatatct aaaatgagat ttatagaaag caatctgaat caaaccttac aagacagccg
     3361 caatggcgat tttattttct tcactttctc attcgcattc acaagaatcc ctcaaaactt
     3421 tcactacaaa gtttgtcttc accgaattat atgcaccgaa ataaccggct atcttatcgc
     3481 cgtcctcatc cgtgatattc gaacgatacg taataggtgc ataataacgc caatacctca
     3541 tagaatccca aaccgccggc tcaatacttg tcgtttctcg cataccccat gcccgcgtaa
     3601 gattggtgtc cagcgccatg gatgtccagg gaatactgaa gtatgaaatt cctccgcact
     3661 tactaattat gataggagcg gctgcacggt ttgatgacca tagttgcact atatcctctt
     3721 cgttaccata aagatatggc atacgaaagc gatagacata accatccgta actacactac
     3781 cactgacaaa cgtttcccca taacatatgc ttgattgagg agtttctttt ttgggataag
     3841 atagaactgt gtcacctacg gatatattat tgatcgtaat atcaccgggt acttttacaa
     3901 ttccctgata cgttttccct cccgatcttt taacaagcag tgcacaagtc tgttgaccgt
     3961 aaacgtgaag cgcgtccgcc acatcccggt aggtgccttt gccgacatgc tccaaccgga
     4021 cagtctgatc gcctgccgtg atatagacct ccgcatcgtc atggtaggta agcagagctt
     4081 gatactcatc cacaaacgcg cgcagttgta ttcgcatcat atgatcctgc aaattggcag
     4141 cgtccgtcga gtccggaaaa atagtgtgga gtcgtccgac gaaaacctct atcggaaatt
     4201 cacgatcttc atccggcgct acatgcgcca cgaccaattc atagtcctta tcaaggcgcc
     4261 cttcgtaggg ttttgcctta tgatccgtac aaccgaagct acttaaaagc aatacaatag
     4321 aggctactag cttcgacatg gatgccttat ctagcttaat cgcatgtaca agaatcattc
     4381 attgccttta cgacaaagtt aatacttact gaattatatg acccaaaata tcctgcaatt
     4441 ttttttcctt tttgatcggt aatattcgac cgatctgtta taggagcgtc atacttccag
     4501 taactcaccg tatcaatcat atcatcacgt gcgccggttg tttcaccgag gccccaggca
     4561 tgggatagca ctgtatcaaa agccgtagaa gtccaccaaa tgttatttgc atatgatgct
     4621 tcttcgcaat tattaagtgt atgtggacag ccaaaggaat taataaattc caagaagact
     4681 atgcgttcgt caaagttgtg actattatca acttgttgcg aacgataaaa taaagctcca
     4741 tccacttgag gtccaatgat tcttggtgaa ccaaaacaaa gttgagatga ttttcttctt
     4801 tttaaatatg atactattgt atctccgcct tgcacactgc tgagtgatat actacctgga
     4861 actttagcga atgcctggta agttttgccc ttaggccgaa taactttgag agaacaagtt
     4921 tgaaggttag agatatggag tgcattggcg atgtcccgat atgtacctct acctacatat
     4981 tgaagctcca cggtttgatc gcctgccgtg ataaaaacct gagcatcatc atgataggat
     5041 aaggttgcca agtattcgtt ttgaaactgc cgaagctgaa tcttgagcag tggttcctga
     5101 atactggcgt tattcaaagt atcagggcga ggcactagta ggcgacccac aaaaacttcg
     5161 atcggatagg ttcgatcttc gtcgggcata acatggccga tcacaaactc ataatctttg
     5221 tcaagttttc ggcgtaatga tggtttttcg tcgtgttcgt cgaggcagcc aatgcaccct
     5281 gagaaaacaa caacgctctg aataaaaact aaaaaaagat ttttcatttt gttgtccaaa
     5341 attagtgggc tacccgaata ttacttccag atagcccata cttagaatga tctttcaatg
     5401 aatcttaata taccaattga atcatccacc aatccatatc ggtaagacct acaccgacgg
     5461 tattaaatga actttcacca tcgttagttc tttgcgctaa gttagctgca gtgatatttc
     5521 taatcccccc gtgctgatag cggtcatcat tgaccgaatt atacacatct actttgtacc
     5581 cttgattttg tgtgaccatc gatgtcggat cagccatatc ataaaaatct gtttgtgtgt
     5641 agaccacaac attggcgcct tcgtttctat tatatttccc ttgttcgtat gtatggttgg
     5701 attgatctgc ttcgattgcc accatgcgaa gtggtgttga aaggttatca actctaacaa
     5761 gagctatctc ttcattcgga tccacttcgt atgcttcttt gtcattaatg tccagataat
     5821 acctatatac ttcagccgca tctaaatgat tgcctccatc aaaaatatgc gtcgtagtgc
     5881 tagctattgt cgttgaattg ggatcattat atgaaaagta cacttcaaac tcttcatcac
     5941 ttgcatcctc tttgtttttt aagtcaattt tcttgaaggc catatatgtc ccagcaatag
     6001 gcgtggaggc tgattttgcc agattgattt gatcatcatt actttcttta ataaagaaaa
     6061 ttgtttcatt tgttggattc ttaggatcta tggtgatagt agtttccgtt atcgagtttc
     6121 catcataata aaaggctggt tcttctatta caggcaaccc tgtttcttcc tcctgtacat
     6181 cttcgggaaa atgatagaca tcgggatcaa gcccaacata atacgttgtt gaagtagctc
     6241 ccttagccat cacattcgct tgagacttgg aatacaactc aatctcaact ggaaagttat
     6301 aatgacttgt ttttgaaaga acggaactgg aaggcaactc attgagtcga atactacttt
     6361 tacttaattt tgtaatagtt tcttgcgtaa tccatgaagg atcggcagct aaaatgctag
     6421 ctatttcatt gagcgcttca cgcgtaagcg aaacatcgtt gtcatattga gcgcctttat
     6481 cttcattcgc tttttcctga cacgccatcg tccataccat aaaaaggcag atcgggacga
     6541 ttttccattt tgctgttttc atttgtaaga ctccctttag aaaaatttgg ggaaacaaat
     6601 gattgctaat ccatttgtga tatattagat tggcaacggg aagatcatat tactttatga
     6661 ttcaatgccc caagcgctga atttggtaat atgcatcttc cttttttgtt tcggttgtga
     6721 aaaaattaat tggaccgact gctatctttg taaattgcga atgccctcac ctcctttttt
     6781 ttggaatttc aaagtcatac caacttcaat tcaactcaat catcgagctt agannnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntggctta gatggcttag atggcttaga
     6961 tggcttagat ggcttagatg gcttagatgg cttagatatt ccctataata cccataatat
     7021 atcctacgat aagaatataa caaaaaaagc agctgaaatc tgtaggtatt ttactgaaaa
     7081 tcttgtaggt aatttaccta caagatttcg ctaagagtaa aaaaatcaac aaaaacagat
     7141 agcctttcaa aaatatttca taggttacaa aaacaatctc tcctgctgat ttgcctaaag
     7201 agtcagtggc cgcctaaaaa aataaaagat ggggatgatg tagtcatata aaatcttcgg
     7261 cgaggttcta aacctctata tgtatcaatt ttttacaccc tgcattctta caggaaattc
     7321 gttagattga ctttccataa ggcattacgg ttagctcaac tcgtgccttt aaaataatac
     7381 atacaatttc aaaataatta tttaatttta gttaaccgac cttacgtata tcaagagatc
     7441 tttacatgga aacatcatcc ttatttacca tcatcgccgc atttctctcg caatcaccca
     7501 ttgtcgtcgt atggattgtc ggcgcggtat tggcttatcg gcaaaagcct gtttatccgc
     7561 gaaaatcgct gttgatcatt attgccatgt tgctttttct cggccaactt tttattaaca
     7621 ccattcttaa tatcgtattc ccgatgatgt gggataccca gcaactgctc ctgagctaca
     7681 acgtcaaagc gatggttaca tccatgatca tgacggcggg ctggattttg attatgatgg
     7741 cgttatttat gaaggaaaaa tcccgtgacg gattcggaca aaactgagaa aatttaccgg
     7801 atcgcgagaa aaggttccaa ggtttgtaag cgctcgtcgt ttttataata tacccgttct
     7861 aaaaacaatc ccgacggcgg cgccgtgaga cgcgccagtg acggcgacgt ttgacgaaaa
     7921 aggcctgcga tatcgcccgg cttcatatta ccgcgcccga cttccgccag tattcccgtt
     7981 atctgacgca ccatcttcca caaaaaatga gaaccgacga cccgtatcaa aataagatca
     8041 ccccgcgact cgatgcgcag ttcttccagg ttcactttgg ttgaatcggc ctccccgtct
     8101 ttggccgcaa agtttttgaa atctttgagc ccgacaaaaa aacgggacgc ctcttgcatc
     8161 cgggcaagat caagttcgtc tttgatccac cacacaaaac gttttccgaa cgcactgcga
     8221 cgacgggaaa tctgatagag atagctccgc gcgacggcat catgacgcgc gtgaaatgac
     8281 cccggcgctt tttcgatttg tttgatgtga atatcgtgcg gcagacgatc attgaccgcg
     8341 aggcgaaatt tatacggatc cagaacggtc gtacaatcca ggtgggccac ttggtgcagt
     8401 gcatgcacgc cgctgtccgt tcgtcccgcg ccctgaaaat caaaattttt gtttcgcagc
     8461 tcttccgtga tcgcgtacat cagcgtgccc tggacagtat gtgtatcttt ctgcacttgc
     8521 cagccgctat accgcgtgcc ttcgtattcg agcgtgagtt tgtatcgggg catgatttaa
     8581 acaagaaatt gcaaatcaat atctattgga tcgtgattcg cataaggaag gaattcggga
     8641 cacgaaattg tcattaacca ctcaacttga aaaataataa agatttaaac ttgctcgtga
     8701 tatgctgagt ttgtcgaagt atcaccacgc tagacgcaat atccgagttg tatgaaacct
     8761 cggtttctat atctctataa ttttttgttt aaaaaaagaa cttcagaagg tttccaaccg
     8821 gtcgcatttc ggtcagcgag ttcaagccaa cgttttattc ttttgttagg cggaacagat
     8881 tctttccaat actctacaaa taaagcgtct tccattgttt ttgcggcatt tatcatttca
     8941 tcttcaatgt ctgataacgc cctgtgaata tacaatccac ctcgaatact gtcagaagcc
     9001 attgcaacac ttcctgctga aaataaatct cttgcttggg aagccaaaac accgtgataa
     9061 gttaataaat acatagcaac atcattgtat ttatttttca cacgcgaacc cgcaacaatt
     9121 ttcggacaca ataaagatgc ttctgctctc agcttccttt tttctgattg cgaaagctcc
     9181 gtgtatagtc taacctgaat aggcaaagtg tgttcttgtt cgggtttttc ttctatccac
     9241 caaagacgtt cgccttcttt taatcttgcc tttgcgtatt ctctgatata acgcattttc
     9301 ttttcaattg gtaaaacaga aaagtcatcg tatgaaattt tcatttgctc aaataatgaa
     9361 gatttcgcgt ctatttctaa ttcaaacctc ggaacatgcg atgtcctaac gtgaattacg
     9421 cactcctgat atgattgcca ttttacctcc ggcaaaccac ccattttacc aaacacaaca
     9481 taaataaata caacctcttt gtttcggaat gtttcaaata cactgttagc aacacttctc
     9541 caattatctt tttctgtgaa ttttacttct attccgtact ctccaacggc aatatcggga
     9601 aatgcataag ggtgcggatt gtaatctatt tcaactttac tatctgtgat aagagtttta
     9661 agaatgtctc ttaccctatt ctcaaatact tttgacgaag aaaatacatt tttttgcgct
     9721 tcgtacgtta attgttgcgc caatttgtta agaatatctt caaattcttt ttttttcatc
     9781 ttacaaaaga cagtctttta ctgattgcgc aatgtgccag gccaatacag gcgggacagc
     9841 gtttccgatt tgtcgttcag tttctctaag ttttgcttca aagataaaat tgtccggaaa
     9901 agactgaata cgcgcggcct ctctcataga taatctacgt ggcaatgaat agtgaaattg
     9961 aatattcccg tgacattcag ccctaattgt gaaagcgggc tttgatggct ctaatcttct
    10021 tccgccttgc tcgccgcttt tttttgctaa actccatata tggttaatct gctcattttc
    10081 atttaccttt tccaaatcgt gaattgcgtc ataacttgta agccaatttt ctttttgtct
    10141 aactgtttta ggcgggacaa acgacttaac gtcttttgcc gttccaacaa taaaaacacg
    10201 ctctctactt tgtggaacgc cataatccgc tgaatgatac aactgataac ttatattata
    10261 tcctaactca ttaaaatcgt gaagaatctt attaagtgat tctcgtcgcg tcttcattaa
    10321 taatcctttt acgttttccg caacaaatac ttttggattc gtgagtttta ttgcttctat
    10381 catataagta taaaggctac ttctttttcc atttacaccc aaaccttttc cgtttattga
    10441 aatgtcttgg caaggaaaac ccccaattaa aacatctgct tgggaaggaa gttttcctat
    10501 ataatcacga atatctccgc aatgaatatt actgtcaata tttcttgaat aacttttaca
    10561 agcatacgga tttagttcat tagcccattt aatttcaaat ggatttttat aatattcatt
    10621 tccgaaaatt gaaaatccgc ctataaaacc caaatccatt cctccgcaac cagaaaacaa
    10681 agaaactatt gaaaattttt ttttcttttt caaaatactt tgcgaaaccc ttctttgcat
    10741 tgttttcaac ttaacgattg tcgtttcgtt agcggcaatc gttagaacgt cattgtccaa
    10801 ctctgcatat tgttctctta ttgctgtttt ctgcatatca attatgcctt tgctttttgt
    10861 aatacttcac tttttcttcc gcaattttga gaatgtcttt tgtataatct tcttccatta
    10921 tttcataagc gactttatcg cttaggaagg aaacaagtaa tggctttgaa ttaacggaaa
    10981 acagttcggc taatttttca attatttcct tgttcgcgtt gcgttcattc ctttcaatct
    11041 ttccaagagt tgacggatca atatcgagat gcgccgctac ttttcgaaga ggcaaaccgc
    11101 tttcttccct tagttttctg attgtgtctc caaaagtttc attttgcatc ataatcatga
    11161 atagtatata gaaaattgat tcaggacaaa cagtgtccaa acatacgatg cccttaaaaa
    11221 ggaatcaaca attttgtctt tttataaaat atttttattt aaaacctaaa attcggagag
    11281 tgaatgagct tggttgcctc gcttttctgt ctgtcaaatg taactttgat gatgactcat
    11341 tctatgggcg cccgtatctc aatagtcgaa cacattaaaa caagaaaacg aaaaaaggaa
    11401 agaaaaaatc caccaacaat cctgctaatt atggacaatc ctattatcga acagcagctt
    11461 gtttaaacat tttaaatcaa cccaatttgt agattaaaaa actccgagcc atgaccggct
    11521 cggagttgta atattaattc aaaaaatcag atccgcgatc acatgccggt aatattatat
    11581 ccgcagtcaa cgtacatgat ttcaccggtg ataccgcgcg aaagcgggct gaccataaac
    11641 agcgaagcgt cgccgacttc gtccacttcc acattgcggc ggagcggcgc tttttgaggc
    11701 gtgatatcca gtattttggt aaatccggaa atgccgcgtg ccgctagcgt attgatcggt
    11761 cctgccgaaa tcgcattaac gcggatattg gacggaccga gatcggctgc cagatatcgc
    11821 accgaggctt cgagcgatgc tttcgctacg cccatcacgt tgtaaccggg tattactttt
    11881 tctgcgccat agtaggtcaa ggctacgatc gcaccgcctt tttctttcat cagcggcatc
    11941 gcttcgcgtg ccataccgat caatgaatac gcgctgatgt cgtgggctac gcgaaatcct
    12001 tcgcgcgaag tcgtactaaa cggtttctct aaatcgtctt tctgtgcgta agcaatggag
    12061 tgcactaaaa aatctatatg cggcatgtct ttgctgacgg cttcaaacaa ccgcttgata
    12121 tcgtcatctt tggtcacatc gcaatcataa agcagattac ttccgagttc ggttgcgatt
    12181 tcttcgacat tgcgcttgag gcgttcaccc tggtacgtaa aagccagctt ggcgccttcc
    12241 cgtgcgaggc ttttggcaat ggcatgggcg atactgcgct tattggcaac gccgcaaatc
    12301 aatccttgtt taccctgcat cagcataatg atagtactcc tgattgttag tgtaaaaacc
    12361 ggcggaaata tagccaaacg aaaattgcat aacaactaaa agattcccgt caccggtcgg
    12421 acgcctcaaa acggtacggc accgacgtat ttcaaaaatc cacactgatc acggttcggc
    12481 ttttgggcac ctgacttttg agagcgccct gatgcatcac gccgcatccg agcgccgttc
    12541 cttgcgaccg gactaccacg ccgccggcca taccgggaaa ttcacggcgt atcgtccctg
    12601 cttcgacaaa cgtttcaatt tccctcgtat cggtcaattc gataatatta cgggaaatgt
    12661 cgcggctaaa aagttgcgcc gcgttggtcg aaagtttcca catatcgcct ttacgggtgc
    12721 gtgccagacg catccccaca cgatgggtaa aaagcgcatt gagcaccgat tcgcgaccgc
    12781 gccaatcttc cgatgcgatg aatgtttcat cgtctttgat gtgataaaca aattgatccc
    12841 aaaagttttc atcaaaaccg aaaaggccgg aaaaatattt tcccatgccg cggatttccg
    12901 catgcgagga tccatccagc gcatacattt ttttgtgttc cgcttgcgcc gttttaccgg
    12961 tgcgcacttc gatggcctca tccagtctga atttggcaag gaaaaatcct tcgggattga
    13021 tcgctgacgg catcacgcga cgggtcagcg ctacgcgcgg gtcaaatatc ttttccccca
    13081 gcgccgtaat gcccggcagt gcggctatac cctcgggcag atcaaacgct tctaaatgca
    13141 aagacggata ccgtttgagt atcgtatcca ccaatccttc attttcttcc gccgaaatcg
    13201 tgcaggtcga atataccagc gtcccgccgg agcgaagggc tttgagtccg ctcacgacta
    13261 attgttcttg tataaacgca aatttattca gatgacgcaa attgctgacg gcggatggcg
    13321 ctttatgaat tacccccagc gccgaacacg gcgcatccac caatactttt gtaaacgatc
    13381 cgggtaccag atttccgatg cgctcgccct gcatattgac catggcggta ttgatcacgc
    13441 cgaggcgatc cagattatgt gaaagggttt tgatgcgttt gtcgtcgcga tcattggcga
    13501 ccatacagcc gccatttttc atcatctgcg ccatttgtgt ggttttggaa ccgggtgcag
    13561 ccgacacgtc caaaacaaaa tccgacgccg tgggatttaa cactatcggt ggcaacatcg
    13621 aagcgatact ttgtatataa aataagccga ggaagtgttc aatcgtttta ctgatccgcg
    13681 catcttcgcc ttccaccaga agtgcatccg gcatggacgg gatttccgta aaccgaaaat
    13741 tttgttgcgc aagccgatgg atgaaatccg ttttgttgaa cttcagcgta ttcacacgaa
    13801 gcgccgtacg ccgctcaaaa tccaggcctt gcgaaaacgc gtccgcgtat tcgccgggaa
    13861 ataatttaag atattctttt aacgcttccg gtatttcgat cggcataagt aaagtgtgtt
    13921 aaactgtatg ttgcaatcgg gctaaataac aaccttttgg caatagtgca acgacgaatt
    13981 gtaaaatcgc gtcattataa cgaataactc ttcttcattt ccctttgcga ctttgcgaga
    14041 atgaatgcgg cttcactacg gcgcgtttgg ttcgatcatg cctatggcgt gaatcaccat
    14101 gaaatacctt gacttggaaa ttatgaaacg tattgtaaca caataaacaa gtagaacgac
    14161 gactgcgttg tctcacctgc gcaagcgggt gtctatacaa tttgtggccc gcaagaacaa
    14221 atacatgctc gctaagccgc tgagacgcca aggcataaaa tcgtaaaaac cttttccttt
    14281 tccctttgcg actttgcgag aatgaatgcg gatttgctga ggctaaaaaa acggcatcaa
    14341 cgatctcata atttactcaa actttaataa ccacgaggaa ttcgagaaca cttttgaagt
    14401 gaccgggcca ttacgagata tttacataac ataatctttg cctttgatcg tacggcatcc
    14461 ttagattgcg cgcaatctac aaaggatacg catgtttgct gaagttgttt ttgacctcgc
    14521 gatcgaaaca cgtttt
//
LOCUS       DAOQNQ010000051        14323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1505_length_14323_cov_5.811186, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000051 DAOQNQ010000000
VERSION     DAOQNQ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14323
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1505_length_14323_cov_5.811186"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PK754_05145"
     CDS             <1..363
                     /locus_tag="PK754_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNB08921.1"
                     /translation="IMLGTYVLSAGYYDAYYKKAQKVRTLIKKDFTQVFSQGIDCILA
                     PTSPTVAFKFGEKSNDPLAMYLSDVYTVNINIAGNPAISVPCGKNSENMPYGVQIIGK
                     DFDESMCFRVGAAIESLR"
     gene            374..2095
                     /locus_tag="PK754_05150"
     CDS             374..2095
                     /locus_tag="PK754_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08922.1"
                     /translation="MLSKFRTFAVFNIVLFTCFISAIGAQTALPKDKIFRQLYFQPGD
                     WVTIGGTRYATCVASTFNMAYIGTLGGIIRYDIIRRQFLYPLTISDGLPENEIFLIGA
                     NSQSQLVWIITRHFICTFNPNTSRVQDVTPIEYLNVRDASTLDAIGFGAGSVWLRSED
                     RYWNSSLSYQSFSPSELPKKETIEWNNIPASSEIASRSIFVSNESGYIFIPHSNLFQD
                     RELRQFPITGYLQDVNYNTWVTTAGAGAWFSNNGSSILSPLNYGLNASSVKAMAFDGA
                     DMWIGATANTLPGQYPSELSGLTQWNQKNDHFTYYFSSLHDNLALNEITDIEADENYI
                     WVGTTSGLNYLNKKSNQWTIAKTYSGLKDDHIIEIVPFQKGIIVATENGLHRSILTKK
                     GIQFQPLEIPDLWNRHVLKILPSGNSIWLGTDNGIYRLDSSFTIQNHYTALGYDIPLN
                     SLVNQVVPAITEMANNLFFISTHSVLRFDKHKKEWESMPISGSYLQSGVYDAIADEDN
                     LWIGLADGALQYDFELQRWAYYNKKDGIGGYSVYKILPDGDYVWFATENGLTQFYWNA
                     EHLRSQK"
     gene            complement(2096..3271)
                     /locus_tag="PK754_05155"
     CDS             complement(2096..3271)
                     /locus_tag="PK754_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB08923.1"
                     /translation="MTPYIFIALSIWSLTISGCTSKENNKSHSDHADLYYCPMHKEVT
                     SDKPGVCPICQMDLVKKSRVESAADTMHHGLFLNPDQMRSAQVTTMKVRTSKLNKTIT
                     GFTTLEFAEDLRKTIAARFSGRIEKLYVAKTGDRIEAGQALFEIYSPELVQAQNDFVV
                     LLNNKEGQSYTNQMLSAAETKLRLLGVTSDQLETLRRTREIKQNFIYHSPYSGTVIEK
                     KITEGAYVNEGTILYDMADLSLIWGVSDIFESEARFLKLHSTVTIRLDTYPDEKFTGT
                     ITLIYPVIEPVSRTIKVRIEINNKDYRLRPKMYGQMIFDSPLPELPVIPETAVLLTGK
                     RALTWVMTSPHHFEQREIMLGEKVGDQYTVLSGITAGEVIVKTGGYLIDSESQLTGK"
     gene            complement(3288..6599)
                     /locus_tag="PK754_05160"
     CDS             complement(3288..6599)
                     /locus_tag="PK754_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNB08924.1"
                     /translation="MIENIIDWSARHRWMVLTAYVAISLYGIYSFREMSVDAIPDLSE
                     NQVIVYTEWMGRSPQVVEEQLTFPLVTALQGLAHVKAVRASSMFGMSFVFVIFDDNTD
                     IYFARSRVQERLSTVRGSLPKDAIPTLGPDGTGIGHIYWYTVEGGEQDLGTLRSIQDW
                     YIRYQLSSVEGVAEVASIGGHVKQYQIDLNPVRMRAFGIDIMDVTQALQRNNNEVGGK
                     IIEWSSAEYFVRGQGYIRTESDIRNLVLRNNGSGIPVLLGQIADVQIGGDIRRGSLEK
                     DGEGEAVGGIVVMRSGENAKAVIDRIKKKINEIAPGLPHGVVIKPSYDRSVLIEQSIE
                     TLNRALFEAALTVSLMVAVFLLHARSILRIIIEIPVSILLSFILMRIFDIHANIMSLG
                     GIILAIGVIVDASIVMVENAYRNIAQALETKGKLLTEDYQAIALQSAKQVGRAIFFSE
                     LIILVSFLPVFMLTGQEGKLFAPLAFTKTFVMLASASVVLTLIPVLMTFLMRGKFRPE
                     KENPVTHFFIKLYEPVMHWVLRYRKITLSLNVIALIITIPMIVNMGSEFMPPLDEGSI
                     LYMPVTLPNASLTEVNRILTIQDRLIKAVPEVAHVLGKAGRAETATDNAPISMIETII
                     LLKDKKDWRPGITKKDIIQELDSKLQIPGVRNGWTQPIVNRINMLATGVRTDVGFKIL
                     GNNADTLEYYAIEAEKILKNVHGASDVVAERGQNGYYLDITIKPEAAQRIGFTNGDLQ
                     EVIEMAIGGQNIGTVLDGRMRFPIRARFGREWRNDTEALKQLWIPIRPRNTMASATPI
                     ENLSYESTKTSMPASMTQITGVVSEPIRQSLLTDVNEGVIFLPLGQLAEVKVNQGPTM
                     VNSENGMLRSVVYMNTAGRDLDHVVEDAKVLIQSELKLPEGYTYQWSGQYEHKLRAQR
                     TLMIIIPIVFILIFLLLYFTVRDPIEALVVMLSVPFALIGGVYMNFILGYNFSVAVWV
                     GFIALYGIATETGVIMVVYLHEALDRRLQSKNGVITQKDIYEATIEGSVLRLRPKLMT
                     VGTALIGLVPIMWSTGTGADVMKPLTAPMIGGLITSAIHVLIVTPILFAIMKERALSK
                     GTLKKSKMAHWIKES"
     gene            complement(6592..7866)
                     /locus_tag="PK754_05165"
     CDS             complement(6592..7866)
                     /locus_tag="PK754_05165"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNB08925.1"
                     /translation="MSGSLKKIALIIGLASLEIPQVHTQVLIDSLIREAIQHHPKIHA
                     IQHNYEAYRFQAKQKKSWPTPDVGFELFQMPASNFPIPVKGNMETDYFIQQMIPWPGK
                     LEAMSEAAHYTAEIQKTMIASAQLNIERDARVAFFELCMINERRRNNANAQRTLEQLL
                     QLSLKRYEVGQITQADILQIENERLALENKAFELEKDRRVTESMLNGVLGNNEIRWPD
                     SIPVDWTNNFSVVLDTLIQNTLPKKPEIQGMNHEIAMFTAEKKAAHREWFPDIMVRGM
                     YKDMSMGKNYWSLMLSANIPFAPWSRGSLDARIKELDMHIKHGAMEIENMKIMITSEA
                     RSAIERIKSSRRQAEQIEQRILPHYEAAYQSMMISFQTGKADTAMLLSMLRMIYMTKD
                     DLVMALAGSKMAEADFYKAIGTSNMKSEAHHD"
     gene            complement(7939..8358)
                     /locus_tag="PK754_05170"
     CDS             complement(7939..8358)
                     /locus_tag="PK754_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08926.1"
                     /translation="MNNQSLQKVLSLLLALVWVTGCLANVRITGTCYSTKTNSMSCCQ
                     SSTTMAGTSNSLQIAAYKSCHCPGMMSVLPERGVSLPIETLKTNSVQQTHALLNFVFQ
                     TAFDQAYNKNSFFDFSHQHPPANTASETNALLSTFRI"
     gene            complement(8362..9510)
                     /locus_tag="PK754_05175"
     CDS             complement(8362..9510)
                     /locus_tag="PK754_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB08927.1"
                     /translation="MTEKKRILVVDDEESIRLICEMALQQNGFKTFSASDGSEALKVV
                     ENQNVDLILSDIFMPMMNGFQLLQSLKKDPYYNQIPVIFLTAATDNDHIIEGLQLGAE
                     DYISKPIRIKEVIARVQMVLSKIEARHQTKPQPQQGHAVKADLDTRRENKPVGKLEEK
                     PLIDVIAFCEANSLTGELIVTHENRRGKLMYRKGELLEVSLDDKKDDAAIDELMSWQN
                     GTFEINQQLLTLDALTKSLRSDAKSTMSSKEGGMAPAPLGSKAVSIPADAVIAALNAA
                     TMHVGQSMGEAKVGNYFRRAQLAHVNKWNTLGYFVVDQRGKTTALKPIQLNGDDIVGV
                     SEWIKSFTRLCAEKDPQWSDMNVVQIVKNNIHDSHWKLLDSAGFPGYF"
     gene            complement(9507..10442)
                     /locus_tag="PK754_05180"
     CDS             complement(9507..10442)
                     /locus_tag="PK754_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08928.1"
                     /translation="MAFKNLVILTMIAGSIFAQQKNIKEPFGMVISLRANIEAKGDKA
                     IKQGDLLYKGDVIDLGQGDYCKVFWADGAVKEYNGKFNVPDLKQLRKEILAMFAEQSA
                     TQSWFFKAQSHYSKQIYRSDAEVSLSAITPRNTRLIDIPAQLIWRHIPDKITGASEFV
                     VHLRSYENDYSFEETVLGNSFTFRNEMNMERGIKYFWFTEAVNENINEATESVWFYVM
                     NKEERTEFENELRESNDAIELTEDTVLKKLLTATIYMRYQLYKDALEITASVLKEQPH
                     HIMALKIQARIYEIIEQPVETKRLLEITQRYMENQ"
     gene            complement(10449..11048)
                     /locus_tag="PK754_05185"
     CDS             complement(10449..11048)
                     /locus_tag="PK754_05185"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S single cluster domain-containing protein"
                     /protein_id="HNB08929.1"
                     /translation="MISVAKILPISEVNGPGKRCVVWVQGCPKRCPGCWNPEFLSFEK
                     STNTVSAKELVTMISKATHGFRDIEGVTFSGGEPFAYAECLTEVAQLLRKQNLTIMSY
                     SGFTVEEIRTKGNFYVSLLEALDILVDGEYKRELNVNRLWRSSANQRVLFLTDRYIAY
                     QAHIDEEYRELEFTLNTNQITTTGFPEVSILKNLTNHRE"
     gene            complement(11107..11604)
                     /gene="nusB"
                     /locus_tag="PK754_05190"
     CDS             complement(11107..11604)
                     /gene="nusB"
                     /locus_tag="PK754_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNB08930.1"
                     /translation="MNPLLADDGAKPGKNTAPKNSRREARETVLKALYAYEMTQDNIK
                     RVLDDVCDNFTEPATQFIHTLVQQTIAHQMEMDVLIRARAQNWEFERIALIDRILMRM
                     ALCEILFFEDIPPKVSINEMIEISKRYSTDKSSKFINGILDSIYEDLKNVGRITKTGR
                     GIIES"
     gene            complement(11643..12746)
                     /locus_tag="PK754_05195"
     CDS             complement(11643..12746)
                     /locus_tag="PK754_05195"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNB08931.1"
                     /translation="MRTFEMIEEKEHEQVVFFSDKASGLRAIVAIHDTTLGPALGGCR
                     MYPYATTEEALNDVLRLSRGMSYKAAVAGLNLGGGKAVIIGDPKKHKNEILFRTFGRF
                     IQGLGGRYITAEDVGTSVTDMEWIRMETRYVTGISRAQGGSGDPSPVTALGTYRGLKA
                     CVEKVFGTQSLKGLRIAVQGLGHVGYYLVRHLHDEGAKLVVTDVDGERVKRVVSEFGA
                     EYVDSDKIYDADVDVFSPCAMGAIINDDTIGRLKCKIIAGAANNQLQDEARHGAMLLA
                     KKITYAPDYVINAGGLINVYNELEGYNQEKALAQAKGICDIMKKILELAERENISTHE
                     ASNRIAEERIRQIGHIKSHYVTQPESVLKRIER"
     gene            complement(12777..13916)
                     /locus_tag="PK754_05200"
     CDS             complement(12777..13916)
                     /locus_tag="PK754_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003008290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNB08932.1"
                     /translation="MDFKLSEEQEMIRQTARDFAQEHLAPHAAERDEKQFFPKDVIKQ
                     MGELGFMGMMVNDKWGGAGLDTISYVHVIMELAKADASAAVIASVNNSLVCFNIEKYG
                     TDAQKEKYLKPLATGGALGAYSLSEAVSGSDAAGLICFAENKGDHYIVNGTKLWVTNG
                     YHSDYVVCFIRTDKSNKTKGISAFVVDKSFPGFKVGKKENKLGIRASDTTELVFENCK
                     VPKENLLGQEGLGFKIAMETLDGGRIGIAAQAIGIAEAALEETVKYTKAREQFGQPIA
                     KFQANEFKIADMAMRIDAAKLLLFRACTLKDQGQKFGKEAAMAKLFASETAMWVTTQA
                     VQMHGGYGYTKEYPVERFMRDAKITEIYEGTSEIQRVVIARNVLG"
     gene            complement(14067..>14323)
                     /locus_tag="PK754_05205"
     CDS             complement(14067..>14323)
                     /locus_tag="PK754_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08933.1"
                     /translation="TKHMAHFMQRKACLEIKSGNNRPGVKLAGEAYELGWKTKEQKTN
                     YEIAAQIWNEHQRGSKITTEWVEYWQKHFNYWVADKELAI"
BASE COUNT         3880 a         3309 c         2835 g         4299 t
ORIGIN      
        1 atcatgcttg gtacctatgt gttgagtgca ggttattatg acgcgtatta caaaaaagcg
       61 cagaaagtcc gtacactgat caaaaaggat tttactcaag ttttttcgca aggtatagat
      121 tgcatcttag cgccgacgtc accgacggta gcgttcaagt tcggtgaaaa atccaacgat
      181 cctttggcca tgtatttatc ggatgtttat accgttaata tcaatatcgc cggtaaccct
      241 gctatcagcg taccgtgcgg caaaaattcg gaaaatatgc cttatggcgt acagatcatc
      301 ggtaaagact ttgatgaaag catgtgtttc cgcgtgggtg ctgcgatcga atcattacgc
      361 tagtcggagg ccgatgttgt cgaaatttcg tacatttgct gtgttcaata tcgttttgtt
      421 tacttgtttt atttctgcta ttggcgctca aactgctcta cccaaagata aaatcttcag
      481 gcaattatat tttcagcccg gagattgggt aaccatcggc ggtacgcgat atgctacttg
      541 tgtagcttcc acttttaaca tggcttacat cggtacactg ggtggtataa tccgatatga
      601 catcatccga cgtcagtttc tctacccgct gaccatcagt gatgggctac ccgaaaatga
      661 aatctttttg atcggtgcaa attctcagtc acaattggtc tggatcataa cgcgtcattt
      721 catttgcact ttcaatccca atacatcgcg cgtacaagat gtaacaccca tcgaatatct
      781 gaatgttcgc gatgcgtcaa ctcttgacgc gataggattc ggtgcaggtt ccgtttggct
      841 gcgttcggaa gatcggtatt ggaactcctc tctttcgtac caatcatttt caccatcaga
      901 acttccaaaa aaagaaacta tagaatggaa taacatcccg gcgtcatcgg aaatcgcttc
      961 acgctccatt tttgtcagta atgaatcagg atatatattc ataccccata gcaacctttt
     1021 tcaagatcga gagctgcgcc agtttcccat caccggttat ctgcaagatg tgaattataa
     1081 tacttgggtc acgacagcgg gagccggagc ttggttttca aataatggtt catccatctt
     1141 atcgccactc aattatggac tcaatgcttc gtcggtcaaa gcgatggcct ttgatggtgc
     1201 tgatatgtgg atcggtgcga cggccaatac tctaccggga caatatccga gcgaactatc
     1261 cggacttacc caatggaatc agaaaaacga tcacttcacg tattatttca gcagcttaca
     1321 tgataacctt gctctgaatg aaatcacgga tattgaagcc gatgaaaatt atatttgggt
     1381 tggtacgaca agtggtttga attatttgaa taaaaaaagc aatcaatgga ctatcgctaa
     1441 gacatacagc ggtcttaagg acgatcatat catagaaatt gttccgtttc aaaaaggtat
     1501 tatagtagcg actgaaaacg ggctgcatcg cagcatactc accaaaaaag gaattcaatt
     1561 ccagccgctc gaaattcctg acttatggaa ccggcatgtt ttaaaaatat tgccctccgg
     1621 aaacagcatc tggcttggca cggataacgg tatttatcgt ttagattcca gttttactat
     1681 tcaaaaccac tatacggccc ttggttatga cataccgctc aattcattgg tcaaccaagt
     1741 cgtaccggct ataaccgaaa tggcgaacaa cttatttttc atatcaaccc atagtgtatt
     1801 acggtttgac aaacataaga aggaatggga atccatgccg atcagcggtt cgtatcttca
     1861 atccggcgta tatgacgcta tcgccgatga agataatctt tggatcggtt tagcggatgg
     1921 cgctctgcaa tatgattttg aacttcagcg ctgggcttat tacaataaaa aagacggcat
     1981 cggcggctat tcggtgtata aaattttacc cgacggtgac tatgtttggt ttgccaccga
     2041 aaacggattg acacagtttt actggaatgc cgagcattta cgaagtcaaa agtagttatt
     2101 tgcccgtaag ttgactttcc gaatctatca aatatcctcc cgtttttacg atcacttctc
     2161 ctgccgtaat gcctgaaagt acagtatatt gatcgccaac tttttcacca agcattattt
     2221 cgcgctgctc aaagtgatgg ggcgatgtca tcacccatgt caaagcacgt ttgccggtaa
     2281 gaagcactgc cgtttcaggt ataaccggta attccggtaa gggggaatca aatatcattt
     2341 gtccgtacat ttttggccgc aaacgatagt ctttattatt gatttcgata cgtactttga
     2401 ttgtgcggga tacgggctct atcacggggt aaataagtgt gatggtaccg gtaaattttt
     2461 catcgggata tgtatccaac cgaattgtta cagtgctatg caattttaaa aaacgggctt
     2521 cggattcaaa tatatccgaa actccccaaa tcaaactcag gtcggccata tcatacaaaa
     2581 tcgttccttc attgacatag gcgccttcgg tgattttttt ttcgatcact gtaccactat
     2641 aaggggaatg ataaataaag ttttgtttaa tttcgcgcgt gcgccgaagc gtttccaact
     2701 gatccgaagt aactcccaat aatcgaagct ttgtctcagc cgctgaaagc atctgatttg
     2761 tataggattg tccttcttta ttatttaaca gaacaacaaa atcattttgc gcctgaacga
     2821 gctccgggct atatatttca aacagcgcct gaccggcttc gatacgatcc cctgttttgg
     2881 ctacgtataa cttttcaatg cggccgctaa agcgtgctgc aatggtttta cgtagatctt
     2941 cagcaaattc taacgtcgta aaacctgtaa tcgttttatt tagtttggac gtacggactt
     3001 tcatcgttgt aacttgggcc gaacgcattt gatcaggatt aagaaataaa ccatggtgca
     3061 ttgtatcggc agcggattcc acacgcgatt ttttcactaa atccatttga caaataggac
     3121 aaactcccgg cttgtcggat gtcacttctt tgtgcatagg acaataatac aaatcggcat
     3181 gatcggaatg agacttgtta ttctctttcg atgtgcatcc gctgatcgtg agcgaccata
     3241 tgctgagtgc aatgaatatg tagggcgtca tataattttc tttcgtttta actttctttg
     3301 atccaatgag ccattttgga cttttttagt gttcctttgc ttaaagctct ttctttcata
     3361 atagcgaata aaatcggtgt tacgatcaaa acgtgtatcg ccgatgtgat caaccctcca
     3421 atcattggcg ctgtcagcgg cttcatcaca tccgcaccgg ttcccgttga ccacattatc
     3481 ggcaccaacc caatcaaagc cgtaccgacc gtcatcaatt taggtcgcaa tcgcaataca
     3541 ctgccttcaa tcgttgcttc ataaatatcc ttttgagtta taactccatt tttggattgt
     3601 aaacgacgat ccaaagcctc atggagataa actaccatga taacacccgt ttctgtagca
     3661 ataccatata acgctatgaa accaacccat acggctactg aaaaattata accgagtata
     3721 aaattcatat atacaccgcc gatcaaagca aacgggaccg aaagcatgac aacaagagct
     3781 tctatgggat ctcgcaccgt aaaatagagt aataaaaaaa tcaaaataaa aacgatcggg
     3841 ataataatca tcaatgtacg ctgtgcgcga agcttatgtt cgtactgccc gctccactga
     3901 tacgtataac cttcaggtag ctttagttcg gattgtatca aaacttttgc atcttctact
     3961 acatgatcca aatcgcggcc cgctgtattc atatagacta cggaacgaag cataccgttt
     4021 tcactgttaa ccattgtagg tccctgattg actttgactt cggctaactg acccaacggt
     4081 aaaaaaataa cgccttcatt aacatctgtg agtagtgatt gacgtatcgg ttctgatact
     4141 actccggtaa tttgcgtcat cgaagccggc attgatgttt ttgtgctttc atacgagaga
     4201 ttttcaattg gtgtggctga agccatcgta ttgcgtggcc ttatgggaat ccacaattgt
     4261 tttaatgcct cggtatcatt gcgccactcg cgtccaaatc gagcgcgtat gggaaaacgc
     4321 attcgaccat ccaatactgt gccgatattt tgtccgccga tcgccatttc tataacctct
     4381 tgcaaatctc cgtttgtaaa tccaatacgt tgtgcggctt caggttttat ggtaatatcc
     4441 aggtaatatc cattctgtcc tcgttcggcc actacatcac tcgcgccatg gacattttta
     4501 agaatttttt cagcttcgat ggcataatac tccagcgtat cggcattatt acccaatatt
     4561 ttaaaaccca cgtcggtacg tacacccgta gcgagcatgt tgatacgatt aacaataggt
     4621 tgtgtccatc cgttgcggac gcccggtatc tgtagtttag aatctaattc ctgaatgata
     4681 tctttttttg taataccggg acgccaatct tttttgtctt ttaacaaaat gatcgtttcg
     4741 atcatactaa tcggcgcatt atcggtggcc gtttcagcac gaccggcctt acctaagaca
     4801 tgtgccactt caggtacggc tttgatcaat cgatcctgta tcgtcagaat tcgattaact
     4861 tccgtaagcg aggcattggg cagtgtgacc ggcatgtaca agatcgagcc ttcatccaaa
     4921 ggcggcataa attccgatcc catatttacg atcatcggta tcgtaataat aagcgcgatg
     4981 acgttaagtg ataaagtaat tttacgatac cgtaaaaccc agtgcatgac tggctcgtac
     5041 agtttgataa aaaaatgcgt tacaggattt tccttttcgg gtcggaattt tccgcgcatc
     5101 aaaaatgtca taagtaccgg tatcagtgtc aaaacaacag aggccgacgc cagcataaca
     5161 aaggttttag taaaagcgag cggagcaaaa agtttaccct cttgaccggt aagcataaac
     5221 accggcaaaa aagaaacaag gatgatcagt tcggaaaaaa atatggcgcg cccgacttgt
     5281 ttagccgact gtagagcaat ggcctgataa tcctcggtta gcagctttcc tttagtttcc
     5341 agtgcctggg cgatgttacg ataggcattt tccaccatca cgatggatgc atccactatc
     5401 acgccgatgg cgagtatgat gccccccaga ctcataatat tggcatgtat atcaaaaatg
     5461 cgcatcaaaa taaatgacag cagaatggat acagggattt cgatgataat acgaagtatg
     5521 cttcgtgcat gaagcaaaaa cacggctacc ataagcgaga ccgtcaaggc ggcttcgaat
     5581 aaggcccgat taagcgtttc gattgattgc tcgataagca cactgcgatc atacgatggt
     5641 ttgatgacaa cgccatgcgg caatcccggt gcgatttcat ttattttttt ctttatacga
     5701 tcaatcacgg ctttcgcgtt ttcgccgctg cgcatgacta cgatcccgcc gacggcttcg
     5761 ccttcaccgt ccttttccaa tgagcctctt cgaatatcac caccgatctg cacatcagct
     5821 atttgcccaa gcaaaacagg tatcccgctg ccattatttc gtaatacgag attacgaata
     5881 tcgctctccg ttcgaatata cccttgtccg cgtacaaaat attctgcgct gctccattcg
     5941 ataatcttgc cgcctacttc attattatta cgttgaagcg cttgggtaac atccattata
     6001 tctattccaa acgcccgcat tcgtaccgga ttcaagtcta tctgatattg tttgacatgc
     6061 ccccctatac ttgccacttc ggcgacccct tcaacggagg aaagttgata cctgatatac
     6121 caatcttgta tggatcgcaa cgttcctaaa tcttgttcgc cgccctcgac cgtataccag
     6181 taaatatgac cgataccggt gccgtccggc cccaatgtag gtatcgcatc tttaggcaac
     6241 gatccgcgaa ccgtgctcaa acgctcctgg acacgcgaac gtgcaaaata gatatccgta
     6301 ttatcatcaa aaatcacaaa cacaaacgac ataccgaaca tggaactggc gcgtacagcc
     6361 ttgacgtgag caagcccttg gagtgctgta accagaggaa atgtcaattg ctcttcgaca
     6421 acttgcggcg agcgacccat ccattccgta tagacgataa cttgattttc agataaatcc
     6481 ggaattgcgt caacgctcat ttccctaaat gaataaatgc cgtataacga aatggctaca
     6541 taagctgtaa gtaccatcca acgatggcgg gcgctccaat caatgatatt ttcaatcatg
     6601 atgcgcctca cttttcatgt tagacgtacc tatggcttta taaaaatcag cttcggccat
     6661 tttactaccg gccaatgcca tgaccaaatc atctttcgtc atataaatca tccgcaacat
     6721 agacaacaac attgcggtat cagctttgcc tgtctgaaaa gaaatcatca tagattggta
     6781 agccgcttca taatgcggga gaatccgttg ttcgatttgt tcggcctgac ggcgcgatga
     6841 ttttatcctt tcgatggccg atcgcgcttc agaagtaatc atgatcttca tgttttctat
     6901 ttccatggcg ccatgtttta tatgcatgtc caattccttg atccgtgcat ccagtgaccc
     6961 tcttgaccaa ggtgcaaagg gaatattagc gctgagcatc agagaccaat aattttttcc
     7021 catgctcata tctttataca tgcctcttac catgatatcc ggaaaccatt cgcgatgtgc
     7081 cgcttttttt tcagcagtaa acatcgcgat ttcatgattc atcccctgaa tttcaggttt
     7141 tttaggcagt gtattttgta tcaatgtatc cagtactacg gaaaaattat tcgtccagtc
     7201 cacaggtatt gaatccggcc atcgaatttc attatttccc aagactccat ttaacatgga
     7261 ttccgtaacc cgacgatctt tctccaactc gaaagcctta ttctctaaag ctaatcgctc
     7321 attttcgatt tgtaatatat cggcttgtgt tatttgcccg acttcatagc gtttcaatga
     7381 taactgtaat aattgctcca atgtacgttg cgcattagcg ttattacgtc tacgttcgtt
     7441 aatcatacat aactcaaaaa aagccacacg tgcatcacgc tctatgttta attgggcgct
     7501 tgcaatcatt gtcttttgaa tttctgctgt ataatgtgcg gcctccgaca tggcctcgag
     7561 tttaccgggc caaggtatca tttgttgtat aaaataatct gtttccatat tccctttgac
     7621 cggtatagga aaattgctcg ccggcatttg aaataattcg aatcccacat ccggcgtcgg
     7681 ccatgatttt ttttgttttg cttgaaaacg atacgcttca taattgtgct gtatcgcgtg
     7741 aattttggga tgatgttgta tggcctcgcg aataagactg tctatcaata cctgcgtgtg
     7801 aacctgcggt atctccaaac ttgcgagacc gatgattagc gctatttttt ttaacgatcc
     7861 ggacataact tttcctcaat aaattgaata ccggctaaat actatgattt agcatagatt
     7921 cagtttaatc ggaaaattct aaatgcggaa tgtactaagg agagcgttgg tttctgaggc
     7981 cgtattggca ggtggatgct gatgggaaaa atcgaaaaaa gaattcttat tgtatgcttg
     8041 atcaaaggcg gtttgaaata caaaattcag taatgcatgc gtttgctgca ccgagttggt
     8101 tttgagtgtt tctataggta acgaaacacc acgctccggc aaaacgctca tcattcccgg
     8161 acaatggcat gatttatagg cagcgatttg aagcgaattg cttgtaccgg ccatagtcgt
     8221 agaagactgg caacaagaca tcgaattagt ttttgttgaa tagcatgtcc cggtaatgcg
     8281 gacattcgct aaacaaccgg tcacccaaac cagcgccaga agaagagata aaaccttttg
     8341 taggctttga ttattcatat gttaaaaata ccccggaaaa cctgcggaat caagcaattt
     8401 ccaatgggaa tcatgaatat tgttttttac gatttgtacc acattcatat cggaccactg
     8461 aggatctttt tcagcacata aacgcgtaaa tgatttgatc cattcggaaa cgccgacaat
     8521 atcatctccg ttcagctgaa tcggttttaa tgccgttgtt ttacctcgct gatctaccac
     8581 aaaatatccg agagtattcc atttattcac atgtgcgagt tgcgcacgac gaaaataatt
     8641 tcctactttc gcttcgccca tagattgccc tacgtgcatg gtagccgcat ttaatgccgc
     8701 aattactgca tcggccggta tggataccgc tttactccct aaaggagcag gcgccatgcc
     8761 tccttctttg gatgacatgg ttgacttagc atccgaacgc aacgatttgg tcagcgcatc
     8821 taaggttaac aattgttgat tgatttcaaa tgtaccgttt tgccatgaca tgagttcgtc
     8881 aatggcggca tcatcttttt tatcgtcaag cgatacttcc agcagctcgc cttttctgta
     8941 catcaatttc ccgcgacggt tttcgtgcgt tacgatcaat tcacccgtca gcgaattggc
     9001 ttcacaaaaa gcaatcacat caatcaaagg tttttcttct aattttccaa cgggcttatt
     9061 ttcacgacgc gtatccagat ctgctttcac cgcatggcct tgctgaggtt gaggtttggt
     9121 ctggtgacgt gcttcgattt tggagagaac catttgtacg cgcgcaatga cctctttgat
     9181 tcgaatcggc ttggaaatat agtcttcagc gcccaattga aggccttcga taatatgatc
     9241 gttatccgta gcggccgtca aaaaaataac cggtatctga ttataatacg ggtctttctt
     9301 taaactttgc aaaagctgaa atccgttcat catcggcata aaaatatcgc tgagtatcaa
     9361 atctacattc tgattctcta caaccttaag cgcttcagaa ccgtcggaag cggaaaaggt
     9421 tttgaacccg ttttgttgaa gggccatttc gcaaataaga cggatgctct cttcgtcgtc
     9481 aaccacaagg atgcgtttct tttcagtcat tggttttcca tatatcgttg ggtgatttcc
     9541 aataatcttt tggtttctac gggttgctca atgatttcat aaatacgcgc ctggattttg
     9601 agcgccatga tatgatgcgg ctgttctttt aataccgatg cggtgatttc caacgcatct
     9661 ttgtataact ggtaccgcat atagatggtc gcggtcaaca gcttttttaa tacagtatct
     9721 tccgttaact caatagcatc gttactttcc cgtaattcat tctcaaattc agtgcgttct
     9781 tctttattca tcacataaaa ccaaacactc tctgttgcct cattgatatt ctcattcacc
     9841 gcttccgtaa accaaaaata tttaatgccg cgctccatat tcatttcgtt acgaaacgtg
     9901 aatgaattac cgagcaccgt ctcttcaaaa gagtaatcat tttcatacga tcgtaaatgg
     9961 acaacaaatt cagacgcacc ggttatttta tcagggatat gacgccatat caattgcgct
    10021 ggaatatcta taagccgtgt attacgtgga gttattgcag ataaagaaac ctctgcatca
    10081 cttcgataaa tttgtttgga ataatgagac tgagctttaa aaaaccacga ttgtgtggcc
    10141 gactgttcgg cgaacatggc caatatttct ttacgcaatt gttttagatc aggcacatta
    10201 aattttccat tatattcttt cactgcaccg tctgcccaaa aaactttaca ataatcccct
    10261 tgcccaagat caattacatc acctttatat agtaaatccc cttgtttgat ggctttatca
    10321 cctttggctt caatattcgc gcgcaaagat ataaccatgc cgaacggttc ttttatattt
    10381 ttttgttgtg caaaaataga gccggcaatc attgtcagta ttacaagatt tttaaacgcc
    10441 ataactgatc attccctgtg attcgttagg ttttttaata tcgaaacttc cggaaaaccg
    10501 gttgttgtga tttgatttgt atttagagta aattccaatt cgcgatattc ttcatcaatg
    10561 tgagcttgat aagcaatgta ccgatccgtt aaaaaaagca cgcgttgatt ggctgaactt
    10621 cgccataaac gattaacgtt taattcccgt ttatattctc cgtccactaa tatatccaaa
    10681 gcttctaaca aagacacata aaaattaccc ttagttcgta tttcctccac agtaaaacct
    10741 gaataactca tgatggtcag attttgctta cgcaagagtt gcgcaacttc ggtaagacat
    10801 tctgcataag caaacggttc accgccactg aatgttacgc cttcaatatc tcgaaaaccg
    10861 tgagtggctt tcgaaatcat cgttacaagt tctttggcag aaaccgtatt tgttgatttt
    10921 tcaaatgata agaattccgg attccagcaa cccggacatc gtttagggca accctgaacc
    10981 catactacgc aacgctttcc gggaccgttt acctcggata tgggtaatat tttagcaacg
    11041 gaaatcattg caataaaaaa agcttattct cacgaataag ctttataata tgattgttac
    11101 tatttctcat gattcgataa taccacggcc tgttttggta atacgcccca catttttaag
    11161 atcttcatag atcgagtcca aaataccatt gataaatttt gaacttttat ccgtgctata
    11221 tcgtttactg atttcgatca tttcattaat agacaccttc ggcggtatgt cttcaaaaaa
    11281 caatatttca caaagagcca tgcgcatcaa aatacgatca atgagggcta ttcgttcaaa
    11341 ttcccaattt tgagctcttg ctcggatcag cacatccatt tccatttggt gcgcgattgt
    11401 ttgttgcaca agagtgtgaa taaattgcgt agccggttcc gtaaaattat cgcagacgtc
    11461 atcaagtact cgcttgatat tgtcttgagt catttcataa gcatacaatg cttttaaaac
    11521 cgtttcccgg gcttcgcggc gactgttttt aggcgccgtg tttttaccgg gtttcgctcc
    11581 atcatccgca agcaatgggt tcatacgttc caataatgcc aaagatcaaa ttataaattt
    11641 atttatcgtt ctatacgttt taaaaccgac tccggttgtg tcacataatg acttttgata
    11701 tgcccgattt gacgaatgcg ctcttcggcg atgcgattgg aagcttcatg cgttgagata
    11761 ttctctcttt cggcaagctc caatatcttt ttcataatat cgcaaatgcc tttagcttgt
    11821 gccaatgctt tttcctgatt gtatccttca agttcgttat aaacattgat aagccctccg
    11881 gcattgatca cataatccgg ggcataagtt atttttttcg ccaaaagcat agcaccgtgt
    11941 cgggcctcat cctgaagttg attattggcc gctccggcga taatcttgca tttcagtcgc
    12001 ccgatagtgt catcattgat aatcgcgccc atcgcgcacg gtgaaaaaac atcaacgtcc
    12061 gcatcgtata tcttatccga atcaacatac tcagcaccga attcgctgac cacacgtttg
    12121 acgcgttctc catccacatc ggttacgacg agttttgcac cttcatcatg gagatgtcgc
    12181 accagataat atccgacatg acctaagcct tgtacggcga ttcgtaaacc tttcaacgat
    12241 tgtgtaccaa aaactttttc tacgcaagct ttgagaccgc gatacgttcc taaagccgtt
    12301 accggcgaag gatcaccgct gccgccttga gcacgtgata tgccggttac ataccgggtt
    12361 tccattcgaa tccattccat gtccgttacg ctcgttccca catcttcggc cgtaatatag
    12421 cgacctccta atccttgaat aaaacggcca aatgtacgaa acaaaatttc gttcttatgt
    12481 tttttcggat caccgatgat aacggccttg cctccaccta gattcaatcc ggctacggcc
    12541 gctttgtagg acataccgcg ggataaccga agtacgtcat tgagcgcctc ttccgtcgtc
    12601 gcataaggat acattcggca gcctccaaga gcaggcccta gagttgtatc gtgaatcgct
    12661 acaatggcac gaaggccgct tgctttatcg ctaaagaaaa cgacttgttc gtgttccttt
    12721 tcttctatca tttcaaaagt gcgcatgacg tattctttct taaattccgc gcgagattag
    12781 cctaaaacgt tacgagcaat tacgacgcgc tgaatttcgg aagtgccttc atagatttcg
    12841 gtgatcttgg catcacgcat aaaacgttct accgggtatt ctttggtgta accataacca
    12901 ccgtgcattt gtaccgcttg tgtagtaacc cacattgcgg tttcagaggc aaaaagcttc
    12961 gccatggccg cttctttacc aaacttctga ccttgatctt tcagagtgca tgcgcggaat
    13021 aacaataatt tggcggcatc aatacgcatg gccatatcgg cgattttaaa ttcgttggct
    13081 tgaaatttgg ctatcggttg cccaaactgt tcgcgggctt tggtatattt cacagtttct
    13141 tccaatgctg cttctgcaat gccgatcgct tgtgcggcaa tgccgatacg accgccatcc
    13201 aaagtttcca tagcgatttt gaaaccgagg ccttcctgac cgagaagatt ctctttgggt
    13261 actttgcagt tttcgaatac gagttctgtc gtgtccgaag cgcgaatacc cagtttattt
    13321 tcttttttac cgactttaaa acccggaaat gatttatcaa cgacgaacgc gctgatgcct
    13381 ttggtcttgt tggatttgtc cgtacgtata aaacatacca cgtaatcgga atgataaccg
    13441 ttggtcaccc ataacttagt accgttgacg atgtagtgat cacctttgtt ttctgcaaaa
    13501 caaatcagac ctgcagcatc gctgccggat acggcttccg agagcgaata cgcaccaagt
    13561 gcaccgcccg ttgcaagcgg tttcagatac ttttcttttt gcgcatcggt tccgtatttc
    13621 tcgatattaa aacaaactaa agagttgttg accgatgcga taacagccgc cgaagcgtcg
    13681 gctttggcta attccataat cacatgaacg tacgaaattg tatccaaacc ggcgccgccc
    13741 catttatcat tgaccatcat acccatgaag ccaagttcgc ccatttgttt gatcacatct
    13801 ttaggaaaaa actgtttttc atcacgttcg gcagcatgcg gagccaaatg ttcctgtgca
    13861 aaatcgcgag ccgtctggcg aatcatttcc tgttcttcag agagtttaaa atccatggca
    13921 agatccttat cttaaacgta tatgatggta ttgcaataac gccgcgaata tagagaagca
    13981 gtcgagaata agcaagaatt tctttgctgc tccatggcgc ttcggcaaca aaaaagccgc
    14041 catttcaggc ggcttgatgt aaaaaatcat attgccagtt ctttatcggc aacccaatag
    14101 ttaaaatgtt tttgccagta ttcaacccat tccgtcgtga ttttggaacc gcgttgatgc
    14161 tcattccaga tttgtgcggc gatttcataa ttggtttttt gttctttggt cttccatccc
    14221 aattcatacg cttcgccggc taattttaca cccggacggt tattcccgct tttgatttcc
    14281 aagcaagctt tacgttgcat gaagtgcgcc atgtgtttgg ttg
//
LOCUS       DAOQNQ010000052        14194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1523_length_14194_cov_5.467713, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000052 DAOQNQ010000000
VERSION     DAOQNQ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14194
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1523_length_14194_cov_5.467713"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PK754_05210"
     CDS             complement(<1..406)
                     /locus_tag="PK754_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNB08934.1"
                     /translation="MKSKTIKKNKPIVKKNSVKTQPKISESKNQTPYRALGLAPDDLL
                     RMYRLMKLTRQFDQGIIRLYRQNKMIGGAYSGWGNEATAVGSAYPLLPHDYLYPMHRD
                     IGAHFTKGQSAKILMLNHLARAEGPTRGVDGTG"
     gene            complement(447..1997)
                     /locus_tag="PK754_05215"
     CDS             complement(447..1997)
                     /locus_tag="PK754_05215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB08935.1"
                     /translation="MGVLALAELELKEFEKTICARQLTIDDYESVTELQIRSFPGMKP
                     WSYDQFESQIRLFAEGQIGIEYNGLLVASSSSLILDFDPYKDWHSWKEISDNGYIRNH
                     IVNGNTLYGIEIMVDPGYRGLRLARRLYEARKSLARKYNLRSIILGGRIPGYDKFADQ
                     MDVKSYVEKVSSKVLVDPVLTTQIANGFVLKRIIPNYLMSDTESRGYATFLEWTNLDY
                     VPDFAGEKQKTIVRISAVQYQMRHIRDFEDFAKQCEYFVDVASDYRSDFILFPEMFTM
                     QLLTFLPNLRPGLAVRKLAEYTPKYLELFNHLSVKYNINIIGGSHFTVENEDLYNISY
                     LFRRDGTIGKQYKLHITPSERKWWGVKAGHKLEVFDTDCGKISIQICYDIEFPELTRV
                     AVEKGAQIVFVPFCTDERYAYLRVRYCAQARCVENQIYTVIAGSVGNLPYVENLDIHY
                     AQSAIFTPSDIPFTRDAIQAEATPNIETVIIDEVNLETLRHNRQSGSVLNWQDRRTDL
                     FKVNYQGS"
     gene            complement(1985..2509)
                     /locus_tag="PK754_05220"
     CDS             complement(1985..2509)
                     /locus_tag="PK754_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNB08936.1"
                     /translation="MAKFVVHIAKEYLKFSSAHFTIFSDHALEMLHGHNYYVTLDAEF
                     RDSANGITIDFKHQKKIMEALCDELDEKVLLPSESPYLKIEKITDNFRVVFHGEGFTK
                     EYQFPCEDVQILPVNNISSEMLAKQLCESFCEKLMQTWQHLKPGSDISEVLLAVHVGV
                     EETRGQSVKYVWEY"
     gene            complement(2537..3886)
                     /locus_tag="PK754_05225"
     CDS             complement(2537..3886)
                     /locus_tag="PK754_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNB08937.1"
                     /translation="MQELSNENNVHLVKRVASVVVTIIIMLALRYSTLITESDPSVFS
                     VTLLGLMLVLSFNIGKLLSRLNLPTLSIYLITGILCGPFVLGFLSSSTIDNLKFIDTL
                     ALGLIAFIAGGELRLKELLRMKKVLVSISLMETLMVFIFCAVGFIVLTPLMPFTQSQT
                     FLLNSLIALIMAALLVANSPAVTIGIIGEYKSKGLLTETILGSVIFKDIIVIVLFATV
                     TTFVMISLDSTAEWSMMVFIKTIGYEIGMSIVAGLGVGFLVYLYIRFIGEQPVLFVIG
                     AAFMSYEIARQFHLEVLLVGVSAGFAVQNFTKQGEALVEHVEESLPIVYPIFFAIAGA
                     KINLPLMLEMWYLALFLAVIRTLGIYFGVRKGARMASAAESVQKYAWMGFVSQAGVAL
                     GLATIVNYKFPEWGGYLQTILLSVIGINQLVGPILLQKALDKAGEINKPMPQGSILN"
     gene            4019..4663
                     /locus_tag="PK754_05230"
     CDS             4019..4663
                     /locus_tag="PK754_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08938.1"
                     /translation="MPTNPIEAAIRNHMQIDHATGSIALSDFFTPTARRIGIADYDFF
                     EALFSVFSENSTPDQTRERFYQFGADWGNSFLERLKTQLAQAGGEHASEPQALLKDEF
                     VEHLNSNFSYLGVGQFRILEGNKFYIIDLKNPWRPSDEHKQIYASTSFAGFFSTLFSG
                     IAGRALSSTFLSNTPEQWRFALSTTEVIEDIRTQINAGLTETDILTQYHNQHLL"
     gene            4671..5504
                     /locus_tag="PK754_05235"
     CDS             4671..5504
                     /locus_tag="PK754_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB08939.1"
                     /translation="MGQIFSIASQKGGVGKTTTALSLGIAFSRLNKKTLIIDADPQGG
                     ISFCLDRYYDMRRDQDQGRNGLYQVFCGAMDIAEAAQPSMIENLSVIDCGISARSSDI
                     ELYEDATRATGLFREAVIQAAQHYDTVLIDCPPGIGLVSAGALAASDYVIIPVQSEPL
                     SLRTLPQLLRQLIAIKKEHNPDLAMAGILLTMYDHQSPASEMVAQQIRDFFEEDIVFK
                     QAIPRDPVINLLFAGHENITSVLTEIEAQSVAFQAYQALASEIAIQFSETPLRLNSIS
                     H"
     gene            5527..6186
                     /locus_tag="PK754_05240"
     CDS             5527..6186
                     /locus_tag="PK754_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08940.1"
                     /translation="METTSYEFTLPENYTAFSPVSAEIFVQNTNRSFAVDTVFLKALQ
                     QSFARLYAAHDQDALYRTGVQWGNSVYAKIETLALTMHPDAKSIREIPMPDFHRLFTN
                     HLAALGWGNFELKRRDDFLFVDLYHSLWVEMLQSQSQAHGSKPHTSCALYAGFFAGIF
                     SRISNMPLACIEITCKNEGYEHCSFLLDNEETIAMVKARISGKTTPLDAFEKVKKEFE
                     G"
     gene            6192..6617
                     /locus_tag="PK754_05245"
     CDS             6192..6617
                     /locus_tag="PK754_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08941.1"
                     /translation="MNAKSKPFEETGLAAHLLSQFTSMRRTTKKSVQTAPALANDFFS
                     KDILTMLDFLQPLGTDIIATTASPPSHTIESFFNSSLSTRRAFRSAPVKTEPEWTPDE
                     VISIDMVETKANPTTSPLAQEEAPARPRTIEEFINGLFQ"
     gene            complement(6700..7224)
                     /gene="tpx"
                     /locus_tag="PK754_05250"
     CDS             complement(6700..7224)
                     /gene="tpx"
                     /locus_tag="PK754_05250"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014369304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNB08942.1"
                     /translation="MKERAGHLSIKGKPLTWLGEEIKAGQKAPDYRVRTGFGPEATVT
                     LADSKGKIRIISVAPSLDTGVCEMQAIRFNQEVEKLGADIEVHQITVDLPPAMTRFCN
                     TKLNGSVKIKTFSDYAERSFGQAYGFLIKEWQTLGRGIVIIDKNDTVAYVEYCPKIEE
                     LPNFDVALAALKGL"
     gene            complement(7264..7926)
                     /locus_tag="PK754_05255"
     CDS             complement(7264..7926)
                     /locus_tag="PK754_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNB08943.1"
                     /translation="MARAGSIIRSSSGKDIFTRRDKDFFEFFGQLESVENKFNAYLTR
                     FKYPKREKYLIRKAYDFSSLAHKGQLRNDGAPYIIHPIRVASILLHELSVTSADMICS
                     ALLHDVIEDCGITENEIKNNFNESVASMVRTLTKNPNLSNAKRVYFEAIMQAEDHIRL
                     VKLCDRLDNLRSLRLIRNKAKTTRYIKETEGQYLPIAAEISPYLFRELRFEIQYQRTR
                     YR"
     gene            complement(7996..8394)
                     /locus_tag="PK754_05260"
     CDS             complement(7996..8394)
                     /locus_tag="PK754_05260"
                     /inference="COORDINATES: protein motif:HMM:NF021486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HNB08944.1"
                     /translation="MSGSVIHNKHYSPEEARILLHDIRVKINTLVALKKHLDEINYNI
                     YKHEYFGGLGPNGSKFHPEELMTLVDILRSLEKQGVIIKSIDEGLIDFPAIRANGEEV
                     YLCWKLGEEDIFYWHTIRDGFQGRRPLSAF"
     gene            complement(8396..8626)
                     /locus_tag="PK754_05265"
     CDS             complement(8396..8626)
                     /locus_tag="PK754_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08945.1"
                     /translation="MFDTYSSKVELAQSDFNEIAEQYFQNYLSSWCDHNKIDDASRKA
                     MVTVLSDYSNFFFDALMQSTIEQHADRTELKN"
     gene            8719..9591
                     /locus_tag="PK754_05270"
     CDS             8719..9591
                     /locus_tag="PK754_05270"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HNB08946.1"
                     /translation="MNLSQKNNWRVGILGAGRVGSSIALALSGNKIPILSVVSATSHS
                     AAVLAKKINCKIFSNKIEDLRTCNVIIVSVPDRQLKAITKRVMQIRFIHNSVLLIHTS
                     GMYGATIWNQRTSENKEVKFWTAAMHPLQTFVTRMDTSPEIFKTYFALDGKNKPVSIV
                     KHIVKIMGGKSIMISDKDRATYHASAVIASNFLVALSHASTELLRQIYPQSSGLEKLF
                     LPIQQQTLANISKHGAAASLTGPAMRGDKQTIRLHKKAIQKHSKDILKIYTTLTSYCI
                     ALSEKRQRGKINEP"
     gene            9581..9985
                     /locus_tag="PK754_05275"
     CDS             9581..9985
                     /locus_tag="PK754_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08947.1"
                     /translation="MNRSLKLLFFAALVMNLSCREPLVNSEDAKLENNLVGTWILVST
                     FPETPGEIQLTFRDDLSLHVYSKTATNPPVVRDEEWHFFVDDKQIVMFRDEIDTNGQP
                     YTQYDYYPIKSLTHKELVFVNLERIQQTYARN"
     gene            9985..10521
                     /gene="ruvC"
                     /locus_tag="PK754_05280"
     CDS             9985..10521
                     /gene="ruvC"
                     /locus_tag="PK754_05280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNB08948.1"
                     /translation="MVILGIDPGTNTTGFGIITWERNKVNWVASGTIELPEKETLARK
                     LELIYDHVVDLAHTYKPNECAIENVFYSKNVKSALKLGHARAAAMLGALHKAVHISEY
                     SPKDMKMAVVGNGNASKEQVQYMVKMVLGITKEMSFDESDALGLAICRAQRIKTPRAS
                     VSSWKAYIKAHPDRVKVK"
     gene            complement(10564..>14194)
                     /locus_tag="PK754_05285"
     CDS             complement(10564..>14194)
                     /locus_tag="PK754_05285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNB08949.1"
                     /translation="SVSVQIALSTNIQNPAVSGNYQLSVKTVNSGGATIDNAQASNSY
                     NIAAATTTVSSSSVALSNTTAAQTNVSYTLGFTLGSYGRMLSGSSVFKVKFPSGTGYG
                     TLTGTVNGTSASTPTRSGDTVSVILPSTVSLSNSASVTLVIGGITNPANGSYTISLAT
                     SVEASYVVSQSFTIGGTAVTISSVTLGNSGVNQTSAYTINCTGVSNLNTGDIVRVTFP
                     EGTTVPSSIAAANVTFTGGTGQTVTSVATNTSSRFADITVGSNNKTFAAIVFASGAGI
                     VNPSVPSTTFYKVNVVTTTNTQPATSPAYTVSSNTTSVTADTVVVTSNVVSQTNAIYE
                     VYFTTGAFGKLAGGTSAGSDSIRIRFTSGGVVVPSSITAANVTINGVSSTGVRVVTSG
                     ANGEVKIEMPSGASVPASTQSKVTFSASAGLTNGGSAGSYQVQVATDAENTLSTKTSN
                     MVFAASSALAVTQVTPSPSTINATAAYTIKFTTGSSGALAINDSILITFPSNTYLPGT
                     ISSSLITVNGSQLAASAVGSNNVLRIRNPVTINNVANVTIAISSSAGILNPTGVSTSY
                     SLTVTTKTSGGTVVEGPTTSPQYTTTATSTTITTPSVTLSSSTPSASSNYTIAFNTGT
                     NGRLRSTTDSVSIVFPSGSTVPASPTVTINTVSASAYGTGLKVTAAIPASVSIGNSGS
                     ASVVINGITNPTAGNYTLSAYTTVESGSITSPSYTINNAPLLTGVSLAFTPNTDTVNM
                     AGGDSIYFTTGTGGALTSGSSTITVVFPNNTVIPASITASNVTVNSVAASVVATNSGT
                     RTVTVTSPTNVNASTNVTLFFATACGIINPSVAGTYAVQVSTTPQPNLTTSSNYTVKA
                     ATTTVTNFTMTLTQSGTSDGPNEFGRYNYTFKTGLRGKLLSGTSTISLLLPYDATFTT
                     GTPTVSTVTVNGTAAAALRLGLSTLNEDTLEVTVPASVTIGNQTSVTVIIDSTSGLRN
                     ASTASALTYKVFTSVETGVVGGDATLPVELAFFDVVQNGNKAVLKWRTESEINNAYWL
                     VERAESNFIENEDDLQGKTSHLQYTRVAEITGQGTKNTSTDYILNDDKVEIGKTYVYR
                     LMDVSYDGVLSIHQEKKIIIETPQVFDVFPNYPNPFNPSTLIRFQIPSESHVVVTIYN
                     LLGQEVKELVNKDLPAGMHTMQWNGTNNRNSLVASGVYLYRVTATSLQNKSKYSRTHR
                     MTLLK"
BASE COUNT         4054 a         3230 c         2918 g         3992 t
ORIGIN      
        1 gtcccgtccc atccacaccg cgtgtcgggc cttctgcacg ggcaagatgg ttgagcatca
       61 aaattttggc tgactggcct ttggtaaaat gtgcgccgat atcacgatgc atcgggtaca
      121 aataatcgtg cggcaaaagc ggatacgcgc tacctacggc cgtcgcttca ttaccccatc
      181 cgctatacgc tccgccgatc attttattct gacggtacag acgtataatg ccctgatcaa
      241 actggcgtgt cagtttcatc aacctgtaca tacgcagtaa atcatcgggc gccaagccca
      301 aagctctgta tggggtttgg ttttttgatt cgcttatctt aggttgcgtt tttacgctat
      361 tctttttcac aattggttta ttctttttta tcgtcttgga tttcatgaat tcctctatat
      421 gatgaactat ttaaataatg aagtaattac gatccttgat aattaacttt gaacaagtct
      481 gttcgacgat cttgccagtt taatacactt ccagactgtc ggttgtgacg aagtgtttca
      541 agattcactt catcaataat gaccgtttcg atattcggcg ttgcttcagc ctgaatcgcg
      601 tcgcgcgtaa acggtatatc cgacggcgta aaaatagccg attgtgcata atgtatgtct
      661 aaattttcca catacggcaa attcccgaca ctgccggcga tcaccgtata aatttgattt
      721 tcaacacatc gagcttgtgc acagtagcgt acgcgcaggt atgcatatcg ttcatcagta
      781 caaaagggaa caaaaacaat ctgggctcct ttttcaaccg ctactcgcgt cagttcgggg
      841 aactcaatgt cgtaacatat ctgaatcgat attttaccgc aatccgtatc aaaaacctct
      901 aacttatgtc ccgctttcac accccaccat ttgcgctcac tgggcgtaat gtgaagttta
      961 tactgtttcc cgatagttcc gtcacggcga aataaatagg agatattgta caaatcttca
     1021 ttttctaccg taaaatgcga tccgccaatg atattaatat tatattttac agacaagtgg
     1081 ttgaataatt caagatattt gggcgtgtac tctgctaatt tccgtacggc cagcccgggg
     1141 cgcagattgg gtagaaatgt caacagctgc atggtgaaca tttccggaaa aagtatgaaa
     1201 tcagatctgt aatctgatgc cacatcaaca aagtattcgc attgttttgc aaaatcttca
     1261 aaatcacgga tatgacgcat ttgatactgt acggcagaaa tccgtacgat cgtcttttgt
     1321 ttctcacctg caaaatcggg cacataatca agattggtcc attccaaaaa agtagcataa
     1381 cctcttgatt cggtatccga catcagataa ttgggtataa tgcgtttgag tacaaatcca
     1441 tttgcgattt gcgttgtcaa aaccggatcg acaagaactt tactacttac tttttccaca
     1501 taagatttga catccatctg atccgcaaat ttatcataac caggtattcg ccccccaaga
     1561 ataatgcttc ttaggttata ttttcttgcc agcgatttac gtgcttcata taaacgacgc
     1621 gcaaggcgca atcctcgata tcctggatcc accataattt caatgccata cagcgtatta
     1681 ccattcacga tatgattgcg aatataccca ttgtcagaaa tttccttcca actgtgccag
     1741 tctttgtaag gatcaaaatc aagaatgagg ctcgaagaag atgcgacaag taatccgttg
     1801 tattcgatac ctatctgccc ttccgcaaat agtctaatct gactttcaaa ctgatcatac
     1861 gaccatggtt tcatacccgg gaatgatcga atttgcaatt ctgtcacgga ttcgtaatcg
     1921 tctattgtaa gctgacgagc acaaatcgtc ttctcaaatt cttttaattc tagttccgcc
     1981 aaggctagta ctcccataca tattttacag attggccacg cgtctcttcg acgccaacat
     2041 gtacagcaag taacacttca gaaatatcac taccaggctt aaggtgttgc catgtctgca
     2101 taagcttttc gcaaaaagac tcacatagtt gtttggcaag catttcagaa ctgatattat
     2161 tcaccggaag aatttgtaca tcttcgcacg gaaattgata ttccttagta aaaccttcac
     2221 catgaaaaac aacacgaaaa ttatctgtta ttttttcaat ttttaaatat ggcgattccg
     2281 atggtaataa aactttttca tccagttcgt cacacaaggc ttccatgatt tttttttgat
     2341 gtttaaaatc tatagtgata ccgttagcgc tgtcacgaaa ctcggcatct aaagtcacat
     2401 aataattatg cccatgcaac atttccagag catgatcgct gaaaatagta aaatgtgccg
     2461 agctgaattt taaatattct ttggcgatat gtacgacaaa tttagccata ataatttata
     2521 acaatctttt cttctattaa tttaaaatag aaccttgagg cattggttta tttatttctc
     2581 cggctttatc aagcgctttt tgaagcaaaa ttggtcccac tagttgattt attccaataa
     2641 cagacaataa aattgtttgt aaataacctc cccactccgg gaatttgtaa ttaacgattg
     2701 tcgctaaccc taatgctaca cccgcttgcg aaacaaagcc catccatgcg tatttttgaa
     2761 cagattcagc tgctgatgcc atacgagcac cttttcgaac accaaaataa attcctaaag
     2821 ttcgaatcac tgctaaaaac agagcaagat accacatttc aagcattaac ggcaaattaa
     2881 tcttcgctcc tgcgattgca aaaaaaatag gataaacgat tggaaggctt tcctcaacat
     2941 gctcgactaa tgcctcccct tgtttggtaa aattttgaac tgcaaaacca gcgctaacac
     3001 cgaccaaaag aacttctaga tgaaattggc gcgctatttc gtaggacata aatgccgcgc
     3061 cgattacaaa taaaacgggt tgttctccga taaatcgtat gtacaaatac acaagaaatc
     3121 caacacccaa tccggccacg attgacatcc cgatttcata tcctatcgtc tttatgaaaa
     3181 ccatcatgct ccactccgcg gtcgaatcga gcgaaatcat tacaaaggtt gtaacggttg
     3241 caaacaagac aataacaata atatctttaa aaataaccga gcctaaaatt gtttccgtca
     3301 agagtccctt acttttatat tcgccgatga ttcctatcgt tacagccggc gaattggcca
     3361 ctaacagtgc ggccatgatc aatgcaatga gactgtttaa aagaaaagtt tgggattgtg
     3421 taaaaggcat taatggggtt agtacaataa aaccgactgc acaaaatata aaaaccatca
     3481 atgtttccat taacgaaatg ctaactaaca ctttcttcat tcttaataat tcttttaacc
     3541 gcaattcacc tcctgctata aaagctatta gcccgagcgc aagcgtatct ataaacttca
     3601 ggttatcaat ggtagaagaa ctcaaaaaac caagtacaaa cggcccacat aaaatccccg
     3661 ttatcaggta aatggataat gtaggaagat taagtctgga aagtaatttg ccgatattaa
     3721 acgaaagtac gagcattaaa cccaacaatg tcaccgaaaa aacggacgga tccgattcag
     3781 ttatcaatgt agaataacgc aaagccagca tgatgatgat cgtcaccaca acactggcca
     3841 cacgcttcac aagatgaaca ttattttcgt ttgataactc ttgcatgcac taatttcttt
     3901 tttctaaagt attaatttta ctcaaaaata caaaagattt tcgcgttgct ttccatcgaa
     3961 aaatcaatta gtttgccgca ttaacgcgtt ttacattatc gggtaataaa tcgaactcat
     4021 gcctacgaac cccattgaag cggccatacg aaatcacatg cagatagacc atgcaaccgg
     4081 ctccattgct ctttcggact tttttacgcc gacggcacga cgtatcggca tcgccgatta
     4141 tgattttttt gaagctctat tttctgtttt ttctgaaaat tcaacgcctg atcaaacacg
     4201 cgaacgtttt tatcagtttg gcgctgattg gggtaattct tttctggaac ggttgaaaac
     4261 tcaattggca caggccggcg gtgagcatgc atcagaacct caagcgctgc taaaagacga
     4321 atttgtcgag catctcaatt ctaatttcag ttatttaggc gttggccagt ttcgtatact
     4381 ggaagggaat aaattttaca tcattgatct gaaaaaccca tggcgcccgt cggacgagca
     4441 caaacaaata tatgcctcga cgagttttgc cggttttttt tcaacccttt tttctggtat
     4501 cgccggtcgc gcgcttagca gcacattttt atccaataca cctgaacaat ggcggtttgc
     4561 tctcagcaca accgaagtga tcgaagatat tcgcacgcag atcaatgccg gtcttaccga
     4621 aaccgatata ttgacgcagt atcataatca acatctcctc tgatcaatac atgggccaga
     4681 ttttcagcat agccagtcaa aaaggtggtg tcggcaaaac cacgacagcc ttgagcctcg
     4741 gcatagcttt ttcacggtta aataaaaaaa cactgatcat tgatgccgat ccacaaggcg
     4801 gtatctcgtt ttgtctggat cgctactacg atatgcgtcg cgatcaggat caggggcgaa
     4861 acggattgta tcaggtcttc tgcggggcta tggatatcgc cgaagcagct caaccgtcca
     4921 tgatcgaaaa tctttctgtc attgattgcg gcatttctgc acgcagctcc gacatcgagc
     4981 tttacgagga tgctacgcgg gccaccggtt tattccgcga agcggtcata caagccgctc
     5041 agcactacga tacggtgctt attgattgcc ctccgggtat cggccttgtc tccgcaggcg
     5101 cactcgccgc ttcggactat gtcatcatac cggtccagag cgaaccgctc agcctgcgca
     5161 ccttacccca gttactgcgc caactcatcg cgatcaaaaa ggaacataac cccgatcttg
     5221 ccatggcggg tatcctgctg actatgtacg atcatcaaag tccggcttcc gaaatggtcg
     5281 cgcaacaaat acgagatttt tttgaagagg atatcgtttt taaacaggcc ataccgcgcg
     5341 accctgttat caatttgctt tttgcaggtc acgaaaatat tacttcggta ttgacggaaa
     5401 tcgaagcgca atccgtcgct tttcaggcct atcaagcctt agcatcggaa atcgccattc
     5461 aattttctga aaccccattg cggttaaact cgatatccca ttaaccgctg caaccaaacg
     5521 gaaattatgg aaactacatc gtacgaattt acattgccgg aaaactacac ggctttttct
     5581 cccgtaagcg ctgaaatttt cgttcaaaac acaaatcgga gttttgccgt cgataccgtt
     5641 ttcctcaaag cattacaaca gtctttcgcg cgcctgtatg cagcgcacga tcaggatgcc
     5701 ctctaccgta ccggcgtaca atggggcaac tcggtttacg ccaaaattga aacgctagcg
     5761 ctgaccatgc accctgatgc gaaatccatt cgtgaaatcc ccatgccgga ttttcatcgt
     5821 ctttttacca atcatcttgc agcgttgggt tggggcaatt ttgaacttaa acgtcgcgac
     5881 gattttctat ttgttgattt ataccattcg ttatgggtgg aaatgttgca atcgcagtcg
     5941 caggcgcatg gttccaaacc tcacacgtcg tgcgcattat acgccggttt ttttgccggt
     6001 attttttccc gcatatccaa tatgcccctg gcttgcatcg agattacctg caaaaacgaa
     6061 ggttacgagc attgttcctt tttgctcgat aatgaggaaa ccatcgcgat ggtcaaagca
     6121 cgcatttcag gtaaaaccac gccgctggac gcgtttgaaa aagtcaaaaa agaattcgaa
     6181 ggttgaacgg aatgaatgct aaatcaaaac cgtttgaaga gaccggccta gcggcccatc
     6241 tcctcagtca atttacatcc atgcggcgta cgactaaaaa gtcagtacaa accgcaccgg
     6301 ctttggcgaa tgactttttc agtaaagata ttcttactat gctggatttt ttgcagcctt
     6361 taggtacgga tatcatcgct acgaccgcat caccgccgag ccataccatc gaatcgtttt
     6421 tcaattcatc gctgagcaca cgtcgtgcat ttcgcagtgc acctgtgaaa acagaaccgg
     6481 agtggactcc cgatgaggtt atttctattg atatggtaga aacaaaagca aaccccacaa
     6541 cttccccttt ggcccaggag gaagcgcctg cccgtccgcg gactatcgaa gagtttatta
     6601 acgggttatt tcaataatta attatttgaa gcgtgattga ttatggctat aaaaaaacct
     6661 ccgttgtagc ggaggttttt ttatgatata aacagatgat tacaatcctt ttaacgcggc
     6721 tagcgctaca tcaaagttgg gcaactcttc gattttcggg caatactcca catacgcgac
     6781 ggtatcgttt ttatctatga tgacgatacc acgccctaac gtttgccatt ctttgatcaa
     6841 aaaaccatac gcctgaccga acgagcgctc cgcatagtcc gaaaaagttt tgatcttcac
     6901 actgccgttt aatttggtat tacaaaagcg tgtcatggcc ggcggcaaat caaccgtgat
     6961 ctgatgcact tcgatatcgg cgcctaattt ttccacttct tggttgaacc gaatggcttg
     7021 catttcacat acaccggtat ccagtgaagg cgcgaccgag ataattcgga ttttaccttt
     7081 ggaatcagct aacgtaacgg ttgcttcggg gccaaacccc gtacgcactc tataatcggg
     7141 tgccttttgc ccggctttga tttcttcgcc taaccatgta agaggttttc ctttgatgct
     7201 gagatgtcct gcgcgttctt tcattgcaac tccatattta gtgaaattcg tttatatgag
     7261 tatttatcgg taacgtgttc gttgatattg tatttcaaat cgtaattcac gaaaaagata
     7321 cgggctgatc tccgccgcta tgggcagata ttgcccttca gtttctttaa tgtagcgggt
     7381 tgttttggct ttattacgta ttaaccgtag tgaacgaagg ttatctaagc ggtcacaaag
     7441 ctttacaagg cgtatgtgat cttcggcctg catgatcgct tcaaaatata cgcgcttagc
     7501 attactgagg ttcggatttt tggttagtgt gcgaaccatc gaggctaccg actcattaaa
     7561 attatttttt atctcgtttt ccgtgatgcc gcaatcttcg atcacatcgt gcaaaagcgc
     7621 agaacatatc atatccgccg aagtaactga caattcatgc aaaagtatgg acgccacacg
     7681 aatcggatgg atgatatagg gcgccccgtc gttgcgcaat tgtcctttgt gagccagtga
     7741 tgaaaagtcg taggctttgc gaataagata tttttcacgc ttgggatatt tgaaccgcgt
     7801 gagataggcg ttaaacttat tttctaccga ttcgagttgc ccaaaaaatt cgaaaaaatc
     7861 tttatctcgc cgcgtaaaaa tatcttttcc ggaggacgag cgtattatcg aaccggcacg
     7921 cgccatacca aacctttgat tagacccgaa ggcaaaagga acgggtaaat ttgtagatgc
     7981 gataaacttg ttaaattaaa aagcagataa cgggcgtcgc ccttgaaaac catcgcggat
     8041 cgtatgccaa taaaaaatat cttcttcacc taatttccag cacaaataca cttcttcccc
     8101 gttggctcga attgccggaa aatcaataag cccttcatcg atactcttaa tgatgacacc
     8161 ctgcttttca agtgatcgta gtatatcaac caacgtcatg agttcttccg gatgaaactt
     8221 ggacccattg ggacctaatc ccccaaaata ttcgtgtttg tagatattat aattgatctc
     8281 atccagatgc tttttgagcg caacgagtgt attaattttg acgcgtatat cgtgtaagag
     8341 aattcgcgct tcctccgggg aatagtgttt attatgtata acgcttccgg acatattaat
     8401 ttttcaattc agttctatcg gcatgttgct ctatggtgga ttgcattaat gcgtcaaaaa
     8461 agaaattaga ataatccgac agtacggtca ccatggcttt gcgtgatgcg tcgtctattt
     8521 tattatgatc acaccatgac gataaatagt tctgaaaata ctgttctgca atttcattaa
     8581 aatcactttg agccaattca actttcgaag aataggtatc gaacataaat aaacgcctcc
     8641 gatgaatgat gcgaatgtaa accatgcctg ttcgtaaatc aagattgttt ttttatataa
     8701 tgctcagtaa tttcatcaat gaacctatcg caaaaaaaca actggcgtgt cggcatttta
     8761 ggagccgggc gtgtgggttc atcgatcgcg ttggctttgt cggggaataa aatcccgata
     8821 ttaagcgttg tatcggctac atctcatagt gctgctgttt tggccaaaaa aataaattgt
     8881 aaaatattca gtaataaaat agaagattta cgtacttgta atgtaatcat agtttccgta
     8941 cccgaccggc agcttaaagc gataacaaaa cgtgttatgc agattcgttt tattcataat
     9001 tccgtgttac ttatccacac gtccggcatg tatggcgcaa caatttggaa tcaacgaact
     9061 tctgaaaata aagaagtaaa gttttggacg gccgctatgc acccattaca aacttttgta
     9121 acacgaatgg atacatcgcc tgaaatattt aaaacatatt ttgcactcga cggaaaaaac
     9181 aaaccggtga gtatcgtcaa acatatagta aaaataatgg gtggaaaatc tattatgata
     9241 tccgataaag atcgcgcaac gtatcatgcc tcggccgtca tcgcatccaa ttttttagta
     9301 gctttatcac atgcatccac cgaacttctc cggcaaatct atcctcaatc aagcggttta
     9361 gaaaaacttt ttttacctat tcaacaacag acgttagcca atataagcaa acatggtgca
     9421 gcggcttcat taaccggccc ggccatgcgc ggtgacaaac aaaccattcg acttcataaa
     9481 aaagcgatcc aaaagcattc caaagatatt cttaaaatat atactacgct tacttcatac
     9541 tgtatcgctt tatccgaaaa acgccaaagg ggaaaaatta atgaaccgta gtttgaagct
     9601 actttttttt gcggccttgg taatgaacct cagttgccga gagcctttag tcaacagcga
     9661 agatgctaaa cttgaaaaca atttggtcgg cacttggata ctagtgtcca cctttccaga
     9721 aacgccgggc gaaatccaac tcacttttcg agacgattta tcgcttcacg tatatagcaa
     9781 aacggctacg aatccgcctg tggtacgtga tgaagaatgg catttttttg ttgacgacaa
     9841 acaaattgta atgtttcgag atgaaattga tacgaacggc caaccctata cacaatacga
     9901 ttactacccg atcaagtcgc ttacacacaa agaacttgtg tttgttaact tagagcgaat
     9961 ccagcaaacc tacgcaagga actgatggtc atacttggca ttgatcccgg taccaacacc
    10021 accgggtttg gaatcatcac ctgggagcgt aacaaagtca attgggttgc aagcggcacg
    10081 atcgaacttc cagagaaaga gacattagct cgtaagcttg aattgatata cgaccatgtc
    10141 gttgatttgg cacatactta caaaccgaac gaatgcgcca tagaaaatgt gttttacagt
    10201 aaaaatgtaa aatccgcatt aaaactaggc catgcgcggg ctgccgccat gcttggcgcg
    10261 ttacataaag cagttcatat cagcgagtac tcccccaaag atatgaaaat ggccgtcgtc
    10321 ggaaacggca acgcttctaa agaacaagtc caatacatgg ttaaaatggt actgggtatc
    10381 actaaagaaa tgagttttga tgaatccgat gccctaggtt tggctatttg tcgtgctcaa
    10441 cgtatcaaaa caccacgcgc ttctgtttca agctggaaag cttatataaa agctcatccg
    10501 gatcgcgtaa aagtaaaata gctttaaata aaaaaaccga tgtagcacat cggtttttac
    10561 gttttatttt aatagtgtca tgcgatgcgt tcgcgaatat ttggatttat tctgcaaact
    10621 tgttgccgtt acgcggtata agtatacgcc cgatgccacc agactatttc gattgttggt
    10681 accgttccat tgcatcgtat gcattcctgc cggaagatcc ttgttaacca gttctttaac
    10741 ctcttgtcca agtaaattat aaatagtcac tacgacatgc gactctgatg ggatttgaaa
    10801 gcgaatcaat gtactcgggt taaacggatt cggatagttg gggaacacat cgaacacttg
    10861 gggtgtttcg attatgattt ttttctcctg atggatagat aaaactccat cataggacac
    10921 atccatcaaa cgatatacat atgttttacc gatttcaacc ttgtcatcgt ttaatatata
    10981 atccgtactg gtatttttag taccctgccc cgttatttct gccactctcg tatattgtaa
    11041 atgagaggtc ttaccctgta gatcgtcctc gttttcaata aaattcgatt cagcgcgttc
    11101 aacaagccag taagcattat tgatttcgct ttcagtacgc catttcaata cagctttgtt
    11161 accattttgt accacatcaa aaaaggccaa ctccaccggc aatgttgcat ctccgccgac
    11221 aacacccgtt tcgacactcg taaacacttt gtatgtaagt gcgctggctg ttgatgcatt
    11281 acgtaatccg gatgtcgaat caatgatcac tgttactgac gtttggttcc caatcgtgac
    11341 tgacgccggt accgttacct ccaaagtatc ttcattgagt gttgatagac ccaaacgcag
    11401 agccgctgcc gcggtaccat tgaccgtaac cgtacttaca gtgggtgtac ccgtagtaaa
    11461 cgtcgcatcg tacggtaaaa gtagcgatat cgtgctcgtc ccggataata gtttccctcg
    11521 taaaccggtt ttaaaagtat aattataacg gccaaattca ttgggcccat cggatgttcc
    11581 ggattgcgtc aacgtcatcg taaaattggt tacggtcgtt gttgccgctt taaccgtata
    11641 attggaactt gtcgtcaaat tgggttgcgg cgtcgtggat acttgcacgg catacgtacc
    11701 tgctactgag ggattgataa taccgcaagc cgttgcaaaa aacaaagtaa cattggtgct
    11761 cgcattaaca ttggtcggtg atgtcactgt taccgtacgc gtaccactat tagtcgcaac
    11821 gaccgaagcg gcaacgctgt tgacagtcac attagaagcc gtgatcgaag ccggtatgac
    11881 cgtattgttt ggaaatacaa cagttattgt actcgaccca ctcgtaagcg caccacccgt
    11941 cccagtagta aaataaatcg agtcccctcc ggccatattg accgtatccg tattgggtgt
    12001 aaaggctaac gaaacaccgg ttaatagcgg agcgttattg atcgtgtatg acgggctggt
    12061 gatagagccg ctttctacgg tagtataagc tgacaacgta tagtttcccg ccgttggatt
    12121 tgtaatcccg ttgatcacaa cgctagccga accggagttt ccgatcgaaa cggaagccgg
    12181 tatagctgct gtcactttta aacccgtgcc ataagccgaa gcgcttaccg tattgatagt
    12241 tactgtcgga gatgcaggga cggtacttcc cgagggaaaa actatcgaaa cagagtccgt
    12301 cgtagaacga aggcggccgt tggtacctgt attgaatgcg attgtataat ttgatgatgc
    12361 cgatggtgta cttgaactca acgtaaccga tggtgtcgtg atcgtagtgg aagtggccgt
    12421 tgtggtatat tgtggtgaag ttgtgggacc ttcaaccacc gtaccaccgg aggtttttgt
    12481 tgtaaccgtc aacgaatatg atgtcgaaac cccggtcgga ttgagaatgc cggcacttga
    12541 acttatagca atagtgacat tagctacatt attgatcgtt acaggattgc ggattctcaa
    12601 cacattattg gaacctaccg ctgaggcagc taattgactg ccattaactg taataagtga
    12661 agatgaaata gtacccggca gatatgtatt ggacggaaat gtaatcagaa tcgagtcgtt
    12721 gatggctaat gcgccggatg agcccgtcgt aaacttgatc gtataagccg ccgtggcgtt
    12781 gatcgtggaa ggtgaaggtg tcacctgtgt aacggccaat gccgaactag cagcaaaaac
    12841 catattggat gttttagtcg aaagcgtatt ttctgcatcg gttgcaacct gtacctgata
    12901 tgaccctgcc gaaccaccat tggtgagtcc tgcggaagcg ctaaatgtaa ccttcgattg
    12961 agtgcttgca ggaaccgatg cgccgctagg catctcgatt tttacttccc cattggctcc
    13021 tgaagtgaca acgcgcacac cggttgaact gacgccgtta atggtaacat tagcggctgt
    13081 aatagacgac ggaactacaa cgcctccgga agtaaatcga attcgaatcg agtcagatcc
    13141 ggcgcttgtt ccacctgcca attttccgaa agctcccgtc gtaaaataaa cttcatagat
    13201 cgcattggtc tgactgacca cattcgacgt tacgaccaca gtatcggccg tgacactggt
    13261 agtattggac gaaacggtat atgccggcga ggtggccggt tgcgtattag tggtcgttac
    13321 gacattgact ttataaaagg tagtggacgg cacggaagga ttgactatac cggcgccgga
    13381 tgcaaatact atagccgcga acgttttatt attagatcct actgtaatat ccgcaaatcg
    13441 tgatgaagta ttggttgcaa ccgacgtaac ggtctgacct gtaccgccgg taaatgtaac
    13501 gttagcggca gctatggacg aaggaacggt tgtgccttcc ggaaaagtca cgcgaacaat
    13561 gtcaccggtg ttaagattac ttacaccggt acagttaatc gtataagccg atgtttgatt
    13621 cacaccgctg tttcctaatg tcacggatga tatcgtgacg gccgtaccgc cgatcgtaaa
    13681 agattgtgaa acgacgtatg atgcttctac ggacgtagca agcgaaatcg tataagaacc
    13741 attagccgga ttggttatgc cgccgataac caatgtaacg gaagcactat tgctaagaga
    13801 aacagtcgaa ggcaaaataa cactcaccgt atcaccgctt cgcgtgggag tagatgcgga
    13861 tgtaccattc actgtccccg ttagcgtgcc gtatccggtt cctgatggaa attttacttt
    13921 aaatacactt gagcccgata acatgcgtcc ataactacca agagtgaatc caagcgtgta
    13981 tgacacattc gtttgagccg ccgtcgtgtt actcaatgca accgatgatg aagataccgt
    14041 cgtcgtcgct gcggcaatgt tatacgaatt agacgcctgg gcattatcaa tcgtagcgcc
    14101 gccactattg accgttttaa cggataattg ataattgccc gatacggccg gattttgaat
    14161 attcgttgat aaagcgatct gaacgctaac agag
//
LOCUS       DAOQNQ010000053        13915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1562_length_13915_cov_6.065440, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000053 DAOQNQ010000000
VERSION     DAOQNQ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13915
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1562_length_13915_cov_6.065440"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2434)
                     /locus_tag="PK754_05290"
     CDS             complement(<1..2434)
                     /locus_tag="PK754_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNB08950.1"
                     /translation="MSLSAICIRRPVLAIVMNIVIVLFGAIAFSYLGVREFPSIDPPI
                     INVRTSYTGANAEVIETQITEPLEKAINGIAGIRTITSSSNQGTSNITVEFTLDVNLE
                     TAANDVRDKVSQAVRQLPQDIDAPPVVTKADANSDAIISMTVESTTKNQLQLSDFAEN
                     VIAERLQTIPGVSSVQIWGQKRYAMRIWMDPAKLSAFGLTPLDIRNALDRENIEAPSG
                     KISGDATELVVRTIGKLITEDDFNNLIISSTGERTVRLKDLGYARLGPENEETILRQS
                     GVPMVGCAIIPQPGSNYIEIADEFYKRFEQLKKDLPKDFSLNIALDNTRFVKRSVAEV
                     QETLLIAIALVVLIVFLFFRDWIVAVRPLIDIPVSLIGAFFIMYTMDFSINILTLLAV
                     VLATGLVVDDGIVVTENIYKKIEEGLSPMEAAFKGSAEIFFAVISTSITLAAVFLPII
                     FLEGFVGRLFREFGIVIAGAVLISAFVSLTLTPMLSSRLFRRQQKHTRFYELTEPFFA
                     GMIQAYGNSLHAFMKRRWLALAVLVVSVLLIFVVGKQLQSELSPLEDRSFIRFQVTAP
                     EGSSFDYTDNFMFNMVQLVNDSVPEKRIFLTVTAPGFAGTGAVNSAFGRLVLIEPDER
                     KRSQQEIAAKLTNYTRKYSEARTFIVQEQTISASGGGARGGLPVQYVLQAPNFERLKE
                     VLPKFMEEVAKEPTFQGFDANLKFNRPELQITIDRDRARSLGVSVLDVGQTLRLALSG
                     QRFGYFVMSGKQYQIIGQVNRRDRDEPLDLKSLYVRNRTGELIQMDNLVKVSEQSGPP
                     QLYHYNRYSSATV"
     gene            complement(2516..3586)
                     /locus_tag="PK754_05295"
     CDS             complement(2516..3586)
                     /locus_tag="PK754_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB08951.1"
                     /translation="MAIKKYLFWGILAGIVIWLTVSRLLKEATSSPGGGNRGGGGNAP
                     MIIKAKIIKPQTLANKIQSTGSVIANEEVELRSEVSGRVVRVYFREGSIINKHDLLIK
                     INDAELTAQLERAESKRKLAESNEVRQRGLFEKNLASQNDYDIALNELNVAKADIELI
                     KAQISKTEIRAPFNGVIGLKYVSEGSYITPTTRIASLQNMDAIKLDFSIPEKYSGAVR
                     VGDDIEYRIEGSDKLYKGKVFAVEPKIELATRTLQLRAISTQIDQRVLPGAFANITLI
                     LRDIPGALMIPTEALMPELKSQRVYVYKSGTAVSQKVETGIRTDVDVQITSGIAPNDT
                     IIITGLMQLRNGLPVRISDLIQ"
     gene            complement(3576..3950)
                     /locus_tag="PK754_05300"
     CDS             complement(3576..3950)
                     /locus_tag="PK754_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNB08952.1"
                     /translation="MADRYKQFTEYRQKMNERILAEDNKVIKRFFGLDTLTYQDGALN
                     IKTKEMLGLVASLVLRCDDCIAYHINKSKEAGVTREEMFEVFSVGLVVGGSIVIPHLR
                     RAVEFLDEVERIETTEPHSHGH"
     gene            complement(4067..6142)
                     /locus_tag="PK754_05305"
     CDS             complement(4067..6142)
                     /locus_tag="PK754_05305"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNB08953.1"
                     /translation="MFYRTVKKALLFVAVPLFCINNDSLLYAQTTSDSLHLSSTDTST
                     IRPDSAMVKPFPYRSVQHFFEKIDTTENIQRGDSSFRYTILPHDSLLHYYHEDFADML
                     MQITGTYLHDQGSYGKPISVTWSGLSNRHILILLDGMPLTEPDWGWTNLNYVSLETID
                     RIEVFRGNASVRYGAQAATGYINIVLRQADADKPLTSLKFRSVFDDFRDVGVFFGRNF
                     GSRVQTYVGGSSRGTPGEQLAIGLSGGTLANFNSTRYSGKNIFFGGKVLIHENIALQI
                     HRQIYTDRYDAYGRDRDPLKDEFDFSTRGAERKDERDDWQARLVVNNKAHMWTTEYQN
                     TDIVRRSTGFTYRLVPRYYGVKNQTYRTRYAGDMGVWSPVVGAAYDRTSFDTLNTRFD
                     TFQMTSLFTENTFRWASWTAMMGMRYDHHSVYDDGVTWTASVAYPIQKKLTLRADVGS
                     TVVFPSLHDEMIHTTSLYSDAFADGYTRSYFFPKDSLSLLPVTRIRTISGTMAHHSSG
                     RGLIVSATIYTNFIKDGTYYTPVHVHTDTERVVPKTKTSGRTWGFDIEASHPFSFTTW
                     TLRQSVSQGDQVFRSGTPWYRASLTGYGEKMVFQNNMRLTGTITASYWNKPTGYTFQD
                     APAMYFVPIASNRSGGFLFFARVSAYVGDLQIFYEAENIFRNRFELMDGYAVTPQQWR
                     FGLVWKLYN"
     gene            complement(6149..7639)
                     /locus_tag="PK754_05310"
     CDS             complement(6149..7639)
                     /locus_tag="PK754_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNB08954.1"
                     /translation="MNLFLLVITVYVLVLIGIGVYKSFQIKSQDDFMVAGRNVSMWFL
                     VGTLVCTWVGSGTLFGSAGLSFRDGFSALWMSAGAWVGIVCVYFLAGRVRKIAEYTVP
                     DILEKRYNATARILGTITIVIAYMSIAGYQFKGGGRLLNIITRTDPHNAATGLDPFVG
                     ALITCGVVVLFTLLAGMVSIIAADLVNGLIITISVIIGVPLLLFGSDIGSWNFVTETL
                     PNDHFTALGTHNAWWVIGIFFPTFFLLMGESSIYQKFFAAKDEATAKKAVIGMVIGVI
                     FVEGMLCLLSIIGSAKHWSNPIFKTADGGIDPSMSETVVLHIARFDLPEVAGALLMAA
                     AVAIILSTANTFLMIPSTNITRDIYQRFINPQADQKTIITMQRVLIVVIALAAFLLAT
                     QFQTILAMAFTAYTMVGAGITPVLLASFLWKRVTPQGGVASMVASMIVTVGITVANAV
                     MDQPLLEADYIIIPAIISSLGCLIFVSLMTKPSPEEKYAPFMSAGQ"
     gene            complement(7655..8446)
                     /locus_tag="PK754_05315"
     CDS             complement(7655..8446)
                     /locus_tag="PK754_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNB08955.1"
                     /translation="MRNQDEEPLNEWDLPEEPESDEEDPEAAHIALTKEMYYTAIIEN
                     IYNEDTEKRREAIDLICHFECDPRLEEPLMKLVRSTDARFVLSGIVGLGNWRHTDGAE
                     VLIEMINHPTPGVLWDMEMLQEALIALGRIGGQEPLAFLKTYAHQRYHDHDGGEDTLG
                     MAAIEAITEIATHGQHGAVEFLIEGCRSLAWNMREACADSFSVVYAGKEQLPKNVYET
                     LVTLTKDENKNVRIAAYMSLDDIVGLDEKNKQILADARHKQVFGK"
     gene            complement(8446..9159)
                     /locus_tag="PK754_05320"
     CDS             complement(8446..9159)
                     /locus_tag="PK754_05320"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNB08956.1"
                     /translation="MDAGSAIRTDTGGMFYSWTWIKWKNFDLMNYNDYILAIIPVFVA
                     IDVIGFMPLYITLTDGIPEPVKSRVLRQSILTAFLVTMTFLALGKAIFAFLGISISDF
                     KIAGGIILFAIAINDLISSSDDRISNEVRDETTEIHINQNPTIGIVPIGIPLIVGPAL
                     LTTMMITIDSYGMGPTIFSVMINLLIVYGALHFSPYVLRITGRSGAKGIAKVFTILLA
                     AIGVMMVRVGITEIINGKV"
     gene            complement(9080..9505)
                     /locus_tag="PK754_05325"
     CDS             complement(9080..9505)
                     /locus_tag="PK754_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB08957.1"
                     /translation="MTKEMWLNLPVKDIKKARAFYSHLGFAFNSERDTEHTACMLVGD
                     QDAGKETIVMLFSEHVFQSFTHHPIADTRHGSQVLFSIDAESREEVNRLAARAEAAGG
                     KVFAKPAENTAWMYGCGFCDPDGHRWNVLFMDMDKMEKF"
     gene            complement(9655..10389)
                     /gene="rsfS"
                     /locus_tag="PK754_05330"
     CDS             complement(9655..10389)
                     /gene="rsfS"
                     /locus_tag="PK754_05330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNB08958.1"
                     /translation="MGSSKKTKKATPKAGKSSKPAKKKAAPKTGKTLKKSGTAVNKTK
                     KSVKTVKKAAKTAVSKKTKTVKKVVTKKTKPVKKDALIAQDEMMDEDVDRLTTEEIDA
                     ELAAYESEKGKPRDPKTGKTLPESEAMQLCRRVTEYMFEKKAEDVVIADLEGLSSAAD
                     FFVICTATSDMHAKAIADNVEDRLAQEGAKLHHKEGHQSLKWVLLDYVDVIVHVFQKD
                     ARSFYDLERLWGDAKFTKVRDTAGRA"
     gene            10511..10594
                     /locus_tag="PK754_05335"
     tRNA            10511..10594
                     /locus_tag="PK754_05335"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10545..10547,aa:Leu,seq:tag)
     gene            10642..11925
                     /gene="tig"
                     /locus_tag="PK754_05340"
     CDS             10642..11925
                     /gene="tig"
                     /locus_tag="PK754_05340"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNB08959.1"
                     /translation="MQASVIHEDNATRKLAVEVPYDAMLPYFEEALAMYREEAQIEGF
                     RKGKAPKDVIQKKFGNMIEAEALEVVVEDFYREALEATKTEAIAIGKIENMDYKKGQP
                     LKFDVTVEIMPKYDVQNYKGLNLKREVHKITDEEVTGAFDRLRESYSTTKETDVIAAG
                     NFATVDIQVLDESGTPLIGKKYEDRRIPLTRQFVGQDFIDGLIGAKTGDMRILDVAKR
                     PPQEGEDIATIEKEKYQVTVRKIEEMQLPALDDEFAKDLGFETFELLKADMHKNLSER
                     WEQESEQKFREALIDEIIKQNEIPAPEPLVDNSLQRIGRMIKERLKGQPVKDELIAER
                     YRPSAIREVKWTLAKRKIIEQENLKVEDADIQAYREKTAKQNKVPLEQINVHFHTETE
                     QRNFEDYLLEEKAAKLIADSAKVEEVPDMLSEEKK"
     gene            12115..12705
                     /locus_tag="PK754_05345"
     CDS             12115..12705
                     /locus_tag="PK754_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNB08960.1"
                     /translation="MSGSVVPYVIEQKGQMERVYDIYSRLLMERIIFLGTPINYEISN
                     VVIAQLLFLAATDPEKDIIMYINSPGGSVTDGLAIYDTMEFIKPEIQTTCTGMAASMG
                     AILLMAGTKGKRYALPHSRIMLHQPLGETQGQSSDIEIYMQEIVKMKDQLNKIIMQRT
                     GQNAERLEKDIDRNFFMSSDEAKAYGIIDEVLVRKK"
     gene            12824..>13915
                     /gene="clpX"
                     /locus_tag="PK754_05350"
     CDS             12824..>13915
                     /gene="clpX"
                     /locus_tag="PK754_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNB08961.1"
                     /translation="MARTVFCSFCGKSAEEVDSIISGPNGVHICNECVRNAFDMIRSH
                     TTRDVSLPLTDITPEKLKLKLDEYIVGQDFAKKSLSVAVYNHYKRINALQDSENDVEL
                     EKNNILLIGPTGTGKTLLAQTLAKFLNVPFTIADATTLTEAGYVGEDVENVLVRLLQA
                     ANYDVQAAERGIIYIDEIDKIARKGGNPSITRDVSGEGVQQALLKILEGTVANVPPKG
                     GRKHPEQNLISINTKNILFICGGAFEGIEKIIQTRVGKKNIGFTASADKKAEQSVSEV
                     LKLVEPDDLLGFGIIPELIGRLPVISPLEELSESALYSILVQPKNALVKQYKKLFALE
                     HVELEFDEKALKEVVKLAVRRKTGARGLRA"
BASE COUNT         3879 a         3449 c         3100 g         3487 t
ORIGIN      
        1 atacggtcgc ggaggaatag cgattgtaat gatacaattg cggcggaccg ctttgctcgc
       61 tgactttgac cagattgtcc atctgtatca gttcgcccgt gcggttgcgg acgtacaatg
      121 atttcaaatc cagcggttcg tcgcgatccc ggcgattgac ttgcccgatg atctgatatt
      181 gttttccgct cattacaaaa taaccaaacc gctggccact cagcgccaaa cgaagcgtct
      241 gtcctacatc taacaccgaa acaccgaggc tacgggcacg atcgcgatca atcgtgatct
      301 gcaattcagg gcgattaaat ttaagattgg catcaaaacc ctgaaacgtc ggttctttag
      361 ccacttcttc cataaatttc ggcaaaactt ctttgaggcg ctcgaaattc ggcgcttgca
      421 gtacatattg caccggcaga ccgccgcgcg caccgccgcc cgaggcagat atcgtttgtt
      481 cctgcacgat aaacgtacgc gcttccgaat acttgcgtgt atagttggtg agtttagccg
      541 caatctcctg ttgtgatcgt ttgcgttcgt caggttcaat caacaccaat cgcccgaatg
      601 ctgaattaac ggcgcccgta cccgcaaatc ccggcgccgt cacggtcaaa aagatgcgtt
      661 tttccggtac agaatcattg accaactgca ccatattgaa cataaaatta tcggtataat
      721 caaaggatga accttccggt gcagtcacct gaaaacgtat gaaactgcga tcttcgagcg
      781 gcgaaagttc ggattgcaat tgttttccca cgacgaaaat taaaagcacc gacacgacca
      841 gaacagccaa cgctaaccag cgacgtttca taaaagcatg caacgaatta ccatacgcct
      901 gtatcatccc tgcaaaaaaa ggctccgtca attcataaaa acgcgtatgc ttttgctggc
      961 gacgaaaaag gcgcgaactc aacatcggcg tgagcgtcag cgatacaaat gcagaaatca
     1021 atacggcacc cgcgatcaca ataccaaatt cgcgaaaaag acgtccgaca aaaccttcta
     1081 aaaaaatgat cggcaaaaaa accgcggcaa gcgtgatgga cgtagaaata acagcgaaga
     1141 aaatttcggc tgaaccttta aacgccgctt ccatcggcga aaggccttct tctatttttt
     1201 tataaatatt ttccgttacg acgataccat catccaccac aagccccgtg gccagtacga
     1261 cggccagcag cgtaaggata ttgatggaga agtccatcgt atacataata aaaaacgccc
     1321 cgatgagtga aaccggaata tcaatcaaag ggcgaaccgc tacgatccaa tcgcggaaaa
     1381 acaaaaacac gatgagtacg acaagcgcaa tcgcgatcaa tagcgtttct tggacttccg
     1441 caacggaacg tttgacaaaa cgcgtattat ccagagcgat attgagcgaa aaatctttgg
     1501 gcagatcttt tttgagttgc tcaaatcgtt tgtaaaactc gtccgcaatc tctatgtaat
     1561 tcgaacccgg ctggggaatg atcgcacatc cgaccatcgg cacgcccgat tggcgcaaaa
     1621 tcgtttcttc attttcaggc ccaagccgcg cgtaaccaag atccttaaga cgcacggtgc
     1681 gttcacccgt cgaagaaatg atgagattat taaaatcatc ttcggtgatt aatttaccga
     1741 tggttcgtac gacgagctct gttgcatcgc cggaaatttt tccgctcggc gcttcgatgt
     1801 tttcccgatc caatgcattg cgtatatcca aaggggtcaa tccgaaagcc gaaagtttag
     1861 ccgggtccat ccagatacgc atcgcataac gtttttgccc ccagatctgt acactactca
     1921 cgccgggaat cgtctgaagt cgctcggcaa tgacgttttc cgcaaaatca ctgagctgca
     1981 attgattttt ggtcgtgctc tctaccgtca tcgaaatgat ggcatccgaa ttggcgtcgg
     2041 ctttggttac taccggcggc gcatcaatat cttgcggaag ttgacgcacg gcttgcgaaa
     2101 ccttatcgcg aacatcatta gctgccgtct caagattcac atccagtgta aattcgaccg
     2161 taatattgct ggtgccttga ttgctggacg acgtgatcgt acggataccc gctataccat
     2221 tgatggcttt ttccaaaggt tcggtgattt gcgtttcgat gacttccgca ttggcaccgg
     2281 tataggacgt acgaacattg atgatcggcg gatcaatgga gggaaattcg cgaaccccaa
     2341 gataactgaa agcgatcgcg ccaaaaagca cgatgacgat attcatcact atcgcaagta
     2401 ccggacgacg aatacaaatc gctgaaagac tcatagacaa gctttctcaa tagaatgatc
     2461 gccgatcgct tcaaaattta aattgatgcg atacgggcat tttagctgta aatagttact
     2521 gaatcagatc gctgatgcga accgggagac cattgcgcaa ctgcataaga cctgtgatga
     2581 tgattgtgtc attcggagcg ataccgcttg taatctgaac atccacatcg gtacgaatcc
     2641 cggtttcaac tttttgagaa acggcggttc cggatttata gacatacaca cgttggcttt
     2701 tcagttcagg catgagcgct tcggtcggta tcatcagcgc gccgggaata tcgcgcagga
     2761 tcaaagtgat atttgcaaaa gcgcccggca aaacgcgctg gtcaatctgt gtcgaaatag
     2821 cacgcagttg cagcgtgcgt gtggccaact cgattttcgg ttcgacggca aacactttgc
     2881 ctttatataa tttatccgaa ccttcaatcc ggtactcaat atcatcgcct acacgtacgg
     2941 cgccggaata tttttctgga atggagaaat ccagtttgat cgcatccata ttttgtaggc
     3001 tggcgatacg tgttgtcggc gtgatatagc tgccttcgct gacatacttc aaaccgatca
     3061 caccgttgaa cggcgcacga atctccgttt tggaaatttg cgctttgatc aattcaatgt
     3121 ccgctttggc tacattcagt tcattgagcg ctatgtcata atcattttga ctggccaagt
     3181 tcttttcaaa aagtccgcgt tgacgcactt cgttggattc ggccaatttg cgtttagact
     3241 ccgcgcgctc cagttgggct gtcagttcgg catcgttgat tttgattaaa agatcatgct
     3301 tattgatgat actgccttca cgaaaataaa cccgcaccac acgcccggaa acttcactgc
     3361 gtaattcgac ttcttcgttg gcaatgacgg acccggtgga ctgaatttta ttagccaaag
     3421 tttgcggctt gatgatttta gctttgatta tcatcggtgc attgccgcca ccgccgcgat
     3481 ttcctccgcc cggtgaagaa gtcgcctcct tcaaaagacg gcttacggtc aaccaaatca
     3541 caatccctgc taatataccc caaaacagat atttcttaat ggccatgcga atgcggctcc
     3601 gtggtttcga tacgttcgac ttcatctaaa aattcaacag cccggcgaag atgcggtatc
     3661 acgatagaac cgcccaccac cagtccgacg ctgaacactt caaacatttc ttcgcgagtc
     3721 acgcccgctt ctttggattt attgatatgg taagcgatac agtcatcgca acgcaataca
     3781 agcgacgcga caaggccgag catttctttg gtttttatat tcaacgctcc gtcctgatac
     3841 gtcaacgtat caagcccgaa aaaccgtttg atgaccttat tatcctccgc cagaatgcgt
     3901 tcattcattt tctgccggta ttcggtaaat tgtttgtaac gatccgccat gtgttttacc
     3961 atcaaataat gtgtgaatga ataaagttaa aaaaccaaat agccgcaagc ataaacgtaa
     4021 aacaattatt aaaattgatt aacccgcgct atcttctctg aaatattcaa ttatatagtt
     4081 tccacaccaa accaaaacgc cattgctgcg gcgttacagc ataaccatcc ataagttcga
     4141 agcgatttct aaaaatattt tccgcttcat aaaaaatctg caggtcgccc acatacgccg
     4201 atacgcgcgc aaagaaaaga aaacctcctg atcggttaga tgctatcggc acaaaataca
     4261 tcgccggcgc atcttgaaac gtgtaccctg tcggcttatt ccagtacgac gccgttattg
     4321 tacccgttag acgcatattg ttttgaaaca ccattttttc gccatagccg gtcagggatg
     4381 cccgatacca cggtgtcccg ctacgaaaca cctgatcacc ctggctgacg gattggcgca
     4441 gtgtccatgt cgtaaaagaa aacggatggg acgcttcaat atcgaatccc catgttcttc
     4501 cgctggtctt ggttttaggt actacccgtt cggtgtcggt atgaacgtga accggtgtat
     4561 aatacgtacc gtccttaata aagttggtat aaatcgtagc ggacacgatc aaacctcgac
     4621 ccgatgaatg atgcgccatg gtgccggaga tcgtacgaat acgcgtcacc ggaagtaaac
     4681 taagtgaatc cttaggaaaa aaataactgc gggtatatcc gtcagcaaat gcatccgaat
     4741 acaacgatgt cgtatgtatc atttcgtcat gcaacgatgg aaaaacaacg gtacttccga
     4801 catcagcgcg aagggttaat ttcttttgta taggatacgc cacagacgct gtccacgtca
     4861 ccccgtcatc atataccgaa tgatgatcat agcgcatacc catcatagca gtccatgatg
     4921 cccatcgaaa tgtattttcc gtaaataacg aagtcatctg aaatgtatca aaacgcgtgt
     4981 tgagcgtatc aaacgatgtt cgatcatacg ctgcgccgac tacgggcgac caaaccccca
     5041 tatcgccggc ataccgggta cggtaggttt gattttttac tccgtagtag cgtggaacta
     5101 aacgatacgt aaatcccgtg gagcgtcgca cgatatccgt gttttgatat tctgtcgtcc
     5161 acatatgcgc tttattattt acgactaaac gtgcttgcca atcgtcgcgc tcgtctttgc
     5221 gctctgcgcc gcgtgttgaa aaatcaaatt catcctttag cggatcacga tcgcgtccat
     5281 aggcatcgta tcggtccgta tagatttggc gatgaatctg caatgcaatg ttctcatgaa
     5341 tcagtacttt accgccgaaa aaaatgtttt ttccggagta ccgggtactg ttaaaattgg
     5401 ccaaggtacc tccgctgaga ccgatagcca gttgttcacc cggcgtgccg cggctggaac
     5461 cgccgacata cgtctgtacg cgcgaaccaa aatttcgccc gaaaaataca cccacatcgc
     5521 ggaaatcatc aaacaccgaa cgaaacttga gtgatgtgag tggcttgtcc gcatccgctt
     5581 gacgcaatac gatattgata tatccggttg cggcttgtgc gccatatcgc accgacgcat
     5641 taccccgaaa cacctcgata cgatcaatgg tttcaaggct tacataatta agattggtcc
     5701 aaccccaatc cggttctgtg agcggcatac cgtcgagcaa tattaatata tgacgattgg
     5761 ataatcccga ccatgttacc gagataggtt taccataact accctgatcg tgtaaataag
     5821 ttcctgttat ttgcatcagc atgtccgcaa aatcttcatg ataataatgc agcagcgaat
     5881 catgcggcaa aatcgtatac cggaatgaag aatctccacg ttgaatgttt tccgtcgtat
     5941 ctattttttc aaaaaaatgt tgaaccgaac gataaggaaa cggtttgacc atcgccgagt
     6001 cgggccgaat cgtagaagta tctgtcgagg ataaatggag actatcggag gtcgtttgtg
     6061 cgtaaagaag ggaatcgtta ttgatacaaa atagaggcac cgctacaaag agcagtgcct
     6121 ttttaactgt cctataaaac atagtgtgtt attgcccggc gctcataaag ggcgcgtatt
     6181 tttcttccgg cgacggtttg gtcatgaggc tgacaaagat caaacaaccc aatgaagaaa
     6241 taattgcagg aataataata taatcagctt caagcagcgg ttgatccatg acggcattgg
     6301 ctaccgtgat accgacggtc acgatcatac tcgcaaccat cgaagcgaca ccgccctggg
     6361 gcgtaacgcg tttccataaa aagctggcaa gcagtaccgg cgtaatgcct gcaccgacca
     6421 tcgtataggc ggtaaacgcc atggctagta tggtctgaaa ctgcgtcgcc aataagaagg
     6481 ccgccagcgc gatgactacg atcagtacac gctgcatggt aatgatcgtt ttctgatcgg
     6541 cttgcggatt gataaaacgc tgataaatgt cgcgtgtaat attggtcgag ggaatcatca
     6601 aaaaagtatt ggccgtggat aaaatgatcg caaccgcggc cgccatcaat aacgcaccgg
     6661 caacctccgg caaatcaaat cgcgctatat gaagcacaac cgtttccgac atcgaaggat
     6721 caattccgcc gtccgccgtt ttgaatatag gattggacca atgtttggcg ctgccgataa
     6781 tagacagaag gcacaacata ccctctacaa aaataacacc gatgaccatg ccgatgaccg
     6841 ctttttttgc cgtcgcttca tccttcgccg caaaaaactt ttgatagata ctgctttcac
     6901 ccatcagcag aaaaaatgtc gggaaaaaga taccgatcac ccaccatgca ttatgcgttc
     6961 ccaatgcggt aaaatgatcg ttgggcagcg tttcagttac aaaattccaa ctgccgatat
     7021 cactgccaaa caacagcaaa ggaacaccga tgataaccga aatcgtgatg atcaatccat
     7081 tgacaagatc agccgcgatg atagacacca taccggccaa taatgtaaac agtacgacga
     7141 caccgcacgt gatcaaggcg ccgacaaacg gatcaaggcc cgttgccgca ttatgcgggt
     7201 ccgtacgggt tatgatgttg agcaaacgtc cgccgccttt aaattgatat ccagcgatgg
     7261 acatataagc aatgacgatg gtgatcgttc cgaggatacg cgccgtcgca ttgtaccgct
     7321 tttccaaaat gtcaggtacg gtatattccg cgattttgcg aacgcgaccc gcaaggaaat
     7381 atacgcacac gatccctacc catgcgccgg ccgacatcca taatgccgaa aaaccgtcac
     7441 gaaacgacaa cccggcgctt ccaaaaagcg tccccgatcc gacccatgtg caaacgagcg
     7501 taccgaccaa aaaccacatg gaaacattgc gaccggctac catgaaatcg tcttggcttt
     7561 tgatttgaaa acttttgtaa acaccgatac cgatgaggac cagtacgtac accgtaatga
     7621 caagaagaaa aagattcata gcgcctcggt ttgattattt tccaaaaacc tgtttatgcc
     7681 gtgcgtcggc gagtatctgt ttgttttttt cgtcaagccc tacaatgtcg tctaaactca
     7741 tataggccgc aatacgaaca tttttatttt catccttagt gagagtgact aatgtctcgt
     7801 acacattttt aggcaattgt tctttaccgg catacactac gctgaagctg tcggcgcagg
     7861 cttcacgcat attccatgca agactgcggc acccttcgat cagaaattcg accgcgccgt
     7921 gctggccgtg cgttgcgatt tcagtgatgg cttcgatagc cgccataccc aaagtatctt
     7981 cgccgccgtc gtgatcatgg tatcgctgat gggcatacgt tttcaaaaac gcaagcggct
     8041 cttgtccgcc gatgcgcccc agtgcgatca gcgcttcctg cagcatctcc atatcccaaa
     8101 gtacgcccgg tgtcggatgg tttatcattt cgatcagcac ttcagcgccg tcagtatggc
     8161 gccagttacc aagaccgaca atacccgaca gcacaaatcg cgcatccgtc gaacgaacca
     8221 acttcatcaa cggctcttct aatcgcgggt cgcattcaaa atgacagatc aggtcgatag
     8281 cttcccggcg cttttcggta tcttcattgt agatattctc aatgatcgcg gtatagtaca
     8341 tctctttggt cagcgcgata tgcgcggctt ccgggtcttc ctcatccgac tccggctctt
     8401 ccggcaaatc ccattcattg agcggttctt cgtcctgatt gcgcattata cttttccgtt
     8461 aataatttcc gtgatcccga cacgcaccat catcacaccg atcgcggcta ataagatggt
     8521 aaatacttta gcgatgcctt tggcgccgct ccgccccgta atacgtagaa catacggtga
     8581 aaaatgcagc gcgccgtaaa cgatgagcag attgatcatc acactaaaaa tcgtcggtcc
     8641 cataccgtag ctgtcgatcg tgatcatcat ggttgtgagt aaagccgggc ctacgatcag
     8701 cggtattccg atgggcacga tgccgatcgt cggattctga ttgatatgta tttcggttgt
     8761 ttcatcgcgg acttcgttgc tgatgcgatc gtccgatgag gaaatgagat cattgatggc
     8821 aattgcaaaa agaataatac cgccggcgat tttgaagtcg ctgatcgaaa tgccgagaaa
     8881 cgcaaatatc gccttgccca gcgccaaaaa agtcatcgta accaaaaaag ccgtcagaat
     8941 agattgacgt agtacgcgcg atttgacggg ctccggtatg ccgtcagtca acgtgatata
     9001 aagcggcata aaaccgatca catcaatagc gacaaacacc ggtatgatgg caaggatata
     9061 gtcattataa ttcataaggt caaaattttt ccattttatc catgtccatg aataaaacat
     9121 tccaccggtg tccgtccgga tcgcagaacc cgcatccata catccacgct gtattttctg
     9181 ccggtttggc aaatactttt ccgccggccg cttccgctct tgccgcaagg cgattcactt
     9241 cctcgcgact ctccgcatcg atcgaaaaaa gaacttgcga accgtggcgc gtatccgcaa
     9301 tcggatgatg ggtaaagctt tggaagacat gctccgaaaa caacatgact atcgtttctt
     9361 tgccggcatc ttgatcgccg accagcatgc atgccgtatg ttcggtatcg cgttctgaat
     9421 tgaacgcaaa accgagatga ctataaaatg ccctggcttt ttttatgtct ttgacaggca
     9481 ggttgagcca catttctttt gtcataacct gagcatccga tacaagtgat atacgcagta
     9541 ctcgctgtat aaatctacga aacgaaaaaa tatatgcagt tcggcgggaa aaatccgtct
     9601 aaataaaaag aacttacaaa cgatgtatac gtaccgaaga caatcgaaaa aacattatgc
     9661 gcgcccggca gtgtcccgca ctttggtaaa tttggcgtcg ccccatagac gttcgagatc
     9721 ataaaacgaa cgcgcatctt tttggaaaac atggacgata acgtccacat aatccaaaag
     9781 aacccatttc agcgattggt gaccttcttt gtgatggagt ttagcgcctt cctgagcgag
     9841 gcgatcttcc acattatccg caatggcttt ggcatgcatg tcggatgtcg ccgtacatat
     9901 cacaaaaaaa tcggcggcgc tggacaatcc ttctaaatcg gcgatgacga cgtcttcagc
     9961 ctttttctca aacatgtatt ccgttacacg gcgacacagc tgcatggcct cggactccgg
    10021 taaggttttt ccggttttag ggtcacgcgg tttgcctttt tcactttcat aagccgccaa
    10081 ttccgcatca atttcttctg tcgtcaaccg gtccacatcc tcatccatca tttcgtcctg
    10141 tgcgatgagc gcatcttttt tgacgggttt ggtttttttg gttactactt ttttaaccgt
    10201 tttggttttt ttggaaacag ctgttttcgc cgctttttta acggttttga cggatttttt
    10261 ggttttattg accgctgtgc cggatttttt taaagttttg cccgtcttgg gcgctgcttt
    10321 tttcttagcc ggtttgcttg attttccggc tttaggtgtc gctttttttg tctttttgga
    10381 tgatcccaag tttttgattc tccgattttt acaatgcggt caatataggg atgccgacac
    10441 tttaaaatca aataaaaact tgctattgca gggctcgttg gctatattgc gccgcatttg
    10501 gcctatatat gcgggattgg cggaattggc agacgcgcca gacttaggat ctggtggttt
    10561 taccgtgtgg gttcaagtcc ctcatcccgc accatttttt gaaaacatta aaataaatat
    10621 ttataacaca ggagaattcc tttgcaagcg agtgtgattc acgaagataa tgctacccga
    10681 aaattagcgg tagaagtgcc ctacgatgcg atgttgccat actttgaaga ggctttggcg
    10741 atgtaccgtg aagaagccca gatcgaaggt tttcgtaaag gcaaagcccc caaagatgtg
    10801 attcagaaaa aatttggcaa tatgatcgaa gcggaagcgc tggaagtggt cgtggaagat
    10861 ttttaccgcg aagcgctcga agcgactaag accgaagcca tcgcgatcgg caaaatcgaa
    10921 aacatggact acaaaaaagg tcaaccgctg aagtttgacg tgaccgtcga gatcatgccc
    10981 aagtacgacg tccaaaatta caaaggcctc aacttaaaac gcgaagtaca caaaataaca
    11041 gacgaagaag tgaccggcgc ttttgatcgt ttgcgcgaaa gctattccac gaccaaagaa
    11101 accgatgtga ttgctgcggg taacttcgca acggtggata ttcaggtgct ggatgaaagc
    11161 ggtacgccgc tcatcggcaa aaaatatgaa gatcgtcgca ttccgctcac tcgccaattc
    11221 gtgggacagg attttattga cggccttatc ggcgccaaaa caggcgacat gcgcatcctt
    11281 gatgtagcca aacgtcctcc gcaggaaggc gaagacatcg caacgatcga aaaagaaaaa
    11341 tatcaggtga ccgttcgcaa aatcgaagaa atgcaactgc cggctctgga tgacgaattc
    11401 gccaaagacc tcggatttga gacattcgaa ttactcaaag ccgatatgca caaaaatctc
    11461 agcgaacgct gggagcaaga gtccgaacaa aaatttcgtg aagctttgat tgacgaaatt
    11521 atcaaacaaa acgaaatccc tgctccggaa ccgctggtag ataattcgct tcagcgcatc
    11581 ggacgcatga tcaaagaacg cctcaaaggc cagcccgtca aagacgaact catcgccgaa
    11641 cgttatcgcc caagcgccat ccgcgaagta aaatggacat tggctaaacg caaaatcatc
    11701 gaacaggaaa acctgaaggt tgaagatgcc gacatccagg cctatcgcga aaaaacagcc
    11761 aaacaaaata aagtaccttt ggaacagatc aacgttcact tccatacgga aacggaacaa
    11821 cgcaacttcg aggattattt gcttgaagaa aaagcagcca aactgattgc cgactcagct
    11881 aaagtcgaag aagtacccga catgctctcg gaagagaaaa aataagctga tattgtacac
    11941 gacatagcta atatctgtat tttaaatttg taaaaactct gactttgctt tttgttcaga
    12001 gtttttgcac tttaaacctt gaatttgatg accgaaacca ggacgtctat gaatccatac
    12061 gaatcatttg tcaaaagtga atttaaccgc ctcagcgacg acagcttgcg atccatctcc
    12121 ggttccgtcg tgccttatgt gatcgaacaa aaaggtcaga tggagcgcgt ctatgatatt
    12181 tattcgcgcc tgctgatgga gcgtattata tttctcggta cgccgattaa ttatgagatt
    12241 tccaacgtcg tcattgcgca gctactcttt cttgcggcga ccgatccgga aaaagacatc
    12301 atcatgtaca tcaatagccc cggcggcagc gtgaccgacg gacttgcgat ttacgatacg
    12361 atggagttta tcaaaccgga aattcagacc acctgtaccg gcatggcggc cagtatgggc
    12421 gctatcctcc tgatggccgg caccaaagga aaacgttatg ccttaccgca ttcgcggatc
    12481 atgctgcatc agccgctcgg agaaacgcag ggacaatctt ccgatatcga aatctatatg
    12541 caggaaatcg tgaagatgaa agatcaattg aacaaaatca tcatgcagcg caccggccaa
    12601 aacgcggaac gccttgaaaa agatattgat cgcaatttct ttatgtcttc cgacgaagcc
    12661 aaagcatacg gcatcataga cgaagtgctt gtacgcaaaa aataattgcg tgtatataac
    12721 attatccggt ccggcatcct ctccgccggg atcttatcgg tttatattgt tgcaaacgcg
    12781 aaccctattt cttattctaa agccataacc tgaaagttag tccatggcac gtactgtatt
    12841 ttgctccttt tgcggtaaat cagccgaaga agtagacagc attatttcgg gacctaacgg
    12901 cgtccacatc tgcaatgaat gcgtacgtaa tgcgttcgat atgattcgct cgcatacgac
    12961 acgcgacgtt tcattaccgc tgaccgacat cacgcccgaa aaactgaaac tgaagttaga
    13021 tgaatacatt gtcggccagg attttgcaaa aaaatcgtta tccgttgctg tctataatca
    13081 ctacaaacga atcaatgcgc tccaggattc tgaaaacgat gttgaactcg aaaaaaacaa
    13141 cattctcctg atcggaccga caggcaccgg taagacctta cttgcgcaaa cgttagccaa
    13201 atttttgaac gtaccgttta cgatagccga tgccacgacg ttgactgaag ccggctatgt
    13261 cggcgaagac gttgaaaatg tgctcgtgcg tctcctgcag gctgccaact atgacgtcca
    13321 ggcggcagaa cgcggcatta tctatattga cgaaattgat aaaattgcac gcaaaggcgg
    13381 caacccttcg atcacacgcg acgtttccgg tgaaggcgtt cagcaggctc ttctgaaaat
    13441 cctggaaggc actgtggcca atgttcctcc caaaggcggt cgcaagcatc ctgaacaaaa
    13501 cctcatcagt atcaatacca aaaatattct tttcatctgc ggcggcgctt tcgaaggaat
    13561 cgaaaaaatt attcaaacgc gtgtcggcaa aaagaatatc ggatttacgg cttcagcgga
    13621 caaaaaagcc gaacagtccg tgagcgaagt actcaaattg gtagaaccgg acgatctgct
    13681 gggattcggc atcattcccg aattgatagg gcgtttacct gtaatcagtc cgctcgaaga
    13741 actgtccgaa tcggcgctct attctattct tgttcaaccc aagaatgcgt tggtcaaaca
    13801 gtataaaaaa ctttttgcac tggaacacgt cgaactggag tttgatgaaa aagcattgaa
    13861 ggaagtcgtc aaacttgcgg tcagacgaaa gaccggcgcg cgcgggcttc gtgct
//
LOCUS       DAOQNQ010000054        13830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1576_length_13830_cov_5.823811, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000054 DAOQNQ010000000
VERSION     DAOQNQ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13830
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1576_length_13830_cov_5.823811"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PK754_05355"
     CDS             <1..672
                     /locus_tag="PK754_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB08962.1"
                     /translation="TSLRASWSRNFRAPTLNERYWKDTDASGNPDIKPERSTTWDLGG
                     RFGGSQAKHSFEIEYTWFVMRAHNQIAWNLAGIIHPENIRKSSSRGWEIAMKYEKEQM
                     WETKLELSRAKAVDTSDPDNVRRIPYNPLYTTSLSNYAMFKDFTIGHTTHYQSQRYTD
                     AVNNSVIPSYWLTDAIVSWHKQIISYDVQLSVIVKNIFDKAYESVAQYPGLAREYVVG
                     TKITF"
     gene            692..1732
                     /locus_tag="PK754_05360"
     CDS             692..1732
                     /locus_tag="PK754_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08963.1"
                     /translation="MRYIGIVLSFTMLLSCEERISDPKSGTITEGIFILNEGSFGAGN
                     GDVSLYRPDSADVINNLYLSANGKSAGDIVQDMLILDTMAVISINNSNKICFVRLKDF
                     TLLSTIGTLQPRMFVKGDDDKVYLTAWDGKLKTLDLAQKRVTDSIAIGTAPESLFLDG
                     NDLYIANYGYGNDNTVKIYNTVSKSITKTIRVGYGPRAFSKSSGDNKLFIACEGNQYA
                     EIPVPGGIYVVDLNTETMVDSLVSLPSSTGAKSFYPGRIAVNENMGYFISGFYGDIQK
                     FNISNWTIIDSIKGSFYNVVLNPDVDSDVYATDASLVPGKFEIFDQDGQVKQSFTVGD
                     FPSGMVFYNKNK"
     gene            1830..2387
                     /locus_tag="PK754_05365"
     CDS             1830..2387
                     /locus_tag="PK754_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08964.1"
                     /translation="MTMRTSNMLRFVPTLVTVLLIVGYRLMPHPWNFVPVTAMAIFGG
                     IYLNRSLAYGLPLASMIVADLFLGMDWTSPVVYASILVSSWIGTRVRNVYENKKQFAV
                     AVGGGTLASSVLFYLTTNFAAWLVIPEYTKDISGLIQSYIMAIPFFKNALAGDMFFIV
                     VFVGVYEAIHFIVTKLSRQSAPIHG"
     gene            2467..3426
                     /locus_tag="PK754_05370"
     CDS             2467..3426
                     /locus_tag="PK754_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08965.1"
                     /translation="MGCNESDDSFSTIPSGLSSVWIINEGNYGTNNASITLLDTLFED
                     TTFSNATGSYLNGSAVHSAVFTDSTTWLMMTGSDKISILHRRTLKAMRTITGIKSPRN
                     GVLYQNNVYVTSYFDSLVIAYNAADYTETNRYKLSHRPDEITALNGLLIVSNSRSPDG
                     VLSKDSVISLIDITMSDVNAYVIGSPVVSVCANNPAGKVYIAYTGKSSSAGKIVEWDP
                     LTKVITSEVVSGINPTFVRMHDSVLVYNTRSNSAIIRYDMKTDIKITLAGSYFVGAPL
                     GEDILAADAQDFLRNGYLYWIRKDGTIRKRYPVGITPSSILQP"
     gene            3432..3983
                     /locus_tag="PK754_05375"
     CDS             3432..3983
                     /locus_tag="PK754_05375"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNB08966.1"
                     /translation="MKIYTKTGDDGTTGLFGAGRVQKDATRIESYGTVDELNSVMGLV
                     QCHSEDDELDMVYAGIQNELFILGADLATPMDVKSEYIIRIKAENTLQIEKRIDSFDV
                     RLPELTNFILPGGTQASSYLHLARNVCRRAERLTVKLSKEEVINEHALTYLNRLSDLL
                     FVLARWENAKAGIADTMWKKPVD"
     gene            3983..4171
                     /locus_tag="PK754_05380"
     CDS             3983..4171
                     /locus_tag="PK754_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNB08967.1"
                     /translation="MITRCICFDKEFEELKKVAAAYRCKTLEELQERVLVGVRCKLCH
                     PYIRRMLATGQTRFEVNE"
     gene            complement(4178..6226)
                     /gene="feoB"
                     /locus_tag="PK754_05385"
     CDS             complement(4178..6226)
                     /gene="feoB"
                     /locus_tag="PK754_05385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNB08968.1"
                     /translation="MTDSVLPSDCHTPSSNSVSGTSSNASFRITLIGQPNSGKTTLFN
                     ILTGSHFKTSNYPGTTVEFLVGRLHERFKCDAQVIDSPGINSLTPQSLDERVSVDSLF
                     LDPNYRHPDLVLVTADATQLSRQLYLVKQLIDAGFPVIVALTMNDLLQKRGFSIDTTL
                     LSERLGTPVIKTYGHTENGVAELAEALRGFFAVPQKYILPPIPKLQSESTVMELYRIT
                     ESIEKEVIRPFSVKPDIHAINHAVFNTRTIEPDETSLKLDRIFLHPIWGMFFFVFFMT
                     LIFTSVFWLAQPMMDVISEGFDVLANLSKSWLPSSHWLGNLIADGLIAGMGTVMTFVP
                     QIVILFLLMGMLEDSGYLSRGAMLIDKPLSKIGLNGRSFVPMLSGFACAIPATMAART
                     IPNQRERLLTIFIIPLMTCSARLPVYALLLGFLMPSDRPWLSGLILSGIYFVSLINGA
                     LVAGIVSRFIKEKTPSSFMLELPAYRKPIIRFILRNTFQRSVSYIQNAGTTIILCSII
                     IWGLTYFPNANPEITDEAKASGNVEQWIAGERTSSSYAAMVGRWIEPALMPLGWDWRV
                     GVGMISAFAAREVFVSATALTFHIAEDDEDNMQQSLINSMRTARHEQTGKPVFTVSSV
                     VAIVIFFMFALQCTSTLAVVRKETGSWKMPLIQQVAFTTIGYGLGWLAVVLLNAVGIA
                     "
     gene            complement(6223..6468)
                     /locus_tag="PK754_05390"
     CDS             complement(6223..6468)
                     /locus_tag="PK754_05390"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNB08969.1"
                     /translation="MTSNKLKTVAQLGCGECAVICKISGEHLENDSTCIRLMEMGFTP
                     GQEVKVIAKSPFRCPMAVSVRGTVIALRKNEAECIHI"
     gene            6681..7034
                     /gene="erpA"
                     /locus_tag="PK754_05395"
     CDS             6681..7034
                     /gene="erpA"
                     /locus_tag="PK754_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HNB08970.1"
                     /translation="MTETMEAVNDVVITPRAIEEIGSIMREQSIPENYALRMGVQGGG
                     CSGFQYSLAFDETISENDTILEFGAVKVAVDYKSMLYLKGVTLDFQDGLNGRGFVFNN
                     PNAKKTCGCGSSFSA"
     gene            7148..7900
                     /locus_tag="PK754_05400"
     CDS             7148..7900
                     /locus_tag="PK754_05400"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNB08971.1"
                     /translation="MNQQTKQYEGKMKRFHCIKRVVVFSFIMGLLGLLSCDDDSAKDT
                     DDGTANNEPGLVAHYPLDGNADDVGNSHSDGVVSGAVSANDRHDQVGKAMYFNGNSDF
                     ISVNDTSNFQFDDSLTVTAWVRTDSLKSQIVVRKGGGLAVPVFELYLAGNGDRGFRIG
                     TNFTYKSGGYPLQTWMHLAGTYDGESMVFYINGSPVDTLQKSNALTSDKNLLLIGTRT
                     GLNADTFAGRLDDIRIYSRSLSLSEIAAMAAE"
     gene            7957..8484
                     /locus_tag="PK754_05405"
     CDS             7957..8484
                     /locus_tag="PK754_05405"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNB08972.1"
                     /translation="MREIRWIAMVSLFFLTGSVVWGQTENSEPPDDAALKHAVENKIQ
                     GIVWYGARDYVNVEVSEGTVILNGWVSGYWKTDKIQKAVEQVPGIKEITNEITVSNGS
                     DELAGRALSAIYRDAMFRKYALAADLPVHVIVQNNSVILAGKVMVESERRRAEFLVDA
                     HTAAAAIDNRLVIID"
     gene            8570..9457
                     /locus_tag="PK754_05410"
     CDS             8570..9457
                     /locus_tag="PK754_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08973.1"
                     /translation="MRFFILILMIPIVNFAVSQTQVDIPEKAEAAYKASDWATSVKLY
                     LQLCKSQPGESKFWNRLATSYYQLGAYKNASETYRQYLSLTQDPVGMYNAACSFALSG
                     EKDTAWYWLEKATQSGWFTSLQVRHDNDLKSLHSRKFDDLVISLDRAARPCEYDSLFR
                     QFDFWIGEWDVFNLAGQKAGTNTIQKLTDGCLLFENWTGSLGGSGKSINFYDKQKKLW
                     RQIWIASNGNVSDFYGQYSEGVMDFYGESVSANGSIGKNRLRFFKIDSDTVRQFAERS
                     DDNGKSWQVTYDFRYVRKK"
     gene            9494..12631
                     /locus_tag="PK754_05415"
     CDS             9494..12631
                     /locus_tag="PK754_05415"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB08974.1"
                     /translation="MKYLILFLLPAFLCAQNNRIRLESLPLDDGLSYQSVYALYQDSR
                     GYLWIGTISGLIRYDGRNNTVFRHEPENPHSLSHDDVVAIYEDEFKKIWIGTYGGGLN
                     RYNPETGVFTRYLNDSTDTKSISDNVILSITGDKNGRLWIGTRTGGINVLNITLPDSG
                     FTRFTRDPKKPRRTAPQAPQKLYTSSDGRIWIGAFGRGLVTYDQSENGFRYFPLTRNS
                     GDTVRFYNAYDIVEINNLLWISTDVGLKTFDLKVDQWIESTFPTKATCDSLSAFRLAV
                     DRINNVLWVGSSGKGLFKFDFNSNTISQNKNHWSYAPYALKTDMVTALLVDQSSMLWV
                     ATANGALQKNAPDFYKFDRLTDLFDHISGEMYVNAILPDSSSVWIGHHGGLVRINKKS
                     IETWEDIFPASHQSIRSVTALWKDSDGALWVGTTTAGLIRIKNRIKTTFLISPKENTS
                     YSFPISSIIRDKKGQLWVGTIDGGLNLFDEIDKSFIRYDPANSIMKSASVSCLYESSD
                     STMWVGTYGGLLQISQEGPWKYFTHDLKNPMSISHNYVYSVFCDQNGRTWVGTNNGLC
                     YKEKNSDDFKVLNQKNGLPSDVIVGILGSSGGKIWVSTHNGISSFDPNQPTSFRNYDK
                     YDGLPGNMFNIGAAIQSRDGTLWFGSIQGAVCFKSDQLRDNLFEPQVQIVQAKKFNQI
                     ISIDSDLIRLNYDENYLSISFSAMDFFAPNKNQYQYRLEPLEKAWHYAGNKGEAQYTD
                     LAPGSYTLTVRATNRDGIWSSQIGAVQVIIRPPFWKTRSFLFLVAMTAFLLIWFVHRS
                     MTKRAVDKSLEMERIRLTERERMREQISKDFHDELGHKLTKITLFSELSQRALHDNKT
                     DSEKFLRKVADTSTSLSVTTRDFIWALDPGKDSLYDVIVNLRDFGFDLVQDTEIVFEV
                     GGLTEQLDRIRLTMEWRRHLTLIFKEAMNNALKHAKAKHIRLRVIIEGIHFAIHLTDD
                     GTGFSQESTRSTRNGLKNMQERAQKLYGLLSIESSESAGTHISFTGLLPTIDAEARNS
                     QEKRDWITTIKGWFV"
     gene            12628..13245
                     /locus_tag="PK754_05420"
     CDS             12628..13245
                     /locus_tag="PK754_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB08975.1"
                     /translation="MIRLMIVEDDPDIREGLELIAIQTRGVKLVGAYNTAETAIEATE
                     KDKPDVLLLDIELPGINGIEAIPLFKKRNENTDILILTVHQDDQTVFDALCAGASGYL
                     IKTAHPNKIMDAVKEINSGGVPMSASIARLVIQSFQKKQNPVLTTRETDVLRFLCKGH
                     SYKMIADSMHVSWETVHSHIKSIYRKLNVNSKSEAVITALKDRII"
     gene            13386..>13830
                     /locus_tag="PK754_05425"
     CDS             13386..>13830
                     /locus_tag="PK754_05425"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNB08976.1"
                     /translation="MRKILPLFWIILVMVWPDRSLLGQMRFVPITDTSNPINTDPLGP
                     AVNNYNGSAWIDFDGDGDLDLYAHIGRLYRNDGGNFVAVTNSPGSGLSNAAVGKGISF
                     GDVDNDGDLDAFVTSTRGSGLYLNNGGTYTRIATGSIGQANAGWAG"
BASE COUNT         4091 a         2624 c         3156 g         3959 t
ORIGIN      
        1 acgtccttac gcgcatcatg gtctcgtaat tttagagcgc ctacgctcaa tgaacgatat
       61 tggaaagata cggacgcatc aggcaatccg gatatcaaac ctgaacgatc aacaacatgg
      121 gatttgggcg ggcgcttcgg tggttctcaa gcaaagcata gctttgaaat cgaatatact
      181 tggtttgtaa tgagagcgca caatcagatc gcgtggaatt tggccggaat aattcatccg
      241 gaaaatattc gcaaaagttc atcacggggt tgggaaatag cgatgaagta cgaaaaagaa
      301 caaatgtggg aaaccaaact tgaactttca cgtgcgaagg ccgttgatac cagcgatccc
      361 gataatgtac gacggattcc gtacaatcct ttatacacaa cttccttatc aaattacgct
      421 atgttcaagg attttacaat aggacataca acgcattatc agagtcaacg ttatacggat
      481 gctgtcaata acagtgtaat accgagttat tggcttacgg atgcgatagt gtcttggcac
      541 aaacaaataa ttagttatga tgtacaactt agcgtaatcg taaaaaatat ttttgataaa
      601 gcttacgaat ccgttgcgca atatcccggg cttgcgcgcg agtatgtcgt gggaactaaa
      661 ataacgttct aacacattgg agaaaaaaag catgcgctac atagggattg ttttaagttt
      721 cacgatgcta ttatcgtgcg aagaaagaat ttctgaccct aagtccggaa ccattacaga
      781 gggtatattt attttaaacg agggctcatt tggcgctgga aatggtgacg tttctcttta
      841 tcgccctgat agtgcggatg tgatcaataa tctttacttg agtgccaatg gcaaatctgc
      901 cggcgatatt gtacaagata tgctgattct tgacacgatg gctgttattt ctattaataa
      961 ttcgaataaa atatgtttcg tgcggttgaa agatttcacg cttttatcaa ctataggtac
     1021 gctacagccg cgtatgtttg tcaaaggtga cgatgacaaa gtttatctaa cggcatggga
     1081 cggaaaactc aaaaccctgg acttggcaca aaaaagagtg acggattcga ttgcgatagg
     1141 cacagcgccg gaatctttgt ttctcgacgg taatgatctt tatatcgcta attacggtta
     1201 cggcaatgat aatacggtga aaatctacaa tacagtgtca aaatcgatta ccaaaaccat
     1261 tcgtgtcggt tatggtccgc gagcatttag caagtcatcc ggggataata aactttttat
     1321 cgcatgcgaa ggaaatcaat atgcagaaat accggtgcct ggcggaattt acgttgttga
     1381 tttaaatacc gaaacgatgg tggattcgtt ggtgagtttg cctagctcta caggtgcgaa
     1441 atctttttat ccggggcgta tcgctgtaaa tgagaacatg ggttacttta taagtgggtt
     1501 ctatggcgac atacaaaaat ttaatatcag caattggacc attatagata gcatcaaagg
     1561 ttccttttac aacgttgttt taaatcccga cgtggattcg gacgtatatg caaccgacgc
     1621 gtcgttagta ccgggaaaat ttgaaatatt tgatcaagac ggtcaagtga agcaaagctt
     1681 taccgttggt gattttccgt ccgggatggt attttacaac aagaataagt gattgcaaaa
     1741 ttatccaagg caaactatat tgacgcgtct gcagtgaata gaaatttatc attcaatcat
     1801 atataacgta taatcaaaag gtaatcatta tgacaatgcg cacgtcaaac atgctgaggt
     1861 ttgttcctac actggtgacg gttttattaa ttgtagggta tcgccttatg ccgcatcctt
     1921 ggaactttgt tccggtaact gcgatggcga tttttggtgg gatctatttg aatcgctcgt
     1981 tggcgtacgg cttaccgtta gcgtcaatga tcgtagccga tctttttctc ggaatggatt
     2041 ggacttcacc ggttgtttat gcgtcgatat tagtcagctc atggattgga acccgagttc
     2101 gcaatgtcta tgaaaacaag aaacagtttg ccgtggccgt aggtggaggt accttagcct
     2161 cgtcggtact tttttatctg acaaccaatt ttgccgcgtg gcttgtaatt ccggaataca
     2221 ccaaagatat atccggcttg atacaaagct atattatggc gattcctttt ttcaaaaatg
     2281 cattggccgg ggatatgttt tttatcgtgg tgtttgtcgg agtatatgaa gcaattcatt
     2341 ttatcgttac aaagctcagt cgccaatcag cgcctattca cggataataa tttgagcgct
     2401 ttattttttt acacctcacg gttgaaattg atgttataca ttttcgccgt ggggattttt
     2461 ttttctatgg gttgtaatga gagcgatgat tccttttcga ccataccgag cggtttatcg
     2521 agtgtatgga ttatcaatga aggtaattat gggactaaca atgcttccat cacattgctt
     2581 gatacgctgt ttgaagatac aacgttttca aacgccacag gttcttatct gaacggaagc
     2641 gccgttcaca gcgcggtttt tactgattca acaacttggc tgatgatgac gggctccgat
     2701 aaaatcagta tcctgcaccg gagaacactt aaagccatgc gcacgattac tggcatcaaa
     2761 agcccgcgta atggcgttct gtaccagaat aatgtgtatg tgacctcgta ttttgacagt
     2821 ttggttatag cgtataatgc cgctgattat acggaaacga atcgttataa actcagccat
     2881 agaccggatg aaataacagc actcaacgga ttgttgatcg tatccaatag ccgctcgcct
     2941 gatggagtat tatccaaaga tagtgtgatc agtttgattg acataacgat gtcggacgtc
     3001 aatgcgtatg ttatcggttc accggtcgtt tccgtatgtg ctaataatcc tgccggtaag
     3061 gtttatatcg cttataccgg taaaagttct agtgccggca aaatagtaga atgggacccc
     3121 ttgactaaag taattacctc tgaagtagtc tccggtatca accctacttt tgttcgtatg
     3181 catgattctg ttttggttta taacacgcgg agcaatagtg ccattatacg atacgatatg
     3241 aaaaccgata tcaaaattac tttagcgggt agctatttcg ttggagcgcc attaggtgaa
     3301 gacatattag cagcagatgc gcaagatttt ttgcggaatg gttacctata ttggattcgt
     3361 aaagacggaa ctattcgcaa acgatatccg gtaggtataa caccaagttc aattttacaa
     3421 ccataaaatc tatgaaaatc tacacgaaaa cgggggacga cggcacgacc ggtttatttg
     3481 gtgccggacg cgttcaaaaa gatgctactc gaatcgaatc gtatggtacc gttgacgaac
     3541 tgaacagcgt catgggttta gtacaatgcc atagcgagga tgacgaattg gatatggtat
     3601 atgcggggat acaaaacgaa ttgtttattc tgggagcaga tttggcaacc ccgatggacg
     3661 ttaaaagtga atatattatt aggataaaag ccgaaaatac cctgcaaatc gaaaaacgta
     3721 ttgatagttt tgacgtacgg ttaccggagc tgacgaattt tattctaccc ggaggcacac
     3781 aggcttcgtc atacctccat ttggcgcgaa atgtttgccg cagggccgaa aggttgacag
     3841 taaaattgtc taaggaggag gtgatcaatg aacatgcatt gacgtacctt aatcggcttt
     3901 ccgacctttt atttgttttg gcacgctggg aaaatgcgaa ggcaggcatt gcggatacca
     3961 tgtggaaaaa gccggttgac taatgatcac acgttgcatt tgttttgata aagaatttga
     4021 agagctcaaa aaagtagctg cagcataccg ctgtaagaca ttggaagaat tacaagagcg
     4081 ggttttagtc ggtgtacgtt gtaaattatg tcatccctat attcgacgaa tgctggctac
     4141 cggtcagaca cgatttgaag tcaatgagta atcgttttta agcgattccc acggcgttaa
     4201 gcaatacaac tgccaaccat cctaatccat aaccgatagt tgtaaaagct acttgttgga
     4261 tcaaaggcat tttccacgag cctgtttctt tgcgcacaac ggcgagcgta gatgtacatt
     4321 gtaatgcaaa cataaaaaat attacaatgg ctacgaccga agaaaccgta aatacgggtt
     4381 tacccgtctg ctcgtgccgg gcggttctca tagaattaat caatgactgc tgcatattat
     4441 cttcatcatc ttccgcaatg tgaaatgtca gggccgtcgc actgacgaaa acttcacgtg
     4501 cagcaaaagc ggaaatcatg cccacgccga cacgccaatc ccagccaagc ggcattaatg
     4561 cgggttcgat ccatcgacca accatcgcgg catatgaaga gctggtgcgt tctccggcaa
     4621 tccactgctc gacatttccg gaagctttgg cttcgtcggt gatttcaggg tttgcattag
     4681 gaaaataggt aagcccccag atgatgatcg agcataatat gatcgtagta ccggcgtttt
     4741 ggatgtatga aactgaacgc tgaaaggtat tacgcagtat aaatcgtata atgggtttgc
     4801 ggtaagccgg caattcaagc ataaaactgg acggtgtttt ttccttaata aaacgactta
     4861 caatacccgc tacgagtgca ccattgataa ggctcacaaa ataaataccg gataggatca
     4921 atccgctcaa ccaaggacga tccgagggca tcaggaaacc taaaagcaat gcgtacaccg
     4981 gtaatcgcgc gctgcatgtc atcaacggta ttataaaaat cgtaagcagt ctttcccgct
     5041 gattggggat agtacgagcg gccatagtcg ccggtatcgc gcaagcaaag cctgaaagca
     5101 tgggtacaaa cgaacgaccg tttagtccga tcttggaaag cggtttgtca atgagcatgg
     5161 cgccgcgaga taagtaaccg gaatcttcaa gcatccccat gagtaaaaaa agaattacaa
     5221 tttgaggaac aaaggtcatc acggtaccca taccggcgat taatccgtca gcaatgaggt
     5281 taccaagcca atgagatgat gggagccaag attttgagag attggctagt acatcaaacc
     5341 cctctgaaat tacatccatc atcggctggg ctaaccaaaa aacagacgta aaaataagcg
     5401 tcataaaaaa aacaaaaaag aacatacccc aaatcgggtg tagaaaaatt ctatccaatt
     5461 ttagagatgt ttcatccggt tcgatggtac gcgtattaaa tacagcgtga ttgatagcgt
     5521 ggatgtcggg tttgacagaa aaaggtcgta tcacttcttt ctctatggat tcagttattc
     5581 ggtacaattc cattaccgtt gattctgatt ggagcttggg gataggaggt aatatgtatt
     5641 tttgcgggac tgcaaaaaaa ccacgtaggg cttcagctaa ttcagccacg ccgttttcag
     5701 tgtgcccgta cgtttttata acaggagttc caagtcgttc cgatagcaaa gttgtatcta
     5761 tcgaaaaacc gcgtttttgg agtaaatcat tcatcgtaag agcgacgatg accggaaagc
     5821 ctgcatctat aagttgctta accagataaa gttggcggga gagctgagtg gcgtccgctg
     5881 taacgaggac aaggtcagga tgtcggtaat tgggatctaa aaataacgag tcgacagaaa
     5941 ctctttcgtc cagcgactgc ggagttaagc tgttgatacc gggcgaatca ataacttggg
     6001 cgtcacattt aaaacgctcg tgcaaacgac caacaagaaa ttcgaccgtc gtgcccggat
     6061 aattagaagt tttaaaatgt gagccggtaa ggatattaaa taaagtggtt ttaccggagt
     6121 taggttgacc gataagtgta atgcgaaagc tagcgttgct gctggtgccc gaaacagagt
     6181 tcgaagatgg cgtatgacaa tccgatggca aaaccgaatc cgtcatatat gaatgcattc
     6241 cgcttcattt ttacgaagcg ctattactgt accccgaaca gaaaccgcca tagggcatcg
     6301 aaaaggggac ttggcgatta ctttcacttc ttgtccaggc gtaaatccca tttccatcaa
     6361 acgtatacat gtgctatcgt tttcaagatg ttcgccgctg attttacaaa taacagcaca
     6421 ttcgccgcat ccgagttgtg ctacagtttt caatttgttc gacgtcatag ctaaagtttc
     6481 tttaatgttt aataatttca ttaagattga gactgcgtct caatcttaat gaaaagaata
     6541 tagaataata cagtcaacca gtcaagagaa acaatgatat acgtcaggaa aatgcttgac
     6601 aagggaattt ttttattcta cattagtttt attgaaaaca cctaattata gatcaaacag
     6661 tggtttaagg agataatatc atgacagaaa ctatggaagc cgttaacgac gtggtgatta
     6721 caccgcgtgc tatcgaagaa atcggttcga tcatgcgcga acaaagtatt cctgaaaatt
     6781 atgctttgcg tatgggtgtt caaggtggcg gttgctctgg ttttcaatat tcgttagctt
     6841 ttgatgaaac catttctgaa aacgatacga tattggaatt cggcgcagtc aaagttgccg
     6901 ttgattacaa gagtatgtta tatctcaaag gcgtcactct tgactttcaa gatgggttaa
     6961 acggacgcgg ttttgttttc aataacccta atgccaaaaa aacgtgcggc tgcggtagtt
     7021 cattttctgc atagatttcc agtagtttta tataaaaccc gatacttcag tgtatcgggt
     7081 tttttgtttt ttgatgcgta ctacatcata atgcaggtgc tctgatcgcg ataaccatcc
     7141 atattgtttg aatcagcaaa caaaacaata tgagggaaaa atgaaaagat ttcattgtat
     7201 taagagggtt gtagtttttt cttttattat gggtttatta gggttgttat cgtgtgatga
     7261 cgatagcgct aaagacacag atgatggtac tgcaaataac gaaccggggc ttgtggcgca
     7321 ctacccatta gatggaaatg cagatgacgt tggcaattcc catagcgatg gcgttgtttc
     7381 aggggcagtt tcggcaaatg atcgtcacga ccaagttgga aaagcaatgt attttaatgg
     7441 gaactctgat tttatcagtg tcaatgatac ttctaatttt caatttgacg attcgctgac
     7501 cgtaacggca tgggtgagaa cagattcact gaaaagccaa attgtcgttc gtaagggagg
     7561 cggtttagca gttcctgtgt ttgaattata ccttgccggt aatggagatc gcggatttcg
     7621 cattggaaca aattttacat acaaaagcgg agggtatcct ttacaaacct ggatgcattt
     7681 ggccggtact tatgatggcg aatctatggt tttttatatt aatggttcac cggttgatac
     7741 gttacaaaaa agtaatgcat tgaccagcga taaaaatctg ctattgattg gaacgcgtac
     7801 aggtcttaac gcagatacat tcgcagggcg tctggatgat atcagaattt atagccgctc
     7861 cctatctttg agcgaaattg cagcgatggc cgcggaatga ttaaacctct taacgcttac
     7921 tctgtctaac aataaaatga cgaaaggagc cgcgttatga gagaaattcg ctggatcgca
     7981 atggtgtctc tgtttttttt aaccggaagt gtcgtgtggg gtcaaactga aaattctgaa
     8041 cctccggatg atgccgcttt gaagcatgca gtagaaaata aaattcaagg tatcgtatgg
     8101 tatggagcac gtgactatgt aaacgtcgaa gtatcggagg gaacagtaat attaaacggt
     8161 tgggttagtg ggtattggaa aactgataaa atccaaaaag cggtagagca ggtacccggt
     8221 ataaaggaaa ttacgaatga aatcaccgtt tccaatggat cagatgaatt ggccgggcgc
     8281 gcgttaagtg cgatttatcg tgacgccatg ttcagaaaat atgcattggc tgccgacctt
     8341 ccggtacatg tgatcgtgca aaataattcc gttattttag ccggcaaagt gatggtcgag
     8401 tccgaacgcc gacgtgcaga gtttttagtg gacgctcata ctgccgccgc cgcaattgat
     8461 aaccggttag ttatcataga ctagttttaa tttgctaaaa tcaccactat tggtgagttg
     8521 atctaaaaac gcataaacgt attttgaagt tatacctcag gaggcgttta tgcgtttttt
     8581 tattttaatt ttgatgattc cgatcgtcaa ttttgcagtt tctcaaacac aagtggatat
     8641 tcctgaaaaa gcggaagcgg cttataaggc ttcagactgg gcaacttccg taaaattata
     8701 tttgcagttg tgcaagagtc aaccgggtga atcaaaattt tggaatcgtc tcgcgacgtc
     8761 gtattatcaa ctaggggcat ataaaaacgc gtctgagaca taccgtcaat acttatcttt
     8821 gactcaagac cctgtcggca tgtataatgc ggcttgttca tttgctttat ccggcgaaaa
     8881 ggatacagcg tggtattggc ttgaaaaagc gacccaaagc ggatggttca cgtcattgca
     8941 ggtacgccat gataacgatt tgaaatcact ccattccagg aaattcgatg acttagtcat
     9001 atcattagat cgagctgcaa gaccttgcga atatgattca ttgtttcgtc aatttgattt
     9061 ttggatcggt gaatgggatg tgtttaatct cgccggacaa aaagcaggta cgaatacgat
     9121 tcaaaaatta accgatggtt gtttattgtt cgaaaactgg accggttcac tgggtggttc
     9181 aggaaaaagt attaattttt atgataaaca gaaaaaacta tggcgacaga tatggatcgc
     9241 aagcaatggc aatgtttctg atttttatgg gcagtattcc gaaggtgtaa tggattttta
     9301 cggggaatcc gtttcggcga atgggagtat aggaaaaaat agattacgtt tttttaagat
     9361 tgattccgat acagtacgcc aatttgcgga acgttccgat gataacggaa aaagctggca
     9421 ggtaacctat gattttcgtt atgttcgtaa gaaatgacgt tgagtcccgc cgatcacaaa
     9481 tcgaatatgt ctaatgaaat atttaatctt atttctttta ccggcttttc tttgtgcgca
     9541 gaacaatcgt attcgtttgg aaagccttcc gttagacgat gggttatcgt atcagtccgt
     9601 atatgcactt tatcaggact ctcgaggcta cctatggatc ggaacgatca gcggtctcat
     9661 tcgttatgac gggcgtaata ataccgtttt tcgtcatgag ccggaaaatc cacactcttt
     9721 gtctcatgac gacgtagtag ctatttacga agatgaattc aaaaaaatat ggattggtac
     9781 ttacggcggc gggctaaacc gatacaatcc tgagaccggt gtatttacac gatacctcaa
     9841 cgattcgacg gatacaaaat ccatcagcga taatgtcatt ttatcaataa ccggtgataa
     9901 aaatggaagg ctatggatag gaactcgcac cggcggaatc aatgtattga acataacttt
     9961 acccgattcg ggttttacac gatttacgcg cgatccgaaa aagccgcgcc gaacagcgcc
    10021 acaagcaccc caaaagttat atacctcatc cgacggtcgt atttggattg gcgcatttgg
    10081 gcgaggattg gtgacatatg atcaatcgga aaatggtttt agatattttc cattaactcg
    10141 gaattccggc gatacagtga ggttttataa tgcgtacgat attgtagaaa ttaataattt
    10201 actgtggatt tcaacggatg tagggttgaa aacatttgat ctaaaagttg atcaatggat
    10261 cgaatcaact tttccaacaa aagcgacatg cgattcgtta tcggccttta gattagctgt
    10321 cgatagaata aataatgtgt tgtgggtcgg gtcgagcgga aagggtttgt tcaaatttga
    10381 ttttaattct aacaccatat cacaaaataa aaatcattgg tcttatgcgc cgtatgctct
    10441 aaaaactgat atggtgacgg ccttgcttgt ggatcaatcc tccatgttgt gggttgcaac
    10501 ggccaatggg gcattacaaa aaaatgcacc ggatttttat aagtttgacc gacttacaga
    10561 cctgtttgat cacatatctg gtgaaatgta cgtcaatgcc attttgcctg attcgtcgag
    10621 cgtttggata gggcatcatg gaggattggt tcgaataaac aaaaaaagta ttgaaacatg
    10681 ggaagatatt tttccggcat ctcatcaaag cattcgttcg gtaactgcac tttggaaaga
    10741 tagtgatggg gcgctatggg taggcactac aacagccggg ttgattcgta taaaaaacag
    10801 aattaaaaca acgtttttaa tatctcctaa agaaaatact agttattctt ttccgatatc
    10861 ttccatcatt agagataaaa agggacaact ttgggttggt acgattgacg gcgggctgaa
    10921 ccttttcgat gaaatcgata aatcatttat tcgttatgat cctgccaatt cgatcatgaa
    10981 atcagcctct gtaagttgtt tgtatgaatc gagcgattcg actatgtggg ttggaaccta
    11041 tggcgggtta ttgcaaattt cacaagaagg gccgtggaaa tattttacac atgatttaaa
    11101 gaatccaatg tccatttctc ataattacgt ttattcggtt ttttgcgatc aaaatggaag
    11161 aacctgggtc ggtacaaaca atgggctttg ttataaagaa aagaactctg acgatttcaa
    11221 agttcttaac caaaagaacg gattaccgag cgatgtaatc gtcggtattt taggaagctc
    11281 tggtggaaaa atttgggtca gtacacataa cggcatcagt agttttgatc ccaatcaacc
    11341 gacttctttc agaaattacg ataaatatga cggtttgccg ggaaatatgt ttaatatcgg
    11401 agcggctata caaagccgcg atggcacgtt atggtttggt agcatccaag gtgcagtttg
    11461 ttttaaatca gatcaactta gggataattt atttgaacca caggtgcaga ttgttcaagc
    11521 caaaaaattc aatcaaataa tttcgattga ttcggatttg attagactca actacgatga
    11581 aaattatctt tcgatttcct tttctgctat ggattttttc gctccgaaca aaaatcaata
    11641 tcaataccgt cttgagcctt tggaaaaggc ttggcattat gcgggaaaca agggtgaagc
    11701 acagtatacg gacctcgctc cgggatcata cacacttacc gtacgcgcta cgaatcgaga
    11761 tgggatttgg agttcgcaga tcggtgcagt acaagttatt attcgaccgc cattttggaa
    11821 aacaagaagc tttttgttcc ttgttgcaat gacagctttc ctgttaattt ggtttgtgca
    11881 ccgtagtatg acgaaacgcg ctgttgacaa atccctcgaa atggagcgta tccgtttaac
    11941 cgaacgagag cgcatgcgag aacaaatatc caaagatttt catgatgaat tggggcataa
    12001 gcttacaaaa ataacgcttt ttagtgagtt atctcaacgt gctcttcatg ataataaaac
    12061 cgattcggaa aaatttttac gaaaagtggc tgatacgtcc acttctttat ctgttacaac
    12121 ccgagatttc atttgggcat tagatccggg aaaagactcg ttgtatgatg tgatagttaa
    12181 tcttcgtgat tttggattcg atctcgtaca agatactgag atagtttttg aagtcggcgg
    12241 gcttactgag caacttgatc gaatccgttt aacgatggaa tggcggcgtc atttgacctt
    12301 aatcttcaaa gaggctatga acaatgcctt aaaacatgca aaagcaaaac acattcgtct
    12361 tcgtgtaatt atcgaaggga ttcactttgc gattcattta accgatgatg gtaccggctt
    12421 ttcacaggag agtactcgtt caacgcgcaa tggcttaaaa aatatgcaag aacgcgccca
    12481 aaaactatat ggtttactga gtatagaatc aagcgaatca gcgggtacgc acatatcctt
    12541 taccggttta ttaccaacaa ttgatgccga agcacgcaat tcccaggaaa aacgtgattg
    12601 gataactaca atcaagggat ggtttgtatg atacgactca tgatcgttga agacgatccc
    12661 gatatacgtg aaggattgga gcttatcgcc attcagacgc ggggtgtaaa actcgtcgga
    12721 gcttataaca ctgcagaaac cgctatagaa gcaactgaaa aggacaaacc agatgtactt
    12781 ttacttgata ttgaattgcc gggaataaac ggaatagagg ctattccgct ttttaagaaa
    12841 cgtaacgaaa atactgatat acttatttta accgttcatc aggacgatca aacggtattt
    12901 gatgctttgt gtgccggggc atcaggttat ctgatcaaaa cagcccatcc taataaaatc
    12961 atggatgcgg tgaaagaaat aaactccggt ggcgtaccga tgagtgcgtc tatcgcacgg
    13021 ctcgtaattc aatcttttca aaaaaaacaa aatccggttt taacaacacg ggagacagat
    13081 gtattgcggt ttctttgtaa aggccacagt tataaaatga ttgctgatag tatgcacgtc
    13141 agctgggaaa cggtgcattc acatatcaaa agcatctatc gtaaacttaa tgttaactcc
    13201 aaatccgaag ctgtcatcac ggcgttaaaa gatcgtataa tttgagttct cgtcgatagg
    13261 ttgccgttct ttatttagtg taaatacttc cacacattaa ttcaccaata ttggttacat
    13321 ctttacgctt ggaacttcct taggtttgta aaaggaaaat taattacaaa cgtaggtgag
    13381 gtgttatgcg taaaatttta ccgttattct ggatcatatt agtgatggtt tggccggatc
    13441 gttctttgtt gggtcaaatg cgcttcgtac ccattaccga tacatctaat cccatcaata
    13501 cagatccgct aggacctgca gtgaataact acaatggctc cgcatggatt gattttgacg
    13561 gtgatggtga tttggatttg tacgcccaca ttgggcggct ttatcgtaat gatggaggaa
    13621 atttcgttgc cgtgacgaat agccccggta gcggtttatc caatgcggca gttggtaagg
    13681 ggatttcttt tggcgacgtg gataatgacg gtgatcttga tgctttcgtg acttccacgc
    13741 gcggttcagg gttatatttg aataacggcg gcacttatac acgtattgcg acaggatcga
    13801 taggtcaagc taatgccggt tgggccggcg
//
LOCUS       DAOQNQ010000055        13712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1589_length_13712_cov_5.657099, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000055 DAOQNQ010000000
VERSION     DAOQNQ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13712
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1589_length_13712_cov_5.657099"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..1055)
                     /gene="cdaA"
                     /locus_tag="PK754_05430"
     CDS             complement(225..1055)
                     /gene="cdaA"
                     /locus_tag="PK754_05430"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNB08977.1"
                     /translation="MQDELFKIWTISVTAIDLLDIAAVTIIFYRAYLVIQGTTASRML
                     MGLIFILILSIAVQALNMSGMIWLVDQIRTVWVIAFVIIFQPELRRLLLFFGQSGFLR
                     NIIKLGIPEYIEEIIKACELLAKRRNGAIIAIERSTGLRNIVETGLHLEANISQQLLL
                     SIFNPRSPLHDGAVIIENDQIAAAKCLLPLSPNPYLETSVGTRHRAGVGLSEQSDAIV
                     IIISEETGKISVAFKGVLHRNLTPSELRGFIEEVITSGKTRGLWRRIRRQPRKTLVNN
                     "
     gene            complement(1057..1884)
                     /gene="folP"
                     /locus_tag="PK754_05435"
     CDS             complement(1057..1884)
                     /gene="folP"
                     /locus_tag="PK754_05435"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNB08978.1"
                     /translation="MSNNSEALYSALKTRVHVMGILNVTPDSFSDGGRFFSTEKAVVH
                     AEQLIADGADILDIGGESTRPGAKPLTLKDELDRVLPVLRAIRKKSDIPISIDTYKTD
                     VAKAALDEGADLINDISGMTMDEGIAHIAAQNDVPLVIMHIRGTPSQMQNNPSYGDVV
                     KEVKQFLETQCTKAQQMGACKLIIDPGFGFGKRYEDNFTLLKNLRTFTALGYPLMAGT
                     SRKSFLGKAFDAPPANRLEGTLISNIYAVQQGATLLRVHDVSSMKRALHIHQMIETA"
     gene            complement(1874..3553)
                     /locus_tag="PK754_05440"
     CDS             complement(1874..3553)
                     /locus_tag="PK754_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08979.1"
                     /translation="MDKILYWLSGGVPGGFGIWFAVVAALCFIWWFLHRYSPVVNHSG
                     LARKMMIVLGLIVLTYTTIRFARPPKPFNVYVGILPLENQGSNKSLSVAGTGWGLAEI
                     TTHHAALGSPEHLHFLRPEWLVESYFGDSTSAISPSNKQKLFRWARLIKLNYLVAGFY
                     NADANPVSMSYEVYDVLTGETMRSFTNTIPTGDEVPYGQAMRTAAEELMTYLYEVTDE
                     EFNSAADHTDLFQTKSLPLYFSARDLLAHEQWDSAFAVARMALQKDSASVLGWFAAGS
                     AYTEKMIREKDARAREMLQRRGEYHLKRGAQINDQYQPILMSLARNFSYTKPEPRYLD
                     AEFAMIASQAIYKRDYQLYYILSFMQRMRWETFEMKSREEILNRAIEINPASFDAYVA
                     LGQDFLERSRPHDHLSQYALNNFNVALQLKPNDFESIMGMVRACDYLAYFDKAVELLE
                     RAEKFYPDRADVYYTYGVIYYHKGEYIREKTKLREGHEQFPIAEGYFKKAVQLNNSPY
                     AWLYLGKIYDYQKRRNEAIEALRTVMKLLKPDDRYREEARKKLREYFPDVE"
     gene            complement(3645..5627)
                     /gene="ftsH"
                     /locus_tag="PK754_05445"
     CDS             complement(3645..5627)
                     /gene="ftsH"
                     /locus_tag="PK754_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNB08980.1"
                     /translation="MENQNAQKNDAPKPGPTKKKNRPSPDSGPNWKGTIGTFFLWIAI
                     FGSFFFLYQIVSAGTDKHIELSFTQYQFYLEQDKIRTITVGEGELRGTLKAEETITTD
                     GTPYRFTQFRINLPFVDSKMLDEWKQRGVDVEFMKRGTDFGTIILQMLPWILAIAILI
                     FIFRRMQGGPGGKNIFTFGKSRARMSTDKDHKVTFTDVAGCEEAKQELSEIVDFLKDP
                     NKFTRLGGRIPRGALLLGPPGTGKTLLARAVAGEAEVPFFSISGADFVEMFVGVGAAR
                     VRDLFEQAKRSHPCIIFIDEIDAVGRSRGAGLGGGHDEREQTLNQLLVEMDGFEENSG
                     IIILAATNRPDILDNALMRPGRFDRQVVVDYPDVKGREEILKIHCRKVPISDEVKIEK
                     IAKGTPGLAGADLENLVNEAALLAARRNSDKVENIDFENAKDKVMMGVERKSMVISDK
                     EKRSTAYHEAGHALVGRLLPESDAVHKVTIIPRGRALGITHYLPSDDRHSYSRTYIEA
                     RLAMMMGGRAAEKIIYNHYTTGASNDIERATEMARRMICEWGMSDALGPVAFGKKDEE
                     VFLGREIATSKNYSENTAVQIDAEIKRIVSEAENKALSLIEQNIQTLHNIANALVERE
                     TIDSEELDMILKGQQLPPLEILNNGQSAAKIEAPVA"
     gene            complement(5641..6189)
                     /gene="hpt"
                     /locus_tag="PK754_05450"
     CDS             complement(5641..6189)
                     /gene="hpt"
                     /locus_tag="PK754_05450"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNB08981.1"
                     /translation="MNPEKVIVTESHRFTVMISESEIRDAIVRLGKKITTDYKDKNPI
                     LVGVLNGSFLFVADLIREIATECEVDFLKIGSYGDAMHSSGSVRLDKDVSAKLEGRHV
                     IVAEDIIDSGLSVTYIRNHLLSKKPASLAFASLLMKREKAKIDFPVEYVGFEIGNEFV
                     IGYGLDLGQRFRHLKDIYVTKD"
     gene            complement(6179..7582)
                     /gene="tilS"
                     /locus_tag="PK754_05455"
     CDS             complement(6179..7582)
                     /gene="tilS"
                     /locus_tag="PK754_05455"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNB08982.1"
                     /translation="MKTIEFTAWFEQRFFQNIHTKKLISKNHHILLAVSGGVDSMVML
                     RLFRRFAPELKLQLAIAHVNHGLRGKSAQDDAAFVKAIARLYGMPFYETRVHAKQHAR
                     RNKLSVEEACRVLRYDFLENTASTYGFDHVATAHHADDQAETVLMRIIKGTGIQGLSG
                     IPEVRGKYIRPLLPFTREEIHRYARENRIPFVTDLTNEDTTFLRNRIRHQILPDLRTI
                     NPQISHHLSRLADIAKSAADVLQMDAKRCYTKVFRTQKGKIWLEIQAFNRYYPIRQYT
                     LLQMFFASHAPWFTLTSVEYERILDLIQHQQPGRKFDAGKWRWTKTTRAVVITPVDTS
                     SRTKDVTLTIGNTVSWGSWKIRSSAERYTDTIRKNLAKNPYIVYLDAGPVTKPIRVRA
                     WKNGDRFRPYGMHQFKNVSDFFVDHKIPVTDKHTIPVLTHGRDIVWIGGMRTDDRYKV
                     TPDTLTVIKLELITHEP"
     gene            complement(7579..8565)
                     /locus_tag="PK754_05460"
     CDS             complement(7579..8565)
                     /locus_tag="PK754_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNB08983.1"
                     /translation="MIEFRDPYFLLLLLLIPILIFWNWKRRRRSALRYATVALIRDVP
                     RSWRLRIKPLHYILQYAALGLLVITLARPRLQDTQSVITAEGIDIMMAMDISRSMLIE
                     DLGKVNRLEAAKSVAEKFVSGRQSDRIGLVLFAGKSFTQCPLTVDYTMLTQLIKQVQI
                     GVVEDGTAIGMGLVNSINRLRESKAKSKVIILLTDGDNNRGEIDPLTAAQIAQAMNIR
                     VYTIGAGKDGIARIPVDDPFFGRQYLTAEVKINETPLREIADMTGGKFFRATNQKALE
                     EIYDQIGQLEKTKVDVKTYQRYRELYLWFLIPALVCMLTVVVLSRTVFFSLP"
     gene            complement(8562..9587)
                     /locus_tag="PK754_05465"
     CDS             complement(8562..9587)
                     /locus_tag="PK754_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNB08984.1"
                     /translation="MIKFANPEYLYALAIIPLLALLLWDWFRTQRLRMLRVGDTRLVD
                     HLSQRIDPKRRLARWTMWTAGMIFGIFAMAGLSVGSRYEKVKITGVDMVIALDISASM
                     GAEDIKPNRLEKAKHEIGTLLSRLGGDRVALVIFAGQAFIQCPMTSDYSAVRMFLDAI
                     DVQSTTAGGTNFAEMLEIARSAFPKPQDVQGPATGKAIVIFSDGEDHSDEATEKAKAL
                     LGENIRIFAVGVATENAVPIPVYDDRTGQTDFKKFNGSVVTTKREDAMLTAITDMSGG
                     AYYQATINETEIQKIYDAISQLQKGESAEYQFTEYENQFQWFVGLSLIAVVFYIGIGN
                     RKTESRA"
     gene            9856..10620
                     /locus_tag="PK754_05470"
     CDS             9856..10620
                     /locus_tag="PK754_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNB08985.1"
                     /translation="MRLISWNVNGIRAVSKKGFLEWLDKESPDVMCLQETKAMPEQLD
                     ASLLNPMGYTGYFNSAQRKGYSGVATFCLKKPTSVQNGIGVERFDAEGRVLMTDHGDF
                     ILFNIYFPNGKMNAERLQYKMDFYDTVLKYFDALKDQGKKLVICGDYNTAHKPIDLAR
                     PRENEKISGFLPSERAWMDELVSHGYVDTLRVFNQEPGQYSWWDQQSRARERNVGWRI
                     DYFFVSDNLLPALKNAWIMPDVMGSDHCPVGIELKF"
     gene            10636..11508
                     /locus_tag="PK754_05475"
     CDS             10636..11508
                     /locus_tag="PK754_05475"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNB08986.1"
                     /translation="MKNRFSIKDLEQFSGIKAHTLRIWEKRYDMLNPNRTKTNIRWYS
                     SDDLKRLLNIALLYHHDHKISKIAALTDKELADKVAEIAQNTSDYSVFVDGLVMAMIE
                     MDEQRFEKIFSSGLIKLGFEDTIAKVVYPFLIKIGFMWQMGSSNPAQEHFVSNLIRQK
                     IIAAIDGQVVPMDRSPKKAILFLPDGEMHELGLLVLNYMMRARGLNTYYLGQSVPMED
                     LRRACEIKKPDYLVSVFTSPMPKLKEFVRLLSQHFPHTKIILSGKQILSANLKLPSQF
                     YLLPSFDQLNKHLS"
     gene            11659..12393
                     /gene="folE"
                     /locus_tag="PK754_05480"
     CDS             11659..12393
                     /gene="folE"
                     /locus_tag="PK754_05480"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNB08987.1"
                     /translation="MRTQPDFRIPLQVLNNTTAGAREENGHIDLIEDDFGDDHISSAV
                     ETPMRDDAFALNDEAKIELIEYHFKKIMHVLGLDLTDDSLKGTPRRVAKMYVQEVFQG
                     LNPKNKPSVTLFKNQYHYNHMLVEKNITLFTYCEHHFVPIFGKVHVGYIPASQVIGLS
                     KINRIVQYFAKRPQVQERLTVQIAEELRRILNTEDVAVVIDAAHLCVSSRGVQDTEST
                     TTTSSFLGRFHDEGVRNEFLCYIELK"
     gene            12420..12992
                     /locus_tag="PK754_05485"
     CDS             12420..12992
                     /locus_tag="PK754_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009053398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNB08988.1"
                     /translation="MKQIIFLIVSINVLLFGIACRDVRIKPEQPNMKSTQSVYNIAVK
                     TIDGAETNLATYRGKKILLVNVASECGYTPQYKDLQKLQEQFGSKVVVLGFPSNDFGG
                     QEPGSNAEIKNFCERNYKITFPMFDKIAVVGEGQHPLYQWLSQKNLNGWNEKAPSWNF
                     CKYLIDENGELIGYYNSSVNPLSDEIINKL"
     gene            13005..13466
                     /locus_tag="PK754_05490"
     CDS             13005..13466
                     /locus_tag="PK754_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNB08989.1"
                     /translation="MHVLEREQTLSCTLLDAWQFLSSPQNLKLITPPYMGFEIRSDQN
                     GKPMHAGMIITYRVSPLFNIPMTWVTEITHVREPYYFVDEQRIGPYTLWHHQHFLEAV
                     EGGVRMRDCVHYKLPMGIIGNQIEKWIVRRRLESIFDFRWRKLNEIFNAPS"
BASE COUNT         3634 a         3100 c         3215 g         3763 t
ORIGIN      
        1 gatggaatag agaattcata gattaattcg cccggctctt gttttatgac ggtggcgtaa
       61 ccgcgagggg aaaccatgtc cgtcgtttgt gcggttaaaa aatttgtgct gcacaacaga
      121 ctaattacta aaacaaaatt tttattcatt cggtgcgtat tcgcaaaaac aaaatccata
      181 gtgtcggtta atatgtatat tgtacggtaa aacagagatt tcggttaatt atttacaagc
      241 gtttttcggg gttgacgccg gatacgacgc cataggccgc gcgtttttcc ggaggtgatc
      301 acttcttcga taaaaccgcg cagttccgac ggtgtcagat tgcgatggag tacgcctttg
      361 aatgcgacgg atattttccc ggtttcttcc gaaataataa tcacgatcgc gtccgattgt
      421 tccgacagcc cgactcccgc acggtgacgt gtaccgaccg aagtttcaag gtaaggatta
      481 gggctcagcg gcagtaaaca tttggccgcg gcgatctgat cgttttcgat gatgacggcg
      541 ccgtcatgca gcggtgaacg gggattaaaa atcgaaagca aaagttgttg agagatattg
      601 gcctcaagat gaagtccggt ttctacaata ttgcgcaaac ccgtggagcg ctcaatggca
      661 atgatggcgc cattgcgccg tttggcgagc aattcgcaag ctttgatgat ctcttcgatg
      721 tattcgggga ttccaagttt gatgatattt cgcaaaaaac cgctttggcc gaaaaaaaga
      781 agtaagcgcc ggagctccgg ttggaaaata atcacaaagg cgataaccca aaccgtacga
      841 atctgatcca ccagccagat cattccgctc atattgagcg cttgcacggc aatggataaa
      901 atcaaaataa atatcaaacc cataagcata cgtgaggccg tggttccctg aatcaccaaa
      961 taggctcgat aaaaaataat cgtcacggcc gcaatgtcca gcaaatcgat ggccgttacg
     1021 gaaatcgtcc atattttgaa aagttcgtct tgcatgtcag gccgtttcaa tcatctgatg
     1081 aatatggagc gcacgtttca tagacgatac atcatgtacg cgcaataggg ttgcaccttg
     1141 ctgtacagca tagatattgc tgatgagcgt accttccaac cgattcgccg gcggcgcatc
     1201 aaatgctttt cctaaaaatg atttgcgcga tgtgcccgcc atcaaaggat aacccagcgc
     1261 cgtaaaagta cgtaaatttt tgagtaatgt aaaattgtct tcataccgct taccaaatcc
     1321 gaaacccgga tcaatgatca atttgcaggc gcccatttgt tgggctttgg tacattgggt
     1381 ttcaagaaac tgttttactt ctttgaccac atcgccatac gatggattat tttgcatctg
     1441 gctcggtgtg ccgcgtatgt gcatgatgac cagaggaaca tcattttgag cggcgatatg
     1501 agcgatgcct tcgtccatcg tcatcccgct gatatcgttg atcaggtctg cgccttcgtc
     1561 caatgcggcc ttcgccacgt cggttttgta ggtgtcaata gaaataggga tgtcggattt
     1621 tttgcgaatt gcgcgaagca cgggaagaac gcgatcaagt tcgtctttca gtgtcaaagg
     1681 tttcgcaccg ggacgcgtgg actctccgcc gatatccagt atatcagcgc catcagcgat
     1741 aagttgttcg gcatgaacga cggctttttc tgttgaaaaa aaacgaccgc cgtctgaaaa
     1801 agaatcggga gtcacattga gaatacccat gacatggacg cgcgttttta gagcgctgta
     1861 tagggcttcc gaattattcg acatcgggaa aatactcgcg gagtttttta cgcgcttctt
     1921 cgcgataacg gtcatccggt ttgagcaatt tcattaccgt tcgtaaagct tcgatggctt
     1981 cgttgcggcg tttttgataa tcatagatct taccgagata cagccaagca tacggcgaat
     2041 tattgagttg cacggctttt ttgaaatacc cttcggcaat ggggaattgt tcatgccctt
     2101 cgcgcagttt tgttttttcg cgaatgtact cgcccttgtg atagtaaatc acaccgtagg
     2161 tataataaac atcggcacga tccggataga acttttcagc acgttccaac agctcgaccg
     2221 ctttgtcgaa gtaagccaaa taatcgcacg ctctgaccat acccataata gactcgaaat
     2281 cattaggttt gagctgtaat gcgacgttaa agttattcaa tgcatattgc gaaagatgat
     2341 catggggtcg gctgcgctcc aaaaaatctt ggcccaaggc aacgtatgca tcaaaagacg
     2401 ctggattgat ttctatggcg cgattgagaa tttcctcgcg ggatttcatt tcaaatgttt
     2461 cccaccgcat gcgttgcata aaactcaaga tgtaatacag ctgataatcc cgtttgtata
     2521 tggcttggct tgcgatcatc gcaaattcgg cgtccaaata acgcggttcc ggtttggtgt
     2581 aactgaaatt gcgtgccagg ctcatcaaaa tgggttggta ttgatcattg atttgcgcac
     2641 cgcgtttgag atggtattct ccgcgacgtt gaagcatttc gcgcgcgcgc gcgtcttttt
     2701 cacgaatcat cttttctgta taagccgaac ccgcggcaaa ccatcctaaa acggaagcgc
     2761 tgtctttttg taacgccatg cgggccaccg caaaagcaga atcccattgc tcatgcgcca
     2821 aaaggtcgcg tgccgaaaaa tataaaggta gcgatttggt ctgaaacaag tcggtgtgat
     2881 cggcggcgga gttaaactcc tcatccgtaa cttcgtataa ataggtcatc aattcttctg
     2941 cggccgtccg catcgcttgt ccgtaaggca cttcgtcacc ggtggggatg gtgtttgtaa
     3001 aagaacgcat tgtttcgccg gtcaacacat cataaacctc ataactcatg ctaaccggat
     3061 tggcgtcggc attataaaaa ccggcaacga ggtaattcag cttgatcagg cgcgcccatc
     3121 tgaaaagttt ttgtttgttg gatggtgaaa tggccgaggt cgagtccccg aaatacgatt
     3181 cgacgagcca ttccggacga aggaaatgta aatgttcggg tgatccgagt gcggcgtgat
     3241 gcgtcgtgat ttcggctaag ccccaacccg tcccggccac ggacaaactc ttgttggagc
     3301 cttggttttc cagaggaaga attcccacat atacgttaaa tggtttcggc gggcgcgcga
     3361 aacgtatcgt ggtgtacgtg aggacgatga ggccgagcac gatcatcatt ttccgagcta
     3421 atccggaatg attgacgacc ggagaataac gatgcaaaaa ccaccatata aaacaaagtg
     3481 ccgcaacgac cgcaaaccaa atcccaaagc cgccgggcac gccgcccgag agccagtaca
     3541 atatcttatc catatttttt cctacccgtt cgctattaga accctaagcg catacaaagc
     3601 ttggcgatta ttaccgccaa actgcaatca aaacaacatg gcgattatgc cacgggcgct
     3661 tcaattttag ccgcactctg tccattattt aggatttcga gcggtggcag ctgttgccct
     3721 ttgagtatca tatccagttc ttccgaatca atggtctcgc gctccacaag cgcattggca
     3781 atattgtgca gtgtctgaat attttgttcg ataagggaca atgctttgtt ctcggcttct
     3841 gagacgatac gtttgatttc cgcatcgatc tgtaccgcag tattttcgct atagtttttg
     3901 gatgttgcga tttcgcgtcc gagaaaaact tcttcgtctt tttttccaaa ggccaccgga
     3961 cctaatgcgt cgctcatgcc ccactcgcag atcatgcgac gcgccatttc cgtagcgcgt
     4021 tcgatgtcgt tgctggcgcc cgtcgtatag tggttgtaaa tgattttttc tgccgcacgg
     4081 ccacccatca tcatcgcaag tcgcgcttcg atatacgtcc gcgaatacga atgccgatca
     4141 tccgagggga gatagtgggt gatgccgagc gctctaccgc ggggaatgat cgtgactttg
     4201 tgaaccgcat cggattccgg caaaagacga cctaccagag catgaccggc ttcatgataa
     4261 gccgtcgaac gtttctcttt gtcgctgatg accatacttt ttcgttcaac gcccatcatg
     4321 actttatctt tggcgttttc aaaatctatg ttttcgactt tatccgaatt gcgacgcgct
     4381 gcgagcaaag ccgcttcgtt gacgaggttt tccaaatccg ctccggccaa accgggcgtt
     4441 cctttggcta ttttttcgat ttttacttcg tcactgatcg gtactttccg gcaatgtatc
     4501 tttaaaattt cctcacggcc tttgacatcc ggataatcca cgacgacttg acggtcaaag
     4561 cggccggggc gcatcaacgc attatccaaa atgtcggggc ggttggttgc cgcgagtatg
     4621 atgataccgc tgttttcttc aaagccgtcc atttcgacca gcaattgatt gagcgtttgt
     4681 tcgcgttcgt cgtgacctcc gcccaaaccg gcgccgcgcg aacggcctac ggcgtcaatt
     4741 tcgtcaataa atataatgca cgggtgactg cgtttggcct gttcaaaaag gtcgcgcaca
     4801 cgtgccgcgc cgaccccgac gaacatttcc acaaagtccg caccgctgat ggaaaaaaac
     4861 ggaacttcgg cttcaccggc gacggctctt gccaataggg tttttcccgt acccggaggg
     4921 cccagcaaaa gggcgccgcg cggaatacga ccgccaaggc gcgtgaattt gttggggtct
     4981 ttgagaaaat caacaatctc ggagagctcc tgtttggcct cttcacaacc ggccacgtcg
     5041 gtaaaagtga ctttatgatc tttatccgtg ctcatacgag cgcggctttt gccgaacgta
     5101 aaaatatttt ttccgcccgg tccgccttgc atacgacgaa aaataaaaat cagaatagcg
     5161 atggccagaa tccacggcag catttggaga ataatcgtac cgaaatccgt accgcgcttc
     5221 ataaattcaa cgtccacgcc gcgttgtttc cattcgtcta acatcttact gtccacaaag
     5281 ggcaaattga tgcgaaactg cgtaaatcgg tatggcgtac catccgtggt gatcgtctct
     5341 tccgctttga gcgttccgcg caattcgcct tcaccgacgg tgatggtgcg aattttatcc
     5401 tgttccagat agaattggta ttgcgtaaaa gaaagttcga tatgtttatc tgtgccggcc
     5461 gacacaatct gatagagaaa aaagaacgac ccgaaaatgg cgatccacaa aaagaatgta
     5521 ccgatcgtac ctttccagtt ggggccggaa tccggcgaag gacgattttt ctttttggtc
     5581 ggtccgggtt tgggggcgtc gtttttttgt gcgttctggt tttccaaaaa cagctcctta
     5641 ttaatccttt gttacataaa tatctttgag atgacgaaaa cgctggccga gatcaagacc
     5701 gtaaccgatg acaaattcat tgccgatctc aaaaccgaca tattccaccg gaaaatcaat
     5761 cttggctttt tcgcgtttca taagcaaaga agcaaatgcc agcgaagcgg gttttttaga
     5821 cagcaaatga ttgcggatgt acgtcacgga aagaccggaa tcaatgatgt cctccgcgac
     5881 gatgacgtgg cgcccttcca atttggcgct tacatcttta tccaagcgga cgctgccgct
     5941 ggaatgcatc gcatcgccgt agcttcctat tttcaggaaa tccacttcac attccgtggc
     6001 gatttcccgg atcagatcgg cgacaaaaag aaacgatccg tttaatacgc cgacaagaat
     6061 aggatttttg tctttgtagt cggttgtgat tttttttccc aggcgcacga tagcgtctcg
     6121 gatttccgat tcggaaatca tgacggtaaa acgatgggat tcggtgacaa taactttttc
     6181 agggttcatg ggtgattaac tcgagcttta ttactgtgag ggtatccgga gttactttgt
     6241 agcgatcatc ggtacgcatc ccgccgatcc acacgatgtc gcggccatgg gtcaaaacgg
     6301 ggatagtgtg tttatcggtt acgggtattt tatggtccac aaaaaaatca gacacatttt
     6361 taaattgatg catgccatac ggcctaaaac gatcgccgtt tttccatgcg cgcacacgta
     6421 taggttttgt aacgggcccg gcatcaaggt acacgatgta aggattttta gctaagtttt
     6481 ttctaattgt atcggtgtac cgttctgcgg aggatcgtat tttccatgat ccccacgaaa
     6541 ctgtatttcc gatcgtcaaa gttacatctt ttgtccgcga agaagtatca accggcgtga
     6601 taacgacggc ccttgttgtt ttagtccatc gccattttcc ggcatcaaac tttcgtccgg
     6661 gttgttgatg ttgtatcaaa tccaatatcc gttcatactc gacggaagtc agcgtaaacc
     6721 agggtgcatg cgacgcaaag aacatttgca ataacgtgta ttgccggatc ggataatagc
     6781 ggttaaaggc ttgaatttca agccaaattt tgcccttctg cgtgcggaaa accttggtgt
     6841 agcaacgttt ggcgtccatt tgcaaaacat cggcggccga ttttgcaatg tcagccaatc
     6901 ggctcaagtg atgcgaaatt tgtggattga tcgtgcgcag atcgggaagt atctgatgcc
     6961 gtattctgtt gcgcagaaaa gtcgtatcct cgttggtcaa atccgtgacg aatgggatac
     7021 gattttcgcg tgcataacga tgtatttctt cgcgcgtaaa tgggagcagc gggcgaatat
     7081 acttaccgcg cacttccggt attccggata agccttgaat gcctgtccct ttgataatac
     7141 gcatcagtac ggtttcggcc tgatcatcgg catggtgggc cgtcgcgacg tgatcaaaac
     7201 cgtatgtgct cgcggtgttt tctaaaaaat catatcgaag cacccggcag gcttcctcga
     7261 cggacagttt attgcggcgt gcgtgttgct ttgcatggac gcgggtttca taaaaaggca
     7321 taccatacag ccgggctatt gctttgacaa aggccgcatc gtcttgtgca gattttccgc
     7381 gaagaccgtg gtttacatgc gcgatggcaa gttgcagttt tagttccggt gcaaaacggc
     7441 gaaataagcg cagcataacc atcgaatcta cgccgccgga aacagccaat aaaatatgat
     7501 ggtttttgga aattaatttt ttggtgtgaa tgttttgaaa aaaacgctgt tcaaaccatg
     7561 cggtaaattc gatggttttc atggcaggct aaagaaaacc gtacgtgaca gcacgactac
     7621 ggttaacatg catacgagcg caggtatcag aaaccaaagg tagagctcac ggtaacgctg
     7681 gtacgttttg acatccactt tggttttttc taattggccg atctgatcgt aaatctcctc
     7741 aagcgctttt tgattggtag cacgaaaaaa ttttcctccg gtcatatcgg cgatttcgcg
     7801 cagcggggtt tcattgattt tgacttcggc ggtcaggtat tgacgaccaa aaaaaggatc
     7861 gtccacaggg atgcgggcaa tgccgtcttt tccggcgccg atcgtataga cacgaatatt
     7921 catggcttgg gcaatttgtg cggcggtcaa aggatcaatt tcaccgcggt tattatcgcc
     7981 gtccgtcagc aatatgatca ctttactttt ggctttactt tctcttaatc ggttaatgga
     8041 attaaccagc cccatgccga tggccgtgcc gtcttcgacc acgccgatct gaacttgttt
     8101 gataagctgg gtgagcatgg tatagtctac cgtcagaggg cattgggtaa aactttttcc
     8161 cgcaaaaagt accagcccga tgcgatcgga ttgacgtccg gagacaaatt tttccgctac
     8221 cgatttggcc gcttccaagc ggttcacctt gcccaaatct tcgatcagca tgctgcgaga
     8281 gatgtccatg gccatcatga tgtcaatgcc ttccgccgtg atgaccgatt gcgtatcctg
     8341 caaacgtggt cgcgccaaag tgataaccag taaaccgagc gccgcatatt ggagaatgta
     8401 gtgcagcggc ttgatgcgta gccgccagga gcgggggacg tcacggataa gcgcgaccgt
     8461 cgcataacgc agcgccgaac ggcgacgtcg cttccagttc caaaaaatca gaatcggaat
     8521 caaaagcaac agcagtaaaa aataggggtc gcgaaattcg atcatgcgcg gctttctgtt
     8581 ttgcgattac cgatgccgat ataaaataca acggcgatca gactcaaacc gacaaaccac
     8641 tgaaattggt tctcatactc tgtgaattga tattcggcac tttcgccttt ttgaagctgg
     8701 gagatggcgt cgtatatttt ctggatttct gtttcgttga tagtggcttg ataatacgcg
     8761 ccgccgctca tatctgtgat agccgtgagc atcgcatctt cacgtttggt cgtaacgacg
     8821 gatccgttga attttttaaa atccgtttgg ccggtgcggt catcgtacac agggatggga
     8881 actgcatttt cagtggctac accgacggca aatatgcgaa tgttttcgcc gagcagggct
     8941 ttggcttttt cagtggcctc gtcgctgtga tcttcaccgt ctgaaaaaat aacgatggct
     9001 tttcccgtag ccggtccttg cacgtcctgc ggtttcggaa aagcggatcg cgcaatttcg
     9061 agcatttcgg cgaaatttgt accaccggcc gtcgtggatt gcacgtcaat tgcgtccaga
     9121 aacatgcgca ccgcgctgta gtcgctggtc atcgggcact gaataaaagc ctgtcctgca
     9181 aatatcacta aagccacacg atctccgccg agccgcgaga gcagggtgcc gatttcatgc
     9241 ttggcttttt ccaaacgatt gggtttgatg tcttcagctc ccatggacgc ggagatatcc
     9301 agtgcgataa ccatatccac accggttatt ttaactttct catagcgact gccaaccgat
     9361 aaaccggcca tagcgaaaat tccgaaaatc attcccgccg tccacatcgt ccatcgcgct
     9421 aaacgtcgtt ttggatcaat tcgctgagac aagtgatcta ccaagcgggt atcgccaaca
     9481 cgcagcatgc ggaggcgctg tgtgcgaaac caatcccaaa gcagcaaggc taggagcggt
     9541 atgatcgcaa gtgcataaag atattccgga ttggcaaatt tgatcacagt gtccgtcgga
     9601 taaaattaaa ctaaaaatta aaagcggttt caatatatac ggatgaagtc aattttgcaa
     9661 aatcaaagcg tgtttaattt tttatttgaa attataaaca caatttttat cttcttcgcg
     9721 ccgcagcaat gcgttttatg tacccatccc atgtgtggag cgatgccgca agagatgcta
     9781 gaatcggtcg cttagcatgt tgacaaaaat ctataaataa aaagcacaga gatacaattt
     9841 taatagagga atcgtatgcg ccttatcagt tggaatgtca atggcattcg tgccgtatct
     9901 aaaaaaggtt ttcttgagtg gctggataaa gaaagcccgg atgtgatgtg tttacaggaa
     9961 accaaagcaa tgccggagca actggatgca tctctactta atccgatggg atacaccgga
    10021 tattttaatt ccgcacaacg caaagggtat agcggggtgg ctacattttg tttaaaaaaa
    10081 ccgacgtctg ttcaaaatgg tatcggcgta gaacgatttg atgccgaagg tcgtgtactg
    10141 atgacggatc acggcgattt tattttgttt aacatttatt tccccaacgg gaaaatgaat
    10201 gcggagcgtt tgcaatacaa aatggatttt tatgacaccg tgttaaagta ttttgatgcg
    10261 ttgaaagatc agggaaaaaa attagtcatt tgcggggatt ataatacggc ccataaaccg
    10321 attgatctgg cccgcccgcg tgagaacgaa aaaatttccg gttttttacc gtctgaacga
    10381 gcctggatgg atgaattggt tagccacggt tatgtagata ccttgcgtgt tttcaatcaa
    10441 gagcccggtc aatattcgtg gtgggatcag cagagtcggg cacgcgagcg aaacgtgggg
    10501 tggcgtattg attatttttt tgtcagtgat aatctgcttc ccgcactgaa aaacgcatgg
    10561 atcatgcccg atgtgatggg ttcggatcat tgtcctgtag gcattgagtt aaagttttaa
    10621 tttgatcggg cacgtatgaa aaatcgcttt tcaatcaaag atcttgagca gttcagcgga
    10681 atcaaagcac acactttgcg catttgggaa aaacgctacg atatgcttaa tcccaaccgc
    10741 acaaaaacca acatccggtg gtactcttcg gacgatctca aacgactgct aaatatcgct
    10801 ctgttgtatc accatgatca taagatttcc aaaatcgctg cgctgaccga taaggagcta
    10861 gcggataaag tcgcagaaat agcgcaaaac acctcggatt attcggtttt tgtggatggt
    10921 cttgtgatgg ctatgatcga aatggatgaa cagcgttttg agaaaatttt ttcaagcgga
    10981 ctgatcaagc ttggttttga ggataccatc gctaaagttg tttatccatt cctcatcaag
    11041 atcggcttca tgtggcaaat gggttcgtcc aatcccgcac aggaacactt cgtgtccaat
    11101 ttgattcgtc aaaaaattat cgccgcgatt gacgggcaag tcgtcccgat ggaccgatct
    11161 cccaaaaaag cgattctttt tttgccggat ggcgaaatgc atgaattggg tcttttggtg
    11221 ctcaattata tgatgcgtgc tcgaggttta aatacgtatt accttggcca atcggtaccc
    11281 atggaagatt tacgacgagc ttgcgaaata aagaaaccgg actacctggt atcggttttc
    11341 accagtccta tgcctaagct taaagagttt gttcgcctat tatcacaaca ctttcctcat
    11401 accaaaatca tactgagcgg taaacaaata ttatccgcca atctaaaatt accgtctcaa
    11461 ttttatctgc tgccttcgtt tgatcaatta aacaaacatc tgtcataagt atttttttaa
    11521 tgagggcttg tttatttgtt gaatttttgt ttaatgttta atttcaaatt ttaaacaaaa
    11581 ttattgacat tatccgaacc atatgatatt ttgacttagc tttttttaaa gctaaacatc
    11641 gaatatcaag gaattaatat gcgaacacaa cccgatttcc ggattccttt acaggtttta
    11701 aacaatacga cggctggggc acgggaagaa aatggtcata tagaccttat cgaagatgat
    11761 ttcggtgacg atcacatcag ttcggccgtt gaaacaccga tgcgtgatga tgcctttgcg
    11821 ttgaatgatg aggccaaaat agaattgatc gaataccatt tcaaaaaaat catgcatgtt
    11881 ttaggacttg atcttacgga tgatagttta aaggggacac cgcgccgggt tgccaaaatg
    11941 tatgtgcagg aggtttttca gggtctcaac ccaaaaaaca aaccgtcggt aacgcttttt
    12001 aaaaaccagt atcattataa tcacatgctg gtagaaaaaa acataacttt atttacgtac
    12061 tgcgaacatc attttgttcc gattttcgga aaggtccatg tgggttatat tccggcgagc
    12121 caagtgatcg gtttatccaa aataaatcgt attgtgcaat actttgccaa gcgacctcaa
    12181 gtgcaagaac gtttgaccgt acagattgct gaggagttgc ggcgcattct aaatacggaa
    12241 gacgtggccg tagttattga cgcggcacat ttatgtgtgt catcgcgggg tgtacaagat
    12301 accgaaagta cgacgactac atccagtttt ttaggccgtt tccatgatga aggcgtgcga
    12361 aatgaatttt tatgttatat cgaacttaaa taaaaaacgt ttaacacatc tagactaata
    12421 tgaaacaaat aattttttta attgtatcca ttaacgtgct gttgtttggc atagcatgcc
    12481 gtgatgtacg tataaaaccg gagcaaccaa atatgaaatc tacgcagtcc gtgtacaaca
    12541 tcgctgtcaa aacaattgac ggcgccgaaa caaatttggc tacgtatcgc ggaaaaaaaa
    12601 tactcctcgt taatgtggca tccgagtgtg gttatacgcc gcaatataaa gatttacaaa
    12661 aattacagga acagtttgga tcaaaggtcg tcgttcttgg ttttccgtcc aatgattttg
    12721 gcggacaaga acccggctcc aatgctgaaa taaagaattt ttgcgagcgc aattataaaa
    12781 tcacatttcc tatgtttgat aaaatagccg tggtcgggga aggacaacat ccgctctacc
    12841 aatggctcag tcaaaaaaac ctcaatgggt ggaatgaaaa ggcgccgagc tggaattttt
    12901 gtaaatattt aatcgacgaa aacggggaat tgatcggata ttataattct tccgttaatc
    12961 cgctgagtga tgaaatcatt aacaaattat aacaaaatca cacaatgcac gttttagagc
    13021 gcgaacaaac tttgtcgtgt acgttgttgg atgcttggca atttttgtct tcaccacaga
    13081 atctcaaatt gattacaccg ccgtatatgg gatttgaaat ccgctcggat caaaacggca
    13141 aacccatgca cgccggaatg attattacct atcgggtaag ccctttattt aacataccca
    13201 tgacctgggt aactgaaata acccatgtgc gtgaacctta ttattttgtg gacgaacagc
    13261 gcataggacc ttatacgctg tggcatcatc agcattttct tgaagcggtg gagggtgggg
    13321 ttcgtatgcg ggattgtgta cattacaagt tgccaatggg aattataggg aatcagatag
    13381 aaaaatggat tgtacgccgt cgccttgaat ccatatttga ttttcggtgg cgtaaattaa
    13441 atgaaatttt caacgctcca tcctaagccg tatcactgat tttatcgcac ttttacttac
    13501 gcttatactc gcctcataat ttttaccagt cctttatttg aattcagatc gtacagatcg
    13561 ttaaacgagc ctgaagtcac aacgtccaat caatgaaaag gcattttgcc ttgcttgttt
    13621 cctcacagtc tattatattt taaatcgtta taaaataatc ttttattgat ctacggagcg
    13681 tttcaaggcg tggccttatt gaagtcatct tc
//
LOCUS       DAOQNQ010000056        13543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1608_length_13543_cov_5.103722, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000056 DAOQNQ010000000
VERSION     DAOQNQ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13543
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1608_length_13543_cov_5.103722"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..242
                     /locus_tag="PK754_05495"
     tRNA            161..242
                     /locus_tag="PK754_05495"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:195..197,aa:Leu,seq:gag)
     gene            246..1130
                     /locus_tag="PK754_05500"
     CDS             246..1130
                     /locus_tag="PK754_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB08990.1"
                     /translation="MNPNLFKGDSMTKSAPFLFYIILLFSTCPIAFGQEQDDNDDDYT
                     VQLENWKQKRKETDSLNQSRRVAREQARGYYKEGNRLSKAKRWNEAISIYHKAITADS
                     SYQKAYVNLGLAYRRTGEISRAIAYYDLAIHLPNANTNITAKAVQYKVWLLSDVKNYS
                     LAIETCNDFLKNNAVHPEISYIKAKLLKDQLNRTDEAVAILEQIVSHHPVHAASLYEL
                     SNCYNEAKEPERALRLINQAGNIEEEKWQYGIFFERAEALRLLNRIEEALADYRKAAN
                     DKRWKESAEYWIGVLQPK"
     gene            1144..1446
                     /locus_tag="PK754_05505"
     CDS             1144..1446
                     /locus_tag="PK754_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08991.1"
                     /translation="MSELIEGWAVVKTVSYEHQAELISDQLQSAGIENTVFSQKDSVN
                     TVWVGDLSEIKILVPHDKLQDALDVCKDIELPDMELSEDADVGESEEDEDRDENEE"
     gene            1452..2297
                     /locus_tag="PK754_05510"
     CDS             1452..2297
                     /locus_tag="PK754_05510"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HNB08992.1"
                     /translation="MQKIKTCAVEYLNTRPIVYGLQQAAAARFELQYAIPSVCADKLK
                     NKEVDLALIPSIEYSRIRGHRNLRIVPDIGVISHQFVKSVELFFNRNLENINTVAVDI
                     SSRTSVGLLDIILREKFDIHPEFIPMPPQVEVMLSKADAALIIGDNALELYDTWDNKI
                     DLGEEWGDMTDGLPFVYSFWAAAEDALNTDDILTLTASRDAGLDHITEISRVYSQERA
                     RGFEPDFYANYLTENIQFKLGKAELEGLKEYYSYCFYYGIIDEIPDLLFVGQENESDF
                     TVKNN"
     gene            2349..2933
                     /locus_tag="PK754_05515"
     CDS             2349..2933
                     /locus_tag="PK754_05515"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNB08993.1"
                     /translation="MELQVEIKWIIEDEQSNGYILQLVETGPEKGRMLPVVIGAPEAH
                     SIALELQGIKPFRPFTHDLAVQILDALDAEVDKVVITELNKNTFFASIHIRSNSVGEQ
                     VIDSRPSDAIAIAIRVGSPIFVEEKVMEAAGLDVDVQSGAYVKQTKKVPASEASELSM
                     TPLEKLEKDLQKAVESENYELAAKLRDQINLLKS"
     gene            2951..4573
                     /locus_tag="PK754_05520"
     CDS             2951..4573
                     /locus_tag="PK754_05520"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNB08994.1"
                     /translation="MFIVSRYVIREHIGPFFFALALITSMLLLNFVLQAMRYIIGKGI
                     SMTVILEFIAYNLAGIIVLVVPMSILVATIMAFGRLASDNEVTALKAGGINFFKLIFP
                     VILVACIVTYGVFWFNDEVLPLANHNARVLKKNIQAKRPTLSIEPGVFLEGIENFSMI
                     VENKDELGSTIYGVTIFDKSDRNAQRTITAKRGSIAIEESNDNMILELEEGEIHEYHQ
                     GKNDQYQRIIFQKYKVIVAVENLSLKTSDESFRNDREKNIDQLMEEVARHRKERERQM
                     GLVLKNIEKKPESVTQLDPRARKLVEKYCVEFSWLDAINDYFMDWNLRLALDTAYVPY
                     TDYVLSDSLKQTIQTYLKDTLKHTLPVAESQPSALRSLDPSLGRRPNMSKSAIPVDSV
                     EMPLTAISNQVSGNISTVSSYQRLMDQSMVEVNKKYSIPMACLIFVLLGAPIGVKAKR
                     GNLGLAAGISLFFFIAYYFCLILGEDLADRGLMNPFFAMWFMNFFLGMLGCVLIYQTV
                     TEKTMGWLTIRSFFHSLKLTMKSSFKRKINEN"
     gene            4563..5315
                     /gene="rlmB"
                     /locus_tag="PK754_05525"
     CDS             4563..5315
                     /gene="rlmB"
                     /locus_tag="PK754_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNB08995.1"
                     /translation="MKTNIIFGRQPVFEALKSGIPVKEVILQIGAHGDAIDEILNVCK
                     RKQIRVRTMDARYFSDMIGGKNNHQGAMVLLDQKEFQYVEMDVIFERAAVAGEKPLVA
                     ILDEITDPHNLGAIIRSAECAGFHGVVIPKHRSAEVNATVVKTSAGATAYIPVVQVTN
                     LTQALEELQERGVWIYGTSLDGDRDYDAIDVRDAVGIVIGSEGKGMRKMVAEHCDFLI
                     RIPMSGQIQSLNASVAAGIVFFDVMRQRRKGR"
     gene            complement(5398..6219)
                     /locus_tag="PK754_05530"
     CDS             complement(5398..6219)
                     /locus_tag="PK754_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08996.1"
                     /translation="MIERSNFSKDKSIDLDQPESIIDWFWVFWGCVIGIVIIGAFTFI
                     TSTKYNPDVVKAVPMLIGGLAFAVTGMILGHYSPGHTVKEAAVAGLVIPIIGFVLSGM
                     GYGPTFIAELSIFEKIGVALAGMLICQLGGWVGEELEGYDHPTKWLQWHWIIVAVVIG
                     FMLNSFVIFFVGLISLTPVPVFMALSVFAAGIVAGYKSPGHTEKECSIAGALTILLDY
                     LFITFALGIEAKDFGIESGTMAIIVLVALAVAGGLGLLGGWIGERMQIQEKELNN"
     gene            complement(6318..8714)
                     /locus_tag="PK754_05535"
     CDS             complement(6318..8714)
                     /locus_tag="PK754_05535"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNB08997.1"
                     /translation="MAKGDFFKNLRANTGALICLIISLLVFIPGLVLKVLPDLSDNLI
                     AFYLLNIGLTFASLLASVIGIYLAGFQNKTLSGIFITLLAVWLGSFLVNVKAIESVEL
                     KTLDQRFSFRYQYIDQEKRLTPEESPIIIVDVSDQAGEEVRDRYPWPRTYYAHFIRNM
                     KRAGAKVVGIDIVFDAPDYNGPEKDDDLAAAIKECGNVVLAGKVIKGDVASGGGSIGT
                     TAKPLPKFLDEGIGKWGFVGVTNDIDGFWRQYLVEEKTGDDEHEQVFHSFGVEVLRKY
                     YDIPDSSKFINNEQFFQFGDKVIRKYKPYSFLINFAGPKATFPYKSFELTVDDAAFDL
                     KEGRDLDSFDDEGDPDLGIPPGLLKSGFFKDKIVLLGATMEELHDVFSVPISEVRTSD
                     GKKLETLMPGVEIHANAIYTIIKDNYVTIQADWIRVLIVTLSAFVVFLLVSRLNKPLL
                     ALPILLLIIASVIALAFYLFAKHNIHMQLVTPVLAIMFTYVGNVIFQYLGERKEKAQI
                     RNAFGRYLPEKVVAQLVENPGLLKLGGEERVLSILFSDVAGFTTISEKLTPVELVHLL
                     NEYLTAMTNIISKYDGIIDKYEGDAIMAEFGAPLQDDEHAAKACYAALEMQEKLVEMR
                     VKWKREGRPELKARAGINSGNVVLGNMGSESVFDYTVMGDNVNLASRLEGANKEYGTY
                     IMISEMTLQFVKEQVITRELDILRVKGKAKGVRVFEVIARTSTGITANMKKTIENYNK
                     GLELYRQQRWDEAMVLFKTALTTNPDDGPSQTYIERCEEFKLNPPPENWDGIFEMKTK
                     "
     gene            complement(8798..11491)
                     /locus_tag="PK754_05540"
     CDS             complement(8798..11491)
                     /locus_tag="PK754_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB08998.1"
                     /translation="MQLIPNSSRRGLMAALFFFASFVLQSNAFAGGEILHTPLTSLPE
                     GQPVYFESAFAVPVEHAYLNYRRPGQANFTNFEMSQKVNGSFSAYIRELNGLKNGDQI
                     EYYISAETRSGQTITLPTENPMYMPFQMIISKRSVFQETGIEVVVLSPEPDEKTNKKD
                     VVIAISLFSDNAIDVKNLRLKLDGDDITAKADVTPELITYSPKDLGVGQHVARLMYIS
                     GESETQILTEFTFEVVLSGGEDILASKGFRAASDLTSGGSETAFEDGTFRLAGRAEHR
                     AQQNLGETTVFERVGGDISYEKKWFQVGATFDWDSEDNPKKNQPISRYLVTANVDNLV
                     ILNYGDTYPTFSPVTLNGTRVRGFSGGVYLGVFNFEYVTGEVNRKVLSDQNKKNKSRI
                     DSIFHNTDTSVTSYADAYQAYFESNPNGYFSGDFKRNMTAGRFSVGPQSFQFGVSFVK
                     TKDQVSSLLMNPITAVGYNGLTPQENLVAGADFRASMFNKRLNFDASVASAISNTDIT
                     SGSISHADLDSADIDLTKSQYDLVKKFITLNANLDPLITEFDKNLLAYTVGSSISAYN
                     NNLSVRYRFNGAFFQSYGASVARDQESFEIVDRMRFWQNRIFLTINYATMKNNLAKTV
                     ANTPKTNTLGFNASFFLPNKLPSVTLGYQTMNRDNGFEYTGSATSANSKYKDQIRANA
                     RPEDNTTNTLSFNTTYGFTFSELRNSASFSLSNSTRKDNSKDIGTAFLFETEASVIAE
                     DTIVDYYKAAAGSKSNTIGLSLSTDWKFPLRTSIGFSNTSGETKEIKKSDDNKRDSLT
                     ITKKTGAFSLNANGDYLVFQNDDVRINTTAGYNFASVTIPNVPDVTLQTISLGSRINF
                     FEKNNVTLSYSITSGLKIPTASGGSKSVTNSLFLARYEYIF"
     gene            11814..>13543
                     /locus_tag="PK754_05545"
     CDS             11814..>13543
                     /locus_tag="PK754_05545"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNB08999.1"
                     /translation="MHRMLSFFFKSVFIIIVSCTFPLSAQYEDNSHTMAFSRTMHYVL
                     SKNDKGSYQLKSEIKTKIRYLSERALKNDTFYVTEEFSAKVNKLNGSYIKKEGFGTPQ
                     IGFYYAKPEDVFIGNYKIHSITFDKPLKVKDSIQYSYQETFTEAAYMPLIEVPNVDYV
                     EYLQINIDHPRDTYVDFEIHYTADTLDCRIDRQSEKTTLTFRNINYLTPLAHNPFERI
                     RAVVLTKLQNAKREPINPYTPASFTSWYNGKLAQNGLTDDRHDSLAQAITRGLEEPMD
                     KLRAIHNHVKNRIRYIAVADSFHAIIPHRSKKVLELNYGDCKDRALLLMLLARSAGIP
                     VRMALVSTTPRLRFDGVHTSFFNHVINVYEADGKRFYFDPTAKYCEFGNLPPTDFNGR
                     VLILNEADPVYTDEPPLPRKLSIEVEVNGEMDSLRAAKASIKLRNEYLSSARHARYEL
                     SGQTLENYLSRMINSNFSKISMDQFHFENETDSTMTFNAKADLSEFIIQTATRKYIPQ
                     APFQSVSSQTGERIKDTWPVYMKNPELLGMILRLKIGDAKVNYKDIALGDKGVAWFSN
                     RTSLDASRGM"
BASE COUNT         3969 a         2794 c         3001 g         3779 t
ORIGIN      
        1 attatagaaa aagaactgga acaaggtggc ggacagtcta cgcctgcgct tcctacaccg
       61 caaaactaag aaccgcaaaa cattcgtaag atttacgggt tttgcagctt tttttattgc
      121 aaaaaaataa aattagcgta tatttcgtgc gctaatttat gcggaagtgg cgaaattggc
      181 agacgcacca tcttgagggg gtggcgccgg taggcgtgcg ggttcaaatc ccgccttccg
      241 cacttatgaa tccaaattta tttaaaggcg attcgatgac aaagtcggcg ccttttttgt
      301 tttatatcat tttgttattt agtacgtgtc ccatcgcttt tgggcaagaa caggatgaca
      361 atgacgatga ttatacagtg caacttgaaa attggaaaca aaagaggaaa gaaaccgata
      421 gtctcaatca atcccgtcgc gtagcgcgag aacaggcgcg gggttattac aaagagggga
      481 atagattaag taaagctaaa cgctggaacg aagcgatatc catttatcat aaagcgatta
      541 ccgccgactc gtcttaccaa aaagcttacg taaatctcgg cttggcttac cggcgtaccg
      601 gtgaaatcag ccgcgctata gcgtattatg atttagccat tcatcttccg aatgcaaata
      661 caaatataac cgcaaaggct gttcaataca aagtatggct tctctcggat gttaaaaatt
      721 attcgctagc tattgaaact tgtaatgatt ttttgaaaaa taatgcggtt catcctgaaa
      781 tttcttacat taaagcgaag cttctaaagg atcaactgaa tcgaacggat gaagccgtgg
      841 caattttaga acagatagtt agtcatcatc cagtgcatgc cgcttctttg tatgaactat
      901 ctaattgtta caatgaagcc aaagaaccgg agcgagcttt acgattgata aaccaagccg
      961 gaaatataga agaagaaaaa tggcagtacg gaattttttt tgaacgagct gaagctttac
     1021 gacttttgaa tagaatcgag gaagcattag cagattatcg gaaagcggcc aacgataaac
     1081 gatggaaaga aagcgcggaa tactggatcg gagtactgca acccaaataa aacggaggtt
     1141 gttatgagtg aattaatcga aggttgggcc gttgttaaaa cggtttccta tgagcatcaa
     1201 gcggagctga tcagtgacca gctacagtcg gccggtatag aaaataccgt gttcagtcaa
     1261 aaagacagtg tcaatacggt gtgggtagga gacttatcgg agatcaaaat cctcgttcct
     1321 catgataaat tgcaggacgc tttggatgtt tgtaaagaca tcgaattacc tgatatggag
     1381 ctgtctgagg acgccgatgt cggtgagtca gaagaagatg aagatcgtga tgaaaatgag
     1441 gaataagacg tttgcaaaaa attaaaacct gtgctgtaga gtatctaaat accagaccta
     1501 tcgtttatgg tttgcagcaa gccgcggcag cgcggtttga acttcaatac gctattcctt
     1561 cggtctgtgc ggataaactt aaaaacaaag aagtcgatct cgcattgata ccgtcaattg
     1621 aatattcgcg tattcgcggc catcgaaacc ttcgaatcgt gcccgatatc ggtgtgattt
     1681 ctcatcaatt tgtgaaaagt gtggagcttt tttttaatcg aaatcttgaa aatattaata
     1741 ccgttgccgt cgatattagc agccgtacat cagtggggtt gctggatatc attctgcgtg
     1801 aaaaatttga tattcaccct gaatttattc ctatgccgcc gcaagtcgaa gttatgctgt
     1861 caaaggccga tgcggccttg atcatcggtg ataatgcgct tgagttatac gatacatggg
     1921 ataacaaaat tgatctggga gaagagtggg gtgatatgac ggacggatta ccatttgtgt
     1981 attcgttttg ggccgccgct gaagacgcat tgaatacgga tgatattctt acactgacag
     2041 cttcccgcga tgcgggcctg gatcacatta cggagatttc acgagtttac agtcaggaac
     2101 gcgcgcgagg ttttgaacct gatttttatg ccaattatct cacggagaat atacaattca
     2161 aacttgggaa agcggaactt gaagggctga aggaatatta ttcttattgc ttttattacg
     2221 gtatcatcga tgaaataccc gatctgctgt ttgtcgggca ggaaaacgaa tccgatttca
     2281 ccgttaaaaa taattgattt tccttaagtc gtatattaac ttattctaga acagaaacgg
     2341 aaattgtaat ggaattgcag gtcgaaataa aatggattat tgaggatgag cagagtaatg
     2401 gctatatcct gcaattggta gaaaccggcc ctgaaaaagg tcgcatgtta ccggttgtta
     2461 tcggcgctcc ggaagcgcat tcgatcgcac ttgaacttca gggtatcaaa ccttttcgcc
     2521 cgtttactca tgatttagcc gtccagattt tagacgcctt ggacgctgaa gttgacaaag
     2581 ttgtcattac cgaattaaat aaaaatactt tttttgcttc gattcatatc cgcagtaatt
     2641 ctgtcgggga acaagtgatt gattcgcgcc cgtcggacgc gatcgcgata gcgatacgcg
     2701 tcggctctcc gatttttgtg gaagaaaaag tgatggaagc tgcgggattg gatgtggatg
     2761 ttcaatccgg cgcgtatgtc aaacaaacaa aaaaagttcc ggcttcggaa gcatccgaac
     2821 tttccatgac accgcttgaa aaactggaaa aagaccttca aaaggccgtt gagtctgaaa
     2881 actatgagtt ggccgccaaa ctccgcgatc agataaatct cctcaaatct taataccgaa
     2941 acgtcaccgc atgtttatcg tttcccgcta tgtgatccgc gaacacatcg gaccgttttt
     3001 ctttgcgctt gcgcttatca catccatgct tttactcaat tttgtactgc aagcgatgcg
     3061 ctatattatt ggcaaaggca tcagcatgac ggtgatttta gagttcattg catacaatct
     3121 tgcgggtatc atcgtattgg tcgtcccgat gtctattctc gtcgcaacga tcatggcgtt
     3181 cggtcgcctc gcctccgaca atgaagtgac ggcccttaag gccgggggta ttaatttctt
     3241 taaattgata tttcctgtca ttctcgttgc gtgtattgtt acctacggtg ttttttggtt
     3301 taatgatgaa gtgctgccgt tggctaatca caatgcacgc gttttaaaaa aaaatatcca
     3361 ggctaagaga ccgacgcttt ctattgaacc gggtgtgttt ttggagggca ttgaaaattt
     3421 tagcatgatc gttgaaaata aagatgaact cggatcgaca atttatggcg tgacgatatt
     3481 tgataaatcc gaccgtaatg cacaacgaac cataacggcc aagcgcggat ctatagcgat
     3541 cgaagaatcc aacgataata tgatattgga gttggaagaa ggtgaaatac acgaatacca
     3601 tcagggcaaa aatgatcaat atcaacgcat aatttttcaa aaatacaaag ttatcgttgc
     3661 ggttgaaaac ctatcgctaa aaacaagtga tgagtccttt agaaacgatc gcgaaaaaaa
     3721 tattgatcag ctcatggaag aagtagcacg gcatcgcaaa gaacgtgaaa ggcagatggg
     3781 gttggtttta aaaaatattg aaaaaaaacc ggaatccgtt acgcaattgg atccccgcgc
     3841 acgcaaatta gtcgaaaaat attgtgtgga attcagttgg cttgatgcga tcaatgatta
     3901 tttcatggat tggaatctac gtttggcgtt ggatacggct tatgtcccat atacggatta
     3961 tgttttatca gattctctga aacaaactat acaaacttat ttgaaagaca ctttgaaaca
     4021 tacattaccg gtcgctgaat cacaaccatc ggctttgcgc agtttagatc cgagtttggg
     4081 gcgaagaccc aatatgagca agtctgcaat accggtagat tctgtagaaa tgccgttaac
     4141 cgcgatcagc aatcaggttt cgggaaacat cagtaccgtt tcgagctatc agcgactgat
     4201 ggatcagagt atggttgagg taaataaaaa atattcgata ccgatggcat gtttgatttt
     4261 tgtgttattg ggtgcgccta tcggtgtgaa agccaagcgt ggtaatttgg gtctcgccgc
     4321 agggattagt ttgtttttct ttattgcata ttatttctgt ttaatactcg gagaggatct
     4381 ggcggatcgc ggactaatga atcctttttt tgcaatgtgg tttatgaatt tctttttagg
     4441 catgcttggg tgtgttttga tatatcaaac cgtaactgaa aaaacgatgg gttggttgac
     4501 catccgatct ttttttcatt cgttgaagtt aacaatgaag agtagtttta aacggaaaat
     4561 taatgaaaac taatattatt ttcgggcgac aacctgtttt cgaagcgctt aaatctggaa
     4621 ttcctgtaaa agaagtaata ctgcaaatcg gcgctcatgg cgacgccatt gatgaaattc
     4681 ttaacgtctg caaaagaaaa caaattcgtg ttcgaaccat ggatgcgcgg tatttttcag
     4741 atatgatcgg cggaaaaaat aatcaccaag gcgccatggt attgcttgat caaaaagaat
     4801 tccaatatgt ggaaatggac gttatatttg aacgtgccgc cgtcgccgga gagaaaccgc
     4861 tcgttgctat tttggatgaa ataaccgacc cgcataatct cggagcgatt attcgcagcg
     4921 ccgaatgcgc cggctttcac ggtgtagtca ttcctaaaca tcgttcggca gaagttaacg
     4981 caacagttgt aaaaacatct gcgggcgcaa ccgcgtatat tcccgtcgta caagtaacta
     5041 atcttacaca agcgcttgaa gaattacagg aacgcggggt ttggatttac gggacaagtc
     5101 ttgacggaga tcgcgactat gatgcgattg acgtacgtga tgctgtcggt attgttatag
     5161 gtagtgaagg taaagggatg cgtaaaatgg tagccgaaca ctgtgatttt ttgattcgca
     5221 tcccgatgag cggccaaatc caatcgctca atgcctcggt ggctgcgggc attgtctttt
     5281 ttgatgtcat gcggcaaaga cgaaaaggac gataatgtag aactaaagca tgcatataaa
     5341 atgaaaaaac cgaatgctgt cattcggttt tttcatttgg aactataatt ttaggtatca
     5401 gttgtttaat tctttttctt gaatctgcat acgttcgccg atccatccgc ccaaaaggcc
     5461 taacccgccg gcaacagcga gagcaactaa tacgatgatg gccatcgtgc cggattcgat
     5521 accaaaatct ttagcttcga tgcccaatgc aaaagtaata aacaagtaat ccaatagaat
     5581 cgttaaagca cccgcaatac tgcattcctt ttccgtatgt cctggtgact tataacctgc
     5641 tacaattccg gctgcaaata cgctcaacgc cataaaaaca ggtaccggcg taagggaaat
     5701 caaaccgacg aagaaaatca caaaactatt gagcataaag ccgatcacaa cggcaacgat
     5761 aatccaatgc cattgtagcc atttcgtggg atggtcataa ccttccaatt cttcaccgac
     5821 ccaaccgccg agttgacaaa tcagcatacc ggccagtgcg acaccgattt tttcaaatat
     5881 gctgagttcg gcaataaatg tggggccgta tcccatacct gagagcacaa agccgatgat
     5941 gggaataact aatccggcta cagcagcttc tttgactgta tgaccgggag agtaatgacc
     6001 taaaatcata ccggtaacgg cgaaagcaag tccgccgatc aacatcggta cggcttttac
     6061 aacgtccgga ttgtatttag tagatgtgat aaacgtgaaa gcgccgatga taacaatacc
     6121 tattacgcaa ccccagaaaa cccaaaacca atcaatgata ctttcgggtt gatcaagatc
     6181 aatagacttg tcctttgaaa aattgctccg ttcgatcatg gataactcct catagtgcga
     6241 tatatgaatt tagaaataga aatttcagat tgggcttttg gacgccccat aataatcaat
     6301 ctaaaaacta ttgtcaatta tttcgtcttc atttcaaaaa taccgtccca attttccgga
     6361 ggcggattga gtttgaactc ttcacatcgt tcgatatagg tctgcgaagg accgtcgtcc
     6421 ggattagtag tgagtgccgt tttgaaaagc accatagctt catcccaacg ctgttgacga
     6481 tacagttcga ggcctttatt atagttttcg atagtttttt tcatatttgc cgtgataccc
     6541 gttgatgtgc gggcgataac ttcaaatact ctgacgcctt tggctttacc ttttacgcgc
     6601 aggatatcaa gttcgcgggt aataacctgt tctttgacga actgaagtgt catctcgctg
     6661 atcatgatgt acgtaccata ttctttattt gcgccttcga gacgagaggc aaggttcaca
     6721 ttatcaccca taaccgtata atcaaaaacg gattcggaac ccatattacc gagaacgacg
     6781 ttcccggaat tgatgccggc acgcgcttta agttcagggc ggccttcacg tttccatttg
     6841 acgcgcattt cgacgagttt ctcctgcatt tctaacgccg cataacatgc tttggcggcg
     6901 tgttcatcgt cttgcaaagg tgcgccaaac tccgccatga tcgcatcgcc ttcatattta
     6961 tcaatgatgc cgtcgtattt ggaaataata ttggtcattg cagtcaggta ttcattaagc
     7021 aagtgtacca actcaaccgg agtcaatttt tcagaaatcg tcgtaaaacc cgccacgtcg
     7081 gagaaaagga tggaaagaac acgttcttca ccgccgagct tgagcaggcc cggattctct
     7141 acaagctgcg caactacttt ttcaggcaag taacgaccga atgcattacg aatctgtgct
     7201 ttttctttac gctcgccgag atattgaaag atcacattac cgacataggt aaacatgatc
     7261 gccaataccg gcgtcacgag ttgcatgtga atgttatgtt ttgcaaataa ataaaatgct
     7321 aaggcgataa ccgatgcaat gatcagtaat aaaatcggca atgccagtaa cggtttatta
     7381 aggcgactga ccaagagaaa cacaacaaat gcgctcaacg ttacaattaa aacacgaatc
     7441 caatccgcct gtatggtaac atagttatct ttgataatgg tataaattgc gttggcgtgg
     7501 atttcaactc cgggcatcag ggtttctagt ttttttccat cggaagtgcg cacttcactg
     7561 atcggtacgg aaaacacgtc gtgcaactct tccatcgtcg caccgagaag aacgattttg
     7621 tccttaaaga aaccggattt aagcagtccg ggggggatcc ctagatcggg atcgccttca
     7681 tcatcaaacg aatccagatc gcgcccttct ttgagatcaa atgctgcatc gtccaccgta
     7741 agttcgaacg atttataggg gaatgtagct ttagggcctg cgaagttaat caaaaaagag
     7801 tagggtttat acttacgtat aactttgtcg ccgaactgaa aaaattgttc attgttgatg
     7861 aattttgaag aatcagggat atcataatat ttgcgtaaaa cttcgacgcc aaatgagtga
     7921 aaaacctgct cgtgttcatc atcgcccgtt ttttcttcga ccagatattg cctccaaaaa
     7981 ccgtcaatat cattggttac accgacaaaa ccccattttc cgatcccttc atccagaaat
     8041 ttaggaagcg gtttggctgt tgtgccgatg cttccaccgc cggaagctac atcaccttta
     8101 atgactttgc cggccaacac gacattacca cattctttga tggccgcagc aagatcatca
     8161 tctttttcgg gtccattata atccggggca tcaaacacaa tatctattcc gaccacttta
     8221 gcgccggcgc gtttcatatt gcggataaaa tgggcgtaat acgtgcgtgg ccaaggatag
     8281 cgatcacgaa cttcttcacc ggcttgatcg ctcacgtcaa cgatgatgat gggtgattct
     8341 tcaggagtta atcgtttttc ctgatctatg tactgatatc gaaaactaaa cctctgatcc
     8401 aatgttttca gttcgacgct ttcgatggcc tttacgttga cgagaaaact tccgagccaa
     8461 acggccagca gagttataaa aataccagat aatgttttat tctgaaaacc ggcaagatat
     8521 ataccgataa cggaagcaag caatgaggca aaagtgagcc cgatatttaa cagataaaat
     8581 gcgataagat tatcgctcaa gtcggggagt acttttaaaa ccaagcccgg aataaatacc
     8641 aataaactga tgatcaaaca aatcaatgca ccggtattgg ctcggaggtt tttgaaaaaa
     8701 tcaccttttg ccataatgcc tcgtcataaa gatagtagag tggataaaaa gagagcctga
     8761 tttgtaatca aatcaggctc tcgcggataa gatcgaatta gaaaatgtat tcgtatcgtg
     8821 ccaggaatag actgttggtt accgatttgg aaccaccgga agccgtagga attttgagtc
     8881 cggaggtaat gctgtaactc agcgtcacat tatttttttc aaagaaattg attcgtgaac
     8941 ccaaagaaat cgtttgtaga gttacatcgg gaacattggg tatcgttacc gatgcaaaat
     9001 tgtatccggc tgtcgtatta atacgcacat catcgttttg aaatacgaga tagtcgccat
     9061 tggcattaag actgaatgcg ccggtttttt ttgtaatcgt caaggaatcc cgtttattat
     9121 cgtctgattt tttaatttct ttagtttcac cgctcgtatt gctaaatccg atcgaagtac
     9181 gtaacgggaa cttccagtcc gtcgaaagac tcaagccgat ggtattgctc tttgatccgg
     9241 cagccgcttt gtaataatcc acaatcgtat cttcagcgat gactgaagct tcggtctcaa
     9301 agagaaatgc cgtacctatg tctttgctgt tgtctttacg tgtgctgttg gataagctaa
     9361 acgacgccga attacgcagt tcgctgaacg taaaaccata ggtagtgttg aaactgagtg
     9421 tattagtagt attgtcttcc gggcgcgcat ttgcacggat ttggtcctta tatttggagt
     9481 tagccgaagt agcagaaccg gtatactcga agccgttatc acggttcatg gtttgataac
     9541 ccaatgtcac cgatggtaat ttattgggca agaagaaaga cgcattaaat ccgagcgtgt
     9601 tagtttttgg tgtattggcg acggttttgg caaggttatt cttcatcgtc gcatagttga
     9661 ttgtaaggaa tatgcggttt tgccaaaacc gcatgcggtc gacgatttca aacgactctt
     9721 gatctctagc tactgatgcc ccgtatgatt ggaaaaacgc accgttaaag cgataacgca
     9781 cactcagatt gttattatat gcagaaattg aactaccaac agtatatgcg agtaagtttt
     9841 tatcgaattc agttattagt ggatcgagat tagcgtttag cgtaataaac ttctttacta
     9901 aatcgtattg acttttagtc aggtcaatat ctgctgagtc tagatcggca tgagaaattg
     9961 aaccggatgt aatatcggtg ttactgatag cgctggcgac cgatgcatca aagttcaaac
    10021 gcttattgaa catgcttgct cgaaaatcag caccggcaac caaattttct tgcggggtca
    10081 agccgttata gcccacagcc gttataggat tcatgaggag tgagctaact tgatctttgg
    10141 tttttacaaa acttactccg aattggaatg actgggggcc tacagagaag cgtccggcgg
    10201 tcatattacg tttgaaatcg cctgaaaaat aaccattggg gttgctttca aaataagcct
    10261 gataagcatc ggcgtaagag gtaacggaag tgtccgtatt gtggaaaata gaatctattc
    10321 tgcttttgtt ctttttattt tgatcactca atactttacg gttaacttca ccggtgacgt
    10381 actcaaaatt gaacacgcca agataaacgc ccccgctaaa accgcgtaca cgcgttccat
    10441 tgagcgtaac agggctgaaa gtggggtagg tatcgccgta gtttaaaatc acaagattat
    10501 caacattggc cgtcaccaag tatcgcgaaa tgggctgatt tttcttagga ttatcctctg
    10561 aatcccagtc gaaggtcgcg cccacttgga accacttctt ttcatacgat atgtcaccac
    10621 ccacgcgttc aaatacggtg gtttcgccaa gattctgctg tgcgcgatgt tccgcacgtc
    10681 cggctaaacg gaaagttccg tcttcaaaag cggtttcgga tccccccgag gttagatcgg
    10741 atgcggcacg gaagccttta gacgctaaaa tgtcttcacc gccacttagt accacttcaa
    10801 atgtaaattc ggttaagatt tgcgtttcgc tttcacctga aatatacatc aaacgtgcta
    10861 cgtgttgacc cacaccaagg tctttggggg aataggtgat cagttccggc gtaacatccg
    10921 ctttagccgt aatgtcgtcg ccgtctagct tgaggcgaag attttttacg tcaatagcat
    10981 tgtctgaaaa taaggaaata gctataacaa cgtctttttt attggtcttt tcatcgggtt
    11041 ccggtgataa aacgaccact tcaataccgg tttcctgaaa aaccgaacgt ttgctgatga
    11101 tcatttgaaa cggcatatac atcggatttt cagtcggcaa agtaattgtc tgccccgaac
    11161 gcgtttcggc ggaaatgtag tactcgattt ggtcgccgtt ttttaatccg ttgagctcac
    11221 gaatatacgc gctgaaacta ccattgactt tttggctcat ttcaaagttg gtaaaattag
    11281 cttgacccgg acggcgatag tttaagtaag cgtgttctac cggcacagca aacgcagatt
    11341 caaaatatac cggttgacct tcgggaagac tcgttagcgg cgtatggaga atctcaccgc
    11401 cggcaaatgc attgctttga agcacaaaac ttgcgaaaaa aaacagcgca gccataaggc
    11461 ctcgccgact cgaattgggg atcaactgca taattcctcc taaatttaga accgcattca
    11521 ataaaacagt caaagttgat tttaattaaa agacagtggt gcagatgact ttacaccaaa
    11581 ttaattgcag acaggtcaga tcctatcctg ataaaacacg tggcattcgt aaaatgaatg
    11641 ctggttacca taataatcca atgaagagtt aacatctgta atgtaaagaa tttatgcgca
    11701 taagtgcaaa ctttttttac tggttggcat tgtatctaga tttgattttt attgaatttg
    11761 gcgcgatgct gttttattgt tatcccgttt aaacggattc aaaaaagggg aatatgcata
    11821 ggatgttatc attctttttc aagtcggttt tcatcattat cgtaagctgt actttcccat
    11881 tgtcggcaca gtatgaagac aattcgcata ctatggcgtt ttcacgtacg atgcattacg
    11941 tgctaagcaa aaatgacaag gggagctatc aattaaaatc tgaaattaaa actaaaattc
    12001 ggtatttgtc cgaacgcgcg ttgaagaacg acacgtttta cgtaaccgag gaattttcag
    12061 ccaaagtaaa caagctgaac ggttcgtata ttaaaaaaga aggttttggt accccgcaaa
    12121 ttggttttta ttatgcaaaa ccggaagacg tgtttatcgg caattacaaa atccacagta
    12181 taacatttga taaaccgtta aaggtcaaag attccataca atattcatat caggaaactt
    12241 tcactgaagc ggcatacatg cctttgatcg aggtgcccaa tgtggattat gtggaatatc
    12301 tacaaataaa tattgaccat ccgcgtgata cgtatgttga ttttgaaata cactatacgg
    12361 cagatacact ggactgccgg attgatcgtc agagtgaaaa aacaacattg acgtttagaa
    12421 atatcaacta tcttacaccc ttggcacata acccgtttga acgcattcgt gccgtagttt
    12481 tgaccaaact gcaaaacgca aaacgcgaac cgatcaatcc ctacacaccg gcttcattta
    12541 catcgtggta caacggaaag ctcgctcaaa acggtttgac cgatgatcgt catgactctc
    12601 tggcgcaggc tatcacgcgg ggattagaag agcctatgga taaactcaga gcaatacata
    12661 atcatgtcaa aaatcgtatt cgatacatcg ctgtagcgga ttcctttcat gcgatcatac
    12721 cgcatcgctc caaaaaagta ctcgaattaa actacggtga ttgtaaagac cgggcattgt
    12781 tgctgatgct tttggcgcgt tctgcaggta tacctgttcg tatggcgctc gtttcgacca
    12841 cgcccagact tcgttttgat ggcgtccata catcgttttt taatcatgtg atcaatgtct
    12901 atgaagccga cgggaaacgt ttttattttg atcctacggc taaatattgt gaatttggga
    12961 atcttcctcc gacggatttc aacggacgag tattgattct caacgaggcg gatccggttt
    13021 ataccgatga gccgcctttg ccgcgaaaat tatccatcga agtggaagta aacggagaaa
    13081 tggacagttt gcgcgccgcg aaagccagca tcaagcttcg taatgaatat ttatcgtcgg
    13141 cccgtcatgc tcggtatgaa ctttccggac agacgcttga aaattattta tcccgaatga
    13201 tcaactccaa tttcagtaaa ataagcatgg atcaattcca ttttgagaac gagacagact
    13261 caaccatgac atttaacgct aaagcggatt tgtcggagtt tattattcaa actgcaacac
    13321 gcaaatatat cccgcaagct ccgtttcaat ccgtttcatc ccaaacgggt gaacgaataa
    13381 aagacacgtg gccggtgtac atgaaaaacc cggaattgct cggtatgata ctgcgattga
    13441 aaataggcga tgcaaaggtg aattataaag atatcgcgct cggcgacaaa ggggttgctt
    13501 ggtttagtaa tcgaacttca ttggatgcgt cgcgggggat gtt
//
LOCUS       DAOQNQ010000057        13542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1609_length_13542_cov_6.736635, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000057 DAOQNQ010000000
VERSION     DAOQNQ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13542
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1609_length_13542_cov_6.736635"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2966
                     /locus_tag="PK754_05550"
     CDS             <1..2966
                     /locus_tag="PK754_05550"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNB09000.1"
                     /translation="MDPSNSNIVWVGTGESNGQSNVIFGDGIYRSEDGGKSWKNMGLE
                     NSEHIGGIVIDPRNSNTVYAAAYGPFRNDGGERGVYKTTDGGKTWTRVLHVSDYTGFW
                     QIHMDPNNSSVLYAVAHQRFKKLYATVQGGPESAIYRSTDAGVSWQKVMSGLPSEDVG
                     KIGLAIAPSNPQVLYAIVQAKEKGGVYRSSDRGASWVKRSSYVSTYAFYFQELFCDPK
                     DENTVYSMDVFNQISRDGGATWSKLGEKYKHVDNHALWINPINTKHLIAGSDGGVYES
                     LDQGKTWDFKANMPIAEVYKVSIDQALPFYHVYAGTQDNNSFFGPSRTNSSAGVTNQD
                     WTFTLGGDGFETQADWSDENILYVQSQNGGLVRYDKKTGEQLFIKPIDTADVGYRFDW
                     DAALLISHHDPKRLYFGGHRLFKTDDRGNTWTLLSGDLTRGVPQKLIKMQGKSWSYDE
                     LASKGSLANITAIAESPIDPNILFVGSGDGLIHYSDDGGKTWNKPASTGGLPEFARVH
                     TIVASSHNRRVAYAACHNFGDGDNKPYLLKTSDGGKTWTLLNGNLPKRGSTYSFAEDH
                     VDPNLLFVGTMFGLYVSNTGGQDWVAFNNGIPPGLITDMTIQKRENDLVVATFGRGMY
                     ILDDYTPLRHLSKETLQKDIAILPIKEASMFIPSNPFGFPGTGFQGAGFFATPNPETG
                     AVFTYYVKDDYKSLKKKRRDTEKEKQKKGEDIELPSYQTLRNEADEQEPFFVFTIKDE
                     LGQPVRRIKTSVQKGVNRIVWDFRTNVFTPISLAPFDNSIPWNDPDRGYMVVPGKYTV
                     SVSRYEAGQWTDLGVSQSFICKPLLTPSLPVADRNKLDAFNKKVAELIRAISSADAYR
                     ESMVEKIPLLKQAVIETAKLPEGTYAAVVAAEDKLKRIQRQMNGDGLRALYEGVPPTS
                     LRGRVEIITGNLWTTTSDATTTFQHLYDVAAAQFDGLLRALQETDAEIKRIEQQMDRY
                     GAPFTPGRLPSVIKN"
     gene            3102..4649
                     /locus_tag="PK754_05555"
     CDS             3102..4649
                     /locus_tag="PK754_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNB09001.1"
                     /translation="MLAQILSATTFGIDAFIIKVEAHLEGAVPGFITVGLPDNAVRES
                     KERVNAAIKNSGFTFPIKRITINLAPADLRKEGSGFDLPIAVGILAAHGQIESHRTEE
                     YVVVGELSLDGSLRPINGVLSLALRARREHIKGIIVPAGNAAEAAMAEGLEVIPVNAL
                     SDAVQFLNGQKHIAPHQVDMSALFDQAPAMPVDFRDVKGQEHAKRALEIAAAGGHNII
                     MIGPPGSGKTMLAKRIPTILPDLTPDEALETTKIHSVAGFLPQNTPLVTLRPFRSPHH
                     TISDAGLIGGGHIPKPGEVSLAHHGVLFLDELPEFKKNVLEVMRQPLEDGHVTISRAE
                     MSLTYPANFMLACAMNPCPCGFHTDPGNRCTCNPGMIQRYMAKISGPLLDRIDIHIDC
                     PAIKYKELSSEGHGETSSAIRERVKKARQIQLKRFESLRKKGQPVYCNADMESRAIRE
                     FCTIDARSQELMKLAITKLGLSARAYDRILKVARTIADCANAPAIRAEHVSEAIQYRS
                     LDRSYSG"
     gene            4715..5437
                     /locus_tag="PK754_05560"
     CDS             4715..5437
                     /locus_tag="PK754_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HNB09002.1"
                     /translation="MSDAFLRAQWKNLIMANYAVDPSLLRPYVPSGTELDSWKDTFYV
                     SLVGFLFEDTRVHGLSFPFHRTFEEVNLRFYVRRFESGVWRRGVVFIKEIVPRSLITC
                     IANTVYHEHYATHAMRHRFTSDAHCKTFEYSFKSANGWNTISAKTNTESIAMAEGSEQ
                     EFIAEHYWGYTRINEKKLGAYEVRHPRWDIYPVMDYHIACDIGGLYGEPFAKTMARNP
                     DSVFVAKGSPIEVMKKTVIEHI"
     gene            5633..6541
                     /locus_tag="PK754_05565"
     CDS             5633..6541
                     /locus_tag="PK754_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09003.1"
                     /translation="MKASFKKILTVLVFSLIWSGCYTAVRQSGGYYSEFDHADAKQPE
                     ITQIQPSDEDSSDQDEEVTPSYDEDRDIYIQNYYGPEYPYPYGYWTPGWVNYRHPYYY
                     GAGMYFGPRVRLAYVGTYWGYGYYDPFYDPYYYYDGWCYYPTGYGHHDNNPIHPSQQR
                     TYGGRRVYTASSTPNLPGIVGVSVASSGGGTTQRIYRSGNRTSGESRIDQDRVRRNYR
                     KYYGFETGDKAVNAEAGRKSRNANRETSTRNKETRETRQVRDNNSRNNGGERNARSGE
                     RNSGRSGRSEGRSGETRSSGKRSARN"
     gene            6545..7141
                     /locus_tag="PK754_05570"
     CDS             6545..7141
                     /locus_tag="PK754_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09004.1"
                     /translation="MKYVGLLITILVSLYGSACYTVVGISRSDDRIQNEATESEPDFA
                     AYEPYGVTPSTMWEYYYETPFPWWYVPNADEYDAFDDTTTATDPTETSSYSSSRRNYG
                     RRRAALNDASSGSSVSTGYASPVYSPGGTTPSFVPSYSTGSSASYSSPSTPVKTDSTV
                     QSGSGSSDSNSNSTTKTQDTNRKRGYGNRRTAKSGDKK"
     gene            7156..8487
                     /locus_tag="PK754_05575"
     CDS             7156..8487
                     /locus_tag="PK754_05575"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNB09005.1"
                     /translation="MKFLSYGISLFFYSFILLAQNENNAVRPFMNEFGPGARALGLGG
                     AYSAIAEDYTAVYWNPAGLAQIRKMEFYTAFAHRNNTNAITYQGTRTETSNGFTSMNA
                     LGFVFPVPTTRGSLVLAFGFHRVNSFDDYNRVTGSPMIGNISFDQDEKTTNTGSLNHW
                     SFAGALDLTRNVSVGATLNIVTGKNDLSVDFFQDDEIAMLDDSIATKEVSFAEKPDYS
                     GFNFKIGTLFRPAKNLRVALTVTAPTKITVSENYSESTTIKTDNNGYYSDSWYEPYKY
                     HITSPWKFEIGSSYKYKLALFTGSVEWVDWQQTRFSSNIFDADGKNIDDAINNALLND
                     YRTSVNYRIGTEMVVPQIGCKVMAGYAYQQSPYKNGLEIVNSDHRYISVGASFLLDEQ
                     VKIDAAYQYGWWKQATTDNLLAYGPSPDYYNRYTSEKITSKKLLLSLSYRF"
     gene            8491..9486
                     /locus_tag="PK754_05580"
     CDS             8491..9486
                     /locus_tag="PK754_05580"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNB09006.1"
                     /translation="MKIKNYRLIIHGGIISAFLLYSFTLSAQVRPLDQAILRPGSGVY
                     SDLAHIEGETIKDMVAALSADSMEGRATGTRGFDKAARWVKEFFVQHGIQPLPGGYFQ
                     DFTVSTSVLKERLHSNEWSSETASTKNIIGYIAGNDSLRRNEFIVLTAHLDHLGKEGD
                     SIYAGANDNASGVASVLQIARWIKESKTDLGRSVVFILFSGEEAGLLGSYHFIENPWI
                     DLNKIRLLINLDLVGSGRDGIMLQGSENYPDYATRIKKINDERFGFEMSTRPNSPNSD
                     HYYFNKIGLPAFFVYAYRGTMPYHAPGDTSEKIDEKVLENVTRWVAAMVVAFGNE"
     gene            complement(9649..10578)
                     /locus_tag="PK754_05585"
     CDS             complement(9649..10578)
                     /locus_tag="PK754_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HNB09007.1"
                     /translation="MKKLFLTLTLLAAFTVSATDNTAASGSVVAVKEFTRSFPVSPGG
                     QLTLTTDFGSIKIVGSSDDKVSIMARMRGSDDDLKDFEITAEKTGNDVTIRGNWMRKN
                     NFFGHNSNSMDVDYIVNVPSQYNVSLGTAGGNIVIQDVKGLIQGGTSGGNLDLTNING
                     NAELKTSGGNIRAKSTTGNLNMRTSGGNVSMSNINGDLDVSTSGGSIRIEDINGRTNA
                     ETSGGHIEVYVSGNNKGVNVETSGGHIALYLPKNIAATIDASTSGGEVEFDLPIMVNG
                     KMRENKVRGTINGGGELIRAHTSGGDIRILTRN"
     gene            complement(10733..11638)
                     /locus_tag="PK754_05590"
     CDS             complement(10733..11638)
                     /locus_tag="PK754_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HNB09008.1"
                     /translation="MEVKDIKTVGVLGCGLMGAGIAQVAAASGFKTIVREVNQDVIDK
                     GVGNIKKQLERNAAKGKITAEEKDKTIANLTGTTSLDDLKNCDFIIEAIIEDVKIKNE
                     TYAYLDKICPPHTIFASNTSSLTVTEMAAATNRPDRFCGMHFFNPVPVMKLVEIVKAV
                     RTSEETFNRAFELGKAFGKTPIATKDNSGFVVNFLLVPYLLGAIRAVQNNIASIEDID
                     KGMMLGCGYPMGPLTLLDFVGIDTTYRIAEIMFKEYGEPMYSCPPLLRKMFLAGFYGR
                     KNGKGFYDYSSGTPVVNKDVMNFVK"
     gene            complement(11666..12136)
                     /locus_tag="PK754_05595"
     CDS             complement(11666..12136)
                     /locus_tag="PK754_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNB09009.1"
                     /translation="MNFILSCGAHNVKQTASVEPSPIIQPISQQDHSEKAFDFTMLND
                     PWIIEPRTYEVTIQPYTEPAEVVVTSSGKETSVIGYRVQLISTTDYYEALEIRDSVSV
                     RLSGEIYLDYEPPYYKVRAGNFIQRDDAEMKKAEAKQAGYPDAWIVRTKIIQKK"
     gene            complement(12163..12822)
                     /locus_tag="PK754_05600"
     CDS             complement(12163..12822)
                     /locus_tag="PK754_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNB09010.1"
                     /translation="MIKNIHQILFDFDYTLADSSEGIITCVNFALEKVGAPPGQPDAI
                     RRMIGHSLENTFARFIDPSDTEKIQTCKSLFMEHADTGAMVRNTVMLDGVRETLEFLY
                     NELYTLGIVSTKRRSTIWETLIEKEMDDFFDIVIGYEDVTHLKPHPEGLIKAIEELAG
                     TPETTIYIGDSPLDIKAAKSANVFVVAVTSGMHSTDELATLNPDLIIPRLSDLKAHLA
                     A"
     gene            complement(12815..>13542)
                     /locus_tag="PK754_05605"
     CDS             complement(12815..>13542)
                     /locus_tag="PK754_05605"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB09011.1"
                     /translation="AAIEEMSKTSYEIAHNIGNTKEMAIHAQSSADKGSQVIDETVDG
                     MQKMLAMVQQSSNVVNSLSESSRKITKVVSIIDDITKQVNLIALNASIEATRAGEKGK
                     GFAVVADEIKKLAERTAHSTTEINDIISKIQVNVNEAVNLMGRSNEEAANGIRLSQEA
                     KSSLKEISDVFKNVTMMVSQVAVSSQEQSVTGDQITDNVKVINTATQQIAGGINEIAK
                     SAEYLNNLTTTLQKTVKRFKIGD"
BASE COUNT         3602 a         2986 c         3206 g         3748 t
ORIGIN      
        1 tgatggatcc gtccaattcc aatatcgtgt gggttggcac cggtgaaagc aatggacagt
       61 ccaatgtgat ttttggcgac ggtatttatc gcagcgaaga cggcggtaaa agttggaaaa
      121 atatgggact cgaaaattca gagcatatcg gcgggatcgt tattgacccg cgtaattcca
      181 ataccgtata tgcggcggca tatggcccgt ttcgtaacga cggtggtgag cgtggtgttt
      241 ataaaaccac cgacggcggt aaaacctgga cacgtgtact tcatgtgagc gactatacgg
      301 gattttggca aatccatatg gatccgaata attcgtcagt tttatatgcc gtggcgcatc
      361 aacggttcaa aaaactatac gctaccgttc aaggcgggcc ggagagtgcg atctatcgca
      421 gcacggacgc cggtgtgtcg tggcaaaaag tgatgtccgg actgccttcc gaagatgtgg
      481 gcaaaatcgg actggcgata gcgccttcca accctcaagt gctttatgct atcgtgcaag
      541 ccaaagaaaa aggcggtgtg taccgcagca gcgaccgcgg tgcgagctgg gtcaaacgca
      601 gcagttatgt ttcgacgtat gcgttttatt ttcaggaatt attttgcgat cccaaggatg
      661 agaataccgt ttacagcatg gatgttttta atcagatcag ccgtgatggc ggtgcgactt
      721 ggtccaaact tggcgagaaa tacaaacacg tagataatca tgcgctttgg attaatccta
      781 taaacaccaa acacctgatt gcaggaagtg acggcggagt gtacgaatca ttagatcaag
      841 gtaaaacctg ggattttaaa gccaatatgc ccattgccga agtgtataaa gtttcgatag
      901 atcaggcgct cccgttttat catgtctatg cgggtacgca ggacaataac agtttcttcg
      961 gaccatcaag aacgaattca tcggccggag tgaccaatca agattggacg tttactttag
     1021 gcggtgacgg atttgaaacc caagccgact ggagtgatga aaacatactt tatgtgcagt
     1081 cccaaaatgg cgggcttgta cgttatgata aaaaaacggg tgaacaactt tttatcaaac
     1141 ccattgatac ggccgatgtg ggttatcgtt ttgattggga tgcggcgtta ttgatttcac
     1201 atcacgatcc caaacgattg tatttcggcg gtcatcgttt atttaaaaca gacgatcgcg
     1261 gcaatacgtg gacactgctg agcggcgatc tgacgcgcgg tgttccgcaa aaattaatca
     1321 aaatgcaggg caagagctgg agttatgatg agctggcctc taaaggttcg ctggccaata
     1381 ttacggccat cgcggaatcg ccgatagatc ccaatattct tttcgtcggt tcgggcgacg
     1441 gattgattca ttattccgat gatggcggta aaacgtggaa taaacccgca tccacgggag
     1501 ggttaccgga gtttgcacgc gttcatacga tcgtcgcatc gtcgcataac agacgcgtgg
     1561 cgtatgccgc ttgccacaat tttggtgacg gcgataataa accgtacttg ctcaaaacaa
     1621 gcgatggcgg gaagacgtgg acgttactca acggcaatct tcccaagaga ggttctacct
     1681 attcttttgc agaagatcac gtggatccta atttgttatt cgtgggtacc atgtttggtc
     1741 tgtatgtttc caataccgga ggtcaggact gggtagcgtt taacaatggt attcctccgg
     1801 gattgatcac cgacatgacc atccaaaaac gtgaaaacga tttggtcgtc gccacgtttg
     1861 gtcgcggtat gtatattctc gacgattata cgccgctccg tcacctgtcc aaagaaacgt
     1921 tacaaaaaga tattgccata ctgccgatca aagaggcatc catgtttatt ccttccaatc
     1981 cgtttggttt tcccggaacg ggttttcagg gtgccggttt ttttgcgaca ccgaatccgg
     2041 aaacaggcgc cgtatttacg tattacgtca aggacgatta caagtcgctg aagaaaaaac
     2101 gccgcgatac cgaaaaagaa aaacagaaaa aaggcgagga catcgaactg ccttcgtatc
     2161 aaacactgcg caatgaagcg gatgagcaag aaccgttttt tgtttttacg atcaaagatg
     2221 aattgggtca acctgtacga cgcattaaaa cttccgttca gaaaggcgtc aatcgtatcg
     2281 tgtgggattt tcgaaccaat gtatttaccc cgatttctct agcgccattt gataacagca
     2341 taccttggaa tgatccggat cggggatata tggtggttcc gggaaaatac acggtttccg
     2401 tgtcgcgcta cgaagccggt caatggacgg atctcggcgt gtcgcaatcc tttatttgta
     2461 aaccgctttt gacaccgtct ttgcctgttg cggatcgcaa taaattagac gccttcaata
     2521 aaaaagtagc cgaactgatc cgggctatca gcagtgcgga tgcctaccgt gagagtatgg
     2581 tggagaaaat accgttgctc aaacaagccg tgatcgaaac ggcaaagctt cccgaaggaa
     2641 cgtatgcagc tgtggtcgct gcagaggata aactcaaacg tattcaacgt caaatgaatg
     2701 gtgacggatt gcgtgcgctg tatgaaggtg taccgccgac atcgttgcgg gggcgcgtcg
     2761 aaatcatcac cggtaatctg tggactacca catcggatgc aacaactaca tttcagcatt
     2821 tgtatgacgt ggcggccgca caatttgacg gattacttcg tgcactccag gagactgatg
     2881 cggagatcaa acgcatagaa cagcagatgg atcgttatgg cgcgcctttt acgccgggtc
     2941 gtttaccgtc tgtaatcaaa aactaaaaaa tacaattcgt cccattaaac ggctttcggt
     3001 taaaaaatcc cggaagccgt ttttttattg tcattttttg acagaaaacc ttccgcatcc
     3061 atatttaatt tttttaaaaa aatttgcgat gaagggggtt tatgttagca cagattttat
     3121 ccgcgactac atttggtatt gatgctttta tcatcaaagt cgaagcgcat ctggagggcg
     3181 ccgtacccgg ttttattacg gtcggtttac cggataatgc cgtccgcgaa agcaaagagc
     3241 gcgttaatgc ggcaatcaaa aactccggtt ttacatttcc gatcaaacgc attacgatca
     3301 atcttgcacc ggcggatttg cgcaaagaag gttccgggtt tgatctcccg atcgccgtgg
     3361 gtattttggc cgcgcacggt cagatcgagt cccatcgtac ggaggaatac gtggtcgtcg
     3421 gtgaattgag tttggatgga agtttgcgac ccatcaacgg tgtgttgtct cttgcgcttc
     3481 gcgcccgccg cgaacatatc aaaggtatca tcgtaccggc cggtaatgcc gccgaagccg
     3541 ccatggctga aggtttagaa gttatacctg tcaacgcatt atcggatgct gttcaatttc
     3601 tcaacggaca aaaacacatc gcaccgcacc aggtagatat gtcggcactt tttgatcagg
     3661 ctccggctat gccggtggac tttcgtgatg tcaaagggca ggaacatgcc aagcgagcgc
     3721 tggagattgc ggccgccgga ggccataaca tcattatgat cggtccgccc ggaagcggta
     3781 aaacgatgtt agccaaacgt atcccgacaa tactgcctga tctgacaccg gatgaggcgc
     3841 tggaaacaac caaaattcat tctgtcgccg gttttttgcc gcagaatacg ccgctggtta
     3901 ccctgcgtcc ttttcgttca ccgcaccata cgatctccga cgcgggactg attggcgggg
     3961 gacatattcc caaaccgggt gaagtcagtc ttgcgcatca cggcgtactt tttcttgatg
     4021 aattaccgga atttaaaaaa aacgtattgg aggtcatgcg ccaacctttg gaagacgggc
     4081 atgttaccat tagtcgcgct gaaatgtcgc tgacctatcc ggctaatttt atgctcgctt
     4141 gcgcgatgaa tccttgcccg tgcggatttc acaccgatcc cggcaatcgc tgtacttgta
     4201 atccgggtat gatccagcgc tacatggcca aaatatcggg gccacttctg gatcgtattg
     4261 atattcatat tgactgtccg gcgataaaat ataaagaact ttcttccgaa ggccacggcg
     4321 aaacctcctc ggcgattcgc gaacgtgtta aaaaagcgcg gcaaattcag ttaaaacgat
     4381 ttgaatctct gcgtaaaaaa ggccagcctg tgtactgcaa tgcggatatg gagtcgcgcg
     4441 ccattcgcga attttgtacg atcgacgcac gcagtcagga attaatgaaa ctcgcgatta
     4501 ccaaactggg tttatctgca cgggcatacg atcgtatcct caaagtagcg cggacgattg
     4561 ccgattgtgc caatgctccg gccattcgcg ccgaacacgt cagcgaagca atccaatacc
     4621 gaagtttgga tcgcagttac agcggataaa tacccggctt gcctctctga ataagtttgt
     4681 gtagcataca cttccgaaca aaacgggaga ttttatgtcc gacgcatttc tcagggctca
     4741 atggaaaaac cttatcatgg ccaactatgc cgttgacccg tcgctgctcc ggccgtatgt
     4801 gccaagcggc acggaattgg attcctggaa agacacattt tatgtcagct tggtgggatt
     4861 tttatttgaa gatacacgtg tacacggtct gtcgtttcct tttcaccgga cattcgaaga
     4921 agtcaatctg agattttatg tacggcgatt tgaatccggt gtatggcgcc gcggcgttgt
     4981 ttttatcaaa gagattgttc cgcgcagtct catcacatgt atcgccaata cagtgtatca
     5041 tgagcattat gcgacgcacg cgatgcggca ccgttttacg tccgatgcgc actgtaaaac
     5101 atttgagtat agtttcaaat cggctaacgg ctggaatacg atttcagcga aaacgaatac
     5161 cgaatcaatc gcgatggctg aaggttcgga acaggagttt attgccgaac attattgggg
     5221 ttatactcgc atcaacgaaa aaaaacttgg cgcttacgaa gtgcggcatc cgcgttggga
     5281 tatatatcct gtgatggatt accatatcgc atgtgatatc ggaggtctgt atggcgaacc
     5341 ctttgccaaa acaatggccc gtaatccgga ttctgttttt gtggccaaag gatcgccgat
     5401 cgaagtaatg aaaaagaccg ttatagagca tatctaaagt gaccacgctg ttaaactttt
     5461 gtaaatacta tacgtctaat gaatgccgac aaaatcatat ttaacggcat acaagttatc
     5521 gtaattattt gtaaataatc gctttgattt tcgtggtaca aggacaaaaa aggtcgtata
     5581 atctaatagt ttataagaat tgatatttct aggatcgtaa ggaaggtgcc ttatgaaagc
     5641 ctcttttaaa aaaatactca cggttttggt tttttcgctg atttggagcg gatgttatac
     5701 ggctgtacgt caatcagggg ggtattactc cgagtttgat catgccgatg caaaacaacc
     5761 ggagataact caaattcagc catcggatga agactcatcg gatcaggatg aagaagtcac
     5821 tccatcctac gacgaagatc gtgatattta tattcaaaac tattatggcc cggaatatcc
     5881 atatccttat ggttactgga caccgggttg ggtaaattat cgtcatccgt attattacgg
     5941 cgccgggatg tattttggtc cgcgtgttcg actggcctat gtcggaacgt attggggtta
     6001 tggttattat gatccttttt atgatccgta ttattactac gacggatggt gttattatcc
     6061 gaccggttac ggccatcatg ataataatcc gatacatccg tcacaacagc gtacgtatgg
     6121 tgggcgacgc gtctatactg cctcgtcaac gcctaatctt cccggtattg tgggggtcag
     6181 tgtagcctcg tcaggcggag gcacaacgca gcgcatctat cgttccggca atcgcacctc
     6241 cggtgagtct agaattgacc aagaccgtgt gcgccgaaac taccgtaaat attacggctt
     6301 tgaaacgggt gataaggctg tcaatgcgga agctggccgt aaatcccgca atgccaatcg
     6361 tgaaacaagc acgcgtaata aagaaacacg tgaaaccaga caagtacgcg ataataattc
     6421 ccgcaataac ggaggagaac gtaacgcgcg ttccggtgaa agaaacagcg gacgctccgg
     6481 tcgtagtgaa ggtcgatccg gcgaaacccg ttcttccggg aaacgttcgg cgagaaatta
     6541 atttatgaaa tacgttggtc ttttaatcac aatccttgtg tcattgtacg gtagtgcgtg
     6601 ttataccgtt gttggtattt cccgttcgga tgatcgtatc caaaacgaag caaccgaatc
     6661 cgaacccgat tttgcggctt acgaaccgta tggtgttaca ccttcgacga tgtgggaata
     6721 ctattacgaa acaccgttcc cgtggtggta tgtgccgaat gcggatgaat acgatgcgtt
     6781 tgacgatacg acaacggcta ctgatcctac cgaaacatca tcgtattcgt catcacgcag
     6841 aaattacggg agacgtcgtg cggcgctcaa tgacgcgtcg tccgggtctt ctgtttcgac
     6901 cggttatgct tcacccgttt atagcccggg tggaacgacg ccgtcgtttg taccttcgta
     6961 ttctaccgga tcaagcgctt cctatagtag cccatccact ccggttaaaa cagactctac
     7021 agtacaatcc ggatccggta gttcggattc taattctaat tcgaccacga agactcaaga
     7081 tacgaacaga aaaagaggtt acggaaatcg cagaactgcg aaaagcggag acaagaaata
     7141 actgaggtga tgtacatgaa gtttttatcg tatgggataa gtttattttt ttattcattt
     7201 attttattgg cacaaaatga aaataatgcg gtgcggcctt ttatgaacga atttggtccc
     7261 ggtgcgcggg cattgggttt gggtggagct tattctgcaa ttgccgaaga ttataccgcc
     7321 gtatattgga atccggcagg gcttgcgcaa atacgtaaaa tggaatttta tacagcgttt
     7381 gcgcatcgaa ataatactaa tgccatcaca taccaaggta cgcgtacaga gacatccaac
     7441 ggttttacaa gcatgaacgc tttaggattt gtatttccgg ttcccacaac gcgcggaagc
     7501 cttgtgttgg ctttcggttt tcatcgcgtc aactcatttg acgattacaa tcgcgtgacc
     7561 ggttcgccga tgatcggtaa tataagtttt gatcaggatg aaaaaactac aaataccggt
     7621 agccttaacc attggagttt tgccggtgcg cttgatctga cgcgcaatgt ttcagtcggt
     7681 gcaacactca atattgttac tgggaaaaat gatctaagtg tagatttttt tcaggatgat
     7741 gagattgcca tgcttgatga cagcattgct acaaaagaag tatccttcgc tgaaaaaccg
     7801 gactattcgg ggtttaactt caaaatcgga actctatttc gccccgcaaa aaacctgcgc
     7861 gttgctctca cggtaacggc acctacaaaa atcaccgttt cagaaaatta ctccgaatcc
     7921 acgaccatca aaacggacaa caacggatat tattcggatt catggtatga gccctacaag
     7981 tatcatatta catcgccttg gaaatttgag attggcagtt catacaaata caaacttgcc
     8041 ctttttacag gctctgtcga atgggtggat tggcaacaaa cacgattcag ttctaatata
     8101 tttgatgccg atggaaaaaa tatagacgat gcgatcaaca acgcattact caatgattat
     8161 cgtacatcgg taaattatcg tatcggcaca gagatggtag tccctcagat cggttgtaaa
     8221 gttatggccg gatacgctta ccaacaatcg ccttataaaa atggccttga gatcgttaat
     8281 agcgatcacc gttatatttc tgttggtgca agttttctct tagacgaaca ggttaaaata
     8341 gacgctgctt accaatatgg atggtggaag caggcaacaa ccgataattt attggcctac
     8401 gggccttcgc cggattatta taaccggtac acctctgaaa aaataacatc taaaaaactt
     8461 ctgctcagtt taagttaccg tttttagccg atgaaaataa aaaactatcg tttgatcata
     8521 cacggcggaa ttatttccgc ctttttatta tatagcttca cgctttcagc gcaggtacgt
     8581 cctttagatc aggcgatatt gcggcccgga tcaggcgttt attccgatct ggcgcacatc
     8641 gagggagaaa caatcaaaga tatggtggcc gccttgtctg cggattctat ggaaggacgc
     8701 gcgacgggaa cacgtggatt tgataaagcc gcacgatggg tgaaagaatt ttttgttcaa
     8761 cacggcattc aacctttacc cggaggatat tttcaagatt ttacggtatc gacttctgta
     8821 ttaaaagaac ggctgcattc aaacgaatgg tcaagcgaaa cggcttcgac aaaaaacatt
     8881 atcgggtata tagcgggaaa tgattctctc cggcgcaatg agtttattgt tttaacggca
     8941 cacctggatc atctgggaaa agaaggtgat tcgatctatg ccggtgctaa cgataatgcg
     9001 agcggggttg catccgtgtt gcagattgcg cgatggatca aagaatccaa aaccgatttg
     9061 gggcgttctg tggtttttat tttgtttagc ggcgaagaag ccggtttact agggtcttat
     9121 cattttatag aaaacccatg gattgatctt aacaaaatcc gcctgttgat taatctcgat
     9181 ctggttggtt cgggtcgtga cggtatcatg ttacaaggat cggaaaacta tcccgattat
     9241 gcgacgcgta taaaaaaaat aaatgatgag cggtttggct ttgaaatgag tacgcgacct
     9301 aattcgccga actcagacca ctattatttc aacaaaatag gattaccggc tttttttgtg
     9361 tatgcctatc gtggcacgat gccctatcat gcacccggcg atacctcaga aaagattgat
     9421 gaaaaggttt tagagaatgt tacccggtgg gttgcggcga tggttgtagc atttggaaac
     9481 gaataacccc gaaatatttt tacgtcgttc tttacgatcc gaaaaaaata cttcggggtt
     9541 ttttagctat acagtttact ttctcggaac tggtaccggt acgggttcag gttgctttcc
     9601 aaaaagtcgt tttaagattt ctatcagttg ttccataaac ctttgatttt agtttcgggt
     9661 aagaatgcgt atgtcgccac cggaagtgtg tgcgcgtatc aattcgccgc caccgttgat
     9721 tgtgccacgt actttgtttt cgcgcatttt cccgttgacc atgatcggta aatcaaactc
     9781 aacctcgcca ccgctcgtgg aggcgtctat cgttgcggca atgtttttgg ggagatacag
     9841 cgcgatgtga ccgcctgatg tttccacatt aacgccttta ttattaccgc tcacatacac
     9901 ctcaatatgc ccgccggatg tttcagcatt ggtgcgaccg ttgatgtctt cgatacggat
     9961 actaccgccg gatgtggaaa catccaaatc gccgttaata ttactcatgc tcacatttcc
    10021 accggaggtt ctcatgttga gattgcctgt cgttgatttg gcacgaatat taccaccgga
    10081 agttttgagt tctgcattac cattgatatt cgtcaaatca agattaccac cggatgtccc
    10141 accttggatt aaccctttta cgtcctgaat gacgatattt ccgccggccg tgcctaaaga
    10201 aacattatat tgcgaaggaa cattgacgat ataatcaaca tccattgagt tactattatg
    10261 gccaaagaaa ttgttttttc tcatccagtt gccgcgaata gtcacatcgt tacccgtttt
    10321 ttccgccgta atttcaaaat ctttaagatc gtcatccgaa ccgcgcatac gcgccatgat
    10381 agaaaccttg tcatcggaag agcccacgat cttgatcgaa ccaaagtctg tagtcaaggt
    10441 aagttgacca cccggtgata cgggaaatga acgtgtaaat tctttaaccg caacgaccga
    10501 acccgatgcc gctgtgttat cggtcgccga aacagtaaag gctgccaaca gtgtcagcgt
    10561 gagaaataat tttttcatat acatcccttc tcgttttttt ggacttttta taaaacgaga
    10621 ttcggcggaa aaagtttcgg aaataaattt gtatttcgaa ccaagcccaa aggccaaaaa
    10681 aaagaggcta tcttaagaca gcctcttttt atatcgtaat tttgatttta aattacttaa
    10741 cgaaattcat cacgtcttta ttgacgacgg gtgttcctga tgaataatcg taaaagcctt
    10801 tgccgttctt acggccataa aagcccgcta aaaacatttt gcgaagcagc ggggggcaag
    10861 aatacatcgg ttcgccgtat tctttgaaca tgatttcagc gatacggtag gtcgtgtcaa
    10921 taccgacaaa atctaaaagc gtcagcgggc ccatgggata accgcaaccg agcatcatac
    10981 ctttgtcgat atcttcgatg gaagcgatgt tgttttgtac ggcacggatc gcaccgagga
    11041 gataaggcac taaaaggaaa ttgaccacga atccggaatt gtcttttgtt gcgatcggtg
    11101 ttttgccaaa cgctttgccc aattcgaaag ccctattaaa tgtttcttcc gacgtacgta
    11161 cggcttttac gatttcaacc agtttcatga ccggtaccgg attaaaaaag tgcataccgc
    11221 agaaacggtc gggacgattg gttgcggcgg ccatttccgt cacggtaagg ctggatgtat
    11281 tggaagcaaa aatcgtatgc ggaggacaga tcttatcaag ataagcatat gtttcgtttt
    11341 taatttttac gtcttcgata atggcttcga tgataaagtc acagtttttc agatcatcta
    11401 aactggtcgt tccggtgaga ttagcgatgg ttttatcttt ttcttcggcc gtgattttac
    11461 ctttggcggc attacgttcc aattgttttt tgatatttcc gacgccttta tcaatcacgt
    11521 cctggtttac ttcgcgaacg atagttttaa atcccgatgc agcggcgacc tgagcaatac
    11581 cggcgcccat aaggccgcaa cccaacacgc cgacggtttt gatgtctttg acttccatgc
    11641 gaatactcct cttggatggt tatgtttatt ttttttgtat gatctttgta cggacgatcc
    11701 acgcgtcggg ataaccggct tgtttggctt cggctttctt catttcggca tcgtcgcgtt
    11761 gtataaaatt tcccgcgcga actttataat atggcggttc ataatcaaga taaatttcgc
    11821 cgctcaaacg aacgcttacg gagtctctta tttccaatgc ttcataataa tccgttgtgg
    11881 aaataagttg cacacggtat ccgataacag aggtttcttt gccggaagat gtaactacaa
    11941 cttcggccgg ttcggtataa ggttgaatgg ttacttcgta tgttcgcggt tcgatgatcc
    12001 acggatcatt gagcattgta aaatcaaacg ctttttccga atggtcttgc tgagaaatgg
    12061 gctgtatgat cggggacggc tctaccgatg cggtttgttt aacattgtgc gcaccgcacg
    12121 acaaaataaa attcatacca aaaaatatgt atccgatttt cttcatgccg ccaaatgcgc
    12181 tttgagatca gagagacgtg ggatgatcaa atccggattc aaggttgcta attcatcggt
    12241 cgaatgcatt ccggatgtta cggcgacaac aaaaacattc gcagattttg ccgctttgat
    12301 atccaaaggg ctgtcgccga tgtaaatcgt tgtttcgggt gtgccggcta attcctcaat
    12361 ggctttgatc aatccttccg gatgaggctt gagatgcgtg acgtcttcgt atccgatgac
    12421 aatatcgaaa aaatcatcca tttctttttc gatcagtgtt tcccatatcg tggagcgtct
    12481 tttagtagaa acaatgccca atgtataaag ttcgttatat aagaactcca atgtttcacg
    12541 tacgccgtcc agcatgaccg tattgcgaac catagcaccg gtatcggcat gctccataaa
    12601 aagagattta caggtttgta ttttttccgt atccgacgga tcaataaaac gagcaaatgt
    12661 attttcgagc gaatggccga tcatacggcg aatggcgtcc ggctgtccgg ggggggcgcc
    12721 cactttttcc agagcaaaat taacacaggt gatgatacct tcggaagaat cagcgagcgt
    12781 atagtcaaaa tcaaaaagaa tttgatgaat atttttaatc accgatttta aaacgcttaa
    12841 cagttttttg aagtgtcgtg gtaaggttat taagatattc ggcggacttg gctatttcgt
    12901 tgataccgcc ggcgatttgc tgtgttgccg tattgatcac tttgacatta tccgtgatct
    12961 gatcgcccgt aacggattgt tcctggcttg agacggctac ctgactgacc atcatggtga
    13021 catttttgaa tacgtctgaa atttccttca agctagactt ggcttcttgt gaaagacgta
    13081 tgccattggc tgcttcttca ttgctacgtc ccatcagatt gacggcttcg tttacgttga
    13141 cctgtatctt ggatatgatg tcattgatct cagtggtaga atgagccgta cgctcggcca
    13201 gttttttgat ttcatcggcc acaacggcaa aacctttacc tttttcaccg gcgcgggtcg
    13261 cttcaatcga cgcgttgagt gcgatcaggt tcacttgttt agtaatgtcg tcaatgatgc
    13321 ttacgacctt agtaattttt cgactggatt cggataaaga attgacaacg ttggaagact
    13381 gctgaaccat ggccagcatt ttttgcatac cgtccaccgt ttcatcaatc acttgcgaac
    13441 ctttatcggc gctactttgt gcgtggatag ccatttcttt ggtattgcca atattgtgag
    13501 cgatctcata actggttttg ctcatttctt cgatagcggc gg
//
LOCUS       DAOQNQ010000058        13427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1632_length_13427_cov_6.850882, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000058 DAOQNQ010000000
VERSION     DAOQNQ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13427
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1632_length_13427_cov_6.850882"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="PK754_05610"
     CDS             complement(<1..148)
                     /locus_tag="PK754_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004727351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HNB09012.1"
                     /translation="MKHNVIIEPFRIKMVEPIYMTSEEERNQLLHQAHYNPFLLHSDK
                     VMIDF"
     gene            complement(231..689)
                     /locus_tag="PK754_05615"
     CDS             complement(231..689)
                     /locus_tag="PK754_05615"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HNB09013.1"
                     /translation="MLRSNRLPALLSLLSVLIGSCTSGPEPIAYGQDECTHCRMGITD
                     RRFGSEWVTEKGKIFKFDSIECLVKYYYQQKSANDQGTVWVTAFDQPEAFIPAKDAHY
                     LRSEKRPSPMGMFLSAYNGNAAAENAQKENGGDIMSWEQLKVYIEKENRF"
     gene            complement(655..1281)
                     /locus_tag="PK754_05620"
     CDS             complement(655..1281)
                     /locus_tag="PK754_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09014.1"
                     /translation="MNKRQKILIVVASLMLTGIYFTPLWKITLDAPQYPEGLGLYIWV
                     NKISGENQHDLRTINGLNHYIGMKEITPESIPELKIMPYFVGFLIITGFIVAFVGNRK
                     VLGVWAFIFFAVLIAGLIDFYLWEYDYGHNLNPHAAIKIPGMSYQPPLIGSKQLLNMH
                     TTSLPSIGGILAGLSFLITLYVLFVSRKNTSITEEKNHVAVKPITGAA"
     gene            complement(1342..3306)
                     /gene="nosZ"
                     /locus_tag="PK754_05625"
     CDS             complement(1342..3306)
                     /gene="nosZ"
                     /locus_tag="PK754_05625"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HNB09015.1"
                     /translation="MFRKPSARIVYASLVVLALAAVWIGCPAKKEATIGSGDAASKVF
                     VPPGSYDEFYAFLSGGFSGQVSVYGLPSGRLFKTIPVFAVNPENGWGFNEETKGMLQT
                     TDGFIPWDDSHHPELSMTDGTPDGRWLFINANNSPRIARIDLGTFRTEEIIEIPNSGG
                     SHASPFTTINTEYVVSATRFSVPTPQADVAINSYKQNFKGAISFVKVDPKEGHMDLAF
                     QILVPGFDYDLSHAGKGPSHGWFFFSTYNTEQAHTLLEVNASQNDKDFIMAVNWKKAE
                     EYIKQGKAKSWTSEYYINRYDDNKHTTISEKKKQVMMLDPKDCPDMLYLMPCPKSPHG
                     ADVDPSGEYIVGGGKLATVIPVFSFSKMIKAIEGKAFDGDRDGIPVLKYDAVLAGEVQ
                     EPGLGPLHTEFDGKGYAYTSHFVSSEVVKWKLGTWEVVDRMPTYYSIGHLMIPGGDSK
                     QPWGKYVLAMNKITKDRYLPTGPELTQSAQLIDISGDKMRMLSDFPTIGEPHYAQGIA
                     ADLVRKHEVKIFDINKNEHKYATKSEKDAKVVREGKEVHVYMTASRSHFVPDNIEGVK
                     VGDVVYFHMTNLEQDWDVPHGFAIKGNNNAELLIMPGETATVRWEPKTPGVYPMYCTD
                     FCSALHQEMQGYVRVSAAGSNTALTFSTGK"
     gene            complement(3376..3879)
                     /locus_tag="PK754_05630"
     CDS             complement(3376..3879)
                     /locus_tag="PK754_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB09016.1"
                     /translation="MKSYVLALAIFAATISMTACGSSNEEAKASWGEAANKPAKPDPY
                     AGLTAFEKEHGIGPVKEELKLEAINTTLVKKGEKAFETKCYACHRLDTKLVGPPLQEV
                     LKTRKPEYVMNMMLNPEGMIKNHPDAKKLFAQFLIPMANQNLTMDECRAILEYLRHAN
                     ENAKKPS"
     gene            complement(3905..4651)
                     /gene="ric"
                     /locus_tag="PK754_05635"
     CDS             complement(3905..4651)
                     /gene="ric"
                     /locus_tag="PK754_05635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HNB09017.1"
                     /translation="MKSFEDMPLGQIVLEYPRSAEILEHYRLDFCCGGKKILSDACRD
                     KDIALADVCRDIEKAIQSPSQGQQPKPIHEQPAGFIVEYIQSNHHTYMRHAIPLIERH
                     LNKVAQVHGDHHPEMVTVREAFEALSSELKLHLQKEEVILFPYVCELEDHITHHAPIR
                     PNGFGTVLNPIHVMENEHAFAGQQMEKIRTLTADYHVPVDACTTYRLLLHELQEFEQD
                     LHKHVHLENNVLFPKAVMMEKQIFNKTSHN"
     gene            complement(4715..5179)
                     /locus_tag="PK754_05640"
     CDS             complement(4715..5179)
                     /locus_tag="PK754_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNB09018.1"
                     /translation="MLLTKSCLYGIRAAMYVASLGHKEYVPIQQISENLNISFHFLTK
                     ILQQLTENNIMISYRGPKGGVALARDPKDISLTDIVEAIDGPNIFDQCIFGLPGCGNL
                     APCPLHSEWAKHREKINAMFENATLNTLADKINTFNFRIADEGYEEEKKQPK"
     gene            5528..>13427
                     /locus_tag="PK754_05645"
     CDS             5528..>13427
                     /locus_tag="PK754_05645"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HNB09019.1"
                     /translation="MKTLLTMIALIFSASDMYSQTALTGGITQSGNFTAAGQTATYSY
                     NQSVSYWSVVGIRPPSTSDFDIELSANTNFSPTLASSTLGTGKVDFIVADYNHSPTGA
                     QYVRLKDYSGAGTYYVEYESGAHTLGVPSNSGTLSWTSNEIVKVWDVYLQAGVTYTFK
                     LRTLSGLQDYGISLYKSNTAAYYAGRSSAAATSDITASGTDEYFSYTPSVTDYYGFVV
                     WSNDNYGTSGTYDITVSTLATNNPLSSAASPGQFSYYQTNTNWSVVALRSGSGANDNL
                     SLYTNSSYTTLATQSALASGIEIIATENDTITQGYFRYPKVDRTGGTSQYTLEWDESS
                     GYIGLGNYTTTAWNANTVAKVYNLSMSQGAKYRIKLHTVSGSVDAGLLLFKPNGSLSS
                     QARSAALQSADANGAGLNEEIILTAPVSGSYALVVYSNTATAGSFDLSFNQEILAASN
                     VYGQGVKHYAFDQQNAAWSVVGVRPPSNGNADIVLYNNHTYTTPLATSNTAGTGQVDF
                     VVGDQHHTAKGIYYVSTDDPVNPSRVNYTTQWEGGNESLTAPGTNSGYAWAKTDVVKI
                     WDVQLVSGINYTFNLSVSGGTPNLGMAIFRSSSTTESFYFGRSGAAAIADVNGNGGGE
                     SFSYTPSGALGTLDHYGIVVWSNDTTSAGSVRIDIAQPLMTSATPVSASSSPGLYAFN
                     QSVSGWGVIALRPQSFEDFDLELHGASDYNNLLAQSTLGTGAVEFIAGDFRAGHAPLK
                     TYFTKAVQNSGKGPYAIEWEGTNETLPVEGNSGTISWGTTAIAKVYNVPLVGGRTYKI
                     KLDFLTNAFDAGMALFQPSTGNNFFPRGSQAMLADVAGAGNESMTFTAPMTDTYGLVV
                     WGNTQPASAATFAIDINQPPVVSVPIPDVNFSEDQGSGLVANSNLNGNFSDIQPLSYS
                     ASVLKGGGITASVAGSTLKVSTVSDSVGQYPVVVTANDGTFFTSDTLIVTLAPVNDLP
                     YRSALIPDPKIAEDEQSVFASKLSGYFTDIDNPVLSFGVTALNSGVSAYVSNDSLYLS
                     GQQDFNGNANLRISATDGSFSVYDTINVTVTPVNDVVVLSQTIADTTLPEDFSGLVFI
                     RKLSNIFQDIDNDSIAFRATVTGGAIGAVIQHDSLYVTSSPDFYGQSTISILASDGSS
                     RDGLGKLSDYFSESANTVETSFRVTQSPVNDPPMRSNPIVDKTYAENFNKVLVQKLSL
                     VFTDIDNSSLTYGVSTISKDQDVFGFISNDSLYVLSSKDSNGVDSIRVTASDGMFTAA
                     DTFRVNVTNVNTAPTVTSAINNVTVSEDFGKNFVRLLTTVFSDIDGNALSYSVSVLPG
                     DNDVTAMISNDSLYILSSVDSSGGDTLRVTASDGQYSVQTQFTVTVNPVNDGPKIISA
                     LRDTTYNEDFPKTLVRRLSSVFLDADHPSLTYAGSVLSGGASVVVSNDSLYATGTLNA
                     NGTAAIRVSATDGTLSVADTFQINIKPVNDAPVFSKTVADTSLLEDFGKVFYRKLSTH
                     VSDVDNASLTYNAYVLSGSIQVQISTDSISLISQADYYGAATIRWTASDGSYTLADTF
                     TVNIANVNDGPVRVTALRDTVFSEDFGHVFVRQLGTVFSDVDNAALIYTATVLSGNAT
                     TAIPSGKDSLYIQSSTDFNGDLIIRTTASDGALSVSDTFTVTVSPLNDGPYRTVNISD
                     ISVQEEFSKTYVAKLSQHFSDNDDASLSYAVINNPSSFDAMIGGDTLYITSRPDSNGV
                     YSLVVRTKDASLSAYDTLTVTINNVNDAPFVATPLPDTDVDEDFEKYFVADLQQIFSD
                     IDNSTPAYSVTSLTPSVVQAQVSGDSLYLISVPAVYGSGQIRVTADDGQASAADTITV
                     TVNNVNNSPYVANVLRDTVLQEDFGKVFLVTLSSHFSDIDNVSLQYSARALQNGLKAQ
                     IHQDSLYVLSDADYYGIVEVEITAADGYLASLDTMQIVLTNTNDAPYMAASAPDIVLN
                     EDFGTWTYGNAGTYFADIDNALTYGVEVLSAGVNAAVFGDTIKLYSTARFYGTSQIRL
                     FATDGQFTISDTMAVQINNVNNTPYVAASVPDTTILEDFGYTWIRTLSPYFQDYDNAT
                     LTFAATALTPGVSVETGHDTLYIQSQSDFFGSVAIKLSASDGEYTTTDTMAITIQNVN
                     DSPALSHPMRDTSLAEDFGKTALYQLTDIFSDIDNGSLSYGSEALAAGVTGSISGDTL
                     YVHSTPYFNGDADVRIFATDGEYQVSDTIRISVTGVNNAPYRLAALPDTSLPEDFGKM
                     PYRVVSQFFSDVDNMTLTYSAQVLQGQIGVQTVNDSLYFISVQDFNGAASVKVTASDG
                     FFTVHDTLVLTVTPMNDAPASALPLQPGPSVLTNNLRPTFNWHASKDIDGDAITYTLT
                     VARDNAFSDVILTQQSTDTVATASADMDTTAVLFWRITANDNHGGVVIGPATSFSTDA
                     RKAFFQIGVLANTVSPLHVGLYVRSTKQLNGNPAGQFELSENGNVISAHNDTFEKLTA
                     HTEPTYYTNYVIRQSGTLKISVTGTDMVGNVTVAQRNYAVDVLARNKPFATASDGIVW
                     SGTAMSEGMTAIAREDADAGPMNKNTSASLYTDEAISPVYEMIVTADVKGKLTLTYSD
                     DVISNLHRIISGEVDERLIGIYAEENGQWVYIGGQRE"
BASE COUNT         3496 a         3245 c         3031 g         3655 t
ORIGIN      
        1 aaaaatcaat catcacttta tcagagtgaa gtaaaaaagg attgtaatgc gcttgatgta
       61 aaagttgatt gcgttcctct tcgctcgtca tataaatggg ttcaaccatt ttgatacgaa
      121 aaggctcaat aataacattg tgcttcattg tctttttcct gatagttttt cggttacaat
      181 ggtcacaata tagattttaa tgcttctttt tttctgacaa aagtcatatt ttaaaaacga
      241 ttctcttttt ctatgtaaac tttgagttgt tcccaactca taatatcacc accgttttct
      301 ttctgcgcat tctcggcggc tgcatttccg ttatacgcgg agagaaacat acccatcggg
      361 ctgggtcttt tttcgctgcg caggtagtgc gcatctttag ccggaataaa tgcttcgggt
      421 tgatcaaatg ccgtcaccca aaccgtacct tgatcattgg ctgatttttg ttggtaataa
      481 tacttcacca aacattcgat ggaatcaaat ttgaaaattt ttcctttttc agtaacccat
      541 tcgctgccaa aacggcgatc cgtgataccc atacgacaat gggtgcactc gtcctgtcca
      601 taggcgatag gttcgggccc ggaagtacag gaccctatca gtaccgataa aagactaagc
      661 agcgccggta atcggtttga ccgcaacatg gtttttttcc tccgtgatgg acgtgttttt
      721 acgtgatacg aaaagaacat acaacgtgat caaaaatgat aatcctgcca gaatgcctcc
      781 gatactcggt agcgatgtcg tatgcatatt cagaagctgt ttgcttccga taagcggcgg
      841 ttgatagctc ataccgggaa ttttgatcgc cgcatgcggg ttgagattat gaccgtagtc
      901 gtattcccac aaataaaaat caatcaaacc tgcaatgagc acggcgaaga atataaatgc
      961 ccacacacct aaaactttcc gattaccgac aaaggcgaca atgaatccgg taataataag
     1021 aaaacctaca aaataaggca tgattttaag ttccggtatg gattcgggcg taatttcttt
     1081 catgccgata tagtgattga gaccattgat agttctgagg tcgtgctgat tttcgccgct
     1141 gattttattg acccaaatat agagaccgag tccttcggga tattgcggcg catccagcgt
     1201 gattttccaa agcggcgtaa aataaatacc ggtcagcata agcgaagcca ccacaattaa
     1261 tattttttgt cgtttattca tgacacacct tacaaagaaa ataaagggga ggttttttga
     1321 agcctccccc aaaatccaaa cttatttacc tgtgctaaat gtcagcgcgg tattggaacc
     1381 ggcagccgaa acgcgaacgt aaccttgcat ttcctgatgc aatgcagagc agaaatccgt
     1441 gcaatacatc ggataaacgc cgggtgtctt cggttcccaa cgtacggtag cggtttcacc
     1501 gggcatgatc agaagttccg cattgttatt gcctttgatg gcaaatccgt gcggcacatc
     1561 ccaatcttgt tcgagattgg tcatatggaa ataaaccaca tcaccgacct tgacgccttc
     1621 gatattgtcc ggcacaaagt gagagcgcga agcggtcata tacacatgca cttctttgcc
     1681 ttcacgaacg actttggcat ctttttcgct cttggtcgca tacttatgtt cgtttttatt
     1741 gatgtcaaaa attttgactt catgtttgcg aacgagatcc gccgcaatgc cttgagcata
     1801 gtgcggttcg cctatcgtcg ggaaatccga caacatgcgc attttgtcgc cgctgatatc
     1861 gatcaactga gcggattgcg tgagctccgg acccgtcgga agataacggt ctttggtgat
     1921 tttattcatc gctaatacat atttgcccca cggttgttta ctgtcgccgc cggggatcat
     1981 gaggtggccg atcgaatagt aggtcggcat acgatcaacg acttcccacg taccgagttt
     2041 ccatttgaca acttccgatg atacgaagtg ggatgtatac gcataacctt taccgtcaaa
     2101 ttcggtatgg agcggtccga gacccggttc ttgtacttca ccggccaata cggcatcgta
     2161 tttgagaaca ggaataccat cgcgatcacc gtcaaacgct ttgccttcaa tggctttgat
     2221 catcttgctg aaagaaaata cgggaataac tgtagccagc tttccgccac cgacgatgta
     2281 ttcgccggac ggatccacat ccgcgccatg cggagatttc gggcaaggca tcagatacaa
     2341 catatcggga caatctttgg gatccagcat catcacttgt tttttctttt cggaaatcgt
     2401 ggtgtgctta ttgtcatcat aacggttgat ataatattcc gaagtccagg atttcgcttt
     2461 gccttgtttg atatattctt cggctttctt ccagtttacc gccatgataa aatctttatc
     2521 attctggctg gcattgactt caagcaacgt atgcgcttgt tccgtattgt acgtggagaa
     2581 gaagaaccat ccgtgggacg gacctttacc ggcatggctc agatcatagt cgaaacccgg
     2641 caccagtatc tggaacgcga gatccatgtg gccttcttta ggatcaactt tgacaaatga
     2701 aatcgcgcct ttgaaatttt gtttgtacga attgatcgct acgtcggctt gcggtgtcgg
     2761 cacgctaaaa cgtgtcgccg atacaacgta ctccgtattg atcgtcgtga acggcgaagc
     2821 gtggctgccg ccgctattgg gaatttcgat gatttcttcg gtacggaatg tacccaaatc
     2881 aatacgcgcg atacgcggcg aattattggc attgataaaa agccaacgtc cgtccggagt
     2941 accgtcggtc atggataatt caggatggtg gctgtcgtcc cagggaatga agccgtccgt
     3001 cgtctgaagc atacctttgg tttcttcatt gaagccccag ccgttttcag ggtttacggc
     3061 aaaaacaggg atcgttttga aaaggcgacc gcttggcaga ccatagacag acacctgacc
     3121 actaaagccg ccggagagaa atgcatagaa ctcatcataa cttccgggtg gaacaaatac
     3181 tttggatgcg gcatcaccgc taccgatcgt tgcctctttt tttgcagggc acccgatcca
     3241 gaccgcggct aatgcgagca caaccaacga cgcataaact atgcgtgcag atggttttcg
     3301 gaacataaga gcctcctcga tcactcagat cgtttttttg ttttatgtaa acgatggtat
     3361 aagccatcga aatcatcacg aaggtttttt agcgttttca ttagcatggc gcagatactc
     3421 taagatggca cggcattcgt ccatcgtgag attctgattg gccatcggaa tcaaaaattg
     3481 cgcaaacaat tttttggcat ccggatgatt tttgatcatg ccttccggat tgagcatcat
     3541 attcatcacg tattccggct tacgggtttt gagtacttcc tggagcggcg ggccgaccag
     3601 tttggtatcc aaacgatggc aggcgtagca cttggtttca aaggcttttt cgcccttttt
     3661 gaccagtgtc gtattgatgg cttccaattt cagttcctct ttgaccgggc cgataccgtg
     3721 ttctttttca aatgccgtca atccggcata cgggtccggt ttggcaggtt tattggcggc
     3781 ttctccccaa gaagctttgg cttcttcatt ggatgaaccg caagctgtca tagatatggt
     3841 ggccgcgaaa atggcgagcg ctaacacata ggatttcatg atattttcct ttgaggttta
     3901 tggattaatt atgtgaggtt ttgttgaaaa tttgtttttc catcatgacg gccttgggaa
     3961 acaagacatt gttctcgagg tgcacatgtt tatgaaggtc ttgttcaaac tcttgcaact
     4021 catgcagaag cagacggtag gtcgtacaag catccacagg aacatggtaa tcggccgtca
     4081 acgtgcgaat tttttccatc tgctgtcccg caaacgcatg ttcgttttcc atgacatgga
     4141 tcggattgag cacggtgcca aacccattgg gacggatcgg cgcgtgatgc gtgatgtgat
     4201 cttccaattc gcagacgtag ggaaaaagaa tcacttcttc tttttgcaaa tgcaatttaa
     4261 gttcggatga tagcgcttca aacgcttcac gaacggttac catttccgga tggtgatcgc
     4321 catgaacctg tgccacttta ttaaggtgac gttcgatcag tggaatcgcg tggcgcatgt
     4381 aggtatgatg attgctttga atatattcga ctataaatcc cgcgggttgt tcatggatcg
     4441 gcttaggttg ttgaccttga gagggtgatt gaatagcttt ttcgatatca cggcatacat
     4501 ccgccagcgc gatatcttta tcacggcaag cgtcggataa aatttttttc ccgccacaac
     4561 aaaaatccaa acgatagtgt tcgagaattt cggctgaacg agggtattcc aatacgattt
     4621 gaccaagagg catgtcttca aatgatttca tttttaatcc tttagaagat ttaagagtat
     4681 tttatcttta atatgaataa aaaaattccc cgcttcactt cggttgtttt ttttcttcct
     4741 catatccttc gtcggcgatt cggaaattga acgtattgat tttatccgcc agtgtattga
     4801 gtgtagcatt ttcaaacatc gcatttatct tctcacggtg tttggcccat tcactatgca
     4861 aggggcaagg cgcaagattg ccgcagccgg gcaatccaaa aatacattga tcaaaaatat
     4921 taggaccgtc tatggcttcg acaatatccg taagtgaaat atctttggga tcgcgcgcca
     4981 aggccactcc gcctttgggt ccacggtagg atatcatgat attattttcc gttagctgct
     5041 gcaaaatctt ggtcaagaag tgaaatgata tattgaggtt ttcagaaatc tgctgaatcg
     5101 gcacatattc tttatgacca agagacgcca catacatcgc ggcacgaatt ccgtataaac
     5161 aacttttagt tagtagcatg gtaatagttt cttttccgga taaatctttg ctctattcct
     5221 gtatttcaga gtcttttgtc ttaaaataag atattcaacc tcatttgtca agtattttgg
     5281 tctggttttt caattttttt ttgaaaatac aaaaaatcgg gggtggcgtc attttattga
     5341 cgaattattt aaaatttttt acaaaaccta cacgcttatg tagcctcatt tgtgagctaa
     5401 atcacacgat attgtgccga gtagacttac cctaaatttg attaaaaata aacaattata
     5461 cctctgtttg agggaacctt tcaccgttca cataacgtca tgtttttagc atgcttttga
     5521 ggtaattatg aaaaccttgc tcacgatgat cgctttgatt ttttcagcaa gcgatatgta
     5581 ttcacaaact gcgcttaccg gcggtattac gcagtccggt aattttacgg ccgccggcca
     5641 aacggccacc tactcctaca accaatcggt tagttattgg agtgtggtgg gtattcggcc
     5701 gccatcaaca tccgactttg acatcgaact tagcgccaat accaatttca gccctacact
     5761 cgccagtagt acattgggta ccggcaaagt agatttcatc gtagccgatt ataatcattc
     5821 gcctacaggc gcacaatacg ttcgtctcaa agactatagt ggtgcaggta catactacgt
     5881 cgaatatgaa agcggcgcac atacattagg tgttccctcc aatagcggta cactatcctg
     5941 gacatccaac gaaatcgtca aagtttggga cgtgtatctg caggccggtg tgacttatac
     6001 atttaagctc cgcacacttt ccggccttca ggattatggc atatcgcttt acaaatccaa
     6061 taccgccgcc tattatgcag gccgcagttc ggctgccgcc acatccgata ttaccgccag
     6121 cggtacggac gaatactttt cctatacacc gagcgttacg gattattacg gctttgtcgt
     6181 atggtccaac gataattacg gcacaagcgg aacctacgat attacggttt cgacattggc
     6241 caccaataac ccgctatcca gcgcggcttc gccgggtcaa ttttcatatt atcaaacaaa
     6301 taccaattgg tccgttgtcg ccttacgctc aggtagcggc gccaatgaca atctctcgct
     6361 ttataccaat tcatcctata cgactttagc tacgcaaagc gcattagcct cgggtatcga
     6421 aattattgcc actgaaaatg ataccatcac ccaaggttac ttccgttatc ctaaggtgga
     6481 tcgcaccgga ggcacttcac aatatacttt agagtgggac gaatccagcg gatatatcgg
     6541 tttgggaaat tatacgacga cggcatggaa cgctaacacg gtcgctaaag tgtataacct
     6601 gtccatgtcc caaggcgcta aataccgaat taaattgcat accgtttcag gtagtgtgga
     6661 cgcaggcctt cttcttttca aacccaatgg ttcactgagt tcccaagcgc gatcggccgc
     6721 actgcaatcg gccgatgcca atggcgccgg actgaatgaa gagatcatac tcaccgcgcc
     6781 ggtttccgga tcgtacgcat tggtcgttta cagcaatacg gctactgccg gttcatttga
     6841 tctttctttt aatcaggaaa tactcgccgc ttccaacgtg tacgggcaag gtgtcaaaca
     6901 ttatgccttc gatcaacaaa atgccgcatg gtccgtagtc ggcgtgcgcc cgccatccaa
     6961 cggcaatgcc gatatcgttt tgtataataa tcatacctac accacgcctt tagcgaccag
     7021 caatacagcc ggcacaggcc aggtggattt tgtcgtcggc gatcagcatc acacagccaa
     7081 aggtatttac tacgtaagca cggacgatcc cgtgaatccg agccgcgtaa actataccac
     7141 gcagtgggaa ggcggcaatg aatcgttaac ggcgcccgga acaaatagcg gttacgcctg
     7201 ggccaaaaca gatgtcgtaa aaatatggga tgtacaattg gtctccggaa tcaattatac
     7261 atttaatctg agcgtaagcg gcggcacacc caatcttggc atggcgatat tccgctcttc
     7321 ttctactacg gaatcatttt attttggccg ctccggagcg gccgcgatcg ccgatgtgaa
     7381 cgggaacggg ggaggcgaat cattttccta taccccgagc ggcgcattag gcacactcga
     7441 tcattacggt attgtcgttt ggagcaatga taccacatcc gccggaagcg tccgcattga
     7501 catcgcccaa ccgcttatga ccagtgcgac acccgtttcc gccagcagtt ctcccgggct
     7561 ttacgcgttc aatcaatccg tctccggatg gggcgtcatc gcattacgac cgcagtcttt
     7621 cgaagatttt gatttagaat tgcacggcgc ttccgattac aacaatcttt tggctcaaag
     7681 caccttggga acaggtgctg tagagtttat cgcgggcgat tttcgtgccg gacacgcccc
     7741 gcttaaaacc tatttcacaa aagccgtaca aaacagcggc aaaggccctt atgcgatcga
     7801 atgggaaggc accaacgaaa ccctacctgt cgaaggtaat tccggtacga tcagctgggg
     7861 taccacggct atcgctaaag tatacaatgt gcccctggtc ggcggacgca cttacaaaat
     7921 caaattggat tttctgacca atgccttcga tgccggaatg gcgttattcc aaccgtcaac
     7981 aggtaataat ttttttccac gcggttcgca ggccatgctc gccgatgtag ccggcgcagg
     8041 caatgaatct atgacattta cggcgcccat gacggatacc tacggccttg tggtttgggg
     8101 caacacacaa ccggcttcgg ccgccacgtt tgccatagac atcaatcagc cgcccgtagt
     8161 gagcgtaccg attcccgacg taaatttttc agaagatcaa ggcagcggac ttgtggccaa
     8221 ttctaacctc aacggaaatt tttccgatat acaacctttg agctattcgg catccgtact
     8281 caaaggcggc ggtatcacgg ccagcgtggc gggcagtaca ctcaaagtca gcacggtttc
     8341 tgattccgtc ggccaatatc ccgtcgtcgt gacggccaat gacggaactt tttttaccag
     8401 cgatacactc attgtcactt tggcgccggt gaatgatctt ccataccgtt ccgcgctgat
     8461 accagatcct aaaatcgccg aagatgaaca gtctgttttt gcatcaaaac tcagcggtta
     8521 ttttacagat attgataatc ccgttctttc ctttggcgtt accgcactaa acagcggggt
     8581 ctcagcttat gtgtccaacg attcactgta cctgagcggc cagcaagatt ttaacggcaa
     8641 tgctaattta cgaatttcag cgacagacgg atcttttagc gtctatgata cgatcaatgt
     8701 gactgtaaca cccgtcaacg acgtagtggt attatcgcaa acgattgccg acaccacgtt
     8761 gcccgaagat tttagcggat tggtttttat ccgtaaatta tcgaatattt ttcaggatat
     8821 cgataatgat tcgatcgcct tccgcgcaac ggttaccggc ggtgcgatcg gcgcggtgat
     8881 acagcacgac agtttgtatg tcacaagcag tcctgatttt tacggtcaat ctaccatctc
     8941 tatcctggca agcgacggtt cctcacgtga cggattggga aaactcagtg attatttcag
     9001 cgagagtgcc aatacggtcg aaacatcgtt tcgtgttacc caatcacccg tcaatgatcc
     9061 gccgatgcga tccaacccga tcgtagataa aacgtatgcc gaaaacttca acaaagtatt
     9121 ggtacaaaaa ctatctctcg ttttcacgga tattgataat agttcactga cctatggtgt
     9181 ctccacgatc tccaaagatc aggatgtttt cggttttata agcaacgaca gcttgtatgt
     9241 attgagcagt aaagactcca acggcgtgga tagtattcgc gtcacggcta gcgacggcat
     9301 gtttaccgca gccgatacgt ttcgtgtcaa tgtaaccaat gtgaatacgg cgcctacggt
     9361 cacttccgcc atcaacaatg tgacggtcag cgaagacttc ggaaaaaact ttgtccgcct
     9421 tttaactacg gtgttttcgg atatagatgg taacgcgtta tcctacagcg tgtccgtatt
     9481 accgggggac aatgatgtaa ccgccatgat cagcaatgat tcattgtaca ttctttcctc
     9541 tgtggactca tccggcggag ataccctccg cgtcacggca agcgacggtc aatattccgt
     9601 ccagacgcaa tttacggtta cggtcaatcc tgtaaacgac ggacccaaaa ttatttcagc
     9661 cttacgtgat acgacctaca atgaggattt tccaaaaact ttagttcgcc gtttatcgtc
     9721 agtttttctg gacgccgatc atccctcatt gacgtatgcg ggatccgtat tgtcgggcgg
     9781 cgcatcggtt gttgtttcaa atgacagttt gtatgcaacc ggaacactga atgccaacgg
     9841 cacagcggcg atccgcgtaa gcgctacgga tggcacactc agcgtagcgg atacatttca
     9901 gatcaatatc aaacccgtca acgatgcacc cgtgttttct aaaaccgtcg cggataccag
     9961 tctcttggaa gatttcggaa aagtttttta tcgcaaactg agtacgcatg tctcggatgt
    10021 agacaatgct tcattaacct acaatgcgta cgtattaagc ggcagtatac aagttcagat
    10081 atcaaccgat tcgatttcac taatttccca agccgattat tacggcgcgg caaccatccg
    10141 ctggaccgca agcgacggat cgtatacact ggccgatact tttacagtca atatcgctaa
    10201 cgtaaatgac ggcccggttc gcgtaaccgc tttacgtgac accgttttca gcgaagactt
    10261 cggccatgta tttgtccgac aacttggtac ggtgttttct gatgtagaca atgcggcgct
    10321 catttatacg gcaacggtct tatcgggaaa tgcaacaaca gcaatcccat caggcaaaga
    10381 cagtttatat attcagagca gtacggattt taacggagat ctgattattc gcacaactgc
    10441 ttccgatggc gctttgtcgg tcagcgacac atttaccgtt accgtatccc cgctcaatga
    10501 cggaccttat cgtaccgtta acatttccga tatttcggta caggaagaat tttccaaaac
    10561 ctatgttgct aaacttagcc agcatttcag tgacaacgac gacgcttcgc tgagttacgc
    10621 ggtgatcaat aatccgtcat catttgatgc gatgatcggc ggcgacacgc tttacatcac
    10681 aagtcgcccg gacagtaacg gggtatatag tttggttgtc cgaaccaaag acgcctcctt
    10741 atcagcctat gatacgctta ccgttaccat taacaatgta aatgacgcgc cgtttgttgc
    10801 gacgccgttg ccggataccg atgtggatga agattttgaa aagtactttg tagccgacct
    10861 ccaacaaatt ttcagcgata tagacaatag cacgcccgcg tattcagtta cttcattgac
    10921 gccatctgta gtccaggcgc aagtttcggg tgattcgctt taccttatca gtgtacctgc
    10981 cgtatatggc tccggacaga ttcgcgtgac cgccgacgac gggcaagcct ccgcggccga
    11041 cacgatcacc gtaaccgtaa acaatgtcaa taattcgccc tatgtcgcaa atgttttgcg
    11101 cgacacggtt ttacaagaag attttggaaa agtcttttta gtaacgcttt cctcgcattt
    11161 ctcagatatt gataatgtat ctctacaata ttcggcacgt gctttacaaa acggactaaa
    11221 agcgcagatc catcaagaca gtttgtacgt gctcagtgat gcggattatt atgggatcgt
    11281 cgaggtggaa atcacagccg ccgacggata cttggcatcg cttgatacga tgcagattgt
    11341 tttgaccaat accaatgacg caccatacat ggcggcatcc gcaccggata tcgttttgaa
    11401 tgaggatttc ggcacatgga cttacggcaa cgccggaacc tattttgccg acatagacaa
    11461 tgcgttaacc tacggcgttg aagttttaag cgccggagta aatgccgctg tgtttggaga
    11521 caccataaaa ctttacagca ccgctcgctt ttatggaacc tctcaaattc gtttatttgc
    11581 gaccgacgga caattcacaa tttcagacac gatggcggta caaataaaca atgtgaataa
    11641 caccccgtat gtagcggcct ccgttccgga tacaacgata ctcgaagatt tcggttatac
    11701 ttggatacgt actcttagcc cttattttca ggactacgat aatgcaacgt tgacatttgc
    11761 tgctacagca ctaacgcccg gtgtaagtgt ggaaacaggc catgatacgc tgtacatcca
    11821 aagccaatcc gactttttcg gctcggtcgc aattaagtta tcagcgagcg atggtgaata
    11881 tacaactaca gacacaatgg ctatcaccat tcaaaatgtt aacgatagcc cggcactttc
    11941 ccatccgatg cgtgatacga gtcttgcaga agatttcggt aaaacggctt tataccaatt
    12001 gaccgacatt ttttctgata tagataatgg atctttgtcg tacggcagtg aagcactggc
    12061 agccggagtt acaggtagca taagcggtga tacactctat gtccacagca caccgtattt
    12121 caatggcgat gcggacgtac gaatttttgc aacggacgga gaatatcaag tttctgatac
    12181 gattcggatc tcggtcaccg gcgtgaataa cgcgccttat cgtcttgccg cattgccgga
    12241 cacatcactt cctgaagact ttggaaagat gccttaccgc gttgtatcgc aattcttcag
    12301 cgatgtggat aatatgacgc tcacgtattc agcgcaagtg ctccaaggcc agatcggcgt
    12361 gcaaaccgtc aacgatagtc tttatttcat atccgtacag gatttcaacg gtgccgcatc
    12421 cgtcaaagta accgcttcgg atggattttt tactgtgcat gacacattgg tattgacggt
    12481 gacgcctatg aacgatgcgc ccgcgtcggc tttacctttg cagcccggtc cgtcagtgct
    12541 gaccaataat ctgcgcccga catttaattg gcacgcctca aaagatattg acggcgatgc
    12601 gatcacctac acattaaccg ttgcgcgtga caacgctttc agcgatgtga ttttaactca
    12661 gcaaagcacc gacaccgttg cgaccgcttc ggccgacatg gatacgacag ccgtactctt
    12721 ctggcgtatc acagccaatg ataaccatgg cggcgtagtg atcggaccgg cgacaagttt
    12781 tagcaccgat gcccgcaagg ccttttttca aatcggggtt ctggccaata ctgttagccc
    12841 cctgcacgta ggtctatatg tgcgcagcac caaacagcta aacgggaacc ctgccggcca
    12901 gtttgaactt tcagaaaacg gcaatgtgat ttctgcacac aatgatacgt ttgaaaaatt
    12961 aacagcgcat accgaaccta cttattatac caactatgtg atccggcaat ccggaactct
    13021 taaaatttcc gttacaggaa cggatatggt aggtaacgtt accgtcgcac agcgtaatta
    13081 tgctgtagat gtacttgcac gcaataaacc attcgctaca gcttccgacg gcattgtttg
    13141 gtcaggcacg gcgatgtccg aaggtatgac ggctatcgca cgtgaagacg cagacgccgg
    13201 gccaatgaat aaaaatacaa gcgcctcctt gtacacggac gaagcgatca gtcccgttta
    13261 cgaaatgatc gttacggcgg atgtaaaagg caaactaacc ctcacgtaca gcgacgacgt
    13321 gatttcgaac ttacatcgta ttatttccgg tgaagtggat gagcgattga tcgggattta
    13381 tgctgaagaa aacggacaat gggtttatat cggcggtcaa agggaaa
//
LOCUS       DAOQNQ010000059        13197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1663_length_13197_cov_5.898189, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000059 DAOQNQ010000000
VERSION     DAOQNQ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13197
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1663_length_13197_cov_5.898189"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2492
                     /locus_tag="PK754_05650"
     CDS             <1..2492
                     /locus_tag="PK754_05650"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNB09020.1"
                     /translation="WAGALGDYDNDGLLDIIIAAPLGFEGISSSNLLFHNDGNAQFTQ
                     IDTSVITLQTGPFTVPSWYDYDMDGDIDLFIGSGPATGNAGPDFYYKNMLNETGTAYF
                     RRMTDNFASELRDGQNVNWFDFDNDGDFDFFVTNYGFGNNSIQPNHFYRNDAGTFTKI
                     STGAISSDADVSLGNLWEDFDNDGDLDCFISNENGDSSRYYQNNNDGTFTRIDTLAMS
                     NGTNPRRGATAGDFDQDGDLDLYVYSSFTGVNNFFRNDLTNGNHWINFRLSGKVTNHT
                     AWGAIIRVKATIGGNSRWQTRQMSAQNSFSGHSAELQHFGMADATNVDSVEIRWPSGI
                     RQYVTSLAVNQTVDIIEDTSLSYKPNFKWLSNNTTFVDSSYTTTLFAHANPKAVYRLI
                     QSPSGMTIDSISGLIQWMPNATQTGNQTVRVSAVNSQGTQERQYTLNVIQLVKPQIIP
                     LPDRATMVGMNVSVFAKYVTTPTQPATYQLVQGPAGMTINSIAGHITWKPTSSQLGSH
                     TIKIRSVNSVGSDSITFTIDVKEQPKIYVLQNPVATHTVDLITISEISLRSKPFIQVT
                     QRVFAPGQGPFVVSDSVSPGVYRGSWVFDSTRTYQINATFSDSNGFTGSLQKIFTVAI
                     IAADKPTLIQTMDSRFNVFVPARAIASERFVIIESSQEAGMDFINFGNALELQSQAHI
                     ELNAWRNPKIEFETINGWEEIPVERVGRLYRANIKHLGKFRISESTDSGISDKVPHNL
                     SLSQNYPNPFNPTTTIRFDLHQTSDIRLTVYNTLGQRIRLVTQGVFSAGTYRVIWDGR
                     NDQGAAVASGIYIYRLESDGNFFSKRMMLIK"
     gene            2508..3095
                     /locus_tag="PK754_05655"
     CDS             2508..3095
                     /locus_tag="PK754_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09021.1"
                     /translation="MKKLTYIFLTGVFYLLGCGGGRDSADPIQKGWSAFESGEFYESR
                     DIFLNIYLDNPNDAEAAHGLSWSLLRMDSVSRAVTMFESSEGALPPEDVTGGYTFALS
                     AKKMYNESNTQADILINASPTWTFTHGSEINVIDIYIVKSANYFALGNYPASLNSIKM
                     VNASFSADVTTASGRSSLAAEIERLRGLNKVNISE"
     gene            complement(3177..4556)
                     /locus_tag="PK754_05660"
     CDS             complement(3177..4556)
                     /locus_tag="PK754_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNB09022.1"
                     /translation="MKSAKYILILAAMFISVAFAQKETPPKGSAPKDFSLPKRETISL
                     PNGLQASLIRYGNVPKAMIRVVVRTGNIDEKNNETWLADLTGEFLSEGTASKNAEAVA
                     TAAANMGGSLNINTGLDQSFVAGEVLSEFTPDFIKLSADVIRNPAFPASELERLKSKM
                     LRDLSVQSSTPQSQALEKYSKVMYGDHAYGRTFPTEAMLKSYTLDQVKSFYVANFSAK
                     RTHIFVVGVFDPAAVTKAIREAFGDWKEGTPPTQNVPQAKSERTIYLIDRPGAPQSTI
                     ILGLPTMDASHPDYVKFLIMNSILGGSFGSRITTNIRENKGYTYSPFSAITSRYHSAN
                     WSEQADVTTEHTGASIKEILYEIERLKKEPPAKDELEGIQNYQAGIFVLQNSNFNGIV
                     NQLAFLNLHGLPDTYLSNYVKNVYAVTPEDVSNMAKSQLKDDAYTIVIVGDIAKIEKQ
                     VKLYGKIVK"
     gene            complement(4553..5950)
                     /locus_tag="PK754_05665"
     CDS             complement(4553..5950)
                     /locus_tag="PK754_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNB09023.1"
                     /translation="MKPGLRLLSLGLSILLAISSCAKKEDAVPKFSVPVEYHKLENGL
                     KVVLSPDATAPTATVAVYYNIGFRIEPKDRTGFAHLFEHMMFQGSQNLGKMEFIQLIQ
                     RNGGILNGSTRFDFTNYFQVVPSNKLETILWGEADRMKGLAITKENLKNQQEVVKNEV
                     KVNVLNQPYGGFPWLDMPQYANTNWYNAHNFYGDLKDLDAATLEDVQSFFKTYYAPNN
                     AALVVVGDFEPKQTLEWIKKYFGSIAAQPQPAKPDISEPEQTGEKKSKKQDPLATRPA
                     VAVAYQMPARNTPEYYAMGLLDQILVQGEDSKLHQALVQKKGYTGQVSGGINYLGNMF
                     NYNGPMLWMMDLIHDQNVSADSIMLEVDRIMQELAEKPVDQATLNRALVKMRSSLYGE
                     FNQTGGLGKMDLLACFALFDDNPARINTLEDEFRKVTPELIRETAKKYLRSTNRTILS
                     VEATNVMKNKMGAAQ"
     gene            6172..7254
                     /locus_tag="PK754_05670"
     CDS             6172..7254
                     /locus_tag="PK754_05670"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNB09024.1"
                     /translation="MQKETGILDTLSNDVEKADFWHTMKREYRELHEFYIDSDRKKRL
                     LSMGRFKRAMHIAAWILQGMFYKLTPARRLLIVAGILLSINGITFKFASWLVGDRGLL
                     GALLIFFVLMLELKDKLLAHDELDAGRTIQRALLPPEQPNIPGWSVWLFTRPAREVGG
                     DLVDIIDHDSKTMSILMADVSGKGLRAALWTAKLQATIRALITMITDPLDLVDRVNKI
                     FHRDSLRSVFASMVLLRVNKSSGQIQYVNAGHLPPVLCNRDGKTECGRGQLAIGLSPS
                     SKYVLNELTMAIGDVLVSFSDGVTEAANENGEFYGNDKPLQIAERLRNTDAPVIGKAI
                     LLDLERFVGESSASDDISIIILKRHS"
     gene            complement(7270..7698)
                     /locus_tag="PK754_05675"
     CDS             complement(7270..7698)
                     /locus_tag="PK754_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09025.1"
                     /translation="MNKTINIFIIVVLHTAAHVLVAQNDEDLVSFIIGQYEIIGRYPG
                     SKKPFIGSAVVTLDNGKIFVKRTIDKKTEMADAAFIKVTADQITVLQCRFQSGKETYV
                     GVYMIKGDLDNYPRLTGQLFKSKDESFPSGIEAWFPIIPN"
     gene            7903..8628
                     /locus_tag="PK754_05680"
     CDS             7903..8628
                     /locus_tag="PK754_05680"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNB09026.1"
                     /translation="MEKVMQSDRGFKSVLAEVYQKNMVKGLSAGVVLHAFIIGTIVWV
                     IGQNPKAVKQVMNQISILVDMPPLTNIPEIPAINLPQKFNPSDIIKGIPIIAPDAIVS
                     PDITIPAQDDWIEKFSDNSVPMDWKNLDVSLPNFENVSIDSKNTSSGEFTIVEQMPEV
                     IHRVKPEYPSLPRQAGIEGRVYVKGLVNEEGMVTRTVFVSGDEMFYNAASDAFLKTRF
                     KPGINGGVAVKVWIMMPFTFRLR"
     gene            complement(8673..11303)
                     /gene="clpB"
                     /locus_tag="PK754_05685"
     CDS             complement(8673..11303)
                     /gene="clpB"
                     /locus_tag="PK754_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HNB09027.1"
                     /translation="MNSDKMTIKAQEALSKAQQLALERHHTQIEPEHILMAMLDDHDG
                     TVPALIKKLGSPLATIRTQAESILDRMPRVSDTAQQVYLSGRASRILIEAGKEAEKLK
                     DEYISTEHLLIAMAEESTESGKMLKGFGIWRETLYRALRDVRGAQRVTDQNPEGKYQA
                     LQKYGRNLNELARRSKLDPVIGRDEEIRRVLQVLSRRTKNNPVLIGEPGVGKTAIAEG
                     LAHRIIDGDVPENLRTKTVMALDMGALIAGAKFRGEFEERLKAVLREVTESQGEIILF
                     IDELHTLVGAGAAEGAVDAANMLKPALARGELRAIGATTLDEYRKHIEKDPALERRFQ
                     PVLVDEPSVHDTISILRGLKEKYEVHHGVRIQDNAIIAAAVLSDRYIADRFMPDKAID
                     LIDEAASKLRIEINSMPEELDTVERTIKQLEIEQLALKKENDTVSLSRLDKISSELAD
                     LKEKRTALRSHWETEKKVIEQIRRVQSEIEITKSQIEQSERAGDLNKAAELKYGRLTE
                     LGTQMEKEKNKLAEVQKNQKMLKEEVSEDDIAEIVAKWTGIPVSRMLESEKDKILSMP
                     ERLQERVVGQNEAVVSVSDAIKRSRAGLSDEKRPIGSFIFLGPTGVGKTELAKAVAEF
                     LFDDETAMVRLDMSEYMEQHSVSRLIGAPPGYIGYDEGGQLTEAIRKKPYSVILLDEI
                     EKAHPEVFNTLLQVLDDGHLTDNKGRTVNFRNTIIIMTSNLGASVIMEKMNDMASENR
                     DRIHARIKESVISLLRQRLRPEFINRIDDIVVFRPLERNDLQRIVHIQFAHVKKLLQK
                     HNIGIELDTTAVDALARLGFDPIFGARPLKRLIQKDVVTQISTELLKGAFVPNDQLLL
                     AFHDDAFRLTKRNNSLKSVA"
     gene            complement(11377..11526)
                     /locus_tag="PK754_05690"
     CDS             complement(11377..11526)
                     /locus_tag="PK754_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09028.1"
                     /translation="MHVVRSITCGGMRVPTIPHLFTEQNAMAYGLKISVILMICLALY
                     MIMTA"
     gene            complement(11545..11994)
                     /locus_tag="PK754_05695"
     CDS             complement(11545..11994)
                     /locus_tag="PK754_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNB09029.1"
                     /translation="MSLIKFTPRDRDIFSDLFAIQREMNQAFNSVFNRDEYTGLTNWN
                     PATDIVEGKDEYTVRIELPGVSKNDVKITIHDNVLTVQGEKKQESENKENNYHRVERS
                     YGSFMRSFRLPSMVKGEKIDANYKDGILTIVLPKAEEAKTKEIEIKF"
     gene            complement(12022..12408)
                     /locus_tag="PK754_05700"
     CDS             complement(12022..12408)
                     /locus_tag="PK754_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNB09030.1"
                     /translation="METKQEYEVKTQAIAKPENNYYYIQPRTDIRENKDNYLLYVEMP
                     GVRKDGLTVTLQNGNLVIEGKMGLNEEGSVIREEIPKRNYHRVFRLTNRVDPNKIEAR
                     WQDGFLILSVGKKEELKPREIAINFN"
     gene            12818..>13197
                     /locus_tag="PK754_05705"
     CDS             12818..>13197
                     /locus_tag="PK754_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNB09031.1"
                     /translation="MNEEFQIRLDIVECGRRMYARNYVASNDGNISVRVGDRIIATPT
                     GISKGFMRAEDMVVLDMQGRVIEGARKPSTEIKMHLAIYDERKDVNSVVHAHPVHATG
                     FATAQIELKDCVLAEIVTTLGSIP"
BASE COUNT         3546 a         2691 c         2975 g         3985 t
ORIGIN      
        1 gttgggccgg cgctttgggc gattatgata atgacggctt attggatatt atcattgctg
       61 cgccgcttgg ttttgaaggt atttccagta gcaatcttct ttttcataat gacggaaacg
      121 ctcaattcac gcaaattgac acatccgtga ttacgttaca aacgggtccg tttaccgttc
      181 ccagttggta tgattatgat atggacggag atattgattt gtttattgga agcggtccgg
      241 caaccggaaa cgccggtccc gacttttatt ataaaaacat gcttaatgaa acaggaacgg
      301 catatttcag aagaatgacc gataattttg ctagcgaact tcgagacggt caaaatgtaa
      361 actggtttga ttttgacaat gacggtgatt ttgatttttt tgtaacaaat tatgggttcg
      421 gaaataattc aattcaaccg aatcattttt atagaaatga tgcgggtacg ttcacaaaaa
      481 tatctaccgg cgctatttca tccgatgcgg acgtatcctt gggcaatctt tgggaagact
      541 ttgataatga cggggacttg gactgcttta tttcaaatga aaacggagat agcagccgat
      601 attatcaaaa taataacgac gggactttta cccgcattga tacactggca atgtccaacg
      661 gaaccaatcc acgacgcggc gctacagccg gagattttga tcaagacggg gatttagatt
      721 tgtatgttta tagttctttt acgggggtta acaatttctt tcgcaatgat ctgacgaatg
      781 gaaatcattg gattaatttt cgcttatccg gcaaagtaac caaccataca gcttggggcg
      841 ccattattcg cgtcaaagca acaatcggag gcaattctcg atggcaaacc cgccaaatgt
      901 cggcacaaaa ttcgttcagc ggtcatagtg cggaattaca gcatttcggt atggctgatg
      961 ctacgaatgt tgattcggtt gaaatacgtt ggccatccgg gatacggcaa tacgttacct
     1021 cattagctgt taaccaaacg gtagatatca tcgaagacac gagtttgagt tataaaccta
     1081 attttaaatg gctatccaac aatactacat ttgttgattc atcatataca accacgttat
     1141 ttgctcatgc caatccaaaa gccgtttata ggctcattca gtcaccctca ggtatgacga
     1201 ttgattccat tagcggttta atacagtgga tgcccaatgc tacacaaacc ggtaatcaaa
     1261 cggtgcgtgt atcggctgtt aattctcagg gtactcagga aaggcaatac acgcttaatg
     1321 taatccagtt ggtaaaacct caaatcattc cactgcctga tcgtgcgacg atggtgggaa
     1381 tgaatgtaag tgtttttgca aaatatgtta ccacacctac ccaaccggct acctatcaac
     1441 tggtacaagg gccagcgggg atgactatca atagcattgc aggacatatt acatggaaac
     1501 cgacttcgtc gcaactcgga tcgcatacta taaaaatcag atcggttaat tcagtcggtt
     1561 ctgattcgat aacatttact atcgatgtca aagagcaacc gaaaatttac gttctacaaa
     1621 acccggttgc tacccatacc gttgatttga ttactatcag tgaaatcagc ttacgatcaa
     1681 aaccgtttat acaggttacg caaagggttt tcgcacccgg gcaaggtccc tttgttgtat
     1741 ccgactcagt gtcaccgggt gtttatcgcg gatcatgggt tttcgattcg acacggacat
     1801 accaaatcaa tgcgacattt tcagattcca atggtttcac aggaagtctt caaaaaatat
     1861 tcacagtagc gatcatcgcc gcagataaac ctacattgat tcaaacgatg gatagccggt
     1921 ttaacgtctt tgtcccggcg cgcgccatag cctctgaacg attcgttatt atcgaatcgt
     1981 cacaagaagc gggtatggat tttataaatt ttggaaatgc tctcgaattg caatcacagg
     2041 cacacataga gttgaatgca tggcgcaatc ctaaaataga atttgaaaca attaatggtt
     2101 gggaagagat tccggtcgaa agagtgggtc gtttgtatag agctaatatc aaacatctag
     2161 gaaaatttcg aatttcggaa tctacagact ctggaatttc tgacaaagta ccacataatt
     2221 tgagtttatc tcaaaattat ccgaatccat ttaatcctac gacgacaata cgatttgacc
     2281 ttcatcagac gtcagatata cgattgacgg tttataatac gcttgggcag agaatacgtt
     2341 tggtgacaca aggtgttttt tccgccggta cgtatcgtgt tatatgggac gggcgcaatg
     2401 atcaaggtgc agccgttgca agcggtatct atatttatcg tctcgaaagc gatggtaatt
     2461 ttttttccaa acgaatgatg ctaatcaaat aaactaaggt ataatctatg aaaaagttga
     2521 catatatatt tttaacagga gtattttatt tattgggatg cggcggcgga cgtgattctg
     2581 ccgatcccat tcaaaagggt tggtcggctt ttgagtcagg cgaattttat gaatcacgcg
     2641 atattttttt gaatatttat ttggataatc ccaacgatgc ggaagcagcg cacggtttga
     2701 gttggtctct tttgcgtatg gattcggtta gccgagctgt taccatgttt gagtcatcag
     2761 aaggagcatt gcctccagaa gatgtgacgg gcggttatac atttgcactg agcgcaaaaa
     2821 agatgtataa cgaatccaat acacaagcgg atatattgat caacgcctct ccgacgtgga
     2881 cgtttaccca tgggtccgag attaatgtta tagatatcta catagttaaa tcggctaatt
     2941 attttgcgct tggtaattat ccggcctcgt taaacagcat aaaaatggtc aatgcgtctt
     3001 ttagcgctga tgtaactacg gcttcaggtc gctcttcact ggccgcagaa attgagcgat
     3061 tacgaggatt gaataaagta aatattagtg aataagttga ttgaattcag ttttcaagtt
     3121 tagaaataga aaaagcacgc cgcaagcgtg ctttttctat aggataatac aacgtattat
     3181 ttcacgatct ttccgtacag ttttacctgt ttttcgattt tagcaatgtc tccgacaatg
     3241 acgatcgtat aagcatcgtc ctttaattga gacttagcca tgttggatac atcctccggt
     3301 gtcacggcat atacattttt aacataatta ctaagatacg tatccggtaa accgtgcaaa
     3361 ttgaggaaag ctaattgatt gacgataccg ttgaagtttg aattttgtaa tacgaaaatc
     3421 ccggcctgat aattttgaat tccttccaat tcatctttag ccggaggttc ttttttaagg
     3481 cgctcgattt cgtataaaat ttctttgata gaggcaccgg tatgctcggt tgtcacgtca
     3541 gcctgttccg accagttagc gctatgatat cgtgaagtga tagcgctgaa aggcgaatac
     3601 gtgtagcctt tgttttcgcg gatattagtg gtaatacgcg aaccgaatga tcctcccaaa
     3661 atggagttca ttatgagaaa tttgacataa tccggatggg aagcatccat cgtaggtaaa
     3721 ccgagtatta tcgtagattg cggtgcgccg gggcggtcaa tcagataaat tgttcgttcg
     3781 gacttagcct gaggtacgtt ttgtgtcggc ggggtccctt ctttccagtc gccaaaggct
     3841 tcacgtatgg ctttagttac agcagcaggg tcaaatacgc ccacaacaaa aatatgagtg
     3901 cgtttagctg aaaaattggc cacataaaat gattttacct gatcaagggt ataactcttg
     3961 agcatggctt cggtaggaaa agtacgtcca tacgcgtgat cgccatacat cactttgctg
     4021 tatttttcca gtgcttggga ttgcggtgta ctgctttgta cgctcagatc acgaagcatt
     4081 ttggatttta gacgctcaag ttcagaagca gggaaagcgg gatttcgaat cacgtcagcc
     4141 gaaagtttga tgaaatccgg agtgaattcc gataagactt caccggctac aaatgattgg
     4201 tcaagcccgg tattgatatt taatgatccg cccatattgg ctgcagctgt tgccacggct
     4261 tcggcatttt ttgatgctgt gccttcactc aaaaactcac cggtcaaatc agcaagccac
     4321 gtttcgttgt ttttttcatc aatattaccg gtacgcacaa cgacgcgtat catggctttg
     4381 ggtacattgc cgtagcgaat gagcgaagct tgtaaaccat tgggcagaga aatagtttca
     4441 cgttttggca gtgaaaagtc ttttggcgct gaacctttag ggggcgtttc tttttgggca
     4501 aaggccacag aaataaacat ggcggccagt attagaatat attttgcgga tttcattgcg
     4561 cggctcccat tttatttttc atcacattgg ttgcttctac cgaaagtatc gtccgattgg
     4621 ttgaacgcag atatttcttg gctgtttcgc gaataagctc cggcgtgact ttacggaact
     4681 cgtcttccag cgtattgatt cgtgccgggt tatcatcaaa taaggcaaag caggcaagta
     4741 ggtccatttt gcccaatccg ccggtttgat taaattcgcc atacagcgat gaacgcattt
     4801 tgactaaggc acggttgagt gtagcctgat caacaggttt ttctgcgagt tcctgcataa
     4861 tacgatcaac ttcgagcatg atgctgtcgg ccgaaacatt ctgatcgtga ataagatcca
     4921 tcatccaaag catcggcccg ttatagttaa acatattacc aagatagttt ataccaccgc
     4981 ttacctggcc cgtatatcct tttttctgaa ctaaagcttg gtgtaatttg ctgtcttcgc
     5041 cctgcacaag gatttgatcc aacaatccca tggcataata ctcaggcgta ttgcgagcag
     5101 gcatctggta ggccacggct actgccggac gagtcgcaag tggatcctgt tttttggatt
     5161 ttttctctcc agtttgctcc ggttcgctga tatccggttt cgccggctgg ggttgagccg
     5221 cgatactgcc aaaatatttc tttatccatt ccaacgtttg tttgggttca aaatcaccga
     5281 ccaccaccaa cgcggcattg ttgggtgcat aatatgtttt gaaaaatgac tgaacatctt
     5341 caagcgtagc ggcatccaga tctttaaggt cgccgtaaaa attatgtgca ttataccaat
     5401 tggtattggc atactgtggc atatcaagcc aagggaatcc gccataaggc tgattgagaa
     5461 cattgacttt tacttcgttt ttgacaactt cctgttggtt tttaagattt tctttggtaa
     5521 tagcgaggcc tttcatacgg tcggcttcgc cccacagaat ggtttccaat ttattagacg
     5581 gcacaacttg aaaataatta gtaaaatcaa aacgggtcga gccattaaga ataccgccgt
     5641 tacgttgaat caattggata aattccattt taccgagatt ctgtgaacct tggaacatca
     5701 tatgttcgaa taggtgtgcg aatcccgttc gatctttggg ttcgatgcga aagccgatat
     5761 tgtaatacac ggcaacagtc gccgtcgggg ctgtggcatc cggtgaaagt acgactttca
     5821 aaccgttttc aagtttgtgg tattctaccg gaacactaaa tttgggaaca gcatcttctt
     5881 ttttggcgca tgatgatatc gccaacagaa tgcttagtcc caggcttaac aaacgcaatc
     5941 ctggtttcat cggaactcct ccatgggttt atgagatcgg aatagtttca aaagtaaatt
     6001 attactttgt cagaaatgaa tcgtaacaag gctaattcgt aatcggttac ggataaatta
     6061 taaataagtt tcaatttatg ttagtataat tattccgttt acttgttggt gtgatttaca
     6121 aacatttgcg ggttatcttc gtataaaaaa aataataaca gattgaagat tatgcaaaaa
     6181 gaaacgggta tactggatac gttgagtaac gatgtcgaaa aggctgattt ttggcatacg
     6241 atgaaacggg aataccgcga actgcacgag ttttacattg actcggatcg aaaaaagcgg
     6301 ttgttatcca tgggtcgctt caaacgtgcc atgcacatcg ctgcgtggat attgcaaggt
     6361 atgttttaca aacttactcc cgcccgacga ctattgatcg tagccgggat tttgctgagt
     6421 atcaatggga ttacatttaa gtttgcatca tggcttgtgg gagatcgggg gcttttgggt
     6481 gcgttactta tcttttttgt cctcatgttg gaactaaagg ataaattgct cgcgcatgac
     6541 gaactggatg ccggacgaac gattcagcgt gcgcttttac ctccggagca gcctaatata
     6601 cccgggtggt ccgtttggtt atttaccagg ccggcacgcg aagtaggtgg agatttggtg
     6661 gatattatag atcatgacag caagacaatg agtattctga tggccgatgt ttcaggaaag
     6721 ggattgcgtg ctgctttgtg gacggccaaa ttgcaagcca cgattcgtgc actgatcact
     6781 atgataaccg atcctttgga tttggtggat cgggttaata agatatttca tcgtgacagc
     6841 ctgcgtagtg tatttgcctc catggtgctt ttgcgtgtca ataaatcttc ggggcagata
     6901 caatatgtca atgccggaca tttaccccct gttttgtgca atcgtgatgg gaaaacggag
     6961 tgcggacgcg ggcaattggc gatcggactt tcaccgtcat caaaatacgt tttaaacgag
     7021 ctaacgatgg cgattgggga tgtgctcgtc tcgttttcgg acggtgtgac cgaagcggct
     7081 aatgaaaatg gagaatttta tggaaatgat aaaccgctac aaatcgccga gcgattaaga
     7141 aatacggatg caccggttat cgggaaagcc attttgcttg atctggaacg ctttgtgggt
     7201 gaatcttccg cttccgatga tatttcgata attattttaa aacgtcacag ttgaaaagat
     7261 aataacgaac taatttggta tgatcgggaa ccacgcttca atacctgaag gaaatgactc
     7321 gtccttgctt ttaaaaagct gtccggttag ccgtgggtag ttgtccaaat cacctttgat
     7381 catataaacc ccgacatatg tttcttttcc cgattggaac cggcattgga gaacggttat
     7441 ttggtcggct gtcaccttta taaatgcggc atcagccatt tcagtttttt tatctatcgt
     7501 acgttttaca aatatctttc cgttatccaa agtcaccaca gccgaaccga taaatggttt
     7561 tttactgccg ggatatcgac cgataatctc atattgacca attataaaag aaacgagatc
     7621 ttcatcattc tgtgcaacca aaacatgagc tgcggtatgc aaaacaacga taataaagat
     7681 atttatagtt ttattcataa aaccctttcg atgtgtatat ttaatccatg aatagccaaa
     7741 attaaatcgc aattattgta ataacaacat attcagacag ttcttaccga ttatcaccat
     7801 tctatagttg cgatatgaag acatatcgtt ccgctatttt atatgtagat acttgttttg
     7861 gatcattaag ctcaagcaat ctactatgaa aaggagtcgg ttatggaaaa ggtaatgcaa
     7921 tctgaccgtg gattcaaatc cgtgttggct gaagtttacc aaaaaaatat ggtaaaagga
     7981 ttaagtgcgg gtgttgtttt acatgctttc attatcggaa cgattgtgtg ggtaatcggc
     8041 caaaacccaa aagcagttaa gcaggtcatg aaccaaatat ctatactggt ggatatgcca
     8101 cctttaacaa acattccgga aattcctgcg atcaatttac ctcaaaaatt taatccgagc
     8161 gacattatca aaggaatccc tatcatagct cctgatgcca tagtttctcc cgatattacg
     8221 atacctgcgc aagacgactg gattgaaaag ttctctgata atagtgttcc gatggattgg
     8281 aagaatcttg atgtgagctt accgaatttt gaaaatgtga gtatcgatag taaaaacaca
     8341 agttcaggcg aatttacgat agtggagcag atgcccgagg tgatacatcg cgtgaagccc
     8401 gaatacccat ctcttccaag acaagccggc attgaaggta gagtatatgt taaggggtta
     8461 gtcaacgaag aaggtatggt aacccgcacg gtttttgttt caggggatga gatgttttat
     8521 aacgcggctt cagatgcctt tttgaaaaca cgtttcaaac cgggaatcaa tggcggcgta
     8581 gccgtaaaag tatggattat gatgcccttt acttttcgtt tgcgttagat caaagggtgt
     8641 ttctgtaagt tcagagacac ccttttgatg atttaggcaa cagattttag cgaattattg
     8701 cgtttggtta atcggaaagc gtcatcatgg aacgcaagta atagttgatc atttggaaca
     8761 aaagctcctt tgagaagctc tgtggatatc tgagttacga cgtccttttg gatcaaccgt
     8821 tttagcggac gggcgccaaa tatcggatca aatccgagcc gggctaatgc atccaccgcc
     8881 gtggtgtcca attcgatccc tatgttatgt ttttgtagaa gtttttttac atgagcaaat
     8941 tgtatatgta caattcgctg caaatcattg cgttccaacg gcctgaatac gacgatatcg
     9001 tcaatacggt tgataaactc cgggcgtaaa cgttggcgga gcagggatat gacggattct
     9061 ttgatacggg cgtgaatgcg atcacggttt tcggacgcca tatcattcat tttttccatg
     9121 attacagaag cgccaagatt ggatgtcatg ataatgatgg tattacggaa attaacggta
     9181 cggcctttat tatctgtaag atgcccgtca tccaatactt gaagtaaggt attaaaaact
     9241 tcgggatgtg ctttttctat ttcatcaagc agaataacac tatacggttt tttgcgaatg
     9301 gcttccgtca actgaccacc ttcgtcgtag ccgatatagc ccggaggcgc accgatgagg
     9361 cgtgaaacgc tgtgttgttc catgtattcg gacatatcca gtcgtaccat agcagtttca
     9421 tcgtcaaata aaaattcggc aacagctttg gctaattcag tttttccaac accggtcggg
     9481 cccaagaaaa tgaatgagcc gatgggtctt ttttcatccg ataatcctgc acgcgatcgt
     9541 ttgatcgcat ccgaaacaga tacaacggct tcgttttgac cgacaacgcg ttcttgaagg
     9601 cgctccggca tgcttaaaat tttatctttc tctgattcta acatgcgaga gacggggatg
     9661 ccggtccact tggcaacaat ctcggcaata tcatcttcac tgacctcttc tttgagcatt
     9721 ttttggtttt tctgaacttc ggctaattta tttttttcct tttccatttg tgtaccgagt
     9781 tcagtgagcc gtccgtattt gagttccgcg gctttattga gatcaccggc gcgttcggat
     9841 tgttcgatct gcgattttgt aatttctatt tcagattgga cgcggcgaat ttgttcgata
     9901 acttttttct ccgtttccca atgactacgc agcgccgtac gtttttcttt taggtcagcg
     9961 agttcgcttg aaattttatc cagtcggctt agggatacgg tgtcgttttc ttttttaagc
    10021 gccaattgtt cgatttccag ttgtttgatg gtacgctcaa ccgtatccaa ctcttcaggc
    10081 atcgaattga tctcaatacg tagcttggaa gccgcttcgt cgataagatc aatggctttg
    10141 tccggcataa aacgatcggc tatgtaacga tcagaaagta cggctgccgc tatgatagca
    10201 ttatcttgta tgcgaacgcc atggtgcact tcgtattttt ctttgagacc gcgtaatata
    10261 gagatggtat cgtgaacaga cggttcatcc actaaaaccg gttggaaccg ccgttcgagc
    10321 gccggatctt tttcgatgtg cttgcggtat tcatccagtg tcgtagctcc gattgcacgt
    10381 aattcgccac gcgccaatgc cggtttaagc atattagctg catccacagc gccttcggcc
    10441 gcccctgcac cgacgagtgt atgcagttcg tcaataaaaa gaataatttc accttgggat
    10501 tcggtgacct cgcgaagtac ggctttgagt cgttcttcaa attcaccgcg aaatttggcg
    10561 ccggcgatga gtgcgcccat atctaaagcc atgacggttt ttgttctgag attttcggga
    10621 acgtcgccgt caataatgcg atgggcaagg ccttcggcga tggcggtttt accgacaccg
    10681 ggttcaccga tcagtacggg attatttttg gtgcgtcgcg acaaaacctg taaaactcga
    10741 cgaatttctt cgtcgcgtcc gatgaccggg tccagtttgc tgcgtcgggc taattcattc
    10801 agattgcgcc cgtatttttg caaagcctga tatttacctt cgggattttg gtcggttacg
    10861 cgctgcgcgc cgcggacatc tcgcaatgca cgataaagtg tctcacgcca tatgccgaaa
    10921 cctttgagca tttttccgga ttccgtgctt tcctcagcca tagcaatgag aagatgttcg
    10981 gtgctgatgt attcgtcttt gagtttttcc gcttctttac cggcttcgat caatatacgg
    11041 ctcgcacgac ctgacaaata aacctgttgt gcggtatcgg aaacacgcgg catgcgatca
    11101 agtatggatt cggcctgcgt gcgtattgta gcaagcgggg agcctaattt tttgatcaag
    11161 gcaggaaccg taccgtcatg atcgtccaac atcgccatta aaatatgctc aggttcgatc
    11221 tgcgtatgat gccgctccaa tgctaactgc tgggccttag ataaagcctc ttgcgctttg
    11281 attgtcattt tatcggaatt catgagttac gttccttttc attgccttac tgcgagtgcg
    11341 gcgtattcgt gcgatttatt tgttttttgc caatgatcaa gctgtcatga tcatatacaa
    11401 tgccagacaa atcataagaa tgacacttat tttaagtccg taggccatcg cattttgctc
    11461 tgtaaataaa tgcgggatgg ttggtacacg catcccgccg caagttatac ttctgacgac
    11521 gtgcatgcga cacgccgaat tattttagaa tttaatttca atttctttcg ttttggcttc
    11581 ttcggccttg ggcaatacga tggtcaggat gccgtcttta taattggcgt caattttttc
    11641 tcccttgacc atggacggga gtcggaaaga gcgcatgaaa gagccgtagc tgcgttcgac
    11701 acgatggtaa ttattttctt tattttccga ttcctgcttt ttttcacctt ggacagtcag
    11761 cacattatcg tgaatcgtta ttttcacatc atttttgctg acaccaggta attcgatacg
    11821 aacagtatat tcgtctttgc cttcaacgat atccgttgcc gggttccaat tggttaggcc
    11881 ggtatattcg tctcgattaa agacggaatt aaaagcctgg ttcatttctc tttggatcgc
    11941 aaacaaatca gagaagatgt cgcgatcgcg cggggtgaat ttgatgagtg acatggtcaa
    12001 tctcctttgg ttgtttggtg tttagttaaa gttaattgcg atttcacgcg gcttcagttc
    12061 ttctttttta ccaacgctca ggatcagaaa tccatcttgc caacgggctt caatcttatt
    12121 gggatcaacg cggtttgtca gtcggaatac gcggtgatag ttgcgtttgg gtatttcctc
    12181 gcggataacg gatccttctt catttaagcc cattttgcct tcgatcacaa gattgccatt
    12241 ttgcaaagtg acggtgagtc catccttacg tactccgggc atttccacat ataacagata
    12301 gttgtctttg ttttcgcgaa tatccgtacg cggttgaatg tagtagtaat tattttccgg
    12361 cttggctatt gcttgtgttt tcacttcata ttcctgtttg gtttccataa gtgctcctgc
    12421 gttttagtat gttggatatt ttttgtgctc ggttaaagga attacaaacc caatgccaaa
    12481 aatatcaaaa ttaaaaaaaa caaaatttta ttaacagcgc ttatagccga cacaataatt
    12541 ttaacgacaa attgtcattg tttttagttt ccgatacgaa ccaaatgtca ttttttatga
    12601 caaaatgact gctgtcgtta attgatttag aatgagtgaa ttttttacat cagaaaaagg
    12661 agagcatgat taaaaatgaa tgccttaatc gaaaatgaag cagagtaaat tttatggagt
    12721 cgatttttga ggtatttaca ggagcgtgat acaatcacag aagttcatct tgtctttggt
    12781 ttataaaaga aatattatta ggccaatatt gaaaaatatg aacgaagaat ttcaaatacg
    12841 tttagatatc gtcgaatgcg ggcgcaggat gtatgcacgt aattatgttg catccaatga
    12901 cggcaatatc agtgtgcgag tcggtgatag gatcatcgca acccccacag gcataagcaa
    12961 ggggtttatg cgtgcggagg atatggttgt tttggatatg caaggccgcg ttatcgaagg
    13021 ggcgcgcaaa ccctcgaccg aaatcaaaat gcatctagcg atttacgacg agcggaagga
    13081 tgtaaattcc gtggtgcatg cgcatcctgt tcatgcgaca ggttttgcaa cggcgcagat
    13141 cgaactcaaa gattgtgtat tggcggaaat cgtaacgacg ttgggttcga taccttt
//
LOCUS       DAOQNQ010000060        13138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1675_length_13138_cov_7.462967, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000060 DAOQNQ010000000
VERSION     DAOQNQ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13138
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1675_length_13138_cov_7.462967"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PK754_05710"
     CDS             <1..340
                     /locus_tag="PK754_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09032.1"
                     /translation="RQIVERSDVRIDSTLSRLSSSTKIFKNILSRTDAQIDSTFGNVR
                     LITQSVMDVTQSLDSIVSKVQSGEGNLGRMIYDDQLYWRINRTTQQLDSLVGSLREKG
                     VKVNVKLFGD"
     gene            356..1090
                     /locus_tag="PK754_05715"
     CDS             356..1090
                     /locus_tag="PK754_05715"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNB09033.1"
                     /translation="MNFLLSIMLSLFAGVLIIAGGWVFSSKKNWESRYLDFFIALGAG
                     YILGVALLDMIPEAFHATPHAMYYVVLGYLIVHLFEHVLTPHFHFGEETHNHLISNVV
                     STSALIGLMVHNFFSGVAIGSGMLTSEKVGWMIFAGTVLHKLPEGFTIASIMFASHHR
                     RTYGVVGTILLALASLLGTVMIYLFEMESLPVKDVALAMSAGTFIHVATTDLIPRIND
                     SGSRRMPLVVFLGVGLFLISYMLMHP"
     gene            1184..2713
                     /locus_tag="PK754_05720"
     CDS             1184..2713
                     /locus_tag="PK754_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HNB09034.1"
                     /translation="MLKKIFSRDTLQKVLNFIERVGNLLPHPATLFGLFALTIVIVSG
                     IFSAMDISVQHPGTGEVIRPVSLLTVEGMHRILSNLVTNFTGFVPLGTVLVAMLGIGI
                     AEGSGLIGSVLRLLVISAPKRLLTFVIVFAGVMSNVAGEVGYVLLIPLAAVIFLAVGR
                     HPIAGLAAAFAGVSGGYSANLLLGTVDPLLAGLTQEAARIVDPAYHVNPACNYYFLVA
                     STFVLSAVGTWVTEKIVEPRLGVYQGEEKPEEIKPLTVEEKRGFRYAMITGLVLTAII
                     LIGLIPENGYLRDLKTGSVLKSPFMAHIVSFIFILGAALGIAYGWGTGKFKNDSDIMK
                     SMGKSMETLGGYMVLVFFAAQFVAYFNWTNLGLIIAVKLAGILKTSGLGSVPLIVSMV
                     FMTSFVNLFMGSASAKWAIMAPIFVPMFMILGYAPELTQAAYRVGDSCSNVISPMMSY
                     FALIMAFMQRYTKDAGLGSLIATMIPYTIAFLIFWTVALVIWLIFEIPLGPDARLFLQ
                     V"
     gene            complement(2882..4714)
                     /gene="glmS"
                     /locus_tag="PK754_05725"
     CDS             complement(2882..4714)
                     /gene="glmS"
                     /locus_tag="PK754_05725"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNB09035.1"
                     /translation="MCGIIGYIGRRQAMPIVMNGLKRMEYRGYDSAGVCFVENQSLQI
                     RKQAGKLYELEKDLVGVNFDATVGMGHTRWATHGVPNQTNAHPHFDEDNNFAVIHNGI
                     IENYASLKIELVKKGHTFKTETDTETVIHLVEEFYKSGMDFETAVREAIYCIKGAYGF
                     VFMCRHEPEKIIVARCGSPLVLGYGNEEFLVASDASAIVAHTRQVVYLEDNEMAILSR
                     QGIEVKTLDNKVIEKQVEEITFDISQIEKAGYEHFMLKEIFEQPNTIRDAMRGRLIAE
                     EGMPRLGGLVDHIDTLKKAKKIVFIACGTSWHAALTAEYMIEEFARINVEVEYASEFR
                     YRNPIVEEGTVVFVISQSGETADTLAAMREAKRRGAAVFGICNVVGSSIARETDAGVY
                     IHAGPEIGVASTKAFTSQLTVIALITVLLARLRNMSSAEGKQIVEEMDMLPEKVKEIL
                     KASDYIREIAELYKDRRNFLYLGRGYNFPVALEGALKLKEISYIHAEGYPAAEMKHGP
                     IALIDEEMPIVFIAPQDAIYDKVVSNIEEVRARGGKVIAITTEGNTDIRSKADHIIYI
                     PKTMDFLIPILSVIPLQLLSYHMAVLRGCNVDQPRNLAKSVTVE"
     gene            4969..7197
                     /locus_tag="PK754_05730"
     CDS             4969..7197
                     /locus_tag="PK754_05730"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNB09036.1"
                     /translation="MNLFQEQGATAPIVRDKAHYDHIFEEALNSYQSYIRIPDTETLR
                     KAYEYSYLSHLGQFRKSGLPYFDHIIEVVKILIEQNMDTVTLVAAFLHDVIEDTGKTY
                     DDIAKEFGNDVAGIVDGLTKIAGFEFGSLEIKQAENFRKMLLSMIKDIRVIIIKFADR
                     IHNMRTIDSLPRKKQERIATETRDVYAPLAHRFGMARTRWELEDLVLKVLDPESYWEL
                     SRLVSEKRTEREFYIQSVAKPLIEKLKDNGVEAQIIGRPKHFFSIYNKMKKRNKPFNE
                     IYDLLAIRIMVQEKTDCYRALGFVHSTYTPVTERFKDYVAVPKINGYQSIHTTVIGPD
                     GKMVEIQIRTHEMHQIAEEGIAAHWLYKEGKNQMDQIDKQIGWIRQLIERQRDQSDPG
                     EFLEDLKIDLFQDEVFVFTPKGDLIPLPKGSTPIDFAFAVHSEVGLHCIGAKVNGKIV
                     PLHTELRSGELIEILTSEHQSPSTHWLEIVKSSKARNHIKRYFRNIEIEQSIVLGKDL
                     LEQELRLVQLRKPFADYVDDLIKMATELTFENLDMMLSSIGRGNTSAQSIAQRLAQKN
                     IAPEKPQESSSFFKRFIKRSSSESKGIAISGVDNMMIHFAKCCNPIPGDQIMGYVTRG
                     RGVHLHRNDCHNILDAVKKEPERALAAKWQVDETKNFNVQIRVVGRDRKNFLVDVAQK
                     ISSTDTNIVSAEVRTTGNETVHLFVLQVRNIAHLNMILDKIRRVPNVITASRADTSGV
                     AN"
     gene            7218..7979
                     /locus_tag="PK754_05735"
     CDS             7218..7979
                     /locus_tag="PK754_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB09037.1"
                     /translation="MKIQFLGTGTSHGVPMIGCTCAVCASENPYNKRLRTSVIVEVKE
                     KKILIDTSIDLRQQALLHRIHHVDAVLFTHHHVDHIFGLDETRSFNRLQKKRLPCYAG
                     AYTIEQMKKVFSYIFEYPDIPGGIPMIDFIPVDKPFAIEDVQIIPITVMHGKLPIYAY
                     RIGDFIYATDCSGIPETSWEYFYNAEILVLDCLRYRTHPTHFNVEQAVEAARKIGAKK
                     TYFTHISHELDHDIVNATLPENIKLAYDGMIVESN"
     gene            7990..10350
                     /locus_tag="PK754_05740"
     CDS             7990..10350
                     /locus_tag="PK754_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB09038.1"
                     /translation="MRKNFFLVPFLLVLQSCAFFNTFYNARKSYKLGKESIERSSRTS
                     REAPSNTPEMKLGFFDSEPDNIPGDAKTAFDISIEKTNKVVVLHPTSGWVDDALLLLG
                     KSYYLRSGEGDYLEAKNRLEVLMTRFPASELISEAKLWYGKTLFKLQQFQEAQEILRP
                     IEALNTTEDIRAEANLILGDIALLDENLNVAKAYYAQAQKLAKDENLRKTTLYKSAYC
                     FYKDQKYSDCVVFLNKLNQNNLSLSERFEILVFRSRALKMAGQYKTAIDHLDKILGDP
                     KYKTYFARAEFEIADILRMQGRSSDAEKQFIFIVNTYPAAPVGDCYYMLGLLHDTTQT
                     RPSADFIPDHNRAMKFYTLVRNKYASSYYAPTAVARLTFFEKMNLLKDVMSVNRRLVE
                     IIDGRIKTLESGDTATFVPDEFLVQLVKVDTSVQTNLLEDKDKAETLSDKVKQDLKTI
                     NMVSTELTEKQNEINSDIFSLVELTHRDSLLNFRKKVNERLAEYHILLAEYYANELHE
                     YDSSLAYYTKVTELFKESTSEEIAYYGLARIHQIKGFPSYLSLYKEAYEKFPNGVMAK
                     TGETILGLKAANADRYDDVFEEAQMLMVSDTQWVKAADLFQTIARSDSTKHKWPSVYA
                     MGLLEEKKKNNPQKAFLYYNTLLYGAPSWPSAEKLRQKVNAYKKAWNIQNDSAGYTID
                     TTLIRWHHDISVTQIDQKADSVRTHQEIGKNSMPITTESARRDTNRLQMTDFELEEFE
                     NLLKKKSGADSTKVGKKLKPDESLKNKKQRRIKEDESDATPIIKEE"
     gene            10350..10775
                     /locus_tag="PK754_05745"
     CDS             10350..10775
                     /locus_tag="PK754_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNB09039.1"
                     /translation="MRYTVLLLIFMLFLYACVPNPQYGTVASRTRGEIPGKEIIKKGY
                     QTGVASYYAHKFHGRKTANGEIFDMHKLTAAHRTLPFNTLVKVTNLKNNKTIQVRIND
                     RGPFAKDRIIDLSLAAAQKIDMIGSGTAEVRLDIIKEGP"
     gene            10796..11506
                     /locus_tag="PK754_05750"
     CDS             10796..11506
                     /locus_tag="PK754_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09040.1"
                     /translation="MLGQAPFAVASLMLINFALTLIFFFRSVRYSEAGRSSSLRFTVF
                     FVVFLWVLLQAVLSYIGFYTQFSALPPRLILTGVGPAVIAVLVFLIIPSLRNIINGFR
                     LEDLILLSVVRIPVEIMLHQLFTVGLVPEDMTYTGLNWDIFSGITAPAMMWVAKKNFT
                     WSRSVLIVWHVLTLGLLINIVSIAILSAPFPFQQINFDQPNIAVFSFPFVFLPTFIVP
                     MVLWATLTGLVKIYNVKK"
     gene            11507..12205
                     /locus_tag="PK754_05755"
     CDS             11507..12205
                     /locus_tag="PK754_05755"
                     /inference="COORDINATES: protein motif:HMM:NF018626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HNB09041.1"
                     /translation="MMKFLSNAFIFLVFIVSCKTKSDNLLQTLIPTTTIEAKSDNKIA
                     QAAKKYVENPFSYDITMSYHVTTYKNGQNTKTAVYPMGDINPEIGVCTDVVIRSLRIA
                     GCDLQELVHRDASKHFSFYPYFIWGQGKPDPNIDHRRVPMLNAFFKRHAKTLTIATDD
                     KHINEWQAGDIIIWDLTGSGKMDHIGIVSDTRTKAGNIPLIIHNYPSPGYVTQENVLN
                     RWVIKAHYRYDGCN"
     gene            12291..12902
                     /locus_tag="PK754_05760"
     CDS             12291..12902
                     /locus_tag="PK754_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09042.1"
                     /translation="MPVQASANFSFPDTLLGRWSAVLPDTTYNPGPPMRVVQSTTNLI
                     ISSKLMYTQAQTISEGPIIFLDTVDGGKYKNRKLLDEELKSIGGKMDFLQNHFRFFTE
                     FNDTLFVIDKINHLRKSDHYWVLNRRDAHHKAWILGWMYLRNDTLGFMYPTRADSLKI
                     TKHVNSNNVRIRNDGLIELHVSDSVFKQLLDLDWNNRRFYVRQ"
BASE COUNT         3723 a         2703 c         2888 g         3824 t
ORIGIN      
        1 acgtcaaatc gttgagcgca gtgatgtgcg aattgattcg acgctttcgc ggctttcttc
       61 ctcaaccaaa attttcaaaa acatattgag ccgcacggat gcacagattg attcgacatt
      121 tggcaatgta cgactgatca cgcaatccgt tatggatgta actcaatcat tggacagtat
      181 tgtaagtaaa gtgcaaagcg gcgaaggcaa tctgggccgg atgatctatg atgatcagtt
      241 atattggcga atcaatcgca ccacacaaca attagattct ttggtcggct cactgcgcga
      301 aaaaggcgtg aaggtcaatg ttaaattgtt tggcgactaa gcgcagtaca agattatgaa
      361 ttttcttctt tccattatgt taagcttgtt tgccggtgta ttgatcattg ccggtggatg
      421 ggttttttca tccaaaaaaa attgggaatc ccgctatctt gattttttta tcgctttggg
      481 tgcgggatat attctcggtg tagcgcttct cgatatgata ccggaagctt ttcatgcgac
      541 gccccatgcg atgtattacg tggtcttagg ttatctgatc gttcacttat ttgaacacgt
      601 cttaacaccg cactttcatt ttggcgaaga aacacacaat caccttattt ccaacgtggt
      661 tagtacgtcg gcgctgatcg gacttatggt acataatttt tttagcggtg tggcgatcgg
      721 ttcgggcatg ttgaccagtg aaaaagtagg atggatgata tttgcaggca cggtgcttca
      781 taaacttccc gaaggtttta cgattgcttc gatcatgttt gcgtctcatc accgccgtac
      841 atacggcgtt gtaggtacga ttttgctggc gcttgcatcg ctgctgggaa ctgtaatgat
      901 ttatttattt gaaatggagt ctttgcccgt caaagatgtt gctttggcca tgtcggcggg
      961 aacttttatc cacgtagcca ccacagatct tattccgcgt atcaatgatt ccggttctcg
     1021 tcgtatgcca ttagtagttt ttctaggtgt cggattattt ttgataagtt atatgttgat
     1081 gcatccgtaa gcatcttgct tggttatcgg ttctattcct ttcggattct cgaaaattaa
     1141 ttatcatatt ggatatttcg tcgtatcatt tcggagcttt ggtatgttaa aaaaaatatt
     1201 tagccgggat acattacaaa aggttctcaa cttcattgag cgagtgggta atctattacc
     1261 tcatccggct acattgtttg gtctttttgc attgaccatc gtgatcgtat cggggatttt
     1321 cagcgcaatg gatatatccg tacaacaccc gggcaccggt gaggttattc gtccggtcag
     1381 cctgttaaca gttgaaggaa tgcatcgcat tttatctaac cttgtaacca attttacggg
     1441 attcgtccct ttgggtaccg ttcttgttgc gatgctgggt attggtattg ccgaaggcag
     1501 cggtttgatc ggctctgtat tgcgtctctt agtcatttca gcgccaaaac gattattgac
     1561 tttcgtgatt gtttttgccg gcgtaatgtc caatgtggcg ggcgaagtcg gttatgttct
     1621 tttgatacca ttagccgcag taatattttt ggctgtaggt cgccacccca ttgcgggttt
     1681 agcggcagcg tttgccggcg tgtcgggcgg ttacagtgcg aatttattat taggcaccgt
     1741 ggatcctctt ctggcggggc ttactcagga agcggcgcgt atcgtggatc cggcttatca
     1801 tgtaaatcct gcttgtaact actatttttt ggttgcttcg acttttgttt tgagcgctgt
     1861 aggtacatgg gttaccgaaa aaatagtaga accgcgtttg ggtgtttatc aaggtgaaga
     1921 aaaacctgaa gaaatcaaac cgctcacagt agaagaaaaa cgcggttttc gttatgcaat
     1981 gataaccggt ttagtactaa cagcgattat cctcataggc ttaattcctg aaaacggtta
     2041 tctgcgcgat ctgaaaaccg gttctgtttt gaaatcgccg ttcatggcac atatcgtttc
     2101 atttattttt atactcggtg cagcgctggg aatcgcttat ggttggggta ccggaaagtt
     2161 taaaaacgac agtgatatta tgaagagcat gggcaaatcc atggaaacac taggcggcta
     2221 tatggtgctt gttttttttg cagcgcaatt tgtcgcatac ttcaactgga caaatcttgg
     2281 tctcatcatc gctgtaaaat tagccggtat tctgaaaacc tccggattag gaagcgttcc
     2341 cttgattgtt tcgatggttt ttatgacatc atttgtaaat ttatttatgg gcagcgcatc
     2401 agccaaatgg gcgatcatgg cgccgatatt tgttccgatg tttatgatac ttggttatgc
     2461 gccggagttg acccaagcgg cttatcgtgt aggcgacagt tgctccaatg tcatttcgcc
     2521 gatgatgtct tattttgcgc tcatcatggc gtttatgcaa cgctatacca aagacgcagg
     2581 tttgggttca ctcatcgcca cgatgattcc ttatacgatt gcatttctta ttttttggac
     2641 cgtagcgctg gtaatctggc ttatatttga aatccccctg gggccggatg cgcgattatt
     2701 tttgcaagtg taataccatc agattttaaa taaaaaacct gagttgatga aactcaggtt
     2761 tttttatact ctctcaaatt tttactttta gttgtgctaa aataatattt gtcttcaggc
     2821 ggttattttt tgatcaaact tttgccggta ccgtttgatc tgttccattt gcaattttga
     2881 tttattctac cgtcacgctc ttagcgaggt tacgcggttg atccacattg catccgcgaa
     2941 gaacagccat atgataagac aaaagctgta acggaatcac ggacaaaatc ggaatcagaa
     3001 aatccatcgt tttgggaata taaatgatat ggtccgcttt gctgcggata tcggtattgc
     3061 cttccgttgt aatagcaatc actttgccgc cgcgtgcacg cacttcttcg atattactga
     3121 caaccttatc atagatggca tcttgtggcg cgataaaaac gatcggcatt tcttcgtcaa
     3181 tgagtgcgat cggcccgtgt ttcatttctg ctgccggata accttcggca tgaatgtagg
     3241 aaatttcctt tagtttgaga gcgccttcca aagccaccgg aaaattatat ccgcggccaa
     3301 ggtacaggaa attcctacga tccttataca attcggcgat ttcacggata taatcgctgg
     3361 ctttgagaat ttcttttact ttttccggca acatatccat ttcttcgacg atttgtttac
     3421 cttcggcgga ggacatattt cgcaaccgag ccagaagtac ggtgatcagc gcgatgacgg
     3481 taagttggga agtaaatgct ttggtcgaag cgacgccaat ctcgggtccg gcatgaatat
     3541 aaacgccggc atcggtttcg cgtgcgatcg aacttccgac cacattacaa ataccgaata
     3601 cggcagcccc tcgccgtttg gcttcgcgca tagcggcgag tgtatcggcg gtttcgcctg
     3661 attggctgat cacaaaaaca acggtacctt cttcgacgat gggattgcga tatctaaact
     3721 ccgaagcgta ttctacttcg acattgattc gcgcaaattc ctcgatcata tattcagccg
     3781 tcaatgccgc atgccaactg gtgccgcatg cgatgaatac gatttttttt gcttttttga
     3841 gtgtgtcaat atgatcaaca agaccgccaa gtcgcggcat gccttcctcg gcgatcaggc
     3901 gaccacgcat cgcatcacgg atcgtgtttg gctgttcgaa aatttcttta agcataaaat
     3961 gctcatagcc ggctttttcg atctgactga tatcaaaagt gatttcttcg acttgttttt
     4021 cgataacctt attgtctaaa gttttgactt cgataccttg acgtgacaat atggccattt
     4081 cattgtcttc cagatatacg acttgtcgtg tgtgtgcgac gatcgcggag gcatcagaag
     4141 ccacgagaaa ttcttcgttg ccataaccca aaaccagcgg gctaccacag cgggctacga
     4201 tgattttttc aggttcgtgg cggcacataa aaacgaaacc atacgcacct ttgatgcagt
     4261 atatggcttc tcgtacagcg gtttcgaaat ccataccgga tttgtaaaat tcctcgacga
     4321 gatggattac tgtttcagta tcagtttcgg ttttaaatgt atgacctttt ttcacaagtt
     4381 cgattttcag agacgcatag ttttcgatga taccgttatg tatgacagcg aaattgttgt
     4441 cttcatcaaa atgaggatgg gcgttcgtct gatttggcac gccgtgcgtg gcccagcgtg
     4501 tgtgccccat gccgacggtt gcatcaaaat taacaccgac caaatctttt tcgagttcgt
     4561 aaagtttgcc cgcttgttta cgtatttgta atgattgatt ttcgacaaaa caaacgccgg
     4621 ccgaatcata accgcgatat tccatccgtt tgaggccatt catcacgata ggcatggctt
     4681 gacgtcttcc gatataacct ataatcccgc acataaatcc tccaaaattt gagccccatg
     4741 atagtaaaat aggctcgttt tagcaaggtt tttgggtttg aataataact cgtttttttc
     4801 gttgtaacaa tacgatcaag tcaacaaaat tcttgtcaaa actctttgta taacatatat
     4861 ttataaatca gtttgtgtta tgggttaaaa cctaagagcc attcttgaag ctaagccaaa
     4921 attaatcata tgatcgaaac ccaaacaaaa aagaacggaa acgctgcatt gaacttgttt
     4981 caagaacaag gcgcgactgc acctattgta cgtgataagg cgcattacga tcatattttt
     5041 gaagaagctc tgaattctta ccaatcctat attcgcatac cggataccga aacactgcgt
     5101 aaagcttacg aatacagtta tctttcacac ttggggcaat tccgtaaatc cggtttaccg
     5161 tattttgatc acatcatcga agtggttaaa attttgatcg aacaaaacat ggatacggtg
     5221 acgctggtcg cggctttttt gcatgatgtc atagaagata ccggaaaaac ctacgatgat
     5281 attgctaaag agttcggcaa tgatgtcgcc ggcattgttg acggattgac caaaatcgca
     5341 ggttttgaat tcggcagttt ggaaatcaaa caagccgaaa atttccgcaa aatgcttctt
     5401 tcgatgatca aagatatacg tgtgatcatc atcaagtttg ctgatcgtat tcacaatatg
     5461 cgcacgatcg actccctgcc gcgcaaaaaa caagaacgca ttgcgacgga aacgcgcgac
     5521 gtgtatgccc cgctcgccca tcgttttggt atggctcgta cgcgatggga attggaagat
     5581 ctcgtgctca aagtgctgga tccggagtcg tattgggagt tatcacgctt agtcagtgaa
     5641 aaaagaacgg agcgcgaatt ttatattcag tccgtggcca aaccgcttat cgaaaagctc
     5701 aaagataacg gagtcgaagc gcaaatcatc ggccggccca aacacttttt cagtatttat
     5761 aataaaatga aaaagcgaaa caagccgttt aacgaaattt acgacttgct tgctattcgt
     5821 attatggttc aggaaaaaac ggattgttat cgcgctttgg gttttgttca cagtacatac
     5881 acgccggtta ctgaacgatt caaagattat gttgccgttc ctaaaatcaa cggctaccaa
     5941 tctatccaca caaccgtgat cgggcccgac ggtaagatgg tggagataca aattcgcaca
     6001 cacgagatgc atcagatagc cgaagaaggt atagctgcgc actggctgta caaagaaggc
     6061 aaaaatcaga tggatcagat tgataaacag atcggatgga ttcggcagtt aatcgaacgc
     6121 cagcgtgatc agtcggatcc cggtgaattt ctcgaagatc tcaaaattga tttatttcag
     6181 gacgaagttt ttgtttttac gcccaaaggg gatcttattc ctttacccaa aggctctacg
     6241 ccgatagatt ttgcttttgc cgtgcactcg gaggtaggtt tacattgtat cggagccaaa
     6301 gtcaatggca agatcgtacc tttgcacacg gaactgcgca gtggtgaact tattgaaatt
     6361 ctcacctctg aacatcaatc gccaagcacg cattggctgg aaatagtcaa atcatccaaa
     6421 gcgcgcaacc acatcaaacg ttattttcgt aacatcgaga tcgaacaaag tatcgttttg
     6481 ggtaaagatc ttctcgagca ggaattgcgt ttggtacaac tgcgcaaacc gtttgcggac
     6541 tacgtggatg atttaatcaa aatggctacg gagctaacat ttgaaaatct ggatatgatg
     6601 ctttcatcca tagggcgtgg caatacctca gcccaaagta tcgctcaacg cttagcgcaa
     6661 aaaaatatag ctccggaaaa acctcaggaa agcagttcgt tttttaaacg ttttatcaaa
     6721 cgcagttctt cggaatccaa aggcattgcc atcagcggtg ttgacaatat gatgattcat
     6781 tttgccaaat gctgtaatcc gataccaggc gatcagatta tgggctatgt cacgcgcggg
     6841 cgcggggtac acttgcaccg caatgactgt cacaatatac tggatgccgt caaaaaagaa
     6901 cccgaacgcg cgttagcggc caaatggcaa gtagatgaaa ccaaaaactt caacgttcaa
     6961 atacgtgtcg taggacgcga tcgtaaaaac tttcttgtgg acgtggcgca aaaaatatcc
     7021 tccacggaca ctaatatcgt cagcgcggaa gtgcgcacga cgggaaacga aaccgtacat
     7081 ctttttgtat tacaagttcg gaatatcgcg catctgaata tgatactcga taaaatacgg
     7141 cgcgtgccca atgtcatcac cgcatcccga gcggatacga gcggcgtagc caactgaata
     7201 acggattgtc aaaaggaatg aaaatacaat ttttaggaac gggcacttcg catggcgtgc
     7261 cgatgatcgg ttgtacctgt gcggtatgtg catcggaaaa tccgtataat aaaagattgc
     7321 gcacgtctgt aatcgtcgaa gtaaaagaga aaaaaatatt aatagatact tcgatagatt
     7381 tacgacaaca agcgctttta caccgtattc atcatgtaga tgccgtactt tttacgcatc
     7441 atcatgtgga tcatattttc gggcttgatg agacacgttc atttaaccga ttacaaaaga
     7501 aaaggcttcc gtgttatgcc ggagcataca cgatcgagca gatgaaaaaa gttttttcct
     7561 acatttttga atatcctgat attccgggcg gaataccgat gattgatttt ataccggtag
     7621 ataaaccttt cgccatcgaa gacgtccaaa tcatacccat cacggtgatg cacggaaagt
     7681 tacctattta cgcctatcgt atcggtgatt ttatatatgc aaccgattgt tcgggaatcc
     7741 cggaaacatc atgggagtat ttttacaacg ctgaaatttt ggttctggat tgtttgcgct
     7801 acaggacgca ccccactcat tttaatgttg agcaagcggt cgaagcggca cgaaagatcg
     7861 gcgccaaaaa aacatatttt acacatattt cacatgaatt ggatcatgat atcgtgaatg
     7921 ccacgttacc ggaaaacatc aaattagctt atgatggtat gattgttgag agtaattaga
     7981 aagagaagca tgcgcaaaaa tttttttctt gttccctttt tattggttct gcaaagctgt
     8041 gcgtttttta atacatttta taacgcccgg aaaagttata aactaggcaa agaaagcatt
     8101 gaacgctctt cgcgcacatc acgggaagcg ccctccaata ccccggaaat gaaattaggt
     8161 ttttttgatt cggaaccgga taacataccg ggcgatgcca aaaccgcatt tgatatctct
     8221 atagaaaaaa ccaataaagt cgtcgtgctt catccgacaa gcggatgggt ggatgacgca
     8281 ctcttactgc tcggcaaatc ctattattta cgttccggtg aaggggatta tcttgaagct
     8341 aaaaaccgcc tagaagtttt gatgacgcgg tttcccgcgt ccgaactgat atcggaagca
     8401 aagctttggt acggtaaaac attattcaaa cttcagcagt ttcaggaagc tcaggaaatt
     8461 cttcgtccga tcgaagcgct caatacaacg gaagacatac gtgctgaagc caacctgata
     8521 ctgggcgata ttgcacttct tgatgagaac ctcaacgtag cgaaagccta ttatgcacag
     8581 gctcagaagc tcgccaaaga cgagaatcta cgcaaaacga cgctgtataa atcagcctac
     8641 tgtttttaca aagatcaaaa gtattcagat tgcgtagtat ttctaaataa actcaatcaa
     8701 aacaatctat ctctttccga acgctttgaa atacttgttt tccgatcacg ggcgctaaag
     8761 atggctggac agtataaaac agccattgat cacttggaca aaatcttggg tgatccaaaa
     8821 tacaaaacat actttgcaag agctgagttt gaaattgctg atattttacg tatgcagggt
     8881 cgttcaagcg atgccgaaaa acagtttatt ttcatagtca atacttatcc cgcggctccg
     8941 gtcggggact gttattacat gttggggttg ttgcacgata cgacgcaaac aagaccatcg
     9001 gccgatttta ttcccgatca taatcgcgca atgaaatttt atacattggt acgtaacaaa
     9061 tatgcttcgt cgtactatgc accaacagct gttgcccggt tgacgttttt tgagaaaatg
     9121 aacttgctca aagacgttat gtcggtcaat cgtcgtttag tagagattat tgacggccgg
     9181 ataaaaacct tggaatcagg cgatacagct acttttgttc cggatgaatt tttagtgcaa
     9241 ttggtcaaag tagatacaag tgtgcaaact aacttgcttg aggacaaaga caaagccgag
     9301 acgctaagcg ataaagttaa gcaagatctg aaaacgatca acatggtatc aacggaatta
     9361 acggaaaaac aaaatgaaat aaattcagat attttttcgc tcgttgagtt aacacacagg
     9421 gatagtttgt taaattttag aaaaaaagtt aatgaacggt tggccgagta ccatatttta
     9481 ttagccgaat actatgcaaa tgaactccat gaatatgatt cttcattggc ttattatacg
     9541 aaggtgaccg aattatttaa agagtcaacc agtgaagaga ttgcttatta tggtttggcg
     9601 cgaatccatc aaatcaaggg atttccaagt tatctgtctt tgtacaaaga ggcttacgaa
     9661 aaatttccca atggcgtcat ggctaaaacg ggggaaacca ttctcggatt gaaggccgca
     9721 aatgcagatc gttatgatga tgtttttgag gaagcgcaaa tgctgatggt atccgacacg
     9781 caatgggtga aagccgctga tttgtttcaa accattgcac gaagcgattc gaccaaacat
     9841 aagtggccct cggtttatgc gatgggttta ttggaagaaa agaaaaaaaa taatccgcaa
     9901 aaagcttttc tgtactataa cacgttattg tacggtgcac catcatggcc gtctgccgaa
     9961 aagctgcgac agaaagtgaa cgcctacaaa aaagcgtgga atattcaaaa cgattcggcc
    10021 ggttatacga tcgacacgac attgatccgg tggcatcatg acataagcgt gacgcaaatt
    10081 gatcaaaaag cagattccgt cagaacacac caagaaattg gtaaaaacag tatgccgatc
    10141 acgaccgaat ctgcgcgtag agataccaac cgtttacaaa tgaccgactt cgaactcgaa
    10201 gaatttgaaa accttttaaa aaagaaatcc ggagcggatt ctacgaaagt tgggaaaaaa
    10261 cttaaacccg atgagtcgct aaagaacaaa aaacaacgtc ggatcaaaga agacgagtcg
    10321 gatgcgacac cgatcatcaa agaggaatga tgcgttatac tgttttgctg ttaattttta
    10381 tgctgttcct atacgcgtgt gtacccaatc cacagtatgg tacggttgcg tcgcgcacac
    10441 ggggtgaaat tccggggaaa gaaataataa aaaaaggata tcaaaccggc gtcgcgtcat
    10501 attatgcaca taaatttcac ggcagaaaga cagcgaatgg tgaaattttt gatatgcata
    10561 aattaactgc ggcacatcgt acgctaccgt ttaatacgtt agtcaaagta accaatctaa
    10621 aaaacaacaa aacgatacaa gttcgtatca atgaccgcgg cccttttgcc aaagatcgaa
    10681 ttattgatct atcgctggcg gccgcgcaaa aaatagacat gatcggtagc gggacagctg
    10741 aagtaaggct cgatattatc aaagaaggtc cgtaaatcca tcggaggaaa tcatcatgtt
    10801 aggacaagcg ccgtttgccg tcgcatcgct tatgttgatc aattttgcgt tgaccttgat
    10861 tttctttttt cgatctgtgc gttactcgga agccgggcga tcttcttcac tgcgcttcac
    10921 ggttttcttt gtagtttttt tgtgggtgtt gttgcaagct gttttgagtt acatcggctt
    10981 ttatacccaa ttttcggcgt tacctccgcg actgatactt accggggttg gccctgcggt
    11041 tattgcggtt ttagtttttc ttataatacc gtcattgcga aatataatta acggatttcg
    11101 attggaagat ttgattttgt taagcgtggt acgcataccg gttgaaatca tgttacacca
    11161 gttgtttaca gtcggattgg ttccggaaga tatgacatac accggattaa actgggatat
    11221 cttttccggt attacagcgc cggcgatgat gtgggtcgca aaaaaaaact tcacttggtc
    11281 gcgtagtgtt ttgatcgttt ggcatgtact cactttggga ttactgataa atatcgtgtc
    11341 catagcgatt ttatctgcgc cctttccgtt tcagcaaatc aattttgatc agcccaatat
    11401 cgccgtgttt tcatttcctt ttgtttttct tccgacgttt attgttccga tggtattatg
    11461 ggctacgttg acggggttag tgaagatata taatgtgaaa aaatagatga tgaaattttt
    11521 aagcaatgcc ttcatttttt tagtttttat agtttcctgt aaaacgaaat cagacaattt
    11581 gttgcagaca ttaataccca cgacaactat agaagcaaaa tcagataaca agattgcaca
    11641 agctgcaaaa aagtatgtag aaaacccttt ttcatatgac ataacaatga gttaccacgt
    11701 tacgacatac aaaaacggtc aaaacacaaa gaccgccgtt tatcctatgg gagacatcaa
    11761 tccggaaatc ggtgtatgta cagatgttgt gatacgaagc ctgagaattg ctggttgtga
    11821 tttgcaggaa ctcgttcatc gtgatgcatc taagcatttt agcttctacc cttattttat
    11881 atggggacaa ggaaaacccg atcctaacat agatcatcgt cgcgtcccaa tgcttaatgc
    11941 tttttttaaa agacatgcta aaacgctaac gatcgcaacc gatgataaac acataaatga
    12001 atggcaggcc ggagatatca tcatctggga tttaacgggt tccggtaaaa tggatcatat
    12061 tggtattgtt tctgacacgc gcacgaaagc aggtaacata ccattaatta ttcataacta
    12121 tccttcccca ggttatgtta cacaggagaa cgtgttgaat cgctgggtga ttaaggcgca
    12181 ttatcgatac gatgggtgta attaaaatgc gactaacaac actttatagg ttactatttt
    12241 taatgttttc gctttcctgc cagcatcccg gggttttatt tgaccaaccg atgccggttc
    12301 aagcgagtgc gaatttcagt tttccggata cattacttgg gcggtggtct gctgttttgc
    12361 cggataccac atacaatccc ggtcctccaa tgcgtgttgt acagtcaaca acaaatctga
    12421 tcatttcatc aaaactcatg tacacgcaag cgcaaacaat ttccgaaggt ccgattattt
    12481 ttttagatac cgtagatgga gggaaatata agaatagaaa attattggat gaggaattga
    12541 aaagcatcgg cgggaaaatg gattttttac aaaaccactt tcgttttttt acagagttta
    12601 acgatacatt atttgttata gataagatca atcatttgcg caaatccgat cattattggg
    12661 tactcaatcg tagggatgcc catcataaag catggatact gggttggatg taccttcgca
    12721 atgatacttt aggttttatg tatccgacgc gtgccgatag cttaaaaatc accaaacatg
    12781 tgaattcaaa caatgttcgt attcgcaatg acgggttaat agagctgcac gtatccgata
    12841 gcgtattcaa acaacttctt gatttggatt ggaataatcg aagattctat gtacggcagt
    12901 aggataaatt ccttcttcat aatcccattg tagcttaacg aatatccgct atatttcttc
    12961 atatgataaa attattttat aacggaaaaa tatacaccgg cgaaaagagt caacctttct
    13021 gtgaagccat tgttgtcagc ggcggaacga tcgtcgcatc cggtacgaac gaagagatgc
    13081 gttcggcttt tccttttgca cagcgcattt cattggacgg aaagccggtt ttacctgc
//
LOCUS       DAOQNQ010000061        12823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1724_length_12823_cov_5.612547, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000061 DAOQNQ010000000
VERSION     DAOQNQ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12823
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1724_length_12823_cov_5.612547"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1640
                     /locus_tag="PK754_05765"
     CDS             <1..1640
                     /locus_tag="PK754_05765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB09043.1"
                     /translation="ATAAGLDGLKGHRSVGGLRASIYNAFPKAGVETLVEFMKEFERK
                     IREQSFILNLVSDAVFVLDYNGRVMLWNPSAAQLYGWCETEIIDQMCLDYVFRNQTHD
                     YHAIMHSVLKKGFWKGDIGLYNKAGKKIVVESRWNLLVGLDTHGSSILVFSSDVTELR
                     QSERTLLQNQRVETIGKLAGGIAHDLNNILTTIRIGIDTLHQERASVHDASVMEVVSK
                     SIARGSDLVKKILSYSKGQSSDKKPVQLIDVVLEAVKIIQETFPKSIDFGLDMQFDLH
                     PIMADSTQMHQLIMNLCINARDAMPQGGRLFISIRKEDCPGQRYVSGFKHFVVISISD
                     DGVGISQENLNRIFDPFFSTKEPDKGTGLGLSTVQSIAKNHGGFVKVESTVGKGTVFK
                     VYLPYSDVIAEANIKNTKFPRNTSDGEGKRILVVEDEESIRNMLKEALEEKDYLVETS
                     QHGLDAVDKVQFNGEDYFSLIIMDMNMPIMNGVLAIEEIRKRNMKVKIIASSGEMDMW
                     MKSYLNSNLIDRTIEKPFSLEKLFSVIEDVLLPVEMA"
     gene            1724..1867
                     /locus_tag="PK754_05770"
                     /pseudo
     CDS             1724..1867
                     /locus_tag="PK754_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014460836.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
     gene            2293..2874
                     /locus_tag="PK754_05775"
     CDS             2293..2874
                     /locus_tag="PK754_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09044.1"
                     /translation="MKMITWGILLCLMTACGSGGTGNEDLSKEAQQAFAEGNYEDALN
                     IFLSLAESEPVTGYTGAGWSAVRLHDYANANVYFQTIAASNDADAYAGWSLTGWALSD
                     FNSAIQRADTVLSKDADFVLKLDDGVAAADLILVQALCYYETNNFSMTLQKIKLIDSG
                     FNADLNGSDIAQVLAAKLEQLSVLFSKTLAKGQ"
     gene            2877..8492
                     /locus_tag="PK754_05780"
     CDS             2877..8492
                     /locus_tag="PK754_05780"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HNB09045.1"
                     /translation="MNFFTIHKHNTIRIIISSVVILFFASTLHAQSRVSFLTGEFEKS
                     GRETNEAFLRRILRASVEISFKRLSRETDRYGKTHDGFQMMYRSVPVEDAIIKVHSDH
                     RGIYAVTGETALPADIAITPKIEEAQALSKATGHIGAQAYKWQDADEEKILKEVTDNP
                     QASFYPKAETVVVNIAKEGESPDFRLAYKFDIYASKPVSRDWVYVDASSGEVVRKINR
                     IYHGTATGTADTRYAGTRTIKTDSIAPNQFRLKDLTRGNGVITYNLNHSVNDADSTHF
                     VDNDNQWTAAEYHNGNSDDGALDAHWGAEVVYDYWKNVHNQSSWDGNNAPMINFVHYN
                     NNNPNAFWDGYSVHYGQFQSVNPWTELDVVGHEFGHGIMMGIASLNYSGEGTSLHEGC
                     ADIWGALVDNYGNQKFGLNRDPWVFSEKIAFSRSFNYLMTYKSIDWDFAHNSPHGNGQ
                     VARYWFYLLAMGGSGVNKFGSSFAVNPIGIAEAEKIFYQAFKHYVTPNSIFTDFRKAT
                     ELAAQDINPAHVAVVQSAWDAVAVPGTTGSVIGCTPSGTQQNNIQVDYVTIANKGEPF
                     YNAGWSSGGYIKKDYMFNLILGKKVKMVISYPDASTKHYAMWMDTDRDGQFEEPSERL
                     AGGIISATSSTDSIQLPQTLGGTFSYGAVPIRIAVSANPINGACDNVVGEVEDYSAYL
                     NPPQVLDGYHLNIGANSGAELIGKSKLGSLETSVLFYPSDGFKNLAGSIFLNDPGYTM
                     WGFAGESYPIILRSHKFVPSAHTEYYSVWIDYDTNKVFDSNELVFQGSGQDSIKGYLK
                     IPQNMTNRVTRMRILMSRNASITDPYTAPDFGEVADFNVKLFANPLTSDGYKAPRGNC
                     WHTYSTDPTYMMSDSITIGSLILKTGRSKDGGYHDLKGTSVLFVPLNTTQAAVRLTPF
                     IDPNLQPVDSVYYNIWVDYNGDKDFNDVGEHAFFHYGKSKADGVLTIPAGLAAGLSTR
                     MRLSVSYFTDHVHADSVVNYGQVIDLGFGIQAPFTRSTPLADVNVNEDFGTTTAAILK
                     NDLIGNTTGLSFTAAALSGGVTVNRSVDTVRIVSVADFNGQVKIAMTTSDGSYTLYDT
                     ITVTVIPVNDAPYVSADVRDTMLNEDAAKTFLMRMSDHFSDVDQDEFAVQSNVLSGSA
                     VVTISNDSLYFKPTANFNGNVIVGLMASDGSLSVSDTFAVQVLPVQDAPYRVVTLRDT
                     SFNEDVSNQLVTKLTPYFSDPDNDLLTYNVLVLSGVVSARVGQDSLYITAQADATGPS
                     SLRISATDGNTSVYDTITVTVIPVNDAPYVSADVRDTMLNEDAAKTFLMRMSDHFSDV
                     DQDEFAVQSNVLSGSAVVTISNDSLYFKPTANFNGNVIVGLMASDGSLSVSDTFAVQV
                     LPVQDAPYRVVTLRDTSFNEDVSNQLVTKLTPYFSDPDNDLLTYNVLVLSGVVSARVG
                     QDSLYITAQADATGPSSLRISATDGNTSVYDTITVTVIPVNDAPKLVVSLRDTTLTQN
                     FTRVLVEKLTDNFTDVDNGTLQYSVTSLTSGLTASLGNDSIYVTGSNLAIGTAKITVQ
                     ASDGSLTARDTLQITIIDVTAPSGFVAALATPLLPRVRFTIGGNEILSSASLTVNNTP
                     VTLTKRDSKRYSGQYDLTTLPTSLVVVGTVTDLGNNVSPAINRTIQVSTLSKPLAING
                     FELARASGVKESIVLVSEEEIGMNKSDVTIQAVTISFDETMTIRLSAPVKHRDKGRYR
                     IVRWADEEKYPDYSLISTWQECTIQYQTGVVITTIEKSGTYVLIYDSDAEVLPTEFVL
                     NQNYPNPFNPTTTIRYDIAVSGQVSLIVYNLLGQKVRTLVNGFATQGTYRLQWDGNND
                     MGQRVSSGVYLYRLESGGKRFTKRMMFLK"
     gene            complement(8788..9234)
                     /locus_tag="PK754_05785"
     CDS             complement(8788..9234)
                     /locus_tag="PK754_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09046.1"
                     /translation="MVLSSALLTSEDKVLRILSSYARFAEKKNVPSLFFNRLTVLDER
                     FLHLTPFCSDQQQKHFDHLMKLLIKNATNAQGAWHELYAVIEKGHMLGLHVVNFRNVG
                     SALLSAIKESSDNHCSDAIHDAWMDLITQILKRVAMMETDLGISVN"
     gene            9489..11324
                     /locus_tag="PK754_05790"
     CDS             9489..11324
                     /locus_tag="PK754_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNB09047.1"
                     /translation="MAAESVLNLDEVTIRLAGDSGDGMQLVGTELTNASALLGNDVGT
                     LPDYPAEIRAPAGTLPGVSGFQLHIGSFDIHTPGDEVDVLVAMNPAALKANIRSLKPN
                     GVLIVNAESFGERDLKLANCATNPLEDGTVDGYQLFRVDMSSHTKAALKDTGLDHKSM
                     ERCKNFFSLGMIAWLYNRPMDGTIKFLKEKFAKKPLLAESNILAMKAGYAYCEATEAF
                     ANSYEIPPAKLPVGKYRNISGNEAIALGFVAASQKSGLELFLGSYPITPASDILHGLA
                     KYKNFGVKTFQAEDEIAAICAAIGASFAGELGITSTSGPGVCLKSEAIGLAVMTELPL
                     VVINVQRAGPSTGLPTKTEQSDLLQAMFGRNGESPVPIIAASRPADCFECAYEAARIA
                     VKYMTPVFLLADGYIGNGAEPWLIPDSDKLNEFSAHRTFTGEKFTPYARDEKTLARPW
                     IVPGTPGFEHRIGGLEKKNIAGTVNYEPENHDYMVRIRGEKVKRIAQEWNPLSIDGPD
                     SGDLLVIGWGGTYGAIRTAVDRSREKGLSVSRIHLRWINPFPQDLDAILKRFKHVLIP
                     EINLGQLRMLIRAEYLVDAIGLNKVTGQPFSVREIENKITEILKK"
     gene            11392..12417
                     /locus_tag="PK754_05795"
     CDS             11392..12417
                     /locus_tag="PK754_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNB09048.1"
                     /translation="MVQDIKPVYAKKDFESDQEIRWCPGCGDYSILAQVQRTLPEFQT
                     QKEKYVFVSGIGCSSRFPYYMNTYGFHSIHGRAPAIATGIKAANPELTVFVISGDGDS
                     LSIGGNHFLHVLRRNLDINYLLFNNRIYGLTKGQYSPTSETGKITKSTPMGSLEHPLN
                     PLSVALGAGATFVARAMDRDPKGLQDVIRAAIKHRGTSFVEVYQNCNIFNDGAFEHLT
                     EKDTKPDNTIWLEHGKPLVFGAKKNKGIRLDGFKPVVVNLEDGSSSVNDLLVHDMHNT
                     DPVMAFIYSQMTEAPGLPTPIGVFRSIQKSCYEEDMRTQIALAKDKMGPGDLKKLLHK
                     GDTWTVE"
     gene            12461..>12823
                     /locus_tag="PK754_05800"
     CDS             12461..>12823
                     /locus_tag="PK754_05800"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB09049.1"
                     /translation="MKKGKISITLAGLCFLSFTGCGKSEWVTFSDTKKDQITSIVESG
                     TSIWYGTRTGLVQFDKATEKRKVYTVNNGLPDNDIKYVTRDAKGKIWIGTNLGLSSFD
                     GQNWDVYSTAESKLPGDLI"
BASE COUNT         3559 a         2718 c         3085 g         3461 t
ORIGIN      
        1 aagctacagc agcaggttta gacggtctta aaggacatcg ttcagtaggt ggcttacgtg
       61 catctatcta caatgcattt cctaaagctg gagtagaaac tttagtagaa tttatgaaag
      121 aatttgagcg taaaattcgt gaacaatcat ttattttaaa tcttgtttcc gatgccgtat
      181 ttgtattgga ttataatggg cgtgtaatgt tatggaatcc atcggccgct caattatatg
      241 gatggtgtga aacggaaatc attgatcaga tgtgtttgga ttatgtgttt agaaaccaaa
      301 cccatgatta ccatgccatc atgcacagtg ttctgaaaaa aggtttttgg aagggtgata
      361 ttgggcttta taataaagcc ggaaaaaaaa tcgtcgtgga atcccgctgg aatctattag
      421 ttggcctaga cacccacggc agttcaattc tagtatttag ttcagatgtt acagagcttc
      481 gccaatccga acgtaccctg ttgcaaaacc aaagagtgga aacgataggt aaactggcag
      541 gaggtatcgc ccatgacctt aacaatattc tgacgacgat tcgtataggg atcgatacct
      601 tgcatcagga acgcgcatcc gtgcacgatg cttctgttat ggaagttgtg agcaagagta
      661 tagcgcgcgg gtcagatctc gttaaaaaaa ttctcagtta ttccaaaggc caaagcagcg
      721 acaaaaagcc ggttcaacta attgatgttg ttttagaagc agtaaagata atacaagaaa
      781 cttttcctaa gtctatcgat tttggcttgg atatgcagtt tgatctgcac cctatcatgg
      841 cagactccac acaaatgcat caattgatta tgaacctttg tatcaatgct cgtgatgcca
      901 tgccgcaggg gggaaggctt tttatttcta tacgcaaaga agattgcccg gggcagcgat
      961 acgtatctgg ctttaagcac tttgtcgtaa taagtatcag cgatgacggg gttggcattt
     1021 cgcaagaaaa tcttaatcga atttttgatc cgtttttttc tactaaggag cctgataaag
     1081 gtaccggact tggcctttca acggttcagt ccatagctaa aaaccatggt ggattcgtaa
     1141 aagtagaaag taccgtaggt aaaggaacgg tatttaaagt gtatcttccg tacagcgatg
     1201 tgattgccga agccaacata aaaaacacca agttcccgag aaatacgtcg gatggagaag
     1261 gtaaacgtat tcttgttgtt gaagacgagg aatcaattcg taatatgtta aaagaggcgc
     1321 tcgaagaaaa agattactta gttgaaacca gccagcatgg attggatgca gttgataagg
     1381 ttcagtttaa tggcgaagat tattttagtt tgatcattat ggatatgaat atgcccatca
     1441 tgaacggtgt cctggcaatc gaagaaatcc gcaaaagaaa tatgaaagta aagatcatag
     1501 catcgagtgg tgaaatggat atgtggatga aaagttatct taacagtaat ctcattgacc
     1561 gtactattga aaaaccattt tcgcttgaaa aattatttag cgtgatcgaa gacgttctat
     1621 tacctgtcga aatggcgtga agctttatgg tcatgcttcg attgggattg cgtacgaaca
     1681 tgatataagt ggtcgctata cgaataaata aaactacaaa ggcataaata tagagttatt
     1741 catgaaaggc ttgatgctcg gattttcggt tgcagtacct gtgaggccta taggattact
     1801 cacaattcgg cgcaccttgt caagaggtgt aatctctagt tgtgtgactg gactgggtgc
     1861 tgctacgcta gctcttatga ccacaggctt aaaatcttaa caaaaatgcc catgagcgat
     1921 acaggtttat tcgaatcaag catcaaaaaa agcaataaat gatcatcttt tgacacgatc
     1981 atgtagtgtt tttttgctgc acgaaagtat ttattaccat aaataacttt cgtgttccaa
     2041 gtgaaaaaat tctttccatc cctttcgtta tcggccattc tccacctgtt tcttttactt
     2101 atggttggag ttctgattta tatcctcatt ctcagtttta ccggctaaca taaaatctct
     2161 gaactacatt caaatgttgt atcgtttgta ataggctaca ttcagatgta gttctactca
     2221 tctgtttttc tttttatcct atcgtgtacg tgatatccaa aatcagtttc tacataaaaa
     2281 aggttcatca ttatgaaaat gataacatgg ggcattttgc tttgtctaat gacggcttgt
     2341 ggtagtggtg gcactggcaa tgaagattta tctaaggaag ctcaacaagc tttcgccgag
     2401 ggaaattacg aggacgcgtt aaatattttc ttgagtctcg ctgaatcaga accggtgacg
     2461 ggttataccg gtgccggatg gagtgcggtg aggttgcatg actacgcaaa tgccaacgtt
     2521 tatttccaga ccattgccgc ctccaacgat gccgatgcgt atgccggctg gagccttacg
     2581 gggtgggcgc tgtcggattt taattccgcg atacagcgcg cggatactgt gctttccaag
     2641 gacgctgatt ttgtgctaaa gttggatgac ggcgttgcgg cggcggatct gatattggtt
     2701 caggcattgt gttattatga aaccaataat ttttctatga cgcttcaaaa aataaagttg
     2761 atagacagcg ggttcaatgc cgacctcaat ggctcagata ttgcgcaggt attggcggct
     2821 aagctggagc agttatccgt tctattttca aaaacgttgg cgaaagggca ataatcatga
     2881 atttttttac aatacataag cacaatacga tacgtataat aatctcgagt gtggtgattt
     2941 tattttttgc ttcgacgctg catgcacaga gccgggtttc ttttttgaca ggggaattcg
     3001 aaaaaagtgg tcgcgagacc aacgaagcct ttctgcgtcg tattctgaga gcatcggtgg
     3061 aaatatcgtt taagaggttg agccgtgaaa ccgaccgcta cggtaaaaca cacgatggtt
     3121 tccaaatgat gtaccgttca gttcccgtgg aagatgccat catcaaagtg cactccgacc
     3181 accggggtat ctatgccgtc accggcgaaa cagctttacc ggcagacatt gcgattacac
     3241 ccaaaataga agaggcgcaa gcgctatcca aagcaacagg acatatcggc gcccaagcgt
     3301 acaaatggca ggatgcagat gaagaaaaaa tattaaaaga agttactgat aatcctcagg
     3361 cgagctttta tccgaaagca gaaacggttg ttgttaatat cgccaaagaa ggtgaaagcc
     3421 cggattttcg gttagcttac aaatttgata tttatgcgtc caaaccggtg agccgcgatt
     3481 gggtgtatgt ggatgcatcc tcgggcgaag tggttcgaaa aattaatcgg atttatcatg
     3541 gaacggcaac cggaacggcc gatacaagat atgcaggaac acgcacgatc aaaaccgatt
     3601 cgatcgcgcc caatcagttt cggctcaaag atctgacccg cggtaatggt gttatcacct
     3661 ataatctcaa tcacagtgtc aatgatgcgg actcgacgca tttcgtagac aacgacaatc
     3721 aatggaccgc tgctgaatat cataacggta actcggatga tggtgcgctt gatgcgcact
     3781 ggggagccga agtagtatat gattactgga aaaatgttca taaccaaagc agttgggacg
     3841 gcaataatgc gccaatgatc aattttgtgc attacaataa taataacccg aatgcttttt
     3901 gggatggata cagtgtgcac tatggccaat tccaatcggt caacccctgg acagaattag
     3961 acgtggtagg ccatgaattt ggtcacggaa tcatgatggg tatcgcgtcc cttaattata
     4021 gcggagaggg tacaagtttg cacgaaggtt gtgccgatat ctggggagcg cttgttgaca
     4081 attatggaaa tcaaaaattc ggattgaatc gagatccttg ggttttctca gaaaaaatcg
     4141 cattcagtcg ttcgtttaat tatttgatga cttataaatc gatcgattgg gattttgcac
     4201 acaacagccc gcatggtaac gggcaagttg cgcggtattg gttttatctc cttgccatgg
     4261 gcggatctgg agtgaataag tttggttctt cattcgcagt aaacccaatc ggtattgccg
     4321 aagcagaaaa gatcttttat caggctttta agcactatgt aacaccaaat agtattttta
     4381 ccgattttag aaaagctaca gaattggcag cacaggacat caatccggca cacgttgccg
     4441 ttgtccaatc cgcatgggac gctgtcgctg ttcccggaac aactggcagt gtcataggtt
     4501 gtacaccgtc gggaactcaa caaaacaaca ttcaagtgga ttatgttacc atcgccaata
     4561 aaggcgaacc cttttataat gcgggttggt cgtctggcgg atacatcaaa aaagattata
     4621 tgtttaacct gatccttggc aaaaaagtaa aaatggttat ttcatatccc gatgcatcga
     4681 cgaagcatta tgcaatgtgg atggataccg atagagatgg ccagtttgag gaaccttctg
     4741 aacgattagc cggcggaata atttcagcaa cctcatcgac ggattccatt caattgccac
     4801 aaacgctcgg aggtacgttt tcgtatggtg ctgtgccgat acgtatagcc gtgagtgcga
     4861 atccaatcaa cggcgcatgt gacaatgtcg tcggtgaagt cgaagactat tccgcatact
     4921 tgaatccgcc acaagtttta gacggttacc acttaaatat cggcgccaat tccggtgcag
     4981 aacttattgg gaaatcgaag ttgggttctt tggaaacgtc ggttttgttt tatccaagtg
     5041 acggattcaa aaatttagcg ggttctatat tcttgaacga ccccggttat accatgtggg
     5101 gttttgccgg tgaatcgtat ccgattatct tgaggagcca caaatttgtg ccctcagccc
     5161 acacagaata ttattcagta tggattgact acgataccaa taaggtattt gattcgaacg
     5221 aattagtatt ccaaggaagt ggtcaggatt cgattaaagg atatttaaaa attcctcaaa
     5281 atatgacaaa tcgggttacc cgtatgcgca tactcatgag tcgcaatgca tctataaccg
     5341 atccttatac ggctccggat tttggcgaag tagcggattt taatgttaaa ctttttgcca
     5401 atcctctgac ttccgacggc tacaaagcgc cacgaggtaa ttgttggcat acttatagta
     5461 cggatcctac gtacatgatg tccgattcca ttaccatcgg ttcattgatt ctgaaaaccg
     5521 gtcgcagcaa agatggcgga tatcatgatt tgaaaggaac atccgtattg ttcgtgccgt
     5581 taaatacaac tcaggccgcg gtgcgattga ctccattcat tgatcccaat ctgcaaccgg
     5641 tagattcggt atactacaat atctgggtag attacaatgg tgacaaagat tttaatgatg
     5701 tgggtgagca tgcatttttt cattacggga aatcaaaagc cgacggggtt ttaacgatac
     5761 cggccggcct tgccgctggc ttaagtacac gcatgcgttt gagcgtcagc tactttaccg
     5821 accacgtcca tgccgattcg gtggttaatt acggacaagt gatagatttg ggatttggca
     5881 tacaggcacc gttcacacgt tcaacgccgt tagccgatgt gaatgtgaac gaagatttcg
     5941 gcacgacaac cgcggcaata ctaaagaacg atcttattgg taacaccacc ggactttctt
     6001 ttactgcggc agctttatcc ggaggtgtga ccgtcaatcg gagcgtggat accgttcgta
     6061 tcgtgagcgt agccgatttt aacggtcaag tcaaaatcgc gatgaccact tcggatggta
     6121 gctacacact atacgatacg atcaccgtta cggtgattcc cgtcaatgat gcaccatatg
     6181 ttagtgcgga tgttcgtgat acgatgctta acgaggacgc agcgaaaacg tttttgatgc
     6241 gtatgagtga tcatttttcg gatgtggatc aggatgaatt tgcagtccaa tccaacgtgt
     6301 tgtccggtag tgcagtagta accattagta atgattcctt gtatttcaag ccgacggcaa
     6361 atttcaatgg aaatgtgatc gtcgggctca tggcatcgga tggtagtctg tccgtatcag
     6421 atacctttgc cgtgcaggtg cttcctgtcc aagacgcacc gtatcgtgtt gtaacattgc
     6481 gcgatacatc atttaatgag gatgtgagta atcagttggt tactaaactc acgccgtatt
     6541 tttcagatcc ggacaatgat cttctgacgt acaatgtatt agtactatcc ggtgtcgtat
     6601 cggcacgcgt aggtcaggac tctctgtaca ttactgcaca agccgatgct acgggtccaa
     6661 gctcactgcg catatcggcc acagatggaa atacatccgt gtatgatacg atcaccgtta
     6721 cggtgattcc cgtcaatgat gcaccatatg ttagtgcgga tgttcgtgat acgatgctta
     6781 acgaggacgc agcgaaaacg tttttgatgc gtatgagtga tcatttttcg gatgtggatc
     6841 aggatgaatt tgcagtccaa tccaacgtgt tgtccggtag tgcagtagta accattagta
     6901 atgattcctt gtatttcaag ccgacggcaa atttcaatgg aaatgtgatc gtcgggctca
     6961 tggcatcgga tggtagtctg tccgtatcag atacctttgc cgtgcaggtg cttcctgtcc
     7021 aagacgcacc gtatcgtgtt gtaacattgc gcgatacatc atttaatgag gatgtgagta
     7081 atcagttggt tactaaactc acgccgtatt tttcagatcc ggacaatgat cttctgacgt
     7141 acaatgtatt agtactatcc ggtgtcgtat cggcacgcgt aggtcaggac tctctgtaca
     7201 ttactgcaca agccgatgct acgggtccaa gctcactgcg catatcggcc acagatggaa
     7261 atacatccgt gtatgatacg atcactgtta cggtgattcc cgtcaatgat gcgccgaagc
     7321 tagtcgtatc attgcgcgat acgacactga ctcagaattt tacgcgggtg ctggtcgaaa
     7381 agctgaccga taattttacc gacgtggata atggtacgct acagtattcg gtcacatcgc
     7441 tgacgagcgg gctgaccgct tcattgggca atgactcaat ctatgttacc ggttctaatc
     7501 tggctatcgg tacggctaaa atcaccgtac aagccagtga cggatcgtta acggccaggg
     7561 acacattaca gataaccata atagatgtga ccgcgccatc gggttttgtt gcggctcttg
     7621 ccacgccgct tttgccgcgc gttcgtttta cgatcggcgg taatgaaata ttgagtagcg
     7681 cctcattaac ggtcaataat acgccggtca cgcttaccaa gcgggacagc aaacgttatt
     7741 ccggtcaata cgatctcacg acactgccga cgtcactcgt tgttgtaggc acggtcacgg
     7801 atttgggcaa taacgtgagc ccggcgatca atcgtacgat tcaggtgagt acgctcagca
     7861 aaccgctggc gatcaatggt ttcgaactcg cacgggcttc aggggtaaaa gagagtatcg
     7921 tactggtaag cgaagaagaa atcggtatga ataaatctga tgtaacaata caagccgtga
     7981 cgatcagttt tgatgaaacg atgacgattc gtctgtccgc gcctgtaaag catcgggaca
     8041 aaggtcggta tcgcatcgtg cgatgggcgg atgaagaaaa atatcccgac tattcgctga
     8101 tttctacttg gcaggaatgc acgatacaat accaaactgg cgtggtcata acgacgatag
     8161 aaaagtccgg cacgtatgtg ttaatctacg attccgatgc cgaggtattg ccgacggagt
     8221 ttgtactcaa tcaaaattat cccaacccgt ttaacccgac gacgacgatt cgttacgata
     8281 tagcggtgtc ggggcaagtg tcgcttatcg tctataatct gctcgggcaa aaggtgcgta
     8341 cgctggtcaa tggtttcgcg acgcaaggga cgtatcgttt gcaatgggat ggtaataatg
     8401 atatgggcca acgcgtttca tccggtgtgt acctttaccg tctggaatcc ggaggaaaac
     8461 gattcactaa gcgaatgatg ttcttgaagt aaaaacaatc ctcacttgat gagaaccggc
     8521 ggcgtgaacg ggtagtaacc tcagtaacgc cgttttttta tgcatgaata gcaatgatca
     8581 aaccacattg tcatgcagta gtgtttcgaa aaaattaact ctaaattatc agagcaaatt
     8641 ttatgaaaat agcaaaataa gcaggcaaca tggatctaca atcaccgatg atacagacga
     8701 tactggttat tgcctcgggt ctcgtgatga taggattgat actgacgctg attgttagtt
     8761 ttaccggata agttgagtta tgtgtgacta atttaccgaa atacccaaat ccgtttccat
     8821 catcgcgaca cgttttaata tttgcgtgat caaatccatc caagcatcat gtatagcgtc
     8881 ggaacaatga ttatccgacg actcttttat agccgataat aaagcgctcc ccacgttacg
     8941 aaaattgact acatgtaatc ccagcatgtg acccttttct atcacagcat acaattcatg
     9001 ccaagcgcct tgggcgttgg tcgcattttt gatcaggagc ttcataagat gatcaaaatg
     9061 tttttgttgt tgatctgagc agaacggcgt gaggtgtaaa aagcgctcat ccaatacggt
     9121 caatcgatta aaaaatagag acggtacgtt ttttttctct gcaaatcgcg cgtagctgga
     9181 caaaattctt aatactttat cttcggatgt caggagagcg gaggatagta ccatattttt
     9241 ttcgatgaga taattaatgg gataaaaata cgtttctttg ggaaaagtat aaaaaaagtg
     9301 atgcgatcgg caaactacat gaatatgcag gactgcaaat cccggatgcg gaacatgaat
     9361 ataaaaatca tcaaactatt cccggctggc tcttgcactt ttgatgcctg cgtattatgt
     9421 tgtcacgttg tggtataata ctacagcaaa agtcaagtta ctcattcttg gaaaggtgtt
     9481 aatgctatat ggccgctgaa tcggtcttga atctggacga agtaacgatc cgcttagccg
     9541 gtgactccgg tgatggtatg cagcttgtcg gtacggaatt gacgaatgca tcggcgttgc
     9601 tcggtaacga tgtcggtaca ctgccggatt accccgcgga aattcgcgca cctgccggta
     9661 cattgcccgg tgtgagcggt ttccaattgc acatcggaag ttttgatatc catacgcccg
     9721 gcgatgaagt ggacgtccta gtcgcgatga atcctgccgc gctcaaagcc aatatcagat
     9781 ctctcaaacc caacggcgta ttgatcgtca atgcggaatc gtttggcgaa cgcgatctga
     9841 aactggccaa ctgtgcgacc aatccgctcg aagacggtac ggtggacggt tatcagcttt
     9901 ttagagtgga tatgtcgtct cataccaaag cggcgcttaa agatacgggg cttgatcaca
     9961 aatccatgga gcgttgcaaa aacttttttt cactcggcat gatcgcttgg ttgtacaatc
    10021 gtccgatgga cggtacgatc aaatttctta aagaaaaatt tgccaaaaaa cctttactcg
    10081 ccgagtccaa tatcctcgct atgaaagcgg gttatgcgta ttgcgaagcg acagaagctt
    10141 ttgctaattc gtatgaaatc cctccggcca agttacccgt cggaaaatat cgcaatatca
    10201 gcggaaacga agcgattgct ttaggttttg tagcggcttc tcaaaaatcg ggattggaat
    10261 tgtttttagg ttcgtatccg atcacgccgg ccagcgatat tctgcacggc cttgcgaagt
    10321 ataaaaattt tggcgttaaa acgtttcagg ccgaagatga aatcgcggcg atatgcgcag
    10381 cgatcggcgc ttcatttgcc ggtgaactcg gcatcacgag tacgtccggc cccggtgtct
    10441 gtctcaaaag cgaagctata ggacttgccg tgatgaccga gcttccgctg gtggtgatca
    10501 atgtgcaacg cgccggtccg agcacagggc ttcctaccaa aaccgaacag tccgatcttt
    10561 tgcaagcgat gtttggacgt aatggcgaat cgcccgtgcc cattattgcc gcctcgcgtc
    10621 cggccgattg ttttgaatgc gcctacgaag cagcacgtat cgcggtcaaa tatatgacgc
    10681 cggtatttct gctcgctgac ggatatatcg gtaacggcgc cgaaccgtgg ctcattcccg
    10741 attccgataa actcaacgaa ttttcagcgc atcggacatt taccggtgaa aagtttacgc
    10801 cgtatgctcg tgatgaaaaa acgcttgcac gtccgtggat tgtaccgggt acgccgggat
    10861 tcgagcatcg catcggcggc ttggagaaaa aaaatatcgc cggcacggtc aattacgaac
    10921 cggaaaatca tgattatatg gtgcgcattc gcggcgaaaa agtgaaacgt atcgcgcagg
    10981 agtggaatcc gttatcaata gacggaccgg actcgggtga tcttttggtg atcggttggg
    11041 gcggcaccta cggcgcaatt cgtacagccg ttgatcgaag ccgtgaaaaa ggattgagcg
    11101 tgtcccgtat tcatctgcgt tggatcaatc cgtttccgca agatcttgac gcgattctca
    11161 aacgattcaa acacgtgttg attcctgaaa tcaatctcgg tcaactccgc atgctgatcc
    11221 gtgcggaata cctcgtggat gcgatcgggc tcaataaggt gaccggccag ccttttagtg
    11281 tacgtgaaat cgaaaataaa attacggaaa ttttaaagaa ataatttttc tgtccgattg
    11341 ttgttaataa cgtctcggcg cagagctaag aaaaagaagc aggtttatac gatggtacag
    11401 gatatcaaac ccgtttacgc aaaaaaagat tttgaatccg atcaggaaat tcgctggtgc
    11461 ccgggatgcg gcgactactc catcttggca caagtacagc gtaccttgcc ggaatttcag
    11521 acacaaaaag aaaaatatgt atttgtgtcg ggtatcggtt gctccagccg gtttccgtat
    11581 tatatgaata cctacgggtt tcactcgatt cacggacgtg cgcccgcgat tgcgaccggc
    11641 atcaaagccg ccaatccgga actgaccgtg ttcgtcatca gtggcgacgg agattcgctt
    11701 tccatcggcg gtaaccactt cctgcacgtt ttgcgtcgta atttggatat caattatctg
    11761 ctgtttaata accggattta tggattgacc aaaggtcaat attcgccgac ttccgaaacg
    11821 ggcaaaatta ccaaatccac gccgatgggc tcactggaac atccccttaa tccgttatcc
    11881 gtagcactcg gtgccggagc gacattcgta gcgcgcgcga tggatcgtga tcccaaagga
    11941 cttcaggatg tgattcgcgc tgcgatcaaa caccgcggta cgtcgttcgt cgaagtgtac
    12001 caaaattgca atatattcaa cgacggcgcg tttgagcatt taacggaaaa agataccaaa
    12061 cccgataata cgatatggct tgagcatggc aaaccgcttg tgtttggcgc caaaaaaaat
    12121 aaagggatcc gccttgacgg attcaaacct gttgttgtca atctggaaga cggttcgagc
    12181 tctgtcaatg atctgcttgt gcacgacatg cacaatacgg atccggtgat ggcatttata
    12241 tacagccaga tgaccgaagc gccgggactt cccacaccga tcggcgtttt ccggagcata
    12301 caaaaaagtt gttatgagga agatatgcgt actcagatcg ccttggccaa agacaaaatg
    12361 ggacccggtg atctgaaaaa acttttgcat aaaggtgata cgtggacggt cgaataacga
    12421 cccccgtggg ctttagggat tttgaaaagg agagttggtc atgaaaaaag gaaaaattag
    12481 cataacgtta gccggtcttt gttttttgtc gtttaccggt tgcggcaaat cggaatgggt
    12541 gacattttcc gatactaaaa aagatcaaat cacttccatc gtcgagagcg gtacgtccat
    12601 ttggtatggt acgcgcaccg gtcttgtaca gtttgacaaa gccaccgaaa agcgtaaagt
    12661 ctataccgtc aataacggcc tgccggataa cgatatcaaa tatgtgaccc gcgacgccaa
    12721 aggtaaaata tggatcggca cgaaccttgg attgtcttct tttgacggtc aaaactggga
    12781 cgtttattcc accgccgaat cgaaattgcc cggcgatttg atc
//
LOCUS       DAOQNQ010000062        12766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1739_length_12766_cov_5.756982, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000062 DAOQNQ010000000
VERSION     DAOQNQ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12766
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1739_length_12766_cov_5.756982"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="PK754_05805"
     CDS             <1..1279
                     /locus_tag="PK754_05805"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNB09050.1"
                     /translation="YLAMIESGYSAQARSHAHAVGHWQFVKKTATGYGMNVDWWVDDR
                     RDLYVSTRYAFKYLKDLFNEFGDWYLALAAYNAGSGRIYNAMKKNNTRDYWEMTKLPR
                     ETRNYVPYFIAATQIVRDPAKYGIALETESAARMDTVTVTECLDLEIIAQCVNSTPDS
                     IRDLNPALLRWCTPPTVPNYLLKIPFGTRTAFLENYAKIPQDKKRSWVRYQVKYGETL
                     SGIANRYGTDVKAIQQANQLKSSVNLTTGQWILIPVAPKSYVATKEPTPVRNTPVINA
                     SNQAKTTNAGGTGKTNTNWMVGKKKLTFKVEAGQTLGHIAEWYNITAQNIRDWNGLYY
                     GDPIFPGQVLTLYIDQYMPDEGYRNATNKRTKEVTAEEPGMKIYVVQDNDNLISIAKM
                     FNVEVSNIKRWNKLSSNTIKVGDKLKIYLNDKQ"
     gene            complement(1338..1925)
                     /locus_tag="PK754_05810"
     CDS             complement(1338..1925)
                     /locus_tag="PK754_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09051.1"
                     /translation="MKRIIYSLLVMLAAVSCDVDVEDTSPNSYAEFMSEGWILFSQTY
                     YDSSYHTFADAIKKYPDSADVYTGAGWALMKMDSLNKAGQTFITGSTKVGTDVDFYAG
                     WSFCENARRQFSSSNEKLMTALSLKPTWSFPYAGNIGASDLKAMKAENYFMMGDFGSS
                     LAAVQVLDPNFTADIQTDTGIGELAVKIEALKKAN"
     gene            complement(1937..4768)
                     /locus_tag="PK754_05815"
     CDS             complement(1937..4768)
                     /locus_tag="PK754_05815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HNB09052.1"
                     /translation="MQKLYVAGLVFLLSYSSGVIIAQTNMTFLSQKDDHGSNYANLWG
                     YVDSLGNEYAIVGCFNGTAIINVTNPTSPTEIAYIPAPSSSWHEVQVWDKYAYVVSEG
                     GNGLQVIDLSQLPTSASLVQNWTTPNYFRTHALQVRNGYAYLTGGNVTQGVPGGKDIG
                     GIKIVSLADPRNPVEVGNWAENYVHDCYVRNDTIYAASIYQGIIYVIDTKVKSKPRTI
                     HQFTYPNAFSHNTALSDDGNYLFTTDETSSPAGRLRIWNISTLRDGIPNNTNISQVKD
                     FGTTAIVHNVYVKGRYAYASYYTEGVRIWDLNNISTLPVAGAYDTYDADNSANFTGAW
                     GVFPFFPSGNIAISDIVRGLIMISFSGKETGQISGTVKDVNTDLPLTGVKVALLEDTQ
                     TRLTNALGQYNFSALHGATTVKLRKPGYRTAYHYTTVPLNATSTYNFSLTPLTAKSPT
                     SISAFADSALVDLVWNSPDPNDIVKYRIYYGMSPNPTTQMDSTIDSRDTTRSISTLTV
                     GGTYYFRVKAVYWDGQVSDFSTETSALIPNKKPVATTILNILSDSAQATIRWRKSNDS
                     DFLRYRIFGGTSPNPTTQIDSISAQNDTVKTITNLMNGQTYYFAVSVVDVDGLMSRLS
                     NEVSVLVKPIVFNLVTALYQNSVLPQYINVVVNTNIALVSPPSVRFWKTNDTTSVSLS
                     VLPGTANTYSGSYQLKQSGTYTIQTRGISFRNQDSTKLRYLTATLAKPSSDNRLTTED
                     GASVLMIPGSAVTTDMFFTCSIEKNEETERIYAFGPDINYTVPLQLQFKLPALTNEQS
                     KKWFILREENGQWVKIESHVLANENIIRTNLSSLGRFKLQYDPTSGDETLIVKSFDLK
                     QNFPNPFNPETTIAFTLENNSDRLTLTVYNTLGQKIKTLWDGPKTSGYHRVTWDGTNE
                     TGQRVSTGTYIYRVNTGKSVFTKKMLLIK"
     gene            complement(4804..6051)
                     /locus_tag="PK754_05820"
     CDS             complement(4804..6051)
                     /locus_tag="PK754_05820"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HNB09053.1"
                     /translation="MIILKKIEHYKGELSLGIMVTGLLSCFFIAASYPHFLPMLVFIL
                     QSGFEAGTVGGAADWLAVKMIFDEIKIGRFRIVPASGIIPRKQKSIADGAGKLVAEEW
                     LSKDSITGWLSSFDAASALAVQLKKIEQNGELDRFINWARARIIQWLDSPESNSYFEM
                     LLREGLKSIKPIHFLGHSVPDEQFTKILHRLLDHAPEKIADTMTSQTVYELLRDNIER
                     EQGFFSKLFFDAGELTERIITKATSILRSIAADENHELRLEIIKRSMAWKQTLKNQDN
                     NTWDTWIQEIISETDLKQYIPAIKVKLKSLLDSETDSGKKIDELLKNIWHNTIFMLES
                     DRSLQIAVNTRFIEIASDMLQAHHGKIAELVSYNINKLSVQEIRDQFRSRTYDDMQWI
                     RVNGAVAGFAIGILIGLFRLIIG"
     gene            complement(6057..6683)
                     /gene="tmk"
                     /locus_tag="PK754_05825"
     CDS             complement(6057..6683)
                     /gene="tmk"
                     /locus_tag="PK754_05825"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNB09054.1"
                     /translation="MNRGIFISFEGIDGCGKSTQIRRLQMRIEAEGLPVIAMRDPGGT
                     TVSEKIRQILLSTDSVIDPVTELLLYEAARAQILAEKIRPSLAEGYVVLIDRFFDSTT
                     AYQGYGRKIDLALIHAANHIATQGLSPDRTYLIDISWDESQKRRIASQKDRMENEGRD
                     FFECVRQGYLALAEKESGRVFRLDGDQNIDRLSNLIYQDFLTLRDRTK"
     gene            complement(6680..7429)
                     /locus_tag="PK754_05830"
     CDS             complement(6680..7429)
                     /locus_tag="PK754_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNB09055.1"
                     /translation="MIDEALIRRLQQAKRWVFFTGAGMSAESGVPTFRDAQSGLWAHF
                     KPEELATPQAFKRNPKMVWEWYAMRRQMIAAAQPNAGHYVISELEKHFEVTTITQNID
                     NLHTVAGSTRVLELHGNIFRTKCFTDDKIVEHPLDTDDIPPLCPRCCGYVRPDVVWFG
                     ELLPEKIFEQSMHAASNTDVFLCIGTSGVVYPAAGLPIHARQSGALLIDINPETTPIS
                     EQAAYHIQGTSGPILTQLFEKVTGHFMESRT"
     gene            complement(7429..8889)
                     /locus_tag="PK754_05835"
     CDS             complement(7429..8889)
                     /locus_tag="PK754_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HNB09056.1"
                     /translation="MSDHVLKDLEPKRVFDYFYEITQVPRPSKKEEKIRAYIKGVAQK
                     LNSDWSEDAAGNIVIRKPAAPGYENKPVVVLQGHLDMVCQKTPDTQIDFDNDPIKPRI
                     DGEWVKATGTTLGADNGIAVATMLAILEDTTMKHGPLELLFTVDEETGLTGALKLEPS
                     MLKGRYLLNLDSEDEGVLFIGCSGGKDGVIHFPIKRKPAKENETRCLIAVEGGVGGHS
                     GMDIILGRANANKILTRVLYELTKEYKFKLSDIKGGTVRNAIPRDAAVHIAVRKEDIG
                     AFKVRVQKMHNYLSAKYQSTDPDLKIVFKEAPIAQFSTIKRSRSVELIRLLKSLPNGV
                     QAMSKDVDGLVETSVNLATIQTHEKEIIIGSSHRSAIEEEKRDILDQVGAICSLTHAT
                     VKETDGYPGWKPNPQSKLLAITKKVFKEIYGKNPKVTAIHAGLECGIIGEKFTDMDMI
                     SFGPTLRNVHSPDEGLHIPTVKPFYTAVQKLLETIA"
     gene            9019..9681
                     /locus_tag="PK754_05840"
     CDS             9019..9681
                     /locus_tag="PK754_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB09057.1"
                     /translation="MKLKKILIVDNDPASLVNLSAMVEKAGFTPVGFQKPTLAMEFIE
                     KPDNHDIRMIIAEMNIPGSDGFTFLKAIQKKPQLKLMPFLFLSELQDTAVLVNAYEQG
                     AVDYFIKPIRKESLFIAKIKSMVDAFERTLQSANTLLSGQLSDKPLVELLAVCENESL
                     NGYLRIKHADGIGVITFVKGLPEKMAIVDAQQKIIKSESEAFEAMSDWSEGEFLIRRG
                     NL"
     gene            complement(9692..10666)
                     /gene="meaB"
                     /locus_tag="PK754_05845"
     CDS             complement(9692..10666)
                     /gene="meaB"
                     /locus_tag="PK754_05845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNB09058.1"
                     /translation="MEHIDAVRLLAHNDPKRKRYLSRLLSSVENDAENGPDILRALLP
                     YTGQAYRIGITGPPGAGKSTLVNAAALGLAQQGKRVGIIAVDPTSAFTGGAILGDRFR
                     MQAISTHENIFIRSMATRGAMGGLVHTAHAAADLLDAFGYDVIIFETVGVGQSEIDIV
                     QYTDTVVVVLVPESGDGVQAMKAGIMEIADLFVVNKSDRDQADILKRELEAAVQIRYT
                     HAPWMPVVLKTVATQNEGIDAVLETVFKHREYLESSGDMDIRRKSRIRNALLRGVNKK
                     LHDHLTSEGYRGLVDEQIDAVFYKKIILQDAADMIFRSMYSSAIPPGK"
     gene            complement(10682..11539)
                     /locus_tag="PK754_05850"
     CDS             complement(10682..11539)
                     /locus_tag="PK754_05850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB09059.1"
                     /translation="MQTPHIFVIILSYNGRHLTEACVRSCLDSDFTSFTIVLVDNASP
                     DGSGDYLKEKFNSEIHSGKIKYIGNEANLGFAGGNNVGIQYALNNNADAVLLLNNDTV
                     MDKKCLVHLSEGLAHYPQAGILGPKIYYFEPNDQIWFAGAEFKLYKGTARHIGIREKE
                     NLQYEEAKTSDYITGCAMLIRCDVFKSVPGLDTSYHMYSEDVDFCFRAKQAGFELVYI
                     PQAKIWHKISASTGGQLSLRKIRLRFFSNMKLLIKYARWYHWLTIPFYFFADLMRIAF
                     RILTGAIRN"
     gene            complement(11552..>12766)
                     /locus_tag="PK754_05855"
     CDS             complement(11552..>12766)
                     /locus_tag="PK754_05855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB09060.1"
                     /translation="TEHLMSSDEYKLTTLASSALFKNIPEDALRRLLPHFTLKVYPTN
                     TVIFEDGNTQPDGMYIIAKGFVKIFKSVPGEEVRQQAVAVLPAGSYFGEMALLDEEVR
                     SAGAVTMYETELLFFPKESFHALMHEDIKLSHEIMTRLAKVMSKRLRDTNNLFREVVS
                     WGYRARKEVRDLKSNFLSTISHELRTPIHSIQGFSTLMKESKDVDDETKQRFVEVILH
                     ESKRLADLINDLISLAEIEYGSIIIERHPNNLKELLSKAFDEYKDIAREKNINLEMKV
                     EDTFPIVAMDGPRFIQAIGHLIENAIKFTGFGGKVGMHALMTTDAIEIHVHDTGSGIP
                     SKYVERIFDKFYQVDQTTTRSAEGAGIGLSLARTIVELHGGKIIVTSVEGKGSTFTVT
                     VPLSHIAFQDGR"
BASE COUNT         3558 a         2882 c         2633 g         3693 t
ORIGIN      
        1 ataccttgcg atgatcgaaa gcggatatag cgcacaggcg cgttcgcacg cgcacgctgt
       61 cgggcattgg cagtttgtca aaaaaactgc taccggttat gggatgaatg ttgactggtg
      121 ggttgacgat cgtcgggact tatacgtttc cacgcgttat gctttcaaat acctcaagga
      181 tttgttcaat gaatttggcg actggtattt ggcattagcc gcttataacg ccggatcggg
      241 tcgtatatat aatgcgatga agaaaaataa tacgcgcgat tattgggaaa tgaccaaatt
      301 gcctagggaa acacgtaact atgttcccta ttttatagct gccacacaga tcgtccgcga
      361 cccagctaaa tacggtattg ccttggaaac cgaatcggct gcacgcatgg atacggtaac
      421 tgtgaccgaa tgtcttgatc tggagattat tgcgcaatgc gtcaactcga caccggacag
      481 tattcgcgac ctcaaccctg cacttcttcg ttggtgtaca cctccgacag ttccaaacta
      541 cttattgaaa attccctttg gtacgcgtac ggcctttctg gaaaattatg ccaaaattcc
      601 acaagacaag aaacgctcat gggtacgcta tcaggtcaag tatggtgaaa cgttgtccgg
      661 gattgccaat cgttatggga cggacgtcaa agccatacag caggccaatc agttaaaaag
      721 cagtgtcaac ctaacgacgg gccaatggat tcttattccg gtggcgccca aatcgtatgt
      781 agctaccaaa gaacctacac ctgtccgaaa tacaccggtg ataaatgctt ccaatcaagc
      841 gaagactact aatgcgggtg gtaccggtaa aaccaatacc aactggatgg ttggaaagaa
      901 aaaattgact tttaaagtcg aagcgggtca gacattaggt catattgccg agtggtataa
      961 catcaccgcg caaaatattc gcgactggaa cgggctttat tacggcgatc cgatattccc
     1021 cggccaggta ttgacccttt atattgatca gtatatgccg gacgagggtt atcgtaacgc
     1081 gaccaataaa cgcactaaag aagtgacggc cgaagagccg ggaatgaaaa tatatgtcgt
     1141 gcaggataac gataatctga tcagtattgc aaaaatgttt aatgtagaag tgtccaacat
     1201 caaacgatgg aataaacttt cttccaatac cattaaggtc ggcgacaagc taaaaatcta
     1261 cctcaatgac aaacaataag atacctgtct tctgcataga gcttgggttt ttattatcaa
     1321 agtcgtttga gtagatacta attagccttt ttcagcgctt ctattttaac cgccaattca
     1381 ccgatgccgg tatccgtttg tatatcggct gtgaaattag gatccagcac ttgtacggcg
     1441 gccagacttg atccaaaatc acccatcata aaataatttt ccgctttcat tgctttaaga
     1501 tccgaggctc cgatatttcc ggcatacgga aatgaccaag tcggcttaag gctgagcgcc
     1561 gtcattaatt tttcattaga acttgaaaac tgacggcgtg cattttcgca aaaagaccaa
     1621 ccggcataaa agtcaacatc cgtaccgact ttggttgatc ctgttataaa agtttgaccg
     1681 gctttattca aactgtccat tttcatcaat gcccatccgg cgcctgtgta aacatcggca
     1741 gaatccggat atttctttat ggcatcggca aatgtgtgat acgaagagtc gtaataagtc
     1801 tggctaaata aaatccatcc ttccgacata aattcggcat acgaattcgg cgatgtatct
     1861 tccacatcca catcgcacga cacagcggcg agcattacta ataatgaata tattatacgt
     1921 ttcatgtttt ttccccttat ttgatcagaa gcattttttt agtaaacacg gacttgcctg
     1981 tattcacacg gtatatatat gtacccgtac tcacccgttg cccggtttca ttggttccat
     2041 cccatgtaac gcgatggtag cctgaagttt tcggtccatc ccatagtgtt tttatttttt
     2101 ggccaagtgt attgtaaacc gttagtgtca acctatcaga attattttcc aaggtaaatg
     2161 ctatggtcgt ttccggatta aacggattgg gaaagttttg ttttagatca aatgatttca
     2221 caatcaacgt ttcgtcaccg ctcgtcggat cgtattgtaa tttaaatcga cctaacgagg
     2281 agaggtttgt tcttattata ttttcattgg ccagtacatg cgattctatt ttgacccact
     2341 gtccattttc ttcgcgaaga ataaaccatt ttttggactg ctcattagtc aaagcgggca
     2401 acttgaattg aagttgtagt ggcacagtat agttgatatc cggcccaaac gcgtaaattc
     2461 gttcggtttc ttcatttttt tctatcgaac atgtaaaaaa catatcggtt gtcacagcac
     2521 tacccggaat catcaatacg gaagccccgt cttcagtagt taaacggtta tccgaacttg
     2581 gtttggccaa agtagccgtg agataacgta attttgtaga atcttgattt ctgaatgaaa
     2641 tcccacgtgt ttggattgtg tacgttccgc tttgttttag ttgataactg ccagaatagg
     2701 tatttgccgt tccaggtaag acgcttagag ataccgatgt ggtatcgttg gttttccaaa
     2761 agcgaacgct tggaggacta accaaagcta tattggtgtt tacaacaacg tttatatatt
     2821 gcggaagcac tgagttttga tacaatgccg tcaccaaatt aaacacaata ggttttacta
     2881 agaccgaaac ttcatttgat aaacgactca ttaacccgtc cacatccact acactcacag
     2941 caaaataata tgtctgcccg ttcataagat tggtaattgt ttttaccgta tcattttggg
     3001 ccgaaatgct gtctatttgc gtagtgggat taggtgaagt tcctccaaat atacgatagc
     3061 gcagaaagtc gctatcgttg gatttacgcc aacgtattgt tgcttgagcg ctatcggata
     3121 atatatttag aatcgtcgtt gcaacaggtt ttttgttagg tattaatgcg ctggtttctg
     3181 tagaaaaatc actcacttgc ccatcccaat acacggcctt tactctgaaa taatatgtcc
     3241 ctcctacagt gagagtggaa attgagcgtg tcgtatcccg tgaatctatt gtagagtcca
     3301 tttgtgttgt aggattgggt gacatcccgt aatatattct gtatttgact atatcgttcg
     3361 gatcgggtga attccatact aaatcaacca gcgcactatc ggcaaacgcc gaaattgaag
     3421 tcggcgactt cgccgttagt ggtgtaagag aaaaattata cgttgaagta gcgtttaaag
     3481 gcaccgtcgt ataatgatat gcagtgcgat aacccggttt gcgcagtttt accgttgttg
     3541 caccgtgcaa tgccgaaaag ttatactgcc ccaaggcgtt cgtcaaacgt gtttgcgtat
     3601 cttcgagtaa ggccaccttt actccggtca aaggtaaatc cgtgtttaca tcttttacag
     3661 taccgctaat ttgaccggtt tctttgccgc taaaactgat cattatcaat ccacgcacta
     3721 tatcggagat agctatattc cctgacggga aaaaagggaa aacaccccag gcgccggtaa
     3781 aatttgccga attatccgca tcgtacgtat cgtatgctcc ggcgacaggc aaggtcgaaa
     3841 tattatttag atcccaaatt cgtacgcctt cggtataata ggaagcatat gcataacgac
     3901 ctttaacgta aacattgtgg acgatggccg tcgtaccaaa atctttgact tgcgatatat
     3961 tcgtattgtt tggaattcca tcgcgcagag ttgaaatatt ccaaattcgt aaacgacccg
     4021 ccgggcttga cgtttcgtcc gtagtgaaaa gataatttcc gtcatcagat aatgcagtat
     4081 tgtgactaaa tgcgttagga taagtaaact gatgaatggt gcgcggttta gattttactt
     4141 ttgtgtcaat gacataaata atgccctgat aaatcgaagc cgcgtagatc gtatcattgc
     4201 gtacataaca gtcatgtaca taattttcag cccagttccc cacttccaca ggatttctgg
     4261 gatcggctaa actgacgatt ttaattccgc cgatatcttt accgccggga acgccttgcg
     4321 tcacattacc accggtaagg tacgcgtatc catttcttac ctgcagcgca tgtgttctga
     4381 aataatttgg cgttgtccag ttttgaacta atgaggctga ggtcggcaat tgagataaat
     4441 caattacctg caaaccgtta ccgccttcag atacgacata agcatattta tcccaaacct
     4501 gaacttcatg ccatgaagaa cttggtgcag gaatgtaagc gatttcagtt ggagaggtgg
     4561 ggttggtaac attgatgatc gctgtaccgt tgaaacatcc cacgatagcg tattcgtttc
     4621 ccagcgagtc aacatacccc cagaggttag catagttgct tccgtgatcg tctttttggc
     4681 tcaaaaacgt catatttgtt tgtgcgatga tgacacctga ggaataactt aaaagaaaaa
     4741 ctaacccggc cacgtaaagt ttttgcataa cagaactttc gcctttgata ttgataacga
     4801 aatctatccg ataattaaac gaaacaaacc aataagaatt ccaatcgcaa aaccggctac
     4861 ggcgccattg acacggatcc actgcatatc atcatatgtt cgcgaacgga actgatcacg
     4921 tatctcctga acagataatt tattgatgtt ataactcacc aattctgcaa tcttaccgtg
     4981 atgcgcttgc agcatatcgg acgctatttc aataaaacgg gtgttgacgg ctatctgtaa
     5041 gctacgatct gattctaaca taaaaatggt attatgccaa atgtttttta acagttcatc
     5101 tatttttttt ccgctatccg tttcactatc caataatgat tttaatttta ctttgatcgc
     5161 cggtatgtat tgtttcaaat ctgtttcact tataatctct tgtatccatg tgtcccatgt
     5221 attgttgtct tgattcttaa gcgtttgttt ccatgccatg gaacgtttta ttatttcaag
     5281 gcgcaattca tgattttcat cggcggctat agatctcaat atagatgtcg ctttggtaat
     5341 gatgcgttca gttaattcgc cggcgtcaaa gaacagctta gaaaaaaaac cttgttctct
     5401 ttcaatatta tcgcgcaaga gttcatacac ggtttgtgaa gtcatggtat cggctatttt
     5461 ttcaggcgca tgatcaagca agcgatgaag aattttagta aattgttcgt ccggtacaga
     5521 gtgacccaaa aaatgtatcg gcttaatgct ttttaatcct tcgcgcaata acatttcaaa
     5581 atatgagttc gattccggcg aatccagcca ttgaataatt cgcgcacgtg cccaattgat
     5641 aaaacgatcc agctcaccat tctgctcgat tttttttaat tgaacagcta atgccgatgc
     5701 ggcatcaaat gagcttaacc atccggttat actgtctttg gataaccatt cttctgcgac
     5761 taatttacca gcgccgtcag cgatagattt ctgttttcgc ggaataatac cggaagccgg
     5821 cacaatacga aagcggccaa tcttgatttc atcaaaaatc atctttacag ctagccaatc
     5881 tgctgctccg cctactgtac cggcttcaaa accggattgc agaataaata ccaacatcgg
     5941 taaaaaatga ggatatgaag ccgcgataaa aaaacacgac agcagtccgg tcaccataat
     6001 acccagcgac aattcgcctt tgtagtgctc aatttttttt aaaataatca ttatggctat
     6061 ttcgttctat cgcggagagt aagaaaatct tgatatataa gattagatag acgatctata
     6121 ttttggtctc catccagacg gaatacgcgt ccggattctt tttcagcgag agctaagtat
     6181 ccttgacgaa cgcattcaaa aaaatcccgc ccttcgtttt ccattcgatc tttctgcgac
     6241 gcgatgcgcc gcttctggct ttcatcccat gaaatatcaa tcaggtatgt acgatccggt
     6301 gaaagacctt gggtagcgat atggttagct gcgtggatca gtgctaaatc aatttttcgc
     6361 ccatatcctt gataagccgt cgtcgaatca aaaaaacggt caattaacac gacatatcct
     6421 tcggccaacg aaggtctgat cttttccgct aagatctgag cacgcgccgc ttcgtagagc
     6481 aacaattccg taaccggatc aatgacgctg tcggtagata acaagatttg acgtattttt
     6541 tccgatacag tagtccctcc cggatcacgc atagcgatga ccggcagtcc ctctgcttcg
     6601 atacgcattt gcaaacgtcg aatctgggtg gatttaccgc agccgtcaat tccttcgaaa
     6661 gaaataaaaa taccacgatt catgtgcgtg attccataaa atggccggtt actttttcaa
     6721 atagttgtgt caagataggt cccgatgtgc cttgaatgtg gtatgcggct tgttcagaga
     6781 taggcgttgt ttcagggttt atatcaatca atagcgcccc cgattgtcgc gcatgtatcg
     6841 gcaacccggc cgccggatat acgacaccgc tggtaccgat gcataaaaaa acatccgtgt
     6901 ttgatgccgc atgcatagac tgttcaaaaa ttttttcggg taataattct ccaaaccaaa
     6961 ccacgtccgg ccttacatag ccacagcatc tgggacatag cggtggtata tcatctgtat
     7021 ccaaaggatg ctccacaatc ttatcatccg taaagcactt agttcgaaaa atattaccat
     7081 gaagttccaa tacacgtgta ctgccggcta cggtgtgtaa gttatctata ttttgggtta
     7141 ttgtcgtcac ttcaaaatgt ttttccagtt cggatatcac gtaatgaccc gcgttgggtt
     7201 gagccgctgc gatcatttga cgtcgcatcg cgtaccattc ccataccatt ttgggattac
     7261 gtttaaatgc ctggggggtg gctaattctt ccggtttaaa atgcgcccat agaccgcttt
     7321 gtgcgtcccg aaatgtcgga acaccgctct cggcggacat cccggcgccc gtgaaaaaaa
     7381 cccaacgctt agcttgttgc agacgacgaa taagcgcttc atctatcatt acgcgatggt
     7441 ctccaacagc ttttgtaccg cagtataaaa tggttttacg gtcggtatgt ggagaccttc
     7501 atccggcgag tgaacgttac gaagggtcgg accaaaagaa atcatatcca tatcggtaaa
     7561 tttttcaccg ataataccgc actccaaacc ggcgtgaata gccgttactt tcggattttt
     7621 accgtaaatt tctttgaaga cttttttggt tatagccaga agcttcgact gtggattggg
     7681 tttccatccc ggataaccgt cggtttcttt caccgttgca tgcgtcaacg aacaaatggc
     7741 gccaacctga tctaaaatgt cgcgtttttc ttcttcgatc gcactgcgat gactggaacc
     7801 tattatgatc tctttttcgt gtgtttgtat ggttgcaaga ttaacagatg tttcgacaag
     7861 accatcaaca tctttactca tagcttgtac gccgttgggc aatgatttca aaagtcgaat
     7921 cagttcaacg gaacgcgaac gtttaatcgt actaaactga gcaatgggcg cttctttgaa
     7981 aacaattttt agatccggat cggtactctg atatttcgca ctgaggtaat tatgcatttt
     8041 ttgaacgcgc actttgaacg caccgatatc ttccttgcgt acggctatat gcacagcggc
     8101 gtcacgcgga atggcattac gtacggttcc gcctttgatg tccgataatt taaatttgta
     8161 ctctttggtc aattcgtaca acacgcgcgt caaaatttta ttagcattag cccggcccaa
     8221 aataatatcc ataccggaat gcccgccgac accaccttca acggcgataa gacagcgtgt
     8281 ttcattttct ttagccggtt tacgtttgat cggaaaatga ataacaccgt ccttcccccc
     8341 tgaacaaccg atgaatagca caccttcatc ttccgaatcg agattgagca aataacgccc
     8401 tttgagcatg gaaggctcca atttcaaagc gccggtaagg cctgtttctt catcaacggt
     8461 aaatagtaat tctaacggcc catgtttcat ggtcgtgtct tcaagtatgg cgagcattgt
     8521 cgctacggca attccgttat ccgcgccaag cgtcgtgccg gttgctttga cccattctcc
     8581 gtcaatgcgg ggtttgatcg gatcgttatc aaaatcaatt tgagtatctg gcgttttttg
     8641 gcaaaccata tccaaatgcc cttggagaac aacaaccggt ttattctcgt aaccgggagc
     8701 ggccggttta cgaatgacaa tattccctgc ggcgtcttca ctccagtccg aattgagttt
     8761 ttgtgctact cccttaatat acgctcggat tttttcttcc tttttcgaag gacgtgggac
     8821 ttgagttatt tcataaaaat aatcaaaaac gcgtttaggc tcgagatctt ttaatacatg
     8881 atcagacata tttcttctcc gttattcata taattaaccg cacggcaagt taggttattc
     8941 gactaaaaat tccaatatgg aaattcgttt tgcttatatg cattgcggta attactttaa
     9001 aaaaaaggaa ggccgccgat gaaactcaaa aaaatactga tcgttgacaa tgatcccgcg
     9061 tcattagtta atttatccgc tatggtggaa aaagccggtt ttacccctgt cggcttccag
     9121 aaaccaacct tggcgatgga gtttatcgaa aaaccggaca atcatgatat tcgtatgatc
     9181 atcgcagaga tgaatatccc cggctctgac ggttttacat tccttaaagc catacaaaaa
     9241 aaaccacaac tcaaactgat gccatttctt ttcttgtccg agctccaaga tacagccgtg
     9301 ctggtgaatg cctacgaaca aggtgctgtt gattatttca tcaaaccgat tcgcaaagaa
     9361 tcgttattta ttgccaaaat caaatccatg gtggacgcat ttgagcgcac actccaaagc
     9421 gccaacacgt tattaagcgg ccaactgagc gataaaccat tagtggaatt actggcggta
     9481 tgtgaaaacg aatccttaaa cggctactta cgaatcaaac acgccgatgg gatcggagtg
     9541 ataacgtttg tgaaaggcct accggaaaaa atggctatcg tggatgcaca gcaaaaaatc
     9601 attaaaagtg aatcggaagc ttttgaagcc atgtcggatt ggtcggaggg tgaatttttg
     9661 atacgacgtg gaaacctctg atagaggtac gtcattttcc gggcggtatc gcggaagaat
     9721 acattgatcg gaaaatcata tcggccgcgt cttgcaggat aatttttttg taaaaaacag
     9781 cgtcaatttg ttcatctacc aatccacgat atccttcgga agtaaggtgg tcgtgtaatt
     9841 ttttatttac gccacgcagt aatgcgtttc gaatacgact cttccttctt atatccatgt
     9901 ccccgcttga ttccaaatat tctctatgct taaaaaccgt ttctaaaacg gcatcaatac
     9961 cttcgttttg cgttgcaact gtttttaaaa cgaccggcat ccatggtgca tgggtgtaac
    10021 gaatttgtac ggcagcttct aattcacgtt tgaggatgtc cgcctgatcg cgatcggatt
    10081 tattgactac aaataaatcc gcgatctcca taatgccggc tttcatggcc tgcaccccat
    10141 cgcctgattc cggtacaagc acaacaacta cggtatcggt gtattggacg atatctattt
    10201 ccgattgacc tacaccgacc gtttcaaaaa taataacatc gtacccaaac gcatccaaca
    10261 agtctgccgc ggcgtgcgcc gtatgcacca gaccgcccat cgccccgcgg gtagccatac
    10321 tgcgaataaa aatattctca tgcgtagaaa tcgcttgcat tcgaaaacgg tcacccaata
    10381 tggcgccgcc cgtaaatgcg ctggtcggat cgacggcaat aataccgaca cgtttccctt
    10441 gttgtgccaa tcctaatgca gcggcattga ctaatgtact tttaccggca cccggagggc
    10501 cggtaatgcc gatacggtag gcttgtcctg tatagggaag taacgctcgc aaaatatcgg
    10561 ggccgttttc cgcgtcgttc tctaccgacg acagtaatct ggacagataa cgttttcgtt
    10621 taggatcatt atgcgctaat aaacgcacgg catctatatg ttccattgag atactcatat
    10681 gctaatttcg aatagccccg gtcaaaatac gaaaagcaat tcgcatcaga tcggcaaaaa
    10741 aataaaacgg aatagtcaac caatgatacc aacgggcata tttgatgagc aatttcatat
    10801 tactaaaaaa acgtaaccga atttttcgta atgataattg cccacccgtc gaagcggata
    10861 ttttatgcca aatttttgcc tgcggaatat acaccaattc aaagccggcc tgttttgctc
    10921 gaaagcaaaa atctacatct tccgaataca tatgatatga agtatcgagt cccggcacgc
    10981 tcttaaaaac atcgcaccga atcagcatcg cacagccggt gatgtaatcg ctcgtttttg
    11041 cttcttcgta ttgtaggttt tctttttcac gaatgccaat atgacgcgcc gtacctttgt
    11101 ataatttaaa ttcggctccg gcaaaccaaa tctgatcatt cggttcaaag tagtagattt
    11161 taggacccaa aataccagct tgcggatagt gcgccagccc ctcggataga tgaacaagac
    11221 actttttgtc catcacagta tcgttattca acaataaaac agcatcggcg ttgttgttca
    11281 gcgcgtattg aataccgaca ttattccctc ccgcaaaacc gagattagct tcattaccga
    11341 tgtatttgat ttttccggag tgaatttcag aattaaattt ttctttcaga taatcgccgc
    11401 ttccgtcagg agacgcatta tcaaccagca cgatagtaaa agatgtgaaa tcggagtcta
    11461 agcaagagcg aacgcacgct tcggtgagat gccgtccgtt atacgataag atgattacaa
    11521 aaatatgcgg cgtttgcata catactacgg gttatcgacc atcctgaaat gcaatatggg
    11581 ataacggtac tgtgacggta aacgtcgaac ctttaccttc gacgctggtt acgatgattt
    11641 ttccgccatg taattctacg attgttcgtg cgagtgataa tccgataccg gcgccctccg
    11701 ccgaacgcgt cgtggtctga tccacttgat aaaatttatc aaaaatacgc tcaacatatt
    11761 tactcggaat tcctgaaccg gtgtcatgta catgaatttc aatagcatcg gtagtcatca
    11821 gcgcatgcat acctacttta ccgccaaaac ccgtaaactt aatggcattt tcgatcaaat
    11881 gaccaatggc ttgtataaaa cgaggaccat ccatagccac gatgggaaac gtatcttcga
    11941 ctttcatttc caagttaata tttttttcgc gggctatgtc tttatattca tcaaacgcct
    12001 tgctaagtaa ttctttgaga ttattgggat ggcgctcgat tatgatggaa ccgtattcga
    12061 tctcagccaa tgaaatgaga tcattaataa gatcggccaa acgcttactt tcgtgtaaaa
    12121 taacttcgac gaaacgctgt ttcgtttcat catctacgtc tttagattct ttcatcaacg
    12181 tactgaaacc ttgaatcgaa tgaatcggcg tacgaagttc gtgcgatatg gttgatagaa
    12241 aattagattt aagatcacgc acttcctttc gcgcacgata cccccaactt acgacttcac
    12301 ggaaaagatt attggtatcc ctcaggcgtt tgctcatcac tttggctaaa cgcgtcatga
    12361 tttcatgcga cagttttatg tcttcatgca ttaatgcatg aaacgattct tttggaaaaa
    12421 acaataattc cgtttcatac atcgtcaccg caccggccga acgcacttct tcatccaata
    12481 acgccatttc accgaaataa ctgccggcgg gaagtacagc tacggcttgt tggcgcactt
    12541 cttcacccgg cacgcttttg aaaattttca cgaacccttt ggcaatgatg tacataccgt
    12601 ccggctgcgt attaccgtct tcaaaaatca ccgtattggt cggatacact ttgagcgtaa
    12661 aatgcggtaa taaacggcgt agtgcatctt ctggtatatt tttgaaaagc gcagaagacg
    12721 ctaacgttgt aagtttgtac tcatctgagg acataaggtg ttccgt
//
LOCUS       DAOQNQ010000063        12567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1766_length_12567_cov_5.281889, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000063 DAOQNQ010000000
VERSION     DAOQNQ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12567
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1766_length_12567_cov_5.281889"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PK754_05860"
     CDS             complement(<1..189)
                     /locus_tag="PK754_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09061.1"
                     /translation="MYKNHSKIQNDSLHITRDPARLSVQPYPNFGIGFGGSAYFMQLD
                     KFRQVYRDVGKPTTSPLLW"
     gene            237..971
                     /locus_tag="PK754_05865"
     CDS             237..971
                     /locus_tag="PK754_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09062.1"
                     /translation="MQKLMRFLDANKILPIKPYMEKELRKHTGRFQPEAQRHFFANIL
                     HRAPNVLYSHLTHWFETAWLREAPHASVFRNTPLPYNIWVSRSEGMATGMEELLMHAG
                     LYDDEPRARELVWIMLAQRCARGLASLYAHANMIDYDAARQFQVTWTPQGWTGDPALV
                     GFEQHLYLRMPGYGTSYVSGKYMLERLMMERSRQVGSSYSVYQYFTELYSAGMIPVSL
                     IRWQLTGLDDEVRGLMMISNEKNQGK"
     gene            complement(981..2594)
                     /locus_tag="PK754_05870"
     CDS             complement(981..2594)
                     /locus_tag="PK754_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB09063.1"
                     /translation="MSEPAATISSGERILVVDDSKVQLFAMSTMLKKEGFDVESTHEP
                     LTAKNMIEQKGYDLLLCDLIMPDMDGIGLLRVSKSKRPTMPVVIMTAFGDRESAIQAL
                     KEGAEDYIFKASGDRERDEFIIRIRRALERAKLQKKIFDYQTHLEQMVDERTQELKAT
                     QDQLVQSERLRSLGVITSGVAHDFNNILAVILGRSQLLSQKIHDTDVSKDLDMITRSA
                     LKGSATIKRMQDYTRIRKDERFESIALNSMLEEVIEMTKTRWQDESNSLGVTINLQSQ
                     FGDVPLVSGDASELKDVFINIIFNSVDAMREGGTLSIRTYTEQIENTLWVVTELSDTG
                     CGIPENIQSKVFDPFFTTKNQHGSGLGMSTAYGIVQRHRGHLSFKSKPGEGTTFYVKL
                     MAALFVQQLAPDVARAKEVGPPKPLSVLVVDDDEAVRTTLVEMLEIQDHEVHQAGSGK
                     EALRMIEERSFDIVFTDLGMPGMSGWEVSQEIKKRAPQTNIVMITGWGSQLDKEKAEQ
                     TGIQRILPKPVSFDEIVRAVTDFGLPLKS"
     gene            2677..3063
                     /locus_tag="PK754_05875"
     CDS             2677..3063
                     /locus_tag="PK754_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNB09064.1"
                     /translation="MEKQRFSSGAPWEPIVGYSRSVRAGQFIYVSGTTATDENGKLVG
                     KNDAHAQTVQTIINIEKALHKAGANLTDIVRTRIYVVNIERDWEIIGRVHGEFFKNIR
                     PATAMVEVRKLIDPDMLVEIEAEAVV"
     gene            complement(3064..4272)
                     /locus_tag="PK754_05880"
     CDS             complement(3064..4272)
                     /locus_tag="PK754_05880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNB09065.1"
                     /translation="MNEPQAVLIDAIRTPIGKIGGTLSSVRPDDMMGNLLNAMSVRHP
                     WLTHQCDDVIVGCANQAGEDGRNIARMAILSSNFPFEIPGQTINRLCSSSMQAVASAA
                     MTIQSGFARTLLAGGVESMSRAPYAVAKAEKAFPNGPMTMYDTALGWRFINPRITKRV
                     RPLSMGETAEEIFKQFAITRETQDAFAVASHRKAIAARNKGSWKDEIIALRVRQGKNE
                     VLIDTDECPREDASIETLSKLKPAFRDDGTVTAGNSCPMNDGASLLLLVSPDVAKESG
                     HREGFVLRGYAVTGIHPDIMGLGPVEATRRALQYAHVSLNDIDLIEINEAFAIQVLAC
                     MQALELDDTRVNVNGGAIALGHPLGCSGARIIGSLTNEMKRRQSRYGLATMCVGLGQG
                     MATVWERITL"
     gene            complement(4269..4538)
                     /locus_tag="PK754_05885"
     CDS             complement(4269..4538)
                     /locus_tag="PK754_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09066.1"
                     /translation="MPDFKPLHVIQTSSDGGCINADETRLIGQGWQRRYVADPRAAQE
                     AKLNYEEMGFEVQLLPIDVQSVSAECNGCRAAIEKYQIIYTRKSS"
     gene            complement(4531..5133)
                     /locus_tag="PK754_05890"
     CDS             complement(4531..5133)
                     /locus_tag="PK754_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNB09067.1"
                     /translation="MSTRNIAILTEEHRIIEYVLNALERMANKTATEHTVDVAIGRKV
                     ITFLREFADQCHHGKEEAILFKWLNARHIGMDAVAMLEDEHVAGRDLVVRMQRALDEF
                     EKNKEIGSFADDANTFIAMLRDHIHKEDHGVFAMADGHIEAADDVRLIGDYLITERDG
                     RRHQKALALAQDIGHLMNMPGFDISIIPELSKTFINSNHA"
     gene            complement(5130..5948)
                     /locus_tag="PK754_05895"
     CDS             complement(5130..5948)
                     /locus_tag="PK754_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNB09068.1"
                     /translation="MNCTLGLDVGSTYTKAIILDEDKKIRGKYMAATGSRLNEVAEEV
                     ITQACKDGGILRENIRYTMTTGYGRHLVDTRDMQVTDLTAAARGAQFLYPKTRTILDI
                     GGQTMKASRVDDRSKVLTFRLNDKCAAGTGAFIEKTARYMGYSTTDIGSLLNLSRSEV
                     GISGVCAVFAESEVISHLSEGVPPEDIMFGAILSLTDRSVQLLKRIKAESEFTLIGGI
                     LRWEKMVNSIREKLTGTVNVPEGDMPQFTAALGCALLARIRQSQTRERSASVTA"
     gene            complement(5945..6757)
                     /locus_tag="PK754_05900"
     CDS             complement(5945..6757)
                     /locus_tag="PK754_05900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNB09069.1"
                     /translation="MAYSAGVDVGSTQTKAVIINEQRQIVQRALIDTGANVTLAAQKA
                     FRQAAEAAQVSEEEIEYVIGTGYGRYKVTFGNKQITEISCHGRGAVHMFPKTRTVLDM
                     GGQDSKAIRVSDKGEIIDFCMNDKCAAGTGRFLGAAAMTLHIPLDNLGQTALEAMKAV
                     RITTTCTVFAESEILSWLGKGKKVEDILWGVHQSIAARSIGLLRRVGIEDEITFTGGV
                     ARNVGMIKALEDSLQRKINVGADSHFMGALGAALFAMDHIVAARQPQKEAVA"
     gene            complement(6781..8061)
                     /locus_tag="PK754_05905"
     CDS             complement(6781..8061)
                     /locus_tag="PK754_05905"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNB09070.1"
                     /translation="MDTLVGRGTQRGAALFREWFDELGHAAETGKPSAYVFVMGSLNE
                     ILKSFDMPVVFPEINSLQTAVRRVAPEFLAEAEDYGYSPDICGYVKADVTVQLRKGQH
                     PMGRIPQPSVAVLTNACNTYIKWAEIWERMYKIPVVTIDVPGTRGRREDPFAKGSHFE
                     NDRKYIAAQLKELITTCEQITGKKFDIDKLRETMQYANDMSVSWKNILDCNKSKPSVL
                     NALTDGTIYLGVANGFRGSKEGSVYFKELEEEMKYKTANGIGTVTDEKFRLAFVGVPC
                     YPIFRRFNDFFSDWGGTFVQSTYLYFAAGGANLGFQYDLQNPIDSLAEGVLFSVRDAM
                     DSMFHQDQVLLSMIDDYAIEGVVYHPIKSCRTVSSGLADGRKHLMAQSDVATLFLESD
                     MMDKRVVSEAQMKNRVDAFFEGLISRRQIAAAAV"
     gene            complement(8081..9238)
                     /locus_tag="PK754_05910"
     CDS             complement(8081..9238)
                     /locus_tag="PK754_05910"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HNB09071.1"
                     /translation="MIHVPLEGKTLEETLYNCRELAEDPSFPTVKAWRENGGKVLGHF
                     QVYFPEELAYAAGMLPFKVCGAQVEGMEAESHFGSYLCSVIKTSLELALTKQITLDMF
                     VTHPICDAARNLAAIWGRNFSYPCQILYLPQNPNSRHSAEYLRGEYHRLLTDIETIVG
                     HKVRDEDIANAIGVYNESRRLMRELYDIKRETPWLIAAEDAYALIAIAGCLPRDAYNA
                     LMQRVLPQIRERRLQPKDKMRIVFEGGFCEQPPLDLIQVIGQSCYIVDDDLLIGLRYI
                     LEDVPSTGDPLANLAESYIERSSYSPVQHDLRKPKEEMLLERIRHSRAEAAIITAAKM
                     CEPGLEEQVAYTKALDQAGVPYFVSEFEENQTTFDHIGIQLETFVENLIFQ"
     gene            complement(9252..9974)
                     /locus_tag="PK754_05915"
     CDS             complement(9252..9974)
                     /locus_tag="PK754_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB09072.1"
                     /translation="MAKPMIGIDLSNKVAVLTGAAGGIGSVIAQKLEKAGAKVYAVDM
                     IQPPHGLWKKTDVGSFDETGRCVEEILKEASRIDILINNAGITRDAVIWKMDESDWDD
                     VMRINLKGAFNLIHHISPAMRYQNAGRIINITSINGMRGKFGQSNYAASKAGLIALTK
                     SVARELARHQVTANAIAPGLIGTPMVEKMPPESLQQSVQEICLGHPGKPEDIAYAVLF
                     LSSEWARHITGETLRVDGGQYM"
     gene            complement(9959..10720)
                     /locus_tag="PK754_05920"
     CDS             complement(9959..10720)
                     /locus_tag="PK754_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNB09073.1"
                     /translation="MPNKIKEQITDGILHLTLCNTRGNILDGEMMGEITSAIEGATRD
                     PHIKAITFRGEGDHFSFGASVPEHQKDRVAEMLASFHKLFRTLISFSRPTIAIVRGQC
                     LGGGMELAAFCHWIFAHEKAQFGQPEIQLAVFPPVASLALPLRIGQPMSDDLNLTGRS
                     IGAEEAYRIGLVQHISPDPDTTASEFIRQHIIPKSASSLRIATTASRRIYHEQFLKHI
                     DALEAMYLQELMGTHDANEGINAFMQKRKPEWQNR"
     gene            complement(10739..11827)
                     /gene="oah"
                     /locus_tag="PK754_05925"
     CDS             complement(10739..11827)
                     /gene="oah"
                     /locus_tag="PK754_05925"
                     /EC_number="3.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase"
                     /protein_id="HNB09074.1"
                     /translation="MFKNHDLVPDYEFKEIRYELKPCKDPSGHVLPDLFSAWITLNNP
                     SQLNSYTTTMVKEVILAFRKASNSRNVIAAVFTGEGDRAFCTGGNTKEYAEYYSGQPE
                     EYKQYMRLFNDMVTSILHCDKPVICRVNGMRVAGGQEIGMACDFSIASDLAVFGQAGP
                     KHGSAPDGGSTDFLPLFVGIENAMISGTLCQLWSAHKALRLGILTDIVPVLKKDGQFI
                     PNPLVITDRYTDNMGRIVHGEFHTGAEKDKAKEIMKSCVTDFSKLDATVDQLITSLLM
                     MFPGCVSKTIESLRKHKLAHWDKNRETNRSWLGLNMMTEAKAGFRAFNEGTKESREVD
                     FIKMRQLLAAGEKWSDELIEKIHPKNKK"
     gene            complement(11840..>12567)
                     /locus_tag="PK754_05930"
     CDS             complement(11840..>12567)
                     /locus_tag="PK754_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNB09075.1"
                     /translation="FASHIVVPARFLCPVEVGGADELFGKSGVTLRELCAIADAITTP
                     FQSIKQTGLIKDDVAIFVGAGGVGGFGIQIAQSIGAHVVVIDVDDKKLEIYKNFGAEL
                     TFNPKTVEPKEMRKSIQAWVQQNKYRSYGWKIYETSGTAKGQELAFGLLTFGSVMGVI
                     GFTLDAVQLRLSNLMAFDAKAIGNWGCEPGLYPEVLQWVKEGRIQIAPFVQKFPMSEI
                     NSVFDRLHHRQIDRRPILIPDFS"
BASE COUNT         3036 a         3086 c         3101 g         3344 t
ORIGIN      
        1 ccataaaagc ggtgacgttg ttggttttcc cacatcccga tatacctgcc ggaacttatc
       61 gagttgcata aaatacgccg atccgccaaa acctattcca aaattggggt atggctggac
      121 actcagtcgg gcggggtcac gtgtgatatg gagagaatca ttttggattt tagagtggtt
      181 tttatacatc ggctttttgg tttcgccttg cggcgattgg cttttcgaag agccttgtgc
      241 aaaaattgat gcgctttctt gatgcgaata aaattttgcc tatcaaaccc tatatggaaa
      301 aagaactacg caaacacacg ggaagatttc aacccgaagc gcagcggcat ttttttgcaa
      361 atattcttca tcgcgctcct aacgttttat actcacatct cacgcattgg tttgaaacgg
      421 cgtggttacg cgaagcgccg cacgccagtg tgttccgcaa tactccgtta ccctacaata
      481 tctgggtgag ccgttccgag ggtatggcga cgggtatgga agaacttctg atgcatgctg
      541 gtttgtacga cgatgagccg cgtgcccgtg aactggtgtg gatcatgctg gcccaacgtt
      601 gtgcgcgggg cttggcttcg ctgtatgccc atgccaatat gatagactac gatgccgcac
      661 ggcaatttca ggtaacgtgg acgccgcaag gctggacggg tgatcccgca ttggtaggat
      721 ttgaacagca tctgtatctg cgcatgccgg gttatggtac aagttatgta agcggaaagt
      781 atatgttgga acgccttatg atggagcgca gtcgtcaggt cggttcatcc tatagtgtct
      841 atcagtattt tacagaactg tactccgccg gtatgatccc cgtgtcattg atacgatggc
      901 aattgacagg attagatgat gaagtgcgtg gtctgatgat gatatcaaac gaaaaaaatc
      961 aggggaaatg atttttataa ttagcttttt agaggcaaac cgaaatcggt tactgcgcga
     1021 acgatctcgt caaatgaaac aggtttaggt aggatacgct gaatgcccgt ttgttctgct
     1081 ttttctttat cgagttgaga accccagccg gtgatcataa cgatattcgt ttgtggtgcg
     1141 cgtttcttaa tttcttgcga aacttcccag ccggacatac ccggcatacc caagtcagta
     1201 aaaacaatat caaaactgcg ttcttcgatc atacgaaggg cttctttacc ggaaccggct
     1261 tggtgtactt cgtggtcttg tatctcaagc atttcaacca acgtagtgcg cacagcttcg
     1321 tcatcatcta ctacaagtac agatagaggt tttggcgggc cgacttcttt agctcgcgcg
     1381 acatccggtg caagttgctg tacaaaaaga gccgccatga gcttgacata aaatgtcgta
     1441 ccttcacccg gtttgctttt aaacgaaaga tgcccgcggt gccgctgcac gataccgtaa
     1501 gcggtgctca tacccaagcc gctgccatgc tgatttttgg tcgtaaagaa aggatcaaaa
     1561 actttggatt gaatattttc aggaatcccg cagcccgtat cgcttaattc cgtcacgacc
     1621 cacaacgtgt tttcgatctg ttcggtgtag gtacgaatcg aaagcgtacc gccttcgcgc
     1681 attgcgtcaa cggaattgaa aataatatta ataaaaacgt ctttgagttc cgaagcgtcg
     1741 cctgaaacga gcggtacatc gccgaattga ctttgcaaat tgatcgtaac acccaaactg
     1801 ttggattcat cctgccaacg tgttttggtc atttcgatca cttcttccag catgctattg
     1861 agcgctatgg attcgaagcg ttcatcttta cggatgcgcg tgtaatcctg catgcgtttg
     1921 atcgtggccg atcctttgag cgccgatcgc gtaatcatat ccagatcttt ggacacatcc
     1981 gtatcgtgga ttttctgaga aagcagttgc gaacgtccca atatgacggc gaggatatta
     2041 ttaaaatcgt gagcgacacc actggtgatg acacccaacg aacgcaaacg ttcggactga
     2101 accaactggt cctgcgtcgc tttcagttct tgcgtccgct cgtccaccat ttgttcaagg
     2161 tgagtctggt agtcaaatat ttttttctga agtttggcgc gttcgagggc acgtcggata
     2221 cggatgataa attcgtcccg ctcgcggtca ccgctggctt taaaaatata atcttccgcg
     2281 ccttctttga gcgcctggat ggctgattca cgatcgccga atgccgtcat aatcacgacc
     2341 ggcatggtgg ggcgtttgga tttggaaacg cgcagtaatc cgataccgtc catatcgggc
     2401 atgataagat cgcacagcaa aaggtcgtaa cctttttgct cgatcatgtt ttttgcggta
     2461 agcggttcgt gcgtactctc gacatcaaag ccttcttttt tgagcattgt gctcatcgca
     2521 aaaagctgaa ctttgctgtc gtcgactacc aagatacgct cgccggatga gatagtggcg
     2581 gctggttcac tcatagcgca tgtaatgtat aaaaaacata ctggaataga aaataaaaaa
     2641 attgttcttt gtctcataca agatcaagga aacactatgg aaaaacaacg cttcagttca
     2701 ggtgcaccat gggaaccgat tgtcggctat tcacgatccg ttcgcgcagg tcaattcatc
     2761 tatgtatcag gaacgaccgc gacggatgaa aacggtaagt tggtcggaaa aaatgacgcg
     2821 cacgctcaaa ctgtgcaaac gatcattaat atcgaaaaag cattgcacaa agccggcgcc
     2881 aatcttaccg atatcgtgcg aacacgtatc tatgttgtca atatcgagcg cgattgggaa
     2941 atcatcgggc gggtgcatgg cgaatttttt aaaaacattc gcccggctac agcgatggtt
     3001 gaagtgcgga aattgataga tccggatatg ttggtggaaa tcgaagcgga agcggtagtc
     3061 tgatcaaagc gtaatgcgct cccaaaccgt agccatgcct tgccccagac cgacgcacat
     3121 cgttgccaat ccgtaacgcg attgacggcg tttcatctcg ttggtaagtg aaccgatgat
     3181 gcgtgcgccc gaacagccca acgggtgccc caaagcgatc gcgccgccat tgacattgac
     3241 gcgggtatca tccaattcca aagcctgcat acaagccaaa acttggatcg caaaggcttc
     3301 attgatttcg atcaaatcta tatcgttgag agatacatga gcatattgca aagcgcgacg
     3361 tgttgcttcc accggtccta atcccataat gtcgggatga attcccgtga ccgcatatcc
     3421 gcgcagtaca aatccttcac gatgcccgct ttcctttgct acatcaggcg aaacaagcag
     3481 aagcagtgac gcgccgtcgt tcatcggaca actgtttcct gccgtgacag tgccgtcatc
     3541 acgaaatgcc ggtttaagtt tggataatgt ttctatagaa gcgtcttcgc gcggacattc
     3601 atccgtatct atgaggactt cgttttttcc ttggcgcaca cgtagcgcaa tgatctcatc
     3661 tttccacgat cccttattcc gcgcagcgat agctttgcga tgactggcta cggcaaaagc
     3721 atcttgcgtt tcacgggtga tggcaaattg tttgaaaatt tcttcggccg tttcgcccat
     3781 agacagaggg cgcacacgtt tggtaatacg cggattgata aatcgccaac ccagcgcagt
     3841 gtcgtacatg gtcatcggcc cgttggggaa agccttttcg gctttggcga cggcgtaggg
     3901 cgctcgggac atgctctcaa cgccgccggc cagtagcgtg cgggcaaatc cgctttggat
     3961 cgtcatcgcg gccgaagcaa cggcctgcat ggatgaactg cataaacggt taatcgtttg
     4021 cccgggtatt tcaaaaggga aattcgaact taaaatagcc atacgtgcga tattgcgtcc
     4081 gtcttcgcca gcctgattgg cgcagccgac gatcacgtca tcgcattggt gggtcagcca
     4141 cggatgccga acggacatag cattgagcaa atttcccatc atgtcgtcgg gacgaacaga
     4201 ggataatgtt cctccgattt ttccgatggg tgtgcggatc gcatcaataa gtacagcttg
     4261 gggttcattc atgacgattt gcgggtataa atgatttggt atttttcgat ggccgcacgg
     4321 caaccgttgc attcggcgct tacggattgt acatcgatcg gcaataactg cacttcaaaa
     4381 cccatttctt cataattgag tttagcttcc tgagccgcac gcggatcagc gacatagcgg
     4441 cgttgccaac cttggccgat cagacgggtt tcatccgcgt tgatacaccc gccgtcggaa
     4501 ctggtctgaa tcacgtgcaa tggtttgaaa tcaggcatgg ttgctattaa taaaagtctt
     4561 ggataattcg ggaataatgg aaatatcaaa acccggcata ttcatcagat ggccgatatc
     4621 ctgcgccaga gctaacgctt tttgatgacg tcgcccgtcg cgctccgtga tcaaataatc
     4681 cccaatcaat cgcacatcat ccgccgcttc gatgtgtcca tccgccatag caaaaactcc
     4741 gtgatcttct ttgtgaatat gatcgcgcaa catcgcgatg aaggtattag catcatcagc
     4801 gaaggaacca atctctttat ttttctcaaa ttcatccaaa gcgcgttgca tgcgtacgac
     4861 gagatcacgc ccggcgacgt gttcatcttc gagcattgcg acagcatcca tgccgatatg
     4921 gcgagcgttt agccatttga ataaaatagc ttcctctttg ccgtggtggc attgatctgc
     4981 aaactcgcgt aaaaacgtga tcactttgcg accgatagct acatctacgg tgtgttctgt
     5041 tgcagtttta ttggccatac gttctaaggc gttcaagacg tattcgatga tgcggtgctc
     5101 ttcggttaag atggcaatat tgcgtgtact catgccgtca ccgaagccga acgttcccgt
     5161 gtctgactct ggcgaatacg agctagcaaa gcgcatccga gtgccgccgt aaactgcggc
     5221 atatcgcctt ccggaacatt gactgtgccg gtaagctttt cgcggatact gttcaccatt
     5281 ttttcccaac gtaaaatgcc gccgatgagc gtaaattcgc tttcggcttt gatgcgcttc
     5341 aaaagttgta cggaacggtc ggtaagtgaa aggatggcgc cgaacataat gtcttccggg
     5401 ggaacgcctt ccgacaagtg gctgatcact tccgattcag caaaaacggc gcacacaccg
     5461 gagatgccga cttcggaacg agacaaattg agcaatgatc caatatctgt ggtgctataa
     5521 cccatatagc gcgcagtttt ttcgatgaat gcgcccgtac ctgccgcgca tttatcgttg
     5581 aggcgaaatg tgagcacttt ggagcgatca tccacacgac tggctttcat cgtttgcccg
     5641 ccgatatcaa gtatggtacg tgttttcgga tataagaatt gcgctccgcg tgcagcggcc
     5701 gtaagatcgg tgacttgcat atcccgcgta tctacgagat gacgaccata tcccgtagtc
     5761 attgtgtagc gaatattttc cctcagaata ccgccatcct tacaggcttg agtgatgact
     5821 tcctcggcca cttcattgag ccgggagcct gttgccgcca tatatttacc gcgaattttt
     5881 ttatcctcgt cgaggatgat agctttggtg taggtagagc ctacgtcgag tccgagtgtg
     5941 cagttcatgc gacagcctcc ttttgcggtt gacgggcggc gacgatgtga tccatcgcaa
     6001 aaagtgccgc accgagcgca cccatgaagt gggaatcggc gccgacattg attttgcgtt
     6061 gcagcgaatc ttcaagcgct ttgatcatac cgacattgcg ggcgacaccg ccggtaaaag
     6121 tgatttcatc ttcgataccg acacggcgca aaaggccgat ggatcgcgcc gcgatagatt
     6181 gatgtacacc ccagagtata tcttcgactt ttttgccttt acccaaccaa gataaaattt
     6241 ccgattccgc aaaaacggtg cacgtcgttg tgatacgaac ggccttcatc gcttcgagcg
     6301 ccgtttgtcc gaggttgtcc agcggaatgt gcaatgtcat tgctgccgcg ccgagaaaac
     6361 gtcccgtacc ggcggcgcat ttgtcattca tacaaaaatc aatgatctcg cctttatcgc
     6421 tcacacgaat tgctttgcta tcttggccgc ccatgtcaag caccgtgcgt gttttgggaa
     6481 acatgtgtac tgcaccgcgc ccatgacaac tgatttcggt gatttgttta ttgccgaatg
     6541 tcactttata acgaccgtat ccggtgccga tgacgtattc gatttcttct tcggaaacct
     6601 gagcggcttc ggctgcttgt cgaaatgctt tttgcgccgc taaagttaca ttggcgccgg
     6661 tatcgatcag tgcgcgttgc acgatctggc gttgttcgtt gatgatcacg gctttggttt
     6721 gggtggaacc tacgtccacg cctgcagaat aagccataag gattctcctt ggttattttg
     6781 tcataccgcg gccgccgcta tctggcgccg tgaaatgagt ccttcaaaaa aagcatctac
     6841 acgatttttc atttgggctt cggacacgac gcgtttgtcc atcatatctg attctaaaaa
     6901 taaagtggcc acgtcggact gagccatcag atgtttgcgc ccatcggcca atccgctgga
     6961 cacggtacgg cagcttttga tcggatggta caccacgccc tcgatcgcat aatcatcaat
     7021 catcgaaagc agcacctgat cctgatgaaa catgctgtcc atcgcatcgc gtacgctgaa
     7081 aagcacgcct tccgccaaac tatcaatcgg attttgcaga tcatattgga agccgagatt
     7141 ggcgccgccg gcggcaaaat acagataagt tgactgaaca aacgtaccgc cccaatcaga
     7201 aaagaaatcg ttaaaacgac ggaaaatcgg ataacacggc acaccgacaa aagctaagcg
     7261 aaatttttca tccgttacgg tgccgatgcc gttggcggtt ttgtatttca tttcttcttc
     7321 gagttctttg aaatacacgc tgccttcttt gctgccgcgg aacccattgg ctacgccgag
     7381 atagattgtt ccgtcagtga gcgcgttgag caccgagggt ttggatttat tgcaatcgag
     7441 aatatttttc catgacaccg acatgtcatt ggcatactgc atcgtttcgc gcagcttatc
     7501 gatgtcaaat ttttttccgg tgatctgttc gcatgtcgtg atcaattctt tgagttgcgc
     7561 cgcgatatat ttgcgatcat tttcaaagtg gctgccttta gcgaaagggt cttcgcgacg
     7621 accgcgcgtt cccggtacat caatcgtgac gaccggtatt ttgtacatgc gttcccatat
     7681 ttcagcccat ttgatatagg tattacaggc gttggtaaga acggctaccg aaggctgggg
     7741 aattcgcccc atcgggtgct ggcctttgcg cagttggacg gtgacatccg ctttgacgta
     7801 accgcagata tcgggtgaat aaccgtagtc ttcggcttcg gccaaaaact ccggtgcgac
     7861 gcggcgaacg gcggtttgta gtgaattgat ttccggaaaa accaccggca tatcaaagga
     7921 ttttaaaatt tcattgaggc tgcccatgac gaaaacatag gccgaaggtt ttcccgtctc
     7981 ggctgcgtgt ccgagttcgt caaaccactc gcgaaagaga gcggctcccc gttgcgtgcc
     8041 tctaccgact aatgtatcca tagccattcc ttccgatggg ttactggaaa ataagatttt
     8101 ctacaaaggt ctcgagttgt atgccgatgt gatcaaacgt cgtttgattc tcttcaaatt
     8161 cactgacgaa atacggtacg ccggcctgat ccagtgcttt ggtataagcc acttgttctt
     8221 ctaaacccgg ttcgcacatt ttagccgccg tgatgatggc cgcttccgcg cgggagtggc
     8281 gaatgcgttc caagagcatt tcttctttgg gtttacgaag atcgtgttgt accggagagt
     8341 aggatgatcg ttcgatatac gattccgcga gattggccaa aggatcgccc gtcgagggga
     8401 cgtcttccag gatgtaacga agtccgatca aaagatcgtc gtccacgatg taacaagact
     8461 gaccgatcac ctgaatgaga tccagcggtg gctgttcgca aaatccgcct tcaaatacga
     8521 tgcgcatttt atctttgggt tgtaaacggc gttcgcgaat ctgcggtaaa acgcgttgca
     8581 ttaaggcgtt gtaagcgtcg cgagggaggc aaccggcaat tgcgatcagc gcgtacgcat
     8641 cttctgcggc gataagccaa ggcgtttcgc gtttgatgtc gtacagttcg cgcatcagac
     8701 gacggctttc attgtaaacc ccgatcgcat tggcgatatc ttcatcacgg actttatgcc
     8761 cgacaatagt ttcgatatcc gtgagtaagc gatgatattc accgcgcaga tattccgccg
     8821 aatggcgtga attgggattt tgcggcagat ataaaatctg gcaagggtat gaaaaattac
     8881 gcccccatat agccgcaaga ttacgtgcgg catcgcagat cggatgcgtt acaaacatgt
     8941 ccagcgtgat ctgtttggtg agcgccagtt cgagcgaagt tttgataacg gagcaaaggt
     9001 acgaaccgaa atgcgattcg gcttccatgc cttcgacttg cgcaccacaa actttaaaag
     9061 gcaacatccc tgcggcatac gcaagttctt ccggaaaata tacctgaaaa tgaccgagta
     9121 cttttccgcc gttttcccgc catgctttga ccgtcgggaa tgacggatct tccgcaagtt
     9181 cgcggcagtt atagagcgtt tcttcgagcg tttttccttc aagggggacg tggatcataa
     9241 tttcctccgg attacatgta ttggcctccg tccacacgca gcgtttcgcc cgtgatgtga
     9301 cgtgcccatt cggatgataa aaaaagtacg gcgtaggcga tgtcttcagg tttccccgga
     9361 tgacctaagc aaatttcctg tacggattgc tggagcgatt caggcggcat tttttcgacc
     9421 atcggtgtgc caatgagccc gggtgctatg gcgttggctg tcacttggtg tcgcgccaat
     9481 tcacgcgcga cgcttttggt aagcgcaatc agtccggctt tacttgcggc ataattagat
     9541 tggccaaatt ttccgcgcat gccgttgatg gacgtgatat tgataatgcg tcccgcgttt
     9601 tgataacgca tcgcaggaga tatgtgatga atcaagttaa acgccccttt gaggttgatg
     9661 cgcataacgt catcccaatc cgattcgtcc attttccaga taaccgcgtc ccttgtaatg
     9721 ccggcgttat tgatcaaaat gtcaatgcga cttgcctctt tcagaatctc ttcgacgcat
     9781 cggccggttt catcaaaact tccgacatcg gtttttttcc aaagaccatg cggaggctgt
     9841 atcatatcta cggcataaac ttttgcgccg gccttctcaa gcttttgcgc aatgaccgaa
     9901 ccgatacccc ccgccgctcc ggtgagtacg gcgactttat ttgatagatc aattccgatc
     9961 atcggttttg ccattccggt tttcgttttt gcatgaacgc gttgattccc tcattggcgt
    10021 catgcgtgcc catcagttcc tgcaaataca tcgcttccaa agcatcaatg tgtttcaaaa
    10081 attgttcgtg atagatgcgt cgactggcgg tcgtagcgat acgtaaagaa gaagcgcttt
    10141 tggggataat gtgctggcga ataaattccg atgcggtcgt gtcggggtcg ggggaaatat
    10201 gttgtactag tccgatgcga tacgcttctt ctgcgccgat gcttcttccg gtaagattga
    10261 gatcgtcgga catcggttga ccgatccgca gcggtaaagc caatgaagcg accggaggaa
    10321 atacggctaa ctgaatttcc ggttgaccga attgcgcttt ttcatgagca aatatccaat
    10381 gacaaaacgc ggctaactcc attcccccgc cgagacattg gccgcgcacg atcgcaatcg
    10441 ttgggcgtga aaaagaaatc aaggtacgga aaagtttatg aaacgaagca agcatctccg
    10501 cgacgcgatc tttctgatgc tctggtacac tggcgccaaa tgaaaaatga tcgccttccc
    10561 cgcgaaatgt gatggctttg atgtgtggat cgcgggttgc tccctcgatc gccgaggtga
    10621 tttcacccat catttccccg tccaaaatgt ttccgcgcgt attgcaaagc gtcagatgga
    10681 gaatgccgtc agtgatttgt tcttttattt tattaggcat ggattgtcgt tgtatagttt
    10741 attttttgtt tttgggatga attttttcga tcaactcgtc ggaccatttt tctccggcgg
    10801 ccaaaagctg acgcattttg atgaagtcca cctcgcggct ttctttggta ccttcgttga
    10861 atgcgcggaa acccgctttg gcttcggtca tcatattgag gcccagccat gaacggttgg
    10921 tctcacggtt tttatcccaa tgcgccagtt tgtgtttacg caacgactcg atcgtcttac
    10981 ttacgcaacc cgggaacatc atgagcagtg acgtgatcag ttggtcaacc gtcgcatcca
    11041 gttttgaaaa atcggttacg caggatttca tgatctcttt ggctttatct ttttctgcgc
    11101 cggtgtgaaa ttcaccatgg acgatacgtc ccatgttgtc agtgtaacga tctgtgatca
    11161 caagaggatt gggtataaat tgtccatcct ttttcaaaac aggaacgatg tcggttagaa
    11221 tgccaaggcg cagggcttta tgagcagacc atagttgaca caaggttccg gagatcatgg
    11281 cgttttctat gccgacgaaa agcggaagaa aatcggtgga accgccgtcc ggtgcggagc
    11341 cgtgtttggg acccgcctga ccgaatacgg caagatcgct ggcgatggaa aaatcacagg
    11401 ccatgccgat ttcctgacct ccggcaactc gcataccgtt aacacggcag atcaccggtt
    11461 tgtcacagtg aaggatggat gtcaccatgt cattaaacaa acgcatatat tgtttgtact
    11521 cttcgggttg accggaataa tactcggcgt attctttggt attgccgccc gtacaaaatg
    11581 cgcgatcgcc ttcacccgta aatacggcag cgatgacatt gcgggaattg gaagctttgc
    11641 gaaaggccag gatcacttct ttgaccatcg tggtcgtgta agaattaagc tgtgacggat
    11701 tattgagtgt aatccatgca ctgaaaagat ccggcaaaac atgccccgaa ggatctttgc
    11761 aaggtttcaa ttcatatcgg atttctttga actcataatc cgggacaagg tcatgatttt
    11821 tgaacatgga atctcctgat taactaaagt cgggtataag tatcggccgc ctgtcaatct
    11881 gacgatgatg caagcgatca aatacagaat tgatttcact cattggaaat ttttgcacga
    11941 agggtgcgat ctgaatgcgt ccctctttga cccattgcag tacttcggga tacagacccg
    12001 gttcgcaacc ccagttaccg atagctttgg catcaaacgc catcagattg ctgagtcgga
    12061 gttggaccgc atccagcgta aatccgatta cccccatcac ggagccgaag gtgagtaggc
    12121 cgaacgcaag ttcctggcct ttggctgtac ctgacgtttc gtagattttc catccatagg
    12181 agcggtattt gttttgttga acccacgcct gaatactttt gcgcatttct ttaggttcga
    12241 cggtttttgg attaaatgtc agttccgcgc cgaaattttt atagatttct aattttttgt
    12301 cgtccacgtc aatgacgaca acatgtgcac ctatagattg ggcgatctga ataccaaatc
    12361 cacccacgcc gcccgcaccc acaaaaatag ccacatcatc tttgatcaaa cccgtttgtt
    12421 tgatggattg aaacggcgtc gtgatcgcat ctgcgatggc gcacaattca cgcaaggtga
    12481 ctccggattt cccaaagagt tcatccgctc cgccgacttc gaccggacat aaaaagcgcg
    12541 ccggtacgac gatgtgcgaa gcaaaac
//
LOCUS       DAOQNQ010000064        12522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1775_length_12522_cov_6.672816, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000064 DAOQNQ010000000
VERSION     DAOQNQ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12522
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1775_length_12522_cov_6.672816"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PK754_05935"
     CDS             complement(<1..466)
                     /locus_tag="PK754_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNB09076.1"
                     /translation="MQNWAGLQMPERIELDESSYTTTYGKFIIQPLERGFGVTIGNSL
                     RRVLLSSLPGAAITAIKIEGVLHELSTIPGVAEDVAEMVLNLKGVRFKLHNKKPDKVS
                     IRLKGPHKFTAADIQKASNGIDFEVLNPDHVIATLSKEANVEMEIRIGRGKGW"
     gene            complement(530..1153)
                     /gene="rpsD"
                     /locus_tag="PK754_05940"
     CDS             complement(530..1153)
                     /gene="rpsD"
                     /locus_tag="PK754_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNB09077.1"
                     /translation="MARYTDAVCKLCRREGEKLFLKGTKCLTDKCPIERRNYPPGEHG
                     DKRSKPSGYSIQLREKQKLRRIYGVLERQFRRYFEKAERAKGVTGTALLQILETRLDN
                     MVYRFGFASSRSQARQLVLHRHVEVNGKLVTAPSYQVKPGDDVSIREGSKKMALIHGS
                     LKRQKETSLNWIDVDKASLKGKFLNMPEREQIPVDVKEQLIVELYSK"
     gene            complement(1189..1560)
                     /gene="rpsK"
                     /locus_tag="PK754_05945"
     CDS             complement(1189..1560)
                     /gene="rpsK"
                     /locus_tag="PK754_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNB09078.1"
                     /translation="MPRKKKKLIESTGVAVIRATFNNTIVTLTNSHGEVISWCTSGKM
                     GFRGSKKSTPFAAQVAAETAAKTAIDMGLKKVEVRVKGPGAGRESAIRSLQAAGLEIT
                     AIRDLTPIPHNGCRPPKKRRV"
     gene            complement(1627..2010)
                     /gene="rpsM"
                     /locus_tag="PK754_05950"
     CDS             complement(1627..2010)
                     /gene="rpsM"
                     /locus_tag="PK754_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNB09079.1"
                     /translation="MARIAGIELPKDKRAFVALTYIYGIGRTTAKDILKKAGVDEMKK
                     IKDLTPDEENKIRTIINDNIKVEGALRSENALNIKRLMDIGSYRGLRHRRGLPARGQR
                     TRTNSRTRKGKRKTIGGLKKVEAKK"
     gene            complement(2036..2149)
                     /gene="rpmJ"
                     /locus_tag="PK754_05955"
     CDS             complement(2036..2149)
                     /gene="rpmJ"
                     /locus_tag="PK754_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNB09080.1"
                     /translation="MKVRSSVRKICVKCKIIRRKGVVRVICDNKKHKQRQG"
     gene            complement(2162..2380)
                     /gene="infA"
                     /locus_tag="PK754_05960"
     CDS             complement(2162..2380)
                     /gene="infA"
                     /locus_tag="PK754_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNB09081.1"
                     /translation="MAKEGLIKVDGTILETLPNATFIVKLENGHQVHAHISGKMRMNF
                     IRILPGDKVTIELSPYDLSRGRITYRYK"
     gene            complement(2475..3233)
                     /gene="map"
                     /locus_tag="PK754_05965"
     CDS             complement(2475..3233)
                     /gene="map"
                     /locus_tag="PK754_05965"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNB09082.1"
                     /translation="MIRLKSGAEIDKIRESCRIIVEAMMVAAKMVKPGLRLMDLDAAI
                     ESAIKSRGGKPAFKGYHGFPNNACLSTDDIVVHGIAKPGDILQEGQILGVDIGVLKNG
                     FYGDSAVTLPVGEISEEKKKLLQVTRESLYLGIQKAVPGNRVHDISAAVQTYVEHHGF
                     SVVRELVGHGIGRELHEDPQVPNFGKAGTGARLKEGMVFCVEPMVNAGTYEVYTSKDN
                     WTIHTKDGKPSAHFEHTVVVTSNGPEILTQSDLF"
     gene            complement(3239..4570)
                     /gene="secY"
                     /locus_tag="PK754_05970"
     CDS             complement(3239..4570)
                     /gene="secY"
                     /locus_tag="PK754_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNB09083.1"
                     /translation="MLQTFANVFKIPELRKRILFTLGVLLIERIAAHIPAPGIYAEAL
                     GQYFRQAENTLLSFYDLFAGGGLSQATIAALGIMPYISTSIIIQLLGTTVPYLQRLQR
                     EGEEGRKKINQLTRYGTVALAAVQAIGVATWLCSLSVVIGSQEVKVVPDQGFTFYLMT
                     MVTMTTCTMFIMWLGEQITERGIGNGISLIIAIGILARFPTAIIDEITQISTGNRNII
                     LELILVTILLGIVAAIIFITQGIRKIPVQYARRVIGRKVYGGQSTHIPMRINIAGVMP
                     IIFAQSIMFVPNTVATFFPGDFSEWITAHFSIDSITYNLFFLILIVIFTYFYTAIAMN
                     PVEVADNLKKQGAFIPGVRPGKKTSDFIDNILTRVTLPSAIFLGVIAILPFFVRHATD
                     VSYSFASFFGGTSLLIVVGVGLDTIQQIESHLVMRHYDGFLKSGRIRGRRG"
     gene            complement(4606..5091)
                     /gene="rplO"
                     /locus_tag="PK754_05975"
     CDS             complement(4606..5091)
                     /gene="rplO"
                     /locus_tag="PK754_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNB09084.1"
                     /translation="MNLSNLTPAKGSRRKMKRIGRGEGSGHGGTSTRGNKGQKSRSGL
                     NRLAGFEGGQMPLQRRLPKYGFTNIFRVEFQTVNLADLAELDNKRVDGATLQAAGLIK
                     KKSAPVKLLGTGEVTKAFEVEVHAISATAKEKIEKAGGKVTVLGIKKTVLEKRKKKAS
                     K"
     gene            complement(5189..5365)
                     /gene="rpmD"
                     /locus_tag="PK754_05980"
                     /pseudo
     CDS             complement(5189..5365)
                     /gene="rpmD"
                     /locus_tag="PK754_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014981721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
     gene            complement(5389..5907)
                     /gene="rpsE"
                     /locus_tag="PK754_05985"
     CDS             complement(5389..5907)
                     /gene="rpsE"
                     /locus_tag="PK754_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNB09085.1"
                     /translation="MKKKEAQDNPADVELKEERVVHINRVAKVVKGGRRFSFNAIVVV
                     GDGKGKVGIGLGKANEVPDAIAKGKEDAKKRMIFVPLHGGTLPHEVSAKFGAGKVLLK
                     PATKGTGIIAGAVIRAVVESVGITDILTKCKGSSNPHNVVKATMKALSEVTNAREVGA
                     VRGMTVMDVFTK"
     gene            complement(5957..6319)
                     /gene="rplR"
                     /locus_tag="PK754_05990"
     CDS             complement(5957..6319)
                     /gene="rplR"
                     /locus_tag="PK754_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNB09086.1"
                     /translation="MADKNIVKAVKRQRVKYRIRKRIIGTAEKPRLSVYRSLNHIYAQ
                     LINDEAGHTIAQVSSKTKGIQKESGKVSLSKAVGMSIAKLAKEKGIEVAVFDRNGYKY
                     HGRIKAVAEGAREAGLKL"
     gene            complement(6350..6892)
                     /gene="rplF"
                     /locus_tag="PK754_05995"
     CDS             complement(6350..6892)
                     /gene="rplF"
                     /locus_tag="PK754_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNB09087.1"
                     /translation="MSRIGKKPVAIPDKVTVTVKGSVVTVKGPKGELTKQFHPEAMVE
                     VKDKEIIVSRKNNTNFQRALHGSVRAIIAGMVTGVSDGYERKLDIIGVGFKAEAKGKV
                     IQFNLGFSHPILFAPPTGVEIAIPTPTSVSIKGINKELVGQVAQKIRSFKMPEPYKGK
                     GIKYSDETIRRKAGKAATKK"
     gene            complement(6927..7319)
                     /gene="rpsH"
                     /locus_tag="PK754_06000"
     CDS             complement(6927..7319)
                     /gene="rpsH"
                     /locus_tag="PK754_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNB09088.1"
                     /translation="MTDPIADYLTAIRNAIQAKKKFVDIKASNLKRDITQILLEQGYI
                     KDYANYDDGVQGIIRIFLKYDEKNRNAINQIDRASTPGLRKYAGVDKLPRVKSNLGIA
                     IMSTSKGVMTNKRAKTENVGGEVLCYVS"
     gene            complement(7374..7559)
                     /locus_tag="PK754_06005"
     CDS             complement(7374..7559)
                     /locus_tag="PK754_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNB09089.1"
                     /translation="MATKAWVAKTKKTPKFSTRTRNRCRRCGRPRAYMRKFGICRICF
                     RNLSLNGEIPGIRKASW"
     gene            complement(7594..8235)
                     /gene="rplE"
                     /locus_tag="PK754_06010"
     CDS             complement(7594..8235)
                     /gene="rplE"
                     /locus_tag="PK754_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNB09090.1"
                     /translation="MAKEAKEKKGKDKAASTGVEIPSGKRAAVGKDYKARLGEEYRKR
                     VVPALMKKFSYKNIMQVPKIEKVVINMGVGMATQEPKLLDAAVNDLLIITGQKPTIRK
                     AKKAISNFKLREGVGIACFVTLRRAAMYEFLDRLINIAVPRIRDFKGVPDKSFDGRGN
                     YTLGVKEQIIFPEIDVDKMDRVRGMDITIVTSAKTDEEAYELLKEFGMPFKKK"
     gene            complement(8269..8574)
                     /gene="rplX"
                     /locus_tag="PK754_06015"
     CDS             complement(8269..8574)
                     /gene="rplX"
                     /locus_tag="PK754_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010290402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNB09091.1"
                     /translation="MHIKKNDLVAVISGVHKGKQGKVLKVLTDTGKVIVEGINLRKKH
                     IKPNPKNPQGGIQEKENAIPASKVMLVHGGKPTRTGAKKLEDGSKVRIAKKSGEVIN"
     gene            complement(8613..8981)
                     /gene="rplN"
                     /locus_tag="PK754_06020"
     CDS             complement(8613..8981)
                     /gene="rplN"
                     /locus_tag="PK754_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNB09092.1"
                     /translation="MIQEFTRLTVADNSGAKKVMCIRVLGGTSKRYAFLGDEIIVSVK
                     DAIPGGTVKKGAVARAVVVRVRKEMRRKDGSYIRFDENAAVLINKDKEPTGTRIFGPV
                     ARELREKQFMKIVSLAPEVI"
     gene            complement(9014..9295)
                     /gene="rpsQ"
                     /locus_tag="PK754_06025"
     CDS             complement(9014..9295)
                     /gene="rpsQ"
                     /locus_tag="PK754_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNB09093.1"
                     /translation="MSEQAENKTTERNRRKVRIGTVVSDKMSKSIVVAIERKVLHKTY
                     KKYIKKTAKLMAHDEQEQAGIGDIVKVMETRPLSKNKRWRLVEVVKKAD"
     gene            complement(9342..9731)
                     /gene="rpmC"
                     /locus_tag="PK754_06030"
     CDS             complement(9342..9731)
                     /gene="rpmC"
                     /locus_tag="PK754_06030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNB09094.1"
                     /translation="MKAHEVRGLPAEELKGMINEAETNLVKMRFTNAVGQLEKKTDLR
                     STKKEIAIVKTVMREETLKTELAEAAKILEAVVSKHQIKGIPTLKGEKLNTDKAALRK
                     AVRLLESNPKRSLFVKEYKALKEIAHG"
     gene            complement(9756..10175)
                     /gene="rplP"
                     /locus_tag="PK754_06035"
     CDS             complement(9756..10175)
                     /gene="rplP"
                     /locus_tag="PK754_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNB09095.1"
                     /translation="MLQPKKVKFRRHHRGKMRGKATRGADVSFGTYGLKAMECGWITS
                     RQIEAARIAMTRYIKRGGKIWIRIFPDKPVTKKPLEVRMGSGKGAPEFWVSVIKPGRV
                     MFEIDGVTEELAKEAFRLAAFKLPLKSKFVTRGQVHA"
     gene            complement(10214..10867)
                     /gene="rpsC"
                     /locus_tag="PK754_06040"
     CDS             complement(10214..10867)
                     /gene="rpsC"
                     /locus_tag="PK754_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNB09096.1"
                     /translation="MGQKTNPTGFRLGVIKTWNSSWFDEKNFADKLQEDLTLRNYLRK
                     RLPKDARVTKIAIDRIKKETEVITITIHTARPGIVIGRKGKDVDQLREELKKITGKEI
                     RLNISEIKVPEVDSYLVAADIARQIESQIPYRRAMKNATRAAMRMGAEGIRVAMSGRL
                     GGAEMARADRYAEGRVPLHTLRADIDFAYGTAQTTYGQIGVKVWICKGEVLDTMETK"
     gene            complement(10906..11247)
                     /gene="rplV"
                     /locus_tag="PK754_06045"
     CDS             complement(10906..11247)
                     /gene="rplV"
                     /locus_tag="PK754_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNB09097.1"
                     /translation="MMEAKAITKYLRISPRKVRRVAELVRGKNTEEAISILHFTRKNA
                     SEHIEKTVRSAVANIMQAKPGADTHSLFVKEIFVDGGPMLKRGRAGSMGRRSMIRRRT
                     AHITVVVAEKA"
     gene            complement(11275..11568)
                     /gene="rpsS"
                     /locus_tag="PK754_06050"
     CDS             complement(11275..11568)
                     /gene="rpsS"
                     /locus_tag="PK754_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNB09098.1"
                     /translation="MSRSIKKGPFLDENLIGKVEVMNKNKDRKVIKTWARACTIAPDF
                     VGHTFAVHNGKNFIPVYVTENMVGHKLGEFAPTRTFRGHSGSKSAAAATAPKK"
     gene            complement(11622..12449)
                     /gene="rplB"
                     /locus_tag="PK754_06055"
     CDS             complement(11622..12449)
                     /gene="rplB"
                     /locus_tag="PK754_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNB09099.1"
                     /translation="MAVKNFRPYTPSRRYITVADFAEITKTEPEKSLVEPKKRVGGRN
                     NIGHTTSRFRGGGHKRMYRIIDFKRHRTGVEAKVVSIEYDPNRSANIALIQYVDGQKA
                     YILAPKGLQVGEKVMSGAEAEIKLGNAMPLKGIPVGTDVHNVELKLGKGGQMARSAGA
                     FANVVGREEGYVQLRMPSGEIRRVNENCFATIGQVGNLEHENVTLGKAGRKRWLGRRP
                     HNRGVVMNPVDHPHGGGEGKSPQGNPHPVTPWGQPTKGYKTRKKNHPTNRYIIKRRK"
BASE COUNT         2883 a         3144 c         2826 g         3669 t
ORIGIN      
        1 cccaaccttt gccgcgaccg atacggattt ccatttcgac atttgcttct ttgctgagcg
       61 tcgcaatgac gtgatccgga ttgagtactt caaaatcaat gccgttggaa gctttttgga
      121 tatccgccgc cgtaaatttg tgcggtcctt tgaggcgaat cgaaacttta tccggctttt
      181 tattgtgcaa tttaaagcgc acacccttga gattaagcac catttccgca acgtcttccg
      241 caacgccggg aattgtggag agttcgtgaa gcacgccttc gattttgatg gcggtgatcg
      301 cggcacccgg gagcgacgac aagagtacac ggcgaagcga attgccgatc gtcacgccga
      361 atccgcgttc gagaggctga ataataaact taccgtatgt agtcgtgtag gacgactcgt
      421 cgagttcgat gcgctccggc atctgaagtc ctgcccagtt ctgcactata gaatctcctc
      481 atttagagat tagacgtttt atataaaaag cgtcgtattc gttattgaat tacttcgagt
      541 acaactcaac aatgagctgt tctttgacat ccacagggat ctgttcgcgt tcaggcatat
      601 tcagaaattt gcccttgagc gaagctttat ccacgtcaat ccagttcagg gatgtttctt
      661 tttgacgttt cagtgatccg tggatcaacg ccattttttt gctgccctcg cgaatagaca
      721 catcgtcgcc gggtttgacc tgatacgaag gcgccgttac gagtttgccg ttaacttcga
      781 catgacggtg cagtacgagc tgacgcgctt gcgaacgcga agaagcaaag ccgaagcgat
      841 ataccatgtt atcaagacgt gtttcaagga tttgaagcaa cgccgtaccg gttacacctt
      901 tagcacgttc tgctttttca aagtagcgac ggaattgacg ttccaatacg ccgtaaatac
      961 gacgcagttt ttgtttttca cgcagctgaa tgctgtaacc cgagggtttt gaacgtttgt
     1021 cgccgtgctc accggggggg tagttgcggc gttcgatcgg gcatttgtcc gtcaagcatt
     1081 ttgtgccttt gagaaaaagc ttttcgcctt cgcggcggca cagtttgcaa acggcatccg
     1141 tatatctggc cattaagtat ctcctaaaat tgtatcttca gtcgtgtttt atacgcggcg
     1201 tttcttcggc ggacggcatc cattgtgcgg gataggcgtc aagtcgcgga tcgccgtaat
     1261 ctccaaacct gccgcttgca acgaacgaat cgctgactca cgaccggcac cggggccttt
     1321 gacgcgcact tcgacttttt tcaatcccat atcaattgcc gttttagcgg ccgtttcagc
     1381 ggcaacctgt gcagcgaaag gcgtgctctt tttggaaccg cgaaaaccca tctttccgga
     1441 tgtacaccac gaaatcactt caccgtggct gttggtcaaa gttacgatcg tgttgttaaa
     1501 cgttgcacga atcacggcaa cgcccgtgga ttcgatcagt ttcttctttt tacgcggcaa
     1561 tgcggcagcc tgttgctgct gcggattttg cttggccaaa gttctctcct tggatcaaaa
     1621 attcaattac ttctttgctt cgactttctt gagaccgccg atcgtcttac gtttaccttt
     1681 acgcgtacgg gaattggtac gtgtacgttg accgcgcgcg ggcagaccgc gacggtgacg
     1741 cagaccgcgg taggaaccga tgtccatcaa acgtttgatg ttgagcgcat tttccgaacg
     1801 cagtgcgcct tcaactttga tattatcatt gatgatcgta cggatcttgt tttcttcatc
     1861 cggcgtaaga tctttgatct ttttcatctc atccacaccg gcttttttca aaatatcttt
     1921 tgccgtagtg cggccgatgc cgtagatgta cgtcaacgca acaaatgcgc gtttgtcttt
     1981 gggtaattct atacctgcta tacgggccaa agcggttctc cctaaaattt aaatattaac
     2041 cttgacgctg tttgtgtttt ttattatcgc aaatcacgcg aacaacacct ttgcgacgga
     2101 tgatcttaca ctttacacaa attttacgaa cggacgatct gactttcata tcgtaactcc
     2161 attatttata gcgatacgtg atgcgcccgc gggataagtc gtacggtgat aattcaatcg
     2221 ttaccttatc gccgggcaga atccgaataa aattcatacg catttttcca ctgatgtgcg
     2281 catgaacctg gtgaccattt tcaagtttta caataaacgt ggcattaggc aaagtctcaa
     2341 gtatcgtccc gtcaactttg atcaatcctt cttttgccaa gttttaactc cgttctaacg
     2401 taagttattg taaaaacaat cccacaaatg ggctaccaat atataaaaaa aaattaaaaa
     2461 atcaatagct aattctaaaa taaatcggac tgtgttaaaa tttccggtcc gtttgatgtt
     2521 actactaccg tatgttcaaa atgcgctgac ggtttcccgt ctttcgtatg gatagtccaa
     2581 ttatcttttg atgtatacac ttcatatgtt cctgcattga ccatcggctc aacacaaaat
     2641 accatacctt ctttgagtcg cgctccggta ccggctttgc cgaaatttgg aacctgagga
     2701 tcttcgtgta attcgcgtcc tatcccgtga ccgactaatt cgcgtacaac tgaaaaacca
     2761 tggtgttcaa cgtatgtttg aacggcggct gaaatatcat gaactcgatt gccgggaacc
     2821 gctttttgaa tacccaaata caaagattct cgcgtcactt gaagtagctt tttcttttct
     2881 tcggagattt cgccgaccgg caatgttacg gcggagtcac cgtaaaaccc gtttttgaga
     2941 acaccaatat ccacaccgag tatctgacct tcctgcagta tgtccccggg tttggctata
     3001 ccgtgaacaa caatatcatc ggtcgaaagg caagcattat tgggaaaacc atgataacct
     3061 ttgaacgccg gtttcccgcc gcgcgatttg atagccgatt cgatagccgc atccaaatcc
     3121 atcaaacgca atccgggctt caccatcttg gccgctacca tcatagcttc tacgataata
     3181 cggcagcttt cgcggatttt atcaatctcc gcacccgact tgagccggat cattcggatt
     3241 atccccgacg gccgcggata cgaccggact taaggaaacc gtcatagtgg cgcatgacga
     3301 gatgcgattc gatttgctga atcgtatcca atccgacacc caccacgatc aaaagcgacg
     3361 tgccgccaaa aaacgacgcg aagctatagc tcacatcggt tgcatggcga acgaaaaacg
     3421 gcaatatcgc aatgacaccg aggaaaatgg cgctcggaag ggtgacgcgc gtcaggatat
     3481 tatcaataaa atccgacgtt tttttgccgg gacgaacacc cggtataaac gcaccttgct
     3541 tcttgaggtt atccgccact tcgacaggat tcatcgcaat cgcagtgtag aaatacgtga
     3601 agatgacaat taaaatgaga aagaacaaat tgtacgtaat cgaatcaatc gaaaaatgtg
     3661 ctgtgatcca ctccgaaaaa tcacccggga aaaaagtggc taccgtattc ggaacaaaca
     3721 tgatggactg cgcaaagatg ataggcataa caccggcaat attgatacgc atcgggatat
     3781 gtgtggattg accgccatat accttacgac cgatcacgcg gcgtgcatat tgtaccggga
     3841 ttttacggat accttgcgtg ataaaaataa tcgccgcaac aataccgagt aaaattgtta
     3901 ccaaaatcaa ttcaagaata atattgcgat tgcctgtcga aatttgagtg atttcgtcaa
     3961 taatcgccgt cgggaatcgc gccaaaatac cgatcgcaat aatcaatgaa ataccgtttc
     4021 cgatgccgcg ttcggtgatc tgctcgccga gccacatgat aaacatcgta cacgtagtca
     4081 tggtgaccat cgtcatcaga taaaaagtga agccttgatc cggcactact ttgacttcct
     4141 gactgccgat cactacactg agactgcaaa gccaggttgc aacgccgatc gcctgaacgg
     4201 cggcaagcgc taccgtaccg tagcgtgtaa gctgattgat ttttttgcgg ccttcttcgc
     4261 cttcacgttg taaacgctga aggtaaggca ctgtcgtacc gagaagttgg atgataatgg
     4321 atgtactgat gtacggcatg atgccaagcg ctgcgatagt cgcttgagac aatccgccgc
     4381 cggcaaataa atcataaaaa ctcaacagcg tattttccgc ctgacggaaa tactgtccga
     4441 gcgcttccgc ataaataccc ggagcgggaa tgtgcgccgc aatacgctca atcagcaaaa
     4501 cgccgagcgt aaagaggata cgtttccgca attccggaat tttaaataca ttcgcaaatg
     4561 tttgtagcat tgtgaaattc tcttgggctt atgtaagagt aaccgctatt tcgacgcttt
     4621 tttcttgcgt ttttccaaca ctgttttttt gatgccaagc accgtcacct tgccgccggc
     4681 tttttcgatt ttctcttttg ccgttgcact gatcgcatgc acttcgactt caaacgcttt
     4741 ggtcacttca ccggttccta aaagttttac cggagcgctt ttctttttta tcaagccagc
     4801 cgcttgcagc gttgcgccgt ccacgcgttt gttatccaat tcggcaagat cggccaaatt
     4861 aacagtctga aattcgacac ggaaaatatt ggtaaaacca tatttaggga gacgacgttg
     4921 caaaggcatc tgtccgcctt caaaaccggc aaggcggtta agaccggaac gcgacttttg
     4981 ccctttgtta ccgcgtgtgg atgtgccgcc gtggccggag ccttcaccac gaccgatacg
     5041 tttcatttta cggcgggagc ctttggcggg cgttaaatta ctgagattca tcgcaatcct
     5101 ctttgactaa aattgtttac gcttttttcg ctttggtaac tttataaccg gtcttttttt
     5161 cttttttcac agcttccggt tttttatcaa cggcttcaca ggaaaccata tacttcacaa
     5221 gattaaccat accgcgaacc tgcggtgtgt cgttgtgata aacggtttgg ccgattttac
     5281 gtagtccgag cgtgcgcacg gtatcgcggt gtgcgaaaat tgcgccgttg gtactgcgaa
     5341 cctgggtaat cttcaaaaat ttcatgatcg ctctttgcct ggttatcgtt acttggtaaa
     5401 tacatccatg acagtcatgc cgcggacggc accgacttcg cgcgcattgg tcacttcact
     5461 aagcgctttc atcgtggctt tgaccacatt gtgcggattg gaagaacctt tgcatttggt
     5521 aagaatgtcc gtgatgccga cggattcaac tacggcacgt atcacggcgc cggctataat
     5581 accggtacct ttagttgccg gtttgagcaa cactttgccg gcgccaaact tggccgacac
     5641 ttcgtgcgga agcgtaccac cgtgcaacgg tacaaatatc atacgttttt ttgcgtcttc
     5701 tttgcctttg gctatagcat ccggcacttc gttagcttta cctaatccaa taccgacttt
     5761 gcctttaccg tcgccgacca ccacgatagc gttaaaactg aaacggcgtc cgcctttcac
     5821 tactttcgct acacggttga tatgtacgac gcgttcttct ttgagttcga cgtcggccgg
     5881 attatcctgg gcttcttttt tcttgatttg tttcctatta ttcatcacgc ctcaccatct
     5941 atagatgtgt tttttattac aatttcaatc cggcttcacg agcgccttcg gcaaccgctt
     6001 tgatgcggcc atgatatttg tatccattac gatcaaatac ggcgacttca ataccttttt
     6061 ctttggccaa tttggcgata gacataccga ccgctttgct caacgaaact ttaccgctct
     6121 ctttttgtat gcctttggtc ttcgacgaaa cctgtgcaat cgtatgaccg gcttcatcgt
     6181 tgatgagctg agcgtaaatg tgattgaggc tgcgatacac cgacaaccgg ggtttttccg
     6241 ccgtgccgat gatgcgttta cgaatacggt atttgacacg ttgtcttttt accgctttga
     6301 ctatgttttt gtccgccatg gtgcttttaa caatttatag ttttaaaaat tacttcttgg
     6361 tcgcggcctt accggcttta cgacggatgg tttcatccga atatttgata cctttaccct
     6421 tatacggttc cggcatcttg aaagaacgaa ttttctgagc cacttgacca accaattctt
     6481 tattgatacc tttgatgctt acgctggtcg gcgtaggaat cgcaatttca acacctgtcg
     6541 gaggagcgaa aagaatcggg tgcgaaaaac cgagattaaa ttggattact ttgcctttcg
     6601 cttcggcttt aaaaccgacg ccgataatat ccaatttacg ttcatatcca tcggacacgc
     6661 ccgtcaccat gccggcaatg atcgcgcgaa cgctgccgtg caacgcacgc tgaaaattgg
     6721 tgttgttttt acgcgaaacg atgatttctt tgtctttgac ttcgaccatc gcttcagggt
     6781 gaaactgttt ggtcagttcg cccttggggc ctttaacggt gacgactgaa cctttgacgg
     6841 tcactgtgac cttatcggga atcgctaccg gtttttttcc aatacgtgac actgcttata
     6901 ctcctgcgaa taaaatttcg ttcagattac gatacgtaac acaatacttc gccgccgaca
     6961 ttttccgttt tggcgcgttt gttggtcatc acacctttgg atgtggacat gatggcaata
     7021 ccgagattgc tttttacgcg cggcagctta tctacacccg catacttccg gaggcccggc
     7081 gtgctggcac ggtcaatctg attgatcgca ttgcggtttt tctcatcgta tttcaagaaa
     7141 atacgaatga tgccttgtac gccgtcatca tagttagcgt agtctttgat atatccctgt
     7201 tcaagcagga tttgcgtaat atcgcgcttc aggttagaag ctttgatatc cacgaatttc
     7261 tttttggcct gtatcgcgtt gcgaatcgcc gtcaaataat ccgcgatcgg atcggtcatt
     7321 gacatcggat cgtctccttt gataaaaaat aaaaactcaa aaattctcta ctattaccac
     7381 gaagctttac gaatgcccgg aatctcacca ttgagcgaaa gattacggaa gcatatacgg
     7441 caaatgccga acttgcgcat ataagcgcgc ggacggccgc agcggcggca acgattgcgc
     7501 gtgcgtgtgg aaaatttcgg tgttttcttc gtcttagcaa cccatgcttt cgtagccaag
     7561 gttcaaactc ctgttagtat cgttcgtgtt atattatttc tttttgaacg gcataccgaa
     7621 ctctttgagc agttcgtatg cttcttcgtc cgttttagct gacgtcacga tggtgatgtc
     7681 cataccgcgt acgcggtcca ttttatcaac atcgatttcc ggaaaaataa tctgctcttt
     7741 gacgcctaac gtatagttac cgcgaccgtc aaacgattta tcgggaacgc ctttgaaatc
     7801 tcgaatacgc ggtacggcga tattgatcaa acgatcaaga aattcgtaca tcgcggcgcg
     7861 acgcaaggtg acaaagcaag cgatacctac gccttcgcgt aatttgaaat tggaaatagc
     7921 ttttttcgct ttgcggatag tgggtttctg acccgtgatg atcaaaagat cattgaccgc
     7981 cgcatccaaa agtttcggct cttgtgtcgc cataccgacg cccatattga tcacgacttt
     8041 ttcgatctta gggacttgca tgatattctt atagctgaat tttttcatca gcgcaggaac
     8101 cacacgttta cggtattctt caccgaggcg ggctttgtag tctttaccta cggccgcacg
     8161 ttttccggaa ggtatttcta caccggttga tgccgcttta tctttgcctt ttttctcttt
     8221 cgcttccttc gccatggctc taacgttcct ctaaaattat aatacgtctt aattgatcac
     8281 ttcgccggat tttttggcaa tgcgcacttt ggatccgtct tccagttttt tcgcacctgt
     8341 acgcgtcggc ttgccgccat gtacaagcat gactttgctt gccggaatcg cattttcttt
     8401 ttcctgaatg ccgccttgcg gatttttcgg attcggctta atgtgtttct tgcgtaaatt
     8461 aataccttcg acgatcacct taccggtatc cgtgaggacc ttcaggactt tgccctgttt
     8521 gcctttgtgc acgccggaga tcacggcgac aagatcattc tttttgatat gcatgatgta
     8581 aaacctttga tgtttcgctt attcgttcgc gcttagatta cttccggcgc gagggataca
     8641 atcttcatga attgtttttc acgaagttcg cgtgcgaccg gtccgaaaat acgggttccg
     8701 gtgggttctt tatctttatt gatcagcacg gcggcgttct cgtcaaagcg gatgtatgaa
     8761 ccgtctttgc gacgcatttc cttgcgtaca cgaactacta ccgcacgagc gacagcgcct
     8821 tttttgactg tgccgccagg aatcgcatct ttcacagaca cgatgatttc atcgccgagg
     8881 aaagcatagc gtttggaggt accgcccaat acgcggatac acatcacttt ttttgcgccg
     8941 gagttatcgg caaccgtcaa tcgggtaaac tcttgaatca tgacttatct cgccagttaa
     9001 taaatgacct gaattaatcc gcttttttca caacttccac caagcgccaa cgtttatttt
     9061 tgctcagcgg gcgggtttcc attactttta cgatatcgcc gataccggct tgctcttgtt
     9121 cgtcatgcgc catcagctta gccgtctttt tgatatactt tttgtacgtc ttgtgcaata
     9181 ctttgcgctc aattgcgaca acgatgcttt tgctcatctt atcgctgacg acggtaccga
     9241 tacgcacctt gcggcgattt ctttccgtgg tcttattttc cgcttgttcg gacatggaat
     9301 gcttctccaa tacgatttat acttatatct aaagtttttc cttaaccgtg cgcgatctct
     9361 ttgagcgctt tgtattcctt gacaaaaagg gaacgtttgg gattggactc caaaagacgt
     9421 accgctttac gcaaagccgc tttgtcggta ttgagttttt cacccttgag cgtcggaata
     9481 cctttgatct gatgtttact taccacggct tccagtattt tcgccgcttc cgccaattcg
     9541 gttttcaacg tttcttcccg catgacggtt ttaacgatgg caatttcttt tttggtcgaa
     9601 cgcagatccg ttttcttttc cagttggcct accgcattgg taaagcgcat cttgaccaga
     9661 ttggtttccg cttcgttgat catgcccttt aattcttcgg cgggcaatcc tcgtacttca
     9721 tgtgctttca tgatatctgt ctcttccggt tataattacg cgtgaacttg accacgggtg
     9781 acgaatttcg acttgagcgg aagtttgaat gccgccaaac gaaatgcttc ttttgcaagc
     9841 tcttcagtca caccgtcgat ttcgaacatg acacggccgg gtttgatcac cgagacccaa
     9901 aactcaggcg cgcctttacc ggaacccata cgtacttcta aaggtttttt cgtaaccggt
     9961 ttatcgggaa atatacgaat ccaaatttta ccgccgcgtt tgatatagcg tgtcatcgcg
    10021 atacgcgccg cttcgatctg acggcttgtg atccatccgc attccatagc tttgaggccg
    10081 taggtaccaa agctcacatc cgctccgcgg gtagctttgc cgcgcatttt gccgcggtga
    10141 tgtcttctaa atttaacctt cttaggttgt aacatgtatc agttctcctg gaacaaaatt
    10201 tctctgattg agcctacttg gtttccatcg tgtccagcac ttcgcctttg cagatccaca
    10261 ctttgacgcc gatctggccg taggtggtct gagctgtgcc atatgcaaaa tcgatatctg
    10321 cacgtaaggt atgcaacggt acgcggcctt cggcatagcg gtcggcgcgt gccatttcgg
    10381 cgccgccaag gcgaccggac atggccacac ggataccttc agcgcccata cgcatcgcgg
    10441 cgcgtgtcgc atttttcata gcgcggcgat agggaatctg gctttcgatc tgacgtgcaa
    10501 tgtcagccgc gacaaggtac gaatccactt cgggaacttt gatttcggaa atattcaaac
    10561 gaatttcttt accggtgatt ttcttaagct cttcgcgaag ttgatcgaca tctttgcctt
    10621 tacgaccgat gacaataccc gggcgtgcgg tgtggatcgt gatcgtgatg acttcggttt
    10681 cctttttgat gcggtcaatg gcgattttcg tcacgcgcgc atctttggga agacgtttgc
    10741 gcagataatt acgcaacgtg agatcttcct gaagtttgtc agcaaagttt ttttcatcaa
    10801 accagctcga gttccaagtt ttgatgactc ccaaccggaa accggtcgga ttcgttttct
    10861 gtcccaaagt attcctccgt taaacttgta tcgttaaacg atcaattacg ctttttccgc
    10921 gacgaccaca gtgatgtggg ctgtacggcg gcggatcatc gagcggcgtc ccatcgaacc
    10981 cgcacggccg cgtttgagca tcggcccgcc atctacaaaa atttctttga caaataagct
    11041 gtgcgtatca gcacccggtt tggcctgcat gatattggcc acagcggaac gcacggtttt
    11101 ttcaatatgt tctgaggcat ttttacgggt gaagtgtaaa atgctgatcg cttcttccgt
    11161 attttttcca cgcacaagtt ccgcaacgcg gcgcacttta cgcggcgaga ttctcaaata
    11221 tttcgttatt gctttcgctt ccatgatcag gctccagttc taaaatttgc gcgtttactt
    11281 tttgggtgca gtggcggccg cggcggattt actgcccgag tgaccgcgaa atgtacgcgt
    11341 cggtgcgaat tcgccaagtt tgtggccgac catgttttcc gtcacgtaaa ccgggatgaa
    11401 gttttttccg ttatgtacgg cgaaggtatg gccgacaaag tccggcgcga tcgtgcaggc
    11461 gcgggcccag gtcttgatca ctttgcgatc tttgttttta ttcataactt ccactttgcc
    11521 gatgagattt tcatcgagaa agggaccttt tttgatcgaa cgactcatgt cttatattcc
    11581 ttaattcaaa aaattcttcg ttgttgcgtc atcccgccgt attacttgcg gcgtttgatg
    11641 atataccggt tcgtcgggtg atttttctta cgcgtcttat aacccttcgt cggttggccc
    11701 cacggcgtta ccgggtgcgg attaccctgc gggctcttac cttcaccacc accatgcggg
    11761 tgatccacgg gattcataac gacgccgcgg ttgtgaggac ggcgaccgag ccaacgttta
    11821 cgaccggctt taccgagcgt gacattctcg tgttcgagat tgccgacctg accgatggta
    11881 gcaaaacaat tttcattaac gcggcgtatt tcgcccgagg gcatacgcag ttggacatat
    11941 ccttcttcac gaccgacgac attggcgaac gcaccggcag aacgcgccat ctggccgcct
    12001 ttgccgagct tcaattcgac attgtgtacg tccgtaccca cgggaattcc cttgaggggc
    12061 atggcgttac cgagtttgat ttcggcttcg gcgccggaca ttactttttc accgacttgc
    12121 aaacctttgg gtgcaagaat atacgccttc tgtccgtcca catactggat caacgcgata
    12181 ttggcgctac ggttcggatc gtattctatc gaaacaactt tggcttcgac gcccgtacga
    12241 tgacgcttga agtcaatgat acggtacata cgtttatggc cgccaccacg aaaacgcgat
    12301 gtcgtgtgac cgatattgtt acgaccgcca acccgttttt tgggttcgac gagcgatttt
    12361 tccggttccg ttttggtgat ctccgcaaag tccgcaaccg taatataacg gcggctcggc
    12421 gtgtacggtc tgaaattttt tactgccatg ttcttctctc aacttatgcc agataggttt
    12481 tcctggcgtt tcttaaactt ttcgttgtaa ttacgcgcct tc
//
LOCUS       DAOQNQ010000065        12247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1832_length_12247_cov_5.343586, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000065 DAOQNQ010000000
VERSION     DAOQNQ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12247
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1832_length_12247_cov_5.343586"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1536
                     /locus_tag="PK754_06060"
     CDS             <1..>1536
                     /locus_tag="PK754_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNB09100.1"
                     /translation="GASGIRIRIGSKKITNMGDEADLVVALNEQVLYGRIDQKAFRPG
                     TKILLDNKWAKHDVKKIQDEYTEGLNDLKSRGYDVIEIPIEQESMKHIADPRKGKNMW
                     VFGMLCCIYQRDTAKAENQIRHTFRKKKQDVVDVNLKLFRAGYAWAEQNLKFQYDIPA
                     MATDEQFVVMNGNEAISLGTLAAGIELCSMYPITPATSASHHLAECFEAAGGLIHQAE
                     DEIAAIGFAIGASYAGKTAITITSGPGMALKTEFIGLAVMAEVPLVIVNVQRGGPSTG
                     LPTKVEQGDLLYALYGQPGDAPKIVLAAATIEECFHFVILARRLAEAFRMPVILLSDA
                     NLATGVQPYPRPQINQEWFAPPIDQSPWREGVPAFDWDEETGLSRRPIPGQKNGMYTL
                     TGLAHDQRSKVAYEAATNQVGCDMRSRKLATFLQSLKPPVVNGDPEGDLLIVGWGSTL
                     GAIEEAVNHARELGGRVSSLHLRFLSPIEPGLKDIFKRFKKVMTVEINYSDNLNDPLI
                     TPE"
     assembly_gap    1537..1636
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1661..2614
                     /locus_tag="PK754_06065"
     CDS             1661..2614
                     /locus_tag="PK754_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNB09101.1"
                     /translation="MFGLKSDCVLDIPEDYYTLADYESATARWCPGCGDHGILTAVQR
                     LCSEKQLPPEKTVFVSGIGCSSRLPHYMKTYGFHGLHGRALPVANGIKFRRPDLHVFV
                     ATGDGDCCSIGTAHWIHAIRYNTNITVLLLDNEIYGLTKGQASPTSNKGTVSNTSPRG
                     SQLPPINPIQTTLGVSNVSFVAQTVDWNPAHLYATIEAGYNHPGLSFIRVLQRCPVFQ
                     KDLFQTTMSSPDAITLLTHKNGIQVDPAITKIYKNVVEHDPSDINQARTLAGNHDREV
                     IGLLYQNKNAERYDDYGAHNLGISPEDKMRAIHKELDKFAV"
     gene            2642..5152
                     /locus_tag="PK754_06070"
     CDS             2642..5152
                     /locus_tag="PK754_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNB09102.1"
                     /translation="MTSPDPMFAHLDHVLRNTSTHGTQDHTRAEQKSEKAAKPFIPES
                     KAADLSQTSLFWKNLRAFYRGDASGAIAKNRLPALLLPFRDTDRIRFDYPVWIDVSSD
                     DEKPVRTLNQLLEDAIVSFAPGADDAKILKDNRLRLEQMMRQYLSENGGSAACASIWE
                     TAVTRLTEQLHLKGETEKSFLSDTKRLKDHLPKDGHLYDFSKAMALTLLSSALRHRVA
                     DARTRLLKDAAVLRQRLQEMILIEDHRNPEAKNSSAAPGNHGYGSAFVDFTAVSRVLP
                     ASPTLPMAPARRKRITEALNILSQPDNIFKESALLIVTPDIEARYGMAWTDTMTSARI
                     SKAPSAEALRLAEAQFDAYMQTITSWVRAKRIAQLESQDRYEADVHDVYFEKFDWRAL
                     SDEEVALCAPVVVLASDEEILRDHLHDFSTALASRRPIKFFVMQSRYHSDQAAPTGVI
                     SFAHDLAAMAVAHRQAYVYQAAMVHPEQVFHGIRTGLSQSAPSLFYMLAPVSEHALLA
                     SGSALESRAFPEYSYDPQRGVQWGSRFSVEANPQADSDWPVYELTATQYDKTETLLRL
                     PFTFADHLVFDDTMTHHFMDVPPSHWTPDLVLLADYLALPPSEAYTKIPYVWTADKDG
                     VLHKTAVDHALVLMAQDRLDAWHFIQEFGGINSYHVARAIAKTRTEMTAEKERALDAL
                     HVLHQSEIEKVREETARSAMEKLSAVLLDLDTLPVHNTPSSVPSLSVSKAETSATVSD
                     TPTVSAPVEEEPVISNEAWLETIRCTSCNECINLNPNIFKYNSAKQAYIGDARAGSYL
                     HIVQAAEKCPAKCIHPGLPLNPSEDGLDALIQRAAVFN"
     gene            5182..6054
                     /locus_tag="PK754_06075"
     CDS             5182..6054
                     /locus_tag="PK754_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020159601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNB09103.1"
                     /translation="MFSEIIIAVTIMVGLGLLFASVLAFAYKKLRVEEDPRIDQVESM
                     LPGSNCGACGRPGCRAFAESVVGGINKPGKCTVSSPSAVASIAEYLGVAAGFEEKRVA
                     RLLCAGGKQESHNQADYYGKRTCRSVAMVTGGTKDCVWGCLGLGDCESVCDFNAITMN
                     SDGLPVVDPVQCTACNDCVDVCPKKLFTIMPVSQKLIVQCRSLLEGDAALERCSVACN
                     ACTRCVADAAPGLIQMKNNLPVIDYSQNVLARPDATRRCPTRAIVWVEGRQFEKAPAT
                     PLPIGRVEVLPEEL"
     gene            6051..9833
                     /locus_tag="PK754_06080"
     CDS             6051..9833
                     /locus_tag="PK754_06080"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNB09104.1"
                     /translation="MMHNKNNYTLYAGLTSTTTAAACFDIIASALTETASFHDESGQN
                     IFGRPLLHVSARNLGQAMGLASSGIRASATVTLQALLQDTEMMHACVRRHLPVVLVAF
                     EREQNENGEGSFCDTRARRMAGEAGWIQLIAANHQELADMIIAAHRIAELTMIPVMVL
                     ADMTRSSAGKVTFPNESALRTYLGDPDDMIETPTSGQKIIFGKIRRRIANWFHSDYPI
                     MLGADKSGLMHAFEAAAGGVYFSEAVAPIIDAAWDEFQTQFDRSYKSVETLGSEKAEQ
                     LILSGGAVIAALRSGAEQLKTAKIKAGVVNIRVWQPFPGAAVCEAISGKKNVAVLEPL
                     SGGFIQEGPLYRETISALQKAGENAGQKKSPPYPEYPSINEREKPVLFSGQFGTDVYD
                     MPEKTVAKIYEHMRTVGRRQFFIQLDFTGTKSSYPKQQIHFQNLHREFPDLNARSIGL
                     DESAKTMTAISSDIPGIVRRYEDRGAPYSRVASFYDRVGFFTESGQTKAMTADPFHAL
                     PVIPPATSAFHNATAQRAMYPVLKSESCTGCGQCAVYCPESALPMVALSTEALVQSAV
                     EQTIRAGKSLTEMTPPVIKNLAKLLHPMVSENNPSLCDLLPVAAEKLAVQMKMDSSKK
                     ENLIAQATDVGQYLAPLPAVITKTFFKTPEQYAKGSGLIFSLAVDPHACSGCGICAAS
                     CSEQALSMQPVNPASEAQLQASYRIWEALPDTPGATVKQYLDNESYNPLAALFLSRHY
                     YMAQLGSRRTDQPAEKIALHLITAAVEAATQARMLTHTSAIEKRIQNLTDKLQNILRD
                     ALPTQSTEHLLAAVSEHGTERMSLDALIGKLGSSQRFGVVDTAWIERVVYLLRDLKQL
                     HWLLTKGPTGIGRARYSAVISGNDTLAWARQFPDNPFLVPVMVFSEAIAPDFVRGIGL
                     GQMRHIIDNIKLLRRADLELQNAYLPSQHDIQIAAITWNDLTEEEKNLAPPLLLVTDA
                     TVRDAMPAGDIIALMNSGLPIKWIVTEPATGGKQNMYDRMGLNAMLFAQRQTAWAHVS
                     PGYPADMYRSITACLRSQTPAMMCVHTPMMIEKFKLQDMYAMSVESRVFPLLRYIPDA
                     KKKLAAELLHLDHNPDYQSDFVTGKNVTFADWAWFQPMWKSHFSAVSKPSSGSLDIRT
                     WLASAEERAKHTAYYEREGVFYLLSGVLAETCAVVYDAWLQLREMAGLLSAHPVKLRA
                     HWDAEWSEKHNSALDTLRSEYESKLKQQESNHLESTRIKLRDKLLALSGYQASGKQ"
     gene            9833..11191
                     /gene="rsxC"
                     /locus_tag="PK754_06085"
     CDS             9833..11191
                     /gene="rsxC"
                     /locus_tag="PK754_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNB09105.1"
                     /translation="MENMSSRLTFRHGIHPEAYKDQTADLAIERMPFVEEYAVPVAQH
                     AGAPSVPLVQKGQHVTRGDVIARPDAFVSVAQHAPVTGTVTGIELGEHPNGLLVYSIR
                     IKADPYSAQTVPQRPPLDWKNMGHKEFIAAVQSAGLVGLGGAGFPAHVKFSIPEGKTC
                     TTIIANGCECEPFLTSDHRIMVEFAADLVHGLTILRHFVPAQRVVIGVEDNKPDAIAA
                     LQEEIKKQNCDYEVVALRVKYPQGAEKMLIKAVLGTEVPSGKLPLDVGALVSNVGTLV
                     ALAQYFENAVPLIERVVTVTGPAIRKPANLMVPIGTPIRHLIDWCGGVTQDAVRILLG
                     GPMMGIAQKTLDVPVTKGISGVLVLTDNEVQELSTYQCIKCGRCIDVCPMFLNPSLMG
                     LLSRKDLYDELEEQHVMDCFECASCSFVCPSGIPLVQSFRVAKNAIRERKAKASSKPN
                     PN"
     gene            11194..12171
                     /locus_tag="PK754_06090"
     CDS             11194..12171
                     /locus_tag="PK754_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008623273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNB09106.1"
                     /translation="MSTSPFLHDEATTPWIMRQVIYALVPVAAAAYYFFGLSVILLSV
                     AAVSGCLFAEWLLSGSNPRHQSLFDGSALLTGLLLALTLPPGFPMWMAFLGGLIAIMI
                     GKYIWGGLGQNIFNPALVGRAFLQAAFPTAITTWSVPDGRYFSARGTNLAWPFFHGEP
                     LDAISSATPLSSMKFQHEATELSHLMLGHTSGSLGETCGVIILLAGVYLAYRKIFNWH
                     IPVSIIAATVVLSGILYIVNPILYPSPMHMLFSGGLMLGAVFMATDPVTSPMTVRGMW
                     IFGSGIGILVVLIRIFGGLPEGVMYAILLMNAVTPLINRVTRHRVYGTK"
BASE COUNT         3038 a         3099 c         3109 g         2901 t
ORIGIN      
        1 cggagcgtcc ggtattcgta tccgcattgg ctccaaaaaa ataaccaata tgggcgacga
       61 agccgacctg gtcgttgcat tgaatgaaca ggtgctttat gggcgcattg atcaaaaagc
      121 gtttcgcccg ggtacaaaaa tccttctgga taacaagtgg gccaaacatg atgtcaaaaa
      181 aatacaggac gaatataccg aaggtctcaa cgatctgaaa tcgcgcggat atgacgtgat
      241 cgagataccg atcgaacaag aatccatgaa gcatatcgcc gatccgcgca agggcaaaaa
      301 catgtgggtt ttcggcatgt tgtgctgcat ctatcagcgc gatacggcca aagccgaaaa
      361 tcagattcgt catactttcc gcaaaaaaaa gcaagacgtc gtggatgtca acctcaaact
      421 gttccgtgcg ggatacgcct gggccgaaca aaatctcaaa tttcaatatg acataccggc
      481 gatggcgacc gatgaacaat ttgtcgtgat gaatggtaat gaagcgatca gtctcggcac
      541 attggccgca ggcatcgaat tgtgttccat gtatcccatc acgccggcga catcggcatc
      601 gcatcatttg gctgaatgtt ttgaagcggc gggcgggctg atccatcaag cggaagatga
      661 aatcgccgca atcggttttg ccatcggcgc atcgtatgcc ggaaaaacgg cgatcacgat
      721 cacctccggg cccggcatgg cgctcaaaac cgaattcatc ggccttgcgg tcatggcgga
      781 agttccgctt gtgatcgtca atgtccagcg cggcggacct tcgaccggtt taccgaccaa
      841 agtcgagcaa ggtgatttac tctatgcgct atacggccaa cccggcgatg ccccaaaaat
      901 agttttagcc gccgcgacga tcgaagaatg ttttcacttc gtcattctcg cccggcgtct
      961 tgcggaggct ttccgcatgc cggtcatttt gctcagcgat gccaatctcg caaccggcgt
     1021 acagccatac ccgcgtccgc aaatcaatca ggagtggttt gcaccgccta ttgatcaaag
     1081 cccgtggcgc gaaggcgtac cggcgtttga ttgggatgaa gaaaccggcc tcagccgccg
     1141 cccgataccc ggccagaaaa acgggatgta cactctcacc ggattggctc atgaccagcg
     1201 cagcaaagtc gcgtacgaag cggcaaccaa tcaggtcggt tgcgatatgc gcagccgtaa
     1261 gctggccaca tttctccaat cactcaaacc gccggtcgtc aacggcgatc ccgaaggtga
     1321 tctcctgatc gtcggctggg gttcgaccct cggtgcgatc gaagaagccg tcaatcatgc
     1381 ccgtgaattg ggcggtcgcg tatcgtctct gcatctgcgt ttcctctcgc cgatagaacc
     1441 gggtctcaaa gatattttca aacgattcaa aaaagtcatg acggtggaga tcaactattc
     1501 ggataatctg aatgatccgc tgatcacgcc ggaaaannnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnaaaa tcatataaag gagcactccc gtgtttggat taaaatccga
     1681 ttgcgtcctc gatatacctg aggattatta cacgttagcc gactatgaaa gcgcaacggc
     1741 acgctggtgt ccgggatgcg gcgatcacgg cattttgacc gctgtacaaa gactgtgcag
     1801 cgaaaaacaa ttaccgcccg aaaaaacagt atttgtgtcc ggcatcggct gttcgagccg
     1861 tctgccgcat tatatgaaaa cctacgggtt tcacggtttg cacggacgtg cacttcccgt
     1921 agccaacggt atcaaatttc gccgcccgga tctgcatgtc tttgtcgcca ccggcgacgg
     1981 cgattgttgt tccatcggta cggcgcattg gattcacgcc attcgctata atacgaatat
     2041 caccgtgctg cttttagaca acgaaattta tggattgacc aaaggacaag cttcaccgac
     2101 ttccaataaa ggtacagttt ccaatacctc accgcgcgga tcgcaattac ctccgatcaa
     2161 tccgatacaa accacgctcg gcgtctccaa tgtttccttc gtcgcacaga cggtagattg
     2221 gaatccggcg catctgtacg ctacgatcga agccggttac aatcatcccg gtttgtcgtt
     2281 tatccgcgtt ttacaacgtt gcccggtttt ccaaaaagat ctttttcaaa caaccatgag
     2341 cagccccgat gcgatcacac ttttgacgca taaaaacgga attcaagttg accccgcgat
     2401 cacaaaaata tataaaaacg tggtcgagca tgatccgtcg gatatcaacc aggccagaac
     2461 tttggcgggc aatcatgacc gcgaagtgat cggccttttg tatcagaata agaacgccga
     2521 acggtatgat gattacggtg cgcacaatct cggcatttca ccggaggaca aaatgcgcgc
     2581 gatccacaag gaattagaca aatttgcggt ttgaaatttt atgaaaaatg aggaagctcc
     2641 catgacgagc cctgatccta tgtttgccca tctcgatcac gtgttgcgaa acacgtcgac
     2701 gcacggtacg caagaccata cccgtgcgga acaaaaatcc gaaaaagcgg cgaaaccatt
     2761 tatcccggaa tccaaagcgg cagacctttc gcagacgtca ctgttctgga aaaatctccg
     2821 tgcgttttat cgcggtgacg cctccggcgc gatcgccaaa aaccggttgc cggcattgct
     2881 cttaccattc cgcgataccg atcgcattcg gtttgattat cccgtttgga ttgacgtttc
     2941 atcggatgac gagaaacccg ttcgcacgct caatcaactt ttggaagacg cgatagtatc
     3001 ttttgcaccg ggcgcggacg atgccaaaat actcaaagac aaccggctgc ggttagaaca
     3061 aatgatgcgc caatatctga gtgaaaacgg cggctccgcc gcatgtgctt cgatctggga
     3121 aacagcagtc acgcggctta ccgaacaatt gcatctcaaa ggcgaaacag aaaaatcttt
     3181 tttatcggac acgaagcgtc tgaaagatca tttgcccaag gacgggcacc tttatgattt
     3241 ttcgaaagcc atggcgctta cattgctttc atcggcactc cgtcatcgtg tcgcggatgc
     3301 acgtacccgg cttctcaaag acgccgctgt tttgcgtcag cggttgcaag aaatgattct
     3361 catcgaagac cacagaaatc ctgaagcaaa aaattcatca gccgcacccg gcaatcatgg
     3421 ttacggcagt gcgtttgttg attttaccgc ggtatcacgc gttttgccgg catcgccgac
     3481 cttgccgatg gcgccggcac gacgcaaacg gataaccgaa gcattgaata tcctcagcca
     3541 gcccgataat attttcaaag aatcggcatt acttatcgtc acgccggaca tcgaagcgcg
     3601 ttacggtatg gcatggaccg acactatgac ctccgcacgg atatccaaag cgcccagtgc
     3661 tgaggcgctg cgattggccg aagcgcagtt tgatgcgtac atgcaaacga taacctcgtg
     3721 ggttcgtgcc aaacgcatcg cgcaacttga atcgcaagat cgttatgagg ccgacgtaca
     3781 cgatgtgtat tttgaaaaat ttgactggcg tgcattgagc gatgaggagg tggctttgtg
     3841 tgcgccggtc gtcgttttag cttcggatga agaaatcctg cgtgaccatc ttcatgattt
     3901 ttcgacagcc ttagcttcgc ggcgtccgat caaatttttt gtcatgcaat cacgatacca
     3961 cagtgaccaa gcggcgccga caggtgttat ctcatttgct catgatctgg ccgcgatggc
     4021 ggtcgcacat cgccaggcgt atgtgtatca ggccgcgatg gttcatcccg agcaagtgtt
     4081 tcacggtatc cgcaccggat tgtctcaatc ggcaccatcg ttgttttata tgttggcacc
     4141 ggtatcggaa cacgcactgc tggcatcggg ttccgcatta gaaagccgcg ctttccccga
     4201 atattcctac gatccgcaac gcggcgttca atggggcagc cggtttagtg tggaagccaa
     4261 tccccaagct gacagcgact ggccggtgta tgaattgacc gccacgcaat atgataaaac
     4321 cgaaaccttg ctgcgattgc ctttcacgtt tgccgatcat cttgtgttcg atgacacgat
     4381 gacgcatcac ttcatggatg tgccgccttc gcactggaca ccggatcttg ttttactcgc
     4441 ggattatctc gcgctgcctc catcggaagc atataccaaa attccctatg tgtggactgc
     4501 cgacaaagac ggggttttac acaaaacggc ggttgatcat gcgctcgtat tgatggcgca
     4561 ggatcgtctg gatgcatggc attttattca ggaattcggc ggcatcaaca gttatcacgt
     4621 tgcgcgggcg attgcaaaaa cccgaactga aatgacggct gaaaaagaac gggcactgga
     4681 cgcgctgcat gtgcttcatc aaagtgaaat tgaaaaagtg cgtgaagaaa ccgcgcgcag
     4741 tgcgatggaa aaattatcag cggtgctgtt ggatctggat actttgcctg tgcacaacac
     4801 gccttcatcc gtaccctctt taagtgtgtc caaagctgaa acatcggcta cggtgtccga
     4861 tacaccgacg gtatctgcac cggtggagga agaaccggtc atatccaacg aagcttggct
     4921 tgaaacgata cgttgtacaa gttgtaatga atgcatcaac ctgaatccga atatttttaa
     4981 gtataacagc gctaaacaag cgtatatcgg cgacgcccgt gccggaagtt atttgcatat
     5041 cgtgcaagcg gccgagaaat gtccggctaa atgtattcac cccggattac cgctcaatcc
     5101 ttcggaagac ggattggatg cgttgatcca gcgggctgcc gtttttaact aaaaactttt
     5161 gttacgtaag aagaatttat cgtgttttcc gaaatcatca tagctgtaac gatcatggtc
     5221 ggtctcgggc tgcttttcgc atcggtacta gcgttcgcgt ataaaaagct gcgtgtcgaa
     5281 gaagatccgc gcattgatca ggtcgaatct atgttaccgg gttccaactg tggcgcatgc
     5341 ggtcgcccgg gttgccgtgc atttgcagaa agtgtcgtcg gcggcatcaa taaaccgggc
     5401 aagtgtaccg tcagttcacc ttcggcggta gcatcaatcg cggaatacct cggtgtggcc
     5461 gccggctttg aagaaaaacg cgtagcacgt cttttatgtg ccggcggtaa acaagaaagc
     5521 cacaaccagg ccgactatta tggaaaacgc acctgccgca gtgtagcgat ggttaccggc
     5581 ggaacgaaag attgtgtatg gggatgtttg ggcctcggag attgcgaatc ggtctgcgat
     5641 tttaacgcga tcacgatgaa tagcgacggt ctaccggtcg tcgatcccgt ccagtgcacg
     5701 gcgtgcaacg attgtgtgga tgtatgcccc aaaaaacttt ttaccatcat gcccgtcagt
     5761 cagaaactga tcgtacagtg ccgttcactg cttgagggcg atgccgcatt ggaacggtgt
     5821 tcggtagctt gcaatgcgtg cacccggtgt gtggccgatg cagcgccggg tttgatccag
     5881 atgaagaata atttgcccgt gattgattat agtcaaaatg tattagcgcg ccccgacgcg
     5941 acgcgccgtt gcccgacacg agccattgtg tgggtggaag gtcgtcaatt tgaaaaagcg
     6001 cctgcaacgc cgctaccgat agggcgtgtg gaagttttac ctgaggaact atgatgcaca
     6061 ataagaacaa ttatactctt tacgccggtt tgaccagcac cacgacggcg gcagcgtgtt
     6121 ttgatatcat cgcatcggct ttgacggaaa cggcttcctt tcacgacgaa agcgggcaaa
     6181 atattttcgg aagaccgcta ctgcacgtat cggcccgcaa tcttgggcag gcgatgggat
     6241 tggcttcttc gggtattcgc gcctctgcaa cagttacact tcaggcgctc cttcaagata
     6301 cagaaatgat gcacgcctgc gttcgtcgtc atttgcccgt agtactcgtg gcatttgaac
     6361 gtgaacaaaa tgaaaacggc gaaggatcgt tttgcgatac acgtgcacgg cgtatggccg
     6421 gtgaagccgg atggatacaa ttgatagcgg caaaccatca ggaattggcc gatatgatca
     6481 tagcggcgca tcgtatagcc gaactgacga tgattccggt aatggtttta gccgatatga
     6541 caagatcatc ggcgggcaaa gttacttttc caaatgaaag cgcattacgc acttatcttg
     6601 gcgatcccga tgatatgatc gaaacgccga catccggaca aaaaataatt ttcggaaaaa
     6661 tacggcggcg tatcgccaac tggtttcata gcgactatcc gattatgttg ggcgccgata
     6721 aatccggcct gatgcatgcg tttgaagcgg cggccggcgg cgtgtatttc tcggaagcgg
     6781 tcgcaccgat catcgatgcc gcgtgggacg aatttcaaac acagtttgat cgctcatata
     6841 aaagcgtcga aactttaggt tcagaaaaag ccgaacagct catcctctcc ggaggcgctg
     6901 tcattgccgc acttcggtcc ggtgcagaac aactcaaaac cgccaaaata aaagccggtg
     6961 tagtgaatat ccgcgtgtgg cagccctttc ccggtgccgc cgtttgcgaa gccatcagcg
     7021 gtaaaaaaaa tgtggccgtg cttgaacctc tgtccggagg atttattcag gaaggaccgt
     7081 tatatcgcga aacgatttcg gcattacaaa aagccggcga aaatgccggg caaaaaaaat
     7141 ctccgccgta cccggaatat ccgtcaatca atgaacgtga aaaacctgtt ttgttttccg
     7201 gtcaatttgg cacggacgtt tatgacatgc cggaaaaaac agtcgcgaag atttacgaac
     7261 acatgcgcac ggtcggacgc cgtcagtttt ttatacaact tgattttaca ggaaccaagt
     7321 cgtcgtatcc gaaacaacaa attcattttc aaaacctgca tcgcgaattt ccggatttga
     7381 atgcgcgttc gataggactc gatgaatcgg ccaaaacaat gactgcgatt tcgtctgaca
     7441 ttcccggtat cgtacgccgc tacgaagatc gtggcgcacc gtattcgcgt gtggcttcat
     7501 tttatgatcg tgtcggattt tttacagagt ccggtcaaac taaagccatg acggctgatc
     7561 cgtttcatgc cttaccggtc atacccccgg cgacgtcggc atttcacaat gccacagcac
     7621 aacgggcgat gtatccggtg ttaaaatcgg agtcatgcac cggttgtggt caatgtgcgg
     7681 tatattgccc tgaatccgct ctgccgatgg tagctctttc gacggaagcg ctcgtacaat
     7741 cagctgtcga gcaaacgata cgggccggaa aatcgctgac agaaatgact ccgccggtaa
     7801 tcaaaaatct cgccaaactg ttgcatccga tggtatcaga aaacaaccca tcgttatgcg
     7861 acctgcttcc tgtggccgcc gaaaaattag ccgtgcagat gaaaatggat tcatccaaaa
     7921 aagaaaattt gatcgcgcaa gcgaccgacg tcggtcaata tcttgccccg cttccggctg
     7981 tgattaccaa aacatttttc aaaacaccgg aacaatatgc taaaggcagc ggcttgattt
     8041 tttcattggc cgtagatccg catgcttgca gcggatgcgg catttgtgct gcatcgtgtt
     8101 cggaacaagc cctaagcatg caaccggtga atccggcgtc cgaagcgcag ttacaagcgt
     8161 cgtaccggat ttgggaagcg ctgccggata cgcccggagc cacggtcaaa caatatttag
     8221 ataacgaaag ttacaacccg ctggccgcct tatttctgag ccgtcattat tatatggcac
     8281 aactcggcag ccgacgcacg gatcaaccgg cggaaaaaat cgcgttgcat ctgatcacgg
     8341 ccgctgtcga agcggccaca caggcacgca tgcttacgca cacgtctgca atagaaaagc
     8401 gtatccaaaa tcttacggat aaattacaaa atattttgcg cgacgcttta ccgacacaaa
     8461 gtaccgaaca tttgttggcc gccgtttccg aacacggcac ggaacgcatg tcgcttgatg
     8521 ccttgatcgg aaaactcggt tcctcgcaac gatttggcgt cgtcgatacg gcgtggatcg
     8581 aacgcgtcgt atatcttctg cgcgacttga agcaactgca ttggcttttg acgaaaggac
     8641 ctacaggtat cggacgtgcg cggtattcgg cggtaatcag cggcaatgat actctggcat
     8701 gggcacgtca atttccggat aatccgttcc tcgtgccggt catggttttt tcagaagcca
     8761 tcgctccgga cttcgtccgc ggtatcggcc tcgggcaaat gcgccacatc attgataata
     8821 tcaaactttt acggcgtgcc gatctcgagt tacaaaacgc gtatttaccg tcacaacacg
     8881 atatacaaat cgcggccatc acttggaatg atcttacaga agaagaaaaa aatcttgcac
     8941 cgcctttgct tttagtgacg gatgcgaccg tacgggatgc catgccggcg ggtgacatca
     9001 tcgcgcttat gaatagcggc ctgccgatca aatggatcgt cactgagccc gccaccggtg
     9061 gcaaacaaaa tatgtacgat cgtatgggat tgaatgccat gctttttgcg caacgtcaaa
     9121 cagcgtgggc tcatgtatca cccggctatc cggccgacat gtaccgcagt attacagctt
     9181 gtctgcgcag ccaaacgccg gccatgatgt gcgtgcatac gccgatgatg atagaaaaat
     9241 tcaaactgca agacatgtac gccatgtccg tagaatcccg tgtatttcct ttgttgcgct
     9301 acatccccga cgcgaagaaa aaattagcgg ctgaattatt acaccttgat cacaatcccg
     9361 attatcaatc ggattttgta accggaaaaa acgtgacctt tgccgactgg gcctggtttc
     9421 agccgatgtg gaaatcgcat ttttcagcgg taagcaaacc ctcttccgga agtttggata
     9481 ttcgtacatg gttggcttcc gctgaagagc gtgctaaaca taccgcgtat tatgaacgcg
     9541 aaggcgtgtt ctatttacta tcgggcgtct tggctgaaac gtgtgcggtc gtctatgatg
     9601 cgtggttaca acttcgcgaa atggccggtc ttctttcggc ccacccggtc aaattacgtg
     9661 cgcattggga tgccgaatgg tctgaaaaac ataattcggc gcttgataca ctgcgtagtg
     9721 aatatgagag caaactgaaa caacaggaat caaatcatct ggaatccacg cgcatcaagt
     9781 tgcgggataa gttattagcg ctttccggat atcaagcaag cggaaaacaa taatggaaaa
     9841 catgtcgtca cgattgacgt ttcgccacgg gattcatccc gaagcttata aagatcagac
     9901 tgccgatctt gcgatcgaac gcatgccctt cgttgaagaa tacgccgttc cggttgcgca
     9961 gcatgcgggc gcaccgtcgg tgccgcttgt acagaaaggc caacacgtta cacgcggcga
    10021 tgtgatcgcg agaccggatg ctttcgtatc cgtagcgcag cacgcgcccg tcacgggtac
    10081 cgtcacgggt atcgaacttg gcgaacatcc caacgggttg ctggtgtact ctattcgtat
    10141 caaagccgat ccgtacagcg cacagaccgt accgcagcga ccgccgttgg attggaaaaa
    10201 tatgggacac aaagaattta tcgccgctgt acagagcgcc ggtttggtag gccttggcgg
    10261 cgccggattt cccgcgcatg tgaagttttc catccccgaa ggaaaaacct gtacgacgat
    10321 cattgccaat ggctgcgaat gcgaaccgtt tcttacgtcc gatcatcgca tcatggtaga
    10381 gtttgcggcc gacttggttc atggcctgac gatactcaga cattttgttc ccgcacagcg
    10441 ggtcgtgatc ggcgtggaag ataacaaacc ggatgcgatc gccgcgttgc aggaagaaat
    10501 caaaaaacaa aattgcgatt acgaagtggt agcgctccgg gtcaaatatc cacaaggtgc
    10561 agaaaaaatg ttgatcaaag cggtactggg aacggaagtg ccgtcgggta aattaccgct
    10621 ggatgtcggc gcactcgtgt ccaatgtcgg tacgttggtg gccttggcgc aatattttga
    10681 aaacgccgta ccgttgatcg aacgcgtagt gaccgtgacc ggcccggcta ttcgcaaacc
    10741 ggctaaccta atggtgccga tcggtacgcc gattcgccat ctgattgatt ggtgcggcgg
    10801 cgttacgcaa gatgccgttc gaattttgct cggcgggccg atgatgggca tcgcacaaaa
    10861 aacattggat gtacccgtaa ccaaaggtat ttcgggcgta ctcgtcctga cggataacga
    10921 ggtgcaggaa ctttccacat accaatgtat caagtgcggg cgctgtatag atgtgtgccc
    10981 gatgtttctc aatccttccc tcatgggtct gctctcacgc aaagatttgt atgatgaact
    11041 ggaagaacag cacgtgatgg attgttttga atgcgcgtcg tgttcgtttg tctgcccttc
    11101 gggaattcct ttagtacaga gttttcgggt tgctaaaaat gctatacgtg aaagaaaggc
    11161 gaaagcgtca tcgaaaccca atccaaatta acactgtcca cgtcgccgtt tttgcatgac
    11221 gaagcgacga cgccttggat catgcgtcaa gtgatttacg cgttagtgcc ggtagcggca
    11281 gccgcgtatt attttttcgg actgagtgtg attttactgt cggttgccgc cgtgagcgga
    11341 tgtttgtttg ccgaatggct tttgtccgga tcgaatccgc gtcaccaatc actcttcgac
    11401 ggcagcgctt tattgaccgg gcttttgctg gcattgactt tgccaccggg ttttccgatg
    11461 tggatggcat ttctcggtgg actgatcgcg atcatgatcg gtaaatatat atggggcggg
    11521 ctcggacaaa atatttttaa tccggcattg gtaggccgtg cttttctgca ggccgcattc
    11581 cccacggcga tcacgacgtg gtcggtaccg gacggacgat atttttccgc acggggtact
    11641 aatctggcat ggcctttttt tcacggcgaa ccgttagacg caatctccag cgccacgccg
    11701 ctttccagta tgaagtttca gcatgaagcc accgaattat cacatcttat gctgggccat
    11761 acctccgggt cgcttggcga aacctgcggt gtgattatac ttttagccgg cgtatatctg
    11821 gcctatcgaa aaattttcaa ctggcacatc ccggtttcta tcatagctgc taccgtcgta
    11881 ctgtcgggca ttttgtatat cgtcaacccg attttatatc cttcaccgat gcacatgctt
    11941 ttctccggcg gtttgatgct gggcgccgta tttatggcca ccgatcctgt gacgtcgccc
    12001 atgacggtgc gcggcatgtg gattttcgga agcgggatag gaattttagt cgtgctcata
    12061 cgcatcttcg gcggtttgcc ggaaggcgtc atgtatgcca tattgctgat gaatgccgta
    12121 acgccgttga tcaaccgcgt tacgcgccat cgtgtttacg gaacgaaata aaataaatta
    12181 tggaacatca acatataccc gcaacacccg ttgcgacgaa agaagccgaa cccagctcat
    12241 tgatttt
//
LOCUS       DAOQNQ010000066        12132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1856_length_12132_cov_6.005548, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000066 DAOQNQ010000000
VERSION     DAOQNQ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12132
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1856_length_12132_cov_6.005548"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2878
                     /locus_tag="PK754_06095"
     CDS             <1..2878
                     /locus_tag="PK754_06095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB09107.1"
                     /translation="DNTSLSVFAITEDLQHHLWVGTQQAGLFRKSVSTADWRQYRFDP
                     ADPRSVSSNDIFVLYTDDIGRVWIGTRDNGLNRYDAMADNFIRYQPTTGNPYSISGLS
                     IRSIRQYKTEIWIGTLNNGVNRLDPLTGRFNSYQPDASKPGSITHNSIWSLFADSKDI
                     LWIGTFGNGVMQYDPAGDTFVQIQPKPSSTPRNDQVVVSILEDRSGMMWFGTIGDGLR
                     KYLRIKDRFQFINAHPNPKRGLPDNMIWSLLEDRQSNIWISTQKGITVYNPKKDVFSQ
                     YTNPRREAWKLGETAYAMAQDSNGVIWFATFGDGLKRFDPRTQRISAFQVNKFSPGGI
                     PTNNILALYVDHENVLWVGTYDAGLLRLEPGSRTFKVFKKKEKDSTSLQDNQVRTVFE
                     DARGVLWVGTAQGGLHRFDRNSSTFKVYSTQSPGLKRLQNNFVSAVSEDKDGTLWVAT
                     YGGLHRMNRETETFICYSEKNGLASNQIYALIKDQRGRFWMTTSHGLSVLDPHDAVPV
                     FRNYTSHHGLQSDEFNMGAAIRNVSGTIMAGGVSGVVIFHPDSLESKKYDVPVAITAV
                     RVANRMLGYEPWDDKYTTLRHDQSTLAFEFASFDFSFPEQNKYAYRLEGFETEWSDAD
                     ASRRTVNYTNLDPGKYVFQVRGTNSDGHWSDKTAAFRFEILPPFYKTWWFVGSSIFLI
                     VFLAILFYRIRIRRIENQKNELARQITERTRELREKNSELERVNAIRNELLGMAVHDL
                     RSPIAAIRNLVQFHLEALKNQINDRETDMHDMERALSLSDRISSTLNDLLDISAIESG
                     KLSITKLPENIETIIHDAFELNLRAAERKGTTLKSDIAPNLPKILVDRIRIADVLNNL
                     ISNAVKYTPKNGRIRVYAEISGMFMHIHVEDNGQGLTQEDLSSAFKTFTRLSATPTDG
                     ETSTGLGLAIVKKIVELHGGSVWVRSEKNKGSTFSFSVSLEGHH"
     gene            3011..4096
                     /gene="mqnC"
                     /locus_tag="PK754_06100"
     CDS             3011..4096
                     /gene="mqnC"
                     /locus_tag="PK754_06100"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HNB09108.1"
                     /translation="MLQKIFDKIENNERLTFEDGMTLYACKELPLLGFLANHIRRKKR
                     PGNEVTYIIDRNINYTNVCVDLCSFCAFYRPIGHDEGYVLPTEVIDEKIDELIALEGI
                     QILMQGGLNPKLKITYYEDLFRHLRSKYPQIWLHCLSAPEIRYIAKVSGLTLDETVAR
                     LKAAGLQSVPGAGAEILVDRVRSEISRGKCDTQEWLDAHSIFHKHGLTSTATMMYGHV
                     ETREERIEHILKIRDLQDNSLKNYGLGFTAFIPWNFQPDNVPLGKNQNLRTTTTVEYL
                     QTLAISRLLLDNIDNFQVSWVTQGQDIGQLGLNYGANDFGSLMIEENVVSQNGTSFNV
                     TVKDIEKMIRDAGYEPKRRNNQYTFVN"
     gene            4196..4654
                     /locus_tag="PK754_06105"
     CDS             4196..4654
                     /locus_tag="PK754_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNB09109.1"
                     /translation="MTKLSSKDVLRILDLQPHPEGGAYRETYRSKNPAKVDPQRSVCT
                     AIYFLVQAHQKTEWHRVKNDEMYHHYAGATLDVLLVSPDGVFEKHKLGDDLETGARPQ
                     LLIPGGYWQSVQAHDSWVLVGCTVSPGFDFSDFELSNEAALKKQFPHLSF"
     gene            4666..5631
                     /locus_tag="PK754_06110"
     CDS             4666..5631
                     /locus_tag="PK754_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNB09110.1"
                     /translation="MTTQEVFINKNYQMRAGWRVLIFIVAFILISMPSVYLGLLFTKF
                     LEFPKEYNEIIVKFLTTIIPLGLSFFLLKKLDNRSTDYLGLKTDCAGFIELAWGLLIG
                     FVMLTIAVLIPYAMGYVSLQFATEPPSYFFSGLAFNLLLFIVVGFNEEILFRGYIFQA
                     TGEGLGFVGATLLYSLLFGAAHLGNPNVSLFGIINIVLAGVLLSLAYIKTRALWMPIG
                     IHIAWNFTQGYIWGLPVSGTTVIQPMIIAQEIGPDEITGGTFGPEGGAMCSIVCIGAC
                     LLIWKYFSPSDAMKQVIQEAETAQPFKPNISESIGSNTNPNNGIS"
     gene            5769..6869
                     /locus_tag="PK754_06115"
     CDS             5769..6869
                     /locus_tag="PK754_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB09111.1"
                     /translation="MNRNIFFLGVLFFSSFLFAHDGDIHKDSSSTTVRTITNIAPNIY
                     MIRHPDAPDGFPQGNTTVIIGDREVLVVDACYLPSSAKQDIEQIQKWTSKPVRYLVNT
                     HWHYDHTMGNGTYAEAFPGISIIAHHETKRIIQGYNPGWFARFPNRSAIFKSAIESGK
                     DANGNKLSESDIRDYKKYLDGLTPVQEEFSKIKDRAPNLSFDHELTIDLGNREVQIKH
                     LGRGNSAGDIILYLPKEKIIVIGDLMDHPVPYLGSGFPGEQVETLNKIAQLDADTFIP
                     GHGNVLTGKSYLFLLRDFLAEVVAAVRSEIEKVGNNPRLIDQVRESVHKRVDFTAWKK
                     RFVGDNKEDGDFFDSFSVQGVFNCAFNELVRK"
     gene            7251..8279
                     /gene="queA"
                     /locus_tag="PK754_06120"
     CDS             7251..8279
                     /gene="queA"
                     /locus_tag="PK754_06120"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNB09112.1"
                     /translation="MKLADFKFKLPEKYIAQYPVEPRDKAKLMVLDRKTGDIEDKIFR
                     DITEHITKNDCLVLNETRVFPARLMARKEKTNAKVEVFLLRELENNLWEVLVKPARKV
                     RIGNRLVVDGVYCDVIDNTVSGGRVVRFIYPQADFHKFIDKVGQCPLPPYIKRSAEES
                     DKEDYQTVFARVRGSVAAPTAGLHFTKELLGRLKRKGVTIVPLVLHVGLGTFRKVQVE
                     DLSRHKMDSEYFEIPEITARAINQTKDKGGKVIAVGSTVVRSIESAVTSLNRVKPHRG
                     WTDKFMYPPYEFKIVDKMVTNFHTPESTLLMLAAAFGGKDNIFKAYKKAMKDNYRFYS
                     FGDAMLIE"
     gene            complement(8351..8773)
                     /locus_tag="PK754_06125"
     CDS             complement(8351..8773)
                     /locus_tag="PK754_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNB09113.1"
                     /translation="MQNPYTQVTIEATIQASLEKVWSVWTSPEHIVKWNAASDDWHTT
                     KAENDLRVGGRFLSRMEAKDGSFGFDFTGSYDEVVQYELIAYTMEDGRKVRVHFSQSG
                     NSTKIVSVFDAEQENPVDMQKNGWQAILDNFAKYVVAV"
     gene            complement(8847..9491)
                     /locus_tag="PK754_06130"
     CDS             complement(8847..9491)
                     /locus_tag="PK754_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNB09114.1"
                     /translation="MKIGVLGSGQVAQVLASGFIKHGHEVMMGTRDVTKLSEWKSKNG
                     DKAQVGSMATAASFGELIVLAVKGHAAEDAVKSAGITNLKNKTVIDVNNPIKEVPPVN
                     GVLQFFTGPNESLMEQLQVLAPEANFVKAFSCVGNALMVNPKMAEGTPTMFICGNNDA
                     AKAQVTAILAQFGWDWADMGKVEAARAIEPLCILWCIPGFTSNSWMHAFKLLKK"
     gene            complement(9587..11380)
                     /locus_tag="PK754_06135"
     CDS             complement(9587..11380)
                     /locus_tag="PK754_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB09115.1"
                     /translation="MKLLIGYLKNYWKLVALALFLAAVNQIFSLLDPWIFRHVIDEYA
                     TRYKDYSFDEFVKGVSVLLGAAIGVAFVSRVAKNFQDYYINTITQKLGAQIYSDGLRH
                     SLELPYSTFEDQRSGETLGKLQKVRTDVERLISAFINILFTTLVGIIFVTAYAISVHW
                     VIAPIYFSTIPIIGWVSSVLSKKIKVVQKAIVAETTALAGSTTESLRNIELVKSLGLA
                     QQEIERLNGTTKKILKLELKKVKYIRSLSFVQGTLVNFLRNSILFIMLYLIFAQEITF
                     GEFFSLFIYSFFIFGPLQELGNVINIYREAEVSLKNFEDILNTPCEAKPANPVLIADI
                     EKLEFENVVFKHQSAQRHALDGISFSVHRGETIAFVGPSGAGKTTLVKLLVGLYKPQS
                     GIVRYNGHGGDTIDLDVLREQIGFVTQDTQLFAGTIRENLLFVNPKATDQECLDVLNK
                     AACGSLLARADKGLDTVIGEGGVKVSGGEKQRLSIARALLRRPRLLVFDEATSSLDSL
                     TEEEITHTIRDISHRQEHMTICIAHRLSTIMHADRIFVLEKGRIGEAGKHDELLAQKG
                     LYYAMWRQQIGENKSDMTVKNMMEVNSVRKN"
     gene            complement(11415..11639)
                     /locus_tag="PK754_06140"
     CDS             complement(11415..11639)
                     /locus_tag="PK754_06140"
                     /inference="COORDINATES: protein motif:HMM:NF022833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HNB09116.1"
                     /translation="MKFNKKHSQVLFVLFMALTLSCIMSLVMTFRTFGMETWHVALWL
                     QAWGFSFLVAFPVAFIAVPRIRRLVEMITE"
     gene            complement(11654..11950)
                     /locus_tag="PK754_06145"
     CDS             complement(11654..11950)
                     /locus_tag="PK754_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09117.1"
                     /translation="MNQYMVDVSLPEFPDEAFFSKIPEQRTLINRLLEERVVQSYALS
                     ADRTKLWIVIRAQSDSEAFDILATFPLYDYFRFDIYELAFHNKATVRSTLFSMN"
BASE COUNT         3423 a         2796 c         2571 g         3342 t
ORIGIN      
        1 ggataataca tcattgtccg tctttgcaat tacggaagat ttgcaacacc atctttgggt
       61 tggcacacag caggccgggt tattcagaaa atctgtatca acggccgatt ggcgtcagta
      121 tcgttttgac cccgcggatc cccggtctgt gagcagcaat gatatttttg ttttatacac
      181 ggatgatatc ggacgtgtct ggattggaac acgggacaat ggtctcaatc gctacgatgc
      241 gatggcggat aattttattc gttaccaacc cactacagga aatccatata gcataagcgg
      301 tttatccatt cgcagcatcc gtcagtataa aacggaaatc tggattggca cactcaataa
      361 cggagtcaat cgtcttgatc cgctcaccgg acgattcaat tcgtatcaac ctgatgcttc
      421 caaacccggt tccataactc ataacagcat ttggtcctta tttgcggaca gtaaagacat
      481 tttgtggata ggtacatttg gcaatggtgt tatgcaatat gatcctgcag gagatacctt
      541 tgtccaaatt cagcccaaac cttcctcgac accgcgcaat gatcaagtcg tagtttctat
      601 tctggaagac cgcagcggca tgatgtggtt tggcactatc ggtgatgggt tgcgcaaata
      661 ccttcgtatc aaagaccgct ttcaatttat caacgcacac cccaatccta aacgcggttt
      721 accggataat atgatctggt cactgctcga agatcggcaa tccaatatat ggatcagtac
      781 tcaaaaaggc atcacggtct ataaccctaa aaaagatgtt ttttctcaat ataccaaccc
      841 gcgaagggaa gcgtggaaac tcggcgaaac agcctacgca atggcacagg acagcaatgg
      901 tgtgatttgg tttgcgacgt tcggagacgg tctcaaacgg tttgatccgc gcacacagcg
      961 catttcagcg tttcaagtta ataaattttc gccgggcgga attcctacaa ataatatttt
     1021 agcgctatac gttgaccacg aaaacgtact ttgggttggt acgtacgatg cagggcttct
     1081 ccgattggaa cccggaagca gaactttcaa agtttttaag aaaaaagaaa aagattcaac
     1141 atcgctgcaa gacaatcagg tgcgtaccgt ttttgaagac gcgcgcggcg ttttatgggt
     1201 aggcacggcg caaggcgggc tacatcgttt cgacagaaat tcgtctacgt ttaaagtata
     1261 ttcgacgcaa agtccgggac tgaaacgtct tcaaaataat tttgtcagcg ccgtatccga
     1321 agacaaagac ggtactctgt gggtggccac ctacggagga ttgcaccgga tgaaccgcga
     1381 gacggaaaca tttatttgtt actccgaaaa aaacggactc gcaagtaacc aaatatacgc
     1441 attgatcaaa gatcagcgcg gtcgtttttg gatgaccacc agccacggtt tatccgtact
     1501 ggatccgcac gatgccgtac ctgtatttcg aaactacaca tcgcaccacg gcttgcaaag
     1561 tgatgagttt aatatgggcg ctgcgattcg caatgtatcc ggaacgatca tggcgggcgg
     1621 tgtgagcggc gtggtgattt ttcatccgga tagccttgaa agtaaaaaat acgatgtccc
     1681 ggtggctatc acggccgtcc gtgttgccaa tcgcatgttg ggatacgaac cttgggatga
     1741 caaatatacg acgcttcggc atgatcaatc taccttggct tttgagtttg catcgtttga
     1801 tttttctttt ccagaacaga ataaatacgc ctatcgcctt gaaggatttg aaacggagtg
     1861 gtccgatgcg gatgcatccc gacgtacggt caattatacc aatcttgatc ccggaaaata
     1921 tgtttttcag gtacgcggca ccaatagcga cggccattgg agtgataaaa cggccgcatt
     1981 tcgttttgaa attctgccgc cattttataa aacatggtgg ttcgtcggca gcagtatttt
     2041 tctgatcgtg tttttagcta ttctatttta tcgaatacgc atccgccgca tcgaaaatca
     2101 aaaaaacgaa ttggctcgcc agataacgga acgcacaaga gagttgcgtg aaaaaaacag
     2161 cgaattggaa cgcgtcaatg cgatacgaaa cgaactttta ggcatggccg tacatgactt
     2221 gcgtagtccc atcgctgcga tacgaaatct ggttcagttt cacttggaag ctttaaaaaa
     2281 tcaaattaat gatcgtgaaa cagatatgca tgatatggaa cgagcgctaa gtttatccga
     2341 tcgcatcagc tcgacattaa atgatctgtt ggatatttcc gctatcgaat cgggcaaatt
     2401 atccataacc aaactccccg agaatatcga aaccattatt catgatgcgt tcgagctcaa
     2461 tcttcgtgca gccgagcgca aaggtactac gttaaaatcc gatatcgcac ccaaccttcc
     2521 caaaattctt gtggatcgca ttcggatcgc cgacgtactc aacaacctca tcagtaacgc
     2581 cgtcaaatac acacctaaaa acggtcgcat ccgtgtttat gccgaaatat cgggcatgtt
     2641 tatgcacatc catgtcgaag acaatggcca gggcttaaca caagaagatt tatccagcgc
     2701 tttcaaaacg ttcacgcgat tgagcgccac accgaccgat ggtgaaacca gcaccgggtt
     2761 agggctggcc atcgtaaaaa aaatagtcga actccacggc ggctcagttt gggtgcgttc
     2821 tgaaaaaaat aaaggctcca catttagttt ttccgtttcg ctcgaaggac accattaatc
     2881 cgctcaggac gatccttttt tctgtgcgcc gcgttacttt gaaatcctcc ttgcaatcca
     2941 tttcggtttt ttctaatatg gcgcgttcca agtttcaaaa ataattgaaa ctttaaatga
     3001 aagcgaattt atgctgcaga agatttttga taaaatcgaa aacaacgaac gcctgacgtt
     3061 tgaagacggg atgacgctgt acgcttgcaa agaactgccg ctgctcggtt tcctcgccaa
     3121 ccatattcgc cgaaaaaagc ggccgggtaa tgaagtcacg tatatcattg atcgtaatat
     3181 taactatacc aatgtttgcg ttgatctgtg cagtttttgt gccttttatc gcccgatagg
     3241 tcacgacgaa ggttacgtcc ttcctacgga agttattgat gaaaaaattg acgaacttat
     3301 cgcactggaa ggcattcaga tccttatgca aggcggactg aatccaaaac tcaaaatcac
     3361 ttattacgaa gatttatttc gtcatctgcg ctccaaatac cctcagattt ggctgcactg
     3421 cctgagcgcc ccggaaatcc gctacatagc caaagtttcg ggtcttacac tggatgaaac
     3481 ggttgcgcgg ctcaaagccg ccggacttca gtctgtgccc ggtgccggcg cagagatcct
     3541 tgtagatcgt gttcgctcgg aaatttcacg cggaaaatgc gacacccaag agtggctgga
     3601 tgcccacagt atatttcaca aacacggctt gacatccacg gcgacgatga tgtacggtca
     3661 tgtggaaacc cgcgaagaac gcatcgaaca tattctaaaa atccgcgatc ttcaggataa
     3721 cagtttgaaa aactacggtc tcggtttcac cgcatttatc ccgtggaatt tccagccgga
     3781 taatgtgcct ctcggcaaaa accaaaacct acgtaccacc acgacagtag agtatctcca
     3841 gacattagcc atttctcgcc ttttgcttga taatattgat aattttcagg tgtcttgggt
     3901 tacacaaggg caagacatcg gccaattggg tctcaattat ggcgccaacg atttcggcag
     3961 tctcatgatc gaagaaaatg ttgtaagcca aaacggtacc tcttttaacg taacggtaaa
     4021 agacatagaa aaaatgattc gtgatgccgg ttatgaaccc aaacgccgaa ataatcaata
     4081 cacttttgtt aattgattga gcatagcctc ttcgcaatag catgtcacgt aacaaacgga
     4141 tcgcttttga tttgcaaatt aatttaaaac cggattcacg gtttatttcc tttctatgac
     4201 aaaactctca tccaaagatg tcctccgcat attagactta caaccgcacc ccgaaggcgg
     4261 cgcttatcgc gaaacttacc gttcaaaaaa tccggcaaaa gtggatccgc aacgatcggt
     4321 atgcaccgcg atttattttt tagtacaagc acatcaaaaa acagaatggc atcgtgtcaa
     4381 aaatgacgag atgtatcatc attacgccgg cgcaacactg gacgtcctcc tcgttagccc
     4441 cgacggtgtt tttgaaaaac ataaacttgg cgatgattta gagacaggtg cgcgaccgca
     4501 acttttaatc cccggtggtt attggcaatc ggtacaagcg catgattctt gggttttggt
     4561 aggatgtacg gtctctccgg gattcgattt ttcagatttc gaattgtcca acgaagccgc
     4621 tttaaaaaag caattccctc acctatcctt ttgaaaggta cacgtatgac cacgcaggaa
     4681 gtttttatca acaaaaatta tcagatgcgt gcaggatggc gcgtacttat tttcattgtc
     4741 gcatttatcc tgatcagcat gccatccgtt tatctgggat tattatttac taagtttctt
     4801 gagttcccca aagaatacaa tgaaattatc gtaaaatttt taaccactat tatcccttta
     4861 ggtttatcat ttttcctctt gaaaaaactc gataaccgct ctaccgatta tcttggatta
     4921 aaaacggact gtgccggatt tatagagttg gcgtggggac tccttatcgg ttttgtaatg
     4981 ttgacgatag cggttctaat accgtacgcg atgggctatg tgtcgcttca gttcgcgacg
     5041 gaaccgcctt cgtacttctt tagcggtctg gcattcaatc ttttactttt catcgtcgta
     5101 ggttttaacg aagaaatttt attccgcggt tatatctttc aggctaccgg tgagggactt
     5161 gggttcgtcg gcgccacgct gctttattct ctgctttttg gggctgctca tctcggaaat
     5221 ccgaatgttt ctctatttgg cattattaat atcgtgttgg ccggagtctt attatcgctc
     5281 gcgtatatca aaacacgcgc gctttggatg ccgatcggca ttcacattgc gtggaatttt
     5341 acgcagggat atatttgggg attaccggtg agcgggacga cggtcattca accaatgatc
     5401 atcgctcagg agatcggacc ggacgaaatc accggcggaa catttggtcc ggaaggcggt
     5461 gcaatgtgct cgatcgtatg catcggcgct tgtttattga tatggaaata tttttcccca
     5521 tccgatgcga tgaagcaggt catacaagag gccgagaccg ctcaaccatt caaacccaat
     5581 atcagcgaat caatcggcag taatactaac ccgaataacg ggatttcata aatattttca
     5641 agaaaactat ccccaaaaac cgcagatggg gcgactcacc accgtattgg cgttagattt
     5701 atagggaatt ttttgtttta ccgttaaaaa caaaatgtac ccataaacta acttaaagaa
     5761 aggtggatat gaatcggaat atcttttttt tgggagtgtt atttttttcg tcttttcttt
     5821 ttgcacatga cggagatata cacaaagatt cttccagtac gaccgttcgt acgataacca
     5881 atattgctcc caatatctac atgatacgcc atccggacgc cccggacggg tttcctcaag
     5941 gcaataccac ggttatcatc ggtgatcgcg aagtattggt agtggatgcc tgctacttgc
     6001 cttcttcagc caaacaagat atcgaacaaa ttcaaaaatg gacttccaaa ccggtacgtt
     6061 atctcgttaa tacacactgg cactacgatc acacaatggg caacgggaca tacgccgagg
     6121 cgtttccggg aatttccatc atcgcgcatc acgagaccaa aagaatcatt cagggttata
     6181 acccgggttg gtttgcccgt tttcccaatc gttcagcgat ttttaaaagt gccatcgaat
     6241 ccggaaaaga cgccaatggt aacaaattat ccgaaagtga tatacgcgac tacaaaaagt
     6301 atcttgacgg attgacaccg gtacaagagg aatttagtaa aatcaaagat cgcgcaccca
     6361 atctttcgtt tgatcatgaa ttgacaatag atctcggtaa tcgtgaagtg caaataaaac
     6421 acctggggcg tggaaattca gccggcgata taatccttta tttacccaaa gaaaaaatca
     6481 tcgtcatcgg cgatttgatg gatcaccctg taccatatct tggcagcggt ttccccggtg
     6541 aacaagtcga aacactcaac aaaatagcgc agcttgatgc agatacattc ataccgggcc
     6601 acggaaatgt tctcaccgga aaatcgtatc ttttcttatt acgagacttc ctcgccgaag
     6661 tcgtagcggc ggtgcgttct gaaatcgaaa aagtaggaaa taatccccga ttgattgatc
     6721 aggttcgaga gtcggttcac aaacgggttg attttacggc atggaaaaag agatttgtcg
     6781 gtgacaataa agaagacggc gatttttttg acagcttctc tgttcaaggc gtttttaact
     6841 gcgctttcaa tgagcttgta agaaaataat ttttcgcaat agtcttataa accggcttat
     6901 tcatactccg tagaggttat gcggttaaga taataaaatc tggacatttc atgttcatgg
     6961 ttatctgaca gtctttagca taacctctat tgttataaaa ttcttccctc actatctacc
     7021 acgcatattc ataattgagc cgtcatttta agggtcgcac ccttagttat ggtgatttaa
     7081 tagataccga aatcacgtaa tttacgcaaa ctattgtaaa atcaacactt tttcaattga
     7141 cttagctata tttttttgtt atattcgcgg gttgcgctaa gaggttgatt tctattattt
     7201 tcaataattt agcgcgttgc aacaaactat tcatcgtgtt tgaggctttc atgaaacttg
     7261 cagattttaa gtttaagtta ccggaaaaat acatcgcgca gtacccggtc gaaccgcggg
     7321 acaaagccaa gcttatggtt ttggatcgta aaacgggtga tatcgaagat aaaatatttc
     7381 gcgatattac ggaacacatc acaaaaaacg attgcctcgt cctcaacgaa actcgtgtat
     7441 ttcctgcccg tttgatggct cgcaaagaaa aaaccaacgc taaagtcgaa gtgtttttgc
     7501 tccgcgaact ggaaaacaat ctgtgggaag tgcttgtcaa acccgcacgc aaagtgcgta
     7561 tcggcaatcg tcttgtagtt gatggcgtct attgcgatgt tattgataat acggtttccg
     7621 gaggccgtgt tgttcgcttc atttaccctc aagccgattt ccataaattt atagacaaag
     7681 taggccagtg ccctctgccg ccgtacatca aacgttctgc cgaagagagc gataaagaag
     7741 attatcaaac cgtatttgca cgtgtacgcg gctcggttgc agcacccacg gcaggtttgc
     7801 attttaccaa agaactgctc ggacgcctga aacgtaaggg tgttactatc gtaccgctcg
     7861 tgttgcacgt cggtctcggt actttccgta aagtgcaggt ggaagattta tcacggcaca
     7921 aaatggattc ggaatatttt gaaataccgg aaattaccgc gcgcgcgatc aatcaaacca
     7981 aagataaagg cggcaaagtg atcgccgtgg gttcgaccgt cgtacgcagc atcgaatccg
     8041 ccgttacctc gcttaaccgc gtaaaaccgc atcgcggttg gaccgataag tttatgtatc
     8101 cgccgtatga atttaaaatc gttgataaaa tggttaccaa ttttcataca ccggaatcca
     8161 cacttttgat gctggccgca gcatttggcg ggaaagataa catcttcaag gcctacaaaa
     8221 aagcgatgaa agataattat cgtttctaca gctttggcga tgcaatgctg atcgaataaa
     8281 aaaactacat gaagataaaa aacccgcttt tgagcgggtt tttttattta gccaaaaata
     8341 tactaaaaca tcaaacggca acaacgtact ttgcaaagtt atcgagaatc gcttgccatc
     8401 cgtttttttg catatcaacg ggattctctt gttccgcatc aaaaacgcta acaattttcg
     8461 tgctattgcc tgattgagaa aaatgcacgc gcaccttcct tccgtcttcc atcgtataag
     8521 cgatcaattc atactgtacc acttcatcgt acgagcctgt gaaatcaaag ccgaaactgc
     8581 cgtctttggc ttccatacgc gataaaaaac gcccgccgac acgtaaatcg ttttctgctt
     8641 ttgttgtatg ccagtcgtcc gaggcggcat tccacttaac gatatgttca ggcgatgtcc
     8701 acactgacca aactttttct agcgaggctt gtatcgtagc ctctatggtg acttgtgtgt
     8761 aaggattttg catgaatgtg atctttcaaa aagacgttgt ttatctcagt taaaacatct
     8821 cagtttgata aaaagtaact gcgcttttac ttcttcaaaa gtttaaaggc atgcatccat
     8881 gaattggatg taaaacccgg aatacaccat aatatacaga gcggttctat cgcacgggcg
     8941 gcttcgactt tgcccatgtc cgcccaatcc caaccaaatt gcgctaaaat agccgtgact
     9001 tgtgccttgg cggcatcgtt attaccgcaa atgaacatgg taggcgtacc ttccgccatt
     9061 ttgggattga ccattagtgc attaccaaca caactaaaag ctttgacaaa attggcctcc
     9121 ggtgcaagaa cctgtaattg ctccatcaaa gattcattgg gtccggtaaa aaattgcaat
     9181 acgccgttga ccggaggaac ttctttgatc gggttattta catcaatcac agttttgttt
     9241 tttagattgg tgatgccggc gcttttgact gcatcttcag ccgcatgacc ttttacggca
     9301 agcacgatca gctcaccaaa tgatgcggcc gtagccatac tccccacctg agctttatcg
     9361 ccgtttttgg atttccattc ggataattta gttacgtcac gcgttcccat catcacttca
     9421 tgaccatgct tgataaaacc actggctaat acctgagcca cttgaccgga gccgagtaca
     9481 cctattttca tattaatttc tcctgttgtt aataaacttt taatgtcgtt tttcatcaca
     9541 atccgcaatt tgaattataa tcaataacgc gaaagcgttt tttaaattaa ttttttcgta
     9601 cagagtttac ttccatcata ttttttaccg tcatatcaga tttattttct ccgatttgtt
     9661 gacgccacat ggcgtaatac agtcccttct gtgcaagcaa ttcgtcatgt ttcccggctt
     9721 cccctatgcg acctttttct aatacgaata tccggtcggc atgcatgatg gtcgaaaggc
     9781 ggtgcgcaat acatatcgtc atatgttcct gacggtgtga gatatcacgg atcgtatgcg
     9841 tgatttcttc ttctgtcagc gaatccagac tggatgtggc ttcatcaaaa accaacaaac
     9901 gcgggcgtcg taacagcgcg cgggcaatag acaagcgttg tttttcgcca ccagaaactt
     9961 taacgccgcc ttcgccgatc accgtatcga ggcctttatc agcacgcgcc agcaaactgc
    10021 cgcatgcagc tttattcaaa acatcgagac attcctgatc cgttgccttc gggttgacaa
    10081 acaataagtt ctcccgtatc gtacccgcaa aaagctgtgt atcttgcgta acaaagccga
    10141 tctgctcgcg tagcacatcc aaatcgatcg tgtcgccgcc atggccgtta tatcgtacga
    10201 taccggactg cggtttgtat aaaccgacaa gaagtttgac cagcgtagtt ttaccggcgc
    10261 ccgacggacc gacaaatgcg atcgtctcgc cgcgatgcac cgaaaatgaa attccgtcca
    10321 aagcatggcg ctgcgccgac tgatgtttga acactacatt ttcaaattcc aatttttcta
    10381 tatcggcgat cagtacggga ttggccggtt tggcttcgca cggtgtgttg agaatatctt
    10441 caaaattttt gagcgacacc tcggcttcgc gataaatatt gataacgttg cctaactcct
    10501 gtagcgggcc aaatatgaaa aaggaataaa tgaacaacga aaagaattca ccaaatgtaa
    10561 tctcttgggc gaagatcaaa tacaacatga taaataaaat cgaatttcta agaaaattaa
    10621 ccagggtccc ttgtacgaaa ctcaagctcc gtatgtactt cacttttttg agttccagct
    10681 tcagtatctt tttggttgta ccgttgagac gttcgatttc ctgctgtgcc aatcccaaac
    10741 ttttgaccaa ttcgatattg cgcaaggatt ccgtcgttga gcccgccagt gccgtggttt
    10801 ctgcgacaat ggctttttgc accactttga tttttttgct gagcaccgaa ctcacccagc
    10861 cgataatagg tatggtagaa aaataaatcg gagcgataac ccaatgcacg ctgatcgcgt
    10921 aggccgtcac gaaaataatt ccgaccaatg tcgtaaataa aatattgata aatgccgaga
    10981 tcaggcgttc gacatcggtt ctgacttttt gaagtttacc cagtgtttct ccgctgcgct
    11041 gatcttcaaa cgtgctataa ggaagctcca gtgaatgccg cagaccgtcg gagtatattt
    11101 gggcgcctaa tttttgggtg atcgtattga tataatagtc ctgaaaattt ttggcaacac
    11161 gcgacacaaa agcgacgccg atcgctgcgc cgagtaatac agatacgcct ttgacaaact
    11221 catcaaacga ataatctttg tagcgcgtcg catattcgtc gatcacgtga cgaaaaatcc
    11281 acggatcaag caatgaaaaa atctgattga ccgccgccaa aaacaacgcc aaagcaacca
    11341 atttccaata atttttcaga taccctatca acaatttcat aatctcatac tttctattaa
    11401 ctgacttagc aaaattattc tgtaatcatt tcgaccagac gacgtatgcg cggaacagct
    11461 atgaacgcga cgggaaaagc aactaaaaac gaaaaacccc aggcctgaag ccataaagcg
    11521 acatgccacg tctccatacc aaatgtgcga aatgtcatca ccagggacat aatgcaactc
    11581 aacgtcagcg ccataaagag gacgaataac acttgggaat gttttttgtt aaatttcata
    11641 cacagaatcc gttttaattc atcgaaaata aggtcgaacg tactgtagct ttattgtgaa
    11701 aggctaattc ataaatatcg aagcgaaaat aatcgtacag tggaaacgta gctaatatgt
    11761 caaacgcttc cgaatcactt tgtgcccgga tgacaatcca gagtttggta cgatcggcag
    11821 acaaagcata actttgtaca acccgttcct ccaatagccg attgatcaat gtgcgctgtt
    11881 ccggaatttt ggagaaaaac gcttcatcgg gaaattccgg taaagataca tccaccatat
    11941 attgattcac tttacgcctc ctgcattatt tttaaaaccg cgccgcatta tccttggcaa
    12001 atcgttcaaa atctatcgga tctttcccgg tcatatcacg cgtcacgttt gaaatttctg
    12061 cggcgtaccc cgctttgttg atcgcatgaa gctctaataa cccgtcaatg gcccagtttg
    12121 gcatttgcat tt
//
LOCUS       DAOQNQ010000067        12101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1861_length_12101_cov_4.953429, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000067 DAOQNQ010000000
VERSION     DAOQNQ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12101
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1861_length_12101_cov_4.953429"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PK754_06150"
     CDS             <1..540
                     /locus_tag="PK754_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09118.1"
                     /translation="SLYKMIFPEDTTTTESSSDYNMPSRGIDALFMPIYDDEIKYIAP
                     QIAYYNIQTKWLGGDHWNALDELRRQNKYVNGCIFVGEFFSNPDAGPTKQFIKNYTAA
                     AKKRPTPEAFYGYDIVGVLGVIIERGAQLPDEVQNELQNGITYEGVHNQIRFDKGRRV
                     NASVHLLQFKDNTIHKIEP"
     gene            561..7265
                     /locus_tag="PK754_06155"
     CDS             561..7265
                     /locus_tag="PK754_06155"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNB09119.1"
                     /translation="MRQIRFFILLIFTSIPLAFAQSDSITISEVMFSTSNTLDFIELY
                     NYSKTQTIDLAKYIITDGAGNNDSDRISSAGEGLLLYPGQFAILLDPDYFLPPNASGR
                     VYDISIPDTARIVTIDTNQTGGFVLGNGSFSNTTAETIHLIKVQSGIPRDTISRYTYS
                     LGNNTSYSDEKITLNNDNNSSNWTNSIYLNGTPGAPPETNLSINDHRMIFTPPDPLTG
                     ASVTIGAIVTNKAIATSDSFRVLFYHDADKNGTADVSELLDSVSYNVSLPRNTSDTIF
                     FTAPSIASGINYYIARIGSIYPYADTVLSDQSLRDSVQTTPAYNVSWSASSVFSPSSP
                     ATGDTVTYARAIYNAGIADITHFAAYLYIDQNHNGMIETDEKVDSANYNDSLPSGDSV
                     VIYFTIPDVQRGKLHLLLDLPSDSLGPLPDEQPADNRTSDSANIRAAMSLTLTSASAL
                     KPSYNYIADTMAIKAVVHNNGSTSASGFSLILYHDANNNGLPDTNERVDSVRYTSSLD
                     AAASDSITLHWLPTSEGNISLIVKLDSTTVQPLTDEDGSDNTHTLNALIYPRPDSITF
                     SEIMFDPLGSEDSNEFIELYNFGTHAVDLLNWKIADSLGYDAIQDAGNGTVLQAGQYA
                     IVFENEYTLNTGRYTSRIPSSALVLTINGSTFGSSSSGLVGTYPKQLFLVRSLGDTIS
                     TYRYSLDNTEGHSDTKINPSAANASVNWMNSLITDGTPGAHAEVNLSVLGQRLRFAPS
                     EIPSGTQTVISVTVKDSGIAPVNIFKIAMYHDTNRNATPEPEEIIDSLVFSGQLYYGD
                     SVIFTANTPTLSYGVHYFIARISQGEVSPYGDTLYYNDMLTDSIRTQPSGDIALNRGR
                     LILGEIATRGNSFPIGAYAYNMGTQPVQSFTVRWYEDRDGNYTTAPEERIDSSTYAGN
                     LLPGDSTFLQINYPNATYGKHRIMVEVISTSVQPIVDEEPSNNLLVDSLEAGARARDI
                     LISEFMYLAVTDSIPEWIEIYNPSALSISLMNWQLYDKTSHVVLTERSDTLHTGQFGI
                     IVKDSTLFHKKYGEALSDVLYIYPTSMPSLNNTGGDIIKLSTPAGLTVDSLNYLTSWG
                     DDEGISLERKLNDSASYSMSNWALSSDPSGGTPGTKNTATPQLHDLSITSRDILWTPA
                     YPTQGEVFQIKATVRNLGVTSSGASAILLYAYSGADSTLLQTTNSPAILPGDSLVIVS
                     SPITNGPQTYVGIQIDYAEDERLSNNFAKRFVPAATPRQTVIINEIMYKPGTGHTEWI
                     ELYNRSQNAVDLRSWRISDGNADLTAPATAKTITVQSVILQPDSFIIIAKDSVQFSSQ
                     YGTPLHAVYLSSMPALNDPGDMITIQDSLGSIVDSLVYLSGWGGSSDRTLERRFYDSV
                     SYLTSNWLSCESPIGATPGHPNSVKPLHYDISLNASPLHVTPSYPRIGEPFTITAPIY
                     NQGLEPVNDASLRWLRIRPQHDTLILSEQIIGMLNAGDSLKTSYSDTARQIGDSIVAL
                     ILSVSDERTLNNKSAVPLITSARRNALVINEIMYDPGSNRPEWIELYNPGADTVDLKN
                     WRLADESTIANPKTFAGTSLRIPSQHYVIITKDASALALSYPLLSSPVVTMTSFPTLN
                     QTGDLILLTDSLSVTVDSVFYENTWGGANGFSIERISATDDANSPNNWKTSEANAGAT
                     PGGMNSTTPVALDLSIRTNDVWFDPPFPSPGQPVQIHMRIRNKGLQNITNAAVVRVYN
                     DINNDSIPQITEIIDSTTVPVLLAGDSQSVTIQWTIPASASRRLSKAVSMGSRKIIIQ
                     LDFASDERTENNLLFTALPLGTPFQSIVINEFMYETDTSRTEFIELYNPGTTAWDLKD
                     WKIADGSSVVSIADVSVSFLSGAYKVLAPDSSFFRKFSTTPASSVIIVPGLPSLNNTG
                     DAIRLADDLGATVDSLTYSNTWGGSKNISVERIDADVSSDEPNNWTTSLTAQGHTAGS
                     VNSIVSAEPFVKQSLIINEIMFSPLSGEPEYIELYNRSETAISLLNWAITVGDDKTLL
                     AQTDISVPPHAYVVIAGTKTFPDRYDLDKTQIIVPLTNLAALPANGTALVLRDWVGTT
                     IDSLAYTELWGGGDGYALERIQPDAASDLMTSWRTCVFVEGGTPGKINSVYNGDKPKK
                     IRITADPNPFLTDVAENTTITLEMPVTYARVTMKIYDSQGRLMITLLNNQPTGAHREV
                     IWDGRDKNGQRVRMGIYIIFIEAIEESGGFNKTAKQTIVVGKRL"
     gene            complement(7348..8142)
                     /locus_tag="PK754_06160"
     CDS             complement(7348..8142)
                     /locus_tag="PK754_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNB09120.1"
                     /translation="MIKRLLFILVGVQSLWAQTEYPEAISDNSFMLEEAYNQEARVVQ
                     HISGLIYTRPSKDWEYGLTQEWPMFFLPHQEKHQLSYTLPYQWLHSGPSGFGDVAVNY
                     RYQWMADPAGYSVSPRFTVLLPTGDDNKGLGSGKMGYQIGLPVSKRFSAQWAAHVNVG
                     MTMVPGVDVGSKKKIQKAYNIGASAIWLVKSNLNVFVEYVSVFEDEVDGTSLKNKGTH
                     TINPGFCFAKEIGSMQVVPGLSVPLQLNGSSKTKQVFVYLSMEHPF"
     gene            complement(8156..8860)
                     /locus_tag="PK754_06165"
     CDS             complement(8156..8860)
                     /locus_tag="PK754_06165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB09121.1"
                     /translation="MKTALITGASSGIGLATAQKLTEMGYRVYGLARNFDKTDWRHAN
                     FVPIVCDVRDIKALEEVEKKWPTEESLDVLIHSAGFGYFAPHEEIPLRTLQDMVTTNL
                     TAPLILTHLFLRRIREQRGFVIHIASVTALEPSRMGAAYAATKAGLRHFSASLFDECR
                     KQGVKVVNINPDITRTAFYDNLNFSPSDETDTYIEPQEVADTVAHVLTQRDGTVISEI
                     TIRPQRLHVIKRKHHH"
     gene            complement(8857..9876)
                     /locus_tag="PK754_06170"
     CDS             complement(8857..9876)
                     /locus_tag="PK754_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09122.1"
                     /translation="MHAWSGFDKSSFSKHFSRIYIEDGAEAYPDAQTILARFPTARRI
                     PIRHYKDVFNRKHQDFQLQKRAMDIILAVKREGYLYAGSPQTPSFGHDRFFYNAMVLN
                     CVYNCDYCYLQGMFPSAHLLFFVNPDAFFDSVVAELAKGPLYLCVSYDTDLLAMESFL
                     PQCHRWIHFAAQRRDLVVEIRTKSGAYDAIRDTPVSDNVILAWTCSPDEIIKVYESGT
                     PPLDTRLSAMAQAVKDGRKVRLCIDPILKIKDWQAIYSRCIEYIFERIPGAALHDVSL
                     GVFRMNKNYLNNIRAMRVDSDILFYPYDEKDQTMSYTDLDRESMLDYIRKTMSAYVQE
                     ERIIL"
     gene            complement(9836..10669)
                     /locus_tag="PK754_06175"
     CDS             complement(9836..10669)
                     /locus_tag="PK754_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09123.1"
                     /translation="MKKNTKHGIVVALRPEAAPLIRAWRLKKNIAHTAFEVFENETIS
                     LIVSGIGKIKSATAATYLYTTGHLHTLWNFGICGASDTTLDIGSFWRIHKITDVVSGR
                     DYYPDGSIRLTMGESVLRTVDRPQENQCVGSADHALYDMEASGFFVASTRFLPLERIH
                     VLKIVSDHLQIKGMTSDFIENLTMQCLPKFSSCLDVLSAMRIDKETLSVNEQETMQHI
                     FEMINPSHAQRIALFDAARSYKIRNGYLPGDLMDMTQWAGENKQERNARVERLRQKLI
                     Q"
     gene            complement(10653..11063)
                     /gene="mce"
                     /locus_tag="PK754_06180"
     CDS             complement(10653..11063)
                     /gene="mce"
                     /locus_tag="PK754_06180"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNB09124.1"
                     /translation="MKLEKIDHLGIAVPDLEKFIELYKAMGFEYHGREIVADQKVETA
                     FFKVGESSIELLAGTDPESPITKFIEKNNGKGGIAHVAIGVTGIEEKLADLKAKGFQL
                     IDEVPKTGAHGARIAFVHPKSTGGVLLELCEKKH"
     gene            complement(11069..11944)
                     /locus_tag="PK754_06185"
     CDS             complement(11069..11944)
                     /locus_tag="PK754_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HNB09125.1"
                     /translation="MNAKIIDGKQIAESIKREIAQEVEALKIKNVQPKLVVVLVGEDP
                     ASQVYVRNKGLACEQVGMAHETIKQPATMPEKDLLALIDQLNKDPSVTGILVQLPLPK
                     HINETLIINAINPAKDVDCFHPFNVGRLAIGDAIFLPCTPAGVQELLMRSEADPSGKH
                     TVIVGRSNIVGKPLANMLVQKAKGANSTVTVCHTGTPDMTYFIKQADIVVAAVGRAEM
                     IRGEMLKPGAVVIDVGINRISDPSTKSGSRLVGDVHFDSAKEVASAITPVPGGVGPMT
                     IVMLLKNTLKAAKQG"
BASE COUNT         3251 a         3054 c         2597 g         3199 t
ORIGIN      
        1 tctctttaca agatgatatt tcccgaggac accacgacga ccgaatcctc cagtgattat
       61 aatatgcctt cgcgcggaat tgacgcgtta tttatgccta tctatgacga cgaaataaaa
      121 tatatagccc ctcaaatcgc gtattataat atccaaacaa aatggctcgg gggtgatcac
      181 tggaatgctc ttgatgaact gcgtcggcaa aataaatacg tcaacggatg tattttcgtc
      241 ggagagtttt tctccaaccc tgatgccgga ccgaccaaac agtttatcaa aaactacacc
      301 gcagcggcga aaaaacgccc tacaccggaa gcattctacg gatatgatat cgtcggtgtt
      361 ttaggggtga ttatcgaaag aggcgctcaa ctgcccgatg aagtacaaaa cgaactgcaa
      421 aacggtatca catacgaagg cgttcataat caaatacgtt ttgacaaagg tcggcgcgtc
      481 aatgcatccg tccatctttt gcagttcaaa gataatacca ttcataaaat cgaaccgtaa
      541 cccgccgccg gaaaccacgt atgcgacaaa tacgattctt tattctgctg atatttacct
      601 ccataccgct tgccttcgct cagagtgaca gtatcacaat atcggaagtg atgtttagta
      661 caagtaatac attggacttc attgaacttt ataactacag taagacacaa actattgatc
      721 tcgctaaata cataattacg gatggtgcag gcaataatga ctccgatcgg atcagtagtg
      781 ccggtgaggg cttactttta tatccaggcc aatttgccat tctccttgac cccgattact
      841 ttttaccgcc caacgcttcc ggacgtgttt acgatatttc cattccggat actgcacgca
      901 tcgtcacaat tgataccaac caaaccggcg gctttgttct cggaaacgga tcgtttagta
      961 atacaaccgc agaaacaatt cacttaatca aggtacaatc cggaattccg cgtgacacaa
     1021 taagtcgtta tacctattca ttaggcaaca atacttcata cagcgatgaa aaaatcacct
     1081 taaataatga taacaattca tccaattgga cgaattcgat ttacctgaac ggtacgccgg
     1141 gtgcaccgcc ggagacgaat ctttcgatca acgatcatcg catgatcttc acaccacctg
     1201 atccgctcac cggagcaagc gtgactatcg gcgccatcgt aaccaataaa gccattgcca
     1261 cgagcgattc atttcgtgtg ctcttttatc acgacgccga taaaaacgga acagcggatg
     1321 tttccgaact tctggattcg gtttcctata acgtgtcact accccgcaat acatccgaca
     1381 cgattttttt tacagcgcct tcgatagcct ccggcattaa ttattatata gcccgtatcg
     1441 gttcgattta tccttatgcc gataccgttt tatccgatca atccctacgc gattccgtac
     1501 aaacgactcc ggcctataat gtatcatggt ccgcttcttc tgttttttca ccgagttcac
     1561 cggccaccgg tgatactgtc acctatgcgc gtgcgattta caatgcaggt atcgccgaca
     1621 tcacccactt tgccgcctat ttgtacatag atcaaaacca taacggcatg atcgaaaccg
     1681 atgaaaaggt cgattcagca aattataacg attcccttcc aagcggtgat tcggtggtta
     1741 tttattttac gatcccggac gtccaacgcg gaaaactgca tctattatta gacttaccgt
     1801 ccgattcttt aggtcctttg ccggacgaac aaccggccga taatcggaca tccgattctg
     1861 ccaatatccg tgcggccatg agcttaaccc tcacatcggc cagtgcactt aagccgtcgt
     1921 ataattacat cgccgatacc atggcgataa aagccgtagt acacaataac ggaagcacat
     1981 ccgcgagcgg tttttctttg atattgtatc acgacgccaa caacaacggg ttgcccgaca
     2041 cgaacgaacg tgtggattct gtgcgatata catcgtcatt ggatgcggcg gctagtgatt
     2101 ccatcacttt gcattggcta ccgacgtctg aaggcaacat ctcgctcatc gttaaactcg
     2161 attcgaccac agtccaacct ctgacggatg aagatggatc agataataca catacgctga
     2221 atgcgctcat ctatcctcga ccggattcga tcactttttc agaaatcatg tttgatccgc
     2281 tgggttccga agattctaat gaatttattg aattgtataa tttcggtaca cacgctgttg
     2341 atctgctcaa ttggaaaatc gctgatagcc tgggatatga tgcgatacaa gatgcgggaa
     2401 atgggacggt tttacaagcg gggcaatatg ccatcgtatt cgaaaatgaa tatacactta
     2461 atacgggtcg ttatacatcg cgtatcccat cgtcggcatt agttttaacg atcaacggct
     2521 ccacctttgg tagcagttca agcggattgg tgggaaccta tccgaagcag ttatttctgg
     2581 tacgcagttt aggtgatacg atctcaacgt accgctattc tctcgacaat accgaaggac
     2641 attccgatac aaaaatcaat ccctcggcgg ccaacgcgtc cgtgaattgg atgaacagtc
     2701 tcatcaccga tggcacgccg ggcgctcatg cggaagtcaa cttgtcagtc ctcggtcagc
     2761 gtttgcgatt tgcgccttcc gaaattccat ccggtactca aacagtgata tccgtgactg
     2821 ttaaagattc cggcatcgca ccggtgaata ttttcaaaat cgctatgtat catgatacca
     2881 accgaaacgc cactcctgaa ccggaagaaa tcattgattc cctggtgttt tccgggcaat
     2941 tgtattacgg tgacagtgtg atatttacgg caaatacacc gactctctcc tatggcgtac
     3001 attattttat tgcacgtata tcgcaaggcg aggtctcgcc gtacggtgat acactctact
     3061 ataacgatat gctcaccgac agtataagga cccaaccatc cggggacatc gcactgaacc
     3121 gcggacgcct gatattgggt gaaattgcga cgcgcggtaa ttcctttccg atcggtgcgt
     3181 atgcgtacaa tatgggcacg caacctgtcc aatcgtttac tgttcgatgg tatgaagatc
     3241 gcgacggaaa ttatactacc gcaccggagg aacgaataga ctccagcaca tatgccggaa
     3301 atcttttacc cggagattca acctttttac agatcaacta tcccaatgcg acctacggta
     3361 aacatcgaat tatggttgaa gttatttcga cctccgtgca acctattgtc gacgaagagc
     3421 cgtcaaacaa tctgcttgtg gattcgcttg aagccggagc acgcgcacgc gatattctga
     3481 tcagcgaatt tatgtatctt gccgtaacgg atagtattcc cgagtggatc gaaatctaca
     3541 acccttctgc gctgtccata tcactgatga attggcaact gtacgacaaa acatctcatg
     3601 tcgtattgac ggagcgaagt gacactttgc acacaggaca atttggaatc atcgtcaaag
     3661 actccacttt gtttcacaaa aaatacggtg aagcattatc ggacgtttta tatatctatc
     3721 cgacctccat gccgagcctt aataataccg gcggcgatat tataaaacta agtacacccg
     3781 cgggacttac cgtggacagt cttaactatt tgacttcatg gggcgacgat gaaggtatta
     3841 gcctcgaacg taaactaaac gatagcgcat cgtacagcat gagcaactgg gcgctcagca
     3901 gcgacccttc cggcggtacg ccgggtacaa aaaacacagc tacacctcag cttcacgatc
     3961 tgtccatcac ttcacgtgac attctatgga cacccgctta tccgacgcaa ggagaagtct
     4021 ttcaaatcaa agctactgta cgaaatctag gcgttacctc aagcggggca tccgctattt
     4081 tactctatgc ctacagcggt gcagattcta cactattaca gacaacgaat tcacctgcca
     4141 tattacccgg tgattctttg gtgatcgtct catccccgat taccaacgga cctcaaacct
     4201 atgtcggcat tcaaattgat tacgcggaag atgaacgtct atccaataac tttgctaaac
     4261 gtttcgtacc tgcggccaca ccaagacaaa cggtgatcat caatgaaatc atgtacaaac
     4321 ccggaaccgg acatacggaa tggatcgaac tttacaaccg ctcacaaaac gcagtggatc
     4381 tgcgttcatg gcgtatatcc gatggtaatg ccgacctgac cgcaccggct actgctaaaa
     4441 ctataaccgt tcaaagtgtc atacttcaac cggatagttt tatcatcatc gccaaagatt
     4501 ccgttcaatt ttcatcgcag tatggaacac ctttgcacgc cgtttatctg agcagtatgc
     4561 cggcactcaa tgatccgggc gatatgatca ccatacaaga cagcttaggt agtatcgttg
     4621 atagtctggt gtatctatcc ggttggggcg gcagttcgga tcgtacattg gaacgacgtt
     4681 tctatgattc cgtttcgtat ttaacttcca actggcttag ctgtgaaagc cctatcggcg
     4741 caactccggg tcaccccaac agcgtcaagc cattgcatta tgatatatct ttaaatgcat
     4801 ctcccttgca cgtaacgcct tcgtatccgc gtatcggcga accatttaca ataactgcgc
     4861 cgatatacaa tcagggcttg gaacccgtga atgatgcctc tctgcgatgg ttacgtattc
     4921 gcccacaaca tgataccttg attctatcag aacagatcat cggtatgttg aatgccgggg
     4981 attcattaaa aacttcttat tcggacacgg cacgccaaat cggtgattcc atcgttgcgc
     5041 ttatcctaag tgtgtcggac gaacgtactc tcaataataa atccgctgtt cctctgataa
     5101 cctccgctcg tcgtaatgca ctggtgatca atgaaatcat gtacgatccc ggctcgaaca
     5161 gacccgaatg gatcgaactg tataatccgg gcgcggatac tgttgatctt aaaaactggc
     5221 gtctggcgga cgaatccacc atcgcaaatc ctaaaacttt tgccggtacg tccttacgta
     5281 ttccgtcaca acattacgtc atcattacca aagatgcatc cgcacttgcg ctttcttatc
     5341 cgttactatc atcgcccgtc gtgaccatga cctcttttcc aactctcaat caaaccggtg
     5401 atttgatact gctgacggat agtctgagcg taacggttga cagtgtattt tacgaaaaca
     5461 cgtggggcgg tgcgaacggt ttttcgatcg agcgtatcag cgcaaccgat gacgccaatt
     5521 caccgaataa ctggaaaacc tctgaagcca atgccggcgc cacaccgggg ggaatgaata
     5581 gtacaacacc ggtggctctg gatctcagca ttcgaacgaa tgatgtatgg tttgatccgc
     5641 cgtttccttc accgggccaa cccgtacaga tccatatgcg tattcgcaat aaaggcctgc
     5701 aaaacatcac aaacgcagcg gtagtgcgcg tctataacga tatcaataat gatagtattc
     5761 ctcagataac ggagatcatt gattctacga ccgtaccggt gttattagcc ggcgactcgc
     5821 agtccgttac catccaatgg acaatccccg cttcagcatc tcgtcgttta tccaaagccg
     5881 tatctatggg atcccgcaaa atcattatcc agcttgattt tgctagcgat gagcgcactg
     5941 aaaataacct cctatttact gcattgccgt tgggtacgcc gtttcagtct atcgtgatca
     6001 atgagtttat gtatgagacg gatacttcgc gtacagaatt tatcgaactg tataatcccg
     6061 gtactaccgc atgggacttg aaagattgga aaatcgccga cggtagcagc gtcgtatcta
     6121 tcgccgacgt ttcggtttca tttttatcag gcgcctataa agttttggcg ccggacagtt
     6181 cattcttccg taaattttct actaccccgg cctcgtccgt aatcatcgtg ccgggtttac
     6241 caagcctcaa taatacgggt gatgccatcc gtctagccga tgacctcggc gcaacggtgg
     6301 atagcctgac gtatagtaac acatggggcg gctctaaaaa tatcagcgtt gagcgtatcg
     6361 acgccgacgt ctcatcggat gaaccaaaca attggacaac gtccttaacg gcgcaaggac
     6421 acacggccgg tagtgtcaat agcattgtat ctgctgaacc ttttgtcaaa caatcgctca
     6481 tcatcaatga aatcatgttt agccctttga gcggcgagcc ggagtatatc gaactataca
     6541 accgtagcga aaccgccatt tccctgctca attgggccat cacggtcggt gacgataaaa
     6601 cacttctcgc gcaaaccgat atatccgttc ctccgcatgc atacgtcgta atcgccggta
     6661 ccaaaacatt tccagatcgt tacgatttgg ataagacaca gattatcgtt ccgttaacca
     6721 accttgccgc actaccggct aatggtacgg cgctggtgtt gcgggattgg gtgggcacta
     6781 cgattgacag cttagcttat acggaacttt ggggtggcgg agacggttac gctttggaac
     6841 gaatccaacc ggacgccgca tccgatctga tgacctcatg gcgaacatgc gtattcgtcg
     6901 aaggcggcac gccgggaaaa atcaactcgg tatacaacgg cgataagcct aagaaaattc
     6961 gcatcaccgc cgatccgaat ccttttttga cagatgtcgc ggagaacacc acaatcactc
     7021 tcgaaatgcc ggtaacttat gcgcgggtta cgatgaaaat atatgacagt cagggtcgtc
     7081 ttatgattac attactcaac aatcaaccga ccggagctca ccgcgaagtg atatgggacg
     7141 gacgagacaa aaacggccaa cgcgttcgta tgggaatata tattattttt atcgaggcta
     7201 tcgaagaatc cggcggattt aataaaacgg ctaaacaaac gatcgttgtc ggaaaacgat
     7261 tataaaaaaa ataccgctgc ctcagaaata aatatatacg gataaattcc tttttctcct
     7321 gagacagcgg ttcaaataaa tttcacatca gaacggatgt tccatagata aataaacaaa
     7381 gacctgcttg gttttagagg aaccgttgag ttgaagcggt actgataatc cgggcaccac
     7441 ttgcatcgaa ccgatttctt tggcaaaaca gaaacccgga ttgatggtat gtgttccttt
     7501 attcttcagc gacgtcccgt caacttcatc ttcaaataca ctcacgtatt ccacgaaaac
     7561 attcaggttg gattttacca accatatcgc gctggcgccg atgttatatg ctttttgaat
     7621 tttctttttg ctgcctacat ccacaccggg gaccatcgtc atacccacat tcacatgcgc
     7681 tgcccattgg gcggagaatc gtttactgac aggtagaccg atctgatagc ccattttgcc
     7741 ggagcctaag cctttattat catcaccggt cgggagcaga accgtaaagc gcggtgacac
     7801 actgtatccc gcaggatcgg ccatccactg atatcggtag ttaacagcta catcgccaaa
     7861 accgctcgga ccgctgtgaa gccactgata aggcaatgta tagctcaact gatgtttttc
     7921 ctgatgcggt aaaaaaaaca tcggccactc ctgcgtcaaa ccgtattccc aatctttcga
     7981 cggtcttgta tagataagac ccgaaatgtg ctgcacgacc cgcgcttcct gattgtaagc
     8041 ttcttccagc ataaacgaat tgtcactgat cgcttcggga tattcggttt gagcccagag
     8101 gctttgaaca ccgaccaaaa tgaagagtaa ccgcttaatc ataatgatct ccttgttaat
     8161 gatgatgttt acgtttgatg acatgaagtc tttgcggacg aatcgtaatt tcggaaatca
     8221 ctgtaccgtc gcgctgcgta agtacgtgcg caacggtatc cgctacctcc tgaggttcaa
     8281 tatacgtatc ggtttcgtca gacggtgaaa aattcagatt atcgtaaaat gccgtacgcg
     8341 taatatccgg attgatattc acgactttta cgccttgttt gcggcattca tcaaacaaag
     8401 aagccgaaaa atgccgtaaa cccgctttgg tggcggcata agcggcgccc atacggcttg
     8461 gttccaaggc cgttacggaa gcaatatgaa tgacaaaccc gcgttgttcg cgaatacggc
     8521 gtaaaaaaag atgcgtaaga ataagcggcg ccgtcagatt ggtcgttacc atatcctgca
     8581 gtgtgcgtaa aggaatttcc tcatgcggcg caaaatatcc gaatccggcg ctgtgaatta
     8641 acacatccag cgattcttcc gtcggccatt ttttttcaac ttcttcgagc gcttttatat
     8701 cccgaacgtc acacactatc ggtacgaagt tcgcgtgacg ccagtctgtt ttatcaaaat
     8761 tgcgtgcaag tccataaacc cggtacccca tttcagtaag cttctgcgcc gtggccaatc
     8821 ctatcccgga tgaagcgccg gttatcaatg ccgttttcat aggattatcc tctcctcttg
     8881 gacataagca ctcattgttt tacgaatgta atccagcata ctttcccgat cgagatcagt
     8941 ataactcata gtttgatctt tttcgtcgta cggataaaac aaaatatcgg aatcaacacg
     9001 catagcacga atattattaa gataattttt attcatacga aaaacaccca gacttacatc
     9061 atgcagcgct gcgcccggaa tacgttcgaa tatgtattca atacaacggc tatatatggc
     9121 ttgccaatcc ttgattttca aaatcggatc tatacacaaa cgcactttcc gtccgtcttt
     9181 tacagcttgt gccatcgccg aaagtcttgt atctaatggc ggcgtgccgg attcatatac
     9241 ttttataatc tcatccggtg agcatgtcca tgccaaaatc acgttatctg aaaccggcgt
     9301 atcgcgtatg gcatcataag cgccggattt ggtgcgaatt tcaaccacca gatcccgtcg
     9361 ttgtgcggcg aaatgaatcc agcgatgaca ctgtgggaga aatgactcca tcgccagcaa
     9421 atcggtatcg tacgatacac acaaatacag tggacccttg gccaactcgg ctacgacgct
     9481 atcaaaaaaa gcatccggat tgacaaagaa caaaagatgc gctgacggaa acattccctg
     9541 caaataacaa taatcacaat tgtaaacgca gttgagcacc atcgcattgt aaaaaaatcg
     9601 atcatgcccg aaactcggcg tctgcggtga accggcgtat aaataacctt cgcgtttgac
     9661 ggccagaatg atatccatcg cccgtttttg aagctgaaag tcttgatgct tgcgattgaa
     9721 aacgtcttta taatgtcgta tcggaatgcg tcgggctgtc ggaaaccgcg caagaatagt
     9781 ttgcgcgtcc ggataagctt ctgcgccgtc ctcgatataa atacggctaa aatgtttact
     9841 gaatgagctt ttgtcgaagc cgctccacgc gtgcattgcg ctcctgttta ttttctccag
     9901 cccattgtgt catatccatg agatcaccgg gtaagtaacc attacgaatt ttataactgc
     9961 gtgccgcatc aaaaagcgct atacgctggg catgactggg attgatcatt tcaaaaatat
    10021 gctgcatggt ttcctgttca ttcacactca gtgtttcttt atcaatacgc atcgctgaaa
    10081 gaacatctag acacgaggaa aacttcggca aacattgcat agtaagattt tcgataaaat
    10141 ccgaagtcat acccttgatt tgtaaatgat cggacacgat tttcagaacg tgaatgcgtt
    10201 ctaacggcag aaaacgcgtt gacgcaacaa aaaaaccgct cgcctccata tcatagagcg
    10261 catggtcagc ggaacctaca cactggtttt cttgcggccg atctacggtt cgcaaaacac
    10321 tctcccccat cgtcaaacgg atacttccgt cgggataata atctctgccg gacactacgt
    10381 ccgtgatctt gtgaatgcgc caaaacgagc cgatatcaag tgttgtatcc gatgcgccgc
    10441 agattccgaa attccacaac gtatgaagat ggcctgtcgt gtagaggtac gtagcggccg
    10501 ttgcagattt gatttttccg atcccgctga cgatcagact tatggtttcg ttttcgaaca
    10561 cttcaaacgc cgtatgcgcg atgttttttt tcaatcgcca tgcgcgtatc agcggcgctg
    10621 cttccgggcg taacgcgacg acgataccgt gcttagtgtt ttttttcaca aagctccagt
    10681 aatacgccgc ccgtggactt aggatgcaca aatgctattc tggcgccatg cgcacctgtt
    10741 tttgggactt catcaataag ctgaaatcct ttagccttga ggtcggcgag tttttcctcg
    10801 attcccgtca caccgatcgc gacatgcgca atgccgcctt taccgttatt tttttcaatg
    10861 aatttggtaa tcgggctttc gggatcagta cccgcaagta attcgatcga agattcgccg
    10921 actttaaaaa aagccgtctc gactttttga tccgccacga tttcgcgacc atggtattca
    10981 aatcccatag ccttatataa ttcgatgaat ttttcaagat cgggtactgc aattccgaga
    11041 tgatcgattt tttcgagttt catctgcgtt atccttgttt tgccgcttta agcgtatttt
    11101 tgagtagcat aacgatcgtc atcggaccca caccgccggg aacgggtgtg atggctgacg
    11161 ccacttcttt ggccgaatca aagtgaacat cccctaccaa acgtgatccc gatttggtgg
    11221 acggatcgga tatgcgattg attcccacgt caatcaccac cgcgcccggc ttgagcattt
    11281 cgccgcgtat catttccgca cgccccaccg ccgcaacgac gatatcggct tgcttgataa
    11341 aatacgtcat gtccggtgtt cccgtatggc ataccgtcac ggtagaatta gcgcctttag
    11401 ctttttgcac cagcatattg gccaaaggtt tgcccacgat attgctacgt cctacgatca
    11461 cggtatgttt tccagacgga tcggcttcgg aacgcatgag taattcctgc acgccggccg
    11521 gtgtacatgg caaaaaaatc gcatccccta tcgccaaacg accgacgtta aaaggatgaa
    11581 aacaatctac gtccttggcc ggattgatgg cgttgatgat aagcgtttca ttgatgtgtt
    11641 ttggtaaagg caattgcact aaaatgccgg tgaccgacgg atccttgtta agttgatcaa
    11701 tcagggccag cagatctttt tcaggcatcg tcgcgggttg tttgatcgtt tcatgtgcca
    11761 tgccgacttg ttcacaggcc aaccctttgt tgcgaacata aacttgtgag gccggatctt
    11821 cgccgactaa gacaacgacc aatttgggct gcacattttt tattttaagc gcttcgactt
    11881 cttgcgcaat ttcacgtttg atggattcgg cgatttgttt accatcgatt atttttgcat
    11941 tcacggatgt atcctcgtat atagattcga taatggagtt atttttacaa accgattttt
    12001 tctaatatcc ggccgcatat cccataattc gttgatccgc ttccccgtga taatatccgg
    12061 tccgtggatc aatccagatg gaatgggcat caccgataaa t
//
LOCUS       DAOQNQ010000068        12039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1878_length_12039_cov_5.332360, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000068 DAOQNQ010000000
VERSION     DAOQNQ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12039
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1878_length_12039_cov_5.332360"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="PK754_06190"
     CDS             complement(<1..1305)
                     /locus_tag="PK754_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09126.1"
                     /translation="MSILSSPKMIIPLHAVIAMSYALLHIHCGSSIVPAPTGTPLRLS
                     QLDSLIITSDTIPFLDKQKLCAILGRFPADAWHQKPITIRMYPDFESKWSATKNTIEY
                     SYDHSTRTIHLVDMKLTRVRLNEIIAAYVAELCYGEKYPRLIEGLAILYCGTYYGMPL
                     TWWDKKSALLGLTNKERLITAPENFENPFLYTILAVQFWQKHHDQVHLVFANPDSFIA
                     KFEPALQVAPPAHVFPPDSIPYFKGVCFAHTNGVGSGYMSKQSRTSMRRLKDIGVDAI
                     SLTPFGYAKSTTEPRVHFVLDRHWDETLGGLFKAADDAHDLGIHVMMKPHIWVRGAWC
                     GEIDIRDSTDRIAWENSYAQYATYQGLIAELAGMESMCIGLELPRMTRDTAMWKRIIH
                     HVRIAYSGIITYGGNWFDEYEHIGFWDDLDFISIHAYFPLAHT"
     gene            complement(1400..2989)
                     /gene="serA"
                     /locus_tag="PK754_06195"
     CDS             complement(1400..2989)
                     /gene="serA"
                     /locus_tag="PK754_06195"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNB09127.1"
                     /translation="MLKILISDKLSPAATEIFQRHGITADVRHGLTKEQLIEIIADYD
                     GLAVRSETKVTRDVIERATRLKVIGRAGIGVDNVDVPAATARGIVVMNTPFGNSVTTA
                     EHAVAMMMALARRIPQASTSTHAGKWEKSKFMGVELTGKTLAVIGCGNIGSIVADRAR
                     GLRMRVLAYDPFLTRERAESLGIEKTELDDAIAQADFITLHTPLTDATRHLFDAARLA
                     RCKKGARIINCARGGLIHEPALKSAIDSGHIAGAALDVFEVEPAKENILFGMEHVVLT
                     PHLGASTNEAQENVALQIAEQMSDFLNTGAITNAVNIPSISAAEATALKPYFKLARQI
                     GTLAGQLQQSPIQSITFEYEGHVAALNTKPLSLLLAQGVMAVFDQNVNMVNALYIAKE
                     RGMAVSETKIESEGNYKTLIRLRIVTEKESFSCSGTLFDGDKPRIVEVNGITLEAVLS
                     ASMLFSTNDDKPGFIGNLGKILGDADINIAHFHLGRNQSKTEAMALVTVDSPVRSDVI
                     STIKKLSSVRRVVALDFSGVV"
     gene            complement(3003..4178)
                     /locus_tag="PK754_06200"
     CDS             complement(3003..4178)
                     /locus_tag="PK754_06200"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HNB09128.1"
                     /translation="MSKPSVKPQNTFFSSGPCAKRPGWSADQLPSRTVGRSHRAKVSK
                     AVIEEVIAKSKQILGVPDDYRLGIVPGSDTGAIEMALWNFFGHPSVESRGIDMLAWEN
                     FGYEWVYDVEKQLKIANVRPMVVPYGQIPDLTQVDFHRDVVFTWNGTTSGVCVPDATW
                     IPDDREGLTICDATSAVFAMPIDWKKIDVLTYSWQKSMGGEGAHGVLILSPRAIQRLE
                     SFPPIVAMPKVFTILKKGKLNEEIFTGATINTPSMLCIEDALDSLNWMIREGGLTAMI
                     ARSQQSLSEIKTWIASSPRYAFLAENPKTISNTSVCLKLKSDMYLSLSPDAQVKFAKD
                     VAALLEKEGAAYDIGSYRDAPPGLRIWCGATINPADVKLLLPWLDWAHDEIARTLKF"
     gene            complement(4310..5371)
                     /locus_tag="PK754_06205"
     CDS             complement(4310..5371)
                     /locus_tag="PK754_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNB09129.1"
                     /translation="MSKTTINTDFVQRALKQITENDHGIRHLDKLQEWITQCSDTEAT
                     SINLYRLSESWGVDIRELTALFLWATKFGLFDLHWNIRCPMCNGKTENHDSLGHMHSG
                     SHCVMCKSDFVCELDNTIEVTFSISGNVRPLPENVAEEKCGPADQRLNGFQIINSPIF
                     RKYFSDELLSEQENLSVRHVAIMFTDLKGSTQMYQQLGDARAFKIVRQHFDLLEQAIH
                     AHGGVIVKTIGDAVMASFAKTSEAVGAAIDAQKQFRTFNESMQLQDGVTIRIGIHVGN
                     SLMVTLNNRLDYFGTMVNIAARVEALGDGNEILITRNVFDDLGVKNELLSRAQSIQPF
                     TARLKGIRDEQKVMKVVYN"
     gene            5524..6207
                     /locus_tag="PK754_06210"
     CDS             5524..6207
                     /locus_tag="PK754_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09130.1"
                     /translation="MLKNIILSAFVLSLISCGKPSNIEEFQRLVQKVSKKNEEIGSIN
                     MEIAEAVRKYNESRKADEQPIVLPDSMMGLNKEQLKLIQDMISKEQDISTKGMLTQII
                     DKNKTIEKLNADLEDMKAKLPRPVVVKAGDTHHKIGYDFLTKEKGVDPKRANELLEQS
                     FLADDLLPGFNVWVYYNDDVFGTFVNQGNVRISPNQFSRIIRKKQMDDAREAGRQDAT
                     EKPAEPAAK"
     gene            complement(6299..7804)
                     /gene="accC"
                     /locus_tag="PK754_06215"
     CDS             complement(6299..7804)
                     /gene="accC"
                     /locus_tag="PK754_06215"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNB09131.1"
                     /translation="MSITHRTIKKILIANRGEIAIRVIRACREMNIKTVAVFSEADRT
                     AAHVRLADEAYCIGEPPSNKSYLRMDKIIEVAKKSGADAIHPGYGFLSENEDFADLVI
                     QNGIIFIGPSSEAMAQMGSKTAARTLAKKMNVPTVPGTESGIKDKNEAITIAEKIGFP
                     VLIKAAAGGGGKGMRVVQKIEELSEAIDRARGEALNAFGDDTVFIEKYVTKPRHIEIQ
                     IIGDQHGNMVYLGERECSIQRRHQKVIEEAPSAIVTPEMRKRMGESAVRLAQAVNYYN
                     AGTMEFLVDADMNFYFLEMNTRLQVEHPVTEMVTGIDIVKEQIRIASGEPLSFRQEDI
                     RIHGHAIESRIYAEDCENNFAPSIGRIEHLEPSYGPGIREDSGVFEGDTIQIYYDPMI
                     SKLAAWGPTREEAINRMRRSLREYAIVGVETTIPFCLFVMENEKFRSGDFDTSFVGKE
                     FTPEKLQHKEEKMAAIAAAVHDFAKKRDMKISTDALTASSPKSSRWKRNRN"
     gene            7922..8683
                     /locus_tag="PK754_06220"
     CDS             7922..8683
                     /locus_tag="PK754_06220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB09132.1"
                     /translation="MSASTAYPEFVARFYDTVYAKIRTSIDHDFYVEETTRAQGSVLE
                     VGVGTGRLFMEALRAGADIYGLDASATMIRTLQAKLDAKEKQRVSVADVRDFTMDRRF
                     ALIVAPFRVFTHLRTVDEQLAALACIKDHLEPGGTLILDVFNPDPVLCSQGRESTLDF
                     DGEWAPGQRLKRFTTVVPDFMHQINHVTMRYVWDENGTTHDESWSFRVRYYFRFELEH
                     LIYRSGLALETMYGGFDRTAFDENSKELIAVCKSN"
     gene            complement(8690..9574)
                     /locus_tag="PK754_06225"
     CDS             complement(8690..9574)
                     /locus_tag="PK754_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNB09133.1"
                     /translation="MTWKKVLLYFFIVLFSAATGGGIVGYYAYEKYYRPMDLDLWKTR
                     NALAESHAQLQAIYPEYEHAKQFADSLQTFIPLQSGLNYPMESERQYYYHDKMFNIIY
                     DTKRYLEQKRRKQESHFLELVDHVMPHEKHLRMILDSIGVKTNDTLHLEKNAQAIMDF
                     VQCIPYQSENRAYTKMPLETLYEGGGDCEDLAVLAYSMLTTAGIEAVLVVPTRTIASI
                     DHATVAIHGNFTGQYIEHNQKRFFIAESAGTTDARKPKRFKIGRAEKYNISLVLGDSL
                     EVAKHEVDWIIKEKLRTR"
     gene            complement(9576..11651)
                     /locus_tag="PK754_06230"
     CDS             complement(9576..11651)
                     /locus_tag="PK754_06230"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNB09134.1"
                     /translation="MSNFFRSIFSSKPKPPVAAIKPHHVTHIGKSLTDDYYWLRDKEN
                     PEVIAYLEAENTYTDALMKPTEALQDTLYREMLSRIREDDRSVPEQIGEFLYYYRTEK
                     DKQYKIYCRKKNGEENEAVLLDLNTLARHTPYLALGAYRVSPDHHWLAFTIDHDGSEQ
                     FTLYIKNLETGELSPEHIPNTYYGLEWANNSATIFYTTLDPSLRPHRVYRHSIGAELT
                     SDQLVYEESDERYYLDLTKSRSGRFLLITLASKTTTEVHFLDADHPDKPFQVFQERVQ
                     GHEYNVEHWEDRFFITTNDQAVNFRLMETPADRTNKQNWREVIAHRDDALLLGVDAFR
                     DFIVIHTRTNGLKSITIRTQPDRAEHTVLFPEPVYSYTPLRNPDYNASFFRLNYTSLV
                     TPSTVMDYDIKKRELTIRKQEEVAGYDANDYSSERILVQATDGTLIPMSIVYKKDRLP
                     NKPTPALLYGYGSYGICIDPQFASHRISLLDRGFVFAIAHIRGGDDLGRKWYEDGKFL
                     HKKNTFTDFIACARHLISQGYTTPAQLAIMGGSAGGLLMGAVINMQPDLFGAVVAHVP
                     FVDVVNTMLDPNLPLTITEYEEWGNPNDPAYFDYMMSYAPYENVKRQAYPHILVTAGL
                     NDPRVSYWEPAKWVAKLRRMKTNRNDLLMRTHMDFGHSGASGRYDYLLEIAFDYAFII
                     EKTKPSGRY"
BASE COUNT         3123 a         2704 c         2897 g         3315 t
ORIGIN      
        1 ggtgtgtgcg agcgggaagt aggcgtgaat gcttataaaa tcgagatcat cccaaaagcc
       61 gatgtgttcg tattcatcaa accaattccc gccgtacgtg atgataccgc tgtatgcaat
      121 gcgtacgtga tggatgatac gtttccacat ggcggtatcg cgtgtcatcc ggggcagctc
      181 aagacctata cacatggact ccataccggc aagctccgcg atgaggcctt gatacgtcgc
      241 atattgagca taggaatttt cccatgcgat ccggtcggtt gagtcacgaa tatcaatttc
      301 gccgcaccag gcgccgcgta cccagatatg aggtttcatc atgacatgga tgccaaggtc
      361 atgcgcatca tcggcggctt tgaataaacc tccaagcgtc tcatcccaat gacgatctaa
      421 aacaaaatgt acgcgaggtt cggtcgtgct tttcgcataa ccaaatggtg tgagtgaaat
      481 ggcgtcaaca cctatatcct tcagtcgccg catcgaagtc cgggattgtt tggacatata
      541 gccgctgccg acaccgttag tatgtgcgaa acaaacacct ttgaaatacg gaatgctgtc
      601 gggcggaaac acgtgtgccg gtggcgccac ttgcaaagcc ggttcaaatt ttgcaataaa
      661 ggagtcgggg tttgcaaaga ccaaatgaac ttgatcgtgg tgtttttgcc aaaactgcac
      721 agctaagatc gtgtacaaaa aaggattttc aaaattttca ggcgcagtta ttaatcgctc
      781 tttgtttgtc aggccgagca atgctgattt tttatcccac caagttaacg gcattccgta
      841 atatgtcccg caatacagta tggcgagccc ctcgatgagg cgcggatatt tttctccgta
      901 acacaattcg gcaacgtatg ccgcaataat ttcattcaat cgcacgcggg tcagtttcat
      961 atccacaagg tggatagtac gtgtactgtg atcgtatgaa tattcgatcg tatttttagt
     1021 ggcgctccat ttggattcaa aatcgggata catccgaatc gtgatcggtt tttgatgcca
     1081 cgcatctgca ggaaaacggc ctaatatcgc acagagtttt tgtttgtcta aaaaaggaat
     1141 cgtatcggat gttatgatga ggctgtccag ctgtgacaaa cgaagcggag ttccagttgg
     1201 tgcaggcacg atagaagagc cgcagtgaat atggagcaac gcgtatgaca tggcgattac
     1261 tgcatgtaat ggaataatca tctttggaga ggacaaaata ctcatgaggg aactttctgt
     1321 aaaagttctt tgcattgata acacacaaag ctaaccgcga ggggtttgat aaatgatgta
     1381 agccgaattc aattaaaatt taaacgacgc ccgaaaaatc taatgcaacg acacgtcgta
     1441 cactggacaa tttttttatc gtactgatga catcagaacg aacgggcgaa tcgacggtta
     1501 cgagcgccat agcttcggtc ttgctttgat tacgaccgag atgaaagtgg gctatattga
     1561 tatctgcgtc gcctaaaatt tttccaagat tgccgataaa acccggtttg tcatcattgg
     1621 tgctgaaaag catggatgcg cttagaacgg cttccagtgt gatgccgttg acctctacga
     1681 tacgcggttt gtcgccgtca aagagtgtac ctgaacaact gaatgattct ttttcggtaa
     1741 cgattcgcaa gcgaattagg gttttgtaat ttccttcgga ctcgatttta gtttcactta
     1801 cggccatgcc gcgttcttta gcaatatata acgcgttgac catgttgacg ttttgatcaa
     1861 atacggccat gacaccttgc gctaaaagta aactgagcgg tttagtattg agcgccgcaa
     1921 cgtgtccttc atattcaaag gtgattgatt gaataggcga ttgctggagt tggcctgcta
     1981 atgttccgat ctggcgcgct aatttgaaat agggtttgag cgccgtggct tcggcggcgg
     2041 atatggaggg aatattcacc gcattggtga tcgcacccgt gttgagaaaa tcggacattt
     2101 gttccgcgat ttgcaatgcg acgttttcct gagcctcgtt ggttgaagcg cccaaatggg
     2161 gcgtcagcac gacgtgctcc atgccgaata aaatattttc tttggcgggc tcgacttcaa
     2221 acacgtccag tgcggctccg gcgatatgtc ccgaatcgat ggccgatttc aatgccggtt
     2281 cgtgaataag tccgccgcgt gcgcagttga taattcgtgc gccttttttg caacgtgcta
     2341 atcgtgccgc atcgaaaaga tggcgtgtcg cgtcggtgag cggtgtatgc agtgtgataa
     2401 aatcagcttg cgctatcgcg tcgtccaatt cggttttttc aatgccgagc gactccgcac
     2461 gttcccgtgt taaaaacgga tcatacgcta aaacacgcat tcgcaatccg cgtgcgcggt
     2521 cagcaacaat gctgccgata ttaccgcaac cgattacggc aagtgttttt ccggtcaatt
     2581 ccacacccat aaatttactt ttttcccact tgccggcatg ggtggaagtg ctggcttgcg
     2641 gaatgcgccg ggcaagcgcc atcatcatag ccacggcgtg ttcggccgta gtgacagaat
     2701 tgccaaacgg cgtattcatc acgacgatac cgcgtgccgt ggccgccggc acatccacat
     2761 tgtccacgcc gataccggcc cgtccgatga ctttgagccg tgtggcgcgt tcgatcacgt
     2821 cgcgtgtgac tttggtttca gagcgcacgg caagtccgtc gtaatccgcg attatttcga
     2881 tcagttgctc tttggtcaat ccgtggcgaa catccgccgt aatgccgtgc cgttggaaaa
     2941 tttccgtagc ggcagggctg agtttgtcgg agataagaat tttaagcata atatttttcg
     3001 gtttagaatt tcaaagtacg tgcaatttcg tcatgagccc aatcaagcca gggaagtaat
     3061 aattttacat ccgccggatt gatcgtcgca ccgcaccaga tgcgcagacc gggaggtgca
     3121 tcacgatagg aaccgatgtc gtaagccgca ccttcttttt cgagcaaagc ggcaacatct
     3181 ttggcaaatt tgacttgtgc atccggagac agcgacagat acatatcgga cttcaatttt
     3241 agacagacgg atgtattgga aatcgttttc ggattttctg caagaaaagc ataccgcggc
     3301 gatgaggcaa tccatgtttt tatttccgat aagctttgct gcgaacgcgc gatcatagct
     3361 gttaatccgc cttcacggat catccaattt aatgagtcca aggcatcttc gatgcacaac
     3421 atggatgggg tattgatcgt ggcaccggtg aagatctctt cgttgagttt tccttttttt
     3481 aaaatcgtaa aaactttggg catggcgacg atcggaggaa aagactccag ccgctgaatg
     3541 gccctcggcg ataggatcaa aacaccatgg gcaccttcac cgcccatgga tttttgccac
     3601 gaatacgtga gcacatctat ttttttccag tctataggca tggcaaatac ggccgaagtc
     3661 gcatcgcata tcgtcagacc ctcacgatcg tcaggtatcc acgttgcatc aggaacacaa
     3721 acaccggagg ttgtgccgtt ccacgtaaaa accacatcgc ggtgaaagtc aacctgcgtg
     3781 agatccggga tttggccgta tggaactacc atcggacgta cgttggctat tttcaattgt
     3841 ttttcgacat cataaaccca ttcataccca aaattttccc aagccagcat atcaatgccc
     3901 cgcgattcta cggaagggtg gccgaaaaaa ttccataacg ccatttcgat ggcaccggta
     3961 tccgagccgg gtacgatccc gagccggtaa tcatcgggaa caccgagaat ttgtttggat
     4021 tttgcaatga cttcttcgat tacggccttc gaaactttag cgcgatgaga acgtcctacg
     4081 gtacgtgacg ggagttgatc ggcgctccaa cccggacgtt tggcgcaagg accggatgaa
     4141 aaaaatgtgt tttgaggttt gacggatggt ttgctcataa aggatgtact tttattgtaa
     4201 tgggtatata gagaacacaa aaagcgtgat cgcatgcatc acgctttaat ataacattct
     4261 ctcaataaaa atatgcaaat tattatgagc gcataacgaa cggtaaaaat tagttataca
     4321 ctactttcat gactttttgt tcgtcgcgaa ttcctttgag ccgcgcagta aacggctgaa
     4381 tactctgcgc gcgtgacagc aattcgtttt ttacgccgag atcatcaaat acattgcgcg
     4441 tgatcagaat ttcattgcca tccccaagcg cttcgacacg ggcagcgata ttgaccatcg
     4501 taccgaaata atcaagccgg ttattgagcg tcaccatcaa agaattaccc acgtgaatac
     4561 cgatgcgtat cgtcacgccg tcttgtaact gcatgctttc gttaaacgtg cggaattgtt
     4621 tttgggcatc gatcgcggcg ccgacggcct cggatgtttt agcaaaactg gccatgacgg
     4681 cgtcgccgat agttttgacg atcacaccgc cgtgagcatg gatggcttgt tcgagtaggt
     4741 caaaatgttg ccgaacgatt ttaaaagccc gcgcatcgcc gagttgctga tacatttgcg
     4801 tcgaaccttt gagatcggta aacatgattg caacatggcg aacggacaaa ttttcctgct
     4861 cggaaagaag ttcatcggaa aagtacttac gaaaaatcgg tgaattgatg atctggaatc
     4921 cgttcaaccg ttgatcagcg ggtccgcatt tttcttcagc cacattttca ggcagcggac
     4981 gtacgttacc actgatgcta aatgtcactt cgatggtgtt atcaagctca catacaaaat
     5041 cgcttttgca catcacacaa tggctgcccg aatgcatatg cccgagcgaa tcatggtttt
     5101 ccgttttgcc gttgcacatc gggcagcgaa tattccagtg cagatcaaat aacccgaact
     5161 tggtggccca taaaaaaaga gcggtcagtt cgcgtatatc cacgccccac gattcggata
     5221 atcggtataa attgatcgaa gtcgcctcgg tatcgctgca ttgagtgatc cattcttgta
     5281 gtttatctaa atggcgtatt ccatggtcat tttccgtgat ttgtttcagc gctcgctgta
     5341 caaaatcggt attgatagtt gttttagaca tgatcctctc cttataaagt acacgcttac
     5401 taacgtacag tcaggtgatt tattccgtga ttaaatttcc ttgactttgg ctgtgctaaa
     5461 acctacaatc gcgcgttagt gaaccctaaa cccaaataac tcatgtattg aggagaaaaa
     5521 accatgttaa agaatatcat cctgagcgcg tttgtgctta gtctgatcag ttgcggcaag
     5581 cccagcaaca tcgaagaatt tcagcgttta gtacaaaaag tcagcaagaa aaacgaagag
     5641 atcggctcca tcaatatgga aattgcggaa gccgttcgta aatacaacga gtcccgcaaa
     5701 gccgacgaac agcctatcgt tttgccggat tctatgatgg gattgaataa agagcagctc
     5761 aagctgattc aggacatgat ttccaaagag caggatattt cgaccaaagg catgctcacc
     5821 cagattattg ataaaaataa aaccatcgaa aaactcaacg cggatttgga agacatgaaa
     5881 gccaaactgc cgcgtcccgt cgtcgtcaaa gccggtgata cgcatcacaa aatcgggtat
     5941 gattttttga cgaaagaaaa aggtgtagat cctaagcgtg cgaatgaatt gctggaacaa
     6001 tcctttttag ctgatgatct tttgccgggc tttaatgtat gggtgtatta taatgatgat
     6061 gtgttcggta cgtttgtcaa tcaaggcaac gtgcgtattt cgcccaatca gttcagccgc
     6121 attatccgta aaaaacaaat ggatgatgcc cgtgaagccg gtcgtcagga cgctaccgaa
     6181 aaacccgcag agcctgcagc caagtaatat ttgaaaatga agtaaaaaaa ggcggccgaa
     6241 gagccgcctt ttttgttatg ctaaatacat tctgttatgc ataccgcaaa aacccatgtt
     6301 agttgcggtt tcgcttccat cggctggatt tgggagagga agccgtcaat gcgtccgtgc
     6361 tgattttcat atcacgtttt tttgcaaagt catgaacagc agcggcaatg gcggccattt
     6421 tttcttcttt atgctgaagc ttttccggag tgaattcctt tcccacgaaa ctggtatcaa
     6481 aatcgccgga gcgaaatttt tcgttttcca ttacaaaaag acaaaacgga atcgtggttt
     6541 cgacgccgac aatcgcatat tcacgcaagg aacgtcgcat gcggttaata gcttcttcac
     6601 gtgtgggtcc ccaggccgct aatttagata tcatcggatc ataatagatc tggatggtat
     6661 cgccttcgaa aacgccgctg tcttcgcgaa tgcccgggcc gtacgacggt tccaaatgtt
     6721 cgatgcggcc gatggacggt gcaaaattat tttcgcaatc ttcggcgtaa atgcggcttt
     6781 cgatagcatg gccgtgtata cggatgtcct cctgtcggaa ggaaagcggt tcgccggatg
     6841 caatgcgtat ctgctctttg acgatatcaa tgccggtgac catttccgtg acgggatgct
     6901 cgacttgcaa acgtgtattc atttcgagaa aataaaaatt catatccgca tccacgagaa
     6961 actccatcgt accggcattg tagtaattga cggcttgcgc caaacgaacg gcggattcgc
     7021 ccatacgttt acgcatttcc ggtgttacga tggccgaggg cgcttcttcg atcactttct
     7081 ggtgacggcg ctgtatggag cattcgcgtt cgccgagata caccatgttg ccgtgttgat
     7141 cgccgatgat ttggatttcg atgtgccgcg gtttagtgac atatttttcg atgaaaacgg
     7201 tatcgtcacc gaaggcattg agcgcttcgc cgcgtgcgcg atcgatcgct tcgctgagtt
     7261 cttcgatttt ttgaaccacg cgcatgcctt taccgccgcc gccggccgcc gccttgatca
     7321 aaacgggaaa gccgattttt tcggcgatcg tgatggcttc gtttttatct ttgataccgg
     7381 attcggtacc gggcacggtc ggaacattca tttttttggc cagcgttctg gccgcggttt
     7441 tactgcccat ctgcgccatc gcttccgacg acgggccgat aaaaataata ccgttttgaa
     7501 tgaccagatc ggcaaaatct tcattttcac tgaggaaacc gtatccggga tgaatcgcat
     7561 ccgcgccgga ttttttggcg acttcgatga ttttgtccat gcgcagatag cttttattgg
     7621 acggcggttc gccgatacaa taggcttcat ccgctaaacg aacgtgtgcc gccgtacgat
     7681 ccgcttcgga aaaaacggcg acagttttta tattcatttc ccggcatgcc cggatgacgc
     7741 gtatggctat ctcaccgcga ttggcgatga gaattttttt gatagtgcga tgggttatgc
     7801 tcataagtat tatagttctt tcagaaaagc atttttgata aattgataaa attatccttg
     7861 cacccgtaaa tttaacccaa tagttttcat tattcaatag ccaactccaa aggagatacc
     7921 tatgtcggct tctacggctt atcctgaatt tgtagcacga ttttatgata cggtgtatgc
     7981 caaaatacgt acatccatag atcatgattt ttacgtcgaa gaaacaaccc gcgcgcaagg
     8041 ttctgttttg gaagtcggtg tgggaacggg acgacttttt atggaagcgt tgcgtgccgg
     8101 tgcggacatc tacggactgg atgccagcgc gacgatgatt cgcaccttac aagccaaact
     8161 tgatgccaaa gaaaagcaac gcgtgagtgt cgcggatgtg cgcgatttta cgatggatcg
     8221 ccgttttgcg ttgattgtcg cgccgtttcg cgttttcacg catcttcgta cggtggatga
     8281 gcaacttgcg gcgttggcct gcatcaaaga tcatttggaa cccggcggta cattgatttt
     8341 ggatgttttt aatccggatc cggtattgtg ctcacaagga cgtgaatcca cgttggattt
     8401 tgacggggaa tgggcgcccg gccaacgtct caaacgattt accaccgtcg tcccggattt
     8461 catgcaccaa atcaatcatg tgacgatgcg ttatgtctgg gatgaaaacg gtacgacgca
     8521 cgatgaatcg tggtcattcc gggtacgtta ttatttccga tttgaactgg agcatttgat
     8581 ttaccggagc gggttggcgc tggagacgat gtacggcgga tttgatcgca cagccttcga
     8641 tgaaaactca aaagaactga ttgccgtttg taaaagcaat tgaggttgtt taccgggttc
     8701 gcagtttttc tttgatgatc cagtccacct cgtgtttggc aacttcgagg ctgtcaccga
     8761 gtacgagcga aatattgtat ttttccgcgc gaccgatttt gaaacgtttg ggtttgcgtg
     8821 cgtcggtcgt tccggcggat tcggcgataa aaaaacgctt ctgattatgt tcaatatatt
     8881 ggcctgtaaa atttccatga atagcgaccg ttgcatgatc tatggaggca atcgtacgtg
     8941 tgggtacgac cagtaccgct tcaatgccgg ccgtcgtaag catcgaatac gctagaacag
     9001 ctaaatcttc acaatctcct ccgccttcgt ataacgtctc cagcggcatt ttagtataag
     9061 cacggttttc ggattgatac ggtatgcact gaacgaaatc catgatggcc tgtgcatttt
     9121 tttccagatg aagcgtatcg tttgttttta cgccgatact gtcgaggatc atgcgcagat
     9181 gtttttcatg cggcatcaca tgatctacta attccagaaa atgcgattcc tgtttgcggc
     9241 gtttctgctc cagataacgt ttggtatcgt agatgatatt aaacattttg tcgtggtaat
     9301 aatactgacg ttcgctttcc atcggataat taagtccgct ctgcagtgga atgaatgttt
     9361 gcagcgagtc ggcaaattgt ttcgcgtgtt cgtattcggg ataaatagcc tggagttgcg
     9421 catgggattc ggctaatgcg ttgcgtgttt tccataaatc aagatccata ggacggtaat
     9481 atttttcata cgcataataa ccgacgatcc cgccgccggt ggctgccgaa aacaggacaa
     9541 taaaaaaata aagtaaaact tttttccacg tcatatcaat agcgtccgga cggtttggtt
     9601 ttttcaataa taaaagcata atcgaaagcg atctctaaca aataatcata acgacccgaa
     9661 gcgccgctgt ggccaaaatc catgtgggtg cgcattagaa ggtcgttgcg attggttttc
     9721 atacgccgca gtttggcaac ccacttggcc ggctcccaat aacttacgcg tggatcattg
     9781 agcccggccg ttacgaggat atgagggtac gcttgacgtt tgacattttc gtaaggcgcg
     9841 taagacatca tatagtcaaa ataagcggga tcattggggt tgccccattc ttcgtattcg
     9901 gtgatggtca gaggcaagtt gggatcaagc atggtattga ccacatccac gaaaggaaca
     9961 tgggctacga ccgctccgaa gagatcgggt tgcatattga tcacggcgcc catcagaaga
    10021 ccgccggcgc ttccgcccat aatggctaat tgagcgggtg tcgtatagcc ttgcgaaatc
    10081 agatgccttg cgcaggcgat aaaatcggtg aacgtatttt ttttgtgaag aaattttccg
    10141 tcttcgtacc atttgcgtcc gagatcatcg ccgccgcgaa tatgagctat ggcaaaaaca
    10201 aaacctcggt ccagtaaact gatgcgatgt gaagcaaatt gcggatcaat acaaatgccg
    10261 tatgaaccgt aaccatataa tagtgcaggt gtcggtttat tcggtaagcg atcttttttg
    10321 taaacgatgg acatgggaat taacgtgcca tccgtcgctt gtaccaaaat gcgttcgctg
    10381 gaatagtcgt tggcatcata acctgccacc tcttcctgtt tacgaatcgt tagttctctt
    10441 ttcttaatgt cgtaatccat caccgtggac ggcgttacaa gcgaagtata gttcaaacga
    10501 aaaaacgatg cgttgtaatc cggattgcgc aaaggcgtat aactatacac gggctccgga
    10561 aaaagtacgg tatgttcagc tcgatcgggt tgcgtgcgaa tggttatgct tttgaggccg
    10621 ttggtacgtg tatgaataac tataaaatcc cgaaaagcat cgacacccaa taaaagtgca
    10681 tcatcccggt gagcaatgac ttcgcgccaa ttttgtttat ttgtccgatc tgcaggtgtt
    10741 tccatcaggc gaaaattaac agcctgatca ttggtcgtga taaaaaagcg atcctcccaa
    10801 tgttcgacgt tatactcatg accttgcaca cgttcctgaa atacttgaaa cggtttgtcg
    10861 ggatgatccg catccaaaaa atgcacttcg gttgttgttt tactggccaa tgttatcaaa
    10921 agaaaacgcc cgctgcgcga tttggtaagg tcaagataat accgttcatc ggattcctcg
    10981 tacacaagtt gatcggatgt taattctgcg ccgatactgt gccgatacac ccgatgcggg
    11041 cgaaggcttg gatcaagcgt cgtataaaaa atcgttgcac tgttgttggc ccactccaaa
    11101 ccgtaatacg tattcggaat gtgttcgggg gataattctc cggtttctag atttttgatg
    11161 taaagtgtga attgttctga tccgtcatga tctatcgtaa atgcgagcca atggtgatcc
    11221 ggactgacac ggtaggcgcc gagtgcaaga tagggtgtat gccgggccag agtgttgagg
    11281 tcaagtagta cggcttcgtt ttcctcgccg ttttttttac ggcaatagat tttatactgc
    11341 ttgtccttct ccgtccggta ataatataga aactcaccga tttgttcagg gacggatcgg
    11401 tcgtcctccc gaatgcggct gagcatttcg cgatacagcg tatcttgcaa agcctctgta
    11461 ggcttcatca aggcgtctgt atatgtattt tcagcttcca ggtaagcaat aacttccggg
    11521 ttttctttgt cacgtagcca ataataatcg tctgtaagtg atttaccgat atgcgttaca
    11581 tgatgcggct ttatggctgc taccggaggt ttgggttttg aggagaagat agaacgaaaa
    11641 aaattactca tggtttgtgc aattaaaata tgaccgtatg aaaaaaatgg aactcaaagt
    11701 aaaaaatgat cggtagtttt gacttctcta ttaccttttt taatcaaagc ggctcaatat
    11761 acattcataa caaattatat catatagctt tttaagtcat ggaatcttga attaatagta
    11821 ggtacgaatc tttaaaatta gttgaaatta tgcaaacgga gtaatggctt attgatcacc
    11881 gccgattgtc tctacactaa gaaaataaag catggctact gcggtattca tacatgcatg
    11941 ccattttttt atggcagtgt gaaagttttc aatcaatttt aaaacaatga taactttatt
    12001 ataagagata attcattgaa aattttaggg acagttata
//
LOCUS       DAOQNQ010000069        12038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1879_length_12038_cov_5.255362, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000069 DAOQNQ010000000
VERSION     DAOQNQ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12038
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1879_length_12038_cov_5.255362"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="PK754_06235"
     CDS             <1..938
                     /locus_tag="PK754_06235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB09135.1"
                     /translation="CRSTAVLAFLPGRWIGTIHGGGILRSDEPRNFFVKLSSWLGGLF
                     IRRADAIVTISNEAEKLIRNSVSDKILQSKLKVIYNGIEFNPAVVKTKEDALTFCPQW
                     RNRKIVLTVGRLVEAKGHDLVIQSIHRLRSEIPEILYIIVGEGNTRKNLEMLVKKLGL
                     ENYVFFAGYVESSLLENYYAVADIFTMPGRVTREFVEGFGLVFVEAGMRGKAVIGTRV
                     GGITEAIRDNYSGLLIAPDDVENLSEAMKKLLICPATAAVLGRQGREWTINNFSLDVM
                     ARHNDRLLLEIYWRKKGRNSLPTNLISTNAMEFSK"
     gene            1554..2384
                     /locus_tag="PK754_06240"
     CDS             1554..2384
                     /locus_tag="PK754_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HNB09136.1"
                     /translation="MKAFILAAGQGKRLLPLTAETPKTLVDVKGTTILEYQLVTLASC
                     GVKDVVIIGGFEIEKLKNYAERYIAIHGLDIRLKMVNNKDYATTNNLFSLWLAKDEMT
                     TEFVVINGDNVFERKALAKVIAQKDMNVSIAIHKNPAYDNEDMKVRINGDRVIEISKE
                     IDNYAANGESIGLRAFRNSGIEAFKQALDSAMIKDVTRNAFFVRAIQHMIDMGENVGW
                     VDVTDFKYGELDFFEDLHHLEIEMSGLMKQTIMLHTNPAGLYARTNGLYQPLMMKNVG
                     "
     gene            2509..3696
                     /locus_tag="PK754_06245"
     CDS             2509..3696
                     /locus_tag="PK754_06245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB09137.1"
                     /translation="MSFQNDDHTLFSIAFIAGVPIPSNDVVSQQFINNVAAIMSVNPS
                     WRVKVFAEKPTVGFENVNVQEHYGIAQDIPIEYITVPTLPKKWRKHYSACKAILAAGR
                     EKFDFYFTRHPETLLWSLLAGHKTFFDTYRINLNQRAKYLLWRKYCYTRPHFCGVIAH
                     SKLCAESFLNVCLASSKITIAYNGSHPGRQMDGNETMVLRRQLNIDDGKLVICYAGRI
                     SDSSKGSGMLLDLAQQLPNIIFLFVGGGKKERDESDFDNKIRTLRLSNVRRTGWVEPS
                     AVSRYLQLADILLIPPTAAGLEKKGNTVLPIKTFQYLQAGRAIVAPDLPDCREILQNN
                     QNAILVRPDDLDAARKAVQVLSENSDLRISLSRNAYRDGLKYTWTERAKFISAFIRTR
                     SAS"
     gene            4004..5161
                     /locus_tag="PK754_06250"
     CDS             4004..5161
                     /locus_tag="PK754_06250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB09138.1"
                     /translation="MNNPPRIAVFKTKFLPYSETFIHDELHAHQRYKPFVLAKKHLNA
                     AQFTDHEVINFLPSDNLLNRIEHYLFNRWRWSWTFKQVLIRNRVCLIHAHFAYSGIFM
                     VPLAKKLNIPLIVTLHGNDVAILLGDQLHQKKWALYKKKYKELLHQASLFLADSQELK
                     DRIVEIGCPAEKVIVHTLGIDLKKFPYQAQKPSLPPRILMVGRFVEKKGFIYGIRAFA
                     AARKDIKNAELHIIGDGELRTEYEAEARSLGVASTVFFHGVRPHEEIVQHMHQAALFL
                     APSVIAANKDRDSGLIVAKEAAACGLPVIGSIHGGIPDIIADGETGFLIPERDVIKMS
                     QCIVEILNNPELWSRMSIASRKKIEEEYNIEKCNERSSSIYDELLKSMQAM"
     gene            5321..5905
                     /locus_tag="PK754_06255"
     CDS             5321..5905
                     /locus_tag="PK754_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HNB09139.1"
                     /translation="MRQDISSRIKESITTKEKILSDEVLIANIEKVTDIIISAFRSGH
                     RLYLCGNGGSAADAQHIAAELSGRFYYDRKPLYAEALHVNSSYVTAVANDYGYDVVYE
                     RMVDAAGQAGDVLIGISTSGNSPNVVKALQKAKVLNMHTVGMTGANGGVMKGICDILI
                     NIPSKDTPRIQECHILVGHILCELIEQTLFPKPK"
     gene            5962..7164
                     /locus_tag="PK754_06260"
     CDS             5962..7164
                     /locus_tag="PK754_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNB09140.1"
                     /translation="MLERLLRQLHENNVTEARILISEDQSHHLSFRREFSSRYVMHLT
                     YIPTRETITQYLSKAKISSDVLLLNAHVLHDLRIIKKLVDSTNSLIIVDENHSGPCAA
                     KFIHDISIVYDNSGQDIELRKTLLILAQQKSIEVLRVSEMQSYVRFLRRHATPLFQSI
                     TPSTDMNALEHIMYEDTFKGGMEWIAVYGYKIPVRELTKWCARTSWITPNVITALATI
                     GKLVAIPFFFIGWIGWALLSFWIFTIGDSLDGKLARMTVRFSKTADRVDHLTSAPARM
                     LWHFGLTWYMCGGNLTSPIGMAGIGFSLLPQIDKFGLMYFNWKFGRSPIDFTSFDARV
                     HLYTARRNDMVLLTIGYLAGFLLPAFYGMMAWMYLTWLWHVVRISWFSFINPVTRGSV
                     FKKHTTHL"
     gene            7161..8321
                     /locus_tag="PK754_06265"
     CDS             7161..8321
                     /locus_tag="PK754_06265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB09141.1"
                     /translation="MRVFILAMKYLPASGGTATYAHNLAQGLARMGHDVLLVSPDYAR
                     PQSDDGLPYRVIRLSQARLGLGQFRIFLTAYHTKKILNNFNPDVIWASTYAGCRVLGL
                     LRLRIPTVGTIHGGGIQRAFSSLNPIKRCTDHAGWRFMRRASAMITVSEEAKKLVCSA
                     IQDTLIQSKFRVIYNGIDFDPSSVVSKKEALSRWPQFEGRIILLTVGRLVKAKGHDVV
                     IRALPEIQKRVPNILYVIAGEGVEKEQLINLVESLSLKNSVHFTGYITPTGLEELYAI
                     SDLFVLAGRWTPTFVEGFGLVLVEAAIRGKPVIGARIGGIPEAIEDGVSGIVIEPEDP
                     SQVAKAVMDILSDQNLYDRMAKAGPVWVNLHFTQAIMARNIDQLLCYITGKS"
     gene            8321..9616
                     /locus_tag="PK754_06270"
     CDS             8321..9616
                     /locus_tag="PK754_06270"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HNB09142.1"
                     /translation="MASLLVQSGRILLGRASISVISIFFTIYYARTLSRTQFAFIVLY
                     ETAAALFMSLTELGMYSKVVREAPPLLRDHKESFVIDHIIMPATVTRAMFGFFLSLLY
                     GMTAWIFEAELPAELRALNMAWVIVFACIGIFFEGLGTITEAIFAVKQRFGMNSFLGS
                     AQTLVEQIFALLFFVQFGLTYYFVGVSIAQAVMFLLRIWYIRDVVFQFRPNFYRWNSA
                     KKMLKEYFPFYVRRFFRLGFLQGEQIAVAFLLPLEQLANFNLAKRTAKFMKPYIEAFL
                     NPLIIKLSKARDITLMKEYRKTFLWFTMPVPVILALASPWIIQIYGEKYAASWPIMAI
                     FSLSWIFYALSTLQLGVVGIFGEPKEALMADAFAGIFGLSTTILLILLFKEYGLAWGQ
                     VISFATLYISGLYIARKYYRNPQLIKPVTVQPITQENPS"
     gene            9613..10263
                     /locus_tag="PK754_06275"
     CDS             9613..10263
                     /locus_tag="PK754_06275"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNB09143.1"
                     /translation="MIRLLNLANSITIARIGVLYITVVLIYTQRPMWLIIAVLLAILI
                     IVMDALDGYVARKRNEVTQFGGVLDITGDRIVENVFWIVFADLDIIPMWIPIIVMSRG
                     FMTDAIRSQALADGKTAFGENTMMQSEIGKFLVSGRFMRAFYGVIKAITFPYLILVLL
                     AKEQHLRDANLQDYLWLSSLTYNGGLVLAIITTAVSLLRGIPVLLEGKQFFVKDKA"
     gene            10260..10907
                     /locus_tag="PK754_06280"
     CDS             10260..10907
                     /locus_tag="PK754_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HNB09144.1"
                     /translation="MSRRIAFFDIDGTITRHSSERLFIRYLLQKKIISYSDLVLATLR
                     YSCKHPTYLKNGFKQNKMYLCGLDAEMIRQHASQCFDEYIRPSIKPIMTEALVMCRKD
                     GYTIVLLSGSLRCLAEPMQRFTQAEDLICSETEIINNRYTGEMSSLHPYGENKKTLAI
                     RYCTDHQIAVRNCRAYANEWADRFLMEAVGEAVAVDSDEKLKRLAIEKKWSVLKT"
     gene            10909..11718
                     /locus_tag="PK754_06285"
     CDS             10909..11718
                     /locus_tag="PK754_06285"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HNB09145.1"
                     /translation="MSKSFFLKQAAWFKDSIREFSVPDEFEVDIINPNDAPPIDNKAI
                     AYAIDHPFGASLNERLVGKCRVVFVVDDLGRPTPAHDIIPVIIPYLTKVGIKDKDISF
                     VIATGSHRQLSIDEMKLKLGSTVAEKYSVHCHDAFNSKLVHLGTLENGFPCIANKIVA
                     EADIIIGIGCVIPHACHGFGGGAKLFCPGIAGMESIVTMHGFTPKRGRVVMDIPEGEW
                     DMRSASEAFAEKLPPIFLINVVMNSQREMCGIFAGNINDTFRAARTMAAQV"
     gene            11773..>12038
                     /locus_tag="PK754_06290"
     CDS             11773..>12038
                     /locus_tag="PK754_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09146.1"
                     /translation="MYPLDSDPVQSGKSPWIKKFFKNAVAIQINSSADGIDYHGWKLL
                     RRSRITTLIILLIGEGLSLKFMPSIFRKPASWSVLKKLRSYCVR"
BASE COUNT         3435 a         2224 c         2707 g         3672 t
ORIGIN      
        1 gttgtcgctc cacagccgtg ttggcttttt tgcctggtcg ctggattggc acaatccatg
       61 ggggcggtat attgcgctca gatgagccac gaaatttttt tgtaaaacta tcgtcgtggc
      121 tcgggggctt atttattcga cgcgctgatg ctattgttac gattagtaac gaagccgaaa
      181 aattgatacg taattctgtg tccgataaaa tattgcaatc caaactgaaa gtcatataca
      241 acggaataga atttaatccg gcggttgtga aaacaaaaga ggacgcccta acgttttgcc
      301 cgcaatggcg caatcgtaaa atagtattga cggttggccg tcttgtggag gccaaaggac
      361 atgatttggt aattcaatct attcatcgtt tgcgaagcga aatccccgag attctttata
      421 tcatagtcgg tgaaggaaat actcgcaaaa atcttgaaat gctggtcaaa aaattaggtt
      481 tggaaaatta tgtgtttttt gcgggttatg ttgagtcctc acttttagaa aattattatg
      541 cagtggcaga tatttttact atgccgggtc gagtgacacg tgaatttgtt gaaggcttcg
      601 gattagtttt tgtcgaagcc ggtatgcgcg gtaaagcggt tatcggtaca agggtcgggg
      661 gaattactga ggccattcga gacaattaca gtggtttact tatcgcgcca gacgatgtgg
      721 agaatctgag cgaggccatg aaaaaacttc tgatctgtcc tgcaacagcc gccgttttgg
      781 ggagacaagg gcgtgaatgg acgataaata atttctcctt ggatgtgatg gcgcgtcaca
      841 atgataggtt gttacttgaa atttattggc gcaaaaaggg aagaaatagt ctaccgacca
      901 atctgatttc taccaacgcg atggaatttt ctaaatagct gtttttttat tgtgcctgat
      961 caatctctat gatttaatct taatttgttg ttgttttggg taaccgagag caccccaact
     1021 caatatcatc ggctgttaaa tttagtacgt tatccacaat tacgctgata gggtattgca
     1081 ttaaatgcgg tctctcataa aaccggatta ttcttcatta ctagaattac atcaatggct
     1141 atcgttaatc gcgaaatatt tttttataat aaggttatat aactttgcag catgttgagt
     1201 tctgttctct cttttcaaat acttcccatt cttcttcttc gcagccccaa taccgtctgt
     1261 ttctttttaa taacccgata agtgtgtctt aaatcacaca aatacatcta tttgaacttc
     1321 gaaatgcaag ttttttattg cacctgcaag ttccttattt ctcgtacatg cagcaattcg
     1381 ataataattt ttaaaaacta attaacaata ttaaagtaaa ttaataaaaa acaacacgtc
     1441 atagtaactt tctttttttt aatatttcgg cacgcagatt gctacataaa tgaatgtcac
     1501 tgtgaatgaa gtggcgttta aaaagtagaa aataagagaa ctggagatat caaatgaaag
     1561 cattcatctt agcggcaggt caaggcaagc ggttgcttcc tcttacggct gagacaccta
     1621 aaacgttggt agatgtcaaa ggcacaacga tactggaata ccagttggtt acgttggcca
     1681 gctgcggtgt caaagatgtt gtgatcatcg gcgggtttga gatcgaaaaa ttgaaaaact
     1741 atgcagagcg ttatattgcg attcatggtt tggatatccg cctcaaaatg gttaataaca
     1801 aagattatgc tactaccaat aatctttttt cactttggtt agctaaagat gaaatgacaa
     1861 ctgaatttgt cgtgatcaat ggtgacaatg tgtttgagcg taaggcatta gcaaaagtga
     1921 ttgcgcaaaa agatatgaat gtcagtatcg ccattcacaa gaatccggct tacgacaatg
     1981 aagatatgaa agtgcgtatc aatggtgatc gtgttattga gatcagtaaa gagattgata
     2041 actacgctgc taacggtgaa tctatcgggc ttcgcgcatt tcgcaattcc ggtatagagg
     2101 cgttcaagca ggcactggat tcggccatga tcaaagatgt tacccgtaat gcattttttg
     2161 tgcgcgctat acagcatatg attgatatgg gtgaaaatgt aggttgggtg gatgtaacag
     2221 atttcaaata cggtgaactg gatttctttg aagatctgca tcacttggaa atcgaaatgt
     2281 ccggcctgat gaagcaaacg attatgttac acacaaatcc tgcgggattg tatgcgcgta
     2341 ccaatggttt gtatcaaccg ttgatgatga aaaacgtcgg ctaatattta gttgtatcat
     2401 aaatgaatca aaatcttggc agagtgtaaa tcctgccaag attttttttt gcaaaaggta
     2461 ttcagggaat attatccatt atatggatac gtaataacaa tacgctctat gtcttttcag
     2521 aacgacgatc acacattatt tagtatagct tttattgcgg gggtgcctat tcccagcaac
     2581 gatgtggtct cacaacaatt tattaacaat gtggcggcaa taatgagcgt caatccgtcg
     2641 tggcgtgtta aagtatttgc agaaaaaccg accgttgggt tcgaaaatgt gaatgtacaa
     2701 gaacattacg gtattgcgca ggacattcct atcgaatata taactgtgcc cacgctgccg
     2761 aagaaatggc gaaaacacta cagcgcctgt aaggcaattt tggccgcggg ccgagaaaaa
     2821 tttgacttct attttacccg tcacccggaa accctgttat ggagtttact agctggtcac
     2881 aaaaccttct ttgacacata ccggattaac ctcaaccaac gtgcgaagta cctgttgtgg
     2941 agaaaatact gttatacgag gccccatttt tgtggcgtga tagcccactc caagttgtgt
     3001 gccgaatcgt ttctaaatgt ttgtctggca tcatccaaaa ttacaattgc ctataatggt
     3061 agtcatccgg gtcggcagat ggatggaaat gaaacaatgg tacttcgaag gcaattgaat
     3121 attgatgatg ggaaactggt gatctgttac gccggacgta ttagtgacag ttccaaagga
     3181 agtggtatgc ttttggattt agcacaacag ctacccaata tcatatttct ttttgtaggt
     3241 ggcggaaaaa aagaaagaga tgaatcagat tttgataaca aaatacgtac gcttcgatta
     3301 tccaacgtgc ggcgaaccgg ttgggtggaa ccttcggcgg tgagtcggta tttacaactt
     3361 gcagatatcc tgcttattcc ccctacagca gcgggtctcg aaaaaaaagg caacacggtt
     3421 ttgccaatta aaacctttca gtatcttcaa gccgggcgtg ccatcgtagc tccagacttg
     3481 ccggattgcc gcgagatctt acagaataac caaaacgcta tattggtgcg acccgatgac
     3541 cttgacgcag ctagaaaagc ggttcaagta ttgtcagaga attccgattt gcgcatatcg
     3601 ttaagcagaa atgcatatag ggatggtctc aaatacacgt ggactgaacg tgcaaaattt
     3661 atcagcgctt ttattcgtac tcgctcggcg tcataacgaa cgtgcttgaa gagtgtgatt
     3721 acagtagtaa tatttagatg tttcggattg ttttttgctt taaggtatca catgttttgt
     3781 atatgttaaa tgaccgcttt actactaata ttaatacgat agattttatt gtaatgaagc
     3841 tgttttataa acattacgta tattgatttg cagatggctc agatttagcg gtctcttgtg
     3901 tacaaaagaa aagcgtatga aaacaaaaag ataatataat catagtcatc aataagcact
     3961 tagtttaagt ggtcattaac taattaacac tggtacgcat tccatgaata atccaccgcg
     4021 gatagcagtt tttaaaacca agtttctacc ttattctgaa accttcattc acgatgagtt
     4081 acatgctcat caacgttaca aaccgtttgt tttggctaag aaacacttaa acgctgctca
     4141 atttactgat catgaagtta tcaatttttt gccttcagat aatctcttaa atcgtattga
     4201 acattacctg tttaatcggt ggcgatggag ttggacattc aaacaagttc ttataaggaa
     4261 ccgtgtttgc ctgatccacg cgcattttgc gtacagcggt atttttatgg tgcctttggc
     4321 aaagaaattg aatatacctt tgatcgttac tttgcatgga aatgatgtcg caatactgct
     4381 gggtgatcaa ctacatcaaa aaaaatgggc tttatataaa aaaaaatata aagaattact
     4441 tcatcaagca agtttgtttt tggcagattc tcaagaactt aaagatcgta ttgttgaaat
     4501 cggatgcccg gctgaaaaag taattgttca tacattaggt atagatttaa aaaaatttcc
     4561 ataccaagca caaaaaccat ctttgccgcc aaggatactg atggttggac gtttcgtgga
     4621 aaaaaaggga tttatatacg gtattcgagc ttttgcagca gcacgaaagg atataaagaa
     4681 tgcggagttg catattatcg gggatggcga gcttcgcaca gaatatgaag cggaagcacg
     4741 gtctttggga gttgcatcga ctgttttttt tcatggtgtc aggccgcacg aagaaatagt
     4801 gcagcatatg catcaggctg cattgttttt agctcctagc gtcattgcgg caaacaaaga
     4861 tagggattct ggattgattg tagcaaaaga agcagcagct tgtgggttac cggttatagg
     4921 ctctatccat ggtgggatac cagatattat tgcagacggt gaaacaggtt ttctaattcc
     4981 tgaacgtgat gtaataaaaa tgagccaatg tatagtagaa attttgaata accctgaatt
     5041 atggagtcgg atgagtatag cgtctaggaa aaaaattgaa gaagaatata atattgagaa
     5101 atgcaatgag cggtcatcat ctatttatga tgaattatta aaatctatgc aggccatgta
     5161 attattgcaa cgcttataca acatcagtca attgaaattg gtaattgaaa ttaaagaaaa
     5221 aaaatccaaa tcgtatagga taagaaaact gatcaaacga ctattcttga tacaatgtta
     5281 aatagttgcc atattgctta tacggaatcg agactacact atgagacaag atatatcgtc
     5341 gcgcataaaa gaatccatta caaccaaaga aaaaattctt tcggatgagg tattgatcgc
     5401 aaacatcgaa aaagtaaccg atattatcat atcggctttt cgctcaggtc atcggttgta
     5461 tctttgtgga aacggcggca gcgcggcaga cgcacaacat attgcagccg aattatccgg
     5521 acgcttttat tatgaccgca aaccattgta tgcggaagca ctccatgtca attcgtctta
     5581 tgtgacagcg gttgccaatg actacggata cgatgtagtg tatgaacgta tggtggatgc
     5641 cgcagggcag gctggtgatg ttttgatagg aatctccact tctgggaatt ctcctaatgt
     5701 ggttaaggca ttacaaaagg caaaagtgct aaatatgcat accgttggaa tgacaggagc
     5761 aaacggcggt gtcatgaaag gtatttgtga tattttgatc aatattcctt ccaaagatac
     5821 accccgaatt caagagtgtc atatattagt cggccatatt ctgtgtgaat tgatcgaaca
     5881 aacacttttt ccaaaaccca aatgatagct atagtagatg cgcgaaatac ggcgtccaga
     5941 cgctgtttgt atagtatatc catgctagaa cgcttattgc gtcaactcca tgaaaacaac
     6001 gttactgaag cacggattct gatttctgaa gatcagtctc atcacctttc gtttcggcgt
     6061 gagttttcgt ctcgttatgt tatgcatctg acttatattc ctacgcgtga aacaattaca
     6121 cagtatttat cgaaggccaa gatttcgtcg gatgtacttc tactgaatgc tcacgtattg
     6181 cacgatctgc gaatcataaa aaaactagta gattcgacca attcgttgat aattgttgat
     6241 gagaatcatt cgggcccatg tgctgctaaa tttattcatg atatttcgat tgtgtacgat
     6301 aattcagggc aagacattga actgcgaaaa acacttctta tactagctca gcagaaaagc
     6361 atagaagtgc tacgagtttc tgaaatgcaa tcttatgttc ggtttttacg tcgtcatgct
     6421 acgccgttat ttcaaagcat tacgccatcc accgatatga atgcactgga gcatataatg
     6481 tatgaagaca ccttcaaagg aggtatggag tggattgctg tatatggata taagataccg
     6541 gtgcgggaac ttacaaaatg gtgcgcccga acttcatgga taacacccaa tgtcattaca
     6601 gctttagcta caataggaaa attggtggcg attcctttct tttttatagg ttggatcggt
     6661 tgggctttgt taagtttttg gatctttacc ataggagatt cgctcgacgg aaaactggca
     6721 cgtatgaccg ttcgttttag taaaacagcc gaccgagtgg accatctgac tagtgcaccg
     6781 gcccgcatgc tttggcattt tggtttgacg tggtatatgt gcggtggtaa tctcacctct
     6841 cctataggta tggccggtat tggatttagt ctcttgccac agattgacaa gtttggattg
     6901 atgtatttca attggaaatt cggacgttca ccgattgatt ttacatcgtt tgatgctagg
     6961 gttcaccttt atacagctcg acgaaatgac atggtgttgc ttacgatcgg atacttagca
     7021 ggatttttac tccctgcttt ttacggaatg atggcttgga tgtatctgac atggttatgg
     7081 catgtcgtgc gtatttcatg gttttcattt atcaatcccg tcacgcgcgg atctgttttt
     7141 aaaaagcata ccacccattt atgagagttt tcattctggc aatgaagtat cttccagcga
     7201 gcggaggtac tgcaacctat gcacataatc ttgcacaagg ccttgcccgt atgggacatg
     7261 atgtattatt ggtatctcct gactatgcgc gaccccagtc ggatgacgga ttaccgtatc
     7321 gagtaatacg tttatcccaa gcaagacttg ggctaggtca attccgaatt tttctaacgg
     7381 cttatcatac aaagaagatt ctaaataatt ttaatccgga tgtgatttgg gcatctacat
     7441 atgccggttg tcgtgtgctt gggttactta gattgcgaat accgaccgta gggactattc
     7501 atggcggagg cattcaacgg gctttttcat cacttaatcc gatcaaacgt tgtacagatc
     7561 atgcaggatg gcgttttatg cgacgtgcgt ctgcgatgat cactgtcagt gaagaagcaa
     7621 agaaattagt ttgttcggcc atccaggata cactgataca aagtaaattt cgggttattt
     7681 ataatggtat agattttgat ccttcttcgg tggtatccaa aaaagaagcg ctctcacgct
     7741 ggccacaatt tgaagggcgc attattttgc ttaccgtcgg gcgattagtt aaagccaaag
     7801 ggcacgatgt ggtaatccgt gcacttccag aaattcaaaa aagagttcct aatattttat
     7861 atgtcattgc cggtgaaggt gtcgaaaaag aacaactcat taatttagtc gaatcgttaa
     7921 gtttgaaaaa tagtgttcat tttacgggat atattacacc tactggtctc gaagagcttt
     7981 atgcaatcag tgacttgttt gtgttggctg gtcgctggac tccgactttt gtcgaaggat
     8041 tcgggcttgt gctggttgaa gcggctattc gtgggaaacc ggtcatcggt gcacgtatag
     8101 gcggaatacc cgaagccatt gaagacggcg tttccgggat agtgatagaa cccgaagatc
     8161 cttctcaagt tgcaaaagcg gtgatggata ttctgagtga ccaaaatttg tatgatcgaa
     8221 tggcgaaagc aggccctgtg tgggttaacc tgcattttac acaagctatc atggcacgaa
     8281 atatcgatca actgctttgt tacataaccg gtaaatcctg atggccagtt tattagttca
     8341 atcaggtcgc atattacttg gtcgtgccag catttcggtt atctcaattt tttttacaat
     8401 ctactacgct aggacactgt cacgtactca gtttgctttt attgttcttt atgaaaccgc
     8461 cgctgcattg tttatgtctc ttactgaatt aggcatgtat tcgaaagtag ttcgagaagc
     8521 gcccccgctg ttgcgagacc acaaagagtc gttcgtcata gatcacatta ttatgcctgc
     8581 aacagtaacc cgggcgatgt ttggtttttt tctttcactg ttgtatggta tgacggcgtg
     8641 gatatttgaa gctgaacttc ctgctgaact tcgtgcgctc aacatggctt gggtcatcgt
     8701 atttgcttgc ataggtattt tttttgaagg attgggaact ataacggaag ctattttcgc
     8761 ggtcaaacag cgtttcggaa tgaattcctt tttgggtagc gcacagactt tggttgaaca
     8821 aatatttgca cttttgtttt ttgtacaatt tggtttgacg tattattttg taggtgtaag
     8881 tattgcgcaa gcagtgatgt ttttattgcg aatttggtat atcagggatg ttgtatttca
     8941 atttcgacca aatttctatc gttggaattc tgctaaaaaa atgcttaaag aatactttcc
     9001 attttatgtg cgtcggtttt tcaggttagg ttttttacag ggcgaacaga tcgctgtggc
     9061 gtttcttttg ccgctagaac aattagctaa ttttaatctg gccaaacgca cggctaaatt
     9121 tatgaaaccg tatattgaag cttttttaaa tcctttaatc atcaaattgt ccaaagcgcg
     9181 cgatattaca ttaatgaaag aataccgaaa gacttttttg tggtttacaa tgccggtacc
     9241 tgttatccta gccttagcga gcccgtggat tatacagata tacggtgaaa aatatgccgc
     9301 cagttggcca ataatggcaa tttttagttt atcgtggatt ttttatgcac tttcgacctt
     9361 gcaacttgga gttgttggaa tttttggaga gccgaaagag gcgttgatgg cggatgcttt
     9421 cgcgggcatc ttcggcttga gcactactat tcttttgatt ttgctgttta aagaatatgg
     9481 acttgcgtgg gggcaagtga tttcatttgc cacattgtat atttccggat tgtatatcgc
     9541 tcggaagtat tatcgcaacc cgcaactcat caagcctgta accgtgcaac ccatcacgca
     9601 ggaaaaccca tcatgatacg attgttgaac ctcgctaatt ccattaccat tgcgcgcatc
     9661 ggcgttttat acataacggt tgtattgatc tatacgcagc gcccgatgtg gcttatcatt
     9721 gctgtcctgt tagctatcct gatcattgtt atggacgcac tagacggtta tgttgcgcgt
     9781 aagcgcaacg aagtgactca gtttggcgga gttcttgata ttaccggtga ccgtattgtc
     9841 gaaaatgttt tttggattgt tttcgccgat ctggacatca taccaatgtg gatacctatc
     9901 attgtgatgt cgcgcggttt tatgacggat gcgattcgca gccaagcact agccgatggg
     9961 aaaacggcat ttggcgagaa taccatgatg caatctgaga taggaaagtt tttagtatcc
    10021 gggcgcttca tgcgtgcttt ttacggagta attaaagcga tcactttccc atatctgatt
    10081 cttgttttat tagccaagga acagcatttg cgcgatgcta atctacagga ttatttgtgg
    10141 ttatcatcgt tgacgtacaa tggtgggttg gtgttggcta ttatcactac ggccgtgagt
    10201 cttctgcggg gtatcccagt tttacttgag gggaagcagt tttttgtaaa ggataaagcg
    10261 tgagccgtcg tatagctttt tttgatattg acggtacgat cacgcgtcac tcatccgagc
    10321 ggcttttcat tcgctatttg cttcaaaaaa aaattatatc gtattccgat ctggtattgg
    10381 ctacgctgcg gtattcgtgc aaacatccga cgtatctaaa aaatggattt aaacaaaata
    10441 aaatgtattt atgcggatta gatgctgaaa tgattcggca gcatgcttca caatgttttg
    10501 atgaatacat ccgtccgtca atcaagccaa taatgacgga ggcgcttgta atgtgccgca
    10561 aggatgggta tacgattgtt ttgctcagtg gctcattgcg gtgtttagcc gagccgatgc
    10621 aacgttttac gcaagccgaa gatttgattt gttcggaaac agaaatcata aacaaccgat
    10681 ataccggcga aatgagttca ctgcatccgt acggcgaaaa caaaaagaca ttagcgatac
    10741 gttattgtac agatcatcaa atcgctgtga ggaactgccg ggcctatgcc aatgagtggg
    10801 cggatcgttt tcttatggag gctgtcggag aagcagtggc cgtggattcg gatgaaaaac
    10861 tgaaacgact agctattgaa aaaaaatgga gtgtattaaa aacgtaatat gagcaaatcc
    10921 ttttttttga aacaggcggc ttggttcaaa gattctatca gagaattttc tgtacctgat
    10981 gaatttgagg ttgatattat caatccgaat gacgcgccac ctattgataa caaggccatt
    11041 gcgtatgcca ttgaccatcc tttcggtgcg tcactgaatg agcggcttgt gggaaagtgt
    11101 cgggtagttt ttgtagtaga tgatttggga aggcctaccc cggcgcatga catcattcct
    11161 gtgataatcc catatcttac aaaagtcggc ataaaggata aagatattag ttttgttatc
    11221 gcaacgggct cccatcggca actatcaatt gatgaaatga aattaaaatt ggggagtacg
    11281 gttgccgaaa aatattcagt tcattgtcat gatgctttca attctaaact ggttcaccta
    11341 gggacattgg agaacggctt tccttgtatc gctaataaaa tagtagccga agcagatatt
    11401 attatcggga ttgggtgcgt gataccgcat gcctgtcacg gttttggggg tggagctaaa
    11461 ctcttttgcc cgggtattgc aggtatggaa agtatagtga ccatgcacgg gtttaccccc
    11521 aaacgcggca gagtagtgat ggatataccg gagggtgaat gggatatgcg ttcagcttcc
    11581 gaagcgtttg ctgaaaaact acctcccatt tttttgatca atgtcgtcat gaattctcaa
    11641 agggaaatgt gtggtatttt tgctggaaat ataaatgata cttttcgcgc ggccagaacc
    11701 atggcggcac aggtataacg aactgaaata gatccgaaaa aagctcaaca atatgatgtt
    11761 gtatttgtaa atatgtatcc gctcgattca gatccggtac aatccggaaa aagcccatgg
    11821 ataaaaaagt tttttaaaaa tgctgtggct attcaaataa acagttcagc agacggaatt
    11881 gattatcatg gttggaaatt gcttcgccgt agtcggatta ccaccttaat catattattg
    11941 atcggtgaag gactcagttt aaaatttatg ccatccattt ttcgtaagcc ggcatcttgg
    12001 agtgtattaa aaaaactgcg atcgtattgc gttagaaa
//
LOCUS       DAOQNQ010000070        11891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1913_length_11891_cov_5.338205, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000070 DAOQNQ010000000
VERSION     DAOQNQ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11891
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1913_length_11891_cov_5.338205"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="PK754_06295"
     CDS             complement(<1..577)
                     /locus_tag="PK754_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09147.1"
                     /translation="MSQEGLSREFKVNLIKSVYSKTAFDEVIAKANPYQLSDIQEFMW
                     DAALYIGRDEAGKPITREMITSRMMPTTEYWRGQLCGEPIDTCRGRSCFVSHPVCAGN
                     KLRAQIEVIRSCFDRRKQRSQQNEEGILMLLMYHINENHYAAAGLYDIQKHPIKTVGS
                     RVLSGDSLRNVIQAMNVAVGQGFTDSHMVQPV"
     gene            complement(612..1646)
                     /locus_tag="PK754_06300"
     CDS             complement(612..1646)
                     /locus_tag="PK754_06300"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011936954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNB09148.1"
                     /translation="MNTKEFMDTKEIIEVGVIGGGSFGTAMAIHLARNGYPVTIWAHD
                     AQAAEYMQTQRENKTYLPGVVLPNSLTATSDLQQTVRGKKLVLQVNPSHATRDIMQRA
                     VPFLEDDAIILNCTKGIENESLLTMSELLLQIIPSSKHSQLAFLSGPSFAREVALGAP
                     TAVTISSKNPSSAQYCQKIFASERFRVYTTDDIVGSELASALKNVIAIAAGISDGLGY
                     GHNTRAALITRGVAEILRLGKKMGAKEMTFLGLSGMGDLVLTCTGDLSRNRSVGIQLG
                     QGKKLEEILASMKMVAEGVKTAKAAYALAQKHGIEMPIIEQVYRVIFENKDPRAAVYD
                     LMTRESKSEA"
     gene            1789..2346
                     /locus_tag="PK754_06305"
     CDS             1789..2346
                     /locus_tag="PK754_06305"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNB09149.1"
                     /translation="MKIYLEAGVGFQSGTDVYGNEITGSSNTELTVKYLQEYLNNQSE
                     IRDRKTEVITTSNAATVLETMQPEDIFLTVHLNYYRQPQGVGLRGFYRSSRKLNSEKC
                     KALAITVTKALHDHTDVYYHGVFNEQRNNKPQLEPVLSTKATAALVEVGFNDDFKTKS
                     QSEMLHKNLAEGLGKGLVRHINAIR"
     gene            2574..3764
                     /locus_tag="PK754_06310"
     CDS             2574..3764
                     /locus_tag="PK754_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09150.1"
                     /translation="MLRKKLIWLSMLMATSAMVSAQIGNGLGTGRMGTFNTYSASVMG
                     AGFLSTYNQGRLFAVAGQSGFGTIKNVNLYHASNNFTAAYGFMDNFDALVSIITYQDI
                     NIRTVRSSQSLVPGDLYLSIRTGSYQIGEGSLEYGGSVTGRFPTGGQHNIPFEVYRSK
                     NIEFGVMGLLSYYSNPYYKDQSYSLNLNLGFWSHNDNGTVVTPSDDPNYSKAESDVNA
                     LALQYGLGLQIPVSKVHFMLDLYGLNYLTAPTKYVFSRESFAYSSFGLRYNLRSWLNV
                     GTFFDLQLAGKSDKTSYDGVGKLYGVTKPGQPANSSSKGAPNYNTWRFGFGLGFNILP
                     ATFGNSGGAVGGEQRRKKLLDRLMEEEKGAQRASNQLDKLKNIRVNAEKELEQIRLEL
                     EGGQ"
     gene            3777..4115
                     /locus_tag="PK754_06315"
     CDS             3777..4115
                     /locus_tag="PK754_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013571272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNB09151.1"
                     /translation="MKKIEAIIRPFKLDDVKEGLLQVGVKGMTISEVRGFGRQKGHTE
                     LYRGNEYRIDFLPKIKLEIVVENAMLDTTIDMIIDRAKTDQVGDGKIFVTNLEEVIRI
                     RTGESGEEAI"
     gene            4275..5696
                     /gene="glnA"
                     /locus_tag="PK754_06320"
     CDS             4275..5696
                     /gene="glnA"
                     /locus_tag="PK754_06320"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNB09152.1"
                     /translation="MSKEAIKKAMDLAKNRNVQVVDLKFMDFIGVWQHFTIPIYELND
                     EVWEQGLGFDGSSIRGWKAINESDMLVIPDPNTAMIDPFQQPTTLSIVCDVHEPLTKE
                     RFSRCPRGIAHAAEAYLKSTGIADTAYFGPEAEFFIFDSIRFDQTQNAGYYYIDSIEG
                     VWNRGRDENPNLGYKIRNKEGYFPVPPADQFQNMRNEMIMIMHECGLHVERQHHEVAT
                     AGQAEIDYKFNSLVRSGDDLMLFKYIVKNVAWKYGKTATFMPKPIFGDNGSGMHVHMS
                     LWKAGQPLFAGNQYAGLSELALYFIGGILKHTKALNALANPTTNSYKRLTPGFEAPVN
                     LAYSQSNRSAAVRIPMYSNSPKAKRVEYRCPDPSCNPYLAFAAMMMAGLDGILNKIHP
                     GNPLDKNIYDLAPDELAKVPSTCASLDEALRALENDHDFLLKGDVFTEDVIKSWISYK
                     YENEVNPMRLRPHPYEFALYYDM"
     gene            5818..6928
                     /gene="prfB"
                     /locus_tag="PK754_06325"
     CDS             join(5818..5892,5894..6928)
                     /gene="prfB"
                     /locus_tag="PK754_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_121874281.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNB09153.1"
                     /translation="MIEISDIEKKLELAQFKISELRRFLDVDQRDQKIANLEAQTTVE
                     DFWNDNQRAQQIMQQIASERAWVDQWKKLDAQCEDLNMLLEIAEEENDEATIKSIDTE
                     LTELEKALARLELRAYFTNPEDNKSTVLTIHPGAGGTESQDWAEMLMRMYQRWAADRG
                     MKCQILDILDGDGAGIKSVTMEISGEFAFGLLKCESGVHRLVRISPFDSNARRHTSFA
                     SVYAYPEMEEVEAKIEINPADIKMDTYRSGGKGGQNVNKVETAVRLTHIPSGIVVACQ
                     VERSQHKNRDLAMKLLISKLYQMRKKEEQDKLDKLESAKSDIAWGNQIRSYVFHPYNL
                     VKDHRTDTETSNVQAVMDGDIDMFIEACLKQKVVQ"
     gene            6935..8632
                     /gene="argS"
                     /locus_tag="PK754_06330"
     CDS             6935..8632
                     /gene="argS"
                     /locus_tag="PK754_06330"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNB09154.1"
                     /translation="MKSYLEQQLHSALKALAIASDSEIIFTKTKDERFGNVATNLAMN
                     LAKTQKMNPRELAQKIVDHLQLDSSLVTKAEVAGGGFINFFFSPNYLFTQLKTILLQG
                     EEYGRHRFGANQRVDVEFVSANPTGPLSIGHGRQAVLGDVLCRLLSNAGYQVTREYYF
                     NNAGVQMKKLGESVRLRYLELLGETITFPEKHYEGEYIRDIAADIKSQHGESWKSYDF
                     EPFKSFAEDILFANIRKVLGRIGLKFDVYYNEDSLYKEGKIEALLKSFAAKNLTYVKE
                     NATWLKTSEFSYDHGPKPENDKVIVKSTGEPTYRLPDMAYHVTKFERGFDHIIDIFGA
                     DHIAEYPDVLAGLKALGYDTSHVRVLIHQFVTLMKEGEILKMSKRKANFVTIDELIDL
                     LGPDVFRYFLIMRSHNSHLNFDLELAQKQSMENPAYYIQNAHARICSIEKKAGERDIL
                     FSVSNEIKLDLLKADEETTIIEKLLQFPELTARLAKNLEPHPLTTYLEELAGVYHHYQ
                     TAGKKNPDLRVITEDKALSEARLALCIAVRIVLRRGLDILGIHAPEYMARDQADETV"
     gene            8704..9612
                     /locus_tag="PK754_06335"
     CDS             8704..9612
                     /locus_tag="PK754_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNB09155.1"
                     /translation="MDILVVGSVALDSVETPFGKVEDAVGGSATFFSAAASYFAPVNL
                     VAVVGNDYPMHAIEYLKSRRVDFKGLQIEQGETFRWAGRYNYDLNQRDTIYTHLNVFQ
                     NFKPHIPDAYKSSQYVFLGNIAPELQFDVLDQVQSPKLVALDTMNFWIEGSQKALRKV
                     LERIDIFILNDSEARQLANQPNLIKAAKEIFKMGPKAIVIKKGEYGAALITPDAQFNV
                     PAYPLEDVYDPTGAGDTFAGGFMGYIAKTDDASFENLKRAVVYGSAMASFCVQDFSIN
                     ALKELSQDKIFDRVLAFKQLSHFEEK"
     gene            9628..10095
                     /locus_tag="PK754_06340"
     CDS             9628..10095
                     /locus_tag="PK754_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNB09156.1"
                     /translation="MKLSEILNAKLIRIPLLQSNKQDIILEMLDVLVESGKISDREKV
                     LQSVLEREQMMSTGIGNSVAIPHGKSSGVEELQVALGITAQDINFDALDGKPVRIIFM
                     LVGPEKASSLHIKMLSRISRLLNQAPFRKKLTDAQSADEIMQIISDEEKLLEG"
     gene            complement(10092..10391)
                     /gene="yhbY"
                     /locus_tag="PK754_06345"
     CDS             complement(10092..10391)
                     /gene="yhbY"
                     /locus_tag="PK754_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HNB09157.1"
                     /translation="MSELTGKQKRFLRGLGNAIKATISVGKSGITDNTYLSVNNGFNT
                     KELIKVKVQDGCDLDKEEVAEMISEGTQSQIVQIIGNNILLYKCHREKPIITLPD"
     gene            complement(10399..10974)
                     /locus_tag="PK754_06350"
     CDS             complement(10399..10974)
                     /locus_tag="PK754_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09158.1"
                     /translation="MQTESEYNASDAAVSNVKPDSASDSNKKYLFTGLWLIVIGTFWI
                     LWNMSVLSWGWMERYGLIIIGGVSIIKTIVTKKYYVFFGLTLLLIGVFHLYLDTQEGL
                     AMRTLWPVYFLISGIAFVINFFFNMRRWFSLVAGLSLIGFGGIHISRNFYMFPYDWVF
                     IIRTYWPLTFVASGIILVCAAWYKHRKLPND"
     gene            complement(10984..11658)
                     /locus_tag="PK754_06355"
     CDS             complement(10984..11658)
                     /locus_tag="PK754_06355"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNB09159.1"
                     /translation="METTKQLFKSRRDKMVDGVCAGLAEYFNVNVALVRLLFVVFAFV
                     DCISVVAYITAMVLVPREPVSLTQDPDMELIEKRSAVNHGFFWGAILILIGAIMFLDT
                     MNLFNFHFFWRHFGWKFLVPTVFIIAGIFLIASRNRFAGSAGFFNKTSESTFRRASEN
                     KKIFGVCAGAAKHWNLDVSIVRIAWVLITIIAFPVGLIAYALVGLLTPDDKDQKVFGA
                     ASEKKL"
BASE COUNT         3532 a         2583 c         2455 g         3321 t
ORIGIN      
        1 taacaggctg aaccatatgg gaatcggtaa atccctgccc tacagctaca ttcattgctt
       61 ggatgacatt acgaagcgaa tcgcccgaca acacacggga cccgactgtt ttgattggat
      121 gcttttgaat gtcatacaat ccggcggccg cataatgatt ttcattaata tgatacatca
      181 gtagcatcag aattccttct tcattttgtt gcgagcgctg tttacggcga tcaaaacaac
      241 tgcggataac ttcaatctga gcgcgtagtt tattgcctgc acataccgga tgtgaaacaa
      301 agcatgatcg cccgcgacaa gtatcaatgg gttcaccgca aagttggccg cgccaatact
      361 ccgtggtcgg catcattcgc gatgtgatca tttcgcgtgt tattggctta cccgcttcgt
      421 cacgcccgat gtaaagcgct gcatcccaca taaactcctg aatgtcagat aattgatacg
      481 ggttagcctt ggcgataacc tcatcaaagg ctgttttact ataaactgat tttatcagat
      541 ttaccttaaa ttcgcgtgat aaaccttcct gtgacatatt gatttcctta tttctgcctc
      601 gttaaagagg ttcaggcttc cgatttggat tcgcgtgtca tcagatcata taccgctgcg
      661 cgcggatctt tattttcaaa tataacgcgg tacacctgct ctatgatggg catttctatg
      721 ccgtgttttt gtgctaacgc ataagccgct ttggctgtct taacgccttc ggcaaccatt
      781 ttcatggagg ctaaaatttc ttcgagtttt tttccttgtc ctaattgaat gccgacgctg
      841 cgatttcgac tcagatcacc ggtgcatgtg agaaccaagt ctcccatacc cgaaagaccc
      901 aaaaaagtca tctctttggc tcccattttt ttcccaagac gcaatatctc cgcaacacct
      961 cgcgttatga gcgccgcacg agtattgtgc ccataaccaa gcccatcgct gatacccgcc
     1021 gcaatggcga taacattctt cagcgcactc gccagttcag atccgacaat atcatccgtc
     1081 gtgtatacac gaaaccgttc actggcaaaa attttctgac aatattgcgc cgaggaagga
     1141 ttcttagaag aaatcgtaac agccgtcggc gctccgagcg ccacttcccg cgcaaaactc
     1201 ggtcccgata aaaaggccag ttgactgtgt tttgaagacg gaataatctg aagcaataat
     1261 tctgacatgg ttaacaaaga ttcattttca attcctttgg tacaattgag aatgatcgca
     1321 tcgtcttcca aaaaaggaac ggcacgttgc atgatgtctc tcgttgcatg ggatggatta
     1381 acctgcaaaa ccagcttttt accacgcaca gtttgctgta gatcagatgt agcggtcagg
     1441 ctattcggta aaacgacacc cggtaaatag gttttgtttt cccgttgggt ctgcatgtat
     1501 tcagcggctt gtgcatcatg cgcccaaatt gtaacagggt aaccgttacg tgccaaatga
     1561 atagccatgg ctgtcccaaa acttccaccg ccaatcacgc cgacttctat tatttctttt
     1621 gtatccataa attcttttgt attcataaag aaaatatagt atacgttgct tattatatca
     1681 agacttatta aaacacaaat tttacaataa gttagtttta acttgaattt ttattgtatc
     1741 ggtaatactt ttcccttgtt caagcacttt tcttaggaga aatattcgat gaaaatttat
     1801 ctcgaagccg gagtcggctt ccaatccgga acagacgtgt atggtaatga aattaccggt
     1861 tcatctaaca cagagttaac cgtaaaatat ttacaagaat atttaaataa ccaatctgaa
     1921 atacgggatc gtaaaacgga agtaataacc acctccaacg ccgccaccgt acttgaaacg
     1981 atgcaaccgg aagatatttt tttaacggta catctaaatt actacagaca gccccagggt
     2041 gtgggcttac gcgggtttta tcgaagctcc cgtaagctca attccgaaaa gtgcaaagcg
     2101 ctggctatta cagttaccaa agcattgcat gatcacacgg atgtatacta tcacggtgtc
     2161 ttcaatgagc aacgtaataa caagcctcag ctcgaaccgg ttttgtctac taaagctaca
     2221 gccgctttgg tagaagtcgg ttttaacgat gattttaaaa caaaatcaca gtctgaaatg
     2281 ttacataaaa atttagccga aggattaggt aaaggtttag ttcgtcacat aaacgctata
     2341 cgctaaaagt gaccattaag accaaaaaac tgtattattt tattggtata attacaattg
     2401 aataggtcac tttcccttga aaaaagatga cttaaagttc attttaataa atacttgaca
     2461 ttgcaccaat aatcatctat tttttctcaa aataccgtca tattgcttaa tttaagaatt
     2521 atacagatta atattaaaat ttatttataa acatccgaaa ttcggaggaa tgtatgttgc
     2581 gtaaaaaatt gatttggctg agtatgttaa tggcaacttc agccatggtt tccgcgcaga
     2641 tcggcaatgg gcttggaacc gggcgtatgg gtacgtttaa cacctattcc gcatctgtca
     2701 tgggtgccgg cttcttgagt acttacaatc agggacgttt attcgcagtg gcaggacaga
     2761 gcggatttgg aacaatcaaa aatgttaatt tatatcatgc gtccaacaat ttcacagcag
     2821 catacggatt catggataat tttgatgctt tagtttctat tattacatat caagacatca
     2881 atattcgtac tgttcgcagt tcgcagtcat tggtaccagg cgatttatat ctctcgattc
     2941 gaactggaag ttatcaaatt ggtgaaggta gtttggaata tggcggatcc gtcacaggac
     3001 gttttccaac cggaggacag cataatattc cttttgaagt atatcgttca aagaatattg
     3061 aattcggtgt aatgggatta ttatcgtact atagtaatcc atattacaag gatcagagct
     3121 atagtcttaa tttaaacttg ggattttgga gtcacaacga taatggtact gtagttacgc
     3181 catcggatga tccaaattat tcaaaagctg aaagtgacgt caatgctctt gcacttcaat
     3241 atggattagg tttgcaaatc cccgttagta aagtacattt tatgttggat ttgtatggcc
     3301 taaattacct caccgctcct accaaatatg tatttagtcg tgaatcgttt gcttattctt
     3361 ctttcggttt acgttacaat cttcgctcat ggttaaatgt tggaactttc tttgatctcc
     3421 aattagccgg aaaatctgac aagacgagtt atgatggggt tggcaaatta tatggcgtta
     3481 ccaaaccagg tcaacctgca aattcttcct caaaaggtgc acccaactac aatacttggc
     3541 ggtttggctt cggacttggg tttaatatcc ttcctgccac ctttggtaat tcaggtggag
     3601 cggttggtgg tgaacaacgc cgtaaaaagc ttttggatcg ccttatggaa gaagaaaaag
     3661 gcgctcaacg cgcatccaat caacttgata aactgaaaaa catccgggtt aatgccgaaa
     3721 aagaacttga gcaaattcgt ttagaattgg aaggcggaca gtagtatata caggaaatga
     3781 aaaaaatcga agcaatcatt cgaccattta aacttgatga cgtcaaagaa ggacttcttc
     3841 aagtcggtgt aaaaggaatg acgatttccg aagttcgagg atttggaaga caaaaaggac
     3901 acacagagtt atatcgaggt aatgaatatc gcattgattt tttaccaaaa ataaaacttg
     3961 aaattgtagt tgaaaatgct atgttagaca caaccattga catgattata gatcgggcta
     4021 aaactgatca ggtcggcgat ggtaagattt ttgtaaccaa tttagaggaa gtgattcgga
     4081 ttcgtaccgg agaatcaggt gaagaagcga tctaacatac gagccgttcg acggcgaaca
     4141 cgagacatcc gaagtgtctc tatcgccaaa aaaagtacat tataggaatt ttagcccctg
     4201 accaaaaatc aggggctttt gattttaatt aacttaagta agcaacaatt cacttatcaa
     4261 ccaaggagct atcgatgagc aaagaagcca tcaaaaaggc tatggacctt gctaaaaacc
     4321 gcaatgttca agtcgttgat ctcaagttta tggattttat tggtgtgtgg cagcatttca
     4381 ccatccctat ttatgagctg aacgatgaag tttgggaaca aggtctcggt tttgacggtt
     4441 ccagtattcg cggctggaaa gctatcaatg aaagtgatat gcttgttatc cccgatccga
     4501 atacagccat gatcgaccct tttcagcagc cgacaacatt gagtattgtg tgcgatgtgc
     4561 acgaaccgct gaccaaagag cgattcagtc gttgtcctcg cggtatcgct catgctgccg
     4621 aagcttatct caaatccacc ggaatcgccg atacggctta ctttggtccg gaagccgaat
     4681 tttttatttt cgacagtatc cgatttgatc agacacaaaa cgccggatat tattacattg
     4741 actcaattga aggtgtatgg aatcgtggcc gcgatgaaaa tcccaattta ggctacaaga
     4801 ttagaaacaa agaaggttac ttccccgttc ccccggctga tcaatttcaa aatatgcgta
     4861 atgaaatgat aatgatcatg cacgaatgcg ggcttcacgt cgaacgtcaa caccacgaag
     4921 tcgcaacggc cggtcaagca gaaattgatt acaaatttaa cagtctcgtc cgctccggtg
     4981 acgatcttat gcttttcaaa tatatcgtta aaaatgttgc atggaaatac ggtaaaacgg
     5041 ctacgtttat gccaaaacct attttcggcg ataacgggtc gggcatgcac gttcacatga
     5101 gcctctggaa agcagggcaa cctctttttg ccggcaatca atatgcagga ctgagcgagc
     5161 ttgcactgta ctttattggc ggaattttaa aacatacaaa agcgctcaac gccttggcaa
     5221 atcctacgac gaactcctac aagcgcctta caccgggatt tgaagcgccg gtcaatctcg
     5281 cctattcgca aagtaatcgt tcggcggctg tacgcatacc gatgtattcc aatagcccta
     5341 aagcgaaacg tgtagaatac cgttgccccg atccgagttg taatccctat cttgcgtttg
     5401 cagccatgat gatggccggt cttgatggta ttctcaataa aattcatcca ggtaaccctc
     5461 tggataaaaa catctacgac cttgctccgg acgaattggc caaagtacct tcgacttgcg
     5521 catcgttgga tgaagctttg cgagcattag aaaacgatca tgacttcctt ctcaaaggcg
     5581 atgtcttcac tgaagatgtg atcaaatctt ggatcagcta taagtatgaa aacgaagtga
     5641 accccatgcg tctgcgcccg catccgtatg aatttgcttt gtactacgat atgtgatcgt
     5701 ttgaactcta ctctgttaaa aggccggtgc acacgcatcg gcttttttat ttttgacttt
     5761 tctgagtgaa ttggttaatt tcgcacgcat ttttttaacc ataggaacca tactgccgtg
     5821 attgaaattt ccgacatcga aaaaaaactc gaactcgctc aatttaaaat ttcagaattg
     5881 cggaggtttc tttgacgtcg atcaacgtga tcaaaaaata gccaacctcg aagcgcagac
     5941 taccgttgaa gatttttgga atgacaatca gcgtgcgcaa cagatcatgc aacagatagc
     6001 ctctgagcgc gcatgggtag atcaatggaa aaagctggat gcgcaatgcg aagatctgaa
     6061 tatgctttta gagatcgctg aagaggaaaa tgacgaagct acaatcaagt ccattgatac
     6121 cgaactgact gagcttgaaa aggcgcttgc acgcctggag ttacgcgctt atttcacgaa
     6181 tccggaagat aacaaaagca ctgttcttac gattcaccct ggtgccggcg gaacagaatc
     6241 ccaggattgg gcagagatgc ttatgcgtat gtaccaacgc tgggccgccg atcgcggcat
     6301 gaagtgccag attctcgata ttcttgacgg cgatggtgca ggtattaaga gcgtgacgat
     6361 ggaaatttcc ggtgaatttg catttggtct gcttaaatgt gagagtggcg tacaccgttt
     6421 ggtgcggata tccccttttg actccaatgc tcgccgtcac acgtcatttg cgtccgtata
     6481 cgcctatccg gaaatggaag aagtcgaagc caagattgaa atcaatccag cggatatcaa
     6541 aatggatacg tatcgctccg gaggtaaagg tggtcagaat gtaaacaaag tagaaacagc
     6601 cgtgcggttg acacatattc cttccggcat cgtcgtggca tgtcaggtag aacgttcaca
     6661 gcataaaaac cgcgatctcg ccatgaagct tctgatctcc aagctttatc aaatgcgtaa
     6721 aaaagaagaa caagataaat tagacaaact tgagagcgcc aaatccgata tcgcatgggg
     6781 caatcaaatc cgttcttatg ttttccatcc gtataatctc gtaaaagatc accgcaccga
     6841 taccgagaca agcaacgtgc aggctgtcat ggatggtgat attgatatgt ttatcgaagc
     6901 ctgcctcaaa caaaaagtcg ttcaataata agtcatgaaa agttacctcg aacaacagct
     6961 tcactctgct ctaaaagcac tagccatagc gtccgatagt gaaataattt ttactaaaac
     7021 aaaggacgaa cgttttggta atgtggcgac taatttggca atgaatctcg ccaaaactca
     7081 aaaaatgaat cctcgcgaat tggcacaaaa aattgttgac catctccaac ttgattcatc
     7141 cttggtcacc aaagcggaag tcgccggtgg cggatttatc aatttctttt tcagtcccaa
     7201 ttaccttttt actcagctta aaactatact gctgcaaggt gaagaatatg gtcgtcatcg
     7261 ttttggggcg aatcaacggg tggatgtcga atttgtcagt gcaaatccta caggtccttt
     7321 atctataggt cacgggcgtc aagcggtgct cggcgatgtt ctttgccgat tattatccaa
     7381 tgccggatat caagtaacac gcgaatatta ttttaataat gccggcgttc agatgaaaaa
     7441 attaggtgaa tccgtacgcc tacggtattt ggaattgttg ggtgagacca tcacttttcc
     7501 tgaaaaacat tacgagggcg aatatatacg cgacatcgct gccgacataa aatcacaaca
     7561 cggcgaatcc tggaaatctt atgacttcga acctttcaag tcctttgccg aggatatctt
     7621 atttgccaat atccgaaaag tcttaggtcg tatcggacta aaatttgacg tttattacaa
     7681 tgaagactcc ttgtacaaag aaggtaaaat tgaagctttg ctcaaatcgt ttgcggctaa
     7741 aaacctaacc tatgtcaaag aaaacgctac ctggctaaaa acatccgaat tttcttatga
     7801 tcacgggcct aaacccgaaa atgataaagt catcgtaaaa tcaaccggag agccaaccta
     7861 tcgtttgccg gatatggcct accatgttac caaatttgaa cgcgggtttg atcatatcat
     7921 tgacattttt ggcgcggatc atatcgccga atatcccgat gtattagccg gactcaaggc
     7981 cttaggttat gacacctcgc atgtgcgcgt tctcattcat cagtttgtga cattgatgaa
     8041 agagggggaa attctcaaaa tgtccaaacg taaagccaat ttcgtaacta ttgatgaatt
     8101 gattgatttg cttgggccgg atgtgtttcg ttattttctc atcatgcgca gccacaattc
     8161 gcatcttaat tttgatctcg aattggctca aaagcagtct atggaaaatc ccgcgtacta
     8221 tatacaaaat gcccacgcgc gtatttgttc gattgagaaa aaagccggtg aacgcgatat
     8281 tttgttctct gtttccaatg aaattaaatt ggatttattg aaagcagacg aggaaaccac
     8341 catcattgaa aaacttctac aatttccgga actcactgcg cggcttgcta aaaatctcga
     8401 accgcatccg ctcactacgt atcttgagga attggccggc gtatatcatc attatcagac
     8461 agcaggtaag aaaaatccag atctgcgtgt tatcacggag gataaagctt tgtctgaagc
     8521 acgacttgca ttgtgtatcg ctgttcgcat cgttttacgt cgtggtcttg atattttagg
     8581 tatccatgct ccggaatata tggcacgcga tcaggccgat gaaactgttt gaaataaatt
     8641 gactttccgt gaggcgttgt atacattgcg ccgcatttaa ttgaaaattt tcaggagaaa
     8701 aatgtggata tattggtcgt cggttcggtc gctttagatt cggtagaaac accttttggc
     8761 aaagtcgaag acgccgtcgg aggttctgct acttttttca gtgcagcggc cagctatttt
     8821 gctccggtca atcttgtggc ggtcgtaggg aacgactacc ctatgcatgc catcgaatat
     8881 cttaaatcac gccgcgttga ttttaaaggg ctgcaaatcg aacaaggcga gacattccga
     8941 tgggcaggtc gttataatta tgatctgaat caacgcgata cgatttacac ccacctgaat
     9001 gtatttcaaa acttcaaacc acatattccc gatgcgtata aatcatcaca atatgtgttt
     9061 ttaggcaata tcgctcctga gctacagttt gatgtattgg atcaagttca atcgcctaaa
     9121 ttggtcgctt tagatacaat gaatttttgg attgagggta gtcaaaaagc gttacgcaaa
     9181 gttctggaac gcattgacat ctttattctc aacgactccg aagcaagaca actggccaat
     9241 caacccaatt tgatcaaagc cgccaaagaa atttttaaaa tgggtcccaa agcgattgtc
     9301 attaaaaaag gtgaatatgg cgcggcgctc ataacacctg atgctcaatt taatgtgccg
     9361 gcttatcctt tggaagacgt gtatgatccg accggcgccg gtgacacatt cgccgggggt
     9421 ttcatgggtt atattgctaa aacggatgac gcatcatttg aaaatctcaa gcgtgctgtg
     9481 gtctatggca gcgcgatggc ctcattttgc gtacaagact ttagtatcaa cgccttaaaa
     9541 gagctttccc aagataaaat ttttgatcgc gttcttgcat tcaaacaact cagccatttt
     9601 gaagaaaaat agagtggata tttttgtatg aaactgagcg aaattcttaa cgccaaactc
     9661 attcgtatcc cgctactcca atctaacaag caggatatca ttttagaaat gctggatgtt
     9721 ttggttgaat ccggaaaaat cagcgatcgg gaaaaagtat tacaatccgt gcttgaacgc
     9781 gaacaaatga tgagtaccgg tatcggtaac agcgttgcta ttccgcacgg gaaatcctcc
     9841 ggcgtggaag agctgcaagt ggctttggga atcacggctc aggatatcaa ttttgacgcc
     9901 ttagacggca aacctgtacg tatcatattt atgctcgtcg gacccgaaaa ggcttccagt
     9961 ctgcatatca aaatgttatc tcgcatttcg cgattgctca atcaggcacc atttcggaaa
    10021 aaactgaccg atgcccaatc ggccgatgaa ataatgcaaa tcatttcaga tgaagaaaag
    10081 cttttggaag gttaatccgg aagcgtaatg attggttttt cgcggtggca tttatatagc
    10141 aaaatattat tgccgataat ctgaacaatt tgtgactgtg tcccttcgct gatcatttca
    10201 gctacttctt ccttatccaa atcacagccg tcttgcactt tgaccttgat caattctttg
    10261 gtgttaaaac cgttattgac ggataggtac gtattatccg taataccgga cttccctacc
    10321 gaaatggttg ctttaatggc gtttcccaag ccacgtaaaa aacgtttttg ttttcctgtt
    10381 aattccgaca aatgatcctc aatcgttggg taattttctg tgtttatacc aagctgcaca
    10441 aaccagtata atgccggacg ctacaaatgt caacggccaa tacgtacgga tgataaatac
    10501 ccaatcgtat ggaaacatat aaaaattacg tgaaatatga attcctccaa aaccgatcag
    10561 agaaagtcct gcaaccaacg aaaaccagcg tcgcatattg aagaaaaaat tgatgacaaa
    10621 agcgataccg cttatcagaa aatacactgg ccatagcgta cgcatggcga gtccttcctg
    10681 cgtgtcaagg tacaagtgga ataccccgat caacaataac gtcagaccaa aaaacacgta
    10741 atattttttg gttacgatcg tcttgattat gctcacgccg ccaatgataa tcaatccgta
    10801 acgctccatc caaccccagc tcaatacact catattccat agtatccaaa acgtaccgat
    10861 cacaataagc cataaacctg taaacaaata cttcttattg ctgtcggacg cgctatcggg
    10921 cttcacattt gataccgcgg cgtcggatgc gttgtattcg ctttccgttt gcataacgat
    10981 ctcctataat ttcttttccg atgcggcgcc aaaaactttt tgatctttgt catcaggggt
    11041 cagaagtccc acgagcgcat atgcgatcaa gccgacggga aatgcaataa tcgtgataag
    11101 cacccatgct atacgcacaa tcgaaacatc aagattccaa tgtttagcag cgccggcaca
    11161 aacgccgaat atttttttat tttcagacgc acgacgaaac gtactttcac ttgttttatt
    11221 aaaaaatccg gccgaaccgg caaaccggtt acgcgatgct atcaaaaaaa ttccggcaat
    11281 aataaacacc gtcggtacta aaaatttcca tccaaaatgc cgccagaaaa aatggaaatt
    11341 aaaaagattc atcgtatcaa ggaacattat cgcaccaatc aatatcaata tggcgcccca
    11401 aaaaaaacca tggtttacag cgcttcgttt ttcgatcaat tccatatcgg gatcctgcgt
    11461 caaactgacg ggctcgcggg gtacaagaac catcgccgtg atataagcca ccacactaat
    11521 acaatccaca aacgcaaaaa caacaaacaa aagtctcact aaagctacat tcacattaaa
    11581 atattcggcc agacctgcac acacgccgtc caccatttta tcacgacgcg acttaaaaag
    11641 ttgctttgtt gtttccatgg tatctcctga ttcaaaataa tgtccataga taagaacgtg
    11701 tatcttccaa aaatgttgca ttttttagtt aatttgttta gttccaatgc gaggagattt
    11761 gcattgaata atatactact actgagcatt tgctttattt tgggtatagg attgcgcaaa
    11821 agcggacgtt tccatgaaaa aacggcgacc gtgcttaatg cttttattat tcacatcgcc
    11881 ctacccgcct t
//
LOCUS       DAOQNQ010000071        11778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1938_length_11778_cov_5.500128, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000071 DAOQNQ010000000
VERSION     DAOQNQ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11778
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1938_length_11778_cov_5.500128"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..538)
                     /locus_tag="PK754_06360"
     CDS             complement(62..538)
                     /locus_tag="PK754_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09160.1"
                     /translation="MRIITASIQQSDYYFSRKNGTIGMFHEIKNVSQSRGEPFRRWFE
                     DNYFDLVVWYNKNGSFYGFQLSYDRYRHEHAITWFSERGYFHDKVQNTDGSVVTMTPV
                     LVANGPFDGQQIAERFRQHSAGLEIEIAEFIYDKITHYEQIRPSDTKNKKAGRTPL"
     gene            835..2226
                     /locus_tag="PK754_06365"
     CDS             835..2226
                     /locus_tag="PK754_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09161.1"
                     /translation="MKLVYLMAVGIYASFITNQSVYADVTPMQTAVGFNLSIPGKSPL
                     MPNRSMAKKETPTKSVGIAMARESVSIVLYPTMMAVHADFEMENLKSDDISMTVGFPL
                     QAALMSGFPMVDFRVLINGVPVQARYEMLTQDIEPGHTVRETWWSFEVTFSKKQKTSI
                     TVEYRQPFIGRSQQRLLHQGPSADSSYVIQAGYVFRTGALWDGLIGEAELNVRLVGLE
                     KNEMLVFFDGVNQWPEDGRWIKKNWEPASDWFVEIAVHPRRISAMRESASYDVTGLSS
                     IAKMEDRLIRFSRMISEAGDSEKVDTQLPSLDAWKDIVEVIKSAAMQNSDTTLKSWAN
                     RCLAIWRQHLLRSMSLSRDATQLAYALEWLNPDQTPSEEMNEFNRKIKTKPEPGYALQ
                     HVPADKHIMFYLTYPDYSNTVFTDSELFLSVVHEKIRGERFLFFGSIFGGMCVIFVAL
                     IVRTIKRRKKTTF"
     gene            2250..2867
                     /locus_tag="PK754_06370"
     CDS             2250..2867
                     /locus_tag="PK754_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09162.1"
                     /translation="MESNSLDFMRAIGAASAVFVPLIVFYLQKRDKLQKTKYFIDLVK
                     SQDELIKIRDQHASQNGNDIVMMKINSLLSEIEDEINHTVDRERIVSFLLFVFLEVIV
                     LSALIIQWSDYLNKIISGASYESGLFFLEGIFQTTTARLILASMIVSLSVFLTIKSVK
                     KWVDRINRPKLRTTMLVLGFNGVLIVVAIVTSLILAILDPIIPLW"
     gene            2880..3341
                     /locus_tag="PK754_06375"
     CDS             2880..3341
                     /locus_tag="PK754_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNB09163.1"
                     /translation="MRVKLYLLTFYFLISCSAPTSEKTDSEAVKKAIQSANREYMQAY
                     EKSEATRIAALFAEEGVVLQANIEPVRGKSLIERQFVNLFDQSRFTDVRFKTTSIEVG
                     QDMASEYGTYSFSVQPLGLTPATVSGSYLVVWKKQNDGSWKILAEMRQPNI"
     gene            complement(3357..4091)
                     /locus_tag="PK754_06380"
     CDS             complement(3357..4091)
                     /locus_tag="PK754_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09164.1"
                     /translation="MPKLLFIVFTFLMIPASAVEEADRAFYAMDYPLAIRMYETHLNQ
                     KNDSEAYWKLARAYLCSGDISAGVEREVFYRKALTAVESSIQLDRNNSQAYAWFAACL
                     GSVVIFEGSESKIKACYRIKAALDTALMLDPTNDVAWSVLGSYYKALGGINWFERQLA
                     NLFLGGLPEGGYEDAEKALLTAVSLAPDVLRHRFELGVLYYDWDKKDKTQECFEYAMT
                     LKPAMASDNRRISKMKEFMEKHNLPR"
     gene            complement(4159..6408)
                     /gene="pbpC"
                     /locus_tag="PK754_06385"
     CDS             complement(4159..6408)
                     /gene="pbpC"
                     /locus_tag="PK754_06385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HNB09165.1"
                     /translation="MKKNPDLIFIVLAVIGTFFYPISGDDLHFRDEASLRIYDRNGLL
                     LREVLSREGGRGQWVSPDQIDTRIIKAAIAAEDKRFFSHRGMDVLALGRAIGQNIMAM
                     SVVSGGSTISQQVIRNIYHFPRNPFYKLVELWYAIRLEYTLSKNEILAQYLNRIPFGN
                     QAFGVEAASRLYLGKSSQTVTWSEAAFLMALPKSPTRYNPYRNDTAAKNRRIAVLQRL
                     LVMQAIPNDDYERAVSEPILLHTQPHNFFAPHFTDYLLQHHAHLSTAWHTTLDLPLQQ
                     SAERILREHITYLQPENVTNAAAIILDNRTGALRVMAGSYDYFDAQHDGQFNAALARR
                     QPGSALKPFTYAIAFERNYTAASILADVQASFSTANGTFTPMNYDRRYHGPVRARIAL
                     ACSYNIPAVRVLQDVGVPVLLSKLHECGIQSLDQSPAYYGHGLTLGNGEVTLLELTRA
                     FSVLANQGALIQTQVFSDSVSRVERERIFTPESIFLVSDILSDPIARIPAFGYDSPLS
                     FPYPVAAKTGTSSDFRDNWTIGYTRDYTVGVWVGNFDNTPMDRISGITGAGKIFHALM
                     DALYPSTAPSGFVAPHTLHKIPICALSGQRAHPGCGAVIDEYFMKISSVQTTCTWHDQ
                     RGIIRYETIPQEYRAWFSSAGNHASVHASAPDNISNTVQIIHPSNNAVYKIDPNLRLS
                     HQSITFTVTASNQTKRIRWYINGTLYRETHDAFELVWPIKPGKYTLRVQAEQAIQTSE
                     STVHFEVLP"
     gene            6539..7258
                     /locus_tag="PK754_06390"
     CDS             6539..7258
                     /locus_tag="PK754_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006196901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNB09166.1"
                     /translation="MYNKGYLAAGIGVFFLYFALSPVHAQTPTKPSMWYLTDLEKEVV
                     RELNRVRRDPQGYVTHLKALKKLYDGNMVYYPGEATLMTNEGVDAVDECIRFLENAQP
                     IDTLGPSEGMSKAARDHMKEQSRSGKTGHTGDDGSSPFDRIERYGQWERTAGENIDYG
                     SNDARRIVISLLVDDGVPSRGHRENIFNPEFRKVGVACGTHKQYRYMCVMTLAGGFSA
                     KKNTIHTRGENPADGDATGDK"
     gene            7270..7944
                     /locus_tag="PK754_06395"
     CDS             7270..7944
                     /locus_tag="PK754_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09167.1"
                     /translation="MNYLSHFAINQKKQVSPQFHLGAALPDLMSAINRTLRFQENAVR
                     AYIPHAHCPDVWHGVLNHFEADAAFHGSSFFVSCYETIRELLKENIAPQKNIRPFFLA
                     HIVVEILLDHVLSSNDTSLASRYYRNLERIVVPTIQTDVDRYFGNSTSEFTEIFDRFM
                     RFKFLYEYDQIKKMHEPINRMLRRTRQDGFAEHEIADLIAILETCKHIIVKALPEFEA
                     QFMPQD"
     gene            7881..8864
                     /locus_tag="PK754_06400"
     CDS             7881..8864
                     /locus_tag="PK754_06400"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB09168.1"
                     /translation="MQTYHSKSVAGIRGAIYAAGLICFLSAVISCDNSDTDHRIWYVA
                     DKRIYVYAIGESRDYYWVSAQEKILRISKNDGDTLSITYGTQGFPANAVGCFFPADSG
                     RIWMGTGGGGLLLVDTIVVRRYTTSNSQIPSNGVQAIAQSSDGVFWLATNGGLVKFDN
                     HNFTTFNTSNSGLPANALTSVGVGDDKSVWAGTWGFNLARFKENQWTIFTKNNSSLQT
                     AYIFTLLHTGKTLWVGEGGGINRYTASGWKNYNSKDTDLPQNDVTTIVVDKYGLWVGT
                     YAGGVGFLKSETWTVYNTTNSELPDDQVTAMFADRRGRLWVGFYRGLAVFQ"
     gene            9002..10069
                     /gene="rsgA"
                     /locus_tag="PK754_06405"
     CDS             9002..10069
                     /gene="rsgA"
                     /locus_tag="PK754_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNB09169.1"
                     /translation="MTLDHLGWQPFFDTAFKPYAGEFQVGRIAVQQTRVYELWTEAGV
                     ISAEMSGNLRKSAEIDSTLRPVVGDWVVIQLRLAERFAVIHHVLPRRTKFSRKVTEEV
                     TREQVIAANIDIAFLVNGLDQDFNLRRLERYLLTAQHSGAEPVIVLNKVDLCAESDAR
                     LSEVRAIAPSVPIITMSAMHKQGIELMNFYLGQGKTGCLLGSSGVGKSTIINALLDDD
                     VQSTRTTRKDMDRGRHTTTHRELFVLPGGGLLMDTPGMRELQLWTDESSLSGTFEDIE
                     TLAAACRFRDCRHESEPDCAVQAALADGSLDAARYKSYLKLRREIRYLELRQDNSAAR
                     LEKMRWKKIHAQHKKNNRSKN"
     gene            10187..10696
                     /locus_tag="PK754_06410"
     CDS             10187..10696
                     /locus_tag="PK754_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HNB09170.1"
                     /translation="MKQKLESAFESIIFASRWIQAPLYAGLIVGGILYAYKFLNELIH
                     LCLTINEISESVLMLGVLTLVDITMVANLLVMVIIGGYSTFVSRMNIDEHEDKPEWLQ
                     KVDAGTLKVKLAGSLVGVSGIHLLQVFINIKNHNSEQVMWQTIIHVVFLFSAIMLAYS
                     EKLLHEKHG"
     gene            complement(10711..>11778)
                     /locus_tag="PK754_06415"
     CDS             complement(10711..>11778)
                     /locus_tag="PK754_06415"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNB09171.1"
                     /translation="YENGVKLLIGSDAGFHFAIHGFSYLEEVQNFAELGIPHYDILKS
                     GTSSAAEFLGWDDKIGTVSIGKEADLLLVPENPLEKITTLAHHNGVMSRGVWYSRSKL
                     NESLRSLKNELRDMPGSDRFSEFSAPDKNYHLLAHYAISYKDIRCGDEKIFSSINSKN
                     QMRLLSQNSIDPPCQRNTTTTWLIDNENILFTDVHRTSIEGSTQVTMTHQFPSSTRVH
                     GNHPVYGEFDYVDSLNTYRITGGPNTSINLDMDIVANYHMLFMKLKPMEVGEADSLTS
                     KKIELNAEEWGKRCITGDTWYKIKRRPDNAFGERNYEIIQPGFNGSSEFSFQSIITLG
                     NDGIIKDIRFNNHVTAKRIVP"
BASE COUNT         3201 a         2472 c         2815 g         3290 t
ORIGIN      
        1 acggaaattt tcggagaaca agaaaaagaa ataaagatta aatcgccggt acgatcgttt
       61 atcaaagcgg ggttcgcccc gcttttttat tttttgtatc ggatggtctt atttgttcat
      121 agtgtgttat tttatcgtaa ataaattcag caatttcaat ttctaatcct gcactgtgct
      181 gacgaaagcg ttcggcgatt tgttgcccat cgaaagggcc gttggctaca agtacgggcg
      241 tcatagtgac caccgaaccg tcggtgtttt gcaccttatc gtgaaaataa cccctttcgg
      301 aaaaccatgt tatggcgtgt tcgtgacggt atcgatcata ggacaattga aagccataaa
      361 aacttccatt cttgttatac cataccacaa gatcaaaata attatcctca aaccatcttc
      421 gaaaaggctc tccacggctt tgggatacgt tctttatttc gtgaaacatg ccgatagtcc
      481 cgttttttct actaaaatag taatcagatt gttggatgga tgctgtgatg attcgcacag
      541 tgacaaataa ttttttacag tgcgggaaat ttatgcaaaa tgttacatat aattatacct
      601 tattgacaaa tgcagtgaaa tgaagggctt tttatcaatc gctaacggca attattcaaa
      661 gtgtataaaa gttcagtgta tctgacttcc tcaagataac ttaaaacaaa accgtttgcc
      721 ggacttgaaa acacactttc aatatcaaga ataagaaaca ttcgacgtat tgcgttatac
      781 tttggattcc actacattgg agccgttcac aaaaattttt tgctcgaaag atttatgaag
      841 ctcgtttact tgatggctgt cggaatttat gcgtcgttta ttacgaacca gtccgtatat
      901 gcggatgtaa caccgatgca aaccgcggtc ggttttaatt tatccattcc gggcaaatcg
      961 cctttgatgc cgaaccggag tatggcgaaa aaagaaacgc cgacaaagtc tgtgggcatc
     1021 gctatggctc gtgaatcggt ttcgatcgta ttatatccta cgatgatggc agtgcatgcc
     1081 gattttgaaa tggaaaatct aaaatccgac gatatatcca tgaccgtggg atttccgctt
     1141 caggcggctt taatgtccgg ttttccgatg gttgattttc gcgtgctaat taatggcgta
     1201 cctgtccaag cacgttatga aatgctaact caagacatcg aacccggtca caccgttcgc
     1261 gaaacatggt ggtcatttga agtcacgttt tccaaaaaac aaaagacttc cataactgtc
     1321 gaataccggc aaccgtttat cgggcgtagc caacaacgtt tattacatca aggtccttcg
     1381 gccgattcgt cgtatgttat acaagccggt tatgtttttc gtaccggcgc tctgtgggat
     1441 ggactcatcg gcgaagccga actcaatgta cgtttggtcg gtttggaaaa aaatgagatg
     1501 cttgtttttt ttgacggcgt aaaccaatgg cctgaagacg gacgctggat caaaaaaaac
     1561 tgggaaccgg catcggattg gtttgtagaa atcgctgttc atccgcgtcg tatttcagcc
     1621 atgcgagaat ccgcttcgta tgatgtgacc gggttgtcgt ccatagccaa aatggaagat
     1681 cgtttaatac gattttcacg catgatatca gaggccggtg attccgaaaa ggtagatact
     1741 cagcttcctt cattggatgc atggaaagat atcgtcgagg ttatcaaatc agcggcaatg
     1801 caaaattcgg ataccacatt aaaatcgtgg gcgaatcgct gtttagccat ttggcgtcag
     1861 catttgctgc gtagtatgag tctaagtcgc gatgcgactc aactggccta cgcattggaa
     1921 tggctcaatc cggatcaaac gccgtcggaa gaaatgaatg aattcaatcg gaaaataaaa
     1981 accaaacccg agcccggata tgcattgcaa cacgttcctg cggataaaca tatcatgttc
     2041 tatctgacat atccggatta ttccaatacg gtttttaccg attcggaatt gtttttaagt
     2101 gtcgtgcatg aaaaaataag aggggaacgt tttttgtttt tcggatctat tttcggtggg
     2161 atgtgtgtta tttttgtcgc ccttattgtg cgaaccataa agcgcaggaa aaaaactacg
     2221 ttctagtttt tacttaaacg gaaaaatgta tggaaagtaa ttcattagac ttcatgcgcg
     2281 cgatcggtgc ggcttcggcg gtgtttgttc cgctgattgt tttttacctt caaaagaggg
     2341 ataaactcca aaagacgaag tattttattg atttggtcaa atcgcaggac gaactcatca
     2401 aaattagaga ccaacacgcg tcccaaaacg gcaatgacat cgtgatgatg aagatcaatt
     2461 cgctcctatc ggaaatcgaa gatgaaatca atcataccgt ggaccgggag cgtatcgtaa
     2521 gttttctcct tttcgttttt ttggaggtga ttgtgcttag cgcattgatc atccaatgga
     2581 gcgattatct gaacaaaatc atttccggcg cttcgtatga aagcggcctg tttttccttg
     2641 aagggatatt tcagacgaca acggcccgtt tgattttggc ttcgatgatt gtcagtcttt
     2701 ccgtttttct cacgatcaaa agtgtaaaaa aatgggttga ccgtatcaat cgccccaaac
     2761 tcagaacgac gatgctcgtg ctcggcttta atggcgtact cattgtcgtg gccatcgtta
     2821 caagtctgat cctggctata cttgatccga tcattccatt gtggtaaaag gagcaaacca
     2881 tgcgagttaa gttgtacctg ttaacgtttt atttcttgat ttcatgcagc gcaccgactt
     2941 ctgaaaagac ggattccgaa gcggttaaaa aagctattca gtccgccaat cgcgaataca
     3001 tgcaggccta cgaaaaaagc gaagcaaccc gcatagctgc tctatttgcc gaagaaggtg
     3061 tcgtattgca ggcgaatatc gaaccggtgc gcggtaaatc actgatcgag cggcagtttg
     3121 tcaatttatt tgaccaatca cgttttacgg atgtacgatt taaaactacg tccatcgaag
     3181 taggacagga tatggcttca gaatacggta cgtacagttt ttccgtgcag cctttggggc
     3241 taactccggc tacagtctcg ggctcttatc ttgtggtttg gaaaaaacag aacgacggct
     3301 cgtggaaaat tttagcggaa atgcgtcaac cgaatatttg aattggcgta ttccgatcaa
     3361 cgcggcaggt tgtgcttttc cataaattct ttcattttgg aaatgcggcg attatcgctg
     3421 gccatggccg gtttgagcgt catggcgtat tcaaagcatt cctgcgtttt atctttttta
     3481 tcccaatcgt aataaagaac accgagttca aatcggtggc gcagtacatc cggcgctaag
     3541 gaaacggccg tgagtaacgc tttttccgcg tcttcgtatc cgccttccgg taatccgccg
     3601 agaaacaaat tagccaattg acgttcaaac caattgattc cgcctaacgc tttataatac
     3661 gaacctaaaa cagaccacgc gacgtcattg gtcggatcaa gcatgagcgc cgtatccaaa
     3721 gccgctttga tccggtagca agctttgatt ttagattcac tgccttcaaa aattaccaca
     3781 ctaccgaggc atgcggcaaa ccacgcatac gcttgcgaat tattgcgatc aagttgtata
     3841 gaggattcca cagcggtcag cgctttacga taaaacacct cacgttccac tccggcactg
     3901 atatccccgg aacacagata cgcgcgggcc aacttccaat acgcttcgct gtcatttttt
     3961 tgattcagat gcgtttcata catacggatg gccagcgggt aatccatcgc ataaaatgcc
     4021 cgatccgctt cctctacagc agaagccggt atcattaaga atgtgaacac gataaaaagt
     4081 aattttggca tagcgtagtt tactacgaag ttattttgaa tttataaggc caatgttatc
     4141 gaatcgtaaa tacttgtatt acgggagcac ttcaaagtgt acggtagatt cgctggtttg
     4201 aatggcctgt tcggcttgta ctctcaatgt atatttcccc ggtttaatag gccatactaa
     4261 ttcaaatgca tcatgcgttt cacgatacaa agtcccgttg atataccagc gaatgcgctt
     4321 tgtttgattg gacgcggtga ctgtgaaagt tatactttgg tgcgacaaac gcaaattagg
     4381 atctattttg taaacggcgt tattcgatgg atgtatgatt tgtacggtat tcgaaatatt
     4441 gtcgggtgcc gatgcgtgca ccgaggcgtg atttccggcg ctgctaaacc atgcgcggta
     4501 ttcttgcggt atcgtttcat aacggattat gccgcgttga tcgtgccatg tgcaggtggt
     4561 ctgcaccgat gaaattttca taaaatattc atcaatcacc gcgccgcagc cgggatgcgc
     4621 acgttgtccg gatagggcac agatcgggat tttatggagt gtatgcggtg caacaaaacc
     4681 ggacggcgct gtcgagggat ataaagcatc catgagagca tgaaaaattt ttcccgcacc
     4741 ggtaataccg gaaatgcggt ccatcggtgt attgtcaaaa ttaccaaccc acacaccgac
     4801 ggtataatca cgagtatacc caattgtcca attatctctg aaatcgctgg atgtacctgt
     4861 tttggcggcc accggatatg ggaatgaaag cggcgaatca taaccgaagg ccggaatgcg
     4921 ggcgatcgga tcggataaaa tatccgatac gagaaaaatg ctttccggcg taaaaatacg
     4981 ctcgcgttcg acgcgggaca ccgagtcaga aaaaacttgt gtttggataa gcgcgccttg
     5041 attggccaaa acagaaaatg cgcgtgttaa ttccaataac gtgacttcac cattacccaa
     5101 tgtcaatccg tgaccgtaat atgcaggcga ttgatcgagg ctttgtattc cgcattcatg
     5161 cagctttgat aataaaacgg gtactcccac atcctgcaaa acacgtacgg caggaatatt
     5221 gtatgagcaa gccaaagcaa tacgcgcccg caccggccca tggtaacgtc ggtcgtagtt
     5281 catgggtgta aatgtgccgt tcgccgttga aaacgatgct tgtacgtcgg ctaaaatgct
     5341 ggccgcggtg tagttgcgct caaaagctat tgcgtatgta aaaggcttca aagccgagcc
     5401 gggttgccgc cgtgcgagcg ccgcattaaa ttgaccgtcg tgttgcgcat caaaataatc
     5461 atacgaaccg gccatgacgc gtagagcgcc cgtacgatta tctaaaatga tggccgccgc
     5521 attggtgaca ttttccggtt gcagataggt gatatgttcg cgaagtatgc gttcagcgga
     5581 ttgttgcaac ggtaaatcca gcgtggtgtg ccacgcggtg ctaagatggg catggtgctg
     5641 taacagataa tccgtaaagt gcggtgcaaa aaaattatga ggttgcgtat gaagtagtat
     5701 tggttcgctt acggcccgtt cgtagtcatc gttgggaatg gcctgcataa caagaaggcg
     5761 ctgtaaaacg gctatgcgcc tgtttttagc ggccgtatca ttgcggtagg gattatagcg
     5821 cgtgggcgat ttgggcaaag ccatgagaaa ggccgcctct gaccatgtta ccgtttgtga
     5881 cgatttgccg agatagagcc gggatgcggc ttccacacca aaagcttgat taccgaacgg
     5941 gatacgattg agatattgcg cgagaatttc attttttgat aatgtgtatt ccaggcgaat
     6001 cgcataccac aattcgacca gtttgtaaaa cgggttgcgc ggaaaatgat agatatttcg
     6061 gatgacttgc tgggaaatgg ttgacccgcc gctgacgacc gacatcgcca tgatattttg
     6121 tccgatagca cggcccaggg ccagtacatc catgccgcga tgcgaaaaaa aacgtttatc
     6181 ttccgccgcg atagccgctt tgattatccg cgtatctatc tgatccggtg aaacccattg
     6241 accgcgtccc ccttcacggg ataatacttc acgaagtaaa agtccgttgc gatcatagat
     6301 acgcaaggag gcttcatccc taaaatgtaa atcatcaccg gaaatcggat aaaaaaatgt
     6361 tccgataaca gccaatacga taaaaatcag atccggattt tttttcaaag ttttgagcat
     6421 gtcagagtgt agagagttta atatttaatt tcaaaaacaa atgtaaacat atagaggtaa
     6481 cgtttgctat tcaccgaggg gcttgcgata ttgcaaaaaa ataaaaggag cttccgacat
     6541 gtataacaaa ggttatctcg ctgcagggat aggagttttt tttctttatt tcgcattatc
     6601 tcccgtgcat gcacaaacac cgactaaacc ttcgatgtgg tatctcacgg atctcgaaaa
     6661 ggaagtggtg cgcgagctta accgtgttcg tcgtgatccc caaggatatg tcactcatct
     6721 gaaagcgctc aagaaattat atgacgggaa tatggtgtat tatcccggcg aagcgacgct
     6781 catgaccaat gaaggcgttg acgccgtaga tgaatgcatc cgatttcttg aaaacgcgca
     6841 gcccattgat acgttgggac cgtcggaggg tatgtccaaa gctgctcgcg atcatatgaa
     6901 agaacaaagc cgttccggta aaaccgggca taccggcgat gacgggagtt cgccctttga
     6961 tcgcatcgaa cgctacggac aatgggaacg tacggccggt gaaaatattg actatggttc
     7021 caatgacgcc cggcgtattg tcatatcgct gctcgtagat gacggcgtac cgagtcgcgg
     7081 ccacagagaa aatattttta atcctgaatt tcgtaaagtc ggcgtagcct gtggtacgca
     7141 taaacaatat cgttatatgt gcgtcatgac tttagccgga ggatttagcg ctaaaaaaaa
     7201 tacaatccat acccgcggcg aaaatccggc ggacggcgat gcgacgggcg ataaataaag
     7261 cgttgatgaa tgaattatct ttcgcatttt gcgataaatc aaaaaaaaca agtatcccct
     7321 caatttcacc ttggcgcggc gctgccggac ctgatgagtg cgatcaaccg tactttgcgt
     7381 tttcaggaaa acgcggttcg tgcttatatt cctcatgcgc attgcccgga cgtttggcat
     7441 ggcgtgctca atcactttga ggccgatgcg gcttttcacg gttctagttt ttttgtttcg
     7501 tgttacgaaa ccatccgcga gttgctcaaa gagaatattg cacctcaaaa aaacatcaga
     7561 ccgttttttc tggcgcatat tgtcgtggaa atattactgg atcatgtttt gtcgtcaaac
     7621 gatacctcgc ttgcatcgcg ctattatcgg aatctggaac gtatcgttgt accgacaata
     7681 caaacggatg tggatcgtta ttttggtaat tcgacatctg aatttacaga aatttttgat
     7741 cggtttatgc gttttaagtt cttatacgaa tacgatcaaa tcaaaaaaat gcacgagccg
     7801 atcaaccgca tgcttcggcg cacacgacaa gacggttttg cagaacatga aattgcggat
     7861 ttgatagcga tcttagaaac atgcaaacat atcatagtaa aagcgttgcc ggaattcgag
     7921 gcgcaattta tgccgcagga ttgatttgtt ttttatcagc tgtcataagt tgtgataatt
     7981 cggataccga tcaccgtatc tggtacgtgg cggataagcg gatatatgtg tatgcgatcg
     8041 gagaatcccg tgattattat tgggtatctg cgcaggaaaa aatactccgc attagtaaaa
     8101 acgatggtga caccttgtct ataacgtatg gtacacaggg tttcccggct aacgcggtcg
     8161 gatgtttttt cccggcggat agcggtcgta tttggatggg aacaggcggc ggcggtttgc
     8221 tattggtcga tacgatcgtt gtgcgccgtt atacgacatc caattcgcaa attccatcga
     8281 atggcgttca ggctatcgct caatcatcgg atggtgtttt ttggctggct accaatggcg
     8341 ggttggtaaa atttgataac cataatttta caacgttcaa tacaagtaac tccggattac
     8401 ctgcaaatgc tctgacatcc gtaggtgtgg gcgatgataa gagcgtttgg gccggtacat
     8461 ggggtttcaa tcttgcgcgt tttaaagaaa atcaatggac gatatttacg aaaaataatt
     8521 cgtcgcttca gacggcttat atttttacac tgcttcatac cggtaaaacg ttatgggtcg
     8581 gtgaaggcgg aggcatcaat cgttatacag cctcgggatg gaaaaattat aacagcaaag
     8641 atactgatct tccgcaaaat gatgtaacga ccattgttgt ggataagtat ggtttgtggg
     8701 taggtacgta tgccggtggc gttggttttt taaaaagtga aacgtggacg gtatataata
     8761 ctacgaatag cgaacttcct gatgatcaag taacggctat gtttgccgat cgtcgaggcc
     8821 gtttgtgggt tggtttttat cgcgggttgg ctgtttttca ataaaattgt taattattta
     8881 tatcactttt tacacaaagg agagtctatg attgccgtca ttgttttcaa gtttcatgaa
     8941 aacttattaa ctacgtaacg gagctttgta ctccgttgct aaaacaatgg agcaaaatca
     9001 aatgacgtta gatcatttag ggtggcaacc cttttttgat acagcattca aaccttacgc
     9061 cggtgaattt caggtagggc gtatcgccgt tcagcagacg cgggtttacg aattgtggac
     9121 ggaagccggt gttatttcgg cagaaatgtc gggcaatctt cgcaaatcgg cggagattga
     9181 ttccacattg cgacctgtcg tcggtgattg ggtcgtgatc cagttacgtt tggccgaacg
     9241 ttttgccgtg atacatcatg ttctcccgcg ccgaaccaag ttttcgcgta aagttactga
     9301 agaggttacg cgcgagcagg tgatcgcggc gaatattgac atagcttttc tggtaaacgg
     9361 tctggatcag gattttaatc tgcgccgact tgaacggtat ttgcttactg cgcagcatag
     9421 cggtgcggaa ccggtgatcg tactcaacaa agtcgatctt tgtgcggaat cggatgctcg
     9481 gttatccgaa gtgcgggcta ttgcaccgtc cgtgcccatc ataacgatga gcgcgatgca
     9541 caaacaaggc atcgagttga tgaattttta ccttggacaa ggtaaaaccg gttgcctact
     9601 cggatcctcc ggcgtcggta aatcaacgat catcaatgcg ttgcttgatg atgatgtcca
     9661 atcaacacgc actacacgca aagatatgga tcgcggaaga cacacgacga cgcatcgtga
     9721 attatttgtt ttaccgggcg gcggtttgct gatggatacg ccgggtatgc gcgaattgca
     9781 attatggacg gatgaaagca gtctttcagg aacgttcgaa gatatcgaaa cgcttgcggc
     9841 ggcatgccgc tttcgggatt gccgacacga gtcagaaccg gattgtgccg tacaagcggc
     9901 attagccgat ggatcgctgg atgcggcgcg atacaaaagc tatctgaaac tgcgaagaga
     9961 gattcggtac ttggaattgc ggcaagataa ctcggccgca cgattggaaa aaatgcggtg
    10021 gaaaaaaatt cacgcccagc ataaaaaaaa caatcgctct aaaaactaaa caaaaaagcg
    10081 atagcttgtc acagcccggt tctgtagtaa tacaacccgg gcttgttttt taatcgaatg
    10141 tcgttaaatt gaccacgcaa aataattaca ggagaagttt tttgctatga aacaaaaatt
    10201 ggaatccgct tttgaaagta tcatttttgc cagccgatgg atacaagcgc cgctttatgc
    10261 cgggttaatt gtcggcggaa ttttatatgc atataaattt ctgaatgagt tgatccacct
    10321 gtgccttaca ataaatgaaa tcagcgaatc cgtacttatg ctcggggttc ttacgttggt
    10381 ggatattacg atggttgcaa acctgcttgt gatggtgatt atcggcggat attcgacgtt
    10441 tgtcagccga atgaatattg atgaacatga agataaaccg gaatggctgc aaaaagtaga
    10501 tgccggtacg ctcaaggtga agctggcggg atcgctggtt ggtgtttcgg gtattcacct
    10561 tcttcaggtt tttattaata taaaaaacca caattccgaa caagttatgt ggcagacgat
    10621 tattcacgtg gttttcctat tttcggcaat catgttggcg tactcagaaa aactgttaca
    10681 cgagaagcac gggtagtcga tatttttact ttacggcaca atgcgtttgg ccgtaacatg
    10741 attattaaat cggatgtctt tgattattcc gtcattaccc agtgtgataa tagattggaa
    10801 tgaaaattcc gaagaaccgt taaatccggg ttgtattatt tcataatttc tttcaccgaa
    10861 tgcgttatcg ggtctacgtt ttattttgta ccacgtatcc ccggtgatgc atctttttcc
    10921 ccattcttcc gcatttagtt cgattttttt agacgtcagt gaatccgctt ctccaacctc
    10981 catgggtttt aacttcataa acaacatgtg gtagttagca acaatatcca tatccagatt
    11041 gatggaggta ttggggccac cggtaatgcg gtacgtgtta agcgagtcca cataatcgaa
    11101 ttccccgtaa acagggtgat tgccatgcac acgggtagag gaggggaatt ggtgcgtcat
    11161 ggttacttgg gttgatcctt cgatggacgt acgatgtacg tcggtaaata agatattttc
    11221 attgtcaatc agccatgttg tggtcgtgtt acgttggcaa gggggatcta tgctgttttg
    11281 actgagaagc ctcatttggt ttttgctgtt tatggagctg aatatttttt cgtccccgca
    11341 tcgtatgtct ttgtaagaaa tagcataatg agcaagaagg tgatagtttt tatccggagc
    11401 agaaaattcg ctaaaccgat ctgaaccggg catatctcgc agttcgttct ttaacgatcg
    11461 taaggattca ttaagttttg aacgcgaata ccagacaccg cgtgacatga caccgttgtg
    11521 atgggccagg gttgtgattt tttcaagtgg gttttcgggt accagcagga gatcggcttc
    11581 tttacctatg gaaacggttc cgattttatc atcccatccc aaaaactccg ctgccgatga
    11641 agtcccggat ttaagtatgt cgtaatgggg aatgcccagc tcggcaaaat tttgaacctc
    11701 ttctaaatat gaaaaaccat gtatggcaaa atgaaatccg gcatcggatc cgatcaaaag
    11761 ctttacgccg ttttcata
//
LOCUS       DAOQNQ010000072        11768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1941_length_11768_cov_5.783403, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000072 DAOQNQ010000000
VERSION     DAOQNQ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11768
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1941_length_11768_cov_5.783403"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..5799)
                     /locus_tag="PK754_06420"
     CDS             complement(163..5799)
                     /locus_tag="PK754_06420"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNB09172.1"
                     /translation="MGSKVKKMIRLLLSISVVFLSSIHLNGCIAPDAMQATPMPETVF
                     GSGDFVLSATPQGTVADIQQFYAVVVIFAKPMVALSALPEGDGDGPLEISPSVNGKFR
                     WMGTNTLSFIPSEKLPLNTRFTVTIPAGTRALDGSAMSSPYVFSFETMRPKLISHNIS
                     EMNGTTLNPKIMLTFNQPIDIHSAKEKIRLIRIQDGANIPVSIEPADSADLLTYYNYG
                     YDQVAGSPVERIVRVKPITSLRMQTSYQLQIASGLKGREGVLGSSERVTHDISTYGPL
                     EFLELIPPEYPNSPFQLKFTNRIDADELKSHLTITPHTEINEIYPSWSSEYPASIYAQ
                     LQPQTDYTITIDPEIKDAFDNALGVTVRQNFRTGDYASSVYFETGEHVLESYLRHDLN
                     VAMINPGNIDIQMAAITQEEVIGLAKEGLYRDNGFNRWAHTISERPDLPRNATQYYPL
                     ALDPALASQPSGFVFVQMKHDTWNDYQRALIQVTDIGITAKFARDKSVIAVSQLKDGR
                     AARAFVQIRDDMGNLLWSGYTNSDGVAETQGWSGFGLQPIDTYNEPRLWVFATRGDQT
                     AYARSDNKIDLWRFNIPYAWYVQSAEDEISSKLFTNQGIYRPGDKVQFKAMLRRLQKG
                     AWSVPTGLNLKARIFNPSNDEVYAQDIVVNPFGGTDFEFTVPENAPLGYYYARLYTSE
                     KNKELSGESFQVQEFRPVESAVTINSVKSEYVWSETLKLNFDAHYLFGAPMANASMNW
                     SITRNASSFQPEGYDGYFFGKMYDYDGYYGRGAYQTVLASSSGTLDQHGLMDADVVLN
                     AGGVSETSELVIEGTVEDKNRQYVSGRTTVRVHQGAFYIGLKPSTTFMTWGEPLSVDV
                     VAVTPDGKKTADKKIELELIRREWHSVREKSYDGSYRWTSSQRDTTEEVFTVRSRGEI
                     VTQKLNPASTGYYILRATSSDPYDNTIISESYVYVTGESYAGWKLNDGDRIDLVANKS
                     QYKVGEKAKILVKSPYERARALVSVEREGIIRHFMTELKGNAASVEIPITAEMAPNAF
                     VSVTLLQGRTAEPVPDQNEDIGKPSFKIGYINLSVDPSANKLAVKIQTPKNKFQPGEW
                     MDVDVEVADKSGHGQIAEVTLYVEDVGVLNLVGYQTPDVFSHFYKQRELQVATTESRR
                     FILDQLVKRDLKEKGGVGGGGGEERSMPGIAMRKDFKSCVYWNPSVITDARGKARVRV
                     QLPDNLTMFRIFAVAHTRDSRFGSDDHSITVSKDLMLRPALPRFARIDDAVEAGAVLH
                     NNTTQAGTATIRAQAEGVEIIGAIEKTVELQPGASQEVRFQYRALKTGPAKFSFTAQM
                     RQFSDGVEITIPVRMPVPTETVALAGSTTDKTQEQVIIPGSIFENTGSFEIKTSSTAL
                     VDLDGSVKYLFEYPYGCLEQRTSRVLPVILFGDVVTAFGLTPFEDKTKSVDAAVKEYL
                     NEIPKFQKYSGGFSYWTGDHIVSPYVSVYAMFALTKAKAIGYDVPKEIMDNGVEYLKK
                     LVRNNPVDRYGVFYWHNTNAFALAVLADHDVYDAASAELLFQRRSELPLFARAMLLQA
                     IVKGGGHKAMTETLRRDLMNAIKMNPTTAHFEEPNLPGSEWSFHSPMRTTAAILQTFI
                     ELDGTNVPWAEKVVKYMLEERKQGRWRTTQENLYVFYALGTYFRNFEDVDPDFKATVR
                     LAGRQLLSQMYQGRTTQSHTSFTALAEFPSGKALLLDITKTGAGRLYYNARMTYAPRS
                     GGNAPARDEGLRVSKEIQDASGHVVTDGRYKAGKIYKITLTVSTAQARQYVVLDDPLP
                     AGFEAINTDLATSASSYGSGTENRWWEYGGFTRSEMRDDRVVAFADYFENGTQKFVYY
                     VRATTYGTFALPATKAEAMYHPEIFGTTAATQISVE"
     gene            complement(5810..6088)
                     /locus_tag="PK754_06425"
     CDS             complement(5810..6088)
                     /locus_tag="PK754_06425"
                     /inference="COORDINATES: protein motif:HMM:NF037968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HNB09173.1"
                     /translation="MDTMIVGLVSGILTTFGFLPQVIKSLRTKKVEDVAIAQPVVLLI
                     GMSGWLLYGVMRDDLVIIMANIVSIALNLWLIVLKIKYRSNVTANNGK"
     gene            6174..6659
                     /locus_tag="PK754_06430"
     CDS             6174..6659
                     /locus_tag="PK754_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09174.1"
                     /translation="MKDISGAINNLLNKFKIWVLLGGVVIITIAFTAPQFLFILIFPL
                     FFGLFFYIFKSFVQPMMRKMDAQFGIKLPPEFDESQETTRDISRWDAILSCAQSKGGR
                     LTAADVSIYFNVDLQTAEVRLDQCVESGHADLYVSQSGVKVYQFSHIISDDEKKRAEP
                     V"
     gene            complement(6710..8491)
                     /gene="aspS"
                     /locus_tag="PK754_06435"
     CDS             complement(6710..8491)
                     /gene="aspS"
                     /locus_tag="PK754_06435"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNB09175.1"
                     /translation="MSAIMNTAHRSTTCGELRAANAGQKVKLIGWIHRRRDLGQVIFI
                     DLRDRYGLTQIVFNPDKFPETHEKAKELRSEFVIKVLGVVTPRESVNKNIPTGEIEVI
                     TESLTIMSRAKTPPFEINDENLKVDEDLRLEYRYLDLRRSYMQETLVLRHRLAQIVRN
                     YFTENQFVEVETPVLMKSTPEGARDYLVPSRVHAGKFYALPQSPQIYKQLLMVSGMDR
                     YFQIVKCFRDEDLRADRQPEFTQVDVEMSFVEREDILGMIERLMFKIFKELKDIDLTM
                     PLRRMPYFEAMRMYGSDKPDLRFGMPITYIEECVRGSEFKVFNDVLDQKDGAIALLCV
                     ESQASSFSRKKIDELTEFVKKHGLLGLATVKVEATGITSSIGKFFTEDKLRRMAEVAG
                     AKEGDLLLIAAGKEQIVQSALGALRLKLAEDLKLVRDDAYAMFWVVDFPLFEYDEEEK
                     RWNAMHHPFTSPFDEDLSKMDSEPGAVRAKAYDLVLNGSEVGGGSIRIHSRDVQNKMF
                     DALGLTQEDRDTKFGFLLRAFEYGVPPHGGIALGFDRLTAILAARKSIRDVIAFPKTN
                     SAVSLMDQSPSPVDARQLKELHIRTAE"
     gene            complement(8586..9653)
                     /locus_tag="PK754_06440"
     CDS             complement(8586..9653)
                     /locus_tag="PK754_06440"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HNB09176.1"
                     /translation="MNSVKRNTKAAAAPPGGARAAGAARDIRIVAFPKEFQKSYWSAF
                     DKRMLGIFFASLVIVYAPLIYMAMQPPRPKPTVLDNKLLKAIAKINKVDAALLEELDK
                     PKEEEKTEETATSSVPSAQINRQQAPTNAQGRATRAAAAKAAAAARAGAAAKAAAGRG
                     VLAVAGAVGAGGGGRYAAVDFSGGSGNLDEVLGQVGGIGEATGGGGDRTVLGAGGVAG
                     GTGSISDLTAELGGAGALSVAAGSAGSGLIGVGKASISGTGGGQGASAAEIQGVIDQN
                     ATSVNACYQKELKKSPDLKGKLSVSIKINQAGRVSGVSVTQNTVGDAVANCVTNKIRG
                     WTFPRGKKGVVTVNQTFVFTK"
     gene            complement(9698..10204)
                     /locus_tag="PK754_06445"
     CDS             complement(9698..10204)
                     /locus_tag="PK754_06445"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNB09177.1"
                     /translation="MAFKPSAAKKNKRSEEGTLNMNSMMDILTIMLLFLLMNFSTSGA
                     LATKSDGMQPPKIVTKNKPKKMLLINVSQAHIFYNKDAIAETETVLAQKNSFLIPQLA
                     EKLEEDANKALDLEARFGIEFKREVIVVCDDRTPFNVLMKVVVTCGRNQFSNLRLLGA
                     LANKNDVI"
     gene            complement(10247..10822)
                     /locus_tag="PK754_06450"
     CDS             complement(10247..10822)
                     /locus_tag="PK754_06450"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNB09178.1"
                     /translation="MAFKPSQRRSVKAESTELDLRPIMNMMCILIPLLLKCSEFVKMT
                     YLELNLPPLSGGGGGGGADDKPEKEKPKIGLKLVVTEKGMTIAGNSIVLAGEGGTTGP
                     TLPKLADGKYDFRGLEQKMKDIVKQINGKGFEDERTIIITAEDVIPYQAIVTAMDIIT
                     NSEFKEFSDPSSNQKVKQPWFANIGVGKIII"
     gene            complement(10908..11693)
                     /locus_tag="PK754_06455"
     CDS             complement(10908..11693)
                     /locus_tag="PK754_06455"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNB09179.1"
                     /translation="MKKNILAKAAGLTVTAQMMASSLFAQDGGAAAPQESQSQVAIFF
                     DAFFQSFKWEEAGSTYMWIIAFCGVFSVAIMAERLYFIFVRSNVDANKFMAEVRKLVA
                     GGNLKKAIALCETAKDKALPSVVLAGLKRAAEKEGGPLDFRAIQNAVDEGTLEIIPKL
                     SERSGYLAMLSNVSTLFGLMGTVYGLILAFAAVGNPAMPEDQKALFLAKGISAAMQTT
                     IWGLSVAIPCIIVYTLINTKTGKIIDEMDEHMVKLINLMTGNK"
BASE COUNT         3024 a         2999 c         2590 g         3155 t
ORIGIN      
        1 ggctttggtt ttgatatgcc ttcaagcgtg gaaatttttc ggcgataact tgccaaaaag
       61 tttctgcatc cgcggaagcc ggtaatacaa ttaccgcctc ctcgcatccg gccgcttcac
      121 ggcacatcgc aaaaaattta acgcgtacat tgatcatgtt catcattcca ctgaaatctg
      181 agttgccgcc gtcgttccga aaatctccgg atgatacatc gcttcggctt tggtagccgg
      241 cagcgcaaac gtaccgtagg tcgtcgcacg cacgtaatag acaaacttct gtgtcccgtt
      301 ttcaaaatag tcggcaaaag ccacgacgcg atcatcgcgc atttccgaac gtgtaaatcc
      361 accatactcc caccaacgat tttcggtccc actgccgtac gaagatgccg aagttgccaa
      421 atccgtgttg atcgcttcaa accccgccgg caacggatca tccaacacca catattggcg
      481 tgcttgcgcc gtactgaccg tcaatgtgat tttataaatt ttaccggctt tataacgtcc
      541 gtccgtgacg acgtgacccg aagcgtcttg tatttctttg ctcacccgca atccctcatc
      601 gcgtgccgga gcattaccgc ctgaacgcgg cgcataagtc atacgcgcat tgtaatataa
      661 acgccccgcg cctgttttgg ttatatcaag cagcaatgct ttaccgctcg gaaattcggc
      721 taaagccgta aaggatgtgt gactctgtgt cgtccggccc tgatacattt gagagagtaa
      781 ttgacgccct gccaaacgca ccgttgcttt gaaatccgga tccacgtctt caaaatttct
      841 gaaatacgta cccagcgcgt aaaacacata caagttttcc tgcgtcgtac gccagcgtcc
      901 ttgtttacgt tcttcaagca tatatttcac tactttttca gcccaaggta cgttggtgcc
      961 gtccagttcg ataaacgttt gcaatatcgc cgcggtggtg cgcatcggcg aatggaatga
     1021 ccattccgaa ccgggcaaat tcggctcttc aaaatgcgcc gtcgtgggat tcattttaat
     1081 cgcattcatc aaatcacgac gcaatgtttc ggtcatggct ttatgaccgc cgcctttgac
     1141 aatcgcttgc aaaagcattg cgcgtgcaaa caaaggtaat tcggaacggc gctggaataa
     1201 cagttcggcg ctggctgcat catacacatc gtgatcggcc aatacagcca atgcaaacgc
     1261 attggtattg tgccaataaa aaacaccata gcggtctacc ggattattac gaacaagctt
     1321 tttcaaatat tctacgccgt tgtccatgat ttctttgggt acatcataac cgatagcttt
     1381 cgccttggtc aaagcaaaca tcgcatacac gctgacatag ggcgatacga tatggtcgcc
     1441 ggtccaatac gaaaaaccac cgctgtactt ttgaaatttg ggaatctcat tcaggtattc
     1501 tttaaccgcc gcatcgaccg attttgtttt gtcttcaaac ggagtgagac caaatgccgt
     1561 caccacgtcg ccaaaaagaa tgaccggtaa aacacgggag gtgcgttgtt cgaggcagcc
     1621 gtaggggtat tcgaaaagat attttacact accgtcaagg tcaaccaatg ccgtagacga
     1681 agttttgatt tcaaaacttc ccgtgttttc aaaaatcgaa ccaggaatga tcacttgttc
     1741 ctgcgttttg tccgtcgtac tgccggcaag cgccacggtt tccgttggta cgggcatacg
     1801 caccggtatt gttatttcta cgccatcgct aaactgccgc atctgtgcag taaaactaaa
     1861 tttcgccggc ccggtcttaa gcgcacggta ttggaaacgc acttcttggg atgcgccggg
     1921 ttgcagctcc accgtttttt cgatggcacc gatgatttcg acgccttccg cctgtgcgcg
     1981 tatcgtcgct gtccccgctt gcgtggtgtt attgtgtagc accgcgccgg cttccacggc
     2041 atcatctatg cgggcaaaac gcggtagtgc aggccgaagc atgagatctt tgctgaccgt
     2101 aatggaatga tcgtcactgc caaaacgaga atcgcgggta tgggccaccg caaatatgcg
     2161 aaacatagta aggttgtccg gcagttgtac acgtactcgc gccttaccgc gcgcatcggt
     2221 gatcacggaa ggattccagt acacacagga tttgaaatct ttgcgcatgg caatgcccgg
     2281 catacttcgc tcttcaccgc cgccaccgcc gacaccgccc ttttctttaa gatcgcgctt
     2341 taccagttga tccaaaataa atcgccgcga ttcggtcgta gcaacctgca actcacgttg
     2401 tttgtaaaaa tgtgaaaaca catccggcgt ttgatatccc accagattga gtacgccgac
     2461 atcttcgaca taaagcgtca cttccgcaat ttgaccatgc cctgacttgt ccgccacttc
     2521 gacgtccaca tccatccatt caccgggttg aaatttattt ttcggtgttt gaattttcac
     2581 ggccagtttg ttagccgacg gatccacaga aaggttgata tagccgattt taaaagacgg
     2641 ctttcctatg tcttcatttt gatcgggcac aggttcagcg gttcgccctt gtaaaagcgt
     2701 caccgacaca aacgcattgg gcgccatctc ggccgtgatc ggaatttcga ccgaagcggc
     2761 attacctttg agttccgtca tgaaatgacg aataatacct tcgcgttcta cactcaccag
     2821 agcacgcgcg cgctcataag gcgacttgac gagtattttg gctttttcac cgactttata
     2881 ttggctttta ttcgcaacca aatcaatccg gtcgccatca ttgagtttcc atccggcata
     2941 ggattcgccg gtcacataaa cgtaggattc tgaaatgatc gtattgtcgt atggatcgct
     3001 cgaagtcgct ctcaagatat aataacccgt cgaggccgga ttcaattttt gcgtgacgat
     3061 ctccccgcgc gagcgtactg taaaaacctc ttccgttgta tcgcgctgag aagacgtcca
     3121 ccggtacgaa ccgtcatacg atttttcccg aacggaatgc cattcacggc gaatcaattc
     3181 caattcaatt tttttatcgg ccgttttttt tccatcaggc gttacggcta cgacatccac
     3241 cgacaagggt tcaccccatg tcataaacgt cgttgatggt ttgaggccta tataaaatgc
     3301 gccttgatgc acgcgcaccg ttgtccggcc gctgacatac tgcctgtttt tatcttctac
     3361 cgtaccttcg atgaccagtt ccgaagtttc ggaaacaccg ccggcattaa gcacaacgtc
     3421 ggcatccata aggccatgtt gatccaatgt cccggaggag ctcgcaagca ccgtttgata
     3481 cgcaccacgc ccgtaatacc cgtcgtaatc gtacatttta ccaaagaaat acccgtcata
     3541 cccttccggt tggaatgagc tggcattacg cgtaatactc cagttcatgg atgcattggc
     3601 cataggcgca ccaaaaagat agtgcgcatc aaaattaagt ttgagcgtct cactccatac
     3661 gtactcggat ttaaccgaat tgatggtcac cgcactttcg acaggacgaa actcctgcac
     3721 ctggaacgat tcgccggaga gttctttatt tttttccgac gtatacaaac gcgcataata
     3781 ataaccgagc ggcgcgtttt caggaacggt aaattcgaaa tccgttcctc caaacggatt
     3841 gactacgatg tcttgggcat acacttcatc gttggacgga ttaaaaatac gagctttcag
     3901 attgagtccc gtcggtacgg accacgcgcc tttttgtaag cgacgcagca ttgctttaaa
     3961 ttgcacttta tcgcccggcc tgtagatgcc ttgattggta aataacttag acgaaatttc
     4021 gtcttcggcc gactgaacat accacgcata tggaatatta aagcgccata aatcaatttt
     4081 attatccgag cgcgcgtagg ctgtttgatc accacgcgtt gcaaataccc acagcctcgg
     4141 ttcgttgtat gtatctatcg gttggagacc aaaaccgctc cagccctgtg tttccgctac
     4201 gccatccgaa ttggtatagc cactccacaa cagattaccc atatcgtcgc gtatctgcac
     4261 aaaggcacgt gcagcacggc cgtctttgag ctggctgacg gcaataacgc ttttatcgcg
     4321 ggcaaatttt gccgtaatgc cgatatccgt gacttgtatc agtgcgcgtt gatagtcatt
     4381 ccatgtatca tgcttcatct gtacgaagac gaaacccgac ggttgggagg ccaacgccgg
     4441 atccaatgcg agcggataat attgtgtcgc attacgcggc agatcaggac gttcagatat
     4501 cgtatgtgcc caacgattaa aaccgttatc gcgatataaa ccctctttgg ctaaaccaat
     4561 gacttcctct tgggtgatag cggccatttg aatatcaata ttgcccggat tgatcatcgc
     4621 cacattcaga tcgtggcgca gatacgattc taaaacatgt tctccggttt caaaataaac
     4681 cgacgacgcg taatcgcccg ttctgaaatt ctgccgaacc gtaactccca aagcattatc
     4741 aaaagcatct tttatctcgg gatcaatggt aatcgtataa tccgtttgcg gctgaagttg
     4801 cgcatagata ctcgcaggat actccgacga ccacgatgga tatatttcat tgatctcggt
     4861 atgcggtgtg atcgtaagat gcgatttgag ttcatcggca tctatgcgat tggtgaactt
     4921 tagttgaaaa ggactattgg gatattccgg cggaatcaat tccaaaaact ccaaaggccc
     4981 gtaggtcgaa atgtcatgcg tcacgcgctc ggatgaaccc agtacacctt cgcgtccttt
     5041 gaggccggat gcgatctgca attgataact cgtctgcatg cgcaacgatg tgatcggttt
     5101 tacgcgcaca atgcgctcaa cgggtgatcc ggcaacctgg tcataaccgt agttgtaata
     5161 cgtcagtaag tccgcgctat cggccggttc gatgctaacc ggtatattgg cgccgtcctg
     5221 aatacgaatg aggcgtatct tttccttggc ggaatggata tctatcggtt gattaaatgt
     5281 cagcatgatt ttcggattga gcgtagtgcc attcatctcc gaaatattat gactgatcaa
     5341 tttcggtcgc atggtttcaa acgaaaaaac atacggcgaa gacatcgcgc tgccatcgag
     5401 agcacgcgtg cctgccggta tggttacggt aaatcgtgta ttcagtggga gtttttcgga
     5461 cgggataaaa gacaatgtat tcgttcccat ccagcggaat ttgccgttta ctgaaggact
     5521 tatttccagc ggcccgtcac cgtcgccttc gggcaaagcg ctcaacgcca ccatcggttt
     5581 tgcgaaaatc acaacaacgg cataaaactg ctgaatatct gcgaccgttc cttgcggtgt
     5641 tgccgacaat acaaagtccc cggaaccgaa aaccgtttca ggcataggtg tggcttgcat
     5701 ggcgtccggt gcaatacaac cattgaggtg tatcgaagat aaaaaaacca ccgatatcga
     5761 taacaacaga cgtatcattt ttttcacttt agagcccatg accatctccc tatttcccat
     5821 tatttgctgt tacattggaa cgatatttta ttttaagcac aatcaaccaa agattcaatg
     5881 cgatggaaac gatattagcc ataatgataa ctaagtcatc acgcattacc ccatagagca
     5941 accatccgct catcccgatc aataaaacga ccggctgagc aatagccaca tcttcaactt
     6001 ttttagtacg aagacttttg atcacttgcg gtagaaaacc aaacgtagtt agaattccag
     6061 aaaccaaacc tacgatcatt gtatccatga agaccattca atcatgtttt gagttgagta
     6121 ttttttatat acattacatt tcaataactt atctgaataa ggataaatct tttatgaaag
     6181 acatttcagg ggcgattaat aacctgctga acaaattcaa aatttgggtg ctgcttggcg
     6241 gtgttgtaat cataacgatc gcttttacgg ccccccaatt cctgtttatt ttgatattcc
     6301 ctctattctt cggattattt ttctacatat tcaaaagctt cgtacaaccc atgatgcgca
     6361 agatggatgc tcaatttggc atcaagcttc ctcctgaatt cgatgagagc caagaaacaa
     6421 ctcgcgacat ttcgcgttgg gatgcaatac tatcgtgcgc tcaatccaaa ggcggccgtc
     6481 tgaccgcggc cgatgtttcg atttatttta atgtggatct gcagacggcg gaggtacgtc
     6541 ttgatcaatg cgtggaatca ggacatgcgg atctatatgt aagccagtcc ggcgttaaag
     6601 tatatcaatt ctctcacatt atcagcgacg acgaaaaaaa acgtgctgag cctgtataac
     6661 ttatttttaa agcgcatggt tctataccca tgcgctttaa cgtatccgat tattctgctg
     6721 ttcgtatatg cagttctttc agttgtcgtg catctacggg cgaaggcgat tgatccatca
     6781 gtgaaacggc gctattggtt ttaggaaatg cgatcacatc gcggatggat ttccgtgcgg
     6841 ccagaatcgc cgtcaaacga tcgaatccca atgcgatacc gccatgcggg ggtacgccat
     6901 attcaaatgc gcgtagtaaa aatccgaatt tggtatcgcg atcttcctgt gtaagcccca
     6961 acgcgtcaaa cattttattt tgtacatcgc gactgtgaat acggatactc ccgccgccga
     7021 cttccgaacc gttgagtacc agatcatacg cttttgcgcg cacggcgccg ggttccgaat
     7081 ccatcttcga aagatcttca tcaaacggcg acgtaaacgg gtggtgcatc gcattccagc
     7141 gtttttcttc ttcatcgtac tcgaaaagcg gaaagtcaac aacccaaaac atcgcatatg
     7201 catcgtcacg tacgagtttc aaatcctcgg cgagtttcaa acgcagcgcg cccaatgcgg
     7261 actgtacgat ttgttctttg cctgcggcga tcaaaagcaa atcgccctct ttagcgccgg
     7321 ccacttcggc catccggcgc aatttatctt cggtgaaaaa tttaccgata gatgaagtta
     7381 tgcccgtcgc ttcaactttg accgtcgcta aacccaggag gccgtgtttt tttacaaatt
     7441 ccgtcaactc atctattttt ttgcgcgaaa aagaggaggc ttgcgattcc acacaaagta
     7501 gagcgatcgc gccgtctttt tgatcaagta catcgttgaa aaccttgaac tccgaaccgc
     7561 gcacacattc ttcgatatac gtgatgggca taccaaaacg cagatccggc ttatcgctgc
     7621 cgtacattcg catcgcttca aaatacggca tacgacgcaa cggcatcgta aggtctatgt
     7681 ctttgagttc cttaaaaatt ttgaacatca gacgttcaat catgcccagt atgtcttcgc
     7741 gttcgacgaa cgacatttcc acgtccacct gcgtaaattc cggctgacgg tcggcgcgta
     7801 agtcttcatc acggaaacac ttgacgattt gaaaatagcg atccatgccc gacaccatca
     7861 gaagctgttt gtaaatctgc ggtgactgcg gcaaggcata aaattttccg gcgtggacgc
     7921 ggctgggcac aagataatcc cgcgcgcctt ccggcgtgct tttcatcagt acgggtgtct
     7981 cgacttcgac gaattgattt tcggtaaaat aattgcgtac gatttgcgcc agacgatgcc
     8041 tcagtacaag tgtttcctgc atatatgaac gccgcaaatc aaggtaacgg tactccaatc
     8101 gcagatcttc atctactttg aggttttcat cattgatttc aaatggcggt gttttggcac
     8161 ggctcatgat agtcagcgat tccgtgatca cttcgatttc accggtagga atattcttat
     8221 tcaccgattc ccgaggtgtg acgacgccta aaactttgat aacaaactca ctgcgaagct
     8281 cttttgcttt ttcgtgggtt tcagggaatt tatccggatt aaaaacgatc tgggttaaac
     8341 cataacgatc acgcaggtct ataaaaatga cctgtcccaa atcgcggcga cggtgaatcc
     8401 atccgatcaa ctttactttt tgcccggcat ttgcggcacg cagttcaccg catgtcgtac
     8461 tgcggtgagc cgtattcata atagcgctca tactttctct gatttccgat ctatcttgat
     8521 aattaaatat aaaaaaaacc gccacgcttg gggcgtgacg gttttatcac aatgtacgat
     8581 aatgcttact tcgtgaagac gaacgtctgg ttaaccgtta cgacgccttt tttaccgcgc
     8641 gggaaagtcc acccgcggat tttattggtt acgcaattag cgacggcatc gccaacggta
     8701 ttttgcgtaa cactcacacc ggaaacgcga cccgcttgat tgattttaat cgaaacggat
     8761 aacttacctt taagatccgg tgattttttg agttcttttt ggtaacacgc attgacggag
     8821 gttgcgtttt ggtcaataac gccttggatt tctgccgctg acgcaccttg tccgccaccg
     8881 gttcctgaga tcgaagcttt accgacaccg ataagaccgc taccggcgct accggccgct
     8941 acagacaatg cgcctgcacc gccaagctca gccgtgaggt ccgaaatgct tccggttccg
     9001 ccggctacac cgcccgcgcc aagcacggta cgatcaccgc caccaccggt cgcttcaccg
     9061 atacccccga cttgacccaa cacttcatca agattaccgc tgccgccgga gaaatcaaca
     9121 gccgcataac gaccgccacc accggcgcca accgcacccg caacagccaa aacacctcga
     9181 ccagcagccg cttttgcggc cgcaccggca cgtgctgctg cagcggcttt agccgcggcg
     9241 gcgcgcgttg cacgaccctg agcattcgtg ggcgcttgct gacgattgat ctgcgcactg
     9301 ggaacagatg atgtagctgt ttcttcagtt ttttcttctt cttttggctt atccaactct
     9361 tcaagaagcg cggcgtcaac tttgttgatt ttcgcaatag ctttcaaaag cttattatca
     9421 agcacagtcg gtttgggacg cggtggttgc atcgccatat agatcaacgg cgcatatacg
     9481 atcaccaaag aggcaaagaa aataccgagc atgcgtttat caaacgcaga ccagtaactt
     9541 ttctgaaact ctttaggaaa cgcaacgatt cgaatatcgc gtgccgcgcc agctgcgcgt
     9601 gcaccgcccg gaggggctgc cgcagcttta gtatttcgtt ttactgaatt cattccggac
     9661 tccttctcga gtcaattcac aaaattaaat tgttttatta gatcacatca tttttgttgg
     9721 ccaatgcgcc taacaaacga agattggaaa actgattacg accgcaagtt acaacgactt
     9781 tcatcagtac gttgaaaggt gtacgatcat cgcaaaccac aatcacctca cgcttaaatt
     9841 caataccaaa tcgcgcttcc aaatccaagg ctttattggc atcttcttca agcttttcag
     9901 caagctgtgg aatcaaaaat gaattctttt gcgccagtac cgtttccgtt tcggctattg
     9961 catctttgtt gtaaaaaatg tgtgcctgag aaacgttgat gagcaacatc ttcttaggtt
    10021 tgtttttggt aacgattttc ggcggttgca taccgtccga cttcgtagcc aaagcgccgg
    10081 aagtggaaaa gttcataaga aggaagagca acatgatagt cagaatgtcc atcatggagt
    10141 tcatattcag cgtaccttct tcgctacgct tattcttttt tgctgcagat ggtttaaatg
    10201 ccatgtaaaa tattccttta ggaaactaaa tttcggcgcc tatggtttag ataatgatct
    10261 taccgacacc gatattggca aaccacggtt gttttacttt ttgattactg ctcggatccg
    10321 aaaactcttt gaattcggag ttggtgatga tatccatcgc ggtgacgatg gcctgatacg
    10381 gaattacatc ctctgccgta atgataatgg ttcgctcatc ttcaaaaccc ttaccgttga
    10441 tttgtttgac gatatccttc attttctgtt caagacctct gaagtcgtat ttaccgtccg
    10501 ccaacttagg aagcgtcgga cccgtagtac cgccttcacc agccaaaacg atcgaatttc
    10561 cggcaatcgt catacctttt tcggtaacaa ctagcttaag cccgattttc ggtttttctt
    10621 tttcaggctt atcatccgcg ccgccaccac ctccaccacc gctcagcggg ggaaggttta
    10681 gctccaaata agtcatttta acgaattcgg aacatttgag aagcaacgga atcaagatac
    10741 acatcatgtt catgatgggc cgcagatcaa gctctgtgct ctcggcctta acactccgtc
    10801 tttgagaggg tttaaatgcc attgtaaaac ccttttaaaa ttataaaagt tctaataaat
    10861 cttaggtgtt gaaatcaaag cgataatcac tcgctaagat actgtaatta tttgttacca
    10921 gtcatcaaat tgatgagttt gaccatgtgt tcatccattt catcaatgat cttaccggtc
    10981 ttggtattga tcaatgtgta aacgatgata cagggaatag caacagaaag accccagatc
    11041 gtcgtttgca tagcggcaga gatacctttc gcgaggaaca acgctttttg atcttcaggc
    11101 atggcagggt tacctacggc ggcgaaagcg agaatcagac cgtacaccgt acccatcaga
    11161 ccgaaaaggg tcgaaacgtt ggataacata gcgaggtaac cggagcgttc cgagagtttt
    11221 ggaatgattt cgagtgtacc ttcgtcaacg gcattttgaa tggcacgaaa atctaacgga
    11281 ccgccttctt tttcagccgc acgtttcaga ccggcaagca caaccgacgg gagcgcttta
    11341 tcttttgctg tttcgcaaag cgcaatcgct tttttgaggt ttccaccggc aaccagctta
    11401 cgaacttcag ccatgaattt gttggcatct acattggaac gcacaaaaat aaaataaaga
    11461 cgttccgcca tgatggccac ggaaaaaaca ccgcaaaacg caatgatcca catgtaagta
    11521 gaacctgctt cttcccattt gaatgactgg aaaaatgcat caaagaaaat ggctacctgt
    11581 gattgcgatt cttgaggagc cgcagcgccg ccgtcttggg cgaaaagcga agaagccatc
    11641 atttgggctg tcactgtcaa gcctgctgcc ttagccaaga tgttcttttt catgaatact
    11701 aacctccggt tatgtttcag ggtactttaa tgaggaatgt ataaaaaatt tcgtgattca
    11761 gacttaga
//
LOCUS       DAOQNQ010000073        11636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1969_length_11636_cov_5.257663, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000073 DAOQNQ010000000
VERSION     DAOQNQ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11636
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1969_length_11636_cov_5.257663"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..959)
                     /locus_tag="PK754_06460"
     CDS             complement(54..959)
                     /locus_tag="PK754_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNB09180.1"
                     /translation="MKRMLLIATVILMACDGGKSDEALILDLTDFPLPLAASTAAIYN
                     GELYVFGGSDNYNGDTTYQSIFKFNGSSWETVDTMFNDATWDGKVLIKENFAYYVSGW
                     PDISGALKRYNFTTKTWTGLDTGGIGSNWGTTAQIVGNKIYHFDSYGEVEVYDMNTNQ
                     WLSRNPNGLKNGSERGLTSVVHDGNIFVTGYGDTVLYKYHVDADSFSVESVMPRSITS
                     CVMVELENKIYFIGGSENDEEAYVYSDSYIYDITEQEWTHSSIALKTPSIYTAYVLSG
                     STVYVIGGINASGDGITDAYKISLK"
     gene            complement(1079..3691)
                     /locus_tag="PK754_06465"
     CDS             complement(1079..3691)
                     /locus_tag="PK754_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HNB09181.1"
                     /translation="MTMEEQRIIDARSHEKPWKKWGPYLAERQWGTVREDYSPYGTAW
                     EYLTHDQARSKTYRWGEDGIAGISDEKQILCFAIALWNKKDPILKERFFGVTGTEGNH
                     GEDVKEYYYYLDSTPTHSYMKMLYKYPLEAFPYGQLVEENRRRTKLEPEFELADTKIF
                     DNDNYMDVFVEYAKNTPEDILIRVTIHNRSGQKATVNLLPQIWFRNTWAWGYDSYKPR
                     LHAENHAIHVTHAELGAMTLFSKEKGELLFCENETNYSKLYNMPPGSGYYKDAFHEYL
                     IGNNRKAVNPDMEGTKAGINYDISVEAQNSVTVLLRLGPSRMNDPFSDFDTVFQNRIK
                     ECDEFYATLQKKLNNDDMCLIQRQALAGMLWSKQFYYFDVAQWLAGDPGQPSPPSERK
                     KGRNHAWTHLNNADILSMPDKWEYPWYAAWDLAFHCIPLAMVDIDFAKHQLTLLTHEW
                     YMHPNGQLPAYEWQFGDVNPPVHAWAAWRVYSVEKMKCGAGDLHFLKSIFHKLLLNFT
                     WWVNRKDSEGNNVFEGGFLGLDNIGVFDRSAELPTGGKLEQADGTSWMAMYAVNMMRI
                     ALELARFDKVYEDMATKFFEHFLLIAGAIADTGKGGGDLWDEEDQFFYDVLRAPGIGE
                     MRLKVRSMVGLTPIFAVEILDTQSLHEVPTFAKRLEWFLQHRPDLAKLVSRWQEPGIG
                     EKRLLSLLRGHRLKQLLKRMLDENEFLSDYGIRALSKFHQKNPYVFQSNGKSFTVNYQ
                     PGESESEVFGGNSNWRGPIWMPMNYMLIASLYKFHSYYGDDFLVEHPTGSGNYMTLTA
                     IADDLSKRLTRIFLRNDQGQRPIFGDIAKFQKDPFFKDYILFYEYFHGDNGCGVGASH
                     QTGWTGLIAKLLEA"
     gene            complement(3830..8083)
                     /gene="rpoC"
                     /locus_tag="PK754_06470"
     CDS             complement(3830..8083)
                     /gene="rpoC"
                     /locus_tag="PK754_06470"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNB09182.1"
                     /translation="MSIYLSNNSLSKDFSTITIGISSPDAILSRSYGEVTKAETINYR
                     SFKPEKDGLFCEKIFGPVKDWECHCGKYKRIRYKGIICDRCGVEVTLKAVRRERMGHI
                     SLAVPVVHIWFFKSLPSKIGSLLGLSIKQLEKIIYYENYIVVQPGKSGLKYKDIIPEE
                     ELYRVKEELGLDNENLPNTDPNKFIALIGGEAVKELLKRVDVEQICIDLRKVLRDPST
                     SIQKRSDALRQLRVADAFRQSEGAIENRPEWMVLDVVPVIPPELRPLVPLEGGRFATS
                     DLNDLYRRVIIRNNRLRKLIEIRAPEVILRNEKRMLQESVDSLFDNSRKTAAVRSDSN
                     RPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKLHECGLPKEMAVELFKP
                     FIIRKLVERGYVKTVKSAKRMVDKHGAEVWEILEKIVSDHPVMLNRAPTLHRLGIQAF
                     QPVLVEGRAIQVHPLVCAAFNADFDGDQMAVHIPLSYEAQIEARMLMLSSHNIIQPSS
                     GRPIAIPSQDIVLGNYYLTKAKSGARGEGMRFSSIAEVRQAFDNGVAELHAKIKLRYV
                     KGEGKTATVAYIDTTVGRSLFNEIIPKEMGFVNELMTKKKIEETVSRMHRAAGNLKTV
                     EFLDNLKMLGFMTATRAGVSIGIEDVLTPPNKEGVLKQASSQVEKVQKTYRMGLMTDG
                     ERYNKIIDIWTHASNDVAASMYESTQTDRGGFNPIFMMMDSGARGSRDQIKQLGGMRG
                     LMSKPQKSMTGQVGEIIENPITASFKEGLSVWEYFISTHGARKGLADTALKTADAGYL
                     TRRLVDVAQDVIITEDDCGTILGVKTMAIKEGEEIKESLGERCLGRVAADDVIDPSTG
                     KVAIEAGELIDEAKAEMLNNSAIEMVEIRSVLTCESKRGVCAKCYGRNLATGNVVNMG
                     EAVGVMAAQSIGEPGTQLTLRTFHIGGVAARVGELPQALSKSDGKVVFAPGMKMLERQ
                     TDGIVISLSRNGRIELLDEDRLVYAYKVPYGAELSVHDGQKVKKDDLIMTWDPYNTPI
                     ISEHDGIVHYEGLKEGVTCREEADEATGMKHLVVIESRDKKMTPSVVIKKGREVLETH
                     ILPTRAYLVIKDGEEVHAGEAVAKIPREMAKTKDITGGLPRVQELFEARRPKDSSAVA
                     EIDGTVKIGKISRGYREIMIEGKDGESKSYKIPFGKHLIVHEGDYVIAGEQMSDGSVA
                     PHDILRIKGPAEVQRYLVNEIQEVYRTQGVKINDKHIEVIVRQMLQRVRIQDPGDTYF
                     LQDDQVDRLRFNEENQGIRDKVVVSDKGDSNLKEGQLISRIEIEKICKKLEKKRPPQY
                     REAQPATAEPLLLGITQASLSTESFISAASFQETTKVLADAAINGKTDYLLGLKENVI
                     MGRLIPAGTGLKRYQDIAVTSKDVEEEAASGKKTKSAKK"
     gene            complement(8148..>11636)
                     /gene="rpoB"
                     /locus_tag="PK754_06475"
     CDS             complement(8148..>11636)
                     /gene="rpoB"
                     /locus_tag="PK754_06475"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNB09183.1"
                     /translation="VSFSVPLKATLRLISKKSADDKHPEIIEQDVYLGNLPYITEKGT
                     FVINGAERVIVSQLHRSPGVFFDESLHPNGTKMFSARVIPFRGSWIEFTTDINDVMYV
                     FIDRKKKFPVTTFLRALGYSSDRDMLKLFNIIDEVEISKIDGDKIMGRKLALDIVNEE
                     TGEIIAERETELTEETFAKLKRSKIKKIDLIKAEEVIPEVIANTIRKDVTKDEKEALE
                     TIYRQLRSGDAPDLETARGLLERQFFNSKRYDLGDVGRNRINKRLSLNIPESVTVLTK
                     DDIIAIIKALIELRDGIRQTDDIDHLGNRRVKTVGEQLAGQFSVALTRMTRTIKERMN
                     LRDNENLTPQDLVNARTVSSVINTFFGTSQLSQFMDQTNPLSELTHKRRLSALGPGGL
                     TRERAGFEVRDVHYTHYGRLCPVETPEGPNIGLISSLCLFARVNRYGFIETPYRKVVK
                     GRVQENIEFLIASDEDGMTIAQANEPIDKKGSFDNEFVKARKEGDFPVVTPDEIQFMD
                     ISPAQIVSPAAALIPFLEHDDANRALMGSNMQRQAVPLLVPEAPIVGTGLERTVAVDS
                     RSVIISDIDGVIEDVSATEITIRKKTMGGKKGKDSVSDTMTYRLLKFFRTNQDTCINQ
                     RPIVSVGDEVKAGQIIADGCSTERGDLALGRNVLAAFMPWRGYNFEDAIILSERVIRD
                     DVFTSIHIEEYEIQVRDTKRGEEELTREIPNVSEEATKDLDENGVIRVGAEVLAGDIL
                     VGKVTPKGETDPTPEEKLLKAIFGDKAGDVKDASLKAPPGMKGIVISTKLFSRKKKDT
                     ESKKEDKKKLEELENELKKQVRRLKSNLRDELTKLLVGKVSAGVVYTDGSEFLKKGKK
                     FTEDSFKNLDPAEINLQESRWTDDEETNEMANFLFDNFERNAEEVETEYNSEKFKVQV
                     GDELPPGIVQLAKVQVAKKRKISVGDKMAGRHGNKGVVSKIVPTEDMPFLSDGTPVDV
                     ILNPLGVPSRMNLGQIFETSLGWAGKILGKHYETNVFDGAKISDVKNELRLAGLPDHG
                     KVRLTDGRTGDRFDQETMVGYIYMMKLNHLVDDKIHSRSIGPYSLITQQPLGGKAQFG
                     GQRFGEMEVWALEAYGAAYILQEILTYKSDDVGGRAKVYEAIVKGDNLPEPGVPESFN
                     VLVRELQGLGLEVKIE"
BASE COUNT         3029 a         2859 c         2439 g         3309 t
ORIGIN      
        1 caaagctaaa gcccaaatcc aataattttt tttaataaac cgcataggac ctcctatttt
       61 agagatattt tatatgcatc agtaatacca tcaccactgg cattgatacc gccaatcaca
      121 tacaccgtac tcccggataa cacataagcg gtataaatgg aaggagtttt taatgcaata
      181 gagctgtgag tccattcctg ttctgttata tcgtaaatat atgaatctga atacacatag
      241 gcttcttcat cattttcgct accgcctata aaatagattt tgttttctaa ctcaaccatc
      301 acgcaagatg taattgaacg cggcattacg gattctactg aaaacgaatc ggcatcaaca
      361 tgatatttat acaacaccgt atccccgtaa cctgttacaa agatatttcc gtcatgtacc
      421 accgaagtca aacctctctc cgatccgttt tttaagccgt tcggattacg tgataaccat
      481 tgattggtat tcatatcata aacttcgact tcaccgtaag aatcaaagtg ataaatctta
      541 ttgcccacga tctgagctgt tgttccccaa ttcgaaccaa taccgccggt gtcaaggccg
      601 gtccatgttt ttgtcgtaaa attataacgt tttagcgcgc ctgaaatatc cggccatcct
      661 gaaacgtaat aagcaaaatt ttcttttatc aaaacctttc cgtcccaagt agcgtcatta
      721 aacatcgtat ctaccgtttc ccaactactt ccattaaatt taaaaatgga ttgatacgtc
      781 gtatcgccat tgtagttatc actgcctccg aatacgtaca attcaccgtt gtatattgcc
      841 gctgtcgaag ccgctaaagg cagcggaaaa tcggtcaaat ctaggatcaa ggcctcatct
      901 gattttccac catcgcaagc cataagaata actgtggcaa tcaatagcat tcgtttcata
      961 gatttctccg ggataagtat ataaaaattt atttaagtta atgcatattc agcatacagg
     1021 acctgtcaaa aacggatatt aagctatttc gtacattacc aacaaataca gcctaaaatt
     1081 aagcctccaa aagttttgca atcaaccctg tccatcctgt ttggtgggag gcgccaacac
     1141 cacacccatt atcgccgtga aagtattcat aaaataaaat atagtctttg aaaaaaggat
     1201 ctttctgaaa tttcgctata tctccaaaaa tgggacgctg gccttgatca tttcttagaa
     1261 aaatccgagt taaccgtttt gacaaatcat ccgctatagc cgtcagcgtc atatagttac
     1321 ctgaacctgt tggatgttct accaagaaat catcgccata ataactatga aacttatata
     1381 acgaagctat aagcatatag ttcatcggca tccatatcgg gccgcgccaa ttactatttc
     1441 caccaaaaac ttccgattcc gactctccgg gttgataatt aaccgtaaac gatttgccat
     1501 tagattgaaa tacataagga tttttctgat gaaatttaga caatgcacgg atcccatagt
     1561 cagataaaaa ttcgttttca tctaacatcc gcttaagaag ttgtttgagg cgatgtcctc
     1621 gcaaaagaga taataaacgt ttttctccaa tacctggctc ttgccaacgc gaaacaagtt
     1681 tggccaaatc aggtctgtgc tggagaaacc attccaatcg ttttgcaaat gtaggcactt
     1741 cgtgtaaaga ttgtgtatct agaatttcga ctgcaaatat gggtgtaaga cccaccatcg
     1801 agcgcacttt gaggcgcatt tcaccaatac ccggtgctct tagaacatca taaaaaaatt
     1861 gatcttcttc atcccagagg tcaccgcccc ctttacctgt atctgctata gcgccggcta
     1921 taagcagaaa atgctcaaaa aacttggttg ccatgtcttc gtaaacttta tcaaaacgcg
     1981 ccaattccaa agctatacgc atcatattga cggcatacat cgccatccag ctcgtaccgt
     2041 ccgcttgttc tagctttcct cccgtaggta attcggcgct acggtcaaaa acaccgatat
     2101 tatccaaacc gaggaagccg ccttcaaaaa cgttattgcc ttcggaatct ttgcgattga
     2161 cccaccatgt gaaattgaga agtaatttgt gaaaaatgga ttttaaaaaa tgaagatccc
     2221 ccgccccgca tttcattttt tcaacactat atacacgcca ggcggcccag gcatgtacag
     2281 gggggttgac gtcaccaaat tgccattcgt acgccggcag ttgtccattg ggatgcatgt
     2341 accattcatg agtaagcagc gtcaattgat gcttggcgaa atcaatatcc accattgcta
     2401 acggaataca gtgaaacgcc aaatcccatg ccgcatacca gggatactcc catttgtcgg
     2461 gcatagatag gatatccgca ttattcaggt gggtccaagc atgattacgg cctttttttc
     2521 tttcgctagg cggtgaaggt tgtcccggat ctccggctaa ccactgtgct acgtcaaaat
     2581 aataaaactg tttactccac aacatgccgg ccaaagcttg tctctgaatt aaacacatgt
     2641 catcattatt caattttttt tgaagcgtag catagaattc atcgcattcc tttattcgat
     2701 tttgaaaaac agtatcaaaa tcagaaaacg gatcgttcat tcgtgacggg cctaaccgta
     2761 gaagaacggt aaccgaattt tgggcctcca ccgaaatatc ataattgatt cccgctttcg
     2821 ttccctccat atccgggttg acggcctttc ggttattacc aattaagtat tcgtgaaatg
     2881 cgtctttgta atatcccgaa cccggcggca tattatataa cttagaataa ttggtttcgt
     2941 tttcacaaaa aagaagctcc cccttttcct tcgaaaataa cgtcattgct ccaagttcag
     3001 cgtgggtcac atgtatggca tgattttcag catgaagccg cggcttataa ctgtcgtacc
     3061 cccatgccca agtattacga aaccaaatct ggggtaataa attgaccgtt gctttttgcc
     3121 cgctgcgatt gtgaatggtg acacgaatga gaatatcttc cggtgtattt ttggcgtatt
     3181 caacgaatac atccatataa ttatcgttat caaatatttt agtatcggca agttcaaatt
     3241 ccggttcgag tttagtgcgt cttcgattct cttccacaag ttggccgtac gggaaagctt
     3301 caagtggata cttatacaac attttcatat acgaatgtgt cggcgtactg tcgagataat
     3361 aatagtactc ttttacatct tcgccgtggt ttccttcggt gcccgttacg ccaaaaaatc
     3421 gttctttaag tataggatct ttcttattcc acagcgctat ggcaaaacat aaaatttgtt
     3481 tctcatccga gatgccggca ataccgtctt ctccccaacg atatgtttta gaacgcgcct
     3541 gatcatgagt gagatattcc catgccgtac catagggact gtaatcttcg cgaacggtac
     3601 cccattggcg ttccgcgaga taaggccccc attttttcca gggtttttca tgtgatcttg
     3661 cgtcaatgat acgctgctct tccattgtca tatgagggac tctttcgtac aaagattggt
     3721 tctatagacg gcgtgttatg ccaatgtaat acatagtttc cgacagaaca aaaaaaaagt
     3781 gatgctgtca tttaacagca tcactttata tgatcgtatt tttatacgac tatttttttg
     3841 cggacttagt tttctttccg cttgccgctt cttcttcgac atcctttgaa gtaacagcaa
     3901 tgtcttggta acgcttaagt ccggttccgg cagggattaa tcggcccatg atcacgtttt
     3961 ctttaagtcc gagcagataa tctgttttac cgttaatcgc tgcatccgca agcactttgg
     4021 tagtttcctg gaaggaagct gccgaaataa agctttcggt gctaagcgaa gcttgggtga
     4081 ttccaagcaa taacggttcg gccgtcgccg gctgcgcttc gcgatactga ggcgggcgtt
     4141 ttttctccag ttttttgcag atcttttcga tctcgattcg gctgataagt tgtccctctt
     4201 tgaggttgga atcaccttta tcagaaacca cgactttatc gcggatgcct tgattctcct
     4261 cattaaaacg aagacggtca acctgatcat cctggaggaa atacgtatcg cccggatcct
     4321 gaatacgaac gcgttgcaac atctgacgta cgatcacctc gatgtgttta tcgttgatct
     4381 tcacaccttg agtacggtac acttcctgaa tttcgttgac caagtagcgt tgtacttcgg
     4441 cagggccttt gatacgtaaa atatcatgcg gtgcgacaga cccatcggac atttgttcgc
     4501 cggcgatcac gtaatcgcct tcgtgtacga taaggtgctt accgaaagga attttgtaag
     4561 acttgctttc accgtctttt ccttcgatca tgatttcgcg gtaaccgcgg gagattttac
     4621 caattttgac cgtaccgtca atttcagcca cggcagacga atctttggga cggcgtgcct
     4681 caaagagttc ctgtacgcgc gggagaccgc cggtaatgtc ttttgttttc gccatttcac
     4741 gcgggatttt ggctaccgct tcaccggcat gcacttcttc cccatctttg ataacgaggt
     4801 aagcgcgagt cggtaaaatg tgggtctcca gaacttcgcg accttttttg atcacgacgc
     4861 tcggggtcat ctttttatcg cgggattcga taactacaag gtgcttcata ccggtagctt
     4921 catcggcttc ttcacggcat gtcacgcctt ctttcaatcc ttcataatga acaataccat
     4981 catgttcgga aataatcggc gtattgtacg gatcccatgt catgataaga tcgtcttttt
     5041 tgactttttg gccgtcatgt acgctcaatt ccgcaccgta gggaactttg tatgcgtata
     5101 ccaaacgatc ttcatcaagc agttcgatac gtccgttgcg gcttaacgaa atcacgatac
     5161 catcggtttg acgttcgagc attttcatac ctggagcgaa aacaacttta ccatccgatt
     5221 tagaaagcgc ttgcggtaat tcaccgacgc gtgcagccac accgccgatg tggaatgtac
     5281 gcagagtaag ctgtgtaccc ggttcaccga tggactgggc agccataaca ccgacggctt
     5341 cacccatatt tacaacgtta ccggttgcaa ggttacgtcc gtaacactta gcacatactc
     5401 cgcgtttgga ttcgcaagta agtacggaac gaatttcaac catttcgatg gctgaattat
     5461 tcagcatctc agctttggct tcatcaataa gttcaccggc ttcgatagcc actttaccgg
     5521 tcgaaggatc tatcacgtca tccgcagcaa cacggccgag gcagcgttca ccgagtgact
     5581 ctttgatttc ttcaccttct ttgatagcca tcgttttaac gccaaggatc gtgccacaat
     5641 cgtcttcagt tatgatcacg tcttgagcaa cgtccacaag acggcgggtc aaataaccgg
     5701 cgtcggctgt cttcaaagcc gtgtcggcaa gacctttacg cgcaccgtgc gttgagataa
     5761 agtactccca taccgacaaa ccttctttga agctggccgt gatcgggttt tcgatgattt
     5821 caccgacctg acccgtcata ctcttttgcg gtttggacat aagtccgcgc ataccaccga
     5881 gctgcttgat ctgatcacgc gaaccgcgag cgccggaatc catcatcatg aaaataggat
     5941 taaaaccgcc gcgatcggtt tgtgtacttt catacatcga agccgccaca tcattggatg
     6001 cgtgggtcca gatgtcgata attttattgt aacgctcacc gtcggtcatg agacccatac
     6061 ggtatgtttt ttgaactttt tcgacttgcg aagaggcttg tttgagtacg ccttctttat
     6121 tcggaggcgt taatacgtcc tcaataccga tagacacacc ggcacgcgtc gccgtcataa
     6181 aaccaagcat cttgaggtta tcaagaaatt caacggtctt aagatttcct gcagcgcgat
     6241 gcatacggga aacagtttct tcgattttct ttttcgtcat caattcattg acaaatccca
     6301 tttctttggg gatgatttca ttgaaaagag agcgacccac cgtcgtatca atatacgcca
     6361 ccgtagccgt tttgccttca cctttgacat aacgcaattt gattttggca tgcaattcgg
     6421 caacgccgtt atcaaatgcc tgacgaactt cggcaataga tgagaaacgc atcccttcac
     6481 cgcgtgcacc ggacttcgct ttagtcagat aatagttacc caaaacaata tcctgcgaag
     6541 gaattgctat cgggcgaccg ctggaaggct gaataatatt atggctcgaa agcatcagca
     6601 tacgcgcttc gatttgcgct tcgtaagaca gcgggatgtg aacggccatc tgatcgccgt
     6661 caaagtctgc gttaaatgcg gcgcaaacca gcgggtgcac ttggatcgca cggccttcga
     6721 cgagcaccgg ttgaaacgct tggataccaa ggcggtgcaa cgtaggagcg cggttaagca
     6781 ttacagggtg atcggaaacg atcttttcca atatctccca tacttccgca ccatgcttat
     6841 ccaccatgcg tttagctgac ttgacggttt ttacataacc gcgttcgacc agtttacgaa
     6901 taatgaacgg cttgaataat tcgacggcca tctctttggg caaaccacac tcatgaagtt
     6961 tgagttcagg tccgacgacg atcacggaac gaccggaata atcaacgcgt tttccgagca
     7021 agttttgacg gaaacgacct tgttttcctt tgagcatgtc cgacaaggat ttcaacggac
     7081 gattgctgtc gctgcgaacc gctgctgtct tacggctgtt atcaaaaagg gaatcaaccg
     7141 attcctgaag catgcgcttt tcgttgcgca aaatcacctc aggagcgcgg atttcgatca
     7201 atttacgtaa tcggttatta cgtatgatca cacgacgata taaatcattc agatcgcttg
     7261 tcgcaaaacg tccgccttca agcggtacga gcgggcgcaa ttcgggagga atgaccggca
     7321 ctacatcgag caccatccac tcagggcggt tttcgatcgc gccttcgctc tggcgaaatg
     7381 catcggccac gcgaagctga cgcaacgcat cggaacgttt ttgaatggat gtcgaaggat
     7441 cgcgcaacac tttacgtaag tcaatgcata tctgctcgac atcaacacgc ttgagcaatt
     7501 ctttgacggc ttccccgccg atcaacgcaa taaacttatt gggatcggtg ttcggcagat
     7561 tttcattatc caatccgagc tcttctttga cacgatacag ctcttcttca gggataatat
     7621 ctttgtactt gagaccgctt ttgccgggct gaacgacaat atagttttcg taataaatga
     7681 ttttttcaag ctgcttgatg ctcaggccaa gaagcgaacc gatcttgctc ggcaaggatt
     7741 tgaaaaacca aatgtgtacg acgggtacgg ccaacgaaat atgccccatg cgttcgcgac
     7801 gaacggcttt gagcgtcact tccacgccgc aacggtcgca gatgatgcct ttgtagcgaa
     7861 tacgcttgta tttaccacaa tggcattccc aatcctttac gggaccgaaa attttctcgc
     7921 aaaacaaacc atctttttcc ggcttgaatg agcggtaatt gatagtttcc gctttggtga
     7981 cttctccgta cgaacggctc aaaatcgcat ccggcgatga gataccgatc gtgatcgttg
     8041 aaaagtcttt tgacaaactg ttattggaca aatatatgga cacggttgat cctcctttat
     8101 cggagcgtta tgagttaatc taaattacaa caccacgtgc gttgcaatta ctcaattttt
     8161 acttcaaggc caaggccctg caattcgcgt acaagcacgt taaatgattc gggcacaccc
     8221 ggttcaggca aattatcgcc tttaacgata gcttcgtaca ctttcgcacg tccgccgaca
     8281 tcatcggatt tgtacgtgag aatttcttgc agaatgtaag ccgcgccgta ggcttcgagc
     8341 gcccacactt ccatttcacc aaaacgctga ccgccaaact gagccttacc tcccaagggc
     8401 tgttgcgtga tgagtgagta cggtccgatg gaacggctat ggatcttatc atcgacaagg
     8461 tggtttagtt tcatcatgta gatgtagccg accatggttt cctgatcgaa gcgatcaccg
     8521 gtacgaccgt ctgtcaagcg cactttaccg tgatccggta aaccggccag acgtaattcg
     8581 tttttcacat cggatatctt cgctccgtca aacacattgg tttcgtagtg tttgccgaga
     8641 atcttaccag cccaaccaag cgaagtttca aatatctgtc caagattcat acgcgaaggc
     8701 acaccgagcg gattgagaat cacgtcaaca ggcgtaccgt cgctcagaaa tggcatatct
     8761 tcggtgggaa cgatttttga cacgacgcct ttgtttccgt gacgtccggc catcttatcg
     8821 ccgaccgaga ttttgcgttt cttagcaact tggactttag ccaactgtac aattcccgga
     8881 ggcaactcat ccccaacctg gactttgaat ttctcggaat tgtattccgt ttcgacttct
     8941 tcggcattgc gttcaaagtt gtcaaataag aaattggcca tctcgtttgt ttcttcatca
     9001 tccgtccaac ggctttcctg caggttgatt tccgcagggt ccaaattttt gaaagaatct
     9061 tcggtaaatt ttttaccttt tttcaaaaat tcagaaccgt ccgtgtaaac aacgcctgcc
     9121 gatactttgc cgacaagcag tttggtcaac tcatcgcgca agttggattt cagacggcga
     9181 acttgttttt taagttcgtt ttcaagttct tcgagcttct tcttatcttc ttttttggac
     9241 tcagtatctt ttttcttacg gctgaaaagt ttggtcgaaa tgacaattcc cttcatgccc
     9301 ggaggcgctt tgagggatgc atccttgacg tcaccggctt tatctccgaa aatcgctttc
     9361 agaagttttt cttccggcgt aggatccgtt tcacctttag gtgttacttt tccgacaaga
     9421 atatcaccgg ccaatacttc tgcaccgaca cgaataacac cgttttcatc aagatccttc
     9481 gtcgcttctt cgctgacgtt gggaatttcg cgcgtaagtt cttcttcgcc gcgtttggta
     9541 tcacgtactt gtatttcgta ctcttcgatg tgtattgacg taaatacgtc atcacggatc
     9601 acgcgttcgc tcagaatgat agcatcttcg aagttataac cgcgccacgg catgaaggcc
     9661 gccaacacgt tacgaccgag agcaagatcg ccgcgttcgg tcgaacaacc gtcagcaata
     9721 atttgtccgg ctttcacttc atcacctaca gagacgatgg gacgttgatt gatacacgta
     9781 tcttgattgg tacggaaaaa cttcagtaac cgatacgtca tcgtatccga aacagagtct
     9841 ttacctttct ttccacccat ggttttctta cggatggtga tttctgttgc cgaaacatct
     9901 tcgatcacac cgtcaatatc cgaaatgata accgaacgtg aatccacagc cacggtgcgc
     9961 tcaagaccgg tacctacgat cggcgcttcc ggcacaagca acggtaccgc ttgacgttgc
    10021 atgttagatc ccatcagtgc gcggttggca tcatcgtgct caaggaatgg aatcaaagcg
    10081 gccgccggcg atacaatctg cgcaggcgaa atatccatga actggatttc atccggagtt
    10141 accaccggaa aatcaccttc tttacgcgct tttacgaatt cattgtcaaa agaacctttt
    10201 ttgtcaatcg gttcgttcgc ttgtgcgatc gtcatgccgt cttcgtcgct ggcaatcaga
    10261 aattcaatat tttcctgtac acgacctttg acgactttac gatagggcgt ttcaataaaa
    10321 ccgtaccgat tcactcgtgc aaaaaggcaa agcgaagaaa taagaccgat attcggtcct
    10381 tccggtgttt cgaccgggca aagacgaccg tagtgcgtat aatgcacgtc gcgcacttcg
    10441 aatcctgcgc gttcacgcgt aagaccgccc ggtccgagag ccgagagacg acgtttatgc
    10501 gtcaactcgg ataacgggtt cgtctgatcc ataaactgtg aaagctggct ggtaccgaaa
    10561 aacgtattga ttacgctgga taccgtacgc gcattgacga gatcttgtgg tgtcagattt
    10621 tcattatcgc gcaggttcat acgttctttg atcgtacgtg tcatacgcgt caaagctacg
    10681 ctgaattgtc cggcgagctg ctcaccgacg gttttcacac gacgatttcc aagatggtct
    10741 atatcgtcag tttggcgaat accgtcgcgc aattctatca atgctttgat gatagcgata
    10801 atatcatctt tagtaagaac cgtaaccgat tccggtatat tgaggctcag acgtttattg
    10861 atacgattac gtcctacatc accgagatca taacgtttgg aattaaaaaa ttgacgttcc
    10921 aaaagtccac gtgccgtttc caaatccggt gcatcgccgg agcgaagttg acggtaaatc
    10981 gtttccaaag cttctttttc atctttggta acgtctttac gaatcgtatt ggcgatcact
    11041 tccggaatga cttcttcggc tttgatcaaa tcaatttttt tgattttgct gcgtttgagc
    11101 ttggcaaaag tttcttcagt cagttcagtt tcgcgttcgg caataatttc gccggtttcc
    11161 tcattgacga tatctaacgc caatttacgg cccataatct tatcgccgtc aatcttggag
    11221 atttccactt cgtcaataat attaaaaagc ttgagcatgt cgcggtccga cgaatagccc
    11281 aaagcacgta aaaacgtcgt tacggggaat ttctttttac ggtcaataaa cacatacatg
    11341 acatcgttga tgtccgtcgt gaactctatc cacgaaccgc ggaaaggtat gacgcgcgcc
    11401 gaaaacatct tggttccgtt cggatgcagc gattcgtcaa aaaatacgcc cggggaacgg
    11461 tgaagctggc ttacgatgac gcgttctgca ccgttgataa caaaggtacc cttctccgtt
    11521 atgtacggaa ggttaccgag atacacatcc tgttcgatga tctcgggatg cttgtcatcc
    11581 gctgattttt tgctgatcag acgcaacgtc gctttcaaag gaacagaaaa cgaaac
//
LOCUS       DAOQNQ010000074        11596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1978_length_11596_cov_6.237588, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000074 DAOQNQ010000000
VERSION     DAOQNQ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11596
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1978_length_11596_cov_6.237588"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="PK754_06480"
     CDS             <1..1010
                     /locus_tag="PK754_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB09184.1"
                     /translation="ENWDIEAGLRYDYFDSPRDYQFVDPRLAAKYRINETTNLKASAG
                     RYHQYVHRIPRTFFASIWTTTDQYQKASEAYHFITGFEKTFDDVYQVEVETYYKKYNS
                     IYTLNRFLVTDMKPSYYDEQGNPIFTNTNGIFNHGNGNSIGLELMLRKDVGAFTGWVG
                     YSLARTEYNVEGKNHGQSFTPRHDRTHTINAVGNIDIRNGMRVIRGLETTKSNSRWTL
                     GFNFIYSSGQPITRPGSLYVITPTPDNGNPVIIEYPDDINNYRLPYYARLDVSLTYEK
                     QFKSWSIAPYLQIYNIGNRKNVWFIDYPYEIKEGGTIKPKVKTTSMFPMLPSIGINFT
                     F"
     gene            1023..1934
                     /locus_tag="PK754_06485"
     CDS             1023..1934
                     /locus_tag="PK754_06485"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HNB09185.1"
                     /translation="MNKFKFLFYIPLLLITACGEGKVSIDNINYKPKIVIRGLIFPGQ
                     PVSGISITRNLPIREQQLTLDQLIIADAKVVITNVSSTVTDTLVFDPITATYYDPEHN
                     FSVQYNTIYRLNVAAQIDGKNLNASSETKVPQQGFSIIEESSRLDSLHYRERDEKDSI
                     RTFTVRFERSVNAETYLMSMNALDADTSTYVYSPVNPYSDLKPSDVLKDLDDFKYSTQ
                     WSSLFENQPGQSEMSVFWFFLSFYGRYQMVVYAADENYSDFINTYDQVQDIDGNYDEP
                     VFHIDGDGIGVFGSAIADTVYFKVLKP"
     gene            complement(1968..2966)
                     /locus_tag="PK754_06490"
     CDS             complement(1968..2966)
                     /locus_tag="PK754_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNB09186.1"
                     /translation="MISLNDRLLHEDKPRRYAVGLDLGGTNLKYGIVSDRGEVIYQNV
                     IPVFADHGRDHVLQTMVDAAYECVMFGRKESFEVEGIGVGCPGTIDSDRGISLGPTPH
                     LPDWENAPIAEYISQKMNLPVFADNDANFMAFGEAMLGAGKQYKLVVGITLGTGVGGG
                     IIINGNIFHGSSYNGAELGHTIVEAHGRPCGCGNQGCLEQYAGGKYIVKEALSAIARG
                     DKSVLSSVENITPKTIFDFAQKNDPLCLNIVEQMVFYLGAGLSSIVHVLNPDIIIIGG
                     GLSEAGDAFVSRIHNEVIKRVMKPIKPKLQIVRAKLGNTAGLLGAGYYVLYKNRSK"
     gene            complement(2950..4506)
                     /locus_tag="PK754_06495"
     CDS             complement(2950..4506)
                     /locus_tag="PK754_06495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNB09187.1"
                     /translation="MPSSLFDLSAGFSVVDVETTGDKSSIGYVIEIGIVNVRDFKITD
                     TFHSLVKPPIPIPPFITKLTGLTDRDVSSAPVFADIAHKVEAMMEGHYFVAHHVHFDY
                     AFIKSELERLGKYFYKDRFCTVNLSRKMLPQNTKHNLGALIEYFQIPVKARHRALDDA
                     QATAEALIRFLKMPNAEEIFSKYAEQFDRRDKQLERLEHELFKLPNVRGVYMFKDTHD
                     LPLYIGKSNHIRSRILGHVREFDIPRKRRLMKYTDHFDFTPCSSELEALILESRLIKQ
                     HQPPYNIQQRARKGYLFIKVTPDPYPRVFQVAEKKADGGHYIGPFRSGKFLDHMLRKL
                     QRHYRLCPELMKERRSPKGFCFAHQLRQCSGACGEAISAEEYREQVEDAMAVLAEYIR
                     IESKENIDSFLKSRDARSPQLVEFRKALRRVRRHMDDNPDAFDEKFLLIQKEDRVGYF
                     IHNGLLSKVLHGEELENPNLLEALSTISTNGLIETPEILDERMTIQRFVRTHRHKLSI
                     IPIRQYDFAQ"
     gene            complement(4548..5081)
                     /locus_tag="PK754_06500"
     CDS             complement(4548..5081)
                     /locus_tag="PK754_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09188.1"
                     /translation="MPVNRKVFSLRFIFIFSVLLSGIWLLSGCDELMDDENDDSDCDP
                     KPLDCHETKYSHGYLNINLTINGKNNSVPIRIFRGDFDNNDLLLDTIVTVNTFSYRLP
                     TEEYYSVVARYIQNDGDTVLAVDADDIENDRESYCDGDCYELDNGSIDLKLRFPKETA
                     ESVYSTRYIVRSRQKIQ"
     gene            complement(5081..5644)
                     /locus_tag="PK754_06505"
     CDS             complement(5081..5644)
                     /locus_tag="PK754_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09189.1"
                     /translation="MGYPSGLRAQFDRGGAMSYENRAKHSAYFEMPFRLPGFGGKNYG
                     DLALGGTYRHNINGNQFLGVFGSFYGRPYGKAVLVKAGPRFYFQAKEYRYVIAGGLDK
                     KFWINNKLDLFIGAGIGVTFIDYRGTSSGIIDGHKLEKKEGWTPVVQAGFSHKFSRFV
                     FIRSGLQYMNMRTIPGLRFYAALGGQF"
     gene            complement(5683..>5979)
                     /locus_tag="PK754_06510"
     CDS             complement(5683..>5979)
                     /locus_tag="PK754_06510"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066424.1"
                     /note="catalyzes the formation of glycerone phosphate and
                     D-glyceraldehyde 3-phosphate from D-fructose
                     1,6-bisphosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HNB09190.1"
                     /translation="GKTHKKVYDELTTENAIDLTRYQVANCYMGRAGLINSGGESKGA
                     SDLAEAVKTAVINKRAGGMGLISGRKAFQKPMGDGVRLLNAIQDVYTTKEITIA"
     assembly_gap    5980..5989
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5990..6738)
                     /locus_tag="PK754_06515"
     CDS             complement(<5990..6738)
                     /locus_tag="PK754_06515"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HNB09191.1"
                     /translation="MLKTIQELLGAEADALLKYECKGIKKDQIHIPGPDFVDRIFIHS
                     DRHPQVLRSLQSLFGTGRLAGTGYLSILPVDQGIEHSAGASFAPNPIYFDPENIVKLA
                     VEGGCNAVASTYGVLGACARKYAHKIPFIVKINHNEFLSYPNKYDQIMFGSVKQAWDM
                     GAIAVGATIYFGSAESARQIQEVSEAFAYAHELGMATILWCYTRNNAFKTSEKDFHTS
                     ADLTGQANHLGVTIQADIIKQKLPTNNGGYEA"
     gene            6895..9801
                     /gene="uvrA"
                     /locus_tag="PK754_06520"
     CDS             6895..9801
                     /gene="uvrA"
                     /locus_tag="PK754_06520"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNB09192.1"
                     /translation="MKLNDDTQLTLDYEKKPTRSESDLFKETIIVRGARVHNLKNVDL
                     EIPRNKLTVITGLSGSGKSSLAFDTIFAEGQRRYVESLSAYARQFLNMMEKPDVDLVD
                     GLSPAISIEQKTASRNPRSTVGTVTEIYDYLRLLYAKVGVPYCYNCGREVKKQTRDQI
                     IEAILKMKKGARIQILAPVVRGRKGHYQELFEKLRKSGFVRVRIDGEIRELEEVDKVS
                     RYQIHNIEVVIDRLELPEKADQRLIDSVEQALEIGEGVLIVVSGEGKKNSDVTYSQHL
                     ACLHCAISYDEPFPKAFSFNSPFGACPACEGLGVIREIDETLIIPDRAKTVNEGAIAP
                     YDNPKADTWFYLQMKKILEMNGADFDTPIHKLPKKAYEMLMEGTGKTKHAFYYYSGNK
                     KKNYNGTYKGVKGIIRHFFSDTSSEQIREWAEGFMSEKVCPSCLGGRLKKEALSYRIG
                     DKNIHDAVHMSVQRCADFFDKYRIDPRIQKIGEPILKEINERLGFLLKVGLDYLTLDR
                     SARTLSGGESQRIRLATQVGSQLVGVLYILDEPSIGLHQRDNEKLIQALTDLRDLGNT
                     VIVVEHDRDTMEHADFLVDLGPGAGRLGGKIVAAGAPGDFARFDSVTAKYIRNEARIE
                     VPSVRREGNGKVLTLKNASGNNLKNVDLHIPLGKLVCVTGVSGSGKSSLINETFYRVL
                     ARHFYKAKDAPLPYKSIKGLDHIDKVIDIDQSPIGRTPRSNPATYTNLFSLIRDLYAG
                     LPEAKVRGYKVGRFSFNVTGGRCEECQGAGLKKIEMNFLPDVYVPCETCEGKRYNRET
                     LEIRYKGKSISEVLEMTVAEALDFFDAVPPIKRKIQTLHDVGLDYIHLGQQATTLSGG
                     EAQRVKLAEELSKSSTGKTLYILDEPTTGLHFEDIKMLMNVLGQLVDKGNTVVIIEHN
                     LDVIKCADWVIDIGPEGGDGGGNIVGEGTPEEIAKIKDSYTGKFLKQELRRFRN"
     gene            9888..10919
                     /gene="hprK"
                     /locus_tag="PK754_06525"
     CDS             9888..10919
                     /gene="hprK"
                     /locus_tag="PK754_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNB09193.1"
                     /translation="MEDILYRIEKDNTRVVEKMAVRRLYEDLKEKIHLELIVSEGMDI
                     RYFAQRDLHRPGLALAGFVKLFTHDKIQILGNTENYYLLSQDDSERHVALETIFEFEM
                     PCIIVTNGNKIMPEMSALCEKKKVPLMVTELSTTDTIHLISNYLDEMFAPEMTMHGSM
                     VDVYGIGMILMGKSGIGKSEIALDLVERGHRLVADDTIRISRTSGGILMARGHDTLKH
                     FLEVRGVGILDVREMFGIRAVRVQKRVEVVVDLQLWDGKEEYERLGLDDHTVEYLGVK
                     IPIVKLPIYPGKNITVIAEAIALNLLLKVYGYDAAKIFTDRLSEEIRNKNKNAKSYVT
                     NKFLDGDKE"
     gene            10935..>11596
                     /locus_tag="PK754_06530"
     CDS             10935..>11596
                     /locus_tag="PK754_06530"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNB09194.1"
                     /translation="MNENKVMEIKVGITILLATVLLVMSIVWLKGITFKPNTFEVEIT
                     FNNTAGLQIGDPVTVSGMKVGKVTDINLEADSVIVSVSLSNSVILKEDVRAVITSTDF
                     FGGKKIELVTGVADNMFKSKRFRGTREPDITELTSQLKEIATDVKGTLQRVDSVLIGV
                     NNFVGDKKMINSLKNAVYNLDSTTSRVRQIVERSDVRIDSTLSRLSSSTKIFKTMLSR
                     TDA"
BASE COUNT         3409 a         2367 c         2607 g         3203 t
ORIGIN      
        1 atgaaaattg ggatatcgaa gcgggtctaa ggtatgatta ttttgattca ccgagggact
       61 atcagtttgt tgatccacgt ttggcggcta aatatcgtat taatgaaact acgaacctca
      121 aagcgtcggc cggtcgttat catcagtacg tgcatcgcat accgagaact ttttttgcca
      181 gtatatggac taccaccgat caatatcaaa aagcatcaga ggcataccac tttataaccg
      241 gatttgaaaa aacctttgac gatgtttatc aagttgaagt agagacctat tataaaaaat
      301 ataatagtat ttatacgctt aaccgttttt tggtgacgga tatgaagccg tcttattatg
      361 atgaacaagg caatcctatt tttacgaata ccaacggtat ttttaatcac ggaaacggta
      421 attcgatcgg tcttgaattg atgcttagaa aagacgtcgg tgctttcact ggatgggtgg
      481 gatattcgct ggccagaacg gaatataatg tggaaggaaa aaatcacggt cagtcgttta
      541 caccgcgaca tgaccgtaca catacgatca atgcggttgg aaacatagac attcgtaacg
      601 gtatgagagt tatccggggt cttgagacta ccaaatcaaa cagtcggtgg actcttggtt
      661 ttaattttat ttattcgtcc gggcaaccta taacccgccc aggctcgctt tatgtcataa
      721 ccccaacgcc ggacaacggt aatcctgtta ttatcgaata tccggatgat ataaataatt
      781 accgtttgcc ctattatgca cgtttggacg tgagtttgac ctatgaaaaa cagtttaaat
      841 cgtggtctat cgctccgtat ttgcaaattt acaacatcgg aaaccgaaaa aatgtttggt
      901 ttatagacta tccgtatgaa ataaaagagg gcggcacgat aaaacccaaa gtaaaaacaa
      961 cctccatgtt tcccatgctt cccagcatcg gtatcaattt tacattttaa gaaggcatat
     1021 atatgaataa gtttaagttt ttattttata taccattatt actgattacg gcttgcggtg
     1081 agggtaaagt ttcaatagat aacatcaact ataaacctaa gatcgttatc aggggattga
     1141 tttttccggg gcaacccgtt tccggtataa gtataactcg caatctgcca atacgtgaac
     1201 aacaattgac attggatcaa ctcatcatcg cagatgccaa agtcgtaatc acaaacgtat
     1261 cgtccacggt tacagataca ctcgtatttg atccgattac ggcgacttat tatgatccgg
     1321 aacacaattt ttccgttcaa tataatacaa tctatagact aaatgttgca gctcagatag
     1381 acggtaagaa tctgaacgcg agctccgaaa cgaaagttcc gcagcagggt tttagtatca
     1441 ttgaagaaag ttcccgatta gatagtttgc actaccggga gagggatgaa aaagacagta
     1501 tccgcacatt tacagttcgt tttgagcgat ccgtcaatgc agaaacttat cttatgtcaa
     1561 tgaatgcctt ggatgcggat acttctacat acgtttattc gccggtcaac ccttattccg
     1621 atctaaaacc ttcggatgtt ttgaaagatt tggatgattt taaatattcc acccaatgga
     1681 gctcactttt tgaaaaccaa cccggtcaat cagaaatgag cgtattttgg tttttcctat
     1741 cgttttatgg gagatatcag atggtcgtgt atgcagcgga tgagaattat tcagatttta
     1801 tcaacaccta cgatcaggta caagatattg atggtaacta cgatgaaccg gtgtttcaca
     1861 ttgacggaga tgggatcgga gtgtttggtt cggctattgc tgatacggta tattttaaag
     1921 tattgaagcc ataagaagga tatataaaaa acatatacct aggattgtta cttggagcga
     1981 tttttatata acacataata tcctgcgccc aataaaccgg cagtatttcc taactttgcc
     2041 cgtactattt gaagttttgg cttaataggt ttcataactc ttttgatgac ttcattatga
     2101 atacggctta caaacgcgtc gccggcttcc gatagacctc cgccgatgat tattatatcg
     2161 gggttgagaa catgaactat gctcgacaac ccggctccga gataaaaaac catttgttct
     2221 acaatgttta ggcaaagcgg gtcgttcttt tgagcaaaat caaaaatagt cttcggggtg
     2281 atattttcta ccgaggagag cacggactta tcgccacgag caatagcaga taaagcctct
     2341 ttcacgatat atttaccgcc tgcatattgc tccaaacatc cttggttacc gcaaccacat
     2401 gggcggccgt gtgcttcgac gatcgtatga cccaattctg caccattgta tgaggagccg
     2461 tgaaatatat ttccgtttat gataattcca ccgccaacac ccgttcctaa cgtaatacct
     2521 acgaccaatt tatattgttt tcccgctcca agcatcgctt cgccgaatgc cataaaatta
     2581 gcatcattat cagcaaaaac aggaaggttc attttttgtg aaatatattc agcaatcggt
     2641 gcattttccc aatcggggag atgcggggtg ggtccaagcg aaatccctcg gtcgctgtca
     2701 atagttcccg ggcatccgac tccaattcct tcgacttcaa atgattcttt cctaccgaac
     2761 atcacacatt cataagcggc atccaccatg gtttgcagta catggtcgcg tccatgatcg
     2821 gcaaatacag gtattacatt ttgatatatg acctcgccgc ggtcggagac gatgccatat
     2881 tttaagttgg tgccacctaa gtccaaacct accgcatagc ggcgcggttt atcttcatgt
     2941 aacaatcggt cattgagcga aatcatattg ccggatagga ataatgctga gtttgtgccg
     3001 gtgagtgcgt acaaaacgtt gtatagtcat gcgttcatct aaaatttcgg gagtttcgat
     3061 caaaccgttt gttgatatcg tcgacagagc ttccagtagg ttggggtttt caagttcttc
     3121 gccgtgtaat accttagaca aaaggccgtt atgaatgaaa tagccgacac gatcttcctt
     3181 ttggattaac aaaaactttt catcaaaagc atccgggtta tcatccatat gtctgcgaac
     3241 gcgccgtagc gcttttctaa actcgaccaa ctgcggagag cgggcatcac gcgattttaa
     3301 aaaggaatcg atgttttctt tactttcgat acgaatgtat tcagcgagga cggccatggc
     3361 atcttcgact tgttcacggt attcttcggc ggagatggct tcaccacaag cgccgctgca
     3421 ctgacggagc tggtgcgcaa aacaaaatcc tttcggtgaa cgccgttcct tcattaattc
     3481 agggcataaa cgatagtgcc tttgcagttt gcgcaacata tgatcaagaa acttaccgga
     3541 gcgaaacggt cctatataat gacccccgtc cgcttttttt tctgctacct gaaaaactct
     3601 cggatatgga tccggcgtta ctttgatgaa taagtaccct tttcgggcac gttgctggat
     3661 attataaggc ggttgatgtt gttttataag acgggattct aatatgagtg cttcgagttc
     3721 ggaggaacat ggagtaaagt caaaatgatc ggtatatttc atcaaccgac gttttcgagg
     3781 aatgtcaaat tcacgtacat ggccgagaat acgtgagcgt atgtggttac ttttaccgat
     3841 atatagcggc aaatcatgtg tatctttgaa catatacacg ccgcgcacgt tggggagttt
     3901 gaataactcg tgttctaaac gttctagctg cttatcccgg cggtcaaatt gttcggcgta
     3961 ttttgaaaaa atctcttcgg cgttaggcat tttaagaaac cgtatcaaag cttctgctgt
     4021 tgcttgcgcg tcatctagag cccggtgcct tgctttgacc ggtatttgaa aatattcgat
     4081 caaagcgccg agattatgtt tggtattttg gggaagcatt tttcgagaga ggtttaccgt
     4141 acaaaaccga tctttgtaaa aatatttacc gaggcgttct aattccgact tgataaaggc
     4201 ataatcaaaa tgcacatggt gtgcgacaaa ataatgccct tccatcatcg cctcgacttt
     4261 atgggcgata tccgcaaata ccggtgcact tgaaacgtca cgatcggtta atccggtgag
     4321 tttggtaata aacggtggga tcggaatggg cggtttaact aaagaatgga acgtatccgt
     4381 aattttgaaa tcacgtacat tgacaatgcc gatctcaatc acatagccga tggagctttt
     4441 atcaccggtt gtttcgacat caacgacgga aaaacctgcc gaaagatcaa ataatgaaga
     4501 cggcataaca aagggaactc aggcgaacgt gtgttaaaac gttcaggtta ttggattttt
     4561 tgacgagagc gaactatata tcgtgttgaa taaacagatt cggccgtttc tttgggaaaa
     4621 cgcaacttaa gatcaatgga accgttatcc aattcataac aatctccgtc gcaatacgat
     4681 tcgcggtcat tttcgatatc atcggcgtcc acggcaagta cggtatcacc atcgttttgg
     4741 atatatcgag ctacgacaga atagtattct tctgttggca gtcggtagct aaacgtattt
     4801 acggtaacta tcgtatccag caaaagatcg ttattatcaa aatcacctcg aaagatacga
     4861 atcggaacgg aattgttttt tccgttaatg gtcagattaa tattgaggta gccatgcgaa
     4921 tatttagttt catggcaatc caaaggctta gggtcgcaat cactgtcatc gttttcgtca
     4981 tccatcaatt catcacatcc gctgagtaac caaataccac tcaataaaac ggaaaaaata
     5041 aaaataaaac gcagtgaaaa aacttttcgg ttaaccggca ttagaattgt cctcccaaag
     5101 ctgcatagaa acgaagaccg ggtattgtgc gcatattcat gtattgtaaa cccgatcgga
     5161 taaaaacaaa tctggaaaat ttatgagaaa aaccggcttg aaccacgggt gtccaacctt
     5221 cttttttttc taatttatgt ccgtcaataa taccagagct tgtaccccga taatcaataa
     5281 aagtcacgcc tataccggca ccaataaaaa ggtcaagctt attattaatc cagaattttt
     5341 tatccaaacc gccggcaata acgtagcgat attctttggc ttgaaaataa aaacgaggcc
     5401 cggcctttac taagacagct tttccgtaag ggcgaccata aaacgaaccg aaaacgccta
     5461 aaaattgatt accgttgata ttatgacggt aggttccgcc caaagcaaga tcgccgtagt
     5521 ttttaccgcc aaaaccaggt aaacggaaag gcatttcaaa atacgccgaa tgtttggcgc
     5581 ggttttcgta tgacatcgct ccgccacgat caaactgtgc gcggaggccg gatggatagc
     5641 ccaaacaaaa caaaaaagag aatagaataa tttttttcat catcaagcaa ttgttatttc
     5701 tttggtcgta tatacatctt ggatagcatt aagtaaacgt acaccatctc ccattggttt
     5761 ttggaatgct ttgcgcccgg aaataagacc cataccgccg gcccgtttat tgataacagc
     5821 tgtttttaca gcttccgcca aatcactggc gcctttggat tcgccacccg aattgataag
     5881 tcctgcacgg cccatataac aattggccac ttgataacgc gttaaatcaa tagcgttttc
     5941 cgttgtaagt tcatcataaa cttttttgtg cgttttgccn nnnnnnnnng cttcataacc
     6001 gccgttattg gtcggaagtt tttgtttgat aatatcggct tggattgtaa caccaagatg
     6061 attggcttgt cctgtaagat cagcggaagt atgaaaatct ttttcagaag ttttgaatgc
     6121 gttgttacgc gtatagcacc acaaaatagt agccattcca agttcatgtg cgtacgcaaa
     6181 agcttcgctg acttcctgta tttggcgtgc ggattcggca gaaccaaaat aaatcgtggc
     6241 accgacagcg atcgcaccca tgtcccaggc ctgcttgacg gaaccgaaca tgatctgatc
     6301 gtatttgttg ggataagata aaaattcgtt atgattgatt tttacgataa aagggatttt
     6361 atgcgcatac ttgcgtgcgc aagcgcccag tacaccatag gttgaagcga cggcgttgca
     6421 accaccttct acggcgagtt tgacgatatt ttcaggatca aaataaatcg ggttgggagc
     6481 aaaagaagcg ccggctgagt gctcaatgcc ttggtcaaca ggaaggatgg ataaataacc
     6541 cgttccggct aaacgtcctg tgccaaataa cgactgtaaa ctgcgcaata cctgaggatg
     6601 gcggtcgcta tggataaaaa tacgatctac aaaatccgga ccgggaatgt gaatctgatc
     6661 ttttttgatg cctttacatt catatttcaa aagtgcatcc gcctctgcgc ctaagagttc
     6721 ctgaatggtt ttaagcataa atctctcccg atattttaaa ttgaacaaat attattctta
     6781 ttaaaaacga agaaacttaa ccaaatttat aagcccattc aatgagaatt ttgtggcgaa
     6841 gtagtaaaaa actgccttat attgccgccc tgactaactc agctattgtg aaaaatgaaa
     6901 ctaaacgacg atactcagct cactttggat tacgaaaaaa aacctacccg atctgaatcg
     6961 gatctattta aagaaacgat catcgttcgc ggtgcgcgag tccacaatct taaaaatgtc
     7021 gatttggaga ttccgcgtaa caaacttacg gttattaccg gtttatcggg ttccggtaaa
     7081 tcctcgttag cgtttgatac gatttttgcc gaaggccagc gtcgatatgt cgaatctctt
     7141 tccgcgtatg cccgtcaatt tctcaatatg atggaaaaac cggatgtgga tctcgtagat
     7201 ggtctgagcc cggcgatcag catcgagcaa aaaacagcta gccgcaatcc acgttctacc
     7261 gtgggtacgg ttacggaaat ctatgattat ttgcgattgt tgtatgccaa agttggcgtg
     7321 ccgtattgtt ataattgcgg acgtgaagta aagaaacaaa cgcgcgatca aatcatcgaa
     7381 gcaattctca aaatgaaaaa aggtgcacgg attcagattt tagcaccggt cgtgcgaggc
     7441 cgtaaaggtc actatcagga actgtttgag aaattacgca aaagcggatt cgtacgcgtt
     7501 cgaatagacg gagaaatccg cgaattggaa gaagtagata aagtttcacg ttatcagata
     7561 cacaacatcg aagtcgtgat agatcggctt gagttaccgg aaaaagcgga tcaacgtctt
     7621 atagattctg tggaacaggc gctcgaaatc ggtgaaggtg tattgatagt tgtttccggt
     7681 gaaggcaaaa aaaatagcga tgtgacttac agtcaacatc tagcgtgtct gcactgtgcg
     7741 atcagttatg atgagccttt tcccaaggct ttttctttca atagcccgtt tggcgcttgt
     7801 ccggcgtgtg aaggtctcgg cgtaattcgt gaaatcgatg aaaccctcat tatacccgat
     7861 agagcaaaaa cagtcaatga aggtgccatc gctccgtatg ataatccgaa agccgatacg
     7921 tggttttacc ttcagatgaa aaaaatattg gaaatgaacg gtgctgattt tgatacgccg
     7981 attcataagc tgccaaaaaa agcgtacgaa atgttgatgg aaggtaccgg aaaaacgaaa
     8041 catgcgtttt attattattc aggcaataaa aagaaaaact acaacggaac gtacaaaggc
     8101 gtaaaaggta ttattcgtca cttttttagc gatacctcgt cggagcaaat tcgcgaatgg
     8161 gctgaaggtt ttatgagtga aaaagtatgt ccttcatgcc tcggtggccg tctcaaaaaa
     8221 gaagcgcttt cgtatcgcat cggcgataaa aatattcatg acgccgttca tatgtcggtt
     8281 cagcgatgcg ctgatttttt tgataaatac cgtattgatc cgcgcataca aaagatcggg
     8341 gaaccgatac tcaaagagat caatgaacgg cttggttttt tgctcaaagt cggtttggat
     8401 tatctgacgc ttgatcgcag tgcgcgtacg ctttccggcg gtgaatcgca gcgtattcgt
     8461 ttggctacac aagtcggttc acaacttgtt ggtgttttat atattctcga cgaacccagt
     8521 atcggtttac atcaacgcga taatgaaaaa ctgatacaag ctttgacgga tttacgcgat
     8581 cttggtaata cggttatcgt cgtcgagcat gatcgtgata cgatggagca cgccgatttt
     8641 ttggttgatc tcggaccggg ggcaggtcgg cttggcggaa aaatcgttgc ggccggtgcg
     8701 ccgggtgatt tcgcacgttt tgattccgtt acggcaaaat atattcgcaa tgaagcgcgt
     8761 atagaagtgc cgtcggtgcg acgcgagggc aatggcaaag ttctcacgtt gaaaaacgcg
     8821 agtggaaata atctgaaaaa cgtagatctg catattccac tcggaaaact tgtgtgtgtg
     8881 accggtgtta gcggatcggg caaaagttcg cttatcaacg aaacttttta tcgtgtgctg
     8941 gcacgtcatt tttataaagc taaagatgca ccgctgccat acaaatctat caaaggttta
     9001 gatcatattg acaaagtcat agatattgat cagtcgccga tcgggcgaac accgcgcagc
     9061 aatccggcaa catataccaa tctttttagt ttaatccgtg atttgtatgc cggtttgcca
     9121 gaagctaaag tgcgtggtta caaagtagga cgttttagtt ttaatgtgac aggcggacgg
     9181 tgcgaagagt gtcagggcgc cggattgaaa aaaatcgaaa tgaatttttt gcccgatgta
     9241 tatgtaccct gcgaaacatg tgaaggcaaa cgatacaatc gcgaaacttt ggaaatccgc
     9301 tataaaggga aaagcatatc cgaagttttg gaaatgacgg ttgcagaagc gctggatttt
     9361 tttgatgctg taccacccat caagcgtaag atacaaacac tgcacgacgt cggattggat
     9421 tatattcatc tcggtcagca agccacgaca ctttccggcg gagaagcgca gcgggttaag
     9481 ttggcagaag agctctccaa atccagcacc ggaaaaacac tttatattct tgatgaaccg
     9541 acgacggggc ttcattttga agatatcaaa atgttgatga atgtgctcgg ccagcttgtc
     9601 gataaaggca ataccgtggt gatcatcgag cataacctcg atgtgatcaa atgtgcggat
     9661 tgggtgatcg atataggacc ggaaggcggc gacggcggag gtaatatcgt cggcgaagga
     9721 acaccggagg aaattgccaa aatcaaagat tcttataccg gcaagttttt gaagcaggaa
     9781 ttgcggagat ttaggaatta aaaattgatt ggttggtata taatgtttag attcgcccgt
     9841 acttagaaaa aagtatattt taataaagca taagtcggaa tacgtccttg gaagatattc
     9901 tttaccgaat cgaaaaagac aatacgcgcg tcgttgaaaa aatggcggtt cggcgtttgt
     9961 acgaagattt gaaagaaaaa attcatctgg agcttatcgt ttccgaaggt atggatattc
    10021 gatattttgc ccagcgcgat ttgcaccgac cgggattagc gttagcgggg tttgttaaac
    10081 tttttacgca tgacaaaatt caaatcttag gcaacacgga aaattattac ctcctgagcc
    10141 aggacgacag cgaacggcat gtcgcgcttg aaacgatttt tgaatttgaa atgccgtgta
    10201 tcattgttac taacggcaac aaaatcatgc cggaaatgag cgctctctgt gagaagaaaa
    10261 aagtgccgct catggtgaca gagctttcga cgacggatac gatccatctg atcagcaact
    10321 atctcgacga gatgttcgcc ccggaaatga cgatgcatgg gtctatggtg gatgtttacg
    10381 gcatcggtat gattctcatg ggcaaaagcg gtatcggcaa aagcgaaata gcgctagatc
    10441 tcgtcgaacg cggccaccgt cttgttgcgg atgatacgat tcgcatcagt cgtacttcgg
    10501 gcggtattct gatggctcgc ggacatgata cgctcaagca ttttcttgaa gtgcgcggtg
    10561 tcggcattct tgatgtgcgt gaaatgttcg gtatccgtgc cgtgcgtgta caaaaacgtg
    10621 tcgaggtcgt ggttgattta caactttggg acggcaaaga agaatacgaa cggcttggct
    10681 tggatgatca taccgtcgaa tatctcggcg taaaaatacc gatcgtcaaa ctgccgattt
    10741 atccgggaaa aaatattacg gttattgcgg aggccattgc actcaacctg cttctcaaag
    10801 tttacggtta cgatgcggct aaaattttta cagatcggtt gtctgaagaa ataagaaata
    10861 aaaataaaaa cgctaagtcc tacgttacca ataaattttt agatggcgat aaagaataaa
    10921 aaaagaggga aagtttgaac gaaaataaag tcatggagat caaggtcggt attacgatac
    10981 tattagcgac cgttcttttg gttatgagca tagtgtggct caaagggatt acttttaagc
    11041 caaacacgtt tgaagtcgaa ataactttca ataatacagc cggattgcag atcggtgatc
    11101 ccgttacagt cagcggtatg aaagtgggaa aagtaacgga tattaatctg gaagccgatt
    11161 ccgtaattgt gtcggtcagt ttatccaaca gcgtcattct caaagaagat gtacgtgccg
    11221 ttattaccag cacagatttt tttggcggaa aaaagatcga gttagtcacc ggcgtagcgg
    11281 ataatatgtt caaatccaaa cgatttcgcg gtacgcgtga acccgatatc acggagctga
    11341 caagtcagct caaagaaatc gctaccgatg tcaaaggtac gctgcagcgc gtggacagcg
    11401 tattgatcgg tgtaaataac tttgtcggcg ataaaaaaat gatcaattcg ctgaaaaacg
    11461 cggtatataa tcttgattcg actacatcgc gcgtacgtca aatcgttgag cgcagtgatg
    11521 tgcgaattga ttcgacgctt tcgcggctct catcctctac caaaattttc aaaaccatgt
    11581 tgagtcgtac ggatgc
//
LOCUS       DAOQNQ010000075        11585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_1982_length_11585_cov_4.928101, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000075 DAOQNQ010000000
VERSION     DAOQNQ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11585
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1982_length_11585_cov_4.928101"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="PK754_06535"
     CDS             complement(<1..1160)
                     /locus_tag="PK754_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNB09195.1"
                     /translation="MPFDKAFESVNRLVQDFRAQEAYYLDAKYQEQEARKDFIDKFWI
                     ALGWDVNHEIQKNPYEQEVRIENAVRTGASQRRADYGFYLSPNFRDPKFFVEAKKPSR
                     SIANPDDYFQVNRYGWSQQTPVAVLTDFEELHIIDCRFKPHIGTALDRKIKAFRYTDY
                     TDAEKFAEIYWLFSREAVATGSLEKYAAALPRPKGKAAQKGLFKAGVQPIDEAFLEEL
                     DEYRVILAKAFKKSNPDLEGEALTEAVQRTIDRLVFIRFLEDKSIEDYEVRHYGDSGH
                     AWKKFLAHCQRLEPKYNGLVFKPHPVIDGRAFHAPDEEDFKAICAKLSDPHAVYDFDQ
                     IPIAILGSIYERFLGKVVHTTAKRADVKVKPEVRKAGGVYYTPEYIVRYIVQ"
     gene            1416..2864
                     /gene="sufB"
                     /locus_tag="PK754_06540"
     CDS             1416..2864
                     /gene="sufB"
                     /locus_tag="PK754_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNB09196.1"
                     /translation="MNTETSELQQLADREYKYGFVTDIEADAAPKGLNEDIIRFISKK
                     KNEPEWLLEWRLKAYKQWLKMCEEGEPTWQNVHVAKVDYQDIIYYSAPKQKKKSADEV
                     DPELLKTYEKLGIPLNERLALAGVAVDAVFDSVSVATTFKTKLKELGIIFCSFSEAVQ
                     EHPELIKQYIGSVVPPTDNFYSALNSAVFTDGSFCYIPKGVRCPMELSTYFRINAAST
                     GQFERTLIVADEGSYVSYLEGCTAPMRDENQLHAAVVEIYAHTNAQVKYSTIQNWYPG
                     DKDGKGGIYNFVTKRGLCAGDNSKISWTQVETGSAITWKYPSCILKGDNSVGEFYSVA
                     LTAKRQQADTGTKMIHLGKNTRSTIVSKGISAGHGQNTYRGLVRVGKNAENARNFSQC
                     DSMLLGDQCGAHTFPYIEVKNNTAQVEHEASTSKIGEDQIFYCNQRGISTEDAVSMIV
                     NGFCKEVFKELPMEFAVEAQKLLGVSLEGSVG"
     gene            2867..3904
                     /locus_tag="PK754_06545"
     CDS             2867..3904
                     /locus_tag="PK754_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09197.1"
                     /translation="MSDFFNIYFDWFLGYLFLVLPGIVVFTLMIVKLFKIVTKRNRWI
                     TFSAMALLIHTIFFVYYDHQLSRMGEVTRIISADDVLMVEGVLTNTGYRSSRGSASGF
                     REDPATTTQAQKIFVFDKKSGNILQRIEDYQVAHYAERRAFLVPTIATKSYAIFDTRV
                     SQFDRFFISSDLRSDMPELANRIDKITYDRSWMLKPDMSGSGWDEAVPYFELTLSDGS
                     GLYYDVLTGETHPRDKKQTSWTDEKRPALFTEYADFTIDSSSTGVVRALKKRASENGF
                     VLFAVSADGQKKWTVTADQIHPEIKDDRFFFSENPGSYLVEDGKLYFFTYRFAVALDT
                     ENGNILWQQSI"
     gene            3944..4702
                     /gene="sufC"
                     /locus_tag="PK754_06550"
     CDS             3944..4702
                     /gene="sufC"
                     /locus_tag="PK754_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018474275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNB09198.1"
                     /translation="MLEIKNLHATIDGNPILKGINLSVKAGEVHAIMGPNGSGKSTLA
                     HILAGKEDYEITQGEIIFNGKSLTELDPEDRAAEGVFLAFQYPVEIPGVNNTYFLKAA
                     LNAIRKYRGEEPLDAMDFLSLVKQKMKLVEMDEDLLKRPVNEGFSGGEKKRNEIFQLA
                     VLNPKLAILDETDSGLDIDALKIVSNGVNKFRSGDNATIVVTHYQRLLNYIVPDFVHV
                     LMDGRIVKSGGKELALTLEDRGYDWIKEHDGVVA"
     gene            4702..6024
                     /gene="sufD"
                     /locus_tag="PK754_06555"
     CDS             4702..6024
                     /gene="sufD"
                     /locus_tag="PK754_06555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNB09199.1"
                     /translation="MINQTEAKNWFQNQFARFESALNGQKSGVTHALRRDAMARFQAS
                     EFPTMRHEEWKYTNLESLFTHGLHVASPTDSASHALDIRNDLTLDTEAYTVVFVNGYY
                     DAKRSTINAPEKGLQIRLLSQCSDEEKAGILRALAAETDESVFNTMHMALVSDGVVID
                     VAGGAVIGKPVHIQYFTVAEESAPMTQPLTLIRVEKNAQCTVIEDYKTVGENPFLTNA
                     RTEIIVAENAVVDHARIQNESVKGYHISLTRLRQARSSNFMTTHITFGAALSRNLTIA
                     HLDGEGIESTLNGLYMPKGRQHTDNRTIIHHAKPHCNSHELYKGIMDDHATAVFNGKI
                     FVHPDAQKTDAKQSNNNLLLSKDATINTKPQLEIFADDVKCTHGATIGRLEEKGMFYL
                     RSRGIGYDEARSIMTLAFAEDIVSRIRHTALRKRLDTMLHERLNMKFE"
     gene            6025..7275
                     /locus_tag="PK754_06560"
     CDS             6025..7275
                     /locus_tag="PK754_06560"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007145503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNB09200.1"
                     /translation="MNSTEQLKPFSDAEVAAMRRDFPILSEHVHGKPLVYLDNGATGQ
                     KPKQVIDAIARYYSRENSNIHRGVHYLSAQATIAYENARKKIQKLIHAPSDNEIIFLR
                     GTTEAINLVAQSYGRTYIQRDDEVLISAMEHHANIVPWQMLCEQTGAKLRVIPMNERG
                     ELRMDAYQTMLTEKTKIVAIVHVSNSLGTINPVKEMIALAHAKNIPVIVDGAQAVQHM
                     PVDVKDLDCDFYAFSGHKMFGPTGVGILYGKKKWLDAMPPYQGGGDMIRSVTFEKTTY
                     NDVPHKFEAGTPHIAGGIALGDTVDYLQGIGLERIAAYEKVLLDYATEKLSAVSGLHI
                     IGTAHNKAGVISFILDGVHPHDVGTMLDQEGIAIRTGHHCTQPVMQFFKVPATSRVSL
                     AFYNTHDEIDTLVKAIHKTMEMFR"
     gene            7272..7727
                     /locus_tag="PK754_06565"
     CDS             7272..7727
                     /locus_tag="PK754_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014148483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HNB09201.1"
                     /translation="MMSDLRELYQEVILDHNKNPRNFRVMENADIVKEGFNPLCGDHL
                     HLYLKLDGDVIQDVSFEGNGCAISKSSASLMTTVLKGKTKSDAEILFKRFHDLVTADM
                     STTVDEESLGKLAVFAGVREFPARVKCASLAWHTMHAAIANDTTIVSTE"
     gene            7731..8060
                     /locus_tag="PK754_06570"
     CDS             7731..8060
                     /locus_tag="PK754_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HNB09202.1"
                     /translation="MIEETKLGPTGMEIQVIEAIRTCYDPEIPVNIYDLGLVYEVKVD
                     AAGVASILMTLTSPSCPAAESLPPEVESKARAVPGITDVKLELTWEPPWDPTRMTEAA
                     RLELGMM"
     gene            8076..8543
                     /locus_tag="PK754_06575"
     CDS             8076..8543
                     /locus_tag="PK754_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNB09203.1"
                     /translation="MKFSTQEEYGLRCLLQIARSTNKNGMTIPQISQAEGLSEANVAK
                     MLRILRMGGIIESARGHEGGYRLARTADQIRIGDVLTVLGGRLFDHEFCEKHAGEENI
                     CSHGTDCTLRHVWQTVQTAVDDVLMRLTLQDLVHPAQKPITEIKIPSTLVSTT"
     gene            8557..9267
                     /locus_tag="PK754_06580"
     CDS             8557..9267
                     /locus_tag="PK754_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNB09204.1"
                     /translation="MPTVFHKAESRGFADHGWLRSYHTFSFAGYHDPKRMNFGLLRVL
                     NDDTVRPGSGFGEHPHDNMEIISIPLEGTLAHADSTGSEKAIRTGDVQIMSAGSGLTH
                     SEYNHSASAPVNFLQLWIYPKRRNIEPRYDQKTFDLASRKNQWVTVVAPDENAESLWI
                     NQDAVLSLAVVESGEISYTPRFKENGIYIFMIDGNAQYESQLLGRRDAIGIHPAGDCN
                     LIIRQPSHILLIEVPMNF"
     gene            complement(9264..10427)
                     /gene="nhaA"
                     /locus_tag="PK754_06585"
     CDS             complement(9264..10427)
                     /gene="nhaA"
                     /locus_tag="PK754_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNB09205.1"
                     /translation="MKKILNPIQEFIHYEQSSGIILIFCTIVSLALANTPLNEPIYQF
                     WHTRIGFATENIDLHKTILHWVNDGLMAVFFLLVGLEIKREIVSGELSSLRRASLPLF
                     GAVGGMLVPALIYVSFNQGTIYENGWGVPMATDIAFALAILGLLGSRIPVSIKVFLTA
                     LAIIDDLGAIIVIALFYSSTLFLNYKAAALVTVLVLILINRLGIRSLAIYLGLGILLW
                     YFVLKSGIHATIAGVILAMTIPYSKDETSSPLLALEHALHKPVQFFIMPIFALANTLI
                     VMDASVWVALFSTLSIGIFTGLFFGKVCGILIFCWIGTKLNLAALPDGVTWHHLIGTG
                     FLAGIGFTMSIFIAVLAFENQEGIQTTAKAAILAASIVSGLTGFMILRRTIKT"
     gene            complement(10448..11437)
                     /locus_tag="PK754_06590"
     CDS             complement(10448..11437)
                     /locus_tag="PK754_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNB09206.1"
                     /translation="MSDLTVTPHSDAPLNDAADLYRQISAALDTTVIGQSDIKREMII
                     ALLSGGHILMEGVPGIAKTLLAKTLAKALSCEFKRIQFTPDLMPSDVIGTNVFDPKSN
                     QFNVKRGPIFTHIALIDEINRAPAKTQSALLEVMEERQITIDGTRYLLPEPFMVMATQ
                     NPIEYEGTYPLPEAQLDRFMFKLIVPYPSASEEEQVLRAYHGGFDAHQIDQIKIEKPC
                     TPEMIARARHLIRQIVVDDGILKYITEIVRKTRETSGIMIGASPRATINLLLAAKTQA
                     ALANRTFVIPDDVKDLAPAVLRHRLVLQPETEMEGLRPDDMIRDVLNTVPVPR"
BASE COUNT         3151 a         2622 c         2909 g         2903 t
ORIGIN      
        1 ttttgtacga tgtagcgcac tatatactcc ggggtgtagt acacgccgcc ggctttgcgc
       61 acttcaggtt ttactttgac gtcggcgcgt ttggcggtcg tgtggacgac tttgccgaga
      121 aagcgttcgt agatactacc gagaatagcg atagggatct gatcaaagtc gtacacggca
      181 tggggatcgg aaagtttggc gcaaatcgcc ttgaagtctt cttcatccgg tgcatgaaac
      241 gcacggccgt caattacggg atgcggtttg aatacaaggc cgttgtattt gggctccagc
      301 cgttgacagt gggcgagaaa ctttttccat gcatgaccgc tgtcgccgta gtgtcgcact
      361 tcatagtctt cgatggattt gtcttccaga aatcgtatga acaccagacg gtctatggtc
      421 cgttgcacgg cttcggtgag cgcttcgcct tcaagatcgg gattggattt tttgaaggct
      481 ttggccaaga tgacgcggta ttcgtccaat tcctcaagaa aggcttcgtc aataggctgt
      541 acacccgctt tgaaaagacc tttttgtgcg gctttgcctt tggggcgggg tagtgcggca
      601 gcgtactttt ccagtgatcc ggtggcgacg gcctcgcggc taaataacca gtagatttcg
      661 gcaaattttt cggcatcggt atagtcggta taacgaaagg ctttgatctt acgatccagt
      721 gccgtgccga tatgcggctt aaatcggcag tcaatgatat gcaattcttc aaaatccgtg
      781 agtacggcga cgggcgtttg ctggctccat ccgtagcgat tgacttgaaa ataatcatcg
      841 ggattggcga tgctgcgtga gggttttttg gcctctacga aaaacttggg gtcgcggaaa
      901 ttgggggaga gataaaaacc gtaatcggcg cgccgctgcg aagcaccggt gcgaacggca
      961 ttttcgatgc gcacttcctg ttcgtacgga tttttttgaa tctcatgatt gacatcccac
     1021 ccgagagcga tccaaaattt gtcaatgaaa tccttacgcg cttcctgctc ctgatatttg
     1081 gcgtcgagat aatacgcttc ttgtgcacga aaatcctgca cgagacggtt tacggattca
     1141 aacgctttat cgaaaggcat gagaaaatgt ccccaagtaa gagtaagaaa agatgaataa
     1201 ccatctgggc aatctcgtta aacgaatgaa tttttgcaag aaatagaatt ataggcaata
     1261 cagaaaattt gtagtgttgc tgttaccaaa aaaaacacat ccaattagag tcacatttgc
     1321 cacttttgat acttgacaaa aaaatcaatt tatacttgac agtatagtac agattgttta
     1381 atctttcgcg tgttagagtc aacaatagga gttttatgaa taccgaaacc agtgaactgc
     1441 aacaactggc tgaccgcgaa tacaagtacg gctttgtaac ggatatcgaa gccgatgccg
     1501 cccccaaagg acttaacgaa gacattatac gcttcatttc caaaaagaaa aatgaaccgg
     1561 aatggctttt ggagtggcga ctcaaagcgt acaaacaatg gctcaaaatg tgcgaagaag
     1621 gcgagcctac atggcaaaat gtgcatgtcg ccaaagtgga ctatcaggat atcatctact
     1681 atagcgcgcc gaaacaaaag aaaaaaagtg ctgacgaagt ggatccggaa ttgctgaaaa
     1741 cgtacgagaa gctcggtatt ccgctcaatg aacgccttgc cctcgcggga gtggctgtgg
     1801 atgccgtatt tgacagcgta tccgtcgcga cgacattcaa aaccaaactc aaagaactcg
     1861 gtatcatttt ttgttcattc tctgaagctg tgcaagaaca tcccgaattg atcaagcaat
     1921 acatcggttc ggtcgtaccg ccgacggata atttttactc tgcgctcaat tccgcggtat
     1981 ttaccgacgg ttcgttttgt tatatcccca aaggcgtacg gtgcccgatg gaactttcga
     2041 cgtatttccg catcaacgcc gccagtacgg gccagtttga acgcacgctc atcgtggcgg
     2101 acgagggcag ctacgtcagt tatctcgaag gatgtaccgc accgatgcgc gatgaaaacc
     2161 aattgcatgc tgccgtcgtc gaaatctatg cgcataccaa tgcacaggtc aaatattcaa
     2221 cgatacaaaa ttggtacccg ggcgacaagg acggtaaagg cgggatttat aattttgtga
     2281 cgaaacgcgg tttgtgcgcg ggtgacaatt ctaaaatctc ctggacgcag gtagaaaccg
     2341 gctcggccat cacatggaaa tatccgagtt gcattttgaa aggtgataat tcggttggag
     2401 aattttattc cgtcgcgctc acagccaaac gtcagcaagc cgataccggt acgaagatga
     2461 ttcacctcgg caaaaatacg cgcagcacga tcgtttccaa aggtatctcc gcaggccacg
     2521 gccagaatac ctatcgcggc ttggtgcgcg tgggcaaaaa cgcggagaat gcgcgtaatt
     2581 tttcccaatg cgattcgatg ttgctcggtg atcagtgcgg cgcgcatact ttcccgtata
     2641 tcgaagtgaa gaacaatacc gctcaggtag aacatgaagc atccacgtcg aagatcggcg
     2701 aagaccagat tttttattgc aatcagcgcg gtatttctac cgaagacgcc gtgtctatga
     2761 tcgtcaacgg attttgtaaa gaagttttca aagaactgcc gatggagttt gccgtcgaag
     2821 cgcagaaact gctcggcgta agcctcgaag gcagcgtagg ttgaggatga gcgatttttt
     2881 caacatatac tttgattggt ttctgggtta tctctttttg gtattacccg gcatcgtcgt
     2941 gtttacactt atgatcgtca agctttttaa gatcgtgacg aaacgcaacc gctggattac
     3001 tttttctgcg atggcattac tgatacatac gatttttttt gtgtattatg accatcagct
     3061 ctcgcgtatg ggtgaagtga cgcgcatcat atcggcggat gatgtgttga tggtggaagg
     3121 cgtacttacc aataccggat accggagcag ccgcggcagc gcgtccggat ttcgcgaaga
     3181 ccccgcgacg accacacagg cgcagaaaat cttcgtgttt gataaaaaaa gtggaaacat
     3241 tctgcagcgc attgaggatt atcaggtggc gcattatgcc gaacggcgcg catttttagt
     3301 accgaccatc gccactaaat cgtacgcaat ttttgatacc cgtgtatccc aatttgatcg
     3361 tttttttata tcgagtgatc tgcgttccga tatgcccgag ttggcaaacc gcatagataa
     3421 gatcacatac gaccgttcat ggatgctcaa gcccgatatg tcaggctccg ggtgggacga
     3481 agccgtacct tattttgaac tcaccctgtc cgatgggtcc gggctttatt atgatgtgct
     3541 cacgggcgaa acgcatcccc gcgataaaaa acaaacctcg tggaccgatg aaaaacgacc
     3601 tgctctgttt acggagtatg ccgattttac gattgattcg tcctcaaccg gagtcgtacg
     3661 tgcgctcaaa aaacgcgctt ccgaaaacgg ctttgtatta tttgctgtga gcgcggacgg
     3721 gcaaaaaaaa tggaccgtaa cagccgatca gatccatccg gaaatcaaag acgatcggtt
     3781 ttttttcagc gaaaacccgg gttcgtatct ggttgaagac ggtaaactgt atttttttac
     3841 ataccgcttt gccgttgcgc tggatacgga aaacggaaac atcctttggc aacaaagtat
     3901 ttagatttat tcatataatc atttatagca gagaaaagaa gacatgctcg aaatcaaaaa
     3961 tctacacgca accattgacg gtaacccgat tctcaaagga atcaatcttt ccgtcaaagc
     4021 gggcgaagtg catgcgatca tgggacccaa cggttccggc aaaagcactt tggctcacat
     4081 tttagccggt aaagaggatt acgaaatcac gcaaggcgag atcattttta acggaaaaag
     4141 cctcacggaa cttgatcccg aagatcgcgc ggcagaaggt gtatttctcg cgtttcagta
     4201 tccggttgaa attcccggcg tcaataatac gtatttcctc aaagcggcgc tcaatgccat
     4261 tcgcaaatac cgcggcgaag agcctttgga cgcgatggat tttttgagtc ttgtaaaaca
     4321 aaaaatgaag cttgtcgaaa tggatgaaga cctgctcaag cgccccgtga atgaaggttt
     4381 ttccggcggt gagaaaaaac gtaatgagat atttcaactc gccgtactca atccgaaatt
     4441 agcgattctg gatgaaaccg attcaggtct cgacattgac gcactcaaaa tcgtttcaaa
     4501 cggcgtgaat aaatttcgct ccggtgataa tgcgaccatc gtcgtgacgc actatcagcg
     4561 tttactgaac tacatcgtgc cggattttgt gcacgtgctg atggatggac gtatcgtcaa
     4621 gtccggtggc aaagagctgg cgctcaccct cgaagatcgc ggttatgact ggatcaaaga
     4681 acatgacgga gtggtagcct gatgatcaac caaaccgaag ctaagaactg gtttcaaaat
     4741 cagtttgcac gttttgaatc cgcattaaac ggccaaaaat caggcgttac ccatgcgctg
     4801 cggcgtgatg cgatggcacg ctttcaggcg tctgaatttc ccacgatgcg tcatgaagaa
     4861 tggaaatata cgaatctgga atccctcttt acccacggac tgcacgtcgc ttcaccgacg
     4921 gatagcgcgt cgcatgcgct tgacatacgg aatgacttga cactggatac agaggcgtac
     4981 acggttgttt ttgtgaatgg atattacgat gccaaacgct cgacgataaa cgcaccggaa
     5041 aaagggttac aaattcgatt gctgtcgcaa tgcagcgatg aagaaaaggc cggcattttg
     5101 cgtgcgctgg cggcggaaac ggatgaatca gtgtttaata ccatgcacat ggcgctggta
     5161 tccgatggtg tggtgataga tgtggccggt ggtgcagtga tcggtaaacc ggttcatatc
     5221 caatacttca ccgttgccga agaatcggct ccgatgacgc agccattgac gctcatacgt
     5281 gtggaaaaaa acgcccaatg tacggtgatc gaagattaca aaaccgtcgg ggagaacccg
     5341 tttctcacca atgcgcggac tgaaatcatc gtagccgaaa atgccgtagt ggatcatgcg
     5401 cgcattcaaa atgaatccgt caaaggatac catatcagcc tgacacgcct gcggcaagcg
     5461 cgcagcagta attttatgac gacccatatt acgttcggcg cggcgttgtc ccgcaatctg
     5521 acgatcgcgc atttggatgg cgaaggcatc gaatctacgc ttaacggatt atacatgccc
     5581 aaaggtcgtc agcataccga taaccgcact atcattcatc atgccaagcc gcactgtaac
     5641 agccacgaat tgtacaaagg catcatggat gaccatgcga cggcggtttt caacggcaaa
     5701 atatttgttc atcccgatgc gcaaaaaacg gatgccaaac aatcgaataa taatttgctc
     5761 ttatccaaag acgcgacgat caataccaag cctcaacttg agatatttgc cgacgatgtg
     5821 aagtgtacgc acggcgcgac gatcgggaga ctggaagaaa aagggatgtt ttatcttcgc
     5881 tcgcgcggta tcggctatga cgaagcgcgc agcatcatga ctttggcgtt tgctgaagat
     5941 atcgtgtcgc gcattcgtca tacggcactg cgtaaacgcc tggatacgat gttgcacgaa
     6001 cggcttaaca tgaagtttga atagatgaac agtaccgaac aattaaaacc attctccgat
     6061 gcggaggtgg ccgctatgcg acgggatttt cccatattga gcgaacacgt tcacggaaag
     6121 ccgctggttt atttagataa cggtgcaacc ggacaaaaac cgaagcaagt gattgatgcc
     6181 attgcgcgtt attatagccg tgaaaattcc aatattcatc gcggcgtgca ttatttgagt
     6241 gcgcaggcaa cgatcgcata cgaaaacgcg cggaagaaaa ttcaaaaatt aatccatgcg
     6301 ccctccgaca atgagattat atttttacgg ggcacgaccg aagccatcaa tctggtagcg
     6361 caatcctacg ggcgaacgta tatccaacgt gatgatgaag tgctcatttc ggcgatggaa
     6421 catcatgcca atatcgtacc ctggcagatg ctctgcgagc agaccggtgc caaactgcgt
     6481 gtcattccga tgaatgaacg tggcgaactg cgcatggacg cgtatcaaac gatgctcacc
     6541 gaaaaaacga aaatcgttgc gatcgtgcat gtgtccaatt cgctcggtac aatcaatcct
     6601 gtcaaagaaa tgatagcact tgcacatgcc aaaaatattc ccgtgatcgt agatggcgcc
     6661 caagcggtgc aacacatgcc cgtggatgtg aaagatttgg attgtgattt ttatgcgttt
     6721 tcaggtcata aaatgttcgg tccgaccggc gtgggtattc tctacggaaa aaagaagtgg
     6781 ctggacgcca tgccgccgta tcaaggcgga ggggatatga ttcgttcggt gacgtttgaa
     6841 aaaacaacgt acaacgacgt gccgcacaaa tttgaagcag gcacgccgca tatcgcaggt
     6901 ggtattgctt taggtgatac cgttgattat ttgcaaggta tcggtttgga gcgtatcgca
     6961 gcgtatgaaa aagtattgct ggactatgca acggaaaaac tgagcgccgt ttcgggtttg
     7021 catattatcg gtacggcaca caataaagcc ggcgtgattt cgtttatatt ggacggcgta
     7081 catccgcacg acgtaggtac gatgctggat caggaaggaa tagccatacg caccggacat
     7141 cattgtacgc agccggtaat gcagtttttt aaagtacctg cgacgtcgcg cgtttcgctg
     7201 gcattttata atacgcacga tgaaatagat acgctggtga aagccattca caaaacaatg
     7261 gagatgtttc gctgatgtcg gatttgcgtg aattgtatca ggaagtgatt cttgatcata
     7321 ataagaaccc ccgaaatttt cgcgttatgg aaaatgcgga tattgtcaaa gaaggtttta
     7381 atccgttgtg tggtgaccat ttgcatcttt atctcaagct ggatggtgat gtgatccaag
     7441 acgtaagctt cgagggaaat ggctgtgcta tttccaaatc atccgcatcc ctgatgacca
     7501 ctgtgctcaa aggcaaaacc aagtcggatg ccgaaatttt atttaaacgg tttcatgatt
     7561 tggtaacagc cgatatgagt acgaccgtgg atgaagaatc gttgggtaag ttagccgtat
     7621 ttgccggcgt acgcgaattt cccgcccgcg tgaagtgtgc gagtttggcc tggcacacga
     7681 tgcatgctgc gattgccaat gatacgacca tcgtttctac ggagtgaacc atgatcgaag
     7741 aaaccaaact cggcccgacc ggcatggaaa tacaagtgat cgaagcgatt cgtacgtgtt
     7801 atgatcccga gataccggtg aatatttatg atctcggttt agtgtacgaa gtgaaagtgg
     7861 atgcggccgg agtggcgtct attctgatga cgctgacttc gccgagttgc cctgcggcag
     7921 aaagtttgcc tccggaggtc gaatccaaag cacgcgccgt gcccggtatc acggatgtaa
     7981 aattggaatt gacgtgggaa ccgccctggg acccgacccg catgaccgaa gcagcacgcc
     8041 tcgaactggg tatgatgtaa cgatacggaa aaaatatgaa gttcagtaca caagaagaat
     8101 acggattgcg atgccttttg cagatcgccc gcagtacgaa taaaaacggt atgacgattc
     8161 cgcagatcag tcaggcggaa ggtctttcgg aagccaacgt tgccaaaatg ttacgtatcc
     8221 tgcgcatggg cggtattatc gaaagtgcgc ggggtcatga aggcggctat cgtttagcgc
     8281 gaacggccga tcagattcgt atcggtgacg tgcttacggt attgggtggg cgtttgtttg
     8341 atcatgagtt ctgtgaaaaa catgcagggg aagaaaatat ttgttcgcac ggtacggact
     8401 gcacgctccg ccatgtttgg caaaccgtac aaacggcggt ggacgatgtg ttgatgcgac
     8461 tgacccttca agatcttgtg catcctgcac aaaaaccgat tacggaaatt aaaatacctt
     8521 ccacgctcgt atctaccact taaataagta ttccttatgc cgacggtgtt tcacaaagca
     8581 gaatcgcgcg ggtttgccga tcacggctgg ctccgttcgt atcatacatt tagttttgca
     8641 ggttatcatg acccgaagcg gatgaatttt ggcttgctgc gtgtactcaa tgatgacacg
     8701 gtgcgtccgg gatcgggatt cggtgagcac ccgcacgata atatggaaat tatttcgatc
     8761 ccgctggagg gtacgctcgc gcatgccgac agtaccggca gcgaaaaagc tattcgtacg
     8821 ggcgatgtac aaatcatgtc cgccggttca ggtttgacgc attcggaata caatcacagc
     8881 gccagtgcgc ctgttaattt ccttcaattg tggatttacc ccaaacgcag aaatatcgag
     8941 ccgcgctatg atcaaaaaac gtttgacctt gcttcacgaa aaaatcaatg ggttactgtc
     9001 gtggcgccgg acgagaatgc ggaatcgctc tggatcaatc aggatgctgt attatcgtta
     9061 gccgtggttg aaagcggcga aatttcatac acgccgcgtt tcaaagaaaa cggtatttat
     9121 atttttatga ttgacggcaa tgcgcaatat gagagtcaac ttcttggtcg tcgtgatgcg
     9181 atcgggatac acccggccgg cgattgtaat ttaataatac ggcaaccgtc gcatatattg
     9241 cttatcgaag tgccgatgaa tttttaggtt tttattgttc ggcgcagtat cataaaaccg
     9301 gtcagccctg aaacgataga tgccgccaaa atagccgctt tcgctgtagt ttgtatgcct
     9361 tcctgattct cgaaagctaa tactgcgata aatatagaca ttgtaaaacc gatacccgcc
     9421 agaaaacccg tacctatcaa gtgatgccac gtgacaccgt cgggcaatgc agccagatta
     9481 agttttgtgc cgatccagca aaatatcaga atgccgcaaa ctttgccaaa gaacaaacct
     9541 gtaaaaatac cgatgcttag cgttgaaaaa agtgcgaccc ataccgatgc atccatgaca
     9601 attaaggtgt tagccagtgc aaaaataggc atgataaaaa actgaaccgg tttgtgcagt
     9661 gcgtgctcca gagcaagtaa cggggaagat gtctcatctt tggaatacgg tatggtcatg
     9721 gctaaaatga ctccggcgat ggttgcatga atacccgact tgagtacgaa gtaccaaagc
     9781 agaattccca atccaagata gattgcaaga gagcgtatgc ctaatcgatt tatcaaaatc
     9841 aaaacaagta cagtcactaa agccgccgct ttatagttta aaaataatgt agatgagtaa
     9901 aacagggcga tcactatgat cgcgccaagg tcatcgatta tcgcaagcgc cgtgaggaaa
     9961 acttttatcg acacaggtat gcgagaaccc aataatccga gtatggctaa cgcgaaggcg
    10021 atatcagtgg ccatcgggac accccaaccg ttttcatata tcgtaccctg attgaagctt
    10081 acataaatta atgccggtac aagcatccca ccgactgcgc caaagagtgg cagactggcc
    10141 cgacgcagag aggaaagctc gccgctcacg atttcgcgtt tgatttcaag acctaccagc
    10201 agaaaaaaca ccgccatcaa tccgtcattg acccagtgca gaatggtttt atggagatca
    10261 atattttctg tagcaaatcc aatacgtgtg tgccagaatt gatagatggg ttcgttgaga
    10321 ggcgtattgg ctaaagccaa agacacaatc gtacaaaaaa taaggattat tcccgacgac
    10381 tgttcgtagt gaataaattc ctgaatggga tttaatattt ttttcatgtt gtcagatttt
    10441 actttcatta gcgaggtaca ggaacagtat tgagtacgtc ccgaatcatg tcatcggggc
    10501 gcagaccttc catttcggtt tccggttgta gcacaaggcg atgacgcagt acggcaggcg
    10561 ccagatcttt gacgtcatcc ggtatgacga aggtgcgatt agccaacgcg gcctgcgttt
    10621 tcgcggccaa aagcaagttg atcgtagcgc gtgggcttgc gccgatcatg atgccggagg
    10681 tttcgcgcgt tttgcgaaca atctctgtga tgtattttag tattccgtca tccactacga
    10741 tttgtcgaat caaatgacgt gcccgtgcga tcatttccgg tgtacacggt ttttctattt
    10801 ttatttggtc tatctgatgc gcatcgaagc cgccgtggta tgcgcgaagc acttgctctt
    10861 cctcactggc ggagggatac ggcacaataa gcttaaacat gaaacgatca agctgcgcct
    10921 caggcaaagg ataggtccct tcgtattcga taggattttg tgtagccatc accataaaag
    10981 gttccggtaa aagataacgc gtgccgtcaa tcgtaatttg tcgctcttcc attacttcca
    11041 gtaatgccga ttgcgtttta gccggtgcgc gattgatttc atcaatcagc gcaatgtggg
    11101 taaaaatagg accgcgtttg acattaaact gattggattt tggatcaaaa acattggttc
    11161 cgatcacgtc ggacggcatc agatcaggcg taaactgaat acgcttgaat tcacaagaca
    11221 acgctttggc taacgttttg gccagtagcg ttttggctat accgggtaca ccttccatca
    11281 agatatgacc gccgctcagc agtgcgatga tcatttcgcg tttgatatcc gactggccta
    11341 tgacggtagt atcaagagcg gccgaaatct gccgatacaa atcggctgcg tcattgagcg
    11401 gtgcatcgct atggggagtt actgtcaaat cggacatggt atatccttaa gattgggttg
    11461 gataatgtaa aaaatagatg tgatccaatt gttttgcacc ggcgatcaat tcttcatcgt
    11521 caatgcttac ctctcgtttt tgtacgggat cgtttttgag gtcgggcgat ttgacgacga
    11581 tcgtt
//
LOCUS       DAOQNQ010000076        11302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2045_length_11302_cov_5.175691, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000076 DAOQNQ010000000
VERSION     DAOQNQ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11302
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2045_length_11302_cov_5.175691"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..3186
                     /locus_tag="PK754_06595"
     CDS             154..3186
                     /locus_tag="PK754_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNB09207.1"
                     /translation="MLKLLFVFVTLLSLSLQAQYYDNYFGTNKVQYTHFEWNILETEH
                     FEIYYYPEMRELAERAGGLAEEQYAELQSRFNHALIKKVPMIIYSSHMHFQQTNTISA
                     ELPEGIGGFFEFFKGRVVLPSDGSTTHFRRVLRHELVHVFMNSKVNRIISQHRKFNHP
                     GPPLWFTEGLAEYWSGQPDFQAEMVIRDATLNGTLLPVQEFWRIDGSYYMYKLGENFL
                     HFLTENYGEEKVLQLMENIWKFDEFQKVVTHTVGKNYETLSQEWLYWLKKKYYPTLSQ
                     SDMPFAISQTITKVGFSSKPAYLFDGTNEEIFFVANRDGYTNIYRQNLKEAKAKVFIE
                     GERTHEFEAFHFFKSKIHVNEQGKIAFATKSGERDVLYVYNTQTGRKEHQYTYKDLTG
                     IISPHWAPDGKSIVFSGLHMSGNSDLYTVHVSTDETDGILTRITNDFYDDRDPAWSPD
                     GKTIVFSSDRAGFSPEGTYNLFIYDLETARTFQLTSGKYQDASPAWSPKGDYIVFTSN
                     RQGSPTNISVVPFIYQDQSFIETSTRGALQDSIYTHYAPRQLTDLTSAVFDPIWTKDG
                     DLVMTAFEEFSFKLRRMEKAYDKARQLVPTERKKIEGVRYPWTTPKVSIIGSNTRSYE
                     KKYSLDFVQGAFQIAPTYGYGGGATIGYTDMLGDDQYYLTVFNTGQTTSDILTDWNFI
                     LTKVNLGQRVKYAYGGYRFKGEFTQIGDQGLPETVHEERYGGFFQVAYPLSKFERIET
                     GISVSRYQRGNAFGALLPLDGVSIGNNVGYVYDNSLWGITGPIDGRRAMLSLGYTSDA
                     YKSQQNFYTIIADYRQYFRITTRSAYAFRTMTQWNDGKNPSNFVVGGSWDLRGFPRWN
                     MPGTRFFVMNHELRFPLMDAIQLRFPFVGLGFRSIRGAIFTDIGNAWGVQKTDGRVIL
                     GHQKFDGFLGSVGTGFRINFLGVMVLRFDFGKMFDARGYKFGKIRLPGNRDRFVETKL
                     NTNKRSEGFGGIDIDGNIIEKRRWSRGIFFQFWFGADF"
     gene            3186..4337
                     /locus_tag="PK754_06600"
     CDS             3186..4337
                     /locus_tag="PK754_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HNB09208.1"
                     /translation="MKTILVCIILIFQLMACGTTGNIRIAAYFKKSTTSPWSNIGGNP
                     GRTGYAINELTLPMQKVWTKKLSTSLGKAIVVSDSIIYTTTHDGRFYAIHSGSSQTVG
                     YIKFTHASQAGVAISGQKTAIGISNGKQNFITHQVFDGKFLTIKNTGAIETNPLIYDD
                     LAYAGTLSNTAVCVDMNNGETVWSYTTSAPVHASPTISGSAVYFGDDDGILFAFNRFN
                     GKLLWKLKLEGPILSAPVADDSLVYVASNDSTLYAIHQKEAVIAWHYRIGYDKPGNFY
                     ASPAVDEKLLYIGATDGRLYAFEKRSGNLVWMYETKGPISIAPVMSKSIVFIGSQDKS
                     IYALNKYTGVLEWQFETEGRIKSEMAIYGGSLFVGSENNMLYKFSGKLR"
     gene            4334..5158
                     /locus_tag="PK754_06605"
     CDS             4334..5158
                     /locus_tag="PK754_06605"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNB09209.1"
                     /translation="MNRHLQSLLYLFTLGILVPAMTYCQNKTPYGYLKIISHENPAFI
                     YLDNIAYEWENGKVFKLLVGRHTLAARRDNGLQTEQLDFLQNITINRDDTLSVYLRFE
                     KAITIQTKTPNSASVFINDSLYGKTPYLFIPETGNKYNVRISKTGHWDTTFTIVSSDT
                     PLMMGLRPKNHEMKSLNDNEFVNERWRKQGIHSYKKHLLIAGLSLIVTGYYTAANKKT
                     ADNAFEKAKLARRSGNIALSKKLTDKTRRYDDRARIGFIGLQISSAGFATLLLLSR"
     gene            5250..6476
                     /locus_tag="PK754_06610"
     CDS             5250..6476
                     /locus_tag="PK754_06610"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNB09210.1"
                     /translation="MKKFNIPLFYRSSIITAIKNYRRTLDQRKKDFSPTRLDFGPVEF
                     LIARHFGFCYGVENAIEISYKAIEENPGKRIFLLSEMIHNPEVNSDLQSRGVRFIMHT
                     HGEQIIPWADINRNDVVLVPAFGTTIEIENKLKDIGIQSYTYDTTCPFVEKVWNKAAQ
                     IGKESYTVVVHGKPSHEETRATFSHSRQGAPTVVIRDMKEAVLLGRFIIGELPPEAFY
                     QSFENQYSEGFDPGKDLKRIGVVNQTTMLASDTQAIADYLRNLMIDKYDLDQASVHLH
                     FAETGDTLCYATNDNQTSTYGLLSEKADLAVVAGGYNSSNTSHIVELCEEKLPTFFIS
                     SAEKIIDSQTIMHWDFHHKIEKTSKNFIPQKEKVTILLTSGASCPDTIVEGILLRLLT
                     FFSNTRDFDDVMRRFQ"
     gene            6617..9655
                     /locus_tag="PK754_06615"
     CDS             6617..9655
                     /locus_tag="PK754_06615"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB09211.1"
                     /translation="MVKGLPVIEHYAPQTYGAGVDNFCFVQDKRGFIYVANGSGVLEY
                     DGIQWRLIPLENRAAASWLTISPEGTIFVASLGEFGCLVPDSSGFLKYQSLVNRQTHP
                     QLKFQTFWEMCASSQATYLRSYRHLVRYKNGNIKVWSADGDGFDIMGMVYDTIYTRIK
                     HETIVRIIGDSLVPLPGGDFFKEIKINGFLPYQKNKLLIATRKDGLFIYEAGQIKSLK
                     TEADDYLKKYFVYDTRQLPDGGYVIATLQGGVVLFDADLRVKQIIDETSGLPANGVNS
                     VFVDNNFNLWLGHSYHGITRIEIHTPLTLLNASTGINGRVKRMLRHKGTLYVMTNSGL
                     FRLTEKGNAYHFELLHSGAPYYFDMFVTQGRLLISTGSGILEVKNDNVVSHTSVYTYA
                     VLNSNRDSNIVYASEGLGMTIYRVGKSEKWELVKTIPSVKVAVRTMAYDEYGDLWLGT
                     EYEGLGRMQINKKDHLPDTVEFFDSKFGLPAGEISVWSTGAAPLFSGHDGFYKFSRQY
                     HRFNKLSETEMAMHRDNSEYWIGLNQGRWMITPTALQIMPEKTIINRLRAIKNAVDFI
                     YWRDSDGTEWYGGDFLIVKHKPKTQTLLPERPTVVLRRVMQGLEVIFSDDEIKRNHQI
                     VFESEPTQPIRFEFTAPVFEMRPQFQTRIIGLNEQWESWGSDPVRLYERIPAGKYRFE
                     VRARDHFGRISEITGADFVVNPPWYLARTMLMLFALVAIALVWLFIRYREHRLKQMNQ
                     ELERLVEDRTRALKAKEAQLIQSEKMAALGQMVGGVMHEINNPLTFIQPNLEYVQLNL
                     KKFISDITANAVTENRQAEIREFERDTINALKSSVKGAERIRDIVSSLKSFSAYGKNQ
                     VMDVRLDHQLSGVVDLIFNQNNDIRFIRSFKDTRFVRIDTMEVMQCVVGVLNNAVAAV
                     HDALAEKILLHENAEIRICTMDGPDQTVIIEIEDNGIGIPETVLPRIFEPFFTTRMIG
                     KGKGLGLSEAYAVIKKHHGNIEVTSKPQKGTRVIITLPAN"
     gene            9762..10889
                     /locus_tag="PK754_06620"
     CDS             9762..10889
                     /locus_tag="PK754_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNB09212.1"
                     /translation="MTWILLFICFVCSDSIAQTLTFEEYEPPSTLVVPENFRYQSKYP
                     FIDVHNHQFEMPTQDLKYLTKQMDSLNMAVMVNLSGRGFKREQNKDGSFRFGLQDGSY
                     LKGAIENARKNVPGRLMVFTNINFGGIDDPGWSAEAVRQLEEDVKNGACGLKIYKNLG
                     MSFKDSSGNIIAVDDPRIDPIWKKAGELGIPVLIHSADPFQFWQPWDKNNERWLELKE
                     RPDRKRDSSKEPSFESIIAAQHRVFKKHSKTIFINAHLGWMGNDLAKLGKLMDSLPNM
                     YTEIGAVLAEIGRQPRQARDWFIKYQDRVLFGKDSWNPQEYSTYFRVLETNDEYFDYY
                     RRRHAYWKMYGLGLPDDVLKKLYYKNALRIIPGIDKSLFPK"
     gene            11123..>11302
                     /locus_tag="PK754_06625"
     CDS             11123..>11302
                     /locus_tag="PK754_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09213.1"
                     /translation="MRRTMLLLFVCIATMAHAQDLPEIDGFIREGEWSDATVFSDFHL
                     ISPRSTEKYYDSTIVY"
BASE COUNT         3312 a         2279 c         2489 g         3222 t
ORIGIN      
        1 aaaacatatt gcagattttg acggtttgga cattccggca gtaataaatt gtaatatttt
       61 tctgacggac ggtacttact actgttggta cggtacacgt acggcccgag caatagagta
      121 tatagaccaa aacatcatac atcggattgt tacatgctaa aacttctctt cgtttttgtg
      181 acgctattat ccctctcatt acaggcgcaa tattacgata attattttgg caccaataaa
      241 gtgcagtaca cgcattttga gtggaatatt ttagaaacgg aacattttga gatctattat
      301 tatccggaaa tgcgcgaact cgctgaacgt gccggcgggt tggctgaaga gcaatatgcg
      361 gagcttcaat cccggtttaa tcatgctctg attaaaaaag tgccgatgat tatttatagt
      421 tcgcacatgc attttcaaca aacgaatacg atatctgccg aattacccga aggcataggt
      481 ggtttttttg aatttttcaa aggtcgggtc gtattgccga gtgacggttc caccacgcac
      541 tttcgcaggg ttttaaggca cgaattggtt cacgttttta tgaattcgaa ggtgaaccgg
      601 attatcagtc agcatcgaaa atttaatcat cccggccccc ctttgtggtt caccgaaggt
      661 ttagcggaat actggtcggg tcaacccgat tttcaggctg agatggtcat acgcgatgcg
      721 acgctcaacg gcacactgtt gccggtacag gagttttggc gcattgacgg ttcgtactat
      781 atgtataaac tcggtgagaa ttttctacat tttcttactg aaaattatgg tgaagaaaaa
      841 gttctccaac tcatggagaa tatttggaaa tttgatgagt tccaaaaagt cgtaacacat
      901 acggtcggaa aaaattacga gaccttaagt caggaatggc tctactggct caagaagaag
      961 tattatccca cgttatccca aagcgacatg ccttttgcga tttcgcaaac cattaccaaa
     1021 gtaggattca gttcgaagcc ggcctatctt tttgacggca ctaatgaaga aatctttttt
     1081 gtggccaatc gtgacggtta taccaatata taccgtcaga atcttaagga agccaaggca
     1141 aaagttttca tcgaaggcga aagaacgcat gaattcgagg cgtttcattt ctttaaaagt
     1201 aaaatccatg tcaatgaaca agggaagata gcttttgcaa ctaaaagcgg tgagcgcgac
     1261 gtactgtatg tttataacac acaaaccggt agaaaagagc atcaatacac ctataaagat
     1321 ttgaccggaa ttatttcacc gcattgggcg cccgatggca agtccattgt cttttccggg
     1381 ctccatatgt cgggtaatag cgacttgtat accgttcatg taagtacgga tgaaaccgac
     1441 ggtatcctga cacgcatcac caatgatttt tatgatgatc gtgatccggc atggagccct
     1501 gacggtaaaa ctatcgtttt tagttctgat cgcgcgggtt tttctccgga aggaacatat
     1561 aatttattta tctatgatct ggaaaccgcg cggacatttc agctaaccag cggcaaatat
     1621 caggacgctt caccggcatg gtcacctaaa ggtgattaca tcgtttttac aagtaaccgc
     1681 cagggaagcc cgactaatat ttcagttgtt ccgtttatat atcaagacca atcatttata
     1741 gaaacatcaa cgcggggcgc attacaagat agtatttaca cgcactatgc accgcgtcag
     1801 ttgaccgatc tcacctcggc ggtttttgat ccgatttgga ctaaagacgg tgatcttgta
     1861 atgaccgcat ttgaggaatt tagtttcaaa ttgcgacgta tggaaaaagc gtatgacaaa
     1921 gcgcgtcagt tggttccgac cgaacgaaaa aaaatagaag gtgtgcgtta tccgtggacg
     1981 acaccgaaag tttcgatcat cggaagtaat acccgctcgt atgaaaagaa atacagcctc
     2041 gatttcgtcc aaggcgcttt tcagatcgca ccgacgtacg gctatggtgg cggcgccaca
     2101 ataggatata ctgacatgct aggcgatgat caatattacc ttaccgtttt taatacaggc
     2161 caaaccacat ccgatatttt aacagattgg aattttattc tgaccaaagt taatctaggg
     2221 caacgtgtaa aatatgctta tggaggttat cgttttaaag gcgagtttac tcaaatcggc
     2281 gatcaaggtt tacctgaaac cgtacatgag gagcgctatg gcggcttttt tcaggttgcg
     2341 tatccattat ccaaatttga acggatcgaa acgggtattt cagttagtcg ttatcaacgc
     2401 ggcaatgcgt ttggtgcact gttgccgctg gatggggtaa gcatcgggaa taatgtcgga
     2461 tatgtttacg acaattcact atgggggatc accggaccga tagatgggcg ccgcgccatg
     2521 ctatcgctgg gttacacgtc ggacgcgtat aaatcgcaac aaaatttcta cacgatcatc
     2581 gccgattatc gtcaatattt tcgtattaca acgcgatcgg cttacgcatt tcgaacgatg
     2641 acgcagtgga atgacgggaa aaaccctagt aatttcgtcg tcggaggtag ttgggattta
     2701 cgtggtttcc cacgatggaa tatgccggga acgcgttttt ttgtgatgaa tcatgaattg
     2761 cgatttcctt tgatggacgc tattcaacta aggtttccgt ttgtcggcct aggtttccgt
     2821 tcaatacgag gagctatctt tacagacatc ggaaacgcct ggggcgtaca aaagaccgat
     2881 ggtcgagtga tactgggtca tcaaaaattt gacggttttt tgggtagtgt aggtacgggg
     2941 ttccgtatta attttctagg tgttatggtt ctgcgttttg attttggaaa aatgtttgat
     3001 gcgcgcggtt ataagttcgg taaaattcgt ttgcccggta atcgcgatcg atttgtcgaa
     3061 accaagttga ataccaacaa acgatcggaa ggttttggcg ggatcgacat tgacggaaat
     3121 ataattgaaa aaagacgttg gtcacgcggt atatttttcc aattttggtt tggagctgat
     3181 ttctaatgaa aacaatcctt gtatgtatca tcctgatctt tcaattgatg gcttgcggca
     3241 ctaccggcaa tattcgcatt gcggcttatt tcaaaaaatc cacgacttcg ccatggagta
     3301 atatcggcgg aaatccaggt cgaaccggat atgctataaa tgaattaact ttaccaatgc
     3361 agaaagtttg gactaaaaaa ttatctactt ctctcggcaa ggccattgtc gtatccgaca
     3421 gtatcattta tacgacaacg catgatggtc gtttttatgc gattcattca ggttcctcac
     3481 aaacagtcgg ttacattaag tttactcatg catcacaagc cggtgtggcg atatccggtc
     3541 agaaaacagc catcggcatt tctaacggga aacaaaattt catcacacac caagtttttg
     3601 acggaaaatt tttaaccatc aaaaataccg gtgccatcga aacaaatcct ttgatatatg
     3661 acgatctggc gtatgccggt acactgagca atacagccgt ttgcgtggat atgaataacg
     3721 gggaaaccgt ttggagctac acaacatctg caccggttca cgcttcacct acaatctcgg
     3781 gttcggcagt atattttggg gatgacgacg gaatactttt tgcatttaat cgcttcaatg
     3841 gcaagttgct ttggaaactc aagctcgaag gaccgattct ttctgcgccg gtcgcagatg
     3901 atagcctggt gtacgttgca tctaacgata gtacattgta cgcaatccat caaaaggaag
     3961 ccgtgatagc ttggcactac cgcataggat atgataaacc cggtaatttt tatgcttcac
     4021 ctgcggtgga tgaaaaactt ttatatatcg gcgctaccga cggacgtctg tacgcgtttg
     4081 aaaaacggag cggtaattta gtatggatgt atgaaactaa aggtcccatt tccatcgcac
     4141 cggtaatgag caaatcgatt gtttttatcg gatcgcaaga taaaagcata tacgccctca
     4201 ataaatacac tggagtcttg gagtggcagt ttgaaacaga aggacgaatt aaatctgaaa
     4261 tggcgattta cggcggttca ctttttgtcg gttctgaaaa taacatgctt tataaattta
     4321 gcgggaaact cagatgaata ggcacttaca gagtttgttg tatcttttta ctttagggat
     4381 tctcgttccc gctatgactt attgtcagaa caaaactcct tacggttact taaaaataat
     4441 ttctcatgaa aacccggcct tcatttattt agacaacatt gcatacgaat gggaaaacgg
     4501 taaagtattc aagcttcttg tcggacgaca caccctcgcg gctcggcgag ataatggact
     4561 tcaaacggaa caattggact ttttgcaaaa cattaccatc aacagagatg atacgttgtc
     4621 tgtatatttg cgtttcgaaa aagccataac gattcaaaca aaaacgccca actcagcatc
     4681 tgtttttatc aacgattcgc tatacggtaa aacaccgtat ttatttatac ctgaaacagg
     4741 gaataaatac aatgttcgca tcagcaaaac aggacattgg gatacgacat ttaccatagt
     4801 ttcaagcgat acgccattaa tgatgggatt aagaccgaaa aaccatgaga tgaaatcgtt
     4861 aaacgataat gaatttgtta acgaacgatg gcgcaaacaa ggtattcata gttataagaa
     4921 gcacctgctc atagcgggtc taagcttgat tgtcactggg tactatacag cggccaataa
     4981 aaaaacggcg gataacgctt ttgaaaaagc gaagttagcc aggcgatcgg ggaatattgc
     5041 gttatcaaaa aaattgacag ataaaacccg ccgttatgat gatcgcgccc gaatcggatt
     5101 tatcggctta caaatcagtt cagcaggatt tgccacgctg cttttactaa gtcgctaatc
     5161 atataaaaaa acatttttta aatacggtgt gacacgctat attcagtcag tatatcattg
     5221 atttagataa actgacgctt cacccgttca tgaaaaaatt taatataccg cttttttacc
     5281 gcagctctat tatcacggca ataaaaaatt accggcggac gctagaccaa cgaaaaaaag
     5341 atttttctcc tacacgtctt gatttcggtc cggtagagtt tcttattgca cgccattttg
     5401 gtttttgtta cggcgtagaa aacgccatag aaatttcata caaagccatt gaagaaaacc
     5461 ccgggaaacg tatttttttg ctcagtgaaa tgatacacaa tcccgaggta aattcggatc
     5521 tgcaaagccg gggagttcga tttatcatgc acacgcacgg agagcagatc attccgtggg
     5581 cggatatcaa tagaaatgac gttgttttag tgcccgcgtt tggtacgacc attgaaatag
     5641 aaaacaaact caaagatatt ggaattcaat cgtataccta cgatacgaca tgtccatttg
     5701 tcgagaaagt atggaacaaa gcagctcaaa tcggcaaaga aagttatact gtcgttgttc
     5761 acggaaagcc ttcacatgag gaaacacggg cgactttttc tcatagcagg caaggagcgc
     5821 ctacggttgt tattcgcgat atgaaagaag ccgtattact cggacgattt ataatcggtg
     5881 aattgccacc ggaagcgttt tatcagtcat ttgagaatca gtactccgag ggatttgatc
     5941 cgggtaagga tcttaaaagg atcggtgtgg tcaatcaaac aacgatgctg gccagcgaca
     6001 cccaagccat cgcagattat ttgcgcaatc ttatgattga taaatatgac ttggaccaag
     6061 catcggtaca tctgcacttt gccgaaacag gcgatacatt atgttacgcg accaacgaca
     6121 atcaaacttc cacgtatgga ttgttatcgg aaaaagcaga cttagcggtc gttgcgggtg
     6181 gatacaacag ttccaatacg tctcacattg tcgaattatg tgaagaaaag ttaccgactt
     6241 tttttatttc atcggccgag aagatcatcg actcccaaac gataatgcat tgggattttc
     6301 accataagat agaaaaaact tctaaaaact tcattcccca aaaagaaaaa gtcaccatac
     6361 tgcttaccag tggagcctcg tgtccggata cgatcgtcga gggaattttg cttcgtttgt
     6421 tgactttttt ttcaaataca cgcgatttcg atgatgtgat gcgtcgcttt caatgaaggt
     6481 tctcaagtta gcgtaatacc gctaacatat tttcatatgt ttaatctttt ataaagatcc
     6541 cgtgatcaaa tctgtaataa catattgcgg gcttactttt ttatttgttt tcggcgcggc
     6601 gatatatagc caaaacatgg taaaaggttt gccggtcatc gaacattatg ccccacaaac
     6661 atacggagcg ggcgtagaca acttttgttt tgtccaagat aagcgtggtt ttatttatgt
     6721 tgccaatggc tccggcgtac tggagtatga tggcattcaa tggcgattga tcccgttaga
     6781 aaatcgtgcg gccgcctcat ggcttacgat cagtccggag gggacgatat tcgtggccag
     6841 tctcggtgaa tttggttgct tagtgcccga cagttccggt tttttgaagt accaatctct
     6901 tgttaatcgt cagactcacc cgcagttaaa atttcaaaca ttttgggaaa tgtgcgcctc
     6961 gtcgcaagcg acgtatttgc gtagttatag gcatttagtg cgatacaaaa acggcaacat
     7021 caaagtgtgg tctgccgatg gagacggatt cgacatcatg ggtatggtgt acgatacgat
     7081 atacactcgt atcaaacatg aaacaattgt tcgaatcatc ggtgacagtc ttgtaccatt
     7141 gccgggcggt gacttcttta aagaaataaa aatcaacggc ttccttccat atcaaaaaaa
     7201 caaacttctt atcgcaaccc gtaaagatgg tctttttatc tacgaagcag gccaaatcaa
     7261 atctttgaaa acggaagcgg acgactattt aaaaaaatat tttgtttacg atacgcgcca
     7321 attgccggat ggtggttatg tcatcgcgac cttgcaaggc ggcgtggtgt tattcgatgc
     7381 ggatttgcgt gtcaaacaaa taattgatga aacatccggt ttgcctgcca acggtgttaa
     7441 ttcggttttt gtggataata attttaatct gtggctaggc cacagctatc acggcattac
     7501 gcgaatcgag atacatacgc ctctcacttt gctcaatgcc tcaacgggta tcaatgggcg
     7561 cgtcaaacgc atgcttcgcc ataaaggcac gttatatgtg atgaccaata gcggtttatt
     7621 tcgattgacc gaaaagggga atgcatacca ttttgaatta ctgcattccg gtgcgccgta
     7681 ttattttgat atgtttgtta cgcaaggacg actgttgatc tctaccggaa gcggtatttt
     7741 agaagtaaaa aacgataacg ttgtgtcaca cacttcggta tatacgtatg cggtgttaaa
     7801 tagcaatcgg gattctaaca tcgtctacgc gagcgaaggc ctaggtatga cgatttatcg
     7861 tgttggcaag tctgagaaat gggaacttgt aaaaacaata ccttccgtca aagtagccgt
     7921 tcgaaccatg gcgtatgacg aatacggtga cctttggctg ggaacggagt acgaaggtct
     7981 cgggcgtatg caaatcaata aaaaggacca tctgccggat acggtcgaat tttttgatag
     8041 caaattcgga ttgcctgccg gtgaaatttc agtttggtcc accggtgcgg cgccattatt
     8101 ctcaggtcat gacggatttt ataagttttc ccgtcagtat caccgtttca ataaattatc
     8161 cgaaactgaa atggcaatgc atcgagataa tagcgaatat tggattggtt tgaaccaagg
     8221 acggtggatg atcacgccaa cggctttaca aataatgcct gaaaaaacca tcatcaatag
     8281 attacgcgct ataaaaaatg ccgttgattt tatatattgg cgcgattcgg acggtacaga
     8341 atggtacggc ggtgattttc ttatcgttaa acataaaccg aaaacccaaa cccttttacc
     8401 cgaacggcct acggttgtac ttcgccgcgt tatgcaaggc ttggaagtga ttttttcaga
     8461 tgatgaaatc aaaaggaatc atcagatcgt ttttgaatcc gaacccacgc agccaattcg
     8521 ttttgaattt actgcgcctg tttttgaaat gcggcctcag tttcagaccc gcattatcgg
     8581 cttgaacgaa caatgggaat cctggggtag cgatcccgtt cgtttgtatg aacgtatacc
     8641 tgccggcaaa tatcgttttg aagtcagggc tcgcgatcac tttggacgta tttcggaaat
     8701 aaccggcgct gactttgtcg tcaatccgcc atggtatctt gcacgtacga tgctgatgtt
     8761 atttgctttg gttgctatcg ccttagtttg gttgtttatc cgttatcggg aacaccgcct
     8821 taagcagatg aatcaggaac ttgaacgcct tgttgaagat cgtacgcgtg cacttaaagc
     8881 taaagaggcg cagctcatcc agtccgaaaa aatggctgca ctcgggcaga tggtcggcgg
     8941 agtaatgcac gaaatcaaca accctttaac atttattcag cctaatcttg agtatgtgca
     9001 gcttaattta aaaaaattta tcagtgatat caccgccaac gctgtaactg aaaatcgtca
     9061 ggcggaaatt cgcgaatttg agcgcgacac cattaacgca cttaaaagtt cggtcaaagg
     9121 tgccgaacgc atacgcgata ttgtatccag tctaaagagt ttcagcgctt acggtaaaaa
     9181 ccaggtgatg gatgttcgtt tggatcatca actatcaggt gtggtggatc ttatttttaa
     9241 tcaaaataac gatattcggt ttatccgttc atttaaagat acacgatttg tgcggattga
     9301 taccatggaa gtaatgcaat gtgtggtcgg cgttctaaat aatgccgtcg ccgctgtaca
     9361 tgatgcactg gctgaaaaaa tattattgca cgaaaacgct gaaatccgca tctgtactat
     9421 ggacggtccc gatcaaacgg ttattataga gatagaagat aatggtatcg gtattcctga
     9481 aactgtactt cctaggatat ttgagccttt ttttacgacg cgtatgatcg gtaaaggaaa
     9541 aggactcggt ctctcggaag cctatgccgt aattaagaaa catcatggta atatagaagt
     9601 aacgagtaag cctcaaaaag gtacgcgcgt aattatcacg ttgcctgcta attgatatta
     9661 tcaaatttat tggaaccttg taatataatc tttcgtacaa cattcattcc taattcggta
     9721 tttaattttt tgattaataa acggagatac gcatgcgtct aatgacttgg attttattgt
     9781 ttatctgttt tgtttgtagt gatagcatag cccaaacgtt aacctttgag gaatatgaac
     9841 cgccttctac tttggttgtg cccgaaaatt ttagatacca atctaaatat cccttcatag
     9901 acgtacacaa tcatcagttc gaaatgccca cgcaggattt gaaatacctg accaaacaaa
     9961 tggacagtct taatatggcc gtgatggtca atctgagcgg gcgtggtttt aaaagagaac
    10021 aaaataagga cggttcattc cgtttcggac ttcaagacgg aagttatttg aagggagcta
    10081 tagaaaacgc ccgtaaaaat gttcccggtc gtttgatggt ctttaccaat atcaattttg
    10141 gcggcattga cgatccgggc tggagtgcag aagccgtacg tcaattggaa gaagatgtta
    10201 aaaacggcgc ctgcggtttg aagatatata aaaacctcgg tatgtcattc aaagattcga
    10261 gcggcaatat catagctgta gatgatccgc gcattgatcc tatctggaaa aaagcggggg
    10321 aattgggaat accggtactt attcactccg ccgatccatt tcagttttgg caaccctggg
    10381 ataaaaataa tgagcgatgg ctcgaactca aagagcgtcc tgatcgtaaa cgagattcga
    10441 gcaaggagcc ctcatttgag agtattattg ccgcacaaca tcgcgttttc aaaaaacatt
    10501 caaaaactat ttttattaac gcgcatttag gatggatggg taatgacctt gcaaaactcg
    10561 gcaagctcat ggattcactt cccaatatgt acacggagat cggtgctgta ttggcagaga
    10621 tcggccgtca accgcgtcaa gcgagagact ggtttatcaa atatcaagat cgtgtgcttt
    10681 tcggtaaaga ttcatggaat cctcaggagt attccactta tttcagagtt ttagaaacaa
    10741 acgatgaata ctttgattat tatcgtcgtc gtcatgctta ttggaaaatg tatggtctcg
    10801 gacttcctga tgatgtctta aaaaaattat attataaaaa cgcgttacgc attataccgg
    10861 gcatagacaa atcgttattt cctaaataga agcattgaat tttataattt gaacattcaa
    10921 aagcatcgcg ataacaacgc gatgcttttt tgtataaaaa cggttatttc ttacgtttat
    10981 tgaaactttt tattaaaacc accgtttatc ctcccaaccg acagctttcc ttatagtttg
    11041 ttttcattac cgatcaaata agtgtcttac tgaatcgtct gaatttgtac ataacactac
    11101 aacttatacc aaaggatacg gtatgcgccg cacaatgctt ctattatttg tatgcattgc
    11161 aacaatggct cacgcacagg atttaccaga aattgacggc tttataagag agggagagtg
    11221 gtcagacgca accgtttttt cagattttca tttgatttca ccccgatcta ccgaaaaata
    11281 ttacgatagc acgatagtct ac
//
LOCUS       DAOQNQ010000077        11253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2052_length_11253_cov_4.748973, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000077 DAOQNQ010000000
VERSION     DAOQNQ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11253
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2052_length_11253_cov_4.748973"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..503)
                     /locus_tag="PK754_06630"
     CDS             complement(132..503)
                     /locus_tag="PK754_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005344690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB09214.1"
                     /translation="MNHSPGFLKVVNDSKTRVQEVSVQQCLDDVQKNPAIALIDVREE
                     SEYAAGHVSGAEHIGRGIIERDIETKHPDHDQVLYLYCGGGFRSALAADNLQKMGYTR
                     VYSVAGGWRAFLDIQAPVEKP"
     gene            complement(512..1297)
                     /locus_tag="PK754_06635"
     CDS             complement(512..1297)
                     /locus_tag="PK754_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09215.1"
                     /translation="MATEPTFDLYLFSTHEETVKSALQAGVKGIGINWEHIGKSSTDG
                     TVTHDSIDDLTKIRAITSAPILCIINRFSPGTEREVEEAIKGGATEILLPMVTESSQI
                     EKTLQWANGRVGVGIVIETQAALRSARQLARYPLSRVFIGLKDLAEENRRTGKNTHIY
                     EPIVDGTIERVRSGFKMPVGFGELTLPECGTPIPSRLLMSEMARLQCSYGVLKQAFLQ
                     DIKDKSMADEVPRIIRAIQDTFAYSQTVFGLNRRALTSMLSQK"
     gene            complement(1424..2506)
                     /locus_tag="PK754_06640"
     CDS             complement(1424..2506)
                     /locus_tag="PK754_06640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB09216.1"
                     /translation="MRFVFPGLPVKNNADLMTQLNRGAERVAERLKTTDWSAVPVSDY
                     NKRYLKKYTAKLTPTFQKYVYILALALEKQNDLARCTLVDFGGGCGLLSCIAKAAGVG
                     RVVYNDIFETSVQDVRKLSEIWRIPINDFVCGDLDTVHQFLEREKINCDAFVSCDVLE
                     HIYRHELFFKQAAELPSKAMSLVMSTHANPLNPAIRRSLQASQRRVELEDRKAEWGHK
                     DRDDLRSYRRIRAEMISAENADLEKEMIYELAERTRGMKAEDIRATVKIFKTEGRMPP
                     KPDHPTNTCDPGNGNWADRLLEPQVYAQALKEAGFQTTVYSGFYGHPHPTGRRTIARL
                     LDIGIRWLGHHQGVRLASYFIVKGVK"
     gene            2680..3789
                     /locus_tag="PK754_06645"
     CDS             2680..3789
                     /locus_tag="PK754_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNB09217.1"
                     /translation="MHATTDDRKSYNRQKLWLSLLSGVLSFCVLIALFFSGLSDTLAR
                     WVMSQTERPYLQFLLYLFALGLLQSIVTFPLSFYNGYILEHRYRLSNQSFKQWCFENV
                     KGLALGIVIGVPLLLIFFYTLRNFGFWWWLPLAGILFLFSVLLARIAPTLIFPLFYTF
                     KPVEKTALVERITRLCLKAGLQVQGVFEFNLSKTTKKANAAFAGIGKSKRILLGDTLL
                     QQFSDDEIEVVFAHEAGHYAHSHITKGMIWGIVSTFGGLCLTAFIYDNLFPAFEYTDR
                     AELGALPLIAILLSLYSVITTPAGHILSRRHEREADAYALRETKNRAAFISAMQRLSD
                     TNLADENPHPLVEWYFYSHPAIGKRIAAAETMILE"
     gene            3795..5723
                     /locus_tag="PK754_06650"
     CDS             3795..5723
                     /locus_tag="PK754_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNB09218.1"
                     /translation="MLQLVNLTVEFGGRTLFSNLTWKINDRDRVALIGPNGAGKSTLL
                     KIIARQQSATSGTIAQPKHLRVGYLPQDGLHAHGKPLLEEVVSAFDEIVRIKQRLDEL
                     EHALHTLDHEAPAYARALDEYGHLHQRLDDMGAASAESKAAKILNGLGFKESEFDKLC
                     ELFSGGWQMRIALAKLLLQNPDVLLLDEPTNHLDIDSIEWLEEYIKNYEGAIFMVSHD
                     RYFIDNVCNRIVELERQTLTDYVGSYDDYEEQKALAEEQLLAAYERQQDEIKRVQLFI
                     DRFRYKATKARQAQSRVKMLERMDRIQLPEEEKRAIQFRFPQPIKSGRVVLEIAGIQK
                     SYDGRPILKGIEFAIERGEKVALVGPNGAGKSTLLRIIAGDETPDTGTIKHGYNVTME
                     YFAQQQSDKLDMTRSVYEEIASAATNQPPLLLRTILGAFLFSGDDINKKVKVLSGGER
                     SRLAFAKMLLNPANFIILDEPTNHIDAQSKEILQGALTDYEGTLLIVSHDRYFLESLV
                     TKVIEVREGHAKIYPGTFAEYHERKIKEHAEAAARESIAEVKVKPIASKPEREVKKEP
                     VKADQQAKRKREKQLSQIESDIQKAETQKIEIEALLTDPLLYKNPDRQKTVQSEYNAL
                     HARITALYEEWNALAAEL"
     gene            complement(5885..6571)
                     /locus_tag="PK754_06655"
     CDS             complement(5885..6571)
                     /locus_tag="PK754_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNB09219.1"
                     /translation="MMLASLRLGLYWLADFLYPSTCLACRVRLDPADKHICKSCWNAL
                     KTVGESDYEGIIRCEAIDVIHAGYYYDEVLQKSIHALKYDRALSLIPKFADRLAQIVH
                     TNPMLKTATRITAIPLNPIRKRERGYNQAELLARAVGQLAGLSYVETLQRVRHTESQT
                     KMNTAEDRIKNVDGVFIAMADASVQDQSVILVDDIITTGSTANACAAALKKAGASKVF
                     VLTVGRPTLG"
     gene            complement(6568..6921)
                     /locus_tag="PK754_06660"
     CDS             complement(6568..6921)
                     /locus_tag="PK754_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09220.1"
                     /translation="MILLFIEDILFLSKIKTQARALGIQTVVADKANENVRELFLAQS
                     YRKIFVDLNIQKRDPFALIRLLKADSVAMSIPIIAFFSHVQTELAHKASEAGCDTVMP
                     RSQFSARLNDLLTGV"
     gene            complement(6918..8066)
                     /locus_tag="PK754_06665"
     CDS             complement(6918..8066)
                     /locus_tag="PK754_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB09221.1"
                     /translation="MKKQHHQILVVDDEELNVRFLSQFLIRKGFAAITAHSGEEALAL
                     VASESPDLVLLDAMMPQMDGFEVCRRLRANPETALLPVVMVTALQRPEDEAHALDAGA
                     DDFLPKPINYTELMSRIRSLMRIKDLHDELRTNQDALEEKHDQLLQLQHLRESLTQML
                     VHDLKNPLTGIMGCTELLTMLSENMTEKQISILKKLEDNTGTLLKMVTELLDVSRMEE
                     NKLVIRPEAVSVKEVFMHNIGELEYMIEKYQLVMDMHLSDDNAMVWADRDLLMRIIAN
                     LLFNAIKHSQKGGKLTLTCEWQAAEHRMCFAVTDTGEGIPVEYLEKIFEKFSQVDLKQ
                     RGLKSDRGLGLTFCKLAVEAHGGKIWAESELGKGSTFKFFIPSSAPSA"
     gene            8220..8948
                     /locus_tag="PK754_06670"
     CDS             8220..8948
                     /locus_tag="PK754_06670"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNB09222.1"
                     /translation="MRRLFYSAFYFCVIVLFFVLLLDKWLMPMYVDLGKDIAVPAVTG
                     LSSEMAQKKLSELGFSVVLKRRYDISVAPKTVIQQSPEPQALVKAGRQIHLVIADDNQ
                     TVIMPSLTLSTYRDAVFNLQSIGLAIGVLDSASSNEFPQGVILKQSIEPNTVVRLGQS
                     VDLTISLGNNITESKVPFLINLSLKDAKARISDSGLRLGKIERKFLPDLIPDTVVGQS
                     LDSSLVVTPLTIIDLTVSSTDSEQ"
     gene            9028..10086
                     /locus_tag="PK754_06675"
     CDS             9028..10086
                     /locus_tag="PK754_06675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB09223.1"
                     /translation="MVQTYIIGKELSNRNWEVHYVAYNKSGMRGYMRHEGLHVHWIKA
                     RRYLPILSLPSVWMALIRINPDVLYQRGRDILTGLMALYGLMYGKHTLWASAGETGVE
                     RKKYASQKNRQKGNFIRRWVLQIEALINDTICNFGMRRTDQMIVQTAYQQQRLLVTYA
                     RSSTIIKSGHPLPPSVQRALPIKVLWISSIKPVKHVDLFLDLADMCKNEPIEFWIAGQ
                     IVHEETGHKMQERIAKMNNIRYLGAVPFEQSSALIASSHILVNTTEVGYEGLPNAFVQ
                     AWLAGTVTLSLHTDPDAVIMKNDLGYVNPELVQLKEKMLRFIIEHAVWLRQSNACRQY
                     AVENYSVVSIVNQIEKTL"
     gene            10101..10790
                     /locus_tag="PK754_06680"
     CDS             10101..10790
                     /locus_tag="PK754_06680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB09224.1"
                     /translation="MSSISEQKHQRVVELENELRSENFKRPQYRYGMDLVKATGLTKG
                     RIIEFGGGRGEFSRLLKDHGFEVVFTDINQANVEFAKSIGFEGYVIDANSGMADQKAD
                     SYDGAVMLEVIEHVTQAELLLTETHRILKPGGFLVLSTPNPYFLWRRLSVLFGKPIDG
                     EGYHWRYFTVYSLESTLRESGFTIITHNHYASTFGLGKILKLIGYTKTFRWPRFLHGL
                     LVRKIYFLAKK"
     gene            10851..>11253
                     /locus_tag="PK754_06685"
     CDS             10851..>11253
                     /locus_tag="PK754_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNB09225.1"
                     /translation="MKGMILAGGAGSRLYPISKVYSKQLMYVYNKPMIYYPLSTLILG
                     GIKDILIITTPDDAPMFKKLLGDGKHIGLNLQYAEQPKPEGLAQAFIIGESFINNEAV
                     SLILGDNLFYGYLDFFKRAIQNTKVGATIFGY"
BASE COUNT         3052 a         2643 c         2484 g         3074 t
ORIGIN      
        1 tgatacaaag gacggtactg cttgtggttg gcgtaattct gttcagccat attttcctat
       61 ttcttatgtt ttaggagaac cttatttgat aatacgcaac gtccgatcaa aaatgcaact
      121 gcaacttaaa tttatggttt ttcgaccggt gcctggatat ctaaaaatgc acgccacccg
      181 ccggctacgg aatacacccg cgtataaccc attttttgca gattatccgc tgccagcgca
      241 gaacgaaaac caccgccgca atagagatac aatacttgat cgtgatccgg gtgtttggtt
      301 tcgatatctc tttcgataat accgcgcccg atatgttccg caccggaaac atgacccgcc
      361 gcatattcgc tttcttcacg cacatcgatc aatgctatcg cgggattttt ttgaacatcg
      421 tcaagacact gttgtaccga aacttcttgc acccgtgttt tggaatcatt tacaactttt
      481 agaaagcccg ggctgtggtt catacgacct cttatttttg agataacata ctggtcaatg
      541 cgcggcgatt gagcccaaag acggtttgtg aatacgcaaa ggtatcctgt atcgcgcgaa
      601 taatgcgcgg cacttcgtcg gccatagatt tatctttaat atcctgtaga aaagcctgtt
      661 tgagaacgcc gtacgaacat tgcaaacgcg ccatctcgct catcaaaaga cgactgggaa
      721 tcggtgtgcc gcattcgggt aatgtaagtt caccgaaacc aaccggcatt ttgaaaccgc
      781 tacgtacccg ttcgatcgtg ccgtccacga tcggttcgta gatatgcgta ttttttccgg
      841 tacgacggtt ctcttccgcc aaatctttaa gaccaataaa tacgcggctc agcggataac
      901 gcgccaactg ccgtgcacta cgcaaagcgg cttgcgtttc gataacgata cccacaccca
      961 cgcgcccgtt agcccactgc agtgtttttt cgatttgact gctttcggtt accatgggta
     1021 gtaaaatctc cgtcgcgccg cctttgatcg cctcctcgac ctcgcgttcc gtcccggggc
     1081 taaaacggtt gatgatgcag aggatcggcg ccgaggtgat agctcttatt ttagttaaat
     1141 cgtcaatgct atcatgcgtg acagtaccat ccgtagagct tttaccgata tgctcccaat
     1201 tgataccgat acctttcacg ccggcttgca gcgctgattt tacagtttct tcatgcgtag
     1261 aaaaaagata caaatcaaac gtcggttccg tcgccatgca cactcccgtt acgcgaactt
     1321 atttcgaata gttttgataa gctttatgtt tatgatagta ataagatcat tacagataag
     1381 tatagcgaat atcgtataaa tataaaaaaa ccgcaagcat cgtttattta acgcctttaa
     1441 cgataaaata ggatgcgagt cgcacgccct gatgatgccc cagccaacga ataccaatat
     1501 ctaataatcg cgctatcgtg cgacgtcctg tcggatgcgg atgaccataa aaaccggagt
     1561 aaaccgtcgt ttgaaatccc gcttctttca aggcttgagc atatacctgc ggttccaaaa
     1621 gtcggtctgc ccagtttcca ttgcccggat cgcacgtatt ggtaggatgg tccggtttag
     1681 gcggcatacg gccttctgtt ttaaaaattt tcaccgttgc gcggatgtcc tctgctttca
     1741 taccgcgtgt acgctcagct aattcatata tcatttcttt ctccaaatcc gcattttcag
     1801 cgctgatcat ttcggctctg atgcgacgat aggaacgtaa atcatcccga tctttgtggc
     1861 cccactcggc tttgcgatct tcgagttcga cacgacgctg actggcttgc agtgaacggc
     1921 gaatagccgg attgagggga ttggcatgcg ttgacatgac caatgacatc gctttggaag
     1981 gcaactccgc ggcttgtttg aaaaatagtt catgccgata aatgtgttcc aacacatcgc
     2041 atgatacaaa tgcatcgcaa tttatttttt cgcgctcaag aaactgatga accgtatcca
     2101 gatcgccgca cacaaaatca ttgatcggga ttcgccaaat ctccgaaagt tttcgtacgt
     2161 cttgtacgga tgtttcaaaa atatcattat agacgacgcg ccccacgccg gctgctttgg
     2221 ctatgcacga aagcaaaccg catcccccgc cgaaatcaac caatgtacaa cgtgcgagat
     2281 cgttctgttt ctccaatgcg agtgcaagta tatacacata tttctgaaac gtcggcgtca
     2341 attttgcagt atattttttt agataccttt tattgtagtc cgacacgggc acggccgacc
     2401 aatccgtcgt tttcagtcgc tcggctaccc tttccgcgcc gcggttgagt tgcgtcatta
     2461 ggtcggcgtt attcttaacc ggcaatcccg gaaaaacaaa tcgcatattt cagatattcc
     2521 ttttttcaaa aatcgtcaat ggtaattaat gtcgcagaaa atggtgtgat tcgcaagaag
     2581 gtatttcact acgtcgttat gaatgatgga tgcaaattat atttgtaagt atggctgttt
     2641 ttgataaatt gcgcgcgcat aaacggagca attaactata tgcatgcgac gacggacgat
     2701 cgaaaatcct acaaccgcca aaaactgtgg ctcagcctac tatccggtgt attgagtttt
     2761 tgtgtattaa tcgcgctttt tttttccggt ttgtcagata cgttggcccg atgggttatg
     2821 tcgcaaacgg aacgacctta tttacaattt ctactgtatc tatttgcttt gggtttattg
     2881 caaagcatcg tcacttttcc tttgagtttt tataacggat atattttaga gcaccgatac
     2941 cgtttatcca atcagtcatt caaacaatgg tgttttgaaa atgtaaaagg cttggcgctg
     3001 ggcatcgtga tcggcgtccc tttactttta attttttttt acacgctccg caactttgga
     3061 ttttggtggt ggctgccttt ggccggcata ttgtttttat tctccgtttt gttagcacgg
     3121 atcgccccga cgttgatttt tcccttgttc tatacgttta aaccggtcga aaaaacagcg
     3181 ctcgtcgagc gcatcacccg cttgtgctta aaagccggtt tgcaagtcca gggggtattc
     3241 gaatttaatc ttagtaaaac taccaaaaaa gccaacgcgg cgtttgccgg tatcggtaaa
     3301 tccaaacgta ttttgctggg cgatacgctg ttacaacaat tttctgacga tgaaatcgaa
     3361 gttgttttcg ctcacgaagc cggtcactat gcgcacagcc acattaccaa aggcatgatc
     3421 tggggcatcg tttctacatt cggcggactc tgtttgacag ctttcatata tgataattta
     3481 tttcctgctt ttgaatatac ggatcgtgcg gaattaggcg cattgcctct cattgccatt
     3541 ctgctgagtt tatacagtgt tatcactaca ccggccggac atattctcag tcgtcggcat
     3601 gaacgcgaag cggacgcgta tgcgctgcgt gaaaccaaaa accgtgcggc ttttatttca
     3661 gccatgcagc gtttgtcgga taccaatctt gcggatgaga atccgcatcc gctggtggaa
     3721 tggtattttt attcacatcc cgcaatcggc aaacgcattg ccgccgcgga aacgatgata
     3781 ttggaatagc atctatgtta cagcttgtca atcttacggt cgaatttggc ggacgcacgt
     3841 tattttctaa cctcacatgg aagatcaatg atcgcgaccg cgtggctttg atcggtccca
     3901 atggggccgg aaaatccaca cttctcaaaa tcatcgcgcg tcagcaatcg gcaacaagcg
     3961 gcaccatcgc ccaacccaaa catttgcgcg tcggatattt gccccaggac ggattgcatg
     4021 cccacggtaa gccgcttctc gaagaagtcg tatcggcatt tgatgaaatc gttcggatca
     4081 aacaaagact cgacgaattg gaacatgcat tgcacactct ggatcacgag gcaccggcct
     4141 atgccagagc attggacgaa tacgggcatc tgcatcagcg cttagatgat atgggcgcgg
     4201 cctccgccga atctaaagcc gccaaaattc tgaacggtct cggttttaaa gaaagcgaat
     4261 ttgataaact gtgcgaactt tttagcggcg gctggcaaat gcgtatcgcc ttagctaagc
     4321 ttcttttgca aaatcccgat gtattattgc ttgacgaacc aacaaaccat ttggacattg
     4381 actccatcga gtggctggag gaatacatca aaaattacga aggcgccatt tttatggtgt
     4441 cgcatgatcg ttattttata gacaatgttt gcaaccgtat cgtcgaactc gaacgccaaa
     4501 cgttgaccga ttatgtaggt tcttacgacg actatgaaga acagaaagca ttggctgaag
     4561 aacaacttct cgcggcgtac gaacgccagc aggatgaaat aaaacgtgtt cagcttttta
     4621 ttgatcgctt ccgttataaa gcgaccaaag cccgccaggc gcagagtcgc gtcaagatgc
     4681 tggaacgcat ggaccgcatt caattgcctg aggaagaaaa acgggccatc cagtttcggt
     4741 ttcctcaacc gatcaaaagc ggacgggtcg tgcttgagat cgctggaatt caaaaatcgt
     4801 acgacggacg accgattctc aaaggtatcg aatttgctat tgagcgcggc gaaaaagtag
     4861 cccttgtcgg tcctaacggc gccggtaaat caacgctgtt gcgtatcatc gcaggcgatg
     4921 aaacaccgga taccggcacg atcaaacacg gttacaatgt tacgatggaa tattttgcgc
     4981 agcagcagtc cgataaactg gatatgacgc gcagcgtgta tgaagaaatc gcttcggcgg
     5041 caacgaatca gccgccgcta ttactgcgta ccatcttagg tgccttcctt ttttccggcg
     5101 atgatatcaa taaaaaagtc aaagtactca gcggtgggga acgctcgcgt ctcgcctttg
     5161 ccaaaatgct actcaatccg gcaaatttta tcattttgga tgaaccgacc aatcacattg
     5221 acgcacaatc caaagaaatt ctacagggtg cgctcactga ttatgaaggc acattgctca
     5281 tcgtctccca cgatcgctat tttttagaaa gccttgtcac aaaggtcata gaggtgcgcg
     5341 aagggcatgc taaaatttac cccggtacat ttgctgaata tcatgagcgc aaaatcaaag
     5401 agcacgccga agccgctgca cgcgaatcta tcgctgaagt aaaagtaaaa ccgattgcct
     5461 caaagcccga acgtgaggtt aaaaaagaac cggtcaaagc tgaccaacaa gccaagcgta
     5521 aacgcgaaaa acaactgagc caaatcgaaa gcgatattca aaaagccgaa acccaaaaaa
     5581 tagaaatcga agctctgttg accgatcccc tgctatataa aaacccggat cgtcaaaaaa
     5641 cggtccaatc cgaatacaat gctttgcatg cacgtatcac ggcgttgtat gaagagtgga
     5701 atgccctcgc tgccgagctg tgattgtttt cggtctgaaa actttttaaa agtcaaagcc
     5761 ccgtaataca tggtattatc ggggctttac tatgaggagg agaggaaaac catacgtttg
     5821 accgtacagt actctcccaa tacgtaatct caaaaaaaaa gttgcgatca ggataaaacg
     5881 ggcattaacc caaggtggga cgacctacgg taagaacaaa tacttttgaa gcaccggctt
     5941 tttttaaagc cgcagcgcaa gcatttgccg tggatcctgt cgtaataata tcatccacga
     6001 ggatcacgga ttgatcttgt acgctcgcat cggccatcgc tataaaaacc ccgtctacat
     6061 ttttgatacg atcttccgcc gtattcattt tcgtttgtga ctcggtgtgc cgaacgcgtt
     6121 gcaaagtttc tacataggat aaaccggcca attgccccac ggcacgggct agtaattcgg
     6181 cctgattgta tccgcgttca cgtttacgga tggggttcaa aggtatggcc gtaatccgtg
     6241 tggctgtttt taacatcggg ttagtatgca ctatctgcgc caatcggtca gcaaattttg
     6301 gaatcagcga taacgcacga tcatatttca aagcatggat agatttctgc aatacctcat
     6361 cgtagtaata tcccgcatgg atcacatcga tcgcttcgca tcggattatt ccttcgtaat
     6421 cgctttcgcc gaccgttttg agagcgttcc agcatgactt acaaatatgc ttgtcggcag
     6481 gatccaaacg tactcggcaa gccaagcaag ttgacggata aagaaaatcc gccaaccaat
     6541 ataagccaag acgtaacgag gctaacatca tacacctgta agtaagtcgt taagacgtgc
     6601 agaaaattgg gagcgcggca ttaccgtatc gcaaccggct tcggacgcct tatgtgccaa
     6661 ttcagtttgc acatgcgaaa aaaaagcaat gataggaata gacatagcca ccgaatccgc
     6721 cttgagtaaa cggatcaggg cgaacgggtc tcttttttgg atattgagat ctacaaaaat
     6781 tttacgatag ctctgcgcca aaaataactc tcggacattt tcattcgcct tatctgcaac
     6841 gaccgtttga atgcctagcg cacgggcttg cgtttttatt ttggacaaaa aaaggatgtc
     6901 ttcgatgaaa agcaaaatca tgcggatggc gcggatgaag gaatgaaaaa cttgaaagtg
     6961 ctgcctttac ctaattcgct ttcggcccat attttaccgc cgtgcgcttc aacggcgagt
     7021 ttacaaaatg tcaatcctaa gccgcgatcc gatttgagtc cgcgttgttt gagatcaact
     7081 tgcgaaaatt tttcaaaaat cttctcaaga tattcgacag gaatcccttc gccggtatcc
     7141 gtcaccgcaa aacacatgcg atgctctgcg gcttgccatt cgcacgtcag cgtcagtttc
     7201 ccgcctttct gactgtgttt gatggcatta aaaaggaggt tagcgatgat gcgcatcagt
     7261 aaatcacgat ccgcccacac catcgcgtta tcgtcactca agtgcatatc cataaccaac
     7321 tggtattttt cgatcatata ttccagttcg ccgatattgt gcataaacac ttctttgacg
     7381 gacacggctt ccggacgaat caccagttta ttctcttcca tccggctcac atccaaaagt
     7441 tccgtcacca ttttgagcaa cgtaccggta ttatcttcca gttttttgag aatagaaatt
     7501 tgcttttcgg tcatgttctc gctcagcatc gtgagcaatt cggtacatcc cataatcccg
     7561 gttagcgggt ttttgagatc atgaacgagc atttgcgtga gcgattcgcg caagtgctgc
     7621 agttgcagaa gctggtcgtg tttttcttca agtgcatctt gattggtacg cagttcgtca
     7681 tgcaagtctt tgatacgcat cagggaacgg atgcgcgaca tgagctcggt gtagttgatc
     7741 ggtttgggca aaaaatcatc ggcgccggcg tccagtgcgt gcgcttcatc ctccgggcgc
     7801 tgcaaggccg taaccatgac caccggcagt aatgccgttt ccggattggc ccgcaagcgg
     7861 cgacagactt caaatccgtc catttgcggc atcatggcat caagcaaaac aagatccgga
     7921 gattctgacg cgacaagagc caacgcctct tcgccggagt gcgctgtaat cgctgcaaag
     7981 cctttacgaa tgagaaattg acttaggaac cgaacgttta attcctcatc gtcaacgact
     8041 aatatttggt gatgttgttt tttcatgaga ccggacacaa gtgtactaag ttcgtacacg
     8101 ataatcaaat aaatttacgt aagcccaaat cgtcttgcgc ctatgtcgta ttccgtttat
     8161 attgcgcgca cgtatttgat ttcttcatac accagttttt ttgtttgata caccacccta
     8221 tgcgccgatt attttattca gcattttatt tttgtgtcat cgttcttttt tttgttcttt
     8281 tactggataa atggttgatg cccatgtacg tggatttggg caaagatatt gccgtaccgg
     8341 ccgtcacagg gctatcttcg gaaatggcac aaaaaaaact tagtgaatta gggttttcgg
     8401 tggtactaaa gcgccgttat gatatctcgg tagcccctaa gacggtgata caacaaagcc
     8461 cggaaccgca agcgttggta aaagcaggtc gtcagattca tttggttatc gcagatgata
     8521 atcaaacggt catcatgcct tcgctgactt tatccacata ccgtgatgct gtttttaatt
     8581 tacaatctat cggtcttgcc attggcgtac tcgattcggc ttcgtccaat gaatttcctc
     8641 agggagttat actcaaacaa tcgattgaac ccaataccgt cgttcgcctc gggcaatctg
     8701 tcgaccttac aataagcctc ggaaataata taaccgaatc caaggtacct tttttgatca
     8761 acctcagtct caaagatgcc aaagctcgga tatccgattc cggtttgcga ctcggaaaaa
     8821 tcgaacgtaa atttttgccc gatttgatac ctgatacggt cgtcggtcaa tcgctggatt
     8881 cgtcgctcgt cgtgactccg ctgacaatca ttgatcttac cgtgagttca accgatagtg
     8941 aacaataaat tcccaaaaaa tctccgaaaa ttttgtttcg taactgatgg atttattgcc
     9001 gatccggcgg caactattac cggacccatg gttcaaactt atatcatagg caaggagctt
     9061 tcaaatcgaa attgggaagt acactacgta gcctataata agtccggcat gcgtggctac
     9121 atgaggcatg aaggattaca tgtccattgg ataaaagcac gccgctattt acccatactc
     9181 agtttaccgt ccgtatggat ggcattaatt cgcatcaatc cggacgtact gtatcaacga
     9241 gggcgcgaca tattgaccgg ccttatggct ttgtacggcc tgatgtacgg taaacacaca
     9301 ctctgggcaa gtgccggcga aacgggtgtt gagcgtaaaa aatatgcgtc ccaaaaaaat
     9361 cgtcaaaagg gaaattttat tcgtcgttgg gtattgcaaa tcgaagcact gatcaacgat
     9421 acgatctgta actttggtat gcgccgcact gaccagatga tcgtacaaac agcctatcaa
     9481 caacaacgtc tgcttgttac ttatgcccgt tcctcgacga tcatcaaatc cggccatccc
     9541 ttgcctccat ctgttcaacg tgccttgcct ataaaagtac tgtggattag cagcatcaaa
     9601 ccggtcaaac acgttgattt gtttttagat ctggctgata tgtgtaaaaa tgagcccatc
     9661 gaattttgga ttgccgggca gattgtgcat gaagaaacag gtcataaaat gcaagagcgc
     9721 attgcaaaga tgaacaacat tcgctatctt ggcgctgttc cgtttgaaca aagcagcgca
     9781 cttatcgcgt catcgcatat actggtcaat acgactgagg tcggttatga aggattaccg
     9841 aacgcttttg tgcaagcttg gcttgccgga actgtaacat tgagcctgca taccgatccg
     9901 gatgccgtga tcatgaaaaa tgatttaggc tacgtcaatc cggaattggt acaattaaaa
     9961 gaaaagatgc tccggttcat tattgaacat gcggtgtggc tccgccaaag taatgcatgc
    10021 cgccaatacg ccgtagaaaa ctattcagtg gtttccatcg tcaatcaaat tgagaaaact
    10081 ttataattaa agaaaaattt atgtctagta ttagcgaaca aaaacatcaa cgggttgtcg
    10141 aattagaaaa tgaattgcgc tctgaaaatt tcaaacgacc acagtatcga tatggtatgg
    10201 atctggtgaa agccacggga ctgactaaag gtcgtatcat tgagtttggc ggcggtcgtg
    10261 gtgaattcag ccgactactt aaagatcacg gttttgaggt tgtttttacc gatatcaatc
    10321 aggcaaatgt cgaatttgca aaatccatag gatttgaagg atatgtcatt gacgccaaca
    10381 gcggcatggc ggatcaaaaa gccgattcgt acgacggagc cgtcatgttg gaagtcatcg
    10441 aacacgttac ccaagccgaa ttgttactta cggaaacaca ccgcattttg aaacccggcg
    10501 ggtttctcgt cttaagtaca cccaatccgt acttcctatg gcgtcgtctc tctgtgcttt
    10561 tcggtaagcc catagacggc gaaggctacc actggcgcta ttttacagtg tattcactgg
    10621 aatccactct tcgcgaaagc ggatttacca ttattacgca taatcactat gcctccacgt
    10681 tcggcttagg taaaattctg aagcttatcg gctatacgaa gacatttcgt tggccaagat
    10741 ttttgcacgg tctgctggta agaaaaatat attttctagc aaagaaataa aaagttttac
    10801 attattaatc acagtgaata atacgatttt aaaagaatgg acagtttata atgaaaggca
    10861 tgattctcgc tggcggcgcc ggaagccgct tatatcctat aagcaaggtc tatagtaaac
    10921 aactgatgta tgtttacaat aaacctatga tttattaccc cctatcaaca ctgattttgg
    10981 gcggcatcaa agatattcta atcatcacga cacctgatga cgcacctatg tttaaaaaac
    11041 tacttggtga tggaaaacat atagggctta atctgcaata tgccgaacaa ccgaaaccgg
    11101 agggtttggc gcaggcgttt ataataggtg aaagtttcat caacaacgag gctgtttctc
    11161 ttattttagg agataacttg ttttacggat atttggattt ttttaaacga gctatacaaa
    11221 acaccaaagt cggtgccacc atttttggct atg
//
LOCUS       DAOQNQ010000078        11240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2058_length_11240_cov_6.688422, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000078 DAOQNQ010000000
VERSION     DAOQNQ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11240
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2058_length_11240_cov_6.688422"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PK754_06690"
     CDS             complement(<1..193)
                     /locus_tag="PK754_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09226.1"
                     /translation="MKKIGILFGMENTFPQAFVDRVNSKKEKGITAELVSIDKVIQGE
                     PCGYDVIIDRISQDIPFYRG"
     gene            complement(292..1476)
                     /locus_tag="PK754_06695"
     CDS             complement(292..1476)
                     /locus_tag="PK754_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNB09227.1"
                     /translation="MNEHTQTHTYIAKTFFGLEPVLSDELKNIGATQIKMLNRAVSFS
                     ASEEIMYKANLWLRTALRIIKPIHEFKAINEEQLYRHTQKMDWSQFLEPKDTFVIDST
                     AHSKFLTHSHYAALKVKDAIVDQLRDQFGERPSIDAENPTLRINLHIAHDDVTIGLDA
                     SGNSLHKRGYRTGTNEAPLNEILAAGMILLSGWDRRAPLLDPMCGSGTIPIEAAMIAL
                     NVPPGHLRTSFGFFTWKDFDPALWKKVRTDAETQRVRNSDVRIFGSDMDRGILDKTRQ
                     NIINAKMDAHISIASKSFSDVTPPATPGMIITNPPYGERLKPADLQTLYKMIGDVLKK
                     NFTGWDAWVISGNKDALKQLGLHPSKKLTLYNGALECRFQKFAMYRGSIKQKHQIPND
                     EK"
     gene            complement(1490..1843)
                     /locus_tag="PK754_06700"
     CDS             complement(1490..1843)
                     /locus_tag="PK754_06700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03067 domain-containing protein"
                     /protein_id="HNB09228.1"
                     /translation="MQLDGTWQMVSAELAGGAFEDEDVKDMRLIIKGNAYESHVGKDC
                     DKGVLRIFHYDRPMGLDVIGTDGPNAGRTIFAIFEVSKESLTICYQLEGNARPLHFET
                     KPGTMLFLVRYKRTA"
     gene            complement(1961..3217)
                     /locus_tag="PK754_06705"
     CDS             complement(1961..3217)
                     /locus_tag="PK754_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09229.1"
                     /translation="MYLHRIFFVAAFLLFAVFGCYRDPGDGSPQLPPSGPRWIEANLW
                     RMALQPDGRWGYDVTDDDHDGNSSGAEFPKNSGNYGLYAGGLMVGAYKDGAPVVLDMN
                     WTTEFTGGRITNTTPTSVDQLEYSSEDNARTYFIGSTSHHDSPDLQSWPSDYGGPWDS
                     LLSKPILISDLDTWASGHDLRADSLSEEAGQSSLGIEVRRMTYSKTGASWENATLIRF
                     QVVNKSDQNYANAYLSWWVDHNLGSTVTNDAVGTDTARNLVYTYNLSPDVTSTGKQYA
                     VGHTLLYFSSSGINSKWNSTYAYYNGNDPVGQTEKYRIQQGGINHKTGSSLDSTFAGS
                     VGGKYVYPGDPVTGTGTLCTQGRNGRLIGNVGPFTLESGKVYDIVVAVIGGEGTDRLN
                     AISDLRTKSDFIRSAFTTISPNMKLK"
     gene            complement(3308..4474)
                     /locus_tag="PK754_06710"
     CDS             complement(3308..4474)
                     /locus_tag="PK754_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB09230.1"
                     /translation="MVPIKYIAGGFKRRALTTFITIAGIALVVFVYTAVLMMSYGVEK
                     TLASGGSERNAVIARKSSQGEISSILDGETINVIMTLPQIAKGADGKPLASAEPVVVI
                     NLDRVGGGISNITVRGVEPTAITLRPNIRITEGRMFNTGSREVIVGTSVTERFIGARM
                     GDKIKFANDYWTIVGVMDAKGSAFESEVWGDARQLQAAFNRGNSASTMNVRMADGVTI
                     EEFKQAFENDKRLSQFEPKTEVKFYKEQSEALATFIAVLGTAITIIFSFGAVIGAIIT
                     MYTAVANRITEIGTLRALGFRRRSVLGSFLLETIFISLSGGVIGVIIASFLQFFTIST
                     MNFNSFSELAFSFSLSPGIIINALIFSTIMGLIGGFFPSIRAARLKIVDALRNA"
     gene            complement(4512..5669)
                     /locus_tag="PK754_06715"
     CDS             complement(4512..5669)
                     /locus_tag="PK754_06715"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB09231.1"
                     /translation="MLFKMMLRNALRHKLRTILTILGIAIAVGAFGLLRTVITSWYAA
                     VEAAAVDRLITRQAVSFIFPLPYAYRDQILKVPGVEKVGYFNWFQGVYKDKSEFFPRM
                     ACDPETIFDVYPEFLVTPEEKAAFQKERNACVVGADIAKKHNLKIGDVMSIEGDIYPG
                     RWEFVVRAIYQPKTPAIDATQMFFHWTYLDERMKQDMPGREGNVGWYAVKIKDPSESA
                     AVSEAIDAIFKNSNAETKTETERAFNQGFIGAYGAIINGINIMAFMIIGIILLVLANT
                     MIMSARERNREYAVMKTLGFTGKHIFGVVTGESIIIGILGGIAGYAVTLFFVGAFSVA
                     VPKNFFPVFILADITVIQEFSFAVIVGILAGIIPVVQAIKTDIVDGLRYVG"
     gene            complement(5709..6380)
                     /locus_tag="PK754_06720"
     CDS             complement(5709..6380)
                     /locus_tag="PK754_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB09232.1"
                     /translation="MPGLVEIQNVSKAYQRGSMSIPVLQNINLSIADGDFMALMGPSG
                     SGKSTLLNLIAGIDKPTQGSIIVGGTDVASLSESALAKWRSYHVGFIFQFYNLMPVLT
                     AYENVELPLHLTNLSKKERDERIQKVLTIVGLGDRMKHYPNQLSGGQQQRVAIARAIV
                     TDPTLIVADEPTGDLDRTSAEEIMTLMTRLNQELGKTIVMVTHDPHAAEKAKTQRHLD
                     KGTLS"
     gene            complement(6415..7665)
                     /locus_tag="PK754_06725"
     CDS             complement(6415..7665)
                     /locus_tag="PK754_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB09233.1"
                     /translation="MASEAEKVDLSSLRIQREPMKFDPEDKRSSGNLVKWALMVIGVV
                     AIGFIGYMSRNMFKSTPVVQLTTVAFVAQGSGGGVLTASGYIVPQRKASVASKGTGRL
                     VFLGVEEGDRVQSGQVIARLENNDIQAGLEQARANLLVGQAQVEQAEADLERVKRNYE
                     RSETLLKSNVITDADFEIVRAEYRSAQARVTSAKASVAALQAGIHATEVELENTIIRA
                     PFDATVLTKNADVGEIVAPFAAGANSRGNVVTLADMSSLMLEADVSESNIERVKIKQP
                     CEITLDALPEKRYRGEVWKVVPTADRSKATILTKIRFIDIDNRVLPEMSAKVNFLTEA
                     ISDSAMQAKPKLMVNTSAVVERNGRKVALSVVSGKIRAIPVMTGERNGNKTEIVNGLT
                     AGDKVIDNPAADLNDGDAVTTEQK"
     gene            complement(7769..8503)
                     /locus_tag="PK754_06730"
     CDS             complement(7769..8503)
                     /locus_tag="PK754_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNB09234.1"
                     /translation="MSTPRLRVIIVDDEALARQLIREYLASHTDISVIEECANGFEAV
                     KAITEINPDLVFLDIQMPKLSGFEVLELTQRKHGVVFTTAYDQYALKAFEVHALDYLL
                     KPFSRERFDAALAHALQHIRDAITWEAPSSLTSDKKTERILIREGVQVHVIPVTQIDY
                     IEAQDDYIAIYATGKCYLKTQTLSDIEKQLDSSRFVRIHRSYIVHVERIARIELYAKD
                     SRTAILRDGKQIPISRSGYDHIKQLL"
     gene            complement(8500..9561)
                     /locus_tag="PK754_06735"
     CDS             complement(8500..9561)
                     /locus_tag="PK754_06735"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNB09235.1"
                     /translation="MHPVLGNIRSLAWYILVWLFAGALIAMLIYFTGRGDGTQAFAFA
                     IPLTLIYGFINLSAYYVSRSSPLVSDRRLRVVLTALFNAMLTAFFWLALAMGWNVILH
                     EWQTAIVMDTGTTMIMIVVGTVLYLLSIAIYYVMMMARQAEQAKQNELQSAVLSKDAE
                     LRMLRAQIDPHFLFNSLNSISALTHSDPDGARCMTVELAAFFRQTLALAQKPLITLRE
                     EWNLCEHFLSVEKVRFGNRLTVEMMLPEQLNDILLPPMLIQPLAENAVKHGIAQCVEG
                     GTIRIHALTDGPYVHIVIENPMEDESSYSDGTHIGLDNIRKRIQNQYGRDARLTTVKQ
                     NRLFVAKLSIPVSPGEASA"
     gene            complement(9561..10256)
                     /locus_tag="PK754_06740"
     CDS             complement(9561..10256)
                     /locus_tag="PK754_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HNB09236.1"
                     /translation="MKTDSKIPGQVILGVIAIVLGVLFLLDNLRIIEDFRLSRLWPVI
                     FIIFGVLKIRQSRTVPSYFVGSVLMCVGTIMILHRLGIIYFSWHTWWPVFMILAGGAF
                     ILKGFYRHRGQDETNIPKADNEHVVQATAIFGGNSRIIASQDFKGGEVTAVMGGCEID
                     LRHASVADDATIHVFALFGGIDIKVPTDWAVFLNGTPILGGFDDKTLHPADQTKRLHI
                     QGYAIMGGVEVRN"
     gene            complement(10240..10623)
                     /locus_tag="PK754_06745"
     CDS             complement(10240..10623)
                     /locus_tag="PK754_06745"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HNB09237.1"
                     /translation="MFTHHSHDIKRHLIWGVALIAIGFVVLLDRLHVYDIGPLWKFWP
                     VFLIVGGISDLLSYNKARRIADGVFWILTGGWLLAVQFYAWGLTYRNSWPFLLIAWGV
                     SKITRSLLNKYYFTPQTTTHYENGL"
     gene            complement(10829..>11240)
                     /locus_tag="PK754_06750"
     CDS             complement(10829..>11240)
                     /locus_tag="PK754_06750"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNB09238.1"
                     /translation="AGASRGVMASLVNAYAEEEAPTGDGKTETRVVMKFHPTLAPVKA
                     AILPLVKKDGMPEVSMTLYNELKKHYKIFYDESATVGKRYRRQDEIGTPYCFTIDSET
                     LQNQTVTVRHRDSMRQERIAMDQVLRFLQNNLNA"
BASE COUNT         2909 a         2618 c         2707 g         3006 t
ORIGIN      
        1 atccgcgata aaaaggaata tcttgtgaaa tacgatctat gattacgtca tatccgcacg
       61 gttcgccctg aatgacttta tcaatcgaaa caagttcggc cgtgatgcct ttttcctttt
      121 tggaattaac gcgatccacg aaggcttgtg gaaacgtgtt ttccataccg aaaagaatgc
      181 cgatcttttt catgataagg ctcccatgat tgaaaaaatt tttaataagt tgaaaagctt
      241 catgatcgaa tagtggggaa atttatatca aacccggtgt gttaaacaag gttatttttc
      301 gtcgtttggt atctgatgtt tttgtttaat gctgccgcgg tacatagcaa atttctgaaa
      361 gcggcattct aaagcgccat tgtataaggt aagttttttg gaaggatgca atccgagttg
      421 tttaagcgca tccttattgc cgctgatgac ccaggcatcc caaccggtaa aattcttttt
      481 cagtacgtcg ccgatcattt tataaagcgt ttgcaaatcg gcaggcttaa ggcgttcccc
      541 gtaaggagga ttggtgatga tcatacccgg tgtggcgggc ggtgttacat ccgaaaagga
      601 tttggacgct atggaaatgt gcgcatccat tttcgcgttg atgatattct gtcttgtttt
      661 atccaaaata ccgcgatcca tatctgaacc gaaaatacgt acatcggaat ttctgactcg
      721 ctgcgtttcg gcatcggttc gaactttttt ccataatgcc ggatcaaaat ctttccatgt
      781 gaaaaaacca aacgaagtac gcagatgtcc cggcggcaca ttgagcgcaa tcatagcggc
      841 ttcgattggg atcgtacctg atccgcacat cgggtcaagc aatggcgctc ggcgatccca
      901 tccagataaa agtatcattc ctgcggctag aatttcattg agaggagctt cattagttcc
      961 ggtgcggtaa ccgcgtttgt gaagcgaatt gcccgaagca tctaacccga tagtaacatc
     1021 atcgtgagcg atgtggagat tgatgcgcag tgtggggttc tccgcgtcaa tagacgggcg
     1081 ttcaccgaat tgatcgcgca actgatctac gatggcgtct ttgactttca aagcggcata
     1141 atgcgaatgc gtcaaaaatt tggaatgggc cgtactatca atgacgaagg tgtctttggg
     1201 ttcgaggaac tgcgaccaat ccattttttg cgtatggcgg taaagctgtt cctcgttgat
     1261 cgctttaaac tcgtgaatcg gtttgatgat acgaagcgcg gtgcgcagcc atagattggc
     1321 tttgtacatt atttcttcgc tggcgctaaa ggatacggcg cggtttaaca ttttgatttg
     1381 cgtggcgccg atgtttttta attcgtccga taaaacaggt tcaaggccga aaaaagtttt
     1441 agcgatgtag gtgtgtgtct gggtgtgttc gttcattagg ggacgcgttt caggcggttc
     1501 gtttgtagcg cactaaaaat aacatggtgc cgggtttggt ttcaaaatgc aaagggcgcg
     1561 cgttgccctc aagttggtaa catatcgtga gagattcttt tgatacttca aatatagcga
     1621 aaatggtgcg accggcattg ggcccgtcgg tgccgatgac atccagtccc atcggtcggt
     1681 cataatgaaa aatacgcagc acgcctttat cgcaatcttt tccgacatgc gattcataag
     1741 cattaccttt gatgataagt cgcatatcct tgacatcttc gtcttcaaag gcgccgccgg
     1801 ctaattccgc agaaaccatc tgccatgtac catccagttg cataaaaaat tatccctgat
     1861 tatcgctttg atggaacaaa catcactatt tgttcggcga taatcaagga tattagttgc
     1921 tataaaggtt tttgacgaaa attttaagac taaatcaaca tcacttcagt ttcatattgg
     1981 gagatatagt cgtaaatgca cttcggataa aatcggattt ggttcttaaa tctgaaatgg
     2041 cattgagacg atcggtacct tcgccgccga ttacggcaac tacgatatca taaaccttgc
     2101 ctgactcaag tgtaaaaggt ccgacgttac ctataaggcg tccgttgcgc ccttgtgtgc
     2161 acaaagtgcc ggtgcccgta acaggatcgc cggggtagac atattttcct ccgacacttc
     2221 cggcaaaagt actgtccaaa gaagaaccgg ttttatggtt gatcccgcct tgttgtattc
     2281 ggtatttttc ggtttgtccg acggggtcat ttccgttata gtaggcgtat gtggaattcc
     2341 attttgaatt tattccgctc gaactaaagt acaaaagcgt gtgtccgacg gcatactgtt
     2401 taccggtgga agtgacatcg ggggagagat tgtatgtata aactaaattg cgcgctgtat
     2461 ctgtaccgac ggcatcattg gttaccgttg aacccagatt atgatcaacc caccaggaaa
     2521 ggtaagcatt ggcgtaattt tgatcggatt tattgactac ttgaaaacga atcagtgttg
     2581 cattttccca tgaagcgcct gttttggaat aagtcatgcg ccgcacttca atccctaagg
     2641 aagattgtcc tgcctcttcg cttagggagt cggcgcgaag atcatgtccc gaagcccacg
     2701 tgtctaaatc agatattaga atcggttttg aaagtaaact gtcccatggt ccgccataat
     2761 ctgaaggcca actttgtaaa tctggagaat catggtgcga ggtggaacca atgaaataag
     2821 tacgagcatt gtcttcggat gaatattcca gttgatctac cgacgttggc gtagtattgg
     2881 taattctgcc accggtaaat tcggttgtcc aattcatatc caaaaccacc ggtgctccgt
     2941 ctttgtatgc gccgaccatc aatccgcccg catagaggcc gtagtttcct gagttttttg
     3001 gaaattcggc gccactacta ttaccgtcgt gatcatcatc cgtgacatca taaccccacc
     3061 gaccgtccgg ttgaagcgcc atgcgccaaa gattagcttc gatccatcgc ggccccgatg
     3121 gaggaagttg cggtgacccg tccccaggat cacgatagca gccaaaaact gcaaaaagta
     3181 aaaaagccgc cacaaaaaag atacgatgaa gatacataac aaactccttt ctagttgtta
     3241 gtatactgtt gttgtatctt catagttcaa aaaacatggt aactcttaca aaatattttc
     3301 aaaaaaatta tgcgttacgc aatgcatcca cgattttgag gcgtgccgcg cggatggacg
     3361 ggaagaaacc accgatcaaa cccatgatcg tcgaaaaaat caaggcattg atgatgatgc
     3421 ccggcgataa cgaaaacgaa aaagctaact cagaaaatga attaaaattc atcgttgaga
     3481 tcgtgaaaaa ctgcaaaaaa gaggcgatga tcacaccgat cacaccgccg gacaaagaga
     3541 taaaaatcgt ctcaagcaaa aagcttccaa gaacactgcg acggcgaaat ccgagcgcgc
     3601 gtaatgtacc gatttctgtg attcgattag ccacggccgt gtacatcgta ataatcgctc
     3661 cgattacggc gccgaaactg aaaatgattg tgatggcagt tcctagcacg gcgataaatg
     3721 tggccaacgc ttcggattgt tctttgtaaa attttacttc ggtcttaggt tcaaactgcg
     3781 ataggcgttt atcgttttca aaagcctgtt taaattcttc gattgttact ccgtctgcca
     3841 tacgaacatt catcgtagaa gccgaattac cgcgattgaa tgcggcttgc aattgtcggg
     3901 catcgcccca aacctccgat tcgaaggcgc tgccttttgc atccattaca ccgacaatgg
     3961 tccagtagtc attggcaaac ttgatcttat cacccatacg tgcaccgata aaacgctccg
     4021 ttacggaggt acccacgatc acttcacgcg aacctgtatt aaacatacgg ccttccgtga
     4081 tgcggatatt gggtcgcaaa gttatggctg tcggttcgac tccgcgaacg gttatattgc
     4141 tgataccgcc gccaacgcga tcaagattga tcacgacgac cggttcggcc gaagccaacg
     4201 gtttaccgtc ggcacctttg gcgatttggg ggagcgtcat gatgacattg atcgtttcgc
     4261 catcgagaat acttgagatt tcgccttgcg aggatttgcg tgcgatgacg gcgtttcgtt
     4321 cggaaccgcc ggaagcgagt gttttttcaa caccatatga catcataaga acagccgtat
     4381 aaacaaatac gacgagtgcg ataccggcaa tcgtgatgaa ggttgtcaaa gctctgcgtt
     4441 taaaaccgcc ggcgatatat ttgataggta ccatgatagt ttcctttata ttcaatccac
     4501 aattgctatt gttatccgac gtagcgtaaa ccgtccacga tatccgtttt aatcgcctga
     4561 acaacgggga taatacccgc gaggatacct acgatcactg caaacgagaa ttcctgaatc
     4621 accgtaatat ccgctaaaat aaaaacaggg aagaagtttt ttggaaccgc aacggaaaag
     4681 gcgccgacaa agaacaacgt gaccgcataa ccggctatac cgccgagaat gccgataata
     4741 attgattcgc cggtcacgac gccgaaaatg tgtttgcctg taaatccgag tgttttcatt
     4801 acagcatatt cgcggttacg ttcgcgtgcc gacatgatca tcgtattggc taacaccaaa
     4861 aggatgatcc ctatgatcat gaacgccata atattgatgc cgttgatgat ggcgccgtac
     4921 gcaccgataa aaccttgatt gaaagcacgt tcggtttctg ttttggtttc cgcgttggaa
     4981 tttttgaaaa tcgcatcaat cgcttcggat accgcggccg attcactagg atctttgatt
     5041 ttgacggcgt accacccgac gttgccttcg cgcccaggca tatcttgctt catgcgttcg
     5101 tcgagatatg tccaatggaa aaacatttgt gtggcatcta tcgcgggtgt tttaggttga
     5161 taaatcgctc gtactacaaa ttcccatcgc cccggataaa tatcaccttc gatactcatc
     5221 acatcgccga ttttcagatt atgctttttg gcaatgtcgg cgccgacgac gcacgcgtta
     5281 cgttcttttt gaaatgcggc tttttcttcc ggtgtgacga ggaactcggg atatacatca
     5341 aatatcgttt ccggatcaca ggccatgcgc gggaagaatt ccgatttatc tttgtacacg
     5401 ccttggaacc aattgaaata tccgactttt tccacgccgg gtactttgag tatctgatca
     5461 cgatacgcgt aaggcaaagg aaaaataaac gatacggctt gccgtgtgat aaggcgatcc
     5521 acggcggcgg cttcgacggc ggcataccac gaggtaatga ccgttcgcaa aaggccgaac
     5581 gcgcctacgg cgatggcgat accgagtatc gtcagtatcg tacgcagctt gtggcgcaac
     5641 gcatttcgta acatcatttt gaagagcata tatttatgtc cgttttggat gtcgaaggtt
     5701 taagtgattt aactcaacgt ccctttatcc aggtggcgct gggttttggc tttttcggca
     5761 gcgtgcggat cgtgcgtcac catgacgatg gttttaccca gctcttgatt gaggcgcgtc
     5821 atgagcgtca tgatttcttc ggccgacgtg cgatccagat cgccggtcgg ttcgtccgct
     5881 acgatgagcg taggatcggt gacgatcgcg cgggcaatag ccacacgctg ttgctggccg
     5941 ccggaaagct gattgggata gtgtttcatg cgatcgccta aaccgacgat ggtgagcact
     6001 ttttgaatac gttcatcccg ttctttttta gaaagattgg tcagatgcaa cggcagttcc
     6061 acattttcat aggccgtgag gacaggcata agattgtaaa actgaaaaat gaatcccacg
     6121 tgataggaac gccacttagc taaagccgat tcgctgagcg aagcgacgtc cgtaccgccg
     6181 acgatgatag atccttgcgt cggtttatca ataccggcga tcagattgag cagcgtggat
     6241 ttaccggaac cggaaggtcc catcagcgcc atgaaatcgc cgtcggcaat ggaaagatta
     6301 atgttttgca gcacgggaat actcatgctg ccgcgttggt acgctttgct tacgttttga
     6361 atttctacta atccgggcat aattggttct cgggtttagt gaataatgta tgttttattt
     6421 ttgttctgtc gttacggcgt cgccgtcatt cagatcggcg gcaggattat caatgacttt
     6481 gtcgccggcc gtgagaccgt ttacgatttc ggttttattc ccgttacgct caccggtcat
     6541 tacggggatc gcgcggattt ttccgctgac cacactcaat gcgactttac gaccatttcg
     6601 ttcgacgacg gcggaggtgt tgaccatcag tttaggtttg gcttgcatcg cgctgtcgct
     6661 gatcgcttcg gtgagaaaat tgactttggc actcatctcg ggtaaaacac gattgtcaat
     6721 atcaataaat cgtattttgg tcaaaatcgt agctttggaa cgatcggcgg tcggaacgac
     6781 tttccaaacc tcaccgcggt aacgcttttc cggaagcgcg tccagcgtga tttcgcaagg
     6841 ctgtttgatt tttacgcgct cgatgttgga ttcggaaacg tcggcttcga gcatcagcga
     6901 actcatatca gccagtgtca cgacattgcc gcgggaattg gctccggccg caaacggcgc
     6961 tacgatttca ccgacgtcgg catttttggt aagcaccgtt gcatcaaacg gggcacgaat
     7021 aatcgtattt tccaattcca cttccgtcgc atggatcccg gcttgaagtg cggctacgct
     7081 ggctttggct gaagtcacgc gcgcctgtgc ggatcggtac tcggcgcgaa cgatttcaaa
     7141 atcagcgtcg gtgataacat tgcttttgag taatgtttca cttcgctcgt agttgcgttt
     7201 cacgcgttcc agatcggctt cggcttgttc gacctgcgct tgaccgacga gcaaattggc
     7261 acgggcttgt tcgagtccgg cttggatatc attgttttcc aaacgtgcaa tgacctgacc
     7321 cgattggacg cggtcgcctt cttcgacgcc aagaaatact aaacgaccgg tgcccttgga
     7381 agcgacggac gctttgcgtt gcggcacgat ataaccgctg gccgtcagaa cgccgccacc
     7441 gctgccctga gctacaaagg cgacggtagt caactgcacg accggtgttg atttgaacat
     7501 attgcgcgac atatagccga taaacccgat ggctacgacg ccaatgacca tcagcgccca
     7561 tttgactaaa ttaccggagg agcgtttgtc ttccggatca aatttcattg gctcgcgttg
     7621 aatacggagc gaagataaat ctactttttc tgcttcagat gccataatta tgtttttgta
     7681 tagacggtga tgttttattc aaacgaaacc aaagtcagta aaattactta aaattgcaat
     7741 aattcgattc aaatcatatc aaggcaaatc ataatagttg cttgatatga tcatatccgg
     7801 agcgactgat cggtatttgt ttgccatcgc gtaaaattgc cgttcggctg tctttggcgt
     7861 agagttctat ccgcgcaata cgttcgacat ggacgatgta cgaacggtga atgcgtacaa
     7921 aacgtgacga atcaagttgt ttctctatat cggacaatgt ttgtgtttta agataacact
     7981 tgccggtagc gtaaattgca atgtagtcat cctgcgcctc gatatagtct atttgtgtta
     8041 cgggtatcac atgcacttgc acgccctcgc gaatgagaat gcgctctgtt tttttatccg
     8101 acgtcaatga cgacggagct tcccatgtga tcgcgtctcg gatatgttgc agtgcatgcg
     8161 caagcgcggc atcaaagcgt tcacggctaa acggcttgag taaataatcc aatgcatgca
     8221 cttcaaaagc cttcaatgca tattgatcat acgccgtcgt aaaaaccaca ccgtgctttc
     8281 gttgggtcaa ctctaaaact tcaaatcccg aaagtttagg catttggatg tcgagaaaaa
     8341 ccaaatcggg attgatctcc gtgattgctt tgacggcttc aaacccattg gcgcattctt
     8401 cgatcacgga gatatctgta tgcgaagcga gatattcgcg tatcaactga cgggccaatg
     8461 cttcatcatc tacgatgata acgcgaagtc gtggtgtgct catgctgacg cttctccggg
     8521 cgaaacaggt attgataatt tagcgacgaa taggcggttt tgttttacgg tagtgagccg
     8581 ggcgtcgcgc ccatattggt tttgaatacg tttgcggata ttatccaaac cgatatgtgt
     8641 tccgtcggaa taggatgatt catcctccat tggattttcg ataacgatat ggacatacgg
     8701 tccatcggtt aatgcatgaa tacgaatcgt accgccctca acgcactgtg cgatgccgtg
     8761 tttgacggcg ttttccgcta aaggttgtat cagcattggc ggtaaaagta tgtcgttcaa
     8821 ttgctcgggt aacatcattt ctacagtcaa acgattgccg aatcgaactt tttcaacaga
     8881 caaaaagtgt tcacacaggt tccattcctc gcgcaaagtg atcagcggtt tttgcgcaag
     8941 cgccagggtc tgacgaaaaa aggcggccag ttccaccgtc atgcatcgcg caccgtcagg
     9001 atcgctatgc gtcagagcgc tgatggagtt gaggctgttg aataaaaaat gcggatcaat
     9061 ttgcgcacga agcatccgca actcggcatc ctttgaaagt acagcgcttt gcaattcatt
     9121 ttgtttggct tgttcggcct gccgcgccat catcattaca taataaatcg cgatggagag
     9181 taagtacaag accgtgccga cgacgatcat gatcatagta gtgcctgtat ccatgacgat
     9241 ggccgtctgc cattcatgca ggataacatt ccatcccata gccagcgcta accaaaaaaa
     9301 agcggtgagc atggcgttga ataaggccgt gagtaccaca cgaagccggc gatccgatac
     9361 gagcggcgag gaacggctga cataatacgc cgaaagattg atgaatccgt aaatcaaagt
     9421 gagcggtatc gcaaatgcaa aagcttgcgt cccgtctccg cgtccggtaa aatagatcag
     9481 catggcgatc aacgcgcctg caaaaagcca caccagtatg taccatgcaa ggctgcgaat
     9541 gttgccgagt acaggatgca ttagtttcga acctcaacgc cgcccatgat ggcatatcct
     9601 tggatgtgca agcgtttggt ctgatctgcg ggatgcagtg ttttgtcatc aaagccgccg
     9661 agaatcggtg tgccgttgag aaataccgcc cagtccgtcg gtactttgat atcaatgccg
     9721 ccgaataatg caaacacgtg gatcgtcgca tcatcggcta cggaagcatg acgcaaatct
     9781 atctcgcatc cacccatgac ggccgttact tcgccgcctt taaaatcctg actggcaatg
     9841 atacgtgagt tgccaccgaa aattgccgtc gcttgtacga cgtgttcatt atcggctttt
     9901 ggtatgttcg tttcgtcctg tccacgatgg cgataaaaac ctttgagtat aaaagcaccg
     9961 ccggcgagga tcataaaaac cggccaccat gtgtgccaac tgaaatagat gatcccgagt
    10021 ctgtggagaa tcatgatcgt accgacgcac atcaatacgc tgccgacaaa ataactcggt
    10081 acggtacgcg attggcgaat tttgaggacg ccgaaaataa taaagataac cggccataaa
    10141 cgcgaaagcc tgaagtcttc tataattcgg aggttgtcca ggagaaataa aacgccgaga
    10201 acgatggcga taactccgag gataacctgc ccggggattt tagagtccgt tttcataatg
    10261 tgtcgtagtt tgtggtgtaa aataatattt gtttaataaa cttctggtga ttttactgac
    10321 gccccaggcg atcaataaaa acggccaact gttgcgatag gtcaatcccc atgcataaaa
    10381 ctgtaccgcg agcaaccagc cgccggttaa tatccaaaac acgccatcgg cgatgcgacg
    10441 cgccttgttg taactgagta aatcgctgat accgcctacg atcaaaaata caggccagaa
    10501 tttccacaac ggtccgatgt catacacgtg caatcggtct aataacacga cgaaaccgat
    10561 tgcgatcagg gcaacgcccc agatcaggtg cctttttatg tcatgagagt gatgcgtgaa
    10621 catggctttt tccttgtttt tgtttacgcc ataaacatac ataggaaaac aaattttgtt
    10681 gcacgcattt cggtgaatgg cggcggggag tcggtgaacg gcgacgcgat caagtatatg
    10741 ttgcaaaaaa gcgcatataa atggatgtct tttttttaga aaggcaaata tgcgccctca
    10801 tgcagcggta ttatttcaca caatgctttt aagcgttcaa gttattctgg agaaaccgca
    10861 gaacctgatc cattgcaata cgttcctgcc tcatgctgtc gcggtgacgg acggtgacgg
    10921 tttgattttg cagcgtctcg ctatcgatcg taaagcaata cggcgtaccg atttcatctt
    10981 gccggcggta gcgttttccg acggtggcgg attcatcgta aaatatctta taatgtttct
    11041 taagttcatt atataaagtc atagaaactt ccggcatacc atcttttttg accaaaggca
    11101 aaatagcggc tttgaccggt gcgagcgtgg ggtgaaactt catcacgacg cgggtttccg
    11161 ttttaccgtc gccggtaggt gcttcttctt cggcatacgc attgaccagc gaagccatga
    11221 caccgcgcga agcgcccgcc
//
LOCUS       DAOQNQ010000079        11134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2082_length_11134_cov_5.305984, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000079 DAOQNQ010000000
VERSION     DAOQNQ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11134
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2082_length_11134_cov_5.305984"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /gene="rsmI"
                     /locus_tag="PK754_06755"
     CDS             <1..585
                     /gene="rsmI"
                     /locus_tag="PK754_06755"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNB09239.1"
                     /translation="DHYGIMARTVSLFEHNELRRIPEILQLLMSGKSVAVVSDAGTPT
                     ISDPAFKLVRAVREAGLPVSAVPGPSAALAAISVSGLPTDRFVFEGFLPHKKGRQTRW
                     KTFAEEDRTIVLYESPFRVMKTLSEAREYLGERPMVVAREITKKFEETVSGTPSELIA
                     HFEKHAPRGEFVILIAGKKYAEKALMSEEENEEN"
     gene            617..1282
                     /locus_tag="PK754_06760"
     CDS             617..1282
                     /locus_tag="PK754_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNB09240.1"
                     /translation="MEQLIYLPVLILAVVVHECAHGLVAKWCGDDTAYREGRITLNPI
                     SHVDLWGTIIVPLVLALSSAGLMFGWAKPVPVNPNNFRHFRRDDILVSMAGPASNFII
                     AFISALFIVLLSLVWKAASPESASSMQFGEFALELFKHSIQINLYLALLNLIPIPPLD
                     GSHVIANLLPPHLGEQYRSLGFIGIFILIVMISIGVINAAIDPFIEYLIGLLNAFIVM
                     LAS"
     gene            1288..2319
                     /locus_tag="PK754_06765"
     CDS             1288..2319
                     /locus_tag="PK754_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HNB09241.1"
                     /translation="MQLQYESRTLHLRHPFIISGSQYSEKVVFTATLMHEGIVGRGES
                     SPSRYYGEPPESVQTMLSDTARWIGDISSPADIQTLHETLLQKYPHQPSARSCVIMAA
                     YDWLARSCQMPLYRLWGLNPHKACRTSFTIGMDEPDVLTEKVKEAEAYPVLKIKLGNG
                     DEDYTIMQTIRRCTDKTIRVDANEGWQRDEAIEKITWLATQNVEYIEQPLPKESLEDM
                     VWLKARSPLPLIADENVMTSRDIPGLHHAFHGINLKLDKCGGMWEALRMIQTARALDM
                     KIMIGCMVSTSLAMTAAAHLTPLTDEADLDGHLLIRDDPFRGVTINHHGQLILPESDG
                     IGVTEQGIH"
     gene            2584..3687
                     /locus_tag="PK754_06770"
     CDS             2584..3687
                     /locus_tag="PK754_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNB09242.1"
                     /translation="MIQIGEILKQVPFFRSLGRDSIDFITEKLKFKQYKNGTPICKIG
                     DPGDKMWIVLSGKVAVSAANGEVLAQIGQGNYFGEMALLTGEPRSATVSAADDTETFI
                     LDKDDFDVILEKYPSISIAMSKIMSQRLRETNAVVSEKAKELKSQAPAGPSGSLRDKH
                     ITEVLAFCEANSLTGALEVSNAGQTAVIEFEKGVLQTIKFGTMGEDEAMDTLMGWEDG
                     TFVVKPRLIALDGLGSGSSTETPKHESKKTGSILVINHSMVVRKVLEKNLSSLGHTVK
                     TADGITDGIGALRTVKPNVIISDFKYDEISGYDFCMQIRETANYLDIPFIFITDSSTD
                     SAVVNQLKSVSNSAVVDAQDVSHIANTIEKMLP"
     gene            complement(3866..4597)
                     /locus_tag="PK754_06775"
     CDS             complement(3866..4597)
                     /locus_tag="PK754_06775"
                     /inference="COORDINATES: protein motif:HMM:NF014346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB09243.1"
                     /translation="MRSLLMIAFLSVASYAQSQNWQWPDSSKNLQVLPSNTRGTELRK
                     TMSEFINGLGVRCVYCHVGEDNKSLSTFDFVSDAKETKNTARKMMTMVKVINETYIAH
                     ANENKSKPLDVTCVTCHRGAPLPERIEDILASTYATSGIDSVKNQYRSLRSTYYGSFT
                     YDFREGVLLRTASRIKKATDPSDAIALVELNNEHFPNSVRTLITLATLHQEAKHIDVA
                     KSYAKKVLEIEPGNEAAKKLIESLK"
     gene            complement(4666..5961)
                     /locus_tag="PK754_06780"
     CDS             complement(4666..5961)
                     /locus_tag="PK754_06780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04013 family B12-binding domain/radical SAM
                     domain-containing protein"
                     /protein_id="HNB09244.1"
                     /translation="MQALTLIIYYKGVNTYALNTLIGAVQQGIEDPRLHIALAFRRDE
                     LYSKALSAYNQGHRVVVSWSFYSTHSTGVQEDLHWFREQPHTSDITHLAGGVHASSEP
                     HATLRMGFDWVAMGEGEMIIVELLRRLLNGDDVYTTHGLAYIHGETWKRNPPPPLIQL
                     DDFPPFADRWVRVNPIEITRGCIYACSFCQTPFMMKARFRHRSVASVMRYVQIMKENG
                     AWDIRFITPTSLSYGSDDASVKLEAIEALLKGVRQVLGDRGRIYFGTFPSEVRPEHVT
                     PDILRVLKKYVHNDNLLMGAQSGSQNMLDRMHRGHDVSCIEQAVRYSVEAGFLPNVDF
                     IFGFPDETEEDITASLQLAERLTDMGARIHGHTFMPLPGTPLRSSSPGHISERIIEKL
                     HKFISAGKMYGQWIAQRNMASELSKKNSRKEPARATCRS"
     gene            6094..9429
                     /locus_tag="PK754_06785"
     CDS             6094..9429
                     /locus_tag="PK754_06785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB09245.1"
                     /translation="MLRFLVSLILIFVLKPTAHVLALDPQRTLSQYVQSSWTTETGLP
                     QNTIHAIAQSHEGYIFFGTYEGLVRFDGVHFTVFDRRNTPQLLNNGILSLLYTRDSTL
                     WIGTNGGGLTRLHRGLFTTIDINSGLPHRIVTAIAEGEDGTLWIGTAGGGLAAYYKGE
                     IKVISTDQGLTQLTVNALCVTKSGMLWIGTNGGLFSIPGSRFSDISGYTVTRHSDPNG
                     SSLTGIQAITESPSGYMLIGTNGSGVYTYRHGAWSSITLRGALTVGAVYAIRSDPDGS
                     VWIGTSGSGLKRIAFIQINSEGLPQSYTVDEAFTNQSVWSLFVDRDGHIWIGTNGGLH
                     RLMNGPFTTYTTQDGLPDDYIRAVTMDRDNDLWFGSRGGLTRMQWVQGKPVWRTFTPK
                     DGLSNISILSLFSEPDQLWVGTYGGGLNRRSGEQTQVYTESHGLGSNVIRAIARDVYG
                     NLWVGTSGGISMALAPDLKHAQPHNMLFRKFSSRDGLSNDHVYTILPDADGSVWVGTI
                     SGLNRISEGRIQTWHSGAFSSNILAMFLEGDSVLWVGTDGGLVRVNKSWLLKNTKIPT
                     QNKTSELTTLTVNEGLHSDKIFTILFKSPCDLWMSSNRGVFMICKDQVTAFIRGEIPA
                     VHSSYYGIEDGLKTIQCNGASQSAGIITSDGTMWFATAGGLSSIKTESLTENAQVLLP
                     IAEYIIADQKQYWIPADASSIDLPPGTQHVELHYTALDFAEASKIRFQYRLDGLDENW
                     HLAGSRRVAYYTNVPPGSYVFEVKAASAMGNWSSKPTAIRIHVQAFFYQTLWFYALCA
                     VMAAVCAWGLYHWRVRVLTTRQRELQKIITQRTTELKESQAQLVHSEKMAALGRMVGG
                     IAHEVNNPLTFIYGNLHFLHAHQATFRQLLKLIEQGGTAEQNQNMLKILSDAGYASLD
                     EALNETITLIDQFKHGADRIRNIVINLKHFASVESSEDESVDVHEGLEATIKLLQAQN
                     QLPIEYYKDFTDIPKVHGLSGEINQVFMNVLMNAVQAIEGQWRKGVTRSGHIRIRTES
                     CQLYAKEAIRIRIRDDGEGISEEHRSKIFDPFFTTRTVGKGMGLGLTVCYGIIERHKG
                     RIYLNPDQREETEIIIELPVKTE"
     gene            complement(9584..9958)
                     /locus_tag="PK754_06790"
     CDS             complement(9584..9958)
                     /locus_tag="PK754_06790"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNB09246.1"
                     /translation="MKWIVLVAMIFSVNSFAQENKPKYEMREYFFVMLKKGPNRSQDS
                     VTAMKLQEGHMGNITRMADEGKLAIAGPFGDNGDWRGILILTVKTMEEAKTEVEKDPA
                     VQAGRLIYEIHPWWSARGSKLP"
     gene            complement(9992..>11134)
                     /locus_tag="PK754_06795"
     CDS             complement(9992..>11134)
                     /locus_tag="PK754_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HNB09247.1"
                     /translation="KKLDKADHFELTQVAVFFGEPDKTVPDPYFDGKGPERAGCNYCG
                     GCMLGCRFNAKNTLDKNYLHLAQNLGVKIQAETEAIAVTPLNDNNGNDGYRITIRSST
                     RWFSKPKTVTTKGVIFAGGVMGTVPLLLKLKKEHLPRLSDRIGCQARTNSESLLGVTT
                     FDKNTNFSTGIAISSILHTDEHSHIEPVRYPAGSGAWRILGSPMTAGKNWVTRLSKAL
                     WDILRHPIRNARIYFVSDWARRTQILLFMQTINSTLRFSLGSWGMKSTMEQGVAPTVF
                     MPEAQSLAKHFAEETNGKPVALLTETLLGIPTTAHILGGCVMGRDASEGVIDKDHRVF
                     GYENMYVCDGSAISANPGVNPSLTITALTERAMSKIPNASDILNSL"
BASE COUNT         2856 a         2506 c         2742 g         3030 t
ORIGIN      
        1 gatcattacg gtatcatggc acgaacggtc agtctttttg aacataatga actccggcga
       61 ataccggaga tattacaact gctgatgtcc ggtaaatccg tggccgtggt ttccgatgcg
      121 ggtacaccga cgatttcaga tccggcattc aagttggtac gtgccgtgcg ggaagcgggt
      181 ttaccggttt ccgccgtgcc cggacccagc gccgctttgg cggctatatc ggtgagcggt
      241 ttacccacgg accgatttgt attcgaaggt tttttaccgc ataaaaaagg ccgccaaaca
      301 cgatggaaaa cgttcgcgga agaagaccgc acgatcgttt tgtacgaatc gccatttcgt
      361 gtgatgaaaa cactgtcgga ggcgcgcgaa tatctcggcg aacgccctat ggtcgtagcg
      421 cgcgagataa ccaaaaaatt cgaagaaacg gtttcaggta cgccgtcgga attgatcgct
      481 cattttgaaa agcatgcacc acgcggcgaa tttgttatcc tgattgccgg gaagaaatac
      541 gccgaaaaag cgttgatgtc ggaagaagaa aacgaagaaa attaagtttg taaataaata
      601 cagaaaggtt acgtcattgg aacaactcat ttatctgccg gttttgattt tagccgtcgt
      661 cgtacacgaa tgtgcgcacg gacttgtagc taaatggtgc ggagacgata cggcctatcg
      721 cgaagggcgt attacactca atccgatcag ccatgttgat ttatggggaa cgatcatcgt
      781 gcctttggta ctggcattga gcagtgcagg actgatgttt ggttgggcca aacccgtgcc
      841 cgtaaatcct aacaattttc gtcactttcg cagagacgat attttagttt cgatggccgg
      901 acccgcatct aattttatca tcgcattcat atcggcgctt ttcatcgttt tgttgtcctt
      961 agtatggaag gccgcatcac cggaatccgc gtcaagtatg caattcggtg aatttgcttt
     1021 ggaacttttc aaacatagca tacagatcaa tttgtatctt gctttgctta atctaattcc
     1081 gatacctcca ctggatgggt cacatgtcat tgcaaacctg ttgccgcctc atctcggcga
     1141 acaatatcgg agcttaggtt ttattggaat atttattttg attgttatga ttagtattgg
     1201 tgtgatcaat gcagcgattg acccattcat cgagtatttg attgggttgc tcaacgcgtt
     1261 tatcgttatg ttggcgagtt agattgcatg caacttcagt atgaatcacg tacgctccat
     1321 ttgcggcatc cgtttattat ttcgggttcg cagtattcgg aaaaagttgt ttttacggcg
     1381 acgctgatgc atgaaggtat cgtaggacgc ggcgaatctt cgccatcgcg ttactatggt
     1441 gaaccgccgg aatccgtaca gacgatgtta tccgacaccg cacgatggat cggtgacata
     1501 tcctcgcccg cggatattca aactttgcac gaaacgcttc ttcaaaaata tccgcatcag
     1561 ccgtcagccc gctcgtgtgt gatcatggcg gcctacgatt ggcttgcacg atcctgtcag
     1621 atgccgctgt accggctttg gggattaaat cctcataagg cgtgccgcac atcatttact
     1681 atcggtatgg acgaacccga tgtgttgacc gaaaaagtca aagaagcgga ggcgtaccct
     1741 gttttgaaaa taaaactggg gaacggcgat gaagattaca ccatcatgca gaccattcga
     1801 cgctgtaccg ataaaacgat tcgtgtagac gccaacgaag gctggcaacg tgacgaagcc
     1861 atcgaaaaaa tcacctggct tgccactcaa aacgtagagt acatcgaaca gcctctgccg
     1921 aaagaatcgc tggaggatat ggtatggctc aaagcgcgtt caccattacc tctgattgcc
     1981 gatgaaaacg tcatgacgtc gcgggatata ccgggattgc atcacgcatt tcacgggatc
     2041 aatctcaaat tggacaaatg cggcggaatg tgggaggctt tacgtatgat acaaacggcc
     2101 cgcgctttgg atatgaaaat aatgatcggc tgtatggtca gcacttcgct ggccatgacg
     2161 gccgcggcgc atctgacacc gctgacggac gaagccgatc tggacggcca tcttttgatt
     2221 cgcgacgatc cttttcgtgg tgtaacgatc aatcatcatg gtcaacttat tttaccggaa
     2281 agcgatggga tcggcgtcac agaacaaggt attcattagc cgtgttaccg cgattaccca
     2341 agcatcgtca tttttcatac acaccgcgcc ggtataaacc ggaagcggaa ccgacgtctc
     2401 gtaaatctat ccgctttcaa cgtaatgata cccgcaaacc ctcgattttt cgatggctat
     2461 gggtagtact tattatactt tcgctttggt tgattttaaa aaatcttgct atacaatagc
     2521 aaaaatgtta ctttcacgcg tttatcagaa ttttttttgt gattcttctt cggagtttat
     2581 ggaatgatac aaataggcga gattctcaaa caagtacctt tctttcgctc gctaggtcgc
     2641 gatagcattg attttataac cgaaaagctc aagttcaaac aatacaagaa cggaactccc
     2701 atttgtaaaa tcggcgatcc cggtgataaa atgtggatcg ttttgagcgg taaagtcgct
     2761 gtcagcgcag cgaacggtga agttttagca caaatcgggc aaggcaatta tttcggtgaa
     2821 atggcgttgc tcaccggtga accgcgttcg gccactgttt cggcggcgga tgatacggaa
     2881 acatttattt tggacaagga tgactttgat gtcattttag aaaagtatcc ttcgatctcc
     2941 attgccatga gcaaaatcat gagccagcgt ctccgcgaga cgaacgccgt ggtttccgaa
     3001 aaagccaaag agcttaaatc gcaagcgccc gcgggaccat ccggcagtct gcgcgataaa
     3061 catatcaccg aagtcctggc tttttgcgaa gctaattcgc ttaccggtgc actcgaagta
     3121 tccaatgcgg gtcaaacggc cgtgatcgaa tttgaaaaag gcgttttgca aacgattaag
     3181 tttggcacga tgggtgaaga cgaggcaatg gatacgctga tgggatggga agacggtaca
     3241 ttcgtcgtca agccgcgtct gattgcgttg gacggactgg gctccggcag ttcaacggaa
     3301 acacccaaac atgaaagcaa aaaaaccggt tcaatactcg tgatcaatca cagtatggtg
     3361 gttcgcaaag ttcttgaaaa aaatctaagc agtcttggtc acacggtcaa aacggcggac
     3421 gggattaccg acgggatcgg cgcgttgcga accgtcaaac ctaatgtgat tatttcagat
     3481 ttcaaatatg atgaaatttc cggatacgat ttttgtatgc aaatccgtga gacggctaat
     3541 tatttggata taccgtttat tttcatcacc gattcgagta cggattcggc ggttgtgaac
     3601 caattaaaaa gcgtgtctaa ttcggccgtg gtggatgcgc aagacgtttc gcatatcgcc
     3661 aataccattg aaaaaatgct gccttagttc ataaggcgtt aaaattaaga agctgtatcc
     3721 ggcagttgtt tgagatcatt tcgtgccatt gcatacattc tgttataact acaataatgg
     3781 aaactcgtat caacagacac gactgagtcg aatgccagat accgcttctt tttttatgca
     3841 aatttttgac acgtcgtatt ttttattatt tcaggctctc tattaatttt ttagcggctt
     3901 cgtttccggg ttcgatttcc aaaacttttt tagcatagga ttttgccaca tcaatgtgtt
     3961 tggcttcttg atgaagtgtg gcgagcgtta taagcgttcg tacagaattg gggaaatgct
     4021 cattattgag ttccaccaaa gcgatagcat cggaagggtc ggttgctttt ttgatgcgtg
     4081 aagccgtacg caacagcaca ccttctctga aatcatacgt aaaacttcca taatatgtgc
     4141 tgcgcagtga gcggtattgg tttttgaccg aatcaatacc tgatgtcgca tacgtcgaag
     4201 cgaggatatc ttcaatgcgt tccggcagcg gtgcgccacg atggcatgtg acgcaagtga
     4261 catcgagtgg tttggatttg ttttcattcg catgggcgat ataagtttcg ttgatgactt
     4321 tgaccatcgt catcattttc cgtgccgtat ttttagtttc tttggcatcg gagacaaaat
     4381 caaacgtcga gagcgatttg ttatcttcac cgacatggca atacacgcaa cgaacaccaa
     4441 gcccattgat aaattcagac atggtttttc ttaattcggt gccacgtgta tttgacggaa
     4501 gaacctgcag gttttttgat gagtctggcc attgccaatt ttgtgattgg gcatacgatg
     4561 cgacagataa aaaagcaatc attaagagac tacgcataga aggctcctga agttaagtgg
     4621 tagggtataa gggtataata ggtgtaatca gttcctaata cgtaattagg acctgcaggt
     4681 tgcacgggcg ggttcttttc tggagttttt tttagacaat tctgacgcca tgttacgttg
     4741 tgcgatccac tggccgtaca tttttccggc tgaaatgaat ttatgtaatt tttcgattat
     4801 gcgttccgaa atatggcctg gcgaagatga tcgaagcggt gtgccgggca gcggcataaa
     4861 cgtatgcccg tgaattcgag cacccatatc ggttaatcgt tccgccaatt gcagcgaagc
     4921 cgtgatatcc tcttctgttt catcagggaa tccgaaaata aaatccacat tgggtaaaaa
     4981 accggcttcg acggagtaac gcacggcctg ttcgatgcag gagacgtcgt gcccgcgatg
     5041 catgcggtcg agcatatttt gtgaaccgga ttgagcgccc atgagcagat tgtcattgtg
     5101 cacatatttt tttaataccc ggaggatatc cggcgtcacg tgttccggcc ttacctccga
     5161 aggaaacgtt ccaaaataaa tacgtccgcg atcacccaga acctgtcgca cgcctttcag
     5221 taacgcctca atggcctcga gtttgaccga tgcatcatcg gaaccgtagg acaaggatgt
     5281 cggtgtaata aaccgaatgt cccatgcgcc gttttctttc ataatctgaa cataacgcat
     5341 taccgaagcg acggaacgat ggcgaaagcg cgctttcatc ataaaggggg tctggcaaaa
     5401 actacatgca taaatgcatc cgcgggttat ctcaatagga ttgacacgta cccatcggtc
     5461 tgcaaacggc gggaaatcat cgagctgtat caacggcggc ggcggattac gtttccatgt
     5521 ttctccgtgg atataagcca atccgtgtgt ggtgtagaca tcatctccat ttaataaacg
     5581 ccgcagcaat tcgacgatga tcatttcacc ctcgcccatc gcgacccaat caaatcccat
     5641 acgaagcgtg gcgtgcggct cggagctggc atgcacgcct cccgcgaggt gtgttatgtc
     5701 ggatgtatgc ggctgttccc gaaaccaatg gaggtcttct tgtacgccgg tactatgcgt
     5761 ggaataaaaa gaccaactta cgacgacgcg gtggccttga ttatacgccg atagagcttt
     5821 ggaatacagt tcgtcgcgtc gaaaagctaa tgcgatatgc aggcgcggat cttcaatgcc
     5881 ctgctgtacg gccccgataa gagtattcag cgcgtacgta ttgacacctt tgtaatatat
     5941 aataagtgtg agcgcttgca taaaatgata ttcataaatt tttagacgac aaacaatgtt
     6001 aaaaactata ccgagctatt gttttatata gtcaaaataa tgatattgtt ataggttttc
     6061 acatcgcggg ttctaatttt tttatataaa attatgttgc gttttctcgt ctctttgatc
     6121 ttaatctttg tgctaaagcc aaccgcgcac gtgttggcgc ttgatcctca gcggacgctg
     6181 tcgcaatatg ttcagtcttc gtggaccacg gagacaggcc ttcctcaaaa cacaattcac
     6241 gctattgcgc aatcgcacga aggttatatt ttcttcggga cttacgaagg cttagtacga
     6301 ttcgacggtg tacattttac ggtatttgat cggcgtaata cgcctcagct tttgaataac
     6361 ggtattttat ctttgttgta tacgcgcgat agcacattgt ggatcggtac caatggcggc
     6421 ggtttgaccc gtttgcatcg cggactgttt acgacgatag atatcaattc cgggctcccg
     6481 catcgcatcg tgacggctat agccgaagga gaagacggaa ccttatggat cggcacggcg
     6541 ggtggcggat tggcggcgta ctacaaggga gagatcaaag tgatttctac cgatcaaggc
     6601 cttactcagc ttacggttaa tgctctgtgt gtgactaaaa gcggcatgtt gtggattgga
     6661 accaacggag gattattcag tataccgggc agtcgttttt ccgatatatc gggttatacc
     6721 gttacgcggc actccgatcc gaacggttcc tcattgacgg gtattcaagc tatcacggaa
     6781 agcccttcgg gctatatgct gatcggtacc aatggttccg gcgtctatac gtatcggcat
     6841 ggtgcatggt cttctatcac tttacgtggt gcgctgaccg tcggagcggt gtatgccatt
     6901 cgttccgatc ccgacggctc ggtttggatc ggtaccagcg gaagcggatt aaagcgtatt
     6961 gcattcattc aaatcaacag cgaaggtttg ccgcagtcct atacggtaga cgaagcgttt
     7021 accaatcaat cggtctggag tttattcgta gatcgcgacg ggcatatctg gatcggtacc
     7081 aatggcggat tgcatcgcct gatgaacggc ccatttacga cctatactac gcaagacggg
     7141 ttgcccgatg attacattcg cgccgttact atggatcggg ataatgactt gtggtttggt
     7201 tcgcgcggcg gattgacacg catgcaatgg gtgcaaggta agccggtatg gcgcacgttt
     7261 acgccgaaag acggactttc gaatatttcc attttgtcgc ttttttccga gcccgatcaa
     7321 ttgtgggtcg gaacgtacgg cggcggactc aatcgccgtt ccggtgaaca aacgcaagta
     7381 tatacagaga gtcacggttt gggaagtaat gtcatccgag cgattgcaag agatgtgtat
     7441 ggtaatcttt gggtaggcac atccggagga atatctatgg cgttagcgcc ggacttgaaa
     7501 catgcacagc cgcacaatat gctcttccga aaattctctt cacgcgacgg tttatccaat
     7561 gatcacgtat atacgatttt gccggatgcg gacggcagcg tttgggtcgg tacgatttcc
     7621 ggtctcaacc gtatcagcga aggtcgtatt caaacatggc atagcggcgc tttttcatcg
     7681 aatatattgg cgatgttcct cgagggggat tccgttttat gggtcggtac ggacggcggg
     7741 ttggtgcggg tcaataaatc atggctgctc aaaaacacaa aaattccgac acaaaataaa
     7801 acgagtgaat tgacaacttt aacagtgaat gagggattac actccgataa aatttttacg
     7861 atactattta aatcgccgtg cgatctgtgg atgagctcca accgcggcgt ttttatgata
     7921 tgcaaagatc aggtaaccgc ctttattcgc ggtgaaatcc ccgcggttca cagttcatac
     7981 tacggtattg aggacggcct caaaacgatt caatgcaacg gcgcttcgca atcagcgggt
     8041 attatcactt cggatggtac gatgtggttt gcgacggccg gcggcctgag cagcatcaaa
     8101 acggaatcgt tgaccgaaaa tgcgcaggtt ttacttccga tagccgaata tatcatcgcc
     8161 gatcaaaaac agtactggat tcccgctgac gcgtcgtcta ttgatttacc cccgggcacg
     8221 caacatgttg agttgcatta tacggccttg gattttgctg aagccagtaa aatccgtttt
     8281 caatatcgtt tggatggatt ggatgaaaat tggcatctgg ccggatcgcg tcgcgtggcg
     8341 tattacacca atgtgcctcc gggttcctat gtgtttgagg tcaaagccgc gtcggctatg
     8401 ggtaattggt cttccaagcc gacggcgatt cgtatccacg tccaagcctt tttttatcag
     8461 acactgtggt tttatgcatt gtgcgccgtg atggccgccg tatgcgcatg gggattgtat
     8521 cactggcgtg tgcgcgtttt gaccacaaga caacgcgaat tgcaaaaaat aataactcaa
     8581 cgtacgacag aactaaaaga atcgcaggcg caattagttc actctgaaaa aatggcggcc
     8641 ctcggtcgta tggtgggcgg tattgcgcat gaggttaata atcctctgac cttcatttac
     8701 ggcaaccttc attttttaca tgcccatcaa gcgacgtttc gccaattact caaactgatc
     8761 gaacagggtg gaacagcgga acaaaatcaa aatatgctca agattttatc agatgccggt
     8821 tatgcctcgc tggatgaagc cttgaatgaa acgattacac taattgatca gtttaagcac
     8881 ggtgcagatc gtattcgtaa tatcgtaatc aaccttaaac attttgccag cgtcgaatca
     8941 tcggaagatg agtccgtaga tgtgcatgaa gggcttgagg cgacgatcaa acttctgcaa
     9001 gcgcagaacc aactgcccat cgaatactat aaagatttta cggacattcc caaagtgcac
     9061 ggattgagcg gagaaatcaa tcaggtgttt atgaatgtat tgatgaatgc cgtacaagcg
     9121 atcgaggggc agtggcgcaa aggtgttacg cgatccggtc atattcgcat tcgcacggag
     9181 tcatgtcagc tttatgccaa agaagcgatt cgcatacgca ttcgcgatga tggagagggt
     9241 atatcggaag agcatcggtc taaaattttc gatccttttt tcacgacacg tacggtcggc
     9301 aaaggaatgg gtttgggact caccgtttgt tatgggatta tcgaacgcca taagggaagg
     9361 atatacctca atccggatca acgcgaagaa accgagatca tcatcgagct ccccgtcaaa
     9421 accgaataga attgaatttc gatttctctc ttaacggctt tttttcaccg atccagtgcg
     9481 aaggcggccg taatgctgat gaaaatggta agccactccg ccgtcaattt tttccatgtt
     9541 gagcttgtat cagccatttt gagaattctc cattatatta cgattacggt agtttagagc
     9601 cgcgagcgct ccaccaagga tggatttcat atatcaatcg tccggcctgt acggcggggt
     9661 ctttctccac ttcggtttta gcttcttcca tcgttttgac ggtcagaatg agaatgccgc
     9721 gccagtcacc gttatcgccg aacggtcctg caatggcgag tttaccttcg tcggccatgc
     9781 gcgtaatatt acccatatgc ccttcctgca atttcatagc cgtcaccgaa tcttgactac
     9841 gattaggacc ttttttgagc atcacaaaaa aatactcacg catttcgtac ttaggtttgt
     9901 tttcctgagc aaaactattt accgaaaaaa tcattgcgac caacacgatc catttcatag
     9961 gaaatcctca tttagattat gttaaatgta tttatagtga atttaatatg tccgatgcat
    10021 tcgggatttt actcatggcg cgttcggtca acgctgtgat cgtaagcgac gggttgacac
    10081 cgggattggc ggagatcgca gaaccgtcac acacgtacat gttttcgtat ccaaaaacac
    10141 gatgatcttt gtcaataacg ccttcggagg catctcggcc catgacacat ccgcctaata
    10201 tgtgtgctgt ggtcggaatg ccgagcaatg tttccgtaag cagtgcgacc ggtttaccgt
    10261 tggtttcttc ggcaaaatgt tttgccagtg attgcgcttc gggcatgaat acggtaggcg
    10321 caacgccttg ttccattgtg gatttcatac cccatgatcc gagagaaaag cgcagtgtgc
    10381 tattgatcgt ctgcataaaa agaaggatct gcgtgcgccg tgcccaatct gaaacgaaat
    10441 atatccgcgc attgcgtatg ggatggcgaa gtatatccca caaggctttg gataaacgtg
    10501 taacccagtt ttttccggcg gtcatgggtg aaccaagtat cctccatgca ccggaacccg
    10561 ccgggtaccg taccggttcg atatggctgt gctcatccgt atgtaaaatg gaactgatag
    10621 ctatgcctgt ggaaaaattg gtatttttat caaatgtcgt tactccaagt aagctttcgg
    10681 aattggtgcg agcctgacat ccaatgcgat ccgacaagcg cggtaagtgt tcttttttca
    10741 gtttaagcaa taaaggtacc gtgcccataa cgccaccggc gaaaatgaca ccttttgtgg
    10801 ttacggtttt tggtttagaa aaccaccggg tcgaagagcg tatcgtgatg cgataaccgt
    10861 cgttgccgtt gttatcgttc agaggagtga cggctatcgc ttccgtttcc gcttggatct
    10921 tcacgccgag attctgagcg agatgtaaat aatttttatc taatgtattt ttagcgttga
    10981 atcggcatcc gagcatacat ccgccgcaat agttgcagcc tgcgcgttcc gggccttttc
    11041 cgtcaaaata agggtctggc acggttttgt caggttcgcc gaaaaaaacg gccacttgcg
    11101 ttaattcgaa atggtcggct ttatccagtt tctt
//
LOCUS       DAOQNQ010000080        11130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2083_length_11130_cov_5.246862, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000080 DAOQNQ010000000
VERSION     DAOQNQ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11130
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2083_length_11130_cov_5.246862"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1904
                     /locus_tag="PK754_06800"
     CDS             <1..1904
                     /locus_tag="PK754_06800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNB09248.1"
                     /translation="TNKLIWGDNKLILSSLANGPMREEIEKEGGLKLIYIDPPFAVGT
                     DFGFDIEIGGETAEKKQSIIEEIAYRDTWGKGISSYLSMMYERLKLMHNLLARDGSMF
                     VHCDYRVDSHLRLILDDLFQADRFINSIQWQRTYTTGSSKSISNKFPTNTDTILYYRK
                     SDRYTFNKEYREYSEGTIRRYDKVDEDGKRYKWENLKSYSQKTLQDLLDKGEARFNEG
                     SKYPVYKAYLDEDKGTVVDSLWSDLSHSGTFGDDKLGYPTQKPEALLERIIKASSNEG
                     DLIADFFCGSGTTAAVAEKLGRKWITTDLGRFSVHTARKRLIGVQRELQASGKDFRAF
                     ELLNLGKYERQFFMDDLTNGKRKAKEDLYVDLILEAYKAKRIDGHAHLHGHKAGRFVN
                     VGPLDVPVTQSRLMDIFEECRTKLYTQVDVLGFEFEMGLTPQFIQELKEKGVSVTLKY
                     IPKDVFDKRAVEKGQVKFFDVAYLNTKEKIKGKSITIELTDFVTHYTQDDIEELQQSM
                     RAGSTVVIEDGQIFKIEKEKNGIVTRTVLTKNWFDWIDYWAIDFNYEDKKEIIKIKNE
                     KGEIEEKWSGNYLFENEWQSFRTKKNPTLEFTSIAHEYKSAGKYKVMVKVVDILGVDT
                     SKIIEVSIK"
     gene            2345..2857
                     /locus_tag="PK754_06805"
     CDS             2345..2857
                     /locus_tag="PK754_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNB09249.1"
                     /translation="MAAIHSDASQFFDKPRNKSMILKKAFLLLITASLATFLQAQQLL
                     TKDQQEAQQTVVKMFEALSKRDSVNLKAHCTADITLYEYGQVWNIDTLTHKAITKNTD
                     PDFNRTNTFEFINTETDTHTAWVTYRLKSTITKDSKQTTIQWLESVFLEKQKNQWKVK
                     HLHSTLMSRN"
     gene            3112..4701
                     /locus_tag="PK754_06810"
     CDS             3112..4701
                     /locus_tag="PK754_06810"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015025137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HNB09250.1"
                     /translation="MKTYPSIFNDVIGPVMRGPSSSHCAASLRIGRIGRDLMDGDIRE
                     VYIEFDPDGSLATTHKGQGSDMGLFGGFLGWEAYEERLPDYQQAVDEAGIDIKIAIHP
                     IGATHPNTYKITLSNASETRELTAISTGGGMIEVIEIDGAQVRMAGDFYQTLVYVKSP
                     GEIISFIEKSFPCDEIGLRKGKSTFIEIKSNQFLPDPLCRELQSIDDVLFIKKLNPVL
                     PVMSRKDLTVPFITCEEMLAYNKGKNLELWELAVDYESMRGNITRQEVFEKMRAIVEI
                     MQNAIALGLKGTTYGDRILGAQSVEFQQQMKDKKLIEGDVLNRVILYTSAMMEVKSSM
                     GVVVAAPTAGSCGALPGAVIGLSDALHFSQDEMVKAMLAAGIIGVFIAAHATFAAEVG
                     GCMAECGSGSGMAAAGIVGIKKGTLDQALAASSMALQSSLGMICDMIADRVEAPCLNR
                     NVMAATNALSCANMALSNYNHLIPLDEVIETMKKVGDAIPNTLRCTGLGGLAITKTAK
                     KIEATLEKNQAESNKKFFKVC"
     gene            4798..5007
                     /locus_tag="PK754_06815"
     CDS             4798..5007
                     /locus_tag="PK754_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNB09251.1"
                     /translation="MDNAKTKKLKNGDPCIVIGGTHKGKAGIVQDLNTSKTGHITITV
                     LQSNGVRFKTLGRNVEVVKSKKNAP"
     gene            5192..5452
                     /locus_tag="PK754_06820"
     CDS             5192..5452
                     /locus_tag="PK754_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006527789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNB09252.1"
                     /translation="MSKLEKILLTVLRGTSDTNIDFEDLRKLLHTLGFSERIKGSHHI
                     FYKDGVVEIINLQSKNSKAKPYQVKQVRNLIIKYKLGGDENG"
     gene            5445..5654
                     /locus_tag="PK754_06825"
     CDS             5445..5654
                     /locus_tag="PK754_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNB09253.1"
                     /translation="MDKYEIILFWSEEDNSYVAEVPELPGCAADGATYHEALTNLEVS
                     IKEWIETAKVLGRDIPKPKGRLMYA"
     gene            5904..6341
                     /locus_tag="PK754_06830"
     CDS             5904..6341
                     /locus_tag="PK754_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB09254.1"
                     /translation="MASINPHINFNGNAEEAFNFYKSVFGGAFAKIIRFKDLASADFP
                     VAEHEANKIMHIALPIGKSILMANDVPEILGRTNENENRSKIVVSAESKEEADKIFHG
                     LSAGGQIEMPISDSPWGSYFGMFRDKYGIEWMVDFDARYKGQI"
     gene            6440..7321
                     /locus_tag="PK754_06835"
     CDS             6440..7321
                     /locus_tag="PK754_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNB09255.1"
                     /translation="MSAAKISIRFFNDREVRAVWDEQNAKWWFSVLDIVAVLTDQDDY
                     TKARNYWKYLKAKLKKERSQVVSATTQLKLLAPDGKKRMSDMLDYNGIIALGKTFPGT
                     RANRFIEWFTYSNESIDGKSKTKAYALFESSFINSIEVGTTKGLQQIHAYLFGGLYEF
                     AGQIRKKNISKSGFRFAVSGFLGETLKQIEKMPETTFDEIVNKYIEMNIAHPFMEGNG
                     RSARIWLDLILKKRLKKCVDWSQISKRDYMNAMMQSPTKNDALKKLLKKALTTKINDR
                     EMFMKGIDYSYYYEEND"
     gene            7659..8711
                     /locus_tag="PK754_06840"
     CDS             7659..8711
                     /locus_tag="PK754_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNB09256.1"
                     /translation="MPLRYMLAFFCVSALLFGQDKKPSIAVLDFKASEGLTRGETATL
                     TNRFRGILVQTEAFNVVEQEKMNEILKAQDFSMSDACNTAECAVQVGQLLGVERMIAG
                     DIGKIGDVFTIDLRLIDVSTGKIVKTVTRDYEGKISGLLGVMKDVASTFAAIKPVSPA
                     VGSITIQSTPSKATVSMDGKELNFVTPFVAENLTLGVHEIALRLGTMKAVKTIEIKEG
                     KNPVLNLRLEPITAPLSVASDPAGATVFVNGDRYGDTPLQIKLPIGSHTLTVKQKEYE
                     VFTQSIVLKENDPPLVIQASLTKKIKTLSESSSNKKWWYILGGAAVLGGGTAWLLSGG
                     GKSSGKSGIPPAEFPN"
     gene            8755..>11130
                     /locus_tag="PK754_06845"
     CDS             8755..>11130
                     /locus_tag="PK754_06845"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNB09257.1"
                     /translation="MRYTITIAAVLARVFFCVTLLPSFIFSQGGAWSSKTSMTTARWH
                     SGIGVINNTLYVAGGYNGSHQTVVEAYNFTSNTWTTKNTRPVQTSAAYGVIGNTLYTA
                     GGTNVSVRISDVWAYDPGLDSWTTRAPIPNLRSNPGSAVVNNMLYAIGGVDNSSVLSS
                     MDAYDPSGNAWASKAAMSVPRQAPAVGVIQGIIYVAGGFTTGNAVTDVLEAYNPVTNT
                     WSTKAVLPTARGYAVAAVLDDKLYVMGGVTVGGGSAIQTVEVYDPVTDTWSGGTPMKT
                     GRAGMEAFTYDNKIYAIGGWSGVSTASSILEAFNGRPVIFSFTPAFSPVDSIVTITGN
                     NFSAVPQENVVYFGSVKANVNTASITQLTVTVPAGQNFKPISVTVGGYTAFAKKSFFV
                     TKKVKSTIDESSFDTRIELTSGNNPKNVASGDIDGDGKPDLIISNFDDNTISVYRNIS
                     STGTLSAGSFSGPVDFNTSLSPHGMKIADMDGDGKLDVILSHSSSSFVTVLRNTATSG
                     VINASSFASAISFASGGGSQELTVSDIDGDGRPDIIAANTNGTISVFRNRNTPGSIGT
                     QGFAPYIDLAAGANSESVSAADIDGDNKPDIIFTNRNDNTLSIYRNQSTPDTLTASSF
                     SGLVNFTAATGPGKIAVADFDGDLKTDIATVNPGSATFSVFRNTATPGLINTSSLADR
                     VDFMPGAYQISLAAGDMNSDLKPDIISVAATLSKNNTSAGSITSGSFSTAFTFNVGTQ
                     PNDVELADLDGDGLLDVSMLDYVDKKLYIYRNAMAIVPRMADSLALVDLYDS"
BASE COUNT         3443 a         2418 c         2495 g         2774 t
ORIGIN      
        1 ggacgaataa actcatttgg ggcgataaca aacttatact ttcctcattg gcaaacggcc
       61 caatgcggga agaaatcgaa aaagaaggcg gactgaaact catttacata gacccacctt
      121 ttgccgttgg tacagatttt ggttttgata tagagattgg cggcgaaaca gccgaaaaaa
      181 aacaaagcat tattgaagaa attgcctacc gtgatacgtg gggaaaaggc atttcgtctt
      241 atttatctat gatgtatgaa aggctgaaat tgatgcacaa tttgttggcg agggatggaa
      301 gtatgtttgt gcattgcgat tacagagtag atagtcatct tcgattaatc cttgatgatt
      361 tatttcaagc ggataggttt atcaattcaa ttcagtggca aagaacatat acgacaggaa
      421 gtagtaaatc tatttccaat aaatttccaa cgaatacaga tacaatcctt tattatagga
      481 aaagcgatag atacaccttc aacaaagaat atcgagaata ttccgaaggg acaatacggc
      541 gatatgacaa ggtagatgaa gacggcaaga gatacaaatg ggagaattta aaatcatatt
      601 cccaaaaaac attgcaagac ttattagata aaggagaggc aagatttaat gaaggttcaa
      661 aatatccagt atataaagca tacttagatg aagataaggg aaccgtagtt gatagcttgt
      721 ggtccgattt atcacattca gggacgtttg gagatgataa acttggatac ccaacacaga
      781 aaccagaagc attattggaa cgcatcatca aagcaagcag caatgaaggt gatttaattg
      841 ccgacttctt ttgtggaagt ggaacaacag cagcggttgc agaaaaattg gggcgtaagt
      901 ggataaccac tgatttggga aggttttcag ttcacaccgc aagaaagcgt ttaattggtg
      961 ttcaaagaga attgcaagca agtggaaaag atttcagggc ttttgaattg ctaaaccttg
     1021 gtaaatacga acgccagttt tttatggacg accttaccaa cggtaaacgc aaagccaaag
     1081 aagatttata tgttgattta attttagaag cctacaaagc caaacgaata gacggtcatg
     1141 cacatttaca tggtcacaaa gccggtcggt ttgtaaatgt tggcccttta gatgtgccgg
     1201 taacacaaag ccgtttgatg gatatttttg aagaatgccg caccaaactt tacacccaag
     1261 ttgatgtgtt aggttttgaa tttgaaatgg ggttaacgcc gcaattcatt caggaattga
     1321 aagagaaagg cgtttctgta actttgaaat acattccaaa agatgtgttt gataaacgag
     1381 ccgttgaaaa aggtcaagtg aaattctttg atgtggctta cttaaatacc aaagaaaaaa
     1441 taaaaggtaa atctataaca attgaactca cggattttgt tacacattac acccaggatg
     1501 acattgaaga actgcaacaa tccatgcgag caggaagcac agttgtcatt gaagacgggc
     1561 aaattttcaa aattgaaaaa gaaaaaaacg gaattgttac ccgaactgtt ctaacaaaaa
     1621 actggttcga ctggattgat tattgggcaa ttgactttaa ctacgaagac aaaaaggaaa
     1681 tcatcaaaat caaaaacgaa aagggcgaaa ttgaggaaaa atggagtggc aactatttgt
     1741 ttgaaaacga atggcaaagt ttccgcacca agaaaaaccc tacactcgaa tttacaagca
     1801 tagcccacga atacaaatca gcaggtaaat ataaagtaat ggtcaaagtg gtggatattt
     1861 tgggagtgga cacgagtaaa ataattgaag taagtataaa ataactatag caaacgatgt
     1921 tcacaaagtc ttcgtcagct atcatcacac aatgagcggg caataaaaaa acaatcaaaa
     1981 agtagtcggc ctgacgtaca tgcaccgtca cagcagcgcg tcatcggccc ctttccaata
     2041 atgtcaagtt ttatgaagct tttggtatca agcccggcga cgcgatgtac agacccgaaa
     2101 acgaacgcgt aaatattttg tagatcggcg gtatttactg ttaatacgta aaaaaaaatc
     2161 tttggtgcca tgccggggat tttttgtgaa aggaaataag taaagggatg acatgactta
     2221 gaatggcatt tccaaaggtg actgctgggt aacagttttg tgcttagaat ctcgcccgaa
     2281 cgcaaccccc acaaaccgtt tacaatccac tccaccatcc accactccgc caagccggaa
     2341 aacgttggcg gctatacatt cggacgcctc acaatttttt gataaaccaa gaaataaatc
     2401 aatgatattg aaaaaagcat ttcttctact cattacagct tctcttgcga catttttgca
     2461 ggcacaacag ctattgacaa aagaccagca agaagctcag caaaccgttg taaaaatgtt
     2521 tgaagcttta tcaaaacgag attcggtaaa tcttaaagcc cattgcactg ccgacattac
     2581 tttgtatgaa tacggacagg tttggaatat tgataccttg acccataaag caatcaccaa
     2641 aaataccgat cccgatttta atcgtaccaa tacttttgaa ttcatcaaca ccgaaacaga
     2701 tacccataca gcatgggtaa cataccgact caagtcaaca atcaccaaag acagcaaaca
     2761 aactaccata caatggcttg aatcagtatt tttagaaaaa caaaaaaatc aatggaaggt
     2821 aaagcatctc cattctactc taatgagtcg aaattaaaga ttcaaccccg caatccccca
     2881 ctccggcaag ccgcaaaccg tttctaaggc ctatgatttc gtcagcctcc tactagggcc
     2941 gctctattct tttgaaaatt cgattgacaa atggggtttt agttcccaca ttatacgact
     3001 atttgtaacc cctcacggct tattttccat gaaaaaaatc agaagctaac tcaaggcgat
     3061 gaggttgtcc catcatgcta ctggatgtaa actaacaatg aaaaaacaac gatgaaaacc
     3121 tatcccagta ttttcaacga tgtcatcggt ccggtcatgc gagggccatc cagttcgcat
     3181 tgtgccgcgt cgctgcggat aggccgcata ggccgtgacc tgatggacgg tgatatccgg
     3241 gaagtgtata tcgaatttga tcccgacggc tcgctcgcaa ccacacacaa aggccaaggt
     3301 tcggatatgg ggttgtttgg cggatttttg ggatgggaag cctatgagga acgcctaccc
     3361 gactatcagc aggccgtaga tgaagccggc atagacataa agatcgcgat tcatcccatc
     3421 ggcgctaccc accccaatac gtacaaaata acattaagca atgcgtctga aacaagggaa
     3481 ctcaccgcca tctctaccgg cggcggaatg atcgaagtca tagaaatcga tggcgcgcaa
     3541 gtccgcatgg ccggagactt ttatcaaacg ctggtttacg tgaagtcgcc cggcgagatt
     3601 atttcgttca tcgaaaaatc gtttccatgc gatgagatcg gattgcgaaa aggaaaatca
     3661 actttcatcg aaatcaaatc caatcagttt ctccccgatc cactctgtcg cgaattgcaa
     3721 tccatagacg atgtgctttt tataaaaaaa ttgaacccgg tcctgccggt gatgtcacga
     3781 aaagacttaa ccgttccttt tatcacatgc gaagaaatgc tggcatataa caaaggaaaa
     3841 aacctggaat tgtgggaact ggccgttgac tatgaaagca tgcgcggtaa tatcacgagg
     3901 caggaagttt ttgaaaaaat gcgtgccatc gtagagatca tgcaaaacgc cattgctctt
     3961 ggcctaaaag gaacgaccta cggcgatcgt atattaggcg cgcaatctgt agaatttcaa
     4021 cagcagatga aggacaagaa gttgatcgaa ggcgatgtac taaaccgcgt gatactctat
     4081 acgtcggcca tgatggaagt gaaaagttcg atgggcgtgg tcgtggcggc ccctacggcg
     4141 ggatcgtgcg gcgcattacc cggggctgtt atcggtttga gcgatgcgct ccatttttcg
     4201 caagacgaga tggtaaaagc gatgctggcg gccggtatca taggcgtatt tattgcggcg
     4261 catgctacgt ttgcggccga ggtgggcggc tgtatggccg aatgcggatc cggatcaggc
     4321 atggcggcgg ccggtatcgt gggaataaaa aaaggcacgc tcgatcaggc gctggcagct
     4381 tcttcgatgg cattgcagtc gtctttaggc atgatttgcg acatgatcgc cgaccgcgta
     4441 gaggcgccct gcttgaatag aaacgtcatg gccgcaacca acgcattgtc ctgcgccaat
     4501 atggccttgt ccaactataa tcaccttatt ccattggacg aagtgattga aaccatgaaa
     4561 aaggtgggcg acgccatacc caatacattg cggtgtaccg gactgggcgg cctggctatc
     4621 accaaaactg ccaaaaaaat agaagcgacg ctcgaaaaga accaagcgga gagcaacaag
     4681 aagtttttta aggtgtgtta atccaccctt ttttttcatc cgcatatctt ttcggtacga
     4741 cgaaattgta ctctacgatt tcccattatt aataatgaaa cattgaaagc gcatactatg
     4801 gacaatgcca aaaccaaaaa actaaaaaac ggagacccct gtattgtcat cggcggcaca
     4861 cataagggaa aagccggcat tgtgcaagac ctgaatacca gtaaaacggg tcacattact
     4921 atcactgttt tgcaaagtaa tggcgttaga tttaagactc ttggcagaaa tgtggaagtc
     4981 gtaaagagca aaaaaaacgc cccgtaacac gggttttgtt tcaggcaagt ggcacgcctg
     5041 tacatgcaat aaaactaccc tgccctcgcc aaacccgaaa acgttagatg gtaaggcgtg
     5101 tatattgcat ccgtaatttt tttcatttca catggaacag tttttttata cttttactta
     5161 ttgtttttaa caaacgggat accgtgtata aatgagtaag ttagagaaaa ttttattaac
     5221 tgttttacgc ggtacatcgg atacgaatat agactttgaa gacttacgga aactcttaca
     5281 tacattagga ttttctgagc gaataaaagg gagtcatcac atattttaca aagacggtgt
     5341 ggttgaaatt attaatcttc agtctaaaaa ttcaaaagca aagccatacc aagttaagca
     5401 ggttcgaaat ttgattatta aatacaagct tggaggagac gaaaatggat aaatatgaaa
     5461 ttattttgtt ttggagtgaa gaggataatt cctatgtcgc agaagttcct gaacttcccg
     5521 gatgtgccgc agacggggca acatatcacg aagcactaac gaaccttgag gtttctatta
     5581 aagaatggat tgaaacggct aaagtgttgg gtagggatat tccaaaacca aaaggtcgtt
     5641 taatgtacgc ctgaaaacgt cctcattgcc ctgcatcaca tagtgatacg gtaatcccct
     5701 aacacgatac tcgtagaata tgaaaaatag agttttttat agataatgaa ggattgctaa
     5761 taatataatc cggtcttgca ccgtgccgat gcctcggtcg tcgtacttcg acacattaca
     5821 aactaactta cctaaaaaac acgacccctg aaaatttttg gcttcaacgt taaaaagtta
     5881 aatcaactga aagagttacc ggtatggcga gtatcaatcc ccacatcaat ttcaacggca
     5941 atgcggaaga agctttcaat ttttacaaat ccgtatttgg cggtgcgttc gcaaaaataa
     6001 ttcgcttcaa agatttggcc agcgctgact ttccggtcgc cgaacatgag gccaataaaa
     6061 taatgcacat cgctttaccg atcggtaaaa gtatattaat ggccaatgat gtacccgaaa
     6121 tattgggacg gactaacgaa aatgagaacc gaagcaaaat tgtagtcagc gcagaaagca
     6181 aagaagaagc cgacaagata ttccatgggc tttcggcggg tggacaaatt gaaatgccta
     6241 tttctgacag cccttggggt tcctattttg gtatgtttag agacaaatat ggaatcgagt
     6301 ggatggtaga ttttgatgca agatacaagg gacagatata gccgataacc ttgacagcat
     6361 ctacaaaaaa taatcactca atgattgaat cattgaatta caaggcaact tgaataaagt
     6421 taatatacaa acgcgtatta tgagcgctgc caaaatatcc atacgttttt ttaatgaccg
     6481 tgaagtgcga gccgtatggg acgaacaaaa tgctaagtgg tggttttcag tgttagatat
     6541 tgtagcggta cttaccgatc aagatgatta taccaaagct cgtaactact ggaaatacct
     6601 gaaagccaag ctcaaaaaag agcgtagtca agtggttagt gccactaccc agttgaaatt
     6661 actggcgccg gatggcaaaa aacgcatgtc tgacatgctt gactataacg gtatcatagc
     6721 cttaggaaaa acttttcccg gaacaagagc caaccggttt atcgaatggt ttacctacag
     6781 taatgaaagt atagacggaa aaagtaaaac caaggcctat gccctttttg aaagctcttt
     6841 catcaacagc atagaagtag gcaccaccaa gggattgcaa cagatacatg cttatctgtt
     6901 tggcggcttg tatgaattcg caggacaaat ccggaaaaaa aacatttcaa aaagcggttt
     6961 ccgatttgcc gtttcaggtt ttttaggtga aactttaaaa caaatagaaa aaatgcccga
     7021 gactactttt gacgaaatag taaataaata tatagaaatg aacattgcac accctttcat
     7081 ggaaggcaat ggccgtagcg cccgaatctg gctggatttg attttaaaaa agcgtctcaa
     7141 aaaatgtgta gattggagcc aaataagtaa acgcgactat atgaacgcaa tgatgcaaag
     7201 tccgacaaaa aacgatgcgc ttaaaaagct tttaaaaaaa gcactgacaa ccaaaatcaa
     7261 tgatcgggaa atgtttatga aaggtattga ctattcgtat tactacgaag aaaacgatta
     7321 aaagaaaatt gccttcaacc atcacaaact ttgctcttaa aatatacccc gcgcttgttg
     7381 taccctctcc acgccactga cttttcgccg cattcctcgt cttgtttacc gggcgcgatg
     7441 tatagctccc aatgattaaa aattgttggt tttctaacga caatcattta cttttgtcgt
     7501 ataatctcat ttatcgcata ctcttctttc tgattgtaat cacctgttcg gagcctgcgc
     7561 cctccggtta attttaggcg ttgattgatt tgacgacggc attttaaaaa aacggctttt
     7621 gatagcatag ttttgtaaaa atgaatcgga ggaaaatcat gcccctgcgt tacatgcttg
     7681 cgtttttttg tgtgagcgcc cttctcttcg gccaagacaa aaaaccctcc atcgccgtgc
     7741 tggattttaa agcatccgaa ggattgaccc gcggagagac cgctacgctg actaaccgtt
     7801 ttcggggaat actcgtacag accgaagcct ttaatgtcgt cgagcaggaa aaaatgaatg
     7861 aaatcctgaa agctcaggat tttagcatgt ccgatgcctg caataccgcc gaatgcgccg
     7921 tacaagtcgg gcaactgctc ggtgtcgaac gcatgatcgc cggcgacatc ggcaaaatcg
     7981 gcgacgtctt cacgattgat ttacggttga ttgatgtttc gacgggaaaa atcgtaaaaa
     8041 ccgtcacacg cgattacgaa ggaaaaattt ccggtttgct cggcgtgatg aaagatgtgg
     8101 cgagtacatt tgccgcgatc aaaccggttt cccccgccgt cggctccatc accatccagt
     8161 ccacaccgtc caaagcgacg gtctccatgg acggaaaaga attaaacttc gtgacgcctt
     8221 ttgtagccga aaacctgacg ttgggcgttc atgagatcgc actgcgttta gggacgatga
     8281 aagccgtcaa aaccatagag atcaaagaag gaaaaaatcc ggtattaaat ctacgattgg
     8341 agccgatcac ggcgccgctc tccgtcgctt ccgatcccgc cggagcgacc gtttttgtca
     8401 atggcgatcg ctacggagac acgcctttgc aaataaagtt acctatcgga tcgcatacgc
     8461 tcaccgtcaa acaaaaagag tacgaagtgt tcacccaaag tatcgttctc aaagaaaatg
     8521 atccgccttt ggtcattcag gcttcactca caaaaaaaat aaaaactctg tccgaatcct
     8581 cttccaacaa aaaatggtgg tacatactgg gcggggcagc cgtattgggc ggcggtaccg
     8641 cgtggctctt gagcggcggc ggcaaaagtt ccggaaaatc aggcattcct ccggccgagt
     8701 ttccgaatta atagttttgc aaaacatcat ttaaaaatac ttaaggcaat ctgtatgcgc
     8761 tatacgataa ccattgcagc cgttttagcc cgcgtgtttt tttgtgtgac actcctcccg
     8821 tcatttattt tttctcaagg cggagcttgg tcgtctaaaa cgtccatgac cacggcacgt
     8881 tggcattccg gcatcggcgt gatcaataat acgctctatg tggccggcgg ctacaacggt
     8941 tcgcatcaga cggtcgtcga agcgtataat tttacgagca atacgtggac cacaaaaaat
     9001 acgcgcccgg tacaaacatc cgccgcgtac ggtgtgatcg gtaatacgct ctacacggcc
     9061 ggcggcacga acgtgagtgt acgcatatcg gacgtgtggg cttacgatcc ggggctcgat
     9121 tcgtggacga cgcgtgcacc gatccccaac ctgcgctcta atcccggaag cgcggtggtg
     9181 aataacatgc tatacgccat cggcggcgta gataacagct ctgtactttc ctctatggat
     9241 gcgtatgatc cttccggaaa tgcatgggca tccaaagccg ctatgagcgt tccgcggcaa
     9301 gccccggcgg tcggcgtgat ccaaggcatc atctatgtgg ccggcggttt cacgacggga
     9361 aatgccgtaa ccgatgtgct ggaagcatac aatccggtaa ccaatacatg gtccacaaaa
     9421 gccgtactgc ctacggcgcg cggatatgcc gtcgcggcgg tactcgatga caaactttac
     9481 gtcatgggcg gtgtcaccgt aggtggcgga agcgctattc agaccgtcga agtttacgat
     9541 ccggtaaccg acacctggtc gggcggcaca cccatgaaaa ccggacgcgc aggaatggaa
     9601 gcttttacct atgataacaa aatttacgcc atcggcggat ggtccggcgt cagtaccgct
     9661 tcatccatat tggaagcgtt caacggccga ccggtgattt tttcatttac tccggcattc
     9721 agccccgtgg attcgatcgt tacgatcacg ggaaataatt tcagcgccgt accgcaggaa
     9781 aatgtcgtgt attttggctc ggtaaaagcc aacgtcaata cggcttcgat cacccaattg
     9841 acggttaccg tgccggccgg acaaaacttc aaaccgatca gcgtcacggt gggcggttat
     9901 acggcgtttg caaaaaaatc tttttttgtc acgaaaaaag taaaaagtac gatagatgaa
     9961 tcttcatttg atacgcggat tgaattgacg tcaggaaata atcctaaaaa tgtagcctcc
    10021 ggtgatattg acggcgacgg taaaccggat ctgatcattt ccaattttga tgacaatacg
    10081 atttccgtat atcgaaatat atcctcgacg ggaactctca gcgcaggatc attttctgga
    10141 ccggtagatt ttaatacatc attaagcccg catggcatga aaatcgccga tatggacggc
    10201 gacggaaaat tagacgtcat cctatcgcac agcagcagca gttttgttac cgtccttcgc
    10261 aataccgcga caagcggcgt tatcaatgct tcatcgtttg cttcagcgat aagttttgca
    10321 tccggtggcg gatcgcaaga actgaccgta agcgatatag atggcgacgg acgccctgat
    10381 attatcgccg ccaataccaa cggtaccatc tcggttttcc gcaatcgaaa cacacccggt
    10441 tctatcggaa cacaaggatt tgccccgtat attgatttag ccgccggagc caattcagag
    10501 tccgtatccg ctgcggatat agacggtgat aataagccgg atattatctt taccaatcgt
    10561 aacgacaata ctttgtccat atacagaaat caatccacac cggatacttt gacggcatct
    10621 tcattctccg gtttggtaaa cttcacggcg gcaacgggac cgggaaaaat agcggtcgcc
    10681 gattttgacg gtgatctcaa aacggatatt gcaactgtta acccgggatc ggcaaccttt
    10741 tcagtattcc gaaataccgc aacgccgggt cttatcaata cttcgtcatt ggccgaccgc
    10801 gttgatttta tgccgggtgc gtatcagatt tcactggctg ccggtgacat gaatagcgac
    10861 ttaaaacccg atatcatcag tgtcgcagcg acactaagta aaaacaacac cagcgcggga
    10921 tccattacct ccggctcatt tagcaccgca ttcacattca acgtaggaac acaacccaac
    10981 gatgtcgaat tggccgatct cgatggcgac ggattgctgg acgtatccat gctagattac
    11041 gttgataaaa aattgtacat ctatcgtaat gctatggcca tcgtaccgcg aatggccgat
    11101 tcattggcat tggtggattt atacgatagc
//
LOCUS       DAOQNQ010000081        11075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2099_length_11075_cov_5.381397, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000081 DAOQNQ010000000
VERSION     DAOQNQ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11075
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2099_length_11075_cov_5.381397"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PK754_06850"
     CDS             complement(<1..446)
                     /locus_tag="PK754_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNB09258.1"
                     /translation="MEVNIKKLEKEKIFLEGTIKKFYSTNNPDYLLMECKNDLVPMDT
                     KKVMKARGKGQINTELTSYVFQFLESYHIPTHYVGKYDDKSLVVRNLEMIPIYVVVRN
                     IAAGSFAKNFKMSEGDPLPAPIIEFYFKNDKMGNPMVNEYHLYAFGS"
     gene            complement(487..1785)
                     /gene="purB"
                     /locus_tag="PK754_06855"
     CDS             complement(487..1785)
                     /gene="purB"
                     /locus_tag="PK754_06855"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNB09259.1"
                     /translation="MITRYTRPELGKIWSDENKFQKWLDVEIAVCEAQADLGYIPGDA
                     LAAIKSRARFDTARVLEIEAEVKHDVIAFLTNVAEYVGEPSRFIHLGMTSNDVLDTSL
                     SLTIREAGNILLQDIQQLLDVLHRRALEFKMTPCMGRTHGVHAEPVTFGLKLALWYDE
                     MTRNAERLKRAVEAISVAKISGAVGTFEHLDPRVEEKTASILGLKPINATQVVPRDLH
                     ADFLVTLAVIGASLEKFATEIRSLQRTEVLEAEEYFSKGQKGSSAMPHKRNPITCERV
                     AGLARVLRGNAQAALENVALWHERDITHSSVERVIIPDSTILLDYMLTQFIRVVDKLI
                     VYPENMMRNIERSKGLIFSQPVLLALAKKGLKREEAYKIVQTTAMAVWETPDGNFKKA
                     ITTNAEVTSRLSKEEIDECFDLKHSMKHIGYLFERAGIKS"
     gene            complement(1861..3363)
                     /locus_tag="PK754_06860"
     CDS             complement(1861..3363)
                     /locus_tag="PK754_06860"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB09260.1"
                     /translation="MKLVFKSVAAMILLSLILGQQIFAQEKKKISHSASIQGIKQRLK
                     NASNEPGDADKYYQQALEILLVNNAQVDKNDKEKEETHYYAAAVYHRMNDMIKAWEYC
                     NKTLTFGKKYFEKGEKVSGGIELFSIKDMMNDVKLKTYNQGNQAYRAAAAATSPDSQK
                     VLYHKTISKFKSLLEIDPTVTITANNVPSSYALGVYGTIATVYIQLLNMEKEEANKKT
                     IREDIVSYLTKMHALDRANFSFVTSIISLYTQENNEEKMMAWMDTALAMNAPDSQAVA
                     YQTSLIGDKALMLDRQGKADEAIAVYTKALEKNPENPDLHFNLGRLYYNRKDFDKANT
                     QFRKVKSLRKDDPVVNFQVAEQSYQSYQSKRTETIDKNGGARADMNKITTMLKSDIEI
                     VRTDIADAIASLEASLPTTLEMAETNYRIGKMYNYQAEIEGHLYYTLVNAEKVKKQKP
                     YFEKAIPYLVKASELRPSHANTWIMLGTAYVNLQKKREAEAAFKKANEIK"
     gene            complement(3409..4434)
                     /gene="mtnA"
                     /locus_tag="PK754_06865"
     CDS             complement(3409..4434)
                     /gene="mtnA"
                     /locus_tag="PK754_06865"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNB09261.1"
                     /translation="MIETIRWAHPAVRIIDQTQLPVYETYLTIADLDDLIDAIKKLKI
                     RGAPALGIAGAYGLCLGLYRCTTSDKAILRDEAINTAAILIQSRPTAVNLAWGVHRAL
                     KVFESVYSTASSTDILRSSVLKEAHLILQEELDACIAMGKLGSELIDDGMQVLTHCNT
                     GGLATGGFGTALGVLVTAHQNGKRIHVWVDETRPLLQGARLTSWELKKENIRHTLICD
                     NMAGHLMSKGRVQAVIVGSDRIARNGDAANKIGTYTLAVLCEKHKIPFYVAAPHSTID
                     KQIPDGSAITIEERSADEITMGMGKQTAPQGVHVYNPAFDVTPAELISAIITEKQIFR
                     GPDYCFN"
     gene            complement(4633..5007)
                     /gene="crcB"
                     /locus_tag="PK754_06870"
     CDS             complement(4633..5007)
                     /gene="crcB"
                     /locus_tag="PK754_06870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNB09262.1"
                     /translation="MIQFILIGLGGALGAMARYGLQYVIQQRSGLLFPLGTLIVNLIG
                     CFFIGFIMEIAEIRSAFNPQIRLFITVGLLGGFTTFSAFGYESILMMRTMEFYFSIFY
                     IVGSIVGGLALVILGQTLARLL"
     gene            complement(5041..5904)
                     /locus_tag="PK754_06875"
     CDS             complement(5041..5904)
                     /locus_tag="PK754_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HNB09263.1"
                     /translation="MMRVWKLIVDYGRMIKFSHTIFALPFALTSFVVSGREFPLTLSK
                     FFWIVVAMAGARSAAMGFNRIVDARYDAKNPRTVNREIPRGVITQKQAWIFVVIASTA
                     LVLASYFLNPLCFILSPVALFFVLFYSLTKRFTATAHIFLGIALGIAPLGAWIAMSGQ
                     WDWTAFMLGCAVLTWVSGFDIIYACQDYEFDRSEGLFSIPGKFGIANALWISRFLHAT
                     AIFFLTYVGSHWELGWIYFAGVCIIATLLIYEQSLVHAHDLSKVNIAFMNMNGIISIT
                     YFVFTVLDIYL"
     gene            complement(5901..6476)
                     /locus_tag="PK754_06880"
     CDS             complement(5901..6476)
                     /locus_tag="PK754_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09264.1"
                     /translation="MFKHRVARNRFLYVILLGLIITGFGYLMINYQLKMYQMVLMAAM
                     ILIPNRLARYYWFDFFAGKRELRRKRPEQATIRFRQFLETLRGAPWIKWLMFFSYGLY
                     SFKVEAVALVYLGHAWLYLKKYDDAEKAFRDALLVDDKYVMAKRGLAAVYILRGQNNE
                     AQQWLDLACRFGHPKISFDKFEELTRKEYNA"
     gene            6682..8412
                     /gene="recJ"
                     /locus_tag="PK754_06885"
     CDS             6682..8412
                     /gene="recJ"
                     /locus_tag="PK754_06885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNB09265.1"
                     /translation="MLEQQWVLSNHHDKERVKKLAEEINVPELIAGILFNRGIRTFEE
                     AKNFFRAESAPLNDPFLMHNMDIAVDRVTRAINNRESIMIYGDYDVDGTTSTSMLYLF
                     FKEIHRGFISYYIPDRVKEGYGLSPTGIKYAQEQGIDLLIAVDCGITAIDQVNLANKY
                     GIDVIICDHHHPGDQLPAALAILNPKKESCPYPYKELCGVGVAFKLAQAITQTLKIDE
                     DVLMQHLDLVAIGSAADIVPLTGENRTLVKNGLKLVSDANKVGIRQLVKNANVFGKPI
                     STAQIVFSLAPRINAVGRLGDANRAVKLLVTKSESEAVEFASVLESENRNRRDLNEVM
                     FLEACEIVDQEFDFANDKVIVVYKKGWHQGVIGIVASKLVERYYRPAVVIAEADDKGK
                     GSARSVENFDVFQALKSCEDLMVGYGGHKYAAGLTIDLNNIEEFRRRLNEYAAIHLPP
                     NDLIPKIHIDAEISLDEINNRLMTLLNLFKPHGPANMRPIFISRSLQIVGYPQLMNER
                     HIRMKVRQNGAVFDAVGFNLGHYYNKIGVGDNPLDLVYQIDENTWQGRTTIQLKIKDL
                     KIQTNGNGYH"
     gene            8414..9010
                     /locus_tag="PK754_06890"
     CDS             8414..9010
                     /locus_tag="PK754_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNB09266.1"
                     /translation="MGMQAKPAVFFDRDGTLNVEKNYLYRPEDFEWISGVPEAVHMLN
                     EAGFLVFVITNQAGVARGFYSEQDVNRLHDFMADQLEQKKAHMDAFFYCPHHPEGTIH
                     EYTKLCDCRKPASGMIRQALMRFDIDLSASFVVGDYKSDIELANREVIRSVLVKTGHG
                     QKTIDAMNAGSTACKPDHIAEEVTEAVRWILGQHRERK"
     gene            9010..10170
                     /locus_tag="PK754_06895"
     CDS             9010..10170
                     /locus_tag="PK754_06895"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNB09267.1"
                     /translation="MQYPFSSLSQTLKGSPTVRLAEKAQTLKDAGADIVDLTIGEPDF
                     PTPAHISEAAYRAAQAGNTKYTNSRGILPLRKAIAEKLQRDYSLSYDANTEIIVTPGG
                     KQAVVYVIQAIINPGDEVICIEPCWVGYEGSIAMSGGRYIGVPTYESDSFCIDIDRLR
                     AAVTPKTKLLIINSPSNPTGKILSKREIESICSVCNERNLMLLTDDIYEKILYDNHMF
                     VPAAGLPGMRDRTIILNGFSKAYSMTGWRLAYLAAHKQIISEINKLQQLSATCPSSVS
                     QWAGVEALNGDQSAIQVMTSAYAVRREIVVQGFTKYGLPCFRPEGAFYAWINVKKLGM
                     NSVAACEYILDKCNIAAVPGSAFGISGEGYIRASFATSEENLQKAIKNLENL"
     gene            10212..10286
                     /locus_tag="PK754_06900"
     tRNA            10212..10286
                     /locus_tag="PK754_06900"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10247..10249,aa:Pro,seq:ggg)
     gene            complement(10347..>11075)
                     /locus_tag="PK754_06905"
     CDS             complement(10347..>11075)
                     /locus_tag="PK754_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HNB09268.1"
                     /translation="IIASVVLVSSGLTGEVGMLSALIIGGVVCTALSCAGAFITDLKI
                     GYWLGSSPYKQETWKFAGALVASLTVGWVIMLLNETYGFTGPDALVAPQANAMAAVIQ
                     PLMTGQPAPWMLYVGGAFMAIVLAWLGVPALAFALGMYIPLELNTPLLFGGLVAHWVS
                     TRSTDEKLNNARRERGTLIASGFIAGGALMGVISAILRYAGYNWFNAEWAESHGGELL
                     AGLMFVGILIYMYWDSMRGKPEEE"
BASE COUNT         3155 a         2378 c         2432 g         3110 t
ORIGIN      
        1 gagccgaacg catacaaatg gtattcattt accatcgggt tgcccatttt atcattttta
       61 aaataaaatt caatgatcgg agctggtagg ggatcacctt cgctcatttt aaagtttttg
      121 gcgaaactgc ctgccgcaat attgcgtacc acaacataaa taggaatcat ttcaagattg
      181 cgtaccacga gggatttgtc atcgtatttg ccgacgtagt gcgtggggat atgataactc
      241 tcaagaaact gaaatacata agacgtaagc tctgtgttaa tctgaccttt cccgcgtgct
      301 ttcattactt tttttgtatc cattggcacg agatcatttt tacattccat caacaaataa
      361 tcaggattat ttgttgagta gaatttttta atcgttcctt caagaaatat tttttctttc
      421 tcgagtttct tgatattgac ttccaaaata acctcctgcg gatgccgcac tatacttaaa
      481 ttaattctat gattttattc ctgcgcgttc aaaaagatac ccgatatgtt tcatgctatg
      541 cttcagatca aaacattcat ctatttcttc cttcgatagg cgcgacgtta cttctgcatt
      601 ggtcgtaatg gcttttttga agttaccgtc cggtgtttcc catacggcca tagccgtagt
      661 ttggacgatt ttatacgcct cttcgcgttt tagtcctttt ttggcaagcg ccagcaatac
      721 cggttgactg aagatcaacc ctttggatcg ttcaatattg cgcatcatgt tttccggata
      781 cacgatgagc ttatcaacga cgcgtataaa ttgtgttaac atataatcca gcaaaatagt
      841 gctgtcaggg atgatcacac gctcgacgga agagtgggta atatctcttt catgccacag
      901 cgccacattc tccagtgccg cttgagcatt accgcggaga acgcgagcca atcctgcgac
      961 acgttcgcag gtgattggat tacgtttgtg cggcattgcc gaagatcctt tttggccttt
     1021 agaaaaatat tcttcggctt caagaacttc tgttcgctgt aaactgcgaa tttcagtggc
     1081 aaatttttcc aaagatgcac cgatgacggc cagcgttaca agaaaatcgg catggaggtc
     1141 acgcggcacg acttgtgttg cattgatcgg ttttaagccg agaattgaag ctgttttttc
     1201 ttcaacacgc ggatccaaat gctcaaaggt acctacggct ccggatattt tggcaacact
     1261 gatcgcttcg acagcacgtt tcaaacgctc ggcattgcgc gtcatttcat cataccacag
     1321 tgccagtttt aaaccgaaag taaccggttc agcatgcaca ccgtgcgtcc gtcccataca
     1381 aggtgtcatc ttgaattcca atgcgcggcg atggagtacg tcaagcagct gttggatatc
     1441 ctgaagcaag atgttgccgg cttctcgaat cgtcaacgaa agggaagtat ccaaaacatc
     1501 gttggatgtc ataccaaggt gtataaaacg cgaaggttcg ccgacatatt cggcgacgtt
     1561 tgtaaggaat gcgatcacgt catgcttgac ttcggcttcg atctccaaaa cgcgcgccgt
     1621 gtcaaaacgt gcacgggatt tgatagcggc taatgcatcg ccaggtatat aacccaaatc
     1681 cgcttgcgct tcgcaaacgg ctatctccac gtcaagccat ttttgaaatt tattttcgtc
     1741 cgaccagatt tttcctagct ccggacgtgt atatcgtgtt atcatgcgtt atgttcatcc
     1801 ggcggtatat cggataccaa gtttaccgcc gacgttttgc ctatctgaat tttatctaaa
     1861 ttatttaatt tcgttagctt ttttaaatgc ggcttcggct tcacgctttt tctgcaaatt
     1921 aacataggct gtaccaagca tgatccatgt attcgcatgg gaagggcgca gctcagaggc
     1981 ttttaccaaa tagggaattg ctttttcaaa ataaggtttt tgttttttaa ctttttcggc
     2041 gttgacgagc gtatagtaca gatgcccttc aatttccgcc tggtaattat acatttttcc
     2101 gatacgataa ttcgtttcag ccatttcaag tgtagtcggt aagctggctt ccaatgatgc
     2161 gatagcatca gctatatccg tgcgaacaat ctcaatgtcg gatttgagca tcgtagtgat
     2221 tttgttcata tcggcgcgtg cgccgccgtt tttgtcaatc gtttccgtgc gtttgctctg
     2281 ataggattgg tacgactgtt cagcgacttg gaaatttaca accggatcat ctttacgcag
     2341 gcttttgact ttacggaatt gagtattggc tttatcgaaa tctttacggt tatagtagag
     2401 acgcccgaga ttgaaatgta aatccggatt ttccgggttt ttttctaatg ctttggtata
     2461 cacggcaatg gcttcatccg ctttaccttg acgatcaagc ataagcgctt tatcgccgat
     2521 gagggaggtc tgataagcaa cagcttgact gtccggtgca ttcatggcta aggccgtatc
     2581 catccaagcc atcatttttt cttcattatt ttcttgtgtg taaagactta taatgcttgt
     2641 gacaaatgag aaattagcgc ggtcaagagc atgcatcttg gtaagatatg atacgatgtc
     2701 ttcccgaatg gtttttttat tggcttcttc tttttccata ttaagaagct gaatatatac
     2761 ggtagcaatc gttccataga cgcctaaagc atacgaactc ggtacgttat tggctgtaat
     2821 ggtaacagta ggatcaattt ctagtaatga tttgaatttt gaaatggttt tatgatacaa
     2881 aaccttttgt gagtcagggg atgtggcggc cgctgcagcg cggtaagcct gattgccttg
     2941 gttatatgtt ttcagcttga catcattcat catatctttg atggagaaaa gctcaatgcc
     3001 gcctgaaact ttttctcctt tttcgaagta ttttttgccg aaggttaatg ttttattgca
     3061 atattcccat gctttgatca tatcattcat acgatgataa acggcggcgg catagtagtg
     3121 tgtttcttct ttttctttat cattcttatc aacttgcgcg ttattgacca atagaatttc
     3181 caatgcctgc tgataatatt tgtctgcatc accgggttca ttagaagcat tttttaaacg
     3241 ttgtttgatg ccctgaatag aagcggaatg gctgattttc tttttttcct gcgcgaaaat
     3301 ttgttgacct aaaattagac tcagaagaat catcgcggct acggatttga ataccagttt
     3361 cattagaaat ctccaatcat tagtgtgaat gaaacaatat tatttgtgtt aattgaaaca
     3421 atagtccggg ccacggaata tttgtttctc agttattata gccgaaatca attcggccgg
     3481 tgttacatca aatgcagggt tgtatacatg cacaccttgc ggcgctgttt gttttcccat
     3541 acccatggtt atttcgtcag ctgagcgttc ttcaatggtt atagcgcttc cgtcaggtat
     3601 ttgtttgtca atcgtcgaat gcggagcggc gacataaaat ggtattttat gtttttcgca
     3661 caataccgct aaagtataag ttcctatttt atttgccgca tcaccattac gtgcaattcg
     3721 atctgaacca acgattacag cctgaacgcg acctttggac ataaggtgtc ccgccatatt
     3781 atcgcaaatc aatgtatggc gtatattttc tttttttaat tcccatgatg ttaatcgggc
     3841 gccttgtaaa agtggtcgcg tttcatcaac ccacacatgg attctttttc cgttttggtg
     3901 ggccgtaacc agtacaccta gggctgtacc gaatcctccg gttgcaagtc cgccggtatt
     3961 gcaatgagtc aaaacctgca taccgtcgtc tatgagttca gagcctaatt ttcccatcgc
     4021 aatacacgca tctaactctt cctggaggat aaggtgcgcc tccttaagaa cagaagatcg
     4081 caatatatct gtactcgaag ccgtggaata aacagattcg aaaaccttta gcgcacgatg
     4141 gacgccccac gcaagattaa ctgcggttgg gcgactttgg atcaatatcg cggctgtatt
     4201 gatagcttcg tcgcgaagta ttgctttatc tgacgtggta caacgataaa gtcctaaaca
     4261 caaaccgtat gcaccggcaa ttcctaacgc aggagccccg cgaattttca attttttaat
     4321 tgcatcaatg agatcatcca gatcggcgat tgtgagataa gtctcataaa ccggtaattg
     4381 ggtttgatca atgatgcgta cagcgggatg cgcccaccgt atcgtttcaa tcatattaag
     4441 tttaacaaaa acaaaaacgt aattctgtcg cgaatatatt ggttgtacca taaaaaatca
     4501 agcagtttta cgatttgaca accgttatgt tgaatgccaa tctgacatgg attaacatat
     4561 aatagagcga gtaaacggat agaatattaa caatgaaaaa tagaaaggag tatatggttt
     4621 gttgtaaata aattacaaaa gtctagccaa agtttgacct aagataacaa gtgcaagccc
     4681 gccgacaatg cttccgacta tataaaatat agaaaaataa aattccatcg tacgcatcat
     4741 aagtatgctt tcgtatccaa acgctgaaaa agtagtgaaa ccgccgagca atcccacagt
     4801 gataaataat cggatttgcg gattaaatgc tgaacgtatt tcagctatct ccataatgaa
     4861 cccaataaaa aaacaaccga tgagattgac aatcaaagta cccaaaggaa acaacaaacc
     4921 ggaacgctgt tgtatgacgt attgaagccc ataccttgcc attgcgccca gtgcgccgcc
     4981 taatccgatc agaataaatt gtatcatatt gtaaaattgt aaaattaaca gagttaattt
     5041 ctatagataa atatctaata ccgtaaaaac aaagtaggtg atactgatga tgccattcat
     5101 attcatgaaa gcgatattta ctttggataa atcatgagca tgtactaacg attgttcgta
     5161 tatcagcaaa gtagcaatta tgcacacacc tgcgaaatat atccagccca attcccagtg
     5221 agaaccgacg tatgttagaa aaaaaatggc tgtcgcatgc aagaagcgcg aaatccacag
     5281 agcattggca attccaaatt tgcccggtat cgaaaacaaa ccttcagagc gatcaaattc
     5341 gtaatcttga caagcgtaga tgatatcaaa tccggaaacc caagttaata cggcgcagcc
     5401 tagcatgaat gctgtccaat cccattggcc cgacatagca atccacgctc ccagcggagc
     5461 aattcccagc gcaattccta aaaaaatatg tgctgtagcc gtaaaacgtt tggtaagcga
     5521 ataaaataaa acaaaaaaaa gggcgacggg cgatagtata aaacatagag ggttgagaaa
     5581 gtaggaggcc aaaaccagtg cggttgatgc tataaccaca aatatccatg cttgcttttg
     5641 cgtgataacg ccgcgaggaa tttctcgatt cactgtacgc ggattttttg catcataacg
     5701 cgcgtccacg atacgattga atcccatcgc cgcacttcgt gcgccggcca tcgcaacgac
     5761 gatccaaaaa aatttggaca aggtcagcgg gaactcgcga ccgctgacga caaacgacgt
     5821 taaagcaaac ggcagcgcaa aaatcgtatg agaaaacttg atcatgcgac cgtaatcaac
     5881 aattaacttc caaacgcgca tcatgcgttg tattccttgc gcgtgagttc ttcaaatttg
     5941 tcaaaggaga ttttagggtg accaaaacgg caagccaaat caagccattg ttgcgcttca
     6001 ttattttgtc cgcgtaaaat gtaaacagcg gcgaggcctc gcttagccat gacgtatttg
     6061 tcatccacca agagcgcatc gcggaatgct ttttcggcat catcatattt ttttaaataa
     6121 agccatgcat gcccgagata aactaaagca acagcttcta ctttgaaaga atacaggccg
     6181 tacgaaaaaa acatcaacca ctttatccaa ggagcgccac gcagagtttc caggaattgc
     6241 cgaaaacgta ttgttgcttg ttcaggtcgt ttacgacgca attcgcgttt tcctgcaaaa
     6301 aaatcaaacc aataatagcg tgccaaacga ttggggataa ggatcatggc cgccattaaa
     6361 accatttgat acattttgag ttgatagttg atcattaaat aaccaaaacc cgttatgatc
     6421 agacctaaaa gtatgacata caaaaaacga ttgcgggcaa cgcgatgttt gaacatagtt
     6481 ttttccttga ttgtgcgtac gataccaaaa aaatcaatgc cgtgcaaggt ttgaaaatac
     6541 attttgcatt ttacaaagcg ataaactata ttttatcaag ggtttattta ttatctttcg
     6601 gccctaatga attcaaaagt gggttttttt caactatgat gtttaaaaaa ctaaatataa
     6661 atcaaaccat tacaatccct tatgttggaa caacaatggg tactgagcaa tcatcatgac
     6721 aaggagcgtg taaagaaatt agcggaagaa attaacgtac cggagcttat agccggcatc
     6781 cttttcaatc gtggtattcg tacgtttgaa gaggcaaaaa attttttccg ggcagagagc
     6841 gcaccgctca atgatccttt tcttatgcac aatatggata ttgcggttga tcgtgtaacc
     6901 cgcgcgatca ataaccgtga atccatcatg atttatggcg attatgacgt ggacggtacg
     6961 acgagtacct ctatgttgta tctcttcttc aaagaaattc accgcggatt tatttcctac
     7021 tacattccgg atcgcgtcaa agaagggtat ggactttctc cgacgggcat caagtatgct
     7081 caagagcagg ggattgactt acttattgcg gtagattgtg gtataaccgc tattgatcag
     7141 gtcaatcttg ccaataaata cggcattgat gtgattattt gtgaccatca tcatcccggc
     7201 gatcaattac ccgcggcact agctattctc aaccccaaaa aagaatcgtg cccttatccg
     7261 tacaaagaac tgtgcggcgt gggcgttgca ttcaaattag cacaggcgat cacgcagaca
     7321 ttgaaaatag acgaagatgt gctgatgcaa catcttgatc tggtggcgat cggcagcgct
     7381 gcagacatcg taccgcttac gggcgagaat cgcaccttag tgaaaaacgg gcttaaatta
     7441 gtcagcgatg ccaataaagt cggtattcgc cagctggtca agaatgccaa tgtgttcggc
     7501 aaacctattt caactgcaca gattgttttt tcattagcac cccgtatcaa tgccgtaggc
     7561 cgattgggtg acgctaatag agcagtaaaa ttattggtta ctaaaagtga atccgaagct
     7621 gtcgaatttg ccagtgtttt ggaatccgaa aatcgcaatc gccgtgatct caacgaggtg
     7681 atgtttctcg aagcttgtga gatcgttgat caagagtttg atttcgccaa tgataaagtc
     7741 attgtggtgt ataaaaaagg atggcatcaa ggtgtgatcg gtatcgtggc cagcaaactg
     7801 gttgaacgtt attatcgtcc agccgtggtc atcgccgaag cggacgataa aggtaaaggt
     7861 tcggcacgaa gcgtagaaaa ttttgatgtg tttcaggcgc ttaagtcgtg tgaagatctc
     7921 atggtcggct acggaggtca caaatatgcg gccgggttga ccatagacct caataacata
     7981 gaagagtttc gccgccgact gaatgaatat gctgctattc atcttccgcc caacgatctg
     8041 atcccgaaaa tccatattga tgccgaaatt tcattggatg aaatcaacaa ccgtttgatg
     8101 acattgctta atctttttaa gccgcatggc ccggctaata tgaggccgat atttatatcg
     8161 cgttctttgc agatcgtggg ttatccgcaa ctgatgaatg agcgtcatat ccgaatgaaa
     8221 gttcgccaaa atggcgccgt gtttgacgct gtgggtttca atctagggca ctattacaac
     8281 aaaatcggcg taggtgataa tccgctggat ttggtgtacc agattgatga gaatacctgg
     8341 caaggtcgga cgactataca gcttaagatc aaagatctaa aaattcagac taacggaaac
     8401 ggttatcact agcatgggta tgcaggccaa acccgccgtt ttttttgatc gtgacggtac
     8461 tctgaatgtt gaaaaaaatt acctgtatcg tcctgaagac tttgagtgga tatctggagt
     8521 gccagaggcc gtgcatatgc ttaatgaagc gggttttttg gtttttgtta ttaccaatca
     8581 ggccggggtg gcgcgtggtt tttatagcga acaagatgta aatcgtcttc atgattttat
     8641 ggcggatcag ttagaacaaa aaaaggcaca catggatgcc tttttttatt gcccacatca
     8701 tccggaaggc acgatacacg aatacactaa actatgcgat tgccgcaaac cagcatcggg
     8761 catgattcga caggcgttaa tgagatttga tattgattta agcgcctcgt ttgtggtcgg
     8821 ggattataag tcggatattg agttagcaaa tcgtgaagtc atacgatcgg tactggtaaa
     8881 aactggacat ggacaaaaga caatagatgc gatgaatgcc ggatcaacag cgtgtaaacc
     8941 agatcatata gccgaagaag ttaccgaagc cgttcgatgg atattagggc aacataggga
     9001 gcgtaaataa tgcagtatcc gttttcatcc ttgagccaaa cgctgaaagg ttcacccacc
     9061 gttcggcttg ctgaaaaagc acagacgctg aaagatgcgg gcgctgatat cgtagatctg
     9121 actatcggag aacctgattt tccgacaccg gcgcatatta gcgaggccgc ctatcgtgca
     9181 gcacaagcgg gtaatactaa gtatactaac agccgcggga tacttcctct acgaaaggcg
     9241 atagccgaaa aacttcaacg agattattca ctttcgtatg atgcgaatac agaaatcatc
     9301 gtgactcccg gcggaaaaca agccgtggtg tatgtgatcc aggcaataat caatccgggc
     9361 gatgaagtga tatgtatcga accatgttgg gtgggttacg aaggttctat cgcaatgagc
     9421 ggaggccgtt atatcggcgt acctacgtat gaatcggata gtttttgtat agatattgat
     9481 cgccttcgcg cggcggtgac gcctaaaacc aaattactca tcatcaatag tccgtctaat
     9541 cctaccggta agatcctttc aaaacgggaa atcgaaagta tttgttctgt atgtaatgaa
     9601 cgcaatctga tgcttctgac ggatgatatt tatgaaaaaa tactatacga taatcacatg
     9661 tttgtccctg cagccggttt accgggcatg cgcgatcgta cgatcatact caatggtttt
     9721 tccaaggcat actctatgac gggttggcga ctggcgtatt tggccgccca taaacaaatc
     9781 atatcggaga tcaataaact gcaacagctt tcagcaacat gcccttcgtc ggtgagtcaa
     9841 tgggcgggtg tcgaagcctt gaacggtgat caatccgcta ttcaagtcat gacgtcagct
     9901 tatgctgtac gtcgcgaaat cgtcgtgcag ggatttacta aatatggttt gccatgcttt
     9961 aggccggaag gggcttttta tgcatggatc aatgtgaaaa agttaggtat gaattctgtc
    10021 gccgcatgcg aatatatact tgataaatgc aatatagccg ctgtacccgg aagtgctttt
    10081 ggaatctcgg gcgaaggtta tatccgcgcc tcatttgcta cgtcagaaga aaatttacaa
    10141 aaagctatta aaaaccttga gaatttgtaa tcggtagtat atattgccgc cgttgattga
    10201 ataacaagga tcggaatgtg gcgcagcccg gatagcgcac tcccttgggg tgggaggggt
    10261 cggaggttca aatcctctca ttccgacaaa aaaaagaaaa gccctcatca acgagggctt
    10321 ttctttttgt gatattttta taatctctat tcttcttccg gtttaccacg catcgagtcc
    10381 caatacatat agatcagaat tcccacaaac atcaaaccgg ctaataattc acccccgtgc
    10441 gattcagccc attcggcatt aaaccagtta tatcccgcat agcgtaagat cgcgctgatg
    10501 acgcccatga gtgcgccgcc ggcaatgaag cccgaagcga tgagcgtgcc gcgttcgcga
    10561 cgcgcgttgt taagtttttc gtcggtggaa cgggttgaaa cccagtgcgc aacgaggccg
    10621 ccgaaaagca aaggtgtatt gagttctaag ggaatataca tccctagggc aaaagcgagt
    10681 gccggcacgc ctaaccatgc cagtactatc gccataaacg cacctccgac atacagcatc
    10741 cacggtgccg gttgaccggt catcagcggc tgtatgacgg cagccatggc attggcttgt
    10801 ggtgcaacta atgcatcagg tccggtaaat ccgtaggttt cattgagaag catgattacc
    10861 catcctacag taagcgacgc tactaatgca cccgcaaatt tccatgtctc ttgtttgtac
    10921 ggggaggaac cgagccaata tccgatttta aggtccgtaa taaaagcacc ggcacacgat
    10981 aaagccgtac aaactacgcc gccgatgatc agtgccgaga gcataccgac ttcacctgta
    11041 aggccggatg aaactaatac aacagacgcg atgat
//
LOCUS       DAOQNQ010000082        11060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2103_length_11060_cov_5.285507, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000082 DAOQNQ010000000
VERSION     DAOQNQ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11060
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2103_length_11060_cov_5.285507"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="PK754_06910"
     CDS             <1..906
                     /locus_tag="PK754_06910"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNB09269.1"
                     /translation="ITPPFWQTWWFRLLMLGFAGLIVWGMLKLRSYYAEYRKTHYVSH
                     YKLLEKIGEGGMGVVYKARDRVSGKTVALKLLHESIADTKDGLQRFLKEAEVGKLIHH
                     PNVVKIYGAGSSETRRFITMEMVEGKTLKQTIRERGVFTKDDTKKIIIQLVEGLAQIH
                     VHGVVHRDLKSDNIIIKPDGRVVIMDFGLARIKGMTSVAARDQLVGTLAYMSPEQTIG
                     KEVDHRSDIYSLGVITYEMLYGEMPFIANNEMELILAIHNETPKHIDANNVDALHQCI
                     KICLSKDPIDRYPDTTTLRKFLDTV"
     gene            971..4252
                     /locus_tag="PK754_06915"
     CDS             971..4252
                     /locus_tag="PK754_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNB09270.1"
                     /translation="MSSAQYQLPFVHYTADNQINPLPGVAVYDVYQDRLNFLWISIFS
                     AGLVRYDGHRMEVYTTRDGLRSLNFTTTIEDTDGRLWVMTDGGLSVSEQPLKTYGAHR
                     VRFIDRIGDQPLAKTSISQIAMKVLAQDTAGRIYLGTTGAGILRYSRVKPDFWSCDTI
                     TTQNSHSSRNASVYSMMVDKKGLVWISTDDGVAILKPGGHRIEYLERSIIPPVRVQTF
                     FQTNDETLLAGCEDGSIYQWEKEAHGFRKMDHTLTSPVYDITQDRRGDLWICSDGAGV
                     LRWRKGNSPEHITRRQGLIAESVRFVMEDREGTLWFSQVGGLSKLRANYKAFQSISAD
                     ATLETGVVLPDPNVTGISTEAWTGDKPLLWMGLGKGFAVLTHDGKISHFDAKKGLSSN
                     TVYDVLVDEKKRLWVGTFAGLNSFTFYGTPPVTLGATSSRRVQLNNQPAVMDFHDIGI
                     TGVVSRYAMRENPEAQRTVESLWFNSIRRLSVYVGNSWYIFDEASGLPSSIIYSIAWD
                     SAGWMYVGTGDQGIYRSRFSLTSSELRKQFERSGKGLYQREITTPCFEKYWNQENGAP
                     FDDPQSLLFVGDRLWVGSASRGLVVINPQTRTLIHQMNSDAGFRSPGIINLIFSPVFG
                     TVWCGSSNGIYEIDPISYKLLRSVFRDDGLISSEVNWLTSTAFDSDGKLYIGTPKGMT
                     IYDPKLDRPDLTSPQPSITSATFHQSLGGENELLTEFSALSFTNEKAVQYKVRLVGYE
                     SDWHAVSEPRTRYTNLPAWFTEARYRFEVLASNAHGVWSEMPAVYEFNVMPVWWLRWW
                     AFGIYIALFSALVLGARWFVINWRSVLMPKTRYIAHYKILEKIGRGGMGMVYKARDTL
                     NKRTVAIKILHEEFEQTEDGIKRFIKEAEIGRKLKHPHIVEIYDAGRFDKTRYITMEF
                     IEGVTLKNFLRVHGPLMPADVVRIIRPVAQALRTIHAHDVIHRDLKSDNVMIRNDGVV
                     KIMDFGLARTKGMTTVVQRDQLVGTLAYMSPEQSLGKPVDARSDIYALGVIMFEMVEG
                     CLPFNGQHEMELIYAIHNETPEFHNTNIPEALRQFILRCLEKEPERRLANMDELEEVL
                     NKIAV"
     gene            4319..6061
                     /locus_tag="PK754_06920"
     CDS             4319..6061
                     /locus_tag="PK754_06920"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HNB09271.1"
                     /translation="MIKIYESLQDLQVKIDETFKELSQGFNKVNGAITQKNLPAAESA
                     LNDLFKAFMSRTRAINAQSASVGADIKKIESQCAEISAEKEQFKALYNSGIAMTTQGE
                     LQGLITFAMNQMTYHLRAERGFLILVDDHGEREYFVSKNFDGQNIDDPAAEVSTSVIQ
                     KTLDALQPIKVDDDTLSESIMKQGSFVRLGLRAVMSVPIIFRKKLLGVVYLDRREETK
                     GFSDNDLAFLMAFSRQIAFRVNELKEQQHVHTEYERRDRNRLQSLREKYNFSEIIGKS
                     ERLVKVLELAGKVASSEVTVLIQGESGTGKELIARAIHFNSDRYEHKFIAINCGAIPS
                     ELLESELFGYESGAFTGAVKTKIGKFEAAHRGTLFLDEVAELSMNLQAKILRVLQTKE
                     LERLGSTETRKIDLRIISASNKNLQQMVREGKFREDLYYRLKVVEIELPALRERKEDI
                     HLLVSFFVQKYGEQRIQSVSEDAMDILENYAWHGNIRELENVIQRAIILSSGTQIEVQ
                     DLPPEIVSKTESGYKVDKDLSLEEAETDFRRWFVLRALRKNNNNKSKAAEMLGINRTH
                     FFRMLNQLGISDTE"
     gene            6264..6473
                     /locus_tag="PK754_06925"
     CDS             6264..6473
                     /locus_tag="PK754_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09272.1"
                     /translation="MKKKVLIFNQDIDELSVIRQVLVREGYEAITASNWDTATKLFAS
                     LEIDYVLMNGKSSDIQRVLQNALKM"
     gene            6641..7465
                     /locus_tag="PK754_06930"
     CDS             6641..7465
                     /locus_tag="PK754_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09273.1"
                     /translation="MKHLMTWVYILVLVALTSCGGDDAAKPEEFGTQEEALTALNANK
                     SNMLLALDAAEGAIDAASGFGLDKRSAYNSKKLNRAMTYTYDASTGWWTDEYTYSSGG
                     YNYDYTWNIRFTPRDANGYSTDETSKMEYKYDYLYNGANTYYSYDMDLGADMTLTGVN
                     AYRAATGNLVLNGTNAISYVWDYSYLTYDYTYDYSHKYTYKSLEISPDGDYPEGGSVS
                     FTIRSKFDGEIGEEGGDALNYYVAGKITFDGDNTALLEFGGFSFILNLDTNEVTPA"
     gene            7608..8432
                     /locus_tag="PK754_06935"
     CDS             7608..8432
                     /locus_tag="PK754_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09274.1"
                     /translation="MKNVMRFLAVAMSTFMISCGGDDELKPEKFATQEEAMTAFSNNQ
                     YNLQDAYHSVGDAIDYTGGVSVGAVKTEQKKKMNRAMTWAYDASTGWWSDAYQYSGEG
                     YDENYEWKIRYTPHDANGQPTESTDKMEYKYDVDLESGTTGNMLELSTDQDITLTNID
                     EFNAETGNLIINGSNNLSWKMAYSYELYSYSYEYVHKYKYNSIAASPTNSYPQSGSVS
                     FTIKSNFEGDEVEGDAESLNYYIEAKITFDGDNTALLEFGGYSFTLNLDSFTITPI"
     gene            8563..9783
                     /locus_tag="PK754_06940"
     CDS             8563..9783
                     /locus_tag="PK754_06940"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNB09275.1"
                     /translation="MRLFLSDWVLPVVSPPIRYGGVVTDQGKIIAVGEASTLQDKFPS
                     IVVERMTNTVIMPGLVNAHTHIELSMMKGAIPDGLPFVEWVYRAITGRFTYTTEAIRG
                     AASLAVQSMIQNGTVAVGDIANNSDVSQPLLENSNMWAVIFHEATGFQSALALEKFNE
                     FSRKAWQPGTERVRHALAPHAPYSVSRALFSEIRAFNRSGRLTTMHLAESHDEVVFIR
                     TGEGPMREMIEKLGRWDDTWEAPKTTPVKYAHALGMLMPGMLAVHVVHADDEDIQLMK
                     QFHISVCSCPRSNVKINVGGTAPIRKYLDAGINVCIGTDSLASNDDLNVWNEMHALQK
                     QHASVKPEEIVRIATFNGAKALSLDNVIGTIETGKADALIAVTSRESVSEPFGFLTGG
                     KSTFDSVRPLFAHS"
     gene            9835..10854
                     /gene="aroF"
                     /locus_tag="PK754_06945"
     CDS             9835..10854
                     /gene="aroF"
                     /locus_tag="PK754_06945"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNB09276.1"
                     /translation="MMIVVMHRDASVSDISTVLHIIEESDCTAHVSKAEEKTVVGVVG
                     DDRGIVSEKIQTLQGVEKIFATSKPYKLASREWKKENTLVRVGQVTFGGSDVVLIAGP
                     CGVESEQQILEIAQAVKEAGAHILRGGAFKPRTSPYSFQGLGERGLELLALAREKTGM
                     PIVTEVVTPNQVELVAKYADMLQIGARNSLNYSLLQEVGKTNKPVLLKRGMMSHLEEY
                     MMSAEYILANGNYNVVLCERGIRTFETSTRNTMDIAAIPVLKSWTHLPVIADPSHATG
                     KRSLVQPVAKAAVAAGADGLMIEVHHHPEHALSDGPQAVLPDEYAALVRQLRQISSVL
                     GRSIA"
BASE COUNT         3075 a         2250 c         2736 g         2999 t
ORIGIN      
        1 attacaccgc cattttggca aacgtggtgg tttcgcctgc tgatgttagg gtttgccggt
       61 ttgatcgtgt ggggtatgct taagcttcga tcatattatg cagagtatcg caagacacat
      121 tatgtcagtc attataagtt gcttgaaaaa atcggcgaag gtggaatggg tgtcgtgtac
      181 aaagcgaggg accgcgtatc gggaaagacg gtagctctga agttacttca cgaaagtatt
      241 gcggatacca aagacggatt gcaacgtttt ttaaaagaag cggaagtggg taaattgata
      301 caccatccta atgtcgtgaa aatatacgga gcgggatcga gcgaaacgcg tcggttcatc
      361 acgatggaaa tggtggaagg caagacactg aagcaaacga tacgcgagcg gggagttttt
      421 acgaaagacg acaccaagaa aattattatt cagttagttg aaggtttagc gcaaattcat
      481 gtacacggtg tggtgcatcg tgatttgaaa tcggataata tcattatcaa accggacgga
      541 agggtagtga tcatggattt cggattagca aggatcaagg gaatgacatc ggtagcggcg
      601 agagaccagc ttgtgggtac actggcctat atgagcccgg agcaaacgat cggcaaagaa
      661 gtagatcatc gctccgatat ctacagcctt ggtgtgataa cctatgaaat gctgtacggt
      721 gaaatgcctt ttatagccaa taatgaaatg gaattgatct tggccatcca taatgaaacg
      781 ccgaaacata ttgatgcaaa caatgtagat gcgttgcatc agtgtataaa aatatgtctg
      841 tccaaagatc cgattgatcg ttatccggat acaactacac ttagaaaatt tttagacacg
      901 gtgtgagtta cttttgatgt tccgaaaatt attacatggc tacttatttc ttcttctaac
      961 ctgctttcgc ttgtcttctg cgcaatacca attgcctttt gttcactaca cggcggataa
     1021 tcagatcaat cctcttcccg gtgtagctgt ctatgatgtt tatcaggacc gtttaaattt
     1081 tctctggata agtatttttt cagcaggatt agtgcggtat gacggacacc gcatggaggt
     1141 atataccacg cgcgacggat taagatcgct caattttaca accactatcg aggacacaga
     1201 cggacgtttg tgggttatga ccgatggagg tctttccgtt tcagaacaac ctctgaaaac
     1261 ctatggtgcg catcgcgttc gatttattga tcgcatcggt gatcagccat tggccaaaac
     1321 ttcgatcagt cagattgcga tgaaagtttt agcgcaagat actgcaggcc gaatttacct
     1381 tggcacaacc ggcgcgggta ttttaagata ttcacgggta aaacctgatt tctggagctg
     1441 tgatacgatc accacgcaaa atagccattc atcgcgcaac gcttccgtgt attctatgat
     1501 ggtggataaa aaaggcttag tgtggattag taccgacgac ggcgttgcta ttttaaagcc
     1561 gggcggtcat cgcatagaat atttggaacg ttcgattata cctccggtta gagttcaaac
     1621 attttttcaa acgaatgatg aaactttatt ggccggttgc gaagatggtt cgatctatca
     1681 atgggaaaaa gaggctcatg ggtttcgtaa gatggatcat actttgacca gtccggtata
     1741 tgacattacg caagatcgtc gcggcgattt atggatttgt tcggatggcg ccggtgtgtt
     1801 gcgctggaga aaagggaact caccggaaca catcacacgc cgtcaggggt taattgctga
     1861 aagtgtacga tttgtaatgg aggaccggga aggtacgttg tggttttctc aggttggagg
     1921 attatccaaa ctgagagcaa attataaggc atttcaaagt atttcggcgg atgccacgtt
     1981 ggaaacaggc gtcgtgttgc ctgatccgaa tgtcaccggc atttctaccg aagcatggac
     2041 cggcgataaa cctctccttt ggatgggatt aggtaaaggt tttgcggtat tgacccatga
     2101 tggaaaaata tcgcatttcg atgcaaaaaa aggtttatca agtaatacgg tttacgatgt
     2161 attggtggat gaaaaaaaac gactttgggt aggcacgttc gcaggactta acagttttac
     2221 attctacggt acaccgccag ttactttagg ggccacatct tcgcgacgag ttcaattgaa
     2281 caatcaaccg gcagttatgg attttcatga tattggtatt acgggcgtag tctcgcgtta
     2341 tgcgatgagg gaaaacccgg aggcacaacg tacggttgaa agtctttggt tcaacagcat
     2401 tcgccgactc agtgtctatg tcggtaattc gtggtatatt tttgatgaag cctccggttt
     2461 accatccagt atcatttata gcattgcgtg ggatagtgcg ggttggatgt atgtcggaac
     2521 gggcgatcaa ggaatatacc gcagccgttt ttctttaacg tcgtcagagt taagaaaaca
     2581 attcgaacga tccggtaaag gtttatacca acgcgaaatc accacgccgt gttttgaaaa
     2641 atactggaat caggagaacg gcgctccgtt tgatgatccg caatcacttt tgtttgttgg
     2701 tgatcgcttg tgggtaggtt ctgcgtcgcg cgggcttgtc gtgatcaatc cacaaacacg
     2761 tacccttatt catcaaatga attccgatgc cggttttcga agcccgggca ttatcaattt
     2821 aattttttct ccggttttcg gcaccgtttg gtgcggctcc tccaacggta tatacgaaat
     2881 agatccgata agttataagt tgcttcgcag cgtttttcgc gacgatggtt taataagtag
     2941 cgaggtaaat tggttaacct ccacggcttt tgatagcgat gggaaattat atattggtac
     3001 gcctaaaggt atgacgatct atgatcccaa attagaccgt ccagatctta cttcgccgca
     3061 accgtctatt acttcggcta catttcatca gtcattaggc ggagaaaatg agttgttaac
     3121 tgaattttcc gctttgagtt ttaccaatga aaaagcggtg caatataaag tacggttggt
     3181 agggtatgaa agcgattggc atgcggtatc ggaaccgaga acacgatata cgaatttgcc
     3241 ggcttggttt accgaggcgc gttatcgttt cgaagtgctt gcttccaacg ctcatggcgt
     3301 ttggtctgaa atgcctgctg tttatgaatt taatgtcatg ccggtatggt ggctgcgctg
     3361 gtgggcattt gggatttata ttgcgttgtt cagtgcgttg gttttgggtg cgcgatggtt
     3421 tgttattaat tggcgatccg tgctgatgcc caaaacgcgg tacattgcgc attacaagat
     3481 tttagagaaa atcggccgcg gcggtatggg gatggtgtat aaagcgcggg atacgctaaa
     3541 taaacgaacg gtggccatta aaattctgca tgaagaattt gaacagacgg aggatggtat
     3601 caaacgtttt atcaaagaag ccgaaatagg acgtaaacta aagcatcctc atattgtcga
     3661 aatttatgat gccgggcgat ttgataaaac gcgatatatc acaatggagt ttatcgaagg
     3721 cgttacgcta aaaaattttt tacgagtgca cggacctttg atgccggcgg atgtcgtacg
     3781 aattatccgt ccggtcgcac aagctttgcg aactattcac gcccatgatg tgatacatcg
     3841 cgacctcaag tcggataatg tgatgatacg taacgacggg gttgttaaaa tcatggattt
     3901 tggtttggcc cgcaccaaag gtatgacgac cgtcgtacag cgcgatcagc ttgtcggcac
     3961 gcttgcatac atgagtccgg agcagagcct cggtaaaccg gtggatgcgc gaagcgatat
     4021 ttatgcgttg ggtgtaatca tgtttgaaat ggtggaaggg tgtttaccgt ttaacggtca
     4081 acacgagatg gaattgattt atgccattca caatgaaacg ccggagtttc acaatacgaa
     4141 tattcctgaa gctttgcgtc aatttatatt gcgttgcctg gaaaaagaac ctgagcgccg
     4201 attagccaat atggatgaat tggaagaagt attgaataag atcgctgtat aaatcccttg
     4261 cgaagcgggc tatttctttt aaattaaaat cataacactt acggttcctc acgggtatat
     4321 gatcaaaatt tatgaaagcc tgcaggattt gcaggtcaaa atagacgaaa cattcaaaga
     4381 gctatcgcag ggatttaata aagtcaacgg cgcgatcacg caaaaaaacc ttcccgccgc
     4441 cgaatccgca ctcaatgatc tttttaaagc gtttatgtca cgtacccgtg cgatcaacgc
     4501 ccagtcggcg tcggtcggcg cggacatcaa aaaaatagaa tcccagtgcg ctgaaatatc
     4561 cgccgaaaaa gaacaattca aagcgttgta taattccggt attgcgatga caacgcaagg
     4621 cgaattgcag ggactgatca catttgcgat gaatcagatg acttatcatc ttcgcgcgga
     4681 gcgaggtttt ctgattttgg tggatgatca cggtgagcgc gaatattttg tcagtaaaaa
     4741 ttttgacgga caaaatattg acgatccggc cgccgaagtg agcaccagcg taattcaaaa
     4801 aacattggat gcgttacaac cgatcaaggt ggacgacgat acactatccg agagcatcat
     4861 gaaacaaggt agttttgtgc gtctcgggct tcgtgccgtg atgtcagtgc cgatcatttt
     4921 cagaaaaaaa ttactcggtg tggtttatct tgaccggcgg gaagaaacca aaggtttttc
     4981 ggataacgac ttggcttttc tgatggcatt ttcccggcag attgcttttc gggttaatga
     5041 actcaaggaa caacagcatg ttcataccga atacgaacgc cgtgatcgta atcgtttaca
     5101 atcgttgcga gaaaaatata atttttctga aatcatcggc aaaagcgaac ggttggtcaa
     5161 agtactcgaa ttggccggta aagtagcatc ctccgaggtt actgttttga tccaaggcga
     5221 gagcggtacg ggtaaagaat tgatcgctcg tgccattcat tttaacagcg accggtacga
     5281 acataaattc atagctatca attgcggtgc gattccctcg gaacttcttg aatcggaatt
     5341 attcggatac gaatcgggcg catttaccgg cgccgtcaaa accaaaatcg gaaaatttga
     5401 agcggcgcat cgcggaacct tgtttttgga tgaagtggcc gagttgtcta tgaatcttca
     5461 ggccaaaatt ttgcgtgttc tgcaaaccaa agaactcgaa cgtcttggca gcacggaaac
     5521 gcgcaaaatc gacctgcgca tcatatcggc cagcaataaa aatctccagc agatggtgcg
     5581 ggaagggaaa tttcgtgaag atttgtatta ccgtctcaaa gtcgtagaga tcgaattacc
     5641 ggcgttacgc gaacggaagg aagatattca tcttctggtt agtttttttg ttcagaaata
     5701 cggtgaacag cgaattcagt cggtcagcga agatgcgatg gatattttag aaaactacgc
     5761 ctggcacggc aatatccgtg aattggaaaa tgtcattcaa cgtgcgatca ttctttcgtc
     5821 cggcacgcag atcgaagtgc aggatttacc tccggagatt gtttccaaaa cggaaagcgg
     5881 atataaggta gacaaagatt tgtcactcga agaagcggaa acggattttc ggcgatggtt
     5941 tgtgttacgt gccctacgga aaaataacaa caataaatcc aaagccgctg aaatgctcgg
     6001 tatcaatcgt actcactttt tccgaatgct caatcagctc ggcatttcag atacagaata
     6061 gcagcttttt cagattttcg ttgccctctc ccaaccctgc ccctgagttt taagtagctt
     6121 ttttgcgacc atttacgcat ttcggtctgt cgtattattg taacacattt tacgatttac
     6181 gacagggcgg gattgatttt tttaatgtta atttttggca cagcaattga tattcctata
     6241 ataccattat cagggatttc atagtgaaaa aaaaggttct catttttaat caagacattg
     6301 atgaattatc ggtcatacgt caggtattgg ttcgggaagg gtatgaagcg ataacggcat
     6361 ccaattggga tacggcgact aaacttttcg ccagccttga gattgactat gtgcttatga
     6421 atggtaaatc cagcgatatt cagcgcgttt tacaaaacgc actcaagatg tgatcaagta
     6481 ttcgtactgc agctttaaaa agttgctcgt gcgtgaggac cgcgcggttc taatgggccg
     6541 cgcggatttt ttgaaaagta tgttggatat catagacaaa cgtaaagccg taaggtaaat
     6601 ttcgatcata agtaatcatt aaaatcaatg ggagggtttt atgaaacatc taatgacatg
     6661 ggtttatatt ttggtgttgg ttgcgttgac ttcatgtggt ggcgatgatg cggccaaacc
     6721 ggaagaattc ggaacgcagg aagaagccct tacggcgctg aatgcaaata aatcgaatat
     6781 gttgctggct ttggatgccg cggaaggagc gattgatgcg gcttcaggtt ttggattaga
     6841 caaacgttcc gcgtataatt ccaagaaact taaccgtgca atgacttata cgtacgacgc
     6901 atctacgggt tggtggacgg acgagtatac ctattcatcc ggcgggtata attatgatta
     6961 cacatggaac attcgtttta caccgcgtga tgctaatggg tattcgacgg atgaaacttc
     7021 aaaaatggaa tataaatacg attaccttta taacggggca aatacatatt attcatatga
     7081 catggatcta ggtgccgata tgacgttgac cggagtcaat gcgtatcgtg cagcgaccgg
     7141 aaatcttgta ttaaatggta cgaatgcgat cagttatgta tgggattatt catacctcac
     7201 gtatgattac acgtatgatt acagtcataa atatacctat aagtctctag agatatcacc
     7261 cgatggtgat tatcccgaag gcggctcggt cagtttcacc attcgttcca agtttgatgg
     7321 tgagataggt gaagaaggcg gtgatgcatt gaattattat gtcgctggaa aaataacgtt
     7381 cgacggagac aatacggcgt tgctcgaatt cggcggtttt tcttttattc ttaatctgga
     7441 taccaatgaa gtaacacctg cgtaaaaatc ccctttctta ttggattaca accaaaccgc
     7501 ttcggttcgt caagagccgg gcggtttttt ttatgtagaa ttttattggc ttcaggcaac
     7561 tcttgtcgta ccttgcccat atagccataa aaacaaggga ggtaattatg aaaaacgtaa
     7621 tgcgattttt ggcagtagcc atgtccacgt ttatgatatc gtgcggcggt gacgatgaac
     7681 tcaaacctga aaaatttgca acccaagaag aagccatgac ggcattttcc aataatcagt
     7741 acaacctgca agacgcttat catagcgttg gcgatgcgat tgattataca ggcggtgtga
     7801 gtgtaggtgc ggttaaaact gaacaaaaga aaaaaatgaa ccgcgccatg acttgggctt
     7861 acgacgcatc caccggctgg tggtcggatg catatcaata tagcggtgaa ggctatgacg
     7921 aaaactatga atggaaaata agatacactc ctcacgacgc caatggtcaa ccgaccgaat
     7981 ctacagataa gatggagtat aaatacgatg tagatttaga gtccggcacg accggtaata
     8041 tgctcgaact ttcgacggat caagatataa cgctgactaa tattgatgaa tttaatgctg
     8101 aaaccggaaa tctgattatc aacggttcaa ataatttatc ttggaaaatg gcttattcgt
     8161 acgaactgta ttcgtattca tacgaatacg ttcacaaata taagtacaat tcgatcgccg
     8221 catccccgac caattcgtat ccgcaaagcg gttcggttag ttttacgatc aaatcgaatt
     8281 ttgaaggaga tgaggtggaa ggtgacgccg aatcgcttaa ttattacatt gaagctaaga
     8341 tcacttttga tggtgataac acggcgctat tagaatttgg cggatacagt tttacgctca
     8401 atttggatag ctttacgatt acaccgatct gatttggatt tcatattttt tcgaataacc
     8461 gcttcggttc gcacagagcc gggcggtttt ttattttgta tcaaagaatc atgagatgta
     8521 gtaaattcta cattttgaga gaaaaaagga gatcggcgtt ttatgcgttt atttttgtct
     8581 gactgggtat tacccgtggt gtctccaccg attcgctatg gcggcgttgt tacagatcaa
     8641 ggaaaaataa tcgccgtagg tgaagcttct acacttcaag ataaatttcc atccatcgtc
     8701 gtggagcgga tgacaaatac ggtaatcatg ccgggtttgg tcaatgcaca cacccacatc
     8761 gaactgtcaa tgatgaaagg tgctattccc gatgggctcc catttgtaga atgggtgtat
     8821 cgtgctatca cgggacgttt tacttataca actgaagcta ttcggggcgc agcatcacta
     8881 gctgtacaaa gtatgatcca aaacggtact gtggctgtgg gcgatattgc caataattcc
     8941 gatgtatcgc agcctctttt ggaaaattcc aatatgtggg ctgttatctt tcacgaagca
     9001 accggatttc aatcagcctt ggcgcttgaa aaatttaacg aattttcccg taaagcatgg
     9061 cagcccggca ccgaacgcgt gcgtcatgcg ttggccccgc atgcgccata ctcagtatca
     9121 cgcgcactat tttccgaaat tcgcgcattc aatcgtagtg gccgtctgac aacgatgcat
     9181 ttggcagaat cgcacgatga ggtggtcttt atacgaaccg gcgagggtcc gatgcgggaa
     9241 atgatcgaaa aactcgggcg gtgggatgat acgtgggaag cgcccaaaac cacacccgta
     9301 aaatacgccc acgctttggg tatgctgatg ccgggtatgc tggccgtgca tgtagtgcac
     9361 gcggatgatg aagatattca actgatgaaa cagtttcaca tctcggtatg ctcatgcccg
     9421 cgaagcaatg taaaaatcaa tgtcggcgga acggcaccga tcagaaaata tctcgatgcg
     9481 ggtatcaatg tatgcatcgg cacggacagt ttggcaagta atgatgatct gaatgtgtgg
     9541 aacgaaatgc atgctttgca aaaacagcat gcctctgtga aaccagaaga aatagttcgt
     9601 atcgctacgt tcaacggcgc caaagcccta agtcttgata atgtgatcgg cacgatcgaa
     9661 accgggaaag ccgacgccct cattgcggtg acttcacgcg aatccgtgtc ggaaccgttc
     9721 ggtttcctga ccggcggaaa atcgacgttt gattcagtac ggccgttgtt cgcccattct
     9781 tgatcggtgt attttttttg cgtagattgt gacaatcgaa aggtgatttt atttatgatg
     9841 attgtagtga tgcatcgcga tgcatcggtt tcggatattt cgaccgtcct tcatattatc
     9901 gaagagagcg attgcacggc gcatgtcagc aaagcggaag aaaaaaccgt cgtcggtgtc
     9961 gttggcgatg atcgcggtat cgtgagtgaa aaaattcaga ccttacaagg tgttgaaaaa
    10021 atatttgcga catctaaacc gtacaaattg gccagccgtg agtggaaaaa agaaaataca
    10081 ctggtacgtg taggacaggt tacgttcggc ggttcggacg tcgtacttat cgccggtccc
    10141 tgtggcgtcg aaagcgagca gcaaatttta gaaatagcgc aggccgtcaa agaagccggg
    10201 gcgcacattt tgcgcggagg tgcgtttaag ccccgcactt cgccttatag cttccaagga
    10261 ttaggtgaac gcggtctcga attgcttgct ttggcacgcg aaaaaaccgg tatgccgatc
    10321 gtgactgaag tggtgacgcc caatcaggtc gaactcgtcg ctaaatatgc cgatatgcta
    10381 cagatcggcg cacgcaattc gcttaattat tcacttctgc aagaagtcgg caaaactaat
    10441 aagcctgtat tgctcaaacg cggcatgatg tcgcatctgg aagaatatat gatgtcggcc
    10501 gaatacattt tagccaacgg taattataac gtagtattgt gtgaacgcgg aattcgtacg
    10561 tttgaaacgt ccacacgcaa tacgatggat attgctgcaa ttcccgtact taagtcatgg
    10621 acgcatcttc cggtgatagc cgatccgagt catgccaccg gcaaacgttc gctggtacaa
    10681 ccggtagcca aagcggccgt cgcggccgga gcggacggcc ttatgatcga ggtgcaccat
    10741 catcccgaac atgccttatc ggacggtccg caagccgtac tgcctgatga atacgccgca
    10801 ttggtccgcc aattgcgaca aatcagctcc gtactcggac gctcgatcgc ctaaacgaaa
    10861 tttatttcat aaccgtttgt tcatgaaagt atccaccgtg acgacgcgtt attcctatct
    10921 tgaagaatct tcattgtcga gtgaaaaact cgtggacggc catctgctca aggcctggcg
    10981 cgatgtcgta cagttgcccg acggcaaaac aggcattcgc gaatatattc gtcaccccgg
    11041 cgcggtgatc atgctgccgt
//
LOCUS       DAOQNQ010000083        10961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2133_length_10961_cov_5.272786, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000083 DAOQNQ010000000
VERSION     DAOQNQ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10961
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2133_length_10961_cov_5.272786"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="PK754_06950"
     CDS             complement(<1..776)
                     /locus_tag="PK754_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB09277.1"
                     /translation="MALLRRMILTLFYPLAFLLASNNGGNPLVDSLSSISPESPYYST
                     AMVRLSEILMTENTVLSRRAAEEAMRNAIRTSPDDPWHYVRLSKIQFERSFYRSAFAA
                     CDDAIRINQSKPVPYHDVFSEAYYYKGLIKERQALKYKDMISPVNEDFSISLSNFGYE
                     DMLESARYYEKALEYNGTHRDALFHLGLLYYEIRAFDKMSALFEKIIQYKPEDKDALM
                     FLGLAYYHRGDFGQSHTYYQRAIHAMSPEEASRVNHIGHIV"
     gene            complement(876..1355)
                     /locus_tag="PK754_06955"
     CDS             complement(876..1355)
                     /locus_tag="PK754_06955"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNB09278.1"
                     /translation="MITDFFRRRLIQPLMDLLRQGLSAEKLALSVSLGFVLGIFPMIG
                     ATTILCLVIGALFRVNQLAIQTVNYFLFPVQIAFYIPFFQMGAWLFGYDHFPFTFEEI
                     ATMLSRNPWQAIVSLWWANLRAITAWLLTAPLLGGILYLILVPVFKPITARFFPDKA"
     gene            complement(1352..2623)
                     /locus_tag="PK754_06960"
     CDS             complement(1352..2623)
                     /locus_tag="PK754_06960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNB09279.1"
                     /translation="MQEVMSTPLPQSADNTKKIMPGFYEKLVLRTFEGMHQGYMRIHL
                     PDGRMYTYGNANAELQATIQVRSNNFFKKCALYGDIGFGEAYVDGDWDTDSVTRVIRW
                     FLLNVEQAPTISGSKRRIVPANFLKGINRLYHRLRENTVRLARMNIQAHYDLSNEFFK
                     TFLDSSMTYSSAYFRTPDMSLEAAQTEKYDRLCRQLKLKPTDHVLEIGSGWGGFALHA
                     VKHYGCKITTITISEEQYHYARARFAAEGFADRISILFTDYRNVSGQFDKIVSIEMLE
                     AVGHRYFKTYFAKLHEVLKPNGLVGLQVIICPDSRYAALRKGVDWIQKHIFPGSLLPS
                     VGVLHEAAHRTGDLSLVDLKHLGRHYARTLATWRDRFNQNNELVQALGFDESFRRKWN
                     YYLSYCEAAFESRNIGVVQMIWSRPNNMLVS"
     gene            complement(2580..3383)
                     /locus_tag="PK754_06965"
     CDS             complement(2580..3383)
                     /locus_tag="PK754_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HNB09280.1"
                     /translation="MNSCLYRCRVMHHRMEPKPHRFFYNIFMFYLDLDELDTLSDKLT
                     LFGHNAFAVFNFKDTDHLFYSGTSRREHITTYLQQNGITSPVGRIMLLTHVRMLGYVF
                     NPVSFYFIFDNDEQPICAIPEVGNTFGEMKPYLIRHSTESPHTFYRKTTKFFYVSPFI
                     DIDAEFEFDLTLPDDQLMMRINDIQNNRRFFVSTLSGKRQILTDARLALYLLRFPLVT
                     LQVIFFIHWQAFRLWLKKLHYHKKTDFPDLQREVHHAGSYVNAIAAERR"
     gene            complement(3380..4645)
                     /locus_tag="PK754_06970"
     CDS             complement(3380..4645)
                     /locus_tag="PK754_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB09281.1"
                     /translation="MERIAIIGTGIAGMGCAHFLNHRYDITMYEQNNYIGGHTHTITV
                     HEDGSPVFIDTGFMVFNLVTYPNLVRLFKELGVDYKPTSMSFSVQHVPSRLEFNGSGI
                     NELFAQRRNLLNPSYIRMLLEINRFNNTCREVIEKSQFADYTLKEYVEEKGYSDDFLM
                     KYLVPMSSAVWSTPPDAMLGFPAAALIRFFDNHGFLGLHTQHPWYTPVNGSQQYREKL
                     IAPFRDRIFTDRAAVHVRRENGKVFITDKRGDKAAFDKVIIATHADQALAMLEQPSEA
                     EYRLLKEFKYQKNITTLHTDASGMPRTRRAWASWNYRIDQKHDGSLEAHTIYWMNSLQ
                     HVSQKTNYFVSINGAERIAPDRVIRRMTYEHPVFSVAAMKAQSELPTLNIKEPVYFCG
                     SYFRYGFHEDAFMSALALCRQITGEAIWL"
     gene            complement(4669..5394)
                     /locus_tag="PK754_06975"
     CDS             complement(4669..5394)
                     /locus_tag="PK754_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09282.1"
                     /translation="MNLKRSYYLLLTLSSVSLSAIVSPTETPTLQLKTLAWDNKSGAL
                     LYTEERKQFDFDGKPGRWEFNYQNPKGQIVVQRSVNFQQDRLKPSFILRDLRNGYSEG
                     AETIGNKIKVFSGGSPDDPYQEKTIRVPEPAVIDAGFHFFIEQHWSALLNGETKTFYF
                     VAPSQLDYFSFRVYKSGETQNGGRTAYQLTMDIDNFLLRLFVDPIRLTYDPESRKLIG
                     YEGISNIYNDEGKAHHVRMEFVY"
     gene            complement(5391..6419)
                     /locus_tag="PK754_06980"
     CDS             complement(5391..6419)
                     /locus_tag="PK754_06980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNB09283.1"
                     /translation="MIHTLLKNNLIPDAFIRYGIRRLLRQRLFEEKKPTIELQWQHWK
                     NYINMLKESPIAIDTKAANEQHYEVPTRFFQLVLGRHLKYSSGYWHEGVTELDRAEND
                     MLALTCSRADLHDGQDILELGCGWGSLSLYMAEHFPQSRITAVSNSRTQKEFIDATAR
                     QRGFSNLTIITADMNHFTTDQKFDRVVSVEMFEHMRNYRALLAKVARFMKDDARLFVH
                     IFTHREYAYLFEVRDESDWMAKYFFTGGQMPGDMLLHYFNEDLRIEEHWRVSGLHYAK
                     TSEAWLANMDRQRAEIIPLLEATYGTNNARMWWVYWRIFFMACAELWQYNQGDEWLVS
                     HYRFRKNI"
     gene            complement(6441..7220)
                     /locus_tag="PK754_06985"
     CDS             complement(6441..7220)
                     /locus_tag="PK754_06985"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNB09284.1"
                     /translation="MVHDFWNYQLIALIVMILIMTIVWIIHLIIRRADIVDIAWAYSL
                     AILAGMFALLSPYEGLRPVLMAALVGVWGFRLGTHLLLRIAGHEEDGRYQQMRREGKG
                     ALEFFWFFQFQALLNVILCIPFSFVALNPVNRLGFFEYAGLSIGVIAIIGESFADAQL
                     RRFKARTDTRGQVCRDGLWKYSRHPNYFFEWLIWVAIFVFACGSPLGWISVIGPLLML
                     YFLFKVTGIPATEAQARRSKGLAYAEYQRTTSAFVPWVNKQ"
     gene            complement(7236..7586)
                     /locus_tag="PK754_06990"
     CDS             complement(7236..7586)
                     /locus_tag="PK754_06990"
                     /inference="COORDINATES: protein motif:HMM:NF018991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1475 family protein"
                     /protein_id="HNB09285.1"
                     /translation="MLTFLKILFSVIFAGMIAVVVTTSYESNLFTLIATWDSANTMAP
                     WFSATLWDFYANVVFIFVWIAYKENNVFRSIVWLILLVALGSIATALYALIQLFKLQP
                     GQNIRDFFTAQNAA"
     gene            complement(7597..8343)
                     /locus_tag="PK754_06995"
     CDS             complement(7597..8343)
                     /locus_tag="PK754_06995"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNB09286.1"
                     /translation="MEIAIFFAGHWFTSLFSQSFFLHRYSAHKMFNMNAFWERFFYFF
                     TFLTQGSSFLNPRAYAVMHRMHHAFSDTEKDPHSPHFFKDVFRLMWHTKKVYFDLLTG
                     KTIPEQQFDGDTPRWGWLDAMSNAWVVRVAWGVGYFIFYYFFVPEGYTWLYALLPLHF
                     LMGPIHGAIVNWCGHKYGYANFDNRDKSKNSLVWDFMLLGELFQNNHHKYPNRPNFAV
                     RWFELDPTYPIMKVLDWMRIIRIRRKSNAA"
     gene            8841..10664
                     /gene="typA"
                     /locus_tag="PK754_07000"
     CDS             8841..10664
                     /gene="typA"
                     /locus_tag="PK754_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNB09287.1"
                     /translation="MKRRTDIRNVAIIAHVDHGKTTLVDQLLRQSGTFRENQQIAERV
                     MDSNDLERERGITILAKNTSIRYKGAKINIVDTPGHADFGGEVERIMTMVDGVLLLVD
                     AFDGPMPQTKFVLKKALASNCTPIVVINKIDRPGARPHEVLDEVFNLFLELNANDEQL
                     DFATIYASAKMGFAQYEIDQANSDMVPLFDTILERIPSPPGEREAAFQMLVANIDYSD
                     YLGRIAIGRIQRGEIKTGQNVAVMKKDGTRYNSRVVKLFAYEGLKRTETESAYSGEIV
                     AMAGMEEIQIGETIADYNNPEALEPIHVDEPTIAMNFIVNNSPFAGQDGKFVTSRHLR
                     ERLYKELLSNLALRVEDTESMDTFKVSGRGELHLCILIENMRREGFEFQVSRPEVIMK
                     RVDGQLLEPMEYLIIDVPEEYMGVVIEKLGKRKAEMKNLIHLDNKQVKIEFVIPARGL
                     IGYRGEFLTDTRGTGVLNHIFHDYAPYKGDIGGRLNGVLISMEQTETVAYALFGLQER
                     GELIVDPQTPVYEGMIIGKHSRENDLVVNVGKGKKLTNMRASGSDDAVRLEPVRPMSL
                     EDYIEFINDDELVEITPKTFRLRKKVLKESDRRREERKKKD"
     gene            10687..>10961
                     /locus_tag="PK754_07005"
     CDS             10687..>10961
                     /locus_tag="PK754_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HNB09288.1"
                     /translation="MFNDLSAKIEGAFKRLAGRGVINEKNIQEALREIRMALLEADVN
                     FKVVKDFIDKATAKALGQEVLRSVSPGDQLVKIVYDELVETLGHEHAP"
BASE COUNT         3182 a         2399 c         2547 g         2833 t
ORIGIN      
        1 acgatatgtc cgatatgatt aacacgcgaa gcttcttccg gcgacatcgc atggatggcg
       61 cgttggtagt aggtgtggct ttgtccaaaa tcgccgcggt gataataagc cagccccaaa
      121 aacatcaagg catctttatc ctccggctta tattgaataa ttttttcaaa caaagccgac
      181 attttatcaa acgcacggat ttcataatat aacaacccca gatgaaaaag ggcatcccga
      241 tgtgtgccat tatattccag cgctttttcg tagtatcgcg ctgattccaa catatcttcg
      301 taaccgaaat tggataaaga aatgctgaaa tcttcgttaa cggggctgat catatctttg
      361 tatttgagcg cttgccgttc tttgatcagg cctttgtagt aataggcttc ggaaaaaaca
      421 tcatgataag gcactggttt agattgattg atccggatcg catcatcaca ggcggcaaac
      481 gcagaacggt aaaaactgcg ttcaaactgt attttgctta aacgtacata gtgccacgga
      541 tcgtcgggtg atgtgcgtat agcattgcgc atagcctctt ccgcggcacg tcgggatagc
      601 acagtgtttt ccgtcatgag aatttcagac aggcgtacca tagccgtgga atagtatggt
      661 gattcgggcg aaatgctcga tagagaatct acaaggggat tcccgccgtt attactcgcc
      721 agtaaaaaag ctagcggata gaacaacgta aggatcatcc tacgcagcag tgccatgcgt
      781 ctaaagcttt caaaaaaagg atatgatact acaacgaaaa acgtaatcag gttattccgg
      841 caatccgatg aagatacatt gttgggtttt ggaattcaag ccttgtccgg aaagaaacgc
      901 gctgtgatag gtttgaaaac gggaactaaa atcagataga gtattccgcc taaaagcggc
      961 gccgtaagaa gccatgccgt tatagcacgc agattggccc accataggga tacgatggct
     1021 tgccatggat tacggctgag catggttgcg atctcttcaa acgtgaaagg aaaatgatcg
     1081 tatccgaaaa gccaagcacc catctgaaaa aaaggaatat aaaaagcgat ttgtacagga
     1141 aagagaaaat aattaaccgt ttgaatggcc agttggttaa cacgaaaaag cgctccgatc
     1201 accaaacata aaatggtcgt ggcgccgatc atagggaata tacccaacac aaaaccgagg
     1261 ctgacactca acgcaagttt ttcggcggaa aggccttggc gtaacaaatc catcagcggc
     1321 tgaataaggc gtcgtcgaaa gaaatcggtg atcatgaaac caacatatta ttggggcgtg
     1381 accatatcat ttgtacaaca cctatattac gcgattcgaa agccgcttcg caatagctaa
     1441 gataataatt ccatttccgt cggaaacttt catcaaaacc cagcgcctgt accagttcgt
     1501 tattctgatt aaagcgatca cgccaagtgg ccaatgtgcg ggcataatga cgaccaagat
     1561 gtttgagatc aaccagtgaa agatcgccgg tgcgatgagc ggcctcatgg agcacaccta
     1621 cggatggcaa caaggatccg ggaaaaatat gtttttgaat ccaatccaca cctttgcgca
     1681 acgcggcgta gcgcgaatcg gggcatataa tgacttgcaa gccgacaagg ccattgggtt
     1741 ttagcacttc atgaagtttt gcaaagtaag ttttgaaata acgatgccct acggcctcca
     1801 gcatttcgat ggatacgatt ttgtcaaatt ggccgctgac attgcgatag tccgtaaaca
     1861 atattgaaat ccgatccgca aatccctcgg ccgcaaaacg tgcgcgggca taatgatact
     1921 gctcttctga aatcgtaatc gtcgtgattt tacaaccgta atgtttgacc gcatgcaagg
     1981 caaacccgcc ccagccgcta ccgatttcga ggacgtgatc cgtcggcttg agtttcagtt
     2041 gacggcaaag gcggtcgtat ttttccgttt gcgcggcttc cagcgacata tcgggcgtac
     2101 ggaaatacgc gcttgaataa gtcatggaag aatccagaaa tgttttaaaa aattcattgc
     2161 tcagatcgta atgagcctga atattcatac gcgcaagcct gacggtattt tctcgtaacc
     2221 gatgatataa acggttaatc cctttaagaa aatttgcggg gacgatgcgg cgcttggatc
     2281 ctgaaatggt aggcgcttgt tcgacgttga gcaaaaacca gcgaatcaca cgggtaacgc
     2341 tgtcggtgtc ccaatcacca tccacatagg cttcgccgaa accgatatcg ccgtagagag
     2401 cgcatttctt aaaaaaatta ttactgcgca cttggatcgt tgcctgcaat tcggcattgg
     2461 cattaccgta cgtatacatt cgtccgtccg gcaaatggat gcgcatgtaa ccttgatgca
     2521 ttccttcgaa cgtacggagt accagttttt cgtaaaaacc aggcataatt ttttttgtgt
     2581 tatcggcgct ctgcggcaat ggcgttgaca taacttcctg catgatgtac ctcccgttgt
     2641 aagtcgggaa aatccgtttt tttatgataa tgtaattttt ttaaccacag tctgaatgcc
     2701 tgccagtgaa taaaaaatat cacctgcaag gttacgagtg gaaaccgaag aagatacagt
     2761 gccaatcttg catcggttag tatctgtcgt tttccggata aggtgctgac aaaaaaacgg
     2821 cgattattct gaatatcatt gatacgcatc atcagctgat cgtccggcaa cgtgaggtca
     2881 aattcaaact ccgcatctat atcaataaaa ggggagacat aaaaaaattt tgtggttttt
     2941 cggtaaaatg tgtggggcga ttccgtgctg tggcgaatca agtagggttt catttcgcca
     3001 aacgtgtttc cgacctccgg aatggcgcaa atcggttgct cgtcattatc gaaaataaaa
     3061 taaaacgaca cgggattgaa tacgtaacct aacatgcgaa catgcgtcag caacatgata
     3121 cgacctaccg gagaagtaat gccgttttgt tgcagatagg ttgtgatatg ctcacgtcgc
     3181 gatgtgccgc tataaaataa atgatcggtg tccttaaaat tgaaaactgc aaacgcatta
     3241 tgtccgaaaa gtgtaagttt atcggatagt gtgtccaatt cgtccaaatc cagatagaac
     3301 atgaaaatat tatagaaaaa gcgatgcggc ttcggttcca tccggtgatg catgacacgg
     3361 catcggtaca ggcatgaatt cataaccaaa tcgcttctcc cgtaatttga cgacacaatg
     3421 ccaatgcgga cataaacgcg tcttcatgaa atccatagcg aaaataactc ccgcaaaaat
     3481 acaccggttc tttgatgttg agggtcggga gctcggattg cgctttcatc gcggctactg
     3541 aaaaaacggg gtgttcatag gtcatgcggc gaatcacgcg atccggtgca atgcgttccg
     3601 cgccattgat cgaaacaaaa taatttgttt tttgggatac atgctggagc gaattcatcc
     3661 aataaatcgt atgcgcctca agtgaaccgt catgtttttg atctattcga taattccatg
     3721 aagcccaggc ccggcgggtt cgcggcatgc cggaggcatc cgtatgcaat gtcgtaatat
     3781 ttttttggta tttaaattct ttcaataggc gatattcggc ttccgacggt tgctccaaca
     3841 tggccagcgc ttggtcggcg tgcgttgcta taatgacttt atcaaaagca gctttgtcgc
     3901 cgcgtttatc cgtaatgaaa actttcccat tctcgcgacg aacgtgtacg gcggctcgat
     3961 ccgtaaaaat acgatctcta aaaggtgcga tgagcttttc ccgatattgt tgactcccgt
     4021 tgaccggtgt gtaccatgga tgttgggtgt gtaaaccgag aaaaccgtgg ttatcaaaaa
     4081 agcgtatcag cgctgcggcc ggaaaaccaa gcatcgcatc cggcggggta gaccatacgg
     4141 cggaactcat tggtaccaaa tacttcatca aaaaatcgtc actatatcct ttttcctcaa
     4201 cgtattcttt cagcgtatag tcggcaaatt gcgatttttc gattacttca cggcacgtat
     4261 tgttaaaacg gtttatttcc aaaagcatgc gaatataaga cgggtttaat aaatttctac
     4321 gttgcgcaaa taattcgttg atgcccgatc cgttaaattc caatcggctg ggtacatgtt
     4381 gaacactaaa agacatggaa gtaggtttat agtctacgcc cagttcttta aaaagtcgca
     4441 ctaggttggg atacgtaacc aagttaaaaa ccataaagcc ggtatcaata aaaacaggcg
     4501 atccgtcttc atggacggtg atcgtatgag tatgccctcc gatgtagttg ttttgttcgt
     4561 acatcgtaat gtcataccgg tgatttaaaa aatgagcaca tcccattccg gcaatgcctg
     4621 tgcctataat agctatccgt tccaaatcaa cttcgctccg aattgaaatt aataaacaaa
     4681 ttccatgcgt acatgatggg ctttgccttc gtcattatag atattggaaa taccctcgta
     4741 tccgatcagt ttacggcttt ccgggtcata tgtaagtcgt atcggatcca cgaaaaggcg
     4801 gagtaaaaaa ttatcaatat ccatagtcaa ctgatacgca gtgcggcctc cgttctgagt
     4861 ttcaccggat ttataaacgc gaaatgaaaa ataatccaat tggctcggtg caacgaaata
     4921 aaacgttttg gtttcaccgt tcagtaaagc gctccaatgt tgttcgatga aaaaatggaa
     4981 tcctgcatca atcacagccg gttcaggaac ccggattgtt ttttcctgat acggatcgtc
     5041 cggcgaaccg ccgctaaaaa ctttgatttt attaccgatg gtttctgcgc cttcactata
     5101 gccgttgcgt aaatcgcgaa gtataaaaga aggcttgagt ctgtcttgct gaaaatttac
     5161 ggaacgttga acgacgattt gtcctttggg attttggtaa ttaaattccc atcgccccgg
     5221 ttttccgtcg aagtcaaatt gtttacgttc ctccgtgtac aagagagccc cgcttttgtt
     5281 atcccatgcc agggttttaa gttgcagtgt cggcgtttcc gtcggcgata ctatcgccga
     5341 caaactgacc gacgaaagcg taagcaaaag gtaataggat cgtttcaggt tcatatattt
     5401 ttcctgaatc ggtaatgact gactagccat tcgtcgcctt ggttgtactg ccatagttcg
     5461 gcgcaggcca taaaaaaaat tcgccagtac acccaccaca tgcgtgcatt attggtgccg
     5521 taagtagctt ctagaagcgg tatgatttct gcgcgttggc gatccatgtt ggccagccac
     5581 gcttcggatg ttttggcata atgcaaaccc gatacgcgcc aatgttcttc aatacgaagg
     5641 tcctcattga aataatgcaa aagcatatca ccgggcattt gaccgccggt aaaaaaatat
     5701 ttggccatcc aatcgctttc atcgcgtacc tcgaaaagat acgcatattc ccggtgagta
     5761 aagatatgca cgaaaagccg ggcatcgtct ttcataaacc gggcgacctt ggcgagaagc
     5821 gcgcggtaat tgcgcatgtg ttcaaacatt tctacagaca ccacgcgatc aaatttttga
     5881 tcggtcgtaa aatgattcat gtcggcagtg atgatggtca gattactgaa cccgcgttgc
     5941 cgtgctgtag catcaataaa ttccttttgt gtgcgggaat tggaaaccgc ggtgatgcgg
     6001 ctttgaggaa aatgttcggc catataaagc gatagggaac cccaaccgca accgagttcc
     6061 aaaatatcct gtccgtcatg cagatcggct cgactgcaag ttaaggccag catgtcgttt
     6121 tcggcgcgat ccaattccgt gacgccttcg tgccaataac cggaactgta tttcagatgc
     6181 cgtcccagta cgagctgaaa aaaacgcgtt ggcacctcgt aatgttgctc attcgcggcc
     6241 ttggtatcaa tggcgatagg gctttctttg agcatattga tatagttttt ccagtgctgc
     6301 cattgaagtt cgattgtcgg ttttttttct tcaaacaatc gttgacgtaa tagtctgcga
     6361 atgccgtaac gaataaacgc atccgggatg aggttgtttt taagtagggt atgaatcatg
     6421 tcgtagtttt gtatattttg ttattgttta ttgacccacg gtacgaacgc gctggtggtg
     6481 cgctgatact ctgcataagc gaggcctttg gagcgccgtg cttgcgcttc cgtagccggg
     6541 ataccggtga ctttaaaaag aaaatacagc atcaatagag ggccgataac ggagatccac
     6601 cctagcggtg agccacacgc aaaaacaaaa atggccaccc atatcaacca ttcgaaaaaa
     6661 taattagggt ggcgtgaata cttccataat ccatcgcggc agacttggcc gcgtgtgtcg
     6721 gtacgtgcct tgaaacgacg gagttgcgca tccgcgaacg attcgccgat aatcgcaata
     6781 acgccgatgc tcaaacctgc gtattcaaaa aaaccgaggc ggtttacggg gttgagcgca
     6841 acgaatgaaa acggaataca gagtatgaca ttgagcagag cttgaaattg aaaaaaccaa
     6901 aaaaattcca atgctccttt tccttcgcga cgcatttgtt gatagcggcc gtcttcctca
     6961 tgacctgcga tacgcaacaa gaggtgcgta ccaagtcgaa aaccccaaac cccgacaagt
     7021 gcggccatca gaacagggcg caaaccttcg tacggtgata acaaggcaaa cattcccgcc
     7081 aatatcgcca gactgtatgc ccaagcgata tcaacaatat cggcgcgtcg gatgatcaga
     7141 tggataatcc aaacaatcgt cataattaga atcatgacga tcagcgcgat cagctgatag
     7201 ttccaaaaat catgtaccat gcgtcttttc tacgtttatg ctgcgttttg tgccgtaaaa
     7261 aaatcacgta tattttgccc gggctgcagt ttgaaaagct gaatcaatgc gtacagcgcc
     7321 gtggcgatgc ttccgagtgc aaccagcaag atcaaccaca caatactgcg aaaaacattg
     7381 ttttctttat atgcaatcca cacaaaaata aaaacgacat tagcataaaa atcccacagc
     7441 gtagccgaaa accacggggc catagtattc gcggagtccc aggtagcgat caaagtaaac
     7501 aggttacttt cgtagctggt agtaaccacg acggcgatca tgcccgcaaa aataacactg
     7561 aataggattt tcagaaaagt aagcatggtt ctctttctac gcggcgttgg atttgcggcg
     7621 tatacgtatg attcgcatcc aatccagaac cttcataatc ggataggtcg gatccaattc
     7681 gaaccaacgc acggcaaaat taggccggtt agggtattta tgatgattat tttggaataa
     7741 ttcgcccaac aacataaagt cccagacgag tgagtttttc gatttatcac gattgtcaaa
     7801 attggcatac ccgtacttat gtccgcacca gttgacaatc gcaccgtgta tcggacccat
     7861 caaaaaatgc agaggtaaaa gggcgtataa ccaggtatat ccttccggga cgaaaaaata
     7921 ataaaagata aaatatccga cgccccacgc gacgcgcacg acccaggcat tggacatcgc
     7981 atcgagccaa ccccagcgcg gcgtatcgcc gtcaaattgt tgttcgggaa tagtttttcc
     8041 cgtcaacagg tcaaaataaa cttttttggt atgccacatt aggcgaaaca catccttaaa
     8101 aaaatgcggt gaatgcggat ctttttcggt atcactgaaa gcgtggtgca ttcgatgcat
     8161 cacggcgtat gcacgcggat tgagaaacga cgatccttgc gtcagaaacg tgaaaaaata
     8221 aaagaacctt tcccaaaacg cattcatatt aaacattttg tgagccgaat aacggtgtaa
     8281 aaagaaactt tgggaaaaca aggacgtgaa ccaatgtcct gcaaaaaaaa tcgcaatttc
     8341 catacgacat cttcctcgcg tttaaatagt agagttaaaa tgtttaatat ggacaatcaa
     8401 atgttaaaca aaataaaact attgtcaagt agaaaatagt gctattttat tagcatttat
     8461 tagccatatt tgagttgttg tgtaatattt tatatataat tatttgtttt atttaattta
     8521 ttaatgaata aacaatttta tatgtggctt aagagtgcca ccacggtacg tgcaggtatt
     8581 aaatgcgcgc gccatactac agtgtagcac ataaaaataa aaaaaggata aaaagattct
     8641 aacctaattt aaaagttttg cggtggattc gtaatgattg gattttgcta taaaagtact
     8701 tgtttttgca gggtcggata aatatattgc cgcgcctttg aataaaacac gtaatttatt
     8761 gtttttgttt ttgataaata tcctggagtc ttatgatcga agatgtgatt tctgaagaaa
     8821 aaaaggggag tgcccctgcc atgaagcgcc gaacggacat tcgcaatgtg gctatcatag
     8881 cgcacgttga tcacggcaaa acaaccttgg tggatcagct cttgcgtcaa agcggcacgt
     8941 ttcgtgaaaa tcaacagatc gccgaacgcg tgatggactc caacgatctc gaacgtgaac
     9001 gcggcatcac cattttagct aaaaacacgt cgattcgtta caaaggtgca aaaatcaata
     9061 tcgttgatac gcccggtcac gccgactttg gcggtgaagt ggaacgtatc atgacgatgg
     9121 tggatggcgt gttgctgtta gtggatgcgt ttgacggacc tatgcctcaa actaaatttg
     9181 tactcaaaaa agctcttgct tccaactgta caccgatcgt cgtgatcaat aagattgacc
     9241 gtcccggtgc acggccgcac gaagtgctcg acgaagtatt taatcttttt ctcgaactca
     9301 acgccaacga cgagcagttg gacttcgcca ccatctacgc gtcggctaag atggggtttg
     9361 cgcagtatga gattgatcag gccaatagcg acatggtgcc gctgttcgat acgatcctcg
     9421 aacgtatccc gagtcctccg ggcgaacggg aagcggcgtt tcaaatgctc gtagccaata
     9481 tagactattc cgattatctc gggcgaattg ctatcggacg cattcagcgc ggcgaaatca
     9541 aaacaggcca aaatgtggcc gttatgaaaa aagacggcac gcgctacaac agccgtgtgg
     9601 tgaaactttt cgcctacgaa ggcctgaaac gcacggaaac ggaaagcgcc tactccggtg
     9661 aaatcgtagc catggcgggt atggaggaaa ttcagatcgg tgaaacgatc gcggattaca
     9721 ataatccgga agcgctggaa ccgatacatg tggatgaacc tacgattgcg atgaatttta
     9781 tcgtcaataa ttcacccttc gccgggcaag acggaaaatt tgtaacgtcg cgccatttgc
     9841 gcgaacgttt gtataaagaa ttattgtcca atctcgcatt gcgcgtcgaa gataccgaaa
     9901 gtatggatac gttcaaagta tccggacgcg gtgaactgca cctttgtatt cttatcgaaa
     9961 atatgcggcg tgaaggcttt gaatttcaag tgtcgcgtcc cgaagtgatc atgaaacgcg
    10021 tagacggcca attacttgag ccgatggaat atctgatcat tgacgtaccc gaagagtata
    10081 tgggtgttgt gatcgaaaaa ttgggtaagc gtaaagccga gatgaaaaat ctgattcact
    10141 tggacaataa acaggtgaaa atcgaatttg ttattccggc gcgtggattg attggttatc
    10201 gcggtgaatt tctcaccgat acacgcggaa ccggcgtact taatcatatt tttcacgatt
    10261 acgcaccgta caaaggcgat atcggcggac gcttaaacgg tgtcttgatc tcgatggaac
    10321 aaacggaaac cgtagcctat gcattgtttg gtttgcagga acgcggcgaa ttgatcgtgg
    10381 atcctcagac accggtttac gaaggaatga tcatcggtaa acattcccgt gaaaacgatc
    10441 ttgtcgtgaa tgttggaaaa ggaaaaaaac tgaccaacat gcgtgcttcc ggttcggatg
    10501 atgcggttcg gttggaaccg gtgcgtccga tgagtttgga agattatatt gagttcatta
    10561 atgatgacga attggttgag attacgccta aaacgttccg tcttcgtaaa aaagtactca
    10621 aagaatcgga tcgtcgtcgt gaagagcgta agaaaaaaga ttaaggatca gactacagga
    10681 taaaatatgt tcaatgatct ttcggccaaa atagaaggcg ctttcaagcg tcttgccggg
    10741 cgcggcgtta tcaatgaaaa gaatatccag gaagcgttgc gcgaaattcg tatggcgctt
    10801 ttggaagccg acgtcaattt taaagtagtc aaagacttta ttgataaagc tacggccaaa
    10861 gcgctcggac aggaagtgtt gcgcagcgtt tcgccgggtg atcagctcgt caagatcgtg
    10921 tatgatgaat tggtcgagac gctcgggcac gaacacgcgc c
//
LOCUS       DAOQNQ010000084        10888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2152_length_10888_cov_6.298902, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000084 DAOQNQ010000000
VERSION     DAOQNQ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10888
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2152_length_10888_cov_6.298902"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="PK754_07010"
     CDS             <1..998
                     /locus_tag="PK754_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB09289.1"
                     /translation="VAGWILELDRGEGIPFEGNYTSWLEQKKKRLEVEEKQESKRQKA
                     LAEELEWVRMNPKGRHAKSKARISAYEKMVNEQNEKRNEEMEIFIPPGPRLGNVVIEA
                     NGVGKSYDDRLLYENMNFSLPPGGIIGVIGPNGAGKTTLFRMITGGEKPDAGSIRIGE
                     TVKLGYVDQNRPLDPNKSIWEEISGGEETLMLGTREVNSRAYVARFNFSGSDQQKKVS
                     MLSGGERNRVHLAKMLKEGANVLLLDEPTNDLDVNTLRALEEALLEFAGCAVVISHDR
                     WFLDRVATHILAFEGDSQVVWFDGNFSQYHENFKQRMGIDADRPHRIKYKPLMRG"
     gene            1339..3819
                     /locus_tag="PK754_07015"
     CDS             1339..3819
                     /locus_tag="PK754_07015"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNB09290.1"
                     /translation="MKNLTKKLILTSLMALMAFSSAFGQNGTLRAKFVKSTDGSPVTD
                     ATFTAVLASNNQSYGVRTPNAQGEMEWSLPPGNYYVKRQTGNYVATNSVTVTLLAGET
                     NSTLVTNPTSLTPLAFLRAKFVKSTDGTPVTDATFTAVLTSNNQNQGTRTPDAQGEME
                     WALPPGNYYVKLLTGYYVATNSVTVTLIAGETNSTLVTNPTSLTPSTVLRAKFVKSTD
                     GSPVTDATFTAVLASNNQNYGVRTPNAQGEMEWVLAPGNYYVKPITGNYVSANSSTVT
                     LVAGETNSTLITNPTSLTPLATLRAKFVKSTDGTPVTDATFTYVYSAGNYSGTLYPDA
                     NGIITQLLPPGDFKVKTLSGNYVGYTSGTTTVTAGEINAAFVDTPRVLTPLGVKAHFV
                     NSNNTAVISGKTVKITDGSNNTVATQTTNANGDVYIQLTPGSYTMTAFDNDANYQTQN
                     IPITIPSNAVLDKGTINMAQNKFALAFNDAGDYVNAGQESSLNTISSFTIEAWVLRKS
                     GGNSQHIISKGGGWGNNGVNIHFYYDPTPNPNPASTSLRVDFRGTNPAGWFFYNVDDQ
                     VGFDQWHHISIVWDRTTLTTPRVYVDGIYEHVGTGSPYSGYIYTDNYDINIGRNSSIN
                     SAYFKGNLDEVRWWNFARTQSEIQASMNTHLNGNESGLVLYYKLDEGSGSLINDIAGT
                     NSQGTLMGNTSWLTTTFPHISGTMTKTLAGTFYESAYTDTTGEMQKDLSTIPMEFGLE
                     SNFPNPFNPTTTLRYALKEDVRVSLKVYNLLGQEVRNLVNEHQTAGFKNVLWDGKNNQ
                     GKQVPSGVYIYRLEAGSFKMSKKMNLIK"
     gene            4221..5564
                     /locus_tag="PK754_07020"
     CDS             4221..5564
                     /locus_tag="PK754_07020"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09291.1"
                     /translation="MKSMFKSLHLVFMSLAIMAMLGCEEKTNSNQEIKSGGENFNLFT
                     GIDGTEIENLRSEYSKTFQLSNGKVVTRFSAQKVHELVNGQWIDIDYNKKPEKSAGII
                     EGSITGTLKYTRISNNITTTEYAPIFDNLGSYPGYLPDDQSVTYNNENSRLVVTFNGI
                     NNIPSGYRVRSSELALSNSIPTSGSVSRSEIYGDIDIKLFTSNSLYGTQVHYDACSVG
                     TTLGTKKTGFFPVILDNSSLNNAVINAIPNNRVSFSLVSVGALCSITYTHLDIYLELI
                     PRYSITTVVNPSGSGTISLSPAPGADGKYEQGTLVTATASPSSGWQFYNWSDANNSQS
                     NQISFTVNSNASLNANFIKQLQINGPVELPTNGSATYTVTTIPDATTYKWYKLVKQTT
                     YPYGTTYSYQSTGTSYVGVMGTGAIPLVLIMKVEAYDASNALRGSGIIAVEASEN"
     gene            5719..7233
                     /locus_tag="PK754_07025"
     CDS             5719..7233
                     /locus_tag="PK754_07025"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNB09292.1"
                     /translation="MTSQSSQHHKETREVARGAFINLIGNFGKLSHFAFDLIATRVLG
                     QTLFGYFSTTWLLMHLSFIVCYFGAHRLVIDFVVRYKDTDEDEYYKSILGYIILSLGL
                     SAFLVLNVYLFADHLAAYMGKPPVADYMKIMVWCAPFYCLTTILMTATRGLKIMKLWV
                     FIRNGFEPLVDLILLLVVFFVFGSIAAPFWAKMIAFTLGSGLSIYYFRKHFSMRRIFS
                     HLPDGSTWKRIFSFGFPVMFADFISVVILKVEIIPLSILVPSAQVAVFQVILNTGNTM
                     RNIPQAIDPIMMPVVVAMRQRNDMRALEGIFATLIRSSLFLACGFFVLTTIYGDFLLR
                     IFADEFAYAAGALSIVCFGMMIHTVTSNIEPVLVMSGYPYLNLFNNVFLVAVSMTLDF
                     ILIPTYGIFGAAIGNLTGCLLTAALQIAEVHLILKIRPFRRDLLHIVWIGLGCYIIFK
                     GLDFLFPAWTDLLIYDIICVCIFTAFYLYVGWKLFLHDEERILFSSMIHKPQSE"
     gene            7263..8162
                     /locus_tag="PK754_07030"
     CDS             7263..8162
                     /locus_tag="PK754_07030"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNB09293.1"
                     /translation="MKITAFFLFVSFSASQLFAQAAESKTTVAVLPPDITKIVTSAAT
                     GSSEVNVIADALTQKIVSTLVGLKRIAVVERAAMDKILSEQDFQMSDLAAENTPVRLG
                     ELMGADFLVILHLYQAQTTLVTQTEEEKQTWGDRGTQYSGLLEYGIRLVQVSTGEVTT
                     TKNLKGDTGFLAYTTTTEALSNALNMSSDKLKDWLKSSFPVRGKIFEILKEKKGEAVS
                     VAITCGSASGVRKNDAFKVVEYTEVELDGKKVKRSKDIGKIKVSKTESDGIFSRCEVI
                     NGGKTITSKMKSGSQLDVILVKD"
     gene            8197..>10888
                     /locus_tag="PK754_07035"
     CDS             8197..>10888
                     /locus_tag="PK754_07035"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNB09294.1"
                     /translation="MQRRTFVLLFLSLSLTSLFAQTDETIPMDPAVRSGVLSNGLRYF
                     IKTNARPEKRVELRLAVNTGSIMEDDDQLGLAHFVEHMAFNGTTHFQKNDLISYLQSA
                     GVKFGAHINAYTSFDETVYMLTLPSDKQDIIHNGFLILEDWAHGITFDSLEIEKERGV
                     VIEEWRTGIGAQQRMSDQYWPKLFYGSRYAERMPIGKKEILEKFSHASIKRFYKDWYR
                     PELMSVIAVGDIQPDSIEYLIRKHFSNIATSDAKKVRPRNDFPVPPHKETWTAVARDK
                     EAPNTMAQIFYKHPSQTVRSVQDYRTSLLRMLYTGMLNKRMEELTQQANPPFVFGGAF
                     YGNFVRATDVYAVYAGTGETALERGLLAVLIENRRAQQHGFSRDEMEDYKKEILSNYE
                     SSYNNRDKVLSSSLADEFVEYALTAEAAPGMAWEYETVKRLLPTITVDEIHALAAQWI
                     TTENRIGVVTAPQNETVKLPDEARLLALIDSVATIQTVPYVYKTVQVPLLATLPHPGK
                     IVKTKKLKEIAAEEWTLSNGMKVILKPTTYMTDEIKFSAYSFGGWSLGAPSQDMSAIY
                     GMAPLGEGGIAEFSKTDLEKILSGKNVSVSTGIRMLSETISGQSLKKDMETMFQLIYL
                     NFTAPRLDTTVFYALLSRQMAYIKNLDNDPRFVFSNELNRVLSRDHPRDPSVLRRERF
                     SEINPQTALAIYRDRFAEAGDFVMTIVGSFEIETMRPFVERYLAGLPTTGRKETFKDL
                     GIRRPGRNMDTTIYRGVDPRAQVNMQFHGALKKYDDLFLLRATAMLLTNKLIENLRED
                     KGGVYGTSANAYANKYPVSNYAVIIGFSCAPERLDSLIGAVHEEIRKLAENGPAESDV
                     NKIRETLRRDLERNQKENAYWLRWIESAYYENASIKNITPE"
BASE COUNT         3156 a         2464 c         2302 g         2966 t
ORIGIN      
        1 atgtcgccgg ttggatactc gaactcgatc gcggcgaagg tattcctttc gaaggcaatt
       61 atacctcgtg gcttgaacag aaaaagaaac gccttgaagt cgaagaaaag caggaatcca
      121 aacgccaaaa agctttggct gaagagttgg agtgggtgcg aatgaatccc aaaggtcgcc
      181 acgccaaaag taaagcacgt atttcggcgt atgaaaaaat ggtcaatgaa cagaatgaaa
      241 agcgtaacga agaaatggaa attttcattc cacccggacc gcgcctcggc aatgtcgtca
      301 tcgaagcaaa cggcgttggc aaatcctacg atgatcgttt gctttatgaa aacatgaact
      361 tttctctgcc acccggcggt atcatcggcg tgatcggacc caacggtgct ggtaaaacca
      421 cattatttcg tatgattacc ggcggggaaa aacccgacgc cggctcgatt cgtatcggcg
      481 aaaccgtcaa actgggatat gtagatcaaa atcgtccact ggatcccaat aaatcgattt
      541 gggaagaaat cagcggcggc gaagaaacgc tgatgctggg tacccgtgaa gtcaattcgc
      601 gtgcctatgt agcgcgtttt aactttagcg gttcggacca acagaaaaaa gtcagcatgt
      661 tatccggcgg cgagcgcaac cgcgttcacc ttgccaagat gctcaaagaa ggcgccaatg
      721 tactgctgct tgacgaacct acaaacgatc tggatgtcaa tacactgcgc gcactggaag
      781 aagcgcttct ggaatttgcc ggatgtgccg tggtgatttc ccacgaccgt tggtttctag
      841 atcgtgtagc aacccatatc ttagcattcg aaggcgacag ccaggtagtt tggtttgacg
      901 gaaatttttc gcaatatcac gaaaacttca aacagcgcat gggcattgac gccgatcgcc
      961 cgcaccgtat caagtataag ccgctgatgc gaggataatg tcagaattta agattgcata
     1021 gaatatttta gccccgtttg aaattcaaat ggggcttttt ttgcttgcct tggcgtttag
     1081 attcatttat actgaagtgt tagaaggaaa atcgttttta ggggtataaa gagaacgtat
     1141 tatatcgtat ttcgcaatta atcccatttc ccattcattt ttcaacacaa acacagcaca
     1201 tgtgtgtggt cgtgtattta ttacactcat ataatcgtca agatttctga aatgttttac
     1261 gttttttgta ctacctaatc taggtagcac aaaactatcg ttttaattaa ccatgtttca
     1321 caaaatgagg agtagggcat gaaaaacctt acaaaaaaac tgattctcac atcactgatg
     1381 gctctcatgg ctttcagttc tgccttcggg caaaacggta ctttacgggc aaagttcgtg
     1441 aaaagtactg atggttcgcc ggtaactgat gctacgttta cggccgtatt ggcttcaaat
     1501 aatcaaagtt acggtgtacg gacgccaaat gctcagggtg aaatggagtg gtcattaccg
     1561 cctggaaact attacgtaaa acggcaaaca ggaaactatg ttgccaccaa ctcagttacg
     1621 gttaccttat tagcagggga gacaaattca acactcgtga ccaatcctac atctctaacc
     1681 ccgctcgcct ttttacgcgc taaattcgtg aaaagcacag atggtacgcc agtaaccgat
     1741 gcaacattta ccgcagtatt aacctccaac aatcaaaacc aaggtacgcg cacaccagat
     1801 gctcaaggtg aaatggagtg ggcattacct ccaggaaatt attatgtaaa gctcctaaca
     1861 ggatactacg ttgctactaa ttctgttaca gttactttaa ttgccggaga aactaattct
     1921 acgctcgtaa ccaaccccac ttctttgact ccgagtaccg tcttacgcgc caaattcgta
     1981 aaaagcaccg atggatcgcc tgtaacagat gctacattta cggccgtatt ggcttcaaat
     2041 aatcagaact atggtgtgcg cacaccaaac gctcaaggtg aaatggagtg ggtattagct
     2101 cccggaaatt attacgtaaa accgattaca ggaaattacg tcagcgccaa ctcaagtacg
     2161 gttactttag tcgccggcga aactaattct acactcataa ccaaccccac atctttgact
     2221 ccacttgcca cactacgtgc taaatttgtg aaaagcactg atggtacgcc ggtaaccgac
     2281 gccactttta cttatgttta ttccgccgga aactattctg gaacacttta tcctgacgcc
     2341 aatggtataa taacacaatt actgccacca ggtgatttta aggttaaaac cttatctgga
     2401 aattatgtgg gctatacatc cggaacaact acagtaaccg ccggcgaaat aaatgctgcc
     2461 tttgttgata caccgcgtgt tcttacccct ctcggtgtca aagcgcattt tgtcaattcc
     2521 aacaataccg cagtgattag cggaaaaaca gtcaaaataa cagacggttc taataataca
     2581 gtagccacac aaaccaccaa tgccaatggc gacgtgtata tccaactaac acccggttca
     2641 tatacgatga cggcttttga caatgatgca aattatcaaa cacaaaatat tccgattacg
     2701 attccgagta atgccgtttt ggataagggc accattaata tggcacaaaa taagtttgct
     2761 ttagcattta atgatgcggg cgattatgtc aacgccggtc aagaatcatc cttaaacact
     2821 atatcatcgt ttaccatcga agcatgggta ctgagaaaat cgggcggcaa tagtcaacat
     2881 attatcagta aaggtggcgg atggggaaac aacggcgtca acattcattt ttactatgac
     2941 ccaacgccta atcctaatcc cgcttcaacc tcactacgtg ttgatttcag gggtaccaat
     3001 cctgcgggat ggtttttcta caacgtcgac gatcaagttg gatttgatca atggcaccac
     3061 atttccatcg tttgggacag aaccaccctc acaactccaa gagtatatgt tgatggaatt
     3121 tatgaacatg taggcacagg ttccccttat tccggttaca tatacaccga taactacgac
     3181 atcaatatcg gacgcaatag cagcatcaac agcgcatatt tcaaaggcaa cctcgatgaa
     3241 gttcgctggt ggaattttgc tcgcactcaa agtgaaatac aagcttcaat gaacacgcat
     3301 ttaaatggaa atgaatccgg gcttgttctt tattacaaac tcgacgaggg ttccggatct
     3361 ctaatcaatg acattgccgg aactaatagc caaggaactt tgatgggaaa cacatcctgg
     3421 ttaaccacaa ctttccctca tatatctggc accatgacaa aaactctggc cggcacattt
     3481 tatgaatcag cctacacgga cactacgggt gaaatgcaaa aagatctgag tacaattcct
     3541 atggaatttg gcttagaatc caacttcccc aatcctttta acccgacgac gacgctgcgc
     3601 tatgcactca aagaagatgt gcgcgtcagt ctgaaggtat ataatcttct tggtcaggaa
     3661 gtgcgtaacc tcgtcaatga gcatcagaca gccgggttca aaaatgttct ttgggatggc
     3721 aaaaacaatc aaggcaaaca agtaccgagc ggtgtctata tttaccgact tgaagccggt
     3781 agctttaaaa tgtccaagaa aatgaatctt attaagtagt cgtgttctat attataccag
     3841 ccccatccgc ttatgcagat ggggcttttt tttgccttgg cgtttagact cacttatgct
     3901 aaagtgttag caggaaaatc gtttttaggg gtataaaggg aatgtattat atcgtatttc
     3961 gcatttaatc ccatttttcc cattcatttt tcaaagcaaa tacagcaatg tgtgtggtcg
     4021 tgtatttatt acactcatat agtcgtcaag atttcttgaa atgtttttac gtttttttgt
     4081 actactaaat ataggtagca caaaactatc gttttaatta actatgtttc agaaaataag
     4141 gagcctgttc caacatcaat tattagttaa taatgtttaa ttctctaaca aatctcaatt
     4201 tattaaaaaa gggaaagcgc atgaaatcca tgttcaaaag tttgcatttg gtttttatga
     4261 gtttggctat aatggccatg cttggttgtg aagaaaaaac caacagcaac caagaaataa
     4321 aaagtggtgg agaaaatttc aatcttttta caggtattga tggcacagaa atagagaatc
     4381 ttagatcaga atactcaaaa acgtttcaat taagtaatgg gaaagtagta acacgtttca
     4441 gcgcacaaaa agtgcatgaa ttagtaaatg gccagtggat agacattgac tacaataaaa
     4501 aaccggaaaa aagcgcagga atcattgaag gctcaatcac tggaacttta aaatatacac
     4561 gaatatccaa taatatcacc accacagaat acgcacctat ttttgataac ttgggtagtt
     4621 atcccgggta tctgcccgac gatcaatccg ttacttataa caacgaaaat tcaagattag
     4681 ttgttacttt taacggcatc aataatattc cttcaggcta cagagtgagg tctagtgaat
     4741 tagcacttag caattcgatc ccgacttccg gctcagttag cagatctgaa atttatggtg
     4801 acattgacat taaattattt acatcaaact ctttgtatgg cactcaagtt cactacgacg
     4861 cttgctcagt tgggacaact ctaggtacca aaaaaaccgg ctttttccct gtaatattag
     4921 ataattcatc actcaataac gctgtaatta atgccattcc aaataataga gttagtttta
     4981 gtttggttag cgtcggagca ctctgtagta ttacatatac tcatttggat atttatttag
     5041 aattgattcc gcgttatagt attacaacag ttgtaaatcc aagcggtagc ggcacgattt
     5101 ccttatcgcc agcaccagga gccgatggta aatatgaaca aggaactctt gtaacggcta
     5161 cagccagccc gtcatccggt tggcaattct ataactggtc agacgcaaac aactctcaat
     5221 ccaatcaaat ttcattcaca gttaattcaa acgcatcact taatgcaaac tttataaaac
     5281 aactgcaaat caacggaccc gttgaactcc caacgaatgg tagtgcgacg tatactgtca
     5341 caaccatccc agatgcaacg acttacaaat ggtataaatt ggttaaacaa acaacatatc
     5401 cttatggaac tacatacagc tatcaaagta caggaaccag ttacgtgggc gtaatgggta
     5461 ccggcgctat tcctctcgta ttgattatga aagtagaagc ttatgatgca agcaatgctt
     5521 tgcgaggtag cgggatcata gctgtcgaag cctcagaaaa ctagccatct tggaatcaca
     5581 ttaattgaaa gattatgagc cccatccgct tatgcagatg gggctttttt ttgtctgttt
     5641 tttttgttat gaatgcagca aatgtatttc aggacgcctt gatacggcgt tctttcatct
     5701 aaagagtatc attcgttttt gacatcccaa tcgtcgcaac accacaaaga aacccgcgaa
     5761 gtcgcccgtg gggcttttat caacctcatc ggcaatttcg gcaaactatc tcattttgcg
     5821 tttgatctga tcgctacgcg tgtgctcgga caaactcttt tcggttactt cagcacgaca
     5881 tggcttttga tgcacttatc atttatcgtg tgttatttcg gcgcacaccg gctggtgatt
     5941 gattttgtcg tacgctacaa agataccgac gaagacgaat attataaaag catccttggc
     6001 tatatcatcc tgagtttagg actcagcgct ttcttagtac tcaatgtcta tcttttcgcc
     6061 gatcatctag ccgcttatat gggcaaaccg cccgtcgccg actatatgaa aatcatggta
     6121 tggtgtgcac cgttttactg ccttaccacc atcctcatga ccgccacacg cggcctcaaa
     6181 atcatgaaac tttgggtttt tattcgcaat ggatttgaac cgttggtgga tctgattctg
     6241 cttctcgtcg tgttttttgt tttcggttcg atcgccgcac cgttctgggc caaaatgatc
     6301 gcatttacgc tcggtagcgg gttatcgata tattatttta gaaaacattt ttcgatgcgg
     6361 cggatttttt cacatcttcc ggatggttcg acttggaaac gcattttttc tttcggcttc
     6421 ccggtgatgt ttgccgattt tatttcagta gtcatcctta aagtcgaaat cataccgctc
     6481 agtattctcg tgccgagcgc acaagtcgcc gtttttcagg tcatcctcaa taccggcaac
     6541 accatgcgta atataccgca agcgattgac cctatcatga tgccggtcgt ggtcgccatg
     6601 cgccagcgca atgatatgcg cgccctggag ggtatttttg ctacgctgat tcgctcaagt
     6661 ctttttttgg cctgtggctt ttttgttctt acgacgattt acggtgattt tttactgcgt
     6721 atatttgctg acgaatttgc ttatgccgcc ggcgcattat ctatcgtatg cttcggtatg
     6781 atgattcata ccgttaccag caacattgaa cccgtccttg tcatgtcggg atatccttat
     6841 ttgaatttat ttaacaatgt atttcttgtt gctgtgagca tgacccttga ttttatactt
     6901 atacccacgt acgggatatt tggtgcggcc atcggcaatc tgaccggatg tttattaact
     6961 gcggctttac aaatcgccga ggtacatctc atactcaaaa tacgtccttt tcgtcgggat
     7021 cttcttcata tcgtatggat tggtcttgga tgttatatta tttttaaagg cttggatttt
     7081 ctttttccgg cgtggaccga tcttttgatt tacgatataa tatgtgtttg tatttttacg
     7141 gcgttttatc tttatgtcgg atggaaactg tttttacatg atgaagaacg tattttattt
     7201 tcatccatga tacacaaacc ccaatccgaa tagtcaccgt attttataaa aggaagtaac
     7261 cgatgaaaat caccgctttt tttttatttg tttcattctc agcttcgcaa ctattcgcgc
     7321 aagctgcgga atctaaaact actgtggccg tactgccgcc ggatattacc aaaatcgtaa
     7381 ctagcgccgc gaccggctca tcggaagtca acgtcatcgc cgatgcgctg actcaaaaaa
     7441 tcgtctcgac gctcgttggg ttaaaacgaa tcgccgtcgt agaacgcgcg gcaatggata
     7501 aaattcttag cgaacaggat ttccaaatgt cggatctcgc cgctgaaaat acaccggtac
     7561 gattggggga actgatgggc gcggattttc tggttatttt gcatctctat caggcacaga
     7621 cgaccctcgt cactcagacc gaagaagaaa aacaaacctg gggtgatcgt ggtacgcagt
     7681 atagcggcct tctagaatat ggcatccgcc ttgtgcaagt atctacgggc gaagtaacta
     7741 ccaccaaaaa cctaaaaggc gacacggggt ttctcgctta tacgaccacg accgaagcac
     7801 tatcgaacgc actgaatatg agcagcgaca agctgaaaga ttggctcaaa tcatcgtttc
     7861 cagtgcgagg gaaaattttt gaaattctaa aagagaaaaa aggcgaggcc gtgtccgtcg
     7921 cgatcacttg cggcagcgca tccggtgtac gaaaaaacga tgcctttaaa gtcgtcgaat
     7981 atacagaagt cgaattggat ggaaaaaaag taaagcgctc caaggatatc ggaaaaatca
     8041 aagtcagcaa aaccgaatcc gacggtattt tttctagatg cgaagtcatc aacggcggta
     8101 aaaccataac atcaaaaatg aaatcaggct ctcaattaga tgtgatactg gtcaaagatt
     8161 gatcttatag atatgtaaaa aataaaggac atgatcatgc aacgaagaac gtttgttctg
     8221 ctttttttat cgcttagtct tacttcgcta ttcgctcaaa ccgatgaaac gatacctatg
     8281 gatcctgcgg tacggagcgg cgtattgtcc aacgggttaa gatatttcat caaaaccaat
     8341 gcgcgcccag aaaaacgcgt ggaattgcgt ttggcggtca ataccggctc catcatggaa
     8401 gatgacgatc aacttggtct ggcgcatttt gtagaacaca tggctttcaa cggtacgaca
     8461 cattttcaaa aaaatgattt gatcagctat ttgcaatcgg ccggcgtcaa atttggtgca
     8521 catatcaacg cgtatacgtc gttcgatgaa accgtgtaca tgcttacact acccagcgac
     8581 aaacaggata ttattcacaa cggattcctc attctggaag actgggcgca cggcatcaca
     8641 tttgacagcc tcgaaatcga aaaggagcgt ggcgttgtga tcgaagagtg gcgtaccggt
     8701 atcggcgctc agcaacgcat gagcgatcaa tattggccca aattatttta cggttcgcgt
     8761 tatgccgaac gtatgccgat cggaaaaaaa gaaattctcg aaaaattttc ccacgcttcg
     8821 atcaaacgtt tttacaaaga ttggtaccgc cccgaactga tgtccgtcat cgcggtcggc
     8881 gacatccaac cggactccat cgaatatctg attcgcaagc atttttcaaa tattgctacg
     8941 tcggatgcca aaaaagtgcg cccgcgtaat gattttcccg tacctccgca taaagaaaca
     9001 tggactgccg ttgcgcgtga caaagaagcg cccaacacaa tggcccagat tttttacaaa
     9061 cacccatccc aaaccgtacg ttcagttcag gattatcgta cgtccctatt gcgtatgctg
     9121 tacaccggga tgctcaataa acgcatggaa gaactgactc agcaagccaa tcctcctttt
     9181 gtttttggcg gagcttttta tggtaatttt gtgcgtgcca cggatgtgta tgccgtgtat
     9241 gcgggtacgg gcgaaacggc tctggaacga ggactcctcg ccgtactcat cgaaaatcgt
     9301 cgtgcacaac agcacggttt ttctcgcgat gaaatggaag attataaaaa agaaatcctt
     9361 tcgaactatg aatcttcata caataatcgc gacaaagtac tttcttcatc ccttgccgat
     9421 gaattcgtag aatatgcgct taccgccgag gccgcacccg gcatggcatg ggaatacgag
     9481 accgttaaac gtcttcttcc cacgatcacc gttgatgaga ttcatgcttt ggccgcccaa
     9541 tggattacca cggaaaatcg tatcggcgtc gtcaccgcac cccaaaatga gaccgtaaaa
     9601 cttcccgacg aagcgcgttt gcttgcgcta atagactctg tagcaacgat ccaaacagta
     9661 ccgtacgtat ataaaaccgt acaggtaccg cttttagcaa cgctgcccca tcccggcaaa
     9721 atcgtcaaaa caaaaaaact caaagaaatt gccgctgaag aatggacgtt atccaatgga
     9781 atgaaagtga ttttgaaacc gaccacctac atgaccgatg aaatcaaatt cagcgcatac
     9841 agctttggcg gatggtcgct tggtgcaccc tctcaggata tgtctgctat ttacggcatg
     9901 gcaccactgg gtgaaggagg catcgctgag ttttctaaaa ccgatttgga aaaaattcta
     9961 tccggcaaaa acgtatccgt ttcgacgggc attcgcatgt tgagtgaaac catatccggg
    10021 cagtcgctta aaaaggatat ggaaaccatg ttccaactga tctatctcaa ctttactgca
    10081 ccgcgattgg acactacggt tttttacgcc ttgctcagcc ggcaaatggc ctacatcaaa
    10141 aacctggaca acgatccccg ctttgtgttt agcaacgaac tcaatcgtgt tctcagccga
    10201 gatcatcccc gtgatccctc agttttgcgg cgtgaacgtt tttcagaaat caatccgcaa
    10261 acggcattgg cgatataccg tgatcgtttt gccgaagccg gcgatttcgt aatgacgatc
    10321 gtcggcagct ttgagatcga gacgatgcga ccttttgtcg aacgttattt ggccggttta
    10381 cctacgaccg gacgtaaaga gacattcaaa gatctcggaa ttcgcaggcc gggtcggaat
    10441 atggatacca cgatttatcg cggtgtagat ccgcgtgctc aggtcaatat gcagttccac
    10501 ggtgctttga aaaaatacga cgatcttttt ttattgcgcg ctacggcaat gcttttgacc
    10561 aataaactca tcgaaaacct ccgcgaagac aaaggcggag tgtatggcac aagcgccaat
    10621 gcgtacgcca ataaatatcc ggtatctaat tacgccgtga tcatcggttt ctcgtgcgca
    10681 ccggagcgat tagattccct gatcggtgca gtgcacgagg agattcgtaa attagcagaa
    10741 aatggtccgg ccgaatccga tgtgaataaa atccgtgaaa cattgcgccg tgatctggag
    10801 cgaaatcaaa aagagaacgc ttactggtta cgatggatcg aaagcgctta ttatgaaaac
    10861 gcttccatca aaaacattac tcccgaag
//
LOCUS       DAOQNQ010000085        10768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2191_length_10768_cov_5.446560, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000085 DAOQNQ010000000
VERSION     DAOQNQ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10768
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2191_length_10768_cov_5.446560"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PK754_07040"
     CDS             complement(<1..463)
                     /locus_tag="PK754_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNB09295.1"
                     /translation="MKRIAVIPGDGIGTDVINEALKVIEAVQTRKPMDIQLTTMDYGA
                     EKYLKTKVTMPEEQYDDFRNNYHAILMGAFGDPRVPGNEHAKDILLGTRFKLDLYVNF
                     RPVKLLDERFCPLKNKTVHDVNFTVFRENTEGLYVSVGGIFKKNTPDEIAIQ"
     gene            639..1340
                     /locus_tag="PK754_07045"
     CDS             639..1340
                     /locus_tag="PK754_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNB09296.1"
                     /translation="MSISLQEYFHAIAEATYGKIGDDFFKSLVRNLSSVLKMEFAMVT
                     ECANSEKSRLRTIAHWIGMDFGKNFEYDITHTPCHMVIHGGLQCYTEDLPAQFPLEKE
                     LSEIQATSYIGIPLKKSNGEIVGHLALLDTRPILDAEIKASILKIFASRAEAELERLQ
                     DKRERELLIYELQESLELVQTLRGMLPICASCKKIRDDKGYWNRIEEYVERHSGAQFT
                     HGICPECSVKLYPDL"
     gene            complement(1359..2282)
                     /locus_tag="PK754_07050"
     CDS             complement(1359..2282)
                     /locus_tag="PK754_07050"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNB09297.1"
                     /translation="MKRGKFFLFTGLSILLLFICACQKKKEPIITDAAYVQEINAWHA
                     QREANLKKEKGWLNLVGLHWLKEGDNTFGSDSSNQLIFPPQAPPFAGTITLQKGGITL
                     TVKKDVVITADGKTISTSVLKDDSDSLMTRLAMGSFRFYVIKRNGEYAIRLKDLESPL
                     LKSFQGIDRYTVDKNWQTHATIKKYDPMKIILIPTILGTVDSMKCPGLLTFTIDDQTY
                     TLEPVLEDGSDDLFIIFGDRTNGSETYGGGRFLYTKMPAQGDSVIIDFNKAYNPPCVF
                     TDYATCPLPPLQNKLPIPIKAGEKMWGHKKH"
     gene            complement(2269..3405)
                     /locus_tag="PK754_07055"
     CDS             complement(2269..3405)
                     /locus_tag="PK754_07055"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNB09298.1"
                     /translation="MIKPGSTLGMLGGGQLGRMFTMAAHAMGYRVIVLDPDPQSPAGH
                     IADRHLTADYHEAEALHILRTECSAITTEFENVPADTLEILSHHCFIRPSAKAVSVTQ
                     DRLHEKSFLRIHQIPTNKFAAIRTESDIDEAVHFVGLPAILKRNRYGYDGKGQYRVRT
                     VQDVRNAFRAMHDAPCILEEMLNFEKEVSVVLARAVDGSITEYPTGENIHENGILDMT
                     IVPARIAGTMDSQARTIARQVAEKLDYHGVLAVEMFVMPDGRVLVNEMAPRPHNSAHY
                     TLDACITDQFEQQVRVLCGLPAGDPSLLSPVVMVNLLGDLWGNQQPHWEKLFTQTHVK
                     LHLYGKSEARPGRKMGHFNILDRNIENAIALGRQLKKDVAYETR"
     gene            complement(3450..3941)
                     /gene="purE"
                     /locus_tag="PK754_07060"
     CDS             complement(3450..3941)
                     /gene="purE"
                     /locus_tag="PK754_07060"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNB09299.1"
                     /translation="MAQKILIGVVMGSDSDWEVMKYAVEQLKTFKIPYEARVVSAHRT
                     PELLFSYAASAQERGLRCIIAGAGGAAHLPGMLASKTIVPVLGVPVPSRYLKGLDSLL
                     SIVQMPRGIPVATFAIGESGAANAALQAIAILATTDDTLAKKLSAFRESQTRKVLQLK
                     LPK"
     gene            4119..4583
                     /locus_tag="PK754_07065"
     CDS             4119..4583
                     /locus_tag="PK754_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNB09300.1"
                     /translation="MFKRVIETSDDSILTILRVVSGSVILMHGLQKLLGWFGGHGVTA
                     TMQAFEAWFGMPYIVTLLVILSDSLGAICLIAGAVTRFMAASILSVMIGAIILVHGRW
                     GFYMNWYSEKRGEGFEFHLLVIAIMIVLIMRGGGLWSVDRWLVKKFKATDNI"
     gene            complement(4656..5426)
                     /locus_tag="PK754_07070"
     CDS             complement(4656..5426)
                     /locus_tag="PK754_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09301.1"
                     /translation="MKKIILLFICAVSAAFAQESNPATPYQHRQLVNVPTAGMLNKGN
                     YEIGMRLFANGGVLSNIGVGITDRFMIGISYGGEKIIGSGKVNFNPLPGVDVRYRAVD
                     ESTIGPAVTLGFNNQGFGPYYKSVDTVKVNRYTQKSRGLFITASKNYAFAGNLGIHGG
                     ISWSVTEKKDKDNQPAFFVGVDKSINDELTAVWEYDLALNDNKDIIGHGRGYMNVGAK
                     LNFGQKVMVEFIMTDILNNSKDIGKFSRELRLSLIHAF"
     gene            complement(5495..6670)
                     /locus_tag="PK754_07075"
     CDS             complement(5495..6670)
                     /locus_tag="PK754_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09302.1"
                     /translation="MATSTAYAGGSSYSATTYGLYEYKPNPRSLGMGGAGLAIPSYYT
                     LNMQNPATLLGIPMTRFELNMFAEWNQASNGASKNFSNRATINHVAFAFPFGKTLVAA
                     INLSRVTNVNYEFSTPNLIFEGEKYSETLIGTGGLQQLAFTGAGKLNPKWTIGASLQY
                     LFGTIKREWDINYSSDELVDTRDVRHQNMNGGRLVVGGLYQHNEQWNFGGFLSMAGKI
                     SSDIRVVGTYRDTTVSYEQNLNYPTEIGFGASYRWTKRYLLATDIVYGTWGSVKEFSP
                     TQKNRNTVRWSSGIERQPSNAFSAPWWDKNYYRFGLMINNLYATNTTGNHASEIMFTL
                     GIGMPFNRERSSFDLSLEAGQRGQIADNKVRDRVYRLTLSFTGGERWFLNRQKRKRD"
     gene            complement(6725..7978)
                     /locus_tag="PK754_07080"
     CDS             complement(6725..7978)
                     /locus_tag="PK754_07080"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HNB09303.1"
                     /translation="MEVIDRGTSTGTFKTDTLYADDIERIASFVDSISTYNSSRLYLG
                     SVDNYDFRILMRFAFPSDYITDPSGELINLDSLIIVKTRLQLVASDAFGSAGSINAHI
                     HTVQTPWSELDLLWHKFQQGTDYSPNSIGTINFSKLNDKDSLEITLNKDSVVQWVKAR
                     NDTSTINPKTNNGIIIDFDGGAPFTQQFYSGNNTIITINGTIIDFDLVPRIEVEYVYL
                     RDKNGDGDYEDPDDKNYRSTFYVSPVFISGVYNGGYHGYVYRDRNPQALNSITVGGGV
                     PYRSLIRFNTDKLPSDLTINSAVLVLKTEASGNYFRNSSDSINIQAMASTVDTSLWKP
                     GYIEQTTTNSRTDNIRDDTLKLNISSFVQDWATQPNHNNGLVLMNYNIFNGNFNPLYR
                     VRFYNNPSDRDNSPKIIIYYTQSPQ"
     gene            complement(8104..9087)
                     /gene="amrS"
                     /locus_tag="PK754_07085"
     CDS             complement(8104..9087)
                     /gene="amrS"
                     /locus_tag="PK754_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNB09304.1"
                     /translation="MIEAKFYRKEADHIVCELCPRMCKIQEGAEGDCWGYKNIDGKLY
                     SFNYGKIVTAVYDPMEKKPFFHYYPNSTILSLAPNGCNMRCKFCQNHDVALNKKETKD
                     ISLQTILDYSGKSHSIGVAYTFTEPLIWYEFLIDSMRKLKGIGQKNILITNGYLNQKP
                     IEDIIPYIDAMNISVKSMNAETHERLTESKLEIVLNSIKSAFGKTHLELTYLLIPGES
                     DSTEDVGNFVDFVAGINKDIPVHFKRFFPHHKMTSQPMTPNETMVRTYKLATQKLSYV
                     YLTDTDIDGVSDTFCPNCKRLIIKRRDYQSNLGGMRGSNCKFCGHRIFIKF"
     gene            9318..9899
                     /locus_tag="PK754_07090"
     CDS             9318..9899
                     /locus_tag="PK754_07090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB09305.1"
                     /translation="MSYQFKHLTPQTWNDFAALFGERGACGGCWCMAWRRSNAEFQKN
                     KGEGNRKAMQRLVKKELPIGLIMYKDVIPVGWCAFAPREEYIRLAASRIFKPVDEKPV
                     WSISCFFIHKDYRKQGLSEKLIRQVIVSAKKMGVQILEAYPSDPYDAQVPAPFVWTGL
                     TSAFVKAGFKEVARRSKTRPIMRYVVNTTDLNK"
     gene            9919..10614
                     /locus_tag="PK754_07095"
     CDS             9919..10614
                     /locus_tag="PK754_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09306.1"
                     /translation="MIFLLFFIFLAVQSAATQDTLPEFPVHFVVIDGSEEVAAVATLE
                     QMHREIEILNTYFVTEDKRTLVRFRFKSATFFDEIENTACSFVDSANEGMYIGRWANL
                     YKECADTKLRDPRAINFVIFDSYTDAQGWRSRRSRGATYAGVPVVFIDYQALGHKGSL
                     EEHEMGHAFGLGHICDSTLTEDGGQNIMHHNGECPDQKKYRAKYYTGFNAEQEAIIRR
                     AILRHRKKLGLDK"
BASE COUNT         2959 a         2220 c         2415 g         3174 t
ORIGIN      
        1 cttgaatggc aatttcatcg ggagtatttt tcttgaaaat accgccgaca ctgacataga
       61 gaccttccgt gttttctcgg aaaaccgtaa aattgacatc gtgaacggtt ttatttttaa
      121 gggggcaaaa ccgctcatcc aacaatttga caggacggaa attgacatag agatccagtt
      181 taaaacgcgt accgagaaga atatcttttg cgtgctcgtt accgggaaca cgcgggtcgc
      241 cgaacgcgcc catgagaatg gcatggtagt tattccggaa atcatcgtat tgctcttccg
      301 gcatcgttac cttggttttc agatactttt cggcgccata atccatcgtg gtcaactgga
      361 tatccatcgg cttacgcgtt tggaccgctt caatgacttt tagtgcttca tttattacat
      421 cagttcctat cccgtctccg ggaattacgg ctatgcgctt cattttgtta atccaaatat
      481 tgatattttt ttagcgcgca aaagtaagca atacacccaa aaatagcaag caaaggccta
      541 tatctaattc taccgataga gttgcttgcc aattgctcaa tgggaagtta tttttgaaag
      601 agagcaatca acaaccaagc aaatatagga attgaatcat gtctatatcg ctgcaggaat
      661 attttcatgc catcgctgaa gctacttacg gaaagatcgg tgatgatttt tttaaatcct
      721 tggtccgcaa cctcagttcc gttttgaaaa tggagtttgc aatggttaca gaatgtgcaa
      781 actcagaaaa aagcagactg cgtaccattg cacattggat cggaatggat ttcggaaaaa
      841 attttgaata tgatatcacc catacacctt gtcacatggt cattcatggc ggattgcagt
      901 gttatacaga ggatttgccg gctcaatttc ctctcgaaaa agagctttct gaaatacaag
      961 cgacgagtta tatcggcatt cctttgaaaa aatcaaacgg agaaatagtc ggccatctgg
     1021 cgcttttgga tacacgccct attttagatg cggaaattaa agccagtatt ttgaaaattt
     1081 ttgcatcccg tgccgaagcc gaattagaac gcttacagga taaacgtgaa agagagctat
     1141 tgatttacga attgcaggaa agcctcgaac ttgttcaaac tcttcgaggc atgttaccga
     1201 tttgtgcgtc gtgcaagaaa attcgtgatg ataaaggtta ctggaatagg atcgaagaat
     1261 atgtagagcg gcattcgggc gcgcaattta cacacggtat ttgccctgaa tgctcagtca
     1321 aactctaccc tgacctttaa cgaaggtttg tggcgtattt aatgcttttt atgtccccac
     1381 attttttcgc cggcttttat cggtatcggc agtttgtttt gtagaggcgg taacggacag
     1441 gtagcgtaat ccgtaaatac gcagggcggg ttataggctt tattaaaatc tatgatgacc
     1501 gaatcacctt gagccggcat tttggtgtaa agaaaacgac cgccaccgta agtttccgat
     1561 ccgttggtgc ggtcaccgaa aataatgaat aagtcatccg aaccatcttc caataccggt
     1621 tcgagtgtat aggtttgatc atctatggta aacgtcaaaa gcccggggca tttcatcgaa
     1681 tccacggttc ccaaaatggt aggtattaga attattttca tcggatcgta ttttttaatt
     1741 gtggcatgcg tttgccagtt cttatcaacg gtgtaacgat caatgccttg aaacgatttt
     1801 agcaaagggc tttctaaatc tttaagacga atggcgtatt caccatttcg tttgatcacg
     1861 taaaaacgaa aggaacccat cgccaggcgc gtcattagtg agtcagagtc gtctttcaaa
     1921 acggaagtag aaattgtttt gccgtcagcg gttatgacga catctttttt tacagttaat
     1981 gtgataccac ctttttgaag agtaattgtt ccggcaaacg gaggcgcctg cggaggaaaa
     2041 ataagctgat tggaagaatc gcttccgaat gtattatcgc cctctttgag ccaatgcaat
     2101 ccgactaagt tgagccatcc tttttctttt ttcagattgg cttcccgttg tgcgtgccat
     2161 gcgttgatct cctgtacgta agcggcatcg gttatgatcg gttctttctt tttttggcag
     2221 gcgcatatga aaagaagcag tatggacaat ccggtaaaga gaaaaaactt acctcgtttc
     2281 ataagcgaca tcctttttga gttggcgtcc gagtgcgatg gcgttttcga tgttgcgatc
     2341 caatatgtta aagtgcccca tttttcgtcc cggccgggct tcagattttc cgtacaaatg
     2401 aagttttacg tgcgtttgtg tgaaaagttt ttcccagtgc ggttgctgat ttccccaaag
     2461 gtcaccgaga aggtttacca tcacaacagg agataataaa gaaggatcgc ctgccggtaa
     2521 accgcaaagt acgcgtactt gctgttcgaa ttgatccgta atgcacgcat ccagcgtata
     2581 atgtgcgctg ttatgaggac ggggcgccat ttcgttgacc aatacgcgcc catccggcat
     2641 gacaaacatt tcaacggcaa gcacgccatg atagtccaat ttttcagcga cctgtcgcgc
     2701 gatggtgcgt gcctgactat ccattgtccc cgcgatacgc gccggcacga ttgtcatatc
     2761 taatattccg ttttcgtgta tgttttcgcc ggtgggatat tctgtgatgc ttccatccac
     2821 cgcgcgggct agtacaacgg acacttcttt ttcaaaattg agcatttcct caagaatgca
     2881 cggtgcgtca tgcatggcac gaaaagcatt acggacatct tggaccgtac gaacacggta
     2941 ttgccctttg ccgtcataac cgtaacgatt acgtttgaga attgccggca gtccgacaaa
     3001 gtgtacggct tcatctatat cggactctgt gcgtatcgca gcaaatttgt tcgtcgggat
     3061 ttgatgaata cgtaaaaatg atttctcgtg caatcggtct tgcgtaacgg aaacggcttt
     3121 agcggatggg cgaataaagc agtgatgcga taatatttca agtgtgtcgg cgggtacgtt
     3181 ttcaaattcc gtagtaatgg cagagcactc cgtacgtaaa atatgaagcg cttcagcctc
     3241 gtgataatcg gccgtgagat ggcgatctgc gatatgtccc gccggactct gcggatccgg
     3301 gtcaagcacg atgacgcgat atcccatcgc atgagcggcc atagtaaaca tgcgtccaag
     3361 ctggccgccg ccgagcatac caagtgtaga gccgggttta atcatgcatt ataaatctta
     3421 agaagttttt aaatggatgc gtactgcaat tattttggca acttgagttg taaaactttt
     3481 cgagtctggg attcgcgaaa agcagaaagt tttttcgcta aagtgtcgtc cgtcgttgcc
     3541 aagatggcta tggcctgtaa agccgcattt gcggccccgg attcaccgat cgcaaacgta
     3601 gccacgggaa tacctctggg catctgtacg atcgaaagaa gcgaatccag tccttttaag
     3661 tatcgcgaag gtacgggcac ccctaaaacc gggacaatag ttttggaagc taacatcccc
     3721 ggtaaatgag ccgctccgcc ggctccggca ataatacacc gcaatccccg ctcttgagcg
     3781 gaggccgcat aggaaaaaag aagttccggt gtacgatggg ctgaaacaac acgcgcttca
     3841 taagggattt tgaaagtctt taactgttct accgcatatt tcatcacttc ccagtcgctg
     3901 tcgctcccca taaccacgcc aataagaatt ttttgagcca tgatattgtg tttctgatat
     3961 taaacttgtg ttgatgatac gagtttcatg cataaccctc aagttttaaa gcaatttccc
     4021 ctgattgtaa tatgtcaatg tacaggaaac tcatttaaca aaatttcgta aaaataatta
     4081 cgtaagacaa tacattttat tttggaaagg ttcgttttat gtttaaaagg gtaattgaaa
     4141 cgtcagatga ttcgatcctg acgattttgc gcgtagtttc ggggtcggtt attctgatgc
     4201 acggcttaca aaaacttttg ggttggtttg gcgggcatgg tgtgacagct acaatgcaag
     4261 cgtttgaagc atggtttggt atgccgtaca tcgtaacttt gttagttatt ttatccgatt
     4321 cgttaggtgc gatatgcctt attgccggcg ctgtaacacg tttcatggcc gcgtccattt
     4381 tatcggtaat gatcggagca atcatactgg tgcacgggcg ctgggggttt tatatgaatt
     4441 ggtatagtga aaagcgcggg gaagggtttg aatttcactt attagtcatc gctataatga
     4501 tcgtactcat tatgcgtggc ggcggtttat ggtcggtaga tcggtggttg gtgaaaaaat
     4561 ttaaagcgac cgataatatc taacagttat ttgcaataaa aaaagcagag ccagtaagct
     4621 ctgcttttat agaagagttg atatcacacg cagttttaga atgcatgaat gagcgataat
     4681 cgtaattcac gggagaactt accgatatct ttactattat tgagaatatc agtcattata
     4741 aattcgacca ttactttttg accaaaattg agtttagcgc cgacattcat ataacctcgt
     4801 ccgtgcccga taatgtcttt attgtcattg agcgcgaggt cgtattccca aacggctgtt
     4861 aactcgtcat tgatgctttt atcaacaccg acaaaaaatg caggttgatt gtctttatct
     4921 ttcttttcag tgaccgacca tgaaatgccg ccgtgaattc ctaaatttcc ggcaaaggca
     4981 taatttttac tggccgtgat aaaaagacca cgcgactttt gcgtgtaacg gtttactttg
     5041 actgtatcaa ccgatttgta ataaggaccg aaaccttggt tgttaaagcc aagtgttacc
     5101 gcggggccga tcgttgattc gtcaacggca cgatatcgta catcaacacc tgggagtggg
     5161 ttaaagttaa ccttgccgga gccgataatt ttttctccac catacgaaat accgatcatg
     5221 aaacgatccg ttatgcctac gcctatatta gaaagcacgc caccattagc gaaaagtcgc
     5281 attccgattt cgtaattgcc tttgttgagc atgcctgcgg tcggtacgtt aacaagctgc
     5341 cgatgctgat aaggcgtagc cgggttcgat tcttgagcaa aagcagcaga tacagcacat
     5401 atgaagagca aaataatttt tttcaagtga gttcctccgt ttctgttttg tgtacattcg
     5461 tgcaaatgtt tatacgtaaa tgtacaaaaa agttttaatc acgttttcgt ttttggcgat
     5521 tcaaaaacca acgctcacca ccggtaaaag ataatgtcaa gcgataaacg cggtcacgga
     5581 ctttattatc cgcgatttgt ccgcgctgcc ctgcctccaa gctcaaatca aaactgcttc
     5641 gttcgcggtt aaacggcatc ccaataccca aagtaaacat tatttcgctg gcgtgatttc
     5701 ctgtcgtatt ggttgcatac aaattgttaa tcattaggcc gaagcggtaa taattcttat
     5761 cccaccaggg ggcagaaaag gcgttcgatg gttgcctttc gataccgctg gaccaacgaa
     5821 cggtgtttct atttttttgt gtgggactaa attcttttac cgatccccat gttccgtaaa
     5881 cgatatccgt cgccaataaa taacgcttag tccatcgata agacgcgcca aatccgattt
     5941 ctgtagggta attcaaattt tgttcatagg atacagtcgt atcgcggtaa gtaccgacca
     6001 cacgtatatc gctggatatt tttccggcca tcgacagaaa tccgccaaaa ttccattgct
     6061 cattatgttg ataaaggcct ccgacgacca atcgaccgcc gttcatgttt tgatgtctta
     6121 cgtcgcgcgt atcaactaat tcatccgagc tatagttaat gtcccattct ctttttatgg
     6181 tgccgaagag atactgcaac gatgcgccta tggtccactt gggattcagt ttgcctgctc
     6241 cggtaaaggc aagttgttga agcccacctg ttccgattaa agtttcggaa tatttttcac
     6301 cttcaaagat caaattcggt gttgaaaatt cgtaattgac atttgttacg cgcgagagat
     6361 taatcgctgc gactaacgtc tttccaaagg gaaacgcaaa tgccacatgg ttgatcgtag
     6421 cgcggttgga gaaatttttg gaagcgccgt tactggcttg attccattcg gcaaacatgt
     6481 ttagctcaaa acgtgtcatc ggaatcccga gtaacgtggc ggggttttgc atgttaagcg
     6541 tatagtaact tggaatggct aaacctgctc cgcccatgcc gagcgatctg gggttcggtt
     6601 tgtattcgta tagtccataa gtggtcgcag aatatgaaga accgcctgca taagcagtcg
     6661 aagttgccaa atttgcaaaa atcattaatg ttagaatagc ggtatatcgc ataatgggct
     6721 tcttttattg cggtgactga gtatagtaaa tgattatttt tggagaatta tcgcgatcgg
     6781 acggattatt ataaaaacgg acgcgatata atgggttaaa attaccgttg aatatgttat
     6841 agttcatcaa taccaagccg ttattgtggt ttggttgggt tgcccaatct tgaacaaacg
     6901 aggaaatatt cagttttaat gtgtcatcgc gaatattgtc ggtcctcgaa ttcgttgtgg
     6961 tttgctcaat ataccccggt ttccataagc tggtatctac ggtggaagcc atagcttgaa
     7021 tatttatcga atcgctgcta ttacggaaat aatttccaga agcctcagtt ttcagtacta
     7081 atacggcgga gtttatcgtt aaatccgatg gaagcttatc tgtattaaag cgaatcaaag
     7141 aacggtatgg tacgcctccg ccaacagtaa tggaattcaa tgcttgcggg ttgcgatcac
     7201 gataaacgta tccgtggtag ccgccgttat aaacaccgga aataaaaacg ggagagacat
     7261 aaaaagtact acggtagttt ttatcgtcag ggtcctcgta atccccatca ccatttttat
     7321 cgcgcaaata cacatactcg acttctattc ggggtaccag atcaaaatca atgattgttc
     7381 cattgattgt aataatggta ttgttcccac tgtaaaattg ttgcgtaaaa ggggctccgc
     7441 catcaaaatc aatgatgatg ccgttatttg ttttagggtt tatcgtactg gtgtcgtttc
     7501 ttgctttgac ccattgtact acactatctt tattaagtgt tatttccaac gaatctttgt
     7561 catttaattt tgagaagtta atcgttccga ttgagttggg gctataatct gtaccttgtt
     7621 gaaatttatg ccataataaa tccagttcac tccaaggtgt ttgaacggtg tggatgtgcg
     7681 cattgatact accagcagag ccgaatgcat cggaagcgac gagttgaagg cgtgttttga
     7741 caattatcaa actgtcaagg ttgatgagtt ctccgctggg atccgtaatg tagtcagatg
     7801 ggaaagcaaa acgcattaga attcgaaaat cataattatc tactgaaccc aaatacaagc
     7861 gagaggagtt ataagtcgaa atcgaatcaa caaatgaagc aatccgttcg atatcatcgg
     7921 catataatgt atctgttttg aaagttccgg tagaggtacc gcggtcaata acttccatgc
     7981 cgacgtcttt taggtggtca tcgcatgata taagagaaac gagcagaaat aaaaagccat
     8041 gaaagatgtt ttttgaaaac gaagcgataa agaaactgcg caaaatgaaa tttctcccgt
     8101 actttaaaat ttaatgaaaa tgcggtgacc gcagaattta caattggatc cgcgcatacc
     8161 cccgagattg gattggtaat cgcggcgttt gatgatcaga cgtttacagt tgggacaaaa
     8221 tgtatccgag acaccgtcaa tgtccgtatc cgtcaaatat acataactca gcttttgtgt
     8281 ggccagtttg tatgttcgca ccatcgtttc attgggtgtc atgggttgcg acgtcatttt
     8341 atggtgcggg aaaaaacgtt taaaatgtac gggtatgtct ttattgatgc ccgcaacgaa
     8401 atcaacaaaa ttacccacat cttccgtact gtcggattca ccggggatca acaagtacgt
     8461 caattccaaa tgcgtttttc cgaatgcgga tttgatggaa ttgagtacga tttcaagttt
     8521 actttccgtt aatcgttcat gtgtttccgc gttcatagat ttgacgctga tgttcatcgc
     8581 atcaatgtaa ggaataatgt cttcaatagg tttttgattc aaatatccgt tggtaatcaa
     8641 aatatttttc tgtccgatac ctttcagctt acgcatagag tcaatcagaa attcatacca
     8701 tatcaaaggt tccgtaaacg tataagccac gccgatggaa tggctttttc cggaataatc
     8761 cagaatcgtt tgcagagata tatcttttgt ctctttttta ttcaatgcca catcgtggtt
     8821 ttggcagaat ttgcaacgca tgttgcagcc gttaggtgcc aacgataaaa tggtggaatt
     8881 ggggtaataa tggaaaaatg gttttttctc catcggatcg taaacggccg taacgatttt
     8941 accgtaatta aaagagtaca atttgccgtc aatattctta tatccccagc agtcgccctc
     9001 cgccccctcc tgtattttac acatacgagg gcatagttca cagacgatat gatcggcttc
     9061 tttacggtaa aatttcgctt cgatcatgat atagttccgc ttcaatccat taagtaagta
     9121 ctttgaaaaa caatatattt acactaaatt aaaaatgcaa tttttaatcc tattgttttt
     9181 ataaggcttg tacgtgtatc gacggttaga tgttcgtgct caaaaaaagt ttaatgcctg
     9241 attttaatcg aataacgtat ttttttcctc caagctaacg cttgatgcat ggaaacttaa
     9301 gttaacggta gatagttatg tcctaccaat tcaaacatct cacaccacaa acgtggaatg
     9361 attttgccgc gctgtttggt gaacgcggcg catgtggagg atgctggtgt atggcatggc
     9421 gccggtctaa cgccgagttt caaaaaaata aaggcgaagg caatcgcaaa gccatgcagc
     9481 gattggtaaa aaaagaatta cctatcggtt tgatcatgta taaagatgtt attccggtgg
     9541 gatggtgcgc atttgccccg cgggaggaat atatacggct tgcggcttca cgcattttca
     9601 aacccgtcga tgaaaaaccc gtgtggtcta tttcatgttt ttttattcac aaagattatc
     9661 gtaaacaagg tttgtcggaa aagctcatcc ggcaagtcat cgtatcggct aaaaaaatgg
     9721 gtgttcaaat tttagaagcg tatcccagcg acccgtatga tgctcaagtg cctgcgccat
     9781 ttgtatggac agggctgact tcggcttttg tcaaagccgg gttcaaagaa gttgcacgca
     9841 ggtccaaaac gcgccctatc atgcgctacg ttgtaaatac gaccgactta aataaataaa
     9901 ataaaacagg tgaagaatat gatatttttg ctctttttta tttttttagc ggttcaatct
     9961 gctgcaacac aagatacatt gccggagttt cccgttcatt ttgtggtgat tgacggatcg
    10021 gaagaagtgg ccgctgttgc gacattggaa caaatgcatc gcgagattga gatactgaat
    10081 acttatttcg ttaccgaaga taaacggacg ttagtgcgtt ttcgatttaa atcagcgact
    10141 ttttttgatg agatcgaaaa cacggcgtgt tcgtttgttg attccgctaa tgagggcatg
    10201 tatatcgggc gctgggccaa tttatataaa gaatgtgccg atacgaaact gcgcgatccg
    10261 cgagcgatta actttgttat ttttgattct tacacggatg cccaaggttg gcggtcgcga
    10321 cgaagccgcg gtgcaaccta tgccggcgtt ccggtggtgt ttattgatta tcaggcgctc
    10381 ggccacaaag gttcgctgga agagcatgag atgggacacg cgtttggttt aggtcatatt
    10441 tgcgattcga ctttgacgga agacggcggt caaaatatca tgcatcataa cggcgaatgc
    10501 cccgatcaaa aaaaatatcg tgcgaaatat tacaccggtt ttaacgccga gcaagaagcg
    10561 attatccgac gagctatcct tcgtcatcgc aaaaaattag gattagataa ataatgcctc
    10621 tctcgccgaa gatcgatcgt attctttcaa cagttctgtt tttgggattg tgtgtcttat
    10681 cgttttggat gatcggttgg tttcagccca accatgacta caaagcaaaa gccgtgattt
    10741 acaaaccttc tatatttgta tatgaagc
//
LOCUS       DAOQNQ010000086        10708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2209_length_10708_cov_4.678213, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000086 DAOQNQ010000000
VERSION     DAOQNQ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10708
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2209_length_10708_cov_4.678213"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..1406)
                     /locus_tag="PK754_07100"
     CDS             complement(189..1406)
                     /locus_tag="PK754_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HNB09307.1"
                     /translation="MDLNTLIYDWNQEVNAEEKPAHAFELNDETLRDGLQSPSVKTPT
                     LEQKIEILHLMEGLGIHAADIGLPGAGPHVLEHVVALAAEIAKNKFKIQPNCAARTLK
                     VDIDPIIEASQRTGIPIQACTFIGSSPIRQYAEGWGMDQLLKFTEEAVSYAVKNNLDV
                     MYVTEDTTRAHPNDVKRLYLAAIRSGARRVCISDTVGNATPEGVQRLVRFMVEVIKES
                     GEDVKIDWHGHNDRGLAVANAIIALQAGAHRVHGTGIGVGERVGNVQMDQLMVNLKLK
                     NWITNDLTTLPQYCRKISEYTGIPIPINYPVFGHDAFRTGTGVHAAAILKSLNKGDME
                     LVDRVYSSVPAAMFGLEQVVEIGPLSGESNVVFWLKRRKIEPREELVKKIFAAAKQSQ
                     TVLSDEQIFSLCR"
     gene            complement(1458..3152)
                     /locus_tag="PK754_07105"
     CDS             complement(1458..3152)
                     /locus_tag="PK754_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNB09308.1"
                     /translation="MASLVLIENNTTATRFRIEKDDTIIGRRPENDIMIANVMISGKH
                     ARVEKKNGLYQITDLGSTNGTFVNGARVTGSAEIKNQDHINFGAVELIFMTDENAMVN
                     IDRTRFYSRDKAVAPPQKPHDTGYAANPNADKIVQLFQQLKNTVLSGSADRTAIMTQF
                     MEVEHQLQIMNMQFKESEKSQQKLNVLYEIGKVINHILVEEELLKTIIDLALKVMNGD
                     CGFIMLYDDKNNLVPRVSRKMQADEISQSGSTFSSTIAKQVVESNQSILTSDAKSDSR
                     FQSGASIISNNIRSVICSPMRNKDQAVIGVIYVGSNVMTNVFSKSDVELLEAFANHAA
                     ISIENAKMHEERRRKEHLKSALERYVSKQIAERIMSNDGSSIRFTPERREVTLIFSDV
                     RGFTTLSEKLSAEEMVEILNRYFSRMIDVIFKYGGTLDKFIGDSIMALFGAPATSGDD
                     AWNAVQAAIEMQRGLEAFNDEQRQLGKPEIQVGIGINTGHVVVGNIGSDQRMEYTAIG
                     DNVNLASRLQGKAAGGKIIISKATYDFVKDKVQATLLGTTEVKGKTIPVEIYEVMY"
     gene            complement(3171..3890)
                     /locus_tag="PK754_07110"
     CDS             complement(3171..3890)
                     /locus_tag="PK754_07110"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB09309.1"
                     /translation="MKKHFSLITLLFAGIIIFTGCGGSKNTTKEKPETPAVPKKPSGK
                     PQLEKGMELYKARDYEGAKKNLRSATDLDLSKSERTQTYKHLGFIFAMQKNQTEATKA
                     FFDAFTSDGEFELESAEMGNPAWTPAYEAAQKQFTLASASGSDLLSKGKDAYSKRNYD
                     EALSYLETAVKKTDLEGNKRADAYKLLAFAYALRKRPVDAKNAFRKAFEANKNFELDK
                     SEYGNPVWTPLYDEIKNTIKK"
     gene            complement(3892..6102)
                     /locus_tag="PK754_07115"
     CDS             complement(3892..6102)
                     /locus_tag="PK754_07115"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNB09310.1"
                     /translation="MEPKKLGRYEIVQVLGQGAMGIVYKGVDPMIGRTVALKTLKSGS
                     EIPEQQLTEFKRRFAQEAQSAGRLNHRNIVTVYDVGEEEGLAYIAMEFIKGRPLDDLI
                     AEKHPFTIEQIVDIMIQICEGLGYAHKNGVIHRDIKPANIVLTDDQIAKVTDFGIAKL
                     TSTSATQTGMVVGTPSYMSPEQITGRGVDNRSDIFSIGAVFYELLTYEKAFPGDNITT
                     VMYRVVHENPAPLSISNAMVPPQFQAIIERAMAKNPADRYPDAETMASDIRNYKTATA
                     NMGATQVINPANYAGATVVMSAPDFSAMSPTGTGSMPSPAAAQTQSIPAASPASKKSP
                     VALIAGGAVAAILIVLYMMFGGSGDKSSASSAKSSGTGNASLTLTLNAAEGTAMLDSV
                     HVPIKDAKISAEKITAGEHELVIQSDGYETYRSKLLFADGETKQFAVELKLAPVKFPA
                     GVDTAYITVSSTDMIKVMTNTGTMLGYSPIQRLPFPSGKHTLVFSKPFYTNQVSEIDA
                     RKGKEIKLTPKLQAKRVKANLAGVEPKKIQVYLNSVASESQLKPEKDGVTYSLPLGTN
                     KLIVTADGYKSYEQELNLGDDAIPFDVKATLVQGFGSLAITSKPDGADVYVDGKKEGV
                     TPFKMDKIPAGPRDIKVQKGSLLSIKKMNIEADKENAADFTLAASTGILKILINPWGN
                     ISVDGKPMGASPPLERLELSPGNHTIKIENPAYKAVTKQVKITVGQTTTLQYDF"
     gene            6316..7452
                     /gene="thiO"
                     /locus_tag="PK754_07120"
     CDS             6316..7452
                     /gene="thiO"
                     /locus_tag="PK754_07120"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HNB09311.1"
                     /translation="MKIYDVVVIGGGVIGLSIAYEIKFRHNKIKVAILEKTQTGYEAS
                     HASAGMLEPQLIMPRQNLSDREKAFFRLCRESQNMYEETIRRIEYHSRMDCEYRKEGI
                     LKLISPETDPKPWLSWYVGLGMHAHFWSVEQTGEAEPALREGMAAIHLPDNHQVENRK
                     LSAALAECCKNLGVDILEYSAVESIILNNEAIDHLQTKTGTVSAARYVLAAGAWSSQI
                     PGLTQTIPEIKPIRGQIVAMQMPKPDFIRYPAYLEDFYYVPRNSGRLLIGSTVEEAGF
                     DKAVTPSVIVGFIEKLRALIPQSKTFESVERWAGLRPCSADRLPLLGETRLTNLIVAT
                     GHFRNGILLMPITAKLIADMIITGKSSPMLAPFSPSRFNGSTNA"
     gene            7449..7943
                     /locus_tag="PK754_07125"
     CDS             7449..7943
                     /locus_tag="PK754_07125"
                     /inference="COORDINATES: protein motif:HMM:NF025046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HNB09312.1"
                     /translation="MNIRNYTWRGAILLFAGAWIGILVFLAYGVAGTIFRNLDSKTIA
                     GYINGLILVRMNHLEYLCAALIVVSSFVLYRRQKTLWSRVRGVVSGVMLINLFFYADF
                     ITPQMTMLKEKIVDFDQYAKENDPRPERKEFDAWHRRYSALVGFNIVLALWVVGLTLR
                     ERDE"
     gene            7936..8829
                     /gene="hpnC"
                     /locus_tag="PK754_07130"
     CDS             7936..8829
                     /gene="hpnC"
                     /locus_tag="PK754_07130"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HNB09313.1"
                     /translation="MNEERAIGSYSVDEIDAAYAMCRDFAASHYENFPVISWIFNAAQ
                     RRHLAAVYTFLRRADDVADEGTGTIEQRMHGLLWMQAALKASEEGRAKDPMFAALSVT
                     LQKGGIPRQWLDDVLSAFKVDLLKNRYTDEEQLMGYCRYSANPVGLIVLHILGYKYNS
                     TMWEKMTPLSDAICSALQLTNHWQDVFIDLQKNRLYIPLELMKECGYTEEDWKNKTVN
                     PSFSKTMAVCLKKTRDLFDQGHALLGYLRFKDRKIIGMIWLSGLRVLMKMEKTGGDTL
                     NHRPTIGLTDKFWILWRVLWI"
     gene            8820..9647
                     /locus_tag="PK754_07135"
     CDS             8820..9647
                     /locus_tag="PK754_07135"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HNB09314.1"
                     /translation="MDIETILQEDARSITRRSRSSFYYPMMLFPGGKREAIFTVYAFC
                     RITDDIVDQNGSDAATHLSVWKKELIAAERGYSSYYLLNRVMRVARDFSIPVQLFHDV
                     IRGMEMDLERREYRTFDELYEYCYCVASAVGLMIIRILGAHHTASERFALHAGLALQL
                     TNTIRDAASDIADGRIYFPVTDRRAFFDNPSAVQDALRLQCAEARKQFQLADEAFRAY
                     PSLALFPARVMENMYRLLLDKIEQTGFTPTAHRVSLTLSEKIQTAGRTWFDDRPYRG"
     gene            9625..>10708
                     /locus_tag="PK754_07140"
     CDS             9625..>10708
                     /locus_tag="PK754_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB09315.1"
                     /translation="MIALTVAKSPSQIIIIGAGTAGLAAATTLAQAGYDVSLYEKNAY
                     AGGRTFSFTDVASRSTLDNGQHLLMGCYHATRAWLDSLNASLDIPKKSFPLNFYSADG
                     RIACLSLPDWPAPWYALYGIMRYDAIPLSERWRILRAGLSMRKSLIRGRYVSDWLDAW
                     RFTQDTRRWFWNPICLAIMNQHPDDADLVQFGEAMRRIFFGSRDDGRFIISEKSLDAV
                     LVEPAVRFLKQKSCSLHFHSFVRHITVSNGRVEGITLRNGETIRTDIVIMAVPPWSAG
                     KLLPVENEWTSRLQNFSSAPILSAYIWLNEMHPDALFDGPMAACIGTHVQWVFKKNSE
                     CIQITISHPGASVFDAASIWEKRITDD"
BASE COUNT         2596 a         2395 c         2685 g         3032 t
ORIGIN      
        1 gtgtgcggat tttgttttag ccgccgttcc aaagcccgtg gcgaaatatc cggtatgacc
       61 gtggcgtgca ggtagtctgg tttatcttta aaaaagatcc aatacgtctt ttcggtcgtt
      121 tgaccccaaa gcgtgttgct tatcaaaaaa ataaaaagac ttacgcgaga caaaatcatc
      181 cccaaccttt atcggcaaag cgaaaaaatc tgttcgtcac tcaaaacggt ttgcgattgt
      241 tttgccgcag cgaatatttt tttaaccaat tcttcacgcg gttcgatctt gcggcgtttg
      301 agccaaaaaa ccacattgga ctcaccgctc aacgggccta tttctacgac ttgttcgagg
      361 ccaaacatag ccgccggtac gctggagtac acgcgatcca ccaattccat gtcaccttta
      421 ttgagggatt tcaggatcgc cgcggcatga actcccgtac cggtgcgaaa tgcatcgtga
      481 ccgaaaacgg gataattgat gggaatcggt atgcccgtgt attccgaaat tttacggcaa
      541 tattgcggca gagtagtaag atcgttggtt atccagtttt tcaacttgag attgaccatg
      601 agttgatcca tttgcacatt gccgacgcgt tcgccgacac cgatgcccgt tccgtggacg
      661 cgatgggctc cggcttgtaa tgcgatgatt gcgttagcga cggcaagccc gcggtcattg
      721 tggccatgcc aatctatttt tacatcttcg ccgctttctt tgattacttc caccataaaa
      781 cgtacaaggc gctgtacacc ttcgggggtt gcattgccga cggtatcgga gatacaaacg
      841 cgccgtgcac cgcttcgtat cgcggcaagg tacaggcgtt taacatcgtt gggatgagca
      901 cgggtcgtgt cctccgtaac gtacatcaca tccagattgt ttttcacggc gtatgaaacg
      961 gcctcttccg taaatttcaa aagttgatcc atgccccaac cttcggcgta ctggcgaatg
     1021 gggctcgagc cgataaacgt gcacgcctga atggggatac cggtgcgttg ggatgcctca
     1081 atgatcggat caatatcaac cttgagtgta cgtgctgcgc aattaggttg gattttaaat
     1141 ttattttttg cgatttcggc ggccaaagca acaacgtgtt cgagtacatg agggccggcg
     1201 ccgggcaaac cgatgtcggc ggcatgaata ccgagtccct ccatgaggtg caagatttca
     1261 attttttgct caagtgtagg cgtttttacg gaaggcgatt gtaaaccgtc acgcaaagtt
     1321 tcatcgttga gttcgaaagc atgcgcgggt ttttcttcag cgttgacttc ttgattccaa
     1381 tcgtaaatca gcgtattaag atccatatat agtttctttc aaatagatgg cgatatagtt
     1441 tagactttac gaagatatca atacatcact tcgtaaattt cgacaggtat ggttttgcct
     1501 ttgacttcgg ttgttccgag aagagtggct tgcaccttat ctttgacaaa atcataagta
     1561 gctttgctga tgattatttt tcctccggcc gctttgcctt gaagacgtga tgccagattg
     1621 acattatcgc cgatagccgt gtattccatg cgctgatcgg atccgatgtt gccgacgacg
     1681 acgtggccgg tgttgattcc gatgccaact tgaatttcgg gtttgcccaa ttgacgctgt
     1741 tcgtcgttaa atgcctccaa accgcgttgc atctctatcg cagcctgcac ggcattccat
     1801 gcatcatcgc cggaagtggc cggagctccg aaaagcgcca taatggagtc gccgataaat
     1861 ttatccaacg tgccgccgta tttgaaaatc acatcaatca tacgcgaaaa ataccgatta
     1921 agtatttcaa ccatttcttc ggcagataat ttttccgata gggtagtgaa accgcgtacg
     1981 tcggaaaaaa tgagcgtgac ttcgcggcgt tcgggcgtaa aacgaataga actgccgtcg
     2041 ttactcatga tacgttcggc aatttgtttg gacacataac gttccaaggc gcttttgagg
     2101 tgctctttgc gacggcgttc ttcatgcatt ttggcgtttt cgatggagat ggccgcgtga
     2161 ttggcgaaag cctcaagcaa ttcaacgtcg ctttttgaaa atacgttggt catgacattg
     2221 gagccgacgt agatcacgcc gatgacagcc tgatctttat tgcgcatcgg tgaacagata
     2281 acggagcgaa tgttgttgga tataatactg gcgccgctct gaaagcgcga atcgcttttg
     2341 gcgtcggaag tcagaatgga ttgattgctt tcgaccactt gtttggcgat cgtcgaactg
     2401 aacgtcgaac cggattggga aatttcgtcg gcctgcattt tgcgcgaaac gcgtgggaca
     2461 agattgtttt tatcatcgta caacatgata aatccgcagt cgccgttcat cactttgagg
     2521 gctaaatcaa tgatcgtttt cagcaattct tcttcaacga gaatgtggtt gatgactttc
     2581 ccgatctcgt agagcacatt gagtttttgc tgcgattttt cggattcttt gaactgcata
     2641 ttcatgatct gcagttgatg ttcgacctcc ataaactgag tcatgatggc cgtacgatcc
     2701 gccgaaccgc tgagcacggt atttttgagc tgttggaaaa gttgcacgat cttgtcggca
     2761 ttgggattgg ccgcatagcc cgtgtcatgg ggtttttgcg gcggcgcgac agctttgtcc
     2821 cgggagtaaa aacgcgtccg gtcaatattg accatcgcat tttcatccgt cataaaaatg
     2881 agctcgacgg cgccgaaatt gatatgatcc tgattcttaa tttcagcgga accggtaacc
     2941 cgtgctccgt tgacaaatgt gccgttcgtg gagccgagat cggtgatctg atacaagccg
     3001 ttcttttttt caacacgggc atgtttgccg gaaatcatca cgttggcgat catgatatcg
     3061 ttttccggtc ggcgtcctat gatcgtatcg tctttttcga tacgaaagcg tgttgcggtt
     3121 gtgttatttt cgatgagaac gagagaagcc acccgtacgt ccaatctgta ttattttttg
     3181 atagtgtttt ttatttcatc ataaagcggc gtccaaaccg gatttccgta ttcggattta
     3241 tccaactcga aattcttatt ggcttcaaag gctttgcgaa atgcgttttt agcatctacg
     3301 gggcgcttac ggagagcata ggcaaaagcc aatagtttgt aagcatccgc acgtttgttg
     3361 ccttcgagat cagttttttt aaccgctgtt tccaaatacg atagcgcttc atcgtagtta
     3421 cgtttagaat aagcatcttt gcctttactc agcagatcgc tgcccgaggc gctcgctaac
     3481 gtaaattgtt tttgtgccgc ttcgtaagcc ggtgtccagg ccggattacc catttcagcg
     3541 gattccaatt caaattcgcc atcacttgta aatgcgtcga agaatgcttt agtggcttcc
     3601 gtttgatttt tttgcatcgc aaatatgaaa ccgagatgtt tatacgtttg ggtacgctcg
     3661 cttttggata aatcaagatc ggtagcgctt cgcagatttt ttttagcgcc ttcgtaatcc
     3721 cgggctttgt atagttccat tcctttttcc aactgcggct taccgctggg ctttttggga
     3781 acggccggtg tttcgggttt ttctttggtc gtgttttttg atccgccgca accggtaaaa
     3841 attattattc cggcgaaaag gagtgtgatg aggctaaaat gttttttcat attagaaatc
     3901 atattgtaac gtagtggttt ggcctacggt gatttttact tgtttagtga cggctttgta
     3961 cgccgggttt tcgattttga tcgtatgatt acccggactc aattcgagac gttcgagcgg
     4021 cggagaagcg cccatcggtt taccgtccac cgatatattg ccccagggat tgattaagat
     4081 tttcaaaatg cccgtggatg cggcgagtgt aaagtcggct gcattttctt tatcggcttc
     4141 gatattcatt tttttgatac tgagcagcga gcctttttga actttgatat cacgcggtcc
     4201 ggcaggaatt ttatccattt tgaatggggt aaccccttct ttttttccgt ctacatatac
     4261 atccgcgccg tccggtttgg aagtgatcgc gagcgatccg aaaccttgta ccagcgtggc
     4321 tttgacatca aagggtattg catcgtcgcc gagattaagt tcctgttcat aacttttgta
     4381 gccgtcggcc gtaacgatta gtttattagt acccagtggt agggaatacg tcacaccgtc
     4441 tttctccggc ttcaactgac tttcagaggc tacggaattc agatatacct gaattttttt
     4501 aggttctacc ccggcaagat tggctttgac gcgtttggct tgcagtttgg gtgtcagttt
     4561 aatctctttg cctttgcggg cgtcaatttc cgaaacctga ttggtataaa acggcttgga
     4621 aaaaacgagt gtgtgttttc cggacggaaa cggtaaacgt tgaatcggcg aatagcctaa
     4681 catcgttccc gtattggtca tcactttgat catgtccgtc gaagaaacag tgatgtatgc
     4741 cgtatctaca ccggccggaa attttaccgg tgcgagtttg agttctacgg caaactgctt
     4801 ggtttctccg tcagcaaaaa gaagtttaga acgatacgtt tcgtaaccgt cggattgaat
     4861 aactagttca tgctcaccgg cggtgatttt ttctgcagaa attttggcat ctttgatcgg
     4921 cacatgcacg gaatccagca tggccgtgcc ttccgccgcg ttgagtgtta gcgtaagcga
     4981 tgcattccct gtgccggaag atttagctga agaagccgat gatttatcac cggagccgcc
     5041 gaacatcatg tatagcacaa tcaagatcgc agcgacagcg ccgccggcga taagtgccac
     5101 cggtgatttt ttggaagccg gtgatgcggc aggtatggac tgcgtctgcg ccgcagcagg
     5161 ggacggcatg gatcctgtgc cggtaggtga catcgcagaa aaatccggag cggacataac
     5221 gacggttgcg ccggcatagt tagcaggatt gatcacttgc gttgcgccca tgttggccgt
     5281 agctgttttg tagttgcgaa tatcgctggc catggtttcg gcgtcaggat aacgatccgc
     5341 cggattttta gccatcgccc gttcgatgat tgcttgaaat tgcggaggca ccatcgcatt
     5401 gctgatggat agcggcgcag gattttcatg cacgacacga tacatgactg tggtaatatt
     5461 atcgccggga aaggcttttt cgtaagtgag taattcataa aacaccgcgc cgatactgaa
     5521 aatatcggaa cgattatcca cgccgcgccc ggtgatttgt tcaggcgaca tgtagctcgg
     5581 cgtgccgacg accatgccgg tttgtgtcgc actggtggaa gtgagtttgg caatgccgaa
     5641 atcggttact ttggcgatct gatcgtcggt gagaactata ttcgccggtt tgatgtcgcg
     5701 gtggataaca ccgtttttat gcgcgtatcc gagcccttcg caaatctgta tcatgatgtc
     5761 aacgatttgc tctatggtaa agggatgttt ttcggcgatc aggtcatcca aaggacggcc
     5821 tttgatgaat tccatcgcga tgtaggccaa accttcttct tcgccgacgt catatacggt
     5881 cacaatatta cgatgattga gacggcccgc ggactgtgct tcttgcgcaa aacggcgctt
     5941 gaattctgta agctgctgtt ccggaatttc gctcccggac ttaagtgttt tgagagcgac
     6001 tgtgcgtccg atcatcggat ctacgccttt gtacacaatg cccatcgcgc cctgacccag
     6061 gacttgtacg atttcataac ggccaagctt tttcggttcc actaaaaaac tcccggaatt
     6121 gtatatttaa aattacaata aatagataaa agaattgcgg attacaacgc ggcaatatat
     6181 ttcttggcta agccaatatc aagcataaaa aacacctaaa accattgaaa attaagcgtt
     6241 tcagtacgcg cccaagcttg attttttaag acaacgcttc tatattgaat tgtcagcatc
     6301 aaccaggagt tagttttgaa aatatatgat gtcgttgtga tcggcggggg tgtgatcggg
     6361 ctttcgatcg cgtatgaaat caaatttcgt cacaacaaaa taaaagtagc cattttagaa
     6421 aaaacacaaa cgggatatga ggcttcccac gcatccgcag gcatgctcga accgcagttg
     6481 atcatgccgc gtcaaaattt gtcggatcgc gaaaaagctt tttttcgatt gtgcagggaa
     6541 agtcaaaaca tgtatgaaga gaccattcgg cgtatcgaat atcatagccg tatggattgc
     6601 gaataccgca aagaaggcat tctcaaacta atttcgcctg aaacggatcc caagccgtgg
     6661 ttgtcgtggt atgtcgggct cggaatgcat gcgcattttt ggagtgtgga acaaaccggc
     6721 gaagcggagc ctgcattgcg cgaaggcatg gcggcgattc atttgccgga taaccatcag
     6781 gtggaaaacc gcaaattgag cgccgcactg gccgaatgct gcaaaaatct cggcgtggat
     6841 atcttggagt attctgcggt cgaatccatc attctcaaca atgaagcgat tgatcatctt
     6901 caaactaaaa ccgggacggt atcagcggca cgatatgtat tggccgccgg tgcatggtct
     6961 tcacagattc ccggattgac acaaacgata ccggagatca agccgatacg gggtcagatc
     7021 gtcgcgatgc aaatgccgaa acctgatttt attcgatacc cggcatacct tgaagatttt
     7081 tattacgtac cgcgcaatag cggtcggttg cttatcggct caaccgtcga agaagcgggc
     7141 tttgataaag ctgtgacgcc ctcggtgatt gtcggattta tcgaaaaact aagagcattg
     7201 atcccgcagt ccaaaacttt cgaatcggta gagcggtggg ccggtttgcg cccctgtagt
     7261 gcggaccgcc ttccgctgct tggtgaaaca cggttgacca acctcatcgt agcaacaggg
     7321 cattttcgca atggcattct tcttatgccg ataacggcca aacttatagc cgatatgatc
     7381 atcacaggca aatcttcgcc gatgttggcg cccttttcac cctcgcgatt taacgggagt
     7441 accaacgcat gaatatccga aattatacat ggcgcggagc gatattattg tttgccggtg
     7501 catggatcgg tattctcgtg tttttggcat acggtgttgc cggtactatt tttagaaacc
     7561 ttgattccaa aaccatcgcc ggatatatca acggattgat tcttgttcga atgaatcatt
     7621 tggaatacct gtgcgcggcg ttgatcgtag tatcgtcctt tgttctgtat aggcgtcaaa
     7681 aaacactatg gtcgcgtgta cgtggggtcg tgtccggcgt gatgctgatc aatctttttt
     7741 tctacgcgga ttttatcacg ccgcagatga cgatgctcaa agaaaaaatc gtagacttcg
     7801 atcaatacgc caaagaaaat gatccgcgcc ccgagcgtaa ggaattcgac gcatggcatc
     7861 gccggtattc tgcactcgta ggattcaata tcgtactcgc gctttgggta gtcggcctaa
     7921 cactgcgaga acgcgatgaa tgaggagcgg gcgatcggtt cgtattccgt ggatgaaata
     7981 gacgcggcct atgcgatgtg ccgggatttt gccgcttcgc attacgaaaa tttccctgtt
     8041 atttcatgga tttttaatgc cgctcaacgc cgtcaccttg cggcggttta taccttttta
     8101 cgccgcgccg atgatgtagc cgacgaaggc acaggcacga tcgaacaacg tatgcacgga
     8161 cttttatgga tgcaggctgc gctcaaagcc agcgaagaag gacgcgccaa agatccgatg
     8221 tttgcggcgc tgtccgtgac gttgcaaaag ggcggtattc cccgtcaatg gttggatgat
     8281 gttttgagcg cttttaaagt agatcttctt aaaaatagat ataccgacga agagcaactc
     8341 atgggatatt gtcgttattc ggccaatccg gtcggcctca tcgtactgca tatccttggc
     8401 tacaaatata actcgactat gtgggaaaaa atgaccccgt tgtcggatgc catttgttcg
     8461 gcgctccagc tcaccaatca ttggcaggat gtattcattg atcttcagaa aaaccggttg
     8521 tatataccgc tggagctgat gaaagagtgc ggctataccg aagaggactg gaaaaataaa
     8581 acggtaaatc cgtcgttttc aaagacgatg gcggtttgtt taaaaaaaac acgtgatttg
     8641 tttgatcaag gtcatgcgtt gcttggatat ttgcgtttca aagatcgcaa aatcatcggt
     8701 atgatatggt tgagcggatt acgtgtgctt atgaaaatgg aaaaaacggg cggtgatacg
     8761 ctgaatcatc gtccgacgat tggtttaact gacaaatttt ggatactatg gcgcgtttta
     8821 tggatataga aacgatattg caagaggatg ctcgaagcat tacgcgacgc tcccgcagca
     8881 gcttttatta tccgatgatg ctctttccgg gagggaaacg cgaagctatt tttacggtat
     8941 atgcattttg ccggatcacg gatgatatcg tggatcaaaa tggttccgat gccgcgacgc
     9001 atttgtctgt atggaaaaaa gaattgattg ccgccgagcg cggttattcg tcatattatt
     9061 tgctcaatcg ggtgatgcgg gttgcgcgtg atttttcgat tcctgttcaa ttgtttcatg
     9121 acgttattcg cgggatggaa atggatttag agcgcagaga ataccgcaca tttgatgaat
     9181 tgtatgaata ttgctattgc gttgcgtcag ccgttgggtt gatgatcatt aggattttgg
     9241 gagcgcatca tacggccagt gaacggtttg cgttgcatgc cggtttagct ttacaattga
     9301 ccaatacgat tcgtgatgcc gcatctgata tagcggacgg acggatctat tttccggtga
     9361 cggatcggcg tgcgtttttt gataatccgt cagccgttca ggatgcgttg aggctacaat
     9421 gcgccgaagc gagaaaacaa ttccaattgg ccgatgaagc gtttcgtgcg tatccgtcgt
     9481 tggcgctttt tccggcacgg gtcatggaaa atatgtatcg gttattgctt gataaaatcg
     9541 aacaaaccgg ctttacaccg accgctcatc gagtaagcct tactttatcg gaaaaaatac
     9601 aaaccgcagg tcgaacttgg tttgatgatc gcccttaccg tggctaaatc tccctctcaa
     9661 ataatcatca ttggcgccgg tacagccgga ctggctgccg cgacgacact tgcacaggcg
     9721 gggtatgatg taagtttata tgaaaaaaat gcctatgccg gagggcgtac tttttctttt
     9781 accgatgttg catcacgatc gacactcgac aacggccagc atctcctgat ggggtgttat
     9841 catgctaccc gtgcgtggct tgattcatta aatgcctcat tggatattcc caaaaaatcg
     9901 tttcctttga atttttattc cgcggatggc cggatagcct gtttgagttt accggattgg
     9961 cctgcgccgt ggtatgccct gtacggtatt atgcgatacg atgcgatccc attgagcgaa
    10021 cgctggcgta ttttgagagc gggtttatca atgcgtaaat cgttaataag gggacggtat
    10081 gtatccgatt ggttggacgc ctggcgattt acgcaggaca cacgacgatg gttttggaac
    10141 ccgatttgtt tggcgattat gaatcagcat cccgacgatg ccgatttggt tcagtttggc
    10201 gaggctatgc gacgaatttt tttcggttca cgcgacgatg gacgttttat tatttcggaa
    10261 aaatcactcg acgccgtatt ggtcgaaccg gctgtaaggt ttttaaaaca aaaaagttgt
    10321 tcgttgcatt ttcattcatt tgtacgtcat atcaccgttt ctaatggccg ggtagaagga
    10381 atcacgttgc gaaacggcga aaccattcgg acagatatag tcattatggc cgtaccgccg
    10441 tggagtgccg gcaagctttt acctgtagaa aacgaatgga cttcacggct ccaaaatttt
    10501 tcatcggcac cgatattatc ggcctatata tggttaaatg agatgcatcc ggatgcgcta
    10561 ttcgacggac ctatggccgc atgtatagga acgcacgtac aatgggtatt caaaaaaaac
    10621 tcagaatgta ttcagattac gatcagccat cccggcgcgt ctgtttttga tgcagcgtca
    10681 atttgggaaa agagaataac agatgaca
//
LOCUS       DAOQNQ010000087        10698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2212_length_10698_cov_6.132106, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000087 DAOQNQ010000000
VERSION     DAOQNQ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10698
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2212_length_10698_cov_6.132106"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="PK754_07145"
     CDS             complement(<1..1005)
                     /locus_tag="PK754_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNB09316.1"
                     /translation="MKSSRSKNSPVTLLIGTRKGAFTLKSDARRRTWKVSAPIFLGHI
                     VHHLMQDPRESKCWLMACKTGHLGPTVFHSDDRGKTWKESKQPPAFHKTDSAKRPRAV
                     DHVFWLTPGHASEPGVWYAGTSPQGLFRSEDHGVTWKNVDGFNENPMYEKWTGGDKDG
                     TPDGPKLHSIIVDPRNARHMYLGCSSGGVFETADQGKTWTPLNKGCDAEFLPQPDPEY
                     GHDPHCVQLHPLIPDRLYQQNHCGVYRMDRTEGRWYRIGKNLPKHIGDIGFSITLHPT
                     DPDVAWVFPMDGTSVWPRTSPGGKPAVYATYNAGKTWKRLDKGFPKKDAYWTVFRQAM
                     T"
     gene            complement(1057..1710)
                     /locus_tag="PK754_07150"
     CDS             complement(1057..1710)
                     /locus_tag="PK754_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNB09317.1"
                     /translation="MELIAQFIDFFIHLDVHLGELIQNYGTWTYLILFLIIFAETGLV
                     VTPILPGDSLLFAAGAFAGLGYLDPWWLFILLTVAGIIGDSVNYAVGLRIGPKIFHKE
                     NVRFLNKEYLQQAHMFYEKHGGKTIIFARFIPIIRTFAPFVAGIGQMTYRHFIFYNII
                     GSIAWVGLFVSSGYFFGNIPTVRKNFTLVIVAIIIISVIPAVIEFIRARRKSGINEA"
     gene            complement(1729..3090)
                     /locus_tag="PK754_07155"
     CDS             complement(1729..3090)
                     /locus_tag="PK754_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HNB09318.1"
                     /translation="MSFEIQEILETIRMTEVEHFDIRTTTMGISLRDCGRGNASATIQ
                     AIYDKICRYAERHVATAREVEQKYGIAIANKRISITPASIACDGFTRSEFVQLAKKLD
                     EAAERVGVDFLAGFSALVQKGFTKGDRALIESIPEAIGSTKRVCSSINVGSTKAGINM
                     DAIIAVSKVIKDLAQVTASEGAIGCAKFVVFCNAVEDNPFVAGAFHGVTEAECTINVG
                     ISGPGVVLHAIKQAPDADLGELSEIIKRMTFKITRAGELIGREVARQQNIDFGIVDLS
                     LAPTPAPGDSIADILVAMGLEDVGACGTTAALAMLNDAVKKGGLMASSYVGGLSGAFI
                     PVSEDQGMIAAVEKGNLSLEKLEAMTCVCSVGLDMVAVPGDTTWETIAGLIADESAIG
                     MINSKTTAVRIIPVPGKKVGESVDYGGLLGKAPIMPIRNLSPKKFIGRGGRIPAPTVA
                     LSN"
     gene            complement(3221..4009)
                     /locus_tag="PK754_07160"
     CDS             complement(3221..4009)
                     /locus_tag="PK754_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNB09319.1"
                     /translation="MSSEIRELLQAATHAAYEAGKLTLRYYQTASLDVIAKPDASPVT
                     IADRSAEEWLRAFISKEFPGHGILGEEFGETIGSDRYRWILDPIDGTKSFIHGVPFYG
                     TMVAIEDVQKKDAIVGVIHFPALGEILSAATNEGATINGRRVKARTTEYLAEATLLTT
                     DMKSSHQGDYAPILQTLIKETKIVRTWGDCYGHALVACGRADIMVDPKLNLWDIAALK
                     PIIEESGAVIFDRNGVTSMYIDNAIACGPALQKEIWTKLNISHV"
     gene            complement(4083..4523)
                     /locus_tag="PK754_07165"
     CDS             complement(4083..4523)
                     /locus_tag="PK754_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNB09320.1"
                     /translation="MSAKTITSEITIYAQAERVWAVLTEPSLTRQYMFDCEAQSDWKP
                     GSRLEWKSAKDGVVYVRGHIVRNEYPRILEYTVFDPFGGYADIPANYLTVSITLSEKE
                     SATDVSVVQGDFSTVANGEKRYQDSHGGWSYILEKIKTLAEAGT"
     gene            4612..5427
                     /locus_tag="PK754_07170"
     CDS             4612..5427
                     /locus_tag="PK754_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB09321.1"
                     /translation="MTKTNSAGKPRGILSSIPNPRIRYYRDKPTKTLQSTIEHFWMVR
                     WDFPKNRPYIAETLPHPSVHLVFEKDGSSIGGPVTGKFKRTLNGKGFVFGVKFLPGAF
                     SAFYKPSIVNLANHKVNIESVFGESILQLERSIMRMKRFDAMKKAAESFFTSIPIKHD
                     PRAILAQTIVATVMIERDIVHVRQLLARFHITARSLERLFENNIGVTPKWVIQRYRLH
                     EAVEMIKSNAEISLTKLALDLGYHDQAHFIRDFKSLIGMTPSRFLRNATGKTV"
     gene            complement(5424..5924)
                     /locus_tag="PK754_07175"
     CDS             complement(5424..5924)
                     /locus_tag="PK754_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09322.1"
                     /translation="MNHLSDAMMQAYLDGTAGITKQNMETHLSVCEECRESMAIYRMM
                     HHTIRQEEFILPAHFADIVAEKVGLVSADESETSVLREWWWLLPVVAAGIGISAYYLP
                     STMTGAPVERVLQENIRQTQTYFTIAEQMGGWIGSNFQYLIFGLFLVLLFDMIERKFL
                     RLRVQH"
     gene            complement(5921..6538)
                     /locus_tag="PK754_07180"
     CDS             complement(5921..6538)
                     /locus_tag="PK754_07180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB09323.1"
                     /translation="MIRPSMENERLTIEKIIAGDAKAFKQLVEDHQKLVAHIVFKMVS
                     NPSDQQEICQEVFTKVYENLRTFQFNAKLSTWIARIAYNTTLNHIAKKKTPLYEDYLT
                     SPAEEENYASADQNLSESLWDEPDMPDAVTQNRETARVLKEAIQSLAPQYRTIVTLFH
                     MDDMSYAEIGEIMQMPEGTVKSYLFRARKMLKARLLENFSTEELI"
     gene            6685..7020
                     /locus_tag="PK754_07185"
     CDS             6685..7020
                     /locus_tag="PK754_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09324.1"
                     /translation="MQNLEFIIPIAFFWMVAMIVKIVTDNRTRQKLIDKGLVDEKVKY
                     LYARDLRENPLQSLKWGIVLVGIGIALAIGQIFPMMEGPALMGLMFIFAGIAFMIYYV
                     LAKKQTPNE"
     gene            7118..8374
                     /locus_tag="PK754_07190"
     CDS             7118..8374
                     /locus_tag="PK754_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09325.1"
                     /translation="MRSAILTLFCVWAVIKPTILVGQNNFHIGFVQNNNSRLNHIDAY
                     YNDMLHSLEVKAIGVVELSDDDRDFKFLAENATVQIKTRNWFTYREVRVRGLRDGQLE
                     RTFYANGREMDWNEYGQSWLAAELPEIARKTGLGAAGRVQRLIAQRGVDAAINEIEFQ
                     ENNYARQIFCESIMAHENLTSAQLQRTVIVTAKEISSSSRLGDLLTDATTKFPQDSTF
                     TLTLMRAVREISSSSRQSETAITIARRRGLDRESAIEMARTIEDVSSSSAQKNALETL
                     AELAPPHEEAFLAYLHAVRTVSSSSAQSEALLALTQRTLTRDAWIQLFKVTESVSSSS
                     AQARVLSRSAEVCPNDDMIWFTFLETVGTVSSSSAQETALSAMLRRQGLSKSVLKRTL
                     QSVENSVSSSSVQRYIEGLVHDRLME"
     gene            8513..>10698
                     /locus_tag="PK754_07195"
     CDS             8513..>10698
                     /locus_tag="PK754_07195"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HNB09326.1"
                     /translation="MYRLIFLLLLSSLSYAQKNITGAITDKSNGEPLPSANIQIEGTL
                     RGVVASVEGRYALRIESLPATIVVRYIGYESQKLVITENSPSEQNISLTPSSVQMEEM
                     IVIGEDPGIRIMRNVIAKKKRWHKLINNFKADGYNRMVMENDSGIVMISESLADTYWD
                     RDKGLKVVIKSKRMTKNVRPQDNIFVGEEGMVNFYDDDIEIQGSRFVGPTHPDALDYY
                     DFKITGRRYLDDYLVYDIAVRSKTKLQPTFIGSISVIEEDSAMVAVDLKPTEHVIFPM
                     PIQQWNVNYKQQFSNFGQAYWLPVDIRMHGNIKVAMPGLEFPKIIYSSLMGVNNYQIN
                     IELPDSLYKSRKSFFVDSVALAKPNQFDSSAIIIPRTEAEDSAFTNIKRGDSFTKAFK
                     PKGALARYVKMEDDYNDSVDTEKKKKTAFQKFLSDLSPVVGFDRVEGGRLGVKYEHEF
                     SKHWSAYIKGGYTTTLEKAFYGAGGEHRFGYRRRGHLEAEYYNGVDMRQNSENYNLLM
                     TSGQPLFGYRDYFDYYKSHRFKVTGGYNFAFQDLDFSAGFHQDIQKSVSKTTDWDIIG
                     RKYDQRKNPAIDKGTLRSFIFTGTWGEKYVPMGVVGNNGLTVSIEHSDKKILSSDFGF
                     TTYKVSGNVRIKTFLRRRFIPNTLDVYATAGTYTGKLPVQRFGVIDGSLGYFTPFGTF
                     KTLRTRGYEGEKYAAVFAEHNFRSLPFELIGLDWFARKNIGIAVFGS"
BASE COUNT         2922 a         2611 c         2313 g         2852 t
ORIGIN      
        1 tgtcatcgct tgacgaaaca ccgtccagta agcatctttt ttcggaaatc ctttatctaa
       61 acgtttccac gtttttcccg cattgtacgt tgcatagact gccggtttac cgccgggtga
      121 agtacgcggc caaaccgacg tcccgtccat cggaaaaacc caggccacgt ccggatccgt
      181 aggatgcagc gtaatcgaaa aaccgatatc gccgatatgt ttgggcaggt ttttcccaat
      241 tcgataccag cgaccctccg tgcgatccat acgatacacc ccgcaatgat tctgctgata
      301 caagcggtcc gggatgagcg gatgaagttg tacgcagtgc ggatcgtgac cgtactccgg
      361 atcaggctgc ggtaaaaatt ccgcatcaca acctttattc aaaggtgtcc acgttttgcc
      421 ttgatctgcg gtttcaaaaa caccaccgct ggagcaacca agatacatat gacgtgcatt
      481 acgcggatct acaataatcg aatgaagttt ggggccgtcc ggggtgccgt ctttatcgcc
      541 accagtccat ttttcataca tcggattttc attgaagccg tctacatttt tccacgtcac
      601 accatgatct tcgctgcgaa acaaaccctg aggcgatgta ccggcatacc acactccggg
      661 ttcactggca tgacctggcg tcaaccaaaa gacatggtct accgcacgtg gacgtttggc
      721 cgaatctgtt ttatgaaacg ccggcggttg tttggattct ttccaggttt taccgcgatc
      781 atcagagtga aataccgtag gcccgagatg acctgttttg caagccatca gccaacattt
      841 ggattcgcgc ggatcctgca tcagatgatg tacgatatgc cccaaaaaaa tcggtgccga
      901 tactttccac gtgcggcgcc gcgcatcgct tttgagcgta aacgcaccct tacgcgtacc
      961 gataagcaat gttacgggcg aattttttga acgtgaagat ttcatacaac ctccttgcac
     1021 gacgtgcttt acgttctcta aaatatatag ctaaaactat gcttcgttta taccggactt
     1081 gcgacgggca cgaatgaatt cgatcacggc aggaatcacc gatatgatga tgatcgccac
     1141 aatcacgagg gtaaagtttt ttcgcacggt cgggatatta ccgaaaaaat aaccgctaga
     1201 aacgaacagt ccgacccacg cgatagaacc gatgatgttg tagaaaataa agtgacggta
     1261 cgtcatttga ccaataccgg caacaaaagg agcaaacgtg cgaatgatgg gaataaagcg
     1321 cgcaaaaata atcgttttac cgccgtgttt ttcgtaaaac atatgcgctt gttgtaaata
     1381 ttctttattg agaaaacgta cattttcttt atgaaaaatt ttcggcccga tgcgtaaacc
     1441 cacggcataa ttgaccgaat caccgataat accggctacc gtgagtaaaa taaataacca
     1501 ccacggatca agataaccta agccggcaaa cgcacccgcc gcaaaaagaa gcgaatcacc
     1561 cggtaaaatc ggtgtgacca ccaatccggt ctctgcaaaa atgatgagaa ataaaatcag
     1621 atatgtccat gtaccgtaat tctgaatgag ttcgcctaag tggacatcca gatgaataaa
     1681 aaaatctata aactgcgcaa tcaattccaa tgtatcgatc ctcgtttgtt agtttgaaag
     1741 ggctaccgta ggtgctggaa tgcggcctcc gcgcccgata aattttttag gtgataaatt
     1801 acgaatcggc atgatcggcg ctttgcccag cagtccgcca taatcaacgg attcgccgac
     1861 tttttttccg ggtacgggaa tgatgcgaac cgccgtagtt ttgctgttga tcattccgat
     1921 agctgattcg tccgcaatca gaccggcgat tgtttcccac gttgtatcac cgggcacggc
     1981 aaccatatcc aaaccgaccg aacacacaca ggtcattgct tctaattttt ccaacgacaa
     2041 atttcctttt tctacggcag cgatcatacc ttgatcttcg ctcaccggga taaaagcgcc
     2101 gctcaaacca ccgacatacg acgacgccat gagaccgcct tttttaacgg catcgttgag
     2161 catcgcaagt gccgctgtcg taccgcatgc gccgacatct tcaagtccca tcgcaacaag
     2221 aatatccgcg atactgtcgc ccggcgccgg tgtaggtgcc aaagacaaat caacaatacc
     2281 gaaatcaata ttctgctgac gcgccacttc acggccgatc aattcgcccg cacgggtaat
     2341 tttaaacgtc atacgtttga taatttccga gagttcgcct aaatccgcat ccggcgcttg
     2401 tttgatcgca tgcagcacga cacccggtcc gctgattccg acattgatcg tgcattcggc
     2461 ttccgtcact ccgtgaaatg ccccggctac aaaaggatta tcttcgaccg cattacaaaa
     2521 cacgacaaac ttagcacaac cgatcgcgcc ctcggacgcc gtgacctgcg ccaaatcttt
     2581 gatgactttg gataccgcaa taatggcatc catattgata ccggctttgg tagaaccgac
     2641 attgatggac gaacatacgc gtttggtact acctatggct tcgggaatgg attcgatcaa
     2701 tgcccgatca cctttggtaa aacccttttg tacgagcgct gaaaatccgg ccaaaaaatc
     2761 tacaccgacc cgttcggccg cttcatctaa ttttttcgcc aactgaacaa attcactacg
     2821 cgtaaatccg tcacaagcaa tggatgccgg tgtgatggaa atacgtttat tggcaatggc
     2881 tatcccgtat ttttgttcga cctcacgcgc cgtcgctaca tgacgttccg cgtaacgaca
     2941 gattttatcg tagatcgcct ggatcgtcgc cgacgcatta ccgcgtccgc aatctcgtaa
     3001 actgataccc atcgtcgtcg tgcgaatatc aaaatgctct acctccgtca tgcggatggt
     3061 ttctaaaatt tcttgaattt caaatgacat atgacctcaa tataagaaca ttagaaaact
     3121 attttttctt tattacacgg atcaactgag aattcatgcg aattgtcatg acgagatcaa
     3181 acccggaggc gttttcgctc atgctttgcg gttcaagagc ttacacatgg gatatattca
     3241 atttcgtcca tatttccttt tggagcgccg gtccgcacgc aatcgcgttg tcaatataca
     3301 tagacgtaac gccatttcga tcaaaaatca cagcgccgga ttcttcgatg atcggcttga
     3361 gtgcggcgat atcccaaaga ttgagcttag ggtccaccat aatatcggcc cgaccgcacg
     3421 ccaccaaagc atgcccataa caatcgcccc acgtgcgaac gattttagtt tctttgatga
     3481 gtgtttgaag aatgggtgcg tagtctcctt ggtgagaact tttcatatcc gtggtgagaa
     3541 gcgtcgcttc agccagatat tccgttgtgc gcgctttgac tctacgaccg ttgatagttg
     3601 caccttcatt agtagccgcg gaaaggattt cgcccagtgc cggaaaatgt atcacaccga
     3661 cgatggcatc ttttttttgc acatcttcga tagcaaccat cgtcccgtaa aaaggtacac
     3721 cgtggataaa tgatttggtg ccgtcaatag gatccaatat ccagcgatac cggtcgctac
     3781 cgatagtttc gccgaattct tctcccaaaa ttccatgacc cggaaattct ttactgatga
     3841 atgcgcgcaa ccattcctcc gcagaacggt cggcgatcgt gacgggcgag gcgtccggct
     3901 tggcgatcac atccaaggag gccgtctgat aataacgcag tgtgagtttt cctgcttcat
     3961 acgcggcatg tgtagccgcc tgaagcaact cacggatttc ggaagacatg gagaacaacg
     4021 ctgaaaaatg aacgatttta cgcgttgatt ttactgaaaa acgaaatgat aatcaaacgt
     4081 attcacgtgc cggcttcggc cagcgttttt attttttcta aaatatacga ccaacccccg
     4141 tgggaatcct gataacgctt ttcaccgtta gcgaccgtcg aaaaatcccc ttgtacgacg
     4201 gacacgtcgg ttgcggattc cttttcacta agtgtgatgc tcaccgtcag ataatttgca
     4261 gggatatccg catatcctcc aaatgggtcg aaaaccgtgt actccaaaat acggggatat
     4321 tcattacgaa cgatatgacc gcgcacatat acgacgccgt cctttgcgct tttccattct
     4381 aaacgactgc ctggtttcca atcgctttgc gcttcacagt caaacatgta ctgccgcgtc
     4441 aacgacggtt cagtcaacac cgcccacacc cgctcggctt gggcatatat cgttatttct
     4501 gaagtaattg tcttagccga cataaattga tccttcaatt tctttgtttc aattttcgtt
     4561 cgttgccaat atactcataa gaaactcatc ataattgtaa aaacacgaca aatgacgaag
     4621 actaatagcg ccggtaaacc gcgaggcatt ctgagttcta ttccaaatcc ccgtattcgt
     4681 tattaccgcg acaaacctac gaaaacgctc caatctacga tcgaacattt ttggatggta
     4741 cgatgggatt ttccgaaaaa ccgcccgtat atagctgaaa ctctgccgca tccttctgtg
     4801 catttggttt tcgagaaaga cggctcctct attggcggac cggttaccgg aaaatttaaa
     4861 cgaactctga atggtaaagg ttttgttttt ggtgttaaat ttcttcccgg cgctttttcg
     4921 gcattttata aaccgtctat agttaacctg gccaatcaca aagtaaacat cgaatccgtt
     4981 ttcggtgaat ccattctgca attagagcgt tccataatgc gcatgaagcg atttgatgca
     5041 atgaaaaaag ccgctgagtc attttttaca tccataccga tcaaacatga cccgcgtgct
     5101 atccttgcac aaaccatcgt agcaacggtc atgattgaac gtgatatcgt tcacgtgcgc
     5161 cagcttttag cgcggttcca tattaccgcc cgttctcttg aaagattgtt tgaaaataat
     5221 atcggcgtaa cgccaaagtg ggtgattcaa cgttaccgct tacacgaggc tgtagaaatg
     5281 ataaaatcta acgcagaaat ttcattgacc aaactggcct tggatctggg atatcatgat
     5341 caggcacatt tcattcgtga ttttaaatcg ctaatcggca tgacaccgtc acgtttttta
     5401 cgaaatgcga cgggcaaaac tgtttagtgc tgcaccctca atctcaaaaa tttccgctcg
     5461 atcatatcaa aaagcaatac gagaaataac ccaaaaataa gatactgaaa attggaaccg
     5521 atccaaccgc ccatttgttc agctatcgta aaataggttt gcgtctgccg aatattttcc
     5581 tgtaaaacgc gctcaaccgg tgctccggtc atcgtagaag gcaaataata cgcggaaatg
     5641 ccgatgcccg ctgcgacgac cggcaacagc caccaccact cacgtaaaac agatgtttcc
     5701 gactcatcag cggaaaccaa acccactttt tccgccacta tatccgcaaa atgtgccggt
     5761 aatataaatt cttcctgacg tatggtgtgg tgcatcatcc gatatatggc catagactcg
     5821 cggcattcct cgcataccga cagatgcgtt tccatgtttt gtttggttat accggcggtc
     5881 ccgtccagat acgcctgcat catcgcatca ctgagatgat tcatattaat tcctccgtac
     5941 taaaattttc aagtaaccgc gctttgagca ttttgcgcgc ccggaaaagg taacttttta
     6001 ccgtaccctc cggcatctgc atgatttcac cgatttccgc atacgacatg tcatccatat
     6061 gaaaaagcgt tacgatggtc cgatactgcg gcgctaatga ttggatcgct tcttttaaaa
     6121 ctctcgctgt ttcccggttt tgtgttacgg catccggcat gtcgggttcg tcccacaggc
     6181 tttccgaaag attctgatcg gccgatgcgt aattttcttc ctccgccggt gaggttagat
     6241 aatcttcata cagcggcgtt tttttcttcg cgatgtgatt tagcgtcgta ttataagcga
     6301 tgcgggcaat ccatgtcgaa agtttagcat taaactgaaa ggtccggaga ttttcataca
     6361 ctttggtaaa aacttcctga caaatctcct gttgatcgga tggattggat accattttaa
     6421 atacgatatg cgcgaccaat ttttggtggt cttcgactaa ctgcttaaat gccttagcat
     6481 cgccggcaat tattttttct atagttaaac gctcattttc catagatggt cttatcatac
     6541 agggtattag accgacgaag cgaacaaatg ttgcaataaa ataattagct aacaagtgca
     6601 accttttgct ttttgaccgg tcttatcggg tagaacttat gaaatcaaaa aaacgtatta
     6661 caccatattg aaaggaacca gattatgcaa aacctcgaat tcatcattcc tatcgcattt
     6721 ttttggatgg ttgccatgat cgtaaaaatt gtcacggaca atcgtacgcg ccaaaaactg
     6781 atagacaaag gattagtcga tgaaaaggta aaatatcttt atgcccgtga tctccgcgaa
     6841 aatccgctgc aatcgctcaa atggggcatc gtccttgtcg gtatcggtat tgcattggcc
     6901 atcggccaga ttttcccgat gatggaaggc cccgcgctga tgggtctgat gtttattttc
     6961 gcgggtatcg ctttcatgat ctattatgtc cttgctaaaa aacaaacacc gaacgaatag
     7021 aatattgggt ttacctaaga actattcgct ttataaacga agcattttgt tggatcaatt
     7081 tatttcattc atacgatacg tttagaggaa aaaccttatg cgctccgcca tacttacttt
     7141 gttttgtgta tgggctgtaa taaaaccgac gatcttagtc ggacaaaaca atttccatat
     7201 cggttttgtt caaaacaata atagccgcct caaccatatt gatgcttatt acaatgacat
     7261 gctgcacagt cttgaggtca aagctatcgg tgtggtcgaa ttatccgatg atgaccgtga
     7321 tttcaaattt ctagcagaaa atgctacggt acagatcaaa acccgcaatt ggtttacgta
     7381 tcgcgaagtc cgggttcgcg ggttacgtga cggtcagttg gaaagaactt tttatgctaa
     7441 tggccgcgag atggattgga atgaatacgg ccaatcatgg ctcgctgccg agttgcctga
     7501 aattgcacga aaaacaggac tcggcgctgc gggtcgggtc cagcggctta tcgctcagcg
     7561 cggcgttgat gcggcaatca atgaaatcga atttcaggaa aataattacg cgcggcagat
     7621 tttttgtgag tccattatgg cacatgaaaa tttgacctct gctcagcttc agcgcaccgt
     7681 tattgtgacg gccaaagaaa tctcaagcag ttcgcgcctc ggtgatttat taacggacgc
     7741 cacgactaaa tttccgcaag attcaacctt tacgttaacg ctgatgcgtg ccgtacgcga
     7801 aatatcctcc agttctcgtc aaagtgaaac ggctatcacc atagcgcgcc gtcgcggctt
     7861 agatcgtgaa tcggctatcg agatggcccg cacgatcgaa gatgtcagct ccagttcggc
     7921 gcaaaaaaac gcattggaaa cgctcgccga attagcgcct ccgcatgaag aagcgttttt
     7981 ggcatacctg catgccgtac gcacggtaag ttcatccagc gcacaatccg aagcgttact
     8041 cgcgcttaca caacgcacac ttactcgcga cgcgtggatt cagcttttta aagtaaccga
     8101 atcggtcagt tcgtccagcg cacaagcgcg tgtgctaagc cgtagcgcag aagtctgccc
     8161 caatgatgat atgatttggt ttacttttct tgaaaccgtc ggaaccgtaa gctcaagttc
     8221 cgcgcaggaa accgctcttt ccgccatgct gcgtcgtcag ggcttgagca aatccgttct
     8281 caaacgcact ttacaatcgg ttgaaaattc ggtgtcgagt tcttccgtac aacgttatat
     8341 cgaaggtttg gtacacgatc gcttaatgga ataagcgtcg attcctcaaa gtataagaga
     8401 agaatctatc tatgagttct tttaatttat aaaactttgt attggaactt cacgccgact
     8461 tacacgtaca tggggtcggc gtttgtatta catattatga ggggtttatc acatgtatag
     8521 actgattttt cttctgctgc tgagttcact ttcctacgct caaaaaaaca tcaccggggc
     8581 gatcactgac aaaagtaacg gtgaaccttt accatcggcc aatatacaaa tcgaaggcac
     8641 gctgcgcggg gttgtcgcca gtgtcgaagg acgatacgca ttgcggatcg aatccctacc
     8701 cgctacgatc gtcgtacgtt atatcggtta tgagtctcaa aaattagtca ttacggaaaa
     8761 cagcccttcc gaacaaaaca tcagcctgac accctcctcg gttcagatgg aggaaatgat
     8821 cgtcatcggg gaagatcccg gcattcgtat catgcgcaat gtcatcgcca agaaaaaacg
     8881 ctggcataaa ctgatcaata attttaaagc cgacggctat aataggatgg tcatggaaaa
     8941 cgattcaggt atcgtgatga tttccgaatc tttagccgat acctactggg acagagacaa
     9001 aggactcaaa gttgttatca aatccaaacg tatgaccaaa aacgtccgac cgcaggataa
     9061 tattttcgtg ggcgaagaag gcatggtcaa tttttatgac gacgatatcg aaatccaggg
     9121 atcgcgtttt gtcggaccta cgcaccccga tgcattggac tactatgatt tcaaaatcac
     9181 cggacgtcgc tatctggacg attatcttgt ttacgatatt gccgtacgtt ccaaaactaa
     9241 attacaacct accttcatcg gcagtatttc cgttatcgaa gaggactctg cgatggtagc
     9301 cgtagatctc aaaccgacgg aacatgtgat ctttccgatg cccattcagc aatggaatgt
     9361 gaactataaa cagcagttct ccaatttcgg tcaagcctac tggttaccgg tggatattcg
     9421 tatgcacggc aatattaaag tcgcaatgcc cggtttggaa tttccaaaaa tcatatacag
     9481 cagtctgatg ggcgttaata attatcagat caatatcgaa ttaccggatt cactctacaa
     9541 atcgcgtaaa tctttttttg tagattccgt cgcgttggca aaacctaatc aatttgattc
     9601 ctcggctatt attattcccc gtaccgaagc ggaagattca gcattcacca atatcaaacg
     9661 cggggactcc tttaccaaag cattcaaacc caaaggggcg ttggcgcgat acgtcaaaat
     9721 ggaagatgat tacaatgaca gcgtggatac ggagaaaaag aaaaaaacag cgttccaaaa
     9781 attccttagc gatctttcac ccgttgtcgg ttttgatcgt gtcgaaggcg gacggcttgg
     9841 cgtgaaatac gaacatgaat tttccaaaca ttggtcggca tacatcaaag gcggctatac
     9901 gacaacattg gaaaaagcgt tctatggcgc cggtggtgaa caccgctttg gttatcgccg
     9961 ccgcggtcac ctcgaagccg aatattataa cggtgtagat atgcgccaaa actcggaaaa
    10021 ctataatctc ctgatgacca gcgggcaacc gctcttcgga tatcgcgatt attttgatta
    10081 ctacaagtca caccgcttca aagtcaccgg tggttacaat tttgcttttc aggatttgga
    10141 tttttctgca ggatttcatc aggatatcca aaaatccgtt tccaaaacca cggactggga
    10201 catcatagga cggaaatacg atcaacggaa aaaccctgcc attgataaag gtacactgcg
    10261 cagttttatt tttacaggta cctggggaga aaaatatgtg cccatgggcg tagtcgggaa
    10321 caacgggctt acggtctcca tcgaacattc ggataaaaaa atactctcca gcgatttcgg
    10381 ttttactaca tataaagttt ccggaaacgt gcgcattaaa acatttctcc gtcgccgttt
    10441 catccccaat acgctggatg tatatgctac cgcaggtacc tacaccggca aacttcccgt
    10501 tcaacgtttt ggtgtgattg acgggtcgct cggatatttt accccatttg gtacgttcaa
    10561 aacattacgt acacgcggat atgaaggtga aaaatacgct gctgtttttg cggaacataa
    10621 ttttcgctcg ctaccgtttg aattgatagg attggattgg tttgctcgta aaaatatagg
    10681 gatcgccgtt ttcggatc
//
LOCUS       DAOQNQ010000088        10696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2214_length_10696_cov_5.400902, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000088 DAOQNQ010000000
VERSION     DAOQNQ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10696
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2214_length_10696_cov_5.400902"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PK754_07200"
     CDS             <1..277
                     /locus_tag="PK754_07200"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006656209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNB09327.1"
                     /translation="IAVFGISPDSVKKHQNFTKKHELPFPLLADTEHRVIKMYGVWGP
                     KKLFGRAYQGVLRTTYIINEKGNIAFVFDKVHAETHAEDVLKAIKAL"
     gene            274..777
                     /locus_tag="PK754_07205"
     CDS             274..777
                     /locus_tag="PK754_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09328.1"
                     /translation="MKKTIYTATILIFCCVAPLATAQTSWETHRFEPYRIEFKTPPHW
                     QISINTDGEKSFIECYSPDSEIYFFITVAENEKKSDPDIVLQYLKVGSLNSEFIRDEA
                     RKINNIDFKFSIGIKTVNNYSTYIRIGVGQYKDKIYMLESGFSRVDSDASEALLDQVI
                     SSFKAIR"
     gene            784..2019
                     /locus_tag="PK754_07210"
     CDS             784..2019
                     /locus_tag="PK754_07210"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB09329.1"
                     /translation="MKKQLLILGTGFGGFSLMKGIRSDLYDVTVVSTRNHFLFTPLLP
                     STTVGTIEFRSIIEPVRLARKDVTFYQAGATALHPEKRTVTCRNIADAQTFELHYDTL
                     VIAVGAVNNTFKIPGVAEYAYFLKELRDARRIRQRIIDNFERASTPGLDAAEMQRRLH
                     IVVVGGGPTGVEFAAEMADFLEEELEKGFPHLVGMAKITLIEAGKQILSAFDHTVSEY
                     AIKLFNRRRVTILTGKSVKEVTAQELTLHDGTQFSHGLVVWSTGNGPTDFVQSLSCPK
                     DRAGRILVDSFFRVKDNPDIYALGDCATTEEKSLIATAQVANQGGKYLANYLNQLAKG
                     KSAESLKPFVYKHLGMLAYIGDNRAVADFDATTSQGFATWIFWRSAYLTKLVSLKNKA
                     MVLFDWAKAFLFGRDVSRF"
     gene            complement(2030..2881)
                     /locus_tag="PK754_07215"
     CDS             complement(2030..2881)
                     /locus_tag="PK754_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NotI family restriction endonuclease"
                     /protein_id="HNB09330.1"
                     /translation="MANGNALSELYSYNTADDEVKWKNVIEKQYCPFISRKCLKNRKS
                     DAKQSIGSCSIQYGKESDTIIVCPFRFLERNKIFFDSIHLLRLHEPGNELHVINEFSI
                     PGGNVDYILASARDGKVKDFVGIEMQALDTTGTVWPSRQKLLHKFGVKVLKSDRESTD
                     TFGVNWKMTAKTILVQLHHKILTFESLHKHLLLVVQDKMMDYMTSQFDFSSLHDTLNG
                     DSMHFHMYEMSKKGHKFQLVLKNRKSTDADGIAKCLGLQANPQVELNIILETLTKSIS
                     EKTILKF"
     gene            complement(2860..3939)
                     /locus_tag="PK754_07220"
     CDS             complement(2860..3939)
                     /locus_tag="PK754_07220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNB09331.1"
                     /translation="MIYKYPLNFQQVVNDLEKDSHNEPLFISGEALLVLKSFPDSVID
                     MAMTSPPYWGKRDYQNDGIGLEADPIQFIDNLLNVFNELKRVLKPTGSFWLNIGDTYR
                     NKSLLGLPWRIAIRMIDEQGWIMRNSVIWNKLKGSMDNSPDRLANVHENLFHFVKSKH
                     DYFYDTNAIRKKPKNVIIKNGSIISATGVSGVRYRRQIELSSELSEAEKKNAIEDLDV
                     MMNQIRLGELSDFRMVIRGHQRATHSDAESISGRARELKQKGYYFLKYHPNGSKPADV
                     WDIIPEDSQKRKIHYAPYPEDLCRIPIAATCPPNGIVLDPFCGTGTTMAVAKKMNRKS
                     IGVDLSSDYIQYANKRCEVWQMEMH"
     gene            complement(3975..6830)
                     /locus_tag="PK754_07225"
     CDS             complement(3975..6830)
                     /locus_tag="PK754_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HNB09332.1"
                     /translation="MKSLLSAIRLQHFYGIALHRTGGDIWEDAITHVAAAQFIDGKIT
                     VVLDEACASDDTREAVLRRLQDTIGDVPLVLFSSADIEFLHASFHAMYDNKDRASPFL
                     MNECWDLQRLASTLLPVISFATLEELAVYFTFPEGQNPAPSEAVILIFYRLIESLARM
                     EPRIVSQANRFLEHSKSPLKRILRGLADYIRDGKNIHFIFPEVQWPTPAPNVIGVADG
                     GFFSDTIHSVPDSDIRRFFSPQGGLSRSMETYEERAEQTRLTVAINSAFNASQHILAE
                     AGTGTGKSLAYLIPAIHWASYNNALGEQVVISTHTKNLQDQLFYKDIPLLARTLPFGF
                     RAVLLKGRSNYICMKKWTHITADAGSHVQPQEREKLLPILFWIHKTRTGDISECVGFQ
                     AEANPGVWHKLASESAYCQAQRCSRSEDCFVRKIREEAKQAHVVVVNHALLFSDLVSD
                     NAILGDYHNLVLDEAHNVEKVAQNYLGAEFSLWAVRGLTSKLYERDQTETGVLIQLRQ
                     KLQKSRLSDVLQSAFLSQIQTGTDACVQFWLAAQTLFQRLTTEALRKTEFAMQSDKAV
                     RGERRVRYGPTDSLLLRVADDVDLMHEKATKLRQALDVLSETMRQWPTDAMDGAEEMR
                     DLIILRTDELDALLKTVDFLTEGANKDYVYWYEMPPKERSYDMRFYAVPLNIADILRN
                     TLLEKLKTCVFSSATLSVAGQFNYYKRRIGLDAMRSEKVQEFTADSPFDYKNQSRILV
                     PTFLPEPQSPKYNDACAELLKRITLETRRGMLVLFTSYQMMNSCYYQLRDPLNAAGIP
                     LLMQGRDASRTQLTEHFRRERVSVLFGTDSFWEGVDVPGSALEILVITKLPFEVPSDP
                     LVAAKMERIDAQGGKSFFDYSVPEAVIKFRQGFGRLIRSRSDRGVALVFDNRVVTKSY
                     GSYFMRSLPVQALRMPTVDGLIDNIDNFLGFEFLNQK"
     gene            6942..7214
                     /locus_tag="PK754_07230"
     CDS             6942..7214
                     /locus_tag="PK754_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09333.1"
                     /translation="MRNETAMTDEFKTQCVTLAREIQEALTTLEVATDPSSEERERIE
                     AKNVVANVLRSYKSLIENLSGTDKTEASKLLHKKIEDMALKAKHLR"
     gene            complement(7226..8119)
                     /locus_tag="PK754_07235"
     CDS             complement(7226..8119)
                     /locus_tag="PK754_07235"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB09334.1"
                     /translation="MAKKLLITGGTGTLGSHLVRQAIASGAWDEVHATNYSQRPNFHK
                     VFWYPMDARQPVGAIIERIAPTHILHTLALSSPDICEKNKLEAWQVNVSVTQSLAEYA
                     AQNKARLIYTSTDLVFDGTQGNYAENDTPQPLNFYADSKLEGEQAVREVMKDGHFVIV
                     RLANLYGPNLNGKENFFTQMTKALRQSSELKLFTDQIRSVMSASNAAACLLELADLSF
                     EGLLHLGGPQAVSRYEFGQILAHAMQVKNPSIKKITMAQMPGGAKRPANVSLKITQAQ
                     NILTTKLLAPEEGLKREIEGY"
     gene            complement(8126..8968)
                     /locus_tag="PK754_07240"
     CDS             complement(8126..8968)
                     /locus_tag="PK754_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09335.1"
                     /translation="MGDAKLHQLDFHLHHLPRLNRTEAQATLVPTDAVESVQAIFLSV
                     YRTYKSQSRRWISVTRIEVSFYNYTQFKHTMQIRDHTARVRISASARTEPAQFFEALA
                     HVLWSKIFGVRCPDAIMQYYKDAEWRLSREKETETKPTIKRFAPPKGRVYDLAQIKDQ
                     LDAVYFDRSMATVQIGWSLQKARTRLGHYDRSAQMIIMSPVLDHPDVPPAVVTAIVYH
                     EMLHHKHPVVYDEGRQIIHSRAFQQEEKRFVHYAEAQHWLKTEYPQFLTRRRGPRWFK
                     SWFT"
     gene            9303..9539
                     /locus_tag="PK754_07245"
     CDS             9303..9539
                     /locus_tag="PK754_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09336.1"
                     /translation="MHTMEIMSENIQGDEMASKGRVALRALGFMILALIIASIAVIFN
                     APSILMGITGILFPIGLFIMLLVAFYLLILSTKG"
     gene            9720..10673
                     /locus_tag="PK754_07250"
     CDS             9720..10673
                     /locus_tag="PK754_07250"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNB09337.1"
                     /translation="MPVSSSKTLRRLDYFFILRPILFIPGWTTTLAGYMAATGQAKHW
                     PVWNGLWYPDMMGLFISTAMLMGSGFLVNQLRDIETDRINKKLFFLYDGSVNPRILIL
                     QSILLSAGCLVMASFWGERMLIVHFFGWLLIAILYNLPPFRLKDRAVTGLLANAGMGV
                     FALAYGWALSDQSWLLFLITGFPYFIFNGALCVLTMIPDTEGDAATSKKTFTVRYGVT
                     FSMYTAFVLFCIAAITAFILDQYVLLVVLVSVIPMFVFMVIRKTKADAIRALKFSIFF
                     FTSLVAMYFPFYIILLIFLFVCTRWYFKTRFDFDYPNLQGR"
BASE COUNT         2830 a         2548 c         2304 g         3014 t
ORIGIN      
        1 gatcgctgtt ttcggtatca gcccggattc ggtaaaaaag catcagaact ttactaaaaa
       61 acacgaattg ccgttcccgc ttttggccga tacggagcac cgcgtgatca aaatgtacgg
      121 ggtgtgggga ccaaaaaaat tatttggtcg tgcgtatcaa ggggtcttac gcacaactta
      181 tattatcaac gaaaaaggaa acatcgcctt tgtgtttgat aaagttcatg ccgaaacgca
      241 tgcggaggat gttttgaaag cgataaaagc cttgtgaaaa aaacaatcta cacggcgact
      301 attcttattt tttgctgcgt cgcgcctttg gctaccgcgc aaacttcgtg ggaaacgcat
      361 cggtttgaac cgtatcgtat cgaatttaaa acgccgccgc actggcagat cagtatcaat
      421 acggacgggg aaaagagttt tatcgaatgc tacagcccgg acagcgagat ttattttttt
      481 attacggtcg ctgaaaacga aaaaaaatcc gatcccgata ttgttctgca atacctcaaa
      541 gtcggctcgc tcaattcaga gtttatccgc gacgaggccc gcaaaatcaa taatattgat
      601 ttcaaatttt cgatcggcat caaaacggta aataactatt ccacgtatat ccgtatcggc
      661 gtcggtcaat acaaagacaa aatctatatg ctagaaagcg gttttagccg tgtggacagc
      721 gacgcctcgg aagccttact cgatcaagtc atttccagtt tcaaagcgat tcggtaacct
      781 atcatgaaaa agcaattgct tattctaggg acgggattcg gcggattcag tcttatgaaa
      841 ggcattcgtt cggatctcta cgatgtcacc gtggtaagta cccgtaatca ttttttattt
      901 acgccgctgc tgccgagcac taccgtcggc acgatcgaat ttcgcagcat catcgaaccc
      961 gtacggcttg cacgtaaaga tgttactttt tatcaggccg gcgccacagc gctccatccc
     1021 gaaaagcgca ccgtcacttg ccggaatatc gccgatgcac aaacttttga actgcattac
     1081 gatactttag tcatcgcggt cggagccgta aataatacgt ttaaaattcc cggcgtagcg
     1141 gaatacgctt attttctcaa agaacttcgc gatgcccggc gcattcgcca gcgtatcatt
     1201 gataatttcg aacgcgcatc cacacccggt ttggacgcgg ccgaaatgca acgacggctg
     1261 cacatcgtcg tcgttggcgg tggcccgacc ggcgtcgaat tcgccgcaga aatggccgat
     1321 tttttggaag aagaattaga aaagggattt ccacatcttg tcggcatggc taaaatcact
     1381 cttatcgaag ccggtaaaca aatcctgagc gcttttgatc ataccgtctc ggaatacgcc
     1441 atcaagcttt tcaatcgtcg tcgtgttacc attcttacag gcaagtccgt caaagaagtg
     1501 acggctcaag aactcacatt gcacgacggc acccaatttt cgcatgggtt ggtggtgtgg
     1561 tccaccggca acgggccaac cgatttcgta caaagtttat catgccccaa agaccgtgcg
     1621 ggacgcattt tggtggattc tttttttagg gtcaaagata accccgatat ctatgcgctc
     1681 ggcgattgcg ctacgacgga ggaaaagtct ttgatcgcga cggcacaggt agctaatcaa
     1741 ggcggaaaat atttggcaaa ctacctcaat cagcttgcca aaggaaaatc ggcggaatca
     1801 ttaaaaccgt ttgtttataa acaccttggc atgctggcgt atatcggaga taatcgggct
     1861 gttgccgatt ttgatgcaac cacctcgcaa ggttttgcaa catggatttt ctggagatcc
     1921 gcgtatctga ccaaactcgt cagtttaaaa aacaaagcca tggtgctgtt tgattgggct
     1981 aaggcgtttc tttttggccg tgatgtgagt cggttttaga agagtatttt taaaatttta
     2041 aaatggtctt ttcggaaata gatttagtga gagtttcaag aattatattt aattcaactt
     2101 gaggatttgc ctgtaacccc aaacatttcg caataccatc agcatcagta ctttttctgt
     2161 tttttaaaac caattgaaat ttgtgaccct ttttgctcat ttcgtacatg tgaaaatgca
     2221 ttgaatctcc gttcaaagta tcatgaagcg atgaaaagtc aaattggctt gtcatgtaat
     2281 ccatcatttt atcttgaact actaaaagca aatgcttgtg aagcgattca aaggttagga
     2341 ttttatggtg aagttgaact aagattgttt tagctgtcat cttccaattg actccaaacg
     2401 tatccgtgga ctcacggtca gatttcaaaa ctttaacgcc aaatttgtgc aataattttt
     2461 ggcgtgatgg ccacactgtt cctgttgtat ccaaagcttg catctcaatg ccgacaaaat
     2521 ctttaacttt tccatctcta gctgatgcta atatgtaatc aacattacct cccggaatgg
     2581 aaaactcatt aatcacatgc aattcattgc ccggttcgtg tagtctcagc aaatgaattg
     2641 aatcaaaaaa aattttattc cgctctagga agcgaaatgg acaaacaata atcgtatctg
     2701 attctttgcc gtattgaatt gagcatgagc caatactttg ttttgcatca gattttcgat
     2761 tttttaaaca ctttctactt ataaatgggc aatattgttt ttcgattacg tttttccatt
     2821 taacctcatc atcggccgta ttatatgaat atagttcgct tagtgcattt ccatttgcca
     2881 tacttcacac cttttgttcg catactgaat atagtcagaa gacaaatcca ccccgatact
     2941 tttcctattc attttttttg ctaccgccat tgtagtcccc gttccacaaa acggatctaa
     3001 aacaatacca ttaggcggac aagttgccgc aatcggtatt cggcacaaat cctcaggata
     3061 tggcgcataa tgaattttcc ttttctgaga atcttcaggt attatatccc aaacatctgc
     3121 tggcttactg ccatttggat gatattttag aaaataataa cctttttgtt tcaactctct
     3181 agcgcgacca gaaattgact ccgcatcaga gtgcgttgca cgttgatgac ctcttataac
     3241 catccgaaaa tccgacaatt ctccaagacg gatttggttc atcattacat cgagatcttc
     3301 aatcgcattt tttttctcag cttcagaaag ttcagatgac aactcaattt gccgtctata
     3361 tcggactcca ctaacaccgg tcgcagaaat tattgatccg tttttaataa taacgttttt
     3421 aggcttcttt ctaattgcat tggtatcgta gaaataatca tgtttagact ttacgaagtg
     3481 aaacaaattt tcatgtacat ttgccaatct atcgggtgaa ttatccattg atcctttcaa
     3541 tttattccaa attacgctat tcctcatgat ccacccctgt tcgtcaatca ttcttattgc
     3601 aatgcgccat ggtaaaccta atagcgattt atttctatat gtatcgccta tattaagcca
     3661 aaaagatcct gtgggtttca gaactcgttt tagttcatta aagacattca gaagattatc
     3721 aataaattga ataggatcag cttccaaacc aatcccgtcg ttttgataat ctctcttacc
     3781 ccaataagga ggacttgtca tcgccatatc aatgacacta tcaggaaatg attttaaaac
     3841 aagcaatgct tcgccagaaa taaataatgg ttcattatga gaatcttttt ctaagtcatt
     3901 aacgacctgt tgaaagttta atggatattt ataaatcatt ttcttctcat taaatagaac
     3961 ttaagtacag aatatcattt ttgatttaaa aactcaaatc ccaaaaaatt atcaatatta
     4021 tcaatgagtc cgtctaccgt aggcatccgc aatgcctgaa ccggcagtga acgcatgaaa
     4081 tacgatccgt aacttttagt caccacgcga ttatcaaaaa caagcgcaac gccgcgatcc
     4141 gagcggctgc gaatcaatcg gccgaacccc tggcgaaatt tgatcaccgc ttccggtacc
     4201 gaataatcaa aaaatgattt cccgccttgc gcgtcgatac gctccatctt ggccgccacc
     4261 agcggatcgg acggaacttc aaacggcagc ttggtaataa ccaaaatttc caaagcgctc
     4321 cccggcacat ccacgccttc ccaaaaacta tccgttccga aaagcaccga cacgcgttcc
     4381 cgtcgaaaat gctccgtcag ttgtgtacgc gacgcatcgc gtccctgcat gagaagcgga
     4441 atgcctgccg cattcaacgg atcacgcagt tgataataac aactgttcat catctgatac
     4501 gacgtgaaaa gcaccagcat accgcgccgc gtttcgagag taatgcgctt caggagttcg
     4561 gcacatgcgt cgttgtactt cggcgattgc ggttccggta aaaatgtcgg caccagaatg
     4621 cggctttgat ttttatagtc aaatggcgaa tcggcggtaa attcctgcac tttttctgag
     4681 cgcatcgcat ccaaaccgat gcggcgtttg taataattaa attgccccgc cacggacaat
     4741 gtggccgacg aaaatacaca ggtttttaat ttttctaaaa gtgtgtttcg taaaatatcc
     4801 gcaatattga gcggcacggc ataaaaccgc atatcgtacg aacgttcttt gggtggcatt
     4861 tcgtaccaat acacataatc tttgttggcg ccttccgtca gaaaatccac cgtcttgagc
     4921 agcgcatcca attcatccgt gcgcagtata ataagatcac gcatttcttc tgcaccgtcc
     4981 atcgcatccg tcggccactg acgcatcgtt tcactcaata catccaaagc ctgacgcagt
     5041 ttggttgctt tttcatgcat cagatccaca tcatcggcaa cccgcaacag caaactgtct
     5101 gtcgggccgt aacgcacgcg acgttctccg cgcacggctt tatcgctttg catggcaaat
     5161 tctgttttgc gcagcgcctc ggtggtcaac cgctgaaaca gtgtttgcgc cgcgagccaa
     5221 aactgtacgc acgcatccgt ccccgtctgt atctgtgata aaaatgccga ctgaagtaca
     5281 tccgacaaac ggcttttctg aagcttctgc cgaagctgaa tcaatacgcc ggtttccgtc
     5341 tgatcgcgct cgtacagctt ggaagtcaga ccgcgcaccg cccacagcga aaactcggcg
     5401 ccgagataat tttgtgccac tttttcgaca ttatgcgctt catcaagcac aaggttatgg
     5461 taatcaccca atatcgcgtt atcggaaaca agatcggaaa agagcaaagc atggttgacg
     5521 acgacgacat gcgcttgttt ggcttcttcg cggattttac gtacaaaaca atcttctgaa
     5581 cggctgcagc gctgcgcctg acaatatgcg ctttcggaag ccagtttgtg ccacacgccg
     5641 ggattggcct cggcctgaaa tcctacgcat tcgctgatgt ctcccgtccg ggttttatga
     5701 atccaaaaca aaatcggtaa taatttttca cgttcctgcg gctgtacatg actgccggca
     5761 tcggccgtga tgtgcgtcca ttttttcata cagatataat tgctccggcc tttgagcaac
     5821 acggcgcgga atccgaatgg cagcgtacga gccagaagcg gaatgtcttt gtaaaaaagt
     5881 tgatcctgaa gatttttggt gtgcgtactg ataacgactt gttcacccaa agcgttattg
     5941 tatgacgccc agtggatcgc cgggatcaga tacgccagcg atttgccggt accagtaccc
     6001 gcctctgcaa gaatatgttg cgacgcgttg aatgcggaat tgatcgcaac cgtgaggcgc
     6061 gtttgttcgg cgcgctcttc gtacgtttcc atactacggc tcagtccgcc ttgcggtgag
     6121 aaaaaacggc gtatgtcgct gtcgggaacc gaatggatcg tatcggaaaa aaagcccccg
     6181 tcggccaccc cgattacatt gggtgccggt gtcggccact gcacttcggg gaaaatgaaa
     6241 tgaatgtttt taccatcccg aatatagtcc gccaaaccgc gcagaatgcg tttcagcgga
     6301 cttttactgt gctccaaaaa gcgattggcc tgtgacacga tacgcggctc catacgcgca
     6361 agcgattcga tcaaccggta aaagatcaat atgacggctt cgctcggcgc ggggttttga
     6421 ccttcgggaa aagtaaagta caccgccaat tcttcgagcg tggcaaatga gatcaccggc
     6481 aaaagtgttg aagccagccg ctgcaaatcc cagcattcat tcatcagaaa aggcgaagca
     6541 cgatctttat tatcatacat cgcgtggaac gacgcgtgca aaaactctat gtccgccgac
     6601 gaaaataaaa ccaacggcac atccccgatc gtatcttgca aacggcgtaa caccgcttcg
     6661 cgcgtatcat cggaagcgca ggcttcgtcc aatacgacgg tgatttttcc atcgataaat
     6721 tgcgctgcgg cgacgtgcgt gatcgcatct tcccagatgt ccccccccgt gcgatgaagc
     6781 gcgatcccat aaaaatgttg gagccggatg gcggataata atgatttcaa aagcggtatt
     6841 tcgttttatg gtgcgccggc aaaatccgaa cggttttccg cgcaacaaaa ataattccga
     6901 attgacaatg cgcggcgctg tggctaaatt tatcaaaatt catgagaaat gaaacggcta
     6961 tgacggacga atttaaaaca caatgtgtaa cgctggcacg tgaaatccag gaagccctga
     7021 caacactgga agtggcgacc gatccgagtt cggaagaacg tgagcgcatc gaggcgaaaa
     7081 atgtagtcgc caatgtcctg cgcagctata aaagcttgat cgaaaatctg agcggcacgg
     7141 ataaaacaga agccagcaaa ctgctccaca aaaaaattga agatatggcg ttgaaagcca
     7201 aacatcttcg ctagtcgcag gtttatcaat acccttcgat ctcccgctta agcccttctt
     7261 ccggtgcgag cagcttcgtc gttaatatgt tttgcgcttg cgttattttc agcgacacat
     7321 tagccgggcg tttggcgccg ccgggcatct gtgccattgt tattttttta atgctgggat
     7381 ttttaacctg catcgcatgc gccagtattt gaccaaactc ataacggctc accgcctgcg
     7441 gaccgcctaa gtgcaaaagc ccttcaaacg ataaatccgc cagttccaac agacaagccg
     7501 ccgcattgga ggccgacatc accgaacgga tttgatcggt gaacaatttc aattccgatg
     7561 actgacgcaa tgccttggtc atttgtgtaa aaaaattttc tttgccgttg agattgggac
     7621 cgtacagatt ggctaaccgc acaatcacaa aatgaccgtc tttcatcact tcgcgaacgg
     7681 cctgttcgcc ctccagtttg ctgtccgcat aaaaattgag cggctgcggt gtatcgtttt
     7741 ccgcgtaatt cccttgcgtc ccgtcaaaca ccagatcggt cgaagtgtag atcagtcgtg
     7801 ctttgttttg cgcagcatat tccgccagac tttgcgtaac ggatacattg acctgccagg
     7861 cttccaattt atttttttca caaatatccg gcgatgacaa tgccagtgta tgaagaatgt
     7921 gggtcggcgc gatgcgctca atgatcgcgc cgacgggttg gcgagcatcc atcgggtacc
     7981 aaaaaacctt gtgaaaattg ggacgctggg aatagttggt cgcatggact tcatcccacg
     8041 cgccggaggc gatagcttga cgtaccaaat ggcttccgag cgtacccgta ccgccggtga
     8101 tcagtagttt tttggccata cgaatttacg taaaccagga tttgaaccaa cgcgggccgc
     8161 ggcgacgcgt cagaaattga ggatattccg ttttgagcca gtgctgtgct tctgcgtagt
     8221 gcacaaagcg tttttcttcc tgctgaaatg cgcgcgaatg aatgatctgc cgcccttcgt
     8281 catatacgac ggggtgcttg tgatgcagca tttcatgata tacgatcgcc gtgacgacag
     8341 ccggcggaac gtccggatga tccagtaccg gactcataat gatcatctgt gccgatcggt
     8401 catagtgccc taggcgtgtc cgtgcttttt gcaaactcca tccgatctgc accgtcgcca
     8461 tgctccgatc aaaatacacg gcatctaatt gatctttgat ctgcgcgaga tcgtacaccc
     8521 gtccttttgg cggggcaaaa cgtttgatcg taggcttagt ttcagtttcc ttttcccggc
     8581 tcagacgcca ttccgcatct ttgtaatact gcatgatcgc atccggacat cgcacaccga
     8641 aaattttgga ccacagcacg tgggccaatg cttcaaaaaa ttgcgccggt tccgtacgcg
     8701 cactggcgct gatacgcacg cgcgccgtat gatcccggat ctgcatcgta tgtttaaact
     8761 gtgtgtagtt gtaaaatgac acttcgatgc gcgtcaccga aatccatcga cggctctgcg
     8821 acttgtatgt acgatacacc gataaaaaga tggcttgcac ggactcgacc gcgtcggttg
     8881 gaacaagcgt agcctgcgct tccgtacggt tgagccgagg aagatgatgc agatgaaagt
     8941 ctaattgatg cagtttggcg tctcccatat tgccaaccta agaacgggca tgcaaaaaag
     9001 caaggctgag ctgtaatgaa gcaagttcgc ttgacgagtt gaccgaaagc atcgtattcg
     9061 agccgactcg caccaaaaaa gcaccaataa cccattcctt tttcaatcac agcttaaacc
     9121 agctattgct ccagcatgag acagtccctc tgccatgata cattaactta tatactacac
     9181 ctttatgtaa acaatcctaa gaatttgaaa taaaacaact tatgcttacc gaaaaatacg
     9241 gcataacatt tgttaataaa taaactgagc aaaaaacaat aactttgtac aagaattaga
     9301 cgatgcatac tatggaaatc atgtcggaaa acattcaagg agacgaaatg gcgagcaagg
     9361 gcagagtcgc tttacgggct ttgggattta tgatactggc gctgatcatt gcctcgattg
     9421 ccgtaatatt caatgcacct tcgatactga tgggtattac cgggattctt tttccaatcg
     9481 ggctttttat catgttgctt gtcgcatttt accttttgat tcttagcacc aaaggctgaa
     9541 gtattacatc cgttacaata ttgctatatc gaattttaat ttttcccgtt ctttatctat
     9601 agtttatcca gatgtttttc agagaatagt tcttactctg aattccggat atttccctct
     9661 tttgattttg cacattcgtt tctttttttc tatactcttc ttaaaaaatc ccttcaaaaa
     9721 tgcctgtatc ctcgagtaaa acacttcgac gccttgatta ttttttcatt ctccgaccta
     9781 ttttattcat tcccggttgg actacgacat tagcaggata tatggcggct acaggtcaag
     9841 cgaagcattg gccggtatgg aacggattat ggtatccgga tatgatggga ctctttattt
     9901 ccacggccat gttgatgggc agcggttttt tggtaaatca gctgcgcgat atcgaaaccg
     9961 atcgtattaa taaaaaactt ttttttcttt acgacggttc agtaaatccc cgcatactta
    10021 ttttacaatc cattttatta tccgccggtt gtttagtgat ggcttcattt tggggtgaac
    10081 gcatgctgat cgttcatttt ttcggctggc ttttgattgc gattttatac aatctccctc
    10141 cgtttcgtct caaagatcgg gccgtgacgg gacttttagc gaatgccggt atgggtgttt
    10201 ttgcattggc ttacggttgg gcgctgagcg atcaatcctg gcttttattt ttgataacgg
    10261 gatttcccta ttttattttc aatggagcgc tttgtgttct gacgatgatc cccgacacgg
    10321 aaggcgatgc ggccacatcc aaaaaaacat tcaccgtgcg ttacggcgtt acgtttagta
    10381 tgtatacggc ttttgttctt ttttgtatag ccgctatcac ggctttcatt ctggaccaat
    10441 acgtgctgct cgtcgttttg gtatccgtca tcccgatgtt tgtttttatg gtcattcgta
    10501 aaacaaaagc cgatgcaatt cgtgctttga aattcagcat attttttttt acttctttgg
    10561 tcgcaatgta ttttcctttt tatattatcc tgttgatctt tctttttgta tgtacgcgtt
    10621 ggtatttcaa aacgcggttt gattttgatt atcccaattt acaaggccgg tagttatgcg
    10681 tacctttttt tcatac
//
LOCUS       DAOQNQ010000089        10683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2219_length_10683_cov_5.822732, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000089 DAOQNQ010000000
VERSION     DAOQNQ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10683
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2219_length_10683_cov_5.822732"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3291)
                     /locus_tag="PK754_07255"
     CDS             complement(<1..3291)
                     /locus_tag="PK754_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNB09338.1"
                     /translation="MRHILIAILFFHTVVIFSARAQNASHPSSLIVKFRPKSQLSKTI
                     EDRKAWIQRIQTGTKEPIHIGSAFNNKHTFFDDTYIIQLAPNLTSDEINSWIANLSAQ
                     SDIEYVTPNHLFRLDDVGINDPLADSLYWIQRTAAKKAWALTQGRSGIIIGIIDSGIE
                     YDHPDLTAQIFRNAGEMGTDAWGNDKKSNGIDDDNNGYADDWQGWDFVDAPALAGDGD
                     ATDEDNDPSDENGHGTSVAGIAAATGNNHIGVIGMAFGCRVMNLRAGGKSGFLEEDDV
                     ARAIVYAADNGAQIINCSFGDREYGPLLRDAIRYASDRNIVIVASAGNSSSSALHYPS
                     SFDDVIGVSATAKDDFFAGFSNYGTRIDVAAPGVDILTTATNHGYRFFSGTSASAPLV
                     SGLAALMLSLDSTLSRDDVRATLQQSAEDIGEIGWEPYYGHGRIHAQRALLSIGGTGV
                     VIHYPAQNQGIIGNNIPVRITAAGRFMASVRLLIGSGTNPSSWTEVASSENHAFVNDV
                     LTDLDISDYSPGTYSLRLEVTNKDGSLNETVNRFFIDHTAPVISDFVVRPMLYGKYTG
                     YHIQFQTDDLCESYVRLRIAGSSESGFPLPIKTLSQNHSIFISTSDFRGQYEAAIEVI
                     NAAGDTAYYDNNGSYYPLNLTQTELPVSAFQIDTSFKMPAAYLYPRSTDMDNDGKPEL
                     TVNTYNNAGEFAWLRTYEFSGTSGHPVSLVHDYISNGVPRDIDDSASGGKMRLLAGMG
                     SSTVVYESVNATGLPISRTYKDSNDFWGSRFADMDGDGLPELIGKKGSAYQIRKNTAS
                     SWTTWQTLPNPTGGGNLVGVPGSAVGDFDHDGHQEILLGDYDGNIFIYELTGNSATLI
                     WTDSLHGVDANAFMESGDFDGDGFPEFAVGVHSEDPSSESDAVNSYWLWRIYKTTRNN
                     DYTIAWEQYFYGYAPLAQFPSGASSQDINNDQRPDLLLSLCPYAYILTYDASSQQYVW
                     YWHSDHVLSNTIAAADANHDGFSELYIGDGTATRRFVLQGLTAYELPSALAIFPSDTD
                     RIQIQWQTRPGIDSLLIFRGTHADTLALIVTIAANAETWTDSAVQKNKPYYYRIQARY
                     GNASSDVSG"
     gene            3402..4079
                     /locus_tag="PK754_07260"
     CDS             3402..4079
                     /locus_tag="PK754_07260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09339.1"
                     /translation="MHFEYSDLVTIGLLVLLEGLLSADNAIVLAVQVLPLPPDEQKKA
                     LRYGIIGAFVFRIIAVLLASYLINMPGVKIIGGIYLCWLALDHFRKHGEDQTQRTPKA
                     KQFFGLSAFWSTVIAVELTDIVFSVDSILAAVALSNKQWVVVTGGIIGIITMRMVAGV
                     FLQVIKKYPRLVDGAYIIVFLIGAKLFAEYFNIHVSKIVLFASIFLIMIISIMWSNWD
                     EKKKTNV"
     gene            4122..5873
                     /locus_tag="PK754_07265"
     CDS             4122..5873
                     /locus_tag="PK754_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB09340.1"
                     /translation="MSLLKLKNSRVYALVAWIAGFLISCGTSDLNNRGIVARIGNADS
                     ITAEELNNEIIKQRIVQSGKPVFIDKQRILLDMIELKIISLYSRQSQFSVPLNKVETE
                     SAVWTHVRGSVNQRFLENNFIDSAAVERYHAYSDEVLAVQAIVLYFNSNASSSDIKSQ
                     QNVKRRIDSIYSVCTSENFNELAKKHSEYAFQGVRNPLHPVKIAYKDMTFVMENAIFS
                     VAPGKPSKPVAIGDGYAIFRLNSELKNKLQNKTKLSPNKFYQMKSDLIEKIKKDDASL
                     YVELATSNINKEWINSNIRIDEKLIGEALREYRNSNASKTVPSFAAVAWVNENPIYTF
                     AQILEKVIIDRAPDKISDSLLISTTREEIRRYLYKKIYIEEFGVNEAYFEYLTKNIEL
                     EKSKRKLEKSIDDSLTSKMTDQILKSYYEKNREKFARHLDVGFSEIKCVSFEVASAAK
                     SLAVSGVVFEQIPEVVLKKNPHWLGKIKWVSNANLLNKPQCIRDRKLIFGGINDVSEV
                     FENEDGTYSIVVVRRRNTLTSYKSYGDAGREVFGLYKAEKKEKIIQSIILDIAKKHPI
                     SIYTQNMQSIGWSKDEH"
     gene            5863..6678
                     /locus_tag="PK754_07270"
     CDS             5863..6678
                     /locus_tag="PK754_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09341.1"
                     /translation="MNIKLFTFILILGFFLSCSGDTDYLARVNQAKLKEHDMDALHSS
                     VQLSPAIREKLIQDWVDQEALYHAALKAGIDKDPVFVTQMEKLRREILVQKFIDNDLE
                     QSVTISTSEIERYYSEHPGEFIYPDDHIRVQYFWTKNRNKIRDISVKFATMSRLRKRD
                     FMEIIAPTADDSDRVGTTPYLTRDKFDERVSKQLFAKTAIDEIIGPVQLSNGYFALWY
                     VIENLPKNSQVPLSEVADDIEARLRTLKRKARTAELVEKARKEAEIEYGERKP"
     gene            complement(6739..9180)
                     /locus_tag="PK754_07275"
     CDS             complement(6739..9180)
                     /locus_tag="PK754_07275"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HNB09342.1"
                     /translation="MHRNSWIRMLMHTWLMTLPMQFGLDVSAQLAESVSKKTHRSGGT
                     TFQPNAKTTLNVPKAESALIIDGEIEDTWLQGASFGNFVENGPVEGRQPLVETQGYVL
                     FDNDYVYFAFIAYDPEMHKLRANLSDRDRMFRDDFVGVNIDPFGAQTNGFEFFVNPLG
                     IQGDLSVDVNGNEDESFDAVWESAAKIYDDRWTVEIAIPFKSLRFPDRQDQNWLIHLW
                     RVYPRENRFLYSWIPIYRGKGNQYAQAGQIAMNMEKRPGKPIEFLPYALGSRDRFVKF
                     NGDSTKGIWSKSKYDKNFGFNLKYGVTSDMTLDLAYNPDFSQIEADATQISVNNAFAL
                     YYNERRPFFLEGRDVFFVDNDLNLMYTRSINDPLLLSKLSGKTDRLSYGYLFGMDENP
                     VYIVPQEESSSEPILTTKNAINNTLRAKYELAKGTYVGILGTDRRTSDGSNTVGTIDT
                     KIRLNDTYTLSGIVSASTTDEPDATSLYGSTDRFVAFGDSLTYGFNNEKFNGIATRVT
                     LDRTARHWGFFAWFNDRSPGFRSEQGFVTNNNVREVGAFNRYTFYMDDDHPLLTRVEP
                     RVQFNRKYNYDGKLKDWWINPQVFLQFKKQTYFWFSVAALNNENFAGKQFNNIHRISF
                     ESGTDAFQKLSGGVYGEVGSYINRSGNTEDIFNPLSKVKGTKLTVWATIRPISRLRND
                     FEFNSASFRKDFGGDLIDQQLIYRNTLQYQFTKRLFLRLIGEMGTINSHRAKRDKDTD
                     EIVLDVSNQQILTKTHEIFYAISPLMSYKINPFTVFYLGANFMGRNNLDFPNYDGANL
                     DRTNFFLKFQYLMQI"
     gene            complement(9262..9852)
                     /locus_tag="PK754_07280"
     CDS             complement(9262..9852)
                     /locus_tag="PK754_07280"
                     /inference="COORDINATES: protein motif:HMM:NF007980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNB09343.1"
                     /translation="MPHPLEDLNTLRTLIRHVYEDHKKRVLPAGQNTQAAVLIPLFLK
                     EQKIHVLLTKRTESVRTHKGQIAFPGGARDAEDTDLQITALRETEEEIGIPGKAVEIL
                     AETSPMVTPTQFHVTPFIGIVPYPFDWKINPYETESIIEIPLSHLLDERHHRKGFRMW
                     QDRTYEIHYFDYQEHTVWGVTGFILYEFLEELRPHL"
     gene            complement(9836..>10683)
                     /locus_tag="PK754_07285"
     CDS             complement(9836..>10683)
                     /locus_tag="PK754_07285"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HNB09344.1"
                     /translation="TEFLKHRLFDTLKDIIAAHTQGTRYNIHMDDDGLLYAGDSGVQL
                     TWMDAKIGDWVVTPRIGKPVEINALWYNALRIMAYFSNQLNHDDTAKAYEAMADRVKN
                     IFESLYWNEERRCLYDVVNSSGHDGSIRPNQIFAVSLPFPLLSDEKALAVINCVDAQL
                     RTPAGLRSLASSENGYTGVYAGDAYHRDAAYHQGTAWLWLIGPYMEAYLRVHHFSEEA
                     RTHIRTILQTIEPLIHDAGLGTLSEIADGDAPHLPKGCIAQAWSVAEVLRIMHMLESY
                     DAPSA"
BASE COUNT         2957 a         2274 c         2506 g         2946 t
ORIGIN      
        1 accggataca tcggatgagg cgttgccgta gcgcgcttgt attctgtaat aatacggttt
       61 gtttttttga acggctgaat cggtccacgt ttcggcgtta gccgcgatcg tcacgataag
      121 cgctaatgta tctgcatgcg tacctcgaaa aatgagcagt gaatctatac ccgggcgcgt
      181 ttgccattgt atttgaatgc gatcggtatc ggatggaaat atggccaacg cggagggtaa
      241 ttcgtaagcg gtcaggcctt gcaagacaaa tcggcgtgta gcggtaccat cgccgatgta
      301 tagttcggaa aatccgtcat gatttgcatc cgctgcagcg atagtattgc ttagcacatg
      361 atcgctatgc caataccaca catactgctg gcttgatgca tcgtaagtca ggatataggc
      421 atacggacac aacgacaata ataaatccgg gcgttgatcg ttattgatat cctgcgacga
      481 agcgccggat ggaaattgcg ctaacggcgc atacccataa aaatattgtt cccatgcgat
      541 ggtgtagtcg ttattccggg tagttttgta tatacgccat aaccaatagc tgttgacggc
      601 atcgctttcg gaagaaggat cttccgagtg aacgccgacg gcgaattccg ggaatccgtc
      661 cccgtcgaaa tcaccgctct ccatgaacgc attggcatcc acaccgtgca acgaatcggt
      721 ccaaatcagc gtcgccgagt ttccggttaa ttcgtagata aaaatatttc cgtcgtaatc
      781 gccgaggaga atttcttgat ggccgtcatg atcaaaatcg ccgacggccg aaccgggaac
      841 gccgaccaga ttaccaccgc cggtcggatt ggggagcgtt tgccaggttg tccaggatga
      901 agcagtgttt tttcgtattt gataagcgct tccttttttt ccgatcaatt caggtaaacc
      961 gtcgccgtcc atgtcggcaa accgcgatcc ccaaaaatca ttagaatctt tatacgttcg
     1021 tgaaatcggt aaaccggtcg cgttcacgct ttcgtacacg acggtggatg atcccatacc
     1081 ggcgagcaat cgcattttgc cgccggaagc gctgtcatct atatcgcggg gtacgccgtt
     1141 ggaaatataa tcatgaacta aagaaacagg atggccgctc gttcctgaaa attcatacgt
     1201 gcgtaaccac gcaaattctc cggcgttatt atacgtattc accgtcaatt ccggttttcc
     1261 gtcgttatcc atatcggtag aacggggata caaataggcg gcgggcattt tgaaagaagt
     1321 gtctatttga aatgccgaaa ccggtaactc cgtttgcgtg agattgagcg gataataact
     1381 tccgttatta tcgtaatacg cggtatctcc ggccgcgttg atcacttcga tagcggcttc
     1441 gtactggccc cggaaatcgg atgtgctgat gaaaatactg tgattttgtg aaagcgtttt
     1501 gatgggcaag ggaaatcccg attccgatga accggcgatg cgcaatcgta cataactttc
     1561 gcacagatca tccgtttgga actgaatatg ataccctgtg tatttgccat acaacatagg
     1621 gcgcactacg aagtccgaga tcaccggcgc ggtatgatca ataaaaaaac gattcaccgt
     1681 ttcgttcaac gagccgtctt tgttagtcac ttcgagtctc agcgaataag ttccgggcga
     1741 ataatcactt atatccaaat cggtgagcac atcattgaca aacgcatgat tttccgacga
     1801 cgcgacttcc gtccacgaag aagggttcgt gccgctaccg atcaacagac ggacggacgc
     1861 cataaaacgc ccggccgcag taatgcggac agggatgtta tttccgataa taccctgatt
     1921 ttgtgcaggg taatgaataa cgacgcctgt gccgccgata gacaacagtg cgcgctgggc
     1981 atggatacga ccatgtccat agtatggttc ccaaccgatt tctccgatat cttcggcgct
     2041 ttgctgtaac gtcgcacgta catcatcgcg ggaaagcgtg ctatccagcg acagcatcag
     2101 cgcggccaaa cccgatacca agggggccga agccgaagtg cctgaaaaaa aacgataccc
     2161 atgattcgtc gccgtcgtta gaatatctac gcccggtgcg gccacgtcaa tacgcgtacc
     2221 ataatttgag aaaccggcaa aaaaatcgtc tttggcggta gcgctgacac cgatgacatc
     2281 atcaaacgat gacggataat gcagtgcaga gctggacgaa ttgccggcgg aagcgacgat
     2341 cacgatattg cgatcgctcg cgtaacgtat cgcatcgcgc aataaaggtc catattcccg
     2401 atctccaaag ctacagttga tgatttgtgc gccgttatcc gctgcgtaaa cgatagcccg
     2461 cgccacatcg tcttcttcga gaaaacctga tttccctccg gcgcgcagat tcatgacacg
     2521 gcatccgaac gccataccga tcacgccgat atggttatta ccggtagcgg cggctatgcc
     2581 cgctacggag gtgccatgcc cgttttcatc agaagggtca ttatcttcat ccgtggcatc
     2641 gccgtcgcca gcgagcgcag gggcgtcaac aaaatcccac ccttgccagt cgtcggcata
     2701 tccgttgtta tcatcgtcta tgccgttgga ttttttgtcg tttccccatg cgtcggttcc
     2761 catttcaccg gcgttacgaa agatctgagc ggtaaggtcg ggatgatcgt attcgatacc
     2821 ggaatctata atcccgataa tgatgccgga gcggccttgc gtaagagccc aggctttttt
     2881 agcagcggtg cgttgtatcc aataaagact gtcggcgagc gggtcattga tacctacatc
     2941 gtccaatcga aacagatgat tgggcgtaac gtattcaata tcagactgtg cggacaagtt
     3001 ggcgatccaa gagtttattt catcggatgt aaggttcggc gcaagttgta ttatataggt
     3061 atcatcaaaa aaagtatgct tattgttgaa agcggagccg atatgaatcg gttcttttgt
     3121 gccggtttgg atacgctgta tccatgcttt gcggtcttcg attgttttgg ataattgact
     3181 tttcgggcga aattttacaa taagtgatga gggatgcgat gcgttttggg cacgagcaga
     3241 aaaaatgaca accgtgtgaa aaaataatat agcaatcaat atatggcgca tgaaatatta
     3301 tactaaaaga cacgggacgg tgcaacgtaa ttgcgcttga tagttctatt tcacaacgat
     3361 atattcctcg gcttttatat gaaaccaaat gaggatatgt tgtgcatttt gaatactcag
     3421 atctcgtaac catcgggctg ctggtcttac tggaaggttt gcttagcgcc gataacgcaa
     3481 ttgttttagc cgttcaggtt ttgccgttac cgccggatga acaaaagaaa gcgttgcggt
     3541 atggtattat cggtgcgttt gtatttcgga tcattgccgt attattggca tcttatctga
     3601 tcaatatgcc gggggtgaag atcatcggag ggatatattt gtgctggctc gccttggacc
     3661 actttcgtaa gcacggtgaa gatcaaacgc aacgtacacc taaagctaaa caatttttcg
     3721 gcttgtcagc cttctggagt acggtgattg ccgttgagtt gacggatatt gttttttccg
     3781 tagattcgat tttagccgcg gtagctttgt ccaataagca gtgggttgtc gttacgggtg
     3841 gcattatcgg aatcatcaca atgcgaatgg tcgcgggtgt ttttttgcaa gtgataaaaa
     3901 aatatccgcg tttggtggac ggcgcctata tcatcgtttt tcttatcggc gccaaattat
     3961 ttgccgagta ttttaatatc catgtttcga agattgttct ctttgcatcc atttttctga
     4021 ttatgatcat cagtattatg tggtcgaatt gggatgagaa gaaaaaaacg aatgtttagt
     4081 tgttaactga gtgacagtgt tttattgtta aggaattaga tatgagttta ttaaaattaa
     4141 aaaattcgcg cgtttatgct ttagttgcat ggattgccgg tttccttatt tcttgcggga
     4201 catcagacct gaataaccga ggcatagtag ctcggatagg caatgccgat agtataaccg
     4261 ccgaagaact gaataacgaa attattaaac aacggattgt gcaatccggg aaaccggttt
     4321 ttattgataa acaacgtata ctgctcgata tgattgagtt gaagattatt agtttgtatt
     4381 ctcgccaatc tcaatttagt gtgcctttaa ataaagttga aactgaatcg gctgtatgga
     4441 cgcatgtcag aggaagcgtg aaccaacgct ttttagaaaa taattttata gactcggcgg
     4501 ctgttgaaag ataccatgca tattctgatg aagtgttggc tgtgcaagcc attgtgctat
     4561 attttaattc caatgcctcc agttcagata tcaagtcaca gcaaaatgtc aaacgccgca
     4621 tcgattcgat ctattcggtt tgtacatccg aaaattttaa cgaacttgca aaaaaacact
     4681 cagagtacgc gttccaaggt gttcgcaatc cgttgcaccc agtgaaaatc gcatacaagg
     4741 atatgacttt tgtgatggag aacgctattt ttagcgttgc tcccggtaaa ccgtcaaagc
     4801 cggttgctat tggtgatgga tatgcgattt ttcgacttaa ctccgaatta aaaaacaaac
     4861 tacaaaacaa aacaaaatta tcgccgaata aattttatca aatgaagagc gatcttatcg
     4921 aaaaaattaa gaaggatgac gcttcattat atgtagaatt agctacttcg aatattaata
     4981 aggaatggat taattctaat attcgtattg acgaaaaatt aattggagaa gcgttgcgtg
     5041 aatataggaa ttcaaatgct tcaaagacgg taccgtcttt cgcagcagtc gcttgggtta
     5101 acgaaaatcc gatctataca ttcgctcaaa ttttggaaaa agtgataatt gatcgagcac
     5161 ctgataagat ttcagatagt ttattgattt ctacaacgcg tgaagaaatt aggaggtatt
     5221 tatataaaaa aatatatata gaagagttcg gagttaatga agcatatttt gagtacttaa
     5281 caaaaaatat cgaacttgaa aagtcaaaac gcaaacttga gaaatcgata gatgattcgt
     5341 tgacaagcaa gatgacggat caaattttga agagttatta tgaaaagaat cgcgagaagt
     5401 ttgcgcggca cttagatgta gggttttccg aaatcaagtg cgttagtttt gaggttgcgt
     5461 cggccgctaa atctttagcc gtatcgggtg ttgtgtttga acaaataccc gaagtcgtgt
     5521 taaagaagaa cccgcattgg ttaggaaaaa tcaagtgggt atcaaacgca aatcttctga
     5581 acaagccaca atgcattcgc gatcgaaagt tgatttttgg cggcattaac gatgtaagtg
     5641 aagtttttga aaatgaagac ggtacgtatt cgattgttgt tgtgcgtcgg cgtaatactc
     5701 taacaagtta taaatcgtac ggcgatgcgg gtcgcgaagt tttcggattg tataaggccg
     5761 agaaaaaaga aaaaattatt caaagtatta ttttagatat agccaaaaag catccgatca
     5821 gtatttatac acagaacatg cagagtattg ggtggagcaa agatgaacat taagttattc
     5881 acctttattt taatactggg gtttttttta tcctgttccg gtgacacgga ttaccttgcg
     5941 cgcgtcaatc aggcgaaatt gaaagagcat gatatggacg ccttgcattc gtcagtacag
     6001 ttatcgccgg cgatacgtga aaagttaatt caggactggg tggatcagga agcgctttat
     6061 catgcggcgt tgaaagcggg tattgataaa gatccggttt tcgtaacgca aatggaaaag
     6121 ttgcgacgtg aaatcctcgt tcaaaaattt atagataacg atttagaaca aagtgtaacc
     6181 atttcgacgt cggaaattga acgttattat agtgagcatc ccggagaatt tatctatccg
     6241 gatgatcata ttcgggtgca atatttctgg acaaaaaatc gaaataaaat ccgcgatatc
     6301 agtgtaaagt ttgccacgat gtcgcgcctt cgtaaacgcg attttatgga aattattgcg
     6361 ccgacggctg atgacagcga tcgtgtcggt acaacaccct atctgacgcg cgataagttt
     6421 gatgaacgcg tcagcaaaca actttttgcc aaaaccgcaa ttgacgaaat catcggtcct
     6481 gtgcaattgt ccaatggcta ctttgcctta tggtacgtga tcgagaacct tcctaaaaac
     6541 tcgcaagtac ctctgtccga agtggcggat gatatcgaag cgcgtctgcg aacgcttaaa
     6601 cgcaaagcgc gaacggcgga acttgtagaa aaagcgcgca aagaggccga gatcgaatac
     6661 ggcgaaagaa aaccataagt acaaatacaa aacccctttt catacgaaaa ggggttttaa
     6721 atttctaaac aacaagtttt atatctgcat caaatactga aattttaaaa agaagttggt
     6781 gcggtcgagg ttcgcgccgt cataatttgg aaaatctaaa ttatttcgac ccataaaatt
     6841 ggcgccaaga taaaacaccg taaaagggtt gatcttgtaa ctcataagtg gcgatatggc
     6901 gtagaaaatt tcatgtgttt tagttagtat ctgttgattg ctcacatcaa gtacgatttc
     6961 gtcggtatct ttatcacgtt ttgcacggtg tgaattgata gtgcccatct ctccgatcaa
     7021 tcgaaggaac aaccgttttg tgaattgata ctgcaatgta ttacgataga tcaattgttg
     7081 atcaatcaga tcaccgccga aatcttttcg gaagcttgct gaattgaatt cgaaatcatt
     7141 gcgtaaacgg ctgatcggcc gaatcgtagc ccaaactgtc aacttggtgc ctttgacttt
     7201 ggataatgga ttgaatatat cttctgtatt gccggagcga ttgatatagc taccgacttc
     7261 accgtagacg ccaccggaca gtttttgaaa ggcatccgta ccggattcaa atgaaatgcg
     7321 atgtatatta ttaaattgtt tgccagcaaa attttcatta ttgagtgccg cgacgctaaa
     7381 ccaaaagtaa gtttgttttt tgaattgtaa aaacacctga ggattgatcc accagtcctt
     7441 cagtttaccg tcgtaattgt atttgcgatt gaactgcaca cgcggttcaa cacgggtcaa
     7501 tagcggatga tcgtcatcca tataaaaagt gtatcggtta aatgcaccga cctcgcgtac
     7561 gttgttatta gtgacgaaac cttgttcgga acggaaaccc ggtgagcgat cattgaacca
     7621 agcgaaaaac ccccagtggc gagctgttct gtccaaagtt acacgtgtgg caataccgtt
     7681 gaatttttca ttgttaaatc catacgttaa ggaatcgcca aacgctacaa atctgtcggt
     7741 gctcccgtac agcgacgttg catccggttc atccgtagta ctagcggaga caataccgga
     7801 aagagtgtag gtatcattga gtcgtatttt agtgtctatc gtgccgacgg tattcgaacc
     7861 gtcggaagtc cggcgatctg ttcctaaaat gccgacatag gtgcctttgg caagttcgta
     7921 tttggcgcga agtgtattat tgattgcatt tttggtcgtt agaatgggtt cgctactact
     7981 ttcttcctga ggtactatat atactgggtt ctcatccatg ccaaataaat agccgtagct
     8041 tagtcggtct gttttcccag acaatttact aagtaataaa ggatcattga tgctgcgtgt
     8101 gtacatcaga ttgaggtcat tgtccacaaa aaaaacatcg cgtccttcaa ggaaaaaagg
     8161 tcgcctttcg ttatagtaca atgcaaaagc attattgacg ctgatctgcg ttgcgtcggc
     8221 ttcgatctga ctaaaatcgg ggttatatgc taagtctaaa gtcatatccg aagtcactcc
     8281 gtatttcaaa ttgaaaccga agtttttatc gtatttactt ttactccata tccctttggt
     8341 tgagtcgccg ttaaatttga caaaacgatc acgcgaacct aaagcataag gtaaaaattc
     8401 aatcggtttg ccgggacgtt tttccatatt catcgcaatt tggccggcct gtgcatattg
     8461 atttcctttt ccccggtaaa tcggtatcca agagtagaga aaacgatttt cgcgaggata
     8521 aactcgccag aggtggatta gccaattttg atcttgtctg tcgggaaaac gtaagctttt
     8581 gaacgggatg gcgatttcta ctgtccaacg atcatcataa attttagctg ccgactccca
     8641 tacggcatca aaactttcgt cctcatttcc attgacatcc acggaaagat caccttggat
     8701 accgaggggg tttacgaaaa actcaaaacc attagtttgt gcgccaaaag gatcaatgtt
     8761 gactccgacg aaatcatcgc gaaacatacg atcccggtcg ctcaggttgg cccgcagttt
     8821 atgcatctcc ggatcatagg cgataaaagc aaaatagaca tagtcattat caaacaaaac
     8881 ataaccttgc gtttccacta aaggttgacg cccttcaacc ggaccgtttt caacaaaatt
     8941 tccaaatgat gcgccttgca accatgtatc ttcaatttca ccgtctatga tgagcgccga
     9001 ttcggctttc ggtacattga gtgtcgtttt agcgtttggc tggaaggtgg ttccacccga
     9061 gcgatgcgtt tttttgctta cagactcggc cagctgtgca gatacatcca aaccaaactg
     9121 catcggcaaa gtcataagcc aggtatgcat caacatgcga atccaagaat tgcggtgcat
     9181 aagattcctt ccggtatgaa gaagtagggt gactgttacg ccctgtcata cggtcgggcg
     9241 gctaaaaggt tgcaggattt tttacaaatg agggcgaagt tcttctaaaa actcatacag
     9301 tataaacccg gtaacgcccc agacggtgtg ttcttggtaa tcgaaataat ggatttcata
     9361 cgtacgatct tgccacatac gaaatccctt gcgatgatgg cgttcatcca aaagatggga
     9421 cagcggtatt tcgatgatgg attcggtttc atacggattg attttccaat caaacggata
     9481 cggcacgatg ccgataaaag gggttacatg aaattgagtc ggcgtgacca tcggactggt
     9541 ttcagctaaa atttcgacag cctttccggg tatgccgatt tcttcttctg tttctcgaag
     9601 tgcagtaatt tgtaagtccg tatcttccgc atcccgcgca ccgccgggga aagcaatctg
     9661 acctttatgg gtgcgtacgc tttcggtgcg tttggttagt aaaacgtgaa ttttttgttc
     9721 tttgagaaag agcgggatta aaacagcggc ttgcgtattc tgtccggcgg gcaatacgcg
     9781 ctttttgtga tcttcgtaca catgacgaat cagagtgcgc aatgtattca aatcttcaag
     9841 cggatggggc atcgtaggat tctaacatgt gcataatgcg caatacttcg gcgacggacc
     9901 aagcctgtgc gatgcagcct ttgggtaagt gcggcgcatc cccgtcggcg atttccgata
     9961 atgtacctaa gccggcgtcg tggatcagcg gttcgatcgt ttgcaatatc gtccggatgt
    10021 gtgtgcgcgc ttcctcagaa aaatgatgaa cgcgcaagta agcttccatg taaggtccga
    10081 taagccacag ccatgccgtg ccctgatgat acgccgcatc acgatgatac gcatcgccgg
    10141 cgtaaacgcc cgtataacca ttctcagatg aagcaaggct gcgcaggccg gctggcgttc
    10201 tcagttgcgc atcaacgcag tttatcacgg ccagagcttt ttcatcggat aaaagcggaa
    10261 aaggcaaact gactgcaaat atctgattgg ggcgaatact cccgtcatgg ccggacgaat
    10321 tgactacatc atagaggcac cggcgctctt cgttccaata taaggattca aaaatatttt
    10381 ttacccgatc ggccatcgct tcatatgctt tagccgtgtc gtcatggtta agctggtttg
    10441 aaaaataagc cataatccgg agtgcgttat accacagggc gttgatttcg accggtttgc
    10501 cgatgcgtgg tgtaacaacc caatctccga ttttggcatc catccatgtc agttgtacac
    10561 cgctgtcgcc ggcataaagt agcccgtcat catccatgtg aatattataa cgggtgcctt
    10621 gcgtatgtgc cgcgatgata tctttcagcg tatcaaacaa acgatgcttc agaaactctg
    10681 tat
//
LOCUS       DAOQNQ010000090        10642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2234_length_10642_cov_5.513365, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000090 DAOQNQ010000000
VERSION     DAOQNQ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10642
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2234_length_10642_cov_5.513365"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="PK754_07290"
     CDS             complement(<1..580)
                     /locus_tag="PK754_07290"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB09345.1"
                     /translation="MQVKSRILFLVDDTGVAEKVMAQLVIQGVEAEHKSIIPSLESLE
                     CIQYFHPDAVVCVNTSYGLITHCRRIFDYASIIVTGEHGEFENLSRHFQWIGSDQNND
                     HLSSLIIEVLHKNATERAELPKIMERDIIESSLDVIVAVDKQRRIVVFNKAAEKTFGY
                     LRSEVLGKSIRMLYADDSQSDRVVQSMKHSGKY"
     gene            complement(593..991)
                     /locus_tag="PK754_07295"
     CDS             complement(593..991)
                     /locus_tag="PK754_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB09346.1"
                     /translation="MNPTILCVDDDAEVLKSLTILFEEDYNVLTCIHPLKALELLATN
                     NDIRLILTDHRMPDMNGLQLLKRIRETMPERAFYTIIYSGYSEVEGEMDEMIRARVIN
                     EFLTKSCPPKLLLQAVASGIKKTETLAAPV"
     gene            complement(1065..1706)
                     /locus_tag="PK754_07300"
     CDS             complement(1065..1706)
                     /locus_tag="PK754_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09347.1"
                     /translation="MISESEINIRGMIKGLAIIPLFKFIQKHDLNWHAILTQLPDRCK
                     AELSLLIKSQYTLHDFFPTPQDGLKLFSALRWYPIVISNGFLVAIARQMHFQCGVSLE
                     RGYYAIGHFTATHDLSGLLGYILRWGNILQMLEMGEKIWKHYISEGELIVISKAEGRI
                     QFDIVCSVVLSEQQYVNQGWLAALLEKKEIAQYKVETMYLQLGGGTITHKIIW"
     gene            complement(1789..3753)
                     /locus_tag="PK754_07305"
     CDS             complement(1789..3753)
                     /locus_tag="PK754_07305"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNB09348.1"
                     /translation="MKCTFFLAALIVSTAGLSAQEKDYFDMTLEELLNVEVSVATQNK
                     LSAKESPGIISYITSDQIKVSGARDLLDVLRMVPGFDFNVTTWGAVIMSARGIADNGN
                     VSFLMDGFDLTERLYSDVYFGNHYDINQIERIEIIRGPGSVLYGGTAELAVINVITKK
                     ATDFNGGNGTVAGGLMSKTYGRANVSGSYAYSWDTDVNAFAHVFFGNANRSDRTYTDY
                     YGTSYAMKNNSGIQTYFLNGGASIGNFSFQIIVDQYNIQERDHYGITLPKKTTNHFHE
                     YLGSIKYKIHGQKWSITPSATYSHQTPWWVNNKTLDNEVWFTNWKANSDRLSMDIVAS
                     YDLNNKTNILAGANGYRDWAKYFNGSYGITGQDLSYRYWGAAAFAQIYNRNEIANVTL
                     GIRIDKHSEYGTVAVPRLALTKVMDQLHLKALYSRAFRAPGLEALRFSNGKIKPEYTT
                     AYEFETGYQWNKTLSTTVNIYDVMITDPMAYVYENNKDNYRNFGRFGTRGIEIMQQYK
                     SDLSAITFGFSHYVANKKSDVKNYQVIDYTNANPKLVNEQANLGVPQSKLSFNGLFKV
                     KKDVRISTTWVWYSSRYGYDAIDLNGNLVPKRYDPVHMLDIYVSYKSLGVGVYNIYNS
                     KYNFLMAYNGGHPPYPGMGREVYAKYEIKF"
     gene            complement(3787..4302)
                     /locus_tag="PK754_07310"
     CDS             complement(3787..4302)
                     /locus_tag="PK754_07310"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HNB09349.1"
                     /translation="MILTGTPIYSQSRVEIEESLHASIMLKALTYDSKMESANITVIS
                     RKNGNSNRSALVKSVDGKFIKNNAIAVNQLTYSDKVSFLKALETMKPGIIYLCDDLYT
                     SQILEISEACNNLKILTISGTPDFVRSGYVSMSVIDENQKPKIILSLKKLKSEGRNFQ
                     ASLLKLCKIID"
     gene            complement(4330..5751)
                     /locus_tag="PK754_07315"
     CDS             complement(4330..5751)
                     /locus_tag="PK754_07315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB09350.1"
                     /translation="MITNEKILALFDFFRHRKGNIGLLLGLSILFFGQQTTSYFQNLS
                     AVREQAIENARQNIDYLSEEDAPIALKLENTESIEKFNKYLSKDTIYFRSLGLHDASG
                     HDLFRYIKSNHPVDSLRFALTMQDSAEGLFLVEDYIIYSKKIIDELEGKVLGKALIAY
                     SIEEKHKELNYSFLLTGTLFGCILILILIMMIKSSYKIYRSEKELAQKNLMLKDSIGQ
                     IQTAQDQLIQSEKLSAIGLMVAGIAHEINTPKASISNYGIVFSRKLSKIAKSDDLVFI
                     KETCKNIEEEILPAIELCSERIGTIVDSLLNFTRIDHKEMQYSNISKIVEGVLIILKS
                     QNRSQVLIVNNIDTSISYVCYRGQLSQVFMNIISNAIQAILTGNPERTDGVVKLNAEV
                     TNTNLIIRIQDNGPGMSESIRKKIFDPFFTTKPVGKGTGLGLSISYDIIKKHNGSIEC
                     RSEPGVGTEFIITIPDRRIHQTT"
     gene            complement(5766..6305)
                     /locus_tag="PK754_07320"
     CDS             complement(5766..6305)
                     /locus_tag="PK754_07320"
                     /inference="COORDINATES: protein motif:HMM:NF019320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HNB09351.1"
                     /translation="MHGIVFLELRKFVEQNMGKDGWRNLLKQTEIEVDHYMPSQTYPD
                     SELYALVASACSMTGQPVNAILEAFGKFMVPNLVSVYGAFIDRKWGLIDVLENIDKTI
                     HNVVRRKNPGALPPALETHRIDIDRIEIVYRSERKMCSLGKGLILGLSELYRQNISYE
                     EATCMHTGADACRIRIIVQ"
     gene            complement(6316..6705)
                     /locus_tag="PK754_07325"
     CDS             complement(6316..6705)
                     /locus_tag="PK754_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09352.1"
                     /translation="MIDKPEILLVGVHPNFLNNLVRIFEVDFHVVSALGPMSAIEALS
                     ELQSIRVIIADAEYKTMNCLEFFKHISEQFPQRDFYKIVYAVSGDKNKIAMIEKSKND
                     FDAFVRGADSSKFIIHAVKQGLKCTTV"
     gene            complement(7290..7703)
                     /locus_tag="PK754_07330"
     CDS             complement(7290..7703)
                     /locus_tag="PK754_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09353.1"
                     /translation="MRNPIKFISFIFLIAISCSDDQSGHDNHSLDGDWTLAYTIIQYI
                     ATGDTIDVRYWDAKQIGLAITFGRDNTYTIYALLGDTIALQTNGMYSVISDNIVFDNL
                     IGTTQVMRFLKKESELCLVSYDYEVAETMFYRKKK"
     gene            complement(8194..10056)
                     /locus_tag="PK754_07335"
     CDS             complement(8194..10056)
                     /locus_tag="PK754_07335"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNB09354.1"
                     /translation="MNFTELFRSLIQKTTGVAGAEVFRRLCVEAKEALPFFEICWIGK
                     KDPLYERVTVLEFEKKDRIGSFPNSYMLSGQACEEVYKLPEPQVFDLRYSNVSVPVDI
                     PNGFYYGIPIIDMKGQVLGHFGVILSKKIDLGETEEWRIFLKKLRLEIERMEDDMRES
                     ETKNKLQAVLDSTMDGIVAFDEDYRILIFNDAAEQILKSEPGKNIGHSFKRFLTPDFR
                     SACDEYMVQCMQQKNNFQSLYFLNKHLKLRDGRGGVVEAEVNFSCIRYKNEMYFTVII
                     RDLAMQNQMQYQINQLKDEKTKLITEIKSFYNRDIAVGKSPAFEKVLDDIRKVSQTSS
                     TVLLTGETGTGKELIAATIHNTSKRQQNIFIKVNCTALPSSLIESELFGHEKGAFTGA
                     TSMRKGRFELADGGTLFLDEIGELNKDVQSKLLRVLQEREFERVGGSETIHVNVRIIA
                     ATHQNLLAKVRDGSFREDLFFRLNVFPIHVPPLRERISDLPLLAKYLIDKLSLNVGKN
                     VRFICNDALALLREYSWPGNIRELSNVLERAIILCDEETIRPSHIFLTQQQSKTENEM
                     QLVDMEIRHIKTILIKTRGKIAGRGGAAELLGINRSTLISKMKKLNIDHRQLSS"
BASE COUNT         3163 a         2216 c         1839 g         3424 t
ORIGIN      
        1 catacttacc actgtgtttc atgctctgca caacacggtc gctttggcta tcatcggcat
       61 atagcattcg aatacttttg cctaatacct cactgcgtaa gtacccgaat gttttttctg
      121 cggctttgtt aaatacaact atacgccgct gtttgtcaac ggccacgatc acatccaaag
      181 agctttcaat aatgtcgcgc tccatgattt taggaagttc agcacgttct gttgcgtttt
      241 tatgtagaac ttcaataatc aacgatgaca aatgatcatt gttttgatct gacccgatcc
      301 attgaaaatg tctcgacaag ttttcaaatt caccatgctc ccctgttaca ataatagacg
      361 cgtaatcaaa aattctccgg caatgcgtga taagcccgta cgatgtattg acgcatacga
      421 ccgcatccgg gtgaaaatat tgaatacact caagtgattc gaggcttgga attatagatt
      481 tatgctcggc ttcaacgcct tgaatgacga gctgtgccat tactttttcg gcaactccag
      541 tatcatcaac aagaaataaa atccgcgatt tgacttgcat acatctttcc ggttatacag
      601 gagccgcaag cgtttcagtt ttttttatac cactggcaac tgcttggagt aataatttcg
      661 gaggacatga ctttgtcagg aattcgttaa taactctcgc acgaatcatt tcatccatct
      721 ccccttccac ttctgaatac cctgaatata tgatcgtata gaatgctcgt tccggcatgg
      781 tttctctaat tcgtttcaac agttgcagtc cattcatatc cggcatacga tgatcggtaa
      841 gtatcaaacg aatatcatta tttgttgcta acagctccaa cgcttttaaa gggtgaatgc
      901 aggtgagcac attataatct tcttcaaaaa gaatggtaag ggactttagc acttccgcat
      961 catcatcaac gcataagatc gtgggattca tgaattctcc ttatctaaat atattaggta
     1021 actaattgtc atacatcatt tcttctatca tcagctcgca cactttacca tatgattttg
     1081 tgtgtaatcg ttcctccgcc aagctgaagg tacatggttt caactttgta ttgtgcaatc
     1141 tctttctttt ccagcagcgc cgccaaccat ccttgattga catattgttg ttcagacagt
     1201 acgaccgaac atacaatatc aaattggatc cggccttctg ctttgctaat cacaatcaat
     1261 tcaccctcgg agatataatg tttccatatt ttttcaccca tttcgagcat ttgaagtata
     1321 tttccccatc gcagtatgta cccgagcaaa ccggaaagat cgtgggtagc cgtaaaatgg
     1381 ccgatagcat aataacctcg ttcaagcgat acaccacatt gaaaatgcat ttgccttgca
     1441 atagccacga gaaatccgtt ggatataaca atcggatacc atcgcaacgc agagaacaac
     1501 ttcaatccgt cctgcggtgt cggaaaaaaa tcatgaagcg tatattgact tttgattaat
     1561 agtgataact ctgctttgca gcgatcgggc aactgggtga gtatcgcatg ccaattcaaa
     1621 tcatgttttt gaataaactt aaacaaagga ataatggcta agcctttgat catacctcga
     1681 atgttaattt cagattcaga aatcatagtt tatttaggct tagcgcttta gtttagattt
     1741 atctatcaac tgttattttt ttgatgataa actcacatcg gtcgatcatc aaaatttaat
     1801 ttcatatttc gcatagactt cacgtcccat accgggatac ggtgggtgac caccgttata
     1861 agccatgaga aaattatatt tggagttgta tatgttatat actccgacac ccaaactctt
     1921 ataactaaca taaatatcca acatgtggac gggatcataa cgtttcggaa ccagattccc
     1981 gttcagatca atggcatcat atccatagcg cgatgaatac caaacccatg tagtgctaat
     2041 acgaacatct tttttgacct taaacagacc gttgaacgat aacttggatt gcggtacacc
     2101 cagattagct tgttcattta ctaatttggg attagcatta gtataatcta tcacctgata
     2161 atttttgacg tcgctctttt tattggcgac ataatgtgag aatccaaacg taatcgcgct
     2221 cagatcggat ttgtattgtt gcatgatttc gatgccgcgt gttccaaacc gtccgaaatt
     2281 tcgataatta tccttattat tttcataaac gtacgccatc ggatcggtaa tcatcacatc
     2341 atatatattt accgttgtgg atagtgtttt attccattga taaccggtct caaactcata
     2401 ggccgtagta tattcgggtt tgattttacc gttgctaaaa cgcaaagctt ccaagccagg
     2461 tgcacgaaaa gcccgactgt ataatgcttt taaatgcaat tgatccatta ctttggtaag
     2521 cgctaaacgt ggtacagcca ccgtcccata ctcactgtgc ttatcaattc gtatgcccaa
     2581 agtaacatta gctatttcat tacggttata aatttgcgca aatgcagctg cgccccaata
     2641 acgataagat aaatcctgac cagtaatacc gtacgatcca ttaaaatatt ttgcccagtc
     2701 acgataaccg ttagcgcccg ccaaaatgtt ggttttgtta tttaaatcgt aagaagcgac
     2761 aatatccata ctcaaacgat cgctattggc tttccaattg gtaaaccata cttcgttatc
     2821 gagcgtttta ttgtttaccc accaaggcgt ttgatgggaa tacgtagccg acggagttat
     2881 actccacttt tgaccatgaa ttttatattt aattgagccc aagtactcgt gaaagtggtt
     2941 agtcgttttt ttcggcaatg tgattccgta atgatctctt tcctgtatat tgtattgatc
     3001 cacaataatc tgaaaactga aatttccgat ggatgctcca ccgtttaaaa agtatgtttg
     3061 aatgcctgag ttgtttttca tggcgtatga tgttccgtaa taatcggtat aggtacggtc
     3121 actgcgattg gcattgccaa aaaaaacatg tgcaaatgcg tttacatccg tatcccagga
     3181 atacgcataa ctgccggaca cattagcgcg cccatatgtc ttcgacatca atcctccagc
     3241 tacggttcca ttgcctccgt tgaaatctgt tgctttttta gtaatgacgt tgatcactgc
     3301 aagttctgct gtaccgccat acaacacaga acccggaccg cgaataattt ctatccgttc
     3361 aatctgattg atatcgtagt gattcccaaa ataaacatcc gaataaagtc gttctgtcaa
     3421 atcaaatcca tccattagga atgacacatt gccattatcg gctataccgc gcgcactcat
     3481 aatgacagcc ccccaagttg taacgttaaa atcaaaaccg ggtaccatac gtaaaacatc
     3541 cagcaaatcc cgagcaccgg acacctttat ttggtcagaa gttatgtaag atataatccc
     3601 aggagactct ttggctgaca gtttattttg cgtagctacg ctgacctcga cattaagcaa
     3661 ctcttctaac gtcatatcaa aataatcttt ttcctgggca ctaagccctg cagttgatac
     3721 gatcaaggcg gctaaaaaaa aagtgcactt catacctaat ccttagtatt tgaattttta
     3781 aaagagtcaa tcaattattt tacatagttt caaaagtgaa gcttgaaaat tacgaccttc
     3841 ggattttaac ttttttaaac tcagtattat ttttggtttt tgattttcat caataacgga
     3901 cattgataca tagccgctgc ggacgaaatc cggagtcccg gatatcgtca atatttttaa
     3961 attattacaa gcctcgctaa tctctaaaat ttgagacgta tacaaatcat cgcataaata
     4021 aattatgccg ggcttcatcg tttccaaagc tttcagaaaa ctaactttat cggaataagt
     4081 aagttgattg acagcgatcg cattattttt gatgaattta ccatcaacac ttttaaccaa
     4141 agcagaacga ttggagtttc cgttttttct tgagattaca gttatattgg ccgattccat
     4201 ttttgaatcg taggttaatg cctttagcat aatactggcg tgcaaagatt cttctatttc
     4261 aacccgagac tgcgaataaa taggcgtacc ggttaaaatc aagatgaagc agattaatag
     4321 tttcataaat tatgtcgttt gatggatacg acgatcagga atagttataa taaattcggt
     4381 gccaacgccg ggttcgcttc tgcactcgat ggaaccatta tgtttcttta tgatatcata
     4441 tgagattgaa agcccgagtc cggttccttt tccgaccggt ttggtcgtaa aaaagggatc
     4501 aaatattttt tttcggattg attcggacat tcccggacca ttatcttgaa tacgtataat
     4561 caaattggta ttggttacct ctgcattgag ttttacaact ccatccgtgc gctcgggatt
     4621 acccgttaga atcgcttgta tggcgttgga aataatattc ataaaaacct gactcaattg
     4681 accgcggtag cacacataac ttatggacgt atcaatgtta ttgacgatca agacttggct
     4741 tctattttga cttttaagaa tgattaaaac accttccact atttttgaaa tatttgaata
     4801 ttgcatttct ttatgatcaa tacgtgtgaa gtttaacaag ctgtcaacaa tcgtgcctat
     4861 acgctcactg cacaattcaa ttgccggcaa aatttcctct tcgatatttt tacatgtttc
     4921 tttgatgaac actaaatcat ccgattttgc gattttggat aattttctgc tgaacacaat
     4981 tccataatta cttatgctgg ctttaggtgt attgatttcg tgtgcgatcc ctgccaccat
     5041 aagaccgata gccgataatt tttcagactg tatgagctga tcctgagccg tttggatttg
     5101 gccgatactg tctttaagca tcaggttttt ttgcgccagc tctttctcgc ttcggtaaat
     5161 tttataagac gatttgatca tcatgatcaa tatgagtatc aaaatgcacc caaaaagggt
     5221 tccagtaagt aaaaatgaat agttaagttc tttgtgtttt tcttcgatcg agtaggctat
     5281 cagcgcttta cctagtactt ttccctccaa ttcgtctata atttttttac tgtaaattat
     5341 ataatcttcc accaaaaaaa gaccctctgc agaatcttgc attgtaagag caaatcgcaa
     5401 actgtctacc ggatgattac tttttatata tctgaaaaga tcatggcctg acgcatcatg
     5461 taacccgagt gaacgaaaat aaatcgtatc tttacttaag tatttattaa acttttcaat
     5521 gctttccgta ttctcaagtt tgagagctat aggcgcatct tcttcggaaa ggtagtctat
     5581 gttttgccgt gcattctcta tagcttgttc tcttaccgca ctaaggtttt ggaagtaact
     5641 tgttgtttgt tgcccaaaaa aaagaatact caaacccaac aataaaccga tgtttccctt
     5701 gcggtgtcta aagaaatcaa acagggccaa tattttttcg tttgttatca ttaaactctt
     5761 aattcctatt gtacgattat ccttatgcgg catgcgtcgg cccctgtatg catgcatgtt
     5821 gcttcttcat agcttatatt ttgtcggtag agttcgctaa gaccaaggat taaacctttt
     5881 ccaagcgaac acatttttct ttcggatcta taaacaattt ctattcggtc aatatcaatg
     5941 cgatgagtct ccaaagccgg aggcaacgcc ccgggattct ttctacggac aacattgtgg
     6001 attgtcttat ctatattctc aagaacgtca attagtcccc attttcgatc aatgaaagca
     6061 ccatacacag acacaagatt aggtaccata aatttcccaa acgcttcgag tatggcgttc
     6121 acgggttgac cggtcattga acatgcggaa gcaactaagg catataattc agaatccggg
     6181 tatgtttgcg acggcatgta atgatctact tctatttctg tttgtttcag taagtttctc
     6241 caaccgtctt tacccatatt ttgctcgaca aattttcgca actccagaaa cacaatccca
     6301 tgcataccca tctccttata ccgtagtgca tttcaaacct tgctttacgg catgaataat
     6361 aaacttcgat gaatcggcgc ccctgacaaa cgcatcgaaa tcgttttttg atttttcaat
     6421 cattgcgatc ttatttttgt cacccgaaac agcatagacg attttataaa aatcgcgctg
     6481 cgggaactgc tcagatatat gcttaaagaa ctctaaacag ttcatcgttt tatattcggc
     6541 atccgcaata atgactctaa ttgattgaag ctcggataag gcttcaatag cactcatagg
     6601 tcccaaagct gataccacat ggaaatcaac ctcaaaaatt cgcactaaat tattaagaaa
     6661 attcgggtgg acacctacaa ggagtatttc aggtttatca atcaattgct tttcctagta
     6721 aaactaattc tgcaacatta tcaccgacat agtgacaaac aacgtgccaa tattaaaccc
     6781 tttataatca atcttttttt aagatgcgga ttagtaaatg tttacatata aacatcaact
     6841 gttgatattt cgacaaaatg attgccttgt tagcctcgtg aaagtgctta acccgatgac
     6901 tttgtaattt ttacaaataa cagttagcct gatctggggc gaataaaaaa taccctgatg
     6961 aatgtttttg ttgttgattg atttgaattg agaacgaaga aatgtaatga aggctatatg
     7021 taatagattg gcttcagaaa ttcttcatcg gttatacaca tcaattttga accataaaga
     7081 tgtaactgtt gcattataaa cctttgtaaa tgccggcaaa tgtcctacat gatttcagac
     7141 atcttttgtt tttaaaacat ctcttgtcta cccttttaag atttccgcaa aacttttata
     7201 gagtagattc ggtattctaa cgtatataac gataatctaa ctcgattagg tttagacatg
     7261 atggctttgt ttcagcgatc acaaacgaac tattttttct tacgatagaa catggtttct
     7321 gcaacctcat aatcataact tacaagacat aattcagatt cctttttcag aaatcgcatg
     7381 acctgcgttg ttccgatcag attatcaaat acgatattat cagatattac tgagtacatt
     7441 ccattagttt gaagtgcaat agtatccccg agtaaagcat atatagtgta tgtgttatcc
     7501 ctaccaaagg ttatagccag acctatttgt tttgcatccc aatatcgcac atctatagtg
     7561 tcccccgttg ctatgtattg gataatcgta tacgccagcg tccaatctcc atctaagcta
     7621 tgattatcat gtccgctttg atcgtcagag cacgaaatgg ctattaagaa aataaaactt
     7681 atgaatttta taggatttct cactttggaa acgattaggt tactaacaca aggtgtatat
     7741 taaaatagtt gcaaaagtta agccaacaaa accgcatctg tttttatttg ttttatacac
     7801 cgctaattag ttttaaccgt tgaaactgcg acaagagtgt cgatatatcg ccagcgaata
     7861 tcaaacacaa aagcgagaca tgttttttat taaacacatg taatgaagtg aaaattcaca
     7921 ctcacatctt ttcttaaaaa aactgcaagg aaaatctatc ttaacgtcat gattagccga
     7981 tcatattcgc ctctgcattt ttaatcttta aacctcgatt caccattttc ggtaactacc
     8041 gtccgtaacc tcgtgtgatg tacatagaca tagcacaata gaaaaatgga catttaaaaa
     8101 aagaatacgc agctgatact ttcagacaga tagtcatacc ctcaaacggt aacgcgtcca
     8161 gccactgata tctattttta ttattatatg tatttaggag ctaagctggc gatgatcaat
     8221 atttagtttt ttcattttgg atatgagcgt tgatcgattg atgcccaaca gttcggcagc
     8281 acctccacga ccggcaattt tgccgcgagt ttttataagt atggttttta tgtggcgtat
     8341 ctccatatcg accaattgca tttcattttc agttttagat tgttgttgtg ttaaaaatat
     8401 atgactaggt cgaatagttt cttcgtcgca aagaattatt gctctttcta atacattcga
     8461 taattcacgg atgttgcctg gccatgaata ttcacgcaat aaggccagtg cgtcgttaca
     8521 aataaagcgg acattttttc caacattcaa agatagttta tctattaaat acttagccag
     8581 aagcggcaga tcgctgatac gctcacgtaa cggaggcaca tggataggaa acacatttaa
     8641 tctaaaaaac aaatcctcac gaaaagaacc gtcacgaact ttggctaata gattctgatg
     8701 tgttgcggca atgatcctta cattgacatg gatggtttcc gatcctccaa cccgttcgaa
     8761 ttcacgttct tgaagcaccc tcaaaagttt ggactgaacg tctttattga gctctccgat
     8821 ctcatcaaga aaaagagtac ctccatcggc taattcaaat cgccccttcc gcattgacgt
     8881 cgcaccggta aacgctcctt tctcatggcc aaacaattca ctctcaatca atgaactcgg
     8941 caacgccgta caattgactt tgataaatat attttgctgc cttttactgg tgttgtgaat
     9001 agtagccgca ataagttctt tgccggtacc cgtttcacct gttaatagta cagttgaact
     9061 ggtttgactg actttacgga tatcatcaag taccttttca aacgccgggc ttttacccac
     9121 cgcaatatcg cgattataaa aagatttgat ttccgttatg agtttagttt tttcatcttt
     9181 gagttgattg atctgatatt gcatttgatt ttgcatcgcc agatctctta taatgacagt
     9241 aaagtacatt tcatttttat atctgatgca cgaaaaattt acttccgctt ccaccacacc
     9301 accacgacca tcacgtagtt tgaggtgctt gtttaaaaaa tacaacgact ggaaattgtt
     9361 tttttgttgc atgcattgaa ccatgtattc gtcacaggcg gacctaaaat caggagttaa
     9421 aaatcgcttg aatgaatgcc ctatattttt tcccggttct gatttcaata tttgttctgc
     9481 agcatcgttg aatatgagta tacgatagtc ctcatcaaat gcgacgatgc catccatagt
     9541 cgaatcaagt actgcctgca atttattttt agtctctgat tctctcatat catcttccat
     9601 gcgctctatt tccaagcgca atttcttaag gaatattcgc cactcttccg tctcgcctaa
     9661 atcaattttt tttgacaaaa taaccccgaa atgccctaag acttgacctt tcatgtcaat
     9721 aataggaatt ccataataaa aaccatttgg aatatctacc ggcacgctaa cattcgaata
     9781 ccgcagatca aatacttgtg gttccggcaa tttatatacc tcctcacaag cttgacctga
     9841 taacatatat gaattaggaa atgacccgat tcgatccttt ttttcaaatt ccaaaacggt
     9901 tacgcgctca tacaatggat ctttctttcc tatccaacat atttcaaaaa aaggcaacgc
     9961 ctcttttgcc tcaacacaaa gtcgtctaaa cacttcagcc cctgctacac cggtagtctt
    10021 ttgaataaga gaacgaaata gttctgtgaa attcactttt actccttgtg caaatatttt
    10081 tacttagtaa gaccgaaagc aaagatataa atgtatacac aggataagcc tacataatga
    10141 tgtagagacg actttgtaca acctaaagaa tttgttaaac gatttttttg tttacgggag
    10201 aattgtatgg ctattgccac gtaaaaatct ttttgcactc gaaatatttg aatgctggat
    10261 atagtatcgg cagcaatggt atgataacaa attgaaagaa tgttcaagtt aacgcgtatg
    10321 cttctcggta attatcatat tactcaatat cttttacagg taaagacatt agcgtaaata
    10381 ctatgaaatg ctatcgagat taaactgaat aattaaacgc tagttataat tatgaatcca
    10441 tatcattact tgggatgatt cttacataat gccctcgatt tcaaaaatct ttgcctaatt
    10501 tagaataccg ttatctactc ttgggtatat cggaaaggat ttggccaaac tcaaatctga
    10561 ccacggataa acttggagac gagttcgcct ttggagttga cgtgcagttt tttgtagata
    10621 tttttgatgt ggaaacggat gg
//
LOCUS       DAOQNQ010000091        10623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2239_length_10623_cov_4.356264, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000091 DAOQNQ010000000
VERSION     DAOQNQ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10623
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2239_length_10623_cov_4.356264"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3047)
                     /locus_tag="PK754_07340"
     CDS             complement(<1..3047)
                     /locus_tag="PK754_07340"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNB09355.1"
                     /translation="MKRFLLLISILIITAATQIHAQGPVAWYMFNGNARDTSGNGKHG
                     LEENMSYAADRFGNTNEAAYFSGLAQISANYKGFPTGNSPRTISLWVNKSGNTAKPPM
                     LVGWGYRGSNLMSSLTLQVHTTPYYWGYADDLSSAGVTVVDSQWYHLVFTYEGGVGKF
                     YINGQLNSSGALSPNTPDTLLWIGNFQTQEEFGNFKGKLDDILIYNRALLPNEIDSLY
                     HDGIPTITSFTPTSGPIGTSVTITGTNFNTTAANNVVYFGGVKASVTNATSASLTVTV
                     PTGATYAPITVTDTTTRLSVASKLPFIVTFSGIRGVTSGSFASKVDFVTGTNPNPLSI
                     GDMDGDSKLDIVAINGGENSVSVFRNTSTTGTISTGSLASRVNFNTGNTPFEVAVGDL
                     DGDGLFDMAIVNPNSGTISLLRNTSTTGSITSGSFAAKVDLMAGNFPNGVAIGDIDGD
                     GKADLAVTNQNSNTVSIFRNISVKGSLSTSSFQTKLDFVTGSTPYGIAIYDLDMDGKR
                     DILFANSGDNSISICRNISTIGNITTGSFLSPINFATGANPQQISVSDIDGDEKPEII
                     VSHFGGSTISLFQNTSSIGSITSESFAPKIEFTTGTGPYEISIADIDGDSKPDIAVTN
                     FNSNTVSILRNTSSVGSITASTLASKIDFSTGNSPNGVILGDIDGDGRVDVAVSNYGA
                     STISLLKNTTNDLVAYYPFTGGSTADSSGNGNNGTNNGATPTTDRFGNANGAYSFNGT
                     SNYVSTNLDVQPSAIPITSWSAWIYPTRLNYASNQAILSSDDGLADRSLTIRQTTDSI
                     AVYVGGGVEWRPFKLNANQWQHLVVTYKSDNIYVYLNGVKYTYGSAGNILTTVNKLTV
                     GKNPGLSNYFQGNIDDIVIFNRQLSDQEVDSLYHVGNWNSPPVVTSALRDTSLNEDFG
                     KVFIRRLNTVFTDVETSSLSDTAYVLSGSVTALIPSGTDSLYLQSVANYSGTASIRIK
                     ATDGSNASVSDTFTVTINAVNDAPIRTAMIPDTVLGEDFDKTFR"
     gene            complement(3220..4545)
                     /locus_tag="PK754_07345"
     CDS             complement(3220..4545)
                     /locus_tag="PK754_07345"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNB09356.1"
                     /translation="MKLTLGLIALWLSMEGTGALAQIQAKKTTVAVLDFQSSGGISTD
                     EVKTLCNRFRGLLVKTSAFDVLEREKMGEILKAQDFNMSDACNTQACAVEVGKLLGVE
                     AMIAGDIGKIGETYTIDLRMIDVGTSKILSTQTRDFQGKIDGLLATMTSIAASFAKVD
                     KDAVVEVGDIYIASTPSGAEILINGKPVGDVTPKLMENMTAGSYHIELRLGDLRARKA
                     VEIKGATIANIEMTLELPKGRVRLLSSPMQAIVRVDTTMAGQTPLLLDLPIGRHILTF
                     SKPGYAEEAVETDVVENQQQKNISVQMHRVYSVTIMPVIPLSVRMSPVLVSVDGKPVG
                     QNTVTVPLTEGEHSVVVSGAAIKEQTRTISVSGPLSVSMDVEPLVSGMATEYKPVVPT
                     PTKRFLGLSQRQWVIIGGVAVTSGVTALLLRGGKKPSPAPIEFPEPPND"
     gene            complement(4771..5181)
                     /locus_tag="PK754_07350"
     CDS             complement(4771..5181)
                     /locus_tag="PK754_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09357.1"
                     /translation="MKFFKIGSYILLSTTFIHLLGHFQERIPANEAEKQLFELAQNYK
                     FEIMGSTRSLMDFQNGFSLFFALFCLFVAVLNLFIASRMTDRIEVMRGVLKINIAGMA
                     IATGICIVYFFAAPLTFMTLPLVFYVIAFLRLGR"
     gene            complement(5235..6188)
                     /locus_tag="PK754_07355"
     CDS             complement(5235..6188)
                     /locus_tag="PK754_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HNB09358.1"
                     /translation="MLFSVATLWSQILPENRLVHTYSIVARDPETGDMGVAVQSNWFS
                     VGSLVTWAEAGVGAIATQSFVNPSFGPRGLELLKQGKTAQEALKILIDSDEGRAVRQL
                     SIVDAKGNVAAHTGEKCIPAAGHALGEQFSVQANLMLNDKVWPAMHKAFKEAQGPLAE
                     RMVAALEAGQSVGGDIRGKQSAAILVVKAKATGKIWEDRMIELRVEDHAEPVLEIRRL
                     LKMYRAYELMNKGDLAIEHHEMDKAMALYGGAEKMFPDNLEMQYWHAVALANAGKMSD
                     ALPMFKKIFAKDNNWRILTERLPDVGLLTVKPEELKSILLQ"
     gene            6478..8523
                     /locus_tag="PK754_07360"
     CDS             6478..8523
                     /locus_tag="PK754_07360"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HNB09359.1"
                     /translation="MRTLRFFMHALLCITALSYFACADKAPMIKGKTIEAENLDTTVA
                     PCNDFYQYANGGWLKNNPIPAAFSRWGSFNELADFYTTALRGLLETAAKDKNAQKGSN
                     QQKIGDFYYTGMDSAGIEAAGLKPLASEFARIDGIKDVKDLAVVLSYYHQFAMDPAFN
                     VFVGQDDKNSTMMIANAYQGGLGLPDRDYYTKTDEKSKTIRSAYEKYIAKLLMLSGID
                     EKSSVKAASAIMGIETQLAKASMTRVEQRDPNAVYHKMTVEELEKTAPNFMWKTYFES
                     LGLAAMKDLNVGQPHFFKELSGMIKSVSIADWKSYLRFHVLNYAAPYMSSDFVNASFE
                     FQGKTLTGQKEMQPRWKRVSNVTDQSLGDALGQVYVEKYFPPASKKKALAMVNNVKEA
                     FRDRLMKLSWMSDSTRQKALAKLNTFVVKIGYPDKWKDYSTLEIDRQSYLGNVMRAQQ
                     FESKRLLAQVGQPVDRNRWGMTPPTVNAYYNTGMNEIVFPAGILQYPFFNPDADDAFN
                     YGSMGAVIGHEITHGFDDQGSQYDAEGNLKNWWSQEDRKQFESRADRVVKQFDGFVAI
                     DSMHVNGKLTLGENIADLGGLSIAYDAMIKAQNGKTPPKIGGFTAEQRFFLAWAQIWR
                     TNIRDERLRLMINTDPHSPGKFRVNGPLENMPEFYKAFGCKESDGMVKTDSNRAQIW"
     gene            8639..9214
                     /locus_tag="PK754_07365"
     CDS             8639..9214
                     /locus_tag="PK754_07365"
                     /inference="COORDINATES: protein motif:HMM:NF019106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNB09360.1"
                     /translation="MEQHHDTIVGEIWKQLRENPTYFISNMGRVKSYKYDQINGRILK
                     PRISGKNHLVVSLRRNNIKQSVYIHHLVAQYFLPPPAPDQKLVFHINGDYNNNQATNL
                     MWGTHKDHLLRSSQKNPRVHYYFFSNRVSRYDTGKITAADAEDIRKMLKNGLTGRRIA
                     KMYGISEMQVTRIRRFENWNPDKKNAQPLAS"
     gene            9343..>10623
                     /locus_tag="PK754_07370"
     CDS             9343..>10623
                     /locus_tag="PK754_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNB09361.1"
                     /translation="MIDIPFEKHILDNGLTVILHEDHKAPVVAVNVWYHVGSKNEKPG
                     KTGFAHLFEHLMFNGSEHMDVDYFQVMENIGATNLNGTTNEDRTNYFQNVPVSALDIA
                     LWMESDRMGHMVGAITQAKLDEQRGVVQNEKRQGENEPYAVEEELIVQATWPVGHPYS
                     WTVIGSMEDLDHASLEDVHAWFRNYYGAANAVLVIAGAIDPKDALTRAKKYFGEIPSG
                     PPVSRYKSWIARRSGRQRAETHDRVPLAKITKVWNIPEWGTTEAHHLDLISDILGSGK
                     NSRLYKRLVYDEMLATDVNAYVDLREIAGQFTITAKARPGIDIAQLEKAIDEELVKLC
                     TEGPSEKELTRVRTQFFAGFVRGIERIGGFGGKSDILARGEIFSSNPQAYKKNMHDVE
                     SATTEKLRDTARHWLSDGDYTLTTKPFPEFQITDA"
BASE COUNT         2996 a         2551 c         2287 g         2789 t
ORIGIN      
        1 cgaaatgtct tatcaaaatc ttcacccagc accgtatccg gtatcatcgc tgtgcgtatg
       61 ggcgcgtcat tcacggcatt gatcgtaacc gtgaaggtat ccgataccga tgcattcgat
      121 ccatccgtcg ccttgattcg tatgctggct gtgccgctat agttcgcaac gctctgcaaa
      181 tataaactat ccgtacccga aggaatcagc gccgtcaccg aaccggataa cacatacgct
      241 gtgtcactca gactggaagt ttctacatct gtaaacaccg tattgagtct gcgtatgaat
      301 actttcccaa agtcttcatt aagcgaagtg tctcgcaatg cagatgtaac taccggagga
      361 ctattccaat ttccaacatg ataaagcgaa tcaacttctt ggtcggaaag ttgacgattg
      421 aagatcacaa tgtcatcaat attaccctga aaataattgg ataaacccgg attttttcca
      481 acggttaact tgttaacggt agtaagaata tttccggcag aaccataagt gtacttcaca
      541 ccattgagat aaacataaat attgtctgat ttataggtga cgaccaaatg ttgccattga
      601 tttgcattca gtttgaatgg tcgccactca acaccaccac ccacataaac tgctatggaa
      661 tccgttgttt gtctgatcgt taacgatcga tccgctaatc cgtcatcact gctcaggatt
      721 gcctgattac ttgcatagtt cagtcttgta gggtaaatcc atgccgacca agatgtaatc
      781 ggaatcgcgg acggttgaac atctaaattg gtcgaaacat aattacttgt tccattaaaa
      841 ctatacgccc catttgcatt cccgaatcta tccgtcgtcg gcgtcgcacc gttattggta
      901 ccattatttc catttccgct ggagtcagcg gtgctgccgc cggtgaaggg gtagtaagca
      961 acgaggtcgt ttgtagtgtt tttcaataga gaaattgtac ttgccccata atttgacact
     1021 gcaacatcta ctctaccatc gccatctata tcaccaagaa taactccatt agggctgttt
     1081 ccagttgaaa aatcaatctt tgatgcaaga gtgctcgcag taatgcttcc aaccgaactt
     1141 gtattccgta aaatcgaaac agtgttgcta ttaaaattcg taacggctat gtcaggcttt
     1201 gagtcaccat caatatcagc aattgaaatt tcgtatgggc ccgtaccagt agtgaattca
     1261 atcttaggcg caaaagactc tgaagttata cttccgatag aacttgtgtt ttgaaaaaga
     1321 gatatcgtac tacctccaaa atgagagaca attatttccg gcttctcatc gccgtcaata
     1381 tcgcttacgg aaatttgttg cggatttgca cctgtagcga agtttattgg agataaaaac
     1441 gaacctgtag tgatatttcc tatcgtacta atgtttcgac aaatagaaat cgaattatcc
     1501 ccagaatttg caaatagtat gtcgcgtttt ccgtccatat ccagatcata tattgcaatg
     1561 ccatagggag tactgccggt gacaaaatcc agttttgttt gaaatgaaga tgttgaaagg
     1621 cttcctttga cgcttatatt tctaaaaatt gaaactgtat tgctgttttg atttgtgacg
     1681 gccaaatctg ccttcccatc tccatctata tctcctatgg caactccatt cggaaaattc
     1741 ccagccatca aatctacttt agctgcaaaa gaacccgatg taatactacc agttgtactt
     1801 gtatttcgta gcaacgaaat cgttccactg tttgggttta caattgccat atcgaataag
     1861 ccatctccgt ctaaatctcc aacagccacc tcaaatggag tatttccagt attgaaattt
     1921 actctcgaag ctaatgatcc ggttgaaata gtacccgtag tacttgtatt tcgaaatact
     1981 gaaacactat tttcacctcc atttatagct acaatatcaa gtttgctatc gccgtccata
     2041 tcaccgatag acaatggatt tgggtttgtt ccagttacaa aatctacttt tgatgcaaat
     2101 gaacccgatg ttacgcctcg aattccagaa aatgttacaa taaaaggtaa cttggatgcc
     2161 acgctcaatc tcgtcgtcgt atccgtcacc gtgataggcg catatgtcgc gccggtggga
     2221 acggtcaccg ttaggctcgc ggaagtcgca ttagtcacac tcgcttttac tccgccgaag
     2281 taaacgacat tgttagccgc ggtcgtattg aaattcgtcc ctgtgatcgt gacgcttgtg
     2341 ccgatagggc cggaggttgg cgtgaaggag gtgatcgtgg gaataccatc gtgataaagt
     2401 gaatcaattt cattcggtaa tagggcgcga ttgtaaatca aaatatcgtc aagttttccc
     2461 ttgaaattgc caaattcctc ctgagtttga aaattaccta tccaaagcaa agtatcagga
     2521 gtattcgggc ttaatgctcc gctcgaattc aactgaccgt taatataaaa ttttcctact
     2581 ccgccttcat aagtaaaaac aagatggtac cactgtgaat caacgaccgt tacgccagca
     2641 gatgaaagat catcggcata tccccaataa tatggggttg tatgcacttg cagcgttaat
     2701 gaactcatca gattgcttcc acgatacccc catcccacga gcatcggagg tttagcggta
     2761 ttcccagatt tattgaccca cagactgatt gttcgcggcg agtttccggt aggaaatcct
     2821 ttataatttg ctgagatttg agcgagtccg ctaaagtaag ctgcttcatt agtattgcca
     2881 aatcggtctg cagcatagga catattctcc tcaagcccat gcttaccatt cccgctggta
     2941 tctctggcat tgccgttgaa catgtaccat gcgaccgggc cttgcgcgtg gatctgcgtt
     3001 gccgctgtga tgatcaggat gctgatgagt agtaaaaagc gtttcatata gttgatctcc
     3061 gaagtttacc ttcaggatgt tttgtgaatg atattatttc tgttttacaa ccactctccc
     3121 ctgacaagct tggaacattg ccttttttcc ataccttgtt aaagcgagga tcgtactatt
     3181 tgggaatcgt agaaatttaa tattgctatc attgttgtat caatcattcg gaggttccgg
     3241 gaattcgatc ggagcgggtg atggcttttt gccgccacgc agtagcaacg ccgtcacgcc
     3301 acttgtaacg gcgacaccac cgatgatcac ccactgccgt tgactaagac caagaaatct
     3361 ttttgtcggg gttggaacaa ccggtttgta ttcggtagcc atacccgata cgagcggttc
     3421 cacatccata ctcaccgaaa gcggcccact cacgctaatg gtacgtgttt gctctttgat
     3481 cgctgctccg ctgacgacta cgctatgctc gccttctgtc agcggtacgg tcactgtatt
     3541 ttgaccaacc ggttttccat ccaccgaaac taataccggc gacatgcgta ccgacagagg
     3601 tatcaccggc atgatcgtca cgctatacac gcggtgcatc tgaacactga tattcttttg
     3661 ttgctgattt tctacgacgt ccgtttccac tgcttcctct gcatatcccg gctttgaaaa
     3721 agtcagaata tgccgcccaa tcggaagatc aagtaaaaga ggcgtctgcc cggccatcgt
     3781 cgtatccaca cgcacaatcg cctgcatcgg gctagataac agtcgaaccc ggcctttagg
     3841 aagctccaaa gtcatctcta tatttgctat ggtagcccct tttatctcaa cggctttgcg
     3901 tgcccgcagg tcaccgaggc ggagctcaat atggtaactg cctgcggtca tgttttccat
     3961 taatttgggc gtaacgtcgc cgactggttt accattgata aggatttccg ccccggaagg
     4021 agtggacgct atatagatat ccccaacttc gacgaccgca tctttatcta ctttggcaaa
     4081 tgaagcagcg atcgaagtca tggttgccag aagcccgtca atcttgcctt ggaaatcacg
     4141 agtctgcgtg gacaaaattt ttgaagtccc aacatcaatc atgcgcagat caatggtata
     4201 agtttcccca attttcccaa tatccccggc gatcatcgct tcgaccccaa gcaatttgcc
     4261 tacttccacg gcacatgcct gggtattgca agcatccgac atgttaaagt cttgtgcttt
     4321 gagtatttca cccatttttt ctcgttcgag aacatcaaac gcactggtct tgaccaagag
     4381 ccctctaaac cgattgcata gcgtcttgac ctcgtcggtg gatataccgc cgctactctg
     4441 aaaatcaaga acagcaacgg ttgttttttt ggcttggatt tgcgccaaag ccccagtgcc
     4501 ttccatagaa agccacaaag cgatcagccc gagggttaac ttcatacgga gattcccggt
     4561 tttaaggtta aaaattatta aagattggaa gcacaaaccg acgcctatga atgagagacc
     4621 cgcatggttt gtaaacgaag taagagtaaa aaactaggcg ctaacaagct ttttatagtg
     4681 agtatgtaca atatgacact aatttcacag cgctaaaaat gatttatgtc atgaatttct
     4741 tagttgtcaa gcagacacaa taaaatttct ttacctccca agccttaaaa aggcaataac
     4801 atagaaaacg agcggcaagg tcataaatgt cagcggtgca gcaaaaaaat acacgatgca
     4861 tatacccgtg gctatcgcca taccggctat attgattttc aaaaccccgc gcatcacttc
     4921 tatccgatcg gtcatccgtg aagcgataaa aagatttaaa acggcgacaa acaaacaaaa
     4981 aagcgcgaaa aacaaactaa atccgttttg aaaatccatc agcgaacgtg tactgcccat
     5041 gatttcaaat ttataatttt gagccagttc aaatagttgt ttttcggctt cgttggccgg
     5101 tattcgttcc tgaaaatgcc cgagcaggtg aataaaggtc gtagacagta ggatatatga
     5161 acctattttg aagaatttca tacgactgaa cttcctttct atacttagaa acgattatcg
     5221 ccacagatca aaaactattg taaaagaata gacttcaatt cctccggttt gactgtcagc
     5281 aaacccacat ccggcaagcg ttccgtgaga atacgccaat tattatcctt ggcgaatatt
     5341 tttttgaaca tcggtagcgc atcgctcatt ttaccggcgt tggccagcgc gacagcatgc
     5401 cagtattgca tttcaaggtt atcgggaaac attttttcag cgccgccata cagagccatc
     5461 gccttatcca tttcgtgatg ctcgatagcc aaatcgcctt tattcatcag ttcgtaagcg
     5521 cgatacattt tgagaagtcg gcgtatttcg agaaccggct cggcatgatc ttcaacgcgt
     5581 aactcgatca tccggtcttc ccagattttc ccggtagctt tcgccttaac gacaagaatg
     5641 gccgcagact gtttaccgcg aatatcacct ccgacgcttt gcccggcttc cagtgcggca
     5701 accatccgtt cggcgagcgg cccctgcgct tccttaaaag ctttatgcat cgccggccat
     5761 actttgtcat tgagcatcag attcgcctgt actgaaaatt gttcccccaa cgcgtgaccg
     5821 gctgcgggaa tacatttttc gcccgtgtgg gcggcaacat ttcctttggc atctacgata
     5881 gataattggc gaacggcgcg accttcgtcg gaatcaatta atattttaag cgcttcctgc
     5941 gccgttttgc cttgcttcaa gagttccagt ccgcgcggcc cgaacgacgg attgacaaac
     6001 gattgtgtgg cgatggcgcc gacaccggcc tccgcccaag tcacgagcga tccgacggaa
     6061 aaccaattgg actgcaccgc gacgcccata tcaccggttt cgggatcgcg cgcgacgatc
     6121 gaatacgtgt gtacgaggcg attttcgggt aaaatctgcg accaaagggt tgcaacgcta
     6181 aaaagcatta accaccagtg tttcatggga tttccttcca aaaaaaattg agcgatgacg
     6241 gatgagatat cgaaatgccg atcaatatac cggatatgtt agtatttcgc aagcattgcg
     6301 cgatctcgtt tctttgttaa agaaaaatca acatacatcc gagcaggccc ccatccatat
     6361 ccgaagtcga aatttgcagc tcaaatttta tactataatt ttttgcattt atctcaggct
     6421 ttgcctacaa ttgctggcct aaaaaattac atccctaata atcggagtaa accctttatg
     6481 cgaacgttac gtttttttat gcatgcattg ttatgtataa ccgcgctgtc ttatttcgcc
     6541 tgcgccgata aagcgccgat gatcaaaggt aaaaccatcg aagcagaaaa tttggacacc
     6601 acggttgcac cgtgcaacga tttttatcag tacgccaacg gcggttggtt aaaaaacaat
     6661 cccattccgg cggccttcag ccgctggggc agtttcaatg aattagccga tttttataca
     6721 acggcgctgc gcgggttatt ggaaaccgca gcaaaggata aaaacgctca gaaaggttcc
     6781 aaccaacaaa aaatcggcga tttttattat accggtatgg atagcgccgg tattgaggct
     6841 gccgggttaa aaccactggc atccgaattt gcacgcattg acggcataaa agatgtcaaa
     6901 gatttagccg tcgttttatc atattaccat cagtttgcga tggatccggc gttcaatgtt
     6961 tttgtcggac aagacgataa aaacagtacc atgatgatcg caaacgcgta ccagggcggg
     7021 ctcggtttac ccgatcgcga ttattacacc aaaacggatg aaaaaagtaa aaccattcgt
     7081 tcggcctatg aaaaatacat cgccaaactt ttaatgttat ccggtataga cgaaaaatca
     7141 tccgtcaagg ccgcatcggc gatcatgggc atcgaaacgc aattagccaa agcctccatg
     7201 acccgcgtcg aacaacgtga tcctaatgcc gtgtaccata agatgacagt tgaggaattg
     7261 gaaaaaacgg ctcccaattt catgtggaaa acttattttg agagtcttgg tcttgccgca
     7321 atgaaagacc tcaacgtcgg ccaaccccat tttttcaaag aactcagcgg catgatcaaa
     7381 tcggtatcca tcgccgattg gaaatcttac ctacgttttc acgtactgaa ctatgcggcg
     7441 ccgtatatga gttcggattt tgtgaatgcc tcgtttgaat ttcagggaaa aacgctgacc
     7501 ggacaaaaag aaatgcaacc gcgctggaaa cgtgtcagca acgtcaccga tcaatcgctc
     7561 ggcgatgcgc tcggtcaggt gtatgttgaa aaatattttc ctccggcatc aaagaaaaaa
     7621 gccctcgcga tggttaacaa cgtaaaagaa gcattccgtg atcgcctgat gaaactaagc
     7681 tggatgagcg acagcacacg tcagaaagcg ctcgccaagc taaatacctt tgtcgtaaaa
     7741 atcggttacc cggacaaatg gaaagattat tcgactctcg aaatcgacag acaatcctat
     7801 ctcggcaacg tcatgcgcgc tcaacaattt gaatccaaac gattgctggc gcaagtcgga
     7861 caacccgtgg atcgtaatcg ctggggcatg actccgccga ccgtcaatgc gtactacaat
     7921 acgggcatga atgaaatcgt ttttcccgcc ggtattttgc aatatccatt ttttaatccc
     7981 gatgccgacg atgcatttaa ctacggttcg atgggcgccg tgatcggtca tgagatcaca
     8041 cacgggtttg atgatcaagg cagccagtat gacgctgaag gcaacctcaa aaactggtgg
     8101 tcccaagaag accgcaaaca attcgaatcc cgtgctgacc gcgttgtgaa acaatttgac
     8161 ggttttgtcg cgatcgactc catgcatgta aacggtaaac tcacgctggg agaaaatatc
     8221 gccgacctcg gcgggttgtc catcgcttac gatgccatga tcaaagcaca aaacggaaaa
     8281 acgccgccta aaatcggcgg attcactgcg gaacaacgtt tcttcctcgc ttgggcacaa
     8341 atctggcgga ccaacatacg cgatgaacgc ctgcgtctga tgatcaacac cgatccgcac
     8401 tcgcccggaa aattccgcgt caacggcccg ctcgaaaaca tgcctgaatt ctacaaagct
     8461 ttcggatgca aagaaagcga cggtatggta aaaaccgaca gcaaccgcgc acaaatttgg
     8521 taaataaaaa atataacaag aggtgagtaa ctttattcac ctcttgttat ctatatagat
     8581 ttttattagt atccgatacc gtctaataat ttttataaac gaaaggtcat aaatcctgat
     8641 ggaacaacac cacgatacta tcgtggggga gatttggaaa caattgaggg aaaacccgac
     8701 gtattttatc agtaacatgg gtcgggtaaa aagttataaa tacgatcaaa tcaacgggcg
     8761 tatactgaaa ccacgtatct ccggtaagaa tcatttggta gttagcttac gtaggaacaa
     8821 tatcaaacag tccgtttaca ttcatcatct cgtcgcgcaa tactttttgc cgccgcctgc
     8881 acccgatcaa aagttagttt ttcacatcaa cggcgattat aataacaatc aagcaaccaa
     8941 cctgatgtgg gggacgcaca aagatcattt attacgttct tcacagaaaa acccgcgcgt
     9001 acattattat tttttctcaa atcgggtttc gcgttacgat actggcaaaa taacagccgc
     9061 cgatgccgaa gatattcgca aaatgcttaa aaacggctta accggacgcc gtattgccaa
     9121 aatgtacggt atcagtgaga tgcaagtaac gcgcattcgt cgttttgaaa attggaaccc
     9181 cgataaaaag aacgcacaac ctttggcttc gtgagattat atcttaatac gttaaaaagc
     9241 gcggtcgcat ccgcgctttt tttttgcgtt gaaaatatga tcccgtttag agatactcat
     9301 tcaccattta tttgaaaaac taatacgcga gcgataaaat ccatgattga tattcctttt
     9361 gaaaaacaca ttctggataa cggattgacc gtaattttac atgaagatca caaggcgccc
     9421 gttgtcgcgg tgaatgtgtg gtatcacgtc ggatccaaaa acgaaaaacc ggggaaaaca
     9481 ggttttgcgc atctttttga acatctgatg ttcaacggca gcgagcacat ggatgtggat
     9541 tatttccaag tcatggaaaa tatcggcgcc acgaatctga atggtaccac caacgaagat
     9601 cgcaccaatt acttccaaaa cgtacctgta tccgcattgg atatagcgtt atggatggag
     9661 tccgatcgta tgggccacat ggtcggcgcg atcacgcaag ccaagcttga cgaacagcgc
     9721 ggtgtggtac aaaatgaaaa acgtcagggc gaaaatgaac cgtatgccgt cgaagaagaa
     9781 ttgatcgtgc aagccacatg gccggtcggt catccctatt catggaccgt cataggttct
     9841 atggaagatc tggaccacgc atcgctcgag gatgttcacg cttggtttcg caattattac
     9901 ggtgcagcca atgcggtgct tgtcatcgcc ggcgccatag atcccaaaga tgctttgacg
     9961 cgcgctaaaa agtattttgg cgaaataccc tccgggcctc ctgtttcgcg atacaaatca
    10021 tggatcgccc gtcgcagcgg gcgccaacgc gctgaaaccc atgatcgtgt gccgctcgcc
    10081 aaaatcacca aagtatggaa tattccggaa tggggcacga ccgaagccca tcatctggat
    10141 ctgatcagcg acatcctcgg cagcggaaaa aattcacgtt tgtataaacg tttggtatat
    10201 gatgaaatgc tggccaccga tgtcaatgcg tacgtagatt tgcgtgaaat cgcaggccaa
    10261 tttaccatca cggccaaagc acgccccggg atagacatcg ctcaattgga aaaggctatt
    10321 gatgaagaat tagtcaaact atgcacggag ggcccttccg aaaaagaact cacacgcgta
    10381 cgcacacaat tttttgcagg atttgtgcgc ggtatcgaac gtatcggcgg atttggcggc
    10441 aaatcggata tattggcgcg cggagaaatt tttagcagta accctcaagc gtacaaaaaa
    10501 aacatgcacg atgtcgaatc cgctacaacg gaaaagctgc gtgatacggc tcggcattgg
    10561 ctaagcgacg gcgattatac tttgacaacc aaaccgttcc cggaattcca aataaccgac
    10621 gcc
//
LOCUS       DAOQNQ010000092        10619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2241_length_10619_cov_6.310678, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000092 DAOQNQ010000000
VERSION     DAOQNQ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10619
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2241_length_10619_cov_6.310678"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PK754_07375"
     CDS             complement(<1..424)
                     /locus_tag="PK754_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09362.1"
                     /translation="MISDSLARSLNQEQHLDWHASVTIRIAILDMNNGLRNLGIRNIK
                     RIIDNFTYYVRVNGYAVDFQVDHFHVRDRNEVPENGYDIYISSGGPGSPLEETTTDWE
                     EKFSKLLDQILEHNLSHENKQYFFGICHSFQLMAKKFEV"
     gene            complement(429..1232)
                     /locus_tag="PK754_07380"
     CDS             complement(429..1232)
                     /locus_tag="PK754_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNB09363.1"
                     /translation="MEETTSHTTEAGPAGPPQSSPNGRSFFRSNGLILALVAVSIALD
                     FFPFTGMILLPFEFFTTFIHESGHAIASLIMGESVSRIVINPDTSGYMQHTVSGGRLA
                     QGFIASAGYLGAALFAGILIIMSGRRNLARVILAVIGIGFAVAIVFYVRDFFTLAVSG
                     GLCAVLLLLAFKGGTWLNYIVINFLAVQCALNAVSDALTLVRLSMGATRSRYSLGHSD
                     ADTVAQLFFLPAIVWSILWVIIAVAILYYALKISARFQASAKPPQPSAG"
     gene            complement(1349..1732)
                     /locus_tag="PK754_07385"
     CDS             complement(1349..1732)
                     /locus_tag="PK754_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09364.1"
                     /translation="MKNHTLLIVMIVLMCIACKHTGYLIAADPALKNIEGFWSDTQRK
                     TCWIFEKNGAFQYGRQDSVVHVFMCGQYHISSDELVMTDRLGNRLVYDFFGQADTLWI
                     RSGDEAIRLLRSRPMRIINACPDDQ"
     gene            complement(1729..2391)
                     /gene="hxpB"
                     /locus_tag="PK754_07390"
     CDS             complement(1729..2391)
                     /gene="hxpB"
                     /locus_tag="PK754_07390"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016350848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HNB09365.1"
                     /translation="MIKSVIFDMDGLLINSEPFWRQAEREIFADLGIALTDADCERTM
                     GWRFDEVVHYWYKRKPWSGVDLYTVEQRVVDRVIALSTTHGKVMPGVYHALDFFTKHV
                     GKLALASSSAMRIIQAVLTHLKIKDRFEVIHSAEYEPYGKPHPGIFITTAERLNVNPT
                     ECLVIEDSLSGVIAAKAARMLAACVPDTAAAGKPQWCIADIQLASLDAWDDKTWAKIN
                     AL"
     gene            complement(2403..3071)
                     /locus_tag="PK754_07395"
     CDS             complement(2403..3071)
                     /locus_tag="PK754_07395"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNB09366.1"
                     /translation="MTPDEVITLLCQAHENEARQPLAHVPPDLIQSRIKLALLKKIQK
                     KISNDAALLENLWNTNIPEAQILVAMTSHHTSPDINLLQHRIETVQHPHVADALIESI
                     TPDLTDRKQWIDRWTSSPKEMVRRCGYTILALSGPAISEADCMAYLWNIVRDIHESPN
                     FAREAMNKAVIAIGQTSPRLNKEALFAVTQIGPVVLDHTDAPDALKILTDKKLQDKFK
                     KSRL"
     gene            complement(3117..6188)
                     /locus_tag="PK754_07400"
     CDS             complement(3117..6188)
                     /locus_tag="PK754_07400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB09367.1"
                     /translation="MKEGLPQSTVTSIVQTPDGYLWIGTFEGLVRFDGIQFEVFNKTT
                     EPVFISHSIISLTVMPDSSLWIGTGGGGIVIHKNNRFYSTASFSPLSERFVNAITMDH
                     DKRIWIATDRGLYHTNGDSLSRVFLPYNSSESIIHALQCDLNGNIWVGSHDGLYMINS
                     ISKKIIIVDSINKNISNKIAIHALSINKDNRILVGTNNGFFGVEQTDQSYGLKSYSEF
                     DIPNNTQIYAIRYDRHGNLWLGSSLGLFRWNQKHMELISAKEGLMNNTIISLYEDREG
                     IFWFGSESGGLSCFKDALFITYGNEEGILDNVIQCLFVDRKNRVWLGTDLGVSMLDFG
                     NQNTRHTSLHLGTNGLPNFPIHALYEDPEGAMWIGTDGGGLTRWSDGKYKLYNKTNGL
                     TNNFINVVLKDRSGIVWIGTKGGLHALHHNNISVYTTFNGLNHDIVRALHEDRKGGLW
                     IGTGSGVNILRNGKFESFKSPDETVSHSVMTFYEEESGDMWIGTHGGGIIRYHDGHFK
                     RYSSRDGLFDDVVFSILEDDSANFWMSCNRGIFRINKHQFERLDRRIITKLESYAYGI
                     DDGMKTNECNGGAQYGGIKDKSGRLWFATINGVSVVDPSKIVKNFDPPYVVITRFADA
                     QQNIRLDSTIFIPAGHDRIEFYYTGLSYSAPGKIRFKFKLDGYDNDWIDAGYRRTAVY
                     TNIPPGTYRFQVIAANASGIWNTQGASLDFVQQPFFYQTWWFVLLCLTGVVFVVLGAY
                     RYNVRQLIAREQQLAALVQMRTRKLEEEKERTENALQMAESARRVAEREREIAQQASE
                     TKSQLLGIAAHDLRNPLQGIVGYTDLVRVELNNPEKANKDLDQIQRSSSQMLHLINGL
                     LETMSIESGKLKLNKEKTDVSEITALVIQDNLIHAEKKAQRIHFHPHADSYVWGDAIR
                     LRESFENIVNNAIKYSPRDSEIDIKVEATDTIIRFSVRDRGPGLTLEDRRKLFGKFQR
                     LSAKPTGRESSTGLGLAIVKQLVELHDGQILVESTPGEGAVFIIELPRYNG"
     gene            6459..7802
                     /locus_tag="PK754_07405"
     CDS             6459..7802
                     /locus_tag="PK754_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNB09368.1"
                     /translation="MSLFEIDKSGFEQHTARVPLAERIRPRTLSMVIGQEHLLGHGKA
                     LRTQIEQGNVVSMIFWGPPGTGKTTLARVIAAETRSKFVSLSAVEAGVADVRRVIQDA
                     QALKRQTGERMMLFIDEIHRFNKSQQDALLHSVEEGTITLIGATTENPSFEVINALQS
                     RCKIYKLESLTDAHLEKILDQALKTDDLLKTLTVEIADLHSLIFYAAGDARNLLNTLE
                     LAIQITRPDTNGKHIVTREIVEEAYQQRNIHYDRAGEYHYDTISAFIKSVRGSDPDAA
                     LYWMARMLEGGEDPKFIARRLIVLASEDIGNADPYALTLAVSTFTAVDYIGMPEARIV
                     LAQCVTYLASCPKSNASYLAVDAAIHDVRSEKSHPVPLHLRNAPTKLMEKMDYSKGYK
                     YAHDFSDQGEPHFVEQIYLPDDLKARIYYQPTANGGEKRLKDRLEKIWKKRNKKD"
     gene            7806..8237
                     /gene="dut"
                     /locus_tag="PK754_07410"
     CDS             7806..8237
                     /gene="dut"
                     /locus_tag="PK754_07410"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNB09369.1"
                     /translation="MPVVKIQKLHPDAKLPSYAHPGDAGLDVCSVERVEILPHASALI
                     KTGFCMQLPDGTEAQVRPRSGLALKHQITVLNSPGTIDHGYRGEVGIILINHGSTPFI
                     VEPGMKIAQMVIAEVLHAHVEEATELTDSSRGQGGFGSTGR"
     gene            8240..8935
                     /gene="lipB"
                     /locus_tag="PK754_07415"
     CDS             8240..8935
                     /gene="lipB"
                     /locus_tag="PK754_07415"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNB09370.1"
                     /translation="MSNLFQIHRLGLVDYQVAWDLQKQLFDDCLSGRNGDAIIICEHP
                     HTYTVGKNGVESVGKHLLLNQEQLRENGILVFEIDRGGDITYHGPGQIVGYPILNLNR
                     YYRDVHRYLRDIEEVIIRTIRHYNIVGKRIEKVTGVWVDTPRGPEKICAIGVKVTRWV
                     TMHGFALNVNTNLDFFNGIVPCGITDKGVTSIAKLLNKEQDIREIENVIIHEFQSVFK
                     AAEMPIMENQGAI"
     gene            8932..9816
                     /locus_tag="PK754_07420"
     CDS             8932..9816
                     /locus_tag="PK754_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB09371.1"
                     /translation="MKKYIFCYVVIISEVLGRWGLAQNPDQAKSWYDKGRSAYLRFMS
                     DDYKTALSYYERSIAADSNYAPAFAGKAEALTLLALEKEQNGLAAEADFTHALRSAQQ
                     AIAKKPDQNLGYRALAQVYLNMDSKTYGEKAHDALQRAIELDSTDAESYFLLWLLTEN
                     DKPNSPLLRLSLKLDPNSFQTHYALGQYWSRNKQPDKAIDEYKLCTRINPDNYRSYYS
                     LGNVYSQIKRYDMAAEEYEKAIAINKNLYEAYFYVGVAYYYLDKNKKAKQRLSQYIEM
                     QPNPKYKLQAEQLLMDMK"
     gene            10293..>10619
                     /locus_tag="PK754_07425"
     rRNA            10293..>10619
                     /locus_tag="PK754_07425"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         3055 a         2257 c         2401 g         2906 t
ORIGIN      
        1 ctacttcaaa ttttttggcc atgagttgaa acgaatggca aataccgaaa aagtactgtt
       61 tgttttcgtg cgaaaggttg tgttctaata tttgatctaa aagttttgaa aacttttctt
      121 cccagtcggt tgtcgtctct tcgagagggc ttcccgggcc gccactggaa atataaatgt
      181 cgtatccgtt ttcgggcact tcattgcgat cgcgcacatg aaaatgatca acctgaaaat
      241 cgaccgcata accgttgacc cggacgtaat acgtaaaatt atcaataata cgtttgatat
      301 tgcgaatgcc gagattgcgc aggccgttat tcatatctaa aatagcaatg cgtatcgtga
      361 ccgaagcgtg ccagtccaga tgctgttcct gattgagaga gcgtgcgagt gaatctgaga
      421 tcatacgttt atccggcgga gggttgtggt ggtttggcag aagcttgaaa gcgcgcgctg
      481 atttttaatg cataatacaa aatcgctacg gcgataatga cccacagaat ggaccacact
      541 atggccggta aaaaaaacaa ttgcgccacg gtgtccgcat ccgaatggcc gagactatag
      601 cggcttcgtg tcgcccccat agacagacgt accagagtca atgcgtcgct gacggcatta
      661 agcgcacatt gaacggcgag aaaattaatt acaatatagt tgagccatgt accgcctttg
      721 aaagcgagta aaagcaagac ggcgcatagg cctccgctaa cagctaatgt aaaaaaatca
      781 cgtacataaa aaacaatggc tacggcaaac ccgataccga tcacggctaa aataactcgc
      841 gccagatttc gccggccact catgatgatc aaaatgcctg cgaataatgc tgcgcccaga
      901 taacccgcgc ttgcgatgaa accttgcgct aatcttccgc cggaaacggt gtgttgcata
      961 taaccgctgg tatcaggatt gatgacaatg cgactgaccg attcgcccat gatcaggctg
     1021 gcgatggcgt gaccgctttc gtgaatgaat gtggtaaaaa actcaaacgg cagaaggatc
     1081 atgccggtaa acgggaaaaa atctagtgcg atagataccg cgacaagagc taggattaaa
     1141 ccgttgctgc gaaaaaaact tctaccgtta ggtgaagatt ggggtggacc ggcggggccg
     1201 gcttcggttg tatgtgatgt ggtttcttcc aaaacacaga aacttacatt ttttttatat
     1261 ggcgtgcaaa tgaatgttga aaggaaacca tatttagaga ccggtggaaa caaatatata
     1321 gaaacacaat gatggataat ttgaacggct actgatcatc cgggcaagca ttaataatac
     1381 gcattggtcg tgatcggagc agtcgtatcg cttcgtcgcc cgagcgaatc cacaatgtgt
     1441 cagcttgacc aaaaaaatca tacaccaaac ggttacccaa tcggtctgtc atgacgagtt
     1501 catccgacga aatatgatat tgaccgcaca taaaaacgtg tacgaccgag tcttgcctac
     1561 catactgaaa agcgccgttt ttttcaaata tccagcatgt tttacgttgg gtatccgacc
     1621 aaaagccttc gatgtttttt aaagccggat ccgctgcgat caaatatccg gtatgtttac
     1681 aagcgataca catcaatact atcattacta tcagcagggt gtggtttttc ataaagcgtt
     1741 gatcttagcc catgttttgt cgtcccacgc gtctaatgaa gcaagttgaa tatcggcaat
     1801 acaccactgc ggtttgcccg ctgccgcggt atccggtacg catgcggcca acatacgggc
     1861 cgctttagca gcgatcacgc cgctaagtga atcttcgatg acgaggcatt ctgtcgggtt
     1921 gacgttgagc cgctccgctg tcgtgataaa aattcccgga tgcggtttac cgtagggttc
     1981 atattctgcg gagtggataa cttcaaaacg atctttgatt tttagatgag taagaacggc
     2041 ctggataatg cgcatcgcgg acgatgaagc taaagccaat ttgccgacgt gttttgtaaa
     2101 aaaatccaaa gcatgatata cgcccggcat aactttgccg tgcgttgtgg atagggctat
     2161 gacgcggtcc accacgcgct gctctacggt ataaagatca acaccgctcc atggtttacg
     2221 tttataccaa tagtgaacga cttcatcaaa ccgccaaccc atcgtgcgtt cgcagtccgc
     2281 atccgttaga gcgataccca aatcagcgaa tatttcgcgt tcggcttgcc tccaaaaggg
     2341 ttctgaattg atcaaaagcc cgtccatatc aaaaataacc gattttatca taacggagtt
     2401 tcctacaaac ggcttttttt aaatttatct tgcagttttt tatcggttaa tattttcaat
     2461 gcatcgggtg catccgtatg atcgagcacg accggcccga tttgtgtcac cgcaaaaagc
     2521 gcttctttat tgagccgtgg agatgtttgg ccgatagcga tgacagcttt attcatggct
     2581 tcacgagcaa aattgggcga ttcgtgaata tcacgtacga tattccacaa ataagccatg
     2641 caatcggctt cactaatggc tggtccggat agtgccaaaa tcgtataacc gcaccgacgc
     2701 accatttctt ttggggatga agtccagcga tcaatccatt gttttcgatc ggttaagtcg
     2761 ggagtgatgc tttcgattaa cgcatccgca acatgagggt gctgtacagt ctctatacgg
     2821 tgctgtagaa gattaatatc gggtgaagta tggtgtgagg tcattgcaac caaaatttgt
     2881 gcttcaggta tattggtgtt ccataagttt tccaacaaag ccgcgtcgtt agatattttc
     2941 ttttgaattt ttttgagtaa tgcgagcttg attcgcgatt ggatcaaatc ggggggtacg
     3001 tgcgcaagtg gttggcgcgc ctcgttttcg tgagcctggc ataacaaagt tataacttca
     3061 tccggggtca tgtggccttc ttaatcataa aattctatgt gatcagacgt ttgggattat
     3121 ccgttataac gaggcagttc gatgatgaaa acggcacctt caccgggtgt gctttcgact
     3181 aaaatttggc catcatgcaa ttcaacaagt tgttttacga tcgccaatcc aagaccggtg
     3241 gatgattcac ggccggttgg tttggccgaa aggcgctgaa atttaccgaa caattttcgt
     3301 ctatcttcta atgtaaggcc cggcccgcga tcacgtacag agaagcgaat aattgtatcc
     3361 gtagcttcta ccttgatatc tatttctgaa tcgcgcgggc tgtactttat cgcattattg
     3421 acaatatttt caaacgattc cctcaaacga atcgcatccc cccatacata cgaatcagca
     3481 tgcggatgaa agtgaatgcg ttgcgctttt ttttcggcat ggatcaggtt gtcttgtata
     3541 accaaagcgg ttatttctga aacgtcggtt ttttctttgt tcagtttaag cttaccggat
     3601 tcgatgctca tggtctcaag taatccgttt ataagatgaa gcatctgtga agacgatcgc
     3661 tgaatctgat caaggtcttt attggctttt tcgggattgt tcagttcgac gcgaaccaga
     3721 tcagtataac cgacaattcc ttgaagtggg ttgcgtagat cgtgtgcagc gattcccaat
     3781 aactgcgatt tggtttcgct tgcttgttga gcgatttccc gttccctttc ggcaacacgc
     3841 cgtgcggatt cggccatttg caatgcgttt tctgttcttt ctttttcttc ttcgagttta
     3901 cgcgtacgca tttgtaccaa agcggccagt tgttgttcgc gggcgatcag ctgacgtacg
     3961 ttgtatcggt atgcacccaa cacaacaaaa acaactccgg tcaaacacaa aagtacaaac
     4021 caccatgttt ggtaaaaaaa tggttgttgc acaaaatcca ggcttgcgcc ctgtgtattc
     4081 caaattccgc tggcattggc ggcaatgact tgaaaacggt acgtaccggg cgggatattg
     4141 gtataaacag ccgttctccg gtaacctgca tcaatccagt cattgtcata accatctaac
     4201 ttaaatttga aacgaatttt accgggtgca ctataactca aaccggtata ataaaattca
     4261 atgcgatcgt gccccgcagg aatgaatata gtcgaatcca atctgatatt ttgttgagcg
     4321 tcggcaaatc gagtaatgac aacgtaagga ggatcaaaat tttttacaat tttggaagga
     4381 tcaaccacag atacgccatt aatggtagca aaccacagcc ggccagattt atcttttatc
     4441 ccgccgtatt gcgctccgcc attgcattca ttggttttca tgccgtcatc aatcccgtaa
     4501 gcatagcttt ccagtttcgt tataatacga cggtctaatc gttcaaattg atgtttattg
     4561 attctgaaaa tgccgcgatt gcaactcatc caaaaattgg cactgtcatc ttctaatata
     4621 gaaaacacga cgtcatcaaa taatccgtca cgacttgagt agcgcttaaa atgcccgtca
     4681 tgataacgaa taatcccccc gccgtgtgta ccaatccaca tatcaccgct ttcttcttcg
     4741 taaaaagtca taaccgagtg gctcaccgtc tcgtccggcg acttaaaaga ttcaaattta
     4801 ccgttgcgga gaatattgac gccgctaccg gtgcctatcc agagccctcc cttgcgatct
     4861 tcgtgaagag cacgcacaat atcatgattc aaaccgttaa atgtcgtgta aacggaaata
     4921 ttattatgat gtaacgcgtg caggccgcct ttggtgccaa tccacacgat tcccgaacgg
     4981 tctttcaata caacgttgat aaagttattg gtcaaaccgt tagttttatt atataattta
     5041 tattttccgt cggaccatcg ggtcaatcca cccccgtctg ttccgatcca catcgcaccc
     5101 tcggggtctt catataatgc atgtatggga aagtttggta acccattggt tcccaaatga
     5161 agtgatgtat gccttgtgtt ttgattaccg aagtcaagca tgcttacacc aagatcggta
     5221 ccaagccata cccgattttt tcgatccaca aataaacatt ggattacgtt atccaaaatg
     5281 ccttcttcgt tgccatacgt aataaaaagc gcatctttga aacaacttaa gcccccggat
     5341 tccgaaccaa accaaaaaat accctcgcgg tcttcataaa gtgaaattat cgtattattc
     5401 atcaatcctt ctttggcgct gatgagttcc atgtgttttt gattccatcg gaataatcct
     5461 aatgaagatc cgagccatag attgccgtga cgatcatacc ggattgcata tatctgagtg
     5521 ttgtttggaa tatcaaattc ggaataactt tttaaaccat aagattgatc ggtttgttca
     5581 accccaaaaa aaccgttgtt tgttccgact aaaatgcgat tgtctttgtt tatggataaa
     5641 gcgtggatgg cgattttgtt tgagatattc ttgtttatgg aatccacaat aatgattttt
     5701 ttagaaatag agttaatcat gtataaccca tcgtgcgaac caacccaaat gtttccgttt
     5761 agatcacatt gaagtgcatg aatgatagat tcggatgaat tatacggtaa aaaaacgcgt
     5821 gataacgaat cgccattggt atggtacaag cctctgtctg ttgcaatcca aatacgttta
     5881 tcgtggtcca tggtaatggc gttgacaaac cgttcgctca aaggggaaaa cgaagcggta
     5941 ctataaaaac gattgttctt gtgtatgaca ataccgccgc cgcccgttcc aatccataac
     6001 gagctgtcgg gcattacggt taagcttata atgctgtggg atataaatac aggttcggtg
     6061 gttttattga atacttcaaa ctggattccg tcaaatctaa cgaggccttc gaatgttcct
     6121 atccataagt acccgtccgg agtttgtacg attgatgtta ccgttgactg gggtaaacct
     6181 tctttcaagt gccaattggt atgcgcgtat tgagtcaatc ggcgcgcagg atcaagagca
     6241 taagaaaaat ctaatcctac aaaaacacat acgaagaaaa aaagatgtac agatttaaca
     6301 agaagtgcat tcatatgtca aagggtgtga gggcaacaga actgaaaatt gcggttagca
     6361 agttaaacgc tgatccaaat tagaagtatt tctacaatta ttcaaatact tagaagtaat
     6421 tttttgcatt tgcatgtcga gcatgataga ttggtgccat gtcattattt gaaatagaca
     6481 aatcaggatt tgaacaacac acagcacgcg taccgcttgc agaacgcatc aggccgcgca
     6541 cgctaagtat ggttatcggg caagagcatt tgcttgggca cgggaaagct ttgcgtaccc
     6601 agatcgaaca aggtaatgtc gtatctatga ttttctgggg tccgccgggt accggcaaaa
     6661 ctactttggc gcgcgtgatc gccgcagaga cgcggtccaa gtttgtttcg ctgagtgccg
     6721 tggaggcggg tgtagcggat gtacgtcggg tcattcagga tgcccaggcg ctcaaacggc
     6781 agaccgggga acgaatgatg ttgtttattg atgaaattca ccggtttaac aagtcgcaac
     6841 aagatgcctt gctgcatagt gttgaagaag gaactatcac gttgatcggc gcgacgacag
     6901 agaacccttc gtttgaggtc atcaatgctt tacaatcccg atgtaaaata tacaagctgg
     6961 aatctttgac ggatgcacat cttgaaaaga ttttggatca agcgttaaaa acggatgacc
     7021 ttttaaaaac tctgactgtc gaaatcgccg atcttcacag tttgattttt tacgccgccg
     7081 gcgatgcccg taatttactc aacacgttgg aattagccat acagatcacc cgaccggata
     7141 cgaacgggaa acacattgtt actcgggaga tcgttgaaga ggcttatcag cagcgtaata
     7201 ttcattacga tcgggccggc gaatatcatt atgacacgat cagcgctttt attaaaagcg
     7261 tccgcgggag cgatccggat gcggcattgt attggatggc tcgtatgctc gaaggcggag
     7321 aagatcctaa gttcatcgct cgacgattga ttgttttggc gtcggaagat atcggcaacg
     7381 ccgatcccta cgcgcttacg ctggcggttt cgacgtttac agccgtagat tacataggca
     7441 tgccggaggc tcgcatcgtc cttgcgcagt gtgttacgta tctcgcctct tgtcctaaaa
     7501 gtaacgcatc ctatctggcc gttgacgccg ctatccatga tgtacgatca gaaaaatcac
     7561 atcctgtacc gttgcatctt cgtaatgcgc ctaccaaact gatggaaaaa atggattata
     7621 gtaaaggcta taaatatgcg catgattttt cagatcaggg ggagccgcac tttgttgaac
     7681 aaatatattt accggatgat ttgaaggcgc gcatttatta tcagcccaca gccaacggcg
     7741 gagaaaagcg gttaaaagat cggttggaaa aaatctggaa aaaacgaaat aaaaaggatt
     7801 gagttatgcc tgttgttaaa attcaaaaac ttcatccgga tgccaaactt ccttcgtatg
     7861 cgcaccccgg cgatgcaggt ttggatgttt gttcagtcga gagggtagaa atattacccc
     7921 atgcttcagc tttgatcaaa acaggttttt gcatgcagct tcctgacggc acagaagcac
     7981 aggtgcgacc tcgaagcggt cttgcactca aacatcaaat caccgtactc aattcacccg
     8041 gtacgattga tcacggttat cggggtgagg tgggtatcat tcttatcaat cacgggtcaa
     8101 ctccttttat agtagaaccc ggtatgaaaa tcgcacaaat ggtcatcgca gaagtgctgc
     8161 acgcacatgt ggaggaggcg acggagctca cggatagttc gcgcggtcag ggcgggtttg
     8221 gcagtacggg gcggtagata tgtcaaactt atttcaaata catcgtttgg gtttggtgga
     8281 ttatcaagtc gcttgggatc tacaaaaaca actttttgat gattgcctaa gtggccgaaa
     8341 tggtgatgcg attattattt gcgaacatcc tcatacatat acggttggta aaaacggtgt
     8401 tgagagtgtt ggaaaacatt tattgcttaa tcaggaacag ctccgggaaa atgggattct
     8461 ggtttttgaa attgatcgtg gcggcgatat cacatatcat ggtccgggac aaatcgtggg
     8521 gtatccgatt ctcaacctaa accgttatta ccgcgatgtc caccgatatt tgcgtgacat
     8581 cgaagaagta attattcgta cgatccgtca ttataatatt gtaggaaaac gaattgaaaa
     8641 ggttaccggg gtatgggttg atacgccgcg cggacctgaa aaaatctgtg cgatcggcgt
     8701 taaagtaacc cgatgggtca cgatgcacgg ctttgcattg aatgtaaata caaatctcga
     8761 tttttttaac ggaatcgtac cctgcggtat cacggacaag ggcgtaacat ccatagcaaa
     8821 attgctcaat aaagaacagg atatcagaga aatagaaaac gtgatcatcc atgaatttca
     8881 gtccgtattt aaagctgccg aaatgccaat aatggaaaat caaggagcga tatgaaaaaa
     8941 tatatctttt gttatgtggt aataatatct gaagtgttgg gtcggtgggg cctggctcaa
     9001 aatccggatc aagccaaatc ctggtatgat aaaggacgat ccgcgtatct tcgttttatg
     9061 tcagacgact acaagacggc attgtcttat tatgaaaggt cgattgctgc ggattccaac
     9121 tacgcaccgg cttttgccgg aaaagcggag gcgttgacgc ttttagcttt ggaaaaagaa
     9181 caaaatggtt tggcagcaga agctgatttt acccatgcgt tacgatcagc ccaacaagcg
     9241 atagctaaaa aaccagacca aaacttaggt taccgtgctc ttgcgcaagt atatctgaat
     9301 atggactcca aaacctacgg cgaaaaagcg catgatgcgt tacagcgtgc tatagagttg
     9361 gattcaaccg atgcggaaag ttatttttta ttgtggcttc tgaccgaaaa cgacaaacca
     9421 aacagcccgc ttttgcgatt atcattgaag ttagatccta attcgtttca aactcactat
     9481 gctttaggtc aatactggtc tcgtaataaa cagccggata aagcgataga cgagtacaag
     9541 ctatgtactc ggattaaccc ggacaattac cgctcttatt attcacttgg caatgtttac
     9601 tcacaaatca aacgatacga tatggccgcg gaagaatacg agaaagctat tgctataaat
     9661 aaaaacctgt acgaagcgta tttttatgtc ggtgttgcgt attattattt agataaaaac
     9721 aaaaaagcaa aacaacgact ctctcaatat atagagatgc aacctaatcc taaatataaa
     9781 ctgcaggctg agcagcttct catggatatg aaataaaacg ctccaatcgt gaaaacagaa
     9841 gaatgataat taattttatt aattttttga ttttcattca aatacctctt gacatcggaa
     9901 cctttttttt gtatcattgc aacccgctga tgaaatggcg aaaataagaa gcggcaggtg
     9961 ttaataaatt gttaattgtt cgccaaatgt tcatagaaat gagatttttg attctggtaa
    10021 ataagttttt aaaaaaactt attaaaaagt aaaaaaagac ttgcaatcag aaacaaattt
    10081 gtttatactt acgtcccgca gttgaaaaag cggtttgttc cttaaaaaac aagactatag
    10141 ctcgcgaaag cgaattataa attatattgc tgagaccttg agtcgaagcc caatgttgtt
    10201 tgaaaatgag agtatacgtc gatcaattca tttattgagt gattctggtc aagaataacc
    10261 atcttctttg ttaataacaa aaaaagatat tgacaacgaa gagtttgatc ctggctcagg
    10321 actaacgctg gcggcgtgct taacacatgc aagtctaacg agaaagcggt agcaatatcg
    10381 tgagtaaagt ggcgaacggg tgagtaacac gtaaccaatc tgccttgaag aggaggaaaa
    10441 ctgagggaaa ctttagctaa tcctccataa tgtggtgcct atcgcatgag aacatcatta
    10501 aagatttact cgcttcggga agaggttgcg tctgattagc tagttggcgg ggtaaaggcc
    10561 caccaaggcg acgatcagta gctggtctga gaggatgatc agccacactg ggactgaga
//
LOCUS       DAOQNQ010000093        10579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2249_length_10579_cov_5.666382, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000093 DAOQNQ010000000
VERSION     DAOQNQ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10579
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2249_length_10579_cov_5.666382"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..446)
                     /locus_tag="PK754_07430"
     CDS             complement(75..446)
                     /locus_tag="PK754_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB09372.1"
                     /translation="MKKIVIIDDDQDFVSVAGKVLSHIGKFDVVAHTDSSDAMDLIRR
                     EKPDLIIVDIMMPKVDGFTILRNVRTSADLQHIKVIVCSAKIFDVDKKNALKLGANEY
                     ISKITAASELVDKVNAVLGTV"
     gene            complement(443..1006)
                     /locus_tag="PK754_07435"
     CDS             complement(443..1006)
                     /locus_tag="PK754_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNB09373.1"
                     /translation="MIIHRYLRQKPAIPKSVFLATGAIIVGDVVMGEDCSVWFNAVIR
                     GDVNTIRIGSRTNIQDGAIIHCTLDKYATVIGDDVSIGHGAIVHGCTVHDHVLIGMGA
                     KILDRAVIHPYAMIAAGAVVREGFEVPERTLMAGVPAKPVRSLTDDEIAMIDRIPQNY
                     IRYAADYRTLNVLDSAVVFSSSQTSNV"
     gene            complement(1081..4017)
                     /locus_tag="PK754_07440"
     CDS             complement(1081..4017)
                     /locus_tag="PK754_07440"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNB09374.1"
                     /translation="MPRCITSSLFIALILTTASLRAQEETGYPYIKNYTMRDIPAGLQ
                     NWAVAQDQRGIMYFGNSNGLLIFDGIHWRVTETPGRAFIRSIAINTSDEVFAGAVDDL
                     GYLWPDSNGQRQFVSLLSAIPEPDRHFGDVWKSFCIGNRIVFQTFSRIFIFESAPPES
                     PERFRFVRTIQPPNSLHFSFQVRNAIYMIVRGHGLHLLQNDTLVLAPGGAFFASDRIY
                     AMLPWENDATMIFTRERGIFLWKDGKIAPFAGDVHTDLPRHQIYHATLLSDSTVAIAT
                     LDNGIWIMNRFGRRIAAFDRSSGLMDQSILYLFTDRENNLWAGLNQGIARVEYPSRYT
                     FFSERNGLTGSVFKIARHQGNLYVATFDGLYRLSKNNTHQLERVTGISSSVWHLYSAG
                     TSLVIAATDGVYALRDHVIQRISDRPYNYVIAPTLSDAKQILVGHAKGVDIYRQQNGR
                     WISTAAWQGIDDEIRTITEYAPGKFWLGTMFRGVMHYTTPGQVTRYGASHGLPPGEIR
                     PFMINERLLFATSSGIMTFDKDRFLRPPALQAPDDVFVGYGVWMHAVDRHNFWVSVET
                     KRGANTYRATMDDNGSIHYGKPILVRADRHVIWNGMQDDDGIAWFGGPDGIIRYDAHT
                     ADNTTAPFHALISRITLQSDTSRAEYNIRFRNPTQIPEYPAMGIRFECAATSFTRQEF
                     NEFQYWLEGFNHMWSDWTTETRRDYTNLPEGVFTFRVRARNFAGQIGKEDAFTFRVTA
                     PWHKSNWFMSLTAMVLVGFLIGAVRFATVRRWKRRVMDLELRERISRDLHDEIGANLS
                     NIALLSEMLSAKEKFENKEKEQLARISNVARRTVDTMRDIIWAIQPHQDQLDEMMLRM
                     KDLTSELLGDTLPYTLDFPSENPTRTVTMPYRQNIILIYKELLHNIIKHAQASFVHIR
                     LVITGDELVLVVTDNGIGFDAQHSHDGGYGVKNLKRRTRGIRGDIRWETAENGGTRTI
                     LRCTLA"
     gene            complement(4040..4912)
                     /locus_tag="PK754_07445"
     CDS             complement(4040..4912)
                     /locus_tag="PK754_07445"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNB09375.1"
                     /translation="MHLGYLFKESFSGLRRATLSTFVAVTIIAIALSLFSFFLIITLN
                     INQLVEDIRSRVELEAFLDRALDTPQSKKIEAAIRDIEGVESVTFISKSDAARILKKA
                     MGADDIFDLVESNPLPASFKIRLQSEYRNWQNVLAVAQSLETIDGVEEVSYQKELLLA
                     LDEKVDLYNMISIGLGCIAALAAVLLVSNTIKLSIYAKRDVIKTMKLVGAKNSFIAAP
                     FILEGILQGVLGSLIATGIAYALIRIVQRYLLPTLQFSWEIPAIVVGFGILLGLFGSF
                     VSIRFFLKEKISDM"
     gene            complement(5065..5208)
                     /locus_tag="PK754_07450"
     CDS             complement(5065..5208)
                     /locus_tag="PK754_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09376.1"
                     /translation="MNSSALAFMLITYIVVIGATGYFFYLALTTKPHDEPDSFTENDN
                     VPR"
     gene            complement(5205..6785)
                     /locus_tag="PK754_07455"
     CDS             complement(5205..6785)
                     /locus_tag="PK754_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HNB09377.1"
                     /translation="MSTNKEAWGSRVGLILAMAGNAVGLGNFLRFPVQAIQNGGGAFI
                     IPYLVCFLLMGLPLLFVEWAMGRYGGKNGHHSTPFILDSMDKRSFWKYIGVFGIFTNL
                     AVAAYYCYLESWTIAYVYHMLVGSFQGQNQDEVAGFFNHYLSFEGFENVIVWLLCLFL
                     NTWILSKGLSGGVEKVAKIGMPLLILFGILLAIRGFTLSAGENGAIYDGTVGLNFLWT
                     PQFDTIWDGKVWLAAAGQIFFTLSVGMGSIHCYASYVKSKDDIALNSMSAGWMNEFVE
                     VVLGSAILIPIAVGYLGIDKVIEMTASGGLGLGFKTLPYLFTQWGPILAALAGVFWFG
                     LLFFAGITSSLAMGTPCMGFMMDEFGWKREKAAWAFGFTILILGLPTVLFFHHGVFDE
                     YDYWAGTVSLVIFALFEIILFAWVFGMDKGWKEITDGADIKVPNVFKFIIKFITPILL
                     GWVFVKSLPDIWDTISNKKLYDKIAATTDTVQIAAIQESIMYVNASRFVLLGIFVGVS
                     ILVFIAYKKRIREGRFTS"
     gene            7174..8424
                     /locus_tag="PK754_07460"
     CDS             7174..8424
                     /locus_tag="PK754_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09378.1"
                     /translation="MVPFTKTFHRLRVIGLTSLIVVSVVLFGCKSDKDGDRVEDAKDE
                     CDNARGVAVDANGCPCVDSAALPEPLPIAAVRMYVDNSGSMRDFTNPTSLFRSTISEC
                     ISRIQKMKTDNIADTVRYHLIASSLLHTDYNILYKAIRTGDIMKAKSTPILSMIEQVQ
                     RRHEAQDISVLVSDFELSEKDADNLDLALKTDVLAKIAKHNAVGLYAFREKSVDRPYY
                     VMVIGTSASVLLFDREGGLRDYRHKIFLGVKYPVPEIAFLNYSQRDETTWFISQDFCR
                     TIEDASLAGKGYIQFAMGLDLKKYEGLQLDSVYLTKNTLIKDVDPQHVAMRWYTAHQF
                     KNLADEKEAEQLSTYSHVVVVKLHRLFENTNKYFFELAKAQDFERFYGSDPSMQALVH
                     GFREAYRGDTGPYFSVKLNIVKEQ"
     gene            8428..8814
                     /locus_tag="PK754_07465"
     CDS             8428..8814
                     /locus_tag="PK754_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09379.1"
                     /translation="MSDMFTALYEVWHGRQLEAVWRREIYPLAGFLMLLYGVFSAWLY
                     YFFLSLRIPKTAVDTRFFWSYNGLMAFLFGLIALGVFKWNTTINGLPGSAMLFMLANM
                     AYAFILSLVAGLAMKRYSTNASTIPF"
     gene            8827..10239
                     /locus_tag="PK754_07470"
     CDS             8827..10239
                     /locus_tag="PK754_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09380.1"
                     /translation="MSKLYIFGIGGTGARVIRSLTMLLASGVRIEADEVVPIIVDVDL
                     TNGNTVQSEETLKWYQKIRSRVQPESEFFTTPMRTIGQITGADPTAMFRFSFDSTHQK
                     KFRDFIDYSKLSAEDQFMVNLLYSEEYLDDELTEGFKGKPNMGSVALNTLTSMDEYDQ
                     FVNSFQPGDRIFVVSSIFGGTGAAGFPILLKNFCSQKSDPKLQKALKGALTVMPYFGV
                     QADNESQIDSNTFHAKTKAALAYYEKNLSGLHHLYYLADTIKELYPNFEGSTGQRNKA
                     HVIEVLGALAIIDFARQTFETSGAKTLCHEFALKEDGEVLTFTNLPDPAQALIKAPLI
                     RFLYLYYIMQFLLRSERKDAWIKNIFGTASALNDEDAKLLQKFLYMYADWITELAENK
                     RAFEPFYMQQFAESFAHALVKGYQLEPGGLFRTGLDDNFIISALGSNSRQLPATLTTM
                     GKLIKAGYAATDAVIQRFYK"
     gene            10264..>10579
                     /locus_tag="PK754_07475"
     CDS             10264..>10579
                     /locus_tag="PK754_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09381.1"
                     /translation="MGYLLNLKPARSVAQDHWTDTTVFHPNMINSIEDNQSDKVERPI
                     ASIPSPFAQMHLFDYAFRSVNGRGEMSGNSLYHRLISYTFDVMELLFHANNIGGVGGK
                     LEV"
BASE COUNT         2877 a         2461 c         2516 g         2725 t
ORIGIN      
        1 gggccgggcg tgggaatcga accgcgaacg ccccaaaatt tgtatttcat acgatatccc
       61 ttgtaaaaaa aagatcagac cgtaccgagt acggcattga ctttgtccac cagttccgaa
      121 gcggcggtga ttttggaaat atattcattg gcgccgagct tgagcgcatt tttcttatcc
      181 acatcaaaga ttttagctga acacacaatg actttgatat gttgtaagtc ggctgaggta
      241 cgaacattgc gaaggattgt aaaaccgtcc actttgggca tcatgatatc cacgataatg
      301 aggtcgggtt tctcacggcg aatcaaatcc atcgcgtccg aactgtcggt atgcgccaca
      361 acgtcaaatt taccgatgtg ggaaagaact ttaccggcga ccgaaacaaa gtcctgatcg
      421 tcgtcaatga taacgatttt tttcatacat ttgaggtttg tgatgaagaa aaaacaaccg
      481 ctgaatccaa aacgtttaat gttctgtaat ccgcggcata acgtatataa ttttgcggta
      541 tacgatcaat catcgcgatt tcgtcgtccg tcagagatcg tacaggtttg gcaggaacac
      601 cggccatcaa tgtgcgttcc ggtacttcga acccttctcg gacgacagca cccgccgcga
      661 tcatggcata cgggtgaatc acagcgcgat ccagtatttt agcgcccata ccgatcagga
      721 catgatcgtg aacggtacat ccgtgcacaa tggcgccatg accgatggat acatcatcac
      781 cgatcaccgt ggcatattta tccagcgtgc aatgaataat tgcaccgtcc tgtatattag
      841 ttcgtgaacc tatccgtatt gtattcacat cgccgcgaat cacggcatta aaccaaactg
      901 aacagtcttc acccataacg acgtcaccca caataatggc accggtcgct aaaaaaaccg
      961 atttcggaat agctggtttt tgacggaggt atcggtggat aatcatgctt agtttgataa
     1021 tgaatgtgcc gcaatataga gaccgagccg tggaatatca agcaaaaatt cccgtcccaa
     1081 ttacgccaaa gtgcatcgta atatggttcg tgttccgccg ttttcggctg tttcccaacg
     1141 gatgtcgccg cggattcccc gtgtccgccg cttcagattt tttacgccgt atccgccgtc
     1201 atgggaatgc tgggcgtcaa aaccgatgcc gttatcggta acaaccaaaa cgagttcatc
     1261 gccggtaata accaaacgga tgtgtacgaa cgaggcttgc gcatgtttga tgatgttgtg
     1321 aagaagctct ttgtagataa gtatgatgtt ttgccgatag ggcatcgtaa cggtacgggt
     1381 tgggttttcg gacggaaaat cgagtgtgta aggcaaggta tctcccaaca actcggaggt
     1441 caaatccttc atgcgcaaca tcatctcgtc aagttgatcc tgatgcggtt gaatagccca
     1501 aataatatcg cgcatcgtat ctacggttcg gcgtgcgaca ttgctgatgc gggccagttg
     1561 ctctttttct ttgttttcaa atttttcttt agcgctcagc atttctgaca gcaacgcaat
     1621 attactcaga ttggcgccga tctcgtcgtg cagatcgcgt gaaatgcgtt cacgcagttc
     1681 gagatccatc acacggcgtt tccatcgtcg cactgtggca aaacgtacgg ctccgatcaa
     1741 aaaaccaacg agcaccattg cggtcagact cataaaccaa ttgcttttgt gccatggtgc
     1801 cgtcactcta aaagtaaagg catcttcttt tccgatctga cctgcaaaat tgcgggcacg
     1861 cacgcgaaac gtaaagacac cttccggcag attggtataa tcccggcgcg tctcggttgt
     1921 ccaatccgac cacatatgat tgaaaccttc gagccaatat tgaaattcat tgaattcctg
     1981 acgggtaaaa ctcgtagcgg cacattcaaa ccgaatgccc attgccggat attccggtat
     2041 ctgtgtagga ttacggaacc ggatattgta ttcggctcgg ctggtgtcac tttgcaaagt
     2101 aattcggcta atcaaggcat gaaacggagc cgtcgtgttg tcggccgtat gggcgtcata
     2161 acgaatgata ccgtccggtc cgccgaacca ggctatgccg tcgtcgtcct gcataccatt
     2221 ccaaatcacg tggcgatcgg cgcggaccaa aatgggtttc ccatagtgga tggatccgtt
     2281 atcatccatc gtggcgcggt aggtattcgc gccgcgctta gtttctacgc taacccagaa
     2341 attatgacga tcaacggcat gcatccatac accataacct acaaagacat cgtcgggtgc
     2401 ttgcaatgcg ggagggcgta ggaaacgatc cttgtcaaac gtcataatgc cggatgaggt
     2461 tgcaaaaagt aagcgttcgt tgatcatgaa aggccgaatt tcaccgggcg gcaaaccatg
     2521 cgacgcaccg taacgcgtga cctgtcccgg cgttgtataa tgcatcaccc cgcgaaacat
     2581 cgtacctaac caaaattttc ccggcgcata ttctgtgatc gtacgtatct catcatcaat
     2641 gccttgccac gccgccgtcg aaatccatct cccgttctgt tgacgatata tatccactcc
     2701 tttggcatga ccgactagaa tttgtttggc atccgacagc gtgggggcga tcacataatt
     2761 ataaggccga tcgctgattc gctgaatgac atgatcccgc aaagcgtaca caccgtccgt
     2821 agcggcaata acaagggatg ttccggcaga gtacaagtgc catacggagg aagaaattcc
     2881 ggttacgcgc tccagttgat gcgtgttgtt tttagataag cggtaaaggc cgtcaaacgt
     2941 tgcgacatag agatttccct gatgacgggc gattttgaaa accgagccgg tcagaccgtt
     3001 acgttcggag aaaaaagtat aacgggaagg gtactcgaca cgtgcaattc cttgattgag
     3061 acctgcccat aaattatttt cacgatcggt aaaaagatat aaaatggatt gatccataag
     3121 accggatgat cgatcgaatg cggcaatgcg ccgaccgaaa cggttcataa tccatatccc
     3181 gttatccagc gtagcgatgg caaccgtcga atcgcttaat aatgtcgcgt ggtatatctg
     3241 atgccgcggc agatcggtat gaacgtcgcc cgcaaacggt gcgatctttc cgtccttcca
     3301 aagaaaaatg ccgcgttccc gagtaaaaat catcgttgca tcgttttccc acggcagcat
     3361 cgcgtaaatc cgatccgatg caaaaaacgc tccgcccggc gctagtacca gcgtatcgtt
     3421 ttgcaacagg tgtaatccgt ggcctcgtac gatcatatag atcgcatttc gaacttgaaa
     3481 cgagaaatga agcgaattgg gcggttggat ggtgcgtacg aaccgaaatc gttcaggcga
     3541 ttccggcggt gcggattcga aaataaaaat gcggctaaag gtttgaaata cgatgcggtt
     3601 tcctatgcaa aacgatttcc acacatcgcc aaaatggcga tccggttccg ggatggcaga
     3661 cagtagagat acaaattggc gctgtccgtt ggagtccggc cataagtagc ccaagtcgtc
     3721 tactgcaccc gcaaaaactt catcggacgt attgatagcg atcgaacgta tgaatgcgcg
     3781 gcccggcgtt tccgtgacgc gccaatggat gccgtcaaaa ataagcaacc cattggaatt
     3841 gccgaagtac atgatgccgc gttgatcctg agcgacagcc caattttgta agccggcagg
     3901 gatatcacgc atggtgtagt ttttgatata cggatagcca gtttcttcct gagcgcggag
     3961 cgaagccgtc gtcagtataa gcgcaataaa aagagaggac gtgatgcaac gaggcatgct
     4021 tttttcgttc ataaatcaat cacatgtcgc tgattttttc cttcagaaaa aaacgaattg
     4081 agacaaaact tccgaataag ccgagcagaa taccaaagcc gaccacgatg gccggtattt
     4141 cccacgaaaa ctgcaacgtc ggtaaaagat aacgttggac gatgcggatc aaggcataag
     4201 ctataccggt ggcgatcagg cttcccaaaa caccttgcag gatgccctca agaatgaacg
     4261 gcgcagcgat aaaactgttt ttggcgccaa cgagtttcat cgttttgatc acatcgcgtt
     4321 tggcatagat gctcagtttg atggtgttgg ataccagcag tacggcggcc agtgcggcga
     4381 tacagcccaa tccgatgctg atcatattat acaaatctac tttttcatcc aaagcgagca
     4441 ggagttcttt ttggtagctg acttcttcga cgccgtcaat cgtttctaga gactgggcaa
     4501 cggcgagcac attctgccaa ttgcggtact ccgattgcag acgaattttg aacgaggcgg
     4561 ggagcggatt actttctacg agatcaaaaa tatcatccgc acccatggct tttttgagaa
     4621 ttcgcgccgc atcggatttt gaaataaacg tcaccgattc gacgccttcg atgtcgcgaa
     4681 tggcggcttc tattttttta gattgcggcg tgtccaaagc gcggtcaagg aacgcttcca
     4741 gttccacgcg actacgtatg tcttcgacga gttgattgat gttgagcgtg atgatcagaa
     4801 aaaaactaaa caacgacagc gcaatagcga tgatcgtcac ggcgacaaac gtagataacg
     4861 tcgcgcgccg caaaccgctg aaactttctt tgaaaagata tcctagatgc acgttgattg
     4921 tccatttatt gagaataaca tcggcgcaat atagcgaacc gcatcatgcc gtacaacagg
     4981 agaaatgcga tttgttttcg cccaataaaa aaagcgtacg ctcgagatga gcatacgctt
     5041 ttatagatga aagtatttac ttgattaccg cgggacgttg tcgttttccg taaaagaatc
     5101 cggttcgtca tgcggtttgg tcgtcagtgc gagataaaag aagtatcctg tcgctccgat
     5161 aactacgatg taggtgatca gcataaacgc taatgcggat gaattcatga tgtaaatctc
     5221 ccttcgcgga tacgtttttt atatgcgata aatactaaaa tagatactcc gacaaaaatg
     5281 cccagcaata caaatcgcga ggcattgaca tacatgatgg attcttgtat tgcagcgatt
     5341 tgcacagtat ccgtagtggc agctatttta tcatatagct ttttattgct tattgtatcc
     5401 caaatgtccg gaagactctt cacaaatacc catccgagaa gaatcggtgt gataaatttg
     5461 atgataaatt tgaatacgtt aggtacttta atatcggcgc cgtcggtgat ttctttccag
     5521 cctttatcca taccaaaaac ccatgcaaag aggataattt cgaataaagc aaaaatcacc
     5581 aatgaaacgg tgccggccca ataatcatat tcatcgaaaa ccccgtggtg aaaaaataat
     5641 acggtgggta atcccaatat gagtatggta aagccgaatg cccatgcggc tttttcgcgc
     5701 ttccatccga actcgtccat catgaaaccc atacagggtg tacccatcgc gagggaggat
     5761 gtaattcccg caaaaaataa aagaccaaac caaaacacac cggccaatgc ggcaaggatc
     5821 ggaccccact gcgtaaaaag gtaaggcaat gttttgaaac ccaacccaag cccgccgctg
     5881 gcggtcattt cgatgacttt gtcgatgccg agatatccga cggcgatagg gatcaatatt
     5941 gcactaccga gcaccacctc cacaaattca ttcatccagc ccgcagacat agaattgaga
     6001 gcaatatcat ctttggattt gacgtaggat gcataacaat ggatcgatcc cataccgacg
     6061 gacaatgtaa aaaatatctg cccggcggcg gccagccata ccttgccatc ccaaatcgtg
     6121 tcaaactgag gcgtccataa gaaatttaaa ccgacggtac cgtcatatat agcgccattt
     6181 tccccggcac taagtgtaaa tccgcgaatg gccaaaagaa tgccgaaaag aattaacaac
     6241 ggcatgccga ttttggcaac tttttctacg ccgcccgaca gacctttgga taagatccag
     6301 gtattaagga ataaacacag aagccaaaca ataacgttct caaaaccttc aaaagagaga
     6361 tagtgattga aaaatccggc aacttcatcc tggttttgac cttgaaaact gccgacgagc
     6421 atgtgataaa catacgcgat ggtccatgat tcaagataac aatagtacgc cgctacggca
     6481 aggttggtga aaatcccgaa cacgccgata tatttccaaa atgagcgttt gtccattgaa
     6541 tctaaaatga aaggcgtgct gtggtggccg tttttaccgc cgtaacgtcc catggcccat
     6601 tccacgaaaa gcagcggcaa tcccatcaaa agaaaacaca ccaaatacgg gatgataaag
     6661 gccccgccgc cgttttgtat cgcctgtacc ggaaacctta ggaagtttcc cagtccgacg
     6721 gcattgcctg ccatcgcaag gatcaagcct acacgcgaac cccatgcttc tttgtttgta
     6781 ctcataaacc tctgttcgtt aagtgaaaaa attgaattgt actataacgg aattcattgc
     6841 gtttttgtgt acattagcct atgtacagca taccggcatc gcgcgaatca acgcggggat
     6901 atgtaatccg gcgttcaatt cataagactg ggacaatttt tttaaacaag attggctgca
     6961 ttttccaatc gaaaggtgcg acataataaa aaccgtacgc gcaaaagtca ataacaacaa
     7021 aagctcgacg gataaaatgc atacggaaag gttttgcaaa aggttgcacg atccatcaac
     7081 aaatcccgtt gcgtcgtgtt ttgtattgtt ttttcggtgt atgattctta atcttaacgg
     7141 acatcggttt atcaaatgcc tgattgctac ttcatggtac cgtttacaaa gacttttcat
     7201 cggttgcgcg tcataggttt gacctctttg atcgtggttt ccgtcgtttt gttcggatgc
     7261 aaatctgaca aagatggtga tcgggttgaa gacgccaagg acgaatgcga taatgcacgc
     7321 ggtgtggccg tagatgccaa tggctgtccg tgcgtagatt cggcggcgtt accggaaccg
     7381 cttcctatcg cggcggtacg aatgtacgtg gacaactccg gttccatgcg tgattttacc
     7441 aatcccactt cgctgtttcg ttcaaccata tcggaatgta taagccggat tcagaaaatg
     7501 aaaaccgata acatcgccga cacggtacgt tatcatctca tcgcaagcag tttattgcat
     7561 actgattata atattttgta taaggctatc cgtaccggcg acattatgaa agccaagtcc
     7621 acaccgattt tatccatgat cgaacaggtg caacgccggc atgaagcgca ggacatatcc
     7681 gttttggttt cggattttga attatctgaa aaagatgcgg acaatctcga tcttgcttta
     7741 aaaaccgacg tgcttgccaa aatagccaaa cataatgcag tgggattgta tgcttttcgt
     7801 gaaaagtccg ttgatcgccc gtattacgtg atggtgatcg gcacgtcggc gtccgtactg
     7861 ctctttgatc gggagggcgg gttgcgcgat taccggcata aaatttttct gggtgtgaaa
     7921 tatccggtgc cggaaatagc ttttttgaat tattcccaac gcgatgaaac gacgtggttt
     7981 atcagtcagg atttttgccg aacgatcgaa gacgcttcgc tggccggcaa agggtatatc
     8041 cagtttgcga tgggcttgga cttaaaaaaa tacgaaggtt tgcaactcga ttcggtttat
     8101 ttaactaaaa acacgttgat caaagatgtc gatccgcagc acgttgcgat gcgttggtac
     8161 acggcgcatc agtttaaaaa ccttgctgac gaaaaagaag ccgagcagct cagtacatat
     8221 tcgcacgttg tcgtggtcaa gcttcatcgc ctttttgaaa atacgaataa gtattttttt
     8281 gaattggcca aggcacagga ttttgaacgg ttttatggca gcgatccgag tatgcaggct
     8341 ttggttcacg gttttagaga agcttaccgc ggcgatacgg ggccgtattt ttcagtgaag
     8401 cttaatatcg taaaggaaca ataagtcatg tcggatatgt ttacggcctt atacgaagtg
     8461 tggcacggtc gccaactgga agccgtatgg cggagggaaa tctatccgct tgcggggttt
     8521 ttgatgttgc tgtacggtgt gttttcagcc tggttgtact atttttttct cagtcttcgt
     8581 attcccaaaa cagccgtcga tacgcggttt ttttggtcgt ataatggatt gatggctttt
     8641 ttattcggtc tgatcgcgtt gggtgtattt aaatggaata caacgatcaa cggattaccc
     8701 ggcagtgcga tgctgtttat gctggccaat atggcctatg cgtttatcct cagtctcgta
     8761 gccggtttgg cgatgaaacg ttattctacc aatgcttcca cgattccatt ctgacggagt
     8821 tatcgcatga gtaaactgta tatatttggt atcggcggaa ccggcgcacg ggttattcgt
     8881 tcgttgacga tgctgctcgc gtcgggtgtg cgcatcgaag ccgacgaagt cgtaccgatc
     8941 atcgtggatg tggatctgac caatggaaat acggtacaat cggaagaaac gttgaaatgg
     9001 tatcaaaaga tacgatcacg cgtacaaccc gaatcggagt tttttacgac accgatgcgc
     9061 acgatcggcc agatcacggg cgccgacccg acggcgatgt ttcgttttag ttttgacagt
     9121 acacatcaga aaaaatttcg cgacttcata gattacagca aactctcggc ggaagaccag
     9181 tttatggtca atcttttgta ttcggaagag tatttggatg acgaactgac ggaaggcttc
     9241 aaaggcaagc ccaatatggg ttcggtggcg ctcaatacgc tgacctcaat ggatgaatac
     9301 gatcaatttg tcaattcctt tcagccggga gatcgtattt ttgtcgtcag ttcgattttc
     9361 ggcggtaccg gtgcggcggg ttttccgatt ttgctcaaaa atttttgttc gcaaaaatcc
     9421 gatcccaaac tgcagaaagc gctgaaaggt gcgctgacgg tcatgccgta ctttggcgta
     9481 caagcggaca acgaaagtca aattgattcg aatacgtttc atgctaaaac caaagccgcg
     9541 cttgcgtatt acgaaaaaaa tctctccggt ttgcatcatt tgtattattt agccgatacg
     9601 atcaaagagt tgtatcccaa ttttgaagga tccaccgggc agcgtaacaa agcccacgtg
     9661 attgaggttt taggcgcact ggctattatt gattttgcgc ggcagacgtt tgaaacatcc
     9721 ggcgcaaaaa ccttatgcca tgaatttgcc ctcaaagaag acggcgaagt actgaccttt
     9781 accaatcttc ccgatccggc gcaagcgttg atcaaagcgc cgttgatccg gtttttgtat
     9841 ctttattata tcatgcaatt tttgctgcgt tccgagcgaa aagatgcctg gataaaaaat
     9901 atttttggta cggcatcggc gctcaatgac gaagatgcca aattgctgca aaagtttttg
     9961 tacatgtatg ccgactggat aacagagttg gccgaaaaca aacgcgcttt tgagccgttt
    10021 tatatgcagc agtttgccga gtcatttgct cacgccttgg taaaaggtta tcaactggag
    10081 ccgggcggac ttttccgaac aggactggac gataatttta tcatctcggc gctgggatcc
    10141 aatagccgtc agttgccggc cacgctgacg acgatgggca aattgatcaa agccggttat
    10201 gccgcgacgg acgcggtgat acaacgtttt tataaataaa ttcaataacg ttttggagcc
    10261 cgcatgggat atcttctcaa tctgaaaccg gcacgcagtg tggcgcagga tcactggacg
    10321 gatacgaccg tgtttcatcc caatatgatt aattctatcg aagacaatca gagcgacaaa
    10381 gtcgagcgtc ccattgcgtc tattccttct ccgtttgcgc agatgcattt attcgattac
    10441 gcatttcgct ccgtcaacgg gcgtggcgaa atgagcggga attcactcta tcatcggctc
    10501 atctcctata cgtttgatgt tatggagctg ctctttcatg cgaataatat cggcggggtg
    10561 ggcggtaaac tggaagtca
//
LOCUS       DAOQNQ010000094        10540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2265_length_10540_cov_6.015069, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000094 DAOQNQ010000000
VERSION     DAOQNQ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10540
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2265_length_10540_cov_6.015069"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PK754_07480"
     CDS             complement(<1..669)
                     /locus_tag="PK754_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09382.1"
                     /translation="MKRILSAIAGFCYRHSTSVLIAVGLITVVSTYYMMQIKISTNNF
                     DLLPQNSKLIKEFWEVNEDFGAQETHILLIETNDSLPAEPEIIRDYAERLDAAFMASG
                     LVKQVNYRITDDQKSFVEDFFIRNALLYLSPQDLDSVVRRLDDANIEKAILNAKNILN
                     APIPPDPLLKRVLREDPLLLSELFVPYIEKMLGPQNASLIREKESYYMSKDKRTLLMF
                     VKPDG"
     gene            837..3050
                     /locus_tag="PK754_07485"
     CDS             837..3050
                     /locus_tag="PK754_07485"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNB09383.1"
                     /translation="MRWFIGISLLAMVLPAMAQHGHSLKISETDDLSAADASFYLQRA
                     SELLEQREYASALRHFVFAGEIEPSLYRQFVFQYKLAKAYRGLRQMEKAIQYFSHTAA
                     DSAFGDFALVSLGELYLDVDSTNQARSVARRLLRRFPKSALIPHGYYLLLKIADIQKD
                     DAVWKATMTDLRRILSDYPKLQMQWEPDLLQREARFFIRNRENEQAQTILRRLQNDYP
                     YSQEAYEAWPQLFSFYPSKNNPPPVDDLINALQVRMMQGHHTEALNMIAALRTWYIDD
                     FAHKEIDFMTARIYFAQGRYDLAIPRFANLWSDHASAEALFQMGRAARYADEIERSIH
                     AYGQYLQHGKLNDAWSSYIRFEMANNRAVRFDSAAIGEVNDGYRSVYQNASQRSHYAV
                     ASRFREGLNWYIAGTYDSAIVYWEKYMRSTPSQKTRAQYWIARAYAKKNDIQKAKALY
                     ESITHTKGDEYYTTLAHMRLTSHDLRAFYFTDTTDDGNKTKKIFNPVSTPDIGNDPRL
                     DRVAMAIRIGEVAWGEQEAEPLVNDYITRRDRGEKFRDYLESLHAYDLAVDVNVALKK
                     KYRSYYKIYNDDVRMFYPLYYRPLVKHHATAYRLDEALIFALIKSESAFRTYALSPAR
                     AIGLMQIMPFTGKALAAETGMPDFQPSSLHEPDVSIHLGSHYLRQLADMYKNYIPAVL
                     GAYNAGPHRATFWMKAFNDEEPDIMPEVVEIFETETYIKRILVDRYKYDLKLHAK"
     gene            complement(3043..4461)
                     /locus_tag="PK754_07490"
     CDS             complement(3043..4461)
                     /locus_tag="PK754_07490"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNB09384.1"
                     /translation="MFYVESIYSDIRLRENAERISEHIIQGLWNYLFLRMDDLTTVDG
                     HKIRILDKGRWHSGSGPDFRQATLRIGDTILCGDVEIHWQTTDWLKHGHERDPAYFRV
                     ILHVVYRHDKDLHQGAPYTLEIKDRLSDTLKTLTEKVMLLDADQDTLFCSDFVPSIEP
                     EFVTQWVQENGAHRLRRKTEQLKNLQKEKQWTDEQLLYYVLCDAMGFTKNRLPFCMLA
                     ERVPYDVVYASANGVDSSVALMRVQALLIGAAGLLPDEATMSADPKLWPYAGRLWDLW
                     SALSSQHQITPLHAEVWIRDRLRPKNYPLPRMAALAGLLVTHLKDGLSVAVIRLFSQC
                     DTPPVILKNLHRILKVRAYGYWGEHVWWNVQTGRPYSDLIGKQRAYEIIINAIVPYVT
                     LYAQRLGDKKLYETVMTCMKTWVAHEHNHIIGWMEKRLPGMPDRINAEYAQGLIELHK
                     KCRVRNCSDCRIFQRMVYSDFI"
     gene            4528..4613
                     /locus_tag="PK754_07495"
     tRNA            4528..4613
                     /locus_tag="PK754_07495"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4562..4564,aa:Leu,seq:caa)
     gene            complement(4842..5978)
                     /locus_tag="PK754_07500"
     CDS             complement(4842..5978)
                     /locus_tag="PK754_07500"
                     /inference="COORDINATES: protein motif:HMM:NF016993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaI"
                     /protein_id="HNB09385.1"
                     /translation="MIKAQVSTFDDWIDLFQEWKKDIGYDEKTFKRFLPNYKLEPKYE
                     ALKYNEIEFGDFRGDRKWERVLDIPNQGIRDALQQLIVFQGDTEFASVEQQRYLVDRA
                     PSDYDLYALMRINAEEMRHGWQMSHILVTHFGDAGRKEAMKLLERRAFEQKRLLGSFN
                     EVMENWLDLYTYTEFIDRDGKFQLKMLSHSAFMPLSMSMNPMLKEESFHLGTGNNGLK
                     RIIKAGKIPVEIIQKYFNKWVPTAYDLFGTDNSSNAHWAYVWGLKGRFNEHETDEEVQ
                     KDKLNQLARDLYIAEISKLVEDMNTLIPREMPKLIVPDVKFNRSIGLHAKQPYTVDGR
                     LLSEEQYKTYRETVLPTDKDRELLKDIFKENDWIEFKPFKGAEA"
     gene            complement(6014..6712)
                     /locus_tag="PK754_07505"
     CDS             complement(6014..6712)
                     /locus_tag="PK754_07505"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNB09386.1"
                     /translation="MNTAVTKSSVRSTVPKRRLYMPENAGDQARFHLRLNDILERSAT
                     LFSEQGYHNTSMRDIAGAMHTSLAGLYYYFKTKEELLFLISQYSFNSVIGSLHEKLAH
                     VHDPVLKLHILVQNHLLYFVTHLDAMKILALESDSLTGEYHRIINEKKKNYLKILETI
                     LSEMVISKNNKSARRTTPEIKIAALALFGMMNWTYTWYNPKKRTHNTTNIARISKQMV
                     DIFLSGFLQKNLQS"
     gene            6908..7711
                     /gene="rsmA"
                     /locus_tag="PK754_07510"
     CDS             6908..7711
                     /gene="rsmA"
                     /locus_tag="PK754_07510"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNB09387.1"
                     /translation="MSSVAVKTPAPKRKFSQNFLTNPHYVRKIVDAANLSSGDTAVEI
                     GCGKGALTELLLESGATLHGVEIDPDMIRILTERFSQHPRFTLHHQDILTFDFHGMAS
                     GFPLTVIGNLPYHISSPILFHLMESGPIIRQALIMVQKEVAERIVGKPHSKDYGILSI
                     FCQYHAACEKLFDLPPGAFFPAPKVTSSIVRMTFHRDMTPWPHYPLFVKIVKTGFNQR
                     RKMLRNSLSSFEGINNVTIDLSQRPENLSVEDFIRLTDELAMHQRHYAA"
     gene            7701..9062
                     /gene="mgtE"
                     /locus_tag="PK754_07515"
     CDS             7701..9062
                     /gene="mgtE"
                     /locus_tag="PK754_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNB09388.1"
                     /translation="MRPKAEIEIDKELIDDIKDLVDSKAELLVRNILSDLHPSDISEI
                     LDHLDEEERNYVFQVIEPEKASEVILELNATSRENVLDNLDEEQITEIIEEMQTDDAA
                     NIVQELDDETAKRVLDQLDYEDQQEVKELLKYDEDTAGGLMQLEFVSVIDTSTMVQAT
                     NEARKKAADVEKIHDIYVVNSYGHLVGSISIEKLLVEPAEKHVAEVMHQEIPFAKTDE
                     DQEEVAHLFKKYDLIALPVVTRAGKLVGRITIDDIVDVIEQEASEDVSKMAGSVDEDV
                     SEQSTFRISKSRLPWLFTALAGETVAAFVLSRFESSLNAILALAFFVPIIMAMAGNIA
                     IQCSSIVIRGLATGEISIIDIQRQIFKELRVAAINGFVLGIALAMIVALWIGNMQVAF
                     VVGIALICVIFFAAFNGTLFPLLLKKLNIDPALAAGPFVTMTNDTIGILIYLGIATLL
                     LIR"
     gene            complement(9076..9447)
                     /gene="acpS"
                     /locus_tag="PK754_07520"
     CDS             complement(9076..9447)
                     /gene="acpS"
                     /locus_tag="PK754_07520"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNB09389.1"
                     /translation="MIAGIGTDIVEISRIRQSCEAYGDRFIHRVFTPGEITYAYSKKD
                     PYPSLAVRFAVKEALIKAARVGKFHPHGWLDAEVITDARGVPSIELYGELKKELAGQQ
                     IHITVSHADHYATATVIIEKK"
     gene            complement(9444..10076)
                     /locus_tag="PK754_07525"
     CDS             complement(9444..10076)
                     /locus_tag="PK754_07525"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNB09390.1"
                     /translation="MSATPTALTPELTLYCRLSACVAIHDAEPMRESIATALDAGYDV
                     LRIKEVILQNYLFSGFPSAIEGLIVLGATLKHRGIADNNWVEMRTEDQIKSDGMALCA
                     MIYDKNFDKLMHNMQSLSADIRDWMIFEGYGKVLSRPILTAKERELCVISALAAMGRE
                     RQLISHLRGAVHVGASDGEISEALQSISQTAARGKISEAMRLWDETRSRS"
     gene            complement(10086..>10540)
                     /locus_tag="PK754_07530"
     CDS             complement(10086..>10540)
                     /locus_tag="PK754_07530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB09391.1"
                     /translation="IAVDIEKQIERALHLLRIKYKNKITFHKDYTPLPSIKGLPGPLT
                     QVMVNLLQNSAQSIQKEGHIWIRTWRGPDGIRVSIKDDGQGIAEEHISQVFDPGFTTK
                     PEAEGTGFGLAISQAIIQKHGGSIAVTSVLGKGTEFIISLPWEQALSA"
BASE COUNT         2774 a         2528 c         2362 g         2876 t
ORIGIN      
        1 accatccggt ttgacaaaca taagaagcgt acgtttgtct ttggacatgt agtagctctc
       61 tttttcccga atcaatgagg cattttgagg gcccaacatt ttttcgatat acggcacgaa
      121 cagttcggac aaaagcagcg gatcttcgcg taaaacgcgc tttagcagag gatccggcgg
      181 aatcggcgcg ttgagtatat ttttagcatt aagaatggct ttttcgatat tggcatcatc
      241 taagcgtcga acgaccgaat ccaaatcctg cggcgaaaga taaagcagcg cattgcgaat
      301 aaaaaaatct tcgacaaacg atttttgatc gtccgtgatc cggtaattga cttgtttgac
      361 caatcccgaa gccataaagg ccgcatccaa tcgctcggcg taatctctga tgatttccgg
      421 ctcggcaggc aaagaatcgt tggtttcgat aagcaggata tgtgtttcct gcgcgccaaa
      481 atcttcgtta acttcccaaa attctttgat gagtttggaa ttttgcggca acagatcaaa
      541 attattagtg cttattttga tctgcatcat gtaatatgta gagacaacgg ttataagccc
      601 gacggctata agaacggacg tagagtgacg ataacaaaat cctgcaatgg ccgacaaaat
      661 acgcttcata cgatactcct aaatagtggc gcaaaataga atatttaggt tatttgtgca
      721 atttaattca cctctttatt gatcaccaaa acatggactg tttaccgctt tcacattgaa
      781 ataaagctcg cgaacatcta cttttttata tgagcataca atatacatta cagcccatgc
      841 gatggtttat aggaatcagc cttttggcca tggtattacc cgctatggcg caacatggcc
      901 attccttaaa aatttccgag acggacgatc tctccgcggc cgatgcgtct ttttacctac
      961 aaagagcctc cgaactttta gagcagcgcg aatacgcgtc ggcattacgc cattttgttt
     1021 ttgccggcga aatcgagcct tcattatacc ggcaatttgt ttttcaatac aaattggcca
     1081 aagcgtatcg cggtcttcgc cagatggaaa aagccattca atatttctca cataccgcgg
     1141 ccgactcggc gtttggcgac tttgcgctcg tatctctcgg cgagttatat ttggatgtgg
     1201 atagtacgaa tcaagcccgg tcggttgcac gccgactgtt gcgccgtttc cccaaatccg
     1261 cacttattcc gcatgggtat tatctcctgc tgaagattgc tgatattcaa aaagatgacg
     1321 cggtatggaa agcaacgatg acagatctac ggcgcattct gtcggattat ccgaaattac
     1381 aaatgcagtg ggaacccgat ctcctgcaaa gagaagcgcg tttttttata cgcaatcgtg
     1441 aaaacgaaca ggcgcagacg attttgcgac gtttacaaaa cgactatcct tactcgcaag
     1501 aggcctacga ggcttggccg caattattta gtttttatcc gtccaaaaac aatcctccgc
     1561 ctgtagatga cttgatcaat gcactgcagg tgcgcatgat gcaaggtcat cataccgaag
     1621 cgctcaatat gatcgccgct ttacggacat ggtatataga tgatttcgca cataaagaaa
     1681 tagattttat gacggcgcgc atttatttcg ctcaaggacg gtacgatctg gcgataccgc
     1741 gttttgccaa tctatggtcg gatcatgcgt ctgcggaagc gctttttcag atgggtcgcg
     1801 cagcacgtta tgcggatgaa atcgaacgat ccattcacgc gtacggtcaa tacctgcagc
     1861 acggaaaact caacgatgca tggtcgtcat atattcgttt tgaaatggcc aataaccgtg
     1921 cggtacgctt tgattccgcc gcgattggtg aagtcaacga cggataccgt tccgtatatc
     1981 aaaacgcctc ccaacgttcg cactatgccg tcgcatcgcg ttttcgcgaa gggctcaatt
     2041 ggtatatcgc cgggacgtat gacagcgcga tcgtttattg ggaaaaatat atgcgcagta
     2101 caccgtctca aaaaacacgc gcacaatact ggatcgcccg cgcgtacgcc aaaaaaaatg
     2161 acatacaaaa agcgaaagcg ttgtacgaaa gtattacgca caccaaaggg gatgaatact
     2221 ataccacatt agcacacatg cgtttgactt cgcatgacct gcgcgctttt tattttaccg
     2281 atacgaccga tgatggtaac aaaaccaaaa aaatattcaa ccccgtaagt acccccgata
     2341 tcggtaatga tccgagattg gaccgcgtag cgatggctat tagaatcggt gaagtggcgt
     2401 ggggtgaaca ggaagccgaa ccattggtaa atgattacat cacccgccgc gatcggggcg
     2461 aaaaattccg tgattatttg gaaagcctgc atgcgtacga tctggccgtg gatgtcaatg
     2521 tcgctctgaa aaagaaatac cgtagctatt ataaaatata caatgatgac gtacgcatgt
     2581 tttatccgct gtactatcgt ccgttggtta agcatcatgc gacagcatat cgccttgacg
     2641 aagcgctgat ctttgcattg atcaaatcgg aaagcgcttt tcgcacgtac gcattgtccc
     2701 ccgctcgcgc gatcggtttg atgcagatca tgccgtttac gggcaaagcg ctggcggcag
     2761 aaaccggtat gcccgacttt caaccttcgt ctttgcatga accggacgtc agcattcacc
     2821 tcggatccca ctatcttcgg caacttgctg atatgtataa aaattatata ccggctgtat
     2881 tgggcgcata caatgctggc ccacatcgcg ccacgttttg gatgaaagcg tttaacgacg
     2941 aagaaccgga catcatgccg gaggtggtgg aaatttttga aacggaaacg tatatcaaac
     3001 gcattctcgt ggatcgctat aaatacgatc tgaaactgca cgctaaataa aatccgaata
     3061 caccatgcgt tggaatatgc ggcaatcact gcaattgcgc acacggcatt ttttatgcag
     3121 ttcgatcagc ccttgcgcgt attcggcatt gatccgatcc ggcatgcccg gtaaacgttt
     3181 ctccatccag ccgatgatat gattatgttc gtgcgccacc cacgttttca tacatgtcat
     3241 gacggtttca tacagttttt tatcccctag gcgctgcgcg tacagcgtga catacggaac
     3301 gatcgcattg atgatgatct cgtatgcgcg ttgctttccg atcagatcgg aatacggacg
     3361 gcctgtttgg acattccacc aaacgtgttc cccccaataa ccgtaagcac ggacttttag
     3421 aatacgatgt aagtttttaa gaattaccgg cggcgtgtcg cattgagaaa atagtcgtat
     3481 cacggcaacg ctcaaaccgt ctttgagatg agttacaagc aatcccgcaa gagcggccat
     3541 gcgcggaaga ggataatttt tcgggcgaag ccggtcgcgt atccaaacct cggcatgcaa
     3601 cggagtaatt tggtgctgcg aagataaggc cgaccacaaa tcccataatc gccctgcata
     3661 aggccacaac ttgggatcgg ctgacattgt cgcctcatcc ggaagcaaac cggccgcacc
     3721 gatcagtagc gcttgcacac gcatcagtgc gaccgatgaa tctacaccat ttgccgaagc
     3781 atacaccaca tcataaggca cacgttcggc taacatacaa aaaggaagcc tgttttttgt
     3841 aaagcccatc gcatcacaca gaacatagta gagaagctgc tcatccgtcc actgtttttc
     3901 tttttgtaag tttttaagtt gctcggtttt ccggcgcagc cgatgcgcac cattttcctg
     3961 cacccattgc gttacaaatt caggttcgat ggacggcaca aagtcactgc aaaaaagagt
     4021 atcctgatcc gcatccaata gcattacctt ttccgtcagc gttttcaaag tatcgctcag
     4081 tcgatctttg atttccagcg tgtatggtgc cccttggtgc agatctttat cgtgacggta
     4141 cacaacatgt aagatcactc gaaaataagc cggatcgcgc tcatggccgt gtttgagcca
     4201 gtccgtagtt tgccagtgaa tttctacatc gccgcacaat atcgtatcgc cgatgcgcaa
     4261 agtcgcctga cgaaaatccg gcccgctgcc gctgtgccat cgccctttgt ctaaaatgcg
     4321 aattttgtgc ccatccacgg tcgtcaggtc atccatacgc aaaaataagt aattccataa
     4381 accctgtatg atgtgttcgg aaatgcgttc ggcattttca cgcaaccgta tgtcgctata
     4441 aatggactcg acgtaaaaca taaaaaatct gttgcaaaaa atggaaaact ggctatattc
     4501 atgccacgtt ttttgaaacg atagaacgcc gaagtggtgg aattggtaga cgcgctggac
     4561 tcaaaatcca gttgggctca aacccagtga gagttcgagt ctctccttcg gcaccccaaa
     4621 gccatgcata acactgtgac gcatggcttt tttatttctc tcgattaaaa acctcgcatc
     4681 gtatttatta taagatacac cacgatagtt tggattttat gtcaatctct gacaaaaaaa
     4741 gccttaaagt tcatttaaga cgcagtatcg aaacgacagc ccaataaaaa aaaccgcgcg
     4801 gtctcccgag cggttttttg acgaaaaaaa tatgaatcgg atcaggcttc cgcacctttg
     4861 aaaggtttga attcgatcca atcattttct ttgaaaatat ctttcaatag ctcacggtct
     4921 ttatcagtgg gcaacaccgt ttcgcgatag gtcttgtatt gctcttcact caaaagacga
     4981 ccgtccactg tgtacggctg tttggcatgc agaccgatgc tgcggttaaa ttttacatcg
     5041 ggcacgatca atttgggcat ttcgcgaggg atcagagtat tcatgtcctc cacgagtttg
     5101 ctgatctcgg cgatatacag atcgcgtgca agctgattga gtttgtcttt ctgcacttct
     5161 tcatccgtct catgttcatt aaaacgacct ttgagacccc agacataagc ccagtgcgcg
     5221 ttggacgaat tatccgttcc gaaaagatcg taggcggtag gaacccattt gttaaaatat
     5281 ttttgaataa tctcgaccgg tattttaccc gctttgatga tacgcttgag gccgttatta
     5341 cctgtgccaa ggtgaaatga ttcttctttg agcataggat tcatactcat cgagagcggc
     5401 ataaatgccg aatggctcag cattttgagc tgaaacttac cgtcacgatc tatgaattcg
     5461 gtataggtgt aaagatcgag ccagttttcc atcacttcat taaatgaacc caataaccgt
     5521 ttttgttcaa acgcacggcg ctccaggagt ttcatcgctt ctttgcggcc ggcatcgccg
     5581 aagtgcgtca ctaagatgtg actcatttgc caaccgtggc gcatttcttc cgcgttaata
     5641 cgcatcagcg catagagatc gtaatcgctg ggcgcacgat ccaccagata ccgctgctgt
     5701 tcgaccgagg caaattccgt atcgccttgg aatacgatca actgctgcaa cgcatcgcga
     5761 ataccctgat tgggtatgtc cagcacgcgt tcccacttac gatcacctct aaaatcgccg
     5821 aattcgattt cgttgtactt cagcgcttcg tacttgggtt ccaatttgta gttggggaga
     5881 aaccgcttaa atgtcttttc atcatagccg atgtcttttt tccattcctg gaaaagatca
     5941 atccaatcgt caaatgtgga aacctgtgct ttgatcataa cgcgaccttt cggttaagag
     6001 ttatttatgt gaattatgat tgtaaattct tttgtaagaa accgctcaga aaaatatcca
     6061 ccatctgctt ggaaatacgg gcgatattgg tggtattgtg ggtacgtttt ttggggttgt
     6121 accacgtata agtccagttc atcataccga aaagagccaa cgcggcaatt ttgatctccg
     6181 gcgtcgtccg tcgggcagat ttgttgtttt tggagattac catttcggag agtatggttt
     6241 ccaaaatttt aagataattt ttcttctttt cattaataat gcgatgatac tctccggtca
     6301 gcgaatcgct ctccaatgcg agtattttca tagcgtcaag gtgcgtgaca aaatagagca
     6361 aatgattttg aacgaggatg tgcagcttca aaaccggatc gtggacgtgt gcaagttttt
     6421 cgtgcaacga accgataacc gagttaaatg agtactgcga aatcaaaaac aaaagctctt
     6481 ctttagtttt aaaataataa tacaaaccgg ccaaactcgt atgcatcgct ccggctatgt
     6541 cgcgcatgga cgtattatga tatccttgct cggaaaaaag agtcgctgag cgttccagga
     6601 tgtcgttgag ccgcagatga aatcgcgcct ggtcacctgc attttcaggc atatataacc
     6661 ggcgtttggg cacggtactg cgtacggatg atttggtgac ggccgtgttc ataaatcagg
     6721 tttgattcgg ttatacaatt cgaacgaacg ttcgaaatct aagcgaccgt cggcatattg
     6781 tcaaatttat ttttttacgg caatcccgtc gtttatttta tccgttcatg actttcccgt
     6841 tgaaacttcc tgtcatgatt cgtatatatt caaaatacat ctccattttc caaatttcat
     6901 ttttttcatg tcttccgttg ccgtcaaaac gcctgcaccc aagagaaaat tcagtcagaa
     6961 ttttctgacc aatccgcatt atgtgcgaaa gatcgtggat gcggcaaatt tatcctccgg
     7021 cgacaccgcg gtcgaaatcg gatgcggtaa aggtgcgctg accgaacttt tgctggagag
     7081 cggcgcgact cttcacggtg ttgaaatcga tcccgatatg atccgtattc tcacggaacg
     7141 tttttcccaa catccgcgtt ttacgttgca tcatcaggac atccttactt ttgattttca
     7201 cggtatggct tccggttttc ctttgaccgt tatcggcaat ctcccgtatc atatttccag
     7261 tccgatttta tttcatctga tggaatccgg gccgatcata cggcaagctc tgatcatggt
     7321 acaaaaagaa gtcgccgaac gcatcgtggg caaaccgcac agcaaagatt acggcattct
     7381 ttccattttt tgccaatacc atgcagcttg tgaaaaactt tttgatttgc cgcccggcgc
     7441 tttttttccg gcacccaaag tgacgtcatc cattgtacgg atgaccttcc atcgcgatat
     7501 gacgccctgg cctcattacc cgctttttgt aaaaattgtc aagaccggat ttaatcagcg
     7561 tcggaaaatg ttgcgtaatt ccctttcttc gtttgaaggc atcaacaacg tcacgataga
     7621 tttgagtcaa cgcccggaaa atctttcggt agaagatttt atccggctaa ccgatgaact
     7681 tgctatgcac cagaggcatt atgcggccta aagcggaaat cgaaattgac aaagaattga
     7741 ttgacgacat caaagatctc gtcgattcca aagcagaact tcttgttcgt aatattctga
     7801 gcgatctgca cccgtccgac atctcagaaa tcttggatca cctggatgaa gaggaacgga
     7861 attatgtttt tcaggtgatc gaacctgaaa aagcctcgga agtcattctt gaactgaatg
     7921 ctacgtcgcg cgaaaacgta ctcgataatc tggatgaaga acaaatcacc gagatcatcg
     7981 aagagatgca aacggacgat gcggccaata tcgtacagga actcgacgac gaaacggcca
     8041 aacgcgttct agaccaactt gattatgaag atcagcagga agtaaaggaa ctgctgaaat
     8101 atgacgagga tacggccggc ggtctcatgc agcttgagtt tgtatccgtg attgacacca
     8161 gtacgatggt gcaagcgact aacgaagcgc gtaaaaaagc agcggacgtc gaaaaaatac
     8221 acgatatcta tgtcgtcaat agctacggtc acttagtcgg ttcgatctcc atcgaaaaac
     8281 tactcgtgga accggcagaa aaacatgtcg ccgaagtaat gcatcaggaa attccgtttg
     8341 ccaaaacaga cgaggatcag gaagaggtcg ctcatttatt caaaaaatac gatctgatcg
     8401 ctttgccggt cgtgactcgt gccggtaaac tggtgggtcg tatcaccatt gacgatatcg
     8461 tggatgttat cgagcaggaa gcttccgaag acgtctccaa aatggctggc tccgtggatg
     8521 aagatgtcag cgagcaatcc acatttcgta tttctaagtc gcgtttgcct tggcttttta
     8581 cggcactggc cggtgaaacg gttgccgctt ttgttttgag ccgattcgaa tcgtcgctca
     8641 acgcaatatt ggcattggca tttttcgttc cgatcattat ggcaatggcc ggcaatatcg
     8701 ccatacaatg ctcatccatc gtcatacgtg gtctcgccac cggtgaaatc agcatcattg
     8761 atattcagcg ccaaatattc aaagagctgc gcgttgcagc catcaacggg tttgtattgg
     8821 gtattgcttt agcaatgatc gttgccttgt ggatcggaaa catgcaagtg gcctttgtgg
     8881 tgggtattgc actgatctgc gttatatttt ttgccgcctt caacggcacc ttattcccac
     8941 tattgctcaa aaaactcaat attgatccgg ctttagctgc aggacctttt gtcactatga
     9001 ccaacgatac gatcggtatt ctgatttatc tgggtatcgc gacactgctg ctgatacgat
     9061 agaaattttg aagcattact ttttttcgat gatcacggtc gccgtggcat aatgatcggc
     9121 atgcgaaact gtaatgtgta tctgctgtcc cgccaattct ttttttaatt caccataaag
     9181 ttctatcgaa ggcacaccgc gtgcatccgt gatgacttct gcatccagcc atccgtgggg
     9241 gtgaaattta cccacccgcg ccgctttgat caacgcttct ttgacggcaa atcgaacggc
     9301 caacgacgga tacggatctt ttttagaata cgcatatgtt atttctcccg gggtaaatac
     9361 ccggtgaata aaacggtccc catacgcttc acaactctgg cgtatacgcg atatttcgac
     9421 aatgtccgtg cctatccctg cgatcatgac cgactccgtg tttcatccca taggcgcatc
     9481 gcctcagaaa tttttccgcg tgcggcggtt tgtgaaatcg attgaagcgc ttccgaaatc
     9541 tcgccatcgc tcgctccgac gtgtacggca cctcgcagat gggaaatgag ttgcctttct
     9601 cgacccatcg ccgccagcgc actgatcacg cacagctcgc gttctttggc cgtcagtatc
     9661 ggacgactaa ggactttgcc gtatccttca aaaatcatcc agtctcggat atcggcgctg
     9721 agcgattgca tattatgcat aagtttatca aaatttttat cgtaaatcat cgcacacaag
     9781 gccataccgt ccgatttgat ttgatcttcc gttcgcatct ctacccaatt gttatcggcg
     9841 ataccgcggt gtttgagcgt cgcgccaagt acgatcaaac cttcgatcgc tgatggaaat
     9901 ccgctgaaaa gataattttg cagtatcact tctttgatgc gcaatacgtc atacccggca
     9961 tccagtgccg tagcaatgct ttcgcgcatc ggctcggcat cgtgtatggc gacgcacgcc
    10021 gataggcggc aatacagagt taattcggga gttaaagcgg taggcgttgc actcataacc
    10081 gttatttatg cgcttagcgc ctgttcccac ggcaggctga tgataaattc cgtaccctta
    10141 cctaaaacac tggttacagc gatcgaaccg ccatgctttt gaataatagc ctgcgaaata
    10201 gcgagtccaa atcccgtgcc ttccgcttcc ggttttgtcg taaaacccgg atcgaaaacc
    10261 tgagaaatat gttcttcggc tatcccttgc ccgtcatctt tgatggatac gcgaatgccg
    10321 tccggtccgc gccaggtgcg aatccaaata tggccttctt tctgaataga ctgcgccgag
    10381 ttttgtaaaa gattgaccat aacttgcgtt agcggtcccg gtaaaccttt gatggacggt
    10441 aacggagtat aatctttatg aaaagttatt ttgtttttgt atttgatgcg aagaagatgc
    10501 agagcgcgtt cgatttgttt ttcaatatcc accgcgatcc
//
LOCUS       DAOQNQ010000095        10341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2330_length_10341_cov_6.274451, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000095 DAOQNQ010000000
VERSION     DAOQNQ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10341
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2330_length_10341_cov_6.274451"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PK754_07535"
     CDS             <1..333
                     /locus_tag="PK754_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09392.1"
                     /translation="DFIAVGLTGAEYLLKKKVKLVGVDYLSVESFHAEFDHPVHKKLL
                     GSHVVVVEGLNLSDVRPGEYELICMPLKIKHGDGTPVRAALRDLPNAKVKSKPKKQTA
                     ANKKRKKK"
     gene            complement(457..939)
                     /locus_tag="PK754_07540"
     CDS             complement(457..939)
                     /locus_tag="PK754_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09393.1"
                     /translation="MLSVLLALFYLSCSGSKNIRKTTETKSDAEAPPVMVESPKVMRW
                     YIVVEQKAGLYKRYVELSYDGQMSQLTEGGRGPASVVFFAADTLRRLDRDIAEMIGEK
                     VSTSDRQDAILCLTVYSESGDAVLDTCWTQTSWENSSASARRLVEWISRQTDNFISIK
                     "
     gene            complement(974..2260)
                     /gene="glyA"
                     /locus_tag="PK754_07545"
     CDS             complement(974..2260)
                     /gene="glyA"
                     /locus_tag="PK754_07545"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNB09394.1"
                     /translation="MEHIHQFDPELYKAIADEKVRQNTTLEMIASENFVSKAVMEAAG
                     TVMTNKYAEGYPGNRYYGGCEFVDVAENLARDRAKELFGAAYANVQPHSGSQANMAVY
                     FTLIKPGDTVMGMDLAHGGHLTHGSPVNFSGKMYKVVSYGVKKETGRIDYDQMVRVAR
                     EHKPKLIIAGASAYTRFFEYEKFRAIADEIGAYLMADMAHPAGLIAAGLHPSPVPYCD
                     IVTTTTHKTLRGPRSGLILMGKDFENRLGITAPKSGRLRMMSEILDSNVMPGIQGGPL
                     MHIIAAKAIAFKEALQPSFKEYARQVMANAKALAEQLVNLNYTLVSGGTDNHLVLVDL
                     SNKNITGKKAEQTLEHAGITVNKNMVPFDVQSPLVTSGIRIGTPALTTRGMKESEMRI
                     IAGFIDRAIRHPDQMAELHKIKNEITDLTKNFPLYA"
     gene            complement(2337..3842)
                     /locus_tag="PK754_07550"
     CDS             complement(2337..3842)
                     /locus_tag="PK754_07550"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09395.1"
                     /translation="MKNSIFVAGMIFFLISCGQEKKHMSIKEFPVAVDSIFQKSCAIA
                     GCHLPDEHDLAKALHLPNNLDLSSWSGLFEGGENGAAVVAYRADKSHMMGHIRGVMDP
                     RMPPNYAPYNKDTLVASDVTIIQSWIEDGAPSLEGNIPFEHVRNKIFTTNQINDIVSV
                     LDARTHNIMRVFDVGDRPQNESPHGIEVSRDGKYFYTSMIATGDVFKYDAKTGELIDK
                     AALLDPVALIKLSQDGSKLYVTTKFEVNNTGNNGRITVLNTETMSEIKSIPVGVSPHG
                     INLSKDGTLLYVSAVYSDRIYVINTQADTLATHFDVADDVTPTPKYEPYHIGMGPKNN
                     SGYENLLFITCRKNAQVRIFQRTKTNNGNTFTFKQAINVGHNTNAKPIQLDVLPDGSA
                     VFVANSNDSSITVIKKNAEGYYKETDITAQTSDDGKTFHRLAQPNGVSVSPDGSLVYV
                     SNRNKDGAIIPHHGGSGGTGLITVIDVSTNKILKTLEVDPDAYSVFVSYPR"
     gene            complement(3927..5120)
                     /locus_tag="PK754_07555"
     CDS             complement(3927..5120)
                     /locus_tag="PK754_07555"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNB09396.1"
                     /translation="MKLNKEIVIVAGARTAFGKFGGTLKDFSAIQLGVFAAQETIKRA
                     GVKPEDVDHVVFGNASQTSADAIYMARHIALKAGLPQTTPALTLNRLCGSGFEAIIEG
                     ARQILLGESTMVLAGGTESMSQAPFVVRGTRFGVNLGQPMKFEDSLWEALSDPHCGFS
                     MAQTAENLADKLKISRTDCDLYAYLSQMRAKDAILGGRLKDEIAPIEIKTRKGVKVFD
                     TDEHPRPETTVEMLQTLAPYFKKDGTISAGNASGICDGAAAVLITTAEIAKAKGLKPL
                     GRLISWGHAGVDPSIMGIGPVPSSQKALADAGLKVSDMDIVEINEAFAPQYIACEKEL
                     GLNREITNVNGGAIALGHPLAASGARLTLTALYELKRRGKKYGLASACIGGGQGVAVV
                     IEALS"
     gene            complement(5261..6565)
                     /locus_tag="PK754_07560"
     CDS             complement(5261..6565)
                     /locus_tag="PK754_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09397.1"
                     /translation="MKRISVFAVIFTFLFSALPLFSQIGSSSAITDRYGSMFTYSAKT
                     LKFPQYYFYQNNKLFIGTANRGIGASTKVKDYGAQLGLNFGFTESFDVIINGNFVQTP
                     NRASSIPKNKIVGFFNSVDVPDEFYLNLRYIPWVWKDGQYRLGFMLTRKFNGNGFPNA
                     PFQVYQTESPDVGLSLIGSYFAMPGIEDNGLMAHINLQYWNHLDAGKYIAHNDEDDVR
                     EAVNSGGKSLADTSVVESNTSSLRFSLGAQYPLFLGGRYLFLTGDVYGLFYLQKPPVA
                     AYSRQNYAYLALGAKYQAFNWMAVHVGADFRVLSGSDKTISSDLLEINDLTIGKSDYP
                     TWHFYAGVTVPLEPRVRVVRANDESELYARQSKIKKNEVKDILYSEQEIQKRSQNFIP
                     LKEMRRSYKSIIGNLLGVLEPKDKKPVEPEPPAEEQSAEGQN"
     gene            6780..>7840
                     /locus_tag="PK754_07565"
     CDS             6780..>7840
                     /locus_tag="PK754_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNB09398.1"
                     /translation="MSVTIKELNFKQLDKFISFQWKIYADDPNWVPPLRIERKEFLDP
                     GKNPVFGHLDIAFYMAFDEKGEEVGRIAAIVNKNHNTFHEDKVGFFGLFECIHDQNTA
                     TALFDQAFQFLKNKGCDTMRGPVNLSTNDDCGLLVEGLNEPAQIMMTYNPEYYIQLFE
                     KYGFVKSKDLLAYKIDVPDTPPERLKRGAELIQKRGGFTIRTINVKDFKNEVKRIKAI
                     YNSAWERNWGFIPMTDAEFDHLGTQMKQILDPDFLFIAEHNGVPVGFSLTIPNINEAL
                     ITIRDGKLLPFGLIKLLWHTRPGKLQSVRVITLGITPEHRNSGMDTIFYYRSFETAIR
                     KKIKWAEMSWILEDNIPMNR"
     assembly_gap    7841..7940
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7941..8774
                     /locus_tag="PK754_07570"
     CDS             <7941..8774
                     /locus_tag="PK754_07570"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNB09399.1"
                     /translation="GDITDRNSLIDAVRDKDIIFHLGGLTKAKSVTDYYRVNADASQH
                     LYEICLQHNPGIRKIVHVSSLAAAGPSTPGKPRVESDSENPLTDYGKSKLAGEKIAVS
                     FMNKLPITIIRPPAVYGPREKDIFFYFQLIDKHIKPVLGFQTKYLTIIHALDLVEAIV
                     RAAESPQSTGHTYFVDDGYVYSWQDLSSTIQKASGTWAIPLTIPEWLISGIAHIAEFF
                     SRFSSNPALLNRQKIIELKQVAWTTNSEKIKRDLGFSPKYDLLKGCKMTVDWYRENGW
                     L"
     gene            8781..9656
                     /locus_tag="PK754_07575"
     CDS             8781..9656
                     /locus_tag="PK754_07575"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNB09400.1"
                     /translation="MKTFLNKINPVDWAYFIYQAVIIVMIIVFRTNLDQWASYVLWHL
                     LFILAGTSIIHFFHDTPVFALRMFRHWYVILTYAHFFIETGMLNQIVFQGFWDKSFIE
                     WDIRLFGTDPNLWFYDHLNNFWFNEIIHFCYFSYYIVPTALGVILFTQRDTNYFRLLL
                     GFTVTFYVCYFIYILTPVRGPLEMREVRFLDGGWFVQIMNWIYANAEKPGAAFPSSHV
                     AIGLLTLYYAFRYRRNLFWIFLPFMTGLVFSTVYCFYHYVVDVFAGVFVSIVMFWLCE
                     KWYAHSLKSRFPTSI"
     gene            9751..>10341
                     /locus_tag="PK754_07580"
     CDS             9751..>10341
                     /locus_tag="PK754_07580"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNB09401.1"
                     /translation="MRILLVPIIMLLIVSCANNASDKEGWRKIQSGTTDVLLGISMPS
                     QTTGYIVGTNGRILKTTNGGNDWSSQTSGTTSFLRDVYFVNDSLGVAVGESGKILYTD
                     DGGEHWNVKVSNTTSHLVHVHFANSKSGIASGQNGVILYSSNGGDTWLPRVSGTASEL
                     AGCFALSDSDFVVVGFDGTIRSSTNAGRNWVSETSGT"
BASE COUNT         2808 a         2268 c         2139 g         3026 t
ORIGIN      
        1 gattttattg ccgtaggttt aaccggcgct gaatatttgt taaagaaaaa agtcaaattg
       61 gtcggtgttg attatttgag cgtggaaagc tttcacgccg aattcgatca tcccgtgcat
      121 aaaaaattac ttggcagtca cgtggtcgtc gtcgaaggtt taaatctttc ggatgtccgg
      181 cccggtgagt acgaactaat ttgtatgccg cttaaaataa aacacggcga cggaacgccg
      241 gttcgcgcag cgctccgtga tcttcctaat gccaaagtaa agtcaaaacc taaaaagcaa
      301 accgcggcca ataaaaaacg caaaaagaaa taaattttac ataaaaaacc tcggctttaa
      361 aaccgaggtt tttttacaaa tatggaccgg cccccagagt aaacctattc gcgttgacga
      421 attttttact tatagatttc gccggccctc ctcaaattat ttgatggaaa taaaattatc
      481 cgtttgccgg cttatccatt cgaccaaacg acgtgcggat gcggaagagt tttcccacga
      541 tgtttgtgtc caacatgtat ccaagacggc atcgccgctt tcgctataaa ccgttagaca
      601 taaaatcgca tcctgtcggt cggacgtact tactttttca ccgatcatct ctgcaatatc
      661 gcggtccaat ctacgcaaag tatctgcggc aaaaaaaaca accgatgccg ggcctcgtcc
      721 tccttccgta agctgggaca tttgaccatc ataggacagt tctacatagc gtttatagag
      781 acctgctttt tgctctacaa cgatatacca acgcataacc ttaggtgatt cgaccattac
      841 aggtggcgcc tcagcatcag acttagtttc agttgttttc ctgatgtttt tgctaccgga
      901 acaactcaaa taaaacaacg ccaagagtac actcaacaaa aacacacctt gtgattttgt
      961 ttttaataaa aatctatgca tagagcggga aatttttagt aagatccgtg atttcatttt
     1021 ttattttatg gagttctgcc atttggtcgg ggtggcgaat cgcccggtca ataaatccgg
     1081 cgataattcg catttccgat tctttcatac cgcgtgtcgt caacgccggc gtgccgatac
     1141 gaataccgct tgttacaagc gggctttgta catcaaaagg caccatgttt ttatttaccg
     1201 ttatgccggc atgctcaagt gtttgctcgg ctttttttcc ggtaatattt ttattggata
     1261 gatcaaccaa taccaaatga ttgtctgtac cgccggaaac gagtgtataa ttcagattga
     1321 ccagttgctc tgctaatgcc ttcgcattag ccatcacctg acgggcatat tctttgaacg
     1381 aaggctgaag cgcttcttta aacgcaatag ctttcgcggc aatgatgtgc attagcgggc
     1441 caccttgaat accgggcata acattgctat cgaggatttc agacatcatg cgtaaccgac
     1501 cggatttggg agccgtaatt cctaatctgt tttcaaaatc ttttcccatc aggataagcc
     1561 ccgaacgcgg gccgcgcaac gttttgtgcg tcgtcgtcgt tacgatatcg caatacggta
     1621 ccggactggg atgcaagccg gctgcaatga ggcctgccgg atgcgccatg tcggccataa
     1681 gataagctcc aatttcatcc gcaattgcgc gaaatttttc gtattcaaaa aaacgtgtat
     1741 aggcgcttgc accggcaatg atcaatttcg gtttatgctc gcgcgccaca cgtaccattt
     1801 gatcgtaatc aatgcggccc gtttcttttt ttacaccata cgatacgacc ttgtacattt
     1861 ttcctgaaaa atttaccgga ctgccgtgtg tcaaatgacc accatgcgcg agatccatac
     1921 ccatcaccgt atcgccgggt ttgatcaaag taaaatacac agccatgtta gcctggcttc
     1981 cggagtgcgg ctgcacattg gcatacgcgg caccgaaaag ttctttggca cgatcccgag
     2041 ccagattttc ggcgacatct acaaattcac atccaccgta ataacgattt ccgggatagc
     2101 cttccgcata cttattggtc attactgttc cggccgcttc cattacggct ttgctgacaa
     2161 aattctcaga tgctatcatt tcgagcgtcg tattttgacg tactttttca tccgcgatgg
     2221 ctttgtacaa ttcgggatca aactggtgaa tgtgttccat gaagtagaaa tccttttttt
     2281 gttcgtgaca atgtagtgat caagcctatc aaggtgcaaa tgaaaatttt ggccgcttat
     2341 ctcggataag aaacaaaaac actatacgcg tcgggatcaa cttctaatgt ttttaaaatc
     2401 ttatttgtag aaacatcaat aacggtaatc aatcctgttc ctccgctgcc gccgtgatgt
     2461 ggtatgatgg cgccgtcttt atttcggtta ctcacataca cgagcgaccc gtcaggactt
     2521 acggaaacac cgttaggttg agcaaggcga tgaaatgttt taccatcgtc cgaagtctgc
     2581 gcggtaatat ccgtttcctt ataatatcct tccgcattct tttttataac ggtaatactg
     2641 ctatcgttgg agttagccac aaaaactgcg ctcccatcag gtaacacgtc taattggatt
     2701 ggttttgcgt tggtattgtg gccgacatta atggcttgtt taaaagtaaa cgtattaccg
     2761 ttatttgttt tagtacgttg gaaaatacgt acctgcgcat tttttcgaca cgtaataaaa
     2821 agcaaatttt cataaccact gttatttttc ggacccatcc cgatgtgata aggttcgtat
     2881 ttgggcgttg gcgttacatc gtccgccaca tcaaaatgag tagcaagcgt gtcggcttgg
     2941 gtattgatga catagattcg atccgaatac actgcactga catacagcaa agtgccgtct
     3001 ttagaaagat taattccgtg cgggctaacg cccacaggga tagattttat ctccgacatc
     3061 gtttccgtat tcagcacggt gatacgtccg ttgttccctg tattatttac ttcaaatttg
     3121 gtggtcacat acagcttcga tccgtcttgt gaaagtttta ttaacgcaac agggtccagt
     3181 aacgccgcct tgtcaattaa ttcgccggtt ttagcatcgt atttaaaaac atcgccggtt
     3241 gcaatcatgg atgtgtagaa atatttcccg tcacggctca cttcgatccc gtgcgggctt
     3301 tcgttttgtg ggcgatcccc gacatcaaac acgcgcatta tattgtgcgt tcgtgcatcc
     3361 agcacagaaa caatgtcatt aatctggttg gtcgtaaata ttttatttcg aacatgttca
     3421 aaaggaatat tcccttcaag actcggtgca ccatcttcga tccaggattg tattatggta
     3481 acatccgatg cgaccagtgt atctttgttg tacggtgcgt agtttggcgg catacgcgga
     3541 tccataacac cacgaatatg tcccatcata tggcttttgt ccgcacgata cgcgacgact
     3601 gccgcgccgt tttcaccacc ttcaaacaaa ccgctccatg atgataaatc caaattattg
     3661 ggcaggtgaa gagctttggc aagatcatgt tcgtcgggca aatggcatcc tgcgatagcg
     3721 cacgattttt gaaaaatcga atccactgcc accggaaatt cttttatgga catgtgtttt
     3781 ttttcttgcc cgcatgaaat aagaaagaaa atcatacccg ccacaaagat agagtttttc
     3841 attcgttgtc cttcctaaag cactattccc gctgttgtgg cgggaatagt ataaaacacg
     3901 tcatatttga tatgatttta gtttgattac gatagcgctt ctatcactac ggcgacgcct
     3961 tgcccgccac cgatacatgc agaagccaaa ccgtattttt tgccacgacg cttcaattcg
     4021 tataacgcag ttaatgtaag ccgcgcaccc gatgcagcca gcggatgccc gagcgcaata
     4081 gcgcccccgt ttacgttggt aatttcgcgg ttcaggccaa gttctttttc gcaagcgata
     4141 tattgcggcg caaaagcttc attgatttcc acgatgtcca tatcagaaac tttgagcccg
     4201 gcatccgcaa gcgctttttg tgaagaaggc acgggaccga tacccatgat cgaaggatca
     4261 acgccggcgt gaccccagct tatcaggcga cccaacggct ttagcccttt tgctttggct
     4321 atctcagcgg tagtaataag tacggctgca gcaccgtcac aaattccgct ggcattgccc
     4381 gctgatatcg tgccgtcttt tttgaaatac ggtgcaagtg tttgaagcat ttcgactgtc
     4441 gtttccggcc ggggatgttc atccgtgtca aaaaccttta cacctttgcg ggtttttatt
     4501 tcgataggag caatttcatc tttgagtcgt ccgccaagta ttgcgtcctt tgctcgcatt
     4561 tggctaaggt acgcataaag atcacaatcc gtgcgcgaaa tttttaattt atcggctaag
     4621 ttttcggctg tctgcgccat cgaaaaaccg cagtgcggat cggataacgc ttcccacaat
     4681 gaatcttcaa atttcatggg ttgaccaaga ttgactccaa atcgcgtgcc ccgaacaaca
     4741 aacggagctt ggctcatact ttccgtaccg cctgccaaaa ccatcgtgga ttcgcccaaa
     4801 agaatttggc gcgcaccttc gatgattgct tcaaagccgg agccgcaaag gcgattcaaa
     4861 gtcaaagccg gtgtggtttg tggcagtccc gccttgagcg ctatatgacg cgccatatag
     4921 atcgcatctg cactggtttg tgatgcattg ccaaaaacta cgtgatccac atcttcaggt
     4981 ttgacacccg cgcgtttgat ggtctcctgt gcagcaaaaa cgccgagttg gatagcggaa
     5041 aaatctttca gtgttccgcc aaattttcca aacgccgtgc gtgcaccggc gactattaca
     5101 atttctttgt tcaatttcat ctaaaaaact ccgattgaaa gaatgatatt tggttttttt
     5161 gatacgagga aagaatagtg tttttgataa ataaattcca ccccaaaaca aaaaacccgc
     5221 acatttttgt tgtgcgggtt tttggaataa gactaaaaaa ttaattttgt ccttcggcgc
     5281 tttgttcttc ggccggaggt tccggttcaa ccggtttttt atctttgggt tccagcacgc
     5341 caagcaggtt accgataata gacttataac tgcggcgcat ttctttgagt ggaataaaat
     5401 tctgactgcg tttctggatt tcctgctcgg aatacagaat atctttcact tcattttttt
     5461 tgatcttcga ttggcgggcg taaagttcac tctcatcatt ggcccgaacc acgcgaaccc
     5521 gcggttctaa gggcaccgtc acgccggcgt aaaaatgcca agtaggataa tctgacttac
     5581 cgatagtaag atcgttgatc tcaagcagat cgctggaaat agttttatct gaacctgata
     5641 atactcggaa atctgcacca acgtgcacgg ccatccaatt aaacgcttga tattttgccc
     5701 ctaaagcaag gtacgcataa ttttgtcggc tataggctgc taccgggggt ttttgtaaat
     5761 aaaacaatcc ataaacatcc cctgtcagaa aaagataacg accgcctaaa aacagaggat
     5821 attgcgcacc aagcgaaaaa cgaaggctag aggtgtttga ttcgacaacc gatgtatctg
     5881 ccaatgattt accaccgctg tttacagctt cgcgcacatc atcttcatcg ttatgggcaa
     5941 tgtatttacc tgcatccaag tgattccagt actgaaggtt gatatgcgcc atcaatccgt
     6001 tatcttcaat gcccggcatg gcaaaataag aaccgattaa acttaacccg acatccggcg
     6061 attctgtttg atatacctga aatggcgcat tgggaaaacc gttgccatta aacttacgcg
     6121 ttaacataaa accgagacga tattgaccgt ctttccatac ccacggtata tagcgcagat
     6181 ttaaataaaa ctcatccggc acatctactg aattgaaaaa accaacgatt ttattttttg
     6241 gtatacttga tgcacggtta ggtgtttgta caaaattacc attgatgata acgtcgaagg
     6301 actctgtgaa tccgaagttc aaacccagtt gtgcgccgta atctttaact ttggttgatg
     6361 caccgatacc gcgattcgca gttccgataa acaatttatt attctgatag aaataatatt
     6421 gaggaaactt taatgtcttc gcagagtaag taaacatgga cccgtaacga tccgtgattg
     6481 ccgacgaact accgatctga ctaaacagcg gtaacgcaga aaaaaggaaa gtaaaaatga
     6541 ctgcaaaaac agaaatccgt ttcatacaag cgctccgaaa caacatggaa atttgaaagc
     6601 aaagtaaagt aaaataaggg gtttataaaa gcaagcgatt tttaaaaact ggttttatga
     6661 ttttgctcaa cttcattata gaagcgattt cctcaaaacc tactttccgg ttgaaatatc
     6721 ttattgaaag taaaacacac ggtatgtatg tttgcatcat tttcaaaaag aaaagcaaca
     6781 tgtctgtaac catcaaagaa cttaatttca agcagctgga taaatttatt tcgtttcaat
     6841 ggaaaatcta cgccgatgat ccgaattggg ttccgccgct tcgtattgaa cgtaaggagt
     6901 ttctcgaccc agggaaaaat ccggtattcg gccatctcga tatcgctttt tatatggctt
     6961 ttgatgaaaa aggtgaggaa gtcggccgta ttgcagccat tgtaaataaa aatcataata
     7021 catttcacga ggacaaggtt ggtttttttg ggctatttga atgcatccat gatcaaaaca
     7081 cggcaacggc attgtttgat caggcatttc agtttctcaa aaacaagggc tgtgatacca
     7141 tgcggggccc tgttaattta tccactaatg atgattgcgg attattggtc gaaggtttga
     7201 atgaacctgc acaaatcatg atgacataca atcccgaata ttatattcag ctttttgaga
     7261 aatatggttt tgtaaaatcc aaagatttat tggcctacaa aattgatgta ccggatactc
     7321 cgccggaacg acttaaacgc ggtgcggaac tgatacaaaa acgtggtggg tttacgatca
     7381 gaacgattaa cgttaaagat tttaagaacg aagtcaaacg tatcaaagct atttacaatt
     7441 cggcatggga acgtaattgg ggctttattc ccatgacaga tgccgagttt gatcatcttg
     7501 gcacacagat gaagcaaata ttagatccgg attttttatt tatcgccgaa cataacggag
     7561 ttccggtagg cttcagtttg accatcccca atatcaacga agctttgatc acgattcgcg
     7621 acggaaaact tcttccattc ggtttaatca aacttttatg gcatacgcgt cccggcaaat
     7681 tacaatccgt tcgcgttatt accttaggta ttacaccgga acaccgcaac tcgggtatgg
     7741 atacgatttt ttattatcgc tcttttgaaa cggccatccg aaaaaaaatc aaatgggcgg
     7801 aaatgtcctg gattctggaa gataatattc ctatgaatcg nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn ggcgacatca cagatcgtaa ttctttgatt gatgctgtac
     7981 gcgataaaga tattattttt catttgggcg gtctcaccaa agcaaaatcc gtaactgatt
     8041 attaccgtgt caatgcggac gcttcacagc atctttacga aatatgcctt cagcataacc
     8101 ccggaattcg caaaatagtc catgtcagca gtttagccgc ggccggccct tctacacccg
     8161 gcaaaccacg cgtcgaatca gactctgaaa acccgctgac ggactatggt aaaagtaaac
     8221 tggcaggtga aaaaatagca gtatctttta tgaataaact cccgattacc attattcgcc
     8281 caccggctgt gtatggtcca agggaaaaag atattttctt ttattttcaa ctgattgata
     8341 aacacatcaa gcctgtactt ggttttcaaa caaaatatct cacgattatt cacgccttgg
     8401 atttggtaga agctatcgtt cgtgctgccg agtcgcctca aagcaccggc cacacatatt
     8461 tcgttgatga cggatatgta tattcatggc aagacttgag ttcaacgatc caaaaagcct
     8521 ccggtacatg ggccattcct ttgaccatcc cggagtggct gatcagcgga attgctcaca
     8581 tcgcagaatt tttttcacga ttttcttcaa accctgctct tttgaatcgt caaaaaataa
     8641 tcgaactcaa acaagtggcg tggacgacta attccgaaaa aataaaaaga gatttaggtt
     8701 tctctcctaa atacgacctt ttaaagggat gcaagatgac ggttgactgg tatcgtgaaa
     8761 atggatggtt ataataaaaa atgaaaacct ttttaaataa aattaacccg gtggattggg
     8821 cctattttat ttatcaggct gtaatcatcg tgatgattat tgtctttcgg actaatcttg
     8881 atcaatgggc atcttatgtt ttgtggcatt tattatttat tttagccggg acatcaatca
     8941 ttcatttttt tcacgatacg cctgtctttg ctctgcgtat gtttaggcat tggtatgtca
     9001 tcttgactta tgctcatttt tttattgaaa ccggcatgct caaccaaatt gtatttcagg
     9061 ggttttggga taagtcattt attgaatggg atattcgatt gttcggcaca gatcccaatt
     9121 tatggtttta tgatcacttg aataattttt ggttcaacga aatcattcat ttttgctatt
     9181 tttcatatta tatcgttccg acagcgctcg gcgttatttt atttactcag cgcgatacca
     9241 actattttcg tctgttattg ggttttacgg tcacttttta tgtgtgttat tttatataca
     9301 tactcacacc ggtacgcggc cctctggaaa tgcgcgaagt tcggttttta gacggcggat
     9361 ggtttgtaca aattatgaat tggatatacg ccaatgcaga aaaacccgga gctgcatttc
     9421 ccagctccca tgttgcgatc ggtttactta ctttatatta tgcgtttcgc taccggcgaa
     9481 atcttttctg gattttttta ccgtttatga caggattggt tttttctacg gtttattgtt
     9541 tttatcacta cgtcgtagat gtctttgccg gtgtttttgt cagcattgtt atgttttggt
     9601 tatgtgaaaa atggtatgcg cattcgttaa aatcccgttt tcctacgtca atctaacttt
     9661 tttaatcccg ctctatacat taagtgaaac cttccatagt gacgtacgta tcctgattta
     9721 atcctttctc gtcttaccaa aggaaatcct atgcgtatcc ttcttgtacc gattatcatg
     9781 ctattaattg ttagttgtgc aaacaatgca tctgataaag aaggatggcg aaagattcaa
     9841 tcgggcacta ccgacgtgct tctaggaata tccatgcctt cccaaactac ggggtacatt
     9901 gtcggaacaa atggtcgtat tttaaaaaca accaacggcg gaaatgattg gtcatcgcaa
     9961 accagcggta cgacatcatt tctgcgggat gtttattttg taaatgattc gcttggtgta
    10021 gccgtaggtg aatccggaaa aatattatat acagacgacg gcggagaaca ttggaacgtt
    10081 aaagttagta atacgacttc acatttagtg catgttcatt ttgcaaattc aaaatccggt
    10141 attgcatcag gtcaaaatgg tgtgatttta tattcctcca acggaggtga tacttggctg
    10201 ccacgcgtca gcggaaccgc atccgaattg gccggatgtt ttgcattaag tgactccgat
    10261 tttgtcgtgg tcggctttga cggaacgata cgcagcagta cgaatgccgg tcgcaactgg
    10321 gtaagcgaaa caagcggaac a
//
LOCUS       DAOQNQ010000096        10305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2340_length_10305_cov_6.652683, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000096 DAOQNQ010000000
VERSION     DAOQNQ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10305
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2340_length_10305_cov_6.652683"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4490
                     /locus_tag="PK754_07585"
     CDS             <1..4490
                     /locus_tag="PK754_07585"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNB09402.1"
                     /translation="AHGMVHGDIKPENILIESAGDDCKVTLIDFGFIHAWSNPPVSRD
                     HQGTLAYTAPEILKKEPYDQRSDAYALGVVLYELATGINPFADENVVNIVVHHLQKNI
                     THVDSDFEWWSQDYEKIIVSLLSKDPRQRYPRLEDAFKKLTDQNAVLPPLLCHRFAEP
                     RSVWPDISAQLQTDHPTSVVLRAGEGLGATTMLRELYVRAIHSGYSAQYIRLDQAASL
                     WEVLSGFVPASVLETLSDTDRAIEEILKTIAPNEIYILDQVRLDVPGVMDFLSRFTYT
                     LSSNSGSVILSTTELQTEISFPAKTYVLKNLEPQDVKHFLSTWLFDEDVDEKLVSRMT
                     TWTNGHPGLLYEATRELMEKRQLVFEERSWRLTPTAEWKPSSDWSEKWEAKIKALTDD
                     EIRLIRWMAVADNEIPVLTLTALMENAGAEQNIPRLELFGWIRRKENAVKIVAPGLRA
                     FLIQKETEAERRAMHDKLLSWYEKNAADAVPQLAYHAFEGNNQEKSVPYKLQWAEREV
                     SRWNYAEALRQYENVLPLIVDAEKQTEISFAMELLADHLSDRKLQNALLLHIEKQIHS
                     PHPRFVSFIIRRALYLERTGQPDEGVLFCQKHLESDVIKAKQDRARIRRQIGKYKYQK
                     TLFTEARSDFEEAAHMAGETDDITLQMECQNSLGTVYGSLGEYALADKAFREVLRLAR
                     KTEAMASEVSALFNLGQIAFRCGALLQALTWYDEAANLLSLHPNNSQLLRLYLYRASA
                     RIRLRQFEMAFEELQSATAKAMTLNDRTALQRILQSKGVIYQNVGLYELAQSELLQAI
                     EVGQNTNPLNTHIVKLEYSHVLFELGDRVEAHRYCKEAILFFEEKKEDVWLCYAQSVN
                     QILHEYDYSGDETWLQLDALSSKIQDYSVRLFAQQVIAQSLVKKGNYDEALIRLQKVV
                     AEIENQHQYDYSESPIHWLHYRILRTLHKPRYELAKAMDNAYRTMQADLDKLHNPAWR
                     TAYENISLHSTIRAAYEDFFKEERETDIRSFQQLYEITRDINSILDPEKLFEKIMDGA
                     LFNARADRGLILLRNEETGGFDVRVARNVDSDSMADLTTISQSIVDEVYASGASMVTS
                     DANSDMRFKERKSIVAYQIRSILCVPLKLRDHIIGAVYVDKRFDTHYFNSADLKFMEA
                     FAHLAAIAISNAQDYEKVQSENDSLIHENLDLRKAVEERFTRFNIIGKSKAVDQIYRM
                     IEHAAKTDAPVLITGESGTGKELVARAVHHQSLRRRNKFVAVDCGAVTESLLESELFG
                     YKKGAFTGAFTDKKGLFEEADGGTIFLDEITNTTLSFQSRLLRVLQEQEIRRVGDTIP
                     RKIDVRVVAATNKDVRQLITDGQFREDLFFRISVIPVFLAPLRERREDIPLLIKYFVE
                     KYTQPGRMMEGLFGDALQILTDHDWPGNIRELENTVQRLMIFSGKSKLGPSDVRKAMQ
                     PSAAPLKSLEDFEKNLFESERKYFERVLDHVGGNKSKAAEWLGIKRTTLNDRLKKLGI
                     S"
     gene            4504..6228
                     /locus_tag="PK754_07590"
     CDS             4504..6228
                     /locus_tag="PK754_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB09403.1"
                     /translation="MNPTNWPVWAQDMALKIQSKTTSQFILYNNVRDLMALENPKFPG
                     PMDYVAVGDFLAGSVFKERNTVLFYDISKGVYFLDSAMQAEFFKTMQITQQDLMKLPS
                     YALKAIEIYIRLSVEKKRSVAVIIDHAETVIPATLTSAMSLEDRKCLVMLRKWANDPS
                     YIKNNISIVLITDTLNDLNNRLVRNTSTDKVEVAYPDESERIHYIRYFIDEEIREKAP
                     RLFADYASVTPEQIAHTMAGLNRRQLKILLAYARENNKRIDFAYLVAMKKEIIEEECF
                     GMLEIIEPRHDLNAVVGYDAIKSKLRNLAATIKMGKFDAVPMGYLVSGPVGTGKTFIV
                     LSFVGEIGIPCVKFLNFREQWQGVTEANLEKILNLLRAMWPIGVIIDEADAFLGDRDQ
                     QGDSGTSNRIFAQLASFMGDTSYRGKVIWFLITCRPDLLPIDMKRQGRAEEHISVFYP
                     ETSEEKELLFRSMTRRLNAKIDESIHLKDVFDIHQTVSGADMEALVTRAKLQQAIVGA
                     DTVTVEMLRSIFQDFIPTNDPESVELQNLAAVIECTSKSLLPQKYATMDRSEIRRKFD
                     ELLKRYNQ"
     gene            complement(6261..7469)
                     /locus_tag="PK754_07595"
     CDS             complement(6261..7469)
                     /locus_tag="PK754_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNB09404.1"
                     /translation="MIKKFGRAIQWISWGLLTFSFCQCGNSDETTLSKSYPAITAKFG
                     QQIDVKNLANYEDQAIPTYITKDNTGLNTITNEKATVGRVLFYDKNLSFDNSISCAGC
                     HHQSLAFGDTALASRGVQGGLTGRHSMRLVNSRFASETRFFWDERAISLEDQTTRPIK
                     DHAEMGFSGESGRPGFDSLLIKLSAIDYYRELFDLAYGDETITEARLQECMGQFIRSI
                     QSFDTRYDAGRAQVTSDLDNFPNFTTQENQGKTLFITPPVFNASGERTSGGLGCAGCH
                     RAPEFDIDPNSGNNAIIGKLNADGFDVTNTRAPSLRDMTGPDGEPHTPMMHTGVITTL
                     QAAIGHYGTITIGANNTLLDPRLKPNGFGQKLNLTSAEVNAVIAFLKTLSGTQVYTDA
                     RWSDPFGTQP"
     gene            complement(7696..8616)
                     /locus_tag="PK754_07600"
     CDS             complement(7696..8616)
                     /locus_tag="PK754_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09405.1"
                     /translation="MKQEDYSTTNLNLRSEILQISGLSVLILLLLLFLGGCSISGDDF
                     TDTVQDEIKQIIKEDEQLAYGVEVVNDSSEITAPQKSAASESALVELLPRFGRKIESI
                     ERTFQFFPEDFDTTVAVAVTHTLKGYLNIRDSNEVQVSKPLEHSLNRVITFAKRDNKW
                     VHTSISGAFGYSENSKLEMDSLIIRTANDTIVIGNATDFIFYTRRPFSVKRGDSISVT
                     VRAFNKSNLLDRPMGSVLNGQNKTRERTRFNMNAFLGGRYERTITLKPTHKVGFNQLV
                     VDFMTPSSLSININKPYDSFMILIPYFVNE"
     gene            8942..9829
                     /gene="sppA"
                     /locus_tag="PK754_07605"
     CDS             8942..9829
                     /gene="sppA"
                     /locus_tag="PK754_07605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNB09406.1"
                     /translation="MKNRSTWILLLGFLSCFVGLIIYVVYRVAEPDVNFGRRIALIEL
                     EGTIVDSRDIVRQFKKYRDDSSVEAIVFRIESPGGGITPSQEIFDIVKKTKAAGKKII
                     VSMGSVAASGGYYVACPADTIVANPGTITGSIGVIIQFPNVDKLLDKIGVDFTTIKSG
                     AFKDILSPYRPMNPSESEWMQSFVMNAYGQFVRAVADERGLDTAYVQQYADGRVYTGE
                     QALQIKLIDVIGSYDDAIRLAADMAGIEGTPNLVKEKKHKPSFFDLLFEEDAESKIES
                     ALSIPGFKSYPVVSYKYGI"
     gene            9834..10130
                     /locus_tag="PK754_07610"
     CDS             9834..10130
                     /locus_tag="PK754_07610"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNB09407.1"
                     /translation="MATNTTKADIIHRIAEATGITRFDTDAVINAFIAVIHEELQQGR
                     EIEIRGFGSFRIARRPARTARNPKTGAAVDLPERRVPQFRPSKELKKSVNEIRS"
BASE COUNT         2872 a         2214 c         2491 g         2728 t
ORIGIN      
        1 atgcgcacgg catggtgcac ggtgacatca agccggagaa catcctgatc gaatcggccg
       61 gcgacgattg caaggttaca ttgatagact ttggattcat tcacgcctgg tccaatcctc
      121 ctgtttcacg tgaccatcaa ggtacgctgg cttatacggc gccggagata ctcaaaaagg
      181 agccgtacga tcaacgcagc gatgcgtatg ctctgggcgt ggtgttgtat gaactggcca
      241 caggcatcaa cccatttgcg gatgaaaacg ttgttaatat cgtcgtgcat catctgcaaa
      301 aaaacattac gcacgttgac tcggatttcg aatggtggtc gcaggattac gaaaaaatca
      361 tcgtttccct cttgtctaaa gacccgcgcc aacgttatcc tcgtttggaa gatgcattta
      421 agaaattgac ggatcaaaat gcagtattgc ctccgttgtt gtgccaccgt tttgccgaac
      481 cgcggtcggt atggccggat atttcagcgc agctccaaac ggatcatcct acatccgttg
      541 ttcttcgtgc cggtgaggga ctgggcgcga ccactatgct gcgcgaactt tatgtacgag
      601 cgatacattc aggatattct gcacagtata tacgcctgga tcaagcagcg tcgctgtggg
      661 aggtactttc cggttttgtt cctgcatcgg ttttggagac cctgagcgac acggatcgtg
      721 caatcgaaga aattttgaaa actatagcgc ctaatgaaat ttatattttg gatcaagtgc
      781 gtttggatgt acccggtgtg atggattttc tttcgcgatt cacctacacg ctttcctcga
      841 attcgggttc tgtgattcta tcaacgaccg aacttcaaac tgaaatttct tttcctgcaa
      901 aaacatatgt attgaaaaac ttagaacccc aagatgtaaa acatttctta agcacgtggc
      961 tttttgacga agacgttgat gaaaagcttg tttcgcgcat gacgacgtgg acaaacggac
     1021 atcccggctt attatacgaa gctacacgtg aactgatgga aaaaagacaa ctggtttttg
     1081 aagaacgtag ctggcggttg acgcccacag cggaatggaa gccatcttcc gattggtcgg
     1141 aaaaatggga agccaaaatc aaagcgttaa ctgacgacga aattcgtctt atccgatgga
     1201 tggctgtcgc agacaatgaa attcctgttt taactttgac cgcacttatg gaaaacgcag
     1261 gtgccgagca aaatataccg cggttggaat tattcggttg gattcgccgt aaagagaatg
     1321 ctgttaaaat tgttgctccg ggccttcgtg cgtttctgat tcaaaaagaa accgaagcgg
     1381 aacgccgcgc tatgcatgac aaacttttat cgtggtatga aaaaaatgcg gcggatgcgg
     1441 taccgcagtt ggcctaccac gcatttgaag ggaataatca ggagaagtcg gttccgtata
     1501 aactgcaatg ggccgaacgt gaagtgtcgc gttggaatta tgcggaagct ttgcgtcaat
     1561 acgaaaatgt tttgccattg atcgtcgatg cggaaaaaca aactgaaata tcgtttgcga
     1621 tggagttact ggcggatcat ttgtctgacc gtaaattaca aaacgcatta ttgttacaca
     1681 tagaaaaaca aattcactca cctcatccgc gttttgtgtc atttatcata cgtcgtgcgt
     1741 tgtatttgga gcgaacaggt caaccggacg aaggcgtgtt gttttgtcaa aaacatttgg
     1801 aatccgacgt tatcaaggcc aaacaggaca gagcgagaat ccgtcggcaa atcggtaaat
     1861 acaagtatca gaaaacatta tttacggagg cgcgttcgga ttttgaagaa gcggcacaca
     1921 tggccggtga aacagatgat ataacactgc agatggaatg tcagaacagc cttggtacgg
     1981 tatatggttc gctcggcgaa tatgcgcttg cagataaagc gtttcgtgaa gtattgcgtc
     2041 tggcgcgtaa aacggaagcg atggcttccg aagtatcggc cctatttaat ctgggtcaaa
     2101 tagcgtttcg gtgcggtgcg ctgttgcaag ctttgacatg gtacgatgaa gcggctaacc
     2161 ttttatcgct ccatccgaat aacagtcagc tgctccgtct atatctttat cgcgcttctg
     2221 cccgaatacg tttgcggcaa tttgaaatgg cttttgaaga attacaaagc gctacggcaa
     2281 aagcgatgac tttaaatgat cgcaccgctt tgcaacgtat cctgcagtct aaaggtgtca
     2341 tttatcagaa tgtaggtttg tacgaactcg ctcaaagcga attacttcag gcgattgagg
     2401 taggccaaaa tacgaacccg cttaacacgc atattgtcaa acttgaatac agtcatgtac
     2461 tgtttgagct tggagatcgc gtggaagcgc atcgttattg caaagaggcg atattgtttt
     2521 ttgaagaaaa gaaggaagac gtgtggttat gttatgcgca aagtgtaaac cagattctcc
     2581 atgaatacga ttattccggt gatgaaacat ggttgcaact ggatgcattg tcttccaaaa
     2641 ttcaggatta ttccgttcga ttatttgccc agcaagttat tgcacaaagt ttggttaaga
     2701 aaggcaatta cgacgaggcg ctcattcgtc ttcaaaaagt cgtcgcagag attgagaatc
     2761 aacatcaata cgactattcc gaatcgccaa ttcattggct gcattatcga attctcagaa
     2821 cgttgcacaa accccggtat gaattggcta aggccatgga caatgcttat cggacgatgc
     2881 aggcggactt ggacaagctg cataatcccg catggcgcac ggcgtatgag aatatatcgc
     2941 tgcattcgac catccgagcc gcgtacgaag atttttttaa agaagaacgc gagacggata
     3001 ttcggtcgtt tcaacagctt tatgaaatca cgcgcgatat caacagcatc ttggatccgg
     3061 agaaactctt tgaaaaaatc atggacggcg cattgttcaa cgcccgcgcg gatcgcggat
     3121 tgattttatt gcgtaacgaa gaaaccggcg ggtttgacgt gcgtgtcgcc cgcaatgtgg
     3181 attcggattc gatggccgat ctgacgacga tcagtcaaag tatcgtggac gaagtatatg
     3241 cctccggcgc atccatggtc acgtcggatg cgaattccga tatgcggttc aaagaacgaa
     3301 aaagtattgt agcctatcag attcgctcca tcttatgcgt accgctcaag ttgcgcgacc
     3361 atattatcgg tgcggtgtat gtggataaac gttttgacac gcattacttc aattccgccg
     3421 acctcaagtt tatggaagcg tttgcccatt tagcggcgat cgcaatctcc aacgcacaag
     3481 attatgaaaa agtgcagtcc gaaaacgact cgctgataca cgaaaatctg gatttacgca
     3541 aagcggtcga agaacgattt acgcggttta atattatcgg aaaaagcaaa gctgtggatc
     3601 agatataccg tatgatcgag catgcagcta aaacggatgc accggtactg atcacgggcg
     3661 aaagcggtac gggcaaagaa ttagttgccc gggccgttca tcatcaaagt ctgcgtcgtc
     3721 gtaataagtt tgtggccgta gattgcggtg ctgtgacgga atcgcttttg gaaagcgagc
     3781 tttttggtta taaaaaaggc gcgtttaccg gcgcgtttac cgacaaaaaa ggattgtttg
     3841 aagaagccga cggcgggact atttttctcg atgaaattac taatacaacc ttgtcgtttc
     3901 aaagccggtt actgcgcgtt ttacaggagc aggaaattcg ccgcgtgggc gatacgatcc
     3961 ctcgtaaaat agacgtacgc gtcgttgctg ctacaaacaa agatgtacgt caactgatca
     4021 cggacggtca atttcgggag gatttgtttt tccgaatttc tgtcattccg gtatttttgg
     4081 cgcccctgcg tgagcgtcgc gaagacattc cgcttctgat taagtatttt gtcgagaaat
     4141 acacgcagcc ggggcgtatg atggaaggtc ttttcggtga tgcacttcag attttgacgg
     4201 atcatgattg gccgggcaat attcgcgaat tggaaaatac cgtacagcgg ttgatgattt
     4261 tttcgggtaa atccaaactc ggcccttcgg atgttcgaaa agcaatgcaa ccttcagccg
     4321 cgccgttgaa atcattggaa gattttgaaa aaaacttatt cgaatccgaa cgaaaatatt
     4381 tcgaacgcgt tttggatcat gtcggcggca acaaatccaa agccgccgaa tggcttggta
     4441 taaaacgaac gacactcaat gatcggttga aaaaattagg catttcataa aacaagagga
     4501 tacatgaatc caaccaactg gccggtatgg gcgcaagata tggccctaaa aattcaatcg
     4561 aaaacgacat cgcaatttat tttgtacaat aatgtgcggg atctgatggc cttagaaaat
     4621 ccaaaatttc cgggcccaat ggattacgtc gctgtcggtg attttttagc cgggtcggtg
     4681 tttaaagaac gcaacacggt tttgttttac gatatttcaa aaggggtgta ctttctcgac
     4741 agtgcgatgc aggccgaatt ttttaaaacc atgcagatca cgcaacagga tctgatgaaa
     4801 ctgccgagtt atgcactcaa ggccatcgaa atatatatcc gtctcagtgt ggaaaaaaaa
     4861 cgttcggtag ccgtgatcat tgatcacgcg gaaacggtga tcccagcgac gctgacatcg
     4921 gccatgtcac tcgaagatcg aaaatgttta gtgatgctac gcaaatgggc taacgatccc
     4981 agttacatca aaaataatat ttcgatcgta ttaattaccg atacgctcaa cgatctcaat
     5041 aatcgccttg tacgcaatac gtccacggat aaagtagaag tggcataccc cgatgagtcc
     5101 gagcgtattc attatatccg ttattttatt gatgaagaga tacgcgagaa agcgccgagg
     5161 ctttttgccg actatgcgtc cgttacaccg gagcaaatcg cgcatacgat ggccggtctt
     5221 aaccgtcggc aactcaaaat tttattggct tacgctcggg aaaataacaa acgtattgat
     5281 ttcgcctatc tcgtggcgat gaaaaaggaa atcatcgaag aagaatgttt cggcatgctc
     5341 gagatcatcg agccgcgcca tgatctgaac gcggtcgtag gttatgacgc gatcaaaagt
     5401 aaacttcgta atctggcggc cactatcaaa atgggtaaat ttgatgccgt accgatgggt
     5461 tatctggtaa gtggtccggt gggtacggga aaaacattta tcgtgttatc ctttgtcggt
     5521 gagattggca ttccttgtgt caaatttctc aattttcgag agcagtggca gggggtgacg
     5581 gaagcgaact tagaaaaaat actcaatctg cttcgggcta tgtggccgat cggtgtgatc
     5641 attgatgaag cggatgcgtt tctcggagat cgggaccagc agggcgatag cggtacgagc
     5701 aatcggattt ttgcgcaatt agccagcttt atgggtgata cgtcgtaccg cggcaaagtc
     5761 atctggtttt tgatcacatg ccgccctgat cttttgccga tcgatatgaa gcggcaaggt
     5821 cgtgcggaag aacatatcag cgtgttttat ccggaaacat cggaggaaaa agaattattg
     5881 ttccgttcga tgacccggcg gctgaatgca aaaatcgatg aaagcataca tctgaaagat
     5941 gtgtttgata tccatcagac ggtatccggt gccgatatgg aagcgctggt gacacgcgcc
     6001 aaacttcagc aagccatagt cggtgccgac acggttacgg tagaaatgct gcgttcgatt
     6061 tttcaggatt ttattcccac gaacgatccg gaaagtgttg aattgcaaaa cttagccgcc
     6121 gtgattgagt gtaccagcaa aagcctgctg ccgcagaaat acgcaacgat ggatcgcagc
     6181 gaaatccgca gaaaatttga cgaactgctc aaacggtaca atcaataaat tttcattttt
     6241 ccgatcttat ctaaaaaaca ttacggctgc gttccgaaag gatcgctcca acgggcatcg
     6301 gtatatacct gtgtaccgga taacgttttc agaaaagcga tgacggcgtt aacttcagcg
     6361 gaggtcaggt tgagtttctg gccaaatccg ttgggtttta atcgaggatc aaggagtgta
     6421 ttattagcgc caatggtgat cgttccataa tgcccgatag ccgcttgtag tgtcgtgatc
     6481 acaccggtgt gcatcatcgg tgtatggggt tcgccgtccg gcccggtcat atcacgcagc
     6541 gagggtgcgc gtgtattggt cacatcaaaa ccgtcggcat tgagctttcc gataatggca
     6601 ttgtttccgc tattgggatc aatatcaaat tccggcgctc tgtgacaccc cgcacagccg
     6661 agcccgccgc ttgtgcgctc cccggaggca ttaaaaacag gtggtgtgat aaaaagcgtt
     6721 ttgccctggt tttcctgcgt tgtaaaatta ggaaaattat ccagatcgga agtcacctgt
     6781 gcgcgccctg cgtcgtaacg cgtatcaaaa gattggatgc tgcgaataaa ttgccccata
     6841 cattcctgca aacgtgcttc cgttatagtt tcgtcaccat aggccaaatc aaacaattca
     6901 cgataataat ctatcgccga taatttaatt aataacgaat caaagcctgg tcgaccgctt
     6961 tcaccgctga agcccatctc tgcatgatct ttgatcggtc gtgttgtttg atcttcaagg
     7021 ctgatcgcac gttcatccca aaaaaaacgg gtttcggatg cgaaacgtga attgaccaaa
     7081 cgcatggaat gccggcctgt caatccgccc tgaacaccgc gactggccag cgccgtatca
     7141 ccgaaagcga gcgactgatg atggcagccg gcgcaggaaa tcgaattatc aaaactgaga
     7201 tttttatcgt aaaaaagtac ccttcccacc gttgcctttt cgttggtaat tgtattaagg
     7261 ccggtgttgt ctttggtaat ataggtagga atcgcttggt cttcataatt ggccagattc
     7321 tttacatcaa tttgctgacc gaatttcgcc gtaatggccg ggtaagattt agataatgtc
     7381 gtctcatcag aattaccgca ttggcaaaaa ctaaatgtca aaagtcccca actgatccat
     7441 tgaatggcgc gaccaaattt tttaatcata ataattttgt tgcagctttc tgcgtttttt
     7501 aaaagtgcac tgtgaatcaa agatattaac ggtctcataa ttgtaacaac attgtcattc
     7561 aatcaacttc ataatggcga acacaacctc tcgtttctca ctgggttccc gctttcgcgg
     7621 gaacgacaga actgcctcac ccgcgcaagc ggatgtctat acaaatatga ggcgcttata
     7681 tgttcgtttg cgattttatt cattaacaaa atatgggatc aagatcataa acgaatcgta
     7741 tggcttattg atgttgatac tgagcgaaga cggtgtcata aagtcaacta caagctggtt
     7801 aaaaccgact ttatgtgttg gtttgagagt tatggttcgc tcgtaacgac ctcctaagaa
     7861 cgcattcata ttaaaacgtg tacgttcacg ggttttattt tgcccgttaa gcaccgaacc
     7921 catcggacga tccagtaaat tagatttatt gaatgcgcgc acggtaacgc tgatcgagtc
     7981 gccgcgtttg acgctaaacg gacgacgtgt ataaaatata aaatcggtgg cattgccgat
     8041 cacgatggtg tcattagccg ttctaataat aagcgaatcc atttctaatt ttgaattctc
     8101 tgaatatccg aaggcgccgg aaatgctcgt gtgcacccat ttgttatcgc gtttggcaaa
     8161 agtaataaca cggttcagag aatgttcgag aggcttactg acctgtacct cattggaatc
     8221 ccggatattc agataacctt tcaacgtatg ggtaaccgct actgcgacgg tggtgtcaaa
     8281 atcttcaggg aaaaattgga atgtacgttc gatactttcg atcttacgtc cgaaacgcgg
     8341 aagcaattcc accaaagcgc tctccgaagc ggcgctcttt tgtggcgcgg tgatttccga
     8401 ggaatcgttg accacctcta cgccgtaagc caattgttca tcctctttga tgatttgttt
     8461 gatttcatcc tggacggtat ctgtaaaatc gtctccacta atgctgcatc cgcctaaaaa
     8521 caacagcaaa agcagtatca gtacggataa tcccgagatt tgcagaatct cactgcgtaa
     8581 atttaaattg gtagtagaat aatcttcttg tttcataaga acacctccgg cccgaatata
     8641 tatcaaaata catgccgcat ttaattattt gttttacatt actttaatta tcgaatcaac
     8701 ggatttttga cgggaaaacc gtctatgtag tttaacttaa aacgcaagca tatgcaattg
     8761 ctacatatat aagcagattg attatatgtt tttttcaaat ttttcatgaa aagcatgtct
     8821 ttgaaagtat ttacaatatc aattgacagt agccttgaaa atgatatatt tacacgctgt
     8881 aatgggtata gttcggttaa gtaacattga attaaaaaat taagtataca aaaatagaag
     8941 tatgaaaaat cgttcgacat ggattttgct tttgggtttt ttgtcgtgtt ttgtcgggct
     9001 gatcatttac gtcgtatatc gcgttgcgga gccggatgta aatttcggtc ggcgcatcgc
     9061 attgatcgaa ctcgaaggta cgatcgttga ttcgcgcgat atagtgcgcc agttcaaaaa
     9121 ataccgtgat gacagctccg tcgaagcgat tgtatttcgc attgagagcc ccggcggcgg
     9181 tatcacgcct tcgcaggaaa tttttgatat cgtcaaaaaa acaaaagcgg ccggaaaaaa
     9241 aatcatcgtt tcgatgggta gcgtagcagc gtccggcgga tattatgtcg cgtgtccggc
     9301 ggatacgatc gtcgctaatc ccggcacgat cacgggcagt atcggcgtga tcattcagtt
     9361 ccccaatgtg gataaacttt tggataaaat cggtgtggat tttacgacga tcaaaagcgg
     9421 tgcgttcaaa gatattttga gcccgtatcg cccgatgaat ccgtcggaat ccgagtggat
     9481 gcaatcgttt gtgatgaatg cgtacggaca atttgtacgt gccgtcgcgg atgaacgcgg
     9541 cttggatacg gcgtatgtac aacaatatgc ggacggacgt gtgtatacgg gtgagcaggc
     9601 gttgcaaatc aagctgattg atgtgatcgg ttcgtacgac gatgcgatcc gtctggcggc
     9661 ggacatggca ggcatcgaag gtacgccgaa cctcgtcaaa gagaaaaaac acaagccttc
     9721 gttttttgat ctgttgtttg aagaagatgc agagagtaaa atcgaatcgg cgctgagtat
     9781 tccgggtttc aaaagttacc cggtggtgag ttataaatac ggcatctgag tatatggcaa
     9841 ccaatacgac gaaagcggat atcatccatc gcatcgcgga ggccacgggc attacgcgat
     9901 ttgataccga tgcggttatc aatgcgttta tcgccgtgat ccatgaagaa ctgcagcaag
     9961 gccgtgaaat cgaaatccgc gggtttggca gctttcgtat tgctcgacgc ccggcgcgta
    10021 cggcacgcaa ccctaaaacc ggtgcggcgg tagatttacc ggagcgccgt gtaccgcaat
    10081 ttaggccatc gaaagaattg aaaaaatcgg tcaacgaaat ccgatcataa catataacac
    10141 acaaaaaaaa gaaaggaact aaccatgccc tgcggaaaaa aacgcaaaag aagaaaaatg
    10201 gctactcaca aacgtaaaaa acgtcttcgt cgcgaccgtc ataagaaaaa aggtcgttag
    10261 tcttttgatt ttttagtcat tcgtttgtac tataatcccg ccgat
//
LOCUS       DAOQNQ010000097        10201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2377_length_10201_cov_5.697713, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000097 DAOQNQ010000000
VERSION     DAOQNQ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10201
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2377_length_10201_cov_5.697713"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="PK754_07615"
     CDS             complement(<1..920)
                     /locus_tag="PK754_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009783027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB09408.1"
                     /translation="MSANDEFALNVEKAVTERYTEGAQTTVAALCCPVSYDPKYLQIL
                     PQEILDRDYGCGDPSRYVRPGETVLDLGSGGGKICYIAAQIVGSEGRVLGVDMNSEML
                     ALARKYKDPIGQKLGYTNVTFHRGKIQDLKLDWDVLDAYVRENPIQHGDDLQRLELFT
                     QELRNQRPMISDNSVDVVLSNCVLNLVREEDRQNLIAEIYRVLKPGGRFAISDIVSDE
                     DIPLQQKNNPELWSGCISGAFREDEFLKVFEKAGFYGISLDKWETTPWQVVEGIEYRS
                     VTVIGYKGKEGPCLERQQAVIYKGPWKAVI"
     gene            complement(938..2053)
                     /locus_tag="PK754_07620"
     CDS             complement(938..2053)
                     /locus_tag="PK754_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNB09409.1"
                     /translation="MAMLILLFAGAFFVAYTNGANDNFKGVATLYGSKTLGYKASIGF
                     ATVATFAGSVCSIFIAQSLVSNFSGKGLVPDTLTVAPEFLTAVALGAGLTVLIATFFG
                     FPISTTHGLTGALTGAGFLAVGPQLNTEKLGSAFFIPLLVSPLVSVVVAVIVYVVFRQ
                     IRIWAGIREEHCICVGTCQTVIPTPRPAHALSFQSVTLPEITFGDEASCQRRYGGAVM
                     GISAQRMVDALHYISAAIVSFARGLNDTPKIVGLVFAAQIIRIEWSMIAIAIAMAIGG
                     LLNARKVARTMSENITSMNHGQGLTANLVTGFLVIFASRWGLPVSTTHVSVGAISGIG
                     IVTRQADFKVIRQVVLSWALTLPVAAVIAASIYYLIQ"
     gene            complement(2092..3108)
                     /gene="arsS"
                     /locus_tag="PK754_07625"
     CDS             complement(2092..3108)
                     /gene="arsS"
                     /locus_tag="PK754_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560827.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HNB09410.1"
                     /translation="MQLPQILPISPADMKYSFHEKLREHELTLPPLGIHTLQVNITKL
                     CNQACRHCHVDASPKRTEQMDRLTVEHCLAILAAHDEIKNLDITGGAPELNPHFEFFV
                     TEARRLGKHVMVRHNLTVSLDPHPLTKQPMTHLPQFFADHGIEVISSLPFYEEYFTDK
                     QRGRGVFNKSIAALKNLNALGYGKDDSGLVLNLVYNPVGTYLPAAQGALEADYKRSLH
                     DKFGIHFNNLFTITNMPIHRFREDLERTGKYDEYMMKLINAFNPGAAQGVMCRTLISV
                     SYDGKIYDCDFNQMLGMQSHDHEPLSVFNFDATRIRNRAILFASHCFGCTAGSGSSCG
                     GTTA"
     gene            complement(3114..3446)
                     /locus_tag="PK754_07630"
     CDS             complement(3114..3446)
                     /locus_tag="PK754_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HNB09411.1"
                     /translation="MNYYESEDLKKFGDVGRFRKELMDKFFAYYNEVTGKDGALTKRE
                     KALIALAVAHAKNCPYCIDAYTTQSLECGASPDQMTEAVHVASAMMAGITLVHGVQML
                     NNLDNLNV"
     gene            complement(3545..4717)
                     /locus_tag="PK754_07635"
     CDS             complement(3545..4717)
                     /locus_tag="PK754_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HNB09412.1"
                     /translation="MKSFQILLPVVLIAAFLAGSGCADLIEPLDSIDGNDTPETAILF
                     PRLPQVQQSTVSVSDDIDYFRFVLSSPRHVAVTLAVPDDKDYNLYLMDSRDSVVGLSR
                     GANGITESIDIPHLNAGTYFIKILSENGAYSETQLYRLSIADADTGQTPNLHLSLGNP
                     SQSETDITSPRNYLIARSQYAVSYDNIRRIPNWCAWHLSKNWLGTIARQDNFKTDVLP
                     SGWYAVKDGDYTGSGYDRGHMCPSGDRNAVPSDNVSTFYMTNILPQTSDNNQGPWVEL
                     ESYCRDLAGEDYELFIMSGHYDSLKTIASGKVTVPVRVWKIIVVLPHEETTPDQVTTL
                     TRVIAVDMPNTDGIRSTSWKSYRTSVDQIETSTGYDFLNRVPAEIQSVIESQVDNK"
     gene            4880..5524
                     /locus_tag="PK754_07640"
     CDS             4880..5524
                     /locus_tag="PK754_07640"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNB09413.1"
                     /translation="MRVDRIIEAFLPREDKFFKLFEETAQHLITASDLMVKLMNCPAE
                     ERLAVSQEIHKLEHLCDDTAHRLFSELNATFVTPFDREDIMMLASALDDVMDFLDESA
                     RRIILYKITSIPANMKQLADVLKESITETHHGVSHIRNIHKTEGIRKTLERIHACENK
                     ADMVFEMGIADLFDNEKDPINLVKLKDIYTSLERSTDKCEDVANVLEAILIKHA"
     gene            5538..6533
                     /locus_tag="PK754_07645"
     CDS             5538..6533
                     /locus_tag="PK754_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNB09414.1"
                     /translation="MTELLFITIALALIFDFINGFHDAANSIATVVSTRVLSPFVAVL
                     WAAAFNFLAYWLFELHVADTVAKTVKPEAITLVVVASGLVGAIIWNLLTWWLGIPSSS
                     SHTLMGGFAGAAIAYSHGDFSVVRMEKISPVLYFIFLAPVIGMIVSYIISVITIHICK
                     RQSPHKVDKIFRRLQLLSSAAFSLGHGGNDAQKVMGIIAAAMLSQGIIADMHHIPEWV
                     PLACYGSIALGTMMGGWRIVKTMGQKVTKLSPFEGFAAETAGAVTLFGTGYTGIPVST
                     THTITGSIIGVGVIKRVSAVRWGVTRNLLWAWVLTIPISGLFGAICYWLASFFVS"
     gene            6683..8386
                     /locus_tag="PK754_07650"
     CDS             6683..8386
                     /locus_tag="PK754_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNB09415.1"
                     /translation="MKNRRNISYLALIGITIFLYGCSSKSQRTTPPKPPRLIVFIALD
                     QFRYDYLTRFSTHFGANGFNYLLNGGASFANCLYEQATTHTSVGHATMMSGTLPYRHG
                     IIANYWWSRELHRYIYAPEDTLAPILGIEKQKATDGRSPRNFSGINFADALREKTNGR
                     AKVFSVSNKDRSAVLMGGKNPNGAYWMVKNRGGFYTSAYYMNDYPVWVKEYNAQKPAD
                     KYFGMSWEMLLDKEKYPTMKPEILRLLDLPSSGFGHSFPHPLKGLTSDRPDTMYYKEL
                     IHTPFASEMVLDFAKAIVQNEALGSDEITDILCISLSANDEIGHSFGPSSPEVMDITV
                     QTDRLLARFFKYINTAVKEEDVLYVLTSDHGVPFFPEEMADRGVTAARVHPRKLAAVA
                     DSIMALKYGKLPLGKQYVDRIAYPDLYFDGRVLAEKKIDKEEASDYISAVFEQSLPII
                     YRAYSGTRLKRGLVPSDPISEAVRKSFHSRLSGDVVIVLKPFHVWDSNGLGTEHSLPY
                     AYDAQVPLIFKGKRWIKSGLYKDEVAPTDIVPVLSVITGCEDPGGRDGQVPDQLLKFS
                     K"
     gene            8416..8781
                     /locus_tag="PK754_07655"
     CDS             8416..8781
                     /locus_tag="PK754_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB09416.1"
                     /translation="MPGFLTTIKYVCIYASDFDRSLAFYRDGLGLQVTYKEDGFAQFN
                     TEGTILTLEKDGFRSDEPKDYRKVGFLIQFEVEDIEKAVTALKAKNVKFTQTITDKEF
                     GRTAIIVDPDGNQIMLLEQ"
     gene            8840..9814
                     /locus_tag="PK754_07660"
     CDS             8840..9814
                     /locus_tag="PK754_07660"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017248037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HNB09417.1"
                     /translation="MANITYLEAISMGLREEMQRDGNVFLIGEDIGVYGGAFKVTKGF
                     IDEFGAERVMDTPISEAGIIGVCVGSALMGMRPVAEMQFADFISCGFNQLVNQAAKIH
                     YRWGEAVPMVVRCPSGGGVHGGPFHSQNPEAWFFHVPGLKVVSPSTPYDAKGLIKAAI
                     RDNNPVLYFEHKFLYRRIKGEVPEDDYIVPIGKGDIKREGKDMTVVAYSSAVHWALEA
                     AEIIEKEGVSVEVLDLRSILPYDKELILQSVRKTNRVIVAHEATLTGGVGGDIAAFIT
                     EHAFESLDAPVKRLAAIDTPVPYSPPLEAYFLPNKDKMLAMMRELAAY"
     gene            9824..>10201
                     /locus_tag="PK754_07665"
     CDS             9824..>10201
                     /locus_tag="PK754_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09418.1"
                     /translation="MQKRWFNIFSIISLVLFGAGDFILKASDKGEGQELHANRFLADA
                     LEDARHNYAGRTIPASAVVKFEVYRGFLADINKDGESEYIVAGTITGLDINYVMVYLW
                     KNEKWLCTVLNSGLGGGIADLQLL"
BASE COUNT         2700 a         2238 c         2504 g         2759 t
ORIGIN      
        1 tctataacgg ctttccaggg acctttgtaa atcacggctt gttggcgttc aaggcaagga
       61 ccttcttttc ctttgtaacc gatcacggtc actgaacggt attcgatgcc ttccactact
      121 tgccatggcg tggtttccca tttgtctaaa ctaataccgt agaaccccgc tttttcaaag
      181 actttcaaaa attcatcttc gcgaaatgca ccggatatac atccgctcca aagttcagga
      241 ttattttttt gttgaagcgg tatgtcctca tcagacacaa tatccgatat ggcaaaacgg
      301 ccgcccggtt ttaaaacgcg atatatttcg gcgatgagat tttgacggtc ttcttcccga
      361 acaagattga gcacacaatt ggacaaaacg acgtcaacgg aattatcaga aatcatcggt
      421 cgttggttgc gaagttcttg agtaaacaac tccaaacgtt gcaaatcatc gccgtgttgg
      481 ataggatttt cacgcacata agcgtcgagc acgtcccaat ccaatttcaa atcctgtatt
      541 tttccgcgat gaaatgtaac attggtataa cccagttttt gtccgatagg atctttgtac
      601 ttgcgggcta aggctaacat ttcggaattc atatccacgc cgagcacacg cccttcggaa
      661 cctacgattt gcgcggcgat ataacatatt tttccgccgc cgcttcccaa gtccaatacc
      721 gtttctccgg ggcgcacata acgcgagggg tcgccgcagc catagtcacg atcaaggatt
      781 tcctgtggta aaatttgaag atattttgga tcgtaggaaa caggacaaca taaagcggca
      841 acggttgttt gcgcaccttc ggtatatcgt tcggtgacgg ccttttctac attgagtgca
      901 aattcgtcgt tagcagacat gcttaactct ccaaaagtta ttgaatcaaa taataaatgc
      961 ttgccgcgat gacagcggca acgggtaatg tcagcgccca ggaaagaacg acttgtcgta
     1021 tcactttgaa atcggcctga cgggtcacaa tgcctattcc ggaaatagcg cctacggata
     1081 catgggtggt ggatacaggt aaaccccacc gacttgcaaa aataacaaga aatcccgtaa
     1141 caagattggc tgtcagtccc tgaccgtggt tcatggatgt gatgttttcg ctcatggtac
     1201 gcgctacttt gcgggcattg agtaaaccgc cgatggccat agctattgcg atggctatca
     1261 tcgaccactc gatacgtata atctgcgcgg caaatacgag cccaacgatt ttaggcgtat
     1321 cgttgagtcc gcgtgcaaaa ctgacaatag ccgcgctgat ataatgtagc gcgtccacca
     1381 tacgttgtgc cgaaatgccc attactgcgc caccgtatcg cctttgacag gaagcttcat
     1441 cgccgaaagt aatttcaggc agagtcactg actgaaacga aagagcgtgc gctggtcgag
     1501 gggtaggtat gacggtttgg catgttccaa cgcaaatgca atgttcttct cgaataccag
     1561 cccaaatccg aatttggcga aatacaacat aaacgatcac ggcaacaacc acggaaacca
     1621 aagggctgac taatagaggt ataaaaaagg ctgagcctaa tttttcggta ttcaattggg
     1681 gacctacagc aaggaaacca gcgccggtaa gcgcaccggt gagaccgtgt gtcgtcgaaa
     1741 tgggaaatcc aaaaaatgtc gcgatgagaa ccgttaaacc ggcgcccagg gctactgcag
     1801 ttaaaaattc gggggcaacc gtcaatgtat ccggcacaag ccctttgccc gaaaaattac
     1861 tcacgagtga ttgagcaata aaaatcgaac ataccgaacc cgcaaaggtg gcgacggtcg
     1921 cgaacccgat cgaggcttta tatcctaagg ttttactgcc gtagagcgtt gcgacacctt
     1981 taaaattatc atttgcaccg ttggtgtatg cgacaaaaaa cgctccggca aaaagcagta
     2041 tgagcatcgc catttttatc ttctaattaa tttcaggtta tttattttga attatgccgt
     2101 tgtcccaccg caactgctgc ccgaccccgc cgtacacccg aaacagtgcg atgcaaaaag
     2161 gatagcgcga tttcgtattc tggtcgcatc aaaattgaaa acgctaagcg gttcgtgatc
     2221 atgagattgc atgccgagca tttggttgaa atcgcaatcg tagatttttc cgtcataact
     2281 cacgctgatc agggttcggc acatgacacc ttgcgccgcg ccgggattga aagcatttat
     2341 tagcttcatc atatattcgt catatttacc cgtacgctca agatcttccc gaaagcgatg
     2401 gatcggcata ttggtgatcg taaatagatt attgaagtga attccgaatt tgtcatgcaa
     2461 actgcgttta tagtccgcct ccaacgctcc ttgagcagcg ggcaggtatg taccgacggg
     2521 attgtacact aagttgagta caaggcctga atcgtctttt ccatagccca gcgcattgag
     2581 attttttaac gccgcgatgc ttttattgaa aacgccccgt ccgcgttgct tatccgtaaa
     2641 atattcctca taaaaaggta gcgatgaaat cacttcaatc ccgtgatcgg cgaaaaactg
     2701 cggcaggtgc gtcatcggtt gtttggtcag aggatgaggg tccagcgata cggtgaggtt
     2761 atgccgaacc atgacgtgtt ttcccaatcg tcgtgcctcg gtaacaaaaa actcaaaatg
     2821 cggattaagt tccggggcgc cgccggtaat atcgaggttt tttatttcgt catgcgccgc
     2881 aagtatcgct aaacagtgtt ctacggtaag gcgatccatt tgctcggtgc gcttgggtga
     2941 agcgtccacg tggcaatgac ggcaggcctg attgcacagt ttggttatat tgacttgcaa
     3001 cgtatgaatg ccgagcggcg gtaacgtcaa ctcatgctca cgcagttttt catgaaaaga
     3061 atatttcata tcggccggag atatcggtaa tatttgaggc agttgcataa tttctatacg
     3121 ttgaggttat ctaaattatt cagcatttgt acaccgtgta cgagcgtgat gccggccatc
     3181 atggcggagg cgacgtgtac cgcttcggtc atttggtcgg ggcttgcgcc gcattcgagg
     3241 ctctgcgtcg tataggcgtc aatacaataa ggacagtttt tagcatgggc tacagccaaa
     3301 gcgatcaggg ctttttcccg tttggttagt gcgccgtctt tgccggtaac ctcattgtaa
     3361 tatgcaaaaa atttatccat cagttctttg cgaaatctcc ctacgtcgcc aaatttttta
     3421 aggtcttcac tttcgtaata attcatcttg aggctcccat gttgtaagaa tatggatgaa
     3481 gaaaaggatt aattttatga agactgaatg atgtaactcg gaggcaaaac aaatattccc
     3541 atgtttattt gttatccacc tgcgattcga tgacggattg gatttctgca ggtacgcgat
     3601 tgaggaaatc atatccggtg gaggtttcga tttgatccac ggatgttcgg tacgatttcc
     3661 atgacgtgct gcgaatgcca tcggtattgg gcatatctac agcgatcacg cgtgtcagcg
     3721 tagtgacttg atcgggtgtg gtttcctcgt gaggaagtac aacaatgatt ttccatacgc
     3781 gtacggggac ggtgactttg ccggaagcta tggttttgag agaatcataa tgccctgaca
     3841 tgataaaaag ttcgtaatct tcaccggcca aatcacggca ataactttcg agttcgaccc
     3901 aaggcccttg gttattatcc gaagtctgag gaagtatatt ggtcatgtaa aatgtagata
     3961 cattatcgga gggaactgca ttgcgatcgc cggaggggca catgtgtcct cgatcataac
     4021 cgctgccggt gtagtcgccg tctttcactg cataccaacc cgacggcaaa acatccgttt
     4081 taaagttatc ttgacgtgct atcgtaccga gccaattttt actcaagtgc caagcgcacc
     4141 agttggggat gcggcgaata ttgtcataag acaccgcgta ttgagagcga gcgatcagat
     4201 agttacgcgg tgaagtaata tcagtttcgc tttgactcgg attgccaagg gaaagatgca
     4261 aattcggcgt ttggccggta tcggcgtcag ctatggatag tcggtatagt tgtgtctcgc
     4321 tgtaggcgcc gttttcactc aggattttta tgaagtacgt gcctgcgttt aagtggggaa
     4381 tatcaatgga ttctgtgatg ccattggcgc cacgggataa acctacaacg gaatcgcggc
     4441 tatccatgag atagagatta taatctttat catccggaac ggccaaagta accgcgacat
     4501 ggcgcggcga acttaataca aaacgaaaat aatctatatc gtcgctgaca gaaacggtgc
     4561 tttgctgaac ttggggaagt ctcggaaata aaatcgccgt ttcaggcgta tcattgccat
     4621 caatggaatc taacggctcg attaaatccg cacaccccga cccggctaaa aatgcagcga
     4681 tcaatacaac cggtaacaag atttggaagg atttcatata aaaagataaa aaaacttatg
     4741 attcgtccaa agaatttttc aaagcttttc ttgtttctta gtttcaaaat tattatgata
     4801 gcccgcctta ataatccgct aatattcaca taacaaaatt tacacttcga taattcaaat
     4861 ttaataacga ggaaacacta tgagggtaga tcgtatcatc gaggcgtttt taccacgtga
     4921 agacaaattt tttaaacttt ttgaagaaac agcccagcac ctgatcacgg catcggatct
     4981 gatggtcaaa ctgatgaatt gtccggcaga ggagcgttta gccgtaagtc aggaaattca
     5041 caaattagaa cacttatgcg acgatacggc gcatcggctt ttttccgaac tgaacgccac
     5101 atttgtaacg ccgtttgatc gcgaagatat tatgatgctg gcttcggcgc tggacgacgt
     5161 catggatttt ttggatgaaa gcgcacgtcg catcatattg tataaaatca cttccattcc
     5221 ggccaatatg aaacaattgg ccgatgtgct caaagaatct atcaccgaga cacatcacgg
     5281 cgtgagccat attcggaata tccataagac ggagggtatt cgtaaaactc tggaacgtat
     5341 ccacgcctgt gaaaacaaag cggatatggt ttttgaaatg ggaatagcgg acctttttga
     5401 taatgaaaaa gacccgatca atttggtcaa actgaaagat atttatacaa gcctcgaacg
     5461 ctccacggac aaatgcgaag atgtcgccaa tgttttggaa gctatcctca tcaagcacgc
     5521 ttaaccggag aattgcgatg acggaactgc ttttcatcac tatagcgtta gctctcattt
     5581 ttgattttat caacgggttt catgatgcgg cgaattcgat agccaccgta gtttcgacgc
     5641 gtgtgctttc gccgtttgta gccgtgctat gggctgcagc ttttaatttt ctggcttatt
     5701 ggctttttga attgcatgtc gccgataccg tagctaagac ggtaaaacct gaagctatta
     5761 cattggttgt ggttgcatca ggactggtcg gtgccatcat atggaattta ttaacctggt
     5821 ggctaggtat tccatccagt tcatcgcata cgctgatggg cggttttgcg ggtgcggcga
     5881 tagcctatag ccatggtgat ttttcggtcg tgcgtatgga aaaaatttca ccggttttgt
     5941 atttcatttt tttagctccg gtcataggga tgatagtgtc ttacattatt tcagtcatta
     6001 ctattcatat ctgcaaacgt cagagtccgc ataaagtaga taaaattttc cgccgcttac
     6061 aattattgtc ctctgcggct ttcagcttag gtcacggtgg aaatgatgcg cagaaagtga
     6121 tgggtattat tgcggcagcg atgttatctc agggtataat tgccgacatg catcatattc
     6181 cggaatgggt acccctggca tgttatgggt ccatagcgtt gggaacgatg atggggggat
     6241 ggcgtattgt aaaaacgatg ggacaaaaag taactaaact ttcgccattt gaaggttttg
     6301 ctgcggaaac ggccggtgct gtgacattat ttggaacagg atataccggt attcccgtaa
     6361 gtacgacgca cacgattacc ggctcgatca tcggcgtcgg cgtgatcaaa cgcgtatccg
     6421 ctgttcgctg gggcgtaact cgcaatctgc tttgggcatg ggtattaacc attccgattt
     6481 cagggttgtt tggcgcgatt tgttattggc tcgcttcgtt ttttgtttca taaaacgtcg
     6541 gattacaatt tttttattcg ataaaaggtt gctcataaat gagcagcctt tttttttgga
     6601 tttctttgtg cttttctttt ttaatcgtga tatgtatcat gggttatgaa tgaatcgggg
     6661 actacatgaa tttgaggtat atatgaaaaa cagaagaaat atttcatatt tggctttgat
     6721 aggtattacc attttccttt acggttgttc atccaaatct caacgaacga ccccgcccaa
     6781 gccgccgcgt cttattgttt ttatcgcatt ggatcagttt cgctatgact atctgacgcg
     6841 gttcagtacg cattttgggg cgaacggatt taattacctc ctcaatggcg gagcaagttt
     6901 tgccaactgc ttgtatgaac aagccacgac gcacacatcg gtggggcacg ccacgatgat
     6961 gagcggtacg ttgccgtatc ggcacggtat cattgccaac tactggtgga gtcgcgaact
     7021 gcatcgctat atatatgcac cggaagatac gctggcgccg atactgggaa ttgaaaaaca
     7081 aaaagcaaca gatgggcgtt cgccgcgcaa ttttagcgga atcaattttg ccgatgcctt
     7141 acgtgaaaaa acgaacggcc gggctaaagt gttttccgtg tccaataagg atcgctccgc
     7201 cgtattgatg ggtgggaaaa atcccaatgg ggcgtattgg atggtaaaaa accgcggagg
     7261 gttttatacc agcgcgtatt atatgaatga ctacccggtg tgggtaaagg aatacaatgc
     7321 tcaaaaaccg gcggacaaat attttggcat gtcgtgggaa atgctgctgg ataaagaaaa
     7381 atatccaacc atgaaaccgg agatacttcg tttactggat ttgccgagtt caggttttgg
     7441 gcatagtttc ccgcatccgc tcaaaggatt gacgtcggat aggcctgata cgatgtatta
     7501 caaagaactg atccatacgc cgtttgccag tgaaatggtt ttggattttg caaaggcgat
     7561 tgttcaaaat gaagctttag gttcggacga gattacagat atcctttgta tcagtttatc
     7621 ggccaacgac gaaatagggc acagcttcgg gccatccagt ccggaggtga tggatattac
     7681 ggtacagacc gatcgcttgc tggcacgttt ttttaagtat atcaatacgg ccgtcaaaga
     7741 agaagacgtt ctgtatgtgt tgacatccga tcatggggtt ccttttttcc cggaagaaat
     7801 ggccgaccga ggtgtgacag ccgcacgggt tcatccccga aaattggccg ctgtggccga
     7861 ttcgatcatg gctttgaagt atggaaaatt gcctctggga aaacaatacg ttgatcggat
     7921 agcgtacccg gatttgtatt ttgacggccg cgttttggcg gaaaaaaaga ttgataaaga
     7981 agaagcgtcc gattatatta gcgcggtttt tgaacaatcg ctgccgatca tctatagagc
     8041 gtattcgggc acgcggttaa agcgcggtct agtaccgtcc gatcctatat ccgaagctgt
     8101 acgtaagagc ttccattctc gcctgagcgg ggatgtcgtg atagtgctaa aaccttttca
     8161 cgtatgggat tccaacggcc taggaaccga acatagtctt ccgtatgcgt atgacgcgca
     8221 ggtgcctttg atattcaaag gaaaacgctg gatcaaaagt ggattgtaca aagacgaagt
     8281 cgcaccgacc gatatagttc ccgttttgtc ggtgatcacc ggttgcgaag atccgggcgg
     8341 tagagacgga caagtgcccg accagttatt gaaattttca aaatagttca tacatctaaa
     8401 caggagttca gttcaatgcc gggttttctc acaacaatca aatatgtgtg catatacgca
     8461 tcggattttg accgttcatt ggcattttat cgtgacggcc tcggcctcca ggtaacatac
     8521 aaagaagacg gttttgcaca gtttaatacc gaaggcacga tactcacgct cgaaaaagac
     8581 gggtttcgtt ccgatgagcc caaagactac cgcaaagtgg gtttcttaat tcaatttgaa
     8641 gtggaagata tcgaaaaagc ggttactgcg cttaaagcca aaaatgtgaa attcacccaa
     8701 acgattacgg ataaagaatt cggtcgtacg gcgatcatcg ttgatccgga cggcaaccag
     8761 atcatgctgc tggaacagta aacgggcaga tcggattcat tttttttggg tgcgaatgta
     8821 aaatttcaca gaggttctca tggctaatat tacatatctt gaagccattt cgatgggttt
     8881 gcgcgaagag atgcagcgcg acggtaatgt atttttgatc ggtgaagaca tcggtgtcta
     8941 tggcggcgcg ttcaaagtga ctaaaggatt tatcgacgaa tttggcgccg agcgtgttat
     9001 ggatacgccg atttccgaag ccggtattat cggcgtgtgt gtaggctccg cacttatggg
     9061 gatgcgtccc gtggcggaaa tgcagtttgc cgattttatt tcctgcggat tcaatcagct
     9121 tgtcaatcag gccgctaaaa ttcactaccg atggggcgag gctgtaccga tggtcgttcg
     9181 ttgtccctcg ggcggcggcg tgcacggcgg cccttttcac tcacaaaatc ccgaagcatg
     9241 gttttttcac gtaccgggat taaaagtggt ttccccgtca acgccctacg atgccaaagg
     9301 gctgatcaaa gcagcgattc gtgataataa tcccgtatta tattttgagc acaagttttt
     9361 gtatcgtcgt atcaaagggg aagtcccgga agacgactac atcgtgccga tcggcaaagg
     9421 ggacatcaag cgcgaaggta aagacatgac ggtcgtggcg tacagttcgg ccgtacattg
     9481 ggcgcttgaa gcggcagaga tcatcgaaaa agaaggcgtc agtgtcgaag tgctcgatct
     9541 gcgcagcatt ttaccgtacg ataaggaact gattctccaa tcggttcgca aaaccaatcg
     9601 agtcatcgtc gctcacgaag ctacgctaac cggcggggtc ggtggcgata ttgcagcgtt
     9661 catcaccgaa cacgctttcg aatcgctcga tgcgccggtg aaacgacttg cggcgattga
     9721 tacacccgtg ccgtacagcc cgccgctcga agcgtatttc cttcccaata aagacaaaat
     9781 gctggcgatg atgcgcgagc tcgcggctta ctgatcgaac atcatgcaga aacgatggtt
     9841 taatatattt tcaataattt cactggtact ctttggtgcc ggtgatttta ttttgaaggc
     9901 ctcggataaa ggcgagggac aggaacttca tgcaaaccgt tttcttgcgg atgctttgga
     9961 agacgcccgt cataattatg caggtcgcac tattcccgca tcggctgtcg tgaagtttga
    10021 agtgtatcgc ggttttcttg cggatatcaa taaagacggc gaatccgaat atatcgtggc
    10081 cggcacgatt acgggcctgg atatcaatta cgtcatggtg tatctgtgga agaacgaaaa
    10141 atggctctgt acagttctca atagcggtct cggtggaggc attgccgact tacaattgtt
    10201 g
//
LOCUS       DAOQNQ010000098        10158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2390_length_10158_cov_7.741562, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000098 DAOQNQ010000000
VERSION     DAOQNQ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10158
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2390_length_10158_cov_7.741562"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2321
                     /locus_tag="PK754_07670"
     CDS             <1..2321
                     /locus_tag="PK754_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09419.1"
                     /translation="AKEANNEKIRSLQLRNEEIRDSLKAWRLSASMDSSIYRDSLQND
                     SLLKVALNFKNMIKSESFSRGFSLNFSKAKNENDFWLLRWTINNITSNFNWSETETNT
                     PTSPQSTSRDWSSNTAYNISFARKSYKPLRWLPLTKTPFIGVVFKNISETDLNYKFIT
                     SANTDFRIDSKYRSETIRDAYNNPIKKPVNTTLSGSRGYGLSVSPWQSLTSSMNVKYN
                     MDLQGLSYGQILKGVGRGLNPFDGFNDFRFDSPLDTIRGIDDSLIFNRDYSSTNTFVL
                     SYSPQSFTWLTHTINYNAATGSSRNRAANSQLNKSATIQRSVQISSSFSLKSFIGSVK
                     EPFKNIDTRQPLSATEDSRKNRRDRFKNPGSDNSAKSSDKDSGNVITKGSSFFKTASL
                     RAGSTFEKILLMINDTRFNIDFKNSFNLGALENQLDPAAKWFGYMSTDNSGMLDHIFT
                     WDLEPINQYRTPVDSVLKQQVKSLTTNSKSINYGFMYGFNFGPFTLDIKYDRSETRSI
                     STNNTQDISIRRSSLFPYLKFVPFPFYEMTLRASNVGRWPIFNLLAGATRNINFSIGY
                     LSNENITLNEIRSRVDGIIFYNNDGFVDSSRTIQNIGGRRMQTVSMDKSITFPQISFD
                     ISWKGNVTQNISFSKSNTEKLNQNTTINQNSINILSNISYSKRGGFRIPIWFLKKKQL
                     DNEVRMSSQFTFRKTQEYSQSNTRSNDGFRQPKQKTSETTYWSVEPRIDYSFTRWVTG
                     GTFYRYEYNKSLTLGKTTRVLFGLNVNITIGA"
     gene            2582..4591
                     /locus_tag="PK754_07675"
     CDS             2582..4591
                     /locus_tag="PK754_07675"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB09420.1"
                     /translation="MTNKQNLLKIKIERKNKEIYTLLNQESETERLNAVRCLGEFQDS
                     NSILIIEEKIKTEKSDLVYNAYLFALGQLGFLNNHNLNLRIENFLIEQISPNKDTPYQ
                     LYVEALSKVGYQNSIIKILEIAKIDTNPTLKKHINLSLARLANRNQKNTDVFTYLKES
                     ITSKNDDVRWSAAYAYMRISDTNTVKYILDYVKDSDPRVRMDIVRSLGNMKISKTNEF
                     YKTTINTLIEILHNDPDWRVRSNAASGLGNYKFDFEEIKKIYYLTALEKTKDKNEHVR
                     ISAIRSIAKSFSGDIKPNNVFLKDFFDRYLTNTEKNMENAEIFNALCLMFGNKLLENG
                     DFIEYCKNVNQIKNAYVRAKIFESLSSLTSPDLTVYYKTALNDSFLLVRYQAIEGLGK
                     INNSEARDLIVQSINTSDPTLLSIVAGKLAEDTVYRKDDRKKEVLTKKIIEAFESIQK
                     PFDTEGKIGVIDALIQINHKSSYPFIRTFIKDPENKISTYVAEQLEKITGVSYKDSLP
                     KTNTNKSLEYKKLDQIFVKKPKAKIHTSKGLIELVFHASEAPISVLNFVELAEKKYFD
                     GVYFHRVVPNFVIQTGDPTGTGWGGPGYSIVSEYNTLHYSRGVVGMASAGKDTEGSQW
                     FITHSDQTHLDGRYSIFASVENGMEVVDQIQVGDQVKKIEIIWNE"
     gene            complement(4595..5743)
                     /gene="hemW"
                     /locus_tag="PK754_07680"
     CDS             complement(4595..5743)
                     /gene="hemW"
                     /locus_tag="PK754_07680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNB09421.1"
                     /translation="MKNKSFGIYVHIPFCERVCIYCDFFVTTSRKYRPAFIESINREI
                     HYVASQLEAPEARTIYLGGGTPSFLEDGQITSILNSIYGNFIKSENCEITIEVNPNNV
                     NSEKLNEFNRSGINRLSLGIQSLNDTELKSLYRTHNGEQAVSAFVTARKAGFKNISVD
                     VIFGLPGQTLENLQNTVTGIIEMDPEHISIYNLTIEEQTHLHKMVKAKKVVLLDDERE
                     FEMFSWLSEYLEKSGYSQYEISNFAKDGFESKHNSSYWNGLSYLGFGPSAHSFDNSRR
                     WWNVRDLSKYIAYAGIENQKIIDAEENLNINERITEFLFLNLRQRKGINKEIFLDAFG
                     ISFDAMFEKALSEAKPYINNTSDIVFLNQAGMFLYNQVCLLFVKNLNE"
     gene            5984..6640
                     /locus_tag="PK754_07685"
     CDS             5984..6640
                     /locus_tag="PK754_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09422.1"
                     /translation="MKRFLGQAMMMALIAMLAVSLTSIDAMAQKDKKKKKKKKDGPPE
                     WISKPGLYEDVIVAAGVGEGFNEQKAKSQAEQNGRKKIAEALSTEVKSLTTNFMEEAS
                     TTTEEGSSGAAQEYFSEVTQSMTNQTLSGAMIEEYWPPMGEKNGGKIKFYAKVVLKKS
                     AVVDAYKKQVEEDIAKKKIKSVKGSADDALKALDNAIGKWEKTNDNGPSTSEEGGAEE
                     "
     gene            6721..7314
                     /locus_tag="PK754_07690"
     CDS             6721..7314
                     /locus_tag="PK754_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09423.1"
                     /translation="MKSVQMFLISTLLVIMSCGGGKATSKNENKIPKWVSKPGLYEDV
                     IVAAGIGEGLSEQKAKSQAEQNGRKKIAEALQTQVKSLTTNFMEEAGTTTEKGTESAA
                     QEYFQEVTQTLTNITLNGAVLEEYWPPMGEKDGNRIKFYAKMVLKKSSLVDEFKKKVA
                     DDIAQKKIKNVKASADEALKALDKAIGKWEKTSESAD"
     gene            7488..9014
                     /locus_tag="PK754_07695"
     CDS             7488..9014
                     /locus_tag="PK754_07695"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HNB09424.1"
                     /translation="MEMQSKIKFLWLVIFLCFVQTNLWSQGPAPQWVNELPMKDGTLY
                     AVGLVNRYYEKKAGEEAADSAARSELSQTLKLNIKTTVQSWSGTIGGTGFIQELEKTI
                     DQDVFESVREAQVVGRWVDPEGLTYSLVSMPLSGVSTIQNKIVAFAKENSGGKKEAKK
                     IEKLEVSLKGLKDPKSYIRSSKPDWINGLPEEDDAIYALGIAEKFYFYVNGRESAKDK
                     ARAEIAATVKTEVNAILTDWYEVNEGSASYGSQQSFMEEMSNQVSEATLSGTQIVETW
                     YDKSTKWHYALARMSLSKVVGQIQDKAKSKVKDEKALKGLSDKLGKMINRDYLKSKDG
                     LPTWINKLPKDKEAIFAVGIDNGKYFSETQGIEKAKADARTKLAKTVSVTVENITKSW
                     IEIEEKSGDTKGEPLNDYMSSLTKEATDVSLQGSQVLSVYTKRDKDGKVTYYALGRLF
                     TGSMISKLKKKASEVMNIPQTKPNETQEQKAKRILSSGREKAAAALERLNAALDKVNQ
                     "
     gene            9045..>10158
                     /locus_tag="PK754_07700"
     CDS             9045..>10158
                     /locus_tag="PK754_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09425.1"
                     /translation="MKRCMWMLAMALIVAALLPMDQAQAQKKKSKDDKKSKSKKGGTP
                     EWVTKPGLYEDVIVAAGIGEAMSEQAAKSEAETDARKKIAQVLQTQVQSLTTNFMEEA
                     NTTTEAGSTGAAQEYFSEITQTMTDLTLKNVIIEEYYPPQGEKNGKKIKFYAKAVLSK
                     TDFADKFKNQVSTDVASGKINGIKMSANDALARLNKVLGGWGQDEEISSATDKPTATT
                     GLGAGISKPGGIGDTPTTTAQTGSKAASYPDWKKSLPNRPSRPGYYQGLGVAPITSNQ
                     AQDEKDAESEARAQVIRAIRSEITSKVTSAMEERSSSTGGSSSGSYSESFNSVTESFS
                     SESLKNLIVEFHTDKSSKKRYAYCEISIEEVNRQFAE"
BASE COUNT         3669 a         1773 c         1934 g         2782 t
ORIGIN      
        1 atgccaaaga agccaataac gaaaaaataa gatcattaca attacgaaac gaagaaataa
       61 gagatagtct taaagcatgg agattgtcgg cctcgatgga ttctagtata tatcgtgaca
      121 gcttacaaaa cgatagctta ttgaaagtag cattaaattt taaaaacatg ataaagtcgg
      181 aatctttttc acgaggtttt tctttaaatt tttctaaagc taaaaacgaa aatgactttt
      241 ggctcttgcg atggacaatc aacaatataa cctctaactt caactggagt gaaaccgaaa
      301 ccaatactcc aacatcacca cagagcactt ctagagactg gtcaagtaat acggcttaca
      361 atatttcttt cgcaagaaaa tcatataaac ctctccgttg gcttccatta actaaaacgc
      421 cttttattgg tgttgttttt aaaaatataa gcgagaccga tttaaattat aaattcatta
      481 cttctgcaaa caccgatttc cgaattgact caaaatatcg ttcagaaaca ataagagacg
      541 catacaataa cccaataaaa aaacctgtta ataccacatt aagcggctcg cgaggatatg
      601 gactgagtgt aagcccttgg cagtcattga cgtcctcaat gaatgtcaaa tataacatgg
      661 atttacaggg tctttcctat ggtcaaatac ttaaaggagt cgggcgcggg ttgaaccctt
      721 ttgacggttt taatgatttt cgctttgatt caccgcttga tacgattcgc ggcatagacg
      781 actctcttat ttttaatcga gactattcct caaccaatac atttgtgtta tcttatagtc
      841 ctcaaagttt tacatggctc acccatacta tcaattataa cgccgctacc ggaagcagtc
      901 gtaatagagc ggctaactca caactcaaca aaagcgcaac gatacaaaga tcggtccaga
      961 tttcttccag cttcagtttg aaatccttta tcggaagtgt taaagagccg ttcaaaaata
     1021 tcgatacaag acaaccatta tccgcaacag aggattcgcg aaaaaataga cgtgatcgtt
     1081 tcaaaaatcc aggaagtgat aattcggcta aatcatctga caaggactcc ggtaatgtaa
     1141 tcactaaagg ttcttcattt tttaaaacag catctcttcg tgccggatca acgtttgaga
     1201 aaatactttt gatgataaat gacacccgtt ttaatataga ttttaaaaat tcttttaacc
     1261 ttggcgcttt agaaaatcaa ttagaccctg cagcaaaatg gttcggatat atgagtaccg
     1321 ataatagtgg tatgttggat catattttta catgggattt agaacctatt aaccaatata
     1381 gaacacctgt ggattctgtt cttaaacaac aagtaaagag tttaactaca aactcgaaat
     1441 ctattaacta tggtttcatg tatggtttta attttgggcc atttacactc gacattaagt
     1501 atgatcgttc ggaaactcgg agtatttcaa caaataacac gcaagatatt agtatcagac
     1561 gctcttcttt gttcccatat cttaaatttg ttccttttcc tttttatgaa atgacgttac
     1621 gtgcatccaa tgttggtcgg tggcctatat ttaatttatt ggccggagca actcgtaata
     1681 taaactttag cattggctat ttatcaaacg aaaatataac tcttaatgaa atcaggtcgc
     1741 gtgtggatgg aattatcttt tataataatg acggctttgt tgactccagt aggactatac
     1801 aaaatatcgg cgggcgccga atgcagacag tatctatgga taaatctata acctttccac
     1861 agataagctt tgatatttcg tggaaaggta atgtgacaca aaacatatcc ttttcaaaat
     1921 caaatacaga aaaattgaat cagaatacga caatcaatca aaattctata aatattttat
     1981 ctaatatatc ttattctaaa cgaggtggtt ttcgtattcc aatttggttt ttaaagaaaa
     2041 aacaacttga taatgaagta cgaatgtctt cacaatttac atttagaaaa acccaagaat
     2101 attcgcaatc aaacacacgc tcgaatgatg gttttaggca gcccaaacaa aaaacatctg
     2161 agacgacata ttggtcggtt gaaccaagga ttgactattc atttacacgt tgggtaacag
     2221 gtggaacttt ttatagatat gaatacaaca aaagtttaac attagggaaa acaacacgcg
     2281 ttctatttgg attaaatgtt aatatcacga tcggcgctta aaaattgatg taaaaccatt
     2341 gttgatataa gaaatacttt atcaacaatt tatacctatt aacaagaaat taatacttac
     2401 taataatcca ataacatgtt tgaaaatata aacctttaaa atacagacca aaaaaaagtt
     2461 cttcacatat caacaaatct atactactac tactactata ataaataaat aagaataata
     2521 gaggtatata atgtataaac tctcatttct aattgcgtta ctgatttcct gtaacaatga
     2581 agtgaccaac aaacaaaact tgttaaagat taaaatagaa agaaaaaata aagaaatata
     2641 tacgcttttg aatcaagaat ctgaaacaga acgattaaat gcagtccggt gtttaggaga
     2701 atttcaggat agtaatagta ttcttattat cgaagaaaaa ataaagacgg aaaaatctga
     2761 tttagtatat aatgcttatt tatttgcatt gggacaatta ggttttctta ataatcataa
     2821 tttaaattta agaatagaaa actttcttat agaacaaatt agtcctaata aagatacacc
     2881 gtatcaattg tatgtggaag cgttgagtaa agtagggtat caaaattcaa taataaaaat
     2941 tttagaaatt gcaaaaattg atacaaaccc aactttaaag aaacatatta atttgagttt
     3001 agcaagactt gccaatcgaa atcaaaaaaa tacagacgta tttacgtatc ttaaagaaag
     3061 tattaccagt aaaaacgatg atgtacgatg gtcagccgcc tatgcatata tgcgtatatc
     3121 tgatacaaat acagtaaagt atattttaga ctatgtaaaa gatagtgatc cgcgagttcg
     3181 tatggatata gtacgatctc ttgggaatat gaagattagc aaaaccaacg aattctataa
     3241 aacaacgata aacacattaa ttgaaattct gcataatgac cctgattggc gtgtccggtc
     3301 caatgcagcc tcaggtcttg ggaattacaa atttgatttc gaagaaatta agaagattta
     3361 ttatttaacg gcattagaaa aaacaaaaga taaaaatgaa cacgtaagaa taagtgcaat
     3421 acgatcgata gcaaaaagtt tttccggtga tataaaacct aacaatgtat ttcttaagga
     3481 tttttttgat cgttatttaa cgaatacaga aaaaaacatg gaaaacgcag aaatttttaa
     3541 cgcgctttgc cttatgtttg gaaataagct tttagaaaac ggagacttta tcgaatattg
     3601 taaaaatgtg aatcaaataa aaaacgcata tgttagagct aaaatatttg aatcattatc
     3661 tagtctcaca tctccagatt tgacggttta ttataaaaca gcattaaatg attcattttt
     3721 acttgttcga tatcaagcga ttgagggctt gggtaaaata aataatagtg aagcaaggga
     3781 tttaatcgtt caatctatca atacgagcga ccctacatta ttgtcaatcg ttgcaggaaa
     3841 acttgccgaa gatacggtat accgaaaaga tgacaggaag aaagaggtgt taaccaaaaa
     3901 gatcatagag gcttttgaat caattcaaaa accttttgat acggaaggaa aaataggtgt
     3961 catagatgcg ctgatccaga ttaaccataa aagctcttat cctttcataa ggacttttat
     4021 caaagatcct gaaaacaaaa tttctacata cgttgccgaa caacttgaaa aaattaccgg
     4081 tgtatcgtat aaagattcat tgccaaaaac taatacaaat aaatctttag aatataaaaa
     4141 attagatcaa atttttgtaa aaaaaccaaa agcgaaaatt catacatcta aaggattaat
     4201 tgaactggtt tttcatgcaa gtgaagcacc gataagtgtt ttaaattttg ttgaattagc
     4261 tgaaaaaaaa tattttgacg gtgtttattt tcatcgtgtt gttcccaatt ttgtaattca
     4321 aaccggcgat ccgacgggaa ccggttgggg tggtccaggt tattcgatag tatcagagta
     4381 taacacgcta cattattcac gaggtgtagt cggaatggca agcgcgggta aagataccga
     4441 aggctcacaa tggtttataa ctcattcaga tcaaacgcat cttgatggga gatattctat
     4501 ttttgctagc gtagagaacg ggatggaagt tgtagatcaa atacaagtcg gcgatcaagt
     4561 aaaaaaaata gaaataatat ggaatgaata aaacttattc attcaaattc ttaacgaata
     4621 acaaacaaac ctgattatat aaaaacatac cagcttgatt taaaaaaaca atatctgacg
     4681 tgttatttat atacggtttt gcttcggaaa gggctttttc aaacatcgcg tcaaaagaaa
     4741 taccgaacgc atccaaaaaa atttctttat taataccctt tctttgcctc aagtttaaaa
     4801 aaagaaactc ggttattctt tcatttatat ttaaattctc ctcggcgtca attatttttt
     4861 ggttttctat tcctgcgtaa gcgatgtatt tagacaggtc gcgaacattc caccatcgtc
     4921 gcgagttatc gaagctgtgt gccgagggtc caaaacctaa gtatgataac ccattccaat
     4981 acgatgagtt atgttttgac tcaaacccgt cctttgcaaa attagatatt tcgtactgag
     5041 agtaacctga tttttcaagg tactctgaaa gccaagaaaa catttcaaat tctctttcat
     5101 cgtcaagcag cacaaccttt tttgccttta ccattttatg gagatgggtt tgttcttcta
     5161 tggtcaggtt gtaaatcgaa atatgttcgg gatccatttc tattatacca gtaaccgtat
     5221 tttgcagatt ttccagtgtt tgtcccggca agccaaaaat aacatcaaca ctaatgtttt
     5281 taaaaccggc ttttctagcc gttacaaaag cacttacagc ttgctcacca ttatgagttc
     5341 tatataaaga ctttaattct gtatcattca atgactgtat tcccaaactt aacctgttaa
     5401 ttcctgacct attaaattca tttaattttt cggaattgac attgttgggg ttgacttcta
     5461 tggtaatttc gcaattttca ctttttataa agttgccata aattgaattt aaaatggatg
     5521 taatttgacc atcctcaaga aaagaggggg taccgccgcc taaataaatt gtacgagctt
     5581 ccggagcttc taattgagag gcaacataat ggatttctct gttaatggat tcgataaaag
     5641 caggtcggta tttgcgtgac gtagtgacaa aaaaatcgca ataaatacac actctctcgc
     5701 aaaatggaat gtgcacatat attccgaaac ttttgttttt caaagcttta ttactcatta
     5761 aattgttatt tacctcatga aattagttta tatcaatagt gtttcaaaga ctaaaacgta
     5821 attttaaaaa acttgacttt atataagggc ttaggtaaat tttgaaacaa tcaagaatag
     5881 tctttccttg cgccgttctc aataaaatta aattttaaca aaccaatgtg ctctaatttt
     5941 gcataagttc ctctattcat ttatttaact caaggagtgc tgtatgaaac gttttttggg
     6001 tcaagccatg atgatggcgt tgatcgcgat gttagctgtt tcgctaacaa gcattgacgc
     6061 gatggcacaa aaagacaaaa agaagaagaa aaagaaaaaa gatggaccgc cggagtggat
     6121 ttccaaaccg ggtctgtatg aagatgtgat tgtcgcggca ggtgtaggcg aaggttttaa
     6181 tgaacaaaag gctaaaagcc aagctgaaca aaacggtcgt aaaaagatcg ccgaagcgct
     6241 ttcaacagaa gtaaaaagtc tgacaaccaa tttcatggaa gaagcatcaa caacaacaga
     6301 agagggttcc tcaggagcag cgcaggaata tttttctgaa gtgactcaat cgatgaccaa
     6361 tcagacgttg agtggcgcga tgattgaaga atattggccg ccaatgggcg agaaaaatgg
     6421 cggaaaaata aagttctacg ccaaggtagt gctcaaaaaa tcagccgtag tagacgcgta
     6481 taaaaaacaa gttgaagaag acatcgccaa aaagaaaatc aaaagtgtca aaggttctgc
     6541 cgatgacgct ctgaaagctc ttgataatgc gatcggcaaa tgggaaaaaa ccaacgacaa
     6601 cggtccttca accagcgaag aaggcggcgc agaagaataa aagtaatttt tagtaatgct
     6661 aaccgtagct tcgctttgat gaggctacgg ttattttttt gaaatctgat aggagtaggt
     6721 atgaaatcag ttcaaatgtt tttgattagt acacttttgg ttatcatgtc ttgtggtggc
     6781 ggtaaagcga catccaaaaa tgaaaataaa attcctaaat gggtcagcaa acccgggctt
     6841 tatgaggatg taattgtagc ggcaggaata ggagaagggc tttccgagca aaaagcaaaa
     6901 agccaagccg aacaaaacgg gcgcaaaaaa atagccgaag cacttcagac acaagtcaaa
     6961 agcctcacaa cgaacttcat ggaagaagcc ggaacaacca cagaaaaagg tactgaatcg
     7021 gctgcacagg aatattttca ggaggttact caaaccctta ccaacattac tctcaacggc
     7081 gctgttttgg aagaatactg gccaccgatg ggagaaaaag acggaaacag aatcaaattt
     7141 tacgcaaaaa tggtgttaaa aaaatcatcg ctcgtagatg aatttaagaa aaaggttgcc
     7201 gatgatattg cacagaagaa aatcaaaaat gtaaaagcca gtgccgacga agcgttaaaa
     7261 gcgcttgata aagcgatcgg taaatgggaa aaaacatcgg aatctgcaga ttagttaata
     7321 attattcggg ctgtagccat taaataaaat gatacagccc gtttttttaa attagtctta
     7381 tacaaatact gcgacataac ttgattttga gaagctattt ggaacacatt accacattta
     7441 aggttcttac atattcgatt tcgcttcaag tttgtatgag ggcaattatg gaaatgcaat
     7501 ctaaaatcaa atttctgtgg cttgttattt ttctttgctt tgtacaaact aatttatggt
     7561 cacaaggccc ggcaccgcaa tgggtcaatg aattgccaat gaaagacggt acgttgtacg
     7621 ccgtaggatt ggtcaatcgt tattatgaaa agaaagcggg agaagaagcc gccgatagcg
     7681 ccgccagatc ggagttatca caaacgttga aactaaatat taaaacgaca gttcaaagtt
     7741 ggtccggaac cattggtggt acaggtttca ttcaagaatt agaaaaaacg atagatcagg
     7801 atgttttcga atccgtgcgt gaggcacaag tggtgggtcg ttgggtggat ccggaaggat
     7861 tgacttactc tttagtatcc atgcctcttt cgggagtgag tacaattcaa aataaaatag
     7921 tagcgtttgc caaagaaaat tccgggggga aaaaagaagc aaaaaaaata gaaaaattag
     7981 aagtttctct caagggctta aaagatccca aatcatatat acgttcctca aaaccggatt
     8041 ggataaacgg gttacccgag gaagatgatg ctatatacgc acttggcata gctgaaaaat
     8101 tttattttta tgttaacggg cgtgaatcgg ccaaagataa agccagagca gaaatagcgg
     8161 caactgtaaa aacggaagta aatgctatac taacggattg gtatgaagtt aacgaaggta
     8221 gcgcttcgta cggatcccaa caaagtttta tggaagaaat gtccaatcaa gtgtctgaag
     8281 cgacactaag cggtacgcaa atcgtagaaa catggtatga caaatctacc aaatggcatt
     8341 atgcacttgc tcgcatgtcc ctaagcaaag ttgtcggcca aatacaagat aaagcaaaaa
     8401 gtaaagttaa agatgaaaaa gctctaaaag ggttaagcga taaactcggt aaaatgatca
     8461 atcgcgatta tctcaaatcc aaagatggtt tacccacgtg gatcaataaa ttgcctaagg
     8521 ataaagaagc aatatttgca gtgggtattg acaacggaaa atatttttct gaaacacaag
     8581 gtatagaaaa agcgaaagcc gacgcaagaa ccaaattggc taagaccgta tccgtgacag
     8641 tggaaaatat cacaaagtca tggattgaga tcgaagaaaa gtcgggcgat acaaaaggcg
     8701 aaccactcaa tgactatatg tcctccttga ctaaagaagc aacggatgtc agtttgcaag
     8761 gatctcaggt actatccgta tatacaaaac gtgataaaga tggaaaagta acatattatg
     8821 ccttaggaag gctatttacc ggaagcatga tttctaaatt aaagaaaaaa gcatccgaag
     8881 taatgaatat accacaaaca aaacccaacg agactcaaga acaaaaagca aaacgaatac
     8941 ttagttcggg tcgagaaaaa gccgccgctg ccctcgagcg attgaacgcc gcattagata
     9001 aagtaaatca ataatatata taaatactac cggaggatat gataatgaaa cgttgtatgt
     9061 ggatgcttgc gatggcgctt attgtggccg ccttactccc tatggatcaa gcacaagctc
     9121 agaagaaaaa atcaaaagat gataaaaaat cgaaatccaa aaaaggcggc acgccagaat
     9181 gggttaccaa accgggctta tatgaagacg ttatagtcgc tgccggcata ggcgaagcaa
     9241 tgtccgagca agccgcaaaa agtgaagcag aaacagatgc acgaaaaaaa atcgctcaag
     9301 tcttacaaac tcaggtgcaa agcctgacaa ccaattttat ggaggaggcc aatacaacga
     9361 ctgaggcggg atctaccggc gcggcacagg aatattttag tgaaataacc caaaccatga
     9421 cggaccttac gctcaagaat gtaatcatag aagaatatta tccgccacaa ggtgaaaaaa
     9481 acggaaaaaa aatcaagttc tatgcaaagg ctgtattgag caaaacagat tttgcagata
     9541 aatttaaaaa tcaggtttct acagatgtag catctggtaa aataaatggc attaaaatga
     9601 gcgccaacga cgcactagcc cgtttgaata aagtcttagg cggctggggt caggatgaag
     9661 aaatcagcag tgctacagat aaaccaaccg cgacgacagg tttgggggct ggaataagca
     9721 aacccggcgg aatcggagat acacctacga cgacggcaca aaccggaagt aaagccgcca
     9781 gttatccaga ttggaaaaaa agcttaccca atcgtccatc gcgtccagga tattatcaag
     9841 gtttgggtgt agcgccaatt acttcaaatc aagcacaaga tgaaaaagac gctgaatcgg
     9901 aagcgcgagc tcaagtcatt cgagcaatcc gttctgaaat caccagtaaa gtaaccagcg
     9961 ccatggaaga gcgcagttca tctaccggag ggagttcctc gggaagctat agcgaaagct
    10021 ttaattccgt aacggaatct ttttcaagcg aatcacttaa aaacctgatc gttgaatttc
    10081 atacggataa atcatccaaa aagcgttacg cttattgtga gatatccatt gaagaagtca
    10141 atcgccagtt tgctgaac
//
LOCUS       DAOQNQ010000099         9972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2468_length_9972_cov_6.220329, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000099 DAOQNQ010000000
VERSION     DAOQNQ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9972
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2468_length_9972_cov_6.220329"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /locus_tag="PK754_07705"
     CDS             complement(<1..1467)
                     /locus_tag="PK754_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNB09426.1"
                     /translation="MQRLKGNSLFSGLPETEIAKLEEFIRIKDFPKNAVLIDEGVPGS
                     YLYLLIAGNVKITRNGLDGREMILTERHAGDFFGEMALLDDAPRSARVVATEDSVAGV
                     IGKNEFNAMMIREPRVAVNILRTISMRLRLSNDQIMQSMEEKERYHAKQLERFQSLIE
                     FNKTLGAEREEVGLFRVVPVIVRSQILCHDVLLVLDDPESDKYHTSIQEVSGWRMIDF
                     SETDKGTAKLAALKKTLTMEDFAAYEFRDTMEPQAFWKNAASIMIAPINGPRQNHGFI
                     AVKSAQKFRWDAEDEAFMTTLASDVSMVLRNMRLMRKAMFDEKMSAVGKASSSLLHDF
                     KNLLSVVYNYAKLIKTAATEEERKELVEKIVKFSSLMMGMSQEVLIYGDGSVKASPTP
                     VRISEIFDMVMPLLDTAGKKITMQTSIPEGMEFPLDKDKICRVFYNILTNACDAMGDA
                     GHISVTAAFEKNDLVIRISDTGPGIPEDVLPQIFKPFVT"
     gene            complement(1470..2972)
                     /gene="dacB"
                     /locus_tag="PK754_07710"
     CDS             complement(1470..2972)
                     /gene="dacB"
                     /locus_tag="PK754_07710"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNB09427.1"
                     /translation="MRLMFLWCILGIVWSPDLKSQSKKQNGSPAIKQLQADVLDDIGQ
                     KFVENASWGIVVQSLKNGETLVSVNPKKNLIPASNRKIFSSIFALDQLGADFTYVTQV
                     FAKGVLDSDSVFKGDIIIRGAGDPTISGRFYENKITKILEDWADSVQRKGIKSIRGRI
                     IGDDNGLMGDDVLGDYWESTEESYWYSAQISGLNFNDNCVDWYAKPTRIGEPAKIILI
                     PPTQYIKINNFVKTVSSKSEAVKVDAIRRRAANEVDFIGSILTESDPQHGYVTVENPT
                     RYTAHVFSEILQSRNIRVDSAAVDADDLVRYVYNETDSNMIRIARYVSPAMPELMKII
                     NKNSHNLYAELLWRTVAAVKDSSGTSKRALALEEKFLKSVGLNPDKISICDGSGYARG
                     NLVSPQDIVTLLTWVRKHKNWKTFYESLPLAGEEGTVKSRMKGTLAEGNARAKTGYLD
                     NVRTISGFVKTRDGEELVYSVMCNNFTADRLEIDKIQDMIIGRLAFFTRN"
     gene            complement(2988..4265)
                     /locus_tag="PK754_07715"
     CDS             complement(2988..4265)
                     /locus_tag="PK754_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNB09428.1"
                     /translation="MKSFHRRSYLTVIATLCFALPTLGQDIYTTGTPFNLYFTPFAIH
                     VGDFNRDGFMDVVTSGKNDLDGSGIISVLYGKGDGTFKNKIDLASEKFAVDIQVGDLD
                     NDGMPDIVTANGPAQTVSVFMNQKSGGFKAKDPIKIKGEPSLINLGDYDKDGKIDIAI
                     VIKATKELHIYKGTSYKLSAVKILDDVPSAIRSDDYSGNGFSHMVISYEAASYVTLAN
                     MVELKGYKYEINTAKIDMLALPQFTHHADGNNDGFEDAFLFDRNKSTIAVIKAEANGL
                     ILDNIFTLKVPAGSTSFCVGDFNKDKKADIALLDSANAQVTIYMNQQPDPAPQNTFAQ
                     GKLIIVYAPDFTKPNNADVLMSSAYKKVSMYLYYPDGKPARKYFDIESDLSDGQFTIE
                     WAGTDENDAELPNGEYYFYYQLGSLIITRGLKK"
     gene            complement(4442..5704)
                     /gene="hisS"
                     /locus_tag="PK754_07720"
     CDS             complement(4442..5704)
                     /gene="hisS"
                     /locus_tag="PK754_07720"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNB09429.1"
                     /translation="MTYRMPTGTKDVLPSDSYKWQYVERKIADVMRRFNYKEIRTPMF
                     EYTELFARGIGEATDVVGKEMYTFLDKGQESLTLRPELTASVMRSYIENSLGEQLPLN
                     KCWYMGPMFRQERPQKGRLRQFHQFGVEALGSASPEADVETMLVVLTVYEELGIKALN
                     FKINSVGCPQCRPAYRDALVAFLDGVKGQLSEESRKRLVVNPMRILDSKDERDIALTQ
                     SAPLMKDHLCTDCATHFSRVQSSLNGLGIVYEIDGRLVRGLDYYTKTAYEIQSGNLGS
                     QNALGGGGRYDLLAEEFGGKPTPAVGFAAGIERLMIVLEAEGIPIGEPEKLDVYFIAL
                     GAAAVGATMPLIQKARREGLTCDTDLLARSMKAQMRDANRMQARFTVILGDNEIAQNM
                     AVVKNMSDGAQEQISLNELTSYLKTKLA"
     gene            complement(5723..7450)
                     /locus_tag="PK754_07725"
     CDS             complement(5723..7450)
                     /locus_tag="PK754_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB09430.1"
                     /translation="MLNETSALFNSRSDNRVMLWSPFLATLCVTLVMAIWNVITYQRL
                     FDLSPDESFFVSVTGFLVVGLTIVVGGMMHLALFRKTLTAESHSGTLSEESIRRIWVE
                     WLKYPRTMVFYLYFVHLAGMSLLLSSVLLVPFVIESISGWEYMYKTLIWVLLGLIPFS
                     IILFYVLEWRIHVCSSRFPEVLAQMPDANDPDIVWIRLRTRVMAPILAMTLWYGLLLY
                     NYHNHPVAITLLALVMFGFIGWLTTRTVQTAHDRWLTLWSGPDIYHTEEVGHTMLHAG
                     DIYRLQKTAERFAATAKREVLVLRDQIQPLGATAEHILSGLKSQNSYASNHASALAQI
                     TASLEELMKSVAQISENSKSVNQSTLKGSQEAEDGKRHVADTLQSMEQIHRESQTSAQ
                     RILDLSLKMTQIEDVIKIINYITDQTKILAFNAAIETAGAGEMGERFGVVAKEIRHLA
                     QNIADSTEEIKKILLDVRKASQVSVLASEKELMQVKEGIRHAGDARESFEQIYWMVRQ
                     TMESVQQIAQAIQQQQSALEQLWMSIKDIDAGAKESLRQNKNMLSSAEELRMLSQNTR
                     TSQRPQSTA"
     gene            complement(7452..>9972)
                     /locus_tag="PK754_07730"
     CDS             complement(7452..>9972)
                     /locus_tag="PK754_07730"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNB09431.1"
                     /translation="LQKEASADTSDKKITETTEPVAEPVTPKKSSKTVTLDRGLFIKR
                     FLTEAEEHVRNLKQHTAAWQKQHDAASLEIAIRAAHTLKGSARMMKFENVGNISQRAE
                     FFFRGLKEKKLTWSAGADQVLVMAYQSLDDLIEKIRITEKDEFQNAALMDMLDEINAG
                     AVPTEEAVRDLIRKPAHPVGETRPVREVKSGASGPDRLGERLIEAGLITREELMHANQ
                     TTDTRMPLGERLVALGYINRDQLNQLLMEQKSSRELLGQTQINAGRITQKEEVLNSDQ
                     FVHVRIDKLDELIKSVGDISTKQIKTEEHIAVLRKIYLALKYWNSELRDYENAAQAGN
                     VIDNPHLSQRLSETADELADVIKIAREEAANFDLSLNAIQTGIMGMRMVPIRSIFDGF
                     PRAVRDLAKNLGKDVKLVVEGAETELDRKIVESLNEPLIHLIRNAIDHGLEPSPDRIA
                     AGKTAEGLLRIAAYNEGNTILIEVEDDGRGIDLERIKAKAIEKKIVTDEQDISRFSEN
                     DLINLIFLPAFSTSELITDISGRGYGMDVVKRAIESIKGYIEVVTAPNVGTRFSIHLP
                     LTLTSLRALFVKSAAYKFAVPMQAVAETLKIKHDDIIEVVEKKAIRLRNQLIPIVRLN
                     EVVGYAASDEERDERFLIIIHANGEKAGFVVDDIIDEREILVKSLPKQFAKVKHISSA
                     TVASNNEIIMVLHAPDLIRATKDFSVREQVQSIPKSARSILVVDDSLNTREIEKTILQ
                     AYGYDVETAKDGLDALEKIQQKKYDLVVTDLEMPAMDGLTLTSRIKSEASYQHIPVVI
                     VTSRDSAEDKRRGIEAGANAYIVKGSFDQTNLIDTVESLIG"
BASE COUNT         2483 a         2504 c         2196 g         2789 t
ORIGIN      
        1 ggtaacgaac ggtttgaata tttgaggcaa tacatcttcc ggtatacccg gtcctgtgtc
       61 ggaaatacga atcaccaaat catttttttc aaaagcggcc gtaacggata tatgccccgc
      121 atcgcccatc gcatcgcagg cattggtaag tatattataa aaaacacggc agattttatc
      181 tttatcgaga ggaaattcca taccttcagg gatggatgtc tgcatagtga tttttttacc
      241 ggcggtatca agcaacggca tcaccatatc aaaaatttcg ctgatgcgta ccggtgtggg
      301 actggctttg acggaaccat ccccgtaaat caacacctcc tgcgacattc ccatcataag
      361 cgacgaaaat ttcacgattt tttctactaa ttcttttcgc tcttcttccg tcgcggctgt
      421 cttgatcagt ttcgcataat tataaacaac cgacagcagg tttttaaaat catgcaacaa
      481 cgaactcgac gctttaccga cggcggacat tttttcatca aacatcgctt tgcgcatcag
      541 gcgcatattg cgtaacacca tagatacatc cgacgccagc gtagtcataa aggcttcatc
      601 ttctgcatcc catcgaaact tttgcgcgga cttcacggca ataaaaccgt gattttgacg
      661 cgggccattg atcggtgcga tcattatact ggccgcattt ttccaaaacg cttgcggctc
      721 catcgtgtca cgaaattcgt atgcggcaaa gtcttccatc gtcagtgttt ttttaagagc
      781 cgctaattta gccgtgcctt tatccgtttc actaaaatcg atcatacgcc agcccgacac
      841 ttcttgaatg cttgtatgat atttatccga ctcgggatca tccaatacta agagtacgtc
      901 atgacataat atctgactgc gaacaataac aggcacaaca cgaaacaatc cgacttcttc
      961 acgttcggcg ccgagtgttt tattaaattc gatcaaactt tggaagcgct ccaattgttt
     1021 ggcatgatag cgctccttct cttccatgga ttgcataatc tgatcgttgg acaagcgcag
     1081 acgcatgctg atcgttcgta gaatattgac cgctacccgc ggttcacgga tcatcatcgc
     1141 attaaattcg tttttaccga tcacaccggc caccgaatcc tccgttgcaa caacccgagc
     1201 cgatcgcggt gcatcatcga gtaacgccat ttcaccgaaa aaatcgccgg catgccgctc
     1261 ggtaagtatc atctcgcgac cgtccaatcc gttgcgcgtg attttaacat tgcccgcgat
     1321 caacagatac aaataacttc ccggcacacc ctcgtcaatc agtaccgcgt ttttcggaaa
     1381 atctttaata cgaataaatt cttcgagttt ggcaatttcc gtctcgggca aacccgaaaa
     1441 aagactgttt cctttcagtc gctgcatatt taattccttg taaaaaaggc aaggcgtccg
     1501 atgatcatat cttgtatttt atctatttcg agacgatctg cggtaaaatt attacacatg
     1561 acactataca ccaattcttc tccgtcgcgc gtttttacaa aacctgaaat agttcggaca
     1621 ttatcaagat atcctgtttt ggcacgcgca ttaccttcgg ccaaggttcc tttcatgcgc
     1681 gatttgacgg tgccttcttc accggccaaa ggcagtgatt cgtaaaaagt tttccaattt
     1741 ttgtgtttgc gaacccacgt caataatgtg acaatatcct gaggtgacac aagattgccg
     1801 cgcgcataac ccgatccgtc gcaaatactt attttatccg gattcagtcc gacggatttt
     1861 aaaaattttt cttccaacgc caacgcgcgt ttggaagttc ccgacgaatc tttcaccgcc
     1921 gccaccgtac gccataataa ctcggcgtat agattgtgac tgtttttgtt gattattttc
     1981 atcaactcgg gcatggccgg tgaaacatac cgtgcaatac gtatcatatt ggaatcggtt
     2041 tcattgtata catacctcac caaatcatcg gcatcaaccg cagcgctatc cacgcgtatg
     2101 tttctggatt ggaggatttc actaaatacg tgtgccgtgt agcgtgtcgg attttctacg
     2161 gtcacatacc cgtgttgcgg atcgctctca gtgagaatac tacctataaa atccacttca
     2221 ttggcagcgc gtcgccgtat cgcatctact ttgaccgctt ccgattttga cgagacggtt
     2281 ttaacaaagt tattaatttt gatatactgc gtcggcggta tcagaataat cttagccggt
     2341 tcgccgatac gcgtgggttt ggcataccaa tccacgcagt tatcgttaaa attgagtccg
     2401 ctgatttgtg ccgagtacca atacgattct tccgtagatt cccaataatc cccgagcaca
     2461 tcgtcgccca taagaccgtt atcatcgccg atgatgcgac cgcgaatgga tttgattcct
     2521 tttcgttgta cgctatccgc ccaatcttct aaaatttttg tgatcttatt ttcataaaac
     2581 cgtccggaaa tcgtcgggtc gcccgcaccg cgaattataa tatcgccctt gaaaaccgaa
     2641 tccgaatcca gtacaccttt agcaaaaacc tgtgtgacat aggtaaaatc ggcgcccaat
     2701 tgatccaacg caaaaatgga ggaaaaaatt tttcgattgg atgccgggat gagatttttt
     2761 ttggggttta cggaaaccaa cgtctcgccg tttttgagcg attggaccac aattccccac
     2821 gacgcattct cgacaaattt ttgtccgatg tcatcaagga cgtccgcttg caactgtttg
     2881 atagcgggcg aaccattctg ttttttggat tgtgatttga gatccggact ccacacgatg
     2941 ccgaggatgc accataaaaa catcaatcgc atgctgttca tttctcttta ttttttgagg
     3001 ccgcgtgtta tgatgaggct gcccagctga tagtagaagt agtattcccc gttaggtaat
     3061 tctgcatcgt tttcatccgt accggcccac tcgatcgtaa attgaccgtc ggataaatca
     3121 gattcgatat caaaatattt acgtgccggc tttccgtccg ggtaatagag atacatactg
     3181 acttttttgt acgcagacga catgaggacg tcggcattat tgggcttggt aaaatccggt
     3241 gcatacacga tgatcagttt tccttgcgca aaagtatttt gcggcgccgg atcaggttgc
     3301 tgattcatat aaatcgttac ttgcgcattg gccgaatcca aaagcgcaat atcggctttt
     3361 ttatctttgt taaaatcccc gacacaaaac gacgtcgaac cagcaggaac tttgagtgta
     3421 aatatgttat ccaaaataag accattggct tctgctttga tcaccgcgat cgtacttttg
     3481 ttgcgatcaa aaagaaacgc atcttcgaat ccgtcgttat taccgtccgc atggtgggta
     3541 aattgtggta aggccaacat atctattttg gccgtgttga tttcgtattt gtaacctttg
     3601 agttcgacca tattcgccag tgtgacatac gatgcggctt cataactaat caccatatgg
     3661 gaaaatccgt tgcccgaata atcatcgctg cgtatggccg acggtacgtc atcaagaatt
     3721 tttacggccg atagtttgta cgatgtgcct ttataaatat gcagttcttt tgtcgctttg
     3781 attacaatcg cgatatcaat cttaccatcc ttgtcataat cgccaagatt gatcagcgaa
     3841 ggttcccctt tgattttgat cgggtcttta gctttaaatc cgccggattt ttggttcata
     3901 aaaacagata ccgtttgcgc agggccgttg gccgtaacga tatcaggcat accgtcatta
     3961 tccaaatcgc ccacctgtat gtctacggca aacttctcgc tggcaagatc aattttattt
     4021 ttgaaagttc cgtcgccttt gccgtacaaa acggaaataa tacccgaacc gtctaggtcg
     4081 ttttttccgg aggtcaccac gtccataaat ccgtcgcgat taaaatcccc gacatggatg
     4141 gcaaatggcg taaaatagag attaaagggt gtgcccgtcg tataaatatc ctgtcccaat
     4201 gtgggaagcg cgaaacacaa tgtcgcaatg acggtaagat atgaacggcg atgaaacgat
     4261 ttcataaaaa atctaaacat actaaatgat attgccctac tcagaaagga agaaccaaat
     4321 taaatgggaa gaattttcct catgaagtga atatgtccaa taagagctat taaatcaata
     4381 gttattaggt agtttgcgtt ttgtagtata tccgattttg ccggctcttt ttcggattta
     4441 gttatgctaa tttagtcttt aagtacgacg taagttcgtt gagtgaaatt tgttcctgtg
     4501 caccatcgct catatttttt acgacggcca tgttttgagc gatttcatta tcgccgagta
     4561 ttacggtaaa tcgtgcctgc atacgattgg catcgcgcat ttgcgctttc atgcttcgcg
     4621 ccaataaatc cgtgtcacag gtcaaccctt cgcgtcttgc tttttgtatc agcggcatag
     4681 tggcccctac cgcggccgcg ccaagagcga taaaatacac atccaatttt tcaggttcgc
     4741 cgatcggaat accctccgct tctaaaacga tcatcaaacg ctcgatacct gctgcaaaac
     4801 ctacagccgg tgtgggttta ccgccaaatt cttccgccaa aagatcgtac cgtccgccgc
     4861 cgcccaacgc gttctgcgaa cccagattac cgctttgaat ttcatatgct gttttggtgt
     4921 aataatcaag accacgtacg agccgtccgt ctatttcgta cacgatgccc aacccattca
     4981 gcgaactttg gacgcgcgaa aaatgcgttg cacagtccgt acataaatga tctttcatca
     5041 acggcgccga ttgcgtcagc gcgatgtcgc gttcgtcttt ggaatccaaa atacgcatcg
     5101 ggttcacaac caagcgcttg cggctctctt ccgacaattg ccctttgaca ccgtcaagaa
     5161 atgccaccaa ggcatcgcgg taagcgggac ggcattgagg acaaccgaca ctattgattt
     5221 taaaattgag cgctttgata cccagctctt catatactgt aagtacgacc aacatcgttt
     5281 cgacgtcggc ttccggtgaa gcgctgccga gcgcttcgac tccgaactga tgaaactgac
     5341 gcagacgccc tttttgagga cgctcttgac gaaacatcgg gcccatatac cagcatttat
     5401 tcaaaggaag ttgctcgccg agactgtttt cgatgtatga gcgcataacc gaagccgtca
     5461 attcgggtcg caacgtcaga ctctcctggc ctttatctaa aaaagtgtac atttcttttc
     5521 cgacaacatc cgtcgcttcg ccgatcccgc gcgcaaaaag ttcggtatac tcaaacatcg
     5581 gcgtgcgaat ttctttgtag ttgaaacggc gcatcacatc ggcgatttta cgctcgacat
     5641 attgccattt ataactgtca gacggtaaca catctttggt tccggtcggc atacgatacg
     5701 tcacaaggta ctcctaaaag acttaagcgg tggattgcgg ccgctgcgat gtacgcgtgt
     5761 tttgagacaa catacgtaat tcttcggcgg acgataacat gtttttattt tgtcgtaatg
     5821 attctttggc gccggcatca atatctttga tgctcatcca gagctgctcc aaggcgctct
     5881 gctgctgctg gatcgcttgc gcgatctgct ggacggactc catcgtctgg cgcaccatcc
     5941 agtatatttg ttcaaacgat tcgcgcgcat ccccggcgtg ccggattcct tctttgacct
     6001 gcatcagctc tttttcgctg gccagtaccg atacttgcga cgctttacgc acgtcgagca
     6061 ggattttttt gatctcttcg gtggagtcgg cgatattttg cgcgagatgc cggatctctt
     6121 tcgctacgac gccaaatcgt tcgcccattt ctccggcgcc tgcggtttcg atagccgcgt
     6181 tgaaagccaa tattttggtt tgatccgtga tgtaattaat aattttgatc acatcttcga
     6241 tttgcgtcat tttgagactc aaatctaaaa tacgttgtgc gctggtttgc gattcgcgat
     6301 gtatctgctc catactctgc aatgtatccg ctacatgacg tttcccgtct tccgcttcct
     6361 gactgccctt gagcgtggat tgattgacgg atttggaatt ttcggatatc tgcgcaaccg
     6421 atttcatcag ctcttcgagc gacgccgtga tctgcgccaa cgccgaggca tgattggaag
     6481 cgtagctgtt ttgcgatttt aagccggaca aaatgtgttc agcggtcgca ccaaggggtt
     6541 ggatttgatc acgtaaaacg agcacctccc gtttggccgt ggccgcaaat cgttccgccg
     6601 ttttttgtaa tcggtatata tcacccgcat gcaacatggt atgaccaacc tcttcagtgt
     6661 gatatatgtc cggaccggac cagagtgtca accaccgatc atgcgccgtc tgcaccgtac
     6721 gggtcgtaag ccatccgata aagccaaaca tgacaagcgc caaaagcgta atcgcaaccg
     6781 gatgattgtg ataattatac agcaaaagac cgtaccacag cgtcatcgcg aggatcgggg
     6841 ccatgacgcg cgtacgcaga cgaatccaga cgatatccgg atcgttggcg tcaggcattt
     6901 gcgccaaaac ttcgggaaac cgcgaactgc atacatgaat tcgccactcc agcacataaa
     6961 ataaaataat ggaaaaggga atcagaccaa gcaataccca aatcaacgtt ttatacatgt
     7021 attcccatcc ggaaatcgac tcgattacaa acggcacgag caaaaccgat gaaagcaata
     7081 aactcattcc cgcgaggtgc acaaagtaga gataaaaaac catcgttcgg ggatatttca
     7141 gccattcgac ccatatcctg cgaatagatt cttcggatag cgtaccgcta tggctttcgg
     7201 cggtcaatgt ttttcgaaaa agcgccagat gcatcatgcc gccgactacg atcgtaagcc
     7261 cgacaacaag aaaccccgtc acagaaacga aaaacgattc gtccggtgaa agatcaaaca
     7321 accgctgata tgtgatcaca ttccaaatcg ccatcaccag tgtcacgcac agcgtggcta
     7381 aaaaaggtga ccacagcatg acccggttat ccgatctgga attgaataaa gcgctcgttt
     7441 cgtttaacat attatccgat caggctttcg actgtgtcaa taagattggt ttggtcaaat
     7501 gatcctttga cgatataagc attggcaccc gcttcgatac cgcgacgctt atcctcggcg
     7561 gaatcgcgcg atgtcacaat caccacgggt atgtgctgat acgaagcttc cgatttgatg
     7621 cgcgaagtca gcgtgagacc gtccatcgcc ggcatttcca aatcggttac cacaaggtcg
     7681 tatttctttt gctgtatttt ttccaaagca tccaacccgt ctttggccgt ttccacatcg
     7741 taaccatagg cctgcaatat ggttttttcg atttcgcgcg tgttgagcga atcgtccacg
     7801 accaaaatac tgcgcgctga tttgggaata ctttgcactt gttcgcggac tgaaaaatct
     7861 ttggtcgcac ggatgagatc gggcgcgtgc aagaccatga tgatttcgtt attggaagca
     7921 acggtcgccg acgatatatg tttgaccttg gcaaattgtt tcggcaatga ctttacgaga
     7981 atctcccgtt catcaatgat atcatccacc acaaatccgg ctttttcgcc attggcatga
     8041 ataataatca gaaaacgttc gtcgcgctct tcatcggaag cggcataacc gacgacttca
     8101 ttcaaacgca cgatgggaat gagctgattg cggaggcgaa tcgctttttt ctccacgact
     8161 tcgatgatat cgtcgtgttt gattttgagt gtctcggcca ccgcctgcat cggcacggca
     8221 aatttataag ccgccgattt tacaaacaac gctctcaggg aggtcagcgt caacggcaga
     8281 tggatcgaaa atcgcgtgcc tacattgggc gccgtgacca cttcgatata acctttgatg
     8341 gattcgatcg cgcgtttgac gacatccatt ccataaccgc gcccggatat gtcggtgatc
     8401 agttccgatg tactgaacgc cggaagaaaa atcagattga tcagatcgtt ttccgaaaaa
     8461 cgcgagatat cctgttcgtc ggttacgatt ttcttttcga tggctttggc tttgatgcgt
     8521 tcgagatcaa tgccccgccc gtcatcttcg acttcgatca gaatggtatt accttcattg
     8581 tatgcggcga tacgcagaag accttcggcc gttttgcctg cggcgatgcg atccggcgac
     8641 ggctctagtc cgtgatcgat cgcgttgcgt atcaggtgta tcaaaggctc gttgaggctt
     8701 tctacgattt tacgatcaag ttcggtttcc gcaccttcca caacgagctt cacatctttg
     8761 cctaagtttt tggcaagatc gcgtacggca cgcggaaatc cgtcgaatat gctgcggatc
     8821 ggcaccatgc gcattcccat gataccggtt tgtatggcat tcaaagacaa atcaaaattg
     8881 gccgcttctt cgcgtgcgat tttgatcaca tcggccaact catccgccgt ctccgataaa
     8941 cgttgggaca ggtgcggatt atcaatgaca ttgccggctt gggcagcgtt ttcgtaatcg
     9001 cggagttctg aattccaata tttgagtgcc agataaattt tgcgcagtac ggcaatatgc
     9061 tcttcggttt tgatctgttt ggtcgaaata tcaccgacgg acttgatcaa ttcgtcgagc
     9121 ttatcaatac gcacgtgaac aaactgatct gaattgagca cttcttcttt ttgcgtaata
     9181 cggccggcgt tgatttgcgt ttgacccaac agttcacgcg aagatttttg ttccatcaaa
     9241 agctgattga gctgatcgcg attgatataa cccaaagcga ccaatctttc gcccagaggc
     9301 atgcgggtat cggtggtctg attggcgtgc atcagttctt cgcgcgtaat caatcccgct
     9361 tcgatcaggc gttcacccaa acgatccggt cccgacgccc cgcttttgac ttcgcgtacg
     9421 ggccgcgttt cacccacggg atgcgccggt ttgcgaatca aatcccggac agcttcttcg
     9481 gtaggcacgg cacccgcatt gatttcatcc agcatgtcca tcaaggcggc attttgaaat
     9541 tcatcctttt cggtaatgcg gattttttcg atcagatcat ctaaactttg atacgccatt
     9601 accaacacct ggtcggcgcc agcactccat gtcaattttt tttctttcaa tccgcgaaag
     9661 aagaattcgg cacgctgact gatattgccc acgttttcaa acttcatcat gcgggcggaa
     9721 cctttgagcg tgtgagcggc gcggatagct atctccagcg acgcggcatc gtgctgtttt
     9781 tgccaggcgg cggtatgttg tttcaaatta cgaacatgtt cttcggcttc ggtgagaaac
     9841 cgtttgataa aaagcccgcg atccagcgtc acggttttgc ttgatttttt gggcgtgacc
     9901 ggctcggcga caggctctgt cgtctcggtg atttttttat ccgatgtgtc ggcgcttgct
     9961 tctttttgca gt
//
LOCUS       DAOQNQ010000100         9923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2481_length_9923_cov_6.553709, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000100 DAOQNQ010000000
VERSION     DAOQNQ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9923
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2481_length_9923_cov_6.553709"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PK754_07735"
     CDS             <1..276
                     /locus_tag="PK754_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB09432.1"
                     /translation="RKKALDKIGGFDTGLEFWSEDTQIGRRMSQEGRVRFFHRAYVYT
                     SARRYYAEGFLRVLMRYIMNYFWDIIFHRPFTKGYQDVRSTPPKQTT"
     gene            complement(290..1480)
                     /locus_tag="PK754_07740"
     CDS             complement(290..1480)
                     /locus_tag="PK754_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNB09433.1"
                     /translation="MIKKLFLFVSLCLTTAIWGQQIQYLHDEFSALPGDSLAEMEVWN
                     QVNIERTKAKLPPLVWDKALRSAARQHSREMMALGYFSHTSPTASLKTVSDRVYRTGA
                     SDASLGENIALHSVDGGYRSIADQLMKQWMNSPGHRANILKAEFTHIGIGIASYKDSV
                     IKDTLIRGAKTRLARYTIRHYGTQVFCERDLMMDSLFIEKRSDDFLVLDLQFQSDRSM
                     LVAAGPRVQFVNAIGNSVPIHIEIPYEPGMILTLARASNEQTKEYALFFQDTVKSVLS
                     LTQALSPSPVLLLRKDIAITRKERWFLRGTARLMHPTLATACHVYINKDGYYIASKRG
                     DRIEWSIPIEADADTALYAFAVGKEIQKIITNQIYINTAVLDYPDTNPAEKARSVFVK
                     QITP"
     gene            complement(1487..2101)
                     /gene="ruvA"
                     /locus_tag="PK754_07745"
     CDS             complement(1487..2101)
                     /gene="ruvA"
                     /locus_tag="PK754_07745"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNB09434.1"
                     /translation="MITFVQGLVAEKEPTRAVIDVQGIGYELFIPVSTYEKIPAVGAA
                     CKLLTYMHVREDIMQLFGFVSAEERSFFLLLISVSGIGPKSGISMMSGIAIRDFKAAV
                     LNEDVQLLTAIPGIGKKTAQRIVVELKDKITKTGDVASTAKLSTSSANLQVADEALMA
                     LISLGYSKPVAEKAIARLLREAGDKPFALQDMIKQALRLASTGA"
     gene            complement(2104..2820)
                     /gene="radC"
                     /locus_tag="PK754_07750"
     CDS             complement(2104..2820)
                     /gene="radC"
                     /locus_tag="PK754_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNB09435.1"
                     /translation="MGKPKKIAEPSLYHAKIKDWSSQDRPREKLEQLGAARLSSAELI
                     AILIGSGTRNITAVDLARALEKEFSGLAALARCSTHELRRFKGIGKVKSIKLAAAFEL
                     SRRIEAQSDAKKIRVRAPEDLFKHYMPRLRDLNREIFMVVLLDSANQIIRDCTISEGT
                     LNASLVHPREVFRAAIEYMAASIILVHNHPSGNPEPSREDIAVTEQLKEAGIIMSIPV
                     RDHIIIAGRHYTSLAKSGYL"
     gene            complement(2836..3609)
                     /locus_tag="PK754_07755"
     CDS             complement(2836..3609)
                     /locus_tag="PK754_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09436.1"
                     /translation="MGNTVKFGDWISRGFDLLRKDWGTWALMGSLYFGPLVVALIMLY
                     ASIFALPFLGKHMDPDVALPGMVMFGLLVLGIGLFMLFLLLGMYLTARKQLKGESISV
                     SDAFSGGGHFFTVLVICLVYVFAACIGFVFCVFPAFIVGGFFYLAIPIAVYEGLGPME
                     AIQKSFSIAKKDWLMFTLFALVAQIIAQVGLYACYIPIVFSIGLQFTFAAVAYEDCVN
                     PAAANDAPTFTGAVKYCTTCGKQLRSETNFCDNCGAAQV"
     gene            complement(3624..4421)
                     /locus_tag="PK754_07760"
     CDS             complement(3624..4421)
                     /locus_tag="PK754_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09437.1"
                     /translation="MNPNYQVRLGKWLSEGWTMLRQEWMAWTGISALTFIPAGFVYIM
                     IQMLIGRSLQIGPNESLTDYFLRSMESSFAVNMLTQFAMVFIIAFFMGGAFRAAFRQI
                     QGESISVSDAFSGVDIYVSVLVAMLSVSLFSMIGSMLCLFPGLIAQGLLYYVVPMVVR
                     ENLPPLEALKKSFETTKSDWVMYTVLWIVTQVLAFLGILLCGIGIFITAPLIVTTTAA
                     AYRDCFEIKTEEPISRVDALYSKSCIRCGASIDVRASFCDKCGASQV"
     gene            complement(4511..5797)
                     /locus_tag="PK754_07765"
     CDS             complement(4511..5797)
                     /locus_tag="PK754_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HNB09438.1"
                     /translation="MFSTVESLGHSQIMFFGIPFEFTLFALTLLGVALFHHYVLQVAL
                     IGLASILTFKLGFTNFNLMNHLHHEWEILTNLLGLLLGFALLAKHFEESKIPEILPKY
                     LPSGWMGGFVLLFIVMILSSFLDNIAAAMIGGTIAMVVFKGNVHIGFLAAIVAASNAG
                     GAGSVVGDTTTTMMWIDGVNALDVTHAFVGSIASFLVFGFIAAKQQHKFQPIVAAHAE
                     DIAIDWGKILVVFLILVGAITTNFMLDFPAVGVWIAILIGATFRKTAWHELPGALKGS
                     IFLLSLVMCASMMPVEELPPASWETAFSLGFVSAVFDNIPLTKLALTQGGYDWGILAF
                     TVGFGGSMIWFGSSAGVALTNMFPKGKSVVFWIKDGWHVAVAYIIGFFIMLYMVGWHP
                     HAPHKEGKETKPHAQVIEQAPAGSCCMPGAGGTSSH"
     gene            complement(5952..6503)
                     /locus_tag="PK754_07770"
     CDS             complement(5952..6503)
                     /locus_tag="PK754_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNB09439.1"
                     /translation="MEPIHKPFRLSDLKPPEIHDSVFIANTASVIGGVKIGENCGIWF
                     GAVLRCDMDPIIIGKNCNIQDNAVVHVDDHFPAIIEDNVSIGHSAIVHGATIRKNCII
                     GMHATILNGAEVGENCIVAAGAIVMQNQIIPPNSVVMGVPAKVKKTADEQTLAMIRRN
                     WEVYCEFAAEYRKSKYHKDKSLL"
     gene            6674..7081
                     /gene="cdd"
                     /locus_tag="PK754_07775"
     CDS             6674..7081
                     /gene="cdd"
                     /locus_tag="PK754_07775"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNB09440.1"
                     /translation="MNEREEIEALIQEALHARSFAVAPYSKFQVGALVVMKSGKRFTG
                     CNVEVSSYGLTICAERTAIFKSLSEGERDVHTVVVVADTTGPCFPCGACRQVLSDFAP
                     NARIICSNIHGVYSVFSMKELLPHAFDANDLHP"
     gene            7126..8214
                     /locus_tag="PK754_07780"
     CDS             7126..8214
                     /locus_tag="PK754_07780"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNB09441.1"
                     /translation="MAEWFGVFQVEDPVWRHLIQAGVIIVGGYVLAKLLYRLVHIIGY
                     KISAKTETDLDDKIVAIFEKSVQRIVNTTALYLAINRLEPLFQGTWLNYVDGAFFVVM
                     VILATILLTSVTKTIIEWYVATIAVRTESPLDEEILPLIRRLLNMLLYSIALMICLDH
                     FHIDIKALVVSLGVGSFAIAFAAQETLSNMIAGFVIMVDRPFRVGDRIRITAGNITGD
                     IVKVGLRSTQILDFDYNIVTVPNAQIVKSDIINFAYPDPAMRLRIEVGIAYGSDIERA
                     KKILLEVLQSDERILKEPLPEVAVTGFGDSAVQLVAIGRVRHYKEHFETGDTVRDRIH
                     SSFARNGIEIPYPHRVIIARTDQNAKNG"
     gene            8376..>9923
                     /locus_tag="PK754_07785"
     CDS             8376..>9923
                     /locus_tag="PK754_07785"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HNB09442.1"
                     /translation="MLKVRLTGIAVWAAVLLSFFAQTAQSQSNARGMFEFVSRVGNPD
                     DGATDLVLDGKTLFVANKFSGLQIIDISDIRRPIIRSRTKSEGQNFGIAKKDKMVFLS
                     DNKAGLVIYDVTSLSKPKLVTSFETRGEAWDVQIKDNYAFVAAGLAGLLVIDITDPKA
                     PKEVGSLRFDKDWDFARQLLIRENRLYLADRKAGVHIINIENPLAPKEIKRFPTQFAE
                     GLDVWDNYICVADGPSGFVILDITNPEKIRQIAAQSLPGYANRIFKEGPYVYIGVDDA
                     GIRAYDVSNPAKPFFDARYDTPGQTFGMIKYDIFLVAADLNAVLFLTHNKPPVITRSG
                     DKVIAENQTLRFKIKGMDPDNNPIGFSANFIPQGASFDIKDTVFSWTPSFEQSGVYEG
                     IVFSATEKTQSALFSRDTIKITVNNVNRAPALPSTGNYTVDENKELAFTINKPSDPDI
                     EDTTNLKVELTNPPTGAIFDSKTLTFKWIPAYDQSGVYTLSFVVTDGGGATDTKQTTL
                     TVKNINRP"
BASE COUNT         2676 a         2279 c         2385 g         2583 t
ORIGIN      
        1 agaaaaaaag cgctggataa aataggcggc tttgataccg gattggaatt ttggagtgaa
       61 gatacgcaga tcggtcgccg tatgagccag gaaggccgtg ttcgtttttt tcaccgggcg
      121 tatgtttata catcggcacg tcgttattat gcagagggat ttcttcgcgt gctcatgcgg
      181 tatatcatga attatttttg ggatattatt tttcatcgcc ctttcaccaa aggttatcag
      241 gacgtacgtt ctaccccgcc gaagcaaacg acttaaaatc ttccaacgtt tacggtgtaa
      301 tttgttttac aaaaaccgat cgcgcttttt cagccggatt agtgtcggga tagtccagaa
      361 ccgccgtatt gatgtatatc tggttggtta tgattttttg gatttcttta cctaccgcaa
      421 aagcgtatag cgctgtatcg gcatccgctt cgatggggat actccactcg atgcgatccc
      481 ctcgtttact cgcgatgtag taaccgtctt tattgatata cacatggcat gcggtagcca
      541 aggtaggatg cattagacgc gccgtgccgc ggaggaacca tctttctttg cgggtgatgg
      601 cgatgtcttt gcgcagcaaa agaacgggcg acgggcttag agcctgtgtg agcgatagga
      661 cgcttttgac agtgtcctga aaaaacaaag catattcttt ggtttgttcg ttactggcgc
      721 gggctagcgt aagaatcata ccgggttcat aggggatttc gatatgtata ggcacactgt
      781 tgccgatcgc gttgacaaac tgaacgcgcg ggccggccgc aacaagcatg ctgcgatccg
      841 attgaaattg cagatccaac acgagaaaat catcgctgcg cttttcgata aataaagaat
      901 ccatcatcag atcgcgttcg caaaaaacct gcgtaccgta atgacgtatc gtataacgcg
      961 cgagacgcgt tttggcgccg cggatcagcg tatctttgat cacactgtct ttgtatgaag
     1021 caataccgat accgatatgc gtaaattccg ctttcaatat attagcgcga tggccgggac
     1081 tattcatcca ctgcttcatc agttgatcgg cgatgctgcg atatccgcca tccacgctat
     1141 gcaaagcaat attttcgccg agcgatgcgt ccgatgcccc cgtgcgatat acgcggtcgg
     1201 atacggtttt tagtgaagcg gtaggggaag tatgagaaaa ataacccagt gccatcattt
     1261 cacgggaatg ttgccgggcc gctgagcgta aggctttatc ccacacgaga ggcggaagtt
     1321 tagctttggt gcgttcaata tttacctgat tccaaacttc catttcggct aaagagtcgc
     1381 ccggtagcgc cgaaaattcg tcatgcaggt attggatctg ttgtccccaa atcgctgtcg
     1441 tcaggcataa tgaaacaaat aaaaataact ttttgatcat gacgaattat gctcctgttg
     1501 aggcgaggcg taaagcctgt ttgatcatat cctgtagagc aaacggctta tcacccgctt
     1561 cacgtaataa gcgagcgatg gctttttcgg caacgggttt ggagtagccg agagagatca
     1621 gtgccattaa ggcttcatcg gcgacctgca gattggccga tgacgtgcta agcttagcgg
     1681 ttgatgcgac gtcgcccgtt ttggtgattt tgtctttgag ttcgacgacg atacgttgtg
     1741 cggttttttt gccgataccc ggaatggccg tcagcaattg tacgtcttcg ttgaggacag
     1801 cggctttaaa atcacggatc gcgatgccgg acatcatact gatgccggac ttggggccga
     1861 tgccggagac ggaaatcaaa agtaaaaaaa acgagcgctc ttcagcgctt acaaagccga
     1921 ataactgcat gatgtcttcg cgcacatgca tatacgtcag tagcttgcaa gccgcgccta
     1981 cggccgggat tttttcgtag gtgctcacgg gtataaaaag ttcgtagccg ataccctgta
     2041 cgtcaataac ggcgcgtgtc ggttcttttt cggccactag gccttggaca aatgtaatca
     2101 tgattaaaga tatcctgatt tggctaaact ggtatagtga cgtcccgcga tgatgatatg
     2161 atcgcgaacg gggatactca taataatgcc ggcttctttg agctgttcgg tgaccgcgat
     2221 atcctcccgg ctcggttccg gatttccgct gggatgatta tgtacgagga taatacttgc
     2281 cgccatatat tcgatcgccg ctcgaaacac ttcgcgggga tgtaccaaac tggcattgag
     2341 tgtgccttcg gagatcgtgc aatcacgtat gatctgattg gcgctgtcca gcaataccac
     2401 cataaaaatt tcccgattaa gatcgcgtaa ccgtggcata tagtgtttga ataaatcctc
     2461 cggcgcacgt acacggattt tttttgcatc gctttgagcc tcgatgcgac gggataattc
     2521 gaaagccgcg gccaatttga tggacttcac tttaccgatg cctttaaaac ggcgcaattc
     2581 gtgcgtcgaa caccgcgcca gtgcggcaag gcctgagaat tccttttcga gcgcacgcgc
     2641 caaatcaacg gctgtaatat tacgcgtacc gctgccgatg agtatagcta ttaattcggc
     2701 ggaactcaat cgtgcggcac cgagttgttc taatttttcc cgcggtcggt cttgcgaaga
     2761 ccagtctttg atcttggcat gatagagcga aggttcggct attttttttg gtttacccac
     2821 gatggtttcg attgcctata cttgcgctgc gccgcaattg tcgcaaaaat tggtttccga
     2881 acgaagttgt tttccgcacg tcgtacaata tttaaccgcg ccggtaaagg taggcgcatc
     2941 attggcggct gccggattta cgcaatcttc ataggccact gcggcaaatg taaactgcaa
     3001 gccgattgaa aaaacgatcg gtatatagca cgcatataaa ccgacttgtg cgatgatttg
     3061 tgcaaccaaa gcaaagagtg taaacataag ccaatctttt ttggctatgg aaaaactttt
     3121 ttgaatagct tccataggcc ccaggccttc gtatacagcg atgggtatgg ccaaataaaa
     3181 aaaaccgccg acgataaaag ccggaaaaac gcaaaaaacg aatccgatgc acgcggcgaa
     3241 aacatataca agacaaataa ctaatactgt gaaaaaatgg ccgccaccac taaaggcatc
     3301 cgatacggag atggactccc cttttaattg tttacgcgcg gtgaggtaca tgcccagtaa
     3361 aaggaaaagc ataaaaaggc cgattcccaa aacgagaagg ccgaacatta ccatacccgg
     3421 caaagccaca tccggatcca tgtgttttcc gagaaagggg agtgcgaaaa tgctggcata
     3481 caacatgatg agtgcgacga ctagcgggcc gaaataaaga gatcccatta gagcccacgt
     3541 accccagtct ttgcgcagca aatcaaatcc gcgcgaaatc caatcgccaa attttaccgt
     3601 gtttcccata catcctttct cagttatact tgtgatgcgc cgcacttatc gcaaaacgat
     3661 gcgcggacat ctatggaagc tccgcatcga atacaggact ttgagtataa tgcgtccacg
     3721 cgagatatcg gttcttccgt tttgatttca aaacagtctc tgtatgctgc ggctgtcgtg
     3781 gtcactatca aaggggccgt aataaaaata ccgattccgc acaatagaat gccaagaaac
     3841 gccagcacct gcgttacgat ccaaaggacg gtgtacatga cccaatcgct tttggtcgtt
     3901 tcaaaacttt ttttcaaggc ttccaatggc ggaagatttt cgcgaacaac catcggtacg
     3961 acatagtaca aaagtccttg tgcgatcaaa ccgggaaata gacacaacat gctgccgatc
     4021 atgctaaaca aactgactga aagcattgcg actaataccg atacataaat atccacgccg
     4081 gagaaagcat cgctaacgga aatggattcg ccctgaattt ggcgaaatgc cgcccgaaaa
     4141 gcgccgccca taaaaaaagc gataataaat accatggcaa attgcgtgag catattgacg
     4201 gcaaacgacg attccatgga gcgaagaaaa taatccgtca ggctttcgtt agggccgatc
     4261 tgtagcgaac ggccgatcag catttggatc atgatataga caaatccggc cggaataaaa
     4321 gtcaatgcac tgataccggt ccatgccatc cattcttgac gcagcatcgt ccaaccttcg
     4381 ctcagccatt tgcccaaacg tacttgataa ttcggattca tagttcctca aaaaaataaa
     4441 aagcgggttg cgattaacaa cccgcataat aatcaattcc tactttttac aaaagcaaaa
     4501 aaccaaacta tcagtgcgat gaagtgccgc cggcaccggg catacagcaa gacccggctg
     4561 gcgcttgttc gatcacttgg gcgtggggtt tggtctcttt gccttcctta tgcggtgcgt
     4621 gcggatgcca tccgaccata tacagcatta taaaaaagcc gatgatatag gctacggcaa
     4681 cgtgccatcc gtctttgatc caaaatacta cggatttgcc tttggggaac atattggtca
     4741 atgcgacgcc ggccgacgat ccgaaccaaa tcatggaacc gccgaaacct actgtaaatg
     4801 ccaaaatacc ccaatcgtag ccgccttgtg tcagagccaa tttggtcagc ggaatattat
     4861 caaatacagc agaaacaaaa ccaaggctga aggctgtttc ccaggaagcc ggaggcagtt
     4921 cctcgacggg catcatcgaa gcgcacatca ccaaggataa taggaaaata ctacctttca
     4981 aagcaccggg caattcatgc catgcagttt tgcgaaatgt cgcgccaatt aaaatcgcga
     5041 tccatacgcc gacggcgggg aaatccaaca taaagttggt tgtaatcgca ccgacgagga
     5101 taaggaagac aactaaaatt ttcccccagt caatggcgat atcttcggca tgggccgcga
     5161 cgataggttg aaatttatgt tgttgtttgg cggcgataaa accgaaaact aaaaacgaag
     5221 caatcgaacc gacaaaggca tgcgtaacat ccaatgcatt tacgccgtct atccacatca
     5281 tcgttgtcgt cgtatcgccg acgacggatc cggcaccacc ggcattgctt gcagcaacga
     5341 tcgccgcaag aaatccgata tgcacattgc ctttgaaaac gaccatagcg atggtgccgc
     5401 cgatcattgc ggccgcgata ttatctaaaa acgacgaaag tatcatcacg ataaataata
     5461 aaacaaaacc gcccatccaa cccgaaggca gatatttggg aagaatttcg gggattttgg
     5521 attcttcaaa gtgtttggcc aaaagtgcaa atccaagcag caaaccgagc agattggtca
     5581 agatttccca ttcgtgatga agatgattca tcaaattaaa attggtaaac ccaagtttga
     5641 aagttaagat ggaagccaag ccgataagcg cgacttgtaa aacgtagtgg tgaaaaagcg
     5701 ccacaccaag cagcgtcaaa gcaaaaaggg taaattcgaa aggaataccg aaaaacataa
     5761 tttgactgtg accaagggat tcaaccgtgg aaaacatcgg ttgactggaa aacagcgaca
     5821 tcatgccgat cacggccaaa acaaaaagac cgataataac cggcgcggta aaaaaaccgc
     5881 ctttttgtgt ttcactcata gacgctcctc ctgtgtaggg ttaaacaatt tttcgttcgc
     5941 aaaacggtat attacaataa cgatttgtct ttatggtatt tggatttgcg atactcggcg
     6001 gcaaattcac aatatacttc ccaattgcgg cgtatcatcg ccaatgtttg ttcatcggct
     6061 gtttttttga ctttggcagg cacacccatg acaaccgagt tgggtggaat aatctgattt
     6121 tgcatgacga tcgcacccgc cgcgacaatg cagttttcac cgacttcggc accgttgagt
     6181 atcgtcgcat gcatgccgat gatgcagttt ttgcgaatgg ttgcgccgtg cacgatggcg
     6241 ctgtgaccta tagaaacatt gtcttcgata atggcgggaa aatgatcgtc cacatgcacg
     6301 acggcgttgt cctgtatatt gcagtttttt ccgatgatga tggggtccat gtcgcaacgt
     6361 aaaacggcgc caaaccagat gccgcaattt tcgccgattt ttacgccgcc gatgacagaa
     6421 gcggtattgg cgataaaaac gctgtcgtga atttcaggcg gtttaagatc agacaaacga
     6481 aaaggtttat ggatgggttc cacgatgcct cggtgtacac aatgtttaaa cggtggttgg
     6541 gatgttgatt cagcggcggt aagttctaaa aaaaaagatt caaagtcaaa tcgcgagcct
     6601 taaccactct cagcttatcc gggtatctcg gaagttttct tgcacttatc ggccgcagag
     6661 cgtataatct gttatgaatg aacgtgaaga aatagaagcg ctgatccaag aagcgctgca
     6721 tgcgcgatca tttgccgtgg cgccatattc caaatttcag gtcggcgctt tggtggtaat
     6781 gaagtccggc aaacgattca ccggatgtaa tgtcgaggtg agttcgtacg gtctcacgat
     6841 ctgtgccgag cgcacggcga tttttaagtc gctttcggaa ggcgaacggg atgtgcatac
     6901 cgtcgtggtt gttgccgata cgaccgggcc gtgttttcct tgcggggcgt gccgtcaggt
     6961 actttccgat tttgcaccca atgcgcggat aatttgcagt aatattcatg gtgtgtattc
     7021 cgttttcagc atgaaagaac ttttgcctca cgcttttgat gcaaacgatc tccatccgta
     7081 aatttattta atgtttaaat ttttcaacta aaggtctgtt taaaaatggc agaatggttt
     7141 ggtgtatttc aggtcgaaga tccggtatgg cggcatttga tacaagccgg tgtgataatt
     7201 gtcggcgggt atgtattggc taagcttttg tatcgtttgg ttcatattat tggctacaaa
     7261 atttccgcta aaacagaaac ggatctcgac gataaaatcg ttgccatttt tgaaaaatcc
     7321 gtacaacgga tcgtcaatac gacggcgctt tatttagcta tcaatcggtt agaacctcta
     7381 ttccaaggta cgtggcttaa ctacgttgac ggcgcttttt ttgtcgtgat ggtcattctc
     7441 gcgaccatac tcctgaccag tgtaactaaa acaatcatcg aatggtatgt ggctaccatc
     7501 gcggtgagga cggagagtcc gctggatgaa gaaatacttc cgctgatccg tcgtcttctt
     7561 aatatgttac tttattctat cgcactgatg atctgtcttg atcattttca tatcgacatc
     7621 aaagcgcttg tcgtgtcgct tggtgtgggt tcgtttgcca ttgcgtttgc ggcgcaggaa
     7681 acgttgtcga atatgatcgc gggctttgtg atcatggtgg atcgcccgtt tcgcgtgggg
     7741 gatcgtattc gtattacggc cggcaatatc accggcgata tcgtcaaagt cggtcttcgc
     7801 agcactcaga ttcttgattt tgattacaat atagtcaccg tgcccaacgc acagattgtc
     7861 aaatcagata ttatcaattt tgcgtatccg gatcccgcga tgcggttgcg tatcgaagtg
     7921 ggcatagcct acggcagcga catcgaaagg gcgaaaaaaa tattgctgga ggtattgcaa
     7981 agcgatgagc gcatcctcaa agaaccgttg cctgaagtgg ctgtgacagg ttttggcgat
     8041 tcggctgtgc aactggtggc gatcgggcgc gttcggcatt ataaagaaca tttcgagacc
     8101 ggcgacacgg ttcgggaccg gatccattca tcatttgccc gcaacggcat tgagatacct
     8161 tatccgcatc gggtgattat tgcccgtacc gatcaaaacg caaaaaacgg ctgatttttc
     8221 agggtatgat tttataaaaa aacgagtttt tttgaattgc ttaacttgct tttttaggcg
     8281 tacctgtcta tattcgcggg cgtaaccctg cgttcaatta tctgcataat tgtggtaact
     8341 ttcgatagag taaaatgaat ttcccggaga aaacaatgct caaagtacgt ttaaccggta
     8401 ttgcggtttg ggccgcagtg cttctgtctt tttttgcaca aacggcacaa agccaatcca
     8461 atgcacgcgg catgttcgaa tttgtgtccc gcgtgggaaa tcccgacgac ggcgctacag
     8521 acctcgtttt ggacggaaaa acgctttttg ttgccaataa attcagcggc cttcagatca
     8581 ttgatatttc ggatatccgc cgcccgatta ttcgcagccg taccaaatcg gaaggtcaga
     8641 attttggtat cgccaaaaaa gacaaaatgg tgtttttatc ggataataaa gccggtttgg
     8701 ttatctatga tgttaccagt ttgtccaaac ctaaactcgt gacatccttt gaaacgcgcg
     8761 gtgaagcgtg ggacgtccag atcaaagata attatgcttt tgtagcggcc gggcttgccg
     8821 gtcttctcgt tattgacatt accgatccca aagcgcctaa agaagtcggt tctttgcgtt
     8881 ttgataaaga ttgggacttt gcgcgtcagc ttttgattcg tgaaaaccgc ttgtaccttg
     8941 ctgatcgtaa agccggcgta catatcatca atatcgaaaa ccctcttgcg cccaaagaga
     9001 tcaaacgttt tcctacgcag tttgccgaag gtttggatgt atgggataac tacatctgtg
     9061 tggcggacgg gccgagcggc tttgtgattt tggatatcac caatcccgaa aaaatcagac
     9121 aaatcgcagc gcaatctttg cccggttatg ctaaccgtat cttcaaagaa gggccgtatg
     9181 tttacatcgg tgttgacgat gcgggtattc gcgcttatga tgtgagcaat ccggccaaac
     9241 cgttttttga tgcacgttac gatacgccgg gtcaaacgtt cggtatgatc aaatatgata
     9301 ttttccttgt tgcggccgat ttgaatgcgg ttctttttct cacgcacaac aagccgccgg
     9361 ttattacgcg ttccggcgat aaggtcatag ccgaaaacca gacgttgcgt tttaaaatca
     9421 aaggtatgga tccggataac aatccgatcg gtttttctgc caattttatt ccacaaggtg
     9481 catcgtttga tatcaaagat actgtttttt cgtggacacc ttctttcgaa caatccggcg
     9541 tatatgaggg gatagtattc tccgcgaccg aaaagacgca atcggcttta ttttcgcgtg
     9601 atacgatcaa aatcactgta aataatgtca atcgcgctcc ggcgcttcct tccacgggaa
     9661 attacacggt agatgaaaat aaagaattgg cttttacgat caataagccg tccgatccgg
     9721 atatcgaaga tacgacaaac ctcaaagtcg aattgactaa tccgccgacc ggtgcgatat
     9781 ttgacagtaa gacgctgact ttcaaatgga ttcccgctta cgatcaatcc ggtgtataca
     9841 cattgtcttt tgtggtgaca gacggtggcg gcgcgacgga tacaaaacaa acaacgttga
     9901 cggttaaaaa catcaatcgc cct
//
LOCUS       DAOQNQ010000101         9894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2490_length_9894_cov_5.402378, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000101 DAOQNQ010000000
VERSION     DAOQNQ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9894
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2490_length_9894_cov_5.402378"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PK754_07790"
     CDS             <1..284
                     /locus_tag="PK754_07790"
                     /inference="COORDINATES: protein motif:HMM:NF037895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09443.1"
                     /translation="LPASGTSVRSRNLENDPPLVLKPGMKQQAVYKGVLYGARVDQSG
                     RIRYGGKLFPSPSTAGSFIRSGKSTNGWVFWKYQTPDGHWRYIDELRNR"
     gene            317..1789
                     /locus_tag="PK754_07795"
     CDS             317..1789
                     /locus_tag="PK754_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09444.1"
                     /translation="MEFVLAHYIHRTIPMKFLIYGVLFCCFGVLYADTLSGSEVDSAV
                     IRFRQLVREHRIIQLSRLIKYPLRRTSPIPDIQNPYAFIRYFPVLFDSSITKRLNASG
                     NDDLVWRTDHYEMVDGRIWLDSNGYIVAIPYVSSHEEHLISQIKAEACAADITDSVFA
                     RRTRLFWDADAYIIRVDETAEGLRYSSWNRGKSVTQSPDLMLWRGSVSNDSSRFIFIQ
                     GVWRYVLTTEFSPDEAKPQKHFLEVWKADTLKMRLPCEELAHAQQISRFCETIHHIRN
                     DRIVTLSRGVRFPFGSLMDMLPPEENPDEFILSYPLMVDSMVHRRFNNYHPVSIAHKY
                     GYMWEDLFFDDNCRLTLLGYKAALADSIFLWLQREVTDALYSELREFGSHDMMLISDN
                     HVLRIDWMDEQRGWRYVAWRKGKAISDVPDIILYGGTAEYHGNMGSKTLTFTNGTWSY
                     IIDYWVNDYLNPTLIIKNNAQEIINTKCQRITFSDCKLYH"
     gene            complement(1891..2442)
                     /locus_tag="PK754_07800"
     CDS             complement(1891..2442)
                     /locus_tag="PK754_07800"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB09445.1"
                     /translation="MNDFFEQTQARYTAILERLDRDIRSFSDAQIHWKADANTWSIAE
                     ALQHIITADTAYLEPMHRALQGAERTASPVAYRPSFFARLFIYSLRPGRFRYKAPAVF
                     KPESSRISHTIRQNFADHIRTQMQALQAWRSYDINRLKMTSPANAMVRISLGEALDVM
                     ITHTERHMAQVDRILQHPQFPTL"
     gene            2717..3262
                     /locus_tag="PK754_07805"
     CDS             2717..3262
                     /locus_tag="PK754_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09446.1"
                     /translation="MKKILNIYFLFGALYTLAYAGDGVTNEFVATAGWGAPVGIGLEF
                     EKYIVPAFNLNMGAGVTVTGVKLSVGTKYLMAADKDVSPYIGLNFSSSSGIRRLNINV
                     DTDTAKYKINAAKILTPRAGLKLKRGFANFYLNVGYGIVVDGGGNKYRSGSTDTGISD
                     MADLLSAGGVEISGSICFPIK"
     gene            3353..3952
                     /locus_tag="PK754_07810"
     CDS             3353..3952
                     /locus_tag="PK754_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09447.1"
                     /translation="MRVMLTVFVILLVLSDILSAQVSYLQNNESNAVALSAGASIGSK
                     NNKSRALGFETGQRKFVFGVSYSETKPQSEDKYDAVSAHIGTTFPHQIDGMNLSLRLG
                     YLGSSKIKIPAKHVVFSPSVFLNIAHKITADSFIKIIPEAGVSRLFVFEEQYEGDLVS
                     SVGVTILYNRPTMAFGLGLSRNWSSGPVVNLLSFSLITD"
     gene            4068..5429
                     /locus_tag="PK754_07815"
     CDS             4068..5429
                     /locus_tag="PK754_07815"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNB09448.1"
                     /translation="MNRIQLDYTYVMPFLKEEEIFGMQAKVNDVHKMIEQRTGAGNDF
                     LGWLDLASKFPSALVEDIAKTAEDIRKTSDILITIGIGGSYLGAKAVIDALTHSFNNN
                     IPHKTPQVYFAGQNMSGRYLRDLTEVIQNKRVAVNVISKSGTTTEPALALRYFRKIMS
                     DAMSKMQVSQRIFATTDAARGSLKKLAEKEGYKCYVIPDDVGGRYSVLTPVGLLPIAV
                     AGVDIRALLQGAADMEKILSNPDLKSNPAYVYAVIRNLMYNKGKAIEILANFEPSLHY
                     VSEWWKQLYGESEGKDHKSIYPASVDFSTDLHSMGQWVQQGTRNIYETFLWVDKAQSD
                     TSVPDLEGDVDGFNFLTGQTFEYVNKKAYEGVTLAHRDGEVPNMTVHLPELNAYYLGQ
                     MIFFFEKACAMSGYLLGVNPFDQPGVEAYKTNMFALLGKKGYEEKSKELNAVLGQMQR
                     KKI"
     gene            5435..5758
                     /locus_tag="PK754_07820"
     CDS             5435..5758
                     /locus_tag="PK754_07820"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB09449.1"
                     /translation="MMTRSEALRAILEKDPGDSFSRYALAMELVGAQRWAEAIAEFTD
                     LQRRDPSYVALYYQLGKCYEQNNQPQDAIAAYTEGIRVARQANNMHAVSELYAAKEEL
                     EDTLA"
     gene            5766..7355
                     /gene="rsmB"
                     /locus_tag="PK754_07825"
     CDS             5766..7355
                     /gene="rsmB"
                     /locus_tag="PK754_07825"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:TIGR00563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HNB09450.1"
                     /translation="MPFSKKPGARSGAKRTSDKRTSDKRTSDKRTSENRTSEKRTFAG
                     KERGERYSDERPKPRRRPKYYQPRTFMGANSETEERRLAEWTGQRIALEVLHAFEETQ
                     ERADILLRTHIQRHLGILDSRDREQIARTALNVIRYKLRLDYVVSKTLKRNAEELEPL
                     IRCIYRLAAFHIVHLGHSVERTQAIVNFIVPKSYGNQKENLRNFISYLKKYHTEISFP
                     NPRKQPVEALAITYSHPSWLIEKWSKTYGTESTVRMCEFDNQETPLVLRINTLKATEA
                     DVFKKLKAEGIIPKKTAYAQYGLIPGEVHDIFNRKIFKDGWVEVQAESSQIFCEWVNP
                     ASEDRVLDACAGGGGKSLALSMLMHNTGHIVAADINAGALEQLKKRATRAGCKNIEVI
                     ENDALAALNQRGTWYDKIIIDAPCSGLGVLQRNPDMRWRVTQDNIEKLVNEQRTLLEN
                     YAPLVKPGGMLIYATCTLSEEENENVIEAFLSAHPEFELQKNHDARWKKFISGKGYFC
                     ITPFEHNISGFFSGKLIRRKN"
     gene            7374..8528
                     /locus_tag="PK754_07830"
     CDS             7374..8528
                     /locus_tag="PK754_07830"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNB09451.1"
                     /translation="MKILVLGAGWMGRAVVFDLLRNHHMERIIVADANAQALKETEAF
                     VKAENDLRFLAKKINVEDEEALKKLMSQVDTVVSAVTYKYNYKLARLAVQCGCNFCDL
                     GGNNTIVAQELSLHDKAKKKNITIIPDCGLAPGMVSVLVADGIGQMDETTDVQIRVGG
                     LPLEPRPPMNYKLVFSVHGLINEYVEPCVKIQSGEVVTVDPMVDVEELEFPAPFGKLE
                     AFNTSGGTSTLPQTYLGKVKNLDYKTIRYPGHAAQFKLLMDLGLTESKPVKVGNDTVV
                     PRDLLAQNLTKVLTMEGRDAVLVRVTVKGIKGGNPKEIQYQIVDYGDASNNITAMMRL
                     TSYPISVIAQMMTEGKITEKGAVPQELAVPTRSFIEALRARNIDIQITES"
     gene            8893..9237
                     /locus_tag="PK754_07835"
     CDS             8893..9237
                     /locus_tag="PK754_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09452.1"
                     /translation="MRRTTSAARKTKQNPVLMDLENYILKLGLNSEPTSANVVAKRLE
                     LKESPKNIQMIKQALEKMVREKKVYRCGSRYAIDYQGGFNLMKTFCIKVAEENYKINN
                     FVFEGVAYARGA"
     gene            9277..>9894
                     /locus_tag="PK754_07840"
     CDS             9277..>9894
                     /locus_tag="PK754_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HNB09453.1"
                     /translation="MTSVRRRFFFKVACFYFRPYLFKKTTEEDAFMQELIRVESLCKS
                     YPVGSTLWGRATWSQVVEDVSFIVRTGETLGVVGESGSGKSTIGRCLLKLTDPTSGRI
                     FFDGEEITALDNKAMRPFRRRMQIVFQDPFSSLNPRQTVDAMFREIFTVHATASGTEA
                     TDKIEELLKTVGLDPSQRFCFPHEFSGGQRQRIGIARALSVRPQFI"
BASE COUNT         2716 a         2174 c         2437 g         2567 t
ORIGIN      
        1 gcctgcccgc gtccggtaca tccgtacgtt ctcgcaattt ggaaaatgac ccgccacttg
       61 tgctcaaacc gggtatgaaa cagcaggccg tttataaagg cgtgctgtat ggcgcacgcg
      121 tggatcaaag cggtcgaata cgttatggcg gaaaactgtt tccttcgcca tccacggccg
      181 gttcgtttat tcgcagcggc aaaagtacca acgggtgggt gttttggaag tatcaaacac
      241 cggacggcca ctggcgatac attgatgaat taagaaaccg ttagaaaatc agataattat
      301 ctcttatgca tacatcatgg aattcgttct cgcgcattac atccatcgca ctatccctat
      361 gaaatttttg atttacggtg tattattttg ttgtttcggt gttttatatg ccgatacgtt
      421 atcgggttcg gaagtggata gcgctgtaat ccgatttaga caattggtac gcgagcatcg
      481 tattattcag ttgtcccggc ttataaaata cccattgcga cgcacatctc cgatacccga
      541 tattcaaaac ccgtatgctt ttattcgtta tttccctgtt ttgtttgata gttcgatcac
      601 aaagcgtctt aatgcttccg gtaacgacga cttagtttgg cgaacggatc attatgaaat
      661 ggttgacggg aggatttggc tggatagcaa tggatatatc gtagccatac cttatgtgtc
      721 atcacatgaa gaacatttga tctcccaaat caaagctgag gcatgtgccg ccgatatcac
      781 agatagcgtg ttcgctagac ggacgaggct tttttgggat gcggatgctt atattattcg
      841 tgttgatgaa acggcggaag gacttcgcta ttcgtcatgg aatcgcggaa aaagcgtaac
      901 ccaatcacct gatcttatgc tttggcgcgg tagcgtttca aacgattcca gtcggttcat
      961 cttcatccaa ggcgtttggc gatatgtact tacaaccgaa ttctcgcccg atgaagctaa
     1021 accgcaaaag cattttctag aagtttggaa agccgatact ctcaaaatgc gattgccttg
     1081 tgaggaactc gcgcatgcgc agcagatcag tcgtttttgt gagacgatcc atcatatacg
     1141 taatgatcgc attgtgacat tgagtcgtgg cgtgcgtttc ccttttggat cattaatgga
     1201 tatgctgccc ccggaggaaa atcccgatga atttatattg agttatccgt taatggttga
     1261 ttccatggtt catcggcgtt ttaataacta tcatcctgtc tcgatagcgc acaaatacgg
     1321 atatatgtgg gaagatttgt tttttgatga taattgtagg cttactctac tcggatacaa
     1381 ggcggcactt gctgattcca tatttttatg gctccaacgt gaagtgacgg acgcgctcta
     1441 ttccgagctt cgggaatttg gtagccacga catgatgttg atttcagata atcacgtact
     1501 tcgaattgat tggatggacg aacagagagg atggcggtat gtggcgtggc gtaaaggtaa
     1561 agctatttca gatgttccag acattatact atatggtggc acggctgagt atcatggtaa
     1621 tatgggcagt aagacgctca cgtttaccaa cggcacgtgg tcatacatca ttgactattg
     1681 ggtaaacgac tatctgaatc caacgcttat aatcaaaaat aatgcgcaag aaattattaa
     1741 tacaaaatgc caacgtatta cattctcaga ttgcaaattg tatcattaat ggactaaacg
     1801 ggaaaagcgc caaggcatag acaaaaaacc aaatcgcacg cacccactca atcaatcccg
     1861 ttattgttca gccgcacgta aatccaaatt tcataatgtc ggaaactggg gatgctgtag
     1921 gatgcgatcc acctgcgcca tgtggcgttc ggtatgggtg atcatcacat ccagcgcttc
     1981 accgagactg atacgcacca tcgcattggc gggggatgtc attttgaggc gattgatgtc
     2041 gtacgaacgc catgcctgta acgcctgcat ctgtgtgcgg atgtgatcgg cgaaattttg
     2101 acgaatggta tggcttatgc ggcttgattc cggtttgaaa accgcagggg ctttgtatcg
     2161 gaaacgaccc ggcctcaggg aatagataaa taaacgtgca aaaaaactcg gacggtaggc
     2221 gaccggcgaa gccgtacgtt cggcgccttg caaagcacgg tgcataggct caaggtaggc
     2281 ggtgtccgcg gtgatgatat gctgcagcgc ctctgcgata gaccatgtat tcgcgtcggc
     2341 tttccaatgg atctgcgcat ccgaaaaaga ccggatgtcg cggtcgagcc gttctaatat
     2401 cgccgtatag cgggcctgcg tttgttcaaa aaaatcgttc ataaaagatt aattatttcg
     2461 tgttacggtt gatcggcgcg aaaaatatat tacatgttgg cgaaaatcaa tatgcaataa
     2521 atagagatag ttgtttcgat tgaattatca atcatggact caatcgtata agtacgaact
     2581 gcaacttacg aagtgttgat gtgtgagtca taaggcgaaa gaaaatcatg gaaaatcact
     2641 atggttttta tgccaaattt tgggtatgat gatatgatct gttttatatc aatcatcttt
     2701 attctggggt aaaacaatga aaaaaattct gaatatctat tttttgtttg gcgctttgta
     2761 caccttggcg tatgccggag atggtgtgac gaatgagttt gtagcgacgg caggatgggg
     2821 cgctccggtg ggtatcggct tggagttcga aaaatatatt gttccggcat tcaatctcaa
     2881 tatgggagcc ggtgttacgg taaccggggt gaagctgagt gtaggtacca agtacttgat
     2941 ggccgccgat aaagacgtat cgccatacat cggtcttaat ttctcgagtt cttcgggaat
     3001 acgtcggcta aacataaatg ttgatacgga tacggctaaa tataaaataa atgcggccaa
     3061 aattttgaca ccacgggcag ggttaaagtt aaaaagagga tttgctaatt tttatctcaa
     3121 cgtaggctat ggtatcgtcg tggatggcgg aggtaataag tacaggtcag gaagtacaga
     3181 taccggcata tccgatatgg cggatcttct gtccgcgggc ggcgtggaaa tcagcggcag
     3241 tatttgtttt ccgatcaaat gacgaattga cttgctgtcc gacgttgatc gcatgatgag
     3301 ctcatttaat catcagatgc caaaattttt ttgcactaag ttgaaactct gtatgcgtgt
     3361 aatgctcact gtattcgtta ttttattagt tttatcggat attctgtccg ctcaggttag
     3421 ctacttgcag aacaacgaat cgaatgccgt tgcactcagt gccggtgcgt ccatcggcag
     3481 caaaaacaac aagagtcgcg cgttgggttt tgaaaccgga caaagaaagt ttgtgtttgg
     3541 agtttcctac tctgaaacaa agccccaatc agaagacaaa tacgatgccg taagcgcaca
     3601 tataggtaca acctttccgc accaaatcga tgggatgaat ctgagtctgc gattgggata
     3661 tttgggtagc tccaagatca agattccggc caaacatgta gtgttttctc cgtcggtctt
     3721 tctcaatatt gcacacaaaa ttaccgcaga ttcgttcatc aaaattatac ctgaagccgg
     3781 agtttcgcgg ttattcgttt ttgaagaaca atatgaaggc gatctggtga gcagtgtagg
     3841 tgttactatt ttgtataata gacctacgat ggcgttcgga ttgggattgt cgaggaactg
     3901 gtcatccggt cccgtcgtca atctcttatc attcagtttg attaccgatt gacgtagccg
     3961 cttggtctgc catttttcag agatataggg tctccatttg atttctccga ttcagagcgt
     4021 atattacccc cagtcctcaa aaaataccac aaaccggagc acgttttatg aaccgtatcc
     4081 agcttgatta cacgtatgtg atgccgttcc tgaaggaaga agagattttc gggatgcagg
     4141 ccaaagtcaa cgacgtacat aaaatgatcg agcaacgcac cggagcgggt aatgatttct
     4201 taggttggct ggatctggca tccaagtttc cctccgcgct tgtagaggac atcgctaaaa
     4261 cggcggaaga tattcgtaaa acttcagata tccttatcac catcggcatc ggcggttcat
     4321 acctcggcgc gaaggccgtt attgatgcgc tgacgcatag ttttaataac aacatccctc
     4381 acaaaacgcc gcaggtttat tttgccggcc aaaatatgag cggacgttat ctacgcgacc
     4441 ttaccgaagt gatccaaaac aaacgcgttg cggtcaacgt gatttccaaa tccggtacga
     4501 cgaccgaacc ggctcttgcc ctacggtatt ttcgtaaaat catgagcgat gccatgtcca
     4561 aaatgcaggt atcgcagcga atttttgcga cgaccgatgc cgcccgcggg tcactcaaaa
     4621 aattagccga aaaagaaggg tacaaatgtt atgtcattcc cgatgacgtc ggcggacgtt
     4681 attcggtatt gacgccggtc gggcttttgc cgatcgctgt cgccggcgtg gatatccgtg
     4741 cgcttttgca aggggcggcc gacatggaaa aaatactgag caacccggat ttgaaatcca
     4801 atccggcgta tgtctatgcc gtgattcgca atctgatgta caacaaagga aaagccatcg
     4861 aaattctggc caatttcgaa ccgtcgctgc attatgtgag tgagtggtgg aagcagcttt
     4921 acggcgaaag cgaaggtaaa gatcacaaaa gtatttaccc agcttcggtt gatttttcga
     4981 cggatctaca ttcgatgggc caatgggtgc aacaaggcac acgcaatatc tatgaaacgt
     5041 ttttgtgggt tgacaaagca caatcggata cctcggtgcc cgacctggaa ggcgatgtgg
     5101 acggatttaa cttcctcacc ggtcagacgt ttgaatacgt gaataaaaaa gcctacgaag
     5161 gcgtgacact ggcgcatcgc gacggcgaag tgccgaatat gaccgtgcac ttgccggaac
     5221 tcaacgcgta ttatctcggc cagatgattt tctttttcga aaaagcctgc gcgatgagcg
     5281 gttatctgct gggcgtcaat ccttttgatc aaccgggcgt cgaagcgtat aaaaccaata
     5341 tgtttgcatt gctcggcaaa aaagggtatg aggaaaaatc aaaagaactc aatgccgtct
     5401 taggccaaat gcagcgtaaa aaaatctgag tacgatgatg acacgatcgg aagccttacg
     5461 cgctattctg gaaaaagatc ccggtgattc gttttcgcgt tacgcgctgg cgatggagtt
     5521 agtcggcgca cagcgatggg ccgaagcgat cgctgagttt acggatttac aacggcgtga
     5581 tccgtcctac gtcgcgctgt attatcaact cggcaaatgt tacgaacaaa ataatcagcc
     5641 gcaggacgcc atagccgcat atacggaggg catccgcgtt gcgcgacaag ccaataatat
     5701 gcatgccgtg tcggaattgt atgcggccaa agaagaactc gaagatactt tagcttaagg
     5761 aagccatgcc tttttccaaa aaacccggcg cccgatctgg cgcgaaacgc acttcggata
     5821 aacgtacgtc cgataaacgt acatcggata agcgtacctc tgaaaatcgt acatctgaaa
     5881 aacgtacgtt tgccggtaaa gaacgcggcg aaagatatag tgacgaacgt cctaaaccac
     5941 gccggcgacc taaatactat caaccgcgca cgtttatggg tgccaattct gaaacggaag
     6001 aaaggcgtct cgccgagtgg acgggccagc ggatcgccct cgaagtattg catgcattcg
     6061 aagagacaca ggagcgtgcc gatattctgc ttcgcacgca tattcagcgc catctcggca
     6121 ttttggattc gcgcgatcgc gaacagatcg cacgcacggc gctcaatgtg atccggtata
     6181 aattacgttt ggattatgtg gtttccaaaa cgctgaaacg caatgcggag gaattggagc
     6241 cgctgattcg ctgtatctac cgcctcgccg cgtttcatat cgttcacctc ggacactcgg
     6301 tcgaacgtac gcaggcgatt gtcaatttta tagttcccaa atcgtacggc aaccaaaaag
     6361 aaaacctccg taattttatc agttacctga aaaaatatca cacggaaatt tcttttccca
     6421 atccgcgcaa acaacctgta gaagcgctgg cgatcacgta ttcgcatccg tcatggctta
     6481 tcgaaaaatg gagcaagacg tacggcaccg agtcgaccgt tcggatgtgc gaatttgata
     6541 atcaggagac gcctttggtc ttgcgcatca atacccttaa ggcgacggaa gcggatgtat
     6601 ttaaaaaatt aaaagccgaa gggattattc ccaaaaaaac cgcatacgca caatacggac
     6661 ttattcccgg cgaagtgcat gatattttta atcggaaaat atttaaagac ggatgggtcg
     6721 aagtgcaggc cgaatccagt cagatttttt gcgaatgggt caatccggct tccgaggatc
     6781 gcgtgctgga tgcatgcgcc gggggcggcg gaaaatcgct ggcgctttcg atgctgatgc
     6841 ataacaccgg tcatatcgtc gcggcggata tcaatgcggg tgctttggaa caattaaaaa
     6901 aacgcgcaac ccgtgccggc tgtaaaaata tcgaggtgat cgaaaacgat gcgttggcgg
     6961 cgctaaatca acgggggaca tggtacgaca aaatcatcat tgatgcgcca tgcagcggtc
     7021 tcggtgtatt gcagcgcaat cccgatatgc gctggcgcgt tacgcaggat aatatcgaaa
     7081 aactggtcaa cgaacagcgg acgttattgg aaaattacgc gccgttggtc aaacccggcg
     7141 gcatgctgat ctatgcaaca tgtacattat ccgaagaaga aaacgaaaac gtcatcgaag
     7201 cgtttttgtc cgcgcatccg gaatttgaac ttcagaaaaa tcatgatgcc cgttggaaaa
     7261 aatttatttc cgggaaaggc tatttttgca tcacgccttt cgaacataat atatccggat
     7321 ttttttcagg caaactgata cggcgaaaaa actgacagct taaaaggagc attatgaaaa
     7381 tattagtatt gggcgcagga tggatgggac gtgcggtggt gtttgatctt ttacgcaatc
     7441 accacatgga acgcattatc gtagccgatg ccaatgccca ggcactcaaa gaaaccgaag
     7501 cgtttgttaa agcggaaaac gatctccgtt ttttagccaa aaaaatcaac gtcgaagacg
     7561 aagaggcttt gaaaaaactg atgagtcagg ttgataccgt ggtcagcgcc gtgacgtata
     7621 aatataatta caagctggca aggctcgccg tccaatgcgg atgcaatttt tgcgacctcg
     7681 gcggtaataa taccatcgtt gcgcaggaac tttcactgca tgataaagcc aaaaagaaaa
     7741 atattacgat catacccgac tgcggattgg ctccgggtat ggtttctgtt ttggtagcgg
     7801 atggcatcgg gcaaatggat gagactacgg atgtgcagat acgcgtcggt ggtcttccgc
     7861 tggaaccgcg tcctccgatg aattataaac tggttttttc ggtgcacggg ttgatcaacg
     7921 aatacgtcga accttgcgtg aaaatccaaa gcggagaagt tgttaccgtg gacccaatgg
     7981 tggatgtgga ggagctggaa tttcccgcgc cgttcggcaa actggaagcg tttaatactt
     8041 cgggcggcac ctcgacactg ccgcagacgt atttaggaaa agtgaaaaat ttggattaca
     8101 aaacgatacg atatcccggt cacgccgcac agttcaagtt gctgatggat ctcggactga
     8161 cggaatccaa accggtcaaa gtcggcaacg atacggtcgt accgcgcgat ctgctcgcac
     8221 aaaatctaac caaagtgctg acgatggaag gccgcgatgc cgtattggtg cgcgttaccg
     8281 tcaaaggaat aaaaggcgga aatcccaaag aaatccaata ccaaatcgtg gactatggcg
     8341 atgcgtccaa taatatcacc gccatgatgc gtctgacgtc ctatccgatt tctgtgatcg
     8401 cacaaatgat gaccgaaggc aaaatcacgg aaaaaggcgc cgttccgcag gaattagctg
     8461 ttccaacacg gtcttttatc gaagcgctca gagcgcgaaa tattgacatc cagattacag
     8521 aatcttaagc catcttaatt tgatggttat agcttttgta gtgaaataaa aaaatattcc
     8581 agataagtag ccgttatatt tttttttgct taagggatgc agtgcgccca atctgcgcat
     8641 gatcgctgtc gtattcttgt tttataccca ccgaatcggc cctgtttcaa actgatgcag
     8701 gtagcctcat gaagaaacat tcctaactat cgaatttatt tgaacttgtg acctgcatca
     8761 catcgttttg aagcgatgct ttgattttat tgggtttaat tatccagtca tattttggca
     8821 aggtatttgt cttatataaa aacaattaac gcgatgataa cctttcattc actaaaatga
     8881 ggtaaatcgg atatgagacg caccacttca gcagcacgca aaaccaagca gaatcccgtg
     8941 ttgatggatc tggagaatta tattttgaaa ttgggtttga attccgaacc cacctcggcc
     9001 aatgtcgttg ccaagcggct tgaactcaaa gagagcccca aaaatattca gatgatcaag
     9061 caggctttgg aaaaaatggt gcgtgaaaaa aaggtgtacc gctgcggaag ccgctatgcg
     9121 attgattatc agggcggatt caatcttatg aaaacatttt gcatcaaagt cgcagaagag
     9181 aattataaaa taaacaattt tgtatttgag ggtgttgctt acgcccgcgg ggcataattt
     9241 ttccgattcc tatcggttac cactagggag cggcgcatga cttcggttag gcgccgtttc
     9301 ttttttaagg tagcatgttt ttatttccgg ccttatcttt tcaaaaaaac cacggaagaa
     9361 gacgcattca tgcaagagct tatccgcgtc gaatccctct gtaagtcata cccggtaggg
     9421 agcaccttat gggggcgtgc gacctggtcg caagtcgtcg aagatgtttc ttttatcgtc
     9481 cgaaccggag aaacccttgg tgtggtcggc gaatcgggct ccggaaaaag tacgatcggg
     9541 cgatgccttc tcaaactgac cgatccgacg tcgggacgta ttttttttga cggcgaagaa
     9601 attaccgcat tggataataa agcgatgcgt ccttttcgcc gccgcatgca aatcgtattt
     9661 caggatccgt ttagttcact caatccccgc caaacggtgg atgccatgtt tcgtgagata
     9721 ttcaccgtgc acgcgacagc ttccggcacg gaggcgacag ataaaatcga agaattgctt
     9781 aaaaccgtcg gtcttgatcc ctcacagcgg ttttgttttc cgcacgaatt ttcaggcgga
     9841 cagcgccagc gtatcggtat agcacgggcc ttgtcggtcc ggccgcaatt catc
//
LOCUS       DAOQNQ010000102         9855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2499_length_9855_cov_4.805000, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000102 DAOQNQ010000000
VERSION     DAOQNQ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9855
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2499_length_9855_cov_4.805000"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PK754_07845"
     CDS             complement(<1..245)
                     /locus_tag="PK754_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09454.1"
                     /translation="MTTFSDILLNLRRQSKSKPATLPKNECTTYRGSVCTICPLIQSN
                     YNDELRIKNEAIREFWRMHRLDGKPESIIASPLSRRYR"
     gene            378..1076
                     /locus_tag="PK754_07850"
     CDS             378..1076
                     /locus_tag="PK754_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB09455.1"
                     /translation="MAFLPFRILVIEDDPDISRNINKLLEHAGYVCATCDEGSVGFEK
                     ACSGMWDAVILDIHLPVKDGLDIVRDIRRANINVPVLMVTGRNSVPDKVAGLKLGADD
                     YLTKPFHNDELLARLDALLRRFGRAQTVSPSEVYKHAGLFADFKSGEFKLENKPFKLT
                     AHEMKMLHYLVQHQGLIITRDELLTHVWKYGDTVTTRTVDVHVAAIRQKIEKIPSKPK
                     RLISIYGLGYKFIG"
     gene            complement(1097..2209)
                     /locus_tag="PK754_07855"
     CDS             complement(1097..2209)
                     /locus_tag="PK754_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09456.1"
                     /translation="MRKLGVILLLLQTRLIMAQATVSDAQILADVRRSRPDAATVKVV
                     GSSWSFYKDKGYLYNNRAEKTVDLTTTKNSDGVSFSMRGAAVYESSGPGQPYMYSRLF
                     VFESSVTMIGGPNYSVTDLQKIAYETARRDILRWFSNPQSIARVYEIKVPENHDLRMS
                     ALDHMSFRVQQTVLRVDNTNTVHKEVHEDVLTFRKNKDGWFVTSPLTYAKKKTSLGSM
                     KPGTRVLSSVPSLQDVGLDRLYGDSKDDFFKDGVDQGEKSPTAKDIFSRNKSQNDPSG
                     GITIPKKKDDGESVTDLPEDTQVRNDIMSRDADVAAVSFIGNWNTRKTKEGGGVNLAG
                     RMVVLKKKNQNSDKFVVVYNKDNGLWKLKAVEKSPQ"
     gene            complement(2623..3162)
                     /locus_tag="PK754_07860"
     CDS             complement(2623..3162)
                     /locus_tag="PK754_07860"
                     /inference="COORDINATES: protein motif:HMM:NF019320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HNB09457.1"
                     /translation="MHGIVFIELRKFVEVGFGRDGWMQILKKADIGEDLFMPSKTYPD
                     HQIVSIVLAACEITGKSAHEILDAFGKFIVPDLIGIYGSFIDPKWTMIDMIENVETTI
                     HRIVRMKNAGADPPELSCTRLGKNKIEIAYKSKRKMCAFAKGLVAGLANHYNVKATVT
                     ETLCMHNGDAECRMQVVTS"
     gene            complement(3537..6737)
                     /locus_tag="PK754_07865"
     CDS             complement(3537..6737)
                     /locus_tag="PK754_07865"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB09458.1"
                     /translation="MKLLRWHNLVFLIGMMHPIALCVYSQNERLYFDLVDLPKTMSPA
                     VRSIVQDHQGLIWVGTEKGLGRYDGYSWRVFHSDATDSFLISDANVWTVFEDRKGYLW
                     IGTINGGVNVLDRNRSRFHHYKHQPNNTASLSNNTVYSILEDNKGVMWIGTENGLNCI
                     YPESNKVMRLPDQTTDPSGYKGKIIHSLAMDSLYRIWIGSDSGLTVYDPQTCQFSVFQ
                     VQEHGRIYTPRRVRKLYADEDRQTLWIGTFGGGLYRWGIHTHEIKQYDVKSVENAYPS
                     NYVRNLVTDRDHEHLWIAIRGGGLGRLHKASGQIRYFTRQPNIPLSIPDNASLALYRD
                     DLGSIWVGFNYDGLAIMNYRKSWFQHIRRDATGTLVDHTVRSVCVAGDGTTLFGVQRA
                     VHRYDPHTDRLQSFVMPEHVVNGFNQVQAILYDKGQNYWIGTYGNGLNRLDAATGKFS
                     KYDAYPSGVQQRILSLTRFGDFLWIGTPSEIIRIHPATSSSRIYKNIRAVNSFAQTNT
                     QKLIAGSGTNGLFFYDAVADSFMPWLSSSMKYPSDYETRSLFADSQYIVWQGTSNGLN
                     KINHKTGEITRYTRNDGIPSTDVTGIIQSPDQRLWISTTSGILRLDPGQSQFRYYYKE
                     NGLLGNEFSLSAVAMSSEGRIFFGGIQGVTWFNASDVRDYDYKPTLLITKISRFYQDV
                     DGEIPYNSIEAQYNENVLTFEYSVLDYAFSAGIHYAYRLEGFNQTWIQTDERHATFTN
                     LDPGEYVFRVRGTRGDGTWIDSESKVFITIHPPYWLTWWFWSLVVFCIGMVTWMGYRM
                     RVRSLLRRKRELQTEVERQTKELYAKNIELQSLNDTKNEFLGMAAHDLRNPLGAVGNI
                     AELMLFEMNPENPETKQTFSDLNRIIQLTERMSYLIHELLDYSAIESGKVSLAVDTID
                     WTVLVHEVCEMNERAASRKKIVIKKEFSDGTIVLHGDRMRLMSVLDNLVSNAIKYSFA
                     GTEVLIRSEVANRKLRVTVYDHGQGLPEADVARAFKSFQKLSAKPTGGESSTGLGLAI
                     IKKIIDLHAGQVWLESELHKGSTFGFSLPLERPFSD"
     gene            complement(6744..8297)
                     /gene="guaA"
                     /locus_tag="PK754_07870"
     CDS             complement(6744..8297)
                     /gene="guaA"
                     /locus_tag="PK754_07870"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNB09459.1"
                     /translation="MSHGETILILDFGSQFTQLIARRVRELQVYCEIHPYNMTPDKIR
                     ALQPKGIILSGGPSSVYENGAPHPDRAIFDLGIPVLGVCYGMQVMAYFLGGAVDKAAH
                     REFGRAEIRITDHTDLFSGFPEKTTVWQSHGDRLEKIPDGFEIIAGTDNAPITAMRNK
                     ARRFWGVQFHPEVAHTPLGINLLKNFVRTICACTGQWTPSSFVKETTDAIRAKVGDGY
                     VICALSGGVDSSVVAMLLHQAIPDRFSAIFVDNGLLRKNERGQVEAAFRSFFNNNLIV
                     ADASELFLTRLKDVTDPEKKRKIIGATFIDVFETEVEKLRATTGHIDFLAQGTLYPDV
                     IESVSIKGPSATIKTHHNVGGLPEKMKLQLIEPLRELFKDEVRAVGRQLGLPDDLLMR
                     HPFPGPGLAVRVLGEVTKERCDLLREADAIFMEELKKSGEYSNVWQAFTVLLPVKSVG
                     VMGDERTYQNAVAIRAVTSQDGMTADWAHLPYDVLGRISNRIINEVRGINRVTYDISS
                     KPPATIEWE"
     gene            complement(8326..8748)
                     /locus_tag="PK754_07875"
     CDS             complement(8326..8748)
                     /locus_tag="PK754_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNB09460.1"
                     /translation="MPERTHRMAVTAYIVHDGKFLLLKRTNPPMIWGPPGGRLNPDEN
                     PNEGVLREVFEECGLTVKILHPVDIWYGDFGRGIYVSVDYLAISPSSEVSLSDEHSDY
                     IWSDMNALRNGQPSLGSAAPAFTIHDFQRAWELYQKLL"
     gene            complement(8741..9274)
                     /locus_tag="PK754_07880"
     CDS             complement(8741..9274)
                     /locus_tag="PK754_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNB09461.1"
                     /translation="MSLKDLPIGKKSPDEVHAIVEIPSGSRNKYEYDVELEIFKLDRT
                     LYSAVHYPTAYGFIPSTFYDDGDPLDILIVASQPLHVGVLVEARPVGVLRMRDDKGPD
                     DKILGVAIRDEQFRDIHRVEQLPHHLLVEIEHFFTTYKHLEGKDVRSFGWEPEAFAKS
                     AIMRGHQKYLERKSSHA"
     gene            complement(9264..9719)
                     /locus_tag="PK754_07885"
     CDS             complement(9264..9719)
                     /locus_tag="PK754_07885"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HNB09462.1"
                     /translation="MMNPHASDESSITVKQIETKPLSRPMRILFVIAGHIFVALGVIG
                     AILPLMPTTIFLILAGWCYARSSERFHCWLYTNKFFGTYLRNYKENRGTPLSVKIGSI
                     SVLWISIGISIWTTSMPWFVPWILIVIASAVTVHLARLKTLKKESSHES"
BASE COUNT         2758 a         2353 c         2046 g         2698 t
ORIGIN      
        1 cggtaccggc gcgaaagcgg agaagcgatg atagactccg gttttccgtc taacctgtgc
       61 atgcgccaaa attctctgat cgcctcgttt ttgattcgca attcgtcatt ataattagac
      121 tggatgagcg ggcatattgt acaaaccgat ccgcggtacg tcgtacattc atttttcggt
      181 aaagtggccg gcttggattt ggattgccta cgtagattta aaagaatatc agaaaatgtg
      241 gtcatggtac ttatagcatt ttgtttacaa tgaacgccta tagtaccgtt tctgaattaa
      301 caatgcaaca cacgaataaa ctcgggcaat tcgtgcggaa tgcttgaatc tgcggcataa
      361 tatttgtatt ttttttcatg gcatttttac cttttcgtat tttagtcatt gaagatgatc
      421 cggacatttc ccgtaatatc aacaaactgc ttgagcatgc cggttatgtc tgcgcgacgt
      481 gcgatgaagg aagcgttggt tttgaaaaag cctgtagcgg catgtgggat gcggtgattc
      541 tcgatatcca ccttcccgtc aaagacggat tggatattgt acgtgatatc cgccgggcta
      601 atatcaatgt accggtattg atggtcacag gaagaaactc cgtaccggat aaagtagccg
      661 gactcaagct tggggcggat gactatctga ccaagccttt tcataatgac gaacttttgg
      721 cgcgactgga tgccttgcta cgacgattcg gtcgagcaca aacagtatct ccctctgaag
      781 tttacaagca cgccggatta tttgccgatt ttaaaagcgg ggaatttaaa ttagaaaata
      841 aaccgtttaa actgactgcc catgaaatga agatgctgca ctatttagta cagcatcaag
      901 ggttgatcat aacacgggat gaattattaa cacatgtctg gaaatacggc gataccgtca
      961 caacacgaac ggtcgatgta cacgtagctg ctatccgaca aaaaatcgaa aaaataccat
     1021 ccaaaccaaa acggctgatt tctatttacg gcttaggtta caaatttatc ggttaattgc
     1081 tggcgcctat ttgagtttat tgcgggcttt tttcgaccgc tttcaatttc cacaaaccgt
     1141 tatctttgtt gtaaacaact acaaatttat ctgagttttg gttttttttc ttaaggacga
     1201 ccatccgacc tgccaaattc acaccgccac cttctttggt tttacgtgta ttccagttac
     1261 cgataaagga taccgccgca acatccgcat ctcggctcat gatatcattg cgaacttggg
     1321 tgtcctccgg aagatctgtt acggattcgc catcatcttt tttttttgga atcgtgatac
     1381 cgccggatgg atcattctgc gacttattgc gtgaaaagat gtctttggcc gtaggcgatt
     1441 tttcaccctg atccactcca tctttaaaaa aatcatcttt ggaatcgcca tataaacgat
     1501 ccagacctac atcctgcaaa cttggtacgg acgataaaac acgagtaccg ggtttcatgc
     1561 tgcccagcga tgtcttcttt ttagcatagg tcagagggga tgtaacaaac catccgtctt
     1621 tgttttttct aaatgtgaga acgtcttcgt ggacctcttt atgcacagta tttgtattat
     1681 ccacacgcaa caccgtttgt tgaacccgaa aactcatgtg atccagtgcg gacatacgga
     1741 gatcgtgatt ttcaggtact ttgatttcat aaacgcgagc aatactttgc ggattgctaa
     1801 accacctcaa aatatcgcga cgcgccgttt cgtatgctat tttttgcaga tccgttaccg
     1861 agtaatttgg accaccgatc atggttaccg aactttcaaa cacaaaaaga cgactataca
     1921 tatacggttg tcccgggccc gatgattcgt aaacagcggc tcctcgcatc gaaaacgaaa
     1981 caccgtccga atttttggtc gttgtcaaat ctacggtttt ttcggcacgg ttattataaa
     2041 gatacccttt atctttataa aatgaccacg atgaccctac gactttcacc gtagccgcat
     2101 ccggtcgcga acggcgcaca tccgccaata tctgtgcatc gctgaccgtg gcttgtgcca
     2161 tgatgagtcg tgtttgcaaa agtagaagaa taacccccaa tttacgcatg tttttactcc
     2221 tttgaatttt ggaatttatt gattcacaaa caaaacatca tagtcattca cattcaagca
     2281 gacgttgcac cgcgttatct gttttcatgt ggcattactt tttttgatac aaagacttac
     2341 ttcgtcattt attcatccaa gcataaacaa ttcacggcag taccgtgtta gtgcggcgta
     2401 aaaagaatgt aaaaaaagag ttaagctgca ggcaaagaaa cagccgtatt ttagatcggc
     2461 ataacacgta cgatagatgc aaaatttata ttgcatgccg tgctgcaatc aaaaccattt
     2521 gcgacgaatg attacgcagc gaagttgatt agactattta tatcgtactt aaggcaaagc
     2581 aaagaccggg cttctaatca acggctcgga aacagcacac acttacgagg taaccacttg
     2641 cattcggcat tcggcatctc cgttgtgcat acaaagcgtc tccgttactg tagctttaac
     2701 gttataatga ttcgctaatc ccgctaccaa tcctttggca aatgcgcaca ttttacgctt
     2761 ggatttataa gcgatttcaa ttttgttttt tcctaaccgc gtacaactca actccggcgg
     2821 atcggcgcct gcatttttca tacgcacgat acggtgaatg gtggtttcta cattttcgat
     2881 catatctatc atcgtccact tggggtcaat aaaagagcca taaataccga tcaagtccgg
     2941 aactatgaac ttcccaaaag catcaagtat ttcatgggct gattttccag ttatttcgca
     3001 cgcggccaaa acgatagata caatctgatg atccgggtac gttttagacg gcataaacaa
     3061 atcttcgccg atatcggctt tctttaagat ctgcatccaa ccgtcacggc cgaagcccac
     3121 ttctacaaat tttctcagct ctataaatac aattccgtgc ataggatttg acctttaatt
     3181 atgaattatc attaataatg aattattatg ttgggctttg aaaaaatttg ttcattaatt
     3241 tttttatttg cgttaaggca atccattgca aaacaaaaaa agccgactta aatagtctta
     3301 aaaaacgcaa aaaaataccg atgcatctta gggttgtttt aaagcgcctc atggatccga
     3361 ttgttatgtt ggagcatgtt caagtcggct atttatcgta atgcaagatt ggacgtataa
     3421 tccatccgta actgcgccat cataatcatt tttgcgaatg gcagtgttag actactgtaa
     3481 aaagaatgtt aaaaaagtgt taaggcaaac caggtggggc aatttactat ccaaacttaa
     3541 tcggagaacg ggcgctccag cgggagagaa aaaccaaaag tagaaccttt atgcaattcg
     3601 gactcaagcc aaacttgtcc ggcgtggagg tcaataattt tcttaataat agccaggccc
     3661 agtccggtac tactttcacc gccggtcggt ttggctgata gtttttgaaa agatttgaat
     3721 gcgcgagcca catccgcttc cggcaatcct tggccatgat catataccgt gacacgaagc
     3781 tttcgatttg ccacttcgga acgaatcaat acttcagtac ccgcaaaact gtatttgatc
     3841 gcattggaca ccagattgtc caacaccgac atgagtcgca tgcgatcgcc gtgaagtacg
     3901 atcgtcccat cgctaaattc ttttttgata acaatcttct ttcgcgatgc cgcgcgctca
     3961 ttcatttcgc atacttcgtg tacaagcacg gtccaatcaa tcgtatctac ggctaacgaa
     4021 actttacccg attcgatcgc tgaataatca agcaattcgt gtatcagata actcatgcgt
     4081 tccgtaagtt gaataatccg attaagatcg ctgaaggtct gtttggtttc ggggttttcc
     4141 ggattcattt caaataacat taactcggca atattcccta cagcgccaag cgggttgcga
     4201 agatcatgtg cggccatacc caaaaattca tttttggtat cgttcaagga ttgcagttcg
     4261 atattttttg catacaattc cttcgtttgt ctttcgactt cggtttgcaa ctcacgctta
     4321 cgacgcaaca aactgcgcac gcgcatacga tatcccatcc acgtaaccat tcctatacaa
     4381 aacacaacaa ggctccaaaa ccaccatgta agccagtacg gaggatgaat agtaataaaa
     4441 actttacttt cggaatcaat ccaggttcca tcacctcgcg ttccacgaac gcggaaaacg
     4501 tattcgcccg gatccaaatt ggtaaacgtc gcatgccttt catcggtttg aatccatgtc
     4561 tgattaaaac cctctaaacg ataggcataa tgaatacctg ccgaaaacgc ataatccagt
     4621 accgagtatt caaatgtcaa aacgttttca ttgtattgtg cttcgatgct gttataaggt
     4681 atctcgccgt caacatcttg atagaaacgc gatattttag taatgagtag agtcggcttg
     4741 tagtcataat cacgaacatc ggaggcatta aaccaggtca caccttgaat acctccaaaa
     4801 aaaatacgcc cttccgaact catagcaaca gccgacaaac tgaactcatt acccagcaaa
     4861 ccgttttctt tatagtaata acgaaattgc gattgaccgg gatcaagacg caatatgccg
     4921 cttgtcgtgc tgatccagag cctttgatca ggactttgaa taataccggt cacatcggta
     4981 ctcggaattc catcgttcct cgtataacgt gttatttctc cggttttatg gtttatttta
     5041 tttaaaccat tggacgtacc ttgccatacg atatactgtg aatcagcaaa aagcgaacgc
     5101 gtttcgtaat ccgacggata tttcattgac gaagaaagcc atggcataaa agaatcggcg
     5161 acggcatcat aaaaaaataa tccgtttgtc cccgaaccag caatcaattt ttgagtattg
     5221 gtctgagcaa atgaattgac cgctctaata ttcttataaa ttcttgatga actggtagcg
     5281 ggatgaattc gtataatttc cgacggcgtc ccgatccata aaaaatcccc gaaccgagtc
     5341 agtgataaaa ttcgctgttg cactccggaa ggataggcgt cgtatttgct aaattttcct
     5401 gtggccgcat ccaagcgatt gagcccgttg ccataggtac cgatccaata gttttgacct
     5461 ttatcgtaca atatggcctg aacttgattg aaaccgttca ccacgtgttc cggcatcacg
     5521 aatgattgta atcgatctgt atggggatca taacgatgta cggcacgctg tactccaaaa
     5581 agggtcgtac cgtcgcccgc tacacaaacc gaacgaaccg tgtgatccac caatgtaccg
     5641 gtcgcatcgc ggcgaatatg ttgaaaccaa gacttgcggt aattcataat cgcaagcccg
     5701 tcgtaattga aacccaccca gatactgccg agatcatcac ggtacaatgc aagcgatgca
     5761 ttatcaggaa tcgaaagcgg gatgtttggc tgtcgtgtaa aataccggat ttgaccggaa
     5821 gctttgtgca atcgtccgag gcctccgccg cgtattgcta tccataaatg ttcgtgatcg
     5881 cgatccgtta cgagattgcg cacataattt gaaggataag cgttttcgac ggatttgaca
     5941 tcatattgct taatctcgtg cgtatgaata ccccagcgat aaagtccgcc tccgaaagtg
     6001 ccaatccata acgtttgacg atcttcatcc gcatacaatt tgcgcacacg gcgaggagta
     6061 taaatgcgac catgctcttg cacctgaaat actgaaaatt gacaggtttg gggatcgtat
     6121 acggtaagtc cggaatcgct gcctatccaa atgcgataca acgaatccat tgcaagggaa
     6181 tggatgattt ttcctttgta accggacgga tcggtggttt gatccggcag gcgcataacc
     6241 ttgttacttt caggataaat acaatttaat ccgttttccg taccgatcca catcacgcct
     6301 ttattgtctt ctaagataga gtagaccgta ttattactaa gcgaagccgt attgttgggt
     6361 tgatgtttat agtgatgaaa ccgagaacga ttccgatcca atacattgac gccgccattg
     6421 atcgtaccaa tccacagata acccttacga tcttcaaaca ccgtccaaac attcgcatca
     6481 ctgattaaaa aactatccgt tgcgtccgaa tgaaaaactc gccacgaata accgtcgtag
     6541 cggccaagcc ctttttccgt tccaacccaa atcagtcctt gatgatcctg cacgatactg
     6601 cgaacggcag gactcatcgt cttaggcaga tcaaccaaat cgaaatacaa gcgttcattc
     6661 tgtgaataaa cacacaatgc tatcgggtgc atcattccga tcaaaaaaac aaggttatgc
     6721 caacggagca atttcataca tgattactcc cactcaatcg tagccggagg cttggaactg
     6781 atatcgtacg ttacgcgatt gataccgcga acctcattaa taatccggtt ggaaatgcga
     6841 cccaatacgt cgtacggcag atgcgcccaa tcggcggtca tgccatcctg acttgtgacg
     6901 gcacgtatag cgacggcatt ttggtaagta cgttcatccc ccatcacacc gaccgatttg
     6961 acaggaagca aaacggtaaa ggcttgccaa acattggaat actcgcctga ttttttcaat
     7021 tcttccataa agatcgcatc ggcttcgcgc aataaatcac agcgttcttt cgttacctcg
     7081 ccgagcacac gtacggccag accgggaccc gggaacgggt gacgcataag cagatcatcg
     7141 ggcaaaccta actggcgacc gacggcgcgc acttcatctt tgaaaagttc gcgcaggggt
     7201 tcgattaatt gcagtttcat tttttccggt aatccgccga cattgtgatg tgttttgatc
     7261 gtagccgagg gtcctttgat ggatacgctt tcgatcacat ccggatagag cgtaccttgc
     7321 gctaaaaagt caatgtgacc cgttgtggca cgcaactttt caacttccgt ttcaaacaca
     7381 tcgataaacg ttgcgccgat gatttttctt tttttctccg gatcggtcac atctttgagt
     7441 cgcgtcaaaa aaagttcgga cgcatccgcc acaatcagat tattattaaa aaaagaacga
     7501 aacgccgctt cgacttgccc gcgttcgttt ttccgaagca gtccgttatc tacaaaaata
     7561 gccgaaaaac gatccggtat ggcctgatgc aatagcatcg ctactacgga tgaatccacg
     7621 ccaccggaga gcgcacaaat cacataaccg tcaccgacct tagcacgaat agcatccgtc
     7681 gtttctttga caaatgaaga aggtgtccat tgacccgtac acgcgcaaat ggtgcgaacg
     7741 aaatttttga gaagattgat acccagcggt gtatgcgcca cttccgggtg aaactgaacg
     7801 ccccaaaaac gacgcgcttt atttcgcatt gccgtgatcg gcgcattgtc cgtaccggcg
     7861 atgatttcaa atccatcggg aattttttcc aaacgatctc cgtggctctg ccataccgtc
     7921 gttttttcag gaaagccgct gaacaaatcc gtgtgatcgg taatgcgaat ttcagcccga
     7981 ccgaactccc gatgtgccgc tttatctaca gcaccgccga gaaaataagc cataacctgc
     8041 atcccgtaac atacccccaa tacgggaatt cccaaatcga agatggcacg atccggatgc
     8101 ggcgctccgt tttcatacac actgcttggt ccgcccgaca ggataattcc tttaggttgc
     8161 agcgcgcgaa ttttgtccgg cgtcatattg tacggatgta tttcgcaata cacctgcagt
     8221 tcgcgaacac gccgtgcgat caattgggta aattgtgatc cgaaatccag tattaaaata
     8281 gtttcaccgt gactcacgtt gcgattcctt tattacaatt ataatttata atagtttttg
     8341 atacagttcc catgcccgtt gaaaatcatg gatcgtaaag gccggtgccg cactgcccaa
     8401 ggagggttgc ccgttacgca acgcattcat atccgaccat atataatcac tatgttcgtc
     8461 tgaaagtgat acttcgctcg acggcgaaat ggctaaataa tccaccgaca catagatccc
     8521 gcgcccgaaa tcaccgtacc aaatatccac cggatgaagt atttttaccg tgagaccgca
     8581 ctcttcaaat acttcccgca acactccttc attcggattt tcatccggat tgagtcttcc
     8641 gcccggcggt ccccaaatca tcggaggatt ggttcttttc aacaaaagaa atttaccatc
     8701 gtgaacgatg tatgccgtca ccgccatacg atgcgtacgt tcaggcatgc gacgatttcc
     8761 gttccagata tttctgatga ccacgcataa tagccgattt ggcaaacgcc tccggttccc
     8821 aaccaaaact gcgtacgtct ttaccttcga gatgtttata cgtggtaaaa aaatgttcta
     8881 tttcgacaag caaatgatgc ggcagttgct ccacccggtg aatatcgcga aactgttcgt
     8941 cacgtatcgc cacacccaga attttatcat ccggaccttt atcatcccgc atgcgtaaaa
     9001 caccgaccgg acgcgcttcg accaatactc cgacatgaag cggttgcgat gccacgatca
     9061 aaatatcaag cggatcaccg tcatcataaa atgtactcgg aataaaacca taggccgtag
     9121 gataatgcac ggccgaatac aacgtgcggt cgagtttaaa tatttcaagt tctacgtcgt
     9181 attcgtattt attacgactg ccgctcggaa tttcgacgat cgcgtgcact tcgtcgggtg
     9241 atttttttcc gatcgggaga tctttaagac tcatgcgatg attctttctt taatgttttt
     9301 aatcgcgcaa gatggacggt aacggcggat gcaataacga tcaatatcca cggcacaaac
     9361 cacggcatgg acgtcgtcca aatgctgatg ccgatagaaa tccacaatac ggagatcgaa
     9421 ccgattttga cagacaatgg tgtgccgcga ttttctttat aattgcgaag ataagttccg
     9481 aagaatttat tggtgtataa ccagcaatga aatcgctcgg agcttcgcgc ataacaccat
     9541 ccggcgagaa tgagaaaaat cgtcgtcggc atcaacggta aaatagcacc gatcacaccc
     9601 aaggctacga aaatgtggcc tgctatgaca aacaaaatac gcatcggacg actgagtggc
     9661 tttgtttcga tctgtttgac ggttattgat gattcgtcag aagcgtgcgg attcatcata
     9721 atcccttcat cattttattg gcggcaacaa ggatcggatc ccataccggt gcaaacggcg
     9781 gagtatacat catttgcata tccgcgattt ggcgcagagt cattttttgt tgaatagcta
     9841 acgccagtac attgg
//
LOCUS       DAOQNQ010000103         9836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2507_length_9836_cov_5.698088, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000103 DAOQNQ010000000
VERSION     DAOQNQ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9836
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2507_length_9836_cov_5.698088"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PK754_07890"
     CDS             <1..267
                     /locus_tag="PK754_07890"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB09463.1"
                     /translation="RITDDMTLLFKNFKPSSVRRIEMIKSVRATSNNHAKDSYRFHSR
                     TGIQGLYLAGDWIADELPSTIESAVLSGIRCAEAVLQDEKNKRS"
     gene            275..736
                     /locus_tag="PK754_07895"
     CDS             275..736
                     /locus_tag="PK754_07895"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNB09464.1"
                     /translation="MLKKNAIISELTWRDSVGQEHTLAQYSRHKAVVFFFYPMDFTRV
                     CTGQACYFRDVYGEIAAQGGVVFGVSLDDDATHEKFRTHHRLPYALIYDYDRTLSKKF
                     DTLWLWGLWRNKRVTYIIRPSDGKIVDVIHAERDAQVHADRVLAYLRASSL"
     gene            complement(731..2836)
                     /gene="glgP"
                     /locus_tag="PK754_07900"
     CDS             complement(731..2836)
                     /gene="glgP"
                     /locus_tag="PK754_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNB09465.1"
                     /translation="MDQRLNDLTDLAYNLWWSWNQNAQSVFRELSPQIWEESNHNAVA
                     VLHGTSHQELRARLNDSHFREKVDRVITQFRSYMRHENTWGSHHAQTFRDKPVAYFSP
                     EFALHECLPIYSGGLGVLSGDHIKSASDLDIPFVGVGLFYRQGYFQQHIAIDGTQQEL
                     YPLNDTTHLPLSLLSDRTGKPVVTVVEIGHSLVSLYCWQVKVGRAQLYLLDSNHPDNE
                     EHYRELTSRTYGGDIMTRICQEIVMGIGGVRLLRSIGIRPSVYHMNEGHSAFLTLELL
                     RERMLEGQPMDTARAWVREHCVFTTHTPVPAGHDRFSRDLMTFALANYCAILGLTIDQ
                     VMGLGRVHPDDEKETFCMTVLALKMSRASNAVSALHGNVSRDMWKELFPDRPVEQVPI
                     SHITNGIHILSWMNKRTRNFWRKFLGENWEQHIKQPDFWQRVADPSFISDEEIWALRY
                     DLRRMLIEFMRVRLKEQQRRAGGDGVRVFSNFFSPDILTIGFARRFATYKRAPMIVQN
                     LERAIQFFNDQDQPIQVVFSGKAHPKDDSGKAFIKRIVELSKHPQLFGKLAFIENYDL
                     YVARFLTSGCDVWLNNPRRPLEASGTSGQKIGIHGGLNLSILDGWWREGYDGTNGFAI
                     GKDESLGNEAEQDRLDEENLYLTLKNEVIPAFYNRDANGIPRVWIQKIRRAMQTLIPV
                     FNTDRMVAEYFETMYRRNS"
     gene            3017..4267
                     /gene="pepT"
                     /locus_tag="PK754_07905"
     CDS             3017..4267
                     /gene="pepT"
                     /locus_tag="PK754_07905"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HNB09466.1"
                     /translation="MFDPKYTFTCVDRFLKYVKYDTQSDEESTSFPSTEKQKNLSRDL
                     AAELKAMGLADAHMDEYGYVMATLPGNTSKNVDPIAFIAHVDTSPAVTGAGVNPVINK
                     NYQGGDIRLKNDSNQIIEVKNNPDLEKMIGFDIITTDGTTLLGADNKAGVAEIMDAVN
                     YFLSHPEVKHGPIKVCFTPDEEVGRGTEKIDLKKLGAKYAYTVDGSSRGEIETETFSA
                     DGMTIRFHGKNIHPGYAKGKMVNAIKVASHFIENLPKVGLSPETTEKREGFVHPTSFT
                     ANEELATIRFIIRDFVTAKLKEKEDFLKDLAEKTAAQFPGARVECEVKEQYRNMKEVL
                     DHHPQVGAYAVEAMKRLGIEPIQTPIRGGTDGSRLSFMGLPCPNIFAGEHSFHSKLEW
                     VAVQDMEAAVKTIIEIAQIWEEKA"
     gene            4457..5293
                     /gene="ygiD"
                     /locus_tag="PK754_07910"
     CDS             4457..5293
                     /gene="ygiD"
                     /locus_tag="PK754_07910"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HNB09467.1"
                     /translation="MRLREFSSITKSLPNTESMPILFLGHGSPMNAIEENEFVRGWRR
                     IGELLPRPKAILCISAHWETRGTYVTAMDKPKTIHDFGGFPKSLYEVEYPAPGSPDLA
                     LETQSIITKTKVGLDHGQWGLDHGCWSVVKHLYPNADIPIIQMSLDHYQHAEYHYELA
                     KEFHVLRQKGILIVGSGNMVHNLRMVAWDKLNDTAYGYDWAIEANETMKQKILTNDHS
                     ALIHYDKMGRAFDMAIPTPDHFLPLLYILALQKPDEKISFFNDKPVAGSLTMTSVLIH
                     NE"
     gene            5390..6250
                     /locus_tag="PK754_07915"
     CDS             5390..6250
                     /locus_tag="PK754_07915"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNB09468.1"
                     /translation="METQEQINYKRIAEAIEYIRVNFKEQPDLDDVAKKVHVSPFHFQ
                     RLFTAWAGVSPKKFLQFTTIDYAKSILKDRKISLMDAAMESGLSGTSRLHDLFVNIEG
                     MTPGEYKNGGENLEINYSFSESPFGDILSASTSKGLCYMMFSDDPKSALSQLVSHFPN
                     ATYHHKKDSFHKNALNIFNVVAPENHSINLHLKGSEFQLKVWEALLKIPFGKLTTYNT
                     IAKEIRKPKASRAVGTAIGSNPVAYLIPCHRVIQSSGNFGGYMWGPTRKTAIIGWEAA
                     KISAQHEIVL"
     gene            6528..6821
                     /locus_tag="PK754_07920"
     CDS             6528..6821
                     /locus_tag="PK754_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09469.1"
                     /translation="MACSEGANIGTSENDKALFGSDEKISGVGYIKYVTVEGGYFELI
                     TSDNKIFDVHDLPDEYKKDGIVVKFTGHINKELVCVHAAAPILTLKIIERLEH"
     gene            6842..7231
                     /locus_tag="PK754_07925"
     CDS             6842..7231
                     /locus_tag="PK754_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09470.1"
                     /translation="MHHKAIMLLCVITVSLFGCDSKKESGIDGTQWQTVSVMRLKEER
                     VHFENGTYHYWIKLHPSGCYEYAYYPYSETEKMKGKICTPIKMPDDICLGSFEVSNDT
                     LYVRFGEVVTAWTQVEFDDDQLLICGE"
     gene            7502..8146
                     /locus_tag="PK754_07930"
     CDS             7502..8146
                     /locus_tag="PK754_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09471.1"
                     /translation="MYDSLRYNHQTQYWSVRFERSNQSYELQYRYESPDQEGNPTSKS
                     DVMYLEFNYKNNHPIGSGAKAYDVEGTAKYTIKGIKNYSDPGKVGELYYSGSTISYFQ
                     YQFSLDTVVKFTYRDIVQNVVLSEQSCVPNSGYFECTMTQDAVPGTFTIEPEIERVFY
                     QSKLKDVPNQTFKDYEFRGKTLFTKEGIHILTDGEDFEISMPCDSIQNLIDTGH"
     gene            8218..8718
                     /locus_tag="PK754_07935"
     CDS             8218..8718
                     /locus_tag="PK754_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09472.1"
                     /translation="MQITRAGILSILFIAVVSCTEKDSGLFYETSFESINDLKAWKNV
                     NESQRINKAAPGAGEYSMAVGAGCIQPAAVFEKQVVASGFYRLRMWARMEENSASNAL
                     FSLTASDAENLSRIDLSVESGEWKLYESEEFEMRHGENFKLEVFCGGITFSQVNIDGL
                     QILQVR"
     gene            8848..>9836
                     /locus_tag="PK754_07940"
     CDS             8848..>9836
                     /locus_tag="PK754_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNB09473.1"
                     /translation="MTFRWTMRTRIGVLFSVITMILIIVLSRFSYVAVREVYLDQLSR
                     QVRMSAKAIATDMDMGNIKFVQPYSENSVATQFYKRYLKKKKVGHELASAFIFDAALR
                     MVMTSDDESDYIHPDPTLLLSQSEIERLAPGETSTTLPFLGNDNEWYMWGFCRIDDEY
                     WAGVRAKADQLMYIENLSFVFWFISGTALVFTLFGSFWLAKTITKPIDQMVRFSRSLG
                     RGEWDTEAPKHVKGELASLATAMEQMRTDIQGRHKEKEELLAQIAHEIRNPLGGIELM
                     AGLVYEDMPPESQQSAYIRQIRKEVSGLKNLVHQYLEFGRPAQAVGQLIDIRS"
BASE COUNT         2951 a         1977 c         2245 g         2663 t
ORIGIN      
        1 agaataacag atgacatgac actcctgttt aaaaatttta aacctagttc ggttcgtcgt
       61 atcgaaatga tcaagtcggt gcgcgccacc tcgaacaatc atgcgaaaga ttcgtatcgt
      121 tttcatagtc gcactggaat tcaagggttg tatttagccg gagactggat cgccgatgaa
      181 ttaccttcga cgatcgaaag cgcggtactt agcggtatcc gatgcgcgga ggccgtgttg
      241 caagacgaaa aaaacaaaag gagttaatct ctctatgctt aaaaaaaatg ctattatttc
      301 tgaattaact tggcgggatt cggtcggtca ggaacatacg cttgcgcaat atagccggca
      361 caaagctgta gttttctttt tttatccgat ggattttaca cgcgtttgta ccgggcaagc
      421 gtgttacttt cgcgatgtat atggcgaaat cgcggcacaa ggcggtgtgg tttttggcgt
      481 cagtttggat gacgacgcga cccatgaaaa atttcgcaca catcatcggt taccgtacgc
      541 actcatttac gactatgatc ggacattaag caaaaagttt gatacgctgt ggttgtgggg
      601 actctggcgc aataaaagag tcacgtatat cattcgtccg tcggatggaa aaatagtgga
      661 cgtgattcat gccgaaaggg atgcgcaagt tcacgcggat cgcgtactcg catatcttcg
      721 tgcgtcgtcg ttatgaattg cgacgataca tggtttcaaa atattcggcg accatacgat
      781 ccgtattaaa caccggaatc aaggtttgca tggcgcggcg aattttttgg atccacacgc
      841 ggggaatacc gttggcatcg cgattgtaga atgcgggtat cacttcattt ttgagtgtca
      901 gataaagatt ttcttcgtct aagcgatcct gttccgcttc attgcccagc gactcatcct
      961 taccgatagc aaatccgtta gtcccgtcat aaccttcacg ccaccatccg tcaagaatgg
     1021 ataaattgag tccgccgtgt atgccgattt tttgcccact ggtaccgctg gcttcaagag
     1081 gacgacgggg attattaagc caaacgtcgc agccggatgt aagaaaccgg gcaacataaa
     1141 gatcgtaatt ttcgataaag gccagtttgc caaaaagttg gggatgtttg ctcaattcga
     1201 caatgcgttt gatgaacgct tttccgctgt cgtctttggg gtgggcttta cctgaaaaca
     1261 caacctgaat cggttgatcc tggtcattga aaaattgaat cgcccgttcg aggttttgaa
     1321 caatcatggg tgcgcgttta taggtagcaa aacggcgcgc gaaaccgatg gttaatatat
     1381 ctggcgaaaa aaagtttgag aacacgcgca caccgtctcc gcccgcacga cgttgttgtt
     1441 ctttgaggcg tacacgcata aactcgatga gcatgcgccg gagatcataa cgcagcgccc
     1501 agatttcttc atcggaaata aacgaaggat cggctacgcg ttgccagaaa tccggttgtt
     1561 tgatatgttg ttcccagttt tcaccgagaa atttgcgcca aaaattacgg gtgcgcttat
     1621 tcatccagct taagatgtgg ataccgttgg taatatgtga aatcggcact tgctcaacag
     1681 gacgatccgg aaaaagttct ttccacatat cgcggctgac attaccgtgc aacgcgctga
     1741 cggcattgga tgcgcgagac attttgagtg ccagtacggt catacaaaat gtttcttttt
     1801 catcgtccgg atgcacgcgt cccaggccca taacctgatc aatcgttaat ccaagaatcg
     1861 cgcaatagtt ggctaatgca aacgtcatca ggtcgcggct aaaacgatca tggcccgcag
     1921 gaaccggcgt atgcgtggta aaaacacaat gttcacgaac ccatgcccgc gccgtatcca
     1981 tcggctggcc ttcaagcata cgttcacgga gcagttccag tgtgagaaat gcgctatggc
     2041 cttcattcat gtgatatacg gacggacgaa ttccgataga acggagcaat cgaacaccgc
     2101 caatgcccat cacgatttcc tggcaaatgc gcgtcataat atcaccgccg taggtgcgtg
     2161 atgttagttc gcgataatgt tcttcgttat ccggatgatt gctatcgagc aaatacagtt
     2221 gagcgcgacc gaccttgact tgccagcagt ataagctgac cagactgtgt ccgatttcaa
     2281 ctacagttac tacaggtttt cctgtacggt cggaaagaag tgacaaaggc aagtgcgtcg
     2341 tatcattgag cgggtagagt tcctgctgtg taccgtcaat ggcgatatgt tgctgaaaat
     2401 aaccctgacg ataaaacaaa ccgacaccga caaatggaat atccaaatcg ctggccgact
     2461 tgatatgatc gccggagagt acgccgagcc cgcctgaata aatcggtaaa cattcatgca
     2521 atgcaaattc aggactgaaa taggcgaccg gcttatcacg aaacgtttga gcgtgatgcg
     2581 atccccaggt gttttcgtgc cgcatatagc tgcgaaactg cgtaataaca cgatccacct
     2641 tttcacgaaa atgtgaatcg ttgagacgtg cacgcaattc ttggtggctc gttccgtgca
     2701 aaaccgcaac tgcgttatga ttgctttcct cccagatttg cggggataat tcgcggaaaa
     2761 ccgattgcgc gttttgattc catgaccacc agaggttgta tgccaaatcc gtaagatcgt
     2821 ttaatcgttg atccataatt ccttgcagtt tcgttacgtg ggggcacagt ataaaatttc
     2881 ccgcgatgaa catcaagcgg taaaacataa tcgcatagaa tgataggtac aatcgtaagc
     2941 cgtatcttga tgttttacaa aaaaaaaggt accattgttt atcaatacta tttacaactc
     3001 cattaagggc aaaactatgt ttgatcccaa gtatacattt acctgcgtgg atcgtttttt
     3061 gaaatacgtc aaatacgata cccagtcgga tgaagagtcc acttcttttc caagtaccga
     3121 aaaacaaaaa aatttatccc gcgatcttgc cgctgaattg aaagcgatgg gattggccga
     3181 tgcgcacatg gacgagtatg gttatgtgat ggcgacattg cccggaaata cctccaaaaa
     3241 cgtggatcct attgcattta ttgcacacgt ggatacatcg ccggcagtga ccggtgccgg
     3301 tgtcaatccg gtgatcaata aaaattacca aggcggcgat attcgcctca aaaacgattc
     3361 caatcaaatt atcgaggtaa aaaacaaccc tgatcttgaa aaaatgatcg gctttgacat
     3421 cattaccacg gatggtacga cgctactcgg cgcggataat aaagccggtg tggcggaaat
     3481 catggatgcg gttaattatt ttttgtctca tccggaagtc aagcacggcc cgatcaaagt
     3541 atgttttaca ccggatgaag aagtaggccg cggtacggaa aaaattgatt taaaaaaatt
     3601 aggcgccaaa tatgcctata cggtggacgg ctccagtcgc ggtgaaatcg agaccgagac
     3661 gtttagcgcc gatggtatga cgatccgatt tcatggaaaa aatatccacc ccggatatgc
     3721 taaaggaaaa atggtcaatg ccatcaaagt ggcatctcat tttatcgaaa atttaccaaa
     3781 ggtcggtctc tctccagaaa caacggagaa gcgcgagggg tttgttcatc cgacatcgtt
     3841 tacagccaat gaagaattgg cgaccatacg ttttatcatt cgtgattttg tgacggcaaa
     3901 actcaaagaa aaagaagatt ttctcaaaga tcttgctgaa aaaacggccg cacaatttcc
     3961 cggcgcccgc gtagagtgcg aagtcaaaga gcaataccgc aatatgaaag aagtgcttga
     4021 tcatcatccg caagtcggag cgtatgccgt cgaagcgatg aaacgcctcg ggatcgaacc
     4081 tatccaaaca ccgattcgcg gcggcacgga cggatcaagg ctttcgttta tgggtttacc
     4141 ttgccccaat atttttgccg gtgaacatag tttccattcc aagctggagt gggtagccgt
     4201 gcaagacatg gaagccgccg taaaaaccat catcgaaatc gcgcagattt gggaagaaaa
     4261 agcgtgaccg agtgaaactt gcttttagat gttcaatcaa aacctccgca aaaaacggag
     4321 gtttttgatt agggttttta gtctcgaatg accaaaaaat catgtgtaaa attgataagt
     4381 acttcttata gttttgttaa tgaatcgaaa acaatttatt catacgatgg cattaatacc
     4441 attagccgga gttaatatgc gactgcgcga attttcatcc atcacaaaat ctttgcctaa
     4501 tactgaaagc atgccgattt tgtttctcgg tcatggcagc cccatgaatg ctatagagga
     4561 aaatgagttt gtccgaggat ggcgtcgcat aggagaactc ttaccaaggc ctaaggcaat
     4621 tttgtgcatt tcagctcatt gggagacgcg gggaacctat gtgacggcca tggacaaacc
     4681 caaaacgatt cacgacttcg gtggatttcc aaaatcgttg tatgaagtag aatatcccgc
     4741 acccggtagt ccggatttag ccttagaaac acaatccatc ataaccaaaa ccaaagtcgg
     4801 cttggatcat ggacaatggg gattggatca tggatgctgg agtgtggtca aacatttata
     4861 tccgaatgct gacataccga tcatacaaat gagccttgac cattatcaac acgctgaata
     4921 ccactacgaa ttagctaaag aattccacgt tttaaggcag aaaggcattt tgatcgtggg
     4981 gagcggaaat atggtacata atctgcgtat ggttgcttgg gataaactca atgacacggc
     5041 ctatggttat gattgggcca tcgaagcgaa tgaaaccatg aaacaaaaaa tccttacaaa
     5101 cgatcacagt gctctcatac actacgataa aatgggacgt gcttttgata tggccatacc
     5161 gacgccagat cattttttgc ctctactgta tattctggcg ttacagaaac cggatgaaaa
     5221 aataagtttt tttaatgaca aaccggttgc cgggtctttg acgatgacgt cggttttgat
     5281 tcacaatgaa taatgatttc agaatgactt actttgatcc aaaaatagca agaatcgggt
     5341 tttgctgaga gttttgagat agtatcttta tcctaaaaaa ggagaggata tggaaacgca
     5401 agagcagata aattacaaaa gaatagcgga agcgatcgaa tatatcaggg ttaatttcaa
     5461 agaacagcct gatttggatg acgtggctaa aaaagtacat gtaagcccgt ttcattttca
     5521 acgtttgttt acggcatggg ccggggtaag tccaaaaaag tttttacagt ttacaacgat
     5581 agattatgca aaaagtattt tgaaagaccg taaaattagt ttgatggatg ccgctatgga
     5641 aagcggatta tcgggaacaa gccgcttgca tgaccttttt gttaatatcg aaggtatgac
     5701 gcccggagaa tataaaaatg gaggagaaaa tctcgaaata aactatagtt tttcagaaag
     5761 tccttttggt gacatacttt cagcttctac gtccaaaggc ctgtgttaca tgatgttctc
     5821 cgatgacccc aaatcagcgt tatcgcaatt agtgtcacat tttcctaatg ccacatatca
     5881 tcacaagaag gattcgtttc ataaaaatgc gctgaatatt tttaatgtag tagcgcctga
     5941 aaatcattcg atcaatttac atttaaaagg aagcgagttt cagctcaaag tatgggaagc
     6001 gttgttaaaa attcccttcg gaaagctgac tacatataat accattgcta aagaaattcg
     6061 taagccgaaa gcttcgcgtg ccgtgggtac agcgattggc agtaatcccg ttgcatattt
     6121 gataccatgc cacagagtga tccagtcttc aggaaacttt ggcggctata tgtggggtcc
     6181 aacccgtaaa acggcaatca tcggatggga agccgctaag atatctgcgc aacatgaaat
     6241 tgttttgtaa tttgtaaatc aaatatccca acctgactct tttcaaatca tatcaaatcc
     6301 cgatttctta tcaaatatcc gctatagtgt cggttttctg ataaccgaga actgaaaatc
     6361 gagtaaaaac aaaactcact gtattgtttt ttattggttt aactgacttc atttttgttc
     6421 ctcattggca ctaagtttgt agctttatta ttcaacattt cgtcatacta atctatttca
     6481 cttcgatgct aatcaagtta aacatactaa caaccttatt tctcttaatg gcgtgttcgg
     6541 aaggcgcgaa tattggcaca tccgaaaacg ataaagcgtt gttcggttcc gacgaaaaga
     6601 tatcgggcgt cggctacata aagtatgtga ccgttgaagg tggttatttc gaattgataa
     6661 ccagtgataa caaaattttt gatgtgcatg atttaccgga tgaatataaa aaagacggta
     6721 tcgtggtaaa atttacaggc catataaata aagaattagt atgtgttcat gctgctgcac
     6781 ccatactaac tttgaaaatt atagaacgct tagaacatta acttatatga aggtacgagt
     6841 tatgcaccat aaagcgataa tgctattatg tgtaattaca gtttcattat tcggttgtga
     6901 ttctaaaaaa gaatcaggaa tagacggcac acaatggcaa actgtaagcg tcatgcggtt
     6961 aaaagaggag cgagtacatt ttgaaaatgg aacatatcat tattggataa aactgcatcc
     7021 ttcgggttgt tatgaatatg catactatcc gtactctgag acggaaaaaa tgaagggaaa
     7081 aatatgcaca cctataaaaa tgccggatga tatctgtctc ggttcctttg aagtgagtaa
     7141 tgatacgctg tatgttcgtt ttggtgaagt ggttacagct tggacacaag tagaatttga
     7201 tgatgaccaa cttttaatct gtggtgaata agaaagtgat gtgttcgttt tgtgtagagc
     7261 aaaaaaaata ttcggtcttt tgttcatttc gttgaatttg atttcatgtt atgacgcatc
     7321 agaaaaaaaa atgagccgcg aagaattaaa cgatattgtc gagactcaag gccgtgcaat
     7381 gctaagcaat agcttggtac aattaaataa cgcttcttac ctcgctcaaa gggagatcgc
     7441 tgatttacct ggcttgttca tactatctcc ttggggtttt cggtttgaag gagaatcatc
     7501 catgtatgat tctctgagat ataaccacca aactcaatat tggtccgttc gttttgagcg
     7561 atcaaatcaa agttatgaat tgcaataccg atatgaatca cccgatcagg aaggcaatcc
     7621 tacatcaaag agcgatgtca tgtatttaga gtttaattat aaaaataatc accccatcgg
     7681 ctccggtgca aaggcctatg acgtggaggg tacagccaaa tatacgataa aaggtatcaa
     7741 aaattattct gatccgggca aggttggcga attgtattat agcggatcca cgataagtta
     7801 ctttcaatat caattttctc tggatacggt tgttaaattt acgtatcgcg atattgttca
     7861 aaacgtcgtg ctgagcgaac agagctgcgt gccaaacagc ggatactttg aatgtaccat
     7921 gacgcaagat gcggtgcctg ggacttttac catagagcct gaaatagaaa gagtatttta
     7981 tcagtcaaag cttaaagacg ttcctaatca gacgttcaaa gattatgaat tcagaggcaa
     8041 aacattgttt accaaagaag gcatccacat tttgacggat ggagaagatt ttgaaatatc
     8101 aatgccttgc gattcgattc aaaatttaat tgatacaggc cattaataac gtgatctaaa
     8161 tttttaaaaa gtatgtacag ttatctcaaa caattactta gaaaggagag tgatttcatg
     8221 caaataacgc gagcaggtat cctctcaata ctatttattg cagtcgtatc ttgcactgaa
     8281 aaagattcag gactctttta tgaaacgtca tttgaatcaa taaatgacct caaagcatgg
     8341 aaaaatgtca acgaaagtca acgtatcaat aaagcggcgc ctggtgcggg cgaatacagt
     8401 atggccgtgg gggccggatg tattcaacct gcagcagttt ttgaaaaaca ggttgtcgct
     8461 tcgggtttct atcgtttacg aatgtgggca cgtatggaag aaaacagtgc cagcaacgct
     8521 ttattttcac ttacggcgtc cgatgctgaa aatctaagtc ggattgattt gtccgtcgaa
     8581 agcggtgagt ggaaactcta tgagtctgaa gaatttgaaa tgcgacacgg tgaaaatttt
     8641 aaattggaag tattttgtgg tggtataact tttagtcaag taaatataga cggcctgcaa
     8701 attttgcagg tgcggtaatg aagagcactc gcccgggaat tttataaacc ctctccctcc
     8761 tcataacccg ggcgggtgta tttttaaaac aagtgagcgg ctgttgcaat taatatgcaa
     8821 aaggagcaac tttaagtaaa aaagcttatg acattccgat ggacgatgcg tactcggatc
     8881 ggggttcttt tcagcgtgat cacgatgatt ctcatcatcg tgctttcgcg ttttagttac
     8941 gtagccgtcc gagaagttta tctggatcaa ttaagccgtc aggtgcgtat gtccgccaaa
     9001 gcgatcgcaa cggatatgga tatgggaaat ataaaatttg tccaacccta ttcggaaaac
     9061 tccgtagcca cacagtttta caagcgatat ctaaagaaga aaaaagtagg acacgaactg
     9121 gcgagtgcat ttatttttga tgcggcattg cgaatggtga tgacgagtga tgatgaatcc
     9181 gattatatcc atccggatcc tacattactg ctcagtcaat ctgaaattga acgattagct
     9241 ccaggcgaaa cttcgacgac gttacccttt ttgggtaatg acaacgaatg gtatatgtgg
     9301 ggattttgta gaatagatga tgaatattgg gccggtgtac gtgccaaggc cgatcaattg
     9361 atgtacattg aaaacttatc ctttgtgttt tggttcataa gcggaacggc attagtattt
     9421 actttatttg gaagtttttg gctggctaaa acgattacta aaccgattga ccaaatggtg
     9481 cggttcagcc gttcgttggg acgtggggaa tgggataccg aagcgcctaa acatgtcaaa
     9541 ggcgaattgg cctcgttggc cacggctatg gaacaaatgc gtacggatat acaaggtcgt
     9601 cacaaagaaa aagaggaact gttggcgcaa atcgcgcatg aaataagaaa tcctttgggc
     9661 ggtatcgaac tgatggccgg cttggtttac gaggatatgc cgccagagtc acaacaaagc
     9721 gcgtacatcc ggcaaataag aaaggaagtt tcaggactta aaaatcttgt tcaccaatat
     9781 ttagaattcg gacgtccggc acaagctgta ggccaactta tagacatcag gtcgtt
//
LOCUS       DAOQNQ010000104         9830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2508_length_9830_cov_6.057698, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000104 DAOQNQ010000000
VERSION     DAOQNQ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9830
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2508_length_9830_cov_6.057698"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PK754_07945"
     CDS             <1..292
                     /locus_tag="PK754_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB09474.1"
                     /translation="THLFTVPNRLSFFDLGGVRLMLGIAEPGEYALSRPILYYKTSHL
                     DEEFARLKSVGVVVVHQPQLVAPMPDHDLWMAFFKDPEDNTLGIMEERSKNK"
     gene            370..1098
                     /locus_tag="PK754_07950"
     CDS             370..1098
                     /locus_tag="PK754_07950"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HNB09475.1"
                     /translation="METMTEPKPLGALDRIIGVITEPEKTFTDIAVRPSWGLPMMLAL
                     LAMIAVGILMKDVIQKESAEKTRISIMENKNIPDGDKQRIIDQSIEMMKKFWVVGFAV
                     GMVVIVIAYFISALILKLGGSAVLKSTATFTQVLTIFSWSGIVDLLGALIKLPIMYIN
                     QSMRVDTGLGILIGEELGHTTTYLIMSKFDIFTIWMLIVMSIGIAVVFKAPKSKSMIL
                     VFGLWLVWVLAQWGMALMGMRVGG"
     gene            1334..2653
                     /locus_tag="PK754_07955"
     CDS             1334..2653
                     /locus_tag="PK754_07955"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNB09476.1"
                     /translation="MKHLLTIVWIALAANLTAQQKQALTIEEALTIAMKNNVQVISAQ
                     NDYNSASANVLPKTVGKILPTVDASMRASRTMMNTLSGTSPLTNRDTTTGSASNSYSY
                     SLTANYVLFDGFRNWTEASQARLDESAARYNFERTRQSVTLDVYEAYINVLKNQQLLR
                     INEENLKRSEEQLKRLEERNKLGAQILSDVYKQRVQVGSDKLSVSKSKNNLKTSKASL
                     ASLIGIDVNSDLELKELIIASSLDVNQMNFDEAYGQALQNRKDYLASESRVQSARRTV
                     SIARSGFYPTVNAFASYQWGDKNLKFANYSNNDNTSIGLSVSIPIFSGFETNSRVIQQ
                     DQSLQTARSNAESTKRKVALDVKIALLNMQTAYDNMKLSEENVKAAKEDLRLATEKYN
                     LGAGTILDQITANASYAAAEANYTQSAYDFVYAKQQYLLAVGTLDVQ"
     gene            2689..3984
                     /locus_tag="PK754_07960"
     CDS             2689..3984
                     /locus_tag="PK754_07960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB09477.1"
                     /translation="MSKRNKILIGVGLTLVLTVVIVLNIRGKKEDAVEVQTEKVKRGD
                     VIKTVSASGKIQPVTDIKISSNVSAKILQLTVKEGDKVKRGQVLVSLDRTFYDAQLDQ
                     AKAELLRAKSQVATAQANYEKAQQDFSRVKDLTAKQLASASELEVAQTTLSTTRASVE
                     SAESQVQAAEASAQQAADNLSKTTIYAPIDGTISKLNKKVGEIALGSQFSQDVIMIVA
                     NLNEMEARVNVDENDVVAVEIGDTAVIEVDALTEQKLRGRVTEIANSATVKATGTSEE
                     KTEFEVKIALDDTPLMLRPGMSTTSDITTDVRGNTLKVPIQCVTVREPERLKRKEGVE
                     KEGAAMASDTNTQFVPNKDGVVEIVFVIENGMAVARPVKTGIQSESHIEILDGIKEND
                     EVVVGNFRAISKILENGSKVKVDNEKKPNKDDNKEARDN"
     gene            4015..4740
                     /locus_tag="PK754_07965"
     CDS             4015..4740
                     /locus_tag="PK754_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB09478.1"
                     /translation="MLIDIRNITKRYQIGTEEVHALRGVSLQIKKNDYIALMGPSGSG
                     KSTMMNVIGCLDTPTSGEYLFEGQDVAQMNDDQLAEIRNRKIGFVFQNFNLIPRSDVF
                     HNVELPMIYAGHAPAERKRLTIEAIEKVGLGARMKHKPNELSGGQRQRVAIARALVNN
                     PSIILADEPTGNLDSKTGEEIMQVFEQLHKAGNTIILVTHEEDIASHAHRVVRLKDGV
                     VETDEAITGRKVFKKVLVEAEAQ"
     gene            4756..5994
                     /locus_tag="PK754_07970"
     CDS             4756..5994
                     /locus_tag="PK754_07970"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB09479.1"
                     /translation="MKRFIYETLEAFRIAMTAIRANKARGFLTTLGIVIGITAVSTTM
                     TVMNGMKTSFQSQLSVLGNDVLYVTRQPWIQMDDWWKYRNRPQINLEEAERLEKSLGD
                     KVVAINPTVGTMRPVKYESKTLDDIFIKGTTDKELLTSSTTPEFGRYIGAIDVQNSKY
                     VCVIGAEVKKQLFENVDPIGRRIRVGAYNFRIVGVLEKQGKLLGNFGGPNFDNQVAIP
                     ITTFMKSFGRSRGLSLAVKVQSVSKLDETQDEIIGAMRKIRKLSPSRDDNFSVNRQDS
                     FLKIYDSIMGVVGLVGILITGISLFVGGIGVMNIMFVSVTERTKEIGIRKAIGAKRRT
                     ILIQFLFEAAMICLIGCAIALVLSYLLSVVIDTFFAASLSLGIVIVAIVMAIMVGIIS
                     GLLPALKASKLDPIDALRYE"
     gene            6027..7277
                     /locus_tag="PK754_07975"
     CDS             6027..7277
                     /locus_tag="PK754_07975"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB09480.1"
                     /translation="MKIDMRENLKMALNAVFANKLRSFLTLVGVVAGVASIIAVMTGI
                     AVIQGKMEADMSQLGSGTFQVQKWPAIGGPGQNWRKIRQRKPLTIEQANAIRERIRDA
                     ELVGAELWEQGSVLQHEEEKTNPNVSLAGGTPEFQINNNQLVGVGRFISEEDVKVGRN
                     VIVIGTTIAKKLFPFKDPIDQIVKINGHKYTVIGIYEEMGASASGSGDNSACIPISAF
                     VDQFGLKDNQGMPRSVNITVKARAGANVENVMEEVRGLLRQVRGVKPLDEDDFDMYSN
                     DTMIRAFGEMTVIIKLVAIVMGGIALIVAGIGIMNIMLVSVTERTKEIGIRKAIGAKR
                     KDIMQQFLIEAIVLCEIGGAIGIMVGMAIGNIVSILMSVPVKLPLDWAIIGLVFCSGI
                     GIAFGIWPASKASKLDPIESLRFE"
     gene            7398..8180
                     /locus_tag="PK754_07980"
     CDS             7398..8180
                     /locus_tag="PK754_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09481.1"
                     /translation="MKLIKFFLLMLSVWILISGCASRVTVQSQRVTQNILINGHDDDW
                     EGQMQIIEKTGVAIGIRNDDDYLYLALSPLNRGMAMQWMTAGLTVWLDSTGGKNKRFG
                     IHFPIGLSDLGIPPMIMMGMPGERDTKRDNPLEKMSDRLLEEVEIIGPGKYDKQRIGT
                     KESTTLQVRLTHNPNGMFYELRIPLQNDPRNVCAINTRPGKNLGIGLETGKMERRRGM
                     PPMGEGGMGGPRGGGRPGGGRPPEMERAEPLSVWISTTLKGF"
     gene            8271..8786
                     /locus_tag="PK754_07985"
     CDS             8271..8786
                     /locus_tag="PK754_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB09482.1"
                     /translation="MFDETFQKGENTVLREAGVHFRKAYELQMAGRLQDAIDEYQCSL
                     ALHPTAEALTFMGWAYSHLGDYAVAISKCEAAITVDPDFGNPYNDIGAYLISLQRLEE
                     AIPWLEKAKHARRYEARHYPFFNLGRIFEMQGRWFEAIAEYRGALHLYPNYQSAKDAV
                     TRLQILIDRRN"
     gene            8808..>9830
                     /locus_tag="PK754_07990"
     CDS             8808..>9830
                     /locus_tag="PK754_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09483.1"
                     /translation="MTRFVILLSLWVNGYAYAQTKALDVVYPREGVLIGSNDSMFVFG
                     CVKVAHQSLRINNMSTPTHEDGAFIQFLPITNVQKDSVFQMRFEIKTKDCTIVRYHRV
                     RIPLLPRPMDSDIPQIDSLSLKPSRVYRLQSGDDIPIACRATPHASVEFTIIDTTGKI
                     IDGPYPMAEYETDRMDDFPNAVYGEDRDTRPEPISGMYSATYRLCEGISYQRSRVAFR
                     VEKKKKSVTSVARGFIDLWPKHERLVAELNGPYHQPRTMPGQSFYYFLQGGLRILIDG
                     SIGSWRRLRFAENHSAWVSEDELTYAAPGTPVPASTIPVIRVRRDERTSVIKLTMSEK
                     LPYRVEQ"
BASE COUNT         2755 a         2084 c         2449 g         2542 t
ORIGIN      
        1 gacgcatctg tttactgttc cgaaccggtt atcttttttt gatcttggcg gtgttcgcct
       61 gatgttggga attgctgagc ccggtgaata cgctctgtcc cgacctattc tatattataa
      121 aacatctcac cttgatgagg aatttgcacg tctaaagtcc gtgggcgtgg ttgttgttca
      181 tcagcctcaa ttggtagcgc cgatgccgga tcatgatctt tggatggctt ttttcaagga
      241 tccggaagac aataccttag ggattatgga agaaaggtca aaaaataaat gaaacctgta
      301 agaaaaagta atttgcggca acgtatccga tagtaaataa aaaccatccc tttgttatca
      361 ggagatacta tggagacaat gacagaacct aaaccgttag gtgctttgga ccggataatc
      421 ggcgtgatca ccgagccgga aaagacattt accgacatag ctgtacggcc ctcatggggt
      481 ttgcccatga tgcttgcttt gctggccatg atcgcagttg gtatattgat gaaggatgtg
      541 atccaaaaag aaagcgccga aaaaacgcgc atttccatta tggaaaataa aaatatacct
      601 gacggcgaca aacaacgcat tattgatcag tctatcgaga tgatgaaaaa attttgggtc
      661 gtaggtttcg ctgtgggtat ggtcgttatc gttattgcat attttatctc ggcgttgata
      721 ctcaagttgg gcgggagcgc tgtgcttaag tcaacggcta cttttacgca agtgctgact
      781 attttctcct ggtcggggat cgtggatctt ttgggcgctc tgatcaagct gccgatcatg
      841 tatatcaatc aaagcatgcg cgtggatacc ggactaggca tcctcatcgg agaagaactc
      901 ggtcatacga cgacctactt gatcatgagt aaatttgata tctttaccat atggatgttg
      961 atcgtgatgt ctataggtat agctgtagtg ttcaaagcgc ctaaatcaaa atcgatgatt
     1021 ttggtttttg gtttgtggtt ggtctgggta ttggcacagt ggggtatggc gctgatgggt
     1081 atgcgtgtcg gaggttaatt ttttataaat tctatgatct aatgtgtata gccgttttct
     1141 tatggtaaag aagccataat aaaacggctt ttgtatgtgc aacatttttt gtctgaaaca
     1201 gtctaatcgg tataagaaat accgttaaac gaaaaaaagg ataatttttc gggaaaaagt
     1261 gaaacatttt gataaattcg ccgaatactc gattgtttgt atgaattacc gaattttgag
     1321 gagaatagga gacatgaaac atttattgac cattgtttgg atcgccttag ccgccaatct
     1381 tacggcacag caaaaacaag cgctcacgat tgaagaagcg ttgaccatcg cgatgaaaaa
     1441 caacgtacag gtcatttccg cacaaaacga ctacaattcc gcatccgcta acgtattgcc
     1501 taaaactgta gggaaaattt taccgacggt tgatgcgtca atgcgcgcga gtcgcacaat
     1561 gatgaataca ctttcaggta caagccccct gacgaatcgt gataccacga ccggcagtgc
     1621 ttctaatagc tatagttact cgttaactgc caattacgtt ttgtttgatg gttttcgtaa
     1681 ttggacagaa gcatcacaag cacgtctgga cgaatcggcg gcacgataca attttgaacg
     1741 tacgcgtcaa agcgtgacgt tggatgtgta tgaagcgtat atcaatgtcc ttaaaaacca
     1801 gcaattgctt cgcatcaatg aagaaaatct caagcgtagc gaagaacaac tcaagcgtct
     1861 tgaagaacgc aataaattag gcgcgcagat tttgagcgat gtgtacaaac aacgtgtgca
     1921 ggtcggctcg gataagctat ctgtcagcaa atccaaaaat aacctcaaga cttccaaagc
     1981 ttcattggct agtctgatcg gtattgatgt caattccgat ctcgagttaa aagagctaat
     2041 tattgcctca agtcttgacg tgaatcagat gaatttcgat gaagcgtatg gacaagcgct
     2101 tcaaaatcgt aaggattatt tagcctccga aagtcgtgtc caaagtgcgc gtcgcaccgt
     2161 aagtattgca cgaagcggtt tttatcctac ggtcaatgct tttgcgtcgt atcaatgggg
     2221 tgataagaat ttaaaatttg caaattacag taataacgat aacacctcaa taggtttaag
     2281 tgtttccatt cccattttta gtggatttga aaccaatagc cgcgtgattc aacaagatca
     2341 atcgttgcag acagcgcgca gtaatgccga gagcaccaaa cgtaaagttg ccttggacgt
     2401 gaaaatcgct ctgctcaata tgcagaccgc ctacgataac atgaagttat ccgaggaaaa
     2461 cgtcaaagcg gccaaagaag atcttcgttt ggctacagaa aaatacaatc tcggggcggg
     2521 cacgattttg gatcagatta ccgccaacgc tagttatgca gccgccgaag ccaactatac
     2581 ccagtctgct tatgattttg tgtatgccaa acaacagtac ttgctggccg ttggcacact
     2641 ggacgttcaa taagtaattt tgagaaaccc tttttaggag atagagctat gtctaaacgt
     2701 aacaaaattt tgatcggcgt cggtttaacg ctggtgctta cggtagtgat cgttctcaac
     2761 attcgcggta aaaaagaaga tgcggtcgaa gtgcagactg aaaaagtcaa acgcggtgat
     2821 gtgatcaaaa ccgtcagtgc ctccggtaaa atccagccgg tcacggatat caagatcagc
     2881 tccaatgtta gtgctaaaat attacaactt accgtaaaag aaggcgataa agtgaaacgc
     2941 ggccaagtgc tcgtgtcatt agatcggacc ttttacgatg cgcaattaga tcaggccaaa
     3001 gccgagcttt tgagggccaa gtcgcaagtg gcaacagccc aagccaatta tgaaaaagcg
     3061 cagcaggatt tttcacgcgt caaagatcta accgccaaac aattagcgtc ggcttcagaa
     3121 ttggaagtgg cacaaacaac actttcgact acccgcgctt cggtcgaatc ggccgagagc
     3181 caggtacagg cagccgaagc cagcgcgcaa caagcagcgg ataatctcag caaaacgacg
     3241 atttatgcac ccatcgatgg tacgatcagc aaactcaata aaaaagtcgg tgaaattgca
     3301 ctcggctcgc aattttcgca agatgtgatc atgatcgttg ccaatctcaa tgaaatggaa
     3361 gcacgcgtca atgtagatga aaatgatgtt gtggccgtcg aaatcggcga tacggccgtg
     3421 atcgaagtgg atgccttgac cgaacaaaaa ctgcgcggac gtgtgacgga aatcgcgaac
     3481 tccgcaaccg taaaagcgac cggtacatcg gaagaaaaaa ccgaatttga agtgaagatc
     3541 gcgttggacg atacgcctct gatgctgcgc cccggtatgt ctacgacatc ggatattacg
     3601 actgatgtac gcggtaatac gttgaaagta ccgatccaat gcgtcacggt acgcgaaccc
     3661 gagcgcctca aacgtaaaga aggcgtcgaa aaagaaggtg cggctatggc cagcgatacc
     3721 aatacgcagt tcgtgcccaa taaagacggt gtggtagaaa tcgtatttgt catcgaaaac
     3781 ggtatggccg tagcgcgtcc ggttaagacg ggcattcaga gcgaatcgca tatcgaaatt
     3841 cttgacggta tcaaagaaaa cgatgaagtc gtcgtcggaa acttccgcgc gatcagtaag
     3901 attttggaga acggttccaa agtcaaagtg gataacgaaa agaagccgaa taaagacgat
     3961 aacaaagaag cacgcgataa ttaatataca tcggacgctt ttgaggaaaa caatatgttg
     4021 attgatatac gtaatattac caagcgttac caaatcggaa ccgaagaagt gcatgcactg
     4081 cgtggtgtgt cgctgcaaat caaaaaaaac gattatatcg cgctcatggg accttcgggt
     4141 tccggtaaat ccaccatgat gaatgttatc ggttgtttgg atacgccgac cagcggtgag
     4201 tatcttttcg aagggcaaga tgtcgcgcag atgaatgatg atcagctggc ggagattcgt
     4261 aatcgcaaaa tcggatttgt attccaaaat tttaatctca taccgcgttc ggatgtgttt
     4321 cacaatgtag aattgccgat gatctatgcg ggccatgcgc cggcggaacg caagcgtttg
     4381 acgatcgaag ccatcgaaaa agtagggctc ggtgcgcgta tgaaacataa acccaacgaa
     4441 ctttccggtg gtcaacgcca acgcgttgct atcgcacgtg cattggtcaa taatccttcg
     4501 atcatacttg cggacgaacc gacgggtaat ttggattcca aaaccggcga agaaatcatg
     4561 caagtgtttg agcagcttca caaagccggc aatacgatca ttctggtcac ccacgaagag
     4621 gatatcgcat cccatgcgca tcgcgtcgtc cgtcttaaag acggtgttgt cgaaaccgat
     4681 gaagcgatca cgggccgcaa ggttttcaaa aaagtacttg tggaagcgga agcccaataa
     4741 ccttcgagaa ttattatgaa acgatttata tacgaaacac tcgaggcttt ccgtatcgcc
     4801 atgacggcta tacgtgccaa taaagcccgc ggctttttga ccacgctcgg catagtgatc
     4861 ggtattacgg ccgtttcgac gacgatgacc gtgatgaacg gaatgaaaac atcgtttcaa
     4921 tcgcaattat ccgttttagg aaatgacgtg ctgtatgtta cgcgtcagcc gtggatacag
     4981 atggatgact ggtggaaata tcgcaatcgt ccgcaaatca atctcgaaga agccgaacgc
     5041 ttggaaaaat cacttggcga caaagtcgtg gcgattaatc cgacggtggg tacaatgcgt
     5101 cccgtcaaat atgaatccaa gacgctcgat gatattttta tcaaggggac aacggataaa
     5161 gaattactta cttcgtccac tacacctgaa ttcggtcgtt atatcggcgc gattgatgta
     5221 caaaattcca agtacgtatg cgtgatcggt gccgaagtga aaaagcaact ctttgagaat
     5281 gtagatccca tcggacgtcg catacgcgtc ggtgcataca atttccgtat tgtcggcgta
     5341 ttggaaaaac aaggaaaact cctgggtaat tttggcggcc ctaattttga taaccaagtg
     5401 gccataccga ttacgacgtt tatgaaatcg ttcggtcgct cgcgtggttt gagtctcgct
     5461 gtcaaagtgc agtccgtcag taaattagat gaaacgcagg atgaaatcat cggtgcgatg
     5521 cgcaaaattc gtaagctatc accgtcgcgc gatgataatt tttccgtcaa tcgtcaggat
     5581 agttttttga aaatctatga cagcatcatg ggcgttgtgg gtttggtcgg tattttgatc
     5641 acgggtattt cgctgtttgt cggaggtatc ggcgtgatga atattatgtt tgtatcggtg
     5701 accgaacgaa ccaaagaaat cggcattcgc aaagccattg gtgccaagcg acgtacgata
     5761 ttgatccagt tcttatttga ggccgccatg atttgtttga tcggctgtgc gattgctctt
     5821 gtattgtcgt acctgctcag tgtcgtgatc gatacttttt ttgccgcgtc attgtccctc
     5881 ggtatcgtga ttgtggctat tgtgatggcg atcatggtag gtattatctc aggacttctg
     5941 ccggcgctca aagcttcgaa actggatccg attgatgcgt tgcgttacga ataaaaaagg
     6001 aatgaatttc aggaacagag gtcgctatga aaattgatat gcgtgaaaat ctgaagatgg
     6061 cgcttaatgc ggtatttgcc aataaacttc gttcctttct gacgctcgtc ggtgttgtag
     6121 ccggcgtagc atctatcatt gcagttatga cgggcatcgc tgtgatacaa ggtaaaatgg
     6181 aagccgatat gagccaattg ggctcgggca cttttcaggt ccagaaatgg ccggcgatcg
     6241 gcggacccgg gcaaaactgg cgaaaaatca ggcaacgcaa gccgcttacc atcgaacagg
     6301 ccaatgctat acgcgagcgt attcgtgatg cagagctcgt cggggctgag ctttgggaac
     6361 aaggttccgt gttgcagcac gaagaggaaa aaactaatcc taatgtatct ttagccggtg
     6421 gtacaccgga atttcagatc aataataacc agttggtggg tgtcggtcgt tttatttccg
     6481 aagaagatgt caaagtcggt cgtaacgtga ttgtgatcgg tacgacgatc gcaaaaaaac
     6541 tttttccttt caaagatcca atagatcaga ttgtcaaaat caacggtcat aagtatacgg
     6601 tgatcggtat atatgaagaa atgggcgctt cggcttccgg gtccggcgat aacagcgcat
     6661 gtatacctat ttcagctttt gtggatcagt ttggtctgaa agacaatcaa ggtatgccgc
     6721 gttcggtgaa tattaccgtc aaagcgcgtg ccggtgcgaa tgtcgaaaac gtgatggaag
     6781 aagtacgtgg attgcttcgg caagtacgcg gcgtaaaacc cctcgacgaa gatgattttg
     6841 atatgtattc caatgatacg atgatccgcg cattcggcga aatgacggtg atcatcaaac
     6901 tggtagccat cgtaatgggc ggtattgctc tgatcgttgc gggtatcggt attatgaata
     6961 tcatgctcgt ttcggtgacg gagcgtacca aagaaattgg tattcgtaaa gctatcggcg
     7021 ccaagcgtaa agacatcatg cagcagtttt tgatcgaagc gatcgtactg tgcgaaatcg
     7081 gcggagcgat aggtatcatg gtcggtatgg ctatcggaaa tatcgtaagc attttgatga
     7141 gcgtaccggt aaaactgccg cttgattggg ccattatagg actcgtattc tgtagcggaa
     7201 tcggtatcgc gttcggcatt tggccggcct ccaaagcatc caaactggat ccgatcgaat
     7261 cgttgcggtt tgaataattg aatttttgaa aataatcaga gaaaaaggct gtgttgatca
     7321 cagccttttt ttgttttaac ttttttggag ctatcgcgtc taaaaagcaa aattatctta
     7381 taaaatagat tgagaccatg aaattgatca aattttttct tttgatgttg tcagtatgga
     7441 ttctcatatc cggctgcgcc tcgcgtgtta ctgttcaaag tcaacgcgta actcagaata
     7501 tattaatcaa tggtcatgac gatgattggg aaggtcagat gcagatcatc gaaaaaaccg
     7561 gtgttgcgat aggtattcgc aacgacgatg actatcttta tttggcattg tcaccgctta
     7621 accgcggtat ggccatgcaa tggatgaccg caggacttac ggtatggttg gattcgacgg
     7681 gaggaaaaaa taagcgcttt ggaattcatt ttccgatagg cttgtccgat cttggtattc
     7741 ctccgatgat catgatgggc atgcccggtg agcgcgatac aaagagggat aatcctttgg
     7801 aaaagatgtc cgatagattg ctcgaagagg tggagatcat cggtcccggc aaatatgata
     7861 aacaacgcat cggcaccaaa gaatcaacta cactgcaagt gcgcctgacg cacaatccga
     7921 atggcatgtt ttacgaattg cgaataccgt tgcaaaatga tccgcgcaat gtctgtgcga
     7981 ttaatacaag gccgggaaag aacctcggca tcggattaga aacaggcaag atggaacgta
     8041 ggcgcggtat gccgccgatg ggagaaggtg ggatgggcgg gccgcgtggt ggtggccgac
     8101 cgggaggcgg tcgacctccg gaaatggaac gagcagaacc gttgtccgtg tggatttcaa
     8161 cgactctaaa aggattctaa ataatttaat atcttcattt ctatccttga atgtcgcacc
     8221 gcgccgtggt agattaaccg gcaaatcaaa aggttcaatt tttaaacggc gtgttcgacg
     8281 aaacatttca aaaaggcgaa aatacggttc tacgcgaagc cggcgttcat tttcgcaagg
     8341 catacgaatt gcaaatggcc gggcgcttac aggacgcgat agatgaatat cagtgttcgc
     8401 ttgcgttgca tccgacggcc gaagcgttga catttatggg ctgggcgtat agtcacctcg
     8461 gtgattatgc ggtagcgatc agtaaatgcg aagccgccat cactgtagat cccgactttg
     8521 gtaatcctta taacgatatc ggcgcctacc tgatttcgct ccaacgattg gaagaggcca
     8581 tcccctggct tgaaaaagcc aaacatgcgc gtcgttacga agcccgacat tatccttttt
     8641 ttaacctcgg gcggattttt gaaatgcagg gacgctggtt tgaagcgatc gccgaatacc
     8701 gtggcgcgtt gcatctctat cccaattatc aatctgccaa agacgccgtg acgcgcctgc
     8761 aaattctcat tgaccgaaga aattaatcca aaacaacgga tattcacgtg acacgatttg
     8821 tgatactgct atctctatgg gtaaacgggt acgcgtatgc gcagaccaaa gcgcttgacg
     8881 tggtgtatcc ccgcgaaggc gtactgatcg gatcaaacga ttccatgttt gtttttgggt
     8941 gcgtcaaagt cgcacatcag tcgcttcgga ttaataacat gtcaacgccg acacatgaag
     9001 acggtgcgtt tattcaattt ttgccgataa ccaatgtcca aaaggactcg gtttttcaaa
     9061 tgcgtttcga aatcaaaaca aaagattgta cgatcgtacg ctatcatcgt gtgcgtattc
     9121 ctcttttacc acgacccatg gattctgaca ttccacaaat tgattcgtta tcacttaagc
     9181 cgtcgcgtgt gtaccgtctt cagtcgggtg atgatattcc gatcgcctgc cgtgccaccc
     9241 cgcatgcgtc ggtggaattt actattatag atacgaccgg aaaaataata gacggccctt
     9301 atccgatggc tgaatacgaa acggatagga tggatgattt tcccaatgcg gtatacggtg
     9361 aagatcgtga tacacgcccg gaaccgatat cgggaatgta ttccgcgacg taccgtcttt
     9421 gtgagggcat atcgtatcag cgttcacgtg ttgcgtttcg tgtagagaaa aaaaagaaat
     9481 cggtcacatc cgtcgcgcgg ggtttcattg atctttggcc caaacatgag cgccttgttg
     9541 ctgaactaaa tgggccttac catcaaccac gtaccatgcc cgggcagtcg ttttattatt
     9601 ttttgcaagg tggtttgcgc attttgatcg acgggagtat cgggtcatgg cggcgtctgc
     9661 gttttgccga aaatcactcc gcttgggttt cggaagatga attgacctac gcggcacccg
     9721 ggacaccggt gcccgctagc accatacccg taattcgtgt gcgtcgcgat gaacgtacat
     9781 ccgtgatcaa acttacgatg tcggaaaaac ttccatatcg cgttgaacaa
//
LOCUS       DAOQNQ010000105         9796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2521_length_9796_cov_6.839236, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000105 DAOQNQ010000000
VERSION     DAOQNQ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9796
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2521_length_9796_cov_6.839236"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /locus_tag="PK754_07995"
     CDS             complement(<1..1461)
                     /locus_tag="PK754_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB09484.1"
                     /translation="MLRCITILWMIGYFHSGTVSSQEYGSINGFVYDAANGEAVIGAN
                     VFINGTKLGAVTNLNGYYVIPKVPAGIYLLVVQSLGYKDYTQKITVKPGDKLRVNTQI
                     HGEDIQGEEIVVTADSIPTIMKLFEEPISRIELTGTQINQIPQVAESDLLRSLQTLPG
                     ILPVSDFSSELYVRGGTPDQNLYLLDGTDVYNPEHAFGLFSTFNTDAIKQVQISKGGF
                     GAEYGGRLSSILNVTNLDGNRKEFEGSAGISLLSAKTTLQMPIGNFGSVSASIRRTYF
                     DKTLGQAIDEIPNYYFYDGNIKAFFELDSRNTLTISTYGGRDVLNFNFNENAEDKSGI
                     DYDWGNKTGSIRWTRVFNPNWFGNFWVTGSVFSSQFHLDEIVDEDNFISDITIKGNIE
                     YHQSKQFIYKLGFEQKTMHGKYQQDFPNGAVDVDAHRYLYTMYGQTNWRPNENWDIEA
                     GLRYDYFDSPRDYQFIDPRLAAKYRINETTNLKASAG"
     gene            complement(1886..3988)
                     /gene="fusA"
                     /locus_tag="PK754_08000"
     CDS             complement(1886..3988)
                     /gene="fusA"
                     /locus_tag="PK754_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNB09485.1"
                     /translation="MKTFTPDKIRNICLASHSGTGKTTLAEAMLFVSGTKKRFGSVDE
                     GSTSSDFHPDEIERKFSINTSLMHCEWKGHKINILDTPGYADFRGEVRSAMRVADVVI
                     IPIRATAMLDVGAEIAWNYAEEHNLPVIFCVNELDKENSNFDQTFDVLMERFGDGRHI
                     VEIGFPIGEGRPDFNKIFDIISMKLLKFELDKTGNYEALDVPSEFKERLNKLHEILIE
                     DAAESEEEFMEDFFATGTLTDEEFKKGLREGIQGRKIVPVFCTDGINSVGIKPLLDFI
                     VDYCPSPADMPSATGRTNTNAQEQRPNKSDAPFSALVFKTISENHVGELSLFRVYSGT
                     VKSGDDVLNASKNVTERIGQVFLLNGKEREDAGQVGAGDIAALVKLKDTHTNNTLCDK
                     SKPIVFGEIAFPDPVITFGIVPKGKADETKISQGLHTLHEEDPAFLYTYDVETQQSTV
                     SGQGEMHLTIITRRLKERFGVEVDLVDPKIPYKETIRGAVKDAEFKHKKQSGGRGQYG
                     HVHIKLSPKQRGSGFEFIDEVVGGVVPGRFIPAVEKGVVEAMTGGVVAGYPVVDVAVT
                     LYDGSYHDVDSDELSFKIAGAQAFRKGFKEGHPIILEPIYEVEITIPEEYLGAIMGDI
                     SSRRGQILGMEADGHFQIVKAQLPLAEILKYATILRSMTSGRGMFRKKFDHYAEVPHE
                     QAAKLMKEYEERKAAGNG"
     gene            complement(4265..4690)
                     /locus_tag="PK754_08005"
     CDS             complement(4265..4690)
                     /locus_tag="PK754_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6326 family protein"
                     /protein_id="HNB09486.1"
                     /translation="MIKIKEHDINIRFKLTALWTSVTLCYLYCDYFQLYAPEKVKGLM
                     SGQNMLDSPVKLLTAAVMMAVPACMVFLNLVLSDRILKVLNIVFGSFFTLIMLLIIPA
                     ILKPWYAFYVFYAVLEIVITIFIVRMAWKWDINKPAKVN"
     gene            complement(4799..5482)
                     /locus_tag="PK754_08010"
     CDS             complement(4799..5482)
                     /locus_tag="PK754_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HNB09487.1"
                     /translation="MSTARLAGFLYLVVVLTGMFTLAYVPSVLIDWNNASVTYNNIKS
                     AETLFRLGIVAGIICYTAFVLLPFVLYKLLSQVNQTFAVGMVVLSTVSVPFSLVNLLY
                     KVNVLTLIANSGNLTDPELEKTQEQIMLLLNYYGNGIQIVSIFWGLWLFPFGYLVFKS
                     GFLPKILGVFLMAGCFGYLINFTGRFLYPNYASFGIGGYVSLPATIGEIGICLWLLIG
                     GIKTQTASE"
     gene            complement(5723..7090)
                     /locus_tag="PK754_08015"
     CDS             complement(5723..7090)
                     /locus_tag="PK754_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNB09488.1"
                     /translation="MGNNITIVGAGLAGSLLSVYLAKRGYTVDLYERRPDMRQVKISA
                     GRSINLALSTRGIHALKEVGLFDEISKILIPMKGRMMHGTDGAQTFVPYGKDDTEVIN
                     SVSRGELNMRLMSLAESFPGVKIHFNQRCTGMNFETGEVFFTNESNGEKHSIFSERVI
                     GTDGSASALRSDMMRLNRFNYSQSYLEHAYKELTIPPAKNGSFRLEKNALHIWPRKTF
                     MLIALPNIDASFTCTLFLSLEGPESFSTLDTPEKVTLFFKKYFSDALEQMPDLTNHFF
                     ENPTSSLMTVKSFPWSYEDKVVLLGDASHAIVPFFGQGMNCSFEDCSELNRFIGTYSG
                     DWKKIFKEFEKSRKPNSDAIADLAVENFYEMRDLVADPRFLLKKKVEHILEEKFSDFF
                     VPKYAMVTFHRIPYSVAMERGKIQDIILDEITTGIHDPHHVDIAIAEHLIKTRLTPWS
                     DVVRL"
     gene            complement(7095..7958)
                     /locus_tag="PK754_08020"
     CDS             complement(7095..7958)
                     /locus_tag="PK754_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB09489.1"
                     /translation="MKNLIYAALWVIILVLSCRQPQTHQPLSAKDGVTIDYRVVGQGD
                     TTILFLHGWGINQTYWDQQIEHFRKKYKVVTLDLPGFGKSGKNREAWTIENYAEDVAH
                     AIKTLELQSVILVGHSMSGSIVVETAVRLPSNIIGVIGVDNFKDVGHVSLPEEKAGID
                     TFIHYLGVDFVGTITASAEGTLYSAQTDSTIRKRILNDVITGNSIVSKNVLNAVFSYG
                     SKEVEMLKKLPYKLFLINSDNQPTETKVLSQIVTHSCEVFTVHATSHYPMIENAEDFN
                     RQLETIISKLK"
     gene            complement(7979..9241)
                     /gene="kynU"
                     /locus_tag="PK754_08025"
     CDS             complement(7979..9241)
                     /gene="kynU"
                     /locus_tag="PK754_08025"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018944936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HNB09490.1"
                     /translation="MNFENSVGFAQKLDQEDSLKEFRSRFHIPCHKDGTQSIYFCGNS
                     LGLQPKSVRSYIEQELKDWETLGVEGHFHAKNPWMPYHEFLTAKAAKIVGANEHEVVH
                     MNSLTTNLHLLMVSFYRPTKERHKILIEKTAFPSDQYAVQSQIRYHGYDPKTALVEIM
                     PRDGEEILRTDDIEEYIQSHGQEIALILLGGVNYYTGQAMDMLRITAAGHNAGCVVGF
                     DLAHAAGNIILSLHDWNVDFAAWCSYKYLNSGPGGISGIYIHENHTRNHNIPRFAGWW
                     GHDKKTRFLMKPDFIPIPTAESWQLSNAPVLLMASLRASLDIFDEASMDRLRKKSLLL
                     TGYLEYLIHDIKTDNFVLITPSDSSQRGCQISIQTKRNGKKLFEKLSEAGIIADWREP
                     DVVRVAPVPLYNSFLDVYRFAEILKKHS"
     gene            complement(9245..>9796)
                     /locus_tag="PK754_08030"
     CDS             complement(9245..>9796)
                     /locus_tag="PK754_08030"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNB09491.1"
                     /translation="DKLLIPATLITIEPEKAVDSTETYAPDKQNADLIITAKSLFYKL
                     HTLPREFMSALVIRTTPNVPSKKSRDYMKENSPFFSLEAMDYLFEIGVEHLLVDTPSI
                     DRLFDEGKLSAHHVFWGVPQGSHDIHLEVHSRKTITEMIYVDNSIADGNYFINIQIPN
                     FVADAAPSRILMYQAQLKNQLLP"
BASE COUNT         2959 a         2103 c         1799 g         2935 t
ORIGIN      
        1 accggccgac gctttgaggt tcgtagtttc attaatacga tatttagccg ccaatcgcgg
       61 atctataaac tgataatctc tgggtgaatc aaaataatca taccttagac ccgcttcgat
      121 atcccaattt tcattgggcc gccagttcgt ctggccatac attgtataga gatagcgatg
      181 cgcatctaca tctacggcac cattgggaaa atcttgctga tatttcccgt gcatggtttt
      241 ttgttcgaaa cccaatttat atatgaattg tttggattga tggtattcga tattcccctt
      301 tattgtgata tcacttataa agttgtcttc atctacaatc tcatcaagat gaaattgtga
      361 actaaacaca ctccctgtga cccaaaaatt gccaaaccaa ttgggattaa atacccgtgt
      421 ccaacggatg ctgccggttt tattgcccca gtcataatct atacccgact tatcttcggc
      481 gttttcatta aaattaaaat tgagaacatc ccgtccgccg tatgtgctta tcgtaagcgt
      541 gtttcggcta tccagttcaa aaaaggcttt gatattacca tcataaaagt agtagttagg
      601 gatttcatca atagcttggc caagtgtttt gtcaaaatat gttctccgta tggaagcgga
      661 taccgatcca aaattaccaa tcggcatttg aagcgtcgtt tttgcagaaa gcaggctaat
      721 accggcacta ccttcaaatt cttttcggtt tccatcaagg tttgtaacat taagtatgga
      781 tgataatcgc ccgccatatt cagcaccgaa cccgcctttt gaaatctgca cttgtttgat
      841 cgcatcggta ttaaaggttg aaaacaaacc aaaggcatgt tccgggttat atacatccgt
      901 accgtccaat aaatataaat tttgatccgg cgtcccccct cgaacataaa gttcggatga
      961 aaagtcggat actggaagga taccgggcaa ggtctgtaaa gacctcaaaa gatccgattc
     1021 ggcgacttgc gggatttgat tgatttgtgt accggttaac tcaattcgtg aaataggctc
     1081 ctcaaataac ttcataatgg taggtataga gtccgccgta accactattt cttcaccctg
     1141 aatatcttcg ccatgaatct gagtatttac acgaagtttg tcaccgggct tgactgtgat
     1201 cttttgcgtg tagtctttgt aacccaaaga ttgaacgact aataaataaa tgccggcagg
     1261 aaccttggga ataacataat atccgttgag gttggttacg gcacctaatt tggttccatt
     1321 gataaaaaca ttagcaccga tcacagcctc cccattggct gcatcatata caaaaccatt
     1381 gatcgaacca tattcctgag atgataccgt tcccgaatga aaatatccta tcatccataa
     1441 gatagtaata caacgtaaca ttggactcct aaacaattta actttataca aaataaaaat
     1501 gattaaatgg gttttatatt caaaatgaga taaagaactt cgcaaaaacc tgctacttac
     1561 acgttattat gtcttttaag tttcgtcaat tctcgagtta ttacgatacg acagaaatac
     1621 aaacaaacaa taaaaggtaa atcaaaaatt gatatgttct tatcaacact cgctaaaaaa
     1681 aatctttttt gatgccgatc ttcaatatca agctgaagat cgggttaaaa ctatcattat
     1741 tcattgattt aaatcaataa acccgatact tatcttgtaa agacaaaaag ccgcctcaga
     1801 aatatctcta attatatatc acaatgaaag caatataaat tgatacttct gacgcggctt
     1861 tggatactat actgattata aatatttatc cgtttccggc agctttacgt tcctcatact
     1921 ctttcataag tttagcggcc tgttcatgag gaacttcggc gtaatggtca aattttttac
     1981 ggaacatacc acgaccacta gtcatagagc gcaatattgt agcatattta aggatttctg
     2041 ctaaaggtaa ttgcgctttg acgatctgga agtggccgtc ggcttccatt cccaaaattt
     2101 gccccctacg tgaagaaatg tcacccataa tcgcaccaag atattcttca ggaatggtta
     2161 tctccacttc atatatcggc tctaaaataa tcggatgacc ttctttgaaa ccctttctaa
     2221 atgcttgagc acctgcaatt ttgaaggaaa gttcatcaga atctacatcg tggtatgaac
     2281 catcatacaa agtaaccgct acatccacga ccgggtaacc cgccacgaca cctccggtca
     2341 tcgcttcgac cacacctttt tctaccgccg ggataaagcg ccccggaaca acaccaccaa
     2401 ccacttcatc aataaattca aacccgctac cgcgttgttt gggtgaaagt ttgatgtgta
     2461 catgcccgta ttggccgcga ccaccggatt gttttttatg tttaaattca gcgtctttga
     2521 ctgccccccg aatggtttct ttataaggaa tcttcgggtc aaccaaatcc acctcgacac
     2581 caaaacgttc tttcaatcgg cgggtgatga tcgtcaagtg catttcacct tggccactga
     2641 ccgtgctttg ttgcgtttca acgtcgtagg tataaagaaa tgccgggtct tcttcgtgaa
     2701 gtgtatgtaa accctggctt attttggttt catcagcttt tccttttggc acaataccaa
     2761 aagtaattac cggatccgga aatgcaattt ctccgaaaac gatgggttta gacttatcac
     2821 acaacgtatt attggtatga gtgtctttca gttttacaag cgcagctata tcgcccgcac
     2881 ccacctgtcc ggcgtcttca cgctcttttc cgtttagtaa gaaaacttgc ccgatgcgtt
     2941 ctgttacgtt tttactggca ttgagtacat cgtcacccga cttgaccgta ccggaatata
     3001 cacggaaaag tgaaagttca ccgacatggt tttcactaat tgttttaaaa accaacgctg
     3061 aaaagggagc atctgattta ttaggacgtt gctcctgagc attggtattg gtgcgaccgg
     3121 tagcggaagg catgtccgcc ggagaagggc aataatccac tataaaatcc aataacggtt
     3181 tgatgccaac cgaattaatg ccatctgtac aaaatacggg tacgattttt ctgccctgaa
     3241 taccttcacg taatcctttt ttgaattctt cgtctgtcaa tgtaccggtt gcaaaaaaat
     3301 cctccataaa ttcttcttcg ctctcggccg catcttcgat aagaatttca tgcagtttgt
     3361 ttagccgctc tttaaattcg ctcggtacat ccagagcttc ataattaccg gttttatcaa
     3421 gctcaaattt cagcagtttc atgctgatga tgtcaaaaat tttattaaaa tcagggcgcc
     3481 cctcgccaat aggaaaacca atctctacga tatgccgccc gtcaccgaag cgttccatca
     3541 agacatcaaa agtctgatca aaattggaat tttctttatc caattcgttt acacaaaaaa
     3601 tcacaggcag attgtgttct tcagcatagt tccaggcgat ttcagcgccg acgtccagca
     3661 tggccgtcgc acgtataggg ataatcacca cgtcggcgac acgcatagct gatcgcacct
     3721 cacctcgaaa atcagcataa cccggcgtat ctagaatatt gatcttatgg cctttccatt
     3781 cgcaatgcat taaagacgta ttgatagaaa atttacgttc gatttcatcc gggtgaaaat
     3841 cactggatgt cgagccctca tccacactac caaatctttt tttggtgccc gatacaaaca
     3901 acatggcctc ggccaacgtc gttttacctg tgccgctgtg agatgccaaa caaatattac
     3961 gaatcttatc aggcgtgaag gtcttcatac cgcatgtctc ctaaaagtgg tgtacgattt
     4021 cggctctaat gcgaaaggac aatatcaaaa gcctgtaaaa ggaaagcact aaaatctata
     4081 aaaagtatga ctaaagtcaa agatcgaaga tattgataaa catttaactg atcttgatgt
     4141 atcaacttca aaaatcgatt aaatcttatt aaagagactc actagaattg attcaaaagt
     4201 aggcgggata ttttaaaagt aaaccaacca aaaaatagat ttgaaatatt ttttgatttc
     4261 agcgtcaatt tactttagcc ggtttgttga tatcccactt ccatgccatt cgaacaataa
     4321 atattgtaat gacgatttcc agcacagcat aaaacacata aaacgcatac caaggtttta
     4381 gtatagccgg tataataaga agcatgatca gagtaaaaaa agaaccgaaa acaatattca
     4441 aaaccttcaa aatccgatcc gacaaaacaa gatttagaaa taccatacaa gccggaacgg
     4501 ccatcatcac tgctgctgtt aatagcttta ccggactatc aagcatgttt tgaccgctca
     4561 tgagaccttt gactttttct ggcgcgtaca actgaaaata atcacaatac aagtaacaaa
     4621 gggttacgga tgtccacaat gctgtaagtt taaatctgat attgatatca tgttctttta
     4681 ttttgatcat tgtttcaact tcatcgcttt agatttgaat tgttgttttt atttataaat
     4741 atattttcaa ataaacgtgt tatcatgatt taataaaggc ttacgaacgt ctggatattt
     4801 attctgatgc tgtttgcgtt ttgattccgc caatcaacaa ccaaaggcat attcctatct
     4861 ctccgatagt cgccggaagt gaaacatatc cgccaattcc aaaactggca taatttggat
     4921 acagaaagcg gcctgtaaaa ttgataaggt acccaaaaca acccgccatt agaaaaacac
     4981 caaggatttt cggaagaaag ccggacttaa aaaccaaata cccaaatgga aaaagccata
     5041 atccccaaaa gatggaaacg atttgaattc cgttaccata gtagttaagc agtaacataa
     5101 tctgctcttg tgttttttcc agttcagggt ctgttaaatt tcctgaattt gcgatcagag
     5161 ttaagacgtt gaccttatac aaaagattta cgagagaaaa ggggacgctt accgtagata
     5221 acaccaccat accaacggca aatgtttgat ttacttggct cagtagttta tagagtacaa
     5281 acggtaacaa tacaaaagct gtgtagcata ttattccggc cacaatacct aaccgaaata
     5341 gcgtttctgc gctttttata ttgttataag taaccgaggc attattccaa tcaatgagta
     5401 cagagggcac ataggctaag gtaaacattc ccgtcaacac taccactaaa tataaaaatc
     5461 cggccaagcg tgctgtactc atacattctc tccattttca tcaaattaaa atcttacacg
     5521 gattttttgg atatttgttt ctttccctaa ccttcaaatt agtgattggc tctttaatga
     5581 ggaatgtagt tcgctatgta acgcgcggga gtttgtatga tacgatggga ggttgttcct
     5641 gttaatcaag ttaacagtaa gtcaacaggc aagtggtaaa atattaagct aaacgataaa
     5701 ctttaatagt ttgaaaattt ttctagagcc taactacatc cgaccaggga gttagtcttg
     5761 ttttgatcaa gtgttctgca atggcgatat caacatgatg agggtcatga atgccagtag
     5821 ttatttcatc caaaataata tcctgaattt ttcctcgttc catagcaaca gaatacggaa
     5881 tacggtgaaa tgtaaccatt gcatatttag gaacaaaaaa gtccgaaaat ttctcctcta
     5941 atatatgttc aacttttttc ttcaatagaa accgagggtc cgcgactaaa tcacgcatct
     6001 cataaaaatt ctcgaccgca agatcggcga tcgcatcgga atttggtttg cgcgattttt
     6061 caaattcttt gaaaatcttt ttccaatctc ccgaataagt gcctataaaa cgatttagtt
     6121 cagaacagtc ctcgaaagaa caattcatcc cttgaccaaa aaacggaaca atcgcatgcg
     6181 atgcgtcacc cagaagtacg actttatctt catagctcca cgggaaactt ttgacagtca
     6241 tcagagaact ggtcggattt tcaaaaaagt gattcgttaa atccggcatt tgctctaatg
     6301 catcggaaaa atatttttta aaaaataaag tcactttttc tggcgtatcc aaagttgaaa
     6361 aactttccgg cccttccagc gataaaaaca atgtacaagt aaaactcgca tctatgttgg
     6421 gaagtgcgat cagcataaac gttttgcgtg gccaaatatg tagcgcgttt ttctcaagtc
     6481 gaaacgaacc gtttttggct ggaggtatag ttagctcttt gtaggcatgc tcaagatagg
     6541 attgagaata attaaatcta tttaggcgca tcatatcaga ccgaagggca gaggcggatc
     6601 catcggtgcc gataactcgt tctgaaaaaa tgctgtgttt ttcaccgttg gattcattcg
     6661 taaaaaaaac ttcgcctgtt tcaaaattca tccccgtgca acgctgatta aaatggattt
     6721 tcacgccggg aaacgattcc gccaaagaca tcaaacgcat attgagttcg cctcgtgata
     6781 cggagttgat cacttccgta tcatctttac catagggcac aaaggtttgt gcaccatcgg
     6841 taccatgcat cattcgtcct ttcatcggaa ttaatatttt actgatttca tcaaataacc
     6901 cgacttcttt aagcgcgtga atacctcgtg tagataaagc gagattgatg gaacgcccgg
     6961 cgcttatttt gacttggcgc atatcgggtc gtcgttcgta taaatctaca gtataacccc
     7021 gcttcgcaag gtaaaccgat aaaagtgaac ccgccaatcc ggcgccgacg atggtaatgt
     7081 tgttgcccat agtattattt taattttgag ataattgttt ccagttgtcg gttaaaatct
     7141 tctgcattct caatcatcgg gtaatgactg gtcgcgtgaa cagtaaacac ctcacaagaa
     7201 tgagtgacta tttgagaaag taccttcgtc tccgtcggct ggttatcact gtttatcaaa
     7261 aacaatttat acggcagttt ttttaacatc tcaacttctt ttgaaccata tgaaaaaaca
     7321 gcgttcaata cattttttga aacaattgag tttccagtga taacatcatt caaaatccgc
     7381 ttgcgaatcg tcgaatcagt ttgtgctgaa tataatgttc cttctgcgga tgctgtgatc
     7441 gtccccacaa agtctacacc caaatagtgt ataaatgtat ctataccggc cttttcttcc
     7501 ggcaaggaaa cgtgaccaac atctttaaaa ttatccactc cgataacacc aatgatgttt
     7561 gaaggcaaac gaacggctgt ttcgacaact atactacccg acatcgaatg tcctacgagt
     7621 ataacgcttt gtagttccaa tgttttaatc gcgtgagcaa cgtcttcagc gtaattttca
     7681 atagtccatg cttcacgatt ttttccggat ttgccaaaac ccggcaaatc taaagttacc
     7741 accttatatt tttttctaaa atgttctatt tgctgatccc agtaggtctg atttataccc
     7801 catccatgaa gaaacaggat tgtcgtatct ccctgaccga ctactctata atctattgtt
     7861 acaccgtctt tcgctgataa cggttgatgg gtttgtggtt gacgacaaga caaaaccaaa
     7921 ataatgaccc acagcgctgc atagataagg tttttcatac taatttgcct tttattaatt
     7981 aggagtgctt tttcaaaatt tcagcaaatc tatagacatc aagaaaggaa ttatacagcg
     8041 gcaccggtgc aactcgaaca acatcaggtt cacgccagtc tgcgattatc cctgcttcac
     8101 tcagtttttc aaatagcttt tttccgtttc gtttggtttg tattgaaatt tgacagccgc
     8161 gctgagaaga atccgatgga gtgatcaaca caaaattatc ggtttttata tcatgaatca
     8221 aatattcaag ataacctgta agaagaaggc tcttttttct taggcgatcc atactcgctt
     8281 catcgaatat atccaaagat gcacgcaatg aagccatcaa taacacgggc gcattactta
     8341 gttgccaaga ttccgccgtt gggattggta taaaatcagg tttcattagg aatcttgttt
     8401 ttttatcatg cccccaccat cccgcaaagc gaggtatgtt gtgattacgt gtatggtttt
     8461 cgtgtatgta tattcctgat attcctccgg gaccggaatt cagatattta taggaacacc
     8521 atgccgcaaa atccacgttc caatcgtgta aactcaaaat aatattacct gcagcatgtg
     8581 ccaaatcaaa tcctactaca caaccggcat tatgccctgc tgctgtaatg cgtagcatat
     8641 ccatggcctg tcctgtgtag taattaactc cacccaataa aatcaaagct atctcttgtc
     8701 cgtgagattg aatatactct tcaatatcat ctgttcgtag aatttcttcg ccatctcttg
     8761 gcattatttc tactaacgct gttttaggat cataaccatg atagcgaatc tgtgattgta
     8821 cggcgtattg atcggatgga aacgctgttt tttctatcag tattttatgt ctttctttgg
     8881 tcggcctgta aaacgaaacc atcaatagat gcaaattagt cgttagcgaa ttcatatgca
     8941 ccacttcgtg ttcattagca ccgactattt ttgccgcttt tgcagtcaaa aattcatgat
     9001 aaggcatcca cggatttttg gcatgaaaat gcccttcaac tccgagggtt tcccaatctt
     9061 tgagctcttg ctcgatataa gaacgcacgg atttgggttg aaggccaagc gagttaccgc
     9121 aaaaataaat ggattgagtt ccgtccttat gacagggaat atggaaccgt gaacgaaatt
     9181 cttttaagga atcttcttga tctaattttt gagcaaaacc aacgctgttt tcaaaattca
     9241 tattttatgg tagtaattga ttttttagtt gcgcctgata catcaaaata cgacttggag
     9301 ccgcatcagc aacaaagtta ggaatctgta tgtttatgaa ataattaccg tcggcaatac
     9361 tattatcaac atatatcatt tcggtaatgg ttttgcgtga atgcacctcc aggtgaatat
     9421 cgtgtgatcc ttgaggcact ccccaaaaaa catgatgcgc tgacagcttg ccttcatcaa
     9481 ataaacgatc tatcgaaggc gtatccacta gtagatgctc tacaccgatc tcaaaaagat
     9541 aatccattgc ctctagagaa aaaaatggag agttctcctt catataatcg cgcgattttt
     9601 tggatggaac gtttggcgtt gtccgaataa caagggccga cataaattca cgaggcaaag
     9661 tatgaagttt ataaaaaaga cttttagctg tgatgataag atccgcgttc tgcttatccg
     9721 gtgcatatgt ctcggtcgaa tccacagctt tttccggttc gatcgtaatg agtgttgcag
     9781 gaatcaaaag tttatc
//
LOCUS       DAOQNQ010000106         9668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2579_length_9668_cov_5.388952, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000106 DAOQNQ010000000
VERSION     DAOQNQ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9668
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2579_length_9668_cov_5.388952"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..637
                     /locus_tag="PK754_08035"
     CDS             113..637
                     /locus_tag="PK754_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09492.1"
                     /translation="MFSRLYSLLLLLLLLSLPFTQPVSAQVTVNRKEFKCTDQQIFAT
                     YDLLKTTRSYGNYDPQNEWSQELSSDQIIRKSVELEGGRQYAILLVTRKDVDATGVEI
                     RDQYGFQLEYVYKVTELDKGQINVFYTPDVDGIYQLYFRVVDNAPVCSYMAVLKGDLD
                     PEFGIDEDEDGNSK"
     gene            complement(665..1282)
                     /locus_tag="PK754_08040"
     CDS             complement(665..1282)
                     /locus_tag="PK754_08040"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09493.1"
                     /translation="MSNHFSWNNNFSAYNTAIASVSWEQTVWKLKLSAQFFSVKNYLY
                     WNSDALPAQYDRVLKAYSFFLQKNFVYKGFHLENELQYQSYSNDTVVSFPTFLLRQSF
                     YFENRLFKGALHSKIGIDIRYNNDYFAPAYMPETGQFYQQQFSKLHYYPVADLFISLQ
                     VKGVRAFAMMQHINKDMFGPGYFAVYRSPMPDRSFKLGLEWRFWN"
     gene            complement(1302..1829)
                     /locus_tag="PK754_08045"
                     /pseudo
     CDS             complement(1302..1829)
                     /locus_tag="PK754_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258061.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(1906..5433)
                     /locus_tag="PK754_08050"
     CDS             complement(1906..5433)
                     /locus_tag="PK754_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNB09494.1"
                     /translation="MHVQRTILIVLGFLMIPFSLTAQTPQVELQLQPPDGVGQMVFSP
                     DGKWLAMGASDIRLLHIPSQKIINLGYHKNRVLCLAISPNGRYLASGDGKGDIYVWDM
                     LQSKMASVLHDTTGSQKILFSEDGANIIACQSFDVHIWNLAQRKKIKSYPFFDAQEKI
                     RLFPNSDCSVFDYHGISPDGKWAAIAGNTSCLVSRTTEGGYKLPGFDFNQFFQAINLE
                     TGEILEKIRIPKDMDLGAVALGRQKLALVVSTDKTTPATYKALIYNLETQSIYRQINA
                     DTNRIVHLALSPDESKLVGVSAFINALQWAQGSVLMWDIPTSRLIGTNKMAYHKTVFS
                     ADGYWLAGGFDTYLLAAPVKNFPRGVYTMGPEYSANVYDFVLDEDRGKILFSDREHVM
                     TWDIKRGVLSQKFVGSGDQTIRGVAFTAKGILTAGFRGSIVHWDSSGKNQIARMQHPV
                     LNLKNITRIDDSTYASYGTGIPSLNQDTLRFWNVYNGALTRIQTAHSDKINGMRRSPD
                     EKIWVTFGGEEKNSPSGSVCVWDATQWTKTGDYHISDQTTEVIQVLIYPDNKTALIAT
                     DHAERIVMNLHDGSIIRRMNDSGNYAVTMEFSPDGALYACGNGGPWLLGSSDYHVRIY
                     DAESHRLVKILKGHTNKIEKLQFTKDGRTLVTGSLDGTIKFWNLSNQEVTSLIGVSED
                     TYVLFTENGYYMTSKAGRDLVAFRSGMKLMTFDQFDLAYNRPDVILARLGHTPPEQLN
                     LYKQAYEKRRLRNEGVVAAALIRDDAPDIDVTTDTPPETNTSAMPLKVKIRDAKNNLK
                     TLKVMVNDVPVYGRAGLDLRGRNAQNYETDMAITLSEGKNRIQISAINTAGVESIPET
                     FETTFSGWPEPTDVICVSVGIANYQDPDYNLTYSRKDAEEMAAAFETMQKNQRGIFEK
                     IILTDTSAVKENILAIKKRLMQTRPNDRVILFFAGHGLLDEKLDWYFATYDTDFRNPS
                     KRGLGYSEIENLLDGIPARRRLVLMDACHSGEVDKETSAPVMTLVDGGPSSVSARGLK
                     QKIGIGENPVQNVNSRGFLAKKTGAVGLQNSFDLMRELFADLQNGIGATVISSASGTE
                     LALEGGEWKNGVFTYSVLQGLTNGKADLDHDGIIRCSELQTYVTQSVKQLTQGKQTPT
                     ARKEKMLDNFEVVKFTGPIDPSLWRKPGY"
     gene            complement(5454..8333)
                     /locus_tag="PK754_08055"
     CDS             complement(5454..8333)
                     /locus_tag="PK754_08055"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09495.1"
                     /translation="MKSTIVFVLFLSSVLYAQRTGCVSGNCENGEGTYVWSSGTTYAG
                     EWKNGKQHGKGKTIWSDGEWYEGGYNMGERTGWGEFHWKDKSWYRGGYSNSRMHGFGE
                     YHYANGATYTGQMRLGKFSGVGTKLKNDGAREIGFWKNDTYMGSQRPVLVVSSQGPIR
                     SIQAAIDSATSGDEILIRRGTYNEAIVIDGKENITIRGEGEVWLKVPTGDIISIRNSS
                     GIALFNLKGIHDTRDEYFSCETSDVLDIVFSKNIIVAQCLLDGSGQIGLTGYHVEGIL
                     VSHTIVQNCIFGGITLENCTDVTLQYNLIIAQKAAVDKSMDCIGLSLVSCKNAVVNHN
                     TVAKLIDFNPVWIRDSESILFTNNIISHNTLNAENQSGLMLGENQTLDYHRNLLWDNK
                     TRDGKEINYDAYAKSFKPLDDINTDPLFLNPQSKQFGLKTSSGALNQATDGLDIGAVQ
                     TKHRGNLTPKITDQTPPLIVWSGQNFTDKQNIPLSDNMIKFSGRLTDNTELAGMVIGN
                     KTVSLKTDGAFNAEIVLEDDMGMIPVRAWDIFGNAAYLDIFYQKSSTPTASVQTGTVP
                     QTITANRPTTSSSAVKSSSATPNTSTVTVTSDCKTSDECIERSFKAQSIEEAILYAES
                     AVRLLKPTDEEVTQKMAYRIRAETYLYQFQKSNDAALVGKIEADFEKAIKLDPQDGVP
                     YIGLAKLYETQNQELMVEDQFARGLQANASPALLYYERSQYYIRQKKESAADEDLDKA
                     IEMLGGTSYKDGTYKITAGDPSVTDKGKSNMYYTRAERRMAREDYKSAYDDFTMALKY
                     NPQSSNAYWNRAKIIIHIDPYFKKPEPALADLNKVIELSPDFADAYYWKGQALLANSQ
                     TYTDHTATIETLVKAAELDATFSDGYAYASQLCLVNERHTEAERFAFLAVQKTDKKNA
                     LGLAHYVLGYTYSYKRDRENMLNHFRKSVEYGYEESRNVLKSMGY"
     gene            8746..>9668
                     /locus_tag="PK754_08060"
     CDS             8746..>9668
                     /locus_tag="PK754_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09496.1"
                     /translation="MRTKLLLLASAFLFLSGCAQLGELAGNLMTQSTTDLQACGTTVY
                     YISNLYPAAVKSTETSYMGEAWKEGRSAVTVMNFKKHGIGMYKIDGIVAMDGTPMSHV
                     AFGAYTKLVDSGDTSPKKITIETKTGQKAEFTIKPTYPVRIKSINGKADGAEIDVTQD
                     LVLEMENPKGSERTMYKVGMLMDLMGTTSFVDVVVCRSADKIVIPHQAFVNAQVVASS
                     DQAIGTSIASFREGASWLRVERYEHDLSPNTGTGGYQGISASWDTKPVTVKGKSPSKS
                     NVQITGEEKGFSFTANKPNAYYGRALSMGKK"
BASE COUNT         2503 a         2199 c         2163 g         2803 t
ORIGIN      
        1 gcattacatc ccaattcacc gggttacagg ctcatcctga acaatgtgct tttccctgcc
       61 gcaaaaaaga aaaagcaaaa aacgtaggtt gtcttcaact atctttgcgc tcatgttcag
      121 ccggttgtat tcacttctcc tgttattgct attactcagt ctgccattta cacaacctgt
      181 atcggcacag gttaccgtga acaggaagga atttaaatgc acggatcaac agatttttgc
      241 tacttacgac ttgctgaaga caacccgtag ttacggtaat tatgatccac agaatgaatg
      301 gagccaggaa ctttcatcag atcagatcat caggaaaagc gtggaactgg aaggaggcag
      361 gcaatatgcc attttactgg ttacacgcaa agatgttgac gcaacaggtg ttgaaatccg
      421 tgatcagtat ggatttcagt tggaatatgt ttacaaggta acggagctgg ataaaggaca
      481 gattaatgtg ttttacacac ctgatgttga tggcatttac cagctgtact tccgtgtagt
      541 ggataacgcg cctgtatgct cctatatggc tgttttaaag ggtgatttag atccggaatt
      601 cggtattgat gaagatgagg acgggaacag caaataagtt atccgtattt acggttgtca
      661 gccattaatt ccagaatcgc cattccaacc ccagcttaaa tgacctgtcc ggcataggtg
      721 aacgatacac tgcaaagtaa ccgggcccga acatgtcctt gttaatatgc tgcatcattg
      781 caaaagccct tacgcctttt acctgcaacg aaataaataa atcagccacc ggataataat
      841 gcagcttgct gaattgctgc tgataaaact gaccggtttc cggcatatag gcaggagcga
      901 aataatcatt gttataacgt atgtcaatcc cgatcttgct gtgcaaggcg cccttaaaca
      961 gacggttctc aaaataaaaa ctctgccgca acaggaatgt cggaaagctc acgacagtat
     1021 cgttgctgta agactgatat tgcaattcat tctccaggtg aaatcccttg tacacaaagt
     1081 ttttctgaag aaaaaaagaa tacgctttca gcacacgatc atactgcgcc ggcagtgcat
     1141 cgctgttcca gtataaatag tttttgactg agaagaactg agcggataat tttaatttcc
     1201 ataccgtttg ctcccaggaa acagaagcaa tagcagtatt gtaagccgaa aaattattgt
     1261 tccagctgaa atggtttgac aaatagtgtt gctgaatgta ggcacaaagc gacgcgacgg
     1321 cgttcaccgc cggaaagtac tttgacgggt gtgtcgcttt ccgggcaacg cagtgcatcc
     1381 atagccattt caagacggct gtcaagatcc catgcgtttt tatgttcgat ctcttcctgc
     1441 agtttgcctt gtttttctaa aagcgtatca aaatccgcat taggatcggc catttgttcg
     1501 gctatttttt cgtattcacg taccaaagct accgtgtcgg caaccgcttc ctgaacaatc
     1561 tctttgactg ttttattcgg atcgagttcc ggttcctgtg gcagataacc gaaggtgatg
     1621 cctttctgcg ctgtgatttc accgaggtaa tccttgtcgg ttcctgcgat gatacgaagc
     1681 agcgtagatt tacctgcgcc gttgagaccg agtacgccga ttttggcgcc gtaaaaaaag
     1741 gacagataaa tatctttgag cacttgtttg ttgggcttgt gcactttgcc cacgccgacc
     1801 atcgaaaaaa taatcttatt gtcgctcata tccgctcgtt aaaattgata aaataaatac
     1861 cgtgtttttt aaatcgcccc taagttacac ttagaacgca gaaaatcaat acccgggctt
     1921 tcgccatagc gatggatcta tcggtccggt aaattttacc acttcaaaat tatccaacat
     1981 tttttcttta cgggcggtgg gcgtttgttt tccttgagtc agttgtttca cggactgggt
     2041 tacgtaggtt tgaagttccg aacaccgtat gatgccgtcg tgatccagat cagctttacc
     2101 gttggtaagc ccttgcagaa cgctgtatgt aaaaacgccg tttttccatt cgccgccttc
     2161 aagagccaat tcggttcctg aagcggagct gatcacggta gcgccaatgc cgttttgcag
     2221 gtcggcaaaa agttcacgca tcagatcaaa cgagttttgc aatcccaccg ccccggtttt
     2281 tttggccaaa aaacctctgg aatttacgtt ttgcaccggg ttttcaccga tgccgatttt
     2341 ttgtttaaga ccgcgcgctg agacggaaga tggtccgccg tctactagtg tcataaccgg
     2401 cgccgatgtt tctttatcca cttcaccgga atggcatgca tccatcaaaa ccaatcgtcg
     2461 ccgtgcggga ataccatcga gcaaattttc aatttccgaa tatcctaaac cacgtttaga
     2521 tggatttctg aaatcggtat cgtaagttgc aaaataccaa tcgagttttt catccagcaa
     2581 tccgtgacct gcgaaaaaca aaatcacacg atcgttggga cgggtttgca tcagacgctt
     2641 ttttatggcg agaatgtttt ctttgacggc ggaggtatct gtaagtatga ttttttcaaa
     2701 aatgccgcgc tgattttttt gcatcgtttc aaaagccgcc gccatttctt ccgcatcttt
     2761 ccgtgagtat gtaagattgt agtccgggtc ctgatagttg gcaattccga ccgatacgca
     2821 tatcacatcg gtgggttccg gccatccgga aaaagtggtt tcaaaagttt cgggaatgga
     2881 ttcgacgccg gcggtattga tagcggaaat ctgaattctg ttttttcctt cactgagcgt
     2941 gatcgccatg tcggtttcat aattttgtgc atttcgaccg cgtaagtcca gacctgcgcg
     3001 cccatatacg ggcacatcat tgaccatgac ttttagtgtt ttcaaattat ttttagcgtc
     3061 acggattttt actttcaacg gcattgcgga ggtattggtt tcaggcggcg tatctgtcgt
     3121 cacatctatg tccggagcgt cgtcgcgtat cagcgctgcc gctacgacgc cttcatttcg
     3181 taagcgtcgc ttttcgtagg cttgcttgta taagttcaac tgttcgggcg gagtgtgacc
     3241 caatcgtgct aaaatcacat cgggacggtt atacgccaga tcaaattgat caaaggtcat
     3301 cagcttcatc ccgctgcgaa acgccactaa atcgcgtccg gctttggatg tcatatagta
     3361 tccgttctca gtaaagagga catacgtgtc ttctgatacg ccgatgagcg aggtcacttc
     3421 ctgattggac aaattccaga atttgatggt accgtccaaa ctcccggtta caagagtgcg
     3481 cccgtctttc gtgaattgaa gtttttcaat tttgttggta tgacctttta atattttcac
     3541 caaccgatgg gattctgcat catagatgcg tacgtgataa tccgacgaac ccagaagcca
     3601 tggtcccccg ttaccacagg catacaaggc gccgtccgga gaaaattcca tagtaacagc
     3661 atagtttccg ctatcattca tacgccgaat tatggagccg tcatggaggt tcatcactat
     3721 acgttcggca tgatccgtag cgatcaatgc cgttttattg tccggataga tcaacacctg
     3781 aattacttcg gtcgtttgat cgctgatatg ataatcgccg gttttggtcc attgggttgc
     3841 gtcccacacg cagacggagc cgctcggaga gtttttttcc tccccgccga aagttaccca
     3901 tatcttctca tccggtgacc tgcgcatacc gttgatttta tcggaatgcg ctgtttggat
     3961 gcgtgttaac gcgccgttat acacattcca aaaacgcaag gtatcctgat taagactcgg
     4021 tatcccggtt ccatatgatg cgtaggtcga gtcatcaata cgcgtaatgt ttttcaaatt
     4081 cagtaccgga tgctgcatac gtgcgatctg gtttttgccg gaggaatccc aatgcacgat
     4141 cgaaccgcga aacccggccg taagaatacc tttggctgta aacgctacgc cgcgtatcgt
     4201 ctgatcaccg ctccctacaa atttttgact cagtacaccc cgtttgatgt cccatgtcat
     4261 gacgtgttcg cgatccgaaa agagtatctt gccccgatct tcatcgagga caaaatcgta
     4321 cacattagca gaatattcgg gtcccattgt atatacgccg cgcggaaaat ttttgaccgg
     4381 cgcagcgagc aggtatgtat caaaaccacc ggccaaccaa tatccatccg cggagaacac
     4441 cgttttatgg tatgccattt tattcgttcc gataagccga gaagtcggaa tatcccacat
     4501 tagtaccgaa ccctgcgccc attgcaatgc attgataaag gctgaaactc cgacgagttt
     4561 ggattcgtcc ggagagagtg caaggtgtac gatacgattg gtatcggcgt tgatttgacg
     4621 gtaaatactc tgcgtttcaa gattgtatat cagcgcctta tatgtcgcag gtgtggtttt
     4681 atcggtggat acgaccagcg ctaatttttg acgacccagc gccacagccc ccagatccat
     4741 gtctttcgga atacgaattt tctcaagtat ttcgcctgtt tcgagattga tcgcctggaa
     4801 aaattgatta aaatcaaaac cgggtaattt atacccgcct tctgtagtgc gcgaaaccag
     4861 acaactggta ttcccggcga tcgccgccca cttgccatcc gggctgatgc cgtgatagtc
     4921 aaataccgaa caatcgctgt ttggaaacaa acgaattttt tcctgcgcat caaaaaaagg
     4981 ataggatttt attttttttc gttgggcaag attccatata tgcacatcaa aactctggca
     5041 tgcgataata ttggctccat cctctgaaaa aagaatcttt tgtgatcctg tcgtatcatg
     5101 gagaaccgat gccatcttgg attgcagcat atcccatacg tagatatcgc ctttgccgtc
     5161 tccggacgcc agatagcgac cattagggct gatcgcaagg cacaatacgc ggttcttatg
     5221 ataaccgaga ttgatgattt tttgagatgg tatatgcaga agacgtatat ccgaagcgcc
     5281 catagcaagc cacttaccgt caggactgaa gaccatttgt cctacgccat ccggtggttg
     5341 taattgtaac tccacctggg gcgtctgggc cgttaaagaa aacggtatca tcaaaaaccc
     5401 caacacaatt agaatcgtac gctgtacgtg catggctttg ctcccgtttg cctttaatat
     5461 cccatacttt ttagaacatt ccttgattct tcataaccgt attcgacaga ctttctgaaa
     5521 tggttgagca tgttttcgcg gtcacgttta tacgaatagg tataacccaa aacgtaatgc
     5581 gcaagaccca atgcattttt tttatcggtc ttttgtacgg ccaaaaaagc aaaccgctcg
     5641 gcttcggtgt gacgctcatt gacaagacag agctgcgaag cataggcgta tccgtcagaa
     5701 aacgtcgcat ccagctccgc cgctttgaca agggtttcta tggtcgcggt atgatccgta
     5761 taggtttgcg aattggctaa caacgcctgg cctttccaat aataagcatc agcaaaatcc
     5821 ggtgagagct cgatcacctt gttgaggtcg gcaagtgcag gttccggttt tttgaaatag
     5881 gggtctatgt gaatgatgat tttggcgcga ttccaataag cattactgct ttgcggatta
     5941 tatttgagcg ccatcgtaaa atcatcatac gcacttttgt agtcctcccg agccatccgt
     6001 cgttcggcgc gggtataata catgttagat tttcccttgt cggtgacgga aggatcgccg
     6061 gcggttatct tatatgtacc gtctttatat gaggttccgc ccaacatttc gatggctttg
     6121 tctaagtctt catcagccgc cgattccttt ttttgtcgta tataatattg ggaacgttca
     6181 taatacaata gcgcaggact ggcgttggct tgcaacccgc gcgcaaattg atcttcaacc
     6241 atcaattctt gattttgtgt ttcataaagt ttagcgagtc ctatatacgg cacaccgtcc
     6301 tgagggtcta acttgatggc cttttcaaaa tccgcttcga tttttccaac aagcgccgca
     6361 tcgttgcttt tttggaattg atacaagtat gtttcggcac gaatacgata ggccattttt
     6421 tgagttactt cttcatcggt cggctttaaa aggcgtacgg cgctttcggc gtataaaatc
     6481 gcttcttcga tggattgcgc tttgaacgac cgctcaatgc attcgtccga agttttgcaa
     6541 tcggaggtta cagttacggt cgaagtattg ggtgtcgcgg aagaagattt taccgccgat
     6601 gaagaagttg taggccggtt agcggttatc gtttgaggca cggttccggt ttggacggat
     6661 gccgtaggtg ttgatgattt ttgataaaat atatcaaggt aggccgcgtt tccgaagata
     6721 tcccatgcgc gtacgggtat catgcccatg tcatcttcta aaacgatttc tgcattaaaa
     6781 gccccgtctg tttttaaaga tacagtttta ttgccaatga ccattcctgc taattccgtg
     6841 ttatccgtca gtcgtccgga gaattttatc atattgtctg acaatgggat gttttgttta
     6901 tcagtaaaat tttgcccaga ccatactatt agcggtgggg tttgatcggt tattttaggc
     6961 gtcagattgc cgcgatgttt ggtttggaca gcgccgatat ccagtccgtc cgttgcttga
     7021 tttagcgcac ccgatgaagt cttgagacca aattgtttgg attgaggatt taaaaataag
     7081 gggtcggtat ttatgtcatc taggggttta aacgatttag catacgcatc ataattaatc
     7141 tccttgccgt cccgggtttt attatcccaa aggagatttc ggtgataatc taaagtttga
     7201 ttttcaccga gcatcaaacc gctctgattt tcggcgttaa gtgtattgtg gcttatgatg
     7261 ttattggtga aaagtataga ttccgaatcg cgaatccata ccggattaaa atcaataagc
     7321 ttggcgaccg tattgtggtt gacgacggcg tttttacatg agacaagcga taaaccgata
     7381 caatccatgc tcttatcaac ggcggctttt tgtgcgatga tcagattata ttgcagtgtt
     7441 acatcggtgc agttttcgag ggttatacct ccgaaaatac aattttgtac gatcgtatga
     7501 ctcaccaata ttccttcaac atggtatccc gtgagtccta tctgtccgga tccgtccaat
     7561 aaacattgcg caacgatgat gtttttggaa aaaacaatat ccaacacatc cgaagtttcg
     7621 cacgaaaaat attcatcgcg cgtatcgtga atgcctttga ggttgaacaa cgcgatgcct
     7681 gaggagtttc gaatagaaat aatgtcaccg gttggaacct tcaaccatac ttcgccttca
     7741 ccgcgaatag tgatgttttc ttttccgtcg attactatag cctcattata tgtgccgcgt
     7801 cgtataagga tttcatcccc ggaggttgcg gagtcaatcg cggcttgaat gcttctaatg
     7861 gggccttgtg atgatactac caaaacggga cgttgtgaac ccatataagt atcgttcttc
     7921 caaaatccga tttcgcgggc gccatcgttt ttcaattttg tgccaacgcc gctaaatttt
     7981 cctaaacgca tttgccccgt ataggtggct ccgtttgcgt aatgatattc accgaagcca
     8041 tgcatccgac tatttgaata tccaccgcga taccaactct tgtctttcca gtgaaactct
     8101 ccccatcctg tacgttctcc catattgtat cctccttcgt accactcgcc atcgctccag
     8161 attgttttac cctttccgtg ttgtttgccg tttttccatt cacctgcata cgtagtgccg
     8221 gaactccata cgtatgtgcc ttcgccgttt tcacagttgc ccgacacaca accggtacgt
     8281 tgagcataca aaacggaaga caagaatagc acaaatacga ttgtggattt catacgatgt
     8341 cctttcaata cgatatattg ggtatcagga aatgcaaaga agagaatcgg gagtcaacta
     8401 taatcagtgc gtgaggtgtt taaaagtaat agatgaatcg cctttttgaa agacatattt
     8461 ttaaaatttc aaattcactt ctatatacaa aacggctttt tggagagcga gtttttatgt
     8521 aaatcgggat tttttatgag tgtcggcttg aggcgacaga ctatttttct atgtagtcgc
     8581 gtttatgtaa cgccaactaa gttattcttt tttcatttgt aaaaacatca atgagttata
     8641 aagcgcttca ttatggtatg ctaattgctt ctatctaata gttatctgta ccgtcatgtt
     8701 taatcttaat tcgacatata accatcaaac cacaggatta catctatgag aacaaaactt
     8761 ctacttctcg cttcagcatt tttgtttctg agcggctgtg ctcaactcgg cgaacttgcc
     8821 ggtaacctga tgacgcagtc cacgacggat ttgcaagcct gtggtacgac ggtgtattat
     8881 attagcaatc tttatcctgc tgccgtcaaa agcacggaaa cttcctatat gggcgaagca
     8941 tggaaagaag gccgctcggc agtaacggtg atgaatttta aaaaacatgg tatcgggatg
     9001 tataaaatag acggtatcgt ggctatggat ggcacaccga tgtcgcacgt ggcttttggt
     9061 gcatatacca aacttgtgga tagtggcgat acctcaccca aaaaaataac gatcgaaacg
     9121 aaaacgggtc aaaaagccga atttacgata aaacctacat atcccgttcg catcaaatct
     9181 attaacggta aagccgatgg cgctgaaata gacgttacgc aggatcttgt tcttgaaatg
     9241 gaaaacccca aaggttccga acgtacgatg tataaagtcg gcatgctgat ggacttgatg
     9301 ggaacgacct cttttgtaga tgtggtggtt tgccgttcag cagacaaaat cgtcataccg
     9361 caccaggctt ttgtcaacgc gcaagtcgtg gccagttcag atcaagcgat cggtacatcc
     9421 attgcgagtt ttagggaagg cgccagttgg cttcgtgtcg aacgttacga acatgatttg
     9481 tcacccaata ccggtaccgg tggatatcag gggatatccg cgagctggga tacaaagccg
     9541 gtaacggtca aaggcaaatc gccaagcaaa agcaatgttc agattaccgg cgaagagaag
     9601 ggcttttctt tcaccgccaa taaacccaat gcctattacg ggcgcgcttt atccatgggc
     9661 aaaaaatt
//
LOCUS       DAOQNQ010000107         9570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2607_length_9570_cov_6.408408, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000107 DAOQNQ010000000
VERSION     DAOQNQ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9570
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2607_length_9570_cov_6.408408"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PK754_08065"
     CDS             complement(<1..468)
                     /locus_tag="PK754_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNB09497.1"
                     /translation="MKFTVAQADLAKGLARVISVVPSKSTLPVLSNVMLKAQDGFLTL
                     TATDLDVSITTKIQVDVKQPGSITVPSKPLNDLVKSLPNIPLEVSADAQFKFFVHCDN
                     EEYRIPGESDDDYPSLPTVDEKGSLTIEAKQLSRMIGKTIFAVSNDQLRPALTG"
     gene            981..>1564
                     /locus_tag="PK754_08070"
     CDS             981..>1564
                     /locus_tag="PK754_08070"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNB09498.1"
                     /translation="MFPIRDTIPSSRFPLVTTVLIVINSLIFLYEVSLGEQLNRLINA
                     LGIIPAKYFWQGEFGRANFIERFFPFFTSMFLHGGWMHAIGNMWYLWIFGDNVEDRMG
                     RGRFLLFYLLCGLGAGLSHAYLNADSRIPTIGASGAIAGVMGAYIVLFPNSRVLTLVP
                     IFIFIQFFEIPAIFFLGFWVILQFLQGTASLMSDA"
     assembly_gap    1565..1664
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1684..5133
                     /locus_tag="PK754_08075"
     CDS             1684..5133
                     /locus_tag="PK754_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002154852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNB09499.1"
                     /translation="METEVYKPKHKIRFVTAASLFDGHDASINVMRRILQSSGAEVIH
                     LGHNRSAEDVVNTAIQEDAQAIAMSSYQGGHVEYFKYMRDLLNQKGAKHIKIFGGGGG
                     VIVPDEIKELEAYGIDRIYSPEDGRIMGLQGMINDMLRRTDFPTIDHVNGEVDALPKK
                     NPGTIAKLITYAENAVEESNGALDQLRKKLEKIKPATRIPILGITGTGGAGKSSLTDE
                     LVRRFIHDFTDKTVAIISVDPSKRKTGGALLGDRIRMNSIVNDRVYMRSLATRQSNLA
                     LSKAIQEAIEIVKAAAFDLIIVETAGIGQSDSEIVDLVDVAMYVMTSDFGAATQLEKI
                     DMIDFADIVVLNKFDKRGSLDALRDVRKQYKRSRKMFTAEDETLPIFGTIASQFNDPG
                     VNTLYVHLIAALNKKTDIGWNTCMTMTDEMSKKKFIIPPERTRYLSEITDTVRGYHRF
                     AAEQSDVARKIYQLSGTKSVFQNQDQIAGGQLRSFDMEQGVDGSVQLDFNKVSNTAEL
                     ITALDRMLDNLNIRLRPEFRKRLEEWNEFKKLYAQDKMITKVRDKEIINELSTHSLSG
                     TRIPKVSLPRYQDWGDLLSWIMKENVPGEFPYTAGVFPFKRTSEDPTRMFAGEGTPER
                     TNRRFHYLSKSQPYARLSTAFDSVTLYGEDPDYRPDIYGKIGNSGVSICTLDDMKKLY
                     AGFDLCDPNTSVSMTINGPAPMILAMFFNTAIDQQIEKHLRQNGKTDKLNKTKFDHSH
                     PHYEKAIQYDGVLFGYGTLGTTGDRLVDRETYEKIKADTLKVVRGTVQADILKEDQAQ
                     NTCIFSTEFALKMMGDMQDYYVRKQIRNHYSVSISGYHIAEAGANPITQAAFTLSNGF
                     TFVEYYLSRGMHVDDFAPNLSFFFSNGLDPEYSVIGRCARRIWSIAMKYKYKGNDRSQ
                     KLKYHIQTSGRSLHAMEIDFNDIRTTLQALMAIYDNCNSLHTNAYDEAITTPTEESVR
                     RAMAIQLIINKELGLAKNENSLQGSFIIEELTDLVEEAILGEFDRISERGGVLGAMET
                     MYQRNKIQEESLLYESLKHDGKLPIIGVNTFLNPKGVSEAQPTELIRSTKEEKEQQIV
                     NVHNFWKTNDGTAQGALDMLSQVALQNGNIFEQLMETAKSCSLGQISSTLYKVGGQYR
                     RNM"
     gene            5153..6307
                     /locus_tag="PK754_08080"
     CDS             5153..6307
                     /locus_tag="PK754_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB09500.1"
                     /translation="MLISKIILKNWRNFKKVEVDLHDRSFIVGPNASGKSNLLDAIRF
                     LRDIAKSGGGLQEAIRTRGGVSKLRCLAARSQAEIEVEAHIAENGIVLWKYAIGFSQT
                     PGGISEPKAKLKYEKVWNHNDDLIINRPDSEDEKDERLLEYTLLEQPTGNRKFRDVGD
                     FFSSIQYLHIVPQLLKDPKSYIHSTQEDYFGRDFMDRINKTNKNTRVSFLNKIEKALQ
                     IALPQFNKLELIKDHEGIPHLQARYKHWRARDAKQWEDQFSDGTLRLIGFLWSLLEGT
                     KPILLEEPELSLHSGIVSKLAEVIHKLQQKKKGKRQVILTTHSYDLLSNPGISAEETI
                     LLEPSDEGTSAKNAASNVEIKTLLTEGLSIAEIVIPKTSPKNINQIVLEF"
     gene            6310..6924
                     /locus_tag="PK754_08085"
     CDS             6310..6924
                     /locus_tag="PK754_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09501.1"
                     /translation="MNKPVIRLVVEDELHQNVLIRILKHFDDKFEIGDVYGFRGNSYI
                     KKKLHAFNEASQLSPYLVLTDLDNYACPPTLIGEWVNFTISHNMIFRIAVKEAESWLL
                     SDRKNFASYFRISAATIDLNIEAIDNPKEYLVNLARKSKNRKIREDFVPNGTALVGPN
                     YNARLADFIHNHWDYNNARRNNRSLHKLMNRLDNFVFKPAHKLK"
     gene            6928..7257
                     /locus_tag="PK754_08090"
     CDS             6928..7257
                     /locus_tag="PK754_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNB09502.1"
                     /translation="MDTIILLTISNIFMTFAWYGHLKYKDSPLWIAILISWGIAFVEY
                     CFQVPANRWGHGRFNAAELKTIQEAITLTVFCVFSVLYLKEELKWNYLVGFALMIAAV
                     FFIFKKW"
     gene            7308..8750
                     /gene="xdhA"
                     /locus_tag="PK754_08095"
     CDS             7308..8750
                     /gene="xdhA"
                     /locus_tag="PK754_08095"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HNB09503.1"
                     /translation="MKNTISFLLDGRHESITFGPDCPYTPITTVLQYLRSLPDRKGTK
                     EGCAEGDCGACTVVIGETAQAGTVRYTAVDSCLIFLPMLHGKQLLTVESLTRDEKHLH
                     PVQHALVEKHGTQCGFCTPGFVMSMFALYKNHPNPTRETINDALTGNLCRCTGYRPIV
                     EAAATVCVNERHDALSENESRHYDQLSTIIHGTIAIQTAHHRYVKPATLSEALLYLAE
                     HPEALIISGATDIALRVTKKHESLTSILDLSDVPELKDVSESGDKLILGAGLSLNQVR
                     AIVKTSHPALYDILQVFGSLQIRHLATLGGNLGSASPIGDTLPVLLAYQAKILLESVH
                     GQRRVDMDAFITGYRTTVRKADEIITAVKLPKIQPGTIVRSYKVSKRKDLDISTVSAA
                     FRLERSNDGAIRKIIMAYGGMAEKTKRAERTEAFLTGKPWTRATIESAMPLIDLEFRP
                     ISDARSGAEFRRTVARNLLLKFWSETNLMR"
     gene            9037..9366
                     /locus_tag="PK754_08100"
     CDS             9037..9366
                     /locus_tag="PK754_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HNB09504.1"
                     /translation="MRIENAPFQIFDLFQTSPVAHQGETGQALWQTVSNGNVRMRIVT
                     YSPGYLADHWCEKGHAVFVLEGAFVSELQDGRRFELTQGMSYIVADHAEPHRSYSASG
                     VKLLIVD"
BASE COUNT         2705 a         2233 c         2102 g         2430 t
ORIGIN      
        1 acccgtcaaa gccggacgca actgatcgtt actgacggcg aaaatcgttt tgccgatcat
       61 gcgggaaagt tgtttggctt cgatggtcag cgaacctttt tcgtcaacgg tcggaagcga
      121 cggataatcg tcgtccgatt caccgggaat gcggtattct tcattatcgc aatgcacgaa
      181 aaatttgaac tgtgcatcgg cggaaacttc tagaggaata ttgggcaggg atttaaccag
      241 atcgttgagg ggtttggacg gtaccgtgat gcttccgggt tgtttgacat ccacttgaat
      301 tttggtggtg atcgaaacat ccaaatccgt agcggttaac gtaaggaaac catcttgcgc
      361 tttgagcatg acgttgctca gcacgggcaa cgtggatttt gagggaacga cgctgataac
      421 acgcgccaat cctttggcca gatcggcctg agcaaccgta aacttcatat cctgcaactc
      481 ctgtggctaa ataacaaata tattctattc aaaaaattat agtgccaaac gacgggtttt
      541 atttcaccgt gaaattcaac aaaatcaagg gttgacgggt atttttaaaa ctgaaaatca
      601 tagtgtttta ttgtcaaaaa ttcaagcaaa atattatgat tatcgccgcc aaaaacaccc
      661 tatccgtaaa gttccatgcg cgtttgtatg acgtacatgc ccttgttttt tataaaaaat
      721 agttccgttt tatcggcgaa tgttattaat ttgttttgcc tgtttgtgta aaataatttt
      781 tgcgcgaagc gcgataatac aaaccgttta ttttatttgt tcacgttttc gttttagtgt
      841 tacaggcgcc acgcattcaa acaagggaag gtgtttttga acaatacgat ccataactat
      901 taaacaccat gtatcatatt ttcggaacga taacccaaaa cacgtacaca aaaaatacgg
      961 acaagcatac acattaaact atgtttccca ttcgcgacac catcccttct tcccggtttc
     1021 cgctggtcac gactgtattg atcgtgatca atagcctgat ctttctgtat gaagtatcgt
     1081 taggcgagca actcaaccga ttgatcaacg cgctaggcat catacctgca aaatattttt
     1141 ggcaaggtga gtttggtcgc gccaatttca tcgaacgctt ttttccgttt ttcacatcca
     1201 tgtttttgca tggcggttgg atgcatgcca tcggtaatat gtggtatctg tggattttcg
     1261 gcgataatgt ggaagatcga atgggtcgcg ggcgtttttt gctgttttat cttttgtgcg
     1321 gactcggtgc cgggctttcg catgcgtacc tcaatgccga ctcgcgcata cctacgatcg
     1381 gcgccagcgg tgccatagcc ggcgtgatgg gcgcttatat cgtattattt ccgaattcgc
     1441 gtgtacttac actggtgccg attttcattt ttattcagtt ttttgaaatt cccgccattt
     1501 tttttctcgg tttctgggtt attttacaat ttttacaagg aacggcttcg ctcatgtccg
     1561 atgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntatttt ttaaggatac
     1681 agcatggaaa cggaagttta caaacccaaa cataaaatcc gatttgttac tgcggccagt
     1741 ctttttgacg ggcacgacgc gagcatcaat gtcatgcggc gtatcctgca atcgtcgggc
     1801 gcggaagtca ttcacctcgg acacaatcgc agcgccgaag acgtagtcaa tacggccata
     1861 caggaagacg cgcaagccat cgcgatgagt tcgtatcagg gtggccacgt cgaatacttc
     1921 aaatacatgc gcgatctgct caatcaaaaa ggcgccaaac atatcaaaat tttcggcggc
     1981 ggcggcggtg tgatcgttcc cgacgaaatc aaagaactcg aagcgtacgg cattgaccgg
     2041 atatactccc ccgaagacgg tcgcatcatg gggctgcaag gcatgatcaa cgatatgctg
     2101 cggcgtacgg attttccgac gatcgatcac gtcaacggcg aagtggacgc tttacctaaa
     2161 aaaaatccgg gcaccatcgc caaactgatc acgtatgccg aaaacgccgt cgaagaaagt
     2221 aacggcgcgc tcgaccagct tcgcaaaaaa ttagaaaaaa tcaaaccggc tacacgcata
     2281 ccgattctcg gcatcacagg taccggcggc gccggcaaaa gttcactcac agacgaactg
     2341 gtacgccgtt ttattcatga ctttacggat aaaaccgtgg ccatcatttc cgttgatccg
     2401 tccaaacgta agaccggcgg cgctctgctg ggtgatcgta ttcgcatgaa cagcatcgtc
     2461 aatgaccgcg tgtatatgcg ttcgctcgcc acgcgtcaat ccaatctggc actgagtaaa
     2521 gccattcagg aagctatcga aatcgtcaaa gccgcagcgt ttgatctcat catcgtcgaa
     2581 accgcaggta tcggacagtc ggattcggaa atcgtggacc tcgtggatgt agcaatgtat
     2641 gtgatgacca gcgatttcgg cgccgcgacc cagctcgaaa aaatagatat gattgatttt
     2701 gccgatatcg tcgtgctcaa caaatttgac aaacgcggtt cgcttgatgc gctgcgcgac
     2761 gtacgcaaac agtacaagcg ctcccgcaag atgtttaccg ccgaggacga aacgctgcct
     2821 attttcggca ccatcgccag ccaatttaac gatcccggcg tcaatacgct gtatgtgcat
     2881 ctcatcgccg cgcttaataa aaaaaccgac atcggctgga atacctgcat gacgatgacg
     2941 gatgagatga gtaaaaagaa atttatcatc ccgcccgaac ggacgcggta cctcagcgaa
     3001 atcaccgaca cggtccgggg ttatcaccgt ttcgcggcag aacaatccga cgtagcgcgt
     3061 aaaatctacc aactcagcgg caccaagtcg gtatttcaaa atcaggatca aatcgccggc
     3121 ggacagttgc gctcgtttga tatggagcaa ggcgtggacg gatcggtaca acttgatttt
     3181 aataaagtat ccaataccgc cgaactcatc accgcgcttg atcgcatgct ggataatctt
     3241 aatatccggt tgcgtccgga atttcgcaaa cgcctcgaag agtggaatga atttaaaaaa
     3301 ctgtatgcgc aggacaaaat gatcaccaaa gtgcgcgata aggagatcat caacgagctt
     3361 tcgacgcatt cgctttccgg tacgcgcatt cccaaagttt cgctccctcg gtatcaggat
     3421 tggggtgatc tgctgtcatg gatcatgaaa gaaaatgtac ccggcgaatt cccctatacg
     3481 gccggcgttt tccctttcaa acgcaccagt gaagatccga cgcgtatgtt tgccggcgaa
     3541 ggaacacccg aacgcaccaa ccgtcgtttc cattatttgt ccaaatctca gccttatgcg
     3601 cgtttatcca cagcatttga ttccgtcacg ctctacggcg aagaccccga ttaccgtccg
     3661 gacatttacg gcaaaatcgg aaattccggc gtttcgatct gtacgctcga cgatatgaaa
     3721 aaactgtatg ccggatttga tctctgcgat cccaatacgt ccgtatccat gacgatcaac
     3781 ggccccgcgc cgatgattct cgccatgttc tttaataccg ccattgatca acagatcgaa
     3841 aaacatctac ggcaaaacgg taaaacggat aaacttaaca agaccaaatt tgatcacagc
     3901 catccgcatt atgaaaaagc catccaatac gacggtgtgc ttttcggata cggtacgctc
     3961 ggcacgacgg gagaccgctt ggtagatcga gaaacctatg aaaaaatcaa agccgatacg
     4021 ctcaaggtcg tgcgcggtac ggtgcaggcg gatattctca aagaagatca ggcacagaac
     4081 acatgtatct tttcgacgga gtttgccctc aaaatgatgg gtgatatgca ggattattac
     4141 gtacgtaaac aaattcgcaa tcactactcc gtcagcattt ccggatacca catcgccgaa
     4201 gccggcgcta acccgatcac gcaagccgct tttacattgt ccaacggatt cacttttgtg
     4261 gaatactatc tctcgcgcgg aatgcatgtg gacgactttg cgccgaatct ttcgttcttt
     4321 ttcagcaacg ggcttgatcc ggaatattcc gttatcggtc gttgtgcacg gcgtatctgg
     4381 tctatcgcca tgaagtacaa atacaaaggc aatgaccgtt cgcaaaaact caaataccac
     4441 atccagacca gcggtcgcag tttgcatgcg atggagattg atttcaacga cattcgcacc
     4501 acgttgcaag cactgatggc gatctacgat aactgcaatt cgctccacac caatgcctac
     4561 gacgaagcga tcacgacgcc gacggaagaa tccgtacgac gtgccatggc gattcagctc
     4621 atcatcaata aagaactcgg tttagcgaag aatgaaaatt cactccaggg ctctttcatc
     4681 atcgaagagt tgaccgatct cgtggaagag gcgattttgg gcgaatttga ccgcatctcc
     4741 gaacgcggcg gtgtattggg cgcgatggaa acgatgtatc agcgcaataa aatacaggaa
     4801 gaatctttgc tgtacgaaag cctcaaacac gacggcaaac ttccgatcat cggcgtcaat
     4861 acgtttctca atcccaaagg cgtaagcgaa gcgcaaccga cggaactcat ccgctcgacc
     4921 aaagaagaaa aagaacagca aatcgtcaac gtgcataatt tttggaaaac caacgatggc
     4981 acggctcaag gcgccttgga catgctgtcc caagtggctc tgcaaaacgg caatattttc
     5041 gaacaactga tggaaaccgc caaatcctgc tccttgggac aaatcagttc cacgctgtat
     5101 aaggtcggcg gtcaataccg caggaatatg taaaacaaac aacaagaaaa acatgctgat
     5161 ttccaaaatt attttaaaaa actggcgcaa ctttaagaaa gttgaagtcg atctacatga
     5221 tagatcgttt attgtaggac ccaatgcatc cggaaaatcg aatttattgg atgcgatccg
     5281 atttttacgg gacatcgcta aaagcggagg agggcttcaa gaagcaatac gaaccagagg
     5341 cggcgtttca aaattacgat gccttgctgc tagatctcaa gctgaaattg aagttgaagc
     5401 ccacattgca gaaaatggaa tagttttatg gaaatatgcg attggtttta gtcaaactcc
     5461 gggcggcata tcagaaccaa aagctaaatt aaaatatgaa aaagtttgga atcacaatga
     5521 tgatctgata ataaacagac ccgatagcga agatgaaaaa gatgaaagac ttttagaata
     5581 tacgcttttg gaacaaccaa caggcaatcg taaatttaga gatgtcggag attttttttc
     5641 ttctattcaa tacttacata ttgtgcctca actactaaaa gatccaaaat cttacataca
     5701 ctcaacgcaa gaggattact tcggtcgtga ttttatggat agaataaata aaacgaataa
     5761 gaatacacgt gtatcatttc taaataaaat tgaaaaggct cttcaaattg cattgcctca
     5821 atttaacaaa ttagaattaa taaaggatca cgaaggtatc cctcatcttc aagcgagata
     5881 caaacattgg cgagcccgag atgcaaagca atgggaagat caattttcag acggcacatt
     5941 acgtttaatt ggttttttat ggtcattgtt agaaggtaca aagccaatac tacttgaaga
     6001 acccgaactg tcgttacatt cgggtatagt ttctaagtta gcagaagtta ttcataagct
     6061 tcaacagaag aaaaaaggga aaaggcaggt aattttaacg actcacagtt atgacttgtt
     6121 atccaatccc ggaattagcg ctgaagaaac cattttacta gaaccgtctg atgaaggtac
     6181 ttccgccaaa aacgcagcca gcaatgttga aatcaaaact ctattaacag aagggttgag
     6241 cattgcggaa attgtaattc ctaaaacttc accgaaaaat attaatcaga ttgtattaga
     6301 gttttgacga tgaacaaacc agttattcgt ttggttgttg aggatgaatt acatcaaaat
     6361 gttttaatta gaatcttaaa acattttgat gacaaatttg aaattggcga tgtttatggc
     6421 ttcagaggga acagttatat taaaaagaaa ttacatgctt tcaatgaagc atcacaatta
     6481 agtccttatt tagtcctgac tgatctagac aactatgcgt gcccaccgac cctaataggt
     6541 gaatgggtta attttacaat atcgcacaac atgattttta gaatcgcagt aaaagaagcc
     6601 gaaagttggt tattaagcga tcggaaaaat tttgcaagct attttagaat atctgccgct
     6661 acgattgatt tgaatataga agccatagac aatcccaagg agtatttggt taacttagct
     6721 cgaaaatcaa aaaacagaaa aataagagag gattttgttc cgaacggaac cgcactagtc
     6781 ggacctaatt acaatgcgcg acttgctgac ttcatccata atcattggga ttacaataac
     6841 gctagaagaa acaaccggag tcttcataaa ctaatgaacc gcttagataa ctttgttttt
     6901 aagccggctc acaaattaaa ataatgcatg gacacaatca tactcctgac gatttccaat
     6961 atctttatga cttttgcatg gtacggtcat ctgaagtata aagattcgcc gctgtggatt
     7021 gccattctca taagttgggg tatcgcgttt gtggaatatt gttttcaagt gccggccaat
     7081 cgctgggggc acggacgatt taatgccgca gaactcaaaa ccattcagga agccatcacg
     7141 ctcaccgtat tttgtgtgtt ttccgtattg tacctcaaag aagaattaaa atggaattac
     7201 ctcgtgggtt tcgctctgat gatcgccgct gtttttttca tattcaaaaa gtggtagtcc
     7261 cgtcacgcgt tatcccctat cacagctgat atcctgaaag ttggctcatg aaaaacacga
     7321 tttccttttt actcgacggt cggcacgaat ctattacgtt cggaccggac tgtccttata
     7381 caccgatcac gacggtactg cagtacctgc gcagtttgcc ggatcgcaaa ggcaccaaag
     7441 aaggttgcgc ggaaggcgac tgcggcgcgt gtacggtggt catcggcgaa accgcacaag
     7501 ccggcacggt acgctacacc gccgtggaca gttgtttgat atttttaccg atgctccatg
     7561 gtaaacagct tctgaccgta gaaagcctca cacgcgatga aaaacatctt caccccgtgc
     7621 aacatgcctt ggtcgaaaaa cacggaacgc aatgcggttt ttgtacgccg ggtttcgtca
     7681 tgtccatgtt cgcactgtat aaaaatcatc ccaaccccac gcgcgaaacc atcaacgatg
     7741 cgctgaccgg taatctctgc cgctgcacgg gttaccgtcc gatcgtcgaa gcggcggcca
     7801 cagtctgcgt gaatgaaagg cacgacgcgc tgagcgaaaa cgaatcgcgc cactacgatc
     7861 agctttcgac gatcatacac gggacgattg cgattcagac agcgcaccat cgctatgtca
     7921 aacccgccac actcagtgaa gccttacttt accttgctga acacccggaa gccttgatca
     7981 tcagcggcgc caccgacatc gcactgcgtg tgaccaaaaa gcacgaatcg cttacttcta
     8041 ttctcgacct ctccgatgta ccggaactca aagatgtatc ggaatcgggc gacaaactga
     8101 tcctcggtgc cggactttcg ctcaaccaag tgcgcgcaat cgtaaaaact tcacaccccg
     8161 cgctgtatga catactccaa gttttcggtt cgctgcaaat acgtcatctc gccacgctcg
     8221 gcggaaatct cggaagcgct tcgcccatcg gcgatacgtt gcccgtatta ttggcgtatc
     8281 aagccaaaat tttattagaa agcgtacacg gtcaacgccg cgtggatatg gatgcattta
     8341 tcaccggtta ccgtacgacc gttcgtaaag ccgatgaaat catcacggct gtaaaacttc
     8401 ccaaaatcca acccggtacg atcgtacgtt catacaaagt atcgaaacgc aaagaccttg
     8461 acatatccac cgtcagcgcg gcatttcggc ttgagcgaag caacgacggc gccatccgaa
     8521 aaatcattat ggcttatggc ggcatggctg aaaaaaccaa acgtgccgaa cgcaccgaag
     8581 ctttcctcac cggcaaaccc tggacacgcg ctaccatcga atccgcgatg ccgctcattg
     8641 atttggaatt tcggcccatt tccgatgcac gttccggcgc tgaatttaga cgcacggtgg
     8701 cgcgcaatct ccttttgaaa ttttggagtg agacgaattt aatgcgttaa cagcctcgta
     8761 attgtgtact tgcgacgtac ggttgattcg gcgactatct ttgagaggta tgtgcactta
     8821 ctatatcaaa aaatttcttt ccatatctct ttgcggcttt gcgagggtaa attcattctc
     8881 gccaagacgc gaagactcaa aaattatcag aaccttcata attgaattta aatgcgctaa
     8941 aacgacgagc gcttgattta ctgcttatat tttgacaatc atgaggccgc tattaattcg
     9001 ttttttcact actctatcgg ttaaggagtt ttttctatgc gtattgaaaa cgcaccattt
     9061 cagatttttg atttattcca aacatcgccg gtggcgcatc aaggagaaac cggtcaagcg
     9121 ctgtggcaaa ctgtttcgaa cggtaatgta cgcatgcgta tcgtcacgta ttcacccggt
     9181 tatctcgccg atcattggtg tgaaaaaggc catgccgtat ttgtgttgga aggcgccttt
     9241 gtatccgaac tgcaagacgg gcgtcgattt gaactcacgc aaggcatgag ttatatcgtg
     9301 gccgatcatg ccgaaccgca tcgctcgtat tccgcatcgg gcgtcaaact tctcattgtt
     9361 gattaaatta tactctacta ataattaaaa tttggagatc atttgaagca cagctttccg
     9421 agtgtcttat tttttttggg attattcatc gcgtacgttc cctgtacgca tgctcagaat
     9481 gaaacttttg aaatggttat acaaagttcg cataccggtg aagtcgcgtc cgtggatttt
     9541 gctccggacg gcaaaacctt catcaccgcc
//
LOCUS       DAOQNQ010000108         9521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2631_length_9521_cov_6.591380, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000108 DAOQNQ010000000
VERSION     DAOQNQ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9521
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2631_length_9521_cov_6.591380"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PK754_08105"
     CDS             <1..723
                     /locus_tag="PK754_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09505.1"
                     /translation="DYIKDHSIAIGLIPTATWSLSEKYWGYRAIEKTITDYRGLGGAS
                     DIGIVAKGKFLDDGMIGYTLSVVNGNGQKPENNKSRKYIGSLSVKPIKSVITEIYADY
                     EGSITEPKKYGKVDSRNTLKALVAYQSDIATTGLEVVQQTQKRTDTTDVKPFGFSVFA
                     SAPVMKEKLNVFARYDSFDPNTEITKAGFNEYFFTAGVDYMPIKNVHFMPNIWINGFS
                     AKKGSSRDADVVARLTFFYVYK"
     gene            857..1186
                     /locus_tag="PK754_08110"
     CDS             857..1186
                     /locus_tag="PK754_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNB09506.1"
                     /translation="MKTNKKKQIPKNYQNFVKDYPRVAKAYEELGDAVHHAGPLDTKT
                     RALIKLAISAASRQEGAVHSHVRKALTAGASPEEMRHVIMLTLPTIGFPGMMAAMSWV
                     NDIISSK"
     gene            1291..1545
                     /locus_tag="PK754_08115"
     CDS             1291..1545
                     /locus_tag="PK754_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNB09507.1"
                     /translation="MMHIQIAGPGCINCTKLEDMCREAIHAYHVDAQIERITERQDYS
                     RLGVFATPGLLINGHIISTGRMPAYLTVEKWIKSAEDWSS"
     gene            1773..2342
                     /gene="msrA"
                     /locus_tag="PK754_08120"
     CDS             1773..2342
                     /gene="msrA"
                     /locus_tag="PK754_08120"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009053686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNB09508.1"
                     /translation="MRKEPMITNQAAVDTATFGAGCFWCVEAVFQRTKGVLSVKSGYS
                     GGHVDNPTYQQVCNGNTGHAEVIQLTFDPKTVSFVELLEVFFGTHDPTTLNRQGADEG
                     TQYRSAIFYHSDDQRRIAEDVKQKLDASGAFKNPIVTEISAFNKFYPAEDYHQNYFND
                     NGNAPYCAYVIKPKLEKFRKVFKDKLKAD"
     gene            complement(2504..3562)
                     /locus_tag="PK754_08125"
     CDS             complement(2504..3562)
                     /locus_tag="PK754_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB09509.1"
                     /translation="MLISDRGMMAWDTRRNRHWIKQAVKRDVLIGDIALSISARYKMS
                     SQTATISGPGGFNKKGGLTYTVSSASAGTSLQYTWYLKYDFNSVWQNIGSGTSKFINI
                     SALSNAYNFNLRVYMRNLTDTIGVWSNEYRVTCLSCGGGYNDPNQRRDMADAGAEEDS
                     VTHGEWWPVNADTLTMHHKRRILMPIRDAIRMMNHRFRWKKNDMFTIGTNDDLIEDEE
                     SAYTEKNEPNESSIITEHIAQNDKISKKQENGDPAMPFDQTPKRFALYEAYPNPFNPS
                     TTLAFDVIEKSEVSLIIYNTLGQTVRRLITETRSSGHHRVLWDGKNDSGVLVPSGVYI
                     YQLRAGNNIQSRKMIFMK"
     gene            complement(3483..4352)
                     /locus_tag="PK754_08130"
     CDS             complement(3483..4352)
                     /locus_tag="PK754_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09510.1"
                     /translation="MRLVITLFIMLLPVSPRVWAHGLELECFYKQNGTRVFPANYTIQ
                     IKPAANTTIYGWDNNFAAIPQGDPAYYMFEANHIKADFIYPDGLANSHNYTTYYGLYE
                     VTFLEIVNDEKIPRGTVYFDNRDANYHGLSSSPYPTRDFYITYDLNSGYAQVSTREGI
                     GTPCDLIVDGQPVNNNGTSIIYTTWCFFGIVPNQTPFKNFALDVTGNNTNDVYSIKVG
                     SLSCGRTGNTSLIEGLGFLAPNTRTVELPYRIAQVDADGEPTGGTYAYFRSWDDGVGY
                     APQSPLDQASGKT"
     gene            complement(4372..5625)
                     /locus_tag="PK754_08135"
     CDS             complement(4372..5625)
                     /locus_tag="PK754_08135"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNB09511.1"
                     /translation="MIRKLAVSLGIVMMISCDTSHHGRDRCEEGMLSASDAVWTHSEG
                     DLQNTKRATGMRIKGCQAGPITKPVVQWAFELGGPGTAAAPVIGDDGTIYITGEYPGE
                     PLGGGWRNAGLMAINPNGTLKWFFHDSLDIGTGLAVLYQKSAAIDQSGILYYSGYGGV
                     IYAMSPDDGKVLWSVRRAGGANPVIDERRNIYTANDTVFCFKYDGTLRWTFAEDTSLG
                     QCEEIALGEDMIFCSFFRKGVLALDYKGNKKWFHPAEMTSLRAGIIVDESDNIYFKVN
                     SGNIRSVDRYGQVRWYGSAGGGFTALALRGEYLYFGSLSSLYKLDKATGTQITYIGGA
                     MGHYYLESSSPLVDDAGNVFIATSTDYVLGAQNDGQELWHTTLDDLSNLAECYGPLAL
                     GHDGTIYVPVFNYISGKVQHLYAIK"
     assembly_gap    5863..5962
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6414..9368)
                     /locus_tag="PK754_08140"
     CDS             complement(6414..9368)
                     /locus_tag="PK754_08140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB09512.1"
                     /translation="MRLGSQRLSRTSRTWSATEPPPVTLVVDDALLLDVLAGVADPEL
                     QQAADRGEVHTTGCWYYRLSRALHSEPMIGAYLKLAGTSLGAVSDREGRFRISGIASG
                     SYTLKVSYLGYTTYEKKFTVAAGDHTKMEILLKAGSIELSGSEVYGMREGQAKALNLQ
                     KNADNQQVVTSSEIIKLFPDPNISEALQRMPGVAVLRDQGEGRYVFLRGLEPKYSSVK
                     INGQPVPSPEGDIRQVSLDVISSSMVSRIEVSKTLTPDMDADAVAGAINLVTKNALDF
                     DQQMWQVSAGTGRNMLEGKPLANFDITYGNRFGEEKRLGVFVNAMGYYTHRNSDDMER
                     VWGERAFNGQNHYFMNEFELRDYNAIRKRLSFNAAADYRFSETSDIHFGAVHNYFRDD
                     EIRRRLRIKLSEGTAPVRARRFKFVSDSSVTMDSAVVERETKDWALTRSLTSFQMSGH
                     AQWADMDWTYSAALSRATEAIPDRFDFTTRQTDVNLKYDMTNRQFPKFAFLSGDPYDP
                     ALFKLKNFEVQEYDITEWDRIAELNIRKSLRILSNGDYVQSGAKVRSKAKERNDEHNR
                     ATIANPNAPPFMNFFAESDEDINFLYSRYRFGTSADPSLIRRWADTANLINSANYFAD
                     ESFITNYDADEMIIAVYGMTKLNVNRVDVVAGVRMEKTYIDYNGVKVVQNGKAIRSTQ
                     LGYPDTLHFAGYPIDTVSLKGHYEYYQFFPSLNLKYNITDRFSVRAAIGRAIFRADYF
                     DLIPYEIFDYQNFTISRGNTYLRPTVSWNYDISADYYLKDVGIIAGGFYYKVLNDFIY
                     RRQYIDTTLNEGSPWGVNQWRVNQKQNGKVARLFGAEFNWQQTFTFLPPNFDGLGIYL
                     NYAYTHSQAYYPQRPGNKNTLPGQPNHVGNFALTYEKKGFAARVAWNYSGPYVLTVSD
                     SVDTDFDTYVDRHLQLDISASQKLTKHIRVYLELNNLTNEPYREYVGSRKRLRQQEYY
                     SWWGKLGAEYTF"
     gene            complement(9313..>9521)
                     /locus_tag="PK754_08145"
     CDS             complement(9313..>9521)
                     /locus_tag="PK754_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09513.1"
                     /translation="LVGEVGERDAEARRAIAEEHRATPPPSGQLGSWLTDDEREAVRA
                     AVADGAYAAGVAAVVADEPDLVSD"
BASE COUNT         2614 a         2284 c         1985 g         2538 t
ORIGIN      
        1 gattatatta aagatcactc catcgcgatc ggtttgatcc caacagcaac atggtcgtta
       61 tctgaaaaat attggggtta ccgagcaatc gaaaaaacta ttacagatta tcgtggattg
      121 ggtggcgcat ccgatattgg tattgtagcg aaaggaaaat ttcttgatga tggaatgatc
      181 ggctatactt tatctgtcgt caacggaaac ggtcaaaaac cggaaaacaa taaatctaga
      241 aagtatatcg gatcactgag tgttaaaccg atcaaatcgg ttataacaga aatttatgct
      301 gactatgaag gttccatcac ggagcccaaa aaatatggca aagtggatag ccgtaacact
      361 ctgaaagcgc ttgttgcata tcagagcgat attgccacca ccggtttgga agttgttcag
      421 caaactcaga aacggacgga cacgacggac gtaaaaccgt ttgggttctc cgtatttgca
      481 tctgctccgg tgatgaaaga aaaactcaat gtgtttgcac gttacgattc atttgacccc
      541 aatacggaaa tcaccaaagc cggattcaat gaatatttct ttaccgcagg tgttgactat
      601 atgccgatca aaaatgttca cttcatgccc aatatttgga tcaacggttt ctcggccaaa
      661 aaaggcagtt cccgtgacgc cgatgtggta gcgcgtttga cattttttta tgtgtataaa
      721 tagtttttta taacgccctt atgaagggat gtcccggatt gcgaaatcaa tttgggacat
      781 cccttttttt attaattttt tttgagtaaa ctcatggcgt gaataatctg tgatccatat
      841 ttgaaagtaa gtacccatga aaacgaataa aaaaaagcag atcccgaaaa attatcagaa
      901 ttttgtcaaa gactatccgc gtgtagctaa agcgtatgag gaacttggcg atgccgtgca
      961 tcatgccgga cctctggata ccaaaacccg tgcgctgatc aaattggcta tttccgcagc
     1021 cagccgtcag gaaggcgccg tacattcgca tgtgcgcaaa gccctaacag ccggcgcttc
     1081 accggaggaa atgcgtcacg taatcatgct tacgcttccc accatcggat ttcccggcat
     1141 gatggcggcg atgagctggg tcaacgacat catcagttca aaatagaatt taaaacacct
     1201 gatttcggtc aatcgccatc ctgactctcc gcattgccat cacgatgcta ttcgtcgtat
     1261 tattttttaa tagacgaagg aggtctcatc atgatgcaca ttcagattgc cggcccgggt
     1321 tgtatcaact gtaccaaact cgaagatatg tgccgtgaag cgatacatgc ttatcacgtg
     1381 gatgctcaga tcgaacgcat taccgaaaga caggactaca gccgccttgg cgtttttgcg
     1441 acgccgggct tgctcatcaa tgggcacatc atcagcaccg gtcgcatgcc ggcttatctt
     1501 acagtcgaaa aatggatcaa atctgcggaa gattggtctt cttaaacgat tggataaata
     1561 attttacttc aaggtttgtg acgacgatca caaacctttt ttattttgta ttattttatt
     1621 gtcataaggg aatagatgac gattgtcacc gttgtattac acggcattaa catgaaagga
     1681 attttatgag tttttactct ttattgagta tgttatgtat catcggttgc agtaaagaac
     1741 atgataattc aaccgaaatt ttacgttcaa ccatgaggaa ggaacccatg ataacaaatc
     1801 aagcggcggt agataccgca acgtttggcg ccggctgctt ttggtgtgtg gaagccgtgt
     1861 ttcagcgaac caaaggggta ttgtctgtca aatccggtta cagcggcggt cacgtggaca
     1921 acccgacata tcaacaagtc tgcaacggca ataccggcca tgcggaagtc attcaattga
     1981 catttgatcc taaaaccgtt tcgtttgtcg aattgcttga agtgtttttt ggcactcacg
     2041 atccgaccac actcaaccgt caaggtgccg atgagggaac acagtatcgc tccgctattt
     2101 tttatcatag tgatgatcag cgccgcatcg ccgaggacgt caaacaaaaa ttagatgcat
     2161 ccggtgcatt caaaaacccc attgtcaccg aaatttcggc gtttaataaa ttttatcccg
     2221 ccgaagatta ccatcaaaat tatttcaatg ataacggtaa tgcgccgtac tgtgcgtacg
     2281 tgatcaaacc aaaattagaa aaattcagga aggtttttaa agataagttg aaagcggact
     2341 gaatctaaat aagaaaacta tcacctataa agactacaaa ccatacgatt tgccataaca
     2401 aaataaacgc tcctgaatag gagcgtttat ttttatacaa caccttgtga cgaagtgcat
     2461 ccgtatcatg cgaaaaattc ggataactat aaaaaacaaa ctctcacttc ataaaaatca
     2521 ttttgcggct ttgaatgtta ttaccggcgc gtagctggta aatatatacg ccgctgggta
     2581 cgagcacgcc gctgtcattt ttaccatccc agagtacgcg atgatgaccg gaagaacggg
     2641 tttccgttat caaccgcctt accgtttgcc ccaacgtgtt atatatgatc agcgaaacct
     2701 ccgatttttc gatcacatca aacgcaagcg tcgtacttgg attaaaagga ttgggatatg
     2761 cttcgtatag cgcaaaacgt ttcggcgtct gatcaaacgg catcgccgga tccccatttt
     2821 cctgcttttt actgatttta tcgttttgag cgatatgctc tgttatgata gatgattcat
     2881 tcggttcgtt tttttccgta taagcgcttt cttcatcctc gatcagatca tcgttggtac
     2941 ctatcgtaaa catgtcattt ttcttccacc gaaagcggtg attcatcata cgaatagcgt
     3001 cgcgtatagg catcaatatt cttcttttat gatgcatagt gagggtatcg gcattgaccg
     3061 gccaccattc tccatgcgtg acactatctt cctcagcccc ggcatcggcc atgtcccgcc
     3121 tttgattggg atcattataa ccgccgccac acgacaaaca agtgacgcga tattcattgg
     3181 accagacccc tatggtatcg gtcagattac gcatatacac acggagatta aaattatacg
     3241 cgttgctcag tgcactgatg ttaataaatt ttgaagtccc gcttccgata ttttgccata
     3301 cggaattaaa atcgtatttt aaataccacg tatattgcag ggatgtcccg gctgatgccg
     3361 aagagacggt atatgtaagt cccccttttt tattaaatcc acccgggccg ctgatcgtgg
     3421 ccgtctgaga gctcatctta taccgcgcac ttatgcttag tgctatatcc ccgatcaaaa
     3481 catcacgttt taccgcttgc ttgatccaat ggcgattgcg gcgcgtatcc cacgccatca
     3541 tcccacgatc tgaaataagc atacgtaccg ccggtaggct ctccgtcagc atccacctgt
     3601 gcaatccggt aagggagctc gacggtccgc gtattgggcg ccaaaaagcc aagtccttcg
     3661 ataagagaag tattaccggt acgcccacaa ctgagcgaac cgactttgat cgaatacaca
     3721 tcgttggtgt tattacccgt aacatccaat gcaaaatttt tgaaaggggt ttgattgggt
     3781 acaattccga agaaacacca tgtcgtatag attatggatg tgccgttatt attgaccggc
     3841 tgaccgtcaa ctatcaggtc acacggagtg ccaataccct ccctcgtaga gacctgtgca
     3901 taaccgctgt taagatcata cgtgatatag aagtcgcgcg taggataagg cgaagagctc
     3961 aaaccgtgat aattagcatc ccggttatca aaataaactg taccacgagg tattttttca
     4021 tcattcacaa tttccaaaaa tgtcacctca tacaaaccgt aataagtggt gtagttatga
     4081 ctgtttgcaa ggccatccgg atagataaaa tccgctttga tatgattggc ttcaaacata
     4141 taatacgcag gatcgccctg agggatcgcc gcaaaattat tatcccatcc atagatcgtc
     4201 gtattcgcag ccggtttaat ctgaatagta tagttagccg gaaacacacg ggttccgttt
     4261 tgcttgtaga agcattctaa ctctaatcca tgagcccata cccgcgggct aaccggcaat
     4321 agcataataa ataacgtaat aaccaatctc ataacaaact cctttccctt tttatttgat
     4381 cgcatagaga tgttgtactt tacctgaaat gtaattgaat acggggacat atatcgtgcc
     4441 gtcatgtccc aaagccaatg ggccatagca ctcggcgagg ttactgagat catccagtgt
     4501 ggtatgccac agttcctggc cgtcgttttg agcccccaaa acatagtcgg ttgatgtagc
     4561 gataaacaca ttaccggcgt catccaccag cggagaagac gattctaagt agtaatggcc
     4621 catggcgccg ccgatgtatg tgatttgtgt accggttgct ttatctaatt tataaagact
     4681 agataacgag ccaaaataca gatactctcc ccgcaaggct aaggccgtga atcctcctcc
     4741 ggccgatcca taccatcgca cctgaccata gcgatccact gaacgaatat tgccgctatt
     4801 gaccttaaaa taaatgttat ccgattcgtc cacgatgata cctgcgcgta aactggtcat
     4861 ctccgcaggg tgaaaccact tcttatttcc cttatagtcc agcgccagca cccccttacg
     4921 aaaaaaagaa caaaatatca tatcctctcc cagtgcgatc tcctcacact gccccagact
     4981 cgtgtcttcg gcaaacgtcc atcgcagggt accgtcgtac ttaaaacaga acaccgtatc
     5041 attggccgta tagatattcc ttcgctcatc aataaccggg ttggctcctc cagctctacg
     5101 cacactccat agcaccttac catcatcggg tgacatggcg taaataacgc ccccataccc
     5161 tgagtaataa agaatacctg attgatcaat cgctgcgctt ttctgataca agacagccaa
     5221 accggttcca atatcaagcg agtcatgaaa aaaccacttg agtgtaccat tgggattgat
     5281 ggccatcagt ccggcattgc gccagccgcc ccccagcggc tctccggggt attctccggt
     5341 gatatagatc gtaccgtcat cgccgatcac cggcgcagcg gccgtccccg ggccgcccaa
     5401 ttcaaacgcc cactgtacaa ccggcttcgt gatcgggccc gcctggcatc ctttgatgcg
     5461 catacccgtt gcacgtttcg tattctgcaa atcgccctcc gaatgcgtcc ataccgcatc
     5521 cgatgcggac aacataccct cttcacaacg atcacgaccg tgatgcgatg tatcacagga
     5581 aatcatcatc acgatcccca gcgatacagc cagctttcgt atcatgacgg tattttttta
     5641 tttgttatgc gcatggtgtg actctttggg ttttgtcttc atctatatat gatagtaccg
     5701 taatcatcaa aattattaca attttttttg gggttgttgc ctcataacaa actccttttc
     5761 attttttatt tgatcgcata cagatgctgt actttacctg aaatgtcatt gaatacgggg
     5821 acatatatcg tgccgtcatg tcccaaagcc aatgggccat agnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nncctcttca caacgatcac gaccgtgatg cgatgtatca
     6001 caggaaatca tcatcactat ccctagcgat acagccagct ttcgtatcat gatggtattc
     6061 tttttatttg ttatgcgcat ggtgtgattc tttgggtttt gtcttcatct gtatatgata
     6121 gtaccgtaat catcaaaatt atcacaattt tttttggggt tgctcagatt tatgcagata
     6181 aagacaaaat cactaagtaa taacgaatcg ctacccgaaa gttttaaaaa acactttaac
     6241 ccttgggacg acagataaga tcacctaaat gcaaaatatt aagcccctta gaggcagaat
     6301 tgatgattct gtgcgtcaaa tgaacagcac tgtagaaaat tgggtttatc aaagcacacg
     6361 ataaagtagc tcaacccaac gtcgtcaggc tgatctccgt ctagagatag aaattaaaat
     6421 gtatattccg cacccaattt accccaccac gaataatatt cttgctgacg taaccgctta
     6481 cgggaaccga cgtactcacg atacggttca ttggtcaggt tgttgagctc aaggtaaaca
     6541 cgaatatgtt tggtcaattt ctgggaagcg gaaatatcca actgcaaatg ccgatccaca
     6601 tacgtatcaa aatccgtatc taccgaatcg gaaaccgtta acacataagg cccgctataa
     6661 ttccacgcta cgcgtgcggc aaatcctttt ttctcgtaag tcaaagcaaa attgcccaca
     6721 tgattaggct gacccggcaa cgtattttta ttacccggcc tttgcggata gtaggcttgc
     6781 gaatgcgtat aggcatagtt caaataaatg ccaagcccgt caaaattggg aggtaaaaaa
     6841 gtaaatgttt gctgccaatt aaattcggca ccaaaaagtc gggcgacttt tccattttgt
     6901 ttttgattaa ctcgccactg attgacaccc cacggtgacc cctcattaag ggtagtgtct
     6961 atatattgac gccgatagat aaaatcattt aatactttat agtaaaagcc gccggctatg
     7021 ataccaacat cttttagata ataatcggcg gatatatcgt aattccaaga caccgtagga
     7081 cgtaaataag tattcccccg cgaaatcgta aaattttggt aatcaaaaat ttcgtaaggt
     7141 atcaaatcaa agtaatccgc cctgaatatg gcacgaccga tcgccgctcg aactgaaaaa
     7201 cggtcggtta tattgtactt cagattaaga gaagggaaaa actgatagta ttcataatgt
     7261 ccttttaacg atacggtatc aataggatat ccggcgaagt gcaatgtatc cggatagcct
     7321 aattgcgtgc ttcgaatagc ttttccgttt tgcactactt tgactccgtt ataatcaata
     7381 tatgtttttt ccatacgcac accagccaca acatccactc gattaacatt caatttagtc
     7441 attccataaa cggctatgat catttcgtca gcatcataat tggtaataaa actctcatcc
     7501 gcaaaatagt ttgcgctgtt aatcaagttg gccgtatcag cccaacgccg aatgagcgaa
     7561 ggatccgctg acgtgccgaa tcggtatcgc gaataaagaa aatttatgtc ttcatccgat
     7621 tccgcaaaaa aattcatgaa tggcggagca ttgggattcg ctatcgttgc acgattgtgt
     7681 tcatcattgc gttcttttgc cttggacctc acttttgcac cggactggac ataatcgccg
     7741 ttcgataata ttctcaaaga cttgcgaata ttcagctcag caatacgatc ccattccgta
     7801 atgtcgtatt cttgcacttc aaagttctta agtttgaaaa gggccgggtc ataaggatcg
     7861 ccggaaagaa acgcgaactt tggaaattga cggttcgtca tgtcgtattt aagattgaca
     7921 tccgtctgac gggtggtgaa atcaaagcga tccggaatcg cttctgttgc gcgcgataaa
     7981 gcggcactgt atgtccagtc catatctgcc cactgtgcgt gaccactcat ctgaaaactc
     8041 gtaaggcttc gggtcaacgc ccagtcttta gtttctcgct ctactacggc cgaatccatg
     8101 gttaccgatg aatccgacac aaatttaaaa cgtcgcgcac gaacaggagc ggtaccttcg
     8161 ctcagtttaa ttcgcaaacg acgacgtatc tcgtcatctc tgaaataatt atgtacggcc
     8221 ccgaagtgta tgtcgctcgt ttctgaaaaa cgataatctg ccgcggcatt gaagcttaac
     8281 cttttccgta ttgcattata gtctctgagt tcaaattcat tcatgaagta gtgattctgg
     8341 ccattgaaag cgcgttcacc ccaaacgcgt tccatgtcat cggaattgcg atgcgtgtaa
     8401 taacccatcg cattgacaaa cacgcctaat cttttttctt ccccgaaacg gttgccgtag
     8461 gttatatcaa aattggccag aggtttgcct tcaagcatat tacgacctgt cccggcggac
     8521 acttgccaca tttgttgatc aaaatccaac gcattttttg taaccagatt gatcgcaccg
     8581 gcgacggcat cggcatccat gtccggcgtg agcgttttgg aaacttcaat acgggatacc
     8641 atgctcgaag aaattacatc caaactaacc tggcgaatat cgccctccgg cgaaggcacc
     8701 ggttgaccgt taattttgac agacgaatat ttaggttcta atccgcgtaa aaaaacataa
     8761 cgcccttcgc cttgatcgcg cagtacggct acgcccggca tgcgttgcaa agcctcggaa
     8821 atattggggt ccggaaaaag tttgataatc tccgacgaag taacaacttg ctgattgtct
     8881 gcattttttt gaagatttaa tgctttcgcc tgaccttcgc gcataccata cacttcactc
     8941 ccggacaatt cgatactgcc ggccttaagc aaaatttcca tcttagtatg atcaccggca
     9001 gctacggtaa attttttttc gtaagttgta tagccgagat atgaaacttt gagtgtgtat
     9061 gaccccgatg caataccgct gatacgaaaa cgtccttccc tatcggatac agcacccaaa
     9121 gaagtgcctg ccaatttcaa atacgcgccg atcatcggtt cgctgtgcaa cgcacgactc
     9181 aaccggtagt accagcaacc ggttgtgtgg acctcgccgc ggtcggctgc ctgctgtagc
     9241 tcgggatcgg ctacgccggc gaggacatct aacagaagtg cgtcatcgac gaccagcgtc
     9301 acgggcggcg gttcagtcgc tgaccaggtc cggctcgtcc gcgacaaccg ctgcgacccc
     9361 agccgcatag gcgccgtcgg cgacggctgc gcgcaccgcc tcgcgttcgt cgtcggtcag
     9421 ccaagaaccg agctggcccg acggcggggg agtggcacga tgttcctcgg cgatcgcccg
     9481 gcgggcttcg gcgtcacgtt caccgacctc cccgacgaga c
//
LOCUS       DAOQNQ010000109         9505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2639_length_9505_cov_6.883492, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000109 DAOQNQ010000000
VERSION     DAOQNQ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9505
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2639_length_9505_cov_6.883492"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PK754_08150"
     CDS             <1..872
                     /locus_tag="PK754_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09514.1"
                     /translation="SLLRKLVDLNEKTGERVTLDQLILTYLHARLKAFLEDYEGPREK
                     HLESIKTRISHCHDMHRQFAITDLVEEHIFENPGLLLTLSEPDKYWPELINTFELNIM
                     LEIYIEREYLISGHHDMTKRQMMETLQQLINGIQEISIKSLSVVFLTAHLAAMQDHAK
                     SKTVSLDEIIQTIAQLRKDAEMAAKQITVESRVKHDILTTLTERLFRLETIQSAITLN
                     NTTDIVDRLDEVIVGLRTRRDNLIGGKMDAKVLQDDLNTYLHDSKVVGLIDEVERLYK
                     NELAQQAKIVASK"
     gene            complement(876..3185)
                     /locus_tag="PK754_08155"
     CDS             complement(876..3185)
                     /locus_tag="PK754_08155"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNB09515.1"
                     /translation="MMTFPARWTNHFRVAISQLLLFAGVVYFLFRWAEEDRALYLVLA
                     GITGTAFCVLALLHFTKTARIARRMSSVIIIAMTFCFVIVGYLFRGHIDHVIQEADLS
                     ANLDRLASYRANLNNRFLKHAEQGEFLRKRLTAEPFWRQPGILAEFLRHHTDDYFSVY
                     LLDDQGVSLLSEPVSTGYKESYPVRNLSNPAFVEPSFQPINDGNKVPAWHALKYFIPL
                     ETAEGQKRYVLLHIRTNAWLHEIKQSMPGVSVFLLHDNEIIATNEKHWNENIIREITN
                     EGYGRNSEEQIFFARYPLRSDNWSVVFLQTWNTAFPNVSYAESALVRVLIFSAVFGFL
                     TGVLLSWYLSRTILLPLSRLTNDVQIVVSGDQTHTLRESSSDPQDEIGALHRAIANIV
                     KSLKERTIQTESVQNAWSDVQSELEAQSDVVKKIQADTLRSSPLITRSLTINGRLVIA
                     QEHNGKFYDITEPQPGFAAFAIVDPAGKGAIGSFYAALMKGLLSAYWKRSDLLLQDPL
                     RWMNELETDFMKHRDTDTRTIALSVGIINTHDHRVRLINAGSEYPVLRKNDQTQSIAI
                     RGRALGMPQWLGSFETTTFVLALGETMFLHSHGISPDAMTEWPEKIHNTAMDSKQSDA
                     VILKIVRCESVKENFIFTPGSDVDFDAENKIQTMMARRGFESEKIKSMLSACNATITY
                     AARSTRLPELKNSIYVEAEACDDGIEVRITDQNNNSYWNMARIPEEFDQTESKGGRAW
                     NIGAIQKLVDEVIVEQSANKTTIVLRSIK"
     gene            complement(3604..3677)
                     /locus_tag="PK754_08160"
     tRNA            complement(3604..3677)
                     /locus_tag="PK754_08160"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3641..3643),aa:Ala,seq:cgc)
     assembly_gap    3775..3874
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4161..4733)
                     /locus_tag="PK754_08165"
     CDS             complement(4161..4733)
                     /locus_tag="PK754_08165"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB09516.1"
                     /translation="MIDFAEHAPLQSESGRLVLRDPYPEDAATMVMWRNESTVRAHQP
                     LRTVTVDTIEQDLLRSQSNVLPDYNRERFQWIIEKTEDRISIGWITLTIRSWEHEIGE
                     IGYSIATVFQGRGYGTEAIRLVLKKIFYEARLYRVEAKCSVENMASSRLLERLGFHRE
                     GIMREYFDIHGRRVDHYLYSLLRHEYFSKL"
     gene            4977..5162
                     /gene="rpmG"
                     /locus_tag="PK754_08170"
     CDS             4977..5162
                     /gene="rpmG"
                     /locus_tag="PK754_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011619264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNB09517.1"
                     /translation="MAKSKGNRIVITLECTSCKDSGLPGMSRYTTEKNKKNNTERLEV
                     KKYCNVERKHTLHKETR"
     gene            5191..5394
                     /locus_tag="PK754_08175"
     CDS             5191..5394
                     /locus_tag="PK754_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09518.1"
                     /translation="MAKKQTFVDKTAKVAAYAEKVKCEACKQESVASYAKFVESKVSE
                     KTGAWKFLERKVKLCGNCGTMIA"
     gene            5568..6395
                     /locus_tag="PK754_08180"
     CDS             5568..6395
                     /locus_tag="PK754_08180"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNB09519.1"
                     /translation="MNSGLARIVVAIVALPFVIAAIIYGSWYFFALMGTILLIGISEM
                     LNLLRHKNSKPQTFSAFFWGLIFLGLTFFGKIEFLLSALFLFAIYTMTVELFRNTGSD
                     MMNMAATWMTVFYPSLLIGSMILLRNTGASESHSGAQLVFLVFLSIWACDTFAYYGGR
                     MMGKNKFFERVSPKKTWEGAITGFFGALIGVWLVRYIYLTLDIPFIMSLPQTLVIGFF
                     GGTFGQIGDLAESLLKRDAGIKDSGALLPGHGGILDRFDSMMFVAPLTYIYVTYFVL"
     gene            6373..8058
                     /locus_tag="PK754_08185"
     CDS             6373..8058
                     /locus_tag="PK754_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB09520.1"
                     /translation="MSLILFYKSMRRWLIILVIIHLMGCVPHTPSDGGITTIALSSDP
                     ETLNPLFATGTNSSAVIYFLYPDLVEYDFNETTGRDTILPSLAKSWTISDDHSTLTYH
                     LHTHAHWHDGKKITAQDFAYTYRLYFDPQIGSARRTRIDIVQTRDDGSIDWENVIRTP
                     DDSTIVFYFKKNTPRHRMTEWAQQTPVPKHVLDTIPSSRLRTSWFNQKPVSGKHFTLE
                     RWERKQNLILKRNSEWTIPGKAHLDQIVFRIIPDMTTRLIELQSGRVDVVEGINPSDA
                     AELCKQSRFRIERQSGRRVEFVGWNHIDPSSYISSGTIKPHKLFSDQQVRKALSYAIH
                     RAEITQSWCGEYGQTASGPVSPVFLWAYNDQLPEMTYDPDSARIILQSAGWTDHDGDG
                     ILDKNGKPFQFTLTTNTGNPRREFIAQKIQSDLLRVGIKIEIQMLETAAFNAALRERK
                     LDAFITGYSVNMSLDLQPQFGSDLKKNTFNASGYQSAEADSLLNVIANAADRYSVADA
                     YRALQSVIYRDQPVTFLFWYDNLTVFNNRVRGTHADIYSPYHRFWEWYIEENP"
     gene            8063..9046
                     /locus_tag="PK754_08190"
     CDS             8063..9046
                     /locus_tag="PK754_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB09521.1"
                     /translation="MLRFILKRGRAAIPMIFGLLTVTFFLIRLAPGDPTSLFIDPSID
                     PQAAAQLKENLGLNDPLPVQYGKWLGVIPPFEGLLQGELGISFSKQMPVVEVISDALI
                     NTMILTVAALIVDILVGVGLGVVSALRRGTIFDRMTTVVGLFFYSMPHFWLALVLIMI
                     FSLYLNWFPASQMHSIGYEVWPFWTRVQDLLAHMVLPVFVLGIASAASTIRYVRSSML
                     EVLQQDYIRTARAKGLPESLVIWKHGLRNALLPLITIIGLSFPFLLAGAVVTEVIFAW
                     PGMGRVIIDAIFARDYPLIIANTMMAGILVILGNLLADILYAVADPRARAE"
     gene            complement(9054..>9505)
                     /locus_tag="PK754_08195"
     CDS             complement(9054..>9505)
                     /locus_tag="PK754_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09522.1"
                     /translation="GDYEDALSHYESLIATEGSTARVGAGWSAMRLNDLNTANVYFSE
                     AALDSIPDGYAGWSVTLWGSGSLLQSLDKTAFVLRKKPEFKLSLDRSVTHKDLIWVQA
                     SCYYQLGNYPAAVNRIQALDDTFAPNMEAANLAEVIALKLQALGVVH"
BASE COUNT         2612 a         2200 c         1973 g         2620 t
ORIGIN      
        1 gttcgttgtt gcgcaaatta gtggatctca acgaaaaaac aggcgaacgg gtgacattgg
       61 atcaattaat tctcacctat ttacacgcgc gcctcaaagc ttttctcgaa gattacgaag
      121 gcccgcggga aaaacatctt gagtctatca aaactcgcat ttcccattgt catgatatgc
      181 accgtcagtt tgccatcacg gacctagtag aagaacatat cttcgaaaat ccgggattgc
      241 tgttgacgct ttccgaaccg gataaatact ggccggaact catcaacaca tttgaactca
      301 atatcatgct ggagatttat atcgagcgcg agtatttgat ctccggtcat cacgatatga
      361 ccaagcgtca gatgatggaa actttgcaac agctcatcaa cggtattcag gaaatttcca
      421 tcaaaagtct cagcgtggta tttctcacag cgcatctggc cgccatgcaa gatcatgcca
      481 aaagtaaaac tgtttccctt gacgaaatca tccagactat cgctcaactc cgcaaagatg
      541 cggaaatggc cgctaaacag atcaccgtgg agtcgcgcgt caaacatgat attcttacga
      601 cattgacaga acggttattc cgtttggaaa cgatacaaag cgctatcaca cttaataaca
      661 ccacagatat cgtagatcgc ttagatgaag tgattgtcgg tttacgtacg cgcagagata
      721 atctgattgg cgggaaaatg gatgccaagg ttttgcaaga tgacctgaat acttatctgc
      781 atgactcgaa agttgtcgga ttgattgacg aagtagaaag actctataaa aacgaacttg
      841 cccaacaggc caaaatcgtt gcatccaaat agtcgttatt ttattgatcg taaaactatc
      901 gttgttttat tagccgattg ctctacgatt acttcatcaa cgagtttttg tatggcaccg
      961 atattccacg cacgcccccc tttgctttcg gtttgatcga attcttccgg gatcctcgcc
     1021 atattccaat acgaattgtt attctgatcc gtgattcgca cttcgatgcc gtcatcacac
     1081 gcttcagctt cgacataaat cgagtttttc aactcaggca gcctggtact ccgggcagca
     1141 taggttatcg ttgcgttgca agcggataac atggacttga ttttttccga ttcaaaacca
     1201 cgacgcgcca tcatcgtttg gattttattt tccgcgtcaa aatctacatc cgaccccggt
     1261 gtaaatatga aattctcttt aacgctttca cagcgaacta tttttaatat gacggcgtca
     1321 ctctgtttcg aatccatcgc cgtgttatga attttctccg gccactcggt catcgcgtcg
     1381 ggcgaaattc catggctgtg taaaaacata gtttcaccca aagccaatac aaatgtcgta
     1441 gtttcaaaag aaccaagcca ttggggcatg cccagagctc tgccgcgtat ggcgatagat
     1501 tgcgtttgat cgttttttcg caaaacgggg tattccgacc ccgcattgat caaccgtact
     1561 cgatggtcgt gggtattaat gattccgaca ctaagtgcta tcgttcgcgt atcggtatcc
     1621 cgatgtttca taaaatccgt ttcaagttcg ttcatccatc gcaaagggtc ttgcagaagc
     1681 aaatcactgc gtttccaata cgcactcagc aatcccttca tcagtgcagc ataaaacgaa
     1741 ccgatggcac ctttaccggc aggatcaacg atagcaaaag cagcaaaccc cggttgcggt
     1801 tcggttatat cgtaaaattt tccgttgtgc tcttgggcta ttactaaacg accattgatc
     1861 gtcagactac gcgttattaa aggcgatgac ctaagagtat cggcttggat tttttttacg
     1921 acatcggatt gagcctccaa ttccgattgc acatcagacc atgcgttttg aacggattcg
     1981 gtttgtatcg ttctttcctt tagggatttt actatgtttg ctatagcgcg atgaagagcg
     2041 ccgatctcat cttgtggatc agaggaagat tcgcgaagtg tatgcgtttg atcaccggac
     2101 acaacaatct gaacatcgtt tgtcaaccgg ctcaagggta aaagaatagt acgactgaga
     2161 taccaactca ataaaactcc ggtcaaaaat ccaaatacgg ccgaaaaaat cagcacacgt
     2221 accaatgccg attcggcgta tgacacatta ggaaaggccg tattccatgt ctgtaaaaaa
     2281 accaccgacc agttatcact gcgcagcggg tatcgcgcaa aaaaaatctg ttcttcggaa
     2341 ttacgcccat atccttcgtt tgttatttcg cgtattatgt tttcattcca gtgtttttcg
     2401 ttggtcgcga tgatctcgtt atcatgcagc aaaaaaacag aaacgcccgg catggattgt
     2461 tttatttcat ggagccaagc attcgttctg atatggagta aaacataacg tttttgacct
     2521 tctgcggttt caagagggat aaaatatttg agcgcatgcc acgctgggac tttattgcca
     2581 tcgtttatag gttggaatga cggttcgacg aatgccggat tggacaaatt acgaacgggg
     2641 tagctttctt tgtatccggt tgataccggt tcagacaaaa gacttacacc ctgatcgtct
     2701 aagagataaa cagaaaaata gtcgtcggta tgatgcctca ggaattcggc taatataccc
     2761 ggttgcctcc aaaacggttc cgcagtaagc cgttttctca aaaactcgcc ttgctcggca
     2821 tgcttcaaaa agcgattgtt taagttggcc cgataggacg caagacgatc aaggttggcc
     2881 gacaaatccg cttcctgtat cacgtgatcg atatggccgc gaaaaagata acctacgatt
     2941 acaaaacaaa atgtcatagc gataatgatt acggaactca tgcgccgtgc aatgcgtgct
     3001 gttttagtaa aatgcaacag cgccagcaca caaaaggccg taccggttat acccgccaaa
     3061 actaaataca atgcacgatc ttcttccgcc caacgaaaaa gaaaatacac gacaccggcg
     3121 aaaagcagta actgagagat tgcaacacgg aaatggtttg tccaccttgc aggaaaggtc
     3181 atcattgcgg agtgttcctc ttaataaaac caacgtacac aattcgttcc tgtaattcaa
     3241 cttgggatat tgatgctgac catagcggta cttgaacaaa ttaaaacacg tgctactgat
     3301 gtgctccgcg aaggttaata tgcagtatgt tttttcgttg gcaacagata aaacaaaaaa
     3361 ccaataaact tctttgttca ttggttttta ttattttcga cgtggagctg agggggatcg
     3421 aacccctgac cttcccgcct tcgagcggga cgctctccta agaataacta aataagatga
     3481 gcactttttc ttccacttga tttcatagga tagagttctt agtaaatcat agtcaataaa
     3541 actatggaac gagtggatag aatttgcctc gtttgtgttc cgcaatacaa aacgtaaacc
     3601 atgtggagct gagggggatc gaacccctga cctcatcatt gcgaacgatg cgctctccca
     3661 agctgagcta cagccccaat atcaaaacaa tttcaaagat acgtttaaaa atttaccaaa
     3721 tcgaaccact gaccttcccg ccttcaagcg ggacgctctc ctcacgccca cggcnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaacaat ttcaaagata cgtttaaaaa
     3901 tttaccagat cgaaccactg accttcccgc cttcaagcgg gacgctctcc tcacgcccac
     3961 ggcgtgaagc tacagcccca atatcaaaac aatttcaaag atacgtttaa aaatttacca
     4021 gatcgaacca ctgaccttcc cgtcttcgag cgggacgcac tctaaattta atctacggcc
     4081 aataaaatat tttaaaacga tatttttata tttaacgatg gcgcaatcta aatgaccgca
     4141 tgtaaaaata caatgaattt ttacagcttg gaaaaatact cgtgccgtaa cagactgtat
     4201 aagtaatgat ccacccgtcg cccatggata tcaaaatatt cgcgcataat accttcacga
     4261 tgaaaaccca atcgttccag caaacgtgaa gacgccatat tttcgacaga acatttggcc
     4321 tcaacccggt acaaacgggc ttcgtaaaat atttttttta acacaagccg tatcgcctcc
     4381 gtcccgtaac cgcgtccctg aaaaacggtc gcaatactgt aaccgatttc tccgatttcg
     4441 tgttcccagg aacgtatcgt cagcgtaatc cagccgatgg atatacgatc ttctgttttt
     4501 tctatgatcc actgaaaccg ctcacgatta taatcgggta aaacattgga ctgcgaacgt
     4561 aacaaatcct gctctatcgt gtccacggtg accgtacgca atggctggtg cgctcttacc
     4621 gtggattcat tgcgccacat caccatagtc gccgcatctt ccggataagg atcgcgcaat
     4681 actaaacgcc ctgactcact ttgtaaaggg gcatgttctg caaaatcaat cataattcag
     4741 ttactcaaac cgattttgta aaatgttaat ccattgaaat ctaaagcgca tcaaaaaata
     4801 caattctata taactaatcg caagattaag cttatcaaaa tcacctaaac ctataaaaaa
     4861 cagcgcacga aaaatcaata ttattacttg acaaacgaaa gcttttaata tatatttctg
     4921 cccattttgg atagaatata attttattat aaacatagtc ttttggagag ttcgtcatgg
     4981 caaagtctaa aggcaatcgc atcgtgatta cgctggaatg cacatcctgc aaagacagcg
     5041 gtttgcccgg tatgtcccgt tataccacgg aaaagaacaa aaaaaataat acggaacgcc
     5101 ttgaagtaaa aaaatactgc aatgtagaac gtaaacatac cctgcacaag gaaacccgtt
     5161 aataagcatt ttttgtaaag gagctttgcc atggcaaaga aacaaacatt tgttgataaa
     5221 accgctaaag tcgcagcgta tgctgaaaaa gtaaaatgcg aagcctgcaa acaggaatct
     5281 gttgcatcgt acgccaaatt cgtagaatcc aaagtatcgg aaaaaaccgg tgcatggaaa
     5341 tttttggaac gcaaagtaaa actctgcggt aactgcggca ccatgatcgc ctgatctatt
     5401 caaatttagt ttttaaaagc ccgtatcgat tgacgatatg ggcttttttt ttaatgcatt
     5461 gccgggtatt ttacttgcca ttccttatac ggcgatctat attttgatgc acatcacata
     5521 attttatttt caatcaacgg tcttgtacat caaacaacga ggtttatgtg aattcaggac
     5581 tcgcccgcat cgtggtcgcg attgtagctt tgccttttgt catcgccgcc atcatttatg
     5641 gttcttggta tttttttgca ctgatgggca ctatattgct catcggcata tccgaaatgc
     5701 taaacctgct tcgccacaaa aacagtaaac cacaaacttt ctcagctttt ttttggggat
     5761 tgatttttct agggctcacg tttttcggca aaattgagtt tttattatcc gccctttttt
     5821 tgtttgcaat atataccatg accgtggaat tgtttcgcaa caccggttcg gatatgatga
     5881 atatggcggc cacatggatg accgtttttt atccgtcatt attgatcggc tcgatgattt
     5941 tactccgtaa taccggcgct tcggagagtc atagcggcgc tcaattagtg tttttggttt
     6001 tcttgagcat ttgggcttgc gatacatttg cgtattatgg cggccgaatg atgggtaaaa
     6061 ataaattttt cgaaagggtc agccctaaaa aaacatggga aggcgccatt accggctttt
     6121 ttggtgcatt gatcggcgta tggctcgttc gctacatcta tctgacgctt gacatccctt
     6181 tcattatgag tttgccgcaa acattggtaa tcggtttttt tggcggtaca ttcggacaga
     6241 tcggtgatct tgccgaatct ttgctcaaac gggatgccgg catcaaagac agtggcgctt
     6301 tgctccccgg gcacggaggc attctggatc ggtttgacag tatgatgttt gttgcaccgc
     6361 tcacctatat ttatgtcact tattttgttt tataaatcca tgcgccggtg gcttattatt
     6421 ttggtgatta tccatctgat gggatgtgtg ccgcacacgc cctcggacgg cgggatcaca
     6481 actatcgctt tgtcatccga tcctgaaacg ctaaatcctc tttttgccac gggtaccaat
     6541 tcttccgcag tgatttattt tttatatccc gacttggtag aatacgactt taatgaaacc
     6601 accggacgcg acaccattct cccgtcatta gcaaaatcct ggactatttc ggatgaccat
     6661 tctacgctga cttaccattt gcatacgcat gcacattggc atgacggaaa aaaaatcacg
     6721 gcacaggatt ttgcgtacac ttatcgttta tacttcgatc cgcagattgg gagtgcaagg
     6781 cgaacacgaa tcgatatcgt acaaacccgt gacgatggct cgattgattg ggaaaatgta
     6841 attcgcacgc cggatgactc cacgatcgtt ttttatttca aaaagaacac gccacgccac
     6901 cgtatgaccg aatgggcaca acagaccccc gttcccaaac acgtgctgga tacaatcccg
     6961 tcttcgcgtc ttcgtacatc ctggtttaat cagaaaccgg tgagcggaaa acattttacc
     7021 ttggaacgat gggaacgaaa acaaaacctg attctcaaac gcaattcgga atggacgata
     7081 cccggcaaag cccatcttga tcagatcgta tttcgtatta ttcccgatat gaccacgcgc
     7141 cttatcgaat tacaatccgg acgtgtggat gttgtcgaag gcattaaccc gtccgatgcc
     7201 gccgagctgt gcaagcaatc ccgttttaga atcgaacgtc aatccgggcg ccgtgtcgag
     7261 tttgtagggt ggaatcatat tgatccatcg tcctatatta gctccggaac gataaaaccg
     7321 cacaaacttt tttccgatca acaggtacgc aaagcattga gttacgcgat tcatcgcgca
     7381 gagatcacac aaagttggtg cggagaatac ggccaaacag catcaggtcc ggtatcgccg
     7441 gttttcctat gggcatataa cgatcaactt ccggagatga cctacgaccc ggattcggca
     7501 cgcattatcc ttcaaagtgc aggatggacc gatcatgacg gtgacggtat tcttgataaa
     7561 aatggtaaac cttttcaatt tacactcaca accaataccg gcaatccacg ccgcgagttt
     7621 atcgcacaaa aaatccaatc ggatctgttg cgcgtcggca tcaaaataga aatccagatg
     7681 ctcgaaacgg cagccttcaa tgccgcgtta cgtgagcgaa aattagatgc attcatcacc
     7741 ggctactcgg tcaacatgag ccttgacctg caaccgcaat tcggttccga tctgaaaaaa
     7801 aatacattta acgcatcagg ttatcagagt gctgaagcgg acagtttact aaatgttatc
     7861 gccaacgcag cagatcgtta ctctgtagcc gacgcttatc gcgcattgca atccgttatt
     7921 taccgcgatc aacctgtaac ttttttattt tggtatgata atttgactgt gtttaataat
     7981 cgtgtccgcg gcacacacgc agacatttat tcgccatacc accgtttttg ggaatggtat
     8041 atcgaagaaa acccatagcc gtatgttgag atttatatta aaacgcgggc gcgcggcgat
     8101 cccgatgatt ttcggattgc tcacggtcac ttttttttta atacggttag cgcccggcga
     8161 tccgacatca ctttttattg acccatccat agatccgcaa gcggcggctc aacttaaaga
     8221 aaacctcggc ctcaatgatc ctttacccgt tcaatacgga aaatggttgg gtgtcatacc
     8281 gccgtttgaa ggcttactac aaggcgaact gggtatttcg tttagcaaac aaatgcctgt
     8341 tgtagaagtg atttcggacg cgttaatcaa tacgatgata ctaaccgttg ctgcattgat
     8401 cgtggatatt cttgtcggcg tcggactcgg tgtggtttcg gcattgcgca ggggtacgat
     8461 ttttgatcga atgacaaccg tcgtaggttt gtttttttat tcgatgccac atttttggtt
     8521 agctctggtt ttgattatga ttttttcact ttatttgaac tggttcccgg cttcacaaat
     8581 gcacagcatc ggttacgaag tgtggccctt ttggacacgc gtgcaggatt tgctggcgca
     8641 catggtgtta ccggtttttg tgttggggat tgcttctgcc gcttcgacga tacgttatgt
     8701 gcgcagtagt atgttggaag ttttacagca agattatatc cgcacggcgc gtgccaaagg
     8761 attgcctgag tctttggtaa tatggaaaca cggtttacgc aacgcgttac tacctttgat
     8821 cacgatcatc ggattatcgt ttccattttt attggcgggc gctgtcgtta cggaagttat
     8881 ttttgcgtgg ccggggatgg gacgggttat tattgatgcg atttttgcac gggattatcc
     8941 gctgattatt gccaatacca tgatggccgg tatattagtt atcctaggca atcttttagc
     9001 tgatatattg tatgccgtag ccgatccgcg tgcacgtgcg gaataatatt gttctagtgt
     9061 acgacaccca atgcctgaag ttttaatgcg atgacttccg ctaaattggc cgcctccata
     9121 ttcggagcaa acgtatcatc caacgcctga attctattga ccgccgcagg ataatttcct
     9181 aactgataat aacagctggc ctgaacccat atcaggtctt tatgtgtcac gctacgatca
     9241 agcgagagct tgaactccgg tttttttcgc aacacaaagg ctgttttgtc caatgactgc
     9301 aaaagacttc cggaacccca cagtgtaaca ctccaaccgg cgtatccgtc cggtatcgaa
     9361 tccaaggccg cttccgaaaa ataaacattc gccgtattca gatcgttgag ccgcatcgca
     9421 gaccaacctg caccaacacg cgccgtcgaa ccttcggtcg caatcaaact ttcataatga
     9481 cttaacgcat cttcataatc accgt
//
LOCUS       DAOQNQ010000110         9418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2680_length_9418_cov_5.447827, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000110 DAOQNQ010000000
VERSION     DAOQNQ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9418
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2680_length_9418_cov_5.447827"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="PK754_08200"
     CDS             complement(<1..1452)
                     /locus_tag="PK754_08200"
                     /inference="COORDINATES: protein motif:HMM:NF023650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HNB09523.1"
                     /translation="MRKKIGVTLTMADTTEVEYIIDQNQTQVNLSSAPSLQVRTYIPH
                     KIMRSLYYFVLASGLILFIVSVNNLYLNNRIPWNTMVHDVSVGGMTVSQAREALTVYV
                     DEFKNRDVRLLTEEGEFLTKNTEFQPTFDIEKAIVTAFERGHNPDPLKNVQTRTASMF
                     FSVRIPLESWYDENRFVRTIINKIPKLKNNQPIDATVAWADRQFKAVAGRGGLGFDEE
                     KAKKSFSENIRSFASKDIPVDILSKEPDVTIAATTKAIRRAYAAYGKNVRMYYTYDGY
                     NYDSWAFAISDRREFIEFRKVWEGNEFVLYPVLNAEMLREELNRRVAPYMYRAKEDVT
                     IRDDNGKIVVEGVARDGYYLDIPRSIININESVMKGILDTVGNIRADLVVAHLTGGIS
                     NPDNPFGITDVLATGVTDYFGSPENRKFNINHAAKNFQNIVVKPGEDFSFLKYLGEVD
                     SLSGYLKELVIVNGDSSEPNWGGGVCQVSSTLFR"
     gene            1595..3154
                     /locus_tag="PK754_08205"
     CDS             1595..3154
                     /locus_tag="PK754_08205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB09524.1"
                     /translation="MRVSLEKKILVTAAASILFITGIAIFGFHYFSSRLSEQLIDNKI
                     GQALASGRQIAENLIEDERRIFSENARAFSQRPEFTDFIIRLQGQKLTSDQFQAFIGL
                     PLFTQLRVDGVFLYDEKGRCLFRHLSILDKRLSSVFPDSLKTDERFARSTSAYDSINR
                     SLVYKTTLALPSQQQGYLITARMVEKEFAKKYERIVAANNQYALLLRDADNMRFTHLW
                     IFITAVLAPAFIFILLLWFFIGQMFKPFQALSRQASNIAKGQMDYSLQIDTRDEFGAL
                     AAALNQMVQNLRDEQQRILQLEKTATWREMAQRMAHEIKNPLTPIQLTIQQIKDSYAG
                     NDEKYRKLLTECTTIIEEEIETLRNLTKEFSDFARMPVVVLKPSSLNKLIEDIILMYA
                     AIPFEKKLDDRLPFIPLDTEAFKRVLINLIDNALAAVSNKQDRLIRIATIDVNTHVQL
                     IIADNGYGIPKSNLERIFEPHFSTKTTSMGLGLAIVKKIIEEHQGTISVDSIENLGTT
                     FTITLPKENPS"
     gene            3167..3577
                     /locus_tag="PK754_08210"
     CDS             3167..3577
                     /locus_tag="PK754_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNB09525.1"
                     /translation="MAYWIVKSEPSVYSFEQFQKDKTTVWDGVKNPAALINLRSMKKG
                     DTVLFYHSNDGKCLVGTAVVNKEAYPDPKSKNPKLVVVELSVGKALAKPVTLAEIKSN
                     TKLAQLELVRISRLSVSKVPDTLWKELMKMAGEK"
     gene            complement(3578..4471)
                     /locus_tag="PK754_08215"
     CDS             complement(3578..4471)
                     /locus_tag="PK754_08215"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNB09526.1"
                     /translation="MKSNAPVQQTDFKNLKLMRRGKVRDVYDLGDKLLIVVTDRISAF
                     DVIMKDTIPQKGVVLNTMASFWFDQTRHIIRNHVITTDVEKYPEPCKAYRDELQGRSM
                     LVEKAKSLPVECIVRGYISGSGWNEYQKTGAVCGVVLPQGLKESDKLPEPIFTPTTKA
                     EQGHDQNITFDEMCKTISKDVAEQVRATSIKIYQFAQTLAASKGIIIADTKMEFGIKD
                     GELILIDELLSPDSSRFWPADQYKPGDKQPSYDKQFLRDYLLAIKWDNNTAAPILPED
                     IIKKTGEKYREALRLITGINL"
     gene            complement(4468..4986)
                     /locus_tag="PK754_08220"
     CDS             complement(4468..4986)
                     /locus_tag="PK754_08220"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB09527.1"
                     /translation="MSRQIIVTTPRLTLQALDIESLKLLSENRNKFAQHLGVNVPEQW
                     PALDLIEALPFFIGLIEQDPSEYMWLVWITVETKSKNIVGGIGFKGAPDIQGTVEVGY
                     DTAEMYRGFGLATEALQGITKWAFEQSEVRRVIAETDQSNFQSHRVLEKAGYLKSGEN
                     ETMFFWERLRSL"
     gene            complement(4971..8015)
                     /gene="secA"
                     /locus_tag="PK754_08225"
     CDS             complement(4971..8015)
                     /gene="secA"
                     /locus_tag="PK754_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNB09528.1"
                     /translation="MIQSLLSKIFGNKHERDVKKLYPIVDAINQNFQQFDTLSDDELR
                     AKTNEFRDRIRENTKEEIAAVQAIKETIRTNTDPNRSKTLVEELKVAEKTLREAEQYI
                     LDEILPEAFACVKQACKRLVGQKYMVCGREVKWDMVPYDVQMIGGIVLHEGKISEMST
                     GEGKTLVAVAPVYLNALAGHGVHLVTVNDYLALRDKEWMGKVYEFLGLTIGVILNDMN
                     PTQRRVAYGCDITYGTNNEFGFDYLRDNMATSPDDVVQIRGHHYAIVDEVDSVLIDEA
                     RTPLIISGAVDTPTNQKYEESNPGVRRLVDLQTRLVSQLVADADKLITEGKNKEAGIK
                     LLMAQRGAPKNKRLMKAMTEEGVAQLIQSVESEYLRDKKMHEIDEELFFAIDEKNHGI
                     DLTEKGRNALSPSNPEMFVIPDLSEEIIKIDGNKELTDHQKAIKKEELGTLYAERSDR
                     IHSIEKLLTAYSLYEKDVEYVVQDGKVQIVDEFTGRILHGRRYSDGLHQAIEAKENVK
                     VEKDTQTLATITLQNYFRLYSKLAGMTGTASTESTEFYEIYKLDVTAIPTNKPIQRED
                     FEDMIYRTKREKYNAVIELIRENHKAGKPTLVGTTSVEVSETLSRMLSRTGIKHEVLN
                     AKQNQREAEIISNAGQKSAVTIATNMAGRGTDIKLGSGVVEAGGLQIIGTERHESRRI
                     DLQLRGRSGRQGDPGSSVFFISLEDDLMRLFGSDRIARVMDKLGAEEGEVISHRWITK
                     AVERAQKRVEFRNFSIRKRLIEYDDVMNQQREIIYSRRRRALMGENIKVEFLDVLDEF
                     VEMLNDRFVKNQPHPEDWDLVGLTETLLRSCGIAVAQDEFYKLNAEQFSVHIRGMLME
                     RYQQREEVWSSENMRRIERIAVLKTIDERWKEHLREMEEFKEGIHLRGYGQKDPLIEY
                     KTEGFEMFMQLLDRITSEIVEFVFRAEPAAEMEQQMIRRARAMKAVHASSEGMGFSGS
                     GEEQAERPVQSTTTPIRAEQKVGRNDPCPCGSGKKYKQCHGK"
     gene            complement(8046..8747)
                     /locus_tag="PK754_08230"
     CDS             complement(8046..8747)
                     /locus_tag="PK754_08230"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNB09529.1"
                     /translation="MRIAIISDIHGNIEALRQALDIIGELGTDEIVCLGDIVGYGPDP
                     EPCVDLIRKHCKTVIMGNHDNAVIDMSATENFNPVAKQAVWWTHQQLSPESLSFLQGL
                     PYRNQRQDLLFVHASPMNPAKWTYLFTINDALPEFDAFQERICFVGHTHSPRVFCNSP
                     HATAITREFQFIINVGSVGQPRDGDARLSFGLFDTEKWDYKNIRSEYDVQTTARKTRE
                     RGLPVILAERLLRGR"
     gene            complement(8749..>9418)
                     /locus_tag="PK754_08235"
     CDS             complement(8749..>9418)
                     /locus_tag="PK754_08235"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNB09530.1"
                     /translation="NDLLSTDVTIGYNTAVTIAVDALDKLHTTAKSHHRAMVVEVMGR
                     YTGWIALEAGISGAVDVILIPEIPFEISKVCAHLQKRIEQGKNYSIIMVAEGAMPVGG
                     KTMILETAEQNVGGAERLGGIGNWVSAQIEKESGVESRCVVLGHIQRGGPPSHIDRLF
                     ASRMGAYAVELAQQKMFGYMASARNKEIIHVSIENAVEKIRYINPDSETVKSARQIGI
                     SFGN"
BASE COUNT         2488 a         2247 c         1891 g         2792 t
ORIGIN      
        1 tctaaaaagt gtacttgaaa cctgacatac tcccccgccc caattaggct cgctgctgtc
       61 tccgtttact atcacaagct ctttgaggta tccggaaaga ctgtccactt caccgagata
      121 ttttaaaaat gaaaaatctt cgcccggttt aaccacaata ttttgaaaat tttttgctgc
      181 gtggttaata ttaaatttac gattttccgg tgaaccaaaa taatccgtta cgccggttgc
      241 taatacgtcc gtaataccaa agggattgtc aggatttgaa atcccgccgg taagatgagc
      301 tactactaaa tctgcgcgaa tattgccgac cgtatccaat atccccttca tgacggattc
      361 gttgatattg atgatcgagc gcggtatgtc aagataatat ccatctcggg caacaccttc
      421 aacaactatc ttaccattgt catcacgaat tgtaacatct tctttagcac gatacatata
      481 cggcgcaacg cgacgattga gctcttcacg caacatctct gcattaagca ccggatataa
      541 aacaaattca ttgccctccc acactttgcg gaattcgata aattctctcc gatcagatat
      601 tgcaaaagcc cacgaatcgt agttatagcc atcgtatgta taatacatac gaacgttttt
      661 cccatacgcc gcgtaggcac gtcgaatggc tttggttgta gcggctatcg tgacatccgg
      721 ttctttacta agtatatcta caggtatatc tttggacgcg aaggatcgga tattttcaga
      781 aaatgacttt tttgcttttt cttcgtcaaa tccaagtccg ccccgccctg caacagcttt
      841 gaattgacga tcggcccaag ctacagtagc gtcaatcggt tgattattct taagctttgg
      901 aattttgttg atgatagttc ggacaaaacg attttcgtcg taccaacttt ctaacggaat
      961 tctaactgaa aaaaacatag atgccgtgcg tgtttggacg tttttcaacg gatccgggtt
     1021 gtggcctcgt tcgaaagctg ttactatggc tttttctata tcaaaagtcg gctgaaactc
     1081 ggtattttta gttaggaact cgccttcctc cgtcaataat cgaacatctc gatttttaaa
     1141 ctcatctaca taaacagtca atgcctcgcg cgcctgtgaa acggtcatac ctccgactga
     1201 aacgtcatgg accatagtgt tccatggaat cctgttattg agatacaaat tatttaccga
     1261 aacgataaat aaaatcaatc cgctagctaa aacaaaataa tacagcgacc tcataatttt
     1321 atgaggaatg tacgtacgta cctgtaaact cggcgccgac gaaagattga cctgagtttg
     1381 attttgatca attatgtatt ctacttcagt tgtgtcggcc atcgtcagag ttacccctat
     1441 cttttttcgc aaaaaacgta ataaataggt taaatacgcg cttgtataac gtaaaaaaac
     1501 tttttaaact ggtgcaatat aatttattcc gccatacatc tgcaagaact atcgtgtatg
     1561 tgtacaataa cgttatcttt ggtttaatgc tcatatgcgg gtttctttag aaaaaaagat
     1621 tttagtcacc gccgccgctt cgatactgtt cattaccggt atcgcaattt tcgggtttca
     1681 ttatttttca tcacgccttt ccgaacaact gatagacaat aaaatcggac aagcgctggc
     1741 tagcggccgt caaatagccg aaaatctgat cgaagacgag cgtcgcatct tttcggaaaa
     1801 tgcgcgtgcc ttttcacaaa gacctgaatt cactgatttc attatccgtt tgcaaggtca
     1861 aaaactgacc tccgatcagt ttcaggcgtt tatcggtttg cctttattta ctcaactccg
     1921 tgtagatggc gtttttttgt atgacgagaa aggtcgttgt ttattccgac atttaagcat
     1981 actagacaaa cgtctttcgt ccgtatttcc cgatagtctc aaaacagatg aacggtttgc
     2041 aagatctacc tctgcttacg acagtatcaa tcgatccctc gtgtacaaaa cgacactggc
     2101 gttgccttca caacagcagg gttaccttat cacggcgcgg atggtggaaa aagagttcgc
     2161 caaaaaatac gaacgtattg tcgcagcgaa caatcaatat gctttgctgc tccgcgacgc
     2221 agataacatg cgctttactc atctatggat attcatcacc gcggtacttg cacctgcatt
     2281 tatatttatt ttacttttat ggttttttat cggacagatg ttcaaaccgt ttcaggcgct
     2341 tagtcggcag gcttccaata ttgctaaggg acaaatggac tatagtttac aaattgacac
     2401 aagggatgaa tttggtgctt tggctgccgc tcttaatcag atggttcaaa acctccgtga
     2461 tgaacagcag cgcattttac aacttgaaaa aacagcgaca tggcgtgaaa tggcgcaacg
     2521 tatggcgcac gagataaaaa acccgcttac tccgatacaa cttacgattc agcaaattaa
     2581 agattcatac gccggaaatg atgaaaaata tcggaaactt ctcacggaat gcacaactat
     2641 catcgaagaa gaaattgaaa cattgcgcaa tttgaccaaa gaattctctg attttgcgcg
     2701 catgccggtc gttgttctta agccgtcgtc tcttaataaa cttattgaag atatcatcct
     2761 gatgtatgca gccatcccct tcgaaaaaaa attagatgat cgccttccat ttattcccct
     2821 tgataccgag gcattcaaac gcgtattaat taacctgatt gataatgcgc tggccgcagt
     2881 ttcaaacaaa caggatcgcc tgattcgtat agcaacgata gatgtgaaca cacacgtaca
     2941 acttattatt gccgataacg gatacgggat tcctaaatcc aatctcgaac gtatttttga
     3001 acctcatttt tcaacgaaaa caactagtat gggacttggt ctggcgatcg taaaaaaaat
     3061 aatcgaagag catcaaggca ctatctctgt cgatagcatc gagaaccttg gaacaacttt
     3121 taccatcact ttgccaaaag aaaatccatc ttaaaggaga gtatatatgg cgtattggat
     3181 cgtcaaatca gaaccgtccg tttattcatt tgaacaattc caaaaagaca aaacaacggt
     3241 ttgggacggt gtcaaaaacc cggccgctct tattaatttg cgatccatga aaaagggcga
     3301 taccgtactt ttttatcaca gcaacgatgg taaatgcctt gtgggaacag ccgttgtaaa
     3361 taaagaagcg tatcctgacc ctaaatcgaa aaaccctaag cttgttgtgg tggaactgtc
     3421 ggtgggtaaa gctttagcta agccggtaac tcttgcagaa atcaaatcta acacaaaact
     3481 cgctcagtta gagttggttc gtatcagccg cttgtctgtc agcaaagtac cggatactct
     3541 ttggaaagaa ttgatgaaaa tggccggtga aaaatgatta taaatttata ccggtgatca
     3601 gccgcagcgc ttctcgatat ttctcgcctg ttttcttaat aatatcttcc ggcaaaatgg
     3661 gtgcggctgt attattatcc cacttaatag ctaacagata atccctaaga aattgtttgt
     3721 cataactcgg ctgtttgtca cctggtttat attgatcagc gggccaaaat cgcgatgaat
     3781 ccggagataa aagctcatct atcaatatca attcaccatc tttgatgcca aactccatct
     3841 tcgtatccgc aatgataata cctttggaag ccgccagagt ttgagcaaat tgataaatct
     3901 taatgctcgt agcgcggact tgttcagcaa catctttgct tattgtttta cacatttcat
     3961 caaaggtaat attctgatcg tgaccttgtt ccgctttcgt cgtcggcgta aaaataggtt
     4021 ccggcagttt atccgattct tttaaacctt gaggtaaaac gacgccgcag acggctccgg
     4081 ttttttgata ttcgttccaa cctgagcctg aaatatagcc acggacaatg cactctaccg
     4141 gcagcgattt cgctttttcg acaagcatac ttcgtccttg aagttcatca cggtaggcct
     4201 tgcaaggctc cggatatttt tcaacatccg tagtgatcac atggtttcga ataatatgac
     4261 gtgtctgatc aaaccaaaat gaggccatcg tattgagtac tacgcctttt tgcggtatcg
     4321 tatctttcat gatcacgtca aacgctgata tgcgatctgt cactacgatg agcagtttgt
     4381 caccgagatc atatacgtca cgaacttttc cgcgacgcat caattttaag tttttaaaat
     4441 cagtctgctg taccggtgca ttagacttca taatgatcta agcctttccc aaaaaaacat
     4501 tgtttcattt tcaccggact ttaaatatcc tgctttttcc aatacacgat gggattggaa
     4561 gtttgattga tcagtttccg ctatcacccg acgaacttca ctttgctcaa aagcccattt
     4621 tgttatgcct tgtaatgcct ctgtagcaag gccaaaacca cggtacattt ccgccgtatc
     4681 gtagcctact tctacggttc cctgtatatc tggcgctcct ttaaacccga tcccgcctac
     4741 gatatttttg gattttgttt cgacagtaat ccataccagc cacatgtact cgcttggatc
     4801 ctgttcgatc aaacctataa aaaatggcaa cgcttcaatc aaatcgagtg caggccattg
     4861 ctccggaaca ttaacgccta gatgctgcgc gaatttatta cgattttcac ttagcaactt
     4921 cagtgactct atatccaaag cttgaagtgt caaacgaggc gttgtaacta ttatttgccg
     4981 tgacattgtt tatatttttt tccacttccg caaggacacg ggtcgttgcg tccaactttt
     5041 tgttcggcac gtatcggtgt tgttgtcgat tgcacgggtc gttcagcctg ttcttccccg
     5101 ctcccgctaa agcccattcc ttccgatgat gcatgcacag ccttcatagc acgtgcgcgg
     5161 cgaatcattt gttgttccat ctctgcagcc ggttctgcac gaaaaacaaa ttccacaatt
     5221 tcggatgtga tgcggtcaag caattgcatg aacatctcaa aaccttcggt tttgtattcg
     5281 atcagcggat ctttctgacc ataaccgcgt aagtggatac cttctttaaa ctcttccatt
     5341 tcacgtagat gctctttcca acgttcatca atggtcttga gaacggcgat acgctcgata
     5401 cgacgcatgt tttcagatga ccatacttct tcgcgctgtt gataacgctc catgagcata
     5461 ccgcgaatgt gaacggaaaa ctgctcggca tttaatttat aaaactcatc ttgtgcaacg
     5521 gcaataccac aggaacgcaa cagtgtctcc gtcaatccaa ccaaatccca atcctcggga
     5581 tgcggttgat ttttgacaaa gcgatcattg agcatttcta caaattcgtc cagaacatca
     5641 aggaactcta ctttgatgtt ttcacccatc agtgcacggc ggcggcgact ataaatgatt
     5701 tcacgctgtt gattcatcac gtcatcgtat tcgatgaggc gtttacgaat gctgaaatta
     5761 cggaactcta cccgcttttg cgctctttcg accgctttgg ttatccagcg gtgtgatatc
     5821 acttcacctt cttcggcacc caatttgtcc ataacccgcg caatacgatc ggaaccaaaa
     5881 aggcgcatga gatcgtcttc gagggaaata aaaaacaccg agctgcccgg atcgccttga
     5941 cgaccgcttc gcccgcgtaa ctgcaagtca atacgcctgg attcatgcct ttcggtaccg
     6001 atgatctgca aacctccggc ttctacgaca ccggagccca atttgatatc cgtaccacgg
     6061 cctgccatat tggtagctat cgttacggcg cttttttgtc cggcgttgga tatgatctcc
     6121 gcttcacgct gattttgttt ggcgttgagc acttcgtgtt tgatgcccgt gcgagatagc
     6181 atacggctta aagtctccga aacttcgaca gaggtagtcc cgaccagcgt gggtttaccg
     6241 gctttatggt tttcacgtat cagttcaatg acagcgttgt atttttcccg tttagtgcga
     6301 tagatcatat cttcaaaatc ttcgcgttga atgggtttat tggtcggaat tgccgttaca
     6361 tcaagtttgt aaatttcata aaactcagtt gattccgttg aagccgtacc ggtcataccg
     6421 gctaatttgc tatacaaacg gaaataattt tgaagtgtga tagtcgccaa cgtttgcgta
     6481 tctttttcga cttttacatt ctctttggct tcgatggctt gatgtaaccc atcactataa
     6541 cgtcgtccgt gcaaaatacg gcccgtaaat tcatccacaa tttgcacttt tccgtcttgt
     6601 acgacgtatt cgacatcttt ttcatagagt gagtaggccg tgagcaattt ttcgatactg
     6661 tgaatgcgat cactgcgctc ggcgtacagc gtgccgagtt cttctttttt gattgctttc
     6721 tgatgatcgg ttaattcttt attgccgtca attttgatga tttcctcaga taaatcggga
     6781 atcacaaaca tctcaggatt ggacggtgac agggcgttgc gtcctttttc agtaagatct
     6841 ataccgtgat ttttttcgtc gatagcgaaa aacagctctt catcgatttc gtgcattttt
     6901 ttatcgcgga gatattcgct ttcgacactt tgtatcaatt gcgcaacacc ttcttccgtc
     6961 atggctttca tcaatcgttt gttttttggc gcaccgcgct gtgccatcag cagtttgatg
     7021 ccggcttctt tattttttcc ttcagtgatc agcttatccg cgtcggccac caactggctc
     7081 accaaacgtg tttgcaagtc cacgaggcgc cgtacacccg gattactttc ttcgtatttc
     7141 tgattggtcg gagtatctac agcgccgctg ataatcaaag gcgtacgcgc ttcatctatc
     7201 aacacagaat ccacttcgtc cacgatcgca taatgatgcc cgcgaatctg tacaacgtca
     7261 tcgggtgaag tagccatatt atcacgcaaa taatcaaaac caaattcatt attcgtaccg
     7321 tacgtaatat cacaaccgta ggctacacgg cgttgcgtgg ggttcatatc gttaagaatc
     7381 acgccgatcg taagccccag gaattcataa accttcccca tccattcttt gtcacgcaaa
     7441 gcaaggtaat cgttgacggt aacaaggtgg acgccgtgcc cggccagcgc attcagataa
     7501 accggcgcta ccgccaccag tgttttacct tcaccggttg acatttcgga gattttacct
     7561 tcatgcaata cgatcccgcc gatcatttgt acgtcgtatg gtaccatatc ccactttact
     7621 tcacggccac aaaccatgta tttttgccca accaagcgtt tgcaggcttg ttttacacag
     7681 gcaaaagcct cgggtaaaat ttcatctaaa atatattgtt ccgcttcgcg caaagttttt
     7741 tcggcaactt taagttcttc aaccaaagtt ttagaacgat tcggatcggt attggttcgt
     7801 atcgtttctt tgatcgcctg tacagcggct atttcttctt tcgtattttc tcgtatccga
     7861 tcacgaaact cgttggtttt ggcgcgcaat tcgtcgtccg atagggtgtc gaattgttga
     7921 aagttttgat tgattgcatc cacgatcgga tagagctttt ttacatcgcg ctcgtgtttg
     7981 tttccaaaaa ttttggataa aaggctttga atcatcgtat ctgtatactc cctgtaagtt
     8041 tctttttatc gtccgcgcag aaggcgttcc gccaatatca ccggtaatcc ccgttcgcgg
     8101 gttttgcgtg ccgtggtctg aacatcatac tcactgcgaa tatttttata atcccatttt
     8161 tctgtatcaa acaatccaaa actcaaacgc gcatcgccat cccgcggttg gccgacgctg
     8221 ccgacattga tgatgaactg gaattcgcgg gttatcgccg tcgcatgcgg agaattgcaa
     8281 aacacacgcg gcgaatgcgt atggcctaca aaacatatcc gctcttgaaa tgcatcgaat
     8341 tccggtaagg cgtcattgat cgtaaaaaga tacgtccact tggccggatt catcggcgat
     8401 gcatgcacaa agagtaaatc ctgacgttgg ttgcggtatg gcaatccttg aagaaaggat
     8461 aaactttcgg gactcaactg ctgatgcgtc caccataccg cttgtttggc cacgggatta
     8521 aaattttcgg tagcggacat atcgattacg gcattgtcat gattacccat gatcacggtt
     8581 ttacagtgct tgcgaattaa atccacacac ggttcgggat ccgggccgta gccgacgatg
     8641 tcaccaaggc acacaatttc atcggttccg agttcgccga taatatccaa cgcctgacgc
     8701 aacgcttcta tatttccgtg aatatccgat atgatcgcaa tgcgcatact aatttccgaa
     8761 gctgattccg atttgtcgag ctgattttac cgtttcactg tccgggttaa tataacggat
     8821 tttttcgacg gcattttcta tgctgacgtg aatgatttct ttattacggg cacttgccat
     8881 gtaaccaaac attttttgtt gtgccaattc tacggcatac gcacccatac gcgaggcaaa
     8941 aaggcgatca atgtgcgacg gaggtccacc gcgctgaata tgccccagta caacgcaacg
     9001 cgattcgaca cccgattctt tttcgatctg agcgctgacc cagttaccaa tgcccccaag
     9061 acgctcggcc ccgccgacgt tttgttcggc cgtttcaagt atcatggttt taccgccgac
     9121 cggcattgca ccttcggcta ccatgatgat gctatagttt ttaccttgtt caatacgttt
     9181 ctgtaggtgc gcacacactt tggatatttc aaaagggatt tcaggaatca ggatgacatc
     9241 caccgcaccg gaaataccgg cttctaatgc aatccaaccc gtataacgcc ccatcacttc
     9301 aacgaccata gcacgatgat ggcttttggc tgtagtatgc agtttatcaa gtgcatccac
     9361 ggcgatggta acagcggtat tatatccaat cgttacatcc gtagataaaa gatcatta
//
LOCUS       DAOQNQ010000111         9345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2706_length_9345_cov_6.609042, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000111 DAOQNQ010000000
VERSION     DAOQNQ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9345
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2706_length_9345_cov_6.609042"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PK754_08240"
     CDS             complement(<1..789)
                     /locus_tag="PK754_08240"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNB09531.1"
                     /translation="MIFTNFTRSCILRLVCCISISAPFLNAQENDSLQTRIKKNFLVI
                     PALFYTPETKLGYGAIAAIYFRKPFPENAKPTTLVPSVIYTQNKQFTAGLYADHYWDR
                     EKYHGTTEITFLKYPDVLYGVGPDTPDSSLEKFTPRTFKFSLILQKQWKPKFYAGLQY
                     EIDHTRIIERENTGILSNTSTEGSRRGLRSGVGFMANYDTRDNTQYPTSGGNIIVSFV
                     PFQSWLGSDYSYNRIIVDARRYYPFLRNHIAAVQMYFFQETGKPP"
     gene            complement(891..1103)
                     /locus_tag="PK754_08245"
     CDS             complement(891..1103)
                     /locus_tag="PK754_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010579090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09532.1"
                     /translation="MDKFIRRLIPQAVGITCMVIGWWISIVNVGLDKFVSSTIVNKWT
                     MIGLLLIIIGAYIPQIWIFILNKLNK"
     gene            complement(1450..4635)
                     /locus_tag="PK754_08250"
     CDS             complement(1450..4635)
                     /locus_tag="PK754_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNB09533.1"
                     /translation="MSITELAIKRPSLIVVIFGALTVLGIFGYNQLSYELLPKFAPPV
                     VTISTIYPGASPSEVENSVSKPIEEAVSGLDNVSRINSTSQEGFSLVFVRLTQSANID
                     LALQEAQRKVNGILATMPRDVKTPVITKFALDEFPVLRMGATSSMGPREFYQFLKDRV
                     VPRIAKQSGVAQVTLVGGNEREIKINLDAQKIKSYGLSLARVAQTIKASNLDFPTGNI
                     KQEGTQFVVRVAGKFESVEALSLLVVGRSKQGGDIKLSDVAEVHDGQKDMSTLSRING
                     NESIGILVQKQSDANAVRVSELVRKELASMEKDYENVKLKITIAQDGSQFTVDAANAV
                     KFDLMLAIVLVALTMFLFLHSIRNSIIVMIAIPASLVSTFFAMYLLGFTLNLMTLLAL
                     SLVVGILVDDSIVVLENIYHYLEKGLKPRDAAITGRNEIGFAALSITLVDVVVFVPLA
                     MTTGLIGNIMRQFAIVVTVSTMMSLFVSFTVTPLLASRFSKLERMTNGTFLGRFALWF
                     EALYEAFKNHYLRLLRWALSHRKTVVTATVLLFFSSCTLVPFGFIGNEFVTQGDRGEF
                     AVFLELPSGATIESTNHATQQAEKIITVMPEVSKVFTNVGASSDGFLTMSSNNISELN
                     VTLVDKSERTKTTDEVGALIKARIAEIPGVKVRVNPIGIFGTANQTPIQIVVSATNEA
                     DMKHAADRAMQILAAVPGATDVKLSAEDGKPETRIEVDRQKMAAFGLTIADIGQTLRI
                     ALTGDEDSKFRDGANEYTIRIRLDEFDRSNLNDVRHLSFINSRNEQIELQQFASIYQT
                     AGPSKLQRQDRFGSITVMGQVLGRPSGNVVQDFQKAIALEKDKLGKDVTFTYLGDEEN
                     RSEGFGSMGLAFLAGVLFVYLIMVALYDSYLYPFVVLFSIPVAGVGALLALALAIKSL
                     SIFSLLGMIMMIGLVAKNAILLVDRTNDMKAQGMQTYDALMEAGSARLRPILMTTMAM
                     VIGMLPIALASGAGAEWKTGLAWALIGGLTSSMFLTLIFVPIVYLKFDDWKNRVPQFF
                     KRVTGKQSEPVILATGSNGHYGQAETVKSEA"
     gene            complement(4692..5744)
                     /locus_tag="PK754_08255"
     CDS             complement(4692..5744)
                     /locus_tag="PK754_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB09534.1"
                     /translation="MRKLKITLGIFAVIAFIALGLWNNKVRSASKMNASIAITSVSVS
                     VAPAVMEKIDQAISVVGTITANNDVAIVSETQGRVVSVNAKVGDQLGAGSVIAQVDDE
                     LKKANYLLAEANYQKTKKDLERNEELFKNNAISTSQIESSRLAFQSAEAQYIVARRQY
                     NDTRITTPIGGIVSSRSVDFGTMVVPGTVIANVVDVSKLKVKIQLAEHDVFKIKNGDK
                     AVVSTDVYPGMEFEARVENISAKADEAHTYPVELSLNNNSKNPLKAGMFARVDFKTFV
                     ERTSVVIAREALVGSLKKPQVFVVEGDKAVLRNIVVGVTSSHKIEVLSGLKEGENVVI
                     KGQNNLQDQSTIRVAQ"
     gene            complement(5782..7143)
                     /locus_tag="PK754_08260"
     CDS             complement(5782..7143)
                     /locus_tag="PK754_08260"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNB09535.1"
                     /translation="MKSNRIFKIVLILSFLFSLKIIFAQSQRALTEEEAVKMAVETSK
                     QLRIVEMKAQGASARAKEMRTYRLPSAKLTAGYTRLSEVPDGEFSIPANAFGPGIPSR
                     DIVSKVAPAILNSYLLKGTIQYNVFTGMKLESNEKAARLSEEAAVLDFSKEKKDIVFN
                     TRTAYWNLFKAIEYNKVVDENLEQVKAHLKDAQNLYAQGMITNNDVLKVQVQLSNVQL
                     LQIDAKNAVKLAMVHLNNLIGQSLDTEIQIVSKIQNTDRTFSNYDSLLYKGWTNREDV
                     KAMELRIQAGEASVRAAKGSYMPQVSLQGNVYYNRPNQRIFPSEDAFKYTWDVGLNAT
                     FDLWNWNATAHQVQQAKAVVEQTKDALGLIKDGISLEVTQSYLAMQQAKEKISVTRQT
                     VEQSEESYRITSEKFKKGVALTTDLLDAEVSVLQAKTAYTNAIVDFELAQARLQKAIG
                     QNE"
     gene            complement(7147..7794)
                     /locus_tag="PK754_08265"
     CDS             complement(7147..7794)
                     /locus_tag="PK754_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNB09536.1"
                     /translation="MPEDFEIRTRIVKAALELFVKFGYSKVTMDEIAENLGMSKKTLY
                     QYFPSKQELIQGIIQSAMHEYDTFCDRIIGDQTMRFEDKLRQMLTYFLRVNGNLSREL
                     VADLRKHLPDMWQQIHEWRHTRIAAQFGLVLREGISKGSIRNDIDEKLIIMMYLGIIE
                     NMMNPQVIMQLSQSAPQIFESIWKVFFEGILTADARKTFSVSATVALPTITPQGV"
     gene            8076..8489
                     /locus_tag="PK754_08270"
     CDS             8076..8489
                     /locus_tag="PK754_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB09537.1"
                     /translation="MKRVTGLGGVFFRCEDPGSTMAWYQKHLGVQPDGYGGSFFEWLE
                     KDNTEEIGITAFSTMSKKTKYFHPSRAQFMINFRVKNLKKLMEVLRAEGVQQIGKIET
                     FEYGSFGWILDPNGVKIELWEPVKGYRFKRKRTKK"
     gene            complement(8720..>9345)
                     /locus_tag="PK754_08275"
     CDS             complement(8720..>9345)
                     /locus_tag="PK754_08275"
                     /inference="COORDINATES: protein motif:HMM:NF024352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HNB09538.1"
                     /translation="KKESFLSNKKLDKNYKKVLDGKDINYYSTNWNNKFVRYGNWLHR
                     KRDEKYFLNHKVMIRQIGAVPVATFDDQGFYTLNTIYNLVSVRDYSPKFFLGIINSKF
                     GKWFWMLKNSDFKSLFPKIKKTQIESIPICIINFSNPTEKAQHDKIVTLVEQMLAAHK
                     ALSTVQSPAEKEKLERQIEITDKSIDSLVYELYGLNEEEIKIVEGSK"
BASE COUNT         2487 a         2039 c         2147 g         2672 t
ORIGIN      
        1 aggcggttta ccggtctctt gaaaaaaata catttgaacg gcggcgatat gatttcgtaa
       61 gaaaggataa tagcgtcgtg cgtcaacaat gatccggttg taactgtaat cactgccgag
      121 ccatgattga aacggtacaa atgaaacgat aatattgccg ccggaggtag gatattgcgt
      181 attgtctctg gtgtcgtagt tggccataaa accaactccc gagcgcaatc cgcggcgtga
      241 accttccgtt gaggtgttgg ataaaatccc ggtattctcg cgttcgataa tacgtgtatg
      301 gtcgatttcg tattgtaaac ctgcgtagaa cttaggtttc cactgttttt ggagaataag
      361 cgaaaacttg aatgtgcgcg gcgtaaattt ttccaatgac gaatcgggtg tatcgggacc
      421 gacaccatag agtacatccg ggtatttgag aaatgtaatt tctgtagtgc cgtgatattt
      481 ttcgcgatcc caataatgat cggcatacaa tccggcggtg aattgtttgt tttgtgtata
      541 tataaccgac ggtactagcg ttgtaggttt ggcattttca ggaaacggtt tacgaaaata
      601 aatagccgca atggcgccat agcctaactt tgtttcaggt gtgtaaaaca gagcggggat
      661 gacaagaaaa ttttttttga tgcgggtttg taacgaatca ttttcttgtg cgtttaagaa
      721 cggggccgat atagagatac agcacacgag ccgcagaata caagagcgcg tgaagtttgt
      781 gaagatcata gttgcaatgt tttaaaaacg aaaaaggggt agttccttgc gaaacggccc
      841 ctttttgctg tgaacgctcg aagtttcctc cgacacctcc acatagacgg ttatttattt
      901 aatttattca aaataaaaat ccagatttgc gggatatacg cgccgatgat gatcagcagt
      961 aagccgatca tcgtccattt attgacgatc gtactggaaa caaatttatc caatccgaca
     1021 ttaacgatac tgatccacca accgatgacc atgcatgtta tcccaaccgc ttgcgggatg
     1081 agacggcgaa taaatttatc catgtacggc ctgctttaaa ttagaaaaaa cgatgttatc
     1141 cttcgtgtcg ttgtttgttg tctggcagga ctcgccttca cagcagcgga tttgaacatt
     1201 gtgacagcgc cggcattggt aatgtccatg cacaaacaca aaaccttccg cactgccgca
     1261 gaaaaaacac tgtgatgaag acaccataga tatagtaaaa tgaaatggcg agtaccgtat
     1321 gaagtaaatt aaacgggcga tggatatatt gcaaggataa aaaagtaaaa taaaaacggt
     1381 tacaccgatt gaatgtgtaa ccgtttgtaa acgatatgaa tttgtaattc aaaaaactac
     1441 cgcatgatcc tacgcctctg attttaccgt ttcggcttgt ccataatgac cgttggagcc
     1501 ggtagccagt atgacgggtt cgctctgttt acccgtaacg cgtttgaaaa actgaggaac
     1561 tctgtttttc caatcatcaa acttcagata cacgatcggc acaaaaatca acgtgaggaa
     1621 catcgaactt gtcaaaccgc cgatcaaagc ccaggcgaga cctgttttcc attccgcacc
     1681 ggcgccgctg gctaaggcaa taggcaacat accgatcacc atcgccatcg tcgtcatcag
     1741 aatggggcgg agtcgtgcgc tgccggcttc catcaaagca tcataggttt gcataccttg
     1801 cgctttcatg tcattggtac gatcaacaag caagatggcg tttttcgcaa ccagaccgat
     1861 catcatgatc ataccgagta atgaaaagat cgagagcgac ttgatggcta aggcaagcgc
     1921 aagcaatgcg cccacgccgg ccacggggat tgaaaataaa accacaaacg gatacaggta
     1981 tgaatcgtac agcgccacca tgataagata aacgaaaagt acgccggcaa gaaaagccaa
     2041 tcccatactg ccgaaacctt ccgatcggtt ttcttcatcg ccgagataag tgaatgtaac
     2101 atccttaccg agtttatctt tttccaaagc gatggctttt tggaaatcct gcaccacatt
     2161 accgctcggg cgtccgagta cctgtcccat cacggttata gaaccgaaac gatcctgacg
     2221 ttgcaatttg gaagggccgg ccgtttgata aatcgatgca aattgttgga gttcgatttg
     2281 ttcgttacgg ctattgataa aactcagatg gcgcacgtcg ttgagattag aacgatcaaa
     2341 ttcgtcgagg cgaatgcgta tcgtatattc gttggcgccg tcgcgaaatt tggaatcttc
     2401 atcgcccgtc aaagcaatgc gcagcgtttg tccgatatcg gcgatggtca gaccgaaagc
     2461 tgccattttt tgacgatcta cttcgatacg cgtttcgggt ttgccgtctt cggctgaaag
     2521 tttgacgtcg gttgcgccgg gtaccgcggc caaaatttgc atcgcgcggt ccgcggcgtg
     2581 tttcatgtcg gcttcattgg ttgcgctgac cacgatttgt atcggggttt gattggccgt
     2641 accaaaaata ccgatcggat ttacgcgcac tttaactccc ggaatttcgg cgatgcgtgc
     2701 tttgatcaac gctccgactt catccgtcgt tttggtgcgc tcggatttat ccaccagcgt
     2761 aacattcaat tcggaaatat tattcgacga catcgtaaga aacccgtcgc tggatgcgcc
     2821 gacattggta aatactttac tcacttcagg catcacggta atgatctttt cggcctgttg
     2881 tgtcgcatga ttggttgact cgatggtcgc acccgaagga agttcgagga aaacggcgaa
     2941 ttcaccgcga tcgccctggg tgacgaattc attaccgata aatccaaagg gaaccagcgt
     3001 acagctcgag aaaaacaata gtaccgttgc ggtgactacg gttttgcgat gggataaagc
     3061 ccaacgcaac aaacgcaaat aatgattttt aaatgcttca tagagggctt caaaccataa
     3121 tgcaaatcga cctaaaaaag tgccgttcgt cattctttcc agtttggaaa aacgcgaagc
     3181 gagaagcgga gttactgtaa atgaaacaaa caaactcatc atcgtggaaa ccgtaactac
     3241 gatggcgaac tggcgcatga tgtttccgat aaggcctgtg gtcatggcca gcggcacaaa
     3301 tacgaccaca tccacgagcg tgatggataa cgccgcgaaa ccgatttcgt tacgtccggt
     3361 gatcgctgca tcgcgcggtt tcaagccttt ttccaaataa tgataaatat tttcaagcac
     3421 gacgatcgag tcgtccacga gaatacctac gactaacgac aacgcgagca aggtcatcag
     3481 gttgagagta aatcccagca ggtacatggc aaaaaaagta gaaacaagcg acgccgggat
     3541 cgcgatcatc acgatgattg aatttcgaat gctgtgcaaa aacaaaaaca tggtcaacgc
     3601 aaccagtacg atggccagca tcaaatcaaa tttgaccgca tttgccgcat ccacggtaaa
     3661 ttgggaaccg tcttgagcga tcgtaatttt taattttacg ttttcataat ccttttccat
     3721 gctcgcgagc tctttacgaa caagttcact tacgcgcacg gcattggcat cggattgttt
     3781 ttgaaccaaa ataccgatgg attcgtttcc gttaattcgg ctcaaggttg acatgtcttt
     3841 ttgaccatca tgcacttcag cgacatcgga taacttgata tctccgcctt gtttggagcg
     3901 tccgacgaca agcaaactta acgcttcgac ggattcaaac tttcccgcaa cgcgaacgac
     3961 aaactgggtg ccttcttgtt tgatattgcc ggtcggaaaa tcaaggttag aggctttgat
     4021 cgtttgggct acgcgtgcca gtgataggcc gtaagatttg attttttgcg catccagatt
     4081 gattttgatt tcgcgctcgt taccgccgac caaagtgacc tgtgcgacac cggactgctt
     4141 ggcgatgcgc ggtacgacgc ggtctttgag aaattgataa aattcacgcg gacccatcga
     4201 actggtcgca cccatacgca ataccgggaa ttcgtccaat gcaaatttgg taatgaccgg
     4261 tgttttgacg tcgcggggca tggtcgcaag gataccgttg actttgcgct gcgcttcctg
     4321 caatgcaaga tcaatattgg cagactgggt caaacgaaca aataccagcg aaaaaccttc
     4381 ctgggaagtc gaattgatac gggagacatt atccagaccg gagacggctt cttcgatcgg
     4441 tttactgacg gagttttcca cttcggatgg agaagcgccg gggtagatcg tactgatggt
     4501 gactaccggt ggtgcaaatt ttggcaacag ttcataactg agctgattgt atccgaaaat
     4561 accaagcacc gtaagcgcac cgaagatcac gacgatcagg gacggacgct ttatggctaa
     4621 ttctgttata ctcatttttt atttttcctg cttcgttgga tagcgttaca tattaaaaca
     4681 ataccataat actactgggc gacacggatg gtggattgat cctggagatt gttttgtcct
     4741 ttgatcacca cgttttcgcc ttctttgaga ccgctcaata cttcgatttt gtgactggat
     4801 gtaacgccta ccacaatatt gcgcagcacg gctttatccc cttcgactac gaatacttgc
     4861 ggttttttca agctgccgac taaggcttcg cgtgcgatga ctacggatgt acgttctaca
     4921 aatgttttaa agtcaacacg ggcaaacatt ccggctttga gcggattttt ggaattatta
     4981 ttgaggctca gttcgaccgg ataggtgtgc gcttcatccg ctttggcgct gatgttttct
     5041 acacgggctt caaattccat cccgggataa acatcggtgg agaccacagc tttatcgccg
     5101 tttttgattt taaatacgtc gtgctcggcc aattggattt ttactttcag tttcgaaaca
     5161 tccacgacat tggcaatcac ggtgccggga acgaccatcg ttccgaaatc aaccgaacgg
     5221 gacgaaacga tgccgccgat cggagtcgtg atacgcgtat cattgtactg acggcgtgcc
     5281 acgatgtatt gcgcttcggc cgattggaaa gccaggcgcg aagattcgat ctggctggtc
     5341 gagatcgcgt tgtttttaaa aagttcttcg ttgcgctcga ggtctttttt tgttttttga
     5401 tagtttgctt cggcaagcag ataattggct tttttcagtt cgtcatccac ctgtgcgatg
     5461 accgatccgg cgcctaattg atcgccgact ttggcattga cgctcaccac acgaccttgc
     5521 gtttccgata cgatggccac gtcattattg gccgtgatcg taccgacgac ggatatcgct
     5581 tgatcgattt tttccatgac cgccggtgcg acggatacgg aaaccgatgt gatcgcgatc
     5641 gaagcattca ttttggaagc gctgcgcact ttgttattcc acaaaccgag cgcaataaaa
     5701 gctatgaccg caaaaatccc gagtgttatt tttagtttac gcatgatata aaactccatt
     5761 ttatagtttt ttatttaaaa attattcgtt ttgtccgatg gctttttgca gacgcgcttg
     5821 agccaattca aaatccacga tcgcattggt ataagccgtt ttggcttgaa gcacggaaac
     5881 ttcggcatct aataaatcgg tggtcagagc gacgcctttt ttaaattttt cggaagtaat
     5941 acgatagctt tcttccgact gctctaccgt ctgacgggta acggaaattt tttctttagc
     6001 ttgttgcatc gccagatagc tttgggtcac ttccagactg ataccgtctt tgataaggcc
     6061 gagcgcatct ttggtttgtt caacgaccgc tttggcttgc tgcacctgat gtgccgtcgc
     6121 attccagttc cacaaatcaa aagtggcatt cagacccaca tcccaggtgt acttaaaagc
     6181 atcttcagag ggaaaaatac gttgattggg tcggttataa tacacgttgc cttgcagcga
     6241 tacctgcggc atgtagctac ctttggctgc acggacggac gcttcaccgg cttgaatacg
     6301 caattccata gcttttacat cttcacggtt ggtccaacct ttatagagca gggaatcata
     6361 gtttgaaaag gtacgatccg tgttttgtat tttggaaacg atttggatct ctgtgtcgag
     6421 cgattggccg atcagattat tgagatgaac catcgccagc ttgaccgcat ttttggcatc
     6481 aatctgtaaa agctgaacat tggaaagctg tacctgcact ttgagcacat cgttattggt
     6541 gatcatacct tgcgcataaa gattttgggc atctttaaga tgggctttga cctgttcgag
     6601 gttttcatcc acgactttgt tgtattcgat cgctttaaac agattccaat acgccgtgcg
     6661 cgtattaaat acgatatctt ttttctcttt gctaaaatcc aaaaccgccg cttcttcgga
     6721 gaggcgtgca gccttttcat tggattcgag tttcatgccc gtgaatacat tgtattgtat
     6781 tgtacctttg aggaggtagc tgttaagaat agccggcgct actttggaaa cgatatctct
     6841 ggacggtata ccggggccaa aagcattagc cggaattgaa aattcgccgt ccggtacttc
     6901 actcaaacgc gtatatccgg ccgtcagttt ggcagacggt aagcggtagg tacgcatttc
     6961 tttagcgcgg gccgaagcgc cttgcgcttt catttcgacg atgcgcagtt gtttgctggt
     7021 ttcaaccgcc atttttacgg cctcttcttc ggtcagggcg cgttgcgatt gggcaaaaat
     7081 aatttttaga ctaaaaagaa aactcaaaat gagcacgatc ttgaatatcc ggttgctttt
     7141 catatatcaa actccttgtg gcgtaatcgt gggtaaagcg acggtggcgc tgacggaaaa
     7201 tgttttacgg gcgtcggcgg taaggatgcc ttcgaaaaat actttccata tactttcaaa
     7261 aatctgcggc gccgactgag acaactgcat gataacttgc ggattcatca tgttttcgat
     7321 aatgccgagg tacatcatga tgatcagttt ttcatcgata tcgttgcgta tggaaccttt
     7381 actgatgcct tcacgcaaaa caagaccaaa ctgtgccgcg atccgcgtgt ggcgccattc
     7441 gtgtatctgt tgccacatgt cgggcaagtg tttgcgcaga tcggccacga gttcacggct
     7501 aaggttaccg ttgacacgca aaaaatacgt cagcatttgt ctcaacttat cttcaaaacg
     7561 catcgtttga tcgccgatga tacgatcaca aaacgtatcg tattcgtgca tggccgactg
     7621 tataatgccc tgtatcaact cttgtttact tgggaaatac tggtacaagg tttttttgct
     7681 catgccgagg ttttcagcga tttcgtccat agtgactttg ctgtaaccaa atttgacaaa
     7741 aagctccaaa gcggctttta cgatgcgggt tctgatttca aaatcttcgg gcacttttgg
     7801 actccttaaa ataaatttta atgacccatt caaacgtgtg gaaactcaaa aagtttcatt
     7861 agtttccatt ttttaaaaaa aattagccga tgtttaatct agctaaatca attacttaat
     7921 catttttcaa gaaaactata tttgttttct tggtttccaa tatacaaaaa aagtgacttt
     7981 tgtcaagtaa ttctttcatt tttgttgaac ttgggtatcg tacgtgatac tttcgaagta
     8041 cctttatagc ccaccccata ttcaaggagg tttttatgaa acgcgttacc ggtttaggcg
     8101 gagttttttt tcgttgcgaa gatccgggtt cgaccatggc ttggtatcaa aagcatctcg
     8161 gggttcaacc cgacggatac ggcggcagtt tttttgagtg gctggaaaaa gataatacgg
     8221 aggagatcgg catcacggcg tttagcacga tgagtaaaaa aacaaaatat tttcatccga
     8281 gccgcgctca gtttatgatc aattttcgcg tgaagaatct caagaaactg atggaagtat
     8341 taagggcgga aggagttcag caaatcggta agatcgaaac ttttgaatac ggttcttttg
     8401 gctggatact cgatcccaat ggcgtaaaaa tcgaattgtg ggaaccggtc aaagggtatc
     8461 gctttaaacg caaacgaaca aaaaaataga atgtgatatt atggtgtagt taatgattgc
     8521 gggtattcct caaggctctt gggagatttt ggtgttggga taagagtttg cggagttaag
     8581 taacgtttgc gttacttatg tggattcagt ttgggggcag gaaaaatcca aagttagaaa
     8641 aatatccgaa aatgcgacag aatcctacac gtcaggtgca cactttgttg gtaagttttt
     8701 ttggaagact ccatgcctat tacttggaac cttccacaat tttgatttct tcttcattca
     8761 acccgtataa ttcataaacg agtgaatcaa ttgacttgtc tgtgatttcg atttgacgtt
     8821 ccaatttttc cttttcagcg ggactttgca cggtggacag ggctttgtga gccgcaagca
     8881 tttgctctac aagggttact attttgtcgt gttgcgcttt ttcggtgggg ttggaaaagt
     8941 taataataca aattggaata ctctcaattt gcgttttctt tatttttgga aacaatgact
     9001 tgaaatcgct gtttttcaac atccaaaacc attttccaaa tttgctgtta attattccta
     9061 aaaagaattt tggtgaataa tctcttacgc taactaaatt gtagatggta ttaagtgtat
     9121 aaaaaccctg atcgtcaaat gttgcaacag gtacagcgcc aatttgccga atcatgacct
     9181 tgtggttcaa gaaatacttt tcatcacgct ttctgtgcaa ccagtttccg tatcgcacaa
     9241 atttattatt ccaattagtt gagtaataat taatatcttt accatccaag acctttttgt
     9301 aatttttatc cagttttttg ttagacagga aactttcttt cttgt
//
LOCUS       DAOQNQ010000112         9100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2802_length_9100_cov_5.996241, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000112 DAOQNQ010000000
VERSION     DAOQNQ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9100
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2802_length_9100_cov_5.996241"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PK754_08280"
     CDS             complement(<1..555)
                     /locus_tag="PK754_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09539.1"
                     /translation="MSLNLLKNSTAFLYVTFFLVLIDVKAQQEDLLLLKAKDHAMQYY
                     VSLPKGWSNTKTWPVLVVCEAAEKQYKENALRFIKARGERPYIIVAPFIVTNGNQGLR
                     NPEIFPYTSDTWDQIDREGTCTFDLQGLDAVLKDVKKKYNGEARIHITGFEAGAHLAW
                     AMTFLQPEKIKSIVVVAGNFRSRCL"
     gene            complement(663..2501)
                     /locus_tag="PK754_08285"
     CDS             complement(663..2501)
                     /locus_tag="PK754_08285"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HNB09540.1"
                     /translation="MKILSAIASIIITAGSVLAGDKLDLRYDVDLTKIEKDSFFVTLH
                     VKGIQNDTTVFQFASTAPGTYQVMDIGRFVGGFTAYDEKGRVLPVIHRSVNQYVIAGA
                     KKLHTIRYQVEDSYDTIIKEDQVGPMSGSSIETDNVLLNGQMVFGYFQGFQSNPMQIK
                     YNYPQGWLVGTALEEKNGTYKAKTFDEVVDSPFLFGKLTKAGMKIGGAQVDVFCHAKN
                     GTMAADSLLSHLKDILISAEQFLGGLPVKRYAFLYKFTSDPLPAWGAWEHSYSSFYIL
                     PESISFPSGGGMGSGLADIIRSTAAHEFFHIVTPLNIHSEIIERFNFEQPTPSRHLWF
                     YEGVTEWAAQIMQVRSGLVQPNEFCERVSQKMSVNDNYDPRVSLVDLSLGSYGHLNAQ
                     YQNIYAKGALTAMLLDMRLLELSKGKTGLRDVVLKLSKKYGPDKAFPDSEFFEINVKM
                     TYPEIDDFIKRYIQGNEPLPVSEYIAKAGYDYVKSKPTGRFRSVRGTFSLSLDHDKVV
                     VSGVDTTDEVNRQTGIREGDVILGMWYQDKLYDLRDPAMQQIRQTMQPGESFAWKIER
                     DTKEMMLQGTAGKIEIMENHVITPVSEPSAEQQQFHSWWLKPFKGK"
     gene            complement(2524..4323)
                     /locus_tag="PK754_08290"
     CDS             complement(2524..4323)
                     /locus_tag="PK754_08290"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HNB09541.1"
                     /translation="MRWMKLWVMAVTFSGSLIAGDIKLNYRVDISEPMQDRFYVTLVV
                     KGWSNDSAVFQFASTAPGTYQVMDVGRFVNGFTASDSKGKSLPVVQRGINQYVINDAK
                     NLATISYRVEDVNDTNLAVHPIYPMAGSNIEKDNALINGQMVFGYFHGYQANPIQVQI
                     DCPKDWKVATALTPKNGLYTADSFDHIVDSPFLLGRLTEVKTKVGGAEVGIYCYSQNN
                     IVTADSLLEALQAMLVAAEKFLGELPVKRYAFLYHFRQDVPAAYGAWEHSYSSEYVMP
                     EKPMSVYGETVKDFSAHEFFHIVTPLNIHSEIIQQFNFVKPTPSDHLWLYEATTEWAS
                     HLMQVRGGMITSDEFKERITNKLVTSDYFRSDLSLVDLARGSFGDLAPQYGNIYIRGA
                     LTMMLFDMRLLELTKGKMGLNDLVKKLAKQYGPKKAFPEKEIFDIVVKMTHPDMKDFI
                     DRYIKGYEPLPVESMLNLAGYAYHAKFETNEEKTDMGKFDFDYGTDKITFKDVDASNA
                     VTAKLGLQNGDAFLGFIKDGTEFRLGKPGVQKAMNSVKPGDEFGLIVQRGSEEVKLTA
                     KAGVKKNIKPHVILPVEKPTSDQLAFRKRWLSN"
     gene            complement(4422..6230)
                     /locus_tag="PK754_08295"
     CDS             complement(4422..6230)
                     /locus_tag="PK754_08295"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09542.1"
                     /translation="MTRRAFFLWIIFAHKAVSAFAQLPELTYRMDLTSLDDDSFFVQL
                     DIKKYPHSSALFQFPMTAPGTYAILDAGRFVGSFSALDTSGHELTVTRTSVNQFRIDE
                     ARRLARIVYKVNDSFGERIGENPIHPMGGTNIEPDNIVINGFMTFGYFSEFQNIPYRL
                     RVTMPADWKLGTALVSKDGYLIAPTYDDLVDAPLMAGRLTTAHWTMDKTKISVYCYSE
                     KNRVNATWLRSQLKDILNASKKFIGTLPVDRYVFLFHFREDTGPIYGALEHNYSSFYV
                     IPEMKLEGLRPIVQSIAAHEFFHIITPLNLHSEKIVPYRFDRPTPSRHLWLYEGVTEW
                     ASDIMQVRAGVIPDSVYMQRLREKLQRNDRYKPDISLETMSLGAYDVYEDQYANIYER
                     GALTAALLDMKLLELSDGKRGLRDVIMALVKKYGAGKSFTDSSLYGIIVDHTYPEVRE
                     FMDRYIRGSERLPIAEYLIKAGYQYESKYETTRMRNHLGLFEVMQKDTMVIVTSVDPN
                     DSVCTRLGLQAGDIIEKMVLNNIDLPLTDVSLIEFFSFIDEGASFAWVVRRGSQTLTL
                     NALAGRAPVIEKHRIRPISDPTPEQLRFRRIWLHIP"
     gene            complement(6227..6553)
                     /locus_tag="PK754_08300"
     CDS             complement(6227..6553)
                     /locus_tag="PK754_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HNB09543.1"
                     /translation="MISYDDFDKVEIRTGTIIEVNDFHKARKPAYQLTIDFGVAGIKR
                     SSAQITDMYDKQALIGKQILAVVNFPPKQIANFFSECLVLGVVTPEGVVLLQTERPVA
                     NGLRIS"
     gene            complement(6550..7308)
                     /locus_tag="PK754_08305"
     CDS             complement(6550..7308)
                     /locus_tag="PK754_08305"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNB09544.1"
                     /translation="MSTRLGIDVGNTHIKIGVLDGERVVDVWRFRTDSERSVDEYYFQ
                     LQNVCREAKLDTIPELLIASVVPSVTIAFQLLEETRGVKSSFIDVKSPLSYGFHPAIN
                     HQVGADILILAEAAVRLKGKDVIIVSAGTATTVFAVSDGVFQGGAISPGIKGSVESLI
                     AKAALLKTVYLDVPNSPIGQNTDDAVRSGVIFGYAGMIDGLVTRMREQLGKPDMPVLA
                     SGGMIAKFAKVSRTIHEIKPDLGIQGLAYLSGKL"
     gene            complement(7305..7862)
                     /locus_tag="PK754_08310"
     CDS             complement(7305..7862)
                     /locus_tag="PK754_08310"
                     /inference="COORDINATES: protein motif:HMM:NF016144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HNB09545.1"
                     /translation="MKIHVIAYDAQWAVLFESESRRLTKFWPDKTLSIEHIGSTAVPG
                     LAAKPVVDILIGTDDMIELQAMVSKLTDADYRYIPEYEKYIPERRFFIRPKNNVEEKS
                     INWNPDYGARDHLFHIHAAVRTSPFWGRHITFRDVLRRDKALRDAYADLKNKLSENVP
                     EDKNTYAQGKFEFIQAVGKKLGVQV"
     gene            7965..8393
                     /gene="aroQ"
                     /locus_tag="PK754_08315"
     CDS             7965..8393
                     /gene="aroQ"
                     /locus_tag="PK754_08315"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010274081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNB09546.1"
                     /translation="MKFLILNGPNLNLLGEREPHIYGTQTLADIEDNLKTVFPEHTLE
                     FKQSNMEGELIDLLHAVRKTHNGVVFNPGAYSHYSIALQDAITSIQIPVVEVHLSNIH
                     AREEFRSRSVVARACIGQVSGFGSYGYEMAIRALIQHHTK"
     gene            complement(8422..8886)
                     /locus_tag="PK754_08320"
     CDS             complement(8422..8886)
                     /locus_tag="PK754_08320"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNB09547.1"
                     /translation="MEAVAPKPKYKTFTYHTGLHRIATRAAMLSSEDKPAFRVASPPE
                     FKGETDVWTPEDLFVAAAEICTMTTFISFTEKAGLHIADYQSRANGILEFVEGGYRFT
                     KIIVRPKIAVDNEADLEKARSVMHDAHKKCLIANSMKTEVIVEAEFVITETA"
BASE COUNT         2539 a         2135 c         1829 g         2597 t
ORIGIN      
        1 caggcaacgg ctcctaaaat taccggcaac gacaacgatg gatttgattt tttcaggctg
       61 aagaaaagtc atcgcccacg ccaaatgagc gccggcttca aagcccgtaa tatgtatgcg
      121 cgcttcgccg ttgtattttt tttttacatc tttcaatacg gcatcgagac cttgcaagtc
      181 aaaagtgcaa gttccttcgc gatcaatttg atcccatgtg tctgatgtat aaggaaatat
      241 ttcaggatta cgcagaccct gattcccatt cgttacgata aacggtgcca cgatgatgta
      301 aggtcgttcg ccgcgcgctt ttatgaaacg aagtgcattt tctttgtact gtttttctgc
      361 ggcttcgcag actaccagta ctggccacgt ttttgtatta ctccaccctt tgggcaatga
      421 tacataatac tgcattgcat gatctttagc tttcaagagc aaaagatctt cttgctgagc
      481 cttgacatca atcaaaacta aaaagaaagt tacatacaga aatgcagtcg aattttttaa
      541 aagatttaaa ctcatatatt tcattttata ataaacacta ccccgccaag tttgacgggg
      601 tagctcgttt aaaaaaaaat cgaaaggaca aatcattaaa tccttacata tctgtcaaag
      661 cgctattttc ctttaaaagg cttcaaccac cacgaatgaa attgttgttg ttccgctgag
      721 ggttcactaa caggagttat tacatgattt tccatgattt caattttacc ggctgtacct
      781 tgcaacatca tttctttggt atcgcgctcg attttccacg caaaggattc gccgggttgc
      841 atggtttggc gaatttgctg catcgcagga tcgcgcaaat cataaagctt atcctgatac
      901 cacatcccca atatcacatc tccttcgcgt attcccgttt ggcgattaac ttcatcggtc
      961 gtatcaacac cgctaaccac aactttatcg tggtctaacg acaacgagaa ggtacctcgc
     1021 acggaacgaa atctacccgt aggttttgat ttaacgtaat catatcccgc ttttgcaatg
     1081 tattcactca ccggtagcgg ctcgttaccc tggatatatc gtttaataaa atcatcgatt
     1141 tcagggtacg tcatcttgac gttgatttca aaaaattctg aatccggaaa agctttatcc
     1201 ggtccgtatt ttttggaaag cttcaatacg acatcgcgca atcccgtttt acccttcgaa
     1261 agctctaaca atcgcatatc gagtaacatg gccgtcaaag cgcctttggc atagatattt
     1321 tgatactgcg cgtttaagtg cccatacgaa cctaaggata gatccaccaa gctgacgcgc
     1381 ggatcataat tgtcgttgac actcattttt tgagaaactc tctcacagaa ctcatttggt
     1441 tgaactaatc cgctgcgcac ttgcatgatt tgtgcagccc attccgtaac cccttcatag
     1501 aaccacaaat ggcgtgatgg tgtgggttgt tcaaaattaa atcgttcgat gatttcgctg
     1561 tgaatgttaa gcggcgtgac aatgtggaaa aattcatgcg ccgccgtact tctaatgata
     1621 tccgcaagcc cggaccccat gcctccgcct gaaggaaaag aaatgctctc cggtagaatg
     1681 taaaacgagg aataactgtg ttcccatgca ccccaagccg gcaaaggatc tgaggtaaac
     1741 ttatataaga aggcataacg tttgaccggc agtcctccta gaaattgctc tgctgatatc
     1801 aaaatatctt taaggtgcga aagcagactg tcagccgcca tcgttccatt tttggcatga
     1861 caaaatacat ccacctgagc tccgccgatt ttcataccgg ctttagtcag cttaccaaac
     1921 aaaaacggag agtcaaccac ttcatcgaaa gttttagctt tataggttcc gtttttttct
     1981 tcaagcgctg taccgaccag ccatccttgg ggataattgt atttgatctg catcggattt
     2041 gactgaaagc cctgaaaata tccgaaaacc atctgaccgt tgagtaaaac attatccgtt
     2101 tcgatcgaag atccgctcat cggacccact tgatcttctt tgattattgt atcatacgaa
     2161 tcctcgactt gatatcggat cgtatgtagt tttttggcac ccgcgatgac atactgatta
     2221 acgctgcgat gtataaccgg caacacacgc cctttttcat catacgccgt aaaaccgccg
     2281 acaaaacgac cgatatccat tacttggtac gtgccaggtg ccgtcgaggc aaactgaaac
     2341 accgtcgtgt cgttttgaat tcctttaacg tgcaacgtaa caaaaaaaga atctttttct
     2401 attttggtca aatcaacatc gtatcgcaaa tctagtttat ctccggcaag tacggaacct
     2461 gcggttatta taatcgatgc aatggcgctt aatattttca ccttcgtctc ctgattctgt
     2521 agattaatta cttagccaac gtttacgaaa tgccaattgg tccgacgtcg gtttctcgac
     2581 gggaagtatg acatggggct tgatattttt tttgacaccg gccttagctg tcagtttgac
     2641 ctcttcactg ccacgttgta cgattaagcc aaattcatcg ccgggcttaa cactattcat
     2701 tgctttttgt acgcccggtt tgcctaaacg aaattccgta ccatctttaa tgaagcccag
     2761 gaaagcatcg ccgttttgca agcccagttt ggccgtcacg gcattggatg catccacgtc
     2821 tttaaatgtt atcttatccg taccataatc aaaatcaaac ttgcccatgt ccgttttttc
     2881 ttcattcgtt tcaaactttg catgataggc ataacccgcc aaattcagca tactttctac
     2941 gggcaatggt tcgtatcctt tgatataacg atcaataaaa tctttcatgt cggggtgcgt
     3001 cattttgacg acaatgtcaa aaatttcttt ttccggaaat gcttttttcg gtccgtactg
     3061 cttggccagt tttttaacca aatcatttaa acccatttta cccttggtga gttcgagcag
     3121 tcgcatatca aaaagcatca tcgtcagtgc accacggatg taaatattac cgtactgcgg
     3181 tgccagatca ccaaacgatc cgcgtgcaag atccactaaa ctgagatctg aacgaaaata
     3241 atcactggta actaatttat tcgtaatacg ctctttaaat tcatccgaag taatcatgcc
     3301 gccgcgcact tgcatgagat gggaagccca ttccgtcgtt gcctcgtaca gccaaagatg
     3361 atcggaaggc gtcggtttta caaaattgaa ttgctggata atttcactgt gaatattgag
     3421 cggtgttaca atatgaaaaa attcatgcgc ggaaaaatct ttcaccgttt caccataaac
     3481 actcatcggt ttttcaggca taacgtattc tgaggaatag ctatgttccc atgcaccata
     3541 agccgccggt acgtcttgac gaaaatgata taaaaacgca taccgcttta caggcaattc
     3601 acctaaaaat ttttccgcag cgactagcat cgcttgcaat gcttcaagca gcgaatcggc
     3661 cgtaacgata ttattttgcg aataacagta gatacctact tcagcaccac caaccttggt
     3721 tttcacttcg gtcaatcgcc ctaataaaaa cggcgaatct acgatgtgat caaacgaatc
     3781 ggcggtgtat aaaccgtttt tgggagttag cgcggttgcg accttccaat cttttggaca
     3841 gtcaatttgc acttgtatcg gattagcctg atacccgtga aagtatccaa aaaccatctg
     3901 gccgttaata agtgcattgt ctttttcaat attggatccg gccatcggat aaatgggatg
     3961 aacggctaaa ttggtgtcat taacatcttc tacgcgatac gatatcgtgg ccaaattttt
     4021 agcgtcattg attacatatt gattaatgcc gcgttgtacg acaggaagcg attttccttt
     4081 gctgtcgctg gccgtaaaac cattgacaaa gcgaccgaca tccatcacct gatatgtacc
     4141 gggcgctgtc gaagcgaatt gaaataccgc cgaatcattt gaccatcctt ttacaaccag
     4201 cgtcacataa aaccgatctt gcataggttc ggaaatgtcc acacggtaat tcagtttgat
     4261 atcccctgcg ataagcgacc cggaaaaggt aacagccatt acccatagtt tcatccaacg
     4321 catcgaacgt ctccttaata ataaaatgat cagatcgtat caaatgcttg aaatacgtta
     4381 atggttacga aatgtttctt tgttttttta aaataccgga ttcaggggat atggagccaa
     4441 atgcggcgga aacgaagctg ttccggagtg ggatcggata tgggacgaat acgatgtttt
     4501 tcgataacgg gggcacgacc ggctaaggca tttaatgtca acgtttgcga accgcgacga
     4561 acaacccagg caaacgatgc gccttcgtct ataaatgaga aaaattcaat aagcgaaaca
     4621 tcggtcaaag gcagatcaat gttattaagt accatttttt cgatgatgtc gccagcttgc
     4681 aaacccaatc gggtacaaac cgaatcgttg ggatcaacgg aagtaacaat taccatcgtg
     4741 tctttttgca tcacttcaaa aagcccaagg tgattacgca tccgcgttgt ttcatacttg
     4801 gattcgtatt gataacctgc cttgataaga tattccgcta taggtagccg ctccgatccg
     4861 cgaatgtagc gatccatgaa ttcacgcacc tccggatagg tgtgatcaac aatgataccg
     4921 tataaggaac tgtcggtaaa agatttcccc gcaccatatt tttttacaag tgccatgatc
     4981 acatcgcgaa gaccgcgttt accgtcggaa agttccaata atttcatatc caatagtgcg
     5041 gctgtaagcg caccgcgttc gtagatatta gcatattgat cttcatacac atcatacgcg
     5101 ccgaggctca tcgtttcgag actgatatcc ggcttgtatc gatcgttgcg ctgtagtttt
     5161 tcgcgtaagc gctgcatata tacactgtcg gggatgactc cggcgcgtac ctgcatgata
     5221 tcactggccc actcggtaac gccttcataa agccagagat gacgcgaggg tgtcggacga
     5281 tcaaagcgat acgggacgat tttttcacta tgcaaattga gcggcgtgat gatatgaaaa
     5341 aactcatgtg ccgctatgct ttgtacgatg ggccgaaggc cttcgagttt catttccggg
     5401 atgacataaa acgacgaata attatgctcc agcgcgccgt atatcggccc ggtatcttca
     5461 cgaaaatgga ataaaaaaac ataacgatct accggtaatg tgccaataaa tttttttgat
     5521 gcattcagta tatctttgag ttgcgagcgt aaccaggtcg cgttaacacg atttttttca
     5581 gaataacaat aaaccgatat cttggtttta tccattgtcc aatgagccgt cgtaaggcga
     5641 ccggccataa gcggcgcatc taccaaatca tcgtaagtcg gtgctatgag gtaaccgtct
     5701 ttagaaacta acgccgtacc caatttccaa tcagcaggca tcgtaacgcg taaacgatat
     5761 ggaatatttt gaaattccga aaagtacccg aatgtcatga acccgttgat gacgatattg
     5821 tccggctcta tattcgtgcc acccatcgga tgtatggggt tttcaccgat tcgttcaccg
     5881 aagctgtcat taaccttgta cacaatgcgt gcaaggcgcc gtgcttcgtc tatacgaaat
     5941 tgatttaccg acgtacgtgt gacggtaagt tcgtgccctg atgtgtccaa cgcggagaaa
     6001 cttcctacaa aacggcctgc atccaatatc gcatacgtac cgggcgccgt catcgggaac
     6061 tgaaaaagtg cggatgaatg gggatatttt tttatatcaa gctgaacaaa aaacgaatcg
     6121 tcgtccaatg aagtcagatc catgcggtaa gtcaactcag gtaattgcgc aaatgccgaa
     6181 acagctttgt gcgcaaatat aatccataag aaaaatgccc gccgcgtcat gaaatcctca
     6241 agccgtttgc aacaggtcgc tcggtttgaa gcaatacaac cccttccggt gttaccacgc
     6301 cgagcacaag acattccgaa aagaaattgg caatttgttt gggtgggaaa ttgacaacag
     6361 cgaggatttg ttttccgatg agggcttgtt tgtcatacat atccgtgatc tgtgcactcg
     6421 agcgtttgat gcctgccact ccaaaatcaa ttgtgagttg ataagcgggc ttccgtgctt
     6481 tgtgaaaatc atttacttcg atgatcgtac cggtgcgaat ttccaccttg tcaaaatcgt
     6541 cgtagctgat catagttttc cgctcagata ggctaatccc tgaataccca aatcgggttt
     6601 gatttcatga atagtgcgcg aaactttggc aaatttggcg atcatgcctc cgctggccaa
     6661 aacaggcata tccggttttc ctaattgctc acgcatacgc gttacaagcc cgtctatcat
     6721 acccgcataa ccaaagatca caccgctgcg caccgcatca tcagtatttt gaccgatcgg
     6781 cgaattcggt acatccaaat aaactgtttt aagtaatgcc gcttttgcaa tcaatgattc
     6841 gaccgaacct ttgatacccg gcgaaatagc accgccttga aaaacaccat cgctcacggc
     6901 aaaaaccgtc gtcgccgtgc ctgcggaaac aatgatgaca tcttttcctt tcagacgaac
     6961 cgccgcttcg gctaatatca agatatccgc accaacctga tgattgattg ccggatgaaa
     7021 tccataactc aaaggtgact taacatcaat aaaagatgat ttcactccac gggtttcttc
     7081 caataactga aacgctatgg tgaccgacgg cacgacactg gcaatcagca gctccggtat
     7141 cgtatccaat ttggcctcac ggcaaacatt ctgaagttga aaataatatt catcaacgga
     7201 gcgttccgaa tccgtacgaa accgccatac gtccacaaca cgctcgccgt ccagcacgcc
     7261 gattttgata tgcgtattcc cgacgtcaat tcccagtctt gtgctcatac ttgcactccc
     7321 aatttttttc cgaccgcttg aataaattca aatttgcctt gtgcgtacgt atttttatct
     7381 tcaggtacgt tttcagataa tttatttttt aaatccgcat acgcatcccg caaagcctta
     7441 tcacggcgca aaacatcacg aaacgtaata tgacgtcccc aaaacggcga cgttctgaca
     7501 gccgcatgaa tatgaaataa atgatcgcgt gctccgtaat ccggattcca atttatggat
     7561 ttctcctcga cattgttttt tggtctaata aaaaaacgtc tttctggaat atatttttcg
     7621 tactccggta tataccgata atccgcatcc gtcaatttag acaccatcgc ttggagttcg
     7681 atcatatcgt cagttccgat caaaatatcc acgacaggtt ttgccgctaa acccggtaca
     7741 gcggtacttc cgatatgttc gatactcaag gtcttatcag gccaaaattt cgttaaccgc
     7801 cgggattcgg attcaaatag gacagcccac tgcgcatcat acgcgataac atgaattttc
     7861 aatgatatag tacagataag gttataggaa agtgaatatc ccgtattgca ataaacataa
     7921 ccaatgcgta ttattttttc aaacaataac aggaatgatt tctgatgaaa tttttaatac
     7981 tcaatggccc caatcttaat ctgcttggcg agcgcgaacc gcacatctac gggactcaga
     8041 ctttagcaga tatagaggat aatctcaaaa ccgtttttcc ggaacatacg ctcgaattca
     8101 agcaatccaa catggaaggg gaattaattg atttattaca cgctgtgcgt aaaacgcaca
     8161 acggcgtggt ttttaatccg ggggcatatt cgcattattc tatcgcgtta caggacgcta
     8221 ttacatctat tcaaattccc gtcgtggagg tgcatttatc caatattcat gcccgtgaag
     8281 aatttcgcag ccgcagcgta gtggcccggg catgcatcgg ccaggttagc ggtttcggta
     8341 gctatgggta cgagatggcc attcgtgcac tgatacagca tcacacaaaa tagtcggtat
     8401 ttaccgcgat attatacaat ttcaggccgt ctctgttatt acaaattcag cttccacgat
     8461 cacttcggtt ttcatagaat tggcaatcag gcatttttta tgtgcatcat gcatgacgga
     8521 gcgcgctttt tctaaatccg cttcgttatc tacagcaatt ttggggcgca ctatgatttt
     8581 tgtaaaccga taaccgcctt caacaaattc taaaataccg ttggcccggc tttgataatc
     8641 ggcaatgtgt aatccggctt tttccgtaaa tgaaataaac gtcgtcatgg tacaaatttc
     8701 agccgccgcg acgaacaaat cttccggcgt ccagacatcg gtttcacctt taaactccgg
     8761 cgggctggca acacgaaaag ccggtttatc ttcggatgaa agcatcgctg cacgcgttgc
     8821 tatacgatgc aaaccggtat gataggtaaa tgttttatat ttgggtttgg gggctacagc
     8881 ttccataatg cgcctctata atattggaat agttttttat ttgtatataa ctatatagat
     8941 atatagagat caatcaaatg acttttatca taaaaaaacc cgccaaaaat atctggcggg
     9001 ttttatagaa tctttatgaa agataattat tttttcttct tcttatcttt ctttttgtct
     9061 tttttcttat ccgatttttt agacttctta tccgatttct
//
LOCUS       DAOQNQ010000113         9070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2821_length_9070_cov_5.856905, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000113 DAOQNQ010000000
VERSION     DAOQNQ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9070
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2821_length_9070_cov_5.856905"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PK754_08325"
     CDS             complement(<1..446)
                     /locus_tag="PK754_08325"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNB09548.1"
                     /translation="MGSGKTTVGAFVAQRMNFPFFDTDHIIEKKTGRTVSDLFADFGE
                     TYFRDAETVCLKETVQNYHQGIFALGGGALLRRENQVLVKNSGALIHLAVSHAEIIRR
                     IGRDGNRPLWDADRLDRLMEAREEGYRTADRSFDTDGFTAQSVADAV"
     gene            complement(476..1423)
                     /locus_tag="PK754_08330"
     CDS             complement(476..1423)
                     /locus_tag="PK754_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB09549.1"
                     /translation="MARSAYFKGVFFICLSAVCWSSAGLFIKLVNLPPVHIAMYRSLI
                     AGIFLLGYILYRRHARDIDFDFSVNRHGLITAIFYFFTVSLFVTANKMTTAANAVFMQ
                     YTSPVFVILITYFFLKEKIYRAEILTVVICLGGMVLFFLEEEKSTAFVGNMIAILSGV
                     AFAFLQIYVKRSETGTAEALTADQHHLRSIFAIVIGNLVTVMMGFVSAMFLMHAVTMP
                     DALAPIFGTQFTITTSDIIGLLILGIFQLGCGYLFFAAGAKYISSVEIAIYTILEPIL
                     NPIWTFLGTGEVPGFWAIIGANLIVAAMLVNTFFNKERA"
     gene            1615..1902
                     /gene="gatC"
                     /locus_tag="PK754_08335"
     CDS             1615..1902
                     /gene="gatC"
                     /locus_tag="PK754_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNB09550.1"
                     /translation="MPVTRTDVEKIASLAHLEFSDADLEKFTGQLNQILTYVEQLNTV
                     DTTDVEPTYHPITYPDVFREDQVAESLPVDKVLANAPDKTWQYIAVPKVVG"
     gene            1978..3615
                     /locus_tag="PK754_08340"
     CDS             1978..3615
                     /locus_tag="PK754_08340"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNB09551.1"
                     /translation="MARKKAHPKFIFVTGGVISSLGKGIAASSLGMLLKEHGYNVTIQ
                     KFDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDDNMSTRNSHTTGKVYK
                     TVIDKERRGDYLGATVQVIPHITDEIRGRIRTLTNEGYDIIITEIGGTVGDIESLPFL
                     ESIRQFCLELDPQDTVKIHVTLVPFIKAADELKTKPTQHSVMKLREIGIQPDILLCRS
                     ERTLSKDMRAKIGLFCNVPSFAVIEASDVRSIYEVPLIMHNNGMDQLVLKKLDLPSKK
                     LNLAHWKNLVHKIYEPKNKVRIALCGKYTGLPDAYKSILESFIHAGVENNANVDVAFI
                     DSSKIDEKTDLRHTFAGIDGILVPGGFGERGIEGMIRAIRYAREHKLPFFGICLGMQC
                     MSIEFARHVCGFSDANSSEFSKVTKHPVIDLMESQKDIKEMGATMRLGAYPCVVKDRT
                     KARSAYGSDLISERHRHRFEFNNRFIPDFEKKGMVFSGLSPDHKLVEIIELKEHPWFL
                     GVQFHPELKSRAIKAHPLFREFIKAALGHQRTHGKKK"
     gene            3625..5235
                     /gene="nadB"
                     /locus_tag="PK754_08345"
     CDS             3625..5235
                     /gene="nadB"
                     /locus_tag="PK754_08345"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNB09552.1"
                     /translation="MPSSYTSDFLVIGTGIAGYSCALKLAEMGTVAVITKKNHRESNT
                     NYAQGGIASVIDPDDSFDAHVRDTLNAGAGLCHRDAVELIVRQGPSAIDELIRIGVEF
                     TRHDDRLDLGKEGGHSTHRIVHAKDLTGREIERALIEAVKRHPNIQVFEDHIAVEVIT
                     EHHLLQKLTAYANIHCYGAYALDIPKNIVKKFIAKVTILASGGCGQVYKHTTNPKIAT
                     GDGIAMAYRAGARVGNLEFMQFHPTSLYHPDGESFLISEAVRGFGAYLINHAGERFME
                     RYSPMKELAPRDIVARAIDAEMKKRGEPCVYLDLRHIEAHKTLEHFPNISRRCAELNI
                     DMTREPIPVVPAAHYMCGGVVTDLYGRTNIHHLYACGEVAMTGVHGANRLASNSLLEA
                     VVFAHRVADHIRTASDDIEHKIPEFPDWDDSGTFDTEEWVLIAHNRAEIQEIMWNYVG
                     IVRSNLRLERAERRIGMIVEETENFYKKTKVTEELLELRNLAKLARLIIRCAKSRHES
                     RGLHYTTDYPEKDDHRWLKDTITSTFDA"
     gene            complement(5359..6117)
                     /locus_tag="PK754_08350"
     CDS             complement(5359..6117)
                     /locus_tag="PK754_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09553.1"
                     /translation="MKRIVFFLISTIAIMMGCDPKQNSDLTIPDVTNVLIMSPVMDFT
                     DVANTFPTYTKGIQYTTWNCNDSVPSPAYLRTFDAVLLFSNNGSSRADAVGDSLYAYV
                     MDGGALILGVFYNYHRSDTTITQYWGDLETIDPFIGRGNYYGNDTLVLDGNHDLLNGL
                     DTLATYYHANNLRIRSGAKVAAFYRSGNPAIAYNKPNGRIVAVNAFPAEFKYNAVTRV
                     EFYRLWENALLYAAWGTTDDRPNDHMPNEASIKE"
     gene            complement(6206..7429)
                     /locus_tag="PK754_08355"
     CDS             complement(6206..7429)
                     /locus_tag="PK754_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09554.1"
                     /translation="MKRILFTFAALIVLGGSAFAGIHSVPASAEDIQATYPNLVMPED
                     MISGEVNCMLVAGDVLYVGGRFEKMDRRVVNNVVKWDGTKWSGLNKGVDGVVYSMAMI
                     GTDLYVAGDFSYVDVNIEAGKIARWDGTKWNALSPQTVDRQIYALAVLGNDLIIGGNF
                     QKVGGTIETKGVAKWNGKAWSDVGMAKFDRTVLTLTVMNNEIYAGGFFTLVGDEPATS
                     VAKWDGKAWTEVGNKGINGTVTFMTNDGKNVYAGGKFTNAGDGTVLNRVAMFDGQKWQ
                     PLGEGVDGDVKSIHISGTDVYVSGTYREAKGGTAAAPAKEETAKGKDKKKGSKEDKGK
                     AKAKGPVQTQGIAKWDGNGWTGYPVIGKYAVGTFASAMFKGTLYVGGNFMANEGAVST
                     GTAKLENGAWGPVSK"
     gene            complement(7843..8442)
                     /locus_tag="PK754_08360"
     CDS             complement(7843..8442)
                     /locus_tag="PK754_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB09555.1"
                     /translation="MKKYILCFLTLWVLSCGGDSDSSVSYSETVERGATALNEGSYAE
                     AIKHFSDALAMNADRPEAFAGLGWAYLFSDSVEASEAAFSDGSMLANPGADLQAGYAF
                     LLGVKKNHASSNARIVSVLAAEPQWSLSYGLNIDYKDLYLLKAENHFLLGEYASSLTA
                     VKQIQSAFNVNTNTAEGIAQLASKIESLKLLTKTLARQM"
     gene            complement(8463..>9070)
                     /locus_tag="PK754_08365"
     CDS             complement(8463..>9070)
                     /locus_tag="PK754_08365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB09556.1"
                     /translation="DERSHLTVPAGQSSGSDWILTTIEETAGEIVYNYQAKSLPPYTR
                     FEYVADMSQMPAGMRPVISRWDGVQWVVRPSIYNERTHIVSTEISEAGQFKILFVNDD
                     TPSAIIPEDLQLEQNYPNPFNPSTHIRFAVPQASNVRLTVFNLLGQKVRTLIEDYRSS
                     GSYGVVWDGRNDAGLGVASGIYVYRLEAAGRIVSRKMMLMK"
BASE COUNT         2523 a         2273 c         1988 g         2286 t
ORIGIN      
        1 accgcatcgg ctacggattg ggcagtgaac ccgtccgtat caaagctccg atcggctgtc
       61 cggtatcctt cctcccgtgc ctccataagc ctatccaagc gatccgcgtc ccataaagga
      121 cggttaccat cgcgtccgat acgacggatg atttccgcat gcgaaaccgc taaatgaatc
      181 aacgcaccgg agttcttgac caaaacttga ttttctcggc gtagtaacgc tccacccccc
      241 aacgcaaaaa taccctgatg gtaattttgc accgtttcct tgaggcagac ggtttcagcg
      301 tcacgaaagt acgtttcgcc gaaatcggca aacaaatcgg agacggtgcg gcccgttttt
      361 ttttcgatga tatgatctgt atcaaaaaac ggaaaattca tccgctgcgc tacgaatgcg
      421 ccgaccgtcg ttttgccgga acccataaat ccgcatataa atatccgacg ggtattcatg
      481 cgcgttcttt gttaaaaaaa gtattaacca gcatagccgc aacgatcaga ttggcgccga
      541 tgatggccca aaagccgggc acctcaccgg ttcccaaaaa agtccagatc ggattgagaa
      601 tgggttccaa tatcgtgtat atggcgattt cgacactgga tatatatttg gcgccggcag
      661 caaaaaacag ataaccgcac cccagctgaa aaatgcccaa gatcaaaagg cctatgatat
      721 cggatgtcgt aatggtaaat tgcgtaccga aaatcggtgc caacgcatcc ggcatcgtaa
      781 cggcatgcat caaaaacatg gccgatacaa aacccatcat gacggtcact agattaccga
      841 tgacaattgc aaatatcgaa cgtaaatgat gttgatcggc tgtgagggct tcggccgtcc
      901 cggtttcgga acgtttgacg taaatctgca aaaacgcaaa cgcaacaccc gaaagaatag
      961 ctatcatatt gccgacaaaa gcggttgatt tttcctcctc caaaaagaaa agcaccatcc
     1021 cgccgaggca aataaccacc gtaagaattt ccgcccggta tattttttct tttaaaaaaa
     1081 agtatgtgat cagtatgaca aaaaccggcg aggtatattg catgaacacg gcattggccg
     1141 ccgtggtcat tttattagcc gtgacaaaca gtgaaacagt aaaaaaataa aaaatggccg
     1201 tgatcaatcc gtgccggttg accgaaaaat caaaatcaat atcccgggca tgacggcgat
     1261 acaagatgta acctaacaaa aaaatacccg caatcagcga acgatacatc gcgatatgga
     1321 ccggcggcag attgactaat ttgataaata aacccgcgct gctccaacac acggcggaca
     1381 aacaaataaa aaaaacgcct ttgaaatacg cagaacgcgc catagataat tccgattttg
     1441 attttgggga atataaagca ttccttgtga ttcacaacgg taaattgcgc taccggtttt
     1501 tcgctaaata tctttgtggt aatctgtttt tagtcctttg acgtaaggaa aataaatttg
     1561 acaaaaacgg ggctttcaca tatattgccc gcgttaaaaa aaagagcgca acctatgccg
     1621 gttacacgta ccgatgtcga aaaaatagct tcccttgcgc atttggaatt ttccgatgcg
     1681 gacttggaaa aattcaccgg ccagctcaat cagatactga cgtatgtgga gcagctcaat
     1741 accgtagata cgacggatgt ggagcccacc taccatccga tcacctaccc ggatgtattt
     1801 cgtgaagatc aggtagccga gagcctcccg gtggataagg ttctcgctaa cgcaccggat
     1861 aaaacctggc agtatatcgc ggtgccgaaa gttgtcggct aaaaaccaaa agagacacgg
     1921 aagcgtgtct ctttttttat aattaccttt catcattaag tttcccttta aaaaactatg
     1981 gcgcgtaaaa aagcacatcc gaaatttatt tttgtaaccg gcggcgtgat ttcgtccctc
     2041 ggcaaaggca tcgcggcatc cagcctcggc atgctcctca aagagcatgg ttacaatgtc
     2101 accattcaaa aatttgatcc ttacatcaat gtggatcccg gcacgatgag cccatatcag
     2161 catggcgagg tcttcgtcac ggatgacggc gcggaaaccg atcttgatct cgggcattac
     2221 gagcgcttta ttgacgataa catgagcacc cgcaattcgc acaccacggg taaggtttat
     2281 aaaaccgtga ttgacaaaga acggcgcggt gactatctcg gtgccaccgt acaggttatt
     2341 ccccatatca cggacgaaat tcgcggccgt attcgcacac tgaccaatga aggttacgat
     2401 atcatcatta cggaaatcgg cggcacggtc ggcgatatcg aaagtctgcc atttttggaa
     2461 tctatccgcc agttctgttt ggagttggac ccgcaggata ccgtcaaaat ccatgtgacg
     2521 ctcgtgccct ttatcaaagc ggcggacgaa ctgaaaacca agccgactca acacagcgtg
     2581 atgaagctac gtgaaatcgg tattcaaccg gatattttgc tatgccgctc ggaacgcacg
     2641 ctgtcaaaag acatgcgcgc caagatcggt ttattttgca atgtaccttc atttgccgtg
     2701 atcgaagcgt ccgatgtgcg ctccatatac gaagtgccgc tcatcatgca caacaatggc
     2761 atggatcagc tggtgttaaa aaaacttgat ctgccgagca aaaaactcaa tctggcacat
     2821 tggaaaaatc tggttcacaa aatttatgaa ccgaaaaata aagtgcgtat cgcgttgtgc
     2881 ggcaaatata ccggcctacc cgatgcctat aaaagtattt tagaatcctt tatccatgcc
     2941 ggtgtggaaa ataatgcgaa tgtcgatgtc gctttcatag actccagcaa gattgacgaa
     3001 aaaaccgatt tacggcatac gtttgccggc atagacggta tactcgttcc gggaggtttc
     3061 ggtgaacgcg ggatcgaagg catgatccga gccattcgtt atgcacggga acataaattg
     3121 ccgtttttcg ggatatgcct cggcatgcag tgcatgagca tcgaatttgc ccgccatgtc
     3181 tgcggttttt cggatgccaa tagcagcgag ttttccaaag tcaccaaaca tccggtgatt
     3241 gatttgatgg aatctcaaaa agatattaaa gaaatgggcg ccacgatgcg ccttggtgcg
     3301 tatccgtgcg tcgtcaaaga tcgcaccaaa gcgcgctccg cctacggatc ggatctcatc
     3361 agtgaacgcc accgccaccg gtttgaattt aacaatcgat ttatccctga ttttgaaaag
     3421 aaaggaatgg tattttccgg cctttctccc gatcacaagt tggtcgaaat catcgaactc
     3481 aaagagcatc cttggtttct cggcgtacaa tttcatcccg aactcaaatc acgcgcgatc
     3541 aaagcacatc cgctgttccg cgaatttatc aaagcggctc tggggcatca gcgtacacac
     3601 ggtaaaaaga aataattcgc accgatgccg tcgtcataca catccgattt ccttgttatc
     3661 ggcacaggca ttgccggata ttcttgtgca ttgaaattgg ctgaaatggg aacggtcgcc
     3721 gtcatcacca aaaaaaatca ccgcgaaagc aataccaatt acgcacaggg cggcattgcc
     3781 agcgtcattg atcccgacga tagttttgac gcacatgtcc gggataccct caatgccggc
     3841 gccggtttgt gccatcggga tgcggtcgaa ctgatcgttc gccagggccc ctctgcgatt
     3901 gacgaattga tacgtatcgg tgtcgaattt acacgccacg acgaccgcct tgatctcggc
     3961 aaagaaggcg gacacagcac gcatcgtatc gtgcatgcta aagatctgac cggccgcgag
     4021 atcgaacggg ctctgatcga agccgtgaaa cgccacccca atatccaagt attcgaagat
     4081 catatcgccg tcgaagtcat caccgaacat caccttcttc aaaaactcac ggcgtatgcc
     4141 aatattcact gctacggcgc atacgcgctc gatattccca aaaatattgt aaaaaaattt
     4201 atcgccaagg taaccatcct tgcatccggc ggttgtggtc aggtgtacaa acacacaacg
     4261 aatccgaaaa tcgccaccgg cgacggcatt gcgatggcgt atcgggcggg cgcccgcgtt
     4321 ggtaatttgg aattcatgca gtttcatccg acttcactgt atcatcccga cggagaatcc
     4381 tttctcatca gtgaagcggt acgcggtttc ggcgcctatc ttatcaatca tgccggcgaa
     4441 cgttttatgg agcgttattc acccatgaaa gaactggcgc cgcgtgatat agtggcccgt
     4501 gcgattgacg ccgaaatgaa aaaacgcggc gagccgtgcg tatatctgga tttgcggcat
     4561 atcgaagcac ataaaacgct ggaacatttt cccaacattt cccgccgctg tgccgaactt
     4621 aatattgata tgacacgcga acccatcccg gtggtaccgg ctgcacacta tatgtgcggc
     4681 ggcgtggtga cggatttgta cggccgtacc aatatccatc atctctatgc ttgtggcgaa
     4741 gtggcgatga ccggcgtaca cggtgccaat cgtcttgcca gcaactccct gctcgaagcg
     4801 gtcgttttcg cacatcgggt agccgatcat attcgcaccg catcggacga tatcgaacac
     4861 aagatacctg aattcccgga ttgggatgac agtggtactt ttgacacgga agaatgggta
     4921 ctgatcgcac acaatcgcgc cgaaatacag gaaatcatgt ggaactatgt aggcatcgtg
     4981 cgctccaatc tccgtctgga acgggccgag cgccgtatcg gcatgatcgt cgaagaaacg
     5041 gagaattttt acaaaaaaac caaagttacg gaagagttgc tggaactgcg taatttagcc
     5101 aaactggcgc gcctgatcat tcgctgcgcc aaatcccgtc acgaaagccg cggcttgcac
     5161 tatacgaccg actatcccga aaaagacgat caccgctggc tgaaagatac tatcaccagc
     5221 acatttgacg cataaaaaat tataactata aaaacaaaaa acgccgcggg atattctcct
     5281 gcggcgtttt tttgttttag atataaaata ctattgagcc tcaacgactg cagtttgatt
     5341 tacattaatc atattctttc attctttgat tgaggcttca ttcggcatgt gatcattcgg
     5401 gcgatcatcc gtagttcccc atgcggcata taataacgca ttttcccata aacggtaaaa
     5461 ttccacccgc gttaccgcat tgtatttaaa ttcagccgga aaggcattca ctgcaacaat
     5521 tcttccatta ggcttattat acgctatagc cggattgccc gagcgataga aagctgccac
     5581 tttcgcacca ctgcgtatcc taaggttatt ggcatgataa taggttgcca atgtatcaag
     5641 tccgtttaat aaatcatgat taccatccaa taccagcgta tcatttccat aataatttcc
     5701 tctgccgata aacggatcta tagtttcgag atcaccccaa tactgcgtaa tcgtagtatc
     5761 gctccgatgg taattataaa atacacccaa tatcaatgcg ccgccatcca tcacatacgc
     5821 ataaagacta tcaccgacag catcggcgcg gctgctgcca ttattactaa ataacagcac
     5881 ggcatcaaat gttctcagat aggccggtga cggcacggaa tcattacaat tccaagttgt
     5941 gtattgaata cctttcgtat aggtcggaaa ggtattagct acatctgtaa aatccattac
     6001 aggcgacatg atcaatacat tagtcacatc tggtattgtc agatcggaat tttgtttagg
     6061 gtcgcaaccc atcataattg ctatagtaga aattagaaaa aaaacaatcc gtttcatgtg
     6121 tttatatctc cgtcctgatg ttttattaaa tgcgaaggag ctatagaaac atagctcctt
     6181 caagttattc atacgataac ctcaactatt tagaaaccgg cccccaagcg ccattttcaa
     6241 gtttggcggt acccgtgctg acggcgcctt cattggccat aaagtttccg ccgacataca
     6301 atgttccttt aaacatagcg gaagcaaatg taccaacagc atatttaccg attacgggat
     6361 atccggtcca accattgccg tcccatttag ctatgccctg agtttgtacc gggcctttgg
     6421 ctttggcttt acctttatct tctttgctac cttttttctt atctttgcct ttagcggttt
     6481 cttcttttgc cggagcagcg gcggtaccac ctttggcttc tcgataagta cccgacacat
     6541 acacatccgt tccggagata tgaattgatt ttacatcacc atccacacct tcgccgagag
     6601 gttgccattt ttgtccgtca aacatagcta cgcggttaag caccgtaccg tcgccggcgt
     6661 tggtaaattt tccaccggcg tacacatttt ttccgtcatt ggtcatgaat gtcaccgtgc
     6721 cgttgatacc tttattgccg acttccgtcc atgctttacc atcccatttc gccacggaag
     6781 tagccggttc gtcgcctaca agcgtaaaaa aaccgccggc atagatttca ttattcatca
     6841 ccgtcagagt caaaaccgtt ctgtcaaatt tggccatacc cacatcgctc caagctttgc
     6901 cgttccattt ggcaacacct ttggtttcga tcgtaccgcc gactttttgg aaatttccgc
     6961 cgatgatgag atcattgccc aacaccgcca aggcataaat ctggcgatcg acggtctgag
     7021 gtgacagcgc attccattta gtaccatccc atcgtgcaat ttttccggct tcgatattga
     7081 catcgacata actaaaatca ccggccacat acaaatcggt accgatcatg gccatcgaat
     7141 acactacacc gtccacgcct ttattaagac cggaccattt ggtgccatcc catttgacga
     7201 cgttgttgac tacacggcga tccatttttt caaatcgtcc gccgacgtac aatacatcgc
     7261 ctgctacaag catacaattg acttcaccgc taatcatatc ctccggcatg accagattag
     7321 gatacgtagc ttgaatatcc tctgcggatg cgggaacgct atggattcct gcaaatgccg
     7381 atccaccaag gacgatcagc gcggcaaacg tgaacagaat tcgtttcatg tgttttttcc
     7441 tttcttgtta tatatgataa tgagaaccga acctacgtga ataaacgcaa aaaactccgc
     7501 gcaagccgac cggtgaacgt cttcgccggc caacttatgg gaggtacttg cgcggttatg
     7561 tgataaaact tttgtccgct gataaagaaa gatgatataa tgcagaagcc ggccgaatga
     7621 agcggttgtg caataatgca gatattgtgt ttggcatact tgttctccca taagtttgta
     7681 aacacgccat taacataagg tgaaaatcgg gattgggggt cttttgattg acgaaaaggg
     7741 ggattgggtt gacgaaaagg caggttttag taacaaaaaa cgcctgttct tttcagatca
     7801 ggcgttttat aataattaaa gctttagttt tttttgtcaa aattacattt gacgcgccag
     7861 agttttagtt aacagtttca aagattcaat tttagaggct agttgtgcga tgccctctgc
     7921 cgtgtttgta ttaacattaa acgctgattg aatctgtttg accgcagtaa gactcgacgc
     7981 gtattcaccg agaagaaaat gattttccgc tttaagcagg tacaaatcct tgtaatcaat
     8041 attcaagccg tagctcaaag accattgcgg ttcggcggcc aatacgctaa cgatacgcgc
     8101 gttggaagat gcatgatttt ttttaacgcc caataaaaac gcatatccgg cctgcaaatc
     8161 cgctccggga ttggcaagca tgctgccatc cgagaatgcc gcttcggagg cctccacgct
     8221 atccgaaaac aggtatgccc aacccaaacc agcaaaggct tcaggccgat cagcgttcat
     8281 cgctaaagcg tctgaaaaat gcttgatcgc ttccgcataa gaaccttcgt ttaaggccgt
     8341 tgcaccgcgt tcaaccgttt cactatacga aacagaactg tcggaatctc cgccgcagga
     8401 cagcacccat agcgtaagaa agcataaaat atattttttc ataacgtctc cggtaatttg
     8461 ggttatttca tcaacatcat ttttcgagaa acgattcggc cggctgcttc cagacgatat
     8521 acatagattc cggaggcaac gcccaatccg gcatcattac gcccgtccca gacaaccccg
     8581 tacgaaccgg atgaacggta gtcttcgatg agcgttctca ctttttgacc cagcaaatta
     8641 aatacagtaa gccggacgtt cgacgcctgc ggaacagcaa atcggatatg cgtagacggg
     8701 ttaaatggat tcggataatt ttgttcaagc tgcaaatctt ccggtatgat agccgatggg
     8761 gtatcgtcgt ttacaaataa aattttgaat tgacctgcct cggaaatttc tgttgaaacg
     8821 atatgtgtgc gttcattata gatcgaaggc cgaactaccc attgaacacc gtcccaacgt
     8881 gaaatcaccg gacgcattcc cgccggcatt tggctcatat cagcaacata ttcaaatcgg
     8941 gtatacggcg gcaatgattt tgcctgatag ttatatacta tttcgccggc cgtttcttcg
     9001 atcgtagtca aaatccagtc cgaaccggat gactgccccg cgggtaccgt caaatgtgaa
     9061 cgttcgtccg
//
LOCUS       DAOQNQ010000114         9017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2845_length_9017_cov_5.410623, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000114 DAOQNQ010000000
VERSION     DAOQNQ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9017
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2845_length_9017_cov_5.410623"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /gene="alaS"
                     /locus_tag="PK754_08370"
     CDS             complement(<1..1212)
                     /gene="alaS"
                     /locus_tag="PK754_08370"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNB09557.1"
                     /translation="MAHVLTAKEIRSQFIRFFEERGHRFVPSSPVVPNDDPTLLFTNA
                     GMNQFKPIFLGTEKRDYTRAANSQKCIRVSGKHNDLEEVGHDTYHHTFFEMLGNWSFG
                     DYYKAEAIQWAWELFTKQWGLPKDRLYATVYKTDDEAVETWKKVTDINPDHILRFGEK
                     DNFWEMGDTGPCGPCSEIHIDLTPDGNGGKLVNAGSPEVIELWNLVFIQFNRNADRSL
                     TELPAKHVDTGAGFERVARVLQGKTSNYDIDLFTDIISGIEKVAGKNYAESAIQAPFR
                     VIADHIRMLTFSITDGAIPGNDGRGYVLRRILRRAALYGRKLELHEPFIYKLVTNVVG
                     AMGDAFPEIRARQEFVERVIKTEEENFNKTLDRGIEVFEDMACKLESKGIKQIPGADV
                     FKLYDTFGFPAD"
     gene            complement(1307..2038)
                     /locus_tag="PK754_08375"
     CDS             complement(1307..2038)
                     /locus_tag="PK754_08375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB09558.1"
                     /translation="MSEIVKRHYDQLLADVYTWLYGGFDVQYTENLKFFKEQGIIPSG
                     KSIALDLGAGSGFQTLALNDLGFDVSSVDISDKLCSELGARAKGRKIEIYNSDILSFL
                     EVTTKEFEVIVCMGDTITHFDSKEDINKSIRLCSEKTVPGGSFILSFRDYSVELSGVS
                     RFIPVRSDAEKIFTCFVEYAPEKVYVHDILNTRISDKWEHKISVYPKLRLSVEWIKGV
                     LESNGLRITWSETSKGFVRLIARKG"
     gene            complement(2087..2437)
                     /locus_tag="PK754_08380"
     CDS             complement(2087..2437)
                     /locus_tag="PK754_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNB09559.1"
                     /translation="MTEPIKKIYYTISEVCEKTDLKQYVLRYWETEFDALKPLKNRSG
                     NRVYTEDDIDNILKIKKLLYEDKFTIEGAKKRMLGDTDQLSVAQVPQNNNREKAHHLI
                     EDIRLDIETLLKQL"
     gene            complement(2509..3624)
                     /gene="ald"
                     /locus_tag="PK754_08385"
     CDS             complement(2509..3624)
                     /gene="ald"
                     /locus_tag="PK754_08385"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNB09560.1"
                     /translation="MIIGVPKEIKTKENRVGLLPVGVAELKKHGHTVIVENNAGVGSG
                     FDNDAYVKAGAEIVPTPKDVYGRADMIVKVKEPIKPEYELIRENQVVFTYFHFAADRA
                     LTEAMIKSKCIAIAYETVQRSDRKLPLLDPMSEVAGRMAVHAGAKYLEKTFGGRGLLL
                     SGVPGTEPATVVVIGGGVVGTNAAKIAAGFGARVIMMDIDLYRLRYLDDIMPKNVHTM
                     MSNSYNIAEVIKHADLVIGAVLIPGAKAPHLITRDMLKTMKPGAVIVDVAVDQGGCVE
                     TTKPTTHEEPIYNIDGIVHYCVANMPGAVPFTSTVALTNATLPYAVQLANKGWEKAVK
                     ENHELLLGCNILNGKVVYKGVADAFDLPYVPVEQVVK"
     gene            complement(3653..4918)
                     /locus_tag="PK754_08390"
     CDS             complement(3653..4918)
                     /locus_tag="PK754_08390"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNB09561.1"
                     /translation="MKTLYDENNYTKVILDNGLTLVSQHMPHLRSVSIGVWITAGSRF
                     ESADTMGIFHFIEHSVFKGTKHRSALQIAKSLENVGGSINAFTSKEHTCFYANILSED
                     LTLAVDVLADLVIHARFDAKEINREKQVILEEIRDTEDTPEEFVQDYFYGQVYRNHPM
                     GYNILGTPQSVKVLDRKRIVDMYRKYYVPSNMVVSVAGNFDETTLQSVIAKKFVFPKN
                     LRNTSYWPAGFEKYTIRNTPSKTEVVEKKINQAHVCMGVTIHESYTDDHRFEYLVLNT
                     VLGGGMSSRLFQKIREKMGLAYSIYSFVDFLNDGGIFGVYWATDKNQAQKSVDTVKTE
                     LRKLADKPITVGELRMAKAQIKANLLFGLESTSTRMIRLAKNEIYLGKKINIGEITKL
                     IDRLTIRDIQKTGNTLAKLVDKMQISIVQ"
     gene            complement(4954..7143)
                     /gene="pnp"
                     /locus_tag="PK754_08395"
     CDS             complement(4954..7143)
                     /gene="pnp"
                     /locus_tag="PK754_08395"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNB09562.1"
                     /translation="MFTIKSRSMELGGRTLTIEHGRVARQADGAVWITYGDTTILATV
                     VCAKEMKEGIDFFPLMVDYRESSYSAGKIPGGFFRREGRPSEKEILSSRLIDRPLRPL
                     FPEGFFYDTIINVNVISSDQEGSADVIGGIGASAALMVSDVPFDNPIASVRVGRIDGQ
                     MIINPTFKQLETSDMDIVVSGTKDSIAMVEGESREISEEDMLNAILFAHDAIKQVIAL
                     QEELAKDIAPVKRTFDAATLPQEFINDITAIAKPVVYEADRIADKSLRNEKLKELKKS
                     LCEQFAEKYPEMDAAIKKMVDDLQYADMRSMILDEKRRLDGRTTCDIRQIVCQTSVLP
                     RVHGSALFTRGQTQALVATTLGTKLDEQIIDGVDSVETYKRFMLHYNFPPFSVGEARG
                     SRGPGRREIGHGNLAERALKNMAPSDAEFPYTIRIVSDILESNGSSSMASVCGGSLSL
                     MDAGVPMKSPVAGIAMGLIKESEKYAILSDILGDEDHLGDMDFKVAGTTNGITACQMD
                     IKIRGISTEIMREALAQAKDGRMHILGIMNTALAQPRPDISRYAPKITTIKIKQDLIG
                     LVIGPGGKMIRHIIEASGATIDIEDDGTVKIGAVNSENAEIAINMIKALTEEPEIGKK
                     YSGPIKKITEFGAFVEILPGKEGLLHISEIDYKRVAKVEDYFKLGDVVEVLLQDMTQR
                     DGKTKYELSRKALMPKPEGHVEPEKDANRGQGHEHRRSSHHHKKDRH"
     gene            complement(7402..7671)
                     /gene="rpsO"
                     /locus_tag="PK754_08400"
     CDS             complement(7402..7671)
                     /gene="rpsO"
                     /locus_tag="PK754_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNB09563.1"
                     /translation="MAVTKEQKIEVIKKHGGTATNTGATEVQIALLTQHINELTKHLQ
                     EHKKDHSSRRSLLKLVGQRKRLLTYLTKKDIEKYRSLIAELGIRK"
     gene            complement(7788..8720)
                     /locus_tag="PK754_08405"
     CDS             complement(7788..8720)
                     /locus_tag="PK754_08405"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNB09564.1"
                     /translation="MQILQGFDAINPNRPSVFTMGTFDGVHRGHQAVIKKLVEDAQAL
                     KARSVILTFDPHPQAVVNPEKAKSIHILTTIHEKQDIFAKLGVDALLIVKFDRSLMEK
                     TGQEFVDQFLIQRAGMKKLLLGYDHAFGKNRSGTFESLQPMAVEMNFIIEKLPAFDFD
                     RIVVNSTMIRNMLKDGDVSTANDLLGRRYIVEGTVQYGAGRGRTIGFPTANIQPTHMQ
                     KLIPMPGVYACRVNVDGSSFPAVTNIGQRPTFDTSLQTVIEAHILDFDRDLYDRSVGL
                     EFIYRIRDERKFSSREELIWQIRQDIRHAREQGF"
     gene            complement(8739..>9017)
                     /locus_tag="PK754_08410"
     CDS             complement(8739..>9017)
                     /locus_tag="PK754_08410"
                     /inference="COORDINATES: protein motif:HMM:NF027523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNB09565.1"
                     /translation="GKTVDIKPRRITIHAYHVLSYKENILRVMVQCSKGTYIRALARD
                     LGNELGCGAFVKSLRRLSIGAYFVENSSDVLNMAERIKQALATSTDKS"
BASE COUNT         2406 a         2136 c         1816 g         2659 t
ORIGIN      
        1 atccgccgga aatccgaacg tatcataaag tttgaataca tccgcacccg gtatttgttt
       61 tatgccttta gattccaatt tgcacgccat gtcttcgaaa acttctatac cgcgatcaag
      121 tgttttatta aaattttctt cttcggtttt gattacgcgc tcgacaaact cctgccgtgc
      181 acggatttca ggaaaagcat cacccatcgc gccgacgaca ttggtaacaa gcttgtaaat
      241 aaacggttca tgcaattcga gtttacgacc atatagtgca gccctccgca gaatgcgtcg
      301 caaaacataa cctcggccat cgttgcccgg tatagcgccg tccgttatag aaaatgtcaa
      361 catccggatg tggtcggcaa tgacccggaa cggcgcctga atagcggatt cggcgtaatt
      421 ttttcccgct actttttcaa ttcctgaaat aatatcagta aacaaatcaa tatcgtagtt
      481 cgaagttttg ccctgtaaaa ctcgtgcaac gcgttcaaaa ccggcaccgg tatcaacgtg
      541 tttagccggc agttcagtca ggctgcgatc ggcatttcga ttaaactgta tgaacaccag
      601 attccaaagt tcgatcactt cgggtgaccc ggcgttgacc aatttgccgc cattcccatc
      661 cggtgtaaga tcaatatgga tttctgaaca cggtccgcac ggccccgtat cacccatttc
      721 ccaaaaatta tctttttctc caaatcgcaa aatatgatcc ggattgatat ccgtaacttt
      781 tttccaggtt tcgaccgctt catcgtccgt cttgtacacg gttgcataaa ggcgatcttt
      841 aggcaatccc cactgcttcg taaaaagttc ccatgcccac tgtatcgctt cggctttata
      901 ataatcccca aatgaccagt tacccaacat ttcaaaaaat gtatggtggt atgtatcgtg
      961 ccctacttct tcgagatcgt tgtgtttgcc gctaacgcgg atacattttt gcgaattggc
     1021 cgctcgtgta tagtctcgtt tttcggtacc caaaaaaatg ggcttaaact ggttcatccc
     1081 tgcattagta aaaagtaacg tcggatcgtc attaggaaca accggactgg atggtacaaa
     1141 acgatgcccg cgctcttcaa aaaaacggat aaattgacta cgaatttctt tcgctgtaag
     1201 aacatgtgcc atgaaatcta ttataactcc aaataattga ttttacagat ataaatatat
     1261 aaacgctgtg cggattatca aaacaatttc aaataaaatc ggcaatttag cccttgcgtg
     1321 cgattagtcg cacaaaacct ttagacgttt cggaccatgt tattcgcaga ccgttggact
     1381 ccaaaacccc ttttatccat tccaccgaga gcctcaattt aggataaaca ctgattttat
     1441 gctcccattt atcgctaatc ctggtattta aaatatcgtg cacataaact ttttcgggag
     1501 catactcaac aaaacacgta aatatctttt ctgcatctga tcgaaccgga ataaaccgtg
     1561 aaacacctga aagttccacg ctgtaatcgc gaaaagataa aataaaggaa cctccgggaa
     1621 ctgttttttc ggagcatagt ctgatagatt tattaatatc ttcttttgaa tcaaagtgag
     1681 taatcgtatc tcccatacaa acaattactt caaattcttt cgttgtgact tccaaaaagc
     1741 ttagaatatc cgaattataa atttcaattt ttcggccttt tgcacgtgct cccaattcgg
     1801 agcaaagttt gtcacttata tcaacactcg aaacatcaaa acctaaatca tttagagcta
     1861 atgtttgaaa cccgctacct gctcccaagt ccaaagctat acttttgccg gatgggataa
     1921 taccttgttc cttaaaaaat ttaagatttt cagtgtattg gacatcaaac ccgccataca
     1981 accaagtata gacatctgcc aatagctggt cgtaatgtct tttgacaatt tctgacatat
     2041 cttttttaaa actgtgcgaa gtaaggctta aggagtgttt tacggattac aattgcttta
     2101 agagcgtctc tatatccagt cggatatctt cgatcagatg atgcgctttt tccctattat
     2161 tgttttgagg aacctgtgca accgagagtt ggtctgtatc ccccaacatt ctttttttgg
     2221 cgccttcgat cgtaaactta tcctcataca gtagtttctt tattttgagg atattatcta
     2281 tatcgtcttc cgtataaact ctgttaccgg aacggttttt aagcggcttc agcgcatcaa
     2341 attccgtttc ccaataccga agtacatact gcttcagatc ggttttttca caaacttcac
     2401 tgatcgtata gtatattttt ttgatcggtt cggtcataga ctcactccgg actaggctta
     2461 aaaacaagag cggtaacctt ttcagattac cgctctttgt ataaacgatt atttgacgac
     2521 ttgttctacc ggaacataag gaaggtcaaa agcatcagct acacctttgt aaacgacttt
     2581 tccattgagg atattgcatc cgagtaaaag ttcgtgattt tctttgacgg ctttctccca
     2641 gcctttattg gccaattgta cggcatacgg cagtgttgcg ttggtcaaag cgaccgtcga
     2701 cgtaaacgga accgcaccgg gcatattggc aacacaatag tgtacgatac cgtctatgtt
     2761 gtagatcggt tcttcgtgtg tcgtaggttt agttgtttca acgcaaccgc cctgatccac
     2821 agccacatcc acgatcacgg caccgggttt catggttttt agcatatcgc gtgtgataag
     2881 gtgcggggct ttggctccgg gtatcaaaac agctccgatc acgagatcgg catgtttgat
     2941 cacttcggcg atattataac tattcgacat catggtatgt acatttttgg gcatgatatc
     3001 atccaaatag cgcaaacgat acaaatcaat atccatcata atgacacgcg caccgaaacc
     3061 ggcggctatt tttgcagcat tggtacctac aacaccccca ccgatcacaa cgacggttgc
     3121 gggttcagtg cccggaaccc cgctcagaag taatccgcgg cctccaaacg ttttttcaag
     3181 atacttagcg ccggcatgaa ccgccatacg tcctgctact tctgacatcg gatcaagcaa
     3241 aggcaactta cgatccgaac gttgaaccgt ttcataagcg atcgcaatac acttggattt
     3301 gatcatcgct tcggtcaaag cgcgatctgc cgcaaagtga aaatacgtaa atacaacctg
     3361 attctcgcga atcaattcgt attccggttt gatcggttct ttgaccttga cgatcatatc
     3421 agcacgaccg taaacatctt taggggtcgg tacgatttca gccccggctt tgacatatgc
     3481 atcattgtcg aaaccgctgc ctacaccggc attattttcg actatcacag tatgaccatg
     3541 ttttttgagc tcggctacgc ctaccggcaa aagaccgaca cggttttctt tggttttgat
     3601 ttctttcggt acacctataa tcatgacttc gtctcctaga atttggtcca gattattgaa
     3661 caattgaaat ttgcatttta tctacaagtt ttgcaagcgt attccctgtt ttttggatgt
     3721 ctcggattgt aagtcgatca atcagtttag tgatttcacc gatatttatt ttcttgccaa
     3781 gataaatttc atttttcgcc aaacgtatca tgcgcgtact ggtactttca agaccgaaaa
     3841 gaagattggc tttgatttgc gctttggcca tacgcaattc tccgactgta atcggtttat
     3901 ctgcaagctt acgtaattcg gtttttaccg tatccacact tttttgggcc tgatttttat
     3961 cggtggccca atacactcca aaaatgcccc cgtcatttaa aaaatcaacg aaactgtata
     4021 tggaatacgc caaacccatc ttttcacgaa ttttttgaaa taaacgcgaa ctcatcccgc
     4081 cgccaagcac ggtattgaga actagatatt caaaacgatg gtcatcggta taggactcgt
     4141 gaatcgtcac tcccatacac acatgtgctt gattgatctt cttttctact acttcagttt
     4201 ttgacggagt atttcgtatg gtatattttt cgaaacccgc cggccagtag gaagtattac
     4261 gcaaattttt tggaaataca aattttttgg caattaccga ttgcagcgtg gtctcatcaa
     4321 aattacctgc aacagacacc accatattgg atggtacata atatttacga tacatatcca
     4381 ctatgcgttt gcgatccaaa acttttacgc tttgcggtgt tccaaggata ttataaccca
     4441 tcggatgatt tcgatacacc tggccataaa aatagtcctg tacaaactct tcgggcgtat
     4501 cttccgtatc acgaatttcc tctagaatca cctgtttttc gcgatttatt tccttggcat
     4561 caaatcgtgc atgaatcact aagtcggcaa gcacatccac ggctaaggtc aaatcttcgc
     4621 taagaatatt cgcataaaaa cacgtatgct ctttgctggt aaaagcattg atgctaccgc
     4681 cgacattttc taaacttttg gcgatttgca acgcgctacg gtgtttcgtg cctttgaata
     4741 cgctatgttc gataaagtga aatatcccca tcgtatcggc tgattcaaac cggcttccgg
     4801 ctgtaatcca cactccgata gataccgaac gcagatgcgg catatgctga gaaaccaagg
     4861 tcagtccgtt atccagaatg accttggtat aattattctc gtcgtataaa gttttcattc
     4921 ccgtttccgg gactatgaaa ttacgtggca cgcttagtgt ctgtcctttt tgtgatgatg
     4981 actactgcgg cgatgttcgt gaccttgtcc gcgattggca tctttttcag gttccacatg
     5041 accttcgggt ttgggcataa gcgctttacg gctcaattca tatttggttt taccatcccg
     5101 ctgcgtcata tcttgcaaaa gaacctcgac cacatcacca agtttgaagt aatcttctac
     5161 tttagcaacg cgcttgtaat caatctccga tatatgcaga agcccttctt ttccgggcag
     5221 gatttcgaca aacgcgccga attctgtaat cttcttgatc gggccgctat atttttttcc
     5281 gatttccggt tcttcggtaa gcgctttgat catgttaatt gcaatttcag cattttcgga
     5341 attgactgcg ccgattttga cggtaccgtc atcttcgata tctatcgttg caccggatgc
     5401 ctctataatg tggcgtatca ttttgccgcc gggtccgatc accaaaccga taagatcttg
     5461 tttgatctta atggttgtga tcttcggcgc ataccgtgaa atatccgggc gaggttgagc
     5521 cagcgcggta ttcatgatac ccagaatgtg catacgtcca tctttagcct gtgccaaagc
     5581 ttcacgcatg atttccgttg agataccacg tattttgata tccatctgac atgccgtgat
     5641 cccattggtc gtaccggcta ctttaaaatc catgtctccc aaatgatctt cgtcaccaag
     5701 gatatcgctg agaatcgcat atttttccga ttctttgata agtcccatag cgataccggc
     5761 tacgggcgat ttcatcggaa cgcctgcatc cataagtgaa agcgaaccgc cgcagaccga
     5821 agccatagag cttgagccat tggactctaa aatatcgctc acgatacgaa tcgtgtaggg
     5881 gaactcggca tcggacggcg ccatattttt gagtgcgcgt tcggctaagt taccgtgtcc
     5941 tatttcacga cgaccgggac cgcgcgaacc gcgcgcttcg ccgactgaaa acggcggaaa
     6001 attgtaatgc agcatgaatc gtttgtacgt ttctaccgaa tccacaccgt caataatttg
     6061 ttcatctaat ttggtaccaa gtgtggtggc tacaagcgct tgggtttggc cgcgggtaaa
     6121 aagtgccgat ccgtgaacgc gaggaagtac gctggtttgg catacgatct ggcgaatatc
     6181 gcacgtcgta cgaccgtcta agcgccgctt ttcatccagg atcatcgaac gcatatcggc
     6241 atactgaaga tcatcaacca tttttttgat tgccgcatcc atctccggat atttttctgc
     6301 aaactgttcg caaagcgact tcttcagttc ttttagtttc tcattgcgca gcgatttatc
     6361 tgcgatgcga tcggcttcat acacgaccgg cttagcgatc gcggtaatgt cgttgataaa
     6421 ttcctgcggt aacgttgcgg cgtcgaatgt tctttttacg ggcgcaatat ctttggctaa
     6481 ttcttcctga agcgcaatga cttgtttgat agcatcatgc gcaaaaagaa tcgcattaag
     6541 catatcttct tcggaaatct cgcgggactc cccctccacc atggcgatgg agtctttggt
     6601 gccggaaacc acgatatcca tatcgctggt ttctaattgt ttgaatgtgg gattgatgat
     6661 catttgaccg tcaattcgcc ctacacgcac ggaggctatc ggattatcaa acggaacatc
     6721 actgaccata agtgcggccg aagcgccaat accgccgatc acatctgcgc tgccttcctg
     6781 atccgacgaa atcacattga cattgatgat cgtatcgtaa aaaaatcctt cgggaaataa
     6841 cggacgaagc ggacgatcaa taaggcgact tgagaggatt tctttttccg agggacggcc
     6901 ttcacgacgg aaaaaaccgc cgggtatttt accggcagaa tacgaactct cgcggtaatc
     6961 caccatcagc ggaaaaaaat caatgccttc tttcatttct ttggcacaca cgacggttgc
     7021 caaaatcgtg gtatcaccat aggtaatcca cacggcacca tcggcttgac gcgctacacg
     7081 accatgttcg atcgtgagcg tacgcccgcc cagttccatg gatcgggatt tgattgtaaa
     7141 cataccattt ctccttaatt atgaggtatt actataaaaa gaaacggtgt gatgcgtttt
     7201 gtttgccaat ccgcacgtcg cgtcagccta agcgaaaact gtgcgtcagg tttcgtttag
     7261 aatgacgttg atgcagataa cagacgaatc ccaccatttc aatgaacata taaacaaaaa
     7321 caggctgtcc caaaaacgca ccgtgttcaa cctaacaaaa agagaacaaa acggatagct
     7381 tttgggacag cctgatacaa attacttacg gataccgagt tctgcgatca atgaacgata
     7441 tttctcaata tctttcttgg tcaaatacgt gagtaaacgt ttgcgctgac caacgagttt
     7501 gagcaacgaa cggcgcgagc tgtgatcttt tttgtgctct tgcaggtgct ttgtgagctc
     7561 gttgatgtgt tgcgtgagca gcgcgatctg gacttccgtg gccccggtat ttgtcgcagt
     7621 tccgccgtgc tttttaatca cttcgatttt ttgttctttg gtaacggcca tgtgattctc
     7681 tttactcctt tatgtttttg aaattattgg gcttactttt aacccaaaag cgcgtgaatg
     7741 tatataatgc aaatatgtaa gtcaattgtt tttttaataa aaaaaaccta aaagccttgc
     7801 tcgcgtgcat gacgaatatc ttgacgaatt tgccagatca attcttcgcg ggatgaaaat
     7861 ttacgttcgt cccgaatacg ataaataaac tcaaggccga cggatcgatc atacaaatca
     7921 cgatcaaaat caaggatatg cgcttcgatc actgtttgca aacttgtgtc gaacgtaggt
     7981 ctctggccaa tattagtaac cgcaggaaat gaggaaccgt ccacattcac acggcatgcg
     8041 taaacgcccg gcatcgggat gagtttctgc atatgtgtgg gttggatgtt tgccgtagga
     8101 aacccgatcg tgcgacctcg cccggcgcca tactgtaccg tgccttcgac gatataacga
     8161 cgacctaaaa ggtcattggc cgtagataca tctccgtctt tcaacatatt gcgaatcatc
     8221 gtgctattaa caacgatacg atcaaaatca aacgccggca atttttcgat aataaaattc
     8281 atttcgacag ccatcggctg taacgactcg aatgtgccgc tacgattttt tccaaaagca
     8341 tggtcatatc ctaacagtaa ttttttcatg ccggctcgtt gaatcaaaaa ttggtcaaca
     8401 aattcttgcc ctgttttttc catcaaggaa cggtcaaatt ttacaataag aagcgcatcc
     8461 acccccagct tggcgaagat atcttgcttt tcgtgtatcg tagtaagaat atgtatggat
     8521 ttcgcttttt caggattaac tacggcttgg ggatgaggat caaacgtaag aataaccgaa
     8581 cgcgccttca acgcctgcgc atcttcaact aattttttta taactgcctg atgaccgcga
     8641 tgcacaccgt caaatgtgcc catagtaaat accgatggtc tattgggatt tatcgcgtca
     8701 aaaccttgta aaatttgcat aaaatttaga ataaaaaatt atgatttatc ggtggatgtc
     8761 gctaatgctt gttttatacg ctcagccata ttcagcacat ccgaactatt ttctacgaaa
     8821 tatgccccaa tggataagcg acgcagagat ttgacaaagg ctccgcaccc taattcgttt
     8881 cccaagtcgc gtgccaatgc acggatataa gtccctttgg aacactgtac catcacccgc
     8941 aatatgtttt ctttgtaaga taaaacgtgg tatgcatgta tagttatccg gcgcggtttg
     9001 atatctacag tttttcc
//
LOCUS       DAOQNQ010000115         9005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2851_length_9005_cov_6.676313, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000115 DAOQNQ010000000
VERSION     DAOQNQ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9005
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2851_length_9005_cov_6.676313"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="PK754_08415"
     CDS             complement(<1..837)
                     /locus_tag="PK754_08415"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09566.1"
                     /translation="MAALSSIYFFSIRDARITTNFDLFLLTSLAFSWYFLARMTGLYD
                     EFRSRNFSYELVGLIKNILFQIVIAIVVMFIAKNHTLPRLFIVLYAAHLLIILSIERF
                     LVRRVLNFLRRKGRNLRHLLIIGAGEVGTAFNELIRSNSHFGYAVTGFLDDQPQVSLN
                     GKLLGKVEDLESVLQKHPVDDVIVALPTYASEKIEWIIKICDHHTRRVRIIPDYFRFV
                     SDKFEVRMFGRFPLISVRKIKMDEAHWYIIKRCFDAFFALAFMVLVLSWLVPVFPLLL
                     VAY"
     gene            complement(975..1901)
                     /locus_tag="PK754_08420"
     CDS             complement(975..1901)
                     /locus_tag="PK754_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020154095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNB09567.1"
                     /translation="MKKNDKIFVAGHKGLAGSAILRKLIDQGYTNLVLRTHQELDLEN
                     QTDVRVFFEKERPEYVFLAAAKVGGIHANNVYRADFLIKNLKIQNNVIESAYRFGSKK
                     LLFLGSSCIYPKLAPQPLKEEYLLTGLLEPTNEPYAIAKIAGIKLCESYNRQYGTDFI
                     SAMPTNLYGPNDNFDLNNSHVLPALIRKFVEARDRNASEVTIWGTGTPLREFLHVDDL
                     ADALIFLMMHYSGNEFVNVGTGREISILDLAHCIKRLTAYSGKLEFDSSKPDGTPRKV
                     MDISKLNAMGWYPKISLDTGLKQTIDWYEKHV"
     gene            complement(1901..2911)
                     /gene="gmd"
                     /locus_tag="PK754_08425"
     CDS             complement(1901..2911)
                     /gene="gmd"
                     /locus_tag="PK754_08425"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNB09568.1"
                     /translation="MNKKALITGITGQDGSYLAELLLEKGYEVHGIIRRASTFNTDRI
                     DHLYKDPHVNGVRMFLHYGDIADSANLIKLIHQIEPDEIYHLAAQSHVRVSFDIPEYT
                     GDVTGVGTARILEAIRMTGCKARFYQASSSEMYGLVQEVPQKETTPFYPRSPYGAAKV
                     YAYWLTVNYRESYNMFATNGILFNHESPRRGETFVTRKITRAVAHIAAGLQNKLYLGN
                     LDAKRDWGYAKEYVEAMWLMLQQEKADDFVVATGETHTVREFLEVAFGYVGKNYQDFV
                     DIDPVYYRPAEVDLLIGDASKARQILHWQPKTTFRELVELMVEADISLLEQTHKINIR
                     KK"
     gene            complement(2967..6383)
                     /locus_tag="PK754_08430"
     CDS             complement(2967..6383)
                     /locus_tag="PK754_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HNB09569.1"
                     /translation="MTENYRGYIHTRGITSSVFLYICFAVVLLFPFSRVCPQEVVPLE
                     LFSKKIFDNYSYLNGSSDKKESLENYFLSGFNELIVPSTLVEWESRRNRIVDSLKQFY
                     KLDQWLSAQYPLNAIYHDTLDRPLYSIVLLTFQSYDGVRVPATLYLPKNGLEKKPAVV
                     YAEGYYFDAYGYNFANVDHWQRMSQHLALRGYVVLSINQLGLGQSALAGDTYQYQGYK
                     FNFAGDAFKVRSRLAPFIQGINYLFSREDVDTTFIGITGYSAAGAIAVEVGAIDQRIA
                     CTIVNDGGIYKTKLINESERIDPRSTTFSGSEASIDDFVLFSLFPPRPMIFIDSNQEL
                     YENYIPAALDRFKKLYEMYNTASGFHFLTNVGNHNYNNEKRLIAYSYFDTILGLPAGL
                     NEHDPSIELFDTLFHPSSFLISDFETKNTDEILSEMIYANSLKSVSDLEDYDISEAYS
                     KLGVDTSWSVISVSVKDSLSSGPYTVLYETIVLKEPLRDGSKLEYPVVTLKRNDKRPK
                     NKVFFVAENEIGREVWKTDSSLSRQLTNLLDSGFIVTGIDLLGYGELDIQFPSTNSLN
                     GSGYYKEQWTTEWVGYSGQLLMGHRISSLIAILKYLEDNRADISSVLWGYGFSPSLVV
                     QFVANKYPKTGTTILSNYLFDWNQLLNTTYGYYPDLYEANLLKTIDPSAIIAKSRVPL
                     LIQDIMNETRVKVTEGYRSRFESIRGSYFFGPELARSELLNTQLNFDYDISDTLRFWL
                     NNKPQIISNYFFNAAEDAYFHSNILVNDPDTLLYESELRYRFDNLPQWLILDSVRGFF
                     YGTPTANDIGSYVIKLQVTDVAGDYDSASITLNVVHTNHPPLWRSEFKTDAKEGLIYD
                     AYVYASDKDSALFGDTVKYEILGGPKWMEIDSLTGKMSGMPPNNTHGDLPLNLRAKDQ
                     FGGFKDTMIVIHIEPTNYPPPVPIAVFPAMGDTITIFRKPLVYRFIWRASIDPDNEDT
                     VNYLLHFTGNNLDTMIKVKSDTSLDFDSAVLNEMSEYSLKLYAVDDEFSSMSDSSVTF
                     FTTQAYANRNYVDVPTDYKLHQNYPNPFGHMTNICFQIPELSNVTITFYNTIGQFVDR
                     IVLEKVEPKYYEVKWFATAKSSAVYFVRFEASSFVSRKKFQKVMKILHVK"
     gene            complement(6401..7171)
                     /locus_tag="PK754_08435"
     CDS             complement(6401..7171)
                     /locus_tag="PK754_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012928843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNB09570.1"
                     /translation="MAKIVGIEFYDQGIHRAVNVILNECKTIQKSNRIVSATDAHGIV
                     RSHSEREFKELLESFYLRLPDGMPTVWVGRIKGHSNMGRCYGPDFFKLVMQTSSTTPV
                     RHFLCGGKQGIAEQLRRVCSDRFGNNHVVGCYSPPFRDMTDEELRDLANQINDTSADI
                     VWIGLGSPKQEYFARRLSGYIGTHFLVTIGAAFDFHTGNIKQAPLWVQKLGLEWFFRL
                     CVEPRRLWKRYLYVVPRFFILGMHDIFIYLLHRRFGRL"
     gene            complement(7171..8367)
                     /locus_tag="PK754_08440"
     CDS             complement(7171..8367)
                     /locus_tag="PK754_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB09571.1"
                     /translation="MKTDALIFHRTLPHYRAEILGKLAEVMSFRWIHCSDQIDGLSSS
                     SANSHLAEKTSSITVRYFRNKGWTGENIIFMDVVTPILKYKPRFVFIQFELSMVSIWL
                     LFLIRKLYGIRIVLWGHGYDRKEIFDPRRSSKDKLRLYMMRQADAVLFYNANRKKDIL
                     SVLPSEEIAGKMFVANNTLNTDVLFQQYLSLQRDDFDVIKRKIGFTAKYNLIYIGRIV
                     QDKEPLFLIEVMRKLLISGLDVHLHVIGRGSLQSNMQALIDQYEMKPFVKMYGEILDN
                     KELGPFLYASDAFVMPGFLGLSIVHAFCFRCPVISKKTMPLGPFHSTEIEYLENGETG
                     LLLDHDIDRFTEGLYKFLVEDKERERMKKKIDEKIASACRIQVMQAGFQEAIRFINMQ
                     YEMSGD"
     gene            complement(8357..>9005)
                     /locus_tag="PK754_08445"
     CDS             complement(8357..>9005)
                     /locus_tag="PK754_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09572.1"
                     /translation="SHQYITLPYFWVGYFAFTILITNARMAHILLALVLLAFFLMKMS
                     LKNILSGAVLLVFIVISLYFSGFLDQLLENFQDLVWRITFDVDEYSSYENFAAGRYSR
                     EAAVLYYLNEPLKFFGDGPSKYYDPVTQEYLVGNNGLIFTFYSEIGLLGLGAGYMVIY
                     SIAREFSDKRVAFIPILFSFCMIGLTITTNVLSDMSIIFSFVLFLSINKKILYEN"
BASE COUNT         2995 a         1904 c         1541 g         2565 t
ORIGIN      
        1 ataggcgacg agcagcagcg gaaaaacagg caccaaccaa gacaatacaa gtaccataaa
       61 ggccaacgca aaaaacgcat caaaacaacg tttgatgata taccaatgcg cttcatccat
      121 tttgattttc cgaacggaga tgagcgggaa tcttccgaac atgcgcactt caaatttatc
      181 agaaacaaat cggaaataat ccggaataat gcgcacacgg cgcgtatgat ggtcacaaat
      241 tttgatgatc cactctattt tttcagacgc atatgtcggc agagcaacga taacatcatc
      301 gacggggtgt ttttgtaata ccgattccag atcttcgact ttaccaagta attttccatt
      361 aagagatacc tgcggctgat cgtcaagaaa acccgtaacg gcataaccga aatgagaatt
      421 ggatcgaata agctcattga aagctgtacc gacttcaccg gctcctataa tcagcaggtg
      481 ccttagatta cgtcctttac ggcgcagaaa atttaaaacc ctccgaacca aaaaccgctc
      541 aatggatagg ataataagta aatgggctgc gtaaagaacg ataaatagtc gcggtaacgt
      601 atgatttttt gcaataaaca tcactacgat ggcaatgact atttgaaaca aaatattttt
      661 gatcaaacca acgagttcat aactgaagtt acgtgaacga aactcatcgt ataaaccggt
      721 catgcgtgca aggaagtacc aggaaaaggc taaagaagta agaagaaaga gatcaaaatt
      781 ggtcgtaatg cgcgcatcgc gaatcgaaaa aaaataaatc gagctcaacg ccgccacgag
      841 cactcctacc gtgtctgcgg taacccgaag aaaatataac atccgacgct gcgtatgcat
      901 aaaaagctat acacttatcc acattattcc tgtttggaag ttacattttt tttactacga
      961 atgaaatcaa attattatac atgcttttcg taccaatcaa tcgtctgttt caaccccgta
     1021 tctaacgaaa ttttagggta ccaacccatc gcattcagtt ttgaaatatc cataactttt
     1081 cgaggggtgc catccggttt cgatgaatca aattccaatt taccggaata agctgttagc
     1141 cgtttaatac aatgtgcaag atcaagaata gaaatttcac gccccgtccc gacattaaca
     1201 aactcatttc ctgagtaatg catcatcaaa aaaataagcg catcggcaag atcatcaacg
     1261 tgaaggaatt cccgaagagg tgtacccgtg ccccatatcg ttacctccga tgcattacgg
     1321 tcccgtgctt caacaaattt tcttatgagt gccggaagaa cgtgggagtt attcaaatca
     1381 aaattgtcat taggaccata aaggttcgta ggcatagccg aaataaagtc cgtaccatat
     1441 tgacggttat acgattcgca taattttatt cctgcaattt tcgcaatcgc atacggctca
     1501 ttggtaggct ctaaaagtcc cgtaagcaaa tattcttctt taagcggctg cggagccagt
     1561 ttaggataga tacaggagct tccaagaaaa agtaattttt tggagccgaa ccgatacgca
     1621 gactcaataa cattattttg aattttgaga ttttttatga gaaaatcagc tctataaaca
     1681 ttatttgcat gaataccgcc aacttttgcc gctgcaagaa aaacgtattc agggcgctcc
     1741 ttttcaaaaa aaactcggac atcagtttgg ttttccaaat caagctcttg atgggttctt
     1801 aaaaccagat tagtataacc ctgatcaatc agtttgcgaa gaatggccga accggccaaa
     1861 cctttatgtc cagcaacaaa aatcttatcg ttctttttca ttatttttta cgaatattga
     1921 ttttatgcgt ttgttctaac aggctaatgt ccgcttctac catcaactcc actaactctc
     1981 ggaatgtagt ttttggttgc cagtgtaata tttgtctagc tttcgatgca tcgccgatca
     2041 acaaatcaac ttctgccgga cggtaataga caggatcaat atcaacaaaa tcttgataat
     2101 tcttaccgac atacccaaac gcgacttcta aaaattcacg taccgtatgg gtctcaccgg
     2161 tggcaactac aaaatcatcc gctttttcct gctgaagcat caaccacatc gcttcaacgt
     2221 attctttggc atatccccaa tcccgtttgg catcaagatt acccaaataa agtttattct
     2281 gtaaccccgc tgcaatatgc gccaccgcgc gcgttatttt tcgcgtaaca aaagtttctc
     2341 cgcgtcgcgg cgattcgtga ttaaataaaa ttccattggt agcgaacata ttatacgatt
     2401 cgcggtaatt gaccgttaac caataagcat atactttggc tgcaccataa gggcttcgcg
     2461 gataaaacgg agtggtttct ttttggggta cttcttgaac aaggccgtac atttccgaac
     2521 ttgatgcttg gtaaaaacgc gccttgcacc cggtcatacg gattgcctct aaaatgcgcg
     2581 cagttcctac acccgtcaca tcccccgtat attcgggaat atcaaaggaa acgcgaacat
     2641 gcgattgagc tgccagatga tagatttcgt ccggctcgat ttgatgaatc aatttgatca
     2701 gattggccga atcggctata tctccataat gcaaaaacat gcgcacgcca ttgacatgcg
     2761 ggtctttata caaatgatct atgcgatcgg tattaaaagt cgaagcgcgg cgaataatac
     2821 catggacttc gtatcctttt tcaagtaaga gttccgccag atatgaacca tcctgaccag
     2881 tgatacctgt aatgagagct tttttattca tgacactgat gtctccctat ccatcactta
     2941 atgcaaaatt aactaatttc atattgttat ttaacatgaa gaattttcat gactttttga
     3001 aatttttttc gagaaacaaa tgaagaagct tcaaatctaa caaaatacac cgcactagac
     3061 tttgccgttg caaaccattt cacctcataa tatttaggct caaccttttc cagtacaatt
     3121 ctatctacaa attggccaat tgtattataa aaagtaatag ttacattaga aagttcaggg
     3181 atttgaaaac aaatattggt catatgtcca aacggatttg gataattttg atgtagttta
     3241 taatctgtag gaacatctac ataattgcga tttgcatacg cttgagtagt aaaaaaggtc
     3301 accgatgaat ctgacatcga actgaattcg tcatctactg catataactt caaagaatac
     3361 tcggacatct cattcaaaac agcgctatca aagtcgagtg aagtatcgct tttgactttg
     3421 atcatagtat ccagattatt gcctgtaaaa tgtagcaaat aattaacagt atcctcgtta
     3481 tcgggatcaa tggatgcccg ccatatgaat ctgtaaacaa gcggctttct aaatatagtg
     3541 atagtatcgc ccatcgccgg gaaaacagca atgggtacag gaggcggata attcgttggt
     3601 tcgatgtgaa taacgatcat agtatcttta aaaccaccaa attgatcttt tgctcgtaaa
     3661 tttaacggca aatcaccatg agtattgttt ggaggcatac cgctcatttt tcctgtcaat
     3721 gaatctatct ccatccattt gggaccgccc agaatttcat attttactgt atcgccaaat
     3781 aaagcactat ctttatcaga tgcataaaca taagcatcat aaattaagcc ctctttggca
     3841 tccgttttaa attcgcttct ccaaaggggc ggatgatttg tgtggacaac attgagagtg
     3901 attgatgccg aatcataatc tccggcaaca tctgtcactt gcagttttat cacataactt
     3961 cctatatcgt ttgccgtagg tgtcccgtaa aaaaaacctc tcaccgaatc aagtataagc
     4021 cactgtggga ggttatcaaa tcgatatcgc aattcagatt catataacag tgtatcaggg
     4081 tcgtttacta aaatattaga atgaaaataa gcatcttcag ctgcattaaa aaaataatta
     4141 ctaataattt gcggtttatt attaagccaa aatcgaaggg tatcagatat atcataatca
     4201 aagttgagct gcgtattaag aagttcagaa cgagccaatt caggtccaaa aaaataagaa
     4261 ccccgtatag attcaaatcg acttcggtaa ccctcagtaa ctttgactct agtttcattc
     4321 atgatatcct gaatcaataa tggaactcta gacttagcaa taatggcgct cggatctatc
     4381 gtttttagta aattcgcttc atacaaatca ggataatatc catacgtagt attcaaaagt
     4441 tgattccaat caaacagata attggataga atagttgttc cagtttttgg atatttattt
     4501 gcaacgaatt gcacaacaag cgatggagaa aaaccatacc cccacaatac ggacgagata
     4561 tccgcgcgat tatcttcaag atacttcaaa attgcgatca agctgctaat ccgatgtccc
     4621 attaataact ggccggaata accaacccat tccgttgtcc attgttcttt ataatagcct
     4681 gatccattta aactatttgt agaaggaaat tgtatatcca gttcaccata accgaggaga
     4741 tcaattccag taacaataaa acctgaatcc aataaatttg tcaattgtct cgaaaggctt
     4801 gagtcggtct tccatacttc acgacctatt tcattttcag caacaaaaaa aactttattt
     4861 ttaggccttt tatcatttct ctttaatgtt actaccggat actccagttt agaaccgtct
     4921 ctcaagggct cttttaaaac gatagtttca tataacacgg tatatgggcc actactcaat
     4981 gagtctttaa cagaaacact gattacagac caagatgtat ctacacccaa cttactatac
     5041 gcttctgaaa tatcataatc ctcaagatcg gataccgatt ttaaactgtt tgcgtaaatc
     5101 atctcggata agatttcatc cgtgtttttt gtttcaaaat cacttataag aaaagaagag
     5161 gggtgaaaca atgtatcaaa aagctcaatt gatgggtcat gttcattcaa acctgcaggt
     5221 aaccccaata ttgtatcaaa atacgaataa gcaatcagcc ttttttcgtt attgtagttg
     5281 tgattgccta catttgtcaa aaaatgaaac ccggacgcgg tattatacat ttcatataat
     5341 tttttaaaac gatcaagcgc agcaggaata tagttttcat atagctcttg atttgaatca
     5401 ataaaaatca tagggcgagg tggaaataaa gaaaataaaa caaaatcatc tatgcttgct
     5461 tcagaacctg aaaacgttgt agatctagga tctatgcgtt cagattcatt gatcaattta
     5521 gttttatata tcccaccatc atttacaata gtacaagcta ttctttgatc tatagcgcct
     5581 acttcgacag caatagcccc agcggccgaa tatcctgtta taccaataaa tgtggtatct
     5641 acatcttcac gcgaaaaaag atagttaatt ccctgaataa aaggagctaa acgagaacga
     5701 accttaaagg catctccagc aaaattaaac ttataaccct gatactggta agtatctccg
     5761 gctaaggcgg attgaccaag cccaagctga ttaatactta gcactacata accgcgcaga
     5821 gctagatgtt gcgacatgcg ctgccaatga tcaacatttg caaaattgta accataggca
     5881 tcaaagtagt acccttcggc atacaccact gccggttttt tttctagtcc attctttgga
     5941 agataaagag ttgccggcac tctgactcca tcataagact gaaaagttaa aaggacaatt
     6001 gaatataacg gtctatctag tgtatcatga taaatcgcat tgagcggata ttgagcagaa
     6061 agccattgat cgagcttgta aaattgcttt aacgaatcaa cgatgcgatt tcgccgagac
     6121 tcccattcga ccaacgttga tggtactatt aattcgttaa aaccactgag aaaatagttt
     6181 tctagcgact cttttttatc ggaagagcca tttaaataac tatagttatc aaaaatcttt
     6241 ttagaaaaca attccaaagg cacaacttcc tgtgggcaaa ccctggaaaa aggaaacaga
     6301 agtactacgg cgaaacagat atataaaaaa acagaagaag tgatgccgcg tgtatggata
     6361 tatcccctgt aattttctgt catattcttc acattttgct ttataagcgg ccaaaacggc
     6421 ggtgaagtaa atagataaat atatcgtgca ttccaagtat aaagaaacga ggaacaacgt
     6481 acaaatatct cttccacaag cgacgaggct ccacacacaa tcgaaaaaac cactcaaggc
     6541 cgagtttttg tacccacaaa ggcgcttgtt ttatattgcc tgtatgaaag tcaaatgccg
     6601 ctccaatcgt aactaaaaaa tgagtgccaa tatatccgga caacctccgt gcaaaatact
     6661 cttgcttcgg actacctaaa cctatccaaa caatatccgc cgatgtatca ttgatctgat
     6721 ttgctaagtc tcgcaactct tcatctgtca tatcacgaaa ggggggggaa taacaaccga
     6781 caacatggtt gttgccaaat ctatcactac aaacacgtcg aagttgttca gcaatacctt
     6841 gtttacctcc acacaaaaaa tgacgcactg gtgttgtact tgaagtttgc atgactaact
     6901 taaaaaaatc agggccgtaa caacgaccca tattggaatg tcctttgatg cgaccaaccc
     6961 aaactgtagg cattccatca gggagcctta aataaaaaga ttctaataat tctttgaact
     7021 cgcgttcact atgtgaccgc actataccat gagcatccgt agcagaaacg attcgattag
     7081 atttctgaat cgtcttgcat tcatttaata taacatttac ggctctatgt atcccttgat
     7141 cataaaactc aattccgaca attttggcca ttaatcaccc gacatttcat attgcatatt
     7201 tataaaacgt attgcctctt gaaaaccggc ttgcattact tgaattcgac aagcactagc
     7261 gattttttca tctatttttt tcttcattcg ttcccgctct ttgtcctcca ccaaaaactt
     7321 atacaaccct tcagtaaaac gatcaatatc atgatctaac aaaaggccag tctcaccatt
     7381 ttctaaatat tcaatttctg tactatgaaa tggccccaat ggcatggtct ttttagaaat
     7441 aactggacat ctaaaacaaa atgcatgtac aatagataat cccaaaaaac ccggcattac
     7501 aaatgcatca cttgcatata aaaaaggtcc taattctttg ttatctaata tttctccata
     7561 cattttcaca aaaggcttca tttcatattg atcgataagc gcctgcatat tgctttgtaa
     7621 gctcccacgc ccaatcacat gtaaatgaac atccaaaccg gaaatgagta actttctcat
     7681 tacttcgatc aaaaaaagcg gttccttatc ctgaacaata cgcccgatat atattaaatt
     7741 atatttcgca gtaaatccta ttttacgctt aatcacatca aagtcgtcac gttgaagtga
     7801 caaatactgt tgaaaaagaa catctgtatt aagggtatta ttagcaacaa acatcttacc
     7861 ggctatctcc tctgaaggca agacggaaag aatatctttc tttcgattag cattataaaa
     7921 taatacggca tcagcttggc gcatcatata gagccgcagt ttatctttag aacttctacg
     7981 gggatcaaat atctcttttc tgtcataacc gtggccccac aatacaatgc gaatgccata
     8041 tagtttacga attaaaaaca ataaccagat agaaaccata cttaattcaa attgaataaa
     8101 gacgaaacgc ggtttgtatt ttaggatcgg agtaacaaca tccataaaga taatattctc
     8161 gcctgtccaa cccttattcc gaaagtaacg cacggttatt gaacttgttt tttcggctag
     8221 atgcgaattc gcagaactac tcgacaatcc atcaatctga tcagagcaat gtatccatcg
     8281 gaaggacatc acttcagcta attttccaag aatctctgct cgatagtgtg gtagcgtccg
     8341 gtgaaatatc agggcatcag ttttcatata aaattttttt atttatactt aagaataaaa
     8401 caaatgaaaa aataatactc atatcactaa gcacattggt ggtaatagtc aaaccaatca
     8461 tacaaaaaga aaacaagata ggtataaaag caaccctttt atcggaaaat tcacgagcaa
     8521 tagaatatat caccatatag cctgccccca gtcctaataa accaatttca gaatagaatg
     8581 taaaaatcag gccgttattg ccgacaagat attcttgagt aacaggatca taatattttg
     8641 atggtccgtc accaaaaaat ttcaatggct cattcaaata ataaagaacg gctgcttcgc
     8701 gtgagtacct tcctgcagca aaattctcat aacttgagta ttcatccaca tcgaaggtta
     8761 tgcgccaaac aagatcctga aagttttcaa gtaattgatc taggaagcct gaaaagtata
     8821 aactaataac aatgaacacc aacaataccg cacctgacaa aatattcttt aacgacattt
     8881 tcattagaaa gaatgctaga agaaccaaag caagaagaat atgcgccatg cgtgcgttag
     8941 ttatcagaat agtaaaagca aagtagccaa cccaaaaata tggtaatgtt atatactgat
     9001 gcgac
//
LOCUS       DAOQNQ010000116         8972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2868_length_8972_cov_6.044185, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000116 DAOQNQ010000000
VERSION     DAOQNQ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8972
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2868_length_8972_cov_6.044185"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PK754_08450"
     CDS             complement(<1..637)
                     /locus_tag="PK754_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09573.1"
                     /translation="MNQQWRRGFFLLLIPLLSAFSQNQAVLRSVAVLRFELRSASTEN
                     DWLGFGFAETLTSKLGMVDGLMVMERTKLHELLNIAGKSEPLDETQVSAGEEYARIIG
                     AEYLLIGSVQVVGSYKNTSTAIKVNARIIKTSSAEITGNMAFSVSGTVQEIFELQTQL
                     ATRFCEVIGAPAGLAELRYEDAQSIDAQQWYGRGLQAFYESNYAQAITCFRR"
     gene            complement(680..3250)
                     /locus_tag="PK754_08455"
     CDS             complement(680..3250)
                     /locus_tag="PK754_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNB09574.1"
                     /translation="MLMQTRFLKPRLKNYAMGVWCMMAGWCAPALVQTQSLKSVAVIR
                     FDNRSPLPENNWLGIGFAETLTSKLGMVDGLMVMERTKLHEILTAAGKTEPIDENRTE
                     VGLAYAKMIGVEYLLIGSVQVTGSYKNPASQLRINARMIKTSTADISGNLTFSANGNI
                     KDIFAIQTELAEKFCTALGVTSGLAGLTFEDAQSIEAQQWYGRGLKAYYEANYAAAIE
                     LFRSAVNNNEGVFYAAAHTMEGYARQKQIERAQDAEKQTYKQAYLDQFRRDAGEAAPA
                     FYDLGIAHQATGLYDDAVKSFGEYLRYMDKASQLVTECAPEVENPRRIQTVEPDSNEP
                     YDIFRPAVQKGFYYRLSKGSNYETNGVECVWNNGAVRWRSKLPDCGGESPQLNKMDLF
                     GDTLYMGAYVIAMVNNKTGKAYWQFPERPFLNDIGWPLLIEDVAFYKDKMYISANNTI
                     YIINRSTGKKLAEYRGGSGVYVKEGIFNIGANCVNVDRLLEQKLYTEVDALHQISKTY
                     LLAQRLDSALKYAEAVMQSYPNHIGANEITAHVALAQGQRKTAVDRIIQLYTLTPNHT
                     LRDIARSEFGILGVTTFHGWLATLKCDRDALWAVFGGEFNRGANVNELWRIDAVTDQT
                     VWRKAMDYEGQYTLSYDADSSYIYCAWPRKAWENNAETWKYDLLCLSKKNGQVVWKTL
                     LRDQCQVTEGVYSSSIVPRIDGDFLYITFYNGNVYKVFRSNGAIVWGRSCFGDGTIRR
                     YEANEEYFTRCNLGIVSQVVYVHVPMPSVQRDTVIGFDKITGQRRLVLPEMREFRAAM
                     PEDTTATVLFSGYGSYGYYPESVFTEKKIYAGSKDQGILILDRAILEKLRADKKIWWH
                     "
     gene            complement(3527..6730)
                     /locus_tag="PK754_08460"
     CDS             complement(3527..6730)
                     /locus_tag="PK754_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNB09575.1"
                     /translation="MRIWDFAVDNKVTVYILMMIIVILGTMSYSSLPREAAPDISIPL
                     VIVSVPYVGVSPADMEGLVTQPMEKELKSLKDVKEITSSSKEGLSTIRVEFETGIDID
                     EALRRVRDKVNQTKPKLPSDILEPIISEINFSEFPIMFVTLGGDLGLARMKRIAEDLQ
                     DKIEAVPGVLSSDITGSLEPEVQVNCDINRLKGYSVSFDDVINAIRSENLTTPGGAIN
                     NGTTEYSVRIPGEFAAPKPIEDLIIKMRHGSPIYVRDVATVEYLFEDRSTYSRLNGSP
                     VLTLSVKKRAGENLIQIADEVKTILSDEKANFPPGLHVRITNDASIDIKRSVKELENS
                     VVTGMVLVVLVLFMFFGMKNSFLISTSIPISMLIGFVILSFMGVTLNFVVLFALVLVL
                     GILVDDAIVVIENIYRHQHEYGKNPIQAAKDATREVSIPVATSTFTTISGFLPMLFWP
                     GVIGDFMYYLPLTLIIMMGASIFTAYVISPTQGAQFIDYHKEMKALNSSMQQKHWWTH
                     YNPFSRLYHYVDTHFFPRAQEKYVKTLQLTLRHKTMTVVLSGVLLFAMTFLFVAFSKG
                     VEFFPETQPNQVSVNIAMPSGTPLEMTNAVTAMVEERMKHVEGVKDMEFVVANVGTSE
                     DPFDFGGQGTPNKSQISINFYEKMKREKNTFETMESMREHAKGIAGAEIKVKGQENGP
                     PVGAPVSIDLSGDDFRMLAQLSEKIQSEIRSIPGLVDLKDDYDAGKPEIQVIVDREKA
                     ALLEMSTGQIAMAVRTAINGTEASKFRVGEDEYKITVRLREDQRQSPSEIENLNITFM
                     NNRGVLMSVPLASIATVVHSNGLSNVRRKDLRRVITISGDVQNRLANDVLHDVREKLK
                     TFKLPNGYRIEYTGENKEMDKAMAFLAKTLVITILLIFLVLVSEFNSVKVPMVIMVSV
                     PLSLIGVFLGLLLTGTPFGVIMTGIGVISLAGIVVKNAIVLLDFTKHLRGSGMTLDEA
                     LAEAGRTRLRPVLLTAATTILGILPLASGIDFDWREWHFIIGAESSDFWRPLGVAVIF
                     GLTVSTFLTLVIVPTTYSWVEGITKAVGRWFKRETTV"
     gene            complement(6758..7807)
                     /locus_tag="PK754_08465"
     CDS             complement(6758..7807)
                     /locus_tag="PK754_08465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB09576.1"
                     /translation="MKIHQRLSLLLLPGYLWVTGCGSSNAGPGDTKKEPEAIVYIKTE
                     KIIAKPFAETIRLTGTVESVDNIIVPAEEGGRILRWLATKGATVQRGQAIAQIDDAAY
                     RSAYDAAQAAYQMAEVNYQKQKSAFAEQAISELQLKNLEFQRDAAKAQADLAKQRLDK
                     TTVTSPVNGVLNEQFADAGEMAAPGMPIAQIIDMTNLKVMVGVPERYARDVQPNLSVE
                     FTADAFPGETFRGKIGFVGAAVNPDNRTIPVELFLTNPGGKLKPQMIASVAMKLAAIE
                     KAILIQRDFVQQVDAGRLVVYVHKDGIAEERAVRIGGMSRDFVHILSGLQEGDEVITV
                     GYQNLTPQQAVRIQP"
     gene            complement(7830..>8972)
                     /locus_tag="PK754_08470"
     CDS             complement(7830..>8972)
                     /locus_tag="PK754_08470"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNB09577.1"
                     /translation="MPSITLDTRYTRALKKPVFFLPDFENPASGKVTPIEIGSNHSIE
                     TGFTATQALFNFAVFTGVGTAKVYQRTSRALYRSEYNRMVASVKKVFYQVLLARQVYE
                     TTKASLINAENNFNNVRILNKQGIVSDYDMIRAEVQVENLRPSVISAEQQALVVTNAL
                     KVQLGMDATDPLHIRGELTPVPIDSSWLDEQRAVTQNAGLEALRLQQDLNAAVSSIRL
                     AEYMPTLAAFGTYQWQAQKNTMDFGGKDFISTSQVGFTLSLNLFNGFQTTARLAQSRA
                     DERKAEQQLRYTRENIRSQMQSIRLRLDEARKRMASQNRTVELAEKSYKIATTRYATG
                     SGTQLEVNDADVALLQARLNRIHAIYDYLVTCVDLEEMISYHQPQN"
BASE COUNT         2260 a         2324 c         1994 g         2394 t
ORIGIN      
        1 cacggcgaaa gcatgttatc gcctgggcat aattcgattc gtaaaaggct tggagcccgc
       61 gcccatacca ttgttgtgca tcaatgcttt gcgcgtcttc atatcgtaac tccgctaatc
      121 cggccggcgc tccgataact tcacaaaatc gagtagccag ttgggtttgc aattcaaaaa
      181 tttcctgtac cgttcctgaa actgaaaatg ccatatttcc ggtgatttct gccgaacttg
      241 ttttgataat acgcgcattg actttgatcg ctgttgacgt gtttttataa ctgccgacca
      301 cttgcacgga tccgatcagc aaatattctg cgccgatgat ccgcgcatat tcctcacctg
      361 cactcacttg cgtttcgtcc aagggctcag atttaccggc gatgttgagt aattcatgca
      421 actttgtccg ctccatcacc atcagaccat ccaccatacc caatttggac gtcagagttt
      481 ccgcaaaacc aaatcctaac caatcattct ccgttgaagc cgagcgaagt tcaaaacgaa
      541 gtacagctac cgacctgagc acggcttgat tttgtgaaaa agccgaaagg agagggataa
      601 gtaaaagaaa aaaacctctg cgccattgtt gattcatcat caagacctat ctgatataac
      661 ttagtttcat tttttgattt tagtgccacc aaattttttt atcggcacga agtttttcta
      721 aaatagcacg gtctaatatc agaataccct gatcttttga acctgcatat attttttttt
      781 cagtaaagac ggattccgga taataaccat aactaccata tccggaaaat aaaaccgttg
      841 ccgtcgtatc ctccggcatc gcggcccgga attcacgcat ctcaggcaat acgagacgcc
      901 gctgccccgt gatcttgtca aatccgataa ccgtatctct ttgcacggat ggcatcggta
      961 catggacgta caccacttgg gaaacaatac cgagattgca tcgcgtaaaa tattcctcgt
     1021 tcgcttcgta ccgcctgatt gtgccatcgc caaaacaact gcgtccccag actatcgcgc
     1081 cattggaccg aaacacctta tagacattgc cattgtaaaa cgtgatgtaa aggaaatcac
     1141 cgtctatacg cggtacgata gaggacgaat aaacaccttc ggtcacctga cattgatccc
     1201 tgagcagagt tttccaaacc acctgaccgt ttttcttaga cagacaaaga agatcgtatt
     1261 tccatgtttc tgcgttattt tcccacgctt tgcgcggcca agcgcagtat atgtacgacg
     1321 aatccgcgtc atacgataag gtatattgcc cttcatagtc catcgcttta cgccatactg
     1381 tctggtcagt aaccgcatct attcgccata actcattgac attggctccg cggttaaact
     1441 ctccgccgaa tacagcccac agagcatcgc gatcacactt gagtgtggct aaccatccat
     1501 gaaacgtcgt aacgcccagg ataccaaatt ccgaacgcgc tatatcacga agcgtatgat
     1561 tgggcgtcaa tgtatagagc tggatgattc gatccaccgc agtttttctt tgcccctgtg
     1621 ccagcgccac gtgcgccgtg atctcgttgg caccgatgtg gttgggatac gattgcatca
     1681 cggcctcggc atatttcaat gcagagtcca aacgttgtgc taagagatat gttttggaaa
     1741 tctgatgcag cgcatccact tcagtatata atttttgctc cagcagccgg tccacattaa
     1801 cgcaattggc gccgatatta aaaatgcctt ctttcacata gacgccgctg ccacctcggt
     1861 actcggccaa tttttttccg gtagatcggt tgataatgta gatggtatta ttggcgctga
     1921 tgtacatttt atctttataa aacgcgacat cttcgatgag caacggccag ccgatatcat
     1981 tgagaaacgg ccgctcggga aactgccagt acgcttttcc tgttttatta ttgaccatag
     2041 cgatgacgta cgcgcccatg tatagcgtat cgccaaacaa atccatttta tttaattggg
     2101 gactttcacc tccacaatca ggtaatttgc ttcgccaccg tacagcgccg ttattccaaa
     2161 cacattcgac accgttcgtc tcatagtttg atcctttgga gaggcggtaa taaaatcctt
     2221 tttgaacggc aggacgaaaa atatcgtagg gctcattgct atccggctcc accgtctgaa
     2281 ttcgtcgtgg attttctact tcgggtgcgc attctgttac caattgactg gctttatcca
     2341 tatagcgcag atattcaccg aaagatttaa ccgcgtcatc gtataatccg gtcgcctgat
     2401 gtgcgatgcc cagatcgtaa aaagccggtg ccgcttcacc cgcatctctt cgaaactgat
     2461 ctaggtaggc ctgcttgtat gtttgctttt ccgcatcttg cgcacgctcg atctgctttt
     2521 gacgtgcata accctccatg gtgtgcgcgg cggcgtagaa tacgccttca ttgttattga
     2581 cagcgctacg aaatagttca atcgctgcag catagtttgc ctcataatag gctttcagcc
     2641 ctcggccgta ccactgctgc gcttcgatgc tctgagcatc ttcaaatgtc agaccagcca
     2701 agcccgatgt cacacccaaa gccgtgcaaa atttttcagc caattcggtt tgaatcgcaa
     2761 aaatatcttt gatgttacca ttggccgaaa atgtcagatt gccggatata tccgcagtgg
     2821 aagttttgat catgcgggca ttgatccgaa gttgacttgc agggttttta taactgccgg
     2881 ttacctgaac cgatccgatc agcagatatt ctacgccgat catcttcgca tacgctaaac
     2941 cgacttctgt gcggttttcg tctatcggct ctgtttttcc ggcggccgtc agtatttcgt
     3001 gaagttttgt tcgctccatc accatcagac catccaccat acccagctta gaggtcagtg
     3061 tttcagcaaa gccgattcct agccaattat tttccggtaa tggagaacgg ttatcaaaac
     3121 gaatcaccgc cacggatttg agggattgcg tctgaacgag ggcgggcgca caccatccgg
     3181 ccatcataca ccatacaccc atggcataat tttttaatcg cggttttaaa aaacgcgttt
     3241 gcataagcat ttcccgctcc ttcaggacgt gattactgtg ggttagaatt aaccccgttc
     3301 tttaatggga tttgatatgt cagtgtatat atgaaccgat actcaaaaaa taacaaaagt
     3361 caggtttaga taaagttatt tttttggaaa tttatagaat atcccgaatg aaaggttaat
     3421 tcatgaggca aacggctccg atagaatgcc gcaaaaaaaa tcggtattca tgttttttga
     3481 catcaatacc gatgatttta taatgcctac ttatatacgc aaatatttac actgtcgttt
     3541 ctcttttaaa ccagcgaccg acggctttgg taatgccctc tacccaagaa tacgtcgtcg
     3601 gaacgatcac gagcgtaaga aacgtagaaa cagtcagacc gaaaatgacc gccacaccga
     3661 gcggacgcca gaaatcgctg ctctccgcgc cgatgatgaa atgccattcg cgccaatcaa
     3721 aatcaatacc cgatgccaaa ggtaaaatgc cgagtatcgt cgtggccgcc gtgagcaaaa
     3781 caggccggag ccgtgttcga ccggcttcgg caagcgcctc gtccagcgtc ataccgctgc
     3841 cgcgtaaatg tttggtaaaa tccagcagca caatggcgtt ttttaccacg ataccggcaa
     3901 gcgaaatcac accgatcccc gtcatgatca cgccgaacgg cgttccggtc agtaacaaac
     3961 cgagaaacac accgatcaat gaaagcggca ccgataccat gatcaccatc ggaaccttca
     4021 cgctgttaaa ttcggacaca agcaccaaaa aaatcagaag aatcgttatc accagcgttt
     4081 ttgctaaaaa cgccatcgcc ttatccattt ctttattttc accggtgtat tcgatccgat
     4141 acccgttggg caatttaaat gttttcaatt tttcacgcac gtcatggagt acatcattgg
     4201 ctaagcgatt ttgtacatcg ccggaaatcg tgatcacccg tcgcagatct ttgcggcgta
     4261 cgttggataa gccgttgctg tgcacgaccg tagcaatcga agccagcggc acggacataa
     4321 gcacaccacg gttattcata aacgtgatat tgagattttc aatttccgag ggcgactggc
     4381 gctgatcttc acgcagacga acggtgattt tgtattcgtc ctcgccgacg cgaaatttgg
     4441 aggcctccgt accgttgatc gccgtacgca cggccatcgc gatttgccct gtgctcatct
     4501 ccagcagcgc cgctttttca cgatcaacga tcacctggat ctccggttta ccggcgtcat
     4561 aatcatcttt caaatccacc agcccgggaa tggagcgaat ttcagactgt attttttcag
     4621 aaagctgcgc cagcatacga aaatcatcac cggataaatc aatactcacc ggcgcaccca
     4681 ccggcgggcc gttttcctga cctttcactt tgatctccgc gccggcgatt cctttggcat
     4741 gctcgcgcat ggactccatc gtctcaaacg tatttttttc acgtttcatt ttttcataaa
     4801 aattaatgga aatctgcgat ttattgggcg taccctgtcc tccaaagtca aacggatctt
     4861 ccgacgtacc gacattggca accacaaact ccatatcctt cacgccttcg acatgcttca
     4921 tccgctcttc gaccatcgct gtcaccgcat tggtcatttc caagggcgta cccgacggca
     4981 tggctatatt gacagaaacc tgattgggct gtgtttcggg aaaaaattca acacctttgc
     5041 taaacgctac aaacagaaac gtcatagcaa aaagcaatac cccggacagc acgacggtca
     5101 tggttttatg gcgcagtgtc agttgcagcg tcttaacata tttttcctgt gctctcggaa
     5161 aaaaatgcgt atccacatag tggtagagac gactaaaagg gttgtagtgc gtccaccaat
     5221 gcttttgctg catagaagaa ttgagcgcct tcatttcttt atgataatct atgaactgcg
     5281 caccctgtgt cgggctgatg acatacgcgg tgaaaatgga agcccccatc atgatgatga
     5341 gcgtcaacgg cagataatac ataaaatcac cgattacacc cggccaaaaa agcatcggaa
     5401 gaaaaccgga gatcgtcgta aacgtcgaag tagccacggg aatcgacact tcccgtgtcg
     5461 catccttggc ggcctgaatc ggatttttac cgtattcatg ctgatgacgg tatatatttt
     5521 cgatgacgac gatggcatca tccaccagaa tacccaaaac caacaccagt gcaaacaaaa
     5581 cgacaaaatt gagcgtaaca cccatgaagc tgagaataac aaatccgata agcatcgaaa
     5641 tcggtattga cgtgctgatc agaaacgaat ttttcattcc aaaaaacatg aagagcacca
     5701 gcacgaccag taccatcccc gtcactacgg agttttctaa ttccttcacg gaacgtttga
     5761 tatctatcga agcatcattg gtgatacgta catgcaggcc gggcgggaaa ttggcttttt
     5821 catcggacaa tatcgttttc acttcatcgg ctatctggat aagattttca cctgcgcgtt
     5881 ttttgacgct taacgtaagc acaggtgatc cgttcagacg actataggtt gatcggtctt
     5941 caaacaaata ttcgacagta gccacatcgc gcacatagat cggggaaccg tgcctcattt
     6001 ttataatcag atcttcgatc ggtttaggcg cagcaaattc gcccggaatg cgcacgctgt
     6061 actccgtcgt cccgttattg atcgcaccgc cgggcgtcgt aagattttca ctgcgaatgg
     6121 cattgatcac atcgtcaaaa ctgacgctgt agcccttgag gcgattgata tcgcagttga
     6181 cttgcacctc cggttccaga ctgcctgtaa tgtccgaact cagcactccc ggcaccgctt
     6241 cgattttgtc ttgcaaatcc tcggcgattc gcttcatacg cgccaatccg agatcgccgc
     6301 cgagcgtgac aaacatgatc ggaaattcgc tgaaattgat ttcactgatg atcggttcca
     6361 gaatatccga cggcagcttg ggtttggtct gattgacttt gtcacgcaca cgacgcaagg
     6421 cttcgtctat atctatcccg gtttcaaatt cgacacgtat cgttgacagc ccttctttgg
     6481 aagacgacgt gatttctttg acatccttca gcgatttgag ttctttctcc atcggctgtg
     6541 tgacgagtcc ttccatatcc gccgggctta cgccgacgta aggaacggaa acgatcacaa
     6601 gaggaatgga tatatccggc gcggcttccc gcggaagact gctgtatgac atcgtaccca
     6661 gaatcacgat gatcatcata aggatataca ccgtgacttt attatcgact gcaaaatccc
     6721 aaattctcat gggaaaattc cttgaagcta agtttcgtta tggctgaatg cgcacggcct
     6781 gttgcggcgt cagattttga tagccgaccg taatcacttc atcaccctcc tgcaaaccgg
     6841 ataggatatg aacaaaatcg cggctcatac cgccgatccg cacagctcgt tcttccgcaa
     6901 taccgtcttt gtggacatac acgacgagcc ggccggcatc cacttgttgt acgaagtcgc
     6961 gctgaatgag aatggctttt tcgatcgcgg ccaatttcat cgctacggaa gcaatcatct
     7021 gcggtttgag ttttccgccg ggattcgtga gaaaaagttc taccggaatc gtccggttat
     7081 ccggattgac ggccgcgcca acaaatccga ttttgccgcg aaacgtttca cccggaaacg
     7141 catccgccgt gaattccacg ctgagattgg gctgcacgtc acgcgcatac cgctcaggta
     7201 cgccgaccat gactttgaga ttggtcatgt caatgatctg ggcaatcggc atgcccggcg
     7261 ccgccatttc tcccgcatcg gcaaactgtt cattgagtac gccattaacc gggctggtaa
     7321 cggtagtttt gtcgagacgt tgttttgcca gatcagcttg tgctttggcc gcgtcgcgct
     7381 gaaattcgag gttcttaagt tgcagttcag atattgcttg ctctgcaaac gcgcttttct
     7441 gtttttgata attgacctcg gccatctggt aagccgcttg cgctgcatcg taggcggaac
     7501 ggtaagcggc atcgtcaatc tgtgcgatcg cctgcccgcg ctgaaccgta gcgcccttgg
     7561 tggcaagcca ccgcaggata cgtccgcctt cttccgccgg tacgataata ttatctacgg
     7621 attctacagt tcccgtaagg cgaatcgtct cggcaaaggg tttggcgata attttttctg
     7681 tctttatata gacgatcgct tccggttctt tttttgtgtc gcccggaccg gcattggatg
     7741 aaccgcagcc cgtcacccat aggtaacccg gtaaaagcag aaggcttaat ctttgatgta
     7801 tcttcataac agctccgtcg ctggtaaaat taattttgcg gttgatgata actgatcatt
     7861 tcctcaagat ccacgcaggt cacaagatag tcgtaaatgg cgtgaatacg gtttaggcgt
     7921 gcctgcaata aagccacatc agcatcgttg acttcgagtt gtgtacccga tccggtggca
     7981 taacgcgtag tagcgatctt gtagcttttt tcagccagct ccaccgtacg gttttggctg
     8041 gccatacgtt tacgcgcttc atccaaacgc agacggatgc tttgcatttg actgcgaatg
     8101 ttttcgcgcg tgtagcgcag ttgctgttcg gctttgcgtt catccgcgcg ggattgggct
     8161 aatcgcgccg ttgtctgaaa accattgaac agattgaggc tcagcgtgaa gccgacttgc
     8221 gatgtactga taaaatcttt tcctccaaag tccatcgtat ttttctgcgc ctgccattga
     8281 tatgtaccaa acgccgcaag ggtgggcatg tattcggcca gtcgtataga cgaaacagcg
     8341 gcattaagat cttgttgcaa tcgcagtgct tccaatccgg cattttgtgt caccgcacgt
     8401 tgttcgtcca accaactgct gtcaatcggt acaggcgtca gttcaccacg gatgtgcaga
     8461 ggatccgtgg catccatccc gagctgaact ttcagagcat tggtgacaac cagcgcttgt
     8521 tgttccgctg aaatcaccga cggccgcagg ttttctactt gcacttcggc acggatcata
     8581 tcgtagtcgg atacaatacc ttgtttattg agaatgcgga cgttgttaaa attattttcg
     8641 gcattgatca gactggcttt ggtggtttca tacacctgcc tcgccagcag tacctgataa
     8701 aaaacttttt taaccgaggc caccatacga ttgtattccg aacgatataa tgcccgcgac
     8761 gtccgctgat atactttggc cgtgccgacg cccgtaaata cggcaaaatt aaacaaggct
     8821 tgcgttgcgg taaatccggt ttcgatagag tggttggaac caatctcgat cggtgtcact
     8881 ttgccgctgg ccggattttc aaaatccgga agaaaaaaaa ccggtttttt gagagcacgg
     8941 gtatagcgtg tgtccagagt tatgctgggc at
//
LOCUS       DAOQNQ010000117         8967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2871_length_8967_cov_6.053972, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000117 DAOQNQ010000000
VERSION     DAOQNQ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8967
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2871_length_8967_cov_6.053972"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(290..763)
                     /locus_tag="PK754_08475"
     CDS             complement(290..763)
                     /locus_tag="PK754_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNB09578.1"
                     /translation="MHEKTTKITDFDELDRKILNLLMENAQMPYTEIAEKVHTSSGTV
                     HVRMRKLESAGVVTGSKLRVDPAMLGYDLCAFLGIFLEKGSLYKKVVKQLEAIPEITE
                     LHYTTGNYNILAKLYCRDTRHLRDVLNDKIQTIEGVERTETFISLEPSLERAIRI"
     gene            complement(1111..1644)
                     /locus_tag="PK754_08480"
     CDS             complement(1111..1644)
                     /locus_tag="PK754_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09579.1"
                     /translation="MIIKLGLNASYPQMNGKTSLHSKKNATEAFLWSAGHTVLPVVAG
                     GYILNKLDTYETAGSFAVGYGFLIGPSMGNFYAQDFGRGLLGVGIRTAGTALFYSGVA
                     DALQDDWFDDEGNDSRGDGGAKITGGFIIYTIGALYSIFTLPQSVHDYNKKFELSASP
                     SYDPKSQTLSLHAQLVF"
     gene            complement(1882..2784)
                     /locus_tag="PK754_08485"
     CDS             complement(1882..2784)
                     /locus_tag="PK754_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09580.1"
                     /translation="MESFWPETPLLNAIPAQTITLSDLPVRSVISPDGDYVIVYDDST
                     LVVYNTISWRIETKMNMDSGLRRVCYGADGHHIALETSAGREIWDTKTWEVLYKEENA
                     DSLSIVLPSSQGFAKAKTQVEGDDWALIVQSWPYRQQFAKLNAPEERPLWTMDITPHL
                     IAMYHHDQKKITITEIKTGRIVDTLLAKRPDKIKLSPNGRYLAQIYNGAVRVYDRWSN
                     EEKYYGQRIIFGEPAGIFSADSNYVLIAGNGNVKMIELATGEVRLDLATRFHMVRSLE
                     LSKDNKLLTITDADERSVTVYRIG"
     gene            complement(2935..3849)
                     /locus_tag="PK754_08490"
     CDS             complement(2935..3849)
                     /locus_tag="PK754_08490"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HNB09581.1"
                     /translation="MNKVYTNAESIDFRLADIPERPEPEKLLMCSPDHFNVTEAINNF
                     MTDTAGQMNQVDRPLAFQQWTNIRFLYEALGYRVHTIRGEVGLEDMVFSANQSFPFWD
                     RHQEKPAVIMSNMRKDKRKGEVPYFQSWYRKQGYMIHELKGGCFESNGDALWFPEKHL
                     IISGFGSTTHHRTDLLALEQVARITDGPVVGIELKHPDFYHLNTTLAIIDKDVCLVYP
                     PGVGDHGMAILRLFFKTIIEVSKEEAYAPNFACNAFSPDGEIVFIQGTCVETIHALEK
                     LGKRVIGVDTSEFIKSGGSVFCMKIKVY"
     gene            complement(3906..4247)
                     /locus_tag="PK754_08495"
     CDS             complement(3906..4247)
                     /locus_tag="PK754_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB09582.1"
                     /translation="MPEFTANGKRYNNPVEFALGKIGGKWKIPILWRLKDRIWRYGEL
                     KRDLKKITHKMLSEQLDEMEADGLICREVFPEVPPRVEYSLTNKGRTVVPIIEALREL
                     GLAMMKSENIE"
     gene            4403..5191
                     /locus_tag="PK754_08500"
     CDS             4403..5191
                     /locus_tag="PK754_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HNB09583.1"
                     /translation="MKVFWIAVLINTVCVFGQHDPNTSSPKKLILMVAANSSVSAQTG
                     WPIGVWAAEITHPYLAFIEAGYEVEIVSPNGGKIEFDNYSDPRDPSGYSAHDMISMGF
                     IHTPSHMALLEKTRRLDEINEKNYDAIFVCGGQSPMYTFIDNTKLHTLFAKFYESGKP
                     SAAICHGTCILLKAKLSNGQYLIEGKTWTGFANSEEDFADKAVGKKIQPFRIESEAGK
                     MNNTNFITHNAFRPFAIRDGHLITGQQQNSGTEAAKLVIEMLGY"
     gene            5208..5807
                     /locus_tag="PK754_08505"
     CDS             5208..5807
                     /locus_tag="PK754_08505"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNB09584.1"
                     /translation="MLFSKTRTEALSDGVFAIVITLLVLEIKVPDMHASGQSLTHALV
                     GLLPKIISWVISFAVVMIFWINHHRLFHSLTSVDARCMGLNGIFLMGLSFIPFPTALM
                     GEYIHEALAVSLFGWIMALTAGVLPILRSYASKAGLLDPMHDKQTIKTYNRRSYILGS
                     GAYAVAALLAWIEPIIAIVLYAVIAMYFIRPKHRHLKHV"
     gene            5877..6296
                     /locus_tag="PK754_08510"
     CDS             5877..6296
                     /locus_tag="PK754_08510"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNB09585.1"
                     /translation="MPLIKRSAQAHWAGTGKEGKGDLSTQSTVLNKTQYSFGTRFENG
                     VGTNPEELIGAAHAGCFTMKLSFVLNAMGLTAEALDTKATVSFEDGVVTQVHLELTGK
                     VPGCTAEKFQEAANDAKANCPISKLLNTTITLDAKLV"
     gene            complement(6344..7096)
                     /locus_tag="PK754_08515"
     CDS             complement(6344..7096)
                     /locus_tag="PK754_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNB09586.1"
                     /translation="MNVLIIEDEKPAGERLVAMVHDYDPAIRIVATLHSVKEARGWFA
                     KHNAPDVILADIQLNDGLSLEIFKDTAIHAPIIFTTAFDEYLIKAFEFNSIDYLLKPI
                     QKPKLFHALDKYVRLRQHFTGSLSDLLAQMSRSSEPSLQRIVVQKGTDFIAVPINDVA
                     YFFTEHKMVFLVDARGKKFIVDKPLADLEAALDKQTFFRLNRKYIARISAIVRFKPYE
                     KGKIKIELQPGTSEDIIVSQENAADFKNWMGK"
     gene            complement(7111..8181)
                     /locus_tag="PK754_08520"
     CDS             complement(7111..8181)
                     /locus_tag="PK754_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNB09587.1"
                     /translation="MEPLNEENIDQELNDRTIRVIMIPFFGIVIPNITGMIDNSAYTV
                     NQLVLHYLYFMFIAFAIWHGNRYLLFRLRIRFSWLSQPMYKVLALMVSNMFYTVPIAV
                     GLMTAWYVFAQHRSADWDVIFKATALCVVCVVFITHTYETVFLIKSWETDKTKSEALE
                     KAKAQAELEALKNQIDPHFMFNSLNTLSHLIEQDPPKARMFNDNLAEVFRYILYNRNR
                     NLVLLSEELSFAEKYFSLLKIRFGESIGLHIAEDNVSPDNVLIPPISLQILLENAIKH
                     NTFSDQQPMAITIRVSDQAVVVNNPIRKKVQVKNSSRTGLENLNERYKLIIGQTIYIE
                     ENVENFTVHLPVLMTGAHAVPY"
     gene            8375..8791
                     /locus_tag="PK754_08525"
     CDS             8375..8791
                     /locus_tag="PK754_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HNB09588.1"
                     /translation="MDMASAFNSLNWLAIIVASISAFVLGGIWYSPLMFGNAWMKVAG
                     MTEESVKQGNPGKIYGGAFLLTLIAAINLGMFLGAQSDMAFGIAAGAATGIGWVAPAF
                     GVVYLFEQRPTLQWFINGAYWVVTFIVMGAIIGAWH"
BASE COUNT         2570 a         2006 c         1808 g         2583 t
ORIGIN      
        1 gataattgga tgtgtccggt atcacgtcgg tttaattttt tattcttttt catatccgta
       61 tcacctttgc tatttgctat atatgtatta gcatagagcg tgccaggtat atgaaaattt
      121 ataagaatct gttttttatg aaacgttatg aaatcctgcc aacaaaaaaa tgcaataaat
      181 cgaacattta atattgcagt gtagcttttt cactacacta cacaatcaga aatctgaaaa
      241 ctcaatccgt attccgatac tttatctgtg cgttccgtca cattctatat caaatacgga
      301 tagctctttc taaacttggc tctaaagaaa taaaggtctc ggtacgttcg acgccctcga
      361 tagtttgaat tttatcgttt agtacatctc tgagatgacg cgtgtcccgg caatagagtt
      421 tggctaaaat attataatta cctgtcgtat agtgaagttc agtgatttcc ggaatagcct
      481 caagctgctt caccactttt ttatacaacg agcctttttc aagaaaaatt ccgagaaacg
      541 cgcacagatc ataacccaac atggccggat caacgcgcag tttggatcct gtaacgacac
      601 cggccgattc caattttcgc atgcgaacat gtaccgttcc cgaagaagtg tgaacttttt
      661 cggcaatttc tgtgtagggc atctgcgcat tttccatcaa aagatttaat atctttcgat
      721 ccagttcatc aaaatcggta attttagtgg tcttttcgtg cataatttta caaaaaatta
      781 aaaaaataat atattttata caatatatgt aataatattt aaaataaaat aaattatttg
      841 taataatata cactttatat tacattattt acgttaataa tagattattt ctgttctttt
      901 tataattcaa atattgtagg gtgatgaaac aggcagccat accgtccggt ctcgacggtg
      961 gagaatttgg gataaattcc gattaccggg actaactact ccgtggaggt tcgactcctt
     1021 cccctacagc tcgtacacca ccataataaa aaactgttcc gacggtcacg gaacagtttt
     1081 tttgttatcg acttttgggg ttaattagaa ttaaaaaaca agttgtgcat gaagcgataa
     1141 agtctgggat ttaggatcat acgagggact cgctgataat tcaaattttt tgttataatc
     1201 atggacagat tgtggtaaag taaaaatgct atacaaagct ccgatcgtat aaataatgaa
     1261 cccgcctgtt atttttgcgc cgccgtcacc tcgactgtca ttaccttcgt catcaaacca
     1321 atcatcttga agcgcatccg caacaccact ataaaacaaa gcagtaccag cggtgcgaat
     1381 accaacacct agaagacctc gcccaaaatc ttgcgcataa aaattaccca tcgaaggtcc
     1441 aattaaaaaa ccgtagccca ctgcaaagct tccggccgtc tcatacgtgt ctaatttgtt
     1501 aagtatgtat cctccggcaa caacaggtaa tacggtatgt ccggcgctcc ataaaaaagc
     1561 ttctgtagca ttttttttgg aatgaagcga tgtttttcca ttcatttgtg gatacgaagc
     1621 attcaatccg agtttaataa tcacactacg tgatgaatct gctggcatag cccaaagatt
     1681 taaaccaaaa aaacagctga taaaaacgat agttttcatt ttaataattc tccgataaag
     1741 agtttaaata cgtctgattt gtgtgaaaca aaccctatgc cgaaaaattg attaaataaa
     1801 acaaggaggg agatggaaaa taattttacg atcgagtaaa actgtatcta gtattcacag
     1861 acgcaataaa aagatggcac gttagccgat acgataaaca gtaacacttc tttcgtcggc
     1921 gtcagtaatg gtcaggagtt tattatcttt ggagagttca agcgaccgta ccatatgaaa
     1981 gcgtgttgcc aaatcaagtc ggacttcgcc tgttgcaagc tcgatcattt taacgttacc
     2041 atttcctgct ataagcacat aattagaatc tgcgctgaat atccccgcgg gctctccgaa
     2101 tataattctt tgcccataat atttttcttc attgctccag cgatcgtaaa cgcgaaccgc
     2161 tccgttataa atttgcgcta gataacgtcc attggggctg agttttattt tatcaggacg
     2221 ctttgcaagc aacgtatcta cgatccgacc ggttttaatt tccgttatgg tgatcttctt
     2281 ttgatcatga tgatacatcg cgatgagatg cggcgtaata tccatagtcc acaaaggacg
     2341 ttcttccggc gcattgagtt ttgcaaactg ttgtctgtac ggccaggatt ggacaatcag
     2401 cgcccaatcg tcaccttcca cttgcgtttt tgcctttgca aaaccttgcg atgaaggaag
     2461 tactatagaa agcgaatccg cattttcttc tttgtacaag acttcccagg tcttagtatc
     2521 ccagatttca cgccccgccg atgtttccaa tgcgatatga tgaccatcgg caccataaca
     2581 cacacgacgc aatccgctat ccatattcat tttggtttcg atgcgccacg aaatcgtatt
     2641 gtacaccacc agcgtcgaat cgtcatacac aatgacatag tctccgtccg gactgatcac
     2701 cgagcgtacc ggcagatcgg ataatgttat cgtctgcgcg ggtatagcat ttaacaacgg
     2761 agtctccggc caaaacgatt ccatcaaata tccgtatagc ggggcaagaa aaaataaaaa
     2821 taaaaaaaca ccacctaccg tgaacccaag cattctgcgc atcgaatgag tcataagaat
     2881 aacattatat gaagtttttt tgtacggcgt gccgcagatg cggacgttgc gtttttaata
     2941 cacttttatt ttcatacaaa ataccgaacc gccggatttg ataaattccg aagtatctac
     3001 accgataact cttttgccca gcttttccaa tgcatgtatc gtttccacac acgtgccctg
     3061 aataaataca atttctccgt ccggtgaaaa tgcattgcat gcaaaattag gcgcatacgc
     3121 ttcttcttta ctgacttcga taatagtttt aaagaatagc cgcaggatgg ccatgccatg
     3181 atcaccgaca ccgggcggat aaacgaggca tacatcttta tctatgatcg ccaatgtagt
     3241 attgagatga taaaaatccg gatgtttcaa ctcgatccca accaccggtc catcggtaat
     3301 acgcgccact tgctccaacg ccagcaaatc ggtgcggtga tgcgtcgtac ttccgaaacc
     3361 tgaaatgatc agatgttttt cgggaaacca taacgcgtca ccgttggact cgaaacagcc
     3421 gccttttaat tcgtgaatca tgtagccttg tttgcgatac catgattgga agtatggcac
     3481 ttcccctttg cgcttatctt tacgcatatt actcatgatc accgcgggtt tttcctgatg
     3541 gcgatcccaa aaaggaaaac tttgattggc cgaaaaaacc atatcctcaa gccctacttc
     3601 accgcgtatt gtatgtacac gataccctaa agcttcgtat aaaaaacgaa tattggtcca
     3661 ctgctgaaac gcgagcgggc gatccacctg attcatttga ccggcggtat cggtcataaa
     3721 attattaatc gcttcggtca cattaaaatg gtcaggggag cacatcaaaa gtttttcagg
     3781 ttccggacgt tccggaatat cggcgagacg gaaatcaata gactccgcgt tggtatatac
     3841 tttgttcata tcatcaaaac tagtttagtt gtagataaga tcacagatca tccgtaagtc
     3901 ccacgttatt cgatattttc acttttcatc attgccagcc cgagctcacg tagtgcttcg
     3961 atgattggaa caaccgtgcg gcctttattc gtaaggctgt attcgacgcg tggcggcacc
     4021 tcgggaaaca cttcgcgaca aattaaacca tcggcttcca tttcatccag ttgttcactc
     4081 agcattttgt gggttatttt ttttaaatca cgttttaact cgccgtaacg ccatatgcga
     4141 tctttgagac gccaaagaat cgggatcttc cattttcctc cgattttccc aagcgcaaat
     4201 tctacgggat tattataccg tttaccgttg gctgtaaatt cgggcatgtt ttttttgata
     4261 cgtaagtaaa acaataacac caatactcac tcaaaagtgc gtatcacacc caaaggtgcg
     4321 ttcttgacga acgaaagtta gctattatat ttatcgagta caaaacattt ttatgctcaa
     4381 caaaacgcac gaggagtcta taatgaaagt attttggatc gccgtattga tcaataccgt
     4441 ttgtgttttc ggtcagcatg atcccaacac atcgtcacct aaaaaactca tactaatggt
     4501 cgcagcaaat tcttcggtat cggcacaaac cggatggccg atcggcgtgt gggcggccga
     4561 gatcacgcat ccgtacttag ctttcatcga agccggttac gaagttgaaa tcgtcagccc
     4621 taacggcggt aaaattgaat ttgacaatta cagtgatccg cgcgatccga gcggctattc
     4681 ggcgcacgat atgatcagca tgggttttat tcatacgcca tcgcacatgg ccctgttaga
     4741 aaaaacccgt cgtttggatg aaataaatga aaaaaactac gatgcgatct tcgtatgcgg
     4801 cggtcaatca cccatgtaca catttataga caacacaaaa ctgcatactc tgtttgccaa
     4861 attttacgaa tccggtaaac cgtcggcggc gatatgccac ggtacgtgca tcttacttaa
     4921 ggctaaatta agcaacggtc aatatctgat cgaaggaaaa acatggaccg ggtttgcaaa
     4981 cagcgaagaa gattttgccg ataaagcggt gggtaaaaaa attcaaccat ttcgtattga
     5041 aagcgaggcc ggaaaaatga ataacaccaa ttttatcact cacaatgcgt tccgcccttt
     5101 tgccattcgc gacgggcatc ttatcaccgg ccagcaacaa aacagcggca cggaagctgc
     5161 aaaattagtc atcgaaatgc ttggctacta atacctttga gtacattatg ttattttcca
     5221 aaacacgaac cgaagccctc agtgacggcg tttttgctat cgtcattaca ttgctcgtac
     5281 ttgaaatcaa agtgccggat atgcatgcat ccggtcaatc gttaacccat gcgttagtcg
     5341 gcctgctacc caaaattatc agttgggtta ttagttttgc cgtggtgatg attttctgga
     5401 tcaatcatca ccggttattt cacagcctta cttcggtcga tgcgcgttgc atgggtctta
     5461 acgggatttt tcttatggga ttatctttta ttccatttcc gaccgcgctg atgggcgaat
     5521 acattcatga ggcattagcc gttagtcttt ttggatggat catggcgctt acagccggag
     5581 tattgcctat tcttcgaagt tacgcatcca aagcaggcct gttggatcca atgcacgata
     5641 aacaaaccat taagacatat aaccggcgtt catatatact tggcagcgga gcttatgccg
     5701 tcgcggcctt gcttgcctgg atagaaccta ttatcgcgat tgttttatac gccgtgattg
     5761 cgatgtattt tattcgcccc aaacatcgcc atttaaaaca cgtatgattt gcattgcacg
     5821 taataggcga cgatccgtat actatgccaa cttaggtcaa acaaggagaa ctagttatgc
     5881 cgttgattaa acgttcagca caagcccact gggcaggcac aggcaaagaa ggtaaaggcg
     5941 atctgtccac gcaaagcacc gtactgaata aaacacaata ttcgtttggg acgcgctttg
     6001 aaaacggcgt cggtaccaat ccggaagaat tgatcggcgc ggcgcatgcg ggttgcttta
     6061 cgatgaagct aagttttgta ttaaacgcta tgggattaac tgccgaagcc ctcgacacta
     6121 aagctaccgt ttcttttgag gacggtgtgg taacgcaggt acatcttgaa cttaccggta
     6181 aagtgcccgg ctgcacagcg gaaaagtttc aagaagccgc caatgatgca aaagccaact
     6241 gcccgatatc caaattgctg aataccacaa tcactttgga tgcaaaattg gtttaactcc
     6301 aaaacaatat gaatgtataa aaccacgctt cggcgtggtt tttttatttg cccatccagt
     6361 ttttaaaatc ggccgcgttt tcctggctca cgataatatc ctccgatgta ccaggctgca
     6421 attcaatttt gatttttcct ttttcgtacg gcttaaatcg aactatcgcc gaaatacgtg
     6481 caatatactt tcgattgagc ctgaaaaagg tttgtttatc caaagccgct tccaagtctg
     6541 ccaacggttt atcaacgata aattttttac cacgagcgtc aaccaaaaaa accattttgt
     6601 gttctgtaaa aaaataagct acatcattta taggtaccgc gataaaatcc gttccttttt
     6661 gaacgacgat gcgttgcaac gaaggctcgg aagaacgact catctgcgcc agtagatcgg
     6721 agagactgcc ggtgaaatgt tgccgaagcc gcacgtattt atcaagcgcg tgaaacagtt
     6781 tcggtttttg tatcggtttg agaagataat ctatggagtt aaattcgaat gctttgatca
     6841 aatattcatc aaacgccgtt gtaaaaatga tcggcgcatg gatcgccgta tccttgaaaa
     6901 tttccagcga taatccgtca ttgagctgaa tatccgccaa tatcacgtcg ggtgcattat
     6961 gttttgcaaa ccaaccccgt gcttctttta cactgtggag cgtcgctacg atacgaatcg
     7021 caggatcgta gtcatgcacc atcgcaacca accgttcacc cgcaggtttt tcgtcttcaa
     7081 taatcagtac attcatataa catggatcgt ttagtacggc actgcatgtg caccggtcat
     7141 cagtacaggc aggtgaaccg tgaaattttc gacgttttct tcaatataga tcgtttgtcc
     7201 gatgatcaat ttgtaacgtt cattaagatt ttcaagtccg gtgcgcgacg aatttttgac
     7261 ctgaactttt tttcttatcg gattgttcac cacgaccgct tggtcggata cccgaatcgt
     7321 gatagccatc ggctgctgat cggaaaacgt attatgtttg atcgcattct ccaatagaat
     7381 ttgaagtgat atcggcggaa tcagcacatt atccggagaa acattatcct ccgcaatatg
     7441 caaaccgata ctttcaccaa aacgaatctt cagcagcgaa aaatattttt ctgcaaaaga
     7501 gagttcctcg ctgagtaaaa ccaaattacg attgcggtta tataaaatat aacgaaaaac
     7561 ctccgcaaga ttgtcattaa acatacgcgc tttgggcgga tcctgttcga tcaggtgcga
     7621 aagcgtatta agcgaattga acatgaaatg cggatcaatc tgatttttca acgcttctaa
     7681 ttccgcctgt gctttggcct tttcaagcgc ttcacttttg gttttatcag tttcccatga
     7741 tttgataaga aagacggttt catatgtatg cgttataaac accacgcaca ccacgcatag
     7801 cgctgtcgct ttgaaaatca cgtcccaatc cgcactgcgg tgctgcgcaa aaacatacca
     7861 agccgtcatc agtccgaccg caatcggtac ggtataaaac atattggaaa ccatcaacgc
     7921 gagcacttta tacatcggct gactgagcca tgaaaaccga atgcgtaaac gaaataacaa
     7981 ataccgatta ccatgccaaa tcgcaaacgc gataaacata aaatagagat agtgcaaaac
     8041 gagttgatta acggtatagg cgctattatc aatcatgcct gtgatattgg gaatcactat
     8101 gccgaaaaaa ggaatcataa tcacacgtat tgtgcgatca ttcaattcct gatcaatatt
     8161 ttcctcgttt agcggctcca tgatttgcca atctacatac tattcatgct ttgtgcaaaa
     8221 atttcactcc gattcagctt cgtattttcc ggtttcagtt cgcctttttt cggtttgagt
     8281 agaataacct ttgcagtgcc tccatattgc gtttaacttg cgatcgtatt taaaaaaata
     8341 ccctcatttt tttattcaaa cggaggaatg atgtatggac atggcaagtg catttaattc
     8401 actgaattgg ctggccatta tcgtagccag tatttcggcc tttgtgctcg gcggtatttg
     8461 gtattcgcca ttgatgttcg gcaatgcctg gatgaaagtg gccggtatga ccgaagaatc
     8521 cgtcaaacaa ggaaatcccg gtaaaatcta cggcggtgct tttttactga cattgatcgc
     8581 ggcgatcaat ttaggaatgt tcctcggcgc ccaaagcgat atggcgtttg gtatcgcggc
     8641 cggtgcagcg acgggtatcg gctgggttgc tcccgcgttt ggtgtggtct atctttttga
     8701 gcagcgcccg acacttcagt ggtttatcaa tggtgcctat tgggttgtca catttatcgt
     8761 gatgggtgcg atcatcggtg catggcacta acaaagttta tagatataaa aaaaccacgg
     8821 caactaaccg tggttttttt atgctattca aaatcacacg aaccgatcgg cgcgcccggt
     8881 ggtgctggtt taggcttgta aggcagtaca aaacttttcg gctcatcatt aaattctcga
     8941 atcaatcgag ctattccttc atagtgg
//
LOCUS       DAOQNQ010000118         8940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2886_length_8940_cov_4.745301, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000118 DAOQNQ010000000
VERSION     DAOQNQ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2886_length_8940_cov_4.745301"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PK754_08530"
     CDS             <1..472
                     /locus_tag="PK754_08530"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HNB09589.1"
                     /translation="AERSVLNEILKLKRQLAGFRRTVSPQRDVLSRLIHLQHPAMSKA
                     SVVYFRDIFDHLIRVNELIDTYRDTMSNVLDAYYSVLSHQINENSHIVNIIMKRLTII
                     TTIFMPLTFIAGIYGMNFHNMPEIRWEYGYYYALGLMGALALSMFYFFRKKFWF"
     gene            489..2747
                     /gene="purL"
                     /locus_tag="PK754_08535"
     CDS             489..2747
                     /gene="purL"
                     /locus_tag="PK754_08535"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HNB09590.1"
                     /translation="MHTTKAIELHEIREPEVTHKLAKDHGLTDEEYERIQQILGRKPT
                     FTELGIFSVMWSEHCSYKNSILILKTLPKQGQFVLKGAGEENAGLIDIGDGLAVAFKI
                     ESHNHPSAIEPFQGAATGVGGILRDIFTMGARPIAALNSLRFGHLDDERVRYLFANVV
                     KGIGHYGNCFGVPTVGGEVYFEECYTDNPLVNAMAVGIVRHDQTASAIAKGEGNPVMI
                     VGSATGRDGIHGATFASEEISEASEAKRPNVQIGDPFTEKLLLEATLEAIQSGHLVGI
                     QDMGAAGLTCSSSETSAKGESGIEINLELVPTREDKMTPYEIMLSESQERMLLIVKKG
                     YEKEIQNIFEKWDLHAVTIGHVTNDKLMRVKYKGRVFAEIPADTLVLGGGAPVYKRES
                     AEPAYLKETRALDIASLPQPSDLTSVLTRLLSTPNIASKEWVYEQYDSMVRTNTVTLP
                     GSDSAVVRIKGTNKALAMNVDCNGRYVYLNPRRGGHIAVAESARNVVCSGGRPAAITN
                     CLNFGNPYKPENYWQFKEAVEGMGEACRIFDTPVTGGNVSFYNESRNNAVFPTPTIGM
                     IGIIEDLKHVTTSWFKSEGDKIFLIGKNGTDIGGSEYLKTIHGKISGDAPSLDMAYEK
                     KVQDFVLDTIIKGWVRSAHDVSDGGLAVALAESCYQGKPGHTIGATIRLNDALRPDFL
                     LFSETQSRIILTTDTENAERLKQWAQTQHVDCVEIGVVGGKTLRINDWIVMQASDIER
                     LHRGAIRTTMTK"
     gene            complement(2829..4394)
                     /locus_tag="PK754_08540"
     CDS             complement(2829..4394)
                     /locus_tag="PK754_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HNB09591.1"
                     /translation="MQKKRIIWADDEIELLKPHILFLEQRGYEVTPVTNGEDAISQVR
                     TRKYDLVLLDEMMDGKDGLTTLEEIKENSPTIPVVMITKSEEERLMEDAIGRKIDFYL
                     TKPVNPSQILMACKQIFESRKLTEQKISRDYVQEFNRISQKLMDRLEFKDWYDIAQSM
                     AEWEVELDALTDTGLRQMLEDQRKACNVEFGKFVEKNYHGWVNSGLPQRPEFSVDIVR
                     NYVFPKIAARRKVVFIVIDCLRIDQWLGFESVLQEYFNINKKFYYSILPTSTPYSRNA
                     IFSGMWPSEIEQKFPDIWSKGEEDDSSRNRFEKELLEYQLKSNRVPMETEAKYVKILN
                     AEHSRQLETNITSFVNSSQLTAIVLNFLDILAHTRSDSHVIKEIAPHEPAYRSLSNSW
                     FTHSSFLEILKTLSRLDCQIVITSDHGSIRSLHGTEVIGDKETSTSLRYKYGKNIKSN
                     PKHSMFIKNPLEYKLPKRGVNTHYIIAKEDYYFLYPTNYHHFLNHYNDTFQHGGISVE
                     EVIMPVIEMEGKR"
     gene            complement(4467..6473)
                     /locus_tag="PK754_08545"
     CDS             complement(4467..6473)
                     /locus_tag="PK754_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNB09592.1"
                     /translation="MAEKKNTNRFDNLEIQPRSHSETVAHPAPGSTKNERFEWIEVPQ
                     QKTFTKRFNTKARSITSKRCPRCGWLDQSAVTECFRCGYDFQTNDMHLDFFKQEKIDL
                     PPVEIKVDMGFQDVLSKKQYDTLDAYQLRLEAERLNLISGFDRLIAVNDLNITHYDYQ
                     INTALEALRRMRGQVLLADEVGLGKTIEAGIITKELIERNLAKNILILTPASLVGQWQ
                     DELLMKFGENFIVPQQESDWRAPKVIASIDMAKRNEAANILLSRDFDLLIVDEAHRLK
                     HRSTIGFKFVNKIRKKYVLLLTATPVHNDLTELYSLVTILKPGLFGTIRSFKRKFMLK
                     DDPRLPNNEDQLKHILQDVMIRNRRDKVGINLPPRKAAIYHLDLTPIESELYREVTNY
                     VREEFRREANFDVHILSLITLQREVCSSPQATRHTLINFMNRNYPAKTRERLAYFVEL
                     CDAVKISRKSAATIELLKKFPSKTIIFVEFIETMKHLQMELEQEGISVELFHGGLSGT
                     HARKEAIDRFRKSKQVLISTQAGGEGLNLQFCSQIINYDLPWNPMRVEQRIGRVHRLG
                     QERDVFVFNLSVTDTIESRILELLANKIRMFELVIGELDIILGDIETEKNFEQQLMEI
                     FMRSTDEKEMNQQLERFGNQLTHVRRQYDKVRENSEILSDIVDV"
     gene            complement(6598..6987)
                     /locus_tag="PK754_08550"
     CDS             complement(6598..6987)
                     /locus_tag="PK754_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09593.1"
                     /translation="MKTLLMAVCFFVLQDQPAFDPSLMYGRWKWYASEGGLMEQRITV
                     EQTQQNKSLLFTKDHAVFYMVGDSVSYKSRYVTEMHRSEFTGQDTPLLRIQGISKDQW
                     ITMQGKDSLILRENKYDGWTHRYVRLH"
     gene            7076..7654
                     /locus_tag="PK754_08555"
     CDS             7076..7654
                     /locus_tag="PK754_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09594.1"
                     /translation="MTDITIYTFFPLIFTTIFWHEAGVIFWFRALESDFGHWTNAAAA
                     TLVIVSVIDFFWLKIMFFLFRTSFQRIGQTAWSHRIIARLEETKWFPRFRGFFVKKEI
                     DHQTAETPDETSGFKRFIKKTGYWGILFCGSLPGPGVKEIGIIMALTPKYRDRGFMLM
                     YIGGVIKTIMTMLVYGGLYNLIEHYLRNQIAF"
     gene            7670..8467
                     /locus_tag="PK754_08560"
     CDS             7670..8467
                     /locus_tag="PK754_08560"
                     /inference="COORDINATES: protein motif:HMM:NF023899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin ligase family protein"
                     /protein_id="HNB09595.1"
                     /translation="MEGIIIGLVCFGIAVLLLYFRKKNQDKLLEIKFVKTSTAQELKE
                     ICKQVNDELSTTGAFRQQTEVKGVIQCAHPITSELAKQPCVWYEMSVDERYEETYTER
                     DQNGREVRRTRTGTENVASNSQRVHFEVEDATGRITINPNDADIEGTQVLNRYEQNFS
                     GGRIQLGGFSMNINIGRSGRRILGYEFTESLIPLDRRVYVLGEASDASGELMVQKPED
                     KEKPFIITLKSEEELTRGTESTIRNLMIGSIVLVVIGLVAIAYGIMK"
     gene            8487..>8940
                     /locus_tag="PK754_08565"
     CDS             8487..>8940
                     /locus_tag="PK754_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013887223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNB09596.1"
                     /translation="MLSKKLQDAFNEQINREFFSEYLYLSMSAWCKSGNMDGIGNYFH
                     VQAQEEHQHAMKFFNFIHDRGGTVTLKALDAPQSKFKSLIEIFEQTLEHEQFITKSIN
                     TLMSTALKEDDHAAVSFLKWFIDEQVEEEASVSKILHKIKLIKGEGHGL"
BASE COUNT         2453 a         2067 c         1986 g         2434 t
ORIGIN      
        1 cgccgaacgt tcggtgctca acgaaatttt aaaactcaag cgacagttgg ccggctttcg
       61 ccgcaccgta tcccctcagc gggacgtact gtcacgcctt attcacctcc agcatccggc
      121 gatgtccaag gcctcggtag tctacttccg cgatattttt gatcacctta ttcgtgtcaa
      181 cgaactgatt gatacctacc gtgatacgat gagcaacgtt cttgatgcct actattccgt
      241 actctcgcat caaatcaatg aaaattcgca tatcgtcaat atcatcatga agcggcttac
      301 gatcatcaca accattttta tgccgctgac ttttattgcc ggtatttatg gcatgaattt
      361 tcataatatg cctgaaatac ggtgggaata cggttattat tacgcactcg gactgatggg
      421 cgcactggcg ctttccatgt tttatttttt cagaaaaaaa ttctggttct aacatataca
      481 ggaataccat gcacacgact aaagccatcg aacttcatga aattcgcgag cccgaagtaa
      541 cacacaaact tgccaaagat cacggcctca cggatgaaga atatgagcgc atccaacaaa
      601 ttttaggacg aaaacccacg tttaccgaac tcggtatttt tagtgtgatg tggagtgaac
      661 actgcagcta taagaattcc attctgatcc ttaaaacact acccaaacaa ggtcaatttg
      721 tactcaaagg cgcgggcgaa gaaaatgccg ggcttataga tatcggtgac ggtttagccg
      781 tggctttcaa aatcgaaagc cataatcacc cgtcggctat cgaacctttt caaggtgcgg
      841 cgaccggtgt tggcgggatt cttcgcgata tttttacgat gggtgcgcgt cctatcgccg
      901 ctctcaattc tctgcgcttc gggcatctgg atgatgagcg cgtacgctat ctttttgcca
      961 atgttgtcaa aggtatcggc cattacggta attgtttcgg cgttcctacc gtcggaggcg
     1021 aagtatattt tgaagaatgc tataccgata atccgttggt caatgcgatg gccgttggca
     1081 tcgtgcgtca cgatcagacc gccagcgcca tagccaaagg cgaaggcaac cccgtcatga
     1141 tcgtaggctc cgcaacagga cgcgacggta ttcatggcgc gaccttcgca tcagaagaaa
     1201 tatccgaagc gtccgaagcc aaacgcccga acgtgcaaat cggtgatccg tttacggaaa
     1261 aactattact tgaagcaact ctggaagcca ttcagtcagg tcatcttgtg gggattcaag
     1321 acatgggcgc cgccggtttg acctgttcca gttccgaaac cagcgctaaa ggcgaatccg
     1381 gcatcgaaat caatctcgaa ctggtaccga cccgcgaaga taaaatgacg ccgtatgaga
     1441 tcatgttatc cgaaagccag gaacgcatgc tgctgatcgt caaaaaaggg tatgaaaaag
     1501 aaattcagaa tattttcgaa aagtgggact tgcacgcggt cacgatcggt catgtgacca
     1561 acgacaaact gatgcgcgta aaatacaaag gccgtgtatt tgccgaaatt ccggcggata
     1621 cgcttgtcct cggcggcggc gcgccggtgt acaaacgcga aagcgcggaa ccggcttatc
     1681 ttaaagaaac gcgcgcgttg gatatcgcat cactacccca accttccgat cttacatcgg
     1741 ttcttacacg gctgctgagc acgcctaata ttgcaagcaa agaatgggtc tatgaacagt
     1801 atgactctat ggtacgcacc aatacggtaa cattacccgg cagcgattcg gccgtggtac
     1861 gcatcaaagg caccaacaaa gcgctcgcga tgaatgtaga ttgtaatgga cggtatgttt
     1921 acctcaatcc gcgtcgcggc ggccatatcg ctgtggccga atcggcacgt aacgtagttt
     1981 gctccggtgg acgacctgcc gctattacca attgcctcaa ttttggtaac ccgtacaaac
     2041 ctgaaaatta ctggcaattc aaagaagccg tcgaaggcat gggcgaagct tgccgcattt
     2101 ttgatacgcc cgttaccggc gggaatgtga gcttttacaa tgaaagccgg aacaatgccg
     2161 tattccctac gccgactatc ggtatgatcg gaattatcga agacctcaaa catgtcacga
     2221 catcatggtt caaatccgaa ggcgacaaga tattccttat cggaaaaaac ggaacggata
     2281 tcggcggtag cgaatacctg aaaacgattc acggtaaaat cagcggcgat gcaccatcgc
     2341 tcgatatggc gtacgaaaag aaagttcagg attttgtatt ggatacgatc atcaaaggct
     2401 gggttcgttc ggcacacgat gtatccgatg gcggacttgc cgtagcttta gccgaatctt
     2461 gttatcaagg taaacccggc cacaccatcg gcgctacaat ccgtcttaac gatgcattac
     2521 ggcctgattt cctgctgttt agcgaaacgc aatcgcgcat catattaaca accgatacgg
     2581 aaaatgcaga acgattgaaa caatgggcgc aaactcagca tgtggattgt gtggagatcg
     2641 gagtcgttgg cggtaaaact ttacggatca atgactggat cgtcatgcaa gcatcggata
     2701 tcgaacgcct gcatcggggg gcgatacgta ccactatgac caaataacta tttcgcacaa
     2761 aaaagaggaa taggtttaat tatttgcaat taaacctatt cctcaaataa taaaatatag
     2821 gttactgttt atcgctttcc ttccatctcg atcaccggca taatcacctc ttcgaccgaa
     2881 atcccgccat gttggaatgt atcattatag tgattcagaa agtgatggta attcgtcgga
     2941 tacaaaaagt aataatcttc tttggcgatg atatagtgcg tgttgacccc gcgtttgggc
     3001 agtttatact ccagcggatt tttgataaac atcgaatgtt tgggattgga cttgatgttc
     3061 tttccgtatt tatagcgcaa acttgttgac gtctctttat cgccgataac ttcagtacca
     3121 tgcaaactgc gaatactgcc gtgatcggat gtgatgacga tctgacagtc cagtctggaa
     3181 agtgttttta atatttctaa aaacgaagaa tgcgtaaacc atgaattgct cagcgagcgg
     3241 tacgccggtt cgtgcggtgc gatctctttg atcacatgcg aatcgctgcg cgtatgtgcg
     3301 aggatatcca gaaaattgag aacgatcgcc gtaagttgtg aactattgac aaaactggta
     3361 atattggtct ccaattgccg tgaatgttcg gcgttaagta ttttgacata cttagcctct
     3421 gtttccatcg gtacacgatt ggatttcaat tggtattcca aaagttcttt ttcaaaccga
     3481 ttgcgactgg agtcgtcttc ttcgccttta ctccatatat cgggaaattt ttgctcaatc
     3541 tcactgggcc acattccgct aaaaatcgca ttacgcgaat acggcgttga tgtcgggaga
     3601 atgctataat agaacttctt attgatatta aaatactcct gcaacaccga ttcaaatcct
     3661 agccactgat caatgcgcag acagtctatc acaataaata cgactttacg acgtgccgcg
     3721 attttcggaa atacgtagtt acgaacaata tccactgaaa attccggtcg ttgaggcagc
     3781 cctgagttca cccacccgtg atagtttttt tcgacaaact taccaaattc gacattacaa
     3841 gctttgcgct gatcctcaag catctgccga agcccggtat cggtcagcgc atccagctca
     3901 acctcccact cggccatgga ttgcgcgata tcataccaat ccttaaattc cagccgatcc
     3961 atcaatttct gtgaaatgcg attaaactcc tgtacataat cacgcgaaat tttttgttcc
     4021 gtcaatttgc gcgattcgaa aatctgtttg catgccatca gtatctgact ggggttgacc
     4081 ggtttggtaa gataaaaatc aatcttccgt ccgatggcat cttccatcag gcgttcttct
     4141 tcacttttgg taatcatcac gactggaata gtggggctgt tttcttttat ctcttcgagc
     4201 gtcgtcaaac catctttacc gtccatcatc tcatccagca atacaaggtc atacttccgg
     4261 gtgcgaacct gactgatcgc atcttcgccg ttagtgacgg gcgttacttc gtaaccgcgt
     4321 tgttcgagaa ataaaatgtg gggtttgagt aattcgattt cgtcatccgc ccagatgata
     4381 cgtttttttt gcatattgac ctcggtgttt gttaaaaaaa acaagagcgg taacaatgac
     4441 gaatcgggat tactcctttt gatgagttaa acgtctacaa tatcggataa gatttctgaa
     4501 ttttcacgta ctttatcata ctgacggcga acatgcgtaa gttgattacc gaaacgttct
     4561 aactgttgat tcatttcttt ttcatcggtg ctgcgcataa aaatttccat caactgttgc
     4621 tcaaaattct tttcggtttc gatatcgccg agtatgatat ccagttcgcc gatcacgagt
     4681 tcaaacatac gaattttatt agccaaaagt tccaatatac gggattcgat cgtgtcggta
     4741 acggaaaggt taaaaacaaa aacgtcgcgt tcctgaccca gacgatgaac gcgcccaatg
     4801 cgttgttcga cacgcatcgg attccatggt aagtcatagt tgatgatctg actgcaaaac
     4861 tgaagattga gcccttcccc gccggcttgc gtactgatga gcacctgttt ggatttgcga
     4921 aacctatcaa tcgcctcctt acgcgcatgc gtccccgaca gtcctccgtg aaacagctca
     4981 acagaaatac cttcctgctc tagctccatt tgcagatgtt tcatcgtctc aatgaattcc
     5041 acaaaaatta tggttttgga gggaaatttt tttagcaatt cgatggtggc cgcgcttttg
     5101 cggctgattt tgacggcatc acaaagctct acgaaatacg ccaggcgttc acgggttttc
     5161 gccgggtaat tacgattcat aaaattaatc aatgtatgcc gtgtggcttg cgggctactg
     5221 cagacctcac gctgcagcgt gatcagcgag aggatatgta cgtcaaaatt cgcctcgcgg
     5281 cgaaattcct cgcgcacata attcgtcact tcacgataga gttcactttc gatcggcgtg
     5341 agatccagat gataaatcgc ggctttacgc ggcggtagat tgatgccgac tttatcgcgg
     5401 cggttgcgga tcattacgtc ctgaaggatg tgtttaagtt gatcctcgtt attgggaagg
     5461 cgcggatcat ccttcaacat aaattttcgt ttgaaagagc gaatggtccc gaataagccc
     5521 ggtttgagta tggttacaag gctgtataat tcggttagat cgttgtgaac aggtgttgcc
     5581 gtcaacaaaa gcacatattt tttacggatt ttgttcacaa atttgaaacc gatcgtcgaa
     5641 cgatgtttga gtcggtgcgc ttcatctacg atcaataaat caaaatctct cgacagcaaa
     5701 atattggccg cttcgttacg tttggccata tcaatcgaag caatgacttt cggcgcacgc
     5761 caatcgctct cctgttgcgg tacgataaaa ttctcaccaa acttcatgag taattcgtct
     5821 tgccattgtc caaccaatga tgccggtgtt aagatcagaa tatttttagc taaattacgc
     5881 tcaatcagtt ctttggtgat gattccggct tcgatggttt taccaagccc cacttcatcg
     5941 gctaaaagaa cttgcccgcg catgcgtcgc aaggcttcaa gagcggtatt gatctggtaa
     6001 tcgtagtgtg tgatattcag atcgtttacg gcgataagac ggtcaaaacc tgaaatcagg
     6061 ttaagacgtt ccgcttcgag tcttaattga taagcatcaa gtgtatcata ttgctttttc
     6121 gacagaacat cctgaaaacc catatccact ttgatttcta ccggaggcag gtcaatcttt
     6181 tcctgcttaa aaaaatcaag atgcatgtcg ttggtttgga aatcataacc gcaacgaaaa
     6241 cattcggtca cggccgattg atccagccat ccgcagcgtg gacaccgttt ggatgtgata
     6301 ctacgggctt tagtattaaa acgtttggta aatgttttct gttgaggaac ttcgatccac
     6361 tcgaaacgtt cgtttttagt agaacccggg gcggggtgtg ctacggtttc cgaatgcgac
     6421 cgaggctgga tttccagatt atcaaaccga ttggtatttt tcttttctgc cacgctgtac
     6481 cgataataaa aaaaccagcc tttcgtttag ggctggctaa aatgcattac attattgcta
     6541 aatcatgcgg caagttacat caaaagccga gtaaaatcaa gacttggtta ggcgcatcta
     6601 atgaagacgc acataacgat gcgtccatcc gtcgtattta ttttcacgta gaatcagcga
     6661 atcttttcct tgcatcgtta tccattgatc tttggaaata ccttgtatgc gtaacagcgg
     6721 cgtgtcttga ccggtaaatt ctgagcggtg catttcggta acatagcggc ttttgtagct
     6781 tacagaatcc ccaaccatat aaaaaacggc atgatctttt gtaaagagca gcgatttgtt
     6841 ttgttgagtt tgttcgaccg taatgcgctg ttccatcagc cctccctcac tcgcgtacca
     6901 cttccagcgc ccatacatga gcgatggatc aaatgcaggc tgatcctgaa gtacaaaaaa
     6961 acacacggcc atcaataacg tcttcacaag ctcctcgttt tctgttggac atcaaactaa
     7021 attgtcgtaa tatttcaata aattcaagcg ccaatcttga tacggttcat attcattgac
     7081 tgatattacg atatatactt tttttccgct gatttttacg acgatttttt ggcacgaagc
     7141 cggggttatt ttttggtttc gtgctttaga atctgacttc ggacactgga caaacgcagc
     7201 ggcggccaca ctggtcatcg tctccgtgat tgattttttt tggctcaaaa tcatgttctt
     7261 tttatttcgt acatcatttc agcgtatcgg acaaacagcc tggtcacacc gcataatcgc
     7321 ccgtttggaa gaaactaaat ggtttccccg atttcgcggc ttttttgtaa aaaaagaaat
     7381 agaccatcaa accgctgaaa cgcctgatga aacatcaggt ttcaaacggt ttattaaaaa
     7441 gaccggttat tggggtatct tattttgcgg ttcattgccc gggcccggtg tgaaagaaat
     7501 aggaattatc atggcgctga cgcctaaata ccgcgaccgg ggatttatgc tgatgtatat
     7561 cggcggtgtg atcaaaacga taatgactat gcttgtgtac ggtgggcttt ataatttgat
     7621 cgaacattat ttgcgaaacc aaatcgcttt ttaacttggg aggcgtttta tggaaggcat
     7681 tatcatcggg ctggtttgct tcggcattgc cgttcttttg ctctatttca gaaaaaaaaa
     7741 tcaggataaa ttattagaaa tcaagtttgt caaaacttcc accgctcagg aactcaagga
     7801 aatttgcaaa caagtcaacg atgaactatc tacgacgggc gcatttcggc aacaaacgga
     7861 agtcaagggc gtgatccaat gtgctcatcc gatcacatcc gaattggcca agcaaccatg
     7921 cgtctggtac gaaatgagtg tggacgaacg ctatgaagaa acttatacgg aacgtgatca
     7981 aaacggccgc gaagtgcgtc gcacacgaac cgggactgaa aatgtggcct ccaatagcca
     8041 acgtgtgcat tttgaagtgg aagacgcgac cggtcgcatc actatcaatc ccaacgacgc
     8101 cgacatcgaa ggcacgcagg ttctcaaccg ttatgaacag aatttttccg gagggcgtat
     8161 ccaacttggc gggttttcga tgaatatcaa tataggccga agtggacgac gcattttagg
     8221 ctacgaattt accgaatcat tgataccact ggatcgccgc gtgtatgtcc tcggtgaggc
     8281 atccgatgcc tcgggcgaac tcatggttca aaaaccggaa gacaaagaaa aaccctttat
     8341 tatcaccctc aaatccgaag aagagctgac acgcggtacc gaatctacca tacgcaatct
     8401 gatgatcggc agtatcgtcc tcgtcgtcat cgggctagtc gccatagcat atggcatcat
     8461 gaaataaata tagaaaggcc ttacttatgc taagtaaaaa attacaagac gcattcaacg
     8521 aacagatcaa tcgcgaattt ttttcagaat atctctacct ctccatgtcg gcctggtgca
     8581 aatcaggaaa tatggatggc atcggaaatt attttcatgt gcaggcacaa gaagagcatc
     8641 agcacgctat gaaatttttt aactttattc atgatcgtgg cggcaccgta actctcaagg
     8701 cattagacgc gccccaatca aaattcaaat cccttatcga aatttttgaa cagacactgg
     8761 agcatgaaca atttatcacc aaaagcatca atacgctcat gagtacggcc ctcaaagaag
     8821 acgatcatgc ggcggtgagc tttctgaagt ggtttattga cgagcaagtc gaagaggaag
     8881 cttcggtatc taaaatcctg cacaaaatca aactaatcaa aggcgaaggt cacgggttac
//
LOCUS       DAOQNQ010000119         8860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2922_length_8860_cov_6.214083, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000119 DAOQNQ010000000
VERSION     DAOQNQ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8860
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2922_length_8860_cov_6.214083"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PK754_08570"
     CDS             <1..257
                     /locus_tag="PK754_08570"
                     /inference="COORDINATES: protein motif:HMM:NF015945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HNB09597.1"
                     /translation="NTLIARHQAAKAKKMIAQQMSGIGGGAFGNFDKFEKKVEDVEAE
                     ADAHVELSGQNTSLEDEFKKLSTDTTVDSELAALKAQLGK"
     gene            complement(332..754)
                     /locus_tag="PK754_08575"
     CDS             complement(332..754)
                     /locus_tag="PK754_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNB09598.1"
                     /translation="MSNGTLSSQFEMKYMGNAVLPYPLKLAWMPEKTIKRMYCHRDMI
                     PVFQKVFNDIYQAGLAPKAELFGGCFNFRLKRTTNKLSTHSWGIAIDLNPATNALGTK
                     GNMNPSIVKIFKNNGFIWGGDWADLNCDPMHFQFCDGY"
     gene            complement(1099..2568)
                     /locus_tag="PK754_08580"
     CDS             complement(1099..2568)
                     /locus_tag="PK754_08580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB09599.1"
                     /translation="MISFLEKITGFHESELASFFDSNPNARNEFHRIANDQNRKNAVV
                     FIWFLILTQFIVGIVRIISGWGGVPYYNLALIGLGALLLFLFRYRSFRQNVTHISLIL
                     FTVWAITFPFFIIRLGGYEAPTYPVYIVIAVYILAFFNYTFRQYLYLYVIIFLSNACI
                     YFLETDVETDSYVYRQSVMLFLYMVALSGAYIHVHLRKKSFQVQYELGQRKSELEKAY
                     KQLEEKELQLVQKEKLASLGTMTAGIAHEINNPLTFIDGNLQSLAEYINDLKTLVSVW
                     DKNFDTVDQQKQYVNSIKAQIDYQFLINDLDKLLASCNNGSKRIVDIVNKLKNFSNLD
                     SAEFKTVNLCDSLETMIYLLKSQYTDVKFNFSKKCDQTESAELACNPGLLNQAIMGII
                     ENGIDAARLNKSETPKIIISLFANPEEININITDNGLGISKEVQEKMFDPFFTTKDVG
                     KGSGLGLSVAFGIIKQINGRLEFKTTQGRGTEFIIKIKK"
     gene            2724..3872
                     /locus_tag="PK754_08585"
     CDS             2724..3872
                     /locus_tag="PK754_08585"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB09600.1"
                     /translation="MEQVGINSNVYAGGKIFHIQTAGSYNDLVVQSEIFEAGRIISVQ
                     KKRITDVTEMPEDDFREFVKKYHHEVAWEVELMFFMREKIRNTTHTVSNSKIGLLFLR
                     KGFLDEAISEFQHTIEHDPDLVEVYNHLGLAYLHRGDFSLAIESIQKGIELRPQFPDL
                     RNNLGFAYGKAKKYSEAITEYDKALAINPHYVVVHLNKAITFLESILNNGDTGSLPTV
                     QDRILTVSSIFKTTLMTNPDFANYRFYTFRFEKILKHLTASAYVQAIDIILEVKESIQ
                     KPSLDETIHGFYLKFLFGGAGKDEKVINDYRAQLEQHINENPTYADLHNSLGLVYLIQ
                     CRNLFLKAMSQFKKAYEINPSYKTAYKNFRLVQNDGREFLNLLRAILK"
     gene            4172..4603
                     /locus_tag="PK754_08590"
     CDS             4172..4603
                     /locus_tag="PK754_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB09601.1"
                     /translation="MKEGYLMIPSVKLNREVFDNMKLCNRKTVLVVEDEPGIRFVTSE
                     FLKMSNFQVVTAENGQEAMKIIVDNKIDVVVTDIMMPKVNGLELLKFVRRLKGESFPV
                     VVVSGSDLESLHVIESEHTRTLRKPYGLDRLAKEILSISQN"
     gene            complement(4609..5169)
                     /gene="pyrR"
                     /locus_tag="PK754_08595"
     CDS             complement(4609..5169)
                     /gene="pyrR"
                     /locus_tag="PK754_08595"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNB09602.1"
                     /translation="METEMNEAEKSIIIARAQEINTMLEDMTRSLISSNPSLESLALI
                     GMRTRGEFIARRLQILVKKFSDIELPMGVLDVSFYRDDTRAKLKQPLVQSTEIRFNIN
                     ERPLVLVDDVLFTGRSVRAAMDELMDFGRPSRIQLAVLIDRGCRELPIQSDIYGKKIT
                     SEICDKVKVCLEEYDKIDEITLIKGS"
     gene            complement(5169..7088)
                     /locus_tag="PK754_08600"
     CDS             complement(5169..7088)
                     /locus_tag="PK754_08600"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNB09603.1"
                     /translation="MIVACLVIAFGRPVWKWTATNGNINAHAKTSVVLILDNSLSASS
                     VNQRGQVLERIKNKAMEVISHLQEGDEFVIIDASGLKKASNLNWSSNFSEARARLKNI
                     VIENYSFAIDKALHESVSLLAQAKNPNREIYIISDLQKSDRFNTQIPDYGHIKPHLVV
                     IAIDGKPESDLAILKAETENRFFQKSHPIEMVCTIRNSLSTQSGERILHVYANGKRVG
                     QSVLQVDAKTDTDVRISFPASESGFVSGYAELDDDGFLANNRYYFHYYVPSDIKVLVA
                     GELADKEFIELALKAHDQQAGNIITKSVLFDKLATVDLKKFDVLILVNPGRWDESQTN
                     RVVEFLKEGGGILMIPGTKTDITQFNNQIADRLDVSRLIQMVSLNSENHSFIELQFDA
                     QPHPLIADIFTDRTSLKNIESPKFYRYFNTDSRHGQNILTFGKSGNFLTEYAIHNGYF
                     MLMTSMPVMTWNDLPIRAFFVPLMHRCLYALYGKENDKNQFVVGDAVRVRIQRDANDM
                     VRMRNLTGEQSDYIPSLVVQNNIKIAQIDKLDMAGNYSLVDKNNSRVLTLVSANIPSQ
                     ENEFAIWESDSLKIFSGTMNVTFLTENESIENYILQSRFGIESWKWFIAVAIILMFFE
                     LALGQMHRLQRNTNK"
     gene            complement(7282..7998)
                     /locus_tag="PK754_08605"
     CDS             complement(7282..7998)
                     /locus_tag="PK754_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB09604.1"
                     /translation="MIASAESKKILVRAQAISKNYTMAKGELTVLKNISIDIHEGEFI
                     VILGPSGAGKSTLLHILGILDKPSSGDVFFENTQVSQLSDVAMARLRNARVGFVFQFH
                     YLLPEFTALENVIMPALIAGKPSNDPSSYERGKNLLKNVGLAERYYHRPSELSGGEQQ
                     RVAFARALMNDPGLILADEPSGNLDKRASEHLHQLMFDLCREFNKTFVVVTHNEALAE
                     RAQRVIRIEDGGIDSDIRKA"
     gene            complement(7995..8831)
                     /locus_tag="PK754_08610"
     CDS             complement(7995..8831)
                     /locus_tag="PK754_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09605.1"
                     /translation="MRFLKFYLILFLTVASAAFSQKDRLVVLTYKFNEGDKYRVAVST
                     YKNFSTISSAYNNSVYGETAYDIHQNILHVDGEGSAQIANDIELTRYTINGQNLTYKM
                     AKLFANDHVEIGVDRFGTLLEKSLTLKNGVLAESGIENIWRYMFIPFPEKTLKIGETW
                     TLNDAAGDSAIILPFKKLLKITDPTLTVRYKLEEADKSTAKIKLSLEVNGEGNIPDHK
                     EQTKINFLIILEGTMVYSFSEGRILNGSLVTQLAGTGTVGSKAVEYSGSLNTTFTIEE
                     AQ"
BASE COUNT         2826 a         1700 c         1526 g         2808 t
ORIGIN      
        1 aaaatacatt gatagctcgc caccaagctg caaaagctaa gaaaatgatt gctcaacaaa
       61 tgtccggtat aggcggcgga gcttttggca actttgataa gtttgaaaaa aaggttgaag
      121 atgttgaagc tgaagcggat gcccatgttg aactttcagg acaaaataca agtctagaag
      181 atgaatttaa aaagctatca acagacacaa ccgtcgattc ggaattagcc gctttgaaag
      241 ctcaattagg aaaataaata atatagtttg tagaattaaa tttaaaggcg aactatgttg
      301 attagttcgc cttttattat tattctaaaa attagtatcc atcacaaaat tggaaatgca
      361 ttggatcaca gtttagatca gcccaatcac caccccaaat aaaaccattg tttttaaata
      421 ttttaactat tgatggattc atatttcctt tagtaccaag tgcattagta gccgggttga
      481 ggtctatggc aattccccaa ctatgagtag ataatttgtt tgttgttctt tttaatcgaa
      541 aattaaaaca accgccaaaa agctctgctt tcggagcaag tccggcttga tagatatcat
      601 taaatacttt ttgaaaaaca gggatcatat ctctgtggca atacattctt tttattgttt
      661 tttcaggcat ccaagccaat ttaagaggat agggtaatac ggcattcccc atatacttca
      721 tttcaaattg cgatgaaagt gtcccattac tcaagatata ctttcgtata tcaccgaatt
      781 gctttacgat ctcggcatac ccattaggcg tgttcataat tattgccatt catatattta
      841 acaattcaaa ataaccaacc actaaacggt tatcactaat tgtaaagatt ttaacattgt
      901 tttatgtagt agcaataaga attacttgga tattaaatta gagcttttta ttgcgacata
      961 cgaattcgag tatttctaaa atacacataa tttgtcttag aatccagcaa taaattaact
     1021 tttaattgaa agatatatat accaccatag cacaacttag atatataata tatattatgt
     1081 aaacttatta aaatccactt acttctttat ttttattata aattcagtcc ctcttccttg
     1141 agttgtttta aactcaagac gtccattgat ctgctttata atcccaaaag ctacgctgag
     1201 cccgagtcca ctacctttgc cgacatcttt ggtagtaaag aatggatcaa acattttttc
     1261 ttgtacttct tttgatatgc ctaagccgtt atctgtaatg tttatgttaa tttcttctgg
     1321 attagcgaac aaacttatta ttatttttgg ggtttctgat ttgtttaaac gagccgcgtc
     1381 aataccattt tcaataatac ccatgatagc ttgatttagt aatcctggat tgcaagcaag
     1441 ttcagccgat tcagtttgat cgcatttttt tgaaaaatta aatttaacat ccgtatattg
     1501 gctttttaat aaataaatca ttgtttcaag actatcacac aaattgactg ttttgaattc
     1561 ggcagaatct aaattgctaa aatttttaag tttattaaca atgtcaacaa ttcgtttaga
     1621 accattattg catgaagcca gtaatttatc taagtcattg atcaaaaatt gatagtcaat
     1681 ctgtgcttta attgaattaa cgtattgttt ttgttgatct acggtgtcaa aattcttatc
     1741 ccaaacagat actaatgttt ttaaatcatt gatatattcc gctaatgatt gcaggttacc
     1801 atcaataaaa gtcaaaggat tgtttatttc atgagcgata cccgccgtca tagttccaag
     1861 tgatgccaat ttttctttct gtacaagttg caattctttc tcttctaatt gtttataagc
     1921 tttttctaat tcagatttac gttgacctaa ttcatattgc acttgaaatg actttttcct
     1981 caaatgcaca tgtatataag cgccggacaa agcaaccata tacaagaata acatcaccga
     2041 ctgacgatag acatagctat cggtttcgac atctgtttca agaaaataaa tacacgcatt
     2101 ggacaaaaat ataatcacat ataaatacag atattgtctg aaggtgtaat taaaaaaagc
     2161 taaaatatac acggctatta cgatgtaaac cggatacgtt ggcgcttcat agcctcccaa
     2221 acgtatgata aaaaaaggaa acgtaatggc ccaaaccgtg aaaagaatca gagatatgtg
     2281 ggtaacattt tgcctgaatg aacggtaacg aaataaaaac aatagaagcg cacccaaacc
     2341 aatcaaagct aaattataat acggaacgcc tccccatccg cttataatcc gtacaatgcc
     2401 gacaatgaac tgagttaaaa tcaaaaacca tataaatacg actgcatttt tcctattttg
     2461 atcattggct attcgatgaa attcgttacg tgcattcgga ttcgaatcaa aaaaacttgc
     2521 aagctccgat tcatggaagc ccgtaatttt ttctaaaaat gaaatcatag ttatgttata
     2581 tgagatgtgt aaattggctg aactaataaa tgataatcat ttaatgtcca gttatcaaaa
     2641 tatttcttgt atattcagca ttctgatgtt aatttaatcg ccgtaggtcg gtttctcaca
     2701 ctaaaataaa aatatctttt ttcatggaac aagtaggcat taatagtaat gtatatgccg
     2761 gtggtaagat tttccatatc caaaccgctg gaagctataa tgatctcgtt gtacaatctg
     2821 aaatttttga agccggtcgc attatttctg ttcaaaagaa acgtattact gatgttacag
     2881 aaatgccaga agatgatttt cgagaattcg tcaaaaagta tcaccatgaa gtagcatggg
     2941 aagttgagct catgtttttt atgcgtgaaa aaatcagaaa caccacgcat acggtatcca
     3001 atagcaaaat tggcctttta ttcttgagaa aaggctttct cgatgaagcc atttccgaat
     3061 ttcaacatac gatcgagcat gatccggatc ttgtggaggt ttacaatcat ttaggactcg
     3121 cctatctcca tcgcggcgat ttttcgttag ctatcgaatc tattcaaaaa ggtatcgaac
     3181 tgagacctca atttcctgat ctgcgcaata acctcgggtt tgcatatggt aaagcgaaaa
     3241 agtatagtga agcgataacg gaatatgata aagcattagc gattaacccc cactatgtag
     3301 tcgtgcatct aaataaagcg ataactttcc tcgaaagtat tctcaacaat ggcgatacgg
     3361 gaagtcttcc gaccgttcaa gatcgaatac tcaccgtaag ctctattttt aaaaccacat
     3421 tgatgacaaa tcctgatttt gcaaattatc gtttttatac atttagattt gaaaagattt
     3481 taaaacactt gaccgcatca gcttatgtcc aagccattga cattattttg gaagtcaaag
     3541 aatctattca aaaaccaagc cttgatgaga ctattcacgg attttattta aagttccttt
     3601 tcggaggagc cggtaaggat gaaaaagtta ttaacgacta ccgcgcacaa ttagaacaac
     3661 atataaacga aaatcctacg tacgccgatt tgcataattc acttggttta gtatatctga
     3721 ttcaatgcag aaatttattc cttaaggcca tgtcgcaatt caaaaaagct tacgaaataa
     3781 acccatctta taaaaccgca tataaaaact tccgcttagt gcagaatgac gggcgcgagt
     3841 tcctcaatct acttcgtgct atcttaaagt aattccaacc tttatcatct ttttctcaat
     3901 ttcttttatt tctcattcca tgactgtttt atcatttcac agtataatct aataatggta
     3961 gtctcttcct caaaaaacat caatatttgt atttttttga gaaaatgaat tgccatcgaa
     4021 caagtataat aattttgagt gttaagccca tcacagaaac actgtaaact gcatcacaga
     4081 aaacaattta aactctataa aaacatacaa ttgaattttt aatgataaca ttggtacgtt
     4141 ttttgatcta attgattatc agttctaaat aatgaaagaa ggttatctga tgatccccag
     4201 tgtcaagcta aatagagaag ttttcgataa catgaaactt tgtaaccgaa aaacggtgct
     4261 tgtggtagaa gatgaacccg gaattcgttt tgtaacgtca gaattcctaa agatgagtaa
     4321 ttttcaggtc gtaactgcgg aaaacggtca agaggctatg aaaattatag tggataataa
     4381 aatagatgtc gtggtaacgg atatcatgat gcctaaagta aacgggttgg aacttttgaa
     4441 atttgttcgc cgactcaaag gtgagtcatt ccctgtcgtt gttgtatccg gttcggattt
     4501 agaaagtttg cacgtcattg aaagtgaaca tacacgcacg ctgagaaagc cttacggatt
     4561 agatcgtttg gcaaaagaaa tactaagtat atctcaaaac taaactcatt aactaccttt
     4621 aatcaaagtt atttcatcaa ttttatcgta ttcttccaaa caaactttta ctttatcaca
     4681 tatttctgaa gttatttttt ttccataaat atccgattgg atcggtaact cgcgacatcc
     4741 cctatctata agtacggcga gctgaattcg tgatggtcgc ccgaaatcca taagttcatc
     4801 cattgccgca cgcaccgatc gacctgtaaa aagcacatca tcgactagca ccagcgggcg
     4861 ctcgttgatg ttaaaccgta tctcggtact ctgtactaat ggttgtttta gcttcgctct
     4921 tgtatcatca cgataaaagc tcacatccaa tactcccatg ggcagttcaa tatcggaaaa
     4981 ttttttaacc aaaatttgta aacgacgcgc aatgaattct ccgcgggtcc tcatccctat
     5041 taaagctaat gactcaagac ttggattgga agaaatgagc gatcgcgtca tatcttcaag
     5101 catggtatta atttcctgcg cccttgctat gattatactt ttttctgctt cgttcatttc
     5161 tgtttccatt atttattggt attacgttgc aaacgatgca tttgaccaag tgctaattcg
     5221 aaaaacataa gaattatcgc gacggcaata aaccatttcc aactttcgat accaaatcga
     5281 gattgtaaaa tatagttttc aatagattca ttttcagtta aaaaagtaac attcattgtg
     5341 ccgctaaata ttttaagaga gtccgattcc catatagcaa attcattttc ttggcttgga
     5401 atattggcgc ttaccaacgt aagcactcgg gaattgtttt tatccacaag agaataatta
     5461 cccgccatat caagtttatc aatttgagct attttaatgt tattttggac tactaaagac
     5521 gggatgtagt cgctctgttc gccagtaaga ttgcgcatac gaaccatatc attggcgtcc
     5581 ctttgaatac gaactctgac agcgtcaccg acaacgaatt gatttttatc attttcttta
     5641 ccatacagag catataaaca acgatgcata agcggtacga aaaacgcacg aattggaaga
     5701 tcattccaag tcataacggg cattgatgtc atgagcataa aataaccatt gtgaatggca
     5761 tattcggtta aaaaattacc gctttttcca aacgttagga tgttttgacc atggcgtgag
     5821 tctgtattaa agtaacgata aaattttggg gactcaatat tcttcaaaga agtgcggtcc
     5881 gtaaaaatat ctgcaatcaa aggatgcggc tgagcatcaa attgaagttc aataaacgaa
     5941 tggttttcgc tattgagcga aaccatctga atcaaacgcg atacatctag cctatccgcg
     6001 atttgattat taaattgggt gatatccgtt ttagtgcccg gaatcatgag aattccacca
     6061 ccctctttca agaattctac gactcggtta gtctgagatt cgtcccaacg gccagggttt
     6121 acaagaatca gaacatcaaa ttttttcagg tcaacggttg caagtttgtc aaacaaaact
     6181 gatttggtaa tgatgttacc tgcttgttgg tcatgcgctt taagcgcaag ctctataaat
     6241 tctttgtctg cgagttcacc ggccacaaga actttgatat ctgatggaac ataataatga
     6301 aaataatatc tattatttgc taaaaaacca tcatcatcta actccgcgta accggaaaca
     6361 aagcctgatt cggatgctgg aaatgaaatg cgcacatcgg tatcagtttt agcatcaacc
     6421 tgtaaaacgg actggcctac tcgtttccca ttagcataga cgtgcaaaat cctttcaccg
     6481 gactgggttg acaatgaatt tcgtatcgta cacaccattt cgatcgggtg acttttttga
     6541 aaaaatcgat tttccgtctc agcttttaat atggctaggt ccgattccgg ttttccatca
     6601 atagcgatga caactaaatg tggcttgata tggccgtaat ctggaatttg agtattaaat
     6661 cggtcggatt tttgcaaatc agagatgata taaatttcac ggttggggtt tttagcctgt
     6721 gctaaaaggc tcacggattc gtgcagagct ttatctatag caaatgaata attttcgata
     6781 acaatatttt taagtcgagc ccgtgcttca gaaaaattag aactccaatt taaattagaa
     6841 gcttttttta atcctgacgc atctataata acaaattcat cgccttcttg aagatgcgaa
     6901 atgacttcca ttgccttatt ttttatgcgt tccagcactt gtccgcgttg atttacggac
     6961 gaagcgctga gtgaattatc aagtataagt acaacagaag ttttagcatg ggcgtttatg
     7021 tttccgtttg ttgcggtcca cttccacacc ggtcgcccaa atgctataac gaggcacgct
     7081 acgatcatcg ctctgataat taacaataat aactgttgcc aacgtaactt tttaatttgt
     7141 tttttttgga gtgcttttaa aaaacgattg gagctaaaag gaacaacacg tgcttttttt
     7201 cgattgagca gatgaatcaa tattggaata gatgccaata ccagaccata caataaaaaa
     7261 gcatttaaga aactaaacat atcatgcttt ccgtatatcg gaatctattc cgccgtcttc
     7321 gatacgaatc actcgttgtg cacgttcagc caaagcttcg ttatgtgtta ccacgacgaa
     7381 tgttttatta aattcgcggc aaagatcaaa catgagttga tgcaggtgct cgctagcgcg
     7441 cttatctaaa tttccggaag gttcatcggc cagaataagg cccggatcat tcatcaaagc
     7501 gcgtgcaaac gcaacgcgtt gctgttctcc gccggatagc tcagagggtc tatgataata
     7561 acgctcggca agacccacat ttttcaaaag atttttgccc ctttcataac ttgacggatc
     7621 gttcgaaggt tttccggcaa tcaatgcggg cataattacg ttttccagcg ccgtaaactc
     7681 cggcagcaaa taatggaact gaaaaacaaa accaacacgc gcattacgca aacgtgccat
     7741 agcaacgtct gataactgac tcacctgtgt gttttcaaag aaaacatcac cggaagaagg
     7801 cttatctaat atacctaaaa tgtgcaatag tgtacttttt cctgcgccgg atggtcctaa
     7861 aattacaata aattcgccct catgtatatc tatactaatg ttttttaaaa cagttagctc
     7921 acctttggcc atggtataat ttttactaat ggcttgtgcc cgaacgagaa tttttttgga
     7981 ttcggccgaa gctatcattg cgcctcttcg atagtaaaag ttgtattaag cgatcccgaa
     8041 tattcgaccg ccttcgaacc gacggtacct gttccggcaa gctgagtaac caaagacccg
     8101 ttaagaattc gcccctccga aaaactgtac accatcgtgc cttcgagaat aattaaaaaa
     8161 ttaatttttg tctgttcttt atgatcagga atatttccct ctccattaac ttccaatgat
     8221 agtttgatct tagccgttga cttatctgct tcttccagct tataccttac agtaagcgtc
     8281 gggtcagtta ttttgagtaa ttttttaaac ggtaaaatga tcgctgaatc acctgctgcg
     8341 tcgtttaatg tccatgtctc tccgattttg agagtttttt caggaaatgg tataaacata
     8401 taacgccaaa tgttttcaat accgctttca gctaaaactc cgttttttaa ggtcaacgat
     8461 ttttctaaca atgtcccaaa acgatcaaca ccgatttcaa catgatcatt ggcaaaaagt
     8521 ttagccattt tatatgtaag attttgtcca ttgatcgtgt atcgtgttaa ttcgatgtca
     8581 ttagctatct gcgcactgcc ttcaccgtca acgtgcaaga tattttgatg aatatcatag
     8641 gcggtttcac catatactga attattgtat gcggacgaaa tcgttgaaaa atttttatac
     8701 gtactgacag caacgcggta tttatcgcct tcattaaatt tgtaagttaa tacaacaagc
     8761 ctgtcttttt gtgaaaaagc tgcactcgca acggttaaaa acaaaattaa atagaatttt
     8821 aaaaatcgca taaaatcctt atgattttaa agcttcgagt
//
LOCUS       DAOQNQ010000120         8815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2945_length_8815_cov_6.338356, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000120 DAOQNQ010000000
VERSION     DAOQNQ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2945_length_8815_cov_6.338356"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1749
                     /locus_tag="PK754_08615"
     CDS             <1..1749
                     /locus_tag="PK754_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09606.1"
                     /translation="ADHVFSKFEIHHSRAEESLTTVRTGKLLANADVYESAMREIKQD
                     FEEAKKTFNEKSRFISVQSKIRRMVSDVQDQVASLKQRSNYDSYMTSQAKGDLDEART
                     ALQNAIKEWPQLSTSSYAADLLQYEKELQAIESSGNASEGFKKLIDKAWFEGKFLLQN
                     GLQYGSFTKDAAQSFYENCAKINYPKALSALNSAKPNNDVNSSQIEYLTKEFPTEFNA
                     YLKKGYIADINSHIEKAYAYKANGKTQLSLALEEAETAVIMADAVLLVKPDDVSIQRL
                     RKDGLSTVEKMKTEIDAAEATAFTSVFHKENKSKIVFSKNRISLKQENASTSSDFFNG
                     GEPIYAMAYFTAMVKDLPKMYRTANPVVRIKYYVDVPADDAYAVPTATTEYQIMASNK
                     TLMEATTLEVEVAPNAGQTDRPEVGAQAAKMLSQLSPRLHSIRVVLSIDNENFATGSF
                     ELDGSQGLEKFAALGDDLIKSKIQNARPPKGGGLEKTIEQGIRSALKSEGFNVLRVIQ
                     TDPQWYYERHPISGAILNRTIQAAIIYKDEQNKCIVEYMEFTQKYINSQYDALYRSMR
                     GTVTKPYEVLPEHVNE"
     gene            1810..2886
                     /locus_tag="PK754_08620"
     CDS             1810..2886
                     /locus_tag="PK754_08620"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09607.1"
                     /translation="MIKILLSKWPRIHWHVFLLGFAILSCDSKETNEDKVEPVNPYTD
                     LRWRVSLTKVAKAITSNPVNPNTIYVNTYSDYVGRGGIFISYDRGETWPAAMSPTIDT
                     STNQGAGDVQFIAVCPNDTHIVFAGGVHSSGLLRTSNGGKNWVNVLPGVDADAEAMAF
                     NPQNPAQVFFMSYTDAGFYVSQDTGRTWQLRKFTGFGYSCTLRMHPSDPNIFVAGASK
                     GKIIKTKDQGYSWYLVQKQVSNQPNEWPEVPAIIWDNENPNKLYATISGANGKSFSQS
                     NNGGETWHIKDLKEELWAAHMTKSKLVIFGGWQSTYITSDQGKNVFKIGGDETVSGYW
                     SITSSGNRILAISSYDRSLMMVDL"
     gene            2932..3393
                     /locus_tag="PK754_08625"
     CDS             2932..3393
                     /locus_tag="PK754_08625"
                     /inference="COORDINATES: protein motif:HMM:NF027592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HNB09608.1"
                     /translation="MKLLIRGILIMLLVSGCSKNEQQENADKFGEGITLKKVVRMPEV
                     LNNPETYDGQEIVVESTIKTVCQARGCWMEVESGILPMRISFKNDSYFIPKDAARRKV
                     KMQGVLKVKEVSEEVARNYLKEAGRSDEEIGKINGAQRLLTMVASGLAIYK"
     gene            3482..4234
                     /locus_tag="PK754_08630"
     CDS             3482..4234
                     /locus_tag="PK754_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09609.1"
                     /translation="MKYILVIALLVSLGRQSLAQTETIEHGVGFYVKGGWFFDRSSGS
                     EDHSPELKGLKGELGNDISDRSVYIGVRYKKIEAEMGWGEIIKNSDITIYPVESRAAR
                     YWAGSVAYYVPFQIGSSNPIKSKLALGVKYSTYNTSYVDESITKLSIDKWNIKSTWFP
                     ERSYFSFLFPTVSFFYENQQKLANQSEFGPAHSIGIALKPAAVFPLGILPVKIVIEPG
                     MSWSHKLSGSDHFQKVYDTVYSLDAAIVYNLK"
     gene            4276..4680
                     /locus_tag="PK754_08635"
     CDS             4276..4680
                     /locus_tag="PK754_08635"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HNB09610.1"
                     /translation="MGELNFLAIAVAGFAYFMLGALWFSPFVAGTYYDKALGFTRTKD
                     MKWGVIYYIGPFMGCVISTFATAVLMRWCSVISMGEATKLGLIVGMGYGMVISITNAI
                     NPNTPRPLMYGLATGGYHVIGNIMVSVLIYIM"
     gene            4861..5649
                     /locus_tag="PK754_08640"
     CDS             4861..5649
                     /locus_tag="PK754_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB09611.1"
                     /translation="MSADYNRFSEKYGDHLSQEGDLYIPIGQPKAIVCFFHGGFWRAP
                     YGRDQFDSVAIDFAQAGYIIWNIEYRRVGEVGGGWPGTFEDAILSVNYLSTLQKKYRW
                     SDNLNVVLAGHSAGGQLALWLTAHAEKKFLQHTPLTIHPDLCIGLAPVVNFEKAYQEN
                     LGGQAIYKLLGCTPKENPERYTCYDPLFAVHLNTPQIILHGEMDEDLPIAYSISYLRA
                     AEARNTSIDLIELEKTGHMDFLEPQSLAVKRWKMLLYLRYGIHK"
     gene            5727..6290
                     /locus_tag="PK754_08645"
     CDS             5727..6290
                     /locus_tag="PK754_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB09612.1"
                     /translation="MSLLRIIAKPLPDEYPMYSAKYIDLLKNDGLVLYHLMNNFLEIR
                     HNITELSSEILHYRYAPEKWNIKETLVHMMDDERIYAYRALCFARGEKQKLPGFDQDS
                     YTALSGASARSIQSIFVEYEAVRHATVAMFNAFTDEMLLRMGYSDENNSHRSVRAFVY
                     HIAGHELRHWEIIKERYLDRSASCGTV"
     gene            6318..7016
                     /locus_tag="PK754_08650"
     CDS             6318..7016
                     /locus_tag="PK754_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09613.1"
                     /translation="METETFKIDIQNILGKDFAEHELIHSFYGETFALEDQFFPPVVS
                     YALPRILQCESAGVLACSLPDEHAEQMLHYWFGSKDSAYISSGSIRVASHCNSCPDSC
                     TGICPVLMREDPQYFLPPYHVRGYPFSGSIRGHIVLSDPKPLRIHPEGERLIIRTTKR
                     LFNQFKMQFHVDLGADHQAVDALVPICSFCKKIRDDEYQWVRLEKFFNDNYSIQFTHS
                     ICPHCSHEYIKTIK"
     gene            7124..8542
                     /locus_tag="PK754_08655"
     CDS             7124..8542
                     /locus_tag="PK754_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09614.1"
                     /translation="MKSMFLSYFVCFAFVSHTFVVSQIKPSDRVISKVVCQYDTSQSY
                     VLFLPSGYDSSRKYPILYCFKPEKDALYPVVYYADIMERYGVIMVCSWNSQNGPWQVC
                     IDALHAVWKDTHHRFSIDLGRVYATGFSGGARTASELAIRYPHQVTGVIGIGSGFSTL
                     QAIPQAVPFRYFYGLYGNRDYNKSEMIYLDEELSKRQMMHRIVEFEGIHQWAPFTDFS
                     DGFEWMMLNEMRDGKRTPDSAWIAQLFDKRHEQLKTSLHQKNFVDAARMARAIYDDFQ
                     FTMPCSAFVALADSVQKLKAYSDEMALRSDLQQREVHTQNIMDDFLFNLENPLFEPER
                     DMQLQMLYKLTKEWQPLYNTKNEIKSNAAYRCLDYIARLCRARGNGYLNQRVLPKAVA
                     CFDLARTVWPDDASSHYNLACALALSGKTNKALDALETAVKVGFNDVPLLDTDPDLNS
                     VRSDKKFNSIRKKAEHSTTSKK"
     gene            8619..>8815
                     /locus_tag="PK754_08660"
     CDS             8619..>8815
                     /locus_tag="PK754_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09615.1"
                     /translation="MKWFYVLLFVFVVVFSYCLLQAKQAVMDTSSIEQAVLNYHTYAE
                     WLTYGVPAAFLILLILANFSFT"
BASE COUNT         2613 a         1625 c         1958 g         2619 t
ORIGIN      
        1 gccgatcatg tattttcgaa gttcgagata catcattctc gtgcggagga atcattaaca
       61 acagtacgca caggaaaatt gcttgccaat gccgatgtct atgaatcggc tatgcgcgaa
      121 atcaaacaag attttgaaga agcgaaaaaa acattcaacg aaaaatcgcg attcatttca
      181 gttcagtcca aaattcggcg tatggtatcc gatgtacaag atcaggtcgc gtcattgaaa
      241 cagcgctcca attatgatag ctacatgact tcgcaagcga aaggcgattt agatgaagcg
      301 cgcacggcac tgcaaaatgc cattaaagaa tggccgcaac ttagtacatc aagttatgct
      361 gcggatttat tgcaatatga aaaagaatta caggctattg aatcgtccgg gaatgcgagt
      421 gaggggttta aaaagctcat agataaagcc tggttcgagg gcaaattttt gttgcaaaac
      481 ggattgcaat atgggtcatt cacgaaagat gcagcacaaa gtttttatga aaactgtgcc
      541 aaaataaatt accccaaagc actcagtgca ttaaatagcg cgaaaccaaa caacgatgtc
      601 aattcgtcac aaatcgaata tttaacaaaa gaatttccga ctgaatttaa tgcgtatctc
      661 aaaaaagggt atatcgctga tatcaattcg catattgaaa aagcctacgc atataaagcc
      721 aatggtaaaa cacagttgag tctggcctta gaagaagccg aaacggccgt aattatggcc
      781 gatgccgtgc ttttagtaaa acccgatgat gtttcgatcc aacgtttgcg taaggatggg
      841 ttatcaactg tcgaaaaaat gaaaacagaa attgatgccg ctgaagcgac agcttttaca
      901 agcgtctttc ataaagaaaa caaatctaaa attgtttttt ctaaaaatag aatttctctc
      961 aaacaggaaa acgcgtcaac ttcatccgat tttttcaacg ggggtgaacc gatatacgcg
     1021 atggcttatt ttaccgcaat ggtcaaggat ttacccaaaa tgtaccgcac ggccaatcca
     1081 gtggtgcgta tcaaatatta tgtagatgtg ccggcggatg atgcctatgc cgtaccaacc
     1141 gctacgactg aatatcaaat tatggcttcc aataaaacct tgatggaagc tacgaccttg
     1201 gaagtggaag tggctcccaa tgcggggcaa accgatcgtc cagaagtcgg cgctcaagcc
     1261 gctaagatgt tatctcaact atccccacgt ttgcactcaa tccgagtggt attgagtatt
     1321 gataatgaaa actttgcaac cggatcgttt gagcttgatg gttcgcaagg gttagaaaaa
     1381 tttgcagctt tgggtgacga cttaattaaa tctaaaatac agaatgcccg tccgcctaaa
     1441 ggaggagggc tcgaaaaaac gattgaacaa ggcattcgtt ccgctttgaa gtcagaagga
     1501 tttaatgtgt tacgtgtgat tcaaacggat ccgcaatggt actatgaacg acacccgatt
     1561 agcggtgcga tacttaatcg tacgatacaa gccgccatta tttataaaga cgaacagaat
     1621 aagtgtatcg tcgaatacat ggagtttact cagaaatata tcaacagcca atacgatgct
     1681 ttataccgca gcatgcgcgg tactgtgaca aagccgtacg aagtgttgcc tgaacatgtg
     1741 aacgagtgag agaatgttaa taaagcagaa tacgtataaa caagtgatct aaaaagaagt
     1801 gaattaataa tgatcaagat tttactgtcg aaatggcctc gtattcattg gcatgttttt
     1861 ctgttgggtt ttgctatact ctcctgcgat agcaaagaaa caaacgagga taaagtagag
     1921 cctgttaatc catatacaga cctgcgttgg cgagtttctt taaccaaagt agctaaggcc
     1981 attactagca atccggtcaa tcccaataca atctacgtta atacttatag tgattatgta
     2041 gggcgcggtg gaatttttat tagttatgat cgcggcgaaa cgtggcctgc cgctatgtct
     2101 cctacaattg acacatcaac gaatcaggga gcgggcgatg tacagtttat agccgtatgt
     2161 cctaatgata ctcatatcgt ttttgcggga ggcgttcatt catcgggctt gctccgaaca
     2221 tcaaatggcg ggaagaattg ggttaacgtc ctaccgggtg ttgatgccga tgccgaggcg
     2281 atggctttta atcctcaaaa tccggcacag gtttttttca tgagttatac agatgccggt
     2341 ttttatgtaa gtcaggatac gggacggacg tggcagttgc gtaaatttac gggtttcgga
     2401 tattcgtgta ctctacgaat gcatccttcg gatccaaata ttttcgtagc cggagcttct
     2461 aaaggaaaaa taataaaaac caaagatcag ggttatagtt ggtacttagt acagaaacaa
     2521 gtgtccaatc aacctaatga gtggcctgag gtacctgcaa tcatatggga taatgaaaat
     2581 ccaaataaac tatatgctac tatttccggc gctaatggaa aatccttttc ccaaagtaat
     2641 aatggcggtg aaacatggca tataaaggat ttgaaagaag agttatgggc tgcgcatatg
     2701 acgaagagta aacttgttat attcggagga tggcaatcca cttatatcac cagtgatcaa
     2761 ggaaaaaatg tgtttaaaat cggaggtgat gaaacggtgt ccgggtattg gtccatcaca
     2821 tcatctggaa ataggatttt ggcgattagc tcgtacgatc gcagtctcat gatggttgac
     2881 ttataatgcg atgttgaaga ttattataac ttaataacga aagaattttc tatgaaattg
     2941 ttaatacgtg gtattttaat aatgctgttg gtttctggtt gtagtaaaaa tgaacaacag
     3001 gaaaatgccg ataaattcgg cgaggggatt actctcaaaa aagtggtaag gatgccggaa
     3061 gtgttaaata atcccgaaac atacgacggg caagaaatcg tagtagaatc aacgataaaa
     3121 acggtatgcc aagctcgtgg ttgctggatg gaagtcgaat cgggaatatt gccaatgcgt
     3181 atctcattta aaaatgatag ttatttcatt cccaaagatg cagcccggcg taaagtaaaa
     3241 atgcaaggcg tattgaaagt caaagaagtt tccgaagaag tggctcgtaa ttatctcaaa
     3301 gaagcaggac gttctgatga agaaatcgga aagataaacg gggcacaaag acttcttacc
     3361 atggtagcca gtggtttggc tatttataaa taatttgatt tgaccgtcaa tattcttatc
     3421 accacttcgt gatagatggt tgtagctcat cgttacagtt cattattaaa aggagtctaa
     3481 tatgaaatat atcttagtaa tagcattact tgtttctctt ggcagacaat ctttggctca
     3541 aacagaaaca attgaacacg gtgttggttt ttatgtcaaa ggtggctggt tcttcgatcg
     3601 tagttccgga tcagaagacc attcccctga attaaaaggt ctcaaaggcg agctcggaaa
     3661 tgacatatcg gatcgctcgg tgtatattgg agtgcgatat aaaaaaattg aggcagaaat
     3721 gggctgggga gaaatcataa aaaatagtga tattacgatt tatcccgttg aatcacgagc
     3781 tgcgcgttat tgggccggat ctgttgcata ctatgtgcca tttcaaattg gctcatcgaa
     3841 tccgatcaaa tccaaattag ctttgggtgt aaaatactcc acgtacaata ctagctatgt
     3901 ggatgagagt atcacgaaac tatccattga taaatggaat attaaatcca cctggtttcc
     3961 ggaacgaagc tatttcagtt ttctctttcc aacggtttcc tttttttatg aaaatcaaca
     4021 gaaactagct aatcagtcgg agttcggtcc tgcgcatagt attggcattg cgcttaaacc
     4081 ggccgccgtg tttcccttgg gtattttacc ggtaaaaatc gtgattgaac cgggaatgtc
     4141 gtggagtcat aaattatcag gtagcgatca tttccaaaaa gtttatgata ccgtgtattc
     4201 tttagatgct gcgatagtat acaatcttaa ataatcttag ataatgtttc agtagtttgt
     4261 atggagaaac tgtgtatggg agaattgaat ttcttggcta tcgcggttgc ggggtttgca
     4321 tattttatgt tgggcgcttt atggttttcg ccatttgtag ccggtacata ttatgataaa
     4381 gcgctgggat ttacacgaac caaagatatg aaatggggcg ttatctatta tattggtccg
     4441 tttatgggat gcgtaatttc aacctttgca acagccgtgc tgatgcggtg gtgttccgtt
     4501 ataagcatgg gagaagcgac aaagttgggt ttaatcgttg gtatgggata cggtatggtt
     4561 atttccatta ccaatgccat taacccaaat acgcccaggc cactcatgta tggtttggcg
     4621 acaggcggtt atcatgtgat tggaaatatc atggtttctg tgttaattta tattatgtga
     4681 tccaatcagg ttgcattggt ttatggagaa tcaaatttta aaaaaattaa tttatgtcct
     4741 gatattacaa tcatgatttc catccgcttg atctcactgt tagttgcttt ttttattaat
     4801 aaatcttttt ttgatgtaaa caaaactttg atggcctttt cagtaaataa atcaggtgtt
     4861 atgtcagccg attataatcg gttttcagaa aagtatggcg atcatttgtc gcaagaaggc
     4921 gatctgtata tacccatcgg gcaacccaaa gccatagtgt gtttttttca tggaggattt
     4981 tggcgcgccc cttatgggcg tgatcagttt gatagcgtag cgattgattt tgctcaagcc
     5041 ggctatatta tttggaatat cgaataccgc cgtgtgggtg aagtcggcgg tggttggccg
     5101 ggtacatttg aagatgctat tctatctgta aattatctta gcacgttaca aaaaaagtat
     5161 cgttggagcg ataatttgaa tgttgtactc gccggtcatt cagccggcgg gcaattagcg
     5221 ttgtggttaa cagctcacgc cgaaaaaaaa tttcttcagc atacgccatt gaccatacat
     5281 ccggatttat gcatagggct cgcgccggtc gtaaattttg aaaaagcgta tcaggaaaat
     5341 cttggagggc aagcgattta taaattactc ggttgtactc caaaggaaaa tcctgaacgg
     5401 tatacatgtt atgatccgtt gtttgctgta catctaaata cgccgcaaat tatcttgcat
     5461 ggcgagatgg atgaagactt acctattgcg tattccatat cttaccttcg tgcagcagaa
     5521 gcccggaaca cctctattga tctgattgag ctggaaaaaa ccgggcatat ggatttttta
     5581 gaacctcaga gtttggccgt aaaacgatgg aaaatgttac tttacctgcg ctatggaata
     5641 cataaataac taattgatca gtaaggctat acacgaactt atgtgatttt tcttctgaat
     5701 aaccctaact gatctgaaaa ttttatatga gtttgctaag aattatagca aagcctttgc
     5761 ccgatgagta tccgatgtat tcggctaaat acattgatct attaaaaaat gacggccttg
     5821 ttttatatca tctgatgaat aattttcttg agattcgtca caacattacc gaactctctt
     5881 cagaaattct gcattatcgt tacgcgccgg aaaaatggaa tatcaaagag acgttggttc
     5941 acatgatgga tgatgaacga atatacgcat accgagcgct ttgctttgcg aggggcgaga
     6001 aacaaaaatt gcccggtttt gatcaggata gttataccgc attaagtggc gcgagtgcac
     6061 gaagtatcca gagtattttt gtggaatacg aagccgtgcg tcatgccacc gtggctatgt
     6121 ttaatgcctt taccgacgaa atgttattgc gcatgggata ttccgatgaa aataacagtc
     6181 atcgctctgt gcgtgctttt gtttatcata ttgccggcca tgagttacgt cattgggaaa
     6241 tcatcaaaga acgttatttg gatcgctcgg cgagttgcgg tacagtctaa cgaaatgagt
     6301 tacttgtttt aatcttaatg gaaaccgaaa cgtttaaaat tgacattcag aatattctcg
     6361 gtaaagattt tgccgaacat gaactgattc attcgtttta tggtgaaacc tttgcattgg
     6421 aagatcaatt ctttcctccg gttgtttcct atgcactacc acgcattttg cagtgtgaaa
     6481 gcgccggtgt tttagcatgt tcattgccgg atgaacatgc agaacagatg ttgcactatt
     6541 ggtttggaag taaagattca gcgtatattt catccggaag catacgcgta gcatcgcatt
     6601 gtaattcatg cccggatagc tgcacgggta tttgccccgt attgatgcgt gaagatccgc
     6661 aatatttttt acctccgtat catgtgcgag gttatccttt ttcaggctcc atacgaggcc
     6721 atattgtatt aagcgacccg aaacccttga ggatacatcc ggaaggtgag cggctgatta
     6781 ttcgaaccac caaacggttg tttaatcaat tcaagatgca atttcatgtt gatttaggtg
     6841 cagatcacca agcggttgat gcattggttc cgatttgctc tttttgtaaa aaaataaggg
     6901 atgatgaata tcaatgggtt cgcttggaaa aatttttcaa cgacaattat agtattcagt
     6961 ttacgcattc tatctgtccg cattgttctc atgagtatat aaaaaccata aaataatttt
     7021 ctgaaaaatc tcttaaagtg atgctgattg ttctatataa atttgcatct ttttgttgtt
     7081 aggatcgtac attgatagca attacataag tgaattgtat gttatgaaaa gtatgttttt
     7141 atcttacttt gtttgttttg catttgtgag ccataccttt gttgtatcgc aaataaaacc
     7201 ctcggatcgc gttatttcaa aagtcgtgtg ccaatatgat acgtcgcaat cctatgtact
     7261 ttttttacct tcagggtatg attcgtctcg aaaatatccg attctatatt gctttaaacc
     7321 cgaaaaagat gcgctgtatc ccgtagtata ttatgccgat atcatggagc ggtacggcgt
     7381 tatcatggtg tgttcatgga attcgcaaaa cggtccgtgg caagtatgta ttgatgcgtt
     7441 gcatgcggtt tggaaagata cgcatcatcg cttttcgatc gatctgggtc gtgtttatgc
     7501 gaccggtttt tcgggcggtg cgcgtacggc gtcggaatta gccatacggt atccccatca
     7561 ggtcaccggc gttatcggca taggctcggg ttttagtaca ctccaagcga taccgcaagc
     7621 cgtacctttt cgatactttt atggtctcta tggaaatcgc gattacaaca aaagtgaaat
     7681 gatctatctc gacgaagaac tatccaaacg tcaaatgatg caccgcatcg tcgaatttga
     7741 aggcatacat caatgggctc ccttcactga tttttcagat gggttcgaat ggatgatgtt
     7801 aaatgaaatg cgtgacggga aacgaacacc ggatagcgca tggattgctc aattatttga
     7861 caaacggcat gaacaattga aaacatcact tcatcaaaaa aactttgttg atgcggcacg
     7921 gatggcgcgt gccatatatg atgattttca atttacgatg ccttgctctg catttgttgc
     7981 actggccgat agcgtacaaa aattaaaagc ctattctgac gaaatggctt tgcgatccga
     8041 tttgcagcaa cgcgaagtgc acacgcaaaa tattatggac gattttttgt ttaatttgga
     8101 aaatcctttg tttgaaccgg aacgggacat gcagcttcag atgttgtaca aactaacgaa
     8161 agaatggcaa ccgctatata acaccaaaaa tgaaataaag agtaatgcag cgtaccgttg
     8221 tttagattat atagcacgtc tttgccgagc gcgcggtaac ggatatttaa atcaacgtgt
     8281 cttgccaaaa gccgtcgctt gttttgattt ggcgcgaacc gtatggccgg atgacgcctc
     8341 gtcgcattat aatttagcat gcgctttggc tttatccggt aaaacaaaca aggctttaga
     8401 tgctcttgaa acggccgtaa aagttggatt taatgatgtg ccgttacttg atacggatcc
     8461 cgatttgaat agcgttcgtt ccgataaaaa atttaatagt attaggaaga aagctgagca
     8521 ttctaccaca tctaaaaagt aattttacga taaccgaatt tcttgcctta tttctgacaa
     8581 aggcgtaggt tattgtgaaa tagtatggga tgtgatgtat gaaatggttc tatgttttgt
     8641 tgtttgtttt tgtagtcgtg ttctcatatt gcttgctcca agcgaaacaa gccgtaatgg
     8701 atacttcatc tattgagcaa gccgtgctca attaccatac gtatgctgaa tggctcacct
     8761 acggcgtacc ggcggctttt ttgattttac ttatacttgc caatttttca ttcac
//
LOCUS       DAOQNQ010000121         8809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2948_length_8809_cov_5.718643, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000121 DAOQNQ010000000
VERSION     DAOQNQ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8809
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2948_length_8809_cov_5.718643"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PK754_08665"
     CDS             complement(<1..369)
                     /locus_tag="PK754_08665"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB09616.1"
                     /translation="MGFSNIFKIAFNALMRNKMRSALTMLGIVIGVGSIIAMTGVGAG
                     AQKEIDNQTAAFGTNMITIRSGGMTAGGIRIRAMKELQPSDGDAIVRGSELVTAQTPV
                     IRLVTNVVSSERNWASQVYGV"
     gene            complement(381..1181)
                     /locus_tag="PK754_08670"
     CDS             complement(381..1181)
                     /locus_tag="PK754_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015781706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB09617.1"
                     /translation="MSQPTQQIFHPLMERAGSKPLLSVRNIKKTYRMGDIEVMALRGV
                     SLDIYEGEFVAIMGSSGSGKSTFMNTLGCLDQPTSGEYLLDGVSTGHMTKTDLARIRS
                     FKIGFVFQGFNLLSRTSAIENVELPMLYQGVAPKERHDRAMEALKAVGLEKRYDHKPN
                     ELSGGQQQRVAIARALAAHPSMILADEPTGNLDSKTSIEIMQIFMRLNDQGITIVLVT
                     HENDIAQYCKRNVVFRDGRIIKDFMVERRLEPEEEMRKVLATSIVDNA"
     gene            complement(1195..2352)
                     /locus_tag="PK754_08675"
     CDS             complement(1195..2352)
                     /locus_tag="PK754_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB09618.1"
                     /translation="MNKKIWIIGGIAVLVAVILGWIVWGGSTAAGGYKTEAVTRGDIS
                     VVITATGKVNPVTRVQVGTQITGTISRIFADFNQKVRKGQEIAEIDPTFLKAQLLEAE
                     ANAEKAKAQVEQSKKTLDRATELFDRKLISQSEKDEALTNYNLAGAQYKQAMAAYNRA
                     EVSLRYTSIVSPIDGVVISRNVDVGQTVAASLQAPILFVIANDLSKIQVEATIDEVDI
                     GKVKVNQEVSFYVDAYPDEKFKGRVAQVRLQPITTQNLVSYEVIIEVENRDNKLLPGM
                     TANLSIIVDTKKDVIKVPNMALRFQPALTQEQFKKYSAMYGDKFDLKNSAMVWTLQSG
                     ELAPIIVDPGLTDGQYTEIRSDQLTIEQILVTGISTVAAPGSARDRFAPKK"
     gene            complement(2372..3727)
                     /locus_tag="PK754_08680"
     CDS             complement(2372..3727)
                     /locus_tag="PK754_08680"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNB09619.1"
                     /translation="MRCMKFIFVWVILAARSGWSQEVITISTEDTLTLDRCIEIALKN
                     QPALGAAQGAIITAESDYTQVRSLRFPQIQMEAGAYVTNTPLGSSNAIAQAPVIEPKN
                     NTDFIPTIRLTVKQPIYDFGRTDKSLESKSKLIRAAEMSLQATEDDVILNVHTSYYNY
                     VLAQQILKINEERVTQSRKHLERARGFFAVGKIPESEVYKAELEVSNAELELTNARGK
                     LQLAKVSLNSAMGVADWTDDVTSYKVASHENYVAFNANLRESINSAFTGRREVAASEM
                     RVQAWRSALSAAKSQYYPIITASGGFGPYIVRNTLTTGETKDKFKIGYNIGVNFEFPI
                     FQGLNVRADISEAQGGIRIASSQYNTTKQRIAQEVQERYYSVKFAEERYKSTERIVTQ
                     TERNLNLAEGRFDTGVGSAIEVTDANFSVANARIERTTALYNYKIELARFQRTIGTLR
                     K"
     gene            complement(3875..4654)
                     /locus_tag="PK754_08685"
     CDS             complement(3875..4654)
                     /locus_tag="PK754_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003333918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNB09620.1"
                     /translation="MLFDTHAHLDYDLFDDDREAMIARAREAGVDTIVTIGTNLQSAE
                     RAKTIAEKYDFVFFAVAHHPADAHTYQSSMMPHYEKLIQHPKCVAVGETGLDYYWKEA
                     APEQQLPLFRSFIRMACVYDKPIIIHNREANHDIISVLQEEKQNGKLSNLRGIMHCFS
                     GDAEAVKQALDLNFYISFSGNITYKKSHLPSIIPMIPHDRILIETDAPFLTPVPHRGK
                     RNESANLRFTAEKLSEINKWTLGETESITSANARRIFGMTH"
     gene            complement(4659..>8809)
                     /locus_tag="PK754_08690"
     CDS             complement(4659..>8809)
                     /locus_tag="PK754_08690"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNB09621.1"
                     /translation="KLALRNTLKTIIGTVVSIVLLIGLVLAAIQLPWVKNILRGEIER
                     IANKSLYAKLTIGKIEGNFLTHISVFDGRIAFGDTSVLAFESVDVALSPLAIFNKQIQ
                     VDHIRIRKPFIHAIGDTNGVINLARLAKPIEPEPVDSAAEPLAGFTINLDEFSIEEGH
                     VKVQLPAFDGALQNLNVRMNVKASPNKQNIKLDQLAFLLTKNKLNKAKETVKTDSLDI
                     KNIGLDVSAHLLARSLYYQINHPEKRDSLWLDINEFRFVTDQSDVYVKGRFLLPDSAR
                     GIALHYTADIKTQPLHLSDIRRFAPVQMRNIDKIEFEALATGNDRAATISDLRIHTPA
                     GTLRGFADVHFGADEPLRYVTELEFLGINAAAFADNPDLFTNINGHIRAEGAGIEPKT
                     LTSKLELQLVKSKAYGIGIDRFTINADVQNGKAQLTKFEGITTAGNFNCDGYYNLIDE
                     TYHLQTRLRSINIADVVGDTSLQSSINLSLAYDGKGLNPKTAKGQVRVVSDSSKILGR
                     ELQNLTFDASQQNGRLTLNEVKLRTPLANVTASGSVGLDSSVNLKYTMETVDFTLLKK
                     YLGNDSLLKDSLNLKLKYNGSLRGTPNKMTTAGFLTLTNFIFSGITIDSMQFSYFFDN
                     LSPNQLARALDFRREDTTIIGDVFLYTHMVNMEGMVFRDFTTSITKEKGRTLFEVSGS
                     QDTADAFASVRGSLVLENEKRGELFFDELFLKINGKSMRTREVSQKLGEDATIDTTFE
                     RWTESWKNSQPVDIFFDIEKNQYEFRNFNLDIGRGSVSLAGKADIAGDQNLDIKLKDL
                     DLSRANALIGSKQSVVEGILNMTASLKGTFEKPVILADWSITKGKASEFTYENFLGNM
                     QYLNRKIQVNMTLNQNSEKSLTMGGYVPIDLSFKDVKERFTNRPMNFKMHSEGIDLRF
                     LQSFFGRSLRLNRGDLKVDMTISGNKEKPKLEGEVKIEGGQITFPRSTLGQTFRNISM
                     YVKLTPERIFLDTLFVQSGKDSRSRLFAQGSVDLGDVLKDFKFENVDKISYLINAHFT
                     DFVPINTKSETSYLHTASISGKMTVKAPTLYYTQISGDLVINNSEIWVVDPRKAKLAT
                     ASAPAKKGEKHSIDFYKNLDLDINIDLPENADNAIRSAEMLLTLYGNIGVTKPPESEE
                     FFLSGDVKTRPGGKYAYLSIAFNIEKGDIVFTGEPGVNPNLDIVAKKRFEYKDSEGQS
                     IPAEARIQVTGKLLKPEVAISAVERGTEDSPLPDLTEPADILSYLVLGVKTKDLAKVD
                     ASQAKDIVQQTAINQVLNIVANKAGLSKLEYIPSTSDASKATIEVGKRISETVSVNFS
                     GGTDPTVGRSITLEVVADSVWGVRKFLKSWKKTLEFEYKQPPQEKTDQPDIFNLLLYF
                     RKEY"
BASE COUNT         2326 a         2075 c         1820 g         2588 t
ORIGIN      
        1 gacgccatat acttgagatg cccagttacg ttccgatgaa acgacattag ttacaagacg
       61 aatcaccggc gtttgtgccg tgactaattc cgacccgcga acaatagcgt cgccatcact
      121 gggttgtaac tccttcatcg cacgtattcg gataccgccc gctgtcatac cgccggaacg
      181 aatcgtaatc atattggtac caaaggcggc cgtctgatta tcaatttctt tttgcgcacc
      241 ggcacccaca ccggtcatgg caataataga accgacgccg atcacaatcc ccagcattgt
      301 aagcgccgaa cgcattttat tacgcataag cgcattaaat gcaattttga atatatttga
      361 aaagcccata tcaattcctg ttaagcatta tcaacgatgg aagttgcaag taccttacgc
      421 atttcctctt cgggttcgag acgtcgttcg accataaaat ccttaataat acggccgtca
      481 cgaaaaacaa cgttgcgttt acaatattgt gcaatatcgt tttcatgtgt cacgagtacg
      541 atcgtaatac cttgatcgtt gaggcgcata aaaatctgca taatttcgat actggtttta
      601 ctgtccaggt ttccggttgg ttcgtcggca agaatcatag atggatgtgc cgccagagcg
      661 cgcgcaatcg caacgcgttg ttgttgaccg ccggaaagtt cattgggttt gtgatcataa
      721 cgtttttcca agcctaccgc tttaagcgct tccatggcgc gatcgtgccg ttctttggga
      781 gctacgcctt gatagagcat cggcaattcc acgttttcga tagcactcgt acgcgacaat
      841 agattgaatc cctgaaaaac aaatccgatt ttaaaactac gtatgcgcgc cagatcggtt
      901 ttggtcatat gacctgtgga tacgccgtct agaaggtatt ccccggatgt cggttggtca
      961 agacatccaa gcgtattcat aaacgttgat ttgcccgatc cggaagatcc catgatcgca
     1021 acaaattcac cttcgtaaat atccagcgat accccgcgta aggccatcac ctcgatgtca
     1081 cccatccggt atgttttttt gatgttgcga acggataaca atggtttgga tccggcacgc
     1141 tccatcaaag gatgaaatat ttgttgtgta ggttgactca tatttaatga tgatttattt
     1201 ctttggtgca aagcgatcgc gagcactacc tggcgccgca acagtgctga tgccggtcac
     1261 caatatctgt tctatcgtaa gttgatcgct acgaatttcg gtatactggc cgtccgttaa
     1321 ccccggatcg acgatgatcg gcgccagttc gccggattgt aatgtccaaa ccatcgcgct
     1381 gttttttaga tcaaatttat caccgtacat tgccgaatat ttcttgaatt gctcctgcgt
     1441 cagcgccggc tgaaatcgca atgccatatt tggaaccttt atcacatctt ttttggtatc
     1501 aacgatgata gacaaattgg ccgtcatgcc cggcaacaat ttattatcac gattctctac
     1561 ttcgatgatt acttcgtagc taaccaagtt ctgtgtagta atcggctgca aacgaacctg
     1621 agccacacga cccttaaatt tttcatccgg atacgcatct acataaaaag acacttcttg
     1681 attcactttt actttgccga tatccacttc atcgatcgtt gcttcgacct gaattttgct
     1741 gagatcgttg gcaatgacaa aaagaatcgg cgcctgaagg cttgcggcga cggtttgtcc
     1801 gacatccaca ttgcgcgata tgaccacacc gtctatcgga gagacgatgg atgtataccg
     1861 gagcgatacc tccgcacgat tataggcggc catagcttgt ttgtattgcg ctccagccaa
     1921 attgtaatta gtcagcgcct catctttttc cgattgggaa atcaattttc gatcaaaaag
     1981 ttcggtagct cgatcgagcg tttttttcga ttgttctact tgcgctttgg ccttttccgc
     2041 attggcttcg gcttctaaaa gttgtgcttt taaaaatgta ggatcaattt cggcaatctc
     2101 ctgccctttg cgcaccttct gattaaaatc ggcaaagatg cgtgaaatcg tgcccgtgat
     2161 ctgagtaccg acttgcacac gcgtgaccgg attgactttg ccggtcgccg tgatgaccac
     2221 ggaaatatcg ccgcgcgtaa cagcttccgt tttataaccc ccggcagcgg ttgatccgcc
     2281 ccacacaatc catccgagga ttacggccac aagcacggca atacctccga tgatccatat
     2341 tttcttattc atgtatagct cctaaaaaca attattttcg caaagttccg attgtgcgct
     2401 gaaaacgcgc cagttcgatt ttgtaattat ataatgccgt tgtacgttct atgcgcgcat
     2461 tggctactga aaaattggca tccgtcactt cgatggctga accgacaccg gtgtcaaatc
     2521 gcccctccgc cagattgaga ttacgctccg tttgagtaac tatacgctcg gttgatttgt
     2581 atcgttcttc cgcaaacttc acactgtaat atctttcctg cacttcctga gcaatacgtt
     2641 gtttcgtagt attgtactgc gaacttgcaa tacgaatccc gccttgcgct tcggatatat
     2701 cggcacgtac attgaggcct tgaaaaatcg gaaactcaaa attcacgccg atgttataac
     2761 cgatcttgaa cttgtctttt gtttccccgg tagtcaatgt gttacgcacg atatatgggc
     2821 caaaaccacc cgaagctgtg ataatgggat aatattggct tttggctgca ctcaatgcag
     2881 aacgccatgc ttgaactcgc atttccgaag ccgctacctc acgacgcccg gtaaacgcag
     2941 aattgataga ttctctgaga ttcgcgttaa aagctacata attttcatga gacgccactt
     3001 tataacttgt tacgtcgtcc gtccaatcgg ccacgcccat agcgctattg aggcttactt
     3061 ttgctaattg taattttcct cgcgcattcg tcaattcaag ttcagcgtta gaaacttcaa
     3121 gctccgcttt ataaacttcc gactctggaa tttttcctac ggcaaaaaaa ccacgcgcac
     3181 gctctaagtg ttttcgcgat tgcgttactc gctcctcatt gatttttaaa atttgttgcg
     3241 caagcacgta gttgtaataa ctcgtatgaa cattgagtat cacatcatct tcggtagctt
     3301 gcagagacat ctcagcagcg cgaattaatt tgcttttgga ttcgagcgat ttatccgtac
     3361 gaccaaaatc ataaatcggt tgtttgacgg taagtcgtat ggtgggaata aaatccgtgt
     3421 tgtttttagg ttcgatcacc ggtgcctgag ctattgcatt ggaagaccca agaggtgtat
     3481 tggtcacata agcccctgct tccatttgaa tctgcggaaa acgaagtgag cgcacctgcg
     3541 tataatcgga ttcggccgtg ataatagcac cttgcgctgc accgagagcc ggttgatttt
     3601 tgagagcaat ctcaatacag cgatcaagcg taagcgtgtc ctctgtcgaa atggtaataa
     3661 cttcttgcga ccatccgctc cgcgcggcca atattaccca cacaaaaata aatttcatac
     3721 aacgcatgtt ttttcctttg agtaacgatt cagtctttga tctacaaacc ccatgccaaa
     3781 cgttattttg ataaacccat attttacacc gtgaaaatac taatattaat ttctattgca
     3841 atacttcaca tgcttaattt tcacagtgtg aaattcagtg ggtcattccg aaaatgcgtc
     3901 gggcgttagc tgaagtgatc gactcggttt cacccaatgt ccatttgttt atttcactta
     3961 acttttcggc cgtaaaacgt aaatttgccg actcattacg ttttccgcgg tgcggaaccg
     4021 gtgtcaaaaa tggcgcatcc gtttcgatca aaatacgatc gtgcggtatc ataggaatga
     4081 tggaaggcag atggcttttt ttataggtaa tgttccctga aaaagagata taaaaattca
     4141 aatccagcgc ttgttttaca gcctctgcat caccggaaaa gcaatgcatt attcctctta
     4201 aattactcaa tttcccgttt tgcttctcct cctgcagtac gctgatgata tcgtgattgg
     4261 cttctctgtt atgaataatg atcggcttgt cataaacaca cgccatcctg ataaaagaac
     4321 ggaatagggg gagttgttgt tccggtgccg cttccttcca ataatagtca agccccgttt
     4381 cgccgacagc cacacacttg gggtgttgta tcagcttttc atagtgcggc atcatagatg
     4441 attggtatgt atgagcatcg gcaggatgat gcgctacggc aaaaaaaacg aagtcatact
     4501 tttcagcaat cgttttagct cgttcagccg attgtaaatt ggtaccgata gtcacgatcg
     4561 tatctactcc cgcttcgcgc gcacgtgcga tcatcgcttc gcggtcatcg tcaaacaaat
     4621 cgtaatccag atgcgcatgc gtatcaaaca acatagaatc agtattcttt ccggaagtag
     4681 agcagaaggt taaaaatgtc gggttgatcc gttttttcct gcggtggttg cttatattca
     4741 aattcgagtg tttttttcca ggattttaaa aatttgcgaa caccccacac gctatcggcc
     4801 acaacttcca atgttatgga tcgtccgaca gtagggtccg taccgcctga aaaatttacc
     4861 gaaactgttt cactgatgcg tttaccgacc tcgatcgttg ccttgctcgc atccgacgtg
     4921 ctgggtatgt attccaattt tgataatccg gccttgttgg ctacaatatt caacacttga
     4981 ttgatcgctg tttgctgcac aatatctttt gcttgcgacg catccacctt ggccaaatct
     5041 ttagtcttta caccgagtac caggtaagac aaaatatccg caggctctgt caaatccggc
     5101 aatggtgaat cttccgttcc gcgttcgacg gccgaaatgg ccacttccgg tttgagtaat
     5161 ttaccggtta cctgaatgcg tgcttcggca ggaatacttt gaccttccga atctttgtat
     5221 tcaaatcgct tcttagcgac gatatccaaa tttggattga ctccgggttc acccgtaaat
     5281 acgatgtcgc ctttttcgat attaaacgca atactcagat aagcatattt cccacccgga
     5341 cgcgttttca cgtcaccgct taggaaaaat tcttcggatt ccggtggttt ggttacaccg
     5401 atattcccat agagcgtaag taacatttcc gcgcttcgaa tcgcattatc cgcattttcc
     5461 ggtaaatcaa tattgatatc taaatctaga tttttgtaaa aatcaatgga gtgcttctcg
     5521 ccttttttgg ctggtgctga cgccgtggct aacttggctt tacgagggtc cacgacccaa
     5581 atttcagaat tattaatcac cagatcgccc gaaatctgcg tataatatag cgtgggcgct
     5641 ttaactgtca ttttacctga tatagaagcc gtatgaagat acgaggtttc tgatttcgta
     5701 ttgataggta caaaatccgt aaaatgagcg ttaatgaggt agctgatttt atccacgttt
     5761 tcaaatttga aatccttgag aacatcgccg agatccaccg aaccttgcgc aaaaagcctc
     5821 gaacgcgaat ctttcccaga ttggacaaat aacgtatcca aaaaaatacg ttccggcgtc
     5881 aatttgacat acatactgat atttctaaaa gtctgcccca aagttgatcg cggaaaagta
     5941 atttgccctc cttctatttt gacttcacct tccagtttcg gtttctcttt attgccgctg
     6001 atcgtcatat ccaccttaag atcaccacga ttgaggcgaa gcgaccgccc aaaaaaagac
     6061 tgcaaaaacc gcaaatcaat accttcgctg tgcatcttaa aattcatcgg gcgattggta
     6121 aaacgttctt tgacatcttt aaacgaaaga tctatcggta catatccgcc catcgtaagc
     6181 gatttttctg aattttgatt gagcgtcata ttgacctgaa ttttgcgatt cagatattgc
     6241 atgtttccga gaaagttttc ataagtaaat tcggaggctt tacctttagt gatactccag
     6301 tcggccaaaa tcaccggctt ctcgaatgta cctttgaggc tcgccgtcat attgagaata
     6361 ccttcgacaa ccgattgttt ggaacctatc aaagcattgg cacgcgacaa atccaaatcc
     6421 ttgagcttaa tatctaggtt ttgatctccg gcaatatccg ctttacccgc aagactcact
     6481 gatccgcggc caatgtctaa attaaaattt ctaaattcat actgattttt ttcgatgtca
     6541 aaaaatatgt caacgggctg actgttcttc cacgactccg tccaccgctc aaatgtcgta
     6601 tcaatcgtcg catcttcgcc gagtttttgt gacacttccc gtgtccgcat ggacttaccg
     6661 ttgatcttga ggaagagttc atcaaaaaac aactcacctc gtttttcatt ttccaaaact
     6721 aaacttccgc gtacactcgc aaaagcatcg gctgtatcct gcgatccgga gacttcaaat
     6781 aatgtacggc ctttttcttt agttatagat gtggtaaagt ctctaaatac cataccttcc
     6841 atattgacca tgtgggtata caaaaaaaca tcgccgataa tggtggtatc ttcgcggcga
     6901 aaatccaatg cgcgtgctaa ttggttgggc gaaagattat caaaaaaata gctaaactgc
     6961 atggaatcaa ttgtaatacc ggagaaaata aaattggtca gagtcaagaa acccgctgtc
     7021 gtcattttat tgggcgttcc gcgaaggctc ccgttatact ttaatttcaa attcagtgaa
     7081 tctttgagta aactatcatt acccaaatat ttttttagaa gcgtgaaatc aacagtctcc
     7141 atcgtgtatt tgaggttgac tgacgaatct aaccccaccg aacccgaagc cgtaacattg
     7201 gccagcggtg ttcttagttt tacttcgttg agtgtcaagc gaccgttctg ctgactggca
     7261 tcaaatgtaa gattttgaag ttcgcggcct aaaattttag atgaatccga aaccacgcgc
     7321 acttggcctt tggctgtttt agggttgagg ccttttccgt cataggctag cgaaagattg
     7381 attgagctct gcagcgatgt gtcaccgacg acatccgcga tattgatcga acgtaaccgt
     7441 gtttgaaggt ggtacgtttc gtcaattaga ttataatatc catcgcaatt aaaattcccg
     7501 gctgttgtta ttccctcaaa tttagtcaat tgagctttcc cattttgcac gtccgcgttg
     7561 atcgtaaagc gatcaatgcc aatcccgtaa gctttggatt tgactaattg gagttcgagt
     7621 ttactcgtga gagttttagg ctctataccc gcaccctcag cacgaatatg tccattgata
     7681 ttggtaaaaa gatcaggatt atccgcaaaa gcggccgcat tgatacccaa aaactcaagc
     7741 tcggttacat aacgcaatgg ttcgtcggcg ccaaaatgta cgtctgcaaa tccacgtaat
     7801 gtcccggccg gtgtatgaat gcgcagatcg ctgatggttg cagctctatc attgcctgtg
     7861 gcgagcgctt caaattcgat cttatcaatg ttccgcattt gaacaggtgc aaacctgcga
     7921 atatccgaaa gatgcaaagg ctgtgtttta atgtcggctg tataatgtaa agcaataccg
     7981 cgcgcactgt ccggtaacag gaatcgtcct ttgacataca catccgactg gtcagtaaca
     8041 aatcgaaatt cattgatatc caaccataac gaatcccgtt tttccggatg attgatctga
     8101 taatacaatg aacgcgctaa taagtgcgct gaaacgtcca atccgatatt tttgatatca
     8161 agcgaatccg ttttgactgt ttctttagct ttgttcaatt tgtttttagt caaaagaaac
     8221 gctaactgat ccagtttaat attttgttta ttgggtgacg ctttgacgtt catacgtaca
     8281 ttaagatttt gaagtgctcc gtcaaaagca ggtagttgaa cttttacgtg accttcctct
     8341 atcgaaaact catccaaatt aatggtaaaa ccggccagag gttccgccgc gctatctacc
     8401 ggctccggtt ctatgggttt ggctaaacgt gccagattga ttaccccgtt ggtatcgccg
     8461 atagcgtgaa taaaaggttt acggatacga atatggtcaa cttgaatctg tttattgaaa
     8521 atcgccaaag gagaaagggc cacatctaca gattcaaacg ccaatacgga ggtatcaccg
     8581 aaagcgatcc gcccgtcaaa tacgcttatg tgcgtgagaa aattaccttc gattttgccg
     8641 atagttagtt tcgcgtagag tgatttatta gcaatgcgtt cgatttcccc gcgcaaaata
     8701 ttcttgaccc atggcaactg aatggccgca agaacaagcc cgatcagaag tacgatagat
     8761 acaaccgtac cgattatagt tttgagagta ttgcgaagtg ccagtttta
//
LOCUS       DAOQNQ010000122         8789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2960_length_8789_cov_5.179528, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000122 DAOQNQ010000000
VERSION     DAOQNQ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8789
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2960_length_8789_cov_5.179528"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PK754_08695"
     CDS             <1..591
                     /locus_tag="PK754_08695"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNB09622.1"
                     /translation="KKDSSTNPKEACRQSGVQQVAAGYIIYGSSTMFVYTAGHGVHGF
                     TLDPGLGEFILSHENIKTPERGKIYSINEGNSNKWHDGIRNYIAYLKEENKSDKRPYS
                     SRYIGSLVADFHRNLLYGGIFLYPADRSNPEGKLRLLYEANPLAMIVEAAGGAASDGH
                     RRIMEIEPTALHQRVPLIIGSKADVETACAFIQGQR"
     gene            complement(595..1401)
                     /locus_tag="PK754_08700"
     CDS             complement(595..1401)
                     /locus_tag="PK754_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB09623.1"
                     /translation="MEIPAHERLALTGVSGSGKTTLLRMLAGLTEPDSGRIYWQDQPW
                     FDSEKKINRPPERRAVGFVFQDYALFPNMTVYENLAFALSEPDDEAVRRMLQLTELEG
                     LASRRPDSLSGGQKQRVALARALIRKPVLLLMDEPLAAMDVALRKKMQQEILTLWNEA
                     PSMMVWVSHDMAEICRLSNRVCVLDNGRMVKLGNPMDVYHDKHLSGKFRMDGEVVGME
                     PNDTIFILTIASGERLIRVVATASDIESLQIGDRVTVVSKAFNPIIIKQN"
     gene            complement(1467..2135)
                     /gene="modB"
                     /locus_tag="PK754_08705"
     CDS             complement(1467..2135)
                     /gene="modB"
                     /locus_tag="PK754_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019597475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HNB09624.1"
                     /translation="MDWAPFWLTFKLAGCTTVLLLIAGIPLAYYLAFTKSKWRIPLQT
                     LVSMPLVLPPSVLGFYLLIAFSPKFWFGAAIESWFDVRLAFSFTGLLIGSFIFSLPFM
                     VNPLRTGFGSVHRSLIEAAQTLGKSRGEILYRVLLPNMRPAVVSASIMTFAHTVGEFG
                     VVLMIGGSIPEKTKVASIALFEAVEQMNYSMAHTYALVLVIFSWCVLFGIHVIESKNN
                     ARGR"
     gene            complement(2140..2544)
                     /locus_tag="PK754_08710"
     CDS             complement(2140..2544)
                     /locus_tag="PK754_08710"
                     /inference="COORDINATES: protein motif:HMM:NF015424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNB09625.1"
                     /translation="MNKIKCHIVAIETEASLSLVTMQSANAPLTAVIIETPETASYLK
                     PGVQVYAAFKETEVNLLPQDCRHGSMSNQWTGTIVQIRWGKLLTEVGVQTAIGTIRSV
                     LTTENAKRMSLEENHNLEVYVNAQAVAVWEDA"
     gene            complement(2537..3310)
                     /gene="modA"
                     /locus_tag="PK754_08715"
     CDS             complement(2537..3310)
                     /gene="modA"
                     /locus_tag="PK754_08715"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNB09626.1"
                     /translation="MPTSLQHLIKNLFANLCMLMVLNVFELHSQSSVRIAVASNVSPA
                     TKTLMAIFTQQTGIRVEIISGASGKLATQIEHGAPYDIFLSADTIFPNILYEKKLTRM
                     RPRLYAQGQLVAWSARPLYSDDAMGSIDQAEIRHIAIADPALAPYGKAAMDAMKKSGQ
                     YERVKDKLIFGENISQVNQYITTGTADIGLTALSSVLSAPTSLKGYYRVVDSKLYNPI
                     IQSAALLNDKSEARQCFEFIYSDTAKEVFEKFGYTRIHE"
     gene            3566..4573
                     /locus_tag="PK754_08720"
     CDS             3566..4573
                     /locus_tag="PK754_08720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HNB09627.1"
                     /translation="MTTSPILIQKRFQFPPGQNPFITEEQRFFSYNHYIRSRFGQRIH
                     RVTVDAGFTCPNRDGTVASGGCTYCNNDGFSPASQIYRVKKYLKPSTPVHDQITNHID
                     AMKKRFKTDKFFAYFQAYSNTYAPVDELETLYREALHHPDIIGLVVGTRPDCVDEEKL
                     QLLQSIAETHYVSIEYGCESIYDKTLQWVNRGHDFACFIDAVEMSAGRNLDVAAHIIL
                     GFPTETRDEMLAMAGAINRLPVDFIKIHNLHIVAQTPLAFQYKKSPFPVFEQHEYSML
                     VCDFLERLSPNICIQRLYGDAPRAMQIAPRWSTTGQDMNLWVQAELTRRQSFQGIRAT
                     E"
     gene            4658..5818
                     /locus_tag="PK754_08725"
     CDS             4658..5818
                     /locus_tag="PK754_08725"
                     /inference="COORDINATES: protein motif:HMM:NF037429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09628.1"
                     /translation="MISQKTIERLSKIGSRDFFITSLYLNLDHTQKHGQYKIALKDLL
                     KDRRHRLDEFRKHNKITKEQIKSLEDDFVRIENYIQHEFVSKDSGKGLAIFSCSATHF
                     WEILELPQPVPNHLNADFDPNIRILSELLCDYRHYAIALVDSSKAKIVDVNLGFVREV
                     WSVEHAITGKTKFGGVDGTQERNAERAHDESVHKHFKVVATEVEKVFDHSDHMWLILG
                     GRQNFLPQFESLLPAPVRKKIVGHIVIDPEASLNEVLGKAYTIAEEAEAAHEKEIIEQ
                     LRGEASNGGKGIFGLQPTLQSLRKGGVSTLVVTKGYQAPGFVCHKCFFIGTPEEKGKH
                     DTCPICSGPAHNVEDVVEEAITFAYMQGCKVETTNENSRMRIMGDFGALLRY"
     gene            5895..7175
                     /locus_tag="PK754_08730"
     CDS             5895..7175
                     /locus_tag="PK754_08730"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HNB09629.1"
                     /translation="MTTTCSVFLTIFVLCFAQAQSSNGQNLDHIEKFNSVKSLFTSRC
                     ISCHGGDEPAAGLRLDRNLYAQIVNVPSNGNPDLHLIQPNSPESSYLYRKVARQPNEL
                     PFADDPMPLDDIPLSSSELQIIREWILSFDPALWPAENQNNTIIQNPATEIVPKFLAT
                     QLINMPSTQTLGNRSAEFRIMHRFGLINGGPDGGNTYGGFFGMDAGAITSINFSIALT
                     NNADILLRRTGTNKDFELGVKYVPFHFNERFPTDVGFFGSIDWISRDDVPARHKISPN
                     LQMMIGSRPFENLSILIAPGIAFKSNHNATITKTVNDTTTQAYRDTRATTAIGLGAEY
                     LIGRGYAISGEYIPRLGGYKGNSFSNDRRYDNWSLAFSYKVRLHVFQLLVSNTQTMHT
                     TQYLPGSSTITKNALYNKGWNFHFGFNIYRQFKW"
     gene            7169..7576
                     /locus_tag="PK754_08735"
     CDS             7169..7576
                     /locus_tag="PK754_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09630.1"
                     /translation="MVKEIKLFSLLVFVMILLSACGELKEESQPTLLVPADVQAIFND
                     QCTGCHSGSSPAGSLNLSVGNAYGQLVNVTSTIEPALKRVVPSDTANSYLVDKIMGTQ
                     TVGERMPDGGPYLSAAQMNIIKLWIQAGAEAKP"
     gene            complement(7621..>8789)
                     /locus_tag="PK754_08740"
     CDS             complement(7621..>8789)
                     /locus_tag="PK754_08740"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNB09631.1"
                     /translation="RIEGNYWNGILCDIMSTPKINNNQIQNNGYAGINAKINSAPSIN
                     ANEITGNRYGVLVQDVSKPLIGDTRLGENSGKNAIYNNLEFNLYNHTKNVVYAQRNDW
                     GDNFNADRFIHDDDENNKFGLVVFTPVYSTGNISYLEFKNIAADAPTDTKAEQEKRRK
                     EIEDLKRQLAQAKPTDKAGGAGLTESERQKRAEDARKEKERLALLEEQLKQQEQIRIE
                     QERRLALQKQQEEDQKKTAAAATVKQPVVTVNKETAAATTTPTPEASTTNAASAGTTP
                     KPVESKPAAPVFVPTKMANELDNNPKALQKVSPVMPDLAKKAKLNGTVSMRVLVGTDG
                     KPEEIYVSKRIGNKDWDQMINDAAIAAVKQWLFEQGLSGGVPVKYWTVVTVLMK"
BASE COUNT         2427 a         2068 c         1862 g         2432 t
ORIGIN      
        1 aaaaaagact catcaaccaa tcctaaagaa gcctgccgcc aaagtggtgt ccagcaagtc
       61 gccgcaggtt atattattta cggttccagt acgatgtttg tttacacggc cggtcatggt
      121 gtacacggtt tcacactgga cccggggctc ggcgaattta tactctcgca tgaaaatata
      181 aaaacacccg aacgcggaaa aatatacagc atcaatgaag gcaattcgaa caagtggcac
      241 gacggtattc ggaattacat cgcatatctt aaagaagaaa ataaatccga taaacgtccc
      301 tattcctcgc gttatatcgg atctttggtt gccgattttc atcgcaattt actctatggt
      361 ggtattttcc tttatccggc ggatcgttcc aatcctgagg gaaaattgcg tttattatac
      421 gaagccaatc ctttagccat gatcgttgaa gcagccggag gagccgcttc cgacgggcat
      481 cggcgcatca tggaaatcga acccacggct ttacatcaac gtgttcctct tatcattgga
      541 agtaaagcgg acgtagaaac agcgtgtgct tttattcaag gtcagcgtta atcgtcaatt
      601 ctgttttatg atgattggat taaaggcctt ggaaaccacg gtaacgcgat caccgatttg
      661 aagtgattcg atatccgaag ccgttgctac aacccgaatc aatcgctccc ctgatgcgat
      721 cgtgagaata aatatcgtat cattgggctc catgcctacg acttcaccat ccattctaaa
      781 ctttccgctc aaatgtttat cgtgataaac atccatcgga ttacccagct tgaccattcg
      841 cccattatcc aaaacgcaaa cccgatttga taaacggcaa atttccgcca tgtcatggct
      901 tacccaaacc atcatcgaag gcgcttcatt ccacagagtt aatatctctt gctgcatttt
      961 ttttcgcaac gccacatcca ttgcggcaag cggttcgtcc atcaggagaa gcaccggctt
     1021 acgaatcaat gctcgcgcca aagcaacacg ttgcttttga ccgcccgaca acgaatcagg
     1081 tcttcgcgaa gcaagaccct ccagttcggt gagttgtaac attcgccgga ccgcttcatc
     1141 atcgggttca ctcaacgcaa atgcgaggtt ttcatatacc gtcatattgg gaaacaatgc
     1201 ataatcctga aatacaaatc ctacggcgcg tcgttcgggg ggacgattga tttttttttc
     1261 ggaatcaaac cacggttgat cctgccaata aatgcgaccg ctatccggtt cagtgagtcc
     1321 ggccagcata cgaagaaggg ttgtttttcc ggagccggac acgccggtta atgctaaacg
     1381 ttcatgcgcg ggaatctcca tacatatttc cataacgaca tgcccggagg atgtttcaat
     1441 cgttttttta atatcaatgg atagcattat cggcctctgg cattattttt ggactcgata
     1501 acatgaatgc cgaataacac acaccacgaa aaaattacta acacaagagc atatgtgtga
     1561 gccatcgaat aattcatctg ctcgacggct tcgaatagcg cgatagaagc cacttttgtt
     1621 ttttcaggaa tacttccgcc aatcatgagt acaacgccaa attcgccaac cgtatgcgca
     1681 aacgtcatga tagacgccga aacgacagcc ggacgcatat tgggtaatag aacacgatat
     1741 agtatctccc ctcttgattt tcccaatgtt tgtgccgctt cgatcaacga tcggtgcact
     1801 gaaccaaaac ccgtacgcaa cggattaacc ataaacggta aactgaaaat aaacgaaccg
     1861 ataagaagtc ccgtaaatga aaaggcaagg cgcacatcaa accatgattc gatcgcagcg
     1921 ccaaaccaaa atttcggtga aaacgcgatc agcaaataaa atcctaatac cgatggaggc
     1981 aataccagag gcatgcttac caacgtttgc aacggtatgc gccatttgct tttagtaaat
     2041 gcaagatagt aagccaacgg aataccggct atcagcaata ataccgtcgt acaacctgcc
     2101 aatttgaacg tcaaccagaa aggcgcccaa tccatcatgc tacgcgtcct cccaaacagc
     2161 aaccgcttgc gcattcacgt atacttcaag attatgattt tcctccaacg acatacgctt
     2221 ggcgttttct gtagtgagaa ccgatcggat agtacctatc gcagtttgta ctcctacttc
     2281 tgtcaaaagt ttaccccatc gaatttgaac aattgttcct gtccattgat tggacatact
     2341 accgtgccgg cagtcttgtg gtaataaatt cacttcggtt tctttaaacg cagcatacac
     2401 ttgcacaccc ggttttagat acgatgccgt ctcaggtgtt tcgataataa cggctgttaa
     2461 cggagcgttc gcgctttgca tcgtcaccaa agatagtgat gcttcggttt caatggcaac
     2521 gatatggcat ttaattttat tcatgaattc ttgtataacc aaatttctca aaaacttctt
     2581 tggctgtatc cgaataaata aactcaaagc attgccgggc ctccgactta tcatttaata
     2641 aagcagcgct ttgtatgatc ggattataca gcttcgaatc aacgacacga taatatcctt
     2701 tcaaactcgt cggtgcactc aatactgatg acaacgctgt caaccctata tccgccgttc
     2761 cggttgtaat gtactgattc acttgggata tattttcacc gaaaatcagt ttatccttca
     2821 cgcgttcgta ctgaccggat tttttcatcg cgtccatggc ggccttaccg tatggcgcca
     2881 atgccgggtc agcgatagca atatgccgaa tttcggcttg gtctatagaa cccatcgcat
     2941 cgtcggagta aagcggccta gcagaccaag caaccaattg accttgggca tataaacgcg
     3001 ggcgcatacg cgtcaacttc ttttcgtata aaatattggg aaatatcgta tcagcggata
     3061 aaaaaatatc atacggcgcg ccgtgttcga tctgagtcgc aagtttaccc gatgcgccgg
     3121 aaatgatctc tacgcgaatt ccggtttgtt gcgtgaatat cgccatcaaa gtcttagttg
     3181 cgggacttac attgctagcg acggcaatac gtaccgacga ctgtgaatgt aattcgaaga
     3241 cattcaatac catcaacata cacaaattgg caaagagatt tttgatcaaa tgctgtaacg
     3301 aagtaggcat tcgttaatta ctttttaaaa tcattgatga tgataacgct cattaaggct
     3361 aatctaaaat tacaaattta cgagattaat cacaaccata gcttatttat cctcgcagct
     3421 caagttattg aaattttaac gcttatgatg ttccctcgaa aatgaaattg taaaaattta
     3481 tgtcattata cccgtgaata gtcggtttcc cttgcgaaca ctaaaagtgc gtcgtatatt
     3541 taaatgaaga cattacttca cacgcatgac cacttcgcct attttaatac aaaaacgctt
     3601 tcaatttcct ccgggtcaaa atcccttcat aaccgaagaa caacgttttt tttcatacaa
     3661 tcattacatt cgttcgcgtt tcggtcaaag gattcatcgt gttacggtgg atgccggatt
     3721 tacatgcccc aatcgcgacg gaacggtggc ttccggcgga tgtacatatt gtaacaacga
     3781 cggtttcagc ccggcttcgc agatttatcg tgtaaaaaaa taccttaaac cttcaacgcc
     3841 ggtacacgat cagataacca accatattga tgcaatgaaa aagcgtttta aaacggataa
     3901 gtttttcgcg tattttcagg cctatagcaa tacgtatgcg ccggtggacg aactcgaaac
     3961 gctttaccga gaagcccttc atcatccgga tattatcggt cttgtcgtcg ggacacgtcc
     4021 cgattgtgtg gacgaagaaa aactccagct tttacagtcc atagccgaaa ctcattacgt
     4081 cagtatcgaa tacggttgtg aatccatata cgataaaaca ttacaatggg taaatcgcgg
     4141 acatgatttt gcctgcttta tagacgcggt cgaaatgagc gcgggacgta atttggatgt
     4201 ggccgcacat attattctgg gttttccgac cgaaacacgg gacgagatgc ttgcgatggc
     4261 cggtgcaata aatcgcttac cggtagattt tataaagatt cacaacctcc acattgttgc
     4321 ccaaacaccg ctcgcctttc aatacaaaaa gtcacctttc cctgtgttcg agcaacatga
     4381 atacagcatg ttagtatgtg attttcttga aagattgtct cctaacattt gcattcaaag
     4441 gttatatggc gatgccccgc gcgctatgca aatcgctccc cggtggtcca caaccggcca
     4501 ggatatgaat ctttgggttc aggccgagtt gacacgtcgt cagtcgtttc agggtattcg
     4561 tgcaactgaa tgatcgtctg aattatttgt tctttgctac gctacttacc actgcgttac
     4621 ggtatactct gtaacgccaa aaccgtagga ggtctccatg ataagccaaa aaacgatcga
     4681 acggttatct aaaatcggca gcagagattt ttttatcacc agtttatatc tcaatttgga
     4741 tcatacacag aaacacggcc aatacaaaat cgctctgaaa gatctgctga aagaccgtcg
     4801 ccatcgtctg gatgaatttc gtaagcataa caaaataact aaggaacaga tcaaatcgct
     4861 cgaagatgat tttgttcgaa ttgaaaatta tatccagcac gaatttgtca gcaaagattc
     4921 cggaaaaggg ttggccattt tcagctgcag cgccacgcat ttctgggaaa ttctcgaatt
     4981 gcctcaaccg gttcccaatc acctcaatgc cgattttgat cccaatatcc gcattttgag
     5041 cgaattatta tgcgattacc gtcactatgc catcgctctc gttgatagca gcaaagctaa
     5101 aatcgttgac gtcaatctgg gtttcgttcg tgaagtatgg tctgtcgaac atgcgataac
     5161 aggcaaaacc aaatttggcg gcgtggatgg cacccaagag cgcaatgccg agcgagcgca
     5221 cgatgaatcc gttcataaac actttaaggt tgtggcgacc gaggttgaaa aagtcttcga
     5281 ccacagcgac catatgtggc ttatcctcgg cgggcgacaa aattttctac cgcagttcga
     5341 gtcccttctc ccagccccgg ttcgtaaaaa aattgtcggc cacatcgtga ttgatccgga
     5401 ggcatcgctc aacgaagttt tgggcaaagc gtacactatc gccgaagaag ccgaagccgc
     5461 tcatgaaaaa gaaattattg aacaattgcg cggcgaagcc agtaacggag gtaaaggaat
     5521 ttttggctta caacctactc tgcaaagcct ccgcaaaggc ggggtaagca cgcttgtagt
     5581 tacgaaagga tatcaggcgc cggggttcgt ctgtcataaa tgttttttta taggcacacc
     5641 tgaggaaaaa ggcaaacacg atacttgccc gatctgttcc ggaccggcac acaatgtcga
     5701 agatgtcgtc gaagaagcca ttacttttgc ttacatgcaa ggatgtaaag tagaaaccac
     5761 caatgaaaat tcgcgcatgc gtatcatggg tgactttgga gcgctactta ggtattgacc
     5821 tcatgatgag tttgttatat gttacaaaag gtaagctaaa cggcttacct tttttttaac
     5881 aaacacgata tactatgacc accacatgct ctgtatttct tacgatcttt gtcctgtgtt
     5941 tcgcgcaagc ccaaagtagc aacggtcaaa atttagatca catcgaaaaa ttcaacagcg
     6001 taaaatccct atttacatcg cgatgcattt cttgccacgg tggtgatgaa ccggcggccg
     6061 gtctgcggct tgatcgaaat ctctatgcgc agatcgtcaa tgttcccagt aacggcaatc
     6121 ccgacttaca tctcattcag cccaactctc cggaatccag ttacctctat cgaaaagtag
     6181 caagacaacc aaacgaatta ccttttgcgg atgatcccat gccgctggac gatattccgt
     6241 taagttcttc cgaactgcaa atcatacgtg aatggatcct ctcatttgat ccggccttat
     6301 ggcctgcgga aaatcaaaat aacacgatta ttcaaaaccc tgctacagag atcgtaccaa
     6361 agtttctggc tacacaattg atcaatatgc cttcgacgca aactttgggt aaccgtagtg
     6421 cagaatttcg catcatgcac cgttttggct tgatcaacgg cggtcccgac ggtggaaata
     6481 cgtatggagg cttttttggc atggatgcgg gtgctataac gagtatcaat ttttccatag
     6541 ctttgacaaa taatgcggat attctgcttc gacgtacagg aaccaataaa gattttgaat
     6601 tgggcgtcaa atacgtaccg tttcatttta acgaacgttt tccgacggat gtcggttttt
     6661 ttggaagcat agactggatt agccgagatg acgtgcccgc gcgtcataaa atttcaccta
     6721 atctccaaat gatgatcggt agccgcccgt ttgagaacct gagcatactc atagcgcccg
     6781 gaatcgcgtt taaaagtaac cacaatgcta caatcaccaa gacggtcaac gatactacaa
     6841 cccaagccta tcgcgataca cgggctacta cggctatagg actgggggcg gaatatctga
     6901 taggccgagg ctatgcgatc tcaggtgaat acattcctcg tctcggcggc tataaaggaa
     6961 attcgttttc caacgatcgc aggtatgata attggagcct tgctttttcc tacaaagttc
     7021 gcctccacgt atttcagctt ttagtttcca atacccaaac gatgcatacc actcaatatt
     7081 tgcccggaag ttcaactatt accaaaaatg cattatacaa taagggttgg aattttcact
     7141 tcgggtttaa catatacagg caattcaaat ggtaaaagaa atcaaattat tttcgttact
     7201 cgtttttgta atgattttat tatcggcatg cggcgaattg aaagaggaat ctcagccgac
     7261 gttattagta ccggcagatg tgcaggctat atttaacgat caatgtaccg gttgccactc
     7321 tggctcgtcc cctgcaggat cgcttaattt gtccgtgggt aacgcgtatg gccaattggt
     7381 gaatgtcaca agtactattg agcctgcatt aaagcgcgtc gtaccgtcgg atacagcaaa
     7441 tagctatctt gtggataaaa ttatgggcac acaaacagtt ggagaacgta tgccggatgg
     7501 cgggccctat ttgtcggcag ctcaaatgaa cattatcaaa ttgtggattc aagccggtgc
     7561 ggaagccaaa ccttaattgc atcagtcggc tgtaggcgaa aatcaaaaaa ataattctgt
     7621 ttatttcatt agaactgtta ccacggtcca atatttgacc ggtacgccac cggataatcc
     7681 ctgctcgaat aaccattgct tcacagcagc gatcgcggcg tcgttgatca tttggtccca
     7741 gtctttgtta ccaatgcgtt tggaaacgta aatttcttca ggttttccat ctgttcccac
     7801 aagtacgcgc atcgaaacag ttccattcag cttagccttt ttagcaagat caggcatgac
     7861 cgggctgact ttttgaagcg ctttaggatt attatctaac tcattggcca tctttgtggg
     7921 tacaaaaacc ggtgctgcag gtttagattc tacaggcttg ggcgtggttc ctgcggaagc
     7981 ggcgttagtc gtggacgcct ccggagtagg ggtcgttgtg gctgcagcgg tttctttatt
     8041 tacggtcacg acaggctgtt ttacggtagc cgcagccgcc gttttctttt gatcttcttc
     8101 ctgctgcttt tgtaatgcaa gtcggcgttc ttgctcaata cgaatctgct cctgctgttt
     8161 gagctgttct tcaagtaaag ctaaacgctc tttttcctta cgtgcatctt cggcgcgttt
     8221 ttgccgctcc gattcggtta accctgcccc accggctttg tcggtcggtt tggcctgcgc
     8281 caattgtcgt tttaaatctt cgatttcttt gcgacgtttt tcttgctctg ctttagtatc
     8341 cgtcggcgca tcggccgcga tgtttttaaa ttccagataa gatatgttac ctgtgctgta
     8401 caccggggta aatacgacga gaccgaattt gttattctca tcatcgtcat ggataaagcg
     8461 atcggcgtta aaattatcac cccagtcgtt acgttgtgcg taaacgacat ttttggtatg
     8521 gttgtataga ttaaactcca aattattata aatagcattt ttcccgctat tttcgccaag
     8581 gcgcgtatcg ccgatcagcg gtttagagac gtcctgtact aaaacaccat aacgattgcc
     8641 cgtaatttca ttggcgttaa tgcttggcgc tgaatttatt ttggcattga taccggcgta
     8701 accgttattc tgtatctgat tgttgttaat tttaggtgtg gacataatat cgcaaaggat
     8761 accattccaa taatttcctt ctatgcggt
//
LOCUS       DAOQNQ010000123         8739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_2979_length_8739_cov_5.406380, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000123 DAOQNQ010000000
VERSION     DAOQNQ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8739
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2979_length_8739_cov_5.406380"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PK754_08745"
     CDS             complement(<1..549)
                     /locus_tag="PK754_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNB09632.1"
                     /translation="MSRLRTLLAEKFPDLQHKTLIAEMEKHSRLMTANPGDMLVKTND
                     YVKVVPLVTKGSIKVSRDDGEGHALFLYHIEPGQSCAMSLKSCLGEERSRIKGEADSV
                     TELIAIPSSFIEPWMTEFPAFKRFVINTFSQRYDELLSMIDTIAFEKMDRRIMTCLRQ
                     HARAGDGSTVRLTHQKVADELNT"
     gene            complement(591..1022)
                     /locus_tag="PK754_08750"
     CDS             complement(591..1022)
                     /locus_tag="PK754_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HNB09633.1"
                     /translation="MPYDERLVAPMRAELTKIGFTELKTKAAVDEALQNQGTTLVVVN
                     SVCGCAAGMARPGVAMSLNNAKRPDHLTTVFAGQDTEAVARVREHIKEFPPSSPSAAL
                     FRNGKLVWMLQRFEIEGQTAMAVANKLAAAYEKHCVPATQG"
     gene            complement(1190..1480)
                     /locus_tag="PK754_08755"
     CDS             complement(1190..1480)
                     /locus_tag="PK754_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09634.1"
                     /translation="MCAANSTDVHIPLKGCRLDRYLCIKTERAVAKDYTIAHHGKLYQ
                     LHTRLPLKRVVVEERCDGTMYVVSGDHFVKHTEITHRPMKNFSIYNQPSGWL"
     gene            complement(1582..2262)
                     /locus_tag="PK754_08760"
     CDS             complement(1582..2262)
                     /locus_tag="PK754_08760"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HNB09635.1"
                     /translation="MSDRQVRRLAKAVRSRGEMGIVHGLRGKRSNRKIPEGVKAKVLS
                     QYQKRYLGFGPTLACEKLLEVDHLVVSDETLRPWLMEAGLHEKQRRTRVHRSWRRRRE
                     CLGEMIQIDGSHHAWLEDQGEELVLMGYIDDATGEVFGRFYDYEGTLPAMDSFKRYVQ
                     QYGLPMSVYLDRHTTYKSPKKLPEGVEDQEQPLSQFECALKELGVEVIHAYSPQAKGR
                     VERLFGVL"
     gene            complement(2301..2609)
                     /locus_tag="PK754_08765"
     CDS             complement(2301..2609)
                     /locus_tag="PK754_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09636.1"
                     /translation="MDAQRFGRNNWRLMGGVDYRASSYYVQSEAVHTKSRYDRIGGRL
                     SYFGELQPYNHTELYGKIGIVNMPGASQRYWMMYVSLNYRLNPLDVIAMTKKIILPGQ
                     "
     gene            complement(2691..4565)
                     /locus_tag="PK754_08770"
     CDS             complement(2691..4565)
                     /locus_tag="PK754_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09637.1"
                     /translation="MRSIRPLLYLWMGSIGTILLGQNLQLGDIKKSVTAITENINTAD
                     QQFRMLDTAIDNLDYKACRNTLTSLRQTAWGEAPSSSLKSNIADLTRHTETAYQELAR
                     HALRDVVTKTDWIQLKEDYAEIRDSVKMIITKLRDMQQKIAGIGLTAQAKIKEKKELE
                     EKALEFITALVMKRTVSTRDSLYQVLVAQLERLGLTIEDVRFLSRWDQLEKNARYDSL
                     IVLTGKLADHWLRQETAEVFAEVLRMASDLQDKKITLDEVYTLANNRMIEKLEEEIGG
                     TALAKAAADEFSVPKVEALQVTLNHPEEVFLQPISGEQRYILYKEKIYRYEKQKTEYT
                     YIQAFDAKLENFELRFQPVIVKVRINGDPQPVADTLYINTVYLKFYHYVDQLADKLTS
                     YLSPIVTGKTSEEKVNYVDEQNNLVFEPDIRQTRSRFIGIAQDTTNGKISAYTDQIEA
                     ALYLKNLFRVELIADDYESNRLCDEKWTMIRHQPVRIKAYGPASWMIATIEPSKNIGG
                     DGQVTRVNSRNTAQIKVNFEGTLCDNRFGDKGGKFLFFISHGPMRWLRYLKAGFVLNW
                     KGDLERIDSLRGATVKEKLIDLYSENSESMLYFTFPVLSQLSRGRSYVMDWLVIRPMV
                     "
     gene            complement(4907..5158)
                     /locus_tag="PK754_08775"
     CDS             complement(4907..5158)
                     /locus_tag="PK754_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HNB09638.1"
                     /translation="MKQFPSPASEPTNKAAYETQRTRFITGMVCTRAGVYIFESYADH
                     ATDSKPTDDELYVVLKYGQVFPPIRSVCKSAWWKLEKEK"
     gene            complement(5336..5902)
                     /locus_tag="PK754_08780"
     CDS             complement(5336..5902)
                     /locus_tag="PK754_08780"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNB09639.1"
                     /translation="MLTNEQEKTLFGIPLRLDDIYPSVPPPINPPYELDEIMLTFGDI
                     FNYVNQDGSIKRSWEEGQMGFADIPFTIPFSSYPDIKVGKIYCHKKLISTFTKVFRVI
                     YEKNLHRHIHSYGGCYVYRNKNNQTQLSTHCWGIAIDLNPHTNAPGTRGDMNSDIVRV
                     FQSHGFEWGGNWKKKYRNPMHFQWCSGY"
     gene            6075..6689
                     /locus_tag="PK754_08785"
     CDS             6075..6689
                     /locus_tag="PK754_08785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB09640.1"
                     /translation="MLFAKTRYKDLSDFELIEAYRKSMNDRYLNELFSRYAYLVLFVC
                     NKYFSNPEDSRDATMEVLEKLAQGIVKSDVKNFKSWLYVVTKNLCLNKLQKSKDLDFQ
                     FVPIDGDETNYFMYFSEIDSLIDEDDDHSLKMKQALKEAIETLDPHQRECLMLFYYEK
                     KSYNEIMEMKNWDKDQVRSYLQNGRRNIKIYLKKSGLYVENIEN"
     gene            6667..7458
                     /locus_tag="PK754_08790"
     CDS             6667..7458
                     /locus_tag="PK754_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09641.1"
                     /translation="MSKISKTESESGGLKLTRSHQHCLTEEMIVNYLFRSLSDEERHR
                     IEEHITDCDLCYDAVSGSRRLGSERELTKQTMILRSEIEARYGRTHRSNKPAKFKYYA
                     AAALLLISSSSIGYWLTRHSHETIADAFLKPYSNTIPLTRGTDAQSLLEEAMAAYEAE
                     TYDASAQKLEDFLKTNPDHSVAHFYCGISLLLEGQFEESDVHFQKSEKSEDRRIAEAA
                     RWYRALAYVKMEKTDEAMNLLKEVAQRNGYYAPSAASLMERLQKE"
     gene            7455..8240
                     /locus_tag="PK754_08795"
     CDS             7455..8240
                     /locus_tag="PK754_08795"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNB09642.1"
                     /translation="MRILRVYILASAVLLGTLFAQEEEEKSARDTLRLRDLPWAEKEL
                     QSIASVVKSELYLRDDATEAAFKKNAGRFGIIHLAQHVILDDKEPLYTKFIFSDDPGS
                     GEDGYLHLYELYNLRLRANMVVLSACQTGIGKNIKGEGMMSLARGFTYSGCPSVVMSL
                     WSIDDKATSVIITDFYKKLAQGKTKDVALRDAKLNMIQSGDPVLSNPYYWSGLVVIGS
                     TESLEIPPPENFISYRIYATVVIVSILVLLILIGKKRMKSVSH"
     gene            complement(8237..8452)
                     /locus_tag="PK754_08800"
     CDS             complement(8237..8452)
                     /locus_tag="PK754_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09643.1"
                     /translation="MKKTKLAIVMYVILLTLAVSACGGPEGVVSSSTGKNSESTTGDK
                     KTYDDGSDDVFPQDTPIDPLPGGGGGN"
BASE COUNT         2341 a         2077 c         1893 g         2428 t
ORIGIN      
        1 ggtattaagt tcatccgcta ctttctggtg ggtcaaacgc accgtgctac cgtctccggc
       61 acgtgcatgt tggcgaaggc acgtcataat gcggcgatcc attttttcga aagcaatcgt
      121 atcaatcatg gataacaatt cgtcataccg ctgactaaaa gtattgatga caaaccgttt
      181 gaatgcggga aactccgtca tccagggttc aataaacgac gaagggatag caatcaattc
      241 cgtcacagaa tcggcttcgc ctttgatacg actgcgctct tcgccgagac aacttttgag
      301 agacatcgcg caggattgac cgggctcaat gtggtataaa aaaagtgcat gaccttcgcc
      361 gtcatcacga ctcaccttga tcgaaccttt ggtcactaac gggacgactt tgacatagtc
      421 gttagttttt acgagcatgt ccccgggatt ggccgtcatc aagcggctat gcttttccat
      481 ctctgcgatc agcgttttat gttgcaaatc cggaaatttt tcagcaagca atgtgcgtag
      541 ccgactcata acataatact accggtttat tatcatttta aggattcgac tcacccttgg
      601 gtagcaggaa cgcaatgctt ttcataagct gcggcgagtt tattggcgac agccattgcc
      661 gtttgtcctt cgatctcaaa tcgttgaagc atccatacta attttccgtt cctaaaaagc
      721 gcggccgacg gcgatgaagg cggaaattct ttgatatgct cacgtacccg tgcgacggcc
      781 tcggtatcct gacctgcgaa aacggtggtt aaatgatcgg ggcgctttgc attattcagc
      841 gacatcgcga caccggggcg agccattcct gccgcacatc cgcaaaccga attgacaaca
      901 accaatgttg tgccctgatt ttgcagcgct tcgtcaacgg ccgccttcgt tttcaattcc
      961 gtaaaaccga ttttggtcaa ttccgcacgc atgggtgcaa caaggcgttc atcgtaaggc
     1021 atacgttctc ctattatttc atttgtaaag tttcaaaatt tggatttaat gctatggacg
     1081 cgttactttc aatttatttt aataatattt catttcactg catttgtcaa taaggtataa
     1141 ttatatgtca agccaaaata gaaatgtccg gtttttgatt gataaatggt tacaaccaac
     1201 ccgaaggttg gttgtaaatc gagaaatttt tcataggccg atgcgtgatt tctgtatgct
     1261 tcacaaaatg atctccgctc accacataca tagttccatc gcatcgttct tccactacaa
     1321 cccgtttgag cggaagccgc gtgtgaagct gatataactt gccgtggtgc gctatcgtat
     1381 agtccttggc aacggcccgt tcggttttga tgcaaagata acgatccaga cggcaccctt
     1441 ttagcgggat gtgtacatcc gtggagttag ccgcacacac cccaaattgt ttattgtagc
     1501 gcggccaata ggtctctaaa aacgcattcg ctccttcttt gtcgtcattc cggctagacg
     1561 catttccttt accagacgat cctacagtac accaaataat cgttcaacgc gacccttggc
     1621 ttgcggagaa taggcatgaa tgacctccac acccaattcc ttcagagcgc actcgaattg
     1681 actaagcggc tgttcctgat cttcaacacc ttccgggagt ttcttgggag acttgtaggt
     1741 cgtgtgtcga tccagataca cactcatcgg taacccgtat tgctgtacat aacgcttaaa
     1801 actgtccatg gcgggcaagg tgccttcata atcataaaac cgcccaaaca cctcgcctgt
     1861 cgcatcgtca atgtaaccca ttaggaccaa ttcctcgcct tgatcctcca gccaggcatg
     1921 atgcgaacca tcaatctgga tcatttctcc taaacattcc ctgcgtcgac gccatgatct
     1981 gtgcacacga gtgcgtcgtt gcttttcatg tagtcccgct tccattaacc atggccggag
     2041 cgtttcatca ctgactacca agtgatcaac ctccagtagc ttctcacacg ccagcgtcgg
     2101 accaaaaccc aaataccgct tctggtattg gctcagcacc ttggccttaa ctccttcggg
     2161 aatctttcga ttcgaccgct taccacgcaa tccatgtact attcccatct ccccgcgcga
     2221 tctcaccgct ttcgcaagac gtctgacttg gcgatcactc aaagcaagca tcgcaccgct
     2281 tataacttcc catagtatcg ttattgtccc ggcaaaatga tcttcttcgt catcgcgatc
     2341 acatcgagcg gattgaggcg gtagttgagc gaaacgtaca tcatccagta cctttgggaa
     2401 gcgccgggca tattgacgat acctatcttg ccgtacagct ctgtatgatt atacggctgg
     2461 agttcaccaa aataactcag ccgaccgccg atcctgtcat atcgcgactt ggtgtgaacc
     2521 gcttccgact gaacatagta ggatgatgcc cggtagtcca caccgcccat gagtcgccag
     2581 ttattacggc caaagcgttg tgcatccaaa aggcgtactt gtaatccact cgacagctta
     2641 tcgtacgcac ggtatgtgcc ggcagcatcc tgtactttaa atcgttcgtg ttacaccatc
     2701 ggacggatca ccagccaatc catcacatag gaacgtccgc gcgaaagctg actgagaacc
     2761 ggaaaggtaa agtacagcat ggactcggaa ttttcggaat agaggtcaat gagtttttct
     2821 ttgaccgtcg ccccgcgtag cgaatcaatc cgctccagat cgcctttcca gttgagcaca
     2881 aatccggctt tgagataacg taaccatcgc atgggcccgt gcgatataaa aaacaagaac
     2941 ttcccgcctt tatcgccaaa gcgattatcg caaagtgtac cttcaaaatt gactttgatc
     3001 tgtgccgtat tgcgcgaatt gacgcgcgta acttgtccgt caccaccgat gtttttggac
     3061 ggttcgatgg tagcgatcat ccatgaagcc ggcccgtacg ctttgatccg taccggctga
     3121 tggcgaatca tagtccattt ttcatcgcat aagcggttgc tttcatagtc atcagcgatg
     3181 agttcgaccc gaaaaaggtt tttgagatac aatgcggctt cgatttgatc tgtataagca
     3241 gatatttttc cattggtggt atcctgtgcg ataccgataa aacgacttcg tgtttgccga
     3301 atatccggct caaaaactaa gttattctgt tcatcaacgt aattaacttt ttcttcagaa
     3361 gttttacccg tcactatggg gctgagatag gacgttagct tgtcggccag ctgatccaca
     3421 taatgataaa acttgagata tacggtattg atatatagcg tatcggctac aggttgtgga
     3481 tcgccattaa ttctaacctt cacgatcacc ggctgaaatc gcagttcaaa gttttccagt
     3541 ttggcatcaa aggcttgtat gtacgtgtac tcggtttttt gtttttcata ccgataaatt
     3601 ttttctttat ataagatata acgttgttct cctgatatgg gttgtaaaaa cacttcctcg
     3661 ggatgattga gcgtgacctg cagagcctcc actttcggta cggaaaactc gtcggccgcg
     3721 gctttggcaa gcgccgtacc gccgatctct tcttccaatt tttcgatcat gcggttattg
     3781 gccaacgtat agacttcgtc caaagtaatt tttttatcct gaagatcaga cgccatacgc
     3841 aggacttcgg caaaaacttc cgccgtttcc tgccgcagcc aatgatccgc aagtttgccc
     3901 gtaagcacga tcagcgaatc ataacgcgca tttttttcca attgatccca gcgtgacaaa
     3961 aatcgtacat cctcgatcgt caagccgagc ctctcaagtt gtgccactaa tacttgatat
     4021 aatgaatccc gcgttgatac ggtgcgcttc ataacaagcg ccgttataaa ttccaacgct
     4081 ttttcctcaa gttccttttt ttcttttatt ttggcctgag cggtcaggcc gataccggcg
     4141 attttttgtt gcatatcgcg cagtttggtg atgatcattt ttaccgaatc gcggatctcc
     4201 gcataatcct ccttgagttg tatccaatcc gttttggtta ccacatcgcg cagagcgtgg
     4261 cgtgccagtt cctgatatgc cgtttcggta tggcgcgtca gatcggcgat attgcttttg
     4321 agcgatgagg atggcgcctc gccccatgcc gtttgacgca gcgatgtgag tgtattgcgg
     4381 caagccttat aatctaaatt gtcaatagct gtatcaagca tacgaaattg ttgatccgcc
     4441 gtattgatgt tttccgtgat cgctgtgacg ctttttttga tgtctcccag ttggagattt
     4501 tgaccaagga ggatggtacc gatagacccc atccaaaggt aaagcagagg acggatacta
     4561 cgcatacgat ggccctttct atatgtatgt gtaatacgaa aaaccgacgg aataaacttc
     4621 agacggtggg cacacggtcg ctgtacctgt cccgaacaaa agagattcac aagaaatttt
     4681 taccggtaat gcatcgaagc atcgtaccgc tttgcatgac cgcgccaatg catccggtcg
     4741 tccggttatc ggcatccctg gaaatgaagt tgattatatt ttgtgttcga aaatttgttt
     4801 ctatttagcg atagctgaga caagcgatac caattcttcc gtttttaaat ttcacaaaac
     4861 gtatacccct ccgtttcatc acggcatgcg attaattcat cgcagtttac ttctccttct
     4921 ccagtttcca ccacgccgat ttgcacaccg aacggatggg aggaaaaacc tggccatatt
     4981 taagtacgac atacaattca tcgtctgtgg gttttgaatc ggtagcatga tcggcatagg
     5041 attcaaaaat ataaaccccg gcgcgcgtgc aaaccatacc cgtgataaaa cgcgtgcgtt
     5101 gcgtttcata tgccgccttg tttgtcggct ccgatgcggg agaaggaaat tgtttcattt
     5161 tacacaaatg ctatgtttga aggttttgtt acactttttt gaatcatgct atgatgtctc
     5221 atggacccca gcacggaggc ccggtacggc aattagaagt gggaggggtt ttcttttatt
     5281 gtgtaccggc ctcggctgat attaacgata caaagagacc caaaattcat atttgttaat
     5341 acccggagca ccattggaaa tgcatcgggt ttctatattt ttttttccaa ttgccgcccc
     5401 attcgaaacc atgcgattga aatacacgaa cgatgtcgga attcatatct cctcttgtcc
     5461 ccggcgcatt cgtatgagga ttgagatcaa tagctatacc ccagcagtgc gtggacagtt
     5521 gggtttgatt atttttatta cggtacacat aacagccgcc atacgagtga atatgccgat
     5581 gcaaattttt ctcatatatg acccggaata ctttagtaaa agttgaaatt agttttttgt
     5641 gacagtatat tttgcccact tttatatccg gataacttga gaacggaatg gtaaatggaa
     5701 tatccgcaaa tcccatctgt ccctcttccc aactgcgttt gatcgaaccg tcttgattga
     5761 cataattaaa aatatcaccg aacgtcagca tgatctcatc caattcataa ggtgggttta
     5821 tcggcggcgg cactgacggg tagatatcat ccagtcgaag cggaatccca aacagcgttt
     5881 tttcttgttc attagtgagc atcgagttct ttgccttggt tccattatag cttattgcaa
     5941 taagacagca tttttgaaaa aaatgcataa actaagtttt taaccttgat attttatcaa
     6001 acgcattaaa atcaatctct taaaatgagg ttttgcattt tgaaatacgg attttaatca
     6061 tcggaatgcc atttatgctg tttgcaaaaa caagatataa agatttatca gatttcgaac
     6121 tcatcgaagc ctatcgaaaa tctatgaacg atcggtatct taacgaacta tttagtcgtt
     6181 atgcgtatct cgttttattt gtttgtaata aatatttcag taatcccgaa gactccagag
     6241 atgctacgat ggaggttttg gagaaactgg cacaagggat cgtcaagtcc gatgtgaaga
     6301 attttaagtc atggttgtat gtggttacaa aaaatctctg tctcaataaa cttcaaaaat
     6361 ctaaagactt agacttccag tttgttccta tcgatggcga tgaaacaaat tattttatgt
     6421 atttttctga gattgacagt cttattgatg aagacgatga tcatagtttg aaaatgaagc
     6481 aggctttaaa agaagccata gaaaccttgg atccccatca gagagaatgt ctgatgctct
     6541 tctactatga gaaaaaaagc tataatgaaa tcatggaaat gaaaaattgg gataaagacc
     6601 aagtccgtag ctatttgcag aacggacgaa gaaacattaa gatctatcta aagaaaagcg
     6661 ggctgtatgt cgaaaatatc gaaaactgaa tcagaatccg ggggcttaaa attgacgcgt
     6721 agtcaccagc attgcctgac ggaagaaatg atcgtcaact atctgtttcg ctccctatcg
     6781 gacgaagagc ggcatcgcat cgaagaacat attaccgact gcgatctgtg ctatgacgcc
     6841 gtaagcggtt cgcgccgatt gggttccgaa agggaattga cgaaacagac catgatactg
     6901 cgatcggaaa tagaagcacg ttatggccga acccatagat ccaataaacc cgctaagttc
     6961 aaatattatg ccgcggcggc tttactattg atttcatcga gcagcatcgg ttactggctg
     7021 acacgccatt cccacgaaac cattgccgac gcgtttctca aaccatactc caataccata
     7081 cctttgaccc gtggcaccga cgcgcaaagt ttactggaag aggccatggc cgcttatgaa
     7141 gccgaaacgt acgacgccag cgctcagaaa cttgaggatt tcctcaaaac caatccggat
     7201 cacagcgtgg ctcactttta ttgcgggata agcctgctct tggaaggcca atttgaagaa
     7261 tccgacgtgc actttcaaaa atcggagaaa tcggaagacc ggcgcatcgc agaagcagca
     7321 cgttggtacc gggctttggc ctatgtgaaa atggaaaaaa cagatgaggc catgaacctg
     7381 ctaaaagaag tcgcccaacg taacggatat tacgctccgt cggcggcttc cctcatggaa
     7441 cgcctccaaa aagaatgaga attttaagag tatatattct tgcatccgcc gttttattgg
     7501 gaaccctctt tgctcaggag gaggaagaga aaagcgcccg ggatacgctg aggttacgcg
     7561 atctgccatg ggcggaaaaa gaattacaaa gcattgcttc cgtcgtaaaa agtgaactct
     7621 acctgcgcga cgatgccacc gaggccgctt tcaaaaaaaa tgccggacgc tttggcatta
     7681 tccatctcgc ccaacacgta atccttgatg ataaagaacc tctctatacc aaatttatct
     7741 tttctgatga ccccggatca ggggaagacg ggtatctcca cctttacgaa ttatacaacc
     7801 ttcggcttcg cgccaatatg gtcgtactga gtgcatgcca aacaggcatc ggaaaaaata
     7861 tcaaaggcga aggaatgatg agcctcgcgc gaggttttac ctacagcggt tgcccgagtg
     7921 tcgtgatgag cttatggtct attgacgata aagcgacttc cgtgatcatc actgattttt
     7981 ataaaaagct cgcgcaaggc aaaaccaaag atgtcgccct gcgcgatgct aaactaaata
     8041 tgatccaaag cggtgatccc gttttatcca atccttatta ttggtccggg ttggtcgtca
     8101 taggaagtac ggaatcacta gaaattcctc ctcccgaaaa ttttatatcg taccggattt
     8161 acgccaccgt cgtgatcgtt tcgatccttg tacttttgat tctcatcggt aaaaaaagaa
     8221 tgaaatccgt ttcgcattaa tttccgccac cgcctccagg aagcggatca atcggcgtat
     8281 cttgcgggaa aacatcatcg ctaccatcat catatgtttt cttgtcgccg gttgttgatt
     8341 ccgaattctt tcctgtactg ctgctgacca ctccttcagg cccaccgcaa gccgaaacag
     8401 ccaacgttaa taaaataacg tacatcacaa ttgccaattt agtttttttc atgacactct
     8461 ccttaggggt tattagttat gtaccaaata taaccaataa gacaatcatt cctgagaaag
     8521 tgcataaact tttacgggcg ggcaatctgg gacgagcaaa aaaatcgtgt cacttgaacg
     8581 tggtggacgt gaagccggct aaatcattaa gcccatctct taagaccgaa gacggctggt
     8641 ataataatga agcggaatac gcataactaa tgtggtattt tttgatgaga tagttgagtg
     8701 tagcatagtc ttcttgatta tcgggtaacc ggggcagta
//
LOCUS       DAOQNQ010000124         8617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3026_length_8617_cov_6.384022, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000124 DAOQNQ010000000
VERSION     DAOQNQ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8617
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3026_length_8617_cov_6.384022"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PK754_08805"
     CDS             <1..608
                     /locus_tag="PK754_08805"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF036996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HNB09644.1"
                     /translation="YPIPEVCVFFDYKLFRGNRSKKISIDEFDAFVSPNFGTLADVGL
                     HIEIHENLLRSPSDMLRFDTAFGRDVISIRLFPGMNAALLDGLAQTSAKVFILEAFGS
                     GNVPTLENSVIPFIKKTTETGKMVAIASQCISGGIDLNLYDCGKEALMAGAVSCGDMT
                     SEAAIIKAMYLLGQFDGHVGRVRSNFNTSIAGEITESDSNS"
     gene            605..1456
                     /gene="panC"
                     /locus_tag="PK754_08810"
     CDS             605..1456
                     /gene="panC"
                     /locus_tag="PK754_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNB09645.1"
                     /translation="MTIITSPSAMRAWTAQQKSENHRVGFVPTMGYLHQGHLSLVSLA
                     RTEGKCDRVVMSIFVNPTQFGPNEDFEKYPRDLERDRVMAETAGVDVLFFPSVDAMYS
                     TDATTMVDVGAMGTVMCGISRPTHFRGVTTVVAKLFSIVRPDTAVFGQKDAQQFFIIE
                     RMTRDLHMDVALIMAPIRREQDGLAMSSRNVYLNTDERHQALALNEALREAESLIRNG
                     ERNVDVIRTHMESIFARYPLVRKDYIAMVEAGSLAKREVIQGRTLIAVAAYLGKTRLI
                     DNIIITV"
     gene            1463..2203
                     /locus_tag="PK754_08815"
     CDS             1463..2203
                     /locus_tag="PK754_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNB09646.1"
                     /translation="MDSRFTTVIMAAGKGTRMKSDLAKVLHPLHGRPMIHYVIALART
                     LGSDRVIAIIGHQKERVREALADEKIEFAVQEPQLGTGHAVMQTESLLQNDHGDVLVL
                     SGDVPVLTEATMRKLIRLHQSESAVASVLTATMPDPTGYGRVLRHEDGRVRKIVEHKD
                     ASDEERRINEINSGIYMFQSQSLFAALKKITNSNAQNEYYLPDVIKIFIDAGQKVCAY
                     PTDNFREISGINTVEQLREAEAIMQPQS"
     gene            2232..3251
                     /locus_tag="PK754_08820"
     CDS             2232..3251
                     /locus_tag="PK754_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNB09647.1"
                     /translation="MYHVTLIPGDGIGPSIMEAAVEVVEATGVKFNWDTRLAGMAAVE
                     KFDSPIPFETLDSIRKNRVAFKGPLTTPVGGGYRSVNVAMRQEFELYANVRPAVSFEG
                     IETRYQNVNILMVRENTEGLYAGVEHSIKVDGKVIAAESTAIITRHESERCIRYAFEY
                     ARKNKRKRVTLVHKANILKATTGLFLEIGKEIAKEYPDVEFNDVIIDACAMKMVMDPT
                     QFDVFVTTNLFGDILSDLAAGLVGGLGLTPGANIGKSAAIFEAVHGSAPDIAGKNIAN
                     PTAVIMAGMMMLEYLGEFEAARRVETAVKNVIKEGKIVTPDIRKDTAYGTKEFGKEVA
                     RRVRG"
     gene            complement(3373..4359)
                     /locus_tag="PK754_08825"
     CDS             complement(3373..4359)
                     /locus_tag="PK754_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNB09648.1"
                     /translation="MKLVLRIVAGLFGLFILAIIGGYVYIKTALPNAGPVPQMVIASS
                     SEQIERGKYLANHVAVCTDCHSKRDYSFFAGPLKPETFGAGGEEFPESMGFPGTLYAR
                     NITPAGLKDWTDGEIFHALTTGIRKNREPIFPLMPWHRYATSDKEDILSIIAYIKTLK
                     PIENQVPETRLNFPLNLIVRTMPKAPAFVTRPDTTDEVAYGKYLANMAACADCHTMTK
                     KGEPLPGMDFAGGFQFIMPQGVLRSANITPDEKTGIGTWTREQFINRFKTYAQESAQR
                     IPVVEPYTETQFQTVMPWIMYAGMTEKDLGSIYAYLRSVKAVDHPVKKFTVN"
     gene            complement(4380..4820)
                     /locus_tag="PK754_08830"
     CDS             complement(4380..4820)
                     /locus_tag="PK754_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 family protein"
                     /protein_id="HNB09649.1"
                     /translation="MAGYSGTPLVKKIGIKEGYSVAWVDAPATLKALIEPLPEKARLT
                     RKVVADHDCVHWFTKSADSLEIELPKLKKKIKDSGMIWVSWPKKSSGVRTDITEDMIR
                     ECARRNGLVDVKVCAVDDVWSGLKLVIPVKDRTSLTKKNKSPKL"
     gene            complement(4967..5347)
                     /locus_tag="PK754_08835"
     CDS             complement(4967..5347)
                     /locus_tag="PK754_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB09650.1"
                     /translation="MMYRVGIIIFMLATACGAQDSFRSVNSDTLNQLLKDKPGVLLDV
                     RTVGEFSTGHLKDAVLLDFMKPEFESELEKLDKSATYYVYCRSGNRSAKAASLMNQKG
                     FQSVYNVADGMIGIVKTMPDQVVR"
     gene            complement(5347..5688)
                     /locus_tag="PK754_08840"
     CDS             complement(5347..5688)
                     /locus_tag="PK754_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNB09651.1"
                     /translation="MAKKLFDKEKMSEESLALIAKRFKALSEPMRLKIIQTIWHSEMS
                     VQDIVAKVQTSQVNVSKHLAVLHECGFLKRRKEGLFTFYSVADTSVFTLCDMMCSVVK
                     DKLNDQIRSFS"
     gene            5830..7233
                     /locus_tag="PK754_08845"
     CDS             5830..7233
                     /locus_tag="PK754_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019986612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB09652.1"
                     /translation="MYFQNIYEKGLAQASYLIGCQATGTAIVIDPRRDIHVYLEAAAR
                     EKFTITHVTETHIHADFLSGARELAAATGAELLLSDEGGADWQYAFPHTGLRDGSVFK
                     VGNLKFEVMHTPGHTPEHIAFVLTDVPAGDYPIMFFSGDFVFVGDIGRPDLLEKAAGV
                     TGTMERGAQQMFHSLKKFKSLPDHVQVWPGHGAGSACGKALGAVPGTTVGYEKLVNWA
                     LRYTDENEFVKELLSGQPEPPYYFAMMKKLNKEGPPILGTSSGLKRLTTDDATQAIRN
                     GQTLIDTRPKTAFAEKHIPGAINIQNNNALSTWAGWILSYDKPFLILVSENDAQDVYH
                     KLIRIGLDHCTGYITENRFWEESGGTLHSLRQIFATDLSAKLSDPNVAVIDVRGVSEY
                     ETGHIAGAVHIHGGHLSRRLADLPKNKTLIIQCQGGDRSSTATSLLLKNGFSNIYNLI
                     GGIAAWQKAGLPVVKEN"
     gene            7245..7628
                     /locus_tag="PK754_08850"
     CDS             7245..7628
                     /locus_tag="PK754_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB09653.1"
                     /translation="MPTWMLLLVTGVILWSLYKKKLGKNTMFQNLNNDAFSEKMKNAD
                     HVVLDVRTDAEYASGHLPKAKLINVMDASFSSKVDALDKSKTYLVYCRSGSRSRGACE
                     IMASRGFQVFNLDNGIMGWNGPVER"
     gene            7630..7827
                     /locus_tag="PK754_08855"
     CDS             7630..7827
                     /locus_tag="PK754_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09654.1"
                     /translation="MEYIKKHRFWIGPLAGIIAGFFYWKYIGCLSGACPLQSSLWVNL
                     LLGALIGYTIADTLRTLPEKS"
     gene            8237..>8617
                     /locus_tag="PK754_08860"
     CDS             8237..>8617
                     /locus_tag="PK754_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNB09655.1"
                     /translation="MSTQIDAWQMTTSGQPMNRVASAIPSLGPDEALIRIAACGVCHT
                     DLGFYYDGVRTVSPLPLTLGHEISGQVVDTGLSATAWKNKFVLIPAVLPCGTCEMCQA
                     GQPRICRHQKMPGNHIHGGFASHIV"
BASE COUNT         2308 a         2049 c         1965 g         2295 t
ORIGIN      
        1 cctaccctat ccccgaagta tgcgtttttt ttgattacaa actttttcgc ggtaaccgat
       61 ctaaaaaaat ttcaattgat gaattcgatg ctttcgtttc accgaacttt ggtacgttgg
      121 cggacgtagg ccttcatata gaaattcatg aaaacctttt acgttcacct tcagatatgt
      181 tgcgatttga tacggctttt ggccgagacg tgatcagtat ccgcctcttt ccgggtatga
      241 atgcagcact tttggacgga ttggctcaaa catcagcgaa agtatttatt ctcgaagcct
      301 ttggttccgg taacgtacct acgctcgaaa attccgtcat cccctttatt aaaaaaacaa
      361 ccgaaaccgg taaaatggta gccatcgcaa gtcaatgcat cagcggcggc atagatctca
      421 atctttatga ttgcggtaaa gaagcgttga tggccggggc cgtttcgtgc ggtgatatga
      481 catcggaagc cgctattatc aaagcaatgt atctgctcgg ccaatttgac ggtcatgtcg
      541 gacgtgtacg gagtaatttt aatacgtcca ttgccggtga aatcacggaa tccgattcca
      601 actcatgacg atcattacat caccgtcggc catgcgcgca tggacagctc aacaaaaatc
      661 agaaaatcac cgcgtcggtt ttgtgccgac gatgggttat ctgcaccaag ggcatttaag
      721 tttggtctcg ctggcacgca ccgaaggaaa atgcgatcgc gtggtcatga gtatttttgt
      781 caatcctacc caatttggtc ccaacgaaga ttttgaaaaa tacccgcgcg atctagaacg
      841 cgaccgggtg atggccgaaa cggccggcgt ggatgttttg ttttttccat cggtcgatgc
      901 tatgtattcc actgacgcga cgacgatggt ggatgtcggc gcgatgggta cagttatgtg
      961 cggcatttct cgccctactc attttcgcgg agtgacgacc gttgtagcaa aacttttttc
     1021 gatcgtacga cccgatacgg ccgtctttgg tcagaaagac gcacaacaat tttttatcat
     1081 tgagcgaatg acgcgtgatc tccacatgga tgtcgctctg atcatggcgc caatacgccg
     1141 tgaacaagac ggccttgcga tgagcagccg caatgtttac ctcaacacgg atgaacgcca
     1201 ccaggctttg gctctgaatg aagccttacg cgaagccgaa tccttgattc gtaacggtga
     1261 aagaaatgtg gatgttatcc gtacgcatat ggaatccatt tttgcacgtt atcctctggt
     1321 gcgaaaagat tatatcgcca tggtcgaggc ggggtcgctc gctaaacgtg aggttatcca
     1381 aggtcgtaca ttgatcgccg tggccgcgta ccttggtaaa acaagactga ttgataatat
     1441 cattattacc gtataaggag ttatggacag ccgatttaca accgttatta tggccgcggg
     1501 aaaaggcaca cgcatgaaat ccgatcttgc caaagtgttg catcccttgc acggtcggcc
     1561 gatgattcat tatgtgatcg ctttagcgcg taccttgggc tccgatcgcg tgatcgccat
     1621 catcggacat caaaaagaac gggtgcgcga agcgctcgcg gatgaaaaga tcgaatttgc
     1681 ggtgcaggag ccgcaacttg gtacgggaca tgccgtgatg caaacagaat cgttactgca
     1741 aaatgatcac ggcgatgttt tagttttgtc cggtgatgtc cctgttttga ccgaagcgac
     1801 gatgcgaaaa ctgatccgat tgcaccaatc cgaaagcgcg gtggcttccg tgctcaccgc
     1861 gaccatgcct gatccgacgg gttacggacg tgtgctccgt cacgaagatg gtcgcgttcg
     1921 taaaatcgtc gaacacaaag atgcatcgga cgaagagcgc cgcatcaacg aaatcaactc
     1981 cggtatatat atgttccaat cgcagtcgct ctttgccgcg ctcaaaaaaa tcaccaatag
     2041 taatgcacaa aacgaatact atcttccgga tgtaatcaaa atttttattg atgcagggca
     2101 aaaagtctgc gcttatccga cggataattt tcgtgaaatt tccgggatta acaccgtcga
     2161 acaactgcgt gaagccgaag cgatcatgca gccccaatct taaaacctac cataaatcag
     2221 gagaattata aatgtatcat gtgacactta ttccgggcga tggcatcggc ccgtcgatca
     2281 tggaagccgc cgtcgaagtc gtcgaagcga cgggcgttaa atttaactgg gatacgcgcc
     2341 ttgccggcat ggcggccgta gaaaaatttg attcgcccat tccgtttgaa acgctggatt
     2401 ccattcgcaa aaatcgcgtt gctttcaaag gtccgctcac cacacccgtc ggtggcggtt
     2461 atcgcagcgt caatgtggct atgcgtcagg aatttgaact ctacgccaac gttcgcccgg
     2521 cagtatcttt cgaaggcatt gaaacccgct atcagaatgt caatatcctc atggtccgtg
     2581 aaaacaccga aggtctctat gccggtgtcg agcactccat caaggtggac ggcaaagtga
     2641 ttgccgcaga aagtacggct attattaccc gtcacgaatc ggagcgttgt attcgttatg
     2701 cattcgaata cgcacgaaaa aacaaacgca agcgtgtcac gctcgttcat aaagccaaca
     2761 tcctcaaagc aacgacgggt ttatttcttg aaatcggaaa agaaatcgcc aaagaatatc
     2821 ccgacgtcga atttaatgat gtgatcattg acgcgtgcgc gatgaaaatg gtgatggatc
     2881 cgacgcaatt cgatgtgttc gtgacgacca accttttcgg tgatattctc tccgacctcg
     2941 ccgcaggcct cgtaggcgga ctgggcctga cccccggcgc caatatcgga aaaagcgccg
     3001 ccatctttga agccgtgcac ggtagcgctc cggacatcgc cggaaaaaat atcgccaatc
     3061 cgacggccgt gatcatggcc ggtatgatga tgctggaata tctgggcgaa tttgaggccg
     3121 cacgccgcgt ggaaacggcc gtcaaaaacg tgatcaaaga aggaaaaatc gtcacgccgg
     3181 acattcgcaa agatacggca tacggcacga aagaatttgg aaaagaagtg gcgcgacgcg
     3241 tgcgcgggta gcgcttatgc gttgatattt tctgctctca aaaaaaacaa accggaaaag
     3301 aataaacctt ttccggtttt ttgtttcgcc gtctcagcga tcaatcaatt tttagaaacg
     3361 attgcccctt cgttagttca ccgtaaattt cttcacggga tgatcaaccg ctttgactga
     3421 gcgaagatat gcataaattg aacccaaatc tttttccgtc atccccgcat acatgatcca
     3481 cggcatgact gtttgaaact gagtttccgt ataaggctca actacaggaa tacgttgagc
     3541 agattcctga gcataggttt taaatctatt gatgaattgt tcccgcgtcc atgtaccgat
     3601 tccagttttc tcatcgggcg tgatattggc agaacgcaaa acaccttgcg gcattataaa
     3661 ttggaatccg cccgcgaaat ccattccggg caacggttca ccttttttgg tcatcgtatg
     3721 acaatccgcg caggctgcca tattggccaa atatttaccg tatgcgactt catctgtcgt
     3781 gtccggacgc gtcacgaatg caggtgcttt gggcatggtg cgaacgatca ggttgagagg
     3841 gaagttgagc cgcgtttccg gcacttgatt ttcaatcggc ttcaatgttt tgatgtacgc
     3901 aatgatcgac agaatatctt ctttatcgct cgtcgcataa cgatgccacg gcattaacgg
     3961 aaaaatcggt tctctgtttt tccgaatacc tgtcgtgagt gcatgaaaga tttctccgtc
     4021 cgtccaatct tttaaaccgg ccggcgtaat attacgtgcg tacaatgtac cgggaaaacc
     4081 catagattcg ggaaattctt cgccgccggc gccaaatgtt tccggtttga gcgggcctgc
     4141 gaaaaatgaa taatcacgct tcgaatgaca atccgtacat accgctacgt gattggctaa
     4201 atatttcccg cgttcaattt gctccgatga cgatgcgatc accatctgcg gcacgggacc
     4261 tgcattcggc agcgcagttt tgatatatac atatccgcca atgattgcca aaataaatag
     4321 cccaaacaaa ccggcgacaa tacgcaatac aagtttcatg gctttctccg aaaatgggtt
     4381 tacaatttag gtgacttgtt ttttttcgta agcgatgttc tgtctttcac cggaatgact
     4441 aatttcagac cggaccacac atcgtccacc gcgcacactt tgacatcgac caatccgttg
     4501 cggcgtgcac attcacgaat catatcctcc gtaatatcgg tacgaacacc agatgatttt
     4561 ttaggccaag aaacccatat catccccgaa tctttgatct ttttcttaag ctttggaagt
     4621 tcaatctcca aactatcggc cgacttggta aaccaatgga cgcaatcatg atccgctacg
     4681 accttacgtg tcaggcgagc tttttccgga agcggttcga ttaacgcctt caatgtcgca
     4741 ggagcatcaa cccaggcaac tgaatagcct tctttgatac ctattttttt tactaaaggc
     4801 gtacctgaat atccggccaa agaaaatttc tccgtgttaa gagataattt ttaaaataaa
     4861 aaacggtctt gtctgacgtg ttacacgaag ccaaagttaa ttcgaaaaaa atacaccaaa
     4921 attatctttt ttgagaactg tgcagcctaa atttttatac ccggattcaa cgaacaacct
     4981 gatccggcat cgttttcaca ataccgatca ttccgtcggc gacattgtat accgattgaa
     5041 atcctttttg attcataagc gaagccgctt tagcgctgcg gtttccgctc cggcaataca
     5101 cataatacgt cgcagattta tccaattttt ccaattcgga ttcaaattcc ggcttcataa
     5161 aatccaataa cacggcatcc ttcaagtgac cggttgaaaa ttcaccgacc gtgcgcacat
     5221 ccagcaaaac gcccggtttg tcttttagca attggttgag cgtatccgaa ttgacagaac
     5281 gaaaactatc ctgcgctccg cacgctgttg ctagcatgaa aatgataata ccgacgcgat
     5341 acatcattat gaaaaactcc tgatttgatc gttgagtttg tctttgacga cagagcacat
     5401 catatcacac aacgtaaata cggaggtatc ggccacgctg taaaatgtga acaatccttc
     5461 tttcctgcgt ttgagaaagc cgcattcatg caaaacggcg agatgtttgg aaacattgac
     5521 ttggctggtc tgcacttttg ccacaatatc ctgaacgctc atttcgctgt gccaaatcgt
     5581 ttgtatgatt ttaagccgca tgggctctga tagcgctttg aaacgcttgg ctatcaaagc
     5641 cagcgattct tcgctcattt tttctttatc aaataatttt tttgccatag gttggttaac
     5701 ttcgtaaaat ggttactcaa aataaagtca aaatgccaaa atacttgact tcctattaaa
     5761 atatctctat actactatgt agttatataa cgatattcca actaaaagtc aatcggtttg
     5821 gagtgaagca tgtacttcca gaatatttat gaaaaagggc tcgcacaggc cagttatctc
     5881 atcggctgtc aggcaacagg gacagcgatc gtcattgatc cgcgccggga tatccatgtt
     5941 tatctggaag ctgctgcgcg tgaaaaattt acaatcacgc acgtcaccga aacgcacatc
     6001 catgctgatt ttctgagcgg cgcgcgcgaa ttggcggctg cgacaggcgc tgagttgtta
     6061 ttgtcggacg aaggcggtgc agattggcaa tatgcttttc cgcataccgg tcttcgcgac
     6121 ggttcggttt ttaaagtagg caatctgaaa tttgaagtca tgcatacgcc gggacatacg
     6181 ccggagcata tcgccttcgt actcacggac gtgccggccg gcgactaccc gattatgttt
     6241 ttttcaggcg attttgtttt tgtaggcgac atcggtcgtc cggacttact agaaaaagcc
     6301 gcgggtgtta cgggtacgat ggaacgcggt gcgcaacaaa tgtttcattc gttaaaaaaa
     6361 ttcaaatccc ttcccgacca cgttcaggtg tggcccggtc acggcgccgg ttcggcctgt
     6421 ggtaaagcac tgggcgcagt tccgggtact acggtcggtt acgaaaaact ggtcaattgg
     6481 gcgttgcgtt ataccgacga aaatgaattt gtcaaagaac tccttagcgg acaaccggaa
     6541 ccgccatatt attttgcgat gatgaaaaaa ctgaataagg aaggccctcc gattttaggc
     6601 acttcgtccg ggttaaaacg cctgacaact gacgacgcaa cgcaggctat acgcaacggt
     6661 caaactctga tcgacacgcg ccccaaaacc gcatttgctg aaaaacatat acccggcgct
     6721 atcaacattc aaaacaacaa tgccttgtca acatgggccg gatggatatt gtcttacgat
     6781 aaaccgtttc tgattcttgt gtcggaaaat gatgcgcagg atgtatatca taaattaatt
     6841 cgcattggtc ttgatcactg caccggctat atcaccgaaa atcgtttctg ggaagaaagc
     6901 ggcggcacgc tccacagtct ccggcaaatt tttgctacgg atttatctgc gaaattatcg
     6961 gacccgaatg ttgccgtcat tgatgttcgt ggcgtatcgg aatacgagac cggtcatatc
     7021 gcaggggccg tacatattca tggcggtcat ttgtcgcgac ggcttgccga tcttccaaaa
     7081 aacaaaacgc tgattattca atgccagggc ggggaccgct ccagtaccgc taccagcctt
     7141 ttgctcaaaa atggatttag taatatttat aacctgatcg gcggaatagc cgcatggcaa
     7201 aaagccggtt tgccggttgt caaagaaaat taaaggagca gaaaatgccg acgtggatgc
     7261 ttttgttagt tacaggcgta attttatggt ctttatataa aaaaaaatta ggaaaaaata
     7321 ctatgtttca aaatctgaat aatgacgcat ttagtgaaaa aatgaaaaat gctgatcatg
     7381 tggtcttgga tgtacgcacc gatgccgaat acgcctcagg ccacttgccc aaagccaaac
     7441 ttatcaacgt aatggatgca agtttcagca gcaaagtgga tgctttggat aaatctaaaa
     7501 catacctcgt atattgccgt agtggcagtc gttcacgcgg cgcttgtgaa atcatggcca
     7561 gccgcgggtt tcaggttttc aatttagaca atggcatcat gggttggaac gggccggtcg
     7621 aacgttaata tggaatatat aaaaaaacat cgtttttgga tcgggccgtt ggcaggaatt
     7681 atcgcgggat tcttttattg gaaatacatc ggttgtcttt ccggtgcgtg cccgctgcaa
     7741 tccagcttgt gggtcaatct attacttggc gctttgatcg gttatacgat cgccgacacc
     7801 ctacgtacac tgcccgaaaa atcctagttt gacccatccg ttatatttaa aatatatttt
     7861 cttttttaaa gagcagttca aaaaaactgt tctttttttt ggttaatccg aaaataaata
     7921 acagacatta acatgacctt actcatgttt tagataaaac ttgaggtttg ttatctattt
     7981 acatttatga gatttgaatg cttttttgaa acatgaaagt caccgtatgc atagcaacct
     8041 cctgtatggc ataacaccaa tctattgact acagtcattt tgagataacc atacctcccc
     8101 tatctttcca gccgctacgt ttatcttaac atttgttctg ttttcttttt catttctgaa
     8161 cagccgattc ggcttatttt tttacaccgg tattcgaacg aacgttcgaa aaatctctta
     8221 cacgaggcat tggattatgt caacacaaat agacgcatgg caaatgacga cttcgggaca
     8281 accgatgaac cgcgtcgcat cggccatacc ttctttaggt ccggacgaag cactgattcg
     8341 gattgccgca tgcggcgtat gtcatacgga cttgggcttt tattacgacg gcgtccgcac
     8401 agtcagtccc ttgcctttga cgctcggcca tgaaatcagc gggcaggtcg tggataccgg
     8461 tttatccgcg acagcatgga aaaacaaatt cgttcttatt ccggccgtac ttccgtgcgg
     8521 aacttgcgaa atgtgccaag ccggtcaacc acgcatctgc cgtcatcaaa aaatgccggg
     8581 taaccacatc cacggcggtt ttgcttcgca catcgtc
//
LOCUS       DAOQNQ010000125         8512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3070_length_8512_cov_5.596547, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000125 DAOQNQ010000000
VERSION     DAOQNQ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8512
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3070_length_8512_cov_5.596547"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..392
                     /locus_tag="PK754_08865"
                     /pseudo
     CDS             48..392
                     /locus_tag="PK754_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006195273.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
     gene            456..863
                     /locus_tag="PK754_08870"
     CDS             456..863
                     /locus_tag="PK754_08870"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNB09656.1"
                     /translation="MLNSSKAPEPVGLYPHARRVGNLLFLSGVGPRERGKKTIPGVTL
                     DAKGNIKKYDIAKQCHSVFRNIRYILEDAGSSWDNIVDVTVFLTNMKEDFPIYNKIYA
                     EYFKNNKPCRTTLEINALPTPIAIELKVIATID"
     gene            936..2471
                     /locus_tag="PK754_08875"
     CDS             936..2471
                     /locus_tag="PK754_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNB09657.1"
                     /translation="MKKFISPLRILWFCVLAPMLPAQTKLDKDSLSRYANAALAQWNV
                     PGMAISVVLNDSMIYAKGFGVKTFGKNDPVDANTNFSIASLTKAMTAASMGILVDEGR
                     VRWDDPMSNYLPEFKMYDPYVTQAMTIRDLLSHRSGLETFSGDLVWYGTHYDRSEVLR
                     RARYLQPKYGFRAKYGYQNIMYLAAGEVVGRVSGKGWDDFIRERIFIPLGMTRTITRF
                     ADIRTMTNIATPHAIVDFKPVPVEYRNWDNVAPMGSTVSNVVDMAQWIRLQLGRGTYE
                     GRTIYSTAVAREMWQPHTLMDISENGERAMPSRHFLAYGLGWTMHDYHGKKILTHSGG
                     ADGMVSRLVLIPEDRFGFVILTNSINGLTTPMIYHIIDMHLGKNEKDWDAFYWEFNKR
                     AELKEIADRKKTDSLRVKNTKPTLKIEAYAGIYGGELYGDVEVKTEKDKLTIRFLPTS
                     TFTGTLTHWHYDTFQITLQDKNLPTGFATFTLDAAGKPDELKIDIPNPDFDFTELKLK
                     RKR"
     gene            2853..3383
                     /locus_tag="PK754_08880"
     CDS             2853..3383
                     /locus_tag="PK754_08880"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB09658.1"
                     /translation="MESPILTETPRLLIRPMHASDAEMFFDLFGSAETLIHFPRAYTR
                     DEVHRLIRRQQERYTSRGYGLWTLVARDSGEIIGDCGLIPQRVDACEEVELAYHLRKK
                     FWHQGYATEAGAAAIAWVEAHNHAASLIALIRPENVPSRHVAERLGFIKDTAIFHAGL
                     LHDVFRRAIVPQPVSV"
     gene            3436..4827
                     /gene="cysS"
                     /locus_tag="PK754_08885"
     CDS             3436..4827
                     /gene="cysS"
                     /locus_tag="PK754_08885"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011354198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNB09659.1"
                     /translation="MPSLQLFDTYTRALRNFEPLQPGEVGMYTCGPTVYDYAHIGNLR
                     TYIFEDILRRVLEYNGYKVHHVMNITDVGHLTSDADTGEDRMEKGSRRTGKTAWEIAA
                     EYTEEFKKDMKRLNITEPHTWCKATDHIPEQIEFIRGIENKGYTYRTSDGIYFDTSKL
                     DDYGYIARLKVEGLEAGSRVEIGEKRNITDFALWKFSKPEEQRQMEWDSPWGKGFPGW
                     HIECSAMSAKYLGSYFDIHCGGEDHIMVHHPNEIAQTQACYGTRLANFWMHGYFLQID
                     NSRMGKSVGNFLRVQTLIDQGYDPIVWRMFCLSAHYRTKLNFNWESLDGSATALQRLR
                     TAVYEWGAAGTPDEATLTQFRTFINDDLNIPRVLALVWDLVKSDKTSATKKATVLEMD
                     RVLGLGLAAWVPAVEEIPAEIQAWADARVLARKEKRWADADTFRDKITAAGYDIKDTP
                     QGIKVEKRKTPGA"
     gene            4827..5870
                     /gene="dusB"
                     /locus_tag="PK754_08890"
     CDS             4827..5870
                     /gene="dusB"
                     /locus_tag="PK754_08890"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNB09660.1"
                     /translation="MNIDNVNIHDFPVSLAPMEDVTDIAFRIVCKRLGADILYTEFTS
                     SEALIRDARKALRKIQVADEERPVAIQLFGGLDTSMEGAARIAEKANPDFIDINCGCW
                     VKDVAMRGAGAGLLKDLPRFEAIVRSTVRGTQLPVTVKTRLGWDKNTINILDVARMVE
                     DAGAKALTVHCRTRDMGHDGSADWHWLEKIKKVISIPLFGNGDVKTPQDAKRMLETGC
                     DGVMIGRAAIVNPWIFRESRHYIDTGEILPPPDAEERLRVCIEHLRLSVKYKEEKYGV
                     LEFRKYYSGYLKGLPYIGKLRAELMQYTTLQPILDRLAQYGEEIKQWQASEGRDAMLP
                     QLGGLIGTRHETV"
     gene            6109..6441
                     /locus_tag="PK754_08895"
     CDS             6109..6441
                     /locus_tag="PK754_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003009423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNB09661.1"
                     /translation="MVITFKNKKISALVNNDRKLLKEMGKKRADKIRQRLDQLQAAET
                     LEDLRHVAGNYHELTGDRKGQWACDLDQPYRLIFIPHENPVPVNTSGQYIWLEIRGVE
                     IIEITDYH"
     gene            6453..7568
                     /locus_tag="PK754_08900"
     CDS             6453..7568
                     /locus_tag="PK754_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HNB09662.1"
                     /translation="MIRANEYFPQSVTHAGADLDEKLKELGMGVKEFAIRTDKPEKTI
                     IAVIKGDSAITPDMAVQFEQVLKIPAHFWLNRQRQYDESIARSKQKEMIQEAEDWASS
                     FPLKEMLKLKWIRSFSSKSEAVTALFEFFGISSPAAWESLYLRSELKVQFRISLAHTK
                     EHHALSAWLRRGERQAGELDVPDYSEKNFKQALPEIKTVMAKHPKDFFTKLQSLCKEA
                     GVKVVHTPCLPKTPISGATRWLNDTPLIQMSGRYKRNDSFWFTFFHEAGHILLHGKKD
                     IFLENVQYGDRDDEKEEEANFFSQKWVFSESEKTNVLSYADKTGRLTHADVIEFAKEF
                     NTHPAMIIGRLQHDQKIPYSLGREFIKPVELDKNSDC"
     gene            complement(7677..>8512)
                     /locus_tag="PK754_08905"
     CDS             complement(7677..>8512)
                     /locus_tag="PK754_08905"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNB09663.1"
                     /translation="PPILLVICAMTSVQWGAALAKSLFAEFGAITVVFWRVTLAAIML
                     LIMVRPAFRAIRRHEWQVSIVFGIVLVGMSVTYYLSLERLPLGLCVTIEFIGPLAVAL
                     WHSHRALDFIWVALAALGIALLNPFSGHVDPVGFVLALVAGACWGLYIILGEKLGSAT
                     SGSVGLTIAMCVGSLVLIPVKIADAPQWLNIIPVLPFALVVAFLSSVIPFSFEIEALR
                     RMPPKVFGILMSMEPAYAAVIGFLLLDEYLTLSQCIAIGAVVAASVGAARTGSSVPTD
                     V"
BASE COUNT         2388 a         2091 c         2035 g         1998 t
ORIGIN      
        1 atttcggatc gttgattttc gaacgtttac aaaatacaca tagttttatg aattcgatgg
       61 actttaagtt aagaacaaaa gattttgcaa aacgtgttat cgccgtatgc cgggcattac
      121 cgcccaatcg ggagtcgcag ttgatacaac gccaattgtt ccgatcggga acatccgtcg
      181 gagcgaatta ccgtgaagcc tgcagagcac gatctacggc tgaatttata gcgaaattag
      241 gtatcgtaga agccgaagcc gacgaaacac tgtactggct ggaacttatt gcagaactgg
      301 aattacttcc taaaattaaa ctcaaacagc taattatcga aaatcacgag atcatcgcta
      361 tgacagttgc gtcaattaaa acattaaaac aaaacaaaac gggagataaa aaaaccgaca
      421 ctccccaatc cccaatccca aatcctaaat cgggaatcct gaattcctcc aaagctccgg
      481 aaccggtcgg cctgtatccg catgcgcggc gcgtgggcaa tctattattc ctgtcgggcg
      541 tcggaccgcg agaacgcgga aaaaaaacca tccccggcgt tacactggat gccaaaggta
      601 atatcaaaaa atacgatatc gccaaacaat gtcattccgt gtttcgcaat atacgctata
      661 tccttgaaga tgccggttcg tcctgggata atatcgtgga cgtcacggta tttctgacca
      721 acatgaaaga agatttcccg atttataata aaatttacgc ggaatatttt aaaaacaata
      781 aaccctgccg cacgacgctg gagatcaacg cgctgcctac acccatcgcg atcgaactta
      841 aagtcatcgc aacaattgat tagttgagat ggtaacttta aaggctaccg tgcgtaaaac
      901 gatacattaa aactctatta gtgagagagc actgcatgaa aaaattcatt tcgcccttgc
      961 gcattctttg gttttgcgtg ttagcaccga tgcttccggc ccagaccaaa ctggataagg
     1021 atagtctttc ccgctatgca aacgccgcgc tcgcgcagtg gaatgtaccc gggatggcca
     1081 tttccgtcgt actcaacgac tcgatgattt acgccaaagg cttcggtgta aaaaccttcg
     1141 gaaaaaatga tcccgtagat gccaatacta atttttcgat agcctcgcta accaaagcca
     1201 tgacggcggc gagcatgggc attctggtgg atgagggtcg cgtgcgttgg gatgacccga
     1261 tgtccaacta tcttccggaa tttaaaatgt acgaccctta cgtcacgcag gccatgacca
     1321 tccgtgattt attgagccat cgtagcggtt tggaaacatt tagcggcgat ctggtgtggt
     1381 atggtactca ttatgaccgt tcggaagtgc tgcgccgggc aagatatttg caacccaaat
     1441 acggcttccg tgccaaatac ggttatcaaa atatcatgta tctcgccgca ggagaagtcg
     1501 taggacgcgt atccggtaaa ggttgggatg attttattcg tgaacgtatt tttatccctt
     1561 tgggtatgac ccggacgatt acacgttttg ccgatatccg cacgatgacc aatattgcta
     1621 caccgcatgc gatcgttgat ttcaaacccg tgcctgtcga atatcgtaat tgggataatg
     1681 tcgcaccgat gggctccacc gtttccaatg tggtagatat ggcacagtgg attcgcctcc
     1741 agttgggacg cggcacttac gaagggcgga caatttatag caccgccgtc gcgcgtgaaa
     1801 tgtggcaacc gcacaccctg atggatattt cagaaaatgg agaacgcgct atgccgtccc
     1861 ggcatttttt agcttatggc ttgggctgga ctatgcacga ctaccacggt aaaaaaatac
     1921 taacacactc cggtggcgcc gacggtatgg tctcacggct ggttcttatc cccgaagacc
     1981 gtttcggttt tgtgatactc accaatagta ttaacggcct caccacaccg atgatctatc
     2041 acatcataga tatgcacttg ggtaaaaacg aaaaagactg ggatgctttt tattgggaat
     2101 tcaataaacg cgccgaactg aaagaaattg ccgatagaaa aaagaccgac agtctgcgcg
     2161 tgaaaaacac caaaccgacg ctcaaaatcg aagcgtatgc cggtatttac ggcggtgaac
     2221 tctacggtga cgtcgaagtc aaaacagaaa aagacaaact gacgattcga tttttgccga
     2281 cgtccacttt caccggtacg ctcacgcatt ggcactatga cacgtttcag atcacgcttc
     2341 aggataaaaa tctaccgacc ggatttgcca cctttacgct ggatgccgcc ggcaaaccgg
     2401 atgaactgaa aattgacatc cccaacccgg attttgattt tacggaattg aaattgaaac
     2461 gaaaaagata agataatgtg tgtttttacg caccaacatc aaatgcatgt agagtttcca
     2521 aaccggcacg gagtattaaa tttggatagg gtcaattagg ccttaagaaa aaggccgctc
     2581 ggaactctcg gttgcgcatt atcatcggcg aaaaacctgt ttgacctcgc catcggcggg
     2641 ggagttgttt ttcgccacca gtaagcaacc gagggtttag gcgtttttct cacagccttg
     2701 atttttcttt ggttactttc tttgcatcaa ggcaaagaaa gtaacagctt ggaattcgac
     2761 gccaaacata tatgccattg aaacaaattt cgttgcttgc ctgatacgtt ttaggttgac
     2821 ataacggtac ttagcgttta ttttgcgcgg cgatggaatc tccgatccta actgaaacgc
     2881 cgcgtctgtt gattcgcccc atgcacgcat cggatgcgga aatgttcttt gatttattcg
     2941 gctccgcgga aacgctgatc cattttccgc gtgcgtacac ccgtgacgaa gtgcatcggt
     3001 tgatccgtcg ccagcaagaa cgctacacat cacgcggtta tggcctctgg actttggtgg
     3061 cgcgcgactc cggagagatt atcggggatt gcgggttgat acctcagcgt gtcgatgcat
     3121 gcgaggaagt agaactggct taccatctcc gcaaaaaatt ttggcatcag ggttatgcca
     3181 cggaagccgg tgccgcggcc atcgcatggg tcgaagccca taaccatgcc gcttcgctca
     3241 tcgcactgat caggcccgaa aatgtaccca gccgccatgt tgcagaacgg cttggcttta
     3301 taaaagacac ggcgattttt cacgccggac tgctgcatga cgtatttcgt cgtgcgatag
     3361 ttccacagcc ggtgagcgta taattttttg acataacatt attgcattat ccattttata
     3421 cccgttacaa cacccatgcc ttcattacaa ctttttgaca cgtacacccg cgcattgcgg
     3481 aattttgaac cgctccaacc cggcgaagtc ggcatgtaca catgcgggcc tacggtttat
     3541 gactacgcgc acatcggcaa tctacgcact tacattttcg aagacattct gcgacgtgta
     3601 cttgaataca acggatacaa agttcatcat gtcatgaata tcaccgacgt cgggcatctc
     3661 acatcggacg cggataccgg cgaagatcgc atggaaaaag gttcccgtcg taccggtaaa
     3721 acagcatggg aaatcgccgc ggaatatacg gaagaattca aaaaagatat gaaacggtta
     3781 aatataaccg agccacatac ttggtgcaaa gcgaccgatc atataccgga acagatcgaa
     3841 tttattcgcg gcatcgaaaa caaaggatac acctaccgca catcggacgg catttatttt
     3901 gacacatcca aactcgatga ctacggatat atcgcgcggc tcaaagtcga aggcctcgaa
     3961 gcgggttcgc gcgtagagat cggcgaaaaa cgcaatatca ccgactttgc gttgtggaaa
     4021 ttcagcaaac ccgaagagca gcgtcagatg gagtgggaca gcccgtgggg caaaggtttt
     4081 ccgggatggc atatcgaatg ttccgccatg tcggcgaagt atctcggttc atactttgac
     4141 attcattgcg gcggtgaaga tcatatcatg gtgcatcacc ccaatgagat cgcgcagacg
     4201 caggcttgtt acggaacgcg tttggccaat ttttggatgc acggttattt tcttcagatt
     4261 gataattcgc gtatgggcaa atccgtcggc aattttttac gtgtgcagac actcattgat
     4321 cagggatacg atccgatcgt gtggcgcatg ttttgtctca gcgcgcatta ccgtaccaaa
     4381 ctcaatttta actgggaaag cctcgacggt tcggcaacgg cgctacaacg tctgcgcacg
     4441 gcagtgtacg aatggggcgc ggcaggcaca ccggatgaag cgacgctcac gcagtttcgc
     4501 acgtttatca atgatgatct caatattccc cgtgtactcg cgctggtgtg ggaccttgta
     4561 aaaagcgaca aaacttcggc tacaaaaaaa gcgaccgtac tggaaatgga tcgcgtgctg
     4621 gggctcggtt tggcggcgtg ggttcctgcg gtcgaagaga ttccggccga aatccaagct
     4681 tgggccgatg cccgcgtact ggcacgcaaa gaaaaacgct gggccgatgc cgacacgttt
     4741 cgcgataaga tcacagccgc gggttacgat atcaaagata ccccgcaagg catcaaagtc
     4801 gaaaaacgta aaacaccggg cgcctgatga atatagataa cgtcaacata catgattttc
     4861 ccgtctcgct ggctccgatg gaagatgtca ccgacattgc ctttcggatc gtatgcaaaa
     4921 ggcttggcgc cgatattctt tatacggagt tcacttccag cgaagccttg atccgcgatg
     4981 cccgcaaagc tttacgcaaa atccaagtcg ccgacgagga acgccccgta gccatacagc
     5041 ttttcggcgg attggatacg tccatggaag gcgcggcgcg gattgctgaa aaagccaatc
     5101 cggattttat agatatcaac tgcggctgct gggtcaaaga tgtcgccatg cgcggcgccg
     5161 gggccggtct gctcaaagat ctgccgcgct tcgaagcgat cgtacgctcc accgtgcggg
     5221 gcacgcaact tccggtcacc gtaaaaaccc ggctgggctg ggacaaaaat acgatcaaca
     5281 ttctcgatgt ggcgcgtatg gtcgaagacg cgggcgcgaa agcgttgacc gtgcactgcc
     5341 gcacacgcga tatgggacac gacggttccg ccgactggca ttggttggaa aaaataaaaa
     5401 aagtgatttc gataccttta ttcggcaatg gcgatgtcaa aacgccgcag gatgccaaac
     5461 gtatgctgga gaccggttgt gacggcgtga tgatcggccg agcggctatc gtcaatccgt
     5521 ggatttttcg cgaatcgcgg cactacatag ataccggcga aatcctgcct ccgcccgatg
     5581 ccgaagagcg gttacgggtt tgtatcgaac atctgcggtt atctgtcaaa tacaaagaag
     5641 aaaaatacgg tgtgctggaa tttcgtaaat attactccgg ttatctcaaa ggccttccct
     5701 atatcggcaa actgcgcgcc gaactgatgc agtacacgac actgcaaccc attctcgatc
     5761 gcctcgccca atacggcgaa gagatcaaac aatggcaagc ttccgaagga cgcgacgcca
     5821 tgcttccgca actcggcggc ctcatcggca cgcggcatga gacggtatga gtaagtatat
     5881 tttttattgg taaattttct atcggattca cgcgatgagt accaaatttt ttacaaacga
     5941 agaagagaac acgcttctca ccaagctcga aagcatcttt caccatcgaa atatctacta
     6001 ttttgatgca ctggtgggat ttttgtgaag tgaaataggc ttaccgtcac ctttggtgtt
     6061 acttttttaa tttacactta gtgtttatta taattgactt tttaagtcat ggttattaca
     6121 tttaaaaaca aaaaaatatc agcactcgtt aataacgaca gaaagctttt gaaggagatg
     6181 ggtaaaaagc gagctgacaa aattcgtcaa cgccttgatc aacttcaagc cgccgaaaca
     6241 ttggaggact tacgccatgt ggccggcaac tatcacgaac tcacgggtga ccgtaaaggg
     6301 caatgggctt gcgatcttga tcagccctat cggctgatct ttataccgca cgaaaaccca
     6361 gtaccggtta atacatcagg tcagtatata tggctggaga ttcggggtgt tgagattatt
     6421 gaaatcactg actatcattg aaaagaggac ttatgattag agccaatgag tattttcccc
     6481 aatcggtcac ccatgccggg gctgatcttg atgaaaaact caaagaactc ggtatgggag
     6541 tgaaggaatt tgctatccgt acagacaaac ccgaaaaaac gatcattgcc gtgatcaaag
     6601 gcgatagcgc catcacaccc gatatggctg ttcaatttga acaggtctta aaaatcccgg
     6661 cgcatttttg gcttaatcgc caacggcaat acgacgaatc tatcgctaga tcgaaacaga
     6721 aagaaatgat ccaagaagcg gaagactggg cgtcaagctt tcccttgaaa gaaatgttaa
     6781 aattaaaatg gatacgttct ttctcaagca aatcggaagc ggtaactgcc ctctttgaat
     6841 tttttggaat ttcgagcccg gcggcatggg agagtctgta tctaaggtca gaactgaaag
     6901 tccaattccg catttcgctt gcccatacca aagaacatca tgccctttcc gcctggttga
     6961 gacgcgggga aagacaagcg ggagaactgg atgtgccgga ttattctgaa aaaaacttta
     7021 aacaggcatt gccggaaatc aaaacagtca tggccaaaca tccgaaggat ttttttacaa
     7081 aattgcaatc gctctgcaaa gaagccgggg tcaaagtcgt ccatacccca tgtcttccca
     7141 aaacgccgat tagcggcgcg acacgctggc ttaatgacac cccgctgatt cagatgtccg
     7201 ggcggtacaa gcgcaatgac agtttttggt tcactttctt tcacgaggca ggacatattc
     7261 ttcttcacgg gaaaaaagat atttttctcg aaaatgtaca atacggagat cgcgatgatg
     7321 aaaaagaaga agaagccaat ttcttttcac agaaatgggt ttttagcgaa tcagaaaaaa
     7381 ccaatgtttt atcctatgct gataaaaccg gtagattaac acatgcagat gttattgaat
     7441 ttgctaagga attcaacacc catcctgcaa tgattatagg ccggcttcag catgaccaaa
     7501 aaatccccta tagcttgggt cgggaattta tcaagccggt agaattagac aaaaactcgg
     7561 attgctgatc tcaacgccaa tcctcatttt tttgacgctc actctcgttg catccttttt
     7621 tttcataacg tgcaaatcat cggatcgcgc tgaatgcgat agtttcgttt atccccttac
     7681 acatccgtcg gcacgctgga cccggtacgt gccgcgccga cacttgccgc tacgaccgcg
     7741 cctatcgcaa tgcattggga cagcgtaagg tattcgtcga gcagcaaaaa accgatcacc
     7801 gcagcgtacg caggttccat actcatcagt atgccaaaaa ctttgggagg catacggcgc
     7861 agtgcttcga tctcaaaact gaacgggatc accgaggaca aaaacgcaac cactaaagca
     7921 aaaggtagta cgggaatgat attgagccac tggggcgcgt cggcgatttt gacgggtatc
     7981 agcaccaaac tgcctacaca catcgcgatc gtgagaccga cggagcccga agtcgcactg
     8041 cccaacttct caccgagtat gatgtacagc ccccagcatg cgcccgccac aagcgccaat
     8101 acaaaaccga ccgggtccac atgcccgcta aacggattga gcaacgcgat gccgagcgcg
     8161 gctagcgcca cccagataaa atctagagca cggtgactgt gccaaagcgc cacggctaaa
     8221 ggaccgataa actcgatagt gacgcacaaa cctagcggca aacgctccag cgataaataa
     8281 taggtgacgc tcattccgac cagcacgatg ccaaaaacaa tactaacctg ccactcgtga
     8341 cgacggatcg ctcgaaaagc cggacggacc ataatgagca gcatgatggc cgctaaggtg
     8401 acgcgccaaa aaactaccgt gatcgcgcca aattccgcaa aaagggattt tgcaagcgcg
     8461 gcgccccact gtacgctggt cattgcacaa ataaccaaaa gaatcggagg aa
//
LOCUS       DAOQNQ010000126         8509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3072_length_8509_cov_5.828838, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000126 DAOQNQ010000000
VERSION     DAOQNQ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8509
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3072_length_8509_cov_5.828838"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1448
                     /locus_tag="PK754_08910"
     CDS             <1..1448
                     /locus_tag="PK754_08910"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNB09664.1"
                     /translation="AFPFAQRISLDGKPVLPAFTDSHTHFLSHCLQQAQIDLSGLTSV
                     GACLNVIGQKAATTPEGTWIKGKGWNQNLWSPAHYPTKEQLDSVTRQHPIALDARDFH
                     SLWVNSLALAQAGINEQTVFNGAGEICKNENGQLTGIIKEDARTLVWKIMPEDTAEQR
                     AEVLRNLQTLAYKNGLAGVHCMETMPDFEAYQSLHRDRALQLRVCFYLPIRYLDMMVE
                     AKIKSGFGDAYFRFGGMKIFMDGTLGSQTAHMLEPFENSENYGTEITTQDALDAYVLR
                     AAEYDIACAIHAIGDRANRKVLNAFEKIREMFPDKKLRQRIEHAQIIHPDDIQRFARS
                     GTIASMQAIHIPEDIDAANRYWGARARWAYPFRSLLQSGATIALGSDVPIETCNVFEG
                     MTAALCRTRRTSDEVWYPEECMTREEIIYAYTVGAALASGEEHLKGTLSSGKLADFMV
                     LSQDIFSVQPEKIAGTKVQTMVIGGNVVYES"
     gene            1458..2222
                     /gene="lpxA"
                     /locus_tag="PK754_08915"
     CDS             1458..2222
                     /gene="lpxA"
                     /locus_tag="PK754_08915"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNB09665.1"
                     /translation="MIHLTALVSESLDTSIQVGAYSIIDDGSQIGKHTIIHNHVRVTS
                     FTVIGQGVVIHQGAALGGDPQDLKFGGEYSTVEIGDETVIREYVTINRGTGTNGKTKI
                     GSHSLIMAYAHVAHDCTIGDHVIISNAVNMAGHVHIEDYAGIGGMVPIHQFCRIGRYA
                     FVGGGFRVNQDVPPYVLAAGEPLRFSGLNYVGLRRNGFSEERIKVIDKAYFILYRQGL
                     TRRDALKKMDDEFGTNPDVLNIIQFVETCERGLIKP"
     gene            2232..2657
                     /locus_tag="PK754_08920"
     CDS             2232..2657
                     /locus_tag="PK754_08920"
                     /inference="COORDINATES: protein motif:HMM:NF024559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3995 domain-containing protein"
                     /protein_id="HNB09666.1"
                     /translation="MRLIWIIFNTSVFLFLSGLHFYWLKENPRRVFAVIPETNEGERL
                     FKPGIVATSIVAMGLLIFALITAGQTGIYQDWLDTKLIQYGTLGIAVVFALRTVGDFK
                     YVGIFKTVRKTLFAINDTRYFVPLCAILSGGSFYLYFSN"
     gene            complement(2671..2880)
                     /locus_tag="PK754_08925"
     CDS             complement(2671..2880)
                     /locus_tag="PK754_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013663594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNB09667.1"
                     /translation="MMTLKFKTNIKCGGCVATVTPHLDSEKTIEKWHVDLQSPERFLT
                     VEGSEPNEEMIFEKVKQAGFTIEKA"
     gene            complement(3252..5633)
                     /locus_tag="PK754_08930"
     CDS             complement(3252..5633)
                     /locus_tag="PK754_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNB09668.1"
                     /translation="MHSSHCAGIIENELRKLNGVQKADVDFANRRLIIRFYNDIVSIH
                     TIIRKITDLGYGVNYTKKTLGVQGMTCAACAASVESMLLSQEGIFNAQVNFAAKTVFV
                     EYASDRINVAEMKKALQQIGYDISGDMESDADQIEVAEKKRYNELRLRMLVAILLSLP
                     VFTIGMFFHNTWTWGNWISLILSAPVLFWAGRDFYINAFKQARHGTANMDTLVALSTG
                     VAFIFSLFNTLFPHFFHQQGLHADVYYEAAVVIIALILLGRWLEEGAKNKTNSAIKKL
                     MGLQPKTVRVLRDGNTLEIPVRDVQIGDLVIIRPGEKIAVDGTVTDGNSFVDESMISG
                     EPIAVEKKSSSKVFAGTINQNGSLTIQAEKVGRATLLSQIIRAVQEAQGSKAPIQKTA
                     DRIAAVFVPVVIGIALMSGMLWYVLGPEPALTRAMLAVVTVLIIACPCALGLATPTAI
                     MVGIGKAASHGILIRDAQSLEIAHRVDTVVLDKTGTITEGKPKVTDIVWSPETNKDTF
                     TSILFAIESRSDHPLAGAVVRHLKIESNDNPIITDLQNTPGRGVSASVNDHRYYVGSE
                     AWVNELRVPIIDTIQLSAQNMINKAQTVVYFFDDKSVLAVIAIADTIKPSSAAAVTEL
                     HRLGIEVHMLTGDHGTSAEYIAKQAGISTVRSGVMPHEKGQYIKSLQAQGKVVAMVGD
                     GINDAEALALADVSLAMARGSDIAMDTAKITLMNSDLKHIASAMQTSKSTVITIKQNL
                     FWAFIYNIIGIPIAAGVMFPFFGFTLNPMIAGAAMAFSSISVVTNSLRLAAKR"
     gene            complement(5894..6445)
                     /locus_tag="PK754_08935"
     CDS             complement(5894..6445)
                     /locus_tag="PK754_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB09669.1"
                     /translation="MTPFLQPTLQNERIYARPLQYEDFDALYAVASDPFIWEQHPNKD
                     RYKRDVFENYFRGAMESGGAFLICDAATRQVIGSSRYCNWNAETRTIEIGYTFIARDH
                     WGTTYNRALKTLMLDHAYTFADAVQFFIGAVNVRSQNAITKLGAQKIAERETAYYGES
                     KKLDFIYEISKSTWTAIRDSKQL"
     gene            complement(6468..6977)
                     /locus_tag="PK754_08940"
     CDS             complement(6468..6977)
                     /locus_tag="PK754_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase"
                     /protein_id="HNB09670.1"
                     /translation="MIVRLAEGTIKTLYGAYREYLYYDGQKESIALVMGDVRGENILC
                     RVHSSCLYGHAFNSMECDCREQMQISQKLIEKAGKGIVIWLEQEGKGNGHYALMQSKK
                     YKQQGMPQGEAYEAAGFSRDARDFRCAAEIIRDLGVSSICLITDNADKVQTLTQHGII
                     VSSTHPVAF"
     gene            complement(7006..7407)
                     /locus_tag="PK754_08945"
     CDS             complement(7006..7407)
                     /locus_tag="PK754_08945"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNB09671.1"
                     /translation="MIAYIFQFVSLTLLGIAYYLVRRKQNFIAECDQVEGTVVRFRES
                     RRPGKNTTYAPVVEYFAYGKSYQHESSLYRSSPGFEIHQTVSVLYKRDKPEEAIIDSF
                     LEKWFIPMILGIVGSVFLIIGAVFPLLEREI"
     gene            complement(7420..7836)
                     /locus_tag="PK754_08950"
     CDS             complement(7420..7836)
                     /locus_tag="PK754_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB09672.1"
                     /translation="MKSYTTSLEIILYVKDQTKSRDFYRNILQREAVLDVPGMTEFLL
                     TDTCKLGLMPTHGIAKIIGDRMPHPDTGVGIPRCELYLFVENAHEQLENALRHGAAFI
                     DPIAERDWGDTVGYVADPDGHVIAFASKSKPETYDY"
     gene            complement(7875..8213)
                     /locus_tag="PK754_08955"
     CDS             complement(7875..8213)
                     /locus_tag="PK754_08955"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB09673.1"
                     /translation="MRNKKKRLRVRDVQDVLDVIGGRWRGPILASLCEKQKRFNELQR
                     DLHPITPRILTRELKYLEMNKLVFRSANGKTSSVVVYGLTPHGQSLDPVIDGIVAWAQ
                     KHRKEVLGYK"
     gene            8309..>8509
                     /locus_tag="PK754_08960"
     CDS             8309..>8509
                     /locus_tag="PK754_08960"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNB09674.1"
                     /translation="MKISVIGASSGVGLQCVQRALQRGHAVTTLSRSPLPVNPSNDLL
                     IIRGSASNKEDIKKTIKDADAVL"
BASE COUNT         2379 a         1826 c         1862 g         2442 t
ORIGIN      
        1 cggcttttcc ttttgcacag cgcatttcat tggacggaaa gccggtttta cctgctttta
       61 ccgatagcca cactcatttt ttgtcacact gcttacaaca agcacaaatt gatttgagtg
      121 gtttgacatc ggtcggagcc tgcctgaatg tcatagggca aaaagcagcg accactcctg
      181 agggaacttg gatcaagggc aagggttgga atcaaaatct atggtcacct gcacactatc
      241 cgacaaaaga acaacttgat agtgttaccc gtcagcatcc catcgcacta gatgcccgcg
      301 actttcattc gctgtgggtc aattcgcttg cactagccca agccggtatc aacgaacaga
      361 cagtatttaa tggtgccgga gaaatatgta aaaatgaaaa tgggcagctt acaggtatta
      421 tcaaagaaga tgcgcgcaca ttggtatgga agatcatgcc ggaagatacg gccgagcaac
      481 gtgcagaggt attacgcaat ttgcagacat tggcatataa aaacggtttg gccggtgtgc
      541 attgcatgga aaccatgcct gattttgaag cctaccaatc tttgcaccgg gacagagcgc
      601 ttcagttacg cgtttgtttt tatcttccga tacgatatct cgatatgatg gtcgaagcca
      661 aaatcaaaag cgggttcggt gatgcctatt tccggtttgg cggaatgaaa attttcatgg
      721 atggcacact gggatcacag accgcacaca tgttggaacc ttttgagaat tcggaaaatt
      781 atggaactga gatcacgacg caggatgcac ttgacgcata cgttttacga gcagctgaat
      841 atgacatagc atgcgcgatt catgccatcg gagacagagc gaatcgcaag gtgttgaatg
      901 catttgaaaa aatacgcgag atgttcccgg ataaaaaatt aaggcaacga atcgaacatg
      961 cacaaatcat tcatcctgat gatatacaac gtttcgcccg gagtggaacc atagcttcga
     1021 tgcaggctat acacatacct gaagatattg atgcggccaa ccggtattgg ggcgcccgtg
     1081 ctcgatgggc atatcctttt cgtagtttgc tccaaagcgg cgcaacgatt gccttaggct
     1141 ctgatgtacc gatcgaaacg tgcaatgtat tcgaaggtat gacagccgct ttatgccgaa
     1201 cccgtaggac ttccgatgag gtgtggtacc cggaagaatg catgacgcgt gaagaaatta
     1261 tttatgccta taccgtagga gccgcattgg ccagcgggga agaacatctc aaaggtacgt
     1321 tgtcgtccgg taaactggct gattttatgg tgctgtcgca ggatattttt tccgtacagc
     1381 ctgagaaaat tgccggcacc aaagtacaaa ctatggtgat cggtggtaac gtcgtatatg
     1441 aaagttgatt ttttgatatg atacatctta cagctctggt ttccgaatcg ttagacactt
     1501 ccatacaagt cggcgcgtat tcgatcattg atgatggctc acaaatcgga aagcatacca
     1561 taattcataa tcatgtacgt gttacatcat ttacggtgat tggtcagggc gtagtaattc
     1621 atcagggtgc ggcacttggt ggtgatcccc aagaccttaa attcggtggg gagtattcaa
     1681 cggtagaaat cggtgatgaa accgtgattc gcgagtatgt aacgattaat cgcggaaccg
     1741 gcacaaatgg aaaaacaaaa atcggttcac attctttgat tatggcgtat gcgcatgttg
     1801 cgcatgattg cacgatcggc gatcatgtta ttatttccaa tgccgtaaac atggcggggc
     1861 acgtacatat cgaagattat gcgggtatcg gagggatggt acccatacat caattttgtc
     1921 gtatcggtcg ttatgcgttt gtcggcggag gatttcgggt taatcaggac gttccgccct
     1981 atgtgttggc tgccggggaa ccgttacgtt tttccggttt aaactatgtc ggcttacgtc
     2041 gcaacggttt ttctgaagaa cggattaaag ttatagataa ggcttacttt atcttatatc
     2101 gccaaggcct gacgcgtcgc gatgcgttaa aaaaaatgga tgatgaattt ggcactaatc
     2161 cggatgtact aaacattata caatttgtag agacatgcga gcgaggattg ataaaaccgt
     2221 agggatgtga tatgaggttg atttggataa tcttcaatac ttcagttttt ttgtttctat
     2281 cgggattgca cttttattgg ctcaaagaaa atccgcgtag ggtatttgcc gttataccgg
     2341 agacgaacga gggtgaacgt ctatttaagc ctggcattgt ggcaacaagt attgtcgcga
     2401 tgggattgtt gatatttgct ttgataacag ccggtcaaac aggaatttac caagattggt
     2461 tggacaccaa gttgatccaa tatggaacac tgggaattgc tgtcgtattt gctttgcgaa
     2521 ctgtcggaga tttcaaatac gtaggaatat tcaaaactgt tcgtaaaaca ctgtttgcca
     2581 tcaatgatac acgatatttt gtgccgcttt gtgcaatatt gtctggcgga agcttttatc
     2641 tctatttttc aaattagaat caacagttga tcatgctttc tctatcgtaa aaccggcttg
     2701 ttttaccttt tcaaaaatca tttcctcatt cggttcagaa ccctctactg tcaaaaatcg
     2761 ttccggtgat tggagatcca catgccattt ttcaatcgtt ttttcactgt caagatgcgg
     2821 cgttaccgtc gcgacgcatc cgccgcattt gatgttggtt ttaaatttaa gggtcatgat
     2881 gtgctccttt aatagtaaat taatataaaa acccgttgtg tgaattaaac aaaagtgaat
     2941 ttgataagat ttaattttta tggtttagta ggttatcaat tgagaaagaa tcattataga
     3001 gtctcaattt gtatagatac ctttcccgct ctcccttcga ttttgttcag ggcatgcgtg
     3061 ggaatgacgc gggtgagaca gcgcagccgt cgccccgttt tttgtgttat agtgtgtttt
     3121 gtatttgatc aagtaatgcc aatgaatcaa atttcacaca aagtatgatc aaaacaacca
     3181 cacgtgaggt taaaatacat gtaaattcaa gtatgagacc actaaataac tcacacaacg
     3241 ggttataaaa cttatcgctt agccgcgagg cgcaagctat tggtaacaac ggatatcgaa
     3301 ctgaacgcca ttgcagcgcc ggcgatcatc ggattgagtg taaatccaaa aaacggaaac
     3361 atcacaccgg cggctatggg aataccaatg atattataaa taaaagccca aaaaagattt
     3421 tgtttgatcg taatcacggt tgatttagat gtttgcattg ctgaggcaat atgcttcaaa
     3481 tccgagttca tgagtgttat cttggccgta tccatcgcaa tatcgctccc gcgggccatc
     3541 gctaaactca catccgccaa agccaaagct tcggcatcgt tgatgccgtc acctaccatg
     3601 gcgaccactt ttccttgagc ttgtaatgac ttaatatatt gacctttctc atgcggcatt
     3661 acaccggacc gaactgtact tataccggct tgtttggcta tgtattctgc actagtacca
     3721 tgatcaccgg tgagcatgtg cacttcgatc cccaaacggt gaagttcggt tacggccgct
     3781 gcgctcgacg gttttatcgt gtcggcgatg gcgatcacag ctaaaactga tttatcgtca
     3841 aaaaaataaa ccaccgtttg cgctttatta atcatgtttt gtgctgataa ctgaatcgta
     3901 tcaataatag gaacacgcag ttcattgacc cacgcctcgc tgcctacata atatcggtgg
     3961 tcgttgactg aagccgacac accgcgaccg ggtgtatttt gtagatcggt gatgatgggg
     4021 ttatcgttag attctatttt caagtgacgg acaaccgcgc cggccaaagg atgatccgag
     4081 cgcgactcta tagcaaacaa gatagaagtg aaggtatcct tatttgtttc aggtgaccaa
     4141 actatatccg taaccttagg ttttccttcg gtgatcgtgc ctgttttatc taaaacaaca
     4201 gtatcaacac gatgggctat ttctaaactt tgtgcatcgc gtatcaaaat cccgtgagaa
     4261 gcagctttac cgatgccaac cattattgct gtcggtgtag ctaatccgag agcacaaggg
     4321 cacgcaatga tcaataccgt aacgacggca agcatagcgc gtgtcagtgc aggttccggc
     4381 cccaacacat accagagcat accgctcatg agcgcgatcc cgattaccac tggaacaaaa
     4441 acagccgcaa tacgatctgc tgttttttga ataggcgctt tacttccttg cgcttcctgt
     4501 acggccctga ttatttgcga cagcagagtt gctcgaccga ctttttcagc ttggatcgtc
     4561 aaacttccgt tttggttgat cgttcctgca aaaactttcg aagaagattt cttttcgaca
     4621 gcgatgggtt cgccgctgat catgctttca tcaacaaatg aatttccgtc tgtaaccgta
     4681 ccatccacag cgattttttc accgggccgt atgatcacta gatctccgat ctgaacatca
     4741 cgaacgggaa tttctaatgt atttccatcc cgcaggacac gaactgtttt gggctgtaaa
     4801 cccataagtt ttttgatagc gctattggtt ttgtttttag caccttcttc gagccatcgg
     4861 ccaagtaata tcaaggcgat gatgacaacg gcggcttcat aatatacatc ggcgtgcaaa
     4921 ccttgctgat gaaaaaaatg aggaaagagg gtattaaaaa gagaaaaaat aaaagctacg
     4981 ccggtactga gagctaccaa agtatccata ttggcggtac catgacgcgc ttgtttgaac
     5041 gcattgatat aaaaatcacg accggcccaa aacaaaaccg gggctgataa gatcaaactg
     5101 atccagtttc cccaagtcca tgtattgtga aaaaacatcc ctatggtaaa aacaggtaat
     5161 gacaacagaa tggctacaag cattcggaga cgaagttcat tataacgctt tttttcggct
     5221 acttcgattt ggtcggcatc gctttccata tccccgctga tatcgtatcc gatctgttgc
     5281 agcgcttttt tcatttcagc gacatttata cgatcggaag cgtattcgac aaataccgtt
     5341 ttggcagcaa aatttacctg cgcattaaag ataccttctt gggaaagcaa catagattcg
     5401 acactagccg cacatgctgc gcacgtcatg ccctgcacgc ctaaagtttt tttagtgtag
     5461 ttaacaccat atccgagatc tgttattttc cggataatcg tatggatcga aacaatgtcg
     5521 ttatagaaac gaattatgag acggcgattt gcaaaatcga catccgcttt ctgcacacca
     5581 ttgagcttac gcagttcatt ttcgataata ccggcacaat gcgagctatg cattcccgat
     5641 aacgataatt catgcgtgat ggattcagat gttttggatt tcgcaagtgc aggttgtaaa
     5701 acctgaagtt gcagagattt tggagaggag ctattcattt ataaataccg gttgaataaa
     5761 ataatctgcc ataatatacg gcggtgatta aaaaaagcgt tatatctgga tggaaatttg
     5821 ttatatctaa ttggatacaa aaaacaggca aaactcaaac gatgagaatt cgaagagcta
     5881 ttacagtatt aaatcaaagt tgtttggagt cgcgtatggc agtccaagtt gatttcgata
     5941 tttcatatat gaagtccaat tttttggatt cgccataata cgctgtttcc cgctctgcaa
     6001 ttttttgtgc tcctaatttg gtaatcgcat tttgcgaccg cacattgact gcaccgataa
     6061 aaaattgaac cgcatcagca aaagtatatg catgatccag catcagtgtt ttgagcgcgc
     6121 ggttataggt ggtaccccaa tgatcgcgtg caataaacgt ataaccgatt tcgatggttc
     6181 gtgtttctgc attccaattg caatatcgtg aactgccaat aacttgacgt gttgccgcgt
     6241 cacaaatcag aaatgcgccc ccggattcca tagccccgcg aaaataattt tcaaaaacat
     6301 ctcttttata ccggtcttta ttagggtgtt gttcccaaat aaacggatca ctggcgacgg
     6361 cataaagcgc atcaaagtct tcgtactgca acggccgcgc gtagattcgt tcattttgga
     6421 gcgtgggttg taagaaaggc gtcataatta cttttattga tgatttatta aaaagcaacc
     6481 ggatgagtgc tgctaacgat aattccgtgc tgcgttaagg tttggacttt atccgcatta
     6541 tcagtaatca ggcaaatcga agatacgccc agatcgcgta ttatttcagc tgcacaccga
     6601 aagtctcgtg catctcgact gaatccggcg gcttcgtaag cctcgccttg cggcatgcct
     6661 tgttgtttgt attttttgct ttgcatcaaa gcgtagtgcc cgtttccttt tccttcttgc
     6721 tcaagccaga ttactatccc tttgccggct ttttcgatga gtttttgtga gatttgcatt
     6781 tgttcacggc aatcacattc catactgttg aaagcatggc catataaaca agacgagtgt
     6841 acacgacaca gaatattttc tccgcgcaca tctcccatga ccaaagcaat actttctttt
     6901 tgcccgtcat aataaagata ttcacggtat gcaccgtaca aagttttaat agttccttct
     6961 gcaagtcgaa caatcatggt tactctttca attcattact aaaattcaaa tttcacgttc
     7021 gagcaatggg aaaactgcac cgataatcaa aaaaacagaa ccgacaatac ccaatatcat
     7081 cggtataaac cacttttcca aaaaactatc aattatcgct tcttcgggtt tatcacgttt
     7141 gtataacaca gatactgtct gatgtatttc aaaacccggg ctggatcggt aaagagaact
     7201 ttcatgttga taagatttgc cataggcgaa gtattcgacc accggtgcat atgtggtgtt
     7261 tttgccgggt ctgcgtgatt cgcgaaatct taccacggtt ccttcgactt gatcgcattc
     7321 ggcgataaaa ttttgttttc gtcgtactag ataatatgcg atgcccagta aagtcaggct
     7381 gacaaattga aaaatgtatg ctatcatatt tttttagagt taataatcgt aagtttccgg
     7441 tttacttttg gatgcaaatg cgataacgtg accatccgga tcagctacat atcctacggt
     7501 gtctccccaa tctctttcag cgatggggtc aataaacgca gcgccatgtc gtaaagcatt
     7561 ctcaagttgc tcgtgagcat tttccacaaa aagataaagt tcgcaacgcg gtattcccac
     7621 tccggtatca ggatgcggca tacgatctcc gatgattttg gcaatgccgt gcgtgggcat
     7681 taacccaagt ttgcatgtat ccgtgagcaa aaactcggtc atgcccggta catcaagcac
     7741 agcttcgcgt tgaaggatat tacggtaaaa atcgcgactt ttcgtctgat ctttgacata
     7801 cagaataatc tcaagtgacg ttgtataaga tttcatactt tatgttttaa taatatgtaa
     7861 ttttgcgaag taatttattt gtacccaaga acctctttac gatgtttttg agcccaggcc
     7921 acgatgccgt caatgaccgg gtcaagtgat tggccatgag gggttaaccc ataaacaacg
     7981 acggaagacg ttttaccatt tgcagatcta aatactaatt tattcatctc aagatatttc
     8041 agttcgcgcg tcaatatacg cggcgtgatc ggatggagat cacgttgaag ttcgttaaac
     8101 cttttttgct tttcgcataa gctggctaaa atagggccac gccatcgtcc gccgatcaca
     8161 tccaaaacat cctgtacatc ccttacacgc aaacgtttct ttttatttcg catagtaata
     8221 tcctaaagga tagtattttt tccggttgga ctcttgtcca aagtaactaa cgtgattatt
     8281 ttttgaaagg aaataaaaag gagcaaacat gaaaatatcg gttatcggcg catcttccgg
     8341 ggtcggatta caatgcgtgc agcgggcgct tcagcgaggt cacgccgtga ctacgctctc
     8401 ccgctcaccg ttacccgtaa atccatcgaa tgatcttttg attatacgcg gaagtgcttc
     8461 aaacaaagag gatattaaaa aaacaataaa agacgcagat gccgtactt
//
LOCUS       DAOQNQ010000127         8417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3098_length_8417_cov_7.166109, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000127 DAOQNQ010000000
VERSION     DAOQNQ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8417
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3098_length_8417_cov_7.166109"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PK754_08965"
     CDS             <1..298
                     /locus_tag="PK754_08965"
                     /inference="COORDINATES: protein motif:HMM:NF015415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HNB09675.1"
                     /translation="KQSFEWPIAEIAVALAIENNICRSARIVLGAAAPIPWRCTDAEN
                     LLIGQTITKESAACAAKKALSNATPLSKNAYKISIFETVIRRALCFAVGIDPLR"
     gene            339..767
                     /locus_tag="PK754_08970"
     CDS             339..767
                     /locus_tag="PK754_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09676.1"
                     /translation="MAKNIFSVIGGIITVIVMVTIVQNVGATIYPPPADFNPNSVETL
                     EAHLRTLSLGAFLFAVAGMALGSFMGGLVASWIAPENGYRNAIIVAAFYVVFGVIGML
                     MISHPIQYWVANFITYLPCALLGHITLQRAVNKKNQELNF"
     gene            773..982
                     /locus_tag="PK754_08975"
     CDS             773..982
                     /locus_tag="PK754_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09677.1"
                     /translation="MNARSDCKHIRTKTAYVTEPDHHDAWRTDASATAQYWCLRTMLT
                     SGPDGSYVAPETCQHGRSCFEDVTL"
     gene            complement(1040..1786)
                     /locus_tag="PK754_08980"
     CDS             complement(1040..1786)
                     /locus_tag="PK754_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09678.1"
                     /translation="MRKTWIIITIAILACSPESGSPPNESPLVTRILAPAVVGLTDTV
                     KIYTYDADESTLQIEAKIFDGDGQVLSDVWKTNLHDDGLAGDMKASEGNYIGVIKSQT
                     LLDHNSGFFELQVIVTEAKSGATDKQSVFIEQNPNRTHPPEVILVTAPDTVNPSLVSE
                     FLITVTVSDPDGLGDIASVTRTTPSNLVLALRDDGVNGDATAHDGVYSERVSVNPSPP
                     AGSYTFTFKATDRIGLQSAAIPKTIVIISN"
     gene            1942..2376
                     /locus_tag="PK754_08985"
     CDS             1942..2376
                     /locus_tag="PK754_08985"
                     /inference="COORDINATES: protein motif:HMM:NF022214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HNB09679.1"
                     /translation="MEHTIKYPHRIGGWLWLLGLFLLINPIRILIALKQVSLPAANVL
                     ASNYLAYAEVTVTLCYLVYAIMVPIYFFRMDRRTPIMIMMLLLVNLVFVGLVGTSAQW
                     LSSDTNHNASSVRLAEFVIGAMMFIVWTLYLMRSERVKDTFN"
     gene            2574..3584
                     /locus_tag="PK754_08990"
     CDS             2574..3584
                     /locus_tag="PK754_08990"
                     /inference="COORDINATES: protein motif:HMM:NF022591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HNB09680.1"
                     /translation="MLKIITYLFVFTGISLSICSQDYYDTGDYTAMLTQAEETASADG
                     KKILETGRTMTLTNAEILPGSCWDYANAVFDRAGYVWGKRETVFKSVKAGPFVDIEKI
                     KNGDWLYYINHSYGDIEHSAIFVEWIDKENKLALMLSYGGENRQQPARYLPYDLRSVY
                     GITRANSKLGEDKPTPKPKTVTSTNVAPVTNAVSNTGNNTSVKANNSNATTNKNAAPS
                     ETQSTPAGGNTIDGMLVESLRIGTGVANMEITGVADTFSSSTTEKIYCWLRVNGGQGK
                     TVKVRWIHNGNSLGEVPLDIRSQSMRTYAFRTVSGRKGSWKVEIVDAQGRLLRATDFT
                     VN"
     gene            complement(3594..5165)
                     /locus_tag="PK754_08995"
     CDS             complement(3594..5165)
                     /locus_tag="PK754_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09681.1"
                     /translation="MKKMMLLFLAASLTVSAQITRQVLKPVKVDIFKNGNGFFVAEGN
                     LSMSNSEAQITDVPRTAFGTLWVGAMDKNIQISGLRAIEEKFANRRDPASLYEILISN
                     IGKQVIFRQSGEKAIEYTGILERVIGEVSQPVIVLRTTKGTMILNTWYAMHAEFPESF
                     QNTFSDTTKAMALRIKTNSAANAARFQMVYFQNQIGWTPSYKIDLVDDKNAQIILSGT
                     VVNDIQDIEKADVNLVVGYPHFEFANTLSPLTTPMPISDFLSALGYDARRPDFDNYGN
                     RMMAAQVARYEMSAGQADAGNFTPVGGTTVEDLFFYNLPDFSLKKNERAQINIFSATV
                     PYQHVYEVTLPNAFNIQEGYLYDKTPARKEPYEVWHSIRLENKTAMPWTTGAALTMQN
                     GKALGQDILQYAPAKSTTHVKITTSPDIYVTEEERETERKEDQKKKDGYSYDLVTISG
                     EINLNNYKDKDVKLKITRPITGKLREATAEPKSKVNVKSGSAINPETTLTWEVNLKKG
                     DARKITYTYEVYLRR"
     gene            complement(5243..5440)
                     /gene="rpsU"
                     /locus_tag="PK754_09000"
     CDS             complement(5243..5440)
                     /gene="rpsU"
                     /locus_tag="PK754_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNB09682.1"
                     /translation="MITVQVGENEPFDKALKRFKRKCLQSGLMRTLRDTAHYVKPSER
                     RKADRMRAIRKQKQIQAEEGN"
     gene            complement(5727..6407)
                     /locus_tag="PK754_09005"
     CDS             complement(5727..6407)
                     /locus_tag="PK754_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09683.1"
                     /translation="MRVNLLALFLFMYVGVTSVFSQEEEEHTKQKMAFISGSFSYQSS
                     TNANEELTIFLEDAKIKTFSVLPKLGLFINTNTAIGAGVGYTYEKLEASDYDGELTST
                     TPLFSIEPFMRSYRPLTDDASIFVDASAFYRFGTQTFDYYDEYDEDNYSEKTEKTSYG
                     VAISPGITYHLTENIAMELVVGKLHYTKTKNKPKGAPDDVAFKSSSYGLKLDLDQASL
                     GVVIYLTR"
     gene            complement(6550..7056)
                     /locus_tag="PK754_09010"
     CDS             complement(6550..7056)
                     /locus_tag="PK754_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09684.1"
                     /translation="MKKLVVLALLVMLATAVQTNAQMKAGSMRPALELNFGGLGLGYG
                     ASFEYGITDKISARASANISSYKTGGDEWSLIPIDAVGVYNVDDSKYAMLGVTWTTFS
                     VDPSIGKKESSSSFGVVAGGGYRYKLNDKISVNGEGKYRVITLESGAYKLSVAWYTVG
                     AGFSYALN"
     gene            7287..8177
                     /locus_tag="PK754_09015"
     CDS             7287..8177
                     /locus_tag="PK754_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNB09685.1"
                     /translation="MVFFILIYIIAPLLLSYSAVAQDSVSFVYTNNTNGYIEACDCGE
                     EPLGGLARRKFIFDNIRKNERAVIFVDAGDFLNQFGFSPQQDSMTIRLYELLSYDAVN
                     PGDNEFANGYDFFERIVSRSKLPFTSSTLWHDTRPIAQPYQIVTRENYKIGIIGYTPF
                     SAFRYFPKNKQPGVHSVNERERLNAVIKDIRSRVDILMVLSHAGSEEDKLILSDFPDI
                     DMIVGAHDQVEIEEPIVDKNRYIVQAGGNGTKVGKLTLYLKSGKMINHTNRFYTLSEN
                     ITEDAEIKKLITRFKKANKK"
BASE COUNT         2354 a         1869 c         1721 g         2473 t
ORIGIN      
        1 aaaacaatcg tttgaatggc cgatcgcgga aattgccgtt gcactcgcta tcgaaaacaa
       61 catctgccga tcggcgcgca tcgtgctggg cgccgcggct ccgataccct ggcgttgcac
      121 cgatgctgaa aaccttctca tcggtcagac aatcaccaaa gagtccgccg cgtgcgccgc
      181 gaaaaaagct ttatccaacg cgacgccgct atctaaaaat gcatacaaaa tttccatatt
      241 tgaaaccgtg atccgacgcg ctttgtgttt tgcggtcggt atcgatcctt tgcgataaac
      301 tgttttagtt tttacatcaa acaacgacga aaggttttat ggctaaaaat attttttccg
      361 ttatcggcgg tatcatcaca gtgatcgtaa tggtgactat cgttcaaaac gtcggtgcca
      421 cgatatatcc tccccccgcc gatttcaatc ctaacagcgt tgaaacgctg gaagcgcact
      481 tgcgaacact ttccttaggc gcatttcttt ttgcggtggc gggtatggcg ctgggttctt
      541 ttatgggcgg tctcgtagcg tcctggattg caccggaaaa cggttaccgt aatgcgatca
      601 ttgtcgctgc attttatgtg gttttcggtg tcatcggtat gctcatgatc agtcacccta
      661 ttcaatattg ggtcgccaat tttatcacct acctgccctg cgcattactt ggtcatatca
      721 ccttacagcg cgccgttaac aaaaaaaatc aggaacttaa tttttaatca aaatgaacgc
      781 acgatcagat tgtaaacata ttcgcactaa aaccgcttac gttacggaac cggatcatca
      841 tgacgcatgg cgaacggatg cttcagctac agcgcagtac tggtgtttgc gaacgatgtt
      901 gacttccggg ccggatggct cctacgtcgc gccggaaact tgccagcacg gaagaagttg
      961 ttttgaagat gtaacgcttt gatgtaagga tgtggatttt agatacgttt tgataaacta
     1021 tacggcagag gctgcgattt taatttgaaa taatgactat cgtttttggg atcgcggcgc
     1081 tttgaagacc gatccgatcg gtcgctttga atgtaaatgt ataactcccc gctggcggtg
     1141 aaggattgac cgaaaccctt tctgagtaaa caccatcatg tgccgtcgca tctccgttca
     1201 caccatcatc acgtaaggct aaaaccaaat tacttggcgt cgtacgcgtc acggatgcta
     1261 tatcacccaa accgtccgga tcggaaaccg taacggttat taaaaattca ctcaccagac
     1321 ttgggtttac cgtatcggga gccgtcacta agataacttc aggcggatgc gtgcgatttg
     1381 gattctgttc tataaacacc gactgcttgt ccgtcgctcc gctcttcgct tctgtcacga
     1441 tgacttgcaa ttcaaaaaaa ccgctgttgt gatccaaaag agtttgcgat ttgatcactc
     1501 cgatatagtt gccttcactg gccttcatat caccggcaag accatcgtca tgcaaattcg
     1561 ttttccacac atccgatagc acttggccgt caccatcaaa gattttagct tcaatctgta
     1621 gcgtgctctc atcggcatca taggtataaa tttttactgt atctgtcaac cctacgacgg
     1681 caggtgcgag tatacgcgtt accaatggcg attcattggg cggcgatcca ctttccggcg
     1741 aacacgctaa aatcgctata gttataatta tccacgtctt acgcatacgg ataagataat
     1801 ggaaattttt acggaatcaa aaattataat ccattctata aatgaaacca tacgcatgtc
     1861 gataaaaaat acgccgctac tgacctccgt cattgctccg ccaaaggcta cactgtactt
     1921 tgattgtgaa ggaggtgtct tatggagcat actatcaaat atccgcatcg tatcggagga
     1981 tggttatggt tattaggttt atttctgctg attaatccga ttcgtatatt gatcgcactc
     2041 aaacaagtgt cattacccgc tgcgaatgtc ttggcatcca actaccttgc gtacgcagaa
     2101 gtcaccgtta cgctctgcta tcttgtttat gcgattatgg tgccgattta ttttttccga
     2161 atggatcgtc gcaccccgat aatgatcatg atgcttttac tggtcaatct ggttttcgtc
     2221 ggtctggtcg gtacatcagc acagtggttg agttcagata caaatcataa tgcctcatcg
     2281 gtacgtttgg ccgaatttgt aataggcgcg atgatgttca tcgtatggac tttatatttg
     2341 atgaggtccg aaagagtgaa agacacgttt aattaggcat tcactcggct accgacgatt
     2401 tgagtaagcc tgtacatatc tctaaattgt aaattgcgaa tcacgccgat tcgtcctatc
     2461 ttacgtacgt cctaaagaca gacttatatc ttcctgtctg tattatttta cggttcacca
     2521 ttttctcatt ttttaaattt catttcatac accttaaacg caggaatagc ggtatgctta
     2581 aaattattac ctatcttttt gttttcacgg gtatttcgct ctccatctgt tcacaggatt
     2641 attacgacac gggcgattat acggcgatgc tgacccaagc ggaagaaact gcatcagcgg
     2701 atggaaaaaa aatattagag accggccgaa cgatgacatt gaccaatgcc gaaatactac
     2761 cgggcagttg ttgggattat gccaatgcgg tttttgatcg tgcgggttac gtgtggggaa
     2821 aacgcgaaac tgttttcaag tcggtcaaag ccggtccttt tgttgatatt gaaaaaatca
     2881 aaaacggaga ctggttgtat tacatcaatc attcttacgg ggatattgag cacagtgcta
     2941 tttttgtaga atggatagat aaagaaaaca aactggcttt gatgctgagt tacggcggcg
     3001 aaaaccggca acaacccgca cgttacctgc cttacgattt gcgcagtgtc tatggtatca
     3061 cacgcgctaa ctccaaatta ggcgaagaca aaccgacccc taaaccaaaa accgtgacaa
     3121 gtaccaatgt tgccccggtt acaaatgcag tttcgaatac aggcaataat acgtctgtaa
     3181 aagccaataa cagcaatgca actactaata aaaatgcagc accatctgaa acccaatcaa
     3241 cgcctgccgg cggcaatacg attgacggta tgctggtgga atctttgcga atcggaaccg
     3301 gcgtagcgaa tatggaaatt accggtgtgg cagatacttt ctcttcctcg actacagaaa
     3361 aaatttattg ctggctgcgc gtcaacggag gccagggaaa aaccgtcaaa gtgcgatgga
     3421 ttcacaatgg aaactccctc ggtgaagtgc ccttggacat ccgatcacaa tccatgcgca
     3481 cttatgcatt tcgtaccgtc agcggacgca aaggaagctg gaaggttgaa attgtggacg
     3541 cacaagggcg attgctccgc gcgacggact ttaccgttaa ctgatttttt aaatcatcgt
     3601 ctgagataca cttcgtaagt atacgtgatc tttcgggcgt cgcctttttt cagattaacc
     3661 tcccacgtca atgttgtttc gggattgatc gcacttcctg acttgacgtt gactttactt
     3721 ttgggttccg ccgttgcttc gcgcagttta ccggttatgg ggcgtgttat tttgagtttg
     3781 acgtctttat ctttgtagtt gttcagattg atctcgccgg atatggtaac gagatcatac
     3841 gaatacccat ccttcttttt ctgatcttct ttgcgctctg tttcacgttc ctcttccgta
     3901 acatagatat cgggcgaggt cgttattttg acgtgggttg ttgatttggc cggtgcgtac
     3961 tgcaaaatat cctggcccaa agctttgccg ttttgcatgg tcagcgcggc accggtcgtc
     4021 cacggcatag ccgttttatt ttctaaccga atggagtgcc acacttcgta aggctctttg
     4081 cgcgccgggg ttttatcgta gagatacccc tcttgtatat taaatgcatt aggaagcgtt
     4141 acttcataga catgttgata aggcaccgta gccgagaaaa tattgatctg cgcgcgctca
     4201 ttttttttca atgaaaaatc aggtaggtta taaaagaaaa ggtcttcgac agtcgtacct
     4261 ccgacaggtg taaaattgcc cgcatcggct tgtcctgcgc tcatctcata acgagcaact
     4321 tgcgcggcca tcatccgatt accataatta tcaaaatcag ggcgacgtgc atcatatcct
     4381 aacgcggaca ggaaatcact gatcggcatc ggtgtggtaa gcggtgataa tgtgttggca
     4441 aattcaaaat gcggataacc tacgaccaga ttaacatcgg ctttttcaat atcctggata
     4501 tcattgacta ccgtgccgga taagatgatt tgcgcgtttt tatcatcaac gagatctatc
     4561 ttgtaactgg gcgtccaccc gatctggttt tgaaaataga ccatttgaaa tcgtgcggca
     4621 tttgctgcac tattggtctt tatgcgcagc gccatcgctt tggtcgtatc ggaaaacgta
     4681 ttctgaaaag actccggaaa ctcggcatgc atcgcatacc acgtatttaa tatcatcgtc
     4741 cctttggtcg tacgcaatac aataaccggt tgcgatactt cgcctatgac acgctctaaa
     4801 atccctgtgt attcaattgc cttttccccg ctttgacgaa atatcacctg tttgccgata
     4861 ttggagatta atatttcata caatgaagcc ggatctcggc gattagcaaa tttctcttcg
     4921 atcgcacgta aacccgatat ttgaatgttc ttgtccatag cgcctaccca cagcgttcca
     4981 aacgcggtcc ggggcacatc tgtgatttgc gcttcactgt tactcatact gaggtttcct
     5041 tcggcgacaa aaaaaccgtt cccgttttta aaaatatcca ctttgacagg cttcagtacc
     5101 tgacgtgtga tttgagccga aacggtaagg ctcgccgcta gaaacaataa catcattttt
     5161 ttcatgacca ttcctttcgt catataaaaa agaaaaacca tcccgaaaag ggatggtttt
     5221 tgaaaactac ttcgcaaaca gactagttgc cttcttcggc ctgaatctgt ttttgtttac
     5281 ggatagcgcg catgcgatcg gctttgcggc gttcgctcgg cttcacatag tgcgctgtat
     5341 cgcgcaatgt gcgcataagg ccggattgta aacatttacg cttaaaacgc ttgagcgctt
     5401 tatcaaaagg ttcgttttcg ccgacttgga cggtgatcat gaggtatcct tcggttttgt
     5461 tttatttata aactattgta aaaataaact ttaaaagaac gcatgaataa ctattatcag
     5521 ctttctgaca atacgcgcta aatttagttt ttttaacggc gaaagcaagt aaaaaatatt
     5581 ttgttttgcg ctacgtctat ttccatccaa gctttctttc ctaaaaaaca aatctctgaa
     5641 ggctacccct caattacgca ttaagttcac tacttctgat tataatcgga caaactacag
     5701 tttctgtaat gccatgcaat ttttggttaa cgtgtcaaat aaattacgac accgagtgag
     5761 gcttgatcta agtctaattt gagcccatac gaagagcttt taaatgcaac atcatccggt
     5821 gcgcctttag gtttattttt agtttttgtg taatgcaatt ttcctaccac caactccatt
     5881 gcaatgtttt cagttagatg gtaagtgatc ccagggctta tcgcaacacc atacgacgtt
     5941 ttttcagttt tttccgaata attatcctca tcatactcgt cataataatc gaacgtttgt
     6001 gtaccaaacc ggtaaaacgc tgatgcatct acaaaaatgg aagcatcatc agtcaaaggt
     6061 ctgtaactgc gcataaacgg ctctatcgaa aaaagcggtg ttgttgaagt aagctcacca
     6121 tcgtaatcac tggcttccaa tttttcgtac gtataaccca cacctgcacc tatagccgta
     6181 tttgtattga taaatagacc aagtttagga agtacactaa acgttttgat ttttgcatct
     6241 tccaaaaaga tcgtcaattc ttcattggcg tttgtggatg attggtagct aaaagaaccg
     6301 gaaatgaaag ccattttttg ctttgtatgt tcttcttcct cctgcgaaaa aacactagta
     6361 actcctacat acataaacag aaaaagtgct agtaaattga cacgcataga ccctccctaa
     6421 taaagattaa taagatattt actacgaaaa acgactggag aaggtatgag tatcatataa
     6481 attaaacaaa caaaaaaagc cccgctgcca acagtcagcg aggcttggaa tacctgaagt
     6541 ctggataagt taattcaaag cgtaagagaa gcctgcgccg acagtgtacc atgctacaga
     6601 aagtttataa gcacctgatt caagtgtgat cacacggtac tttccttctc cgttgactga
     6661 aattttatca ttgagtttat agcgataacc tccaccagca actacgccga acgacgaaga
     6721 actttccttt tttccaatgc tcggatcaac tgagaaagtt gtccacgtaa cgcccagcat
     6781 ggcatactta ctatcatcaa cattataaac accaaccgca tcaatgggga tcaatgacca
     6841 ttcatcaccg ccggttttgt aacttgaaat atttgcgctg gcgcgtgcac tgattttgtc
     6901 tgtgatacca tattcgaagc tggcaccata accaagacca agacctccaa aattcaactc
     6961 caacgcggga cgcatacttc cggctttcat ttgtgcattg gtttgaaccg ctgtggctag
     7021 cataactaac aaagcgagaa caactaattt tttcatgtgt aactcctaca tttagtttat
     7081 tataaaaaag agaaccgatt ttttttagta actatgtgat ttttaaaatg tgaagaaccg
     7141 ataacactta tcagatgtat caagtcttat gccaaaaaat gatttacttt taagtgttat
     7201 aaattataga tttcaattgt aacattatat taagcgtcgc aaaaatgtac caatacactt
     7261 gtatcagtcc gtcgcgctat tgcgacatgg ttttttttat cctgatttat atcatcgcac
     7321 ccttgctatt atcttatagc gcagtggctc aggattctgt ttcttttgtt tataccaata
     7381 ataccaacgg atatattgaa gcatgcgatt gcggagagga acctttgggt gggcttgcac
     7441 gaagaaagtt tatattcgac aacataagga aaaacgagag agcagttatt tttgtagatg
     7501 ccggagattt tttaaatcaa tttggttttt ctcctcagca ggattctatg acgatacgat
     7561 tatacgaatt gctttcctat gatgctgtaa atccgggtga taatgagttt gccaatggat
     7621 acgatttttt tgagcgtatc gtctcccgat ctaagttacc attcacttca tctacacttt
     7681 ggcatgatac ccgacccatt gcccaacctt atcagatcgt aactagagag aactataaga
     7741 tcggaatcat cggttataca ccattttccg cgtttcgata ttttccgaaa aacaagcaac
     7801 ccggagtaca ctctgtcaat gaaagagaac gtttgaatgc agtaataaaa gatattcgct
     7861 caagagtgga tattcttatg gttctgtctc atgccggttc agaagaagac aagttgattt
     7921 tatcggactt tccggatatt gatatgatcg tcggtgcaca cgatcaggtt gaaatcgaag
     7981 aacctatcgt ggacaaaaat agatacatcg tacaagccgg tggcaatggc actaaagtcg
     8041 gaaagcttac attgtatttg aaatccggta aaatgataaa ccacactaac cggttttaca
     8101 cattgagtga aaatatcacg gaagatgcgg agataaaaaa attgattaca cgttttaaga
     8161 aagcgaataa gaagtaattt aaaaaaaaga ttgcctcaat ccgttacaca cacatctact
     8221 catcaaacaa ttcatcaaat tttcgggtcc aatgatgaat agatccatct aaactttttt
     8281 gaggcaatct ttattcgtta taacatataa aatataaaac tacatttcta tttttacgcc
     8341 catcgtcgga gcgacacgaa atccgcgttt gtccaactta ccgttttgat actgaaaaaa
     8401 ctcctcgtta acattga
//
LOCUS       DAOQNQ010000128         8356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3142_length_8356_cov_6.582821, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000128 DAOQNQ010000000
VERSION     DAOQNQ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8356
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3142_length_8356_cov_6.582821"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..1318
                     /locus_tag="PK754_09020"
     CDS             182..1318
                     /locus_tag="PK754_09020"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNB09686.1"
                     /translation="MQISLTMVSFLLLISTAHGIFFTILTAGKLRRSSSARILFVIFM
                     LLILANAEYLFIASGAYTTFSHFFGISNGFMLLFGPLMWLYSRSLTDAEFEISKIQSL
                     HCVPYLLFCGVSWPLWFLDHRAKVAFIEVFISGHLPLRVIDYVIFVLQVIHFTAYMLL
                     SLRSVRIEKQQLLDRMYQEDITGRLKWIKFLIGFFCAYLILVSGLVIQVFYSGVFSPD
                     ANYAYTLALSAIMYFAMYYGFNNPDAVTGGFTQKYKNVVLSSEEMSGYTEAIRSAFDD
                     EKMYLQPDLKLADVAKKINASPHLVSQLINQHYGHNFFDHINTLRIAEFKRRLAQPDV
                     NIMTLLGIALESGFNSKSTFNAVFKKFEGITPLEYKKSLNKPQN"
     gene            1409..1924
                     /locus_tag="PK754_09025"
     CDS             1409..1924
                     /locus_tag="PK754_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09687.1"
                     /translation="MKILLIIGAVVVSGLAIYAVYMGVFVSITIREQRIGPYHFIYKE
                     MHGTDAREVGKITTELRLLLTNNGFTNIAPLDIYFPDQKPNHIGFMADDARVEAIITT
                     DSLLKYRNIPEQRCMTTGFPYRNPMSYLVGYIKVDAALSKYRETHGYKKTEAGTIHQD
                     ENILYFQPIIP"
     gene            2021..2539
                     /locus_tag="PK754_09030"
     CDS             2021..2539
                     /locus_tag="PK754_09030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008311419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB09688.1"
                     /translation="MTDHQREHITLRKATSADSIILRFWDEQPHVIESDPNDDWHWEE
                     ELQREPSWREQLIAELDGKPIGFIQIIDPQREETQYWGAIGSGYRAIDIWIGEAGNLS
                     KGYGTAMMHQAIEHCFAEEDVHTVIIDPLANNARAHRFYERLGFRFVREQRFGEDDSF
                     VYELKRGDWMKR"
     gene            2669..5035
                     /locus_tag="PK754_09035"
     CDS             2669..5035
                     /locus_tag="PK754_09035"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB09689.1"
                     /translation="MLTNYLKMAIRSFVRNRILSTINIFGLAVGIACCSVIFNYVYDE
                     LTYDGYHVNSEHLYRIEVGNWACTAVGVGPYIKRTFSDVKEITRFYRMPRTIMANGEK
                     RFIEKRGFYADASVFDLFSFDLIQGDPSTALTAPNTIVITTELAHKYFGTEDPVGRSV
                     QIGADADEYKITGVMKAVPAKSHFKFDFLVSLNTLQFPLDNARVQWANTTSYTFALLT
                     ANVNIGTLENQIASAISEKQKAAFGSADPVSVVLQPVYKIHMYSKCEKEIEPSNDINN
                     ILIISSMALFILITAIFNFVNLTTAYFIRRTKEVGLRKTFGADKYQIVRQFLMESILI
                     TLIASVFSLVVMDVLLYFLNSTSNFHLNSSLWSSPLTIVGFLFFSLFVGIIAGLYPSF
                     VLSRLQPAHIIKQNGATSKAFSINVLRKTLLVLQFVISAILIIGTGIIYNQLSFLQNK
                     NLGLEKERVVILPISRISEQNYNTLKFLLGQSSKILGVSGSISIPGERVIYEGIIRQG
                     TVEELGVRLIVADYDFTETYGMTLVEGRNFSNQMSSDSSGAFIINEKAKNLLEYKEVI
                     GKTLEVSGLKQKGAVVGVVKDFNFTSLRSEIQPLVIFLNRSTMRYNYISIKFIPNSTT
                     SAIQQIETVWMKVFPNIPFEYYFLDDAFESLYQSEINLRAIAGIFAILAMVIGALGLL
                     GLVSLSVGQRTKEIGIRKVLGATVFEIVALISKDFLVYVILANLIAWPIIYFGMKRWL
                     DNFAYRIEIDFMIFAEAMLLSLGITIVTVAFQALKAAITPPVETLKCE"
     gene            complement(5096..7006)
                     /gene="acs"
                     /locus_tag="PK754_09040"
     CDS             complement(5096..7006)
                     /gene="acs"
                     /locus_tag="PK754_09040"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNB09690.1"
                     /translation="MSTDNNKPISNQEMYHPSKDVIANAHIKDYETLYRRSVTDREGF
                     WAEEAGKLEWYKSWDRVLDDSKKPFYKWFTGGQTNIILNAIDRHLRDWRRNKLAIIWE
                     GEPGDVRTYSYHALNREVSKFANVLKSMGVRKGEIVTIYMPQIPELVFAMLACAKIGA
                     AHSVVYGGFSVEALAARIEDAKSRVLVTADGGWRRGKINDLKTIADEAIKRSPTIEVC
                     ICVKRTGHEVRMENDRDFWYHDLMSLPIANPKCPTEVMDAEDMLFILYTSGTTGKPKG
                     LVHTHGGYSVYTSTTHRYVFDIKDEDRWWCAADPGWITGHSYIVYAPLINGATVMMYE
                     GAPNHPYPNRWWQIIEKYGISILYTSPTAIRGLMRFGEQWPKRHDLSTLRLLGSVGEP
                     INPEAWKWYYRNIGQERCPIMDTWWQTETGGFMVTPLPVIPLKPGSATKPFFGNEIAV
                     VDDHGKEVAAGEEGKLIIKSPWPGMARTILGDPARFVETYWKEFEKQGWYKAGDSARL
                     DEDGYLWVIGRIDDVIKVSGYRLGTAEIESALVSHPAVSEAAAIGLPHEVKGQAIYSY
                     VILRAGQTPSKELEETLKEHVAKEIGPIARPESVTFLDVLPKTRSGKIMRRVLKARAL
                     GLNEGDVSTLEE"
     gene            7379..8266
                     /locus_tag="PK754_09045"
     CDS             7379..8266
                     /locus_tag="PK754_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09691.1"
                     /translation="MTKKIITLYLSILTLSSCAHQKRVLLHVQGLSNENYQTRSFQIN
                     GSSGVPIETGISVEQGDFVFIEVLGAIHLGSDGGLTNGDGISGYSKQNKYNYKHGSVV
                     TLVAGKDHGMTTISLFRHPIYYQLVDFAKLTDGNYLPCLYFFSDTSGNISFEINDVDY
                     SNNSGEFTVNVYHLKKSGDLGRNSYNKCPMKEPENGKDCEGNTWVKEGIKGWFYHGLF
                     NNSYRGTGKNSGCQCVYTKGDLVVSHPDQGTFDYGFWEYQGDIDDVQARHLHLIWDVL
                     PHDLWIGKKTYNDPHIICK"
BASE COUNT         2234 a         1702 c         1897 g         2523 t
ORIGIN      
        1 attggaaagc ggcgcatttc gtacgctcgt caaagtaccc gaaaatgagg aatgggtgct
       61 gcgcgaagcg ctgattcgta ccgtcgtacc gcccagactg cgcccctatc tgggcaaagg
      121 catcgggtaa ctgaaataca aatattgttt cgcaatgtat aaccaactta tgtgctgacg
      181 tatgcaaatt tcgctcacta tggtaagttt tttacttttg atcagcacgg cacatggtat
      241 tttttttacg atactgacgg cgggcaagtt gcggcgctct agctcggcgc gtattctttt
      301 tgtcatattc atgctgttga tactggcgaa tgccgaatat ctttttatcg cttcaggcgc
      361 gtacacgaca ttttctcatt tctttggcat ttcaaacgga ttcatgcttc tttttgggcc
      421 actgatgtgg ttatacagcc gcagcctcac ggatgcggag ttcgagatat caaaaatcca
      481 gtcgcttcat tgcgtgcctt atttattgtt ttgcggagta tcatggccgc tttggttttt
      541 agatcaccgg gccaaagttg catttatcga ggtttttatt tcgggccact tgccgttacg
      601 ggtcatagac tacgtgatct tcgttttaca ggtgattcat tttacggcgt atatgctttt
      661 gtctttgcgt tctgttcgca tagaaaagca acaactgctt gatcgcatgt atcaggagga
      721 tattaccgga cgattaaaat ggattaaatt cctcatcggc tttttttgcg cgtatctgat
      781 actggtttcg gggttggtga tacaggtgtt ctatagcggg gtgttttcgc cggatgctaa
      841 ttatgcttac acgttggctt tatcggcgat catgtatttt gcgatgtatt acggatttaa
      901 taatcccgat gcggttaccg gtggatttac ccaaaaatac aaaaacgtcg ttctaagttc
      961 agaggaaatg tccggatata cggaagctat tcgcagcgca tttgatgatg aaaaaatgta
     1021 tttgcaaccc gatttaaaat tagccgatgt tgccaaaaaa ataaatgcct cgccgcatct
     1081 tgtatcgcag ctgatcaatc agcactacgg gcataatttt tttgatcata tcaatacact
     1141 acgtatcgct gagttcaaac gtcgtttggc acaaccggat gtaaacataa tgacattgtt
     1201 aggcatagcc ctggaatccg gtttcaacag taaatctaca ttcaatgccg tattcaaaaa
     1261 atttgaagga attaccccgc tagagtataa aaaaagcctg aataaacctc aaaattgagt
     1321 ccgactttat aaagttgagc gattcccgcc gataaagaga ttattttacc cgatcatttt
     1381 cttttttaat caaacattaa agggcgttat gaagatttta ctcattatcg gcgcggttgt
     1441 cgtatcggga ttggcgattt atgcagttta tatgggcgtt tttgtatcca ttaccatacg
     1501 tgagcagcgt attggtccgt atcattttat ttacaaagag atgcacggaa cggatgccag
     1561 agaggtcgga aagataacga ccgaattacg gcttttattg accaataatg gttttacaaa
     1621 tattgcaccg ctcgatattt attttcccga ccagaagccc aatcacattg gtttcatggc
     1681 cgacgatgcg cgcgtggagg cgattatcac cacagactcg cttttaaaat accgaaatat
     1741 tccggaacag cgctgtatga cgaccggttt tccttaccga aatccgatgt cgtatttggt
     1801 cggatacatc aaagtggatg ccgctttgag caaataccgt gaaacccacg gttacaaaaa
     1861 aacagaggcg ggtaccatcc atcaggatga aaacatactg tatttccaac cgataattcc
     1921 gtaacttcac atatccgatt tggtttattt tttaattcag gtgtggttgc tgcacagtca
     1981 gcgtccacgc cattcgttat ttactttatc gtccttagct atgacggatc atcaacgaga
     2041 acacatcacg ctgagaaaag cgacttctgc ggattcgatt attctccggt tttgggatga
     2101 acagccgcac gtcatagagt ccgatcccaa cgacgattgg cactgggaag aggaactgca
     2161 gcgcgaaccg tcgtggcggg aacagctcat cgccgagctt gacgggaaac ctatcggctt
     2221 tatacagatc attgatccgc aacgggaaga aacccaatac tggggtgcga tcgggagtgg
     2281 ctatcgtgcg attgatatat ggatcggtga agccggaaat ctgagcaaag gttatggaac
     2341 tgcgatgatg caccaggcca tcgaacactg ttttgcagaa gaggatgtgc ataccgtgat
     2401 catagacccg ctggcgaata acgcccgggc gcaccggttt tacgaacgtc tgggatttcg
     2461 ttttgtacgc gaacaacgat tcggagaaga tgacagcttt gtgtatgaac tgaagcgggg
     2521 agattggatg aaacgatagg gtggtggtta tttaaagccg tgatcttcta tttaaatttg
     2581 aacctgatgg cgatatcaag aaataggtgg cgttttgtaa agcaatagtg tcgtttctag
     2641 atgtgcttta accgatgtga ggaaaatcat gctgacaaac taccttaaaa tggctatccg
     2701 ttcttttgtg agaaatagaa ttctctcaac gattaatatc tttggattag ccgttggtat
     2761 tgcatgttgc tcggtaattt ttaactatgt ttatgacgaa ttaacgtacg atggttatca
     2821 tgttaattcg gaacatcttt atcgtattga ggttggtaat tgggcttgta ccgcggtggg
     2881 ggttggccct tatatcaaac gaactttttc tgacgtaaaa gagatcacga gattctaccg
     2941 aatgccacga acgataatgg ctaacggaga aaaacgattt attgaaaaga gaggatttta
     3001 tgctgatgcc tcggtttttg atttgtttag ttttgatcta atccaaggag atccgtcaac
     3061 cgcgttaaca gcacccaata cgatcgtcat tacgactgag ttggctcaca aatattttgg
     3121 aacagaagat ccggtgggtc ggagtgtaca gataggcgcc gatgccgatg aatataaaat
     3181 cacgggagtt atgaaagcgg ttccggcaaa atctcatttt aagtttgatt ttttagtttc
     3241 actcaatact ctccaatttc cattggataa tgctcgtgtg caatgggcta atacgacgtc
     3301 atatactttt gcactactta cagcaaacgt aaatatcggt acacttgaaa atcagattgc
     3361 atcagcaata tcggaaaagc aaaaggctgc gtttggatct gctgacccgg tttcagttgt
     3421 gctgcaaccg gtttacaaga ttcatatgta ttccaagtgc gaaaaagaaa tcgaaccaag
     3481 caatgatatc aacaatatcc tcataatttc cagtatggca ctatttatac taatcacggc
     3541 aatttttaat tttgttaatt taacaacagc ctattttatc agacgtacca aagaggttgg
     3601 tttgcgtaaa acatttggcg ctgataaata tcagatcgtc aggcagtttt taatggagtc
     3661 cattctgatt actttgattg cgtccgtatt ttctctggtg gtgatggatg tcttattgta
     3721 ttttttaaat agcacttcaa attttcatct gaatagttct ttgtggagta gcccactaac
     3781 cattgtaggg ttcttatttt ttagtctatt tgttggtatt attgccggat tatatccatc
     3841 atttgtttta tcacgacttc agcctgctca catcatcaaa caaaatggag caacttcaaa
     3901 agcctttagc atcaacgtgc ttagaaaaac cctacttgta ttacaattcg ttatttccgc
     3961 aattcttatt attgggactg gtatcattta taatcagctt tcattcttac aaaataaaaa
     4021 ccttggtttg gaaaaggaac gggttgtcat tctgccgatt agtcgtatat ctgagcagaa
     4081 ttacaacact ttaaaatttt tattgggaca gtcatctaaa attcttggcg tttccggttc
     4141 aataagtatt cctggtgaac gagttattta tgagggcata atacgacaag gaacggtaga
     4201 agaactaggc gtgcgcctca ttgttgctga ttatgatttt actgaaactt atggtatgac
     4261 actagttgaa ggacgtaatt tttcgaatca aatgtcaagt gattcgtcag gtgcttttat
     4321 aattaatgaa aaagccaaaa atctcctaga atacaaagaa gtaataggaa aaactttaga
     4381 agtttccggt ttaaagcaaa aaggtgcggt agtcggagtc gtaaaagact ttaattttac
     4441 gagcctacga tctgagatac agcctctagt gatctttctt aatcgcagca ctatgagata
     4501 caattatata tcgattaaat ttatcccgaa cagtacgacc tcggctattc agcaaattga
     4561 gaccgtttgg atgaaggtat ttccaaacat tccattcgaa tattatttcc tggatgacgc
     4621 ttttgaaagt ttatatcaat cagaaatcaa cctcagagcg attgcgggca tctttgctat
     4681 attggctatg gtgatcgggg cgctcggatt attggggttg gtctcccttt cagtcggaca
     4741 acgaacgaaa gagataggta tccgtaaggt tttaggagca acagtctttg agatcgtagc
     4801 actaatatcg aaggattttc tagtctatgt tattttagct aacctgatcg cttggcctat
     4861 aatatatttt ggaatgaaac gatggttaga taattttgca tatcggatcg aaatcgattt
     4921 catgatcttc gcagaggcta tgttgttgtc tctcggcata acaattgtta ctgtggcttt
     4981 ccaagcctta aaagctgcaa taacaccgcc ggtagagaca ttaaagtgcg aatgaaattt
     5041 aaatttataa tttcctaccc caaaacttta tcgccatgca ctaaaccgtt ttcaattatt
     5101 cctctaacgt tgatacatcg ccttcattca agcccaaagc gcgggctttg agtacgcggc
     5161 gcatgatctt gccgctgcgc gttttaggca gcacatccag aaacgtaacg ctctccggcc
     5221 gcgcgatcgg accgatttct ttggcgacat gttctttgag tgtctcttcg agttctttgc
     5281 tcggtgtctg tccggcgcgt aggatcacat agctgtatat ggcctggcct ttgacttcat
     5341 gcggaagccc gatggctgcg gcttccgaga cagccggatg gcttaccaac gcgctttcaa
     5401 tttcggccgt tccgaggcga tatccactga ctttgatcac gtcgtcaatg cgtccgatca
     5461 cccaaagata gccgtcttcg tcgagacggg ccgaatcgcc cgctttgtac caaccttgtt
     5521 tttcaaattc tttccaatac gtttccacaa agcgtgcggg atctccgagg atcgtacgtg
     5581 ccatacccgg ccacggtgat ttgatgatca gtttaccttc ttcgcctgcc gcgacttctt
     5641 tgccgtgatc atccacgacg gcgatttcat ttccaaaaaa gggttttgtt gcggagccgg
     5701 gtttgagcgg aattaccggg agcggtgtta ccataaagcc gccggtctct gtctgccacc
     5761 acgtgtccat gatcgggcag cgctcttgtc cgatattacg ataataccat ttccacgctt
     5821 cgggattgat cggctcgccg accgatccca acagccggag cgtactcagg tcgtgacgtt
     5881 tgggccattg ttcgccaaac cgcatcagac cgcggatcgc cgtcggtgaa gtgtaaagaa
     5941 tgctgatgcc gtatttttcg atgatctgcc accagcggtt ggggtacggg tgattgggtg
     6001 cgccttcgta catcatgacc gtcgcaccgt tgatcagcgg tgcgtagacg atgtagctgt
     6061 gacccgtgat ccatccggga tcggcggcgc accaccagcg gtcttcgtct ttgatatcaa
     6121 aaacgtaccg gtgggtcgtg gatgtatata cggagtagcc gccgtgcgta tgcaccaaac
     6181 ctttgggttt cccggtcgta ccggatgtgt aaaggatgaa aagcatgtct tcggcatcca
     6241 tgacttcggt agggcacttg ggattggcga tgggcaaact catcaggtcg tggtaccaga
     6301 aatcgcgatc gttttccatg cgcacttcat gaccggtgcg cttgacacag atgcatacct
     6361 cgatcgtggg ggaacgtttg atggcttcgt cggcgatggt tttgaggtcg ttgatttttc
     6421 cgcgccgcca tccgccgtcg gccgtaacga gtacgcggct cttggcatct tcgatccgag
     6481 cggccaatgc ttccacgcta aagccgccat acaccacgct gtgcgcagcg ccgattttgg
     6541 cgcatgccaa catagcgaaa acaagttccg gtatttgcgg catatagatc gtcacgattt
     6601 cgcctttgcg tacgcccatg cttttgagga cgttggcaaa cttggatact tcgcgattga
     6661 gtgcatgata ggaataggtt cgcacatcgc cgggttcgcc ttcccagatg atggctaatt
     6721 tatttcggcg ccagtctctc aaatgccgat caatcgcgtt gagaatgata ttggtctgtc
     6781 cgccggtaaa ccatttgtaa aacggttttt tactgtcatc gagtacgcgg tcccacgatt
     6841 tataccactc gagttttccg gcctcttcgg cccaaaaacc ttcgcggtcc gtgacggaac
     6901 gtcggtacag cgtttcataa tctttgatat gcgcattggc gatgacatcc ttggaaggat
     6961 ggtacatttc ctgattggat ataggtttat tgttgtcggt actcataatg cttctcctgt
     7021 ggacttgctc gtgatggggt tcaataaatg aatgctataa atttaacggt tcttattacg
     7081 aatccaagtt ttttggataa aaaatttggc gaaactatat gagaaagtta tgagccgaaa
     7141 tcctttgcgg aatggagata actaacggat ttcagtgtat acaaaaaaga tgcagggaaa
     7201 aattttacag ttaatctcac aaaaaattga actatttttt gtataaagaa attgccaatt
     7261 tatatataac gaaccgattt tttatatcat ccttttttta caaacatgat ttgcaatcca
     7321 gagattttaa atttttgaca taactgtctc cttataatat acattcaacg aaacctgcat
     7381 gacgaaaaag atcattactc tctatttgtc tattttgaca ctttcatctt gtgctcatca
     7441 aaaaagagta cttttacatg tgcaaggatt gtccaatgaa aactaccaaa cgcgctcttt
     7501 tcaaataaat ggcagttctg gcgtaccgat tgaaacgggt atttcggttg agcagggaga
     7561 ttttgtcttc attgaagttt taggtgcgat acatcttggc tcggacggtg gccttaccaa
     7621 tggtgacggc atttcaggat actcaaagca aaataagtat aactacaaac acggttcggt
     7681 ggttacattg gttgccggaa aagatcatgg catgacaacc atttcactgt ttcgccatcc
     7741 gatttattat caattggtag attttgcaaa gctgacggac ggtaactatt taccgtgtct
     7801 gtattttttt tcagacactt caggaaatat ttcttttgaa attaatgatg tagattattc
     7861 aaataatagc ggtgaattta ccgtaaatgt atatcatctt aaaaagagcg gcgatttggg
     7921 cagaaattca tataataagt gcccgatgaa agaaccggaa aacggtaaag actgtgaggg
     7981 taatacttgg gtaaaagaag gaataaaagg ttggttttat catggacttt ttaataactc
     8041 atatcgaggc acaggtaaga atagtggttg tcagtgcgtt tatactaaag gtgatttagt
     8101 tgtttcgcat ccggatcaag gtacgtttga ttatggattt tgggaatatc aaggcgatat
     8161 agatgatgtt caagccagac atttgcactt gatatgggat gtattgcctc atgatttatg
     8221 gattggtaaa aaaacataca atgaccctca tataatctgc aaatgaggtc atatatattt
     8281 tagaatcaga attgatctaa tacggcggtt tgtgtcggtt gttatctggt cttctatgga
     8341 ttcattttac gatggg
//
LOCUS       DAOQNQ010000129         8323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3159_length_8323_cov_6.004838, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000129 DAOQNQ010000000
VERSION     DAOQNQ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8323
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3159_length_8323_cov_6.004838"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /gene="holA"
                     /locus_tag="PK754_09050"
     CDS             <1..973
                     /gene="holA"
                     /locus_tag="PK754_09050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNB09692.1"
                     /translation="MYLLYGSEVFLADQLADAVVQAVFNAPKDDFNLHIFYGKEAPLD
                     TVISTALGFPVMADRQVIILREADQWKLTKDKRDDLKDLFPKFPDTTVFIAIGSGIDV
                     RTNPWAWMRKNGIAIEFKPLRENEIPVWIDQFLDERGKKITDQASLLLRTRVDLSLRE
                     VHNQIDKLCTYIGDRDTINEEDVEEAVGISRQYNIFEFCNAIGKRDLTKAMTVYEQMA
                     RFGGEPVYMIVMLTRHYHILLKMCEMRDQGTAPSQIQSVMMTENRIFPNFFQTDYWPQ
                     AQKHKTSDVKLAFRCLLQADIQLKSSAVDKDMLMQHLIYQLATSTAV"
     gene            970..1632
                     /locus_tag="PK754_09055"
     CDS             970..1632
                     /locus_tag="PK754_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB09693.1"
                     /translation="MTSEESKHDLISESLRELVRTPHLVRQALKNYQKLSDEEVINRF
                     QEGDLVAYDVIVARYKDQLINYVMSYVGEKKDAEDIVQDTFVKIYRFKQMYKRIAKFS
                     TWVYTVAGNLAKSELRKRKRQQLYPISSLGTDEKEFEAVETRVNADELTDSSVKKDLI
                     KKAIKTLPERYREVIRLREIENLSYEEIAEQTQLPIGTVRSRINRARVQLQSKLKFLV
                     EK"
     gene            1629..2126
                     /gene="moaC"
                     /locus_tag="PK754_09060"
     CDS             1629..2126
                     /gene="moaC"
                     /locus_tag="PK754_09060"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016861268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNB09694.1"
                     /translation="MKTSKKLSHVDHRGKATMVDVSGKSDTERSATAMARVTMSAAAF
                     DAIQNNAIKKGDVLTVAQLAGIMAAKKTSDLIPLCHPLNLSRVDVACFPEPDSLSIRI
                     EATCKLLGKTGVEMEALTAVTIAALTVYDMAKAMDKKMVIRDICLLEKSGGKSGIFRN
                     NKMKP"
     gene            2132..2908
                     /locus_tag="PK754_09065"
     CDS             2132..2908
                     /locus_tag="PK754_09065"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNB09695.1"
                     /translation="MRFFFCRIVFIGAILWAPLSAQQIPIGEHTSYKLSYLSFSAASV
                     SFQVTGIVNINDRPCYRLVTDAQTTSFFSAFYDLHNTYETFVDTATGLPLRYTKNIAQ
                     KTLRQHLTINYDHDNQTAQYDGGKWDSVFTVNVQPQSHTLFSALFDLRRRTMYEGKQW
                     VYNLDNETEPWKIQVTVLSRENIPAAGKEYPAYKLQVRFIPVGEEKKRKHTDIVTRRL
                     VQSATKLYYWIEADAPHRGVQIEHETSPFSTYTILTGTGK"
     gene            complement(2913..3734)
                     /locus_tag="PK754_09070"
     CDS             complement(2913..3734)
                     /locus_tag="PK754_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09696.1"
                     /translation="MLKHKVEPASDSAAAQDHRKVAWIDRGWYVAIEFEMASSGKLDQ
                     AIAIAKKNPFFVELVDERGMTWFRAIYFKKDFSKFQELYALAGPWKNTRFFLKGDEVK
                     KDDFETWYACYQQYWGHRKNLNLQDYCGTNKVSVYPDFIGCYGRNIVLRWQDPLTYYA
                     QYHTKLWYTFGKRQGNTFHLDKAAMRGFLKKQNAEYELCPCYGHESIDHYINKLPNDL
                     NPHIHTEWQFKEDFLKITANRAFIHYDITLSMMPEICPVNEKSYEKFMNRIFKES"
     gene            complement(3849..5249)
                     /locus_tag="PK754_09075"
     CDS             complement(3849..5249)
                     /locus_tag="PK754_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09697.1"
                     /translation="MRFQKFLRVMLMLVIAGAFSVSAQEEGGNPTTGEGASSSGGFDF
                     QGPYDGSGGSQFTMPAGVTTIDGKSYISLRIQPELTLGKFGMGLDVPLLFSTENGKLR
                     TDEFKGGVGPLRMIRYIRYGVKHKDPVYARLGDLTGTTMGYGLIMYNYTNGISFEKRK
                     IGMNVDINYEQQYGFEAVYSDFDRVSIYGVRPYVKPFKQTDIPIIKSTEFGATYMTDR
                     DNKTAIQGMTVMGVDAGITVVDNGFLQIQPYTEFARMLKNDDLADSAASMGKSYGAGQ
                     GFALGSNFRFNLIANVFQLGVKLERRIFSSDFMPQYFDAVYEINKDAKAYKVVDAEAV
                     QGNYGEIYANLIGKIQFVGGISIPDNAKKSESAYIHIGMSAPNLIPKVIVSGTYDKGY
                     LDGVADAFRFRSKRSVAHLILGYEAYTMGPFVMQAGIDYKWTFIEKKDKSLKAVNYVM
                     PFVGITTKLPFGDQKK"
     gene            5420..5647
                     /locus_tag="PK754_09080"
     CDS             5420..5647
                     /locus_tag="PK754_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015363832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HNB09698.1"
                     /translation="MNISFQKIIYPLLASLTLGLAPFFPEPHIWKQIVNIANGTFTEP
                     LDWFDLAMHGAPWIWLIYALLKQSKKPVEPS"
     gene            6110..>8323
                     /locus_tag="PK754_09085"
     CDS             6110..>8323
                     /locus_tag="PK754_09085"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09699.1"
                     /translation="MKTMLRLVRYTTLLCFSVLAFAPVYGQQSVPSWISQDKSFKETS
                     KEFEQYWKGKDVHDKAVIRGKGFKQFNRYQWFWSTRTNAQGVFDPSLYLEAKAEKYSN
                     FPNSNFAPRRGMNRAMVNSSNWTTLGPQTAIPTNGGAGRVNCIVFDPSNSNTIYIGTP
                     GGGLWKTTNGGTSWTTNTDELGTLGVSHLAIDPTNTNTMYLATGDNDAGDTYSIGILK
                     SVDGGLTWSATGLTWTANQLYRISKISINPTSTSTLVATTSNGIYRSTDAGATWTQVQ
                     TGNFKDLDVNPSNYNEQYASFYGNGTASIYKSANGGSTWTKLTSGLPTTGVLRCAVAI
                     APSTPSTVYAIYCDNTSGGYGLYGIYKSTNSGSTWTQVKTSASPNVLGWNSNGGDTGV
                     GQGWYDLCITVSPTDANTVYTGGVNIWRSTNGGTTWTLNAQWQGSGAPYVHADHHAIE
                     FLPGSGTTLFSGNDGGFFKTTNSGTTWTDFSNGLGIHQYYRISAAKTSANTILGGAQD
                     NGTDRYNGTSWSRIIGGDGMECQVDYTNANIQYGALYYGDLYRTTTGGTPSNISDPTE
                     DGAWVTPYVLHPTTNTTMYYASASKINRSTNITNTNPTWTAISGTLGSASDPLTVLAV
                     SPSNGTTILTANAAGAWKGTASGSTWTFTSLSSSGLPAGVTSFAFHPTDVNTAWATIG
                     GFTAGSKVYKTTNGGTSWTNISGSLVNVPANCVAIHPSTPTDVYIGTDLGIFYSAD"
BASE COUNT         2287 a         2008 c         1812 g         2216 t
ORIGIN      
        1 gatgtatctg ctctacggca gcgaagtttt tctcgctgat caattggccg atgcggtcgt
       61 tcaagctgtt tttaatgccc ccaaagacga ctttaatttg catatttttt acggcaaaga
      121 agcgccgctg gatacggtga tcagcacggc cttgggtttt cctgtcatgg ccgatcggca
      181 agttattatt ttgcgcgaag ccgatcagtg gaaactgact aaagataagc gcgacgacct
      241 caaagatcta ttccccaagt tcccggatac taccgtattt atagcgatcg gcagcggcat
      301 cgatgtgcgt accaatccct gggcatggat gcgaaaaaac ggcatcgcca tcgaattcaa
      361 accgttacgc gaaaatgaaa ttcccgtatg gatcgatcaa tttcttgatg aacgcggaaa
      421 aaaaatcacg gatcaggcgt cgttgttgct tcgaacgcgc gtagatctat cgctcagaga
      481 ggtgcataat cagattgata aattatgcac gtatatcggc gatcgcgata cgatcaatga
      541 agaagatgtc gaagaggccg tcggcatttc ccgccaatac aatatttttg aattttgtaa
      601 tgccatcggt aaacgcgatc tgactaaagc catgacggtg tacgaacaaa tggcgcgttt
      661 tggcggtgaa ccggtctaca tgatcgttat gctcacgcgg cactatcata ttcttctcaa
      721 aatgtgcgaa atgcgtgatc agggcaccgc gccttcacag atccaatccg tgatgatgac
      781 cgaaaaccgg attttcccca atttttttca aaccgactat tggccgcagg cgcagaaaca
      841 taaaacatcg gatgtcaaac tcgctttccg ttgccttttg caggcggata ttcagcttaa
      901 gtccagcgcc gttgacaaag acatgctcat gcaacatctg atctaccagc tcgccacgag
      961 taccgccgta tgacatccga agaatccaaa cacgatctca tcagcgaaag cctccgcgaa
     1021 ctggtgcgta cgccgcattt ggtacgacag gcattaaaaa attatcaaaa attgtcggac
     1081 gaagaagtga tcaaccgttt tcaggaaggc gatctcgtag cttacgacgt gattgtggcc
     1141 cgatacaaag atcagttgat caattatgtg atgagttatg tcggcgaaaa aaaagatgcc
     1201 gaagatatcg tgcaggatac gtttgtcaaa atttaccgtt tcaaacaaat gtataaacgc
     1261 attgccaagt tttctacatg ggtctatacc gtcgccggca atcttgccaa aagcgaactg
     1321 cgcaaacgca aacgccagca actgtatccg atttccagcc tggggacgga tgaaaaagaa
     1381 tttgaagcgg tcgaaacgcg ggtcaatgcg gatgaactca cggattcctc cgtgaaaaaa
     1441 gatttgatca aaaaagcgat taaaacactg ccggagcgat accgcgaagt gattcgcctt
     1501 cgtgagatag aaaacctttc gtacgaagaa attgcggaac aaacgcaact tccgatcggc
     1561 accgtacgtt cgcgcatcaa tcgcgcccgt gtacaactgc aatccaaatt gaaatttttg
     1621 gtggaaaaat gaaaacgtca aaaaaacttt cccacgtgga ccatcgcggc aaagcgacta
     1681 tggtcgatgt ttccggaaaa tcggacaccg aacgtagtgc aacggcaatg gcgcgcgtta
     1741 cgatgagcgc ggcggcattt gatgccatac aaaacaacgc cattaaaaaa ggcgatgtac
     1801 tcacggtagc acaactcgcc ggaatcatgg cggccaaaaa aacttcggat cttattccgt
     1861 tgtgccatcc gcttaacctg agccgtgtgg atgtcgcctg ttttcctgaa ccggactcat
     1921 tgtccattcg aattgaagcg acgtgcaaac tcctgggtaa aacaggtgtt gaaatggagg
     1981 ccctaacggc tgtaacgatt gcggcgttga ccgtttacga tatggccaaa gcgatggata
     2041 aaaagatggt catccgcgat atatgcctgc tcgaaaaaag cggcggcaaa agcgggattt
     2101 tccgaaacaa caaaatgaaa ccttaattct gatgcgtttt tttttctgtc gtattgtttt
     2161 tatcggggcg attttatggg cgccgctttc ggcccagcaa atacccatcg gggaacatac
     2221 atcctataaa ctttcatacc tttcgttttc ggccgcttcg gtttcgtttc aggtaaccgg
     2281 catcgtcaat atcaacgacc gcccatgtta tcgcctcgtt acggatgcac aaaccacatc
     2341 ttttttttcg gcgttttatg atttgcataa tacgtatgaa acctttgtag ataccgcaac
     2401 cggactgcct ttgcgttata ccaaaaatat cgcgcagaaa accctccgcc agcatttgac
     2461 tataaactac gatcatgata accaaacggc gcagtatgac ggcggaaaat gggattccgt
     2521 gtttaccgta aatgtgcaac cgcaatcgca tactttattt tcagcgctat ttgatttgcg
     2581 acgacggacg atgtacgaag gaaaacaatg ggtttacaat ttggataatg agaccgaacc
     2641 gtggaagata caggtcaccg ttttatcgcg tgaaaatatc ccggcggcag gaaaagagta
     2701 tcccgcttac aaattacaag ttcgattcat ccccgtcggc gaagaaaaaa aacgaaagca
     2761 taccgacatc gttacgcggc gattggtaca aagtgcgaca aaactatatt attggattga
     2821 ggcggatgcg ccgcatagag gtgtgcagat cgaacatgaa acgtccccgt ttagcaccta
     2881 cactattctg accggtaccg gtaaatagat cattacgatt ctttgaatat cctattcatg
     2941 aatttttcat aggatttttc attgaccggg caaatctcag gcatcatgct caacgtaatg
     3001 tcataatgaa taaacgcacg attggccgta atcttcagga aatcttcctt aaactgccat
     3061 tcggtatgaa tatgcggatt aaggtcgttg ggaagcttat tgatatagtg gtcaatggat
     3121 tcatgaccgt agcaagggca tagttcgtat tcggcatttt gttttttcaa aaatccacgc
     3181 atcgccgctt tatccaaatg aaacgtatta ccctgccttt ttccgaatgt gtaccaaagt
     3241 ttggtatgat actgcgcata gtatgtcagc gggtcttgcc accgtagtac gatatttctc
     3301 ccgtagcagc cgataaaatc aggataaacg ctgactttgt ttgtaccgca gtagtcctgc
     3361 aggtttaggt tcttccgatg accccaatat tgctgataac aggcatacca cgtttcaaaa
     3421 tcgtcttttt tgacttcatc gcctttgaga aaaaaacgcg tgtttttcca cggccctgcc
     3481 agcgcataca attcctgaaa cttggagaaa tcttttttga aataaattgc acgaaaccac
     3541 gtcatgccgc gctcatctac cagctctacg aaaaatggat tttttttggc tatggcaatc
     3601 gcctgatcga gcttaccgga cgaagccatc tcaaattcga tagccacata ccaaccgcgg
     3661 tctatccagg ctactttgcg atgatcttga gcggcggcag agtccgaagc cggttcgact
     3721 ttatgtttaa gcatattcct gtttaatatg attccgtatg acttatactt tgagtataat
     3781 aaaaaacctt caacaaaaaa aggctgcctt tcgacaggca gcctttgaaa tccatttatt
     3841 ctttcaaact attttttctg atcgccgaaa ggcaatttgg tcgtaatgcc cacaaaaggc
     3901 atcacataat taacagcctt cagagatttg tcttttttct cgatgaaagt ccatttgtag
     3961 tcaataccgg cttgcatgac aaaaggtccc atcgtatagg cttcataacc gaggatcaaa
     4021 tgggctacgc tgcgtttgct gcgaaaacga aatgcatccg caacaccgtc taagtatcct
     4081 ttatcatacg ttcccgatac gataacttta ggaatcagat taggtgcgga cattccgata
     4141 tggatataag cgctctcgga ctttttagca ttgtcgggaa tactgatacc gcctacaaat
     4201 tgaatcttgc cgatcagatt ggcatagatt tcaccgtagt tgccctggac ggcttcggcg
     4261 tctacgactt tgtaagcctt agcgtcttta ttgatttcat agaccgcgtc aaagtactgc
     4321 ggcataaagt cactgctgaa aatacgacgt tctaatttta cgcctaactg gaatacattg
     4381 gcaatgagat taaatcggaa attacttccc aacgcaaaac cttgccccgc accgtaactc
     4441 ttgcccatgc tcgcggcgga gtcggcgaga tcatcgtttt tcaacatgcg tgcaaattct
     4501 gtataaggtt gtatttgtag aaaaccatta tctacaaccg tgataccggc atctacaccc
     4561 atgacggtca taccttgaat ggccgtctta ttatcacgat ccgtcatata cgttgcgccg
     4621 aattccgtgc ttttgatgat cggtatatcc gtttgtttga aaggtttgac gtacggacgc
     4681 acaccgtaaa tgctcacgcg atcaaaatca ctatacaccg cttcgaaacc gtattgctgt
     4741 tcgtaattga tatctacatt cataccgatc ttacgttttt caaagctgat tccgttggta
     4801 taattataca tgatgagacc ataacccatc gtcgttccgg tcagatcacc caaccgcgca
     4861 tagacaggat ctttgtgttt tacgccataa cgtatatagc gtatcatacg caacggcccg
     4921 acgccgcctt taaattcatc cgtacgcagt ttgccgtttt ccgtgctaaa aagcagaggc
     4981 acgtccaaac ccataccgaa ttttccgagc gtcagttcgg gttgaatacg caaactgata
     5041 taactttttc cgtcaatcgt tgtcacaccc gccggcatgg taaattgact tccgccggaa
     5101 ccgtcataag gtccttgaaa atcaaagccg cccgacgaag aagcgccttc accggttgta
     5161 ggattgccgc cttcttcttg tgctgacacg gaaaacgctc ccgcgataac caacatcagc
     5221 atgacacgta agaacttttg aaaacgcatt gtacctcctg acattattaa atgtacgcca
     5281 ttcatccgat taaacggggt aaggtaaaac gaatatcgtc tatttgcaaa cttgaaaata
     5341 tatgccgaca aaataaaatc tttgagcata caggctaaga tcatatattg actccataat
     5401 tctattatcg gattgacgta tgaatatttc atttcaaaaa attatttatc ctttactcgc
     5461 cagtctgaca ctcggtctag cacccttctt tccggaacct catatttgga aacaaatcgt
     5521 taacatcgcc aacggaactt ttaccgagcc gctggattgg tttgatttgg ccatgcatgg
     5581 cgcaccgtgg atatggctga tctatgcgct cttaaaacaa tccaagaagc ccgttgagcc
     5641 gtcatagaat ttttaaatct gatataattt ctttgtaata tatcacacgg attctacaaa
     5701 cccttgcctg tagcggttct acagtgattt ttttattttt tttttttact tcttttttta
     5761 gacagcctat acttgagccg ttcttatctt ttcaagttat tcctttctga tttttttatt
     5821 cacttcggtc gcctgtgacc aatccgcaaa agcatttcct ttgctttggc gttacgattt
     5881 aaaattcttc tgctgcgtaa attaattaac gcagattgtc ggtattgaca aaccttactc
     5941 ttatgaggag gagtatgagg cttgttgttc atcaccgcat tccggcttta caaaggccct
     6001 cacttttttg cccaccctga acggtacgga taccgtttcg tttttcatta cttcattttt
     6061 attaaataac aaattttcca acggcatggt tatgaggaga gaataaccca tgaagacaat
     6121 gttacgccta gtgcgttata ctacactatt atgttttagt gttctggcct ttgcgccggt
     6181 gtatggacaa caatccgtac catcctggat ttcacaggac aaatcgttta aagaaacatc
     6241 gaaagaattc gaacagtact ggaaaggaaa agatgttcat gacaaagccg tgattcgcgg
     6301 caaaggattc aaacagttta atcgttatca atggttttgg agtacgcgta ccaacgcgca
     6361 aggtgttttt gatcccagtt tgtaccttga agccaaagcc gaaaaatatt ccaattttcc
     6421 aaattccaat tttgcaccgc gccgcggtat gaatcgcgcg atggtcaaca gttccaactg
     6481 gaccacgctc ggtccgcaaa ccgccatacc gacaaacggc ggcgcaggac gcgtgaattg
     6541 catcgttttt gatccgagca acagcaacac gatttatatc ggtacgccgg gcggcggtct
     6601 ctggaaaacc accaatggcg gtacatcgtg gacaaccaat acggatgaat tgggtacttt
     6661 aggcgtgtcg catttagcga ttgaccctac caataccaat acgatgtatc ttgcaaccgg
     6721 tgacaacgac gccggcgata cgtattccat cggtattctc aaatccgtgg acggcggctt
     6781 gacctggtcg gcgacaggat tgacatggac agccaatcaa ctttatcgta tttcaaaaat
     6841 cagcatcaat cctacgtcca caagcacgct cgtcgctacg accagcaacg gtatttatcg
     6901 ttcgacggat gcgggtgcca cttggacaca agtacaaacc ggtaacttca aagatctgga
     6961 tgtcaatcca tccaactata atgaacagta cgccagtttt tacggcaacg gcaccgcttc
     7021 tatttataaa tccgccaacg gcggctcgac atggactaaa ctgacttccg gccttccgac
     7081 gacaggtgta ttgcgttgcg cggtagccat cgcgccttcg acaccgtcca ccgtgtatgc
     7141 gatttattgt gataacacct cgggcggtta tggtctctat ggtatttata aatctaccaa
     7201 tagcggttcg acgtggacac aagtcaaaac ctccgccagc cccaatgtac tcggatggaa
     7261 tagtaacggc ggtgataccg gtgtcggtca aggctggtat gatctctgca tcaccgtttc
     7321 cccgacagat gccaatacgg tttataccgg cggtgtaaac atctggcgtt ctaccaatgg
     7381 tggtacaaca tggacactga atgctcagtg gcaaggatca ggcgctcctt acgtacatgc
     7441 cgatcatcat gcgatcgaat ttttacccgg cagcggtacc actttgtttt ccggtaacga
     7501 tggcggattc ttcaaaacta ccaatagcgg aacaacatgg accgatttta gtaacggcct
     7561 cggaatccat cagtattatc gtatcagtgc agccaaaacc agcgctaata cgatactcgg
     7621 tggcgcacag gataacggca cggatcgtta taacggaacg agctggtcac gcatcattgg
     7681 cggcgacggt atggaatgtc aggtagatta taccaacgcc aatattcaat acggtgcatt
     7741 gtactacggc gatctgtatc gcacgactac aggcggtacc ccgtccaata tttccgatcc
     7801 tacggaggat ggcgcttggg tgactccgta cgtgttgcat ccgacgacga atacgacgat
     7861 gtactacgct tccgccagca aaattaaccg ctcaactaat atcaccaata cgaacccgac
     7921 gtggacagct atcagcggta ccctcggttc cgcaagcgat cctttgacag tattagccgt
     7981 atcgccttcc aacggaacga cgatcctcac agctaatgcg gcaggcgctt ggaaaggaac
     8041 agccagtggc tcgacctgga catttacaag cttgtcgagc agcggtttgc cggccggtgt
     8101 tacttcattt gcatttcacc ctacggatgt aaacaccgct tgggcaacca tcggcggttt
     8161 cacggcaggt agcaaagttt acaaaacaac aaacggcggt acttcgtgga caaatatttc
     8221 cggttccttg gtcaatgtac ccgctaactg cgttgctata catccctcga caccgaccga
     8281 tgtgtatatc ggaacagatc ttggtatatt ctactctgcg gac
//
LOCUS       DAOQNQ010000130         8217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3226_length_8217_cov_6.069223, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000130 DAOQNQ010000000
VERSION     DAOQNQ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8217
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3226_length_8217_cov_6.069223"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PK754_09090"
     CDS             complement(<1..388)
                     /locus_tag="PK754_09090"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNB09700.1"
                     /translation="MKIKDISLTISNDIVTWPGDPKVNIQLPIQMKKGDACNVSRWEI
                     GAHTGTHTDAPFHFVDDGKGIDDVPLDIYIGPCIVVEVKPKTINIEADDLKHIDFKGH
                     KRVLFKTKNSLHWKNGNMEFDKDFIAV"
     gene            complement(462..1667)
                     /locus_tag="PK754_09095"
     CDS             complement(462..1667)
                     /locus_tag="PK754_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB09701.1"
                     /translation="MNLDFQNKYDELINSLPDGIFVINENGQIILANQSAAEILGYYG
                     PSEFYGLTVDTIYANTGDHDALLSILGHRGYAERSLFDWRKKDGSTRLIEVSAIPLRN
                     AQGKIGGIQAVFRDISRRLENQIAQQETIAEAASRAVDADRILEYINFYRTRPMSLIL
                     QGVAHNLNTPLGSMRGRAELLLHQLRKNASLFESVSDEKLRSDLDGFRVKLEKGLNEV
                     IGQVDKASLLMRGFSAKVAFEMQDAEMELDINAILQNEFGFFESSLYFKHQIQKEMQY
                     ADKLPMIKGIYRDFSQAFHNIIIHCIKATAAISDRWLYVQTELIDNMITVTIRDNRVM
                     AAHDSEAYHGRMNLETRYSALSDENFFSMGDIELFNARQYLAKYQATMTLFPNGEPKI
                     IVQIPRNWN"
     gene            complement(1674..2582)
                     /gene="prmC"
                     /locus_tag="PK754_09100"
     CDS             complement(1674..2582)
                     /gene="prmC"
                     /locus_tag="PK754_09100"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNB09702.1"
                     /translation="MSSAVPEIWTVKSIMDWTIHYLSEKNIESARTNVEWLLCHTLGC
                     KRMDLYVNYDRPLDQNERDEFKASLKRRLLAEPLQYIVGHTDFMGLTFHVNRNVLIPR
                     PDTELLVERVLDLCGKSENIKSLRILDIGTGSGAIIISLAYYLKKRGFELQATAVDIS
                     QNALVVAQENANKLLPGFHIQWQHGDIFDPSSIESLKHRFDIIVSNPPYISDKEYALL
                     DKEIRDYEPVTALRAEDNGLAFYRRIGHIAADILDPSSAFGYIALEVGFDQAEAVAQI
                     LHSAGYTYQQCYKDIQNYERVLIGKK"
     gene            complement(2586..3119)
                     /locus_tag="PK754_09105"
     CDS             complement(2586..3119)
                     /locus_tag="PK754_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09703.1"
                     /translation="MQKILCWVGIIPFTISAAIFIPALMSDLITGNYTTTEQSKLSAL
                     QNDSLVALSDNPHSQKPTELGALIGAGICMGIVPGILTFLLIVQLRKINRLEKKHNEV
                     YIQDRLLELAQKHNGKLTIVETATELDIAVSDAQTLLENLCRIGMAQLQVSESGTLVY
                     HVTDIISKDEKDRSETV"
     gene            complement(3152..3940)
                     /gene="thiD"
                     /locus_tag="PK754_09110"
     CDS             complement(3152..3940)
                     /gene="thiD"
                     /locus_tag="PK754_09110"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNB09704.1"
                     /translation="MNPRKALTIAGSDSGGGAGIQADLKTFSALGVYGASVITAITAQ
                     NTVGVTAIMDVTTDVISRQIEAVLTDIKPQAVKTGMLSTADIIQTVSDSLSKYGVKKL
                     VVDPVMVAKSGDRLLQDNAISALIERLLPQATVLTPNIPEAEVLTGKKITTISDMEIA
                     ADTLRAMGPKYIIVKGGHLKGEAIDVFYDGRKIYHLRHQRIRSKNTHGTGCTFSAAIA
                     AHLAKGFTVLESVRLAKRYISMAITHAFSVGSGHGPVHHFHKFY"
     gene            complement(3956..5758)
                     /locus_tag="PK754_09115"
     CDS             complement(3956..5758)
                     /locus_tag="PK754_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNB09705.1"
                     /translation="MSAQEITTTMKYSDHPIVQKLLEEIVSHLTDLSADRTDRIIGLV
                     RSFTENQLVHEKKLTRIGSALSSERNLDALLEMIVVEAMSFTNADGGTLYIKTPDDRG
                     IAFKIMRTKSLNFAMGGTSGKTIPFPPVKLIKDDGTRNEQQVSAYVALTGNTVNIPDV
                     YDVEGFDFQGTKAFDKNTGYRSKSMMVIAMRNHENEIIGVVQLLNAKAHDGTTIPFSV
                     DYQELIESLASQAAVAITNTTLINDLRALLDSFIQVIAAAIDEKSPYTGGHIRRVAEL
                     TSAMARSMSDTSEGKYKDFTMNEDEHNELRIAGWMHDIGKITTPEYVVDKSTKLETIY
                     DRIHTVMAKFEVLKREEEVRYIRERAKIETSKARDKKKKLSSLEESYAQKKKQIEDDA
                     AFIKVCNIGGEFMEDAKLERLRNIAQQKIIMEGQEMQLLTENEVYNLSIRRGTLTEEE
                     RKKIQDHVVLTIKMLQQLPWPNKLSRVTEWAGAHHEALNGKGYPYGLTAEQLNTQQRI
                     MAVADVFEALTAADRPYKPGKKISECIKILGFMVKDGHLDPDIVDYFITTGMAQDYAR
                     RELKDYQLDVFTYNGKEYDCRRPAQEPTPETATS"
     gene            complement(5807..6364)
                     /locus_tag="PK754_09120"
     CDS             complement(5807..6364)
                     /locus_tag="PK754_09120"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HNB09706.1"
                     /translation="MNSKSKAIAVDAGMLKDFLLVIGFSILTAVSAWVEVPLFFTPVP
                     ITLQTLMVSLSGALLGARRGALSQAAYLFYGICGLPVFAGGASTIAYLIGPTGGYLLA
                     FPIMAFLTGMLVMKESNLLYNIGAFFLASLPVLLIGTLQLKLYTGQGMATAFSLGFLP
                     FIAGDFIKCTLAAVIHKSWNAYRSR"
     gene            complement(6480..8108)
                     /gene="rny"
                     /locus_tag="PK754_09125"
     CDS             complement(6480..8108)
                     /gene="rny"
                     /locus_tag="PK754_09125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNB09707.1"
                     /translation="MDSLSVIIMCAAGIVMFILGWVFATYFGKNKVSEIRKIAENTIR
                     EAKREAETIRNEAEVKSKQRWHQIKMKIVSETDEREKRLKQYEQKLVNREREIRNKMS
                     GTVEKEQLLQRSQKDLTTQQEQLQTRAQHVESVIHQQMEKLENITKLSYEEAKKQFLE
                     SVAQRAQKEAAELMLQMKNEATQKASFEAKWIISDAIQRLASDQTAEITLTTVDLPNE
                     KIKGAIIGREGRNIKAFEQITGVKVIIDDTPEVLVLSSMDPVKREIARLAMLRLTETP
                     NIQPEFIEDVVERCKKQVETAMNKASDEVLNSLNIKNVAPEMKQMLGRLKYRTSYGQN
                     VLQHSKEVALLAGNMASELGYDVQLARRAGLFHDIGKATSNENEGSHVTIGVEVTTRC
                     KEHEVVINSVLAHHDEAEPIHPISQLVTAADIISGSRPGARREPLEAYGARIEKLEQI
                     AQQFDGVSKVYAIFAGREIRIVVEAEKMDDAQSAMLSSNVAQKIQNEMQYPGQIKVVV
                     IRERRVVRYTNSTSPAVDEARIEEAVVSDSPSQN"
BASE COUNT         2112 a         1819 c         1863 g         2423 t
ORIGIN      
        1 ctacggcaat aaaatcttta tcaaactcca tattaccgtt tttccagtgc aaggagtttt
       61 tggttttgaa aagaacacgt ttatggcctt tgaaatcaat gtgtttcaaa tcgtcagctt
      121 cgatattaat cgttttaggt tttacttcta cgacaatgca gggcccgata taaatatcca
      181 aaggcacatc atcaatacct ttgccgtcgt caacaaaatg aaagggtgca tccgtgtgcg
      241 tgccggtatg agcgcctatt tcccagcgtg atacattgca cgcgtcgcct tttttcattt
      301 gtatcggaag ttgaatatta actttaggat cgcccggcca ggttacgatg tcattggaaa
      361 tcgtgagaga aatgtctttt attttcatgc gtcacctctc caaaaaaatt atgattaata
      421 aacacgaatt acgaaaggcg tcgaaaaaga agggcgcgtg attaattcca atttcgtgga
      481 atttgcacga taatttttgg ctctccgtta ggaaagagtg tcatcgtagc ctggtatttg
      541 gccaaatatt ggcgagcatt aaaaagttcg atatccccca tcgaaaagaa attttcatct
      601 gaaagggccg agtagcgcgt ctcaagattc atacgtccat gatacgcttc cgaatcgtgc
      661 gcggccatta cgcggttatc acgaatcgta acggtaatca tattgtcaat caattccgtt
      721 tgcacataaa gccaacgatc agaaatggcg gctgttgctt tgatgcaatg gatgatgata
      781 ttgtgaaatg cttgtgaaaa atcgcgataa atacctttga tcatcggtaa tttatcagcg
      841 tactgcattt ctttttggat ttgatgtttg aaatatagac tcgactcaaa aaatccgaac
      901 tcattttgca aaatagcgtt aatatccaat tccatttcgg catcttgcat ttcaaaagca
      961 actttagcag aaaaacctcg catcaaaagg ctggctttat ctacctgacc gataacttcg
     1021 ttgagtcctt tttccaattt tacgcgaaat ccatccaaat cgctgcgcag tttttcatcg
     1081 ctgaccgatt cgaaaagcga tgcatttttt cgcagttgat ggagaagtaa ttcggcgcgt
     1141 ccgcgcatac taccgagcgg ggtattcaga ttatgagcca ccccttggag tataagcgac
     1201 atcgggcggg tccggtaaaa attgatatac tccaaaatcc ggtccgcatc tacagcgcgc
     1261 gaagctgctt cagcgatggt ttcctgttgt gcgatttgat tttcaagacg ccggctgatg
     1321 tcgcgaaaga cagcttgaat gccgccgatc ttaccttgtg cgttgcgaag cggtatggct
     1381 gaaacttcaa tgaggcgcgt cgaaccgtct tttttgcgcc aatcgaaaag gcttcgctcg
     1441 gcataaccgc gatgccctaa aatagatagt aacgcatcat ggtcgccggt attagcatat
     1501 atcgtatcaa cggtgaggcc gtaaaattca ctcggaccgt agtatcccaa tatttctgcg
     1561 gccgactgat tggcaagaat gatttgacca ttttcgttga ttacaaaaat tccgtccggt
     1621 aggctattga taagttcgtc gtatttattt tgaaaatcta aattcatgga gatttatttt
     1681 tttccgatca gaacacgttc ataattttga atatctttgt aacattgctg atatgtatat
     1741 ccggcactat gcaaaatctg agctacagct tccgcctgat caaagcctac ttccagcgca
     1801 atatatccaa atgccgagga gggatccaaa atatccgcag caatatggcc aatcctacga
     1861 taaaaagcca gcccattatc ttcggcgcgc aaagcggtaa ccggttcata atcacgtatt
     1921 tctttatcca aaagagcgta ctccttatca gaaatgtacg gcggattgga tacgatgata
     1981 tcaaaacgat gtttcaaact ttcgatcgaa gatggatcga aaatgtcacc atgttgccac
     2041 tgaatatgaa agcccggtaa aagtttattc gcgttttctt gtgctacgac caatgcgttt
     2101 tgacttatgt caacagctgt agcttgtaat tcaaaaccgc gttttttcaa ataatatgca
     2161 agactgatga tgattgctcc acttccggtt ccgatatcca gaatgcgaag tgattttata
     2221 ttttcacttt ttccgcataa atccaggacg cgttcaacta aaagttcggt atcgggtctt
     2281 gggattaaca catttcgatt gacatgaaat gtcaatccca taaaatccgt atgtccgacg
     2341 atatactgaa gcggttcggc aagcaatcgt cgttttagcg aagccttaaa ttcatcgcgt
     2401 tcattttgat ccaacgggcg atcataattg acatacaaat ccatacgttt acaacccagc
     2461 gtatggcata aaagccactc tacgttggta cgtgcggatt cgatgttttt ttcactaaga
     2521 taatgtatag tccaatccat gatggatttg actgtccata tctcaggcac ggcagaactc
     2581 atgatttata cggtttccga tcggtctttt tcgtctttag agataatatc tgtgacgtgg
     2641 tacaccaagg ttccggattc gcttacttgt aattgggcca tgccgatgcg gcataaattt
     2701 tccaataaag tttgggcatc cgaaacagcg atatccagtt ccgtagcggt ttccacgata
     2761 gtcagtttac cgttatgttt ttgtgccagc tcaagcagac ggtcttggat atacacttcg
     2821 ttgtgttttt tctcgaggcg atttattttg cgcagttgta cgatcagcag aaaagtaagt
     2881 atgcctggaa cgatacccat acaaatgccc gctccgatga gtgcgcccag ttccgttggt
     2941 ttttgggaat gcggattatc agacagtgcg actaaagaat cgttttggag tgcggataat
     3001 ttagattgtt cagttgtagt atagtttccc gtaatcagat cggacatcag tgcgggtata
     3061 aaaatagccg cgcttatcgt gaaggggatg ataccaaccc aacacaatat cttttgcata
     3121 agaaaggagc gatgggcgac gggcatagag attaatagaa tttatgaaaa tgatgtacag
     3181 gcccatgtcc gctgcctaca gaaaaagcgt gggttatggc cattgaaata tatcgtttcg
     3241 ctaaacgtac agattccaga accgtgaagc ctttagccaa atgtgcggcg attgctgcgc
     3301 tgaaggtgca cccggtaccg tgagtatttt tgcttcgaat acgttggtgt cgcaaatggt
     3361 aaattttgcg tccatcgtaa aaaacatcaa tagcttcacc tttgaggtga ccgcccttta
     3421 ctatgatgta cttaggaccc atcgcgcgca gcgtgtctgc ggctatctcc atatctgaaa
     3481 ttgttgttat tttctttccg gtgagcactt ccgcttcggg aatattaggc gttaggaccg
     3541 tagcctgcgg aagaagtcgt tcgataagtg cacttatggc gttgtcttgc aataaacgat
     3601 caccgctttt agcgaccatt acaggatcta cgactaattt ttttacgcca tacttactaa
     3661 gactatcgct gacggtttga attatatcgg cggtggacaa cattccggtt ttgacggctt
     3721 ggggttttat gtcggtgagc acggcttcga tttgtcggga aatcacatct gtggtaacat
     3781 ccatgatcgc ggttacaccc acggtatttt gagccgtgat cgctgtgatg accgatgcgc
     3841 cgtaaacacc gagcgccgaa aatgttttga gatcggcctg aatacctgca ccgccgcctg
     3901 agtcagaacc ggcgatagtc aatgctttac ggggattcat ctgtggtgag gcgtgttaag
     3961 aagttgcggt ttccggagtc ggctcctgag ccggtctgcg gcaatcgtat tctttaccgt
     4021 tgtatgtaaa tacatcaagt tggtaatctt tgagttcacg tcgcgcataa tcctgtgcca
     4081 taccggtcgt aataaagtaa tcaacaatat ccggatccaa gtggccgtcc ttgaccatga
     4141 agccgagaat tttgatacat tcagaaattt tctttccggg tttatacggg cgatcggcgg
     4201 ctgttaaggc ttcaaatacg tcggcgacgg ccataatacg ttgttgtgta ttgagttgtt
     4261 ctgccgtaag tccgtacgga taacctttac cattaagcgc ttcgtggtgc gcgccggccc
     4321 attcggtaac acggctaagc ttattgggcc atggcaattg ctgaagcatt ttgatcgtga
     4381 gaacgacgtg atcctggatt tttttacgtt cttcttccgt cagcgtaccg cggcggatgg
     4441 agagattata cacttcattt tcggtgagca gttgcatttc ctgcccttcc attatgattt
     4501 tctgctgagc gatattacgc agacgctcca gtttggcgtc ctccataaat tcaccgccga
     4561 tattacatac tttgataaat gcggcgtcgt cttctatttg ttttttcttt tgtgcgtagc
     4621 tttcttctaa ggagcttaat tttttctttt tatcgcgcgc tttggaagtt tctatttttg
     4681 ctcgctcacg aatataacga acttcttcct cgcgtttaag cacttcaaac ttagccatca
     4741 ccgtgtgaat acgatcgtaa atagtttcta gttttgtgga tttatcaacg acatattccg
     4801 gcgtggtaat tttgccgata tcgtgcatcc agcctgcgat acggagctcg ttgtgttcat
     4861 cttcgttcat cgtgaaatct ttgtacttcc cttcggaagt atcgctcata gaacgcgcca
     4921 tagcggaagt aagttccgct acacgacgga tgtggccgcc ggtataaggt gatttttcgt
     4981 cgattgcggc cgcgataact tggataaacg aatctaaaag ggcacggagg tcgttgataa
     5041 gcgtggtatt ggtgatggct accgccgctt gcgaagcaag cgattcgatc agttcctgat
     5101 aatccacaga gaaaggaatc gtcgtaccat cgtgcgcttt ggcattgagc aactgaacga
     5161 cgccgatgat ctcattttcg tgattgcgca ttgcaatcac catcatggat ttggagcgat
     5221 acccggtgtt cttatcaaac gcttttgttc cttgaaaatc gaaaccttcg acatcgtaca
     5281 catccggtat attgacggta ttacctgtca gcgcaacata agccgaaact tgttgttcat
     5341 tgcgtgtgcc atcgtctttg atcagtttta ccggaggaaa aggaatggtt tttccgctcg
     5401 taccacccat ggcgaagttg agagacttgg tacgcatgat cttaaaagcg ataccgcggt
     5461 catcgggagt ttttatatac aaggtgccgc catccgcgtt ggtaaagctc atcgcttcaa
     5521 cgacgatcat ttccaaaagt gcgtcgaggt ttcgttccga tgatagcgcc gatccgatgc
     5581 gggtaagttt cttctcatgt accaattgat tttcggtaaa actacgaacc aatccaataa
     5641 tacgatcagt ccggtcagcc gacaaatccg tcaaatgcga cacgatttct tccaataatt
     5701 tttggacaat cgggtggtct gagtatttca tagtggttgt gatttcctgc gcgctcataa
     5761 tctctctcga tacggtaaaa ccgtggtctt taatgatcaa caaagatcaa cgggaacggt
     5821 acgcattcca tgatttgtga atcacggcgg ccaaagtaca tttgataaaa tcaccggcaa
     5881 taaaaggtaa aaatcctaac gaaaaagccg tggccatgcc ctgaccggta taaagtttga
     5941 gttgtaaagt gccgatcaaa agaacaggta aactggcgag gaaaaaggcg ccgatattgt
     6001 aaagcaaatt ggattctttc ataacaagca taccggtaag aaatgccatg atcggaaaag
     6061 ccagcaagta accgccggtg ggtccgataa gatatgcgat cgtggatgcg ccgccggcaa
     6121 aaacgggcaa accgcagata ccgtagaata aataagccgc ctgactcaaa gcgccacgac
     6181 gagcacctaa caaagcgccg gacaaagata ccataagcgt ttgcagtgtg atcggtaccg
     6241 gtgtaaaaaa taacggtact tcaacccacg ctgaaaccgc tgtaagtatg gaaaaaccga
     6301 tgaccaaaag aaaatctttg agcattcctg cgtcaacggc tatggctttg gatttggagt
     6361 tcatatacgt gatcaatcct tacattaaaa atatgttaac agatagggtt tgataacctc
     6421 ttatgtgaga acggtcaaat gtacatccgt gattgaccta ccgcaagtta tttttaatct
     6481 taattttgcg aaggtgaatc actgacaaca gcctcctcaa tacgcgcttc atcaacggca
     6541 ggggaggtac tattggtata tcgtacgacg cggcgctcgc gaatcacaac aaccttaatc
     6601 tgaccggggt attgcatttc gttctgaatt ttttgtgcga cattggagct cagcatggcc
     6661 gactgggcat catccatttt ttcggcttcg acgacgatgc gaatttcgcg tccggcgaaa
     6721 atagcatata ctttagaaac gccgtcaaat tgttgagcga tttgttccaa tttttcgatc
     6781 cgtgcgccat acgcttccag cggttcacgg cgcgcgccgg ggcgagaacc ggaaataatg
     6841 tcggctgccg taacaagttg tgaaatcggg tggatcggtt cggcttcgtc atgatgtgcg
     6901 agtaccgagt taatgacgac ctcgtgctct ttgcaacgcg tcgtaacttc gacaccgatc
     6961 gtgacgtgac ttccttcatt ttcgttgctg gtggctttac cgatatcatg aaacaaacct
     7021 gcgcggcgcg caagctgaac atcatagccg agttcagatg ccatattacc ggctagcagg
     7081 gcgacttctt tgctgtgttg taaaacattt tgaccatagc tggtgcggta cttcagacgg
     7141 cccagcattt gtttcatttc cggtgcgaca tttttaatgt ttagcgaatt gagcacttca
     7201 tcggaagctt tattcattgc tgtttcgact tgttttttgc aacgctcgac aacgtcttca
     7261 ataaactcgg gctggatatt gggcgtctcc gtgaggcgca acattgctaa tctggcgatt
     7321 tcgcgtttca caggatccat cgaagaaaga acaagcactt ctggggtatc gtcaataatg
     7381 accttcacgc ccgtgatttg ttcaaacgct ttgatatttc gcccttcacg cccgatgatc
     7441 gcgcctttga tcttttcatt gggtaaatcc accgttgtga gagttatctc ggccgtttga
     7501 tcggaagcta aacgctggat ggcgtccgat ataatccatt tggcctcgaa acttgccttc
     7561 tgcgtagctt cgtttttcat ctggagcatc agttcggcgg cttctttttg agcccgttgc
     7621 gcaacacttt ccaaaaactg ttttttcgct tcttcgtagg acaacttggt aatattctct
     7681 aacttttcca tctgctgatg gatgaccgat tcgacatgtt gggcacgcgt ctggagttgc
     7741 tcttgttgcg tcgtaagatc tttttgcgaa cgttggagca gttgttcttt ttcgacagta
     7801 ccgctcattt tgttgcggat ttcgcgttcg cggttaacca gtttttgctc gtactgttta
     7861 agacgttttt cacgttcgtc cgtttcactg acgattttca ttttgatctg atgccaacgt
     7921 tgtttggatt tgacttcagc ttcgttgcgt atggtttcgg cttcacgttt ggcttcgcgt
     7981 atggtgtttt cggcgatttt gcgaatttcc gatactttgt tttttccgaa ataagttgca
     8041 aagacccatc cgagaataaa catcacaatg cccgcggcgc acatgatgat aacagataaa
     8101 ctatccataa ctatcctgta agtttcttaa gttttaacta tgttttgaac gaagtctata
     8161 atcattagta tagacgtaaa aaaccgcgca caagcggttt tcactacaaa gaagaat
//
LOCUS       DAOQNQ010000131         8212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3232_length_8212_cov_6.712394, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000131 DAOQNQ010000000
VERSION     DAOQNQ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8212
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3232_length_8212_cov_6.712394"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="PK754_09130"
     CDS             <1..590
                     /locus_tag="PK754_09130"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNB09708.1"
                     /translation="LFSLDNPNKKNYLRAPKFDKCFIKLKLGVRLVAKIDKILSLGDG
                     EHRFHFDVKAEDIGLNSIDFEGRNHFEKPISADAVLTKTNHVYYIKMKVVGEARLVCD
                     RCLEDVSVDVADDFQVIYTDHRSERYAQSGETEIRLLDVRKENQIVLDKDIVDTIELA
                     MPHKVLCDEDCKGLCVQCGANLNERACEHAQTVNE"
     gene            619..801
                     /gene="rpmF"
                     /locus_tag="PK754_09135"
     CDS             619..801
                     /gene="rpmF"
                     /locus_tag="PK754_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006905007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNB09709.1"
                     /translation="MPNPKRRHSKQRRDKRRTHWKLDNAAVGKCANCGQPKMHHRVCP
                     NCGHYDGRQIVIAREA"
     gene            961..1977
                     /gene="plsX"
                     /locus_tag="PK754_09140"
     CDS             961..1977
                     /gene="plsX"
                     /locus_tag="PK754_09140"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNB09710.1"
                     /translation="MKIVVDAMGGDEAPKHIIDGVILAARAWVSTGVNHEIILTGDKD
                     VVMKEIERCGGRDLCGNILRLEHTACVIDMHESLADAWRNKSGSSIHRGAELVKSGQA
                     DAFIGMGNTGAMMTAGLLGVGRIEGVQRPTIGAFFPTARGRSLVLDVGAVTDCKPQHL
                     LQFGLMGSIYMEAMWGVKNPTVGLLSIGEEDSKGNEVTKETNALFREKKLFNFAGNVE
                     GRDILKGTTDVIVCDGFVGNIVLKFGESVPGFLKARFKQAAESSFMTKLQLLLAKTGI
                     KGVFKDMNYEEYGGVPLLGVNGVVIIGHGSSSPKAMYNAIMVAQKMVDKKINEVIKER
                     IRLS"
     gene            2023..3027
                     /locus_tag="PK754_09145"
     CDS             2023..3027
                     /locus_tag="PK754_09145"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNB09711.1"
                     /translation="MALGNKYRAKISGVAHWVPEQILSNKELEKMVDTTDEWITTRTG
                     IKERHILEKGKATSDLAYEACKRLLEQTNTDPAEIDLIIVGTVTPDMFFPATACLLQA
                     RLGASKAWGYDVSAACCGFAYSLVSGTQFIESGTAKKVIVVGADKMSSIIDYTDRNTC
                     ILFGDGAGAVLLERSEEDTIGILDYYNTVDGNGAQYLNMPGGGSLNPPSHETVDKKMH
                     YVFQDGKNVYQYAVKGMSEASALIVERNGYTGKDVALFVPHQANKRIIDSAANRMGLD
                     ESKVVINIDRFGNTTAGTIPIALSESVSNGRLKKNDLVVIAAFGGGFTCSSILLRWAY
                     "
     gene            3100..4038
                     /gene="fabD"
                     /locus_tag="PK754_09150"
     CDS             3100..4038
                     /gene="fabD"
                     /locus_tag="PK754_09150"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNB09712.1"
                     /translation="MSKIAFVFPGQGSQYVGMGKDLYDLFPTVKAMYETANRIIGPDF
                     SKICFEGPEDALKQTRITQPAIFVHSVALLEVLREKFGTDKVTFHMTAGHSLGEYSAL
                     VAAGVLTFEDALAVVKVRAEAMQRAGEINKGTMAAVIGMEPEPLVSICKTASSEAGVV
                     QCANFNSPGQIVISGSVAGVQKAMEMAKAQGVRMVKELVVSGAFHSPLMEPARAEVQN
                     KLKTITLSDARLPVYANVSAEPTTDAQRVQSLLVEQITGAVRWQESVTRMIADGATKF
                     FEIGPGNVLQGLIKRIDKSVSCECIGKADELAKFVI"
     gene            4075..4827
                     /locus_tag="PK754_09155"
     CDS             4075..4827
                     /locus_tag="PK754_09155"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006838175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HNB09713.1"
                     /translation="MCSLTNRIAVVTGSSRGIGKAIAVEFLKHGASVVINGKTSERIQ
                     QTTDELNALGFSGKFVSCVADVSVATEAEALIRKATEAFGRLDILVNNAGVFKNSLMV
                     RMDEQEWNWVIENNLKSAFFCSKYAAKIMMKQRYGRIIHMSSVVALGGNPFQANYVAS
                     KAGIDGLMMASAKELAPYGITVNSVAPGYIKTDMTKDFSESVVAEILKYIPLGRPGSA
                     EEVAHAVKFLASDEASYMTGQVIQVDGGRVIR"
     gene            4892..5143
                     /gene="acpP"
                     /locus_tag="PK754_09160"
     CDS             4892..5143
                     /gene="acpP"
                     /locus_tag="PK754_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNB09714.1"
                     /translation="MTREEITKKVTEIIVKKLGVTEAEVKPEASYVEDLGADSLDQVE
                     LVMEFEDSFSLGDKIPEDEAAKLTTVGSTIDYLVNRLGK"
     gene            5322..6566
                     /gene="fabF"
                     /locus_tag="PK754_09165"
     CDS             5322..6566
                     /gene="fabF"
                     /locus_tag="PK754_09165"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015173009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNB09715.1"
                     /translation="MSKRRVVITGMGAVTPIGNTVEEFWKGLLEGRNGVGPITLFDAK
                     DHVTKFAAEVKNFDPSLYTDKKEARRMDRFCQFAVAASKMAIEDAKLDVKNLDANRIG
                     CVIGSGIGGFQTFEEQFETYMAKGPGRVSPFFIPMMIIDIAPGQVAIQHGLKGPNYAT
                     VSACATGSHAIGDAFILIQRGDADAMVCGGAEAGICRMGVAGFNALKALSTRNDDPMH
                     ASRPFDKERDGFVIGEGAGIVVLEELEHALKRGAMIYGEMAGIGYTDDAYHITAPAEG
                     GEGAARAMAIACKDAGVKPEEVGYINAHGTSTEYNDKNESAAIKTVFGDHAYKLLVSS
                     TKSMTGHLLGAAGGIEFIATTLAVKNGWIPPTINYEVSDPACDLNYVPNKAIQRNVDV
                     ALSNTFGFGGHNTCIAVTKYKP"
     gene            6572..7390
                     /gene="rnc"
                     /locus_tag="PK754_09170"
     CDS             6572..7390
                     /gene="rnc"
                     /locus_tag="PK754_09170"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNB09716.1"
                     /translation="MSPGTSQEHAVHGGWLNGIIRFFRRAWGPEQADGFLPDNGLRLQ
                     DLSRVLGVRIENPKLYIQALKHRSFLNVSNEKHFSSYERMEFLGDAILSTITAEFLFQ
                     KYPESDEGFLTKGKSHLVNKKTLAHKAEALGLGQFIVMSDGEERSGGRSRSSILSDVF
                     EALIAAVYLDHGYHTARKFVLDTLLCDTERILQSEMNSNFKGDLLEYTQGKDLGIPQY
                     IVVSETGPEHQKEFTIQVAVKDTILGCGRGLSKKEAEQQAAKEALQRIKNNGIQ"
     gene            7406..>8212
                     /locus_tag="PK754_09175"
     CDS             7406..>8212
                     /locus_tag="PK754_09175"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013914708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNB09717.1"
                     /translation="MKKICVVGTGYVGLVSGACLADFGNNVICADIDKEKIDKLNQGV
                     IPIYEPGLKEVVDRNVEKKRLFFSNDVAQSIKESEVIFIAVGTPPGPKGDADLSIVFK
                     VAETIAKNLNGYKVVVTKSTVPAGTGEKVRKILSEHAPKGAEFDVVSNPEFLREGSAV
                     SDFLRPDRVVIGSNSDRATEVMKTVYRALYINETPMVVTNVESSEMIKYASNAFLAVK
                     ISFINEIANICEKIGADVKVVAKAMGLDGRISPKFLHAGGGFGGSCFPKDT"
BASE COUNT         2240 a         1653 c         2211 g         2108 t
ORIGIN      
        1 tcctcttttc ccttgacaat ccaaacaaaa aaaactatct tcgtgcccca aaatttgata
       61 agtgttttat caaattgaag ttaggtgttc gcttggttgc gaaaattgac aaaattttaa
      121 gcttaggaga cggtgaacac cgtttccatt ttgatgttaa ggccgaagat atcggcttga
      181 acagtattga ttttgaaggc cggaatcact tcgaaaaacc gatttcggcg gacgcggtgt
      241 tgaccaaaac caatcacgtc tattatataa agatgaaagt ggttggcgag gctagattgg
      301 tatgcgaccg ttgtttggag gatgtaagtg tagatgtggc cgatgatttt caggtcatct
      361 acaccgatca tcggagcgag cggtacgcgc agagcggtga aacggaaata cggttattgg
      421 atgtccgcaa ggaaaaccaa atcgttttag ataaagatat tgtagatacg attgagcttg
      481 cgatgccgca taaagttctt tgcgatgaag attgtaaagg tttgtgtgta cagtgcggag
      541 cgaatctcaa tgaacgtgca tgtgagcatg cgcagaccgt gaacgaataa cgattttaca
      601 ttattttagg agaatatcat gcctaatccc aaacgcagac attcaaagca gcgccgtgat
      661 aaacgtcgta cgcactggaa actggataat gccgccgtcg gcaaatgtgc caactgtggt
      721 caacccaaaa tgcatcaccg tgtgtgcccc aactgcggac actacgatgg ccgtcagatc
      781 gtgatcgcgc gcgaagcctg atttggagtg cgctcaaatc gtctaagtaa acctgtcctt
      841 gttcccacgg caacgacagg ttttttttta attgcggttt gataatgtgg gttgataacg
      901 gtatattggc gcggtctttt cgtaatagga aacgaacagg catataagtc ggagaaggta
      961 atgaagatcg ttgtggatgc gatgggcggt gatgaagcgc ccaaacatat aattgacggc
     1021 gtcattttgg cggctcgggc atgggtatct accggcgtaa atcacgaaat cattctgacc
     1081 ggcgataaag atgtagtaat gaaagagatc gagcgctgcg gaggtcgtga tttatgcggc
     1141 aatattttac gcttagaaca tacggcatgt gtcatcgata tgcatgagtc actggcagac
     1201 gcttggcgaa ataagtccgg ctcctcgatt catcgcggtg cggaattagt caaatccggc
     1261 caagcggatg cgtttatcgg gatgggcaat accggggcaa tgatgacggc cggattactc
     1321 ggcgttggac ggatcgaagg tgttcaacgt cctacaatag gtgccttttt cccgacagcc
     1381 cgcgggcgct ctctggtgct cgatgtgggc gccgtcacgg attgtaaacc gcaacatctt
     1441 ttgcagtttg gcctcatggg tagtatttat atggaggcga tgtggggtgt aaaaaatccc
     1501 acggtagggc ttttgagtat cggtgaagaa gactccaaag gaaatgaagt aactaaagaa
     1561 accaatgctt tgtttcgtga aaaaaaatta ttcaattttg cgggtaatgt ggaaggtcgg
     1621 gacattctca agggaacgac ggatgtcatc gtgtgcgacg gttttgtagg aaatatcgta
     1681 ttaaaatttg gagaaagcgt tcccggtttt ctcaaagccc gtttcaaaca ggccgctgag
     1741 tcgagtttta tgacgaaact ccaattgctg ttggcaaaga ccggcatcaa gggtgttttc
     1801 aaagatatga attatgaaga atatggcggc gttcctttgt tgggtgtaaa cggcgtggtg
     1861 atcatcggtc acggcagttc ttcgcccaaa gccatgtaca acgcgatcat ggttgcgcaa
     1921 aaaatggtgg ataaaaaaat aaacgaagtt attaaagaac gtattcgatt atcataacta
     1981 acacactgcg cgctgtgctg catgccgcag tagaaaagaa ttatggcgtt aggcaataag
     2041 tatcgtgcaa aaatctcggg tgttgcccac tgggtgcccg agcaaattct cagcaataaa
     2101 gagcttgaga aaatggtaga taccaccgat gaatggataa ccacgcggac cggtatcaaa
     2161 gaacgacata ttttagaaaa aggtaaagcc acatccgatc ttgcctatga agcttgtaaa
     2221 cgccttttgg aacaaacgaa tacagatcct gccgaaattg atttgatcat tgtggggacc
     2281 gttacgccgg atatgttttt tccggcgacc gcatgtcttt tacaggcacg acttggtgct
     2341 tccaaggcct ggggctatga tgtatccgcg gcatgctgcg gctttgctta ctcattggta
     2401 tcgggcactc aatttatcga atccggtacg gccaaaaagg taatcgtcgt gggcgccgat
     2461 aaaatgagtt cgatcattga ttataccgat cgcaatacct gtatattgtt cggcgacggg
     2521 gccggcgccg ttttacttga gcgttctgaa gaagatacga tcggtatttt agattattat
     2581 aacaccgtag atggtaacgg ggcgcagtac ctcaatatgc ccggcggcgg cagccttaat
     2641 ccgccttcgc atgaaacggt tgacaaaaag atgcattatg tttttcaaga cgggaaaaat
     2701 gtatatcaat atgccgtcaa aggtatgagc gaagcgtctg cgttgatcgt cgaacgaaac
     2761 ggttataccg gtaaagacgt agcgcttttt gtgccccatc aagccaataa gcgtattata
     2821 gatagtgcgg ccaatcgtat gggattggat gagagtaaag ttgtgattaa tattgatcgt
     2881 ttcggtaata ccacggccgg caccatcccg attgccttat cggagtcggt cagcaatggg
     2941 cgccttaaga aaaatgactt ggtcgtgatc gctgcatttg gcggcggatt tacatgcagc
     3001 agtattttgt tgcgctgggc gtattaataa ttagcggaat gtgacgaatc cgttggcttg
     3061 tactgtcatg gataaaaata ataaataaat aaaaatcgta tgagtaaaat cgcttttgta
     3121 tttcccgggc agggttcaca gtatgtcggt atgggtaaag atttgtacga tctgtttccg
     3181 accgtcaagg ctatgtatga aacggccaac cggattatcg gtcctgattt ttcgaaaatt
     3241 tgttttgaag ggccggaaga tgcgctgaaa caaacgcgca ttacacaacc ggcgattttt
     3301 gtgcatagcg tggcattact ggaagttctg cgtgagaagt ttggaacgga taaagtgaca
     3361 tttcatatga cggcggggca cagtttgggc gaatattccg ctttggttgc cgccggtgtg
     3421 ttaacctttg aagacgcttt agccgttgtc aaagtacgag ccgaagcgat gcaacgtgcg
     3481 ggtgaaatca ataaaggcac gatggcggcc gttatcggta tggaacctga accgctggtg
     3541 tctatttgca aaaccgccag cagcgaagca ggcgtcgtgc agtgtgccaa ttttaacagc
     3601 cccggacaga ttgtgatttc cggttccgta gccggagtgc agaaagctat ggaaatggcc
     3661 aaggcccaag gcgttcgtat ggtaaaagag ctagtcgtga gcggcgcttt ccattcacct
     3721 ttgatggaac cggcgcgtgc tgaagtgcag aacaaactta aaaccataac actgagtgat
     3781 gcgcgtttac cggtgtatgc caatgtgtcg gcggaaccca cgacggatgc tcagcgtgta
     3841 cagtctttgc tggtagaaca aatcacgggt gcggtgcgct ggcaagagtc cgtaacgcgt
     3901 atgatcgccg acggcgcaac taagtttttt gaaatcggtc ccggtaatgt tttgcaggga
     3961 ttgatcaagc gcattgataa aagtgtatcc tgcgaatgca tcggtaaagc cgatgaatta
     4021 gctaaatttg taatataacg ttgcctttat tgtgcataag caaaggcgaa cgccatgtgt
     4081 tctttgacga atcgaattgc cgtagtgacc ggctcctccc gcggaatcgg taaagcgatc
     4141 gccgtagagt ttttgaaaca cggcgcatcg gttgtgatca acggaaaaac ttccgagcgc
     4201 attcagcaaa cgacggatga attgaatgcc ttgggatttt cgggaaagtt tgtatcctgt
     4261 gtggccgatg tatctgttgc aacggaggcg gaggcgctga tacgcaaggc gacggaggca
     4321 tttggtcggt tggatatttt ggttaacaat gccggagtat ttaagaatag cttgatggtg
     4381 cgcatggatg aacaggaatg gaactgggtc atcgaaaaca atctgaaaag cgcttttttc
     4441 tgttccaaat atgcggcgaa gatcatgatg aagcagcggt acgggcgcat catacatatg
     4501 tcttcagtgg tagcgctcgg cggaaatccg tttcaagcca actatgttgc atccaaagcc
     4561 gggattgacg ggttaatgat ggccagcgca aaggaattgg caccgtacgg cataacggtt
     4621 aattccgttg ctccgggtta tatcaaaacc gatatgacga aagatttttc cgaatcggtc
     4681 gtcgcggaaa tattaaaata cattccgctt ggtcgcccgg gcagcgcaga agaagtcgcc
     4741 catgcagtga agtttttagc gtcggatgaa gcttcctata tgaccggtca ggtcatccaa
     4801 gtggacggcg ggcgtgtaat ccggtaatga tgttggagtg aactttagaa tatataccta
     4861 aacaacaact cttcaaaaaa ggagaaaaac catgacacgt gaagaaatca cgaaaaaagt
     4921 aaccgagatt atcgttaaaa aactcggtgt tacggaagcc gaagtaaagc cggaagcttc
     4981 gtatgtagaa gatctcggtg cggactcgct ggatcaagta gagctggtga tggaatttga
     5041 agattctttc agcctcggcg acaaaatccc ggaagatgaa gccgccaaac tcacgacggt
     5101 gggttcgacg attgattatc ttgtaaatcg tctcggtaaa taagcgaatt attgatgggc
     5161 taatgcgtcg gatattcagt tattcggcgg attagccttt ttttatcaaa cttatatcgt
     5221 atccgcttaa gattcatgcc gatttgtacg gtggcctttt ttgcggtatc acgctacgag
     5281 aggtaatttt ttaacattca tcgccaatgt aaggagcgac tatgagtaaa cgcagagtag
     5341 tgatcaccgg tatgggcgct gtgacgccga tcggcaatac ggttgaagaa ttctggaaag
     5401 gattactgga aggacgcaac ggtgtcggac cgataacact atttgatgcg aaagatcacg
     5461 ttaccaagtt tgcggcagaa gtaaagaatt ttgacccgtc gctctatacg gataaaaaag
     5521 aagcacgccg tatggatcgc ttttgccaat ttgccgtagc ggcatcaaaa atggcgattg
     5581 aagatgccaa gctggatgta aaaaatttgg atgccaatcg tatcggttgt gtgatcggtt
     5641 ccggtattgg cggatttcag acgtttgagg aacagtttga aacgtacatg gccaaagggc
     5701 ccggacgtgt cagcccgttc tttattccaa tgatgatcat agatattgcg ccgggtcaag
     5761 tagccataca gcacggtctc aaggggccga attacgcgac ggtgtcggcg tgtgctacag
     5821 gcagtcatgc catcggtgat gcatttattt tgatccaacg cggtgatgcc gacgcgatgg
     5881 tgtgcggtgg tgcagaggcg ggtatttgtc gtatgggtgt tgcagggttt aatgccctca
     5941 aagcgctgtc aacccgcaat gatgatccga tgcacgcgag ccgtcctttc gacaaagaac
     6001 gcgacggatt tgtgatcggt gaaggtgccg gcatcgtggt gctcgaagaa cttgagcacg
     6061 cactcaaacg cggtgctatg atctacggag aaatggccgg tattggctat accgacgatg
     6121 cgtaccatat cacggcaccg gctgaaggcg gcgaaggtgc tgcacgtgct atggcgattg
     6181 cgtgcaaaga cgcgggtgtg aagccggaag aagtcggata catcaatgcc cacggaacgt
     6241 ccaccgaata taatgataaa aacgaaagtg cagcgatcaa gaccgttttc ggcgatcatg
     6301 cgtataagtt actggtcagt tcgaccaaat ccatgacggg tcacttgctt ggtgcggccg
     6361 gcggcatcga atttatcgcg accacattgg ccgtgaaaaa cgggtggatt ccgccgacga
     6421 tcaattatga agtatcggat ccggcgtgtg acctgaatta cgtgcctaac aaagccattc
     6481 aacgtaacgt ggacgtagcg ctgagtaata cctttgggtt tggcggacac aatacgtgta
     6541 ttgccgtaac gaagtataaa ccgtaatctg tatgtcaccg gggacttcac aagagcatgc
     6601 agtacatggc ggctggctta atgggataat ccgttttttc cggcgcgctt ggggtcccga
     6661 acaagccgat ggttttttac ctgataacgg attacggctt caggatttat cgcgtgtttt
     6721 aggtgttcgg attgagaatc cgaagttata tattcaagca ctcaaacatc gttctttctt
     6781 aaatgtgagt aatgagaaac acttttcgtc gtatgaacgc atggagtttc tcggtgatgc
     6841 gatactcagt acgatcacgg cggaatttct ttttcagaaa taccccgaat ccgatgaagg
     6901 ctttttgacg aaagggaaat cgcacttagt caataaaaag acgttagcgc ataaagccga
     6961 agccttggga ctgggtcagt ttatagtgat gagcgatggt gaagagcgct cgggtggtcg
     7021 ttcgcgttca tctatattat cggatgtttt tgaagcgctc atcgcagcgg tttatctgga
     7081 ccacgggtat catacggcgc gtaaatttgt tttggatacg ctgctttgcg ataccgagcg
     7141 tattttgcag agcgaaatga attccaattt taagggcgat ttactggaat atacccaagg
     7201 caaagatctc ggtattccgc agtatatcgt cgtcagcgaa acagggcccg aacatcaaaa
     7261 agagtttacc attcaagtag ccgttaaaga taccatactt ggatgcggca gagggttatc
     7321 caaaaaagaa gcagaacagc aagccgctaa agaggctttg caaagaataa aaaataacgg
     7381 aatccaataa tctagaggta ttactgtgaa aaagatatgc gtggtaggaa ccggttatgt
     7441 tggcctggta tccggtgcgt gtttagccga ttttggcaac aacgtcatat gcgccgatat
     7501 tgataaagaa aaaattgata aactcaatca aggtgttata ccgatttatg agccgggcct
     7561 taaggaagtg gtggatcgca atgttgagaa aaaacgtttg tttttttcca atgacgtggc
     7621 gcagtctatc aaagagtccg aagtcatttt tatcgcggta ggtacgccac ccggacctaa
     7681 gggggacgcc gatttgagta ttgttttcaa agtcgccgaa actattgcaa aaaacttaaa
     7741 cggctataaa gttgtagtca ccaagagtac ggttcctgcg ggtacgggtg aaaaagtcag
     7801 gaagattctt tccgagcatg cccccaaagg tgccgaattt gacgtggtat ccaatcccga
     7861 atttttacgc gaaggttctg cggtcagtga tttcttaaga ccggatcgtg tggtcatagg
     7921 gagcaacagc gatcgtgcga cagaggtgat gaaaacagtg tatcgggcgc tttatatcaa
     7981 tgagacgccg atggtcgtga ccaatgtcga atcatccgaa atgatcaaat atgcatcgaa
     8041 tgcatttctg gccgtaaaaa tttcgtttat taatgaaatc gccaatattt gcgaaaagat
     8101 cggtgctgat gtaaaggttg ttgccaaagc gatgggattg gatggccgta tcagtccgaa
     8161 atttctccat gccggtggcg gtttcggcgg ttcttgtttt cctaaagata cc
//
LOCUS       DAOQNQ010000132         8183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3247_length_8183_cov_5.981299, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000132 DAOQNQ010000000
VERSION     DAOQNQ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8183
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3247_length_8183_cov_5.981299"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(343..672)
                     /gene="trxA"
                     /locus_tag="PK754_09180"
     CDS             complement(343..672)
                     /gene="trxA"
                     /locus_tag="PK754_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNB09718.1"
                     /translation="MSHPVVLSDASFENEVIKSDKPVLVDFWATWCGPCRMIAPMVEE
                     LAKEYDGKAKICKIDVDANPAVASKFGIMSIPTLLFFKNGQLADKVVGAVPKAKLVEK
                     LNGVMAK"
     gene            complement(733..1269)
                     /gene="lspA"
                     /locus_tag="PK754_09185"
     CDS             complement(733..1269)
                     /gene="lspA"
                     /locus_tag="PK754_09185"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNB09719.1"
                     /translation="MKNYWVAIIIVILDQITKYIVKETMILHDSIPLWDTTIQLTYLE
                     NPGMAFGIRFFEDHPFWGRWFFSIVSIVASGALIAYIHSMRHEKWPYRFSLSLILGGA
                     VGNLIDRVMFGRVVDFMDVDIPDMLGMQRWPVFNVADSAVVLGMIIMTIFVLTHKPAS
                     NTVNETSGTDTPATGGIN"
     gene            complement(1284..1682)
                     /locus_tag="PK754_09190"
     CDS             complement(1284..1682)
                     /locus_tag="PK754_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HNB09720.1"
                     /translation="MKKKDLEYFKKLLQEEINKVLKDMGYIKETLLEETSREAAGDHS
                     GYSFHMADQGSDTHDREKQFMLAARENKYLSDLADAMERVKAGTYGVCMICNKDIEKE
                     RLEMVLVAKYHVACKKKLEEVKQKEHQTES"
     gene            complement(1723..4863)
                     /gene="ileS"
                     /locus_tag="PK754_09195"
     CDS             complement(1723..4863)
                     /gene="ileS"
                     /locus_tag="PK754_09195"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNB09721.1"
                     /translation="MFKEIESQDELAKLSAEVLDFWKKNRIFERSVEERDPKKPFVFF
                     EGPPTANGRPGIHHVLARSMKDFVCRYKTMQGYRVERKGGWDTHGLPVEIEVEKSLKL
                     SGKKDIEAFGMEAFNKKCFESVWTYKELWDQLTDRMGYWVDLKDPYITCDNNYVESVW
                     WILKQYFEKGLVYKGFKVVPYCPKCGTPLSSHEVAQGYQDVSDPSVYVKMKVHGHENT
                     YFLVWTTTPWTLISNVALAVGKDIDYVKAKYKDQFLILAEARVKDVLKEDYEIVERFK
                     GAHILGWEYERLFDDIPVDKKAFFVIPGDFVTTDDGSGIVHTAPAFGEDDYQVCKANG
                     LPFLRPVDAAGKFEETVKEFKGMFVKDADPEIITNLKRRGMLFRSEKFKHSYPHCWRH
                     RTPLIYYARDAWYIRTTTYKDNMVANNETVQWFPPEIKEGRFGNWLRNNIDWSLSRDR
                     YWGTPLPIWVHEESGEMICVGSREELQKGRMKDGSPVPADLDLHKPYVDHVVIEKDGK
                     TFTRTPEVIDVWFDSGAMPYAQWHYPFENKEIFERQYPADFICEGVDQTRGWFYSLMS
                     ISTFLFGKAPYKNVVVNELVLDGQGRKMSKSLGNVVNPFEMLDQYGADILRWYLLVNT
                     PIWLPKRFDLEGLTEMKKKFFRPLLETYKFFTLYANIDGFDPKAASIALSDRTELDRW
                     ILSRLHSTVREVTEHLNQFDTCKAGKLIQEFVDQDLSNWYIRLSRRRFWKGEKGSDKT
                     AAYQTLFEILETLSRLIAPFCPFLAEKMYRHLMQNEDDKTSVHLAYFPEAEKAKMDLA
                     LETKMSSPRRVVQLGRALRELKELKVRQPLAKIIVVTEKESVRGHINDFSDIIKNELN
                     VKAIEFVTDTGSLVNRKAKPNFKALGPKFGKNVNMAAQAIKDFSEKEISLLIEKDHYD
                     LNVAGHEVAITKADVEILHEEKEGLAVQSDADLTVALDTHLTAELVDEGIAREFINRV
                     QNLRKDADFEVTDRIIITLQAEPTILKAIQNQKNYIMNETLATEISVQSPEGEIVRGA
                     ELHDTTLEIGLRRVRG"
     gene            complement(5000..5683)
                     /locus_tag="PK754_09200"
     CDS             complement(5000..5683)
                     /locus_tag="PK754_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017621038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNB09722.1"
                     /translation="MPESFKHIPFRFERKSEPHMIEEARQFYEFMNRRRSVRFFSDEP
                     VPRTCIEYAIRTANTAPSGAHKQPWTWVVIDKPELKRQIREAAEQEERISYEGGRMTE
                     EWLQALAPLGTDWHKPFLETAPYIVVCFRQNYGILPDGRKETHYYVHESVGIACGLFI
                     TAIHKMGLVTLTHTPSPMNFLSKLLNRPENEKPFILFPVGYPAADATVPVLERKPWAE
                     FVQWNDAPV"
     gene            complement(5687..>8183)
                     /locus_tag="PK754_09205"
     CDS             complement(5687..>8183)
                     /locus_tag="PK754_09205"
                     /inference="COORDINATES: protein motif:HMM:NF019935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNB09723.1"
                     /translation="IKKQIVDIKLSVVSQAASPESGFNASQALIELYDSIITEVFARH
                     RLLESDAVCIFALGGYGRREMNLFSDIDINLVYGTTAVPDAIRASVQPFIAQLWDMGL
                     ELGHSVRSIEDCVSLALQDAEIRTSLLDAHYIAGDTVTADKFRQAVQNRIHTHDPQTY
                     IQSRIQHMRARHRHHGNSERVLEPDIKEGKGALRDLHTLYWVIQTAFRISREIETSTS
                     HTIVALRTLGDQHILSSDEIDRTEKAADFLMAVRNALHMFAKRRNDRLSYSVQLQLAT
                     YFGYQDTPEAKGVEVFMRRYYSEARHISHTCITAIHKLHVKFARPAFDDARLPLENGF
                     YLWQHDGDRTLEFNGDFGEHIRTEPRWMIRIFTLAQKYNATLSDTLQATLREHHYMLD
                     EAQITHREIIRDFLEIWRCEGQIASTLRTMHELTLLEKMVPEFGYIVAHYNYNIYHKY
                     TTDEHLIVAVEALERLFFDEVTRDIDIKHLRQIYEELTLDERYQLYWAVFLHDIGKSR
                     GGDHSEIGVTLAGDIFRRLHYTDGVETIQFLILNHLHMEQTAFRRNLKDSETIQNFVT
                     LIGDRRRLRMLYLLTYADMSAANKNVWTEWKSLLLQELFFRTDDALKTHAGESSEFST
                     WEELDYAGIVYDGKLSVSFTDREKYTEVIAVTTDAPYRLATLCGALSVCDVSIIDAQI
                     HTREDGIIVDQFRIFDQWSQKPTTDIQKNKIRSILTEVLEKPEELTAHLHRHRDRWKR
                     KKFIAEQPTEIYFEDNRRFTIIDIFAADRIGLLYTITHALSDLGLNIHSAKIGTRLDG
                     VADCFYVTERDGKKIVSVYRQEEIRSKILNLLR"
BASE COUNT         2074 a         1922 c         1899 g         2288 t
ORIGIN      
        1 ttttgcgaac attgtccacg ggaaaaatag atttttcatc atattctttg gctatcggtt
       61 cgatatattg tttggtaaac gcacgcactt catcgcgaag acggatttct tccggcgaaa
      121 attcaaacgg gaaaaacgac atattccgaa gatcctttca gagagaaaat ttgacgccgt
      181 gaatatagat acggtggtcg gtataatcaa gaagttagcg ggttagtgtt catctaaaaa
      241 caaaagccgt tccatgcgtt gaacggcttt tgtagtttat gaatgtttcg ttacttccgc
      301 atgcgcggtg tagcggtatg ccccgtaggg gcgaaagttt ttttatttgg ccattacacc
      361 gttgagtttt tcaacgagtt tggctttggg gacggccccg actactttat ccgcaagttg
      421 accatttttg aaaaacagca acgtcggaat gctcatgatg ccaaacttgg aagccacggc
      481 gggattggca tccacgtcaa ttttacagat tttagctttg ccgtcgtatt ctttggccaa
      541 ttcttcaacc ataggcgcga tcatacggca cgggccgcac cacgttgccc aaaaatctac
      601 caatacaggt ttatcggatt tgataacttc gttttcaaag ctcgcatcgc tcaatactac
      661 aggatgactc ataatttaac tcctcatttc gctatatttt cagttcgtgc aaatcgtgta
      721 acgtagtaag tttcagttta ttccacccgt agcgggtgta tcggttccgc ttgtttcatt
      781 aaccgtgttc gacgccggct tatgcgtaag cacaaaaatc gtcataatga tcataccgag
      841 aacgacggcg gaatcggcaa cattgaatac cggccagcgt tgcataccca gcatgtccgg
      901 aatatccaca tccatgaagt ccactacgcg tccaaacatc acacggtcaa tcagattgcc
      961 taccgcaccg ccaagaatca gcgagaggga aaagcggtaa ggccattttt cgtgacgcat
     1021 agaatggatg tatgcgatca gcgcgccgga tgcgacgatc gaaacgatcg aaaaaaacca
     1081 acggccccaa aacggatgat cttcaaaaaa acgaataccg aaagccatac cgggattttc
     1141 aagatacgtc agttgtatcg tcgtatccca gagaggaata ctgtcgtgca gtatcatcgt
     1201 ttctttcacg atatacttgg tgatctgatc cagtatgacg atgatgatcg ctacccaata
     1261 gtttttcaaa atcgttatct ttcttaggat tcggtctgat gttctttttg ttttacttct
     1321 tccaatttct tcttgcacgc gacgtgatac ttggcgacaa gtaccatttc gaggcgctcc
     1381 ttttcgatat ctttattgca tatcatacat acgccgtacg tgccggcttt gacgcgctcc
     1441 atcgcatcgg ctaagtcgga caaatattta ttttcacgtg ccgcaagcat gaattgtttt
     1501 tcgcgatcat gcgtatcgct gccttgatcg gccatgtgga acgaataacc cgagtgatcg
     1561 ccggccgctt cgcgcgacgt ttcttcgagc aatgtctcct tgatataacc catatccttc
     1621 aggactttat tgatctcttc ctgaagcagc tttttgaagt attcaaggtc cttcttcttc
     1681 atgataaccc ctctcgttta tgtgaactat ctttttttca atttatccgc gtacacgacg
     1741 caagccgatc tcaagcgtcg tatcatgaag ctccgcaccc cgcacgattt caccctccgg
     1801 tgattgaacg gaaatttcgg tagccaatgt ttcattcata atataatttt tttgattctg
     1861 aattgctttc aaaatcgtcg gttcagcctg tagcgtgatg ataatgcggt cggttacttc
     1921 aaaatccgcg tctttgcgca ggttttgtac gcgattgata aattcacgcg caatgccttc
     1981 atccacgagt tccgccgtta gatgcgtatc cagggctacc gtcagatcgg catccgattg
     2041 gacggccaaa ccttcttttt cttcatgcag gatttcgaca tcggctttgg tgatcgccac
     2101 ttcatgaccg gcaacattca gatcataatg atctttttcg atcaggagtg aaatttcttt
     2161 ttccgaaaaa tctttgatcg cttgtgccgc catattgaca ttcttaccga atttcgggcc
     2221 aagtgcttta aaattgggct tagctttacg attgaccaaa ctgccggtat cggttacaaa
     2281 ttctatagcc ttgacgttga gttcattttt gatgatgtca ctgaaatcat tgatatggcc
     2341 gcgcaccgat tctttttcag tgaccacgat gatcttggcc aaaggctgac gcactttcag
     2401 ttctttgagt tcacgcaagg cgcgtccgag ttgaacgacg cgacgcggag aactcatctt
     2461 ggtttctaaa gccaaatcca ttttggcctt ttccgcttcg ggaaaatagg ccaaatgaac
     2521 gctggttttg tcatcttcgt tttgcatcag gtggcgatac atcttttctg caaggaaagg
     2581 acaaaagggt gcgataaggc ggctcaaggt ttcaagtatt tcaaaaagag tctggtatgc
     2641 cgccgtttta tcgctgcctt tttcgccttt ccagaagcgg cggcgcgaca gacggatgta
     2701 ccaattggat aaatcctgat caacaaattc ctgaatcaat ttaccggctt tgcaggtgtc
     2761 aaattgattc aaatgttccg tcacttcgcg gacagtgctg tgcaggcgcg ataaaatcca
     2821 acgatcaagt tccgtacgat cgctcaacgc gatggatgcg gctttgggat cgaagccgtc
     2881 aatattggca tagagcgtga aaaatttgta cgtctcaagc agcggacgga aaaatttctt
     2941 tttcatttcc gtcaagcctt cgaggtcaaa tcgtttgggg agccagatcg gcgtattgac
     3001 caaaagatac cagcgtaaaa tatcggcgcc gtactgatcc agcatttcaa aaggattgac
     3061 gacattgccg agcgacttac tcattttgcg cccttggccg tcgagcacga gttcgttgac
     3121 tacgacattt ttatacggcg ctttgccgaa taaaaacgta gaaatggaca tcagcgaata
     3181 aaaccaacca cgggtctgat ccacgccttc gcagataaaa tcggcgggat attggcgttc
     3241 aaaaatttct ttattctcaa acggataatg ccactgtgca tagggcattg cacccgagtc
     3301 aaaccatacg tcaatgactt cgggggtgcg ggtaaatgtt ttcccgtctt tctcgatcac
     3361 gacgtgatct acataaggct tatgcaaatc aagatcggcc ggtacgggcg aaccgtcttt
     3421 cattcggcct ttttgcagtt cttctcgact gccgacgcaa atcatttcgc cggactcttc
     3481 gtgtacccat atcggaagcg gggttcccca ataacgatcg cgggataacg accaatcaat
     3541 attattgcgt aaccaattgc cgaagcggcc ttccttgatt tccgggggaa accactgcac
     3601 cgtttcgtta ttggccacca tgttgtcttt ataggtcgtc gtgcggatat accacgcatc
     3661 gcgggcgtag tagatcaacg gtgtacgatg acgccagcag tgcggatagc tgtgtttgaa
     3721 tttttcagag cggaaaagca tgccccgccg tttgagatta gtgatgattt ccgggtcggc
     3781 atctttgaca aacatgcctt tgaattcttt gacggtttct tcgaattttc ctgcggcatc
     3841 caccggacgc agaaaaggca atccgttggc tttacatact tggtaatcgt cttcgccgaa
     3901 agccggcgcc gtatgcacaa tgccggaacc gtcatcggtc gtaacaaaat cgccagggat
     3961 cacaaaaaac gcttttttgt caaccgggat atcatcaaat agacgttcgt attcccagcc
     4021 aagaatgtgt gcgcctttga aacgttcgac aatttcataa tcttctttta gtacatcttt
     4081 gacgcgggct tcggcgagaa tgagaaactg atctttatat ttggctttga cgtaatcaat
     4141 atctttgccg acggcgagcg ctacattgga aataagcgtc catggcgtcg tagtccacac
     4201 caggaaatag gtgttttcat ggccgtgcac tttcattttt acatagacgg agggatcgct
     4261 tacatcttga taaccctgag ccacttcgtg ggatgaaagc ggtgtaccgc atttgggaca
     4321 ataaggaaca actttgaagc ctttgtacac taaacctttt tcaaaatact gcttgagaat
     4381 ccaccacacg gattcgacgt aattattatc acaggtgata tacggatctt tgagatccac
     4441 ccaatagccc atgcggtcgg tcagttggtc ccacaattcc ttatacgtcc agacgctttc
     4501 aaaacatttt ttattgaagg cctccatgcc gaacgcttcg atatcttttt tgccggataa
     4561 tttgagcgat ttttcaactt cgatttcgac gggaagtccg tgcgtatccc agccgccttt
     4621 gcgctcgacg cgataacctt gcatcgtctt gtagcggcaa acaaaatctt tcattgaacg
     4681 tgccagtacg tgatgaatgc cggggcggcc attggccgtc ggaggtcctt cgaaaaacac
     4741 aaatggtttt ttcggatcac gttcttcgac actgcgctca aaaatgcggt tcttcttcca
     4801 gaagtccagc acttcggcgg ataatttggc taattcgtct tgcgattcga tttctttgaa
     4861 catactgagt accgtatttg cacgcgaagc gtaggtgaaa gtcaattttt taaataaatt
     4921 gaggggcata tatataaaaa ggcggggaga aaatcaatca aataaagggc gttttaacat
     4981 cgttttgaat ggatgcaaac tataccgggg catcattcca ttgaacaaat tcagcccatg
     5041 gttttcgttc aagaacaggg acggtggcgt ccgccgcagg gtagcctacg ggaaagagaa
     5101 tgaaaggttt ttcgttttca gggcggttta ggagtttaga taaaaaattc ataggactgg
     5161 gtgtgtgtgt cagtgtgacg agacccatct tatgaatggc cgtgataaaa agtccgcagg
     5221 cgataccgac gctctcgtgg acataataat gcgtttcttt gcggccatcg ggaagtatac
     5281 catagttttg ccgaaaacat acgacgatat acggtgctgt ttcgagaaat ggtttgtgcc
     5341 aatcggtacc aagcggggcc aacgcttgta gccattcttc ggtcattcgt ccgccttcat
     5401 aggatatacg ctcttcttgt tcggcggctt cacggatttg ccttttaagt tccggcttat
     5461 cgataacaac ccatgtccat ggttgtttat gggcgcccga tggtgctgta ttggccgtgc
     5521 gaatagcgta ttcgatacag gtgcgcggaa caggttcatc agaaaaaaaa cgaacgcttc
     5581 ggcgccggtt catgaattcg taaaactgcc gggcttcttc tatcatatgc ggctcggatt
     5641 tacgttcaaa tcgaaacgga atatgtttaa atgattccgg catatttcac ctcagaagat
     5701 ttaatatttt acttcggatt tcttcctgac gataaacgga aacgattttt tttccgtcgc
     5761 gctcggtaac gtaaaaacaa tcggccacac cgtcaagacg cgtaccgatt ttagctgagt
     5821 gaatattcag cccgagatcg gaaagtgcgt gggtgatcgt gtaaagcagt ccgatacgat
     5881 cggcggcaaa aatatcaatg atcgtaaaac gacggttgtc ctcaaaataa atttcagtgg
     5941 gttgttcagc gataaatttt tttcgtttcc agcggtcgcg atgccgatgc aggtgggcgg
     6001 tgagttcttc cggtttttcc aatacttcgg ttaaaatgga acgaatttta tttttctgaa
     6061 tatcggtcgt cggtttttgg gaccattgat caaagatgcg aaactgatcc acgataatac
     6121 cgtcttcgcg ggtatgaatc tgcgcatcta tgatgctgac atcgcatacg ctcagagcgc
     6181 cgcatagtgt cgctaggcgg tagggcgcgt cagtggtgac ggcgatgact tcagtatatt
     6241 tttcgcgatc cgtaaacgaa accgaaagtt tgccgtcgta cacaatgccg gcgtagtcca
     6301 gttcctccca agtcgaaaac tccgaacttt cacccgcatg ggttttgagc gcatcatccg
     6361 tgcggaaaaa aagttcttgt agcaaaagac ttttccattc ggtccataca tttttattgg
     6421 ccgcggacat gtcggcgtac gtcagcagat agagcatgcg caggcgacgc cggtcgccga
     6481 tgagtgttac aaaattttga atggtctccg aatctttgag gttgcggcga aatgccgttt
     6541 gctccatatg caggtgattt aaaatcagaa actgtatggt ttcgacaccg tcggtatagt
     6601 gcaatcgtcg aaaaatatca ccggccagtg tcacgccgat ttcgctatga tcaccgccgc
     6661 gggatttgcc gatatcatgc aaaaacaccg cccagtataa ctggtatcgc tcatccaacg
     6721 tgagctcttc ataaatctgg cgcagatgtt ttatatcaat gtcccgggtt acctcgtcaa
     6781 aaaacaaacg ttccaaagct tccacggcca cgatcagatg ttcgtccgtg gtgtatttat
     6841 gatagatgtt ataattgtaa tgcgcgacga tatagccgaa ttccggtacc attttttcta
     6901 aaagcgtcaa ttcatgcatg gtgcgcaagg tggatgcgat ttgtccttcg cagcgccaga
     6961 tttccaaaaa atcgcggatg atttcgcgat gcgtgatttg tgcttcatcc agcatgtaat
     7021 gatgctcacg taaggtcgct tgcagtgtat cggagagcgt ggcgttgtac ttttgtgcaa
     7081 gggtaaatat ccggatcatc cagcgcggct ccgtacgaat atgttcaccg aaatccccgt
     7141 taaattctaa cgtacggtca ccgtcgtgct gccagagata aaaaccgttt tccaacggaa
     7201 gtcgtgcatc gtcgaaagcg ggtcgggcaa acttcacatg cagcttgtga atggccgtaa
     7261 tgcaagtatg actgatatgg cgcgcctcgc tgtaataccg gcgcataaaa acttcaacac
     7321 ctttggcctc aggggtatct tgatacccaa aatatgtcgc caattgcaat tgtacactgt
     7381 aggataggcg atcgtttctt cgtttggcga acatatgcaa tgcattgcgt acagccatca
     7441 aaaaatcggc ggctttttcc gtccgatcaa tttcatccga agataaaata tgttgatcgc
     7501 ccaaagtacg caaagccacg atggtgtgcg atgtgctcgt ttcgatctct cgtgaaattc
     7561 ggaaagccgt ctggattacc caatacaatg tatgcagatc gcgcagggcg ccttttcctt
     7621 ctttgatatc cggttccaat acacgctctg aattgccatg atgcctgtga cgcgcccgca
     7681 tgtgttgaat gcgggactga atgtacgtct gcggatcatg agtgtgaata cggttttgta
     7741 cggcctgtcg aaacttatcg gccgtgacag tatcgcccgc aatatagtgc gcatcgagca
     7801 gactggtgcg aatctccgca tcttgcaagg ccagcgatac acaatcttcg atcgaacgta
     7861 cactgtgccc taactccaat cccatatccc ataactgcgc gatgaacggt tgcacggatg
     7921 cccgaatcgc atccggaaca gcggtcgtac cgtacaccag attaatatca atgtctgaaa
     7981 aaagattcat ttcccgtcgg ccgtatccgc ccagagcaaa aatgcatacg gcatcgcttt
     8041 ccagaagacg atgccgagcg aatacttctg taataatgct atcgtagagt tcgatgagag
     8101 cttgcgacgc attaaaaccc gattccggcg aagcggcttg cgatacgacc gatagtttga
     8161 tatcaacgat ttgttttttt atc
//
LOCUS       DAOQNQ010000133         8114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3286_length_8114_cov_5.223229, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000133 DAOQNQ010000000
VERSION     DAOQNQ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8114
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3286_length_8114_cov_5.223229"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="PK754_09210"
     CDS             complement(<1..1226)
                     /locus_tag="PK754_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09724.1"
                     /translation="MLRVLLIVFYVGFYSTWAAQSDVLSKTTADSVNYYLLHPVLTNQ
                     AYEVSNYTLTRDRAKFVLKNGFIYTAKPFSGNTIMALFLGTGEFELTPPGDIEQKNLT
                     RFLGTSTLSKEFTNLFFVFTDSTDQWIYRNEPATLTEDEFKNAARFCESAVKYLADPF
                     GEYVDQHTELMNDLVNRRKQGIYLSVFSTLSANDLGSSTTILSKWRDEPLIYKIDPTA
                     NDGEHVTLGRRIRNQGKGFYQEIVSKFPMIPDNSVPYFLRTERKNDFKISDYNMRMTI
                     NENLSSQTSAQLTMRLNNPVNWIRLFLYYRLHVDSVKINGRHVPFHAPVAKDDGFERF
                     ESSPIWINTASVAEQTEPIRVEIFYHGKFLEREGDYVYFKATDDWYPAGFTGRHDRFS
                     FDITYTYPSTYMLVSHG"
     gene            complement(1251..1895)
                     /locus_tag="PK754_09215"
     CDS             complement(1251..1895)
                     /locus_tag="PK754_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB09725.1"
                     /translation="MILQSLTVGPFQENCYIIGDPATREGAVIDPGDEAPRIIKAMEN
                     TGLRFKYIINTHAHIDHVGAVQSVKDHFAIPFYLHRDDEVILRHMPQSAAMFGLKVGR
                     VPDIEFYFNPPADMRLGDLSFKIFHAPGHSPGSVCFYFAMQHILIAGDVLFNGSIGRT
                     DLPGGDFDTLINSIRQNIFTLPEETTVYPGHGPETTVGFEKKFNPFFDHNGLMA"
     gene            complement(1897..2742)
                     /locus_tag="PK754_09220"
     CDS             complement(1897..2742)
                     /locus_tag="PK754_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB09726.1"
                     /translation="MERKNFKIFNKRREPIRGDVCIPDYNRRYPVIVLCHGFKGFKDW
                     GFFPFLSELLCKKGFIVVKFNFSGSGVGEDMQNFTELDRFASNTYSKELEDLERVLDE
                     LEKGNICEKFGYFDKIGLLGHSRGGGITILKAAVDKRIKALVTWSSIATVERHSFMDV
                     LPLWKRQKFIEVPNTRTGQMMRLNLDVVDDIEQNSKTRLNIVKSAARLDAPTMLIHGE
                     NDESVPVNESRQIFDQLHLSRSRIEIIPQATHTYGAVHPFTGPTPELKKAISLTQEWF
                     TNFLK"
     gene            complement(2742..3275)
                     /gene="def"
                     /locus_tag="PK754_09225"
     CDS             complement(2742..3275)
                     /gene="def"
                     /locus_tag="PK754_09225"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNB09727.1"
                     /translation="MATLKIVTYGNPILREKTKPVKKIDATIKTLVRNMIETMHKADG
                     IGLAAPQIGKSLALFVVDISPIEEGHQPMVFMNVEILSSKGNAPYKEGCLSIPGVYAD
                     VHRPEKIKLRYMDMDGKTHEVAADGLLARVIQHENDHINGKLFIDYLDEEMLAPLRPE
                     IEAIEAKYKKPTVAKKK"
     gene            complement(3321..4220)
                     /locus_tag="PK754_09230"
     CDS             complement(3321..4220)
                     /locus_tag="PK754_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09728.1"
                     /translation="MFFPLLESLLHRDIATSKTRIREFLTTDESPRLLYKAFLTIAAQ
                     NAPSDNLHDDAVIKTSAYVAVMEGIDKNKIAHITDALVEYLCTMPIEPIDTSVLKPEF
                     ASHDVVSVHDLEEALDAGDEKLVFEKIRDLLATMDNKQYFMEIMILFGLKRSLNTALA
                     AVHTSMAIQVMDWKNNFTPFLIHRLVRVVCNDKNRETPAKTDRAVDWDDLFRFAPDTY
                     GLIKICALWRSIHEAGVLRSKTEPWVAHYALRLLDTFPKTTDFPVLDQVLSAEDTIRI
                     KGALQGLPHDSARALDSKIPFVV"
     gene            complement(4433..5674)
                     /locus_tag="PK754_09235"
     CDS             complement(4433..5674)
                     /locus_tag="PK754_09235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNB09729.1"
                     /translation="MEGDVWSLDIPGNAGYNNGLYRISSPYCYDKYTSLPPGYEGDGS
                     PYNYMRRFGGTSAACPQVSGVAALILSLNSNFTQSQVQYFIRNSAVDMGTAGWDVDFG
                     FGRLNAHKALVLAGFPIIADPITNIHWCYNSQKKISWTSNLSTSYTVKVSTDGGSTFP
                     TTIASNVTSGSASYSYNWTVNIGPSFTCKLLVIDNNNSALRDTSDLFVIDGTAVPSIP
                     GSFSLTSKGGHPWLTWTTDGSAAGYDLYRASSEYNPPYTWYKFMTINDPTITNYHDVV
                     VDIGITNPNTMKYKINAFNGCNMYSVFTAEKQINYDQFMKNNNANETLIPNEFSLNQN
                     YPNPFNPTTTIEYAVAKTTLVRLTVYDELGREVAILVNEKKMPGIYRVDFDASKFASG
                     VYFYRMTAGEFMETKKLLLVK"
     gene            complement(5681..7084)
                     /locus_tag="PK754_09240"
     CDS             complement(5681..7084)
                     /locus_tag="PK754_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNB09730.1"
                     /translation="MNALYTLMICFLFWSFATAQDLFYYGKGQKIPLSLSDKTLTIKF
                     KTDVSESQMNDLKSSDPDIDKIEKMASRGLHKVSLKNDADIQGIISRLQINPKVERIN
                     PAYKLGEMEMLISDLFRVQFNTGITRAEIDALNEEFGVEIVEELTARPGVFTLRVKSS
                     IASVLDITNRYYEDPNTEWAVPKFFHRWSLHSIPNDTYFASQYYLHNTGQTGGVADAD
                     INAPEAWDITLGSPSIVVAVIDQGVSTGNDPLKPAHEDLPQSRIVRLAGSDFYGSGNG
                     PDENPDPEGNQAHGFAVAGIIAASHNNGTGIAGIAPECKIMPIRMFGARIYDDIYEPL
                     SASDFADAIDFARVNNADVISNSWGNDVDGYWNDDIASAISRALTQGRNGKGCVVVQS
                     AGNNASRSDGYYGFVEFPANVNGVIAVGATDKSNNILYYSPRSKLSQKDSIDVVCPTG
                     QLPSLVLLYNCDNKFIA"
     gene            complement(7140..>8114)
                     /locus_tag="PK754_09245"
     CDS             complement(7140..>8114)
                     /locus_tag="PK754_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09731.1"
                     /translation="IIDGDFWAYLMRTNNTGDTLWMRRYRAPGNLYTSYGLGLAVQQT
                     NDGGFIMVGDTQPDTNKPGDSDFYIVKTDANGDTLWTRFYDRSRTDGATSVKQTSDGG
                     YIISGNSARYLPDGGGVAYVFRLTSDGSWVWGRTIDEYGTTYAVEVNEDGAYMITGDG
                     AIVTKIDPSGNTIWSKFYGRPFGNTLFAIDKTNDGGYVFAGKTEPASGALPVAYLVRT
                     DVKGDTLWTRSYPYLYSGDMRCVSSLPDGGFLMSGNTEKADVIRTDSNGDTLWTRSFG
                     DDLGGAGFIMGKPTEDGGFIFAGGIRLNRRDIYLVKTDSLGFVKSKNQ"
BASE COUNT         2260 a         1846 c         1705 g         2303 t
ORIGIN      
        1 ccatgcgaga ctaacatgta agtggaagga tatgtgtacg taatatcaaa actaaaacga
       61 tcatgacgcc cggtaaatcc ggccggatac caatcatcgg ttgctttgaa ataaacataa
      121 tctccttcac gctctaaaaa ctttccatga taaaaaattt cgacgcgtat cggttctgtt
      181 tgttcggcaa cggaagctgt gtttatccat attggcgaac tttcaaaacg ttcgaatccg
      241 tcatctttag ccacgggagc atgaaaagga acatgacgcc cattgatttt tacggaatct
      301 acgtgcaagc gataatataa aaacaaccga atccagttaa ccggattgtt caatcgcatg
      361 gttaattgcg ccgatgtttg tgatgacaaa ttttcgttaa tcgtcatgcg catgttataa
      421 tcggagattt taaaatcatt tttacgttcc gtacgcaaaa aatacggaac cgagttatcc
      481 ggaatcatag gaaatttact aacgatctcc tgataaaacc ctttcccttg atttcgaatg
      541 cggcgaccga gggtcacatg ttcgccatca ttagccgtag gatcaatttt ataaatcaaa
      601 ggttcatcac gccatttcga caaaatcgta gttgatgatc ctaaatcgtt agcagataac
      661 gtggaaaaaa cggataaata aatcccctgc ttacgtcggt tgacgaggtc attcattagt
      721 tctgtatgct gatctacata ttcgccgaat ggatcggcta aatatttaac ggcggactca
      781 caaaaccgtg ctgcgttttt gaattcatcc tcggttaaag ttgccggttc atttctataa
      841 atccattgat ccgtactgtc cgtaaagaca aaaaatagat tagtaaattc tttgctgagc
      901 gttgatgtac ccagaaatcg agtcaggttt ttttgttcga tgtcgcccgg aggtgtcagt
      961 tcaaattcac cggtacccaa aaacagagcc atgatcgtat ttccggaaaa cggcttcgcc
     1021 gtatagatga aaccgttttt taatacgaac ttcgcacgat cgcgcgttaa ggtgtaatta
     1081 gaaacttcgt aagcctgatt ggttaacacg ggatgaagca gataataatt gacactgtcg
     1141 gctgttgtct ttgataagac atcgctttgt gctgcccacg ttgaataaaa ccctacataa
     1201 aatacgatga gaagtactct taacattgtt ccctcaaact ttgttaaaaa ttaggccata
     1261 aggccgttat gatcaaaaaa aggattaaat tttttttcaa aacccacggt tgtttccggc
     1321 ccatgtcctg gatataccgt cgtttcctca ggaagtgtaa aaatattctg gcggatcgag
     1381 ttgatcagcg tgtcaaaatc gcctccgggc aaatccgttc gcccgatgga accgttaaag
     1441 agtacgtccc cggcgataag tatgtgttgc atcgcaaaat aaaaacaaac actgcccgga
     1501 gagtgaccgg gtgcatgaaa aattttaaac gataaatcac caagcctcat atccgccggt
     1561 ggattaaaat aaaactctat atcaggtacg cgtccgactt tcagaccgaa catagccgcc
     1621 gattggggca tatgcctgag tatgacctca tcgtcgcggt gcagataaaa cggaatagcg
     1681 aaatgatctt tgacactttg tacggcgccg acatgatcta tatgagcatg agtattgatg
     1741 atatacttaa aacgaaggcc tgtattttcc atagccttga tgatacgtgg cgcttcatcg
     1801 cccggatcaa tgacggcgcc ttcgcgcgtt gcgggatcgc cgatgatata acagttttcc
     1861 tgaaaagggc cgacggtgag cgattggagg atcatgttat ttgaggaaat tggtgaacca
     1921 ctcctgcgtg agactaatag cttttttcaa ctcgggcgtc ggacctgtaa aaggatgcac
     1981 cgcgccatag gtatgcgtag cttgcggtat gatttctatg cgagagcggg aaagatgtaa
     2041 ttgatcgaaa atctgacgtg attcattgac cggcacgctt tcatcattct cgccatgaat
     2101 cagcattgta ggtgcatcca aacgagcagc cgatttgact atgttgaggc gcgttttgct
     2161 gttctgctca atatcatcga cgacatccag attaagacgc atcatttgtc cggtgcgtgt
     2221 attgggtact tcgataaatt tctgccgttt ccataacggc aaaacatcca taaacgaatg
     2281 ccgctcaacc gtagcaatgg atgaccaggt aacgagtgct ttgattcgtt tgtccaccgc
     2341 ggcttttaat atggtaatac cgccgccgcg gctatggcca agtaagccga ttttatcaaa
     2401 gtaaccgaat ttttcgcaaa tattgccttt ttccaattcg tccagtacgc gctcaaggtc
     2461 ttccaattct ttggaatagg tattacttgc aaaacgatcc aattccgtaa aattttgcat
     2521 atcttccccg acaccgctgc cggagaaatt gaatttcacg acgataaatc cttttttgca
     2581 aagcaattct gatagaaacg gaaaaaaacc ccagtcttta aagcccttga agccgtggca
     2641 caacacgatc accggataac ggcgattata atccggaata caaacatcgc cgcggatcgg
     2701 ctcgcgccgt ttattaaaaa tcttaaaatt cttacgttcc attatttttt cttcgctacg
     2761 gtaggttttt tatacttcgc ttcgatggct tcgatttccg ggcgcaatgg cgccagcatt
     2821 tcttcgtcca gataatcaat aaacaatttg ccgttaatat gatcgttttc atgctgaatg
     2881 actcgtgcta aaagcccgtc agccgccact tcgtgagttt ttccatccat atccatataa
     2941 cgcaatttga ttttttcagg acgatggacg tcggcataca cgccgggaat gctgagacat
     3001 ccttctttgt acggcgcatt gcctttcgaa ctcaatattt cgacattcat gaataccatc
     3061 ggctgatgac cctcttcgat cggcgaaata tcaaccacaa aaagcgccag cgatttaccg
     3121 atttgcggag cggccagtcc gatcccatct gctttatgca tagtctcgat catattgcga
     3181 accaaagttt ttatcgtcgc atctattttt tttacgggtt tggttttttc ccgtaagatc
     3241 ggattaccgt aggttactat ttttaatgtg gccataaaat tggtatatca tttatacggg
     3301 cggaatgtat aaaaaaacgc ttaaactaca aacggaattt tggaatcaag cgcacgcgcg
     3361 gagtcgtgcg gtaggccttg caatgcacct ttaatgcgaa tcgtgtcctc tgccgacagc
     3421 acttgatcta aaacaggaaa gtccgtggtt ttaggaaacg tatccaaaag ccggagcgca
     3481 tagtgggcaa cccacggctc tgttttactg cggagtacac cggcttcgtg aatactacgc
     3541 cataaggcgc agattttaat aaggccatag gtatccggtg caaatcgaaa taagtcatcc
     3601 caatccacag cacgatctgt ttttgccggc gtttctctgt ttttatcatt gcatacgacg
     3661 cgtaccaagc gatgaatcag aaatggtgta aaattatttt tccaatccat gacctgtatc
     3721 gccatagagg tgtgcacggc tgcaagtgcc gtattcaggg aacgcttgag gccgaaaaga
     3781 atcatgattt ccatgaaata ctgcttatta tccatcgtcg ccagtaagtc gcgtattttt
     3841 tcaaatacga gcttttcatc gccggcatcc aaagcctctt ccaaatcatg caccgatacg
     3901 acatcatggc ttgcaaattc gggttttaat acggatgtgt caataggttc aatcggcatg
     3961 gtacagaggt attccacaag cgcatccgtt atatgggcga tcttattttt atctattcct
     4021 tccataacgg caacgtatgc actggttttg atgacggcgt cgtcgtgcaa attatcggag
     4081 ggcgcgtttt gcgcggcgat cgtcaaaaat gctttatata ataggcgtgg tgattcgtca
     4141 gtagtgagaa attcccgtat acgagttttg gatgttgcaa tgtcacgatg gagcagcgat
     4201 tcaagaagcg gaaaaaacat tattgttaaa tttttttata tctcacacct cgatgatgag
     4261 gtgttcattt tcttttcact taagttaccg tttccagatg attaacgaaa gagccaaatt
     4321 ttaaaaacgc agataaaaac ctgttactcg taaagagtaa caggtttttg ttcataactt
     4381 gatgaagctt agaatttcaa actcagatcg gtttagctcg catcgcgaat acttacttca
     4441 ccaacaaaag tttttttgtt tccataaatt cacctgctgt catacggtag aaatatacac
     4501 cggatgcaaa ttttgaagca tcaaagtcaa cgcgatatat tcccggcatt ttcttctcat
     4561 taaccaatat agccacttcc cgtcccaact catcatatac cgttaatcga acaagcgtcg
     4621 ttttagcaac ggcgtattct atggttgtcg tcggattaaa tggattagga tagttctgat
     4681 ttaaagaaaa ttcattcggt atcagagttt cgtttgcatt attgtttttc atgaactgat
     4741 cataatttat ctgcttctct gctgtgaaca cagaatacat attgcatcca ttaaaggcgt
     4801 ttatcttata tttcatggtg ttggggtttg taatacctat atcaacgaca acatcatgat
     4861 aattggtaat cgtcgggtcg tttatggtca taaacttata ccatgtgtaa ggaggattgt
     4921 attcagacga tgcccgatat aaatcatatc cggccgcaga accgtccgtt gtccatgtaa
     4981 gccaagggtg accaccttta cttgtcaatg aaaatgaacc gggaatagat ggaaccgctg
     5041 tcccatcaat tacaaagaga tcgctggtat ctctcaaagc agagttatta ttgtcaataa
     5101 cgagtagttt acacgtaaaa gatggtccga tattcactgt ccaattgtaa gaatatgaag
     5161 cgcttccgga tgtaacatta gacgctatag tcgtcggaaa agtgctgccg ccgtctgttg
     5221 atactttaac cgtataagat gttgaaagat tacttgtcca tgagattttt ttttgcgaat
     5281 tatagcacca atgaatattg gttataggat ccgctatgat cggaaaaccg gctaaaacta
     5341 aggctttgtg tgcattcaaa cgaccaaacc caaagtcaac gtcccaaccg gctgttccca
     5401 tatctacagc ggaatttctg ataaaatact gaacttgact ttgagtaaaa ttagagttca
     5461 atgacaaaat cagcgcagcc acaccggaaa cctgcggaca agcggctgaa gttccgccaa
     5521 atcgtctcat ataattatac ggagatccgt cgccttcata gcccggagga agcgatgtgt
     5581 atttatcata gcaatacgga gaactgattc tatacagtcc attgttataa cccgcatttc
     5641 cgggtatatc taaactccaa acgtcacctt ccatgccgga tcatgcaatg aatttgttat
     5701 cacaattata gagtaacaca agtgaaggta attgcccggt aggacaaaca acatcaatag
     5761 aatctttttg actgagcttg gaacgcggac tgtaatataa aatattgttt gatttatctg
     5821 tagctcctac ggctatcact ccattgacat tagcagggaa ctctacaaaa ccataatagc
     5881 catccgaacg agacgcatta tttccggcgg actggaccac gacacaccct ttgccattgc
     5941 gaccctgtgt cagcgctcta gaaatcgcgc tggcaatgtc atcattccaa tagccatcca
     6001 catcgtttcc ccagctatta ctgattacgt ccgcgttatt tactcgggca aagtcaatcg
     6061 catcggcaaa atcagaagcg ctaagaggct cgtaaatatc atcataaatc cgcgctccga
     6121 acatgcgaat gggcatgatt ttgcactcag gcgcgatacc ggcgatcccc gttccgttat
     6181 tatggctcgc cgcgataatc cctgccacgg caaatccgtg cgcttgatta ccttcgggat
     6241 caggattttc gtcaggacca tttccgctcc catagaaatc cgagccggct aagcgcacta
     6301 ttcggctctg aggtaaatct tcatgtgcag gtttgagggg atcgttacct gtagaaacac
     6361 cctgatcaat aactgcaacc acgatagagg gactgcccaa cgtaatatcc caggcttccg
     6421 gcgcattaat gtctgcatcg gcgacaccgc cggtctggcc ggtattgtgg agatagtatt
     6481 gactggcaaa ataggtgtca ttgggtatgc tatgcagact ccaccgatga aagaatttcg
     6541 gaacagccca ttctgtatta gggtcttcgt agtatcggtt tgtaatatcc aaaacggaag
     6601 cgatcgaaga tttgacgcgg agcgtaaaga cgccgggccg ggccgtaagt tcctccacga
     6661 tttctacgcc aaattcttca ttaagcgcat ctatttctgc gcgtgtgatg ccggtattga
     6721 actgaactcg aaaaagatct gaaatgagca tctccatttc gccgagtttg taagccgggt
     6781 tgattctttc gactttcgga ttaatttgca gccgcgatat tatcccctga atatcggcgt
     6841 cgtttttcaa cgaaacttta tgtaaaccac gcgatgccat tttctctatt ttatcaatat
     6901 cgggatcgga agacttgaga tcgttcattt ggctttctga cacgtcggtt ttgaatttaa
     6961 tcgtcagtgt tttatctgaa agtgatagag ggattttttg tcccttgcca taataaaaaa
     7021 ggtcttgtgc cgtcgcaaaa gaccaaaaca aaaaacaaat cataagagtg tataacgcat
     7081 tcataaccaa tctccaccgt tagagagtaa tttgtttcag aacagttgat taaaaatact
     7141 tattgattct ttgacttaac aaaccctaaa gaatccgttt ttactaaata aatgtccctt
     7201 ctgttgagtc ttatgccgcc tgcgaaaata aacccgccat cctctgtggg tttacccatg
     7261 ataaatccgg cgccgcctaa atcatcccca aaacttcggg tccacaaggt atccccattt
     7321 gaatcggttc tgatgacatc agctttttca gtatttccgg acataaggaa tcctccatca
     7381 ggcaaactgc ttacacagcg catatcgccg ctatagaggt aaggatatga acgggtccaa
     7441 agcgtatcgc ctttgacatc cgttcgaaca agataggcaa ccggcagggc acccgatgcc
     7501 ggctcggtct ttccggcaaa cacatacccg ccgtcgttgg ttttatcaat ggcaaatagc
     7561 gtattaccga atggccttcc atagaatttt gaccaaattg tattaccgga tggatctatt
     7621 tttgttacga tggcgccatc tcccgttatc atatatgctc cgtcctcatt cacttcgacc
     7681 gcatatgttg ttccatactc gtcaatagtc ctgccccaaa cccacgaacc atccgatgtc
     7741 aatcgaaaca cataagcgac gccgccgcca tcgggcaaat atcgtgcaga attaccggaa
     7801 ataatgtatc ccccgtccga tgtttgtttc accgatgtgg ctccgtctgt ccttgatctg
     7861 tcatagaatc gtgtccagag ggtatcgcca ttggcatcgg tttttacgat gtagaagtca
     7921 ctatccccgg gcttgttcgt atccggttga gtgtctccaa ccatgataaa accgccgtca
     7981 ttagtttgct gaactgcaag ccctagacca taacttgtgt ataggttgcc gggcgcacgg
     8041 taacgtctca tccacagggt gtcgcccgta ttattcgttc gcatcaggta ggcccagaaa
     8101 tctccatcaa tgat
//
LOCUS       DAOQNQ010000134         8100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3292_length_8100_cov_6.201367, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000134 DAOQNQ010000000
VERSION     DAOQNQ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8100
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3292_length_8100_cov_6.201367"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..1389
                     /locus_tag="PK754_09250"
     CDS             229..1389
                     /locus_tag="PK754_09250"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNB09732.1"
                     /translation="MKNRPTNTEPSPTQMDASETLLIEELKSKVSELEASYIRVQDEN
                     QLLHKRLHQTSLLYKIAAMLNSTYDTTKLYNIIQNMLSDISFVRHYALIERTDSGSVN
                     VLFHTHTKDRSLAHVLDAEKEVAEKVFSTEKESYVISVNERKDLLKYFPMKTEGSIVA
                     LPLTFHKKSSKVVCYFAEHSLSDGEKEFLNLITNEIGVAVERALLYQETFEISIKDGL
                     TGIYNRRYFNDRSQREFNRAKRYKHILSFILMDIDHFKKFNDTYGHKVGDEVLVWVAK
                     NLGKGLRDHDVLARYGGEEFIVILPETDVKGAAIVAEKLRKIIDEQSVQINHLFVSQN
                     PHSTFHTDRVTISLGVASFPKDGATLEEVLERTDQQLYVAKSQGRNQVGYEE"
     gene            complement(1451..1523)
                     /locus_tag="PK754_09255"
     tRNA            complement(1451..1523)
                     /locus_tag="PK754_09255"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1488..1490),aa:Lys,seq:ttt)
     gene            complement(1584..2156)
                     /locus_tag="PK754_09260"
     CDS             complement(1584..2156)
                     /locus_tag="PK754_09260"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNB09733.1"
                     /translation="MIIIIDNYDSFTYNLYQYVRELAAPMSDVRVYRHDEITIPQLEA
                     LRPDRIIISPGPGSPHDAGISNDVIRYAADTVPLLGVCLGHQCLAQVYGARILRANEP
                     KHGKVSVIKHDGRGVFAGIPNPLRVTRYHSLIVDPMSLNQKIEISAQTDGGEIMGLRI
                     QGTRAESVQFHPESVMTEHGKKMINNFLKM"
     gene            complement(2258..3514)
                     /gene="metK"
                     /locus_tag="PK754_09265"
     CDS             complement(2258..3514)
                     /gene="metK"
                     /locus_tag="PK754_09265"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNB09734.1"
                     /translation="MSHYLFTSESVSEGHPDKIADQISDALVDHFIAFDPYSKVACET
                     LVTTGQVVLAGEVKSSTYLDVQEITRGVIRKIGYTKSEYMFEAHSCGILSAIHEQSQD
                     INRGVDKKKKEEQGAGDQGMMFGYAVNETADYMPLTLDLAHKLLQELAVLRRENKDIK
                     YLRPDAKSQVTLEYDENRKPVRIDTIVISTQHDDFGSDSAMQDKIRKDMINILIPRVM
                     KRYPKYKHLFNNQIKYFINPTGKFVIGGPHGDTGLTGRKIIVDTYGGRGAHGGGAFSG
                     KDPSKVDRSAAYATRHIAKNLVAAGVVDEVLVQVSYAIGVAKPMGIFVNTFGTGKVGM
                     SDADIAKKVESIFDMRPYFIEKRLKLRTPMYSESAAYGHMGRKNEWVTKTFTSGDGIK
                     KTVKVELFTWEKLDYVGKVKAAFRIK"
     regulatory      complement(3613..3732)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(3807..5204)
                     /locus_tag="PK754_09270"
     CDS             complement(3807..5204)
                     /locus_tag="PK754_09270"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNB09735.1"
                     /translation="MFKGHPRGLKVLFFSEMWERFGIYTMLAIFTLYMGNYFGWPDTK
                     TGQIYGIFLALTYFSPVLGGYLADKFWGFPKTIMVGAIATGIGYAILAIPKPSEPLFY
                     LGLFVVAMSNGLFKANISVLVGNLYEKNSPLKDAGYNIFYMGINVGAFLAPIAATFLR
                     NSIGTEYGYNAGFGAAAAGMLFSFITFRLGQASYNASAAAVAANGSTTMATEERVSPK
                     KEKDRVIALIIVYLIVVFFWMSFHQNGFALTLFAERSTRPMDLPEGLRSVLYVILGIS
                     DPANASEAEFYQVFNPMFILMMTPLVVMFWGWLNKRGLEPATPAKIGIGMMLCGLGFA
                     IMIFASMGGGDADNKSMSPLWLISSYYVQTLAELCLSPMGLSYASKMAPYRWRGLLMG
                     GWFAATAIGNYFAGFLGSFYSTWSHTQFYTVLTVCCFFSAVMIVFFLKKLKHAAEPDE
                     PIENITGEVWGTKPS"
     gene            complement(5294..7258)
                     /locus_tag="PK754_09275"
     CDS             complement(5294..7258)
                     /locus_tag="PK754_09275"
                     /inference="COORDINATES: protein motif:HMM:NF012550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNB09736.1"
                     /translation="MKPFSLGKKRIFYYTEDARSVRDQLEGRSFTYTGQPLVFGVSTD
                     TIINGAACTLGYTEPILGPHFLVGKGDVIHEGDVQKGNFSVVVGGESYGSGSSREVAV
                     VAHRGAGIELVIAKSLERIFRENMTFDRLYFTNDMTVMDKLLAGETVDLDVLMEKQLS
                     PLYKSVFFGGGLLEFGKKVIAGKAELPYKAVRRSVKPMTIAEKILARKAVATDAPGGQ
                     GLNYSEPGEQLFVKADFLGIHEYTGGHVRSLYEKAFGHNPTDKGVTIRGFNDHFVLIG
                     HDDVPGDVKSKRLASAQALAQELIGFCKEHNLPMHGPGGNLPAGVCHRLVVESYAKPG
                     DVIALTDSHTPTAGVMNCFAYGLGSTAIALGLGTGMIPVTVPKTVRIWIEGEDKLNLV
                     TPKDIILHIIGDPYFREMQWKKEPGDTVIMQFGGPALERFTVDELSVLTNMTVEGSAT
                     TGVVEPCTPVIRFLIEKRGMTESDVRSMLVYPDDKATYESVYKVNLADVPVTVATPGD
                     SRNRKPITAVAKTRIDNVVIVSCTGASLEDLKQVAMAIRGHKKLDRIHMVVTPSSQKV
                     YDDAKQLGMIKMFQDFGAVVTKPGCGSCIGNGPGISTKDTITASTSNRNFSGRMGGNG
                     DVFLVSPFVAGVAAVTGELTDPRGWQVVNA"
     gene            complement(7301..>8100)
                     /locus_tag="PK754_09280"
     CDS             complement(7301..>8100)
                     /locus_tag="PK754_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09737.1"
                     /translation="VKQYTNLQEEIELQYKEVVKNPDVTPEEVETAKAEREAQILDAK
                     KDFVYHMPEDYRTWFKKRKVFNSWRHAPEAWGFEESRYWGSRSGLTVREKQILIGGLI
                     IGSVVIGFIVYRIATYTPPTQAVVRELKVEKDSTMNNVDSLMRVKEQQRQQRTITERN
                     NIIINEINNGMYTTQSWNPQDEIRLIMSLYYQYTLYQTIAPADITNEGLKSVSGLIYQ
                     NNPDVFTKFDAGGIPTVWKSPKKIKLNWDEIRTGVERDKKVRAIRRK"
BASE COUNT         2216 a         1999 c         1779 g         2106 t
ORIGIN      
        1 gcgcgcgggc ttcgtgctat tatggaaaaa tttatgaccg acatcatgta tcagataccc
       61 agtatttcgc atttaaaatc agttactatt cacaaagatt gtgtcgtatc gggcggcaaa
      121 ccgcgcatga cgtttgcgga accggaagat tcgaccgtca aaaaaacggc gtaatgttgt
      181 atgcgtagaa agtcatctcc ttcggcttcc atatcaacac aaaaatctat gaaaaatcgc
      241 cccacaaaca ccgaaccatc accgacacaa atggatgctt cggaaacgct tttgatcgag
      301 gaactgaaat ccaaagtttc agaactggaa gcatcgtata tacgcgtcca ggatgaaaat
      361 cagcttctcc acaaacgcct gcaccaaaca agcctgcttt ataaaatagc ggctatgctc
      421 aattcgacgt acgatacgac caaactttat aatatcatcc aaaacatgct gtcggatatt
      481 tcgtttgtcc ggcattacgc cctgatcgag agaaccgatt cgggttcggt aaatgtactt
      541 tttcacaccc acaccaaaga tcgttctctt gcgcatgtgc ttgatgcgga aaaagaagta
      601 gcggaaaaag tgttttcaac ggaaaaagag tcttacgtga tttccgttaa cgaacgaaag
      661 gatttgttaa aatattttcc gatgaaaacc gaaggcagca tcgtcgcact gccgttgacg
      721 tttcacaaaa aatcaagcaa agtggtttgc tatttcgccg agcatagtct atccgacggt
      781 gaaaaagaat ttttgaacct tatcaccaat gaaatcggtg tggcggttga gcgcgctttg
      841 ttgtatcagg aaacttttga aatctccatc aaagacggtc ttacagggat ttacaatcgg
      901 cgctatttca acgaccgttc acagcgtgaa tttaaccgtg ccaaacggta caagcacatc
      961 ctgtcattta ttttgatgga cattgatcac tttaaaaaat ttaacgatac ctacggccac
     1021 aaagtcggag atgaagtact ggtatgggtt gcaaaaaatc ttggaaaagg attacgcgat
     1081 catgatgtgt tggcccgcta tggcggtgag gagtttatag tgatcttacc ggaaaccgat
     1141 gtcaagggtg cagcgatcgt tgccgaaaaa ctacgcaaaa tcatcgatga acaatcggtt
     1201 cagatcaatc atctttttgt gtctcagaat ccgcattcga cctttcatac cgaccgtgtt
     1261 acgatttcac tcggcgttgc gtcttttccc aaagacggcg caacgctcga agaggtactg
     1321 gagcgcacgg atcaacaact gtacgtcgcc aaatcgcaag gacgtaatca ggtgggttac
     1381 gaagaataat acaaatacca aatattgaaa acaaaaatcc cggtcactat gtgatcggga
     1441 ttttgttttg tgagccaggt agggctcgaa cctacgacct tcgccttaaa agggcgctgc
     1501 tctaccgact gagctactag ctcgcctgct tcatttgaag cgtgcaatta tatagaaatt
     1561 acccttagtt ggcaagcaaa aaatcacatt tttaaaaaat tatttatcat tttttttccg
     1621 tgttcggtca tcacggattc gggatgaaac tgcacgcttt ccgctctggt accctgtata
     1681 cgcaagccca tgatttcacc gccatccgtc tgtgctgata tttctatctt ttgattcaga
     1741 ctcatcggat caacgatcag agaatgatac cgtgtaaccc gaagaggatt aggaataccc
     1801 gcaaacacac ctctaccgtc gtgttttatg accgatactt taccatgctt aggttcgttt
     1861 gcgcgtagga tgcgggcgcc atacacttga gccaggcact gatgaccaag gcatacaccg
     1921 agcagaggta cggtatctgc ggcatatcgg atcacgtcat tggaaatacc ggcatcatgc
     1981 ggagaaccgg gtcccggcga tatgatgatg cgatcgggtc gtaatgcctc aagttgagga
     2041 atcgtgattt catcgtggcg atatactcgc acatccgaca tcggtgccgc taattcgcgc
     2101 acgtattgat agaggttgta agtaaaggaa tcgtagttat caataatgat aatcataagc
     2161 caaaattcat aagggctgct ttagcggatt ccgggaagtt ttttcgcttc gaccaaaccc
     2221 ttaaagcagc ccttatatgt aataataacc aaacgtatta tttaattcta aacgcggctt
     2281 tcacttttcc tacgtagtct aatttttccc acgtaaaaag ttcgaccttc accgtttttt
     2341 tgatgccgtc accggaggtg aatgtttttg taacccattc gttcttacga cccatatgtc
     2401 cgtacgcagc cgactccgaa tacatcggcg tgcgcagttt gaggcgtttt tcgataaaat
     2461 aagggcgcat atcaaaaatg gattcgactt ttttagcaat atcggcatcg ctcataccga
     2521 ctttgcctgt accgaaggtg ttgacaaaaa tacccatcgg tttggctaca ccgatagcat
     2581 acgaaacctg caccagtact tcatcaacca caccggcggc aacaagattt ttagcgatat
     2641 gtcgcgtcgc atacgctgcc gaacgatcca ctttggaagg atctttaccg ctgaatgcgc
     2701 cgccaccgtg tgccccacgt ccgccatagg tgtccacgat gattttacga cctgtcaatc
     2761 ccgtatcacc gtgcggaccg ccgatcacaa atttgcccgt cggattgatg aaatatttga
     2821 tctggttatt aaaaagatgt ttgtacttgg gataacgctt cataacacgg gggatcagga
     2881 tattgatcat atctttgcgg attttatcct gcatcgcgct gtccgatccg aaatcatcgt
     2941 gctgcgtgga aataacaatc gtatcaatgc gcacgggctt acggttttca tcgtattcca
     3001 gcgtcacctg ggatttggca tccggacgaa gatatttgat gtccttgttc tcccggcgaa
     3061 gcacagccag ttcttgtaat aacttatggg cgagatccag cgtcagcggc atgtagtccg
     3121 ctgtttcatt gaccgcataa ccgaacatca taccttgatc gccggcgcct tgttcttctt
     3181 ttttcttttt gtcaacacca cgattgatat cctgcgactg ttcgtgaatc gcggaaagaa
     3241 taccacagga gtgcgcttca aacatatatt cactcttggt gtaaccgatc ttacgaataa
     3301 cgccgcgcgt aatttcctgc acgtcaagat acgtactgga tttgacttcg ccggccaata
     3361 cgacctgacc ggtcgtaacc aaggtttcgc acgcgacttt ggaatacgga tcaaatgcga
     3421 taaagtgatc tacgagcgca tcggaaatct gatccgctat tttgtcggga tgtccttcgg
     3481 ataccgattc cgatgtaaac aaataatgtg acatgaatta ctcctgtatt aataaatagg
     3541 ttattaaaat gaacaataaa aaaacctcaa ccgaagtcgg gagaggtttt gaaagaaatc
     3601 tgtgttgctt ccatctcatt gccccgaccg cttacattgc gttcggcagg tattagcacc
     3661 ttgactacgc gcggcggcgt acggttgctg tggcttcatc gtgcctgatc actcggccac
     3721 tcttcataag aaggatatat gtgaaagaac gaatttttta aaagcgggtc aatatatcga
     3781 agcaggaccg cgctgtcaag cagaatttag gatggcttgg tgccccaaac ttcgcccgta
     3841 atattttcga tcggttcgtc aggctctgcc gcatgtttaa gttttttcaa aaagaaaaca
     3901 atcatcacgg ccgagaagaa acaacataca gtcaagacag tataaaactg cgtgtgagac
     3961 caggtactgt aaaacgaacc gagaaatccg gcaaaataat tacctatcgc cgtcgcggcg
     4021 aaccaaccgc ccataagtaa cccgcgccag cggtacgggg ccatcttgct ggcatacgaa
     4081 agccccatcg gactgaggca taattcagct aatgtttgta cataatacga tgaaataagc
     4141 cacagcggag acatggattt attatcggca tcaccaccgc ccatcgaagc aaaaatcatg
     4201 atggcaaaac ccaacccgca gagcatcatg ccgattccga ttttggccgg cgttgcgggc
     4261 tccagcccac gtttattgag ccacccccag aacatgacaa ccagcggggt catcatgagt
     4321 atgaacatcg ggttgaatac ctgataaaat tcagcttccg atgcgttcgc cggatcggat
     4381 atacccaaaa tcacataaag cacgctacgc aagccttcag gcaaatccat cggtcgcgtg
     4441 gagcgttcgg caaataaggt cagtgcaaat ccgttttggt gaaaagacat ccagaaaaaa
     4501 acgacgatca gatatacgat gatcaaagcg atcacacgat ctttttcttt tttgggcgaa
     4561 acacgctcct cggtcgccat cgtcgtactg ccgtttgcgg ctacagcggc agcagacgca
     4621 ttataggaag cctggccaag acgaaatgtg ataaaagaaa aaagcatgcc ggcggcggcg
     4681 gcgccaaatc ccgcattgta accgtattcc gtgccgatcg aatttctaag aaaagtagcc
     4741 gcaatcggag ctaaaaaagc gccgacattg atacccatat aaaaaatgtt ataaccggca
     4801 tctttgagag ggctgttttt ttcatacaaa ttgccgacga gaacggagat attcgctttg
     4861 aaaagaccat tgctcatcgc cacgacaaat aaaccgagat aaaaaagcgg ttcggacggt
     4921 ttgggaatag ccaatatagc ataaccgata ccggtcgcaa tagcgcccac cataatggtt
     4981 ttaggaaagc cccaaaactt atcggcaagg tatccgccaa gtaccggcga aaaataagtc
     5041 agtgcaagga atattccgta aatctgtccc gttttggtgt caggccaacc gaagtaattt
     5101 cccatataca atgtgaaaat cgcaagcatc gtatagatac caaagcgttc ccacatttcc
     5161 gagaagaaca agactttgag gccgcgcgga tgtcctttga acatatctgt tcctttgagg
     5221 aatgatttga aataaaatct gaaaatgtga attgagtatg atcagcgaac catgaaaact
     5281 ttcgatgctt tacttacgca ttcactactt gccatccgcg cggatctgtc aattcgccgg
     5341 tcaccgccgc cacccctgca acgaacggag aaacgagaaa tacatccccg tttccgccca
     5401 tacgaccgct gaaattacga ttgctcgtag atgcagtgat cgtgtcttta gtagaaattc
     5461 ccggcccgtt gccgatacaa gatccgcatc ccggtttcgt tactacggcg ccaaagtcct
     5521 gaaacatctt gatcatacca agttgcttgg catcatcata gactttttga ctgctgggcg
     5581 tcacgaccat gtgaatacga tcaagctttt tgtgaccacg aatggccata gccacttgtt
     5641 tgagatcttc caaagatgca ccggtacaag aaacaataac cacattgtca atacgcgttt
     5701 tggcaacggc cgtaatcggt ttgcgattac gactatcgcc gggtgtggcc accgtaaccg
     5761 gtacatccgc gagattgact ttatacaccg attcgtacgt cgccttatca tcgggataaa
     5821 caagcataga tcgcacatcg gattccgtca tgccgcgttt ttcgatcagg aaacgaatga
     5881 ccggcgtaca aggctcgacg acaccggtgg ttgcgcttcc ttcgaccgtc atattcgtca
     5941 aaacggataa ttcatccacg gtaaagcgtt ccagtgcggg cccgccgaat tgcatgatca
     6001 cggtatcacc cggttctttt ttccactgca tctcgcggaa atacggatca ccgatgatgt
     6061 gaaggataat atctttaggt gttaccagat tgagtttatc ttcaccttcg atccagatgc
     6121 gtacagtttt aggtacggtt accgggatca tgccggtgcc caatcccaac gcgatggctg
     6181 tcgaaccaag tccgtatgca aaacaattca tcacgcccgc cgtcggtgtg tgcgaatccg
     6241 taagcgctat gacatcgccg ggtttggcat aactttcaac aaccaaacgg tgacagacac
     6301 cggcgggaag attgcccccc ggaccgtgca tcggaagatt gtgctctttg caaaatccga
     6361 tcaattcttg cgcgagcgcc tgcgccgaag ccaaacgctt ggattttaca tcgccgggca
     6421 catcgtcatg cccgatcaaa acaaagtgat cgttaaatcc gcgaatagtc acgcctttat
     6481 cggtcggatt gtgaccaaac gctttttcgt acaaactgcg cacatgaccg ccggtgtatt
     6541 catgaatacc gagaaaatcc gctttgacga aaagttgttc accgggttcc gaataattca
     6601 aaccctgtcc tccgggagca tccgtggcta cagctttgcg cgccaatatt ttttcagcaa
     6661 tagtcatcgg ttttacggaa cgcctgacgg ctttataagg caactccgct tttccggcga
     6721 taactttttt tccaaactcc aaaagtccgc cgccaaaaaa tacagattta tacaacggcg
     6781 acaactgctt ttccataagt acatcaagat ctaccgtttc accggcaagc aacttatcca
     6841 tcaccgtcat atcattggta aaatacagac ggtcaaatgt catgttttca cggaaaatac
     6901 gttcgaggct tttggcaatc accagttcga taccggcgcc gcgatgcgcg accacggcca
     6961 cttcacgcga ggaaccgctg ccataacttt cgccgccgac cacgacagaa aaatttcctt
     7021 tttgaacgtc tccttcatgg atgacatcac ctttgccgac caaaaaatgc gggccgagta
     7081 tcggctccgt ataacccaac gtacatgccg caccattgat gatcgtatcg gtactcacgc
     7141 caaaaaccaa cggctggccc gtgtatgtaa acgaacgccc ctcaagctga tcgcgtaccg
     7201 accgggcgtc ttcggtataa taaaaaatac gtttttttcc gagagaaaac ggtttcatcc
     7261 ttcttcctgt atataaatct taatatgaat taattctaac ttatttgcgg cgaattgccc
     7321 ttactttttt gtcgcgctct acgccggtac gaatttcatc ccaattgagt ttgatttttt
     7381 tagggctttt ccaaacggtg gggataccgc ccgcatcgaa tttggtaaat acatccgggt
     7441 tgttttgata tatcaatccc gaaaccgatt tgagaccctc gttagtaata tccgccggag
     7501 ctatggtttg atacaatgta tactgataat acagagacat gatcaggcga atttcgtcct
     7561 gaggattcca cgattgggtc gtgtacattc cgttgttgat ttcattgatg atgatgttat
     7621 tgcgttccgt gatcgtgcgc tgctgacgtt gttgttcttt gacgcgcatc agcgaatcca
     7681 cattattcat cgtcgaatct ttttcaacct tcagctccct taccacagct tgcgtcggcg
     7741 gtgtataggt cgcaattcgg tacacgataa acccgatcac cacactgccg atgatcaatc
     7801 cgccgatgag aatttgtttt tcacgcacgg tcaagccgga gcgacttccc caataacggg
     7861 attcttcaaa tccccaagct tccggcgcat gacgccagga gttaaacact tttcgttttt
     7921 tgaaccaagt ccggtaatct tccggcatgt gatacacaaa atctttttta gcgtcgagga
     7981 tttgcgcctc acgttcggct tttgccgttt ctacttcttc cggagtcaca tcaggattct
     8041 taacgacttc tttgtactga agctcaattt cttcctgcag attggtatat tgtttgacgg
//
LOCUS       DAOQNQ010000135         8062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3311_length_8062_cov_5.929562, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000135 DAOQNQ010000000
VERSION     DAOQNQ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3311_length_8062_cov_5.929562"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..572
                     /locus_tag="PK754_09285"
     CDS             159..572
                     /locus_tag="PK754_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HNB09738.1"
                     /translation="MIRLIVALPVIGGLVYWLNKDESTPDLQGDVKDKNGQPIKASDI
                     DIGSGKICMVNEEKILVINDLERYYALYARCTHQGCIVRWNTKEALVQCGCHGARFDT
                     SGHVLMEPATRPLTAYQVVIKDGFLKLSGKKIIPA"
     gene            complement(559..1608)
                     /gene="ftcD"
                     /locus_tag="PK754_09290"
     CDS             complement(559..1608)
                     /gene="ftcD"
                     /locus_tag="PK754_09290"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNB09739.1"
                     /translation="MSTKPIVECVPNFSEGRDTKVIQTITDAIEKTKGIRLLNVEPDK
                     DYNRVVVTFAGDPQPCVDAALAGMAAACETIDMRRHKGEHPRMGATDVVPFVPVSGVT
                     MDDCAALAYQFGERAARELHVPIYLYAHAARRPERKKLPDIRKGEYEGLREKLTDPMW
                     APDYGRTDYSERVARSGATVTGARFFLVAYNVNVETKDVQQADEIALSVRESGRPKKD
                     AQGNTVKDEKGKVIRIPGSLRETQAKGIWMESFGITQVSMNLLNYLITGMHTAYEECK
                     REAANLGTRVTGSEIVGLIPKDAITMAGRFYADKLKLQISSDDDLIDCAIEQLGLNQL
                     APFDPKKKIIDFMLG"
     gene            complement(1612..2091)
                     /locus_tag="PK754_09295"
     CDS             complement(1612..2091)
                     /locus_tag="PK754_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09740.1"
                     /translation="MIMIDSDIFIMDNFYTNDPRYETNSLFLHRIQVQKNYTTIFNLL
                     EVCSLIAPHCTEKELLGFYENFDRTYNLEIIHPSTYDLSTEYFLDHFFGEIMYMTQNT
                     GNILDAMMFTLVRQRTIRLFVSWNAPRYKKMYAGRFGKLEFLQPDEYMKFYKVTGNR"
     gene            complement(2078..2392)
                     /locus_tag="PK754_09300"
     CDS             complement(2078..2392)
                     /locus_tag="PK754_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09741.1"
                     /translation="MKTVQGVYRNGKVEALEELKADNNTKLLIIVTDEKISDGVAPEL
                     QGVSVLEKIRRRQAADVSTEQQVSSREKLKQVSAKIDGKLPYENIEAASQSLRRYADD
                     HD"
     gene            complement(2421..4058)
                     /locus_tag="PK754_09305"
     CDS             complement(2421..4058)
                     /locus_tag="PK754_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNB09742.1"
                     /translation="MPTKKKTVKKVVRKTKPKAKKATKKTLAKTSARPAKKAKSVKPK
                     PKPAKKVTARKKVKSVSRKVVKKKSAGKKSSVASLKPKSPVFVNPNIFREYDIRGIVA
                     DDLQIEVVELLGKAYGTYMVDKGAKVVTVGRDVRTTSLALKNALVKGMMSTGLEVIDV
                     GEVPTPVLYYSIVFYKTDGGIMVTGSHNPREYNGFKMCLGLAPIFGTEIQRLRHIIDG
                     KAFATGNGTYHEKTIISQYIQMITEKIHLDKKFKIVIDAGNGTAGTIAPKIFRKLGCE
                     VIELYCEPDGTFPNHLPDPTKLEYIKDLQDLVVEHQADLGLGFDGDTDRVGAIDELGR
                     VVWADKLIALMAREINQKNPGQKILFDVKCSQGLIEDIQTHGGVPVMWKTGHSLLKAK
                     MKELHNPLAGEMSGHIFFADDFYGYDDAIYVAARLVQMISRAPHTLSELSDSFPKFYS
                     TPEIRVDTTEADKFRIVDEVRDYFRKKYEIIDVDGVRILYGDGWGLVRASNTQPVLVV
                     RFEAKTETRVHAIMNEVITKLREYPTVQINEADLIIA"
     gene            4182..5552
                     /locus_tag="PK754_09310"
     CDS             4182..5552
                     /locus_tag="PK754_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNB09743.1"
                     /translation="MLPFISINPSNGKKIQSYTETDKDMLNRVIRQSHERFILWSRTS
                     FTERANLLNKISDGLLRERENLAQLMALEMGKPITQGLAEADKCAWVCRYYAENAEAF
                     LKQSDIPTEAKKSYVCFSPLGVVLGIMPWNFPLWQVLRFAAPTWMAGNTIILKHAPNV
                     SGCAEAIARLVSEAGAPQGLYSVIHVQPDLIEDVISNPYIAAVTLTGSTAAGKSVAGL
                     AGRYLKKCVLELGGSDPYIILEDANLETAVDACVTSRLINSGQSCIAAKRFIVTAPVY
                     DNFVEQFVQTMKTKTYGDPQEGNYDIGPIARNDLREKLHSQVLTGKQEGAHVILGGEI
                     PERAGFFYPCTVITDVKKGMALYHEETFGPVATIIRAENETEAIAIANDTMYGLGAAV
                     FTSDVERGEKIAKDHLLAGSCFVNAFVRSDPRLPFGGIKASGLGRELGLYGIREFVNV
                     KTVYVG"
     gene            complement(5613..6512)
                     /gene="yedA"
                     /locus_tag="PK754_09315"
     CDS             complement(5613..6512)
                     /gene="yedA"
                     /locus_tag="PK754_09315"
                     /inference="COORDINATES: protein motif:HMM:NF008432.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HNB09744.1"
                     /translation="MKTLLAFAAIYFIWGSTYLGIKYAIETIPPFLMAGIRNFSPGLF
                     LFIVLKLRGEPSPERSHWRDASIIGALLLLGGNGLVTWAESYVPSGLAALLVATVPLW
                     MSVINGFYGDKLMPTRREIVSILLGLCGLAVLIGPEEIIGGGRIHTLGAFALIAATLL
                     WSFGSLFSRRAHLPSSPMMTVSIEMMVGGGLLLILAVFTSDWHSVQWNAVSQKSILAV
                     IYLCVFGSLLGFTAYIWLLKHVAPNKVATYAYVNPIVAVLLGALMGGEELTVRTLVSA
                     GIIILAVVLMTIKRTTTAQEKKS"
     gene            complement(6552..7160)
                     /locus_tag="PK754_09320"
     CDS             complement(6552..7160)
                     /locus_tag="PK754_09320"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HNB09745.1"
                     /translation="MDYTLFSIFFVTTFLMALSPGPAVFLIMSQGMKFGVRGSMLGAL
                     GIEVMNALYFFISALGLSAALLAFPVIFQVLKWSGIGYLIFMGIKILITAKPRPMQTV
                     QVEADKKLFSQGIITQAANPKAVIYFGSMLPQFIAPDKSIALQFTVLAVVGCVMEFII
                     LSAYGWIAHKGAQFLPGPQAVLWQDRIAGICLLTVALVLITK"
     gene            7361..7777
                     /locus_tag="PK754_09325"
     CDS             7361..7777
                     /locus_tag="PK754_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09746.1"
                     /translation="MLKNSVYSVVIVWFSVFTCLAQEKDPLNLTALMASPNTVYEVRY
                     KDGDGIRKTLCYLRASFKPDHILLYIFDTASDSYKNLEVPKSAIYSVKASNESIGRHA
                     EEEKKQSPFTPKKLIIGGAIVCALIGGIVAVLLLSK"
     gene            7781..>8062
                     /locus_tag="PK754_09330"
     CDS             7781..>8062
                     /locus_tag="PK754_09330"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09747.1"
                     /translation="MIEYKRENLNAFPDEIFSLRDLKILHLHKSHLTELSDRFEFCPQ
                     LAEVSFYQNDLAKIPDSIFKLKNLQRLNFGDNRISVLSYMIGELSCLEML"
BASE COUNT         2270 a         1766 c         1822 g         2204 t
ORIGIN      
        1 atgcccttgt cacggtagca cttacgatat ggacggaaaa gtgcaaaaag gaccttccac
       61 aaccgacatt ctaaaataca aatcggaaag aaaaggcgat aaacttatcg tatatgctta
      121 gaaaaccaac acaaggtcgc cgaagatttg taaaagatat gatacggttg atcgtagcgt
      181 taccggtgat cggaggactg gtgtactggc ttaataaaga tgaatcaaca ccggatttgc
      241 aaggcgatgt aaaagataaa aacggtcaac cgataaaagc ttcggatata gatatcggaa
      301 gcggtaaaat ttgcatggtc aacgaagaaa agatattagt gatcaacgat ttggaacgtt
      361 actatgcgct ttatgcacgc tgtacacatc aaggatgtat cgtccggtgg aatactaaag
      421 aagcattagt gcaatgcggt tgccacggcg ctcgatttga tacatcgggt catgtgctga
      481 tggagccggc cacgcgacct cttacagcat atcaagtcgt aatcaaagac ggatttctga
      541 aattatccgg taaaaaaatc atcccagcat aaaatcaata atcttcttct tgggatcaaa
      601 aggagccagt tggttgagtc ccagctgttc gatcgcacaa tctatgagat cgtcatcact
      661 gctgatttgt aactttaatt tatccgcata aaatcgtccg gccatcgtaa ttgcatcttt
      721 aggtatcaat cctacaattt cacttcccgt tacacgtgta ccaaggttcg cggcttccct
      781 tttacattct tcataggccg tatgcatgcc ggtaataagg taattgagta aattcatgga
      841 gacttgtgtg atcccaaaag actccatcca tataccttta gcttgagttt cacgtaatga
      901 gcccgggatg cggataactt tacctttttc atccttcacc gtattgcctt gtgcatcctt
      961 ttttgggcgt ccgctttcac gcacactcag cgcaatttca tcagcttgct ggacatcttt
     1021 ggtttctaca ttgacattat aagcgaccag aaaaaaacgg gcaccggtaa ccgtagctcc
     1081 ggagcgagca acgcgttcag aataatctgt acggccataa tcgggagccc acattgggtc
     1141 ggttaatttt tcacgtaaac cctcgtattc acctttgcgg atgtccggta gttttttacg
     1201 ttccgggcga cgtgcggcat gtgcgtacaa atatataggc acatgaagtt cgcgagcagc
     1261 acgttcgcca aattgatatg cgagggcggc gcaatcatcc atggtaacac cgctgaccgg
     1321 aacaaaaggc accacatccg tcgcgcccat acgcgggtgc tcacccttat gccggcgcat
     1381 gtcgatcgtt tcacaagcgg cggccatacc ggctagagcc gcatccacac agggttgcgg
     1441 gtcacctgca aaggttacga ccacgcgatt gtaatcttta tcaggttcga cattaagcaa
     1501 gcggatgcct tttgttttct cgatggcgtc cgtaatggtt tgtattactt tggtatcgcg
     1561 gccttcgctg aaattaggca cgcattcgac tatcggtttg gttgacataa gctatcggtt
     1621 tcctgtgact ttgtagaact tcatatattc gtccggttgc aaaaattcca attttccgaa
     1681 acgaccggca tacatttttt tataacgcgg ggcattccat gaaacaaaaa gacgtatcgt
     1741 acgctgacgc accaatgtaa acatcatggc atccaatatg tttcccgtat tttgggtcat
     1801 gtacatgatt tcaccgaaaa aatggtctaa aaaatattcg gttgaaagat cgtaagtgct
     1861 tgggtgaata atttccaagt tatacgtccg atcaaaattt tcataaaaac ctaaaagttc
     1921 tttttcggta cagtgcggtg cgatcaagct gcagacttcg agcagattga aaatcgtagt
     1981 atagtttttt tggacttgaa tgcggtggag aaataaacta ttggtctcgt atcgtggatc
     2041 attggtataa aaattatcca tgataaagat atcgctgtca atcatgatca tcggcgtatc
     2101 tcctcaggct ttgggatgcc gcttcgatat tttcgtaggg cagttttccg tctattttgg
     2161 ccgagacctg ttttagtttc tcacgtgaac taacttgttg ttcggtagag acatccgctg
     2221 cttgacggcg tcttattttt tccaatacgc taacaccttg caattccggt gcgacgccat
     2281 cggagatttt ttcatccgtt acgatgatga gcagttttgt attgttgtcg gctttgagtt
     2341 cctccaaagc ctccactttt ccgtttctat aaacgccttg tacggttttc actcggtgta
     2401 ctccggttac atacgtcgta tcatgcgatg atcagatcgg cttcattgat ttgtaccgtc
     2461 ggatattcgc gtaatttggt tatcacctca ttcataatgg catgaacgcg ggtttcggtt
     2521 ttggcttcga atcgtaccac aagaaccggt tgtgtattgg atgcacgcac aagaccccaa
     2581 ccatcgccat ataaaattcg tacaccatct acgtcaatga tttcatattt cttacggaaa
     2641 taatctcgga cttcatccac aatgcgaaat ttatccgcct cggtggtgtc cacgcgaatt
     2701 tcaggcgttg aataaaattt aggaaaacta tctgaaagct cagatagggt atgcggtgca
     2761 cggcttatca tctgaaccag gcgtgcggcc acataaatcg cgtcatcata cccataaaag
     2821 tcatcagcaa agaaaatatg cccgctcatt tcaccggcga gcggattatg gagttctttc
     2881 atcttagctt taagcagcga atggccggtt ttccacatta ccggaacacc gccatgggtt
     2941 tgaatatctt cgatcagacc ttgcgagcat ttgacatcaa aaagaatttt ctgaccgggg
     3001 tttttctgat tgatttcgcg ggccatcaaa gcgatcagct tgtccgccca aacgacgcgg
     3061 ccgagttcgt caatagcgcc tacacggtcc gtatcgccgt caaatccaag gccaagatcg
     3121 gcttgatgtt cgaccacgag atcctgaagg tctttgatat actccagttt ggtcggatca
     3181 ggaaggtgat tggggaatgt accgtccggt tcgcaataca attcgatcac ttcacaaccc
     3241 agtttgcgaa aaattttggg tgcgatcgtg ccagccgtac cattgccggc atcaataacg
     3301 attttaaatt tcttatccag atgaattttt tcggtaatca tctgaatgta ttgcgaaatg
     3361 atcgtttttt catgataggt gccgttgccg gtagcaaaag ctttgccgtc aatgatgtgg
     3421 cggaggcgct gaatctctgt gccaaaaata ggagccaagc cgaggcacat tttgaacccg
     3481 ttgtattcac gcggattgtg gctgccggtg accatgatgc cgccgtcggt tttataaaaa
     3541 acaatcgagt aataaagtac gggtgtgggc acttcaccga cgtcaatcac ttcaagtcct
     3601 gtggacatca tacctttgac taatgcattt ttgagcgcga gagacgtagt ccggacatcc
     3661 cgtccgacgg ttacaacttt ggcgccttta tccaccatat acgtgccgta tgctttaccg
     3721 agtaattcga cgacttcgat ctgtaagtca tcggcgacaa tgccgcgtat gtcgtattca
     3781 cggaatatat taggatttac aaaaacaggt gatttgggtt taagcgaagc gacggatgat
     3841 ttttttccgg cgctcttctt ttttacaact ttgcgggaaa ccgatttaac cttcttacgc
     3901 gccgtaactt ttttggccgg tttaggcttt ggttttacgg atttcgcttt ttttgcagga
     3961 cgagcagatg ttttagctaa agtttttttg gtcgcttttt tggctttggg tttggtcttg
     4021 cgaactactt tttttacggt tttctttttg gtcggcatga tggatctccg gtgcgtaggc
     4081 tagtatttat aacggcagta aattgtttgt ttagtgagaa aaaagcctta aaatttaaag
     4141 ctgaatgacg gctttatcaa gaactaaaac acacaaaaaa catgttacca tttatcagta
     4201 tcaatccatc caacggtaaa aaaatacaaa gttatacaga aacagataag gacatgctca
     4261 acagggtgat ccgccagtct catgagcgat ttattttgtg gtcccgaaca agttttaccg
     4321 agcgcgcgaa tctgttgaat aaaatttcag acgggctgct tcgtgagagg gaaaatttgg
     4381 cacagctgat ggccttggaa atgggtaagc cgataacgca ggggttagcc gaagccgaca
     4441 aatgtgcatg ggtttgcagg tattatgccg aaaacgcaga agcttttctc aaacaaagcg
     4501 atattcccac ggaggcgaaa aaaagttacg tttgtttttc tccattaggc gtggtactgg
     4561 gcattatgcc gtggaatttt ccgttgtggc aagtgttgcg atttgcagcg ccgacatgga
     4621 tggcgggtaa cacgatcata ctcaaacatg caccgaatgt ttcgggatgt gcggaggcca
     4681 ttgcccgatt ggtatccgaa gccggcgcac cgcaaggatt gtactccgtg atacatgttc
     4741 agcctgatct gatcgaagat gttatttcta atccttatat cgcagcggtg acacttaccg
     4801 gcagtacagc cgcgggaaag tcggtcgcgg gtttggccgg gcgatattta aaaaaatgcg
     4861 tacttgagct tggaggaagc gatccgtata tcattttaga agatgcgaat ttggaaacgg
     4921 ccgtggacgc ttgtgtcact tcacgcctca tcaatagcgg tcaaagttgc atcgcggcca
     4981 aacgatttat cgtcacagca cctgtctatg ataattttgt ggagcaattt gtgcagacga
     5041 tgaaaacaaa aacgtatggt gatccgcagg agggtaatta cgatatcggg ccgatcgcgc
     5101 gaaacgattt acgtgaaaaa cttcattcgc aagttctaac aggaaaacaa gaaggcgccc
     5161 atgtaatact aggtggtgag ataccggaaa gagcgggttt cttttatcct tgtacggtaa
     5221 ttaccgatgt aaaaaaagga atggctttat atcatgaaga gacgtttgga cctgtggcta
     5281 ctataatccg tgcggaaaat gaaacagaag ctatcgcgat agccaatgat acgatgtacg
     5341 ggcttggcgc agccgtattt acttccgacg tcgagcgcgg tgaaaaaata gccaaggatc
     5401 atcttttagc cggttcgtgt ttcgttaatg cgtttgtgcg ctctgatccg agattaccat
     5461 ttggcggcat caaagcatcc ggattgggac gagaattggg tttgtatggc atacgcgaat
     5521 ttgtaaacgt taaaacagta tatgtaggct gagtatggtg ttgaacaata ttaatgccat
     5581 cgcgctcgga acgaaatatt tttttggtaa aactaactct ttttttcttg tgctgttgtt
     5641 gttcgtttga tcgtcatcaa aactacggcc aaaataataa taccggcaga taccaaagtt
     5701 cgcacggtca attcttcacc acccatcaag gcacccagta aaacggctac gatggggttg
     5761 acatacgcat acgttgctac tttattggga gcaacgtgtt tgagaagcca aatatacgcc
     5821 gtaaatccaa gcagcgatcc aaaaacacac aaataaataa cggcaagaat ggacttttgc
     5881 gatacggcgt tccattgtac ggaatgccag tcgctggtaa atacggcaag aatgagcaat
     5941 aacccgccgc cgaccatcat ctctatagac acagtcatca tcggtgagct tggcaaatga
     6001 gcacgtcgtg aaaaaagcga accaaatgac caaaggagcg tggctgcaat caaagcgaat
     6061 gcgcctaagg tgtggattct accgccgcca ataatttcct caggtccgat caacacagct
     6121 aaaccgcata aaccaagaag aatacttacg atctcacggc gcgtaggcat tagcttatca
     6181 ccgtaaaatc cattgatcac ggacatccat agtggcacgg ttgctacaag caaagcggct
     6241 aagccggacg gtacatacga ttcggcccag gttacaagac catttccgcc aagcagaagc
     6301 aaagcgccga tgatcgaggc gtcacgccag tgggaacgtt ccggtgaggg ttcaccgcga
     6361 agttttaata cgatgaacaa aaataaaccc ggtgaaaaat ttcgaatacc ggccatcaaa
     6421 aatggcggga tagtttcgat cgcatacttg atgccgagat acgtcgaacc ccaaatgaaa
     6481 taaatagccg caaaggccaa aagcgttttc agacgatgtg atgaatttcc gatgatgatc
     6541 tccgataagg tttatttggt aataagaacc aacgctacgg tcagtaaaca aattcccgca
     6601 atgcgatctt gccagagtac ggcttggggt ccgggtaaaa attgtgcacc tttatgagca
     6661 atccatccat acgccgacag gatgataaac tccatgacac aacctacaac ggctaaaacg
     6721 gtaaattgta aagcgatgga tttgtccgga gcgataaatt gcggtaacat ggatccaaaa
     6781 taaatcaccg ccttgggatt ggccgcttgt gtgatgatgc cttggctgaa taatttttta
     6841 tcagcttcaa cttggaccgt ttgcataggg cgaggtttgg ccgtgatcaa tattttgatg
     6901 cccataaata tgagatatcc gatgccgctc cattttagaa cctgaaatat aactggaaaa
     6961 gcaagcaaag cggcgcttag accaagtgcc gaaataaaaa aatacaacgc attcattacc
     7021 tcaataccca gcgcaccgag catgcttccg cgcacgccaa acttcatgcc ctgactcata
     7081 atgagaaaaa ccgccggacc cggcgataac gccatcaaaa aagtagtgac aaaaaaaatc
     7141 gaaaatagag tgtaatccat gcagggcttt ttaaattttt ttttaaaatc aaagataagc
     7201 cgaatgaata accggcacaa gtaccattct acgtcaaaag atcatagtcc attgatcact
     7261 ggattcatca tagtgcattt catcggttat accaacccga aacttgctat tgtcttaacg
     7321 aatccacaca ttcattatat cacttataat ggagcgagtt atgctgaaga attctgttta
     7381 cagcgtggtg attgtgtggt tttcggtatt tacctgtttg gcgcaggaaa aagatcccct
     7441 aaatctgacg gcgttgatgg catcacccaa tacagtatat gaagtacgat ataaagacgg
     7501 cgatggtata cgcaaaactc tatgttattt gcgagcatca ttcaagccgg atcatatttt
     7561 actatacatc ttcgatacgg cttcggacag ttataaaaat ctagaagttc ctaaatctgc
     7621 catctacagc gtcaaagcat ctaatgaaag tataggacgt cacgctgaag aagaaaaaaa
     7681 gcaatcacca tttacaccca aaaaactgat catcggcggt gcgatcgttt gtgccttgat
     7741 cggggggatc gtcgctgtat tacttttgtc caaataaaca atgatcgaat ataagcgcga
     7801 aaacctgaac gcgtttccgg atgaaatttt ttcactccgc gatctcaaaa ttttacatct
     7861 tcataaaagc catcttactg agctatctga tcgttttgaa ttctgccctc agctcgccga
     7921 ggtgagcttt tatcaaaacg atttagctaa aattcccgac agtattttta agcttaaaaa
     7981 cttacaaaga ctcaattttg gtgataatcg tattagcgtt ctctcctata tgatcggtga
     8041 actttcctgc cttgaaatgc tt
//
LOCUS       DAOQNQ010000136         7980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3359_length_7980_cov_5.214006, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000136 DAOQNQ010000000
VERSION     DAOQNQ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7980
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3359_length_7980_cov_5.214006"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..713)
                     /locus_tag="PK754_09335"
     CDS             complement(42..713)
                     /locus_tag="PK754_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB09748.1"
                     /translation="MRILLIEDDRKVASFIQRGLSEAGNAVDIVYDGRSGQTQAEVEN
                     YDLLIVDWMLPNKDGVSVCRDLRANGYTMPILLLTARETVNDRVTGLDAGADDYLTKP
                     FAFNELLARARALMRRGSRTLDLFTVDDLEMNLSERRVTRGGQWIELSNREFSILEYL
                     LRNKNRLVTRTSLTEHVWDIHFDRGTNIVDVYINYLRKKIDTGTRKPLIHTIRGAGYM
                     LKEPD"
     gene            987..1466
                     /locus_tag="PK754_09340"
     CDS             987..1466
                     /locus_tag="PK754_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09749.1"
                     /translation="MNPAHMHLLLNHIPVLALAAATILLIAARWRSQIEWTRLSMLFF
                     VIAGAVSVPVFMTGEPAEKIIENIGGISMMHLESHEDSAVASLIGACILGVLAVIGLF
                     FIERVHAHRLIWATFLIIALTANGLMAWTASLGGKIHHTEIHNGYTPAQTQKKYDDD"
     gene            1492..2463
                     /locus_tag="PK754_09345"
     CDS             1492..2463
                     /locus_tag="PK754_09345"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNB09750.1"
                     /translation="MKRWIVYLLLCAGFYFLPVYGQENNEKDDARPSVSGYAQIIYSQ
                     YLKNQEHDRQSGFDINRVRFAFEGSVIPDRVHYAVEFDPQGSPILDDTYLDFIISKNS
                     KVRLGRYKIPFGAENPTGEDELHFIEKSKVVESVFSAREVGIGTYYERNWIIGDVGYF
                     QKSEDEHIERDVIGRIGLKPFGKYIETGGSGYYEKIHTDSTNFAIHRIGADLTCEVGP
                     IKMQGEYIRSKGTMEDKQLSGYGYYGYGQYTHHIGDTQISWGVRYEFYQPDKNNKSDQ
                     ERKYVVGSTIAFHPRMRAQVNFQHIRENIETNNQGQNKLIVQYQFSF"
     gene            2479..3576
                     /locus_tag="PK754_09350"
     CDS             2479..3576
                     /locus_tag="PK754_09350"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNB09751.1"
                     /translation="MFKKYSVLTAACIFGIGTFSVYLFGEWHEIQDEGLRWFLELPHM
                     TLSFFLIIMLPLAVHQMAILIERKNETRMLWTRFMVVVVLPYIVVATYLAAEWDEIEE
                     EWLPWLIKPSHFLIVLINGIMIPGILWLIINKFIQLWDERERFLKRIVELENELRSFV
                     HPEVYKRIYEKTSLEATGAFKIIMADMQGFTRFCEAHSAAEVANLANEFCNLMDERIR
                     QVGGITLSYAGDLVFALFDEHIASDDIVGVAKKMQETFRAFAQTKFPDWDTNLNIGIH
                     YSRDIVLGTIGGGSAQKHFTANGFGICEVSRMCSEADHGQILVSEAFAETVTSDPIRS
                     RLIGIAWSKQPKNITVPPVMFEYRLVDNEIT"
     gene            complement(3614..4120)
                     /locus_tag="PK754_09355"
     CDS             complement(3614..4120)
                     /locus_tag="PK754_09355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNB09752.1"
                     /translation="MKEYNVITTSASVVVIGIGNEYRRDDRAGIEVVRAIADMCLPDV
                     CVIEHNGDGSSLQDVWSDARWVIIIDTVCSDNIPGTIYRLRANADIIPGNFFNYSSHA
                     YSISEAIELAKALHHPVPEVIIYGIEGANFEFGEELTPNVREKIGDLITRVCQDIERI
                     MQESPVSA"
     gene            complement(4161..4619)
                     /locus_tag="PK754_09360"
     CDS             complement(4161..4619)
                     /locus_tag="PK754_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNB09753.1"
                     /translation="MKDLKFFLMQHPLLIDMSAEHVELIIGCASNVRFDTGQIIFREG
                     QEANEFYLIRQGKIALEIGSPGRGHIGIHTLTEGDILGWSWLIPPYHWQFDARALDLT
                     LAIALDGRCLRNKCESDHELGYQLLKRFSHIVTQRLESTRLQILDMIEHV"
     gene            complement(4936..5286)
                     /locus_tag="PK754_09365"
     CDS             complement(4936..5286)
                     /locus_tag="PK754_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010522616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HNB09754.1"
                     /translation="MNLETIRTYCLKKKSTDEDFPFDQETLVFRVSGKIFALINTHIA
                     EFINLKADPEKAIEWRERYEGVQPGYHMNKKLWNSVYLDGSVQDKEIFLMIDHSYDEV
                     VKKLPKKERIKLQP"
     gene            complement(5982..6920)
                     /locus_tag="PK754_09370"
     CDS             complement(5982..6920)
                     /locus_tag="PK754_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNB09755.1"
                     /translation="MNAGITKARVDSLNPRKPLYVEKDATVADVVAMMEQTKSGCILV
                     RDKGALIGTLSERTILRNLIAMHMEFSHTPVTRLMSADTHCLYADDSAEYALNEMTFG
                     GFRNITVLSQVGDPSGILTFEDILDHILAKMHVRIDTEEKKPRSPEKKRAREKQGIDP
                     EDFDRPIGVLDPPAALCVESGSLLKDAVRQMADRNIGCVMVVRDKKLTGTVTERFLLY
                     KIAHETPDFDTAKVDDYMRVAPQCLQMHDPVSAAIRILALHDVRHIPIVNENGEPVAF
                     TSVRGVIDYIVSFYPDEIINLPPHPMRFGLRDEAGA"
     gene            complement(7001..>7980)
                     /locus_tag="PK754_09375"
     CDS             complement(7001..>7980)
                     /locus_tag="PK754_09375"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNB09756.1"
                     /translation="YMHRRQFLKTSGAMLLVCAGAGLGPLFLSRRRVYTMTRPLMGTI
                     GEIHVVSDDARWADRAMEAAFDELQRLESLLTYFSPVSDVGRINAATTGAEIRVASET
                     ADLIERALHWSKVTDGGFEPGLGKVSDTWDVKHRTEPPAQDVWQRFAGLSLHRFIHVR
                     TGSDTTVKILSPDVRLDLGGIAKGYAADRAAEVLKKEGITQALINLGGDIVALGRRAD
                     GEGWRVGVRDPERADVMATVVTLHDQAIATSGTYEQYFRSGGRVYHHLIDPKLAEPKR
                     AQFQSLTIIGTNGRDTDALATGLFFSTDEDKKKILADHTDRFQCMTWGA"
BASE COUNT         2072 a         1860 c         1941 g         2107 t
ORIGIN      
        1 ataaagccga gtatgaattt ccatcgaagc gatacgcgca tctaatccgg ctccttgagc
       61 atatatccgg caccgcgaat ggtgtgaatc aaaggcttac gggttcccgt atcaattttt
      121 ttgcgaagat agttgatata gacgtccacg atattggttc cccggtcaaa gtggatatcc
      181 catacgtgct cggtcagtga agtgcgggtc acaaggcgat ttttgttacg cagtagatac
      241 tcaagtatcg aaaattcacg attggataat tcgatccatt gtccgccgcg ggtaacgcgc
      301 cgttcggaaa gattcatttc cagatcatcc acggtaaaaa gatccagcgt acgcgatcca
      361 cggcgcatga gcgcgcgcgc gcgtgctaga agttcgttga acgcaaacgg tttggtcaag
      421 tagtcatcgg cgccggcatc caaaccggta acacgatcgt tgacggtttc gcgcgctgta
      481 agcaggagta tgggcatcgt gtaaccattg gcgcgtaagt cgcggcaaac ggaaacgccg
      541 tctttattag gtaacatcca atccacgatc agcaaatcgt aattttcgac ttcggcctgt
      601 gtctgaccgg agcggccgtc atatacgata tccacggcat ttccggcctc actcaatccg
      661 cgctgaatga aggatgccac tttgcggtca tcttcgatca ataaaatgcg cataacaata
      721 ccttttcgat tggaaaaatt tacatctaaa acagacgtaa tgcaacgcat tgcgaattaa
      781 aaaacattaa tgcgcatgta ggatgatatg ctctgcgtcg ccccctcgta aagtagaagg
      841 tgttgaaacg aaaaaaggat tataccgtcg tccatataca ttaaaaaact taggactcat
      901 tcttaaaact atttaatgct tcattaatct ggttttaatg cgaggtatgt acattgggat
      961 caatcttatc aaaggagaag cgaattatga atccggcgca tatgcatctt ttattaaacc
     1021 acattccggt attggcttta gcggcggcga caatcttatt gatcgcagcg cgatggcgat
     1081 cgcaaataga atggacacga ttgagtatgc tcttttttgt aatcgcgggt gcggtatccg
     1141 tgccggtttt tatgaccggt gagccggcgg aaaaaatcat tgaaaacatc ggcggtatat
     1201 ccatgatgca tttggagtct cacgaagatt cagcggtagc gagtttgatc ggggcttgta
     1261 ttttgggggt actggccgta atagggcttt tttttatcga acgggtacac gctcatcgct
     1321 tgatatgggc gacctttttg atcattgcat tgacggccaa cggtttgatg gcatggacag
     1381 ccagtctcgg tggcaaaatt catcatacgg aaattcataa cggctacaca ccggcgcaaa
     1441 cacaaaaaaa gtatgatgat gattaaatta ttttttcagg agaactaaat tatgaaacga
     1501 tggattgttt acttgctgct ctgcgctggc ttttattttt tgcctgtgta cgggcaagaa
     1561 aataatgaaa aagacgatgc ccgtccttcg gtatccggtt acgcacagat catatattct
     1621 caatacctca aaaatcagga acatgatcgc cagagcgggt ttgatataaa tcgtgtgcgt
     1681 tttgcttttg aaggaagcgt cattcctgac cgggtacatt atgcggtgga gtttgatccg
     1741 caagggtcac cgatcctgga cgacacgtat cttgatttca ttatttctaa aaattcaaaa
     1801 gtccggttag gtcgctataa aattcctttc ggcgcagaga acccgaccgg tgaagacgaa
     1861 ctacatttca tcgaaaaaag taaggtggtc gaatccgtct tttccgcacg tgaagtaggc
     1921 atcggtacat attatgagcg aaattggatt atcggcgatg ttgggtactt tcaaaaatcg
     1981 gaagatgagc atatcgaacg cgacgtcatc gggcgaatcg gtcttaaacc gttcggaaaa
     2041 tatattgaaa ccggcggttc ggggtattac gaaaaaattc ataccgactc aaccaatttt
     2101 gccattcatc gtatcggcgc cgatctgacg tgtgaagtcg gaccgatcaa aatgcaaggc
     2161 gaatacatac gctccaaagg tacgatggaa gacaaacaac tttccggata cgggtattat
     2221 ggatacgggc aatacaccca tcatatcggt gatactcaaa tttcgtgggg cgtgcggtat
     2281 gaattttatc agccggataa aaacaacaaa tccgatcagg aaagaaagta tgtcgtaggt
     2341 tccacgatcg cattccatcc gagaatgcgg gcacaggtaa atttccaaca tatccgtgag
     2401 aatattgaaa ccaataatca gggacaaaat aaactgattg tccaatacca atttagtttc
     2461 tgatcagcga ggcattgcat gtttaaaaag tacagtgtac tcacagcggc ttgtattttc
     2521 ggtataggca cgttttcagt ttatcttttc ggggaatggc atgaaataca ggatgaaggt
     2581 ctgcgttggt ttttagaatt accgcacatg accttatctt tttttctgat catcatgctt
     2641 cctttagcgg tccatcagat ggctattctg attgagcgta agaatgagac gcggatgttg
     2701 tggacacgat ttatggtcgt cgtcgtgctg ccgtatatcg tcgtggcgac ctatctggcc
     2761 gccgaatggg atgaaatcga agaggaatgg ttgccgtggc tgatcaaacc ttctcatttt
     2821 ctgattgtat tgatcaatgg aattatgatt ccgggtatct tatggctgat cattaataaa
     2881 ttcattcagc tttgggatga aagggaacgt tttttaaaac gcatcgtcga gttagaaaat
     2941 gagttgcgtt catttgtgca tcccgaggtg tacaagcgta tttatgaaaa aacatcgctg
     3001 gaggctaccg gcgcatttaa aattatcatg gcggacatgc agggattcac caggttttgt
     3061 gaggcccatt cggctgcaga ggttgccaat ctggctaatg aattctgtaa cttgatggac
     3121 gaacgtattc ggcaggtcgg cggaatcaca ttatcctatg ccggtgattt agtgttcgcg
     3181 ctttttgatg aacatattgc aagtgacgat atcgtagggg tggcaaaaaa aatgcaggaa
     3241 acgtttcgtg cttttgcaca aactaaattt ccggattggg atacgaacct caatatcggt
     3301 attcattata gccgagatat cgtgctggga acgataggcg gaggaagtgc acagaaacat
     3361 tttacggcca atgggtttgg catttgcgaa gtgtcgcgta tgtgttcgga agccgatcat
     3421 ggccagattc tcgtttcgga agcatttgca gagaccgtca cgagtgaccc aatacgatca
     3481 cgtttgatcg gaattgcatg gagtaaacag cccaaaaata ttacggtgcc tccggttatg
     3541 tttgaatacc gtttggttga taatgaaata acttaaacgg taagggtgga actcaaagtt
     3601 ttttgatacc tgattacgcc gataccggag actcctgcat gatacgttcg atatcctgac
     3661 acacgcgtgt aataaggtcg ccgatttttt cgcgaacatt gggcgtaagc tcttcgccaa
     3721 attcaaaatt ggcgccctcg atcccgtaaa taatgacttc cggaacggga tggtgtagtg
     3781 ctttggccaa ttcgatagct tcggaaatac tataggcatg cgaagagtaa ttaaaaaaat
     3841 tgcccggtat aatatcggcg ttggcccgaa gcctgtagat ggtccccggt atgttatccg
     3901 aacacactgt atctatgatg ataacccatc gtgcatcaga ccatacatct tgcagcgaag
     3961 agccgtcgcc gttatgctcg atgacgcaca catccggtaa acacatgtct gctatggcgc
     4021 gcaccacttc gataccggcg cggtcatccc gccgatattc attgccgatg ccgatcacga
     4081 cgaccgacgc tgatgttgtg ataacattat attctttcat aagccgttcc tttaatggtg
     4141 aataaaacga acggtgcatt ttatacgtgt tcgatcatgt ccaaaatctg gagccgtgtg
     4201 gattccaggc gctgggtcac gatatgcgaa aaccgtttga gcaattggta tccgagttcg
     4261 tgatcagatt cgcatttgtt gcgcagacat cgtccgtcca gcgctatagc taatgtcaga
     4321 tccaaagccc gcgcatcaaa ttgccaatgg tacggcggga tgagccatga ccatccgagg
     4381 atatcgcctt cggtcaacgt atgtatgccg atgtgaccgc gaccgggcga tccgatttct
     4441 aatgcaatct tgccttggcg gatcagataa aattcgttag cctcctgccc ttcacgaaaa
     4501 ataatttgcc ccgtatcaaa ccgcacattg gaggcgcagc cgatgatcaa ttcaacatgt
     4561 tcggctgaca tatcaatcag taacgggtgc tgcatcagaa aaaacttcaa atctttcatg
     4621 gtggttttcc gtcttaaaaa taagcgccgg cataacaggc acacccggac atcaaacgat
     4681 acagccgtga gctgtatgta aacgaatcgt acacgtactc gggtcctgga tatcggggga
     4741 aggcgggaac gttatgccgg caaaagtttt catacaccgg actcctatag tcctgtaact
     4801 taagataagg ctaatgactc gacttcgcga cgacgtaggt catcgcgatg tgtgatatgc
     4861 gtcagcggtt taagtgaaat gactctaagt tgttgagatt tatagaagat caaagtacga
     4921 gaggggaggg ggtactcagg gttgaagttt aatacgttcc tttttaggga gttttttgac
     4981 gacttcgtcg taggaatgat cgatcatcag aaaaatttcc ttatcctgca cggatccgtc
     5041 caaatacacg ctattccaca attttttatt catgtggtac cccggttgaa cgccttcgta
     5101 acgctcgcgc cattcgatgg ctttttcggg atcggctttg agattgataa attcggcaat
     5161 atgagtattg atgagagcaa aaatttttcc gctgacccgg aataccaatg tttcctgatc
     5221 aaaaggaaaa tcttcatcgg ttgatttttt cttaagacaa tatgtgcgga tcgtttcgag
     5281 attcatgatg tgtgcgtaat tttagtttga attcacttcg ttaaaaaaac agaaccccgc
     5341 ttttcagcgg ggctcaacga tttccgggaa cccccgtagc acgtcgctag cgggcatcac
     5401 cggaggttcc cattaagtta tgcttaatac ggatcacctc ctatccccag ggttgaaact
     5461 tctcgcctga aatcgccctg aacaatttca cgcgaagcgt taattacgta ttaaacttac
     5521 aagctatcct ttccattgtc aatactcatt ttggcagata acactcctgt tgaaatgttg
     5581 ttctgaaaac atctgcaaat acggttttca aaaaacagtt cttgccaagg cgctaagttt
     5641 taaattacaa tcgaagcatt ttttatcttt gcgacttcgc gagggaattt tattgatggt
     5701 agcgtcggta agtacacgac gcctcggtac gttgcttcgt tcctactcgg tgtcagaagg
     5761 tgtattaata ctatagccgg atataagcgc ttgatggaga aatgatttgt gaagcggtgt
     5821 cctgcgtgag ggatgcggag ggtgagctcg cgtacgggcg cgagcgaaga ccccgtaaac
     5881 gcgccaaagg cgcgtgcgga ggccgatagc ccggagcaag cccgacccgc cacgccgtag
     5941 gcgtgacggg gcacgcccaa aaattaaata tctataattt attatgcacc ggcttcgtcg
     6001 cgtaaaccga aacgcatcgg atgcggcgga agattgatga tttcatccgg ataaaacgat
     6061 acgatataat caatcacgcc gcggacagat gtaaacgcaa ccggttcgcc gttttcattg
     6121 acgatcggga tatggcgtac atcgtgcagt gcaaggatac gaatcgccgc gcttacaggg
     6181 tcgtgcattt gcaaacactg cggcgcgaca cgcatatagt cgtccacttt agcagtatca
     6241 aaatccggcg tctcgtgtgc gatcttgtat aataaaaaac gctccgtcac ggtaccggtc
     6301 aattttttgt cacgtacgac catgacgcaa ccgatattgc gatcggccat ctgccgcacc
     6361 gcatctttga gcaggctgcc ggactccaca cacaaagccg cgggcggatc caggacaccg
     6421 atcggacggt caaaatcttc gggatcaatg ccttgctttt cgcgggcccg ttttttttcg
     6481 ggtgaacgcg gttttttttc ttcggtatca atccggacat gcattttggc caaaatgtga
     6541 tccaagatgt cttcgaacgt gagaatgccg ctcggatcgc cgacctggct gagtacggtg
     6601 atattgcgaa atccgccgaa cgtcatttca ttgagcgcgt actccgccga atcgtcggca
     6661 tacaaacaat gcgtatcggc gctcatcagg cgcgtaacgg gcgtatgtga aaattccata
     6721 tgcattgcga tcagattgcg aagaatggtc cgctcggaca gcgtcccgat caatgcgcct
     6781 ttgtcacgca ccaaaataca tccgcttttg gtttgctcca tcatcgcgac cacatcggcc
     6841 acggtcgcat ctttttctac atagagcggt ttgcggggat tgagtgaatc cacacgggct
     6901 ttggtaattc ctgcattcat ggtaacctcg ccgttaaaat gtccgttgaa cggaccgttg
     6961 atctatcgtt ttttccactt ctcgcatcca ttttttccga tcatgcgccc cacgtcatgc
     7021 attgaaaccg gtcggtatga tcggcgagaa ttttcttttt atcttcatcc gtactaaaaa
     7081 acaaacccgt agccagcgca tccgtgtcgc gtccattggt cccgatgata gtcaggcttt
     7141 ggaactgcgc ccgtttgggt tcggctaatt tgggatcgat caaatgatga tacacgcgtc
     7201 cgccggaacg gaaatactgt tcatacgtac ccgacgtcgc gatcgcctga tcgtgcagtg
     7261 tcacgactgt cgccatcaca tcggcacgtt cgggatcgcg cacaccgacg cgccagcctt
     7321 cgccgtcggc acgccggccc agtgcgacga tatctccgcc cagattgatc agcgcctgcg
     7381 tgatcccttc ttttttcaaa acttcggcgg cacgatcggc ggcataccct ttggcgatac
     7441 cgccaagatc aagccgtacg tcgggtgata aaatttttac cgtcgtgtcg gagcctgtcc
     7501 tcacatgaat aaaacgatgc agggaaagtc cggcgaaccg ctgccacacg tcttgcgcgg
     7561 gcggctccgt gcgatgtttc acatcccagg tatcgctcac ttttcccagt ccgggctcaa
     7621 atccgccgtc ggtcaccttg gaccaatgca gcgcccgttc gatcagatcg gctgtttccg
     7681 atgcgacgcg aatttccgcg cccgtcgtag cggcattgat acgtccgaca tcgctgaccg
     7741 gtgaaaaata tgtcaataaa ctttcaagtc tctgcaattc atcaaacgcg gcttccatgg
     7801 cgcgatcggc ccaacgcgca tcgtccgaca cgacatggat ttcgccgatc gttcccatca
     7861 gagggcgcgt catcgtgtac acacgacgtc ttgaaagaaa caacggtccc aaccctgctc
     7921 cggcacatac cagcagcatc gcgccgcttg ttttgagaaa ttgtcttcga tgcatatatc
//
LOCUS       DAOQNQ010000137         7937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3389_length_7937_cov_6.187262, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000137 DAOQNQ010000000
VERSION     DAOQNQ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7937
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3389_length_7937_cov_6.187262"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..808
                     /locus_tag="PK754_09380"
     CDS             74..808
                     /locus_tag="PK754_09380"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HNB09757.1"
                     /translation="MNIGKKIGICAHSYEGGALCFLSTCKEGAQILGAHYHPTIILSA
                     VPMGLSMPAYETNNHDEVGKYLREGVDRVAKAGADFFICPDNTAHIILEKIISTLPIP
                     GLHIADVVCKEIIDNGWKRVGLLGTNWTMTGPVYSQALKEKNLIKQIPDDSTREEIHK
                     AIFEELCQGIFRKETIAMFQNAIENLKNKGAECVILGCTEIPLIITPENSALPIVDST
                     RLLAKYAVRHALSENNSGDVGWIRII"
     gene            1358..3025
                     /locus_tag="PK754_09385"
     CDS             1358..3025
                     /locus_tag="PK754_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012930767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNB09758.1"
                     /translation="MKPTRRIVLFIFAITAVLLLGMVLQEKGKITLTKDMIAQAEKLF
                     GLQFSEAKRDSMLESLQEQLGNYEAMRKIKLDNSVAPALQFNPIPVGFKIDQDKKSFK
                     PARLDNVIPPPALEDLAYYSVSELAWLIKNKKVTSELLTKMFLDRLRRYGPKLENVIT
                     ITEGLAIKQARKADAEIAQGKYRGPLHGIPYGVKDLLATVGYKTTWGSVPYKDQLIDE
                     DATVVRRLEEAGAVLVAKLTMGELAWGDVWYDGKTRNPWDTTRGSSGSSAGSASAVSA
                     GLLPFAIGTETWGSIVSPSTECGTSGLRPTYGRVSRYGAMALSWSMDKIGPIARSAED
                     CALIFNAMYGPDGLDKTLVDAAFNYDASLKISNLRIGYLKSDMEKDSTRKAYYDTTLA
                     VLRKLGAKLTPIRLPQYPVYDMAIILSAEAGAAFDELTRSGKDDQLVRQIKNAWPNVF
                     RASRFIPAVEYIQANRIRTQLIQDMQNLMDTIDVYIAPPFEGDNLILTNLTGHPCVVV
                     PNGFTERNLPTSVCFMGKLFGEAEILALAKQYQDATDYHKRHPGLRF"
     gene            3219..5336
                     /gene="rnr"
                     /locus_tag="PK754_09390"
     CDS             3219..5336
                     /gene="rnr"
                     /locus_tag="PK754_09390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNB09759.1"
                     /translation="MLPEQKILQFLQSNAQSSFKSKDILRELHIRADQHDEAHGILQK
                     LVASGSIGKKGNKYFARAVSLTGTLSINKAGEGFVTVEGYEEDFFISPSRLKTAMNKD
                     TVMMIPYLRKRTGKRPEGEIIGIVERARTRIVGTVDSTGDFFVVIPDDRRLRRDIYVA
                     PEKTNGAKHGQKVVVSLEKWDDPSLNPEGSIIEIIGYPGDKGVDVLSVVKQHDIHFDF
                     PEAVEAECARIPATIPDDEIARRLDFRAALVFTIDPFDAKDFDDAISLDEGPQGHTIL
                     GVHIADVSYYVRENTALDREAYKRGTSTYLVDQVTPMLPEKLSNELCSLRPDEDKLTY
                     SCFMEIDAKGHVVDYDVVETVIHSKRRFTYEEVQQVIEGKPLDNLSPEVKKAVTDAYA
                     LSKRLTKKRMAEGSIDFDTPETKFKLDENGTPVECYRKERLDAHRLIEEFMLLANQYV
                     SKHIGLGSKKNELPFLYRVHDKPVKEKFENFVHLMKVLGHATHLPKSMDLLTPKHVQK
                     VIASVKGSKEDNLIEKVAIRSMAKAEYSDKNIGHFGLAFDYYSHFTSPIRRYPDLIVH
                     RMLKEYNAGMLRERIAWWSSKLPEMARHCSDRERVATEAERDSIKVKQAEFIERFLGE
                     EFDGIVSGVIQYGIFVEISQYLIEGLIHVRDLKGDYYVFDEKNYRLTGQRRKATFRLG
                     DRIRIKVARVNRDKREVDFTIVE"
     gene            5357..6454
                     /locus_tag="PK754_09395"
     CDS             5357..6454
                     /locus_tag="PK754_09395"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNB09760.1"
                     /translation="MRFPGINILDRYLIKKFLSVLFFALAGMVTIFVAVNYVENADRF
                     IDRKVPGNIIAEYYLNFIPHIITLTLPVDILLASLFSIGSLARFNELTAIKAGGRSLY
                     RVLFPIIVLALLVSAVNFYISEAIVPPANITKSEIWKSYVERASANRKVIGTDIHIYD
                     PSGTKVFIDKFDKRNRIISVMSIQQFYGTKLISRIDAGEATWDSTEAAWILRDVVERR
                     FTDSTETVIKHPTLAKRDLFFDPSDILRREQNPDEMNFIELRDFIHKLKRGGAQTERW
                     ETDYQLKFAYPLTCFFMVLFGAPLAASRKKSGAGINIFFTLVICLAYWSVIQTGRFMG
                     YNQSMDPVTAAWLGNSVFGVLSLLIFLRMKS"
     gene            complement(6462..6962)
                     /locus_tag="PK754_09400"
     CDS             complement(6462..6962)
                     /locus_tag="PK754_09400"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNB09761.1"
                     /translation="MRLVWMFLITAALPAYAQPDTTEQNLTRTRMAVVPFKNNTGSKQ
                     FEIITAGIAESITTALSGSGKFDFVERLQVDKALSEQDFQMTDLVDSKTVQSMGAILG
                     VEYIIIGDLQKIGTRFRINARRMRVSDGKLAESASASGEEDDLFDMQDEVAEKLIEQF
                     EQRNEK"
     gene            7308..>7937
                     /locus_tag="PK754_09405"
     CDS             7308..>7937
                     /locus_tag="PK754_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09762.1"
                     /translation="MRSALLKLLLMLSIGFTASAGTKTWNNASGGNWGTASNWLENAV
                     PGSGDTVYITLDGTYSVTYNLNFASVSAIYLGASSGIQTLINPGNANGVTGTMHINGN
                     GVFQMSSGTYSSSTVINYGTVRLIGGSFTFAPKIVNYDSVYISGTSIFVSGGLSNYDS
                     VFIAAGSFNLNGTDTSYAGKYLISFGATLNFNAGSHTLDANSSISGAGNV"
BASE COUNT         2184 a         1628 c         1846 g         2279 t
ORIGIN      
        1 cgtaacaatc taagcgatat gattttgctc aagtttgtaa tttattaatt tttttaaaaa
       61 ggattgaaca gtaatgaata ttggaaaaaa aatcgggatt tgcgctcata gctatgaagg
      121 cggagcttta tgctttcttt ctacctgtaa agaaggagcg cagatactgg gtgctcatta
      181 tcatcctacg atcatactta gcgccgttcc aatgggtttg agtatgccag cctatgagac
      241 caacaatcat gatgaagtgg gcaaatattt aagagaaggg gttgatcgtg ttgctaaagc
      301 gggtgcagat tttttcattt gtccagataa caccgcgcac attatcctag aaaagattat
      361 ttcgacgctt cctattccgg gattacatat tgccgatgtt gtctgtaaag aaattattga
      421 taatggttgg aaaagagtcg gacttttagg tacgaattgg actatgaccg gtcctgttta
      481 ttcacaagcc cttaaagaaa aaaatttaat caaacagatt cccgatgatt ctacaagaga
      541 agaaattcat aaagctatat ttgaagagtt gtgtcaggga atcttcagaa aagaaaccat
      601 tgcaatgttt cagaatgcca ttgagaactt aaaaaacaaa ggcgccgaat gcgtaatatt
      661 aggctgcacg gaaattccat taatcattac cccggaaaac tcggctcttc ctatcgttga
      721 ttcaactcga ctacttgcta aatatgctgt aaggcatgca ttatccgaaa acaactccgg
      781 agatgtcgga tggatcagaa ttatttgaga aatatttaaa tgtcgtttta tctgaagtag
      841 ttccgtaact ccggttcacg ttatcatcac gccgatcatc gtcaatagta ggccgtaatc
      901 tttagaaatg ccaaggatat tgtttcggtc acaattcttt gagaatagcg ataaccgata
      961 ggaaatgtac ctatgataga gtgtattaaa tagaatctcc agtttatttg aataattgac
     1021 cgagttgcgt ttgacttgag gcctgctttt ttttagccac atattattat gctatgctct
     1081 gatttacttc catatgtttt gccgaattaa ccctgccata ttgatgtata gtataattaa
     1141 ttttaaatat gatgacaaat cggggaactg agcaccacgg aaggaatttg atagttatat
     1201 ttctgttagt tttagcgcgg cgtataaagt gtaaaaaagc ttaccggctt tgtaaaaaca
     1261 atcattattg ccaataaatt attcgtttac aataagaggc tttgttttat ctacggtatt
     1321 cgtatatttt tctatccaaa tctaatgagg atttagtatg aaaccgaccc gccgtatcgt
     1381 tttatttatt ttcgcaataa ccgctgtgct tttgcttggt atggtattgc aggaaaaggg
     1441 taaaataacc ctaaccaaag atatgattgc tcaagccgag aaacttttcg gattgcagtt
     1501 cagcgaggcc aagcgtgatt cgatgctgga gtctctgcag gaacaactgg gtaactacga
     1561 agcgatgcga aaaatcaaac tggataattc cgttgcgccg gcgttgcagt tcaatcctat
     1621 ccccgtcgga tttaaaatag atcaggataa aaaatcattc aaaccggcgc gcctggacaa
     1681 tgtgataccg ccgccggcat tggaggatct cgcgtattat tcggtcagtg aattggcttg
     1741 gttaatcaaa aacaaaaagg ttacgtctga attattgacc aaaatgtttt tagatcgttt
     1801 gcgccgttac gggcccaaac ttgaaaatgt gattacgatc accgaaggat tagctatcaa
     1861 acaagcacgc aaagccgacg ccgaaattgc tcaaggcaaa tatcgcggcc cattgcacgg
     1921 cattccttac ggcgtcaaag atctcctggc gacggtaggt tataagacga cgtggggctc
     1981 tgtaccatat aaagatcaac tcattgacga agatgcgacg gttgttcgta gattagaaga
     2041 ggcgggtgcg gtacttgttg ctaaattaac tatgggcgaa ttggcatggg gtgatgtgtg
     2101 gtacgacggt aaaacgcgta atccttggga tacgacacgc ggatcaagcg gttcatcggc
     2161 cggctccgcg tcggcggttt cagccggatt gctgcctttt gcgatcggga ctgaaacctg
     2221 gggttcgatc gtatcccctt cgacggaatg cggaacgagc ggtctgcgac cgacatatgg
     2281 tcgtgtaagt cgttatggcg ctatggcgct tagttggtca atggataaaa tcgggcctat
     2341 cgcgcgttca gcggaagatt gtgcactgat atttaatgct atgtacggac cggacggttt
     2401 ggataaaact ttggtggatg cggcattcaa ttacgatgct tctttgaaaa tcagcaatct
     2461 gcgtatcggg tatctgaaat cggatatgga aaaagattct acgcgcaaag cgtattatga
     2521 tacgacgctg gctgtattga gaaaactggg tgcaaaattg accccgatcc ggttgccgca
     2581 atatccggtc tatgatatgg cgatcatctt atcggcggag gcgggggcgg cttttgacga
     2641 actcacgcgc agcggtaagg atgatcaact tgttcggcaa ataaaaaacg catggcccaa
     2701 tgtgtttcgc gcttcgcgct ttatcccggc ggttgaatat attcaagcca atcgcattcg
     2761 tactcagctt attcaagata tgcaaaacct gatggacacg atagatgtgt atatcgctcc
     2821 tccgtttgaa ggcgacaacc tgatacttac caatttgacc ggtcatccgt gtgtggtcgt
     2881 gcctaacgga tttacagaac gtaaccttcc aaccagtgtg tgttttatgg gaaaactttt
     2941 cggtgaagct gaaattttag cattagccaa acaataccaa gatgcaacgg attatcataa
     3001 gcggcatccg ggtttacgtt tctaaccata acttactttc aaaccaaaat ttttagagcc
     3061 gccgggtgtg atattaatac ccggcggttt tttattcaga tttcaataaa aacattagtg
     3121 taaaccttta ttccgtctta tattttcctg tttgatttga tatacttatc ctgtatggaa
     3181 tatttccatc gggtggttgt ttataaccgg aaaataactt gttgccagaa caaaaaattt
     3241 tacaattcct tcagagtaac gcgcagtctt ctttcaaatc caaagatata cttcgtgaac
     3301 tgcatatacg ggcggatcag cacgacgagg cccacggtat attgcagaaa ctcgttgctt
     3361 cggggtctat cggaaaaaag ggtaacaagt attttgcccg tgccgtgtcg ttgacgggaa
     3421 cactttcgat caataaagcc ggtgagggtt ttgtgacggt cgaaggatat gaagaagatt
     3481 ttttcatctc accgtctcgg ctcaaaaccg cgatgaataa agatacggtg atgatgattc
     3541 cctatctccg taaacgaacc ggtaagcggc ccgaaggcga aatcatcggc atcgtagagc
     3601 gtgcacggac gcgcatcgtg ggcacggtgg acagtaccgg agattttttt gtagtcatac
     3661 ctgacgatcg tcggttgcgt cgggatattt atgtggctcc tgaaaaaacc aatggcgcca
     3721 aacacggaca aaaagtggtg gtttccttag aaaaatggga cgatccttcg ctgaatcccg
     3781 aaggctccat tatagaaatc attggttatc ccggcgataa aggcgtcgat gtcttgtcgg
     3841 tggtcaaaca gcacgatatt cattttgatt ttccggaagc ggtcgaagcg gagtgtgcgc
     3901 gtatcccggc aactataccg gatgatgaaa tcgcacggcg gttggatttt cgtgcggcat
     3961 tggtttttac gatagatcct tttgatgcca aagattttga tgatgcgatt tccttagatg
     4021 aaggcccgca agggcacacc attctcggcg tacatatcgc ggatgtgagt tattatgtac
     4081 gtgaaaacac cgcacttgac cgggaagctt acaagcgtgg gacaagtacg tatctggttg
     4141 atcaggtgac gccgatgtta ccggaaaaac tttctaacga gttgtgcagc cttcggcccg
     4201 atgaggataa actcacgtac agttgtttta tggaaattga tgccaaaggg catgtcgtgg
     4261 attatgatgt cgtagaaacg gttattcaca gcaaacgacg gtttacgtat gaagaagtgc
     4321 aacaagtcat cgaaggtaaa ccgttggata atctttcacc ggaagtgaaa aaagcggtta
     4381 ccgatgcgta tgctttgtct aaacggctta ctaaaaaacg gatggcggaa ggcagtatag
     4441 attttgatac accggaaacg aaattcaagt tggatgaaaa cggcactccg gtggaatgct
     4501 accgcaaaga gcgtttggat gctcaccgtt tgatcgaaga gtttatgctt ttagccaatc
     4561 aatatgtcag caaacatatc ggcctcggca gcaaaaaaaa tgaattacct tttctgtatc
     4621 gcgtacacga taaacccgtc aaagaaaagt ttgagaattt tgtacatctg atgaaggtgc
     4681 tcggtcatgc gacacacctg cctaaatcca tggacttgct tacacccaaa cacgtgcaga
     4741 aagtgatcgc atcggtaaaa ggttccaaag aggacaatct gatcgaaaaa gtggcgattc
     4801 gctccatggc caaagcggaa tattccgata aaaatatcgg ccacttcggt ttagcgtttg
     4861 attattattc gcattttact tcacccatcc gtcgttatcc ggatttgatc gtgcatcgca
     4921 tgctcaaaga gtacaatgcg ggtatgttgc gtgaacggat tgcctggtgg tcatccaaac
     4981 tgccggagat ggcgcggcat tgttccgatc gggaacgcgt cgctacggaa gcggaacgcg
     5041 attccatcaa ggtgaaacaa gcggaattta ttgaacgctt tttgggcgaa gaatttgacg
     5101 gtatcgtcag cggtgtcatc caatacggga tttttgttga gatatcccaa tacctcattg
     5161 aggggcttat ccatgtgcgc gatctcaaag gcgattatta cgtgtttgac gaaaaaaatt
     5221 accgcctgac cggtcagcga cgaaaagcaa cgtttcgtct cggcgaccgg attcgcatca
     5281 aagttgcacg cgtcaaccgc gataaacgcg aagttgattt taccatcgtg gaataaaaat
     5341 tgatttcaat tcacatatga gatttcccgg catcaatatt cttgatcgtt atctgatcaa
     5401 aaaatttctt tcggtattat ttttcgcact tgccggaatg gtgacgatat ttgtagcggt
     5461 caattatgtc gaaaacgccg accgttttat tgaccgcaaa gtgcccggaa atataatcgc
     5521 cgaatattac ctcaacttta ttccgcacat catcacgctc accttgccgg tggatatttt
     5581 gctggccagt cttttttcca taggttcgct ggcgcgtttc aacgaattga cggcgatcaa
     5641 agccggcggc cgaagtttgt atcgcgtact gtttccgatc atcgtactcg cactgctggt
     5701 cagtgcagtt aatttttata tcagtgaggc gatagttcct ccggccaata ttaccaaatc
     5761 ggaaatatgg aaatcctacg tcgaacgtgc ttcagccaat cgaaaagtga tcggtacgga
     5821 tatccacatc tatgatccga gcggtacgaa ggtgtttatt gataaatttg acaaacgaaa
     5881 tcgcatcatt tccgtcatgt ccatccagca gttttacggt accaaactta tatcgcgcat
     5941 tgatgccggt gaagcgacat gggatagcac ggaagcggcg tggatactgc gtgatgtcgt
     6001 cgaaagacgt tttaccgata gtacggaaac cgtgatcaaa catcccacgc tggccaaacg
     6061 cgatcttttt ttcgatccga gtgatatcct gcgcagagag caaaatccgg acgagatgaa
     6121 ttttatcgaa ctgagagatt ttatccataa actcaaacgc ggtggtgcac agacggaacg
     6181 ctgggagaca gactatcagc ttaagtttgc gtaccctctt acctgctttt ttatggtact
     6241 ttttggcgct ccgctggcag cctcacgcaa aaaaagcggc gcagggataa atatcttttt
     6301 tacgttggtt atctgtctgg cttactggag tgtaatacaa accggtcgtt tcatgggata
     6361 taatcaatcc atggatccgg tcacggcggc gtggctgggt aattcggtgt tcggcgttct
     6421 atcgttgttg atttttttgc gaatgaaaag ctgaatagac gttatttttc attacgctgt
     6481 tcaaactgtt cgatcagctt ttctgcgact tcatcctgca tatcaaacaa atcatcctct
     6541 tcaccggaag cgctggccga ttccgccagt ttgccgtcgc ttacacgcat gcggcgcgca
     6601 ttgatacgaa atcgagtgcc gattttttgt aagtcgccga taatgatgta ttccacgccg
     6661 agaatagccc ccatagactg cacggttttg gaatccacga gatctgtcat ctgaaaatcc
     6721 tgttccgaca gggctttatc tacctgcagg cgttcgacaa aatcaaattt tcctgagccc
     6781 gatagtgcgg tggttatgct ttcagcaata cctgcggtaa tgatttcaaa ttgttttgaa
     6841 ccggtattgt ttttaaatgg tactacggcc atacgtgttc tagttaggtt ttgttctgtc
     6901 gtatcgggtt gggcgtatgc cggtaaagca gccgtgatca aaaacatcca tactaaacgc
     6961 atgggttatt ctcctgaaat gtggtatgtg tttatttacg acagaaccat atcgtaaacg
     7021 ctgcaaatat taagtttttt tgttttaaaa agcacgaaaa tcggactata catagaaata
     7081 cgtatttcgg ctgagcatgt cattttcttt gccttggtat aaactataag ttaaatttta
     7141 tcggttcttg cctcatcagt tctattcctt agtttctggc ttgtatatcc catctttatg
     7201 ggatggattt cacgttcaga taaagcgact ttgtacctat ctcataaggt tcgtttaata
     7261 tattttttat tacataatac atcgtcttta tcataggagg aagatatatg agatcagcct
     7321 tactgaagct gctactcatg ctttccattg gatttaccgc atcggcgggc acaaaaactt
     7381 ggaataatgc atccggcgga aattggggca ccgcatccaa ctggttggaa aacgccgttc
     7441 ccggttccgg tgatacggtt tacatcacac ttgatggaac ttattccgtt acatacaatc
     7501 ttaattttgc atcggtgtct gcgatttatt taggtgcatc gtcaggtatt caaacactga
     7561 ttaatcccgg aaacgctaac ggagtcaccg gaacgatgca tatcaatggt aacggcgtgt
     7621 ttcaaatgtc gtcgggtact tattcgtcat ccacggtgat caattacggt actgtacgat
     7681 tgatcggagg gtcgtttacc tttgctccaa agattgtcaa ctatgacagt gtttatattt
     7741 caggtacaag tatctttgta agtggtggtt taagcaatta tgacagtgtg tttattgcag
     7801 caggtagttt caacttgaat ggaaccgaca cgtcgtacgc aggaaaatat ctgatttcct
     7861 tcggtgcgac attaaatttc aatgcaggct cgcatactct agatgctaat tcatccatca
     7921 gcggtgcggg aaatgta
//
LOCUS       DAOQNQ010000138         7882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3429_length_7882_cov_6.222052, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000138 DAOQNQ010000000
VERSION     DAOQNQ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7882
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3429_length_7882_cov_6.222052"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PK754_09410"
     CDS             complement(<1..433)
                     /locus_tag="PK754_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09763.1"
                     /translation="MPFPRLTKLQVKEYSEVLDKRTRKQKALFHIEGVRLFEEFSSSG
                     WEAEWIAVDTPFLEAHPQAAEFLKKKFGVCTWLASSLDIKKLSDTEHAQGIVAAIRKP
                     AAIDENGRAPLGNVLILDQIADPGNLGTMLRSADWFGVDHVY"
     gene            complement(468..881)
                     /locus_tag="PK754_09415"
     CDS             complement(468..881)
                     /locus_tag="PK754_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNB09764.1"
                     /translation="MKPIKVLVAKPGLDGHDRGAKIIASALRDAGMEVIYTGLRQTPE
                     MIVQAALQEDVDVIALSILSGSHMTLFPKIMTQIKEAELDDVLVIGGGIIPDEDAAAL
                     EKTGVQKIFGPGTSTSDVVDFIRAWYEKSSRRVLV"
     gene            complement(889..1629)
                     /locus_tag="PK754_09420"
     CDS             complement(889..1629)
                     /locus_tag="PK754_09420"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNB09765.1"
                     /translation="MSSNTKLSTKPRDGKKGARIVFASETLDLGVIREGDIVDGVFSF
                     SNAGDDTLKIQNVKASCGCTAALLSSDIIPPNGKGEIKATFNSRGKVGNIHKTITVFS
                     NSADNPVTQLNLVVKVEGNGSAHTGGMKKSNAMHPEVEGDMHGSYFEGKCANCHVEPG
                     VGKFGKDLFDVSCAMCHGADGRGAVAQPLRGGEFLNRADASLLQERIAKGSPSNVMMR
                     GFAKSAGGPLDETQIKSLVEYIGSWKHK"
     gene            2031..2789
                     /gene="recO"
                     /locus_tag="PK754_09425"
     CDS             2031..2789
                     /gene="recO"
                     /locus_tag="PK754_09425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNB09766.1"
                     /translation="MAIITTEAVILRSTPYRETSKIVTALTKDHGKLSLIARGARDTK
                     SKYGGALELFNHISVIYYERANRDMFYISDASVIRGFTHIQKDLSRTYIAMAILEMAN
                     RVVHGNEEHQGFFELILQTLAGLHEEQRPKNGLLFFLMAMADNLGFTMDFENCDFCDN
                     RVKNKDILRFNSDRGRLVCGECPREFHVHDTPALSKESIAVMAHLARCRGRGVYNLTI
                     SERSTQEVFQVLLQHLQRHIDELRTLQTLAYLRL"
     gene            2819..3403
                     /gene="lptC"
                     /locus_tag="PK754_09430"
     CDS             2819..3403
                     /gene="lptC"
                     /locus_tag="PK754_09430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNB09767.1"
                     /translation="MLSCTALTEKEDLPAGEKPDRPGQEGWNSVAVFSSGGVKDAELH
                     FAHMQKWDTKQLTTFRNGLRVEFFEKGTHSATLTSDSGEIRSNTNNLFAYGNVVIVSD
                     SGVSMQTEKIYWDHQKQRIFADGLVTMTTDEDTLRGYQFESNKDLTNWKMKNASGRSA
                     RDIDLRTGTVKPKTTRRNDTELDKEMDAMIKERP"
     gene            3400..4590
                     /locus_tag="PK754_09435"
     CDS             3400..4590
                     /locus_tag="PK754_09435"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HNB09768.1"
                     /translation="MRSFCYFIITLCFFAVVHSLWAQQNEDYKPLKLDEADEIENQRI
                     DGKDVMKARGDVRFSHDTLTATCDQAAFFREMQSAILIGNVVLNDRHRTIYCEKARYY
                     ARQKRAVCEGNVIFVDRATTLVADSLVYFTQSEQLTAQGQVVIYDSTEEMTMYGERGF
                     YDAKRRYANATGHPYMIQFDSTHYKGQNIARLSRGIYTQPPPRDSVTGKPVRFSPDEQ
                     ITARGLFVEVYQDSHLVYVRDSVEFLREKLRTTSGKARYHTKKEFLTLQKEPIADYAL
                     NRMVGDSMIVQFRKRAIETMYVIGNAIATSSVDTLGTMVNTLQAKQITMHVKDKHLDV
                     MEAQGNAYNVYYLEGREGANRISGPNMSLFFDTKGKLRNFRVDGGAEGTYFPENLKNR
                     IEKP"
     gene            4587..5390
                     /gene="lptB"
                     /locus_tag="PK754_09440"
     CDS             4587..5390
                     /gene="lptB"
                     /locus_tag="PK754_09440"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNB09769.1"
                     /translation="MSETVQHEQPSLPALADYIDHQSDQKKTLRAEGLVKRYGSRTVV
                     NGVNVQVTQGEIVGLLGPNGAGKTTTFYMITGMIKPNHGSVFLDEKKITTIAMYRRAQ
                     LGVGYLAQEASIFRKLSVEDNIMAILETLPLKRKARRERLDQLLEDMRITHIRKSKGY
                     ALSGGERRRTEIARALVTNPRFLLLDEPFAGIDPIAVEDIQSIVQGLKSRGIGILITD
                     HNVHETLAITDRAYLLFDGRVLKSGTAQFLADDPEARKLYLGDKFKLRD"
     gene            5412..7070
                     /gene="rpoN"
                     /locus_tag="PK754_09445"
     CDS             5412..7070
                     /gene="rpoN"
                     /locus_tag="PK754_09445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNB09770.1"
                     /translation="MKQILSQQMKLSPQQVMLSSLLQLPITALEQRLKQELEQNPLLE
                     ESQELEELQDEEMELEQTQEKPELKQEQEKPEDVAKEEEVAIQAEKDKEKKEEEEVDW
                     AKILNDDSSFEIRMPRGQGEDDDDQEWIQPDKVSLSDHLLEQLRWTSLTEEQRRIAEY
                     IIWNLNEDGYFVMDDPLTGETPIADEVQRFTEAVGEFRDDTLLADKPDEPAEESERKT
                     THRKKTTPKIDAVQFVADEFQTTAEAVEKILKTIHTFDPPGIGARHLKECILIQLYRR
                     KESDGFYQNGTDITIRIIHEAYEDFINRRYDKVAKMLNIPIDAIKHSISEVLSINPKP
                     GEGYITPEQNYITPDAAVKKIDGKFEIILNDYDVPHLRINNAYKRMMLERNKTGKETK
                     DFIKNKLEAAKWLINSLYRRRDTIYRTIESIVELQQEFFEKGVHHIKPMKLEDVAVRI
                     GMDISTISRATNGKYVQTDYGVFELKYFFSTAMTNSDGEDVSTKQIKTLLKEVIDQED
                     KRHPYSDEELAKVLTERGTPIARRTITKYREQMMIPSGRLRKQI"
     gene            7106..>7882
                     /locus_tag="PK754_09450"
     CDS             7106..>7882
                     /locus_tag="PK754_09450"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HNB09771.1"
                     /translation="MNYRIVMCAGLLLAGLASEGRASMNKPSIEYTLRMPAPHTHYFE
                     VTMTMQGIKKDYIDIIMPVWTPGSYKVREFSRNVENVRATSGSQSLRVEKISKNTWRV
                     FSGKTENVAVNYAVYAYELSVRTSYMDASHGYVNGASVFMYADGYQSLTHTVTIELYN
                     EWKHISTGLKPVSGSTHRFVADHYDVLVDSPIEMGNQKILNFELQGVPHEIAIYGIGN
                     YDTEAMVRDMRKIAETTAAMFGKLPFERYVFIIHCVPGYGG"
BASE COUNT         2299 a         1926 c         1834 g         1823 t
ORIGIN      
        1 aatacacatg gtccacgcca aaccaatccg cactgcgcaa catcgttccc agattaccgg
       61 gatctgcgat ctgatcaagg ataagaacat tacccaaagg cgctctgccg ttttcgtcta
      121 tcgcagcggg ttttcttata gcggcgacga taccttgggc atgctccgta tccgaaagtt
      181 ttttaatatc cagactggaa gccaaccatg tgcaaacgcc aaatttcttc ttcagaaatt
      241 ccgcagcctg cggatgagct tccaaaaagg gtgtgtctac ggcgatccat tcggcttccc
      301 atccggagga tgaaaattcc tcaaataacc tgacaccttc gatgtgaaat aatgcctttt
      361 gtttccgggt gcgtttgtcg agcacttcgg aatattcttt gacttgcagt ttggttagac
      421 gcggaaaagg catcgtttaa tttttaagaa ccgtaaaaag aaaagactca gaccaaaacg
      481 cggcgcgagc ttttttcgta ccacgcacgg ataaaatcaa cgacatccga tgtggatgtg
      541 ccgggaccga atattttttg aactcctgtt ttctccagcg cggcggcatc ttcatccggt
      601 atgatacctc cgccgataac taacacatca tcaagttcgg cctctttgat ttgtgtcatt
      661 attttaggaa agagggtcat gtgcgaacct gaaagaatgc tcaacgcaat aacgtccacg
      721 tcttcctgca acgccgcctg cacgatcatc tccggcgttt gacgcagacc ggtatatatg
      781 acttccatgc cggcatcccg caatgcgctg gcaatgattt tggcgccacg gtcatgaccg
      841 tctaaaccgg gtttggccac taaaactttg atcggtttca tacaaaactc acttatgttt
      901 ccaacttccg atatattcta caagcgattt tatctgtgtt tcatccagcg gacctccggc
      961 gcttttggca aaaccgcgca tcatgacatt ggacggcgat cctttggcga tacgctcctg
     1021 cagcagcgac gcatccgcac gattgagaaa ttcaccgcca cgcaaaggct gcgctacggc
     1081 gccgcgaccg tctgcaccgt gacacatcgc acacgacacg tcaaataaat ctttaccaaa
     1141 ctttccgacg ccgggttcga catggcaatt agcacatttg ccttcgaaat acgaaccgtg
     1201 catatcgcct tcgacttccg gatgcatggc gttggatttt ttcatcccgc cggtatgagc
     1261 ggagccgtta ccttcgacct tgacgacaag attgagctgg gtgacgggat tatcggcgct
     1321 attggaaaaa acagtaatcg ttttgtgaat gtttccgact ttgccgcgtg aattgaaggt
     1381 tgctttgatt tcgcccttac cgttcggcgg tatgatatcg gaactgagta aagccgccgt
     1441 acaaccacag gatgctttga cgttttgaat tttgagtgtg tcatcgccgg cattggaaaa
     1501 agaaaaaaca ccatctacga tatcgccttc ccgtattacg ccaaggtcta atgtttccga
     1561 tgcaaaaaca atacgtgctc cctttttacc atcccgaggt ttagtggata attttgtatt
     1621 ggatgacaaa aaaaatccta aggcgaacaa aacacccgta aaaaaaagta atgaaatttt
     1681 ttctcgcata attttaaatc aatttaaagt tcccatgaag agattttggt tacccgtaaa
     1741 aatacgtatt cactaaaata catgcaagtc ttaatgttta caaaagttta cgtagatata
     1801 aaaacaaacc caagcgtcaa atattcagtt aatgacacca cacttaaaaa taaaagctaa
     1861 ttgccaattc ataaaacgaa taaaaactta actacttaac tcaattctaa aaagtggcaa
     1921 attttgagcc aatactacat atcatcgttg cttttagctc gtgaatgaaa tatcttaatt
     1981 tcagcaaaaa gcgagccaaa agaattcaca ttgtagtatg ttgttaaaac atggcgataa
     2041 taaccacaga agcggtgata ttgcggtcaa cgccgtatcg cgaaacgagc aagatcgtca
     2101 cagcgctgac caaagatcac ggcaaactta gtcttatcgc acgcggagcg cgcgacacca
     2161 aaagcaaata cggcggagcg ctggagcttt ttaatcatat ctcggtcatt tattacgaac
     2221 gcgccaatcg ggatatgttt tatatatccg acgcatcggt cattcgcggg tttactcata
     2281 ttcaaaaaga cctctcccgc acctatatcg cgatggcaat tttggagatg gctaaccgcg
     2341 tcgtgcacgg caacgaagag catcaaggat tttttgaatt gatcctgcaa acgctcgccg
     2401 ggctgcatga agagcagcgt cctaaaaacg ggttgctttt ttttctgatg gcgatggccg
     2461 ataatttggg atttacgatg gactttgaaa actgtgattt ttgcgataac cgtgttaaga
     2521 ataaagacat cctgcgtttc aattccgatc gcggaagatt agtttgcggc gaatgcccgc
     2581 gtgaatttca cgtacacgat acaccggcat tatccaaaga aagtatcgcc gtgatggcgc
     2641 atctggcgcg gtgccgaggt cgcggcgtgt ataatctgac gatcagcgaa cgcagtactc
     2701 aggaagtatt tcaggtatta cttcagcact tgcaacgtca tattgacgaa ttgcggacgc
     2761 ttcaaacttt ggcgtattta cgattatgaa atattggttc attataacgt acacaacttt
     2821 gttgagttgt acagcgctga ctgaaaaaga agacctgccg gccggcgaaa aaccggaccg
     2881 ccccgggcag gaaggttgga actcggtggc cgtgtttagc tcgggcggcg tgaaagacgc
     2941 agaattacat tttgcacaca tgcagaaatg ggataccaaa caattgacga cttttcgaaa
     3001 cggactgcgc gtggagtttt ttgaaaaagg cacgcatagc gcgacactga cttcggacag
     3061 cggcgaaatt cgctccaata ccaataatct tttcgcctat ggcaatgtcg tgatcgtttc
     3121 cgacagcggc gtatccatgc aaaccgaaaa aatttattgg gatcatcaga aacaacgcat
     3181 tttcgccgac ggacttgtca ccatgacgac cgacgaggat acgttacgcg ggtaccagtt
     3241 cgaatccaat aaagatttga ccaactggaa aatgaaaaat gcaagcggtc gttcggcacg
     3301 ggatattgat ctgcgtacag gtaccgtcaa gcccaagact acacgccgta acgatacgga
     3361 acttgataaa gagatggatg ccatgatcaa ggagcgtccg tgagatcgtt ttgctacttc
     3421 atcataacct tatgcttctt cgccgtggtg cattctcttt gggcacagca aaatgaagat
     3481 tacaagccgc tcaaactgga tgaggccgac gaaatcgaaa accagcgcat tgacggcaaa
     3541 gatgtgatga aggcacgcgg cgacgtacgt ttttcgcatg acacattgac ggcgacgtgc
     3601 gatcaggctg ctttttttcg cgaaatgcag tcggcgatcc tcatcggcaa cgtggttctc
     3661 aatgaccgcc accgtacgat ttactgcgaa aaagcgcgtt attacgcccg gcaaaaaaga
     3721 gccgtatgcg aaggcaatgt gattttcgta gatcgcgcaa cgacactggt ggccgattcg
     3781 ctcgtgtact ttacccaaag cgaacaatta acggcgcaag gtcaggttgt gatttacgac
     3841 agcacagaag aaatgaccat gtatggcgaa cgcggatttt atgatgctaa acgtagatat
     3901 gcgaacgcga ccggacatcc ttatatgatc caatttgaca gtacccatta taaaggtcaa
     3961 aatatcgccc gcctgagtcg cggcatatat acccagccgc cgccgcgtga cagtgtaacc
     4021 ggaaaacccg tgcgtttttc gccggatgaa caaatcacgg cgcgcgggct tttcgtcgaa
     4081 gtgtatcagg acagccatct cgtctacgtg cgcgacagcg tggaattttt acgcgaaaaa
     4141 cttcgaacca catccggcaa agcgcgttat cacaccaaaa aagaatttct gacgctgcag
     4201 aaagaaccga ttgccgatta tgcactcaac cgtatggtcg gtgattcgat gatcgttcag
     4261 tttcgcaaac gcgcgatcga aaccatgtat gtcatcggaa atgccatagc cacttcgtcg
     4321 gtggatacgc tgggcacgat ggtcaatacg ctccaagcca aacaaatcac catgcatgtc
     4381 aaagataaac acctggatgt gatggaagcg cagggaaatg cgtataatgt atattatctc
     4441 gaaggccggg agggcgccaa tcgcatatcc ggtcctaaca tgagtttgtt ttttgatacc
     4501 aaaggaaaat tacgtaattt tcgcgtggat ggcggagccg aaggcaccta ttttcccgaa
     4561 aacctgaaga atcgaatcga aaaaccatga gcgaaaccgt acaacacgaa caacccagtt
     4621 taccggcatt ggccgactat attgatcacc aatcggatca gaaaaaaaca ttgcgtgccg
     4681 agggattggt caaacgctac ggcagccgca ccgtcgtcaa tggcgtgaac gtacaggtca
     4741 cgcagggtga aatcgtcgga cttctcggtc ctaatggcgc cggaaaaacc actacgtttt
     4801 acatgattac gggtatgatc aagcctaatc acggctcggt ctttcttgat gaaaaaaaga
     4861 taaccacgat cgccatgtac cgccgtgcac aattaggcgt cggctacctt gcgcaggaag
     4921 cttccatttt ccgaaagctc tccgtcgaag ataatatcat ggccatcctc gagacgctgc
     4981 ccttgaaacg caaagcccgg cgcgagcggc ttgatcaact gctggaagac atgcgcatca
     5041 cgcacattcg caagagtaaa ggttatgcac tttccggcgg cgaacggcga cgcaccgaaa
     5101 tcgcacgcgc cctggtcacc aatccgcgct ttcttcttct cgacgaacct tttgccggca
     5161 ttgatccgat cgccgtcgag gatatccagt ccatcgtgca agggttgaaa tcacgcggca
     5221 tcggtattct catcacggat cacaacgtgc acgaaacctt ggccatcacc gaccgcgcat
     5281 acctgctatt tgacggacgc gtactcaaaa gcggaacggc acaatttctg gccgacgacc
     5341 ccgaagcgcg taaattgtat ctcggcgata aattcaaatt gagggattag atcaggatta
     5401 tggcacaggg gatgaaacaa atactcagcc agcaaatgaa gctgtcaccg caacaggtga
     5461 tgctatccag cttgctgcag ttacctatca ccgcgttgga acaacggctc aaacaggagt
     5521 tagagcaaaa cccgctgctc gaagaatcgc aggaactcga agaactgcaa gacgaggaaa
     5581 tggaactcga acagacgcaa gaaaaacctg aactgaaaca ggagcaagaa aaacccgaag
     5641 acgtggccaa agaggaagaa gttgctatcc aggcagaaaa agacaaagag aaaaaagagg
     5701 aagaagaagt cgattgggcc aaaatcctca acgacgacag ttcgtttgag atccgaatgc
     5761 cccgcggcca gggcgaagac gacgatgatc aggaatggat ccaaccggac aaagtatcgc
     5821 tcagcgatca tttactcgaa caactgcggt ggacttcgct gactgaagaa caacgccgta
     5881 tcgcggaata tatcatctgg aatctgaatg aagacggata ttttgtgatg gatgatccgc
     5941 tcaccggcga aacaccgatc gccgatgaag tgcagcgctt taccgaagcc gttggtgaat
     6001 ttcgcgacga tacgttgctg gccgataaac cggacgaacc cgcggaagaa tctgaacgca
     6061 aaacgacaca tagaaaaaaa acaacaccta aaattgatgc ggtacaattc gtcgcggatg
     6121 aatttcaaac cacggccgaa gccgtcgaaa aaatattaaa aaccatccat acgtttgatc
     6181 cgcctggcat cggtgcgcgc catctcaaag agtgcattct gatccagctg tatcggcgta
     6241 aagaatcgga tggattttat caaaacggca cggatatcac tatacgcatc atacatgaag
     6301 cctatgaaga ttttatcaat cgccggtacg ataaagtcgc taaaatgcta aatattccca
     6361 tagacgccat caaacacagc atcagcgaag tactttcgat caatcccaaa cccggcgaag
     6421 gatatattac gccggaacaa aactatatca cgcccgatgc cgccgtcaaa aaaatcgacg
     6481 gcaaatttga aatcatactc aacgattacg atgtgcccca tctgcgtatc aataatgctt
     6541 acaaacggat gatgctggag cgcaataaaa cgggcaaaga aaccaaagac ttcatcaaga
     6601 ataaactcga agccgcaaag tggcttatca attcgctcta ccgtcgccgg gatacgatct
     6661 atcgtacgat cgagtccatc gtcgaactgc agcaggagtt ttttgaaaaa ggcgtccacc
     6721 acatcaaacc gatgaaactt gaagatgtgg ccgtacgtat cggtatggat atttcgacga
     6781 tcagccgagc gaccaacggt aaatacgtcc agaccgatta cggcgtattc gaattaaaat
     6841 atttcttctc cacggccatg accaacagcg acggtgaaga cgtctccacc aaacagatca
     6901 aaactttatt gaaagaggtt attgatcagg aggacaaacg ccatccttac agcgatgaag
     6961 aattggctaa ggtccttacc gaacgcggta cgccgatagc acggcgcacg atcaccaagt
     7021 accgcgaaca aatgatgatc ccctcggggc gtctccgcaa acaaatctga aatgaaccta
     7081 actattttca tgaaggaacg acggcatgaa ttatcggatc gttatgtgcg caggcctgct
     7141 cctggccggg cttgcgagcg aaggacgtgc aagcatgaat aaaccctcca tcgaatacac
     7201 cttgcgcatg cccgcgccgc atacgcacta tttcgaagtg acgatgacga tgcaaggcat
     7261 aaaaaaagat tacattgaca ttattatgcc cgtatggacg cccggttcat acaaagtgcg
     7321 tgagttttca aggaacgtgg aaaatgtgcg cgccacgtcg ggttcgcaat ctctgcgcgt
     7381 tgaaaaaatt tccaaaaaca catggcgcgt gttttccggt aaaacggaaa atgtcgccgt
     7441 aaattatgct gtatatgcct acgaactcag cgtccgcaca agctatatgg atgcctcgca
     7501 cggttatgtc aatggcgcca gtgtttttat gtatgccgac ggatatcaaa gtctgaccca
     7561 taccgtgacg atagaacttt acaacgagtg gaagcatatt tcgaccggcc tcaaaccggt
     7621 atccggcagt actcaccgat ttgtcgcgga tcattatgat gtccttgtgg attcccccat
     7681 cgaaatgggt aaccaaaaaa ttttaaattt tgaattgcaa ggcgtgccgc atgaaattgc
     7741 catctacggc atcggtaact acgataccga agcgatggtt cgagacatgc gaaaaattgc
     7801 tgaaacaacc gcagccatgt tcggtaaact gcctttcgaa cggtatgtgt tcatcattca
     7861 ctgcgtaccc ggttatggcg gc
//
LOCUS       DAOQNQ010000139         7877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3434_length_7877_cov_11.780747, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000139 DAOQNQ010000000
VERSION     DAOQNQ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7877
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3434_length_7877_cov_11.780747"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..2412
                     /locus_tag="PK754_09455"
     CDS             178..2412
                     /locus_tag="PK754_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09772.1"
                     /translation="MKRRLWIHTIIALSTLILTVVLHQRIGRAGIDAKTSLRLRLKKP
                     DIIDARFIAGGNWYYVMRNQGAFMYDRGDWDNNGNNAGGAFPRGSGATVVYAAGPWIG
                     AIKNGVPVVSETQFASEFQPGRILNSGVPFEELIADSVDNPLNQVYVVDRNTTPNEWT
                     QLPSEFKNQYGNSPSLIADVQAWTVFNDLDTTLSQETSHSSPFPGLGLEITLESMMYE
                     TPFLNNSVLLHYTITNKTSTPYTGAYFGLWMDADVDAASNDLVGSDSTRGLGYIYNAD
                     NTDKPLAVGFDILQGPVVHKDSIPAQYAYKFQNNTSVLQLNPITKKYESKTISSDSLV
                     IGAITANSYFGAADPFNNFDRYTLLQGIEQYGYPYFINGWYFPGNPVTGTGSIHMVGS
                     SQRILVATGPFRMEAGGSQEIWAAVVGGEGVDRLDAVANLFQNDDVLQSAFTSGIHSL
                     VLPSPSLSVTAGDGFVTLTWDSLAEEASDPIAALDMINTDNGYSRDLLLHDFQGYRLW
                     KSLTALPGQFDLIAQFDLNDGIRSVRRWYLSPSGKLSLSNVSVGEDTGIEYTYTDHDV
                     RNGRRYYYALTAYDAQPAVYGPDSVYYADLGRLIPKPLIMNPSTESSLWDNITAVTPQ
                     PKKAESKKELSHILVVPNPFYATTSEGEQIKFTGLPASCTIRIFTVAGDLVKTIKHHA
                     TSNNNRVDDTLLNEDTSPLAQETSVEVWNLKNEKKHPLAPGIYIAHIHSSLGDKIVKF
                     AVIR"
     gene            complement(2640..3056)
                     /gene="atpC"
                     /locus_tag="PK754_09460"
     CDS             complement(2640..3056)
                     /gene="atpC"
                     /locus_tag="PK754_09460"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNB09773.1"
                     /translation="MDNNKYHLEIISPRHVVFKGEVESFTAPGASGSFQILKNHTAYI
                     SSVLIGEVKIRHTDGYDEIYATSGGFVEVEHNKVTFLAETCEKVSSIDLIRAQESYER
                     ARKRLEDRSSYDPERSRQALLRSRNRLRLAEKYNKQ"
     gene            complement(3078..4475)
                     /gene="atpD"
                     /locus_tag="PK754_09465"
     CDS             complement(3078..4475)
                     /gene="atpD"
                     /locus_tag="PK754_09465"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNB09774.1"
                     /translation="MNKGKIVQIIGPVVDIEFANGQLPEIYNSAHVTRPDGSTLVVEV
                     QQHLGENRVRAVAMDATEGLVRGMDAVDTGAPIAVPVGEKVLGRLLNITGDTIDGFGK
                     IEASNKRMPIHRPAPKLEELTTKSEMFETGIKVIDLIEPYMRGGKTGLFGGAGVGKTV
                     LIQELIHNIAKQHGGYSVFAGVGERTREGNDLYHEMKDSGVLNKTALVFGQMNEPPGA
                     RQRVALTALTMAEFFRDDEGRDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPNLA
                     TEMGELQERITSTKKGSITSVQAVYVPADDLTDPAPATTFAHLDATTVLSRAISELGI
                     YPAVDPLDSTSRILDPLVVGQEHYDTAKRVQEILQKYKDLQDIIAILGMDELSDDDKI
                     VVARARKIQRFLSQPFHVAEQFTGTPGRYVKKEDTIAGFKAIAEGQMDDLPEQAFYMV
                     GTIDEAEEKAEKMRR"
     gene            complement(4508..5599)
                     /locus_tag="PK754_09470"
     CDS             complement(4508..5599)
                     /locus_tag="PK754_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09775.1"
                     /translation="MTIRYVFLVATMVTRLTAGEENAVGFTFLGIRPDARSAAIGPMV
                     AIPNAWSAFYNPAGIAFASGLGMSYNTNDYVANTSINYWTAGMGHTRFGSLAVNNYRF
                     NYPRIERTNEENPDGTGEYYRPYENAFSASYAYAWSGWLAVGISHKWIYSKKDLFTAK
                     AQFNDIGIMARLPLVWFNTGGYRNEISAGFSSINNRAGKKPVYKSDYHETIVDPMSND
                     TSFVFFGGDPIKVQRFDYWAFAYGLSYQSDAWRFTVFKGMIQLVYKDANVLDDELRVG
                     TEWTIFDILSLRTQTKNFEKQKNKPRYKEKLIGGAYGASVIIPLKVLDISQWDITPRI
                     DWTRSRGLYEKQTYTYWNFGIQMNFWNTK"
     gene            complement(5641..6528)
                     /gene="atpG"
                     /locus_tag="PK754_09475"
     CDS             complement(5641..6528)
                     /gene="atpG"
                     /locus_tag="PK754_09475"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNB09776.1"
                     /translation="MATLREIKRRIVSVKSTQKITKAMKMVAAAKLRRAQENIISARP
                     YAYKLGALIKHLARNEEVQQNPLIKPRDVKNIMIVVVTADRSLCGAFNTNIIRYVNNL
                     LKGDLKSKLDNNNVKLVTVGRKGDEFFTKRHFPVVFRHANFFNHLNFDDASKVITYCV
                     QEYSAGRVDEVHVVYNEFKSAIQQNLVMERLLPVEPEKVESSNVKYNIETDYIYEPSQ
                     YEIINTLIPRHLNVQMWRILLESFAAEMGARMTAMESATENAGDLIRTLTLYYNRARQ
                     AAITKEILEVVSGAEALRK"
     gene            complement(6538..6897)
                     /locus_tag="PK754_09480"
     CDS             complement(6538..6897)
                     /locus_tag="PK754_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09777.1"
                     /translation="MKRIARIITLIGFGAALYGCAPKAQEVPMETFVDIWIKAQSDSA
                     FKTKYAKPFEEATATDLEAFAKPYGYTGADMKFTTSEIISKNKNKENEFLERMNEATL
                     RTLNEKPDTSAVIESKK"
     gene            complement(6894..7238)
                     /locus_tag="PK754_09485"
     CDS             complement(6894..7238)
                     /locus_tag="PK754_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09778.1"
                     /translation="MRNVLLLLGWVMLAGCGPKEIPAEPMATITYEVMRSKSFLVDDV
                     KFEELPDADLEPIVKKLGFTPSDYRHTVKLFDNDAAKKNDLAVRIGKLLQSDLEKASQ
                     NDSTVFKQLMTK"
     gene            complement(7250..>7877)
                     /locus_tag="PK754_09490"
     CDS             complement(7250..>7877)
                     /locus_tag="PK754_09490"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489342.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNB09779.1"
                     /translation="ERAAKLNQDLGGGSLTALPIIETQASDVSAYIPTNVISITDGQI
                     FLESNLFYSGVRPAINVGISVSRVGGSAQIKAMKQVAGRLRLELAQYRELEAFAKFGS
                     DLDKATQQQLRRGARLVEILKQGQYQPMSFDAQITIIYAATNGFLDDVPVGDCRRFEK
                     ELTETMELRHKDVLSAIMEKKELTDDIKNKLNTIIGDFKAAFKVTKED"
BASE COUNT         1976 a         2015 c         1775 g         2111 t
ORIGIN      
        1 tctctaagac ataaatccct ctttgccggc tttgcgcctt cgcgaggata tatgacacac
       61 tatatagaat aaatccgata atttttttaa agaaaatatt attttttgta ataaatatag
      121 ctctatatgt actactctta tcaatatggg taatctctaa atctgaggga tagatatatg
      181 aagcgccgtt tgtggattca cacgatcatt gctctaagca cgctgatact gacggttgtt
      241 ctgcatcaaa ggataggcag agccggtatt gacgctaaaa cctccttgcg gctccggctc
      301 aaaaaaccgg acatcattga tgcacgcttc atcgccggcg gtaactggta ctatgtgatg
      361 cgcaatcagg gtgctttcat gtatgatcgc ggagactggg ataataatgg gaataatgcc
      421 ggcggcgctt tcccgcgtgg ttcaggcgcc actgtggtct atgccgccgg gccgtggatc
      481 ggtgcgatca aaaacggtgt tccggttgtt agtgaaactc aatttgcttc cgaattccag
      541 cccgggcgca tcctcaactc cggagtgccg tttgaagaac ttatagcgga ctcggtagat
      601 aatccattaa atcaagtgta cgtcgtagat cgtaatacaa cgcctaatga atggacacaa
      661 cttccttcag agtttaaaaa ccaatatggc aactcccctt cactcatcgc cgatgtacaa
      721 gcctggaccg tattcaatga tctggatact accctgtctc aggaaacaag ccactcaagc
      781 ccatttccgg gtttaggcct tgagatcaca ctggaatcca tgatgtatga gacaccattt
      841 ctgaataatt ctgtgctact acactatacc attaccaata aaacgagtac gccttacacc
      901 ggggcttact ttgggctgtg gatggatgcg gatgtggatg ctgctagtaa tgatctggtc
      961 ggttcggact cgacgcgtgg actgggatat atttacaatg cagataacac cgataaacct
     1021 ttagctgtgg gatttgacat cctgcaaggc ccggtggtgc acaaagattc cattccggcg
     1081 caatatgcgt acaagtttca gaataacacg agcgtattac aattaaatcc tatcactaaa
     1141 aaatacgaat ctaaaacgat cagcagtgac agtctggtaa tcggagctat aacagcgaat
     1201 tcttatttcg gagcagctga tccctttaac aatttcgatc gctatacatt acttcagggt
     1261 atcgaacagt atggatatcc ttactttata aacggttggt attttcccgg caaccccgtt
     1321 acaggaacag gatcaataca catggtaggg agtagtcagc gcatcctcgt cgctaccggt
     1381 ccgtttcgta tggaggccgg aggttcgcag gagatatggg ccgccgtagt cggcggtgaa
     1441 ggcgtagatc gcttggatgc cgtagccaat ctctttcaaa atgatgacgt cttgcaatcg
     1501 gcatttacgt cgggcattca ttccttggtt cttccttcac cctctctgag cgtaaccgcc
     1561 ggcgacggct tcgtgacact cacatgggat tccttagccg aagaagcctc cgatcccatc
     1621 gccgcgctgg atatgatcaa taccgataac ggctactccc gtgatctcct cctgcatgac
     1681 ttccagggct accgcctctg gaaaagcctt accgcactgc ccggtcagtt tgatctgatc
     1741 gctcagtttg atctcaatga cggcatccga tctgtccgcc gctggtacct cagtccaagc
     1801 ggtaaactct ccctctcaaa cgtaagcgtc ggcgaagata ccggtatcga atacacctac
     1861 accgatcatg atgtccgcaa tggaagacgc tactactatg cgctcaccgc ctacgatgcc
     1921 cagcctgcgg tctatggccc ggacagtgtg tattatgctg atctggggcg cctcattccc
     1981 aaacccctga tcatgaaccc ttccacagag tcttccctgt gggataatat caccgccgta
     2041 acaccgcagc ctaaaaaagc ggaatctaaa aaggaactct ctcatatctt agtcgtaccc
     2101 aatccttttt atgctaccac gtccgaagga gagcaaatca agttcaccgg actgccggcc
     2161 tcctgcacca ttcgcatctt caccgtggcc ggtgatttag tcaaaacgat aaaacaccac
     2221 gccaccagca acaacaaccg cgtggatgac acactcttga atgaagacac cagtcctttg
     2281 gcacaggaaa catccgtcga ggtatggaat ctaaaaaatg aaaaaaaaca tcccctcgcg
     2341 ccgggcatct acatcgctca tatccattca tcgctcggtg ataaaatcgt caaatttgcc
     2401 gtcatacgat gaaggaagta tgcatgacaa tatctgtggc ctgttcaaag atgtacccgt
     2461 tttctcgcag tgtctctaag acataaatcc ctctttgccg gctttgcgcc ttcgcgagga
     2521 tatatgaaac gctatcgttc ccggaaaata actccgtatt taccgggggc cttgtcccat
     2581 aaaacaaaaa gcccccgcaa tacatgcggg ggcttttgcg tatcaaacac aatcttgttc
     2641 tattgtttgt tgtatttctc tgcaagacgc aaacgattgc gcgaacgaag caacgcctga
     2701 cgcgaacgtt cgggatcgta gctggaacga tcttcaaggc gtttgcgggc gcgttcgtaa
     2761 gactcctgag cgcgaatcaa gtcaatcgaa ctgacttttt cgcaggtctc cgcaagaaaa
     2821 gtgactttat tgtgttccac ttccacaaaa ccaccgctcg ttgcataaat ttcgtcatac
     2881 ccgtccgtgt gacgaatttt gacttcaccg ataagtaccg atgaaatata agccgtgtga
     2941 tttttgagga tctggaaact accactcgca cccggggccg taaaactttc aacttcccct
     3001 ttgaacacga catggcgcgg tgagatgatt tccaaatgat atttattatt gtccatgata
     3061 tttccttcgt ttaccgatta tcggcgcatc ttttccgctt tttcttccgc ttcatctatg
     3121 gtacccacca tatagaaggc ttgttccggc agatcatcca tctgaccttc ggcgatcgct
     3181 ttgaaaccgg caatcgtatc ttctttcttc acatagcggc cgggcgtacc ggtaaactgc
     3241 tctgcgacgt ggaaaggctg agataagaaa cgttgaattt tacgggcacg cgctacgacg
     3301 atcttatcgt catccgacaa ttcgtccata ccgaggatcg cgatgatatc ctgaagatct
     3361 ttatatttct ggagaatttc ctgcacgcgt ttcgccgtat cgtagtgttc ctgacctacg
     3421 acgagcggat caagaatacg cgacgtggaa tccaaaggat ccacagccgg atagataccg
     3481 agctcggaga tcgcacgcga aagcacggtc gtggcatcca agtgtgcaaa cgtcgtcgcc
     3541 ggtgcgggat cggtcaaatc gtcggccggc acatatacgg cctgtacgga tgtgatcgaa
     3601 ccttttttcg ttgatgtgat acgttcctga agttcaccca tttccgttgc aaggttcggt
     3661 tggtaaccca cagcgctcgg catacggccg aggagcgcgg acacttcaga accggcctgc
     3721 gtaaaacgga agatattatc aataaacaga agcacatctc ggccttcgtc atcacgaaag
     3781 aattcggcca tcgtgagggc cgtcaacgca acacgctgac gcgcgccggg cggctcattc
     3841 atctgaccga ataccagcgc ggttttattg agtacaccgg aatctttcat ttcgtgatac
     3901 aaatcatttc cttcgcgggt acgctcgcct acgcctgcaa atacggaata gccgccgtgc
     3961 tgtttggcga tattgtgaat caattcctga atcaacacgg ttttgcctac accggcgccg
     4021 ccgaaaagac cggttttacc accgcgcatg tacggttcga tcaagtcaat aaccttgata
     4081 cccgtttcaa acatttcact tttggtggtc agttcttcta atttcggagc aggacggtga
     4141 atgggcatac gtttgttgga ggcttcgatt ttaccgaatc cgtcaatcgt atcgccggta
     4201 atgttaagca aacggccgag tactttttca ccgaccggta cggcgatcgg agcgcccgtg
     4261 tccacggcat ccattccgcg cacaaggcct tccgtcgcat ccatagcgac cgcacgcaca
     4321 cggttttcgc caaggtgctg ctgcacctcg acaaccagcg tcgaaccgtc gggacgcgta
     4381 acatgagccg aattgtaaat ctccggcaat tggccgttgg caaactcgat gtccaccaca
     4441 gggccgatga tctgaacgat tttacctttg ttcatgagtg tcgatcctta attctatatg
     4501 tgttaaatta tttcgtattc caaaaattca tttgtattcc aaaattccaa tacgtgtacg
     4561 tttgtttttc gtataacccg cgactacgtg tccaatctat acgcggtgta atatcccatt
     4621 gcgatatatc taaaacttta agaggtataa tcaccgatgc accgtacgcg ccgccaatga
     4681 gtttttcttt atatcgcggt ttattttttt gtttttcaaa atttttagtc tgtgtacgca
     4741 aagataatat atcaaatata gtccattccg ttccgacacg cagttcatca tcaagcacat
     4801 tagcatcttt gtaaactaac tgaatcatac ctttgaatac ggtgaagcgc cacgcatcag
     4861 attggtatga aagtccgtac gcaaaagccc agtagtcgaa tcgctgcacc ttgatcggat
     4921 cgccaccgaa aaatacaaaa gaagtatcgt tactcattgg atccacgatg gtctcatggt
     4981 aatcgctctt atacacaggt tttttccctg cccgattatt aatactgcta aatcccgcac
     5041 taatttcgtt gcgataaccg ccagtgttga accatacgag tggaagtctc gccataatac
     5101 cgatatcatt aaattgtgct ttagcggtaa acaaatcttt tttggaataa atccatttat
     5161 gcgaaatacc cacggcaagc caaccggacc atgcatacgc ataagaagcg gaaaatgcgt
     5221 tttcatatgg acgataatat tcgcctgtac catccggatt ttcttcgttg gtacgctcta
     5281 ttcgtggata gttgaagcga taattattta ccgctagtga accgaaacgc gtatgcccca
     5341 tacctgccgt ccagtaattg atcgaggtat tggcgacata gtcattcgtg ttatagctca
     5401 tacccaggcc ggatgcaaat gcaatgcccg ccggattgta aaaagctgac cacgcattgg
     5461 ggatagccac cattggaccg atcgccgcag aacgtgcgtc cggacggatt ccgagaaatg
     5521 taaaaccgac agcattctct tctccggccg tcagacgcgt aaccatcgta gcgactaaaa
     5581 aaacgtaccg tattgtcaaa gaacttcccc taaacatacc ccatccctct acgtaaaaat
     5641 ttacttccgc aaggcctctg caccggaaac aacttccaat atttctttgg tgatcgcggc
     5701 ttgtcgtgca cggttataat acaaggtcaa cgtacgaatc aaatcgcccg cattctccgt
     5761 cgccgactcc atcgccgtca tacgcgcacc catttcggcg gcaaatgatt ccagcagaat
     5821 acgccacatc tgcacgttca gatgacgcgg gatcagcgta ttgatgattt cgtattgcga
     5881 aggttcgtag atataatccg tttcgatgtt gtacttcaca ttggacgatt cgactttttc
     5941 aggttcaacc ggcaaaagtc tttccatcac gagattttgc tgaatcgccg atttgaattc
     6001 gttatacacg acatgcactt catccacacg tccggccgaa tattcctgca cgcaataggt
     6061 gatcactttc gaagcatcgt caaaattgag atgattgaaa aaattggcat gacgaaatac
     6121 gacaggaaaa tgacgtttgg taaaaaattc atcgccttta cgcccgaccg taaccaattt
     6181 tacattattg ttatccaatt tggacttgag gtcgccctta agcaggttat tgacataacg
     6241 aataatgttg gtattgaacg cgccgcacag cgaacgatcg gccgtcacga cgacgatcat
     6301 gatgtttttc acatcgcgcg gtttgatcag cggattttgc tgcacttctt cattgcgtgc
     6361 cagatgtttg atcagcgcac cgagtttgta agcatacgga cgcgcggaaa taatattttc
     6421 ctgcgcacgg cggagtttag ccgccgccac cattttcatg gccttggtga ttttttgcgt
     6481 acttttgacg gatacgatgc gtcgtttgat ttcgcgtaat gtcgccatag tgtgtgttta
     6541 ctttttggat tcgatgaccg ccgatgtgtc gggcttctca ttgagtgtcc gcagcgtcgc
     6601 ttcgttcatc cgttccagaa attcgttttc tttgtttttg tttttgctga tgatttctga
     6661 cgtggtaaac ttcatatccg cgccggtata gccgtacggc tttgcaaatg cttccaaatc
     6721 cgtcgctgtg gcctcttcaa aaggtttggc gtatttggtt ttgaaagcgg aatcactctg
     6781 cgccttgatc cagatgtcca caaatgtttc catcggaact tcctgcgctt tgggcgcaca
     6841 tccgtacaat gccgcgccga agccaatcag agtaataatg cgtgcgatac gtttcatttg
     6901 gtcatcagtt gtttgaaaac agtggaatcg ttctgacttg ctttttccaa gtcgctctgc
     6961 agcaatttgc cgatacgcac agccagatca ttttttttgg ccgcatcgtt gtcaaaaagt
     7021 tttacggtat gacggtaatc cgacggcgta aaaccgagtt ttttgacaat cggttccaga
     7081 tcggcatcgg gcaattcctc gaacttcaca tcatccacga ggaacgattt gctgcgcatg
     7141 acttcgtaag tgatcgtggc catcggttcc gccggtattt ctttgggacc gcaaccggcc
     7201 aacatcaccc aacctaaaag aagcagtacg tttcgcataa agatttccgt tagtcttctt
     7261 ttgtaacttt gaacgcggct ttgaagtcgc cgatgatcgt attcagtttg tttttgatgt
     7321 cgtccgtcag ctctttcttt tccatgatcg ccgacaaaac gtctttgtga cgcagttcca
     7381 tcgtctcggt cagttctttt tcaaaacgac ggcaatcgcc gaccggcaca tcatcaagga
     7441 atccgttggt cgcggcatag atgatggtga tctgcgcatc aaacgacatc ggctgatatt
     7501 ggccttgttt gaggatttcc accaaacgcg caccacggcg aagctgctgc tgtgtcgctt
     7561 tgtcaagatc ggaaccgaat ttagcaaacg cttcgagttc gcgatactgg gccaattcca
     7621 aacgcagacg gccggctact tgtttcatgg ctttgatctg tgcgctgccg cctacgcggg
     7681 acacggaaat accgacgttg atcgcaggac gtacgccgga ataaaacaaa ttggattcga
     7741 gaaatatctg accgtcggtg atcgaaatca cgttggtcgg gatgtacgcc gacacgtcgc
     7801 tcgcctgcgt ttcgatgatc ggcagcgccg tcagcgaacc gccgccgaga tcctgattaa
     7861 gtttcgcggc gcgctca
//
LOCUS       DAOQNQ010000140         7854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3446_length_7854_cov_5.179254, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000140 DAOQNQ010000000
VERSION     DAOQNQ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7854
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3446_length_7854_cov_5.179254"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2845)
                     /locus_tag="PK754_09495"
     CDS             complement(<1..>2845)
                     /locus_tag="PK754_09495"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNB09780.1"
                     /translation="IAINDGSGGFTVLSNVAVGSLPYEIASADFDGDGDLDIAVSNNS
                     SSDISIRSNDGSGNFGVLANISTGVAQLTVAAGDFDGDGDVDLISSESGTQLRVRLND
                     GTGSAFSPGNVINVQNNPYFITVADFDNDGDLDLTASNYSSGTFSVRLNDGTGLFGAG
                     GDFTTNGIQPRAIRAADFNGDGLMDIAFCNENSGNVGIRLNDGVGGFSGNEIGVGSLP
                     QSITLGDFEGDGDMDVAAVNYGSTNVQTIVNDGAGNFVAGSSYGTGNGGVILSSGNFS
                     GTATMEIAAADNTANVIYVLKNAVILPTTTLVTSKGDGGSGSLRDAIDYANSHAGPDT
                     IIFNVSVDSVLLASQLPDITDDSLFINGDYNTDNIPDVVIDGINNGINEAFVRVAAND
                     ITIKGLTIIRFVQGSEAALTISNAARRAKILYNYIGTDGTALYSNVNNIDILAPAGGG
                     QHEIRGNVISARGYGIFFYGSSNDLDSTKIVDNKIGTNAAGSAPLTYAPSHLSGIYIQ
                     NGRGLKIHSNIISGWSGAGALAINFGYTGFSYYAKEVEIFDNKIGTDFSGTAAIPNER
                     GIYFDAGSNANFIGKPGLGNIISGNTGNGIEIWSDSNVIEDNFIGVDISGNAPLANVG
                     MGIQLQNASVNTIGNGNTISGNANHGIFIFSNGGLADENTIINNNIGVGADGVTPVGN
                     NQMGVRIYSLLGARRNLLLDNAIAFNNFHGIEMDGSASADSMIAHGNRIYSNNLGGMN
                     YVAGSNGSITPPMISFMSMDSVVHGTSDPNAFVQIYVDSTNQGRLRIGTGVADGAGNF
                     AVPVTLLGGEAPFLTALQSSTGRTSGFSVPISLTLSDLSVVNSLADDGSPGTLRAAID
                     YANSHVGPDTIRFDISLLGQTINMNSNLPDITDDYTVIDGDVDGDGSGDVQLYGLSPS
                     ADKGLNILSSYNEIRNLRISNFFNNASGIDIDGG"
     assembly_gap    2846..2859
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2860..7822)
                     /locus_tag="PK754_09500"
     CDS             complement(<2860..7822)
                     /locus_tag="PK754_09500"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNB09781.1"
                     /translation="MDIATANGGSSNVSIRLNDGAGNFSGSTDVAVASGAFSVYAADF
                     DGDGDVDVATANPSSNSVSIRLNDGAGNFSGSTNVGVGTGPQSVYVADFDGDGDMDIA
                     VANTGSSTVSIRMNDGAGNFSGTTDVPSGSVPYSVYAADLDGDGDIDIATANFSSNDV
                     SIRLNDGSGNFSGTTDIVVETNPQSVYAADFDGDGDMDIAAANSGSNSVSIRLNDGAG
                     NFSGSTTVYVGSGPSSVYAADFDGDGDMDIATADLSDYVSIRLNDGAGNFSGTTDVAV
                     GWSPLSVYAADFDGDGDMDLATANSVSNDVSILINAPGYFNVTSVTPLQNQGNVGISS
                     NIFVNFPQDINVATLNNATIKIRGSHSGLHTANYFYEASLSIDPDTDFKFGEVVTVTL
                     TDGIIATNTDTLNPYSWQFTVGAFPATGTFTTLPGATVGSTPFGVSLADLDGDGDLDM
                     AAANDVDGTVSIRFNDGTGNFSGSTDLSSGTNSRAVTAADIDNDGDLDLLIASSGFVA
                     SYINNGLGSFSGPNNFAIGGNGQSVFAADFDGDGDVDFVTANSGSNNVSIRLNDGAGN
                     FSGTTDVAVGNLAVGVTGGDVDSDGDIDLLASNYNDNTVSILLNDGAAGFSSGGTSSV
                     SFGPVMVRLADLDADTDLDLVTANEGGSDISVRLNDGTGVFSGGSDISGLVSPRSVFL
                     GDLDGDGDVDIAAGNTGGATTVSIRINDGSANFSGSYNAFVGPGPAWSIDGGDVDNDG
                     DIDIIRVDGSVSGNTTVLKNNNYIVPTSVIPIPNTVGINPTANIVVEYPEDMSSFDAK
                     VFGSLSGYMNTSLFYDNVLYTLTIDPTANFKPGEVVTVIMSGASNVAVSGNILRNIQF
                     QFTAAPSFAAASYSLPASNLAAGTNPGGVATADFNLDGTIDIVATNVSPSEVTIILND
                     GIGGILSSYTVPVGTNPYGPHVADLDNDGDMDIVTADAGGNTVTTLLNDGAGNFSGPS
                     VAVGSGPYAVQSADIDGDGDLDLVVANANSNNVSVLINDGAANFTGGATVSVQTTPYD
                     IVAGDFDSDGDMDFATANVGANSISVRLNDGAGNFPIASDPAVGTNPQGIQAADLDGD
                     GDIDILTANVGSNDASVMLNDGSGNFFGGQTPTVGAFPYSIIASDLDGDGDVDFATAD
                     WNANTVSVRYNDGAANFSGSTAISVGSQPYDIAAADLDGDGSIDLITPNAGSNDVSIA
                     FNDAILPSPSGLYAVAQNGQVELNWSAAPGATLYRIYHDVNPNPMVQTDSSVGTNKTI
                     AGLVNGTLYFFRIKAINATDESGYSNEDAAVPVAGAGQALSLEGIDDEAAIFTSYQNL
                     GVDPSSVTISAWVKPNGTSPSISTIYEGQGIILDAGGYLGLTRGDYTGQDRIWAYLWD
                     GSQIFVGIPYTNGVWTHVAVVLRNGQLYAYKNGVVVDSAVSGNVQNITSGSIGVGVNP
                     YNSLHFEGQIDEMRIWNRGLMDAEMASVQASPSRGDEAGLLANWHFDEGSGSTTFDAS
                     PNNRNLTLFNGPTYVVSGAMAPPLSIVSTTPSANGLSIPANSNIIVNFTSDILTDSLN
                     SRNILIRGAQSGDHTFTYGYGAQTANIDPNTDFIPGELVTVTLTRGILAISSDTLKPY
                     SWQFYVQTNLATGFFSAGDTVVVGTFPKAIAAADFNNDGSLDMA"
BASE COUNT         2034 a         2093 c         1841 g         1872 t
ORIGIN      
        1 acctccgtcg atgtcaatgc cgctggcatt attaaagaaa ttcgaaatcc gtaaattccg
       61 gatctcattg taggaagaca aaatattcaa acctttgtct gctgaaggac tgagaccata
      121 caactgaaca tccccgctgc catcgccgtc cacatcgcca tcgatcaccg tataatcatc
      181 ggtgatatcc gggagattgc tgttcatgtt gatcgtctga ccgagaagcg aaatgtcaaa
      241 ccgaattgtg tcaggaccga cgtggctgtt ggcataatca attgctgcac gaagtgttcc
      301 tggcgatcca tcgtctgcta atgaattaac aacgctcaga tcagaaagtg tcagacttat
      361 cggcacagaa aacccggaag tccgaccggt actgctctgc aatgctgtca gaaatggcgc
      421 ctcaccgcca agcagggtca caggtacggc aaaattgccc gcgccatcag caacgccggt
      481 tccgatgcga agccgcccct gattcgtgga atccacgtag atttgaacga acgcgttggg
      541 gtccgacgtt ccgtgaacca cggaatccat actcatgaaa gaaatcatcg gaggggttat
      601 actgccattg gagccggcaa cgtaattcat gccgccgagg ttgttgctgt agatacggtt
      661 tccgtgggca atcatactgt ccgcgctcgc actgccgtcc atttcgattc cgtggaaatt
      721 attgaaggca atggcattgt ccaacaacag attccgcctg gcgccgagca gcgaatagat
      781 ccgaacgccc atctggttat tgccaacggg ggtaacgcca tcagcaccca cgccgatatt
      841 attattaatg atcgtgtttt cgtccgcaag gccgccattc gagaatatga agataccatg
      901 atttgcgttc ccggaaatcg tgttgccgtt accgatcgta ttaacgcttg cattctgcaa
      961 ttggataccc atcccaacgt ttgcgagagg cgcgttacct gagatgtcca cgccaatgaa
     1021 gttgtcttca atcacgttgg aatctgacca tatttcgatg ccgttgcctg tattgccgga
     1081 gataatgttt ccgagaccgg gcttgccgat aaaattcgcg ttagaacccg catcaaagta
     1141 aattccgcgt tcgttgggaa tcgccgctgt gccgctgaaa tcggtaccga tcttattatc
     1201 gaaaatttct acttcttttg cataatagga aaaaccggtg tagccaaaat tgatggccag
     1261 ggcgcccgcg ccgctccatc cggaaataat attactatga atcttcagac cacgtccgtt
     1321 ttgaatgtag atacccgaaa gatgacttgg ggcataggtt aggggcgccg atccagccgc
     1381 attcgttccg atcttgttgt cgacaatctt ggtcgaatca agatcattgg aactgccgta
     1441 aaaaaatatt ccataacccc gcgctgaaat gacgttgcct cgtatttcgt gttgcccgcc
     1501 gccagctggc gccaggatgt cgatgttgtt cacgttgctg tagagtgccg ttccgtcggt
     1561 accgatataa ttatagagta ttttcgcccg gcgtgccgca ttcgaaatcg tcagtgccgc
     1621 ttcggaaccc tgcacaaacc gaatgatcgt caaacccttg atggtaatat cgtttgcggc
     1681 aacgcgcacg aatgcttcgt tgataccgtt gttgatcccg tcgattacaa cgtcgggaat
     1741 gttgtcggta ttatagtctc cattgataaa tagagaatca tcggtaatgt cgggtaactg
     1801 agaggcgagt aagacggaat cgactgatac attgaaaatg atcgtatcag gaccggcgtg
     1861 agagttggca tagtcgattg cgtcgcgcag tgatccgcta ccgccatccc cttttgatgt
     1921 aaccaaagtt gttgtcggca aaatgaccgc gtttttgaga acatagatta cattcgcagt
     1981 attgtcggcg gcggcaattt ccatggtcgc tgtgcctgaa aaatttcctg atgaaaggat
     2041 aacaccgccg ttgcccgtgc cgtaggaaga acctgctacg aagttaccgg cgccatcatt
     2101 gacgatcgtc tggacattcg tggatccgta gttcacggca gccacgtcca tgtcgccatc
     2161 tccttcaaaa tcacccaaag ttatggattg cggtaatgaa ccgacgccaa tttcgttgcc
     2221 ggagaatcca ccgacgccgt cattcaatct gatccctacg ttaccgctgt tctcattaca
     2281 aaaagcaata tccataagcc cgtcgccgtt gaaatcggcc gcacggatgg ctctcggctg
     2341 gatcccgttg gtcgtaaaat ctccgccggc gccaaaaaga cccgttccat cgttcaagcg
     2401 tacggagaat gtaccgctgc tgtagttcga tgccgtcaga tcgagatcgc cgtcattgtc
     2461 gaaatcggcg actgtaataa agtaaggatt attctgaaca ttgatcacat tgccgggcga
     2521 gaaggccgat cctgtaccgt cgttcaagcg aacgcgcaac tgggttccgc tttccgaaga
     2581 aatcagatcg acatccccgt cgccatcgaa gtcaccggcc gcaacggtca actgagctac
     2641 acccgtgctg atattggcaa gcacaccaaa attgccgctt ccatcgtttg aacgaataga
     2701 tatatccgag ctggaattat tagaaaccgc aatatcgaga tcgccatcgc cgtcaaaatc
     2761 agcagacgcg atctcataag gaagactgcc caccgctacg tttgacaaaa cggtgaagcc
     2821 gcccgagccg tcattgatgg caatgnnnnn nnnnnnnnnc cgccatatcc agacttccgt
     2881 cgttattgaa gtcggcagcc gcaatggctt ttggaaaagt tcccacgaca acggtatcgc
     2941 ccgcggaaaa gaaacccgtc gcaagattcg tctgaacata gaactgccat gaatatggtt
     3001 taagcgtatc gctgcttatg gctaatatac cccgggtcaa agtgacggta acaagttcac
     3061 cgggaataaa atccgtattg gggtcaatat ttgccgtttg cgcaccgtat ccgtatgtaa
     3121 aagtatgatc accgctttgc gcaccacgaa tgagaatatt tctcgaattc agactatccg
     3181 ttagaatatc cgatgtgaaa tttacaataa tattgctgtt tgctggaata gaaagcccat
     3241 tggccgatgg tgtggtcgag acgattgaca gcggaggcgc catcgcgcca gatacaacgt
     3301 atgtagggcc gttgaacagc gtaagatttc tgttatttgg cgaagcgtca aatgtggttg
     3361 aacctgagcc ttcatcaaaa tgccagtttg ccaaaagacc tgcttcgtcc ccacgtgatg
     3421 ggctagcctg aaccgatgcc atttccgcat ccatcaaccc gcggttccag atccgcattt
     3481 catcgatctg accttcaaaa tgcaggctat tgtatggatt cacaccgaca ccgatggagc
     3541 cggatgtaat attctggaca tttccactga ctgcagaatc cacaacaaca ccattcttat
     3601 aggcgtaaag ctgaccattg cgaagaacga ccgccacatg agtccagacg ccgttcgtat
     3661 atggaattcc tacaaatatt tggctcccgt cccatagata ggcccaaata cggtcctgac
     3721 cggtataatc gccgcgcgtt aatccgagat aacctccggc atcaaggata atcccttggc
     3781 cttcatagat cgtggaaatc gacggagacg taccgtttgg cttaacccag gcgctgatcg
     3841 tgaccgatga cgggtcaaca cccaagttct ggtatgacgt aaagatcgcc gcctcatcat
     3901 caataccctc aagactcagc gcctgaccgg cacctgctac cggaaccgcc gcgtcttcat
     3961 tagagtagcc gctttcatcg gtcgcattga ttgctttgat acggaaaaaa tataaagtcc
     4021 cgttaacaag tcctgcgatc gtcttgtttg ttccaactga agaatccgtc tgtaccatag
     4081 gattgggatt gacgtcatga taaatgcggt ataatgttgc gccaggtgca gcgctccagt
     4141 tcaattcaac ctgcccattt tgagctaccg catataaacc gcttggtgac ggtagaatcg
     4201 catcattgaa tgcaatcgag acatcattgg agcccgcatt gggcgtaatc agatcgatag
     4261 aaccgtctcc gtcaagatcc gctgcggcaa tatcataagg ctgtgatccg acggagatcg
     4321 ctgtgcttcc tgaaaaattg gccgcgccgt cgttataacg tacagaaacc gtattggcat
     4381 tccaatctgc cgtagcaaaa tccacatcgc catcgccgtc aagatcactg gcaattatgg
     4441 agtaaggaaa agcaccgacc gttggggttt gaccaccgaa aaagtttcct gacccgtcat
     4501 ttaacattac cgatgcatca ttggatccta cgtttgccgt cagaatatcg atatctccat
     4561 cgccatccaa atctgccgcc tgaatgccct gcggattggt tccaacagcc ggatcggatg
     4621 caatcgggaa attacccgcg ccgtcattta gacgaaccga tatgctgttt gcccccacat
     4681 tggccgtcgc aaaatccata tcgccgtcgc tgtcaaaatc tccggcgaca atatcatacg
     4741 gcgttgtttg gacagaaacc gtagcgccgc cggtaaaatt tgccgcgccg tcgttgatca
     4801 aaacgctcac attatttgaa ttcgcgttcg ccacaaccag atcaaggtcc ccgtcgccgt
     4861 caatatcagc ggattgaaca gcatacggac cggagcctac tgcaacactt ggtcctgaaa
     4921 aattaccggc tccgtcgtta agcagtgtcg taacagtatt accgccggcg tcggcggtaa
     4981 caatatccat atcgccgtca ttgtcgagat cggccacatg aggaccgtac ggattggttc
     5041 caaccggaac cgtataactt gataaaattc cgccgatgcc gtcgttaaga atgatcgtga
     5101 cttcactcgg tgaaacatta gtggccacga tatcgatcgt accatccagg ttaaaatctg
     5161 ccgtcgcaac gccgccaggg tttgtcccgg cggcaagatt tgatgccggt aaagaatagg
     5221 acgccgcggc aaaggacggc gcggccgtaa attgaaattg aatattcctc aaaatatttc
     5281 cgctgacggc gacatttgaa gcgccagaca tgatcacagt aaccacttca cccggtttaa
     5341 aattggccgt cggatcaatc gtcaaagtat acaagacatt atcataaaac agcgatgtat
     5401 tcatatagcc gctcagcgaa ccaaaaactt tggcgtcgaa tgaactcata tcttcaggat
     5461 attccaccac aatgttggcc gtaggattga ttccaacggt gtttgggatc ggaatgacag
     5521 aagtaggaac gatataattg ttatttttta agactgtcgt atttccgctg accgaaccgt
     5581 cgacgcggat tatatcgatg tcgccgtcat tgtctacgtc tcctccatcg attgaccatg
     5641 caggccctgg cccgacgaac gcgttataag aacccgaaaa gttggcggac ccgtcattaa
     5701 tgcgaatcga aactgtagtt gccccgcctg tatttccggc cgcgatgtcc acatcgccgt
     5761 cgccgtcaag atcaccgaga aatactgagc gtggagaaac cagaccgcta atgtcggaac
     5821 caccggaaaa aacacctgtg ccgtcgttga ggcggacgga tatatcgctt ccgccctcgt
     5881 tggccgtgac cagatctaga tcggtatcgg cgtccagatc agccagtctg accatgaccg
     5941 gaccaaaact tacagaagaa gtccctccgg aagaaaaacc cgccgcgccg tcgttgagca
     6001 aaatggatac tgtgttgtcg ttataattcg acgcaagcag gtcaatgtcc ccatcgctgt
     6061 caacatctcc tcccgttacg ccgaccgcaa gatttcccac cgccacatcg gtagttccgg
     6121 aaaaattacc tgcgccgtcg ttgagacgga tcgacacgtt attcgatccg ctgttggccg
     6181 tgacaaaatc aacatcgccg tcgccgtcaa agtccgctgc aaaaacagat tggccgtttc
     6241 ctccgatcgc aaaattattt gggcctgaaa aactacctaa accattattg atataggacg
     6301 ctacaaaacc ggaggaagct attaataggt ccaaatcacc gtcattgtca atatcggcgg
     6361 ctgtcacggc tcttgaattt gttccggatg aaagatcagt actgccggaa aaattgcctg
     6421 ttccatcatt gaaccgaatc gagacagttc catcgacatc attggccgca gccatatcaa
     6481 gatcaccgtc cccgtccaga tcggccagac tgactccgaa cggagtggag ccgaccgttg
     6541 ctccgggcag cgtggtgaaa gttcccgtcg cagggaaagc accaacggta aattgccagg
     6601 aataggggtt gagcgtatcc gtgttggtag cgatgatacc gtcggtgagt gtgaccgtta
     6661 ctacttcacc aaatttgaaa tcggtatcgg gatcgatgga taagctggct tcgtaaaagt
     6721 aattagccgt atgtaacccg ctgtgggaac cgcggatctt gatcgtggca ttatttaaag
     6781 tggccacatt gatatcttga ggaaaattga cgaagatatt cgatgaaatg cctacattac
     6841 cctgattttg cagcggagtt acggatgtaa cattgaaata accgggagca tttattagga
     6901 tcgaaacatc atttgaaacg ctgttcgcgg ttgcaagatc catatcccca tccccgtcaa
     6961 agtccgccgc atagacggaa aggggactcc accccaccgc gacatcggta gttcctgaga
     7021 aattgcctgc gccgtcattg aggcgtatcg aaacataatc actcaaatct gccgtcgcga
     7081 tgtccatatc cccatccccg tcaaaatccg ccgcatagac tgaagatgga ccactcccaa
     7141 catatacagt ggtacttccg gaaaaattac cggcgccgtc attcaggcgt atcgaaacac
     7201 tatttgaacc actattggcc gcagcgatgt ccatgtcccc gtcgccgtca aaatctgcgg
     7261 catacactga ttgtggattt gtttccacca cgatatcggt cgtccctgaa aagttccctg
     7321 acccgtcatt taggcgtatc gaaacatcat ttgagctgaa gttagccgtc gcgatatcta
     7381 tgtctccgtc gccgtcaaga tctgccgcat aaactgaata cggtaccgac ccagacggaa
     7441 catcagtggt ccccgaaaaa ttacccgcac catcattcat tcgaatggat accgtactcg
     7501 aacctgtgtt tgccaccgct atgtccatgt cgccgtcgcc atcaaaatcc gccacataga
     7561 cggattgtgg tcctgtgcca accccgacat tcgtgcttcc ggaaaaattt ccagcgccgt
     7621 cattcaggcg tatcgaaaca ctatttgaac ttggatttgc tgtcgcgacg tccacgtccc
     7681 catcgccgtc aaaatctgcg gcatacactg aaaatgcgcc tgacgcaacc gcgacatcgg
     7741 tgcttcccga aaaattacct gctccgtcat tcaggcgtat cgaaacatta cttgaacccc
     7801 cgtttgccgt cgcgatatcc atatccccat ccccgtcaaa gtccgccgca taga
//
LOCUS       DAOQNQ010000141         7851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3449_length_7851_cov_6.473576, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000141 DAOQNQ010000000
VERSION     DAOQNQ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7851
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3449_length_7851_cov_6.473576"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PK754_09505"
     CDS             <1..268
                     /locus_tag="PK754_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HNB09782.1"
                     /translation="KAIDRSPCGTGTSARMAQWAAKGKLKTGDTFVHESIIGSMFRGR
                     VERTTHVGDYDAIVPSIEGWAYITGYNTIFIDTDDPYAYGFQVM"
     gene            314..1573
                     /locus_tag="PK754_09510"
     CDS             314..1573
                     /locus_tag="PK754_09510"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB09783.1"
                     /translation="MSSKKTDVAIIGGGVIGLCTAYYLNKAGISVTIIEKKQPGFGSS
                     FGNAGLISPSHFVPLAAPGMVAKGLKWMFDAESPFYIHPRPDIDLITWLWKFMRASTD
                     KRMRPAMPVLRDMTLASQQLFHTIANEERFDFALEQKGLILLHDTAEGEKAHHHEVAL
                     ANEVGVTAHMISREEIHQMQPGVKVRATGGVFFPQDGHLIPDQFNAKLTSLLQTRGVQ
                     FMNETDVFGFVPNGKAAGHLRTSEGDIHADQYVVAAGAWTSDLLYEIGIALPVQAGKG
                     YSVTVKREKNNLTIPIICTEARVAITPMGDTIRYAGTLELSGNDLTINPRRVKAILRA
                     ADRYLKNFDAQSSDIQTAWAGLRPCSPDGLPFIGRFKRAPNVIAATGHAMLGITLGPI
                     TGKLVSEIVTNTPQTITSPLLDADRYA"
     gene            1566..2633
                     /locus_tag="PK754_09515"
     CDS             1566..2633
                     /locus_tag="PK754_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HNB09784.1"
                     /translation="MPEKKSEFEKRLRTMQRWEAPSSWRCIETIEAHTGGEPLRIIIS
                     GLPPIKGQTILQKRAYLKTHLDHLRTALMWEPRGHADMYGCIITPPVAKNSDFGVIFL
                     HNEGYSTMCGHGIIAVTKAALETGLIRANGTEVTLRIDSPAGLITSIAQIRRGKVQSV
                     RFLNVPSFVQTLDAVVDVPGLGRIRYDLAYGGAFYAFVHAEDASVTMDAAGYRGLIER
                     GMMIKRAVMRSVAMQHPFEPELNFLYGTIFTGPGHTTGADSRNVCIFADGEVDRSPTG
                     TGVSARMAIHHARGALRIGEPMAIESITGSRFVCRVHATTTYGNYPAVIPAIEGTAHL
                     CGKSSWIIDPADSLRDGFFFR"
     gene            2639..3607
                     /locus_tag="PK754_09520"
     CDS             2639..3607
                     /locus_tag="PK754_09520"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HNB09785.1"
                     /translation="MRFISSDTLRELITMPEAIVAMQSAFSALSDGSAVVPLRTTLDF
                     PGSDARALIMPAYHSSSQYFTTKIVTVCPTNAARHLPLIHGTLIVTDGSTGKTLALMD
                     AEWITALRTGAASGLATEWMALPDAHTLGVFGTGAQAYEQVRGVLSVRKLTRIIIFGR
                     YPQKTTTFCKTISNQFAIPCFPAQTPQEVSQCDILCCATNAHNPVFEDKYLTHGTHIN
                     GIGAFKPAMREIPSATIARARIIVDQRASAWREAGDLILAQKEGVVSEDCIAGEIGEV
                     VTHKASGRISREQITIFKSVGNAIQDNVIASMAYERTLKAGVGTEI"
     gene            complement(3685..4419)
                     /locus_tag="PK754_09525"
     CDS             complement(3685..4419)
                     /locus_tag="PK754_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09786.1"
                     /translation="MSKNIKCCVIIFSLLQIGCGGISIIEHQDAETLNRGKFSVLTSA
                     SGGPEEWSKKPRDRFVKAIAPAILEPRLSYGISDRLNVGASLWSGYAAPVLLSKVVLG
                     ANIKSCDFGLRGMLKYKVTPDNSKMQMSLLLNYTNYFLFYSERTDKLASGISPGLIIS
                     KRSSQTSVMYGIIKYNIFNIDHDYGEGGKGSNPNNFPSFHIGYSENINKNKGFAELVY
                     SYVNNPLSIGHTGHVFSISVGYMWKL"
     gene            complement(4554..5138)
                     /locus_tag="PK754_09530"
     CDS             complement(4554..5138)
                     /locus_tag="PK754_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09787.1"
                     /translation="MSNKISIGLIQIALLLIFTDTIYCQTQALNDKRSAFGFGELGIG
                     FSEFGSAYYGGVTFFAGHNALKILVQRNKEIEGWIEWTERLPKEKLNQFCLMYGLRHS
                     SRRVTVAGFVGVSKLMGQIRGKHLYNQSEDENGDDWFAWFPDYAVYEKVKIKNYALSA
                     EGQIIFSFVGIKLFGTFAQKVSYVGAGLSLTLGH"
     gene            complement(5476..6237)
                     /locus_tag="PK754_09535"
     CDS             complement(5476..6237)
                     /locus_tag="PK754_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09788.1"
                     /translation="MIAAAISIRVILDSPGYDSWARVSAHIAVRGANSRKFSDTLRHS
                     TADSREPFDAHQFACLYLKLRMKRVIWTLLIAFLASSINAQEIFLRISKDIDGKHISE
                     STNFWDLKEPLTEKVHSIQFSGEMLFPVNKYFFIGGGMSKSTTMATKRWGGKFSVRPF
                     YGALQIKLSNHMNHVYFGFQYGVSVLSNNVSYEGGGTIKDGSYFGISTGLNFFFLNFD
                     IGYQRHKARWKFEDEKMNIKYNHVALGIGLRLKLK"
     gene            complement(6234..6704)
                     /locus_tag="PK754_09540"
     CDS             complement(6234..6704)
                     /locus_tag="PK754_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09789.1"
                     /translation="MKSFLIKSLATIVIIFSALQACSDDKENNIASIPNTKWKLYKQN
                     KSLYEGEFYMQFTADTQKEWSYDDEGDCIDYEGETSYKVENGLLRSWNSEENTFDSGV
                     EFSITNNTLYIDSILVGKQKIAVFAEYKYNDFDESKFNLCDIFKIKALCRRLTP"
     gene            complement(7008..>7851)
                     /locus_tag="PK754_09545"
     CDS             complement(7008..>7851)
                     /locus_tag="PK754_09545"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNB09790.1"
                     /translation="KGLQIPRAAARHIVANRYLPSTYYRFYIMQILKILSVFFLFLVQ
                     VNAQDQIGVVEMYLGGNYLSMSSTISELNKIKKNTSNPYSFNYSVKHFISNAEIMLGY
                     RVSRRIDVLVSSEYFSPVYSTTTNTPNSEELEIKKKYKYSITPISIGARLNLYEILNK
                     LNPYIEVGYTYYHSKETTSSKGYNYSQIFGYTKIDTSVYVKGNGWGYSVATGIRFNFK
                     SQFSILMRLKYRIASAFSDEKDQLKIHPAKQMKALGEISTSKPLTKYDGFCFAIGLSY
                     NFLK"
BASE COUNT         2245 a         1803 c         1575 g         2228 t
ORIGIN      
        1 taaagcaatt gaccgctcac cctgcggcac aggcacttcc gcgcgcatgg cccagtgggc
       61 ggccaaagga aaactcaaaa ccggcgatac gtttgtacat gaaagtatca tcggaagtat
      121 gtttcgcggc cgcgtggaac gcacaacgca tgtcggcgat tacgatgcca tcgttcccag
      181 catcgaagga tgggcttaca tcacaggtta caatacgatt tttatagata cggatgatcc
      241 ctacgcatac ggttttcaag ttatgtaacg ggatacggta aacatataga aaaaatttaa
      301 ttcggatacg caaatgagtt caaaaaaaac agatgtcgcc atcatcggcg gaggtgtgat
      361 cggattatgt acagcgtatt acctcaataa agccggcatc tcggttacga tcatcgaaaa
      421 aaaacaaccc ggctttggtt cgtctttcgg caatgccggt ctcatcagtc ctagtcattt
      481 cgttcctctg gcggcgccgg gtatggttgc caaaggcctc aaatggatgt ttgatgcaga
      541 aagcccgttt tatattcatc cgcgtccgga cattgatctt atcacatggc tgtggaagtt
      601 tatgcgcgcg agtaccgata agcggatgcg tccggctatg ccggtattac gcgatatgac
      661 attagccagt cagcagcttt ttcataccat tgccaatgaa gagcgttttg attttgcttt
      721 ggaacaaaaa ggcctcattc ttttgcatga taccgccgaa ggtgaaaaag cgcatcatca
      781 cgaagtagct ttagccaatg aagtcggcgt tacggcacat atgatctcgc gggaggagat
      841 tcaccaaatg caacccggcg taaaagtgcg cgccacgggc ggcgtatttt ttccccaaga
      901 cgggcatctc atccccgatc agtttaacgc caagctcacg tcgttattgc aaacacgcgg
      961 tgtgcagttt atgaacgaaa ccgatgtatt tggctttgtc cccaacggca aagcggcggg
     1021 acatctgcgt accagcgagg gcgacatcca tgcagatcag tatgtcgtcg cggccggcgc
     1081 gtggacgtct gatctgctct acgaaatcgg tattgcgctc ccggtgcaag ccggcaaggg
     1141 ttatagcgtc acggttaaac gcgaaaagaa taatcttacg attccgatca tttgtaccga
     1201 agcgcgtgta gccatcactc caatgggcga tacgattcgt tacgccggta cgctggagtt
     1261 gagcggcaac gacctgacca tcaatccgcg tcgtgtgaag gcgatacttc gggccgcaga
     1321 ccgctatcta aaaaattttg atgcgcaatc gtcagatata caaacggcgt gggccggtct
     1381 tcgcccgtgt tcaccggacg gattaccttt catcggccga tttaaacgcg cccccaacgt
     1441 aatcgctgcg accggtcatg cgatgctggg tattaccctt ggaccgatta cggggaaact
     1501 ggtctccgaa attgttacca atacgccaca gaccattact tcgccattat tggatgctga
     1561 ccgctatgcc tgaaaaaaaa tcggaatttg aaaagcgcct gcgaacgatg cagcgatggg
     1621 aagcgccttc atcgtggcga tgtatcgaaa cgatcgaagc tcataccggc ggtgaaccgc
     1681 tccgcatcat catatcagga ttaccgccga tcaaaggcca aaccattctt cagaaacggg
     1741 cgtacctcaa gacgcatctt gatcatctcc gtacggcgct gatgtgggaa ccgcgcgggc
     1801 atgccgatat gtacggctgt atcatcactc cgccggtggc aaaaaattcc gatttcggtg
     1861 tgattttttt gcataacgaa ggttattcga ccatgtgcgg tcacggcatc atcgcagtta
     1921 ccaaagcggc cctagaaacc gggcttattc gtgcaaacgg tacggaagtc acactgcgca
     1981 ttgattcacc cgcaggactg atcacttcca tagcccaaat ccggcgcggc aaagtacaat
     2041 ccgttcgttt tctcaatgtt ccatcgtttg tacaaacttt ggatgccgtc gtcgacgtac
     2101 cgggattggg acgcatacgt tatgatcttg cgtacggcgg tgcattttac gcttttgtgc
     2161 acgccgaaga tgccagtgta acgatggatg cggcaggata tcgcggcctt atcgaacgcg
     2221 gcatgatgat caaacgcgct gttatgcgga gcgtagccat gcaacaccca ttcgaaccgg
     2281 aactcaattt tttatacggt acgattttca ccggcccggg tcatacgacc ggcgcagatt
     2341 cccgcaatgt ctgtattttt gccgacggcg aagtggatcg aagcccgaca ggaacgggag
     2401 tcagcgcgcg aatggctatt caccatgcac gcggtgcgct tcgcatcggt gaaccgatgg
     2461 ccatcgaaag cattacaggc agtcgctttg tatgccgcgt tcatgcgacg acgacgtatg
     2521 gtaactaccc ggcggtgatc cccgctatcg aaggcacggc gcatctctgc ggaaaaagct
     2581 cctggattat agatcctgcc gattcgctgc gggacggttt tttcttccgc taaaatccat
     2641 gcgatttatt tcaagcgaca cactgcgtga gttgatcact atgcctgaag cgattgtggc
     2701 aatgcaatcg gcattttctg cattatccga tgggtcggct gttgtgccgc ttcgaacgac
     2761 ccttgacttt cccggttccg acgcccgtgc gctgatcatg ccggcttatc attcatcatc
     2821 tcagtatttc acgaccaaaa tagtgacggt atgtccgacc aatgccgcgc gccatcttcc
     2881 gctcatccac ggtacactga tcgttacgga cggatccaca ggaaaaacac tggcactgat
     2941 ggatgcagag tggattacgg ctttgcggac tggtgcggct tccggactgg ccacggaatg
     3001 gatggctctt cccgatgcgc atacattagg agttttcggg acaggtgcgc aggcctacga
     3061 acaggtgcgt ggcgtcttga gtgtaagaaa attaacacgg ataattatat ttggccgcta
     3121 tccccaaaaa acaactacgt tttgtaaaac tatttcgaat caatttgcaa ttccttgttt
     3181 tcccgctcaa acgccgcaag aggtttccca atgtgatatt ctctgctgcg caaccaatgc
     3241 gcataacccg gtttttgaag ataagtatct cacgcacggt acgcacatca atggtatcgg
     3301 cgcattcaaa cccgcaatgc gtgaaatacc atcagcgact atagcacgcg ctcgcattat
     3361 cgtagatcaa cgcgcatcgg catggcgtga agccggcgac ttgatcctcg ctcaaaaaga
     3421 aggagtagtc tctgaagatt gcattgccgg tgaaatcggc gaagttgtca cgcataaagc
     3481 ttccggacgt atatcccgag aacaaatcac catcttcaag tccgtaggta atgcgattca
     3541 ggataatgta attgcatcca tggcgtatga acgtaccctc aaagcgggag taggaacgga
     3601 gatttgaacc tacgattagt attagaaaac aacttgttca tattcgaaat atatatttgt
     3661 tttgacatgc cgtagaccat gacttcataa tttccacatg taaccaacgc tgatgctaaa
     3721 aacatgacct gtatgaccta tacttaatgg gttattcacg tacgaataga ccaattcggc
     3781 gaaaccttta ttcttattta tattttcgct ataacctata tgaaaagaag ggaagttatt
     3841 aggattagaa cctttcccac cttcaccata atcatgatct atattaaata tgttatattt
     3901 aattattcca tacataacac ttgtttgtga gctgcgcttc gagataatta atcccgggga
     3961 aatacccgag gcaagtttat ccgttcgttc agaataaaat aaaaaataat tagtatagtt
     4021 cagcaaaaga gacatttgca ttttcgaatt atcaggagta actttatatt ttaacattcc
     4081 tcttaagcca aaatcacaac tttttatatt cgcacccaac acaactttag agagtaacac
     4141 cggtgcggca tacccactcc acaaacttgc ccctacattt aggcggtcac ttataccata
     4201 cgataaccta ggttcaagta ttgcgggtgc aatcgctttt acaaaacgat ctcttggttt
     4261 ttttgaccat tcttctggac cacctgaagc gctagtcagt acactgaatt tcccacgatt
     4321 taatgtttcc gcatcttgat gttcaattat acttataccg ccacaaccaa tttgaagtaa
     4381 ggaaaaaata attacacagc atttaatatt tttactcatc gttgcccgtt tttatagaat
     4441 acaaccgcat ggtacataac ggttcgcgac tatctgcgat ggtgcggtat aaggtttcga
     4501 aaatttcaaa ccactcgtgt ctcgcgccac gatgtatgtg aagtgcgaac gagtcaatgc
     4561 cctagcgtca aactcaatcc agcacccaca taacttactt tttgagcaaa agtaccaaac
     4621 aacttaatcc cgacaaacga gaatattatt tgaccctctg ctgaaagcgc atagtttttt
     4681 atcttcactt tttcataaac agcataatct ggaaaccaag cgaaccaatc atctccattt
     4741 tcatcctcac tttgattata aagatgctta cctcttattt ggcccattaa cttactaacc
     4801 ccaacaaatc ctgcgacggt cactcgccta gatgaatgcc tcaatccata cattaagcaa
     4861 aattgattga gcttttcttt gggtaatctt tcggtccact caatccaacc ttcaatttct
     4921 ttgttacgct gaacaagaat tttgagtgcg ttatgccccg caaaaaaagt cactcctcca
     4981 taatacgctg acccaaattc agaaaatccg attcctaact ctccaaaccc aaatgctgaa
     5041 cgcttgtcat ttagagcctg cgtttgacaa taaatagtat cagtaaatat gagtaacaga
     5101 gcgatttgaa ttaaaccaat tgaaattttg ttcgacatga ttagttcccc cgtgaagttt
     5161 tataaacaca gcaaggggca acatctatac caaagctaac attattaaaa tcaaaaaggc
     5221 gattgtaata aatctttaat tatttttcac actgcaagaa atttattgca gaaacgcaat
     5281 cgcgcttaaa ggcgatctat agacaatgtg tcgtgggacg gagtatttga aaactagcac
     5341 ttactccttc atcgcggcgc gatatatacg atctgcgtga ctaatttatt gttacattca
     5401 tctataaaaa gccgattctc aatactctat gatcagcata cgtacagatc accggaaggt
     5461 tagacgctca tcaatttatt tcaatttcaa tctcaaacca attcctagtg caacatggtt
     5521 atattttatg ttcatcttct catcctcaaa cttccatctc gctttatgcc gttgataacc
     5581 aatatcaaaa tttaagaaaa agaagtttaa acccgtagaa atcccaaaat atgaaccatc
     5641 tttgatcgtt cctccccctt cataagaaac attattagat agtactgaaa ccccatattg
     5701 aaagccaaaa tacacgtggt tcatgtggtt cgataactta atttgcaggg caccataaaa
     5761 tggtcttacg ctaaatttcc ctccccatct tttagtcgcc attgtggtac ttttgctcat
     5821 accaccacct ataaaaaaat atttgtttac cggaaagagc atttcacccg aaaattgtat
     5881 tgaatgtact ttctcagtta atggctcttt taaatcccaa aagtttgttg actctgatat
     5941 atgttttcca tcaatatctt ttgagattct taaaaagatt tcttgagcat tgattgaaga
     6001 tgctaaaaat gcgattaaaa gtgtccagat tactcttttc attcttagtt ttaaatataa
     6061 acatgcaaat tgatgtgcgt caaacggttc gcgactatct gcggtgctgt ggcgcagggt
     6121 atctgaaaat tttcgactat tagcccctcg caccgcgata tgtgccgaga cgcgtgccca
     6181 gctatcgtat ccgggagaat ctaatattac acgtatcgag atcgctgccg ctatcatgga
     6241 gttaggcgtc tgcacagcgc ttttatttta aaaatatcac ataaattaaa ctttgattca
     6301 tcaaaatcat tgtacttata ctctgcaaaa acagcaattt tttgtttgcc aactaaaata
     6361 ctatcaatat ataatgtatt atttgtaata ctaaactcta ctcctgaatc aaatgtattt
     6421 tcttcgctat tccaactcct taataaacca ttttcaactt tatacgaggt ctccccttca
     6481 taatcaatac aatcaccctc atcatcataa ctccattctt tttgagtatc ggctgtaaac
     6541 tgcatgtaaa attcgccctc gtaaagagac ttgttttgct tgtataactt ccattttgta
     6601 tttggaatag atgcaatgtt gttttcttta tcatcactac aggcttgcaa agcactaaaa
     6661 attataacga ttgttgcgag gcttttgata aggaacgact tcatacaatc tcctaattta
     6721 aaactattta tatagtggaa tttcgatcat aaatacattt gagtatgtgt ttagtaaatg
     6781 gattggcggt caatgctgca tgacgcctaa cggtctccgg tatctgcggt gctgtggcgc
     6841 gaggtatatg aaaaactcaa acctctcgta cctcgctcca cagtatcggc acgatgcgag
     6901 ttacaattat cgttagaaat ctaatcatta cctacaaaaa ccctgttttc aataatctaa
     6961 gagcagcaag cgaacggatc accggatggt tatctgccgt ccacttctta ttttaaaaaa
     7021 ttgtacgata gcccgattgc aaaacaaaaa ccatcgtatt tggtcaaagg tttagatgta
     7081 ctaatttcac caagggcttt catttgctta gcaggatgga ttttaagttg atccttttca
     7141 tctgaaaaag cgctagctat acgatatttc aatcgcatta atattgaaaa ctgtgattta
     7201 aaattaaatc taatccctgt tgcaactgag tagccccaac cgtttccttt cacatagact
     7261 gatgtgtcaa tcttcgtata tccaaatatt tgagaataat tataaccttt actagaagta
     7321 gtttctttag aatgataata tgtatatccc acttcgatat aaggatttaa tttatttaaa
     7381 atttcatata agtttaatct agctccaatg cttattggtg tgattgaata tttatatttt
     7441 ttcttaattt ccaattcttc gctatttggt gtattggttg tggtgctgta aacgggagaa
     7501 aaatattcac ttgaaactaa cacatctata cggcgactaa ctctataacc tagcataatt
     7561 tcagcatttg atataaagtg tttaacagaa taattaaatg aatatggatt agaagtgttc
     7621 tttttaattt tatttagctc actaatggta ctactcatag ataaataatt accacccaaa
     7681 tacatttcga cgacaccaat ctgatcctgc gcatttactt gcactaaaaa caagaaaaag
     7741 actgaaagaa tttttagaat ttgcatgata taaaacctat aataagtgga tggcagataa
     7801 cggttcgcga ctatgtgacg tgctgccgcg cgaggtatct gcaatccctt a
//
LOCUS       DAOQNQ010000142         7793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3505_length_7793_cov_5.886017, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000142 DAOQNQ010000000
VERSION     DAOQNQ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7793
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3505_length_7793_cov_5.886017"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..1214
                     /gene="hemH"
                     /locus_tag="PK754_09550"
     CDS             213..1214
                     /gene="hemH"
                     /locus_tag="PK754_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNB09791.1"
                     /translation="MAQKTAIVLLQLGGPDNLDAVEPFLYNLFCDPDIIDLPGAFLFR
                     KGLARLISSRRSPAVRELYKNIGGRSPILPQTEEQAQALQLRLDTSGHQCTVFIAMRY
                     WHPMTDAIARSIAQGGYDRILLLPLYPHYCKATTGSGINEWRRCAKKYQIDHIPTQTV
                     ASYFDHPDYIHAIVDNIKKALTQLPEDARAKTHLVFSAHGTPMKLVRDGDPYSIHIRR
                     TYEAVLSAGNFGLPHHLCFQSKVGPQQWLEPSLVQTIADLSAQKVSHMLVVPVAFVTE
                     HIETLSEINIEVRHEAEQAGVRYFDMMPALRDHPSFTACLYDLTLKALHGAWPVSQS"
     gene            1344..2252
                     /locus_tag="PK754_09555"
     CDS             1344..2252
                     /locus_tag="PK754_09555"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNB09792.1"
                     /translation="MRYVDRFPDPHSRRFDKAEWRDEHGTANVIIHCQSSGIAFPKHW
                     GPLSLKTTLSGHEYYVTRHSTHRVTPERLLILNEDTTYASYIRDGETAESFTINFSAD
                     FIRTVLRDSLSDGMQKLDNPFSDDAPSVQFPEYTLPYHHAIRVRLNRIRRCLTMPADL
                     NGRVDEELSFLYEAMCTLSVSENKKIRDLPFQKASTREEMYRRLQRVRDYMDACYAEP
                     INLHDLSRVACLSVHHLIRLFKSHFLCTPHQYLIRCRLLEAKRLTETTSLRIEDIARR
                     VGFEDRSSFSRLFRHHFDKNPSDRTQ"
     gene            2337..2831
                     /locus_tag="PK754_09560"
     CDS             2337..2831
                     /locus_tag="PK754_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09793.1"
                     /translation="MLMRSFIVSLFFIWTKSFAQMPMDSSAFDFWVGEWTATWSNADG
                     STGKGKNRIVKILDGKVIQEHFEDESGFKGTSISVYTPAQKKWRQAWADNQGGYFDFE
                     GDISDGKKIFKTKMHEANGKQYMSRMLFYDIKTDAFTWDWEKTEDGGKTWALQWRIQY
                     ARKK"
     gene            complement(2851..3906)
                     /gene="arsB"
                     /locus_tag="PK754_09565"
     CDS             complement(2851..3906)
                     /gene="arsB"
                     /locus_tag="PK754_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017316582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNB09794.1"
                     /translation="MEKVSKLSFIDRYLTLWIFIAMLIGVFSGYFFSSIVGFWNRFQS
                     GMTNIPIAIGLILMMYPPLAKVKYEELADVFRNKKVLLLSLVQNWLIGPVFMFLLAVV
                     FLRDYPEYMAGLVMIGLARCIAMVIVWNELAHGDTEYAAGLVAFNSIFQVLFFPVYAY
                     LFLTLFPSLLGLQAFAVDITIMQIAESVFIYLGIPFLAGMLTRMILLKAKGRVWYHEK
                     FIPKISPLTFIALLFTIVVMFSLKGEYIVTIPFDVLRITVPLVIYFVVMFLISFYLSR
                     RSGADYAKSATLSFTAASNNFELAIAVAIAVFGISSGAAFAAVIGPLVEVPVLVSLVR
                     VALWIKSKYYDVPTGAN"
     gene            complement(3931..4353)
                     /locus_tag="PK754_09570"
     CDS             complement(3931..4353)
                     /locus_tag="PK754_09570"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNB09795.1"
                     /translation="MKKRVLILCTGNSCRSQMAEGFLKSFDASLEVYSAGTRPSSQVH
                     PKAVKVMGEIGIDLSGNYPKSTDLFLDKPFDYVITVCGNAQEQCPVFTGTVSHRLHIG
                     FDDPAEASGAEEHVMKEFRRIRDEIKKDFFEFYQKSLR"
     gene            complement(4395..4739)
                     /locus_tag="PK754_09575"
     CDS             complement(4395..4739)
                     /locus_tag="PK754_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNB09796.1"
                     /translation="MTIQNYAEVFKALSDPNRLRILKMLEQRTLCVCEIRDVLKLAPS
                     TVSQHLKILKKNAYITEEKKGRWVYYSLNTKPLYPEITALRILLHTALNDTAQVQSDV
                     KKIQSSKVITCS"
     gene            4825..5013
                     /locus_tag="PK754_09580"
     CDS             4825..5013
                     /locus_tag="PK754_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09797.1"
                     /translation="MRIIEVMLDNAIRTILICAMQRIFKSSLFFTVFVLLSMASHAYS
                     ASQQNETGFPHKTTQEPT"
     gene            5271..5441
                     /locus_tag="PK754_09585"
     CDS             5271..5441
                     /locus_tag="PK754_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09798.1"
                     /translation="MAEPAQLHAIRGGVSHGGTMSFSTDVLTKIVVIALSITIFGVFV
                     HLYIKSGKSEKN"
     gene            5461..5745
                     /locus_tag="PK754_09590"
     CDS             5461..5745
                     /locus_tag="PK754_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09799.1"
                     /translation="MSISQPESRPLINITELFNSDPEWAAKRDKAIKKLYDGNTQEFN
                     AFMSKLEPMRDWKDVMDAVEAEFMRKKIKQDSKEATGLTDVLFKRYFPSY"
     gene            5923..6165
                     /locus_tag="PK754_09595"
     CDS             5923..6165
                     /locus_tag="PK754_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNB09800.1"
                     /translation="MAYIIAEPCIGVKDTACVKVCPVDCIYDGDGWDQLYIHPDECID
                     CGACEPECPVEAIFAAENTPDKWKSFIQKNADQFKQ"
     gene            6281..>7793
                     /locus_tag="PK754_09600"
     CDS             6281..>7793
                     /locus_tag="PK754_09600"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNB09801.1"
                     /translation="MKKITLPAFIFPCALILLDYVTNKSTVTFNTNRILAYTNGFIGS
                     IAFWTFYQLASAYTLHHYREHKRVRMLFIVGQILFALVFTATISVSLGYYYEFGNLPN
                     KFAVAYLMDEWTDAVSLITGYISMPLMFLLISVFAAIYACHYFVIRHIAQRMHTSTPA
                     SVKKVLAILAIITAASLLGGFFSKKLSATLPMDFGSLQAVAKYAFGDKSGKGYSVPQR
                     IILPENRTARKPSNVLLIINETLRADFIGYQTLANPIPPASRMTDAIASDSLSFVFKR
                     ARSNSSSTRVSLPAILMGMIPTTATSADYHRMPTLWNVSKALGYRTFFVTPQNWDWQN
                     LDVFFLNKQVDQSITAKDIGPPYVNNAGVEDHRILDYTHSIFQEFKKNKNPFFGVIQF
                     NCTHQPYNPGDPHTALSPNSTERYAAAVRYLDSLIAQIYIQLNEAGLKDETLIIVTSD
                     HGENFKHRGLARLNNLYDEVIRIPLWVSYPRKYYQTPEGKARFHAWKQNEYSAV"
BASE COUNT         2174 a         1881 c         1651 g         2087 t
ORIGIN      
        1 ccggatcggc gctgtccgcg atcacattga gcgtggcttt gtgcaatatc gcagccgtta
       61 tgatcatgtt cactcaacta tggcgacgtc ttcgattatt caaacaaatc tataacggca
      121 aatgatccca ctgcatcaaa atcttagaga tattttgatg agaatgcgac gctgaaaaaa
      181 actgcttttc ccaaaacctt gtgagatctg aagtggcaca aaaaacggcc atcgtattac
      241 tgcagcttgg cggaccggat aacttagatg cagtcgaacc gtttctttat aatttatttt
      301 gcgatcccga tattattgat ctgccgggcg cttttctttt tcgtaaaggc ctcgcacgtt
      361 tgatttcctc ccgccgttca ccggccgttc gggaactcta caaaaatatc ggaggccgtt
      421 caccgatctt gccgcaaacc gaagagcagg cacaggcgtt gcaattacga ttggatacat
      481 ccggtcacca atgtaccgta tttatcgcga tgcgctattg gcatcccatg accgatgcga
      541 tcgcccgatc catagcccaa ggcggctatg atcgtattct tttgcttccg ctgtatccgc
      601 attattgcaa agccacgacc ggttccggta tcaacgaatg gaggcgctgt gcaaaaaagt
      661 atcaaattga tcacatccca acccaaactg tcgcaagtta ttttgatcat ccggattata
      721 tacacgctat cgtagataac ataaaaaaag cgctgaccca attgccggaa gatgcgcgcg
      781 cgaagaccca tttggtgttt agcgcacacg gtacgccgat gaaattggtt cgtgacggcg
      841 acccgtattc aatacatatt cgtcgcacct acgaagcggt gttatccgcc ggtaattttg
      901 gtttaccgca tcatttgtgc ttccaaagca aagtcggccc tcagcaatgg cttgaacctt
      961 cgctggtaca aaccatcgct gatctgtcag ctcaaaaagt atcacacatg ctggtcgttc
     1021 ccgtcgcgtt tgtgaccgaa catatcgaaa cgctttccga aatcaatatc gaagtgcgtc
     1081 atgaagcgga acaagcaggc gttcggtatt ttgatatgat gcctgcactc cgcgatcatc
     1141 catcttttac cgcgtgtctc tacgacctca ctctaaaggc attgcacggt gcttggcccg
     1201 tatcgcaatc ataggcggct gatcatatag taatgttcct aagtagatat tgaaaaataa
     1261 ctccttcggg gcgatatacc tttaacaaag aaactttttt aaaaaatacc gtatagatta
     1321 gaaaccataa ccgatacttc gccatgcgtt acgttgatcg ttttcccgat ccgcactccc
     1381 gccgatttga taaagccgaa tggcgcgatg aacatggtac ggccaacgtg atcattcatt
     1441 gccaatcttc aggaattgct tttcccaagc attggggtcc gctctcgctc aaaaccacat
     1501 tatccggtca tgaatactat gtcacacggc atagcacgca ccgcgttacg ccggaacggc
     1561 ttttgatact caatgaagat acgacttacg ccagttatat tcgcgacggt gaaaccgccg
     1621 aatcgtttac gatcaatttc agtgcggatt tcatacgcac cgttttgcgc gatagcctat
     1681 ccgacggtat gcaaaaattg gataatcctt tttccgacga tgcgccttcc gtacaatttc
     1741 cggagtacac gctgccgtat caccatgcta tccgggtccg tctgaaccgt attcgacgat
     1801 gccttaccat gcccgcggat ttgaatgggc gcgtggacga agagctgtct tttttatatg
     1861 aagcgatgtg cacgctttcc gtatcagaaa acaaaaaaat acgcgacctg cctttccaaa
     1921 aagcctcgac tcgcgaggaa atgtaccggc ggcttcagcg tgttcgggat tatatggatg
     1981 cctgttatgc ggaacctata aacctgcacg atctcagtcg cgtagcgtgt ctttccgtgc
     2041 atcatttgat tcgccttttc aaatcgcatt ttttatgtac gccgcatcaa tatctcattc
     2101 gatgccgatt gctcgaagcc aaacgactca ccgaaaccac ttcccttcgt attgaagaca
     2161 tcgcacgccg tgtcggcttt gaagaccgaa gttcattttc acgattgttt cgtcaccatt
     2221 tcgacaaaaa cccctccgat cgcacacaat aaaaaaagca attttcacca ccttgttttg
     2281 acgaaccgct gcgcatatta tagggatgat actattccta aacgaaaggc acatgtatgt
     2341 tgatgcgatc ttttattgtc tcgctttttt ttatatggac taaatccttt gcacaaatgc
     2401 cgatggattc ttccgctttt gatttttggg tgggcgaatg gacggcgact tggtccaacg
     2461 ccgacggcag caccggtaaa ggtaaaaatc gcatcgtcaa aattttagac ggcaaagtga
     2521 ttcaggaaca ttttgaagac gaaagcggat tcaaaggaac aagcatttcc gtatatacgc
     2581 cggctcaaaa aaaatggcga caagcatggg ccgataacca aggcggttat tttgattttg
     2641 aaggcgacat atcggacgga aaaaagattt ttaaaaccaa aatgcacgaa gccaacggca
     2701 aacaatatat gtcacgtatg ctgttttacg atatcaagac ggatgcgttt acgtgggatt
     2761 gggaaaaaac agaggacggc ggcaaaacat gggccttgca atggcgcata caatacgcgc
     2821 gtaaaaaata atggaacgac ggattgaaat ttagttcgcc cccgtcggca catcataata
     2881 tttacttttg atccacaacg cgacgcgaac gaggcttacc aaaacgggca cctcgaccaa
     2941 aggaccgatc acggcggcaa acgctgcacc ggatgagatg ccgaaaaccg ctatggcaac
     3001 ggcgatcgcc agttcaaaat tattgctcgc cgcggtaaac gaaagcgtag ccgacttggc
     3061 atagtccgca ccgctgcgac ggctgagata aaaagaaatc aaaaacatca cgacaaaata
     3121 gatcaccagc ggaacggtga tgcgcaaaac atcgaacgga atcgttacga tatactcacc
     3181 tttgagcgaa aacataacca cgatcgtaaa gagcaacgcg atgaatgtca gcggcgatat
     3241 tttgggaata aacttttcat gataccaaac ccggcctttg gcttttaata aaatcatccg
     3301 cgtaagcatc ccggccaaaa aaggaattcc gagatagata aatacgcttt cggcgatctg
     3361 catgatcgtg atatccaccg caaacgcttg taatccgagc aaggagggaa acaacgtcaa
     3421 aaagagatac gcatacaccg ggaaaaacaa aacctgaaaa atagaattaa aagcaaccag
     3481 acccgccgcg tactccgtat caccatgcgc aagttcattc cacacgataa ccatagcgat
     3541 acagcgtgca agtccgatca tgaccaaacc cgccatatat tccggataat cgcggagaaa
     3601 tacgaccgcc agcaaaaaca taaacacggg gccgattaac cagttttgaa cgagcgacag
     3661 aagcaaaact tttttattcc gaaaaacatc cgcgagttct tcgtatttaa ctttggccag
     3721 cggcggatac atcatcagaa tcaaccctat ggcgatagga atattagtca taccggattg
     3781 gaaccggttc cagaatccga caatggacga aaaaaaataa cctgaaaaaa cgccgatcag
     3841 catggcgata aaaatccaca acgtcagata ccggtctata aatgaaagtt tgctgacttt
     3901 ttccatactc aatcttgcgc tttacacccc ttatcgaagt gatttttgat aaaattcaaa
     3961 aaagtctttt ttaatttcat cacggatgcg gcgaaattcc ttcatcacat gttcttctgc
     4021 cccagaagcc tccgccggat catcaaaccc aatgtgcaat cggtggctca cagttcccgt
     4081 gaaaacaggg cactgttctt gggcattacc gcatacggtg atgacatagt caaacggctt
     4141 atccaaaaaa agatcggtgc ttttgggata gttaccgctg agatcaattc cgatctcgcc
     4201 cattaccttg acggccttcg gatgcacttg agatgaaggc cgggtacctg cggaatacac
     4261 ctcaagcgaa gcatcaaacg atttaagaaa cccttcggcc atttgactgc ggcaggaatt
     4321 cccggtacaa aggattaaga cacgtttttt cataaacctc acctataaat attaattcgc
     4381 taaaaaacga agtattaaga acacgtgatg actttggatg attgtatttt ttttacatcc
     4441 gactgtacct gcgccgtatc gttgagcgcc gtgtgaagta atatacgcaa cgcggttatc
     4501 tccggataca gaggttttgt attcaaactg taatataccc agcggccctt tttctcttcc
     4561 gttatatagg cgtttttttt caaaattttc aaatgctgcg aaacggtgga tggcgccaac
     4621 tttaagacgt cccgtatttc acatacgcac aacgtacgtt gttccagcat tttgagtatg
     4681 cggagcctgt taggatcgga aagcgctttg aatacttcgg cgtaattttg aatagtcata
     4741 ttaattcgtt tattgacgaa ttataataaa caatcgcata ccaagataca attcgttttt
     4801 attgtaaaca caccttaggt ataaatgcgc attatagaag ttatgcttga caatgcgata
     4861 cgaactattc taatttgcgc catgcaacgg atttttaaat cttcattgtt tttcactgtt
     4921 ttcgttttat taagcatggc ttcgcatgcg tattcggcca gccaacaaaa cgaaaccggt
     4981 ttcccgcata aaacgacaca agagccgacc tagtcatcat tggaccagcg tgacggttta
     5041 ttttttcttt catcatcccg cgaagaaatc gcatcccaat taaaagaaac tcgtcataaa
     5101 gcgtggattc tgccggcatt gtccgtttct ttcattacgt accgcaaata cctcgatttt
     5161 tttcaactct cactgactcg caattttctt ctcggatata cgacaggtct tcgcgccccg
     5221 ccgcgttgct gacatctccc cctctataaa tcatttatcg gccacccggt gtggctgaac
     5281 cggcacagct tcatgccatc cgtggtggtg tttctcacgg aggtactatg agtttttcaa
     5341 ccgatgtgct caccaagatc gttgtgatcg ccctatcgat cacgatcttc ggggtgtttg
     5401 tacatcttta tatcaagtcc ggaaaatctg aaaaaaactg acatcaaacg gagtatatct
     5461 atgtcaatct ctcaacccga atcgcgccct ttgatcaaca ttacggaatt attcaattcc
     5521 gatccggaat gggccgcgaa gcgtgataaa gctattaaga aactgtacga cggtaatacg
     5581 caggaattca acgcgtttat gtccaaactg gagcccatgc gcgactggaa agacgtgatg
     5641 gacgctgttg aagccgagtt tatgcgtaaa aagatcaaac aagacagcaa agaagcaacc
     5701 ggtcttacgg atgtgctttt caaacggtac ttcccctcgt attagtccgt ctggatatat
     5761 tcatttctta ctccgagctg cgcgaataat ttgcgcagct cttttgtttt atataacctc
     5821 tctttgccga aaaaatcttg actattcacg gcttaatttt tatgttgaac cacgataacg
     5881 acggatttgt tctcactata aaaaattttc agaggagtag atatggctta tattattgca
     5941 gaaccgtgca tcggtgttaa agatacagcc tgcgtcaagg tttgcccggt ggactgcatt
     6001 tatgacggcg acggttggga tcagttgtac attcatccgg atgaatgcat tgactgtggc
     6061 gcatgtgaac ccgaatgtcc tgtcgaagcg attttcgccg ccgaaaacac gcccgataaa
     6121 tggaaaagtt ttattcagaa gaacgcggat caattcaaac aatagatccc ttcttgatta
     6181 cacacgaaaa ggcttggact tgttccaagc ctttttattt tttactctcg tcgttgccgt
     6241 agaaccgcac ctgccgtgca aaaaaaatgg ggaaatcaaa atgaaaaaaa tcacgttacc
     6301 ggcgtttatc tttccttgcg ctttgattct gctcgattat gtaaccaata aatcaaccgt
     6361 tacttttaat accaaccgta tcctggcgta taccaacgga tttatcggtt ctattgcgtt
     6421 ttggactttt tatcaattag cttcggcgta cacgctgcat cattatcgcg aacacaaacg
     6481 ggttcgcatg cttttcattg tcgggcaaat tctttttgcc ttggtattta ccgctacgat
     6541 ttcggtaagc ctcggatatt attatgagtt tggtaacttg cccaataagt ttgccgtggc
     6601 gtatctgatg gatgaatgga ctgacgcggt gagtcttatt accggttata tttctatgcc
     6661 gctgatgttt cttctgattt ccgtattcgc agcaatatat gcctgccact attttgtgat
     6721 acgtcatatt gcacaacgta tgcacacgag taccccggct tcggtgaaga aagtcttagc
     6781 gattttggcc atcatcaccg cggcatcgtt gctcggaggt tttttttcca aaaaactgtc
     6841 cgctacgtta ccgatggact tcgggagttt acaagccgtt gcaaaatatg cgttcggcga
     6901 taagtcggga aaaggctatt ccgttcccca gcgaattatt ttaccggaaa atcggaccgc
     6961 ccgaaaacca tccaatgtat tgttgatcat caacgaaacg ttgcgcgccg attttatcgg
     7021 ctatcagaca ttggccaatc cgattcctcc ggcttcacgc atgaccgacg caatcgcttc
     7081 cgacagcttg tctttcgtat tcaaacgcgc acgcagcaat tcgagttcta cccgtgtcag
     7141 tttgcccgct attctcatgg gcatgattcc cacaaccgcc acttccgccg attaccatcg
     7201 catgccgacg ctttggaatg tgtctaaagc gctgggttac cgcacctttt ttgtcacgcc
     7261 gcaaaattgg gattggcaaa atctagacgt attttttctc aataagcagg tggatcaaag
     7321 tataacggct aaagatatcg gcccgcccta cgtcaataat gcgggtgtgg aggatcaccg
     7381 catattggat tacactcatt ctatttttca ggaatttaag aaaaacaaaa atccgttttt
     7441 tggcgttatt cagtttaact gcacacacca gccgtataat ccgggcgacc ctcatacggc
     7501 gttatcacct aactcaaccg agcggtatgc cgctgcggta cgatatttgg acagtttgat
     7561 tgcacagata tacattcaac tcaatgaagc cgggctgaaa gacgaaacgc tgatcatcgt
     7621 gacaagcgat catggtgaaa attttaaaca tcgcggtctt gctcgattga ataatcttta
     7681 cgacgaagtc atacgcattc cattatgggt aagttatccg cgcaaatatt atcaaactcc
     7741 cgagggcaag gcgcgatttc acgcctggaa acaaaatgaa tatagcgctg ttc
//
LOCUS       DAOQNQ010000143         7777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3511_length_7777_cov_5.870888, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000143 DAOQNQ010000000
VERSION     DAOQNQ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3511_length_7777_cov_5.870888"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PK754_09605"
     CDS             complement(<1..499)
                     /locus_tag="PK754_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNB09802.1"
                     /translation="MKQSVFMSILITTAFVVSYLVFEFIFGAEGDKYGLHYIYAGGPL
                     VVILMVLSIMVVTFIFERIFSLRKANGKDSLPFFLKKIQKSLMDGNVETSIEVCDHQK
                     GSCANIIKMGLEAFQRGSKMGMNMEKRVTETKRAIEEATSLEVPLLERNLIALSTIAS
                     ISTMVG"
     gene            complement(611..946)
                     /locus_tag="PK754_09610"
     CDS             complement(611..946)
                     /locus_tag="PK754_09610"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNB09803.1"
                     /translation="MKVKTDPSGVVVIAPKGRLTGGSETDELKQEIERVVAEGITKCV
                     IELSETIYLNSTALGLLVSAHKKFIDRGGKLKLSGVNKNIDNILVITKLSLVFEVFDT
                     EAEALASFK"
     gene            complement(1016..2647)
                     /locus_tag="PK754_09615"
     CDS             complement(1016..2647)
                     /locus_tag="PK754_09615"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB09804.1"
                     /translation="MRSFLKKTLVITLMLISANQVVVAQGPQIKQALLLINKGQNKEA
                     VEYLLQIVNEYPKTAELRYLLAMALDKNGQTDMAIEEAKSVVTMKPKYKDNRRLLMDL
                     YIKKENFKNAMEECQFLLKESKKDIGLKLNLCEIYLGLENYKDASTELAKLEVEKPND
                     NLVLTLLGDAYAKQRVNSLAIQYYNKALGVNPNAVNTKLKLAKLYFRETQYNEALKEY
                     LDVVRMDTTNAEALLRIGIIYNAAGKSDVNQYGRAIGFLQRYVGIAPNDYAGYFYLGK
                     SWHALRSWQNAVTNFESAIKLDDSARVKEESTALLSESYRGLGVNYLKDKNYDAMTPA
                     IKKSIELNPASDSIAFEIGYSFYGEKNYATAASWFDISAQSKTASEPKIANSLFLSGV
                     SRYNAATTKADSMAALAPIEKSLALRPTLNNYLNFAQYNESVDQPAKARAAYEKAVEM
                     DSNSVTAQLKLGILLFNKFKNDTEPAIKAFKKVGQLDEKGSYKMSHYFLGLCYLNAKK
                     KPDAREAFKKYLEVDPTGNFSKQAKTYVTQLAGAR"
     gene            complement(2787..3734)
                     /locus_tag="PK754_09620"
     CDS             complement(2787..3734)
                     /locus_tag="PK754_09620"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNB09805.1"
                     /translation="MKNKIHGFVVILLCGLFWIGCKGNERATTPTTGALPLICDDAVS
                     NVIQIQKNEFEKRYTQAKIDMQLAPASAAIVKLINNEVECIISSRELDSTETDFLIRH
                     EIKVFSQKFALDGIAIIVNSENPIEEMSLASLRELFSGDATYWSDITDSVRFPYHLNR
                     IQIVSDGRRSGNYRVLHEMVLDKNPLSPNAVVLSGDSANSVVPKILDFVASTPGAIGY
                     VSTAWVGKTSADWESRSGKLKVIRVSGEDFHKAVEPIQGYVYRGDYPLRRVLYIIHRQ
                     PYIGLAAGFTAFLTGNDGQKICLANNLVPGMNPIRLKHE"
     gene            complement(3724..3957)
                     /locus_tag="PK754_09625"
     CDS             complement(3724..3957)
                     /locus_tag="PK754_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09806.1"
                     /translation="MIGEWDINRKIRFGVALLFVATGIWVMIGNVMPDYINDRYHYVM
                     GFILIFYGVFRFVHYDYRKNIKRTDEISEENEE"
     gene            complement(3959..5542)
                     /locus_tag="PK754_09630"
     CDS             complement(3959..5542)
                     /locus_tag="PK754_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNB09807.1"
                     /translation="MSDAKKSKTVLTDAGIEVPLYLKEYPQDYSTKLGDPGQFPYTRG
                     VQNTMYRGRLWTMRQYAGFGNAEESNKRYKMLLAQGTMGLSVAFDLPTQIGYDCDHAL
                     AEGEVGKVGVSISSLEDMEVLLDGIPLDQISTSMTINATAAILLCLYVAVAKKRGVDI
                     RQLSGTIQNDVLKEYIARGTYIYPPKHSMRIITDIFRYCTSELPKWNMISISGYHIRE
                     AGSTAVQELAFTFANAIAYVEAAVASGQDVDQFASRLSFFFNAHNNFFEEIGKFRAAR
                     RIWAHIIKERFGANDPKSMMLRFHTQTGGSTLTAQQPYNNIPRVTLQALSAVLGGTQS
                     LHTNSFDEALALPTEDSARIALRTQQVIAYESGVAQSVDPLAGSYLVEQMTDEIETRV
                     MRYLKDIDAMGGSIRAIESGYFQSEIAEAAYRYQKAIETKAQIIVGVNDFKVENDSKP
                     DILQIDTSIEKNQKERIQKLRARRDNAKVQSGLEKLAQACRGTDNILPCILESVEAYA
                     TLGEIADTMRNVFGEYKAA"
     gene            complement(5539..6186)
                     /locus_tag="PK754_09635"
     CDS             complement(5539..6186)
                     /locus_tag="PK754_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNB09808.1"
                     /translation="MQEPWRLLRIMAEFVDGFEKMERVGPAVSIFGSARTKPGMANYK
                     LAEELARLIVKSGFAVITGGGPGLMEAANKGAKEAGGKSVGLSIQLPFETKGNDFLTM
                     EVNFNYFFVRKVMFVKYARGFIALPGGLGTMDELFEMLTLIQTGKVDPFPIVLIGKTY
                     WSGLLDWMKASMLKERYINPEDLELFHVTDSAKEAVRIIQDFYKKNKKRGKIRII"
     gene            complement(6263..7261)
                     /locus_tag="PK754_09640"
     CDS             complement(6263..7261)
                     /locus_tag="PK754_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB09809.1"
                     /translation="MNVALSQTPTVLIVDDSEDMILLLTEVLEDAEYRVVTARNGHEA
                     LDMVVQANPDLILLDAIMPNMNGYEVTQILKTQDKTRLIPIIMLTGLSDLNDKLKGIE
                     LGVDDFLVKPFNHLELVTRVRSLIRVKQYTDELENAETVIFSLALAVEAKDEYTEGHC
                     NRLSFYGARLAEAIGLSTEQIKAVRRGGILHDIGKIAIHDNLLLKPGPLTPDEFEVMK
                     QHTVIGERICKPLKSLNGVLPIIRSHQERWDGSGYPDGLKGEEIPIIARVIMTVDLYD
                     ALTTDRPYRRALSNEKAFEVMREETAMGLWDAMLMEKFIDMMQKNEFGTPTGRSAL"
     gene            complement(7274..7726)
                     /gene="queF"
                     /locus_tag="PK754_09645"
     CDS             complement(7274..7726)
                     /gene="queF"
                     /locus_tag="PK754_09645"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNB09810.1"
                     /translation="MKKKIHHKHVTDAKAFVQKPDFKFKFEGYEAIRPELIETFAYEN
                     PGQPQAIVVSTEEFTAVCPWSGLPDFARIVVTYVPRQKIIELRSYKYYLYSYRNVGIF
                     QEHLTPRLLQDLVAACEPESMTVKTDYNIRGGIHTVCEASYRRNTKRR"
BASE COUNT         2092 a         1834 c         1595 g         2256 t
ORIGIN      
        1 atcccaccat ggtggaaatc gaagcgatcg ttgaaagcgc gatcaggtta cgttcgagca
       61 acggtacttc taaagacgtg gcttcttcga tagcacgttt tgtttcggtc acgcgttttt
      121 ccatgttcat acccattttt gaaccgcgtt gaaacgcttc caaacccatt ttaataatgt
      181 ttgcgcagga acctttttga tgatcgcaaa cctcaatcga agtttccacg ttgccatcca
      241 tcaggctttt ctgaattttc ttgaggaaaa aaggaaggct atctttgccg ttagctttac
      301 gcaacgaaaa aatacgttca aaaataaatg tcacaaccat gatggacaga accatcaaaa
      361 taactacgag gggaccgccc gcgtaaatat agtgaaggcc atatttgtca ccttcggcac
      421 cgaaaatgaa ctcgaacacc agataagaca cgacaaatgc cgtggtgatc aaaatagaca
      481 tgaatactga ctgcttcata aggaaggtcc tcctgaaaaa taggatttat ttaatgtatg
      541 actaagattt gccttaaaca ggttcgggaa agatacgagt ctatcccgaa gcatacaagt
      601 tatttttatg ttacttaaaa ctcgcaagcg cttcggcttc ggtatcaaac acttcaaaca
      661 ccagtgatag tttggtgatg acgagtatat tatcaatatt tttattaacg ccggataatt
      721 tcaattttcc gccacggtca ataaattttt tatgcgcgga taccaaaagc cctaaagccg
      781 tactattcag ataaatcgtt tcggataatt cgattacgca cttggtaatc ccttccgcta
      841 caacgcgttc gatttcctgt tttagttcgt ccgtctcgga acctccggtc agacggcctt
      901 tgggggcgat caccaccacg ccggacggat cagttttgac tttcatcgca atctcctttt
      961 ataatatttc aacttttcaa tagtaccaat caacacaatg cttgggcgtt aaaaactagc
     1021 gtgcgccggc aagctgtgta acatatgttt tagcttgttt agaaaaatta cccgtcggat
     1081 ctacttctaa atattttttg aatgcttcac gtgcatccgg ttttttcttc gcattgagat
     1141 aacacaaacc taagaaataa tgcgacattt tataggaacc tttttcatca agctggccga
     1201 cctttttaaa tgccttgatc gcaggttccg tatcgttttt aaatttatta aacaacaaaa
     1261 ttccaagctt gagttgtgcc gttacactgt tgctgtccat ttcgacggct ttttcgtaag
     1321 ctgcacgcgc tttagcgggc tgatctaccg attcgttgta ctgtgcaaag ttaagataat
     1381 tattcaatgt gggacggagc gctaatgatt tctcaatcgg cgccaatgca gccatgctgt
     1441 cggctttggt cgttgccgca ttataacgcg atacgcctga taagaatagg ctgttggcaa
     1501 ttttgggctc gctcgcagtt ttgctttgcg cagaaatatc aaaccatgat gcggccgtcg
     1561 cataattttt ttcgccgtaa aaactatatc cgatttcaaa cgcgatcgaa tcactggccg
     1621 gattaagttc gattgacttt ttgatcgccg gggtcattgc atcatagttt ttgtctttga
     1681 gataattcac accgagaccg cggtaggatt ccgataaaag agcagtcgat tcttccttaa
     1741 ctcgagcgga atcgtccaat ttgatcgctg attcgaaatt ggtaacagca ttttgccagc
     1801 tacgaagggc gtgccagctt ttaccgagat aaaagtatcc agcataatca ttgggcgcta
     1861 taccgacata tcgttggaga aaaccgatag cccggccata ttggttgaca tcggattttc
     1921 cggcggcgtt atatataata ccgatacgca aaagtgcttc ggcattggtc gtgtccatac
     1981 gcacaacatc gagatattct ttgagcgctt cattgtactg cgtttcacga aaatacaact
     2041 tagccagttt gagtttcgtg tttactgcat taggattaac gcctagggct ttattataat
     2101 attgtatagc cagtgaattg acacgttgct tagcatacgc atcacctaat aacgtcagta
     2161 ccaaattatc attgggtttt tctacttcga gcttagccaa ctccgtgctg gcatctttat
     2221 aattttcaag accgaggtat atttcgcaca gattaagctt gaggccgata tcttttttgg
     2281 attccttaag aaggaactgg cactcctcca tcgcattttt gaagttttct tttttaatat
     2341 aaagatccat caaaagccga cggttatctt tatatttagg cttcatcgtc acgacgcttt
     2401 tggcttcttc gatagccata tccgtttgac cgtttttatc taatgccatg gccaatagat
     2461 agcgcaattc tgcggtttta ggatattcgt ttactatctg aagcagatat tccacagctt
     2521 ctttattttg ccctttattg atcagtaata atgcttgttt gatttgtgga ccttgtgcaa
     2581 caacaacttg gttagctgaa attaacatca aagttataac taacgttttc ttcaagaaac
     2641 tacgcatact aaggtacttc ctctttgtta aaataagatc ggatctaaca ataaattata
     2701 gacaagatat agattcgtca aaaaccggat tgggaccaaa aatgacggaa aaatctatgc
     2761 gattttagaa aaaaaacaag ctttttttac tcatgcttaa gccggatggg attcatcccc
     2821 ggaacaaggt tattggccaa acatattttt tgtccgtcat tgcctgtcaa aaatgccgta
     2881 aaaccggccg ccaacccgat atacggctga cggtgaataa tatataatac ccgtcgcaac
     2941 ggataatcgc cgcgataaac atacccttgt atgggctcta ctgctttgtg aaagtcttca
     3001 cccgacacac gaataacttt taatttgccg gaacgcgatt cccaatccgc gctggtttta
     3061 cccacccaag ccgtgctcac atatcctatg gcgcccggcg ttgatgctac aaaatccaaa
     3121 attttgggta caacactatt ggccgagtcg ccggacaaaa cgacagcatt gggtgacaac
     3181 ggatttttgt ccagtaccat ttcgtgcaaa actctataat taccggagcg acgaccatcg
     3241 ctgacgattt gaattcggtt gagatgatac ggaaatcgca ccgagtctgt gatatccgac
     3301 cagtacgtcg catctccgga aaaaagttct cgcagcgaag caaggctcat ttcttcgatg
     3361 ggattttccg aattgacaat aatggctata ccatcgagcg caaacttctg agaaaaaacc
     3421 ttaatttcgt gacgaattag aaaatccgtt tccgtcgaat ccaattcacg cgaactaatg
     3481 atacactcca cttcgttatt gatcagcttg acgatcgccg ccgaggccgg cgctagctgc
     3541 atatcaattt tagcttgtgt gtaccgtttt tcaaattcat ttttttgaat ctgaataaca
     3601 ttagaaacag catcatcgca gatcaaaggt aaagcacctg tcgtaggtgt tgttgctctt
     3661 tcgttaccct tacaaccgat ccaaaataaa ccgcataata agataacgac aaatccgtgt
     3721 attttattct tcattttctt ccgaaatttc atcggtacgt ttgatgtttt ttctataatc
     3781 ataatgcacg aagcgaaata cgccataaaa tataagtata aagcccatta cgtaatgata
     3841 acgatcattg atataatccg gcataacatt accgatcatc acccaaatac cggttgcgac
     3901 aaaaagcaag gctacgccaa aacgaatttt gcgatttata tcccattccc cgatcatttc
     3961 aagcggcctt atattctcca aaaacgttgc gcatcgtatc ggcgatttcc cccaaagtgg
     4021 cataggcttc taccgattct aatatacacg gcaggatatt gtccgtaccc cggcaagcct
     4081 gagctaattt ttctaacccg gattgaactt tagcattatc acgtcgcgca cgaagttttt
     4141 gaatacgttc tttttgattt ttttcgattg atgtatcaat ctgaagtatg tccggcttcg
     4201 aatcgttttc gactttgaaa tcattgacgc cgacgataat ctgcgccttg gtttcgatcg
     4261 ccttttgata acggtatgcg gcttcagcga tttcactttg aaaataaccg gactcaatcg
     4321 cacgaatgga accgcccatc gcatcaatgt ctttcagata gcgcatgact cgcgtttcga
     4381 tctcatccgt catttgttcg accagataac ttccggcaag cggatcaact gactgtgcaa
     4441 cgccgctttc atacgcaatc acctgctgtg tgcgaagggc aattcgcgcc gaatcctctg
     4501 tcggtaatgc cagcgcttca tcaaacgaat tagtatgtaa actttgcgta cctcccaaaa
     4561 cagccgataa cgcttgtaac gtcacgcgtg gtatgttatt gtatggctgc tgggctgtca
     4621 gcgtagaacc gccggtctgc gtgtggaaac gcagcatcat gcttttgggg tcattcgcac
     4681 caaatcgttc tttgatgata tgtgcccaaa tccttcgtgc cgcacgaaac ttgccgatct
     4741 cttcaaaaaa attattatgt gcattaaaaa agaaactcag tcgcgaggca aactgatcca
     4801 catcctgacc ggaagcaacg gccgcttcaa cataagcgat ggcattggca aatgtaaatg
     4861 ccaactcctg cacagccgtg gagccggctt cgcggatatg atacccggat atcgaaatca
     4921 tattccattt gggcaactcg ctcgtacaat agcggaaaat atcagttata atccgcatgg
     4981 aatgtttggg tggataaatg tacgtaccgc gtgcaatata ttcttttaga acatcgtttt
     5041 gtatcgtacc ggaaagctgg cgaatatcta cgccgcgttt ttttgctacg gcaacgtaca
     5101 ggcataataa aatcgccgct gtcgcattga tcgtcatgga tgtgctgatc tgatccagcg
     5161 gaatgccatc caaaagcact tccatgtctt cgagagatga aatcgaaaca ccgaccttgc
     5221 ctacttcgcc ttcagccagt gcgtggtcgc aatcgtaacc gatctgtgtg ggcaaatcaa
     5281 atgctacgga caaccccatc gtaccttgcg ccaaaagcat tttgtagcgt ttgttggatt
     5341 cttctgcgtt tccaaatccg gcatactgac gcatggtcca cagacggccg cgatacatcg
     5401 tattctgaac accgcgtgta tagggaaact gtcccggatc gcccagcttc gtcgaataat
     5461 cctgcgggta ttccttgaga tacaagggca cttcgatacc cgcatcggtc agcacggttt
     5521 tacttttttt tgcgtcgctc atattattct gatttttccg cgttttttat ttttcttata
     5581 aaaatcctgt atgatgcgca ccgcttcttt agccgaatcg gttacatgaa acagttccaa
     5641 atcttcgggg ttgatgtatc gttctttgag catggatgct ttcatccaat caagcaaacc
     5701 gctccagtag gttttaccga taagcacgat cggaaaggga tccactttac cggtctgtat
     5761 taacgtcaac atttcaaata attcgtccat cgtacccaga ccgccgggca aagcaataaa
     5821 accgcgggcg tacttgacga acatgacctt gcgcacaaag aaataattga agtttacttc
     5881 catcgtcaga aaatcattgc ctttggtttc aaagggcagt tggattgaga gcccaacgga
     5941 cttcccgcct gcttctttgg cgcctttatt agccgcttcc ataagccctg ggccgcctcc
     6001 ggtgatcacg gcaaaacctg atttgacgat taggcgcgct aattcttctg ccaatttata
     6061 attagccatg cccggttttg tgcgggcgga accgaatatc gaaacagccg gtcccacgcg
     6121 ttccattttt tcaaagccat ccacaaactc ggccatgata cgcaaaaggc gccacggctc
     6181 ttgcatgcga taggacttat ctacggtttc taaattatca tgctgattgc ggtgattctt
     6241 aggcaactgt tttctcctgc atttataagg cgctgcggcc ggtcggtgta ccgaattcgt
     6301 tcttctgcat catatcaata aatttttcca tcaacatggc atcccataaa cccatcgctg
     6361 tttcttcgcg cataacctca aaggcttttt cattggataa agcccgacgg taaggacggt
     6421 ctgtagtcaa tgcgtcgtat aaatccacgg tcatgatcac acgggcgatg ataggtattt
     6481 cttccccttt aagcccgtcc ggatatccgc ttccgtccca tcgttcctga tgactgcgaa
     6541 taataggtag tacgccgttg agcgatttga gaggcttaca aattcgctcc ccgatcaccg
     6601 tatgttgctt catcacttca aattcgtccg gcgtcaacgg accgggctta agaagtaaat
     6661 tatcatgaat ggctatctta ccgatatcat gcaaaatgcc gccgcgacga acggccttga
     6721 tctgctcggt agataaacca atcgcttccg caagcctggc gccataaaac gataatcggt
     6781 tacagtgccc ttcggtatat tcgtctttgg cttcgaccgc cagcgccaaa ctaaaaatta
     6841 cggtctcggc gttttcgagt tcgtccgtat attgttttac ccggatcaag gaacgaacgc
     6901 gcgtcaccag ttctaaatgg ttaaacggtt tgacaagaaa atcatcaaca ccgagttcga
     6961 tccctttgag tttgtcgtta aggtcagaca aaccggttag catgataatc ggtatgaggc
     7021 gagttttgtc ttgtgttttg aggatctgtg tgacttcata accgttcata ttaggcatga
     7081 tcgcatcaag caatatcaaa tccggattgg cctgtacgac catatccaac gcttcgtgtc
     7141 cattgcgtgc agttacgacc cggtattcgg catcttccaa aacctccgtc agcagaagga
     7201 tcatatcctc gctgtcatcg acgatcaaaa cagtaggtgt ttgcgacaaa gcaacattca
     7261 taagtacaac cttttaacgg cgtttggtgt tccgacgata tgaggcttca cataccgtgt
     7321 gaatgcctcc ccgaatatta taatccgttt taactgtcat gctttcaggt tcgcatgcgg
     7381 ccacaagatc ctggagtaat cgcggtgtca aatgttcctg aaatataccc acattgcgat
     7441 aggaatacag gtaatatttg taggaacgca attcgatgat tttttggcgc ggcacatacg
     7501 tcacaacgat ccgcgcaaaa tccggaagac ccgaccaggg acatacggcc gtaaattctt
     7561 ccgttgaaac cacgatcgcc tgaggttggc cgggattttc atacgcaaat gtttctatca
     7621 attcaggacg aatcgcctca taaccttcaa acttaaattt gaaatcgggc ttttgtacaa
     7681 acgctttggc atcggtaacg tgtttatgat gtatcttttt tttcactgct gcctcttcaa
     7741 gcaagtatag tttttaaaac atcggatgta ttagaaa
//
LOCUS       DAOQNQ010000144         7752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3528_length_7752_cov_5.261401, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000144 DAOQNQ010000000
VERSION     DAOQNQ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7752
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3528_length_7752_cov_5.261401"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="PK754_09650"
     CDS             complement(<1..1116)
                     /locus_tag="PK754_09650"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HNB09811.1"
                     /translation="MEYISVFDIFKIGVGPSSSHTMGPWRAAQRFLRELDDTNRLCDV
                     TRVQAHLYGSLAKTGKGHGTDMAILLGLSGEDPVTTEVESIPSRIAKIRSSHNLGLLG
                     QHPIPFDTDHDIVFHYDASLPFHANGLRFVAHFKDGALFEEIYYSVGGGFVVRENDTE
                     HDPSGMQTVPFPINNGDELLQRCREKRWPISKIVFENEKVFRREDAIHSALLRIWKVM
                     TECVFRGCHTEGDLPGGLKVQRRASAMNRRLLNNQDYPTQEDWISAVQSVPKSFQDML
                     NWVSCFALAVNEENASYGRVVTAPTNGAAGVIPAVLHYYRYLCPNVHDQSVIRFLLTA
                     GELGSIFKKGSTISAAMGGCQAEIGVSSAMAAGALTEC"
     gene            complement(1135..2085)
                     /locus_tag="PK754_09655"
     CDS             complement(1135..2085)
                     /locus_tag="PK754_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HNB09812.1"
                     /translation="MNRTDRLIAIVLLLHSRKIIRAKDIAEHFDITLRTVYRDMKALN
                     EAGVPIAAEAGEGYSLVEGYHLPPVMFTHEEAAALLVGSELTRSLTDASLQTHITSAL
                     HKIQAVLPQDRKNYLDNLREALHIVTYPSRFKEGFRDDVLKTIQDAIVHRRILTIEYI
                     TNRDDRQSKRNVEPLGLVYYSNFWHLIAYCRLRKDYRNFRTDRITAIQVKDESFASRD
                     DFSLTQYLSQSRPVENLIQVRVKFTYEVVSYVRDRNYFGIVSEEKTEGGVIFTFLTPE
                     LKYLSGWLLGYGEHAEILEPKALKQIMYDEAVKLTQMYKP"
     gene            2178..2330
                     /locus_tag="PK754_09660"
     CDS             2178..2330
                     /locus_tag="PK754_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09813.1"
                     /translation="MKGMKLSHLLLCIFLIGEGLISILGLKFDGIHFVMGGIALAAGV
                     LPFLDK"
     gene            complement(2568..3329)
                     /locus_tag="PK754_09665"
     CDS             complement(2568..3329)
                     /locus_tag="PK754_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09814.1"
                     /translation="MHNLHVGIAFAQIHANMKLNQIHTLKPNLEGKMKKILLTVALIL
                     KLGNISSLSAQDNQARIESKIDTLMSYQKMIYSEVKKEDPLATRGAGFEINPARLLVM
                     SDDRLSISGTYSMFAIDRRAEIAFPFLYEQGDKRHGSTRLINLDAQYRRFLGDRQGGL
                     YFSGGLRFTSLKGYEGSEWNWDEEISTNKVTENKLGVLCGVGYRYFTKSGFFWGTSLS
                     VGRYFKYTDKSIQDNVLDDGKIIVDFEILKFGFAF"
     gene            3703..4599
                     /locus_tag="PK754_09670"
     CDS             3703..4599
                     /locus_tag="PK754_09670"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNB09815.1"
                     /translation="MEYENKLDISFETKPQLPGIGTSFLILILGILTGFVFMGIFVVI
                     GKFIVPAESVFVLNKIGLVLGTMLFVLPALIYARIHKLDVVLLFRMRRVNLSVMIISV
                     VTALGLVVVTDTLDRWMAPAINAWLDSTIGVLSPELMSDRILEQLAEEMKIHSFTEGF
                     LLILGAVIAAGICEEILLRGLLQRSLEAHYPVLISVAISSLVFALIHFNPWGGIQIFI
                     IAIVLGAVAWRTQSILPTIVFHSVNNFTVLIFNNLEPSALLWYGDEHHVFPWVSMGGF
                     ICTLLGLWRIWILTSIEKEETK"
     gene            4596..5201
                     /locus_tag="PK754_09675"
     CDS             4596..5201
                     /locus_tag="PK754_09675"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HNB09816.1"
                     /translation="MNRRVTVAITGASGVIYAVRLLRYFLTHAYEVDFIISDAGKINF
                     KVEMDWDLDKKGIVEFLKNKFGHDIDKGKLREWPVQAIAAPMASGSMRRLGMVVVPCS
                     MKTLSGIAHGASTNLIERAADVALKENFPLIIVPRETPMNSIQIENMLTLSKAGARIV
                     PAMPAFYQKPQSIDDIADFIVARILHMLGIDDHGLFQPWGG"
     gene            5206..7479
                     /locus_tag="PK754_09680"
     CDS             5206..7479
                     /locus_tag="PK754_09680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNB09817.1"
                     /translation="MQRKRREDDDMPESSDDHAKEIRIPVKPGHEPKKSALNKTNHSS
                     AWILALKLFSAVVFLLLTVVIFFAYYFRDDLPSIEQLEVYEPKLSTKVYSADGKVIKE
                     FFRERRTRIPIQEIPKSITEALLATEDHRFYDHWGMDIIRFVKSAMVNTLTLSSSEGF
                     SSLTQQLARNLYLSKEKTLSRKFKEVLTAIQIEKTYSKNEIMEMYLTHMYFGHDAYGI
                     HAAAKIFFHKDVRELSLAESAYLVGHLQAPSFYMNNIQAANTRKNTVLYRMREVGYIG
                     ETEFSAARQEKIQTFPRRDEDVIGYAPYFAENVRRELEYLSDSLHFNVYEDGLNVFTT
                     LDTRAQAAAEKAYEENVKERFGPLDKFGAEYLAANAQQYLPKYMRREGVSPERIERLL
                     ASPRAVDSLSRFYGTVQSGFVAVDPSNGHVLALIGGRDFKKYKLNHVTQIARQPGSTF
                     KPFLYTAAIDNGYSPNFKLLNQDVVIITEDGKRWTPQNYDGGRGGPTTLRDALATSLN
                     LVAIRLMQEVVPPATVITYAQKMGIQTTLQPVDALALGVFDVVPMQIVSAYGVFANKG
                     IRVDPVFITRITDKDGNLLYQHIPKYKEVLSAETAYIMAEMMKSSIDSERGSGRLARR
                     FGFKRPAGVKTGTTQKWSDGWYIGYTPQIVAGVWVGYDTYELNLSARNPGEVIAAPMW
                     GRFMLDAHEAMGLPEADFEMPPGVVRLEICEDSGQLAITACPKKYKEVFNAKFQPTDP
                     CAVHKTTVGKKKKGRGY"
     gene            7485..>7752
                     /locus_tag="PK754_09685"
     CDS             7485..>7752
                     /locus_tag="PK754_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNB09818.1"
                     /translation="MPVNEVQLNAYAKINLGLRIHEKRGDGYHNIETVFKIISLHDRV
                     AIRLNDRGGARLTCGLAGVPTDESNIMMKAVRSLEKKSGRPIHTD"
BASE COUNT         2132 a         1625 c         1813 g         2182 t
ORIGIN      
        1 acattcggtg agggcgcccg ccgccatggc ggaagaaaca ccgatttccg cctgacaacc
       61 gcccatcgcg gcagaaatcg tggaaccttt tttgaatata cttccgagtt cgcctgcggt
      121 gagcaaaaaa cgtatgacac tttgatcgtg gacattaggg catagataac ggtagtaatg
      181 taaaacagcc ggtattacgc ccgccgcgcc attcgtggga gccgtgacga cgcggccata
      241 actcgcgttt tcttcattca cggccagtgc aaaacaactg acccagttga gcatatcctg
      301 aaacgatttc ggaaccgatt gtacggcaga aatccagtct tcctgagtgg gataatcctg
      361 attattgagc aatcggcgat tcatcgcgct ggctcggcgc tgaactttga gtccgcccgg
      421 aagatcgcct tcggtgtggc aaccccggaa aacacattcg gtcatgactt tccaaatacg
      481 caaaagcgcg ctgtgtatgg catcttcgcg gcgaaaaacc ttttcatttt caaaaacaat
      541 tttagaaatc ggccaacgtt tttcccggca cctctgcaaa agttcgtcgc cgttattgat
      601 cggaaaagga acggtttgca ttccggaagg atcgtgttca gtatcatttt cgcgtacgac
      661 aaatccgccg ccgacggaat agtatatttc ttcaaataat gctccatcct taaaatgagc
      721 gacaaatcgc aacccgttgg catgaaaggg aagtgaagcg tcgtaatgga aaacgatgtc
      781 atgatccgta tcaaaaggta taggatgctg gcccaataaa cctagattgt gcgatgatcg
      841 tattttagca atgcgcgacg ggatggattc gacttctgtg gtaacgggat cttcgccgct
      901 aaggccaagc aatatagcca tatccgtgcc gtgacccttg ccggttttag ccaaagaacc
      961 ataaagatga gcctgcacac gggtcacatc gcaaagtcga ttggtatcat ccaattcacg
     1021 caaaaaacgc tgcgctgcgc gccaagggcc catcgtatgc gaactggaag gacctacgcc
     1081 gattttgaaa atatcaaata cgctgatgta ttccatggag tgtagttctt aattttaagg
     1141 tttatacatt tgagttaatt tgaccgcttc atcatacatg atttgtttca gtgctttcgg
     1201 ctcgagtatt tcggcgtgtt caccataacc caaaagccat ccggataaat attttagctc
     1261 cggtgtgaga aatgtaaaaa taacaccgcc ttcggttttt tcttccgaca cgataccgaa
     1321 ataatttcga tcccgcacgt atgacactac ttcgtatgta aatttgaccc gaacctggat
     1381 caagttttca acaggacggc tttgcgaaag gtactgagtt aaagaaaaat catcgcgtga
     1441 agcaaacgat tcgtctttga cttggattgc cgtaatccgg tcggtgcgga aattgcgata
     1501 atctttgcgc aagcggcaat aggcgatcag atgccaaaaa ttagaataat ataccaaccc
     1561 taaaggctcg acattacgct tggactgacg gtcatcacga ttggtgatgt attcgatcgt
     1621 taagatacgt cggtgaacga tagcgtcttg tatggttttt aatacatcat cccgaaaacc
     1681 ttctttgaaa cgtgaaggat aagttacgat atgcagagcc tcgcgtaggt tatcaagata
     1741 gtttttgcga tcctgaggta agacggcctg gattttatgc aatgcggatg tgatatgggt
     1801 ttgaagcgaa gcatcagtca aagaacgtgt gagttctgaa cccactaaaa gcgcggcggc
     1861 ctcttcgtga gtaaacataa cgggaggaag gtggtaaccc tcaaccaaac tatagccttc
     1921 gccggcttcg gctgcgatag ggacaccggc ttcgttgagc gctttcatat cacgatacac
     1981 cgtgcgtagc gtaatatcaa aatgttcggc aatatctttt gcgcgaataa ttttacggct
     2041 gtgcagtaaa agcactatgg ctataagtcg gtcggtacga ttcatgtttt ttcaatttaa
     2101 agttgcaatc taatgcgttc gtacattaga ttcaatcatc cattcttata gaattaacca
     2161 atcattagga gagcaccatg aaaggcatga agcttagtca tctgcttttg tgtatttttc
     2221 tgatcggaga aggtttgata agtattcttg gattgaaatt tgacggtatt cattttgtca
     2281 tgggaggcat agctttagct gccggtgtgt taccgttttt ggataagtga aaatttaaat
     2341 aaaaaataaa atggtaaaag cgtcccaccg aaaggcagga cgcttttttt gtcgtatcac
     2401 gcgataaccg gaaatttcac aagcttataa gataaagcgt caggatgatt attttcaatg
     2461 ttatgtatcc tatgatcaaa gggggataca actctaattt ttgaacggaa ttattcatga
     2521 cgtgtctccg ttgaattata cttttgatct tacgtacatg atatagttta gaatgcaaaa
     2581 ccgaatttta gaatttcaaa gtcaacgatg attttcccgt catcaaggac attatcttga
     2641 atggatttgt cggtgtattt gaaataacga ccgacgctga gcgatgtacc ccagaagaaa
     2701 ccgcttttcg taaagtagcg ataaccgacg ccacataaaa cgccaagttt attttcagtg
     2761 actttatttg tagatatttc ttcgtcccaa ttccattcgc tcccctcata acccttgagg
     2821 cttgtgaaac gcaagccacc actaaaataa agaccgcctt ggcgatcacc gaggaaacga
     2881 cgatattgtg catctaaatt tataagtcgt gtcgagccgt ggcgtttgtc gccttgttcg
     2941 taaagaaatg ggaatgcgat ttctgcgcga cgatctatgg caaacatgga ataggttcca
     3001 ctgattgaaa ggcgatcgtc ggacattact agaaggcgag ccgggtttat ttcaaaccca
     3061 gcgccacgtg tggctaaagg atcttctttt tttacctcac tgtaaatcat tttttgatag
     3121 ctcatgagtg tatcaatctt actttcaata cgagcttgat tgtcctgagc actaagtgat
     3181 gagatattac ctagtttgag aattagcgca acggttaata gtatcttttt catttttccc
     3241 tccaagtttg gttttaaagt atgaatttga tttagtttca tattcgcatg aatttgagca
     3301 aatgcaatgc caacgtgtaa gttgtgcatt tataaggtgt ttttttagtt tatgagtgtg
     3361 aaaaaataca acgattgcaa gttttttatt gcaacaagat cataaagctc taacaaaaca
     3421 ctaataatcg cataataaaa cacagttata tttggaatag aaggaggtga taatatgaat
     3481 acgataccat tatatgtacg ctataccact cgcctgatag ttttaggcct agtagcggta
     3541 ttagtgacga tggttgccgt catagttcgg cggtaatcat cggttgagtt gagtttatgt
     3601 ttttctcttg tcaatcttcc atcggatatt ttcaatatat aattcattcc tcatttaaat
     3661 ttcagtttgc ccttagtgaa gttttttttc atattgggct ccatggaata tgaaaataaa
     3721 ctagatatat cgtttgaaac aaaaccgcag ttacccggta taggtacttc ttttttaatt
     3781 ctaattttgg gcatactgac gggattcgta tttatgggga ttttcgtcgt tataggtaaa
     3841 tttatcgtcc ctgcagaatc ggttttcgtt ttaaataaaa ttggattagt tcttggcacg
     3901 atgttatttg ttctgccggc attgatttat gcacgtatcc ataagctaga cgtcgtttta
     3961 ctatttcgca tgcggagagt aaatctatcg gttatgatta tttccgtcgt aactgcattg
     4021 ggacttgtcg tggttacgga tactttggat cgctggatgg ctccggcgat caatgcctgg
     4081 ctcgattcaa caataggcgt gctcagcccg gagctgatgt cagatcgtat tttggagcaa
     4141 ttggccgaag aaatgaaaat tcattcattt acggaagggt ttcttttgat tttaggcgca
     4201 gtaatcgctg cgggaatctg tgaagaaata ctattacgtg gacttttgca gcgatctctt
     4261 gaagcgcatt atcctgtatt gatatcggta gccatttcca gtcttgtatt cgcgttgatt
     4321 cattttaatc cctggggcgg catacagatt tttataattg cgatcgtgct tggcgccgta
     4381 gcctggcgaa cacaaagtat attaccgacg atcgtttttc atagtgtcaa taattttacc
     4441 gttctcattt ttaataatct tgagccgtca gccttattat ggtatggcga tgaacatcat
     4501 gtatttccat gggttagtat gggcggcttc atctgtacgt tattgggatt gtggcggatc
     4561 tggatattaa catccatcga aaaagaggaa acaaagtgaa ccgacgcgta actgtagcta
     4621 tcaccggggc aagtggtgtt atttatgccg ttcgtctttt gcgatatttt ctaacgcatg
     4681 cttacgaagt agattttatc atatcggatg ccggtaaaat taatttcaaa gttgagatgg
     4741 attgggattt agataaaaaa gggattgtcg aatttttaaa aaacaaattt ggccatgaca
     4801 tagacaaggg caaactgcgc gaatggccgg tacaggcgat agccgcgccg atggcgagcg
     4861 gttcaatgcg acgtctgggt atggtcgtag tcccttgctc catgaaaacg ctgtcgggca
     4921 ttgcgcatgg cgcatctact aatcttatcg aacgcgctgc cgatgtggcg ctcaaagaaa
     4981 attttccgtt gatcatcgtg ccgcgtgaaa cgccgatgaa tagtatccaa atagaaaaca
     5041 tgttgacgtt gtccaaagcc ggggcgcgga tcgtgccggc catgcccgcc ttttatcaaa
     5101 aaccgcaatc aatagacgat attgccgatt ttatagtagc tcgaattttg catatgctcg
     5161 gcattgacga tcatgggctt tttcaaccat ggggcggtta accgcatgca acgaaaacgc
     5221 agagaagacg atgatatgcc ggagtcatct gatgatcacg ccaaggaaat ccgaattcca
     5281 gtcaagccgg gtcacgagcc caagaaatcg gccctgaata aaaccaacca ctcttccgca
     5341 tggatattgg ctctgaagtt gttttctgcg gtcgtgtttt tgcttttgac ggtagtgatc
     5401 ttttttgctt attattttcg cgacgatctg ccttctatcg aacaactcga agtgtacgaa
     5461 cccaaacttt ccacaaaagt atattcagcg gacgggaaag tcatcaagga gttttttcgg
     5521 gaacgtcgaa cacgcattcc gattcaggaa atccccaaat ccatcaccga agctttgctc
     5581 gcgacggaag atcatcgctt ttacgatcat tggggtatgg atattattcg tttcgtcaag
     5641 tcggcgatgg tcaatacgtt gacgctgagt tccagtgaag gatttagttc actcacgcaa
     5701 caactcgcgc gaaatctcta tctgagcaaa gaaaaaacgc tctcccggaa attcaaagaa
     5761 gtattgaccg ccatacagat cgaaaaaaca tattccaaaa acgagattat ggaaatgtac
     5821 ctcacgcaca tgtatttcgg tcatgatgcg tacgggatac atgctgccgc caagattttt
     5881 tttcacaaag atgtacggga actgtcttta gcggaatctg cgtacctggt cggccatttg
     5941 caggcccctt cattttatat gaataatatc caagcggcca atactcgcaa aaacacagtg
     6001 ctttatcgta tgagggaggt tggctatatc ggtgagactg aattttcggc ggcgcgtcag
     6061 gaaaagattc agacctttcc tcgccgtgac gaggacgtga tcggatacgc tccgtatttt
     6121 gcagaaaatg ttcgtcgtga attagagtat ttgtccgaca gtttgcattt taatgtatac
     6181 gaagacggac tcaatgtttt tacgacattg gatacgcgcg cgcaggccgc ggctgaaaaa
     6241 gcatatgagg aaaatgtaaa agaacggttt ggtcctttgg ataaatttgg cgcggaatat
     6301 cttgccgcca atgcgcaaca gtatttacct aaatatatgc gtcgcgaagg tgtttcgccc
     6361 gagcgcatcg aacgactgct cgccagtccc cgagcggtgg attctctcag tcgcttctac
     6421 ggcaccgtgc agtccggttt tgtagccgtc gatccttcca atgggcatgt gttggctttg
     6481 atcggcgggc gtgattttaa aaaatataaa ctcaatcacg taacacaaat tgcgcgtcaa
     6541 cccggctcta cgttcaagcc ttttttgtac acggcagcca tagataacgg gtattcgcca
     6601 aactttaaat tgctcaatca agacgtggtg atcattacgg aagacggtaa acgatggaca
     6661 cctcaaaact atgacggagg ccggggtggc ccgaccacgc tgagggatgc tttagcaact
     6721 tcactcaatc ttgtcgccat acggcttatg caagaggttg ttccgccggc cacggtgatc
     6781 acgtatgcac aaaaaatggg tattcagacg acgctgcaac ctgtcgatgc actagccctc
     6841 ggcgtttttg atgtggtacc gatgcagatc gtatctgcgt acggtgtttt tgccaacaaa
     6901 ggtatccgtg ttgatccggt ttttattaca cgcattacgg acaaagacgg taatcttttg
     6961 tatcagcata ttcccaagta taaagaagtt ttgagtgcgg agacggccta catcatggcg
     7021 gaaatgatga agtccagtat tgatagtgaa cgcgggtcgg gacgattagc acggcgattc
     7081 ggatttaagc gtccggcagg agtaaagacc ggtacaacgc aaaaatggtc cgacggatgg
     7141 tacatcggct atactccgca aattgtcgcc ggtgtttggg tgggctatga tacgtatgaa
     7201 ctcaatctca gtgcgcgtaa tcccggcgaa gtgatagccg cgccgatgtg gggaagattt
     7261 atgttggatg cgcacgaagc catggggtta ccggaagcgg atttcgaaat gccgccaggg
     7321 gtcgttcgtt tggaaatatg cgaggattcc ggtcaacttg cgatcactgc gtgtccaaaa
     7381 aaatacaaag aagtttttaa tgccaagttt caacctacag atccctgtgc ggtacacaaa
     7441 acaacagtag gcaaaaagaa aaaagggcgg gggtattaag accaatgccg gttaacgaag
     7501 ttcaactcaa cgcctatgcc aaaataaatc tcggcttacg tattcatgaa aaacgaggcg
     7561 atggttatca caatatagaa accgttttta aaatcataag ccttcacgac cgcgtcgcga
     7621 ttcggttgaa tgatagaggt ggcgcacgtc tgacatgcgg gctcgccggt gtgcctacgg
     7681 atgaatccaa tatcatgatg aaagcagtac gtagcctgga aaaaaaatcc ggtcggccaa
     7741 ttcataccga ta
//
LOCUS       DAOQNQ010000145         7749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3531_length_7749_cov_5.132181, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000145 DAOQNQ010000000
VERSION     DAOQNQ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3531_length_7749_cov_5.132181"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="PK754_09690"
     CDS             <1..932
                     /locus_tag="PK754_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNB09819.1"
                     /translation="GNPFFNDDFFSQFFPKQKYYDKVKGLGSGFIISSDGYIVTNHHV
                     INGAVEIVVTLTDGTKYPAKIVGGDKTTDIALLKIEPERKLKYLEFGDSEKLLIGEWV
                     IAMGNPFGLFDINSQPTVTVGVISATNRDFGLVDNDHVYQNMIQTDAAINGGNSGGPL
                     LNSNGEVIGMNTFIYSGSSTSKTSIGIGFAIPIHRIDKIISELKSNGKVDRYFTMGFR
                     YQQVDQMIAKYLGLKELRGIVVSEIIKNGPAENAGLLVGDLIVKINDKPINSEYDFRK
                     ITLGDDLRAGDVVDVEVLREKKRLTFQLKLGKV"
     gene            944..1834
                     /locus_tag="PK754_09695"
     CDS             944..1834
                     /locus_tag="PK754_09695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNB09820.1"
                     /translation="MKIGFIVNPRAGTHHNAYEEVSALAGKLVALGHTITLHKTQGPG
                     DATVMARNLVQQGYDVIASAGGDGTANETAQGVMDSDSAFTIIPYGSGNGLARGLAIP
                     LKPSAAVQGIPNGKVRRIDVGQLIDGSQVRLFFGFAGIGYDAYIGRLFNQRKGRRGLL
                     PYVYLSLTAYNGYAPVALTLKADDVLYSGKPFILAIANTDEYGNGARIAPNAVPDDGV
                     FDISMIQSMSFVKGLLNGWRLFNGTIDKVKDVVSLRVQEAEVIPSGKIYYHMDGEARE
                     TNNTLHFKILPGRLKVLVPN"
     gene            1899..2603
                     /locus_tag="PK754_09700"
     CDS             1899..2603
                     /locus_tag="PK754_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNB09821.1"
                     /translation="MNDNKSVTRKLETLKIVPIFSGLGIKDLEKIANVAFQRSYTKEQ
                     VVLFEDEGGQTLFIVMTGQVKISRISEDGREVILAVMAEGDFFGELSLLDGQSRSANV
                     VCTKDAEMLLINRDDFLNLLSEFPQIAIQLLRELASRMRKSDSQIKNLSLKNSTGKVA
                     GTIARLAEDIGIPNNGSTIEIPNLPTQQDLANMSGTSRETISRVLQRFINDGYVSKDG
                     NKLVIRDFEKFKKDLV"
     gene            2704..4818
                     /locus_tag="PK754_09705"
     CDS             2704..4818
                     /locus_tag="PK754_09705"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB09822.1"
                     /translation="MKKKATIVICDPHPDSASALTEILVSNDYALVHAHDGHDVLDKV
                     KSLDYDLALIATDTYAMDGFQVCRLLKESERTRHIPVMLASGMSDKVTRNRAIQAGAD
                     EFITKPYNRVELLSRVVNLLRIKHLHDRLENLISEKETEKRKLEERTRELQLLSDIAR
                     IVISLKEQKIVLAEIIDKISAAFGVEVALLITREGSDWAIHTLSSHISEKHIGTLLPH
                     GGSIFEYIERYEKPMIVNDAVRDERIPAAIYALTPNPLRAAICTPIYIRGQLMGVLMI
                     INKKTQMGFEHGDLSLLMTVSGQIALAMENMQLFNKLSDFNRNLQDQIRQATHALVDL
                     KNFNESILHNVSAGIITADFSGRILFVNRAAKSILGFSDNELLDHKMETIFGREEASR
                     MLHPTVENESAPGNAEVQVRQKDGTRLFLGFTTTMRYDVDHNMVGYIISFKDISLVKS
                     MHETIMRMDRLAASGALTSAIAHEIRNPLAGIKTIAQALQKELPPDDPRHEYVYRIIK
                     QINRLNDLLKAFFTYAKPVRPEKKACALPELIQEIKALLKARAETDHIEIHEAYDEKL
                     APIEVDENQFSQVMLNLMMNAMDAIGQGGRIAIRAQSVQRTPLNNYDLPITMAEIRVS
                     DTGKGIPQEQVRSIFDPFFTTKPGGIGLGLSIVYRIVQEHGGEIKVESAPGKGTTFIM
                     HIPCAEKSKSILENRHTLTTTI"
     gene            4832..5923
                     /locus_tag="PK754_09710"
     CDS             4832..5923
                     /locus_tag="PK754_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB09823.1"
                     /translation="MQKKVLIVDDEEDLTWSISKNLSKDKDSFDLICVNNAQEALNVL
                     SQLPVDLVVSDIKMPGMSGLELLVKIKETYSTTKVIIMTAFGSAEVQREATDRGSLYY
                     IEKPFEIEELRTLIHRALTEKKGFDGKVSDFQLSDLIQMNILGRMKAALVVNKDEEKG
                     TLYFSDGNIVHAECGSIIGEEAFYKILAWENGRFEFRKGEDPEKETIDKGWQSLLLEG
                     MRRKDEYSPETKTQIKEESRQEMLEKIKTSIAEFIKIKGVDLVAVIDDAGFSRASLFN
                     EKKEERMDITLLSTIIPPGLEYLLKVDTELKGEGLRFITLDFNKKTIFMAPVPGRKEW
                     LFVLCEPEVNLGGLRMAFKKQAPLLSELL"
     gene            5930..6781
                     /locus_tag="PK754_09715"
     CDS             5930..6781
                     /locus_tag="PK754_09715"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNB09824.1"
                     /translation="MIEKTSELWADLHMHSYYSDGILSPEAIVKYAAKIGLSCIAITD
                     HDTVKAVDEATEAGNKHNVEVLTGIELSTTWDGRDIHVLGYCFDHTDAALDVQLQLYR
                     DERRHRAIRIIDRLQKQGMEITIEEVEKKAGRGVIGRPHIADVMLSKKMAYNINDVFA
                     RFLGPDGTAYEDKYKISPRFAVELIEKAGGVAVLAHPTLNMKETHVHDMIKAGIHGIE
                     CIHPKHTADSTAYYRRLTVQYGLEESGGSDCHGRGQVPLIGQYRVPYNVVQRLKDRAQ
                     ALRKVNA"
     gene            6778..>7749
                     /locus_tag="PK754_09720"
     CDS             6778..>7749
                     /locus_tag="PK754_09720"
                     /inference="COORDINATES: protein motif:HMM:TIGR04552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04552 family protein"
                     /protein_id="HNB09825.1"
                     /translation="MIDPKKKAPLYVPPVKNIFSPQEILYLSDMRLSDLEAIRLVLNG
                     DSVIDWFRLDFHTPDQVQLFLQVNGYDLTNVQDRKRIEKIHREAVEYLDSKYPHRIPK
                     EIRPESATIEQLLYLASQSARKNKHQFYACMVLKIMHTINHLDARELFHRCQMRASDL
                     YFAVERKVEHAVTTMKNNEFHIVDFYGSHKDKMSMVTKLMAKKENHAAAIYDRIRFRI
                     ITQTLEDILPVLYFLGRTICPFNYVIPGSSHNNLIAFAELVEMFPNLRSYAKFLRRKD
                     THAFDPAEVSKENKFSSTSYRSINIVMDIPIRVDDFLYTPDFSEFGRI"
BASE COUNT         2222 a         1648 c         1859 g         2020 t
ORIGIN      
        1 acgggaatcc tttttttaat gacgattttt tcagtcaatt ttttccgaag caaaaatatt
       61 acgataaagt caaaggcctt gggtcgggct tcatcatctc atcggacggt tatattgtca
      121 ccaatcacca tgtgatcaat ggcgctgtag agatcgtcgt tacattgacc gacggtacta
      181 aatatcccgc taaaatagtc ggcggtgata aaactacgga catcgcgtta ctcaagatcg
      241 aaccggagcg caaactcaaa tatctcgagt ttggcgactc ggaaaaactg cttatcggcg
      301 aatgggttat tgccatgggc aatccgtttg gtttgtttga catcaatagc caacctacgg
      361 taacggtcgg ggtgatcagc gcgaccaatc gtgatttcgg gctcgtagat aacgatcacg
      421 tctaccaaaa catgatacaa accgacgccg cgattaacgg cggtaatagc ggtggacctt
      481 tattaaacag caatggcgaa gtgatcggga tgaatacttt catttacagc ggaagtagca
      541 catcaaaaac gagtatcggt atcgggtttg caataccgat tcatcgcatt gataaaatca
      601 tatccgaact aaaatccaac ggtaaagtgg atcgttattt tacgatgggt tttcgctatc
      661 agcaggtgga tcaaatgatc gccaaatacc ttggtctaaa agaattacgg ggtatcgtcg
      721 tttccgaaat tatcaaaaat ggcccggctg aaaatgccgg attgctggtc ggcgatctaa
      781 tcgtaaaaat caatgataag ccgatcaatt cagaatacga ttttcgaaaa atcacgttag
      841 gcgacgactt gcgtgccggc gatgtcgttg atgtcgaggt gttacgcgaa aagaagcgcc
      901 ttacgtttca gttaaaattg ggaaaggtgt aatcgaataa tagatgaaaa taggttttat
      961 cgtcaatccc cgcgcgggaa cgcatcataa tgcatacgaa gaggttagcg ctttggccgg
     1021 aaaactggtt gctctgggcc atacgataac attacacaaa acccaaggcc cgggcgatgc
     1081 gacggtaatg gcgcgaaatt tagtgcaaca aggatatgat gttattgcat cggcaggcgg
     1141 agacggtacg gccaatgaaa cggcgcaggg cgttatggat tccgattcgg cttttacgat
     1201 tatcccttac ggttcaggca acggtttggc acgcggattg gcgataccac ttaagccatc
     1261 cgcggcagta caaggtattc cgaacggaaa agttcgccgc attgatgtcg ggcaactgat
     1321 tgatggctca caggtgcgat tatttttcgg atttgccggt atcggttacg atgcctacat
     1381 cgggcggctt tttaatcaac gcaaagggcg ccgcgggctt ctgccgtatg tatatttatc
     1441 gcttacggcg tacaatggtt atgcgccggt ggcgcttaca ttgaaagcgg atgatgtgct
     1501 gtactccggc aaacccttta tcttagcgat agctaatacg gatgaatacg gtaatggtgc
     1561 gcgtatcgca cctaatgccg taccggatga tggtgtcttt gatatttcca tgatacagtc
     1621 catgtcattt gttaaaggtc ttttaaacgg atggcgttta ttcaacggta cgattgataa
     1681 agtcaaagat gtggtcagct tgcgtgtgca ggaagccgag gtgataccgt ccggtaaaat
     1741 atattatcat atggatggcg aagcgcgcga aacaaataac accttacatt ttaaaattct
     1801 gcccgggcgc ctcaaagtgc ttgtacctaa ttagttcttg attgcagcat gcgaatatcg
     1861 tactttgtgc cgttccaaaa tcccttcaaa tagtacctat gaacgacaac aaatccgtaa
     1921 cgcggaaact tgagacgcta aaaatagtac ctattttttc cggccttggt attaaagacc
     1981 tggagaaaat agcgaacgta gcttttcaac gcagttacac aaaagagcag gttgtgcttt
     2041 tcgaagatga gggcggccag acgcttttta ttgtgatgac cgggcaagtc aaaatttcgc
     2101 gcatcagcga agatggccgc gaagtaatat tggctgttat ggcggagggg gatttttttg
     2161 gcgaattgtc gttgctagat ggccagtcgc gttcggctaa tgtggtttgc actaaagatg
     2221 cagagatgct tcttattaac cgggatgatt ttctcaactt acttagtgaa tttccgcaaa
     2281 ttgctatcca acttctgcgt gaattggcaa gtcgtatgcg gaaatccgat tctcagatta
     2341 aaaatctatc gcttaaaaat tcgaccggca aagtagccgg cacaattgca cgtttagccg
     2401 aagatattgg tatccccaac aacggcagta ccatcgaaat tcccaattta ccgacgcagc
     2461 aggacttggc caatatgtca ggaacaagtc gcgagacgat ctcccgtgta ctacagcgtt
     2521 ttatcaacga tggttatgta tctaaagatg gtaataaact tgtgattaga gattttgaaa
     2581 aatttaaaaa agatttggtt taagaataaa tatggaatgc agcccaaagc cagccctacg
     2641 gttggctttt gtttttttgg cttttgaaaa ggcgcttccg tatattgccc gcgcataaat
     2701 catatgaaga aaaaagccac tatcgtaatt tgtgacccgc atccggactc cgcgtccgca
     2761 ttgacggaaa ttctcgtctc caacgattat gctttggttc atgcacatga tgggcacgat
     2821 gtgctcgata aggtaaaaag tctcgattat gatctggcgc tgattgctac cgatacgtat
     2881 gcgatggacg gttttcaggt gtgccgtttg cttaaagaga gcgaacgaac gcggcatatt
     2941 cccgtgatgc tggcttccgg tatgtctgat aaagttacgc gtaatcgtgc gatacaagcc
     3001 ggtgcggacg aatttatcac gaaaccgtac aatcgtgtcg aactgttgtc gcgtgtcgtc
     3061 aatctcttgc gtatcaaaca tctgcacgat cgtttggaaa atttgatatc cgaaaaagaa
     3121 accgaaaaac gtaaattaga agaacgcacc cgcgagctgc aacttctatc ggacatagcg
     3181 cggattgtta tttcgctcaa agaacagaaa atcgtattag cggaaattat tgataaaata
     3241 tcggccgcgt ttggcgtcga agttgctctg ctcataacgc gggaaggatc ggactgggcg
     3301 attcatacat tgtcatctca tatttccgaa aaacacatcg ggacgttatt accgcatggc
     3361 ggttcgattt ttgaatatat tgagcgttat gaaaaaccga tgatcgtcaa tgatgctgtt
     3421 cgcgatgaaa gaatacccgc ggcgatttat gcgctgacgc ccaatccgtt gcgtgcagcg
     3481 atatgtacac ccatatacat acgcggtcag ctcatggggg tactgatgat catcaataaa
     3541 aaaacacaga tgggatttga acacggcgat ctctcattgc tcatgacggt atcgggtcaa
     3601 atcgctttag cgatggagaa tatgcagctt tttaataagt tgtcggattt caatcgtaac
     3661 ttgcaggatc aaattcgaca ggcgacgcat gcgttggtgg atttaaaaaa ttttaatgag
     3721 agtattttgc acaatgtcag cgccggtatc atcacggctg atttttccgg gcgcatttta
     3781 tttgtcaatc gtgccgccaa atccatttta ggattttccg acaacgaatt gcttgatcac
     3841 aaaatggaaa caatttttgg tcgcgaagag gcctcacgca tgcttcatcc gacggtagaa
     3901 aacgaatccg cgccgggcaa tgcggaggtg caggttcgac aaaaagacgg cacacggctt
     3961 tttttaggtt ttacgaccac tatgcgttac gacgttgacc acaacatggt gggttatatt
     4021 atcagtttca aagatatttc tttggtcaaa tccatgcatg agacgatcat gcgaatggat
     4081 cgtctcgccg catcgggcgc attgaccagc gctattgcac atgaaattcg caacccgttg
     4141 gcgggcatta aaaccatcgc acaggccctt caaaaagaat tgccgcccga tgatccgcgc
     4201 catgaatatg tttatcgcat tatcaaacaa atcaaccggc tcaacgattt gcttaaagct
     4261 tttttcacct atgccaaacc cgtgcgcccg gagaaaaaag cctgtgcgct gccggaactg
     4321 atacaagaaa tcaaggccct tttgaaagcg cgtgccgaga cggatcacat cgaaatacac
     4381 gaagcgtatg acgaaaaact cgcgcccatc gaggtggatg aaaatcaatt ttcgcaagtc
     4441 atgcttaatc ttatgatgaa cgctatggat gcgatcggtc aaggagggcg catcgcgatc
     4501 cgtgctcaat ccgttcagcg tacaccgctg aataactatg atttaccgat aaccatggcg
     4561 gaaatacgcg tcagcgatac ggggaaaggt attcctcaag agcaagttcg ttccattttt
     4621 gatccttttt ttacgactaa acccggtggt atcgggttag gtctttcgat cgtgtatcgt
     4681 atcgtgcagg agcacggagg tgaaatcaaa gtggaaagtg caccgggtaa aggtacaaca
     4741 ttcatcatgc acattccttg tgcagaaaaa tcaaaatcta tattggaaaa ccggcataca
     4801 ttgacaacaa ccatctaacc gttaaatcca tatgcaaaaa aaagtactca tagttgacga
     4861 cgaagaagac cttacgtgga gcatttccaa aaatctgtct aaagacaagg atagttttga
     4921 cctgatctgt gtcaataacg ctcaagaggc gcttaatgtt ctttcgcaat tacctgtgga
     4981 tctggtagta agcgacatca aaatgccggg tatgtccggg ttggaactgc ttgtaaaaat
     5041 caaagaaacg tattcgacaa ccaaggttat cattatgaca gcctttggtt cggccgaagt
     5101 acaacgtgaa gctacggatc gcggttcgtt gtattacatc gaaaaaccgt ttgaaatcga
     5161 agaactgcgt acgctcatac accgtgcact gacggagaaa aaaggatttg acggtaaagt
     5221 ttccgacttt cagttgtccg atcttatcca aatgaatata cttgggcgta tgaaagcggc
     5281 cttagtggta aacaaagatg aagagaaggg aacattatat tttagcgacg gaaatatcgt
     5341 ccatgccgaa tgcggttcga ttatcggaga agaagcgttt tataaaatat tggcatggga
     5401 gaatgggcgt tttgaatttc gtaaaggtga agatccggaa aaagagacta tagataaagg
     5461 gtggcaaagt cttttacttg aaggtatgcg ccgtaaagac gaatattcgc ccgagacgaa
     5521 aacgcagatc aaagaagaat cgcgtcagga gatgctcgaa aaaataaaga catctatagc
     5581 ggagtttatc aaaatcaaag gcgtggatct cgtagcagtg attgatgatg ccggattttc
     5641 gagagcatcg ctttttaatg aaaaaaaaga agagcgtatg gatatcaccc ttctgagtac
     5701 gatcataccg cccggcttgg aatatcttct gaaagtggat acggaactga aaggcgaagg
     5761 acttcgtttt atcactcttg atttcaataa aaaaaccatt ttcatggcgc ccgtgcccgg
     5821 tcgcaaagaa tggttatttg tcttatgcga accggaagtc aatctcggcg gacttcggat
     5881 ggcttttaaa aaacaagcgc cgctattatc ggaattactc taaggattta tgatcgaaaa
     5941 aacgtccgaa ctttgggccg atctgcatat gcattcctat tattcggatg gcatattatc
     6001 gccggaagcc atcgtaaagt acgccgctaa aatcggcctg agctgtatag cgatcaccga
     6061 tcatgacacg gtaaaggccg tggatgaagc gacagaagcg gggaataaac ataatgtgga
     6121 agtcctgacc ggcatagaac tcagtacgac ttgggatgga cgcgatattc acgttttggg
     6181 atattgtttt gatcacaccg atgcggcatt ggatgtacaa ctgcagcttt accgtgatga
     6241 gcggcgccac cgggccatac gtattattga ccgtcttcaa aaacaaggca tggagattac
     6301 gatcgaagag gtcgaaaaga aagccggtcg cggagtgatc ggcagaccgc atattgccga
     6361 tgtgatgcta tccaaaaaaa tggcgtacaa tatcaacgat gtttttgcgc ggtttctcgg
     6421 cccggacggt acggcctatg aggataaata taaaatttca ccccgttttg ccgtcgagct
     6481 catcgaaaag gccgggggtg tagccgtttt ggcgcatcct acgcttaata tgaaagaaac
     6541 gcatgtccat gatatgatca aggccggtat ccacggaatc gagtgcattc atcccaaaca
     6601 tacggccgat tcgaccgcat attaccggcg ccttaccgta caatacgggc tcgaagaaag
     6661 cggcggttcc gattgccacg ggcgagggca agtgccgctc ataggtcagt accgtgtccc
     6721 gtataacgtc gttcagcgac tcaaggatcg cgcacaagct ttgcgcaaag taaacgcatg
     6781 attgatccca agaaaaaagc gccattgtac gttccgccgg tgaaaaacat tttctctccg
     6841 caggagatac tctatctcag cgatatgcgc ttgagcgatc tcgaagccat tcggttggtg
     6901 ctcaacggag attccgtgat tgattggttt cgcctggatt ttcatacacc ggatcaggtt
     6961 cagctttttt tacaggtcaa cgggtacgac cttaccaatg tgcaagaccg caaacgtatc
     7021 gaaaaaattc atcgcgaagc ggtcgaatat ctcgacagta aatacccgca tcgcataccc
     7081 aaagagattc gaccggaatc ggccaccatc gaacaacttt tgtatctcgc ttcacagtcc
     7141 gcacgaaaaa ataaacatca attttatgcg tgtatggtcc tcaagatcat gcatacgatc
     7201 aaccatctgg atgcgcgcga actttttcac cggtgccaga tgcgggcttc ggatctttat
     7261 tttgcggtcg aacgaaaagt ggaacatgcc gtaacgacga tgaaaaataa cgaatttcat
     7321 atcgttgatt tttacggaag ccataaagac aagatgagta tggtaaccaa actgatggcg
     7381 aagaaagaga atcatgccgc ggctatttat gatcgcattc gttttcgcat tatcacacaa
     7441 acgctcgaag acattttgcc agttttgtat tttttagggc gaacgatttg tccgttcaat
     7501 tacgttattc ccggcagttc gcataataat ctgattgctt ttgcggagct tgttgaaatg
     7561 tttcctaatc tgcgatcgta tgcaaaattt cttcgtcgaa aagatacgca cgcatttgat
     7621 cctgccgaag tatcaaaaga aaataaattt tcgagtacca gttatcgttc gatcaatatt
     7681 gttatggata taccgatacg tgtggatgac tttttgtaca caccggattt ttccgaattt
     7741 gggcgtatt
//
LOCUS       DAOQNQ010000146         7694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3563_length_7694_cov_6.925776, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000146 DAOQNQ010000000
VERSION     DAOQNQ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7694
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3563_length_7694_cov_6.925776"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PK754_09725"
     CDS             <1..436
                     /locus_tag="PK754_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09826.1"
                     /translation="CFALSDSDFVVVGFDGTIRSSTNAGRNWVSETSGTTQHLRGISK
                     TQNGDMIVVGYSGVLLRKTSGSSEWASSTSHTTNNLFGVDCATTSGSVFVSGTSVMLE
                     SSDNGNVWHTNDVPTSVDLYDIYTANDGGGIAVGQNGTILIK"
     gene            complement(493..1110)
                     /locus_tag="PK754_09730"
     CDS             complement(493..1110)
                     /locus_tag="PK754_09730"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNB09827.1"
                     /translation="MRKMIFSLLMMGVIATTQNINAQEARPLPDLGNGEEVDIFFFED
                     GEGFMADAPMMLDMPMMNVIMEDETGLEAEFPPMGMGDGAAEKIDLSKDQKDKIRKIR
                     GDVKKQNITLRGNLELKQIELHELLDAENPSKEQIAAKVKEIESLKTQMKMNHINARL
                     DCRNILTKEQREKMDQMRSERRDRWHEKRGHKAMKFKRFFHGGME"
     gene            1257..1988
                     /locus_tag="PK754_09735"
     CDS             1257..1988
                     /locus_tag="PK754_09735"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB09828.1"
                     /translation="MNSANQNKLCLVTGALGHLGQALSECLLEEGYHVVGIGSGNTLN
                     ENFKKSEFLKYVSGKKTWTFYSVNLTSEEEVTQLFQSVSQNNNSLLALVNLVGGIYPW
                     SMLHEVEAKTWDAMIELNLKTTFLCSREAIKVMIPQRCGKIINIGALAGIRGNTMAGP
                     YGAAKAALINMTETMAEENKRNNIQVNAVVPGIIDTPTNRQSMPDADFSTWVKPQSIA
                     EVISFLVSEKSNALTGSVLRVTGKL"
     gene            1995..2645
                     /locus_tag="PK754_09740"
     CDS             1995..2645
                     /locus_tag="PK754_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNB09829.1"
                     /translation="MKSITDQPEYSFGEELAHAITHGIGAILSIAGLTLLVAFSALRG
                     DAWHVVSSAIFGTTLVLLYSASTLYHSFPQPKVKRIFRIMDHSAIYLLIAGTYTPFLL
                     VSLRGAWGWTLFGVVWSAAIAGVWFKVFHTGRFPKLSTTLYIVMGWVALVAVKPMMEN
                     IPLGGLILILAGGLTYTVGVVFYVMHRMIYHHAVWHLFVLGGSILHFFAVLLYVIP"
     gene            2679..5555
                     /locus_tag="PK754_09745"
     CDS             2679..5555
                     /locus_tag="PK754_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09830.1"
                     /translation="MKTRYLICHLVAFFLSSHLSAQISPLGTEQNPPGIAWKEIETKH
                     FRVVFPEEITQDAQRVATTLEYLYEPVSHSLKKKPHRITVILHNQNIISNGFVTLAPR
                     RSEWYNTPPQTAFTGTNDWYNLLSVHEFRHVVQFDKMNRGLIALMYGLFGETGTLAAS
                     FLSVPSWYWEGDATGTETALTQSGRGRLPSFNMPMRTMLLRGPQQNYYQMLLGSYKNY
                     FGGAYEMGYYMTSYVKRQHGSDMWSDILRRNSNFPFFYTFSWSMKAKTGNGAPNTFEN
                     MSQELKDLWSTQELSYKSDLTTIEVRYDNGFMNPFPNNPKTLTDGSVVFFDYNLGEAP
                     GIKIKTTDGAISHITRSTWSGNFSASQNKVIWVDPDWDLRWGLKNGSRVVVWDATTRN
                     TQTLLTNTRLTYVAMSADGQKFAAVEHSRSNKSSIILFDTQTGNIIKKLSNPNNDVII
                     NPSWSSDNKHIVYVRQTLHDGKGVTLIDIENDATTDVLPAQHTMNYTNPVIWNDWILL
                     NAHFDGVDNIFAFHKYERTWKQITHSSLGAFNPFVSAGNRLVFDEYTVDGYIANSLLL
                     DTNRWTNVDIQSRKNDGYYEPLLQQELGTIRFDSITPGTFAVKKYNSLSDLINIHSWS
                     PTLNPRQYGVSVFSQNIMSTTRLSASYNYDRNEKTSGGEVGASYGGFYPIADVTAKLG
                     SRASTYTVTTIKNGKKTEKLKWYGWNEKSLSAALRLPLNFSSQGWQRYFSASAGFSYT
                     KVSDQKISFAFDQNNGVLQSIFLSGFYYNATQPAAYVYPQWGQRIWITYSKAPFKDHY
                     NGSQFFAQGDLFFPGLLKRHSGWIQGQFERQKTGANYRYASWMIFPRGYSYIYHDAIK
                     KLGATYTVPLMYPDTDFLAMFYIKRIYGDVFGDYGVGQDYSGPNVKTKYRSAGAGGYA
                     NMHFFTLPVEFSVGYRYAYAFDKKNTENNKHAQPQIVIALTGQF"
     gene            5621..6088
                     /locus_tag="PK754_09750"
     CDS             5621..6088
                     /locus_tag="PK754_09750"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNB09831.1"
                     /translation="MSYIVWLHLILAVNSNTNDLVWHKFDQGLALAAKENKAILIDVY
                     TDWCGWCKKMDQDTYSDPRVVKYLRENFVLIRMNPETDGPVSYEGEQYPAAAFAQSMQ
                     VTGYPATAFFESNGKMITMVPGYIQSDDFLNILKYMAEKKYYTVSYEDFLKAK"
     gene            complement(6092..6646)
                     /locus_tag="PK754_09755"
     CDS             complement(6092..6646)
                     /locus_tag="PK754_09755"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNB09832.1"
                     /translation="MNLLITVSFCLMLSVLLYFITGRKLELKNRKIWLGSMIVVLLLA
                     TSIMFFAQKELDNYQAYRAWLPTPARVISAQVVGKRAFRPEIIYTYTVNGFSVTDTTD
                     MEVPGFGSKSNRFNVARNEVASVREDSTFVVYYNPANVVEHLSHIRPPHTIYIMYSTG
                     GCFFIAGTYIFLVGLFQTKKNKPD"
     gene            complement(6716..7498)
                     /locus_tag="PK754_09760"
     CDS             complement(6716..7498)
                     /locus_tag="PK754_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNB09833.1"
                     /translation="MSYKNILFEVKNKIAYLTINRPDKLNALNTETFMEIMDAMKKAN
                     EDASVSGVIVTGSGEKAFVAGADISELAVQTPVTAKEFALRSEVVLCYIERMHKPVIA
                     AINGFALGGGCEFAMACHMRIASENAKFGQPEVNLGLIAGNGGTQRLPRLVGKGRAIE
                     ILCTGDMISAEDAFRIGLANRIVKKEELIPTCEKILQTIASKAPVAVKLTLEAVQSGM
                     EMTLAEGLNFEANLFGLVFSSDDMKEGTKAFLEKRKANFQGK"
     gene            complement(7518..>7694)
                     /locus_tag="PK754_09765"
     CDS             complement(7518..>7694)
                     /locus_tag="PK754_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNB09834.1"
                     /translation="CKGYARVDFRMNQSGELFCLEINTLPGMTATSLVPKAAKAGGME
                     FEELVEKIIRLALP"
BASE COUNT         2152 a         1674 c         1551 g         2317 t
ORIGIN      
        1 atgttttgca ttaagtgatt ccgattttgt tgtagtcggc tttgatggaa caatacgcag
       61 cagtacgaat gccggtcgca actgggtaag cgaaacaagc ggaacaaccc aacatctgcg
      121 cggcattagc aaaacccaaa acggggacat gatagtcgtc ggatacagcg gtgtcctact
      181 tagaaaaact tccggttcat cggaatgggc atcctccaca agccatacga ccaataatct
      241 ttttggtgtg gattgtgcta caacttcagg gtcagttttt gtgtcaggca catcggtaat
      301 gttagaaagt tccgataacg gtaatgtatg gcataccaat gacgttccaa cctctgtcga
      361 tctatatgat atttataccg caaatgacgg gggaggtata gcagtgggtc aaaacggaac
      421 tattctaatc aaataaaaaa aagcgaaacc attttcacgg tttcgctata ccagaagaga
      481 gggcggttat ttttattcca taccgccatg gaaaaagcgt ttaaatttca ttgctttgtg
      541 cccacgcttt tcgtgccagc gatcgcgtct ttcggaacgc atttgatcca ttttttcacg
      601 ttgttctttg gtcaaaatat tgcggcaatc caaccgtgca ttgatatgat tcattttcat
      661 ttgcgttttt aagctctcga tttcttttac tttggctgca atctgttctt ttgaaggatt
      721 ttcagcatcc aaaagttcat gcaactcgat ttgcttcagc tcaagatttc cgcgcaacgt
      781 gatattttgt ttttttacat cgcctctgat ttttcggatt ttatcttttt gatctttgga
      841 taaatctatt ttttcggccg caccgtcacc catacccatc ggcgggaact ctgcttcaag
      901 acctgtctcg tcttccataa tgacattcat catgggcata tcaagcatca tcggtgcatc
      961 cgccataaaa ccttctccat cttcaaaaaa gaagatatca acttcttcgc cgttccctag
     1021 atccggcaat ggtcttgcct cttgtgcatt gatattttga gttgtagcta tcacccccat
     1081 cattagcaaa ctgaaaatca tctttctcat aaataaactc cttaattttg ttttaattac
     1141 atgtattgga cgaatgccgt taaaattggt ttaataccga actgtcattt aattgggatg
     1201 gtggttcgta aaattactta ttggatttgt cagttttaat attgagttta tctataatga
     1261 attctgctaa tcaaaataaa ttgtgtctgg ttacgggcgc attaggtcac ttagggcaag
     1321 cgctaagtga atgccttctt gaagaaggtt atcacgtagt aggcatcggc tcgggtaaca
     1381 cactaaatga aaattttaaa aaatccgaat ttttgaaata tgtatccgga aaaaaaactt
     1441 ggacatttta ctcagttaac ctgaccagcg aggaggaagt cactcaatta ttccaatctg
     1501 tctcccaaaa taacaattcg ttattggctt tagttaacct tgtcggcggt atttatccgt
     1561 ggtctatgtt gcatgaagtt gaagccaaaa catgggacgc aatgatcgaa cttaacctca
     1621 aaacgacttt tttatgttcg cgggaagcca tcaaagtaat gattcctcaa cgttgtggta
     1681 aaataattaa catcggcgcg ctcgccggta taaggggtaa tacgatggcc ggaccttatg
     1741 gcgcggccaa agccgctctt atcaatatga cggaaactat ggctgaagag aacaaaagga
     1801 ataatattca ggtcaacgcc gtcgtacccg gcattataga tacaccgacc aatcgtcagt
     1861 ctatgcctga tgcagatttt agtacgtggg taaaacctca atctatagcc gaagtgatat
     1921 cttttttagt ctctgaaaaa tctaacgcac taacagggtc tgttttgcgt gtaaccggaa
     1981 aattataaac gcctatgaaa tctataaccg atcaacctga atattctttt ggtgaagaat
     2041 tagcccatgc tatcacacat ggtatcggcg ctattctcag tattgccggc ttgacattgc
     2101 ttgtcgcttt ttccgcatta cgcggtgatg catggcatgt cgtatcttcg gcaattttcg
     2161 gaacaacatt ggttcttctg tattcagcat cgacactgta tcatagtttc ccacaaccca
     2221 aggtaaaacg aatttttcgt atcatggacc actcggcgat ttatttattg atcgccggta
     2281 cgtatacccc ttttttactc gtgagtcttc gtggcgcctg gggatggaca ctgtttggtg
     2341 ttgtatggag cgcagccata gccggtgtgt ggtttaaagt gtttcatacc ggaagattcc
     2401 ccaaactttc gacaacatta tacattgtca tgggttgggt cgctctggtt gccgtaaaac
     2461 cgatgatgga aaatattccc ttgggcggtc tgatactcat actggccggc gggcttacct
     2521 ataccgtggg cgtagtattt tatgtcatgc atcgcatgat ctatcaccac gcagtttggc
     2581 atttgtttgt tttaggcggg agtattttgc acttttttgc ggttctttta tatgttattc
     2641 cctaagatcc tctgtttcat ctataatcta ttcgtactat gaaaacccgt tatctcatct
     2701 gtcatctcgt tgcttttttt ttgagttcac atctatcggc tcaaatttca cctttgggaa
     2761 ccgaacaaaa tccgcccggc attgcatgga aagaaattga aacgaaacac tttcgcgttg
     2821 tttttcctga agaaataacc caagatgcac aacgtgtagc cacaacactt gagtatttgt
     2881 atgaaccggt ttcccattca ttaaagaaga aaccgcatag aatcaccgtt attcttcaca
     2941 atcaaaatat tatttcaaac ggatttgtga cgcttgcacc tcgacggagc gaatggtaca
     3001 acacgccacc tcaaactgct tttaccggaa ccaatgattg gtataatctt ctttcggttc
     3061 atgagtttcg tcatgttgtt caatttgata aaatgaatcg cggtcttata gctttgatgt
     3121 atggattatt cggcgaaaca ggaactttgg ccgcaagctt tttgagtgta ccgtcatggt
     3181 attgggaagg tgatgcgaca ggaacggaaa ccgcactaac acaaagcggg cgcggacgcc
     3241 ttccttcgtt taatatgccg atgcgaacta tgttattgcg cggcccacag caaaattatt
     3301 atcagatgct tttgggttcc tacaaaaact atttcggcgg cgcttacgaa atgggctatt
     3361 atatgacgtc gtacgtcaag cgccagcacg gatccgatat gtggtctgat attcttcgcc
     3421 gcaattccaa ttttccgttt ttttatactt tttcctggtc tatgaaagcc aaaacgggga
     3481 acggtgcgcc gaatacattt gaaaatatgt cacaagaact caaagatttg tggtcaactc
     3541 aagagctgtc ttacaaatca gacctgacaa cgatcgaggt tcgctacgac aatggattta
     3601 tgaatccttt cccaaacaat ccaaaaactt tgacggacgg ttctgtcgtt ttttttgatt
     3661 acaatctcgg tgaagccccc ggcataaaaa taaaaacgac agatggagct atttctcata
     3721 ttacgcgatc aacatggtct ggtaatttca gcgcatcaca aaacaaagtt atttgggttg
     3781 accctgattg ggatctgcga tggggattga aaaacggtag ccgcgttgtc gtgtgggatg
     3841 ccactactcg aaatactcaa actcttttaa ctaacacacg actcacctat gttgcgatgt
     3901 ctgccgacgg acaaaaattt gccgctgtgg aacacagccg tagcaataag tcttcaataa
     3961 tattgtttga tacgcaaaca ggtaacatta taaaaaaact ttccaaccct aataacgatg
     4021 ttatcatcaa tccgtcttgg tcttccgaca acaagcatat cgtttatgta cgtcaaaccc
     4081 tgcacgatgg aaaaggtgtt acccttattg atatcgaaaa tgacgccacg accgatgtgc
     4141 tacccgctca acatacgatg aactatacta atcctgtcat ttggaacgac tggatacttt
     4201 tgaatgctca ctttgacggg gtggataata tttttgcctt tcataaatac gaacgcacat
     4261 ggaaacaaat cacgcacagt tcgttaggcg cgttcaatcc gtttgtttcc gctggtaata
     4321 gattagtatt cgatgaatat acagtagacg gttacattgc taattcttta cttttggata
     4381 ccaatcgctg gacaaacgta gatatccagt cccgtaaaaa tgacggttat tatgaaccct
     4441 tgcttcaaca agaacttgga accatccgtt ttgatagcat aacaccgggt accttcgccg
     4501 taaaaaaata caactctcta tccgatctaa tcaacataca ttcatggtcg cctacactga
     4561 atcccagaca atatggtgta tccgtatttt cccaaaacat catgtccacg acacgtttgt
     4621 cggcatctta caattatgac cgcaatgaaa aaacatccgg cggtgaagtg ggcgcatcct
     4681 acggtggctt ttatccgatt gcggatgtta ccgccaaact cggttcgcgt gcgtctacat
     4741 ataccgttac tactatcaaa aacggtaaaa aaactgaaaa actgaagtgg tacggatgga
     4801 atgaaaaaag cttaagtgca gcattgaggt taccactcaa tttttcgagc caaggatggc
     4861 aacgttattt ttcagcatca gccggttttt cgtatacaaa agtttccgac caaaaaatct
     4921 ctttcgcatt tgatcaaaac aatggcgttt tgcaaagtat ctttctttcc ggcttctatt
     4981 ataacgcaac acaaccggcc gcttatgtct atccgcagtg gggtcagcgc atttggataa
     5041 cgtatagcaa agcgcctttt aaagatcatt ataacgggag ccaatttttt gcccaaggtg
     5101 atctgttttt cccgggttta ctaaaacgcc acagcggatg gatacaaggt caatttgaaa
     5161 gacagaaaac cggagccaac tacagatacg caagctggat gatttttccg cgcggttatt
     5221 cgtacattta ccacgatgct attaagaagc ttggggctac ttataccgtt ccgcttatgt
     5281 atcctgatac ggatttttta gccatgtttt atatcaaacg gatttatggg gatgtttttg
     5341 gtgactacgg agtcgggcaa gattattccg gacccaacgt caaaaccaaa tatcggtctg
     5401 ccggggcggg aggatatgcc aacatgcatt tttttacctt accggtcgaa tttagcgtcg
     5461 gttatcgcta tgcatatgcc tttgataaaa agaacactga aaataacaaa catgctcagc
     5521 ctcaaattgt aatcgcgctc acgggacaat tttagtttta ttaacaggaa tacattatcg
     5581 atcatttcgt tctaatctaa gaatcattta aagaggatct atgagttata tagtttggct
     5641 tcatttaatt ttggctgtga attctaacac aaatgatttg gtttggcata aatttgatca
     5701 aggtcttgct ttagcggcta aagaaaacaa agctatactg attgatgttt atacggattg
     5761 gtgcggatgg tgtaaaaaaa tggatcagga tacctattcc gatccgcgtg ttgtaaaata
     5821 cctacgcgaa aattttgtgc tgatacgaat gaaccctgaa acggacggac ctgtgagcta
     5881 cgaaggtgaa caatatccgg cggcggcttt tgcgcaaagt atgcaggtaa cgggctaccc
     5941 ggctacagca tttttcgaat ccaacgggaa aatgatcacg atggtaccgg gatatattca
     6001 atccgatgat tttttgaata ttttaaaata tatggccgaa aaaaaatatt acactgtcag
     6061 ttacgaagat tttttgaaag ccaaatgatg cttaatccgg tttatttttt ttagtttgaa
     6121 ataatccgac aagaaaaata taggttccgg ctataaaaaa gcaccctccg gttgaataca
     6181 ttatatagat cgtatgggga ggacgtatat gtgaaaggtg ttcaaccaca tttgccggat
     6241 tataataaac aacaaacgtc gaatcttctc tcacggaagc tacttcattt cttgctacgt
     6301 taaaccggtt agatttgctc ccaaatccgg gaacttccat atcggtcgta tcagttaccg
     6361 aaaaaccgtt taccgtatat gtataaataa tttccggcct gaaagccctt ttccctacaa
     6421 cttgcgcaga aataacacgt gccggcgtcg gcaaccacgc ccgatacgcc tgatagttat
     6481 ccaattcctt ttgcgcaaaa aacataatac ttgttgccaa caggagaacg acgatcatac
     6541 tgccaagcca tatttttcta ttttttaatt ccaacttacg gccggtaata aaatacagaa
     6601 gcactgataa catcagacaa aacgatactg ttattagtaa attcatcgtc cacctaacaa
     6661 aaactgccgc aatttatgtg caactgcggc agttttaaca tattgaatgt gttgtttatt
     6721 taccctgaaa attggctttt cgtttttcca aaaacgcctt ggttccttcc ttcatatcat
     6781 cgctgctgaa tacaagtccg aataaattgg cctcaaaatt aagaccttca gccaatgtca
     6841 tttccattcc gctttgtacg gcttccagcg tgagtttcac ggctaccggt gctttcgaag
     6901 cgatcgtttg caggattttt tcgcacgtcg gtatcagttc ttcttttttg actatacgat
     6961 ttgccaagcc tatccggaat gcatcttcag ctgaaatcat atcgcctgta caaagaattt
     7021 cgatcgcacg ccctttgccg acaaggcgcg gtaaacgttg cgtaccgcca ttaccggcga
     7081 tgagaccgag attcacttcc ggttgcccaa attttgcatt ttcggaagcg atgcgcatgt
     7141 ggcatgccat cgcaaattca catccgccgc ctagcgcaaa accgttgata gcggcgataa
     7201 ccggtttgtg catccgttcg atgtaacaaa gaaccacttc gcttcggagg gcaaattctt
     7261 tggccgttac cggtgtttgt acggcaagtt cagaaatatc cgcgccggca acaaacgctt
     7321 tttctccgct acccgtaacg atgacgccgc ttacggaggc atcctcattg gcttttttca
     7381 tcgcatccat gatttccatg aatgtttccg tattcaatgc atttagttta tccggcctgt
     7441 taatagtcag ataagcaatc ttgtttttta cttcgaataa aatgtttttg taagacatgg
     7501 ctcaactccg agtgaattta tggtaatgct aatcgaataa ttttttctac taattcctca
     7561 aattccatcc cccccgcttt cgccgctttg ggtacaaggc tcgtggcggt cattccgggc
     7621 aaagtgttta tttctaaaca aaaaagttca ccggattgat tcattctaaa atctacccgt
     7681 gcataacctt tgca
//
LOCUS       DAOQNQ010000147         7640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3604_length_7640_cov_7.582202, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000147 DAOQNQ010000000
VERSION     DAOQNQ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7640
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3604_length_7640_cov_7.582202"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="PK754_09770"
     CDS             <1..1146
                     /locus_tag="PK754_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09835.1"
                     /translation="RYAYCEISIEEVNRQFAERLKKAINVAKTYYMAAKSSEANGDYY
                     IALTQYLEGAKEVVIAEAINKEPIEGDIDGSGKSVSVRATFDTQLKQLLGRMRVEIVS
                     GDAQKGTKNEPLAQPLVARLVYDNMGNVMSVKGAVLVSSSVAPTVVKADLSVQTDGSG
                     NAGFTVHAVESVNASGVNKVRIGLSTSDFEAFATQLPGALEKAKTVFKDFSFSARGSS
                     VSRIAILIFEENIGKPQSNSIIEGDIVKQLLASKYKVIDKNEVYRAISREQAKASAES
                     GDDAAANAIKSMADVLVIGYAKAMESVGGSTNPYGGGTSNRVSAWADCSIRVVDLETG
                     KVLASSDIQREKGLSVGAAEKAGIIALQNLSKKASKEIVDNMNAALK"
     gene            1169..1777
                     /locus_tag="PK754_09775"
     CDS             1169..1777
                     /locus_tag="PK754_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09836.1"
                     /translation="MKSFIIIGCLICSLVACGEVKKKSTEKTEKTPETVQPESESPSS
                     EDKPSSLEELVYEATGASKLRTESAARSNAELNGRTALIETLTEDGVGLLEKFYLENK
                     GMFDASMDLEKYKSDIKAVFKKSTTLKGSTVAEYTQSRKGDTTYAIVEIPLMGGYEAI
                     EEQIVSVGLKNKFLKSEHVAHFKTAFKEFFLNEKKKILTTPA"
     gene            1817..4201
                     /locus_tag="PK754_09780"
     CDS             1817..4201
                     /locus_tag="PK754_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNB09837.1"
                     /translation="MRKWFICLAFVMLVNTAQAQKESDFQSGVVAYSNGQYEQAIGFF
                     ESALKYYSSIKKKKAAAADCYNYLGLLYQVLNQHGKAVENFKAAVPLFIETKNKSGAA
                     SALGNWGISSYRLKLAELRQNKQSFSLEDIGSLMAYHLKAKQYHEELKDKAGVANDLN
                     NIATMYFEGEFYDEASDYLNQALKIHSEIGYKAGMAYDMANLARLYYKTDDETGALKF
                     YQRSVGILEEIGDREGAWRTYSYIGSIYESFMKQAAILKDAQKESENRKSAIVALKKS
                     VEIIESFRGNFTNKDFFDSYLKDKNPVYKRLIRLLKLDDNASEALYYIERSKAKMSND
                     VLSAGKLAFNNKEEQKQVDKVQDLQKKTEELEKKIADEKAKPADKQDKARIDSLSKSL
                     TKNQGDFNALMIDLETKYPNIYQVIKVDPIQLSDIQKQLSDDVVILEYFPADDALYIF
                     MITKDKIEAKSVPVSSTTIDSLVNKYRYYINDVTSLMKRGRFDTKLANWKQGEDSRFY
                     ERHTKPLRDIMVKLNDYLILPVWETIKDAKYKNVTVIPAASLYYVPFQCLATETQDGD
                     ISFLVEKKAVNYLTAATLMDMINKKKQNKINNVLVFGNPDGSLPGAEEEAKIIKAAYT
                     DALLYKNQDATKDKAKDLSGNTEVVHFATHGFLSSDEPQKSFLLMAPNEAKNEDDKLT
                     ISEILKMPLKEKNELVVLSACNTSMGKSATGVELISLSRAFAIAGAPTTVATLWPVDD
                     ESTKIIMVNFYDGLKKGLAKSEAMRQAQIALIRKGDYHIHPFFWAPYVMIGNPK"
     gene            4263..4940
                     /locus_tag="PK754_09785"
     CDS             4263..4940
                     /locus_tag="PK754_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09838.1"
                     /translation="MSNSKKPQTMFGFFSKKAKKTIREDDVIWQAEDIKIHNLILRLA
                     DYSHNSDGIMIIAHFPKTLEQLIVSAKKNNLVLKELYTITDADRWATGYYNDPVAILS
                     KAIFVSSSQPPMVQEEKKKKIIVAEHFPTPEKDILLMNWAINVKAESIQWYESTDNPI
                     FTRFGGDQIRILAEKLGWQQDESMQHRFITSSIGSMQEKIFRKSTGDGDAEDVMQWFK
                     SYVPSSL"
     gene            4961..5467
                     /gene="nbaC"
                     /locus_tag="PK754_09790"
                     /pseudo
     CDS             4961..5467
                     /gene="nbaC"
                     /locus_tag="PK754_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551040.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
     gene            5594..6181
                     /locus_tag="PK754_09795"
     CDS             5594..6181
                     /locus_tag="PK754_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09839.1"
                     /translation="MLLDQVLPDYHVRAQYQILIPCKRDVVWKTLMDMDLTSIQTLRL
                     LMGLRSIPAWLMGKSKPQLKKHTLHDLSDSGMHVISEVTGSEIVLGINGKFWQLSANV
                     RMDIPAERFVSFSENDLAKAAWNFFLSETNGITTVQTETRIFCSDPKMRRKFRLYWTL
                     IGPFSGLIRIALLRKLRSNTIRYEKKNGDKGYEKK"
     gene            6189..7199
                     /locus_tag="PK754_09800"
     CDS             6189..7199
                     /locus_tag="PK754_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNB09840.1"
                     /translation="MKIDIHTHILPEKLPDFAKKFGYGGFVSLEHHKPSCARMIVDGK
                     FFREIESNCWDPHVRIKESAGHGINMQVLSTIPVMFSYWSKPQDGLDVSMFLNDHIAE
                     IVRQFPQRFSGLGTVPLQAPDLAIAELERCMKIGLQGVQIGSHVNEWNLNDERLFPFF
                     QAAEKMGAAIFVHPWDMMGQDRMSKYWLPWLVGMPAETSLAICSMIFGGVFERLPNLR
                     VAFAHGGGSFPSTIGRIEHGFHCRPDLVAVDNKRNPREYLGKFYFDSLVHDARMLRYL
                     IELVGEKRIALGSDYPFPLGEEIPGSLVESMDDLTENIKERILSGTAAEWLNINLETL
                     QK"
     gene            7203..>7640
                     /locus_tag="PK754_09805"
     CDS             7203..>7640
                     /locus_tag="PK754_09805"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNB09841.1"
                     /translation="MQIFINCNDAEYAIDLNSPLDISIPVYFDGLQPNTYGVPVASAR
                     AYEDGKFVGDVRRGGSCNFEEYRIVPHCNGTHTECVGHISKERIAINTYFDRLLIPAT
                     LITIEPEKAVNSAETYAPDKQSADLIITAKSLFYKLHTLPREFM"
BASE COUNT         2501 a         1433 c         1673 g         2033 t
ORIGIN      
        1 cgttacgctt attgtgagat atccattgaa gaagtcaatc gccagtttgc tgaacgattg
       61 aaaaaagcga tcaatgttgc caaaacctat tatatggcgg caaaatcatc ggaagcaaat
      121 ggagattatt atatcgcgct cacacagtat cttgaaggcg caaaagaagt ggtcatagcg
      181 gaggcaatca ataaagaacc catcgaaggt gacattgacg gatccggcaa aagtgtatcc
      241 gttcgagcga catttgacac acaactcaag caattgcttg ggcgtatgcg tgttgaaatc
      301 gtaagcggcg acgctcaaaa gggaacgaaa aatgaacctt tagcccaacc cttagtagct
      361 cgacttgtgt atgacaacat gggcaatgtg atgtctgtca aaggggctgt tttagtttcc
      421 tcatccgtgg ctcctactgt cgtaaaagcg gatttgtccg tacaaacgga tggttccgga
      481 aatgcaggat ttacagtgca cgccgtagaa tcagttaatg cgagtggtgt taataaagtt
      541 cgtatcggcc ttagtacgtc tgattttgaa gcttttgcga cccaacttcc gggtgcgcta
      601 gaaaaagcaa aaaccgtatt caaagatttt agtttttcgg cgcgcggttc atcggtatcc
      661 cgtatagcta ttttgatttt tgaagaaaac attggaaaac ctcaatcaaa ttcaattatc
      721 gaaggcgaca tcgtcaaaca attgcttgca agcaaatata aagtcattga taaaaatgaa
      781 gtatatcgtg ctatttctcg cgaacaagcc aaagcgtcag cagaatctgg cgatgatgcg
      841 gcggccaatg cgatcaaatc tatggccgat gtattggtga tcggttatgc taaagccatg
      901 gagtccgttg gaggttcgac caatccttac ggcggtggta cgagtaatcg tgtatcggcg
      961 tgggctgact gctccatacg tgtagtggac ttggaaaccg gtaaagtttt agcatccagt
     1021 gatattcagc gtgaaaaagg gttgtctgta ggcgcagcag aaaaggcggg gattatcgcg
     1081 ctccaaaatc tgagtaaaaa agcatccaaa gaaatagttg ataacatgaa tgcggcatta
     1141 aaataacgag tgttcatcgg gagaagaaat gaaatcattt ataattatcg gttgtttaat
     1201 atgctcactc gttgcttgtg gcgaagtcaa aaaaaagagc actgaaaaaa cagaaaaaac
     1261 accggaaacc gttcaacccg aaagtgaatc accttcatct gaagacaagc cgtcatcatt
     1321 ggaagaactg gtttatgaag caacaggggc atccaaactc cgtaccgaat cggcggcacg
     1381 cagcaatgcc gaattgaatg ggagaacagc cctaatcgaa acgcttactg aagatggtgt
     1441 agggttatta gaaaagtttt atctcgaaaa caaaggcatg tttgatgcat ctatggattt
     1501 ggaaaaatat aaatcagaca taaaagctgt gtttaaaaaa tcaacaaccc ttaagggaag
     1561 tactgttgcg gaatatacgc aatccagaaa aggggatacg acgtatgcca tcgtcgaaat
     1621 accactgatg ggcggatatg aagccatcga agaacagatc gtatccgtag gattaaaaaa
     1681 taaatttttg aaatcagaac acgtagctca ttttaaaacg gcttttaaag agtttttttt
     1741 gaacgagaaa aagaaaatac taacaactcc ggcatagcgt accggctgaa tatcagagga
     1801 tatcgcgagg atttttatga gaaaatggtt tatttgttta gcgtttgtaa tgcttgtcaa
     1861 tactgcacaa gcccaaaaag aatccgattt tcaaagcggc gttgttgcat attccaatgg
     1921 tcaatacgaa caagccatag gtttttttga atcggcattg aagtattata gcagtataaa
     1981 aaagaaaaaa gccgctgccg ccgattgtta taattatctg gggcttctgt atcaggtgtt
     2041 aaatcaacac ggcaaggccg tcgaaaattt taaagctgcc gtgccattat ttatcgaaac
     2101 aaaaaataaa tccggagcgg cgagcgcttt aggcaactgg ggaatttcat cttatcgcct
     2161 caagcttgcc gagttacgtc aaaacaagca gagtttttct ttagaagaca ttggttcgct
     2221 catggcatat cacctcaaag cgaaacaata tcacgaagaa ttgaaagata aagccggagt
     2281 tgcaaacgat ctcaacaata tcgccaccat gtattttgaa ggtgagtttt acgatgaagc
     2341 atcagactat ctaaatcaag ctttgaaaat tcattctgag atcggttata aagccggtat
     2401 ggcttacgat atggctaacc ttgcccgatt gtattacaaa accgacgatg aaaccggcgc
     2461 attaaagttt tatcaacgca gtgtcgggat tcttgaagaa ataggagatc gcgaaggcgc
     2521 ttggcgcacc tattcatata tcggttcgat ctatgaaagt tttatgaaac aggctgccat
     2581 tttgaaagac gcacaaaaag aatcagagaa tagaaaatca gctatcgttg cgctcaaaaa
     2641 atcggtcgaa atcatagagt ctttccgtgg aaattttacc aataaagatt tttttgacag
     2701 ctatctcaaa gacaagaatc ccgtttacaa acgattaatt cgtttgctta aactggatga
     2761 taatgcgtca gaagcgcttt actacatcga gcgcagcaaa gccaaaatgt ccaatgatgt
     2821 tttgagcgcc gggaaactcg catttaacaa taaagaagaa caaaagcagg ttgataaggt
     2881 tcaagatctg caaaagaaaa ccgaagaact cgaaaagaag attgccgatg aaaaagccaa
     2941 accggcggat aaacaagata aagcgcgcat cgacagtctt agcaagtcat tgaccaaaaa
     3001 tcaaggcgac tttaatgctc tgatgattga tttggaaacg aagtacccca atatttatca
     3061 agtgatcaaa gtagatccga tacagttaag cgatattcaa aagcaactct ccgacgatgt
     3121 agtaatttta gaatattttc cggcggacga cgcgctctat atttttatga tcaccaaaga
     3181 taaaatcgag gccaaatcag ttcccgtatc atccacgacg attgattcac ttgttaacaa
     3241 atatcgctac tatattaacg atgttacttc tttgatgaag cgtggccgtt ttgacaccaa
     3301 attagcaaat tggaaacaag gtgaagactc acgtttttac gaacgtcata ccaaaccgct
     3361 gcgcgacatt atggtaaaat tgaatgacta tttgatttta ccagtttggg aaaccatcaa
     3421 ggacgcaaaa tataaaaacg taaccgtaat tccggctgca agtttgtatt atgttccgtt
     3481 ccagtgtttg gccactgaaa cgcaggatgg cgacatatcc tttttggtag agaaaaaagc
     3541 cgtgaattat cttacggcag caacactgat ggatatgatc aacaagaaaa agcagaacaa
     3601 aatcaataat gttcttgttt tcggaaatcc ggacggaagc ttaccaggag cagaagaaga
     3661 agcgaaaatc atcaaagcgg cttatacgga tgccttacta tacaaaaatc aagatgcaac
     3721 aaaagataaa gccaaagatc tttccggtaa taccgaagtt gtacattttg caacacacgg
     3781 atttttatcc agcgatgaac cgcaaaaatc gtttttattg atggcgccta atgaagcaaa
     3841 aaatgaagac gataaactga ctataagtga aattctcaaa atgcctttaa aagaaaaaaa
     3901 tgaactggtc gttttatcgg catgtaatac ctctatgggt aaaagcgcaa caggtgtaga
     3961 actcataagc ctttcgcgtg cgtttgcgat tgccggtgca ccgacgacgg ttgcaacatt
     4021 gtggccggta gatgatgaat caactaaaat aatcatggtc aatttttatg atggattgaa
     4081 aaaaggattg gccaaatcag aagctatgcg acaagcgcaa atcgcattaa tccgtaaagg
     4141 tgattaccat atccacccgt ttttctgggc accctacgtc atgatcggca accccaaata
     4201 attaaacaca atcaatgata aaaatccccg tttgcatttg caggcggggg gttttattat
     4261 gtatgagcaa ttcaaaaaag ccgcaaacca tgtttgggtt tttctcaaaa aaagcaaaaa
     4321 aaacaatccg agaggatgat gttatttggc aagctgaaga tataaagata cataatctga
     4381 ttttgcgctt ggccgattat tctcataact cagatggtat tatgatcatc gctcactttc
     4441 caaaaacttt agagcaactc atagtatcag ccaaaaaaaa taatttagtt ttaaaagaat
     4501 tgtatacaat aaccgatgcc gatcgttggg cgaccggtta ttataatgat cctgtggcga
     4561 tcttatcaaa agcaatcttt gtgtcttcaa gccaaccacc tatggtgcag gaagaaaaga
     4621 agaaaaaaat cattgtagca gagcattttc ctacaccgga aaaagacatt ttgctaatga
     4681 attgggcgat aaacgtgaaa gcagaaagca ttcaatggta tgaatctacg gacaacccga
     4741 ttttcactcg atttggtggt gatcaaataa gaattttagc cgaaaaattg ggttggcaac
     4801 aagatgaatc gatgcaacat cggtttatta cctcatccat aggttctatg caagaaaaga
     4861 tatttcgcaa atctaccgga gacggggatg ctgaagacgt catgcaatgg tttaaatcgt
     4921 atgttccatc atcgttataa aatattttag gaggtaaatc atgggtcagc tcaccgcttt
     4981 taattttaaa aaatggataa atgagcatcg tcatctcctc aaaccgccag tcggaaatca
     5041 ggtggtttgg aaagacacgg attttataat catggtcgtc ggtggaccta acgcgcgtaa
     5101 ggattaccat tataatgaag gcgaagaatt ctattatcaa ttggaaggta acatcacact
     5161 gaaaattatc gaaaacggta aaccgcgaga tgtcaaaatc aatgaaggag atatttttct
     5221 tctgccacct cgaacccctc attcaccgca aagaccggcg aatacagtcg gtttagtcat
     5281 cgaaaaaaaa cgtgccacgg aagaaaaaga cggatgtata tggttttgtg aaaaatgcgg
     5341 cacgaagctt tatgaggaat atttttatct caccaatatt gtaacacaac ttccgccggt
     5401 atttgatcgg ttttatggaa atccggagca ttgtacatgc aaaaattgtg gtaccaaaat
     5461 ggaacgtccg gtattgcctg tggcaaaaga aaaagcaaaa tctaaaagac aaaaaaccaa
     5521 aaaaaataac gtcataaaga agaaatcaaa aaaacgtatc aaacctgtaa agaggaaata
     5581 atacgaaaat tttatgcttc tggatcaagt tttacctgac tatcatgttc gggcacaata
     5641 tcaaattttg attccatgca aacgcgatgt cgtttggaag acgcttatgg atatggattt
     5701 gaccagtatt caaactcttc gattgcttat ggggttacga agcataccgg cgtggctgat
     5761 gggtaaatca aaaccgcagt tgaaaaaaca tactctgcat gacctgagtg attcgggaat
     5821 gcatgtgatt tcagaagtta ctggctctga aattgtactg ggcataaacg gaaagttttg
     5881 gcaattaagc gccaatgtaa gaatggacat acctgcggaa cgatttgttt cattttcaga
     5941 aaacgatttg gctaaagccg cctggaattt ttttctctca gaaacgaatg gtattacgac
     6001 ggttcagaca gaaacacgaa tattttgtag cgatccaaaa atgcgacgca aattcagatt
     6061 atattggaca ttgatcgggc catttagcgg tttgattcgc attgctcttt tgagaaaact
     6121 tcggtcaaac actattcgtt acgagaaaaa aaatggtgat aaggggtacg aaaaaaaata
     6181 gctatatgat aaaaatagat attcatactc atattctgcc cgagaaactt ccggattttg
     6241 ccaaaaaatt tggatatggc ggttttgtgt ctttggaaca tcacaaaccg tcgtgtgcca
     6301 gaatgatcgt tgacggtaag ttttttcgcg agatagagtc caactgttgg gatccgcatg
     6361 tacgcataaa agagagtgcc gggcatggta taaatatgca ggttttatca accattccgg
     6421 ttatgtttag ttattggtca aaaccgcaag atggtttgga tgtttctatg tttctaaatg
     6481 accatattgc agaaatagtt agacagtttc ctcagcgctt tagtggattg ggtacggttc
     6541 ctttgcaagc gccggatttg gcaatcgctg aattagagcg atgtatgaaa atcgggcttc
     6601 agggcgtaca gatcggatct cacgtcaatg agtggaatct taacgatgaa cggttattcc
     6661 cttttttcca agcagcagaa aaaatgggcg ctgcgatttt tgtacaccca tgggatatga
     6721 tgggtcagga tcggatgtcc aagtattggt tgccgtggct cgttgggatg ccggccgaaa
     6781 cttcgctcgc gatctgttcg atgatttttg gcggcgtttt tgaacgctta cccaatttgc
     6841 gtgtggcatt tgcacacggt ggagggtctt ttccttcgac gatcggacga atcgaacacg
     6901 ggtttcattg tcgtcccgat cttgtagctg tagataacaa acggaatccc cgcgaatatc
     6961 tcgggaaatt ttattttgat tccttagtac atgatgcgcg catgttgcgt tatctgatcg
     7021 aacttgtcgg agaaaaaaga atcgctttgg ggtccgatta tccattcccg ctcggtgaag
     7081 aaattccggg atcattagtc gaatccatgg acgacttaac tgaaaatata aaagaacgaa
     7141 tactaagtgg taccgcagcc gagtggctta atatcaatct tgaaactcta caaaaataaa
     7201 tgatgcagat atttattaat tgtaatgatg cggaatacgc tatagatctt aacagccctt
     7261 tggatatttc aatacccgta tattttgacg gtttgcaacc taatacctat ggtgttccgg
     7321 tcgcatctgc aagagcttat gaagacggga aatttgtagg tgatgtacga agaggcggaa
     7381 gttgcaattt tgaagaatac cgcatcgtcc cgcattgcaa tggtacacat accgaatgtg
     7441 tggggcatat cagtaaagaa cgtattgcaa tcaacaccta ttttgataga cttttgatcc
     7501 ctgcaacact cattacgatc gaaccagaaa aagctgtaaa ttcggccgag acgtacgcac
     7561 cggataagca gagcgcggat cttattatca cagctaaaag tcttttttat aaacttcata
     7621 ctttgcctcg tgaatttatg
//
LOCUS       DAOQNQ010000148         7609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3623_length_7609_cov_6.615436, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000148 DAOQNQ010000000
VERSION     DAOQNQ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7609
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3623_length_7609_cov_6.615436"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2266)
                     /locus_tag="PK754_09810"
     CDS             complement(<1..2266)
                     /locus_tag="PK754_09810"
                     /inference="COORDINATES: protein motif:HMM:NF019229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HNB09842.1"
                     /translation="MKRWLLLWVSTVFVSVTAQQFTKITTGPAVSDGGASRSVNWFDY
                     NNDGYLDLFVSNSSGAKGTSDFLYRNLGVSGNYGFSKIIRGPVVTDFGQSDGASFGDY
                     DNDGDEDVFVVNWYNENNMFYENGGAPYFRYSAVTTGAPVTDGGLSETCVWGDYNNDG
                     YLDLYVTNSGNAGLAQANFLYKNNGNKTFTKITTGSPVTDVAFTRGANWIDYDRDGDQ
                     DLFVVNESGQDNALYKNTLIENGTEGFLKVTGVSIASDGGSSMSASWGDLNNDGNLDV
                     LVVNGIGEHEFLYLQNNDHTFTKVTTGDLVNSGGSSMGSALGDFDNDGDLDVYITNSY
                     AGTPSVDFLFKNMFIENGSLSFQKITTGSIVTDQGDGYGCAWGDYDRDGDLDLFVAKT
                     AGEDQNNALYRNDNTTGYHSVQFRCVGTMSNRSAIGTIIRVKAVIGGQGRWQTRVVEG
                     QSGYCGQSQIAHFGLHNATTIDSVEITWPRGDVDIYTGLSSDSIYVLTENTGYGTLGF
                     SYNTEPIQSQTLRDTTVLEDFGRISFISLTDYFYDPDYTDLKFSVSAITSGITVSLTG
                     DSLICNSVKDFFGSVKIAVRATDDVVTLYDTLTITIEDVAEPILRPAVTVSVFQNPVL
                     SSYADIIVVSTLDLDTAPSLQIQIPGGSGDVSLSLQAISGSARIFKTAYKLQTNGTYT
                     LSAGIRTYQLFDTTISRSFTASLAKVGQTQIAFSPDERSHLTVPAGQSYGLDWILATI
                     EETAGEIVYNYQAKS"
     gene            complement(2411..3145)
                     /locus_tag="PK754_09815"
     CDS             complement(2411..3145)
                     /locus_tag="PK754_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNB09843.1"
                     /translation="MRVLIVDDEMPGRQAIKDLLRNESGMHIIGEAGDGHEAVRQITE
                     QKPDLVFLDIQMPGLDGFGVVRQIGIERMPMTVFVTAYSMHALNAFDVHAMDYVLKPI
                     HPERFAACLKRIRQLKTPNVPAMVGAMTMVQQKYPSQILIRSTGKMDVIKTDDIRWVE
                     AQGDYVQIYTRSQKQLMRSTMSGFETLLDPSVFQRIHRSVIVRVSLIKELQPMFNGDY
                     KIKLDDGTSLSLSRTYHEKVLHCLNG"
     gene            complement(3151..4239)
                     /locus_tag="PK754_09820"
     CDS             complement(3151..4239)
                     /locus_tag="PK754_09820"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNB09844.1"
                     /translation="MNKPDRKFWIIVAAAWLLLGLLYSIQSYHYRVTIGRPGDLLPLL
                     INDIGFFFLWALFSVPVIRWILHWPILRSNWQRRLPVFLMAGLVIAFSQKGLYDAFLL
                     VAYGETTQGFPWERWYRSVIGSFELGFFIYVSMIFVVHTWIYHVNLQREQLDAAALRE
                     RLSQIRLQVLQSQLQPHFLFNTLNTISGLIDVRPADAKLTLGRLGELLRMSLESDQLT
                     EVTLQRELDFSQVYMAIQTTRFGDRLTYKCTVDSAMYGVLVPTMMLQPLLENAVTHGV
                     SKVPGVHMIELKSWRDGGMLCLDINNSFEAIAKPEQAPGLGIGLTNIRERLRQLYGEM
                     AKCELAVHAHGAYVTIRMPWRTSDGNKG"
     gene            complement(4459..6330)
                     /gene="thiC"
                     /locus_tag="PK754_09825"
     CDS             complement(4459..6330)
                     /gene="thiC"
                     /locus_tag="PK754_09825"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNB09845.1"
                     /translation="MSNNNVKIPNAQSKITRDPFPASKKIYVEGSLPGVYVPMREIQI
                     NSRGAHGVKSNGSSTEQVTVYDTSGPYTDPDAQVDIYKGLAGVRSPWIQTREVQALEG
                     ISSQYGRQRENDPSLDAIRFKRTRKVLKAKPGSNVSQMHYAKKGIITPEMEYIAIREN
                     QKRESAIAEYAKQHPGEHFGASIPPVITPEFVRSEVARGRAIIPANINHPEIEPMIIG
                     RNFLVKINANIGNSAVASSIEEEVEKMIWAIRWGADTVMDLSTGKNIHETREWILRNA
                     PVPIGTVPIYQALEKVGGKAEELTWEIYRDTLIEQAEQGVDYFTIHAGVLLRYVPLTA
                     KRVTGIVSRGGSILAKWCLAHHKENFLYTHFEEICEIMKAYDVSFSLGDGLRPGSIAD
                     ANDDAQFGELETLGELTKIAWKHDVQTMIEGPGHIPMHMVQVNMTKQLEVCGEAPFYT
                     LGPLTTDIAPGYDHITSGIGAAMIGWYGCAMLCYVTPKEHLGLPNRDDVKDGVITYKI
                     AAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLSLDPDTAREFHDETLPAEGAKV
                     AHFCSMCGPHFCSMKITQDVRDYAESIKTDETKALEVGMREKAEEFKKTGSKIYVEV"
     gene            6459..7133
                     /locus_tag="PK754_09830"
     CDS             6459..7133
                     /locus_tag="PK754_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNB09846.1"
                     /translation="MAHVTMHTSGEKIPVSKILCVGQNYREHIIELTPAGKEPDFPTE
                     PVIFMKPPSALIYDGGTILRPPVSNDMHHEVELIIVIGKTGKQISPADAMHHVLGYAV
                     GLDMTLRDLQSEAKKKGRPWTIAKGFDTSACISQVMPKEQTGNIMQQDIKLLVNGTIR
                     QNGNTSDMIFDIATIVSYVSQFFTLERGDLIYTGTPKGVGPVVTGDQLHAVLGNDLLT
                     VHAKVG"
     gene            complement(7302..>7609)
                     /locus_tag="PK754_09835"
     CDS             complement(7302..>7609)
                     /locus_tag="PK754_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNB09847.1"
                     /translation="AEHSTAVLVSAKNQMDLAMNIAIGSSIQIALFVAPVLVFASYFF
                     GQPMDLIFTTFEVLAVALSVAVLALVTIDGESNWMEGVQLLAVYVILGIAFYYLPAH"
BASE COUNT         2041 a         1952 c         1651 g         1965 t
ORIGIN      
        1 atgattttgc ctgatagtta tatactattt cgccggccgt ttcttcgatc gtagccaaaa
       61 tccaatccaa accataggat tgccccgcgg gtacagtcag atgtgaacgt tcgtccggtg
      121 aaaatgcaat ctgagtttgg ccgacttttg caagcgatgc tgtaaagctt cggctaatcg
      181 tagtatcaaa caactgatag gtacgaatac ccgccgataa cgtgtacgta ccgttggtct
      241 gcagtttata cgccgttttg aaaatacgcg ccgacccgga aatagcctgc aatgaaagcg
      301 atacgtcgcc ggatccgccg ggtatctgta tttgaagcga tggtgcggta tccagatcta
      361 gtgtgctcac cacgataata tccgcatacg atgacagtac cggattctga aatacggaca
      421 ctgtcacagc cggtcggagt atgggttcgg cgacatcctc gattgttatg gtcagcgtgt
      481 catagagagt tacgacatcg tcggtagcac gtacggcgat tttgaccgaa ccgaaaaaat
      541 cttttacgct attacatatc aggctgtcgc cggttaggct caccgtaatc cccgatgtaa
      601 tcgcggatac cgaaaacttg agatcggtat aatcgggatc gtaaaaataa tccgtgagtg
      661 aaataaaaga tatacgccca aagtcttcta acaccgtagt atcacgaagc gtttgcgatt
      721 gaatcggttc cgtattataa gaaaagccta acgtcccata accggtattt tccgtcaaca
      781 cgtaaatact atccgaagac aagccggtat aaatatccac atccccgcgc ggccaggtaa
      841 tttctaccga atcaattgtc gtcgcattat gcaatccaaa atgggctatc tgtgactgcc
      901 cgcaataacc gctctgacct tcgacgacgc gtgtttgcca ccggccttga ccaccgataa
      961 ccgcttttac gcggatgata gttcctattg cactgcgatt ggacatcgtg ccaacacatc
     1021 gaaattgtac gctgtgataa ccggtcgtat tatcattgcg gtacaacgcg ttattctgat
     1081 cttcgcccgc tgttttggca acaaacaaat ccaaatcacc gtctctatca taatcgcccc
     1141 atgcgcaacc gtagccgtca ccctgatccg ttacgatact gcctgtcgta attttttgaa
     1201 aagaaaggga accgttttcg ataaacatgt ttttgaataa aaaatctacg gatggcgttc
     1261 cggcgtagga attcgtaata tacacatcaa gatcgccgtc attgtcaaag tcgccaagcg
     1321 cactgcccat agatgaacca ccactgttta cgagatcacc ggttgtgact ttggtgaacg
     1381 tatgatcgtt attctgaaga tacaaaaact catgttctcc gatgccgttg acaactaaca
     1441 cgtcaaggtt tccatcattg tttaaatctc cccagctggc gctcatggaa cttcccccat
     1501 ctgaagcaat agacactcct gtcactttca aaaaaccttc cgtaccattt tcaatcaacg
     1561 tatttttata cagcgcgtta tcctgcccgc tctcattaac tacaaataaa tcctgatctc
     1621 cgtcccggtc ataatcaatc cagttagcgc cccgagtaaa tgcgacatct gtaaccggtg
     1681 aacctgtcgt gatttttgta aatgttttat taccgttatt cttgtacaga aaattagcct
     1741 gcgcgagacc ggcattgccg ctgttggtta cgtacaaatc cagatacccg tcattattat
     1801 agtcgcccca tacacatgtc tccgataagc cgccatcggt caccggagcg ccggtcgtaa
     1861 cagctgaata gcggaaatac ggagcgcccc cgttttcata aaacatatta ttttcattgt
     1921 accagttgac cacaaaaaca tcttcatcac cgtcgttgtc atagtcgccg aaactcgccc
     1981 cgtcggattg cccgaagtct gtaaccaccg gaccacggat aattttactg aagccgtaat
     2041 tgcctgaaac accgagattg cgatacaaaa aatccgatgt tcccttagcg ccgctgctgt
     2101 tactgacaaa caagtccagg tacccgtcat tgttataatc aaaccagttt acactgcgcg
     2161 aagcgccgcc gtcgctaacg gccggcccgg tagtaatttt ggtaaattgc tgagccgtga
     2221 ccgatacaaa tactgttgaa acccatagta acaaccatcg tttcatacgc gtctcctatt
     2281 atatctattc atggccaaca tacgcgatga aacggcgccg tcctatccaa acgcgataat
     2341 acgttagaaa aacaggttga accgtgagga ttatgcgatg agaaactgaa aggtcagatt
     2401 ggttcggtga tcaaccgttg aggcagtgta atactttttc gtgataagta cggcttagag
     2461 aaagtgacgt accgtcgtct aatttgattt tataatcacc gttaaacatc ggttggagct
     2521 ctttgatcag actgacccga acaatgacgg atcggtggat acgttgaaaa acggaagggt
     2581 cgagaagcgt ttcaaatcca ctcatcgtgc ttcgcatcag ttgtttttgc gatcgggtat
     2641 agatttgcac atagtcaccc tgcgcttcca cccagcgaat atcgtcagtt tttatcacat
     2701 ccatttttcc ggtggagcga atgagtattt gtgaaggata cttttgttga accatcgtca
     2761 tcgcaccgac catggccggt acattcggcg ttttcagctg gcgaatgcgt ttcaaacacg
     2821 ccgcaaaccg ttccggatgg ataggtttga gcacgtagtc catagcatgc acatcaaagg
     2881 cattaagtgc atgcatgctg taagccgtca caaataccgt catcggcatg cgttcgatac
     2941 cgatctggcg tacaacacca aatccgtcca aaccgggcat ttgaatgtcc aaaaaaacaa
     3001 gatccggttt ttgctcggtt atctgacgca ccgcttcatg gccgtcgccc gcttctccga
     3061 taatgtgcat gcccgattcg ttacgcagga gatctttgat ggcttgacgc ccgggcatct
     3121 cgtcatctac gatgagtacg cgcatagatg ttatcctttg ttcccatcgg aggtgcgcca
     3181 cggcatcctg atcgtaacgt aggcaccatg tgcatgtacc gccagttcac acttagccat
     3241 ctcaccgtac aattgccgga gacgttcacg gatattggta agaccgatgc ccagtcccgg
     3301 tgcttgttcc ggtttggcaa tagcctcaaa gctattattt atatccaaac acaacatgcc
     3361 tccatcacgc catgatttca actcaatcat atggactccg ggaacttttg aaacaccgtg
     3421 cgtcacggca ttttccagca aaggttgtaa catcatcgtc ggcaccaaca ccccatacat
     3481 cgcggaatcg actgtacatt tatatgtcag acgatcgccg aaacgggttg tttgaatagc
     3541 catatacact tgcgaaaaat ctaattcacg ttgaagtgtt acttcggtta actgatccga
     3601 ttccagcgac atacgaagca attcccccaa tcggccaagc gtcagttttg catccgcagg
     3661 acgaacgtca ataagtccgg aaatcgtatt tagcgtatta aaaagaaaat gcggctgcaa
     3721 ttgactttgt aaaacctgca aacggatctg tgataatcgt tcgcgcaaag cggcggcatc
     3781 cagttgctca cgctgtaggt tgacatgata aatccacgta tgcacaacaa agatcatgga
     3841 tacgtaaatg aaaaaaccga gttcaaaaga accgatcacc gagcggtacc aacgttccca
     3901 aggaaaaccc tgtgtcgttt cgccgtaagc aaccagcaaa aaagcatcat acaatccctt
     3961 ttgagaaaag gcgatgacta aaccggccat taaaaaaacg ggtagccgac gctgccagtt
     4021 gctccgaaga atcggccagt gcaaaatcca tcgaatgacg ggaacgctaa acaacgccca
     4081 tagaaaaaaa aagcctatat cgttgataag taacggtagc aaatcaccgg ggcgaccgat
     4141 ggtgacgcga taatggtagg attgtatgct gtagagcaaa cccaaaagca accaagccgc
     4201 ggctactatg atccagaatt tgcgatcggg tttattcatg ggtattaaaa tgatcgtgac
     4261 gacttttgga ataatgccgg tataaattaa cgaccgttag agattttgca acacgtgttg
     4321 ttatactctt aaatctcaac agcatgccta ggaactcggt ttaaactaca aaatttatac
     4381 cgtgattact tcacgcccaa cgtatccttt catacgcatt ttagaagccg ccgtttttta
     4441 ttcaggccgc tagccgtgtt atacttcaac gtatattttg gatccggttt ttttgaattc
     4501 ttcagctttt tcacgcatac cgacttctag cgctttggtt tcatccgtct taatggattc
     4561 agcataatcg cgtacgtcct gagtgatttt catgctgcaa aaatgcgggc cgcacatact
     4621 gcaaaaatgc gccactttgg caccttccgc cggcaacgtc tcatcatgaa attcccgcgc
     4681 ggtatccgga tccagcgaca gattaaactg atcttcccaa cgaaattcaa accgcgcttt
     4741 cgacagcgcg ttatcgcgaa tttgcgctcc gggatggcct ttggccaaat cggcggcatg
     4801 cgcagcgatt ttataagtga tcacgccgtc tttgacgtca tcgcggttgg gcaatcccaa
     4861 atgttccttg ggtgtgacgt aacacagcat ggcgcaaccg taccatccga tcatcgctgc
     4921 accgataccc gacgtgatat gatcgtaacc cggcgcgatg tccgtcgtca gcggccccag
     4981 cgtgtaaaac ggcgcttcac cgcacacttc aagttgtttg gtcatgttga cctgcaccat
     5041 gtgcatcggg atatggcccg gcccttcgat cattgtctgc acatcatgtt tccatgcgat
     5101 tttcgtaagc tcgccaagcg tctccaattc cccgaactgc gcatcgtcat tggcatcggc
     5161 gatggaaccc ggacgcaaac cgtcacccaa actaaaactc acgtcatagg ccttcatgat
     5221 ctcacagatt tcttcgaaat gcgtgtacaa aaaattttct ttatgatgcg cgagacacca
     5281 cttagccaat atcgaaccgc cgcgtgacac gatacccgtc acacgtttgg ctgtgagggg
     5341 tacataccgc aataaaacac cggcatgaat cgtaaaataa tccacgcctt gttcggcttg
     5401 ttcgatcagc gtgtcgcggt aaatctccca ggtaagttcc tccgctttac cgccgacttt
     5461 ttcgagcgcc tgatagatcg gcacggtacc gataggtacg ggtgcattcc ggagtatcca
     5521 ttcgcgcgtt tcatgtatat tttttccggt ggaaagatcc atcaccgtat ccgcacccca
     5581 acggatcgcc caaatcattt tttccacttc ttcttcaatg ctcgaggcca cagccgaatt
     5641 accgatattg gcattgatct tgaccaaaaa attacgacct atgatcatag gttcgatctc
     5701 ggggtgattg atattggccg gaataatcgc ccgtccgcgc gctacttcgc tgcgcacaaa
     5761 ctccggggta atcaccggcg gaatcgaagc gccaaaatgt tcgccgggat gttgttttgc
     5821 atattccgct atcgccgact cccgtttttg attttcacga atagcgatgt attccatttc
     5881 cggagtgatg atgccttttt tcgcgtaatg catttgcgat acattgcttc cgggtttggc
     5941 tttgagcact ttgcgtgtac gtttgaaacg aatggcatcc agcgatggat cgttttcacg
     6001 ttgacgcccg tactgtgagg aaatcccctc caatgcctgc acttcgcgcg tctgtatcca
     6061 cggggaacgc acgccggcca aacctttata gatatccacc tgtgcatccg gatcggtgta
     6121 cggtccgctg gtatcatata cggtcacctg ttcggttgaa ctcccgttac tcttcacacc
     6181 atgcgcgccg cggctattga tctgtatttc gcgcatcggc acgtacacac ccggtaaact
     6241 tccttcgaca tagatttttt tggaagccgg aaatggatcg cgtgttattt tgctctgggc
     6301 gttggggatt ttgacattgt tattggacat aagttctcct gtatgtacgg aaaatttttt
     6361 caatatttag acagacggtg cgattagaaa ttatctcttc ccgatcgcgc aaaaacttac
     6421 taagttattg cacacaattc aagcccaagg aaaatatcat ggctcatgtc actatgcaca
     6481 catcgggaga aaaaataccc gtttcaaaaa ttttatgtgt cggccaaaac taccgcgaac
     6541 acatcatcga attgactccg gcaggcaaag aacccgattt tcctaccgaa ccggtgattt
     6601 ttatgaaacc gccttccgca ctgatatacg acggcggtac gatcctacgt ccgcccgttt
     6661 ccaacgatat gcatcatgag gtcgaactca tcatcgtgat cggaaaaacc ggaaaacaaa
     6721 tatctccggc cgatgcaatg catcacgtac tcggttacgc tgtcggacta gacatgacat
     6781 tgcgcgatct gcaaagtgaa gccaagaaaa aaggacggcc ctggacgatc gctaaaggct
     6841 ttgacacctc ggcgtgcatt tctcaggtta tgcctaaaga acagaccgga aatatcatgc
     6901 agcaagatat caaacttctt gtcaacggta cgatccgtca aaacggcaat acatcggata
     6961 tgatatttga tatcgcgact atcgtaagtt acgtttcaca attttttaca ctggagcgcg
     7021 gcgatctgat ctatacggga acgccgaaag gcgtcggtcc ggtcgtgaca ggcgatcaac
     7081 ttcacgccgt actcggaaat gatttgttga ccgtacacgc gaaagtggga taatccaccg
     7141 ctgataaatt ttctaagagt ctgtcatttc tacgtcgttg cgtaatcccg tcgaaatgac
     7201 cgataactgt tttagcggcg atgcgtaaaa aaaataagga cgtctcggtg tatcgtgtta
     7261 aacacaacgc tcgacgtcct ccaaaattat cctaggtttt tttaatgcgc aggcaaataa
     7321 taaaacgcga tacctaatat cacatacacc gccaaaagtt gaacgccttc catccagttg
     7381 gactcaccgt ctattgtcac caaagccagt acggcaaccg ataacgctac cgccagaact
     7441 tcgaatgtcg taaaaatcaa atccatcggt tgaccgaaaa aatagctcgc aaacaccagt
     7501 accggggcaa cgaataacgc aatctgaata cttgatccga tagcaatatt catcgcaaga
     7561 tccatttgat ttttagccga cactaaaaca gccgtactgt gctctgccg
//
LOCUS       DAOQNQ010000149         7581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3643_length_7581_cov_5.584906, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000149 DAOQNQ010000000
VERSION     DAOQNQ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7581
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3643_length_7581_cov_5.584906"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="PK754_09840"
     CDS             <1..1287
                     /locus_tag="PK754_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09848.1"
                     /translation="LRIISKDPDCYAELFSGDNKLLIGDRYMWTLDGKFLGAFDRLGG
                     FDLVMSPNKKYFTGGAWLWNMDKMIVADMENKKLLHPAYSHDGCYLAGVENYSFRIMD
                     SQYRIIYEYTMPRKKQPGKKNEYVTYTATAFSPDSKYLTLGMSDGTIQIWEMPTSLIM
                     SHNGGIVSLAVCDDGTVISSGGEGNLRWWSKGNFIKQITQVYATDIAVSDKQHIATAG
                     RNGAIHIYDKQGQELTRWGAHTGEVTNVEYMPATDEWISAGSDGAVFAWTSGGKKTRT
                     ILPESGDPILSLSVENTTGQIIVSTASGSVYGGGSHEPMKKILTQPVWIPAVQWSRED
                     SYYFSTRTNEVGRAFLGGQADQTKLKGAENIHVFVKAGDRIFAGDTDGNVWRMNGLER
                     EIVYTGSTAVTALSASPDGRVVYAGFMDGLIFKLQK"
     gene            complement(1375..1836)
                     /locus_tag="PK754_09845"
     CDS             complement(1375..1836)
                     /locus_tag="PK754_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNB09849.1"
                     /translation="MNVVDSELFKKLGEDMKAAMKSGEKEKLDIIRTLKAGLQKVLID
                     KGQNFKAEDEMNFLLAESKRRKEAIELYEKGGRQDLADKEKTELALISEFLPKQLNDD
                     ELAAIIDEAIRTTGITSAKDMGKLMGALMPKVKGKADGKKVQDMVKSRLAG"
     gene            complement(1860..5120)
                     /locus_tag="PK754_09850"
     CDS             complement(1860..5120)
                     /locus_tag="PK754_09850"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB09850.1"
                     /translation="MNRKSLYLILFCWIMTAMPMHAQQPFLRYYNLSSGFPHAQVWDI
                     VQDANGILWFATTGGLVRYDGIEYTVYTKDEGLPGNTIRNLSLDSSGIIWLGTDEGLV
                     RFDGKNFTTITISDNGESLAVWQLIKDRTGRTWITTDRGIYLQDTAPYHHIKISHLPE
                     SASFRTAFVDSYGHIWFAGSRGIAWKYFNGTTYTDSFFVIPRSPSAFPSYVSEMAQDA
                     RGRLLFATDNGLGVLDINAMLDQKSLDVRAFTRLYDSRNGLRSSKIKSVCAANDASVW
                     IGTELGFAKFTNGNFENIFADENVSSNSCVSIFSDREGIIWVGTDGGGVIKLPFHNIR
                     NFTVKDGLAFNVVNAITGDGKGTIFIGSDRGVNRWDGKKMHLITPNNPLANTIWSLHV
                     DRKGVLWIGSEQHIYTYEQGQLTDRTDIFTKTGGPIVDIEEDLTGQLWFGTLSGLIRW
                     DGLSATVINKDSGLAGNAVWSVFADSQNTIWAGIDGGLSRIEQLPDHTNHITNWTDRD
                     GLIDNTVNVVRRDPSGIVWVGTDAGISRFDGSHFINYKPKSLGFGDNVVPVLAWDFMH
                     NELWVGCKGFGRVRVDAGMQVTKMLNRNRGLPIDESTTNNAFFIDEKHQIWIGTFGGV
                     TCYDEKSAPMNMPAPHVLIQRIRSDDSVFVLPDFTPHLNDNSWTIETNTVMFEFVAPS
                     FLDEKDNWYQYRLEGFDQTWSEWTRKSEVRYTNLGPGTYTLHVRARNAEYVLSSDEAR
                     ATLHIPRPFWMNPVIMLIFTGIALYGAYGTYRIRVAFKLRSMRDRTQRLESMVNQRTS
                     EIERQKNELEGLLDQLKRTQSQLVQSEKMSALGQLVAGVAHEINNPTSVLTGNVAYVG
                     DYIKVLRQLIAKYESLLPASREIASYKEALDYEFVISDLDNLLSAIQHASDRIRLIVK
                     DLRNFSRLDEADLNQVDIHECIETTLKLFYNQYRHILRIEGQYEATKAVYCYANQINQ
                     VLLNLFINAAQAIEQRFRGKKTIEGVIRIHTANTENGIQVKIRDNGAGIPPSIRSKIF
                     DPFFTTKPVGQGTGLGLSISYSIIEKHNGSISVDSREGEFTEMTFSLPLQPKTLPS"
     gene            5304..6242
                     /locus_tag="PK754_09855"
     CDS             5304..6242
                     /locus_tag="PK754_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013663838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HNB09851.1"
                     /translation="MQTFAQEAKLRYAIAIHGGAGVISKDMPDSVKNKYIAGLTEALQ
                     KGVAMLEAGRNGLDVVETVVRALEDNPLFNAGRGSVFTADGTHEMDASIMNGKSLACG
                     AVAGVKTIKNPISLARLVMEKTSHVFLSGEGAEVFAREMGVDTAGADYFYDAYRYEQW
                     KKAKQKHQIRLDHSADEKKGTVGCVVLDNEGNLYAATSTGGMTNKRKGRIGDSPIIGA
                     GTYASNASCAVSCTGTGEQYIRHTIARDLAARVEYKNETLSQSARYLIHEVLEKGDGG
                     LIAVDKKGHIVLEFNTPGMFRGAADASGRFEVKIWE"
     gene            6317..>7581
                     /locus_tag="PK754_09860"
     CDS             6317..>7581
                     /locus_tag="PK754_09860"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNB09852.1"
                     /translation="MEKVISHIESNKDRYVEELKDFLKIPSISTNPENKKDVQHCAEW
                     VAQHMTHIGLNNVKVCPTAGHPVVYGEWLGAPGKPTVLLYGHYDVQPVDPLNLWTSGP
                     FEPTIRDGYIYARGSADDKGQVFLNLKSIEAHLKINGSLPVNVKLMIEGEEEIGSEHL
                     EPFMKENKEMLKADVVLISDTSMFDDGIPSIAYGLRGLCYMQVELTGPNRDLHSGTYG
                     GAVGNPINELANMIAKLKDKNGKITIPGFYDKVKPLTKAQRDAYKKLPFNKKGYLKDL
                     GIKETFGEKGYSILEQVSGRPTLDCNGIWGGFQGEGAKTVLPSKAGAKISMRLVPGQK
                     PAEIAKLFEKYIKKIAPKTMQVKVIALHGGDPAMTDITTKEMTAASTAMQKVFKKKPL
                     FTREGGSIPVVAGFERLLGLKTIMLGFGL"
BASE COUNT         2063 a         1659 c         1882 g         1977 t
ORIGIN      
        1 ttgcgcatta tttccaaaga tccggattgt tatgccgagc ttttttccgg agacaataag
       61 ttgctaatcg gcgaccgtta tatgtggacg ctggacggaa aatttttagg cgcatttgat
      121 cgtttgggtg gatttgactt ggtcatgtca cccaataaaa aatatttcac cggtggtgct
      181 tggctttgga atatggataa aatgatcgta gccgatatgg aaaataaaaa gttactgcat
      241 cctgcgtata gtcatgacgg ctgttatctt gccggtgttg aaaattattc cttccgtatc
      301 atggattccc aataccgcat aatctatgaa tacaccatgc cgcgaaagaa acaacccggg
      361 aaaaaaaatg aatacgtgac atacacggca actgcgtttt caccggattc gaaatattta
      421 actctaggta tgtcggacgg gacgatacaa atatgggaaa tgccaacgtc tctgataatg
      481 agccacaatg gcgggattgt atcccttgct gtttgtgatg acggcacggt tatttccagt
      541 ggcggagagg gaaatttgcg gtggtggagt aagggtaatt ttatcaaaca aattacgcaa
      601 gtatatgcga ccgatattgc cgtcagcgat aagcaacata tcgccacggc ggggagaaac
      661 ggggctattc atatctatga caaacaaggt caagagctaa cgcgttgggg ggcgcatacg
      721 ggtgaagtga ccaacgtcga atatatgccg gctaccgatg aatggatttc cgccggttcg
      781 gatggagctg tctttgcctg gacatcagga gggaaaaaaa cgcgtacgat tttaccggag
      841 tccggagatc cgatcctatc gctgagtgtc gaaaatacta ccggccaaat catagtttcg
      901 acggcttccg gttctgtgta tggcgggggt tcgcacgaac ctatgaagaa gatattaacg
      961 caacctgtgt ggattcctgc ggtgcagtgg agccgagaag attcgtatta tttttctaca
     1021 cgtaccaatg aagtcggtcg cgccttctta ggaggtcaag ccgatcaaac aaaattaaaa
     1081 ggcgctgaaa atatccatgt ttttgtcaaa gcaggggatc gcatcttcgc cggagatacc
     1141 gacggtaatg tgtggcgtat gaacgggctt gaacgagaaa tcgtttatac aggatcaacg
     1201 gcggtgacgg ccttgagtgc atcaccggat ggccgtgtag tttatgccgg ttttatggat
     1261 ggtttaatat ttaaattgca gaaatagttt tcgtcaagtg acgtgatgga tgtaataaaa
     1321 aacgcctcca tgttgaggcg tttttttatt gactatgatt ttatgaggaa tgcctcaacc
     1381 ggctaatcgg gatttgacca tatcctgaac tttttttccg tcggctttac ctttgacttt
     1441 aggcataagc gcacccatga gtttacccat gtctttggcg gaggtaatac ctgttgtacg
     1501 aatcgcctcg tcaataatag cggctaattc atcatcgttc aactgtttcg gaaggaactc
     1561 gctaatgagg gccagttcag ttttttcttt gtcggccaga tcctgacgtc cgcctttttc
     1621 atataattcg atagcttctt tacggcgttt gctttccgcg agaagaaaat tcatctcgtc
     1681 ttccgcttta aagttttgac ctttatcgat caaaactttc tgcagcccgg ctttgagcgt
     1741 gcgaataata tctagttttt ccttttcgcc ggatttcatg gcggctttca tatcttcgcc
     1801 gagtttttta aataattcgg aatcaacaac gttcataaca ctccttgtat gtttataaat
     1861 tatgatggta gagttttagg ttgtaaagga agactaaatg tcatctcggt aaattcacct
     1921 tcgcggctgt ccacggagat ggagccgtta tgtttttcga tgatggagta ggatatggac
     1981 aaaccgagac ctgtaccttg tcctacgggt ttggttgtaa aaaaaggatc gaaaatctta
     2041 ctgcgaattg acgggggaat gcccgcgcca ttatctctga ttttaacctg gataccgttt
     2101 tctgtattgg ctgtatgaat tcgtatgaca ccttcgatgg tttttttgcc gcggaatcgt
     2161 tgttcgatag cttgtgccgc attgataaaa agattgagca aaacttggtt gatctgattg
     2221 gcataacaat aaaccgcctt ggttgcttcg tattgtcctt caatacggag aatgtgacga
     2281 tattggttat aaaaaagttt cagtgtggtt tcgatgcatt cgtggatatc cacctgattc
     2341 agatcggctt catcaaggcg tgaaaaatta cgaagatctt tgacgataag gcggattcgg
     2401 tcggaggcgt gttgtatggc ggataacaga ttatccaaat cgctgataac aaattcgtaa
     2461 tccagtgctt ctttatacga tgctatttct cgggatgccg gtagcaagga ttcgtatttg
     2521 gctatgagct ggcgaagtac tttgatataa tctccgacgt aagccacgtt gcccgtcaga
     2581 acggacgtgg ggttgttgat ttcatgtgcg acaccggcga cgagttggcc gagcgccgac
     2641 attttttctg actgcacaag ttgcgattgg gttcgtttga gctgatccaa taagccttcg
     2701 agttcgtttt tttgccgttc gatctccgag gtgcgttgat tgaccatgga ttcgagacgc
     2761 tgggtacggt cacgcatcga gcgcagtttg aatgcgacgc gtatgcggta tgtcccgtaa
     2821 gcgccataca gagcgatgcc tgtaaaaata agcatgatga cgggattcat ccaaaatggt
     2881 cgcggaatgt gaagcgtagc gcgcgcttcg tccgatgaca acacgtactc ggcattacgt
     2941 gcacgaacat ggagcgtgta agtgccgggc cccaaattgg tataacgtac ttcgctttta
     3001 cgtgtccatt ccgaccacgt ttggtcaaat ccctcgaggc gatactgata ccagttgtct
     3061 ttttcatcaa gaaacgacgg tgcgacaaac tcaaacataa cggtattggt ttcgatcgtc
     3121 caggagttat cgtttagatg cggcgtaaaa tccggcaaaa caaaaacgga atcatccgat
     3181 cgaatccgct gaatcagaac gtgcggtgcg ggcatattca tgggcgcgga cttttcatca
     3241 taacaggtga caccgccgaa tgtaccaatc catatctgat gcttttcatc aataaaaaaa
     3301 gcattgttcg tcgtgctctc gtctatcggt aaaccgcgat tgcggttgag cattttggta
     3361 acctgcatcc cggcatccac acgcactcga ccaaaacctt tgcagcccac ccacaattcg
     3421 ttatgcataa aatcccaggc gagtaccggt acgacgttat ccccaaaacc gagagacttg
     3481 ggtttgtaat taataaaatg actaccgtca aaacgtgaaa ttccggcatc cgtacctacc
     3541 catacgatac ctgaagggtc acggcgaacc acattgactg tattgtcaat cagtccgtcg
     3601 cgatctgtcc agtttgtgat atggttcgta tggtcgggca attgttcaat acgcgaaagc
     3661 cctccgtcaa ttcctgccca aatggtattt tgagaatcgg caaaaacaga ccacaccgca
     3721 ttaccggcga gtccggagtc tttattgatc accgttgcgg ataggccgtc ccatcggatc
     3781 aaacccgaga gtgtgccaaa ccaaagttga ccggtgaggt cttcttcgat gtctacgata
     3841 gggccgccgg ttttagtaaa tatatcggtt cgatcggtga gttgtccctg ttcataggtg
     3901 taaatgtgct gttcggagcc aatccataaa acacctttgc gatcaacatg gagagaccaa
     3961 atcgtattag ccaacggatt gtttggtgtg atcaggtgca ttttttttcc atcccagcga
     4021 ttgacgcccc gatcgctgcc gatgaagata gttcctttcc catcgccggt gatcgcattt
     4081 accacattaa aagctaatcc gtctttgacg gtgaaattgc gaatattgtg aaaaggtaat
     4141 ttgatcacgc cgccgccgtc tgtgcccacc caaataatgc cctcgcgatc ggaaaaaatt
     4201 gaaacacaac tattagatga tacattctca tcggcgaata tattttcaaa attaccgttt
     4261 gtaaacttgg cgaaacctaa ctcggtgccg atccacacgg acgcatcatt ggccgcgcat
     4321 acggatttta ttttgctgct tcgaagtccg tttcggctat catataatcg tgtaaaagcg
     4381 cgcacatcaa ggctcttctg gtcaagcatc gcattgatat ccaatacacc gagtccgttg
     4441 tcggtggcaa aaagtaagcg cccgcgggcg tcctgagcca tttcagaaac ataggacgga
     4501 aatgcggatg gtgatcgcgg tataacaaaa aaagaatcgg tgtaggttgt accgttaaaa
     4561 tatttccaag cgataccccg actaccggca aaccagatat gaccatagct gtctacaaaa
     4621 gccgtgcgaa aagacgcgga ctcaggaaga tgactgattt tgatgtgatg gtatggagcg
     4681 gtatcttgca aataaatgcc gcggtcggta gttatccacg tgcggcccgt gcggtctttg
     4741 atgagttgcc aaactgctaa agattcgcca ttgtcggaga tggtgatcgt agtaaaattt
     4801 ttaccatcaa aacgaactaa gccttcatcc gttccaagcc agatgatacc ggacgaatcc
     4861 aaactcagat ttcgaatcgt attgccgggt aatccttcat ctttggtata taccgtgtac
     4921 tcgatgccgt cgtagcgtac taatccgccg gtcgttgcga accacaggat gccattggca
     4981 tcttgcacaa tatcccaaac ttgcgcatgt ggaaaaccgg aactcaggtt ataataacga
     5041 agaaagggct gctgcgcatg catcggcata gccgtcataa tccaacagaa taaaatcaag
     5101 tacagcgatt ttcgattcat gccgcgtagg atattgtgaa taaatgtcgg aaaatatagg
     5161 cgtccaacgt gcgaaatgca aaaacaaaac atgtataaaa agttgaatct cacgggtaaa
     5221 aatggtaaca tcccacgtaa aatcagacga tatttttcgt aagttttatg cacctatttc
     5281 ttctaatttt tttagccgtt accatgcaaa cctttgcgca agaggccaaa ctacgatatg
     5341 ccatagccat tcacggcggt gcgggggtga tttccaaaga tatgcccgac tctgttaaaa
     5401 acaaatacat agccggtttg acggaggcgt tgcaaaaagg cgtggcgatg ttggaagccg
     5461 gacggaacgg gctggatgtc gtcgaaacgg ttgttcgcgc tctggaagac aatccgttgt
     5521 ttaatgcagg ccgaggttcg gtttttactg cggatggtac gcatgagatg gacgcttcga
     5581 tcatgaacgg aaaatcgtta gcatgcgggg cggtagccgg tgttaaaaca ataaagaatc
     5641 cgatttcgtt ggcacgtctc gtcatggaaa aaacatcgca tgtgttttta tccggtgagg
     5701 gtgctgaagt ttttgcccgt gaaatgggtg tggatacggc cggtgcagat tatttctacg
     5761 atgcctaccg ctacgagcag tggaaaaaag cgaaacagaa acatcagata cgacttgatc
     5821 attcggccga tgaaaaaaag ggtacagtag gttgcgtcgt gctggacaac gagggaaatt
     5881 tatatgccgc cacttcgacc ggcggtatga ccaataaacg gaaaggccgg attggggatt
     5941 cgcccatcat cggcgccggt acttacgcca gtaatgcctc ttgcgctgtg tcgtgtaccg
     6001 gtacgggtga acagtatatt cgccacacga tcgcccgcga tctggctgcg cgtgtcgagt
     6061 ataaaaatga aacgttgtct cagagtgcgc gttacctgat ccatgaagtt ttggaaaaag
     6121 gcgatggcgg attgatcgct gtggataaaa aaggccatat cgtgcttgag tttaatacgc
     6181 cgggcatgtt tcgcggtgca gccgatgcat cgggaagatt tgaagttaaa atctgggaat
     6241 aagacgaaac gaaaaaaaag ttctgttcag tataaattaa ccttaattac ctatatcatt
     6301 tcgaaccgga gacaacatgg aaaaggtcat cagccacatc gaatccaaca aggaccgtta
     6361 tgtcgaagaa ctcaaagatt ttttgaaaat tcccagcatc agtaccaatc ccgaaaacaa
     6421 aaaagatgta cagcattgtg cggaatgggt agcgcagcac atgacccata tcggattgaa
     6481 taacgtaaaa gtttgcccta cggcggggca tcctgtcgtg tatggcgaat ggcttggcgc
     6541 accgggcaaa ccgaccgtac ttttgtacgg gcactacgat gtgcagccgg tcgatccgct
     6601 caatctttgg accagcggtc cttttgaacc gaccattcgt gacggctata tctatgcccg
     6661 cggatcggcg gatgacaaag gccaggtgtt cctcaatctg aaaagcatcg aagcgcattt
     6721 gaaaatcaac ggtagtctgc cggtaaacgt caaactgatg atcgaaggcg aagaagagat
     6781 cggcagcgaa catcttgagc cgtttatgaa agaaaacaag gaaatgctca aggctgatgt
     6841 cgtattgatc tccgatacgt ccatgtttga tgacggtatt ccctcgattg cttacggatt
     6901 gcgcgggctc tgctacatgc aagtcgaact gaccgggccg aaccgtgatc tgcacagtgg
     6961 tacttacggt ggcgccgtcg gtaatccgat caatgaattg gctaacatga tcgccaaact
     7021 caaagacaaa aacggaaaaa tcacgattcc gggattttac gataaagtca aaccgctcac
     7081 caaagcacaa cgcgatgctt ataaaaaact gccctttaat aaaaaaggct atttgaaaga
     7141 cctcggcatc aaagaaacat tcggtgaaaa agggtattcg attttagagc aggtgagcgg
     7201 gcgtccgaca ctggattgca acggtatctg gggcggtttc caaggtgaag gcgccaaaac
     7261 cgtattgccc tccaaagccg gtgcgaagat cagcatgcgg ttagttcccg gtcagaaacc
     7321 ggcggagatc gccaaacttt tcgaaaaata tatcaaaaag atagcgccga aaacgatgca
     7381 ggtcaaagtg atcgcgttgc acggcggtga tccggctatg accgatatta cgaccaaaga
     7441 aatgaccgcc gcttcgacgg cgatgcaaaa agtattcaaa aagaaaccac tttttacacg
     7501 cgaaggcggt tcgatccctg tcgtggcggg ttttgaacgc ttgctgggac tcaaaacgat
     7561 tatgttggga tttggtttgg a
//
LOCUS       DAOQNQ010000150         7569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3657_length_7569_cov_5.622837, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000150 DAOQNQ010000000
VERSION     DAOQNQ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7569
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3657_length_7569_cov_5.622837"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PK754_09865"
     CDS             complement(<1..186)
                     /locus_tag="PK754_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09853.1"
                     /translation="MRRCWFLLCICTGGWLAAQDSLLTLEEAIRIAEKNNALLAVARW
                     DMRKADQQVREAYGYAMP"
     gene            complement(400..3747)
                     /locus_tag="PK754_09870"
     CDS             complement(400..3747)
                     /locus_tag="PK754_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNB09854.1"
                     /translation="MHHTRYLLLKVMLFLLFTSFANAQHNVRIVIPSHHTLGVTCFWM
                     TEDGRYAVTGSKDNTLKLWDIHRGREIYSRKGFTNFNSDGVLNFPMSVHISSDQRLAA
                     WGDNYGNVSIWDLDSDSIVHVIMLGRSQMGGNLSIRGLQFGSDNRTVLVIAEYAVKMY
                     DAMSGAEIGIFCRSEEKITRIAVHGQTVLTGDRKGTVRMWDFSTQSEIKVLRDGNNQV
                     LGQITDICISNDGLLAAAGTYDKKLFVFDLNEKKTKSEINIHKKHITALCFAERNQLM
                     SASYDGLIFKTDIVNGITTPLTDKDLTSKLGAPTVLGYSANKKILLCGTEWNTSMVLL
                     HMDGDSSTAMLGRKTEPAMAIFTDPDEERIIVGYKSFVSVWSLTYGKPQYNYAISPVR
                     HYAFNPNRRLLAAAIGDTIHILNIVSNSVINQFRTSDEDKTDFINALRFSHDGQTLAV
                     GGLTGYDRPNLKIFDLTNPNKYFGANMDTHSVHQIQFFKDGSLMIGGYNKCPIWWNWQ
                     TKKIKKLYAGPGSINYSIALSDDEKTAFFGFYIKNYLMDVNTGRFRQSFVMNGTVRSA
                     LLPSDNKSVLGACYNESDPLEGIMEWDLDSGKKIGAYRGHFGQVNQLNFINKEQFFLS
                     AGNDGSVRLWNRQSKKEIAKFIGFDQGNGWVTVVDNGYYMATKNITDHIYYSSGKKIY
                     NFDQFDLQYNRPDIVLERLGKAPPELIRTYKNAYLKRLQKMGFDPVNFESERSFNVPE
                     ITIPDLKTDLIETQNDTYTFSVHLKDKLYALDRLFVEVNGVPLYGVRGKSIKAENMLN
                     SHQTIKVTLSEGRNIIRASVLNTRGVESVSASLEVYYRPHVPTKPVLYVVAIGVSQFI
                     DSNMNLTYAAKDAEDMASLFSVNKDAYADVVIQTLTNSQATRNNIVKTKNLLMKSTVN
                     DEVLLFVASHGLLDDQLNYYIATHDVDFNRPSFNGLSYDELESLTEGIAARKKLMLID
                     ACHSGEVDKEETVLTNVAVHQESKGVSSRGFKTLQNKNSVGLINSFELMKALFADLRR
                     GTGTIVISSAAGKEYAFESEAWKNGVFTYSLIEGLKSKNADTNQDRSITVSELRDYVT
                     RRVSDLTQGKQNPTSRSENLESDFKIW"
     gene            complement(4081..4965)
                     /locus_tag="PK754_09875"
     CDS             complement(4081..4965)
                     /locus_tag="PK754_09875"
                     /inference="COORDINATES: protein motif:HMM:NF023329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3421 domain-containing protein"
                     /protein_id="HNB09855.1"
                     /translation="MRFNRFSLLLVCAALFMWLHVAVNGYAQGAKGGSNAVKKTVTGM
                     TPSTESLTKWVPFTGVIPENAVVGGYENGQPMYVGRVAFEGGMHPGKVIGGGYCNIGW
                     GGKEYSYDKDFEILTAPANAVTWVAYKGTTPANAILGGYNDAAKKEPLYIAKHAYKDG
                     EHCGKLWANACNIGWGGAEVVLKNSIFVLAAKEEVKKPTGPYVRTENSHYLIGQIIPV
                     EYGNFPGSPSVWITVVPKGKKDTERGEYVYAIEKDGVVEINSVTLGEGEYELRAYFGA
                     FDTAAKARATIIMQGGGQ"
     gene            5459..6577
                     /locus_tag="PK754_09880"
     CDS             5459..6577
                     /locus_tag="PK754_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNB09856.1"
                     /translation="MKFSKLLPLVMAFITISFSISYGQTWQPAGGGSKKASVSELIGL
                     TNVTVEYYRPAVNGREGKIWGNIVHYGFADLGFGTTKAAPWRAGANDNTTIEFTTPVF
                     IEGKPLPAGKYGFFIAMSETKATLIFSKYNTAWGSFYYDPKDDALRVDVPVIKTAESV
                     ERLKYEFSDQTDDSAVLSLLWEKVKIPFKVSIDAKKEQIEVFRKAFNSAKFYVYWQNM
                     HAAANYCLVNNINLEEGMGWSERSINDFFGEANFSTLSTYAGFLEKFNRKKEADSVMQ
                     KAFPLGTALQIYSYARSLTKMKENQKAYEVFKIGYDKYPNDPNIILGMARGNAAIGNI
                     NEALNFCEKALTLFADPSSTGIIKKMMEDLKAGKDINK"
     gene            complement(6685..7296)
                     /locus_tag="PK754_09885"
     CDS             complement(6685..7296)
                     /locus_tag="PK754_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09857.1"
                     /translation="MTIVEIMLYATAFVLVLMALGGGLLIAGMSRSLGKLKERVRALE
                     HIQEERAAKKGAGTDDTTKIKSFETQIKALKAELESLRSMVVAPTPAEPAASDKEVFY
                     VTASTTDGFIRKKDLKVSPGFDSLFKVELNADKQTGTVTIWTGSENMTQAITFHDRYL
                     QPLCEYTYNPQRSTRNVTVKKPGMIKLESDRYVIVEKMVIEFE"
BASE COUNT         2156 a         1747 c         1474 g         2192 t
ORIGIN      
        1 gggcatggca tacccgtatg cttcgcgcac ctgctgatcc gctttgcgca tatcccagcg
       61 cgcaacggca agtaaagcgt tgtttttttc ggcgatacga atcgcttcct ccagcgtcaa
      121 cagcgaatcc tgcgccgcca gccatcctcc ggtacatata cataataaga accaacatcg
      181 tcgcatgaaa caatctcctt tacttaaacc gtacagtcta tttcctctat cggtccggca
      241 atcaactcca tctgtcttat cacaaagaat tgcaattcca tactttttca atcacgtgta
      301 ccgtgcacat acatcgccct gatctaccca ctaaaaccat catcgcggtt atcatgattt
      361 ttcgttgcac gtttttttgc tatttcactt tttacaaagt caccatattt taaaatctga
      421 ttccaaattt tcagatcgcg aagtaggatt ttgtttgcct tgcgttagat cagaaaccct
      481 gcgcgtcaca tagtcgcgaa gttcggaaac cgtgatcgat cgatcctgat ttgtatcggc
      541 gtttttagat tttaaacctt caatcaaact atatgtaaat acgccgtttt tccaagcctc
      601 tgactcaaac gcatattctt tacccgcggc ggatgagatc actatcgtac ccgtgccacg
      661 tcgtagatcg gcaaataatg ccttcatcaa ttcaaaacta ttgatcaaac cgaccgaatt
      721 tttattttga agtgttttga acccgcggga tgatacacct ttactttcct ggtgcacggc
      781 gacatttgtc aatacggttt cttctttatc aacttcgccg ctatggcacg catcaatcag
      841 cataagtttt ttgcgcgctg caatcccttc tgttagcgat tccagttcat catacgataa
      901 accattgaaa gatggacgat taaaatcaac atcatgcgta gcgatgtaat aattgagttg
      961 atcatccaat aaaccgtgcg acgcgacgaa aagcaagact tcatcgttta cagtggattt
     1021 cattaaaagg tttttagttt tcacaatatt attacgcgtg gcttgcgaat tggtcaatgt
     1081 ttgaataacc acatcggcat aggcatcttt attaaccgaa aacagtgacg ccatatcttc
     1141 tgcatccttg gcggcatacg tcagattcat attggagtca ataaactgcg aaacgccgat
     1201 ggccacaacg tacaaaacag gtttggtcgg aacgtgtgga cgatagtaaa cttctaagga
     1261 tgcgcttacg gactcaacgc ctcttgtgtt taaaaccgat gcacgaatga tattacggcc
     1321 ttccgataac gttaccttaa tcgtctgatg actgttgagc atgttttcgg ctttaatgct
     1381 ttttccccgc acaccgtaaa gaggaactcc gttaacttct acaaacaaac gatccaaagc
     1441 atataattta tctttgagat gaacactaaa agtataggta tcgttttgtg tttcaataag
     1501 atcggttttc aaatccggaa tggtaatttc cggtacatta aagcttcgtt ccgactcaaa
     1561 attcaccgga tcaaaaccca ttttttgcaa tcgcttcaaa taggcatttt tgtaagttcg
     1621 aataagttcc ggcggggcct tgcctaaacg ttccagaaca atatccgggc gattgtactg
     1681 taaatcaaat tgatcaaaat tataaatttt tttcccgcta ctataataaa tatggtcggt
     1741 aatatttttc gtggccatat aataaccgtt atcaacgacc gtcacccaac cattcccttg
     1801 atcaaacccg atgaacttgg ctatttcttt tttgctctgc cgattccaca aacgtacgga
     1861 tccgtcatta cccgctgata aaaaaaattg ttccttattg atgaaattca actgattgac
     1921 ttggccgaaa tgccctctat atgcaccgat tttttttccg ctatctaagt cccactccat
     1981 aattccttcc aacgggtcac tttcattgta acacgcgcct aaaaccgatt tattatccga
     2041 tggcaagagc gcagatcgaa ccgttccatt catcacaaaa gattgtcgga accgaccggt
     2101 attaacgtcc atgaggtaat ttttaatata gaatccgaag aaagccgttt tctcatcatc
     2161 ggataacgct atactgtagt taatagaacc gggaccagcg tacaattttt ttattttttt
     2221 agtctgccaa ttccaccata tagggcactt attgtatccg cctatcatta gcgacccatc
     2281 tttgaagaac tgaatttgat gtaccgaatg cgtgtccata ttcgcaccga agtatttatt
     2341 cggatttgta aggtcaaata tttttagatt aggtctatca tatcccgtca atcctccgac
     2401 ggccagcgtt tgcccatcat gagaaaatcg tagtgcattt ataaaatcag ttttgtcttc
     2461 atcggacgtt ctgaattgat tgatcacgga gttagatacg atattcagta tatgaatcgt
     2521 atctccgatc gccgcagcaa gaagacggcg attaggatta aatgcataat gacgaaccgg
     2581 tgagatcgca tagttatact gcggtttgcc gtacgtcaac gaccacactg aaacaaacga
     2641 tttatatccc acaataattc tttcttcatc cggatctgta aaaatagcca ttgccggttc
     2701 tgttttacgt ccaagcatcg ccgtggacga atccccgtcc atatgcagca aaaccatact
     2761 ggtattccat tctgtcccgc aaagtaagat ctttttgttt gcactatagc ctaataccgt
     2821 tggcgctcca agttttgaag tcaaatcttt atcggtcagc ggagttgtaa ttccgttcac
     2881 tatatccgtt ttaaagataa gaccgtcata ggatgccgac atgagttgat ttcgttcggc
     2941 gaaacacaag gccgtaatat gcttcttgtg aatatttatt tcagacttcg ttttcttttc
     3001 gttcaggtca aacacgaata attttttatc gtatgtacct gccgccgcca acaaaccgtc
     3061 gttcgaaatg cagatatccg ttatttggcc caagacttga ttattcccat cgcgcagaac
     3121 cttaatttcg gattgcgtac tgaaatccca cattcgtact gtaccttttc gatctccggt
     3181 caaaacagtt tgaccgtgca cggcgatacg cgtgattttt tcttcggatc ggcaaaatat
     3241 tccgatttcc gcaccggaca tggcatcata cattttgacg gcatattctg cgattactaa
     3301 gaccgttcta ttatccgatc caaattgaag cccgcgaata gaaaggttac ctcccatttg
     3361 actcctaccg agcataataa catgcacaat gctgtcactg tccagatccc atatagacac
     3421 attcccgtag ttatctcccc atgcggctaa gcgttgatcg gatgaaatat gtacggacat
     3481 cggaaaattt aatacgccat cgctgttaaa attggtaaac cctttacgcg aataaatttc
     3541 gcgcccccga tgtatatccc atagtttgag cgtattgtct ttactgccgg tgacagcata
     3601 acgcccgtct tccgtcatcc aaaagcatgt cacgcccaac gtatgatgcg atggtatcac
     3661 aatacgcaca ttgtgttgcg cattggcaaa gctagtaaac aacaaaaaca acattacctt
     3721 cagaagtaaa taccgcgtgt gatgcatagc gctacctaag aatttataat tacaaagtat
     3781 cgggtttggc gaatcaactg atgagaggac tttcatagag taacctgaat caaaaaaaaa
     3841 taggcgcatg aaaatgtacg ttttactctc tgtaaatcaa tgacaaatgt aagaaaataa
     3901 catcaaagaa aactcaaaat acagaaaaac gattctaaga aaaatgaata cagctataag
     3961 tgaattatag tccacttatg cagcgttata ttgcttcgga taacattgca ttatcttttt
     4021 cccgcaaaca aaaaacctcc gtttttgcgg aggttttata gcatcacatt gtacaaaaga
     4081 ctactgtcca ccaccttgca tgatgatggt cgcacgggct ttggcggctg tatcaaatgc
     4141 gccaaaatag gcacgcagtt cgtattcgcc ttcacccaag gttacactgt ttatttcgac
     4201 aacgccgtct ttttctatcg catacacgta ttcaccgcgt tcggtatctt ttttcccttt
     4261 aggtacgacc gtaatccaaa cggacggact gccgggaaaa ttaccatact ccaccgggat
     4321 aatctggcca atgagataat gtgaattttc agtacgtaca tacggcccgg tcggtttttt
     4381 tacttcttct tttgccgcta aaacaaaaat gctgttttta agtacgactt ccgctccgcc
     4441 ccaaccgata ttgcaggcat tcgcccaaag tttgccacag tgttcgccgt ccttatacgc
     4501 atgtttggcg atatacaatg gctctttttt agccgcatca ttatagccgc ccaaaatagc
     4561 attggcaggc gtcgtacctt tgtacgcgac ccaggttacc gcattggccg gtgcggttag
     4621 gatttcaaaa tctttatcat aggaatattc tttaccgccc catcctatat tacaataccc
     4681 gccgcctata acttttccgg gatgcatccc gccctcaaat gctacacgcc cgacatacat
     4741 cggttgaccg ttttcgtaac cgccgacgac cgcattttcc ggaattacac cggtaaaagg
     4801 aacccatttg gtcaaagatt cggtcgatgg tgtcataccg gttacggtct ttttaacagc
     4861 attcgaacct cctttggctc cctgcgcata gccattcaca gcaacgtgca gccacatgaa
     4921 taacgcggcg caaacaagca ataatgaaaa tctgttgaat cgcatgtacg aatactcctt
     4981 agtaagtaaa aaaatggtag aacagtaaaa cggatgatct cgtttatggt cgaaagcgga
     5041 atgaatggta gaaccgatta cacgaatgga agatagcgta gccgttagtc aaagtaaaca
     5101 atcaatatga gatatgcatt ccggtgccgt atcacatcag ggcgatgctt attttttcca
     5161 atcactagca ctctctctca atccggtatg atctgcctcc cctctgttgc gcacgcaacg
     5221 tgctgacttt tgtgcaacaa atcaaaagta tcgccgtaaa cctctattcg actaaccgcc
     5281 tttcaaagcc cattaggtac tcatacggca tcatgatcta gcgatgaaaa aaaataccga
     5341 tttttctcga ctgtaacttt ttcacgccgc ttgacaagac ggattgctac gtttatcgaa
     5401 tttccctcgt aaacacatag gttcgtttgt ttattaccac caatgaaagg atgatgagat
     5461 gaagttcagc aagctactac cgttagttat ggcgtttata acgatttcgt tttctatttc
     5521 gtacggtcaa acgtggcagc ctgccggagg cggaagtaaa aaagccagcg tatcggaatt
     5581 gataggactt accaacgtca cggtcgaata ctaccgtccg gcggtcaacg gacgagaggg
     5641 taaaatttgg ggaaacatag tccattatgg ttttgcggat ctgggatttg gcacgacgaa
     5701 agctgcaccg tggagagccg gggccaacga caataccact atcgaattta cgacacccgt
     5761 ttttattgag ggcaaaccat tgccggccgg aaagtacggt ttttttattg ccatgagtga
     5821 aacaaaagct acattaattt tttcgaaata taataccgca tggggaagtt tttattatga
     5881 cccgaaagac gatgcgctac gcgtggatgt acccgtcata aaaaccgccg aaagcgtaga
     5941 gcgtttgaaa tacgaattca gcgatcaaac ggatgacagt gctgttcttt cgctattgtg
     6001 ggaaaaagta aaaatccctt ttaaagtatc cattgatgcc aaaaaagaac aaatcgaagt
     6061 ttttcgtaaa gcgttcaatt cggcaaaatt ttatgtgtac tggcaaaata tgcatgccgc
     6121 ggccaattat tgccttgtca ataacatcaa tcttgaggaa ggtatgggat ggtctgagcg
     6181 atctattaat gattttttcg gcgaggccaa cttttcaacc ttatccacgt acgcgggatt
     6241 tcttgaaaaa tttaatcgta agaaggaagc ggattcggta atgcaaaaag catttccctt
     6301 gggcaccgca cttcaaatat attcctacgc acgcagtctc acgaaaatga aagaaaatca
     6361 aaaagcttat gaggttttca aaatcggtta cgataagtat ccgaatgatc cgaatattat
     6421 cttaggaatg gccagaggca atgcagcgat cggcaatatc aatgaagcgc ttaatttttg
     6481 tgaaaaagcg ctgactttgt ttgccgatcc ttcatccacc ggtattatca aaaaaatgat
     6541 ggaagacctc aaggccggca aagatatcaa taaataatca tcatagaagt tcaatacaaa
     6601 gaaaccgggt acagagaata gcgaggttca aaaatcgggt atgtgaaaac tgaattttaa
     6661 tgaggcgttt attttttaca ataatcactc aaattcaata accatttttt ctacgatcac
     6721 ataacgatcg gattctaatt tgatcatacc cggttttttt accgttacgt tgcgcgtaga
     6781 gcgttgcgga ttgtaggtgt attcgcacaa cggctgaagg tagcgatcat gaaacgtgat
     6841 cgcctgcgtc atgttttcac tgccggtcca gatagttacc gtgccggttt gtttgtcggc
     6901 attcagctcc actttgaata acgaatcaaa acccggcgac actttgaggt cttttttgcg
     6961 gataaatccg tcggtggttg atgccgtcac ataaaaaact tccttgtctg aagcggccgg
     7021 ttcggccggc gtcggtgcga cgaccataga acgcaaagat tcgagttcgg ctttgagcgc
     7081 tttaatctgc gtttcaaaag attttatttt cgtcgtgtca tctgtacccg cgcctttttt
     7141 agccgctcgc tcttcctgaa tatgttccaa cgcacgcaca cgctctttga gtttaccaag
     7201 cgaacgggac atcccagcta tcaataatcc gcctcctaag gccatcagca cgagcacaaa
     7261 agctgtcgcg tacaacatga tttcaacgat cgtcatgatt tattccttta tagttcgcac
     7321 atcggtcgta cattctatga ttttacaatg gataatccgg aactgatgga aaccttaaaa
     7381 caagcttagc ctacttattc ttttacgaac gtcgcgcgca tttttttaaa gcactttgga
     7441 caaattgtaa aacgcatgca cgagcggata aaaaaacaaa gtttctttta cagggtgatt
     7501 ctccatagtg ccgtctaata atacctgatg acttattccg tcgctgataa ggtgtataat
     7561 atctttttg
//
LOCUS       DAOQNQ010000151         7535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3681_length_7535_cov_6.118449, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000151 DAOQNQ010000000
VERSION     DAOQNQ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7535
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3681_length_7535_cov_6.118449"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PK754_09890"
     CDS             <1..724
                     /locus_tag="PK754_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HNB09858.1"
                     /translation="FIFKIQANMDPKHRDRIAFMRVCSGKFSRDMMVTHTRTGKKVRL
                     SNSHKLFGQQRETVDDAYAGDIVGLVGHSEFGIGDTLTEDQTIVYKEIPRFPPECFSF
                     LHNPNPSKFKRFREGLEQLLQEGVVQAFYLKNSYEKVPILAAVGPLQFEVVQFRLESE
                     YGAECRLESTNWKVVRWVSPEADWEKISKIYLPTGAAYAEDMAHETVILFPDEWTCNY
                     FIEKNPEVQLSSFSYKDATVLD"
     gene            complement(721..1368)
                     /locus_tag="PK754_09895"
     CDS             complement(721..1368)
                     /locus_tag="PK754_09895"
                     /inference="COORDINATES: protein motif:HMM:NF039470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6483 family protein"
                     /protein_id="HNB09859.1"
                     /translation="MIHRDYIMRLVDLCAKALARVMNMRQPQEQQQALEDIRSTLAQA
                     VSVSPDLLDSMTEMDLITLLRKSEWTAELFDSVGELLLQQGRILKSENKEMEAARVFR
                     KAWLCFYEASLNVGDFQSRSEKMDFCITHDILYAGNPGYYQTVLEWLTRAGRYEKGED
                     WLFDMAEAGIAADSDAEKFFGWLKLMPDDVLKKSGMPRDEVISSQETYDRKFKRV"
     gene            complement(1436..2086)
                     /locus_tag="PK754_09900"
     CDS             complement(1436..2086)
                     /locus_tag="PK754_09900"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNB09860.1"
                     /translation="MRLLLLGAPGVGKGTQSKLLVEEFKIPQISTGDMLREAIRNNTP
                     LGLKAKSYMDQGQLVPDEIIIGLVEERLRLDDAHYGFILDGYPRTIPQAEALDALLSR
                     MSIQIDAVIDINVDSEELVNRLSHRIICRSCGAVYNKVTNPPKVEGVCDKDGGEIYQR
                     SDDKKEAIENRLKVYEKETAPLREYYRKRGKLHAIDGQLPMGVVYKKILAILEVEK"
     gene            complement(2101..2817)
                     /locus_tag="PK754_09905"
     CDS             complement(2101..2817)
                     /locus_tag="PK754_09905"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09861.1"
                     /translation="MDTAIIDWRTPAIKRRLIEDDPLDWPTIFGNHNPIDLEIGIGNG
                     SFLVPFCAHNKLRNMVGIEIDGFYLKKADRKLNQTGLTNGRLLIGDAKLFTWRLFGKE
                     TIADVYINYPDPWFKKRRLKRRVINTLMLQMLATRMTGVLTIATDDADYRDWVFEAIR
                     DSGCWESLIPEGFSSQLEGYIQTKYERKWKAMGKPCYYMRYRKTTNPVFSTDHYITEQ
                     NLQFPLRKYAEELQSDIVEL"
     gene            complement(2825..3607)
                     /locus_tag="PK754_09910"
     CDS             complement(2825..3607)
                     /locus_tag="PK754_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005829231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNB09862.1"
                     /translation="MSTFQYLTLEIAEKIAIVGLNNPPGNSLKMPLLQELDRMMDELH
                     ANPEAKVIVITGAGKTFASGADIEEISKLTSQQQAKEMSAFGQKVFLKIENGPKPVIA
                     AINGFCLGGGLELAMSCHMRVASDRSFMGLPEITLGIIPGFGGTQRLPRIVGLSKASQ
                     MILSGDQVRSEEALAIGLVNMVVSKDTLLDDVKKLAAKMTNKSAVAIKQALKSMKQGA
                     SLEMAASMEVESSCFAELYNSADMKEGVSAFLEKRKPNFQDR"
     gene            complement(3668..4225)
                     /locus_tag="PK754_09915"
     CDS             complement(3668..4225)
                     /locus_tag="PK754_09915"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNB09863.1"
                     /translation="MRVRKFFLLLWIAQLTSLTAQSENTLQPGSTAPNFFLKYLDQSE
                     FYSKDYYGEPRSLPKAKKERNAVIISFFASWCGPCRKEIPELEKLQSKYADVRIFLIN
                     VAESQDKVLSFLAENPVSLPVLMDIYGKTAEKFDVKDKGLSLAVLPTLVMIRKDGTVH
                     FYKKGYTAGDEKKIEEQILTLLAQP"
     gene            complement(4339..4782)
                     /gene="rplI"
                     /locus_tag="PK754_09920"
     CDS             complement(4339..4782)
                     /gene="rplI"
                     /locus_tag="PK754_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNB09864.1"
                     /translation="MKVILKQNVDTLGQAGEIVDVKPGYARNFLVPKGFAMVATKANE
                     AAFENEKRMIAARLAREKALAQEMVQKLEAISVTIPVATGEEDKLFGSVTSQDIAEYL
                     AQQGHDIDKRKILLDEPIKALGLYNVDVKLFTDVIGKIKVWVVKK"
     gene            complement(4818..5084)
                     /gene="rpsR"
                     /locus_tag="PK754_09925"
     CDS             complement(4818..5084)
                     /gene="rpsR"
                     /locus_tag="PK754_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNB09865.1"
                     /translation="MAEKKFEKKRREPRGGMNKPKHCPFCEEKALYVDYRDTKRIMKF
                     TSEQGKILARRTSGVCATHQRQLVSAVKYARHLAMIPFVSDMAR"
     gene            complement(5118..5513)
                     /gene="rpsF"
                     /locus_tag="PK754_09930"
     CDS             complement(5118..5513)
                     /gene="rpsF"
                     /locus_tag="PK754_09930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNB09866.1"
                     /translation="MSKKTRKGSAGQSRFYETVVVFDAQTGDDVIKGIVDKIKEVLTS
                     HSAVNVAVDEWGKRKLAYVINKKTYGHYVCVEFEAPGSALQALNDYYHITEQIMRHMT
                     MLVDSRLRAERKREKTAMPKEAVADEAAI"
     gene            complement(5654..>7535)
                     /locus_tag="PK754_09935"
     CDS             complement(5654..>7535)
                     /locus_tag="PK754_09935"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNB09867.1"
                     /translation="VSGSMEEHSSWLVGISFAVGAFCSALAGFFGMKVATKANVRTTN
                     AARTGLQNALQIAFSGGSVMGMCVVGLGVIGLTVLYFVYSNMFTDTAKVLNVISGYSL
                     GASSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDVAG
                     MGADLFESYVGSIVGTMILGAAMIVGTNAGMVPVLLPILLAGVGIIASIVGTFFVRTK
                     EGGDPQVALNTGTFVAAGAMIVASYFAIHKLWPSVDLYDTMDTAKLVTANDVFLATVF
                     GLIVGVLVGIVTELYTGGGRSHVNRIVEQSVTGPATNIISGLAVGMESTAIPVVLIAV
                     GIIGSYYFAGLYGIAVAALGMLSTLGIQLAVDAYGPIADNSGGIAEMSHLPEEVRGRT
                     DKLDSVGNTTAAIGKGFAIASAALTALALFTAYMSQVGLKTLDVANPKIMAGLFVGGM
                     LPFLFSSMAMSAVGKAAMDMVKEVRRQFKEIPGLLEGKPEAKPDYKRCVEISTQAAIR
                     EMVVPGLMAIAVPVAIGFIPGLGKEALGGLLAGCTVTGVCMAIFMANSGGAWDNAKKS
                     FEGGYTLKDGTVVKKGSDAHKAAVVGDTVGDPFKDTAGPSLNILIKLMSVVALVIAPL
                     LK"
BASE COUNT         2061 a         1867 c         1559 g         2048 t
ORIGIN      
        1 ttttattttt aaaatacaag ccaatatgga tcctaaacat cgcgatcgta ttgcctttat
       61 gcgcgtttgt tcgggtaagt tttctcgcga tatgatggtt acgcatacac gaaccggaaa
      121 aaaagtacgc ctgtccaatt cgcataaatt atttggtcaa cagcgggaaa ccgtagatga
      181 cgcgtatgcc ggtgatattg tagggttggt cggtcactcc gaatttggca tcggagatac
      241 gctaacagaa gatcaaacga tcgtgtacaa ggaaataccc cgatttcccc cggaatgctt
      301 ttcgttctta cacaatccca acccatcaaa atttaaacgt tttcgagaag ggctggaaca
      361 gctattacag gaaggcgtgg tacaagcttt ttatttaaaa aattcttacg aaaaagtacc
      421 gattttggcc gccgtcggac ctttacaatt tgaagtcgta caatttcgcc tggaaagtga
      481 atacggagca gaatgtcgac tggaaagtac aaattggaaa gtggtaagat gggtttctcc
      541 cgaagccgat tgggaaaaaa tttcaaaaat ctaccttccg accggtgcag catacgccga
      601 agatatggcc cacgaaacgg ttattctctt tccggatgag tggacgtgta attattttat
      661 cgaaaaaaat ccggaagtgc agttatcatc cttttcttat aaagatgcca ccgtactgga
      721 ttaaacgcgt ttaaattttc ggtcatacgt ttcttgcgat gaaatcacct catcgcgcgg
      781 catacccgat tttttgagta cgtcatccgg cataagtttc agccacccaa aaaatttttc
      841 ggcatccgaa tctgcggcga tgccggcttc cgccatatca aataaccaat cctcgccttt
      901 ttcataacgt ccggctcgag ttaaccattc caatacagtc tgataatacc ccggatttcc
      961 tgcgtacagt atatcatggg ttatacaaaa atccattttt tcagatcgtg attggaaatc
     1021 acctacgttg agactggcct cataaaaaca cagccaagcc ttacgaaaaa cacgcgccgc
     1081 ttccatttct ttgttttcac ttttcagaat gcgcccttgt tgtaataaca gttcaccgac
     1141 gctatcaaaa agttcggctg tccactctga tttcctcagc aaagtgatca aatccatttc
     1201 cgtcatacta tccagaagat ccggtgaaac ggaaacagcc tgtgctaaag tagaacgaat
     1261 gtcttccagc gcttgttgtt gttcctgcgg ttgccgcata ttcataacgc gtgccaaagc
     1321 tttggcgcag agatcaacta agcgcatgat ataatcgcga tgtatcatag tttgtctaac
     1381 actttaaaca taaaaaaagc cgcgtgatgc ggctttatta ctttgggatc aaatttcatt
     1441 tttcaacttc taaaatggcg agtatttttt tatacacgac acccatcggt aattgtccgt
     1501 ctatagcatg gagtttgccg cgtttacggt aatattcacg aagcggagcc gtctcttttt
     1561 cataaacctt gagccggttc tctatggctt cctttttatc atcggatcgc tgatatatct
     1621 ctccaccgtc tttatcgcat acaccttcga ctttgggcgg attggtcact ttattgtaaa
     1681 cggctccgca gctacggcat ataatacggt gcgaaaggcg gttcacgagt tcttcggaat
     1741 ccacattgat gtcaatcacc gcgtcaatct ggatgctcat gcgcgataac aaggcgtcta
     1801 acgcctctgc ctgcgggata gtccttggat atccgtcaag aatgaaaccg taatgcgcat
     1861 catcaaggcg caagcgttcc tcgacaaggc cgatgatgat ttcatcaggt actaactggc
     1921 cttgatccat gtaggatttg gctttaagac cgagcggcgt attattccga atggcttcgc
     1981 gcaacatatc accggtggaa atttgcggaa ttttaaattc ctccaccaat aatttggatt
     2041 gcgtgccttt accgacaccg ggggcgccca gcaacaataa acgcatgcga atgattcctc
     2101 ctataattca acaatatcag attgtagttc ttcggcatat ttccgcagtg gaaactgcaa
     2161 attctgttcg gtaatataat gatccgtcga aaaaaccggg ttggttgttt tccggtaacg
     2221 catataataa cagggtttac ccatcgcctt ccatttgcgt tcgtattttg tttgaatgta
     2281 gccttctaac tgtgacgaaa aaccttcggg tatcagagat tcccaacaac cggaatcgcg
     2341 aatcgcttca aacacccaat ctctatagtc tgcatcatcc gtcgctatcg tcagcactcc
     2401 ggtcatgcgc gtcgccaaca tctgaagcat caacgtatta atcacgcggc gcttgaggcg
     2461 tcgttttttg aaccaaggat ccgggtaatt gatatacaca tcagctatgg tctctttgcc
     2521 aaataagcgc catgtgaaca gcttagcatc tccgatcaat agtcggccat tggtcaaacc
     2581 ggtttgattt agttttcgat cggctttttt gaggtaaaaa ccgtctattt caattccgac
     2641 catattgcgc aatttgttat gtgcacaaaa aggaaccagg aagcttccgt taccaatacc
     2701 gatttctaga tcaatcggat tatgattacc aaagatcgtt ggccaatcaa gcggatcatc
     2761 ttcgataaga cgtcttttga ttgccggcgt tcgccaatca attatcgctg tatccatagt
     2821 tcgatcagcg atcctgaaaa ttgggcttgc gcttttcgag aaatgcagat accccttctt
     2881 tcatatcggc tgagttatat aactcagcaa aacaggatga ctcgacttcc atcgaagccg
     2941 ccatttcaag actggcgccc tgtttcattg acttcaaagc ttgtttgatt gctaccgcgc
     3001 ttttattggt catcttggcg gccagttttt tgacatcatc aagcagcgta tccttagaaa
     3061 caaccatgtt caccaaaccg atagccaatg cttcttccga acgcacctga tcaccggaca
     3121 aaatcatttg tgaagctttg cttaaaccga caatgcgggg caggcgctgc gtaccaccga
     3181 atccgggtat aatacccagt gtgatttctg gaagccccat aaaagaacga tccgaagcaa
     3241 cacgcatgtg acaactcatg gctaattcca atccaccgcc gaggcaaaac ccgttgatcg
     3301 cagcgatcac tggtttggga ccgttttcta tttttaaaaa cactttttga ccgaacgccg
     3361 acatttcttt tgcttgctgc tgggatgtca attttgaaat ttcttcgata tccgcaccgc
     3421 ttgcaaacgt ttttcccgca ccggtaataa cgatgacctt ggcctccgga ttggcatgca
     3481 attcatccat catacgatca agttcttgca acaaaggcat cttaaggctg ttgcccggag
     3541 gattattcaa tcccacaata gcaattttct ctgcgatctc aagcgtcaga tactgaaatg
     3601 ttgacattgg cgcttccttt tttggttcag aatatattat tcagagcgga caattcaaac
     3661 tgtaaaatca aggttgggca agtagagtca gaatttgctc ttctatcttt ttttcatctc
     3721 ctgccgtgta cccttttttg taaaaatgca cggtaccatc cttacggatc attaccagcg
     3781 ttggcagaac agccagggac aaacctttat ctttgacatc aaatttttcg gctgtcttac
     3841 cgtaaatatc catcagaacc ggaagcgaca cagggttttc tgcaagaaaa ctcagcactt
     3901 tatcttgaga ctcagcaacg ttaatcaaaa atatgcgtac atcggcgtat ttggactgta
     3961 acttttcgag ttccggtatt tctttacggc atggtccaca ccaactcgca aagaaactaa
     4021 taatcactgc atttcgttct ttctttgcct taggtagcga tctcggttcg ccataataat
     4081 ctttggaata aaattcgctt tgatccagat atttcaaaaa aaagttcggc gccgttgagc
     4141 cgggttgcaa agtgttctca ctttgcgcgg tcaatgacgt caattgggct atccataata
     4201 acaaaaaaaa tttacgcaca cgcattatcg gctattctgt taaatccgaa taatgtaagc
     4261 ggttcgaata acaaataaaa cagaaaaggt aacctgtaga gcaggttacc ttttcacaaa
     4321 caagtacctg gcgttgcgtt atttttttac aacccaaact ttaatttttc cgatcacatc
     4381 cgtaaacagc ttaacatcca cattgtacaa accgagcgct ttgatcggtt cgtccaacag
     4441 aatcttacgc ttgtcaatat catgcccttg ctgtgcaaga tattcggcga tatcctgaga
     4501 ggttacagaa ccgaagagtt tgtcttcttc gcccgtagcc acgggaatcg taacggaaat
     4561 agcttcaagt ttttgaacca tttcttgtgc caacgctttt tcgcgcgcga ggcgtgctgc
     4621 gatcatgcgt ttttcatttt caaaagctgc ttcgttggct ttggtcgcca ccatagcaaa
     4681 acctttgggc acaagaaaat tgcgggcata acccggtttt acatcgacga tttcgccggc
     4741 ttgtccgagc gtatcgacgt tttgttttaa tatcactttc ataaaaaatc tccagagttt
     4801 cttcgttgtt accgatttta acgagccata tcactgacga aaggaatcat cgccaggtga
     4861 cgtgcatatt tgactgcgct gaccaactga cgctggtgtg tagcgcatac gccggaagtg
     4921 cggcgtgcca aaattttgcc ttgttcagaa gtgaacttca taattcgttt ggtatcacgg
     4981 taatcaacat acagcgcttt ttcttcgcag aacgggcaat gtttgggttt gttcattccg
     5041 ccgcgcggtt ctctgcgttt cttttcaaat tttttctctg ccatggtcgt atcgttcctt
     5101 acgttttcgt taaatgatta aatcgcagct tcatcggcaa cggcctcttt aggcatcgcc
     5161 gttttttcac gtttacgttc ggcacgtaaa cggctgtcta ccaacatggt catgtgacgc
     5221 atgatctgtt cggtgatgtg ataataatca ttcaacgctt ggagagccga tcccggcgct
     5281 tcaaactcga cgcacacata gtggccgtaa gtttttttgt tgatcacata cgccagtttg
     5341 cgcttgcccc attcatctac ggcaacattg acggcactgt gacttgtgag cacttcttta
     5401 attttgtcaa cgatgccttt gatcacatcg tcaccggtct gcgcgtcaaa tacaacaacg
     5461 gtttcataaa accgtgattg tcctgccgag cctttgcggg tttttttgga catggagtct
     5521 cctttggact atgttcggcg tcagcgtttg ttaacacaac ttacgttagc aattctgacg
     5581 caggattatg atgattctaa tttattaaaa gggattcaat acatacgaag tacaaatccc
     5641 tttgataatt cacttactta agcaacggtg caatcaccag cgctacaacc gacatcagtt
     5701 tgatgaggat gttaagcgaa ggacctgccg tatctttgaa cggatcacct acggtatcac
     5761 ccacgacggc cgccttgtgc gcatcggagc cttttttaac aaccgtaccg tctttgagcg
     5821 tgtagccgcc ttcaaacgat tttttagcat tgtcccaagc gccgccggag ttagccataa
     5881 atatagccat acatacgccg gtcacggtac aacctgcgag caaaccgccc aaagcttctt
     5941 tacctaaacc gggtataaaa ccgatcgcaa ccggcacagc aatggccata agaccgggca
     6001 cgaccatctc gcggatagcg gcttgagtgg aaatttccac acaacgtttg taatccggtt
     6061 tggcttcggg tttaccttcg agaagaccgg gaatttcttt gaattgacga cgcacttctt
     6121 tgaccatgtc cattgcagct ttaccgaccg cgctcatcgc catggatgag aaaaggaacg
     6181 gaagcatacc gccgacaaat aaaccggcca taattttcgg attggccaca tccagtgttt
     6241 tcaaaccaac ttgactcata taggccgtaa ataaagccaa cgccgtcaaa gccgccgacg
     6301 caatggcaaa acctttacca atcgcagctg ttgtattacc gacagaatct aatttatctg
     6361 tacgaccacg aacctcttcc ggaagatgcg acatttcagc gataccgccc gagttgtcag
     6421 cgataggacc gtacgcatct acagcgagct ggatacctaa tgtagaaagc ataccaagag
     6481 cggcgacagc tataccatac aaaccggcaa aataatatga accaataata ccaaccgcaa
     6541 tcaagacaac gggaatggcc gtagattcca taccgacagc aagtcccgag ataatgtttg
     6601 tcgctggacc ggtcactgat tgttcgacga tacgattgac atgcgaacgt ccgccgcccg
     6661 tgtacagttc ggttacaata ccgaccaaaa caccgacgat caaaccgaat accgtagcca
     6721 agaatacatc attagccgta actaacttgg ccgtatccat cgtatcgtat aaatctaccg
     6781 atggccaaag tttatggatg gcaaaatacg aagcgacgat catagcgccg gctgcaacaa
     6841 aagtacccgt attcaacgct acttgcggat caccaccttc tttggtgcga acaaaaaatg
     6901 taccgacgat ggaggcaata atacctacgc ccgctaaaag aatcggtaac aataccggca
     6961 ccataccggc attggtgcca acgatcatcg ccgcacccaa aatcatcgta ccgacgatag
     7021 agcccacata cgactcaaaa agatcagcgc ccataccggc cacgtcaccg acattatcac
     7081 cgacattgtc agcgataacg gcaggattac gcggatcgtc ttcgggaata cccgcttcta
     7141 ctttacctac aaggtcagcg cctacgtctg cagctttggt ataaataccg ccgccgacac
     7201 gcgcaaaaag agcaatcgaa gacgcaccga gtgaataacc tgaaataaca ttcaaaacct
     7261 tagccgtatc cgtaaacatg tttgaatata cgaagtacaa taccgtcaga ccgataacac
     7321 cgaggccgac gacgcacata cccatcaccg atccaccgct gaatgcgatt tgaagcgcat
     7381 tctgaagacc ggtacgcgcc gcattggtcg tacgaacatt agctttggta gccaccttca
     7441 taccgaaaaa tccggctaat gcggaacaaa atgcaccgac agcgaaggaa ataccgacaa
     7501 gccagctaga gtgctcttcc atggatccgg aaacc
//
LOCUS       DAOQNQ010000152         7534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3683_length_7534_cov_5.716005, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000152 DAOQNQ010000000
VERSION     DAOQNQ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3683_length_7534_cov_5.716005"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3165)
                     /locus_tag="PK754_09940"
     CDS             complement(<1..3165)
                     /locus_tag="PK754_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09868.1"
                     /translation="MKQRITIIFGVLYFFHQISVSAQVGTWENVGGDISGPSANAILK
                     MGSDVYVGGSFWGAAGVSGADNIVRWDGLQWVALGEGLNGTVNDMVFVGNDLYVGGFF
                     EDAGGDPNADFLARWNGVSWSAVGTGVSGYVDALATDGTNLYVGGNFTNAGGIANTFC
                     IAKWNGSAWSALGSSGLNSTVRTIAMINSTTFYAGGVFSNIGGDPNKSRIVKWNGSDW
                     EAVGAGLNSDVTSIAISGADVYVGGLFTNVGDVVGANYIAHWNGSTWSALGSPSEAVS
                     SVAVVGTEIFVAGNSNISNDAANPSGVAKWNGSSWESFGNFNSTVSALLASGGDLYIG
                     SPLTGSSSPIVWNGSTFSFFGSGLAQSVNTIAVSGENIFIGGGFTNAFQNSNINYIAM
                     RNGAVWSSLGSGLNGSVKSIAVSGSDVYVGGQFTDAGGITTADHIARWNGSAWSAMGN
                     INPGVQILSMAITGSTVYAGGVFTDLSGVSGASGIAKWNGATWEALGDGLFGGEGEAY
                     ITSIVISGSDVFVGGYFQNAGGISDADNIARWDGSTWHAVGNGLNEGVSCMVMNDGLL
                     YVGGEFSDVVGGVSNNLNRLAVWNGSAWQPLGSGLGNEDYVYSLACIGSEVYAGGNFE
                     EIGSTAATNIAKWNGIAWESVASGLNNTVNQMAVSSNDLIVGGEFTKSGTEVANKLAV
                     YHAPTPANPLVVTNNSDDNVVGTLRYATNYANAHPGLDTIRFDASMIGQKILTEGITI
                     PDETLVNGDINGDRAPDIAIQLINTVSDYALQIGNQVTIHAINISGSKNGIRITGSQN
                     TISNSYFGTNLSGSDTTGGFANQFTAIYGESAASFITIEGNLLAGRKDINGVTGLLLN
                     GGRGHLIRGNVFGTDKNGNLDLGFKNSSVVIDAKAFSFGDGSFSGRNIVVNNGNGGTT
                     VYLGGDSVRVLGNFVGLWKDGVTAKSNGSGMSVVGNYAQIGDGSNSGRNVIGNCANAA
                     LVIQGNYGRVLGNYIGTDSTGIVARGNTQGVYVGSGSHSVYIGDGTADGRNIICSNNI
                     GIYSDPAGTDSVYVLGNWIGIGADG"
     gene            complement(3310..3951)
                     /locus_tag="PK754_09945"
     CDS             complement(3310..3951)
                     /locus_tag="PK754_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB09869.1"
                     /translation="MIKVAVVEDDKIIRQSLQIIIDGTDGFRCIGAYPNAESFIDDVQ
                     RLKPEVVLMDIHLKNSMSGIEATRILRKAYSRLVILIQTVYEDDNTIFDALCAGANGY
                     LLKKTSPARLIDSIREAVEGGAPMSPAIAKKVVAHFQQNNIVSQNSEDARLSDRELDV
                     LRLIADGRPYKQASELLSVSVPTIRFHMGNIYKKLHATSQSEAIAKAIRRGLI"
     gene            complement(4022..6913)
                     /locus_tag="PK754_09950"
     CDS             complement(4022..6913)
                     /locus_tag="PK754_09950"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB09870.1"
                     /translation="MPSKHCAWSILLILCFIKLCPYPAAILLAQTHIHRNISTDHGLP
                     QSQVTALMRDTKGYLWIGMLGGLARWDGNEFYSIRLEDGSSPGGIRSLHEHVNGRIFA
                     ATNGQGLLEIIGQRAILHDKRSGLPDMSVRTIASDTDGALWVGTDKGLVRFTDAILDS
                     ASARIFFTGIVISDVKAYKNKVYAGCFDNGLYEFLNDTFEVLFPSLSSDIPKIRSFIY
                     VEAENEWYVAVDGRGTAKITNNSLTWLAPLSDYRVQTMRWIKDGGVFFTTIGNGVYNL
                     RNGKWLHIGEDQGLANNSVWAIETLENGWIALGTWGGLDFYDGGRIRSYRRENGLQDP
                     VVLAIEEDPRQAIYFGTATGGVSMLYKNKWQHWNKKNGFPDLTIWSLLSTSRENFFVG
                     TNNFGIIDITPSGSKKLYGKAEGLSENRVYVMRERSDGSVFIGHREGLDVLDKGKIYS
                     YPKGHRWNRAFITGIHAVSDDEIYLATRYGVMHVNKGIVDTINRKKGLSDDHVWNVYV
                     DSAQYIWASTNNGGITIFSNSTIDTINTRNGMSDNTVYGVIQYKENIYYAATHKGINR
                     IEIKNGHFEINVITKSEGLPSDELNQGAFRKNSDGTMWFGTTKGAVHYDPRMEVRQPT
                     PPNVHITRVRAFDNELLAKSASDVISLPYNENYIKFDFVGLNLHAPERVVYQYRLSSI
                     DREWVTSEHHFCQYPNLSDGTYTFEVRASNEYGLWSEICSYRIIIEPPYWKTWWFIML
                     VTLAGGGLISLIVYSRIQAMLAVERLRNKIAADFHDAIGAGLTQVHYLTQTALMHSEK
                     TEQVRHNLTIIDAISKSLYEEMRDIIWLVNPRRDSLYELILRLKESSEDMAEQSGVEF
                     ITENIDSLSGLRLAMERRSHIYLIFKEATANALKYSRCKTLTLTAHVKGDRLSIVLSD
                     DGVGFEDAHPRAGGNGMHNMKTRALEANGQIEIHSEPNKGTRVTFTGKI"
     gene            complement(6948..>7534)
                     /locus_tag="PK754_09955"
     CDS             complement(6948..>7534)
                     /locus_tag="PK754_09955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB09871.1"
                     /translation="KKLHAEMMPSLEGSMVGGMRIKAIIDNLKRFSNLNESDWKLISL
                     EDNLDIICDLFIKQYDFVTIEKKYNGLPAVYCNTAEINQALVNILTNAVQAIQDRGLK
                     EGQIDLSTSTQTVEQNEYVVVSIRDNGIGMSSAVMDKIYDPFFTTRDVGSGRGLGLTE
                     AYGIVQRHHGKIEVWSEVGKGSEFTVYLPVRKRP"
BASE COUNT         2133 a         1947 c         1427 g         2027 t
ORIGIN      
        1 cccatccgca ccgataccta tccaattgcc cagtacatat accgaatctg ttccggccgg
       61 atcggaatag attccgatat tattggagca aataatatta cgtccgtccg cagtaccgtc
      121 accgatgtat accgagtggc ttccgctacc gacataaaca ccttgcgtat tccctctggc
      181 gacgattccg gtagaatcag ttcctatata atttccaaga actcgtccgt aatttccctg
      241 aataactaac gccgcattag cacaattacc gataacatta cgtcccgaat tactaccgtc
      301 gccaatctga gcataattac ctactactga catccccgat ccattggatt tagccgtaac
      361 gccgtccttc cacagaccta cgaaattacc caatacacga accgaatccc ctcctagata
      421 taccgtagtg ccgccattac cattgttgac tacaatattt cgaccgctaa aactgccatc
      481 accaaacgaa aatgccttag cgtctataac gacagacgaa tttttaaaac ctaaatctag
      541 gttaccattt ttatcagttc caaaaacgtt tcctctaatc aaatggccgc gaccaccatt
      601 cagtaaaagc cccgttactc cattgatatc ttttcgtcca gccaaaagat tgccttcaat
      661 cgtaataaag gaagccgcgc tttcaccgta aatagcagta aactgattgg caaatccgcc
      721 cgtcgtatca ctaccggaca aattggtccc aaaataagaa ttggaaatcg tattttgtga
      781 gcctgtgata cgaattccat ttttgcttcc cgagatatta attgcatgaa ttgtaacctg
      841 atttccgatt tgcagagcat agtctgaaac tgtattgata agttggattg cgatatcggg
      901 tgcacgatcg ccattgatat caccgttaac caatgtttca tccggtattg tgatgccttc
      961 agtcaaaatt ttttgcccga tcatagatgc atcaaaacga atggtgtcca aaccgggatg
     1021 ggcattggca tagttggtcg cgtagcgcaa tgtaccaaca acattgtcat cactgttatt
     1081 ggttaccacc aaaggattgg ccggtgtagg cgcatgataa acagctaatt tattggccac
     1141 ttccgtaccc gacttcgtaa actcgccgcc tacgatgaga tcatttgatg ataccgccat
     1201 ctgattaaca gtgttgttca atccacttgc taccgattcc catgcaatac cgttccattt
     1261 ggcgatattc gttgcggccg tgctacctat ttcttcaaag ttgccgccgg cgtaaacttc
     1321 gcttccaata catgctaagg agtaaacata atcctcgttt ccaagcccgg atccaagtgg
     1381 ttgccatgcg cttccattcc atacggctaa gcgattcaga ttattcgaaa cgccaccaac
     1441 cacatcgcta aattctccgc caacgtacaa taaaccgtca ttcataacca tacatgaaac
     1501 accctcattg agcccattgc ccacagcatg ccatgtacta ccgtcccatc gtgcaatatt
     1561 gtctgcgtcg gaaataccac cggcattctg aaaatatccg ccaacgaaaa catctgagcc
     1621 cgaaatcacg atgcttgtaa tgtaggcttc tccttcgccg ccaaataaac catcccccaa
     1681 cgcttcccaa gtagcgccgt tccatttagc aattccgcta gcaccggaca caccactcaa
     1741 atccgtaaag acgccgccgg cataaacagt cgaaccagtg atagccattg ataaaatttg
     1801 tacaccggga ttaatgttgc ccatcgccga ccacgctgaa ccattccatc tagcgatatg
     1861 atcagccgta gttataccac cagcatcagt aaattgtcca ccgacataaa catcgctgcc
     1921 cgataccgcg atggacttca ccgacccgtt gagaccactt cccaacgatg accacacagc
     1981 cccatttctc atcgcgatat agtttatatt tgaattctga aaagcattgg tgaatcctcc
     2041 gccgataaaa atattctctc ctgacacagc gatcgtattg accgattgag caagtcctga
     2101 tccaaaaaaa ctgaacgtgc taccattcca cacgatcggg gagcttgacc cggtgagtgg
     2161 cgatccgatg tagaggtcgc cgccggaggc taagagtgct gataccgtgc tgttaaaatt
     2221 gccgaaactt tcccagcttg atccattcca ttttgctacg cctgatgggt ttgctgcatc
     2281 gtttgaaata ttagaattgc ctgctacaaa aatctccgta ccgactacgg ccacactcga
     2341 taccgcttcc gaaggagaac ctaaagcgga ccaagtagaa ccattccagt gtgcaatata
     2401 gttagcccca acaacatcac cgacattcgt aaataaacct cccacatata catctgcccc
     2461 ggagattgca atggaggtga catccgaatt taatccggcg cccaccgctt cccaatccga
     2521 tccattccat ttcactattc gtgacttatt gggatcgcca ccgatattgc taaacacgcc
     2581 gcctgcataa aaagtagtac tgttgatcat ggcgatagtt ctgactgtgc tgttgaggcc
     2641 tgacgaaccc agcgccgacc acgcgctgcc attccattta gctatacaaa atgtatttgc
     2701 aatgccgccg gcatttgtaa aatttcctcc gacataaaga ttagttccat ccgtagccaa
     2761 cgcatctaca taaccgctaa cgccggttcc gactgcagac caagacacgc cattccatcg
     2821 cgctaaaaaa tccgcattgg gatcacctcc ggcgtcttca aaaaaaccgc cgacatacaa
     2881 atcgtttcca acaaaaacca tgtcgttgac cgttccgttc agaccttcgc ctaaggccac
     2941 ccattgcaaa ccgtcccatc ggacaatatt gtctgcaccg ctaacacccg cggctcccca
     3001 aaaacttccg ccaacataca catcggaacc cattttgagt atcgcattcg ctgaagggcc
     3061 tgaaatatcg ccgccaacat tttcccacgt accgacctgt gccgagacac tgatctgatg
     3121 aaagaaatat agaacgccga atataatcgt aatccgttgc ttcataaacg atcctcctga
     3181 tgggtattac cgttggttat gaatcaaaat aaaacagact gtttatacgg aatactgtca
     3241 gaatggataa atagcgaagt atgaatgaga agcaacctat cttatctgat aggtcgtcaa
     3301 aaggtaggat cagatcaaac cgcggcgaat ggctttggcg atggcttcac tttgcgaagt
     3361 ggcgtggagt tttttataaa tattacccat atgaaaacga atcgtaggta cgctgacgga
     3421 aagtaactcg gaagcttgtt tataaggtct gccgtcagcg ataaggcgaa gcacatccag
     3481 ctcacgatca gagaggcgag cgtcttcaga attttgcgat acaatattat tttgctgaaa
     3541 atgagcaact acttttttgg caatcgccgg actcatgggc gccccgcctt cgacggcttc
     3601 gcgaatacta tcaattaatc gtgccggaga agtttttttc agcagataac cattagctcc
     3661 ggcgcataac gcatcaaaaa tcgtattgtc gtcttcatac acggtctgta taagaatcac
     3721 caaacgacta tatgctttac gtaagatacg cgtcgcttca ataccactca tactgttttt
     3781 aagatgaatg tccatcaaca ccacctccgg ttttaaccgt tgcacatcat ctatgaaaga
     3841 ctcggcgttg gggtaagccc ctatacaacg gaacccgtcg gtgccatcaa taattatttg
     3901 aaggctttgg cggataattt tatcgtcttc gacaacggcc actttgatca tacatatctc
     3961 atatgatttt ttttaacgta tgttatattg gattcgatta caactggcac aagtgatagt
     4021 tttatatttt gcctgtaaac gtaacacgcg ttcccttgtt tggctcagag tgaatctcta
     4081 tttgcccgtt cgcttctaaa gctcttgttt tcatgttatg catcccattg ccgcctgcac
     4141 ggggatgggc gtcttcaaaa ccaacgccgt catcgcttaa tacgatggaa agccgatcac
     4201 cttttacgtg tgcggtaaga gtcaatgtct tacagcgact gtattttaag gcattggcgg
     4261 tggcctcttt aaaaatcaaa taaatatgac tacgccgctc catcgccaaa cgtaagccgg
     4321 ataacgaatc aatgttttct gtaataaact ccactccgga ctgctccgcc atatcttccg
     4381 aggattcttt gagccgaaga atcaattcgt ataacgaatc ccgacgcgga ttgacaagcc
     4441 atatgatatc gcgcatttct tcatacagcg atttggatat agcgtcaatt atagtaagat
     4501 tatgccgcac ctgctccgtc ttctccgaat gcatcaacgc tgtctgtgtc agataatgca
     4561 cttgggtcag acccgctcct atcgcatcat gaaaatcggc tgcaatttta tttctcaaac
     4621 gttcaacagc gagcatagcc tgaattcggc tataaacgat caacgaaatc aatccacctc
     4681 ccgctagcgt tacaagcata atgaaccacc acgttttcca atagggaggc tctataatga
     4741 tacgatatga acaaatttcg ctccaaaggc cgtattcatt agatgcacgc acctcaaatg
     4801 tataagtacc atcgcttaaa ttcggatatt gacaaaaatg atgttcggat gttacccatt
     4861 cgcgatcaat ggatgaaagc cgatattgat acacgacacg ctccggtgcg tgtagattga
     4921 gaccgacaaa atcaaatttt atataattct cattatatgg aagggaaatg acgtcggaag
     4981 ccgacttcgc aagtaattca ttatcgaacg cacgcacacg tgttatatgt acattcgggg
     5041 gcgtcggctg gcgcacctcc attcgtgggt cgtaatgcac ggcacctttg gtcgtaccaa
     5101 accacatcgt accatcgctg ttcttccgaa aggcaccctg atttaactca tcactgggca
     5161 atccttcgga tttggtgata acgttaattt caaaatgccc attttttatt tcgattcggt
     5221 taatgccctt gtgtgtagcg gcataataga tattctcctt gtactgtata acgccgtaaa
     5281 ctgtattatc cgacatgccg ttgcgtgtat taatcgtatc aattgttgag ttagaaaaaa
     5341 tggtaatacc accgttgttt gtcgaagccc atatatactg cgcggagtcc acataaacgt
     5401 tccatacatg atcatcagaa aggccttttt tccgatttat cgtatctacg atacctttat
     5461 tcacatgcat gacaccatag cgcgtcgcaa ggtagatttc atcatctgat acggcatgaa
     5521 tgcccgtaat aaaagcacgg ttccagcgat gtcctttcgg ataggaataa atttttcctt
     5581 tatccaaaac atccaatcct tcgcgatggc ctataaatac gctaccatcc gaacgctctc
     5641 gcatcacata tacccgattt tcagacagtc cttctgcttt tccatacaac tttttcgaac
     5701 cggagggtgt aatatctatg ataccgaaat tattagttcc gacaaaaaag ttctctctcg
     5761 atgttgaaag taagctccat atggtgagat caggaaaacc atttttttta ttccaatgtt
     5821 gccatttatt tttatacagc atggatacgc cgcccgtggc tgtaccgaaa taaatagctt
     5881 gacgcggatc ttcttcgata gccaaaacta cgggatcctg aagaccattt tcacgtcggt
     5941 acgatcggat acgtccaccg tcataaaagt ccaaaccgcc ccaggtaccc agtgcgatcc
     6001 atccattttc taaagtttcg atagcccaaa ccgaattatt agccagtcct tgatcttctc
     6061 cgatgtgcaa ccatttacca ttacgcaaat tataaacacc gtttccgata gtagtaaaaa
     6121 acacaccccc gtcttttatc cagcgcattg tttgcaccct ataatcagaa agcggtgcaa
     6181 gccacgtcaa actgttattt gttatttttg ccgtgccgcg gccatccaca gcgacatacc
     6241 attcattttc agcttcaacg tagataaatg atcgaatctt aggtatgtcc gaggacagcg
     6301 aagggaataa aacttcaaac gtatcattaa ggaactcata caatccgttg tcaaaacatc
     6361 cggcataaac tttattcttg tacgctttaa cgtccgaaat cacaatccct gtaaaaaaaa
     6421 tacgcgctga agccgaatcc agtatcgcat cggtaaaacg gactaatcct ttgtctgtgc
     6481 ctacccacaa tgcaccgtct gtatccgacg cgatcgtgcg caccgacata tcaggcaggc
     6541 cgcttcgttt atcatgtaag atcgcccgct gtcctatgat ctcaagcaac ccttggccat
     6601 tcgtagctgc aaaaatacga ccattcacat gctcatgcag ggatcgaatc ccacctggag
     6661 atgaaccatc ttcaagtcga atcgaataaa attcattacc atcccaacgc gccaatccgc
     6721 ccagcatccc aatccataaa tatcctttgg tatctcgcat aagcgctgtg acttgagatt
     6781 gaggtaagcc gtgatcggta gaaatatttc tgtgtatgtg cgtctgagcc aataagatcg
     6841 cagcggggta cggacatagt tttataaagc acaatatcaa tagtatgctc cacgcgcaat
     6901 gctttgaagg catcataaat aagacacgat atttgaaagt tgtagaatta tggccgcttg
     6961 cgaaccggta agtacactgt gaactccgaa ccttttccta cttcactcca cacttcgatc
     7021 tttccgtggt gccgctgaac aatcccgtac gcttccgtaa gaccaagccc acgtcccgac
     7081 cccacatcgc gtgttgtaaa aaacggatcg taaattttat ccataacggc cgaagacatg
     7141 ccgatgccat tgtcgcgaat cgaaacaaca acatattcgt tttgttcaac ggtctgtgtg
     7201 gatgtggata aatcaatctg accttctttt aatcctctat cctgtatagc ttgcaccgca
     7261 ttcgtgagaa tattgacgag cgcttgattt atttcggccg tattacagta tacggcaggg
     7321 aggccgttat attttttttc aattgtcaca aaatcatact gcttgataaa aagatcacaa
     7381 ataatatcaa gattgtcttc taaagaaatt aatttccaat cggattcatt gagattacta
     7441 aaacgtttaa ggttatcaat gattgcctta atacgcatgc cgcctaccat cgaaccctcc
     7501 agtgacggca tcatctcagc atgtaatttt ttaa
//
LOCUS       DAOQNQ010000153         7523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3694_length_7523_cov_5.615828, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000153 DAOQNQ010000000
VERSION     DAOQNQ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7523
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3694_length_7523_cov_5.615828"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PK754_09960"
     CDS             complement(<1..208)
                     /locus_tag="PK754_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNB09872.1"
                     /translation="MKDNRLQAQRSLPLSSWHIVFPNDTNPHGTMFGGKVMAIMDIQA
                     GIVATQYCHRMVVTASTEAVDFRNP"
     gene            complement(208..2988)
                     /locus_tag="PK754_09965"
     CDS             complement(208..2988)
                     /locus_tag="PK754_09965"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HNB09873.1"
                     /translation="MRRLSTILFVLILGYYAVSSYVSFYSELLWFQSMGYEEAFWRMY
                     MTEYIVGAVYFAIFWLVIGVNIWIASRMEAVYVSTTGGVYQQPFNLVAKPAKTIFFGI
                     LIFVSYIMSSAPAAKWMRILQYMHSESFGTADSVFQKDIGYYIYQLPFYQSIVSWSYG
                     VVIVAMIAVLATYLYRKAIYIEPQGIRLADVVKRHLMILGAMLLLIYAVDNYFSAYNI
                     LYKDNGTVVGASYTDVHAVLLGYRVMIAVAILGAIAVLWGAVQATWRFAVIGGVAQFA
                     VGFVMLNVYPGLIHRFVVTPNALEKEKPYIEENIRQTRRAYELDKITEQDFNYELSLK
                     ASDLKANSSTIKNITLWDYRPIKDSYSQLQEIRPYYNFYDVDIDRYMINGEYRQVMLA
                     GREMNLDKIGSSENWINKTFIYTHGHGIVMSPVNVVTQEGQPEFFIKNIPPELAVNIK
                     IDRPEIYFGELMNESDYVIVKTSKEEFDYPLGESNQHTMYKEESGVSIGSFARKMLFA
                     IRFNKFNLLLNDYIQAQSKVLYYRNIRDRVEKIAPYIRWDRDPYLVAENGRLYWMMDG
                     YTITDQYPYSAMSEERVGSGAFARKRYFNYIRNSVKVVIDAYNGKTDLYAFQPDSDPL
                     IRVYGKMFKGVFKEYATMPDELKKHVRYPQDLFDIQASHFASYHVSDANVFFNKEDVW
                     NVAQEKYGDDVQTMESYYMIMRLPGETKEEFITLLPFTPNNKSNMIAWFCVRSDGDNY
                     GKLLVYRFPKTELVYGPMQVESRIDQTPEISEKLTLWNQQGSRVTRGNMLVVPINNSL
                     LYVEPLYLQSNQSRLPELKKVIVAYGNSIFMEDNLEIGLEKIFGGNYGDFGTSSTVAP
                     MSENKATTDNVSSGLSSDQSRALRELSRSAMQNYNNAQDALKRGDWAKYGEFLERLKR
                     DLDKLNEQSQKF"
     gene            complement(3030..4379)
                     /locus_tag="PK754_09970"
     CDS             complement(3030..4379)
                     /locus_tag="PK754_09970"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNB09874.1"
                     /translation="MYKRSSVYIFLSLILLLNGCVPQYGELKLPPRHIIIKYCWETFE
                     LGLKDKNDFVKTSTLTTLGRIGSASAAETIASADLGVKPQVTRTAVATLAQMHDSAAF
                     QALLRFRGHSDFFVRENVAMGMVRMRDLYGDTLVARVLKRMYLYVDSIESDTMFYEKS
                     EIQQERREIKTKLAIAISSVDPRFPDPQIALAYRNAPLAARVTLVNFIGAVKPPNALT
                     WLSPFMIDSSTYVRSKTAEALGKIASPQAYDMLRGMLRTQRREEVIVNAAIALMPADE
                     ARAVEVLIQAMDTYDEDTQSNILLALGEAKLPESRAKILPLVKSAVTGKGDWVRIGAI
                     GALGLIKDTTTLPLIEAALEDPVDEVREIAVGVLAQMKGETMVDRLKAWAKDDQYSMR
                     SVALAGLGSLRDPVIVEKTVYPVLYNTMKFDPEMMVRVRAAFTLLNIINDRKYTKGI"
     gene            4520..5263
                     /locus_tag="PK754_09975"
     CDS             4520..5263
                     /locus_tag="PK754_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB09875.1"
                     /translation="MNSEGTKGSPFPRSNRILIVEDDPDTIKILRLYFIKEKFEVDVA
                     GDGEEGLSILRQHHHDIVLSDVMMPKMDGYKLCEAIRSDDTIKMTPIVLVTAKVELSD
                     KLTGLEKGADAYLTKPYNLTELRAQIASLLKLRELRLALTNREKEIERIRTLEQTLIA
                     ISHHINNAIAPISGRAQLTDPADVEQVRKLIDVASIGCRRISRTINLLSDVVSAMKQA
                     SNDQAFDLNKTTINELLEKFKSNGDTDLQ"
     gene            5360..6793
                     /gene="dnaB"
                     /locus_tag="PK754_09980"
     CDS             5360..6793
                     /gene="dnaB"
                     /locus_tag="PK754_09980"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNB09876.1"
                     /translation="MSVQEDQINGKILDYPGRVPPQATEVEEAILGAILIDEAAFTSI
                     MDFLDESCFYKESHRIIYGVMKTLYLRSEPIDALTVTEELRRSNQLERIGGAYFITGL
                     TNKVPSASNIATYTKIVLDKSRLRKLITVGGQIVNMAFQETEDVDTLIDTVEKQIFDI
                     AQDKASQQFRPLNAILHDAFEQMDNYYNRPGGLTGVPSGFQSIDHITNGFQASNLIIV
                     AARPGMGKTALVLNVARHAAVLHQKAVGIFSLEMSSTDLVTRLLCAEAEVDAQRLRSG
                     NLPDKDYRKLHTSVNNLHKANIFIDETAGLNILDLRARARRLKAEHNVELLIIDYLQL
                     INASVRSGANRQEEVALISRSLKALAKELSIPVIALSQLSRAVEQRGGDKRPQLSDLR
                     DSGSIEQDADVVMFIHREDYYNTAQDAFNQSMVTEAEIIIAKHRNGPTDTAKLGWARK
                     FTKFVPMDTAHTGIVPPPSSHDVEPAF"
     gene            6797..>7523
                     /locus_tag="PK754_09985"
     CDS             6797..>7523
                     /locus_tag="PK754_09985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB09877.1"
                     /translation="MLPASPLVSVVILNYNGRRFTDTLFESLRRTTYPHVEWVMVDNA
                     SSDDSVSYTREKFPWVRIIETGANLGYTGGNNVGILQSKGAYVVLLNNDVEADPDWLT
                     HLVNAAEADASIAALQPKLQSMINKGFFEYAGASGGFIDPWGYPFLRGRIFDTIEKDH
                     GQYDDVRDIFWASGAALFLRRSALDEIGLLDEDFFMHFEEIDLCWRLHWAGYRIQVIP
                     QSRIYHYVSASLPAANLRKLYWNH"
BASE COUNT         1963 a         1912 c         1730 g         1918 t
ORIGIN      
        1 ccggattgcg aaaatccacg gcttcggtgg acgccgtcac caccatgcga tgacaatatt
       61 gtgtcgcaac aatgccggcc tgaatatcca tgatcgccat gaccttaccg ccaaacatcg
      121 ttccgtgcgg attggtatca ttgggaaata caatatgcca gctgctgagc ggcaatgaac
      181 gttgggcttg caaacggtta tccttcatta gaatttttga ctttgttcgt tgagtttatc
      241 caaatcacgc ttgagacgct caaggaattc gccatactta gcccaatcac cgcgtttgag
      301 cgcatcttgt gcgttattat aattttgcat cgccgagcgt gaaagttcac gcagagcccg
      361 cgattgatcc gacgacaagc cgcttgatac gttatcggtc gttgctttat tctcagacat
      421 cggtgccacg gtactcgaag ttccgaagtc gccgtaatta ccaccgaaaa ttttttccaa
      481 tccgatttcc agattgtctt ccataaaaat actgttgccg taggccacga tgactttttt
      541 cagttccggc agacggcttt gattggactg cagatacagc ggctcaacgt agagcagtga
      601 attattgatc ggaacgacga gcatattgcc gcgcgtcacc cgtgaacctt gctgattcca
      661 cagcgtaagt ttttctgaaa tctccggcgt ttgatctata cggctttcaa cctgcatagg
      721 accgtacacc agctccgttt tgggaaaacg gtacaccaga agttttccgt aattgtcccc
      781 gtcactacgc acacaaaacc aagcgatcat attggatttg ttattgggcg taaaaggcaa
      841 caacgtgata aactcttctt tggtttcgcc cggaaggcgc atgatcatgt aataactttc
      901 catggtctgc acatcatcgc cgtatttttc ctgagcgaca ttccacacat cttccttatt
      961 aaaaaacaca ttagcatccg aaacgtgata cgatgcaaaa tgggaagcct gaatgtcaaa
     1021 cagatcctgc ggataacgca catgtttttt gagttcgtcc ggcatggtag cgtactcttt
     1081 gaacacccct ttgaacattt ttccgtaaac acggatcagc ggatcgctgt cgggttgaaa
     1141 tgcatacaga tcggttttgc cgttgtacgc atcaatcacg actttgacgg aattgcggat
     1201 ataattgaaa tagcgtttgc gtgcaaaagc cccggaaccc actctttctt ccgacatagc
     1261 tgagtacggg tattgatccg tgatcgtata tccatccatc atccagtaaa gacgtccgtt
     1321 ctccgccacg aggtacggat cgcgatccca ccggatatac ggcgcgattt tttcgacgcg
     1381 atcgcggata ttacgatagt ataatacttt actttgcgcc tgaatatagt cgttgagcag
     1441 gagattaaat ttgttaaaac gaatcgcaaa aagcatctta cgggcaaagc tgccgatact
     1501 tacaccggat tcttccttgt acatcgtatg ctgattggat tcgcccaaag gatagtcaaa
     1561 ttcttctttc gaagttttga cgatcacgta atccgattcg ttcatcagtt caccgaaata
     1621 aatttccggg cgatctattt tgatattcac cgctaattca ggcggaatat ttttgataaa
     1681 aaactccggc tgaccttctt gtgtaacgac attgaccgga ctcatgacga taccgtggcc
     1741 gtgggtgtag ataaaggtct tattgatcca attttcactg gaaccgattt tgtcgagatt
     1801 catttcacgt ccggccaaca taacctgacg gtattcgccg ttgatcatgt agcgatcaat
     1861 gtccacgtca taaaaattgt aatacggacg tatctcctga agctgcgaat acgaatcttt
     1921 gattggacga taatcccaca acgtgatatt tttgatcgtg gacgagttgg ctttcaagtc
     1981 ggacgctttg agcgataatt cgtagttaaa atcctgttcg gtgattttat ccaattcgta
     2041 cgcgcgacgc gtttggcgaa tattttcttc gatataaggt ttttcttttt cgagcgcgtt
     2101 gggagtgaca acaaaacgat gaatcagccc ggggtacacg ttgagcatga caaagccgac
     2161 ggcaaattgg gcaacgccgc cgatcaccgc aaaccgccac gtcgcttgga ccgcgcccca
     2221 taatacagcg atcgcgccca gtatggcgac ggcaatcatc acacgataac caagcagaac
     2281 ggcatgtaca tccgtatacg atgcgccgac taccgttccg ttatctttgt acaggatatt
     2341 gtacgccgaa aaataattat ccacggcata gatcaaaagc aacatggcgc ccagtatcat
     2401 cagatggcgt ttcacgacat ccgcaagacg aatcccctgc ggttcgatat aaatcgcttt
     2461 acggtaaaga tacgtggcca gtacagcgat catcgcgacg atcaccacgc catacgacca
     2521 tgaaacgata ctctgataaa atggcagttg gtaaatataa taaccgatgt ctttttgaaa
     2581 cacactgtcc gccgtaccga agctttcact atgcatgtat tgcaaaatgc gcatccactt
     2641 ggcagcaggc gcactggaca tgatatagct tacaaagatc aatatgccga aaaatattgt
     2701 tttggccggt ttcgcaacaa gattgaaagg ctgctgatac acaccgccgg tcgttgaaac
     2761 gtacaccgcc tccattcggg aagcgatcca tatattgaca ccgatcacaa gccaaaatat
     2821 cgcgaagtat accgcaccga caatatattc ggtcatgtac attcgccaaa aggcctcttc
     2881 atatcccatg gactgaaacc acaacagctc gctgtaaaaa ctgacgtaac tggatacggc
     2941 atagtaaccg agtatcaata cgaaaaggat ggtggacaaa cgacgcatat gcgttgatta
     3001 gctccttgtt aggttagcaa tacttagctt caaattcctt tggtgtactt acgatcgttg
     3061 atgatattaa gcagcgtaaa cgcggcccgc actcggacca tcatttccgg atcaaacttc
     3121 atggtattat acaaaaccgg atacacggtt ttttcgacaa tgacgggatc gcgcagggaa
     3181 cctaatccgg cgagggcgac ggaacgcatg gagtactgat cgtcttttgc ccaggctttg
     3241 agccgatcaa ccatcgtttc gcctttcatc tgagccaaca ctccgacggc aatttcgcgg
     3301 acttcgtcca ccggatcttc cagcgccgct tcgatgagcg gcagcgtggt cgtatctttg
     3361 atcaatccca gagcgccgat ggcgccgatg cgcacccaat cgcctttacc tgtgacggcc
     3421 gattttacca atggtaaaat tttagcacgg gattcgggca atttggcctc accgagagcg
     3481 agcaagatat tactttgcgt atcttcgtca taggtatcca tcgcctgtat caaaacttcg
     3541 acggcgcgcg cttcgtcggc cggcatcaag gcgatcgccg cattgactat cacttcttca
     3601 cgccgttgcg ttcgcaacat gccccgcagc atatcatagg cttgcggtga agcgattttt
     3661 cctaaagcct cggccgtttt ggaacgcacg taggtggaag aatctatcat aaacgggctc
     3721 aaccatgtca gcgcattggg tggcttgacg gcgccgataa aattgaccag tgtgacacgc
     3781 gccgccagcg gtgcgttgcg gtaagccagc gcaatctgag gatcagggaa tcgcggatcg
     3841 accgatgaaa tagctatggc cagtttggtt ttgatctcac gccgctcttg ctgaatctcg
     3901 gatttttcgt agaacatcgt gtcgctttcg atcgaatcca cgtatagata catacgcttg
     3961 agtacgcggg ccactaaggt gtcaccgtac aaatcccgca tgcgtaccat acccatcgcg
     4021 acattttcgc gaacaaaaaa atcagaatgg ccacggaacc gaagcaaagc ttgaaatgcc
     4081 gcactgtcgt gcatctgcgc caacgttgcg acggctgtgc gtgtcacttg cggtttcaca
     4141 ccgagatccg ccgatgcgat ggtttccgcc gccgaggctg aacctatacg gcccaaggtc
     4201 gtcagcgtgg atgttttgac aaaatcattt ttgtctttaa gaccaagctc aaatgtttcc
     4261 caacaatatt ttataataat atggcgcggc ggcaacttga gttcaccgta ctgcggtacg
     4321 catccgttta acaacaaaat gaggctcaaa aaaatatata cggacgatcg tttgtacacg
     4381 tttttactcc ctccgtctgc gcataaaaaa ggcttgcgca tgggtaattt tctgtagtat
     4441 gttgtcctgc cccatagggc gcacaattga aaatcaaagt ataaacaatt tcctcaaaag
     4501 tcaattttat cgggcgtgca tgaactctga aggtactaag ggatcgcctt ttccgcgcag
     4561 taaccggata ttgatcgtcg aagacgaccc cgacacgata aaaatccttc gtttgtattt
     4621 catcaaagaa aaatttgaag ttgatgtagc gggcgatggc gaagaaggtt tgtcaatttt
     4681 acggcaacat caccatgata tcgtactgag cgacgtcatg atgcccaaaa tggacggcta
     4741 caaattgtgc gaagccattc gcagcgatga tacgatcaaa atgacaccga tcgtgcttgt
     4801 taccgccaaa gtcgaattga gcgataaact caccggcctg gaaaaaggcg cggatgcgta
     4861 tctgaccaaa ccatacaacc ttaccgaact ccgcgctcaa atcgcgtcgc tgctcaaact
     4921 tcgcgaactt cgtctggctt tgaccaaccg tgaaaaagaa atcgaacgca ttcgcacctt
     4981 ggaacaaaca ttgatcgcga tttcccatca tatcaataat gccatcgcgc cgatttcagg
     5041 acgtgcacag ctcaccgatc ccgccgacgt cgaacaagtg cgcaaactga tcgatgtggc
     5101 ctccatagga tgccgccgca tttcccgtac gatcaatctt ctgagcgacg tggtctctgc
     5161 gatgaagcaa gcctccaacg atcaagcgtt tgacctcaat aaaacgacga tcaacgagct
     5221 gctcgaaaaa ttcaaatcaa acggcgatac ggacttgcaa tagtccattt tttgattaac
     5281 ttcactcccc gcaaaactaa gatttagact gagttttgct gttattattt gagtcaccta
     5341 accatttttt ataaaaaaca tgtccgtaca ggaagaccaa atcaacggta agattctcga
     5401 ctatcccggg cgcgtaccac cgcaggccac ggaagtcgaa gaagctattc tcggcgccat
     5461 tttgattgat gaagccgcat ttacctcgat catggatttt ttggatgaat cctgtttcta
     5521 caaagaatcc caccgcatca tatacggcgt gatgaaaaca ttgtatctgc ggagcgagcc
     5581 gatcgatgcg cttaccgtaa ccgaggaact tcgacgctcc aaccaactgg aacgcatcgg
     5641 cggagcatac tttattacag gtttgaccaa taaggtgccc agcgcttcca atattgctac
     5701 ctacaccaaa attgttcttg ataaatcccg tttacgtaaa ctgattactg tcggcggaca
     5761 aatcgtcaat atggcgtttc aggaaaccga agacgtggat acactgattg acaccgtcga
     5821 aaaacaaatt tttgatattg ctcaggataa ggccagccaa cagtttcgtc cgctcaatgc
     5881 gatcttgcac gatgcttttg agcaaatgga taattattac aatcgcccgg gggggctgac
     5941 cggcgttccg tcaggttttc aaagtattga ccatatcacc aacggttttc aagcttccaa
     6001 tctgatcatc gttgccgcgc gccccggtat gggaaaaacg gccctggtac tcaatgtcgc
     6061 ccggcatgca gcggttttgc atcaaaaagc cgtaggtatt ttttcgctcg aaatgtcttc
     6121 gaccgatctt gtgacgcgtt tgctttgcgc ggaagcggaa gtggacgccc aacgcttgcg
     6181 cagcggcaat ttgccggata aggactatcg caaactgcat acgagtgtca ataatctgca
     6241 taaggcaaat atctttattg acgaaacggc cggtctcaac attcttgatt tgcgtgcgcg
     6301 ggcacgacgt ctcaaagcag aacataatgt cgaacttctt attatcgatt atctccagct
     6361 gatcaatgcg tcggtgcgtt cgggtgccaa tcgtcaggaa gaagtggcct tgatctcccg
     6421 ttcgctgaaa gcgttggcta aagaattatc catacccgtg attgctcttt cacagctttc
     6481 gcgcgccgtc gagcagcgcg gaggggacaa gcgcccgcaa ctttccgatt tgcgcgattc
     6541 gggttctatc gaacaggatg cggacgtcgt tatgttcatc catcgcgaag attattacaa
     6601 tacggcacaa gatgccttca atcagtccat ggttacggaa gccgagatta tcatcgccaa
     6661 acaccgcaac gggccgaccg acacggccaa actcggatgg gcgcgcaagt ttaccaaatt
     6721 tgtcccgatg gacacggcgc acacaggcat cgtaccgccg ccgtcttctc acgatgtcga
     6781 accggcattc tgatccatgt taccagcatc cccgcttgtc tccgtcgtta tcctcaacta
     6841 taatggccgt cgcttcaccg atacgctttt cgaatcgctc cggcgtacca cctatcccca
     6901 tgtcgaatgg gttatggtgg ataatgcatc cagcgacgac agcgtatcct acacacgcga
     6961 aaaatttccc tgggttcgca tcatagaaac aggcgctaat ctcggctata cgggcggcaa
     7021 taacgtcggc atccttcaaa gtaaaggcgc ctacgtggta ctcctcaata acgacgtcga
     7081 agccgatccg gattggctca cgcatttggt caacgccgct gaggccgatg catcgattgc
     7141 tgcgctccag cccaaattgc aatccatgat caacaaaggt ttttttgaat acgccggggc
     7201 cagcggtggt tttattgatc cctggggata tccctttttg cgcggccgga tatttgatac
     7261 catcgaaaag gatcacggac aatacgatga cgtgcgggat attttctggg ccagcggtgc
     7321 ggctttgttt ctacgccgtt cggcgctgga tgaaatcggc ttgctggatg aagacttttt
     7381 tatgcacttt gaagagattg atttatgctg gcgattgcat tgggcaggtt atcgcataca
     7441 ggttattccg caaagccgga tatatcatta tgtcagcgct tctctgcctg cggccaatct
     7501 gcgcaagttg tattggaatc acc
//
LOCUS       DAOQNQ010000154         7511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3704_length_7511_cov_6.407725, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000154 DAOQNQ010000000
VERSION     DAOQNQ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7511
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3704_length_7511_cov_6.407725"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PK754_09990"
     CDS             <1..555
                     /locus_tag="PK754_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M14"
                     /protein_id="HNB09878.1"
                     /translation="PYAFIVPQQWTSVLERLEAHGIVTEKLDKDRDIKVISYRFSNAK
                     WSENPFEGRLSVNYKTSETEETRRFRAGDRIVKLNQRTARVIMHLLEPRAPDALVQWG
                     FFNAIFEQKEYGETYKLELLARDMLAKDSALRAEFEKRVKEDKNFTGYNRLNWFYERS
                     PWWDKNVNLYPVGKILTAEDISGF"
     gene            650..1360
                     /locus_tag="PK754_09995"
     CDS             650..1360
                     /locus_tag="PK754_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09879.1"
                     /translation="MKQRLFFGMMLLATWYNTAFAQSGNPVYGASFYRLWTSSDKISE
                     YAYALRVESSKYGYGVFHWGTRSKDPNESYKGPSSDPFDAAHMTGWFLRPNIWPFGAR
                     KIPVYAHLGYTSRNYNSDVIDEGLNGDLSSHDWSFGLFTKNEYQIKQRVDLAPEIGVY
                     HITRTAKPYGDLSAGDSRGWVYEANLRIGVRLISGLSLITDLGWSDEFSTNGKHSWLQ
                     GEYPGKINAGAGIAYYIR"
     gene            1532..1918
                     /locus_tag="PK754_10000"
     CDS             1532..1918
                     /locus_tag="PK754_10000"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNB09880.1"
                     /translation="MVRTIFRAKIHRARVTQLDLNYHGSITIDQDLLDLVDIWHNEKV
                     QIVNANNGARLETYVIAGKRGSRVIGLNGPAARLAMPGDVISIIAYGAAEESEARAFH
                     PKVVLLNQNNEVVESHTIPSHDDVNR"
     gene            1969..3246
                     /locus_tag="PK754_10005"
     CDS             1969..3246
                     /locus_tag="PK754_10005"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNB09881.1"
                     /translation="MQDIRTTLTYLYDLQFFGIKFGLENTRALLQFVGNPHKKFPVIH
                     VAGTNGKGSVCAMLASIFEAAGLRCGLYTSPHIVHFSERLRINNIEISENDIVRLTRL
                     LQPEIDRLQCTFFEATTVMAFQYFAEQHVDIAVIETGLGGRLDATNVVEPEVSVITTI
                     DFDHMAYLGDTLEKIASEKAGIIKPDKPVIVGNIPSEAFSVMQKKAHENRSPLYVLGT
                     HITIQNSEVGIDGGCMDAQWRFSDSQGTITKINIPLIGEHQLDNAALAVAAARLQTRF
                     SVSDDDIRKGIRAVIWKGRCFIKRKRPWVIVDAAHNPAGMRSLARSIALLHKEITGQK
                     ILMIGMLSDKDSRNALHEIVPLFDRVVAVTPPNPRALPADALAALIHQTGKPVEVIPD
                     TVSAYDTTLQKLKADDLLVVTGSFYVLQDILLR"
     gene            complement(3238..3597)
                     /locus_tag="PK754_10010"
     CDS             complement(3238..3597)
                     /locus_tag="PK754_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNB09882.1"
                     /translation="MAKFEHHIFICTNTREDGHPRGSCNTGGTNNLQKLFNEALKKYD
                     LKGKVRANKAGCLDQCEHGPNVVIYPDNVWYGFVKDGDVENIVRAMAEGNHASDRILP
                     DSCLNTSACPHRKNGSA"
     gene            complement(3614..4249)
                     /locus_tag="PK754_10015"
     CDS             complement(3614..4249)
                     /locus_tag="PK754_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNB09883.1"
                     /translation="MHSMTMYDLIERLDEEEEEKETEEPQKEDPLGKKMAEFFLQSRT
                     ILLFEQISPKVTRRVITQLLFLNEQDSKKEIKIFINSPGGVADDGLAIYDLIKLIDAP
                     VKTIVCGLAASAAAIILVAAKKENRLALPHSRIMIHQPLGGVRGSTKDIEISTQQIVR
                     LKQELNEILARETGQPVEKVAQDTNRDYWFTADEAKAYGMISKTVTGLSKI"
     gene            complement(4266..4970)
                     /locus_tag="PK754_10020"
     CDS             complement(4266..4970)
                     /locus_tag="PK754_10020"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF015685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNB09884.1"
                     /translation="MKRLGILLDAVVAIRQMGGGKTPDPLHVISMLEMAGVDSIVYTM
                     MQTGAAEERDLRMLKETIHSHFNLRLVPTMENAQAAIQAKAEMVTMIDRDSTSIRSVN
                     AVEKEALLAPIVSFLRTNGIVVNMLVDTDANQLRTAARMGCDYVELNTDKYTGAASLT
                     LMEQELENMKAMAMAAKKLTLSVTLSGHINHNNIRELLDIEEIEEINIGHSVFARALF
                     VGLDQAVRDFATLIKK"
     gene            5184..6434
                     /locus_tag="PK754_10025"
     CDS             5184..6434
                     /locus_tag="PK754_10025"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNB09885.1"
                     /translation="MISFNGILIILAVILVLWLIDKYVASGLRTLVRDWKKSHIIIRS
                     FWVANVLMYVLVTTGMLFFMLRLGPMPLRATIMGLFVALLVPKLVLVALLLAEDATRW
                     LRKAWSVVRRTKTKEETFLPSRRKFIAQIGFGLAAIPFGSILYGITRGKYDFTVHRTD
                     VFIKDLPSVFDGFTITQLSDIHAGSFDDADAVKRGVDLANAQRSDLLVFTGDMVNNRA
                     EELTPWLNIFKTLNADYGKYATLGNHDYGDYVSWPSPEAKAENLAWLQSMHAEMGFTM
                     LNNAHTVISKNDAHLALVGVENWGLPPWPQKGDLKKALEGIEPAAPKILLSHDPTHFE
                     GEVLQHASRIDLTLSGHTHGMQFGVEIPGFRWSPVQYRYPRWAGLYTENGRALYVNRG
                     FGFIGLPGRVGIWPEITVITLRCG"
     gene            complement(6523..6801)
                     /locus_tag="PK754_10030"
     CDS             complement(6523..6801)
                     /locus_tag="PK754_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010853241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HNB09886.1"
                     /translation="MKQKELSKSAEKELSQLTDQELLVEAKKMKSFSINNALFVGFMA
                     GIIIYSVAKNAWGFFTLIPVYFIYKLVNDPKNIRSKVLERLLKERNLK"
     gene            complement(7131..7430)
                     /locus_tag="PK754_10035"
     CDS             complement(7131..7430)
                     /locus_tag="PK754_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HNB09887.1"
                     /translation="MNGKIKYEFSAKTWQHPSPNGWHFVSLPIKMAKEIRENFKRQEE
                     GWGRLKATAKIGNTEWETAIWFDTKHKTYILPLKSEIRKKEKLELNKNIKAMIWV"
BASE COUNT         1926 a         1737 c         1709 g         2139 t
ORIGIN      
        1 ccgtatgcat tcatcgttcc ccagcagtgg acatccgtat tggaaagact ggaagcgcac
       61 gggatcgtaa cggaaaaatt agacaaagac cgcgatatca aagtgatctc ataccgattc
      121 tccaacgcca aatggtctga aaatccgttt gaaggccgtt tgtcggtaaa ttataaaacc
      181 tccgaaaccg aagaaacgcg ccgcttcaga gccggtgacc ggattgtgaa actcaatcaa
      241 cgcacagcac gtgtgatcat gcatttactc gaaccacgcg ctcctgatgc cttagtccaa
      301 tggggatttt ttaatgcgat cttcgagcaa aaagaatacg gcgaaaccta caaactggaa
      361 ctgctagccc gcgatatgct ggccaaagac agcgcgctgc gcgcagagtt tgaaaaacgc
      421 gtcaaagagg ataaaaactt taccggatat aatcggctca attggtttta tgaacgctct
      481 ccatggtggg ataaaaatgt aaatctgtac ccggtgggta aaatattaac cgccgaggat
      541 atttccggat tttaatgagg cctacacaaa tgtgtagtgt ttgaaacata gttcgcttta
      601 ttgcgttgtg gattgcacat aacactataa caacaaataa aaggaacaca tgaaacaacg
      661 tctttttttc ggtatgatgc ttttggcaac ttggtataat acggcatttg cacagtccgg
      721 taatcccgtg tacggtgcat cgttttatcg cctatggacg agcagcgaca aaatcagtga
      781 atacgcatac gccttgcgcg tggaatcatc gaaatacggt tatggagttt ttcattgggg
      841 aacgcgatcc aaagatccta atgaatctta taaaggccca tcctctgatc cgttcgatgc
      901 ggcccacatg accggatggt ttttaagacc caatatttgg ccattcggcg cacgaaagat
      961 acccgtttat gcgcatttgg gttatacgtc acgaaactat aattcggatg tgattgatga
     1021 agggttgaat ggcgatttgt cctcgcatga ttggtctttc ggattattta ccaaaaatga
     1081 atatcagata aagcagcgcg tggatttggc tcctgagatt ggcgtgtatc acatcacacg
     1141 aaccgccaaa ccttacggtg atttgtcagc aggagacagc cggggttggg tctatgaagc
     1201 gaatttaaga atcggcgttc gtcttataag cgggcttagt ctgatcactg atcttggttg
     1261 gtcggacgaa ttttctacaa acggaaaaca cagttggctt cagggcgaat atccgggtaa
     1321 aatcaatgcc ggtgccggta tcgcctacta tatccgataa accgcaatag ttgactttga
     1381 aaaaccatcg cataagtctc gtatagatac gaaagctcat gagatggttt ttatcttata
     1441 ccgatgaaat attcattgcc cgattgcgtt taagccatta tattgccgcg caatttttga
     1501 taacttttga aaataaaata agtaaaaacc gatggtccgt acgatatttc gcgctaaaat
     1561 tcaccgtgcc cgtgtcactc aacttgatct caactatcac ggtagtatca ctattgatca
     1621 ggatttgctg gatctcgtag atatttggca taatgaaaaa gtgcagatcg ttaatgccaa
     1681 taacggcgcc cgtttggaaa cgtacgtgat cgccggaaaa cgcggaagcc gcgttatcgg
     1741 tctcaacggg ccggccgctc gtttagccat gcccggtgac gtgatttcca tcatagctta
     1801 tggcgcggcg gaagaatccg aagcacgggc ttttcatcct aaagtagtgc ttctcaatca
     1861 aaataatgaa gtcgtcgaat cgcatacgat cccctcgcac gatgatgtaa accgttaaaa
     1921 cgcggtatcc cgaccttgtt ttttcatata tagtgacgtc acacgcgcat gcaggacatc
     1981 cgaaccaccc ttacgtatct gtacgacctc cagtttttcg gtataaagtt cggcctcgaa
     2041 aatacccgcg ctcttttgca gtttgtcggc aatcctcata aaaaattccc tgtcattcat
     2101 gtggccggta ccaacggaaa aggttccgtc tgtgcgatgc tggcttccat atttgaagcc
     2161 gccggtttac gttgcgggtt atacacgtcg ccgcatatcg ttcatttttc agaacgcctc
     2221 cgcatcaata atattgaaat ctctgaaaac gatatcgttc gattgacgcg tcttttacaa
     2281 ccggaaattg atcgtttgca atgtacgttt tttgaagcga cgaccgttat ggcctttcag
     2341 tattttgcag agcagcatgt ggatatcgcc gtcatcgaaa caggccttgg cggacgcctt
     2401 gatgcaacca acgtcgtcga acccgaagta tccgtcatca ctacgattga ttttgatcac
     2461 atggcttacc tcggtgatac gttggaaaaa atcgcctccg aaaaagccgg aattatcaaa
     2521 ccggacaaac ccgtcatcgt aggcaacata ccgtccgaag cattctccgt gatgcaaaaa
     2581 aaagcgcacg aaaaccgatc gcctctatat gtgctgggta cacatataac aattcaaaat
     2641 tctgaagtcg gtattgatgg cggatgcatg gacgcgcagt ggcgattttc tgatagtcag
     2701 ggcacgatca caaaaatcaa tatacctttg atcggtgaac atcagttgga caatgccgca
     2761 ttggccgtgg ccgccgcacg attacaaacc cgtttttccg tttcggatga cgatatccgt
     2821 aaaggaatac gcgcggtgat ctggaaaggc cgttgtttta taaaacgcaa acggccgtgg
     2881 gttatcgttg acgcggcgca taatccggca ggtatgcgtt cactggcccg gtctatagct
     2941 ttactgcata aagaaattac cggtcagaaa atactgatga tcggaatgct ttccgacaaa
     3001 gacagccgaa acgctttgca tgaaatcgta ccgctgtttg atcgtgtggt cgcagtgaca
     3061 ccgcccaacc cccgcgctct accggccgat gcactcgccg cgttgattca tcaaacagga
     3121 aaaccggtgg aagtgatccc cgatacggtg tcggcctacg ataccacact acaaaaacta
     3181 aaagccgatg accttttggt ggtcaccggc tcattctacg tcttacagga tattctttta
     3241 cgctgatccg ttttttcggt gcggacatgc cgacgtatta agacagctgt ccggcaaaat
     3301 ccggtcggac gcgtggttgc cttcggccat agcacgaacg atattttcca catcgccgtc
     3361 ctttacaaaa ccataccaca cattatcggg ataaatcacg acgttagggc cgtgttcgca
     3421 ttgatccaga cagccggctt tattggcgcg cacttttcct ttgaggtcgt attttttcag
     3481 cgcctcatta aaaagttttt gcagattgtt cgttccgccg gtattacaac ttccgcgcgg
     3541 atgtccgtct tcccgcgtgt tggtacaaat aaaaatatga tgttcaaatt tagccatgtc
     3601 gtattctctc ctctcagatt ttgctgagac cggttacggt ttttgaaatc atgccatacg
     3661 ctttggcttc gtcggcggta aaccagtaat cgcgattggt atcctgcgca actttttcga
     3721 cgggttgccc ggtttcacgc gctaaaattt cgttgagctc ttgtttgagg cgaacgattt
     3781 gctgcgtact gatttcaatg tctttggtgg aaccgcgcac accgccgagc ggttggtgaa
     3841 tcatgatgcg ggaatgcggc aaggccaaac ggttttcttt tttggccgca acaaggatga
     3901 tcgcggctgc actggcagcc aatccgcaga cgatggtttt gaccggtgcg tcaatcaatt
     3961 tgatcaaatc ataaatagcc aaaccgtcat ccgctacacc gccgggtgaa ttgatgaaaa
     4021 ttttgatttc ttttttggaa tcctgttcgt tgagaaacaa aagctgcgtg atcacacggc
     4081 gcgtgacttt ggggctgatc tgttcaaaaa gcaaaatcgt acgcgattgc agaaaaaatt
     4141 ccgccatctt ttttccgagt ggatcttctt tttgcggctc ttcggtttct ttttcttctt
     4201 cctcttcgtc taaacgttcg atgagatcgt acatcgtcat agaatgcata tatactccta
     4261 ttggattatt ttttgatgag tgttgcaaaa tcgcgcacgg cctgatcgag gcccacaaac
     4321 agcgcccgcg caaaaacaga gtgaccgata ttgatttctt cgatttcttc gatgtccaga
     4381 agctcgcgga tattattgtg attgatgtga cctgaaagcg taaccgatag cgtcagtttt
     4441 ttggcggcca tcgccatcgc tttcatgttt tcgagttcct gctccatcag cgtcagactc
     4501 gcagcgcccg tgtatttatc ggtattgagc tcgacgtagt cgcaacccat gcgggccgcc
     4561 gtgcgcaatt gattggcatc ggtatctacg agcatgttta caacgatacc gttcgttctc
     4621 agaaaactta cgatcggagc cagtagggct tctttttcaa cggcattgac actgcgaatg
     4681 gaagtactgt cacggtcaat catggtcacc atctccgctt tggcctgaat ggcggcctgc
     4741 gcgttttcca tggtcggtac caaacgcagg ttaaaatgcg aatggatggt ttctttgagc
     4801 atgcgcagat cgcgctcttc tgccgcgccc gtctgcatca tggtatatac gatactatcc
     4861 acgccggcca tttccagcat ggatattaca tgcaatggat cgggcgtttt gccgccgccc
     4921 atctgacgaa tggctactac ggcatccagc aatattccaa gtcgtttcac gctgtcattc
     4981 tccgaaaaat tcgataatta ccgctaaaga ttgataaatc ctgcgttaat ttcaacgcaa
     5041 attgcatacg gaacgctttt gttttttaga gagaacctct atattgattt acgcagcgcg
     5101 gcgtattaac tataaatgca ccgcaaccga aaccctactt caccgtatta tgttttaatt
     5161 cgtatgctta tggagaatat ttgatgattt cgtttaacgg aatattgatc attttagccg
     5221 tcatcctcgt tttgtggttg attgataagt atgttgcgtc cggtttgcgt acacttgtta
     5281 gggattggaa aaaatcacac atcatcatcc gcagcttttg ggtggccaat gtgcttatgt
     5341 acgtccttgt gacgaccggc atgttgtttt ttatgcttcg cttagggcct atgccgctgc
     5401 gtgcgacgat catgggatta tttgtcgcct tgctcgtacc aaagcttgta ttggtggcgc
     5461 tgctgctggc tgaagatgcg acgcgttggt tacgtaaagc ttggtctgtc gtccggcgca
     5521 caaaaactaa ggaagaaact tttttgcctt cgcggcgcaa atttatcgcg cagatcggat
     5581 ttggtctggc cgcgatacct tttggttcga ttctctacgg tattacccga ggaaaatatg
     5641 attttaccgt gcaccgcacc gatgttttta tcaaagatct tccttcggtt tttgacggtt
     5701 ttacgatcac ccagctttcc gatattcacg ccggcagttt tgacgatgcc gatgccgtca
     5761 aacgcggcgt tgatctggcc aatgcgcaac gctcggactt gctggtattt accggtgaca
     5821 tggtcaataa ccgtgcggaa gaacttacgc cgtggctgaa tatttttaaa acactgaatg
     5881 cggattacgg gaaatacgcc acgctgggca atcacgatta cggagattat gtatcctggc
     5941 cttcgccgga agccaaagcc gaaaaccttg cgtggctcca atccatgcac gccgagatgg
     6001 gatttacgat gctcaataat gcccacacgg taatttccaa aaacgatgca cacctcgctt
     6061 tagtcggcgt agaaaactgg ggtctgccgc cgtggccgca aaaaggcgat ctaaaaaaag
     6121 cgctggaggg tatagaaccg gcggcgccaa agatattact ctcgcacgat cctacgcatt
     6181 ttgaaggaga agttttgcaa cacgcatcgc gcatcgatct gacgttatcc ggtcatacgc
     6241 acggtatgca attcggcgtg gaaattcccg gattccgatg gagtccggtg cagtatcgct
     6301 acccgcgatg ggccggatta tatactgaaa acgggcgtgc attgtatgtc aatcgcggat
     6361 tcggatttat cggcctgccc ggacgcgtag gcatctggcc ggagatcacg gtgatcacgc
     6421 tgcgttgcgg gtaattgtgg gataatgaga tatgatgttt tgcaataccc atatttacta
     6481 gatgtatttg ttccgataaa attaatcccc aactatcttc atttatttca aatttctttc
     6541 tttcaacaat ctttctaaaa cttttgatct gatgtttttc ggatcgttaa ccaacttata
     6601 gatgaagtat acagggatca gagtaaaaaa tccccaggcg ttttttgcaa cgctataaat
     6661 aataattccc gccataaacc cgacaaacaa agcgttatta atggaaaagg atttcatttt
     6721 tttcgcttca accaacaact cttgatctgt tagctgagat agctcttttt cggccgattt
     6781 tgacaattct ttttgtttca tgtttcttaa tatttgatgg tttagtgttc aatgcgtttg
     6841 tttatagata tgcttttaca attaaaaaat ctcaaaacac tacaaaacgc tcaacttttt
     6901 ttggaatttg attttaaatt ataaaataaa ctttcgaact tttaatcaaa aaaccaatct
     6961 cgcagagacg caaagctaaa tacaaattag aattcttagc ggctttgcga gagcatttgg
     7021 tttgtattgc gcgacgtccc gtagtcttag tcacgcacgc attataccga agatcacaac
     7081 gatcacacca cggtactgtg gttttttaga acaggggttc aaatctatat ttaaacccaa
     7141 atcatagctt taatgttttt attcagctca agtttctctt ttttccgaat ttcggacttg
     7201 agcggaagta tgtatgtttt gtgtttggtg tcaaaccata tcgcagtttc ccattccgta
     7261 tttccgattt tcgccgtcgc tttcagtcgt ccccaaccct cttcctgccg cttaaagttt
     7321 tctctaattt cttttgccat tttgatcggc agagaaacaa agtgccaacc gttgggtgaa
     7381 ggatgctgcc aggttttggc tgaaaattcg tatttgattt taccgttcat agtctgattt
     7441 cgattaggtt ataagatata tgctgacgat tttgggtttg gtgatcgcgc gaaggttcaa
     7501 agcacccatc g
//
LOCUS       DAOQNQ010000155         7462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3738_length_7462_cov_5.589172, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000155 DAOQNQ010000000
VERSION     DAOQNQ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7462
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3738_length_7462_cov_5.589172"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="PK754_10040"
     CDS             complement(<1..1104)
                     /locus_tag="PK754_10040"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNB09888.1"
                     /translation="MHTFLSPKPHVVLSKSFSTPFQNAIATARTCYSAKGIVRDEQIT
                     DKHVPLAQSVYQAGHHTVFSHAYFQFELANVSRQFLWTFLHSHPFYNSEQVSQRYVEV
                     KPDTFLIPALEGEALAIYREAIAFQTAAYFKLNEMLRPSAEAAYFKRFPARAKVREKY
                     AKEIQKKCQEISRYVLPVAAFSYLYHTVSGITLLRYYRLCNQMDTPVEQRVVVEQMVQ
                     EMIRLDPMYKAILEEALPIEETPEYLFYQAHPDFYDDAGRKAFAAEFDRSLDGRMSKL
                     VDYKINNEAVVAQSVREVLGLTRERLSDADAIALALDPSKNKLYGESLNLTTMSKITR
                     ALVHAHYTFRKKISHAADSQDQRHRMTPGSRPIL"
     gene            complement(1157..1930)
                     /locus_tag="PK754_10045"
     CDS             complement(1157..1930)
                     /locus_tag="PK754_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006828437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNB09889.1"
                     /translation="MQNINYTNDNGIITIGLRRPKVNALNRELLGELSEACDRIRQDD
                     TARVVILRSELDNFCAGADLKERQGMSESDVKKFVRYIIGGTIHKIGDLELPTIAAVN
                     GSALGGGCELALACDFRIFSEDVKTGLRETALGIIPGAGGTQRLPRLVGYQKALHWIL
                     TARVFTAQEALHDGVAMEVCTLNELHARAQALAEEILANAPIAVRQAKKAVRLGMGTS
                     LQNALEIENECYNDVIPTEDRLEALKAFSEKRKPNWSNR"
     gene            complement(2116..2559)
                     /locus_tag="PK754_10050"
     CDS             complement(2116..2559)
                     /locus_tag="PK754_10050"
                     /inference="COORDINATES: protein motif:HMM:NF001220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNB09890.1"
                     /translation="MNEQEIRKIVEEARRQLGAQASDETVKHVAMEAIRRLDGDKGYV
                     QEHHSTFRYLNRTEGRIIVTAFGKNGPGIISNISQTLTDCRCDILDVSQKIMQEFFTL
                     MMLVDIKNAACPFGTIKENLARTSETLGIRILAQHEDVFKAMHRI"
     gene            complement(2600..3829)
                     /locus_tag="PK754_10055"
     CDS             complement(2600..3829)
                     /locus_tag="PK754_10055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB09891.1"
                     /translation="MKKETSDSSRNTRTVYDPYRLNINIKSGLFAVAILMIFGVVLYT
                     QYVVEKLRGDSRHLVRIYTHLIAHIGEGDPNGEYSFMFDEIIKRIDFPIINTDPTGEP
                     LSWRNIPGVSGDTMTAATREKLTSVMKEMDVLNTPLPILYEQNVLGYIHYDTDTRLIR
                     QLAWLPYIEIGLVGAFILIGYIGFSNIKRSEQRSIWVGMAKETAHQLGTPLSSMLGWI
                     ELLREQSEKRESTMKILQEMEVDLRRLDRVVARFSQIGSASDLRRQPVVSILQETAGY
                     FRRRLPQTKQIVIEENYDGDPAIFINAQLLGWVIENLVKNAVDAIEKPQGVIKLSLSK
                     SGDSVYIDVADDGRGIDPDNFKNVFRPGFSTKKRGWGLGLSLAKRIVEDYHRGKIFVK
                     ESRPGLGTTMRIQLNAD"
     gene            complement(4010..5143)
                     /locus_tag="PK754_10060"
     CDS             complement(4010..5143)
                     /locus_tag="PK754_10060"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNB09892.1"
                     /translation="MLKFAKWLPLCIILCSGASGCTGGETREAEEIAPGLQYIRIIQH
                     AVPNTIHILELDWPSTRWTVKAVKAGDHIYDTEPLTKVAASILGTIAAINGDFFSQEG
                     SPSGALVSDGVWIKNGAEIWSSVGFTQSGRVYFEAIRPSAHFKAPGERVTSVQGVNRP
                     RLDNEIILYNAYYGRSTATNNFGTEVRLSVRKIPEAWDRPIEAIVREVDSAQGDHPLD
                     DSTWILSGHGAANMQLLRRMRPGMVVRIDMESGLPHRDLRQLIGAYPVLVREKKSILD
                     GALLNKEFARNANARTAIGYSEARKKLILVCVDGKQANYSVGMNLTELADLMIALGCE
                     SASNLDGGGSTTMFIRDRLVNRPSDSGLERPISDALVIVSADN"
     gene            complement(5219..>7462)
                     /gene="dnaE"
                     /locus_tag="PK754_10065"
     CDS             complement(5219..>7462)
                     /gene="dnaE"
                     /locus_tag="PK754_10065"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNB09893.1"
                     /translation="DRNRGKAIEYVQQKYGTDHVAQIITFGTMAARGVIRDVARALTI
                     PLSEADRIAKMIPKRLDITLEKAVAEVPEMKALETHEDERMRSLINVSRSLEGLIRQP
                     GVHAAGVVITPKPMRELVPIYKSAKDEISTQFDKDWVEKIGLLKMDFLGLATLTILED
                     AKKLIRQNYNVDVDFDRIPLDDKKTFDLFWRGETTGIFQFESQGMTEYMKQLKPTTID
                     DLIAMNALYRPGPMEFIPQYIARKNGKEPVDCFHSNLEPYLKSTYGVIVYQEQVMQVA
                     QELAGFSLGKADVVRRIMSKKKPDELAKIKPEWVDGAVARGYKKELAEKIFEILVPFS
                     NYAFNKSHSAAYAIVAYQTAYLKAHYPGEFMAAVLTSEMANTDRIAVLIHACRDMGVE
                     VLPPDVNESMAEFYVKANNIRFGLGAVKNVGLGAIESILQAREKSGRFKSVFDLAERA
                     DLRLVNKKVLESLIQAGACDSLTGHRAQMFLAIEKALSYGQKMQQQANSDQVDIFGQA
                     VQSESRVDIAAIGLPEAPRWTHAEMLQKEKELLGFYASGHPLDRYRAEIEAFSTYHFD
                     DEHELREGEPIRLGGMIAEMRQIFDKKGNPMAFVTLETFGGKMESVVFASVMKECQPM
                     LRKDHVVMLTGKVSIRNEAPSIICDGIYPIGETYQRIRSRVQIHIAQDILQNGILDNL
                     TALLQKAQSANPATSCEVVLCLDFPEKNIIAHYKVSRYKITPTPDILQQIQLLIGQGK
                     IVVKALA"
BASE COUNT         1718 a         1929 c         1862 g         1953 t
ORIGIN      
        1 gagaatagga cgtgagcccg gcgtcatacg atgccgctga tcttgcgagt cggcggcatg
       61 gcttattttt ttgcgaaacg tatagtgcgc gtgcaccaac gcccgcgtta ttttggacat
      121 cgtcgtcaag ttgagcgatt cgccgtacaa tttatttttc gaagggtcga gcgccagcgc
      181 gatcgcatcc gcatcggaga gacgctcacg ggtgagaccc aatacttccc gcacgctttg
      241 ggcaacaaca gcttcgttat tgattttgta atccaccagc tttgacatgc ggccatcaag
      301 gctacgatca aactcggcag caaacgcttt gcggccggcg tcatcgtaaa aatccggatg
      361 cgcctgataa aataaatatt ccggggtttc ttcgatgggc aaagcttctt caagaatcgc
      421 tttgtacatc gggtccaaac gtatcatctc ctgcaccatc tgctcgacga cgacgcgctg
      481 ttcgacaggc gtgtccatct gattgcacaa gcgataatac cgcaacagcg tgataccgct
      541 taccgtgtga tacaaatacg aaaacgccgc caccggtaaa acgtatcgcg aaatttcctg
      601 gcattttttt tgaatttcct tcgcatactt ttcgcggact ttggctctgg cgggaaaccg
      661 tttgaaatag gcggcctccg ccgacgggcg cagcatctca ttgagtttaa aatacgcggc
      721 ggtttgaaag gcaatcgctt cacggtaaat cgccagcgcc tcgccctcca gcgcaggtat
      781 caaaaacgta tcgggcttga cctccacata acgctggctg acctgttctg aattataaaa
      841 cgggtggctg tgtaaaaacg tccacaaaaa ctgacgggag acattggcca attcgaattg
      901 gaaatacgca tgcgagaaga ccgtgtgatg gccggcctga tacacgcttt gtgcgagcgg
      961 tacgtgttta tcggtgatct gctcgtcgcg aacaatacct ttggccgaat aacacgtgcg
     1021 cgccgtagcg atcgcatttt ggaacggcgt cgaaaacgat tttgaaagta ctacgtgcgg
     1081 ttttggggaa agaaatgtat gcaatgtatt agaagccctg ttttgattat ttcacttcga
     1141 tgtcgcgccc gttcacttaa cgattgctcc agttcggttt acgtttttcg ctgaatgctt
     1201 ttaacgcttc gaggcgatcc tcggtcggta tcacatcgtt gtagcattcg ttttcgatct
     1261 ccagcgcatt ttgcaggctc gtacccatac ccagacgcac ggcttttttg gcttgtcgta
     1321 cagcgatagg cgcattggcc agtatctcct ctgccaaagc ttgcgcacgc gcatgcagtt
     1381 cgttcaatgt acaaacttcc atcgccacac cgtcgtgcag ggcttcctgc gccgtaaaca
     1441 cgcgtgcggt gagtatccag tgcaaggctt tctgatatcc gacaagtcgc ggcaaacgct
     1501 gcgtaccgcc ggcgccggga ataataccga gcgccgtttc gcgtaaaccg gttttaacat
     1561 cctcggaaaa aattcgaaag tcgcacgcca gagccaactc acatcctccg cccagcgcac
     1621 tgccgttgac ggctgcgatg gtcggtaatt cgaggtcgcc gattttgtga atggtaccgc
     1681 cgataatgta acggacaaat tttttgacat cggattcgct catgccctgc cgttctttca
     1741 aatcggcacc ggcgcaaaaa ttatccaatt ccgaacgtag aatcacgact cgcgccgtgt
     1801 catcctgacg aatacgatca caggcttcgg ataactcccc gagcaattcg cgattgagcg
     1861 cattgacttt ggggcggcgc aatccgatgg taatgatacc gttatcgttg gtgtagttta
     1921 tattttgcat acaagctgat tctcttggtc attcattgtt agaattttat ttttttaaat
     1981 tcgtcgtaag cccatcatct atgttctttt ctttggcttg atccgaagaa aagaatcaaa
     2041 aaaaaatcac ggctgcagag aacccgcctt ttcaaaacga ttcctattcg tatcttgttt
     2101 actttgaaca aaccattaaa tccggtgcat ggctttgaaa acgtcttcat gctgcgccag
     2161 aatacgaata cccaatgttt cgctggtacg tgccagattt tctttgatgg taccaaaggg
     2221 gcatgcggcg tttttgatat ccacgagcat catcagcgta aaaaattcct gcattatttt
     2281 ttggctgaca tccaaaatgt cacaacggca atcggtgagc gtctgcgaaa tattggaaat
     2341 aataccgggg ccgtttttgc cgaatgccgt cacaatgatg cggccttcgg tgcgattgag
     2401 gtaacgaaac gtgctgtgat gttcctgcac gtaccctttg tcaccgtcaa gccggcgaat
     2461 cgcttccatg gcaacgtgtt ttacggtttc atccgatgct tgtgcaccga gctggcggcg
     2521 cgcttcttcg acgatcttgc ggatttcctg ttcgttcatc gtggtttata ctgttatggt
     2581 taaaaaaaat ggtttccggt taatcggcgt tgagctgaat acgcatcgtc gtacccaaac
     2641 ccgggcggct ttctttgaca aatattttgc cgcgatggta atcttcgaca atccgtttgg
     2701 ccaaactcaa gccgaggccc cagccgcgtt ttttggtact gaagccggga cgaaatacat
     2761 ttttgaaatt atcgggatca atgccgcgtc catcatcggc gacgtcaatg tacacagaat
     2821 cccccgattt actcaaggac aatttgataa cgccctgggg tttttcgatc gcgtccacgg
     2881 cgtttttgac taagttttcg ataacccatc cgagcaattg agcgttgata aaaatggccg
     2941 gatcaccgtc gtaattttct tcgatgacga tctgtttggt ctgcgggaga cggcggcgaa
     3001 aatacccggc ggtttcctgc aaaatcgaaa cgaccggctg acgacgtaaa tccgacgccg
     3061 aaccgatctg cgaaaaacgc gccacgacac gatccaatcg ccgcaggtcc acttccattt
     3121 cctgcaaaat cttcatcgtg ctttcgcgtt tttcagattg ctcgcgcaat aattcgatcc
     3181 agcccaacat ggaggacagc ggcgtaccca actgatgcgc tgtttctttg gccataccca
     3241 cccagatgga acgctgctcg ctgcgcttga tattgctaaa tccgatgtat ccgatcagga
     3301 taaacgcacc gacaaggccg atctcaatat atggaagcca cgccagttgg cggatcaacc
     3361 gcgtatccgt atcgtagtga atataaccga gtacgttttg ttcgtacaaa atcggcagcg
     3421 gcgtattcag cacatccatt tctttcatca ccgatgtcag tttttcgcgc gtggccgccg
     3481 tcatcgtatc tccggaaacg ccgggaatat tgcgccagga aagcggttcg cccgtgggat
     3541 cggtattgat gatgggaaaa tcaatgcgtt tgatgatttc atcaaacata aaactgtatt
     3601 ccccgttggg atcaccttcg ccgatatgtg cgataagatg ggtgtaaatg cgtacaaggt
     3661 gacgcgaatc gccgcggagt ttttcgacga catactgcgt atataatacg acgccgaata
     3721 tcatcagtat cgctacggcg aaaaggccgc ttttgatatt gatgttgaga cggtatgggt
     3781 cgtacaccgt gcgtgtattg cgggatgaat cggatgtttc ttttttcaca tcggttggga
     3841 gttaatttta cccgtcagtt tatgtatgaa tccgcgatca tttcggttcg gcaagcctgc
     3901 ggaatacctg ctcgctaagg cgctaaggca caaagtaata tactaaaaaa ttccatttcg
     3961 ctttgcggct ttgcgaggat atgtttttta tcggtcactt cgttgcgcat taattatcgg
     4021 cacttacaat gacgagcgca tcggaaatcg ggcgttctaa tccgctgtcc gaaggacgat
     4081 tgaccaaacg atcgcgaatg aacatggtcg tagaaccgcc gccatcaaga ttggaagccg
     4141 attcgcaacc gagtgcgatc atcaagtcgg ccagttccgt caggttcatg cccacgctgt
     4201 aattagcttg cttgccgtcc acgcatacca aaatcagttt ttttcttgct tcggaatagc
     4261 cgatcgccgt gcgggcattg gcattgcgtg caaattcttt attgagtaat gcgccgtcga
     4321 gaatactttt cttttcccgt acgagtaccg ggtaagcgcc gatcaattgg cgcaagtctc
     4381 tatgtggcag gccgctttcc atatctatac gcacgaccat acccgggcgc atgcgacgca
     4441 gcaactgcat attggccgcg ccatgcccgc tcaaaatcca ggtggaatcg tccagcggat
     4501 gatcgccttg ggccgaatcc acttcgcgta cgatcgcttc gatcggccga tcccatgctt
     4561 cgggaatttt tctgacgctc agccggactt ccgtgccgaa attattggtc gccgtcgaac
     4621 ggccataata cgcattgtac aaaataattt cattgtccag gcggggacgg ttaacacctt
     4681 gcacggaagt tacacgttca cccggggctt tgaaatgcgc actcggacga attgcttcaa
     4741 aatacacgcg accgctttgt gtgaatccga ccgacgacca tatctccgca ccgtttttga
     4801 tccagacgcc gtcgcttacc aacgcgccgg aagggcttcc ttcctgagaa aaaaaatctc
     4861 cattgatcgc ggcgatagtt ccgagtatgg aagccgccac tttggtaagg ggttcggtgt
     4921 catagatatg atcgccggct ttgacggctt tgaccgtcca tcgcgtggaa ggccaatcta
     4981 actcgagaat gtggatcgta ttgggtacgg catgctggat gatgcggatg tattgcaatc
     5041 ccggggcgat ttcttccgct tcgcgggttt ccccgcccgt acaaccgctc gcccccgaac
     5101 aaagtataat acaaagcggc aaccatttgg caaatttcaa catggcgcta acatcgttaa
     5161 tcgggagatt tcttgcaagc ggaatatcat gcgctcggca ggaaaaggcg gcatccgatc
     5221 aagccagcgc tttgactacg atttttccct gaccgatgag taactggatc tgctggagta
     5281 tgtccggcgt cggtgtgatt ttgtaacggg acactttgta gtgggcgatg atgttttttt
     5341 cgggaaaatc cagacagagg acgacttcgc aggacgttgc cggattagcc gattgggctt
     5401 tttgtaaaag cgcggtgagg ttatccaaaa taccgttttg gagaatgtcc tgtgcgatgt
     5461 gtatctgcac gcgggagcgg atgcgctgat aggtttcccc gatgggatat atcccatcgc
     5521 agataatgct cggcgcttcg tttcgtatcg agactttacc ggtcagcatg accacgtgat
     5581 ctttgcgcag catgggctgg cattctttca tcacgctggc aaagaccacc gattccattt
     5641 ttccgccgaa tgtttcgagc gtcacaaacg ccatcggatt gcctttttta tcaaagatct
     5701 gacgcatctc ggcgatcata ccgccgaggc gaataggttc gccttcgcgc agttcgtgtt
     5761 catcatcaaa gtggtaggtg ctaaaggctt cgatttctgc gcggtagcga tccagcggat
     5821 gacccgacgc ataaaatccc agcagttctt tttctttttg aagcatctcg gcatgcgtcc
     5881 agcgcggcgc ttcaggcaaa ccgatcgcgg cgatatccac acgcgattcc gactggacgg
     5941 cctgaccgaa aatatccact tgatcggaat tggcctgctg ttgcattttt tggccgtagc
     6001 tcaacgcttt ttcgatagcc aaaaacatct gtgcgcggtg gcccgtgagc gaatcgcagg
     6061 cacccgcctg aatcagactt tcgagaactt ttttattgac caatcgcaga tcggcgcgct
     6121 ctgccaaatc gaaaacggat ttgaaacggc ccgatttttc acgtgcctgc aggatggatt
     6181 cgatcgcacc gagacccaca tttttgaccg cacccagacc aaaacggata ttattggctt
     6241 tgacataaaa ctccgccatg gattcattga catccggcgg gagcacctcc acgcccatat
     6301 cgcggcacgc atggatgaga acggcgatgc ggtcggtatt ggccatttca ctggtaagta
     6361 ccgccgccat aaattcgccc gggtaatgag ctttgaggta cgccgtctgg tacgctacga
     6421 tcgcgtacgc tgccgaatgg gatttgttga atgcgtaatt ggaaaacgga acgaggattt
     6481 caaatatttt ttcagccaat tcttttttgt aaccgcgggc gacggcgcca tccacccatt
     6541 cgggtttgat cttggccagt tcatccggtt ttttcttgga catgatacgc cgcacgacat
     6601 ccgctttgcc aagcgaaaaa ccggccaact cctgcgcgac ttgcatcacc tgctcctggt
     6661 acacgataac cccgtaggtg gatttgagat acggctcgag attggaatga aaacagtcca
     6721 ccggttcttt gccgtttttc cgggcgatat actgcgggat gaattccatc ggccccggac
     6781 gatacaaagc gttcatcgcg atcagatcgt ctatcgtcgt gggtttgagt tgcttcatgt
     6841 attcggtcat accctgggat tcaaactgaa aaatcccggt ggtttcaccg cgccaaaaca
     6901 gatcgaacgt ctttttgtcg tccagcggta tgcggtcaaa atcaacatcg acattgtaat
     6961 tttggcgaat tagttttttg gcatcttcca aaatcgtgag cgtcgccaac ccgaggaagt
     7021 ccattttgag cagcccgatt ttttccaccc agtctttgtc aaactgggtc gaaatttcgt
     7081 ccttggctga tttgtagatc ggcaccaatt cgcgcatggg tttgggtgtg atcacgacac
     7141 cggccgcatg aacaccgggc tggcgaatca atccttccag cgaccgcgag acattgatca
     7201 gcgaacgcat acgttcgtct tcatgcgttt ccagcgcttt catttcgggt acttcggcga
     7261 ccgctttttc gagcgtgatg tccagccgtt tgggaatcat tttggcaatg cgatcggctt
     7321 cgctgagcgg tatcgtgagc gcccgcgcta cgtcgcggat gacgccgcgt gcggccatcg
     7381 taccaaacgt gatgatctgc gccacatgat cagtgccgta tttttgctgc acgtattcga
     7441 tggctttgcc ccgattgcga tc
//
LOCUS       DAOQNQ010000156         7414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3776_length_7414_cov_5.144041, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000156 DAOQNQ010000000
VERSION     DAOQNQ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7414
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3776_length_7414_cov_5.144041"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PK754_10070"
     CDS             complement(<1..370)
                     /locus_tag="PK754_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09894.1"
                     /translation="MKKMFLLLALCVATASIAQDQPAGKVHGYVFGDYYYKMGGDKAK
                     AQSNTQYSDPALRKKSAFQLRRMYLYYEHDLSSNFHATFLLEGNDKAMEWAEDTSKAQ
                     VPGRHSVFIKLANLEWKDYIK"
     gene            711..1763
                     /locus_tag="PK754_10075"
     CDS             711..1763
                     /locus_tag="PK754_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNB09895.1"
                     /translation="MKHMQQLLASAVFAGMMMLGACGDKKKEIKNETADPSKTTTAVA
                     ASTAVKVDEGIVAYAKTSGVSGNLNSIGSDTMNNLMTFWAEAFKKFYPSVNVQIEGKG
                     SSTAPPALIEGTAQLGPMSRPMKKEEEDKFEKKYGYKPTGIATSYDGLAVYVHKDNPI
                     KGLTLQQVDAIFSKTRKGGAKEDVVTWGQLGLEGEWKDKPISLYGRNSASGTYGYFKD
                     HALFKGDFKDQVKEQPGSASVVQGVGSDKYAIGYSGIGYTTSEVRAIPVAKDDKSEFV
                     GESYDNVKSGKYPLSRYLYVYVAKHPTKDMDPLTKEFLKFILSKEGQEVVVKDGYMPL
                     TADVVKQQLELVEKKK"
     gene            1849..2778
                     /locus_tag="PK754_10080"
     CDS             1849..2778
                     /locus_tag="PK754_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNB09896.1"
                     /translation="MSNEVLMWIGFNVFVLLMLALDLGIFHKKSHSVGMKEALIWSGV
                     WITLAMVFNIGVYHYMGSEAAMQFLTGYVIEKSLSVDNIFVFVLIFSYFAVPAEYQHK
                     VLFWGIIGALIMRAILIAVGAALISEFHWIIYIFGGFLIITGIKMLVQKEMEIHPESN
                     PVVRFFKKFMSVTPSFEGDRFFVKKDAKTWATPLFVVLLIVEFTDLIFAVDSIPAIFA
                     ITTDPFIVYTSNVFAILGLRSLYFALASVIEKFHLLKLGLALVLIFVGVKMTIVDFYK
                     IPVVIALAVVASILSVSVILSLLIPAKSKTEKS"
     gene            2775..4949
                     /locus_tag="PK754_10085"
     CDS             2775..4949
                     /locus_tag="PK754_10085"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNB09897.1"
                     /translation="MNFLNRRHIYDRLASGIIAAGGFALIVIILGMMGFIIKQALPLW
                     SGAELSTASVIELAQDTNDKSVIKLIGQEEQREILFALTDSGAVRFMRTSDQKELQTF
                     HIPLHKDEHIIHAMEAFASHRIACVTDQGRIIEAEIKFSSQFTGDHRSIIPELHILSV
                     RTFSKTKTPVRGISYAVTASDAYVAVAKENTAYYYKSSDTVPSLVVTDAVIRSMAINP
                     LREEIVLGLEDGHMERWDIRETNAPTRIESVAATAQNGIAVTALAYLIGDISLIVGDE
                     EGRVSGWMEVKHTDGSKNFARIRDFDRHESSIQAITASWRNKCFVTADGRGVFKLFHS
                     TSDRLLVEHKLSYAPSQVSYAPKADGLVFLSGEGRVENFALHNPHPESNWQTLFGEVW
                     YESYDAPGYTWQSTGGTDDFEPKFSLIPLIFGTLKATLYAMLFAAPLAILGALYTSQF
                     AHPRIRTIVKPTVEIMAALPSVVIGFIAGLWLAPLLKNVLIEFIAVITLLPLLIILFV
                     LLFDKLPKKWTNPFRHGYEVFLMLPVLAISIWIGYMVGYWIETNMLGGDVQNWLYQTL
                     GIRYDQRNSIIIGIAMAFAVMPIIFTITEDALSSVPKHLTSASLALGATRWQTAIKVV
                     LPAASAAIFSAVMIGFGRAVGETMIVLMATGNTPIMDWSIFNGMRTLSANIAVEIPEA
                     PEGGTLYRVLFLSGLLLFVITFVVNTLAEIVRQRLRKKYASM"
     gene            4962..6587
                     /gene="pstA"
                     /locus_tag="PK754_10090"
     CDS             4962..6587
                     /gene="pstA"
                     /locus_tag="PK754_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNB09898.1"
                     /translation="MKNLFKSGDIFLWFTGAVLGMIMFLVAGLLLIVSLQGVGVFWPG
                     SVAEVELKDGRRFAGEIMQEENIPLANVPDAYKEAVQTRIQIKTGNRDIYGLDFKWLN
                     TYDIASLQYPDDFAIIERREFGNFYGRVASISENGTIIASGAQATETLDQFLRKALDV
                     HDEQLHLEKGEGGRINRAIEDTRLEIKRLKFYKEDTPELLSSLEAEIHRLQEAYQGIQ
                     KQLETLHLKTSGMYVSVITTDGQTRDLPVSKIVRFYQPNTMNTFEKSGFYISKIFEYL
                     TDEPRESNTEGGVFPAIFGTVMMVFLMNLVVVPFGVVAALYLREYAKQGIAVRLIRIA
                     VNNLAGVPSIVFGIFGLGFFVYFCGGTIDQLFYPDRLPTPTWGTGGILWASLTLSLLT
                     VPVVIVATEEALTTVPKGVRDASLALGATKWQTTWRVVIPASTPGILTGVILAMARGA
                     GEVAPLMITGVVKLAPNLPLDGSFPFFHLDRKFMHLGFHIYDVGFQSPNVEAAKPMVF
                     MTALLLITIVLLLNLTAIIVRARLRKKYQGSAF"
     gene            6629..>7414
                     /gene="pstB"
                     /locus_tag="PK754_10095"
     CDS             6629..>7414
                     /gene="pstB"
                     /locus_tag="PK754_10095"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNB09899.1"
                     /translation="MDNSAPGESTPEQVSHRVKIVSRDINMYYGAHHALKNISVDIHA
                     NEVTALIGPSGCGKSTYLRLFNRMNDLIENVRIEGQILMEGQNIYDKDTDVVELRKKV
                     GMIFQKSNPFPKSIYENVAYGPRVNGENNKTKLEGIVERCLRKSALWDEVKDRLHASA
                     YGLSGGQQQRLCIARALAVDPEVLLMDEPCSALDPIATQKIEDLIEELKRDYTIVIVT
                     HNMQQAARVSDKTAFMWMGELIEFNATKKIFTSPEKKMTEDYIT"
BASE COUNT         1954 a         1606 c         1822 g         2032 t
ORIGIN      
        1 ctttaatata atctttccat tctaagttag cgagtttgat aaaaacagaa tggcgcccag
       61 gcacttgagc ttttgatgtg tcttcggccc actccatggc tttatcgttt ccttcaagta
      121 aaaatgtggc gtggaaatta gaacttaaat catgttcata atacagatac atacgacgta
      181 attgaaaagc gctttttttt cgcaatgcag ggtcggagta ctgtgtattg ctttgagctt
      241 ttgctttgtc gccgcccatc ttataataat aatcgccaaa tacataaccg tggactttgc
      301 cggccggttg atcctgcgca atcgaagccg tcgctacgca caaggcgagc agtaaaaaca
      361 tttttttcat ctctttcttt ctcctgatta aatagcagtt ggttgaaaac tgcgggttca
      421 aaatacatgc aaattctcga aaagataatt ccgatacatt acgccgatat gatgcaaata
      481 ttatggattt gttaagctta taatatgaca aaaatcctat ttcagataaa taaatcttaa
      541 ataagtatag actacttttt atataagtat aaataaaaca atatagattt tgtcttttat
      601 ataacaattt gataacaaat gaataatctt tagaaaacaa gtcaactata aattgctgca
      661 ttattatatc gaaacagaaa catgaattta ataaacgatc aggagaaagt atgaaacaca
      721 tgcagcagtt attagcctcc gctgtttttg ccgggatgat gatgctcgga gcgtgcggtg
      781 ataagaaaaa agaaatcaaa aacgaaacag ccgatccctc caaaacaaca acagctgttg
      841 ccgcttcgac agccgtcaaa gtggatgaag gtattgtggc ttatgccaaa acatccggtg
      901 tttcaggcaa tctgaatagt atcggttccg atacgatgaa taatctcatg acgttttggg
      961 cggaagcgtt taagaaattt tatcccagcg tcaatgtgca gatcgaaggc aagggctcca
     1021 gcacagcgcc tccggctttg atcgaaggca cggcccaact tggtccgatg tcgcgcccga
     1081 tgaaaaaaga agaagaagat aagtttgaaa aaaaatacgg ttacaaacct acaggtatcg
     1141 cgacgtcgta tgatggctta gcggtgtatg ttcacaaaga caaccctatc aaaggtctga
     1201 cgctgcaaca agtggacgca attttttcaa aaacgcgtaa aggcggagcc aaagaagatg
     1261 tggtaacctg ggggcaactc ggccttgaag gcgaatggaa agataaaccg atcagtttgt
     1321 atggccgtaa ttcggcttcc ggtacttacg gatattttaa agatcatgcg cttttcaaag
     1381 gcgatttcaa agatcaagtc aaagagcagc cgggttcggc ttcggtcgtg caaggtgtgg
     1441 gaagcgataa atatgcgatc ggttacagcg gcatcggtta tactaccagc gaagtgcgcg
     1501 ccattcccgt ggccaaagat gataaatcgg aatttgtcgg cgaatcctac gacaatgtaa
     1561 aatccggaaa atatccgctc agccgttatc tgtatgtata tgttgccaaa catcccacga
     1621 aggatatgga tccgctgacg aaagaatttt tgaaatttat tcttagcaaa gaaggtcaag
     1681 aagttgttgt caaggacggt tatatgccgt tgaccgccga tgtggtcaaa caacaactgg
     1741 aattagttga aaagaaaaaa taatcgcacc taattttccc gacggaagtc tgcccgcatg
     1801 tcagatttcc gtcggtatat tacaacctaa ccgaaagctc gaactacaat gtcaaacgaa
     1861 gtgttgatgt ggatcggttt caatgtgttt gttctgctca tgttggcgct tgatctcggg
     1921 atatttcata aaaaatccca ttccgtcggc atgaaagaag cgctgatttg gagcggtgtg
     1981 tggatcacgc tggcgatggt ttttaatatc ggcgtgtatc actacatggg ttctgaagcc
     2041 gcgatgcagt ttttgacggg ttacgtcatc gaaaaatcgc tgagcgtgga taatattttc
     2101 gtttttgtac tgattttttc atacttcgcg gtgcccgccg aatatcaaca taaagtgctg
     2161 ttctggggca tcatcggtgc gcttattatg cgcgccattc tgatcgctgt cggcgcggcc
     2221 ttgatctccg aattccattg gatcatatac attttcggag gttttctgat catcacaggt
     2281 atcaaaatgc ttgtgcagaa agaaatggaa attcacccgg aatccaatcc ggttgtaaga
     2341 ttttttaaaa aattcatgtc cgtaacaccg agttttgaag gcgatcgttt ttttgtaaaa
     2401 aaagatgcca aaacatgggc cacgccgctt tttgtcgtgt tgttgattgt cgaattcacc
     2461 gatttgattt ttgccgtgga ttcgattccc gcgatttttg cgatcaccac cgaccctttc
     2521 atcgtgtaca cgtccaatgt gtttgcgatt ttgggtttac gctcgctgta ttttgccctt
     2581 gccagtgtga ttgaaaaatt tcacttactc aaactcggtt tggcgttagt gcttattttt
     2641 gtcggcgtga aaatgaccat cgttgatttt tacaagatac cggtcgtcat cgccttagcg
     2701 gtggtcgctt ctatactttc ggtttccgta atattgtcac tgctgattcc cgcaaaatct
     2761 aaaacggaaa aatcatgaat tttttgaacc ggcgacatat ttacgatcgc ttagcaagcg
     2821 gcatcattgc cgcgggcgga tttgcgctga tcgtcatcat tttaggtatg atgggtttta
     2881 tcatcaaaca ggcgttgccg ctttggtccg gcgctgaatt gtcaacagcg tcggtgatcg
     2941 aattagccca ggataccaat gataaaagtg taatcaagct gatcggtcag gaggagcaac
     3001 gcgaaattct atttgctctc accgatagcg gtgccgtgcg tttcatgcgt acctctgatc
     3061 aaaaagaact gcaaacgttt cacatcccat tgcataagga tgaacacata atccatgcga
     3121 tggaagcttt tgcatcgcat cgtatcgcct gtgtgacgga tcaaggtcgc atcatcgaag
     3181 cggagataaa gttttcttcg cagttcaccg gtgatcatcg ttccatcatt cccgagcttc
     3241 atattctctc cgtacgaacg ttttcaaaaa ccaaaacgcc ggtccgtggt atttcctacg
     3301 ccgtaacggc ttcggatgcg tatgttgccg tggcgaaaga gaatacggct tattactaca
     3361 aatcatcaga cacggtgccg tcactcgtcg tgaccgatgc ggtgatccgc tcaatggcta
     3421 tcaatccttt gcgcgaagaa atcgtattgg gattggaaga tgggcacatg gaacgttggg
     3481 atatacgcga aaccaatgca ccgacgcgga tcgaatccgt ggcggctacc gctcaaaacg
     3541 gaattgcggt gacagcgctg gcgtatctga tcggcgatat cagtttgatc gtcggagatg
     3601 aagaagggcg cgtctcggga tggatggaag tcaagcatac cgacggcagt aaaaactttg
     3661 cacgcatccg tgattttgat cggcacgaat cttcgataca agccatcacg gcttcctggc
     3721 gcaataaatg ttttgtaacc gccgatggcc gcggtgtatt caaactgttt cattcaacca
     3781 gcgatcggtt gctggtagaa cataagttat cgtacgcgcc gtcgcaggtg agttatgccc
     3841 ccaaggccga cgggttagtt ttcctgagcg gcgaaggcag agttgaaaat tttgcattgc
     3901 ataatccgca tccggaaagc aattggcaaa cgctcttcgg cgaagtatgg tacgagagtt
     3961 atgatgcgcc gggttatacc tggcaatcta ccggcggcac ggatgatttt gaacccaaat
     4021 tcagtctgat accgttgatt tttggtacac tcaaggcaac cttgtatgcg atgctttttg
     4081 cggcgccatt ggctatcctg ggcgcgttgt atacctcgca atttgctcat cctcgcattc
     4141 gtacgatcgt caaaccgacc gtggaaatta tggcggcatt gcccagtgtc gtcatcgggt
     4201 ttatcgcagg tttatggctt gcgccgttgc tcaaaaatgt gctgatcgaa tttatcgccg
     4261 tgataacgct tctgccattg ttgatcatcc tgtttgtatt gttgtttgat aagctgccca
     4321 aaaaatggac gaatccgttt cgtcacggct atgaagtatt tttgatgttg cctgtcttgg
     4381 cgatttccat ttggatcggc tacatggtcg gttattggat tgaaaccaac atgcttggcg
     4441 gtgatgtgca aaactggctt tatcaaacgc tcggtatccg ctacgatcaa cgtaattcga
     4501 tcattatcgg catagcgatg gcgtttgcgg tgatgccgat tatttttacg attaccgaag
     4561 atgcactctc cagcgtacct aaacatttga ccagcgcttc attggcattg ggtgcgacgc
     4621 gttggcagac ggcgatcaaa gtggtgttac ccgcggccag tgcggccatt ttttccgccg
     4681 tgatgatcgg ttttggtcgt gcggtcggtg aaacgatgat cgtactcatg gcgaccggca
     4741 atacccccat tatggactgg agcattttta acgggatgcg tacgctttcc gccaatatcg
     4801 ccgttgaaat cccagaagcg cctgagggtg gaacactgta ccgcgtactc ttcttatccg
     4861 gtttgttgtt atttgtgatc acgtttgtgg tcaatacgct ggcggaaata gtgcgtcagc
     4921 gtttgcgaaa aaaatatgca tccatgtaac ggaataatga cgtgaaaaat ctttttaaaa
     4981 gcggcgatat ttttttgtgg ttcaccggtg ccgtactcgg tatgatcatg tttctcgttg
     5041 cgggcttgct gctcatcgta agcttgcaag gtgtgggcgt tttttggccg ggcagtgtgg
     5101 cggaagtcga actcaaagat ggacgacgtt ttgccggcga aatcatgcag gaggaaaata
     5161 tcccgctggc caatgtgccg gatgcgtaca aagaagcagt gcaaacgcgc attcaaatca
     5221 aaaccggtaa tcgcgatatt tatgggttgg atttcaaatg gctaaacaca tacgatatcg
     5281 cgtcattgca gtatccggat gacttcgcca ttatcgaacg gcgtgaattc ggtaatttct
     5341 acggacgcgt ggcttcgatt tcagaaaacg gaacaatcat cgccagcgga gcacaggcga
     5401 ccgaaacgct ggatcaattt ctccgcaaag ccttggacgt gcatgatgaa caactgcacc
     5461 tcgaaaaagg ggaaggtggt cgcatcaacc gggcgatcga ggatacgcga cttgaaatca
     5521 aacgcctgaa attttataaa gaagatacgc cggaattatt gtcgtcgctt gaggcggaga
     5581 ttcatcgttt gcaagaagcg tatcagggca ttcaaaaaca attggaaacg ttgcatctga
     5641 aaacgagcgg catgtatgtc agcgtgatca ctaccgacgg tcaaacccgc gatttaccgg
     5701 tgtccaaaat cgtgcgtttt tatcaaccca atacgatgaa tacgtttgag aaatccggtt
     5761 tttatataag caaaatattt gagtatctga cggatgaacc gcgcgagtcg aatacggaag
     5821 gcggcgtttt cccggctata ttcggtacgg tgatgatggt gtttttgatg aatctggtcg
     5881 tagttccgtt tggcgtcgtg gccgcgctgt acctgcgcga atacgcgaaa cagggaatcg
     5941 ctgtacgatt gattcgcatc gcggtcaata atcttgcggg cgtaccgtcg atcgttttcg
     6001 gaattttcgg attaggtttt tttgtgtact tctgcggcgg cacgatagat caactttttt
     6061 atccggatcg ccttccgacg ccgacatggg gcaccggcgg tattttgtgg gcatcgctga
     6121 cactttcttt attgacggtg ccggtcgtga ttgttgcaac ggaagaagcc ctgactaccg
     6181 ttcctaaagg cgtacgggat gcgtcgctgg cgttgggtgc gacaaagtgg caaaccacat
     6241 ggcgcgtggt cataccggct tctacaccgg gtattttgac gggtgtgatt ttggctatgg
     6301 cgcgcggtgc aggcgaagtg gcgccgctga tgatcacggg tgtcgttaag ctggctccca
     6361 atctgccgtt ggacggatcg tttcccttct ttcatttgga tcgcaaattc atgcatctcg
     6421 gatttcatat ttacgacgtg ggtttccaat cgcccaatgt agaagcggcc aaacccatgg
     6481 tgtttatgac ggcgttgctt ttgatcacga tagtgctttt gctcaatctg acggcgatca
     6541 tcgtgcgtgc gcgtttacga aaaaaatatc aaggttcggc attttaatgt atattcataa
     6601 agagaacact atggacattc gtatcagtat ggacaattcc gcgccgggtg aatcaactcc
     6661 ggagcaagtt tcacatcgtg tgaaaatcgt ttcgcgcgat atcaatatgt attacggcgc
     6721 acaccatgcg ctcaaaaaca tttcggtgga tattcatgcc aacgaagtca ccgcgttgat
     6781 cggtccgtcg ggttgcggta aatcaacata cctgcgctta tttaaccgga tgaacgatct
     6841 gatcgaaaac gtacgtatcg aaggtcagat tctcatggaa ggacaaaaca tctatgataa
     6901 agatacggac gtggtggagc ttcggaaaaa agtgggcatg atttttcaga agtccaatcc
     6961 gtttcccaaa tccatctatg aaaatgtggc atacggtccg cgcgtcaatg gtgaaaataa
     7021 caaaaccaaa ttagaaggca tcgtcgaacg gtgtttacga aagtcagcct tgtgggacga
     7081 agtgaaagat cgccttcatg catcagcata cggactgtcc ggcggtcagc aacagcgttt
     7141 atgtatagcc cgtgcgctag ccgtggatcc cgaagtgtta ttaatggatg aaccttgttc
     7201 agctttggat ccgatcgcca cacaaaaaat cgaagatctt atcgaagaat tgaagcgcga
     7261 ttataccatc gtcatcgtca cgcacaatat gcaacaagcg gcccgcgtgt cggataaaac
     7321 ggcatttatg tggatgggcg agctgatcga atttaacgcc accaaaaaaa tatttacttc
     7381 ccctgagaaa aaaatgaccg aagattatat cacg
//
LOCUS       DAOQNQ010000157         7366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3805_length_7366_cov_4.344139, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000157 DAOQNQ010000000
VERSION     DAOQNQ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7366
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3805_length_7366_cov_4.344139"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="PK754_10100"
     CDS             complement(<1..1279)
                     /locus_tag="PK754_10100"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNB09900.1"
                     /translation="MKNLIYYLVTAIALTWGNTSYGQGISSDANTLGLWRLDEASGTT
                     ITDASGRGHHGTTGGNPTVVSGRYNNARSFITDSIVVPDHADWSFGSNPFTIECWVNF
                     SSNASHGIIVGQIGSGSDYWRLHYEISANKLEFVYPGSTTQYVWTPVTDQWYHLAVTK
                     SDSVKIYINGIKVASGSIGSPGTVPNLSRPLIFGWGESNYYFKGSIDEIRISNIARSP
                     AEFNLAPAAPANMTATAVSTSQINLSWTASTGGPTKYRIYRSDGIDNNYVQRDSISSP
                     SANSYSATGLGTATTYYFKVAAVNEFGETRSAAAFDTTESAGISFTESSVGSTLFSLG
                     SPLDLGDYDNDGDLDILVAGNNGSTIATKLYSNQNGSFTDELSSIDNLYAGGVHFGDY
                     NNDGFLDFAIMGSTSNYDPPTSGGDPRLKIYKGN"
     gene            complement(1386..2012)
                     /locus_tag="PK754_10105"
     CDS             complement(1386..2012)
                     /locus_tag="PK754_10105"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNB09901.1"
                     /translation="MRQIGWSVVLMLIVFGIGIYGQKQEKERKPTAAVLDFKIISGLT
                     PQEAAALTSKFRNSFSNTGKYTVLERGEMDKILKEQDFKMSDVCNSSECAVEIGQLLA
                     AEKMVTGDIGKIGETYSVTIKLLDVTTGKVDVTINEEYRGKPDGLLTVFDVMAQKITG
                     TYRVSKTKWYILGGVVVAGGAAAAVLLSGGKSGTTASGFPNPPSPPKQ"
     gene            complement(2250..2555)
                     /locus_tag="PK754_10110"
     CDS             complement(2250..2555)
                     /locus_tag="PK754_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09902.1"
                     /translation="MSKNDKYDATREFIDVLTEIIAEPHQHVLDKVILICTRLRGSYV
                     PSEKYVLRQKAVRDVIEMHHRGMPVRLIIEKTGLKKSTVYKIIKDPNGGISEKNNTK"
     gene            2785..3435
                     /locus_tag="PK754_10115"
     CDS             2785..3435
                     /locus_tag="PK754_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB09903.1"
                     /translation="MDSDQLSGTLLKAIDFQLDNADAVNDRFVQWRLIGTANDGEWVQ
                     TWVYCWTRRYFFRKFLCGQIHSAGDTEKLIEEVYFLFIQKHAHVHTPSHFAAWLSVIC
                     KYRFLNYIRSCHPEHWNEALTEVVGNEADKSIVHDLDVKLFFDRIISGEMRQLPDYVQ
                     TVIHKKIWEDKEYHVIAQEMGYSVETIRAYFARGIKALRNSHTIREAYAESYEPNP"
     gene            3460..4086
                     /locus_tag="PK754_10120"
     CDS             3460..4086
                     /locus_tag="PK754_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09904.1"
                     /translation="MFDSNEKLSRQEIADYAMRGHFPLTPEKEESIRRRIDMDPDAQT
                     ELQVMLERKAGLDRLTPSENHFARLQKKTTPSIKSHFSKTLWLRAAAIFLIIVGSYAG
                     LVTYVNYRFDHGPQGLAQFQKDYEFAIAPAATRGSNEKEPEDIRLFKEGVRLLFESKE
                     TTFGLSPRYDEESISHVIEIFEKVEQITENTDLRNRASLIVEKCKSFN"
     gene            complement(4097..4717)
                     /locus_tag="PK754_10125"
     CDS             complement(4097..4717)
                     /locus_tag="PK754_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09905.1"
                     /translation="MKKIGTLTIIVLMAAWLIYLNWPKGTVQILGVDNSGVGLPPPPC
                     PNCLTVESGARPAIGTEKIIIAIPQNSLFEMSLACDALLLDKPGSNKLLFLRFKQGNK
                     PTLEFRNTTAWPGNHFQGLIDNINSTSSKRDYIVLIQSDHIMRITKGNMVMTDFNTIA
                     KELNMRDSSNYGFYREKHVNNLNSGISDKIEIEIEGSGFVKKMDLL"
     gene            4740..7238
                     /locus_tag="PK754_10130"
     CDS             4740..7238
                     /locus_tag="PK754_10130"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNB09906.1"
                     /translation="MKVLEHNNYIFFLVGFHFIVNSLGFSQSATLSEINKMIDGGDLS
                     HASILLDSLLHQSITKKDSLGIGYGYLRLGLCYQNLNDFEKAIELNSHAIPIFLGLKD
                     TLYYAWSLVTEAHYSLIANRFHFVLRNLENVLNLCNATPKLKSIYYDALFRTGAFYLK
                     IGNFDQSIKTLKKAENYFESTYDRFTLSAVLFNMGAAYKETGDYKLSETCLRRVLTLA
                     AGIKQPNDRAKAAYSMLSDLYLSIGKLDQAIAMEYLAIKELKTEKAEADILFCDLGNL
                     FLRLKDFQKGEDAFLKGLSYLATNWNLHSGLAYIAEIRGNIAQADSFHSRAITLANQH
                     IKYTQGTATSAILKKSIPTFNQAARFYWRIGKPEKAFELLETGRARYLSQLLNLANVK
                     SDKSAFTDSIRNIEKQIGELYRKPESGLSTHQLQIFALEAAREQLLDSIKKYSPQFYA
                     FKKTKIPTLKELQTSLGSEFQLIEYAVMDDSIFALTVNSEKVSGHVMQIRRDSLERMI
                     DRVITAKQSSLHDLQQLYWLLITPMEKNLQTGKPLIIIPDDALFRLPFEMLVKNHDGN
                     YRSAKYLLHDFPVSYAVSASVWLDRLGRHSRAPKNYAGFAAAVFDTLSALPFVKNQVD
                     DAADRFSNSTSWTDAADLRRLFVNRAGKYKIIDMATHAFVSDIEPMRSKIVFNEHGGE
                     MNAFEIYELDLDADLAVLSACGTGLGKLSAGEGFMGFNQAFSYAGVYSLIMSAWDIDD
                     ESTAMIMKSFYEGIASGLTRAEALRRAKLKYINEAYELKQSPYYWAGLMLWGNPEAIP
                     LHRVPYGFIAFLFIPFAIIIVSPFVGRLRKKKKH"
BASE COUNT         2047 a         1659 c         1520 g         2140 t
ORIGIN      
        1 cattgccttt gtatatcttt agcctcggat cgcctcctga tgtgggcgga tcatagttag
       61 aagtagatcc cattatggca aaatccaaaa aaccatcgtt attataatcc ccaaaatgga
      121 cgccgcccgc ataaagattg tcgattgagc tcaattcatc agtgaatgaa ccgttttgat
      181 tactgtaaag ttttgttgca attgtagagc cattattgcc ggcaacaaga atgtccagat
      241 cgccgtcatt atcgtaatca cccaaatcaa gtggacttcc taagctaaac aatgttgatc
      301 ctacagagct ttcagtaaat gaaatacctg cgctttcggt cgtatcgaat gccgcggcgc
      361 ttcgcgtttc accgaattca ttaaccgcgg ccactttgaa ataatacgta gtcgctgttc
      421 caagtcccgt cgcgctatag gaatttgcgg acggactgct gatggagtcc cgttgtacat
      481 agttgttatc aatgccatcc gaccgataga tgcggtactt cgtcgggcca cctgtactgg
      541 ccgtccatga gagattaatc tgcgacgtag aaacggccgt ggccgtcata tttgcagggg
      601 ccgccggcgc gagattaaat tcggcaggcg aacgtgcgat attggaaata cgaatttcgt
      661 ctatggagcc tttgaaataa taatttgatt ccccccaacc aaaaatgagc ggtctggata
      721 agttgggtac agtacccgga gagccaatgg atccggacgc aaccttgatt ccgttgatat
      781 atatttttac agagtcgctt ttggtaacag ccaaatggta ccattgatct gtaacgggag
      841 tccatacata ctgcgtcgta gatccgggat agacaaattc cagtttattc gcactgatct
      901 cataatgcaa tctccagtaa tctgatccgg aaccaatttg cccgacaata attccatgag
      961 acgcattgga ggaaaaattt acccagcatt cgatggtgaa gggatttgag ccgaagctcc
     1021 aatcggcatg atcgggtact actatagaat cagtaataaa acttcgtgca ttattgtatc
     1081 gtcctgatac gacagtgggg ttcccgccgg tcgtaccgtg gtgccctctg ccgctcgcgt
     1141 cggtgatcgt tgtacccgat gcctcatcta accgccaaag ccccagcgta ttcgcatcag
     1201 aactaattcc ctggccataa gacgtattac cccacgtcaa agcaatggcg gttaccaagt
     1261 agtaaatcaa gtttttcata ggctctcgtt tgattttgtt tttctattgc acgaccgctt
     1321 ttcacagata agtttaagag ctaattttta agaaatattt taacctgttc ttaatcacaa
     1381 atgcgttatt gtttgggagg acttggcgga tttgggaatc cacttgctgt ggtaccgctc
     1441 ttgccaccgc ttagtaaaac ggccgccgct gcaccgccgg ctactaccac tccgccaagg
     1501 atataccatt tggttttaga aactctatac gtaccggtga tcttctgcgc cataacatca
     1561 aataccgtaa gcaatccgtc cggtttaccg cggtattcct cattgatcgt cacatccact
     1621 tttcctgttg tcacatctaa aagtttgatc gtgaccgaat aagtctctcc tattttgccg
     1681 atgtctccag ttaccatctt ctctgcggcc aataactggc cgatctccac cgcgcactcg
     1741 ctggagttac acacatcgga catcttaaaa tcctgctctt tgagaatctt gtccatctca
     1801 ccgcgctcaa gcaccgtgta tttgccggta ttggaaaacg agttacgaaa tttactcgtc
     1861 agcgcagcgg cttcttgcgg tgtcaatccc gatatgatct taaagtccaa tactgcggcc
     1921 gtaggctttc tttctttctc ttgtttttga ccgtatatac ctatgccaaa cactatcagc
     1981 atgagtacta ctgaccaccc tatttgtctc atgtcttctc tccgtgtttt ttttctgaaa
     2041 tcgttggtat gttttttata tttgtcttgt cacaatcacg cgatgaaata aagcttccga
     2101 ttctgagggt taacggcatg cattcgcatc acagccacca tacgatgcac agcaacaaca
     2161 atccggaaat cgcggcgagc ataatcataa gaccgaaatc tcttaagaaa ttatctttca
     2221 cagctactat ctccacttcg atgattttat tatttcgtat tattcttttc agaaatgccg
     2281 ccgtttgggt ctttgattat tttgtacacg gttgattttt tcaagcctgt tttttcaata
     2341 atgaggcgta ccggcatacc gcgatgatgc atttcgatca catcacgcac cgctttctga
     2401 cgaagtacat atttttcaga tggtacgtaa gaacctctga ggcgcgtaca tattaggata
     2461 accttgtcca atacatgctg gtgcggttcg gcgataattt ctgtaagtac gtctatgaat
     2521 tcacgtgtcg catcgtattt gtcgtttttt gacatagccg taaagccccc ttaaaaaacg
     2581 aatatgagaa ccaaaaataa ggaattagac ccgaaaggcg tgtgctttaa atttagacga
     2641 cagaatttcc atctcccata gcacgcgtgg aaattctgaa tacacgctta gcaaaaaaat
     2701 tcgcttgtac atcgcatttc ctatgtttaa ctttgtatac tcatatagac accgttcttg
     2761 gttatttcat aactacattc cctcatggat tcagaccagc tgtcgggcac attgctcaaa
     2821 gccattgatt ttcaattaga taacgctgat gcggtgaacg atcgttttgt gcaatggcgt
     2881 ctgataggta cggcaaatga cggcgaatgg gtgcagactt gggtttattg ctggacgaga
     2941 agatattttt ttcgcaagtt tctttgcggg caaattcact ctgccggtga taccgaaaaa
     3001 ctgatcgaag aagtgtattt tttattcatt caaaaacacg ctcatgtgca tactccgtcg
     3061 cattttgcgg catggctttc ggtcatctgc aagtatcgct ttctcaacta tattcgttct
     3121 tgccatccgg aacactggaa cgaagcgctg acagaagtgg tcggcaacga agccgataag
     3181 tctatagtac atgatttgga cgttaaactg ttttttgaca ggatcatctc cggcgaaatg
     3241 cgccaacttc cggactatgt acagacggtt attcacaaaa aaatatggga agataaagaa
     3301 tatcatgtga ttgctcagga gatgggatac agtgtggaaa ccatacgcgc gtattttgcg
     3361 cgcggcatca aagcgcttcg caattcccat acaatccgcg aagcctatgc agagagttat
     3421 gaaccgaacc cctaaattgt ctatacagta agagattgta tgtttgactc taacgaaaaa
     3481 ctcagccgcc aggaaattgc cgactatgcg atgcgcggtc attttccttt gacaccggaa
     3541 aaagaagaat ctattcgccg acgcatagat atggatccgg acgctcagac tgaactacag
     3601 gtcatgttgg agcgtaaagc gggattagat cgcctaaccc cctcggaaaa tcattttgca
     3661 cggctccaaa agaaaaccac accgtccata aaatcacatt tctcgaaaac tctatggtta
     3721 cgcgctgcgg ccattttttt gatcatagtc gggagttatg ccggcttagt gacttatgta
     3781 aattatcgtt tcgatcacgg gccgcaaggc ttggctcagt ttcaaaagga ttatgagttt
     3841 gccatagcgc ccgccgcgac ccgcggttcc aatgaaaaag agccggagga tatccggctc
     3901 tttaaagaag gtgtgcgatt gttatttgaa tccaaagaaa cgaccttcgg tttgtctccg
     3961 aggtatgatg aagaatcaat ttctcatgtc atcgagattt tcgaaaaggt cgaacaaata
     4021 accgaaaaca cagacttgcg taatagagcg tcattaattg ttgagaaatg taagagcttc
     4081 aattagatca ttttttttaa agaagatcca ttttcttaac aaaaccggaa ccttcaattt
     4141 caatttcaat tttatcgcta ataccactat ttaaattatt aacatgtttt tcccgataaa
     4201 atccgtaatt agatgagtcc ctcatattta actcttttgc aatcgtattg aagtcggtca
     4261 tgaccatatt tccttttgtg attctcataa tatgatcgga ttgtattaaa actatgtagt
     4321 cacgtttgga acttgttgaa ttaatattat caatcaaacc ttgaaaatga ttgccaggcc
     4381 atgccgtcgt attccgaaat tcaagagttg gtttgttacc ttgtttaaat cgcaaaaaca
     4441 acagcttgtt gcttcctggt ttatctaaaa gtaaagcatc gcaggctaac gacatttcaa
     4501 aaagactgtt ttgtggaata gcaataatta ttttttcagt cccaatcgcg ggtctggcgc
     4561 ccgattctac agttagacag tttggacaag gaggaggggg taatccaacc ccgctattgt
     4621 caacgcccaa aatttgaact gtccccttcg gccaattgag ataaattagc catgcggcca
     4681 taagaacgat aattgttaat gttccgattt ttttcataaa tctctccgaa gttagtttaa
     4741 tgaaagtatt agaacacaat aactatatat tttttcttgt cggttttcac tttattgtaa
     4801 atagtcttgg tttttctcaa tcggctaccc tatcggaaat caataaaatg attgacgggg
     4861 gcgacctttc acacgcatca atattattag acagtctgct gcaccaatcc ataactaaaa
     4921 aagattcctt agggataggt tatggatatc tacgtcttgg gttatgttat caaaacctaa
     4981 atgactttga aaaagcaatt gaattaaact cacatgccat acccattttc ctgggcctta
     5041 aggatacgct gtactatgct tggtcgcttg ttacagaagc gcattatagc ttgattgcaa
     5101 atcgatttca cttcgtttta agaaatctag aaaatgtttt gaatctgtgc aacgctacac
     5161 ccaaactgaa atcaatttat tacgatgcgt tgtttagaac cggtgcgttt tatttgaaaa
     5221 tcggcaattt tgaccaaagc ataaaaactt tgaaaaaagc tgaaaattat tttgaatcta
     5281 cctatgaccg ttttactctc tcggcggttc tctttaatat gggagcggct tacaaagaaa
     5341 caggtgatta taaactttct gaaacctgtt taagaagagt tttaaccctt gctgccggca
     5401 taaagcaacc caatgatcga gcaaaggccg cttattcaat gctatcagat ctttatctta
     5461 gtataggcaa attagaccag gcaatcgcta tggagtatct tgctattaag gaactcaaaa
     5521 cagaaaaagc tgaagccgat atactctttt gcgatcttgg aaatcttttt ctacgattga
     5581 aggatttcca aaagggagag gatgcttttc taaaaggctt gagttatctc gcaaccaatt
     5641 ggaatctaca tagtgggctt gcttacattg ccgaaattcg tggtaatatt gcccaagctg
     5701 attcttttca tagcagggca attacattgg caaaccaaca tataaaatat acacaaggca
     5761 cggcaacaag cgcaatactt aaaaaaagta ttccaacctt taatcaagcc gcccgcttct
     5821 attggcgtat cggaaagccg gagaaggcct tcgagttact cgaaaccggc cgcgctcggt
     5881 acttatccca acttttgaat ttagctaatg tcaaatccga taaatctgct tttaccgaca
     5941 gtataagaaa tatcgaaaag caaatcgggg aactctaccg gaaaccggaa tcgggtttga
     6001 gcacacatca attgcaaatc ttcgccctcg aggccgcgcg ggagcagctt ttggattcta
     6061 taaaaaaata ctctccacag ttctatgcct ttaagaaaac aaaaattccc acactaaagg
     6121 agttgcagac ttcgctgggc tccgaattcc aactcatcga atacgccgta atggacgatt
     6181 cgatttttgc tttgaccgtt aattctgaaa aagtctccgg tcatgtgatg cagatcagaa
     6241 gagattccct ggagcgcatg atcgaccgcg ttatcacggc caaacagtct tcattgcatg
     6301 atttgcaaca attgtattgg ctactcatta cgcctatgga aaaaaatttg cagactggaa
     6361 aaccgcttat catcataccc gacgatgcgc tcttccgcct gccgtttgaa atgctcgtga
     6421 aaaaccacga cggtaattac cgcagcgcaa aatacctttt acatgacttt ccggtgtcgt
     6481 atgcagtttc cgcttccgta tggttggaca ggcttggacg tcattcccgc gcgccaaaaa
     6541 actatgccgg gttcgcggct gctgtatttg acacgttatc agccctgccg tttgttaaaa
     6601 atcaggtcga cgatgcggcg gacaggtttt ctaattccac ttcatggact gacgctgccg
     6661 atttgcggcg actttttgtg aatcgcgcag gaaaatataa gatcattgat atggcaacgc
     6721 acgctttcgt ttccgatatc gaaccgatgc gctccaaaat cgtgttcaac gaacacggcg
     6781 gggagatgaa tgccttcgag atatacgaac tcgatctcga cgccgacctg gccgtactca
     6841 gcgcttgcgg gacggggctt ggtaaactct ctgccggcga aggatttatg ggcttcaatc
     6901 aggcgttttc ctatgccggc gtctacagtc tgatcatgtc cgcctgggat attgatgacg
     6961 aatcaacagc catgattatg aaatcatttt acgaaggcat tgcatccggg cttacccgcg
     7021 cggaagcgct acggcgagcg aaactaaaat atatcaacga ggcgtatgaa ctgaaacaat
     7081 cgccttatta ctgggcgggg ctcatgcttt ggggcaaccc tgaagcaatt cccttgcacc
     7141 gtgtgccgta tggatttatc gcttttttgt tcattccttt cgcgattatt atcgtatccc
     7201 ctttcgtcgg aagattacga aaaaagaaaa aacactgaca aacaatttcg aaatcatctg
     7261 atagctcaat gtttaataat ttcgcttgtt tgtatctgta tcctaaagct catggccata
     7321 ttcaacaatt caaatcctat tttcatgtga atgacatccc tggtat
//
LOCUS       DAOQNQ010000158         7362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3809_length_7362_cov_5.537567, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000158 DAOQNQ010000000
VERSION     DAOQNQ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7362
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3809_length_7362_cov_5.537567"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1388
                     /locus_tag="PK754_10135"
     CDS             <1..1388
                     /locus_tag="PK754_10135"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNB09907.1"
                     /translation="KLFEEKYQVYTAKGALEALELLQQHSVAVAIVDQKMPKITGVEL
                     LKRIKEQYPNIVRIVLTAYTDISDLVASINEGEIFRYITKPWDSEALMWDVHAAIEKY
                     EENLANAQRIDDITRLQLEKEKLQKEIILLKSEIEKEYALDNIVSVSPSMNQIRRIIK
                     AASLADETVLITGESGTGKELVARAIHHNSQRQNFPMVAVDCGALSESLLESELFGHV
                     KGAFTGAVADRKGLFEEADGGTIFLDEISNTSLPLQARLLRVLQEREIRRIGDTKSRP
                     VNVRVIAASNRDLVEECKTERFRSDLFYRLNVLPIALPPLRDRRDDIPLLINYFINEY
                     NKKSAKSIVSISREAMDHLSSLEWKGNIRELKNIIHRMMVFAEGSHLEMGDIPDELLK
                     VKRSAVVPVLPEGSTVFGVSALLPIEDMEREYIRFVLHQTGENKAEASKLLGMKRTTL
                     VMRMKKLGLLA"
     gene            1392..1976
                     /locus_tag="PK754_10140"
     CDS             1392..1976
                     /locus_tag="PK754_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015151312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNB09908.1"
                     /translation="MTRFQYILFYKPYGVICQFSSHEKHPSLAEFKFPKNVYPVGRLD
                     TDSEGLLILTDDGDFQHRLIEPRYGHARTYHAQVEGIPDDGALDKMRHGILLDSKPTL
                     PARVTVLEPPPDHPLRNPPIRYRKNIPTTWVELVLTEGRNRQVRRMTAAAGFPTLRLL
                     RVGIMSLSLEGLQPGSWRAMTTEEFRSCVAVVKK"
     gene            2032..2451
                     /locus_tag="PK754_10145"
     CDS             2032..2451
                     /locus_tag="PK754_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09909.1"
                     /translation="MHFSQSELLINILFLLFIIVLAIQSYILYRVRRSLTQASQFIQL
                     MNVFFKEISKTHSKSASLAVNNQDVMRKLRSKTIRSNPSHDVSCQACKYRLSYVSLDR
                     PEMDFTYACQLTRATVVLDDQCEKFEPDAESAQPSNK"
     gene            complement(2517..3383)
                     /locus_tag="PK754_10150"
     CDS             complement(2517..3383)
                     /locus_tag="PK754_10150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HNB09910.1"
                     /translation="MIRPRNVTLRKRKQLLDNLYNTFDASYIASDPIQMIHCFEQPCD
                     QEIMAVLASAMAFGQVAQINKALSSMLTLMNNQPHYYVTHFDPEHELPRWQKWYYRMI
                     RAEDVLRVLHALKIILQKHQTLGKWIESHYRNEDVHLGITWSRCVNELKDTDKKHWQW
                     RHARGVGFTFLLADPSKKSACKRAFLMLRWMVRKDGIDLGLWNLPTSKLLIPVDTHIQ
                     RIAFYIGLTKRTDTSQKTAMEITYALKKLDAIDPVKYDFAICRLGILKMCPRKRRIVL
                     CRACPIYDICLL"
     gene            3656..>7362
                     /locus_tag="PK754_10155"
     CDS             3656..>7362
                     /locus_tag="PK754_10155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB09911.1"
                     /translation="MIKKRILVAWLFFIGTTFLFSQSREFEFKRLTVENGLSQSNVYA
                     IVQDYRGFIWFGTQDGLNRYDGYQFSVFKHDIDDSNSISMNWINTIYEDKHRVLWVGT
                     QGGGLSRMNQTTEHFRNFTADGQAGSLPSNDVYAILEDHIGNFMIATGRGIVYMDREK
                     ETFRPASEIPELKYGQIRALCVERSGTVWAGTQLGGLFVRDRQGQWFRISMQVAGRPQ
                     SDELAVSSITEDVTGQVWIGTDKGLFNWDNGTMSKYTSPKLENKVLTDGPINHVSTDR
                     AGRVWIGTDNFGLFGIFDTTRSEAVRFINDQLNPRSLSENRVYSTLEDDAGNVWIGTG
                     GGVSIWNARANKFRVYRRSPTSQNTLSHNHVWDILEDRMGFLWVGTNDGLNRLDRKTG
                     NWKNWRYNKTHASGLSAERVWTIYETRDGEIWLGMSGGGLSRYDAATDRFIHFLHDQK
                     NPASINNNRVRSIAEDHDGILWVATRGGGLNRLDRKKNVFTSYQYDGPDGKGISSNDT
                     YVLLVQDSVLWIGTFDNGLNSLNLRTMTFTRYTHDPRNPSSISSNKISSLCLDRNGAL
                     WIGTTEGLNRLNHQRNSFERYTPKDGLPNEVILGVLEDNKGFLWLSTNRGLARFDPLA
                     PRGQKFRNYNPNDGLQADEFNGSSFHKSKSGELFFGGVNGFNAFFPEEIKDNTHVPSV
                     VLTAFKIFDKPFRLQGAIAAMDQVTLGQKENAFSFEFAALDFTAPTKNQYAYKMEGFD
                     EDWIYCGTRRYAGYTNLDGGTYTFRVKASNNDGIWNNEGIAVQVTVVPVFYKTVWFRI
                     LASLVIVLVAYSFYRSRLNRVNRIKELLERKVAERTKDLSEMNAKIMETDRLKSEFLA
                     NMSHELRTPLNAIIGFSELLIDEMRHSANADQLQNLSDIHDSGRHLLMLINDILDLSK
                     IEAGKMELFPQQFIMEELLESVHHTMAPLLNRKNQTLSWQLDTNAPAVYADQNKIKQV
                     LINLLSNAIKFSPHDSAIIVTSKALNTDENDMVEIAVHDHGKGIAPENLDLIFEEFRQ
                     IDGSHARAEQGTGLGLALCRRLIEMHGGKISVESEIHHGSTFRFTIPRAVAGSSQEVM
                     SETPVDLTHQSLILMIEDDPQSANLLRRYLEIAGYRVAHVRNGSEALEEARRLKPMAI
                     TLDIMLPGKDGWQILQELKNDADTSDIPVFIVSVAENKELAYSLKAEDYFTKPIDRER
                     LIHRIGQLRMRRITGEIRQILIVDDDPNALS"
BASE COUNT         2002 a         1536 c         1799 g         2025 t
ORIGIN      
        1 cgaaactttt cgaagaaaag tatcaggtgt acacggccaa aggtgcgctg gaggctttgg
       61 agctgctgca gcaacacagt gtggcggtcg ctatcgtaga tcagaaaatg cccaaaatca
      121 ccggcgtcga attgctcaaa cgtataaagg agcaataccc caatatcgta cgtatcgtac
      181 ttacggcgta cacggacatt tccgatttgg tcgcttcgat caatgagggc gaaattttcc
      241 gttatatcac caagccgtgg gatagtgaag cgctcatgtg ggatgtgcat gcggcgatcg
      301 aaaaatacga agaaaatctt gccaatgccc agcgtattga tgatatcacc cggcttcagt
      361 tggaaaaaga aaagcttcag aaggaaatca ttcttctcaa gtccgagatt gaaaaagaat
      421 atgctctgga taatatcgtc agtgtcagtc cgagtatgaa tcagattcgg cgtatcatca
      481 aggctgcatc acttgccgat gagaccgtac ttattaccgg cgaaagtggc acgggcaaag
      541 agcttgttgc gcgggccatt catcataaca gccaacgtca gaattttccc atggtagcgg
      601 tggattgcgg tgcgttgtcc gaatcgctgc tggagagcga actgttcggt cacgtcaaag
      661 gcgcttttac gggcgccgta gcggatcgta aaggactttt tgaagaagcc gacggcggga
      721 cgattttttt ggatgaaata tcaaacacca gtttgcctct gcaagcacgt ctgttgcggg
      781 ttttgcagga gcgggagatc cggcgtatcg gcgataccaa atcacgcccg gtcaatgtgc
      841 gcgttattgc cgccagtaat cgggatctcg tcgaagaatg caaaacagaa cgttttcgca
      901 gtgatttgtt ttaccgtctc aatgtgcttc cgatcgcttt gccgccctta cgtgatcgcc
      961 gcgacgatat tccgttattg atcaattatt ttatcaatga gtacaataaa aaatcggcca
     1021 aatccattgt ttcgatttcc cgcgaggcga tggatcattt gtcgtcttta gaatggaaag
     1081 gcaatatccg cgaattgaaa aatatcattc atcgcatgat ggtgtttgcg gagggcagtc
     1141 atttggagat gggcgacatt cccgacgaat tgctcaaagt caaacggtcg gcggtggtgc
     1201 ctgttttgcc cgaagggagt acggttttcg gtgtgagtgc gctgcttccg atcgaagata
     1261 tggaacggga gtatatacgg tttgtgttgc atcaaacagg cgaaaataaa gccgaagcgt
     1321 ctaaattact cggtatgaag cgtacgacac ttgtgatgcg aatgaaaaaa cttggacttt
     1381 tagcataacc tttgacgcgg tttcaataca tactttttta taaaccgtac ggtgttattt
     1441 gccagttttc atcgcatgaa aaacatcctt ccttagcaga attcaaattt ccgaaaaatg
     1501 tatacccggt cggtcgtttg gacacggaca gcgaggggtt gttgatcctc accgatgacg
     1561 gtgattttca gcatcgcttg atcgaaccgc gttacggcca tgcgcgcacg tatcatgcgc
     1621 aggtggaggg aattcccgat gacggagcgt tggataaaat gcggcacgga attctattgg
     1681 acagtaaacc cacattgccg gcacgggtga cggtattgga accgccaccg gatcatccgc
     1741 ttcgcaatcc gcctatccgc tacagaaaaa atattccgac gacgtgggtt gagttggtac
     1801 tcaccgaagg tcgaaaccgc caagtgcgac ggatgacagc ggcggctggt tttccgacgc
     1861 ttcgcttatt gcgcgtgggc atcatgagct tgtcattgga gggattacaa cccggttcgt
     1921 ggcgagcgat gacgactgaa gaatttcgct catgtgtggc ggttgtaaaa aaataaagcc
     1981 ggattgcttt tacgccccac gtgaattatc ttagcaaaga aagggagttc tatgcatttt
     2041 tcacaatccg aactgcttat caatattctt tttttgcttt ttatcatcgt attggccatc
     2101 cagtcataca tcctgtatcg tgtgcgtcgt tcgcttacgc aagcaagcca attcattcag
     2161 ctcatgaatg tattttttaa agagatttcc aaaacacact ccaaaagtgc atcattggcg
     2221 gtgaacaatc aggatgtgat gcggaagttg cgaagtaaaa ccattcgttc caacccctcg
     2281 cacgacgttt cgtgccaggc gtgtaaatac agattatcgt atgtcagttt ggatcgcccg
     2341 gaaatggatt ttacctacgc ttgccagttg actcgcgcca cggtggtatt ggatgatcaa
     2401 tgtgaaaaat ttgaaccgga tgctgaatcc gcacagccgt cgaataaata aaaatattct
     2461 caaaagtttg tatcgtttca tcctcttcca cggcacattt catatctcat tttttctcac
     2521 agcaaacaga tatcatagat aggacaggct cggcatagaa cgatccgccg tttgcgtggg
     2581 cacattttga gaatgccgag acgacaaatc gcaaagtcgt attttacagg atctatcgcg
     2641 tctaattttt ttaacgcata ggtaatctcc atcgccgttt tttgtgaagt gtctgtacgt
     2701 ttggttaaac cgatataaaa tgcaattcgc tgaatatggg tatctacggg aatgagtaat
     2761 ttcgacgtcg gtagattcca taatcctaaa tcaataccat ccttacgcac catccatcgc
     2821 aacatcaaaa acgcgcgttt acaggcgctt tttttggatg gatcagccaa aagaaatgta
     2881 aaaccaacgc cgcgtgcatg ccgccattgc caatgttttt tatcggtgtc tttcaattca
     2941 tttacacatc ggctccatgt aatgcctaga tgcacatctt cgtttcgata gtgtgactca
     3001 atccattttc cgagcgtttg atgtttttgt aaaatgattt tcaaagcgtg taggacacgc
     3061 aaaacatctt ctgcccggat catacgatag taccattttt gccaacgcgg taattcatgc
     3121 tccggatcga aatgggtcac ataataatgc ggctgattat tcataagtgt cagcatagag
     3181 gataacgcct tgttgatttg cgctacctga ccgaacgcca tggcggatgc caatacagcc
     3241 atgatttcct gatcacacgg ttgttcgaaa cagtgtatca tttgaatcgg atcgctggcg
     3301 atatacgacg catcaaacgt attataaagg ttatccaaaa gctgttttcg tttacgcagc
     3361 gtaacgttgc ggggtcgaat cacagaggtg tcctttgtga aggagatagg ttgtctaatg
     3421 ttgtcttcca atgtataaac gaaaaacatc cataatgcca attggtattt agtattaaaa
     3481 aaaagcctta ttctcaacta tgatttgaaa ggttcgaata ccggtttttt aattttcttg
     3541 cacaggctct tgagattagt taagttttaa acgcttgcct gttgttctca cttcttaatg
     3601 ttcgctacgt ttcgcgcatg gcgatgacat gccgatggtt atgaaagtaa tgactatgat
     3661 caaaaaacga attttggtcg cttggttgtt ttttatcgga actacctttc ttttttcaca
     3721 atcccgtgaa tttgaattta aacgtctaac cgtagaaaac ggcctgtcgc aaagtaatgt
     3781 gtatgccatt gtacaagact atcgcggatt tatttggttt ggaacgcaag atggcctcaa
     3841 ccgttacgac gggtatcagt ttagcgtttt taagcatgat atagatgata gtaattcgat
     3901 atctatgaat tggatcaata cgatttacga agataagcat cgtgtgttat gggtcggtac
     3961 gcaaggcggc gggctttcgc gcatgaacca aaccacagag cattttcgta attttaccgc
     4021 tgatggacag gccgggtcgc taccgtccaa tgacgtttat gcgatactag aagatcatat
     4081 cggcaatttt atgattgcta ccggccgggg catcgtgtac atggatcgtg aaaaggaaac
     4141 ttttcgtcct gcgtctgaaa ttccggaatt aaaatatggg caaataagag cattgtgcgt
     4201 agaacgctcg ggcactgtat gggccggaac acaacttggc ggtttatttg tacgcgatcg
     4261 tcagggccag tggtttcgca tttcgatgca agtcgctggt cgtccgcaaa gtgatgaatt
     4321 ggccgtttcc tcgataaccg aagatgtcac tggtcaagtt tggatcggta cggacaaagg
     4381 attgttcaat tgggacaacg ggacaatgag caaatacact tcaccgaagt tggaaaataa
     4441 agttctgacg gacgggccga taaaccatgt atccacggat cgtgccgggc gcgtttggat
     4501 aggcacagat aacttcggtc ttttcggaat atttgatacg acacgatcgg aagctgtacg
     4561 ttttatcaat gatcagctca atccgcgcag cttaagtgaa aatcgagttt attcgactct
     4621 tgaagatgat gcgggcaatg tttggattgg tacgggcggc ggagtgagta tttggaatgc
     4681 ccgtgcgaat aaatttcgtg tatatcgccg ttcgccgact tctcaaaata cgctgagtca
     4741 caatcacgtt tgggatattt tggaagatcg tatgggtttt ttatgggtag gcactaatga
     4801 cggcctcaat cgtttagaca gaaaaaccgg taactggaaa aactggcgtt ataataaaac
     4861 acatgcatcc ggattaagcg ctgaacgcgt ttggacgatt tatgaaacac gtgacggcga
     4921 aatttggctt ggtatgtccg gcggcggttt gtcgcgttac gatgcggcaa cggatcgttt
     4981 tatacacttt ctccatgatc aaaaaaaccc agcatcgatc aataacaatc gcgtgcgtag
     5041 tatagctgaa gatcatgatg gtattctgtg ggtcgctaca cgtggtggcg gactcaaccg
     5101 attggatcgt aaaaaaaatg ttttcacatc atatcaatac gatggtccgg acggaaaagg
     5161 tatcagttcc aatgatacgt atgttctgct tgtgcaggat agcgttttat ggataggtac
     5221 gtttgacaat gggttgaata gccttaacct gcgaacgatg acgttcacgc gttatacgca
     5281 cgatcctcgt aatccgtctt cgattagttc caataaaatt tcttcgttgt gtttggatcg
     5341 taatggcgca ttgtggatcg gtacaaccga aggattaaac cgtttgaacc atcaaagaaa
     5401 cagttttgaa agatacacac ctaaagacgg tttacccaac gaagtcatat tgggagtttt
     5461 agaagataat aaaggtttcc tttggctttc gacgaatcgc gggcttgcac ggtttgatcc
     5521 gcttgcccca cgcggccaaa aattcagaaa ctataatccg aatgacggat tacaggctga
     5581 cgaatttaac ggttcatcgt ttcataaaag taaaagcggt gaattgtttt ttggtggtgt
     5641 caacggattc aatgcgtttt ttcctgaaga aatcaaagat aatacgcatg tgccgtcggt
     5701 cgtactgacg gcgtttaaaa tttttgataa accgtttcgt cttcaaggtg cgatagccgc
     5761 tatggatcaa gtgaccttgg gtcaaaaaga aaatgcattc tcgttcgaat ttgccgcttt
     5821 agattttacg gcgcctacaa aaaatcaata cgcgtacaaa atggaagggt ttgatgaaga
     5881 ctggatttac tgcggaacac gccggtatgc gggttatact aatctggatg gtggcacata
     5941 cacatttcgt gtcaaagcgt ctaataacga tgggatttgg aataatgaag gtatcgccgt
     6001 gcaggttaca gtggttcctg ttttttataa aacggtatgg tttcgcatcc ttgcatcatt
     6061 ggtcatagtt cttgtggcgt attccttcta ccgttcgcgc ctcaatcgcg taaatcggat
     6121 caaagagttg cttgaaagaa aagtggccga gcgtacaaaa gacctcagcg agatgaatgc
     6181 caaaatcatg gaaaccgatc gcctaaaatc agaatttttg gcaaatatga gtcatgaatt
     6241 gcgaacgccg ttgaatgcta ttattggatt ttcagaattg cttattgacg aaatgcggca
     6301 ttcggcgaat gcggatcaac tccaaaatct cagtgacatt catgacagcg gtcgccactt
     6361 gttgatgcta atcaacgata tacttgattt atccaaaatc gaagccggga aaatggagct
     6421 ttttccacaa cagtttatta tggaggagtt gttggaatcg gttcaccata caatggctcc
     6481 gctattgaat agaaaaaatc aaacgctgtc atggcagcta gataccaatg cgccggcggt
     6541 gtacgccgat caaaacaaga tcaagcaggt tctgattaat ttattgtcca atgcgatcaa
     6601 gttttctcca cacgacagtg cgatcatcgt cacatcaaaa gcgttgaata ccgatgagaa
     6661 cgatatggtg gaaatagccg tacatgatca cggaaaagga attgcgccgg aaaatctgga
     6721 tttgattttt gaagagtttc ggcagattga tggaagccat gcccgtgcgg agcagggcac
     6781 ggggcttggt ttggcactat gcaggcgatt gatcgaaatg catggcggta aaatcagcgt
     6841 cgaaagcgaa atccatcacg gtagcacatt tcgttttacc atcccgcgcg ccgttgcagg
     6901 atcttcgcaa gaagttatgt cggaaacgcc ggtggatctt acacatcagt cgttgatttt
     6961 gatgatcgaa gatgatccgc agtcggccaa tctgctccga cgctatcttg aaatagccgg
     7021 ttatcgcgtt gcgcatgtgc gcaacggctc ggaagctctg gaagaagcgc ggcgactcaa
     7081 gccgatggcg attacactgg acattatgtt gcccggtaaa gacggctggc agatactgca
     7141 ggaattaaag aatgatgccg atacgtcgga tattccggta tttatcgtgt cggtcgcaga
     7201 gaataaagag ttggcctata gtcttaaagc ggaagattat tttaccaagc cgattgatcg
     7261 cgaacgcttg atccatcgta ttggccagtt gcgtatgcgt cgtatcacgg gcgaaattcg
     7321 tcagatactc atcgtggatg acgatccgaa tgcattgagc at
//
LOCUS       DAOQNQ010000159         7342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3825_length_7342_cov_5.403733, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000159 DAOQNQ010000000
VERSION     DAOQNQ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7342
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3825_length_7342_cov_5.403733"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1594)
                     /locus_tag="PK754_10160"
     CDS             complement(<1..1594)
                     /locus_tag="PK754_10160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNB09912.1"
                     /translation="MNLLSVENAGKAFGVKTLFDNITFGVAQGRKIALVAKNGAGKTT
                     LLKMLMGTEPPDSGRIVFRNNLTIGYLAQEPQFDEKATVLETLFASDTPALRVIKHYE
                     ALLDSPGSDAAYQKALADAMEQMEHLQAWDYEYKIKEILSHLQLHHLHQTIQTLSGGQ
                     RKRVALARVLIEEPELLILDEPTNHLDLDMIEWLESYLSRPEQTLFLVTHDRYFLDRI
                     CDEILELERGTLYRHIGNYSYFLEKKEEREFKEGRELNHTRNVLRKELQWIRRQPKAR
                     TTKSKARVDAFYVLQEKAEEEIPDDKLKLAMQMKRIGGKILELRDIEHRFGNNTILNG
                     FSYSFKKGERIGLVGRNGAGKTTFLEIITGRLHADHGTIETGETITFGYYSQKGLLEN
                     EQQRVIDLVRSYAEVIPMADGSTLSASQFLNQFIFPPELQHTPVYKLSGGEKRRLFLL
                     TVLIKNPNFLILDEPTNDLDLITLSVLEEFLLHFQGCLLIVSHDRYFMDRLVDHIFVF
                     EGDGVIRDFIGNYGEYREAERVK"
     gene            complement(1670..3556)
                     /gene="pepF"
                     /locus_tag="PK754_10165"
     CDS             complement(1670..3556)
                     /gene="pepF"
                     /locus_tag="PK754_10165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HNB09913.1"
                     /translation="MHTMEVLFIFTIGLIAGTVAFAQERDRTKIEEKYKWNLTDIYKS
                     DDDWKKSKDQLILDIQKLAPHKGQLGKSAQHLLDGLNTMFDLSKTFTRLYSYASMSSD
                     QDTRDSKYLAMVQEMSQIGSSFSAAASFIEPEILALDRAMVDGFIKQEPKLAIYKFYL
                     NDILRRQAHTGSAAEEKIIADASLMADGAQNIYSIFSNADFPYPEATFSDGKTVKLDQ
                     ASFGFFRASQNREDRKKTFSTFFGKLNEFRRTYGTQLYAEIKKNIFYKNARKYNSSLE
                     SALDANNIPTDVYHNHVKNVNANLNSFHRYLKLRQRILGVDQLHYYDLYAPLLKNVDL
                     KYSVDEAGKHITTSLAPLGKEYVGVIEKALNERWIDMYPTDGKRSGAYSNGSAYDVHP
                     YMLMNYKGKYDDMSTLTHELGHTMQSYLSNKKQPYPTANYPIFVAEVASTFNEALLIE
                     YMLKNIKDDETRLSLLGSYLEGIKGTVFRQTQFAEFELRIHEIAEKGEALTGDRFNEI
                     YMEITRKYYGHDLGVCIVDDDIKIEWAYIPHFYYNFYVYQYATSFTASAALSEKVISG
                     DKTATQAYLNFLSSGGSKYPIELLKDAGVDMLSTQPIELTMKKMNRVMDEMEKILDRM
                     NKKK"
     gene            complement(3636..4709)
                     /locus_tag="PK754_10170"
     CDS             complement(3636..4709)
                     /locus_tag="PK754_10170"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HNB09914.1"
                     /translation="MQLDIIRVDENKKKIKPTDESTLGFGKIFSDHFFHMTHTTKLGW
                     HKAMIEPYRALSLDPAAVVFHYAQEIFEGLKAYRGKDNGVYLFRSKDNFRRMNRSAVR
                     VCMPELDIEFVNDALKKLVLIDQDWIPRSRGTAMYIRPTMIATEPSVSVKSSDEFLFY
                     ILLGPAGNYYPEGFNPIKIYVSKKHIRAAKGGLGEAKTGANYAASLYTAQEAKKKGFT
                     QVLWLDAFEHRYIEEVGAMNIFFRFKDEVVTSPLGGTILPGITRDSIIQLLKHWGMKI
                     SERPITIDEVAEGAQNGNLLEAFGAGTAAVISPVGEFSHNDQPIIVGQNKVGELSKKL
                     YDELLAIQYGEKPEPFGWVERIG"
     gene            5150..6019
                     /locus_tag="PK754_10175"
     CDS             5150..6019
                     /locus_tag="PK754_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09915.1"
                     /translation="MVARSIVVKWKTMKPGILGFASFALCLTFLVVSCEKPETNVGTN
                     NPGDEQVITVSSKVISTDPKISNSGDLPHYSQSLGVYYTKYYNVGNTRESMSALLIHR
                     IGNTFVSYGNSISLGGKVINRSMDSSSSQGSTYFFYGVDSDIPEGLKLATPPQFVVSN
                     SDQINNISKTLTTKVPIAATNIALGTHIDVSNDFTVQFNRVLEKGGVSINASGYIRTF
                     ELTSPADHLTIPKEELQAIIGENATRGVVVWIWDNSVVDTITSTDKNTNAEYSLPVVE
                     FTAVYVDDVHLDK"
     gene            6107..>7342
                     /locus_tag="PK754_10180"
     CDS             6107..>7342
                     /locus_tag="PK754_10180"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNB09916.1"
                     /translation="MNKLKTAIVFFMFVSACQLFAVDSLTYVRITAPEIESMGGWPMA
                     RKWYGTMINHLRAHGAQQIFVDISFLHADALHPESDAFFYSVVSRESDVYFLNADTSA
                     DTFSFLGIHRLPKAHSFGLFSDFFEISADRMIYHPSQRSLTSILAGPSGPERSVVIEY
                     PDTAIVPEISWLSVIHNTIDRPLHRVVVALDYPGTTSYLYRPDGVAVSTSYTQIWAAH
                     CLIQGHYRYRLSTGVFVMMLILVIGTVIALISWRTSVSVYVTLVSGGILIALVGLMGY
                     AINGWFYLWIMPGLLIGSYRLWQQIMRRNIKPPVAVAVPDKDVNESDYALLKYKLNFY
                     ENAAQTLPDDVLQKSDNESGVLFHETSPLREVLVKAAGVASSDIPVIVQGESGTGKER
                     LAQFIHARSARSEFPFIAVN"
BASE COUNT         1890 a         1535 c         1725 g         2192 t
ORIGIN      
        1 cttttacgcg ttcagcttcc cgatactcgc cataatttcc tataaagtcc cgaatcacac
       61 catcgccctc aaagacaaag atatgatcca caagccgatc cataaaataa cggtcgtgcg
      121 atacgatcag caggcagcct tgaaaatgaa gtaaaaattc ttcaagaacg ctgagtgtga
      181 tcaaatccaa atcattggtc ggctcgtcga ggatcagaaa attgggattc tttatcaaaa
      241 cggtcagcaa aaaaagtcgc cttttttcgc cgccgctgag tttataaacc ggcgtatgct
      301 gcagttccgg aggaaaaata aattgattga gaaactgcga tgcggataat gtcgaaccgt
      361 cagccatcgg aattacttca gcataactcc gcaccagatc aatcacgcgt tgctgttcgt
      421 tttccagaag acctttttgc gaataatatc caaatgtgat cgtttcaccc gtttcgatag
      481 tgccgtgatc ggcatgcaga cggccggtaa tgatttccaa aaacgtagtt ttaccggcgc
      541 cattgcgccc cacaagaccg atgcgttccc cttttttgaa tgaatacgaa aatccgttta
      601 aaatcgtgtt attgccaaac cgatgctcga tatcgcgcag ttccaggatt tttcctccga
      661 tacgcttcat ctgcatggcc aatttaagtt tatcgtccgg aatttcttct tccgcttttt
      721 cctgcaatac ataaaatgca tcaacgcgag ctttagattt ggtcgtacgc gctttgggct
      781 gacggcgaat ccactgcagt tccttacgca atacattgcg ggtatgattc agttcgcgac
      841 cttctttaaa ttcgcgttct tctttttttt ctaaaaaata agaatagtta ccgatgtgtc
      901 tgtagagagt tccgcgttcc aattcgagga tttcgtcgca aatgcggtct aaaaaataac
      961 gatcgtgggt gaccagaaac aatgtttgct ccggtcggga taaataagat tccaaccact
     1021 cgatcatatc gagatcaaga tgattggtcg gttcgtccag tatcaataat tcaggttctt
     1081 cgatgagcac ccgcgcaagc gccacgcgtt tgcgttggcc tccggacaat gtttgtatcg
     1141 tttgatgtaa gtgatgaagc tggaggtggg ataggatttc tttgattttg tattcataat
     1201 cccaagcctg aagatgttcc atttgctcca tcgcatcagc cagcgctttt tgataagcgg
     1261 catccgagcc gggcgaatcc agtaaggctt cgtaatgttt gatcacgcgc agggcgggtg
     1321 tatccgaggc aaaaagcgtt tctaataccg tggctttttc gtcaaactgc ggttcttgtg
     1381 ctaggtaacc gatcgtaaga ttattacgaa aaacgatccg tccgctatcc ggtggttcgg
     1441 tacccatgag cattttgaga agcgttgttt tgccggcgcc atttttagct acgagagcga
     1501 tcttacgacc ttgcgccaca ccaaaagtga tattatcgaa tagcgttttt acaccaaacg
     1561 ctttaccggc attttcgacg gacagaagat tcatacaaaa aactcatttc tgagaaaaga
     1621 aaacgcctga gcggcgaggc ccaggcgttt cgtggatgat aggcgttttt tattttttct
     1681 tattcatgcg atcaaggatt ttttccattt catccatgac gcgattcatc ttcttcatcg
     1741 tgagttcgat cggttgtgtg ctgagcatat ccacgccggc atctttgagc agttcgatcg
     1801 gatacttgga accgcccgat gagaggaaat tcaaatacgc ttgcgtcgct gttttatctc
     1861 cggaaatcac tttttcggat aatgcagcgg aagctgtaaa tgatgtggcg tattgataca
     1921 cgtaaaaatt ataataaaaa tgcgggatgt aagcccattc gattttgatg tcgtcgtcta
     1981 cgatacacac acccaagtcg tgtccgtaat atttacgggt gatttccatg tagatttcat
     2041 tgaagcggtc gcctgtcagc gcttcgccct tttccgcgat ttcgtgaatg cgtaattcga
     2101 attcggcgaa ttgcgtttga cgaaatacgg tgcctttaat gccttcgaga tagctgccga
     2161 gtaaggaaag gcgcgtctca tcatccttga tatttttgag catatattcg atgagcaatg
     2221 cctcattgaa tgtcgaagcg acttcggcaa caaaaatcgg ataattggcc gtgggatatg
     2281 gttgtttttt gttggacaaa tagctctgca tcgtgtggcc gagttcgtgc gtcagtgtgc
     2341 tcatgtcgtc gtatttgcct ttataattca tcagcatata cggatgcaca tcgtaggcgg
     2401 aaccgttgga gtacgcgccg gagcgtttgc cgtcggtcgg gtacatatca atccagcgtt
     2461 cgtttaatgc tttttcgatc acgccgacat actctttgcc caaaggcgct agcgacgtcg
     2521 taatatgttt gccggcttcg tccacagaat atttgaggtc cacgtttttg agaagcggcg
     2581 catacagatc gtaataatgc aattggtcga cgccgaggat acgctgacgc agcttcagat
     2641 aacgatggaa cgaattgaga ttggcgttga catttttgac atggttatga tagacgtccg
     2701 tcggaatgtt attggcatcc agagccgatt cgagggatga attatattta cgggcatttt
     2761 tgtagaaaat attctttttg atttccgcat acagttgtgt gccgtaggtg cgacggaatt
     2821 cattgagttt tccgaagaac gtcgaaaagg tttttttgcg gtcttcgcga ttttgtgaag
     2881 cgcgaaaaaa gccgaacgaa gcctgatcca gctttactgt tttgccgtcg gaaaacgtag
     2941 cttcgggata tggaaaatcg gcatttgaaa aaatgctata gatattttgt gcaccatcgg
     3001 ccatcaggct ggcgtcggct atgatttttt cttccgctgc ggaaccggta tgagcttgac
     3061 ggcgaagaat atcgttaaga taaaatttat aaattgccag ttttggttcc tgtttgataa
     3121 aaccatccac catggcgcga tcaagcgcta agatttcagg ctcgataaag gaagcggctg
     3181 cactgaaaga agatccgatt tggctcatct cctgtaccat tgcaagatat ttggaatcgc
     3241 gcgtatcctg atccgacgac atgctggcat aggagtacaa gcgtgtaaac gttttggaaa
     3301 gatcaaacat tgtattgagt ccgtccaaaa gatgttgagc ggatttgccg agctggcctt
     3361 tatgcggtgc gagtttttgg atatccaaaa tcaattgatc tttggacttt ttccagtcgt
     3421 catccgattt gtagatgtcg gtgagattcc atttgtattt ttcttcgatt ttggtgcgat
     3481 cgcgctcttg tgcaaaagcg accgtaccgg caatcaagcc gattgtgaaa ataaaaagaa
     3541 cttccatcgt atgcatgtaa actccttatg taaataaacg tattttatta actaaaaaaa
     3601 ttcccgcttg cgcgggaatg caatcgttcg atgatttatc cgatgcgttc cacccatccg
     3661 aaaggttccg gcttttcgcc gtattggatg gccagcaatt cgtcatatag ttttttggac
     3721 aattcgccga ctttgttttg tccgacgatg atgggttgat cgttgtgaga aaactcgccg
     3781 acgggtgaaa tgacggctgc cgtacccgca ccgaacgctt ccagtaggtt gccgttttgt
     3841 gcgccttcgg ccacttcgtc aatcgtgatc ggccgttcgg aaattttcat gccccagtgt
     3901 ttgagtaatt gtatgatcga gtcgcgggtg atgcccggaa gaatcgtacc gcctaagggg
     3961 gaagtgacga cttcgtcttt gaaacggaag aaaatattca tcgcgccgac ttcttcgata
     4021 taacggtgtt caaaagcgtc cagccacaat acttgcgtaa aacctttttt cttggcttcc
     4081 tgtgcagtat aaagacttgc tgcgtagttg gcgccggttt tagcttcgcc gagtccgcct
     4141 ttggccgcgc gaatatgttt tttactgacg taaattttta tcggattaaa accttccggg
     4201 tagtagttac ctgccggacc aagaagaata taaaacaaga actcatctga acttttgacg
     4261 ctgacggacg gttcggtggc gatcatggtg gggcggatgt acatcgccgt accgcgacta
     4321 cggggaatcc aatcctgatc tatcaatacg agttttttca gcgcgtcgtt gacaaattcg
     4381 atatctaact ccggcataca aacacgcacg gcggaacggt tcattcgacg gaaattatcc
     4441 ttcgaacgga agaggtacac gccgttgtct tttccgcgat aggctttaag gccctcaaaa
     4501 atttcctgcg cgtagtgaaa aaccaccgcc gccggatcca aactgagtgc gcgatacggt
     4561 tcgatcatgg ctttatgcca acccaacttg gttgtgtggg tcatatgaaa aaaatgatcg
     4621 ctgaaaattt ttccgaaacc gagtgtggat tcgtcggtag gtttgatttt tttcttattt
     4681 tcgtcaacgc ggataatgtc taattgcatg tccgggccct ttataagtta ggcggttaaa
     4741 taaaaagcgg cgcaatatgt ccaagattcg cctattttgc aataaatatc gtgtaactta
     4801 acactatcgg atatgatcaa ggtcagattt atgcttttta acggttctta agtaaccttg
     4861 aattcaccta cttttttata tattaatgaa gtacttgatt gaaataggag tttctgtatc
     4921 tttgtgggta atttagcggt tggtatggat aacaataagc tgctaaatgg agagaaaagt
     4981 ttaaaatttt tgtaagtttt ctatacgtta cagttttcga ttgtagtctt tacagttttt
     5041 atcgttgtta aattttggaa aaacagtgtt tagtgctatg gcatgctatt ttcatatgta
     5101 tctggcgaac gatttggatt aactttaaca acattgaaag gaaagtgtca tggtagccag
     5161 atccatcgta gttaaatgga aaacaatgaa gccgggaata cttggcttcg caagttttgc
     5221 tttatgcctc acttttttgg tggtgagctg cgaaaaaccg gaaacaaacg tcggaacgaa
     5281 taatccgggc gatgagcagg tgattacagt atcgtcgaaa gtcatttcga ccgaccctaa
     5341 aatctctaat agtggcgatt tgcctcatta ctctcaatcg ttaggtgttt attatacaaa
     5401 atactataac gtcggtaata cgcgtgaatc tatgagtgcg ttacttatcc atcgtatcgg
     5461 gaatacattt gtaagctacg gaaattcgat ttctttggga ggaaaagtga taaaccgtag
     5521 tatggatagt tcctcaagcc agggttcaac atattttttc tacggagtag attcggatat
     5581 tcctgaagga ctaaaattgg cgacgccacc gcaatttgtg gttagcaatt cggatcaaat
     5641 aaacaacatt tctaaaacgt tgactacgaa agtgccgata gcggcgacca atatcgcgct
     5701 gggtacgcat attgatgtat ctaatgattt tactgttcaa tttaatcgtg tgttggaaaa
     5761 aggcggggtt tcaataaatg cgtcgggtta tattcgtacc tttgaattga ccagccctgc
     5821 ggatcacctt acaatcccga aagaagaatt acaagccata atcggcgaaa atgcaacccg
     5881 aggtgttgtc gtgtggatat gggataattc ggttgtagat acgatcactt cgactgataa
     5941 aaacaccaac gcagaatatt cgctcccggt ggttgagttt acagcagttt atgttgatga
     6001 cgtacatctg gataaataat atatctcctt catatccctt acgggtgggc gtgttatact
     6061 aaacgcccac tcgttttttg agaaagttga ttacggagcc ggagttttga acaagttaaa
     6121 aaccgccatt gtctttttta tgttcgtatc ggcatgccag ttgtttgctg ttgattcgct
     6181 gacgtatgtt cggattacgg cgcctgaaat tgagtctatg ggaggctggc ctatggcaag
     6241 aaaatggtat ggtacgatga ttaatcattt acgtgctcat ggtgcacaac agatatttgt
     6301 tgacatatca tttttgcatg cggatgcact tcacccggaa tccgatgcgt ttttttactc
     6361 cgtcgtcagc cgagaatcag acgtctattt tctcaatgcc gatacaagtg ctgacacatt
     6421 tagttttctt ggaatacacc ggttaccgaa ggctcattct ttcggcttat tttctgattt
     6481 ttttgagatt tccgctgatc gtatgattta tcacccttcg cagcgatcat tgacttcgat
     6541 attggccggt ccgtcagggc cggagcgatc tgttgtgatc gaatatcctg ataccgccat
     6601 agtacctgaa atttcttggc tatcggttat tcataacact atagatcggc cgctgcatcg
     6661 tgttgtggtg gcgttggatt atcccggtac gacttcatat ttgtacaggc cggatggcgt
     6721 tgccgtttct acatcttata ctcagatatg ggcggcgcat tgtctcatcc agggtcacta
     6781 ccgataccga ttaagtacgg gcgtttttgt aatgatgttg attctggtaa tcggtacagt
     6841 tatcgcatta atctcatggc gtacgtctgt atcggtttac gttacgctgg taagtggcgg
     6901 catactgatc gcattagtgg ggttaatggg ttatgcgatc aacggttggt tttatttgtg
     6961 gattatgccg ggtttgttaa tcgggtccta tcgtttatgg cagcaaataa tgcggagaaa
     7021 tataaaaccg cctgtagcag tagcggtacc ggataaagac gtaaacgaat cagattatgc
     7081 gctcctcaag tacaaactga atttctatga aaatgcagca caaaccttgc cggatgatgt
     7141 attgcaaaaa tcagacaacg aatccggtgt cttgttccac gaaacgtcac cactacgtga
     7201 agttttagtg aaagcggccg gcgtggcatc aagcgatata ccggtgatcg tgcagggcga
     7261 atcgggtacc ggtaaggaaa gattggcgca atttattcat gcgcgttcag cccgatcgga
     7321 atttccgttt attgccgtta ac
//
LOCUS       DAOQNQ010000160         7338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3829_length_7338_cov_5.438556, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000160 DAOQNQ010000000
VERSION     DAOQNQ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7338
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3829_length_7338_cov_5.438556"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            302..1366
                     /locus_tag="PK754_10185"
     CDS             302..1366
                     /locus_tag="PK754_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09917.1"
                     /translation="MKLAKPLLALVYVLTVAVFIACSENNESNKHSNKFHKSQFLEKG
                     NLWKESEFESLLRQNPSKSEALNAVGRSLQVPGVGLVFDEEGYLYTPTQFNQLVEEIT
                     TSTGDDTGAVIDQLMDVYYTDLNTYADVTSDPNAVVENTIGIAPLDSSDEFTSPYYVL
                     DMYQEAYLPKESKLNKLKAEASLFMPKGIEKLSGTPNPPPGIVFPADVVIADRKGSAF
                     GHAGVVIIPGFGNSLQGAMMMEAVGNRKHKADEVVSRPSDKYFFRSDLTYMKICYREN
                     ITIQQRSDITKYVVAQDPDPYSIWTSKDTGGKWYCSKLPWRAYKLKVGCNIDEDGGFW
                     VTPFDIAGDASLKGSYYWHN"
     gene            1438..2508
                     /locus_tag="PK754_10190"
     CDS             1438..2508
                     /locus_tag="PK754_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09918.1"
                     /translation="MRIIWLLFSLCYYGCSTDQKDLYLHTGDKLDSEQFYTGNLTSQE
                     YSECATMIIPGGFAIIKPDTIPTLIKTIQVPPIIYSAQMDQNGLVWVAPPYNQEDDNS
                     ERIYIIDPVTERVVKSITLPQELRTPSCLKISGNKVYFRGDRNGFSVGIGAIDRLTYN
                     NIEAIAKFDTTGFASSPGLEIINNNILAFCETNGRQSSKIVLFNPVTKTIITKVIGNY
                     YRYTYDLNYIYLTVQTAENQIILQKIDPSNLNLLTEVLLGGDEIYITSNQNNICVGSF
                     ANKYIKIVEKSNLTILKTIDFSKQPGIYFNRSFGFITQRMLMLNDNSFYDMQNDIIHS
                     KLFPINYPQTMNLTLAEGQTLK"
     gene            2549..3007
                     /locus_tag="PK754_10195"
     CDS             2549..3007
                     /locus_tag="PK754_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09919.1"
                     /translation="MIKIVLVFICFFCSTATISAQVKTPNAVYVEVIGNGGFYSVNYE
                     RLFCNYFGFRVGASYLYLGDVGQYASFPILANFFMGGKHHKFELGVGCTVMNDAPVGE
                     FSNWDTKYFRTITLGYRYQSKENFFRIGFTPLFTGHEIIPLGGISFGRAF"
     gene            3285..3833
                     /locus_tag="PK754_10200"
     CDS             3285..3833
                     /locus_tag="PK754_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09920.1"
                     /translation="MPSQWFVGLDIGGGGVYWNLDDPSEPGSFNGAGGIFNIRTSINY
                     KMNRFSIGLGGKYDKIYVGELHNTINHDIVKKFDRGYIAQTKFFFIVEYDLFKRKNWS
                     IGSAISAGLAHTKAGLDGSLSKNNPYFGISTYLKWNDHHKLTYSIRPYAEIQSIKNHT
                     TSLQQHTVVDFGCAIGLQFVVF"
     gene            3864..4457
                     /locus_tag="PK754_10205"
     CDS             3864..4457
                     /locus_tag="PK754_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09921.1"
                     /translation="MPSLKLQSLSILFLLISCEAKKDKQNDYDIVNGKVIITASMQNY
                     SFGVCAIMSGSIENELSAYNSSDTVKVFYSDIILPPQYEPYILIQDYPPYAGFEILDD
                     STVVRKFSLKIPKSNGPFTNSNRICVTAWCDTSSDARFDWPFEVGRFPYKHFKNGDSV
                     LVIEFIMDENSEYYTLKNDAPVFLKEKNKYDNFIFRF"
     gene            4494..4913
                     /locus_tag="PK754_10210"
     CDS             4494..4913
                     /locus_tag="PK754_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09922.1"
                     /translation="MRIFLITLILLTTGCSTVMRTAYDSSSAQSMSDEDIQSAISTGK
                     ILNVTYVRDSQKSVTLAGRLEKIETGYRFTPRRKGALGAYKRSMDLTRAEIRKIEVIE
                     KEGLCASEAGTFFLIVGLVLIVAASTVVVASPVGPIF"
     gene            complement(5234..5680)
                     /gene="umuD"
                     /locus_tag="PK754_10215"
     CDS             complement(5234..5680)
                     /gene="umuD"
                     /locus_tag="PK754_10215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HNB09923.1"
                     /translation="MKKEKSSSAFEIFPADITTARHTPLANDTVSAGFPSPADDFVDK
                     MLDLNEHLIKNKAATFLVRVRGTSMINAGIHDGDILIVDRSLEASNGKIVIGVVNGEF
                     TVKRIQKRGMDLYLMPENDQFQPIRINEDMDFKIWGVVTYIIHKSP"
     gene            complement(5677..6945)
                     /locus_tag="PK754_10220"
     CDS             complement(5677..6945)
                     /locus_tag="PK754_10220"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB09924.1"
                     /translation="MRNETYTQLMAIQHPWRGIHLLPKPIWVLFWTTLVNRSGMMALP
                     YLVLYLTKELHYTAEDAAAMIVVYGIGAFLTGPVAGKISDRIGAHRIMILSLVATGLV
                     LVTIPLAHEFWAVACLTVLWAVVSEAFRPASLAVVTNLVSVDQRKLAFAVNRLAINIG
                     MSIGPAVGGFIALHSYPMLFYVNGIFSVLASLVMMFSRWPEAHSAHAAAALHREAESE
                     MHPWQNLSFMYFLIPLLLIMLIFFQIDATVALYLVRDMAYPESHFGLLFSVNTVLIIF
                     FEVWINIRMSHWKHRHALSLGALLIAIGFGLMAFSGHIGYIAFTVAVWTFGEMIIFPG
                     SAAYVSDIAPASRRGEYMGYYQMTFSLGLIIGPWLGVLLYEATDAPTLWLTTLAVGIV
                     AAGMLRLVKEPPAVTMKVLSEAKPGGTDNP"
     gene            complement(7001..>7338)
                     /locus_tag="PK754_10225"
     CDS             complement(7001..>7338)
                     /locus_tag="PK754_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09925.1"
                     /translation="TRRYEPLKHPPLFPLGSIDGLAFYKQSLIAHQSGVVGRIKRIYL
                     DNDLRTATHAAVLEAHNPHFDQPTTGEIGGSFYYYIANAQLRSGFRNKKIKPYHELRD
                     VVILKIPLE"
BASE COUNT         2255 a         1531 c         1517 g         2035 t
ORIGIN      
        1 ccaattccaa ttccaattcc aattccaatt ccaattccaa ttccaattcc aatggaagcg
       61 gaatagacaa ctaatttata tttgtatatg aaggaggtga gtccgttctt caaattataa
      121 tgttttaagc aatcgtatgt gaagtcaatt aattttaacc ggaacaaaaa aggaagaaca
      181 atattaccga attcagcgct gggtgcattg aatttttaaa gtaataaggt cttcctgttg
      241 tcaacctacg aaatgcaacg tagattttca acttttgttt ccaaaatgag gaattaaaaa
      301 aatgaaacta gcgaagcctc ttttggcatt ggtttatgtc ttgactgtag ctgttttcat
      361 cgcatgttct gaaaacaatg agtcaaacaa gcattcaaac aaatttcaca aaagtcaatt
      421 tctcgaaaaa ggaaatttat ggaaagaatc agagtttgaa tcattactaa gacaaaatcc
      481 tagtaaatct gaagcactaa acgcagttgg tcgttcattg caggtacccg gtgtagggct
      541 tgtttttgat gaagagggtt atctttacac ccctactcaa tttaatcaac tagttgaaga
      601 aattactact tctacaggtg acgatactgg tgccgttata gatcagttga tggatgttta
      661 ttatactgac cttaatacct atgccgatgt tacatctgat ccgaatgccg tagttgaaaa
      721 cactattgga atcgctcctt tggattcaag tgatgagttt acatctccat attatgtttt
      781 ggatatgtat caagaagcat atctacccaa ggaatctaaa ctgaataaac tgaaagcgga
      841 agcatcgtta ttcatgccaa aaggaattga gaaactgagt ggaaccccca atcctcctcc
      901 agggatagtt tttcctgcgg atgttgttat tgctgacaga aaaggctcag catttggcca
      961 tgccggtgtc gtcataattc ccggattcgg taacagcttg caaggcgcga tgatgatgga
     1021 agctgtgggt aatagaaaac ataaagcaga tgaagtagta tcaaggccat cagacaaata
     1081 tttttttcga agcgatttga cctatatgaa aatctgttac cgagaaaata ttaccattca
     1141 acagcgctcg gatattacaa agtatgttgt ggcacaagat cccgatccat attctatatg
     1201 gacttcaaaa gatactggcg gcaaatggta ttgttcaaaa ctgccttgga gagcttataa
     1261 gttaaaagtt ggatgcaata ttgatgagga cggtggattt tgggtcactc catttgatat
     1321 tgcaggagac gcatcgctaa aaggttctta ttattggcat aattaaagta aattacatgg
     1381 tggaccaagg gataaaacta tcccttggtt tcatctttaa ggagtgaaag aatatctatg
     1441 agaataattt ggctgttatt cagtttgtgt tactacggat gcagtactga tcagaaagat
     1501 ttatacttgc atacgggtga caagcttgat tcagagcagt tttataccgg gaacctgact
     1561 tctcaggaat actcagagtg tgctaccatg atcattccgg gaggatttgc aattattaaa
     1621 ccggacacaa ttccaacctt aataaaaaca atccaagtcc cccctatcat ttacagcgct
     1681 caaatggatc aaaacggact tgtttgggta gctcctccct acaatcaaga agacgacaac
     1741 agcgagcgca tatatattat tgacccggtt accgagagag tggttaaatc aatcacgttg
     1801 ccccaggagc tgcgaacgcc tagctgcctt aaaatttctg gaaataaagt atattttcga
     1861 ggcgatcgca atggctttag cgtaggtata ggagctattg atagattgac atataataat
     1921 attgaggcaa ttgccaaatt tgatacaacg ggatttgcaa gtagccctgg tttggaaata
     1981 ataaataaca acatacttgc attttgtgaa accaatggcc gtcagtctag taaaattgtt
     2041 ttattcaatc ccgtaactaa aacaataatt acaaaagtga ttggaaacta ttatcgttat
     2101 acatacgact taaattatat ttatttgact gttcagaccg ctgaaaatca aataatacta
     2161 cagaaaatag acccctctaa cttaaactta ttgactgaag tgttattggg cggtgatgag
     2221 atttatatta cttcaaatca aaataatatt tgtgtcggtt catttgcaaa caaatatatt
     2281 aaaattgtgg agaagtccaa tctaacgatt ttaaaaacaa tagatttttc aaagcaaccc
     2341 ggcatctatt ttaatagaag ctttggtttt ataacgcaac gtatgcttat gctcaatgat
     2401 aattcattct atgatatgca aaacgatata atacatagta aactgtttcc tatcaactat
     2461 cctcagacta tgaatttaac cttagccgag ggtcaaactt taaaataaaa gatcattcaa
     2521 agctgagatc aaaattcgga cttaatctat gatcaaaatc gttctggttt tcatttgttt
     2581 tttctgttct actgcgacaa tatcagccca ggtcaaaacg ccaaacgccg tctatgtaga
     2641 agttataggc aatggcggat tttattcggt taattacgag cggttatttt gtaattattt
     2701 tggattcagg gttggcgctt catatttata cttaggtgat gtcggccaat acgcatcatt
     2761 tcctattttg gcaaattttt ttatgggtgg caaacatcac aaatttgaat tgggggttgg
     2821 atgtaccgtg atgaacgacg cgccggtggg agaattttcc aattgggata cgaaatattt
     2881 cagaacaatc actttaggtt atcgttacca gtcgaaagaa aatttttttc ggatcggatt
     2941 tacgccgcta tttaccggtc atgagatcat acctttgggc ggcatcagtt tcggtcgcgc
     3001 tttttgattt atttttttct gattaatgat agttctcgtg cacgtgaatt cagcgataga
     3061 agatagtaga gcaagttttg actatgcgat aacaaggaaa aactatgtag ccatcaaccg
     3121 cttattagtt tggccattcc attaacaaat taaccttaat caaggcgatc aaaatattgg
     3181 tcgccttgtt aatataaatc aaagctttca aaatgaacat catattcaaa actgtattta
     3241 taatcctaat cgcttcaatc cacgtttccg ctcaagatca ttgtttgcca tcacaatggt
     3301 tcgtaggcct tgacataggc ggcggcggcg tttattggaa cttggatgat ccatccgaac
     3361 cgggaagttt taacggagcg ggcggcatat tcaacattcg tacttcgatt aattacaaaa
     3421 tgaatcgttt cagcataggg cttggcggaa agtatgataa aatctatgtt ggcgaacttc
     3481 acaacacgat taatcacgat atagtcaaga aatttgacag aggttacatt gcgcaaacca
     3541 agtttttttt catcgttgag tatgatttat ttaaaagaaa gaattggagc ataggatcgg
     3601 ctatctcagc cggtcttgcg cacactaaag ccggtctcga tggatcgtta agcaaaaaca
     3661 acccttattt cggtatcagc acatatctga aatggaacga tcaccataaa ttaacttata
     3721 gcattcgccc atacgcggaa attcaatcta taaaaaatca taccacttca ttgcaacaac
     3781 atacggtcgt agatttcgga tgcgcgatag gcttgcagtt tgtagttttt taaagggata
     3841 gaaaaaaact ttttatgcga ctcatgcctt cattaaaact tcaatcattg tctatcttat
     3901 tccttctgat ttcctgtgaa gccaaaaagg ataaacaaaa cgattacgat attgttaatg
     3961 gaaaagtaat cataactgca tccatgcaga actactcttt tggggtttgt gctattatgt
     4021 ccggcagtat tgaaaatgaa ctttcagcat ataattcatc cgataccgtt aaagtatttt
     4081 attccgacat tatactgccg ccacagtacg agccatacat tcttatccaa gattatccgc
     4141 cttatgcagg ttttgaaatt ctggatgata gtacagtagt acgaaaattc agtttgaaga
     4201 tacccaagag taatggccca tttacgaata gcaaccgaat atgcgttacg gcgtggtgcg
     4261 acacgtcgag tgacgcgcgc ttcgattggc cgtttgaagt tggacgattt ccatataagc
     4321 atttcaaaaa cggtgatagt gttttggtta tagaatttat catggatgaa aactcagagt
     4381 actatacatt gaaaaacgac gcgccggttt ttttaaaaga aaagaataag tacgataatt
     4441 ttatttttag attttagaaa ttaaagaact atttcatcgc aaggaaaccg tcaatgagaa
     4501 ttttccttat aacattaatt cttctcacca ccggatgcag tacagtgatg cgcacggcgt
     4561 atgattcttc atcggcgcag agtatgagcg atgaagatat tcaatccgcc atatcaacag
     4621 gaaaaatact gaacgtcacg tatgtacggg attcacaaaa aagtgtaact ctggccggaa
     4681 gattggaaaa aattgaaacg ggttaccgct ttacgccccg ccgcaaagga gcgctgggcg
     4741 cctacaagcg gagtatggac ctgacccgcg ccgagatacg taaaatcgaa gtgatcgaaa
     4801 aggaaggcct gtgcgcatca gaagccggta cgttttttct aatcgtagga ctcgtattga
     4861 ttgtagcagc ttccactgtc gtcgtagcat ctcccgtagg ccccattttt taaatcacaa
     4921 gtaagatacc taagggatac tataccttcc gcctacgacg aaattgctcg actaaacatc
     4981 acaaataaaa attatttaga tagaactttg cgcacttagc gtcttcgtgc gtacgcattc
     5041 cctctcgcaa agccgcaatg aaaaaccaag tagatgcgtt gattttacat tgcctcaccc
     5101 gcgcaagcgg gtgtctataa aattatgaga ttctttataa gactcacgaa taaaaattat
     5161 ttagatagaa ctttgcgcac ttagcgtctt cgtgcgtacg cattccctct tgcaaagccg
     5221 caaaaaaacc cagtcaaggc gacttgtgga tgatatacgt tacgacaccc caaattttga
     5281 aatccatatc ttcgttaatg cggatgggtt gaaattgatc gttctccggc atgagatata
     5341 aatccatgcc gcgtttctga atacgcttta cggtgaattc cccgttgact acgccgataa
     5401 cgatttttcc gttggacgct tccagcgaac ggtccacgat cagaatgtcg ccgtcatgaa
     5461 taccggcatt gatcatcgac gtgccgcgca cgcgcacgag aaacgtagcc gctttgtttt
     5521 tgatcaaatg ctcattgaga tccagcatct tatctacgaa gtcatccgcc ggcgaaggaa
     5581 accccgccga aaccgtatcg ttggctagcg gcgtatgacg cgcggtcgtg atgtccgcag
     5641 gaaagatttc aaacgccgaa gatgattttt cttttttcat gggttatccg taccgcccgg
     5701 ttttgcctcc gataagactt tcatggttac cgccggcggc tctttgacca aacgcagcat
     5761 accggccgct acgatgccga cagccaatgt cgtcaaccat aatgtcggcg cgtcggtcgc
     5821 ttcatacaaa agcacaccca accacggccc gatgatcaat ccgagactaa acgtcatttg
     5881 atagtagccc atatattctc cgcgccgcga tgccggtgca atatccgata catacgccgc
     5941 cgagccggga aaaataatca tttccccgaa tgtccacacc gccacggtaa aagcaatgta
     6001 gccgatatgc ccgctgaaag ccatcaatcc gaaacctatc gcaatcagaa gcgctccgag
     6061 actgagcgca tgccggtgtt tccagtgact catacgtata ttgatccaga cttcgaaaaa
     6121 aatgatgagt accgtgttca ccgaaaataa taacccgaaa tgggattccg gatacgccat
     6181 atcgcgaacg agatacagcg ccaccgtcgc atcaatctga aaaaagatca gcatgatcaa
     6241 taatagcgga atcaaaaaat acatgaatga aagattctgc cacgggtgca tttccgattc
     6301 ggcttcacga tgaagcgcgg ccgcggcgtg cgccgaatgc gcttccggcc aacgggaaaa
     6361 catcatcacc agactggcta acaccgaaaa tataccgttc acataaaaca acatcggata
     6421 cgaatggagt gcaataaatc cgccgacggc ggggccgata ctcatgccga tattgatcgc
     6481 aaggcgattg acggcaaaag ctagtttgcg ttggtcaacg gaaaccaaat tggtcacgac
     6541 cgcaaggctc gccggtctga aagcttcact tacgacggcc catagtacgg tgagacaagc
     6601 cacagcccaa aattcatgcg ccaacggaat agtcaccaaa accaaacccg tcgcaaccaa
     6661 agataaaatc atgatgcgat gtgcaccgat gcgatcggaa attttaccgg ctaccggccc
     6721 ggttaaaaac gctccgatac catacacgac gatcatcgcc gcggcgtcct cagcggtata
     6781 gtgcaattct ttggtaagat aaagaacaag gtacggcaat gccatcatgc cgctgcgatt
     6841 gactaaggtc gtccaaaaaa gtacccaaat cggtttcggc agaagatgta tcccgcgcca
     6901 gggatgctga atagccataa gttgtgtata tgtttcgttt cgcaaacata cacagcgtag
     6961 catgaatttc aaaggacgat ttttacgaca aacgcgtgat ttactccaaa gggattttca
     7021 gaattacaac gtctcggagt tcgtgataag gtttgatttt tttattccta aacccgctcc
     7081 gtaattgcgc attggcgatg taataataaa atgaaccgcc aatctcgccc gtcgtcggtt
     7141 gatcaaaatg cggattgtgc gcttccaaaa ccgctgcgtg tgtggccgtg cgcagatcgt
     7201 tatccagata aattcgtttg atccgtccaa cgacacccga ctgatgcgcg atgagcgatt
     7261 gtttgtaaaa agccagcccg tcaatgcttc caagcggaaa tagcggcgga tgtttcaaag
     7321 gctcataccg gcgtgtag
//
LOCUS       DAOQNQ010000161         7317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3845_length_7317_cov_5.146792, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000161 DAOQNQ010000000
VERSION     DAOQNQ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3845_length_7317_cov_5.146792"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..541
                     /locus_tag="PK754_10230"
     CDS             260..541
                     /locus_tag="PK754_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09926.1"
                     /translation="MAVFFLAVSGRPCSEGVGNTDNRMTIRVQDENHEESCADRCSPM
                     DGCACCSHYCICFYIKKMPLHFYFTTYRPASTDVPISLYNSRIFQPPRG"
     gene            577..2757
                     /locus_tag="PK754_10235"
     CDS             577..2757
                     /locus_tag="PK754_10235"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB09927.1"
                     /translation="MKKWILLCMTGIGLSVPQLLNAQHALTVQVTDSESGDAVPGAQV
                     HIMGTTQGGVSNAEGIVRLENIAEGIYRLRITSAGYEDVIMEIQLPRTDANPIPIVLE
                     HASHEGEEITVTSTRSSRTIENTPTRVEAIAAEELDEKANMRVSDIRMQLNESTGIQV
                     QQTSVTSGNAVFRIQGLDGRYTQLLKDGFPLYAGFSGGLSITQIPPLDLQQIEIIKGS
                     ASTLFGGGAIAGLINLVSKTPQEKPETLLMVNGTTAKGFDGNIYHARRNEKTGYSVFS
                     SAHTQSPYDADEDGFSDIPKIHRFALNPRVYYYPGERSTLMMGFDGVLEKRSGGDMQR
                     LENGKDSMHTYIEKNRSRRLSTQMMWQQPLFGRATMTFKNSFSFFDRAIVLQDYRFTG
                     YQQSSFSEWTLGYTHKNNSWIMGINLWTDRFDEQKPSLKRDFHSLTAGVFVQHQAEWS
                     EDWSSEAGLRYDRDKDYGSFVLPRLSVMRRWNASWTSRLGGGLGYKSPTIFNETSETQ
                     SYRNIRGIQKNQLKAETSLGGNFDINYQNVWAKRFPVALNTMVYYTRLRHALTYDKDS
                     LASGVYDLINSDGHIDTRGTEINGKISYEAWKLFVGYTWVDIKRHENGGTSHIPLSAK
                     HRLGLVLVYEEEARWRAGIESYYTGHQYRSDGRSTNDFWIFGFMIQRTMGPYAVFVNF
                     ENFTDTRQSRFETLYTGTRTNPDFKELYAPTEGFVANAGLKIRW"
     gene            2922..3452
                     /locus_tag="PK754_10240"
     CDS             2922..3452
                     /locus_tag="PK754_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09928.1"
                     /translation="MRNIRIVVEYDGTDFHGWQYQSHCRSVQGTLQEAVHRITSENVV
                     VEGAGRTDTGVHARGQVANFKINHAIAEGRIALALNAVLPTDVRVISAQEAPDTFHAR
                     FSARERRYRYRITPIPAALQRHYCWFYPHVMNWEAMQAACAELIGKKFQIVLFVRNRA
                     AALSMRCQVGGMADGK"
     gene            3397..3648
                     /locus_tag="PK754_10245"
     CDS             3397..3648
                     /locus_tag="PK754_10245"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09929.1"
                     /translation="MLPHYQCDVKSAVWLMENDVHVFEIRANRFLHNMVRGIVGTMIM
                     IGRGRLMPGDIATILAKAHRRHAGFNAPPHGLCLEEVIY"
     gene            3798..4388
                     /locus_tag="PK754_10250"
     CDS             3798..4388
                     /locus_tag="PK754_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNB09930.1"
                     /translation="MKIPKLGSDIPTFGNALSRKIGYMILRSMRWRIEGEIPNLKKMV
                     IIGAPHTTNWDFVIGMSALMALGVRVHWIGKDTIFKWPVKYVWHWLGGEPVDRFHAHG
                     VVEQIVQKFSAHDQFILGLSPEGTRRDIPKWRSGFYHVSVGAGVPILLVAFDFPSKTL
                     RIGELFYPTSNPDSDMLQLTGYFKNFRGKFVKTWQK"
     gene            4430..4849
                     /locus_tag="PK754_10255"
     CDS             4430..4849
                     /locus_tag="PK754_10255"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNB09931.1"
                     /translation="MSKHTFTFDPRGKGLRKVLGDLESDIMEVVWEYKSVTVRDVCTH
                     LNKKRPLAYTTVMTVMTRLAEKGVLRKIKSGAAFVYQPARSRESFTLSASVQIFKSLF
                     DDFSKPVMSELIDTIHRENPERLDELAALIEKKKNKK"
     gene            4846..5778
                     /locus_tag="PK754_10260"
     CDS             4846..5778
                     /locus_tag="PK754_10260"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNB09932.1"
                     /translation="MNYFDIIYTEVLTYLGFFVVNPIACAYVAMLQTALLMSAGLIAL
                     GIYKQNRTIKMWKKNSIQPDKRWMATVEKISRRIGLSSLPGLRFTHQALPICTVGFFK
                     PVIYIPEPMVRNLTDSDLAMIAEHELWHIKKRHNLKKLIMRSLGLVGSLWSGFATGLA
                     LTYRVLDQRYEIIGFVLSFILGLILMRAFRRYVIPYFDLWCEYECDDALQMCRNQRQT
                     MAAMLVRLGKMNIVSDPMRDMIRCAAVSHLEKRVRRLISEPASNTFTPYIRGVLYFVA
                     IVLIVNVINIHREHNLQWVSGHGKRMHLCAGQCK"
     gene            5831..6295
                     /locus_tag="PK754_10265"
     CDS             5831..6295
                     /locus_tag="PK754_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09933.1"
                     /translation="MKTLLMIAFLVPCIGCRVNPDLEAEKTELLRLHEAQRRAHFDRN
                     VDPMTEGHVPQRIQISGGELSYTTPEQTRARFERYFGRVEFLRWDDTIEPIIVISDDA
                     TLANTFVQKLVVVSYQDERGAKQLDTTRFAWTTTYRKINDQWQMIAVTSTRK"
     gene            6306..>7317
                     /locus_tag="PK754_10270"
     CDS             6306..>7317
                     /locus_tag="PK754_10270"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNB09934.1"
                     /translation="MVRIGLFCFLFHSAVAQNDIMLLYRDAMALMVKKNFEHAEQKLR
                     ILDSLMPDDTEIYFLRAQVLAQMGDHTKAMGLLKASLIMGSDMLFAIGKDSAFVGLWA
                     DSEFQNLLKEMEQLKRPVHHSRDAYLLTERDLIPEGTAYDGHTGQLFISSTYKGKIKR
                     IYPDGRSDDFTKEGQDGLLGVVGMEVDSVRRILWVNSCNNPNMPIRDSHADARVPARV
                     HKFDLTTGRLLQRYDAPAGSHFFNDLTVTAKGDVFITDTESGEIWQIAAERHTLEIFL
                     KLQNVLFLNGITKSPDDQWLYVAHVAGILVIDIATRRYEPLKHPPLFSLGSIDGLAFY
                     KQS"
BASE COUNT         1971 a         1560 c         1819 g         1967 t
ORIGIN      
        1 aaaaaaaaga aaggaactac ccatgccctg cgaaaaaaaa cgaaaaagaa aaaaaatggc
       61 tactcaaaaa cgaaaaaaac gtcttcgtcg cgaccgccat aagaaaaaag gccgctagtt
      121 ttttgatttt ttagctgatc gtttgtacta taagtcccgt tgcactaacg ggactttttt
      181 tgttactacg atgaacaatt tttttatatc tcgacaaaaa caagtgcacc ggatcataaa
      241 ggaaatcacg gcgctttatt tggccgtctt ttttttagcc gtgagcggtc gcccttgttc
      301 ggaaggagtc ggcaataccg ataaccgcat gacgatccga gtgcaggatg aaaaccacga
      361 agaatcctgc gccgataggt gttcgccgat ggacggttgt gcgtgctgtt cgcattattg
      421 tatttgtttt tatatcaaaa aaatgccgct tcatttttat tttacgacat accggccggc
      481 ttctacggac gtacccattt ccctatataa cagcagaatt tttcaaccgc cgcgtggata
      541 acgtttttaa cgtttattta caccaacgga gatgatatga aaaaatggat tttattatgt
      601 atgacaggga tcggtctgtc cgtaccgcaa ctgctaaatg cacaacatgc gctcacagtg
      661 caagtaaccg attccgaatc gggcgacgcg gtacccggtg cacaagtgca tattatgggt
      721 acaacccaag gcggcgtttc gaatgcggaa ggcattgtac gacttgaaaa tatcgccgaa
      781 ggcatttatc ggctgcgcat cacttccgca ggttatgagg acgtgattat ggagattcag
      841 ttaccgcgca cggacgcgaa cccgatccct attgttctgg aacatgcctc gcatgaaggc
      901 gaagaaatca ccgttacgtc cacgcgttcg agtcgaacga tcgaaaatac accgacgcgc
      961 gtcgaagcca tcgctgcgga ggaattggac gaaaaggcca atatgcgcgt gagtgatatt
     1021 cgcatgcagc tcaatgagag tacaggtatt caggttcagc aaacgtcagt tacctccggt
     1081 aatgcggtgt ttcgtatcca gggtcttgat ggccgttaca cacaattgct caaagacggt
     1141 ttcccgctgt acgccggttt ttccggcggg ctcagcatca cgcaaatacc gccgcttgat
     1201 cttcagcaaa tcgagatcat caaaggcagt gcatctacgc tattcggcgg tggggctatt
     1261 gcagggctga tcaacttggt aagcaaaacg ccgcaggaaa aaccggagac tttactgatg
     1321 gtcaacggta cgacggctaa gggttttgac ggcaatatct atcacgcgcg gcgaaatgaa
     1381 aaaacagggt atagtgtgtt ttcatcagcg catacccaaa gcccgtacga tgccgatgag
     1441 gatgggtttt cagatattcc taaaattcat cgcttcgcgt tgaatccccg ggtttattat
     1501 tatccgggtg aacgaagtac gctgatgatg ggatttgacg gtgttttgga aaaaaggagc
     1561 ggcggcgaca tgcagcgcct cgagaacgga aaagattcga tgcacacgta tatcgaaaaa
     1621 aaccgttcac ggcgcttgtc cacacagatg atgtggcaac aacctctttt tgggcgagcc
     1681 acgatgacat ttaagaacag cttcagtttt tttgatcgtg cgattgtgct gcaggattat
     1741 cgttttaccg gataccaaca atcttctttt tccgaatgga cgttgggata tacgcataaa
     1801 aataattcat ggatcatggg catcaacctg tggacggatc gttttgatga acagaagcca
     1861 tccctaaaac gtgattttca tagcctgaca gcgggggtat ttgttcaaca tcaggcggag
     1921 tggagtgaag attggtcatc cgaagccggt ctgcgatatg atcgcgataa agactacggt
     1981 tcgtttgtac tacctcgttt gtccgtaatg cgccgctgga atgcttcgtg gacatcgcgg
     2041 ctcggcggag gacttggtta taaatcgccg acgatattca atgaaacgtc ggaaacgcag
     2101 tcctatcgca acattcgcgg tattcaaaaa aatcaactta aagccgagac atcgcttggc
     2161 ggaaattttg atatcaatta tcaaaacgtt tgggcaaaac gattccctgt ggcgctcaat
     2221 accatggtgt attatacgcg gttgcggcac gcgctgacgt atgacaaaga ttcgctggcg
     2281 tcgggcgtgt acgatttgat caattccgac gggcatattg atacacgcgg tacggagatc
     2341 aacggaaaaa tcagttacga agcctggaaa ttatttgtcg gttatacatg ggtggatata
     2401 aagcgtcatg aaaacggcgg tacatcgcat ataccgctat cggctaaaca ccggcttggg
     2461 cttgtactcg tgtatgaaga agaggcacgt tggcgtgcgg gtatcgaatc gtattatacc
     2521 ggtcatcagt accgcagtga tggtcgttct acaaatgatt tttggatttt tggttttatg
     2581 attcagcgca ccatgggccc gtatgccgta tttgtcaatt ttgaaaactt cacggatacg
     2641 cgtcaatccc gctttgaaac gctgtacaca ggtacacgaa ccaatccgga ttttaaggaa
     2701 ctctatgcgc cgacggaggg ttttgtggcg aatgcaggtt taaaaatacg ctggtaagca
     2761 tcacgtaccg aatacgggtt tttgattttg tagttgttcg tttgtactat aatcccgccg
     2821 aataaaacgg cgggatattt tttttaaata cttcataaaa cgtcaacgag tatatttcat
     2881 agtatacgtc gtttattttt aatcagtaat acaatcacgg tatgcgcaac atacgtatag
     2941 tcgtagaata cgacggcacg gattttcacg gatggcaata tcaatcgcac tgtcgctccg
     3001 tacaaggtac gctgcaggaa gccgtacatc gcatcacatc cgaaaacgtc gtcgtcgaag
     3061 gtgcggggcg taccgatacg ggtgtgcatg cacgcggaca ggtggccaat tttaaaataa
     3121 atcacgctat cgctgaagga agaatcgcgt tggcactaaa cgccgtcctg ccgacggatg
     3181 tgcgcgtcat ttcggcacag gaagcgccgg atacatttca tgcgcgtttt tcggcgcggg
     3241 aacgtcgtta tcgttatcgt atcacaccga tacctgccgc attacaacgt cattattgtt
     3301 ggttttatcc ccatgtaatg aattgggagg ccatgcaagc tgcgtgtgcg gaattgatcg
     3361 gaaaaaaatt tcaaatcgtt ttgtttgtca gaaaccgtgc tgccgcatta tcaatgcgat
     3421 gtcaagtcgg cggtatggct gatggaaaat gatgtccacg tgtttgaaat acgggcgaat
     3481 cgttttttgc ataatatggt acggggtatc gtcggaacca tgatcatgat cggtcgtggc
     3541 cgtcttatgc cgggtgatat cgcaacgatc ttagctaaag cacatcgccg ccacgcggga
     3601 tttaatgcgc cgccgcacgg tttgtgctta gaggaagtga tttactgacg cggaaaaatc
     3661 atatcattgc cgccacacaa aactacttca tttctatatg acagcgtgtc ctcttcgcag
     3721 gtaaacttca taataatttc ctttgcagct aagtgattta tgcgcgtaca ttgagggcga
     3781 acatactttg ttgtgctatg aaaattccta aactcggcag cgacatacct acatttggta
     3841 acgctctatc ccgtaaaatc ggttatatga ttttacgatc catgcgttgg cgtattgagg
     3901 gcgaaatacc taacctgaaa aagatggtaa ttatcggtgc gccgcatacg acgaattggg
     3961 attttgtaat cgggatgtcg gctttaatgg cgctcggtgt tcgcgtgcac tggatcggaa
     4021 aagatacgat tttcaagtgg cctgtaaaat atgtctggca ctggcttggc ggggaaccgg
     4081 tggaccgatt tcacgcacac ggcgtggtag aacaaatcgt tcaaaagttt agcgcgcacg
     4141 atcagtttat tctcgggctg tcgcccgaag gcacgcgaag agatattcca aaatggcgca
     4201 gcggttttta tcatgtgtcc gtcggtgccg gtgtacccat attattagtc gcatttgatt
     4261 ttccgtcgaa aacactgcgt atcggcgaat tgttttaccc cacgtcaaat cctgattcgg
     4321 atatgctgca gttaaccgga tactttaaga acttccgcgg aaaatttgtt aaaacgtggc
     4381 agaagtaatt ttttttgaaa ctttaaaaac tacataacgt agtaggatta tgtctaaaca
     4441 tacatttaca tttgatccgc gcggtaaagg tttacgcaaa gtgcttggcg atctggagtc
     4501 cgatatcatg gaggtggtat gggaatataa atcggtcacc gtgcgtgatg tatgcacaca
     4561 cctcaacaaa aagcggcctt tggcttatac taccgtgatg acggttatga cgcggttggc
     4621 agaaaaaggc gtattgcgaa aaatcaaatc cggcgccgcg tttgtttacc aaccggcacg
     4681 atcacgggaa tcgttcacgt taagcgcatc ggttcaaatt tttaaaagcc tttttgatga
     4741 tttttcaaaa ccggtaatga gcgaactgat tgatacgatt catcgggaaa accccgaacg
     4801 attggatgag ttggccgcat tgatagagaa aaagaaaaat aagaaatgaa ttacttcgac
     4861 attatctata cggaggtatt gacttatctc gggttttttg ttgtgaatcc gattgcttgc
     4921 gcgtacgtcg caatgttgca aacggcactc ttgatgagcg cgggtcttat tgcgctgggt
     4981 atttataagc aaaaccgtac cataaaaatg tggaaaaaaa attcaataca acccgataaa
     5041 cgatggatgg ccactgttga aaaaatcagt cgccgtatcg ggctctcttc gctaccggga
     5101 ttacgtttta cccatcaggc gcttcctatt tgtacggtgg gtttttttaa accggttatt
     5161 tatataccgg agccgatggt gcgaaattta acagattccg acctcgcgat gattgcagaa
     5221 catgaactgt ggcatataaa aaaacgccac aatctgaaaa aattaatcat gcgttcattg
     5281 gggctcgtgg gttcgctttg gtcgggtttt gcaaccggac tcgcactgac ctatcgtgtt
     5341 ttggatcaac ggtatgaaat catcggattt gtgctttcgt ttatattagg tctaattttg
     5401 atgcgcgcat tccgtcgtta tgttataccg tattttgatt tgtggtgcga gtatgaatgc
     5461 gacgatgctt tgcagatgtg tcgtaatcag cgacaaacta tggcggcaat gctggttcga
     5521 ttgggaaaaa tgaatattgt ttccgatccg atgcgggaca tgatccgatg tgcagcggtt
     5581 tcgcatttgg agaagcgcgt tcgacggtta atcagtgaac ccgcgtcaaa cacgtttacg
     5641 ccttatattc gcggtgttct atacttcgta gcgatagtat tgatcgtcaa cgtgatcaac
     5701 atccaccgcg aacataattt acaatgggtt tccggccacg gcaaacgaat gcatttgtgc
     5761 gcggggcaat gtaaataatt tttttaccat aaaatactac actatgtagt agaaaataaa
     5821 aaggagattc atgaaaacac tactcatgat cgcgtttctc gtgccatgca tcgggtgtcg
     5881 ggtcaatccc gatttggaag cggaaaaaac ggagctgtta cgcttacacg aagcacaacg
     5941 gcgtgcccat tttgatcgca acgtagatcc gatgacagaa gggcatgtac cgcaacgtat
     6001 tcaaatcagc ggaggagaat tgtcctacac cacgccggag caaacgcgtg cccggtttga
     6061 gcgttatttt ggtcgtgtcg aatttcttcg ttgggatgat acgatcgaac cgatcattgt
     6121 gatttctgat gatgctacgt tggccaatac atttgttcag aaactcgttg tggtgtctta
     6181 tcaggatgag cgcggcgcca aacaattgga tacaacccgg tttgcctgga caacgactta
     6241 ccgaaaaata aacgatcaat ggcagatgat cgctgtgact tccacccgaa aataagggga
     6301 tgaatatggt acgaatcgga ttgttttgtt ttttgtttca ttccgcggtt gctcaaaatg
     6361 atattatgct tttgtatcgt gatgcgatgg cattgatggt gaaaaaaaat tttgaacatg
     6421 cggaacagaa actccgaata ctggacagtc ttatgccgga tgacacggaa atttattttc
     6481 tccgagcgca ggttttagca cagatgggtg atcataccaa agcaatgggc ctattgaagg
     6541 cgagtctaat catgggcagc gatatgcttt tcgcaatcgg aaaagacagc gcatttgtgg
     6601 gtttgtgggc cgactccgag tttcaaaatt tactcaaaga aatggaacag cttaaacggc
     6661 ccgtccatca cagccgtgat gcttatcttc ttacagagcg ggatttgata cccgagggca
     6721 cagcctacga tggacatacg gggcaacttt ttatcagcag cacctataaa ggaaaaatca
     6781 aacgcattta tccggacggg cgcagcgatg attttactaa ggaaggccaa gacggcttgc
     6841 tcggcgtagt cgggatggag gtggattccg tgcggcgaat tttatgggtt aatagctgta
     6901 ataatccgaa tatgccgata cgggatagcc atgccgatgc acgggttcct gcgcgggtgc
     6961 ataaatttga ccttacgaca ggacgtctgc tgcagcggta tgatgctccg gcgggttcac
     7021 atttttttaa cgatcttacc gtcacggcga aaggggatgt ttttatcacc gataccgaat
     7081 cgggagaaat ctggcagatc gccgctgaac gccatacgct agaaatattt ttgaagctgc
     7141 agaacgtgct ttttctcaat ggcattacca agtcgccgga cgatcaatgg ctttatgttg
     7201 cccatgtggc gggaatttta gttatagata ttgctacacg ccggtatgaa cctttgaaac
     7261 atccgccgct attttcgctt ggaagcattg acgggctggc tttttacaaa caatcgc
//
LOCUS       DAOQNQ010000162         7298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3857_length_7298_cov_6.183626, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000162 DAOQNQ010000000
VERSION     DAOQNQ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3857_length_7298_cov_6.183626"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PK754_10275"
     CDS             <1..369
                     /locus_tag="PK754_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNB09935.1"
                     /translation="IIMAVMDFRSELQVLKSGFENGQLSVAEVTPQVEQIKSFVEEAM
                     RLGGVYPSSASKAHRSYYDILGIKEDASIDQIKAVYRKLSMIYHPDANAADDTGIVGD
                     ERFKEIKAAYEALLKDKQTL"
     gene            378..1643
                     /locus_tag="PK754_10280"
     CDS             378..1643
                     /locus_tag="PK754_10280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB09936.1"
                     /translation="MKLQKKTIVIFIVIVLLCLAQIFWWMYFQVQQNRTVMNLHKKMI
                     SSKALELRRFLTLEYRDLIQGSLNHPLHILRQAQENDWPRSHFVVTPDTIFLRYQDLI
                     YFFPINWPFLEQKLAASDSGFVLQKKPTISSAPATYLELPLTVIPKYKAEDNLQEQMD
                     RRTVMFLSEGSFFALMVMIAIYFLYAAFRRELQLQEHQKNFILSMTHELRSPLSSIKL
                     YLQTLQRRQITEERREQFVQHSLADVERLQRLVENVLDAARIERNDYDYQMGSLDMSQ
                     LVKSVLDKWKTSDTESVISFSGNIASDIHIHADAHAMISVVNNLIENAVKYSQAPKRV
                     NVLLSMIGDRAVLTVTDNGPGVAPEDRTKVFERFYRAGDEMTRTTKGTGLGLYIVKRI
                     VEAHRGTIDCQSAMEGGAAFTVSIPTLKK"
     gene            1657..2346
                     /locus_tag="PK754_10285"
     CDS             1657..2346
                     /locus_tag="PK754_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008504962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB09937.1"
                     /translation="MTNKSQILLVEDETHIAESLQFNLETEYNVIWAQTGEDALVQWK
                     KSKFDLIVLDVMLPKKSGYDVCAEIRKTDSETAILFLTAKGDARDRIEGLKLGADDYL
                     GKPFELDEFLLRVKALLKRRNAVNESSTSKFSFDKNEIDFENYIAVARGKKIQLTPKE
                     CMLMKLLIERQEQVVTREEILMKVWGYEAIPSTRTVDNFIVRLRNYFEVNPKDPTHII
                     SVHGVGYKFVP"
     gene            complement(2350..3354)
                     /locus_tag="PK754_10290"
     CDS             complement(2350..3354)
                     /locus_tag="PK754_10290"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNB09938.1"
                     /translation="MPTVAAWGQLRLELGFDRKNNLLWTTDPGIEKAAYSNLDIETWG
                     DFKLRNDLPQSPGGDGYLSLNGIQQYFQIPERAGLQVSGNISFSISMWLRVPAGTQNG
                     DIISSDNGFINGYRLFLEDGMIRFEIRDGTKEIFSSNRLLMPNQWQHIGVVCDGLGDS
                     VTFFINGASVASHPFTRVVQVQSGTQTYIGTRAESSFPNYLYGDIDDFRFFSGHDTIF
                     EYIRTMPERDLQRLSLKRPKTTTPSYFTLYQNYPNPFNMATRIDFEMIRPGSAELQIF
                     DLLGNMMKPIFTGELSAGRHTYTWDGTDNNNTPVSSGIYFARLTVNGIVQTKKMVLIK
                     "
     gene            3599..4054
                     /locus_tag="PK754_10295"
     CDS             3599..4054
                     /locus_tag="PK754_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNB09939.1"
                     /translation="MDDMEDDKKYSIHRLRGVDDDMDFIFSNLFNTKFPILLHSEKRW
                     HPPTDVLETDDEFIVVMDIANVKQDDIRLTYEAGILTVSGHRHEMKFSEKRHYHKMEI
                     DFGPFERRIKVNTRIQDQAIKAGYENGFLIVRLKKDTSRSSRVTNIVIE"
     gene            4064..6484
                     /gene="lon"
                     /locus_tag="PK754_10300"
     CDS             4064..6484
                     /gene="lon"
                     /locus_tag="PK754_10300"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNB09940.1"
                     /translation="MPEANISTDMKSDSIAIPEKLPILPLRDNVIFPFVVFPLVVMEK
                     NHVQLVQEALSSHKIIGVFTLRQPDKENPSFEDLYGIGTAVNVLKMFQVPDGSVRLLI
                     QGFSRIRLKAAMPDGLYLSGQVEPIHVPSADDLETDALKRGVSESFQKMVTLSPILPD
                     ELKVIVSNIREPDKLADMVASSLNIELNEKQEILESIGVKERLTKVMSAISREIQLLE
                     LNNKIQTEVNAELSKNQREYFLREQIKAIRHELGEDDDGSVEVEELKAAVEKKKMPAE
                     AKEAAMKEISRLSQMSPSAAEYTVSKTYVDWLLELPWYNATRDRIDLKKAKKILDEDH
                     YDLDDVKNRILEFLAVKKLRKDSKSPILCFLGPPGVGKTSLGRSIARAIGRKFVRFSL
                     GGVKDEAEIRGHRRTYIGALPGKIIQEIRRCQSSNPVFMLDEIDKIGQDFRGDPSSAL
                     LEVLDPAQNGSFNDHYVDIPFDLSDVMFIATANEISPIPAPLLDRMEVIRLSGYIAED
                     KLQIARKYIIPRQIEENGLKESDIEITDEAVYQIIASYTWESGVRNLEREIANVCRKI
                     ARKKAEGNKNKAKVQLPDIVEYLGPQKVFPEVARRTSETGVATGLAWTMNGGEILFIE
                     ARLMQGNRGMQMTGQLGNVMKESAQAAISYIRSKAGDLGIDPKIFDKNEIHMHIPSGA
                     TPKDGPSAGVTIVVCLTSLLTGKKVRHDIAMTGEITLRGKVMPVGGIREKVVAARRAG
                     IKTVILPKLNKNDLDDVPEYVKSSLTFIFADHIDDVLPHVFENDRTKKKSRASHKRSS
                     ATKRRRKG"
     gene            6495..>7298
                     /locus_tag="PK754_10305"
     CDS             6495..>7298
                     /locus_tag="PK754_10305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB09941.1"
                     /translation="MFNRIGKEQDSPESRAKLKVLCVDDEYVITNIMERILDEHYQVF
                     TASSGFEGLDILKQHPDTVVIVSDQKMEGMTGNEFLMKSQEIAPEAMRIMVTAYSDID
                     LVIDSINKGQIYKFIPKPFDAEHFRVTIHRAVDHYHHKIAYEKAFRELQTAQEGLVRA
                     EKLSALGKLLSDVSHELGGPIANINHAVTLAQHEWKDLKEFLDSVTSANFSAIDVQLL
                     MREKRVAELTHDFEQMMETIANASVFAMDLLSELKGFARNDDQQWIAVDI"
BASE COUNT         2137 a         1535 c         1735 g         1891 t
ORIGIN      
        1 attattatgg ccgttatgga ttttcgttcg gagcttcagg tgctcaagtc cggttttgaa
       61 aacggtcaac tgtccgtcgc tgaggtaacg ccgcaggttg aacagatcaa atcatttgtc
      121 gaagaggcca tgcgattggg tggagtttac ccctcatcgg ccagcaaggc gcaccgctcg
      181 tactatgata tactcggcat caaagaggat gcgagcattg atcaaatcaa agcggtatat
      241 cgcaaactga gcatgatcta ccatccggat gcaaacgccg ccgatgatac cggtattgtc
      301 ggtgatgaac gttttaaaga aataaaagcg gcttacgaag ccctgcttaa agataaacaa
      361 accttgtaaa caccgccgtg aaactacaaa agaaaaccat agttattttt atcgttattg
      421 tacttctgtg cctggcccaa attttttggt ggatgtattt tcaggtgcag caaaatcgta
      481 ccgtgatgaa tctgcataaa aaaatgataa gcagtaaagc attggagttg cgtcgttttc
      541 tgacattgga ataccgcgat ctgatacaag gatcgttgaa ccaccccttg cacatacttc
      601 gccaagccca agaaaatgac tggccgcgtt cacattttgt ggtaacgccc gatacgatat
      661 ttcttcgtta tcaggatctg atctattttt ttccgattaa ttggcctttt ctggaacaaa
      721 aattagccgc ttcggatagc ggatttgtac tgcaaaaaaa gcctacgatc agctctgcgc
      781 cggcaaccta tttggaattg cccttaacgg tgattcctaa atacaaagcc gaagataatt
      841 tgcaggaaca aatggaccga cggaccgtca tgtttttatc ggaaggaagt ttttttgcct
      901 tgatggtgat gattgcgatt tattttttat atgccgcatt ccggcgcgag ttacaattac
      961 aggagcacca aaaaaatttc attctgagta tgacgcatga attacgttcg ccgctttcat
     1021 cgatcaaact gtatttgcaa acgctgcaac ggcggcagat tacggaagaa cgccgggaac
     1081 aatttgttca acactccctt gccgatgtgg agcggttgca acgcttagtg gaaaatgtct
     1141 tggatgccgc ccgtattgaa cgtaatgatt atgattatca gatgggttcg cttgatatgt
     1201 cgcaactggt taaatccgtt ttagataaat ggaaaacatc cgatacggaa agcgtgattt
     1261 cgttttcagg taacattgcg tccgatattc acattcacgc cgatgcgcat gccatgattt
     1321 ccgtagtcaa taatcttatc gaaaatgccg taaaatattc acaagcacct aaacgggtta
     1381 atgtcttact gtctatgata ggcgaccgcg cggtattaac cgtaaccgat aatggcccgg
     1441 gcgtagcgcc cgaggatcgt acgaaagtat ttgaacgttt ttatagggct ggcgatgaaa
     1501 tgacgcgaac tactaaaggg accggtcttg gattgtatat cgtaaaacgt atcgtagaag
     1561 cgcaccgagg aactattgat tgtcaatcgg caatggaagg cggcgctgct tttacggtta
     1621 gtattccaac gcttaaaaaa taaaaggata ttaaatatga cgaataaatc gcaaatccta
     1681 ttggtcgaag acgaaaccca tattgcggaa tcattgcagt tcaatttaga gactgagtat
     1741 aatgttatct gggctcaaac cggggaggat gcgttagtgc aatggaaaaa aagtaagttc
     1801 gatttgattg ttttggatgt catgctgccc aaaaagagcg gttacgatgt ttgtgccgaa
     1861 atccgaaaaa cggattcgga gacggcgata ctttttttga ccgccaaagg tgatgcgcgc
     1921 gatcgtatcg aaggcctaaa actcggtgct gatgattatt taggtaaacc gtttgagttg
     1981 gacgaatttt tattgcgtgt caaagcttta cttaagcgcc gtaatgccgt caatgaatca
     2041 tcaacaagta aatttagttt tgataaaaat gaaattgatt ttgaaaacta catagctgtc
     2101 gcacggggaa aaaaaataca actgacgcct aaggaatgca tgctgatgaa actactgatc
     2161 gaacgtcagg agcaagtcgt gacgcgcgag gagatattga tgaaagtatg gggttacgag
     2221 gcgatacctt caacgcgaac ggtggataac tttatcgtac gtttgcgtaa ctattttgaa
     2281 gtaaatccca aagacccgac gcatattatt tctgtccacg gtgtcggtta caaattcgtt
     2341 ccgtagcgat tatttgatca acaccatttt tttagtttgt acaataccgt taacggtaag
     2401 gcgggcaaaa taaatgccgc tggataccgg tgtgttattg ttatctgtgc catcccaagt
     2461 ataggtatga cgccccgccg aaagttcacc ggtgaagatc ggtttcatca tattgcccag
     2521 caaatcaaat atctggagtt cagcgcttcc gggccgtatc atttcaaaat caatccgagt
     2581 ggccatatta aacgggttgg gataattttg gtacaacgta aaatatgacg gtgtggttgt
     2641 tttggggcgc ttgagtgata gacgctgtag atcgcgctcc ggcatagtgc gtatgtattc
     2701 gaaaatcgtg tcgtggcctg aaaagaagcg aaaatcatcg atatcgccgt acaaataatt
     2761 gggaaatgac gattcggcac gcgtgcctat ataggtttgt gttccggact gtacctgtac
     2821 tacgcgcgta aaaggatgcg aagcaacaga tgctccgttt ataaaaaaag taaccgagtc
     2881 gccgagcccg tcgcaaacaa cgccgatatg ttgccactga ttaggcatga gtaagcgatt
     2941 ggacgaaaaa atctctttgg taccgtcccg aatttcaaaa cggatcatac cgtcttccaa
     3001 aaataaacga tagccattga tgaaaccgtt gtcgctgctg ataatatcgc cgttttgtgt
     3061 gccggcggga acacggagcc acatagatat cgaaaaagat atattacccg aaacctgaag
     3121 accggcgcgt tcgggaattt gaaaatattg ctggatgccg tttagcgaaa gatagccgtc
     3181 gccgcccgga ctttgcggca gatcattgcg cagcttgaaa tcgccccacg tttcgatatc
     3241 aagattagag tatgcggctt tttctatacc cggatccgta gtccacaaaa gattattttt
     3301 gcggtcaaag ccaagctcca aacgcaactg tccccacgct gctacagtgg gcatgccata
     3361 aaaaaagcat attaataaaa agaaacgcgt agagtattgt gtagcaagga tcaaatccga
     3421 acctcacttt ccgttcattg gggtgaacaa gctaatgtag ccatggtata aacgataggc
     3481 aaatgataat tttttatgca cgatgaagct taaatgcgct tgcttttaca gtgtataact
     3541 gattatattt gtcctcgtat aagtaaaacc ctccgcgcaa tatccaaaag gtatttttat
     3601 ggacgatatg gaagacgata aaaaatattc catccaccgt ttacgcggcg tagatgatga
     3661 tatggatttt attttttcca acctttttaa taccaagttt cccattttac tgcactccga
     3721 gaaacgttgg cacccgccca ccgatgtctt ggagacggat gatgaattta tcgtagtgat
     3781 ggatatcgcc aacgtcaagc aggacgatat acgattgaca tacgaagccg gcattctgac
     3841 ggtgagcggt catcgtcatg aaatgaaatt cagcgaaaaa agacattatc ataaaatgga
     3901 aatagatttc ggcccttttg aacggcgtat caaagtcaat acacgcatac aagatcaagc
     3961 catcaaagcc ggttacgaaa acggttttct tatcgtacga ttaaaaaaag atacctcgcg
     4021 ctcatcgcgt gttacaaaca tagtgatcga ataataacta tctatgcctg aagcaaatat
     4081 atcaacagac atgaaatccg attccatagc gattccggaa aaactaccca tactcccgtt
     4141 acgggataat gtgatttttc cttttgtagt atttcctctt gtggtaatgg aaaaaaacca
     4201 tgttcagctt gtgcaagaag cgctttcgtc ccacaaaatc atcggcgtct ttactttacg
     4261 tcagcctgac aaagaaaacc cgtcatttga agatctctac ggtatcggta cggcggtcaa
     4321 tgtgcttaag atgtttcagg ttcccgatgg cagtgtgcgt ttgctcattc agggtttttc
     4381 gcgtattcgc ctcaaagcgg caatgccgga tggtctctat ctgagcggtc aggtggagcc
     4441 aatccatgtg ccatcagcgg atgatttgga aaccgatgcc ctgaaacgcg gcgtgtcgga
     4501 aagttttcaa aaaatggtta cgctctcgcc tatactgccc gacgaactca aagttatcgt
     4561 cagcaacatt cgtgagcccg ataagctggc cgatatggtt gcttcgagtc tcaatatcga
     4621 attgaacgaa aagcaggaaa tattagaatc tatcggcgtg aaagagcggc tcacgaaggt
     4681 aatgtccgcc atcagccgcg agatacaatt gctggagctc aacaacaaaa tacaaacgga
     4741 agtcaacgcc gaactcagca aaaaccagcg tgagtatttt ttgcgtgaac aaattaaggc
     4801 cattcgtcac gaattaggtg aagatgatga cggttcggtc gaagtcgaag agcttaaggc
     4861 ggccgtcgaa aaaaagaaaa tgcccgccga agccaaggaa gcggccatga aggaaatcag
     4921 ccgtctgtcg caaatgtcgc ccagtgcagc agagtatacc gtgagcaaaa cgtacgtgga
     4981 ctggctgctg gagcttccct ggtataatgc tacgcgggac aggattgatc tcaaaaaagc
     5041 caaaaagatt ttggatgaag accattacga tctagatgat gttaaaaacc gcatattgga
     5101 atttcttgcc gtcaaaaaat tacggaaaga cagcaaaagc cctatcctat gttttttggg
     5161 tcctccgggc gtcggtaaaa catcgcttgg tcgctcgata gcgcgggcta tagggagaaa
     5221 atttgtccgt tttagtctgg gtggtgtcaa agatgaagct gaaatacgcg ggcatcgtcg
     5281 tacctatatc ggagcattac cgggaaaaat aatacaagaa atccgccgtt gccaaagttc
     5341 aaatcccgtt tttatgctgg atgaaataga caaaatcggg caggatttcc gcggggaccc
     5401 gtccagcgcg cttttagaag tgctcgatcc ggcacaaaac ggttcattta acgatcacta
     5461 tgttgacatt ccttttgatc tgagcgatgt gatgttcatc gctacggcca atgaaatttc
     5521 cccgatacct gcgccgctgc tggatcgtat ggaagttatt cgtctgtcgg gttacatcgc
     5581 ggaggacaag ctgcagattg ccagaaaata tataatcccg cggcagatcg aagaaaacgg
     5641 attgaaagaa agcgatattg aaataaccga tgaagcggtg tatcagatca tcgcatccta
     5701 tacctgggag tcgggcgtac gtaatctgga acgcgaaata gccaatgttt gccgcaaaat
     5761 agctcgtaaa aaagctgaag gtaataaaaa taaagccaaa gttcagttgc cggatatcgt
     5821 cgaatacctc ggtccgcaaa aagtatttcc tgaagtggcg cgacgcacct cggagacggg
     5881 tgtagctacc ggtttggcat ggactatgaa cggcggcgaa attcttttta tcgaagcgcg
     5941 ccttatgcag ggcaaccgcg gtatgcagat gacggggcaa ttggggaatg ttatgaaaga
     6001 atcggcgcaa gcggcgatct catatattcg ttccaaagcc ggtgacttgg gtattgatcc
     6061 caagattttt gataaaaatg aaatacacat gcatattccg tcgggagcta cacccaaaga
     6121 cggcccgtcg gccggtgtga cgatcgtcgt gtgcctgacc tcgctgctta ccggaaaaaa
     6181 agttcgccat gatattgcca tgaccggtga aattactcta cgcggaaagg taatgcccgt
     6241 gggcggtatt cgtgaaaaag tggtcgccgc ccggcgcgcc ggtattaaga ctgtgatact
     6301 accaaagctt aataaaaacg atcttgatga tgtaccggaa tatgtgaagt catcgcttac
     6361 ctttattttt gccgatcata ttgacgacgt gctgccgcat gtatttgaaa acgatcgtac
     6421 taaaaaaaaa tcacgcgctt cgcataaacg atcatctgct acaaaacgac gccgcaaagg
     6481 atagtacacg tattatgttc aatcgtattg gcaaagaaca agattcgcct gaaagccgcg
     6541 caaaactcaa agttctatgt gtggatgatg agtacgtgat taccaatatc atggagcgta
     6601 ttctcgacga gcattatcag gtattcactg cgagcagcgg ttttgaaggc ttggatattc
     6661 taaaacaaca cccggatacc gtcgtgatcg tatccgatca aaaaatggaa ggcatgaccg
     6721 gcaacgaatt tctgatgaag tcacaggaaa ttgctcccga ggccatgcgt atcatggtta
     6781 cagcctattc ggatattgat ttggtgattg attcgatcaa taaaggtcag atttataagt
     6841 tcattcccaa accgtttgat gccgaacatt ttcgtgtgac cattcaccgt gccgtagatc
     6901 attaccatca taaaatcgct tacgaaaaag cttttcgtga attgcaaacc gcgcaggaag
     6961 ggctagtccg tgcggaaaaa ttatccgcgc tgggcaaact tttaagcgat gtaagtcacg
     7021 aactgggcgg cccgatagcc aatatcaatc atgccgtgac actggcccaa catgaatgga
     7081 aagatctgaa agagtttttg gatagcgtca cgtcggctaa tttttcggcg attgatgttc
     7141 agcttttgat gcgcgaaaaa cgtgtagcag agctcacgca tgattttgaa caaatgatgg
     7201 agacgattgc caatgcgtcc gtttttgcga tggatttatt gagcgaactt aaaggatttg
     7261 cccgtaatga tgatcagcaa tggatcgcgg tggatatt
//
LOCUS       DAOQNQ010000163         7248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3902_length_7248_cov_6.463228, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000163 DAOQNQ010000000
VERSION     DAOQNQ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3902_length_7248_cov_6.463228"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PK754_10310"
     CDS             complement(<1..254)
                     /locus_tag="PK754_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09942.1"
                     /translation="MNHITRIILIVSCCMSFALEAQTKADSLKNISLSGLQFRSIGPA
                     FAGGRIVDIAVNPANPAEYFVASGHGSLWKTTNGGTTFAPV"
     gene            586..660
                     /locus_tag="PK754_10315"
     tRNA            586..660
                     /locus_tag="PK754_10315"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:618..620,aa:Asn,seq:gtt)
     gene            728..1984
                     /locus_tag="PK754_10320"
     CDS             728..1984
                     /locus_tag="PK754_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09943.1"
                     /translation="MRKSLLGFWALFIIPFSLYSQLTGFHLYEKDGVYNVSPWLRYDR
                     ASGFFTGLTGDYFFTERLQMHGEGGYAWAARKPDYHLRLQQSFISDNTEYSIAAAYFD
                     KADTRDRMVLPEWQNSLTSIVAKRDYYDWYRIKGGNFEMRTDISGTLKITISGTWQHY
                     RNADVNAQWSMFDWGAKKNNGHARFRINPEIPEGRDVSLGTAIELDTRPSPLASVSAW
                     LIHSDYANARLLHKAFNSDFSYQRGSLTITRQQRMFGKQKWTFYGAYDGFSGNTVIQK
                     DSSSSLTYGQFLYDAGGLGSLRGYGYKEFSDGNRRMLFQSEYYFNGSFLPQTPLTKIW
                     GLGFIFKTFDLMLFWDIGHAWQASEKVMYFVPDGFHWRDMRSSAGGGLAVSDWIKIQY
                     AYVLKDGIRTHRGNYGIYFQFTPKLK"
     gene            1990..2721
                     /locus_tag="PK754_10325"
     CDS             1990..2721
                     /locus_tag="PK754_10325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNB09944.1"
                     /translation="MIDSQYFFVRPQDVYADHLVLRDDESQHCVKVLRKAPGDRFYAI
                     DGLGHEFTVALEHSDRTSAHCRILSTASKPRELGFEITLVQALIKKDHFDLVVEKVTE
                     LGVHRIIPVKTRRSLPEAGHTKIERWHKIALTAMKQSRRSLMPEIMPVSDLNSVLRRY
                     ADRPVQKIFLHESSEQPLDPNGINFAKACDTIIVVGPEGGFTDEEAAMAAEYGFQSFS
                     LGLRRLRAETASICGVGLFSMFAHP"
     gene            3009..3287
                     /locus_tag="PK754_10330"
     CDS             3009..3287
                     /locus_tag="PK754_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNB09945.1"
                     /translation="MTKADLVAKMAKDADVSKTKAEKSLESFIEAISMSLKKGKAVTL
                     VGFGTFSVAKRKARTGRNPQTGATIKIAAAKVPKFKAGKALKHVVKKG"
     gene            3426..4013
                     /gene="purN"
                     /locus_tag="PK754_10335"
     CDS             3426..4013
                     /gene="purN"
                     /locus_tag="PK754_10335"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNB09946.1"
                     /translation="MSKLILGILASGNGSNCRAIHKAITDGLCKAQIGVIISNHAEAG
                     VLQFARENGIPAFHIASEQFCEISEFHAAIKNCLSQHQVHLVVLAGYMKKIGKPILDI
                     YPNRIINIHPALLPAFGGKGMYGHHVHQAVLEYGAKITGVTVHFVDPEYDHGKIILQK
                     AVEVMEDDNVESLSARVLKAEHETYYKAINLFANE"
     gene            4066..5067
                     /locus_tag="PK754_10340"
     CDS             4066..5067
                     /locus_tag="PK754_10340"
                     /inference="COORDINATES: protein motif:HMM:NF027853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNB09947.1"
                     /translation="MKPIKNVLWLLIVGASLFAQGKTSIDSDKQKVAIIPIDAVKIAE
                     SYGATQTVNWQLSGATWKSAPESPFADPASLAAFAEAATQRVIEAFVKLGRFVVLDRS
                     AMEKIMKEQDFQLSDNVDPNTVTNIGGLLGAQYIVNGQLQQVSTNPYYNPKDKTTLMG
                     YSGTVEIQITILDVATGQVMTSKRVKGSTEVEGTKILGMALLDAYESTPIKAAYKGLS
                     ECGSDLKKWLRDAFPVEGEIFEITKEDKKKGAIVVNITCGKDIGVKKGDRFRVYTESE
                     VEIAGKMRKKTTDVATLAVKTVAEDGWSSLCDVEKGGKSISEKVKAGEKLKVVSVKK"
     gene            complement(5158..5730)
                     /locus_tag="PK754_10345"
     CDS             complement(5158..5730)
                     /locus_tag="PK754_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09948.1"
                     /translation="MRFPLFVFLVLLIASCGKKKTDESQLGQDSSSTAVQPINYDIPI
                     EAGRPVTSVKALPFREQLLAFSIELGNRSKDFNQRLGLILFAKRSGEPWREAVRNNIA
                     RENYRNAELYHTAYLDSLRGVIRPTDAALKSSYNELLIAHKRWRNNYQILHDHEKFES
                     MIAVLDTVLSNEMAINKALKDFKNTIDTKP"
     gene            complement(5733..>7248)
                     /gene="leuS"
                     /locus_tag="PK754_10350"
     CDS             complement(5733..>7248)
                     /gene="leuS"
                     /locus_tag="PK754_10350"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNB09949.1"
                     /translation="GAYAINPANGNPIPVWIADYVLINYGTGAIMAVPAHDTRDWEFA
                     KKFDLPIVEVVKAAKNVQEEVYDNNTDSVCVNSSNAEVSINGMNYKTAFDTIVAWIEN
                     KGIGKRTINYKLRDWLFSRQRYWGEPFPIVHLEDGRIMPLDESVLPLNLPEVQSYKPA
                     GTGESPLATMTDWVNITMDGKSARRETNTMPQWAGSCWYYLRYCDPHNDQAFVDAEKE
                     KYWLPVDLYVGGGEHAVLHLLYARFWHKVLFDLKLVSTPEPFMKLVNQGIILGEDSQK
                     MSKSRGNVVNPDDVIAQYGADAMRLYEMFMGPLTQTKPWSTRGVEGVYRFLQRTWRLF
                     VDERDQLSSKVQDVEPTPDQLRVLHKTIKKVQDDIEALSFNTAIAQMMIFVNELTPSD
                     VRPRKVIEPFLLLLAPFAPHIAEELWSRLGHTTSLTYEPFPVFDPAWTVEDTATVVLQ
                     VNGKLRDKLEVKKGLDKQTLETMAKEKLGHWVGGKEIVKIVVVPDKLVNVVVKG"
BASE COUNT         2097 a         1639 c         1583 g         1929 t
ORIGIN      
        1 accggcgcga acgtcgtccc gccattagtg gttttccaca atgacccgtg acccgaagca
       61 acaaaatatt ccgccggatt ggcaggatta accgcaatat ctacgatgcg gccgccggca
      121 aaagccgggc cgatactgcg aaactgcagt cccgataatg aaatgttctt gagcgaatcc
      181 gctttggttt gcgcttccaa cgcaaaactc atgcagcatg acacaatcag aataatacgc
      241 gtgatatgat tcataaaaaa atcctcatgc gatgggttaa ataataatga ttacttgata
      301 tgacaaatca ataaagcaat gaaaccgaga taccaaaaaa gaaaggacat aggcacgtca
      361 tgttacgaaa aacacaactt atagtttcgt ttaaatgctg gtttattttt aaaataagtc
      421 tatgaatttt taaatatgag aaaagaagct attacgatta gcattttaat acgaataatc
      481 cgaccgatga tgtggaaagc cgccaacctg agaatacatg gtaaagggaa aaaaaacttg
      541 acttttttga gtccgaacgc tattattgcc ccgttcgatg aacactgctc agtagctcaa
      601 tggcagagca ttcggctgtt aaccgaaggg ttgtaggttc gagccctacc tgagcagcca
      661 aacccgcgta cttgcattag gtatgcgggt tttctatttt gtgccaacat tttttgaaag
      721 cgtcgttatg cgaaaatcgt tgttagggtt ctgggcatta ttcatcattc ccttttcatt
      781 atatagtcag ttgaccggat ttcatctata cgaaaaagac ggtgtttata atgtttcacc
      841 ctggctccgt tacgaccgcg catccggatt tttcaccggc ttgacgggcg attatttttt
      901 taccgaacgc ctccaaatgc acggtgaagg aggatacgct tgggcggcac gaaaacccga
      961 ttaccacctc cgattacagc agagttttat atcagataat acggaatata gtatcgccgc
     1021 ggcatatttt gataaagccg atacgcgcga tcggatggtt ttaccggaat ggcaaaattc
     1081 tttgacatcc atagtggcca aacgcgatta ttatgattgg tatcgcatca aaggcggaaa
     1141 cttcgaaatg cgtaccgata tttccggtac actcaaaatc accatatctg gaacctggca
     1201 acactatcgc aatgccgatg taaacgcgca atggagcatg tttgattggg gtgcgaaaaa
     1261 gaacaatggc cacgcgcgat ttcgtataaa tccggaaatt ccagaagggc gtgatgtgtc
     1321 actcggtacg gcgatcgaat tagacacccg gccttcgcct ttggcatcgg tgagcgcatg
     1381 gttgatacac agcgactatg ccaatgcccg tttactgcac aaagcgttca attcggattt
     1441 ttcataccag cgtgggagcc tgaccattac gcgccaacag cgcatgtttg gaaaacaaaa
     1501 atggacattc tacggcgcct atgacggttt ttcagggaac accgtcattc aaaaagactc
     1561 ttcctcgtcg cttacttacg gccagttttt atacgatgcc ggcggcttag gcagtctgcg
     1621 cggatatggt tataaggaat tttcggacgg caatcggcgc atgcttttcc agagtgaata
     1681 ctattttaat ggttcttttt tgccgcaaac tcccctcacc aaaatatggg gcctgggttt
     1741 tatatttaaa acatttgacc tgatgttatt ttgggatatc ggccacgcat ggcaagcctc
     1801 ggaaaaagtc atgtatttcg tacccgatgg ttttcattgg agagatatga gatcatctgc
     1861 aggcggtggt ttggccgtat cggattggat caaaatacaa tatgcttacg ttttaaaaga
     1921 cggtatccga acccatcgag gaaattacgg aatctatttc caattcacac ccaagctaaa
     1981 ataatcgcta tgatagacag ccagtatttt tttgtccggc cgcaggatgt gtatgccgat
     2041 catttggttc tgcgagatga cgaatcgcaa cactgcgtca aagtgttgcg caaagcgccc
     2101 ggagatcgtt tctatgcgat agacggattg gggcacgaat ttacagttgc attggaacat
     2161 tccgatagaa catcagccca ctgccgtatt ctttcgacag cgagtaaacc ccgcgaactc
     2221 ggatttgaaa ttacactggt acaggctttg atcaaaaaag atcattttga cctcgtggtg
     2281 gaaaaagtga ccgaactcgg cgtacatcgt atcatccccg tcaagacgcg acgttctctg
     2341 ccggaggcag gccacacaaa aatagaacgt tggcacaaaa tcgcattgac cgccatgaaa
     2401 caatcgcgac gaagcctgat gccggaaata atgccggttt cagatttaaa ctccgtattg
     2461 cgaaggtacg cggatcgccc cgttcaaaaa atttttctgc atgaatcttc cgaacaacct
     2521 ctggatccaa acggaataaa ttttgcgaaa gcgtgcgata ccataatcgt cgttggtccg
     2581 gagggcggtt ttacggacga agaagccgct atggccgcag agtatggatt ccaaagtttt
     2641 tcgctgggct tacgccgttt gcgtgctgaa acggcaagca tttgtggtgt aggtcttttt
     2701 tcaatgtttg cacacccgta aaatatctaa aaacgggctt ttaggggcat atatacccgt
     2761 aatcatgcca ctatcaaggc tttatacgaa aatacatctt gacaaaagcc acttcacgat
     2821 atatattaat atcgttacaa aaaaacgatc tgttttcgat tcgaaatcaa aaacagacgc
     2881 tttcaaaaaa gttctttccc aatactaaat ttcatttcac gatcgctctc gatggttaga
     2941 ctgcttaagt tttgagtgcg tatgattaat caagtacgtg cttttcacca aaaaaaggag
     3001 ttctaaccat gaccaaagct gatctcgtcg ccaagatggc gaaagacgct gatgtatcga
     3061 aaaccaaagc agaaaaatct ttggaatcat tcatcgaagc gatctccatg tccttgaaaa
     3121 aaggcaaagc tgtaacgctc gttggtttcg gcacatttag cgtggcaaaa cgtaaagccc
     3181 gtacgggtcg caatccccaa accggcgcta cgatcaaaat tgctgcggct aaagttccga
     3241 aattcaaagc cggcaaagcc ctcaagcacg tggtaaaaaa aggttaatcg ctttcactcc
     3301 ctccgtaata ggtgaagaaa gcggtcacct aaagtctctc ggatataccg ggagactttt
     3361 ttattttttg gcttgattgc cttaccctca tcccatacat tttgccgaat gaaaactatg
     3421 ttctcatgtc taaactaatt cttggcatct tagcctccgg caatggctcc aattgcaggg
     3481 ctatccataa agcgataacc gatggattgt gtaaagctca aatcggtgta attatcagta
     3541 atcacgcgga agcaggcgtt ttgcagtttg ctcgtgagaa cggtattccg gcttttcata
     3601 tcgccagtga acagttttgt gaaatttcag aatttcatgc agcgatcaaa aattgtttat
     3661 cacagcatca ggtccatttg gttgttttag ccggttatat gaaaaaaatc ggtaaaccga
     3721 ttttagatat ttacccgaac cgcattatta atatacatcc tgcacttttg cctgcatttg
     3781 gcggcaaagg tatgtacgga catcacgtgc accaggctgt tttggaatac ggagcgaaaa
     3841 tcaccggtgt tacggtacat ttcgtagacc ctgaatatga tcatggtaag atcatcctcc
     3901 aaaaggctgt tgaagtgatg gaagatgata atgtggagtc cttgagtgct cgtgtactga
     3961 aagccgaaca cgaaacttat tataaagcga taaatctttt cgctaacgag tgatataaaa
     4021 atttactttc aaatactatt gaaccgaaat aacaggagag gcatgatgaa accgataaaa
     4081 aatgttttat ggctactgat agtaggcgca agtctgttcg cccaaggcaa aaccagcatt
     4141 gactcggaca aacaaaaagt agctattatc ccgattgatg cagttaaaat cgccgaatcg
     4201 tatggcgcta cacaaaccgt gaactggcaa ttgagcggag cgacgtggaa atcggctccc
     4261 gaatcacctt ttgctgatcc ggcatccctt gctgcttttg ccgaagctgc cacgcaacgc
     4321 gtcatcgaag cttttgtaaa gcttggccgt tttgtcgtgc ttgaccgtag tgcgatggaa
     4381 aaaataatga aggaacagga tttccaactt tccgataatg tcgatccgaa cacggtgacc
     4441 aatatcggcg gacttttggg cgcacaatac atcgtgaacg ggcaattaca acaagtcagt
     4501 accaatccgt attataatcc gaaagataaa acaacactga tgggctattc tggtacggta
     4561 gaaattcaga ttaccatctt agatgtggcc acaggtcaag ttatgacatc taagcgcgtc
     4621 aagggcagta cggaagtcga aggaacaaaa attctcggta tggcattatt ggacgcttat
     4681 gaatccacac cgatcaaggc tgcatacaaa ggtcttagcg aatgcggcag tgacctcaaa
     4741 aaatggctac gcgatgcatt ccctgtcgaa ggcgagattt ttgaaattac taaagaagac
     4801 aagaaaaaag gcgccatagt ggttaatatt acctgcggca aagatatcgg cgtcaagaaa
     4861 ggcgatcgtt ttcgcgtcta tacagaatcg gaagtggaaa tagccggaaa aatgcgcaaa
     4921 aaaaccaccg atgtcgctac tctcgcagta aagactgttg ccgaagacgg ttggtcatcg
     4981 ctgtgtgatg tcgaaaaagg cggtaaatcc atttctgaaa aggtcaaagc cggtgaaaaa
     5041 ctgaaagtag tttcggttaa aaaataactt caaataaata tctgaataaa aaaccccgaa
     5101 cacactcggg gtttttttat ttatcaaaac tatcaatatc tctatttcac gcatccatca
     5161 cggctttgta tcaatcgtat ttttgaaatc ctttagcgct ttgttaattg ccatttcatt
     5221 ggataagacc gtatccaaca cagcaatcat ggattcaaat ttctcatgat cgtgaagtat
     5281 ctggtaattg ttgcgccagc gtttatgagc gatgagcaat tcgttgtatg acgattttaa
     5341 tgcggcatcg gtcgggcgaa taaccccgcg aaggctatcc agataagctg tatggtacag
     5401 ttcggcattt ctataatttt cccgcgcgat attgtttcgc accgcctcgc gccacggctc
     5461 gccggaacgt ttggcaaaga gtatgagccc aagccgttga ttgaaatctt tactccgatt
     5521 gcccaattca atactgaaag cgaggagttg ctctctaaaa ggtaacgctt ttaccgaagt
     5581 aacaggacga ccggcttcaa taggaatatc gtagttgatc ggttgtaccg ctgtggatga
     5641 actatcttga ccaagctgtg attcatccgt ttttttcttc ccgcagctcg cgatcagaag
     5701 aactaaaaaa acaaatagtg gaaatctcaa actcatccct tcaccaccac attgactaat
     5761 ttatccggta ccacaactat tttaacaatt tcttttccgc caacccaatg tccaagtttt
     5821 tctttagcca tggtctctaa tgtttgttta tccaaacctt ttttcacttc aagtttatcc
     5881 cttaattttc cattgacctg aagaacaacc gtagccgtat cttccaccgt ccatgcggga
     5941 tcaaaaacgg gaaatggctc gtaggtgagt gacgttgtat gcccaagacg cgaccataat
     6001 tcctccgcaa tatgcggagc aaaaggcgcc aacaaaagca aaaacggttc tataactttt
     6061 cgcggccgca cgtccgacgg cgtaagttca tttacaaata tcatcatttg agcaattgcc
     6121 gtattaaaac taagcgcctc gatatcatct tgtacttttt tgatcgtttt atgcaaaacg
     6181 cgtaactgat ccggcgtcgg ttcgacatcc tgcactttag acgacagttg gtcgcgctca
     6241 tccacaaaaa gacgccatgt acgctgaaga aaacggtaca caccctcgac accgcgtgta
     6301 ctccagggtt tggtctgtgt caacggcccc ataaacattt cgtaaagccg catggcgtcg
     6361 gcgccgtatt gcgcgataac atcatcggga ttgacgacat tgcctcggga tttggacatt
     6421 ttttgcgaat cttcacctaa aatgatacct tgattgacaa gtttcataaa aggctccggc
     6481 gtagaaacca atttcaaatc gaacagcact ttatgccaga agcgtgcata taaaagatgc
     6541 aacacggcgt gttcaccgcc gccgacatat aaatcaacag gcaaccaata tttttctttt
     6601 tccgcatcca caaatgcttg atcgttatgg ggatcacaat aacgaagata ataccagcaa
     6661 gaaccggccc actgcggcat ggtgttcgtt tcgcgacgcg cggattttcc atccatggtt
     6721 atgttcaccc aatcggtcat cgtcgcaagc ggtgactcac cggtaccggc cggcttatat
     6781 gactgtactt ccggaagatt caacggtaaa acggattcat ccaacggcat gatacggcca
     6841 tcctcaaggt gaactatggg aaaaggttca ccccaatatc tctgacgcga gaaaagccag
     6901 tcgcggagct tatagttaat ggtgcgctta ccgatacctt tattttcgat ccaagctacg
     6961 atcgtatcaa atgcggtttt ataattcatt ccgttgattg atacttccgc attggaagaa
     7021 ttcacacaga cgctatccgt attattatcg tacacttctt cttgtacgtt tttcgccgct
     7081 ttaacgactt cgacgatggg aagatcaaat ttttttgcaa attcccagtc gcgtgtgtca
     7141 tgcgccggta cagccataat cgcaccggta ccatagttga tcaggacgta gtctgcaatc
     7201 cataccggta tcggattacc gttggcagga ttgatcgcat acgcaccg
//
LOCUS       DAOQNQ010000164         7247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3905_length_7247_cov_6.083704, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000164 DAOQNQ010000000
VERSION     DAOQNQ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7247
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3905_length_7247_cov_6.083704"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..696)
                     /locus_tag="PK754_10355"
     CDS             complement(115..696)
                     /locus_tag="PK754_10355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNB09950.1"
                     /translation="MRSLCVFCSSSDAVSDVHKRSAEELGAAIVHNGWSLIYGGTTVG
                     LMGLVAISVKSHGGHVVGVIPEVIAKHGISNAQADEMIVTRDLRERKAVMESRADAFV
                     ALPGGYGTLEEIFEIVTLRQLNAIQKPAALLNINGFYDAIGEFIRSASAGQFIKPEAV
                     GLCPLIPDVPSLFDYLQHYKPITISHKWFRDVN"
     gene            complement(709..1590)
                     /gene="speB"
                     /locus_tag="PK754_10360"
     CDS             complement(709..1590)
                     /gene="speB"
                     /locus_tag="PK754_10360"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNB09951.1"
                     /translation="MSKTPDNFLGIEKKLSDYKTAKFAVLPIPYEATTSYGQGTRKGP
                     EAIIKASQMVEFYDEELDRDSVYDFGIATLKPVTFGKNKGEKAMKQIQQAVGKVLDDQ
                     KMPVCLGGEHTVTSPIVRAFHDRLGDDFSVLQIDAHSDLRETYHDTPWSHACVMKRIW
                     DFNKNIVQIGIRAQCIEERKLIQQNNIQTFYAKDIVGGGDEWMLEAIHALKEKVYITI
                     DCDGLDPSIIPATGTPEPGGLEWYPTMSFLRKVCERRTVIGFDIVELAPHKQFPFSDY
                     ALAKMAYKLMGYVSAKK"
     gene            complement(1659..3767)
                     /locus_tag="PK754_10365"
     CDS             complement(1659..3767)
                     /locus_tag="PK754_10365"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HNB09952.1"
                     /translation="MASEQTLNVAFLWHQHQPYYKVAAPDGKPFFAMPWVRLHGVKDY
                     FDIPNYLTDFPTLKQNFNLVPSLLMQIEDYTERGVEDHIMRLTRLPAGQLSEHDREQI
                     IDDFFLAHEERMIAPNPRYKELLDRRSEWRQFSVEDWCDLQMWYNLCWIGESWKTKEP
                     FRSWIQKGRGFSESDKAGVLAAHLDILRKIIPLYRRLAREQQIEISVTPLYHPILPLL
                     NDTAIAQKSNPHTTLSEKAFVYPNDAHWHIRAAREFFQSRMGFAPRGMWPSEGSVSQA
                     VIEQIAEEGFRWVATDEEILMRSESRTPGDPYQPYRIRTSKSEVVVLFRDHGLSDAIG
                     FRYAAMPPEKAAAEFVSHLEAIREDLIRRGLDPSQRLVSIILDGENCWEYYPNNGREF
                     LRALYLRLSTSQTIETVRINDYLMKIKTPQLPEIHHIHPGSWIAHNFDVWIGSHPEKN
                     TAWEYLTETRLFLETARKSGKYTFEKLDHAWEMMAIAEGSDWFWWMGDDHQARHKTRF
                     DVLFRYFLRRVYDALGEPAHEKLFVPIMADRKKEPVLHRPKALLRPRIDGKESSFYEW
                     RDAGFYAASKDADTMSGGQVWFDSIQFGFDEHYLYIRLNFSPDQITLLREKKFTVQAF
                     FDYEGQRVVHAQEVNAHTQDWVFGEIAEWRIIRSRIPLPCRFSVGLFDGRNNLETKPL
                     RGGIPLDPDHDAMDRDVWSA"
     gene            complement(3874..4548)
                     /locus_tag="PK754_10370"
     CDS             complement(3874..4548)
                     /locus_tag="PK754_10370"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB09953.1"
                     /translation="MLTPRKHLTKKELHKDPVMNKISQFTEYVQTNQQVILYGIVGLL
                     VIIATSWGYMNYSKERDAESVNKLAAPERVYFSGDYREAIRRLEKYCAEYEGTVGGGM
                     GTFYMANAYFNTDQYDFALQYFNQYLDDYADNELFVMSSLMGIAACYEGQNKYEEAAK
                     QYEKAAGKFPSSHLAPESMIGAARCYRMVNQASNAKALYEKVTKEYPETSAARDAKNA
                     LDEIGA"
     gene            complement(4865..4938)
                     /locus_tag="PK754_10375"
     tRNA            complement(4865..4938)
                     /locus_tag="PK754_10375"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4902..4904),aa:Arg,seq:cct)
     gene            complement(4957..5553)
                     /locus_tag="PK754_10380"
     CDS             complement(4957..5553)
                     /locus_tag="PK754_10380"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNB09954.1"
                     /translation="MSDSPSPLSEETPVVENTTANDTVASVSEDLDAMEKPAEEQAKT
                     ETPDYETQIAALKDQLVRALADHDNFRKRKAKEFQELILTANERLLTDILPVLDDMER
                     AMKAAKDVPTDDPKVQQFVQGFEFIYKKTMKILTEKGLKPMESVGKILDPNLHDALMQ
                     LDMPDKESHTIVDEHEKGYYLNDKVIRHAKVIVSKKPE"
     gene            complement(5599..6654)
                     /gene="hrcA"
                     /locus_tag="PK754_10385"
     CDS             complement(5599..6654)
                     /gene="hrcA"
                     /locus_tag="PK754_10385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNB09955.1"
                     /translation="MISLSEREYIVLRKIVQSFIENGTPIGSRTLSKILPWELSAATI
                     RNIMADLEDKGFLTHPHTSAGRIPTDRAYRAYVDAMDGPISLPAEDMATISDAVLALA
                     RDISRIQDGVDEILYHSSRALAKISNELGIILAPQFHQSVFDRMRFSEVAHDRVLIEL
                     SLRSGFVKTVILDVRDSLSREHLHEISLAINERLSGLTVDEIRKSFGERLRDLRYRFS
                     DRQEALVRVLLNSAAQLFNFSTRQSVTMAGTNNILAKPDFVGRTEMQTMFALLEEKER
                     VAGILAERSTDRVIVTIGEENGVSSINGCSIITAPYRMGEGAGTVGLIGPKRMPYDKL
                     IPLVEHTARVLDKAFSI"
     gene            complement(6659..>7247)
                     /locus_tag="PK754_10390"
     CDS             complement(6659..>7247)
                     /locus_tag="PK754_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09956.1"
                     /translation="VWAATGGGLGRFDGTEWTPYTHTNSGLLDDAAQCVVVDAAGNKW
                     IGTQDGGVFKFKDTTWVGYDTTSYSPIKMPVVNIKSIAIEKSGILWLGTARGLMRFDG
                     KKIELYNTKNSGIPDNLIRGLAIDGAGNKWIATTKGLVKFDGTKWTTYTIANSKLPAD
                     RVTAVFVGDKDHIWVGCEGWGLTVFRETGVTLTEE"
BASE COUNT         1861 a         1816 c         1583 g         1987 t
ORIGIN      
        1 cctttggaat ttaacctaag gatttgtttt atctaatttc agcgaattta ggcgttttaa
       61 aaagtgaagt caagttattt tattaagtta cgttaaacgg acggcggccg tatttcagtt
      121 cacgtcgcgg aaccatttgt gtgaaatagt gatcggtttg taatgttgca gataatcaaa
      181 aagtgaaggc acatccggga ttaatgggca caaacctacc gcttcgggct tgataaactg
      241 accggcactg gccgaacgaa taaattcacc gatcgcgtca tagaacccat tgatattgag
      301 cagcgccgca ggcttttgta tggcattgag ttggcgtaat gtaacaattt cgaatatctc
      361 ctccaacgta ccgtacccgc cgggcaaagc cacaaacgca tcggcacgcg attccatcac
      421 ggctttacgt tcacgcaaat cgcgcgtcac gatcatctca tccgcttgtg cattactgat
      481 accatgcttg gctatcactt cgggaataac accgactaca tgtcctccat ggcttttgac
      541 actgatcgct acaaggccca ttaagccgac ggtcgttccg ccatagatca gtgaccatcc
      601 gttatggacg atcgccgctc ctaattcttc cgcggatcgc ttatgtacat cgctaacggc
      661 atcactggac gaacaaaaca cacataatga acgcatcacg cgtaaacctc attttttagc
      721 agacacatag cccattaatt tataagccat tttagctagt gcataatcgg aaaacgggaa
      781 ctgtttgtgg ggcgccagtt ccacgatatc aaaaccgatc accgtgcggc gttcgcatac
      841 tttgcgaaga aacgacatgg tgggatacca ttccaaaccg cccggctcgg gcgtcccggt
      901 cgccggtata atactgggat ccagtccatc gcaatctatc gtgatataca ctttttcttt
      961 caatgcgtga atcgcctcga gcatccattc gtccccgccg ccgacaatat ccttcgcgta
     1021 aaaagtttga atgttgttct gctgaatcag tttccgttcc tcgatacatt gcgcgcgaat
     1081 accgatttga acaatgtttt tattaaaatc ccaaatacgc ttcataacac atgcgtgact
     1141 ccacggcgta tcatggtacg tttcgcgcaa atccgaatgc gcgtcaatct gtagtacgga
     1201 gaaatcatcg cctaagcgat cgtggaatgc gcgtacgatc ggcgaggtta ccgtatgttc
     1261 gcccccgagg catacgggca ttttttggtc atctaaaact tttccaacgg cctgctggat
     1321 ttgtttcatc gctttttcac ctttgttctt tccaaaggtt accggtttaa gcgtggcaat
     1381 gccaaagtca tatacgctat cgcgatccag ctcttcatca taaaactcaa ccatctgcga
     1441 agctttaatg atcgcttcgg ggcccttacg cgttccctga ccataactgg ttgttgcttc
     1501 gtaaggaatc ggcaaaacgg caaatttggc cgttttgtaa tccgataatt ttttttctat
     1561 gcccaaaaag ttatcgggtg ttttgctcat gatatttctc cgaaattaaa cgaggtgatt
     1621 tttataaatc tatgccggtt accatacatc aaaatatatc atgcagacca tacatcacga
     1681 tccatcgcgt catgatccgg atcgagtgga ataccgccgc gcaagggctt ggtttccaga
     1741 ttattacgcc catcaaacag acccacggaa aaacgacacg gtaaaggaat gcgcgaacgt
     1801 attatgcgcc actctgcaat ttcaccgaag acccaatcct gagtatgtgc attcacctcc
     1861 tgtgcatgca ctacgcgctg gccttcataa tcaaaaaaag cctgtacagt aaattttttt
     1921 tcgcgcaaca aagtaatttg atcgggtgaa aaattcaacc ggatgtagag ataatgttca
     1981 tcaaatccga attgaatgct atcgaaccaa acttggccgc cgctcatggt atcggcatct
     2041 tttgatgcgg cataaaaacc ggcatcgcgc cattcgtaaa acgaagattc tttgccgtca
     2101 atacgcggtc ggaggagcgc tttggggcga tgcagcacag gttctttttt acgatcggcc
     2161 atgatcggaa caaataattt ttcatgcgcg ggttcgccca atgcgtcgta tacgcgacgt
     2221 aaaaaataac gaaacagcac atcaaatcgc gttttgtggc gcgcctgatg atcgtcgccc
     2281 atccaccaaa accaatcgct gccctccgcg atagccatca tctcccatgc atgatccagt
     2341 ttttcaaacg tatattttcc gctcttgcgc gccgtttcga gaaataaacg cgtttccgtg
     2401 agatattccc acgccgtatt tttttccgga tgcgaaccga tccagacgtc gaaattatga
     2461 gcaatccatg aacccggatg aatgtgatgt atttccggca attgcggcgt ttttattttc
     2521 atcaaataat cattgatgcg aacggtctct atggtttgcg acgtggatag gcggagatat
     2581 aaagcgcgta ggaattcgcg accgttgttg ggataatatt cccagcaatt ttcgccatcc
     2641 agaataatgc tcaccaatct ttgcgacgga tccagcccgc gacgtatcag atcttcgcga
     2701 atggcttcca aatgcgacac aaactcggcg gcggcctttt ccggcggcat ggcggcataa
     2761 cgaaatccga tcgcatcgga gagaccgtga tcgcggaata gcacgactac ttcggacttg
     2821 gaagtacgga tgcgatacgg ttgatacgga tcgcccggag ttcgcgactc ggagcgcatc
     2881 aaaatttctt catctgtcgc aacccatcgg aaaccttctt cagcgatttg ctcaatcaca
     2941 gcctgactga cgctgccttc ggacggccac atcccgcgcg gtgcaaaacc catgcgcgac
     3001 tggaaaaatt cgcgtgcggc acggatatgc caatgtgcgt cattcggata aacaaatgct
     3061 ttttccgaaa gcgtcgtatg cgggttggat ttttgcgcga tggccgtatc gttcaataac
     3121 ggcaaaatcg gatggtacag cggtgtgacg gaaatttcga tttgttgctc gcgcgccaac
     3181 cgccggtaga gaggaatgat ttttcttaga atatccaaat gcgccgctaa aacgccggct
     3241 ttgtcggatt cgctaaaacc gcgccccttt tggatccacg aacgaaacgg ttctttcgtt
     3301 ttccacgatt cgccgatcca gcacagattg taccacatct gaagatcgca ccagtcttcg
     3361 acagaaaact gccgccactc ggagcgtcgg tctaataatt ctttataccg cggattgggt
     3421 gcgatcattc gctcttcgtg ggcgagaaaa aagtcgtcta tgatttgctc gcgatcatgt
     3481 tcgcttaatt gaccggccgg caagcgcgtc aatcgcataa tatgatcttc gacaccgcgt
     3541 tcggtataat cttcaatctg catcagcagc gaaggcacca aattaaaatt ttgtttgagc
     3601 gtagggaagt cggtgaggta attaggaata tcgaaataat cttttacacc atgcaggcgc
     3661 acccaaggca ttgcaaaaaa tggtttgcca tccggcgcgg ccactttgta atacggctgg
     3721 tgctggtgcc acaagaaagc gacgttaaga gtttgttccg atgccatcgc ttgtgaagtt
     3781 ttataaataa tgaaaaaccg taaaacaata tacgaataaa aaaaatgggt tgccacaaaa
     3841 gcaacccatt aatataactt ttaaaattta cgcttaggcg ccgatctcat ccaacgcatt
     3901 cttagcatca cgcgcggcgc tggtttcagg atattctttg gtcacttttt catacaacgc
     3961 tttagcattt gacgcttgat tgaccatgcg gtagcaacgc gctgctccga tcatgctttc
     4021 gggtgccaaa tgggaactgg gaaatttacc agctgctttt tcgtattgtt tggccgcttc
     4081 ttcgtattta ttctgccctt cataacaagc cgcaataccc ataagcgacg acatcacaaa
     4141 aagttcatta tccgcatagt cgtcgagata ctggttgaaa tactgtaacg caaaatcgta
     4201 ttgatcggta ttaaaataag cattggccat ataaaaagta cccatgcctc ctccgacggt
     4261 accttcgtat tcagcacaat acttttcgag acgacgaatc gcttcacggt aatcaccgct
     4321 gaaataaaca cgctccggtg cggctaattt attgacggac tctgcatcac gttctttact
     4381 ataattcata tagccccaag acgtggctat aatgaccagt aaaccgacaa taccgtacag
     4441 gataacctgt tgattggtct gcacgtattc ggtgaactgt gaaattttat tcatcaccgg
     4501 atctttatgc agttcttttt ttgtaagatg ttttcttggc gttaacatgc gtaattcact
     4561 tcattctgtg gtctaattga tttttaaagc gccccataat acaaaacggg cttcttgcac
     4621 gcaagtaaaa ttaacagaaa ctaattttta ttctagttgg ttaaaaatga ttctacggct
     4681 caataagtcc tctatcaata ttgtgttgtt acaattataa catcaagcct gcggaaacga
     4741 cgaccattga tcttgcgctg attgtaccct caatcacgcc gttggtgtga cgaccattga
     4801 tcttgcgatg attgtaccct caatcacgcc gttggcgtga tgaccattga tcttgcgctg
     4861 attgtaccct cgacacgatt tgaacgtgcg accaacggtt taggaaaccg ctgctctatc
     4921 cagctgagct acgagggcga atatcgaata aaaaacttat tccggttttt tactgacgat
     4981 cactttggca tgacgaatga ctttatcgtt gagataataa cctttttcat gctcgtccac
     5041 gatggtatgg gattctttgt ccggcatatc cagctgcatc aatgcatcgt gcagattggg
     5101 atctaatatt tttccgaccg actccatagg ctttaaacct ttttccgtca gtatcttcat
     5161 cgtcttttta tagataaatt caaagccttg tacaaattgc tgcaccttag gatcatccgt
     5221 aggtacatct tttgctgctt tcattgcgcg ttccatatca tccagcacag gaagaatatc
     5281 agtcaacaac cgttcattgg cggtcaggat gagttcctga aattctttgg ctttacgttt
     5341 tctgaaatta tcatgatccg ccagcgcacg caccagttga tctttcagcg cagcgatctg
     5401 ggtttcataa tccggggttt ctgttttagc ttgttcctcg gcaggttttt ccattgcatc
     5461 cagatcttcc gacacggatg caacggtatc gttagcggtc gtgttttcta cgacgggtgt
     5521 ttcttcggat aagggggacg ggctgtcaga catagtttta aaaacgtgtt ttaattggtt
     5581 ggtgaaatag ttatcagttt aaattgaaaa agctttatcc aatacacggg ccgtatgttc
     5641 gacgagcggt attagtttat catacggcat acgtttagga ccgatgagtc ccacggttcc
     5701 ggcgccttca cccatacggt aaggcgccgt gatgatgctg cagccattga tcgaagatac
     5761 gccattctct tctccgatcg tcacgattac gcggtccgtc gagcgctcgg ccaagatccc
     5821 cgctacgcgc tctttttcct ccagtaaggc aaacatcgtc tgcatctccg tacgtcctac
     5881 aaaatccggt ttggctaaaa tattattcgt ccctgccatc gtcacggact gacgcgtgct
     5941 aaaattaaat agctgcgccg ccgaattgag caacacacgt accaacgctt cttggcggtc
     6001 actgaaacga taacgtaaat cccggagtcg ttcaccgaat gatttgcgaa tttcatctac
     6061 ggtgagaccg ctcaggcgtt cgttgatggc caaactgatc tcgtgcaagt gttcgcgcga
     6121 caaactgtca cgcacatcaa gtataaccgt tttgacaaat ccgctacgca gggaaagttc
     6181 gatcaatacc cggtcgtgtg caacttccga aaaccgcatc cgatcaaata cgctctgatg
     6241 aaattggggt gcgaggataa tgccgagttc gttagaaatt ttggccaacg ctctggagga
     6301 gtgatataaa atctcgtcaa caccgtcttg tatgcgtgaa atgtcacgtg cgagcgccaa
     6361 tacggcatcc gatattgtag ccatgtcttc agccggtaac gatatcggtc catccatcgc
     6421 atccacatac gcacggtatg ctcgatctgt cggaatacga ccggccgatg tgtgcggatg
     6481 cgtaagaaaa cccttgtctt ccagatccgc cataatgtta cgtatcgtcg ccgcactcaa
     6541 ttcccacggc aaaattttgg acaacgtgcg tgaacctatc ggcgttccgt tttcgataaa
     6601 cgattgaacg attttgcgta acacaatgta ttcgcgttct gagagagata tcatcgcatt
     6661 actcttctgt caatgtcacg ccggtttctc tgaataccgt gagtccccag ccttcgcagc
     6721 ccacccaaat atgatcttta tcaccgacga aaacagccgt cacacgatcc gccggtagtt
     6781 tcgaattggc aatggtatat gtagtccact ttgtcccatc aaacttgaca agacctttgg
     6841 tcgtagcgat ccatttgttt ccggcaccgt caatcgccaa accccgaata agattatccg
     6901 gaatgccgga attcttcgta ttatacaatt ctattttttt tccatcaaat cgcatcagac
     6961 cgcgcgccgt accaagccat aggatgcctg acttttcgat cgcaatactt ttgatgttga
     7021 ccaccggcat tttgatgggg ctataggacg tcgtatcgta accgacccat gtcgtgtctt
     7081 taaatttgaa tacaccgccg tcttgtgtgc cgatccattt atttccggct gcatccacga
     7141 caacacattg agccgcgtca tccaaaagac cggaattggt atgagtgtac ggcgtccatt
     7201 cggtaccgtc gaagcgccca agtcctccgc ccgtcgccgc ccacact
//
LOCUS       DAOQNQ010000165         7243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3911_length_7243_cov_5.489983, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000165 DAOQNQ010000000
VERSION     DAOQNQ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7243
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3911_length_7243_cov_5.489983"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..928)
                     /locus_tag="PK754_10395"
     CDS             complement(158..928)
                     /locus_tag="PK754_10395"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNB09957.1"
                     /translation="MSNALHDDIRRLLIQEQDLYGDEFTRDPAASGLWNHVSWDTPEY
                     STTDDERPPVVAERIETDMPKNIPLPTLSALQQTISTCTKCELCKTRTQTVFGSGNPN
                     ADIMFIGEAPGADEDEQGLPFVGRAGQLLTKMIEEPRSLNLPRSEVYIANILKCRPPG
                     NRKPLPAEVAQCEPYLITQIELIKPRIICALGATSANTLLKNNTPIGAMRGQTFDYHG
                     VPLLITYHPAALLRNPNWKKPAWEDMLTLRAMYDKLKK"
     gene            1120..1737
                     /locus_tag="PK754_10400"
     CDS             1120..1737
                     /locus_tag="PK754_10400"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNB09958.1"
                     /translation="MRPITTMFWDLGGVVLTNAWDREQRIKVLNSHGITEKQHLDEFG
                     DRHREVAAGFETGHYSLDEYLDMTLFDMHHVAREPFKESMFGQSELLPGMDVLTEVAQ
                     AGRCFMATLNNESLPLNEYRIARFKLKQYFQVFCSSCYLGVTKPTKEIYETALRITHS
                     KPEECVFIDDREQNAEAAKKLGIHAIHYKNPDQLRKELRFLKIID"
     gene            1746..3584
                     /locus_tag="PK754_10405"
     CDS             1746..3584
                     /locus_tag="PK754_10405"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNB09959.1"
                     /translation="MSFLRIDQVKHIIGFSQHVMRSVAILIFLSYSALQAQTVQCVAS
                     VEQSVIEQGTSFQLRIEVSGENLRITSLPQLPEMKGLEVMHQGAGESTSISIINGRRS
                     MSKSYDFILRAKDVGRWTIPPVAVGVDGKTVSSQALTIEVVPGGSISGSQRSEKTNDA
                     FLALTVSKKEAFQGEQITAELKIYSRIPITQYAPSRMPGFTGFWAEEFPMPQQIQSTR
                     EQINGVAYAVYTIKRVGLFATRSGLLTIDPSEIECELRIPRRSRNSLDDLFSPFGDPF
                     GQTATVKLQSEPVKLNILPLPKENAPSDFSGLVGQFDVKTAVDKNSLKTGEALTYTIT
                     ISGTGNIKSAEIPPTPFGDDFERYDPKSSETLNKNSNLVSGSKVFEYVGVPRLPRQVE
                     IPVFNLSYYDPVLKKYVTKSSGPHRVTIAQGTGGGIFSHNALSKEEIALLAKDIRFIK
                     TENLSEESYAPVYFRWWFIAGMIFPLLLWGTVYVWAIKHEELQKDGIGLRYRKASPLA
                     RKRLKAAEALMKSNQQDSFYTELSKTLNGLITDKLHVGENVYTREHITEILRERNVEN
                     NLITEYMDCMHMCDEARFSPDGKSRARMDDAYERIRAAILNMDNAL"
     gene            3581..4333
                     /locus_tag="PK754_10410"
     CDS             3581..4333
                     /locus_tag="PK754_10410"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB09960.1"
                     /translation="MNRFLFWIVMSAAPVLWAQENDTLFAKAHAFYEARDFVAARDAY
                     QKLVDQGSVSGALFYNLANTYYRTKQFGMAVFYYEKALRLHPADEDVRFNLELTRLQL
                     KDKIVTPPRPEWVVWMIATLQAISLAEWGFVLVCIVNGFVVLLVAQRVRPQWRRVRTG
                     RILRMVWITLLCINASVFLWRAYSESNRVEAVILVPYVEIKNEPIETSTTVFILHEGT
                     KAVIRSERNEWLELRLDDGNVGWIKRPAVGIL"
     gene            4335..6728
                     /locus_tag="PK754_10415"
     CDS             4335..6728
                     /locus_tag="PK754_10415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HNB09961.1"
                     /translation="MRSDTSVYPALKLTILYIVSILILDAVSPEIHWPINKAIVSSLV
                     VLTGIVLYWRFQNHFWGVIAVFFLVLGRMPDNQFQTHHVSSYLDLNQKVLVHGTVVND
                     PQINTQRTRFAVETDSVVFTRNLILPSSGKIQVVCFTDSTVRPAYGDRISIWGYLRSP
                     RGSRNPGEFDFAAYAAYHGLYGVMYAGDAANIRIEGHTSLNPVTEFFILPIKHFIIEL
                     NEKTLPPLHAAIASGLIVGDRSDIPQEILNAFSYSGAIHILSISGLHVVFISLLILGL
                     FSVLRVPYVWRIILTLISLFLYVWIADFVPPAVRAGIMTGVVLFGTLLERRTNIINSL
                     CVSLLIILFWDPLAIFDIGLQLSFLAVLSLVIIYPRLEAWCKKRRILTERTPSVLRWV
                     IMGLLVSIAAQIGTLPFTAYYFYKIPLTALIANVFISPISSAVMALGFLSAIVGSFSF
                     TLAQWYAHLNDYLIGLMIATARFSVKLPLAYTQFYRMDMIVLSVYYLSLAVWFFWDRK
                     LVRRWAVWVVIAALSVGVIFQNIPTSKKTVITFLDVGQGDACVVHLDDGRTIVIDAGD
                     QDPQFDYGERVVAPYLWKQGISSIDALILSHPHDDHIGGAGFLLNHFDVKRVFDPGQY
                     YPSAIYKNILNTIAEKSIDYQVLQKDRVVTFGPETRIRILHPGVSFVRSDESPPYDIN
                     NTSLVFVLEYGENRILFSGDAETETWDALSDSGMAMKADIVKIAHHGSSNGTTAKLIE
                     AASPAYAVISCGEGNKFGHPDLETVNAWRNAGTQVLRTDQWGAVQFWLDGKSIQRTR"
     gene            6732..>7243
                     /locus_tag="PK754_10420"
     CDS             6732..>7243
                     /locus_tag="PK754_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09962.1"
                     /translation="MLGYDLIVLLGFFVGAILYSVLFAETLKRQNKLKHEYVFLVFVA
                     ILTFWHGFQFLGMLIKQVFGRPALEIYSELRYISLYAIAFFPPLVVHLHASFLEYVLN
                     LINRRSRILAWFPYIMYAPILAILYFSQYTIIDGRRRILDEGMSFIPPYVFWLIVCLL
                     VSGFLSSRIIR"
BASE COUNT         1763 a         1583 c         1807 g         2090 t
ORIGIN      
        1 cagcttgaac gactgcatcg gccaattgat cagcgagaaa aacttcgctg ccgtagagca
       61 gatacatcgg agcgacgttt cctttgcgca cgtctttgag gacatcattg tacgaggcgt
      121 ttttttcttt ttcttcgcgg gccatgagaa gtggtgttta ttttttcaat ttgtcataca
      181 tggcgcggag tgtgagcata tcttcccaag ccggtttttt ccaattgggg ttgcgcagca
      241 aagcggccgg atggtacgtt atgagtaaag gtacgccgtg ataatcaaac gtttgtccgc
      301 gcatcgcacc aatcggcgtg ttgtttttca gcaacgtatt ggcggatgtc gcaccgagtg
      361 cacaaataat acgcggcttg atcaattcga tctgcgtgat gagataaggt tcgcactgtg
      421 ctacttcggc gggtaaaggt ttacggttgc ccggcggtcg gcacttcagg atattggcta
      481 tatacacttc cgaacgcggc agattgagag aacgcggctc ttcgatcatc ttcgtcaaaa
      541 gctgaccggc gcgacccacg aaaggtaaac cttgttcatc ttcatcggca cccggcgctt
      601 caccgataaa catgatgtcg gcattcggat tgccggagcc aaacactgtt tgcgtgcgcg
      661 ttttgcacaa ttcgcatttg gtgcaggtac tgatggtttg ctggagagcg gataacgtcg
      721 gaagggggat gtttttgggc atgtcggttt cgatccgctc ggcgacaacc ggcggccttt
      781 catcatcggt ggtagaatat tccggcgtat cccatgagac atgattccaa agaccggaag
      841 cggcggggtc gcgggtaaat tcatcgccgt acaaatcctg ttcttgaatc aacaatctac
      901 ggatatcatc gtgcaaggca ttactcatga tcacactata accatattcc gcttttttat
      961 caagcgaaac gccatgccag gaaagaggaa cgttaatgac ggaaacggat acgctcctta
     1021 gtaaattctt ttagcatata ggtattgaaa aaagcctgtg cactgtataa gttccctcgt
     1081 ttcaatttta aaaaaacact taattgaaag aacacaacta tgcgacccat cacgacgatg
     1141 ttttgggatc ttggcggagt agttttgacc aatgcttggg atcgtgagca gcgaatcaaa
     1201 gtgctgaatt cgcatggaat tacggaaaaa caacatttag acgaattcgg cgaccgtcat
     1261 cgtgaagtcg ccgcaggatt tgagaccggg cattattcgc tggatgaata tcttgatatg
     1321 acgctcttcg acatgcatca cgtcgcccgc gagccgttta aagaatccat gtttggtcaa
     1381 tccgagcttc taccgggaat ggatgtgctg acggaagtcg cacaggcagg ccgttgtttt
     1441 atggccacat taaacaatga atcgctgccg ctgaacgaat accgcatcgc gcgtttcaag
     1501 ctcaaacagt attttcaggt gttttgttct tcatgttatt tgggtgttac caaaccgacc
     1561 aaagaaattt acgaaacagc gctccggatt acacatagta aacctgaaga atgcgttttt
     1621 atagatgacc gtgaacaaaa cgccgaggca gccaagaaac tgggcataca cgcgattcat
     1681 tacaaaaatc cggaccaact gcgcaaagaa ttgcgttttc tcaaaattat agattgaatc
     1741 ctattgtgag ctttttgcgt atagatcagg taaaacacat catcggcttt agtcaacacg
     1801 tcatgcgaag tgtagcgatt ttaatttttt tatcgtattc ggcgttgcaa gcgcagaccg
     1861 tccaatgcgt ggcttctgta gagcagtccg tcatcgaaca aggtacgtca tttcaactgc
     1921 gcatcgaagt gtcgggtgaa aacctgcgca tcacatcgct tccccaattg cctgaaatga
     1981 aaggccttga ggtcatgcat cagggtgcgg gcgaatccac gtccatatct attatcaacg
     2041 gtcgtcgctc catgtccaaa tcgtacgatt ttattttgcg tgcgaaagat gtgggtcgct
     2101 ggacgatccc tcctgtggcc gtgggtgtgg acggaaaaac ggtgtcctcg caggcgctca
     2161 cgatcgaagt cgttccgggc ggaagcatat ccggttccca gcgttcggaa aaaactaacg
     2221 atgcgtttct cgcattgacg gtttccaaaa aagaagcgtt ccaaggtgaa caaattacgg
     2281 ccgaactaaa aatttacagc cggattccga tcactcaata cgcgccaagc cggatgccgg
     2341 gttttacagg attttgggct gaagaatttc ccatgccgca acaaatacaa tctacccgtg
     2401 aacaaatcaa tggcgtggcg tatgcggtat atacgatcaa gcgggtaggg ctttttgcaa
     2461 cgcgatccgg tttgctaacg atagatccgt cggaaatcga atgcgaactg cgcattccgc
     2521 ggcgcagcag aaactcgttg gatgatttat tttctccgtt cggcgatcct ttcggacaga
     2581 cagctacggt caagttgcaa tcggaacctg tgaagctgaa tattctcccg ttgcccaaag
     2641 aaaatgcgcc gtcggatttt tcgggtttgg tcggacaatt tgacgtaaaa actgcggttg
     2701 acaaaaacag tttgaaaacg ggtgaagcgc tgacgtatac gatcactata tcggggacag
     2761 gaaatattaa atccgccgag attccaccga cgcctttcgg tgatgatttt gagcggtacg
     2821 atccgaagtc gtccgaaacg ctgaataaaa attctaatct cgtcagcggc agtaaagttt
     2881 ttgaatatgt cggtgtgccg cggctaccgc gccaagtgga gataccggtt tttaatctct
     2941 cttattacga tccggttttg aaaaaatacg ttactaaatc atcaggtcct caccgtgtga
     3001 ccatagcaca aggtacaggg ggcggtattt tttcgcataa cgcgctgagt aaagaggaaa
     3061 tcgcgttgtt ggcgaaagac attcgtttta tcaaaacgga aaatctttcg gaagaaagtt
     3121 atgcgccggt ttattttcgc tggtggttta tcgcgggaat gatatttccg cttcttttgt
     3181 ggggtacggt atatgtgtgg gcgattaaac acgaagaatt gcaaaaagac ggtatcggtt
     3241 tgcgatatcg caaagccagt cctctggcgc gtaaacgtct caaagcggct gaagcgttga
     3301 tgaaatccaa tcaacaggat tcgttttata ccgaactttc caaaacatta aacggcctta
     3361 tcaccgataa attgcatgtc ggtgaaaatg tgtacacccg cgagcacata acagaaattt
     3421 tgcgcgaacg gaatgtggaa aacaatttga taacggaata tatggactgc atgcacatgt
     3481 gtgatgaagc gcgtttttca cccgacggga agtcgcgcgc acggatggat gatgcgtacg
     3541 agcgtattcg tgcagctatt ttaaatatgg acaacgcgct atgaatcgtt ttttgttttg
     3601 gatcgtgatg agcgctgcac cggtgttatg ggcacaggaa aatgataccc tgtttgcgaa
     3661 ggcgcatgct ttttatgagg cgcgtgattt tgtagcggcc cgtgatgcat accaaaaact
     3721 tgttgatcaa ggttcggttt ccggcgcgtt gttttataat ttagccaata cctattaccg
     3781 gacgaaacaa tttggtatgg ccgtgtttta ttacgaaaaa gcattacgtt tgcatcctgc
     3841 cgacgaagat gtacggttta atctcgaatt gacacgactg cagcttaaag ataaaatcgt
     3901 aacaccgccc agaccggaat gggtcgtatg gatgatcgct acgcttcaag cgatttcgct
     3961 tgccgaatgg ggttttgtgt tggtctgtat tgtaaacgga tttgtcgttt tattggttgc
     4021 tcaaagagta cgcccgcaat ggcgaagggt gcgcacgggc cggatactgc gcatggtatg
     4081 gatcacactt ctttgtatca acgcatctgt ttttttatgg cgtgcctaca gcgaatccaa
     4141 tcgggtcgaa gccgtgattt tggttcctta tgtggaaatc aaaaatgaac cgattgaaac
     4201 atcaacgacc gtattcatac tgcacgaagg cacgaaagct gttattcgat cggagcgcaa
     4261 cgaatggttg gaactgcgtt tggatgacgg aaacgtggga tggataaaac gaccggctgt
     4321 cggcattctc tgatatgcga agcgatacat cggtttatcc cgcgctcaag cttaccattc
     4381 tctatatcgt ttccatactt attctcgatg cagtatcccc ggaaatccat tggccgataa
     4441 ataaagccat tgtctcttcg cttgttgttt tgaccggtat cgttttgtat tggcgcttcc
     4501 aaaatcactt ttggggtgtc atcgccgtgt tttttcttgt attgggccgt atgcccgata
     4561 accaatttca aacgcatcac gtttcatcct accttgattt gaatcagaaa gtattggtac
     4621 acgggactgt cgtcaacgat ccgcaaatca acactcagcg aacacgtttt gcggtggaaa
     4681 ccgactcggt cgtttttacc cgaaatttaa ttttaccgtc gtctggaaaa atccaggttg
     4741 tatgctttac tgattcgacc gttcgcccgg cgtatggcga tcgcatttcg atttgggggt
     4801 atctgcgttc tccgcgcgga agtcgtaatc cgggagagtt tgattttgcg gcgtatgctg
     4861 cataccacgg attatatggt gtcatgtatg ccggcgacgc ggccaatatt cgcatcgaag
     4921 gtcatacgtc actgaatccg gtgacggaat tttttatctt accgatcaaa cactttatca
     4981 ttgagctcaa tgaaaaaacg cttccgccgc tgcatgccgc catcgccagt ggtcttatcg
     5041 tcggtgatcg cagcgatata ccgcaggaaa ttcttaatgc tttttcgtat agcggcgcga
     5101 ttcacattct ttcgatcagc ggtttacatg tggtttttat ttcgctttta atccttggac
     5161 tatttagtgt cttacgtgtg ccgtacgtat ggcgtattat tcttacgctg atcagtcttt
     5221 ttttgtatgt gtggatagcc gattttgtgc cgccggcggt gcgtgccggt atcatgaccg
     5281 gcgtcgtgtt attcggtaca ttattggaac gccgaacgaa tattatcaat tcactgtgcg
     5341 tttcgctgct gatcattctc ttctgggatc ctttggcgat ttttgatatc ggattacaac
     5401 tctcattttt agcggtgttg tcattagtta ttatctatcc gcgtctggaa gcatggtgca
     5461 aaaagcgacg aattctcacg gaacgtaccc cgtcggtgtt gcggtgggtc attatggggt
     5521 tattggtttc gatagcggca caaatcggta ctttaccgtt tacggcatat tatttttata
     5581 aaattccgct tactgcgttg atagcaaatg tttttatatc accgatttca tcggcggtga
     5641 tggcgttggg atttttgtcc gcgatagtcg gttccttttc gtttacgctt gcccaatggt
     5701 atgcccatct caacgattat ctgatcggat tgatgatcgc cacggcccgg ttttccgtta
     5761 aacttcctct cgcctacacg cagttttacc gcatggatat gatcgtattg agcgtttatt
     5821 atttatcgct ggccgtgtgg tttttttggg acaggaaatt ggttcgccgc tgggccgttt
     5881 gggttgttat tgcagcttta agtgttgggg tgattttcca aaatataccg acttctaaaa
     5941 aaacagtgat tacattttta gatgtgggtc aaggcgatgc gtgtgtcgta catctggatg
     6001 atgggcgtac gatcgtgatc gatgcaggag atcaggatcc gcagtttgat tatggcgagc
     6061 gggttgtcgc gccttatttg tggaaacaag ggatttcttc gattgatgcg ttgattttat
     6121 cgcatccgca tgacgatcac atcggcggcg ccggattttt actcaatcat tttgatgtaa
     6181 aacgggtttt tgatccgggt caatattatc cgtcggcgat ttataaaaat attttgaata
     6241 ccattgcgga aaaatctata gattatcaag ttttgcaaaa ggaccgggtt gttacctttg
     6301 gtccagagac gcgaatacgt atactgcatc ccggggtatc gttcgtgcga agcgatgagt
     6361 cgccgccgta tgatatcaat aatacgtcgc ttgtatttgt tttggaatac ggcgaaaatc
     6421 ggattttatt tagcggggat gcagagaccg aaacgtggga tgcgttgtcc gattccggga
     6481 tggcgatgaa ggccgatatt gtcaaaatcg cacatcacgg ttcttccaac ggtacgacgg
     6541 caaaattgat cgaggccgcg tctccggcat acgcggtaat atcttgcgga gaaggaaata
     6601 aattcggtca tcctgatttg gaaaccgtaa atgcctggcg caatgccggt acgcaggtct
     6661 tacgaacgga ccaatggggc gctgttcagt tttggctcga tggtaaatcc atacaacgca
     6721 ctcggtaaac tatgttaggc tacgatctta tagttctgct cggttttttt gtcggcgcga
     6781 tactttatag cgtgctgttt gccgagactc tcaagcgtca gaataaactg aagcatgaat
     6841 atgtgtttct ggtattcgtg gcgatactta cgttttggca cggatttcag tttctcggaa
     6901 tgctcatcaa acaagtattt ggccgcccgg cgctggagat ttactccgaa ctccgttaca
     6961 tctctttgta tgcgatagcg ttttttcctc cgctcgtcgt gcatctgcac gcctcgtttt
     7021 tggaatacgt gctcaatctg atcaaccgcc gctcacgtat attagcatgg tttccctaca
     7081 ttatgtatgc gccgattttg gcgatacttt attttagcca atacacgatc attgacggac
     7141 gtcgccgcat tctggatgag ggcatgagtt ttataccgcc ttatgttttt tggctgatcg
     7201 tatgtttatt ggtttccggc tttttatcgt cacgcattat acg
//
LOCUS       DAOQNQ010000166         7212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3937_length_7212_cov_6.895627, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000166 DAOQNQ010000000
VERSION     DAOQNQ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7212
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3937_length_7212_cov_6.895627"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PK754_10425"
     CDS             complement(<1..255)
                     /locus_tag="PK754_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09963.1"
                     /translation="MAVSSGKKKTTKTVKPKAVKSDTAEKPVKEKSVKKTKTPVAEEK
                     TAVESAPAKRTRGGRKPFVRAKTVKEDIEITIPDNIERTNK"
     gene            complement(341..1585)
                     /locus_tag="PK754_10430"
     CDS             complement(341..1585)
                     /locus_tag="PK754_10430"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB09964.1"
                     /translation="MKRSPLTVIFITVFIDLVSFGIVIPLLPFYAQQFDASGTTIGLL
                     LSVFSLMSFLFMPLWGRISDRYGRRPVLLVTIFCSVIGYIIFANAFALWLLFLARIMS
                     GIGNANISVAQAYISDVTSAKERAKGMGLIGAAFGLGFVFGPLIGGVFSGEAFGAYRF
                     AIPGWIAVGLSLINFFMAYFMLPESLAQTKQDEIKAHHLIDVRGLQRAIKKENLGLLM
                     IFFFIVTIAFSSIYATFPLFIMEEPFRLTSSAMGWFFVEIGIVSVIIQGGLIGRLNHR
                     FGEQRLISFGAIAMAIGFITFPLSAYLPIGGIVLLIISTALISMGSSCLAPTTMGLTS
                     RLADVSEQGSILGIAQSFASLGRMIGPFIGGAAYDYAGHKSPYYFAFVLMAVAAVMAF
                     ILKSRDLKLDEAPVAMEPVAEV"
     gene            complement(1593..2921)
                     /locus_tag="PK754_10435"
     CDS             complement(1593..2921)
                     /locus_tag="PK754_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006667922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB09965.1"
                     /translation="MSRHPALTPSEKKSIWGWCMYDWANSAFILTVVTAVLPVYFADV
                     VVPKEGFDIGGTLYSATSLWGFMISSSALFVFLCAPMLGAIADFTAAKKKFLMVFCYT
                     GSLFTVLLFFCGSGDVWMTMIFLIIAQIGFVGGNIFYDAFLPHVAPPGQMDWVSGKGF
                     AYGYIGSDIQFLLCLGLILGHDALGLTGTMAVRLVLAFSGLWWAGFSIFTWKYVHEAP
                     ATENIPDDLRSSPKVIAYIRLGLRRTVGVLKKIVSTPYLLIFLIAFMLYNEGIQTTIV
                     MATIYGKEELGLSTSVLMITLFVIQIVAMFGALLFSKLAERWSTQKAILTTLVGWSLV
                     VLYAYGMTTSAEYMALGVVVGIVMGGSQALSRSYYGAMIPKESSAEYYAFYSVFTKFS
                     AIWGPLVFAVIRQATGSSRLSIVSLIVFFILGMILLTMVRVPKSAEVPAS"
     gene            3042..3743
                     /gene="purQ"
                     /locus_tag="PK754_10440"
     CDS             3042..3743
                     /gene="purQ"
                     /locus_tag="PK754_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNB09966.1"
                     /translation="MKTGVVVFPGSNCDHDAFYTAGELIGHKAEYIWHKDRKDLRSFD
                     LIIVPGGFSYGDYLRTGAIARFAPIMQDVMDFAKYGGTVFGVCNGFQILTEAGLLPGA
                     LLRNSHLHYVCKNIYLRAENDNSRFTNTLKKGEVLMVPIAHGEGNYFADNETLKRLEG
                     DNRVLFRYCTENGTINADGNPNGSLNNIAGIMNENGNVAGMMPHPERCADPALGQNDG
                     LKVFESLLIARQKAA"
     gene            complement(4179..5120)
                     /locus_tag="PK754_10445"
     CDS             complement(4179..5120)
                     /locus_tag="PK754_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09967.1"
                     /translation="MNKFVLAFISIVLCGCSGCTDDKKKPYEGFLDKDREFVIAHVAP
                     DEDRINPVEVFVGRLLTIVPDSTDGANIQDYLLKIQLRQFQNEYYALLKYHDDAQVFI
                     TSSGQTVELQNVGKGTYRDVSNALHISALQTCTLKVTRPGGKVYQAMVRVPDHITITS
                     LSPGDTTVLYPKKNTPESDFCGAVAYISGPAVTGAYIYRSKFDETAHLQSSNNSGSAV
                     IIELCGGVEYTNISETVMAFDTVLTRAWGMGEDTGFGYDLFDSLLHWRSAATPITYRS
                     NITNQDGAKIAGYFGAYNSINTHFVVKSLRDSCQCNN"
     gene            complement(5186..6328)
                     /locus_tag="PK754_10450"
     CDS             complement(5186..6328)
                     /locus_tag="PK754_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09968.1"
                     /translation="MKKFPLLMVFVMIASCTDHQQMDNASLEDVEIRETLNEMAQTLA
                     SDPSWIDQTLIEKLKKGAVKSADLPSSIKLSKSNGFVQDIEIEMYDNSNVKSLSKSLG
                     TTPYYVGLDPDVYNFSPSIQEELTGLPVMDMPVFYFDGQSVSETTVQLDPDKQTDKLI
                     FFIKSTSLEPHTLSKSMQIAGTFFACRSIKVKVKQDESDEEFEVYFSNGSHPINSYFN
                     GTTDHVFNGNTREDAAGNSRYYMDVNDKNLWYPTSDGQEISLIRVDNLTDSVRMVAIE
                     ADWSSGNYVQGRYNRNQQVGTTSEILTDYYDMTDNTKKLQENSAYYVDLKTSLNDDRY
                     QKGAIKSINARTLYARTNNGEHYIETANADGGALTDVDWIIKQIVQ"
     gene            complement(6838..>7212)
                     /locus_tag="PK754_10455"
     CDS             complement(6838..>7212)
                     /locus_tag="PK754_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HNB09969.1"
                     /translation="WRLPATAILVCAVLGFYNGFFGPGTGSLLVFAFVSVIGFSFLKS
                     SAMAKIVNVVADISSLIFFITNGFVFWKLAIPMAFCQIAGSVVGSRMAVLKGNRFIRY
                     VFLVVVGGLILRFGYDVWKMMA"
BASE COUNT         2172 a         1728 c         1472 g         1840 t
ORIGIN      
        1 tttattggta cgctcaatat tatcgggtat tgtgatctca atgtcttctt tgaccgtctt
       61 agcacgaaca aacggtttac gaccgccgcg tgtacgtttg gcgggtgcgg attcgacggc
      121 tgttttttcc tccgcgacgg gggttttggt ttttttaacc gatttttcct taaccggctt
      181 ttccgcggta tccgatttga cggctttagg cttaacggtt tttgtagtct tcttttttcc
      241 ggatgatact gccataatta tgaatttctc tcaatcgtta tacaaaaagc ggattcaatt
      301 tatcaaaaaa cgatggaaat gcaagatggt gcgaaaaaaa ctatacttcg gccaccggtt
      361 ccatcgcaac cggcgcctcg tccaatttca gatcacggct tttgagaata aaagccatga
      421 ccgcggcaac ggccatcagc acaaaagcaa aataatacgg tgatttatgt ccggcatagt
      481 catacgccgc accgccgatg aacggaccga tcatccgtcc taaactggca aaactctgtg
      541 caataccaag aatcgagcct tgctctgaca catcggccaa acgcgatgta agacccatcg
      601 tcgtcggcgc taaacacgaa ctgcccatcg agatcaacgc ggtcgaaatt ataagtaaaa
      661 caataccacc gattggcaaa tatgccgaca aaggaaaagt aataaaaccg atcgccatag
      721 cgatagctcc aaaactgatc aaacgctgtt caccaaagcg gtgattcagt ctgccgatca
      781 aaccgccttg aatgattaca ctcacgattc cgatttctac aaaaaaccat cccatcgccg
      841 aactcgtcag acgaaatggc tcttccataa taaacagagg gaacgtcgca tagatactgg
      901 aaaaagcaat cgtcacaata aaaaagaaaa tcatcagtaa gcccaggttt tcttttttta
      961 tggcgcgctg taaaccgcgc acatctataa gatgatgcgc ttttatttca tcctgcttgg
     1021 tttgtgccag agattccggc agcataaaat aagccatgaa aaaattgatc aacgacaacc
     1081 ccactgcgat ccaaccggga atcgcaaaac gatacgctcc aaacgcttcg ccggaaaaaa
     1141 caccgccgat taacggccca aagacaaatc caagaccaaa tgccgcgccg atcaatccca
     1201 tacctttggc gcgttccttg gccgaagtga catcgctgat ataagcttgt gccacggaaa
     1261 tattcgcgtt accgatcccg gacatgatac gtgccaaaaa aagcaaccac agtgcgaacg
     1321 cattggcaaa aatgatatat ccgataactg aacaaaatat cgtcaccaat aaaaccggcc
     1381 ttctcccgta acgatccgaa atacgtcccc ataaaggcat aaataaaaat gacatgagtg
     1441 aaaaaacact caataacaaa ccgatcgtcg tgcccgatgc gtcaaactgc tgcgcataaa
     1501 acggtaataa cggtattaca ataccaaaac ttaccagatc aataaatacc gtaataaaaa
     1561 taaccgtaag cggagaacgc ttcatacaac tcctatgatg caggcacttc ggctgacttg
     1621 ggaactcgta ccatcgtcag caatatcata cccagaataa aaaaaactat tagcgataca
     1681 atggataaac gggacgaacc ggtcgcttgc cgaatgacgg caaacacaag cggaccccat
     1741 atcgcagaaa acttggtaaa tacagaataa aacgcataat actcggcaga agattctttg
     1801 ggaatcatcg caccgtaata cgaacggctc agcgcttgcg acccacccat gacgatgccg
     1861 accaccacgc ccagcgccat atattcagcc gaagtagtca tcccatacgc atacaagacg
     1921 accaacgacc acccgaccaa tgtcgtaaga atcgcctttt gtgtactcca acgttcggcg
     1981 agtttactga aaagtagtgc accgaacatg gccacgatct ggatcacaaa aagtgtgatc
     2041 atgagtaccg aagtcgagag accgagttct tctttcccgt agatagttgc catgactatc
     2101 gtagtttgaa taccttcgtt atacaacata aaagcgataa gaaaaattaa cagatacggc
     2161 gtggaaacta tttttttaag tacaccgacc gtccgacgta atccgagacg aatatacgct
     2221 atgactttgg gcgaacttct taaatcatca ggtatgtttt ccgtcgcagg cgcttcgtgt
     2281 acatatttcc aagtaaaaat agaaaacccg gcccaccaca aaccggaaaa tgccagtacc
     2341 aaacgaactg ccatcgtacc ggtcaatccc aacgcatcgt gtcctaaaat aagaccgagg
     2401 cataacaaaa actggatgtc gcttccgata taaccatacg cgaaaccttt cccggatacc
     2461 caatccatct gcccgggcgg agcgacatgc ggcaaaaaag cgtcataaaa aatgtttccg
     2521 ccgacaaaac cgatttgggc gatgatcaga aaaatcatag tcatccatac atcaccggac
     2581 ccgcaaaaaa acagaagtac ggtaaaaaga ctaccggtat aacaaaacac catcagaaat
     2641 ttctttttgg cagcggtaaa atcagcgatc gcacctaaca tcggtgcaca aagaaataca
     2701 aatagcgccg agctgctgat cataaagccc cacagcgatg tcgcactata cagtgtgccg
     2761 ccgatatcaa aaccctcttt gggtacgacg acatcggcaa aatacaccgg caataccgcc
     2821 gtgacaacgg taagtatgaa ggccgaattg gcccaatcgt acatacacca cccccaaatg
     2881 gatttttttt cagagggcgt taacgcagga tgccgggata tcaatcgtta gctccgtttt
     2941 tgtagtcgcg caaaataagc gatcatccgt cgaatgtcaa attgcaattg cacaatgttt
     3001 ggcgatgttc tatattcccc gcattcaaaa aaggagttat catgaaaacg ggtgttgtcg
     3061 tatttccggg ttccaattgc gatcacgatg ctttttacac ggccggtgaa ctgatcggcc
     3121 acaaagcaga atacatttgg cacaaagacc gtaaagacct tcgatcgttt gatctcatca
     3181 tcgtacccgg cggtttttcg tatggcgact acctgcgaac cggcgccatt gcccgtttcg
     3241 cgccgatcat gcaggatgtg atggattttg caaaatatgg cggtaccgtt ttcggtgtct
     3301 gcaatggttt tcagattctg accgaagccg gtttactgcc cggggcctta ttgcgcaaca
     3361 gccacctgca ctacgtgtgt aaaaatattt accttcgcgc ggaaaatgat aattcgcgat
     3421 ttaccaatac gctgaaaaaa ggcgaagtgc tcatggtgcc gatagcgcac ggtgaaggca
     3481 attattttgc cgacaacgag acgctcaaac ggcttgaagg cgacaaccgt gttctttttc
     3541 gctattgcac ggaaaacgga acgatcaacg ccgatggcaa tcccaatggt tctctgaata
     3601 atattgccgg tatcatgaat gaaaacggta atgtcgccgg tatgatgccg catccggagc
     3661 gttgtgcaga tccggcttta ggtcaaaatg acgggctcaa agtttttgaa tcgctgctta
     3721 tagcgcggca aaaagcggca taatttttta cgccatcatt ttccaaacat cataaccgaa
     3781 acgaagtatc aatccgccga cgacgacgag aaatacgtat cggataaacc gattgccttt
     3841 caatacagcc attcggctgc ccaccacttt gtacagatca ttgtcgaaat gaagtgaagc
     3901 gaattccgga acgatatgat cgcaaaatgc aatttatata atgtaaccca taaaattaaa
     3961 ccttacgatt tcctctatcg cgaactaaat tctttaaact tttctaacaa gattttatcg
     4021 ttttgattct cgacaacttt atttacatgc ttatcaagtt attcattttc acgcacaaat
     4081 gttttattaa acttttttca tgaccatgtt tttacatcac ttatttaatt ttctgaatca
     4141 agcccgaaat aatttctgca taaatccgat aagcagattt aattattaca ctggcatgaa
     4201 tcccttagcg atttcacaac aaaatgcgtg ttgatggaat tataggcgcc gaagtagccc
     4261 gcgattttag ctccgtcctg atttgtaata ttcgaacggt acgtaatcgg agtggccgcc
     4321 gatcgccaat gcaaaagtga gtcgaacaaa tcatacccga acccagtatc ttcacccatg
     4381 ccccatgctc tagtcagtac cgtatcaaag gccatgacgg tctcggatat atttgtgtac
     4441 tccacacctc cgcataattc gatgattacc gcagaaccac tattgttaga agactgtagg
     4501 tgcgcggtct catcaaactt agacctgtaa atataggcgc cggtcaccgc gggccctgat
     4561 atataagcga ccgcaccgca gaaatcactc tcgggcgtat tcttcttagg atagagcacg
     4621 gtcgtatctc cgggagacaa actcgtgatc gtaatgtgat ccggaactct gaccatcgct
     4681 tgataaacct taccccccgg cctagtgacc ttgagcgtgc aggtttgaag cgccgagatg
     4741 tgtagcgcat tggatacgtc acgataggtc cccttaccca cgttttgaag ctcgaccgtc
     4801 tgacccgaag acgttataaa tacctgtgcg tcgtcgtgat acttcagcag cgcatagtat
     4861 tcgttttgaa actgacgtaa ctgaattttc aaaaggtaat cttgaatatt ggcaccgtcg
     4921 gttgagtcgg gaacaatggt caacagtcgt cctacgaaaa cttcgacagg attgatccga
     4981 tcctcgtccg gggctacatg tgcgatgaca aactcacgat ctttatccaa gaagccttca
     5041 tatggctttt ttttatcatc cgtacaaccg gaacatccgc atagtacgat ggatataaaa
     5101 gctaaaacaa atttattcat agggctccta aaataacaaa ctatccggtc gcaaaaccgg
     5161 atagtctgaa aagaaaaaag attaattact gtacaatctg cttaataatc caatctacat
     5221 ctgtcaaagc gcccccatca gcgtttgcag tttcaatata gtgttcaccg ttgtttgtac
     5281 gagcgtatag tgttctcgca tttatgctct tgatggcgcc tttctgatat ctatcatcat
     5341 tgagcgatgt cttcaaatca acataatagg cgctgttctc ttgaagcttt ttggtattgt
     5401 ccgtcatgtc atagtagtcg gtgagaatct cgctcgtggt accaacctgc tgattacgat
     5461 tgtatcgacc ctgaacataa tttcccgaac tccaatccgc ctcgatagct accatacgaa
     5521 cactgtctgt cagattatca acgcggatca acgagatttc ctgcccatca gacgtcggat
     5581 accataagtt cttgtcgttt acatccatat aataacgtga atttcctgcg gcatcctctc
     5641 tggtattgcc attgaaaacg tgatctgtcg taccgttaaa gtagctattg attggatgtg
     5701 atccgtttga aaaatacact tcaaattctt catcgctttc gtcttgtttt actttcactt
     5761 taatcgatcg gcatgcaaaa aaagtaccgg caatctgcat gcttttggaa agagtatggg
     5821 gttccaagga agtagattta ataaaaaaaa tgagtttgtc ggtttgttta tcgggatcga
     5881 gttgaacggt cgtttccgat acggactgtc cgtcaaaata gaaaacgggc atatccatca
     5941 caggaagacc agtcaattct tcctgaatgg aaggagagaa attatacacg tccggatcaa
     6001 ggcctacata gtaaggtgtt gtacctagag atttagaaag ggacttgaca ttggaattgt
     6061 catacatctc gatttcaata tcctgaacaa atccgttact cttcgacaat tttattgagc
     6121 tcggcagatc ggcggatttc acagcgcctt tttttaattt ttcaataaga gtttgatcga
     6181 tccaactggg atcgcttgca agagtctggg ccatctcatt aagtgtttcg cgaatttcca
     6241 catcttccag cgatgcgttg tccatctgtt gatgatcggt acaggaagca atcatcacga
     6301 agaccataag caaaggaaat tttttcatgg gaactccatg gttgaggaac aggttgcatt
     6361 taggcgtaaa acatcctata gttacaactg aggaaggcgg tattactaaa aaattcaaag
     6421 ctccgaagca ctgaattcgg taatattgtt cttcctttta ttgttcttcc tttttttgtt
     6481 ccaggttaaa caaagcaagt taattacggc atctgccttt atttgatgaa gtcgttcacc
     6541 tccttcaaat aacaatttta agtactccaa taatgattaa aaaaaaccat tggattggat
     6601 tggattggat tggattggat tggattggat tctacttaga aatcccctca tcccatttca
     6661 tacgacgtgt aatgatacta tagcaacctt gaattgtcaa gaaaattctt gaaaacggta
     6721 atgtcgccgg tatgatgccg catccggagc gttgtgcaga tccggcttta ggtcaaaatg
     6781 acgggctcaa agtttttgaa tcgctgctta tagcgcggca aaaagcggca taatttttta
     6841 cgccatcatt ttccaaacat cataaccgaa acgaagtatc aatccgccga cgacgacgag
     6901 aaatacgtat cggataaacc gattaccttt caatacagcc attcggctgc ccaccaccga
     6961 acccgcaatc tggcaaaacg ccatcggtat ggccaacttc cagaacacaa acccgttcgt
     7021 aataaaaaat atcagcgatg agatgtcggc gacgacgttg acaattttgg ccattgcgga
     7081 tgatttcaaa aaactaaagc cgataaccga aacaaacgca aatacgagca ggctgcccgt
     7141 acccggtcca aaaaaaccgt tataaaagcc gagcacggca cacacgagta tcgctgtagc
     7201 cggtaaccgc ca
//
LOCUS       DAOQNQ010000167         7201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3945_length_7201_cov_6.150994, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000167 DAOQNQ010000000
VERSION     DAOQNQ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7201
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3945_length_7201_cov_6.150994"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3079
                     /locus_tag="PK754_10460"
     CDS             <1..3079
                     /locus_tag="PK754_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNB09970.1"
                     /translation="APDGKTFITASHDRTVRLWSRDGRLMRIFTAEEPLWSARFSPDG
                     KHIIAAGSGNLIHVWNVLGKLENIFETDQGPITDIQFAPDGQRFAFGNHDDVVLGNLD
                     GTFEKALEGHRGLVLGVAFSPDGLLIASCSEDKTVRIWNCFGDSVGIIRNFSNKVSHV
                     IFSPDGTSIFTSGFDPVIREWSLEGKLLRAFYGHRDNVYAVTISPDSRFVISGGYDRA
                     LRLWSRDGKLLRTFETMHREAISDVRFSPDGKDIISASFDKSAIIWKIDGTWVRTLGN
                     TYKPGEGGHWVQSAVFTPDGQHMIAAHWDERIVVWNTIGGIRCRVRTPSKIISSLDVA
                     PLGNFFAVGTGDGYVHIYNLDGFLQMTFNAQSPVDCIAFTSDGERLLSGGFDQRISVW
                     NLKTKMRERTFGPTKDRVNSIAVSPDGKSVVTGSNFGEVKLWDMDGTARKTFSGHGDY
                     GMIVSTRYASNGRYFASLSVNDQRSLIVRDLSGATVLDIPTAHNKNFGAVAFSPDGRY
                     IACTVDDKVQLFRFDGTLSKTFAGHTGYVNTLTFSSDSKHLLSGGDDGSIRLWHVGSL
                     ESAVYVSDDEEWVMFTPEGFFDASLGGGKFTAMVQGMNAFSMDQFASVNNRPDLILER
                     LDIGDETLRRHYEAQFLKRVRMAQEPPAFTAAPSATIDSCRIEEDKAILTTTLVSSSA
                     ALRSYQIYVNNVPLFDAPGGQLSGYHTTTCDTIHLTPGLNKIEISCVDAMNVESLRAW
                     QQVSCTKPSASELYFLGFGVSHYRDTMLSLQYADKDVKDLAGTFEKMNAGPFARVHTR
                     LFIDDSASTASLSLATNFLNKAKENDVVILFIAGHGLHDESPDATYYFLPYEADRDAL
                     TTTAIPFDAFEQLLHRTKARRKLFFMDTCESGEADEGRGAISSLNTTKTIRSRAARGF
                     KSSSGQRSYLFQKDRYIYNNLLRRSGTIVFSSSLGGEYSYERDDFQNGLFTEALIAAL
                     GGAADANYDAKVDTYELRRYLRKTVSSWSNGMQNPTVDRDNISQLISLPVLRR"
     gene            3124..5442
                     /gene="xdhB"
                     /locus_tag="PK754_10465"
     CDS             3124..5442
                     /gene="xdhB"
                     /locus_tag="PK754_10465"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HNB09971.1"
                     /translation="MHSSIPHDSAAKHVSGRSVYIDDIPEPPQMLHGHVVYSAHAHAR
                     ITHIELEDARASAGIHAVLTWRDIPGHNQMGPVVHDEPCLAEHETTCIGQAIVLIAGD
                     SPEACFQAAKKIKIEYEPLEVITDLPTAIQKNALLGPARKIRRGKPDEYLQNATHRLN
                     GSLFTGAQEHWYLETQTCLAVPGEGDEINLYSSTQHPSETQAIVSEVLGIAKHDVVVE
                     VRRMGGGFGGKETQGNHTAAWAALLARSTGRSVKIRLFRDDDQIMTGKRHPFLSHYEV
                     GFDDHGKITALKIELNADAGAATDLSFAILERAMLHSDNAYFIPHIEIIGNAWKTNLP
                     SNTAFRGFGGPQGMAVIENIVDQIARSLQKDAAEVRQINFYGTNENQTTPYGQVIEQN
                     RLPVIFEQLMNTSDYWERRRAIHLFNAENEFQKKGMALTPVKFGISFTTSFLNQAGAL
                     VHIYKDGSVLVNHGGTEMGQGLHTKIAQIAAKELGVPLTRIKVNATNTSKVPNTSATA
                     ASAGTDLNGMAVKNAIDQLKLRLMRVAADYFNERALEVRSSPDDIVFEDDTVYDMRYP
                     KRLIKFDELMPITVMKQVSLSATGYYRTPGIFFDREKGEGRPFHYFAYGMCVTEVLVD
                     TLTGEYSFLRTDILHDVGESINPAIDIGQIEGGYIQGLGWCTTEEIKHDAAGRLLTHS
                     PDTYKIPGVQDIPKDFRVALLQNAPNANTIHSSKAVAEPPFMLGLSGWLAIKDAVSAV
                     SHHRYEPEFTLPATNEAVLTAIEKLKPKIKPQ"
     gene            5452..6084
                     /gene="udk"
                     /locus_tag="PK754_10470"
     CDS             5452..6084
                     /gene="udk"
                     /locus_tag="PK754_10470"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014643843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNB09972.1"
                     /translation="MSKKHILIGVAGGTASGKTSVSKKIFETLGGHNQVVIIAQDSYY
                     KDLSQIPHQERAGHNFDHPDAFDNHLLVDHLKQALRGETIRIPNYDHKTHARTSEVTA
                     LEPHKVILLEGILILENPELRDLMDIKVYIDTDADVRLIRRLQRDIRERARTIDSVIE
                     QYEKFVRPMHLQFVEPSKRYADIIIPHGVENVVGVDILMTKIRTLLNEIQ"
     gene            6107..6601
                     /locus_tag="PK754_10475"
     CDS             6107..6601
                     /locus_tag="PK754_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09973.1"
                     /translation="MRTFLDKNRIAGFIIYPLGDLIAQIIWGEVNGVRIVTMALAGGL
                     IYAFEIPLYFGWINRTFNHWFPRTLAAIAYFNPLWIARHIFLIRASLNPPTILTWPSL
                     SVLLHTSWISGLSSFTGAIVISFAGNYIIQNMIPLERRFIASSIFSGLMAVYYAISEA
                     FLKG"
     gene            6625..7155
                     /locus_tag="PK754_10480"
     CDS             6625..7155
                     /locus_tag="PK754_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNB09974.1"
                     /translation="MALMLGAIHFSAEKHRHQRRKDPAASPYINHPIQVAELLWRVAE
                     VRDPDIIVAAILHDTIEDTQTTPDEILGLFGKTVLTMVLEVTDDPSLMKSERKQKQIA
                     TAAALSPAAKVIRLADKICNVRDVTHSPPRGWDTLRRKEYYDWTEKVVTGLRGVNPAL
                     ELFYDQVLAEGHALIP"
BASE COUNT         1872 a         1768 c         1693 g         1868 t
ORIGIN      
        1 tgctccggac ggcaaaacct tcatcaccgc ctcgcatgat cgaacggtac gcctatggag
       61 tcgcgacggg cggctgatgc gtatctttac ggctgaagaa ccgttgtggt cagcgcgctt
      121 ttcgcccgac ggaaaacata tcatcgcggc aggtagcggt aacctgatcc atgtgtggaa
      181 tgtactcgga aaattagaaa atatattcga aacggatcaa ggacctatca cggatattca
      241 atttgccccc gacggtcaac gattcgcctt tggaaatcat gacgacgttg ttttgggaaa
      301 tcttgacggc acgtttgaaa aagctttgga aggccatcgc gggttggtac ttggtgttgc
      361 cttttcaccc gacgggcttt tgatcgccag ttgcagtgag gataaaaccg ttcgtatatg
      421 gaattgtttt ggcgacagcg tcggtattat tcgtaatttt tcaaacaaag tctctcatgt
      481 gatattttct ccggacggca catctatttt tacctctggt ttcgatccgg taattcgcga
      541 atggtcattg gaaggcaagc tcctccgggc attttacggg catcgcgata atgtgtatgc
      601 cgtaaccata tcaccggaca gtcgttttgt gatttccggc ggttatgatc gtgcactccg
      661 attatggagt cgtgacggaa aacttcttcg tacgtttgaa acaatgcatc gggaagctat
      721 ttccgatgtt cggttttcac ccgacggcaa agacatcatc agcgccagtt ttgataaatc
      781 agccattatc tggaaaatag acggaacatg ggttcgtact ttaggaaata cctataaacc
      841 cggcgaaggc ggccattggg ttcaatcggc ggttttcacg ccggacggac aacacatgat
      901 tgctgcacat tgggatgaac ggatcgtcgt gtggaacacc atcggcggga ttcgctgccg
      961 tgtccgcact ccgtcaaaaa taatttcctc tctcgatgta gcgcccctcg gcaatttttt
     1021 cgccgtgggt acgggcgatg ggtatgtaca catatataat ttggacgggt ttttgcaaat
     1081 gacctttaat gcgcaatctc ccgttgattg tatcgcgttt acctcggacg gcgaacgcct
     1141 tttatcgggc ggttttgatc aacgcatatc cgtgtggaat cttaagacaa aaatgcgcga
     1201 gcgtacattc gggcccacca aagatcgtgt caattcgatc gctgtatcgc cggacggaaa
     1261 atccgttgtg acgggcagta attttggaga agtaaagctt tgggacatgg acggtacagc
     1321 acgtaaaaca ttcagcggcc atggcgatta cggcatgatc gtaagtacgc ggtacgcctc
     1381 caatggccgt tactttgctt cgctcagtgt taacgatcaa agaagtctta tcgtacgcga
     1441 tctaagcggc gctacagtac ttgacattcc tacagcacat aataaaaatt tcggcgccgt
     1501 ggcattttct cccgacggac gttacatagc ctgtactgta gatgataaag tacaactttt
     1561 tcggtttgac ggtacactat ccaaaacatt cgcagggcat acgggttacg tcaatactct
     1621 tacgttttca tccgacagca aacacttgct tagcggcggt gatgatggca gcatacggct
     1681 ttggcatgta ggcagccttg aatcggcagt ctatgtatcc gatgatgaag aatgggttat
     1741 gtttacgccc gagggttttt ttgatgcatc gctcggcggg ggtaaattta ccgctatggt
     1801 gcaaggaatg aacgccttta gcatggatca gtttgcttcc gtcaataacc ggcccgatct
     1861 tattctggaa cgattggata tcggtgacga aacgttacgc cgccattatg aggctcagtt
     1921 tttgaaacgt gtgcggatgg ctcaagaacc tcctgccttt acagctgcac ctagcgctac
     1981 aatcgacagt tgccgaatcg aggaggacaa ggctattctg accactaccc ttgtatcatc
     2041 ctctgccgca ttgcgttcgt atcaaattta tgtaaataat gtccctttgt ttgacgcacc
     2101 aggtggtcag ttatcaggct atcacacgac gacctgtgac actattcatt tgacgccggg
     2161 attgaataaa atcgaaatat cctgcgtaga tgcgatgaat gttgaatccc tgcgcgcctg
     2221 gcagcaagtc agttgcacca aaccgtcggc atccgaactc tattttcttg gctttggcgt
     2281 ttcacattac cgcgatacta tgctttcact gcaatacgcc gataaagatg tcaaggacct
     2341 tgctggtaca tttgaaaaaa tgaatgcagg gccttttgca cgtgtacata cacgactttt
     2401 tatagacgac agtgcaagca cggcatcgct ttctctcgcg actaattttc tgaataaagc
     2461 caaagaaaat gatgttgtga tcctttttat tgcaggtcac ggtctccatg atgaaagtcc
     2521 cgatgccacc tattattttc ttccgtatga agccgaccgc gatgcgctca ctacgaccgc
     2581 tattcctttt gatgctttcg aacaattgtt gcatcgcacc aaagcccgcc gcaaactgtt
     2641 ttttatggac acctgcgaat ccggagaagc cgacgaaggc cgtggcgcta tatcttcatt
     2701 aaatactaca aagaccattc gatcacgagc tgctcgcggg tttaaatctt cttcgggcca
     2761 acgctcttac ctctttcaaa aagaccgtta catctataat aatcttttac gtcgttctgg
     2821 cacaattgta ttttcctcat cactcggcgg ggaatattcg tatgaacgcg atgattttca
     2881 aaacgggctt tttacagagg ctttaatcgc cgcgcttggc ggagccgcgg atgctaatta
     2941 cgatgcgaag gttgatacgt atgaactgcg ccgttacctg cggaaaacgg tttcatcctg
     3001 gtccaacggc atgcagaacc ctaccgtaga tcgggataat attagtcaac tcatctccct
     3061 tccggttctt cgccggtagt gcaacgcgaa ttgattttac cgaaaatatt gatagattta
     3121 attatgcact cctccatccc tcatgacagc gcggccaaac atgtgtccgg ccgttcggtt
     3181 tatattgacg acatcccgga gccgccgcaa atgctgcacg gtcacgtggt gtatagtgct
     3241 catgcccacg cacgcatcac gcatatcgaa cttgaagatg cacgcgcttc cgcgggaatc
     3301 catgccgtgc tgacctggcg cgatataccc ggccacaacc aaatggggcc cgttgtacat
     3361 gatgaaccgt gcctcgcaga acacgaaacg acctgtatcg gccaggctat cgtactgatc
     3421 gcgggtgact cacccgaagc atgctttcag gcggcgaaaa aaataaaaat cgaatatgag
     3481 cctctggaag tgattaccga tttgccgacc gcgattcaaa aaaatgcact gctcggaccg
     3541 gcgcgaaaaa tccgccgcgg caaacccgat gagtacttgc aaaacgcaac gcatcggctt
     3601 aacgggtcgc tgtttaccgg agcgcaggaa cattggtatt tagaaacaca gacctgtctt
     3661 gcggtaccgg gcgaaggcga tgaaatcaat ctctacagtt ccacacaaca tccttccgaa
     3721 acgcaagcga ttgtctccga agttttaggc attgccaaac atgacgtcgt cgtcgaagtg
     3781 cggcgtatgg gcggcggttt cggcggtaaa gaaacgcaag gcaaccacac ggccgcgtgg
     3841 gcggcattgc tggcccgatc caccggacgc tctgtaaaaa ttcgactatt tcgtgatgac
     3901 gatcagatca tgacgggaaa acgtcacccg tttttatcgc attatgaagt cggatttgat
     3961 gaccacggaa aaatcacggc tctaaagatc gaactgaatg ccgacgccgg cgctgctacg
     4021 gatttatctt ttgcgatttt agaacgtgcg atgttgcata gtgacaatgc ttatttcatc
     4081 ccccatatcg agatcatcgg caacgcatgg aaaaccaatc tgccatccaa cacagcattt
     4141 cgcggattcg gcggcccaca aggtatggcc gttatcgaaa acatcgttga ccagatagcg
     4201 cgaagcctcc aaaaagatgc cgcagaagtc cgtcaaatta atttttacgg aacgaacgaa
     4261 aatcagacga cgccttacgg gcaagtgatc gaacaaaatc gtttgccggt catctttgaa
     4321 caacttatga atacttccga ttattgggag cgccgccgcg ctattcacct gtttaacgcc
     4381 gagaatgaat ttcaaaaaaa agggatggcc ctgacgccgg taaaattcgg tatttcattt
     4441 acgacttctt ttctcaatca agccggcgca ctggttcata tctacaaaga cggcagcgtg
     4501 ctcgtcaacc acggcggcac ggaaatgggt cagggactcc acacgaaaat tgctcaaatt
     4561 gcggccaaag aactcggcgt gcctctcacc cgcatcaaag tcaacgcgac caatacatct
     4621 aaagttccga atacctccgc cacagcggct tccgccggaa cggatctcaa cggcatggct
     4681 gtcaaaaatg cgattgatca attaaaatta cgattgatgc gtgtcgccgc agattatttt
     4741 aatgagcgcg ccctcgaagt gcgttcatcg ccggatgata tcgtatttga ggatgatacg
     4801 gtttatgata tgcgttatcc caagcgcctt ataaaatttg acgaactcat gccgatcacg
     4861 gttatgaaac aagtcagcct cagtgctaca ggttattatc gcacaccggg aatatttttt
     4921 gacagagaaa aaggcgaagg acgacctttt cattattttg cttacggtat gtgcgttact
     4981 gaagtgttgg ttgacacgct caccggcgag tattcatttt tgcgcacgga tatattgcac
     5041 gacgtcggcg aatccatcaa tcctgcgatc gacataggcc agatcgaagg cggttatatt
     5101 caaggcctcg gttggtgcac taccgaagaa atcaaacacg atgcggcggg tcgtttactc
     5161 acccattctc ccgatacgta taaaattccc ggcgtgcagg atattcccaa agattttcgc
     5221 gttgcgcttt tacaaaatgc gcccaacgcc aatacgatac attccagcaa agcggtagcc
     5281 gaaccgcctt ttatgctagg tctttcaggc tggctcgcga tcaaagatgc ggtgtcggcc
     5341 gtaagccatc atcgctacga accggagttt acattaccgg ctacgaatga agccgtatta
     5401 acagccatcg aaaaattgaa acccaaaata aaacctcagt aacgtacacc tatgtccaaa
     5461 aaacacatct tgatcggcgt cgccggagga actgcttccg ggaaaacatc cgtatccaaa
     5521 aaaatctttg aaacccttgg cggtcataat caagtagtca tcatcgctca ggattcgtat
     5581 tacaaagatt tatcgcaaat cccgcatcag gaacgcgccg gtcataattt tgatcatccc
     5641 gacgctttcg ataatcattt gttggttgat catcttaaac aagcgctgcg cggcgaaacc
     5701 attcgcattc ccaactacga ccataaaact catgcacgca cttcggaagt gactgctctc
     5761 gaaccgcaca aagtgatctt actggaaggt attttgattt tagaaaatcc tgaattacgc
     5821 gatctgatgg atatcaaagt ctatattgat accgacgcgg atgtgcgact gattcgccgt
     5881 ttacagagag atatacgtga gcgtgcacgt acgattgatt ccgtcattga gcagtatgaa
     5941 aaatttgtgc gccccatgca cttacaattt gtcgaacctt ccaaacgtta tgccgatatt
     6001 atcatcccgc acggtgtaga aaatgtcgtc ggtgtggata ttctgatgac taaaatccgc
     6061 acattgctaa acgaaatcca ataagctacg gcgaccaccc tgaacaatgc gcacatttct
     6121 cgataagaac cgtatagccg ggtttatcat atatcctttg ggtgatctga tagcacagat
     6181 catatggggt gaggttaacg gagtacgtat tgtgacgatg gcgctggccg gcggtttgat
     6241 ctacgcgttt gagatcccat tatatttcgg atggatcaac cgaacattca accattggtt
     6301 cccacgcacg ctcgccgcta tcgcatattt taatccactt tggatcgccc ggcatatttt
     6361 tcttattcga gcgagcctca atcccccaac cattctgact tggccttcgt tatcggtttt
     6421 actacacact tcctggataa gcggcttatc ttcttttacc ggcgcgatcg tgatttcttt
     6481 tgccggtaac tacatcatac aaaacatgat tcctctcgaa cggcgtttta ttgcgagcag
     6541 catttttagc ggactcatgg ccgtttatta cgcgatatca gaagcgtttc tgaaaggtta
     6601 attatgatct atgctgagac ggacatggcg ctgatgttgg gtgcgattca tttttccgct
     6661 gaaaaacacc ggcaccagcg acgcaaagat cctgctgcgt cgccgtatat caatcatccg
     6721 attcaggttg cagagttact ttggcgtgta gctgaagtac gcgatcccga tattatcgtg
     6781 gccgcaattt tacatgatac catagaggat acacaaacca cacccgatga aatcctcgga
     6841 ttattcggaa aaactgttct tactatggta ttggaagtaa ccgacgatcc gtcgttaatg
     6901 aagagcgaac gtaaacaaaa gcagattgct accgctgccg cattgagccc tgccgctaag
     6961 gtcatacgcc ttgccgacaa gatttgcaac gtacgcgatg tgacgcattc gccgccacga
     7021 ggatgggata cgctgcggcg taaagaatat tatgattgga ctgaaaaagt ggtgacggga
     7081 ttgcgaggtg tcaatcctgc gctagaattg ttctacgatc aggtattagc cgaaggtcac
     7141 gcattaattc cgtagctaaa tgcaactcat tctttagatc gaatatgctc tacgatcgtt
     7201 t
//
LOCUS       DAOQNQ010000168         7170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3969_length_7170_cov_5.417428, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000168 DAOQNQ010000000
VERSION     DAOQNQ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7170
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3969_length_7170_cov_5.417428"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PK754_10485"
     CDS             <1..782
                     /locus_tag="PK754_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNB09975.1"
                     /translation="YGSPRKNKPFVAVNCSAFSRELLESELFGYKAGAFTGAHRNKRG
                     LFEEADQGTLFLDEIGELSAEAQAKLLRVIELGEFIKTGDTRSTRVNVRIIAATHRDL
                     AAESAAGRFRQDLFYRISVFQVKLPALRDRSEDIPAFVEYFVRRFSEKLNKRLRFADK
                     GDWLALQTYDWPGNIRELRNVIERAVILSDGEELYSAQLLRFIYDNPSSDSNTMDLKH
                     VEREHIRKILQKTGGHKADAARLLGIGLTTLYRKIEEYALN"
     gene            1008..2918
                     /locus_tag="PK754_10490"
     CDS             1008..2918
                     /locus_tag="PK754_10490"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNB09976.1"
                     /translation="MQSGNRGFKAFALLTLSTVGVVYGDIGTSPLYALREVFLGHHPI
                     VTSVENVLGALSLFFWSLTIVVALKYHILVLRIDNQGEGGVFALLAIIKQAKLQGRLT
                     MIVSGGLILGACLLYADGLITPAISVLSALEGIEVATPALSSWVIPLTIAILIGLFLV
                     QKHGTTGVGRFFGPIMVVWFVSLIVIAWPYVWSKPEVFNAVNPMYAIHFLAQQGIGSS
                     MFILGSVFLCVTGGEALYADLGHFGKHAIDTAWFILVYPALVFNYFGQGAYLLSHDVA
                     VGSNLFYLLPSETFLIPMVILATMATIIASQAMISGAFSLTQQAIGLGFFPRLKIVHT
                     SASVHGQIYMPAINWLLCAGCVALVLTFKSSGALASAYGITVTATMTITTMGFFLVTT
                     RIRRWSIFWVAPLCAGLFAIDLSFFVSNAIKFLDGGFVPVTLGFVICGVMLLWSWGKK
                     KLSHVYAPDGMTVADLMALKDVEWKPVIPRRIHFFSPSPVTSESSLVPLTLQIFVSRI
                     HVLPRELSFVTVKIHNQPYVEGPRVEHYTLRNDVRAVVIHYGYMEDPDIVTILERHEI
                     KGNIVVGDHEIFSEHEHGLHYLQVRWFRLLLRLSLPSYRYFGVQRMSRIMKEVIPVEI
                     GKKGAVLLDVDY"
     gene            2988..4772
                     /locus_tag="PK754_10495"
     CDS             2988..4772
                     /locus_tag="PK754_10495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB09977.1"
                     /translation="MLTLKQKLFAGLLLLWSVMLLIGGLGTYAIIRLSDESSLIIKDN
                     YKSLGYAQNMIGALDDAYASNDAAVMMDTLALMKAYARFDSNFSHELNNITEPGEREL
                     VMQLYGSFNEFKSNPKNTRERYNCYLRMRTSLRAVFDVNMNAILRKHDYAQASARTIM
                     IAMAAVGIPCLILTLIFVLRFPGYIAAPIRTLSEGIRSIAEKNYERRVNITTKDEFGE
                     VAASFNRMASRLEEYDNSNWARILFEKTRIETIIRNMHDPILVLDERGFVLFTNPAFS
                     EVVAVPEKNLVGQYASTAADQNIHLHSILNPAMNDSTDDTIRLRSPNKDLIFRREQFS
                     VIRTDDDTHTSQALGRVIILKNVTQFHELDMAKTNFMATISHELKTPLSSIDLSVKLL
                     QDGRTGMLSDEQKKLVRAIRQENSRLMKLVSELLDMTQLESGQLRLTIGRVSVMEIIL
                     LATDALEFQLHQKSIRITTDIPHDVPFVSADLDKTVWVMVNLLTNAVRYTAEGGTIRI
                     AVRKNDQTVEFSVEDYGEGIDPKHHARIFERYIRLENPSTGDGGGTGLGLSTVRDFIL
                     AQRGTIQMESIPGKGSRFYFTLPVFAEG"
     gene            complement(4749..5975)
                     /gene="hutI"
                     /locus_tag="PK754_10500"
     CDS             complement(4749..5975)
                     /gene="hutI"
                     /locus_tag="PK754_10500"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNB09978.1"
                     /translation="MTLFIQNIKEVVFFEKDLFRTANAVDIRIENGMIADITPTGTTS
                     APAHATILDGASYVALPGLIDSHTHTIFGGSREDEFEMKLRGATYQDIAAKGGGIKST
                     MHKTRAASKEELLQLGIRRCQSALALGITTMEMKSGYGLSVEAEIKILETIRDVRQHT
                     PIEITSTFLGAHTIPPEYSQQREAYIRLVCETMIPMVTEQRLAEFCDAFCETNVFTPD
                     ETRHIFETAKKYGMKLKLHADQLTNTGGAELCAEMGAISADHLETTSDKGLAALAGSN
                     VVAGLLPGCSFYLRMQYPPARKMIDMGIPVALATDFNPGSCPTQNLQLIMSIACTQMR
                     MTPDEAIKGVTLHAARALDKAHIGNIAVGMQADIVLFDVPGYRYIPYNFGQNHVAHVI
                     KKGRVVHSASSLGEDR"
     gene            6146..7108
                     /locus_tag="PK754_10505"
     CDS             6146..7108
                     /locus_tag="PK754_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HNB09979.1"
                     /translation="MIQIQDILNAYKTVQPIVHKTPLMQSHALDEKTGNAVFFKCENF
                     QKVGAFKIRGAYNKIASLSAEQKAKGVVAFSSGNHAQGVALASRLLGTTSKIVMPDNS
                     PKSKMEATRAYGAEVIVYDFLKEDREAIGKRISESEGRTLVPPFNDEYIMAGQGTVSV
                     ELVEQISDLDYAFIPCGGGGLLSGNAVALKHFYPHIKVVGVETDTANDAYQSFNKGEI
                     VKIAPPPTIADGMRTLALGDKTFPVIRRYVDAMLTVSDHAVIEAMYWIYSRLKIVVEP
                     TAAVPFAAVMQNTLQLRGKKIAVILSGGNIDLDDFFELLKRQAA"
BASE COUNT         1832 a         1552 c         1799 g         1987 t
ORIGIN      
        1 actacggcag cccgcgtaaa aacaaacctt ttgtcgccgt gaattgcagc gcgtttagcc
       61 gagagctttt ggaatcagaa ctattcgggt ataaagccgg tgcatttacg ggcgcacata
      121 gaaacaaacg gggcctgttt gaagaagcgg atcaaggcac tttgttttta gatgaaatag
      181 gcgaactatc cgcggaggct caggcgaagt tattgcgtgt catcgaattg ggggagttta
      241 ttaaaaccgg cgatacccga tcgacgcgag taaatgtacg cattatcgca gcaacgcatc
      301 gtgatctagc ggccgaatcc gctgcagggc gtttccggca ggatcttttt tatcgcatct
      361 cagtgtttca agtcaaatta ccggccttgc gcgaccgatc cgaagatata ccggcttttg
      421 tagaatattt cgtccgccgt ttttccgaaa aactcaacaa acggttgcgt tttgcagata
      481 aaggcgattg gcttgcactg caaacatacg actggcccgg caatattcgt gaactgcgta
      541 atgtgatcga aagagccgtg atattatccg acggagaaga attgtatagt gcgcagttgt
      601 taaggtttat ttatgataat ccgtcttcgg attcgaatac gatggatctg aagcatgtcg
      661 aacgggagca cattcgaaaa atattacaaa aaaccggtgg ccacaaagcc gatgcggcgc
      721 gtcttttggg tatcgggttg acgacactgt accggaaaat agaagaatac gctttgaatt
      781 aaagtgcgaa tttttttcaa aatgaaaaaa agccttccat aatggaaggc tttttttttg
      841 ttgcatcaat atctcggatg tgaactctat aatacacaat gtttataaac ccggcatctt
      901 tggcataagg attggttgta tatcatcgct gtatcggcca tacgctatca ccttggaata
      961 gtgtctttga tgggtggcca agtcacaaca taagatgagg agatgttatg cagtccggaa
     1021 atagaggatt taaggcattt gcattattga cattatcaac ggtcggtgta gtatatggag
     1081 atatcggtac atcacccttg tatgctttgc gtgaagtttt tttggggcac catcctatcg
     1141 taacatctgt tgaaaatgta ttaggcgcgc tatctttatt tttctggagc ctgactattg
     1201 ttgtagcatt aaaatatcat atactcgtac tgcgtataga taatcagggc gaaggcggcg
     1261 tatttgcttt actcgctatt atcaaacaag ccaaattaca aggccggttg actatgatcg
     1321 tgtcgggcgg attgatcctc ggtgcatgtt tattatatgc tgacggactt atcacaccgg
     1381 ctatttccgt tttgtcggcg ttggaaggta tcgaagtggc cacgccggct ttgagttcat
     1441 gggtcattcc gctgactatc gcaatcctca tcggattatt tctcgttcaa aaacacggca
     1501 cgaccggagt cgggcgattt tttggtccca taatggtggt gtggtttgta tcattgatcg
     1561 tcatcgcctg gccgtatgta tggtccaaac ccgaagtatt caacgccgtg aacccgatgt
     1621 atgcgataca ttttttagcg caacaaggta tcggcagttc catgttcatt ctgggttctg
     1681 tttttttgtg tgtcaccggc ggagaggcgt tgtacgcgga ccttggtcac ttcggaaaac
     1741 atgcgattga taccgcgtgg tttattttgg tatatccggc gttggtgttc aattattttg
     1801 gtcaaggcgc ttatttactt agccatgatg ttgccgtcgg atccaatctt ttttatttat
     1861 taccctcgga aacatttttg ataccgatgg taatacttgc tacgatggct acgattatag
     1921 ccagtcaggc tatgatttcg ggcgcctttt ccctcacgca acaagccatc ggtctcggat
     1981 tctttccgcg gttaaaaata gttcacacca gcgcttccgt gcatggtcag atatatatgc
     2041 cggcgatcaa ttggcttttg tgtgcaggat gtgtggcgct ggtgttgaca ttcaaaagct
     2101 ccggcgcatt ggcttcggct tacggtatca cggtgacggc caccatgacg atcacgacta
     2161 tgggtttttt tctcgtaact acgcgcattc gccggtggtc tatattttgg gtggcgccgc
     2221 tgtgtgcggg attatttgcg atagacctca gtttcttcgt atccaatgcg atcaaatttc
     2281 tcgacggcgg atttgttccc gttacgttag gttttgtgat atgtggcgta atgttgctct
     2341 ggtcgtgggg taagaagaaa ctcagccatg tgtatgcgcc ggatggcatg accgtcgccg
     2401 atctgatggc actcaaagat gtcgaatgga aaccggtcat tccgcgccgg attcattttt
     2461 tctcgccctc accggtcaca tcggaaagca gccttgtccc gcttacgctt caaatttttg
     2521 tttcacgcat ccacgtatta ccgcgcgaac tttcgtttgt gaccgtaaaa attcataatc
     2581 aaccgtacgt ggagggccct cgtgttgaac actatacgtt gcggaatgac gtgcgtgccg
     2641 tggttataca ctacggttat atggaagatc cggatatcgt aacgatcctg gaacggcatg
     2701 agatcaaagg taatatagtc gtcggcgatc atgaaatatt ttcagaacat gaacacggat
     2761 tgcattattt acaagtgcga tggttccgct tgctgctgcg gctatcatta ccctcctacc
     2821 gttatttcgg ggttcaaaga atgtcacgga tcatgaagga agtgattccc gtcgaaatcg
     2881 gaaaaaaagg ggccgtactt ctggatgtgg attattgata tattttcact gttttacgaa
     2941 agtccggcgg cgtactttta acaacatact atgaaagtgg atgtccattg ttgacgttga
     3001 aacaaaaatt atttgctgga ttacttttgc tttggagcgt gatgttactc atcggaggat
     3061 tgggtacgta tgcgattatc aggctttcgg atgaatcttc gttgatcata aaagataact
     3121 ataaatcact gggttatgca caaaacatga taggtgcatt ggatgacgct tatgcgtcga
     3181 acgatgcggc ggttatgatg gatacgctgg ctctgatgaa agcgtatgcg cggtttgaca
     3241 gtaatttttc gcacgaactc aacaatatca ccgagcccgg tgaacgcgag ttggtgatgc
     3301 aattatacgg atcgtttaac gaatttaaat cgaaccccaa aaatacccga gaacgttata
     3361 attgctatct ccgtatgcgg actagtctgc gtgctgtttt tgacgttaat atgaatgcca
     3421 tactcaggaa gcatgattat gcccaagcgt cggcgcgaac gatcatgata gcgatggccg
     3481 cggttggtat accttgcctg attctgacgt tgatttttgt tttgcgtttt cccggttata
     3541 tcgcggcgcc gatacgtacg ttgtccgaag gtatacgcag tattgctgaa aaaaattatg
     3601 aacgccgagt taacataacg accaaggatg aattcggcga agtggccgct tcgtttaacc
     3661 gtatggcgtc acgtttggaa gagtatgata acagtaactg ggcacgtata ctttttgaaa
     3721 aaacgcgcat cgaaacgatc attcgcaata tgcatgatcc gatattggtg ctggatgaga
     3781 gaggatttgt acttttcacc aatccggctt tttcagaagt tgtcgccgtg ccggaaaaaa
     3841 atctggtcgg ccagtatgct tcgacggccg ccgatcaaaa tattcatttg cattcaatac
     3901 tcaatccggc gatgaacgat tcgaccgatg acacgatcag gctacgaagt ccgaataaag
     3961 atcttatttt ccggcgtgag caattctctg tgattcggac ggatgacgac acgcacacca
     4021 gccaggcatt ggggcgagtt attattttaa aaaatgtcac gcagtttcat gaactggata
     4081 tggccaaaac caattttatg gcgacgattt cgcacgaatt gaaaacgcct ttgtcttcca
     4141 ttgatctgag cgtcaaatta ttacaggatg ggcggaccgg catgctgagt gacgaacaaa
     4201 agaaactcgt tcgcgcgatt cgtcaggaaa attcgcgcct gatgaaatta gtgagcgagc
     4261 tgctggatat gacccaattg gaaagcgggc aacttcgatt gactatcggg cgtgtgtcgg
     4321 tcatggaaat tatattattg gcaacggacg ccttagaatt tcagttgcat caaaagtcta
     4381 ttcgcataac gaccgatatt cctcatgacg taccgtttgt aagcgcggat ctggataaaa
     4441 cagtatgggt catggtcaat cttctgacca atgccgtacg atacaccgcg gagggcggga
     4501 caatccgtat cgctgtcaga aaaaatgatc agaccgttga atttagtgtg gaagattatg
     4561 gcgaaggcat tgatcccaaa catcatgccc gcatatttga acggtacata cgtttagaaa
     4621 atccttcgac cggcgatggc ggaggaacgg gtttgggttt atccacggtg agagatttta
     4681 ttttggcaca gcgcggcacg attcaaatgg aaagtatacc cggcaaaggc agtcgttttt
     4741 actttacatt acctgtcttc gccgagggat gaagcggagt gcaccacgcg gccttttttg
     4801 atgacgtgcg cgacatgatt ttgtccgaaa ttataaggga tgtacctata ccccggcaca
     4861 tcaaacaaaa cgatatccgc ttgcataccc acggcgatat ttccgatatg agctttatcc
     4921 agagcacgcg cggcatgcag tgtcacacct ttgatcgctt cgtccggcgt catgcgcatt
     4981 tgggtgcagg cgatggacat gatgagctgt agattttgcg tcggacaaga acccggatta
     5041 aaatccgtag ctaaagcgac cgggatgccc atgtcgatca ttttacgagc cggcggatat
     5101 tgcatacgca ggtaaaatga acagccgggc aataaaccgg cgacaacatt ggatccggcc
     5161 agtgccgcaa gccctttatc ggaagtggtt tcaagatgat cggcggaaat agcgcccatc
     5221 tccgcgcata attcggcacc tccggtattg gtcaattgat ccgcatgcaa ctttaacttc
     5281 atcccgtatt ttttggccgt ttcaaaaatg tgacgcgttt catccggtgt aaatacgttg
     5341 gtctcgcaaa acgcatcaca aaattcagcc agtcgttgtt ccgttaccat gggaatcatg
     5401 gtctcgcaaa ccaaacgaat atatgcttcg cgttgctggc tgtactccgg aggaatggtg
     5461 tgcgcgccga ggaaagtgga ggtaatttca attggtgtat gctgccgcac atcgcgaatg
     5521 gtttcgagta ttttgatttc cgcttcgacc gacaaaccgt aaccgctttt catttccatc
     5581 gtggtgatgc cgagtgccaa tgccgactga caacgtcgga taccgagttg cagcaattcc
     5641 tctttggatg ccgcgcgggt tttgtgcatt gtacttttga tgccgccgcc tttagccgcg
     5701 atgtcttgat aggtggcgcc gcgcagtttc atttcaaatt catcttcgcg ggaaccgccg
     5761 aaaatcgtat gggtgtggga atcaatcaat cccggaagcg cgacatacga tgcaccgtct
     5821 aaaatcgttg catgggccgg cgctgaagtg gtcccggtgg gagtaatgtc tgcgatcatt
     5881 ccgttttcga tacgtatgtc taccgcgttg gctgtgcgga acaaatcttt ttcaaaaaaa
     5941 acgacttctt tgatgttttg gataaaaagc gtcatgggtg tatttttttg caagtgaaat
     6001 aatcgtgcaa tggtaataaa aacacaccgc aaaagaaaca gtgatgtata accaaaaaaa
     6061 cagaggacgg tatgctgtcg aaggctttga aacagcatga aacaaggttg attttaagcg
     6121 tcaatttcaa tatcattttc ggcttatgat acaaatccaa gatatactca atgcctataa
     6181 aacggttcag ccgattgtac ataaaactcc gctgatgcag tcccatgcgc tggatgaaaa
     6241 aacggggaac gcggtttttt ttaagtgcga aaattttcaa aaagtgggcg ctttcaaaat
     6301 acgcggggcg tataataaaa tcgcatcgct ttcagcggaa caaaaagcca aaggcgtagt
     6361 agctttttcc tctggtaatc atgcgcaagg cgtggccctg gcgtcgcgct tgcttggtac
     6421 gacgtccaaa attgtcatgc cggacaattc gccgaaatct aaaatggaag cgacccgcgc
     6481 gtatggtgct gaggtgattg tttacgattt tctcaaggaa gatcgtgaag cgatcggtaa
     6541 acggatttcc gaatcagagg gacgtacgtt ggtgccgccg tttaacgatg aatacatcat
     6601 ggccggtcag ggtacggtga gcgttgaatt ggtggagcaa atatcggatt tggactatgc
     6661 ctttattcct tgcggtggcg gtgggctttt atcgggtaat gccgtcgccc tcaaacattt
     6721 ttatccgcac atcaaagtgg tcggcgtaga gacagacacc gctaatgatg cgtaccaatc
     6781 gtttaacaaa ggcgaaattg taaaaatagc gccgccgccg acgatcgcgg acggcatgcg
     6841 aaccctcgct ctcggcgaca aaacctttcc tgtgatccgg cgttatgtgg atgcgatgct
     6901 cactgtgtcg gatcacgccg tgattgaggc gatgtattgg atttattcac gactcaagat
     6961 cgttgtcgag ccgaccgccg cggtaccttt tgctgcggtc atgcaaaata cattgcaatt
     7021 gcgcggaaaa aaaatcgccg tgatactttc cggcggaaat atagacttgg atgatttttt
     7081 tgaacttctc aaacgacagg ccgcttagat catgaactta gcaacgctcc ggcaaaaatt
     7141 acttgattcc tctcgcccta agatactggt
//
LOCUS       DAOQNQ010000169         7166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_3971_length_7166_cov_6.645760, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000169 DAOQNQ010000000
VERSION     DAOQNQ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7166
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3971_length_7166_cov_6.645760"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PK754_10510"
     CDS             <1..457
                     /locus_tag="PK754_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNB09980.1"
                     /translation="HVLYEDYLIHEFLPYLRNKTGTDYLILLGCSFGGYHAVNFAMRH
                     PEMVNRAIGLSGSYTIQGFLGGYYDDLCYYNNPAHYVQHLSDPYYIDRYNTHTEITLV
                     TSDLDPCRERNEYFHQILSEKGIRHNYFFWDGGIGHDWPYWQRMIGHYV"
     gene            479..1147
                     /locus_tag="PK754_10515"
     CDS             479..1147
                     /locus_tag="PK754_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09981.1"
                     /translation="MIGIRLLLFISVLFFFYFLARQPDTAETMRCDASFYGHGRVPVK
                     LRRPVNDSIPATPIRVAQFFRWTLSQDALIQHMDVLPESEPVTGFEDRAVCIEGYAVA
                     IRYDHKDGLYQKDNALYLEMTDSPRWDTPHLIVRIPPGEAYCDARTTLWNIAHWDASA
                     AKEKISAGLRLMRRPTRVRVTGFLYINPETKKKYDRHGISYQTKGIPDGIQRKRTPPQ
                     TQGV"
     gene            1195..2679
                     /gene="glgA"
                     /locus_tag="PK754_10520"
     CDS             1195..2679
                     /gene="glgA"
                     /locus_tag="PK754_10520"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HNB09982.1"
                     /translation="MKTPLKIALASSEVAPFAKTGGLADVSGALPKYLTRLGCDVKVF
                     MPKYVSVDDSKWDLHYCADIGEILIRMGRVTRSAHVLKSVIPGSEAEIYFIECPHYYH
                     RRHIYTMDRDEDERFILFCKAVIEVMQRIQWAPDVVHCNDWQTGLIPIYLKDNYNWDR
                     MFDRTACLYSIHNIAYQGRFSKDTLYRAEMRPDQFYPGGPMEFHDTFSFMKTGILSSE
                     IVTTVSHTYAKEILTYEYGNGMNDVLWPRQNDLFGVLNGIDYDEWNPEKDKYIPHPFS
                     QSDLSGKEKNKKFLLEQTHLTYDPAKPVIGIVSRLVGQKGFDLIEKALPELMLLDAQW
                     VILGSGEDRYEDMFENLHHALPYKVWTYIGYSNELAHRIEAGADIFLMPSHFEPCGLN
                     QMYSQRYGTIPVVRKTGGLADTVWDWHELHSAGRGIGNGFTFTDASPYALFTTLLRAL
                     ETYKDKTVWRKIQQNAMAMDHSWDASAQRYIELYKMAIQKRRAE"
     gene            2857..4668
                     /locus_tag="PK754_10525"
     CDS             2857..4668
                     /locus_tag="PK754_10525"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNB09983.1"
                     /translation="MRLKIKYLDGIRLKRSVIAGCNHVINKKEYLNNINVFPVPDGDT
                     GTNMASTLKNIAETVEKEELHEIDSMAIVLADSALMGARGNSGVIMAQFFQGMNEGLQ
                     GSAKCTTKKFSDAVQKATKSAWSAISNPVEGTILSVIRDWGKSVEKNAEKTDDFSELF
                     RESLADAKQSLANTPKQMAALAKAGVVDAGAQGFVTMLEGVLDFIENGRISDIKRQGL
                     SETEDEAMEAHIDEHAFEEITYQFCTECLLVGENMDREKIRESLNKFGDSLIVAGSTK
                     KVKIHLHSNTPEDVFEVLADHGHVISTKIDDMKQQFLNAHSENVQETAIAIDSCCDLP
                     LEYIRRNHIHVIPLNVHFGEQIYIDRLTLTPKKFYEKLTSSPDHPKSSQPTPAKFKES
                     YERLLQIYKNVVSIHLSSKLSGTYQGALSMARQVAQDKIFCFDSMSASVGIGVMVREI
                     EPMIRENAPAEKIMQHLEYCRNNMKVFISVQTMDYLIKGGRVSKARGAIAKLLNIRPV
                     LTVQDGAVVPIAKAFGHSNALRKVMDLVKATTKNRKKPRFGIAHANSIGIADWYATQL
                     SRYYEIDRDDILITDVSTVIATHAGPGAAAIAVLSDP"
     gene            4674..5192
                     /locus_tag="PK754_10530"
     CDS             4674..5192
                     /locus_tag="PK754_10530"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HNB09984.1"
                     /translation="MTSRVNITMPRGPYEKWIEVICISVVVASFVLVMGHIQELPESI
                     AIHFDWSGYPDNWGSKHTLWLLPALSALVIYMPLTVFSFLQQGHTKPDDISSTLIHRN
                     FKIARISLALIKLNVLFLMWWLTYIVIQSALSGRIDHNPVGVLSIFLLILTLIIGISI
                     YLVKAKRSKPSA"
     gene            5185..5901
                     /locus_tag="PK754_10535"
     CDS             5185..5901
                     /locus_tag="PK754_10535"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNB09985.1"
                     /translation="MHNWIIIFLLLHVIPVSSQTMSDPGLKKYIGLWEREQTDSLKTL
                     IAEMEKKFPKAPETVFLKNTYETDGAIAYAVYLRFVRDYPKNIFAPEAYHRMIQYDAA
                     AGRYALAQDNLIKLRTDYPGSSCIKSAETLFPATKPDEATQAIMTAPSDTARPTEINA
                     GKKFRLQVGAFSDQKNAQELKQKLTAKGYAPIEFSEKTVNGKTLILVWVGAYTTHDDA
                     KKAGDTIKSKEQLNYSIVEK"
     gene            5891..6460
                     /gene="thpR"
                     /locus_tag="PK754_10540"
     CDS             5891..6460
                     /gene="thpR"
                     /locus_tag="PK754_10540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNB09986.1"
                     /translation="MKNETIRAFIALDFPNEAFSFFSEQERRLKPLFNDARIAWVNPT
                     NIHITLAFLGEINRDAIPKITRCLSDLDEAGPFYLAATHLGFFSTKGNPKVLWIGFKK
                     TSSIESLQNKICIRLNALGLTTEEKKIIPHATLARLRDWPSARLAQQNVIHQFIGETE
                     WVMPTLTLYESVLQRQGAQYKPLFQIKFT"
     gene            6457..7146
                     /locus_tag="PK754_10545"
     CDS             6457..7146
                     /locus_tag="PK754_10545"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNB09987.1"
                     /translation="MKIASIQQKKVKRNQSATVCVLTEEGDSFELLYETLVKFGIYDG
                     SDLDEKKWNRIQLWDSTRRATTDALRWLSLRPRSREEISNRLRREGYNDETITSVLEK
                     LSEEKYIDDLDFAMRFAQSRLRKKAMGERALAYELNKKGIAPDLIRQVIQKFVPENDL
                     PSVLRLAEKKYATIRESDQRKKQIKLSAFLQQRGFDTDTIRKVVETIASHEKIISRDQ
                     NPLIPSNFKKH"
BASE COUNT         2047 a         1688 c         1600 g         1831 t
ORIGIN      
        1 ccatgtttta tacgaagatt atctgatcca tgaattttta ccctatttgc gtaataaaac
       61 cgggacggat tacctgattc ttttaggttg tagtttcggc ggttatcatg cggtcaattt
      121 tgcaatgcgt cacccggaaa tggtcaaccg ggccatcggc ttgagcggtt cgtacacgat
      181 tcaaggcttt ctgggcggct actacgacga cttgtgttat tacaacaatc ccgcacatta
      241 cgtgcaacat ctctccgacc cctattatat tgaccgttat aatacacaca cggaaatcac
      301 gcttgtaacc agtgatctgg atccttgccg ggagcgcaac gaatattttc atcagattct
      361 ctcggaaaaa ggaattcgtc acaattattt cttttgggac ggaggcatcg gtcatgattg
      421 gccttactgg caacgcatga tcgggcatta cgtttaaatc gctttagcct acattattat
      481 gatcggcata cgtttactac tatttatatc cgttcttttc tttttttatt ttcttgcgcg
      541 ccaacccgat acggccgaaa ccatgcgttg cgacgcgtcg ttttacggtc acggccgggt
      601 accggtcaaa ttgcgcaggc cggtaaacga ttcgatccct gcaaccccga ttcgcgtagc
      661 acagtttttc aggtggacgc tatcgcaaga tgcgttgatc caacatatgg acgtcttacc
      721 tgaatcggaa cccgttacag gatttgaaga ccgtgccgtc tgcattgaag gttacgccgt
      781 cgctatacgc tacgatcata aagacggtct gtatcaaaaa gacaatgcgc tgtatttgga
      841 aatgaccgat tcaccgcgtt gggatacgcc gcatctgatc gtacggatac cgccgggcga
      901 ggcttattgc gatgcgcgta caacattgtg gaacatcgca cattgggatg cttccgcggc
      961 caaagaaaaa atttcggccg gtctgcgttt gatgcgcaga cccacacgcg tacgtgtgac
     1021 cggttttttg tatatcaatc cggaaaccaa aaaaaaatac gaccggcacg gcatcagtta
     1081 tcaaaccaaa ggtattcctg acggcataca acgaaaaaga acaccgccac aaacgcaagg
     1141 cgtttaggaa attcagccgg cttttcgggt tgaattgata aactaactat gcgtatgaaa
     1201 acaccactca aaattgcttt ggcttcaagt gaagtcgctc cgttcgccaa aaccggcggt
     1261 ctggccgacg tatcgggcgc cttgcccaaa tacctgacgc ggttgggatg cgacgtcaaa
     1321 gtgtttatgc ccaaatatgt ttccgtggat gacagtaaat gggatttaca ttactgtgcc
     1381 gatatcggcg aaatcctcat tcgtatgggc cgtgttacac ggagcgccca tgtacttaag
     1441 tccgttattc ccggctccga agccgagatt tattttatcg aatgcccgca ctattatcat
     1501 cgccgccata tttatacgat ggatcgcgac gaagatgagc ggtttattct tttttgtaaa
     1561 gccgtcatcg aagtgatgca gcgtatccaa tgggcgccgg atgtcgtgca ctgcaatgat
     1621 tggcagacgg ggttaattcc gatctacctc aaagataatt acaattggga tcggatgttt
     1681 gatcgtaccg cgtgtttgta ctccatacac aatatcgctt atcaagggcg cttctccaaa
     1741 gatacgctgt atcgcgccga aatgcgcccg gatcagttct atcccggcgg cccgatggag
     1801 tttcatgaca cgtttagttt tatgaaaacc ggtattcttt catccgaaat cgttacgacc
     1861 gtcagccata cgtatgccaa agaaattctg acctacgaat acggtaacgg catgaacgat
     1921 gtgctgtggc cgcggcaaaa cgatcttttc ggcgtattaa acggcattga ctacgatgaa
     1981 tggaaccctg aaaaagacaa atacatcccc catccttttt cacagtccga tctttccggt
     2041 aaagaaaaaa ataaaaaatt tcttttggag caaacgcatc tgacgtatga cccggccaaa
     2101 ccggtgatcg gcatcgtttc gcgtttagtc ggacaaaaag gatttgatct gatcgaaaaa
     2161 gcattgccgg agctgatgct gcttgatgcg caatgggtca tactcggtag cggtgaagac
     2221 cgttatgaag atatgttcga aaacctgcat cacgctttac cttataaagt gtggacctat
     2281 atcggctata gtaacgaact cgcgcatcgt atcgaagccg gggcggatat ttttctgatg
     2341 ccttcgcact ttgaaccttg cgggctcaat caaatgtaca gtcagcggta cggtacgata
     2401 ccggtggtgc gaaaaaccgg cggacttgcc gatacggttt gggattggca tgaactgcat
     2461 tccgcaggac gcggcatcgg gaacggtttt acttttacgg atgcgtcacc gtatgcttta
     2521 tttacgacct tattgcgtgc actcgaaacc tacaaagaca aaaccgtgtg gcgaaaaatc
     2581 cagcagaacg ccatggcaat ggaccattcg tgggatgcat cggcccaaag gtatatcgaa
     2641 ctctataaaa tggcgattca aaaacgccgc gccgaataga agaacaaata aggattggat
     2701 agtaagccta aaatcggttt gtactgtaga cttatttaca attgcttgac tttcattgac
     2761 ctttaatcta attttttgac tgcacttcgt ttaggaacgc ctcaatccct tcaaatcagc
     2821 gttccgcctt gttcataccg actacaggag gatattttga gactcaaaat caagtattta
     2881 gacggcattc gcctcaaacg ctccgtgatc gccggatgca atcacgttat caacaaaaag
     2941 gaatatctca ataacatcaa tgtgtttccg gtcccggacg gcgatacggg taccaatatg
     3001 gcatccacat tgaaaaacat cgccgagacg gtcgaaaaag aagaattgca tgagattgat
     3061 tcgatggcga tcgtgctggc cgattcggct ctgatgggtg cccgcggcaa tagcggtgtg
     3121 atcatggcgc agttttttca gggcatgaat gaaggattgc aaggcagcgc caaatgcacg
     3181 accaaaaaat tttccgatgc cgtacaaaaa gctacaaaat ccgcatggag cgcaatttcc
     3241 aatcccgttg aaggcaccat actctccgtc atacgcgact ggggaaaatc cgtagaaaaa
     3301 aatgccgaaa aaacggatga tttttccgaa ctattccgtg aaagtcttgc cgatgccaaa
     3361 cagtcgctcg ccaatacgcc caaacaaatg gccgctttag cgaaagccgg cgtcgtggat
     3421 gcgggcgcac aaggatttgt gaccatgctg gaaggcgtgc tggatttcat agaaaacgga
     3481 cgcatttccg acatcaagcg tcaaggccta tccgaaaccg aagacgaagc gatggaagca
     3541 cacattgatg agcacgcctt tgaagaaatc acgtatcagt tttgtaccga atgtttgctt
     3601 gtcggcgaaa acatggatcg cgaaaaaatc cgcgaaagtc tcaacaaatt cggcgactcc
     3661 ctgatcgtgg ccggctcaac caaaaaagtc aaaatccatt tgcactccaa tacaccggaa
     3721 gacgtatttg aagtattagc cgatcatgga cacgtgattt ccaccaaaat agacgacatg
     3781 aaacagcagt ttctcaatgc gcactccgag aatgtccaag agacggcaat tgccattgat
     3841 tcgtgctgcg atcttccgct cgaatatatc cgccgaaatc atatccatgt cattccactc
     3901 aacgtgcatt ttggcgaaca gatttatata gatcgcctta cgttgacacc gaagaaattt
     3961 tatgaaaaat tgacgtcttc acccgatcat cccaaatctt cgcaaccgac gccggctaaa
     4021 tttaaagaat catacgaacg actcttacaa atttacaaaa acgttgtttc cattcacctt
     4081 tcctccaaac tcagcggcac ctaccaaggc gccctcagta tggcacgtca ggtagcccaa
     4141 gataaaatat tttgttttga ttcgatgagt gcatccgtcg gcatcggcgt catggtgcga
     4201 gaaatcgagc cgatgattcg cgaaaacgct ccggccgaaa aaatcatgca acatctggag
     4261 tactgccgca ataacatgaa agtatttatc agcgtgcaga ccatggacta tctcatcaaa
     4321 ggcggccgcg taagcaaagc acgcggagcc atcgccaaat tattaaacat tcgcccggtg
     4381 ctaactgtac aagatggcgc cgtagtaccc atcgccaaag ctttcggcca ctccaatgct
     4441 ctgcgtaaag tgatggacct tgtcaaagca accaccaaaa acagaaaaaa gccgcgtttt
     4501 ggtatcgctc acgctaactc gatcggtatt gccgattggt atgcaacgca attaagccgg
     4561 tattatgaaa ttgaccgcga tgatatcctg atcaccgatg tatctaccgt catcgcaaca
     4621 cacgccggcc cgggtgcagc agctattgcg gtattaagcg atccgtagat accgtgacga
     4681 gccgcgtcaa tataacgatg cctcgaggtc cttacgaaaa gtggatcgaa gtaatttgca
     4741 tcagtgtcgt cgttgcctcc tttgttttgg tcatggggca tattcaagaa ttacccgaaa
     4801 gcatcgccat tcattttgat tggtcgggct atcctgacaa ttggggttcc aaacatacat
     4861 tatggctttt gcctgcgtta tcggctcttg taatatacat gcctttgaca gtattttctt
     4921 ttttgcaaca aggccatacg aaacccgacg acatttcctc gacgttaatt caccgcaact
     4981 ttaaaattgc acgcataagt ttggcgctga taaaacttaa tgtgctcttt cttatgtggt
     5041 ggttaacgta tatcgtgatt caatcggctt tatcgggtcg catagatcat aatccggtgg
     5101 gcgtgttatc catttttctt ttgattttga cgctgatcat tggtatttcc atctatcttg
     5161 taaaagccaa gcggagtaag ccaagtgcat aattggatta tcatattttt gttactgcat
     5221 gttatcccgg tgtcgagcca aaccatgagc gatccggggc taaaaaagta tatcggctta
     5281 tgggagcgcg aacaaaccga ttcgcttaaa acactcattg cggaaatgga aaaaaaattt
     5341 cccaaagcac ccgagacggt ttttctcaaa aacacatatg aaaccgatgg cgctatcgcg
     5401 tacgcggttt atctccgttt tgttcgcgac tatccgaaaa atattttcgc accggaagcg
     5461 tatcatcgta tgatccaata cgacgccgcg gccggccgtt atgcgttggc gcaggataat
     5521 ttgattaaat tgcgaacgga ttatcccggt tcatcatgca ttaagtcggc ggaaacactt
     5581 tttccggcga ccaaacccga cgaagcaaca caagccatta tgacagcccc gagcgataca
     5641 gcgcgtccta cggaaataaa tgccgggaaa aaatttcgat tgcaggtagg cgctttttcc
     5701 gatcaaaaaa acgcccagga acttaaacaa aaactgacag caaaaggata cgctccgatt
     5761 gaattttcgg agaaaacggt taacgggaaa actttgattt tggtatgggt cggcgcgtac
     5821 actacgcacg atgacgctaa aaaagccggt gatacgatca aaagtaaaga acaactcaat
     5881 tacagcatcg ttgaaaaatg aaactatccg cgcattcatt gcgctcgatt ttcctaacga
     5941 agccttttcc tttttttcag agcaagagag gcgtttaaag cccttattta acgacgcacg
     6001 catcgcttgg gtaaacccga ccaatattca tatcacgtta gctttcctcg gcgagataaa
     6061 tcgtgatgct atacccaaaa tcacacggtg tttatcggat ctggatgaag ccggcccctt
     6121 ttatttagcc gccacacacc ttggtttttt ttcgacgaaa ggaaatccca aagttttatg
     6181 gattggtttt aaaaaaacat cgtctattga aagtttgcaa aacaaaattt gcattcggct
     6241 caatgccctt ggtttgacaa cggaagaaaa aaaaattatt ccccacgcca ctttggctag
     6301 attgcgtgat tggccttccg cccgccttgc acagcaaaat gtcatccatc aatttatcgg
     6361 tgaaacagag tgggttatgc cgacgttgac tctttatgaa agcgtattac agcgtcaggg
     6421 agcgcaatac aaacccttat tccaaataaa attcacatga aaatcgcttc gatacaacaa
     6481 aagaaggtca aacgtaatca atcggcaacg gtttgtgttt tgacagaaga aggtgattca
     6541 ttcgaattac tctacgaaac cttggtgaaa tttggaatat atgacggctc cgatcttgat
     6601 gaaaaaaaat ggaaccgcat tcagctttgg gatagcacac ggcgcgccac gaccgacgca
     6661 ctgcgttggc tcagtcttcg tccgagaagc cgcgaagaaa tttcaaatcg tttgcgccgc
     6721 gaaggatata atgacgaaac cataacgtcc gtactggaaa aactgagtga agagaaatac
     6781 atagatgatt tggattttgc aatgcgattt gcgcagtccc gcctacgcaa aaaagccatg
     6841 ggtgaacgcg ccttagcata cgaactgaat aaaaaaggaa tcgctccgga cttgattcgg
     6901 caggtaattc aaaaatttgt acctgaaaac gaccttccgt cggttttacg gttagcggaa
     6961 aaaaagtatg cgacgattcg agaaagcgat caacgtaaaa aacagattaa actttcagca
     7021 tttttacaac aacgcggctt tgatacagat acgattcgta aggttgtaga gacgattgca
     7081 tcacatgaaa aaataatatc ccgcgatcaa aaccctctaa tcccgtcaaa ctttaaaaaa
     7141 cattaaactg ttctattttc tattga
//
LOCUS       DAOQNQ010000170         7073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_4056_length_7073_cov_5.762610, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000170 DAOQNQ010000000
VERSION     DAOQNQ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4056_length_7073_cov_5.762610"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            400..2439
                     /locus_tag="PK754_10550"
     CDS             400..2439
                     /locus_tag="PK754_10550"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HNB09988.1"
                     /translation="MLRSCFFIAIVCTVMYLGCAPQNVETLPDPLVHNRDSSVTPGQD
                     FFLHANGGWFKRNPIPESESSNGLWLTIGEETRAAVREICEKSAQETKAPKGSNKQKI
                     GDFYASGMDSAASEKAGISPLKSELDRIDAMKSISELAAVAARLHQINGDPIFSFNIY
                     KDDKKPTEYAVALNQGGLGLPDRDYYFKNDERTTGIRAAYQKHLFRNFEIVGESPDQA
                     RAFADHIFALEKNLAKASRTLEGLRDPYANYNKMSVAELSKLTPSFDWNVFFQNTGLK
                     NVDSVIVGQPEFFKEVENTVKNTSLDAWKAYLRWSVIGTYAAYLNQALVDENFNFYSK
                     TLYGVKQMKPRWKRVVERTNRALGEVIGQVYVEEYLPKGTKEKLLEIGNNIRDVCRER
                     IEKVSWMSDATRERALLKLQTMAMKVGHPDQWRDYSRLSVERDTYVANVMRANVYEFD
                     FMIGKYGKPVDRTEWNITPQTYNAYYDPTNNEIVVPAANIIVPGFGKTMPDDAILYGI
                     IGGSTFGHEIIHGFDDQGSQYDETGKLNNWWTKEDRARFEAKTKMLVTQFNNYVVLDS
                     VHLRGEATLGENIADLGGVELGYDAFKRSAQYKNNQKVAGLTPDERYFLAYAYAWMIQ
                     MRPEMLASRIMSDVHAPAQHRVIGPLSNNVKFYEVFGIKPGDAMYRPENERVSIW"
     gene            2888..5548
                     /locus_tag="PK754_10555"
     CDS             2888..5548
                     /locus_tag="PK754_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNB09989.1"
                     /translation="MKLSDPFKILTPNERWAPTQSQMDAFQNAYEKLLPPLVYKIRLA
                     VARWRDEGYQGASNTSKSLLNFWFNQEHLIGQSKFSFFFAQREAIESIIYLYEIARAK
                     DKYELMRFDSSQRVSTGMFDENWTRYVIKMATGAGKTKVMGMTLVWSYFHKLYEADST
                     LSKDFLVIAPNIIVLNRLRKDFDGLKMFFDEPFFPDNGYDDKDWKNDFQLTLHIQDDL
                     KPITESGNIFLTNIHRVFFNEAPEPNFETTFLGVKPKPDADTSKGLDLGKVLRSDKIK
                     GLVVLNDEAHHIHDNSLAWFKSIEDISNKLKLKNGNGISLQADYSATPKHNNGAIFVQ
                     TICDYPLVEAIKQKVVKSPVLPDEASRQKIQEKDSSDFVERYRDYMHLGYLEWEQQYE
                     ELKSQKTPILFIMTMNTKEADQAAAFLEASYPKMKNSVLTIHTNNSGEIKETATSKKD
                     KEELEALRKAADEIDKDHSPYKAVVSVLMLREGWDVKNVTTIVGLRPFGADSKILPEQ
                     TIGRGLRKMFSLDMPEKLVVVGTPAFLEFVESLKTEGVEFQYSPMGKGSKGKSPVIVE
                     VDKENPNKDLDVLDIPIPQMSPRIYREFKNLELIDVSKFKNEKVIQKKFNSDELKEIV
                     FHDIDGDFSHKTIYKETTPDYRNVISFFTSTILKDSRLVSGFNILYPKVESFIKYQLF
                     TKEVELSNPQTMRNLSEIQPKEILKTTFKKAIDDLTITDKGTAEVKNFISLKLTKPKV
                     AENQPFLVPKKSVFNKIIGENPFELEFSAFCENRFNDVVSFAKNTMGEGGINFKIEYQ
                     ATDGNIREYFPDFFVKIDNRTFFVVETKGRQDLDDILKIKRLASWCHDINNAQKEYTY
                     TPVYIKQEKWDDIKDNLKSFQDLKTIFEVK"
     gene            5545..7005
                     /locus_tag="PK754_10560"
     CDS             5545..7005
                     /locus_tag="PK754_10560"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB09990.1"
                     /translation="MKPWVTKALHYLEQSLGEIPQELNELDWKEDLSPNNEKLSRHLS
                     AFANHPGGGFLVFGIDNISAKPVGLTRESVSIILDRLSNLCRNGVDPVVVIDHIVHPF
                     RDVQLLIIHIKESAVKPVHIANKTMEESYIRTGSSTRKASRQEIGGLMLNSKTPVFEE
                     LHASKLKNAVEIMTALDFAAIYKLLKKPVPTNLAEIIRWLQDEKMIVQIEDDGFYITN
                     FGALSAAQNLNDFDNLSRKSIRLIKYEGNNKTVQSKEFPGSKGYAIGFEGLIQFIKGI
                     LPGSEIIKNALRAETSIFPEIALRELIANALIHQDFSIRGSGPMVEIYDDRVEITNPG
                     KLLPNKNIDRLIRTTPESRNEILAAAFRRYNICEERGSGFEKAVTAIELFGLPPLRFE
                     ETDGSFKVVMYAPKPFADMSDKERIEACYQHCIIQYYGQGGMSNSSLRKRFGMHDKQA
                     SQVSRLIKLAIETGRIKYKDPNNQSTKLNEYIPYWA"
BASE COUNT         2232 a         1475 c         1475 g         1891 t
ORIGIN      
        1 gacttgttct ccttgttttg tttttatgtg ttattagtaa attctttttc gtttcatttt
       61 ttgtatcatt tccatcatcg taacatgatc aatttatagt cagctgacgg cccttttctg
      121 tcaatttttg acaatattta ccccatccct gcattttttg attttgccgt ttatcaccgc
      181 acgtttagca ttttggggta ctaaatattc acaagccgct ccggttaatc ctctatgctc
      241 tgagccgata catcctgtaa cctttactcc ggctttgcgt atcttttttc gatttttatg
      301 atagaccgct aatcctcgta atacggattg atttattact aaaacctgtt aatttttatg
      361 cgttgtaagc atactgaata cagaaaggca ccctttacca tgttacgttc atgttttttt
      421 attgcgatag tctgcacggt tatgtatctg ggttgtgcac cccaaaacgt cgaaacactc
      481 cccgatccgc tggttcataa ccgcgacagt tcggtcaccc ccggccaaga ttttttcctg
      541 catgccaatg gcggctggtt caaacgcaac cctattcctg agtcggaatc ctccaacgga
      601 ttgtggctga cgatcggcga agagacacgc gccgccgtac gggagatctg cgaaaaatcc
      661 gcacaagaaa ccaaagcgcc caaaggcagc aataaacaga aaatcggtga tttttacgcc
      721 agtggtatgg atagcgccgc atcggaaaaa gccggtatct cgcccttaaa atcggaactg
      781 gatcgcattg acgccatgaa gtccatcagc gaattagccg ccgtagccgc acgcctgcat
      841 cagatcaacg gcgatccgat tttttcattt aatatctaca aagacgataa aaaaccgacc
      901 gaatacgccg tcgcgctcaa tcaaggcgga ctggggttgc cggatcgtga ctattatttc
      961 aaaaacgacg aacgcaccac cggcattcgt gcagcgtatc aaaaacattt gtttcgcaat
     1021 tttgagatcg tcggagaatc gcccgaccaa gcgcgcgctt tcgccgatca tattttcgct
     1081 ttggaaaaaa acttagccaa agccagccgc acgctcgaag gcctgcgcga tccgtatgcc
     1141 aactataaca aaatgagtgt cgccgaactt tccaaactca ccccgtcgtt tgactggaat
     1201 gtctttttcc aaaacaccgg cctgaaaaac gtagattccg tgatcgtagg acaaccggaa
     1261 ttttttaaag aagtggaaaa caccgtcaaa aatacttctc tggatgcatg gaaagcctat
     1321 ctgcggtgga gtgtgatcgg cacgtacgcc gcttacctca atcaagcgct ggtggacgaa
     1381 aattttaatt tttattccaa gaccctgtac ggcgtcaaac aaatgaaacc gcgctggaaa
     1441 cgcgtcgtcg aacgcaccaa tcgcgctttg ggcgaagtca tcggccaggt ctatgtagag
     1501 gaatatctgc ccaagggtac caaagaaaaa ttactcgaaa tcggaaacaa tatccgcgac
     1561 gtatgccgcg aacgtatcga aaaagtaagc tggatgagcg acgcgacacg tgagcgcgct
     1621 ttgctcaaac tgcaaactat ggcgatgaaa gtcggacacc ccgaccaatg gcgcgactac
     1681 agccgtctgt ccgtcgaacg ggacacctat gttgccaatg tgatgcgcgc caatgtgtat
     1741 gaatttgatt tcatgatcgg caaatacggt aaacccgtag atcgcacgga gtggaatatc
     1801 accccgcaaa cgtataatgc gtattacgat ccgaccaata acgaaatcgt ggtacccgcc
     1861 gccaatatca tcgtcccggg ttttggtaaa acgatgcccg acgatgcgat cttatacggt
     1921 attatcggcg gcagcacgtt tggtcatgag atcatacacg gcttcgatga tcagggcagc
     1981 caatacgacg aaacgggcaa actgaataac tggtggacca aagaagaccg cgcccgtttt
     2041 gaagccaaaa ccaaaatgct ggttacgcag tttaataact acgtggtact cgacagcgtg
     2101 cacctgcgcg gcgaagccac gctgggcgaa aacatcgccg atctcggcgg cgtggaactg
     2161 ggctacgatg ctttcaaacg ctccgcccaa tacaaaaaca atcaaaaagt agccggtctg
     2221 acgcccgacg aaaggtattt cctcgcgtac gcgtacgcat ggatgatcca gatgcgcccg
     2281 gaaatgctcg ccagccgcat catgtccgac gtacacgcac cggcacaaca ccgcgtcatc
     2341 ggcccccttt ccaataatgt caagttttac gaagtttttg gtatcaagcc cggcgacgcg
     2401 atgtacagac ccgaaaacga acgggtcagc atctggtaga tcagcggtgt tttaaatttt
     2461 tttatcaaat ggaaacgtgt ttatcgggtt ggctcaccga tgaaagagag tccatccgtc
     2521 ttgagcaaag tcgaagacgg atggcactaa accgggccat ggctcccccc ccccacgcac
     2581 catgacgtat gggagttttc ccgataagca caaataaaaa taaatatagc caatataaaa
     2641 aattggctat atattttttt ttacagggtc agtggagcgt ggcacgggca gataatcgct
     2701 gtacgtcagg gtcgatatgt agccagtcat aagtcacagt atgaggaaac atgcgttttt
     2761 ttactttatt tttttctctc aatcgaaagc ccgacacaca agccaacgtt tagcaaaacc
     2821 aaaagagcca ctttttagcc aacgcgccga cataaatatt aactttgaaa caaaagaaaa
     2881 taggtaaatg aagttatcag acccgtttaa aatattgaca ccgaatgaaa ggtgggcgcc
     2941 tacacaaagc caaatggacg cttttcaaaa tgcctatgaa aagttgttgc cacctttggt
     3001 ttataaaatc agacttgcgg ttgccaggtg gagagacgaa ggctatcaag gggcttcgaa
     3061 tacttcaaaa tcattattaa acttttggtt taatcaggaa catcttatcg ggcagtcaaa
     3121 atttagtttt ttctttgcac aacgagaagc cattgaatca attatttact tgtatgaaat
     3181 agccagggca aaagacaaat acgagttaat gcgatttgac tcttcccaac gtgtcagcac
     3241 tggtatgttt gatgaaaact ggacacgtta tgtaattaaa atggctacgg gagcaggtaa
     3301 aacaaaagta atgggtatga ctttggtttg gtcttacttt cataaacttt acgaagcgga
     3361 cagcacacta tcaaaagatt ttctagttat tgcacccaac atcatcgtat taaaccgctt
     3421 acgtaaagac tttgacggac ttaaaatgtt ttttgatgag ccgtttttcc ctgacaatgg
     3481 ctacgacgat aaagactgga aaaacgattt tcaattaaca ctgcacattc aagacgattt
     3541 aaaaccaata acggaatctg gcaatatttt ccttaccaac attcatcgtg ttttctttaa
     3601 cgaagcacct gaaccaaatt ttgaaacgac ttttttaggc gttaaaccaa aacccgatgc
     3661 agacacttca aaagggttag atcttggtaa agtattgcga agcgacaaga ttaaaggctt
     3721 ggttgttttg aatgacgaag cccatcatat acacgataac tcattggcat ggttcaaaag
     3781 cattgaagac atcagtaaca aattgaaatt gaaaaacggt aatggaataa gcctgcaggc
     3841 agattattct gctactccta agcataacaa tggtgctatt tttgttcaaa ccatttgcga
     3901 ttatcctttg gttgaagcta tcaagcaaaa agttgtaaag tcgcctgtat tgcctgacga
     3961 agcatcaaga caaaaaattc aggaaaaaga ttcttccgac tttgtagaac gctaccgaga
     4021 ttatatgcac ttaggttatt tagagtggga acagcaatat gaagaactaa aaagccaaaa
     4081 aactccgata cttttcatta tgacaatgaa caccaaagaa gcagaccaag cggctgcctt
     4141 tttggaggcg agttatccta aaatgaaaaa ttcagttttg acaattcaca ccaacaattc
     4201 gggcgaaata aaagaaactg ccacaagcaa aaaagataaa gaagaattag aagcattacg
     4261 aaaagcagca gacgaaatag acaaagacca ttcgccttac aaagcagttg tctcagtttt
     4321 aatgcttcgc gaaggttggg atgttaaaaa tgttaccacc attgttgggc ttcgcccatt
     4381 tggtgcggat tctaaaatcc ttcccgaaca aactattgga agaggtttgc gaaaaatgtt
     4441 ttcgttggat atgcccgaaa aattagttgt agttggaaca ccagcgtttt tagagtttgt
     4501 cgagagcctg aaaacagaag gtgtagaatt ccaatacagc ccaatgggca aaggctctaa
     4561 aggcaaatcg cctgtaattg tggaagtcga taaagaaaat ccaaacaaag atttagatgt
     4621 tttggatatt cctatcccgc aaatgagtcc aagaatttac agagagttta aaaaccttga
     4681 attaattgat gttagcaaat ttaaaaacga aaaagtcatt cagaagaaat ttaacagcga
     4741 tgaattaaag gaaattgtat tccatgatat agatggtgat ttttcacaca aaaccattta
     4801 taaggaaaca acccctgatt atagaaatgt gattagtttt tttacttcca caattttaaa
     4861 ggacagtcgc ttggtaagcg gttttaatat tttatatccc aaagtcgaaa gctttataaa
     4921 ataccagctc tttacaaaag aagtggaatt aagtaatccg caaacgatga ggaacctttc
     4981 agaaatacaa ccaaaggaaa ttttgaaaac aacgttcaaa aaagccattg acgatttgac
     5041 gataactgat aaaggaacag cggaggttaa aaactttatt tcacttaaac taacaaaacc
     5101 gaaggttgca gaaaaccaac cattccttgt accaaagaaa tcggtcttta ataagattat
     5161 tggagaaaac ccttttgaat tggaattttc tgctttttgc gaaaacaggt ttaatgatgt
     5221 tgtttcattt gctaaaaata cgatgggtga aggcggtatc aatttcaaaa ttgagtatca
     5281 agcaactgat ggaaatattc gcgagtattt tcctgacttt ttcgtgaaga ttgataaccg
     5341 tacttttttt gttgttgaaa caaaaggacg ccaggatttg gatgatattt taaaaatcaa
     5401 gcgattggct tcatggtgtc atgatattaa taacgctcaa aaagagtata cctacacccc
     5461 tgtttatata aagcaggaaa agtgggatga tattaaggac aatcttaaat catttcagga
     5521 tttaaaaacc attttcgagg ttaaatgaaa ccctgggtta ctaaagcgtt acattattta
     5581 gagcagagtc tgggcgaaat tcctcaggaa ctaaacgagc ttgactggaa agaagattta
     5641 tcacctaaca atgaaaaact ttccagacat ttgagtgcgt ttgccaatca ccccggcggt
     5701 ggattccttg tatttggcat agataatatt tctgcgaaac ctgttggcct aacaagagaa
     5761 tccgtaagca ttattcttga tagactttcc aatctttgcc gaaatggtgt tgatccggtc
     5821 gttgtgatcg atcatatcgt ccatccgttt cgtgatgtac aacttcttat tattcacata
     5881 aaagaaagtg ccgtcaagcc agtccacatc gcaaataaaa cgatggaaga aagctatatc
     5941 agaaccggtt catcaacccg aaaagcatcc agacaggaaa tcggtggttt gatgttaaac
     6001 agcaaaacac ccgtttttga ggaactccat gcttcaaaac ttaaaaatgc tgtcgaaatt
     6061 atgacggctt tagattttgc agccatttat aaactactga aaaagcccgt tccaacaaac
     6121 ctcgcagaaa ttatccgttg gcttcaggat gaaaaaatga ttgttcaaat tgaagatgac
     6181 ggattttata tcaccaattt tggtgcgctt tcagccgctc aaaacttaaa cgatttcgat
     6241 aacctgtccc gaaaatcgat ccgccttatc aaatatgagg gaaataataa aacggttcaa
     6301 agcaaagaat ttcccggttc gaagggttat gcgatcggtt ttgaagggct gattcaattt
     6361 attaaaggta ttctgcccgg tagtgaaatc atcaaaaatg cactgagagc tgaaacctct
     6421 atttttcctg aaatcgcact tagagagctc atagccaatg cactcattca tcaggatttt
     6481 tctatcagag gttccgggcc tatggttgaa atttatgacg atagagtcga aatcaccaac
     6541 ccgggtaaat tgctgcctaa caaaaatatt gaccggctga ttcggacaac acctgaatca
     6601 cgaaatgaaa ttttggctgc cgctttcaga cgatataata tctgtgaaga acggggttca
     6661 ggttttgaaa aagcagtcac cgccatcgaa ctttttggtt taccgccttt aaggtttgaa
     6721 gaaactgacg ggtcatttaa agtcgtcatg tatgctccga aacctttcgc tgacatgtcg
     6781 gacaaggagc gaattgaggc gtgttaccag cattgtatta ttcaatacta cggacaaggc
     6841 ggcatgagta attcatcgtt acgaaaacgg tttggaatgc atgacaaaca ggcatcacaa
     6901 gtttcacggt tgattaaact ggcgatagaa acaggcagaa ttaaatataa agatccgaat
     6961 aaccagtcga caaaattaaa tgagtatatt ccgtattggg cttaacttgc ttgattgggt
     7021 tagggagtta atttgtaagt tattgttaat caataatatg ctgtttgact gag
//
LOCUS       DAOQNQ010000171         7012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_4102_length_7012_cov_6.809976, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000171 DAOQNQ010000000
VERSION     DAOQNQ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7012
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4102_length_7012_cov_6.809976"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PK754_10565"
     CDS             complement(<1..288)
                     /locus_tag="PK754_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09991.1"
                     /translation="MTTPRLFAIMGIFFCTMIAWFILAGSISFRTDEKRLSFGPRMAS
                     HWGGVHKQETPQLFYHVKEWAVTKETSTNAAGASVTVEKSSEVDVKKNIECG"
     gene            complement(285..710)
                     /locus_tag="PK754_10570"
     CDS             complement(285..710)
                     /locus_tag="PK754_10570"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNB09992.1"
                     /translation="MNHRISRISIFHKVPSRLQWLIAYIGFLSLVSISAYSYGLPRWL
                     DIIPFYDTIGHFVLMGILGILTLLVLRRTIRIKLDISVALAPIVISLISIADEYLQKL
                     SPHRTFSYLDMAANLIGIWTFYFIYRFYAKSKRVKEESL"
     gene            complement(707..1000)
                     /locus_tag="PK754_10575"
     CDS             complement(707..1000)
                     /locus_tag="PK754_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNB09993.1"
                     /translation="MKTEIHHIDEVIHQKVRLGIMTILAARRDADFTELKQELTLSDG
                     NLSTHLSLLEKHHYITIQKQFSGRKPQTTIAITEIGKTALKNYLTILRKIIES"
     gene            complement(1015..1623)
                     /locus_tag="PK754_10580"
     CDS             complement(1015..1623)
                     /locus_tag="PK754_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB09994.1"
                     /translation="MNKSNAKEQLDYIQTVLDRATVYTNISGWSAISVGLLGTIAGFY
                     SEWALKGSLDYQTYIASLVGCWSFCFIASIALAVFFSARRAKRMNEKIWGEPIKQAVR
                     HFSVFVFIGGVQTAACTFYHAYYLIPSVWMLCYGGGIIVGGLYSVKEIRILGLFFLAA
                     GSIALFSPVWNQIALMSTFGFGHIILGLIILKKYNQPLSHKS"
     gene            complement(1845..2792)
                     /locus_tag="PK754_10585"
     CDS             complement(1845..2792)
                     /locus_tag="PK754_10585"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB09995.1"
                     /translation="MIAVHLALFFVSLVFGVNAVTSKYAMREIHPMALVFIRIVSAAA
                     ILYIIQRLWIKEKIQTRRDYFLLAGYSIVGIAINQVLFLKGLALTTAINATVLITTMP
                     AATIGIAILLRKEPPSFRKIIGSMISFSGVILLLGSERIDFSNDYFVGNLLVFLNGIS
                     YSFYLVISRDLLKRYHPSTVTTWIFVFGAVGVFPFGIGEISRLDWASVSLTAWACVAF
                     LIIFSSVIVYFINNWALRKTSSSVVAVYIYVQPIVAALISTQMMGETLRMETVIAALL
                     IFGGVFIVTLKMREEMKKEKSNANKEIDLVKESMIMKED"
     gene            complement(2799..3134)
                     /locus_tag="PK754_10590"
     CDS             complement(2799..3134)
                     /locus_tag="PK754_10590"
                     /inference="COORDINATES: protein motif:HMM:NF025852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNB09996.1"
                     /translation="MSSDRVIEINTDSVAWESSDSKGVFYKSLRYDKETKAGAVLIRM
                     EPGSDYPEHEHTRGEDFIVLEGELIIGNKTFPAQSYVYSPPQSVHHPRTEKGCVLFAV
                     FHGKIINLK"
     gene            complement(3140..5407)
                     /locus_tag="PK754_10595"
     CDS             complement(3140..5407)
                     /locus_tag="PK754_10595"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB09997.1"
                     /translation="MNTQSLEFVQKTKQELNCKIIRTMLNYALEKAGPDAERKLIERM
                     GLTREYLQNENNWVSFDVYTHACETMKTIFNDDRIMYRVGIYSFSNLENFGAIAKMAV
                     YLLKPESIYRRTAEVANQAVKFGTYRVVEIESNRLLLEFKNREGYKFHQHNCDYRLGV
                     FAGIPQLFGLPIARHRSIQCVSDGADRCLYEFRWLSRTWEIFGRHARIGLVMGILFAL
                     VLTVLNRFTFSHFSQPALLISGAAVLYLIGIILDMKHTAQYSAFINSGKSDEIEEAMA
                     FANQKYQEAADNITKLQAIIEGNAVLQSKLIKSELVEIVLEIIKHNLGYDRAMILFKN
                     NTQPLLDSPKLIGNLSPEMEQALLNYKINLTDDTAINTRVFYSGEPALITDMSEVKNS
                     TEMKAVQLSGTKSFVILPFKIGSRVVGTLTVDKISSPKALSREDVRILNTLLNMLSVA
                     LENAELYQDLEARVRERTAQLNETNKKLESAYDELKQAQAQLLHNEKMASLGKLVAGI
                     AHEMNNPAGILSGYIELMELHANRLRKDLEILDQFSAAHEDIAGTIQHIEKNILEITD
                     SIEPCKRSVWRIKSIIQDLRNFSRLDENELMPANINEGLENTLKFFKNQAEGRIRIIT
                     HFNPIPQVTCFPGHLNQVFSNVISNAIEAIDGTGTIEIHTSKSEPTSDVPTDHVVVKI
                     KDSGHGIPAEIQGKIFDPFFTTKDIGAGKGLGLSIAYGIIKQHSGNISAESEQDQGAC
                     ITIRIPIEYGYERKG"
     gene            complement(5404..>7012)
                     /locus_tag="PK754_10600"
     CDS             complement(5404..>7012)
                     /locus_tag="PK754_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNB09998.1"
                     /translation="VPAAVAIGTNVFDQFLEHNNLRELALNSTDDREIADSFHSASYF
                     PPEVITQLREFLEMVCVPLAVRSSSLLEDSQYHPFAGVYETYMIPNNHADTETRLQEL
                     LTTIKKVYASTFYKATKDYIKQTAYRLEEEKMAVIIMRTVGAAHDNRFYPDFSGVAKS
                     YNFYPMPPQTAKDGIASIALGLGKTIVDGGNTVRFCPKYPKHLIQQFSTKEALKTSQT
                     EFYAMKLGATEVDLYRDTKDRLIGKFTLDTAEKDGSLRFSGSTYSIENDAVYDGISRA
                     GMRIVTFAPILKNKMFPLPEILDLLLELGKWGMGSEIEMEFAVNMSTPPGKPREFGVL
                     QMRPLVLRLENDALDVTPYRREEMICESTQVLGNGVIDSIRDILVVDYDRFDRARSKE
                     VALEIAQLNEKLVRDGKPYALIGVGRWGSLDHWLGIPVRWDQINGARAIIESSFRDFD
                     VDPSQGSHFFQNITSFQVGYFTVPHHAASSFVDWAWLAAQKAEEELNFTRHLSFENPI
                     VAVINGHDGKGVLLKPKPQPKESAVSL"
BASE COUNT         1963 a         1582 c         1559 g         1908 t
ORIGIN      
        1 accgcattcg atattttttt tgacatccac ctccgaactc ttttctaccg tcacgctggc
       61 accggcggca ttggtactgg tctctttggt gaccgcccat tctttgacat gataaaacaa
      121 ttggggtgtt tcttgtttgt gtacgccgcc ccagtgcgag gccatacgcg gaccgaagct
      181 caatcttttt tcatcggtac gaaacgaaat actgcccgct aaaataaacc aggcgatcat
      241 cgtgcagaaa aaaataccca ttattgcaaa caaacgcggc gtagtcataa cgattcctcc
      301 tttacacgtt tacttttggc ataaaagcga tagatgaaat aaaaagtcca gataccgatc
      361 agattggccg ccatgtcaag gtaactaaac gtccgatgcg gggatagttt ttgtagatat
      421 tcgtctgcaa tagaaataag gctgatcact atcggagcga gagctacgct aatatctaat
      481 tttatgcgta tggtgcgtcg taaaaccagc aacgtgagaa ttcccaaaat gcccataagc
      541 acgaagtggc cgatggtatc ataaaaggga atgatatcaa gccaacgagg caaaccataa
      601 ctatacgccg aaatgctaac gagcgataga aatccaatat atgcgatcaa ccactgaagg
      661 cggcttggca ccttgtgaaa gatgctgatc cgggaaatgc gatgattcat gattcgatga
      721 tctttcgtaa aatggtaagg taatttttta aagccgtttt accgatttcc gttatagcaa
      781 tggttgtctg aggtttcctt ccgctaaact gtttctgaat ggtgatgtag tgatgttttt
      841 caagcaatga caggtgggta ctgagattgc cgtccgaaag cgttaattct tgtttgagct
      901 ccgtaaaatc cgcatcgcgc cgtgcagcca aaatggtcat aataccaagc cgcacttttt
      961 ggtgaatgac ttcgtcaata tgatgaattt cagttttcat aaagtgtttc gtcgctaaga
     1021 cttgtgcgat aacggttggt tatacttttt cagaatgatc agtcccagaa taatatgacc
     1081 gaaaccgaag gtgctcatca aagcgatttg attccaaacc ggtgaaaaaa gagcaatact
     1141 tccggcggct aggaaaaata atccgaggat tcgaatttct ttgacggaat acaatccgcc
     1201 gacaatgata ccgcctccgt agcagagcat ccaaaccgac gggatcaaat aatatgcatg
     1261 atagaatgta caagccgcag tttgcacgcc gccgataaaa acgaatactg aaaaatgccg
     1321 tacggcttgt ttgatcggtt cgccccagat tttttcattc atacgctttg cgcgtcgggc
     1381 tgaaaaaaat accgctaatg cgatcgaagc gataaaacaa aaactccaac acccaaccaa
     1441 cgatgcgata taagtttggt agtccagcga tcctttgagc gcccattctg agtaaaagcc
     1501 tgcgatcgtg cccaaaaggc ctaccgaaat ggctgaccaa cctgagatat tggtataaac
     1561 agtggcccga tccaatacgg tctggatgta atcaagttgt tcttttgcgt tggatttatt
     1621 cattgtaaaa ccttatttat taactttgca ttacaaagta ctttgtaaat aaagcaaaat
     1681 acaaatgaag agtcaagttg tttttatatc tcagtgaaag aggtttgatt ggttaagatt
     1741 gaggccttac cggatatcgg gttttcaaaa aaggaaaggg atgtgatgta tttgtaagca
     1801 acgatgagtt aggagcacaa taccgtgaac aagtctttaa acgatcaatc ttctttcatg
     1861 atcatggatt ctttgacgag atctatttct ttgttggcgt tacttttttc ttttttcatt
     1921 tcctctcgca ttttaagcgt gacgataaat acgccgccaa aaatcagcaa cgcagcgatt
     1981 actgtttcca tgcgcagggt ttcacccatc atttgtgttg aaatcaacgc cgcaacaatc
     2041 ggttggacat aaatataaac agcgacaacc gatgacgatg ttttgcgcag tgcccagtta
     2101 tttataaaat aaacgatcac agacgaaaaa ataatcaaaa aagccacgca tgcccatgcc
     2161 gtgagactga cggacgccca atccaggcgc gatatttcgc cgatgccaaa cgggaaaaca
     2221 ccgacagcgc caaaaacaaa tatccatgtt gttacggtcg aagggtgata acgcttcaaa
     2281 agatcccgtg agataaccaa ataaaaactg tacgaaatgc cgttaagaaa aacgagcaga
     2341 ttaccgacaa aataatcatt gctaaaatct atacgttcgc tgccgagtaa caggataacg
     2401 ccgctaaagg aaatcatcga accgataatt tttcgaaagc ttggcggttc tttgcgcaga
     2461 agtatagcga taccgatcgt tgcggcaggc atggtcgtga tcagtacggt cgcattgatg
     2521 gctgttgtca aggcaaggcc tttaagaaaa agtacctgat tgatcgcgat accgacgatg
     2581 ctgtaccccg ccagtaaaaa ataatccctg cgcgtttgaa ttttctcttt gatccaaagc
     2641 cgttgtatga tgtagagaat cgcggcggcg cttacgatgc gaataaacac cagagccata
     2701 gggtgaattt cacgcatggc gtatttggaa gtcaccgcat tgacgccgaa aacaagcgac
     2761 acaaaaaaca aagccagatg tacagcgatc atgggaggtt atttaaggtt gatgattttc
     2821 ccgtgaaata cggcaaaaag tacacatcct ttttccgtac gcgggtggtg tacggattgt
     2881 ggcggactgt atacgtaact ctgtgcaggg aatgttttat tcccgatgat caattcacct
     2941 tctaatacga taaagtcttc accgcgtgta tgctcatgtt caggataatc ggatcccggt
     3001 tccatacgaa tcaaaacggc gcccgctttg gtttccttat catagcgaag agatttataa
     3061 aaaacgcctt tactgtcgga tgattcccat gccacgctat ccgtatttat ttcgattacg
     3121 cgatcactgc tcatacggtt tatccttttc tttcatagcc atattctatc ggaatgcgaa
     3181 tcgtaataca cgcgccttga tcttgttcac tttcggctga aatatttccg ctgtgttgtt
     3241 tgatgatacc ataagcaatg gataaaccta atcctttgcc tgcgccgata tctttggtgg
     3301 taaaaaatgg atcgaagatt ttaccctgaa tttcagccgg aatgccatga cctgaatctt
     3361 tgattttgac gacgacatga tcagtcggca catccgatgt aggttcgctt ttagaagtgt
     3421 gtatttcgat cgtgccggtt ccatcaatcg cttcgatagc gtttgaaatg acattgctga
     3481 acacttgatt gagatgaccg gggaaacaag tgacctgtgg gatcggattg aaatgcgtta
     3541 tgatgcgaat gcgcccttcg gcctggtttt taaaaaactt aagcgtattc tcaaggcctt
     3601 cattgatgtt ggcgggcata agttcgtttt catccaaacg ggaaaaattg cgcaaatcct
     3661 gaatgatact cttgatacgc cagaccgatc gtttgcacgg ctcgatcgaa tcggtaattt
     3721 caagtatgtt cttctcgatg tgttgtatgg taccggcaat atcttcgtgc gccgcgctaa
     3781 attgatccaa aatttccaaa tctttgcgaa gccgattggc atgaagctcc ataagttcga
     3841 tatatccgga aagtatgccg gcagggttat tcatttcgtg cgcgataccg gctaccaatt
     3901 taccaaggct cgccattttt tcattgtgta ataattgtgc ctgtgcttgt ttgagctcat
     3961 cgtaagcgga ttccaatttt ttgttggttt cgttgagttg ggctgtacgt tcgcgaacgc
     4021 gggcttccaa atcctgatac aattcggcgt tttccaaagc gacggataac atattgagga
     4081 gtgtattgag aatacgcaca tcttcgcgtg acagggcttt cggagaactg attttatcaa
     4141 ccgtcaaagt tccgaccaca cgcgaaccga ttttaaatgg taaaatgaca aatgattttg
     4201 taccggacaa ttgcacagcc ttcatttcgg tactgttttt cacttcagac atatccgtga
     4261 tcaatgcggg ctcgccgcta taaaaaaccc gtgtattgat ggccgtatca tccgtaaggt
     4321 tgattttata attaagcagc gcctgctcca tctccggact gaggttgccg atgagtttag
     4381 gcgaatccaa caaaggttgc gtattgtttt taaagagaat catggcgcga tcataaccga
     4441 gattatgttt tatgatctca agaacgattt cgaccaactc ggacttaata agtttcgatt
     4501 gaagtacggc attaccttcg atgatcgctt gaagcttggt tatattgtcg gccgcttcct
     4561 gatatttttg attagcaaac gccatcgctt cttcgatctc atcggatttg cccgaattaa
     4621 taaacgcact gtactgtgcc gtatgtttca tgtcgagtat gataccgatc agatacaaaa
     4681 cggccgcacc gctgatcaat aaagccggtt ggctaaaatg tgaaaaagtg aatcggttga
     4741 gaacggtcaa aacgagcgca aataaaatac ccataacaag accgattcgt gcatgccgtc
     4801 cgaatatttc ccatgtacgc gataaccaac gaaactcgta caaacaccga tccgcaccat
     4861 cggaaacaca ttgaatgctg cgatgacgtg cgataggcaa tccgaaaagt tgcggtatcc
     4921 ctgcaaaaac gcccagacga taatcgcaat tatgttgatg aaatttataa ccctcgcgat
     4981 ttttgaattc taacagaagg cgattacttt caatttcgac cactcggtat gtgccgaatt
     5041 tgacagcctg gttggcgact tcagccgttc gccggtaaat gctttccggt ttgagaagat
     5101 agacagccat tttggcgata gcgccgaagt tttcaagatt ggaaaatgaa taaatcccga
     5161 cgcggtacat gatgcggtca tcgttgaaaa tcgttttcat ggtctcgcag gcatgcgtat
     5221 aaacgtcaaa cgaaacccaa ttgttttcat tttgcaaata ttcgcgcgtc agccccatgc
     5281 gctcgatcag cttgcgttcg gcgtcaggcc ccgctttttc gagtgcataa ttcaacatcg
     5341 tgcgaatgat tttacagttc aattcctgtt ttgttttttg aacaaactca agcgattgcg
     5401 tgttcataaa gacaccgcac tttctttcgg ttgaggtttc ggtttcagta aaaccccttt
     5461 tccatcatgt ccgttgatca ccgctacgat aggattttca aacgacaaat gtcttgtgaa
     5521 attcagctct tcttcggctt tttgcgcggc tagccaagcc caatctacaa agctggaggc
     5581 cgcatgatgc gggacggtaa agtagccgac ttgaaaactg gtgatgtttt gaaaaaaatg
     5641 cgaaccttgc gacggatcca catcaaaatc acgaaaactg gattcgatga tcgcccgggc
     5701 gccattgatc tgatcccatc gtacgggtat gcccagccag tgatccaaac tgccccagcg
     5761 tcccacaccg atcaacgcat aaggcttgcc atcgcgaact aatttttcat tgagctgagc
     5821 gatttccaac gcgacctctt tgctgcgggc gcgatcaaaa cgatcataat ccacgaccaa
     5881 aatatcgcgg atcgaatcaa taacgccgtt acccagcact tgcgtacttt cacaaatcat
     5941 ttcttcccgc cggtacggtg tcacatccag ggcgtcattt tccaaccgca aaaccagagg
     6001 acgcatttgc agcacgccaa attcgcgcgg cttaccgggc ggagtggaca tattgacggc
     6061 aaactccatt tcgatttccg aacccatgcc ccactttcca agttccaaaa gcaagtccag
     6121 gatttcaggc agtggaaaca ttttattttt taaaatcggc gcgaacgtaa caatacgcat
     6181 accggcgcgg gaaatgccgt cgtacaccgc gtcattttca atagaatagg tcgaacctga
     6241 aaaacgtaat gatccgtctt tttcagccgt atccaacgta aacttcccaa tcaggcgatc
     6301 tttggtgtca cgatacaagt ccacttccgt agcgccgagc ttcatagcgt aaaattcggt
     6361 ctggctggtt ttgagcgctt ctttggtgga aaattgttgg atcaaatgtt ttggatattt
     6421 ggggcaaaaa cgaacggtgt tgcctccgtc cacgatagtt ttaccgagcc ccagcgctat
     6481 ggatgcgatt ccatctttag cggtctgtgg cggcatcggg taaaaattat aagacttcgc
     6541 cacgccggag aaatccgggt aaaaccgatt atcgtgtgcc gcgccgacgg ttcgcataat
     6601 aatcaccgcc attttttctt cttcaaggcg gtaggcagtt tgtttgatat aatctttggt
     6661 cgctttgtaa aaagtcgaag catacacttt tttgatcgtc gtgagcagct cctggagtcg
     6721 cgtctctgta tcggcgtgat tgttggggat catgtaggtt tcgtacacgc ccgcaaaagg
     6781 atgatattgc gaatcctcaa gcaaactgga cgagcgtacg gcgagtggaa cacagaccat
     6841 ttccaaaaat tcacgtaatt gcgttatcac ttcgggcgga aaataagaag ccgaatgaaa
     6901 ggagtcagcg atttcacgat cgtcagtcga atttaacgcc aactcacgca aattattatg
     6961 ctccaaaaac tgatcgaaga cattggtgcc gatcgccacg gcagccggga ca
//
LOCUS       DAOQNQ010000172         6996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_4116_length_6996_cov_4.895692, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000172 DAOQNQ010000000
VERSION     DAOQNQ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6996
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4116_length_6996_cov_4.895692"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="PK754_10605"
     CDS             complement(<1..642)
                     /locus_tag="PK754_10605"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNB09999.1"
                     /translation="MTFRFFTFLICIGVYSIAYTSANAQTGASGRVEKFRALYEDSSG
                     VWIDSLPTAEDSVFIKNKKYYFTGVNSRGKETGLDAFDDLTLNLSLTVGEEEERQLGW
                     FTRAVSWTGNNLIRILSLTFYDPEWGANRYFFNAETFANDLKVINQLYNDKGYFDATI
                     VKYTAKFDKHRESIEITIYIYEGEPTTLAEDPDIHIQSPIPVADPGSKFQRKKI"
     gene            complement(649..990)
                     /locus_tag="PK754_10610"
     CDS             complement(649..990)
                     /locus_tag="PK754_10610"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNB10000.1"
                     /translation="MFIGRVVGNVWATKKVENVKSLRFLMVHPVNIDKDPNTNVVVVA
                     DTLGAGIGELVLVSYGHAARRAITEAPNELSIEAAVIGIVDEIEVDETLLRGLNTAKL
                     KSTFLPDKQSR"
     gene            complement(1019..2521)
                     /locus_tag="PK754_10615"
     CDS             complement(1019..2521)
                     /locus_tag="PK754_10615"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HNB10001.1"
                     /translation="MDSIFWQVTLSDGIGYALAFLSLGVILATVELLRRRFFWPPEIT
                     RKITHIGVGILIFFAPSWFDSGAPAVYIGVFFVVFNMLAIRGHWLQGIHGTDRRSYGT
                     VYYPMAFTMLVLLCWETAPLVLMSAMLVLALGDASAGLVGQLWPSPRHYYLTSDRKSI
                     EGSITMFVVSALVIAFSFLYYQNAHIAEFSKFTIICAALIGALLATIMEAISPAGSDN
                     LTIPLTVAWVVYTITHQTEEATVYLIYGVGLAACVGWASFRFRLLTADGAAGAFLLGS
                     VIFGIGGWMWALPILVFFISSSLLSRLGKRRKAQFDTIFEKGSTRDAGQVLANGGLAG
                     AVALAAQLYPGEVWYIVYLAVIAAATADTWATEIGTFFQATPRSVLHWRKVQPGTSGG
                     ISLPGTLGAFTGSLWIAMTGAYVMGEYLNTQGLWFVTLAGFLGSIADSFLGATLQAQY
                     ACPTCGKPTEKKQHCDEPTRQVRGLVWMHNDAVNMIASGFGGILAYIMMT"
     gene            complement(2687..3376)
                     /locus_tag="PK754_10620"
     CDS             complement(2687..3376)
                     /locus_tag="PK754_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10002.1"
                     /translation="MKPIRSWIVLIFVLMSGWSLQGQNEEQTDETNSNRKKIGIGISF
                     GTNTHRFGDDDDYGYFKTGAIMGIYVPINFGDRWRIEPEVSYNNMSGKISRSDDDYYY
                     KSNSTVKISRYGVGILRRKKRDHTETYFGARLGKVFIEDKTRFTYSAPGYEDGDTDKR
                     HKSDLYFGPVAGGEYYFSPYFSFGGEAQLIYTKVGRMYGDEYEDDPDEDISYNVHAWD
                     TRAHLFVRWYF"
     gene            complement(3423..4562)
                     /locus_tag="PK754_10625"
     CDS             complement(3423..4562)
                     /locus_tag="PK754_10625"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HNB10003.1"
                     /translation="MGFSTDAIHAGQIPDPTTGAVITPIYQTSTYAQEELGVSKGFDY
                     GRTINPTRVALEANIAALEKGKYGFAFGSGMAAITNIAALLNGGDHVIVSNNVYGGTF
                     RFFDKIMKRFGLEFSWVDTSDEKNIRAAIKPNTKMLYVETPTNPMLTITDLQVVAALA
                     KEHKLISVVDNTFMSPYFQTPLEFGVDIVLHSTTKYINGHSDVIGGVVVTSSEELKEK
                     LKFLQNSIGAVPSPFDCWLVLRSTKTLALRMRQHNENAIAMAEFLAKHPKVKKIYYPG
                     LASHPQHALAKKQMRGFGGMISIELGSMDAGKNMLRNVKIFALAESLGGVESLICHPA
                     SMTHGAIPLEDRLKLGLTDGLVRLSVGCEDKEDLIADIENALKAV"
     gene            complement(4588..5943)
                     /locus_tag="PK754_10630"
     CDS             complement(4588..5943)
                     /locus_tag="PK754_10630"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR01137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HNB10004.1"
                     /translation="MKRIYNNILEVIGQTPVVKLNRIGAQLQCNLYAKCEFMNPGGSI
                     KDRIGRAMVEAAEREGRIKPGDTLIEPTSGNTGIGIALAGAIKGYRVIITMPEKMSRE
                     KQVILEALGAEIIRTPTEAASEDPESHISVAQRLQRELPNAHILDQYSNPNNPKIHYD
                     TTGQEILDALDGKVDMVVVAAGTGGSITGIAKRMKDANPQCIVVGADPVGSILGGGTH
                     VGTYKVEGIGYDFIPKVLDRDCVDEWIKTEDQSSFLLARRLIHEEGLLVGGSSGSSLF
                     AALQAAQRLKPGQNCVVVLSDGVRNYMTKFVDDKWMRDNGFFENKSIQGTVKDILAKN
                     PARPVLICADDMESVRQTISKMREKGVSQLPVTSGGALVGIVSESDIMDYLASGAGTQ
                     EAKVSKCMTRQVRMVGAQTPISTVQENLRQSNAVVVVDDSKRPISIMTKIDLLDYLTT
                     K"
     gene            complement(5958..6365)
                     /locus_tag="PK754_10635"
     CDS             complement(5958..6365)
                     /locus_tag="PK754_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10005.1"
                     /translation="MIASHDQYFGEIIRTVEAIAPQTLVAVISWEGLVLRSNVSDEHV
                     EGQFGALASVFLDQGKRMFTIPFDGKKPQPDTVQTMVTVGTKHVIIMTQLLPGILLAV
                     SSGDKSKINTFLTCSLEEVDKLHAMIAKKEIIF"
     gene            complement(6372..6722)
                     /locus_tag="PK754_10640"
     CDS             complement(6372..6722)
                     /locus_tag="PK754_10640"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNB10006.1"
                     /translation="MEDILKRLRNDTPGVSAVALVSSDGLVISSMLPDTIEAERVAAI
                     SAALLNQGESSSEDAMIGEMAQVTIRAKEGFIIITRAAKETLLTVFTTTQAKLGLVLF
                     DISVAIKELQPHIH"
     gene            complement(6794..>6996)
                     /locus_tag="PK754_10645"
     CDS             complement(6794..>6996)
                     /locus_tag="PK754_10645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNB10007.1"
                     /translation="PDPSKPVKPVAVKGDIADSSRPSKGCLFQARCPWVEAVCRDETP
                     RWRSCGQERFVRCHKVTGDNPH"
BASE COUNT         1841 a         1804 c         1568 g         1783 t
ORIGIN      
        1 tattttttta cgctgaaatt tggaacccgg atcggcgacg ggaatcgggc tttgaatatg
       61 aatatcagga tcctcggcca acgttgtcgg ttcgccttcg taaatataaa tcgttatctc
      121 aatgctttcg cgatgcttgt caaatttggc tgtatatttt acgatcgtcg cgtcaaaata
      181 acctttgtcg ttgtatagtt gattgatgac ttttaagtca ttggcaaacg tctcggcatt
      241 aaaaaaatac cgattggcgc cccactccgg atcataaaat gtcagcgaca aaatacgaat
      301 gaggttattc cccgtccagc tcaccgcgcg cgtaaaccat ccgagctgac gttcttcctc
      361 ttcgccgacc gtaagactga ggtttaatgt aagatcatca aaggcatcta aaccggtttc
      421 tttaccccgg ctattgacac ccgtaaaata atattttttg ttttttataa atacgctgtc
      481 ctccgccgta ggcaaactat caatccaaac gcctgacgaa tcttcgtata acgcgcgaaa
      541 tttttccaca cgtccggaag cgccggtctg tgcattggcc gaggtgtacg cgatcgaata
      601 aacaccgata caaataagga atgtaaagaa acggaaggtc ataagaagtt atctggattg
      661 tttgtccgga agaaacgtag atttgagttt agccgtattg agtccgcgca acaacgtctc
      721 gtccacttcg atttcatcca cgatgccgat taccgccgct tcgatggaga gttcgttggg
      781 cgcttcggtg atggcacgac gggcggcatg gccgtatgaa accaacacca attcaccgat
      841 gccggccccc agcgtatcgg cgacgaccac aacattggtg ttcggatctt tatcaatatt
      901 tacgggatgc accatcagaa aacgtaatga ctttacattt tctacttttt tagtcgccca
      961 aacattacct accacgcgtc cgatgaacat gcgtcaatcc gttgattaat gaaaattttt
     1021 aagtcatcat tatataagcc aatattccgc cgaaaccgct tgcgatcata ttcacggcat
     1081 cattgtgcat ccataccaaa ccgcgcactt gacgtgtggg ttcatcacaa tgttgttttt
     1141 tctctgtagg ctttccgcac gttgggcaag cgtactgggc ctgcagcgtc gcacccaaaa
     1201 aactgtcggc tatacttccc aaaaaacctg ccaacgtaac aaaccacagg ccttgtgtat
     1261 tcaggtattc acccataacg tatgccccgg tcatcgcgat ccacagcgaa ccggtgaaag
     1321 cgcccagcgt accaggcaat gaaattccgc cggaagttcc gggttgtact ttacgccaat
     1381 gtaaaacact gcgcggggtc gcctgaaaaa aagtaccgat ttctgtagcc caggtatccg
     1441 ccgtcgctgc agcgatcacg gccaaataaa cgatatacca gacttcaccg ggatataact
     1501 gagcggctaa tgcaacggcc ccggccaagc cgccattggc cagtacctgt ccggcatcac
     1561 gggtactgcc tttttcaaag atcgtatcaa actgcgcttt acggcgtttg cccaagcgcg
     1621 acaataaact ggatgatata aaaaacacaa gaatcggtaa agcccacatc catccgccga
     1681 ttccaaaaat cacactgccc aacagaaacg cacctgccgc gccgtcggcc gtcaataagc
     1741 gaaagcgaaa cgatgcccag cctacgcaag ctgccaaacc cacgccgtat atcaaatata
     1801 cggttgcttc ttccgtttga tgcgtgatcg tatatacgac ccacgccacc gtcagcggaa
     1861 tagtcagatt atccgagccg gcgggcgata tggcttccat gatcgtcgcc aaaagcgctc
     1921 cgatcaatgc ggcacagatg atcgtaaatt tcgagaattc cgcaatgtga gcattttgat
     1981 aatacaaaaa agaaaaagca atcacaagcg ctgatacgac aaacatagtg atcgaacctt
     2041 cgatgctttt tcggtcggag gtcagataat aatgacgcgg tgaaggccat aattgcccta
     2101 ctaagccggc ggatgcatca ccgagcgcta aaaccaacat ggctgacatt aaaactaacg
     2161 gggccgtctc ccaacacagc agtaccaaca tcgtgaaagc catcggataa tatacggtcc
     2221 catacgaccg gcgatccgtt ccgtgaattc cctgcaacca atgcccgcga atcgcgagca
     2281 tattaaacac aacaaaaaaa acgccgatat acacggccgg cgctcccgaa tcgaaccagg
     2341 aaggcgcaaa aaaaatcaga atgccgacgc caatatgggt tattttacgc gtgatttccg
     2401 gtggccaaaa aaaccgacga cgtaataatt ccaccgtcgc caagatgaca cctaacgaca
     2461 aaaaggccag tgcatagccg atcccatcac tcagagtcac ttgccaaaaa attgaatcca
     2521 taatcttcta aacagggaga ggataaatgc gtttagtatc aatacgctaa gacgtgataa
     2581 taaccgattg ggtcttatta aaaacttcta accgaattcg ttcgtactac taggaatgca
     2641 aacctattaa ttgtatgcgc agtgaagacg gcatgtttat tgaaatttaa aaataccagc
     2701 gtacaaataa atgcgcacgg gtatcccatg catggacatt ataactgata tcttcgtccg
     2761 gatcatcttc gtattcatca ccgtacatac gaccaacttt agtatagatc aactgtgctt
     2821 cgccgccaaa actgaaatac ggtgaaaaat aatactcacc gccggccacc ggtccaaaat
     2881 acaaatctga tttgtgtctt ttatcggtat caccgtcttc atatccgggc gccgaatagg
     2941 tgaatcgtgt tttatcttcg atgaacactt tgcccaaacg tgcgccaaaa taagtttccg
     3001 tatgatcacg tttttttcga cgtaaaatcc caacgccata gcgggatatt tttaccgttg
     3061 agttagactt gtaataataa tcatcatcgc ttctactgat tttaccggac atgttgttat
     3121 aactcacttc cggttcgata cgccatcggt caccaaaatt gatcggcaca taaatcccca
     3181 taatggcgcc ggttttgaaa taaccataat catcgtcatc gccaaaccgg tgtgtattcg
     3241 tgccgaacga aattccgatc ccaatttttt ttcggttgga attagtttcg tctgtttgct
     3301 cttcgttttg accttggagc gaccatccgc tcatcaatac aaaaatcaaa acgatccagc
     3361 tgcgtatcgg tttcatttat tctccccttt tttaatacag aatagagccc ccgtaagaaa
     3421 aactacacgg ccttcaacgc attttcgata tccgcgatca aatcttcttt gtcttcgcaa
     3481 cccacggaca aacggaccaa accgtcggtc aaaccgagtt tcaaacggtc ttccaacgga
     3541 atcgctccat gtgtcatcga agccggatgg catatcaggc tttccacacc gccgaggctt
     3601 tcggcgaggg caaatatttt tacgttacgc agcatgtttt tgccggcatc catcgaaccc
     3661 agttcgatcg aaatcatccc gccaaaacca cgcatctgtt ttttggccag cgcatgctgc
     3721 ggatgcgatg ccaaaccggg gtaatagatt ttttttactt taggatgctt agccaaaaac
     3781 tccgccatag ctattgcatt ttcattgtgc tgtcgcatac gcaacgccag agttttcgtc
     3841 gaacgcagaa caagccaaca atcaaacggc gacggtacgg cgccgataga gttttgtaaa
     3901 aatttgagtt tttcttttaa ctcttcgctg gaagtgacca ccacaccgcc gatcacatcg
     3961 ctgtggccgt tgatatattt ggtcgtactg tgcaaaacga tgtccacacc aaactcaagc
     4021 ggagtctgga aatacggact cataaatgta ttatctacga cggaaatcag tttgtgttct
     4081 ttggcgagtg ccgcaacgac ctgcaaatcc gttatcgtca gcatcggatt ggtcggcgtc
     4141 tcgacataca acattttggt gttaggcttg atagccgcac ggatattttt ttcgtccgac
     4201 gtatccaccc aggaaaattc aagaccaaaa cgcttcatga ttttgtcaaa aaaacgaaat
     4261 gtaccgccgt acacattatt ggaaacgatg acatgatcac cgccgttgag cagtgcggcg
     4321 atattggtaa tagcggccat accgctgcca aaagcgaaac cgtatttgcc tttttcaagc
     4381 gccgcgatat tcgcttccaa cgcgacgcgg gtgggattga tcgtgcgccc gtaatcaaaa
     4441 cctttactga cgccaagctc ttcctgagcg tacgtcgaag tttgataaat cggcgtgatc
     4501 accgcaccgg tcgtcggatc cgggatctgg ccggcgtgaa tggcatccgt tgaaaaaccc
     4561 atatttattc tttcctacta tgcgttatta tttggttgtc agataatcca gcaaatcaat
     4621 ttttgtcata atgctgatcg ggcgtttgct atcatccacg accaccacgg cgttggattg
     4681 gcgcagattt tcctgcactg tagatatcgg cgtttgcgca ccgaccatac gcacctgacg
     4741 cgtcatgcat ttggaaactt tggcttcttg ggtaccggca ccgctggcca aataatccat
     4801 gatatcgctt tccgatacga tcccgaccaa tgcgccgccg gaagtaaccg gcaattgcga
     4861 aacacctttt tcgcgcatct tggaaatcgt ctgacgcacg ctctccatat catcggcaca
     4921 gatcagcacg gggcgtgccg gatttttagc tagaatatct ttgaccgtac cctgaatgga
     4981 tttgttttca aaaaatccgt tatcgcgcat ccacttgtcg tccacaaatt tggtcatata
     5041 gttacgtacg ccgtccgata aaacgaccac gcaattttgt ccgggcttta accgttgtgc
     5101 ggcctgcagt gcggcaaaaa gtgatgaacc ggacgaaccg ccgacgagca aaccttcttc
     5161 gtgaatcagg cggcgcgcca ataaaaatga ggactgatct tccgttttga tccattcatc
     5221 cacgcaatcc cgatcgagta ctttgggaat aaaatcatag ccgatacctt cgaccttgta
     5281 tgtaccgacg tgcgtaccgc cgccgaggat cgaacctacc ggatcggcgc cgacgacaat
     5341 acactgcgga ttggcatctt tcatacgttt ggctatgccc gtgatggagc cgcccgtacc
     5401 ggcggcaacg acgaccatat ctactttgcc atccagtgcg tcgaggattt cctgaccggt
     5461 cgtatcgtag tgaattttgg gattattggg gttggagtat tgatctaaaa tatgtgcatt
     5521 cggtaactcg cgttgcaggc gttgcgcgac actgatatga ctttccggat cttcggaggc
     5581 cgcttcggtc ggtgtgcgaa taatttcggc gcccagcgct tcgagtatta cttgtttttc
     5641 gcggctcatt ttttcgggca tcgtgatgat cacacgatac cctttgatcg cgccggccaa
     5701 tgcaataccg ataccggtat taccgcttgt cggttcgatc aacgtatctc ccggcttgat
     5761 acgaccttcg cgttccgccg cttcgaccat tgcgcggccg atacggtctt tgatggaacc
     5821 gccgggattc ataaattcac atttggcgta cagattacat tgaagctgtg ctccgattcg
     5881 attgagtttg acgaccggtg tttgaccgat gacttcgaga atgttgttgt atatccgttt
     5941 cataattcct gtagatatta aaaaatgatt tctttttttg cgatcatcgc gtgcagtttg
     6001 tccacttctt cgagcgaaca ggtaaggaac gtattgattt tggatttatc accggaggaa
     6061 acagccaata gaatccccgg taaaagttgg gtcataatga tgacgtgttt cgtaccgacc
     6121 gtgaccatcg tctgcacggt gtccggctgc ggtttttttc cgtcaaaagg aatagtgaac
     6181 atacgtttgc cctgatctaa aaaaacggag gctaaagcgc caaactggcc ttcgacgtgt
     6241 tcatcggata cattggaccg caaaactaat ccttcccagg aaatcacggc caccaaggtt
     6301 tgcggggcaa tcgcctcaac ggtacgaatg atttccccaa aatactgatc gtgcgatgct
     6361 atcatgatgc ctcagtgaat gtggggttga agttctttga tggcgacgct gatgtcaaac
     6421 aagacaagcc cgagtttggc ttgggttgtg gtaaagacgg tcagcagcgt ttctttggct
     6481 gcccgcgtga tgatgataaa tccttctttg gcgcgtatcg tgacttgcgc catttcaccg
     6541 atcatcgcgt cttcggaact gctttcgccc tgattcagca acgcggccga aatcgcggcc
     6601 acgcgttcgg cttcgatggt gtccgggagc atggacgaga tgacgagccc gtcgctactg
     6661 acgagcgcaa ccgcgcttac acccggcgta tcattgcgca ggcgtttgag aatatcctcc
     6721 atgatgcacc taaaaatcgt tggaaaaagt gagaaattat tcgccgcaaa tatcataaat
     6781 agttaggcca aagtcaatgc ggattgtccc ctgtaacttt atggcagcgt acaaaacgct
     6841 cctgcccgca agaacgccag cgcggcgttt catcacggca aacggcctcg acccacggac
     6901 aacgcgcctg aaaaaggcaa cccttggaag ggcgcgacga atccgcgata tcacctttta
     6961 ctgcgacggg ttttaccggc ttggacggat cgggtt
//
LOCUS       DAOQNQ010000173         6982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_4124_length_6982_cov_6.259997, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000173 DAOQNQ010000000
VERSION     DAOQNQ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6982
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4124_length_6982_cov_6.259997"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PK754_10650"
     CDS             <1..299
                     /locus_tag="PK754_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNB10008.1"
                     /translation="KPVYEDSDVIAFHDLHPKAPTHILLIPKKHIEKLSDMTGDDSEL
                     GGKLLLAVGKVAEAAGIKDNGFRVVVNNGKYAGQEVFHLHLHILGGKPMGWNPA"
     gene            352..963
                     /locus_tag="PK754_10655"
     CDS             352..963
                     /locus_tag="PK754_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10009.1"
                     /translation="MKLFIMFCILFTFIGVAMVTAQQPQGEFHKYIIKKTDDERRKTK
                     GQEWWGAGAVVFSKEEIPRLGKTAEVQPLLQTQFNHGDTFFGRVYSPVHMGGLPGGMP
                     EVIEYRVIENGKRIYEIESRGDALPESDWSSWLMELPSDLQRCFDELPEGTHNLRVEV
                     WSARTVEVLTEWKDRETDKTVGFTKENEGRIKFIAAGDIVYVK"
     gene            1080..2042
                     /gene="lipA"
                     /locus_tag="PK754_10660"
     CDS             1080..2042
                     /gene="lipA"
                     /locus_tag="PK754_10660"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNB10010.1"
                     /translation="MSRQYPMTSVQVDYIKKKTEEVQEKEPLKRPEWLKVKIPIGEKF
                     SEIKNLVGSKKLHTVCEEARCPNLSECWNRGTATFMILGDTCTRSCGFCAVKTGKPTW
                     LDKDEPRRVAESVRTMNLRHAVITSVNRDELADGGSMIFAETIRAIRKAIPTCRIEVL
                     IPDFKGNFENLKNVIDAHPDILNHNTETVPRLYQVVRPQANYAQTLEVLDRSKKNGMI
                     TKSGLMLGLGEKNDEVLAVMDDLRKVDLDILTLGQYLQPTKEHIPVDRFVHPDDFAMF
                     REKGMAMGFRVVESGPLVRSSYHADEQTDVVADSFVRRDSVQIL"
     gene            2141..3859
                     /locus_tag="PK754_10665"
     CDS             2141..3859
                     /locus_tag="PK754_10665"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNB10011.1"
                     /translation="MSTFKEYSLSPEVQKAIEEMGFVTPSEIQSKAIPLLLSHHGDFV
                     GQAQTGTGKTAAFGIPLIEKIDPSAKTVQAIILSPTRELAVQISEELKKLSKHKRLRI
                     LPIYGGQDIGVQLRALRQGVQIVVGTPGRVVDHLHRGTLQLSQIKHFVLDEADEMLDM
                     GFLEEIEEILTHAGTERSIWLFSATLSGQIRGIAKRFMTDPREVKIQKNTATTENTEE
                     IYYAVQEHNKFEALCRLIDHAKEMYAIVFCQTKAQCAEVAERLVLKGFKAEALHGDMN
                     QAQREHVMRKFKAKGVRLLIATDVAARGIDVNDLTHVINYSLPMESESYIHRIGRTGR
                     AGKTGIAITLVTPSENYKLRRLEGNTRKRITKSTIPGIQEIMEANIDRTITEFEEALK
                     NPAGFDRYFDRWKPIIKKYETEEIMRGLVTLLCREFLEKYEDDKELNVPDNFGQRSGM
                     RDRSYAPRAEMTELRINVGAQHNLQTGTLVGKICNMARLEGRQVGKVFINEDHSVFRV
                     DAESVDRVIRSLTGITIGGKKAVVERTDGRSGGAPRMPFAKRSSHAKEDSPFRKSRTY
                     KKDHSS"
     gene            3884..4366
                     /locus_tag="PK754_10670"
     CDS             3884..4366
                     /locus_tag="PK754_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HNB10012.1"
                     /translation="MKRFMILGLLMVVGLSTAFAGDPDAIIGKWYNEEGTAQIEIYKQ
                     GSQYFGKIVWLKEPTYNANDVNDNEGEPLVKLGAEKVDFKNPDSGRRSQKIIGLVILR
                     DFTYDSAEGEWSNGMIYDPKKGSDYKCYMQLMEQNKLKIRGFIGFSLIGRTSYWSRAN
                     "
     gene            complement(4670..>6982)
                     /locus_tag="PK754_10675"
     CDS             complement(4670..>6982)
                     /locus_tag="PK754_10675"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HNB10013.1"
                     /translation="GGATDTKQTTLTVKNINRPPMFDALAASFTTDENKPVSFTIVAS
                     DLDKEDAGKLEWGGFNIPPGATFDRSSGVLTWTPSYDQSGKYDGIYFIVRDAEGLSDT
                     VNTSITVNHVNRPPFFATVDAQKIDEVKPLSFKIAAADADAEDDGKLTIKATTVPQGA
                     IYNEITRTLTWTPTYDQSGDYTAVFKVTDPAGASDEMTVPVQVVNINRAPSIAAVPAQ
                     NADENKAYTLVVTEGTDPDKEDVGKLTYKAENLPQGATFDPATRTIAWTPTYDQSGVY
                     EGVKVTVTDALGLTATTTFRFTVKNINRPPVMEVIAAQTSDEQKPLTFQVKFSDPDKE
                     DANRLTVAAAGMPLGASFAGGNFTWTPTFDQAGNYTVKFTVTDFEKLSDTKEAVITIN
                     NVNRPPVLAAILAQAATENTPFTFTVPAANDPDKEDAGKLTYKAEGLPEGANFDAATR
                     VVKWTPTFDQSGDHNIRITVTDVLGVADNKELVIKVKNVNRTPVVTDVAAQNGDENAK
                     MEFRVETSDPDKEDADKLKVVAEQMPQGATFANNTFSWTPTFDQAGTYRVTFRATDVA
                     GGFAVKTAVITIKNVNRNPVLGAINNQTADENKFMSFTVSASDPDKEDQIRLNVTASD
                     LPKGATYAAGKFSWMPGYDQAGDYTVKFTAKDPNGGEDVKSVSIKVNNTNRAPKMNAV
                     GTKNGKEGSEISFSASASDPDNDNLSFSLVGGPSGATMSSDGKFTWTPQTGQSGTFNF
                     TVKVSDGTAEASAPVTVIVAKAPQPQGGGQ"
BASE COUNT         1848 a         1647 c         1626 g         1861 t
ORIGIN      
        1 ccaaacctgt atatgaagac agcgatgtta tcgcgtttca tgatctgcat cctaaagcgc
       61 cgactcatat tttattgata cccaaaaaac atatcgaaaa attatccgac atgacgggcg
      121 atgattcgga gctcggcgga aaattattac ttgccgtagg caaagtagcc gaagcggccg
      181 gcatcaaaga caatggtttt cgcgtcgtcg taaataatgg taaatacgcc ggccaagaag
      241 tttttcatct tcatttgcat atccttggcg gcaaaccgat gggctggaac cctgcctgat
      301 tcttttgtcc tttctgctta tgcgttaaat aaaaaaaaac ggagcatccg tatgaaactt
      361 tttatcatgt tttgcatcct atttactttc ataggagtgg caatggtcac agcacagcag
      421 ccgcaaggtg agttccacaa atatattatc aaaaaaacgg atgatgaacg ccgtaagacc
      481 aaagggcagg aatggtgggg cgccggcgcc gtggtttttt ccaaagaaga aatcccgcgt
      541 ctcggtaaaa ccgcagaggt gcaaccctta ttgcaaactc aatttaatca cggcgacact
      601 ttttttgggc gcgtatatag ccccgtacac atgggcggcc tgccgggcgg aatgccggaa
      661 gtaatcgaat accgtgtcat cgaaaacggt aaacgcattt atgaaatcga atcgcgcggt
      721 gatgccctgc cggagtcaga ttggtcatct tggcttatgg aacttccaag tgatttgcaa
      781 cgctgttttg atgaattgcc cgagggtacg cacaatctcc gcgtcgaagt gtggagcgct
      841 cgcaccgtag aggttctgac tgaatggaaa gaccgcgaaa ccgataaaac cgtgggtttt
      901 accaaagaaa atgaagggcg gatcaagttt atcgccgccg gtgatatcgt gtacgtcaaa
      961 tgagttaaca acgacgaata atgcaggaat acccgagatt aaaatgattc attttccctt
     1021 tgcttttggc gtcgggattt gatataacgt ctcgaaactg agaagtaaga aagttcgcta
     1081 tgtcccgtca atatccgatg acttccgtac aggttgacta catcaaaaag aaaaccgaag
     1141 aagttcagga aaaagaaccg ctcaagcgcc ccgaatggct caaagtcaaa attccgatcg
     1201 gtgaaaaatt ttcggaaatc aaaaaccttg tcggttccaa aaaactgcat accgtctgcg
     1261 aagaagcccg ttgccctaat ctgtccgaat gttggaatcg cggcacggcc acgtttatga
     1321 tactcggcga tacctgtacc cgcagttgcg gtttttgtgc ggtcaaaaca ggtaaaccga
     1381 catggctcga taaagacgaa ccccggcgcg ttgccgagtc cgtgcgaacg atgaatcttc
     1441 gtcatgccgt aatcacgtcc gtcaatcggg atgaactggc ggacggcggt tctatgattt
     1501 ttgccgaaac gattcgcgca attcgtaaag ccattcccac atgccgcatc gaagtactga
     1561 tccctgattt taagggcaat tttgaaaatc tcaaaaacgt cattgatgcg caccctgata
     1621 tactcaatca caatacggaa actgttccgc gtttgtatca ggtcgtacgc ccccaggcca
     1681 actatgcaca aacgttggag gtgcttgatc gctccaagaa aaacggaatg atcaccaaaa
     1741 gcggactcat gctcggtctt ggcgaaaaaa acgatgaagt acttgccgtc atggacgatc
     1801 tgcgaaaagt ggatttggac atactcacac tcggccaata tctccagcct accaaagagc
     1861 atatccccgt ggatcgtttt gttcatcccg atgattttgc catgttccgc gaaaaaggca
     1921 tggctatggg ttttcgtgtg gtggagtccg ggccgctggt acggagttcg tatcacgccg
     1981 atgaacaaac cgatgtggtg gccgattctt ttgttcgccg cgacagcgtg caaattttat
     2041 aaacctgtct tttatgattt ttatgaagac cgctgatatt tcataaaacg tcatagtcat
     2101 aatttattga tttctcatgt cagcgtttcc caaggaaagt atgtccacat tcaaagagta
     2161 ttctcttagt cccgaagtgc aaaaagccat cgaagaaatg ggttttgtca caccgtctga
     2221 aattcaatca aaagccattc ctctgctttt atcacaccat ggcgattttg tcggccaggc
     2281 acaaaccggt accggtaaaa ccgcggcttt tggcattccg ctcatcgaaa aaattgatcc
     2341 ctccgctaaa acggtacagg ctattattct ttccccgaca cgcgagttag ccgtgcaaat
     2401 cagcgaggaa ttaaaaaaat taagtaaaca taaacgcctg cgtattcttc cgatttacgg
     2461 gggtcaagat atcggcgtac aactccgtgc gttgcgccaa ggtgtgcaga tcgtcgtcgg
     2521 tacgcccggt cgcgtcgtag atcatttgca tcgcggcaca ttacagcttt cgcagatcaa
     2581 acactttgtc ctcgatgaag ccgacgaaat gcttgacatg ggttttcttg aggaaatcga
     2641 agaaattctt acgcatgcgg gcaccgaacg cagcatctgg cttttttcgg ctacgctcag
     2701 cggacaaatt cgcggtattg ccaaacgatt tatgaccgat ccgcgtgaag tgaaaattca
     2761 aaaaaacacg gccacgacgg aaaacaccga agagatttat tacgccgtgc aggaacacaa
     2821 taagtttgaa gcgttatgcc ggcttattga tcacgccaaa gaaatgtacg cgatcgtttt
     2881 ttgtcagacc aaagctcagt gcgccgaagt cgccgagcgc ttggtgctca aaggatttaa
     2941 agccgaggct ttgcacggtg acatgaatca ggcccaacgc gagcatgtga tgcgtaaatt
     3001 taaagctaaa ggcgtgcgcc tgttgatcgc gacggacgtt gccgcgcgtg gcattgacgt
     3061 caatgatctt acgcacgtca tcaattattc gctgccgatg gaatccgaaa gctatattca
     3121 ccgcatcggt cgtaccggac gtgccggcaa aacaggaatt gccattaccc tggtaactcc
     3181 gtcggaaaat tataagcttc gtcgccttga aggcaatact cgtaaacgta ttaccaagtc
     3241 cacgattccc ggcatacaag aaatcatgga agccaatatt gaccggacga ttacagaatt
     3301 tgaagaggct ttgaaaaatc cagccgggtt tgatcgttat tttgaccggt ggaaacccat
     3361 aatcaaaaaa tatgaaaccg aagaaatcat gcgcggtttg gtaaccttgc tttgccgtga
     3421 atttttggaa aaatacgaag acgacaaaga acttaatgta ccggataatt tcgggcagcg
     3481 tagcggaatg cgtgaccgca gctatgcacc gcgtgcagag atgaccgaac tgcgtatcaa
     3541 tgtcggcgct cagcacaatc tgcaaaccgg tacgctcgtc ggaaaaatat gtaatatggc
     3601 gcgccttgaa ggacgacagg tgggaaaagt atttatcaat gaagatcatt ccgtgttccg
     3661 cgtggatgcc gaatccgtgg atcgtgtgat ccgttccctc acaggcataa ccatcggcgg
     3721 aaaaaaagcc gtcgtcgaac ggacggacgg acgctccggc ggcgcaccgc gtatgccttt
     3781 cgccaaacga tcatctcatg caaaagagga ttccccgttt cgtaaatcac gaacatataa
     3841 gaaagatcat tcttcttaat tcatcattca aaggaggcgc tgtatgaagc gttttatgat
     3901 cctcggttta cttatggtag tcggtttgtc aaccgcattt gcgggcgatc cggatgccat
     3961 catcggtaag tggtacaatg aagaaggcac ggcacaaatc gaaatttaca aacagggttc
     4021 acagtatttt ggcaaaatcg tatggctcaa agaacctacg tataacgcca atgatgtaaa
     4081 cgataatgaa ggcgaaccct tagtcaaact tggcgccgaa aaagtagatt tcaaaaaccc
     4141 cgattcgggt cgtcgctcac aaaaaatcat cggtttggtg attttgcggg acttcacata
     4201 tgactccgct gaaggtgaat ggagcaacgg tatgatctat gatcccaaaa aaggttctga
     4261 ttataaatgc tatatgcagc tcatggaaca gaataaattg aaaatccgcg gattcatagg
     4321 gttttcattg atcgggcgca cttcgtattg gagtcgtgcc aattaattag gaattacagt
     4381 ttatttgttt gatcgtaaaa acccgctaat gtctttagcg ggttttttta tttgtaccaa
     4441 cagtatgaca cttattaaga aacgtaagag tatggtataa gccgtaagcg ttatacaatg
     4501 actattctat tcacatgcgt tacattcagg tgtagctctt gctgttgaat ttgatatcag
     4561 aatactttct attcgatcat ttctatctac acatacggat tattgcataa aacaacaaag
     4621 ccctttgttt taccaaaggg ctttgatttt ttcagatcat gtttaaagct tattgaccgc
     4681 cgccttgcgg ttgtggtgct ttggcaacaa ttacggtaac cggagcagac gcttcggccg
     4741 taccgtcact tactttgact gtaaagttaa atgtgcccga ctgaccggtt tgcggtgtcc
     4801 acgtaaattt cccatcgctg ctcatagttg cacccgaagg gccgccaacg aggctgaacg
     4861 acagattatc attatccgga tcactggctg atgcactgaa tgaaatctca gatccttctt
     4921 tgccattttt tgtcccgaca gcattcattt tcggtgcgcg attagtatta ttcactttga
     4981 tggaaacgga ttttacatct tcaccgccgt tcggatcctt agccgtaaac ttcaccgtat
     5041 aatctccggc ttgatcatat ccgggcatcc atgaaaattt acccgccgca tacgtcgcgc
     5101 ctttcggaag gtccgaagcc gtaacattta agcgaatttg atcttcttta tcaggatcgc
     5161 tggccgacac tgtaaatgac ataaatttat tttcgtcggc cgtctgatta ttgatcgcac
     5221 cgagaacagg attgcgattt acatttttga tggtgatcac ggctgttttg acggcaaatc
     5281 ctccggccac atccgttgct cggaaagtca cgcggtaagt cccggcttga tcgaacgtcg
     5341 gcgtccagct aaaggtatta ttggcaaacg tagcgccttg cggcatttgc tccgccacta
     5401 ctttcagttt gtccgcatct tctttatccg gatcactggt ttcaacgcga aactccatct
     5461 tcgcattttc atcgccgttt tgtgcggcca catccgttac aacaggtgtg cggtttacat
     5521 tcttaacttt gattacgagt tctttgttat ctgcgacgcc caatacatcc gtaacagtaa
     5581 tgcgtatatt gtgatcgccg gattgatcaa acgtcggtgt ccacttgacg acacgtgttg
     5641 ccgcatcaaa atttgcacct tccggcaatc cttctgcttt atacgtgagc ttgccggcat
     5701 cttctttatc cgggtcgttc gctgcaggaa cggtaaatgt gaaaggagta ttttctgtcg
     5761 cggcttgcgc aaggatcgca gccaataccg gcggacgatt gacgttatta atcgttatga
     5821 ccgcttcttt ggtgtccgat aatttttcaa aatcagttac cgtaaattta accgtatagt
     5881 tacccgcttg atcaaacgtc ggtgtccacg taaaattacc gccggcaaag gaagcgccaa
     5941 gcggcattcc ggctgcagct accgtcaaac gattcgcatc ttctttatcc ggatcggaaa
     6001 atttgacctg gaaagtcaaa ggtttctgct catccgaagt ttgcgctgca atcacttcca
     6061 ttaccggagg acgattgata tttttgaccg tgaatcgaaa tgtcgtcgta gctgtaagtc
     6121 caagtgcatc ggtaacggtt actttgacgc cttcatatac gccggattga tcgtacgtcg
     6181 gtgtccatgc gatcgtacgc gtcgccggat cgaaagtcgc accttgcggt agattttcag
     6241 ctttgtaagt aagcttgcct acgtcttctt tatccggatc cgtaccttcc gttacgacca
     6301 atgtataggc tttattttca tccgcatttt gtgcaggaac tgcagcgata cttggcgcac
     6361 gattgatatt cacgacttga acaggtacag tcatttcatc gctggcaccg gccggatcgg
     6421 ttactttgaa tacagccgtg tagtcgccgg attgatcata ggtcggtgtc catgtcaaag
     6481 tgcgggttat ttcattatat attgcacctt gaggaacggt cgtcgctttg attgtcagct
     6541 tgccgtcgtc ttcggcatcc gcatccgccg ccgctatttt gaaagataac ggtttcactt
     6601 catcaatctt ttgcgcatcc acggtagcaa agaaaggcgg acgattgaca tggtttacgg
     6661 tgattgatgt attgacggtg tcgctcaatc cttcggcatc acgcacgata aaataaatcc
     6721 cgtcgtattt gcccgattgg tcatacgaag gcgtccacgt taaaacaccg ctgctgcggt
     6781 caaacgttgc gccgggagga atattgaatc caccccactc caactttccg gcgtcttctt
     6841 tatccaaatc gctggcgacg atcgtaaatg ataccggctt gttttcgtcc gtcgtgaacg
     6901 atgccgcaag cgcatcaaac ataggagggc gattgatgtt tttaaccgtc aacgttgttt
     6961 gttttgtatc cgtcgcgcca cc
//
LOCUS       DAOQNQ010000174         6953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_4145_length_6953_cov_6.316324, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000174 DAOQNQ010000000
VERSION     DAOQNQ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6953
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4145_length_6953_cov_6.316324"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /locus_tag="PK754_10680"
     CDS             <1..1419
                     /locus_tag="PK754_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNB10014.1"
                     /translation="LHGAVMVFLFIVPSIPAIIGNIILPLQIGAKDVAFPRLNLASWY
                     VYLAGSMFAIYSMITGGVDTGWTFYTPYSNTSNTSVISMTTAVLILGFSSIFTGLNFI
                     VTVHKLRAPGMTWFKMPLMVWGIYATALIQVLATPVLAITVVLLMLEKMWHIGIFDPA
                     MGGDPVLFQHFFWFYSHPAVYIMILPGMAIISELVAAFSHKNMFGYRFVAFSSLAIAL
                     LSFLVWGHHMFVSGQSELSAVIFSFLTFFVGVPSGIKIFNWLTTMYKGSISFSAPMLW
                     TFSFLFLFTIGGLTGIFLGTLSVDIHLHDTYFVVAHFHYVMMGGTVMALMGGLHYWWP
                     KIWGRMYNEFWARISVLLVFIGFNMTFFTQFVMGSRGMPRRYWTYQDQFTSLHGFSSI
                     GAFVIGAGFLIMAFYLIHSIFKGKPAGNNPWGALTLEWTTSSPPPHHNFDQTPIAVNG
                     PYDYDKVKAEITYEPNVPKAKV"
     gene            1444..2097
                     /locus_tag="PK754_10685"
     CDS             1444..2097
                     /locus_tag="PK754_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007280851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HNB10015.1"
                     /translation="MSSQHSAAHPAHLAHHFSDVEQQKESAKLGMWFFLLTEVLTFGG
                     LFCAYAIYRAWYPEMFSIAHQQLNVTMGTINTIVLITSSLTMALAIRNMQLNDKKGTL
                     IMLLLTFVFAGTFMVIKYFEYTHKIHLGQLPGKFYTFDGINASNPHIFFSIYFAMTGL
                     HGIHVMIGMILIAWLMYKTSKGAFSSEYYTPIEMTGLFWHLVDLIWIFLFPLFYLIG"
     gene            2102..2491
                     /locus_tag="PK754_10690"
     CDS             2102..2491
                     /locus_tag="PK754_10690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNB10016.1"
                     /translation="MSNEQHKEHIIPLSTYLGVFSALIALTALTVWIAQFHFGEWNLI
                     VAMSVAVVKALLVAFIFMHLLYDNKLYATVFAGSIVFLAVFIVFTMYDTLRRDDIYEQ
                     VGKPIKSKAIIYDNMPVKKDSTAHSGH"
     gene            2516..3463
                     /locus_tag="PK754_10695"
     CDS             2516..3463
                     /locus_tag="PK754_10695"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNB10017.1"
                     /translation="MNRFRQFALFSTIATYVLIFVGGLVRVSGAGLGCPDWPQCFGHW
                     FPPTSVDQIPPHIDPSLFNMTLAWIEYGNRIVGVTIGFLIAGTAWLAIKHYRHDKRIL
                     TASVAAAILVAFQGWQGSRVVKSQLNPHIISIHFALALLIISLMVYVTFRAYKPEQEI
                     KNISDAVYRSVFGWIKIMWVVNLLQILLGTEVRGALEVLANAFPLMSEAELMRQSNYV
                     SYIHGVVGVIIVGATWLFHEQIQRVRTQIPAYIVTISNIGLLLMIIQLFIGVLLVTMD
                     RPPLLQIVHLWISSFQIGLLVIFYAAWRRDNQSSERKTV"
     gene            3460..4050
                     /locus_tag="PK754_10700"
     CDS             3460..4050
                     /locus_tag="PK754_10700"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNB10018.1"
                     /translation="MNTNLIMRLSIGFFVLMLISAIVIYKAKESQSDLPVYAEVTPFA
                     MTERSGEAFTNEKFKGHITVVNFFFATCAGPCPAMNARVAELYRAFAGNNPVRFVSVT
                     IDPDRDSLVALRNYAMSFNVNDNRWSFLRGDLKSVEHLSENIFMVGSGAPTLHSTKLI
                     LVDHLGRIRGYYSSEEPASLTILKTHIRELVREMPS"
     gene            4047..4460
                     /locus_tag="PK754_10705"
     CDS             4047..4460
                     /locus_tag="PK754_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HNB10019.1"
                     /translation="MNAADLPFINAILNSVSTVFLTLGYIAIRKKDRVQHKRYMVTAL
                     ISSALFLTCYLIYHYQVGSVPYPYYDWTRPVYFVILVPHIILAAIMSPFIISAVHYAW
                     IGEFAKHKRLMRWVWPVWMFVSVSGVVIYLMLYRF"
     gene            complement(4446..6050)
                     /locus_tag="PK754_10710"
     CDS             complement(4446..6050)
                     /locus_tag="PK754_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10020.1"
                     /translation="MSTAPLQEERRSNGEIRSLVNILAQLAVESNKSSWHCIRETYAD
                     LLHLFHQNHEVRVSAPLLRTFEKNRRTLETVYNPTSVTGTHKQGPLREYWNKIIQMRP
                     KTNEKPVRKETDETVRGNYAQFLRQNVISLIEDQRILVLLLVGLLSRRLHHRSTSDIR
                     KTIQKFESDLKSHHARSDRAMSILIYSLCQTAMHTSLAADFAQGSLLCERAIELFRLL
                     PDYPRLRNELHLQLQEALRVSTERVVNRLQEKVLDEEIEIIEDLASKLSNPELVAYVM
                     PQLVFFLLRGQRYEECLSRMDGVLQTVTENKEFVDYISAITIGHYWCDALLKAERYSR
                     GEVNFSFIEREKDNFARVFDVVKTARVPDLGDETLYNVEIEKLAVSLSHLGTLYFQTG
                     NIPQASACYALSFENILLKKCSRNRDSVDEIMDRYKDTLFHPLNTAPPEAHPLIAMLA
                     AEKGFDFLRDTQAEKMKNEGKDAGEPIYYDDLINAQKELIADLMDATKNKIKHPFSAK
                     FSSYKPFVESEFARIVGKMKINLEAI"
     gene            complement(6140..6424)
                     /locus_tag="PK754_10715"
     CDS             complement(6140..6424)
                     /locus_tag="PK754_10715"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012195045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNB10021.1"
                     /translation="MSRKKLETYEIENRLKTLPDWKLRDGKLYREFIFSDFVEAFRFM
                     TAAALIAEKLNHHPDWSNVYNRVHITLYTHDVGGITELDFILADKLNTLL"
     gene            complement(6427..>6953)
                     /locus_tag="PK754_10720"
     CDS             complement(6427..>6953)
                     /locus_tag="PK754_10720"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNB10022.1"
                     /translation="HHSRHHKAYVDKLNDAVIQAKIDKPDLDTLMRHISKYSTAIRNN
                     GGGHWNHSMFWQIMKPGSGGAPSGELAAAINTAFGSFDEFKTKFNATAMSRFGSGWAW
                     LVVRDGKLEIGSTPNQDNPLMDVSDFKGTPLLALDVWEHAYYLKYQNKRADYVQAWWN
                     LIHWDEVTRRFKKL"
BASE COUNT         1705 a         1521 c         1624 g         2103 t
ORIGIN      
        1 ctgcacggcg ccgtgatggt gtttcttttt atcgtgccgt caattcccgc aattatcggt
       61 aatatcattc tgccgttgca gattggcgca aaagacgtgg cgttcccgcg tctcaattta
      121 gcaagttggt acgtatatct tgccggttcg atgtttgcta tttattcgat gattaccggc
      181 ggcgtggata cgggctggac attttataca ccgtatagca ataccagtaa tacgtctgtt
      241 atttctatga cgaccgccgt attgatcctt ggtttctcgt ccatttttac cggccttaat
      301 tttatcgtca cggtacacaa actgcgtgca ccgggcatga catggttcaa aatgcctttg
      361 atggtatggg gtatttatgc gacggcattg attcaggtac tggctacgcc ggtgcttgcg
      421 atcacggttg ttctcctcat gctggaaaaa atgtggcata tcggtatttt tgatccggct
      481 atggggggtg atccggtgtt atttcagcac ttcttctggt tctattcgca tccggccgtg
      541 tacattatga tcttgccggg tatggcgatc atttcggaac tggtggctgc gttctctcat
      601 aaaaatatgt tcggttatcg tttcgtagcg ttttcgtcat tggcgatagc gctcttgtct
      661 ttcctcgtat ggggacacca catgtttgtc agcgggcagt cggaattgtc agccgtgatt
      721 ttctcattcc ttacattttt cgtcggtgtg ccatcaggta ttaaaatatt caattggctt
      781 acgacgatgt ataaaggttc gatcagtttt tctgcgccga tgctgtggac attttcattt
      841 ttattcttat ttacgatcgg tggtcttacc ggtattttct tgggaacgtt gtcagtggat
      901 atacacttac atgatacgta cttcgtcgtg gcacattttc actacgtgat gatgggcggt
      961 acggtgatgg cgctgatggg cggcctgcac tactggtggc ccaaaatctg gggccgcatg
     1021 tacaatgaat tttgggcacg catttccgta ttgttggttt tcatcggctt caatatgacc
     1081 ttctttactc agtttgtcat gggttcccga ggcatgccgc gtcgttactg gacataccaa
     1141 gatcaattca cttcgttgca cggtttttcg tccatcggtg cgtttgtgat cggagcggga
     1201 tttttgatca tggcgtttta tttgattcat tccatattca aaggcaaacc ggcgggtaat
     1261 aacccttggg gcgctctgac attggaatgg accacgagtt caccgccgcc gcatcataat
     1321 tttgatcaga cgccgatcgc tgtcaatggc ccgtacgact atgacaaggt caaggcggaa
     1381 atcacatatg aaccgaacgt tcccaaagcg aaagtataat ttttttaacg ataacaggta
     1441 actatgtcaa gtcaacattc agccgctcat ccggcgcatc tggcgcatca ctttagtgat
     1501 gtcgagcagc aaaaagaatc cgccaaactc ggcatgtggt tcttcctgct gacggaagtt
     1561 ctgaccttcg gaggcctatt ttgtgcatac gcgatttatc gcgcgtggta tccggaaatg
     1621 ttttctattg ctcatcaaca actcaatgtc acgatgggta cgatcaatac gatcgttctg
     1681 atcacgagtt ccctcacgat ggcgttggcc attcgcaata tgcaactcaa tgacaaaaaa
     1741 ggtacgctca tcatgcttct gctgacgttc gtgtttgcag gaacatttat ggtgatcaag
     1801 tactttgagt acacgcataa aattcatctc ggtcaattac ccggaaaatt ttatacgttt
     1861 gacggcatca atgcatccaa tccgcacatt ttcttcagca tttattttgc gatgaccggg
     1921 ttgcacggta ttcacgtaat gatcggtatg attttgattg cctggctaat gtataaaacc
     1981 agtaaaggtg cgttttcatc ggaatattat acgccgatcg aaatgacggg ccttttttgg
     2041 caccttgtgg atttgatttg gattttcctt ttcccactct tttatttgat aggatgaggc
     2101 catgagcaac gaacaacata aagaacatat tataccgttg agtacgtatc ttggcgtttt
     2161 tagcgcactg atagctctca cggcgctgac ggtatggatc gcccaatttc acttcggcga
     2221 atggaacctg atcgtggcga tgtccgtcgc agtagtgaaa gcacttttgg tagcttttat
     2281 tttcatgcat cttttgtatg ataataaatt gtacgccacg gtgtttgccg gctcgattgt
     2341 atttttagcc gtatttattg tattcaccat gtatgataca ttgcgtcgtg atgatatcta
     2401 cgaacaagta ggtaaaccca tcaagtccaa agcgatcatc tatgataata tgcccgtcaa
     2461 gaaagattct acggcgcata gcggacatta ataggatccg tgaaagcgat ttgatttgaa
     2521 ccgatttcgg caatttgcct tattttcaac catcgccacg tacgtactta ttttcgttgg
     2581 cggtttggta cgagtttcag gggccggtct gggatgtccg gactggcctc aatgttttgg
     2641 tcattggttt ccaccgacca gtgtagatca gatccctccc catattgacc ccagtctttt
     2701 taatatgaca ttggcctgga tcgaatacgg caatcgtatt gtcggtgtta cgataggctt
     2761 tcttattgcc ggtacggctt ggcttgctat aaaacattac cggcatgaca aacgtatttt
     2821 gacagcgtca gttgcagcag cgattttggt ggctttccaa ggatggcaag ggagtcgtgt
     2881 cgtcaagtcc caactcaacc cgcatatcat ctccatccat tttgctttgg cgcttttgat
     2941 catcagtctg atggtttacg tcactttccg cgcatataaa cctgaacaag aaataaagaa
     3001 catcagcgat gccgtatatc gttctgtttt cggttggata aagattatgt gggttgtcaa
     3061 tctcctgcaa attttacttg gaacggaagt acggggtgca ttggaagtat tggctaatgc
     3121 atttccgttg atgagcgaag ccgaactaat gcgccaatcg aattatgtca gctatattca
     3181 cggtgtcgtc ggtgtgatca tcgtaggagc gacctggctt tttcacgaac aaatacagcg
     3241 tgtgcgcaca caaatcccgg cctatatcgt tacgatttca aatatcggcc ttttacttat
     3301 gatcatccaa ttgttcatcg gtgtgctcct ggtaacgatg gatcgtccgc cgcttttgca
     3361 aatcgtgcat ctttggatat ccagttttca gatcgggttg ttggtgatct tttatgccgc
     3421 atggcgccgc gataatcaat cttctgagag gaaaacggta tgaatacgaa tctgattatg
     3481 cgtttaagta tcggtttttt tgtgctcatg cttatttcgg cgatcgttat ttataaagcc
     3541 aaagaatcgc agagcgattt gccggtgtat gccgaagtaa cgccgtttgc catgaccgaa
     3601 cgaagcggtg aggcttttac aaacgaaaag ttcaaggggc acatcaccgt tgtcaatttt
     3661 ttcttcgcca cgtgtgcggg gccgtgcccg gcgatgaatg cccgtgtagc cgagctttat
     3721 cgcgcttttg cagggaataa ccccgtacgg ttcgtgtcgg tcacgataga tccggatcgg
     3781 gattcgctgg tcgcgcttcg taattatgcg atgtcgttca acgtaaacga taatcgctgg
     3841 tcttttttgc gcggcgatct gaaaagtgtt gagcacctgt cggaaaatat ttttatggtg
     3901 ggaagcggag caccgacact ccatagtacg aaactgattt tagtggatca cttgggccga
     3961 ataagaggtt actatagcag cgaagaacct gcaagtctga caatactcaa aacacatatc
     4021 cgtgaacttg taagagagat gccatcatga acgccgctga tttgccattt atcaatgcca
     4081 tactcaattc cgtgagcacc gtgtttctta cgttgggata catagctatc cgaaaaaaag
     4141 atcgtgttca gcataaacgc tatatggtca cggcgcttat ttcgtcagcg ctttttctca
     4201 cgtgctatct gatttatcat tatcaggtcg gttcggttcc ttatccgtat tacgattgga
     4261 caaggccggt atatttcgtc attttagtgc cacacattat actggccgca attatgtcgc
     4321 cttttattat cagcgccgtt cattatgcat ggattgggga gtttgcgaaa cacaaaagac
     4381 taatgcggtg ggtctggccg gtgtggatgt tcgtatcggt gagcggagtt gttatctatc
     4441 tgatgttata tcgcttctaa attaatcttc attttgccga caatgcgggc aaattcgctt
     4501 tcgacgaaag gtttgtacga cgagaactta gcagaaaacg gatgtttgat tttatttttt
     4561 gtagcatcca tcaaatcggc tatcaattct ttttgtgcat tgatgaggtc gtcgtaataa
     4621 ataggctcgc ccgcatcctt accttcgttc ttcatttttt cagcttgcgt atcccttaaa
     4681 aaatcaaatc ccttttctgc cgcaagcatg gcaataaggg ggtgagcttc cggcggggcc
     4741 gtattaagag ggtgaaacaa ggtatctttg taacgatcca taatttcatc cacggaatct
     4801 ctgtttcggc tgcatttttt aagcaggata ttttcaaaac taagggcata gcatgcagaa
     4861 gcttgaggta tatttcctgt ttgaaaatag agtgtgccca agtggcttag ggaaacagcc
     4921 agcttttcga tttcaacatt atacagcgtt tcatcgccca ggtccgggac gcgcgctgtt
     4981 tttacgacgt caaacacgcg agcaaaattg tctttttcgc gttcaataaa actaaaattg
     5041 acttcgccgc gcgaataacg ttcggctttg agcaaagcat cgcaccaata atggccgatc
     5101 gtaatggccg atatataatc tacaaattcc ttgttttcag tgactgtttg cagaacgcca
     5161 tccatgcggg acaaacattc ttcgtagcgc tggccgcgaa gcagaaaaaa taccaactgg
     5221 ggcatgacat aagcaaccaa ctcaggattg gaaagtttgg aagccaaatc ttcaatgatt
     5281 tcgatttcct catccaacac tttttcctga agacgattga cgacacgttc tgtcgaaacg
     5341 cgcagtgcct cctgaagttg tagatgcagt tcgttgcgca ggcgtggata atccggtagt
     5401 aaccggaaca gctcaatagc gcgctcgcac aataaactgc cctgtgcaaa atcagccgcc
     5461 agcgaggtgt gcatcgcagt ctggcacaag gaataaatca aaatactcat cgcccgatcc
     5521 gaacgcgcgt gatgcgattt caagtcggat tcaaattttt gaatggtttt gcggatgtct
     5581 gaagtggaac gatgatgaag ccgacggctc aaaaggccca ctaaaagtaa caccaggata
     5641 cgctgatctt cgatcaaaga aatgacattt tgtcttaaaa attgggcata gtttccgcga
     5701 actgtttcat cagtttcttt gcgcaccggt ttttcattcg ttttaggccg catctggatt
     5761 atcttattcc agtactcgcg caatgggcct tgcttgtgtg tgccggtgac ggaggtagga
     5821 ttatatacgg tttctaaggt acggcgattt ttttcaaacg tacgcaaaag cggagcggaa
     5881 acacgaacct catggttttg atgaaacaaa tgcaaaagat cggcataagt ttcgcgtatg
     5941 cagtgccatg atgatttatt ggattctacc gccagttgcg cgagaatatt tacaagactc
     6001 cgaatttcgc cgttagagcg gcgctcttcc tgaagcggtg cagtagacat agactattta
     6061 gatacgttta tttaggtgtg agatacccga ttactaatca actaatttat cataaaatcg
     6121 ggcgttcgca aggcaaaaat cataacaggg tatttaattt atcagctaaa ataaaatcca
     6181 attccgtaat tccgccgaca tcatgcgtat agagtgtaat atgaacgcgg ttatatacgt
     6241 tggaccagtc cggatggtga tttaattttt cagcgataag agccgctgcc gtcataaaac
     6301 gaaatgcttc gacaaaatcg gagaaaataa attctctata caacttgccg tcgcggagct
     6361 tccaatccgg aagtgttttc aggcgatttt caatttcata ggtttcaagt ttttttcgac
     6421 tcatggttac agctttttga atcggcgagt tacttcatcc caatgaatga ggttccacca
     6481 tgcttgtacg tagtcggcgc gtttgttttg gtacttcaga taataagcgt gctcccatac
     6541 gtccaaagcc aagagcggcg tgcctttaaa atcggaaaca tccatcaatg gattatcctg
     6601 attaggcgtt gagccgattt cgagttttcc gtcgcgtaca acgagccatg cccacccgga
     6661 gccaaatcgg ctcatcgctg tggcgttaaa tttagtttta aattcatcaa agcttccgaa
     6721 ggcggtatta atcgcagctg ccagttcgcc gctcggtgca ccgccgctcc ccggtttcat
     6781 gatttgccaa aacatggagt gattccaatg cccgccaccg ttgtttcgta tggctgtcga
     6841 atattttgaa atatgacgca tcaaagtatc taaatccggt ttatctattt tagcctgaat
     6901 gacggcatcg ttgagtttat ctacataggc tttatgatgg cgggaatgat gga
//
LOCUS       DAOQNQ010000175         6944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_4149_length_6944_cov_5.809697, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000175 DAOQNQ010000000
VERSION     DAOQNQ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6944
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4149_length_6944_cov_5.809697"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1615
                     /locus_tag="PK754_10725"
     CDS             125..1615
                     /locus_tag="PK754_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNB10023.1"
                     /translation="MAQKFQNLINGVWKDSVSGETFENRNPAKWNEDLIGIFPSSNAD
                     DVNEAVAAAKEAFKKWRLVPAPKRGDVLKIVGDMMLARKDEIAFEMTREMGKVFAETK
                     GDVQEGIDTAYYAATEGRRLFGHTVPSELANKFAMSVRMPIGVAGLICPFNFPLAIPT
                     WKMFPALLCGNTVVFKPAEDTPKTGSMLLEVLIEAGIPAGVVNLVHGFGEDVGATIVS
                     HPKIDLISFTGSSQTGSMIASQCGKDLKHVSLEMGGKNAQVVMDDANLELALDGVLWG
                     AFGTTGQRCTATSRLILHEAIHDKFVDMLIKAAQKLVIGDGLKPGNQVGPLIHEESLK
                     KVQRYVEIGKSEGAKLALGGERHTDGDCAKGWFFKPTIFTDVKAGMRIEQEEIFGPVL
                     SVIKVKNIDEAITVLNGTKYGLSSSVYTKDVNNSFKAMRDIKTGITYINAPTIGAEAH
                     LPFGGVKATGNGHREGGWPVFDFYSETKTVYVDYSDKLQRAQIDNY"
     gene            1793..3145
                     /gene="lat"
                     /locus_tag="PK754_10730"
     CDS             1793..3145
                     /gene="lat"
                     /locus_tag="PK754_10730"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HNB10024.1"
                     /translation="MEKIQIKPQEVHETLKKHILADGYDFVLDTKKSHGSWVVDSKTG
                     KEYLDFYSFFGSSPIGMNHPAMMTSEFREGLIDAATNNPANADVYTVAMAEFVATFEK
                     TGIPEYLPHMFMISGGALAIENALKTAFDWKVRKQHPNGFSSIEQEEDYVNSMKVIYF
                     KEAFHGRSGYTMTMTNTHYRYKVQYFPKFNWIKAPNPKMVFPLNDTNLADIKKREEES
                     LTFIREQVKRYKNQIAALILEPIQAEGGDNHFRVEFHKELRKICDENEIIMIYDEVQT
                     GVGLTGSFWAHEKIGVKPDILAFGKKMQICGILAGKRIDEVKDNVFQKSGRINSTWGG
                     NLADMYRATWYLRIIESEKLVENADKVGAHLLGKLQDLTKDFTQVTNVRGAGLMIAFD
                     LPTEQLRDAVHKKTPEFGLLVLKCGEKTIRLRPALNLTMAEADMGVERIAKALKEILK
                     "
     gene            3218..3292
                     /locus_tag="PK754_10735"
     tRNA            3218..3292
                     /locus_tag="PK754_10735"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3253..3255,aa:Pro,seq:tgg)
     gene            3343..3603
                     /locus_tag="PK754_10740"
     CDS             3343..3603
                     /locus_tag="PK754_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNB10025.1"
                     /translation="MKFSVYILYSKSCRRYYVGQTQDLERRLEEHNHGLSPSTRNGAP
                     WVIVYQFACDTRSDAMRIEKQIKKQGAARYIERQALGVTPKA"
     assembly_gap    3736..3835
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4046..>6944
                     /locus_tag="PK754_10745"
     CDS             4046..>6944
                     /locus_tag="PK754_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10026.1"
                     /translation="MKSILILTVLVILRGATLFSDVVAQEESSSAPKISTFATGKDIL
                     GVASNSVNLFSGDVHLPLALLSVPGRNGLDVGVNISYNSNIQNQNSTWNLEAPTGIMG
                     LGWSFGYEKIIVNHKSTGSIRDDDYYHISGSAGNQLIRTGTAPDGRLIYELKNYQFWK
                     IRRDTTYDWWEITKEDGTVFRFGGGVYSYNNRKYSEGNSVQWSVKWGNWVGSGTQDSL
                     QRQFATAWNLSKIKNISGDSVRFEYLNVDTTVKAGKPHTEASYLRRIIDVYGRRVEFI
                     LSDKLYNPSGQKEYQDPTTRFAEPDPYQDYYERKFLDSIEVFNDLNEKLMCIDLDYVI
                     LGSDSLAKRYLTSIIQRNAVYQSLPGIKFEYHTTGSLSGALKRITTPLGAKVEYTYTT
                     VNIKNSQRDLTVVRPQNCHDARVWTGDDYILVTWINNSDDSIKVAAYVWEGRWVYSGI
                     LATIGNVDYSLSQGYDFEAVIEKDFFGILVNYNRSSSTKYLHLFHKKPNAPDKWDYYT
                     QSLNVVNIQDNDVTFFSGERYVVVFGEESGKLFRFTWNGASWSSIEGPQLDSGSEDDF
                     FGTGAPDYFIFHDQNEGALDSIKFYYLDELGNWNIKLLNPTYSFNTDGGGDNPTYWYT
                     GPNFCVAMIKGQDDRIYWWSEEFDITQPHDTIVGLDYDSKVYINDAQISIHNMTSDHK
                     DKVRLYRFDGQKWVDKSIDNTTTHLYSATSSFGPDLAIRGSIQSEPAKIAEYNPSSGQ
                     WEERGLPNGGPRVGTRYYLFPNHDSIFYKMPNGQWRFAGKVSGDYTAGPRHGFNFTTC
                     THDSFPKKTGRAYILKNGQLKTTYISPYHTYNQYTSGILQNSDMTGAFACVFSTSSFD
                     TLVLHRVVGDVMTGRQQDFPVSSVTVYSGVDTTTVTYTYNDCTATYDPSGNFAEYHEV
                     KSTPSGGISGYTTTYFINSLPDTVAGFNVSYLDQEMKDNIGYLKGMQYYEQI"
BASE COUNT         2078 a         1338 c         1591 g         1837 t
ORIGIN      
        1 gcagtttctt gaagtaactt aattattttc ataccgattt ttatccattt cttttacatt
       61 taatcccatt gatagtttaa ttattttgta gaaactatat taataaggag aatttactac
      121 tattatggca caaaaatttc agaatttaat aaacggagtc tggaaagatt cagtatcagg
      181 cgaaacattt gaaaacagaa atcccgcaaa atggaatgaa gatttaatcg gaatatttcc
      241 ttcttcaaat gcagatgatg taaacgaagc agttgctgca gctaaagaag catttaaaaa
      301 atggaggctt gtacctgcgc ctaaaagagg agacgtgtta aaaattgtag gcgatatgat
      361 gcttgcgcgt aaagatgaaa ttgcatttga aatgacccgc gaaatgggaa aagtatttgc
      421 cgaaacaaaa ggcgatgtac aagaaggtat tgatacagcc tattacgcag cgactgaagg
      481 gcgccgcctt ttcggtcata ccgttccttc ggaattggcc aataaatttg ccatgtcggt
      541 tcgtatgccg atcggtgttg cggggttgat ttgtccgttt aattttccgt tagccatacc
      601 gacgtggaag atgttcccgg cgctgctttg cgggaatact gtcgttttca aaccggctga
      661 agacacgccg aaaaccggaa gtatgttgct cgaagttttg atcgaagcgg gaattccggc
      721 aggcgtagtt aaccttgtgc acggtttcgg tgaagatgtc ggtgcaacga tcgtaagtca
      781 tccgaaaatt gatctgatca gctttaccgg ttcgtcccaa accggcagta tgatcgcatc
      841 acaatgcggt aaagacctta agcatgtttc ccttgagatg ggcggaaaaa atgcacaagt
      901 cgttatggac gatgccaatc tcgaattggc tcttgacggc gttttatggg gcgcattcgg
      961 tacgacagga cagcgctgta ccgcaacgag ccgattgatt ttgcatgaag cgattcatga
     1021 taaatttgta gatatgctga tcaaggcggc tcagaaactt gtcatcggtg acggtcttaa
     1081 acccggaaat caagttggtc cgttgattca cgaagagtca ctcaaaaaag ttcagcgtta
     1141 tgtcgagatc ggtaagagcg agggtgcaaa actggcgctt ggtggtgaac gtcataccga
     1201 cggcgattgt gccaaaggat ggttctttaa acccacgatt tttaccgacg tcaaagcagg
     1261 catgcgtatc gaacaagaag aaattttcgg tcccgtgctc tcagtgatca aagtaaaaaa
     1321 tattgatgaa gcgatcacgg tgcttaacgg taccaaatac ggcttatctt catcggtcta
     1381 taccaaagat gtcaataaca gcttcaaagc catgcgcgac atcaaaaccg gtatcacgta
     1441 tatcaatgca ccgacgatcg gtgccgaagc acatttgcct tttggcggtg tcaaagctac
     1501 gggtaacgga caccgtgaag gcggttggcc ggtatttgat ttttacagtg aaaccaaaac
     1561 agtatacgtg gactatagcg ataaactgca acgtgcacag attgataatt actaaaaaac
     1621 catgagtgaa tttttccaaa atacatccaa cggcacagcg caacgtcggg gatggcttct
     1681 gcaacaagaa gctgtcggcg aagtgcgtca ttgcttgctc aaaccggaat ggcgcgtgtg
     1741 agatcggttc ttttttgtag ataattttta attattaaaa aaggataccg acatggaaaa
     1801 aattcaaatc aaaccgcaag aagttcacga aacactcaaa aaacacattc tcgccgatgg
     1861 ttacgatttt gtactcgata caaaaaagag ccacggttca tgggtagtag attctaaaac
     1921 cggtaaagaa tatttggatt tttattcgtt ttttggatcg tcaccgatcg gtatgaatca
     1981 cccggccatg atgacatctg aatttcgtga aggtctcatt gatgccgcga ctaataatcc
     2041 tgcgaatgcc gatgtatata cggtagcgat ggctgaattc gttgcgacat tcgaaaaaac
     2101 cggtattccc gaatatcttc ctcacatgtt tatgatttcc ggcggcgctc tggccatcga
     2161 aaatgcgctt aaaacggcgt ttgactggaa agtccgtaag caacatccca acgggttcag
     2221 ttcgatcgag caagaagaag actacgtcaa ttccatgaaa gtgatttatt tcaaggaagc
     2281 atttcacggt cgtagtggtt atacgatgac gatgaccaat acgcattatc ggtataaagt
     2341 gcagtatttc ccgaaattca actggatcaa agcgccgaac cccaagatgg tatttccttt
     2401 aaatgatacc aatcttgccg acatcaaaaa acgggaagaa gaatcgctaa catttattcg
     2461 cgagcaagtg aagcgttata aaaatcaaat cgctgcgttg attttagaac cgattcaggc
     2521 cgaaggcggg gataatcatt ttcgtgttga atttcacaaa gagctgcgaa aaatctgtga
     2581 tgaaaacgaa atcatcatga tctacgatga agtgcagacc ggcgtcggtc taaccggtag
     2641 cttttgggcg catgagaaaa tcggcgttaa acccgatatt cttgcgtttg gtaaaaaaat
     2701 gcagatatgc ggtattcttg ccggcaaacg tattgatgag gtaaaggata acgtatttca
     2761 aaaatcggga cgaatcaatt cgacctgggg cggaaatttg gcggatatgt accgtgcgac
     2821 gtggtatctt cgtattatag aatcagaaaa attggtagaa aatgcggata aagtcggagc
     2881 tcatttgctt ggcaaactgc aagatctgac caaagatttt acacaagtta ccaatgtacg
     2941 cggcgcgggt ctgatgatcg catttgattt accgaccgaa caactccgtg atgccgtcca
     3001 taaaaaaacg ccggaattcg gtttgttggt tttgaaatgc ggtgaaaaaa caatccgcct
     3061 tcgaccggca ctaaacctta caatggcgga agccgacatg ggcgttgagc gaattgcgaa
     3121 agcattaaaa gaaatcttga aataataaat aatttaccgt atatcagacg cccgtgctaa
     3181 aagtgatctt aaacacgggt tgtttgatag ataggttcgg ggtgtagcgc agcccggtta
     3241 gcgcgcctgc tttgggagca ggatgtcagg agttcgaatc ccctcacccc gacagagaga
     3301 aatgagtaac agccacgtta aaacgtggct gttgtcgtta ggatgaaatt ttcagtatat
     3361 atattgtaca gtaaaagttg ccggcgatat tatgttgggc aaactcaaga tttagagcgt
     3421 aggcttgagg agcataatca tggattatct ccttccacaa gaaatggcgc gccgtgggta
     3481 atcgtttatc agtttgcatg cgatacaaga tcagatgcga tgcgaatcga gaaacagatt
     3541 aaaaaacaag gcgcagcgcg atatattgaa cggcaagctt tgggagtcac gccaaaggcg
     3601 tgatcaggag ttcgaatccc ctcatcccga cagagagaaa tgagtaacag ccacgttaaa
     3661 acgtggctgt tttcgttaga atgaaatttt cagtatatat attgtacagt aaaagttgcc
     3721 ggcgatatta tgttgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttgca
     3841 tgcgatacaa gatcagatgc gatgcgaatc gagaaacaga ttaaaaaaca tagccgtatt
     3901 ttcgaacacg gttacccgcc gatgttcaaa tgtacgaagt aaaaaaaagc ttgacatggg
     3961 gtttatttta gtttttctta tgtagtacgg tagccttacc atatatctcg acgcgtcata
     4021 actacgataa aaaaataggg ggagtatgaa atccatttta atcctaacag tattagtgat
     4081 tttgagaggt gctacgttat tttctgatgt cgtcgctcaa gaggaaagct cgtctgcacc
     4141 aaaaatttct acgtttgcta ccggaaagga tattcttggg gtagcttcaa atagcgtcaa
     4201 tctgttttcc ggggatgtgc atttacctct tgcactttta tcggttccag gcagaaatgg
     4261 cttggatgtc ggtgtcaata tttcgtacaa cagtaacatc caaaatcaaa attccacatg
     4321 gaatttggaa gcgccaaccg gcattatggg ccttggctgg agtttcggat acgaaaaaat
     4381 aatagtaaat cataaatcga ccggctccat ccgcgacgat gattactacc atatcagtgg
     4441 ttcagcaggc aatcaactca taagaacggg tacggcccca gacgggcggc ttatatatga
     4501 attgaaaaat tatcaatttt ggaagattcg tcgagatacg acctatgact ggtgggagat
     4561 aacaaaggaa gacggaaccg ttttcagatt cggagggggc gtatattctt ataataatcg
     4621 taaatattct gaaggcaatt ctgttcagtg gagtgtcaaa tggggtaatt gggtcggatc
     4681 aggtactcag gactccctgc aacgacaatt tgccacggca tggaatctat ctaaaattaa
     4741 aaacatctcc ggagattcgg ttcgattcga atatcttaat gtagacacaa ccgtaaaagc
     4801 gggtaagccg catacagaag catcttatct aagacgcatc attgatgtct acggacgtcg
     4861 tgttgaattt attctttctg acaaattata caacccatcc ggtcagaagg agtatcaaga
     4921 tccgacaacc cgttttgccg aacccgatcc ttatcaggat tattatgaaa gaaaattctt
     4981 agactccata gaggtattta atgacctaaa cgaaaaactg atgtgtattg atttggatta
     5041 tgttattctg ggaagcgaca gtcttgccaa acggtatctg acttctataa ttcagagaaa
     5101 tgcagtttat caatcattac cgggaattaa atttgagtat cataccaccg gtagtttatc
     5161 cggcgcatta aaacgaataa ccacgccgtt aggagctaag gttgagtaca cctatactac
     5221 cgttaatata aaaaactcgc aaagggacct gacggtagta cgtcctcaaa actgccatga
     5281 tgcgagggta tggaccggag atgactacat actcgtaaca tggatcaata atagcgatga
     5341 ttcgataaag gttgcggcct atgtgtggga agggcggtgg gtttatagcg gaattttggc
     5401 aacgatcggc aacgttgatt atagtttatc ccaaggatat gattttgaag cggtgattga
     5461 aaaggatttt tttggtattt tggttaacta caatcgctca agttctacaa aatatctcca
     5521 tttatttcac aagaaaccaa atgcgccgga caaatgggat tattataccc aatcgctcaa
     5581 cgtagtaaat attcaggata atgatgttac cttcttttcc ggagagcggt atgtggttgt
     5641 tttcggagaa gaatcaggca aattgttcag atttacttgg aatggcgctt catggtcttc
     5701 catcgaaggc ccgcaattgg attccggtag cgaggacgat ttttttggaa caggagcgcc
     5761 ggattacttc atttttcatg atcagaatga aggcgcactg gatagcatca agttctacta
     5821 cctggatgag ttgggaaact ggaacattaa actactaaac ccaacctata gtttcaatac
     5881 ggatggcgga ggggataatc caacgtattg gtataccgga cccaattttt gcgtagcaat
     5941 gatcaaaggt caagatgaca gaatctactg gtggtcggaa gagtttgata tcactcaacc
     6001 tcatgatacc atagtgggtc ttgactatga ttctaaagtg tatatcaatg atgcgcaaat
     6061 aagcattcac aatatgacgt ccgatcataa agataaagta agactttatc ggtttgacgg
     6121 tcaaaaatgg gtggataaat caatagacaa tacgaccacc catctctata gcgcgacatc
     6181 ttcatttgga ccggatcttg ccatacgcgg ctccatacaa tcagaaccgg caaaaattgc
     6241 ggaatacaat ccgtcctcgg gccaatggga ggagcgaggg ttacccaatg gaggacctcg
     6301 agtgggtaca cgatattatt tatttcccaa tcatgatagt attttttaca aaatgcctaa
     6361 tgggcaatgg cgttttgcag gtaaggtgtc gggtgattat acagcgggac cgaggcatgg
     6421 ctttaatttt acaacgtgca cacacgactc ttttccaaaa aaaactggcc gagcgtatat
     6481 ccttaaaaac ggtcaattaa aaaccactta catatcgcct taccatacct ataatcaata
     6541 tacaagcgga atcctgcaaa acagtgacat gaccggagct tttgcgtgcg tctttagcac
     6601 gtcgtctttc gatacattgg tattgcatcg cgtggtgggt gatgttatga cagggcgtca
     6661 gcaagatttt cctgtgtcat cagtaacggt ctatagcggt gtggatacga ctacggtgac
     6721 ttatacttac aatgattgta cggcgacgta tgatccttca ggtaactttg ccgagtatca
     6781 tgaagtcaaa tccacacctt ccggcggtat ctccggatac acgacgacgt atttcatcaa
     6841 tagccttccg gatacagtag ccggttttaa tgtatcctat ttagatcaag agatgaaaga
     6901 caatatcggt tatctgaaag gcatgcaata ttacgagcaa attt
//
LOCUS       DAOQNQ010000176         6937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_4160_length_6937_cov_5.740918, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000176 DAOQNQ010000000
VERSION     DAOQNQ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6937
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4160_length_6937_cov_5.740918"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1648
                     /locus_tag="PK754_10750"
     CDS             <1..1648
                     /locus_tag="PK754_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNB10027.1"
                     /translation="YGIYKSRGVATMDSYVLSNRDMRWWTVAISIMATQASAITFIST
                     PGQGYADGMRFVQFYFGLPLAMVLLCITAVPFYRRLQVYTAYEFLEKQFDLKTRALAS
                     FLFLIQRGLGAGLTIYAPSLILSLLLGWNLVLTNLLIGGLVIIYTTTGGARAVNWTQF
                     HQMMIILTGMAAAFVTVIALLPDGISVNDALHLAGSQGKMNTVDFTWDWDNRYTLWSG
                     LIGGFFLQLSYFGTDQSQVGRYLTGQSIAQTRLGLLTNGLLKIPMQFLILLLGVMVFA
                     FYQFTMPPVFFNRLETERIYQSSYAADYVRLENDHTGLFKRQQEILHVWNAAKESGDE
                     MRMEEQETSLREINKQMAAVKEKAKDLIKAHDPSANTNDTNYIFLTFVLDYLPVGLVG
                     LIIAAIFAASMSSTSAELNALASTTVVDIYRRMFRSDASDAHYVRVSKWATVFWGAFA
                     VGFAELAGQLGSLIEAVNILGSLFYGTILGIFLLAFYWKKVGGTSAFTAAIIAEVIVF
                     LCYFFTPISFLWYNVIGCALVVGLAWVIYFFRQKTTLFQG"
     gene            complement(1718..2758)
                     /locus_tag="PK754_10755"
     CDS             complement(1718..2758)
                     /locus_tag="PK754_10755"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNB10028.1"
                     /translation="MKKNFFYVFIAIAGIITAHAQQRLKTPDASPRAVVEQTVGMTEI
                     RVTYYRPAVHERIIWGDLVPYGEVWRAGANENTTVSFSTPVIIGDREIPPGTYGLHMI
                     PMRDKWTIILNRDHKAWGSFFYNENHDVIRFNVEPENSAFQERLLYTFDGIESDRVTL
                     ALKWEKIRIPIPIRVDLHATVLAGFRDELTNVSGFFPEPYVQAVNYCVRNKINYDEAL
                     RWIDHSIELQESFANLNAKSRLLAAMGKNKEADSLNARAMYLATEVDLNNLGYSLMGE
                     NNFKEALAIFQKNAKEHPTSWNVFDSLGELYERMNDKKAAIENYRKALSLVKDGRNKQ
                     RLSETIKKLSGR"
     gene            complement(2773..3597)
                     /locus_tag="PK754_10760"
     CDS             complement(2773..3597)
                     /locus_tag="PK754_10760"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNB10029.1"
                     /translation="MKTWILFLAAAFSLNAQQISTPRPSPRATVSQTVGVTEMTVSYH
                     RPGVKGRTIWGELVPYGQVWRAGANESTTISFSDDVTIEGQKIPAGLYGIQMIPEANE
                     WIVILTKDAKLWGSFNYKPENDAVRIKVKPTSAEMIERLRYSFEDVNDNSTVLTMQWE
                     KLKIAFKIETDTPNLTFTKARAAMNWSGLMQAAAYCLDNNTNLDEGMRWIDLSIGMNE
                     NYYNLRTKAQFVDKTGKRADAIKVMERAMELSKKMPSPPFDIKRMEEVLNEWKKKK"
     gene            3722..4417
                     /locus_tag="PK754_10765"
     CDS             3722..4417
                     /locus_tag="PK754_10765"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNB10030.1"
                     /translation="MQNKRSALIVGASGLIGQHLLNALLEDRDYDRVIVLVRKPIAFT
                     HAKLSQHVVEFDKLTNAAHWIKAHDVFCTLGTTMRTAGSKQAFYKVDFTYPFECARIA
                     KENGVNNFLVVTAIGAKPSSGVFYSRVKGELENALETLRFRSLSIFRPSFLVGERSER
                     RIGEALGIVVFRTLGFLLVGRWKKYRAIRAQAVAEAMIEMAKHERAGKTIYESDVIQT
                     IFDVLIERRRRAV"
     gene            4439..5827
                     /locus_tag="PK754_10770"
     CDS             4439..5827
                     /locus_tag="PK754_10770"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB10031.1"
                     /translation="MDIQFCGAARSVTGSQHLIRINGQKLLLDCGLFQGRRQEAFERN
                     RTFPFDPASVDAMILSHAHIDHSGNIPNLVKQGFKGTIYCSEPTVDLCSVMLQDSAFI
                     QMRDAEHVNYKREKKDEPPIEPIYDIEDAQQAMRHFLGIGYGQAFEPLPGVRATLYNA
                     GHMFGSAMILLEITHNGRKVRFGFTGDLGRKNLPIIEDPHQLRALDYLICESTYGNKV
                     HDPVKEIHGEFVQVIQDAVARKGKIIIPAFSVERTQEIVYHLNLLYENKIVPEIPVFV
                     DSPLAVNVTEVFRKYPDYYDKDARNLLQSGDNPFSFEGLRYIREVKDSMKLNDINDPC
                     IIISASGMCEAGRILHHLKNNIENSNNTVLIVGYMAEHTLGRKLVDKAETVKIFGDTF
                     SVKARVHKINSFSGHADRTDLLNFTELVGKQASVFLVHGEEAQSVPFAESLKSNGFRH
                     VEIPERLQKITL"
     gene            5861..6658
                     /locus_tag="PK754_10775"
     CDS             5861..6658
                     /locus_tag="PK754_10775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HNB10032.1"
                     /translation="MKQHNFFIMGVVFFGLACSPKVDNNKKTTVSRNEYTMAALNWMQ
                     ISGEYRALCYQAFNVARMTLDVELSKKPAKKPAVVVDIDETVLDNSPYQARQIANGEQ
                     YPVGWQSWTDEAKAMAVPGAKEFLNYAVSKGADVFYITNRRPSEAPGTLNNLRSLGFP
                     QADTLHIMFREAESNKEARRRKVSETHSILVLCGDNLNDLSDAYDGRLNPERNQRVDQ
                     MKNEWGHRFIVLPNPLYGDWDGAMFNYNFNLSDAAKDSARKAVLRMN"
BASE COUNT         1756 a         1465 c         1685 g         2031 t
ORIGIN      
        1 gtacggtatc tataaaagcc gcggtgtggc cacaatggat tcgtatgttc tttccaatcg
       61 cgacatgcgg tggtggacgg ttgccatttc gatcatggcg acgcaggcga gtgcgatcac
      121 ttttatttcg acgccgggac agggttatgc cgacggtatg cgtttcgtac aattttattt
      181 tggtcttccg cttgcgatgg tgttgctttg tatcacggcc gtaccttttt atcggcgctt
      241 acaagtgtat acggcatacg aatttctgga aaaacaattt gacctcaaaa cgagggcgct
      301 ggcttcattt ctatttttga ttcagcgcgg acttggcgcc ggtcttacga tatatgcgcc
      361 gtcgctcatt ttgtcattac ttttgggttg gaatctggta ttgaccaatc tgctgatcgg
      421 cggactggtg atcatttata cgacgacagg cggtgcacgt gcggtgaact ggacacaatt
      481 tcatcaaatg atgattattt taaccggcat ggcggcggct tttgtgacgg taatagcgct
      541 tttgccggat ggcatttcgg tcaacgatgc attgcacctt gccggaagtc agggtaaaat
      601 gaatacggtt gattttacct gggattggga caatcgttat acgttgtggt cagggctcat
      661 cggcggtttt tttctacagt taagttattt cggaacagat caatcacagg tcggtcgtta
      721 ccttaccggt cagtctatcg cacagacgcg tctcggactt ttgaccaatg gtcttttgaa
      781 aatacctatg cagtttttga ttcttttact cggtgttatg gtttttgcat tttaccaatt
      841 taccatgccg cctgtttttt tcaatcgttt ggaaaccgaa cgtatatatc aaagttcgta
      901 tgccgccgat tatgtccgat tggaaaacga tcacacggga ttatttaaac ggcaacaaga
      961 gattctgcat gtgtggaatg ccgcaaaaga atccggtgat gaaatgcgta tggaagagca
     1021 agagacgagt ttgcgcgaaa tcaacaaaca gatggccgca gtgaaagaaa aggctaagga
     1081 cctgatcaaa gcccatgatc cttcggcgaa taccaacgat actaattata tttttctcac
     1141 gtttgtgctc gattatcttc cggtcggatt ggtagggctt atcatcgcgg ctatttttgc
     1201 ggcatccatg tcttccacgt cggcagaact caacgcattg gcctcgacta cagtggtgga
     1261 tatttaccgg cggatgtttc gctcagatgc ttcggatgcg cattacgtac gcgttagcaa
     1321 atgggctact gtattttggg gtgcattcgc tgttggattt gccgaattgg cgggtcagtt
     1381 aggttctctg attgaagccg tcaatattct gggatcctta ttttatggca cgatcctggg
     1441 aatattttta ttggcgtttt actggaaaaa agtgggtggc acatcggctt ttacggcggc
     1501 gatcatagcc gaagttattg tctttttatg ttactttttt acacccattt cttttttgtg
     1561 gtacaatgtg atcggatgtg ctttggtggt gggtttggcc tgggtgattt atttcttcag
     1621 acaaaaaaca acgttgttcc aaggatagta acctttttgt tttaccatgg cttttaataa
     1681 ctacccttga aacaactttg atatgattta cctgcgatca gcgaccgctt aactttttga
     1741 tagtttcaga taaccgctgc ttattccgcc cgtcttttac cagtgataac gccttacgat
     1801 agttttcgat cgcggctttt ttatcgttca tgcgttcgta taactccccc aggctgtcaa
     1861 aaacattcca tgatgtcgga tgctctttgg cgtttttctg aaaaatcgcc aaggcctctt
     1921 tgaagttatt ttcgcccatg aggctatagc ccagattgtt gagatcaacc tccgtggcga
     1981 gatacatggc ccgcgcattg agcgaatcgg cttccttgtt ttttcccatt gctgccagca
     2041 ggcgcgattt tgcattcaga ttggcaaatg attcctgcag ctcaatcgag tgatcaatcc
     2101 aacgaagcgc ctcatcgtaa tttattttat tacgaacgca atagttgacg gcctgaacat
     2161 aaggttcagg gaaaaaaccg gacacgttgg ttaattcgtc tctgaaaccg gccaggacgg
     2221 tggcatgcag atcaacgcgg atcggaatcg gtattcggat tttttcccat ttgagcgcga
     2281 gggttacacg gtccgattcg attccgtcga aagtataaag caggcgttcc tgaaatgccg
     2341 agttttccgg ttcgacatta aaccgaataa cgtcgtggtt ctcattgtaa aaaaaactgc
     2401 cccatgcctt gtgatcgcgg ttgagtatga tcgtccactt gtcgcgcatc gggatcatat
     2461 gaaggccgta agtgccggga ggtatttctc tatcgccgat gattacgggg gtcgaaaacg
     2521 aaacggtcgt attttcattg gctccggcgc gccagacttc gccataaggc acgagatcgc
     2581 cccagatgat tcgttcatgc actgccggcc ggtagtaggt tacacgtatt tcggtcatac
     2641 cgacggtttg ttcgactacg gcacgcggac tggcatcggg cgttttgagg cgctgctggg
     2701 cgtgtgccgt gatgatcccc gcgatagcga tgaacacgta aaaaaaattt tttttcatga
     2761 aaactccgga aattattttt tctttttcca ctcgttgagc acttcttcca tgcgcttgat
     2821 atcaaagggc ggagagggca tttttttaga aagctccatg gcacgttcca tgactttgat
     2881 agcgtcggca cgttttccgg ttttatctac aaactgggct ttggtgcgga gattataata
     2941 attttcattc ataccgatgg acagatcaat ccacctcatg ccttcgtcga ggttggtatt
     3001 attatcaagg caatatgcag cggcctgcat aagtccgctc caattcatgg ccgcccgtgc
     3061 tttggtaaag gtcagattcg gtgtgtcagt ttctatttta aatgcgattt tcaatttttc
     3121 ccattgcata gtcagcacgg tgctattgtc gttgacatct tcaaatgaat atcgaagacg
     3181 ttcgatcatt tctgcggaag tgggtttgac tttgatgcgc acggcatcgt tttcgggttt
     3241 atagttaaat gacccccaaa gtttggcgtc tttggtcaaa ataacgatcc actcattggc
     3301 ctcaggtatc atctgaatgc catacaaacc ggcgggtatt ttctgacctt cgatggtgac
     3361 atcgtcagaa aaactgatgg tggtgctttc gttggccccg gcacgccaca cctgaccgta
     3421 aggcaccagt tcaccccaaa tcgtacgacc ctttacaccc ggacgatgat agcttacggt
     3481 catttccgta acaccgacgg tttgcgaaac ggttgcgcgg gggcttggcc tcggtgtcga
     3541 aatttgctgt gcatttaagg agaatgcggc cgctaaaaat aatatccagg ttttcatttt
     3601 accatcctct tcattttaga aattatgatt aatcaatgta aaagaaatgt tgtaattttt
     3661 ctactgtttt tagtcaaaaa ttagtttaaa ctaaaccacg gtttatcatc tttttagcac
     3721 tatgcaaaat aaacgttccg cattgatcgt aggcgcttcc ggtcttatcg gacagcatct
     3781 gctcaatgcg cttttagaag atcgggacta tgatcgcgtc attgttttgg tgcgtaagcc
     3841 tattgctttt acgcacgcaa aactgtcgca acacgtcgtg gaatttgata agttgaccaa
     3901 tgccgcgcat tggatcaaag cgcatgatgt gttttgtacg ctcggtacga cgatgcgcac
     3961 ggccggttca aaacaggctt tttacaaagt ggattttaca tatcccttcg aatgtgcacg
     4021 tatagcgaaa gaaaacggcg ttaataattt tttggtcgtt acggctatcg gtgcaaaacc
     4081 ttcttccggt gttttttaca gccgggttaa aggcgagctg gagaacgcgt tggaaacgtt
     4141 gaggtttcgt tcgttgtcaa ttttcaggcc ttcctttttg gtcggcgaaa gatccgagcg
     4201 acgcatcgga gaagcgttgg gaatcgttgt gtttcgaacg cttggctttt tattggtcgg
     4261 ccgatggaaa aaataccgcg caatacgtgc ccaggcggtc gcagaggcga tgatcgaaat
     4321 ggctaaacat gagcgtgccg gtaaaacgat atacgaatcg gatgttatac agactatttt
     4381 tgatgtattg attgaacggc gacgccgggc ggtataatta aattattacg gaagtatcat
     4441 ggatatacaa ttttgcgggg cagcccgcag tgtgaccggt tcgcaacatc tgatccgtat
     4501 caacggtcaa aaactgttat tggattgcgg cttattccag gggcggcgac aggaagcttt
     4561 tgaacggaat cgtacgttcc cgtttgatcc ggcttccgtt gatgcgatga ttctctcgca
     4621 tgcgcatatt gaccacagtg gcaatattcc gaacctcgtc aaacaaggtt ttaaaggtac
     4681 gatttattgt tcggaaccga cggtggattt atgttcggtg atgcttcagg attcggcttt
     4741 tatacaaatg cgcgatgcgg agcatgtcaa ctataagcgt gaaaaaaaag acgagcctcc
     4801 gattgagccg atatacgata tcgaagatgc gcagcaggcg atgcgtcatt ttttagggat
     4861 tggttatggt caggcgtttg agccgctgcc gggcgtgcgt gctacgctgt ataacgcagg
     4921 gcacatgttt ggttcggcga tgatcctgct ggagatcact cacaacgggc gcaaggttcg
     4981 atttggtttt accggtgact taggtcggaa aaatctgccg atcatcgaag atccgcatca
     5041 attgagagcg ttggattatc tcatttgtga aagtacctac ggcaacaaag ttcacgatcc
     5101 ggtgaaagaa atacacggcg aatttgtaca agtgatccag gatgccgttg cgcgtaaagg
     5161 caagattatc attccggcct ttagtgtgga gcgtacacaa gagatcgttt atcatctcaa
     5221 tttgctgtat gaaaacaaaa ttgtccctga gatacccgta tttgtggata gtccgcttgc
     5281 ggtgaatgtt acggaggtat ttcgcaaata ccctgattat tacgataaag atgcgcgtaa
     5341 tctcctgcaa agcggagata atcccttttc gtttgaagga ttgcgctata ttcgtgaagt
     5401 aaaagattcg atgaagctca acgacataaa cgatccatgc attatcattt cagcatccgg
     5461 tatgtgcgaa gccgggcgca ttttacatca tttgaaaaat aatattgaga attccaataa
     5521 caccgtgctc atcgtaggtt atatggcaga gcatacactg ggccgtaaat tggtagacaa
     5581 agccgagacg gtaaaaatat ttggcgatac gttttccgta aaagcgcgcg ttcataaaat
     5641 caattcgttc agcggtcatg cggaccgcac cgatttgctt aattttaccg aattggtcgg
     5701 caaacaagct tccgtttttt tggtacacgg cgaagaagcg caaagcgtgc catttgcgga
     5761 atcattaaaa agtaacggat ttcgccatgt tgagatcccg gaacggcttc agaaaataac
     5821 attataaatt atactataac cagctacagt aaggatgagc atgaaacagc ataatttttt
     5881 tatcatgggt gtggtttttt ttggattagc gtgtagtcca aaggttgaca ataataaaaa
     5941 aacaacggtc tcgcgaaatg agtacacgat ggccgcatta aactggatgc agatatccgg
     6001 tgaataccgt gccttgtgtt atcaggcttt taatgttgca cgtatgacac ttgatgtcga
     6061 gttatccaaa aagccggcta aaaaaccggc ggtcgtcgtg gatattgatg agacagtttt
     6121 agataacagc ccatatcaag cacgccaaat agccaacggc gaacaatatc cggtaggatg
     6181 gcagtcgtgg acggatgaag caaaggccat ggctgtcccc ggtgctaagg agtttttaaa
     6241 ttatgccgtt tccaagggtg cggatgtttt ttatatcacc aaccgtcgcc cgtctgaagc
     6301 gccgggtact ttgaataatt tgcgttcgct cggatttccg caagcggaca cgttgcatat
     6361 catgtttcgc gaagcggagt ccaataaaga agcgcgccgt cgtaaagtca gtgaaacaca
     6421 ttctattctt gtgttgtgcg gcgataacct gaatgattta tccgatgcct atgatggccg
     6481 tctgaatccc gaacgcaacc agcgtgttga tcaaatgaag aatgaatggg ggcaccgttt
     6541 tatagttcta cctaatccgt tatacggtga ttgggacggc gctatgttta attataattt
     6601 taacctatcc gatgccgcaa aagacagcgc gcgcaaagct gtgctaagaa tgaattaatt
     6661 tagctgattt gtattaaagt aacggataag agagcgtgca ttgtatgaaa taacgtcagc
     6721 ttacttattg aataagctca tactctaaaa ttcttattga caatcagtat attttcaact
     6781 actattggcg cactttaatg ctgtctttag tacagcaata caaaacgttc tgatctaaaa
     6841 atcagaacta ccggataacc cgatcggcat cactcagcgg acgggttgaa aataatcccg
     6901 gttcacgatg ttaaaatatg gcacaaatta atatcac
//
LOCUS       DAOQNQ010000177         6896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_4204_length_6896_cov_6.399649, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000177 DAOQNQ010000000
VERSION     DAOQNQ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4204_length_6896_cov_6.399649"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /gene="carB"
                     /locus_tag="PK754_10780"
     CDS             complement(<1..545)
                     /gene="carB"
                     /locus_tag="PK754_10780"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889521.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HNB10033.1"
                     /translation="MPKRTDIKSILVIGSGPIVIGQSCEFDYSGTQSCRALKEEGYRV
                     ILINSNPATIMTDPETADRIYIEPITPAMVEKIIERERPDALLPTMGGQTALNTSVEL
                     FKSGVLEKYNVEMIGAKYDSIQTAEDRELFKEAMERIGLHVPKGRFVRTVQDASEAVS
                     EFDYPIILRPSFTLGGTGGAVA"
     gene            complement(717..2522)
                     /locus_tag="PK754_10785"
     CDS             complement(717..2522)
                     /locus_tag="PK754_10785"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNB10034.1"
                     /translation="MKNAGFFLWVWVFSISLCVKELKAGGDEYWFLKNQAHTTPENKL
                     YDTPDWRIALDGPIRGSAAFSGGHVYIGTAKGTLYAVREKDGTVEWNRNFGSAIHSTP
                     AVKGNHLYFTDNSQHLYCVSAGDGKEKWRYAFDKALPYDWKFDYWYSSPVFWGENIIV
                     GGDDGYLHSVRLRDGKAQWKYKAESVIRSTPVIRDGIIYFGDCMGYVYAVDAATGKSK
                     WRSETEGVRIDQSQYIFDRKAVISSAALSEDVCVIGSRDGFLYGFNTIDGKQLWSVDY
                     KISWVNSTAAIREGIAVTATSDGRYVNAVEAKTGKEIWRFNTRQILWSSPLIINNHVY
                     AQSCDGVMHILNFKTGEKISEHRTITPIQSFSSPVFTGKTLLYTNDSGILYASFGRPT
                     PVESRTRKLALWMDKVGLQFGNNMDVRLKNYLNDIGYEAIDTNRFAEILTQSDSAPKT
                     IVVVISAAFPDTILQGAKNSLVRRFLDQGGAIINFGFNPTVFDPRIETYNFQKAEEIF
                     GVAYGPNDLRSFQGLYPSFATELGKKYQLPDFWTGFAGVNKNQVDEVLGLNENGLASS
                     WIKYYGANKKGAWIQIAMDRSNPVDYNFLKYFNAK"
     gene            complement(2607..3983)
                     /locus_tag="PK754_10790"
     CDS             complement(2607..3983)
                     /locus_tag="PK754_10790"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNB10035.1"
                     /translation="MIVMKFGGTSVQDAGAIRRVAEIVRRHAERKVLVVVSACAGVTD
                     QLVRLSQAIERRDEAAIGKIKETLIDKHKMEAQTLTLSAEEKEQLERFNGEFERCVNG
                     ASILHEVTPRTHDRLLSFGELWSSVLTLSAIRQTGQSISWLDARTVITTDNAYNAAHP
                     QTDALPLQVQQKIIPALERGIVITQGFLGATRDGHTTTLGRGGSDYSASLLGASLKAD
                     EIQIWTDVDGILTTDPRLVHVARQLTHVSFDEATELSQFGAKVLHPKTVLPAIQKNIP
                     IRVLNTFNPSNAGTCITREAIKGKGIVRSIAFKRGLEVMTVRFNDPEQKSPILAEIFA
                     RLQEAGTDVELVNTTERGIVLAAASNERWRVFCDHLPASWHITFQTGRALCALVGHLI
                     AEDFSNLTRALSVMQSEAIHVEAIAQGGSGHHWAVLLREHQLTPAVRLLHADFFENRA
                     SGQTVAVA"
     gene            complement(4011..4763)
                     /locus_tag="PK754_10795"
     CDS             complement(4011..4763)
                     /locus_tag="PK754_10795"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HNB10036.1"
                     /translation="MISLKDKVALITGGSRGIGAATAILLAEAGADIAINYLNSEANA
                     RRVVDRIQALGRKSAAIRADVSDDAQAKELVQSVILKFGRLDILVNNAGIWTRAPIGL
                     TDKSVWDATYLTNVQSYLSVTNAAVPHLMKSRGRIINITSTAGQRGEAFHSHYAASKG
                     AITAFTKSIAVELGPQGILVNNVAPGWVDNDLNREVFSDAGFKKTVIESIPIRRIASS
                     EDVAGAVLFLASDLSRHITGSTISVNGGAVLV"
     gene            complement(4760..5887)
                     /gene="metX"
                     /locus_tag="PK754_10800"
     CDS             complement(4760..5887)
                     /gene="metX"
                     /locus_tag="PK754_10800"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNB10037.1"
                     /translation="MNLLVSSMPSVFSTVKYTTVFDTIPLRLQQGGTLSNITVAYETY
                     GTLNNEKSNAVWVCHALTGNAHAYSDGTNVPGWWDGLIGKNLALDPDEYFIICSNILG
                     SCYGTTGPASHYPADGLPWRKRFPLITVRDMVQVQHALLEHLGIERLQAVIGGSLGGM
                     QVLEWALMYPEKVARIIPIATAASHSAWAIAWNEAARRAIVQDPAFQNGGYNEQPVKG
                     LGLARWIAMISYRSQKSYQDKFARGGQPCDQINPDFFYSDETAQPFAIEHYLDYQGKK
                     LTARFDALSYIRITQAMDRHDVTEGRGSLDYVLRSIRAKSLCIGIRSDVLYPAEEQKA
                     IARGIKNARYIELDSDDGHDAFLIKNELMNETIAKFLKENE"
     gene            complement(5884..>6896)
                     /locus_tag="PK754_10805"
     CDS             complement(5884..>6896)
                     /locus_tag="PK754_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNB10038.1"
                     /translation="SLLYGGTYNQFKVTLPRLGIDVKFVNGDRPEDFERLIDTKTKAL
                     YIETIGNPRLNIPDFQAIADVAHRHGIPLIVDNTFGAGGYIAQPIRFGADIVVHSATK
                     WIGGHGTAIGGVIVDAGTFDWGNGKFPLFTEPSPGYHGLKFWDIFGSAGPFGNIAFAI
                     RARVEGLRDIGPSQSPFNAFLLLQGLETLSLRVERHAQNALALAHWLKKQPQVSWVSY
                     PGFEDHPYHANAKKYLRPGHYGGVLVFGIKGGKEAGKKFINSLSLASLLANVGDAKTL
                     VIHPNSTTHQQLSDAEQHTSGVTPDLIRVSVGIEHIDDIIADFEQALHKTESLTVEES
                     AA"
BASE COUNT         1840 a         1764 c         1452 g         1840 t
ORIGIN      
        1 gctacagcgc cgcccgttcc gcccagcgta aaagacggtc ttaaaataat cgggtaatca
       61 aattcggata ccgcttcgct ggcatcctgc actgtacgaa caaatcgtcc tttgggtacg
      121 tgcaagccga tgcgttccat ggcttcttta aaaagttcgc gatcttcggc tgtttgaatg
      181 gaatcatact ttgcgccgat catttcgaca ttatattttt caagcacgcc ggatttgaaa
      241 agctccaccg acgtattgag tgccgtttgg ccgcccatag tcggtaacaa ggcatccggg
      301 cgctcgcgct caattatttt ttcaaccatc gccggcgtaa tcggttcgat ataaatacga
      361 tccgccgttt ccggatcggt catgatcgtg gcgggattac tgttgatcag aattacacgg
      421 tagccttctt ctttgagggc acggcaggat tgagttccgg aatagtcaaa ttcgcaagac
      481 tgacctatga cgatcggccc gctgccaatg actaaaattg atttgatgtc ggtacgtttg
      541 ggcacaaaat atccaagaaa ttttgatata tgttaaatcc tcgtaattta tcaaaattcc
      601 ttattaatgc aaggctggct ttcatttgta cagtccataa aaaaactccc aaaatatcgt
      661 atttttaaca tacgacatgt taggagttta aaagaactcg atgtagttgc ttagatctat
      721 ttagcgttaa aatactttaa aaaattataa tcaaccggat tgctgcgatc catggcgatt
      781 tgtatccacg ctcctttttt attggcaccg taatatttaa tccaggatga agctaacccg
      841 ttttcattaa gtcctaacac ttcatccacc tgatttttat tcactccggc aaaacccgtc
      901 caaaaatcag gcaactgata tttttttcca agttcggtag caaacgacgg atataaaccc
      961 tgaaaactgc ggagatcatt gggaccataa gccactccga aaatttcttc cgctttttgg
     1021 aaattatagg tctcaatgcg cggatcgaac acggtaggat taaatccaaa attgatgatc
     1081 gcaccgcctt gatcaagaaa tcggcgaacc aagctgtttt ttgcgccttg caatatcgta
     1141 tccggaaatg ccgcagaaat cactaccact attgtttttg gggccgaatc agattgtgtt
     1201 aagatttcag caaaacgatt ggtatcaatg gcctcgtagc caatatcgtt aagataattt
     1261 ttcaatctta catccatatt gttaccaaac tgcagtccta ctttatccat ccaaagcgca
     1321 agtttccgtg tacgcgattc gacaggggtc ggtctgccaa acgacgcata cagaataccg
     1381 ctgtcattgg tataaagcag cgttttgcct gtaaatacgg gtgacgaaaa cgattggatc
     1441 ggcgtgatcg tacgatgttc tgaaattttc tcaccggttt taaaatttaa aatatgcatg
     1501 acaccgtcac aactttgggc atacacatga ttgttgatga tcagaggtga cgaccataaa
     1561 atttgacgcg tattaaatcg ccagatttct ttaccggttt tggcttccac ggcatttaca
     1621 taacgtccgt cggatgttgc cgtcacggca ataccttcac gaatagccgc ggtcgaattg
     1681 acccaactta ttttataatc cacagaccac agttgtttac catctatcgt attaaaacca
     1741 tataaaaatc catcgcggct gccgatgaca caaacatcct cggacaacgc agccgaacta
     1801 atcaccgctt tgcgatcaaa aatgtactgt gattgatcta tgcgtacacc ttcggtttca
     1861 cttctccatt tcgacttccc ggtcgctgca tctactgcat agacataccc catacaatcg
     1921 ccaaaataaa taatcccgtc tctgataacc ggtgttgaac gaatcacgga ttccgcttta
     1981 tatttccatt gtgctttacc gtcacgtaac cgtacactat ggaggtagcc atcatcaccg
     2041 cccacgataa tattttcgcc ccaaaaaaca ggcgatgaat accaatagtc aaacttccaa
     2101 tcgtaaggca atgccttatc aaaagcataa cgccattttt ctttaccgtc acccgccgat
     2161 acacaataca aatgctgtga attgtcagta aaatacaaat gattgccttt taccgccggc
     2221 gtagaatgaa tagccgagcc aaaattacga ttccattcta ccgtaccgtc tttttcgcga
     2281 accgcataaa gcgttccttt ggctgtgccg atatatacgt gaccgcccga aaaagctgcc
     2341 gaaccgcgta tgggaccatc cagagcaata cgccaatcgg gtgtatcata cagtttattt
     2401 tcaggcgtgg tgtgggcctg atttttgaga aaccaatact catcaccacc ggctttcagt
     2461 tctttcacgc acaacgaaat actgaatacc caaacccaca aaaaaaaccc cgcatttttc
     2521 atgacaaacc tcatcattta ataacaacaa cggatgtgtt tagattaggt atgaggttac
     2581 gtatttattt aaatattgtt tcgtcgtcag gcaacggcta cggtttggcc ggatgctctg
     2641 ttttcaaaaa aatcggcatg caagagtcgc accgccggtg taagctgatg ttcgcggagc
     2701 agcaccgccc agtgatgccc tgatccccct tgtgcaatcg cttcgacatg aatggcttcg
     2761 gattgcatca cactcaatgc tcttgtcagg ttggaaaaat cttcggcgat caaatgacca
     2821 accaatgcac acaacgcccg tccggtctga aaagtaatat gccatgaggc cggcaggtga
     2881 tcacaaaata cccgccaacg ttcattggaa gcggccgcca gaacaatccc ccgctctgtc
     2941 gtgtttacca attcaacatc ggttcctgct tcctgcaacc gagcaaaaat ttctgcaaga
     3001 atcgggctct tttgttcagg atcattaaat cgtaccgtca taacttccaa gccccgctta
     3061 aacgcaatcg aacggacaat tccttttcct ttgatcgcct cccgcgtaat gcatgtaccg
     3121 gcattggatg gattgaatgt gttaagcaca cgaattggaa tgttcttttg aatcgccggt
     3181 aataccgttt taggatgcaa tactttagca ccaaattgcg acaactccgt agcttcatca
     3241 aagctgacat gcgtcagttg acgggcgaca tgcaccaggc gcggatccgt tgtgagaata
     3301 ccgtccacgt ccgtccatat ttgtatttca tccgctttga gcgacgcacc taaaagtgat
     3361 gcgctgtagt ccgaaccacc gcggcccagt gtggttgtgt gaccgtccct cgttgcaccg
     3421 agaaaacctt gggtgatcac gatgcccctt tctaaggcgg gtattatttt ttgctgaact
     3481 tgtaacggca gcgcatctgt ctgtggatgg gcggcgttgt aggcattatc cgttgttatc
     3541 acggttcgtg catccaacca cgaaatgctt tgacctgtct gacgaatcgc cgacaaagta
     3601 agaaccgatg accacaattc accgaacgac aaaagacgat catgcgtcct cggcgtcact
     3661 tcatgcaaaa tggatgcgcc gttgacacag cgttcaaatt caccgttaaa tcgttctaat
     3721 tgctcttttt cctccgccga caaagttagc gtttgagcct ccattttgtg tttatcaatt
     3781 aatgtttctt ttatcttgcc gatagcggct tcatcacgcc tttcaatagc ctgactcagc
     3841 cgtacaagtt gatcggtcac accggcacat gccgaaacca ctaccagcac cttgcgttca
     3901 gcgtggcgtc tgacgatttc ggccacccgg cgaatcgcac ccgcatcctg cacagaagta
     3961 ccgccaaatt tcataactat catgatatcc tcataaaatt ataattgaat ttaaactaat
     4021 acggcgcccc cattgacgct gatcgtcgaa ccggtgatat ggcggctcag atctgatgcc
     4081 aaaaataaaa cggcaccggc cacatcttcc gaggacgcaa tacgacgaat cgggatactc
     4141 tcaatcacgg tttttttgaa acccgcatca gaaaaaactt cgcggttaag atcattgtcc
     4201 acccaacccg gtgctacatt attcactaaa ataccctgcg gcccgagttc gacggctatg
     4261 gattttgtaa acgcagtgat cgctcctttg gatgcggcat aatgactgtg aaatgcttcc
     4321 ccgcgctggc cggctgtgga cgtgatatta ataatacgac cgcgtgattt catcaaatgc
     4381 ggaacggccg cattagtcac ggataaatag ctttgtacat tagtgaggta tgtagcatcc
     4441 cacacggatt tatcggtaag cccgatcgga gcgcgcgtcc agatacccgc attattaaca
     4501 agaatatcca atcgtccgaa tttcagaatc accgattgca ccaattcttt ggcttgcgcg
     4561 tcatcgctga catccgcacg gattgcggct gattttctac ctaatgcctg aatgcgatca
     4621 actacgcgcc gggcattcgc ctcactgttt aaataattta tggctatatc ggctccggcc
     4681 tcggccagta atatcgccgt ggcggctccg ataccacgcg aacctccggt gatcaaggca
     4741 actttatctt ttaacgatat cattcgtttt cctttaaaaa ttttgcaatc gtttcattca
     4801 tcaattcatt ttttatcaaa aaagcatcgt gaccgtcatc cgaatccaat tcgatatagc
     4861 gtgcattttt aattccccgc gcgatcgctt tttgttcctc cgcaggataa agcacgtccg
     4921 agcgaatgcc tatgcacaaa gatttagcac gtatgctacg cagtacatag tccaaggagc
     4981 cccggccttc agtcacatca tgacggtcca tggcttgcgt tattcggatg taactgagcg
     5041 catcaaaacg cgctgtaagt ttttttcctt ggtaatccaa gtaatgctca atcgcgaatg
     5101 gttgtgccgt ttcatccgaa taaaaaaaat cgggattaat ttgatcgcac ggctgaccac
     5161 cacgtgcaaa tttgtcctga taagattttt gcgagcgata cgaaatcata gcaatccacc
     5221 gtgccagacc gagtccctta accggctgtt cgttataccc gccattttga aatgcaggat
     5281 cttggactat ggcacgtcgt gctgcttcat tccaggcaat agcccaagct gaatggcttg
     5341 ctgccgtggc aataggtatg atccgcgcaa ctttttccgg atacatcagc gcccattcaa
     5401 gcacttgcat accacccagc gaaccgccga taacggcttg cagtcgctca atgcctaaat
     5461 gttccaaaag tgcgtgctgt acctgcacca tatcgcgtac tgtgatgagg ggaaatcgtt
     5521 tgcgccaggg taacccgtcc gccggataat gcgatgccgg gccggtcgta ccgtaacaac
     5581 tgccgagaat attactgcaa ataatgaagt attcgtccgg atcaagtgcc aaattttttc
     5641 cgatcaatcc gtcccaccaa ccgggtacgt ttgtaccgtc gctgtaggca tgcgcattac
     5701 ccgtcaatgc gtggcagacc caaacggcat ttgatttttc gttgttgagt gtaccatagg
     5761 tctcatacgc cacagtaatg ttcgataaag tacccccttg ctgaagacgc aaggggatcg
     5821 tatcaaacac cgtcgtatat tttactgtcg aaaaaaccga tggcatcgaa gatacaagca
     5881 gattcatgcg gcggattcct ccacagtcaa agattccgtc ttatgcaatg cttgttcaaa
     5941 atcagcgata atatcatcga tatgttcgat tcccactgaa acacgaatca aatccggcgt
     6001 cactccggag gtgtgttgct cggcatcgct taattgctga tgcgtggtcg aattggggtg
     6061 aatgaccaaa gttttagcat cgcctacatt ggccaaaaga ctagctaaag agagactgtt
     6121 gataaatttt ttcccggctt ctttcccgcc tttaattcca aataccagca ctccgccata
     6181 gtgacccgga cgtaaatatt ttttggcatt ggcgtgatac ggatgatctt caaaacccgg
     6241 atacgacacc cacgatactt gcggttgttt tttcaaccaa tgtgccagtg ctaacgcatt
     6301 ttgggcatgc cgttcgacgc gtaacgacag cgtttccaaa ccttgcagga gcagaaatgc
     6361 attaaaaggt gactgactgg ggccgatatc ccgcaaaccc tcaacacgcg cacgtatggc
     6421 aaaagcaata ttgccaaacg gccccgcact gccaaaaata tcccagaatt ttagtccatg
     6481 gtatcccgga ctgggttcgg taaacagagg aaatttaccg ttaccccaat caaacgtacc
     6541 ggcatcaacg atcacgccgc ctatcgccgt gccgtggcct ccgatccatt tggtcgccga
     6601 gtgcaccacg atgtcggcgc caaaacgaat gggttgtgcg atgtagccgc ctgcaccgaa
     6661 tgtattatca acaatcagcg gaataccatg acggtgcgcc acgtccgcga tcgcctgaaa
     6721 atccggaata ttcaatcgcg gattgccgat cgtttcgatg taaagcgctt tggttttcgt
     6781 atcaatgagt ctttcaaaat cttccggacg atccccgttg acaaacttca cgtcaatccc
     6841 gagacgcggt aaagtgactt taaattgatt gtatgtgccg ccatataata aactgg
//
LOCUS       DAOQNQ010000178         6890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_4207_length_6890_cov_5.829700, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000178 DAOQNQ010000000
VERSION     DAOQNQ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6890
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4207_length_6890_cov_5.829700"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PK754_10810"
     CDS             <1..472
                     /locus_tag="PK754_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNB10039.1"
                     /translation="YSGMMKAGLQIYLTGGGTKFRVPLHEDYKHMKKISEAGAAIEKV
                     KADEKLTFEKLTDVYYSGTKHEEHQPCHLVITPDDLANICNTRCKEEYGNPCQHFCPA
                     QVYNMITPEAGKPTQLRIDFSNCVHCKTCDIADPYQVINWVTPEGGGGPVYKNM"
     gene            532..1866
                     /locus_tag="PK754_10815"
     CDS             532..1866
                     /locus_tag="PK754_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10040.1"
                     /translation="MKLSWFILLITVCISNVNAQEAQSYFDDGGKMAVIDAATNTRLN
                     LFPSYEGFIEARLFETEDGEYMLEILYQANHKILRQRIPQNGDQVRALRERIASGKTQ
                     QQASVINQEGRVRLLSVAAGASLGYYAIAVPIIAKVEGRAAVGLYLLTGGLGFYVPYQ
                     ITKNMSITHGAADGYTYGLFHGAGYGAALNFLASGGDITGRQFLFSTSVGSIAGSILG
                     YQYAKRNNLSSGDVAVYNIGGLYGTGMGLGAAALTETKKSRIYAASGLVGNMAGLVIG
                     HSFLNAQHYTSGDMDMVMNSGALGAYLLPSVLLPTKLKDNRIYIAATMTGGTLGLMTG
                     NNLIRGKDFTASQSRVIALGGAAGLLTGAGIAYLVAPEDKPRWYIASSAVGGLIGFSW
                     MYASNKDKVEHESGNKTSLKIRFMPENYLMASKMKNKKDAPALPVMSAELRF"
     gene            1937..2890
                     /locus_tag="PK754_10820"
     CDS             1937..2890
                     /locus_tag="PK754_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10041.1"
                     /translation="MKNLIYVVVVCLVFVSEESVYTQTSKSDDKKTDMVNVCLKSGTK
                     KVPRHSNITIESSSGDEISGELLDCSSEQIVVRSSFGEVAIPLQNVKDVAFAATVNTK
                     GNAQQWPADPNARRALFLPTTETLRKGDKMYENYYLFVHNFYYGVSDQVTINAGLTAI
                     PTDGDFIDNQVYGLGIKARFYETPKIRFAAGAQFVKMPWSESEDVNKFAYTYGVLGIG
                     DPSDAQLNLSMGYILGISSSEKPFIYSGNVSKRLSRNFSLIGEVMWVGTQQDPDYSTV
                     FYSYGIRFMTRRLGVDLGFFNVSEEGYFPGIPMVNFNYSWR"
     gene            2909..3319
                     /locus_tag="PK754_10825"
     CDS             2909..3319
                     /locus_tag="PK754_10825"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNB10042.1"
                     /translation="MFSFFQRHSHIAVLFLRIGTGFVFIYHGYNKIFAPGGIERFTGY
                     LSGLGFPLPSVLAYTSGGVEFFGGCMLALGLLTRQASLLLIVNMLVAVFIAHRHDTYP
                     QKEHAIQMLVLSIGTLYSGSGAFSLENLVKKHGD"
     gene            3322..3504
                     /locus_tag="PK754_10830"
     CDS             3322..3504
                     /locus_tag="PK754_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10043.1"
                     /translation="MTYSEMKKMCEDIDYYAGHKLKPDDAYEFKKLYNRIKDDEDLDS
                     LSQEKLRKIHDTYLKK"
     gene            3516..3830
                     /locus_tag="PK754_10835"
     CDS             3516..3830
                     /locus_tag="PK754_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNB10044.1"
                     /translation="MLVVTTNNIEGKSIKTYYGVVGGEAILGANIFRDFFAGIRDIVG
                     GRSASYEEELRKAKGIAIQEMEAQAAALGANAVIGVDLDYETVGQSMLMVTAAGTAVY
                     IE"
     gene            3834..4472
                     /locus_tag="PK754_10840"
     CDS             3834..4472
                     /locus_tag="PK754_10840"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNB10045.1"
                     /translation="MFTGLIEETGKVASVIPVGQGRRFTIRASKVMDGLSIDDSVAVN
                     GCCLTVIARTSDSFDVEAVEQTLSKTTLGEFEIGTVVNLERAMQLNDRLGGHLVLGHV
                     DGVGSIVTIEERSLSHWVTIAIPAELEKYVIAVGSITLDGISLTVANLKENCISVAII
                     PHTWKVTNFAKRSPGDRINVEVDMIGKYVEKLMTRKPEEQKITAEWLLDQGK"
     gene            4475..5599
                     /gene="ribB"
                     /locus_tag="PK754_10845"
     CDS             4475..5599
                     /gene="ribB"
                     /locus_tag="PK754_10845"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HNB10046.1"
                     /translation="MHFNTIEEAVEDYKKGKIVIVVDDEDRENEGDFIMAAEKVTPDA
                     VNFMAKHGRGLICLSITRQRASELELNIMVDRNTSLHTTPFTVSIDAKEGTTTGISAA
                     DRAKTISVVIDPVTRPEDLARPGHVFPLVARDGGVLERSGHTEATVDLARLAGLYPAG
                     ILCEIMDDDGSMARVPRLMEMAKTFDLKIITIKDLIEYRRRMERFIRPVVKDVELPSR
                     YGNFSVTVYENILNGDQHIAITKGLIKGEDAILVRVQVENTMGDVFGSLTGNNSSHVS
                     DSLRMIESAGRGVVLYLRENDQRSLGLFPSEEVSSLDSHHSRDTDKDWRTLGVGSQIL
                     VDLGVRRLKLLTNNRKKYVGLEGYGLEIVEQIPIVSQAKV"
     gene            complement(5899..6363)
                     /gene="rpiB"
                     /locus_tag="PK754_10850"
     CDS             complement(5899..6363)
                     /gene="rpiB"
                     /locus_tag="PK754_10850"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNB10047.1"
                     /translation="MLKQGAERKVALGADHGGFPLKEFLKKVLIEMQFTIVDVGTHST
                     ESVDYPDFAEAVAKQVATGECFRGIMIDGAGIGSCMVANKVSGVRAAMCYDITTAVNS
                     REHNNANVLTLGSKMIGETVAEHIVKVFLKTDFAGGRHQGRIDKMMAVEKKR"
     gene            complement(6555..>6890)
                     /locus_tag="PK754_10855"
     CDS             complement(6555..>6890)
                     /locus_tag="PK754_10855"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454477.1"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HNB10048.1"
                     /translation="TASGGIGAAIEKAIEYNRQNNLHLSIEVETKNLDEVREALRYPV
                     QRIMLDNMKIETMREAVAIINKRCETEASGNVSLDTVKDIAETGINFISIGALTHSAP
                     ALDLSLLVE"
BASE COUNT         1895 a         1395 c         1701 g         1899 t
ORIGIN      
        1 ttacagcggc atgatgaaag ccggattgca gatatatttg acaggcgggg gaaccaagtt
       61 ccgcgtcccg ctgcatgaag attacaagca catgaaaaaa atctcggaag ccggtgccgc
      121 tatcgaaaaa gtaaaagccg atgaaaagct gacgtttgaa aaacttacgg atgtttatta
      181 ctccggcaca aaacatgaag aacatcagcc ttgccatttg gtgattacac cggacgatct
      241 ggcaaatatt tgtaataccc gctgcaaaga agaatatggc aatccatgcc agcacttctg
      301 ccctgcacag gtgtacaata tgatcacgcc cgaagcgggt aaaccgacac aactacgtat
      361 tgatttttcc aattgtgtac attgcaagac atgcgatatc gccgatcctt atcaggttat
      421 caattgggtt acgcccgagg gcgggggcgg gcctgtttat aagaatatgt aaacaaaaaa
      481 tgggggtttt atgaaaaagg ggaaaaagta aggatgacag atagtagtag tatgaagtta
      541 agttggttta tcctgttaat tacggtttgt atttcgaatg tcaatgcgca ggaagcacag
      601 tcgtattttg atgacggcgg taagatggca gttattgacg cagcgacaaa taccaggctc
      661 aatttattcc cgtcgtatga aggttttatt gaggcgcgtc tatttgaaac ggaagacggc
      721 gaatacatgc ttgagatttt gtatcaggcc aaccataaaa tactgcgtca acgcattcct
      781 caaaatgggg atcaggtgcg tgcgctccgt gagcgtattg cctcaggtaa aactcaacaa
      841 caagcgtcgg ttatcaatca ggaagggcgc gtgcgtttgt tatcggttgc tgccggagct
      901 tcattgggtt attatgccat agctgtaccg atcatcgcga aagttgaagg acgagccgcg
      961 gtaggattat atttactaac cgggggcctt ggtttttatg ttccttatca gattacaaaa
     1021 aacatgtcta tcactcatgg tgcggcagat ggttatacct atggtttgtt tcatggcgca
     1081 ggttacgggg ccgcattaaa ttttttagct tccggaggcg atatcacagg tcgtcaattt
     1141 ttattttcga ccagtgtcgg cagtatagcc ggaagcatat tggggtatca atatgctaaa
     1201 cgtaataatc tatcttccgg cgatgtcgcc gtgtacaata tcggcggatt atatggaacg
     1261 gggatgggac tcggcgccgc ggcgctcact gaaacaaaaa aatctcgcat ttacgcagcc
     1321 tccggtttag taggaaatat ggcaggactg gttattggtc attcgttttt gaatgcgcaa
     1381 cactatacgt ccggcgatat ggatatggtt atgaattcag gtgctttggg cgcctatctt
     1441 ttaccgtccg ttcttctgcc gactaaatta aaagataatc gcatctacat cgctgcaacg
     1501 atgaccggtg gaacacttgg tttgatgacg ggcaataatc tgatccgtgg caaggatttt
     1561 acggcttctc agtcgcgcgt aattgcactg ggcggtgcag cgggtttact gacaggtgcc
     1621 ggcatagcct atcttgttgc cccggaggat aaaccgcgtt ggtatatcgc atccagtgct
     1681 gtcggtggtt tgatcggatt ctcatggatg tatgcctcaa ataaagataa agtagaacac
     1741 gaatccggaa ataaaacatc gttaaaaatt cgatttatgc ctgaaaatta tctgatggcg
     1801 agtaaaatga aaaataagaa ggatgcgcct gctttacctg tgatgtcggc tgaattgaga
     1861 ttctgaggag ggcattaatt tacgcaatgg gggtttctgt ataatttaga gataaaataa
     1921 atatgaggga atgattatga agaatttaat ttatgtggtg gtagtttgtc tggtttttgt
     1981 atcagaagaa tcagtttata ctcaaacgtc caaaagtgat gataagaaaa cggatatggt
     2041 caatgtttgt ttgaagagcg gtacaaaaaa agttccgcgt cattccaata tcaccatcga
     2101 aagcagttcc ggcgatgaga tatcaggcga gcttttggat tgttcatccg aacagatcgt
     2161 cgtacgttca tcgtttggag aagtagcgat tcctttacaa aatgtgaagg acgttgcttt
     2221 tgccgccaca gttaacacta aaggtaatgc gcagcaatgg cccgctgatc cgaatgcgcg
     2281 ccgcgcattg tttttaccaa cgacggaaac cttgcgcaaa ggcgataaaa tgtatgaaaa
     2341 ctattacctc ttcgtacata atttttatta cggcgtttct gatcaggtaa caatcaatgc
     2401 ggggcttact gcgattccaa cggatggcga ttttatagac aatcaggttt atggcttagg
     2461 gatcaaagca cggttttacg aaacgcctaa aatacgattt gccgcgggcg cacaatttgt
     2521 aaaaatgccg tggtctgaat cggaagatgt caataaattt gcatatactt acggtgtatt
     2581 gggtattggc gatcctagtg atgcgcaact taatctgagc atgggttata ttttaggaat
     2641 ttcgagttct gaaaagccgt ttatctacag cggaaatgtc agtaaacgtc tttcccgcaa
     2701 tttttcattg atcggtgaag tgatgtgggt cggtacgcag caggatccgg attattctac
     2761 cgtattctat agttacggaa ttcgttttat gacgcgccgc cttggcgtcg atcttggttt
     2821 ttttaatgta tctgaggaag gttattttcc ggggataccg atggttaact ttaattatag
     2881 ttggcgataa caaaatacag ggctcatcat gtttagcttt ttccaacgtc actctcatat
     2941 tgccgtttta tttcttcgca tcgggacagg tttcgttttt atctatcatg gatacaataa
     3001 aatattcgca cccggcggga tcgaacgatt cacaggatat ctttcaggtt tgggttttcc
     3061 tttgccatct gtgttggcct acacgtcggg cggggtagaa ttttttggag ggtgcatgct
     3121 ggcgctggga ttgcttacac gccaggccag cctgttgctg atcgtcaata tgctggtcgc
     3181 cgtttttata gcacatcgcc atgataccta tcctcaaaag gagcatgcga tacaaatgct
     3241 ggttctttcg atcggtacgc tctacagcgg cagcggcgca ttctcgttag agaatctggt
     3301 taaaaaacac ggagactaaa catgacgtac agtgaaatga aaaaaatgtg tgaagatatt
     3361 gattattacg ccggtcacaa attaaaacct gatgacgcgt acgaattcaa aaaattgtat
     3421 aaccgaatca aagatgacga agatctggat agtttgtcgc aggaaaaatt gagaaaaatt
     3481 catgatacat atcttaaaaa ataaggaagc gtactatgct ggttgtaacc accaataaca
     3541 tcgaaggcaa atccatcaaa acatattacg gcgtcgtcgg aggggaagct atcctcggcg
     3601 cgaatatttt ccgtgatttt tttgcgggaa tacgcgacat cgtcggcgga cgttcggcat
     3661 cgtatgaaga agaattgcgc aaagccaaag gtattgcaat acaagaaatg gaagcgcaag
     3721 ccgcggcgtt gggtgcgaat gccgtgatcg gagtagatct ggattatgaa accgtcggtc
     3781 aaagtatgct gatggtcact gcggcgggca cggctgttta catcgaataa tttatgttca
     3841 caggacttat tgaagaaacg ggtaaagtag cctccgtcat tccggtgggg caagggcgtc
     3901 gttttacgat ccgcgcatct aaagtcatgg atggattatc catagatgac agtgtcgctg
     3961 tcaatggatg ttgcctcacg gtgatcgcgc gaacgagcga ttcgtttgat gtggaggcgg
     4021 tagaacagac gttatccaaa accacacttg gcgaatttga aatcggtacg gtagttaatc
     4081 tggaacgtgc gatgcaactc aacgatcgtt tgggcggcca tttggtactg ggacacgtgg
     4141 acggtgtagg ttctatcgtg accatcgaag agcgttcttt gagtcactgg gtaacgatag
     4201 ccataccggc cgaattggaa aagtatgtta ttgcggtagg ttcgatcacg ctggatggca
     4261 tcagccttac ggtggcgaat ttgaaggaga attgcatatc tgtggcgatc ataccgcata
     4321 cttggaaagt aaccaatttc gccaaacgat ctcccggcga ccgaatcaat gtagaagtgg
     4381 atatgatcgg aaaatacgtg gagaaattaa tgacacgaaa accggaagaa cagaaaatca
     4441 cagccgaatg gctcctggat cagggaaaat agatatgcat tttaacacca tcgaagaagc
     4501 cgtagaagat tacaaaaaag gtaaaatcgt catcgtagta gatgatgaag accgcgaaaa
     4561 tgaaggcgat tttatcatgg ccgccgaaaa ggtcactccg gatgcggtta attttatggc
     4621 gaaacacggc cgtggtttga tatgtttgtc cataacgcgt caacgcgcct cagaacttga
     4681 gctcaatatc atggtggatc gcaacacctc attgcacacg actccgttta ccgtgagcat
     4741 tgatgcaaaa gaaggcacga caaccgggat atcagctgca gatcgtgcaa aaactatttc
     4801 cgtagtgata gatccggtga cgcgaccgga agaccttgca cgtcccggcc atgtttttcc
     4861 tttggtggca cgtgacggcg gcgtattgga acgctccggt cataccgaag cgaccgtaga
     4921 tcttgcacgc cttgcgggac tttatccggc aggaatacta tgcgaaatca tggatgatga
     4981 cggttcgatg gcgcgtgtac cgaggctgat ggaaatggct aaaacttttg atctcaaaat
     5041 aattaccatt aaagacctga tcgaataccg tcgccggatg gaacgcttca ttcgccccgt
     5101 agtcaaagat gtcgaattac cgagccgtta cgggaatttc tccgttacgg tgtatgaaaa
     5161 catactcaac ggcgatcagc acattgcaat aaccaaaggt ttgatcaaag gcgaggatgc
     5221 gattctagtt cgtgtgcagg ttgaaaatac gatgggtgac gtgtttggtt cattgaccgg
     5281 gaataattca tcgcatgtat ccgacagctt acgcatgatc gaatcagccg gacggggcgt
     5341 cgtgctgtat ctgcgtgaga atgatcagcg ctcgctcgga ctttttccat ccgaagaggt
     5401 atcatcttta gacagtcatc attcgcgtga tacggataaa gattggcgta ccttgggtgt
     5461 cggatctcaa atattggttg atctgggggt gcgccgcctt aaacttttaa ccaacaatcg
     5521 aaaaaaatac gtcggacttg aaggttatgg gttggaaatc gtggagcaga ttccgatcgt
     5581 atcgcaagcc aaagtttaaa ccaatctttt ttgaatatgt ttctctcgcg gtagtaactc
     5641 agatttcccg taactcccga taggatacag cttagctaat cttctacatg tcttaccaaa
     5701 tgaaaatctt gatataattt acgtattttc tgagaagttc ttttaagaaa gagtgtagtg
     5761 aaaacagcgt cacgtattca aacgacggtc aatagcgcag tagggcaggg cgagatgaag
     5821 taatgcattt caaaaggatt taataaattc tatcggaagg gaaaaataaa acgatttggt
     5881 ttttatatct acagcagctt accgcttctt ttcaacggcc atcattttat caatacgtcc
     5941 ctggtgcctt cccccggcaa aatccgtttt gagaaaaact ttaacaatat gttcagcgac
     6001 ggtttcgccg atcattttag agccgagcgt taagacattg gcgttattat gttcacggct
     6061 gttgacggct gtcgtgatat cgtaacacat cgctgcccgt acgccggata ctttattggc
     6121 taccatacat gaaccgatac ctgcaccgtc aatcataata ccgcgaaaac actcaccggt
     6181 tgcgacttgt tttgccacgg cttctgcaaa atccggataa tctaccgatt ccgtcgaatg
     6241 ggttccaaca tcaacgatgg tgaactgcat ttcaataagt acttttttaa ggaattcttt
     6301 taacggaaat ccaccgtgat ccgcgcccaa ggctacttta cgctctgcgc cttgtttgag
     6361 cagtagattg gaaactgtag atggggcaac ggtttgcggc ttaacgattt gaaatgtcgc
     6421 acctaactgt ttggctttat cacgtgctaa tgccgtgatc aaagcgtccg aggagatcat
     6481 gatcgttttc tgtccgcttt tgacggtttc gatgactgta cgttcagtta ttaattttcg
     6541 tttcatcgta atgattattc tactaaaagt gaaagatcaa gcgccggcgc cgagtgtgtc
     6601 agcgcgccta tggaaataaa attaatgccg gtttcggcga tatcttttac cgtatcaagg
     6661 gaaacattac cggatgcttc ggtttcacat cttttattga ttattgcaac agcttcacgc
     6721 atggtttcga tcttcatatt gtcaagcatg atgcgctgga ccgggtaacg caatgcttcg
     6781 cgcacttcat ctaagttttt ggtttcaact tcgatggaca aatgtaagtt attttggcgg
     6841 ttgtattcaa tggctttttc tatggctgcg ccgatacctc cggacgccgt
//
LOCUS       DAOQNQ010000179         6834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_4255_length_6834_cov_5.961056, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000179 DAOQNQ010000000
VERSION     DAOQNQ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4255_length_6834_cov_5.961056"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2958
                     /gene="mfd"
                     /locus_tag="PK754_10860"
     CDS             <1..2958
                     /gene="mfd"
                     /locus_tag="PK754_10860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNB10049.1"
                     /translation="LQSGATFQFQYLVELLHEIGFEKQEIVDQPGTFAIRGGIVDVFP
                     FAQQNPIRIEFFGDTIDSLRFFDVVNQRSLQKTDYLALYPKSESQTAQHDADLTDYWP
                     QDTLVMLLEPEILQGALDKYWDEINDSYQRKSEAHSELLAPESRFLDTASVMKKLNAF
                     ACLYFARYSRIMDFDKINFTAHALESFNSHINLLKERLIAIRGQYKVFIACNNTGQAE
                     RLSELLDSDELASGVDFVIGVGELHEGFYTEDAQCVVFTDHQIFGRIKRTRSHRKFKT
                     AQALRHIASLKPGDYVVHVDYGIARYAGLEKVTSGEHTEECLKLLYKNGDKLFVPLEH
                     FSRVQKFSAEDGVTPTLHKLGSGDWDKVKARTKKTLKDIAQDLIKLYAERKSRKGFAF
                     TPDTHMQYALEASFEFEDTPDQTKVTLDVKKDMESDNPMDRLVCGDVGYGKTEIAIRA
                     AFKAVQDSKQAAILVPTTILAEQHFETFSSRMREFPVRIDVISRFRTTKEQKIILEKV
                     QRGEIDILIGTHRLLSKDVQFKDLGLLIIDEEQRFGVAHKEKLRQLKATVDTITLTAT
                     PIPRTLHFSLMGGRDLSVINTPPQDRLPVKTEITQFDEDLIHDAVMKELDRGGQVYYV
                     HNRIQSIDRITGMLNTVVPKARLAVIHGQMAPQQVEDIVHAFMKREYDILVATTIIEN
                     GIDIPNVNTIIIDQAHQFGLSQLYQLRGRVGRSNRQAYCYLLTMPMSVMPSDALRRLQ
                     AIEEFSDLGSGFLIAMRDLEIRGAGNMLGAEQSGFVNAVGFELYCQILDEAVQETRQE
                     MNVVESGKKEEITQQAEYIPALQVHVFCDTYIPDTYVNVPSERVRIYKSLSDIISIDA
                     LNAIEQELVDRFGKLPVEAKNLLQMIEIKLMTLPLCAERLDIDENKLVLRFDVKRMSQ
                     PNVADRMKLKMNSIVKKFENLRFFQNKDFLDITFGFEWYDATKSRIISTESKEDSLVL
                     ISAKKIISQIIEL"
     gene            2978..4750
                     /locus_tag="PK754_10865"
     CDS             2978..4750
                     /locus_tag="PK754_10865"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNB10050.1"
                     /translation="MYFSKIQMYSWVLLITVMTIACKNDEKKTDLNDLRTVLAVVGED
                     TVFLGDYKNRVSELKQDKPDLESEIIRRQILDEMVNYYLVVMEGRSQKLQDHPYVRFQ
                     VRTKEDEFYFAHAIRNAIVEPSITEDQIKARYEQLKEQLDVKHIFIGYDKTFDPSLEM
                     APNQKKIIRFKLAAKSIADSLLGVLKKEPDKFENFIEQFSDDAATKFFDGTIKGLRRG
                     ELPNEIGDALFKLQPDELSQPIDDGNGYHIYKIIQKSNDANVLPYEKAKIAIKEQIAK
                     QILLKPDIQIEKNKKEFIENRLRDGSYSFNQSNVAAWYSKFKKYDSKKPLVDQLTDDE
                     LNTILATSLTDTIKIVDIAFALTGNRFNSRITIEQMESGIRNAIAVRLVSRWAKEQKI
                     ELNDREKRFIKRIESGLISTKVQPPSVDIQLIEDTSNVRHYYEANIKNYKMPDSLDLG
                     MIVMNEEAQIRPIADKIKSTRNFEISFEELRKKNPASAVRSGLRPLKEFGNLSARIQE
                     LKEGDISDVFIMNDGHYGIVKIYKRTQPSWYSLDKVFVQVKSDYSVSLIKQTRDKWLL
                     SLKEKYTPTVNYHSLKDAFEIKLK"
     gene            4786..>6834
                     /locus_tag="PK754_10870"
     CDS             4786..>6834
                     /locus_tag="PK754_10870"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB10051.1"
                     /translation="MSQINAQDHILLDSVDALLRESKYADAIHLFEVNSISTIHKPAT
                     HAALSKAYFYLGQNKESQSLYSSLLLRKDVATMDRFRWTQDYILVAAETGNFKLCDSL
                     LTIIQSILPTVKDSTALARYWNTVGIIYIQSSQPFLANEALNKAEHYMHRDLDLKLQS
                     WIVSNRGIVYKMMGDFASAIEKYRMALYQDSLRNDKADMAADYQNLSLIFSEMQLYAD
                     SRYCFEKALEYIRMTGDVGTEGIILNNMAHLFNNHGQIKSALQCLRSSLVITEKAGDK
                     IATADAYYELAGAFRALSQYDSAIYFWNKAYDIYCEIGSIDQTFFTLLEKFRAMVLLK
                     QFKQGDQLLEQAFNLLSQLPSKRYSPSYYDARALYFESINQTDSADAYYVLALQSIDS
                     IYQEQNSAHVYDASIPNDRTRFYKNYIRFLFDQKKYHEALSYQLLLNSRSTHNTKPHP
                     ILFFESGVSFFQADTVMHVLLNTERELQYRRLCITPLIKDHLLQIKTLLRQPSANQHS
                     LSQHLAVLSDWINPAIIDYVQSHKELVIVPDGLLCNFPFEMLSLERRYLTETHTIQYT
                     AALNSDEKFSIYSERTIPSVTILARSEYSSWTSLPDLPDVDNEIDRIKSIIPHSQIYY
                     QVTEKSYYDIIGTDSSQVLHLIGHGMNATDDARKSGIILGRDQIHHDGYLTCNEIEKQ
                     Q"
BASE COUNT         2148 a         1270 c         1419 g         1997 t
ORIGIN      
        1 cttcagagcg gcgccacgtt tcagtttcaa tacttggtcg aattgctcca tgagatcggt
       61 ttcgaaaagc aagaaatcgt ggatcagccg ggaacgtttg ccattcgtgg tggtatcgtt
      121 gacgtatttc cttttgctca acaaaatcct atccgtatcg aatttttcgg cgatacgatt
      181 gattcgcttc gtttttttga tgtagtaaat cagcgttcgt tgcaaaaaac agattattta
      241 gctctgtatc ccaaatcgga aagtcaaacg gcacaacacg atgccgatct gacggattat
      301 tggccacagg acacactggt catgttatta gaaccagaaa ttttgcaagg cgcattggat
      361 aagtattggg atgaaatcaa cgatagttat cagcgtaaat ccgaagctca ttcagaactt
      421 ctagccccgg aaagtcgttt tcttgatacc gcgtccgtaa tgaaaaaact caatgccttt
      481 gcgtgtcttt attttgcccg ttatagccgt attatggatt ttgataaaat aaattttacg
      541 gctcatgcac tcgaatcatt taacagtcac atcaacctgc tcaaagaacg attgatagcc
      601 attcggggcc aatataaggt tttcatagcg tgtaataata cgggtcaggc tgaacgatta
      661 tccgaattgt tggattccga tgaacttgcg tccggcgttg attttgtaat cggcgtcggc
      721 gagttgcatg aaggatttta cacggaagat gctcaatgtg tcgtatttac ggatcatcag
      781 atttttggac gcatcaagcg tacgcgatca catcgcaaat ttaaaacagc gcaggcgtta
      841 cgtcacattg catcgctcaa accgggagat tatgtagttc atgtggatta cggtattgcg
      901 cgctatgccg gtcttgaaaa agtaaccagc ggtgaacata ccgaagaatg tttgaaactt
      961 ctttataaaa acggtgataa attatttgtc ccgctcgaac atttttcacg cgtccaaaag
     1021 ttttcggctg aagacggcgt tacaccgacg ttacataaat tgggttcagg cgattgggat
     1081 aaggttaaag cacgcaccaa aaaaacgctg aaagatatcg cacaggatct gatcaaactc
     1141 tatgccgaac gcaaatcacg caaaggattc gcttttactc cggataccca tatgcaatac
     1201 gcattggaag catcgttcga atttgaagat acgcccgatc aaaccaaggt gacgctcgac
     1261 gtcaaaaagg acatggaaag cgataatccg atggataggc ttgtctgtgg tgatgtgggt
     1321 tacggtaaaa cggaaatcgc tatacgtgct gcttttaaag ccgttcagga tagtaagcaa
     1381 gcggcgatac tcgttcccac gacgattttg gcggaacagc attttgaaac gttttcttca
     1441 cgtatgcgcg aatttccggt tcgcattgat gtgatatctc gttttcggac aaccaaagaa
     1501 caaaaaatca tcctcgaaaa agtacagcgc ggcgaaattg atatactgat cggcacgcac
     1561 cgacttcttt caaaggatgt gcagttcaaa gatttaggcc ttcttattat tgatgaggaa
     1621 cagcgcttcg gcgttgctca taaggaaaaa cttcgccagc tcaaagctac cgtcgatacg
     1681 atcactctga cagcgacgcc gataccgcga acattgcact tttcgttgat gggcggacgt
     1741 gacttgtcgg tcatcaatac gccaccgcag gatcgtttac ccgttaaaac agagataacg
     1801 cagtttgacg aagacctcat ccatgatgcc gtgatgaaag aattggaccg gggtggacag
     1861 gtttattatg tgcacaatcg gatacagagt attgatcgca tcacgggcat gctcaatacg
     1921 gttgttccaa aagcacgatt agctgtgatc cacgggcaaa tggcaccgca acaagttgag
     1981 gatatcgttc atgctttcat gaaacgcgaa tatgatattt tagttgctac gacaatcatt
     2041 gaaaacggta ttgacatacc aaacgtcaat actatcatca tagaccaggc acatcagttt
     2101 gggttatcgc agttatatca attacgcggt cgggtcggtc gctccaatcg tcaggcgtat
     2161 tgctatcttc tcacgatgcc catgagtgta atgccttcgg atgcgttgcg acgattgcaa
     2221 gcgatagagg aattctcgga tctgggttca ggttttttga ttgctatgcg ggacttagaa
     2281 attcgcggtg ccggcaatat gcttggcgct gaacaaagcg gttttgtaaa tgcggttggt
     2341 tttgaacttt attgtcaaat tttggatgaa gccgtacaag agacacgcca agaaatgaat
     2401 gtggtcgagt cgggtaaaaa agaggaaata actcagcaag cagagtatat tccggcttta
     2461 caggttcatg ttttttgcga tacctatata ccggatactt atgttaatgt tccatctgaa
     2521 agggtcagga tttataaaag cttatcggat atcatttcaa ttgatgcttt aaatgcaatc
     2581 gaacaagaat tggtggaccg ttttgggaag cttccagtag aagctaaaaa tcttctgcaa
     2641 atgatcgaaa taaagttgat gacattgccg ctttgtgctg aacgattgga cattgatgaa
     2701 aacaaattag tacttcggtt tgatgtgaaa cgcatgagtc aaccgaatgt ggcagatcgc
     2761 atgaaattaa aaatgaattc tattgtaaaa aaattcgaaa atcttcgatt ttttcagaat
     2821 aaggactttt tagatattac ttttggtttt gagtggtatg atgcgacaaa aagccgtatt
     2881 atttcaactg agtctaaaga agattcttta gtattgataa gtgcaaaaaa aataatttca
     2941 caaataattg agttataaat cattcataag gttaaatatg tatttttcta aaattcaaat
     3001 gtactcgtgg gtgctattga ttacggttat gaccatagcc tgtaaaaatg atgaaaaaaa
     3061 aaccgatttg aatgatttga gaacagtatt agcggtagtc ggagaagata ccgttttttt
     3121 aggtgattat aaaaatcggg tttcagaatt gaaacaggat aaaccggatt tagagtcgga
     3181 aattattcga cgtcaaatcc ttgatgagat ggtaaactat taccttgtgg tcatggaggg
     3241 gagatcacag aaattacagg atcatcctta tgtgcgtttt caagttcgta ctaaggaaga
     3301 cgagttttat tttgctcatg cgatacgaaa tgcaattgta gaaccctcaa taacagaaga
     3361 tcaaattaaa gcgcgatatg aacagctaaa agagcagttg gatgtaaaac atatctttat
     3421 cggatatgat aaaacttttg atccttcttt ggaaatggca cccaatcaaa agaaaatcat
     3481 acgatttaag ctggccgcca aatccattgc tgattctttg ttgggcgtac tcaaaaaaga
     3541 acccgataaa tttgaaaatt ttatagaaca gttttctgac gatgcagcta caaaattttt
     3601 cgatggcact atcaaaggct tacgtcgagg tgaattgcct aatgaaatag gcgatgcttt
     3661 gttcaaactg caacccgatg aattatctca accgattgac gacgggaacg gttatcatat
     3721 ttataaaatc atacaaaaaa gcaatgatgc taatgtgttg ccatatgaaa aagcaaagat
     3781 tgccattaag gagcaaattg ctaaacaaat tcttttaaaa cctgatatac aaatcgaaaa
     3841 aaataaaaaa gagttcatcg aaaatcggct acgtgacggg tcatacagtt ttaatcaatc
     3901 caatgttgcg gcatggtatt ctaaattcaa aaaatatgat tccaaaaaac cattggttga
     3961 tcagttaacc gatgatgaac taaatacgat cctcgctaca tcactgacgg atacaataaa
     4021 aatagtggat atagccttcg cactcaccgg caatcgcttt aattcacgta ttacgataga
     4081 acagatggag tccggtattc gcaatgccat agctgttaga cttgtatcac gttgggctaa
     4141 agaacagaaa atcgaactaa atgatcgcga aaaacgtttt atcaagcgaa tagaaagcgg
     4201 attgatctct acaaaagttc aaccgccttc agttgatatt caattgatcg aagatacatc
     4261 taatgttcgt cactattatg aggcaaatat aaagaattac aaaatgccgg acagtttgga
     4321 tttgggaatg attgtaatga acgaagaagc gcagatacgg cctatcgcag ataaaattaa
     4381 atctacaagg aactttgaaa taagttttga agaattgcga aagaaaaacc cggcatctgc
     4441 tgtgcgttcc ggtctgagac ctttaaagga atttgggaac ttgtcagcgc gtatacaaga
     4501 actcaaagaa ggtgatataa gtgacgtctt tattatgaac gatggccatt acggtatagt
     4561 taagatttac aaaagaacac aaccgagctg gtattcattg gataaagtgt ttgttcaagt
     4621 taaatctgat tattcagttt cattaatcaa acagacacgt gataaatggt tattaagctt
     4681 aaaagaaaaa tatacgccta cagttaatta tcactcgttg aaggatgctt ttgaaattaa
     4741 gctgaaatga tttttatgag attgtttctt tggtttctgt ttgttatgag ccaaataaat
     4801 gctcaggatc atatattact tgatagtgtg gacgccttac ttcgtgaaag taagtatgcc
     4861 gatgccatac acctttttga agtaaatagt atttccacaa tacataagcc cgctacccat
     4921 gctgcgctgt cgaaagcgta tttctatctt ggtcaaaaca aggagtcaca aagtttgtat
     4981 tcatcgctgt tactacgtaa ggatgtagca acaatggatc gttttcggtg gactcaggat
     5041 tatattctcg tagctgctga aacaggtaat tttaaactct gcgactcgtt actcactata
     5101 atccaatcaa tacttcctac ggttaaagat tcgaccgcct tggcccgcta ttggaatacg
     5161 gttggtatca tctatatcca atcttctcaa ccctttttag ccaatgaagc tttgaataaa
     5221 gctgagcatt acatgcaccg cgacttagat ctcaaattgc aatcgtggat tgtttccaat
     5281 cgtggcattg tttataaaat gatgggggat tttgcttccg caatagaaaa atatagaatg
     5341 gctttgtacc aagattctct gcgtaatgat aaggccgaca tggcggctga ttatcaaaac
     5401 ttgagtttga ttttttcaga gatgcaatta tatgctgaca gccgttattg ttttgaaaaa
     5461 gcacttgaat acatacgaat gaccggagat gtaggaaccg agggaataat tttgaacaat
     5521 atggcacatt tatttaataa ccatggccaa ataaaatcag cccttcaatg tttacgaagc
     5581 tcattggtta tcactgaaaa agcaggtgac aaaattgcaa cggctgacgc ttattatgaa
     5641 ttagccgggg catttcgcgc gttatcgcaa tatgacagcg ctatatactt ttggaataaa
     5701 gcatatgata tttattgtga aattggttcg atagatcaga catttttcac ccttcttgaa
     5761 aaatttcgtg cgatggtgct gttaaaacaa tttaaacaag gggatcaatt gttagaacaa
     5821 gctttcaatt tgttgtccca acttccttca aaacggtata gcccaagtta ttatgacgcg
     5881 cgtgctttat actttgaatc aattaaccaa acggatagtg ctgatgcata ttatgtcctt
     5941 gcgctgcaat ccatagattc gatctatcag gaacaaaatt ctgcacatgt atatgatgcc
     6001 agtattccca atgatcgtac tcgattttat aaaaactata ttcgatttct ttttgatcaa
     6061 aaaaagtatc atgaagcatt gagctatcag cttttattaa acagtcgctc tactcataat
     6121 actaaaccgc atccaattct tttttttgaa tccggcgttt cattctttca agctgataca
     6181 gtcatgcatg ttttactcaa taccgagagg gaactgcaat atcgtcgttt gtgtataaca
     6241 ccacttatta aggatcacct tttacaaatt aaaacccttc ttcgacaacc ttccgctaat
     6301 cagcatagtc tatcacagca tcttgctgta ttatcggatt ggataaatcc agcgattata
     6361 gattacgttc aatcacataa agaattggtc atagttcctg acggtttgtt gtgtaatttt
     6421 ccttttgaga tgttgagctt agaacgccga tatctgactg aaacgcatac gatccaatat
     6481 acagcagcgc taaattctga tgaaaaattt tcgatctatt cggaacgaac aataccgtcg
     6541 gtcactatat tggcccgctc ggaatatagt tcgtggacgt cattgccgga tctgccggat
     6601 gtagataacg aaatagatcg catcaagtct attattccgc atagccaaat ttattaccaa
     6661 gttactgaaa aaagctatta tgatatcatt ggcacggatt catcacaagt tttgcatttg
     6721 ataggtcatg gtatgaatgc gacagacgat gcccgtaaaa gcggcattat attagggcgt
     6781 gatcaaattc accatgatgg atatttgacg tgtaatgaaa tcgaaaaaca acaa
//
LOCUS       DAOQNQ010000180         6800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_4294_length_6800_cov_6.002817, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000180 DAOQNQ010000000
VERSION     DAOQNQ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4294_length_6800_cov_6.002817"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1897)
                     /locus_tag="PK754_10875"
     CDS             complement(<1..1897)
                     /locus_tag="PK754_10875"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB10052.1"
                     /translation="MKRIIALVLSLCAAVSASAQWPRLSVKTAVTAIAVEDTVTWIGT
                     NDGIRGVVNGTEKFYFDKGNGLTSNIIRAITIDNQNRKWIATEEGLNVLDGKKVKQYL
                     KSPETLPTNEINSVHADEEGSVWIGTEKGLVRISGNSWTLLDRKTTLDGLPSNTIRGI
                     SSVWNGPVWIATDDGLVSYYNKKWALYTRKLNNLPSDDVFDVVADPSSGNVWIGTSRG
                     VALFDGKLMTQFLSDIPVRALTLDKIGRCWVATKNEGVHLIDGARVVKYSTKEGMLVS
                     DEGYAVTCDAFNTIWAGHQLGVQTFLDETIGSRIAPYFLTEAQKAFWSNDNTRARLYY
                     NHIVNRSYLSATPEAATAMIGLGELYEREAMIERAADTYLRFTERFPNDARTKNLLLK
                     SAQLQSQIKKYAESAATYQKFLTLYKDDADAFTIAWMRYNLLELAGDSVSALQTLQNL
                     NTQFPSNPRADEIRWRILSAGNSAGVASKELRALANSTRDFEVQYQLAALYDTQHRKD
                     ILEGLQGSITWQTLFSPSAVTSIFEDGDIAWASTSGNGVVKWDIKLNALTSYSDGLKT
                     PTVKSFYMDADRDLWAMIEGVPESNLYNMNYSKNRYKWIIQPAPFAKKTINQVLYRAK
                     TKTTICAT"
     gene            2016..3185
                     /gene="aroC"
                     /locus_tag="PK754_10880"
     CDS             2016..3185
                     /gene="aroC"
                     /locus_tag="PK754_10880"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNB10053.1"
                     /translation="MIRWLTSGESHGSGLVGIIDGVPAHLKLSVDVINHQLWRRQQGY
                     GRGGRMKIETDQAEIISGVRFGETIGAPIGLRIRNNDWENWTDKMMTETPSPEILKTV
                     KPVTLPRPGHADLAGIQKFGFDDIRPVIERSSARETAMRVALGTVAREVLLSCGIRIA
                     SHVTHIGRASLTAAESEKLRVDTMTTEAINQKADLSSVRVLDAEAEQRMIAVIKETQK
                     QGDSLGGIFEVYADGLPAGIGSYTHWDKKLDGVIAQHMASIPAIKGVEIGNAFDNALQ
                     WGSEVHDAIFYDDVRGYYRKTNRSGGLEGGMTTGERIIVRCAMKPIPTLARPLMSVDI
                     RTHESASAHKERTDTVSVPACSVVAESMLALALINPLLEKYGGDSMNDIRTAMGR"
     gene            3380..4276
                     /gene="xerC"
                     /locus_tag="PK754_10885"
     CDS             3380..4276
                     /gene="xerC"
                     /locus_tag="PK754_10885"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HNB10054.1"
                     /translation="MIRQHLHDFLKYLDAERNFSDKTVGAYHTDVAAFISFVETRFDS
                     SSFDASDISKNEIRAYLGQLSRDRLQKKSIARKLSAVKSFFKYLTKQQIITANPAKLI
                     ATPKFERKMPAFLGQEQVRDVFDYVDAGTPEGLRDRAILELFYGCGIRLSELTGLNFS
                     DINFTGGTLSVFGKGAKQRIIPMGKHAGAALQSYIDYRRSVSSADKNAVFLGKNGKRI
                     TPLAVQRLVKRALEKVSDAKKLSPHILRHSFATHLLDNGADLRAVKDLLGHENLSTTQ
                     VYTHVTVDRLKKVYEKAHPRAK"
     gene            4370..4705
                     /gene="raiA"
                     /locus_tag="PK754_10890"
     CDS             4370..4705
                     /gene="raiA"
                     /locus_tag="PK754_10890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNB10055.1"
                     /translation="MKIRITARHQKMSPKVKAYIEEKLEKLDRYHDHIIDCEVIVDKE
                     KLKEVVEVNVKVFGKVINVKAKDADVTKATDLAMDKLESQIKKFNDKIKDKKKKSVKK
                     MPIIDEDEE"
     gene            4818..6422
                     /locus_tag="PK754_10895"
     CDS             4818..6422
                     /locus_tag="PK754_10895"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNB10056.1"
                     /translation="MANLNQSYDFIKQIGVGGMATVFLGRHPALERLVAIKVVKGEQK
                     DKVRRFEREARLSATLKQENLPAIFDYFTDDQNNHYLVMEYVDGVDISPIIKSKTTIP
                     PMIVAMIAREVCRGLEHMHENGIIHRDIKPSNVRLGKNGQVKLMDFGIAKHEEDAGKS
                     HLTATGVIVGTPSYMSPEQASGDQLTPQSDVYSLGIMMYEMLTGKKPYTADTNMTLIS
                     LIAQGKFTPLSETHPHLPPALVDIVHRAMVKSLRQRYATTSEIIKDLNKYLQSMSQIE
                     IRDWLVRYYEAATTKDKIVDMNVFVLPAPVSVETAETSTRPVDTEKNNNFVLNKRHLV
                     LGSAALVVLCVLAYLFWPSDHPLGNYDPYGRLALSVRSDRQTIKDTRVYVNDSEFPLD
                     DNFGGSLIIDNLQPGRNAIKIRFPLLYHTYEYNFTLDGADESKALNLDLTKIAASLDG
                     VRPESRRYGFAVISEPAGVSVHLDDLKSKPLGKSPTGLIFPSVKTGLHKIFLQKEGYV
                     TSAIERNFTPDQNYTLQYELGPAKKK"
     gene            6419..>6800
                     /locus_tag="PK754_10900"
     CDS             6419..>6800
                     /locus_tag="PK754_10900"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNB10057.1"
                     /translation="MIETTDIPILRKGDDVTLDIETVTFGGQGVARYHDFVVFVEGAI
                     TGDRVQARIMKKKKNFAEARSLQIIEPSRFRVDHACTHYGICGGCKWQDVDYDAQLEF
                     KRRNVIDVFERIGGFKGLDIPAPLG"
BASE COUNT         1804 a         1554 c         1677 g         1765 t
ORIGIN      
        1 cggtagcgca aatcgtagtt ttagttttag cccgataaag tacctgattt attgtctttt
       61 tcgcgaaggg cgcgggttgt ataatccact tgtagcgatt cttgctgtaa ttcatgttat
      121 acaaattact ctcaggcacg ccttcgatca tagcccacag atcacgatct gcatccatgt
      181 aaaacgattt taccgtcggt gttttcagtc cgtcgctgta ggaggtgagt gcgttgagtt
      241 tgatatccca tttgacaaca ccgttgcctg atgtggacgc ccaggcaatg tcgccgtctt
      301 cgaatatgga ggtgacggca gaaggtgaaa acagcgtttg ccatgtgatg ctgccttgca
      361 ggccttcgag tatatctttg cggtgctggg tatcataaag cgccgcaagt tggtactgta
      421 cttcaaagtc acgtgtcgaa ttagctaagg cacggagttc tttagatgcg accccggcag
      481 agtttccggc ggagagtatc cgccagcgaa tttcgtcggc acgcggattg gatgggaatt
      541 gtgtgttaag attttgcagc gtttgaagcg cactcaccga atccccggcg agttctaaga
      601 ggttataacg catccacgca atggtaaaag cgtccgcatc atccttgtag agggtgagaa
      661 atttctgata cgtagcagcg gattcagcat atttttttat ctgtgactgc agttgtgccg
      721 atttcaaaag taaatttttt gtacgtgcat cgttcggaaa gcgctccgta aaacgaagat
      781 aggtgtcggc ggcacgttcg atcatagctt cgcgctcgta gagttcgccc aacccgatca
      841 ttgcggtcgc ggcttccggt gttgcggata agtacgaccg gttgactata tgattatagt
      901 ataaacgggc acgcgtattg tcgttggacc aaaatgcttt ttgcgcctcg gtaagaaaat
      961 aaggagcgat gcgactccct atggtttcat ctaaaaaagt ttgaacgccg agctggtggc
     1021 ccgcccatat cgtattgaac gcgtcgcacg tgacggcata tccttcgtcg gataccagca
     1081 ttccttcttt agtcgaatac ttcacgacac gcgcgccgtc gatcagatgc acaccttcat
     1141 ttttggtggc gacccagcag cgtccgatct tatccaaagt cagggcgcga accggtatat
     1201 cggagagaaa ttgcgtcatc aatttcccgt caaataaagc gacgccgcgt gaagttccga
     1261 tccacacgtt gccggaggag gggtccgcga ccacgtcaaa tacgtcatcg gacggaaggt
     1321 tattcagttt gcgtgtatag agcgcccatt ttttgttgta gtatgaaacc aagccgtcat
     1381 cggttgcgat ccaaaccgga ccattccaaa ccgaactgat tccgcgaatg gtgttggacg
     1441 gaaggccgtc caaagtcgtc ttgcgatcta aaagcgtcca actgttgccg ctgatacgta
     1501 ccaatccttt ttccgtgccg atccacacac taccttcttc atcggcgtgg acactgttga
     1561 tttcgttggt cggcaaagtt tccggactct tgaggtattg tttgactttt tttccgtcaa
     1621 gtacgttaag gccttcttcc gtcgcgatcc attttcggtt ttggttgtcg atcgtgatag
     1681 cgcggatgat attggacgtc agcccgttgc ctttgtcaaa ataaaatttc tccgttccgt
     1741 taacgacgcc gcgaatacca tcgttggtgc cgatccaggt taccgtatct tccacggcaa
     1801 tggccgttac agctgtttta accgataaac gcggccactg ggcggaggcc gataccgcag
     1861 cacacagtga taaaactaat gcaataatcc tcttcataga ctgtattttc ctcataagaa
     1921 ttcgtttttt tagctgtttt aattatacct tttttggcct ataattgcaa ttcataacac
     1981 atgtttgcga gtttaatttt ttagggaatc cttatatgat acgttggttg acgtcaggcg
     2041 aatcgcatgg ttcgggattg gtaggcatta tagacggtgt gcctgcgcat ctgaagttgt
     2101 cggttgatgt catcaatcat cagctttggc gccgtcaaca gggctatgga cggggcggtc
     2161 gcatgaaaat cgaaaccgat caggcggaga ttatttccgg tgttcgtttt ggtgaaacca
     2221 tcggtgctcc gataggattg cgcattcgca ataacgactg ggaaaattgg accgacaaaa
     2281 tgatgacgga aactccatcg ccggagattt tgaaaaccgt caagccggta actctcccca
     2341 gaccgggaca tgccgatttg gccgggatac aaaaatttgg ttttgatgat atacgtccgg
     2401 tgatcgaacg atccagcgcc cgtgaaacgg caatgcgtgt tgcgttgggg accgtggcgc
     2461 gtgaggtttt attatcgtgc ggtattcgta tcgcgtcgca cgtgacgcat atcgggcgcg
     2521 catctctgac cgcggctgaa tcggagaaac tccgcgtgga tacgatgacg acggaggcga
     2581 ttaatcaaaa agccgatcta tcctccgtac gggttttgga tgccgaagcc gaacaacgta
     2641 tgatcgccgt catcaaagaa acgcaaaaac aaggcgactc actcggtgga atttttgaag
     2701 tgtatgcgga tggtttgcct gcgggaatcg gcagttatac gcattgggat aaaaaattag
     2761 acggcgtgat cgcgcagcat atggcttcga taccggcgat taaaggtgtg gaaatcggaa
     2821 acgcatttga taacgccttg caatggggtt cggaagtgca cgatgcgatt ttttatgatg
     2881 atgttcgggg atattaccgt aaaaccaatc gcagcggagg attggaaggc ggaatgacga
     2941 ccggtgaacg gatcatcgtg cgctgtgcga tgaaaccgat accgacattg gcgcggccgt
     3001 tgatgtcggt tgatatccgt acgcatgaat ccgccagtgc gcataaagaa cgtaccgata
     3061 cggtatcggt gcccgcatgc agtgtcgtgg cggaatccat gttggccttg gctttgatca
     3121 atcccttgct ggaaaagtac ggcggcgaca gtatgaatga tatccgtacg gcgatggggc
     3181 ggtagcttat atcgttaaat tagaaacagt taagagactc ttaagttacg atcatcctta
     3241 gccgatgaac gaaattttga aaaaaattga atattttttc aaaaaacacc gttccatcct
     3301 tgacagctct cgttttgttg cttaactttt atcggtgcac ataatatacc ggccgatcaa
     3361 tcgaattgat tgatttttaa tgatcagaca acacctacac gatttcctca aatatctcga
     3421 cgccgaacgt aatttttccg ataaaacggt gggcgcatac catacggatg tcgcagcatt
     3481 tatttcgttt gttgagacgc gcttcgacag ttcatcattt gatgcatccg atatttccaa
     3541 aaacgaaata cgcgcttatc ttggtcaact cagtcgtgat cggttgcaaa aaaaaagcat
     3601 cgcacgcaaa ttatccgccg taaaatcctt tttcaaatat ctgaccaaac agcaaatcat
     3661 tacggccaat ccggccaaac tcattgctac ccccaaattt gaaagaaaaa tgccggcatt
     3721 ccttggtcag gaacaggtac gcgatgtgtt tgattatgtc gatgccggta cgccggaagg
     3781 cttacgcgat cgcgcaatcc tcgaactgtt ttatggctgc ggtatccgtc ttagcgaatt
     3841 gacgggtctc aatttttccg atatcaattt taccggtggt acgctctcgg ttttcggtaa
     3901 aggcgccaaa cagcggatta ttcctatggg aaaacatgcc ggagcggcat tgcaaagtta
     3961 cattgattac cgacggtccg tttcgtccgc cgataaaaac gccgtatttc tcggaaaaaa
     4021 cggtaaacgg attaccccgc ttgccgttca gcgtttggtc aaacgcgcac tcgaaaaagt
     4081 atccgatgcc aaaaaactaa gcccgcatat tttgcggcat tcgtttgcca cacatttact
     4141 ggacaatggc gcggatctgc gtgccgtgaa agatttgctg ggtcacgaaa atctttcaac
     4201 cacgcaggta tatacgcacg tcacggttga tcgtttgaaa aaagtctatg aaaaagcgca
     4261 tccgcgcgca aaatgaaatg aaaatgtttt gttataacgg gcatgatctt cgattgtgcc
     4321 cgggcttctg tttcccgtcg gttaattatc taatttgaag gagtctgtta tgaaaatccg
     4381 tatcacggca cggcaccaga aaatgagccc taaagtgaaa gcctacatcg aagaaaaact
     4441 ggagaagctg gatcggtatc atgatcatat cattgactgc gaagtcatcg tggacaaaga
     4501 aaaactcaaa gaggtcgtcg aagtcaacgt gaaagttttc ggtaaagtga tcaacgtcaa
     4561 agccaaagat gccgatgtga ccaaagcgac cgatttggcc atggacaaac ttgagtcaca
     4621 gatcaaaaag tttaatgata agatcaaaga caaaaaaaag aagtccgtta aaaaaatgcc
     4681 gatcatcgat gaagatgaag aataattttg aaatccctta ataatccatt acattggggc
     4741 tgttcacaaa aacagcccct tttttataac gtaatctatc aaacataacg catccggcgg
     4801 tgcggcagac gagtactatg gcaaatctca atcaaagtta cgacttcatc aaacaaatcg
     4861 gtgttggcgg catggcgacc gtttttctcg gacggcatcc tgcgttggag cgtctcgtcg
     4921 cgatcaaagt cgtgaagggc gaacaaaaag ataaagtgcg ccgctttgaa cgcgaagccc
     4981 gattgtcggc gacactcaaa caagaaaatc tcccggcgat ctttgattat tttaccgacg
     5041 atcagaataa tcattattta gtgatggagt acgttgacgg cgttgatatc agcccgatca
     5101 tcaaatccaa aacgacgatt cctccgatga tcgtcgccat gatcgctcgc gaagtgtgcc
     5161 gaggcctgga acacatgcac gaaaacggaa ttattcatcg cgatatcaaa cccagcaatg
     5221 ttcgcctcgg aaaaaacggg caagtcaaac tgatggattt cggtattgcc aaacatgaag
     5281 aagacgccgg aaaaagtcac ctgaccgcaa ccggcgtgat cgttggcaca ccgtcatata
     5341 tgtcgccgga acaggcctcg ggcgatcagc tcacaccgca aagtgatgtg tacagtctcg
     5401 gtattatgat gtatgaaatg ctgaccggta aaaagccgta cacggccgat accaacatga
     5461 ccctgatttc gctgatcgcg caggggaaat ttacgccgct atccgagaca catccgcact
     5521 tgccgccggc attggtcgat attgtgcacc gggctatggt caaaagcctc cgacaacgat
     5581 atgcgacgac gagcgagatt atcaaagatc tcaacaaata tcttcaatct atgtcgcaaa
     5641 tagaaattcg tgattggttg gttcgttatt acgaagcggc aaccaccaaa gataaaattg
     5701 tggacatgaa tgtatttgtt ttgccggctc cggtgtctgt ggaaacagcg gaaacctcaa
     5761 cacgcccggt cgatacggaa aaaaacaata attttgttct gaataagcgc cacctcgtct
     5821 tgggcagcgc cgcactggtt gtcttgtgtg ttctggcata tcttttttgg cccagtgatc
     5881 atccgctggg taattatgat ccgtatggcc gtttagcgct gagtgtacgc tccgatcgtc
     5941 agacgatcaa agatacccgt gtctatgtca atgattcgga atttccattg gatgataatt
     6001 ttggcggatc gttgattatt gataatttac aacccggacg caacgcgatt aaaattcgtt
     6061 ttccgctttt gtaccacacg tatgaatata attttacact cgacggtgcg gacgaatcca
     6121 aagctctcaa tttagatctg accaaaatcg cagcttcgtt ggacggtgtt cgtccggagt
     6181 cgcgtcgtta cggatttgcc gttatttctg aaccggccgg cgtatcggtg catcttgatg
     6241 acctcaagtc caaaccgctc ggcaaatctc cgacgggctt gatttttccg tcggtaaaaa
     6301 cgggtttgca taaaatattc ctgcaaaaag aaggttatgt gacgagcgcc atagaacgta
     6361 actttacacc ggatcaaaat tatacattgc aatacgaact gggaccggcc aagaaaaaat
     6421 gatcgaaacg acggacatac cgatcctgcg caaaggcgac gatgtaacgc ttgatatcga
     6481 aacggtcacg tttggcggtc aaggcgtggc gcgttatcat gattttgtcg tgtttgtgga
     6541 gggcgccatt accggcgatc gcgtacaagc ccggataatg aaaaaaaaga aaaattttgc
     6601 cgaagcccga tcgctgcaaa ttatcgaacc ttcacgtttt cgcgtagatc acgcctgcac
     6661 gcattacgga atatgtggcg gatgcaaatg gcaggatgtg gattatgatg cacagttgga
     6721 atttaaacgc cgtaacgtga ttgatgtttt tgaacgcatc ggcggtttta aaggactcga
     6781 tatacccgca ccgctcggat
//
LOCUS       DAOQNQ010000181         6794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_4304_length_6794_cov_5.382698, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000181 DAOQNQ010000000
VERSION     DAOQNQ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4304_length_6794_cov_5.382698"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PK754_10905"
     CDS             complement(<1..322)
                     /locus_tag="PK754_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10058.1"
                     /translation="MYIRSLQIRDFMGFSLYRTEFKEGFSALMMGPSQGKTGFRMAMR
                     VVLTDHFDRNFALFHRNHSGTSEINLELYCGKSALRFNKTWTDGAVQQWTVENPDAGT
                     VLDLT"
     gene            407..1777
                     /gene="der"
                     /locus_tag="PK754_10910"
     CDS             407..1777
                     /gene="der"
                     /locus_tag="PK754_10910"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNB10059.1"
                     /translation="MASKKINRLPVVTIVGRPNVGKSTLFNRIIGRQDAIVDGTPGVT
                     RDRHYEIAEWRGKNFILIDTGGFIPDSHDLINTAIREQAQLAIDESDLVILLIDAVSQ
                     LSTTEMKIADVLKRSKKTSLLIVNKVDNEKVYQNTLADQEIYKLAMGQPLMVSALNSR
                     HIGDMLDRIMTALGDRFAANPDEITTDTNTIRLAIIGRPNVGKSSLVNAIIGKNKHIV
                     SDIPGTTRDAIDTDFIYNDTAFKLIDTAGLRRKAKVKENLEFYSTLRTLKTIQECDVA
                     ILLIDAIEGLAAQDIRVLEEARQLKKGLILVVNKWDLVKKTDKTALDYEKHLDKALGA
                     TKYIPYVFTSALTKQRVAKVLETAKKVHDERNRTIATAQLNRFLERIIARNHPPAVRG
                     KEIKLNYATQIKSRPPVFAIFSNEPTLIPANYRQYIENQMRDEFGFEGVPLSLTFRKK
                     SRDRYE"
     assembly_gap    1792..1891
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1929..3638
                     /locus_tag="PK754_10915"
     CDS             1929..3638
                     /locus_tag="PK754_10915"
                     /inference="COORDINATES: protein motif:HMM:NF039970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6055 domain-containing protein"
                     /protein_id="HNB10060.1"
                     /translation="MINRLFVFIILTTTGYTFAQGRLSQTCKDHADGIISDQQAYDTL
                     HDLLTTSDSTPIHEADKCGFPAIQTLQALSQKLNKSFAVQKKSAAFVYNHAFVTGSGK
                     NRTFKIYYDTVGTDKVSTIDADANTIPDWVEENGKAFEKSYRKEIDTMGYREPVNMKR
                     DGYYEVYIINIRAYGQTAPVQQYPSGSDTWESFIEIENDFVGGGFKTHGYDAMRVTAA
                     HEFHHAIQLAYKYDTGYTDGWFYEMTSTWLEDVVYDEVNDYYAYLPRFYSNPHVSLFS
                     ETTFFGYHVTHWLHMMEKTHGASIVRNIWERMPDERALIAINNALEAETSTDLRNSFT
                     EFSIWNYFTKDRYDSITYFQESRSFPAVSFYKNTLIGNLTYTDGIPGLAARYYNFKTF
                     DSSDIKIRFESGSPSDFNIITIERLESGDPFITRRSHVDSFVVSHMKPGDSLIVIVSN
                     TTTNKTNTTYTISTGLTDGSSSHATVTNLQIYPNPIRMDLHNTLNIKFDLRKTASVVF
                     EVYSISGKRMVHHNYGKFDPNNHLLNWDIKDNAGKFLPSGIYIYRLNIGDYSKTGKIA
                     VVR"
     gene            complement(3653..5083)
                     /gene="pyk"
                     /locus_tag="PK754_10920"
     CDS             complement(3653..5083)
                     /gene="pyk"
                     /locus_tag="PK754_10920"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNB10061.1"
                     /translation="MINLFETRAKIICTIGPSSSDYETLVRMIDAGMDIARLNFSHGS
                     HDAHRQVMDNIRKAAAQVGKQVALLQDLQGPKIRVGIFKNGPIELHIGEKFTITSRDV
                     DGDQHIVSTTYKELPGDVKVGDDLLLDDGLLRLRVVSKTGTDVVCEVIIGGVLKNNKG
                     INLPGVHVSAPSLTEKDAEDLLFGLANDVDYVALSFVRKPEDILHVKEVIRAHGKNTP
                     VVAKIEKPEAIDCIERIIAITDGLMVARGDLGVEMKTEEVPPIQKRLIHLCNRAGVPV
                     ITATQMLDSMVNNPRPTRAEASDVANAILDGSDAVMLSAETASGKYPVETIATMKQII
                     KLIEKEIPHDQSKRRKTGVELQMMQEGIAIAACGLADMLEADAIVNITMTGAMSKLVA
                     KHRPGTPIIAITHSEQVCRQLNLVWGVRGINVADLKEDIDESIEKVKKALVKRRILSA
                     GQRILITAGLPFGGRGATNTVRVETV"
     gene            complement(5098..5631)
                     /locus_tag="PK754_10925"
     CDS             complement(5098..5631)
                     /locus_tag="PK754_10925"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB10062.1"
                     /translation="MKSNRYWFVALVLIGFISSCQSSKTETAADLFKQADSLFTKNKT
                     DEAFKVLAEMETRFKSDTTVMCEVWRVTAEAYAQYKNEFSKTIEYYDRIVNTYPNTPQ
                     AVASLFKKAYTYENGLNDNDRAKAAYEEFIKKYPNHELADDAQTMIDFLGKPADDLIE
                     MLEKKAKQNEKKSTPAK"
     gene            5709..6053
                     /locus_tag="PK754_10930"
     CDS             5709..6053
                     /locus_tag="PK754_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10063.1"
                     /translation="MPSLRATHEIFKTHVLLVLEGRFNPYDSDDDLVVLQNAYDLIRK
                     ETGQTDLILEFDMQHIGSVGINTLIKAYQMARTNDGRLIFIQFPDSEILYLEITGFNR
                     YVSCLQNRTELF"
     gene            complement(6259..>6794)
                     /locus_tag="PK754_10935"
     CDS             complement(6259..>6794)
                     /locus_tag="PK754_10935"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNB10064.1"
                     /translation="GRAVKGSYGKNTSRLKSGSAGEKRAVVYIASAKGNFPAPQSRPA
                     MRQKNIAITPRILPVLAGTTVDFPNEDDLFHNIFSLSPSVKFDLGRYAKGVSKSVKFT
                     TPGVVKVFCDIHSTMGGVVLVLQNPYYTVTEADGSYALSGVPAGTYDLVAWHEGGTQT
                     QKITISASGVITQNFAF"
BASE COUNT         1932 a         1560 c         1345 g         1857 t
ORIGIN      
        1 ttgtaaggtc taaaaccgtg ccggcatccg gattttctac agtccattgt tgtacggcac
       61 cgtcggtcca agtcttatta aatcttaagg cactctttcc gcaatacagc tctaaattga
      121 tttcagacgt gccggaatga ttgcggtgaa acaaggcaaa attgcgatca aaatgatccg
      181 tgagtacaac gcgcatggcc attcgaaaac cggtttttcc ttgggacggc cccatcatca
      241 aagcagaaaa cccctctttg aattcggtcc gatacaacga aaaacccata aaatcgcgta
      301 tttgaagcga tcgtatatac ataaattcct atttaaacct tgaaatagta cgcgctgttg
      361 gttaggttat taaatcaagt atatatattc aatattgact tttaaagtgg cctcaaaaaa
      421 aatcaaccgc cttcccgtcg tcacgatagt cggacggccc aatgtaggaa aatcaacgct
      481 attcaaccgc atcatcggac gtcaggatgc catcgtagac ggtacacccg gagtaacacg
      541 ggaccgtcac tatgaaatcg ccgaatggcg tggaaaaaat tttattctca tcgatacggg
      601 cgggtttatt ccggattcgc atgatctcat caataccgct atccgtgagc aagcgcagct
      661 ggctattgac gaatccgatc tcgttatttt actgattgat gccgtaagcc aactttccac
      721 gacagaaatg aaaattgccg atgttttaaa acgttctaaa aagacatcgt tattgatagt
      781 caataaagtg gacaacgaaa aagtttatca aaatacactg gcggatcagg aaatttataa
      841 actggccatg gggcaaccgc tgatggtatc agcgctgaac agtcgccata tcggcgacat
      901 gctcgaccgt atcatgacgg cgcttggtga ccgttttgcg gccaacccgg acgaaataac
      961 gaccgatact aacactattc gtttggctat tatcggacgc cccaatgtcg gaaaatcttc
     1021 tttggtcaac gccatcatcg gtaaaaataa acatatcgtt tcggatatac cgggaacaac
     1081 acgcgacgcg attgatactg attttattta caatgatacc gcattcaaat tgattgatac
     1141 ggccggtctg cgacgcaaag ccaaagtaaa agaaaatctc gaattttaca gcacccttcg
     1201 tacactcaaa acaatacaag agtgcgatgt agcgattctc ttgattgatg cgattgaggg
     1261 gctcgcggct caggacattc gcgtactcga agaagcccgg caactgaaaa aaggattaat
     1321 tttagttgta aataaatggg atcttgttaa aaaaacagac aaaacggcgc ttgattacga
     1381 aaagcatttg gacaaagcgc tcggcgcaac caaatatatt ccgtatgttt ttacatcggc
     1441 gcttaccaaa cagcgcgttg ccaaagtcct cgaaaccgct aaaaaagtac atgacgagcg
     1501 taaccggact attgcaacag cgcaactcaa ccgctttctc gaacgcatca ttgcgcgcaa
     1561 tcacccaccc gccgtacgcg gtaaagaaat caaactgaac tacgcgacgc aaatcaaatc
     1621 acggccaccg gttttcgcga ttttctccaa tgaaccaacc ctcattccgg ctaactatcg
     1681 ccagtacatc gaaaatcaaa tgcgggatga gtttggtttt gaaggcgtcc ctttgtcact
     1741 aacttttaga aaaaaaagca gggatcgtta cgaatgataa accgtctatt tnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncactaactt ttagaaaaaa aagcagggat
     1921 cgttacgaat gataaaccgt ctatttgtat ttataatcct tactacaacc ggttatacct
     1981 ttgcccaagg acgactttcg caaacgtgta aagaccacgc cgatggcatt atcagcgatc
     2041 aacaagccta cgatacactg catgatttgc ttacgacttc ggattctaca ccgatccatg
     2101 aggccgacaa atgcggtttt ccggcgattc aaactttaca agccctttct caaaaattaa
     2161 ataaatcgtt cgctgtgcag aaaaaatctg ccgcatttgt ttataatcac gcgtttgtaa
     2221 ccggttcagg aaaaaatcgt acgtttaaaa tatattacga tacggtcggc acagataaag
     2281 tatcaaccat tgatgccgac gccaatacca ttcccgactg ggttgaagaa aacggaaagg
     2341 cttttgaaaa atcataccgt aaagaaattg ataccatggg atatcgtgaa cccgtcaata
     2401 tgaaacgtga cggatattat gaggtataca ttatcaatat cagagcttac ggtcagacgg
     2461 caccggtgca gcaatatcca tccggaagcg atacatggga atccttcatt gaaattgaaa
     2521 atgattttgt aggcggtgga ttcaaaacgc acggttatga cgccatgcgg gtaacggcgg
     2581 cgcacgaatt tcaccacgcc attcaattgg cctataaata cgataccggt tatacggacg
     2641 gttggtttta tgaaatgact tcaacttggc tggaagatgt agtatatgac gaggtcaacg
     2701 actattacgc ttatctacca agattttaca gcaaccccca cgtgtcttta ttcagcgaga
     2761 cgacgttttt tggttaccat gttacacatt ggttgcatat gatggaaaag acccacggcg
     2821 cttctatagt tcgaaatata tgggaacgca tgcccgacga acgggcgttg atcgctataa
     2881 ataatgctct cgaagccgaa acaagtactg accttagaaa ttcatttacc gaattttcaa
     2941 tctggaacta ttttacaaaa gatcgttatg acagtattac atattttcag gaaagtcgct
     3001 cgtttcctgc ggtaagtttt tataaaaata ctttgatcgg taatttaact tataccgatg
     3061 gcatacccgg tttggcggca cggtattata actttaaaac ctttgactcc agtgatatta
     3121 aaattcgttt cgaatcaggt agcccttccg attttaatat tattaccatt gaacgtttgg
     3181 aatccggcga tccttttata acccgtcgga gccatgttga cagttttgtt gtatcgcata
     3241 tgaaaccggg tgattctttg atcgttatcg tcagcaacac gactacaaat aagaccaata
     3301 cgacctatac gatcagtacc ggtttaacag atggctcttc ttcacatgca acggttacta
     3361 atctgcaaat ctaccccaat ccgatccgaa tggaccttca caacacgctc aacatcaaat
     3421 ttgatctgcg aaaaacggca tctgtagttt tcgaggttta ttcaatttcc ggaaaacgga
     3481 tggtacatca taactacggc aaatttgatc ctaataatca tcttttaaat tgggatataa
     3541 aagataatgc cggaaagttt ttaccctccg gcatttatat ctatcgtttg aatattggcg
     3601 attattccaa aaccggtaaa atcgctgtcg tgcgttgatg cggttttaca gactaaaccg
     3661 tttcaacccg aaccgtattg gtcgcaccgc gtccgccaaa aggcaaacct gcggttatta
     3721 ggatacgctg gcctgcagat agaatcctac gttttacaag agctttttta actttttcaa
     3781 tactttcatc aatatcctct ttcagatcag caacattgat accgcgtacg ccccacacaa
     3841 gattaagttg acgacatacc tgctccgaat gtgtaattgc aatgatgggt gtaccgggac
     3901 ggtgtttagc cacgagctta gacatagcgc ctgtcatcgt aatattaacg atggcatcgg
     3961 cttctagcat atcggcaagc ccgcaagcgg cgatggctat cccctcctgc atcatttgca
     4021 attctacgcc tgtcttgcga cgtttgctct gatcgtgggg aatttctttt tcgattagtt
     4081 taatgatttg tttcatggtg gcgatggttt ccaccggata tttacccgat gctgtttccg
     4141 ccgataacat cacggcgtcc gaaccgtcca atatggcatt ggcaacgtcc gaagcctccg
     4201 cacgcgtcgg acgcggattg ttaaccatcg aatcaagcat ttgtgttgcc gtgatcaccg
     4261 gcactccggc acggttgcaa agatgaatca atcgcttctg aatgggcgga acttcttcgg
     4321 ttttcatctc aacgccaaga tcaccgcgtg caaccatcaa tccgtccgtt atcgctataa
     4381 tgcgttctat acaatcaata gcttccggtt tttcgatttt tgccacgacc ggtgtattct
     4441 tgccgtgagc gcgtatcact tctttaacat gcagtatatc ttccggtttg cgaacaaacg
     4501 aaagggcgac ataatctaca tcattagcca aaccgaaaag tagatcctcc gcatcttttt
     4561 ccgtcaacga aggtgcactg acatgtacac cgggcaaatt aattcctttg ttatttttca
     4621 acacgccgcc gatgatgact tcacacacca catcggtacc ggttttagaa acaacacgaa
     4681 gcctgagtaa tccgtcatct aaaagtaaat catcaccgac ttttacgtcc ccgggcagct
     4741 ccttgtacgt ggtggaaacg atatgctgat caccatctac atccctggat gtaattgtaa
     4801 atttttcacc gatgtgcaat tcaatggggc catttttaaa aattcccaca cgtattttag
     4861 gaccctgtaa atcctgaaga agcgctacct gcttaccaac ctgcgccgcc gctttacgaa
     4921 tattgtccat tacctggcga tgcgcgtcat gtgagccgtg cgaaaaatta agacgcgcaa
     4981 tatccatacc ggcatcaatc atccttacca aagtttcgta atcgctcgat gacgggccaa
     5041 tcgtacaaat aattttagcg cgggtttcaa ataaatttat catagactcc gattaagtta
     5101 tttagcaggt gttgattttt tttcattttg tttagccttt ttctccagca tttcgatcaa
     5161 atcatccgcc ggtttaccga gaaaatcgat catcgtttgc gcatcatcgg ctagttcatg
     5221 attgggatat tttttgataa attcttcata cgccgctttt gcgcgatcat tatcattcaa
     5281 cccgttttca taggtgtatg cttttttgaa taaactggcc acggcttggg gcgtattggg
     5341 ataggtattc acaatacggt cataatattc tatggttttg ctaaattcat ttttatactg
     5401 cgcatacgct tcagccgtta cacgccacac ttcacacatc acagtcgtat cggatttaaa
     5461 acgcgtttcc atctcggcca acactttaaa cgcttcatcc gttttgtttt ttgtaaaaag
     5521 gctgtccgcc tgtttaaata aatcagccgc cgtctccgtt ttcgacgatt ggcatgatga
     5581 gataaaacca atcaaaacca acgcaacgaa ccaataccta tttgacttca tatcggctta
     5641 atccttttat attgtttgaa aataagtttg tgtgtagtat ctctgttgaa attacaaata
     5701 aaccctatat gccatcccta cgcgcgacac acgaaatttt taaaacccat gtgcttctag
     5761 tcctcgaagg ccgattcaat ccgtacgatt cagacgatga tttggtcgtt ctccaaaatg
     5821 cctacgatct gatccgcaaa gaaaccggcc aaacggattt gatccttgaa tttgacatgc
     5881 aacacatcgg ttcagtcgga atcaatacgt tgatcaaggc ttatcaaatg gctcggacga
     5941 atgatggacg gttaatcttc attcaatttc ctgattcaga aatcctctat cttgaaatca
     6001 caggctttaa tcgctatgtg tcgtgcctgc aaaaccgcac ggaacttttc tgaaaaagcg
     6061 gcctaatgta cacactcacc cgatttcttg caagcgataa acccatatcg gttcgacgga
     6121 tcatcacgga gtttaatgaa ctgaaaacaa gatatgataa agcaaaaaat aaatgccttt
     6181 atggcctatc aggcttgggt ataaatagaa aaacctgccg aaagcaggtt ttttttcact
     6241 taacaatggt atgacttctc aaaaagcaaa attttgtgtg atcactccgg aagctgatat
     6301 cgtaattttt tgagtttgcg tacccccttc atgccatgcc accagatcat acgtccccgc
     6361 cggtacaccg gataatgcat aacttccatc ggcttccgtc acggtataat acgggttttg
     6421 taaaaccaga acgactccgc ccatagtcga atggatgtca caaaacactt tgacaacacc
     6481 cggtgtagta aatttgacag acttggatac acctttggca taacgcccga gatcaaattt
     6541 gacggaaggc gaaagtgaaa aaatattgtg aaacaaatca tcttcattgg ggaagtccac
     6601 tgtcgttccc gccagcaccg gtaaaatacg tggtgtaatg gctatatttt tttgacgcat
     6661 agcaggtcgg ctttgcggcg ccgggaaatt acccttggcc gaggcaatat ataccaccgc
     6721 tcttttttcg ccggcggaac cggattttaa acgcgatgtg ttttttccgt aggagccttt
     6781 tacggcacgc ccgg
//
LOCUS       DAOQNQ010000182         6785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_4311_length_6785_cov_5.251263, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000182 DAOQNQ010000000
VERSION     DAOQNQ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4311_length_6785_cov_5.251263"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PK754_10940"
     CDS             complement(<1..273)
                     /locus_tag="PK754_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10065.1"
                     /translation="MRILVMLVSLIVGASLVAQQRTTIKFGTGKIDMTCSNKAAAGFY
                     KNIEIRSTDTPIRIYFMKNDKTVMDVSGSAVYISYEQDTRGDDVAAV"
     gene            complement(348..1205)
                     /locus_tag="PK754_10945"
     CDS             complement(348..1205)
                     /locus_tag="PK754_10945"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019243242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HNB10066.1"
                     /translation="MEIKRIAVIGAGTMGNGIAHVFAQTGYDVNLIDIKQEFVERGKA
                     NIEKNMGRQVTKGTLTEEQKNAALVRITLCTKIEDAANCDFVVEAASENPEIKAGIFK
                     TLDNVCKPDVILSTNTSSISITQIAAVTKRADKVIGMHFMNPVPVMKLIELIRGLATS
                     QTTYDTVKTMSEKLGKVPVEVNDYPGFISNRVLMPMINEAIFCLMEGVASVEAIDTVM
                     KLGMNHPMGPLQLADFIGLDVCLSIMNVLHEGLGDDKYRPCPLLKKMVTAGYYGVKSG
                     KGFYDYPKK"
     gene            complement(1374..1745)
                     /locus_tag="PK754_10950"
     CDS             complement(1374..1745)
                     /locus_tag="PK754_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB10067.1"
                     /translation="MAKICVIEDEPNLRLLYRSEFERMGHEVICHANGREAYRSLPVD
                     QPDLIILDIMMPEGDGLEFLSNVLVMHVGIPVIINSAYAQFKNDFVSWAAEAYVVKSS
                     DLSELKEQVHRVLFKFSMATA"
     gene            complement(1860..2654)
                     /locus_tag="PK754_10955"
     CDS             complement(1860..2654)
                     /locus_tag="PK754_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HNB10068.1"
                     /translation="MEINCLHIADIFAQSGLDMVTETLPKLIKERDIHFVVANGENTL
                     EGKGITEGQCQHLFNLGVHVITSGNHIWDRKKTFYAENPLIQKYLLRPLNYPVGNEGR
                     GSTVYELKNGLKIGVINLQGRTYMYDIDCPFRVGEREIEKLQDETNIIIVDFHAEATA
                     EKIAFRHYVDGRVSAVIGTHTHVQTADEMITSSGTAYITDAGMTGPHDGVIGMQKEPT
                     VKKFILQTYFKFEPSKGDVRFHGLFFKVNAETGRATYVERVRIDKS"
     gene            complement(2692..4524)
                     /locus_tag="PK754_10960"
     CDS             complement(2692..4524)
                     /locus_tag="PK754_10960"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HNB10069.1"
                     /translation="MMYKNLSDCIADLERNGHLIRVNEEVDPYLEMAEIQRRVYQKQG
                     PALLFTRVKGCRFPAVSNLFGTMDRTRFMFRHTLAAVQKLIELKVDPANLAKRPLRYA
                     GVPFSALTMLPWKKSNGAVLKNTCRIGELPQITCWPKDGGPFVTLPQVYSEHPDHPGW
                     KMSNLGMYRIQLAGNQYIQDREVGGHYQIHRGIGVHHAAAIRRGEKLRVSIFVGGPPA
                     HTLAAVMPLPEGLSELMFAGVLTGRRFRYGYQNGHAVSMDADFVITGTIDTQATKPEG
                     PFGDHLGYYSLTHDYPYIEVEQVYHRDGAVWPFTVVGRPPQEDTSFGDIIHEITGPVI
                     PTVIAGLHGVNAVDGAGVHPLLLAIGSERYVPYGERKPQELLTIANAILGQGQLSLAK
                     YLFITAKEDDPSLDVHDIPKFLQHVLSRVRWEEDLHFQTRTTIDTLDYSGSGFNQGSK
                     VVIASAGKVRRLLASQVPSELKLPEGFSDARVILPGIVAIRGTQYSDRVAARKAIRVL
                     CEYLEHQRDFENLPLWIVCDDVTFVKAQLNNFLWVTFTRSDPAADMDGAGAFVEDKHW
                     GCRGPLVIDARIKPHHAPPLIEDPDVTRRVDALAEKGKSLYGII"
     gene            4784..6172
                     /locus_tag="PK754_10965"
     CDS             4784..6172
                     /locus_tag="PK754_10965"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNB10070.1"
                     /translation="MQHSIVWLRRDLRLDDNTALLHAMKASQFVSLIFIFDKHILDRL
                     PADDRRLSFIYESLTYLQNKLKAHGAPTVHIFYGEPEVVIPQLFSENKTDALFFNHDY
                     EPYANVRDTAITEVLNQKGILVHSFKDQVIFEKSEIVKDDGTPYKVFTAYKNRWLNTY
                     THSYPNAITDSTSIPWKKFSPLFSSLPLIHSLQEIGFVQCENIIHGGSDAAQKQWTQF
                     TGDYIEKYDTQRDLPGLNATSHLAPHLRFGTISVRQLIDYLSKHEAPAVKVFLSELIW
                     REFFMMILWHFPHVIIRSFQPQFDRIVWSNREDWFEAWKSGHTGYPIVDAGMRELHAT
                     GYMHNRVRMIVASFLVKHLHIDWRKGEAYFAEKLLDYELSSNNGNWQWAAGTGVDAAP
                     YFRIFNPLTQGKKFDPDAVYIRRWVPELENVSLREIHDPIKRQSGLFSHYPHPIIDLD
                     QERQKCLALYKV"
     gene            complement(6230..6586)
                     /locus_tag="PK754_10970"
     CDS             complement(6230..6586)
                     /locus_tag="PK754_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10071.1"
                     /translation="MLSLLAFVGCSESEDIGNVPGPPDPPKPYSYVTDIKAVLDEGCA
                     SCHSGGSPSGSYNLSTYAGVLGNGSDATPNAIPGDSTSLIILKLKSGHQTPDASTISM
                     IFQWVYQDSCKETLPD"
BASE COUNT         1874 a         1615 c         1439 g         1857 t
ORIGIN      
        1 aacagcggct acatcatcgc cccgtgtatc ttgttcatac gaaatgtaaa ccgctgaacc
       61 ggatacgtcc ataacggttt tatcgttttt catgaaataa attcgaatcg gcgtatcggt
      121 tgaacgaatt tcaatgtttt tataaaatcc tgccgctgct ttattggagc aggtcatatc
      181 aattttcccg gttccaaact tgatggtggt tcgctgttgc gcaacgagcg acgcgccaac
      241 gatcaggcta actagcatta ctaaaattcg catagacagt cctttatatt gttatacaac
      301 gacgtaagtt acattgtcgc gtgtacgaat tcaaccgaaa tttacgatta ctttttgggg
      361 tagtcgtaaa aaccttttcc gctttttacg ccataatacc ctgctgttac catttttttg
      421 agtagcggac acgggcggta tttgtcgtca ccaagacctt catgcaatac attcatgatg
      481 gaaagacata cgtccaaacc gataaaatcg gcaagttgca gcggccccat aggatgattc
      541 atgccgagtt tcattacggt atcaatggct tctacggatg ccacaccttc cataagacag
      601 aatatcgctt cattgatcat aggcatcaac acgcgattgg aaataaaacc cggataatca
      661 ttgacctcga caggcacttt gccaagtttt tcggacatag ttttgaccgt gtcatacgta
      721 gtctggcttg ttgctaatcc acggatcaat tcgatcagtt tcatgacggg cacgggattc
      781 ataaaatgca tcccgatcac tttatcggca cgtttggtca ctgcagcgat ctgtgtgatc
      841 gaaatcgaag acgtattggt ggataaaatc acgtcaggtt tacatacgtt atccagagtt
      901 ttaaaaatgc ccgcttttat ttccgggttt tcggatgccg cttcgacaac gaaatcacag
      961 ttagccgcat cttcgatttt ggtgcaaagc gtgatccgca ccagtgcagc gtttttttgt
     1021 tcttccgtca aagtaccttt ggtaacctga cggcccatgt ttttttcgat attcgcttta
     1081 ccgcgttcga caaattcctg tttgatatcg atcaaattga catcgtatcc ggtttgcgca
     1141 aaaacgtgag caattccgtt tcccatagta ccggcgccga tcacggcgat acgtttgatt
     1201 tccacagatt catctcctgt tattatgtat aatctcgtga tatgttaagt gcggctaaat
     1261 gtacccaaac cttaagagaa gctcaataaa aaatcccgct gtgacgacag cgggatgagt
     1321 aagactcacg cgccttatga taaggcgtga attaaggcgt gcccatctat gggtcacgcg
     1381 gtggccattg aaaacttgaa gagaacgcga tggacttgct ctttcagttc acttaaatcg
     1441 ctggacttga cgacatacgc ctcagccgcc caactgacga aatcattttt aaactgcgcg
     1501 tacgctgaat tgataatcac cggtatgcca acatgcataa ccagtacatt tgagagaaac
     1561 tccaaaccat cgccttcagg catcatgata tcaaggatga tcagatcggg ctgatcaacc
     1621 ggaagtgatc ggtaagcttc gcgcccattg gcgtggcaaa tgacttcgtg ccccattctt
     1681 tcaaactcag aacgatacaa tagccttaga ttcggctcat cttctatgac acaaatctta
     1741 gccatcgcgc tctcctttgg gaaaaaagtt cctttcaaga acctatcaat taaacttcac
     1801 ggctcccaaa aaagttccat ccgcggatgc aatgctgtta aagcttgata agaaatgagt
     1861 tatgatttgt ctatgcgcac acgttcgaca taggttgcac gcccggtttc ggcattaacc
     1921 ttaaaaaaca agccgtgaaa gcgcacatcc cctttggaag gctcaaattt gaagtacgtt
     1981 tggagtataa atttttttac cgtgggctct ttttgcatac cgatcacacc gtcgtgcggg
     2041 cccgtcatac cggcatcggt aatatacgcc gtaccggatg aggtgatcat ttcatccgcc
     2101 gtttgtacat gcgtatgtgt cccgatcacg gcactcacac gcccatcaac ataatgacga
     2161 aacgcaattt tttcagcggt cgcttccgca tgaaaatcta caataatgat attggtttca
     2221 tcctgaagtt tttcaatttc gcgttcaccg acgcgaaaag ggcaatctat gtcatacata
     2281 taggttcgcc cttgcagatt gatcacaccg attttgagac cattttttaa ttcgtatacc
     2341 gtcgagccac gaccttcatt gcccacggga taattgagcg ggcgaagtaa gtatttttga
     2401 atcaacgggt tttccgcata aaatgttttc tttcgatccc aaatatggtt accgctggtg
     2461 atcacatgta cgccgagatt aaaaagatgt tggcattgcc cttcggttat acctttacct
     2521 tcgagcgtat tttcaccgtt tgccacaaca aaatgaatgt cacgttcctt gatgagtttg
     2581 ggcagcgttt ctgtgaccat atccaaaccg gattgtgcaa aaatatcggc aatgtgcagg
     2641 caattgattt ccatgatgct ccaaaagtta accgttcaat gaatccgaaa cttaaataat
     2701 accgtacaaa ctttttcctt tttcagccaa tgcatccaca cgacgcgtga catcgggatc
     2761 ttcgataagc ggcggtgcgt gatgcggttt gatccgtgcg tcaataacca gaggtcccct
     2821 gcaaccccaa tgtttatcct ccacaaaagc acccgctccg tccatatcag ccgccggatc
     2881 ggaacgtgta aaagtcaccc acagaaaatt gttgagttgt gctttgacaa acgtcacatc
     2941 atcacaaact atccataatg gcaagttttc aaaatccctc tgatgttcca aatattcgca
     3001 taaaacacga atggcttttc gagcagctac gcggtcggaa tattgggttc ccctgatcgc
     3061 aacaataccc ggtaaaataa cgcgcgcatc ggaaaaacct tccggtaatt ttaattccga
     3121 aggcacctga cttgccagta agcgacggac ttttcccgcg gatgctatca ccactttaga
     3181 cccttgatta aagccggaac ctgaataatc caatgtatca atcgtcgttc gtgtctgaaa
     3241 atgcagatcc tcctcccaac gcacccgtga aagtacgtgt tgcaaaaatt taggaatatc
     3301 atgcacatca agcgaaggat cgtcttcttt tgcggtgatg aagagatact ttgccaaaga
     3361 aagttgcccc tgaccgagaa tggcattggc gatagtaaga agttcctgcg gtttacgttc
     3421 accgtaaggc acataacgct cgctgccgat cgcaagtaac agcggatgaa cacccgcgcc
     3481 gtccaccgca ttgacgccat gcaaaccggc gatcaccgta ggtataaccg gccctgtgat
     3541 ttcatgaata atatccccga acgatgtatc ctcttgcggc gggcgtccga caacggtaaa
     3601 cggccataca gcgccatccc ggtgatacac ctgctccacc tcaatgtatg gatagtcgtg
     3661 cgtgagactg tagtaaccta agtgatctcc aaaaggacct tccggttttg tcgcttgcgt
     3721 atcaatagta cccgtaatca caaaatcagc atccatggat accgcatgac cgttttggta
     3781 accataacga aaacgccgtc ctgtcagtac acccgcaaac atcagttcgg ataaaccttc
     3841 gggtaagggc attaccgccg ccaaggtgtg cgcaggcgga ccgccgacaa aaatgctaac
     3901 acgcaacttt tcaccacgac gaatggcggc cgcatggtgc acgccgatac cgcgatgaat
     3961 ctgataatgg ccgccgacct cacgatcctg gatatactga ttaccggcca gttgtatgcg
     4021 gtacatgcca agattggaca ttttccatcc ggggtgatcc ggatgttcgg agtaaacctg
     4081 cggcaatgtg acaaacggcc cgccatcctt gggccaacaa gtgatttgcg gaagctcacc
     4141 gatacggcat gtatttttta aaaccgcacc gttgcttttt ttccatggca acatcgtcaa
     4201 cgctgaaaat ggaactccgg cataacgcaa cgggcgtttg gccagattcg ccggatcaac
     4261 ttttaattcg atcagttttt gcacagccgc gagcgtatgg cgaaacataa agcgcgttcg
     4321 gtccatcgtt ccaaaaagat tggaaacagc cgggaaacga caccctttga cgcgggtaaa
     4381 taataatgcc ggaccttgtt tttgatatac gcgtcgctgg atctccgcca tctcaagata
     4441 gggatccact tcttcattaa cgcgtatcaa atgaccgttc cgttccaaat cggcaataca
     4501 gtcggataaa tttttgtaca tcatagcgtg gtagtgtaag caataaaaaa cacttctaaa
     4561 agtgttttga aaataagaaa tattgtttaa tatatgcgat gattttttaa acaattatat
     4621 ttttatataa tacatgcaca aaaggtcatc catacacagc tcgtagcaaa atcacagacc
     4681 gtttttgatg tattgcaacg gttggttcgt cagtttcggt ttgcacgata acttgtacag
     4741 caagtgcaag aacatagtta aattcaaaac aaaagataaa tacatgcagc acagcattgt
     4801 ttggctaagg cgggatctac gattagacga taatacggcg cttctccacg ctatgaaggc
     4861 aagccaattt gtttcactta tttttatatt tgacaaacat attttggatc gtttgccggc
     4921 cgatgaccga cgattgagtt ttatttacga atcgctcacc tatcttcaaa acaaattgaa
     4981 agcccatggt gcgccgaccg tacatatatt ttacggtgaa ccagaagtcg tcattccgca
     5041 gctattttct gaaaacaaaa ccgatgcatt gttctttaat cacgactatg agccgtatgc
     5101 caacgtgcgc gatactgcga taacggaggt attaaaccaa aaaggaatcc ttgtacactc
     5161 ttttaaagat caggtcattt ttgaaaaatc agaaatcgta aaagatgacg gcacgcctta
     5221 taaagtcttt accgcttata aaaaccgatg gctgaatacg tatacccatt cttatccgaa
     5281 tgcaataaca gatagtacat ccataccttg gaaaaaattt tctcctctgt tttcgtcgct
     5341 accgcttatt cattcgctgc aagagatcgg gtttgttcag tgtgaaaata tcatacacgg
     5401 cggttccgat gccgctcaaa aacaatggac acaatttacc ggtgattata tcgaaaaata
     5461 cgatacacaa cgcgacttgc ccggactgaa cgctacctct caccttgcgc cacaccttcg
     5521 ttttggtacg atcagtgttc gccagcttat agactatcta tcaaaacatg aggctccggc
     5581 ggttaaagtt tttttgtcgg aactcatctg gcgtgaattt tttatgatga tactttggca
     5641 tttccctcac gtcataatcc gtagttttca accccagttt gatcgcatcg tatggtctaa
     5701 tcgcgaagat tggtttgaag catggaaatc agggcatacg ggttatccca tcgtggatgc
     5761 cggcatgcgt gaactccatg caacgggata tatgcacaat cgggtacgga tgatcgtggc
     5821 ttcatttttg gtcaaacatc tgcatataga ttggcgcaaa ggcgaggcgt attttgcaga
     5881 aaaactgttg gactatgaat tatccagcaa taacggtaac tggcaatggg cagccggtac
     5941 cggtgtggat gctgcgccgt attttcggat ttttaatcct ctgacccagg ggaaaaaatt
     6001 tgacccggat gccgtatata ttcggcgatg ggtaccggag ctggaaaatg tttcgcttcg
     6061 tgaaatacat gacccgatta aacggcaaag cgggttattc agccattatc cgcatcctat
     6121 cattgatctg gatcaggaac gacagaagtg tttagcactg tacaaagttt aaaaaaatcc
     6181 ccgaacgaaa aatcgtccgg ggattctact ctatcaaaaa aacagtttgt tagtccggca
     6241 gtgtctcttt gcagctatcc tgataaaccc actgaaatat catagaaata gtagatgcat
     6301 cgggagtttg atgaccggac tttagcttca aaataatgag gctggtcgaa tctccgggta
     6361 ttgcattcgg cgttgcatcg gaaccatttc ctaaaactcc ggcataggtc gatagattat
     6421 aactgccaga tggactacca cccgagtggc atgatgcaca tccctcatca agtacagctt
     6481 ttatgtcggt tacatagctg tatggttttg gcggatccgg tgggccgggt acattaccaa
     6541 tatcttcgga ttcgctgcag cccacgaagg ccaaaaggga taacattatt attaatattt
     6601 tctttttcat ttggctgtcc tcagaaattt ttaagtgaac catcggcttt atgacacgtg
     6661 taacaagtcg gatcctgccc ttcgatgtta tggcaactta cacagttttc catgtctttt
     6721 ttggctaact gagcatgcat tccaccacca gcacctctgt caacaaaacc tgcaactttg
     6781 tgcca
//
LOCUS       DAOQNQ010000183         6738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_4344_length_6738_cov_5.816400, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000183 DAOQNQ010000000
VERSION     DAOQNQ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6738
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4344_length_6738_cov_5.816400"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="PK754_10975"
     CDS             complement(<1..1038)
                     /locus_tag="PK754_10975"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB10072.1"
                     /translation="MESIGVNSNVYAGGKVFHIQTAGNLSSLLVKAEIFDSGRIVTVH
                     QKKLDPEQYKYLDTDRFRDVVAAFHQDIAWEIELLFFIRDRIKTIKHAVSSNKIGLLF
                     LHKGLVDEAIAEFENAITINPQMVESYRNLGLAFVEKKETQKAIEVLHKAIELEPRYA
                     DLHNSLGLAYALNGELDTALQTYETALAINPNYLEVHLNIGMVILRMHAERFQSAVFA
                     PSSIKPTRVINELQKLMNARTEFINYRFFSFRFEKAIRQIQDNDFLAAILTLKDIREA
                     TQTPNLDETMHGFYLKFLFGGAGKDEKVLGEYRARLEKSVEEHPHFADLHNSLGVVYL
                     IQCRNLFIKAMS"
     gene            complement(1187..3328)
                     /locus_tag="PK754_10980"
     CDS             complement(1187..3328)
                     /locus_tag="PK754_10980"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNB10073.1"
                     /translation="MTEQVRNRLFMAFVALFPIPFIVWCFFLMRSMTENQTQIQSHTD
                     KIARIYKLQQAVYGLEEVISDQFLAATDTSFDDWNRIYQKAWQASLGVDWTDGSVFDM
                     RNALPRIDNLYQRIHKRKFKRTSIIPDSIRVENLTGEMIDEIHRAAESLGISAEKLRT
                     RSIRYQESLSQDWHTAIKFLIIASAITILISVIAVFYRYSLQRNKKLALLLYRLNENV
                     SGILGNTPDGVCTVDEHFKVLLINEPFKRLFHLRHQIELHPGSNMIDHLKPDAQAAWV
                     IVLTRAMRGEKFMIEQQFQLPDRLVLAEVSFNPVLVNNKVNGTAIHIHEVTKQKRSQE
                     TADRKNKELQDTIERQFRMIETIVQTAHDGAMIVDAAGQFPFQNDVARDIMTTAGTPT
                     SLSLWSGIHGVFMEGSEQPIDNQKLLERARSGEVFTILVRNERYMEGQKFEVSASSLT
                     EGDDLSRIIAFKAPPEIIPVMQEVPVPEIALKGALEEIPQWAFTINREMAIQSVNTAF
                     EKRFNKPRYTTIGYDIRQLIHPDDQPAFESRIQEALPEHPVHTAFRFLIDGQATHMET
                     RIIGGALTILCVGYSIEDMLLAEAAEDMRSAPQPLFTFDCDAYGVITQIEGDRTLMLP
                     SYRSEWQGQSMFKLFEQDLPMQEAFYRALDGQPIQRNAAYGQQEVTIVYQPVFDGVGI
                     IAGLKIQIFDAAALLAFQEKIKTRLSEKASI"
     gene            3476..3946
                     /gene="tadA"
                     /locus_tag="PK754_10985"
     CDS             3476..3946
                     /gene="tadA"
                     /locus_tag="PK754_10985"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HNB10074.1"
                     /translation="MDTHETWMAMAFKEAEKAYAKNEIPVGAVIVLENRIIGRGHNQV
                     EMLQDPTAHAEMIAMTSAVATIGSKWLYGATMYVTLEPCSMCAGALVLSRMSQLVFGA
                     GDPKTGACGSLHNIVQDERLNHRIEVLRGVMDAKCSAILKDFFAKLRKPAPQDN"
     gene            4093..5955
                     /locus_tag="PK754_10990"
     CDS             4093..5955
                     /locus_tag="PK754_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10075.1"
                     /translation="MTIRSVLLWCCFVLCLPAVQAQKVKLKTGSVKEKKDEKKENKTE
                     TTSSGTFKVGDVAEGLETDGKWYKVDILKVEADKYFIHWQGYKSTYDRWIEAGKVRAI
                     GSSGSASANASTSAASTASGGKTKVYGTGDGGFKVGEYAEVLDRDGKWYEAEIQKVNE
                     GQPNEYFVKIENYNGFWVKESNVRAKSSGTSSEPVGFKVGDVIEAVDFGRWNKGTVQS
                     IRTTNSGNKMFTVAFDNGGSKEFTENKLRVIPVYNFSGSNYKTGQYIRIWYSGRDSLD
                     APIVDIKDNKLLINYGGYGAKWFDFDAKEIRNDARTAAIAASKATEEQFFQDCNKYSE
                     AIRIFGYLYNKKHDTRFVLYNQGLTSPRPSDVGPMLKQMNELDALIKSKYPNIQNTEQ
                     TWTTELSKNPGHWRVIAEERNELAQSVVTDMIKDKISEATRILTNYEFLKNHEIGEFE
                     AGKGIGVTSSLQDILDNNGADFMKKEEEKIADVIKAAQEANLGQNEITAFREFLKKLS
                     VDVKSKFPSSLVSSQFNPALHDAAAEASAKAYIQKTYSGAAILKIGVIGNQVIEKNNL
                     GIPLYRYKRVGVLFRDSQGRCRFVPAYYREDYAGGGAYGTPYIEHHFTYYQSCN"
     gene            complement(6052..>6738)
                     /locus_tag="PK754_10995"
     CDS             complement(6052..>6738)
                     /locus_tag="PK754_10995"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNB10076.1"
                     /translation="IQTLKVGSLGTHKALGLAKSKDAAFLLASTSETYGDPLINPQHE
                     EYWGNVNPVGPRGVYDEAKRFAEAMTMAYHTFHNVDTRIVRIFNTYGPRNRPNDGRVV
                     PTFINQAIRNQPITVFGEGKQTRSFCYCSDLIDGIYRLSQSSEHYPVNIGNPHEMTVL
                     QFAQLIIRLTNSKSEIVYRPLPKDDPTVRRPDISKAKRLLNWEPTVDIETGLLKTIEY
                     FSEKMKREGK"
BASE COUNT         1819 a         1471 c         1695 g         1753 t
ORIGIN      
        1 gctcatggcc ttaataaaaa ggttgcggca ctggatcaaa taaaccacgc cgagcgaatt
       61 atgcaaatcg gcaaagtgcg gatgttcttc gacgcttttt tcaagccgcg cacggtattc
      121 ccccagtact ttttcgtctt tgccggcgcc gccaaacaaa aatttgagat aaaaaccgtg
      181 catggtttcg tcgagattcg gcgtttgtgt ggcttcgcga atatctttca aagttagtat
      241 cgccgccaaa aaatcattgt cctgtatctg gcgaatggct ttttcaaagc gaaaactgaa
      301 gaaacgataa ttaataaatt ccgtgcgggc gttcatcagt ttttgaagtt cattgatcac
      361 gcgcgtcggt ttgatggaag acggtgcaaa aacggcgctt tgaaaccgtt cggcgtgcat
      421 gcgcagaatc accatgccga tattgagatg cacttcaaga taattagggt tgatcgctaa
      481 tgcggtttcg taggtctgga gcgccgtatc caattcgcca ttgagcgcat aagcgagacc
      541 gaggctattg tgcaagtccg cgtagcgcgg ttccagttcg atggctttat gtaagacctc
      601 gatggctttt tgcgtttcct ttttttctac aaacgctaaa cccagattgc gataggactc
      661 gaccatttgc gggttgatgg ttatggcatt ttcaaactcg gcaatggcct catccaccaa
      721 acctttgtgc aaaaagagca agccgatctt attgctggag acggcgtgtt tgatggtttt
      781 gatgcgatca cggataaaaa aaagcaactc gatttcccac gcgatatcct gatgaaatgc
      841 cgctacgaca tcgcgaaaac gatcggtatc taaatattta tactgttccg ggtcgagttt
      901 tttttgatga acggtgacga tacggccgga atcaaaaatt tccgctttga ccaaaaggga
      961 ggataaatta ccggctgttt gtatatgaaa aacttttccg cctgcatata cgttgctatt
     1021 gacgcctatt gattccatag acacctctgc taataacatt tacatacgat tttaacatag
     1081 tgaaaacctg accactacgg tatgatatta ggcagcaggg cagcgcatac acaataccct
     1141 aagcagggta tattgaaaac ctgataaaaa gggtaaaatg atgaaattat atggaagctt
     1201 tttcggagag gcgtgtcttg attttttcct gaaatgcaag aagtgctgcg gcgtcgaaga
     1261 tctgaatttt gagacctgcg atgataccta cgccgtcaaa gacgggctga taaacgatgg
     1321 tcacttcctg ttgcccatac gcagcgtttc gttgtatcgg ttgtccgtca agtgcgcgat
     1381 aaaacgcttc ctgcatgggg agatcttgtt cgaatagttt gaacatgctt tggccttgcc
     1441 attcgctgcg gtacgagggc agcatcagtg tgcggtcccc ttcgatctgc gtgatgacgc
     1501 cataagcgtc gcagtcaaag gtaaagagcg gttgcggcgc agagcgcata tcttcggccg
     1561 cttcggctaa aagcatatct tcgatggaat aacctacgca taaaatcgtc agcgctccgc
     1621 cgatgatgcg cgtctccatg tgggtggctt gtccgtcaat aaggaaacga aaggcggtat
     1681 gaacgggatg ttccggtaaa gcctcttgta tacggctttc aaaagccggt tgatcatcgg
     1741 ggtgaataag ctgacggata tcataaccga tcgtcgtgta acgcggttta ttaaaacgtt
     1801 tttcaaaagc ggtattcaca gactgaatgg ccatttcccg gttgatcgta aaagcccact
     1861 gcgggatttc ttccagtgca cccttgagtg cgatttcagg aacaggtact tcctgcatca
     1921 cagggatgat ttccggcggc gctttgaagg caatgatacg cgatagatcg tcgccttcgg
     1981 tcaacgacga cgccgacact tcaaattttt gcccttccat ataacgttcg ttgcgcacga
     2041 gtatggtaaa aacttcaccg gagcgggcgc gttcgagcag tttttgattg tcaatgggtt
     2101 gctcgctgcc ttccataaat acgccgtgaa tgcccgacca caggctgagc gacgttgggg
     2161 tcccggccgt tgtcatgata tcgcgcgcca catcgttttg aaaaggaaac tggcccgccg
     2221 cgtccacgat catcgcaccg tcgtgcgccg tttgtacgat ggtttcgatc atacgaaact
     2281 gacgttcgat cgtgtcctga agttctttgt tttttcgatc ggctgtttct tgcgaacgtt
     2341 tttgtttggt gacttcgtga atatggatgg ccgtgccgtt gactttgtta ttgaccaaaa
     2401 ccgggttaaa tgacacttcg gcgagcacaa gccggtcggg taattgaaac tgctgttcga
     2461 tcataaattt ttcaccgcgc atcgcacgcg taagtacgat cacccatgcc gcctgggcat
     2521 ccggcttaag atgatcgatc atattggaac cgggatgcag ttcgatctga tggcggagat
     2581 gaaacagccg tttaaacggc tcgttgatca gcagtacttt gaaatgttca tcgaccgtac
     2641 acaccccatc cggcgtattg cctaaaatgc cggatacatt ttcattgagc cggtacaaaa
     2701 gtaacgcgag ctttttattg cgttgcagac tgtaacggta aaaaacggcg ataacgctga
     2761 tcagaatcgt gatcgcgctg gcgatgatga gaaatttgat cgccgtgtgc cagtcctgac
     2821 taagggattc ctgataacgg atactacgcg tgcgaagttt ttcagcggag ataccgagcg
     2881 attcggcggc acgatgaatt tcgtcaatca tttcgccggt gaggttttct acccggatgc
     2941 tatcgggaat gatcgaggtg cgtttgaatt tacgtttatg aatacgctgg tagagattgt
     3001 caatacgtgg caatgcattg cgcatatcaa ataccgaccc atccgtccaa tctacgccga
     3061 gcgaggcttg ccaggctttt tgatagatac ggttccagtc atcaaaactc gtgtcggtgg
     3121 ccgctaaaaa ttgatcggaa ataacttctt caagaccgta cacggcttgt tgcagtttat
     3181 atatacgggc gattttatcg gtatgcgatt gtatttgcgt ctgattttcc gtcatggagc
     3241 gcatgaggaa aaaacaccaa acgatgaaag gaataggaaa cagcgcgaca aacgccataa
     3301 ataaccggtt acggacttgc tccgtcatgc catatctcca tctgagcgcg ggagtcggat
     3361 cgtcggggtg cgcgtttaaa ttttgattcg gtcggaatca aaagtaacga agaaacttgt
     3421 ttttcaaaag taaaatcttt agttatgcgt aattatttta acgaaaggaa aacgtttgga
     3481 tacgcacgaa acatggatgg cgatggcgtt taaagaagcg gaaaaagcgt acgctaaaaa
     3541 tgaaattccc gtcggcgccg tgattgtctt ggaaaaccgc attatcggac gcggtcacaa
     3601 tcaggtagag atgcttcagg atccgacggc gcatgcagaa atgatcgcga tgacgtcggc
     3661 cgtggctacg atcggttcca aatggttgta cggcgctacg atgtacgtta cgctcgaacc
     3721 gtgctccatg tgtgccggtg cgctggtgct gtcgcgaatg agtcagcttg tttttggagc
     3781 gggcgatccc aaaaccggcg cctgcggttc gctgcataat attgtgcagg atgaacgtct
     3841 caatcacagg atcgaagtcc ttcgcggggt tatggatgcg aaatgttcgg ccatactcaa
     3901 ggattttttt gccaagctac gcaaacccgc cccacaggat aattaagcaa acccacgtcg
     3961 tttttttctg catgaaccca atcccatact catggggttg tgccctccga cacttggctg
     4021 tatcttacag cacgcaagtt ttcatgtctt caaaaataat tctaatggtt ctcatttcgt
     4081 gaaggaggtc gtatgacaat tcgaagcgtg ctcttatggt gttgttttgt tctgtgttta
     4141 cccgcagtgc aagcgcaaaa agtcaaactc aaaaccggtt cggtcaaaga aaaaaaagac
     4201 gaaaagaagg aaaacaaaac tgaaacgaca tcatccggta cgttcaaagt gggcgatgta
     4261 gccgaaggct tggaaacgga tggtaaatgg tataaagtgg atattcttaa agttgaagcc
     4321 gataaatatt ttattcactg gcaaggctat aagtcaacgt atgatcgctg gattgaagcc
     4381 ggtaaggtga gggcaatcgg ttcgtccgga agtgcctccg ctaacgcgag tacgtccgcg
     4441 gcttctaccg cttcgggagg taaaaccaag gtgtacggta ccggagacgg aggattcaaa
     4501 gtaggcgaat atgcggaagt gctcgaccgt gacggaaagt ggtatgaagc ggaaattcaa
     4561 aaagtgaatg aaggtcaacc gaacgagtat ttcgtgaaaa tcgaaaacta taacggattt
     4621 tgggtgaaag aatccaacgt gcgggcgaaa agctccggta catccagcga gcctgtcgga
     4681 tttaaggttg gcgacgtcat cgaggccgtg gattttggtc gatggaataa aggaaccgtc
     4741 caaagtattc gcaccaccaa cagcgggaat aagatgttta cggtggcctt tgataacgga
     4801 ggctctaaag aatttacaga aaataaactc cgcgtcatac cggtgtataa tttcagcggg
     4861 tccaattata aaacgggtca atatattcgt atatggtaca gcggcagaga ttcgttggac
     4921 gcgccgatcg tggacatcaa agataacaaa ttactcatca actacggcgg atatggcgcc
     4981 aagtggtttg attttgatgc aaaagaaatt agaaatgatg cgcgtaccgc ggccatcgcg
     5041 gcatccaaag ccacggaaga acagtttttt caagattgta acaaatattc cgaagcgata
     5101 aggatattcg gttatcttta caataaaaaa catgacacgc ggtttgtact gtacaatcag
     5161 ggtttgactt caccgcgtcc ctccgacgtc gggccgatgc tcaaacagat gaatgaactg
     5221 gatgcgctca tcaaaagcaa ataccctaat attcagaata ccgaacaaac atggactacg
     5281 gaactgtcca aaaatcccgg acattggcgc gtgatcgccg aagagcgaaa tgaattggct
     5341 cagagcgtcg tgacggatat gatcaaagac aagatcagtg aagcgacccg aattttgact
     5401 aattatgaat tcctgaaaaa tcatgaaatc ggcgaatttg aagcaggtaa aggaatcggc
     5461 gttacatcaa gccttcagga cattttggat aataacggcg ccgattttat gaaaaaagaa
     5521 gaagaaaaaa tagccgatgt tatcaaagcg gcgcaggaag ctaatctcgg tcaaaatgaa
     5581 ataacggcgt ttcgggaatt cctaaaaaaa ttatccgtcg atgtgaagag caaattccct
     5641 tcgtccctcg tatcgtcgca atttaatccg gcattgcatg atgcggctgc agaggcctcg
     5701 gctaaagcgt atatccaaaa aacttatagc ggcgcggcta ttttgaaaat cggtgttatc
     5761 ggtaatcagg ttatcgaaaa aaataatctg ggaataccgc tgtaccgcta taaaagagtc
     5821 ggtgtactat tcagagattc ccaaggtcgt tgccgttttg tcccggcgta ttacagagaa
     5881 gactatgcgg gcggaggagc gtatggtacg ccctacatcg agcatcactt tacgtactac
     5941 caaagctgca attgagtatt tctgaaaatt attttcaaaa aaacaaaaga cctgctcttt
     6001 caagcaggtc tttttattgg attcaattat atagcgcagc gcgtgaaatt tttatttgcc
     6061 ttcgcgtttc attttttcag aaaagtattc gatcgttttt agcaatccgg tttcgatatc
     6121 tacggtaggc tcccagttca aaagccgttt ggctttgctg atatccgggc ggcgaaccgt
     6181 cggatcgtct ttaggtaaag gacgatatac gatttcactt ttgctattgg tcagtcggat
     6241 gataagttgt gcaaactgaa gcacggtcat ttcatgtgga tttccgatat tgacgggata
     6301 gtgttcgctg gattgactca ggcgatagat gccgtcaatc agatcgctgc aatagcagaa
     6361 agagcgcgtt tgtttccctt cgccgaaaac cgtaatgggt tggttgcgaa tggcttgatt
     6421 gataaacgtc ggcacgacgc gcccgtcatt agggcgattg cgcggcccgt aggtattaaa
     6481 aatccgtacg atgcgcgtat caacgttatg aaatgtatga taggccatcg tcatcgcttc
     6541 ggcaaatcgt ttggcttcgt catatacgcc gcgcgggcct accggattga cgttgcccca
     6601 gtactcttca tgttgcggat tgatcaacgg atcgccatat gtttccgaag tcgaggcgag
     6661 caaaaaagcg gcgtctttac ttttggccaa acccagcgct ttatgtgtcc cgagcgaacc
     6721 cactttaagc gtctgaat
//
LOCUS       DAOQNQ010000184         6662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_4417_length_6662_cov_5.257606, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000184 DAOQNQ010000000
VERSION     DAOQNQ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6662
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4417_length_6662_cov_5.257606"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2230)
                     /locus_tag="PK754_11000"
     CDS             complement(<1..2230)
                     /locus_tag="PK754_11000"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HNB10077.1"
                     /translation="MKKASPKKADAAMLNGVNYEGAKGLRFNRFFTKENIHPFDEIVW
                     EKRSSVITEPDGRVVFEMRDLEIPREWSQLATDIAASKYFRKAGVPGTGREISVKQLV
                     HRVAHTIRIEGEAMGYFDSSADAEVFELELTHLLVNQKGAFNSPVWFNCGLYHEYQIK
                     GSGGNFYWNAQSGKIEMTEDSYSKPQCSACFIQSVSDDLMGIFELAKNEAKLFKYGSG
                     TGSNFSSLRGKQEKLSGGGTSSGLMSFLTVLDRGAGATKSGGTTRRAAKMVCLDMDHP
                     EIVDFINWKVKEEKKVKALIDAGYESDFNGEAYQTVSGQNSNNSVRISDKFMNAVLEN
                     GKWDTIARTTGKTVETHDARNLYEQIAYAAWACADPGVQFDDTINDWHTCPNTDKIHA
                     SNPCSEYMFLDDSACNLASINLMKFADESGKFDIEGFRQSCRIFIIAQEILVDMSSYP
                     TQQIAQNSHDYRPLGLGYANLGTLLMVNGLAYDSEEGRAMAGAITAIMHAWGFRGSAE
                     VASIKGPFAGYKKNEKPMLRVMNKHRDAAYKINPEFSHDEALLKAAQEDWDTCVKMGE
                     KYGYRNAQATVLAPTGTIGLLMDCDTTGVEPEFSLVKWKKLAGGGYFKIVNNSIPQAL
                     RNLGYSADDIDGIIKYILGHGTFEGAPYVNPEQLKQMGFADAEIAEAEAYVQKNKSLD
                     EWTPHINIKSLKTKGLSGEQISRAVIYIGGAQTVEGTHLLKEEHLAVFDCANKCGIGK
                     RFI"
     gene            2968..3663
                     /locus_tag="PK754_11005"
     CDS             2968..3663
                     /locus_tag="PK754_11005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HNB10078.1"
                     /translation="MQVPFSTLVTSSVGKKIMTGLTGLGLCLFILSHLLGNFNLFLGP
                     DAFNQYTKTLESFGKALYVIEALLVLAFVMHAVVGISVWQSKRRARPEAYHKTAYAGG
                     NSKKSFASLSMLYTGLIILLFLFIHIKSLKFGPNMAEGYVYTHTVTGEKMRDMYKLTV
                     EHFKNPFYAFGYALVMLLLGVHLRHGFWSAFQSLGLTHQRLKPLIFSVGILFASAMAL
                     GFFVLPLWIYFSR"
     gene            3722..5635
                     /locus_tag="PK754_11010"
     CDS             3722..5635
                     /locus_tag="PK754_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HNB10079.1"
                     /translation="MKLDSKIPSGPLADKWSQHKFNMKLVNPANRRKYTVIVVGTGLA
                     GASAAATLGELGYNVHSFCIQDSPRRAHSIAAQGGINAAKNYSNDGDSVHRLFYDTIK
                     GGDYRAREANVHRLAELSVNIIDQCVAQGVPFAREYGGQLDNRSFGGAQVSRTFYARG
                     QTGQQLLLGAYGSLMRQVNEGAVKMFPRREMLDLVVIDGQARGIVVRNLVTGAIESYA
                     GDAVLLCTGGYGNVFYLSTNAKNSNVTASWRCYKKGAFFGNPCYTQIHPTCIPQSGDY
                     QAKLTLMSESLRNDGRVWVPKTKGDKRPPEQIPENERDYYLERKYPSFGNLVPRDVAS
                     RNAKSVCDEGRGVGETGLAVYLDFSDAIKRLGEDDIREKYGNLFDMYERITGDNPYKT
                     PMRIYPAVHYTMGGLWVDYNLMSTVPGLFVLGEANFSDHGANRLGASALMQGLADGYF
                     VIPYTLANYLASSSFNKITTDHQAFKTAATAVTEQTKKLLAIKGKKTVLEFHRELGKI
                     MWDEVGMARNKAGLERALSEIPKLREEFWKNVNVPGEGELLNKNLEFAGRVADFLELG
                     ELMAMDALQREESCGGHFREEHQTPDGEAKRNDEQFAYVAAWEYAGADKAPILNKEEL
                     TFENVHLAQRSYK"
     gene            5684..6439
                     /locus_tag="PK754_11015"
     CDS             5684..6439
                     /locus_tag="PK754_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNB10080.1"
                     /translation="MEQKKMTLHLKIWRQKNMQSAGKMVDYTLDNVSTDMSFLEMLDV
                     LNERIIKNGEDPIEYDNDCREGICGMCSMVINGVAHGPEKGTATCQLHMRKFKDGDHI
                     TVEPWRASPFPVVKDLATDRSAFDAIIAAGGYTSCRVGSAAEANALLVPKDMADKAMD
                     AAACIGCGACVAACPNGSASLFVSAKIAQFTYLPQGHAERKQRVLNMVNKMDELGFGD
                     CSNHGECEAVCPKEISITHIAIMRKEYLKAALS"
BASE COUNT         1731 a         1648 c         1501 g         1782 t
ORIGIN      
        1 caataaaccg tttcccgata ccgcatttat tggcacaatc aaatacggcc aaatgttcct
       61 ctttgagtaa atgcgtgcct tcgaccgttt gcgcaccgcc gatatagatc accgcacggg
      121 agatctgttc gccgctgagg cctttggttt tgaggctttt gatattgata tgcggcgtcc
      181 actcatcaag cgatttgttt ttctgtacgt aagcttcggc ttcggcgatc tccgcatcgg
      241 caaaacccat ttgtttcagt tgttcgggat tgacgtacgg cgcaccttcg aacgtaccgt
      301 gaccaagaat atatttgatg ataccgtcaa tatcatcggc ggagtaaccg agattgcgca
      361 atgcttgggg gatcgaattg ttgacgattt tgaaataacc gccgccggcc aattttttcc
      421 atttgacgag cgaaaattcg ggttccacac ctgtcgtatc gcaatccatc agcaatccga
      481 tcgtaccggt cggagccaac acggtagctt gggcgttacg gtagccgtat ttttcgccca
      541 tcttgacgca ggtatcccaa tcttcttggg ccgccttcag caaggcttcg tcgtgactga
      601 attccgggtt gattttatat gcggcgtcac ggtgcttatt cattacacgc aacatgggtt
      661 tttcattttt cttataaccc gcaaaaggtc ctttgatgga agccacttcc gcagaaccgc
      721 ggaatcccca tgcgtgcatg atcgccgtga tcgcaccggc catcgcgcgg ccttcttcgg
      781 agtcgtatgc aagcccgttg accatcagga gcgtaccaag gttggcataa cccaaaccga
      841 gcgggcggta gtcatgactg ttttgcgcga tctgctgcgt cggataactg gacatatcca
      901 caaggatttc ctgcgcaatg ataaaaatgc ggcacgattg acgaaaacct tcgatgtcaa
      961 acttacccga ttcatcggca aacttcatca ggttgatgga agcgaggttg caggcggagt
     1021 catcgaggaa catgtattcc gagcagggat tggatgcatg gatcttatcc gtattgggac
     1081 acgtatgcca atcattgatc gtatcatcaa actgtacgcc cgggtcggcg catgcccagg
     1141 ccgcataagc gatttgctca tacaagttgc gcgcgtcgtg ggtttccacc gttttacccg
     1201 tggtgcgtgc gatcgtatcc catttgccgt tttccaaaac ggcattcata aatttatcgc
     1261 tgatacgtac ggaattattg gagttctgtc cggacaccgt ctgatacgct tcgccgttga
     1321 aatcggattc gtatccggcg tcaattaacg ctttgacttt tttctcttct ttcactttcc
     1381 agttgataaa atctacgatt tcaggatgat ccatatcaag acagaccatc tttgccgcac
     1441 ggcgcgtcgt gccgccggac ttggtagcac cggcaccgcg atccagaaca gtcagaaagg
     1501 acatcaaacc ggacgatgta ccgccgccgg aaagtttttc ctgcttaccg cgcaacgaag
     1561 aaaaattaga acccgtaccg gaaccgtatt tgaaaagttt agcctcgttt ttggctaatt
     1621 caaaaatacc catcagatcg tccgacacgg attgaatgaa acacgccgag cattggggtt
     1681 tggaatacga atcttccgtc atttcgattt tgccggattg cgcattccag taaaaattac
     1741 caccgctgcc cttaatctga tattcatgat aaagaccgca gttaaaccat accggtgaat
     1801 tgaaggcgcc tttctgattc acaagcaggt gcgtaagttc caattcaaaa acttccgcat
     1861 cggccgacga atcaaaatac cccatcgctt cgccttcgat gcgaatcgta tgggccacac
     1921 gatgaacaag ttgtttgacg ctgatttcgc gtcccgttcc cggcacaccg gctttacgga
     1981 aatatttgga agccgcgata tccgtagcca actgagacca ctcgcggggt atctccaggt
     2041 cacgcatttc aaatacgacg cgaccatcgg gttcggtgat cacgctggaa cgcttttccc
     2101 acacgatctc atcaaatgga tgtatgtttt ctttggtaaa aaagcgatta aatcgaagac
     2161 ccttcgcgcc ttcataatta acgccattga gcatcgccgc atccgccttc ttgggagatg
     2221 cttttttcac atcacgctct tgaattgcca tggctccaag attcctttag aataaggttt
     2281 gataaacagt tataaaaaac ttattaaaaa cgttttttcc tgtcttacga gccgtctgta
     2341 gaagggacaa aagaaccgta ggtctgaatc gtcttttcag acaaccttcc cgatgtccac
     2401 cggcctctca actcctgtgg acgaaccgaa agccatgtcg ctctcaaaaa caggggtaag
     2461 cacataataa agtcccacct aaaaaaacgc aagccgaatt tttgcatttc aagataataa
     2521 caagttatca acatgataaa tatttatatt tattaactat aaataataac gatatcgaat
     2581 tgccaacaat tttgttaata aaaaattacc aacattatct aaacaaaaaa cgaagttaat
     2641 tttgaatgat tatttattaa gattttacgt gctgtttgtg ctcaaaaaag accaaatagc
     2701 taaaaacctg aagaaatcat aagaaatcgt tattataaaa ttcaccatca ctcatgtaaa
     2761 ctattaaagc catgcgttca tttaaaagcc aatgatcatt attttatcgg agcatcttat
     2821 ccagcgaagc aataatcgta gcgcaagttt aatcaataca atccccttgc attttttttt
     2881 tcattcccgt attttgcctt tgttcattcc gacagcaatt cgtttcttca taatatcatt
     2941 atttaccacg tttccggagt ttactatatg caggttccct tttcgacgct ggtaacctcc
     3001 tccgtcggca aaaaaatcat gaccggcttg acggggttgg gtttgtgtct ttttattctt
     3061 tctcacctcc tcggtaattt taatcttttt ctcggtccgg atgctttcaa tcaatacacc
     3121 aaaaccctgg aaagtttcgg caaagcgctg tatgtgatcg aagccttgct cgttttggcc
     3181 tttgtaatgc acgcggtcgt aggcatctcc gtatggcaga gcaaacggcg cgcgcgtccc
     3241 gaagcctatc acaagacggc ttacgccgga ggcaacagca aaaaatcctt tgcttcgctt
     3301 tcaatgcttt ataccggtct tattattctt ctgtttcttt ttattcacat caaatcgctt
     3361 aagtttggac ccaatatggc cgaagggtat gtctacaccc acacagtcac tggcgaaaaa
     3421 atgcgagaca tgtacaaact gacggtcgaa cactttaaaa atccgtttta tgcgttcgga
     3481 tatgcgttgg taatgctgct ccttggcgtc cacctccggc atggattttg gagcgccttc
     3541 caatcgctgg gcctcacgca tcagcgtttg aagccgctta ttttttcggt gggaattctt
     3601 tttgcctcag cgatggcgtt gggctttttt gtgctcccgc tgtggattta tttttcacgg
     3661 taggacatgc tttacgataa tatctgcatc ctaatgacta tataagctta cgaggatact
     3721 catgaaactt gattccaaaa ttccctcagg cccgctcgcg gataaatgga gccaacataa
     3781 gttcaatatg aagttggtca atcctgcgaa tcgccgtaaa tatactgtca ttgttgtcgg
     3841 tacgggactt gccggtgcat ccgctgcagc aaccctcggc gaactcggct acaatgtaca
     3901 ttctttttgc attcaggatt cgccccgccg agctcactcg atcgcggcgc agggcggcat
     3961 caatgcggca aaaaattatt ccaacgacgg cgacagcgtg caccgtttgt tttatgacac
     4021 gatcaaaggc ggagattacc gcgcacgcga agctaatgtc catcgtttag cggagctttc
     4081 cgttaatatt atagaccagt gcgtcgcaca aggcgtcccg tttgcacgtg aatacggcgg
     4141 ccaactagat aaccgctcct ttggcggtgc acaggtatcg cgtacatttt atgcccgcgg
     4201 ccaaaccgga caacaacttc tccttggcgc ctacggttcg ctgatgcgtc aggtcaatga
     4261 aggcgccgtc aaaatgttcc cccgtcgaga aatgctggat ctggtggtga ttgacggtca
     4321 ggcccgcggc atcgtcgtcc gcaatctcgt gaccggtgcg atcgaatcct atgccggtga
     4381 cgccgtactg ttgtgtaccg gcggctacgg aaacgttttt tatctttcga ccaatgctaa
     4441 aaattccaac gtcaccgcgt cgtggcgatg ctataaaaaa ggtgcgtttt tcggcaatcc
     4501 ctgctacaca cagattcacc cgacctgtat cccccaaagt ggcgattatc aggccaaact
     4561 gacgctcatg agcgaaagcc tccgtaatga cggtcgcgta tgggttccta aaaccaaagg
     4621 tgataaacgt ccaccggaac aaattcctga aaatgaacgc gactattatt tggaacgtaa
     4681 gtacccgagt ttcggaaatc ttgtaccgcg tgatgtggca tcccgtaatg ccaaatccgt
     4741 atgcgatgaa ggacgcggtg tcggcgaaac aggtctcgcc gtatatcttg atttttcaga
     4801 tgccatcaaa cgcctcggtg aagacgatat tcgtgaaaaa tacggcaacc tttttgatat
     4861 gtacgaacgt atcaccggtg acaatcctta caagacaccg atgcgtatat atcctgctgt
     4921 tcactatacg atgggcggcc tttgggtgga ttataatctg atgtccaccg ttccgggctt
     4981 gtttgtattg ggtgaggcca atttctcgga tcacggagcc aatcgccttg gcgccagcgc
     5041 cttgatgcaa ggactcgccg acggctattt cgtgattcct tataccttgg ctaactatct
     5101 cgcctcgtca agttttaata aaatcacgac ggatcaccag gcgttcaaaa cagccgcaac
     5161 cgcagtaacc gaacaaacga aaaaactatt ggccatcaaa ggcaaaaaaa ccgttttgga
     5221 atttcatcgc gaactcggca aaatcatgtg ggacgaagtc ggtatggctc gcaataaagc
     5281 aggattggaa cgcgctttaa gtgaaatccc aaaactccgt gaagaatttt ggaaaaatgt
     5341 caacgttccc ggcgaaggtg aattactcaa caaaaacctc gaatttgcgg gacgtgttgc
     5401 tgatttcttg gaacttggcg agctcatggc aatggacgca ctgcaacgcg aggaatcatg
     5461 cggaggccac ttccgtgaag aacatcagac accggatggt gaggccaaac gtaatgacga
     5521 acagtttgct tacgtcgcgg catgggaata tgccggtgcc gacaaagcgc cgatactcaa
     5581 caaagaagaa ctgacgtttg aaaacgttca tcttgcccaa cggagctata aataatcgga
     5641 accgaagcgc cgcaacgcgg cacttaatag ataagaggaa attatggaac aaaagaaaat
     5701 gacccttcac ctgaagatct ggcgccagaa aaacatgcaa agcgccggta aaatggtgga
     5761 ttatacgttg gacaatgtat caaccgatat gtcgtttctc gaaatgctgg acgttctcaa
     5821 tgaacgcatc atcaaaaacg gtgaggaccc catcgaatac gataatgatt gccgtgaagg
     5881 tatttgcggt atgtgcagca tggtgatcaa cggtgttgcc cacggtcctg aaaaaggcac
     5941 ggccacctgc cagctccaca tgcgcaaatt taaggacggc gaccacatca cggtagaacc
     6001 ttggcgcgca tcaccctttc ccgttgttaa agatttagcc acggatcgtt cggcttttga
     6061 tgcgattatt gccgccggtg gttatacctc gtgccgtgtt ggctccgcgg ccgaagccaa
     6121 tgcgttactg gttcccaaag atatggcgga taaagcaatg gatgcggccg catgtatcgg
     6181 ttgcggcgct tgcgtagcgg cctgccccaa cggctccgct tccctttttg tatccgctaa
     6241 aatcgcgcag tttacttatc tgccccaagg tcacgccgaa cggaagcaac gcgtactcaa
     6301 tatggttaat aaaatggatg aactcggttt tggcgactgc tccaatcacg gtgaatgcga
     6361 agctgtttgc ccgaaagaaa tatccatcac tcatatagct atcatgcgca aagaatatct
     6421 taaagccgca ctgagctaaa tgctaataat cttgcccctt tgaaaaggcg agcgatgcat
     6481 gctcgccttt tttgtaatac tatttttata attttttaat ttttttgtga aaccttaaac
     6541 cataagcatc gtattcatta ttggttacac ccttaatgaa aggtggtttg tactatggca
     6601 ggtgtttata aattctcaga catgcgcagt cccctgcttt ttgtttccct gcttttttcc
     6661 gc
//
LOCUS       DAOQNQ010000185         6594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_4489_length_6594_cov_6.388133, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000185 DAOQNQ010000000
VERSION     DAOQNQ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4489_length_6594_cov_6.388133"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1392
                     /locus_tag="PK754_11020"
                     /pseudo
     CDS             148..1392
                     /locus_tag="PK754_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844442.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
     gene            1394..2629
                     /gene="nosD"
                     /locus_tag="PK754_11025"
     CDS             1394..2629
                     /gene="nosD"
                     /locus_tag="PK754_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HNB10081.1"
                     /translation="MLKWIIHSLLAVTLGMTVFAKTLHVGEGFSYSSVRSALDAAKNT
                     DTIIVHKGRYAEGQLLIQKQVVLIGVDSPVIDGLTTSEIWRIEASFVTVRGFVFVRAG
                     ISYTQENAAVKVNDARFVTIEDNRFEKNFFAIYLAKSSFCVVRNNFISSDGKSESSSG
                     NGIHLWYCRAITIENNSIYGHRDGIYFEFVKSSIIRNNHSLGNLRYGLHFMFSDSCTY
                     TGNTFEENSAGVAVMYTRYIAMYGNTFRRNWGPASYGILLKDITDSDIRDNVFDNNTA
                     GVHMEGSNRMTMRRNRFVHNGWALRLLGNCTDNFFTENDFENNAFDVATNSRSNRNTF
                     SMNYWSNYSGYDLDRDGVGDEPYRPVSLFSVISEQHTEAMILMRSLTIVALDLVERII
                     PSLTPAALLDDKPKMGRCL"
     gene            2626..3360
                     /locus_tag="PK754_11030"
     CDS             2626..3360
                     /locus_tag="PK754_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB10082.1"
                     /translation="MITIQSLTKNFGEQRVLNRVNASFDTGKVTAIIGPNGSGKSTLI
                     KSILGLVTPDSGLITLDGKAIAPQDAEYRRRIGYMPQTARFPENMKVHELLSLMADLR
                     DMVTDRDEELMRSLQLEREISKALKHLSGGTKQKVNAYLAFLFRPDVLILDEPTAGLD
                     PVSSTLLKDKILKEKNNGRTVIITSHVMSELEEITDRVLFLNEGCVNFEGTIEDLRQK
                     TGEERLERSIAHLMKGLGLWTLSKKY"
     gene            3336..4127
                     /locus_tag="PK754_11035"
     CDS             3336..4127
                     /locus_tag="PK754_11035"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNB10083.1"
                     /translation="MDALQKILKFEWYDQIRNKWIVLYTAFFFVLTFGLFQLQGDASR
                     VFLTMTQILLLIVPLVSMIFGAMHVYHSREFIEILLCQPIRRSKLFWGVYLGMALPLA
                     GGFVVGAGVPILMFGYNSIPDWRIPVMLLGTGLLLTMIFSALAFGIAMRTDDKLKGIG
                     FALGLWFALALLFDGMVLYIVYAFADYPLEKPLIGISMLNPINIARSMILMKMDVAAM
                     MGYTGAAFKKFFGGSLGVIVSLAALVGWAVIPLFFAKRHFLKKDF"
     gene            4131..4640
                     /locus_tag="PK754_11040"
     CDS             4131..4640
                     /locus_tag="PK754_11040"
                     /inference="COORDINATES: protein motif:HMM:NF017262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNB10084.1"
                     /translation="MLTGYFISVFLHILCAMIWVGGLIFFVIVFVPLLRNPEYKNVAP
                     KIVLWAGERFRFWGWIVFGLLLTTGLYNVFARGYSWGDFFKSEFYDSHFGHTLGIKIT
                     LFMLLLVLGGLHDFWLGPKATRMWRDTPDHPSVPGLRKAASWIGRVNLLISLMIVFLA
                     IMIVRGNPW"
     gene            4642..5013
                     /locus_tag="PK754_11045"
     CDS             4642..5013
                     /locus_tag="PK754_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10085.1"
                     /translation="MRIFNIKIPALLLVVTLFSAGLPQAFRAISENNIKTCCCLSQGK
                     ACTCDHKNVTQADAGACSMRNEGCGTKGNGEMQRESLIAVHIQPAIVIAEFHYTTILS
                     SFTQPIFISKTPSDIFHPPRA"
     gene            5265..6056
                     /locus_tag="PK754_11050"
     CDS             5265..6056
                     /locus_tag="PK754_11050"
                     /inference="COORDINATES: protein motif:HMM:NF024515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNB10086.1"
                     /translation="MLRWLAIIIMTAFFISSCEKDKNSSDPVNDMVLIGQGYDQGVLV
                     SVYSEDTLTTGYNAIYTRLQDSASGEILDEGHIHLSPLMHMTEMTHAAPYGNPESEEA
                     EEGLFEGYVVFQMPGNDMEYWELEVEFHDHHSGKEGSVMIEPKVKSSNRCKVVTGTDA
                     KSYILSWKKPRVPEVGLNTLELMVHERVSMMSFPDVTDATLAIEPTMPSMGHGSPNNV
                     DPVYVSGAVYRGKVNFTMTGDWQVDVSITRNAATVVETHFDVTVD"
     gene            6145..>6594
                     /locus_tag="PK754_11055"
     CDS             6145..>6594
                     /locus_tag="PK754_11055"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNB10087.1"
                     /translation="MKPLLMIIKYCTGLVVLVTLSVQAQEHATMVHGRIVDEETREAV
                     RYAHIRMDSTEEGTIADSAGYFHLPRHHGKYKLRISCVGYQTAWVSVDHFSEKRIMPV
                     FLRSNAIRFEEEVIVSAPWIEQQQESYYLEKKLNTTDDILEKTDGLSL"
BASE COUNT         1700 a         1419 c         1636 g         1839 t
ORIGIN      
        1 atattaaatt agtttaatta tttacagtat tgattattat tattattata atgcgagcat
       61 cgtaataata ctaaataaaa tatgctaaat aatatatata tatatatata tatatatcaa
      121 taaaatcatt atccctgtta tttatagcta ctgattgatt ttttgacgga cagcggtacg
      181 tcggccatga gtcataaaca atgggccgct atgatcagcg gcgacgaatc ctatgccggc
      241 gcacaaagtt gggaacgtct ggagtccgtc gtcaaagacc ttaccggata tcctcatatt
      301 ctgcctacgc atcaggggcg cgctgcggaa cgtattctgt acggtcatct gggcggcaaa
      361 ggtaaagttt ttttgagtaa tacgcacttt gacacaaccc gggccaatat cgaattttcc
      421 ggcgccacgg cgatcgattg ccccatcccc gaggcgctgg agccgggtaa accgcatcct
      481 ttcaaaggta atattgattt ggtgcaggtc gagtattgga tcaaaaaaca tggcgccgaa
      541 aatattgccg ccgttatcct gaccgtcacc aataataccg gcggcggcca acccgtgagt
      601 cttgccaatg cccgcgcttt gagtgaactc tgcgcgaagt acaaagtttt gtttatcata
      661 gatgcctgcc gcgtcgcaga aaatgcatac tttatcaaac atggcgaagc ggagtgtgcg
      721 catctttcat ataaagaaat tgcacaagcc atgtttgatc ttgccgacgg ttgtgtgatg
      781 agcgccaaaa aagacgggct cgtcaatatc ggcgggtttc tggcgttgcg ggacgatgcg
      841 atcgccgcag cttgtaccaa tcttttgatc attaccgaag gttttaccac atacggcgga
      901 ctagccggtc gtgatatgga cgctatggcc gtgggtttgc aggaagtttt tgatgcggat
      961 tatctcaagt atcgcattcg cagcacacag tatctcggtg aaaaaatcaa tgcgctcggc
     1021 gtgccgatca tgctacccgt cggcggtcat gcggtgtata tcaatgccaa agacctgtat
     1081 ccgcatatcc ccgtaaatca ataccccgga caagcgcttg tttgcgaact ttacaaagtc
     1141 ggcggtattc gttgcgtgga agtgggttcg gtgatgtttg gcaaatacga tgcgtccgga
     1201 aaattgattc cttcgccgat ggaactcgta cgccttgcga ttccgcgacg tgtatatacg
     1261 caaagccacg tggattatgt gatcgaagtt ttcgatgaaa tcgtaaagaa taaagaaaaa
     1321 gtacgcggtt ataaaatcac gtacgaaccg aaatttttgc gtcattttac ggcgcatttt
     1381 gaaaaactct gatatgctaa aatggatcat acatagtttg ttggccgtga cgctgggtat
     1441 gacggttttt gccaaaacct tgcacgtcgg agaagggttt tcgtattcgt ccgtgcgatc
     1501 ggcgttggat gcagcgaaaa atacggacac gatcattgtt cacaaaggcc gttatgccga
     1561 ggggcagctt ttgattcaaa aacaagtcgt actgatcggc gtggattcgc cggtcataga
     1621 cggtctcacc acatcggaaa tctggcgcat cgaagcgtcc ttcgtgaccg tacgtgggtt
     1681 tgtgtttgtc cgtgcgggta taagttacac gcaggaaaat gccgccgtca aagtcaacga
     1741 cgcacgtttt gtgacgatcg aagataatcg gtttgaaaag aatttttttg cgatctatct
     1801 tgccaaaagc agtttttgtg tggtgcgaaa taattttata tccagcgacg gcaaatcgga
     1861 atcctcgtcc ggcaatggca tccatctctg gtattgtcgc gcgatcacga tcgaaaacaa
     1921 ttctatctac gggcatcgcg acggtattta tttcgaattt gtcaaatcca gcatcattcg
     1981 caataaccat agtttaggca atcttcgcta cggattgcat tttatgtttt ccgacagttg
     2041 tacatacacc ggtaatacat ttgaagaaaa ctcggccgga gtggccgtga tgtacacacg
     2101 gtatattgcg atgtacggta atacatttcg ccgtaactgg ggtccggcat cgtatggtat
     2161 attgctcaag gacattaccg atagtgatat tcgtgataat gtgttcgaca ataataccgc
     2221 cggcgtacac atggaagggt ccaatcgtat gacgatgcgg aggaatcgtt ttgtacacaa
     2281 cggttgggcg ttgcggcttt taggaaactg tacagataat ttttttacgg aaaacgattt
     2341 tgagaataat gcgtttgacg tagcgaccaa ttcgcgcagt aaccgcaata cgttttcgat
     2401 gaactattgg tccaactaca gcggttatga tctggatcgc gacggagtcg gtgatgagcc
     2461 gtatcgtccc gtaagtcttt tttccgtgat ttccgaacaa catacggaag cgatgatcct
     2521 catgcgcagc ctcacgatcg tcgcgctcga tctggtcgag cgcattatcc cgtcgcttac
     2581 accggcggcg cttttggacg acaaacccaa aatgggacgg tgcttatgat aacaatccaa
     2641 tctctaacca aaaattttgg cgaacagcgc gtgctcaatc gcgtcaacgc ttcgtttgat
     2701 accggaaaag tcacggccat cataggacct aacggttccg gcaaatccac gcttatcaaa
     2761 tccatactcg gtttggtaac acccgacagc ggtttgatca cgctggacgg caaagcgata
     2821 gcaccacagg atgccgaata ccgtcgtcgt atcggatata tgccacagac cgcacgattt
     2881 cccgaaaaca tgaaagtgca cgaactatta tcgcttatgg ccgatcttcg cgatatggtt
     2941 acggatcgcg atgaagagtt gatgcgttcg ctgcagctcg agcgggaaat cagtaaagcg
     3001 ctcaaacacc tctccggcgg aaccaaacaa aaagtgaatg cgtacctcgc ttttcttttt
     3061 cggccggatg tactgatatt ggatgaaccc accgccgggc ttgatcccgt atcgagtacg
     3121 ttactcaagg ataaaattct caaagaaaaa aataacggcc gcacggtgat cattacgtcg
     3181 cacgtgatga gtgaattgga agaaatcacc gatcgtgttt tgtttttaaa tgaagggtgc
     3241 gtcaattttg aaggtacgat cgaagatctg cgtcaaaaga ccggcgaaga acggctcgaa
     3301 cgatccatcg cacatttgat gaaaggactt ggactgtgga cgctctccaa aaaatactaa
     3361 aattcgaatg gtatgatcag atccgtaata aatggatcgt attgtacacg gcttttttct
     3421 ttgtgctgac cttcgggtta tttcaactgc aaggggatgc aagtcgcgta tttctgacga
     3481 tgacgcagat tttgcttctc atcgtgccgc tggtaagtat gatattcggc gccatgcatg
     3541 tatatcactc gcgcgaattt attgaaattc ttttgtgcca accgatacgc cgcagcaaac
     3601 tattttgggg cgtgtatctc ggaatggcgc tgccgctggc aggagggttt gtcgtcggtg
     3661 caggtgttcc gatactgatg ttcggttata actcgatacc ggattggcga atccctgtga
     3721 tgctgctggg gacaggttta ttgcttacga tgatttttag cgcgctggct tttggtattg
     3781 cgatgcgcac agatgacaaa ctcaaaggca tcggttttgc tcttggcctg tggttcgctc
     3841 tagcgttgct ttttgacggt atggtgttat acatcgtgta tgccttcgcc gattatccgt
     3901 tggaaaaacc gctgatcggc ataagtatgc tcaatcccat caatattgcg cgctcgatga
     3961 tactgatgaa aatggatgtt gccgcgatga tgggttatac cggggctgcg tttaaaaaat
     4021 tctttggcgg ttctctcggt gttattgtat ctttggcggc cttggtaggg tgggcagtga
     4081 taccgctttt ttttgccaaa cggcatttct taaagaagga tttctaaatc atgttgaccg
     4141 gttattttat atccgtattt cttcatattc tttgtgccat gatttgggta ggcggtttga
     4201 ttttttttgt gatcgtgttc gttccacttc tccgcaatcc ggagtataaa aatgtggcac
     4261 cgaaaatcgt attatgggcc ggagaacgtt ttcgtttttg gggttggatt gtatttggtt
     4321 tgctgctcac caccggttta tacaatgtat ttgctcgagg gtattcctgg ggtgattttt
     4381 ttaaatccga attttatgac agccatttcg ggcacacact gggaatcaaa atcacactgt
     4441 ttatgctgct attagtactt ggtggtttgc atgatttttg gcttggaccc aaagccaccc
     4501 gtatgtggcg cgataccccg gatcatccgt cggtacccgg actgcgcaaa gcggcgtctt
     4561 ggatcggacg tgtgaatctt ttaattagtc tgatgattgt ttttttggcc atcatgatag
     4621 ttcgtggaaa cccgtggtga tatgcgtatt tttaacatca aaataccggc gcttctttta
     4681 gtggtaacac tattttcagc gggattgcca caggcttttc gagccattag cgaaaacaac
     4741 atcaaaacat gttgctgttt gtcgcaaggt aaagcatgta cctgcgacca taaaaatgtc
     4801 acacaagcgg atgcgggcgc ctgctctatg cgcaatgaag gctgcggcac caaaggtaac
     4861 ggtgaaatgc aacgcgaatc attgattgcg gttcatatcc aaccggccat tgttattgcc
     4921 gaatttcatt atacgacaat actctcctct ttcactcaac cgattttcat ttcaaaaacg
     4981 ccttctgata tttttcaccc gccgcgcgcc taaactcttt tccaaacaac ggatgctcgg
     5041 tgcatcgttc tttccgtacg atgtcaggct tccggttaat acaagttgat catcgtgttg
     5101 gtacgccgac atactctgag tatgagcgtt agcgacgtac cggagtgtag tgtatagtca
     5161 cgatgggagg tcacgtttta tgcgtgctgc cgttgaactt caaaaaatga agcttggagc
     5221 gacgcgtgtg ctaagtatgt aaaaaatttt tgggagcgtt tatcatgtta cgttggttag
     5281 caattatcat aatgactgct ttttttattt catcatgcga aaaagataaa aattcgtccg
     5341 atcctgtcaa tgatatggtt ttgataggtc aaggatacga tcaaggtgtt ttggtatcgg
     5401 tgtattccga agatacattg actaccggat ataatgcgat ctatacgcgt ttgcaggatt
     5461 cggcgagcgg agaaatactt gatgaaggac atattcacct gtcgccgttg atgcatatga
     5521 cggaaatgac acatgccgcg ccgtacggta atccggaaag cgaagaagcg gaagaaggat
     5581 tatttgaagg gtatgtcgta tttcaaatgc cgggcaatga tatggagtat tgggaattgg
     5641 aagtggaatt tcacgatcac cacagcggta aagaaggttc ggtgatgatc gaacccaagg
     5701 tcaaatcttc caatcgttgt aaagtcgtaa cgggaacgga tgcgaagtcg tacattcttt
     5761 catggaaaaa accgcgtgta ccggaagtcg gcttgaacac attggagctg atggtgcatg
     5821 agcgcgtatc catgatgtct tttcccgatg tgaccgatgc gacccttgcg atcgaaccga
     5881 cgatgccgtc tatgggacac ggatcgccta ataacgtaga tccggtgtat gttagcggtg
     5941 ccgtatatcg cggaaaagtc aattttacga tgaccggcga ttggcaagtg gatgtcagca
     6001 ttacccgcaa tgcggcaacc gttgtggaaa cgcattttga tgttaccgtt gattagtatg
     6061 tagagtccat gcggctttcg gagagcctcc cggaagccgc atgatttgaa agtaaaaagt
     6121 ttcgctaatt cattgagtgt aaatatgaaa cctctgttaa tgatcatcaa atactgtaca
     6181 gggttagttg ttttggtaac cctatccgta caagctcagg aacacgctac gatggtgcat
     6241 ggtcgtatcg tggatgaaga aacccgtgaa gcagtgcgtt atgcgcatat acggatggac
     6301 agcacggaag aaggtacgat agccgacagc gccggttact ttcatctccc gcgccatcac
     6361 ggcaaataca aactgcgcat ttcgtgcgtc gggtaccaaa ccgcatgggt gagcgtggat
     6421 cattttagtg aaaaaagaat catgcctgtt tttcttcgca gcaatgcgat tcgttttgaa
     6481 gaagaagtca tcgtttcagc cccgtggatc gagcaacagc aggaatcgta ttatcttgaa
     6541 aagaaactca acaccacgga cgacattctt gaaaaaacag acggcctttc tctg
//
LOCUS       DAOQNQ010000186         6570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_4509_length_6570_cov_4.734459, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000186 DAOQNQ010000000
VERSION     DAOQNQ010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4509_length_6570_cov_4.734459"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PK754_11060"
     CDS             complement(<1..841)
                     /locus_tag="PK754_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10088.1"
                     /translation="MILIAGVSIAVFIAALLISKKQKSQGDIILTAWMMIIAVQMLFY
                     HAYITDIIFRYPIFLAWERPLPLLHGVLLYWYVATMTDQRPSSKFLLYLHFIPTLFFY
                     LVLIPFFLRPVEEKIWVYRNEGAGYEILDAIRMGAIILSGTIYIIWSAILLSRHKRSI
                     LNRFSYEEKINLQWLRILTWGLGSVWLLIFISDEVLSVGVTIFVFMIGFFGIRQSRIF
                     KTTENNLEVPEKEKYAKSGLTKETAETLYAGLMRLMNEEAVYQKNDLSIYDLATRLNV
                     HPNY"
     gene            1025..2095
                     /locus_tag="PK754_11065"
     CDS             1025..2095
                     /locus_tag="PK754_11065"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNB10089.1"
                     /translation="MKTLLCFVILACGMMTSAQDLRFPDASPEAMIMQEVGISKVTIS
                     YSSPAVNGREIWGKLVPYGMEKSNPFGHGKPMPWRAGANENTTITLTHDAKIEGQTLK
                     AGTYSIHMIPEADQWVIIFNNNYTAWGSFFYEESEDALRVTVKPQMMADSQDRLSYGF
                     DNTDRNKTIAYLRWEKIKVPFTISFDTPQIVLNYIRQRLKNREAWDHGSLSQAAQFAL
                     RNNSLDEALSWTDRAINSGGGTSAFFLKSIILDKQGKKTEAEAVKKRAIDNATEADLN
                     TFGYQLLGQGKATEAVEVFRANAKKFPESWNVYDSLGDGLEAIGDKKGAIESFTKAQK
                     LVKDDANKRRIGDTLKRLQDSK"
     gene            complement(2181..5411)
                     /locus_tag="PK754_11070"
     CDS             complement(2181..5411)
                     /locus_tag="PK754_11070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB10090.1"
                     /translation="MHALYAQNEEGLIPTQYYSTSQFFAHNQNWAVTQDQQTGLIYAG
                     NNVGILEFDGATWRIIHTANRTMVRSLAYDPVGHRVLVGAQGEFGYLKPDSVGIMRYE
                     SLSAVLPEEARRFVNVWRIWVTNRGTFFQTNTEVFILQNETLRIIKPDQSSFHFSFLA
                     NGNVYVTLRNVGLQKFNGDKFETVPGGDRFHKIPIYAVLSYAPDTVLIATQEGLFLGL
                     NNGVIPFGETSKPFLKTNRVYNGLNLPDGRFALATLTGGVAILNRNGTIARVLDKSNG
                     LGDNAAKSIFLDRDKALWVSLEKGICRIEYFSPFTYFDDRVGLLSQFETFVRWKGKFY
                     IGTKDALYVLDRQPSSHIPYHFKTLYDVPEEHWSLLPMGDVMLDATFRNGIYEISSHG
                     RKRISPNSTAFLLRSRLDTHRVYAALWDGLSSVYLRNGIWVDEGRYAGITDEVRVIAE
                     DSDGSLWMDQKTGGCARMSRINDSIYVRRFSVKDGLPNLTINPYFFFGRMWFATDNGA
                     LYRYDEKASRFYPDTTLHHRLGIATSKMISPIGETSDGTLWFALGNDESMRFEPAYAV
                     PDGDKYRVTILAHGRLKIKLSPGNFFVEENAVWFYGHDNFYRVDRLRANGLSHDFKVN
                     IRKFTLNDSLLYGGALHSDLPTIVVPFGTSVIRIDYAATAFENQNDIHFKFKLDGFDE
                     EWSARTTEAFKEYTHLPEGKYTFRVKGYNVYEFESSEASIDFIVLPPWYRTWWSFVLY
                     FFVGAGIVYAGFRWRVRLLRLRNKELFVKVEERTRELASANHEIETQRDFLKNMNQQL
                     ESALKDLRETQTQLIQTEKMATVGQMVAGVMHEINNPLTFILPNIEYIEKSWKKLVTY
                     TERSKPHVAGAQDPELLAIIQDIERGLLIDETFGALDSMRRGSQRIKQVVVNLRAFAA
                     MDYLPKSETVVDAHIEQTVDLFFGTVKDLSFVKKLSYPTPIKVRIQEFNQSVVALLNN
                     AVQAIRDAEMQGTLERGKGVIEIRTQSHKGDANTLEFSISDNGIGIPEDILPRIFEPF
                     FTTRSVGQGRGLGLSEVYAVVHKHNGLITTQSKVGHGTTLTITLPA"
     gene            complement(5599..>6570)
                     /locus_tag="PK754_11075"
     CDS             complement(5599..>6570)
                     /locus_tag="PK754_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNB10091.1"
                     /translation="LSEKSYMGENLFSTPNPTVGAVFTYYLAEDIKTVKEKRRASEKE
                     KASKSESVFYPSMDSLKTEELQTEPTLIFTIKDDAGRIVRRLKAPAKKGMNRITWDFR
                     YPSPGPVTFNVPVSYNFYDVLEKGHVAMPGNYSVTMGKIEDGKYTELTPAQSFKAVAL
                     NASALSATDKNAFDSFAKSVSDWRRQISSADAYRGELAGKIKFMKAAALDAVEPGSEI
                     IEAVYTVERRLKEFNLKLNGDNIRDKYQFERAPSVSDRIGQIEFSIWNATAAPTETQK
                     QSLAVVAKQTPALMDELKSIDTEIKRIESMLERGKAPYTPGRMPEKK"
BASE COUNT         1971 a         1413 c         1349 g         1837 t
ORIGIN      
        1 ggtaattagg gtgaacatta agccgcgttg caaggtcata tatggagaga tcatttttct
       61 gatatacggc ttcttcattc atcagtctca tcaggcctgc gtatagggtc tcagctgttt
      121 ctttggtcaa gccggatttg gcgtattttt ctttttcggg tacttctaaa ttattttcag
      181 ttgttttaaa aatgcgtgat tgcctaatac caaaaaaacc gatcataaac acaaaaattg
      241 taacgccaac ggataacact tcatcggata taaaaatcag cagccaaact gagcctaggc
      301 cccatgtaag aatgcgtaac cactgaagat tgattttttc ttcatatgaa aaacggttta
      361 aaatgctccg tttgtgccgc gacaaaagaa tagctgacca gatgatgtaa atggtgcccg
      421 ataagataat cgcccccatc cgaattgcat ccaatatttc atatccggcg ccttcgttac
      481 gatagaccca aattttttct tcaaccggtc gcaaaaaaaa tggaataagt accagataga
      541 aaaacaaagt tggaataaaa tgaagataca aaagaaactt agaggatggc ctttgatcgg
      601 tcatcgtcgc aacgtaccaa tacaataata caccatgtaa caacggaagc ggtctttccc
      661 acgcaagaaa gataggatac ctgaaaataa tgtcggttat atacgcgtga taaaacagca
      721 tttgcactgc gataatcatc atccatgccg tgagaataat gtcgccttgt gatttttgct
      781 ttttagaaat caataaagcg gctataaata cggctatgct gacaccggca ataagaatca
      841 taaaaactaa agataatgga tatattggtt acacagacaa tatgaggtat taaaaaacca
      901 tgataaacag ttttttttat aagtatcaaa tctgaaaata tcgcggaaca aaatcatcat
      961 ctatacgtta ttaatatact tttcgttgtc ccatatctgt taattaatcc ttttaggaga
     1021 aaccatgaaa acgttgttat gttttgtgat cttggcttgc ggtatgatga cttcagcgca
     1081 ggatttgcga tttccagatg ccagtcctga ggcaatgatc atgcaagagg ttggcatttc
     1141 caaagttacg atttcctaca gtagtcctgc ggtcaatggt cgtgaaatct ggggtaaatt
     1201 agtaccctat gggatggaaa aaagcaatcc attcggacat ggaaaaccta tgccctggcg
     1261 tgccggtgct aacgaaaata cgacgatcac tctgacacat gacgctaaaa tagaaggtca
     1321 gactctcaag gccggtacgt atagcataca catgatccca gaagccgatc aatgggtaat
     1381 catttttaac aataactata cggcatgggg tagtttcttt tatgaagaat ctgaagacgc
     1441 actgcgtgtt accgtcaaac cgcagatgat ggcggattct caagatcgtc tcagttacgg
     1501 attcgataat accgatcgca ataaaaccat cgcctatctg cgctgggaaa aaatcaaagt
     1561 gccgtttaca atcagctttg atacacctca gatcgtactc aactatattc gccaacgtct
     1621 caaaaatcgc gaagcgtggg atcatggttc gttatcacag gccgcacagt ttgcgctgcg
     1681 caataattca cttgatgaag ccttgtcatg gactgatcgc gccatcaata gcggcggtgg
     1741 cacaagtgct ttttttctga aatcaattat tttggacaaa caaggtaaaa aaacagaagc
     1801 tgaagctgtt aagaaaagag ctatagataa cgccacagaa gcagacctca acacattcgg
     1861 gtatcaattg cttggtcaag gcaaagccac cgaagcggtt gaagttttcc gtgctaatgc
     1921 taaaaaattt ccagaatcat ggaatgtata cgacagtctt ggtgacgggc ttgaggctat
     1981 aggcgacaaa aagggggcta tagaaagttt tactaaagca caaaaattag tcaaagacga
     2041 tgctaataaa agacgtatcg gtgatacact taaaagatta caagacagta aatagtgctt
     2101 taagaattaa taaataccgc ttttactttt aagattatga ctgcgttgga atattttccg
     2161 acgcagtttt ttatttgaca tcaagccggc agagtgatgg taagagttgt accatgtccg
     2221 actttacttt gtgtagtaat aaggccgtta tgcttatgga caacagcata tacttccgaa
     2281 agcccgagtc ctctgccctg accgacggac cgtgttgtaa aaaatggttc aaaaattctc
     2341 ggaagaatat cttccggaat gccgatacca ttatcagaga tactaaactc aagggtgtta
     2401 gcatcgccct tatgtgattg ggttcgaatt tcgataacac cttttccccg ttcgagcgta
     2461 ccctgcattt ctgcatcacg gatggcttgt acagcattat ttagtaacgc aacgacggat
     2521 tgattgaact cctgaatgcg caccttaatt ggtgtcggat aagaaagctt tttaacaaat
     2581 gataaatcct ttacagtccc aaagaacaaa tcaacggtct gctcgatatg ggcatccaca
     2641 acggtttctg atttgggcaa gtagtccata gccgcgaaag cacgcaaatt gacgaccacc
     2701 tgtttgatac gctgactacc gcgccgcatg gaatctagtg ctccgaaggt ttcatcaata
     2761 agaagaccgc gttcgatgtc ttggataata gctaacagtt ccggatcttg tgctcctgcg
     2821 acatgcggtt tggaacgttc ggtatatgtt actaattttt tccagctttt ttcgatgtat
     2881 tcgatattgg gcaaaataaa agtcagcgga ttattaattt cgtgcattac gcccgcaacc
     2941 atttgcccga cggtagccat tttttcagtt tggatgagtt gtgtttgcgt ttcgcgaagg
     3001 tctttgagtg cgctttcaag ttgctggttc atgtttttaa gaaagtcgcg ttgcgtctca
     3061 atctcgtgat tagctgaagc cagttcgcgt gttctctctt ctactttgac gaataactct
     3121 ttatttcgca aacgcaacaa acgcacacgc caacggaaac cagcgtacac tatccctgct
     3181 ccgacaaaaa aatacagaac aaaactccac catgtcctat accatggcgg taaaacaata
     3241 aaatctatgg acgcttccga cgattcaaat tcatagacat tgtaaccttt gacacgaaat
     3301 gtgtatttgc cttcaggcaa atgtgtgtat tctttgaagg cttctgtagt gcgtgcagac
     3361 cattcttcgt caaacccatc aagtttaaat ttgaaatgta tatcattttg attttcaaac
     3421 gcggtggctg cataatcaat ccgaatgacg gacgttccga acggtacgac gatcgtaggt
     3481 aaatcgctat gaagagcgcc gccatataat aacgaatcgt tgagagtaaa tttacggata
     3541 tttactttga aatcatgtga aagtccgttg gcgcgtaaac gatctacacg ataaaaattg
     3601 tcatgtccat aaaaccagac ggcattttct tcaacaaaga aattacccgg cgaaagttta
     3661 atctttaatc gtccatgtgc caaaattgta acacggtatt tatcgccgtc aggcacggca
     3721 taggcaggtt caaaacgcat tgattcgtca ttacccaaag caaaccacaa tgtcccatcg
     3781 gatgtttccc caatcggaga aatcatttta gatgttgcga ttcctaaccg atgatgcaac
     3841 gttgtatcag gataaaatcg agatgctttt tcatcatatc tgtagagtgc gccattatcg
     3901 gtagcgaacc acattcgtcc aaagaaaaaa taaggattaa tagtcaagtt aggcaaaccg
     3961 tctttgactg aaaaccgccg cacgtatata ctatcgttga tgcgggacat acgtgcgcaa
     4021 ccgccggttt tttgatccat ccataatgaa ccgtcgctgt cttcggcgat gacgcgcact
     4081 tcgtcggtaa tacccgcata acgaccttca tccacccaga ttccattacg caaatatacc
     4141 gacgacaaac catcccagag tgcggcataa acacgatgcg tatccaatcg tgatcgcagt
     4201 aaaaacgccg tactattggg tgaaatacgt tttctgccgt gtgatgaaat ttcataaata
     4261 ccatttcgga atgtcgcatc caacataaca tcacccatcg gcagtaagga ccaatgctcc
     4321 tcgggtacat cgtataatgt tttgaaatgg tatgggatat gtgaacttgg ctggcgatcc
     4381 aacacataca atgcgtcctt agtgcctata taaaatttcc ccttccaccg tacgaaagtt
     4441 tcaaactgac ttaataatcc gacgcgatca tcgaaataag tgaacggtga aaaatattct
     4501 attcggcaaa tccccttttc caatgaaacc caaagagctt tgtcacggtc aagaaatatg
     4561 ctttttgctg cattgtcacc gagcccgtta cttttgtcca gtacgcgtgc gattgtaccg
     4621 ttacgattta aaatcgctac accgcccgtc aaggtagcca gtgcgaaacg tccatcgggc
     4681 aagttcaaac cattgtagac gcggtttgtt ttgagaaacg gtttgctggt ttcgccaaac
     4741 ggaatcactc cgttatttaa tccaagaaac aatccttctt gtgttgctat caataccgtg
     4801 tcaggcgcat acgatagcac agcataaata ggaattttat gaaaacgatc tccacctgga
     4861 acggtttcga atttatcacc attaaacttt tgtaatccta cattgcgcaa agtcacatat
     4921 acattgccgt tagccaaaaa agaaaaatga aatgaagact gatccggttt tatgattcgc
     4981 aatgtttcgt tttgaagtat gaaaacttcc gtattggttt gaaagaaagt acctctattt
     5041 gtaacccaaa tgcgccatac gttgacaaaa cgacgcgctt cttccggcaa gacggcagac
     5101 aatgattcgt aacgcataat accaactgaa tctggcttga gataaccgaa ttcgccctgt
     5161 gctcccacta aaacacgatg accgaccgga tcataagcca gcgaacgtac catcgttcgg
     5221 ttcgccgtgt gaatgatgcg ccacgtggct ccgtcaaact ccaaaatgcc aacattattt
     5281 cccgcgtaga tcaatccggt ttgttgatct tgtgtcacgg cccaattttg attatgcgca
     5341 aaaaattgac tggttgaata atattgggta ggaattaatc cttcttcatt ctgtgcataa
     5401 agtgcgtgca aaataaaaaa gtataaagaa aatattgaaa tcctgagggg catcatgaag
     5461 atactattta gtagacggga ttagaaaacc aaaaaagggt actaaaaatc aaagcctgaa
     5521 tcaaacaaaa aaaaactgcc tgagaatttt atattcgtca ggcagtctgt agactataaa
     5581 aaaactaaaa attatctttt attttttctc cggcatacgt cctggagtat aaggcgcctt
     5641 accccgctca agcatggatt cgatacgttt gatctcggtg tctatggatt tcaattcatc
     5701 cataagcgcc ggtgtttgtt tggctactac agccaaagat tgtttttgag tttcagtcgg
     5761 tgccgctgtc gcattccata tgctaaactc aatctgaccg atacgatcgc tgacggacgg
     5821 agcgcgttca aactgatatt tgtcacgaat attatcacca ttgagtttga ggttaaattc
     5881 ctttaggcgg cgttcaacag tgtaaacggc ttcaatgatt tctgatccgg gttccacggc
     5941 atccaatgca gcggctttca taaacttgat tttacctgcc aattcaccgc gatacgcatc
     6001 ggcggaactg atttgacggc gccaatccga gacagatttc gcaaacgaat cgaatgcgtt
     6061 tttatccgtg gcagacaatg cggatgcatt caatgctaca gctttaaaag attgcgccgg
     6121 agttaattcc gtatattttc catcttcgat tttccccatc gtgacgctat aatttccagg
     6181 catggcgacg tggccttttt caagcacgtc atagaaatta tatgaaaccg ggacattaaa
     6241 agtaaccggg cccggcgatg gataacggaa atcccaagtg atacgattca tacctttttt
     6301 ggccggcgct ttgaggcgtc gtactatacg tccggcatcg tctttgatcg taaaaattaa
     6361 tgtcggttca gtttgcagtt cttccgtttt gagtgaatcc atagacggat aaaataccga
     6421 ttcacttttg gaagccttct ctttttctga agcgcggcgt ttttctttga ccgttttgat
     6481 gtcctcggcc agataatacg taaacacagc gccgactgtc ggattagggg tactaaatag
     6541 attttcaccc atgtatgatt tttcgctaag
//
LOCUS       DAOQNQ010000187         6561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_4525_length_6561_cov_5.809099, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000187 DAOQNQ010000000
VERSION     DAOQNQ010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6561
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4525_length_6561_cov_5.809099"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2652)
                     /locus_tag="PK754_11080"
     CDS             complement(<1..>2652)
                     /locus_tag="PK754_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10092.1"
                     /translation="YFGFNNPSIQSDTVFSYKALTIYGQATNVGGKRDGSYINPNVFA
                     SYMSYGEVYSDSNVIMGNMFGLQHDGQTPAPFLAAGLTIYGDDNLIGHHTRPGRNVFG
                     SGENASALLLIQAHRNTIIGNYFGTDSTGLSAGLLNRGIQLVSGSSENIIGGATVGER
                     NIFSTTQVDIYVQDGTANHILGNYFGLNADGSATLVRGGIGVDNASPGLIVGDGSDDG
                     RNYFVNKSTAINTYTSSRATEVQNNVFGLMPDGVTVEPNNTALVLSGNASVIDDNIFA
                     GTNAGRAIQVFADSCFLSGNIIGVDVLGNGPKGFNIALEINGENNRIGSRTSGEGNSI
                     SWSTQLISIGSGANQNHIEGNAIGLGIENQIRGGGHGISVNQARGNTIAYNKIGSIVN
                     AINLSNTYNTHIHHNAIGVADSLGSIQRGNSGVGISITSNTSGTLIEHNTIAYNNISG
                     ISIVDAESDSNRWYWNRIYENKSGITRPSNSKNSVTPPHIMGGIANVGVWGKAQPGSR
                     VELFLDEPGKIQASDIIGGTYADAQGTWAISVPGLSEGQTLAATQDSAFNSSLWSTRY
                     IPSSAGNILERDHGYRAVISLGSIKAFDSISVPLRFYADDGSVISQIAMHSGDEFSWD
                     GKNADTLDHSYPDTLIGWVKFKPESEGVFSDTLRVVYDSRVFALTLEGVATEPSVLTS
                     EELQYSFPNTRVTYVSNRQVELYAPKAPLNVTAIRLARAGTSGYSFDGSSLPYSIDSD
                     TLRVGVHFTPSAIGVSLDTLIFSHDGYQRELRIPLQGLGLENQAPVVHTGLLRSTVLK
                     DRIEFYAVSDEPLSSVSLKINNTTVPLQIVPAHTRMYRAEYEITADGELAFEVTANDT
                     ILNQAIALKTYVIADLGSGSTSVLRSGQ"
     assembly_gap    2653..2752
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2766..3653)
                     /locus_tag="PK754_11085"
     CDS             complement(2766..3653)
                     /locus_tag="PK754_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB10093.1"
                     /translation="MFWNWFLAIGLFVSLSNILFSQAQDSIRIRTVPVAGKVWMMDCE
                     NGFGGGNVAAMAGDEGVLLVDAMYASMTEKIKKAAFTLSNLPIRWVVNSHFHRDHIEG
                     NKNLRTSAVIIGQENIAKRLRSKHTQTTPTLDMMPSVFFSDSLTLTVNGEDIRIIHMP
                     GSHTSADVVVYFTQSNVLHLGDLFFHGMFPAVYTDGGGNIHQLIASLDKIEKMFPVDV
                     KIIPGHGPLATMPALHEYIIMLKETVTAAQEGLRNKQTSDQLLKTSVFQKYNYLGDGG
                     AQTTEQYINMLFKLLSESK"
     gene            complement(3901..4065)
                     /locus_tag="PK754_11090"
                     /pseudo
     CDS             complement(3901..4065)
                     /locus_tag="PK754_11090"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(4645..5688)
                     /locus_tag="PK754_11095"
     CDS             complement(4645..5688)
                     /locus_tag="PK754_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10094.1"
                     /translation="MNRSKSLLIILIAALVLIPSCDNVESGTFSGIYFGRPPRKLRDG
                     QGYFEMWLGFPASSGGKEWVSILKFDRVESTNKPFLNDKAAILKRPNRNINDAVSAAI
                     SIELENDIDPSVPNRIFMSGEFTDGIASLELSGEDALNLDLSDPAVISGKFVLGTPSD
                     AINNNDDSGVWFVDAPFGQQQNPGLSLPVLPIGFKYEAWIATQSETVCISTGQFKRAD
                     SLDYNGKGPNGGGELTPNVPGEDLVFNQFNEDVQGRTVDASLQEAYLDTFGFPIHIKK
                     TIQKNPLTNAKDYWDVVVSVEPDPDNDPEEPFAIFPLVKFGPIFESVNTVIPLTNFTG
                     GVSQYIVIDLSKM"
     gene            complement(5713..>6561)
                     /locus_tag="PK754_11100"
     CDS             complement(5713..>6561)
                     /locus_tag="PK754_11100"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HNB10095.1"
                     /translation="PTIQFRTGLKDRVPETVPPEAQFVNFRVAPPMWGRGFIQEIPAE
                     TIVANADPNDANDDGISGRANYIVMSFVDSPTHPELGRHGLKAQMSRILDFADNAFLH
                     DLGMTSPMRPYELVNPNARDAKDKKSGVDISHEDVEKVDFFIRTSDFPPILQETEQIQ
                     IGKNLFNEIGCNKCHIPSMTTGTSEISALSNKTVFFYSDFLLHDMGQTLSDGVLDGDA
                     KPQEFKTPTLRGLRFFKDFLHDGRAKNVDAAIRMHGGEATTVRNNFVGLSQEERDAII
                     AFLNSI"
BASE COUNT         1850 a         1467 c         1274 g         1870 t
ORIGIN      
        1 ttgaccggaa cgaagtacac ttgtactgcc ggaacccaga tcggctatga catatgtttt
       61 gagagcaatg gcttgattga ggatcgtatc attggccgtc acttcaaaag ccaattcccc
      121 gtcggctgtg atttcgtatt cagctcgata catacgcgta tgcgccggta cgatctgtag
      181 cgggactgtc gtgttattga tttttagcga aaccgacgat agtggttcat ccgatacagc
      241 gtaaaactca atccgatctt ttagaaccgt acttcgcaaa agaccggtgt gcaccaccgg
      301 tgcctgattt tctaaaccta atccctgcaa aggaattcgc agttcgcgct ggtatccgtc
      361 gtgactaaaa atgagcgtat ccagagatac acctatcgcc gacggtgtaa aatgtacacc
      421 cacgcgcaac gtatcgctat cgatcgaata cggtaaactg cttccatcaa aactataacc
      481 ggatgttccg gcacgcgcta atcgaatagc cgttacattt agaggcgctt tgggagcata
      541 cagttctacc tgacgattcg agacataggt tacgcgtgta ttgggaaaac tatattgaag
      601 ttcttccgat gtaagtaccg aaggttctgt cgcgacgccc tccaaggtca acgcaaatac
      661 tcggctatcg tacacgacac gtagtgtgtc cgaaaaaaca ccttcggatt caggtttaaa
      721 tttgacccat ccgatcaacg tatcggggta actatgatcc agcgtatctg cattttttcc
      781 gtcccaacta aactcatcac cggaatgcat cgcaatttgc gaaataacgg aaccgtcatc
      841 cgcataaaac cgcaatggaa cggaaatact atcaaatgct ttgatgcttc cgagagaaat
      901 tacagctctg tagccatgat cacgctccag tatattaccc gccgatgaag ggatatagcg
      961 cgttgaccat agggacgaat tgaacgcgct gtcttgtgtg gccgcaagcg tttgaccttc
     1021 cgataatccc ggaacagaaa tagcccacgt gccttgtgca tccgcgtacg taccgccgat
     1081 aatatctgaa gcttgtattt ttccgggctc atctaaaaat agctcaacac gggaacccgg
     1141 ttgtgctttt ccccacacgc ctacattcgc gatgccgccc atgatgtgag gaggcgttac
     1201 gctgttcttt gaatttgaag gccgtgtaat accgctttta ttttcataaa tacgattcca
     1261 ataccaacga ttactgtccg attctgcatc gacaatactg atacctgaaa tattattata
     1321 ggcgatggta ttatgttcta tcaaagtacc ggatgtattc gatgtgatgc taataccaac
     1381 gccgctattc ccgcgctgaa tactacccaa tgaatcggct acgccgatag cattatggtg
     1441 aatatgtgtg ttatacgtat ttgataaatt gatcgcatta actatcgaac cgattttatt
     1501 ataagcgatg gtgtttccgc gagcctgatt aacagatatg ccgtgcccac cgccgcgtat
     1561 ttgattttcg atgccaagac cgatagcatt gccttcgata tgattctgat tcgccccgct
     1621 cccgatagaa atcaattgcg tggaccagga gatactgttt ccttcgcctg atgtgcgcga
     1681 tccaatgcga ttattttctc cgttgatttc taacgctata ttaaatcctt tgggaccgtt
     1741 acccaataca tcaaccccta ttatgtttcc tgataaaaaa catgaatcgg caaacacctg
     1801 gattgcacgc ccggcattgg ttcctgcgaa aatattatcg tctatcacac tggcatttcc
     1861 actcaaaacc agcgccgtat tgtttggctc taccgtcaca ccatccggca taagcccgaa
     1921 aacgttgttc tgcacttcgg tagcgcggga agatgtatac gtgttgattg ctgtactttt
     1981 attgacaaaa taattacgtc cgtcatcgct gccgtcgcct acgatcaacc cgggtgatgc
     2041 attgtcaaca ccaattccgc cacgtactaa cgtggcgctt ccgtccgcat tcaaaccaaa
     2101 ataattacca agaatatgat tggcggtgcc gtcctgaaca taaatatcca cttgcgttgt
     2161 ggaaaaaata ttcctttctc cgaccgtcgc tccgccaata atattttctg aactccccga
     2221 caccaactga attccccgat taagcagacc tgcgctgaga cccgtgctat ccgtgccaaa
     2281 ataattaccg atgatggtat tgcgatgcgc ttgtataaga agtaatgccg acgcgttttc
     2341 accgctgcca aatacattac gtcccggtcg tgtgtggtgg ccaatcaggt tatcgtcgcc
     2401 ataaatcgtc aagccggcgg ccaaaaatgg cgccggagtt tggccgtcat gctgcaatcc
     2461 gaacatatta cccatgataa cattactgtc cgaatacact tcaccgtaac tcatgtagct
     2521 tgcaaaaaca ttggggttga tatacgaacc atcgcgtttc ccaccaacat ttgtcgcttg
     2581 cccataaata gtaagcgcct tatagctgaa taccgtatcg gattgaatgc tcggattgtt
     2641 gaatccaaaa tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctcgcgat
     2761 gagaattatt ttgattctga aagtagtttg aatagcatat ttatatactg ctccgtagtc
     2821 tgagcgcctc cgtcaccgag gtaattatat ttttgaaata cggaggtctt caaaagttga
     2881 tccgaagttt gtttattccg aaggccttct tgcgcagctg ttacggtctc tttgagcata
     2941 atgatatatt catgcagtgc tggcatcgta gccaatggcc catgtcccgg gatgattttg
     3001 acatccacag gaaacatctt ttctattttg tccagcgatg caatcaattg atgaatattt
     3061 ccaccgccgt cagtgtacac ggccggaaac ataccatgaa aaaataaatc accgagatga
     3121 agtacattgg attgcgtaaa atacaccacg acatcggccg acgtgtgact tccgggcatg
     3181 tggataatgc gaatatcttc cccattgacg gtgagagtga gactatctga aaaaaatacg
     3241 ctcggcatca tatccagagt cggtgtagtt tgtgtgtgct tactacgcaa acgcttagct
     3301 atattttctt ggcctataat aaccgcagat gtacgcagat ttttgtttcc ttcgatgtgg
     3361 tcacgatgaa aatgcgaatt gaccacccac cggatgggta aattggaaag tgtaaaggcc
     3421 gctttcttta ttttttcagt catagaggcg tacatcgcat ccaccaacaa gacaccttca
     3481 tcaccggcca tcgcagctac attaccaccg ccaaatccgt tttcacaatc catcatccac
     3541 actttaccgg cgacgggtac ggtgcgaata cgaatactat cctgcgcttg cgagaaaagg
     3601 atattactga gggacacaaa tagtccgatc gctaaaaacc aattccaaaa cattttgaat
     3661 tccttccaca tgagcacatg agtattatta cacaatccta ccgaatcaaa gattcacgat
     3721 gctgtttaac tattaataat aatagatcaa ctctaagtcc gagttaatcc agattaactc
     3781 aatcaaattt tgatagtgaa aatttacaga taagacgaga tgcgcgctaa cggtagtggg
     3841 tcaattatta gcggttttgt tgcaacttat gttgcggaga ttgtttttaa agcttttaga
     3901 tcagatcgta tcggaggaat ttatatcaac ttcatcgcga aaagctttgg taaagtgtga
     3961 caaactggta taaccgacat cataggcgac ctgcgtcaca ttaccggctt gtgattccaa
     4021 caaacttttg gctttccgta aacgcagttt tcgtatgagt ttggatgggt ggtggttgta
     4081 agagaattag cttgataagc tagaccggct tattgtcaat tgagttgtaa ttccgtagca
     4141 tcaatgatta tgaatcagat taataaaatt tttcccattg ataaaaaaac gaatttagaa
     4201 acaaaggtct aaaggatatt tccattttct ttatccatgt catcataaat actatgccgc
     4261 tacgagcctg aaaatcaacg cgtgcagaat atttcctgat atgcagaaaa acttacgagg
     4321 tcacccgatt aaaagctttt gtattgcttg agcataccaa cctctacggc tgctatagat
     4381 atcataaccc tagcgccatc taagatttca aatagctttt gataacacaa aacacgtcta
     4441 tcgtacaata tagaaaaatt aaacttattg aaatctgtac cttaatcagc aattcgaccg
     4501 gctagtgatc aaaatcaatt gagccaggct ttctagtggt tttaactttc atatcaatgt
     4561 aaatattgac tgcgtctaac ttccccacct aactatatga atttgacaaa tatgctttca
     4621 tttaatttat caaatatggg catttcacat ttttgataaa tcaataacaa tgtattgaga
     4681 cacacctccg gtaaaattag tcaaaggaat tactgtattt acggattcaa aaattggacc
     4741 aaatttaaca agaggaaaaa tcgcaaacgg ttcttcaggg tcattatctg gatccggctc
     4801 aacagacact actacatccc aataatcttt agcgttagtc aaaggatttt tctgaatggt
     4861 ttttttaata tgaattggaa atccaaatgt atctagatat gcttcttgta acgaagcatc
     4921 cacagttcga ccctgtacgt cttcattgaa ttgattaaat actagatcct ctcccggaac
     4981 atttggagtc aattcaccgc ctccattagg tcccttaccg ttatagtcca aactgtccgc
     5041 acgtttgaat tgaccggttg aaatacatac tgtttcggat tgcgtagcga tccaagcctc
     5101 atatttaaat ccgatgggta gtacaggaag cgataaaccc ggattttgtt gttgaccaaa
     5161 tggtgcatca acaaaccaga ccccgctgtc gtcattatta tttattgcat cggaaggagt
     5221 tcctagtacg aatttcccgc taatgacggc tggatcactc agatccagat ttaaagcgtc
     5281 ttcaccgctg agttccaaag atgcaatacc atcggtgaat tctccgctca taaatattct
     5341 gttgggcaca gaaggatcta tatcattttc aagttcaata gaaatggctg ctgaaactgc
     5401 atcgttgata tttcgattag gtctttttag tattgctgct ttatcgttta aaaatggctt
     5461 attagtactc tccactcgat caaactttaa aatacttacc cattctttac caccgcttga
     5521 tgcaggaaaa cctagccaca tttcgaaata tccttgccca tctcgtaatt ttcgcggtgg
     5581 tctaccaaaa taaataccac tgaaagttcc gctttctaca ttatcgcatg aaggaattaa
     5641 aactaatgcc gcaattaaaa ttattagcaa tgattttgaa cgattcatat ttaaagcaag
     5701 gtcctgtatt agttaaattg aatttaaaaa tgctatgatt gcatcacgct cttcttgtga
     5761 caatcctaca aaattattgc gaacggtcgt tgcctcaccc ccatgcattc gtatagctgc
     5821 atctacattt ttggcacgtc catcgtgaag gaagtcttta aagaaacgca accccctaag
     5881 ggtaggtgtt ttgaattctt gtggtttagc gtctccatct aatacaccat ccgagagagt
     5941 ttggcccata tcatgaagca gaaagtcgga ataaaaaaac accgttttat tacttaaagc
     6001 agaaatttca gatgtaccgg ttgtcataga aggaatatgg catttgttgc aaccgatctc
     6061 attaaacaaa tttttaccaa tttgaatctg ttccgtttct tgtaaaatgg ggggaaaatc
     6121 gcttgtacgt ataaaaaaat ctactttttc tacatcttca tgactaatgt ctacacctga
     6181 ttttttatct tttgcatcgc gcgcattagg attaactaat tcatacggtc gcataggtga
     6241 cgtcattccg agatcatgca agaaagcatt gtcggcaaaa tctaaaattc gcgacatttg
     6301 cgcttttaag ccgtgacgcc caagttcggg gtgtgtcggg gaatcaacaa aactcataac
     6361 aatgtaattc gctcgtcctg aaataccatc atcattagca tcattaggat cagcgttggc
     6421 gacaatcgtt tcagccggta tttcttgaat gaatcctctt ccccacatgg gaggagcgac
     6481 tcgaaaattt acaaattgcg cttccggggg gaccgtctcc ggtacgcgat cttttaatcc
     6541 ggttcgaaat tgtatagtcg g
//
LOCUS       DAOQNQ010000188         6561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_4526_length_6561_cov_5.367661, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000188 DAOQNQ010000000
VERSION     DAOQNQ010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6561
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4526_length_6561_cov_5.367661"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PK754_11105"
     CDS             complement(<1..369)
                     /locus_tag="PK754_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing system transmembrane
                     subunit LolC"
                     /protein_id="HNB10096.1"
                     /translation="MNLFSYPSFIARRYLWAKRKNGFISIISIISMAGVALGTAALII
                     TLCIISGFENQLITKFINFDAHIRLKTFDGTEPIGDTNMIEEQLQGYKSIIGFSPYIE
                     KEAMIRSSQITDGVIVKGINE"
     gene            complement(366..1103)
                     /locus_tag="PK754_11110"
     CDS             complement(366..1103)
                     /locus_tag="PK754_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10097.1"
                     /translation="MEKRLPEVKENDINELIRLLKEEEEWYKKKLKYDDINEKIFSVS
                     ETVKRIIDNTETECKLIEVRLEDARKLTAYLPDLEREHETLTREFHRLTKIREKQDQI
                     TKQGLKDRIELELLQQQIDQLIVTKSDWLIRLDQTQKELTRLREEYLLIENDLKLFPD
                     VEKRLIANEFFIEQAHKAEKDVRILSDEMQKLKHKLTYKTMIRRESGLLDALKTELQS
                     IQYDPQKHFECREALFHLTDRIAQGES"
     gene            1197..4397
                     /locus_tag="PK754_11115"
     CDS             1197..4397
                     /locus_tag="PK754_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNB10098.1"
                     /translation="MNNPESLTIYRASAGSGKTFVLVRDYLKITLGKPEQFRHILAIT
                     FTNKAAQEMKLRIIEALRAFSSDHTLSTSDHHLFEYLRNTLGFSDEQLRTAAQKLLIT
                     ILHEYSDFAVSTIDSFMQRILKAFATDTGLPISFSMELDSMRVAKSILERLYNAIDQN
                     EELSDFLIQWAQERSSEGKTHNLDSELLRFIPIIFEENHAAPLERIKTHELIDFVRLR
                     NTIRHQMSSITLEWQNKLKQIVDEIVSLGLTDEDFYRKTKGVLSFIKKSFENPFNIEP
                     NQYVVAALTDRSLVHPDSSKKDILKTIEHKILQLIDQYLTVQKKITSYDLINKALPQL
                     TLMRTFTELYDQFRDEELTIHISEVNHHINKIVRSEPVPYIYEFISSKYSHFFLDEFQ
                     DTSAMQWQNLVPLIHESLSYGTYRHNLIVGDVKQSIYRWRGGRAEILANLPYLTEKIK
                     LELEEGDAIESKFLETYKIVNLNTNYRSKEVIVNFNNRFFEFLKTSHWFKFNHYYDQC
                     AQETASSQTGGYVKIEFLTQNDSNSELYYEAALKTKKIIQTCCEELKYSPHDIAILVR
                     SQHEAIAISDILSEYQIPITTEESLLLRNAPKVRYLIAFLHWVHNPKNKNHLATWLTL
                     AAQLTHRHQDFFQNIFLIGHSSQSIPEIFDFAINELKLDTSIRKINEQPLHEALLTLI
                     YASHHIIGATDQFVECFVKIALEYAQTQSLLLNEFLNYWEENNETLALRNDGLRSGVR
                     IMTVHKSKGLEFPVVIIPIADWSLSSTGKNKLWVNTDPERFFGMDTLLIPEVKKIQNS
                     DFYEFYKNEIELLQLDAINLLYVAMTRSRDHLYIMCPTPTANSDKENVRNVGEIISQF
                     VIHESSQLNTYEAGSSAYSNQTQHVKEQEKNLVYTQTETVKEKPWRSWIQLQSRFDNP
                     SDLDTLWYGDKNTRWGNLIHFVLVQMSRSEDLMKAIEHFKKISFVNEEEAHHIHTVLT
                     RFFENKSIVKLFDATAAIIRESEFFDTEGQLRRFDRMQIENDRITIIDFKTGQKKDTH
                     VTQMQSYMKPFVQIHHQKVQGALLYIDPPEIVEVHL"
     gene            4394..>6561
                     /locus_tag="PK754_11120"
     CDS             4394..>6561
                     /locus_tag="PK754_11120"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNB10099.1"
                     /translation="MIAFLDDIATYLLKDSPTSLNDRCVVFQNQRAKLYFKKYIGQRA
                     QPGLILPEMLTIEELAERLSGKRITDNFDLLIELYLCHQKLNQPETFIKFYDLGLKII
                     EDFSDMDMYAVDPAKLFINLDYLKSTEFWDKNDEDLTVLQLRQKNFWKYLFTLHKEFK
                     RVLDEKGKAYAGMAYREAVDQIRSQNYTLPWSKVFFAGINALSLCEEEIIDHLLRRKQ
                     AEIFWDADVFYINQKHHEAGNFIRRYIKKWPESDKHWIGDRLSRDNKTIRITRASQNS
                     AQARAAVVALKESVKNKAGSIAVVLGDESLLIPVLEELPESLTKSTRINVSMGYPLSM
                     TTIHSFMLCVLQLHADALSNQTKRKEYTFLHQYVIQFFSLPLVRNLIKDKVYSIHQAI
                     IRQNSTKLSLEFIQSLTNLRSLKLVFNACCDISDMLKNIDSLIDEIKNELFINRMYEE
                     LEALYLWKGKWTIICDTYQRHTSLHNPDLSENLETIIRLIQDAVINVRIPFDSPAVNS
                     DLTQRIEIMGMLETRALDFEHVIILSVNEGVFPAPRSTHSLIPFTARAESGMPNYTEK
                     ESIFAYNFYRLLQRASTIDLIYDAQINQNTKGEPSRFIQQLRYELAKTSPNITIIENE
                     LPPLPVIKSSIEPLLIQKTPAIIDAITDVLIKGLSPTAINSYNRCSLQFYLSHIAKFD
                     ESDDIKSELQSDDLGNIVHHVFQHLFTPYIGKSIAAKDISS"
BASE COUNT         2148 a         1274 c         1136 g         2003 t
ORIGIN      
        1 ttcgttaatt cccttgacaa taacaccgtc tgtaatttga gacgaacgaa tcatagcctc
       61 tttttcgata taaggggaaa atccgatgat ggatttatat ccctgaagtt gttcctctat
      121 catattcgta tcacctatcg gttctgtgcc atcaaatgtc ttcaaacgaa tatgtgcgtc
      181 aaaattaata aatttagtaa tcaattgatt ttcaaaaccg gatatgatgc acagggttat
      241 gataagcgcg gctgtgccta aggccacgcc ggccatggaa ataatggaaa taatggaaat
      301 gaaaccattt ttacgtttgg cccacaaata acggcgagct ataaaagaag gatacgaaaa
      361 caaattcatg attccccttg agcaatacga tcggtgagat gaaacaatgc ctcacgacac
      421 tcaaagtgct tttgcgggtc atattgtatg ctttgaagtt ctgttttgag cgcatccagt
      481 agtccggatt cacgtcgaat cattgtttta taagtcaatt tatgttttag tttttgcatt
      541 tcgtctgaca aaatcctcac gtctttttcg gctttatgtg cctgttctat aaaaaattca
      601 ttcgcaatta aacgtttttc tacatcggga aataatttta aatcattttc tatcaaaaga
      661 tattcttccc ttagacgcgt taattccttt tgggtttgat ccaatcgtat aagccaatca
      721 gacttcgtaa cgatcaattg gtctatttgt tgttgtaaca attcgagttc gatgcgatcc
      781 ttaagacctt gtttggtaat ctgatcctgc ttttcacgaa ttttagtcaa ccgatggaat
      841 tcacgcgtca gagtttcatg ctctcgctcc agatccggaa gataagcggt taacttgcgc
      901 gcgtcttcca aacgaacttc aattaattta cattccgtct ccgtattatc aatgatgcgt
      961 ttaacggttt cagaaacaga aaagattttt tcattaatat catcgtattt cagtttcttt
     1021 ttataccact cttcttcttc ttttagcaaa cgaattaatt cgttaatatc gttttctttt
     1081 acttcgggta accttttttc cataatatag tttaccaatt ttacacaata aaacaaagtt
     1141 tttgattata tcagcagcct tatccatatt gattaatcac atttacaata ctagaaatga
     1201 ataaccctga aagtctaact atctatcgtg cttcggcagg atccggcaaa acattcgttt
     1261 tggtgcgcga ttatcttaaa atcactctgg gtaaacctga gcaatttcgg catattctcg
     1321 ctattacgtt tactaataaa gccgcccaag aaatgaaact tcggattatt gaggcgctgc
     1381 gagctttttc ttccgaccat acattatcaa catcagacca tcatttattt gaatatctac
     1441 gtaatacttt aggtttttca gatgaacaat tgagaactgc cgctcaaaaa cttttgataa
     1501 ccattctgca tgaatatagc gattttgctg tttctaccat tgacagtttt atgcagcgta
     1561 tattgaaggc ctttgctact gatacagggc ttccgatcag tttttcgatg gagctcgaca
     1621 gtatgagggt tgctaagtct attttagagc gattatataa tgcgattgac cagaacgaag
     1681 aattatccga tttcctcatc caatgggcgc aagaacggtc cagcgaaggt aaaacacata
     1741 atttagacag cgaattattg cgatttatcc ccataatttt tgaagaaaat catgctgctc
     1801 ctttagaacg aatcaaaacg cacgaattga ttgatttcgt gcgattacgc aatacaatac
     1861 gccatcaaat gtcaagcatt actctggaat ggcagaataa actaaaacaa atagttgatg
     1921 aaattgtgtc gttaggatta actgatgaag atttttatag aaaaactaaa ggcgtacttt
     1981 cgttcattaa aaaaagcttt gaaaatccat ttaacataga gcctaatcaa tacgtcgttg
     2041 cggccttaac cgatcgttcg ttggtacatc ctgattcttc caaaaaagac attttaaaaa
     2101 ctattgaaca taaaatatta caattaattg accagtactt gacggttcaa aaaaaaatca
     2161 cttcttatga tttgataaat aaagcgttac cgcaactaac tttaatgcgg acattcaccg
     2221 aactttacga tcaattccgt gatgaagaac ttacgataca tatttcagaa gtaaatcatc
     2281 atataaacaa aatcgttcgt agtgagccgg ttccgtacat atatgaattt atttcgtcta
     2341 aatatagtca cttttttttg gacgaatttc aagatacctc tgctatgcaa tggcaaaact
     2401 tagtgccgtt gattcatgaa agcctctctt acggcacgta tcgacacaac ttgatcgtcg
     2461 gtgacgtcaa acaatccatc taccgctggc gtggtggccg cgctgagata ttagcaaatc
     2521 taccctatct taccgaaaaa attaaactcg aacttgaaga aggcgacgcc attgagtcta
     2581 aatttttaga aacgtataaa atcgtcaacc ttaatacaaa ttatcgaagt aaggaagtaa
     2641 tagtcaattt taacaatcgt ttttttgaat tcttaaaaac atcgcactgg ttcaaattca
     2701 atcattacta tgatcaatgt gcgcaggaaa ccgcatcttc gcaaaccggg ggttatgtca
     2761 aaattgaatt tttgactcaa aatgacagta atagcgaact gtactacgaa gccgctctta
     2821 aaactaaaaa aataatacaa acgtgttgtg aagagttaaa atattctcct cacgacattg
     2881 ccattctcgt tcgatctcag cacgaagcta ttgcgatctc tgatatttta tcagaatacc
     2941 aaattcccat aacaactgaa gaatccctat tacttagaaa cgctcccaaa gttcgatact
     3001 taattgcatt tttgcattgg gtccacaatc caaaaaacaa aaaccatctt gccacttggc
     3061 tgactttggc agcgcagtta acacatagac atcaagactt ttttcaaaac atatttttaa
     3121 tcgggcactc ttcccaatct attcctgaaa tttttgattt tgctatcaat gaattaaaat
     3181 tggatacatc aataaggaaa atcaacgaac aaccattaca cgaggcgctg ctaaccctca
     3241 tatatgcaag tcatcacatc attggtgcaa cagaccaatt tgtcgaatgt tttgtaaaaa
     3301 ttgctttaga atacgcacaa acgcagtcct tgttattaaa tgaattcttg aattattggg
     3361 aggaaaacaa cgaaacactt gcgttacgaa atgacggttt gcgtagcggt gttcgcatca
     3421 tgacggtcca taaatcaaaa ggattagaat ttccggtagt tattattccc attgctgact
     3481 ggtcacttag ttcgaccgga aaaaacaaac tatgggttaa tactgatccc gagcgctttt
     3541 ttggtatgga tacactgctg attccagaag ttaaaaaaat tcaaaactca gatttttatg
     3601 agttttataa aaatgaaatt gaattattgc aactggatgc gataaacctt ttgtacgtcg
     3661 cgatgacacg aagccgtgat catctctata tcatgtgccc tactccaacc gcaaattcag
     3721 acaaagaaaa cgttaggaat gttggtgaaa ttataagcca atttgttatt catgaatcaa
     3781 gtcagttaaa cacatatgaa gcaggtagct ccgcctattc gaatcaaacg cagcacgtaa
     3841 aagaacagga gaagaacctc gtatatacac aaaccgaaac cgttaaagaa aaaccatggc
     3901 gttcttggat ccaattacaa agccgatttg acaatccgag cgatttagat acgttatggt
     3961 atggcgataa aaatacacgc tggggtaacc tcattcattt cgttcttgta caaatgtcca
     4021 gatctgaaga tctgatgaag gcaattgaac acttcaaaaa aataagcttc gtaaatgagg
     4081 aagaagcgca tcatatccac actgttctta caaggttttt tgaaaataaa tctattgtaa
     4141 agctgtttga cgctacggcg gcgattattc gtgaatccga attttttgat actgaaggac
     4201 aattacgccg atttgatcgt atgcaaatcg aaaatgaccg tatcacgatt attgatttta
     4261 aaacagggca aaaaaaagat acgcatgtaa cacaaatgca atcatacatg aaaccgtttg
     4321 ttcaaattca tcaccaaaaa gtacaaggtg cgctgctgta tatagatcca cccgagatcg
     4381 ttgaggtgca tctatgatcg catttcttga tgatatagca acctatttac tcaaagattc
     4441 cccaacgtca ttaaatgatc gttgtgttgt ttttcaaaac caacgcgcaa aattgtattt
     4501 taaaaaatat atcggtcaaa gagctcaacc cggtttgata ttacctgaaa tgctaaccat
     4561 cgaagaatta gccgagcgtt tatcgggtaa acgcattacg gataattttg atcttttgat
     4621 tgagttgtat ttatgccacc aaaaattgaa ccaacccgaa acttttataa aattttatga
     4681 tctgggttta aaaattatag aagattttag cgatatggat atgtatgccg ttgatccggc
     4741 aaagttattt ataaatcttg attatctgaa gtcaactgaa ttttgggata aaaacgatga
     4801 agatttaacc gttttacaac tacgacaaaa aaacttttgg aaatatctgt tcaccctgca
     4861 taaagaattt aaacgagttc ttgacgaaaa agggaaagct tacgccggta tggcttatcg
     4921 cgaagccgtc gatcaaattc gcagtcaaaa ctacacgtta ccttggtcaa aagttttttt
     4981 tgcaggaatt aatgcgctta gtttatgtga ggaagaaata attgatcatt tactaagacg
     5041 aaaacaggct gaaatttttt gggatgctga tgttttttac attaatcaga agcaccatga
     5101 agccggtaac ttcattcgcc gttatatcaa aaaatggcct gaatcagata aacattggat
     5161 cggagatcgt ctatcacgcg ataataaaac aatacggatc acacgcgcat ctcaaaatag
     5221 tgcacaagct cgcgcagcgg ttgttgcact gaaagaaagt gtaaaaaaca aagcgggatc
     5281 cattgccgtc gtattaggtg atgaatctct gttaatacct gttctggaag aattgccgga
     5341 aagcttaact aaatcaacac gcatcaatgt aagtatgggt tatccccttt caatgacgac
     5401 aattcatagc ttcatgttgt gtgtactaca actgcatgcc gatgcattat caaatcaaac
     5461 aaaacgaaaa gagtatacgt ttttacacca atacgtaatt caattttttt cattaccatt
     5521 agttcgcaat ttaattaaag ataaggttta ctctatacac caggccatca taagacaaaa
     5581 ttcaactaaa ttatcacttg aatttattca gtcgcttaca aatctgcgat ctttaaagct
     5641 tgtatttaat gcttgttgcg acattagcga tatgttgaaa aacattgact ctttgattga
     5701 tgaaattaaa aatgaattgt ttataaatcg aatgtatgaa gagcttgagg cgctgtatct
     5761 atggaaggga aagtggacaa taatttgtga cacatatcaa cgccatacga gcttacataa
     5821 tcccgatttg agtgagaatt tagaaacaat catacgccta attcaggacg ctgtgattaa
     5881 tgttcgcatc ccattcgatt cccctgccgt aaattcggat ttgactcaac gtattgaaat
     5941 catgggtatg cttgaaacca gagcattgga ttttgaacat gtcattatct tatccgtcaa
     6001 tgaaggcgta tttcctgcgc cgcgttcgac tcattctttg ataccattta cagctcgtgc
     6061 cgaatcagga atgccaaact atactgaaaa agagtccata tttgcttata acttttacag
     6121 gcttctacaa agagcttcaa ctatagattt gatttatgat gcacaaatca accaaaatac
     6181 aaaaggcgaa cctagtcgat ttatacaaca gcttcgatat gagttagcca aaacatcgcc
     6241 aaacattact attatagaaa atgaattacc ccccctgcca gttataaaat ctagcatcga
     6301 acccttgtta atacaaaaaa caccggccat tatcgatgcc atcacggatg ttttaatcaa
     6361 agggttatct ccaacggcca tcaactctta caatcgatgt tcattgcagt tttatttatc
     6421 gcacattgct aaatttgacg aatccgacga cattaaatct gaacttcaaa gtgatgacct
     6481 tggaaatatc gtccatcacg tatttcagca cttgttcact ccgtatatcg gaaaatctat
     6541 tgccgccaaa gacattagtt c
//
LOCUS       DAOQNQ010000189         6491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_4597_length_6491_cov_6.599441, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000189 DAOQNQ010000000
VERSION     DAOQNQ010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6491
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4597_length_6491_cov_6.599441"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1478
                     /locus_tag="PK754_11125"
     CDS             <1..1478
                     /locus_tag="PK754_11125"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNB10100.1"
                     /translation="DGKRSQLLLLEVATGKISDMIAGDYDVPPIDLGGAHDFGFSPDG
                     KTIIFTMNTDPKIAWSTNNDVWTMPVIGGEPKKISTSPGVDNNGVYSPDGKYIAYCSM
                     ERAGYEADQTDLKLYEVATGKTKNLTEKFDRSVGTLVWSRDGKSIYFDAEDQGFHPVF
                     QMSANGGEPKKITAKTFDLLSDVAQGAIIVRRSSMRMPSEIFAVSEDGKKSMPLSNVN
                     TAKLAALEMAAPEEFWFAGANNTTVHGFLVKPPKFDPAQKYPVVYLVHGGPQNAWNDT
                     WSWRWNPMLFAAPGYVVVMVNPRGSTGYGQQFTDEIRMDWGGKPYEDLMKGIDYICST
                     YSFIDKDRIGAAGGSYGGYMMNWMNGNTDRFKCFVSHASIMNKFNMYGGTEEMWFDEW
                     EMGGPYWEGANRETFEKWSPYNYAQNFKTPTLVVHGELDYRVPVIEGVNLFQVLQRKG
                     IPSKFLYYPDEGHWILKPQNGRLWYQTVHTWFDQWLRQPAP"
     gene            2087..2713
                     /gene="nrfH"
                     /locus_tag="PK754_11130"
     CDS             2087..2713
                     /gene="nrfH"
                     /locus_tag="PK754_11130"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HNB10101.1"
                     /translation="MRKLRELIALVIPPDQWRFPVLILMGILFGLGLVILKISNAVSY
                     LSDDPATCVNCHVMAPQFATWQHGSHARVAVCNDCHVPHDNIIRKYFFKAQDGLRHAT
                     MFTLRMEPQVIQIKEAGAAVVQENCQRCHKNLTERVSAGRTSYAESRHGEGLLCWQCH
                     RETPHGRVNSLASTPYARVPRLAKVMPDWMESFLKNPQKTNLDTTLHP"
     gene            2729..4213
                     /gene="nrfA"
                     /locus_tag="PK754_11135"
     CDS             2729..4213
                     /gene="nrfA"
                     /locus_tag="PK754_11135"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase"
                     /protein_id="HNB10102.1"
                     /translation="MKNIIQTVQEKPWVGWLLFCATVGVVFLVGMFANTVMERRGEEK
                     TLFQMVKPIADWEPRNEVWGENFPREYETYMSTKDTSFRSKHGGSATRDILAEYPNVV
                     ILFAGYAFSKDFKQARGHYYAVEDIRKTLRTGAPQPGTCWTCKSTDVPRVMNERGAPN
                     FYKEKWDNLGHEIVNPIGCQDCHDPKTMNLRITRPALAEAFKRRGMEVEKSNHQDMRS
                     LVCAQCHVEYYFKGKEDKYLTFPWDKGFTAEQMESYYDSVQHVDWVHAVSKAPMLKAQ
                     HPDYEIFKTGIHAERGVSCADCHMPYRSQGGVKFTDHKIQSPLNNIAGSCQVCHRESE
                     ETLRKNVNDRQDKVIEVRSRLEEVLAKAHIEAKTAWEKGATEHEMKPALQLIRQAQWR
                     WDYVAASHGGSFHAPLEAARVLGAGIQKAQEARIILSQVLMAKGVAWPITMPDITTKE
                     KAQAFIGLDMAKLKTAKDEFIKNILPEWDKKAAERQAKMGTKIE"
     gene            4299..5162
                     /locus_tag="PK754_11140"
     CDS             4299..5162
                     /locus_tag="PK754_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNB10103.1"
                     /translation="MRIDKILFPTDFSDCADAALEYAVLTAKKYKASLEFLYVDEATY
                     LLNPMGPYGDNDLHQLSDNVTSYADKKLDDTIQRLHHGVVGHKHVVIGRPYYQIVETA
                     RSIKADMIIMGTHGESGINAIIGSNAERIVRQAPCPVMTIRQKRDANHICRMLIPVDF
                     SDMCRQTMKQIIAYARDFACELTLVYVSVSHKNETEKGIQDDFERFLSRIDITGVIYR
                     TMMVKAHTEGAGIIECAVKNEIDVIAMGTHGRTGLKRVLMGSVTSEVVNNSPVPVVTF
                     RSVKQNNIQHN"
     gene            complement(5367..5870)
                     /locus_tag="PK754_11145"
     CDS             complement(5367..5870)
                     /locus_tag="PK754_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB10104.1"
                     /translation="MKQPTRLHNFIIRKANIEDVPLILSFITELAEYEKLRHEVVATE
                     ALLQKNLFDNNPKAEVIIGELDGNPVCFALFFHNFSTFLGKPGIYLEDLYVRPAVRSQ
                     GIGRIMLAYLAKLAIERNCGRLEWWVLDWNKRAIQFYESIGATPMNEWTVFRVTGKNL
                     EELKDEF"
     gene            complement(5876..6235)
                     /locus_tag="PK754_11150"
     CDS             complement(5876..6235)
                     /locus_tag="PK754_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008685221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB10105.1"
                     /translation="MKKQLPFCPVNATLNVIGGRWKPVILNLLREDTLRFSALKRQIP
                     GITQKMLTQQLRELEQDGIVRREVFPEVPPRVEYSLTEFGQSLKPILKAMCSWGSEHR
                     RYLDRKLKENRSVEEAA"
BASE COUNT         1831 a         1329 c         1549 g         1782 t
ORIGIN      
        1 aagacggtaa acgtagtcaa ctgcttcttt tggaggtggc gacaggcaaa atatccgata
       61 tgatcgcagg agactatgat gtgcctccga ttgatctggg cggtgctcat gattttggtt
      121 tctcgccgga cggtaagacg attattttta cgatgaatac ggatcctaaa atagcctggt
      181 ccaccaataa cgatgtatgg acgatgccgg taatcggcgg tgagcctaaa aaaatttcta
      241 caagtcccgg cgtagataat aatggtgttt attcgccgga cggtaagtat atcgcctact
      301 gttcgatgga acgtgcagga tatgaagcgg atcaaacgga tctcaaactg tatgaagtag
      361 ccaccggaaa aacgaaaaat ctgacggaaa aatttgatcg ttccgtcgga acgctggtgt
      421 ggtcgcgcga tggcaaatct atttattttg atgcggagga tcagggtttc catccggttt
      481 ttcaaatgtc tgctaacggc ggtgaaccta aaaaaatcac cgcaaaaaca tttgaccttt
      541 tgtcggatgt agcgcaagga gcgatcattg ttcgccggag cagtatgcgt atgccgagtg
      601 aaattttcgc cgtaagcgag gacggcaaaa aaagtatgcc gctcagtaat gtgaatacag
      661 cgaagttagc cgcattggaa atggccgctc ctgaagaatt ttggtttgcc ggagcaaata
      721 atacaaccgt acacggtttt ttagtgaaac cgccgaagtt tgacccggct caaaaatatc
      781 cggtagtgta tttggtgcat ggcggaccgc aaaacgcctg gaacgacaca tggagttggc
      841 gatggaatcc gatgcttttt gctgcacccg gttatgtcgt tgtgatggtc aatcctcgcg
      901 gatcaacggg ctacggacaa cagtttaccg atgaaatacg aatggactgg ggcggcaaac
      961 cgtacgaaga tctgatgaaa ggtattgatt atatctgttc gacctatagt ttcattgata
     1021 aagatcgtat cggtgcggcg ggcggaagtt acggcggtta tatgatgaat tggatgaacg
     1081 ggaacacgga tcgtttcaaa tgttttgtaa gccatgcaag tattatgaat aaatttaata
     1141 tgtatggcgg taccgaagaa atgtggtttg acgaatggga aatgggcggc ccttactggg
     1201 aaggtgctaa tcgggagacg tttgaaaaat ggtcaccata taactatgcg caaaatttta
     1261 aaacaccgac gctcgtcgtg catggtgagt tggattatcg tgtgccggtt atcgaaggtg
     1321 taaatctttt tcaagtattg caacgcaaag gcatcccttc caaatttttg tattaccccg
     1381 acgaggggca ttggatactc aaaccgcaga atgggcgatt atggtatcag accgtgcata
     1441 catggttcga ccaatggcta cgccaaccgg cgccgtgaga gaacgcaagc gttacgtgac
     1501 tttctgaagc gacaataccg gtcaacgaat ggatacatcc gagtgagtca aatgggcaac
     1561 gtgcatgagg ggtatgaatt tgacacgacc gcaatgaatt tttgcaaaac aatcgcttca
     1621 cacataggcg ttgtgaataa gatttaagat tgatactttt ttctttttat cctgatggct
     1681 cagcgagctt tctagtatac aagaagaacg ttttgcgtat gccctcccca ctacaacgtt
     1741 ctaatgcgtc attatctggc aagttaacgc aatgcatcgt cgccaacaac gctttcacag
     1801 ttcgaaaaat caaaaaaaac caatattgat tctatttttg tgatgttatt cgttttttaa
     1861 gcatatacaa atcatctgac agtatgggcg tttgtaagtg ataattgtca gctataaaaa
     1921 aaacatcgat tttttttgat atttgtctgt gacttagtca gtctagtaaa tcaatatttt
     1981 tatgactttt gtcatagtgc actctgcgtg atttcagtaa gatgtactgt taaatttgag
     2041 atagtgttat cttttttatg taaaaagttt ttggaatcag agcatcatga gaaaactgcg
     2101 agaacttatt gcgctggtca taccgcctga tcaatggcgg ttccctgttt taattctcat
     2161 gggtattctt tttggcttgg gattggtgat tctcaaaatt tccaatgctg tttcatatct
     2221 atcggatgat ccggctacgt gcgtcaattg ccatgttatg gcaccgcaat ttgcaacctg
     2281 gcaacacgga agccatgcac gcgttgcggt gtgcaacgac tgtcatgttc cgcacgataa
     2341 tataattcga aagtattttt ttaaagcgca agacgggctt cgtcatgcca caatgtttac
     2401 gttacgcatg gaaccgcagg taattcagat caaagaagcc ggtgcggcgg ttgtgcagga
     2461 aaactgccaa cgttgtcaca aaaatctaac tgaacgtgtg tcagcggggc gtacgtcgta
     2521 tgccgaatca cggcacggtg aaggccttct ttgctggcaa tgccaccgcg aaacgccaca
     2581 tggccgcgtc aatagcttgg cctccacgcc ctatgcacgc gttcctcgtc tggctaaggt
     2641 tatgccggat tggatggaat cgtttttaaa aaatccgcaa aaaacaaatt tagatactac
     2701 gcttcaccct taaatcgagg tttactgtat gaaaaacatt atccaaacgg ttcaagaaaa
     2761 accgtgggtc ggctggctgc ttttttgtgc aaccgtaggt gtcgtgtttc tcgtcggtat
     2821 gtttgccaat accgtgatgg agcgtcgagg tgaagaaaaa acattatttc aaatggtcaa
     2881 acctatcgct gactgggaac cgcgcaatga agtgtggggt gaaaattttc cgcgcgaata
     2941 tgaaacatat atgagtacga aagatacatc gtttcgaagt aaacacggtg gaagtgcgac
     3001 ccgcgatata cttgctgaat atccgaatgt agttattctt tttgccggtt atgcgttttc
     3061 taaagatttt aaacaagccc gcggacatta ttatgcggtc gaagatattc ggaaaacctt
     3121 gcgcaccggc gctccgcaac ccggtacgtg ctggacatgc aaaagtacgg atgtaccgcg
     3181 cgtaatgaat gaacgcggcg ctcccaattt ttataaagaa aaatgggata atcttggcca
     3241 tgaaatcgtt aatcccatcg gttgtcagga ttgccatgat ccaaagacga tgaatctccg
     3301 catcacgcgc cctgcattag ccgaagcttt taaacggcgc ggtatggaag tggaaaaatc
     3361 aaatcatcag gatatgcgtt cattggtttg cgcgcagtgc catgtcgaat attattttaa
     3421 aggaaaagag gacaagtatc tgactttccc ttgggacaag ggatttacgg cagaacagat
     3481 ggagtcttat tatgactcgg tgcaacatgt agattgggta catgccgtaa gtaaagcgcc
     3541 gatgctcaaa gcgcagcatc cggattacga aattttcaaa acaggtattc acgcggaacg
     3601 tggtgtatcc tgtgcggatt gccatatgcc gtaccgtagt cagggcggcg tgaagtttac
     3661 cgaccataaa atccaaagcc cgcttaacaa tatcgccggt tcgtgtcagg tgtgccatcg
     3721 cgagagtgaa gagacgcttc gaaaaaatgt gaatgatcgt caagacaaag tgatcgaagt
     3781 gcgttcacgc ttggaagaag ttttagccaa agcacatatc gaagccaaaa ccgcatggga
     3841 aaaaggcgca actgagcatg aaatgaagcc ggcgttacaa ttgatacgcc aggcgcagtg
     3901 gcgctgggac tatgtcgccg cgagtcacgg cgggtcattt catgcaccgc ttgaggcggc
     3961 gcgcgtttta ggcgcaggca tccaaaaggc tcaggaagca cggattatat tatcgcaggt
     4021 tttgatggcc aaaggcgtgg catggccgat cacgatgcct gatatcacaa ccaaagaaaa
     4081 agcacaggcg tttatcggct tggacatggc aaaacttaaa acagcgaaag atgagtttat
     4141 aaagaatatt ttgccggaat gggataaaaa agcagcagaa cgtcaggcca aaatgggcac
     4201 aaaaattgag tgatcattat catcgtttag catgatacgc gtcgttttgt gtttttaaga
     4261 gttgtcctat ttttaagtag gatggcagga gttatgatat gagaatagat aaaatattat
     4321 tcccgaccga tttttcggat tgcgccgatg cggcgttgga atatgccgta ctgaccgcaa
     4381 aaaaatataa agcttcgctg gagtttttgt atgtggatga agcgacgtat ttgctcaatc
     4441 ccatgggacc ttacggggat aatgacctgc accagctatc ggataatgta acaagttatg
     4501 cggataaaaa attagacgat acgattcaac gtctgcatca cggggtcgtg ggacataaac
     4561 acgtggtgat cggcaggccg tattatcaga tcgtcgaaac ggcacgtagt atcaaagcgg
     4621 acatgatcat catgggcacg catggtgaaa gcggtatcaa tgcaatcatc ggctctaatg
     4681 ctgaacgcat agtgcgtcaa gcgccttgtc ccgtgatgac cattcgccag aaacgcgatg
     4741 ctaaccatat ttgcagaatg ctcatccctg ttgatttttc agatatgtgc cgtcaaacaa
     4801 tgaaacagat tattgcctat gcacgggatt ttgcatgcga attgacgctc gtttatgttt
     4861 ctgtttcgca taaaaacgaa accgaaaaag gcattcagga tgattttgag cggtttcttt
     4921 ctcggattga cattacgggt gtgatttacc gtacgatgat ggtcaaagcg cataccgaag
     4981 gcgccggcat catcgaatgt gccgtcaaaa atgaaattga tgttattgcg atgggtacac
     5041 acggacgtac aggattgaag cgcgtattga tgggcagcgt tacctccgaa gtggtcaata
     5101 attcgccggt acctgtggtt acttttcgtt cagtaaaaca aaataacatt cagcacaact
     5161 gagatatttc gttcccatgc gatcgccaag aaataatttg ctcagcaagc acgtcctacc
     5221 aaattgctat atacaatatc gctagattac attcattttt ttgatctatc accatattta
     5281 catttagtgt aaaagatttt tcaaattttt ttataagtcc gtgaaatatt cgctcttatc
     5341 atgtggcaga ttagcttaat cgttgatcag aattcgtctt ttaactcttc caagtttttg
     5401 ccggtaacac gaaacacagt ccactcgttc atcggtgtag cgccgataga ttcatagaat
     5461 tgtatggccc gtttattcca atctaatacc caccactcca gtcgtccgca gtttcgttcg
     5521 atagccagct ttgccaggta agccagcata atacggccaa tcccttggct acgcacggca
     5581 ggtcttacgt atagatcttc caaataaatt ccgggtttac ccaaaaaagt cgaaaaattg
     5641 tgaaaaaaga gagcgaagca tacagggttt ccatccaatt cgccgataat gacttcagct
     5701 tttgggttat tatcgaaaag atttttttgt aaaagagctt ccgttgcgac gacttcatga
     5761 cgaagttttt cgtattctgc cagttcggtg ataaaactta agatcagtgg tacatcctca
     5821 atgttagcct tgcggatgat gaaattatgt agtcgtgtgg gttgtttcat tgtatttatg
     5881 cggcctcttc gacggatcta ttttctttca atttacgatc cagataccga cggtgttcag
     5941 atccccaact gcacatggct ttcaatatag gttttaagct ttgaccaaat tcggtcagcg
     6001 aatattcaac tctcggcggt acttcgggga acacctcgcg acgaacgata ccatcctgtt
     6061 ctaattcgcg caattgctgg gtgagcattt tctgcgttat acccggtatt tggcgtttca
     6121 acgcgctaaa ccgaagtgta tcttcgcgca gaaggttgag tatgaccggc ttccagcgcc
     6181 ctccgatcac attgagtgtt gcattgaccg ggcaaaaggg taattgtttt ttcatgtggt
     6241 tactccaata gtatcctttt agatactata atactttata gtgcttactt gacgaatggt
     6301 tactttagat ttaaattaat actatcatta tagattggca acatataaat ctatttatta
     6361 ggagaaactc atgtcgaatt cgatatttat cgccggagct accggtacaa ctgcctcgca
     6421 attactcaaa cacctcgcca cactcggagt caaagtgaaa gcgggagtcc gtgatataaa
     6481 aaaagctgaa a
//
LOCUS       DAOQNQ010000190         6460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_4624_length_6460_cov_4.908977, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000190 DAOQNQ010000000
VERSION     DAOQNQ010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4624_length_6460_cov_4.908977"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="PK754_11155"
     CDS             complement(<1..892)
                     /locus_tag="PK754_11155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB10106.1"
                     /translation="MIEMILFIVMGVYFASYVFGVMWILMGLFFPHRVFSKKQPKVSV
                     IVAARNEADNIRYCAESLAAQDYPKDQYEIILVNDRSEDQTLAIMQAFVKKFPEQFRI
                     VSVQDKPLDASGKKYALKQGIEAAVGEILLFTDADCTVGPQWITTMASYFSDKTDFVI
                     GFSEVRAVTTFERWQRTDFLAMMIAAAGVSNLGMPWSASGQNLAYRRKAYDAVGGLDP
                     IMHRISGDDILMMNRMRQNGAGIVFAMHPGAYVQTEPVPDWSTFMRQRARWASNGPIM
                     LRYNLWFFCYLTIIYGVCLGL"
     gene            complement(889..2031)
                     /locus_tag="PK754_11160"
     CDS             complement(889..2031)
                     /locus_tag="PK754_11160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB10107.1"
                     /translation="MDFFDLFLWYSAGLYFFGLTVLAIGFCLYKPRRASSYNPLVSVV
                     IAARNEEVNIDRCLAALSEQTYPADRMEIIVVNDRSTDGTENVVQTWSKKIPHLKIVS
                     IREASKSMAPKKFAVDSGIRNSKGEIILCTDADCRPERAWVATMIEHFTPEVGMVLGY
                     SPLWPVHPNSLFENFVACESIGLGVVAASTTALHKPWTVTGRSWGYRRGVYDEVGGFT
                     KIALQVSGDDDLLLGLMKITKWKTAYAIGEQANVRTDPPKSINHFRNQKTRQLSKTRL
                     YSFKMQISVMWYYLFNVALIVSLFFAVLYSEQWRTVVWAWSLKMFSDVIFLSAGAYRF
                     RVLLFLKYYPFLVLIHPVYVLTFGLLGRFGRYTWKDTPVAQGEARS"
     gene            complement(2035..2349)
                     /locus_tag="PK754_11165"
     CDS             complement(2035..2349)
                     /locus_tag="PK754_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNB10108.1"
                     /translation="MKKDSSYPLIWATVMKIPKGKVAAYGQIAYIAGLGRQPRLVGYA
                     LHNIPEGMDIPWHRVINAQGKISFPKGSRHYERQKKMLKTEGIEFNGEKINLDRFGWD
                     PE"
     gene            complement(2346..3650)
                     /locus_tag="PK754_11170"
     CDS             complement(2346..3650)
                     /locus_tag="PK754_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013620573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10109.1"
                     /translation="MKRNLMLGITAALFIASCTKTELPTMNTIAEHYVRLILKIGLHD
                     GDYVDAYYGPDSLKPKSDTALLKTEYALTALLTECDQLLAQLAQIKPDPKQRMDVLRH
                     TYLVKQLTAARTKIEMLNGKKYSFDEESRLLYDAVAPTYDSTHFRAILTELEQKMPGH
                     GNLTDRLEEFRKAFIIPKDKLDAVFQAAIKEGRRRTQQYIPLPEKENFTVEYVNNKPW
                     SGYNWYKGGAYSLIQVNTDLPIFIDRAVDLACHEGYPGHHVYNALLEKTLVRDRGWIE
                     FTVYPLFSPQSLIAEGSANYGIEMALPGAERTKFEKEVLFPLAGIDAKRVDEYYAVQA
                     LVGKISYAGNEAARGFINGIMTREQAIDWMATYGMYARERAEQRLRFIERYRSYVINY
                     NYGLDLVRGYIEKNGGTSDKPETRWKLFEKLLSEPTMPSALQ"
     gene            complement(3663..5051)
                     /locus_tag="PK754_11175"
     CDS             complement(3663..5051)
                     /locus_tag="PK754_11175"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNB10110.1"
                     /translation="MERIASGEPLRAETQADVLTQPATTVAETIKRIRIVRHQWNKAL
                     DYITDLDFVVQNTFGRWSVRDVIAHFIGWERILIERLGHVKNNNAEGVAVFTDEGVDE
                     VNTQWVTRFKESDRSEVMDEWNAAQSDLQHIIQSLTDAMLTSQARGRTVSEIIAGSSF
                     AHEYEHLQKVQSWLQTFQTAPTSAVGVPRVRSDVKPSSFIPNPPFWGDRVVEDISLTD
                     IFPFINEVALFRGQWQFTRGKMSGSEYQSFVDAKVRPVFDAWKKRALEEKLLIPKVVY
                     GYFPCQSDWNDLVIYENDHTTEKLRFTFPRQSDNHHYCISDFFAPKESGKMDVIGCML
                     VTVGQQASDFSAQLFESDNYADYLYFHGLSVETAEALAEYWHRQMRLELGIVSGDHSD
                     TKKLFQQSYQGSRYSFGYPACPHLEDQSKLFTLLDPKRIGITLTDEYHLVPEQSTSAI
                     IVHHPEAKYFNI"
     gene            complement(5040..5750)
                     /locus_tag="PK754_11180"
                     /pseudo
     CDS             complement(5040..5750)
                     /locus_tag="PK754_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630859.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
     gene            complement(5858..>6460)
                     /locus_tag="PK754_11185"
     CDS             complement(5858..>6460)
                     /locus_tag="PK754_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10111.1"
                     /translation="AYAQAVDLLDKELPSHDPATVRDLILERERLHDALLMAEPMRGF
                     EEKKLAVLEKQSTITRSDLAFLAVTLLSLKKDSAGLAFRDVPAQDTMALYYRTAIRCG
                     FLEILPDGKFYPDHIIRRRNLAHYMYRLLSAPDVKLTASPWSDIDPRDPIIAAALWAH
                     QNKVLTPQTGTQFGVDKPVTGLEVKKALERLALLRVEFFK"
BASE COUNT         1768 a         1637 c         1332 g         1723 t
ORIGIN      
        1 taagtccaag gcagaccccg tatatgatcg tgagataaca aaaaaaccaa agattgtatc
       61 gcagcatgat cggcccgttg gatgcccagc gagcgcgctg acgcatgaag gttgaccaat
      121 ccggtaccgg ctctgtctga acataagcgc ccggatgcat tgcaaagaca atacccgcac
      181 cgttttgtcg catacgattc atcatcaaaa tatcatcccc cgaaatgcga tgcatgatcg
      241 gatccaaacc gccgaccgca tcataggcct tgcgacgata agccagattt tgtccgctgg
      301 ctgaccacgg cattcccagg ttggacacgc ccgcagccgc gatcatcatg gctaaaaaat
      361 ctgtgcgttg ccaacgttca aatgtcgtta ctgcacgtac ttcagaaaac cctatgacaa
      421 aatccgtttt atccgaaaaa taagacgcca tcgtcgttat ccactgcggg ccgacggtac
      481 aatcggcatc agtaaataaa agtatctctc cgaccgcggc ttcaatacct tgtttcaaag
      541 catatttctt tccggacgca tccagaggct tatcctgaac cgatacaata cgaaattgct
      601 ctgggaattt tttaacaaac gcttgcatga tcgccaatgt ctgatcttcg gatcggtcgt
      661 taaccaatat aatttcatat tgatctttgg gataatcctg ggcagctaga ctttccgcgc
      721 agtaccgaat attatcggct tcattgcgcg cagctacgat cacggatact ttaggttgct
      781 tttttgaaaa aacacgatgc ggaaaaaata accccataag tatccacatc acgccaaata
      841 catagctcgc gaaataaaca cccattacaa taaataatat catttcgatc atgaccgcgc
      901 ttctccttgt gccacgggcg tatctttcca ggtataccgg ccaaatcgtc ctaatagacc
      961 aaacgtcaat acatataccg gatgaattag aacaagaaac ggataatatt ttaaaaacaa
     1021 taagactcga aaccgatacg caccagccga caaaaatata acgtccgaaa acattttcag
     1081 tgaccaagcc caaacaacgg tacgccattg ctcagaatac aaaaccgcaa aaaaaagtga
     1141 tacgatcagc gccacattaa acaaataata ccacatcaca ctgatttgca ttttaaacga
     1201 atacaggcgg gttttagata actggcgtgt tttctgatta cgaaagtggt ttatgctttt
     1261 cggcgggtca gtccggacat tggcttgttc ccctatcgca taggcggtct tccactttgt
     1321 tattttcatc aaacccagca ataaatcgtc atcgcctgac acctgaagtg caatttttgt
     1381 aaaaccgcca acttcatcat acactccacg ccgataaccc cagctacgac ctgtcacggt
     1441 ccatggttta tgtaatgccg tagtcgaagc ggctacaaca cctaacccta tactttcgca
     1501 agcaacaaaa ttttcgaata acgaattcgg atgaaccggc cacaacggtg aataccccaa
     1561 caccataccc acttccggtg taaaatgttc gatcatggtt gcaacccacg cacgctccgg
     1621 tcggcaatcg gcatctgtac acagaattat ctcaccctta gagttccgta ttccgctatc
     1681 cacagcaaat ttttttggcg ccatggactt ggatgcctca cgaatcgaga cgattttcaa
     1741 atggggaatt tttttagacc atgtttgtac gacattttct gttccgtcgg tcgaacggtc
     1801 attaaccacg atgatttcca tccgatcggc aggatacgtc tgttccgaaa gcgctgctaa
     1861 acagcggtct atattgactt cttcattacg tgccgcaata acgacagaaa cgagaggatt
     1921 ataacttgaa gcacggcgtg gtttatacaa acaaaaacca atggctaaaa ccgtcaatcc
     1981 aaaaaaatat aaccctgcgg aataccaaag aaacaaatca aaaaaatcca ttccctactc
     2041 cggatcccaa ccaaaacgat cgaggtttat tttttcccca ttaaactcga taccttcggt
     2101 tttaagcatt tttttttgcc gctcgtaatg ccggctacct ttaggaaaag aaattttacc
     2161 ttgtgcattg atcaccctat gccacggtat atccatgcct tcgggaatat tatgcagcgc
     2221 atatcccacc aatcttggct gtcggcccag tcccgcgata taagcgatct gaccgtaagc
     2281 ggcgacttta cctttaggaa ttttcataac cgttgcccaa ataagcgggt atgaactgtc
     2341 ttttttcatt ggagcgccga cggcatggtg ggttctgaca gtagcttctc aaacaatttc
     2401 caccgcgttt ccggtttgtc cgaggtgccg ccattctttt caatatagcc gcgaaccaaa
     2461 tcaaggccgt aattataatt aatcacataa ctgcggtagc gttcgataaa acgcaaacgt
     2521 tgctctgcgc gctcccgtgc gtacataccg tacgtggcca tccagtcaat ggcttgctcg
     2581 cgtgtcatta taccgttgat aaaaccgcgt gcggcttcat tgcccgcata acttattttt
     2641 ccaacaagcg cctgcacggc atagtattca tcaacgcgct tggcatctat cccggcgagc
     2701 ggaaataata cttctttttc aaatttcgta cgttccgcac cgggcaaggc catctcaata
     2761 ccatagttgg cgctaccttc ggcgatcagc gactgcggac tgaacaaagg atagacagta
     2821 aattcgatcc aaccccgatc ccgtacgagt gtcttttcaa gtaaggcatt gtaaacatga
     2881 tgaccaggat acccttcgtg acaggccaga tctacggcac gatcaataaa aatcggcaag
     2941 tcggtattca cctgaatcaa actgtaagcc ccgcctttat accaattgta tccgctccag
     3001 ggtttattgt ttacatactc gaccgtaaaa ttttcttttt ccggcagcgg gatatattgc
     3061 tgagtgcggc ggcgaccttc tttgatagcc gcctggaaaa ccgcatccag tttatctttg
     3121 ggaataataa aagccttacg aaattcttcg agacgatccg tgagattgcc gtggcccggc
     3181 attttttgct ccagctccgt gaggatcgct cggaaatggg tcgaatcgta cgtcggtgcg
     3241 acggcatcat aaagcaaacg cgattcttca tcaaacgaat atttctttcc attgagcatt
     3301 tcgattttcg tacgcgccgc cgtcagttgt ttaaccaaat acgtatgacg taatacgtcc
     3361 atgcgttgct tcggatcggg ttttatttga gccagttgcg caagaagttg atcacactcc
     3421 gtgagcagag cggttaacgc atattcggtt ttaagtaaag ccgtatccga tttcggtttg
     3481 aggctgtccg gcccgtaata cgcatccaca taatcgccat cgtgcagtcc tattttaagg
     3541 atcagacgca cataatgttc tgctatcgta ttcatagttg gtaactctgt tttagtacag
     3601 ctggcaataa acaaagccgc ggtgatgcca agcattaaat tgcgtttcaa atgtattcct
     3661 cgttatatat taaaatattt ggcttccgga tgatgaacga tgatcgcact ggtagattgt
     3721 tcgggtacaa gatgatactc atcggtcagc gtgatgccta tgcgtttagg gtccagcagt
     3781 gtaaacaatt tactttgatc ttccagatga gggcatgccg gatacccaaa actataacgt
     3841 gaaccctgat acgattgctg aaagagtttt ttggtgtcgc tatgatcccc gctcacaatg
     3901 cctaactcca gtcgcatttg gcgatgccaa tattcagcca aggcttcggc cgtttccacg
     3961 ctgaggccgt gaaaataaag ataatcggcg taattatccg attcgaaaag ttgcgctgaa
     4021 aaatctgacg cttgttgtcc gaccgtgacc agcatacatc cgatcacgtc cattttgccg
     4081 gattctttag gcgcaaaaaa atcagaaata cagtagtgat gattatccga ctgacgcgga
     4141 aatgtaaaac gcaatttttc ggtagtatga tcgttttcat aaataaccag atcattccaa
     4201 tccgattggc aaggaaaata tccgtaaacg actttgggaa taagaagttt ttcttccaaa
     4261 gcgcgttttt tccacgcatc aaatacgggg cgtactttcg catctacgaa actctgatat
     4321 tcactgccgc tcatttttcc tctggtaaac tgccactgac cgcgaaataa agcgacttcg
     4381 ttgataaacg gaaaaatgtc cgttaacgaa atatcttcga ctacgcgatc cccccaaaaa
     4441 ggcggattcg gaataaacga tgaaggcttc acatcggagc gaacacgtgg tacaccgacg
     4501 gcgctcgtcg gcgcagtttg gaaagtttga agccaggatt gcactttctg caaatgctcg
     4561 tattcatgtg caaatgaaga gcccgcgatg atttcagata cggttcgccc ccgtgcttgt
     4621 gaggtcagca tcgcatccgt cagtgattgg atgatgtgtt gtaaatcgga ttgtgccgca
     4681 ttccattcgt ccatcacttc ggatcggtcg ctttctttga aacgggtcac ccactgcgta
     4741 ttgacttcgt ccacgccttc atccgtaaat acagccacgc cctccgcatt gttgtttttt
     4801 acgtgaccca atcgttcgat cagtattctt tcccacccga taaagtgagc gatcacatca
     4861 cgcacggacc aacgaccaaa tgtgttttgt acgacgaaat ccaaatcggt gatatagtct
     4921 aaggccttat tccactgatg acgtacgata cgaatacgtt tgatcgtttc ggccactgta
     4981 gttgcgggtt gcgtgagcac gtccgcctgc gtttccgccc gcagcggttc accactggca
     5041 atgcgctcca taacgcgtaa tcctgaaaat gcatcttcgc aataaaacaa cggcgcctca
     5101 tacacggaac gtaaatcttc ctctacgtaa cgacgagtca acgctgcacc gcccagcaaa
     5161 acgggaacct gtatgccgcg ttcggccatc acttcaagat tttctttcat gatcagagtt
     5221 gactttacca aaagcccact catcccgatc gcgtcggctt tgtgctcatg cagcgattgc
     5281 agcatagttt ctaccggtac tttaataccg agattgatga ctttataacc gttattggtc
     5341 agaatgatat ccacgagatt ttttccgata tcgtgcacat cgcctttgac cgtagcaagc
     5401 accatcgtac ctttttgaga accggatgat ttttccataa atggttccag gtaagccacg
     5461 gcggttttca tcacttccgc cgattgcaaa acaaaaggca gctgcatctc gcctttgccg
     5521 aacaaatcgc ctacgacctt cattccttcg aggagaatgg tattgataat ttcaagcggt
     5581 ttataattct gcatggccaa tttgagatca tcatccaaac cttttttttc accttcgata
     5641 atccggtttt tcaaccgttc ttcgaccgtg gcggcggttg ttgatttaga cacgctgact
     5701 tcattttttt tagaagcaaa gtgattgatg atttcaatca acggatcata cacgactttt
     5761 tgtgacgaat cagacacagg aaccctcggt tattttaaat gcaccaaaca atatataagc
     5821 acaaactacg cggaacaata agtttctgtt atcaattcta cttaaaaaat tcgacccgca
     5881 aaagcgccag acgctctaac gcttttttaa cttccaatcc ggttacgggc ttatccactc
     5941 caaattgggt tccagtttgt ggtgtaagca ctttattttg atgagcccac agagctgctg
     6001 caataatcgg atcacgcgga tcaatatcgg accatgggga cgcggtaagc ttgacgtccg
     6061 gcgcacttag taaacgatac atataatgcg caaggttacg gcgtcgtatg atgtggtccg
     6121 gataaaattt tccgtccggt aatatttcta aaaagccgca ccgtatcgcg gttcggtaat
     6181 acaacgccat cgtgtcctga gccggtacgt cacgaaatgc caaaccggcg ctatcttttt
     6241 tcaagctcaa taacgttacc gctagaaacg ccaaatcact tcgtgtaatc gtcgattgtt
     6301 tttccagaac ggctaatttt ttttcctcaa acccccgcat cggttcggcc atcaaaagcg
     6361 catcatgcaa acgttcccgt tccaagatca aatcgcgaac ggtcgccgga tcatgcgaag
     6421 gcaattcttt gtcgagtaaa tcaacagcct gcgcataagc
//
LOCUS       DAOQNQ010000191         6336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_4765_length_6336_cov_5.838402, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000191 DAOQNQ010000000
VERSION     DAOQNQ010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6336
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4765_length_6336_cov_5.838402"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PK754_11190"
     CDS             complement(<1..488)
                     /locus_tag="PK754_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017743410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNB10112.1"
                     /translation="MTKRCVSHFLLAIFLVATGSSHVWTQKFECFVLQGPQKNLTGVK
                     KIALMDFIGKNGSGVKLADYLTSEMLVENRGIRDIAGFFSTKEGKTFIKGVKTNIFTV
                     VERSKIQQLLKEQNFSMTDAVDANQAAQVGKLLGVDAIITGSVDYSFTDTPEEFKSKK
                     KNG"
     gene            complement(567..917)
                     /locus_tag="PK754_11195"
     CDS             complement(567..917)
                     /locus_tag="PK754_11195"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNB10113.1"
                     /translation="MIHRRLNEQPGCISELAKQIGISRSTLYRLKNGDPKIEARDFTI
                     DRIIRALNISYEIKNGKVIEVENRRLIAVSHDPDEMRLFKEWVMKIDHIVANNLNSND
                     YELIRLLVCRCLKP"
     gene            1210..1752
                     /locus_tag="PK754_11200"
     CDS             1210..1752
                     /locus_tag="PK754_11200"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNB10114.1"
                     /translation="MEVITTITDCTMTEQNVQELASKLYDAFKEQIRRKVKYRIKRQE
                     EIDDVVAEVYLAIVDSLHKGNFDTSRNAGSYIHRIVENKINTYFRQRKNRLDKFVDID
                     VNELQDNNSNYLEIKSNIEAFKNELSKLKNNHREVLKLRYFEGLSYGEIAKAIGSTEK
                     TVGNLIRYATDKLKKNLKGK"
     gene            1786..2502
                     /locus_tag="PK754_11205"
     CDS             1786..2502
                     /locus_tag="PK754_11205"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNB10115.1"
                     /translation="MLIDRYLHNELSEKDRLSFEEHYFICSECFEELLETEKLKTTIK
                     LGGKELFAVASGLNKRDRIFKYILVAASFALIFFTLYQFFPEKKSGNFSNDSRIALID
                     TVKKVKEDDSGDKEIHEKTLNPKKNIGFLVENYTPNADFDNIIRQNFRDKNSLQIISP
                     LVDAIISDSVIFDWKPKETKNLELVILNNREEQMQRISINAPFIWNTTTVMPGLYYWK
                     LETSTDLLYIGRFIIKKPLM"
     gene            complement(2515..5835)
                     /locus_tag="PK754_11210"
     CDS             complement(2515..5835)
                     /locus_tag="PK754_11210"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNB10116.1"
                     /translation="MSAFSKIRYTAYNEIDFYKLRENMNSYTAFLLILLFPAKINSQN
                     LREHDSLRIVRLFQSAENSNKSGNIDSALFYNRYAASLALKYKLWNQYSTAVAQTTKN
                     LIQKNKAKDAISECDSAIQHIIRSDNKSFGNISRLLNNKSIALRRLSDYNQALESVIK
                     AVSLLQENNVEPNKEILADCFGSMTNIYYDMGKYRDAMIANENQRRILSSIFGRNNTR
                     IAGSYSNQGLNFHAIALYDSAITYFDSSISIHEACGNSESLDYASALSNKGISFFEKG
                     DFKKALLLYNQALELRKRLVGDKFLYIAGNYANIGVTYHELGDYDASIIYMEKALSIL
                     NQLNITKHPFIGGIFANIGISYRSKKDYETAVKYVFNALRMRQSLFEMAHPHIGDSYN
                     DLGNIYFDLGDYKSALIYYDSASYIKSQTTSASSPQMAKLYSSKAALYSITGILDSAK
                     HYYKLAAESWKLIYGEKHYMLSLSIMHLADLEELNNLDSTIHMYQTAICTNTLDFDDT
                     NVFNNPNVESQILSYDVMVQLLSNKARYLLKIYSEKKDTMYVVGAYNALEIAHLLINQ
                     KQRTIITDNAKFSNVSIGQDVIDDLLETLYMLDEIRGTKNAAEMYKYVVKAKSSIFIE
                     SLKDLEITKGVGDGKLLMRENDLREQVSQREIELAKKLESNKFEMNDHVLGIKNDLFD
                     LKEEHASVISELKKTFPSYYNLKYPSSEIDISEIQRGLNDNTALIEYFMGKKKWYVFV
                     ITNDDFEVFRFSKDTVFDPTIDQYLKSVRTINKPEFVSSSYSLYKKLIAPLDKLIDSK
                     KHWIIIPDGKLYSVPFESLISAVQNGEKDFTKLKYLIHRHDIRYHYSAALFLRASENK
                     IVSQSPTFLGFAPIFSDSLKNGHILTTNTTVLDTLSGIRYRFVTDDSKELMPLPHSET
                     EVANIVSLFEKKAWPARAYFHRQATESNFKNFSEAGKFLHIATHGLINLKKPKLSALL
                     FSQPQNTNQGENSINRNGEPVDDGVLYSGEIYNLNLKADLVTLSSCESGIGEYIRGEG
                     MMTLTRGFLYAGSNNILFSLWKVPDESTSRLMTEFYRQCLTNKNYSESLREAKLLMIK
                     KQATSFPLNWSGFVLIGR"
BASE COUNT         1994 a         1204 c         1045 g         2093 t
ORIGIN      
        1 ccgttcttct tttttgattt aaattcttcc ggggtatcgg taaaagaata atccacgctc
       61 ccggttataa ttgcgtcaac tccaagaagt ttaccgactt gagccgcttg atttgcatct
      121 acggcatcgg tcatgctaaa attttgttct ttcaataatt gttgaatctt tgatcgctct
      181 acaactgtaa aaatatttgt ttttactcct ttgatgaagg ttttaccctc ttttgttgaa
      241 aagaatccgg cgatatctct gattcctcta ttttcgacaa gcatctccga ggtcaaataa
      301 tcagctaatt ttactccgga tccgtttttc ccgataaaat ccataagcgc tatctttttc
      361 accccggtta agtttttttg cgggccctgc aatacaaaac attcaaattt ttgagtccaa
      421 acatgggaag aacctgttgc aactaaaaaa attgcaagca ggaaatgaga cacacatctt
      481 tttgtcataa aaataccttt ctttataaag ttgaagaaat ttaaaagtgc tttattaaaa
      541 gtgctttttt atcggtgtgt ttgcattcat ggctttagac aacggcacac caacaatcga
      601 atcaattcgt aatcattact attcaaatta tttgccacaa tgtgatctat tttcattacc
      661 cattccttaa ataatctcat ttcatccggg tcgtggctca ctgcaattaa tcttctattt
      721 tccacttcga tcaccttccc gttctttatt tcatagctga tatttagcgc acggatgatc
      781 ctgtcaatag taaagtccct tgcttcaatt ttgggatcac cgttttttaa acgatacaag
      841 gtgctccggc tgataccgat ttgtttggct aattcactga tacaaccagg ctgctcgttg
      901 agccgtctgt gaatcaattt cagtaattct tctttgattt tagtgccccc tgaaatgact
      961 atctcctcat tctctaaaat ttacgggttg atactaaagt atatcaagtc tcatattgtt
     1021 cttttacgta tcattttgtg tcatatacag ggaacacatc atttatagat gtggacattg
     1081 atataacaca gtcgtccata agaagtattc gctacaaacg cagaaatgct gaattttatt
     1141 attttttttt atttttagat gagttagaat gtattgtaaa atcgcacggt tatatttttc
     1201 aggcatccta tggaagtaat cactacaatc actgactgca caatgacgga gcaaaatgtt
     1261 caggaactcg cttcaaagct atatgatgct ttcaaggagc agatacgccg taaagtgaag
     1321 tatcgaatta aaagacagga ggaaattgat gatgttgttg cagaagtata tcttgctata
     1381 gtcgatagcc ttcacaaagg caactttgac acttcgcgaa atgctggctc ctatattcat
     1441 cggatagtag aaaataaaat caacacttac tttcgacaac gtaaaaaccg cctagataaa
     1501 ttcgttgaca tcgatgtaaa cgaattacag gataataaca gcaattactt agaaataaag
     1561 agcaatattg aagcatttaa gaacgaattg tctaagctaa agaataacca ccgcgaagtt
     1621 cttaagcttc gttattttga gggtttgtcg tacggtgaaa tagccaaagc cattggttca
     1681 actgaaaaaa ctgtaggtaa tttaatccgt tatgcaacgg ataagttaaa aaagaattta
     1741 aaaggtaaat aaatggaatc atgctcatat aagaaccaac gaagcttgtt gattgatcgt
     1801 tatttacata acgaattgag cgagaaagat cgcctgtctt ttgaagaaca ttattttatt
     1861 tgtagtgaat gttttgaaga actccttgaa acggagaaat taaaaacaac gataaagttg
     1921 ggtgggaaag agttatttgc cgtggcgtca ggactcaata aacgagacag aattttcaaa
     1981 tatatactag tagcggcttc atttgccttg atctttttta ctttatatca attttttcca
     2041 gaaaaaaaat ctggcaattt ttccaatgat tcaagaattg ccttgatcga taccgtaaag
     2101 aaagtgaagg aagacgatag cggcgacaaa gaaatacacg aaaaaacgct taacccaaaa
     2161 aagaacattg gtttcttagt tgaaaattac accccaaatg cagatttcga caatatcata
     2221 cgtcaaaatt tcagagacaa aaactcatta caaataattt ctccactagt ggacgcaatt
     2281 atcagcgatt ctgtcatttt tgattggaaa ccaaaagaaa ctaaaaattt agaattggta
     2341 atactcaata accgagaaga acaaatgcaa cggatttcaa taaatgcccc ttttatatgg
     2401 aacaccacga ccgtaatgcc aggtctttac tactggaaac tggaaacctc aactgacctt
     2461 ttatatatag ggcgatttat cataaaaaaa ccgctaatgt gacttaagaa acagctatct
     2521 tcctatcaaa acgaaaccgc tccaatttag tggaaaagag gtagcttgtt tctttatcat
     2581 cagcagtttt gcttcacgta aagattcgct atagtttttg ttcgtcaaac attgacgata
     2641 aaactccgtc attaacctac tcgtactttc atcaggtact ttccagagcg aaaataaaat
     2701 attattcgaa ccagcgtaaa ggaaaccgcg tgttagtgtc atcatcccct cgcctcttat
     2761 atattctccg atacccgatt cgcaactact taaagtaact aaatctgctt ttaagtttag
     2821 attatatatt tcgccggaat acaatactcc atcatcaaca ggctcgccat ttcgattgat
     2881 cgaattttct ccttgattcg tattctgagg ttgcgaaaat aaaagtgcag agagtttcgg
     2941 ttttttcaga ttaataagac cgtgcgtagc aatatgcagg aactttccgg cttcggagaa
     3001 atttttgaag ttagattcgg tggcttgccg atgaaaatat gcccgtgctg gccaagcttt
     3061 tttttcaaac aaagaaacaa tatttgcaac ttccgtttca gaatgcggca aaggcatcaa
     3121 ttctttacta tcgtctgtaa caaatctgta cctgatgcca ctcaaggtat caaggacggt
     3181 cgtatttgtg gttaaaatat gaccgttttt taacgaatcg ctgaatatag gcgcaaaacc
     3241 aaggaatgtt ggactctgtg atacaatttt attttcagaa gcccttaaaa ataacgccgc
     3301 cgaataatga taacgtatat cgtgacgatg gataagatac ttcaatttcg tgaaatcttt
     3361 ctcaccgttt tgaacagctg agatcagcga ttcgaaaggc acggaataaa gcttcccatc
     3421 aggaatgatt atccaatgct tttttgaatc tatcaatttg tcgagcggag caattaattt
     3481 cttatataga ctgtaactgg acgaaacgaa ttccggctta tttattgtac gaacagattt
     3541 caaatattga tcgatggtcg gatcgaacac agtatctttt gagaatcgaa aaacttcaaa
     3601 atcgtcatta gttataacaa acacatacca ctttttttta cccatgaaat attcaattaa
     3661 ggccgtgtta tcattcagtc cgcgttggat ttcagaaata tcaatttcag aagatgggta
     3721 ttttaaattg tagtaacttg ggaacgtttt ttttagctcg ctaatcacgc tagcatgttc
     3781 ttcttttaaa tcaaataaat cattcttgat acccaaaaca tgatcattca tttcaaactt
     3841 gtttgattct aattttttgg ctagttcaat ctctctttga gaaacctgct ctctgagatc
     3901 attttccctc attaaaagct tcccatcgcc gacacctttt gtaatttcta aatctttaag
     3961 actttcgata aaaatactag acttggcttt tacaacgtat ttgtacattt ctgcagcatt
     4021 ttttgttcct cgaatttcat ccagcatgta aagtgtttct aataaatcat cgatcacgtc
     4081 ttgtccaata ctcacatttg aaaatttagc attatccgta ataatcgttc tttgtttctg
     4141 atttattaat agatgtgcaa tttccaatgc attataagct ccgacaacat acatcgtatc
     4201 ctttttttcg ctgtaaattt taagcaaata tcgggctttg ttactaagca attgaaccat
     4261 aacatcatat gataatattt gtgattctac attaggatta ttaaaaacgt ttgtatcgtc
     4321 aaaatctaga gtattagtac atatcgctgt ttgatacatg tgaatcgtcg aatctaagtt
     4381 atttaattct tctaagtcag ctaaatgcat tattgaaaga gaaagcatgt aatgcttttc
     4441 accataaatc agtttccaag attccgctgc taatttataa taatgcttgg cgctgtctaa
     4501 aatcccggta atggaataaa gcgctgcttt gctgctataa agttttgcca tctgaggtga
     4561 actagccgag gttgtttgtg acttgatata agatgcagaa tcataataga tcaaagccga
     4621 tttatagtcg cccaagtcaa aataaatatt gccaagatcg ttatagctat ctccaatgtg
     4681 agggtgtgcc atttcgaaaa gcgattgccg catcctcagt gcattaaata catacttgac
     4741 agcggtttca taatcttttt tgctacggta gctaattccg atatttgcaa aaatacctcc
     4801 gataaaagga tgttttgtta tgttcagctg atttaatatg gacaacgcct tttccatata
     4861 aataatagat gcatcgtaat ctcctaactc atgataggtc acacctatat tcgcataatt
     4921 acctgcaata tataaaaact tatctccaac aagccgtttt cgtaattcca atgcttgatt
     4981 gtataataat aatgcttttt taaaatcccc tttttcaaaa aaagaaatcc ccttattgct
     5041 caaagcactt gcatagtcca aactttctga attaccacat gcttcatgaa tcgatatact
     5101 ggaatcaaaa taagttatcg ctgaatcgta taaggctatt gcatgaaaat tgagtccttg
     5161 attggaataa gaaccggcaa tacgagtatt atttctacca aaaatacttg aaagaatacg
     5221 acgttgattc tcatttgcaa tcatggcatc tctgtattta cccatatcat aataaatatt
     5281 tgtcattgag ccaaaacagt ctgctaatat ttctttgttt ggctctacat tattttcttg
     5341 gagcaaagaa acagctttta ttacactttc aagcgcttga ttgtaatcag acaatcgcct
     5401 caaagcgatt gatttgttgt ttagcaatcg tgaaatatta ccaaatgatt tattgtcact
     5461 tctaataata tgctgaattg cgctatcaca ttctgatata gcatctttag ctttgttttt
     5521 ttggattaag ttctttgtcg tctgggcaac agcagtagag tactgattcc aaagtttata
     5581 ttttaaggcc aaactcgcag cgtatctatt ataaaatagg gcgctgtcaa tattccctga
     5641 cttatttgaa ttttcagcag attggaataa acgtacaata cgtagagaat catgttctct
     5701 tagattttga ctattaattt tagccggaaa aagtaaaatt aataagaaag ccgtgtaact
     5761 attcatgttt tccctcaatt tgtaaaaatc aatctcatta tatgcagtat atctaatctt
     5821 tgaaaaagct gacattgatc cctctcaata aaaagacgaa aaagaaataa aaaactattg
     5881 ttagagtgta acttagtata tccggtctta gtcggtttgc tggaaaccta ctgatattgt
     5941 tctctctttt tcgcctcagt accgaattta atttccctaa tttagaaaaa cataccattt
     6001 ccccatctca cttcttttcc caacttctga aaatttgcac gcttcagaaa attggcgcat
     6061 ggattcgatg accatcttat tcgctatcaa cgcacctgag ccgtttcata gttccttcaa
     6121 atataacacg cgacccccgc cttaatcgtg ttgagcatat cggccttgtc caacgcaata
     6181 gtcgctttat ccgccggagt tctttttgta gtgccatatg ccctgttaat ccggcttttg
     6241 aaatttatta cgatcaggta ttagccgaag gacatgcatt gattccgtag ctaaatgcaa
     6301 ctcattcttt agatcgaata tgctctacga tcgttt
//
LOCUS       DAOQNQ010000192         6315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_4794_length_6315_cov_5.737540, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000192 DAOQNQ010000000
VERSION     DAOQNQ010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4794_length_6315_cov_5.737540"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..1096)
                     /gene="fdhD"
                     /locus_tag="PK754_11215"
     CDS             complement(257..1096)
                     /gene="fdhD"
                     /locus_tag="PK754_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017608668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNB10117.1"
                     /translation="MESATRTYLIKKFSDGLDRDHADTLVTEEPLSIAVAYMERTVEI
                     AVTMRTPGHDADLALGFLAAEGLLHARDGSPDYTIVQKITSPEENIITLHVNESAFAR
                     MAEADQLRRYSFVQSSCGICGSRSISDLRAKGFRRIEDAAVFMAEPILALPDRLRREQ
                     KIFEQTGGLHGAGLFDNEGNLLCAREDIGRHNAVDKIIGWTFMQRRLPLHGHLLLVSG
                     RVSYEIVQKALSVGIPCIAAVSAPSSLAVKSAHEFGMTLIGFLRSGHYNIYCGAQRIV
                     QHA"
     gene            1350..2300
                     /locus_tag="PK754_11220"
     CDS             1350..2300
                     /locus_tag="PK754_11220"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNB10118.1"
                     /translation="MEEKKKSPHKYVTLLVMFDAVSDPRSFRLKVSTLRVMLGTLAVV
                     GLVLAGSLIYYFSFAYKVFYYDELERKYEKLADDNTRIKQIEREYRKVKQENEKIRIV
                     FGLLKNMPRDSTYEGRSTYENFTAPSEMGYYPGKDLNDPMHEKMAEDISSDVGRIGID
                     YLSYARVVPTLMPVNSRFIARGYQDNRYSDPSATRIHRGVDIVAEEGAPVKAASEGRV
                     IVSEWFTDYGNTIVLYHGFGFFSVYKHLQFRLKETDDAVKTGEVIGTVGKTGRLATGI
                     HLHFEIWRDGVPRDPATFIPQIRDALVITVKADSLAKKSS"
     gene            2320..2760
                     /locus_tag="PK754_11225"
     CDS             2320..2760
                     /locus_tag="PK754_11225"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNB10119.1"
                     /translation="MAFGEKNTNNTAGAGLNLISRGTVINGNISASTSIRIEGEIKGK
                     IVCHDAVTIGTTGMVEGDIDAKSVIVGGKVIGNVTSQDRLMMESRSGIQGNIKARRLV
                     VEEGAVFEGRCSMKDTKDKPLVINENANRDMFEEPEAARLAKAQ"
     gene            2766..3071
                     /locus_tag="PK754_11230"
     CDS             2766..3071
                     /locus_tag="PK754_11230"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNB10120.1"
                     /translation="MPTGKHTTQSLSETMHALGPWIGWGWQFALTLGVLTWGGHWLDG
                     YFETKVLFTLAGIFLGLAGGFIQLIRMVRNLPKPERHHHGEERHSDRVTKPTNEEKE"
     gene            3068..3457
                     /locus_tag="PK754_11235"
     CDS             3068..3457
                     /locus_tag="PK754_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10121.1"
                     /translation="MSQKLFLMVVSLITVIVLLILNAFIETAQEFRVVLCASVVALAH
                     VLCGFYMTRWAFTKSNKLFMSVVMGGMGIRMMLVGIILVIVYKLVAIDIKLFIVTFGV
                     YYLLFQIVEIYFVNRGLQMKKLNNNTI"
     gene            3477..4382
                     /gene="atpB"
                     /locus_tag="PK754_11240"
     CDS             3477..4382
                     /gene="atpB"
                     /locus_tag="PK754_11240"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNB10122.1"
                     /translation="MPMLTFWQAASQEAPKAADKAEKVDIGGTIIHHITNSNEIELPI
                     LGYVKLPHFDPIHIGTMTIDLSPTKHVVMIWLVALLLLVIFSILARPKLIPRGLYNAM
                     EALISFVREEIVRPNFGAKTDRYIHYFLTLFFFILFMNLLGLIPYGSTATGNIAVTAT
                     LAVVTFFVTQYTAIKSQGIGGYLKHLTAGVHWLLWPIMIPVEVVGLFTKPFALSVRLF
                     ANMTAGHVVILSLLGLIFVLKTYFVAPISILFALFIYVLELFVAFLQAYIFTLLSSLF
                     ISMGMHHEHHEEHHDAEHPVHAGHH"
     gene            4455..4682
                     /gene="atpE"
                     /locus_tag="PK754_11245"
     CDS             4455..4682
                     /gene="atpE"
                     /locus_tag="PK754_11245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNB10123.1"
                     /translation="MGDLGFGFLAAGIGAGLATIGAGLGIGKLAASALEGSARQPESA
                     GDIRTTMIIAAALIEGVALFAEVICILLALK"
     gene            4788..5336
                     /gene="atpF"
                     /locus_tag="PK754_11250"
     CDS             4788..5336
                     /gene="atpF"
                     /locus_tag="PK754_11250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNB10124.1"
                     /translation="MKTLLLLHMISGGGEHKGGLLDIDPGLIFWTIVTFVILLVLLRL
                     TAWGPIIKMLEEREEKIKNSLEEAEKARREAAALTEKNQEIMAKAEREAQEVARRAKE
                     NAEKLKTEIMDQAKAEAARLVAEARTQIENEMNSAISALRKEVGDMAVTAAGKILATN
                     LDADKHKKLIDDFIKDMPVGRN"
     gene            5376..5924
                     /gene="atpH"
                     /locus_tag="PK754_11255"
     CDS             5376..5924
                     /gene="atpH"
                     /locus_tag="PK754_11255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNB10125.1"
                     /translation="MKNSATARRYAKALFDISVEQNALDAVYADMQSIATMIEQSPEF
                     RAFLHNPVIPNAQKKDAMKALFDKKIHTEVYRFMHLICDHDRENILGDMIEAFETLRN
                     EKLGFIHAEVISVVDMTPDQVKGVTEKIKALTGKTPQLVFKKDASLIGGFMVRIGDTM
                     IDGTVKHQLERLKSAFTAVYHH"
     gene            5988..>6315
                     /locus_tag="PK754_11260"
     CDS             5988..>6315
                     /locus_tag="PK754_11260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012467220.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNB10126.1"
                     /translation="MAVSVRPDEVSGILKKQLASFEREMDTYETGTVLQVGDGIARIY
                     GLTKAMASELVEFPNGVVGMLLNLEEDNVGAVLFGDSSGIKEGDTVKRTGRLAEVAVG
                     PEMLGRV"
BASE COUNT         1620 a         1442 c         1652 g         1601 t
ORIGIN      
        1 aacggtcgcg ggtgatcgcc gtgacttgcg attggacaag cccttgttcg acattgacgg
       61 agcggtggat tttgatttga ccgctgacgt cggatgacgg catcagcagt atacaaaaaa
      121 tcagagttag tttttttagc gggtgcatcg gggttgatgt taccatatta aactgcgcca
      181 tcagacgtga tttatgaaaa taagaaccga ttattttcga gatgcgcgga tacggatacg
      241 tgacctgacg aaacaactat gcgtgctgta cgatccgctg cgcaccgcag tagatattgt
      301 agtggccgga gcgaagaaat ccgatcaatg tcataccgaa ctcgtgtgcg gatttgacgg
      361 ccagactgga cggggcggaa accgccgcaa tacacggtat gccgacgctc aacgctttct
      421 gcacgatttc gtaactgacg cgtccgctga ccaacagcag atgaccatgg agcggcaaac
      481 gtcgctgcat gaaagtccag ccgatgattt tgtccacagc gttatgccgt ccgatatcct
      541 cgcgcgcgca gagtaagttt ccttcattat caaataatcc ggcgccgtgc aggccgcctg
      601 tttgctcgaa tattttttgt tcccggcgca ggcgatcggg taaagcgaga attggctcgg
      661 ccataaaaac cgccgcgtcc tcgatgcggc gaaatccctt agcgcgcaaa tcgctgattg
      721 agcggctccc gcagataccg cagctggatt ggacaaacga atagcgacgc aattggtcgg
      781 cctcggccat gcgggcgaag gcggattcat tcacatgcag tgtgataata ttttcttccg
      841 gtgatgtgat tttttgtacg atcgtatagt ctggggaacc gtcgcgggcg tgaagtaaac
      901 cttcggcggc caaaaatccc agcgccaagt cggcgtcgtg accaggcgtg cgcatcgtaa
      961 cggcgatttc gacggtgcgt tccatgtacg caaccgcgat ggacagtggt tcttcagtga
     1021 cgagcgtgtc ggcatgatcg cggtccaaac catcgctgaa tttttttatg agataggtgc
     1081 gtgttgccga ttccacatga acctcgaagt ttcgcacaaa ctacccaaaa agggcttcaa
     1141 tttcactaat tttatggtat ttttatgcta taaagatgtt aatttccgga tcggcttata
     1201 attaagaaaa aaattgacaa atcatgttct tttagatata ttgcgttgct ttttggaaaa
     1261 aaaggcgtat gttctgtaaa aatttagatt tagcatcgtt ttaaagcgat ataaacgcga
     1321 tccgatcggt catcaaaaac gggttgaacg tggaagaaaa aaagaaatcg ccgcacaaat
     1381 atgtcacact tctggtgatg tttgatgccg tttccgatcc ccggagtttt cgccttaaag
     1441 tttcaacgct acgcgtgatg ctcggtacat tggctgtagt aggtcttgta ctggccggtt
     1501 ctttgatata ctatttttcg tttgcctata aggtatttta ttacgacgag ctggagcgta
     1561 aatacgaaaa gctcgccgac gataataccc gcatcaagca gatcgaacgc gagtaccgta
     1621 aggtcaagca ggagaatgaa aaaatccgca tcgtgtttgg tttgctgaaa aacatgccgc
     1681 gggactcgac gtacgaaggc cgctcgacgt atgaaaattt tacggcgccg agtgaaatgg
     1741 gatattatcc cggcaaagac ctcaacgatc cgatgcatga aaaaatggcg gaggatatca
     1801 gcagcgatgt cggccgtatc ggtatagatt atttatccta cgcacgtgtc gtgcctacgc
     1861 tgatgccggt taacagccgc tttattgcgc gcggttatca ggacaaccgt tacagcgatc
     1921 cgtcggctac acgcatacat cgcggggtag acatcgtggc ggaggaaggc gcgccggtca
     1981 aggccgcttc cgaaggtcgg gtgatcgttt cggaatggtt taccgactac ggcaacacga
     2041 tcgtcctgta tcacggattc ggtttttttt cagtgtacaa acatttgcaa tttcgcctga
     2101 aagaaacgga tgatgcggtc aagacgggtg aggtgatcgg cacggtcggt aagaccgggc
     2161 gtttggcaac aggtattcat ttacattttg aaatatggcg cgacggcgtg ccccgggatc
     2221 cggcgacttt tataccgcag atccgcgatg cgctggtgat tacggtgaaa gccgactcac
     2281 tcgccaaaaa aagcagttaa actaaacgaa agaacgccca tggcgtttgg tgaaaaaaat
     2341 acgaacaata ccgccggtgc aggtctcaac ctgatcagcc gcggtaccgt gatcaacggc
     2401 aatatcagcg cctcaacgag cattcgtatc gaaggcgaaa tcaaaggcaa aatcgtttgc
     2461 catgatgccg tcacgatcgg tacgacgggt atggtcgaag gcgatattga tgccaaaagt
     2521 gtgatcgtcg gcggcaaagt aatcggcaat gtgacttcgc aggatcgcct gatgatggag
     2581 tcgcgttccg gtatccaagg taatatcaaa gcccgccggt tggtagtcga agaaggcgcg
     2641 gtgttcgaag gtcgttgttc gatgaaagat acgaaagata agccgctcgt gatcaacgag
     2701 aatgcgaatc gcgatatgtt tgaggaacct gaagctgcgc gtttagctaa agcgcagtag
     2761 gttttatgcc cacgggtaaa catacgaccc aaagcttatc ggaaacgatg catgcgcttg
     2821 ggccgtggat cggatggggt tggcaatttg ccctgacgct cggcgttttg acttggggcg
     2881 ggcactggtt agacggatat tttgaaacga aagttctttt tacgctggcc ggtatttttt
     2941 tgggattggc cggcggtttt atacagttga tccggatggt tcggaatctt ccgaagccgg
     3001 aacgacatca ccacggagag gagcgtcatt cggaccgcgt aaccaagccg acgaatgaag
     3061 agaaggaatg agtcaaaaat tgtttctgat ggtcgtatcg ctgatcaccg tcattgttct
     3121 tttgatattg aatgccttca ttgaaacggc acaggaattt cgagtcgtgc tttgtgcgag
     3181 cgttgtagca ttggcgcacg tgctttgcgg cttttatatg acgcgttggg cttttactaa
     3241 gtcaaacaaa ctttttatga gcgtcgtgat gggcggcatg ggcattcgta tgatgttggt
     3301 cggcattatc ctcgtgatcg tgtataaatt ggtggcgatc gatatcaagt tgttcatcgt
     3361 tacatttggt gtgtattacc tgctgtttca gattgtcgag atttattttg taaaccgcgg
     3421 gctgcagatg aaaaaattaa ataataatac tatataggat attcgtacgc atgctcatgc
     3481 ctatgttaac cttctggcaa gccgcttcgc aggaagcgcc caaagccgcc gacaaagcag
     3541 aaaaagtgga tatcggcggg accatcatcc accacattac caattccaat gaaatcgaac
     3601 tccccatatt gggttatgtg aaactgccgc attttgatcc cattcatatc ggcactatga
     3661 cgatagacct cagtccgacc aaacatgtcg tgatgatatg gttggtagcg cttttgctat
     3721 tggtgatttt ttccatcttg gctcgtccca aactgattcc tcgcggcctc tacaatgcca
     3781 tggaagcgtt gatcagcttc gtgcgcgaag agattgtgcg ccctaatttc ggtgctaaaa
     3841 ccgaccgcta tatccattat ttcctcacgt tgtttttctt tattctgttt atgaatcttc
     3901 tggggcttat cccgtatggt tcgacagcaa ccggtaatat cgccgtcaca gcgaccttgg
     3961 ccgtcgtgac gttttttgtc acgcaatata ccgctatcaa gtcgcaaggt atcggcgggt
     4021 atctgaaaca tcttacggcc ggcgtgcatt ggcttttgtg gccgatcatg attcccgtcg
     4081 aagtagtggg gctttttacc aaacctttcg cgctcagcgt tcgtcttttt gccaatatga
     4141 cggcaggcca cgtcgtgatt ctctccctgc ttggtttgat cttcgtatta aaaacatatt
     4201 ttgtcgcgcc gatttctatc ctgttcgcgt tgtttattta cgtattggaa cttttcgtcg
     4261 cctttctgca ggcgtacatt tttacgctgt tatcgtcgct ttttatcagc atgggtatgc
     4321 accacgaaca ccatgaagag catcatgacg cggagcatcc ggtgcatgcg gggcatcact
     4381 gaatagataa cgaatgcaat taacaaatac ataaacgaac tcatttacaa catcatttaa
     4441 cggaggaaac tacaatgggc gatttaggat ttggcttctt ggccgccggt atcggcgcag
     4501 gtctggctac gattggtgcg ggtttgggta tcggtaaact ggccgcttcc gccctcgaag
     4561 gcagtgcgcg tcaaccggaa tccgccggtg atatccgtac gaccatgatc attgcagccg
     4621 cccttatcga aggtgtggcg ctttttgcag aagttatctg catcctgttg gcattgaagt
     4681 aatcgtattg attgtacgtt ggaatacggc gggagttaaa tccctcccgc cgtttgttta
     4741 atgatgttgt tgtaaactga gttgtaactg aaaaggtttt gtacgctatg aagacattac
     4801 tgcttctgca catgatctcc ggcggcggcg aacataaagg cggattgctg gacatcgatc
     4861 cggggttgat cttttggacc atagtcacct tcgtcatttt gctcgtgctt ttacgcctga
     4921 ccgcatgggg cccgatcatc aagatgctcg aagaacgcga agaaaaaata aaaaattctc
     4981 ttgaagaagc ggaaaaagcc cgccgtgaag cggccgctct gaccgaaaaa aatcaggaaa
     5041 tcatggcgaa ggccgaacgt gaggctcagg aagtcgcgcg ccgcgccaaa gaaaatgccg
     5101 aaaaactgaa aaccgaaatc atggatcagg ccaaagccga agcggcccgt ttggtagcgg
     5161 aagcccgtac gcagatcgaa aacgaaatga attctgcgat cagtgcgttg cgcaaagaag
     5221 tcggtgacat ggcggtcacg gccgccggca aaatcttggc gaccaacctg gatgccgata
     5281 aacataaaaa actgatcgat gattttatca aagacatgcc ggtggggcgt aattaattaa
     5341 ccggtacaac gttaaagtat taataccaaa gtaatatgaa aaattccgca accgcacgac
     5401 gctacgccaa agccctgttt gacatctcgg tcgagcagaa cgcgctggat gccgtctatg
     5461 ccgatatgca gagcatcgcc acgatgatag aacagtcgcc ggaattccgt gcttttttgc
     5521 acaatccggt gatccccaat gcgcagaaaa aagacgcgat gaaagcgctg tttgataaaa
     5581 aaattcacac cgaagtgtat cgctttatgc atttgatctg cgatcacgac cgtgaaaaca
     5641 ttttaggcga catgatcgaa gcgtttgaaa cgctgcgtaa tgaaaaactc ggtttcatcc
     5701 atgcggaggt tatctccgtc gtggatatga cccccgatca ggtcaaaggc gtcaccgaaa
     5761 aaatcaaggc cctgaccggc aaaacgccgc aactggtttt caaaaaagat gcgtcattga
     5821 tcggcggatt tatggttcgc atcggagata cgatgattga cggtacggtc aagcatcagc
     5881 tggaacgcct caaatcggcg tttacggctg tgtatcacca ttaatgatgt aaaacattcg
     5941 aacgaataga gacaaataaa tataatataa cacaaggaac ctaaatcatg gcagtttctg
     6001 taagaccaga tgaagtatcc ggcattctca agaagcaact tgcttctttt gaacgcgaga
     6061 tggataccta tgagacgggt accgtgctgc aagtcggtga cggtattgcc cgtatttacg
     6121 ggttgaccaa ggccatggcg agcgagctgg tggaatttcc caacggcgtc gtcggcatgc
     6181 ttctcaatct cgaagaggat aatgtgggtg ccgtactttt cggtgactct tccggcatca
     6241 aagaaggcga tacggtcaaa cgtacgggcc gccttgccga agtggccgtc ggaccggaaa
     6301 tgctcggacg tgtcg
//
LOCUS       DAOQNQ010000193         6268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_4840_length_6268_cov_6.445357, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000193 DAOQNQ010000000
VERSION     DAOQNQ010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4840_length_6268_cov_6.445357"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PK754_11265"
     CDS             complement(<1..613)
                     /locus_tag="PK754_11265"
                     /inference="COORDINATES: protein motif:HMM:NF013663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNB10127.1"
                     /translation="MRLGIIINTEKESVIAMLPDFLEWLTARHYKFMVNTDAEEFIRA
                     RTSDKTVFADARTLVEASDVLISFGGDGTLLSTAKMVGTAQKPILGVNVGKLGFLTEI
                     ETKELHHAIERLHDGKYTREKRMVLEITLGTRTFYALNDAVIMRGNDGRVIQIGVDVN
                     NAFLNNYIADGLIIATPTGSTAYSLSANGPIMSPTIEAIIINPI"
     gene            complement(615..1574)
                     /locus_tag="PK754_11270"
     CDS             complement(615..1574)
                     /locus_tag="PK754_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10128.1"
                     /translation="MKNLFNTKIYALLIIAATTLQAQSDVSVTARTNRDTVAIGENFV
                     YELNIRVPDTSVPIGEPAADFSDAEVVQIRRLEPLTKDGTREEKIQYILSGFKLEPIA
                     IPGPSLRYLIGKDTLFLRANDKTIFIRSELDSAMTDIQPEKPIFYGEVRWWLIALYVL
                     ITIGVLAGAAWLIYRWYKKRQELKNRPKPEPVVIPKKPHEAALEALDALKISGYPERG
                     DMKLWHSEASTIIRIYIEKQFGVPALETTTSELIPSLKRSKIIADADVTILRRMLEVC
                     DLAKFAKYMPSVDDCYELLELAYSFVRHTMPAGEAPPEVKTGV"
     gene            complement(2072..3310)
                     /locus_tag="PK754_11275"
     CDS             complement(2072..3310)
                     /locus_tag="PK754_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10129.1"
                     /translation="MKKYYIFVCFFFFLSMAIQAQMFNNKPYFKLYVELNDSSNQYVY
                     KYLRLSSSNYKKRLLKEIWEQIGARLPKKFVTVDYRAYKTENKYNENITICNDSSLIE
                     EAIWLGFIYNIRLEKDTSESYNRKINLTFDYSLHRTLTEDKDYSGYVSANLDSMSEFD
                     FLQNLSEKITEPVLTNLPQALAIDEAKYGYAILLRINDSYSGGPAVRNKHYSVIRLYS
                     SPSITSWKSKDYKIGDKDLNKNNKSISFGIQYINSIGLSVGVESSVDQILYSKPAGLI
                     KTKSSIHTPDESVASYSIMGSIGYHYLLFSGRLQLSGDLFFGQSNYKTLTQYPYLHIR
                     GYYTSSESFAQGVKLTYIPFSSVRTAIFGSIKWSEEKLELKKIKQKGYSTTDDQIVIE
                     RNPVFNKTQISAGLAFDIKL"
     gene            complement(3329..4351)
                     /locus_tag="PK754_11280"
     CDS             complement(3329..4351)
                     /locus_tag="PK754_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10130.1"
                     /translation="MEERELLVFAYEYVYQDMPGEAFVAIPFSDSTIAVAGYTDSAGS
                     SIAFFARFSIDGEMKWRVDPLNGKKGSILGGIKSSDGTVILTGEIEGSTGDKDIVVLK
                     TTYEGEVLWLKTWDSTLADKGLAVKEAEDGKIHVLGLAQNFKLVILILDGQGNEIHVN
                     WTELVGHNNCRSIIPRNGGAGYVVAESGEWMSTFSYNGTGQINLLRQSRRGAKASSAA
                     DIGGGLFAYVGNSSNGSFLSYVNPIAFSEATVIVDDIHSVLNSVYSNKDGSIDVVGNN
                     GDELFVKSYDKNRMLLKSFFYGKSEIVFSARVMTSITGNEKIFVGSATTTNTLNKYVL
                     ILKSLE"
     gene            complement(4801..5571)
                     /locus_tag="PK754_11285"
     CDS             complement(4801..5571)
                     /locus_tag="PK754_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HNB10131.1"
                     /translation="MCLVVWALAAHPQYRLIVAANRDEFYARPTEVSKWWADHNNLLA
                     GRDLQGGGTWMGVTRGGRFAVITNFRDPSSEKKRATTRGRLVEQFLRSEEAPDIYVQA
                     VVSQGESYNGFNLIVADPSRMAYVTNTQPGWSWVEPGIYGLSNHRLDTPWPKVARTKK
                     RFSEILRSDEPDTNAMMEMMSDRETAPDKDLPVTGVGIEWERKLSPVFIQMPHYGTRT
                     TTIFMEDHTGRCTWIERTWDPIRAQFKLKKDTWTKMVV"
     gene            complement(5574..6107)
                     /locus_tag="PK754_11290"
     CDS             complement(5574..6107)
                     /locus_tag="PK754_11290"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB10132.1"
                     /translation="MIVSLSSKWSRIEASKTYFVTCVQAMNEHQSHWRPKLDAWHALD
                     VLQHLVLVEEGMAKQLHHREAILHKKTVLPKIIGLPAYWLTFRMGMRVKAPVKSVLPQ
                     EVMTLAMCVQRWDAVRDVMKNFLETITSETEGAIYFYHPITGQRTCEQMLDFLYDHLR
                     HHQAQMRRIMHHPKYPA"
BASE COUNT         1768 a         1446 c         1235 g         1819 t
ORIGIN      
        1 agatcggatt gatgatgatc gcttcgatcg tcgggctcat gatgggcccg ttggctgaaa
       61 gcgaatacgc ggtcgaaccc gtcggcgtcg cgatgatgag tccatcggca atataattat
      121 tgagaaacgc attattgaca tccacaccga tttggatcac gcggccgtca ttgccacgca
      181 tgatcacggc atcattgagc gcataaaatg tgcgtgtacc gagcgtgatc tccagtacca
      241 tacgtttttc acgcgtatat ttgccgtcgt gcaggcgctc aatcgcatga tgcaactctt
      301 tcgtttcgat ttccgtcaaa aaacccaatt tgccgacatt gacgccgagg atcggttttt
      361 gtgcagtacc gaccattttg gccgtggaga gcaatgtgcc gtcgccccca aaggatatca
      421 ggacatccga cgcttcgacg agcgtgcgcg catcggcaaa caccgttttg tcggaggtac
      481 gggcacggat aaattcttcc gcatcggtat tgaccatgaa cttgtaatgc cgcgcggtga
      541 gccactccag aaaatccgga agcatggcga tcacactttc tttttccgta ttgatgataa
      601 ttcccagacg catgtcaaac tcccgtttta acttcaggcg gcgcttcgcc ggccggcatc
      661 gtgtggcgca caaacgaata agcgagctcc aacaattcat aacaatcatc taccgacggc
      721 atgtacttgg caaacttggc cagatcgcac acttccagca tgcgacgcaa tattgtcaca
      781 tcagcatctg cgataatttt ggatcgtttt aaagacggta tgagttcgga tgtcgtcgtc
      841 tccagcgcgg gtacgccaaa ttgtttttcg atatagatac gaatgatggt agatgcttcg
      901 ctatgccaga gtttcatatc cccgcgttcc ggataaccgc tgattttcaa agcatccagc
      961 gcctcgaggg ccgcttcgtg cggttttttg ggaatcacaa ccggttcggg tttcggtctg
     1021 ttttttaatt cctgcctctt tttgtaccaa cggtaaatca accacgccgc gcccgccaat
     1081 acaccgatgg ttatcaggac atacagcgcg atcaaccacc aacgcacttc gccgtaaaaa
     1141 atcggcttct ccggttggat atcggtcatc gcactgtcta attccgaacg aataaaaatc
     1201 gtcttatcgt tcgcccgcag aaaaagcgta tctttaccga tcagataacg cagcgacggc
     1261 ccgggaatcg caatcggctc gagtttgaaa ccggataaga tgtactggat tttttcttcc
     1321 cgtgtaccgt ctttggttag gggttccaaa cgccgaatct gtaccacttc cgcatcggaa
     1381 aaatcagccg ccggttcgcc aatgggtacg gatgtatccg gcacgcggat attgagctca
     1441 tacacaaaat tttcaccgat cgcgacagta tcccgatttg tacgcgccgt caccgacacg
     1501 tccgactggg cttgcaacgt cgtagccgcg atgatcaaaa gcgcgtatat tttggtatta
     1561 aataaatttt tcataaacgc ggctaatgta taaaaattga taaccaatgg caatgcaagg
     1621 ctttaagttt ggatatattc ggatcggcta caaaaaatgc agttcgttta taaatccgtt
     1681 cgatgccatg cgtgtcggtg caacgtaaat cgttctaaaa cgggatccca cgtgcgctct
     1741 atccatgtac atcgcccggt atgatcttcc ataaaaatcg tcgtggtgcg cgtaccgtag
     1801 tgaggcaata tatatcggag aggttgtata ttatgattta gaaaaaacag ccgaaactaa
     1861 ttgcgtcatc tatcgaaata cataatcttc aaaccaaact aagtcatcag aatacaggcc
     1921 ggtacagatc atcttaggta atcaggctta aatctgtctt actgacggca atatctttat
     1981 aatgatgaca cacaaccttc ccatatttct ttcagaaaaa tctaactttg attccaaaat
     2041 ctaacgcaac actttttgtg tgaatttatg tctacaactt gatgtcgaat gccaatcccg
     2101 cactgatttg cgtcttattg aaaacaggat tacgttctat tacgatttga tcgtccgtcg
     2161 tactatatcc tttttgcttt atttttttca actctagttt ttcttcgctc cattttatac
     2221 tcccaaaaat tgccgtacgc accgacgaaa acgggatata tgtcagtttc acgccttgtg
     2281 cgaaactttc tgaggaagta taatatcctc ggatatgtaa gtaaggatac tgcgttaagg
     2341 ttttataatt cgattgacca aaaaacaaat ctcccgataa ttgcaaccgc cctgaaaaca
     2401 acaaataatg atacccaata cttcccatga tcgaataact tgcaacggat tcatctggtg
     2461 tatggatact gcttttcgtc ttaatcaaac ccgccggttt tgagtataaa atctgatcaa
     2521 ctgaagattc aacgcctaca gacaatccaa tcgagtttat gtactggata ccaaaagata
     2581 tagatttgtt gtttttattc aaatctttat ctccgatttt gtaatccttc gatttccaac
     2641 ttgttatgga cgggctagag tataatcgaa tcacagagta gtgcttgttt cttacagcgg
     2701 gtccaccgga ataactatca ttaatccgta gtaaaatggc gtatccatac tttgcttcgt
     2761 caattgctaa tgcctgagga aggttagtta aaacaggctc ggtgattttt tccgataaat
     2821 tttggagaaa atcaaattca gacatagaat ccaaattagc tgaaacgtac cctgagtagt
     2881 ctttatcttc tgtaagcgtc ctgtgcaatg aataatcaaa cgtcaaattg atttttctgt
     2941 tgtaactttc agatgtgtcc ttttctaatc ggatgttata tataaagccc aaccatatgg
     3001 cttcttcaat caaagatgaa tcattacaaa ttgttatatt ttcattatat ttattttcgg
     3061 ttttgtaagc tctgtaatca acagtcacga atttttttgg caatcgagcg ccaatttgtt
     3121 cccagatttc ctttagcaac ctttttttat agtttgagga agataacctt aaatacttat
     3181 aaacgtattg atttgacgaa tcatttaact caacatacaa tttaaaataa ggcttattgt
     3241 taaacatttg cgcttggatt gccatcgaaa gaaagaaaaa aaagcaaacg aaaatataat
     3301 attttttcat aaaactctaa ttccttaatt actcaagtga ttttaaaatg agtacgtatt
     3361 tgttaagtgt atttgtcgtc gtagcgctac cgacaaatat tttttcattc ccggtgatgg
     3421 atgtcatgac cctggccgaa aagacaatct cactttttcc ataaaaaaac gatttcagca
     3481 gcattctatt tttatcatag cttttcacaa atagttcgtc accattattt ccaacaacat
     3541 ctatggagcc atctttatta ctataaactg aattcaatac agaatggata tcatctacta
     3601 taacggttgc ttcactgaaa gcaatcgggt ttacgtatga tagaaaacta ccgtttgagc
     3661 tattcccaac ataggcaaac aatcctcctc ctatatccgc ggcagatgaa gctttagcgc
     3721 ctcgccttga ttggcgtaat aaatttattt ggcctgtacc gttatatgaa aaagttgaca
     3781 tccattcgcc ggactcagcc acgacatatc cggcgccacc gttacgggga attatagaac
     3841 gacaattatt atgccccacc aactcagtcc agttgacatg tatttcattg ccctgaccat
     3901 caaggattag gatcaccagt ttgaagtttt gggctaaacc taaaacatgg attttcccgt
     3961 cttcggcctc ttttacagcc aagcctttat ccgctagagt tgaatcccat gtttttagcc
     4021 aaagaacttc cccctcatag gttgttttta atacaacgat atctttgtct ccagttgaac
     4081 cctcgatttc accggtaaga atgaccgttc catccgaaga ttttatcccc ccaagaatag
     4141 agcctttttt tccgttaagt ggatccactc tccatttcat ttcgccgtca atcgaaaaac
     4201 gcgcaaaaaa cgctattgac gaaccggcgc tatccgtgta gcctgcaacg gcaatagtac
     4261 tgtctgagaa tggaatcgca acaaaagcct ctcccggcat atcttgataa acgtattcgt
     4321 aagcaaacac taataattcc ctttcttcca aagaacaacc aatgaacgct agtaacatga
     4381 aaaaaaaatg gagaggattg aagagttttt ttcttattgg gttcatacat tacactatta
     4441 atttgttatt ttcttattcg tgaattaaaa aacattattc tgaattgaga aaggaaagca
     4501 tgtagtgatt gcccccgacg tacaaataca aattttacag atgctgacaa attcagccaa
     4561 gaacacatag tttagtaccg gtaaattttt taaactctag atggattttt tggattaaat
     4621 ttaaacagac tctactaaaa gatagaaaac tttacgagcc cactaataac aacaatatat
     4681 ttattacaaa aacaagccgt atatgctaat aaaaattctc tgaaagtcaa tttcaaaaaa
     4741 atcatttttt gacgagtcat cacaaaaaac tgtcctcgtc tatagtacct ttttactcga
     4801 tcaaaccacc attttagtcc acgtatcctt tttcaattta aattgagcgc gtatgggatc
     4861 ccacgtgcgc tctatccatg tacatcgccc ggtatgatct tccataaaaa tcgtcgtggt
     4921 gcgcgtaccg tagtgaggca tctgaataaa taccggtgac agtttgcgct cccactcaat
     4981 acctaccccg gtcaccggca gatctttgtc cggcgcagtt tcacgatcgg acatcatctc
     5041 catcatcgca ttcgtatcgg gttcatcgct acgcagaatt tcagaaaacc gttttttggt
     5101 gcgggcgact ttgggccacg gtgtatccag acgatgatta ctgagaccgt agatacccgg
     5161 ttcaacccat gaccaaccgg gttgcgtatt ggtgacatac gccatacggg acggatcggc
     5221 gacgatgaga ttgaagccgt tgtaggactc gccttgggaa actacggcct gcacatatat
     5281 atccggcgct tcctcggagc gaagaaattg ttcgactaat cgcccgcgtg tcgtagcgcg
     5341 ttttttttca gaactcggat cccgaaaatt ggtaatcacg gcaaatcgtc cgccgcgcgt
     5401 cacacccatc cacgtaccgc cgccttgcaa atcacgtccc gcgagtaggt tattatgatc
     5461 cgcccaccac ttcgaaactt ccgtcgggcg cgcgtaaaat tcgtccctgt tggcggcgac
     5521 gatcaggcgg tattggggat gggctgctaa agcccagaca acaaggcaca tggtcaggcc
     5581 ggatatttgg ggtggtgcat aatacgacgc atctgcgcct gatgatgccg gagatgatcg
     5641 tataagaaat ccagcatttg ttcacaagta cgttgcccgg tgatcgggtg ataaaaataa
     5701 atcgctcctt cggtttctga cgtgatggtt tcaagaaaat ttttcatcac atcacgcacc
     5761 gcatcccaac gttgcacaca catcgctaac gtcataactt cttgtggcag tacagatttg
     5821 accggcgctt tgacccgcat ccccatacga aacgtcaacc aataagccgg cagaccgatg
     5881 attttaggta aaacggtttt tttatgaaga atggcttccc gatgatgcaa ttgtttggcc
     5941 atgccttcct cgactaaaac cagatgttgc aatacatcca gtgcatgcca cgcatcgagt
     6001 ttaggtcgcc aatgcgattg atgttcattc atagcctgca cacaggtcac aaaataagtt
     6061 ttgctcgcct caatgcggga ccatttggaa gataaactta caatcatagg tttcgtgcgt
     6121 tttgccgttc aaaaatcata cgattatatc gcgatctgac gtagattaaa agagtttttt
     6181 tattctacaa tatacccggt gtttttttag aaaatttttt gtaataaata tagctctata
     6241 tgtactactc ttatcaatat gggtaatc
//
LOCUS       DAOQNQ010000194         6110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_5032_length_6110_cov_5.672007, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000194 DAOQNQ010000000
VERSION     DAOQNQ010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5032_length_6110_cov_5.672007"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..815)
                     /locus_tag="PK754_11295"
     CDS             complement(51..815)
                     /locus_tag="PK754_11295"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014244354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNB10133.1"
                     /translation="MKNDWKKILEQLERGEIRAATYDNGFWRANVAVKEAILSAFREG
                     KLIAMNRPTYGRYGGFVDKDSMIPRSFQPKQKIRLVPGGSSVRRGAYVAPGVVIMPPS
                     YINVGAYIDEQTMVDSHVLVGSCAQVGKRVHLSAGVQIGGVLEPVGSAPVVIEDDAFV
                     GADAVIVEGICVGTGAVIAPGVVLSRGVTVYDAVHEKELGRGARIPDLAVVVPGTRPL
                     SSSWAKERGLATACAIIVKYRDAKSNAALQLEEALR"
     gene            complement(812..1702)
                     /gene="dapA"
                     /locus_tag="PK754_11300"
     CDS             complement(812..1702)
                     /gene="dapA"
                     /locus_tag="PK754_11300"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNB10134.1"
                     /translation="MKNLQSVTLWTAIVTPFLDNGKIDFLSFERLLRRQEAAGNGVVI
                     LGSTGEGLALNDDEKREVVRFTGTCKLHVPVMVGVGGFNLDATQRWIEENNQHAFIHA
                     YLLVTPLYAKPGARGQCSWFAALLEASQKPCMLYNVPSRTGVKLHTSVLRELKDHSRF
                     WAVKEASGSVTEFQDYREAAPGVAFFSGDDGLTPFFAAAGAVGLVSVMSNVWPEASAE
                     FVYRCVNRDTGVLLPTWRTASDAMFLASNPVPAKALLHELGLITSAYVRPPLSMEDLP
                     DRNKLIMADSLVQRWMEQYG"
     gene            complement(1706..2401)
                     /locus_tag="PK754_11305"
     CDS             complement(1706..2401)
                     /locus_tag="PK754_11305"
                     /inference="COORDINATES: protein motif:HMM:NF017024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNB10135.1"
                     /translation="MPKISLIGNGKTGGAVERLLPPDEIAAVFNSKNPPTVETLKNSD
                     VAIVFVPGHVLNDLVPVLIQSAVPVVCGSTGFEWTMDVRRAVENSGATWITASNFSMG
                     MQWMFRMAREFRRLESQWAGSEAEIFEVHHIHKKDKPSGSALSIRQHYGKQIPIHSDR
                     TGDVVGFHSLVLATASEKLMLSHHVEDRSVFARGAIWTAQKLIEKRSRPQDYPSGIFP
                     LEDVFETWLNEGQ"
     gene            complement(2383..3765)
                     /gene="lysC"
                     /locus_tag="PK754_11310"
     CDS             complement(2383..3765)
                     /gene="lysC"
                     /locus_tag="PK754_11310"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011864585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HNB10136.1"
                     /translation="MNDLIIAKFGGTSMGTAEAMRQSARIVIANNRIRVVVVSATSGT
                     TNALLKVYHDLCACDTVSADQALKKIEDRHRTMCHALELDSSAVDELIVAAQNHMSLF
                     SASENREVWLDAWLSFGERLASRIFGKMLFHLKNNVTEADARSFIITDEVFGKAEPQP
                     DRIKTACLKKLLPLCGEQVIVTQGFIGATTDGRTTTLGRGGSDYSAALLGEALSAKEV
                     LIWTDVPGIFTMDPNLVPAAQTIPKISFSEAAELANFGAKVLHPATLWPAIRKGIHVF
                     VGSTFEPEAGGTWIEPDVNDKPLVRAIAMRKHQTLITVSSLRMLNTHGFLAKMFGVLA
                     DHKLSVDLVTTSEVSVALTIDGTSLGSSGEKISEKQELLQDLRRFADVAIEENLTLIA
                     LVGNNLQQTPGIAARTFDAIAGLNVRLICQGASSYNMCFLMHQSDAPQAVSALHRAFI
                     ESEVVCLKSA"
     regulatory      complement(3882..4050)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(4233..4682)
                     /locus_tag="PK754_11315"
     CDS             complement(4233..4682)
                     /locus_tag="PK754_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNB10137.1"
                     /translation="MSKGTLNKVIIIGRLGGDPELRYTPSGAPVATFNVATNESYKDK
                     EGKLIESTDWHRIVAWNKLAEICGQYLKKGSLVYVEGKIKTRSYDDKDGQKKYVTEIR
                     ADNMQMLGAKMDGGRGEASLPHPADMGGGMDHAADAGGSSGSDDLPF"
     gene            complement(4709..5782)
                     /gene="recA"
                     /locus_tag="PK754_11320"
     CDS             complement(4709..5782)
                     /gene="recA"
                     /locus_tag="PK754_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNB10138.1"
                     /translation="MADEMVLSDKDKKAALDRTLTDIVKEYGKGSIMKLGERTAMQVE
                     VFSTGSISIDAALGVGGIPRGRITEIYGPESSGKTTLSLHVIAEAQKVGGVAAFIDAE
                     HALDAIYAKRLGVDIDNLLVSQPDNGEQALEITERLVRSNAVDVIVIDSVAALVPRSE
                     IDGEMGDATMGVQARLMSQAMRKLTGAVSKSKTCVIFINQVRDKIGVMFGNPETTTGG
                     RALKFYTSIRVDIRRIGQIKAGENLVGNRTKVKVVKNKLAPPFKEVEVDLMYGTGISR
                     EGDLVDLASTMNIVQKSGTWFSFEDEKIGQGRENAKQYLTDNPVVLKKIETRVRQELG
                     IAPAATEKESPKPAPTASEKKSK"
BASE COUNT         1444 a         1685 c         1421 g         1560 t
ORIGIN      
        1 ccggtcggcg gcctcgtgta acagtgtaag gtgatgcgcc gtcaatgctt tcatcgcaac
       61 gcctcttcca gttgtaacgc ggcgttgctt ttcgcatcgc ggtatttgac gatgatcgca
      121 caagccgttg cgagaccgcg ttctttggcc caggacgatg atagcgggcg cgtaccgggt
      181 acgacgacgg ccaaatcggg aattctggcg ccacgtccca attctttttc atgtacggcg
      241 tcgtacaccg tgacaccgcg cgaaaggacc acaccgggtg cgatcacggc tccggtgccg
      301 acgcaaatac cctctacgat caccgcgtcc gcacccacaa aggcatcgtc ttcgatcacc
      361 acgggagcgc tgccgaccgg ctccaatacg ccgccgattt gtacaccggc gctcagatgt
      421 acacgttttc cgacctgcgc acacgaaccg accaacacat gactgtccac catcgtttgt
      481 tcgtcaatgt atgcgccgac attgatatac gacggcggca tgatcacaac accgggcgcc
      541 acataagcgc cgcgtcgtac ggatgaaccg ccgggcacga gtcttatttt ttgtttcggc
      601 tgaaagcttc ggggaatcat cgaatcttta tccacaaatc cgccatagcg tccgtaggtg
      661 ggacgattca tggcgatcag cttaccttcg cgaaaagcgg atagtatggc ttctttgacg
      721 gcaacattag cccgccaaaa accgttatca tacgtcgcgg cccgtatctc tccgcgctcc
      781 agttgttcta atattttttt ccaatcgttt ttcatccgta ttgctccatc catcgttgca
      841 ctagactgtc cgccatgatt aatttatttc gatccggtaa atcttccata gaaagcggcg
      901 gtcgcacata cgccgaagtg atcaacccca attcgtgcaa taaagcttta gcaggtacag
      961 gattactggc caaaaacatc gcatcggaag ccgtacgcca tgtcggtaat aatacgccgg
     1021 tatcgcggtt cacacaacgg taaacaaatt cggcggacgc ttccggccac acattgctca
     1081 tcacagagac cagccccacg gctccggccg ctgcaaaaaa aggtgttaag ccgtcgtccc
     1141 cgctaaagaa tgcaacgccc ggtgcggctt cacggtaatc ctgaaattcg gtaacgctcc
     1201 cgctcgcctc tttcactgcc caaaatcgcg aatgatcctt aagttcacgc aggacggacg
     1261 tatgcaactt cacgcctgtc cgggatggca cattgtataa catacacggt ttttgagagg
     1321 cctcaagcag cgcagcaaac caactgcatt ggccgcgtgc accgggtttc gcatagagcg
     1381 gcgtgaccaa taaatacgca tggataaatg cgtgctgatt attctcttca atccaccgct
     1441 gggtggcgtc caaattgaat ccgccgacgc cgaccataac cgggacatgg agtttgcagg
     1501 ttccggtgaa gcggaccact tctctttttt catcatcgtt taacgccagg ccctcgcccg
     1561 tacttccgag tatcaccaca ccgtttccgg cggcttcttg acgacgcaat aaccgttcaa
     1621 aggataaaaa atcaattttt ccgttatcca aaaacggcgt cacgatcgct gtccatagtg
     1681 taaccgattg cagatttttc ataacttatt gtccttcgtt tagccaggtt tcaaacacat
     1741 cttccagagg aaaaataccg ctgggataat cctgcggccg cgatcgtttt tcgatgagct
     1801 tttgggccgt ccatatcgca ccgcgcgcaa atacggatcg gtcttcgaca tgatgtgaga
     1861 gcataagttt ttccgatgcg gtagccaaca caagcgaatg aaatccgaca acgtcacccg
     1921 tacgatcgct gtgaatgggt atttgttttc cgtaatgctg ccggatactg agcgcactgc
     1981 cgctgggttt gtctttttta tgtatgtgat gcacttcaaa tatctccgct tccgatccgg
     2041 cccattggct ttcaaggcgt ctgaattcgc gcgccatacg aaacatccat tgcataccca
     2101 tactaaaatt gctcgccgtt atccaagtgg cgccgctgtt ttctacagcg cgccgcacgt
     2161 ccattgtcca ttcaaatccg gtgctgccac acacgacggg aacggcggac tgaatgagta
     2221 ccggtaccag gtcattcaac acatggccgg gcacaaaaac gatcgccacg tccgaatttt
     2281 tcaacgtctc aaccgtcggt ggatttttgg aattaaaaac cgccgcgatt tcatccggag
     2341 gcaataagcg ttccacggcg ccgccggttt tcccgtttcc gatcaggctg attttaggca
     2401 tacgacctca ctttcgataa acgcacggtg aagtgcgcta acggcttgtg gcgcatccga
     2461 ctgatgcatc agaaagcaca tgttataaga actcgcaccc tgacaaatca aacgcacatt
     2521 aagaccggcg atcgcgtcaa aggtacgcgc cgcaataccg ggtgtttgtt gcagattgtt
     2581 tccgaccagc gcgatcagcg tcaggttttc ttcgatcgcg acatccgcaa accggcgaag
     2641 atcctggagc aactcttgtt tttctgaaat tttttcaccg gacgaaccca ggctcgtacc
     2701 gtctatcgta agggccacgc tgacttcgct ggtcgtcaca agatcaacgc tgagtttatg
     2761 atcggccagc acgccgaaca ttttggcaag aaaaccgtgc gtattgagca tgcgcaaact
     2821 gctcaccgtt ataagcgtct gatgcttgcg catcgcgatg gcacgtacca gcggcttatc
     2881 attgacatcc ggttcgatcc acgttccgcc ggcttcgggt tcaaatgtcg aacccacaaa
     2941 cacatgaatg cctttgcgaa tagctggcca caacgttgcc ggatgtaaaa ctttagcgcc
     3001 aaaattggcc aattcagcgg cttccgaaaa actgatttta ggaatagttt gcgcagccgg
     3061 caccaaattg ggatccatcg taaaaatccc cggtacatcc gtccaaatca aaacttcttt
     3121 agccgagagc gcctctccca ataatgccgc gctataatcg ctaccgccgc gcccgagcgt
     3181 ggtcgtacga ccgtcggtcg tagcaccgat aaaaccttgg gtcactataa cctgttcacc
     3241 gcacaaaggg agcaattttt tcaggcacgc agtttttata cgatccggtt gcggctcggc
     3301 tttgccaaat acctcatcgg taataataaa tgatcgggca tcggcttccg tgacattatt
     3361 tttgagatga aagagcattt tgccaaatat acgcgaggcc aagcgctcac cgaatgaaag
     3421 ccatgcatcg agccatactt cgcgattttc cgatgcagaa aataacgaca tgtgattttg
     3481 tgcggcaacg atcaattcat ctactgcaga agaatcaagt tccagtgcat ggcacatcgt
     3541 gcgatgccgg tcttcaattt tttttaacgc ctgatctgcc gacaccgtgt cgcatgcgca
     3601 tagatcgtga tatactttga gcagcgcatt cgtcgtaccg gatgtggcgc tcaccacgac
     3661 gacacgtatg cggttattgg caataacgat acgtgcgctt tggcgcatcg cttcggctgt
     3721 gcccatactg gtaccgccaa atttggcgat aatgagatcg ttcatgactc cattcctcag
     3781 gttataaata aaaaattcct tacaaacggt aaggaagagt catgatacgg gaattgtacg
     3841 ggattgagct taaggaaggc cttaatgcag atcgcgacgt caattccgga agcgcttcat
     3901 gcatcatgac agtcacgtgg gtttaacctc cgcaaccaac aaccgatttt ccagatccgt
     3961 tgtttcggcg tctatcaccg ccattcactg ctctagcgtg ccggatgcac tcgcaatgaa
     4021 tttttttgta gacgctcctc ttccgaaata tgtgatacat tggtaagata gaacgaaaaa
     4081 aaagaaatgc aacgggaaaa acgatgtgcc gaatttttaa aacgaattaa cctaaaacaa
     4141 aaatcttttt ttgccgaaca aaaaagttta tgcataaaaa aaaggctgca ctacctgtgc
     4201 agcctttgta tatctacggc ctatgcgtat aactaaaacg gtaaatcatc gctgcccgag
     4261 gagcctcccg catcggcggc atgatccata cctccgccca tatcggccgg atgcggaagc
     4321 gacgcttcgc cgcggcctcc gtccatctta gcgcccagca tttgcatgtt atcggcgcga
     4381 atttccgtta catatttttt ctgaccgtct ttgtcgtcat acgaacgtgt cttgatcttt
     4441 ccttccacgt aaacgagcga acctttttta agatattggc cgcaaatttc ggccaattta
     4501 ttccacgcca cgatccgatg ccaatccgtg gattcgatca gcttaccttc tttatctttg
     4561 taggattcgt tggtagcgac attaaacgtc gcaaccggcg caccgctcgg tgtatatcgc
     4621 agttccggat caccgcccag acggccgatg atgatgactt tattgagtgt tccttttgac
     4681 ataagtatat ccttactctt ctatcgtttt attttgattt tttttcggat gcggtcggcg
     4741 caggcttcgg cgattctttt tccgtcgccg ccggtgcgat acccaattcc tgacgcacgc
     4801 gggtttcgat ttttttcaag acgaccggat tatcggtcag atattgtttc gcattctcgc
     4861 ggccttggcc gattttttca tcttcaaaag aaaaccatgt tccgcttttt tggacgatat
     4921 tcatcgtgga tgccaagtca accaaatcgc cttcgcgcga aatgccggta ccgtacatca
     4981 gatccacttc gacttctttg aaaggcggcg ccagtttatt tttcaccact ttgactttgg
     5041 tgcggttacc gacaagattt tcaccggctt tgatttgtcc gatgcgacgg atgtccacac
     5101 gaatggatgt ataaaacttc agggcccgtc cgccggtcgt cgtttcggga ttgccgaaca
     5161 tgacgccgat cttatcgcgc acctgattga taaaaataac gcaggttttg gatttgctca
     5221 ccgcgccggt cagtttacgc atggcctggc tcatgaggcg cgcctgcaca cccatggtcg
     5281 catcgcccat ttcgccgtca atttcactgc ggggaacaag ggcggcaacg gagtcaatca
     5341 cgatcacatc gacggcattg ctgcgaacca gacgttcggt aatttccagc gcttgttcgc
     5401 cgttatcggg ctgagaaact aaaagattat caatatccac gccaaggcgc ttggcatata
     5461 tcgcatcgag tgcgtgctcg gcgtcgataa atgctgccac gccgccgact ttttgcgctt
     5521 cggctatgac atgcagcgac agagtggttt taccggaaga ttccggcccg taaatttccg
     5581 taatacgtcc gcggggaata ccgccgacgc ccagtgcggc atcgatcgaa atggaaccgg
     5641 tggaaaacac ctcgacctgc atggccgtgc gctcgcccag tttcatgata gatcccttac
     5701 cgtattcttt gacaatgtct gtcagcgtgc ggtccaacgc ggcttttttg tctttgtccg
     5761 acaataccat ttcgtctgcc atcctaagct ccttgtttcg tgtgtgtctg tagttgtgct
     5821 ttgttattta cgtgcgcaaa gtataaaaac gcttttttac gtgcaaataa aattccgtgc
     5881 aaaacatcgt actgtcaaag cttgccggtt catggatacc gacaagctct ggcgtgcaca
     5941 aaacggatgt catacggtgg cttcggcaag aatttccatt tcttcctgta caccttccga
     6001 ctccgacgac ttggcgttct tatgccccag catctgcatc gtttcggcga cgacttccgt
     6061 caacgcatgt ttacccccgc tcttgtcatc ccaccgacgt gttttcatcg
//
LOCUS       DAOQNQ010000195         6107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_5035_length_6107_cov_5.694481, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000195 DAOQNQ010000000
VERSION     DAOQNQ010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5035_length_6107_cov_5.694481"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="PK754_11325"
     CDS             <1..1219
                     /locus_tag="PK754_11325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNB10139.1"
                     /translation="GHIVPRDDKENYLSRAGSPTQSSAYAAQFWRKRDPLLSSDYNER
                     MLEHYNRMAYVNLRFGIPRQNIMGWETERGMIYIRYGKPSQWVRTQPDEKMLGGTEIW
                     YYPRFSFRFEDHYSSGKFLLDNPSLLASESAYNEHSDDYVFPAERLFPVSARVYQFKS
                     QSGLNKIRVYYSALIKSMDPDYDTYGPTGKTQIGFFITDSERNILSKTMDTSLVEPKE
                     TRDAYVYKAATMETFIQSKEPVKWSLELKSLGEFRYAMAGDSLTLRSFSSRTLDMSDV
                     VLADYVDENKPYAVFANTQGVLQADKPLYFFAEVYNLTLEKDQSRFVVEAGLAPHATG
                     GIRTFLQKIFGNTDALVYSTFEQTGTSRDDTVTFALNVSEVPPGNYDAVVRIRDLISG
                     HTTEQKTSIRIVR"
     gene            1216..1494
                     /locus_tag="PK754_11330"
     CDS             1216..1494
                     /locus_tag="PK754_11330"
                     /inference="COORDINATES: protein motif:HMM:NF017574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNB10140.1"
                     /translation="MKWLGWLHKPKSAISVEIFSKSDCHLCDEMSDAFHGLQNEFPMT
                     IKYTDITSDEALFEKYRYDIPVVFVNGRKAFKHRATTDAIRKRLRREI"
     gene            complement(1496..2464)
                     /locus_tag="PK754_11335"
     CDS             complement(1496..2464)
                     /locus_tag="PK754_11335"
                     /inference="COORDINATES: protein motif:HMM:NF036967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HNB10141.1"
                     /translation="MLTKLARTIKDYNDSGFSSTINNQGKRLMNADGRFNVIKRGASF
                     ADRFSYLNALLTMSWGRFFAVIFGIYTAINLVFASVYFVIGREHFSGLLSSDTASEYI
                     EDFFFSAQTLTTVGYGRVNPTGVAANIVASAEALIGLLCFALATGLLYARFSRPDAKI
                     VWSDKVLIAPYRDGQAVMMRLANSKDTQLMDVEVNMMFSMVLDESGTPTRKFFNLNLE
                     RSTVNFLPLNWTLVHPLNTESPLYGLTPADLMQGEAELIVSLKGTEETSMQIASARRS
                     YLHADFEWNARFLLMFAPSPDGTATVLHLDKVGIFERLHNVKETAA"
     gene            complement(2558..3901)
                     /locus_tag="PK754_11340"
     CDS             complement(2558..3901)
                     /locus_tag="PK754_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNB10142.1"
                     /translation="MPNSKTKPETKLFILDTNVILYDYTCIYNFKEHDVVIPIVVLEE
                     IDQFKKGSDLINFQAREFIREMDKLSKAQLFNKGLQLKKGHGRLFVEVGEAHSELVYN
                     AFTTNKPDHRILALADQLRHKRTDREVIIISKDINLRMKAKSLGIPAEDYETGKIRNV
                     DELYTGIQTHENFNTEVIRRFYEQTEAVPADDLKLPRKPVANEYYILKNHSSSVLTHY
                     NPSAQGLERIEKKRAYGIEPRNAEQVFALDALMRPEVAVVTLTGKAGTGKTLLALAAA
                     LQQRQLYSQIYLARPVVPLSNRDIGYLPGDVHEKLDPYMQPLWDNLAVIKNKFSQEST
                     EVTRIAEMLEREKLVIAPLAYIRGRSLDKIFFIVDEAQNLTPHEIKTIITRAGEGAKM
                     VFVGDIYQIDSPYLDTQSNGLAYLVDRMKGQDLFAHVNLLKGERSALAELASNLL"
     gene            complement(3914..4831)
                     /locus_tag="PK754_11345"
     CDS             complement(3914..4831)
                     /locus_tag="PK754_11345"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNB10143.1"
                     /translation="MDSTSVNAWNSHASAIPTLYALLVVRRSGLVFEAYYQSTDSTTF
                     FAQRSVTKSIIGALTGVALYDHTIHNVSDKLSRYFPKYYEDISDNRARQITLKHLLTM
                     SAGYAWNEVADRLYGNYVSFAVHLPMDTVPGRVFNYSSAVPHIISGILSDRTDNKLIS
                     WSRNKFFSKLGIDSMHWQADPQGIPLGGTGLYLRARDMAKIGMTYLDGGCYDGQRVFS
                     DLWPDISFTDALDDNHAYGHLWWLDEAMDYPAAYAFGYGGQLIYVVPALDIVVIATAD
                     PNVNYTVSDQVLTSVRQIIHNDILPSVIP"
     gene            complement(5151..>6107)
                     /locus_tag="PK754_11350"
     CDS             complement(5151..>6107)
                     /locus_tag="PK754_11350"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922757.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HNB10144.1"
                     /translation="VFDCANKCGIGKRFIDPMGHIKMMSAAQPFLSGAISKTVNIPNE
                     ASVKDIEKIYFEGWKLGLKAVALYRDGSKFSQPLSSKGISTESEAGQSAEEVKAQIDA
                     AVKAAIEHAAKELRRGQKKPMPLKRNGFTQEVELGGHKIYFRTGEYEDGQIGEVFIDM
                     FKEGAAYRSLLNCFAVAVSMGLQYGVPLEKFVEKFTFTRFEPSGFTNHPNIKNATSII
                     DFIFRVLAMEYLGRMDFVHVPPQIDTATAPVAQSGTNETPTASHSHTHDTHAHDASAN
                     PMEKQLSEMMGDAPACDVCGHMTVRNGACYKCLNCGNSMGCS"
BASE COUNT         1634 a         1505 c         1357 g         1611 t
ORIGIN      
        1 cggacatatc gtaccccgag acgataaaga aaactatttg tcccgcgccg gaagccccac
       61 gcaatcatca gcttatgccg cacagttttg gcggaagcgc gatcccctgt tgtcgtcgga
      121 ctacaatgag cgtatgttgg agcattataa ccgtatggcg tacgtcaatc ttcgttttgg
      181 aataccgcga cagaatatca tgggttggga aacagaacgg ggtatgatct acattcggta
      241 cggcaaaccc tcgcaatggg tgcgcacgca accggacgaa aaaatgctgg gcggtaccga
      301 aatatggtat tatccgaggt tctcatttcg ttttgaggat cattacagca gcggaaaatt
      361 tttgctggat aatccctcac tgctcgcatc ggaatcggcc tataacgagc attcggacga
      421 ttacgtattt ccggccgaac gtttattccc tgtgtcagcc agagtgtacc aattcaaatc
      481 ccaatccggg cttaacaaaa tacgcgtgta ttattctgcc ctgatcaagt ccatggatcc
      541 cgattacgac acgtatggtc cgaccggcaa aacacaaatc ggttttttta tcacagattc
      601 cgagcgaaac attttatcaa aaaccatgga tacttcgctc gtcgagccaa aagaaacccg
      661 ggatgcgtat gtatacaaag cggccacgat ggagaccttc atacaaagta aagaacccgt
      721 gaagtggtct ctcgaattaa aatctttggg tgaatttcgt tatgcgatgg ccggcgattc
      781 gttgaccctc cgaagttttt cgtcacgcac tttggatatg agcgatgttg ttttggccga
      841 ttatgttgac gaaaataaac cttatgccgt ttttgccaat acccaaggcg ttttgcaggc
      901 agacaaaccc ctatactttt ttgccgaagt ctacaacctt actttagaaa aagatcaaag
      961 ccgttttgtc gtggaagccg gattggcgcc gcatgcgacg ggtggcatac gaaccttttt
     1021 gcaaaaaatt ttcggaaaca ccgatgcctt ggtgtattcg acgtttgaac aaaccggcac
     1081 gtcccgtgat gataccgtta cctttgcgct caatgtcagt gaggtaccgc cgggaaacta
     1141 tgatgccgta gtgcgtataa gggatttgat ttccggacat accaccgaac agaaaacatc
     1201 catcaggatc gtacgatgaa atggttagga tggcttcata aacccaaatc agctatttcc
     1261 gttgaaattt ttagcaaatc ggattgtcat ttatgcgatg agatgtccga tgcatttcat
     1321 ggtctgcaaa atgaattccc catgaccata aaatacaccg atattacttc cgatgaagcc
     1381 ttgtttgaaa aataccgcta cgatattccc gtagtatttg tcaatggtcg caaagcattc
     1441 aagcatcgtg caacaaccga tgcgatacga aaacgtttgc gcagagagat ataaactaag
     1501 ccgcggtctc ttttacgtta tgcaagcgtt caaaaatccc cactttatcc aaatggagca
     1561 ccgttgccgt tccatccggt gatggcgcaa acatcagcaa aaaacgcgca ttccattcga
     1621 agtcggcatg caaataagaa cgccgtgcac tcgcaatttg catggaagtt tcttccgtgc
     1681 ctttgagcga tacgatgagt tcggcctcac cttgcatcaa atcggcaggc gtcaaaccat
     1741 acaaaggact ttcagtgttc aacggatgta ctaaagtcca gttgagcgga agaaagttga
     1801 ccgtggaacg ctccaaattc agattaaaaa atttacgcgt cggcgttccc gattcatcca
     1861 ataccattga aaacatcata ttgacttcga catccatcag ctgtgtgtct ttggaattgg
     1921 ccagccgcat catgaccgcc tgtccgtcgc gatacggagc gatcaaaact ttatcgctcc
     1981 aaacgatttt ggcatccggt ctggaaaaac gagcataaag taagccggtc gcaagagcaa
     2041 aacagagtag cccgatcaat gcctccgccg aagcgacaat attggccgca acgcctgtgg
     2101 gattgacgcg cccgtaaccc actgtcgtaa gagtctgtgc gctaaagaaa aaatcttcga
     2161 tatattcact ggctgtatca gaggacaata agcctgaaaa atgttcccgg ccgatcacaa
     2221 aatataccga tgcaaacaca aggttaatcg cagtatagat accaaagata acggcaaaaa
     2281 atcgccccca actcatagtt agtaacgcat tgagataact aaaacgatcc gcaaaggatg
     2341 cgccacgttt gatcacatta aatcgcccgt cggcattcat cagacgtttg ccctgattat
     2401 tgatcgtgct gctaaaaccg gaatcgttgt aatctttgat cgtgcgcgca agtttcgtga
     2461 gcataggttt ttctgccaat tcgtaggtga attaaaaagt atttttttat aaatttttga
     2521 ctgcaatgac ggtcgctaat cggttaccga cgaaccatta taacagatta ctggccaatt
     2581 ctgccaaggc gctgcgctcc cctttgagca aattgacatg cgcaaacaga tcctgacctt
     2641 tcatgcgatc taccagatac gccaaaccat tggactgtgt atcaagatac ggtgaatcaa
     2701 tttgataaat atcgccgaca aataccattt tagcaccttc gcccgcacgg gtgataatgg
     2761 tttttatttc atgcggcgtc aggttttgcg cttcatctac gataaaaaat attttatcca
     2821 ggctccgccc gcggatataa gccagcggcg caatgaccaa tttttcgcgc tcaagcattt
     2881 cagcaatgcg tgtcacttcg gtgctttcct gtgaaaattt attttttatc acggccagat
     2941 tatcccacag cggttgcata tacggatcaa gtttttcatg gacatcgccc ggcaggtatc
     3001 cgatatcccg attactgagc ggtacgaccg gtcgtgctaa gtatatttga ctgtaaagct
     3061 gtctttgctg cagtgcggcg gccagtgcca gcagcgtttt gcccgtaccg gctttacccg
     3121 tcagcgtaac caccgcaacc tccgggcgca tgagcgcatc caatgcaaac acctgctccg
     3181 cattgcgggg ttcgatgcca tacgctcgtt ttttctcgat acgttccaaa ccctgcgccg
     3241 atggattata gtgtgtgagt acgctgctgc tgtgattttt gagaatatag tattcattag
     3301 ctaccggttt acgcggcagt ttgagatcat cggccgggac ggcctcggtc tgctcataaa
     3361 atctgcggat cacttcggta ttaaaatttt catgcgtctg aatgcccgtg tacagttcgt
     3421 ccacgttgcg aattttcccg gtctcatagt cctccgccgg aatgccgagc gatttggctt
     3481 tcatccggag attgatgtct ttgctgatga tgatgacttc gcgatccgta cgcttatgcc
     3541 ttagctgatc cgccagcgcc aaaattcggt gatcgggttt attggtcgta aaggcattat
     3601 acacgagctc cgaatgcgcc tcaccaactt cgacaaataa acgcccgtgg ccttttttta
     3661 attgaaggcc tttattgaat aattgtgcct tggataactt atccatctcg cggataaatt
     3721 cgcgcgcctg aaaattgatc aaatcactgc cttttttgaa ttgatcaatt tcttccagta
     3781 cgacgatggg tatcacgaca tcatgctctt taaaattata aatgcacgtg tagtcataca
     3841 ggatcacatt ggtgtcgagg ataaaaagtt tcgtttcggg cttggtttta gaattcggca
     3901 taggcttcct tggttaaggg atcacggaag gaagaatatc gttgtgaatt atttggcgaa
     3961 cggatgtcag gacttgatcg gatacggtat agttgacatt agggtcggcg gttgcgatca
     4021 ccacgatatc cagcgccggt acgacgtaga tcagctgtcc gccataaccg aaggcataag
     4081 ccgccggata atccatagcc tcatccaacc accacagatg gccatacgca tggttatcat
     4141 ccaaagcatc ggtaaaagaa atatccggcc ataaatctga aaaaacacgc tgcccgtcgt
     4201 aacaaccacc atccagatac gtcataccga tcttggccat atcccgtgcc cgcagatata
     4261 aacccgtacc gcccaaagga atcccctgcg gatcggcctg ccaatgcatc gaatctattc
     4321 caagcttcga aaaaaattta ttcctagacc atgatattaa tttattatcg gtgcgatctg
     4381 acaatatacc cgatataata tgcggcacgg ccgaactgta gttaaaaaca cgccccggca
     4441 cggtatccat cgggaggtgt actgcgaatg aaacataatt cccatacaaa cgatcggcga
     4501 cttcattcca cgcatatccc gcagacatcg tcaggaggtg cttcaatgtg atttgccgcg
     4561 cacggttatc actgatatct tcataatatt ttgggaaata ccggctgagt ttatcggaaa
     4621 cattgtggat ggtatgatca tacagcgcaa ctccggtcaa agcgccgatg atgcttttgg
     4681 taaccgaacg ttgtgcaaaa aacgttgttg aatcggtgga ctgatagtag gcttcaaaaa
     4741 ccaaaccgct tctccgcaca actaataagg cgtatagtgt tgggatcgcg gatgcatgac
     4801 tgttccacgc attgacgctt gtagaatcca ttctcgcttc cgcaaaagat atacggcgca
     4861 aagcggccgt ttcatcgcac ggcggttcct gcaggtcatt gctacgacag gaggtaaaaa
     4921 gtaataccgt tagaatgatt tttttttgga tcattatttg tatagaaaca tccgtatcac
     4981 tgaataatcc tattataaaa aaagcacgac aatacatccg ttaccggatc atcatcgtgc
     5041 tctctattca taactcaagg acagtcagca ttcagtagat tcgacctttt ctttatagtc
     5101 ggtcacttcc caaaaaacgc tgcaattttt ttcaaaacca tacgcccgga ttacgagcag
     5161 cccatactgt taccgcagtt cagacacttg tagcaagctc cgttacgaac cgtcatatgc
     5221 ccgcatacat cgcaagccgg cgcatcaccc atcatttcgc tcagttgttt ttccatcgga
     5281 ttggctgagg catcatgcgc atgggtatca tgcgtatggg aatgtgaagc cgtcggcgtt
     5341 tcgtttgtcc ccgattgggc aaccggtgcc gtagccgtat caatctgcgg cggcacatgt
     5401 acgaagtcca tacgtccgag gtactccatc gccaatacac ggaaaatgaa gtctatgatg
     5461 gatgtcgcat ttttaatatt gggatggttt gtaaaaccgc tcggttcaaa ccgtgtgaac
     5521 gtaaacttct ctacaaattt ttcaagcggc acaccgtact gcaaacccat ggagacggcc
     5581 acggcaaaac agtttaacaa actgcgataa gccgctcctt ctttaaacat atcgataaat
     5641 acttcaccga tctgaccgtc ttcgtattca cccgtacgga aataaatctt gtgaccgcca
     5701 agctcaacct cttgggtaaa cccgttacgt ttgagcggca tcggtttttt ctggccccgg
     5761 cgcaattctt tggccgcatg ttcgatcgca gctttgaccg cagcatcaat ttgggctttc
     5821 acttcttccg cactttgtcc ggcctcagac tccgtcgaaa tgcctttgct gctcagcggt
     5881 tgggaaaact tggatccgtc gcgatacagc gctacggctt tcagcccgag cttccagcct
     5941 tcgaagtaga ttttctcgat atctttgacg ctggcttcgt taggaatatt caccgtcttg
     6001 gaaattgctc cggacaggaa aggttgcgcg gcggacatca tcttgatatg tcccatcggg
     6061 tcaataaacc gtttaccgat accgcattta ttggcacaat caaatac
//
LOCUS       DAOQNQ010000196         6069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_5086_length_6069_cov_4.728633, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000196 DAOQNQ010000000
VERSION     DAOQNQ010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5086_length_6069_cov_4.728633"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..2323
                     /locus_tag="PK754_11355"
     CDS             26..2323
                     /locus_tag="PK754_11355"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB10145.1"
                     /translation="MIYRLSLSGLFLLVGIFSLSAQVTFTLDGTVKDLYTGRPMPSVN
                     VYVQEIQKGTLTDTAGYFAIKNLKKGYYTLVLTRVGYKRVTRKVQLFKDINFPIKMEE
                     QAIVFEAQEITPGVTELSIDEGASSTVSTQEIVNSVQVFSKDVYRALQTVPGISNSEW
                     SSKPHVKGGNPDETAVIIDNLEIREPFHLEEIDGPYSVISSDLVKSMKVLTGGFAPKY
                     GDRMSGLLFINTIDRIEDDSMKISVDFSNATASLNQRINDRLTVFASGRKSYIFLLEN
                     AIDNNFPTTIWDGWVKADYKVDPKNRITVNYLMLNDNIQYKESDSSEFFFSTKTNYYL
                     WLNWLNTVSTDRYSTTTLGFQDLTKKSDFLFNSSYTDDNQDNRTTKLLTLKHDHSWRF
                     NRQNTIEFGAEFNQFWSEYYFREFRLNPTETTPILVSQDIIFVDSKFSGYTGAAYFQN
                     SYKINDKMTVLGGLRATGQSYANAIQLAPRAAMSYDFNSDLTLRLAYGWYYQPDNFQK
                     LRSYANQHVPNITPEKSIHYIASGIYKYDDNTNITLDAYYKDYPKLFDDFTFDFSNRI
                     EGVGLVDKPYQTVSGFATGFDLLARHRYGNSNLFSLAYSLSTSHIRNERKLETYRDLD
                     RTHSLLLSNISNLPSRMALSSSFRIRSGDPFTPSTVRILGDGTVENSKIYYTTQTKNS
                     GRLPIFYTLDLRLEKRWTGQQMHWVTYVSVVNVTDHKNVRQRSWKRHINPDGTLAPFT
                     QEDQLYFPRLFMLGVSAEINLPRTR"
     gene            2332..3468
                     /locus_tag="PK754_11360"
     CDS             2332..3468
                     /locus_tag="PK754_11360"
                     /inference="COORDINATES: protein motif:HMM:NF028473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10146.1"
                     /translation="MRFLKLSFLIAVIACENWPENKSQEFFDFGKEEYGVSAIPYSDG
                     GMIIGGTTTSIRGNQDMLLMRLDDSHQKLWVKAIGGNAIDRCHDVFELSDHSVVAVGS
                     VQKSVGNNDMLIVRLSKYGEIIWSKNIGGIYNEEARAVVQANDGNIIVAGTTASFGAG
                     SNDVFLVKMNLAGALLWQKTYGDQKVSGAYDIQLTKDKGFIISGFMANGITPYDAMLI
                     KISESGEEQWTRTYPKSGKANAKRVIELSDQSFLVTGIAQYSDVPGSNSSFVFHADAT
                     GNIIWYQNYNIFVNNESFDMVVLNEQNAVVTGFIDGANTQLYALTVNLSDGIPNGFKR
                     YLDGATSWGYSIIKTENKFWAIGGVIDSDNRTTDVAVIEITLPL"
     gene            3548..4621
                     /locus_tag="PK754_11365"
     CDS             3548..4621
                     /locus_tag="PK754_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10147.1"
                     /translation="MPDSREIQTFDFGENDYAISASILQDKGMIIGGSTNSIRDNRDM
                     LVMRIDENNQKVWVKSIGGKTTDRCFNVIELDDHSFIAVGSIQVSSGNLDMLVLRLSK
                     TGEIIWSRNFGGRYNEDGRAVVQADDGSVIVVGTTASFGAGSNDVFLVKITKEGQLFW
                     QRTFGNTSIDVGNDIKKLENGDFIIAGYSSNGIYPFHTLLIRVSSEGDELWKKIYTMG
                     VSSDGRSVSILPDGGYIVSGNVRLSPYSGAISTMILRTDADGNVMWHNSFLGSTETNH
                     GIILKDNIYAVAGQITTRQTNAFVMTVDAISGAQIDFKMYGTSGYSSANAILKKGSDF
                     FVVGWIETDRIKKIDVFIEKINF"
     gene            complement(4688..>6069)
                     /locus_tag="PK754_11370"
     CDS             complement(4688..>6069)
                     /locus_tag="PK754_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNB10148.1"
                     /translation="TLDAMRWGGIYDHIGFGFHRYSTDARWFLPHFEKMLYDQALTAI
                     AYTEAYQITGYARFKETAQNILEYVLRDMEAPSGGFYSAEDADSDGEEGKFYLWSFSE
                     IAAILGTIDAELFAKVYNLDREGNYYDEATREPNGSNIIFLTKEWSDITAETGITEHA
                     LREQTDQMRKKLLEVRSRRTRPLKDDKILTDWNGLMITALCKAAQVFEDATYVHAAMR
                     AEHFIRTRMMTKDGHLYKRHRLDESGLPAHLDDYAFWIWGLIELVETTGDVQFLKEAR
                     RLTTIVVDEFEDPDNGGFYFTSQNGEPLIRRTKELYDGAIPSGNSVMLSNLYRMSRLL
                     HEPALAGKAERIGLSFADSIHRMPMAFAQFLSGWDRLQQAPMEIIITGTKDNATFQVM
                     STLAHHAYHANKTLIIKSSDVWDDLSPQYKNYPINSDYAKAYVCRDFHCEAPVSDKTD
                     LQNKLRNAP"
BASE COUNT         1753 a         1149 c         1370 g         1797 t
ORIGIN      
        1 taatgtcata acaacggagg tacgcatgat ctatcgttta agtctttcgg ggttgtttct
       61 gcttgtgggt attttttctc tttcagcgca agtgacgttt acattggacg gaacggtcaa
      121 agatctctac acggggcgtc ctatgccatc ggtcaatgtg tacgttcagg aaattcaaaa
      181 aggcactctc acagacacag ccggatattt tgcaatcaaa aatcttaaga aaggttatta
      241 cacgcttgtg ctcacgcgcg tgggctataa gcgtgtgacc cgcaaagtgc agcttttcaa
      301 agatattaat ttccccatta agatggagga gcaagcgatc gttttcgaag cgcaggagat
      361 cacgcccggc gtgacggaat tatctataga cgaaggcgct tcttccaccg tttccacgca
      421 ggagattgtt aactctgttc aagtattcag caaagatgtg taccgtgcat tgcagacggt
      481 ccccggtatt tccaatagcg aatggagctc taaaccgcac gtcaaaggag gaaatcctga
      541 tgagacggcg gttatcatcg ataatctcga aattcgtgaa ccttttcacc ttgaagaaat
      601 tgacgggcct tatagtgtca taagttccga tcttgtgaaa agtatgaagg tcctaaccgg
      661 aggttttgca ccgaagtacg gtgatcgtat gtcggggctg ttgtttatta atactattga
      721 tcgcattgaa gatgattcga tgaaaataag cgtggatttt agtaatgcaa cagcatcgct
      781 taatcaacgc atcaacgatc ggttaacggt gtttgccagt gggcgaaaga gttatatttt
      841 cttactcgaa aatgcgattg ataataattt cccgactacg atttgggatg gatgggttaa
      901 agctgattac aaagtagatc ctaaaaaccg gattactgta aactatctta tgctgaatga
      961 taatattcag tataaagaaa gcgattcgag tgagtttttc tttagtacaa aaacgaatta
     1021 ttacctttgg ctcaattggc tcaatacggt ctcaacggat cgctatagta caaccacatt
     1081 gggctttcag gatttgacaa aaaaatcaga ctttcttttt aacagcagct ataccgatga
     1141 taatcaagat aaccgtacga caaaattatt aacactcaag cacgatcact catggagatt
     1201 taacagacaa aacacaatag agttcggcgc ggaatttaat cagttttgga gtgaatatta
     1261 tttccgcgaa tttcgactca atcctacaga gacaacgcct attttggttt cccaggatat
     1321 tattttcgtt gatagcaaat tcagcggcta taccggagca gcttattttc aaaattcata
     1381 taaaattaat gataaaatga ctgttttagg cggtttacgc gctacgggtc aaagttatgc
     1441 taatgctatt caattggctc caagagcggc catgagctat gattttaatt cggaccttac
     1501 tttacgactt gcgtatggtt ggtattatca acccgataat tttcaaaaat tacgttcgta
     1561 tgctaatcaa cacgtcccaa atattacacc ggaaaaaagt attcattaca tcgctagcgg
     1621 tatttataaa tatgatgata ataccaacat tacgttggat gcgtattata aagattatcc
     1681 aaagcttttt gatgatttta catttgattt ttcaaatcgt attgaaggcg tggggcttgt
     1741 tgataaaccc taccagacag tatccggttt cgcaacgggt tttgatcttt tggcgcgtca
     1801 taggtatggt aattctaact tgttttctct ggcgtactca ttgagtacaa gccatattcg
     1861 taatgaaaga aaacttgaga cctatcgcga tcttgatcga acacattcgt tattgctcag
     1921 caatatttcc aaccttcctt cacgaatggc tttatcgagt tcatttcgga ttcgatccgg
     1981 cgatccattt acgccgagta cagttcgcat tttgggtgat gggacagtag aaaacagtaa
     2041 aatttattat acgacgcaaa ccaaaaatag cggacggtta ccgatttttt acacgctgga
     2101 tttacgtctc gaaaagcgct ggacggggca gcagatgcat tgggtcactt acgtcagcgt
     2161 agtcaacgtg accgatcata agaatgttcg gcagcgttca tggaaacgtc atatcaaccc
     2221 ggatggaaca ttagcgcctt ttactcaaga ggaccagctt tatttcccaa gattattcat
     2281 gttgggagta agtgctgaaa ttaatttgcc aagaacacgt tgaggtgtaa aatgagattc
     2341 ctaaaattat catttcttat tgctgtaata gcttgtgaga attggcctga aaataaatcc
     2401 caagaatttt tcgattttgg taaagaagaa tatggtgtca gcgcgattcc gtacagcgat
     2461 ggcggtatga tcattggcgg tacaacgaca agtatccgcg gaaatcagga tatgctctta
     2521 atgcgattgg atgattctca ccaaaaactg tgggttaaag ctattggcgg caatgctatt
     2581 gatcgatgcc atgatgtctt tgaactttcg gaccactcgg tagtggctgt aggttcagtg
     2641 cagaaatccg tagggaataa cgatatgctg attgtgcggt taagcaaata tggcgaaatt
     2701 atatggtcaa agaatatcgg cgggatttat aatgaagaag cacgagccgt agtacaagca
     2761 aatgatggta atatcatagt ggccggtaca accgcgagtt ttggggccgg tagtaatgat
     2821 gtattcttgg tcaaaatgaa tttagccgga gctttactat ggcaaaaaac gtatggcgat
     2881 caaaaggtaa gcggtgcgta cgatattcaa ttaactaaag ataaaggatt tattatatcc
     2941 ggtttcatgg caaatggcat aacgccatac gacgcgatgt tgatcaaaat ttcagaaagt
     3001 ggagaggaac aatggacgag gacatatccc aagagcggta aagcgaatgc taaacgcgtt
     3061 atagaattgt ccgatcagag ttttcttgtg acaggtatag cgcaatatag cgatgttccc
     3121 ggtagtaatt cctcatttgt atttcatgca gatgccactg gtaatattat ctggtaccaa
     3181 aattataata tttttgttaa taatgaatca tttgacatgg ttgtgttaaa tgaacaaaac
     3241 gccgtagtaa ccggatttat tgacggagcg aacacacaat tatatgcttt aacagtgaat
     3301 ttgtccgatg gtattccaaa tggttttaaa agatacttgg atggcgctac aagttggggt
     3361 tattcaatca taaaaactga aaataaattt tgggcaatag gaggtgttat tgatagcgat
     3421 aaccggacca cggatgtggc cgttattgaa ataacattgc ctttatgagt aaagtatagg
     3481 agatcacatg agattattca tgctttatgt atgtacttgt attttcatgt tttgcagttg
     3541 tgaaaatatg cctgattccc gcgaaattca gaccttcgat tttggagaga atgattacgc
     3601 catatcggcg agtatcttgc aggataaggg catgatcatc ggaggaagca ccaatagtat
     3661 tcgcgataac cgggatatgc ttgttatgcg catcgacgaa aataatcaaa aagtttgggt
     3721 taaatcgatc ggaggtaaaa cgactgatcg ctgttttaat gttattgaac ttgatgatca
     3781 ttcatttata gcggtcgggt caattcaggt ttcgagcgga aatttagata tgttggtttt
     3841 gcgtttgagt aaaaccggcg aaataatatg gtcgcgcaat ttcggcggtc gctataatga
     3901 agatggtcgc gccgtagttc aagcagatga cggctctgta atagtggttg gaacgacagc
     3961 aagttttggc gccggaagta atgatgtttt tttagtaaaa attacaaaag aaggtcaact
     4021 tttttggcaa cgcacatttg gtaatacaag tatagatgta ggtaacgata ttaagaagct
     4081 tgaaaacggt gattttatta ttgcaggtta ttcatccaat ggtatttatc cttttcacac
     4141 gttattaatt cgggtaagtt cggaagggga tgagctttgg aaaaaaatat ataccatggg
     4201 cgtatcatcg gatggaaggt cggtaagtat attgccggat ggcggttata tcgtttcggg
     4261 caatgttcgg cttagtcctt attcaggtgc tatttctacc atgatattgc gtacggatgc
     4321 cgatggtaat gtgatgtggc acaatagctt tttgggaagt acagaaacca atcacggtat
     4381 catcctcaaa gataatatct atgccgtagc gggacaaata acaacgcgtc agactaatgc
     4441 gtttgtaatg accgttgacg cgattagcgg agcgcaaata gattttaaaa tgtatggcac
     4501 gtcgggctat agtagcgcaa atgctatact caagaaaggt tctgactttt ttgtcgttgg
     4561 ttggatagaa actgacagaa taaaaaaaat agacgtattc attgaaaaaa taaatttcta
     4621 aaaccctacg ttccttcgct ttcgcgtcta tttactggca ttgtgcaatt ggttaatgac
     4681 atgaaaatca cggtgcgttg cgtaatttat tttgaagatc ggttttatcc gacaccggtg
     4741 cctcacaatg aaaatcgcgg catacatagg ctttagcata atctgaattg atcggataat
     4801 ttttatactg tggtgacaaa tcatcccaga catccgatga tttgataatt aaagttttgt
     4861 tcgcatggta tgcgtgatgt gccaaagttg acatgacctg aaatgttgcg ttgtcttttg
     4921 ttcccgtgat aatgatctcc atcggcgctt gctgcagtcg atcccatcca ctcaaaaatt
     4981 gtgcgaacgc cataggcatg cgatgaatgc tatcggcgaa ggataaaccg attcgttccg
     5041 cttttccggc taaggccggt tcgtgtagca atcggctcat gcggtagagg ttcgataaca
     5101 ttacggaatt accggaaggt atcgctccgt cgtatagttc tttagtacgg cggatcaagg
     5161 gctcaccgtt ttgcgaggta aaataaaacc cgccgttgtc cggatcttca aattcgtcaa
     5221 ccacgatggt tgttaatcgt cgcgcttctt ttagaaattg tacgtcgcct gtagtttcta
     5281 caagttcaat caatccccat atccaaaatg cgtagtcgtc caaatgagcg ggtaatccgg
     5341 attcatccaa acgatgacgt ttatacaggt ggccgtcctt cgtcatcata cgcgtacgga
     5401 taaagtgttc ggcacgcatc gcggcatgca cataagttgc gtcttcaaaa acctgagcgg
     5461 ccttgcataa tgcggtgatc atcaagccat tccaatccgt aagaatctta tcgtctttta
     5521 aaggccgcgt ccggcgtgag cgtacttcaa gcagcttttt tcgcatctgg tctgtttgtt
     5581 cacgcaacgc atgttcagta attccggttt cggccgtgat atctgaccat tctttggtca
     5641 aaaatatgat gttcgaccca ttgggctcgc gtgtggcttc atcataataa ttaccttcac
     5701 gatccaggtt gtataccttt gcaaataatt cagcgtctat agttccaagt atggccgcga
     5761 tttcactgaa cgaccataaa taaaattttc cctcttcgcc atcgctgtcg gcatcttcgg
     5821 cggaataaaa cccgccgctc ggcgcttcca tgtctcgtag tacatactcc aaaatattct
     5881 gcgctgtttc tttaaatcga gcgtatccgg ttatttgata ggcttcggtg taggcgatag
     5941 ccgttaaagc ctgatcgtag agcatttttt caaaatgcgg taaaaaccat cgtgcatcgg
     6001 tggagtagcg atgaaatcca aatccgatat gatcataaat accgccccat cgcatagcgt
     6061 ccaatgttc
//
LOCUS       DAOQNQ010000197         6050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_5112_length_6050_cov_7.075063, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000197 DAOQNQ010000000
VERSION     DAOQNQ010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5112_length_6050_cov_7.075063"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..574)
                     /locus_tag="PK754_11375"
     CDS             complement(20..574)
                     /locus_tag="PK754_11375"
                     /inference="COORDINATES: protein motif:HMM:NF009807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HNB10149.1"
                     /translation="MTHDERGILIKKIKDLPKNLEKAIAGLNKKQMDTPYREGGWTPR
                     QVVHHIADSHMNAYIRAKLAVSEDHPTIKPYDQDAWAEMEDGKSLPVDYSMTIIKALH
                     ARWGQFWDRVQESDWSRTVMHPANGVMSLERILLTYANHGEKHCEHINGLRVRMGWTG
                     KNKPTKKAKAKQVKKTSKNKRKRG"
     gene            complement(604..1002)
                     /locus_tag="PK754_11380"
     CDS             complement(604..1002)
                     /locus_tag="PK754_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006835736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNB10150.1"
                     /translation="MMRYVVLWVFMASVLCAQEEKKPADKNTGKKQFVYVLKLRPDLL
                     KEENWKEADNAAVGRHFNYLKNLLAEGTLIFAGRTDVMNENTFGIAVFWATDESEAQK
                     IMAGDAAVQAGVMTAQLLPFKVALMQNNNK"
     gene            complement(999..1523)
                     /locus_tag="PK754_11385"
     CDS             complement(999..1523)
                     /locus_tag="PK754_11385"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB10151.1"
                     /translation="MKTKDVSVVTTYLAMHQKPSIETVAMPSDGVILRAENPTVKFYR
                     FLYDAVGADWNWIDRKEMADEELAKETQDALVEIYVLYDRGVPAGYAEIDRRIAGEIE
                     LKYFGIIPEFISKRYGRFLLTWIIEKAWQYKPQRFWVHTCSLDHPRALPLYQNLGFEI
                     YKTETHTVTIPEVL"
     gene            complement(1520..1975)
                     /locus_tag="PK754_11390"
     CDS             complement(1520..1975)
                     /locus_tag="PK754_11390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB10152.1"
                     /translation="MIWKNETYVIDTDPKNLDVDVIHDFLGASYWAKDIPRSVVEKSI
                     RHSLCFGIYHETKQIGFARVITDYATFAYLADVFVVASHRGKGLSKWLMGCIQSHPEL
                     KNLRRWMLATRDAHGLYKQFGFIVTAKPDRLMEILDMDVYTKIAKENRP"
     gene            complement(2004..2630)
                     /locus_tag="PK754_11395"
     CDS             complement(2004..2630)
                     /locus_tag="PK754_11395"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HNB10153.1"
                     /translation="MFIPKRYRITDIDTIRAFVAEYEFGTLFSQSAGKPWATHIPMQW
                     FSDGSPHGVLRTHISKANSQWKDMEGQTVLAVFLGPHVHITPTWYDHVNVPTWNYASV
                     HIMGCARIIEDPKAIREMLTVLVDTYESAHGNTYSVQSLPEDYYNREVQGLVGIEIQI
                     ENVDAAFKLSQNRHRSDYENIICQLRNSNDENSHRIADLMEKHTPEFS"
     gene            complement(2634..3527)
                     /locus_tag="PK754_11400"
     CDS             complement(2634..3527)
                     /locus_tag="PK754_11400"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNB10154.1"
                     /translation="MSYVIWGFVPIFLKKLQGFDDYEIIFYRILLAALAMTGVAILLW
                     GETSQAIGGLYRRSKKDFLHMLILTSIGGGLLATNWVTYVYVVNHVSINAASFSYLIL
                     PIATAFLALILLKEKLNAMRWAAVAMSGVSCYLMANVDARQMAYIAAITLSYSFYLIT
                     QRKNTYMPRRTNLALQMIVGTALLLTMDPIHTAPIDLPMHFWINVTIIAVVFTVTPLL
                     LNLYALNGMGSSQLAFLIYINPIISFVTAIVWYGEDLNELAVISYSLLAVAILLFNWE
                     IIVKAAEKTGLRAATKTITAE"
     gene            3963..4283
                     /locus_tag="PK754_11405"
     CDS             3963..4283
                     /locus_tag="PK754_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10155.1"
                     /translation="MVFEFEAGAGHTKSNGSFSDMKIYSLRYDGRIKSFSPFHALSYL
                     DFSYRHKNDDGFRTDIKQAIIGASIHLVETKVLIVASDISFSKSFADYGTAKQIALAS
                     VRLF"
     gene            4328..4522
                     /locus_tag="PK754_11410"
     CDS             4328..4522
                     /locus_tag="PK754_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10156.1"
                     /translation="MTSKVDSYDSVGVGVINGLKIVFTSWEDVFGVALEKTPKKNRSS
                     RLNKWRKTWNRLQNFPSLTS"
     gene            complement(4858..5367)
                     /locus_tag="PK754_11415"
     CDS             complement(4858..5367)
                     /locus_tag="PK754_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10157.1"
                     /translation="MKKIIFFLLLTYAASAFSQDLGWQRVRLICGSAWSFTSGDEYLY
                     TAIQIPVTKKIFVGLCGGYRPQDVKTVHADFTVGYDLVTSGPNRLFFKTDGSFFVREG
                     SFIFDARFFRQTIGLGYTSFILQKIVLTTEVAILGFYYGSRKDPPPSQKHPSVFGAGE
                     LKIGIGYKF"
     gene            complement(5364..5894)
                     /locus_tag="PK754_11420"
     CDS             complement(5364..5894)
                     /locus_tag="PK754_11420"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNB10158.1"
                     /translation="MKKLILVLLFSSISLQAVAQENNRERPNIIIGGDVSPLSRGSGT
                     EDHFFIAGNYPINQKIHVGLSYGFLAKDAVSIQHEKLHQIGSALGYRLGQSGSFLFFA
                     KTELAFFYKPDVTKRLDKSFFRHTVGGGVWTRLMNRFFLNTEINIGGFFYGRNEPVKY
                     QFYNAADIKTGIGFQL"
BASE COUNT         1681 a         1373 c         1229 g         1767 t
ORIGIN      
        1 tcttcgcagc gtatgatatt catccgcgtt tccttttatt cttcgatgtt ttttttacct
       61 gttttgcttt tgcttttttg gttggtttgt tttttcctgt ccagcccata cgcacacgca
      121 aaccattgat gtgctcgcag tgtttttcac cgtgattggc atacgtcaaa agaatacgct
      181 ccagtgacat tacaccgttt gcgggatgca tgactgtccg cgaccaatct gattcttgca
      241 cgcgatccca aaactgtccc catcgtgcgt gcaacgcttt gatgatcgtc atcgaataat
      301 caaccggtaa actcttgccg tcttccattt ccgcccaggc atcctgatcg tacggtttga
      361 tcgtcggatg atcttccgac acagccagtt ttgcccggat ataggcattc atatgggaat
      421 cggcgatatg atgcacaacc tgacgcggcg tccatccgcc ttcacgatac ggcgtatcca
      481 tttgtttctt gttcaagccg gctatggctt tttctaagtt tttgggtaag tctttgatct
      541 tttttatcag tatcccacgt tcatcgtgtg tcatatcggt tccttttatt tttacagccg
      601 acattatttg ttattgtttt gcatcaacgc cactttgaat ggcagtagtt gggctgtcat
      661 tacgcccgct tgcacagcgg cgtcaccggc catgattttt tgcgcctcac tttcatccgt
      721 cgcccaaaac accgcgatac caaacgtatt ttcattcatc acatccgttc gccctgcaaa
      781 aatcaacgta ccttcagcca ataagttttt gaggtaatta aaatggcgtc cgacggctgc
      841 gttatctgcc tctttccagt tttcttcttt gagtaaatcc ggccgaagtt tgaggacata
      901 tacaaattgt tttttaccgg tatttttatc ggccggcttt ttttcttcct gcgcacaaag
      961 tacgctcgcc ataaaaaccc atagaaccac ataacgcatc atagaacctc cgggatggta
     1021 acggtgtgtg tttcagtttt gtatatttca aatccaaggt tttgatacag cggtaaagct
     1081 cttgggtgat caagcgaaca cgtatgcacc caaaaacgct gcggcttata ttgccacgcc
     1141 ttttcgataa tccaggtcag caaaaagcgg ccgtaacgtt tactgataaa ttccggtatg
     1201 atgccaaaat atttcaattc gatctccccg gcaatgcgac gatctatctc cgcataacct
     1261 gccggcacac cgcgatcata cagaacataa atttccacca gtgcatcctg cgtctctttg
     1321 gccaattctt catccgccat ttcttttcga tctatccaat tccaatccgc acccaccgca
     1381 tcgtacagaa accgataaaa ctttaccgtc ggattttcgg cgcgaaggat gacaccatcg
     1441 gacggcatcg ccacggtttc gatggacggt ttttgatgca tcgcgaggta cgtcgtgacg
     1501 acgcttacgt ctttcgtttt catggacggt tttccttggc gattttcgta taaacatcca
     1561 tatctaggat ttccatcaac cggtccggtt tcgcagttac aataaatcca aattgtttgt
     1621 aaagcccgtg tgcatcgcgt gtggccagca tccatcgtct cagatttttc aattcgggat
     1681 gactttgaat acatcccatc agccatttgg acaacccttt accgcgatgc gaagcgacaa
     1741 caaaaacatc ggcaaggtat gcaaatgtag cataatccgt gatcacccgt gcaaaaccta
     1801 tttgttttgt ttcatggtag atgccaaaac aaagtgaatg acgtatggat ttttccacga
     1861 cggatctggg aatatccttt gcccaatacg acgcacctaa aaaatcatga atcacatcca
     1921 catccagatt tttcggatcg gtatctatca cgtacgtttc gtttttccaa atcattgttg
     1981 cgcccttagt aaacatttaa aatttaagaa aattccggcg tgtgtttttc catcaggtcg
     2041 gctatccggt gggaattttc atcgttgctg ttacgtaatt ggcaaataat attttcataa
     2101 tccgaccggt gacgattctg actcaatttg aaagcggcat ccacgttttc gatttgtatt
     2161 tctatcccca ccaaaccttg cacttcgcgg ttgtaataat cttccggtaa ggattgaaca
     2221 gaatatgtat tgccgtgtgc cgattcatat gtatctacca atacggtgag catttcacga
     2281 atggctttcg gatcttctat gatacgggca caccccatga tgtgtacgga cgcatagttc
     2341 cacgtcggca cattgacatg atcataccat gtcggcgtga tatgcacatg cggccccaga
     2401 aaaaccgcca aaacggtctg gccttccatg tctttccatt gcgaattggc tttggatata
     2461 tgcgtacgga gaacaccgtg cgggctcccg tcggaaaacc actgcatcgg tatgtgtgtg
     2521 gcccacggct tgccggccga ttgactgaat aacgttccaa attcgtactc cgccacaaac
     2581 gcacgtatcg tatctatatc cgtgatccgg tatcgttttg gaatgaacat acgttactcc
     2641 gcagtgatgg tttttgttgc ggccctcaat ccggtttttt cggcggcctt gacgatgatc
     2701 tcccaattga aaagcaaaat ggccaccgcg aggagactgt atgagatcac agccagttcg
     2761 ttcaaatctt ctccgtacca aacaatcgcc gtgacgaaac tgataatcgg gttgatgtaa
     2821 atcaaaaacg caagctggga cgaacccata ccgttgagcg cgtaaagatt gagtaaaagc
     2881 ggtgtcacgg taaaaacaac cgcaatgatc gtgacattga tccagaagtg catcggcaaa
     2941 tcaatcggtg cggtatgtat gggatccatc gtcaaaagta atgctgtgcc aacgatcatt
     3001 tgcagagcaa gattggtacg gcgcggcata tacgtgtttt tacgttgagt gatcagataa
     3061 aaactatacg acagcgtaat cgccgctatg tatgccatct ggcgtgcgtc cacattggcc
     3121 atcaaataac aactgacacc gctcatcgct accgcggccc accgcatcgc attcagtttc
     3181 tctttgagca agatcaatgc caaaaacgca gtcgctatgg gtaaaatcaa ataggaaaac
     3241 gaggcggcgt tgatactgac gtgattgact acataaacgt acgttaccca attcgtcgcc
     3301 aaaagtcctc cgccgataga tgtcaatatc agcatatgca aaaaatcttt ttttgagcgg
     3361 cggtacaatc ctccgatcgc ttgcgaggtc tcaccccaca aaaggatcgc cacacctgtc
     3421 atggccaaag ccgccagcaa aatgcgataa aaaattattt cgtaatcatc aaatccttgc
     3481 agttttttca aaaaaatggg aacaaacccc caaatcacat aagacattaa accgctaaaa
     3541 aagtactttt tggagctcat aatgaaatat agtccaattt tttaattaat aaatagtcca
     3601 atgtatcatt cgtttgataa aggtagtcca atataggata catttgttcc atcaaaaagt
     3661 accaattttt atgttttcag tatagaccaa tgtgtttttt tgaagaaaat gatcaccctg
     3721 tttattcgat tgtgatcaca aacggaattc ggaataatgt tctgaatgac tatatccatc
     3781 ttgtgtaggt aaaagtaata aacgtatagg gtttttaagc tacctcgcct tttcatactt
     3841 tatttttgcc ggaaaagata ttttgagaaa aatgagggtg taacagttgg ctcaggcggc
     3901 gatttctgag gggtgaaggt ggaattgtca caaaatcatc gtatggactt ttagttttgg
     3961 tcatggtttt tgaatttgaa gcgggggctg gtcatactaa atccaacgga agtttctcgg
     4021 acatgaaaat ctattcgctg cgatacgatg ggcgtatcaa gtcgttttct ccttttcatg
     4081 cacttagtta tttagatttt tcctaccggc acaagaatga cgacggtttt agaaccgata
     4141 ttaagcaggc aattatcgga gcctcaatac atttggtaga aacgaaagta ttaatagtag
     4201 catctgatat ttcgttttca aaatcttttg cagattatgg aactgcgaaa caaatagctt
     4261 tggcgtcggt aagactattt tagcacgtga caataataat gtcggtgggc ttcttaaggc
     4321 cggagtagtg acgagcaaag tggacagcta cgactctgtc ggtgtcggag ttattaacgg
     4381 tctcaaaata gtatttacat catgggaaga tgtatttggg gtggccttgg aaaaaacgcc
     4441 gaaaaagaac aggagttcgc gacttaacaa atggcggaaa acgtggaaca gacttcaaaa
     4501 cttcccatcg ctgacgtcat aggaactgtt ttccgccgcc acgtaagcga tctccggttc
     4561 cggcgttttt tccacagcct tgatttttct tttggttctt ttctttgcat caaggcaaag
     4621 aaaagaacat ggttaattta aatactattt caagacaatt aatgtctttg gataattgcg
     4681 atccgaaagc ggcgtaagta attagtcaga gcatgtatcg tggaagcgat tggtttcttc
     4741 tctgttgcgt tttcatcgga tattcgaatc cacacacatc aagaccacct gtaaaacaga
     4801 aataaccgca aaaaccaatc gcatacccgc cttcaaccgt atcggcgttt aacgatgtta
     4861 aaacttataa cctatgccaa tttttaactc accggcacca aaaacggagg ggtgtttttg
     4921 acttggcggt ggatctttcc ggctaccata gtaaaacccc aaaatggcta cttctgtcgt
     4981 taagactatt ttttgaagta taaacgatgt gtagcctaat cctattgttt gtcggaaaaa
     5041 acgcgcgtca aaaataaaac taccttcccg tacgaaaaaa gaaccgtctg ttttaaaaaa
     5101 taaccggttg ggtcctgatg taaccaagtc gtaacccaca gtaaaatccg catggacggt
     5161 ttttacatct tgaggccgat atcctccaca aagcccgaca aatatttttt ttgtaacagg
     5221 aatttgaata gccgtatata aatattcgtc accggatgta aatgaccacg cagagccgca
     5281 gatcaaacga actctctgcc atcccaaatc ctgcgaaaat gctgatgcgg cataggttag
     5341 tagaagaaaa aatataattt ttttcataac tgaaacccta tacctgtttt tatgtcagcg
     5401 gcattataaa attgatattt gacgggttca ttgcgaccat agaaaaaacc accaatattt
     5461 atttctgtat tcagaaaaaa acggttcatc agtcgagtcc atactcctcc acccacggta
     5521 tgtctgaaga aacttttatc taaccgtttt gtgacgtcgg gtttataaaa aaaagcgagc
     5581 tctgtctttg caaaaaaaag aaaacttccc gattgtccaa gacgataccc taaagcgctg
     5641 ccgatttgat ggagtttttc gtgctggatc gaaaccgcat ctttagcgag aaaaccataa
     5701 ctcaggccga catgaatttt ttggttaata ggataattac cggctatgaa aaaatgatct
     5761 tccgtgccgg aaccacggct taagggtgaa acgtcccctc cgattataat attaggacgt
     5821 tcccggttat tttcttgagc aaccgcttgc aatgaaatac tactgaaaag taagacaagg
     5881 ataagttttt tcataaaatc accaatctgc tttataagtt actttttttc gatcggcgga
     5941 ataattacca aatacgccga caacattttt tcctttgata ttagagcgtt tttcaatggg
     6001 aatatcgtaa agcgtaatat cccaatcacc aaattcgggt gtcatactat
//
LOCUS       DAOQNQ010000198         6026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_5149_length_6026_cov_5.730698, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000198 DAOQNQ010000000
VERSION     DAOQNQ010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5149_length_6026_cov_5.730698"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PK754_11425"
     CDS             <1..721
                     /locus_tag="PK754_11425"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNB10159.1"
                     /translation="AASYMNHILDIGSGFASYVQPLPRWGGNIGVGITYFTYGEFEGY
                     DANGAATSSFHANDMAWHVFYARMLDEHISVGGTFKYIRSTIDEFTSSAWAMDAGVQY
                     SPEERVRLGIVLLNAGFVSDPFIRHKESLPLSLQAGVNYKLRNAPVTVNLTFTDIQND
                     LVKSPVVGAEWMLHESWSVRLGYSLQRDRELHAGSSDFLDRMAGASAGIGFKYDKYIV
                     DYSFASWGIGTLNRFSIQYQF"
     gene            737..1294
                     /locus_tag="PK754_11430"
     CDS             737..1294
                     /locus_tag="PK754_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10160.1"
                     /translation="MTLFENHSFMLLIFNALVVFTVLANIYQELYAVIRFSERTMGKA
                     QFYQATAGRLETIVYWSGFFVTLLISALILLSDAENHTNLVACIALAVGFFVNAVVSR
                     ICFFNEIGIGSVNVQSEMEFQWNEIKSFYWQNNVLHLQLRRKLFGHIRMRFSDSTSIL
                     LINERLHRLADSGVSLRTESAPIQG"
     gene            1322..3439
                     /locus_tag="PK754_11435"
     CDS             1322..3439
                     /locus_tag="PK754_11435"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNB10161.1"
                     /translation="MKLEVKRHNKKLWLSMIVVVAVLVGLNVAHAPAFRSWEESGLDF
                     LFKIRTDVPEQIPVVVVMVDEKAYEKDFGYYDPLPRRYLARLIDTLKAKGAKTIALDI
                     AFYDKLDQLDARGDTLLRDAMHRARNVISVSIWHQREDGSIRIQEPDPFFRPALQAVG
                     YANLQISGGGGLASVREVRPVATMSDNTMLLSFSAAAYCQMMDLPAEEFIRDIRENRW
                     QLLPRIPRDAQGNMLINYISAPSEWVKQEDGTWTQMTPSRITTYRSSQLTGDIEWPED
                     IFKDKLIVVGNGSEFVPDRFITPFFEQTRRNWMYGAEVHANAFLTLLNKSFIDKPGWP
                     VIALCMLLLSITMVMATVRLGFAAEMATLTVLLITVWTGAHILFAGGWWIPAVSLSLV
                     VLLSYFMTSIFQAFTEEHDKKQIKGMFSRYAPPAYVDQLVLDPSKLELGGEEKEISIL
                     FSDIEGFTSISETMAPKQLVELLNDYLNAMTQRIFQQGGTLDKYIGDAIVAVFGAPLP
                     QNEHALHACYAALDMQSSLEEFRAKWMAKGMPAIRNRIGVNTGRVVFGNIGSDIRYDY
                     TGIGDAMNLASRLEAANKQYGTYLMISEFTHAQVRERVIVRELDMIVVKGKTKPVKVY
                     ELMGRANEPLPENTRHLLARYQEGIDLYHKREWSEAIRRFEQALTYAHTDEPSKLYIH
                     RCKQFIAEPPAPDWDGTYHLTSK"
     gene            3565..4515
                     /locus_tag="PK754_11440"
     CDS             3565..4515
                     /locus_tag="PK754_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10162.1"
                     /translation="MKSFMLTWFVALALGLSAVNGQDNGVAFLSSIKGTIKVTDASGK
                     EKKASPSLKLLAGDKITASANSSVSVMYYSGKEVVLKAGQNHVITAVKTEDSFLSGLY
                     KSLSGMLWGEASNSSMAGATRAWAGDANRNITAVYPSESKVINDSPVFEWADHRASPG
                     NDYVITISSEISDFKYEMTIKGSKQAAYPKTAPKLKSDEKYQWSIKDATGAEVSPTAK
                     FSLLAPEDKETLEDDIKEVAKVCNNDNTNPQWYLLSAALYRDYGLMKEAETAIKALIA
                     MKPDMVQAHVMLATLYKETGRLEEAKAAEEQARKLSEGKK"
     gene            complement(4630..5796)
                     /gene="lpxB"
                     /locus_tag="PK754_11445"
     CDS             complement(4630..5796)
                     /gene="lpxB"
                     /locus_tag="PK754_11445"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNB10163.1"
                     /translation="MHNLTPQHIILLAGEKSGDQYGALLAQRLLSINPAIRLSGIGGD
                     TMQKAGVQLWENTTHHSVMGFVEVFRSIRYWLKLRKKIIRLILTEKPDAIILIDFPDF
                     NLSVARSIKKTLGERSPFIFYFIPPQVWIWRKKRIHFIAKLCHTVYPLFSFENDLYQS
                     RGIRSRYFGHPIRDMLSAGKYPQNPHQKQDNHITLGLLPGSRNQEVVRILPIMLQSVL
                     LYSQRSRQPLHLYISRVGEVDAFIYETIHRRFPTLNITWCHTGDEAITHSDAILAKSG
                     TVNLELIYHRKASLIVYRTGTLTYLIARVWLRIRHISLINILAGNTVVREFIQHHARP
                     ESIAQEIDRLLHDDVYRHDMLGELDKLHTALFPESESPSAVNQIAADMLSITSR"
     gene            5844..>6026
                     /locus_tag="PK754_11450"
     CDS             5844..>6026
                     /locus_tag="PK754_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10164.1"
                     /translation="MDEQLRAALGDLIAIEEIVFEWDTAYVALRTPQINTDIMAQIEA
                     RLAPLGLKSSIASINRV"
BASE COUNT         1560 a         1292 c         1561 g         1613 t
ORIGIN      
        1 tgcagccagc tatatgaatc acatccttga tatcggttcg ggttttgcaa gttatgtcca
       61 gccgttgccg cgttggggtg gtaatatagg tgtaggaata acgtatttca catacggcga
      121 atttgaaggc tacgacgcca atggtgcagc gacgtcatcg tttcacgcca acgatatggc
      181 gtggcatgtt ttttatgcgc gcatgttgga tgaacacatt tccgtcggcg ggacattcaa
      241 atatattcgg agcacgattg atgaattcac atcttccgca tgggcgatgg atgccggtgt
      301 gcagtattca cctgaggaac gtgtgcgtct gggcattgtt ttgctcaatg cgggttttgt
      361 cagtgatccg tttatccgtc ataaagaaag cctgccgtta tcattgcaag ccggcgtaaa
      421 ttataaactg cggaatgccc cggttacggt caatctgaca tttacagaca tacaaaatga
      481 tctggtcaaa agccctgtag tcggcgcaga gtggatgctg catgaaagtt ggtcggtacg
      541 tctcggttac agcttacaac gtgaccgtga attgcatgca ggttcttcgg attttttgga
      601 tcgtatggcc ggagcctctg ccggtatagg atttaaatac gataaatata tcgtggatta
      661 ttcatttgca tcatggggca tcggtacact caatcgtttt tcaattcaat atcaattctg
      721 aaacatacgg cacggtatga ccttgttcga aaatcattcg tttatgttgc ttatcttcaa
      781 tgcgcttgtg gtatttacgg tattggccaa tatctatcag gaattatatg cggtcatccg
      841 gtttagcgaa cgcacgatgg gcaaagcaca gttttatcag gcgaccgcag ggcggttgga
      901 aactatagtg tactggagcg ggttttttgt cacgctgctt atcagtgcgc tgattctctt
      961 atccgatgct gaaaatcaca ccaatctggt cgcatgcata gcattagccg tcggcttttt
     1021 tgtcaatgcg gttgtgtcac gcatttgctt tttcaatgaa atcggtattg gcagtgttaa
     1081 tgtccagtcg gaaatggagt ttcaatggaa tgagataaaa tcgttttatt ggcaaaacaa
     1141 cgtactgcat cttcagttgc gtcgaaaatt attcggccat attcgtatgc ggtttagcga
     1201 tagcacatct attctattaa tcaatgaacg cctgcaccgc ttggcggact cgggcgtatc
     1261 tctgcgcacg gaaagtgcgc cgatccaagg ataaattgtt ccccattttc cggagttgtg
     1321 tatgaagtta gaagtcaagc gccataataa aaaattgtgg ctcagcatga tcgtcgtcgt
     1381 cgccgtatta gtcggactca atgtggcgca tgcgccggca tttcgcagct gggaagaaag
     1441 cggtttggat tttttgttta agatacgaac cgatgtgccc gaacaaattc ccgtcgtggt
     1501 tgtgatggtg gacgagaagg cgtacgaaaa ggactttggg tattatgacc cattgccgcg
     1561 gcgttatctt gcgcgattga ttgatacact caaggccaag ggcgcaaaaa caatcgctct
     1621 cgatattgct ttttatgata agttagatca actcgacgcc cggggcgaca cacttttgcg
     1681 cgatgcgatg caccgtgccc gcaatgtcat ttcggtttcg atttggcatc aacgtgaaga
     1741 cggttccata cgcatacagg aaccggatcc gtttttccgt cccgctttgc aagccgtggg
     1801 atatgccaat cttcagattt ccggcggcgg cggtttagcg agtgtgcgcg aagtgcgtcc
     1861 ggtggctacc atgtccgaca ataccatgtt gctttctttt tcagcggcgg cttactgcca
     1921 aatgatggat cttccggcgg aggaatttat ccgtgatata cgtgaaaatc gctggcagct
     1981 tttgccgcgt attccgcgtg atgcccaagg caacatgctg atcaactata tcagcgcgcc
     2041 gagcgaatgg gtgaaacagg aagacggtac gtggacgcaa atgacgccga gccgcattac
     2101 gacctaccgc agtagtcagc tgaccggcga tatcgaatgg ccggaagata ttttcaaaga
     2161 taaattgatc gttgtcggaa acggttcgga atttgtaccc gatcggttta tcaccccgtt
     2221 tttcgaacaa acgcgccgca attggatgta cggcgccgaa gtgcatgcca atgcatttct
     2281 caccctgctt aataagtcat ttattgataa acccggctgg ccggtgatcg cactgtgtat
     2341 gctgttgctc agtatcacta tggtgatggc tacggtacgt ttgggatttg ccgccgaaat
     2401 ggccacgctg accgttcttt taatcaccgt atggaccgga gcgcatattc tgtttgccgg
     2461 gggatggtgg attcccgccg taagcctgtc tctggtggtt ctgctttcat attttatgac
     2521 atccatattt caggcgttta cggaagaaca cgataaaaag cagatcaaag gaatgttttc
     2581 gcgctacgcg ccaccggcgt atgtagacca acttgtgttg gatccgtcga aactggagct
     2641 tggcggcgaa gaaaaagaaa tcagtatttt gttttcggat atcgaaggat tcacgtctat
     2701 ttccgagacg atggcgccga aacaattggt cgaattgctc aacgattatc ttaacgcgat
     2761 gacgcaacgt atttttcaac agggcggtac gttggataaa tatatcggcg atgcgattgt
     2821 ggccgtattt ggtgcgccat tgccgcaaaa tgaacatgcg ctgcatgcgt gttatgctgc
     2881 gctggacatg caatcttcct tggaagaatt tcgcgctaaa tggatggcca aaggtatgcc
     2941 ggccattcgc aatcgtatcg gtgtgaatac cggccgggta gtttttggta atatcggttc
     3001 ggatattcgg tacgattata caggcatcgg tgatgccatg aacctggcat cgcgcctcga
     3061 agcggcgaat aaacaatacg gcacgtatct gatgatcagc gaatttaccc atgctcaggt
     3121 acgtgagcgg gtgatcgtgc gcgagctgga tatgatcgtc gtcaaaggca aaaccaaacc
     3181 ggtcaaagtg tacgaactta tgggtcgtgc taatgagcct ttgccggaaa acacacgcca
     3241 tcttttagca cgttatcagg aaggtattga tctgtaccac aaacgtgagt ggagcgaagc
     3301 gatccgccga tttgagcaag cgcttacgta tgcgcatacc gatgaaccgt ccaaactgta
     3361 tattcaccgc tgtaagcagt ttatcgcaga accgccggcg ccggattggg atgggacata
     3421 ccatctgaca tcgaaataag ccttgagttt acaagaggtt ttaaattata ttcgcgcgaa
     3481 gcggtttacc tgatgacttt cgtttgtatt cacatcagtt caacaaagtt ttgagatgag
     3541 tattctaaca tagagaggtg aggtatgaag tcatttatgt taacatggtt cgtcgcattg
     3601 gctttgggcc tttcggcggt taacgggcag gataacggtg tcgcgttcct gtcatccatc
     3661 aaaggcacga tcaaagtaac cgatgcgtcg ggtaaagaaa aaaaagcatc gcccagtctc
     3721 aaattgctgg ccggcgataa gatcacagcc agcgccaaca gttccgtcag tgtcatgtat
     3781 tacagcggca aagaagtcgt tctgaaagcc ggacaaaatc acgtgattac ggccgtcaaa
     3841 acggaagatt cattcctttc gggattgtac aaatctctgt cggggatgtt gtggggagaa
     3901 gcttctaatt cgtccatggc cggagcgaca cgcgcctggg ccggtgatgc caatcgtaac
     3961 attactgcgg tatatccttc agaatccaaa gtcatcaacg atagtcccgt tttcgaatgg
     4021 gccgatcacc gcgcctcacc gggcaacgac tatgtgatca cgatttcgag tgaaatttct
     4081 gattttaaat acgaaatgac catcaaaggt tcgaaacaag ccgcgtatcc taaaacagcg
     4141 cccaaattga agtctgatga aaaatatcaa tggtccatca aagatgcgac cggcgctgaa
     4201 gtttctccga cagccaagtt ttcattactg gctccggaag ataaagaaac gttggaagat
     4261 gacatcaaag aagtggccaa agtttgtaat aatgataata ccaatccgca gtggtatctt
     4321 ttgagtgcgg cattgtatcg cgattacggt ttgatgaaag aagccgaaac ggccatcaaa
     4381 gcgttgatcg ccatgaagcc tgatatggtg caagcgcacg tcatgctcgc tacactgtac
     4441 aaagaaacag gccgactgga agaagccaaa gccgccgaag agcaagcacg caaattgagc
     4501 gaaggcaaaa aataaaattt aatacggttc agcttttaag aggctgtttc aaaagttcaa
     4561 cggataagat ttttttgtcg catgaaaaaa tcaagttgag cggctgaaac agcctctttt
     4621 atttttctgt tatcgagaag ttatactgag catgtctgcg gcgatctgat ttacagccga
     4681 tggggattcc gattcgggaa ataatgcagt gtgcagtttg tccagttcgc cgagcatgtc
     4741 atggcgatac acgtcatcgt gaagtaggcg gtcgatttct tgtgcgatgg attcaggccg
     4801 ggcgtggtgt tgtataaatt cacgcacgac ggtatttcca gcgaggatat tgatcagcga
     4861 aatatgacga atacgcaacc agactcgagc gatcaggtag gtgagtgtgc cggtacgata
     4921 cacgatcaac gacgctttac gatggtagat cagttcgaga ttgacggttc ccgatttggc
     4981 caagatggcg tcactgtgtg ttatcgcttc atcgccggta tggcaccatg taatatttaa
     5041 tgtcggaaaa cgccgatgga tcgtttcata gatgaacgca tctacttcgc cgacgcgact
     5101 tatatacaga tgaaggggtt gccttgatcg ttgcgaataa agaaggacgc tttgcaacat
     5161 gatcggaagt atgcgaacaa cttcttggtt gcgactgccc ggaagaagtc cgagcgttat
     5221 gtggttatct tgcttttgat gtgggttttg gggatatttt ccggcgctaa gcatgtcgcg
     5281 aatcggatga ccgaagtagc gacttcgtat tccgcggctt tgatataagt cgttttcaaa
     5341 cgaaaaaagc gggtacacgg tatggcataa tttggcaatg aagtgaatgc gttttttacg
     5401 ccaaatccaa acttgcggtg gaataaaata aaaaataaaa ggactgcgtt ctcccagcgt
     5461 tttttttatc gagcgtgcga cggatagatt gaaatccgga aaatcaatca agataatagc
     5521 gtcaggtttt tcggtgagga taagtcggat tatttttttt cgcagtttta accagtacct
     5581 gatcgaacga aagacttcaa cgaagcccat gacagaatga tgcgtggtat tttcccatag
     5641 ttggacaccg gctttttgca tcgtatcacc gccgataccg gacagtcgga tagccggatt
     5701 gatactcaga agtcgctgtg ctaataaagc gccatattga tcgcccgatt tttcaccggc
     5761 gagtaagatg atgtgctgtg gagtgaggtt atgcatcggt gcgatgataa tgtaaataag
     5821 atttaaaatc acggataatg ataatggatg aacaattgcg cgccgcgctg ggcgatctga
     5881 tcgctatcga agaaatagtt ttcgagtggg atactgccta cgtagctttg cgcacaccgc
     5941 aaatcaatac ggatattatg gcgcaaatcg aggcgcgttt agcgccgctc gggcttaaaa
     6001 gttcgattgc cagcattaac cgtgtg
//
LOCUS       DAOQNQ010000199         5961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_5238_length_5961_cov_6.003386, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000199 DAOQNQ010000000
VERSION     DAOQNQ010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5238_length_5961_cov_6.003386"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1568
                     /locus_tag="PK754_11455"
     CDS             <1..1568
                     /locus_tag="PK754_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III deoxyribonuclease"
                     /protein_id="HNB10165.1"
                     /translation="QKTSTVAIVQKAFRFFEAKGISANEIVKRIQANFKPENCLSANN
                     DAEAEQNQILLNTLEDENNPIRAVFAVQKLNEGWDVLNLFDIVRLYEDRDGKDGKPGI
                     TTLSEAQLIGRGARYFPFAIEEGHDVFTRKFDDDISNDLKTLEELYYHTKEDSRYISE
                     LKKALVDSGIYEDEENLVTKQLRLKIDFKETEFYRDGHVFFNKKILKRSDNIKSFADL
                     GVKRTNYRHILSSGVGRMSSAFFEMEPTQSGEEIIKSKDVKLTDIPKNTIRFALSQNP
                     FFYFDSLSYYFPSVGSLSNFIDSKELLASLEITFNGTANRLKEINHFDYLQALNGLLQ
                     SIEADIKSNSTEYEASDYIKEPIHKVFKDKEIKVYKDSVRADGQETLVANEPWYVYNA
                     NYGTSEEKKFVELFSRRFEGLNLKFENIYLIRNEREIKIFDKLGRAFEPDFLLFCKQR
                     DGEQMTFQVFIEPKGNHLLSYDKWKEDFLKEIRNEQKAIKIHTDTYLITAVPFYNYDN
                     ENEFRITLENTLK"
     gene            complement(1579..2478)
                     /locus_tag="PK754_11460"
     CDS             complement(1579..2478)
                     /locus_tag="PK754_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNB10166.1"
                     /translation="MPCTPVTVRTILNKTKRRDPWFLDDYTLNPYSGCSMGCVFCYVH
                     GSRYGTHTEKKLSYKTNAPELLDKQLSLRAKRGQYGFIVLASSTDPYVHAEKELGLTR
                     RLLEIILHHRFPVHIITRSDLILRDTDILMHIGKTAILPEDLLGKVPSGALITFSFSS
                     VDDRTAHIFEPGATPPSRRLQGLKQILDEGFYAGVSLMPLLPWISDTADQLELSFSTF
                     AQIGARYIFPASLTLYGDQPSDSKIRTLRTIQQHYPHLMDKYRGYFDRSATMPDYYLT
                     ALEEKLNGLCEKYRLPNKVHLND"
     gene            complement(2607..2747)
                     /locus_tag="PK754_11465"
     CDS             complement(2607..2747)
                     /locus_tag="PK754_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10167.1"
                     /translation="MDGHKIPFLDASMLVWFKEQSLRERDQMDVSALKHLLKNKTGTD
                     EN"
     gene            complement(2918..3082)
                     /locus_tag="PK754_11470"
     CDS             complement(2918..3082)
                     /locus_tag="PK754_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10168.1"
                     /translation="MGVGEHIRKQIGQSKKRPTELVAELNEFLKKIGKKPKQENNERI
                     RKIVGPADKK"
     gene            complement(3085..4014)
                     /locus_tag="PK754_11475"
     CDS             complement(3085..4014)
                     /locus_tag="PK754_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNB10169.1"
                     /translation="MNFTPKKNTGLGKGLSALIREELNADGTLKDEVRSDTPIAKIPI
                     DQIRANPFQPRKEFEPQALEELKQSILQNGIIQPITVRKTATGYELIAGERRFRASKL
                     AGLTELPAYIRENVTDQEMLELAIIENVQREKLNPIELAEGYQLLIETCNLTQDQVAQ
                     KIGKDRTTITNIIRLLKLPADIQESLKKEEITIGHGRALIALPSEQLQLKAWKQIVDG
                     GMSVRKTEQLVKTTLEELEGKSHKVSPNKYEIESAQPANPHIADVEGRLRMKLGTKVR
                     LHHHKDGSGSVEIEYYSNDDLQRVIEMIETIRG"
     gene            complement(4027..4806)
                     /locus_tag="PK754_11480"
     CDS             complement(4027..4806)
                     /locus_tag="PK754_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004893255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB10170.1"
                     /translation="MGRIIAIANQKGGVGKTTTSINLSASLAHAGKRTLIIDMDPQAN
                     STTGLGFDLEKIERGSYEVLIQTVPATDTILKTMMPSMDLLPAHIRLVGAEIELVNLE
                     EREHMFKKAIAPIRDQYEYILIDCPPSLGLLTLNALSAADTVLIPIQCEYYALEGLRQ
                     LLNTIRLVQRHLNTDLTIEGVLMTMYDSRLNLSNQVVGEVKEYFKDKVFKNVIHRNIK
                     LGEAPSFGKPIILYDPASVGSQDYMGVAKEVIANVITPQPA"
     gene            complement(4934..5776)
                     /locus_tag="PK754_11485"
     CDS             complement(4934..5776)
                     /locus_tag="PK754_11485"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing transcriptional regulator"
                     /protein_id="HNB10171.1"
                     /translation="MINSIDDLQRQIEIAALIYEFPDKYSEIDLAEKFHTSESSIHRD
                     MRALRAMGITVNSRKRAFRIELSLLQINTLITTYFAFGNNETIKNLPLVLERFQNKTL
                     SFFVETIRAIRQKHVIEIEYRSAKTNRYQWRTVTPVTFYNAGKTHYLIAIHQDTAKMF
                     TIERIQNFRFSRQASSIKNVPTLNELFRHSWGSFTGGKLTDVHLRFKDDLGQYMAEKF
                     WIEEQAMKQSDNGFEIRLKVKLSNEFIAWILGWGDAVTVLAPDELRQSVLEKAKSIVN
                     HYKK"
BASE COUNT         1684 a         1217 c         1378 g         1682 t
ORIGIN      
        1 ttcaaaagac ttctactgtt gcaatcgttc aaaaggcttt ccgatttttt gaagcaaaag
       61 gcatttcagc caacgaaatt gtaaaacgca ttcaagccaa tttcaaacct gaaaattgcc
      121 tcagtgccaa caatgacgca gaagctgagc aaaaccaaat attactcaac acattggaag
      181 atgaaaacaa cccaatccgt gccgtttttg ccgttcagaa actgaacgaa gggtgggacg
      241 ttttgaattt gtttgacatt gttcgcctgt atgaagatcg tgacggaaaa gacgggaaac
      301 cgggaataac cacgctttca gaagctcaat taatcgggcg aggagcgcgc tatttcccat
      361 ttgctataga agagggtcat gatgttttca cccgaaaatt tgatgatgac atttccaatg
      421 atttaaaaac gttggaagaa ttgtattatc acacaaaaga ggatagtcga tacatttcgg
      481 aattaaagaa ggcgcttgta gattcgggta tttatgaaga tgaagaaaat cttgtaacca
      541 aacagcttag acttaagatt gactttaaag aaacggagtt ttaccgagat ggtcacgtat
      601 tcttcaacaa gaaaattcta aaacgttctg ataatatcaa atcatttgcc gatttaggcg
      661 tcaaaagaac caactatcgt cacatacttt cttcgggtgt cgggagaatg tccagcgctt
      721 tttttgaaat ggagccgaca cagtcgggtg aagaaatcat taaaagtaaa gatgtaaagc
      781 tgactgatat tccgaaaaac acaattcgtt ttgcattaag tcaaaatccg tttttctact
      841 tcgacagctt gtcgtattac tttccgagtg ttggttcact ttccaatttt attgacagca
      901 aagaattgtt agcaagttta gaaatcacat ttaacggaac agctaaccgc ctgaaagaaa
      961 ttaaccattt cgattatctg caagctttaa acgggctttt gcaaagcatt gaagcagaca
     1021 taaaaagcaa ttcaactgaa tacgaagctt cggactatat caaagaacct attcacaaag
     1081 ttttcaaaga caaagaaatc aaggtttaca aagacagtgt acgagcagac gggcaagaaa
     1141 ctttagtagc caacgagcct tggtatgttt acaatgccaa ttatggaaca agtgaagaga
     1201 agaaatttgt agaacttttt tccagacgat ttgaaggact gaatttgaag tttgagaata
     1261 tttacttaat ccgaaacgaa agagagatca aaatctttga caaactcgga cgagcatttg
     1321 aacccgactt tttgctgttc tgcaaacaac gtgatggaga acaaatgact tttcaggttt
     1381 tcatagaacc aaaaggaaat catttgttaa gctatgacaa atggaaggaa gattttttaa
     1441 aggaaattag aaatgaacaa aaagctatca aaatccatac ggatacatat ttaataacgg
     1501 ccgttccatt ttataactat gataatgaaa acgaatttag aataacttta gaaaatactc
     1561 tcaaatagat ttagttatct aatcattcaa atgtacttta ttcggtagcc gatacttttc
     1621 acatagtcca ttcaattttt cttccaacgc agtcagataa taatcgggca tcgtagcaga
     1681 acggtcgaaa tacccacggt atttatccat taagtgcgga taatgctgtt gaatcgtccg
     1741 gagtgtgcgg attttgctgt cggagggctg atcaccgtac aaagtcaaag aagccggaaa
     1801 aatataccgt gccccgattt gtgcaaacgt cgaaaacgat agttcgagtt gatctgccgt
     1861 atcggatatc cacggtagca gcggcatcag actgacgccc gcgtaaaatc cttcatccaa
     1921 aatttgtttc agtccttgca ggcggcgcga cggcggtgtc gcaccgggtt cgaagatgtg
     1981 cgccgtgcga tcatctaccg acgaaaaaga aaatgtgatg agtgcgcccg acggtacttt
     2041 gcctaacaag tcttcgggca aaatagcagt cttaccgatg tgcatcaaaa tatcggtatc
     2101 gcgtaatatc agatcggaac gcgtgatgat gtgaaccgga aatcggtggt gcaagatgat
     2161 ctctaataat cggcgcgtga gcccgagttc cttttccgca tgtacatacg ggtcggtgga
     2221 ggaagcgagc acgatgaatc catattgtcc gcgtttggcg cgaaggctta gctgtttatc
     2281 cagcagttca ggcgcattgg ttttatagga taattttttt tcggtgtgtg taccgtatcg
     2341 gcttccatgt acataacaaa atacgcatcc catcgaacag ccgctgtatg gattgagggt
     2401 atagtcatcc aaaaaccacg gatcgcggcg tttggttttg ttgaggatgg ttcgaacagt
     2461 gacaggcgta cagggcataa aaataaaatt ctgtcaacgt atggtaattg gaaaaacacg
     2521 cgcgtattgg ttgagcatcc gttacgagga tatttttggt caggcgcaaa aacagcctgc
     2581 tgtcctataa tcgaacaaaa acaaaatcag ttttcgtctg tacctgtttt gtttttcaaa
     2641 agatgcttca aagccgaaac atccatttga tctctttcac ggagtgactg ttctttgaac
     2701 caaaccaaca tggaagcatc caaaaaagga attttgtgcc cgtccaaagc gtgaaaacga
     2761 atatgagagg tgagatcgga ataacgtttg ccgtttatcc gtgtaaaaat atctattgga
     2821 aaaatctcaa agaggcgaac ggcgccttct tcatcaggaa aatcttctac ggaaagttcg
     2881 cgggcgtacc cgtcatgata gccggataat atggatttta ttttttgtca gcaggaccaa
     2941 caatttttcg tattcgttca ttgttttcct gtttaggttt tttgccgatt ttttttagaa
     3001 attcattcag ctctgcgacg agttccgtag ggcgtttttt ggattggccg atttgcttac
     3061 ggatatgttc acccacaccc atgattatcc ccggatggtt tcgatcattt cgatgacgcg
     3121 ttgcaagtcg tcattcgaat aatattcgat ctctacggag ccgctgccgt ctttgtgatg
     3181 atgcaggcgc actttggtgc ctaacttcat acgcaggcgt ccttccacat ccgcgatatg
     3241 cgggttggcc ggttgcgcgg attcgatttc atatttattc ggcgaaactt tgtggctttt
     3301 tccttcgagc tcctcaagcg tcgttttgac caactgttcg gttttgcgaa cggacatgcc
     3361 gccgtccacg atctgcttcc atgctttgag ctgaagctgt tcggagggga gtgcgatcag
     3421 agcgcgtccg tggccgatgg tgatttcttc ttttttgagg ctttcctgga tatcggccgg
     3481 cagttttagc aaacgaataa tattggtaat ggtcgtacga tctttgccga ttttttgagc
     3541 gacctgatct tgcgtcagat tgcaggtttc gatcagcagc tggtaccctt cggccaattc
     3601 gatcggattg agtttttcgc gctggacatt ttcgatgatg gccagttcga gcatttcctg
     3661 atcggtgaca ttttcacgga tgtaagcggg taactcagtc agcccggcca atttgctggc
     3721 gcgaaaacgg cgctctccgg cgatcaattc ataaccggtc gccgttttac gaacggtgat
     3781 cggctgaatg ataccgtttt gcagaatcga ttgttttagt tcttccagcg cctgcggttc
     3841 gaattcctta cgcggctgaa aggggtttgc acgaatctgg tcaatcggta ttttagcgat
     3901 gggcgtatcg ctccgcactt catctttgag cgtaccgtcg gcattaagtt cttcgcgaat
     3961 aagcgcacta aggccttttc caagacccgt attttttttg ggcgtaaaat tcatataaaa
     4021 tttgtgttat gccggttgcg gcgtgatgac gttggcgatg acttctttgg ccacgcccat
     4081 atagtcttga gaaccgacgg acgcaggatc atagaggatg atcggtttac caaagctggg
     4141 cgcttcaccg agtttgatat tgcggtgaat gacatttttg aaaactttgt ctttgaaata
     4201 ttctttgact tcgcccacga cctgattgga caaattgagg cggctgtcgt acatcgtcat
     4261 cagcacgcct tcgatggtca gatcggtatt gaggtgacgc tggacgaggc gaatggtatt
     4321 gagcaactgg cgtaatcctt cgagcgcata atattcgcac tgaatgggaa tcagtaccgt
     4381 atccgcggcc gatagcgcat taagcgtgag aagaccgagc gacggcgggc aatcaatgag
     4441 aatgtattcg tactgatcac gaatcggagc aatggctttt ttgaacatgt gttcgcgttc
     4501 ttcgagattg accaattcga tctcggcgcc gacaaggcgg atgtgcgccg gaaggagatc
     4561 catactgggc atcatcgttt tcaaaatcgt atcggttgcc ggaacggttt gaatcagaac
     4621 ttcatacgaa ccgcgttcga ttttttcgag atcaaaaccg aggccggtcg tggaattggc
     4681 ctgcggatcc atatcaataa taagcgtgcg tttgccggca tgggcgaggc tcgcagaaag
     4741 attgatcgaa gtcgtcgttt taccgacgcc acctttttgg ttggctatgg caataattct
     4801 gcccattcat tctcctgaaa cgatgtggaa cactgtatga tgtttcacga atagcccgca
     4861 aagataggga agcgggttct atttagcaac gcatttttta taaaaatgcg agctattttt
     4921 aatcattata agatcatttt ttataatgat ttacgatgct ttttgccttt tcgaggacgc
     4981 tttggcgcaa ttcgtccgga gccaaaacgg tcactgcatc accccatccc aaaatccacg
     5041 ctataaattc attggacagt ttgactttga gtcgaatttc aaacccgtta tcgctttgtt
     5101 tcatcgcctg ctcttcaatc caaaattttt cggccatgta ttggcccaga tcatctttga
     5161 aacgcagatg cacgtcggtc agtttaccgc cggtaaaaga tccccaggaa tggcgaaaca
     5221 gttcgttgag cgttgggaca tttttgatgg aggatgcctg ccgcgaaaaa cgaaagttct
     5281 gaatgcgctc gatggtaaac attttagccg tgtcttggtg tatcgcgatc aggtaatgcg
     5341 tttttccggc attgtaaaac gtaaccggag tgacggtacg ccactgataa cggttggttt
     5401 tagcagaacg gtattcgatc tcgatgacat gcttttggcg tatcgcgcgg atcgtttcaa
     5461 cgaagaaact caacgttttg ttttgaaatc gctccaaaac gagcggaaga tttttgatcg
     5521 tttcattatt cccgaaagcg aaatatgtcg tgatgagcgt attgatctgc aaaagggata
     5581 gttcgatgcg aaacgcgcgt ttgcgggaat tgacggtgat gcccatcgcc cgaagtgcac
     5641 gcatatcgcg gtgaatggag gattcgctgg tatgaaattt ttcggctaag tctatttcgg
     5701 aatatttgtc ggggaattca taaatcagcg cggcgatttc gatctgacgc tgaagatcat
     5761 caatactatt aatcatgcta cgcatcccaa taaaaattaa tcgttgaaat gtcattaaaa
     5821 ataatattga aatcaaatat tgtcaaaaat tgacagaaaa gggccgccaa cagactataa
     5881 attaataacg ttacgatgat gaaaacgaaa cgaaaaagaa tttactaata acacataaaa
     5941 acaaaacaag gagaacaagt c
//
LOCUS       DAOQNQ010000200         5956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_5244_length_5956_cov_6.887985, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000200 DAOQNQ010000000
VERSION     DAOQNQ010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5244_length_5956_cov_6.887985"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2296)
                     /locus_tag="PK754_11490"
     CDS             complement(<1..2296)
                     /locus_tag="PK754_11490"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB10172.1"
                     /translation="MKITISRKLLLVLATGVTVLAGYFYVDMRREQNFLRSNEPVLSD
                     TTQARWWFEEGVSLTEKSRYDSSLIFFERATGIYQRDSLWQFYIDGMNYIGDNFRRLG
                     RYDTAFSILTANIDRSIRYLGETDVSTAMAINKMGLWYMDQGDDEKAKNNFNRALWIR
                     KKTLPGDHIDIGWSYNNLGLVNYNSGYFTLALQYYDQALGVFMRTIGENSGPVAVLNN
                     NMASVYQAQGNHEKALEHINRSLQIRKTLFGSDHVTLSENYTLMGTAYLRKGEMGQAF
                     KLYNQALSVNERKYGLGHPTVGNNYYNLGFAFKEVRQMDTALSYYRYALNNWKKNYGL
                     KHPVIARVSDEIGELFLEKNMYDSALFYFNNALTIWNNRPGAKGFRRAHEYSHIGMTY
                     LKMGDVKKSLTYYEQALSINLEYYGSKHLEVSLCYSNMAGAHRIVKDYPAALSNIQKA
                     IDALLIADSASGFMYRTRDAVKLMLALELKGDILQEYYEYKTRNLQHLKASFASYAMA
                     VEWLDRTRREYVSEDSKILIGQRSIGLFEKAIQTATDLYKITSEKKYLNEAFIYSETS
                     KAAVLSEVVSRQHAKRFAGIPDSLLEYEEQLRNRIGVYNKKLFSEQNSGGYGSAKFRY
                     IQDELFQLTSEYEAYLKHVEQTFPQLYSLKYKPLIMPLEAVQKLIPDSTVLLEYFIGN
                     RHLYVFCVTRSGCAWAAAPVDSSLIGVVHALTAGIQTMNMALYSTSAHYLYNVVFKPF
                     EKTSAVKLIVVPDGILSGIPFEVLL"
     gene            complement(2482..2958)
                     /locus_tag="PK754_11495"
     CDS             complement(2482..2958)
                     /locus_tag="PK754_11495"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNB10173.1"
                     /translation="MKKWVMQWLVVVILCGPVIFAQDKPKIAVVPFKNNTGVKDYDGI
                     AVGIGESITTVLSGSGKFDFIERIQMDKAIQEQDFQMTDHVDPKTVQEIGKILGVQYM
                     IIGDLQKIKDKFRINARRMRVEDGKLEEAVSVTGIEDELFDMQDAIARKLDERFSP"
     gene            complement(2979..5078)
                     /locus_tag="PK754_11500"
     CDS             complement(2979..5078)
                     /locus_tag="PK754_11500"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNB10174.1"
                     /translation="MNSRISYRICFDRIQKWLLLVLTSLSLEIVLYAQAPVSRGVAVL
                     TFDNLTGDASNDWIGAGFGETMTNKLNNVNALRMQGRRQLNDLLLTRGWDQKKIRENQ
                     AEEVGRLLGVDYLLIGSVQAAADISNPEAELYVNARIVDVHTAQILQTLAIRGKMMNL
                     LGLQTALAMEFIRKLGVETTVSEQNALAYEETKSLEAYRYYAMGNDFLDQTRYKEAIE
                     MYKLAMAKHGGIFYAEANHNKVEAYLRLAAQSGDARDSVLTQLVHGEKKKVSELAPIF
                     FNLAQAEELNGNFDKAAEAYRTFIDYCDSKLLRWKFTSGKAIVAAPKTYQENMVFTAS
                     DNYVYNVQSVSGQLKWKNKISDKPQSEPFIFEGKIYLADEDENLFAVHALHGQLIWTK
                     HISGLTKAAPWVSDGLVVVGLRTGHVAAFRSAGGDPVWKTDIASPILGSTAGRDAKIY
                     VGTQDGTIACLDQKTGRLLWKTRLEGTIPTKVSPDKTMLFAVTEQGFVVALDPVSGQP
                     VWKTNVDGKVTQPAAWSDKDVFVVTEKGTIVALNKADGVIRWTYQSRAKQLTAPQWIE
                     QLVYIGADDHNVYAIHSQTGKLAWRYETNAEIASEPMILSGVVYVGTGDGGVYAISEK
                     KDPAQHSDLDAYLSLGRVATYQRKIEDALRIYEYITQYVKVNVPEAYIEMEKLYREKG
                     DEAKAKEMKKKYIQSRN"
     gene            complement(5124..>5956)
                     /locus_tag="PK754_11505"
     CDS             complement(5124..>5956)
                     /locus_tag="PK754_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10175.1"
                     /translation="SSDTDKKYVYAFRITNMKKPLLLYSDLDARFQHAYCLLKSGDLA
                     AAEMEARELLELKNDYLPAYYLMSLVHRVRGNAAEELAALHEYYLRGNFEVVKERVYE
                     LSGIKQIYRFPKHEKAYLAKKGSSIFLKIIFGTTTDNEVYELKNEAVLYTKVSHSAGP
                     WGDTYNEKGNPAIDSVRSTEYSFNYGGKFQYNSWVKANGYGNAETTPFTAYQGQQIKW
                     RLDFLTPYYDGWRNGNRGCYLNATGDLLYISAGDGLVHAVDLKKVDTLIGQGQKWWKE
                     "
BASE COUNT         1541 a         1473 c         1174 g         1768 t
ORIGIN      
        1 gcagtaacac ttcaaacgga atacccgaga gaataccgtc cggcacgaca atcagtttta
       61 cggcgcttgt tttttcgaat ggcttaaata caacattata caaataatgc gctgaagtcg
      121 aatacaatgc catgttcatc gtttgaatcc cggcagtcaa cgcatgcacg actccaatca
      181 gcgatgaatc aaccggcgcc gcggcccatg cgcatcccga tcgggttacg caaaacacat
      241 acagatgccg atttcctata aagtactcca ataagaccgt cgagtcgggt atcaattttt
      301 gaacggcttc gagtggcatg ataagcggct tatatttgag agaatataat tgcggaaaag
      361 tttgctccac atgcttgaga taggcttcat attccgaggt caattgaaat aactcgtcct
      421 gaatatatct gaatttagcg ctgccataac cacccgagtt ttgctcagaa aaaagttttt
      481 tattatatac accgatccgg ttgcgcaatt gttcttcgta ttccagcagt gaatcgggaa
      541 tgccggcaaa tcgtttggcg tgctggcgac tcacgacttc ggacaaaaca gccgctttgc
      601 ttgtttccga ataaatgaag gcctcgttga ggtatttttt ttcagaggtt atcttataaa
      661 gatccgttgc cgtttgaatg gctttttcga aaagccctat agaacgctga cctattaata
      721 ttttagaatc ctcggataca tattcccgcc gcgttcgatc taaccactct acggccatgg
      781 catacgacgc aaacgacgcc ttgaggtgtt gtaggttccg ggttttatac tcataatact
      841 cctgcaaaat atcacctttc aattctaacg ccagcatgag tttgactgca tcgcgcgttc
      901 ggtacataaa ccccgatgcc gaatcggcga tcaaaagcgc gtcgatagct ttctgaatgt
      961 tgctcaaagc cgcaggatag tcttttacaa tccggtgcgc accggccata ttggaataac
     1021 aaagggaaac ttctaaatgt ttgctcccat aatattctaa attaatagac aatgcttgtt
     1081 cgtaataggt cagacttttt ttgacatcac ccattttcaa atacgtcata ccgatatgac
     1141 tgtattcatg tgctctgcga aaacctttgg caccgggtct gttattccaa atagtaagcg
     1201 cgttattaaa gtaaaaaagc gcgctgtcat acatgttttt ttccagaaat aattcgccga
     1261 tttcgtcgga tactctcgcg ataaccggat gcttcagtcc gtaatttttt ttccaattat
     1321 ttaaagcata acgatagtag gacaatgccg tatccatttg acggacttct ttgaaggcaa
     1381 aacctaaatt gtagtaattg ttcccaaccg tcgggtgccc cagaccatat ttcctttcgt
     1441 tgacagataa cgcctgattg tacaatttga acgcctgacc catctcacct tttcgcaaat
     1501 aggcggtccc catcaacgta taattttccg ataacgtgac atggtcgctt ccaaaaagcg
     1561 tttttcttat ttgtaatgat cggtttatat gctccaacgc tttttcatga tttccctgcg
     1621 cttggtaaac gctggccata ttattattta atacggcgac ggggccgctg ttttctccga
     1681 tggttctcat aaaaacaccg agcgcctgat cgtaatattg caacgccaac gtaaaataac
     1741 cgctgttata gttgacaagt cccaggttat tgtaactcca tccgatatca atatgatcgc
     1801 cgggaagtgt tttttttcgt atccataagg ctctgttgaa attgtttttg gctttttcgt
     1861 catcgccttg atccatgtac cacagaccca ttttattgat tgccatcgcc gttgatacat
     1921 cggtttcccc caaataccga atactccgat ctatgttggc cgtgagaatg gagaaagccg
     1981 tatcgtatcg gccaaggcgg cgaaaattat cgccgatata attcatacca tcaatataaa
     2041 attgccacaa actgtcgcgt tgatagatac cggtcgcacg ctcaaaaaaa atcaaggaac
     2101 tgtcgtacct acttttttct gtcaaagaaa cgccttcttc gaaccaccat cttgcttgtg
     2161 tggtatccga taatacgggt tcatttgacc gtaaaaaatt ttgctcacgc cgcatatcaa
     2221 cataaaaata acccgccaaa acggttacgc ctgttgccaa tacaagaaga agcttacgcg
     2281 aaatggtaat tttcatatat ccgatggatt ggataaaata gcggttaaac cagtggaaaa
     2341 atataggtgt gattctatat atagaaaacg ggttaagcaa tatcagaatt tatccctgtc
     2401 ggcacgggcg ataaaatctc aagtgtgtca ctcaacatca atttccatca tctctaatac
     2461 gtgaggtatg tgattttatt tctacggact aaatcgttca tccaactttc gcgcaatcgc
     2521 atcctgcata tcaaaaagtt catcctcaat accggtgaca ctcacggctt cctccagttt
     2581 cccgtcctct acacgcatgc ggcgagcatt gatacgaaat ttatctttta tcttctgcaa
     2641 atcgccgatg atcatgtatt gtacaccgag gatttttccg atttcctgga ccgttttggg
     2701 atctacatgg tcagtcatct gaaaatcctg ctcctggatc gctttatcca tctggattcg
     2761 ttcaataaaa tcaaatttgc cgctccccga taaaacggtc gtgatacttt caccgatgcc
     2821 taccgcgata ccgtcataat ctttaacacc cgtattattt ttaaacggaa caacggcaat
     2881 ttttggtttg tcctgtgcaa aaatcaccgg tccacaaagt atcaccacaa cgagccattg
     2941 catcacccat tttttcataa accactctcg tttgtatatt agtttcgtga ttggatgtac
     3001 ttctttttca tctctttagc tttggcttca tcgccttttt cacggtacag tttttccatt
     3061 tcaatatatg cttccggcac attgactttg acgtattgcg tgatgtattc gtagatacgc
     3121 agagcgtctt cgatttttcg ctgataggtc gccacgcgtc cgagcgacag atacgcatcc
     3181 aaatcactat gctgcgcagg atcctttttt tcactgatcg cataaactcc gccatcgccg
     3241 gtgcctacat atacgacgcc acttaaaatc atcggctcgc tcgctatctc agcattcgtt
     3301 tcatagcgcc atgctaactt tccggtttga gaatgaattg catacacatt gtgatcatcc
     3361 gcaccgatat aaacaagttg ctcgatccac tgcggagcgg ttaactgttt agctcgcgat
     3421 tggtacgtcc atcgaatgac accatctgct ttattgagtg ctacgattgt tcctttttca
     3481 gtcactacaa atacatcttt atcactccat gccgcgggtt gtgttacttt tccatccaca
     3541 tttgttttcc aaacgggttg accactaacc ggatcgagtg caacgacaaa cccttgttcc
     3601 gtgacagcga acagcatcgt tttatccggg ctgactttag tcggtatcgt accttctaat
     3661 cgtgtcttcc ataagagtcg cccggttttt tgatcaaggc aagcgatcgt accgtcctga
     3721 gtgcctacat atattttagc atctcgtccg gccgtactgc caagtatcgg agaggcaata
     3781 tccgttttcc aaaccggatc accacccgcg cttcgaaacg cggctacatg tcctgtacgc
     3841 aagcctacaa cgacaaggcc gtcggatacc caaggcgccg ctttggtcaa accggagata
     3901 tgtttggtcc atataagttg accgtgaagc gcatgaacgg caaaaagatt ttcatcttca
     3961 tcggctaaat agattttgcc ttcaaagata aatggttcac tttggggttt atcgctgatt
     4021 ttatttttcc actttagttg ccccgacacg ctttgcacat tatagacata attatccgac
     4081 gctgtgaaaa ccatgttttc ctgataggtt tttggcgctg cgacgatcgc tttcccggat
     4141 gtaaatttcc accgaagaag tttagaatcg caatagtcaa tgaatgtccg gtaggcttcc
     4201 gccgctttgt cgaaattgcc gttcagttct tcggcctgcg cgagattaaa aaatataggc
     4261 gccagttccg aaactttttt cttttcgcca tgaaccaatt gggtcaatac ggaatcccgt
     4321 gcgtcgccac tctgcgccgc caggcgaaga taggcttcca ctttgttgtg atttgcttcc
     4381 gcataaaaga tgccgccatg cttggccatc gccagtttat acatttcgat cgcttctttg
     4441 tagcgtgttt gatcgagaaa atcattgccc atggcataat aacgatacgc ttccagcgat
     4501 ttagtttctt cgtacgccaa agcgttttgc tcgctcaccg tagtctcgac gcccaacttt
     4561 cggataaact ccattgccag cgctgtctgt aacccgagca gattcatcat ttttccacgg
     4621 atcgctaacg tttgaaggat ctgcgccgta tgcacatcca cgatacgcgc attgacatag
     4681 agttccgctt ccggattgct gatatcggcc gcggcctgga ccgaaccgat cagcagataa
     4741 tctacaccga gcaatcggcc tacttcctcc gcctggtttt cccggatttt tttctgatcc
     4801 catccacgcg tcaaaagtaa atcattgagc tgacgccggc cttgcatacg aagtgcattg
     4861 acattattca gtttattcgt cattgtttcg ccaaaccctg cgccaatcca atcgttggac
     4921 gcatcgccgg tgaggttatc aaatgtcaat acggcgactc cacgagagac cggggcctga
     4981 gcatacaaca ctatctccag gcttagactg gtcaaaacca acaacagcca tttttgtatt
     5041 cgatcaaagc agatcctata acttatgcga ctgttcataa gttccttttc aaatttatcg
     5101 tgcatgacat ttttttcgcc acattattcc ttccaccatt tttgcccctg gccgatcaat
     5161 gtgtccactt tcttaaggtc aaccgcgtgt accaatccgt cgcccgccga tatatacaaa
     5221 agatcaccgg tggcattgag gtagcaaccg cgattcccat tgcgccaacc gtcatagtaa
     5281 ggcgttaaaa aatctaaacg ccacttgatc tgttgacctt gataagcggt gaaaggtgtt
     5341 gtttcagcat ttccataccc gtttgctttt acccaactgt tgtattgaaa tttgccgcca
     5401 tagttaaaag agtattctgt agatcgaaca gagtctattg cagggttacc tttttcatta
     5461 taggtatctc cccacggtcc ggcgctgtga cttactttgg tgtagagcac agcctcattt
     5521 ttcaattcat acacttcatt atccgtggtt gtgccaaaga tgattttaag aaaaatggac
     5581 gaaccttttt tagccagata tgctttttca tgtttgggga agcgatagat ctgtttgatg
     5641 ccggacaatt catacacccg ctcctttaca acttcaaaat tccctcgtaa ataatactca
     5701 tgcagtgcgg ctaattcttc cgccgcatta ccgcgcaccc gatgtaccaa tgacatcagg
     5761 taatacgcag ggagataatc gtttttcagt tcaagtaact cacgcgcttc catttctgcc
     5821 gccgccaagt cgccgctctt caggagacaa taggcatgtt gaaaccgtgc atccagatcg
     5881 ctatacaata aaagcggttt cttcatattg gttatgcgaa acgcataaac atatttttta
     5941 tccgtgtctg aacttt
//
LOCUS       DAOQNQ010000201         5953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_5251_length_5953_cov_6.656324, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000201 DAOQNQ010000000
VERSION     DAOQNQ010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5251_length_5953_cov_6.656324"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="PK754_11510"
     CDS             complement(<1..888)
                     /locus_tag="PK754_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HNB10176.1"
                     /translation="MNTLHNDSFQRRHIGPRAEDLKSMLQAIGVGSLDELIEKTVPPS
                     IRLTSPMNLPPAIGEYEMLAELRALAAKNKIYKSYIGQGYYDTITPGVILRNILENPS
                     WYTQYTPYQAEIAQGRLEALLNYQTMVMDLTKMEIANASLLDEGTAAAEAMHMFHASA
                     NEGTGNKFFVASSCFAQTIDVMKTRANPLGIEIVVGDHTTFKPSKEYFGALIQYPAAD
                     GAIYDYAAFTQQCHEQGVYVAVAADILALTLLTPPGEWGADVVVGSTQRLGIPMGYGG
                     PHAAFFATKDEFKRKMPGRI"
     gene            complement(925..1962)
                     /locus_tag="PK754_11515"
     CDS             complement(925..1962)
                     /locus_tag="PK754_11515"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB10177.1"
                     /translation="MSRTYKILIVDDNPDHVYSLRKTLQRPEYVFFEAHSGEDALGSV
                     TAHDPDIMLLDVRLPGIDGFEICESIRKISAHLPIVFVTANLKEFVDQVHGFEKGGDD
                     YVIQPYDPRELAIKVKALLRNKVLYDSLLQEVNKLDRMKAELQQHNEELKKVNNKLEE
                     KTEHLKSLTVTDPLTALFNRKYFHQRINKEISAVKRYKAEASAAMVDIVNYTGINEQY
                     GVPQADVILKEMASLLVNSVRNSDIVTRFDGGKFAVIFTHTPEDSALIKARMIVEAVA
                     AYPFPIYDDLIPQEKINELDKKEIRIRAKISLTGLNHDWVRTEADLIEAFERSLLQNR
                     SNQDYVFFARK"
     gene            complement(1959..4169)
                     /locus_tag="PK754_11520"
     CDS             complement(1959..4169)
                     /locus_tag="PK754_11520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB10178.1"
                     /translation="MKSRKKTSLKKRSSQKTVASRTAKPVQSKSKKKSPKAIKKSKPR
                     SSSSVILSAQKTSASVITPMIRALLDSAASGILVIDHQRHIVAVNAKFCDLFNADEPT
                     LTLWKESDLVMRFTSLVRESERFTKERDLIDNENYIVSDEIEFTHMSGRFFLRQILPI
                     PDERGKIVGRLWKFRDVTYERHIAVVYRDIEKKKFEFEEKNQELENAYRRLEEMRRDV
                     VEANQLKSQFLSNMSHELRTPLNSIMALSSILLARMDGELTEEQEKQIRIIEKSGKNL
                     LALINDILDLSKIESGKMDVFFAEYAVREFADSIRMTVLPMVRESNLEMEVDVDETLD
                     IHSTDENKLKQILLNLLSNAIKFTPHGKVRLQVRPTKFADVLEFSVIDTGIGIDRENY
                     EKIFDPFRQLDGSATRKYGGTGLGLAVTKRMVELLGGKISVESEIGKGSTFKFILPAK
                     KRGDSSKPFSAQEIEAMFTRKQDEADQLITDSAINPAKKTVLLVDDDKETLYAFRKYF
                     EEEHYNILFAQDGETALAKSAQYRPDVITLDIMMPRKDGWEVLQELKKNPDTKNIPVA
                     IVSMIDNKKLGYSLGTSDYLVKPVQRDAFMKRIQKLTEMKGLRKILIVDDDLSQAELI
                     EEILESDDFLSEVATSGEKAIQAARRKSYDLVILDLMMPQIDGFAVLSSLQSDPITQN
                     LTVLVLTGKLLTQEDHRKLSGNHYYIFQKSAFSREKLLEQIHRIMEAERNATKP"
     gene            complement(4194..4574)
                     /gene="gcvH"
                     /locus_tag="PK754_11525"
     CDS             complement(4194..4574)
                     /gene="gcvH"
                     /locus_tag="PK754_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNB10179.1"
                     /translation="MNFPEELLYAASHEWVRLEGDIATVGVSDYAQSELGDIIFLELP
                     EVGKKVTAGQPFGSVEAVKTVSDLYAPFSGEVIEVNGVLADKAETINSDPYGNGWIVK
                     IKVSDMSAKSSLLSANKYRESTAH"
     gene            complement(4636..>5953)
                     /locus_tag="PK754_11530"
     CDS             complement(4636..>5953)
                     /locus_tag="PK754_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10180.1"
                     /translation="TAPSVKKEIPKPEEKSASQPKVVKRPPERKTDDYKHEVHKDDTI
                     HRHVDSAFGSGEDTGVFITPEQETAFFEKASAEDNEVVPYSEVADQDILTAEEQEAEL
                     DELEMSYKDYLDVLTDEADLEEALFKDDEEESVVADDETDAITERLAKVQAAPDDDFI
                     DEEDAPMSYSDYLECVESDGERDEALLDDDNEIMRFAAFAEWMDASDDTIDYATYQMM
                     EGLEEQTPKNVASTESVITEEEPAISYAAYVAGLSDEADRAEARLDAVTEDVPKAAQT
                     AAIIPEKAEEKPVPEIKAVEEPVKQPEMPQTEAFVTELKQIDTPKAEAKSLVAEVPPI
                     MTVVQETPKPVVEAVKEETAKPAAVATTEEEAEVVTEEVEITEEDINPQDATMEMVEK
                     FAQAGQFGMSYKVCKMLKMKNPTDAKVDRKVLELKRLYLWSSQLVG"
BASE COUNT         1413 a         1466 c         1319 g         1755 t
ORIGIN      
        1 aatacgacca ggcatcttgc gtttaaattc atctttcgtc gcaaaaaaag ccgcgtgcgg
       61 tccgccgtaa cccataggaa tacccaagcg ctgcgtggaa ccgacaacga catccgcacc
      121 ccattcaccg ggcggtgtta acaacgtaag cgccaatatg tccgccgcta cggcgacgta
      181 aacaccttgt tcgtgacatt gctgtgtaaa tgcggcataa tcatagatcg cgccatccgc
      241 tgccgggtat tggatcaacg cgccaaaata ttctttcgaa ggtttaaacg tcgtatgatc
      301 gccgaccacg atttcgatgc ccaaaggatt ggcgcgtgtt ttcatcacat ctatcgtttg
      361 cgcaaaacac gacgaggcaa caaaaaattt attgccggta ccttcattgg cggaagcatg
      421 aaacatgtgc atggcctccg ccgccgccgt accctcatcc aagagcgaag cgttggcaat
      481 ttccattttg gtgaggtcca tgaccatggt ttgataattc agcaatgctt ccagacggcc
      541 ttgcgcgatt tcagcctgat acggcgtgta ctgtgtgtac caactcgggt tttccagtat
      601 gttccgcaga attacgccgg gcgtgatcgt atcataatag ccttgcccga tgtacgactt
      661 gtaaatttta ttttttgccg ctaaagcacg caattcagcg agcatctcat attcaccgat
      721 ggccggaggc aaattcatcg gcgaagtgag acgaatggaa gggggaacgg ttttttcgat
      781 cagttcatcc aaggagccca caccgatggc ctggagcatg gacttcaaat cctctgcgcg
      841 tggtccgata tgacgccttt ggaaactgtc gttatgcaac gtattcatat taactcctta
      901 tgggaaaatt cgatataagc gggattactt gcgggcaaaa aatacatagt cttgatttga
      961 gcggttttgc agtaaggatc gttcaaaagc ttcaatgaga tcggcttccg tgcgcaccca
     1021 gtcatgattt aatccggtca acgatatttt tgcacggatt cggatttctt ttttatccag
     1081 ttcatttatt ttttcttgtg gaataagatc atcgtaaatg ggaaaagggt atgcggctac
     1141 ggcttccacg atcatacgcg ctttgatcaa cgcactgtct tcgggcgtgt gcgtaaaaat
     1201 aacggcaaat ttaccgccat caaagcgcgt cacaatatcg gaattacgga cgctattgac
     1261 caacaggctg gccatttcct taagaatgac gtcggcttgc ggtacaccgt actgttcgtt
     1321 aatgccggta tagttgacga tatctaccat cgccgcgctg gcttcggctt tgtagcgttt
     1381 gaccgcgctg atttccttat taatgcgttg atgaaaatat ttacgattga aaagcgccgt
     1441 gagcggatcc gttaccgtaa gcgacttcag atgttcggtc ttttcttcga gcttattatt
     1501 tacttttttt agttcttcgt tatgctgttg aagctccgct ttcatgcggt cgagtttatt
     1561 gacttcttga agcaaactat cgtaaagaac tttattgcgc aggagcgctt ttactttgat
     1621 cgccagttca cgcggatcgt aaggctggat cacataatca tcaccgcctt tttcaaaccc
     1681 atgaacctga tcaacaaact ctttaagatt ggccgtaaca aaaacaatgg gcagatgcgc
     1741 ggaaattttt cgaatacttt cacaaatctc aaatccgtca atgcccggca aacgtacgtc
     1801 cagcaacatg atatcgggat cgtgcgccgt tacggagccc aatgcatctt cgccgctgtg
     1861 cgcttcaaaa aacacatact ccggccgttg caacgttttg cgcagggagt acacatggtc
     1921 gggattatcg tcaacgatta agattttata cgtgcggctc atggttttgt ggcgttacgc
     1981 tcagcctcca tgatgcggtg aatttgctcc agtaattttt cgcgcgagaa tgcacttttc
     2041 tggaaaatat aataatggtt acctgaaagc ttccgatgat cttcctgcgt aagtaatttg
     2101 ccggtcaata cgagtaccgt taaattttgc gtgatcgggt cactctgtaa actcgataaa
     2161 acggcaaagc cgtcaatctg cggcatcatc agatcaagaa ttaccaaatc gtaggatttg
     2221 cgtcgggccg cctggatggc tttttcaccg ctggtcgcga cctctgacaa aaaatcatcg
     2281 ctctccaaaa tttcttcgat cagttcggcc tgtgacaagt cgtcatccac gatcaaaatt
     2341 ttacgcaatc ccttcatctc cgtcagcttt tgaatgcgtt tcataaacgc atcgcgctgt
     2401 acgggcttta ccaaataatc cgacgtaccg aggctgtatc cgagtttttt attgtcaatc
     2461 atcgaaacga tggccaccgg aatattttta gtatcgggat ttttcttcag ttcctgcagc
     2521 acttcccaac cgtctttgcg cggcatcatg atatccagcg tgataacgtc cggacgatat
     2581 tgcgccgatt tggccaacgc cgtttcaccg tcttgcgcga aaagaatatt gtaatgttct
     2641 tcttcaaaat atttccgaaa cgcatacagc gtttctttat catcatccac caaaagcaca
     2701 gtttttttcg ccggatttat cgcactgtca gttatgagtt ggtccgcttc atcttgcttg
     2761 cgggtaaaca tggcctcaat ttcttgcgca ctaaaaggtt tgctcgagtc accgcgtttt
     2821 ttggccggaa gaataaattt gaacgttgaa ccttttccga tttcgctttc gacggaaatt
     2881 ttaccgccga gcagttccac catacgtttg gttacggcaa gtcccaaccc ggtgccgccg
     2941 tattttcggg tggccgatcc gtcaagttgt cgaaagggat cgaaaatttt ttcgtagttt
     3001 tcgcgatcaa tgccgatacc cgtatcaatt accgagaatt cgagaacatc ggcaaatttg
     3061 gtggggcgaa cttgcaatcg cactttgccg tgcggagtga acttgatcgc attggataaa
     3121 agattgagca gtatttgttt gagtttattt tcatcggtgc tgtgaatgtc cagtgtctca
     3181 tcaacatcca cttccatttc caggttggat tcacgcacca tcggcagaac ggtcatgcga
     3241 atactgtcgg caaattcacg aacggcgtat tctgcaaaaa atacatccat tttgccggac
     3301 tcgattttgg aaagatccaa gatatcgttg atcaaggcca gcaaattttt accgcttttt
     3361 tcgataatgc gaatttgttt ttcctgttcc tcggtcaatt caccgtccat acgcgccagc
     3421 agaatgcttg ataatgccat gatggaatta agcggcgtgc gcaattcgtg gctcatattg
     3481 gaaagaaact gggacttgag ttggttcgct tcgacgacat cgcgtcgcat ttcctcaagg
     3541 cggcggtatg cgttttcgag ttcctgattt ttttcttcaa actcgaattt cttcttttca
     3601 atatcacggt acacgaccgc aatatgccgt tcatacgtga catcacgaaa cttccaaagg
     3661 cggccgacga tttttccccg ttcatcgggt atcggtaaaa tctgccggag aaaaaaacga
     3721 cccgacatgt gtgtgaattc gatttcatcc gatacgatgt aattttcgtt gtcaatcaga
     3781 tcacgttctt tggtaaatcg ttccgactcg cgaaccagcg atgtaaagcg catcactaaa
     3841 tccgattctt tccataacgt caacgtcggt tcgtccgcat tgaataaatc gcaaaacttg
     3901 gcattcacgg ctacgatatg gcgttgatga tcaatcacca atataccgga tgccgccgaa
     3961 tccaataatg cgcgaatcat cggtgtaatt accgaagcag acgtcttttg tgctgaaaga
     4021 attacggagg aagaagagcg gggtttgctt tttttaatcg ctttaggtga tttctttttg
     4081 gattttgatt gcacgggctt tgccgtacgc gaggccacgg tcttttggga agatcgtttt
     4141 ttgagcgacg ttttttttct agatttcata gtgcaaagcc gtgtgaagtc cgattaatgc
     4201 gctgttgatt cgcggtattt atttgccgat aaaagacttg atttagcgct catatcggaa
     4261 actttgattt ttacaatcca accgttacca tacggatcgc tgtttatcgt ttcagctttg
     4321 tcggccagta cgccgtttac ttcgatcact tcacccgaga atggtgcata cagatcgctt
     4381 acagttttta ccgcttcgac ggaaccaaag ggctgacctg cggtaacttt tttgccaact
     4441 tcagggagct caagaaaaat aatatcgcct aattcagatt gcgcataatc agacacaccg
     4501 acggtagcta tatcgccttc gagacgtacc cattcgtggc ttgctgcgta aagtagttct
     4561 tcgggaaaat tcatgacaag actcctatat aaacattgat tcagggggca acggaaatta
     4621 ccaatccacc ataagctaac cgaccaactg actgctccaa aggtacaggc gcttgagttc
     4681 cagcactttg cgatccacct tggcatcggt cgggtttttc attttcagca ttttgcaaac
     4741 tttataggac ataccaaact ggcccgcttg agcaaatttt tcaaccatct ccatcgtcgc
     4801 atcttgcggg ttaatgtctt cttcggtgat ttctacttct tcagtgacaa cctccgcctc
     4861 ttcttccgtt gtcgccacgg cggccggttt tgcagtttct tcttttacag cttcgactac
     4921 cggtttaggc gtttcctgca cgacggtcat tatcggaggc acttcagcaa caagtgattt
     4981 agcctcggcc ttcggcgtat ctatttgctt cagttctgtt acaaatgctt ccgtctgagg
     5041 catctccggt tgtttgaccg gctcttccac ggctttaatc tcaggaaccg gcttctcttc
     5101 cgctttttcc ggaatgattg cagccgtttg tgcagcttta ggtacatcct ccgtaaccgc
     5161 atccaaccgt gcttccgcgc gatccgcttc atcacttagg cctgcaacgt atgcggcata
     5221 ggatatcgcg ggttcttcct cggtaataac actttcggtt gaggcaacat ttttcggcgt
     5281 ttgctcttcc aaaccttcca tcatctgata cgtcgcgtaa tcaatcgtat catcgctggc
     5341 atccatccat tcggcaaagg cggcaaaacg cattatttca ttatcatcat ccagcaaggc
     5401 ttcgtcgcgc tctccatcgc tttcgacgca ttcgaggtaa tcgctatagc tcatcggcgc
     5461 gtcttcttcg tcaataaaat catcatccgg tgcggcctgg actttagcca gacgttccgt
     5521 gatcgcgtcg gtttcatcgt cggctacgac ggattcctct tcatcgtctt tgaaaagcgc
     5581 ttcttcaaga tcggcttcat ctgtcagtac gtccagatag tctttgtaag acatttccag
     5641 ttcatcgagc tccgcttctt gttcttcggc tgtcaatata tcttgatcgg ctacttcgga
     5701 atagggtacg acttcattat cttcagccga tgctttttca aaaaaggccg tctcctgttc
     5761 cggcgtaata aacacacccg tatcttcacc gctgccaaat gccgagtcca catgacgatg
     5821 aatggtatca tccttatgta cttcatgttt gtagtcgtcc gttttacgct caggaggccg
     5881 tttgacgact ttgggctgcg atgcggattt ttcttcgggt ttgggaattt cttttttaac
     5941 agatggcgcc gtc
//
LOCUS       DAOQNQ010000202         5944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_5265_length_5944_cov_7.340126, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000202 DAOQNQ010000000
VERSION     DAOQNQ010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5944
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5265_length_5944_cov_7.340126"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..640
                     /locus_tag="PK754_11535"
     CDS             182..640
                     /locus_tag="PK754_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019990725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNB10181.1"
                     /translation="MRHNIKILIASVLFLACNNPEHKISAQSENEKIVRQYYEYFNNH
                     AWEKMAGMYTEVSEFKDPSLGPGIVKQTRQQIIQKYTELSTMFPDVKDEIIQTYPSGD
                     KHIIVEFVSTGTAPDNTKLQLPICTVFTIENGLISKDFTYYDNFETKTTN"
     gene            803..1972
                     /locus_tag="PK754_11540"
     CDS             803..1972
                     /locus_tag="PK754_11540"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNB10182.1"
                     /translation="MKNAMYGLLWSWILVSVVHGQTIDKYKLDVLLKKAEQTHSEAVI
                     VYKDNQLVTEKYFGIGQADTKIESMSCTKSIVGLAVACMLSDQTLASLDEPVSRYYPE
                     WRQGQKQAITVRHLVNMTSGLQNHPNASVEIYPSPDFIQLALAAELTSKPGEVWSYNN
                     KSLNLMAGVIKKITGQRMDQYIGERLFKPLGITDFSWSLDSAGNPHVMSGCQIKPGDF
                     AKIGLLLLNNGKYNNQTVIAEKYIQEVITPCKPYPGYGILWWIDYEKTTSIVDDEIVR
                     ELQKAGVAEDFIQKAKAMQGTYANNDAYYAKLLSVFGANPWEYINTTLGANLRLRKKE
                     FSGHVTYRADGYLGNYIIVDFKTKIVAVRMISHQSFQDETDNFNDFAKMVLKLSE"
     gene            2179..2373
                     /locus_tag="PK754_11545"
     CDS             2179..2373
                     /locus_tag="PK754_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10183.1"
                     /translation="MKWLTQSQKLYVLIFKISLFFVMPLLLLTVLPMIGVAWLSEIAL
                     AALVAGMILTITKLRERIFL"
     gene            2393..3295
                     /locus_tag="PK754_11550"
     CDS             2393..3295
                     /locus_tag="PK754_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10184.1"
                     /translation="MKNYFKTILLFLSFVLTYALQIKAQEPDSASPLKPLVEAERNFA
                     KMAAAEGVRNAFVHNLADKALMFRNGPINGKELWEKRKPAPFLLKWEPEFADIAASGD
                     FGFTTGPWEIQEYRPLTESGGYGYFTSVWKKQTDGTWKVVLDIGTSNPKPAAYTYALQ
                     YPPGADKVRSFTPVAKLDAVRDELIQRDAALGRSTAASFSAPSFFEVLSSDARLQRNE
                     YLPTTHRDTIAARLRGITAWESQPIGGDVAASGDLGYTYGTYAIRRGETSEKGYYVRF
                     WKRNEKNQWKITLDLTDPSPEVKK"
     gene            3849..>5944
                     /locus_tag="PK754_11555"
     CDS             3849..>5944
                     /locus_tag="PK754_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNB10185.1"
                     /translation="MKNEKLTRKEIIDNRLKQAGWNVTDRTQVVEEFDIIVDKNLVQE
                     ASTPYAGHQYSDYVLLGKDGKPLAVVEAKKTSVDAAIGREQAKQYCYNIKETQGGELP
                     FCFYTNGHDIFFWDLDNYPPKKVHGFPTRDDLERYLYIRKARKSLAGELINTKIAGRD
                     YQIRSIRAVMEAVEKRKTKFLLVMATGTGKTRTCIALVDALMRAGWAERILFLVDRVA
                     LQNQTLDAFKEFLPNEPRYPKPYEKEIATDRRIYVSTYPSMLNIIRDEKNPLSPHFFD
                     LIVVDESHRSIYNTYQEILDYFNTIPLGLTATPTDVIDHNTFQLFECEDGVPTFAYSY
                     EEAVNHIPPFLSNFQVMKIKTKFQNEGISKRTISLEDQQKLIVEGKEIEEINYEGTEI
                     EKKVINRGTNALIVREFMEESIKDTNGVLPGKTIFFCISKAHARRIEEIFDSLYPEYK
                     GELAKVIVSEDPRVHGKGGLLDQFTNNDMPRVGISVDMLDTGIDIREVTNLVFAKPVY
                     SYTKFWQMIGRGTRLLEQEKIKSWCTQKDNFLILDCWDNFEYFKLNPRGKELKGQIPL
                     PVRLFGVRLEKIEEAQTQSNSEIVNKEVQKIRRQAESLPKNSVVIMEAKHELQRLEDE
                     NFWSNLTSDKIEFLEAVVKPLLRTVSDVDFKAMRFEKDIVEVSLAHLAGETEKFEALK
                     ESIIEEIGELPLSINIV"
BASE COUNT         1850 a         1328 c         1252 g         1514 t
ORIGIN      
        1 agttatacaa agccttggat gacaaacctt ccgcccgtaa agccttaacc cgggctatcc
       61 aatgcccgat cagcgaagcg gaaaaacatt tattaaacga tcgtttagaa aaattaactt
      121 agtttaattt tagtaagcgc ttgcgatcaa cccactatat catttcgaaa gaaaaatatc
      181 tatgcgacat aacatcaaaa ttttaatcgc atcagttttg tttttagcat gcaacaatcc
      241 tgaacataaa atttcggcac aatcggagaa tgaaaaaatc gtccggcagt attatgaata
      301 ctttaataat cacgcatggg aaaaaatggc aggcatgtac acggaggtat cggaatttaa
      361 agatccttcg ctgggacccg gtatcgtaaa acaaacaagg caacaaatca ttcaaaaata
      421 caccgaattg agtacgatgt tccccgacgt caaagacgaa atcattcaga cctacccttc
      481 cggtgataaa catatcatcg ttgagtttgt gtctaccggg acggcgcccg ataacaccaa
      541 gcttcaatta ccgatttgta ccgtttttac gattgagaat ggcttgatca gcaaagactt
      601 cacgtattac gataattttg aaacaaaaac gacgaactga tttttctggt tcttagtact
      661 aatccggcat acgacgcagc tttacgcttc acgcatcacg catgccgatg aaacttttgt
      721 ttacgaattt acgtttgagg tggtaacact ttgtaacacg taaaatccga cgtgttgatg
      781 gcattctaaa acgaaatgaa ttatgaaaaa tgcgatgtac gggctgttat ggagttggat
      841 tctggtttcc gttgtgcacg ggcaaactat tgataaatac aaactggacg tgcttttaaa
      901 aaaagccgaa cagacgcatt cggaagccgt gatcgtctat aaggacaacc aactcgttac
      961 cgaaaaatat ttcggaatcg gacaggccga taccaaaatc gaaagcatgt cctgcaccaa
     1021 aagtattgtc ggtttggccg tggcgtgtat gctctccgat caaacgctgg ctagtctgga
     1081 tgaacccgtg tcccgatatt atccggaatg gcggcaaggc caaaaacaag cgatcacagt
     1141 gcggcattta gtcaatatga catccggttt gcaaaatcat cccaatgctt ccgttgaaat
     1201 ctatccgagc cccgacttca tacagcttgc gctggccgcg gaattaacat ccaaacccgg
     1261 cgaagtctgg tcctataata acaaatcact aaatcttatg gccggtgtga tcaaaaaaat
     1321 caccggccaa cgcatggatc agtatattgg cgaacgcctt ttcaaaccct tgggtatcac
     1381 ggatttcagt tggtcgctgg acagtgccgg taacccgcac gtcatgtccg ggtgccaaat
     1441 caaacccggc gacttcgcta aaatcggact tctcttgctc aataacggaa aatacaataa
     1501 ccaaaccgtc atcgctgaaa aatacataca agaagtgatc acaccgtgca aaccgtatcc
     1561 gggttacggg atactgtggt ggattgacta cgaaaaaacg acctccatcg tggatgatga
     1621 gatcgtgcgt gaacttcaga aagcaggcgt tgcagaagac tttatccaaa aagccaaggc
     1681 catgcaaggc acgtacgcaa ataacgatgc ctactatgcc aaactactca gcgttttcgg
     1741 agctaatcca tgggaataca tcaatacgac gctcggcgcc aatcttcgac tgcgcaaaaa
     1801 agaatttagc ggccatgtga cctatcgggc ggacgggtat ttaggaaact atatcatcgt
     1861 ggattttaaa accaaaatcg tcgccgtgcg tatgatcagc caccaaagtt ttcaggacga
     1921 aaccgataac ttcaatgatt ttgcaaaaat ggtattaaaa ctatccgaat aaaataatgc
     1981 gcttatcgga atgactcatt tacgtcatgg taagcggagt cgaaccatga ccaagtttgg
     2041 taaaatccgt cttcgacttc gctcagacag aagggcctct aaacccggtt tttctgaaac
     2101 tttttcctct gtatgcgtat atctctatta cgatcgcatc cgtagtctgt tctacaaagt
     2161 ttcaggaaag gctctatcgt gaagtggtta acacaatctc aaaaattgta tgtactcatt
     2221 ttcaaaatta gtttattttt cgtcatgcct ttgcttttac taacggtact gccgatgatc
     2281 ggtgttgcct ggttatccga aatagcctta gccgctttgg tcgccggtat gatcctgacg
     2341 atcaccaaac tgcgcgaacg tattttttta taaatctaac cccgtatgtc ccatgaaaaa
     2401 ttatttcaaa accatccttc tttttttgag ttttgttttg acctatgcgt tacaaatcaa
     2461 agcgcaggaa cccgactcag cctcgccgct caaacccctt gtcgaagccg agcgcaattt
     2521 tgccaaaatg gccgccgccg aaggcgtgcg caatgccttc gttcacaatc ttgccgataa
     2581 agcattgatg tttcgcaacg gccctatcaa cggcaaagag ttatgggaaa agcgcaaacc
     2641 cgcgccgttt ttactcaaat gggaaccgga gtttgccgac atcgccgcct ccggtgattt
     2701 tggttttacg acaggtcctt gggagataca ggaatatcgc ccgctcacgg agtccggcgg
     2761 ctacggttac ttcacatcgg tatggaaaaa acaaaccgac ggcacatgga aagtcgtact
     2821 cgacatcggc acatccaatc cgaaaccggc agcatacacc tacgctttgc agtacccgcc
     2881 gggcgccgat aaagtccgat cgtttacccc cgtcgctaaa cttgatgccg ttcgggacga
     2941 actgatccag cgcgacgccg cgctgggccg ctcgacggca gcgtcattct cggccccgtc
     3001 gtttttcgaa gtgctatcaa gcgatgcacg tttgcagcgc aatgaatacc tgcccacgac
     3061 ccatcgcgat acgatcgcag cacgtttgcg cgggatcacc gcttgggaat cccaacccat
     3121 cggcggcgat gtagccgctt ccggcgacct cggctacacg tacgggacgt acgccatccg
     3181 gcgcggtgag acttccgaaa agggctacta cgtgcgcttc tggaaacgta acgaaaaaaa
     3241 ccaatggaaa atcacactgg atctcaccga tccttcaccg gaagtcaaaa aataaaactg
     3301 tcgctgattt tttatcttgg cgtcttcgcg agaacgtatc ccacatcccg gtcattgcgt
     3361 aggtttgctg taccgaaatg accaggaaac caatccctcg caaagtcgct aggagaacct
     3421 gaaaaatatt cttaataaac ctcttgtacc ttggcgtctc tgcgagaacg tattctatcc
     3481 cggttactgt gtagacttgc cttatcgaaa tgaccaggaa acccaatcct cgctcagtca
     3541 catccgaaag aattgattct gtataatagt taaaatcatc atgcacttcc gtgtgtaacg
     3601 cctgctcgta tccatcgatt agtaacgtct cggcgagaat catttttttt caacacatcc
     3661 atcgtatgcc gtgaatgcga tattcccgca cagccttgta cgtattaact cttgcataaa
     3721 acctgtgata ggcgcatctt tatattaatc tctaaaactc caaaaaagat ttaatgtttt
     3781 tcgcatggca atgtacaacg aatataaatc actgcgttag aattaaacct attaattttc
     3841 atttttaagt gaaaaacgaa aaactgacaa ggaaagaaat cattgacaac cgtttgaaac
     3901 aagcgggatg gaatgtgact gaccgaactc aggtagttga ggagtttgac atcattgttg
     3961 acaagaacct tgttcaagag gcctcgacac catatgcagg gcaccaatat agcgactatg
     4021 tattattagg caaagacgga aaaccgcttg cagttgtaga agccaaaaag acttcggtag
     4081 acgctgcaat tggaagagaa caagcgaaac aatattgtta caatattaag gaaacacagg
     4141 gtggagaact tcccttctgt ttttacacca acgggcacga tattttcttt tgggatttag
     4201 ataactatcc gccaaagaaa gttcatggct tcccgacaag agacgacctt gaacgctacc
     4261 tatacatacg caaggcacga aagagtttag ctggtgaact catcaataca aaaattgcag
     4321 ggagggatta tcaaatccgt tccattcgtg ccgtgatgga agccgttgag aaacggaaaa
     4381 caaaattcct tctggtaatg gcaactggca cgggtaaaac acgaacctgt attgccttgg
     4441 tggatgcact aatgagagca ggctgggctg aacgtatttt atttttagtt gacagagttg
     4501 ctttgcaaaa tcaaacactt gatgcattca aagaattcct acccaacgaa ccacgatacc
     4561 ctaaaccata cgagaaagaa attgcaaccg acagacgaat ttatgtttca acatacccaa
     4621 gtatgttaaa tattattcgt gatgaaaaaa atcctttaag tcctcacttc tttgatttga
     4681 ttgttgtgga tgaaagccac cgcagtattt acaatactta tcaagaaatt ctagactatt
     4741 tcaacacaat tccattgggt ttgacagcaa caccaactga tgtaattgac cacaacactt
     4801 ttcagctatt tgagtgcgaa gatggcgttc caacttttgc atattcctac gaagaagcgg
     4861 taaatcacat tccgccattc ctgagtaatt ttcaggtaat gaaaattaaa acgaagtttc
     4921 agaatgaagg aataagcaag cgaacaattt ctttggaaga ccaacagaaa ctaattgttg
     4981 aaggaaaaga aattgaagaa ataaactacg agggaacaga gattgaaaag aaagttatca
     5041 atcgaggcac aaacgctttg attgttcggg agttcatgga ggaatcaatc aaagatacaa
     5101 acggggtatt acccggaaag acaatcttct tttgtatcag caaagcacat gcaagacgaa
     5161 tagaggaaat ctttgattca ctttaccccg aatacaaagg cgaattagcg aaagttattg
     5221 taagtgaaga cccaagagtt cacggcaaag gcggattact tgaccagttc acaaataatg
     5281 acatgccacg tgtaggtata agcgtcgaca tgcttgacac aggcattgac atccgtgaag
     5341 taaccaatct tgtttttgca aaaccagttt acagttacac caagttttgg caaatgattg
     5401 gacgtggcac acgattattg gaacaagaaa aaataaaatc ttggtgcaca caaaaagata
     5461 attttctgat tcttgattgt tgggacaact ttgaatattt caaactcaat ccgagaggta
     5521 aagaactgaa aggtcaaatc cctttgcctg ttcgcttgtt tggtgtgcga ttggaaaaga
     5581 ttgaagaagc ccaaactcaa agcaattccg aaattgtaaa taaagaagtt cagaaaatca
     5641 gaaggcaggc cgaatcactg cctaaaaact cggtagtgat tatggaagcc aaacacgaac
     5701 tgcaacgctt agaagatgaa aacttctgga gcaacctgac ttctgataaa atagaatttt
     5761 tagaagccgt tgtaaaacca ttgcttcgca cggtttcaga tgttgatttc aaagccatgc
     5821 gttttgaaaa ggacattgtg gaagtttcat tggctcattt ggctggtgaa actgaaaaat
     5881 ttgaagcgct gaaagaaagc atcattgaag aaattggcga actgcctttg agcatcaaca
     5941 ttgt
//
LOCUS       DAOQNQ010000203         5941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_5266_length_5941_cov_5.920319, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000203 DAOQNQ010000000
VERSION     DAOQNQ010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5266_length_5941_cov_5.920319"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="PK754_11560"
     CDS             complement(<1..1318)
                     /locus_tag="PK754_11560"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNB10186.1"
                     /translation="MRSLITIFLTISCTLYAQVLDNDETVTRADSLRGMLTPLRTCFD
                     VTHYALDVKIDTTTRRISGSNTITLVAMQDFTRLQIDLFDHYAIDRIEWKGKKLKFSR
                     EFNAVFVEFPETIRKSSRQSFTVFYSGTPIVAKNPPWDGGFIWSRDKSGNPWIAVACQ
                     GFGASSWWPNKDHQSDEPDSMLISITVPKGLTNVSNGRLRKQKDVGSGWTRFDYAVTY
                     PINNYNVTFNVGKFAYFGETYTSYDGQKLTCDYYVMPYNLEKAKVQFAQVKGMMDAFE
                     KSFGKYAFYRDGYKLVESPHLGMEHQSAVAYGNNYVNGYKGTGQAAEGLLFDFIIIHE
                     TAHEWWGNAVGSNDLADMWIHESFGNYAETIYLEGVYDYPTSLRYINANKTLVKNDRP
                     IIGPRGVNKEGSGDMYPKGGLMLNTLRSVINNDKLWFEILRGIQDTY"
     gene            complement(1360..2775)
                     /locus_tag="PK754_11565"
     CDS             complement(1360..2775)
                     /locus_tag="PK754_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNB10187.1"
                     /translation="MSNATGQKFSIKTFFSHILDGIRGTEHDYTEGSLGKAITLLSIP
                     MVLEMFMESLFGIVDVYFVSRLGPDAVASVGLTESMLTIIFAVAIGLSMATTAMVARR
                     IGEHNPEAASIAGFQAVLIGVAVSLPVSVIGLTMTPWLLEQMGASAGIIENGTGYAVW
                     MIGGNLTIVLLFLNNAIFRGAGDAVTAMHALWVANLLNMVLDPIFIFGFGPVPAMGVT
                     GAAIATNIGRGVGVLFQLYILIYGKRRLVFSPAVMRLDPEVLWNLIKVSVGGIFQFFI
                     TTASWLGLVRIVAMSGSAALAGYTIAIRVIIFAIMPSWGMSNAAATLVGQNLGAQKPD
                     RAERAVWFTARANMFFLGVVAILFISFAEMIIAWFTDDSQVVYYAVDCLRIISYGYVV
                     YACGMVMVQAFNGAGDTRTPTWINLGCYWMFQIPLAYTLAVIYGFGAQGVFAAIPIAE
                     SVATVVSVIMFRKGHWKLQKI"
     gene            complement(3321..5801)
                     /locus_tag="PK754_11570"
     CDS             complement(3321..5801)
                     /locus_tag="PK754_11570"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010855346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNB10188.1"
                     /translation="MFDLDMIKRVYAGMKAKQDRARKLFGRPLTLTEKILVSHFAENP
                     KAAPARFKDYVDLAPDRVAMQDATAQMALLQFMSSGKAKVAVPSTVHCDHLIQAEVGA
                     ADDLKKANSDNKEVYDFLASVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSH
                     TPNAGGLGMIAIGVGGADAVDVMAGLPWELKWPGITGVKLTGSLSGWTSPKDVILKLA
                     GILTVKGGTGKIVEYFGPGTASISCTGKGTICNMGAEIGATTSIFPYDMRMAAYLRTT
                     ERAAVAKLADQYAALLTADEGSEAYYDEVIEINLSELEPHVNGPFTPDKAWKISELKQ
                     AVADNNYPDEIRVALIGSCTNSSYEDIERSASLARIASQHGLKAKAEFVITPGSEQIR
                     ATIERDGQLEDFAKIGGKVLANACGPCIGQWKRHGVSEGDPNTIVTSYNRNFKARNDG
                     NSGTHAFVASPEIVTALAIAGRLSFNPMTDTITNAEGQQIRLLPPDGDELPRNGFTPG
                     ESGYVTPAKDGSKVQVIIDPASSRLQALSPFTAWDGRDIENAVVLLKAKGKCTTDHIS
                     PAGPWLKFRGHLDNISNNMFIGAVNAFTGEAGKGKNVITGETKGYSDNARYYKSQSIS
                     WITIGDENYGEGSSREHAAMEPRFLGGRAIIVKSFARIHETNLKKQGMLALTFKNPAD
                     YDKIREDDRLAIRGLTAFTPGVPLTMDIKHNDGSVETIELNHSYNAQQIGWFKAGSAL
                     NLIGGKTAASSRKKTATKTKKAAKKKTVIKEAKAKTKTKKSAKVAPKKQAAKSAAKKK
                     TKKAVKKTIKKATKKTNRKPVKKTSRKR"
BASE COUNT         1563 a         1467 c         1341 g         1570 t
ORIGIN      
        1 ggtatgtatc ctgaataccg cgtaatattt caaaccaaag tttatcatta ttgatcacac
       61 tacgcagtgt attgagcatc aatccgcctt taggatacat atcgccggag ccttctttat
      121 tcacgccccg cgggccgatg atcggacggt catttttgac gagcgtttta ttggcattga
      181 tatagcgcag agacgtcgga taatcataca caccttcgag ataaatcgtt tcggcgtaat
      241 ttccgaaact ttcgtgaatc cacatatcgg ccagatcatt ggaacctacg gcgttgcccc
      301 accattcgtg tgctgtttca tggatgatga taaagtcaaa caacaatcct tccgccgctt
      361 gccctgtacc tttgtaaccg ttgacgtaat tattaccata cgctacggcg ctttgatgct
      421 ccatacccaa atgcggtgac tcgactaatt tatacccgtc gcgataaaac gcatatttgc
      481 cgaacgattt ttcaaacgcg tccatcatac ctttgacttg agcaaactgt actttggctt
      541 tttcgagatt gtacggcatg acataataat cgcatgtcaa cttctggccg tcgtaggatg
      601 tataagtttc tccaaaatac gcaaatttgc ccacattgaa tgtgacgtta taattattga
      661 tcggatatgt aacggcataa tcaaaacgcg tccaaccgga gcccacatct ttttgttttc
      721 gcaagcgacc attggaaaca ttggtaaggc ctttgggtac ggtaatgctg atgagcatac
      781 tgtcgggttc gtccgactga tgatctttat taggccacca actgctggcg ccgaaaccct
      841 ggcatgcgac agcaatccac ggatttcccg atttgtcacg cgaccaaata aatccgccgt
      901 cccagggcgg atttttagcg acaatgggtg tgcccgaata aaaaacggta aaactctgac
      961 gggagctttt gcgaatcgtc tcaggaaatt ctacgaacac ggcattgaac tcacgagaaa
     1021 acttaagttt cttacctttc cactcgatcc gatcaatcgc gtaatgatcg aataaatcaa
     1081 tttgtaaccg ggtaaagtcc tgcatcgcga ccaacgtgat cgtattgctt ccgctgatac
     1141 ggcgtgtagt ggtatctatt tttacatcca gagcataatg cgtcacatca aaacatgttc
     1201 gcagcggcgt cagcataccg cgcaaagaat ccgcacgggt tacggtttca tcgttatcta
     1261 aaacttgcgc gtacaatgta cagcttatcg taagaaaaat agttattaaa cttcgcataa
     1321 cgtttttcct ttggtatgtg atgagtgaca aaaaacaatt catatttttt gcagtttcca
     1381 atgtcctttt ctaaacataa tcacgctgac gacggttgca accgattccg cgatcggtat
     1441 agcggcaaac acaccctgag cgccaaaacc ataaatcacg gcaagcgtat atgccagagg
     1501 gatctggaac atccaatagc aaccgagatt gatccacgtc ggcgtgcgtg tatcgccggc
     1561 accgttaaat gcctgcacca tcaccatacc gcaggcatac acgacataac cgtagctaat
     1621 gatacgtaaa caatccacgg cataataaac gacctgtgaa tcatcggtaa accaagcgat
     1681 gatcatctca gcaaatgaaa taaaaagaat tgccacaacc ccgagaaaga acatattcgc
     1741 acgcgccgtg aaccacaccg cgcgttcggc acgatcgggc ttttgagcgc cgagattttg
     1801 accgaccagc gtcgccgccg cgttactcat accccaggac ggcatgatgg caaaaataat
     1861 cacgcgtatg gcgatagtat aaccggccaa agccgcgctg cctgacatcg cgacgatgcg
     1921 tacgagccca agccagcttg ccgtcgtaat aaaaaattgg aaaatgcctc cgacagatac
     1981 tttgatcagg ttccacaaaa cttcgggatc caatcgcatc acggcgggcg aaaaaacaag
     2041 ccgccgtttt ccatagatca gaatgtataa ctgaaacaac acacccacgc cgcgaccgat
     2101 attggttgct atggccgcgc cggtaacacc catggccggc acgggaccaa aaccgaaaat
     2161 aaaaatcgga tcaagaacca tgttgagcaa attggcaacc cacaaagcat gcatggctgt
     2221 gacagcatcg cccgcgccgc gaaaaatagc attattcaga aaaagtaaaa cgatggtgag
     2281 attgccgccg atcatccata cggcataacc ggttccattt tcgataatac cggccgaagc
     2341 gcccatttgc tccaaaagcc aaggcgtcat cgtcagtccg atcacgctga ccggtaagga
     2401 aacagccacg ccgatcagta cggcctgaaa acccgctatg gaagccgctt cggggttatg
     2461 ttcgccgata cgccgagcga ccatagctgt cgtcgccata ctaaggccaa tcgccacggc
     2521 aaaaataatc gtcaacatgg attcggttaa cccgacggaa gctaccgcat cgggaccaag
     2581 ccggctgaca aaatacacgt ccacaatacc gaacagcgat tccataaaca tttccaaaac
     2641 catcggtatg gataaaagtg taatcgcttt tccgaggctg ccttccgtat aatcgtgttc
     2701 ggtgccacga atgccgtcga gaatatggct gaaaaacgtt ttaatcgaaa atttctgacc
     2761 tgttgcatta gacatgatta ccgtcttgat aaataatgaa atggatttct gaattggtaa
     2821 aacaaaagac aggttacgct ttgtacgaaa agtttaacct gatgttgcgt agaaagcgct
     2881 tgtttttcat ttattcatac ataaaacgcg tagtgtgaat taggcaataa catatttgaa
     2941 tttaattttt tatggttttg taggtttacc agctgaaaaa gagagattaa gtatctcata
     3001 attgtatgga cacccgcatg cgcgggtgag acaatctatc cactactctg tcgttcccgc
     3061 gaaagcggga accttcattg attcatcgtc gtcttgcctt tagtcgggca acacaaaggt
     3121 ttgattgaaa caaacgtgca ccattaaaat ttgcggaaat tcaataatga gactattaat
     3181 tcacacaaca atctaaataa aataaaaaag gctgcatcat aagctacgac atatcgctgt
     3241 gtatttttcg gcatgatgta caaaccgctg cgatcgtatt cgtaaacttt tgatacagcc
     3301 cggtaaggga aatgaaaagt ctatcgcttc cgtgaggttt ttttcacggg tttacggttt
     3361 gttttctttg tcgctttttt gattgtcttt ttaacggctt tttttgtttt tttcttagct
     3421 gctgacttag cggcttgttt tttgggagcg actttggcgc tttttttggt tttggtcttt
     3481 gctttcgctt ctttaattac cgtttttttc ttggctgctt ttttggtttt agttgccgtt
     3541 tttttacgcg acgaagcagc cgttttgccg cctatcagat tgagtgcgct accggctttg
     3601 aaccaaccga tctgttgcgc gttatacgaa tggttgagtt cgatcgtctc cacggaaccg
     3661 tcgttatgtt taatgtccat cgtcagcggc acacccggtg taaacgccgt caacccgcgg
     3721 attgccagac gatcgtcttc acgaatttta tcatagtccg ccgggttttt aaatgtcaac
     3781 gccaacatac cttgtttttt gagattcgtt tcgtgaatac gtgcaaacga tttgacgatg
     3841 atggcgcgac cacccaagaa acgcggttcc atcgccgcat gttcgcggct tgatccttca
     3901 ccgtaatttt cgtcgccgat agttatccaa ctaatgctct gggatttata ataccgtgca
     3961 ttatcggaat agcctttggt ttcgccggta atcacatttt tacctttgcc ggcttcaccg
     4021 gtaaatgcat taacggcgcc gataaacatg ttgttggaaa tattatccaa gtgcccgcgg
     4081 aatttgagcc acggtccagc gggagaaata tgatccgtcg tacatttgcc tttggctttg
     4141 aggagtacca cggcattttc gatatcgcgg ccatcccatg ccgtaaaagg actcaatgcc
     4201 tgcaaacgcg aactcgcggg atcaatgatc acctgcactt tggaaccgtc ttttgccggt
     4261 gtgacataac cactttcgcc gggcgtaaat ccgttgcgcg gcaattcatc accgtcgggc
     4321 ggcaataaac gaatttgttg accctccgca ttggtgatcg tgtccgtcat cggattaaag
     4381 ctcagacgtc ctgcgatggc cagtgccgtt actatttccg gactggcaac aaacgcatgg
     4441 gtaccggaat taccgtcatt gcgggctttg aaattacgat tgtacgatgt gacgatggta
     4501 ttcggatcgc cctcgctgac gccatgacgt ttccactgac cgatgcacgg accgcaggca
     4561 ttggctaaaa ctttaccgcc gattttagcg aaatcttcca attgaccgtc gcgttcaatc
     4621 gtcgcacgaa tttgttcgga gccgggcgta atcacaaatt cagctttggc tttaagaccg
     4681 tgttgcgaag cgatgcgcgc taaactggcc gaacgttcga tatcttcgta cgatgaattg
     4741 gtacacgaac cgatcaatgc cacacggatt tcatccgggt aattattatc ggcaacggct
     4801 tgtttcaatt cagaaatttt ccatgctttg tccggtgtga aaggcccgtt gacatgcggc
     4861 tccaattcgc tcagattgat ttcgatgact tcatcgtaat acgcttccga cccttcgtcg
     4921 gcggtaagca acgcggcgta ctgatccgcc agtttggcca cggcagcgcg ctccgtcgta
     4981 cgaagatacg ccgccatacg catgtcatag ggaaaaatag aagtggtcgc gccgatctcc
     5041 gcacccatat tacaaatcgt tcctttaccg gtacaggaaa tggatgccgt gccgggacca
     5101 aaatattcga cgattttccc ggtgccgcct ttgactgtca gaataccggc taatttgaga
     5161 ataacgtctt tgggtgaagt ccatccgctg agcgaacccg tgagtttgac accggtaatg
     5221 ccgggccact tgagttccca cggcaatccc gccatgacat ccacggcatc cgcgccgccg
     5281 acaccgatgg cgatcatgcc aaggccgccg gcattgggtg tgtgcgaatc cgtgccgatc
     5341 atcataccgc cgggaaacgc ataattttca agcacgactt gatggataat acctgcgccg
     5401 ggtttccaaa agccgatacc gtatttattg gagacgctgg ccaaaaaatc atacacttct
     5461 ttgttatcac tattggcttt tttcaaatcg tccgccgcac cgacttcggc ttgtatcagg
     5521 tgatcgcagt gcaccgtaga aggcacagcc actttggctt tgccggaaga cataaactga
     5581 agcaacgcca tttgtgcggt cgcatcctgc atggctacac gatcgggtgc aagatctacg
     5641 taatctttga accgtgcggg cgcggctttg gggttttccg caaaatggga aacgagaatt
     5701 ttttccgtca gcgtgagcgg acgtccgaaa agtttgcgtg cgcggtcttg tttggccttc
     5761 ataccggcat atacgcgctt tatcatatcg agatcaaaca tggcagtaaa actccttgat
     5821 cataatttta tgtacataaa agtagaaaga ttgttctgaa ttggctgatg cattattagt
     5881 cggtgagtta ttcatgcgag cgcaaaatac ataacttaga aagtttacgc aagggaatat
     5941 c
//
LOCUS       DAOQNQ010000204         5900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_5328_length_5900_cov_5.579127, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000204 DAOQNQ010000000
VERSION     DAOQNQ010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5328_length_5900_cov_5.579127"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1632)
                     /locus_tag="PK754_11575"
     CDS             complement(<1..1632)
                     /locus_tag="PK754_11575"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNB10189.1"
                     /translation="MSGDGLTTNLNDAQKIAVQTTDGPVLILAGAGSGKTSVLTRRIA
                     YLIAEKQISPFNILAVTFTNKAAGEMKHRIERLVGYHSQKIWIGTFHSIFGKILRNEA
                     EKIGYSKNYTIYDSDDQLNLVKISMEYLGISQELYSPNIIRAKINKAKNTFQNPSQFH
                     KSVDNPMDEKAALVYTDYEKRLRQASAFDFDDLIVKPIELFQQFPETLTFYQEKFHYI
                     HVDEYQDTNRAQYILMKMLAAKYRNLCVVGDDDQSIYGWRHADIGNILNFEKDYPEAS
                     VYKLEQNYRSTANILQVANEVVRNNEIRKDKSLWTDKAQGKLITVFECDDDRAEAKNI
                     AQVIQKKIQQEKIRFADFAVLYRTNAQSRVLEDALRQMSIAYVIVGGVRFYDRKEVKD
                     VMAYLKLISNPADVISLQRVINFPARGIGKTTLEKLEEFSQVKNMPLFDAFRNVRSFG
                     AGLIQERTMAAIVSFCEFIQKYQELRTQMGFGEWVRILIDELGLLKMYQQENSVEAIS
                     RYENIVELLNAVSEFSMKYDAEQAEEPVLEAFLKEV"
     gene            complement(1753..5841)
                     /locus_tag="PK754_11580"
     CDS             complement(1753..5841)
                     /locus_tag="PK754_11580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNB10190.1"
                     /translation="MTFVVIDSSDGGQLTSTIPVLTDINGQAIAVYQTGTNVGYNRVA
                     AISYIPGSVIGMPDSTYFELITIPLDANAQPVASSLSPTIVSRNQNVSFKYSFVNTGT
                     FPVNLIGDSTYIQITHTGSGSSYRTTLDTNVNKTIGITGATQLTFRADTVKLPSGVYG
                     TGGDSMRIVLYGTLFDSAAATLDTLKFTFFTDQLDPLTVQNPAFLVIDSVLIDSALAV
                     QGQDSILVRYYVRNTGEADAISFTVTDSISNAGATVTNDWVLLATNKPSTISGLSSQN
                     FTQYYRVKGNAVLGTDRISSMVRAVDYNDAAQIKYDTLYTSDTLGIRVPAQLQITRTY
                     IDTVYNTGILNRDQIVRIKSLVRNFGEESANLRVHFESDAGNADLDSMLTTTLNGGDS
                     LWAVSRYFRTRNASGAESYRAFVDSGYGLISGRPLDIVDPISGNQVTFTLQDSAELKL
                     NLFVDIPGDSLEVSDTANFNLRVTVTNIGEASLNTALVPVRVRLPINNMFRYQASPTL
                     DSVYFVTVGSTFTIPMSSYQETPDYDSVVALLDTANAAFPLDVNTNSKSAIHTVRDSV
                     FLKTSNTGNLIATRIRILSPNGAVDGTVSTFQGFTVETVVKDLSRLSGMQARMILPSE
                     GLSTRDSLAQAFIQNVNDSVVTTWRIFSNTDNDSVYPLRVVYSGIDSTTGDTVISDTL
                     KLSVTVVRRAEMTTTLRITGPVGALDDTLSFGQELSVEATIRNLGQAAFSGAGQITLN
                     VPVGFDVVAAPNVYTFTRDTMINWTLRANASGSLQMTAVVNVLPNDENTDQPVVVFSN
                     ADTQNVVVVNPAEILNASITIQGVPGDSVSTDQIFVVSAQFTQSRSLTARYATIQFPN
                     NSAYRLKDGEVVLKQISDLDNIVRWSVVAPSTLIIPTDNEQIDLARITFVGVDRNNKD
                     SNVTAQVDKNFTLQRKAVLRLRAAIIEPVDAIDNFVTKNQTFRIQATVSNLGYALPRG
                     QGMLLLDSLDAGNGLIFTEQDVANRDNIFDFDNGDFTASWLVRAPDGVVNHPLVLRFV
                     HLPTDTNTRDTVTVESRSFRLAVSTEAKQLRVERIATDPGTTIYVNGVVDSIMALRLK
                     NNSSTTNTNGMIVQEFKFVVETFDYKTRTYNDTNWTAYFDSITVRLMGEEVSFGRANN
                     LGTDEDTAVVRVDQNAGLKKLARALAVDLDYGLAILPGVAEEVVFKARVKPAVAGKNP
                     NFRLILVGVTAYDNDVLGSQDAGITDLPLLPAVDATGNLLGSPEANAGNTTQQMGIQD
                     PENGGKVAQFGNFPNPFGSSGKEKTTFRFRGTGEKAVIEIYTLAGNLVTKLTQNTVDD
                     TQVEMVWDGRNGNGEKVRNGVYIAVLKSGGEGKYTTKVMVVR"
BASE COUNT         1587 a         1384 c         1278 g         1651 t
ORIGIN      
        1 cacttctttc aaaaaggctt caagtaccgg ttcttcagct tgttcggcat cgtatttcat
       61 agaaaactca gataccgcat tgagtaattc gacaatattt tcgtaacgcg aaatcgcttc
      121 aactgaattt tcctgttgat acattttaag taacccgagt tcgtctatta atatccgtac
      181 ccattcgcca aaacccatct gcgtgcgtaa ctcctgatac ttttgaataa attcgcaaaa
      241 tgacacgatc gccgccatcg tgcgttcctg aatcaacccc gcaccgaaag agcgcacatt
      301 gcgaaacgca tcaaagagcg gcatgttttt cacctgagaa aactcctcaa gtttttccaa
      361 tgtcgtttta ccgataccgc gtgctggaaa attgatcaca cgttgaagac tgatgacatc
      421 ggccggattg gagatcagct tgagatacgc catcacatct tttacttctt tgcgatcata
      481 aaaacgaact ccgccgacga tgacatacgc gatagacatt tgacgtaacg catcttcgag
      541 tacgcggctt tgtgcattcg tgcgataaag aacggcaaaa tcggcaaaac gaattttttc
      601 ttgttgaatt ttcttttgga tcacttgagc gatgttctta gcttcggcac gatcatcatc
      661 gcattcaaac accgtgatca gcttaccctg tgctttatct gtccacagcg atttatcttt
      721 gcgtatttca ttgttacgaa caacttcgtt agcgacttga agaatattcg ctgtagagcg
      781 ataattttgc tcaagtttgt aaacgctggc ttcagggtaa tctttttcaa aatttaaaat
      841 attaccgatg tccgcatgac gccatccata gatagactga tcatcatctc ctacaacaca
      901 cagattgcga tacttagcgg caagcatctt cataagaata tactgtgccc ggttggtatc
      961 ttgatattca tctacatgga tgtagtgaaa tttttcctga tagaaagtca gcgtttcggg
     1021 aaattgctgg aaaagttcaa tcggttttac gatcaaatca tcaaagtcaa aggcgctggc
     1081 ttgccgtaat cgtttttcat aatccgtata aactagcgcc gctttttcat ccatcggatt
     1141 atcaaccgac ttatgaaatt gcgatggatt ttgaaatgta tttttggctt tattgatttt
     1201 ggcgcggata atattgggcg aatataattc ctgagaaatg cccaaatact ccatcgaaat
     1261 tttgactaaa ttgagctgat catccgaatc gtaaatcgta tagtttttag aataaccgat
     1321 tttttcagct tcattccgaa ggattttccc gaaaatagaa tgaaaggtgc cgatccatat
     1381 tttttgacta tgatacccga ctaatctttc gatacggtgt ttcatttcac cggcggcttt
     1441 attggtgaaa gttacagcta gaatattaaa cgggctgatt tgtttttcgg ctataaggta
     1501 agcaatacgc cgcgtaagaa cgctcgtttt accgctgcct gcgccggcta aaatcaaaac
     1561 cggcccatcg gttgtttgga ctgctatttt ttgagcatcg ttgaggtttg ttgtcaaacc
     1621 gtcgccggac atattttttc cttatttatt ttttggcgcg tgcaatgtat agaacgagct
     1681 attgaaatgc aaggtagcaa aaaatcctga ccggtagtga tcaggatttt ttctgaacaa
     1741 tttattttga gtttaacgta cgaccatcac ttttgtcgta tattttcctt cgccgccgga
     1801 tttcagtacg gcaatgtaaa caccgttacg cactttttca ccgtttccgt tacggccatc
     1861 ccaaaccatt tcgacttgtg tatcgtctac agtattttgt gtcaatttgg ttacgagatt
     1921 accggccaac gtataaattt cgataaccgc tttttcgccg gttcctctga aacggaaagt
     1981 cgtcttttct ttgccgctac tgccaaaagg attggggaaa ttaccgaatt gtgcaacctt
     2041 gccgccattc tccggatcct gtatgcccat ttgttgcgtt gtattacccg cattggcttc
     2101 cggtgaaccc aaaagattgc ccgtagcatc tacggcagga agcagcggaa gatccgtgat
     2161 gccggcatct tgcgagccca gtacatcatt gtcatatgct gtcacgccca ccaatatcaa
     2221 tctgaaattt gggtttttgc ctgcaacagc cggttttacc ctcgctttga atacaacttc
     2281 ttcggcgact ccgggaagaa tggccaaccc gtaatctaaa tccacagcca acgcgcgggc
     2341 tagtttcttc aatcccgcat tttggtccac acggacgacc gccgtatctt catcggtacc
     2401 tagattgttt gcacgaccga aactaacctc ttcacccatg agccgcaccg tgatcgaatc
     2461 gaaataagcg gtccagttgg tgtcgttata cgtgcgcgtt ttgtaatcaa aagtttcaac
     2521 tacaaattta aattcctgaa cgatcattcc gttggtgttg gtcgtggaag aattattctt
     2581 caaccgcaat gccatgatgc tgtcaacaac accgtttacg taaatcgttg tacccggatc
     2641 cgttgcgata cgttccactc gaagttgttt agcttcggta gaaacggcaa ggcggaatga
     2701 acgcgattca accgtcacgg tatcgcgtgt attcgtatcc gttggtaaat ggacaaagcg
     2761 cagtaccaaa ggatgattaa ctacaccatc cggagcacga acaagccacg atgcggtaaa
     2821 atcaccattg tcaaaatcaa atatgttgtc tcggtttgcc acatcctgct ctgtaaagat
     2881 caaaccattg cccgcatcta acgaatccaa aagcaacata ccttggccgc gcggtaacgc
     2941 ataaccgaga ttggataccg ttgcctgtat gcggaatgtt tgattttttg tgacaaaatt
     3001 gtctatagca tctaccggct cgatgatcgc agcacgtaaa cgcagaacgg ctttacgttg
     3061 caaagtaaag ttcttatcaa cctgcgccgt cacattgctg tctttgttgt tacgatcaac
     3121 gccgacaaat gtaatccggg ccaagtcgat ctgttcgtta tccgtcggaa tgataagcgt
     3181 tgaaggcgcc acaacactcc agcggacaat attatccaga tcggatattt gtttaagtac
     3241 tacttcacca tctttcagac gatacgcgga gttattcgga aactgtatcg tagcgtaacg
     3301 cgccgtgagc gaacgcgact gagtaaattg tgcgctgacg acaaatatct gatctgtcga
     3361 aaccgaatca ccaggaacac cttgtatcgt tatggacgcg ttaaggattt cagccggatt
     3421 gacaaccacg acattttgtg tgtccgcatt gctgaaaaca actacgggtt gatctgtatt
     3481 ttcatcgttg ggtaatacat ttaccacggc tgtcatttgt aatgaaccgg atgcgttagc
     3541 acgtaatgtc cagttgatca tcgtatcgcg tgtaaaagta tatacattag gagccgcaac
     3601 tacatcaaat ccgactggaa cattaagcgt aatctgaccg gcaccggaga atgccgcttg
     3661 acctaaatta cggatcgtcg cttcgacgct aagttcctga ccgaaggaca gtgtatcatc
     3721 caatgcacct acaggcccgg tgatacgcag tgtcgttgtc atttctgcac ggcgtacaac
     3781 cgtcacagac agtttgagtg tgtcgcttat gaccgtgtca ccggttgtag aatctatacc
     3841 tgaatacaca acccgtaacg gataaacgct gtcgttatcc gtattggaga atatccgcca
     3901 tgtcgtaaca accgaatcat ttacattttg tataaatgcc tgcgctaaac tatcacgggt
     3961 agacagacct tcacttggta aaatcatccg cgcttgcata cccgagagac gggatagatc
     4021 ttttactacc gtttcgactg taaatccttg gaatgtcgaa accgtcccat caacggcgcc
     4081 gttcgggctt aggatacgaa tgcgcgtagc aatcagattt ccggtattgc tggttttcag
     4141 gaaaaccgag tcccgcacag tatgaatagc ggatttacta ttggtattaa cgtctaatgg
     4201 aaatgcggca ttagctgtat ccaaaagtgc aactaccgag tcatagtccg gcgtttcttg
     4261 gtacgaactc atcggtattg taaatgtgct tccgaccgtc acaaaataaa ctgaatctaa
     4321 tgtgggtgaa gcctggtagc ggaacatatt gtttatcggt aatcggacac gcacaggaac
     4381 caatgccgta tttaacgaag cttcaccgat atttgtgacc gttacacgca gattaaaatt
     4441 cgccgtatcc gacacttcga gcgagtcacc gggtatatca acaaataaat tcaatttcag
     4501 ttcggccgaa tcctgcagag taaacgtcac ttgatttccg gaaatcggat caacaatgtc
     4561 taatggccta ccggaaatga gtccgtaacc agaatcaaca aaagcacgat aactttctgc
     4621 gccggatgca ttacgtgttc tgaaataacg cgacacggcc catagcgaat cccctccgtt
     4681 cagcgtcgtc gtcaacatac tgtcgagatc cgcattaccc gcgtcgcttt cgaaatgcac
     4741 gcggagatta gccgactctt caccaaaatt tctaaccagt gatttgatcc gaacaatctg
     4801 gtcacgattc aagatgcctg tattgtaaac cgtatcaata tacgtgcgcg tgatctgaag
     4861 ttgtgcaggt acgcgaatac ccaatgtatc agaggtgtac aaagtatcgt acttgatttg
     4921 cgcggcatca ttatagtcta cggctcgaac catactggat atgcgatccg tacccagcac
     4981 tgcattgcct ttcacacgat agtactgtgt gaagttttgt gaactcaacc ctgaaatggt
     5041 tgacggtttg ttggttgcca gaagcaccca atcgttagtt acagtagcgc cggcattgct
     5101 gatcgaatca gtaactgtaa aactgattgc atccgcctcg cccgtattgc gcacataata
     5161 acgaaccagt atgctatcct gaccttgcac cgccaaagct gaatcaatca ataccgagtc
     5221 gattacaagg aaagccggat tctgaacagt caatggatct agttggtccg taaagaatgt
     5281 aaacttcaag gtatccaatg ttgcggcggc cgagtcaaaa agtgttccgt agagtacaat
     5341 tcgcatgcta tcgccgcccg tcccgtaaac accggatggt aatttgaccg tatcggcgcg
     5401 gaacgtcaat tgggttgcgc cggtaatacc gatggtctta ttgacattgg tatccaatgt
     5461 ggtgcgatac gatgagccgc tgcccgtatg tgtaatctga atatatgtcg agtcaccgat
     5521 caggttgacg gggaatgtac ctgtattgac gaaactatat ttgaaggaaa cattttgatt
     5581 gcgcgaaaca attgtcgggc tcaacgatga agcaacaggt tgcgcgttgg cgtcgagcgg
     5641 tatcgtgata agttcaaaat aagtgctgtc cggcatgccg atcacgcttc ccggaatata
     5701 ggaaatagca gccacgcggt tgtatccgac gttcgttcct gtctgataca cagcaatcgc
     5761 ttgaccgttg atgtcggtaa gtaccggtat tgtactggtc aattgaccgc catcggatga
     5821 atcaataaca acgaaggtca ctaatgtatc cttcaccgga ttatcatata cgtcaagaag
     5881 ggttgccctc agcgtacggc
//
LOCUS       DAOQNQ010000205         5896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_5335_length_5896_cov_6.446670, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000205 DAOQNQ010000000
VERSION     DAOQNQ010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5335_length_5896_cov_6.446670"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..160
                     /locus_tag="PK754_11585"
     CDS             <1..160
                     /locus_tag="PK754_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019670496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB10191.1"
                     /translation="MAVLKKEGIQQIGDIEALEYGSFAWILDPNGIKIELWEPVKGYR
                     FKRKRSKK"
     gene            complement(189..1670)
                     /gene="guaB"
                     /locus_tag="PK754_11590"
     CDS             complement(189..1670)
                     /gene="guaB"
                     /locus_tag="PK754_11590"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNB10192.1"
                     /translation="MSLNFENKLVGEGLTFDDVLIVPMYSETLPNLVKVNTRLTKTIT
                     LNIPLISAAMDTVTESELAIAMAREGGIGIVHKNCSIEYQAAEVDRVKRSESGMIMKP
                     ITLTPEHKLSDALDLMKRYKISGIPITYADGKLAGILTNRDLRFATNYQNPIDTVMTK
                     NNLVTAKVGTTLEEAELILHKHRIEKLLVVDDSYILQGLITVKDIQKKKMYPHACKDK
                     HGRLRVGGALGVTKDTVERAQALLDAGVDVLGVDTAHGHSRGVLEMVKTLKQKFPQIE
                     IIAGNVATAEATEALIKAGADCVKVGIGPGSICTTRVVAGVGVPQITAILECARVAAK
                     AGIPIIADGGIKQTGDIAKAIVAGADTVMIGSLFAGTEESPGESVLYEGRQYKVYRGM
                     GSLSAMKQGSKDRYFQDDEMDTNKLVPEGIEGRVPYKGRLSDVVYQMVGGLRAAMGYC
                     GAESIETMKSKTRLMKMTGAGLKESHPHDIFVTKESPNYRMNS"
     gene            1850..5551
                     /locus_tag="PK754_11595"
     CDS             1850..5551
                     /locus_tag="PK754_11595"
                     /inference="COORDINATES: protein motif:HMM:NF015194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HNB10193.1"
                     /translation="MASKISTIRVGRILAVEREEYFKTGILFAYLFFMTAATIVGRTA
                     ADTLFLSRFDATKLSYMYLAISIALSICGVAFHAIVDRFRRDRLIYAVTGGMILAVGV
                     TRIFVDSGWSLMYPMIYVGFEVFNFLLILQFWTFTNDVLDTRQAKRLIGIILSAGILG
                     GMISGFSIKALVHAIGTSNLITMYGGLMGLCVILVRLLARREFTDTVRETHSVTDKKA
                     HKKAEEKHKLGSIYHLKLIAIIVSIITMVLMFIDYQFKMVLKEYYRNDELAAVLGQFY
                     GYTGIVALVFQFFLSSRILSKYGILVSLLILPIFLLISSLSIWFAPAIPGTVMIGSLA
                     LYLMPILNAVVFAKASDKIFSDTIYSSASQLMYVPLHANVRGRAKVFVDGIIKPLSKS
                     GSAILLILSAHFFPVAVNDISLAVVGLLIICIIAIFLVKREYVKSLFSTLKANQIDFK
                     DINFNVSDSSAIEILVQGLESQDDRRVLYCLDALKNIEGFYLIPHVLTLMHHKSPRVR
                     TEALRVIEKITPRQATGEVIKRLEDDHTEVKVQAILTLSAYADDNHIKRILSFLIDED
                     SEIKGAAISGLMQYYGIDGIFYSVDIFKQMLESPRINDRKEIARIIGRIGLKNFHKPL
                     IKLLDDPSKEVQIFAIDAAHRINAIDLIPMIIKKLKQKETRHKAMEALSAFDAADIVP
                     TLRTYLEFSRQYQEISLYIPRVFEMIASQECADIMLKNYDHAGPYLRLKILDSLAKIQ
                     FRNHDVELDRDLIEKWINLEFQEHRFFTQTLNAAKPFDGIAILIESVTHIRHGVFERV
                     FKLLGFLHDHKVITTVFLNLSNTDKRVQANAVEVLDNMLEGELRTSVVQIVNSHISHK
                     FNEERDAIKFFDTLMAAYGKDHDWLKMCVEFVSTSPKFDEWDHNRAFALRKLIHDTQV
                     SFERKEVLNIIQKVAILKRVSFFASLSGEYLANIALQMKSAKCHAGEAVFHEGETGDS
                     FYVVIKGKVLIHKGETRLVEMGPGQCFGEMAILDNEKRSASVTALEDTLMFKLESEAF
                     YDIIADRIEIAKGVIRMLTQRLRQQLAKVAAAGGGAPKPVIPQIEESAVEEMIEGESA
                     NIASELDSQALLKRVLLLKEIDLFASLPDEDMVMLANTIEEVKYKKGQDIFLEGDQGD
                     AMYGIVSGSVLIHKGNKDLATLGEKKYFGEMAVLDNEPRSASVTAVADTTLLKLSSDD
                     FYSILFDKVEITRNIFAVLVQRLRTTNTK"
     gene            5580..>5896
                     /locus_tag="PK754_11600"
     CDS             5580..>5896
                     /locus_tag="PK754_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNB10194.1"
                     /translation="MKICCISSREEARTAIQTGADALGLVSSMPSGPGVISDEIIRDI
                     AQIIPPPIASFLLTSKQDTAAIVTQQKFCGTNTIQICDHLTDGSYEDIRKALPGIAIV
                     QVV"
BASE COUNT         1477 a         1377 c         1424 g         1618 t
ORIGIN      
        1 gatggcggta ttaaaaaagg aaggtattca acaaatcgga gatattgagg cgttagaata
       61 cggttctttt gcgtggatac tggatcctaa cggtataaaa atcgaattat gggaaccggt
      121 caaaggttat cgttttaaac gtaaacgttc aaaaaaataa tcgaacgaac attatcggtt
      181 taaaaaaatc agctgttcat ccgataattc ggtgattctt tcgtcacaaa aatatcgtgc
      241 ggatggcttt ctttgagtcc ggcaccggtc attttcatca accgggtttt gcttttcatc
      301 gtttcgatgg attccgcgcc gcagtatccc atagccgctc ttaagccgcc gaccatttgg
      361 tacaccacat cgctcaagcg gcctttatac ggcacgcgcc cttcgatacc ttccggtacg
      421 agtttattgg tatccatttc gtcatcctga aaataacgat ctttgctgcc ttgcttcatc
      481 gccgaaagcg aacccatacc gcgataaacc ttatattgac ggccttcata caaaacggat
      541 tcacccgggc tttcttccgt gcctgcgaaa agacttccga tcatgaccgt atcggcgccg
      601 gccacgatcg ctttagcgat atcgccggtt tgtttgatgc cgccatcggc gatgatgggg
      661 attcccgctt tggcggcgac gcgtgcacat tcgaggatag ccgtgatttg cggtacgccg
      721 acaccggcga cgacgcgcgt ggtacagatg gaaccgggac cgatgccgac tttgacacaa
      781 tccgcgccgg ctttgatcaa agcttcggtt gcttcagccg ttgcgacatt gcctgcgata
      841 atttcgatct gcggaaattt ttgttttaat gttttgacca tctccaatac gccgcgcgag
      901 tgtccgtgcg cggtatccac acccaagaca tccacgcccg catccaaaag cgcttgcgcc
      961 cgttcgacgg tatctttggt tacgcccaag gcgccgccta cccgcaaacg accgtgttta
     1021 tctttgcacg catgaggata cattttcttc ttttgaatat ctttgacggt gatgagcccc
     1081 tgcaaaatat aactatcatc cacgacaaga agtttttcga tgcggtgttt gtgcagtatc
     1141 aactccgctt cctccagcgt cgtgccgact ttggcggtga ccagattatt tttggtcatc
     1201 acggtgtcaa tcgggttctg gtagttggtg gcgaaacgca gatcgcgatt ggtcagaata
     1261 ccggctaatt tgccgtcggc gtacgtgatg ggaataccgc tgattttata acgcttcatc
     1321 aaatccaacg catccgaaag tttgtgttcc ggtgtcagcg taatgggttt catgatcatt
     1381 ccgctttcgg aacgtttgac gcgatcgact tcggctgcct gatattcgat ggagcagttt
     1441 ttgtgtacga tacctatgcc gccttcgcgg gccatagcta tggccaattc cgattccgtg
     1501 accgtgtcca tcgcggcgct gatcagggga atatttaaag taatggtttt ggttaggcgg
     1561 gtattcactt tgaccaaatt gggcaatgtt tcggaataca tcggcacgat caggacatca
     1621 tcaaacgtga ggccttcgcc gacaagctta ttttcaaaat tgagcgacat aacaagctcc
     1681 ttgctaaaaa aagttgcgca atatagtcgg ctaaacctac aaacgcaagt cagagagggc
     1741 ttttttgtgc catgtacgca gcttgcatct atcgttaaaa gttatgtaca ttacatactg
     1801 ttgtgtccgc tagttattta ttggaatcag gtcttgccaa acacggtgta tggcttctaa
     1861 aatctcgacg attcgcgttg gacgtattct tgccgtagaa cgtgaggaat actttaagac
     1921 ggggattctc ttcgcatatc ttttttttat gacggcggct accatcgtcg gacgaacggc
     1981 tgcggacacg ctttttctta gccgttttga tgcgaccaag ctgtcgtaca tgtatcttgc
     2041 catctccatt gcgctcagca tatgcggcgt ggcgtttcac gccatagtgg atcgcttccg
     2101 acgcgaccgg ctgatctatg ccgttaccgg cggtatgata ttggccgtcg gggttacacg
     2161 tatttttgtg gattccggtt ggtcgctgat gtatcccatg atctatgtgg gatttgaagt
     2221 atttaacttc ctcctcatac tccagttttg gacatttacc aacgacgttt tggatacgcg
     2281 gcaggccaaa cgactgatcg gcattattct gagcgccggt attttgggcg gtatgatcag
     2341 cggtttttcg atcaaagctt tggtgcatgc catcggcacg tccaatctca ttacgatgta
     2401 cggcggcttg atggggttgt gcgtgatttt ggttcgcctg cttgcgcgcc gcgaatttac
     2461 cgatacggta cgcgaaaccc acagtgtgac cgataaaaaa gcgcataaaa aagccgaaga
     2521 aaagcataaa ctcggcagta tctatcacct caaactgatc gccatcatcg taagcatcat
     2581 cacgatggtg cttatgttta ttgattacca gtttaaaatg gtgctcaaag agtactatcg
     2641 taatgatgaa ttggcggctg tgttaggtca gttttacggc tacacgggta tcgtcgctct
     2701 ggtatttcag ttttttctga gttcgcgcat tttatcaaaa tacggtattc ttgtttcgct
     2761 tttgattctg ccgatttttt tgctgatcag ttcgctcagt atttggtttg cgccggcgat
     2821 tcccggcacg gtcatgatcg gttcattggc gctgtatctc atgccgatac tcaatgcggt
     2881 cgtttttgcg aaagcgagcg ataagatttt cagcgatacg atctattcat cggccagcca
     2941 actgatgtat gtaccgctgc atgccaacgt gcgcgggcgt gcgaaagttt ttgttgacgg
     3001 gatcatcaaa ccattatcca agagcggaag cgcgatactg ctgattttgt cggcccattt
     3061 ttttccggtc gccgtaaacg atatcagcct ggccgtagtc gggttactga tcatctgtat
     3121 catcgctatt ttcctcgtta agcgtgaata cgtcaaatcg cttttctcta cgctcaaagc
     3181 caaccagatt gatttcaaag atatcaattt taacgtgtcg gacagttcgg ccatcgagat
     3241 acttgtgcag ggcctggaaa gtcaagacga ccgtcgcgta ttgtattgtt tagacgcgct
     3301 aaaaaatatc gaaggatttt atctcatccc gcatgtgttg acattgatgc atcacaagtc
     3361 accgcgcgtg cgcaccgaag ctctgcgcgt catcgaaaaa atcacaccgc ggcaggcaac
     3421 gggcgaggtc atcaaacgtc ttgaagatga tcacaccgaa gtgaaagtgc aggctatcct
     3481 cacgctttcg gcgtatgcgg atgacaacca catcaaacgc atattatcgt ttttgattga
     3541 cgaagattcc gagatcaaag gggcggccat atcaggattg atgcagtatt acggcattga
     3601 cggtatcttt tattccgtgg atatcttcaa acagatgctc gaaagtccgc gcatcaatga
     3661 tcgtaaagaa atcgcgcgca tcatcggtcg tatcgggctg aaaaattttc ataaaccttt
     3721 gatcaaactt ctcgacgatc catccaaaga agtgcagata tttgccattg atgcggcgca
     3781 tcgcatcaat gcgatcgatc tgatcccgat gatcatcaaa aagcttaaac aaaaagaaac
     3841 acggcataaa gcgatggaag cgttgtcggc ctttgatgcg gccgatatcg taccgactct
     3901 gcgaacgtat ctggagttta gccggcagta tcaggaaatc agtttataca taccccgcgt
     3961 ttttgagatg atcgcatcgc aggaatgtgc ggacatcatg cttaaaaact acgatcacgc
     4021 cgggccgtat ctgcgtttga aaatattaga ttccttagcc aaaatacaat ttcgtaacca
     4081 cgacgtagag ttagatcgtg acttgataga aaaatggatc aatcttgaat ttcaagagca
     4141 tcgctttttt acgcaaacgt taaatgccgc aaaacccttt gatggcattg cgattttgat
     4201 cgaatccgta acgcatattc gccacggcgt gtttgaacgc gtatttaaac tgctcggctt
     4261 tttgcatgac cacaaagtga tcactacggt ttttctcaat ctgtccaata ccgacaaacg
     4321 cgtacaggcc aatgccgtcg aagtgctgga taatatgttg gagggcgaat tacgcacatc
     4381 cgtagtacaa atcgtcaatt cgcatatctc acacaagttt aacgaagagc gggatgcgat
     4441 caagtttttt gataccctga tggcggctta cggcaaagat catgattggc tcaaaatgtg
     4501 cgtggagttt gtcagcacgt cgccgaaatt tgacgaatgg gatcacaacc gtgcgtttgc
     4561 gctgcgcaaa ctcatccacg acacgcaggt cagttttgag cgcaaagaag ttctaaacat
     4621 tattcaaaaa gtcgcgatcc tcaaacgcgt cagttttttt gcctcccttt ccggtgaata
     4681 tcttgccaat atcgccctgc aaatgaaatc cgccaagtgt catgccggtg aagcggtatt
     4741 tcacgaaggt gaaacgggcg attcgtttta tgtcgttatc aaaggcaaag tattgattca
     4801 taaaggcgag acgcgtttgg tagaaatggg gccgggccaa tgtttcggtg aaatggccat
     4861 tttggataac gaaaaacgat cggccagcgt cacggcattg gaagacacgt tgatgttcaa
     4921 attggaatcc gaagcgtttt atgatattat cgccgatcgt atcgaaattg ccaaaggcgt
     4981 gatccgcatg cttacgcagc gtttgcgcca acaattggcc aaagtcgctg ccgccggcgg
     5041 gggcgctccc aaaccggtca taccgcaaat cgaagaaagc gccgtcgagg aaatgatcga
     5101 aggcgaaagc gccaatatcg cttccgaatt ggatagccag gctttactca agcgcgtgct
     5161 gctgctcaaa gaaatagatc tctttgccag tttgccggat gaagatatgg tcatgctggc
     5221 caataccatc gaagaagtga agtacaaaaa gggacaagat attttcttag aaggcgatca
     5281 gggcgatgcc atgtacggta tcgttagcgg atccgttttg atccacaaag gcaacaaaga
     5341 tttggccacc ttgggtgaaa agaaatattt cggtgaaatg gccgtgcttg ataacgaacc
     5401 gcgttccgct tcagtgaccg ctgttgccga tacgacgctt ttaaaactgt catcggatga
     5461 tttttattcg atcttgtttg ataaggtcga aatcacccgt aatattttcg ccgtattagt
     5521 acaacggctt cgtaccacca ataccaaata gatcggaatt ttttttgtct cggatacgag
     5581 tgaaaatctg ctgcatttcc tcacgcgaag aggcgcgtac ggcgattcaa accggtgcgg
     5641 atgcgttggg attggtttcg tccatgccga gcgggccggg ggttatttct gatgaaataa
     5701 tccgggatat tgcgcagatc attccccctc cgatagcttc gtttcttttg accagtaaac
     5761 aagataccgc cgcgatcgta acgcaacaaa agttttgtgg aaccaatacg attcaaatat
     5821 gtgatcattt gacggacggt tcttacgaag atatccgaaa ggctttgccg gggatcgcta
     5881 tcgtacaagt cgtaca
//
LOCUS       DAOQNQ010000206         5818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_5458_length_5818_cov_5.327087, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000206 DAOQNQ010000000
VERSION     DAOQNQ010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5458_length_5818_cov_5.327087"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..684
                     /locus_tag="PK754_11605"
     CDS             100..684
                     /locus_tag="PK754_11605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB10195.1"
                     /translation="MNSGSPVYENATIETDQILIERIRRGDEKALVTIYHNNIMMVRK
                     YVMDNSGHEDDAQDMLQDAIVVLWENVTKGQFDLTSKISTYLYSVVRNKWLREINKRK
                     IGKTSDIADYEMAEEQIDALRSVVQKEEFKTILRCIENMGATCRKLLMMFYLEEKGME
                     EIAHELKFSNTDVAKAKKWQCKKELEKMVEKAFQ"
     gene            738..1643
                     /locus_tag="PK754_11610"
     CDS             738..1643
                     /locus_tag="PK754_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10196.1"
                     /translation="MVNPTDRQWLDENEIIDKFVFGRLTDNESIRLNELRKNEDFNAM
                     FDRELMTIAALRAHGRIAMKQRIRELLHTSSPLHLSHTANPAPGKVMTMRFVWRAAAT
                     VAVIAGGLWIYKMNLQPAPEVAKKQNIQNEQAPIVNTDKQAPAQTSTEPGTPSNDVAA
                     KRTNKPNERKSMIAGQLPPSKTDRTRPAALAAVPHEVYVSISVLNATESIETRILFKN
                     PFDLVAADIRETQEEKDGQLNWFYVYYENRIVSAYLDNSKYVARFIGTHLKEEPGVLI
                     INTPQSKYRVDLTTKEKFKKAVRIP"
     gene            1646..2251
                     /locus_tag="PK754_11615"
     CDS             1646..2251
                     /locus_tag="PK754_11615"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNB10197.1"
                     /translation="MHDFTGIILAGGLSQRMGQDKFLLDFYGTPLLKNLADELAKTAR
                     EIHIVTKSSDSLAALNLHHSIITDVHPSRSAMAGIYTGLQSALYDHVFVLACDLPLFD
                     HRVTTLMQSRLPQFDTIAPKTPGGLETLCAFYHKKHLPIIDSMIRDENYRLTDFLNQT
                     KNLVLPASHFESAIYPDVFFNMNSPEDYSTALKLFKDRKSI"
     gene            2391..4457
                     /locus_tag="PK754_11620"
     CDS             2391..4457
                     /locus_tag="PK754_11620"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNB10198.1"
                     /translation="MKLFTKTLTAVCLLMLSWTFPGMAQNEMIDEDGVVADVRAKYLA
                     DSKSVLIYNGNREIPEGRTIKSDILLLNGDLIIAGTVEGKIVVSNGNIIMRETGSVTR
                     DAVSINGRVETVNSDQIAGNILQVKSSQRSMNKQDIAAKQESNFTTDESTADETSSEV
                     TAYAPSVKSKSYQENDPEELTDDETSVTPPTVQRKDGARVYDYKNKRKNKSITPYIEV
                     PPVVEAPNADNMTEADWKRFNREMEKFNRKMEKFNRKMARMNETVEKDLPEYEYNYTP
                     QTDEDDNEDEDRSHSASFVRVDNVPRRNEDRRSPRYYSRNNYWDNHQEKLRNTSFVLF
                     DYNRVDGLYIGGKIDRDHKIYDNKPFQIYGEAGYAFGPKDFHYRLGLDKIWGSEYRFT
                     LGGEVHDLTTTNDNWIVGQMENAMNSMLLKIDNRDYFRTRGFSFQAQQNLNHQFKISA
                     AYKVDDYSSASNNVNWALFRSKKIDFRTNPMIDEGRMNSVVVRAELNTRGWVYNNRKY
                     SKRVGWDIYGEAERSRDEWNSDFDFSRYVLSIARYQPLSRYENLDMRLMLGSVTGTVP
                     AQKMFHLGGISTLRGHDYKAMSGNQMALANVEYRISSGALKNDRIFPIHPFSAILFAD
                     AGYAWNNTIYAIRELARGTNLRDIQTDLGVGIGDENDLFRFDIAKSVSEKGSPYKFAF
                     RVNYIF"
     gene            4594..5760
                     /locus_tag="PK754_11625"
     CDS             4594..5760
                     /locus_tag="PK754_11625"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNB10199.1"
                     /translation="MTFRLADRFEELPVHAFAEVDRLKRESIAQGKDVIDLGVGDPDI
                     PTPQPIIDACTQALQKPANHRYPYQAGSMRFRESVAAFMKRRYDVTVDTQRNIVPLIG
                     SKEGIAHFPIAFVNPGEIVIAPDPGYPAYVTGTKLAGGVFHSIPLLAKNRFLMPLNEI
                     PADVVKRARLMFFNYPNNPTTGWADQCDFESIAQFCKTNDIIAAHDAAYQELFFEEAP
                     PSLMQTTCGLDGGIEFHSLSKTFNMTGWRVGFAVGEPSLIAGLSRLKANIDTGLFLAL
                     QEAAAFALDHCEEFTKPMIELYRRRKDTVVQGLNAAGFKSHHGKATIYVWTEVPGGSD
                     SMIFTKQLLDKTGVVVTPGSALGQSSGSYFRIALTQSEERLSEAVRRMAAFAKT"
BASE COUNT         1771 a         1283 c         1260 g         1504 t
ORIGIN      
        1 ggctcaggca agaaaagtgt atcaaaaacc ataaatattt cattattttc cgtttacctt
       61 ctttaccaaa ttgtatttat aacataagaa aaacggccca tgaattctgg ttctccggtt
      121 tacgaaaacg ctacgataga aacggatcag atactgatcg aacgtattcg ccgtggtgat
      181 gaaaaagcgc tcgtcacgat ctatcataat aatatcatga tggttcgcaa atatgtgatg
      241 gataacagcg gccatgaaga cgacgcgcag gatatgctgc aggatgccat cgttgttctt
      301 tgggaaaatg taacaaaagg ccagttcgac ttgacttcca agatcagcac gtacctttat
      361 tctgtggtac gaaataaatg gctgcgggaa atcaataaac gcaaaatcgg caaaacaagc
      421 gatattgccg attacgaaat ggcggaagaa cagattgatg cactgcgcag tgtggtccaa
      481 aaggaagaat ttaagaccat tctgcgttgt atcgaaaaca tgggcgctac gtgccgtaaa
      541 ctgttgatga tgttttatct agaagaaaaa ggcatggaag aaatagccca tgagctaaaa
      601 ttcagcaata cagatgtcgc caaggccaaa aaatggcaat gcaaaaaaga actcgaaaaa
      661 atggtcgaaa aagcatttca ataaaacaaa aactaagcac aagtaaaaac aaataacacg
      721 aaatgatgcg atagaatatg gtaaatccga ccgaccgcca atggcttgat gaaaatgaaa
      781 ttatagataa gtttgtcttc ggcagactga cggataacga atctatccgt ctgaatgagt
      841 tacgtaaaaa cgaagatttt aacgcgatgt ttgatcgcga attaatgacc atagccgctt
      901 tgcgtgcaca cggtcgcata gccatgaaac aacgcattag agaattgctc catacatctt
      961 ctccgctaca tttatcacat accgcaaatc cggcacccgg aaaagtaatg acgatgcgtt
     1021 ttgtgtggcg tgctgctgcg accgtagctg tgatcgccgg aggactctgg atttataaaa
     1081 tgaatcttca accggcaccg gaagttgcta aaaaacaaaa tattcaaaac gagcaagcac
     1141 cgattgtaaa cacggacaaa caggctcctg ctcaaacgtc taccgagccg ggtactccat
     1201 ccaatgatgt agccgcaaaa cgcaccaata aacccaacga acgcaagagc atgatcgccg
     1261 gtcagttacc tccgagtaaa acggatcgta cacgtccggc cgctctagct gctgttccgc
     1321 atgaagtgta cgtgtcgatt tcagttttga atgcgacgga atccatagaa acccgtattt
     1381 tatttaaaaa tccgttcgac ttggttgcgg cggatatacg cgaaacacaa gaagaaaagg
     1441 acgggcagct caactggttt tatgtgtatt acgaaaatcg tatcgttagc gcatatttgg
     1501 acaattctaa atatgtagca agatttatcg gaacacatct gaaggaagaa cccggggtct
     1561 tgataataaa tacgcctcaa tccaaatatc gcgttgatct tacgactaaa gaaaaattca
     1621 aaaaagccgt acgtattcct taaccatgca tgattttacg ggcattattc tggccggcgg
     1681 tctatcccaa cgaatgggtc aggataagtt tcttctcgac ttttacggta ccccccttct
     1741 taagaattta gctgacgaat tggctaaaac cgcacgcgaa atccatatcg taactaaatc
     1801 ctccgactcc ttagcagcgc ttaatctaca tcactcaatc atcacggatg tgcatccgtc
     1861 gcgatcagcg atggcaggaa tctataccgg acttcaatcc gcattatatg accatgtatt
     1921 tgttttagct tgtgatttac ccctttttga tcatcgtgta accactctga tgcaatccag
     1981 attacctcag tttgatacga tcgcccccaa aacacccggc gggcttgaaa cgctatgtgc
     2041 attttaccac aaaaaacatc ttcctataat tgattcgatg atccgcgacg aaaattaccg
     2101 cttaacggat ttcctgaatc aaaccaaaaa cctcgttctg ccggcctcac attttgaatc
     2161 tgcaatttac cctgacgttt tttttaacat gaatagtccg gaagattatt cgacagcatt
     2221 aaaactattt aaagatcgaa aaagcatcta aaaagggaat ataaagcata tataattgca
     2281 acttttttag ggttaaacgg tcttttttag aaaatataaa aatcctgcaa ctttttggga
     2341 ttaggcaccg taaaaaacaa agctaaccat ataagagata aggggcgacc atgaaacttt
     2401 tcacgaaaac tttaacagca gtttgtttat tgatgcttag ctggacattt ccaggtatgg
     2461 cacaaaatga aatgattgac gaagacggtg ttgttgcaga tgtgcgtgcg aaatatttgg
     2521 ccgattccaa atcggtcctg atatataacg gtaaccgtga aattcccgaa gggcgcacga
     2581 tcaaaagcga tattttactg ctcaacggcg accttattat tgccggtact gtggaaggta
     2641 aaattgtcgt ttccaacgga aatatcatca tgcgcgaaac cggttcggtc actcgtgatg
     2701 ccgtcagtat caacggccgc gtcgaaacgg ttaattccga tcagattgca ggcaacattc
     2761 tgcaggtaaa gtcctctcag cgctcaatga ataaacaaga tatcgccgca aaacaagaat
     2821 ctaattttac aacagatgaa tctacagcag atgaaacatc aagtgaagta acggcttatg
     2881 cgccatccgt caaatcgaaa agctatcaag agaatgatcc cgaagaatta accgatgatg
     2941 aaacctccgt aacaccacct actgtccaac gtaaggacgg tgcgcgggtg tatgactata
     3001 aaaacaaacg taagaataaa agtattaccc cgtacatcga agtgcctcct gtggtagaag
     3061 ctcctaacgc ggacaacatg accgaagcgg attggaagcg gttcaatcgc gagatggaaa
     3121 aatttaatcg taaaatggaa aaatttaacc gcaaaatggc gcggatgaat gaaaccgtcg
     3181 aaaaagatct gccggaatac gaatataact atactcccca gacggatgaa gacgataatg
     3241 aagacgaaga tcgttcgcac agcgcttcgt ttgtacgggt ggataatgta cctcgccgta
     3301 atgaagatcg tagaagtccg cgctattatt cacgcaataa ttattgggac aatcatcagg
     3361 aaaaacttcg caacacatcc ttcgttcttt ttgactacaa tcgtgtagat ggtctttata
     3421 tcggcggcaa aatagatcgt gaccataaga tatatgataa caaaccgttt caaatatatg
     3481 gtgaagccgg ttatgctttc ggacctaaag atttccatta ccgccttggt ctggataaaa
     3541 tctggggttc ggaataccga tttactttag gcggtgaagt gcacgacctg accacgacta
     3601 acgataattg gattgttggg caaatggaaa atgctatgaa ttccatgctg ttaaaaatag
     3661 acaatcggga ttatttccgt acacgtggtt ttagtttcca agctcagcaa aacctgaacc
     3721 atcaattcaa aataagcgca gcctacaaag ttgatgacta ctcgtccgct tccaataatg
     3781 tgaattgggc gctttttcgg agtaaaaaaa ttgacttccg aacaaaccct atgattgatg
     3841 aaggtcgtat gaacagcgtt gttgtgcgtg ctgaactcaa tacgcgcgga tgggtctata
     3901 ataaccgcaa gtacagcaaa cgtgttggat gggatattta cggcgaagcc gagcgtagcc
     3961 gcgatgagtg gaattccgat tttgattttt cccgctacgt tctgagcatc gcacgctacc
     4021 aaccgctgag ccgttacgaa aatctggata tgcgcctgat gttgggatcg gtgaccggca
     4081 cagtccccgc tcagaaaatg tttcacctcg gcggcatctc caccttacgc ggtcatgatt
     4141 ataaagcgat gtcgggaaat caaatggcat tggctaatgt cgaataccgc atcagttccg
     4201 gtgctctgaa aaatgatcgc attttcccca ttcatccgtt cagtgccatt ttatttgccg
     4261 acgccggtta tgcatggaat aatacgattt atgccattcg cgaattggct cgtggaacca
     4321 atctgcgcga tattcaaacc gacctcggcg taggtattgg cgatgaaaat gacctgttcc
     4381 gatttgatat cgccaaatcg gtaagcgaaa aaggcagtcc ttacaaattt gcattccgcg
     4441 tgaattacat tttctgattt gatacaaact taccgtacaa ccccgcttcg tcatccgatg
     4501 cggggttttt attttacatg aattatcagg cttgaatgaa aatatccttt acggtatatt
     4561 gttcagattt tttttgaact tactattatt tttatgacat ttcgtttagc tgatcgtttt
     4621 gaagaattgc ctgtacatgc ttttgccgag gtggatcgcc tcaaacgtga atccattgcc
     4681 caaggaaaag acgttataga tctgggtgta ggtgaccccg atattccaac cccacaacct
     4741 atcatagatg cttgcactca ggcgctacaa aagccggcca atcaccgata cccctaccaa
     4801 gccggctcga tgcgttttag agaaagtgtt gcggctttta tgaaaagacg atacgatgtc
     4861 acggtagata ctcagcgcaa tatcgttcct ttgatcggct ccaaagaagg tattgcccat
     4921 tttccgatcg cttttgtaaa ccctggtgaa atcgtcattg caccggatcc cggttatccg
     4981 gcctatgtaa caggaaccaa attagccggc ggcgtgttcc attcgatacc gttattagca
     5041 aaaaatcgtt ttctgatgcc gctcaatgaa atcccagccg atgtcgtaaa acgtgcacgg
     5101 ttgatgtttt tcaattatcc caacaacccg actaccggtt gggcggatca atgtgatttc
     5161 gaatccattg ctcagttctg taaaactaac gacatcatcg ctgcgcatga cgcggcatat
     5221 caggaacttt ttttcgaaga agcgccaccc agtctgatgc aaacaacatg cgggcttgac
     5281 ggtggtattg aattccattc gctgtctaaa acttttaaca tgaccggatg gcgcgtcgga
     5341 tttgccgtcg gcgaaccttc cttgatcgcc ggactgtccc gactcaaagc caacatagat
     5401 accggtttat tcttagcgtt acaagaggcg gccgcgtttg cattggatca ttgcgaagaa
     5461 tttacgaaac ctatgatcga attatataga cgccgcaaag atacggtagt gcaagggctc
     5521 aatgcggccg gattcaaaag tcatcatggc aaagcaacca tttacgtatg gacagaagtg
     5581 cccggcggtt cggattcaat gatctttact aaacagttat tggataaaac aggagtcgtt
     5641 gtgacacccg gttcggcgct tggtcagtcc agcggatcgt actttcggat tgcgctgacg
     5701 cagtccgaag agcgcctctc ggaagccgtg cggcgtatgg cggcttttgc taaaacataa
     5761 catgaaaaat aacgataaaa tccgactcgt caatctgatc atttatgctc atcatggt
//
LOCUS       DAOQNQ010000207         5762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_5543_length_5762_cov_6.015770, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000207 DAOQNQ010000000
VERSION     DAOQNQ010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5543_length_5762_cov_6.015770"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PK754_11630"
     CDS             complement(<1..480)
                     /locus_tag="PK754_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB10200.1"
                     /translation="MIRLQHISKQFGRFKALDDLSIEIPSGDFMALLGPNGAGKTTTL
                     KLLAGLIRPSSGAIQVDGMEKNSRSVFLQSIGMVSHQSFLYNDLTAIENLRFYANMYT
                     VSNASARIASLLKRVGLGARRDDIVRAFSRGMLQRLSIARALLHEPQMLLLDEPYTGL
                     "
     gene            complement(477..1655)
                     /locus_tag="PK754_11635"
     CDS             complement(477..1655)
                     /locus_tag="PK754_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10201.1"
                     /translation="MKRWLLFMALSALLVPRSFFAQASVSGKVILGSTKQPVKADWVT
                     LIKSGQGLQTLQQLDNVSEYAFKDIPLNPMAPYLIRASYKGILYTQNVMINENKPYIA
                     DVSVAGVSDQWRDIDVRVPHMIVARTDDKLEIEQTFEITNNGANTFNPKDTNVPSFLF
                     KLPDGVKLEAVTTSFDKSMPIPASVVNYKDKQGIIFPLRPGTTQIQITASAPYASPSL
                     TWAQPMYYDVSEFRIFVTPKDIEVSGENFQHIHDEQLEANNFSITAATNLKSGSTIGI
                     SFKGGSARGGAPQEDDHPIDAAANEIQRNVFFLAPLLMSFLLFALYIALNRKHQSDGG
                     GAQQKAHWMAEQEKLIASIARLDDEYAENSDNSEYVDRRRDLKHQLRDVSKRIQRAGA
                     "
     gene            complement(1675..2121)
                     /locus_tag="PK754_11640"
     CDS             complement(1675..2121)
                     /locus_tag="PK754_11640"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNB10202.1"
                     /translation="MLSLLMILLSAATFGYVLYPLFSKNVMPYGLKPIKDQHRDDLAN
                     MKQKLYTDIKDLDFEYGIGKIAEADYQNLRQATLKELADVLKRMDAIEQGDNGNGKIS
                     DEYLEHLIQSRRAKTIVVQKAICPSCQHENMKTAKFCSECGAKLNA"
     gene            complement(2125..2649)
                     /locus_tag="PK754_11645"
     CDS             complement(2125..2649)
                     /locus_tag="PK754_11645"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HNB10203.1"
                     /translation="MKKLKIYFIASILITSALYSQGGSAHTTKLNAEQNEMFNKISGE
                     LICQCGCNLVLGTCGHINCPSAVPMRNTIEDMIVAGKSHDAIIQYFMTEYSFRDNPPA
                     GKAILSQPETKGFDLLAWIMPFLLFVIFIIVVVFLIKKITAGKTAPVTVSPAHPADDP
                     YTQRIEEELKKMDR"
     gene            complement(2636..4651)
                     /locus_tag="PK754_11650"
     CDS             complement(2636..4651)
                     /locus_tag="PK754_11650"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HNB10204.1"
                     /translation="MGQIGNYALIVALAMAAFAILSAFLGVHYKRGDLVRSAQNAVYV
                     VFAMVVVAAGSLIYLMVTGDMTYEYVASYTNRDLPLFYKYTFWAGQAGSLLLWSLLLA
                     AYSFAVVYRYRNSFGELMPWVIVVTMATQVFFLALNLFSSNPFDVLVTHHGDGSETLF
                     RPQDGRGLNPLLQHPAMVIHPPTLFVGYVGFAIPFAFAMAALISGQLNDEWIKLSRRW
                     TLFAWLFQSAGIMMGGWWAYEELGWGGYWAWDPVENASLMPWLTGTAFLHSVMIQEKK
                     NMFKVWNVSLIIATYLLCIFGTFLTRSGVVSSVHAFAQGSIGIYFISFITLSLLVSVW
                     FVIKRLPMLKSEGQLDSALSRESSFLFNNLVLLISCFAVLWGTVFPIISEAVTGEKIV
                     VGAPFFNKVNIPIALFLLFLTGAGPLFAWRKTSAASLRKAFLLPTIIALAGGVVIFMF
                     VTQHFYGLMSFVLNIFVAAVIAIEFAKGTSARMRSNGENVLIALKNLTLKNKRRYGGY
                     IVHFGIVVIFVGITGSIFNYETKTELGQGESRQVKDYLLKIDKVDFTNTPNYQSSYAV
                     VSVYKDDKKIDELKPEYRMYRSSQQPTTEVALHKTLKEDLYLVFTGLDDTEKKAVLQI
                     YVNPLVSLVWLGTEILVFGTLICLLPAYQEDRKSVKSMRKEKTLEKA"
     gene            complement(4698..>5762)
                     /locus_tag="PK754_11655"
     CDS             complement(4698..>5762)
                     /locus_tag="PK754_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNB10205.1"
                     /translation="LDQAMIRKTSSDPFQFTIHGERYLAVAHPVFAATGSQGQERPVI
                     ATYVFFSSLDKALEPLVASQRTMVFVGGGVTILAFMIALVIISGVTKPVHQLSDAMHK
                     VQSGDLNIMLQIKSRDEFGELGKNFNEMTQGLKQKERVENLFGKYLSPDVAKKVLSEQ
                     SVDGILKGEKNRLSVMFTDIRGFTPMSRGMDPQELINLLNSHFDEMIDIIDSYGGTLD
                     KFIGDAIMAFYGAPVHYDDFYMRAINAAVQMQRAAEKFNFQRKLEGHPPINIGIGINT
                     GEVVVGNIGSNKRLEYTVIGETVNIANRLCSVAKAGQIIVSQSTYDLLPNKSIASPIE
                     RVTLKGVAEPVTVYEILWKS"
BASE COUNT         1660 a         1313 c         1299 g         1490 t
ORIGIN      
        1 caggccggta tagggttcat ccaaaagcaa catttgcggt tcatgcaaaa gtgcccgtgc
       61 aatagacagg cgctgcagca tgccgcgtga gaaggcgcga actatatcat cacgccgcgc
      121 acccaatccg acacgtttta aaagtgaggc gatacgtgca gaggcattgg aaacagtata
      181 catattagcg taaaaacgca aattttcaat agccgtcaga tcattgtaca aaaaagattg
      241 gtgggatacc atgccgatgg attgtaaaaa aacggagcga ctgttttttt ccataccgtc
      301 cacctggatg gcgccggaag aaggtcggat caatcctgca agtagtttga gcgtggtcgt
      361 tttgcctgca ccgttaggtc ctagcaacgc catgaaatca cccgaaggga tttctatcga
      421 aaggtcatcc agcgctttaa aacggccaaa ttgtttcgaa atgtgctgta atcggatcat
      481 gcgccggcac gttggatgcg ctttgaaaca tcgcgcaact gatgtttgag atcgcgtcgc
      541 cgatctacat actcactgtt gtccgaattc tcagcatatt catcatctaa tcgagcgatg
      601 gatgcgatca gtttttcttg ctcggccatc cagtgtgctt tttgttgcgc accgccgcca
      661 tcggattgat gttttcgatt gagtgcgata tacaaagcaa aaagaagaaa tgacatcaaa
      721 aggggagcaa ggaagaacac attgcgctga atttcgttgg cagcggcatc tatcgggtga
      781 tcatcttctt gtggcgctcc gccgcgcgca ctgccgcctt tgaagctaat acctatcgta
      841 cttccggatt tcaggttagt agcagccgta atcgaaaagt tatttgcttc caattgttca
      901 tcatgaatat gttggaaatt ctcaccgctg acttcgatgt ctttgggcgt gacgaagata
      961 cgaaattccg atacatcgta atacataggt tgcgcccatg ttaaagacgg cgaagcatac
     1021 ggcgccgatg ccgtgatttg tatctgtgta gtgccggggc gtaatggaaa aatgatacct
     1081 tgcttgtctt tatagtttac caccgaagca ggtattggca tgcttttgtc aaaagaggtg
     1141 gtaacggctt ccaattttac cccgtccgga agcttaaaca aaaatgaagg gacattggta
     1201 tctttgggat taaaagtatt ggcgccgtta ttagttattt caaaagtttg ttcgatttcc
     1261 agtttatcat cggtacgagc gacgatcata tgcggaacgc gaacatctat atcgcgccat
     1321 tgatcgctga caccggctac ggatacatcg gcgatatacg gtttgttttc attgatcatc
     1381 acattttgtg tgtataggat gcctttgtat gaagcccgaa taagatacgg agccatagga
     1441 ttgagcggaa tatctttgaa agcgtattcc gatacattgt ctaattgctg caatgtctgt
     1501 aatccttggc cggatttgat gagtgttacc caatctgctt ttacaggttg tttggtcgaa
     1561 ccgagtataa ctttaccgct taccgaggcc tgcgcaaaaa aactgcgggg gacaagtaaa
     1621 gccgataaag ccataaagag aagccagcgt ttcatatacg attccatagg ataattatgc
     1681 attcagtttg gctccgcatt cggagcaaaa tttagccgtt ttcatgtttt catgttggca
     1741 agaaggacat attgcttttt gcacaacgat ggttttggcg cgtcgggatt gtatgagatg
     1801 ctccaggtat tcgtccgata tttttccatt accgttgtcg ccttgttcga tggcatccat
     1861 tcgtttgaga acatcggcga gttctttgag cgtagcttga cgcaagtttt ggtaatcggc
     1921 ctcggcgatt ttgccgatac cgtactcaaa atccaaatcc ttgatgtcgg tataaagttt
     1981 ttgcttcata ttagccagat cgtcgcgatg ctgatcctta atcggtttga gtccgtaagg
     2041 catcacattt ttcgaaaaaa gcggatacag tacatatccg aaagttgcgg cagaaagtaa
     2101 aatcatcaat aacgataaca taaatcaccg atccattttt ttgagttctt cttcgatacg
     2161 ttgcgtgtac gggtcgtcgg cgggatgggc cggtgaaacc gtaaccggag ccgttttacc
     2221 ggccgtaatt ttttttatga gaaaaaccac aacgatgata aatataacga acaacagaaa
     2281 aggcatgatc cacgccagaa gatcaaatcc tttcgtttca ggttgtgaaa gaatagcttt
     2341 tcccgccggg ggattgtcgc gaaacgaata ttcggtcata aaatattgga tgattgcatc
     2401 gtgggattta cctgcaacga tcatgtcttc gatcgtattg cgcatcggca cggccgaagg
     2461 gcagttgata tgcccgcaag tcccgagcac cagattgcaa ccgcattggc aaatcagttc
     2521 gcccgatatc ttattaaaca tttcattttg ttcggcatta agtttagtcg tgtgtgccga
     2581 cccgccttgg gaataaagtg cggatgttat taaaatcgaa gcgataaaat aaatcttaag
     2641 ctttttcaag cgtcttttcc tttcgcatag atttgacgga tttgcgatct tcctggtaag
     2701 cggggagcaa acaaatcaaa gttccaaaaa caagtatctc agtgccaagc caaaccaatg
     2761 aaactaacgg attgacataa atctgtaaaa cagctttttt ctcggtatca tccagcccgg
     2821 taaaaacgag atataaatct tctttgagtg ttttgtgtaa cgccacttcc gtcgtaggtt
     2881 gttgactgga gcgatacata cgatactcgg gtttcaattc atcaatcttc ttatcatctt
     2941 tatatacgga aacgaccgca taagaggatt gatagttcgg cgtattggtg aagtccactt
     3001 tatcaatctt caaaaggtaa tctttcacct gccgggattc gccctgaccc agttccgttt
     3061 tggtttcata gttaaaaatg ctgcccgtaa tacctacgaa aatcacgaca atgccgaagt
     3121 gtacgatgta gcctccgtaa cggcgcttgt ttttgagggt taagtttttg agtgcgatta
     3181 acacgttttc accattggaa cgcatacgcg cacttgttcc tttggcaaac tcaatcgcaa
     3241 taaccgccgc aacaaaaata ttgagcacaa acgacattaa tccgtaaaaa tgctgcgtaa
     3301 caaacataaa tatgacaaca ccgccggcca aagcaatgat cgtgggtaat aagaaagctt
     3361 ttcgcaaact cgctgctgaa gtcttacgcc atgcaaagag cggtcccgcg ccggtaagga
     3421 acagaagaaa taatgctatc ggaatattta ctttgttgaa aaacggcgcg cctacgacaa
     3481 ttttttcacc ggtgacggct tctgaaatga tagggaatac cgtaccccac aatacagcaa
     3541 aacacgaaat tagaagtacg aggttattaa acaaaaaact actttcacgc gataatgccg
     3601 aatcaagctg accttcgctc ttgagcattg gcaaacgctt gatcacaaac caaaccgaaa
     3661 ccaataagga caatgtgatg aaggaaataa aatagattcc tatcgaacct tgcgcaaagg
     3721 cgtgtacaga cgaaacaacg ccggaacggg tgaggaatgt accgaaaata cacagaaggt
     3781 acgtggcaat gatcaaagaa acgttccata ctttgaacat attctttttt tcctgaatca
     3841 tcaccgaatg taaaaaagcc gttcctgtaa gccagggcat cagcgatgca ttttcaaccg
     3901 gatcccaagc ccaatatccg ccccaaccga gctcttcgta agcccaccaa ccgcccatca
     3961 tgatgccggc gctttgaaaa agccacgcaa aaagagtcca gcggcgcgat agtttgatcc
     4021 attcgtcatt gagttgtccg gaaatcaaag ccgccatagc aaaagcaaac ggtatcgcaa
     4081 agccgacata acccacaaaa agtgtaggtg ggtggatcac catcgcggga tgctgcaaaa
     4141 gcggatttaa accacgaccg tcctgcgggc gaaacaaggt ttcgctcccg tcgccatgat
     4201 gcgtcacaag gacatcaaat gggttggaag aaaaaagatt gagagccaga aaaaaaactt
     4261 gtgtggccat cgtaacgacg ataacccacg gcatgagttc gccgaaacta ttgcggtatc
     4321 ggtacacgac ggcaaaacta tatgccgcaa gcaacagcga ccacaaaagc agtgatccgg
     4381 cctgacccgc ccaaaatgta tatttgtaaa aaagcggcaa gtcccggtta gtgtacgatg
     4441 cgacatattc gtatgtcata tcaccggtaa ccatcaaata aatcaaactt ccggcggcaa
     4501 cgacaaccat tgcaaaaacg acatacacgg cgttttgtgc cgaacgtacc aaatcaccgc
     4561 gcttgtaatg aacgccgaga aacgccgaca agatggcaaa agcagccatc gccagcgcta
     4621 cgatcaacgc ataattacct atttgaccca tatatattcc ttagcttatt tgacagacga
     4681 tgtatacgct atttttatta acttttccat agaatttcat acacagttac cggttcggca
     4741 acgcctttga gcgtaacgcg ttcgatcggc gaggcgatgc ttttattggg caaaagatca
     4801 tacgtggact gactgacgat gatctgtccg gctttagcga cgctgcacaa acgattggca
     4861 atgttgacgg tttcaccgat cacggtatat tcaaggcgtt tattggagcc gatattgcct
     4921 acaacgacct cgccggtatt gatgccgata ccgatattga tcggcggatg accttctaat
     4981 ttgcgttgaa aattaaattt ctcagccgcg cgttgcatct gtacggcggc attgatggcg
     5041 cgcatataaa aatcatcgta gtgaaccggc gctccgtaaa atgccatgat agcatccccg
     5101 ataaatttat ccaacgtacc accataactg tcaataatgt ctatcatttc atcaaagtgc
     5161 gaattgagca aattaataag ttcctgtggg tccataccgc gcgacatcgg cgtaaatccg
     5221 cgaatgtcgg taaacataac gctgagccga ttcttttcgc ctttgagaat accatcaacg
     5281 gattgttcgg acaaaacttt tttggcaacg tcaggggaaa gatatttacc gaaaagattt
     5341 tcgacacgtt ctttttgctt gagtccttgc gtcatctcgt taaaattttt acccagttcg
     5401 ccgaactcat cgcgcgattt gatctgcaac atgatattga gatcaccgga ttgtactttg
     5461 tgcatagcgt cggaaagctg atgtacaggc ttggtcacgc cgctgataat caccaaagca
     5521 atcataaaag ccaaaatggt aacaccgcca ccgacaaaaa ccatcgtgcg ctgcgaagcg
     5581 acgagcggct ctaaggcttt gtcgagtgaa ctaaaaaaaa catacgttgc aataaccggg
     5641 cgctcctgac cctggcttcc cgtagctgca aaaacaggat gggccacagc caaataacgc
     5701 tcaccatgaa tcgtaaactg aaaaggatcg cttgaggttt tacgaatcat cgcctgatct
     5761 aa
//
LOCUS       DAOQNQ010000208         5754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_5552_length_5754_cov_4.750658, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000208 DAOQNQ010000000
VERSION     DAOQNQ010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5754
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5552_length_5754_cov_4.750658"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..3035
                     /locus_tag="PK754_11660"
     CDS             162..3035
                     /locus_tag="PK754_11660"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HNB10206.1"
                     /translation="MNLSIRTRVSCGRLLKTALCGLSLTVVTGTGTAQEKEARAEYRA
                     SVKSYINSLDESFAYRYLDNEVMILDLMSTTREEFWYRRDKKIAIPPGTEGLPFARKE
                     MRDSIRLRMNTIKENLEGMKKSNRVLPGYTDQKALNELHMEYQRLYAMDLGMTKIDAM
                     AIRSRLVKSGTPDDIRRMVQHHLKIALRRYKEESYSAAAAELQDIYETYNTVLNEWDD
                     VLYYIGDSYYRMKDFDNAKTWFDKLVSEMTTSPYVERALYTQITMAYIKEDRSAMQKY
                     YGEYLAKAPERPKDDNRYNRAFYLAGLTFFSTSDYTTGIAALRKVSKESRYYWPARYL
                     VAHCYVGLEQYNEALEEFVHVTKIEPNNPRFTVEDQKRLKDLSLLKASFIKFEQTING
                     QKFNAVFPYMKKIQRSSEYHDAALLIASWASFKDNDIDTARIFADSLVNLYPASDYLF
                     ETKTLLGNIKVLDPTLTDRDRELYAVESYNFVAEATEAKYLADQFIAERDSTRKTLMM
                     LEDAKRVARIKNDSASFMRYDELHRQLSDALENNGYNRIREGKSAGFYQTMSVMASKI
                     RVTEAKLREARESGNKEDEEKFGKQLSDQISDFQQIGAENYLEKNRIDASEGSTDQLA
                     ALEIETYFQKHKISRTISEMEARNRQQSVLREKIGREKQLVQNQLFQIDDLMKTARER
                     NNQSAMLKLDFERNKLAGLYYRLSDYEVLLLADAPAESYVDLDTWGDFASYGRNNINF
                     VLNSTKSEQIGDIGKAVAQIDKILETRKRNYELRIAEVEREIELKEREIRDKELQEMR
                     REQQTFFQKEYFLDKSSEKPENDPFDYNDVVPEVIVIDTKPIEAPQEQQQEEGAGDEE
                     LPPSETVQGDSTVKSTPADSASSTSAPGTQTKEENATADSTSGDGSNGESALLINNEA
                     AQGVQPTFILRNQVADVAGKPRNAFVFIRRDEKSIQSKKIFKS"
     gene            3047..>5754
                     /locus_tag="PK754_11665"
     CDS             3047..>5754
                     /locus_tag="PK754_11665"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB10207.1"
                     /translation="MTLVLRSGKWIALVLLAGLVSQTALYAQRGTGNKSNKDTVALES
                     VSSEDLLKLKEELESQRNKLETERVQLLDKGIKQSKEFLDKSGSGTATTALVLLQRAE
                     YLYQIMNDNFSVVSDSIATENQKILNEYERRKEDFRTNLVAQKKTDKEIEQEMNQYPG
                     PQLLPDPEMDYTEVIKTYQQLMDNFPESPYVVDAMYNIAFFREAEGRQLKSRGDERFI
                     NDGDRRQREALKIYQDLAVRFPDSKYAGECYNRIGEYYFQRGGDADLQKAIKNYSKVL
                     DYPQAERFQEAVYKLAWTHYRLGDYPKAISYFTYLVDDVDSARYYNNFSQELDVEALV
                     YIGISFNRWGEQIDLAQGTADGGYKLIKSYIDEAKLSEKRYAPEIIWQLGESYNLEQK
                     DTLALYAYNTLINTYPLFWRTADAQFKVINTFERMQRGTQNKAVAKSLLDSVIFHRYK
                     LYNSYRPGSDWSVAMTDKDLVIRANRMARDVLVDNILYYYGEASLSNDLKDWRVAMDF
                     SRQFITFFPVDTFAYKFHYNMAFIEFAFFKMLDTAYEDFVKVATLYPYDQYRFKSAMN
                     AYVIADSLYRARPFARPKDVPMDSILPITPSESKLLDAINNYARLFPDTSALYPTDSL
                     ENPKPVMTKPGYKTPDFLAYAGGIYFSHNDFARASQYFNTIVTRYPKSDKARLAQRYL
                     MQTYRDKKDFRSSEIVARKLLENSEVSAEQKTEAVQVIFFSIFKHAEYFQGKKENYKA
                     AREFQRAYEEGKKLGYGKRNELAIAMFNSGVEYYNSKELKRSIAVFEAYADTFKDTKE
                     APAALWNVQNMYSDMKEMKTAAQVGERLVDRYPNYSDGSRSSEVALYNSEYFFEQSSK
                     QSAAKGDSNEAKDLNRQAIRVSEKFVKLFPKSQYTPELDFNIAKLY"
BASE COUNT         1733 a         1208 c         1371 g         1442 t
ORIGIN      
        1 atacggaaaa gtaagatcga ccaacaaagt gtaaaccaat aaaataactt gacttcactt
       61 tttaaatcgc ctaaattcgc tgaaattaga taaaacaaat ccttaggtta aattccaaag
      121 gccgacgacg gtcttttgct aatcttcaca ggaaggtcgg aatgaatttg agtattcgca
      181 cccgcgtatc gtgtggccgt ttattgaaga ccgcgctctg cggactgagt ttgacagtgg
      241 tgaccggtac tggcacggcg caagaaaaag aagcccgcgc tgaataccgt gcgagcgtca
      301 aaagctacat aaacagcctc gacgaaagtt ttgcgtatcg ttaccttgat aacgaggtga
      361 tgattcttga cctgatgtct acgacgcgtg aagagttttg gtaccgtcgt gataaaaaaa
      421 ttgcaattcc gcccggtacg gaagggttgc cttttgcacg caaagaaatg cgtgactcga
      481 ttcgtttgcg catgaatacc atcaaagaaa atcttgaggg tatgaaaaag agtaatcgcg
      541 ttcttcccgg atataccgat cagaaagcat tgaatgagct ccacatggag tatcagcgtt
      601 tgtatgcgat ggatcttggt atgaccaaaa tagacgcgat ggctattcgc tcccgattgg
      661 tcaagagcgg aacacccgat gatatacgtc gcatggtcca acaccacctc aaaatcgcac
      721 tgcgccgcta taaggaagaa agttactcgg ccgcagctgc ggagttgcag gatatttatg
      781 agacgtacaa tactgttctc aacgaatggg atgatgtgct ttattatatc ggtgacagtt
      841 attaccggat gaaagatttt gataacgcga aaacctggtt cgataaattg gtttccgaga
      901 tgaccacctc gccatacgta gaacgggcat tgtacacaca gattacgatg gcgtacatca
      961 aagaagatcg ttccgcgatg caaaaatact acggcgaata tctcgctaaa gctccggaac
     1021 gtcctaaaga tgataaccgt tacaaccgag ctttttattt ggccggtttg acattttttt
     1081 ccacttcaga ttatacaacc gggatagccg cactgcgcaa agtatccaaa gaatcgcgtt
     1141 actattggcc tgcgcgatat ttggtagcgc attgctatgt tgggttggaa caatataatg
     1201 aagcgctgga agaatttgtt catgttacga aaatcgaacc gaacaatccg cgatttaccg
     1261 tagaagatca aaaacgcctg aaagatctct cgttgctcaa agcatctttc atcaagttcg
     1321 agcaaacgat caacggacaa aaattcaacg ccgtatttcc gtacatgaaa aaaatacagc
     1381 gcagttcgga atatcatgat gccgcactgc tgatcgcgtc ttgggcttcg tttaaagaca
     1441 atgatattga tacggcgcgc atattcgcgg attcgttggt taacttatat cctgccagcg
     1501 actatttatt tgaaaccaaa acactgcttg gtaatatcaa agtgttggat cctacgctga
     1561 cggatcgcga tcgcgagtta tatgcggtcg aatcatataa ttttgtcgcg gaggctacgg
     1621 aagccaagta tctcgcggat cagtttatcg ctgaacgcga ttcgacacgc aaaacactga
     1681 tgatgctcga agatgcgaaa cgtgtcgcgc gcatcaaaaa tgatagcgcg agctttatgc
     1741 ggtatgacga gttgcatcgc caactcagtg acgcattgga aaacaacggt tataaccgta
     1801 ttcgcgaagg caagtcggcc ggtttttatc aaactatgtc ggtcatggcc agcaaaattc
     1861 gcgtaacaga agccaagttg cgcgaagcgc gtgaaagcgg caacaaggaa gacgaagaaa
     1921 aattcggcaa acaactttcg gatcagattt cggattttca gcaaatcggc gctgaaaact
     1981 atctggaaaa aaatcgcata gatgcatcgg aaggctcaac ggatcagtta gccgcgcttg
     2041 aaattgaaac gtattttcag aaacataaaa tttcgcgaac gataagcgag atggaagcgc
     2101 gtaaccgtca gcagagtgtt ctgcgcgaaa aaatcgggcg tgaaaaacag ctcgtacaaa
     2161 atcaactttt ccaaattgat gatctgatga aaacagcgcg cgaacggaac aaccaaagtg
     2221 cgatgcttaa gctggatttt gaacgtaata aactggccgg attgtattac cgcttaagcg
     2281 attacgaagt tctgctttta gccgacgcac ctgccgaaag ctatgtagac ttggatacct
     2341 ggggtgattt tgcgagctac ggtcgcaata atatcaactt cgtccttaat tcgacgaaat
     2401 cggaacagat cggcgatatc ggaaaagcgg ttgcacagat tgataaaata ttagaaacac
     2461 gaaaaagaaa ttatgagctt cgtattgcgg aagtggagcg cgagatcgaa ttgaaggaac
     2521 gggaaattcg cgataaagaa cttcaagaga tgcgtcgcga acagcagacg ttttttcaaa
     2581 aagaatattt cctcgataag tccagcgaaa aacctgaaaa cgatccgttt gattataatg
     2641 acgtggtgcc ggaagttatc gtaattgata ccaagccgat tgaggcgccg caggaacagc
     2701 aacaagaaga aggtgcgggg gatgaagaat tgccgccttc ggaaacggta caaggcgact
     2761 cgacggtgaa gtcaaccccg gctgattcgg cctcgagtac ctctgcgccg ggcactcaaa
     2821 ccaaagaaga aaatgcgacg gccgattcga catccggtga cggttctaat ggggagtctg
     2881 cgttattgat aaataacgaa gcggcacaag gtgtacagcc tacgttcata ctccgtaatc
     2941 aagttgcgga tgttgcgggg aagccacgta acgcgtttgt ttttatacgg cgcgatgaga
     3001 aatcaataca atcgaaaaaa atatttaaat catagtggat acgcgtatga cattggtttt
     3061 acgatcagga aaatggattg cactggtgct tttggccgga ttggtttccc aaacggcgct
     3121 ttacgcacaa cgcggcaccg ggaacaagtc aaacaaagac acggtagccc tcgaaagcgt
     3181 ttccagcgag gatctgttga agctcaagga agaacttgag tcgcaacgca ataagttgga
     3241 aaccgaacgg gtccagttgc ttgataaagg catcaaacag agcaaagagt ttcttgataa
     3301 atcgggttcc ggcacggcca cgacagcgct cgtacttttg cagcgcgcgg agtacctcta
     3361 tcagattatg aatgataact tttccgttgt ttccgattcg atagcgacgg aaaatcaaaa
     3421 aattctcaat gaatacgaac gtcgtaaaga ggattttcgc accaatcttg tcgcgcagaa
     3481 aaaaacggat aaagaaatag aacaagagat gaatcaatat ccggggccgc aacttttgcc
     3541 ggatccggag atggattata cggaagtcat caaaacatac cagcagttaa tggataattt
     3601 cccggaaagc ccgtatgtcg ttgatgcgat gtataatatc gcttttttcc gtgaggcgga
     3661 aggccgtcaa ctcaagtctc gcggcgatga acgctttatc aatgatggcg accggagaca
     3721 acgcgaagca ttgaaaattt atcaagattt ggctgttcgt tttcccgaca gtaaatacgc
     3781 aggtgaatgc tataaccgca tcggtgaata ttattttcaa cgcggcggcg atgccgattt
     3841 gcagaaggct atcaaaaatt atagcaaagt tttagattac ccccaagccg aacgctttca
     3901 ggaggctgtt tataagttag cttggacgca ttatcgtctc ggtgattatc ctaaagcaat
     3961 aagttatttc acatacctcg tagatgatgt ggattcggcg cgttattaca ataatttcag
     4021 ccaagaactt gatgtcgagg cgctcgttta tatcggtatc agttttaacc gctggggtga
     4081 acaaatagat ctcgcgcaag gtacggcgga tggcggatac aaattgatta agtcgtacat
     4141 cgatgaagct aaactcagtg aaaaacgcta cgccccggaa attatttggc aattgggcga
     4201 atcttataat cttgagcaaa aagatacctt agcgttgtat gcgtataaca cgttgattaa
     4261 tacctatcct ctattttggc gtacagcgga tgcgcagttt aaagtgatca acacctttga
     4321 acgtatgcag cgtggtacgc aaaataaagc ggttgccaag tcgcttttgg acagcgtgat
     4381 tttccatcgt tataagctgt ataattcata ccgccccggc agcgactggt ctgtagccat
     4441 gacggataaa gatttggtca ttcgggctaa tcgtatggcg cgcgatgtgt tggtggataa
     4501 tatattgtat tattacggcg aagcctcgtt gagcaatgat ctcaaagact ggcgtgtagc
     4561 gatggacttt agccgccagt ttatcacgtt tttccctgtt gatacatttg cgtataaatt
     4621 ccattacaat atggcattta tcgaatttgc attttttaaa atgctggata cggcgtatga
     4681 agactttgtc aaggtcgcga cattgtaccc atatgatcaa tatcgtttca agtcggctat
     4741 gaatgcgtac gtgattgccg actcgttgta tcgcgcccgt cctttcgcac gtcccaaaga
     4801 cgtgccgatg gacagtatat tgcctattac accaagcgaa tccaaactgc ttgatgcgat
     4861 caacaattac gcacgtcttt tcccggatac gtccgcattg tatccgaccg attcattgga
     4921 aaatccgaaa ccggtaatga cgaaaccggg ttataaaaca cccgacttct tagcttacgc
     4981 cggcggcatt tacttctcgc ataatgactt tgcgcgggca tcgcagtatt ttaatacgat
     5041 tgtaacacgg tatccaaaga gcgataaagc gcgtttggcg caacgttacc tgatgcaaac
     5101 ttatcgggat aaaaaagatt tccgtagttc tgaaatcgtg gcgcgtaaac tgttggaaaa
     5161 ttctgaagta agcgccgaac aaaagacgga agcggttcaa gttatatttt tctccatttt
     5221 caagcacgcc gaatactttc aaggtaaaaa agaaaattat aaagccgcac gtgagtttca
     5281 gcgcgcctac gaagaaggta aaaaacttgg ttatggtaaa cgtaatgaac tggctatagc
     5341 gatgttcaac tccggcgtgg agtactataa ttcgaaagaa ttgaaacgtt cgatagccgt
     5401 atttgaagcc tacgctgaca cgtttaaaga tactaaagaa gcgccggcgg ctttatggaa
     5461 cgtacagaat atgtattcgg atatgaagga aatgaaaacg gcggcacaag tcggtgagcg
     5521 tctcgttgac cgctatccta actattcgga cggttcgcgc tcttcagaag tagcgctata
     5581 taattctgaa tatttctttg aacagtcatc taaacagtcg gcggctaaag gcgacagcaa
     5641 cgaagctaaa gatctgaatc gtcaagcgat ccgggttagt gaaaaatttg tcaagctttt
     5701 ccccaaatcg caatacacac cagaactgga ttttaatatt gcgaaattat actt
//
LOCUS       DAOQNQ010000209         5750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_5557_length_5750_cov_5.938894, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000209 DAOQNQ010000000
VERSION     DAOQNQ010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5750
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5557_length_5750_cov_5.938894"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /gene="mutL"
                     /locus_tag="PK754_11670"
     CDS             complement(<1..1015)
                     /gene="mutL"
                     /locus_tag="PK754_11670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNB10208.1"
                     /translation="MENRIQILPDHIANKIAAGEVVERPASVVKELVENSLDAGADEV
                     TITVRSGGKDLVQVIDNGKGMTQDDAVLCFERHATSKIRQWEDLLKVQSFGFRGEALA
                     SIASVSDIELKTRMPDQEVGTLVQLHAGKLNNVTQEASLPGTTITVKNLFYNVPARRN
                     FLKTNTTEFRHIAGVIKRFAIAYPAVEFTLIHDDEKIFQLRKTDLYGRLEKVIGREIM
                     SYTLPLMADQFGVKIHGYVSKPSFSYKTKGEQFLFVNGRPIVNRTANFSVASAYGHTI
                     PKGDKPFFVLFLEWNSEEFDVNVHPTKAEVKFKDEQLLYRVVFHAVRDTMNTDVMIPS
                     LTQS"
     gene            complement(1017..1943)
                     /locus_tag="PK754_11675"
     CDS             complement(1017..1943)
                     /locus_tag="PK754_11675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB10209.1"
                     /translation="MSGSLRFETVLCPVCSHDQYSPWMQFNDKYAEHTLVRCTQCRFV
                     YINPRPTEDTIGVYYSAEEYTPFVSSSDNRSLFAKVYHTVRRFSVRWKRKKVENKSNK
                     GLVIDLGCGTAEFLNEMKQNGWQVAGIEPSPEASEFARSNYDIPVLTGTINRELIGQT
                     PDNPDVITMWHVLEHVHRLDETLDLIYKKLKPGGTLVIAVPNINSLDALFYGRDWVAL
                     DVPRHLYDFVLADLRSLLNKHQFRVYRTHGMPIDAVFNVLMSEKNIIQRRGLWFLPLS
                     LVRIPLVVSITTMAGLIRGRASAIVVYAEKQG"
     gene            complement(1950..2318)
                     /locus_tag="PK754_11680"
     CDS             complement(1950..2318)
                     /locus_tag="PK754_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10210.1"
                     /translation="MQRIFGFLIFSFLMAGCGNTSRLIVYTDANYAMVEKGKNIRVKH
                     VDGKFVQFYKMIIDKTDDEYIYARCWKTKKSTPEDLRFKKSEVILESTEFSVSRTTEY
                     AMLIGVTILLIFLSDRILRG"
     gene            complement(2329..2601)
                     /locus_tag="PK754_11685"
     CDS             complement(2329..2601)
                     /locus_tag="PK754_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10211.1"
                     /translation="MQQSGLMLKIGGWFSAVLLSVGLLIRLSGNLALSHFVLISGIVV
                     MLISPLAGMLVMIVKFWKNGEIRFAYIGVLILFLLAGGTYILHILQ"
     gene            complement(2603..3436)
                     /locus_tag="PK754_11690"
     CDS             complement(2603..3436)
                     /locus_tag="PK754_11690"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNB10212.1"
                     /translation="MEILAYLIIGFTAGAVGSMVGLGGAIIMTPALTILLNVPIKSAI
                     GASLIALIATSVMSTAVYSKKELIHYRYGMLLLILMVSGSFIGSAIGVMMDARLLSLF
                     FVALMIFAAAFLLYQVFQNENPDARVEWQNDEEPHRFPPNGVLLEAHSGIRKFYRVQH
                     GVVMMFISGIAGVISGMLGVGGGIIQVPFMHAINRMPIKAATATSGFMIGYTGMAGAL
                     VYVIFGQFELIMTSALVLGILTGSQAGARLAIRLHARWIIYLVIVVLTVAAIRLFMKV
                     F"
     gene            complement(3444..5192)
                     /locus_tag="PK754_11695"
     CDS             complement(3444..5192)
                     /locus_tag="PK754_11695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNB10213.1"
                     /translation="MKFTFNRVKTLIVVSALSVTFIGGWFAESLIGDDYYYEVKRSLR
                     LFGDVYKNVGEKYVDEISPKVIMRTGIDAMLETLDPYTMFYEGSESSDLDAFMKGKYG
                     GIGVTVGIRRNTIMVITVTEGSPAYKAGLKPGDRIVGVDGKTTRDIPIRDLSNSVKGE
                     PGTSLKLDVKREGQSSALQMTMIREMIAIENIPYYGVLAGDIGIIKLTKFSKTSTSDF
                     TRALKDLRSQGIKGLIIDLRGNPGGLLDAAIDITNQFVRKGELITYTKGRIQDANRSY
                     YANNDPLIPQLPVAVLVDGNSASASEIVSGAMQDLDRGILVGSTTFGKGLVQTVYPLQ
                     ERDAVLKITTAKYYTPSGRCIQREHYAHDRSNAMRLESEDEPNEFYYDVFGEEDQVRD
                     SLTNDSLPSKRQEFKTKTGRIVYAEGGITPDIIKDDYSFLGYTREIVRRGYVFDFATR
                     FSAQNTRIDTGFVPDEELLTEFYKFLKDHHFNYNSTADSQLTALRAYAENNGYSAQIQ
                     QSILQLQNKFDEEEVKEFEAAKTAIRNLLQQEIISRYYGNRRRFEITLKSDPVVESAR
                     SVLKDEVLYKKILKQK"
     gene            complement(5203..>5750)
                     /locus_tag="PK754_11700"
     CDS             complement(5203..>5750)
                     /locus_tag="PK754_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10214.1"
                     /translation="LRVDGTNLPPEPIFENDTTILQKLPNGLHVSSDNIYEPEHIVIV
                     PEPEIPGTQWDHRGYHHGYRYMWITRGKPTVEAVWKPKLSKSGSYKIQVHIPKKFADG
                     VAFYRVSTKNGIDTVKVNQSKYTDEWITLGVYELDKNDEISVMLNNSTPQGKGSVAFD
                     DVRFVGQWDTYSKKGFNKPIQ"
BASE COUNT         1640 a         1348 c         1129 g         1633 t
ORIGIN      
        1 gagattgggt caggctggga atcatcacat ccgtattcat agtgtccctt acggcatgaa
       61 atacaacacg gtacaaaagc tgttcatctt taaatttgac ctcggctttt gtcggatgca
      121 cgttgacatc aaactcttct gaattccact ccagaaataa aacgaaaaac ggtttatcac
      181 ctttgggaat ggtatggcca tacgccgacg ctacagaaaa attcgccgtg cggttcacga
      241 taggccttcc attgacaaaa agaaattgtt cacctttggt tttatatgag aacgagggtt
      301 tacttacata tccatgaatt tttacgccaa actgatccgc cattagcggc aatgtgtatg
      361 acatgatttc ccgtccgatg actttttcca ggcgaccgta aagatccgtt ttccgcaatt
      421 ggaatatttt ttcatcatca tggatcaagg taaattcaac ggcgggatat gcgattgcaa
      481 atcgtttgat gacacccgca atatgccgaa actcagtcgt attggtcttt aaaaaattac
      541 ggcgcgccgg tacattgtag aacaaatttt tgacggttat cgtagtaccc ggcaacgatg
      601 cttcttgcgt tacattgttc aactttccgg catgcaattg gactagggtt ccgacctcct
      661 gatcgggcat acgtgttttt agttcgatgt cggagacgga tgcaatactg gccaaggctt
      721 cccctcgaaa accgaaactc tgtactttga gcaaatcttc ccattggcgt attttgctgg
      781 ttgcgtgcct ttcaaaacaa agcacagcat cgtcttgcgt cataccttta ccgttatcaa
      841 tcacctgcac aagatctttt cctccggatc ggaccgtgat cgtaacctca tccgcaccgg
      901 catccaaact attttcaacc aattccttga ctaccgaagc cgggcgttca acgacctcac
      961 cggccgcaat tttattggca atatgatccg gtagtatttg tattcgattt tccatgttat
     1021 ccttgttttt cagcataaac tacgatagcc gatgctctcc cgcgtatcaa cccggccatc
     1081 gtagtgatag acactaccaa tggaatgcgt accaatgaca gagggagaaa ccacaaaccg
     1141 cgtcgttgaa taatattttt ttcactcatc aatacgttaa ataccgcatc aataggcatg
     1201 ccatgcgtgc gatatacccg aaactgatgt ttatttaaca acgatcgaag atcagctaaa
     1261 acaaaatcgt acaaatgacg cggtacatcc aatgccaccc aatcgcgtcc gtaaaaaaga
     1321 gcatctaatg aattaatatt aggtacagcg ataaccaaag tgccgcccgg tttaagcttt
     1381 ttataaatca aatcgagtgt ttcatccaaa cgatgcacgt gctccaatac atgccacatg
     1441 gtaataacat cagggttgtc cggtgtttgc cctatgagct cccgattaat agtacctgtg
     1501 agaacaggaa tatcatagtt tgagcgtgca aattctgatg cttccggcga aggttcgata
     1561 ccggcaacct gccagccgtt ttgtttcatt tcattgagaa actcggccgt accacatcct
     1621 aaatcaatga ctaaaccttt gttcgatttg ttttctactt ttttacgctt ccatcgtacg
     1681 gaaaaacggc ggaccgtatg ataaactttg gcaaataaac tgcggttatc tgacgaactt
     1741 acaaacggcg tgtattcttc ggcggaataa tagaccccga tggtatcttc tgtcggtctt
     1801 gggttgatgt atacaaaccg gcactgcgtg caccgaacca gcgtatgttc ggcgtattta
     1861 tcattaaatt gcatccatgg tgaatattga tcatgcgaac ataccggaca cagcacggtt
     1921 tcaaaacgta aggaacccga cattatgcat taccctctca aaatacgatc tgaaagaaat
     1981 attaataaaa ttgttacacc gatgagcatg gcgtattccg ttgtgcggga cacactaaac
     2041 tctgtacttt ccaatatgac ttccgatttc ttaaaacgta aatcctccgg cgtacttttt
     2101 ttagttttcc agcaacgggc gtaaatatat tcatcatctg ttttatcaat tatcatcttg
     2161 taaaactgaa caaatttacc gtccacgtgt ttgacacgta tgttttttcc tttttcgacc
     2221 atagcgtaat tagcatcggt ataaacgatc agtcggctgg tattgccgca tccggccatt
     2281 aaaaaactaa aaattaagaa tccaaatata cgctgcatag ataccccttt actgtagaat
     2341 atgaaggata taagttccac cggctaataa gaataatata agtaccccga tatatgcaaa
     2401 tcggatttca ccgtttttcc aaaatttgac aatcattacc agcataccgg ctaacggact
     2461 gatcaacata acgacaatgc cgctgatcaa aacaaaatga gataatgcta aattaccact
     2521 caatcgtatc agtaaaccga cggataaaag aaccgcgctg aaccaaccgc cgatttttaa
     2581 catcaaacct gattgctgca tttcaaaata ctttcatgaa aagtcgaatt gccgcaacgg
     2641 tcaagacaac aataactaaa tatatgatcc atcgtgcatg cagacgaatg gctaaacgtg
     2701 ccccggcttg agaaccggtg agaataccca gaacaagtgc cgaagtcata atcaattcaa
     2761 attgcccaaa aataacatag accagagccc cggccatacc cgtataacca atcataaatc
     2821 ctgatgttgc tgttgccgcc ttgatgggca tacgattgat cgcatgcata aaaggtacct
     2881 gaataatacc tccaccgact cctaacatac ccgaaatcac ccctgcaata cccgaaataa
     2941 acatcataac gacgccatgc tgcacccggt agaatttgcg aataccgcta tgagcttcca
     3001 ataatacgcc gttgggagga aaacgatgcg gttcttcatc attttgccat tcaacccggg
     3061 catccgggtt ttcattttga aaaacctgat acagcaaaaa agctgcagca aaaatcatca
     3121 acgctacaaa aaaaagagaa agcaaacggg catccatcat tacgccgatt gcgcttccta
     3181 taaaagatcc ggacaccata agtatcaaaa gaagcatacc gtaacgataa tgaatcaatt
     3241 cttttttaga ataaaccgcc gttgacatga ccgaagtagc aatcagcgca ataaggctgg
     3301 cgccgatcgc ggatttgatc ggtacattta gcaggatcgt taaggccggt gtcatgatga
     3361 tcgctccgcc taaacctacc atcgaaccga cggctccggc tgtaaagcca atgattaaat
     3421 atgcaagaat ttccaatgga tagttatttt tgttttaaaa tctttttata caacacttca
     3481 tctttgagca cgctgcgagc ggattcgact accggatctg atttgagcgt tatttcgaaa
     3541 cgacgtcggt ttccataata tctggaaatg atttcttgct gcaaaagatt tcgtatcgcc
     3601 gttttagcgg cctcgaactc tttcacttct tcttcatcaa atttgttctg aagttgtaga
     3661 atggattgct gtatctgtgc cgaataacca ttgttttcgg cgtaagcgcg taatgcagtc
     3721 aactgcgaat cggcggttga attataattg aaatgatgat ctttgagaaa tttataaaat
     3781 tcggttaaca gttcttcatc cggtacaaat cccgtatcaa ttcgggtatt ttgagccgaa
     3841 aaacgagttg caaaatcgaa tacgtacccg cgacgtacta tttctcgtgt atatcccaaa
     3901 aaactataat catctttgat gatatccggt gtgatgccgc cttcggcata tacgatacgt
     3961 cctgttttag ttttgaattc ctggcgtttc gacggcaacg aatcattggt caggctatcc
     4021 cgtacttgat cctcttcccc aaacacatcg taataaaatt cattcggttc atcttccgat
     4081 tccaaacgca ttgcgtttga gcgatcatgg gcatagtgtt cgcgctggat acaacgtccg
     4141 gacggcgtat agtatttagc cgtagtaatt ttgagaaccg catcacgctc ttgcaatgga
     4201 tatacggttt gcacaaggcc tttaccgaaa gttgtgctac ctactaaaat accgcgatcc
     4261 aaatcctgca ttgccccgct cacgatttcc gacgcactgg cgctattacc gtcaactaag
     4321 acggctaccg gcaattgtgg aatcaaaggg tcattattgg catagtatga acgattggca
     4381 tcttggattc gaccttttgt gtatgtgatc aattcgcctt tacgaacaaa ttgattggta
     4441 atatcaatgg ccgcatcaag aagtcctccc ggattcccac gcagatctat gattaacccc
     4501 ttgatgcctt gtgatcttaa atcttttaaa gcgcgcgtga aatcggatgt agatgtttta
     4561 gagaacttag taagcttgat aatcccgata tctccggcca atacgccgta atagggtata
     4621 ttttctatgg cgatcatctc acgaatcata gtcatttgca aggcgctgct ttgaccttcg
     4681 cgtttaacat caagtttcaa tgatgttccg ggttcgcctt ttacggaatt agaaagatca
     4741 cgtatcggta tatcacgcgt cgttttacca tccacgccga cgatacgatc tcccggtttt
     4801 aaaccggctt tgtatgcggg tgatccttct gtcacggtga ttaccatgat cgtattgcgg
     4861 cgaatgccga cggtcacgcc gatgccgccg tatttgccct tcataaaagc atccaaatca
     4921 ctgctctcag aaccttcata aaacatggta tagggatcga gcgtttcaag catggcgtca
     4981 atacccgtac gcatgattac tttgggcgat atttcatcta catatttttc accgacattt
     5041 ttgtacacat cgccaaacag cctgagcgaa cgtttgactt cgtaataata atcatcaccg
     5101 ataagcgact cggcaaacca gccgccgatg aatgtaacac tcagtgcgct tactacgatc
     5161 agagttttca cccgattaaa tgtaaacttc acaaaaggcc tttcattgaa ttggtttatt
     5221 aaaacctttt ttactgtagg tgtcccattg acctacaaat cgaacatcgt cgaatgcaac
     5281 ggaacctttt ccctgaggtg ttgaattatt aagcataacc gatatttcat catttttatc
     5341 aagttcatac acgcccaaag ttatccattc gtcagtatat ttgctttgat ttactttgac
     5401 cgtatcaata ccgtttttag tgcttacacg atagaaggcc acgccatcgg caaatttttt
     5461 ggggatgtgt acttggattt tataggaacc cgacttagaa agtttcggtt tccatacggc
     5521 ttccaccgtc ggtttacctc tcgtaatcca catataccgg tatccgtgat gatagccgcg
     5581 atgatcccat tgtgtaccgg gtatctccgg ttcaggaacg atcacgatgt gctcaggttc
     5641 atatatatta tcggatgaaa catgaaggcc gttgggtaat ttctgcaaaa tggtcgtatc
     5701 gttttcgaat atcggttccg gtggcagatt ggtgccatcc accctcaact
//
LOCUS       DAOQNQ010000210         5733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_5579_length_5733_cov_5.422860, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000210 DAOQNQ010000000
VERSION     DAOQNQ010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5733
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5579_length_5733_cov_5.422860"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PK754_11705"
     CDS             <1..863
                     /locus_tag="PK754_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10215.1"
                     /translation="HKLRVLYLSGNPISNSELLKLKGVLPNTDVVYIPVDYFSNAIPS
                     PLTDADEEAISALKAKPAQLFKEDEYGLSSYSEIGRIFEEYGDFERAFRAYYMGMSMG
                     SGGDAYALGNLIELHSNLKEHKDKVSAALPYFNKACSLGFEDGCTAAKNSREHLQSVT
                     KHEERMRQMWKDFSLEATSTLSQIAVTVVTKGTNQQTINQSAASAGNEGSTDCSTCDN
                     DPTYLRLKKEAESCLSCSYCAAVRLLQCYIEKGCYKEAGANTSVTLQELKRRIEENRN
                     NAKKMGNICN"
     gene            1780..3129
                     /locus_tag="PK754_11710"
     CDS             1780..3129
                     /locus_tag="PK754_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10216.1"
                     /translation="MRSNLMRFAGIVALPLVFLAFLFINSCEGPRGPAGADGKDGTNG
                     TNGTDANETCKTCHNPNDVDRIKIEFQFSKHEYGEAAFEEAGNTSCAVCHTQQGFLYV
                     VQNNVPSTFTLNAGTGKYSNNYATISSQALGEIGCGTCHASTHTTYGAADTVLTTNAA
                     VAMTMWKGNKTINLAQDNGRSNLCIKCHQPRPMTKSTTLSNGDIVNYDSLALDPNRMF
                     YDSTTGASNTHTYPSYRMHVHYGVVGAVFAGMGGVEFTGSKTVQSSTHTSVASCQDCH
                     MATVSGRGGGHTFMVQGNFNGCNTSGCHTGVSSSNSTLWTNPRNTIKGLLNTLATKIN
                     AAGGGTPILHADSDTETNLWAGLTTGNWDGYLNIYDPSSNPGGAWRNPNPSGSWTQAQ
                     KDANLALPKFPSLKYVQVGAMINFQFALREASLGIHNYKYSEALLTNTIEKMTDAGF"
     gene            3474..4847
                     /locus_tag="PK754_11715"
     CDS             3474..4847
                     /locus_tag="PK754_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10217.1"
                     /translation="MNIKKRLVGYLIVLCGAVVLFDASAQTLSTRLSTSVYSWENQYA
                     DSTTQKSFRGYQTAIFHANGIWKSNISLHGYVTGSYDLAGARQDNPDYRVYNLYAQWK
                     SDPTKNHRWEARVGRQQVYAGVRAPSIDGVRYDFYNADKYGFMAYYGALPPADGTVKM
                     GRPADRSAFGAKVMTSKLAQTHMALTYVERRSIGQYYYTINGRNADTLVYSPDLDERL
                     IAFDANRNFFGKINWFYNVTYDALASNVRKISTDVQFQPLHTLWFAVQYAYRRPDLYY
                     NTIFAKFDDIEANQELWVRGTYQFNTDWSFNGEYANVFYKMKDAWRYGAGLTYQRTNV
                     SYQWRLGYGGTIKQISLSSYYPVSRSMYLYGGLTYATYDMKDDITSSDFLSSSTQDIY
                     RQVYQYTNSSMTAVLRCNYNLMQAMNVDVEGQYLSQNIKTSPVFAGSKYDLRFFVRAN
                     YWFFNKI"
     gene            4866..>5733
                     /locus_tag="PK754_11720"
     CDS             4866..>5733
                     /locus_tag="PK754_11720"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNB10218.1"
                     /translation="MKKLLGIILITAVGLMAAALVFFPSSEPLKASSLVKTSAQMQDK
                     LGSRYAIIYKEEDRSKLIKHSHKFHATKGGAECKDCHASITSSEKAMDSNLPKMDACY
                     ECHDKETTDCKLCHLEPKEPYSSYKSPKRELTFNHKAHLGMKNVNCETCHSEITNKAY
                     TGARTLPAMESCMGCHDGAQASNDCKTCHTDIRFIKPADHMAGFMRTHKHEVATQGDA
                     NCRMCHSTESCEDCHNGGNLNKMKNRNEFSPSGTPSGMSENSSQIISKAHALDFIFTH
                     RFDAKAKTSECQT"
BASE COUNT         1673 a         1228 c         1309 g         1523 t
ORIGIN      
        1 tacataagtt gcgagtactg tatctcagtg gcaaccccat ctctaacagt gaattgctca
       61 agttgaaggg ggtgcttcca aatactgatg tcgtttacat tcctgttgac tatttcagca
      121 acgcaattcc ttctccgcta accgatgcgg atgaggaggc tataagcgcg ctcaaagcaa
      181 agcccgcaca gctttttaag gaagatgagt atggtctatc atcttattcg gaaataggta
      241 ggatatttga agaatacggc gactttgaaa gagcatttcg ggcatactac atgggaatga
      301 gtatgggtag cggaggtgat gcttacgcct tgggtaactt aattgagttg cattcaaatc
      361 tgaaggaaca taaagataag gtctccgccg cccttcctta cttcaacaag gcttgttctc
      421 tcggctttga agatggatgc actgcggcca aaaacagcag agagcatctc caatccgtga
      481 caaaacacga agagcgaatg aggcagatgt ggaaagactt cagtcttgaa gctacctcca
      541 cgctctccca aattgcagta acagtagtca caaaaggaac taatcagcaa acgattaacc
      601 aatcagccgc atctgctgga aatgaaggat cgaccgactg ttcgacctgc gacaatgatc
      661 cgacctatct taggctgaaa aaggaagcgg agtcctgctt gtcgtgttcc tattgcgccg
      721 ccgtcagatt gcttcaatgc tatatcgaga aaggatgcta caaggaagca ggtgccaata
      781 cttcagtaac tctacaggaa cttaaacgcc gaatcgagga aaatcgaaac aatgcaaaaa
      841 aaatgggtaa catctgtaat tagtgtggct gttctttttg aattatgttt ctttagccaa
      901 gaacttgcgg ctcaaccaga ttccgcccgg tatcaaactt ttaggtttgg aaataagaga
      961 agggatttca ttacgagacc atgacatatt atccgaagaa taggattctc atttcggtaa
     1021 attgtgaaaa gaatgagacg ttggattgcg atgcttatcg tcttctcctc agagcaacat
     1081 gccagggcgt cgattccaca cgatggattg gcaattctgg cgcggtggta tgtaatttta
     1141 tgggaggcac caacgtgacg ggggaagatt ctcttcgaaa tcaatacggc ttttgtaagt
     1201 gcgaagacgg atcaatggtc tctacaagcg cgttgtatgg ttttgcctta gataatgatg
     1261 aaaggaatca aaaaaaacaa agccaaggcc agagcttgaa agatgtaaat aaatagagga
     1321 gaatctatga aattactttt cacaattttt accgcggcag ttctctccgc aactttcctg
     1381 cacgcgtagc aaggctagaa aaaaaacaca gtatggatat gcgttcatct atagtagagc
     1441 aggcatttat acaaggccgg ggtataaatc ttggtaataa acaaaccgat ctgtatcaac
     1501 ggtttaagaa aaagtatgat caaaactaag tagcttgtta acaagtgatc attttaatca
     1561 ttgaaagcgg gccaaagccc gcttttttta taattatttt ttatgatttg taataattta
     1621 gtcaaaaaat gagttttaaa atattgttaa aactttgttt atgagcgaaa attttttatg
     1681 acaaatttat gacaattatc atagggatgg tatggacgtg tgtttatttt atatgcgaga
     1741 ttcaactcca aaccaatcct aagtaggagg taaaaagcga tgagaagtaa cttgatgaga
     1801 tttgcaggca tcgtcgcact accattagta tttttagcgt ttttgtttat caatagctgc
     1861 gaaggcccga gaggccccgc cggagcggat ggcaaagatg gtacaaatgg cacaaatggt
     1921 actgatgcaa acgaaacatg caaaacatgc cacaatccca atgatgtgga tcgtatcaaa
     1981 attgaattcc agttttccaa acatgaatac ggcgaagccg cttttgaaga agccggcaat
     2041 acatcttgcg ccgtatgcca cacgcaacaa ggattcctgt atgttgttca gaacaatgtt
     2101 ccgtcaacat ttacgttgaa tgcaggtacc ggcaagtatt ccaataacta tgctaccatt
     2161 tcttcccagg ctttgggtga aatcggttgc ggtacatgcc atgccagcac tcatacgacg
     2221 tatggtgctg cggataccgt attaacgaca aatgcggccg tcgcgatgac gatgtggaaa
     2281 ggcaataaga cgatcaattt ggctcaagac aatggtcgtt ctaatctttg catcaagtgc
     2341 catcaaccgc gtcctatgac aaagagcacg acgctcagta atggcgatat cgtcaattat
     2401 gattctttag cattagatcc gaatcggatg ttctatgatt caacgacggg tgcttccaat
     2461 acgcatacct atcccagcta tcgcatgcat gtgcactacg gtgtagtagg tgctgtattt
     2521 gcaggtatgg gtggtgtaga attcacagga tctaaaacag ttcaaagttc aacgcacact
     2581 tccgttgcgt cctgccaaga ttgtcacatg gcgacggtca gtggtcgtgg tggcggacac
     2641 acatttatgg tgcagggtaa ctttaatggt tgcaatacct ccggttgcca taccggcgta
     2701 agttccagca actctacctt gtggacaaat cctcggaaca cgatcaaagg tttgctcaat
     2761 acattggcta cgaagatcaa tgcggccggc ggcggaacac cgatcctcca cgctgacagt
     2821 gatactgaaa ccaatttgtg ggcaggtttg acgacaggca attgggatgg atatctgaat
     2881 atatatgatc cttcatccaa tcccggcggg gcatggagaa atcccaatcc aagcggctca
     2941 tggacacaag ctcaaaaaga tgcaaactta gcattaccga agttcccatc gctgaagtat
     3001 gtacaagtcg gtgcgatgat taacttccag tttgcactga gagaagccag cctcggtatt
     3061 cacaactaca aatattcgga agctctgctg acgaatacga ttgaaaaaat gacggatgca
     3121 ggcttttaat tagtacgatc gttacgattc ttcaaaggtc gctcgctaaa acgagcgacc
     3181 ttatttttta tctattttcc gctttgttcc acaaatttta agcaaaaacc ttctgatgct
     3241 taaaatcaaa cgacccataa gttgttaaaa cgctggttac atcagtttgt tgactgatga
     3301 taattaatgc aaagtaatgc tataattttc tttacaattt tatgacaatt gtcatagggt
     3361 gtattgcgac ttgggcatat cttttgatga aataagtgcc tttctgtatt tttattagct
     3421 ttattttgac atataattta cgacgattca tggacttgaa gcctgaggtt gctatgaata
     3481 tcaaaaaaag attagttggc tacttgatag tgttgtgcgg cgcagtcgta ctttttgacg
     3541 catcagccca aacgctttca acgcggcttt cgacatcggt gtattcctgg gaaaatcagt
     3601 atgcggattc aaccacacaa aaaagttttc gtggttatca aacggcgatt tttcatgcca
     3661 acgggatttg gaaatccaat atttcgttgc acggatatgt aaccggaagt tatgatcttg
     3721 ccggtgcgcg gcaggacaat ccggattatc gcgtgtacaa tctttatgcg cagtggaaat
     3781 ccgatccgac aaaaaatcat cgttgggaag cccgcgtagg acgccagcag gtttatgccg
     3841 gtgtacgtgc gccttcgata gatggtgtcc gttacgactt ttataacgcc gacaaatacg
     3901 gatttatggc ttactacggt gcactgcctc cggccgatgg aactgtaaaa atggggcgcc
     3961 cggcggatcg ttccgcgttt ggtgccaagg tgatgacttc caagttggcg caaactcata
     4021 tggccctaac gtatgttgaa cgacgcagca ttggtcaata ttactatacg atcaatgggc
     4081 gcaacgcgga taccctcgtt tacagtccgg atttggatga gcggctcatc gcttttgatg
     4141 ccaatcgcaa ttttttcgga aagatcaatt ggttttataa tgtgacgtac gatgcgttgg
     4201 cctctaacgt gcgtaagata tctacggacg tgcaatttca accgttgcat acgctgtggt
     4261 ttgccgtaca gtatgcttac cgccgaccgg atctttatta caataccatt tttgcgaagt
     4321 ttgatgacat tgaggccaat caggaactgt gggtgcgcgg aacgtatcaa ttcaataccg
     4381 attggtcgtt taacggcgaa tacgccaacg tattttacaa aatgaaagat gcttggcgtt
     4441 acggcgcggg cctcacgtac caacgcacaa acgtatctta tcaatggcgt ttggggtatg
     4501 gcggcacgat caaacaaatt tcgttaagct cgtattatcc tgtcagccgg agcatgtatt
     4561 tgtacggcgg cctcacttat gcaacatatg acatgaaaga tgacatcaca tcgagcgatt
     4621 ttttgtcaag cagcacacag gatatctacc gtcaggtata tcaatatacc aatagttcca
     4681 tgacggccgt tctccgttgc aattataatc tgatgcaggc gatgaacgtg gatgtcgaag
     4741 gacaatacct ttcgcaaaac atcaaaacat cgccggtttt tgccggatcg aaatatgacc
     4801 tgcgcttttt tgtgcgcgcc aattattggt tcttcaataa aatataactt ataacggaca
     4861 ctggtatgaa gaaactactc ggaataatac tcattacggc ggtcggactc atggccgctg
     4921 ccttggtttt ctttccttct tcggagccgc ttaaagcttc gtcgctggtg aaaacttccg
     4981 ctcagatgca agataagctc ggcagccgtt atgcgattat atacaaagaa gaggatcgct
     5041 ccaagctgat caaacacagt cacaagtttc acgccactaa aggcggcgcc gaatgtaaag
     5101 actgccatgc ttcgatcact tccagtgaaa aagcgatgga cagcaatctt cccaaaatgg
     5161 atgcctgcta cgaatgccac gacaaagaaa cgacggactg taagctctgc catcttgagc
     5221 ccaaagaacc gtattcatcc tacaaatcgc ccaaacggga attgacattt aatcacaagg
     5281 cccatctcgg gatgaaaaat gtaaattgcg aaacctgcca cagcgaaata accaacaaag
     5341 catataccgg cgcacgtaca ctgcctgcga tggagtcttg tatgggttgc catgacggcg
     5401 cacaggcgag caatgattgt aaaacttgcc atacggacat ccgttttatc aagcctgcgg
     5461 atcatatggc cggatttatg cgtacgcata aacacgaagt ggctacgcaa ggcgatgcca
     5521 actgccgtat gtgccatagc accgaatcct gcgaagattg ccataatggc ggtaatctga
     5581 ataaaatgaa aaatagaaac gaattcagtc cgtccggtac accgtcgggt atgagtgaaa
     5641 atagcagtca gattatcagt aaagcccatg cactggattt cattttcaca caccgttttg
     5701 atgccaaggc taaaacttcc gaatgtcaga cct
//
LOCUS       DAOQNQ010000211         5672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_5666_length_5672_cov_5.028307, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000211 DAOQNQ010000000
VERSION     DAOQNQ010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5666_length_5672_cov_5.028307"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PK754_11725"
     CDS             complement(<1..447)
                     /locus_tag="PK754_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB10219.1"
                     /translation="MIRIAMIEDDAEIRQNVTAFLKKDNRFTITASAGSVEEGLEAFK
                     THVPDILILDIGLPGMSGLSAIRLFREKFPDTDIVMFTVHDDPARIFEALCSGASGYL
                     IKTTPFADIARSLNELHNGGAPMSPQIARKVIQHFYPAPSQKPQSTL"
     gene            complement(523..1992)
                     /locus_tag="PK754_11730"
     CDS             complement(523..1992)
                     /locus_tag="PK754_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNB10220.1"
                     /translation="MKLEAYFQKFRTGTIGHDLTVQTPYGTQHIVYADWVASGRLYRP
                     IEEQLLSRFGPYVANTHTETTTTGTTMTHAYHEAKAIIKGHVNAAPNDVLIFAGSGMT
                     GAVAKFQRILNLRIPEKFSGRISVPEAERPVIFVTHMEHHSNHTSWLETIADVELVGR
                     DEKGLVDLNDLSRLLERYKNRPMKIASVTACSNVTGIQTPYHDIAKIMHQNGGYCFVD
                     FACSAPYVPINMHPADTDARLDAIYFSPHKFLGGPGTPGVLIFNAQLYGNKIPDQPGG
                     GTVTWTNPWGEHKYFQDIEIREDGGTPPFLQAIKAAMAIRLKETMDPSRMMAREEEML
                     PRVFQVLRQAPGLHILADHVEHRLGIISFYIDGLHYNLGVKLLNDRFGIQVRGGCSCA
                     GTYGHYLLNVTREFSKRITDEIDHGDMSEKPGWIRLSLHPVMTDQEVDFILRAISDVA
                     THHKTWSKDYVYDGSANEFRHKEFKENSAAITDWFRVEA"
     gene            complement(2105..2938)
                     /locus_tag="PK754_11735"
     CDS             complement(2105..2938)
                     /locus_tag="PK754_11735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB10221.1"
                     /translation="MDYLDYQYDPNDPALMAVTDELPFWSAPFGTELLNAIRFKPGMT
                     VLDIGCGSGFPMLEIAERIGKKGTVWGIDPWPAAVARLREKTERLGITQVHIIEGQAE
                     DATIGEHTCDLIVSNNGINNVSDIPKTLSTCRRSAKHGAQFVMTYNLEGTMRAFYDVL
                     RAALRDHRREENLPLIDQHIRQKRPPLDRMEAWIEESGFEIGEIKLRQFHMDFTDGKA
                     FFEHHLIRHWFLPSWKNLVPQEILRPVFTAMENRMNDIVADDDVFRIEIPFALIDATA
                     I"
     gene            complement(2968..>5672)
                     /locus_tag="PK754_11740"
     CDS             complement(2968..>5672)
                     /locus_tag="PK754_11740"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB10222.1"
                     /translation="YVWIGTLGGLSRWDGVRFQNFQTQDGLAGGQISALHEYHDGSMY
                     IGTDGGGLSVYRDGRFTTLNKDSGLCDNRVWNIIPSGDSALWICTYGGGVSFYQPQRR
                     SWKTIGLHEGLPDLRVYTGVHAGNDVWWFGTDSGVAILSPQTQQRITARDGLIHPIVR
                     SIRRLNDSAWALGTREGLSVYINGRCTNYTTRDGLADNDINAMLLLQNGALCIATANG
                     ISLWHHGVFENLTTANGLANDLVWNIYEDNDRTLYFGTNGGGVSMYRPGRLTTWRSGK
                     ELPDAPFTTVTQRRDGSMVFGSHAGLITMRGDKPDILDEKKGLAHNLVWSLFEDRRGR
                     LWIGTHNGIHTVTDGRVTATYHHSDKPMNRAYGMTEDADGNMILATRGGLAKIEDRRI
                     VPWATGTPLSGQIVWCARTLRDGRMAFGSDGAGLFIYDGSQFKNISQKNGLAGDFVVS
                     IYEAPDGLLCVGTDGGGLSLIRDGVIRNFDTKNGLRDNTIYAITGDTRGAIYAASNRG
                     MHILSFSGGDTLIRTMRRSDGLAGDECSLGGVCTDQNGRVWVATVNGMTEINPSSDSL
                     YTVPQTRIHRLRVYENSIPVVAHTAFRHDENYLKFDFIGIYPSAPEQVRYRYRLQGLD
                     AQWIPTTERTIQYTNLASGDYTFEVQSANDWGVWSTTESLVFTITPPFWKTWWFRLIL
                     VLAVIGLLWIIYRVRVTRLLAMERMRVRIASDLHDDIGSTLTRIAVSSESIRSSDDLS
                     AIRHSAERIGQLSRDVIRTFSDIVWSIDARNDRMGDLIARLQDTAYQVLNTAEIACTW
                     DVRGIRADESLAVDVRQNVFLIVKEVLHNAAKHSEATSMHVRIERTDRGLMISLRDNG
                     RGIPDERIGKGNGLRNMRMRADRVRGRIKQINDNGSVTEIFIPL"
BASE COUNT         1364 a         1483 c         1448 g         1377 t
ORIGIN      
        1 cagcgttgac tgaggttttt gcgaaggcgc gggataaaaa tgctgaatca ctttgcgggc
       61 tatctgcggc gacatcggtg caccgccgtt atgcaattcg ttcagtgatc gagcaatatc
      121 cgcaaacgga gtagttttga taagataacc ggatgcaccg ctgcataagg cttcaaaaat
      181 tcgcgccgga tcatcatgta ctgtgaacat cacgatgtcc gtatcgggaa atttttcccg
      241 aaagagacgt atcgcgctca atcccgacat accgggtaag cctatgtcga gaatcagaat
      301 atcgggcacg tgcgttttga acgcttccaa accttcttcg acagatccgg cggaagccgt
      361 tatcgtaaaa cggttgtctt ttttcaaaaa tgccgtcaca ttctggcgaa tttctgcgtc
      421 gtcctcaatc atcgcaatgc gtatcataaa cgatcctttg taaaacattc atcgcaaact
      481 aaggacgtca tcgcagaaaa ccaatcccat aacgatggga ggctaagctt cgacacgaaa
      541 ccagtcggtt atggccgcgc tgttttcttt gaactctttg tgacgaaatt cgttggccga
      601 tccgtcgtac acgtagtctt tcgaccatgt tttgtgatgg gtcgccacat cgctaatcgc
      661 gcgaaggatg aaatccacct cctgatcggt catcaccgga tgaagggaaa gccttatcca
      721 gcccggtttt tcggacatgt cgccgtgatc aatttcgtcc gtgatacgtt tggaaaattc
      781 gcgcgtcaca ttgagtaaat aatgtccgta cgtgccggca caggaacagc cgccgcgaac
      841 ttgaatgcca aagcgatcgt tgagcagttt gacgccgaga ttgtagtgca gaccgtctat
      901 gtaaaatgaa atgatgccga ggcggtgttc gacatgatcg gccagtatgt gcaaccccgg
      961 ggcctgacgc aaaacttgga atacacgcgg caacatttct tcttctctcg ccatcatgcg
     1021 cgacggatcc attgtttctt tgaggcgaat cgccatcgcg gctttgatcg cctgcaaaaa
     1081 aggcggcgta ccgccgtctt cgcgaatttc gatatcctga aaatatttat gttcgcccca
     1141 gggattggtc catgtcaccg taccgccgcc gggttgatcg ggaattttat taccgtaaag
     1201 ttgagcgttg aaaatcaaaa caccgggcgt gccgggtccg ccgagaaact tatgcggaga
     1261 gaaataaata gcatccaggc gcgcatcggt atcggcgggg tgcatattga tcggtacata
     1321 cggcgcggag catgcaaaat ccacaaaaca atacccgccg ttctgatgca tgattttagc
     1381 tatgtcgtga tagggcgttt gaatccccgt gacgttggaa catgcggtga cggaagcgat
     1441 tttcatcggc cggtttttgt agcgttccag taaccgtgaa agatcattca gatccaccaa
     1501 acctttttca tcacgaccaa ctaattctac atccgcgatg gtttccagcc atgaggtatg
     1561 attggagtga tgctccatgt gggttacaaa aatcacgggg cgttccgctt cgggtacgga
     1621 aatgcgtccg ctgaattttt caggaatgcg aagattgagt atgcgttgaa acttggcgac
     1681 cgcaccggtc atacccgaac ccgcaaaaat caaaacgtca ttgggagcgg cgtttacatg
     1741 ccctttgatg atcgctttag cttcgtggta cgcatgcgtc attgtcgttc cggtggtggt
     1801 ggtttccgtg tgcgtattgg cgacgtatgg gccgaaccgc gaaaggagtt gttcctcgat
     1861 agggcgatac agccgtccgc ttgccaccca gtcggcgtaa acgatgtgtt gtgttccgta
     1921 cggagtttgg accgtcaggt cgtgaccgat ggtgccggtg cgaaattttt ggaagtacgc
     1981 ttcaagtttc atgaatgcag agtgtataaa ggttaatgat ttttaaaccc gagaggataa
     2041 ttaaaaaaac aaccaactgc atgcaaaaaa tcatcggatt ttctgtaaat aaaatcgcgg
     2101 ttttttatat ggccgtcgca tcaatcaagg caaaaggtat ttcgatgcga aacacatcat
     2161 catccgcgac gatatcgttc atgcggtttt ccatcgccgt aaaaacaggg cgcaggattt
     2221 cctgaggcac aagatttttc catgaaggga gaaaccaatg gcggatcaaa tgatgttcga
     2281 agaaggcttt cccgtcggta aaatccatgt gaaactgccg cagtttgatt tctccgattt
     2341 caaatcccga ttcttcgatc catgcttcca tgcgatccag cggaggtcgt ttttgccgga
     2401 tatgctgatc aataagggga aggttttctt cgcggcgatg atcgcgcaac gctgcacgca
     2461 acacatcgta aaaagcgcgc atcgtacctt cgagattata agtcatgaca aattgcgcac
     2521 cgtgtttggc ggaacggcgg cacgtgctta aggttttagg aatatcgctg acattattaa
     2581 taccgttatt ggatacgatc agatcacacg tatgttctcc gatggttgca tcttcggcct
     2641 gaccttcgat gatatgcacc tgcgtgatgc ccaaacgttc ggttttttcg cggaggcgtg
     2701 cgaccgccgc cggccacgga tcaatgcccc ataccgttcc tttttttccg atacgttcgg
     2761 cgatttcaag catcgggaat ccgctgccgc aaccgatatc cagcacggtc atgccgggtt
     2821 taaagcgtat cgcgttgagc agttccgttc caaacggcgc cgaccaaaac ggcaattcat
     2881 cggtgaccgc catcagtgcg ggatcattgg gatcgtattg ataatctaaa taatccatac
     2941 ggtttttctt ttattagtga aattatttta cagaggtata aaaatttcgg tcaccgatcc
     3001 gttatcgttg atttgtttta tgcggccgcg cacacgatcg gcgcgcatgc gcatattgcg
     3061 aaggccgttg ccttttccga tccgctcgtc gggaatgcca cggccattgt cccgcagaga
     3121 aatcataaga ccgcgatctg tacgctcaat gcggacgtgc atggaggtgg cttccgaatg
     3181 tttggccgca ttatgcagca cttctttgac gatcagaaaa acattttgcc gcacatctac
     3241 agccagtgat tcgtccgcgc ggataccgcg tacatcccac gtgcaggcga tctcggccgt
     3301 gttgagcacc tgatacgccg tgtcctgcag gcgtgcgatc aagtcgccca tccgatcatt
     3361 gcgtgcgtca atggaccaga caatatcact gaaggtgcgt atcacatcac ggctgagttg
     3421 gccgatcctt tcggcggaat ggcgaatagc cgagagatca tcggaactgc gaatggattc
     3481 cgaactgacg gcaatacgcg tgagcgttga acctatatca tcatgcagat cgctggcaat
     3541 gcgcacgcgc atacgttcca tggcgagcaa tcgcgttacc cgaacacggt aaataatcca
     3601 caataatccg atcacggcca ggacgagtat taaccggaac caccaggttt tccaaaacgg
     3661 aggcgtgatc gtaaaaacca gcgattccgt cgtgctccat acgccccagt cgttggcgga
     3721 ctgcacctca aacgtataat cgcccgaagc gagattggta tattggattg tgcgttccgt
     3781 ggtaggaatc cactgcgcat caagaccttg tagccggtag cggtagcgca cttgttccgg
     3841 cgctgaagga tagattccga taaaatcaaa tttcaaataa ttttcatcat ggcggaaagc
     3901 cgtgtgcgcc accaccggaa tgctattttc atacacgcgc aaacgatgga tacgcgtctg
     3961 cggaacggta tagaggctat cggaactcgg attgatctct gtcatcccgt tgacggttgc
     4021 aacccatacc cggccgttct gatccgtaca cacacctccg aggctgcatt catcgccggc
     4081 gaggccgtcg ctgcgccgca tcgtacgtat cagcgtgtct ccgccggaaa acgaaagaat
     4141 gtgcatgccg cgattgctgg ccgcataaat ggcgcctctg gtatcaccgg tgatcgcata
     4201 gatcgtattg tcacgcaatc cgtttttggt atcaaaatta cgaataaccc cgtcgcgtat
     4261 caaagacaag ccgccaccat cggtaccaac gcacagaagg ccgtcgggcg cttcgtatat
     4321 cgaaacaaca aaatcgccgg ccagtccgtt tttctgagaa atatttttga attgcgatcc
     4381 gtcatatata aaaagcccgg cgccgtcgct gccaaaggcc atgcggccgt cacgcaacgt
     4441 acgtgcgcac catacgatct gcccggagag cggcgtcccg gtggcccatg gcacgatccg
     4501 acggtcttct attttagcaa gccctccgcg tgttgccaaa atcatgttcc cgtcggcatc
     4561 ttcggtcatt ccgtaagccc gattcatcgg tttatcggag tgatgataag tggcggtcac
     4621 gcgtccgtcg gttaccgtgt gaatgccgtt gtgtgtgccg atccacaacc gtccgcgacg
     4681 atcttcaaaa agactccaga ccaggttgtg cgccaatcct tttttttcgt cgaggatgtc
     4741 cggtttatcg ccacgcatgg tgatgaggcc ggcgtgtgaa ccaaacacca tactcccatc
     4801 gcgtctctga gtgaccgtgg tgaaaggcgc atcgggcaat tctttaccgc tgcgccacgt
     4861 cgtcaaacgc ccgggccgat acatgctgac gccgccgcca ttggtcccga aataaagcgt
     4921 acggtcatta tcttcataga tattccacac caaatcattg gccaatccgt tggccgtcgt
     4981 cagattctca aaaacgccgt ggtgccatag actgatgccg ttggctgtag cgatgcacaa
     5041 tgcgccgttt tgcaggagca gcatcgcatt gatgtcatta tcggcaagac cgtcgcgtgt
     5101 ggtgtagtta gtacaacgcc cgttgatata cacactcagg ccctcgcgtg tacccaaagc
     5161 ccatgcgcta tcattgagac ggcgaatgct gcgtacaatg gggtggatca gtccgtcgcg
     5221 tgccgtgatg cgctgttgcg tttgtgggga caggatcgct acaccgctgt cggtgccaaa
     5281 ccaccagaca tcgttaccgg cgtgaacgcc ggtgtacacg cgcagatcgg gtaacccttc
     5341 gtgaaggccg atggttttcc agcttcgccg ttgcggttga taaaaactga cgccgccgcc
     5401 ataggtacaa atccacaacg ccgaatcacc ggacggaatg atattccaca cgcgattatc
     5461 gcacaaaccg gaatctttat tgagtgtcgt aaaacgcccg tcgcgataca cagagaggcc
     5521 gccgccgtcg gtcccgatat acatagatcc gtcatggtac tcgtgcaacg ctgatatttg
     5581 tccgccggca agtccgtctt gggtttgaaa attttgaaaa cgaacaccgt cccaccggct
     5641 cagcccgccc aacgtgccga tccatacata ac
//
LOCUS       DAOQNQ010000212         5662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_5686_length_5662_cov_4.986802, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000212 DAOQNQ010000000
VERSION     DAOQNQ010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5686_length_5662_cov_4.986802"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2860)
                     /locus_tag="PK754_11745"
     CDS             complement(<1..2860)
                     /locus_tag="PK754_11745"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HNB10223.1"
                     /translation="MIRSIKKLLVANRGEIAIRIFRACSEIGIETIAVYTYEDRYSLH
                     RYKADEAYQIGADDDPLKPYLNIDEIIHLAKKVGADAIHPGYGFLSENDQFVKACEDH
                     GIIFVGPNSEIMYKLGNKIAAKVVARENNIPIIESNREALKDTSVAEREAQRIGYPVM
                     IKAAAGGGGRGMRVVRNADELRARFDEARGEARNAFGDDTVFIEKFIDDPKHLEVQIV
                     ADHFGNVVHLFERDCSVQRRFQKVVEIAPALTVSRDARERLYHYAVQIATAVGYQNIC
                     TVEFLLDKEENIYFIEVNPRIQVEHTVTEMITGVDLVKTQILIAQGYPLSDARLKLDD
                     QSRIYYNGYAIQCRITTEDPSNEFKPDYGTILAYRSAAGFGIRLDGGSIGPGIRISPF
                     FDSMLVKVSAWGLSFEETCDRMYRTLIEFRIRGVRTNIPFLENVVRHPIFREGRATIN
                     FIKDHPELLQFVKRRDRATRILRYIADVVVNGNPDVKIRDGKKVFIKPRIPAYDRYGD
                     FPDGAKQKLDTLGPEKFARWVMDQPKVMFTDCTMRDAHQSLLATRMRTYDIMRIAESW
                     AKHHSNMFSMEVWGGATFDVALRFLHEDPWDRLRQMRRAMPNTLLQMLIRGANAVGYT
                     AYPDNLIERFIIEAAREGIDVFRIFDSLNWTEGMKLSIETVRNKTGALAEACICYSGD
                     ILDPKRSKYSLTYYVELAKELERMGAHILAIKDMAGLLKPYAADVLIRALKDNIGIPI
                     HLHTHDTSSVQAATYVKAIEAGVDVVDVALSALSGTTSQPNFNSIVEMLRHTEHQVDV
                     DMTSLNAFSNYWEAVRDIYYPFETDLKAGTAEVYNHEIPGGQYSNMRPQAISLGLGEK
                     WEEIKQTYHDVNMLFGDIIKVTPSSKVVGDLAQYLVANNMTAADVLMKGETMSFPESV
                     EGFFMGHLGQPYGGFPEKLQKVIIKNKTPLTDRPGKHLP"
     gene            3167..3544
                     /locus_tag="PK754_11750"
     CDS             3167..3544
                     /locus_tag="PK754_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNB10224.1"
                     /translation="MRSKVFFAVCLLFGLMFINAGLNKFLNYIPVPPDLPEKMVKQSA
                     AFLEIGWLLPLVATVEIIGGILFIISKFRPLGAIIIFPVMIGIVMTHITVAPSGLPIA
                     LLLLAINLFIIYDNREKYIAMIK"
     gene            3579..4076
                     /locus_tag="PK754_11755"
     CDS             3579..4076
                     /locus_tag="PK754_11755"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNB10225.1"
                     /translation="MSNPVNVIKNLKEKFILVTKEFNAPLPNVWRTFSEKELLDQWWG
                     PSPWRAETKTMLFKPGGYWLYAMVGPDNQKHWGRMNYISIDLHHFIDMEDFFCDENGN
                     LNGELPISKGRIAFTKTESGTKVEFKTSYASETDIQKLVEMGFEQGITLCFEQLTQLL
                     NKKNL"
     gene            4073..4393
                     /locus_tag="PK754_11760"
     CDS             4073..4393
                     /locus_tag="PK754_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNB10226.1"
                     /translation="MRRDVFQAIADPTRRAIIGLIALQAMTPNAIAENFNSTRQAVSK
                     HLRILTECELVKQEYKGREIFYSLEIEKMKEIDDWLEQYRKIWESRFSQLDNLLSTMK
                     RKRK"
     gene            4390..4884
                     /locus_tag="PK754_11765"
     CDS             4390..4884
                     /locus_tag="PK754_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNB10227.1"
                     /translation="MNNLQFDFKVDKSTKTVFVEKEFDAELSLVWDAFTKKEILDQWW
                     APKPYASKTKYMNFKVGGRRFYAMVSPEGQENWHIQDYTSISPKTNFKFFSVFADKDE
                     NPFLPGSNWNLNFNENKETTKVSITIQNDSLERMEQMIEMGFKEGFTMTLNELSNLLS
                     NLKK"
     gene            4890..5504
                     /locus_tag="PK754_11770"
     CDS             4890..5504
                     /locus_tag="PK754_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB10228.1"
                     /translation="MKKHGLKIGIALLIVNTLGCGNSQPNSNPISKIDSADNKILTSE
                     HSMKTQKITPCLWVDKDAKAVVDYYLSIFKDGKMKDYRQYKNPPQENVPGGQDGFETA
                     IMEIGDIEFSILAAGPYFKFNESVSFVINCKEQAEVDYYWNALTTNGGEEGSCGWCKD
                     KYGLSWQVVPVEYFDLINSEDSKVREKAMRNTFKQKKLILSELK"
BASE COUNT         1683 a         1278 c         1202 g         1499 t
ORIGIN      
        1 gcggcaggtg tttgcccgga cgatcggtca gaggcgtttt atttttgata atgacttttt
       61 gcagtttttc cggaaaacca ccgtacggtt gtcccagatg ccccataaaa aatccttcca
      121 cagattcggg aaaggacatc gtttcccctt tcatcaaaac atcggcggcg gtcatattat
      181 tagccacgag gtattgcgcc agatcgccga ccactttgga tgaaggtgtt actttgatga
      241 tgtcgccgaa gagcatattt acatcatgat acgtttgttt gatttcttcc catttttcac
      301 cgagacccag tgaaatcgct tgcgggcgca tattgctgta ttgtccgccg gggatttcat
      361 gattatatac ttcggccgtt ccggctttaa gatccgtttc aaagggataa taaatatcgc
      421 gtacagcctc ccaataattg gaaaaggcgt taagcgaagt catatccaca tccacctgat
      481 gttccgtatg acgcaacatt tccacgatcg aattaaaatt gggttgggac gtcgtgccgg
      541 ataatgcgct gagcgcgaca tctactacgt ctacgccggc ttcgatagcc ttgacgtacg
      601 tagccgcttg cacggatgac gtatcgtgcg tatggaggtg aatgggaatg ccgatattgt
      661 ctttaagagc gcgtatcaaa acgtccgccg cgtacggctt tagcaatccc gccatgtctt
      721 tgatggccaa aatatgcgcg cccatacgtt cgagttcttt ggcaagctcg acataatatg
      781 tgagcgaata tttactgcgc ttcggatcga ggatatcgcc ggagtaacaa atgcatgctt
      841 cagcgagcgc accggtttta ttcctgacag tttcgatact cagcttcata ccttccgtcc
      901 agttgagcga atcaaaaatg cggaaaacat caatgccttc ccgcgccgcc tcaatgataa
      961 aacgttcgat cagattgtca ggataagccg tatagccgac ggcgttggca ccgcgaatca
     1021 acatctgcag cagcgtattg ggcatcgcgc gccgcatctg acgcaaacga tcccacggat
     1081 cttcatgcaa aaaacgcaac gccacatcaa aggtcgcacc gccccacact tccatgctaa
     1141 acatattcga atgatgttta gcccagcttt ccgcgatgcg cataatgtcg tacgtgcgca
     1201 tgcgtgtagc aagcaacgac tggtgcgcat cccgcatcgt acaatcggta aacatcacct
     1261 tcggttgatc catcacccat cgggcaaact tttccgggcc gagagtgtct aatttttgtt
     1321 tggccccgtc gggaaaatca ccgtaacggt cgtacgccgg aatacgcggt ttgatgaata
     1381 ctttttttcc atcgcgtatt ttgacatcag gattaccgtt gacaacgaca tcggcgatat
     1441 aacgcaaaat acgcgtcgca cgatcgcggc gtttgacaaa ctgcagcagt tcggggtgat
     1501 ctttgataaa attgatggtt gcacggcctt cacgaaaaat cggatgacgc acgacatttt
     1561 ccagaaaagg aatattagta cggacgccac ggatacgaaa ttcgatcaat gtacggtaca
     1621 tacgatcgca cgtttcctcg aaagacaaac cccacgcgga gactttgacc aacatcgaat
     1681 caaaaaaagg actgatacga atacccggcc cgatactgcc tccgtccaat cgaataccaa
     1741 aacctgccgc actgcggtag gccaagatcg taccgtaatc gggtttgaat tcgttactcg
     1801 gatcttccgt cgtaatgcgg cactggatcg catagccgtt gtaataaata cgcgattgat
     1861 cgtccagttt gagccgtgca tcgctcaatg gataaccctg agcgataaga atttgcgttt
     1921 tgactaagtc cacgcccgtg atcatttccg ttacggtatg ttccacctga atgcgcgggt
     1981 tgacttcgat gaaataaata ttctcttctt tgtcgaggag aaactccacg gtacagatat
     2041 tctggtaacc cacggcggtt gcgatttgta cggcatagtg ataaagacgt tcacgggcat
     2101 cgcggctgac ggtaagcgcc ggagctattt ctacgacttt ctggaaacgc cgctgcacac
     2161 tgcagtcacg ctcaaacaga tgaacgacat tgccgaaatg atccgcaacg atctgcactt
     2221 ccaagtgctt gggatcgtct ataaattttt cgataaacac cgtatcgtca ccaaacgcat
     2281 tgcgcgcttc gccccgtgct tcatcaaacc gcgcccgtag ctcgtctgca ttacgaacaa
     2341 cgcgcatacc gcgtccgccg ccgcccgccg ccgctttgat catcaccgga tatccgatgc
     2401 gctgcgcttc ccgttcggcc acggacgtat ccttcaacgc ctctcgatta ctttcgatga
     2461 tcggaatgtt attttcgcgc gcgactacct tggcagcaat tttattaccg agtttgtaca
     2521 tgatttccga attaggtccg acaaaaataa tcccgtgatc ttcgcacgct ttgacaaatt
     2581 gatcattttc ggaaagaaaa ccgtaaccgg gatgaatcgc atccgctccg acctttttcg
     2641 caaggtgaat gatctcatca atattcagat aaggcttcag tggatcgtcg tccgcaccga
     2701 tctgataggc ttcgtcagcc ttgtatcggt gcagggaata gcggtcttcg tatgtataaa
     2761 cagcaatggt ttcaattccg atttcgctgc atgcacgaaa aatgcgaatc gcaatttcac
     2821 cgcgattggc gacgaggagt tttttgatgg atcgtatcat aaacaggtag gtttgtgtaa
     2881 acgatgatcc cacaaagtta atgcattttg actgataaaa gcgaagaagt ttttttaaat
     2941 acaattcgtg cattatgacc gataccaaga cataccgtcc tataatcgaa acgacacaat
     3001 agaagcgtat aaactacatg cttttaaact atcacagacc caaccgataa aaatggatta
     3061 tccttttcaa aactttattt gaaaaaaaac ttgcgcaacc gaatagtttc atttatattg
     3121 caaccaataa gttgcgcatt ttttgtacac acgtgaaagt atgtatatga ggtcaaaagt
     3181 attttttgcg gtttgtctct tgttcgggtt gatgtttatc aacgccgggc tgaataaatt
     3241 tctcaattac atacccgttc cacccgattt acctgaaaag atggtaaaac agtctgccgc
     3301 gtttttggaa atcggatggc ttttacccct tgtggctacg gttgaaatca taggaggaat
     3361 tctttttatc atttcaaaat tcaggcccct cggcgctatc attattttcc ccgtgatgat
     3421 cggcattgta atgacacata ttaccgtggc gccttccgga ttaccgatcg cgcttctatt
     3481 gttagcgatc aacctcttta ttatttacga taaccgcgaa aaatacatag ccatgattaa
     3541 gtaactttta ttcacaacga agaaacccgg gagtaaatat gagtaatcct gtcaacgtaa
     3601 ttaaaaatct gaaagaaaaa ttcatcctcg taacaaaaga atttaatgcc ccattaccta
     3661 atgtctggcg caccttttca gagaaagaat tattggatca gtggtgggga ccttctccat
     3721 ggagagccga aaccaaaaca atgcttttta aacccggagg gtattggctg tatgccatgg
     3781 taggccccga caatcagaag cattgggggc gcatgaatta tatttcgatt gatcttcacc
     3841 acttcattga catggaggac tttttttgcg atgaaaatgg taatttgaac ggcgaacttc
     3901 ctatctcaaa aggaagaatc gctttcacta aaacggaaag cggaacaaaa gtcgagttca
     3961 aaacctccta tgcctccgaa acggatatcc aaaaactggt tgaaatgggc tttgaacaag
     4021 gtattactct gtgcttcgaa caattaactc aattactgaa taagaaaaac ctatgagacg
     4081 cgatgtattt caggctattg ccgacccgac ccgaagagcc atcatcggcc tcatcgcgct
     4141 gcaagcgatg acacccaatg ctattgcgga aaattttaat tcgacacgcc aggccgtttc
     4201 caaacacctg cggatattaa ccgaatgcga actggtaaaa caagaataca aaggaagaga
     4261 aattttctac tctttggaaa tcgaaaaaat gaaagaaatt gacgattggc tggaacaata
     4321 tcgaaaaata tgggaaagcc ggtttagtca acttgataat ctactctcaa ccatgaaaag
     4381 gaaaagaaaa tgaataattt gcaatttgac tttaaggttg acaaatcaac caaaactgta
     4441 ttcgtggaaa aagaatttga tgctgagctt tcacttgttt gggacgcctt tacaaagaaa
     4501 gaaattcttg accaatggtg ggcgccgaaa ccttacgctt caaaaacaaa gtatatgaac
     4561 ttcaaagtag gaggacgccg attttatgct atggtaagcc cggaaggaca agaaaactgg
     4621 catattcagg attatacttc cattagcccc aaaaccaatt tcaagttttt cagtgttttt
     4681 gcagataaag atgaaaaccc ttttttgccg ggctcaaatt ggaatttgaa tttcaacgaa
     4741 aataaagaaa caaccaaagt cagcattaca attcaaaacg attcgctcga acgcatggaa
     4801 caaatgatag agatgggctt caaagaaggt ttcactatga cattaaacga attatcaaac
     4861 ttattatcaa atttaaaaaa ataacgtata tgaaaaagca tggacttaaa attgggattg
     4921 ctttattaat tgttaatacc ctgggttgtg gaaattcgca accgaattca aaccctataa
     4981 gtaaaattga ttccgctgat aacaaaattt taacaagcga acactctatg aaaacacaaa
     5041 aaataacacc ttgcctttgg gttgataaag acgcaaaagc cgttgtcgat tattaccttt
     5101 ccattttcaa agacggcaaa atgaaagact atcggcaata taagaatccg ccgcaagaaa
     5161 atgtgccagg cgggcaagac ggtttcgaaa ctgcgattat ggaaattggt gatatagaat
     5221 ttagtatttt agccgcaggc ccatacttta agtttaacga atcggtttca ttcgttatca
     5281 attgcaaaga acaagccgaa gtagattatt attggaatgc gttgactacc aacggcggag
     5341 aagaaggatc ttgcggctgg tgcaaagaca aatacggttt atcgtggcag gtagtacccg
     5401 tggaatattt tgatcttatt aacagcgaag attctaaggt aagagaaaag gcaatgagaa
     5461 atacatttaa acagaaaaaa cttattttgt cagaacttaa ataataataa atcagatcat
     5521 gcacacgaaa aaactgaata ttttatcaat ttcgtttttg taagcaaatc cggcatctat
     5581 gcccacgcta aaggcttagc tgataagcct catttacaca tccacggcga tacacgttaa
     5641 cgtttgctcg acacccggta tc
//
LOCUS       DAOQNQ010000213         5570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_5842_length_5570_cov_6.178060, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000213 DAOQNQ010000000
VERSION     DAOQNQ010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5842_length_5570_cov_6.178060"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..743)
                     /locus_tag="PK754_11775"
     CDS             complement(126..743)
                     /locus_tag="PK754_11775"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNB10229.1"
                     /translation="MELSQFFEWIQSLDTGLIYLVIFLSGFLENCIPPIPGDMVTVLA
                     ASMVGVGRLSYFPTFLTATAGNLIGFMTMYQIGRFFGKDFFLSRNFKYFPATAFEKAE
                     AWFTRHGYRIILFNRFLSGLRSVISLFAGMTHLQKRKIIPLAFLSACMWDGVLIYAGY
                     VLGDNWKAFENLMAQYNRVVFSILGLIALILLIRWFIKRNSDHDR"
     gene            complement(749..1630)
                     /locus_tag="PK754_11780"
     CDS             complement(749..1630)
                     /locus_tag="PK754_11780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNB10230.1"
                     /translation="MIYSMTGFGKSERIFGSTSISVEMRSVNNRFLDLSWKSPRSFVL
                     KENEVREFLKRKLDRGKITVSLTIQSPDVNLASFKIDHSVFQQYASALREAAEKAGIA
                     QDVRLEHLLQYNDIFVSNNDAEAQEALWKNILTCIDEALDEMNRMRRSEGTELAKDFI
                     HRLETIETRLDKITELSKTAVTEELEKLRERVKLLLERTDFDPQRLDMELAILADKVD
                     ITEELVRFRSHNKMFRSLVEGKETQVGRKLNFIVQEMGRETNTIGSKSSQSEVIHTVV
                     SIKEELEKIREQIQNVE"
     gene            complement(1627..2616)
                     /gene="galE"
                     /locus_tag="PK754_11785"
     CDS             complement(1627..2616)
                     /gene="galE"
                     /locus_tag="PK754_11785"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012498604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNB10231.1"
                     /translation="MKILVTGGAGYIGSHAVRLLLRAGHDVVIFDNLIFGHADFAQGC
                     PLIQADLSDMAMLEKTFEQHKPQAVLHFAAYAYVGESVENPGKYYQNNVGSTLHLLEA
                     MVRHHVKKLVFSSTCATYGIPQKTPLTEDHPQAPINPYGQTKKMVEQILTDFDRAHGL
                     KSVCLRYFNASGADPDGGIGEDHNPETHLIPLVLDAAMGRRSQITVYGTDYETPDGTC
                     IRDYIHVTDLADAHIRALDFLTRENCSDFFNLGNGKGFSVKEIIAASEKVTGKNIPVH
                     YGERRPGDPAVLVGSAEKAKAILGWQPQYNDIHTIIQTAWNWHQSRFGSKRLV"
     gene            complement(2626..3108)
                     /locus_tag="PK754_11790"
     CDS             complement(2626..3108)
                     /locus_tag="PK754_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HNB10232.1"
                     /translation="MHSFDIVSKINMQELTNAVNQAVKEIQTRYDFKDSKSTIELGKD
                     EIVIHSDDDFKLKAVIDILQNKMIKRAVPIKALKYGKVEEASGSTVRQHIKLQNGIEQ
                     DQAKKIVAMIKDLKMKVQASIQGDQVRVQAKDIDDLQAIIKTLKEKNFDFAMQFENYR
                     "
     gene            complement(3183..3491)
                     /locus_tag="PK754_11795"
     CDS             complement(3183..3491)
                     /locus_tag="PK754_11795"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HNB10233.1"
                     /translation="MRKFKVCKESEIAEGQGRSIKVLAKEIMIYRIDGQLRAYDGLCK
                     HMRASLVSQSELKEYTLTCKWHGWQYDIRNGACLGKPDVYLTAHTIEITNGDIYVCVA
                     "
     gene            complement(3519..4688)
                     /locus_tag="PK754_11800"
     CDS             complement(3519..4688)
                     /locus_tag="PK754_11800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNB10234.1"
                     /translation="MKTIQLKPKHDHRIRNGHRWIFSNEIHDSLKSYEPGESVKITDA
                     NGYPVGVGYINPYSLIAVRILSFKPVEINRPFFLERIRNAYERRQVFYADQSCYRVVF
                     GESDDLPGLTIDRYGDYWVMQILTAGMDIHQDMIVDIIRSEFSPKAIILRNDSAVRKL
                     EHLPEEVRVAYGDCPSMVEVTIQNLLFRLDLLHGQKTGFFLDQKENHAYVRRFTSGKR
                     VLDCFTHLGGWAVHAALGGATEVIGLDISEAAIAQCRTHAEINQTTQCQFKCVDVFDE
                     LKAINDRRESFDVIILDPPAFAKSKSQVKEAIKGYREINRRAAKAIPSGGYLFTCSCS
                     QAIDPDTFRNTVFQGITAAGRQALLLEERTQPKDHPILMSMRETQYLKCLVIQII"
     gene            5067..5321
                     /gene="rpsT"
                     /locus_tag="PK754_11805"
     CDS             5067..5321
                     /gene="rpsT"
                     /locus_tag="PK754_11805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNB10235.1"
                     /translation="MPNHKSAVERVRRNKKQQDKNKNHKRQMKEAVKAVTTAKDKKTA
                     QDQLKQTVSVLDKLVVKGIIHKNKAANQKSKLAKKVQALA"
     gene            complement(5375..>5570)
                     /locus_tag="PK754_11810"
     CDS             complement(5375..>5570)
                     /locus_tag="PK754_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNB10236.1"
                     /translation="RHEIIMDRREAIRGGLQIAQDGDFVVIAGRGHERYQYVGDEKIP
                     FYDRQVFMEEAQKLELKVEE"
BASE COUNT         1500 a         1257 c         1264 g         1549 t
ORIGIN      
        1 attccggaga agcggcttgc gatacgaccg atagtttgat atcaacgatt tgttttttta
       61 tccacgcgga aggagcagaa gacgaacgaa ttgctgtgcg aagttcttgc agcgatgaat
      121 gaaaatcagc gatcatgatc ggaattacgt ttgatgaacc accggatcaa gagaataagt
      181 gcgatgagac ctaatatact aaaaaccacc cgattgtatt gtgccatcag attttcaaac
      241 gctttccagt tgtcaccgag tacatatcct gcgtaaatca aaacaccatc ccacatgcac
      301 gcgctcagga atgcgagcgg aatgatctta cgtttctgaa gatgtgtcat acccgcaaaa
      361 agtgaaatga cagagcgtaa accgctcaaa aaacgattaa aaagaattat acggtatccg
      421 tggcgggtaa accatgcctc ggctttttca aacgccgtcg caggaaaata cttaaaatta
      481 cgagacagga aaaaatcctt accgaaaaag cgaccgatct gatacatcgt cataaaaccg
      541 attaaattac cggctgttgc cgtcagaaaa gtaggaaagt aagacaggcg tcctacgccg
      601 accatgctgg ccgctaacac cgtcaccatg tcgcccggaa tcggaggaat acagttttcc
      661 aaaaagccgc tcagaaaaat aaccaagtaa atcaaaccgg tatcgagcga ctgtatccat
      721 tcaaaaaact gactgagttc catatacgtt attcgacgtt ctgtatttgt tcgcggattt
      781 tttcaagctc ttctttgatg gatacgacgg tgtggatcac ctcggactgg gatgatttgg
      841 aaccgatggt gttggtttcg cgtcccatct cttgtacgat aaaattgagt ttgcggccga
      901 cctgtgtttc tttgccctcg acaagagaac gaaacatctt attatggctg cgaaatcgaa
      961 ccaattcttc cgtgatatcc actttatcgg cgagtatggc cagttccatg tcaagtcgct
     1021 gaggatcaaa atcggtgcgt tctaatagaa gttttacgcg ctcacgtagc ttttcaagtt
     1081 cttcagtgac ggctgttttg gataattcgg tgattttgtc caaacgggtt tcgatcgttt
     1141 cgagccgatg gatgaaatcc ttcgccagct ctgttccttc actgcgacgc atgcggttca
     1201 tttcatccag cgcttcgtca atacaagtta aaatattttt ccaaagcgct tcttgggcct
     1261 cggcatcgtt attggaaaca aaaatatcat tgtactgcag aagatgctct aaacgtacat
     1321 cctgtgctat accggccttt tccgccgctt cgcgtaaggc ggaagcgtat tgctgaaaca
     1381 cgctgtgatc aattttaaac gaagccagat tgacatccgg agactgaatg gtcaatgata
     1441 ccgtgatttt accgcgatca agtttgcgtt tcagaaattc acgcacctcg ttttctttaa
     1501 gaacaaaact gcgcggtgat ttccacgaaa gatctaaaaa acgattgttg acggaacgca
     1561 tttcgacgga aatgctggtt gaaccgaaga tgcgctccga tttgccgaag ccggtcatgc
     1621 tgtaaatcat acgaggcgtt tgctcccaaa gcgggactga tgccagttcc acgctgtttg
     1681 tattatagta tgtatatcgt tgtattgcgg ttgccatccg agtatggctt tagccttttc
     1741 agcacttccg acaagcacgg ccggatctcc tggcctgcgt tcgccgtagt gaacgggtat
     1801 atttttgcct gttacttttt cacttgcggc aataatctct ttgactgaaa aacctttacc
     1861 gtttccgagg ttaaaaaaat cactacagtt ctcacgcgtc aaaaagtcta acgctctaat
     1921 atgggcgtcg gcaagatcgg taacgtgaat ataatcgcga atgcatgtgc cgtcgggtgt
     1981 ttcataatcg gtaccgtaaa cagtgatctg cgatcggcga cccatcgcag catccaatac
     2041 gagcggaatg aggtgcgttt cgggattgtg atcctcaccg ataccgccat ccggatcggc
     2101 gccggaggca ttaaaatacc gaaggcacac cgacttcaaa ccatgcgccc gatcaaagtc
     2161 cgtcaaaatt tgctctacca tttttttggt ttgcccgtag ggattgatcg gcgcctgagg
     2221 gtgatcttcc gtcaatggcg ttttttgcgg tataccgtac gtcgcacagg tggatgaaaa
     2281 aactaatttt ttaacatgat gccgcaccat cgcttctaaa agatggagcg ttgacccgac
     2341 attgttttga taatatttcc cgggattttc gaccgattcg ccgacgtaag catacgctgc
     2401 aaaatgaagc acggcctgcg gtttgtgttg ttcaaaagtc ttttcgagca tcgccatatc
     2461 tgatagatcg gcctgaatca acggacagcc ctgtgcaaaa tccgcatgcc cgaaaatcag
     2521 gttatcaaaa attacgacat catgtccggc gcggagtaac aggcgaacgg catgcgaacc
     2581 gatgtatccg gcgccgccgg tgacgaggat tttcatgatg tacgcttatc tgtagttttc
     2641 aaattgcatg gcaaaatcaa aattcttttc tttgagcgtt ttgatgatag cctgcagatc
     2701 atcaatgtct ttggcctgta cgcgcacctg atcaccctga attgaggctt gcactttcat
     2761 cttgagatct ttgatcattg cgacgatttt tttggcctga tcctgttcga tcccgttttg
     2821 caatttgata tgctgacgta ccgtgctgcc ggaagcttct tcgactttcc catatttcaa
     2881 ggctttgatc ggcacggcgc gtttgatcat tttgttttgc aaaatatcaa tgaccgcttt
     2941 gagtttaaaa tcatcatcgg aatggataac gatttcgtct tttccgagtt cgatcgtact
     3001 tttgctgtct ttaaaatcat agcgcgtctg aatttcttta acggcctgat tgacagcatt
     3061 agtaagctct tgcatattga ttttggaaac gatatcaaac gaatgcatgt ccgccataaa
     3121 atctctcctt ttattcgcgg ggcaatttaa cataatacgg cataaaaaca atatccaagc
     3181 ggttatgcca cgcacacata gatgtcgccg tttgttattt cgatggtgtg cgcagtgagg
     3241 tacacatccg gtttaccgag gcacgcaccg ttgcggatgt cgtattgcca gccgtgccac
     3301 ttgcaggtca gcgtgtactc ttttagctcg ctctgcgaaa ccaacgatgc gcgcatgtgt
     3361 ttgcataaac catcgtaggc gcgcagttga ccgtcaatac gatagatcat gatttctttg
     3421 gctaatacct tgatggaacg gccctgacct tcagcgattt cggattcttt gcagacttta
     3481 aattttcgca taaatgtatg ataaggcgag ggtatcggtt aaataatttg aatgactaaa
     3541 catttgagat attgcgtttc gcgcatactc ataagtatcg gatggtcttt gggctgtgtg
     3601 cgttcttcca agagcaacgc ctgtcgaccg gctgcagtga ttccttgaaa taccgtattc
     3661 cgaaacgtat cgggatcaat cgcttgcgaa caactgcaag taaataaata gccgccggag
     3721 ggaatcgcct tggccgcacg acgattgatt tcacggtatc ctttgatggc ttctttgact
     3781 tgcgatttag atttggcgaa tgcgggcggg tcaaggatga tgacatcaaa cgattccctg
     3841 cgatcgttga ttgctttgag ttcatcaaaa acgtcaacgc atttaaattg gcattgtgta
     3901 gtttgattga tttccgcatg ggtacggcat tgggcgatgg ccgcttcgga aatatccagt
     3961 ccgataactt ccgtcgcacc gccgagcgcg gcatgcacgg cccatccgcc aagatgcgta
     4021 aagcagtcga gtactctttt tccggatgta aaacgcctta cataagcatg attttctttt
     4081 tgatccaaaa aaaatccggt cttttggcca tgcagcagat cgaggcgaaa cagcaggttt
     4141 tgaatcgtca cttcgaccat cgaaggacaa tcaccatacg ctacacgcac ttcttcaggc
     4201 aagtgttcaa gttttcgcac agcggagtca ttgcgcagaa taatcgcttt gggcgaaaat
     4261 tcactgcgaa tgatatctac gatcatgtct tgatggatat ccatgcctgc ggtgagaatt
     4321 tgcataaccc aataatcgcc gtaccggtct atagttaggc ccggcagatc atcggattcg
     4381 ccgaaaacga cccggtaaca ggattggtcc gcgtaaaaaa cctgtcgccg ctcgtaagcg
     4441 ttccggatgc gttcgagaaa aaatggacga ttgatttcaa ccggtttaaa gctcaggatg
     4501 cgcacggcaa taagcgaata gggattaata tatcccacac cgacaggata accgttggca
     4561 tccgttattt tgacggactc gccgggctca tacgatttga gcgagtcatg gatttcgttg
     4621 ctgaatatcc accgatgacc gttgcgaatg cggtgatcgt gtttgggctt gagttgtata
     4681 gttttcatgg cggacgaaaa tatatgattt gcgtgtgata agcaaacgta agaaggtttg
     4741 ccgtgattta agatcgtatg ggtaaatgag atacacccgt tcattcatat gctaagagcg
     4801 gtttttacgg cgtacttgat tacatactat aaaacacatg agaattattt tcatgctcag
     4861 acactgtatt ttgtgatccg acgaaagatt taacgctacg attgtggaag tgttgcttac
     4921 aataaaattt acgttgaccg taacaagttt ttcaaagata aaccgaggta atttttttga
     4981 attttctgat tgacaatcct tattgagttg tatatcttcg cccagtttta gaataatatt
     5041 aaacctaacg ttataggagt tttgcaatgc ccaatcataa gtccgcggtc gagcgtgttc
     5101 gccgaaacaa aaagcagcag gacaaaaaca aaaatcacaa acgccagatg aaagaggcgg
     5161 tcaaagcggt caccacggct aaagacaaaa agacggccca ggatcaattg aaacaaaccg
     5221 tcagcgtact ggacaaactt gtggtcaaag gtatcatcca caagaacaaa gcggccaatc
     5281 aaaaatcgaa attagctaaa aaagtgcaag ctttggctta agtttttatt ttgtagtaat
     5341 aaaaaagccc ggtgcattcg catcgggctt ttttttattc ttctactttt aattcaagtt
     5401 tttgtgcttc ctccataaag acctgccggt cataaaacgg gattttttca tcccccacat
     5461 actggtagcg ctcatggcca cgtcctgcga tgactacaaa atcaccatct tgcgctattt
     5521 gaagtccacc acgaatagct tcacgtcgat ccatgatgat ttcatgccgg
//
LOCUS       DAOQNQ010000214         5511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_5926_length_5511_cov_4.845858, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000214 DAOQNQ010000000
VERSION     DAOQNQ010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5926_length_5511_cov_4.845858"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /gene="ffh"
                     /locus_tag="PK754_11815"
     CDS             <1..1048
                     /gene="ffh"
                     /locus_tag="PK754_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNB10237.1"
                     /translation="IPPTVVMVVGLQGSGKTTFSGKLANYLRKKGRKPMLVAADVYRP
                     AAADQLRTLGKTLDIPTFSPDEAGSHDPVKICFDAVTYARQHGRDLIVLDTAGRLHID
                     DALMTELEQIKIKTQPNEILFVADGMTGQDAVNTAAEFVKRLDFDGVVLTKLDGDARG
                     GAALSIRSITGKPIKFIGTGEKMDALEEFYPDRMASRILGMGDIVSLVEKAQETIDQE
                     QAQKMHEKMMKNEFTLADFSTQLGQIKKLGSLESILSMIPGVGSKIKGMQMDDKVLVR
                     MEAIISSMTPKERERPQMLDGRRRLRIARGSGTSVQEVNRLLKQFDDMKRMMKKIGKM
                     NPAEVMNMMSQMGR"
     gene            1100..2785
                     /locus_tag="PK754_11820"
     CDS             1100..2785
                     /locus_tag="PK754_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10238.1"
                     /translation="MKTWIILLSFTAIANAQNSELYFQSDSERALFKDSIFFKTNNST
                     EVFENLLITEGVSDSSSIRKYRGYLEKIAARLSKEINLKSKASKKAEVIFTYLHKNIF
                     KKYNIDTRFQDLFSRGEYNCVTATYLYYDMCKRFDLNVTFYASAVHIYPVVKGEQRPI
                     NVELTDPVNGFDFREDRKAIINYLLEYKLITQDELKSKGSDAVYREYVDKTKEVGIPA
                     LYSISYSNQGISYWQAGKLKETLWAMEKALSIIWDDSKSRAIYQSVFDENLNKILYDV
                     PSAYPVVIRGLQFMRSDTSFVRDHFNFIMHAIEYYSVTKLEYDKALEIVNEGKAIFSF
                     DTSLVKRLTEFSRDVRWNQFAYVYNASNYEKAAAILEIGLSEFPNDVRFLDFQKKTDL
                     LLAERLSDKGDYQGAINRLSGLRKKYSSDFRIEDTYVGTVTYFASKCAQNGESKKAID
                     LMDSLASSLPENFKVKESYSAIVGLIISTEELDKKNIYEARRLIIKAIQVDTKNITMT
                     KMLAAIYHELAMAKVRKKEYLAAKNLILEGLGYIPQSYELNDDLKLIKQQLGK"
     gene            2923..3324
                     /gene="rpsP"
                     /locus_tag="PK754_11825"
     CDS             2923..3324
                     /gene="rpsP"
                     /locus_tag="PK754_11825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNB10239.1"
                     /translation="MVKIRMQRGGKAHQPFYRIVAAHSTRKRDGDYIERIGSYNPLTT
                     PPQITIDEKKALKWLTDGAQPSDTVKTLFRSAGILHRWRLTAKGLSVEEVNAEMEKWK
                     ANQAKKAAEKRAKRTKAVKKKTEKASEGSAA"
     gene            3370..3600
                     /locus_tag="PK754_11830"
     CDS             3370..3600
                     /locus_tag="PK754_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNB10240.1"
                     /translation="MQSFIEFVVKHLVDKPDEVEVKEVDGERVVVYELKVGQGDLGKV
                     IGKRGQNAKALRTLLAAASARAGKRAVLEILE"
     gene            3607..4134
                     /gene="rimM"
                     /locus_tag="PK754_11835"
     CDS             3607..4134
                     /gene="rimM"
                     /locus_tag="PK754_11835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNB10241.1"
                     /translation="MQSEALIQVGRILRSHGLRGELKIEVFNDGLEELESGTPCVLKS
                     RFGKQAEMHIERIRFGNDTILKLREMQDKSSADVWSQGEIWLLEKLLPALPEDAFYVK
                     DITGLEVRDESGKKIGILADVETSPAHDLYVIEVQGKRYRIPAVAEFIKAINKETGVI
                     TIHLIPGLLEYQTAS"
     gene            4156..4899
                     /gene="trmD"
                     /locus_tag="PK754_11840"
     CDS             4156..4899
                     /gene="trmD"
                     /locus_tag="PK754_11840"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009594990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNB10242.1"
                     /translation="MSTLRIDIITAFPKMIDDALGHSIVGRAAKKGLVQIRAIDLRTY
                     TQDRHRTIDDTPYGGGGGMLIKPEPMFACIEDVLQIPPITSANHVRDVISPDTDVIMT
                     SPMGQTFDQKKAVEFSLKKHLVFICGHYKGIDERVHEKLVTQSVSIGDYVLTGGEIPV
                     LAMVDATVRLLPGAIGDAQSALTDSFQEGVLDCPYYTRPENFRGMKVPEVLLQGHHEA
                     IQTWREEQRLAMTRKLRPDLIRKEEVLKK"
     gene            4928..5302
                     /gene="rplS"
                     /locus_tag="PK754_11845"
     CDS             4928..5302
                     /gene="rplS"
                     /locus_tag="PK754_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020615313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNB10243.1"
                     /translation="MNKVDAFTAFQLRNDIPEFRAGDTVAVDVKVVEGDKERIQTFEG
                     VVIQRRGSGIHESFTVRKISNGVGVERIFLANSPRLGTVEVLKRGRVRRAKLYYIRGR
                     RGKSARIQDIVDTTETAQSAAK"
BASE COUNT         1625 a         1063 c         1348 g         1475 t
ORIGIN      
        1 cattcctcct acggtagtga tggtcgttgg tttgcaaggt tccggtaaga cgacgttttc
       61 cggcaaatta gcaaactatc tgagaaaaaa aggccgtaag cccatgctgg tggcggcgga
      121 cgtatatcgt ccggccgcgg ctgatcagtt gcgaaccctc ggcaaaacgc tcgatattcc
      181 gacattttct cccgacgaag cgggttccca tgatccggtc aaaatctgtt ttgatgcggt
      241 gacctatgca cggcaacacg ggcgcgatct gatcgtgctc gatacggccg gtcgtttgca
      301 tattgatgat gcattgatga cggagctcga acagatcaaa attaaaacgc agccgaacga
      361 aattttgttt gtcgccgacg gtatgaccgg acaggacgcc gttaataccg cggccgagtt
      421 tgtcaaacga ttggattttg acggtgtagt actgaccaag ttggacggtg atgcacgcgg
      481 cggtgcggca ttatctatac gcagtattac gggaaaaccg atcaaattta tcggtaccgg
      541 cgaaaaaatg gatgcgctgg aagaatttta tccggatcgc atggcgtcgc gtattttggg
      601 tatgggcgac atcgtcagtc tcgtggaaaa agcgcaagag acgattgatc aagagcaggc
      661 ccagaagatg cacgagaaga tgatgaaaaa cgagtttacg ctcgccgatt tttcgacgca
      721 actcgggcag atcaaaaaac tcggttcgct ggaatctatt ctttcgatga taccgggtgt
      781 cggcagcaaa atcaaaggca tgcaaatgga cgataaagtt ttggttcgca tggaggcgat
      841 tatttcttcc atgaccccca aagaacgcga acgtccgcag atgctggacg gccgccgccg
      901 tttgcgtatt gcccgcggca gcggcacttc ggtacaggaa gtgaaccgtc tgcttaaaca
      961 atttgatgat atgaaacgca tgatgaaaaa gatcggaaaa atgaatccgg ctgaagtgat
     1021 gaatatgatg tcgcaaatgg ggcgataggg ttggttttat gatcggattc tcttgtataa
     1081 catgggtgag gggaaatcaa tgaaaacgtg gattatttta ttgagcttta cggctattgc
     1141 caatgctcaa aattcggaat tgtattttca aagcgactcg gaacgggcct tgttcaagga
     1201 tagtattttt ttcaaaacta acaattcgac ggaagttttt gaaaatttac tgataactga
     1261 aggcgtatca gacagttctt cgattcggaa atatcgaggg tacttggaga agatagccgc
     1321 ccgtctttcc aaagaaatta atctgaaatc taaggcctca aaaaaggcag aagttatttt
     1381 tacatacctc cataagaata tttttaaaaa atacaacatt gatacccgat ttcaggatct
     1441 cttttcgcgc ggtgagtaca attgtgttac ggcgacttat ttgtattatg atatgtgtaa
     1501 gcgtttcgat ctaaatgtta ctttttacgc atcggcagtt cacatttatc cggtagtaaa
     1561 gggagaacag cgtccgataa atgtagaatt gacagatcct gtaaatggtt ttgattttcg
     1621 tgaagatcgc aaagctatta tcaattatct tcttgagtat aaattgatta ctcaagatga
     1681 actaaaatct aaaggctcag acgctgtata tagagaatac gtagataaaa caaaagaagt
     1741 tgggattcct gcgttgtatt ccatttcgta ctccaatcaa ggtatatctt actggcaagc
     1801 tggcaaatta aaagagacat tatgggcgat ggaaaaggcc ctttcaataa tctgggatga
     1861 ctccaaaagc cgtgcaattt atcaatctgt attcgatgaa aatctaaata agattctgta
     1921 tgatgtacca tcggcgtatc cggttgtaat tagaggtctt caattcatgc gctcggatac
     1981 ctcttttgtg cgtgatcatt tcaattttat tatgcatgct atcgaatatt attcagttac
     2041 aaaattggaa tatgataaag cattagaaat agtaaatgaa gggaaggcga ttttttcttt
     2101 tgacacttct ttggttaagc gtctgaccga gttttctcgc gatgttcgct ggaatcaatt
     2161 tgcttacgtt tacaatgcta gcaattatga aaaagcggcc gcaattcttg aaatagggtt
     2221 aagcgaattt cctaacgatg tgcgtttttt agatttccag aaaaaaacag accttttgct
     2281 ggcagagcgt ttgtccgata agggagatta tcagggagct ataaatcgtt tatcgggctt
     2341 aaggaagaaa tattcatccg attttagaat cgaagataca tacgtgggta cagttacata
     2401 ttttgcttcc aaatgtgcgc aaaacggaga aagtaaaaag gccattgatt tgatggattc
     2461 tcttgcgtct tctttgccag agaatttcaa ggtaaaggaa tcctattccg ctatagttgg
     2521 attaatcata tcaacagaag aacttgacaa aaaaaatatt tatgaagcac gacggttaat
     2581 aattaaagcg atacaagtcg acacaaaaaa tataacaatg acaaaaatgc tcgctgcgat
     2641 ataccatgaa ttagccatgg caaaggttcg taagaaagag tatttggcgg ccaaaaattt
     2701 aatacttgaa gggctaggat atataccgca aagttatgaa ttgaatgacg atttaaagct
     2761 cattaaacag caacttggca agtaaagtat agattaaaaa aaatcttgcg ttaatccttt
     2821 gtttttttta tgatgtgcgc ctaaagtgaa aatgtgttaa gtataaaaag tttagtagtt
     2881 tatatataaa attaacgtac atcgaattgg aggtaaaata ccttggttaa gatcagaatg
     2941 cagcgcggag gcaaagccca tcaaccgttt tatcgtatag tagcggcgca cagcacgcgt
     3001 aaacgcgacg gtgattatat cgaacgtatt ggcagttata atccgctgac gactccgccg
     3061 caaatcacga ttgacgagaa gaaagcgctg aaatggctta cggatggtgc acaaccttcg
     3121 gatactgtaa agacattatt ccgcagtgcg gggattctgc accgctggcg cctgacggcg
     3181 aaaggtcttt ccgtcgaaga agtcaacgcc gaaatggaaa agtggaaagc caatcaagct
     3241 aaaaaagccg cggaaaaacg tgccaaacgt acaaaagcgg tgaagaaaaa aactgaaaaa
     3301 gcttctgaag gatctgcggc ttaattcata caggactggt ctgcaactaa cttctaagga
     3361 ggaatgacga tgcagagctt cattgaattt gtggtcaaac acctggttga taaacccgat
     3421 gaagtcgaag tcaaagaggt ggacggtgaa cgtgtagtcg tttacgaact caaggtaggc
     3481 caaggcgacc tgggcaaagt gatcggcaaa cgcggacaaa acgccaaagc cttacgaact
     3541 ctgctcgcgg ccgcttcggc acgcgccggt aaacgcgctg tgctggagat cctggagtaa
     3601 ctgtacgtgc aatccgaggc attaattcag gtcggtcgta ttttgcgcag ccacggttta
     3661 cgtggcgaac ttaagatcga agttttcaac gacggtctgg aagaattgga atccggtacg
     3721 ccctgtgttt tgaagtcgcg gttcggtaaa caagcggaaa tgcatattga gcgcatacgt
     3781 tttggtaatg atacgatcct gaaattgcgt gagatgcagg ataagtcttc cgccgatgtt
     3841 tggtcgcaag gcgaaatatg gttactcgaa aagttgttgc cggctttacc cgaagatgct
     3901 ttttatgtga aggatattac gggtttggaa gtgcgggacg aatccggaaa aaaaatagga
     3961 attttagccg atgtcgaaac ctcacctgca catgatctgt atgtcatcga ggtgcaaggc
     4021 aaaagataca gaattccggc cgttgctgaa tttattaagg ctatcaataa agaaaccggc
     4081 gttattacaa tacatctgat tccgggtttg ctggaatatc agacggcctc ctaaacggag
     4141 cgatgttctt ttacaatgag tactttacgt atagacatca tcacggcatt tccgaaaatg
     4201 atcgacgatg cactgggtca cagtatcgtg gggcgtgcag ccaaaaaagg gctggtacag
     4261 attcgtgcca ttgatctgcg tacatacacg caagatcggc atcgcaccat agacgataca
     4321 ccttacggcg gcggcggcgg tatgcttatc aaaccggagc cgatgtttgc ttgtattgag
     4381 gatgtgcttc agattcctcc gatcaccagt gcaaatcatg tacgcgatgt gattagtccg
     4441 gatacggatg taattatgac ctcaccgatg gggcaaacat ttgaccagaa aaaagccgtg
     4501 gaattttcat tgaaaaaaca tctggttttt atttgcggcc attacaaagg tatagacgaa
     4561 agggtccatg aaaaactagt tacccaatcg gtttccatcg gagattatgt tctgaccggc
     4621 ggcgagatac cggttttggc aatggtggat gcgacggttc gacttttacc gggtgccatc
     4681 ggtgatgcgc agtcggcgtt gacggattcg tttcaggaag gtgttttaga ttgcccgtat
     4741 tatacgcggc cggaaaactt tcgtggaatg aaagtacctg aggtattact gcaagggcat
     4801 cacgaagcga tacaaacatg gcgggaagag caaaggttgg caatgacgcg taagcttcgt
     4861 cccgatttga tacgtaaaga agaagtatta aaaaaataat ttaacaatac atattttgga
     4921 ggaacccatg aacaaagtag atgcctttac ggcattccag ttgcgtaacg atataccgga
     4981 atttcgcgca ggcgatacgg ttgctgtaga tgtaaaagta gttgaaggcg ataaagaacg
     5041 tattcagacg tttgaaggcg tcgtaattca acgccgtgga agtggtatcc atgaatcatt
     5101 caccgttcgc aaaatttcaa acggtgtcgg cgtggaacgt atctttttgg ctaattcgcc
     5161 ccgccttggt accgtcgaag ttttgaagcg tggtcgtgtt cgtcgggcca aactttatta
     5221 tatccgcggt cgccgtggta agagtgcacg tattcaggat attgtggata caacggaaac
     5281 ggcacaatcg gccgcaaaat aagtttcgac ttttattgaa taagggtatg cgatcggcat
     5341 accctttttt tttccttatt cggttccatt gccattgtta cgacatttaa catgatgcat
     5401 cgtatcactc cttatttgac tattatacga ccgctcaatg cgttgatttc atttctctcg
     5461 ccgatgttga tcctcgtgat cagcgatgca tcagaagatg aagtcgcatg g
//
LOCUS       DAOQNQ010000215         5460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_6021_length_5460_cov_5.560037, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000215 DAOQNQ010000000
VERSION     DAOQNQ010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6021_length_5460_cov_5.560037"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PK754_11850"
     CDS             <1..419
                     /locus_tag="PK754_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNB10244.1"
                     /translation="AGIDRIKTNVPYREVADAITQTLEDGLLEIGLIQDRKEAKKYTV
                     HGYMHSIGLDVHDPQSYDKPFVPGFFTTVEPGIYIPENAPCDKKWWNIGIRIEDDILV
                     TESGNINLSERVPRKIEDIEKLMKKKGVGNVPLDNN"
     gene            429..2702
                     /locus_tag="PK754_11855"
     CDS             429..2702
                     /locus_tag="PK754_11855"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNB10245.1"
                     /translation="MYRVKLEQFEGPLDLLLFFVRKDELNIYDIPIAYITKQYLEYLH
                     LMRTLNLDIAGEFILMAATLMRIKAKMMLPPDPTTEEEEELIDPRQELSRRLVEYRQF
                     KEASKSLAELDEYWRSVYRRSYFNFDLIPQQTEEAVGLKDISFFDLLTAYKNAMAKKP
                     AVIYHNVERLNVTVEEQQEYIMDFFRDRNMYTFLELVEDMSKIEVVVTFLALLDLVKK
                     GEIAARQTTLFDDIWIYKASAYTDEIPDELRAEESETEKTIPSNDEPVLTPSEAPVLN
                     EVSSNDTTFAINESGDTALDTSLIEVAASPDSELANDHIDMAQTDGVVQEVEQAAVEM
                     SIESVIEAAKTDAETVELNEEILNNEAFVSAPQTEEQPQTEYTDTSEAFADSENDEII
                     GATVEENLNSLSDENIYSVEEIRSNSSSDETISVSVNESTEVDFYTQVPSEVTSAEQN
                     QSDISDVKTENMGSHGEDFSHEETELLDRTIENVSDDGIATDLTASISDATVDGNVTD
                     DAVLLISTTEDQTIASPVPVELIRTVIAESQVNVNNSDLESADEITGNLALSDTEDKV
                     IVPTNEPIVGEAETNTTDIDQTPPQVSSNEIGDNINADQSASDTIISVEENEPALIRS
                     NDVSTPDIQAIHENELTSEDVVESITSEPPQNTDPVHIIEAIHNQNYETIQPDLVKPL
                     VSQDAPRTDGESVVSAKMEDDVSKPLVEQHETADAFLSRTTTNDEKPSLDETEKPSII
                     TVFVRKIVSFVKRFFGK"
     gene            2721..3524
                     /gene="scpB"
                     /locus_tag="PK754_11860"
     CDS             2721..3524
                     /gene="scpB"
                     /locus_tag="PK754_11860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNB10246.1"
                     /translation="MEEQNQNESNLEPTEVQIDTETSEVPADPRIVDGLPLQNVVESI
                     IFASTEPLPMDRLLECIQKDYVQASLVRQVIDEINKHYQANNLPLRVIKIAGGYQYAT
                     HPQYDKWVSRLFKSKAEKKLSQSSLEVLAIVAYRQPISRAEIERIRGVNADWTLRSLM
                     EKNLITVVGREDAPGKPLLFGTTKPFLEHFGLDAINELPKLKEIEDIIKEDKEFAETL
                     EFDFADKKAAEAAAKPADETAKNEGLSQEFAEIDTILKENNFNPEDHLK"
     gene            3531..4364
                     /locus_tag="PK754_11865"
     CDS             3531..4364
                     /locus_tag="PK754_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10247.1"
                     /translation="MNFDYLNDQTWIVNQDIPWKHLSKDLSEVRIAIITTAGLYMWKT
                     QKPFSFEDEIPDVSFREIPNSVTQETVRVSHRYMDLFGSAGVDINSVFPIHRLQEMVS
                     EKRIGGLAETHYSVMGEVRDPRLFIEDCMPKILKLLQKQLVDVVLVTPGGPLGHQTAG
                     MIARFLEENDITTITIGAVKAIAQNVKSPRTLLVRYPFGMYFGAPFDTVNQKEIVNEC
                     LVHLKAIREPGEVSELSVRWQDTFKTALQIKPELPTLMDTGAIIAEPVIEKKKIAADE
                     F"
     gene            4371..5081
                     /locus_tag="PK754_11870"
     CDS             4371..5081
                     /locus_tag="PK754_11870"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005386768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNB10248.1"
                     /translation="MRLNKFMATAGVASRRSCDEIIGEGRVTVNGQTVTDLSYQVDEA
                     LDRVEVDGQLLSIKEKDVYIILHKPLRYVTTVKDERGRDSVVDLVKITERVYPVGRLD
                     FDTTGLLLLTNDGELAFRLAHPKFGIEKTYIALLNKKIGESELQQLRRGVVLIDGKTA
                     PCKAEKLGKELVKITIHEGKNKQVKRMFRKLGYKVRELHRSQYGPIQLGKLKYGQWRN
                     LKPAEVLALKKATGLIKK"
     gene            5092..>5460
                     /locus_tag="PK754_11875"
     CDS             5092..>5460
                     /locus_tag="PK754_11875"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNB10249.1"
                     /translation="MKKLLITLDGPAGSGKSTTAKLLAKRLGYCYLDTGAMYRAITLQ
                     AIHHGIFPTDPEKIIAMLPDITLHIDYKDGRQQTFLNGWDVSEGIRSPEVTRHVSAVS
                     AIAEVRTFLRQQQQHIGNQGG"
BASE COUNT         1661 a         1115 c         1306 g         1378 t
ORIGIN      
        1 aggcgggtat tgaccgcatt aaaacgaatg taccttaccg tgaagtagcg gatgccatta
       61 cccaaacatt ggaagacggt cttttggaaa tcggattgat tcaggatcgc aaagaagcaa
      121 aaaaatatac cgttcacggc tatatgcatt cgatcggact ggacgtgcat gatccgcagt
      181 cgtatgacaa gccgttcgtt ccgggttttt ttacgacggt agaaccgggt atttatattc
      241 ctgaaaatgc accgtgcgat aaaaaatggt ggaatatcgg tatccgcatt gaagatgata
      301 ttttggtcac ggaaagcggc aatatcaatc tgtctgagcg tgtacctcgc aaaatagaag
      361 acatcgaaaa acttatgaag aaaaagggtg ttggtaatgt accgttggat aataattgag
      421 ggcgcatcgt gtatcgtgta aagttagaac aatttgaagg ccctttggat ttgttgttat
      481 tcttcgttcg taaggacgaa ctgaatattt acgatattcc tatcgcatac atcaccaagc
      541 agtatctcga atacctgcat ttgatgcgta cgctcaattt ggatattgcg ggtgagttta
      601 ttctgatggc cgccacgttg atgcgtatca aggccaaaat gatgttaccg ccggatccga
      661 cgaccgaaga agaagaggaa cttatcgatc cgcgtcagga attgtcacgt cggcttgtcg
      721 aataccgtca gtttaaagaa gcttccaaat cattggccga gctggatgag tattggcgct
      781 ccgtatatcg ccggagctat ttcaattttg atctgatccc tcagcaaacc gaagaagcgg
      841 ttggtttaaa agatatctcc ttttttgatc ttttgacggc ttataaaaat gcaatggcca
      901 aaaagccggc tgtcatttat cacaacgtgg aacgcctcaa tgttacggtc gaagaacagc
      961 aggaatacat catggatttt ttccgtgatc gcaatatgta cacgtttttg gaactcgtcg
     1021 aggatatgag caagattgag gtggtggtca catttttagc attgctggat ctcgttaaaa
     1081 aaggagaaat cgcagcacgc caaaccacgc tttttgatga tatttggatt tataaagcat
     1141 ctgcatatac ggatgaaatt cccgatgagt tgcgcgctga agaatccgaa acggaaaaga
     1201 cgataccttc aaacgatgaa cctgttctca cgccgtccga ggcgcctgtg ttgaatgaag
     1261 tttcatcaaa cgatacaacg tttgcgatta atgaatccgg agacacggct ttggatacgt
     1321 cattaattga ggtcgccgca tcgcccgata gcgaattagc aaacgaccac attgatatgg
     1381 cgcaaaccga tggcgtggtg caagaagtcg aacaagccgc agttgaaatg agtattgaga
     1441 gtgttatcga agcagcgaaa accgatgcgg aaacggttga gttaaatgaa gaaatcctaa
     1501 ataacgaagc cttcgtttcg gctccacaaa ccgaagaaca accgcaaaca gaatatactg
     1561 atacatctga ggcgtttgct gattccgaaa atgatgaaat tataggcgct acagtcgaag
     1621 aaaatttaaa ttctctttca gatgaaaaca tttattcagt tgaagaaata cggtcaaatt
     1681 cgtcttcaga tgaaacaata tctgtatcgg tgaatgaatc aacggaagtt gatttttata
     1741 cccaagtacc atcggaagtt acttctgccg agcaaaatca gtctgatata agcgatgtta
     1801 aaacggaaaa tatgggttca catggtgagg atttttcaca cgaagaaacg gagttattgg
     1861 atcgtacaat agaaaatgtt tccgatgatg ggatcgcaac ggatttaacc gcctcaatat
     1921 ccgacgcgac tgttgatgga aatgtaactg atgatgctgt tttattgata tccacgacag
     1981 aagaccaaac gatagcatcg cccgttcccg tcgaacttat cagaacagta atcgccgaat
     2041 cgcaagtcaa tgtaaacaat tccgatctcg aatcggcgga tgaaatcacg ggcaatttag
     2101 ctctatcaga cactgaagat aaagtgattg tgccaacgaa tgagccgatc gtcggggagg
     2161 ctgaaacaaa tacgactgat attgatcaaa caccaccgca ggttagttca aacgaaatcg
     2221 gcgataatat taacgcagat caaagtgcat cagatacgat aatatcggtt gaggaaaacg
     2281 aacccgcatt aattcggtca aacgatgttt cgacgccgga tattcaagct attcacgaaa
     2341 atgaattaac ctccgaggat gtggttgaaa gtattacgtc ggaaccacca cagaacaccg
     2401 atcctgttca tatcatcgaa gcgatccaca atcaaaatta tgaaacgatt caaccggatt
     2461 tagtaaagcc gctcgtttca caagacgcgc caagaacgga tggcgagtct gttgtctcag
     2521 ctaaaatgga agatgatgtg agcaaaccct tggttgaaca acatgaaacc gccgatgcgt
     2581 ttcttagccg cacaacaaca aacgacgaaa aaccatcgct agatgaaacg gaaaaaccgt
     2641 ctattataac ggtatttgtt cgtaaaatag tatcttttgt caaacgcttt tttggaaaat
     2701 gataggttat taaagagatt atggaagaac agaatcaaaa cgaatccaac ttggaaccca
     2761 cggaagtaca gattgatacc gagacatcgg aggtccctgc ggatccgcgt attgtggacg
     2821 gattgccact gcaaaatgta gttgagtcca ttatttttgc atctaccgaa cctttgccga
     2881 tggatcgtct tttggagtgt attcaaaaag attacgttca ggcttcatta gttcgacaag
     2941 tgattgatga gatcaataag cattatcagg ccaacaattt accgttgcga gttatcaaaa
     3001 tcgccggcgg ttatcaatac gccacccatc cgcagtatga caaatgggtt agtcggcttt
     3061 tcaaatcaaa agcggagaaa aaattatccc aatcttcgct cgaagtgctg gctattgtgg
     3121 cgtatcgtca acctatttca cgtgcggaga ttgagcgtat ccgcggcgtg aatgccgatt
     3181 ggacattgcg ttcactgatg gaaaaaaacc tgattacggt cgtggggcgt gaagatgcgc
     3241 cgggaaaacc tttacttttc ggcacgacca agccgttctt ggagcatttc ggtttggatg
     3301 ccatcaatga acttcccaag ctcaaagaga tcgaagacat catcaaagaa gataaagaat
     3361 ttgcagagac gctggaattt gattttgccg ataaaaaggc tgcggaagct gcggctaaac
     3421 cggctgacga aacagcgaag aacgaaggac tgtctcagga attcgctgaa atagatacga
     3481 tacttaaaga aaataatttt aatcccgaag atcatttgaa gtgagaggcg atgaattttg
     3541 attatttaaa tgaccagact tggatcgtca atcaagacat tccatggaag catttgtcta
     3601 aagatctttc cgaagtgcgc attgcgatca tcactacggc tggactttat atgtggaaaa
     3661 cgcaaaaacc gttttctttt gaagatgaga taccggatgt ctcatttcgg gaaattccga
     3721 attccgttac ccaagaaacc gttcgtgtgt cccatcgtta catggatcta ttcgggtctg
     3781 ccggggtgga tatcaatagc gtgtttccta ttcatcgttt acaagagatg gtgtctgaaa
     3841 aacgcatcgg cggtcttgca gaaacccact acagcgtgat gggcgaagtc agagacccgc
     3901 gtttgtttat tgaagattgt atgcccaaaa tactgaaatt acttcaaaaa caattggtag
     3961 acgttgtttt ggtaacgccc ggggggccat tggggcatca aacagcagga atgatcgcgc
     4021 gatttctgga agagaacgat attactacga taaccatcgg cgcggtaaaa gccattgcgc
     4081 aaaacgttaa atcgccgcgt acgttattag tgcgttatcc tttcggcatg tatttcggtg
     4141 cgccatttga tactgttaat caaaaagaaa tagtgaatga gtgcttagtt catctgaaag
     4201 ccattcgcga acccggtgag gtgtccgaat tgagtgtacg gtggcaggat acttttaaaa
     4261 cagctttgca aatcaaaccg gagttaccga cgctgatgga caccggcgcc atcatcgcgg
     4321 aacccgtgat cgaaaagaaa aaaattgcag cggatgaatt ttaaattatg atgcgactta
     4381 ataaatttat ggctacggcg ggggttgcca gccgccgcag ttgtgatgaa atcatcggcg
     4441 aagggcgtgt caccgtcaac ggacaaacgg ttacggattt atcttatcag gtggatgagg
     4501 cgctagatcg tgtcgaagtg gacggtcagc ttctgtcgat caaagaaaaa gacgtgtaca
     4561 ttatcctgca caaaccgttg cggtatgtaa ccaccgtcaa agatgagcgg ggccgcgatt
     4621 cggtcgtgga tctagtcaaa attacggaac gtgtgtatcc cgtcggccga cttgattttg
     4681 atacgaccgg acttttgcta ttgaccaatg atggcgaatt agcttttcgt ttggctcatc
     4741 ccaagtttgg cattgaaaaa acatacatcg ctttgctcaa taaaaaaatc ggcgaatcgg
     4801 agttgcagca attgcgccgc ggcgtcgtgc tgattgacgg taaaacagca ccgtgtaaag
     4861 ccgaaaaatt gggaaaagag cttgtcaaaa ttaccatcca cgagggtaaa aacaaacaag
     4921 tcaagcgcat gtttcgtaaa ctgggctata aagtgaggga gctgcaccgc tcgcagtacg
     4981 gtcctataca gctcggtaaa ctcaaatacg gacagtggcg taatctcaaa ccggctgagg
     5041 tgttggcgct aaaaaaagcc acgggtttga taaaaaaata gtcatagtga agtgaaaaaa
     5101 ttactgatca cattagacgg tcccgcggga agcggaaaaa gtacgaccgc taaattatta
     5161 gctaagcggc ttggttattg ttatcttgat accggtgcca tgtaccgagc gataacattg
     5221 caagccatac atcacggtat ttttccgacc gatccggaaa aaatcattgc gatgttgccg
     5281 gacattacgt tgcatattga ttacaaagac ggacgccagc agacctttct caatggttgg
     5341 gatgtgtccg aaggtatccg atcacccgaa gtaacacgcc atgtcagcgc cgtttcggcg
     5401 atagccgagg tgcgaacttt tttgcggcaa cagcagcaac atatcggtaa ccagggcggt
//
LOCUS       DAOQNQ010000216         5459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_6024_length_5459_cov_5.692265, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000216 DAOQNQ010000000
VERSION     DAOQNQ010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6024_length_5459_cov_5.692265"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1738
                     /locus_tag="PK754_11880"
     CDS             <1..1738
                     /locus_tag="PK754_11880"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNB10250.1"
                     /translation="GKTTFDVVVSDKNTLMLTLDTRGLTIDSVKLDQGSEIANYVLDT
                     NIPNSDYANDSSAIQISSAQATMGQALRISVAPTTKKAHVYYHTGHEADALQWLAPAQ
                     TEGQKKPFLFTQSQPNLARTWFPCQDSPGIRMTYEARVQVPPGLMAVMSAESDTVLHA
                     DGIYHFRMTHAIPSYLVALAVGDFGFRPLGPRSGVYAEHTMLARAAAEFTDVEAMIAA
                     AEKLYGPYLWGRYDLIVLPPSFPYGGMENPMMTFATPTIVAGDRSLVSLVAHELAHSW
                     SGNLVTNATWGDIWINEGFTTYFERRIDEAVFGRSFSEMEAHLGHQDMQAALKELGTE
                     SPLTKLRLDLNDLDPDAGHSGIIYDKGYHFLRFMEEQIGRDRWDAFLRKYFATFAFQS
                     MTSERLMNYMRTELIRGDRALEEKLQLDQWILGTGLPSVCPVPQSDAFTKVEEEVKKW
                     KSGVPAKQLLTSSWQTQQWMHFLRLLPENMNTAQLTSLDEAFSFTRSGNAEIQFEWFQ
                     HVITHKYTPAYARLEHYLTHIGRRKLVFPLYRKMASDPEMRIMAKRIYAVARRGYHPV
                     TYGSIDEILLKK"
     gene            complement(1749..3062)
                     /locus_tag="PK754_11885"
     CDS             complement(1749..3062)
                     /locus_tag="PK754_11885"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNB10251.1"
                     /translation="MIFTERTSIVLVGAALAFILGAYMPALIFAGWVLLLLLVAGLLI
                     ELRSFPVRSSFSVNRSYPKKMAVGVRNTVVLEIQSQSSRSWSIRIHDEPPDIWQMREV
                     NFQDTLPPHRALRWMYDVYPMHRGVFSFYTTTIEVKGIFFDLVRKRYAFPSEQTVQVY
                     PDFRTHTQPALLSVNKRNILTSQRSVRRYGAGTEFESLREYTPDDEYRKINWSASARS
                     GKWMANQFQMERAQHIMLLIDTGRLMGSEAFGLSKLDHAVNAAARMTQVCIAHGDAVG
                     LMLFGSAVHSFLLPKKNTSQRALIAESLYTARLERTESDYTTAFQWLQRRQRHRALVL
                     VFTEIVDRYSSQILLGSMASLYPKHLPVFILMKDKALTEVLQRPVMHSDSAFEKGVTA
                     ELLREREEAIAFARSRGALVIDAMPDHFHSAVINLYLDIKARGRL"
     gene            3173..3577
                     /locus_tag="PK754_11890"
     CDS             3173..3577
                     /locus_tag="PK754_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10252.1"
                     /translation="MKRKLSRAQFLKLFSGVFLFFGMSQWMLSCASEKKSDDDDGDNG
                     GGGTDPKPDCINAGTKISISGNHGHTLSVSKADVAAGTEKTYNITGSSAHSHSVTITA
                     EHFAQLAANTSIVITSTPSGHSHQVTVSCALA"
     gene            complement(3636..3722)
                     /locus_tag="PK754_11895"
     tRNA            complement(3636..3722)
                     /locus_tag="PK754_11895"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3686..3688),aa:Ser,seq:cga)
     gene            3963..4940
                     /locus_tag="PK754_11900"
     CDS             3963..4940
                     /locus_tag="PK754_11900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNB10253.1"
                     /translation="MKKSTITIVKKFRRFIIWWIEFLGWLYKTPVIYYLQQLQQRIFD
                     RHKLTTNAAAISFFFLLSIIPLLFIVLSIIASFVQSPEEAQAYISETLTNRVPDTARD
                     FVLQLIQKSNLTESVTSILENKGWISLVSIGSLLWTSSGAFAAIEDAMSTIFGVRGRN
                     YFVSRLVEMGMVVLIGCLFLLSSLISGIIHWLQENHITFFQIDFSNLPYVWDIITNAL
                     PYVLIVLTFYVTYKILPKTEIYDRAAMLGALVASVLLELTLYGFSYYVQNFARYNVFY
                     GSVAGVVISVFTIYLASIILLVGAEITEIANTRLENERELAQTIREFES"
     gene            4937..>5459
                     /locus_tag="PK754_11905"
     CDS             4937..>5459
                     /locus_tag="PK754_11905"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNB10254.1"
                     /translation="MTSSSLPVLHPRYDLIEKIGEGGVGIVYRAWDKIDRSTVVIKQA
                     PASTINDTLRDAYWREFEWMFRAHHPGMMKALAWEKSEDGGFFFTMQLAAGIDLFQKI
                     KDRGFWTKKRFDSFIRQIVSALSYVHAHGMVHGDIKPENILIESAGEDCKVTLIDFGF
                     IHAWSNTPVSRDHQ"
BASE COUNT         1435 a         1170 c         1355 g         1499 t
ORIGIN      
        1 aggcaaaact acctttgatg tggtggtgtc cgataaaaac acgttgatgt tgaccttaga
       61 tacgcgcggt ctgacgattg attccgtcaa gctggatcag ggatccgaga tagcgaatta
      121 tgtattggat acaaatattc cgaattccga ttatgcgaac gatagttcgg ccatacaaat
      181 atcgtccgca caagccacga tgggccaagc cctgcgcata tccgtagcgc ctacaactaa
      241 aaaagcgcac gtatattatc atacgggtca tgaagccgac gctttacaat ggttggcgcc
      301 cgcgcaaacg gaaggacaaa agaagccttt tctatttacg caatcgcaac cgaatctagc
      361 aaggacatgg tttccctgtc aggatagtcc cggcatacgc atgacgtacg aagcgcgcgt
      421 tcaggtaccg cccggtttga tggccgtgat gagcgccgaa agtgataccg ttttgcatgc
      481 cgacggcatc tatcattttc gaatgacaca cgccataccg tcgtatttgg tggcgttggc
      541 tgtcggtgat tttggttttc gacccttggg gcctcgtagc ggcgtgtatg cggaacatac
      601 gatgctggcg cgtgcggctg cagaatttac cgatgtcgaa gcgatgatcg ccgcggccga
      661 aaagttatac gggccttatc tgtgggggcg ttacgatctg atcgtactcc cgccgagttt
      721 cccgtacggc ggcatggaaa atccgatgat gacttttgct acgccgacga tcgttgcagg
      781 ggatcgttcg ctggtgtctc tggttgcgca cgaattagca cactcatggt ccggtaatct
      841 tgtcaccaat gcgacatggg gtgatatctg gatcaatgag ggatttacga cgtattttga
      901 gcgacgtatt gatgaagccg tatttggccg ctcattttcc gaaatggaag cgcatctggg
      961 gcatcaggat atgcaagcgg cattaaaaga attgggtacg gaaagtccac taactaaact
     1021 gcgattggat ctgaatgatc tggacccgga tgccggtcat tccggaatta tttatgataa
     1081 agggtatcac tttttgcgtt ttatggagga gcagatcggt cgtgatagat gggatgcttt
     1141 tctcaggaag tatttcgcca catttgcctt tcaatccatg acttcggaac gcctgatgaa
     1201 ttatatgcgc acggagctca ttcgcgggga tcgtgcgttg gaagaaaagc tccaactgga
     1261 tcagtggata ttgggtacag gtttaccgtc ggtttgtccg gtgccgcaat cggatgcttt
     1321 tactaaagtc gaagaagaag taaaaaaatg gaagtcgggt gtgccggcaa aacaactgtt
     1381 gacctcatcg tggcagaccc aacagtggat gcacttttta agattgttgc cggagaatat
     1441 gaatacagcg caactcacaa gtttggatga agcttttagt tttacacgtt cgggtaatgc
     1501 agaaatacaa tttgaatggt tccaacatgt gataacgcat aagtatacac ccgcttacgc
     1561 gcgtctggaa cactatctta cgcatatcgg gcggcgcaaa ttagtgtttc cattgtaccg
     1621 taagatggca tccgatccgg agatgcgcat catggccaag cgtatttatg ctgtagcgcg
     1681 acggggttat cacccggtga cgtatggttc cattgatgaa attttgttaa aaaagtagag
     1741 aaaaattttc acaaacgtcc gcgggctttg atgtcgagat agaggttgat cacggcggaa
     1801 tgaaaatgat ccggcatagc atcaattacg agcgcgccgc gtgagcgcgc aaaggcgatg
     1861 gcttcttcac gttcgcgcaa aagttcagcc gttacgcctt tttcaaaagc cgagtcacta
     1921 tgcattaccg gtcgctggag cacctccgtc agcgctttgt ctttcatcaa aatgaatacg
     1981 ggcagatgtt tagggtacag tgaagccata ctgccgagca gaatttgaga agaatagcgg
     2041 tccacgattt ctgtaaaaac taaaactaga gcacggtgtc tttggcgtcg ctgcagccat
     2101 tggaaagctg tcgtataatc cgattcggtt cgttccagtc gagcggtata tagactttcg
     2161 gctatgaggg ctcgttggga tgtgtttttt ttaggtaaaa gaaaagaatg aacggcggaa
     2221 ccgaataaca tcaggccgac cgcgtcaccg tgtgcgatac atacttgggt catgcgcgcc
     2281 gcggcattga cggcgtgatc gagtttgctg agaccaaacg cctcgctgcc catgaggcgc
     2341 ccggtgtcta tcaagagcat gatgtgttgt gcgcgttcca tctggaattg attggccatc
     2401 catttcccgc tgcgcgcgga ggccgaccaa tttattttgc ggtactcatc atcgggggta
     2461 tattccctca agctttcaaa ttcggttcct gctccgtaac gccggactga acgctgggaa
     2521 gtcaaaatgt ttcgtttgtt taccgagagc aacgccggct gggtatgcgt acgaaaatcg
     2581 gggtatactt gaacagtctg ttccgatgga aacgcataac gtttgcgtac aagatcgaaa
     2641 aaaatacctt ttacttcgat cgtagtagta taaaatgaga aaacaccgcg atgcatcgga
     2701 taaacatcat acatccatcg gagtgcgcgg tgcggtggca acgtatcttg aaaattcact
     2761 tcacgcattt gccatatatc cggcggttca tcgtgtattc ggatagacca agaccgcgag
     2821 gattggcttt gaatttccag tacaacggta tttcgaacgc ccacggccat tttttttgga
     2881 taagatcggt tcacggaaaa cgatgatcgt accgggaacg aacgcaattc gatgagcaat
     2941 ccggcaacga gtaatagcag cagcacccat ccggcgaata tcaatgccgg catataggcg
     3001 cctaggataa aagccaaagc tgcgccgact aaaacgatgg aagttctttc cgtaaaaatc
     3061 atagcctaag ttccacgaga taaaggcgaa aatcaaataa aaaatattgc cctgacgaga
     3121 gtatgccatt agattatata atctgtccta tcattgtaga aaggaaaatc gtatgaagcg
     3181 taagttatcc cgtgcacagt ttcttaaact tttctccggt gtttttttgt tttttggtat
     3241 gagccaatgg atgttatcct gcgcgtcaga aaaaaaatcc gatgatgacg atggcgacaa
     3301 tggtggcggg ggtacggatc cgaagccgga ttgtatcaat gcaggtacca aaatttcaat
     3361 aagcggaaat cacgggcata cgttatccgt atctaaagcg gatgtggcag caggcacgga
     3421 gaaaacatac aatatcacag gttcatcggc acatagccat tccgtgacga taacggcaga
     3481 acattttgcg cagcttgcag cgaatacttc gattgttatt accagtacgc cgagcggcca
     3541 tagtcatcag gtaacggtaa gttgtgcttt ggcttgagaa agtcaaacta aataaaacaa
     3601 taaaacccgg ttttttagcc gggttttatt gtttgcggag agggtgagat tcgaactcac
     3661 gaaacccttg cgggtttacc ggttttcgag accggctcct tcaaccactc ggacacctct
     3721 ccaaatccta tctgctataa ttgctttttt taaaagcggg taacaatact gaatcatgct
     3781 ctgaaaatca aatctttttt agataaattt gaatttccga tatgcgaaat agtgtgttta
     3841 ttgctacgat atcaatgtat ttactttcat ggcatgcaaa gtatgtatga cgaaatttcc
     3901 gtcaaaactt gcatacgtaa ataaatgttt ttactttgtt ttaaataaaa attagtgatt
     3961 ccatgaaaaa gtcgacgata acgattgtca aaaaatttcg ccgttttatt atttggtgga
     4021 tcgaattttt gggttggtta tacaaaacgc ccgtgattta ttatttacag caactgcagc
     4081 aacgtatttt tgatcgtcat aaacttacaa ccaatgcagc ggccatttcg tttttctttt
     4141 tattatctat tatccctttg ctttttattg tgctttcgat tatcgcatcg tttgtgcaat
     4201 cgcctgaaga agcacaagcc tatatttcgg aaactttgac gaaccgggtt ccggacacgg
     4261 cgcgcgattt tgtgttacaa ttgattcaga aaagcaatct taccgaatcc gtcacatcta
     4321 tacttgagaa taaaggctgg atcagccttg tcagtattgg ttcgctgctt tggacaagca
     4381 gcggtgcgtt tgccgcgatc gaagatgcaa tgtccacgat attcggtgta cggggccgaa
     4441 attactttgt cagccgttta gtcgaaatgg gtatggtcgt tctcatcggc tgtctctttc
     4501 tgctttcgag cctgatcagc gggattattc actggctaca ggaaaatcat atcacttttt
     4561 ttcaaattga tttttcaaac ctgccgtacg tttgggatat tattacaaac gccttgccgt
     4621 atgtgctcat cgtactcacg ttttatgtca cgtacaaaat tttacccaaa acggaaatct
     4681 atgatcgtgc ggcgatgctc ggtgcgctgg tagcgagcgt gctgctggaa ttgacgcttt
     4741 acggttttag ttattatgtg caaaattttg caagatataa tgtgttttac ggatccgttg
     4801 ccggtgtggt gatttctgtt tttacgattt atttggcttc cattatttta cttgtcggtg
     4861 cggaaataac cgaaattgcc aatacgcgtt tggagaacga gcgcgaattg gcgcagacca
     4921 taagggagtt tgagtcatga cttcatcctc cctacccgta cttcatccgc gttacgatct
     4981 gattgaaaaa atcggtgaag gcggggtggg tatcgtctat agagcgtggg ataaaatcga
     5041 tcgctcgacg gtggttatca aacaggcgcc ggcgtcaacg attaatgata ccttgcggga
     5101 tgcgtattgg cgtgaatttg aatggatgtt tcgtgcacat catcccggca tgatgaaggc
     5161 gctggcttgg gagaaatccg aggacggagg tttcttcttt acgatgcaac tggcagccgg
     5221 gatcgatctg tttcaaaaaa taaaagatcg cggattttgg actaaaaaaa gattcgatag
     5281 ttttatccga caaattgttt ccgctttgtc ctatgtgcat gcgcacggca tggtgcacgg
     5341 cgacatcaaa ccggaaaaca tcctgatcga atcggccggc gaagattgca aggttacatt
     5401 gatagacttt ggattcattc acgcctggtc caataccccc gtttcacgtg accatcaag
//
LOCUS       DAOQNQ010000217         5453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_6030_length_5453_cov_6.546128, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000217 DAOQNQ010000000
VERSION     DAOQNQ010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6030_length_5453_cov_6.546128"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PK754_11910"
     CDS             <1..458
                     /locus_tag="PK754_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HNB10255.1"
                     /translation="IKNVRFTIDSTRVGQHTDYEKLTLEVTTDGSITPDDALTIAARL
                     FRDHIQLFINFEMEDENADGEVEDQEAARIRQLLKMSVDELELSVRSHNCLRAAGIKT
                     IGDLVSKNESEMLKYRNFGRKSLTELTKILEERNMSFGMDVSKYYNGEE"
     gene            483..1049
                     /gene="rplQ"
                     /locus_tag="PK754_11915"
     CDS             483..1049
                     /gene="rplQ"
                     /locus_tag="PK754_11915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNB10256.1"
                     /translation="MRHGKKQIKLSKTASHRQALIKSLCNELFRHKRIVTTISKAKAA
                     RMTAERLIMFAKKGDLAARRILISRLGNARLVTAHKHVTDKKELSKTVIHELINEIAP
                     KMKELDAQRKAKNASYTGGGYTRVLRLGLRKGDATEMAVLELVGYETASIDKQNKAIE
                     AKEAKAQRAKSLAERIKAKKEEAQKESK"
     gene            1069..1395
                     /locus_tag="PK754_11920"
     CDS             1069..1395
                     /locus_tag="PK754_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10257.1"
                     /translation="MPRTKKEAADEKETKAPKKKAAAPRKKKEEVKTEAKATVSKKTE
                     AASEVLLPKIQCFAPGKKTICAHLMTSGHAHQCAAKRLPNGKLSTVRSGKKRRCEFYQ
                     ETTYNG"
     gene            1525..2655
                     /locus_tag="PK754_11925"
     CDS             1525..2655
                     /locus_tag="PK754_11925"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNB10258.1"
                     /translation="MKLLSKYLFIGFVLLSYGLPSNVTVAQEKARIKAIWVVRDILKS
                     KIEIDRMLSFCDQNGITDVFLQVRGRGDAYYNSDFVVKAEGLDPAFDPLQYVIEKNRL
                     KKIKIHLWLNVFYLWSSEKNPVHPEHLFYTQPDWAAVNAQGQSMSVEGTKKLIAKRLE
                     GVFISPAIQEFQDYFSAVVKELVSKYPVDGIHFDYIRYPEADYDYSTSMRSRFILEYH
                     WDPLAEALNAWQDSIWTRFRKRIIEDYVACIYTQIKKADSSIAVSAAVWASQDDAQNR
                     VLQDWPEWIKSNCLDFAVPMNYATDNGEFEKRIQDAVIKLGASDTQKIIMGVSLYNQP
                     VKSAEEKLRICRKYGFAGVSFFSYETLRTQKEYKTLVKNFNH"
     gene            2667..4556
                     /locus_tag="PK754_11930"
     CDS             2667..4556
                     /locus_tag="PK754_11930"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNB10259.1"
                     /translation="MRIINSLFVITALIRCDLTAQEVDADSLLRKLRVYPIRDSARVE
                     ILTDICAALTFKEPDSVLIYAEEALIISKEIGYEYGIATAINCRGMAYYQKGDLTKAM
                     ENYLQASDYVKQHRIRNPRLDYADFVILNNIAIVYNNKQLVDEALAIHFQQIELLPLI
                     SANPIHYAALYTNIGGCYGNKKMFAEGIRWFEKVDSIAKVYNIPYGTSIAMLNIAANY
                     NNMKLYSKAITSFRQSLTIAQALRIADTESACHNGLGVAYSETGRWAEAVLSFEAGAE
                     VAKRSGLLSNAMTAYQGLARAAVQTGDFRKAYEAQMQYQQINDSLLSIEKTKSLQELI
                     TRYETEKKEITIREQQERLKVQSLESDRKNILIVGIILLLAVVGGASFFYIQTLQAHK
                     KNEILSMQMIAAEKSKAMEVAIAQSELKGLKSQMNPHFIYNVLNSIQSFVYANQKNEA
                     SENLSKFSDLMRKTLENSGRQWITLHEETEYLSRYLELEALCADHAFQYEIVVDPGLD
                     PYTVEVPAMVIQPFAENAIKHGLLHKSGSKKLSIRFEKNLSGNDLRVVIEDNGIGRAA
                     SAEINARNGKKPRSFATQATAERIALLSQLQSRSIVCQFTDEPSEHGAEGTRVDIVFP
                     LEDRA"
     gene            4553..5305
                     /locus_tag="PK754_11935"
     CDS             4553..5305
                     /locus_tag="PK754_11935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNB10260.1"
                     /translation="MSRLRIIIIDDAVKARETLRLMISENMTDIEIVGEAGTLPDGVK
                     LIHKTNPDVVLLDIEMPGHSGLEILDFFSPERIHFKIIFVTAYAEHAVRAFELSAVDY
                     ILKPVSPNRLKQALDKVTLGQINAEQYQVLKEQTAGTWNKIALKTGDGIVFIPTDQII
                     YLKAEGAYTKFILMKQETILVSKNLAEYEKLQSAGPFVRAGRSIILNLSRIKKIGKAS
                     DGSVTMDNGDTLAIGDETYKQLVEAIKTIKLG"
BASE COUNT         1711 a         1120 c         1226 g         1396 t
ORIGIN      
        1 cgatcaaaaa tgtacgtttt actatcgaca gtacacgcgt cggtcagcat accgactatg
       61 aaaaactgac cctcgaagtc acgacggacg gcagcattac accggacgat gcgctgacta
      121 ttgccgcacg cttattccgc gaccatattc aacttttcat taactttgaa atggaagatg
      181 aaaatgccga cggtgaagtc gaagatcagg aagccgcgcg cattcgtcag ctccttaaga
      241 tgagcgttga tgaactggaa ttgtcggtgc gttcacataa ttgtttacgt gcagcgggca
      301 tcaagactat cggtgatctt gtcagcaaaa acgaatcgga aatgcttaaa tatcgcaatt
      361 ttggtcgtaa gtcgctgacc gagttaacca agattctcga agaacgcaat atgagtttcg
      421 gtatggatgt cagcaaatac tacaacggtg aagaataatt ttaatttggc attagaggaa
      481 cgatgagaca cggtaagaaa caaataaagc tgagcaaaac ggcaagtcac cgtcaggctc
      541 tgatcaaaag tttgtgcaac gaacttttcc gtcataaacg tattgtgacg actatttcca
      601 aggcgaaagc ggctcgtatg acggccgaac gcttgatcat gtttgccaaa aaaggtgatc
      661 tggcagcgcg tcgtatattg atcagtcgcc tcggtaacgc ccgtttggta acggcgcata
      721 aacatgttac cgataaaaaa gaactttcca agaccgtaat tcatgagctc atcaacgaaa
      781 tcgcaccgaa gatgaaggaa ttggatgctc agcgtaaagc taaaaatgca tcttataccg
      841 gcggtggcta tacacgcgtt ctgcgtttgg gtctgcgtaa aggtgatgcg acggaaatgg
      901 ctgttttgga attagtcggt tatgaaacgg cttccattga taaacaaaac aaagccatcg
      961 aagccaagga agctaaagct caacgcgcca aatcgctggc tgaacgtatc aaagcaaaaa
     1021 aagaagaagc tcaaaaagaa tccaagtaag ttttagatag aggtaaaaat gccccgtacc
     1081 aaaaaagaag ctgcggacga gaaagaaacg aaagccccga agaaaaaagc ggcggctccg
     1141 cgtaagaaaa aagaagaagt caaaacggaa gctaaagcta ccgtaagtaa aaaaaccgaa
     1201 gcggctagcg aagtattatt gcctaaaatt caatgctttg caccgggcaa aaaaactatc
     1261 tgcgcgcatc taatgacctc cggtcatgca caccagtgcg cggctaaacg tttgcctaac
     1321 ggaaaacttt caaccgtgcg ttcaggtaaa aagcgtcgtt gtgagtttta tcaggaaacg
     1381 acctacaacg gttaattaga tctcggaaat ttttataagg cgttcatatt tatgaacgcc
     1441 ttttttattt ttgtagtatg taaaatgcat tccaatatgt ataataatcg atcattggaa
     1501 attaaccaaa tagccgaata gcgaatgaaa ttattaagta aatatttatt tattggcttt
     1561 gtgctattgt catatggatt gccgtccaac gtaaccgtag cacaagagaa ggcgagaata
     1621 aaagcaatat gggtagtccg agatatttta aaatctaaga ttgaaattga tcgcatgttg
     1681 tccttttgtg atcaaaacgg aatcacggat gtttttttgc aagtacgcgg tcgcggtgat
     1741 gcatattata attccgattt tgtggttaaa gccgaaggcc ttgacccggc gtttgatcca
     1801 ttgcagtacg tgatcgaaaa gaacagatta aaaaaaataa aaattcattt atggctgaat
     1861 gtattttatt tatggtcatc cgaaaaaaat ccggtccatc ccgaacatct tttttacaca
     1921 caacctgatt gggccgctgt caatgcacaa ggacagtcca tgtcggttga aggaacaaaa
     1981 aaactaattg ccaagcgact cgaaggcgtt tttatctcgc ccgcaatcca ggagttccaa
     2041 gattattttt cagctgtagt aaaagaattg gtatctaagt atccagttga cggcattcat
     2101 tttgactata tccgttaccc tgaggcggac tatgattatt ctacatccat gcgcagtcgc
     2161 tttattttag aatatcattg ggatccttta gccgaagcat taaatgcctg gcaggacagt
     2221 atttggacgc gctttaggaa aagaattatc gaagattatg ttgcctgcat atatacacaa
     2281 atcaaaaaag ccgattcgtc cattgcggtt tcagcggccg tatgggcgtc acaagacgat
     2341 gcccaaaatc gtgttcttca agattggccg gaatggataa agtcaaactg tttagatttt
     2401 gctgtgccta tgaattatgc aacggataac ggagaatttg aaaaacgtat ccaggatgcg
     2461 gtaataaaat taggcgcatc cgatacccaa aaaatcatta tgggtgtttc tttgtataat
     2521 cagccggtaa aaagcgccga agaaaaattg agaatatgtc gtaagtatgg ctttgccggg
     2581 gtatcttttt tttcttatga gacactccgt acgcagaaag agtataaaac gcttgtcaag
     2641 aattttaatc attaacttat aacatcatgc gcatcattaa ttccttattc gttattacgg
     2701 ctttaatccg gtgtgatctt actgcacaag aagttgatgc cgattctttg ttgcgcaaac
     2761 tgcgcgttta tcctatccgt gattcagcac gcgtggaaat tttgacagat atctgtgccg
     2821 cgttgacatt caaagagccg gacagcgttt tgatatacgc tgaagaggcg ctgatcattt
     2881 ccaaagaaat cggatacgag tacggaattg caacagccat caactgccgc ggtatggcat
     2941 actaccaaaa aggcgacctg acgaaagcga tggagaatta tttgcaagcg tcggattatg
     3001 tcaaacagca tcgcattcgt aacccgcgtc tggattacgc cgattttgtt atcctaaata
     3061 atatagcaat cgtctacaac aataaacaac ttgtagacga agcgttggct attcacttcc
     3121 aacagatcga acttttgccg ctgattagcg ctaaccctat tcattatgcg gcattgtata
     3181 ccaatatcgg cgggtgttat ggtaataaga aaatgtttgc cgaaggtatc cgttggtttg
     3241 aaaaagttga ttcgatcgcc aaggtatata acatacctta tggaacatcc atcgccatgc
     3301 tcaatatcgc agcgaattat aataacatga aattatacag caaggcgatt acgagctttc
     3361 gtcaatccct caccatagcg caagcgttgc gtatagcgga tacagaatcg gcatgtcata
     3421 acggattggg tgtcgcctac agcgaaacgg gccgttgggc ggaagctgta ctaagctttg
     3481 aagccggtgc tgaggtagcc aagcgatccg gattattgag taatgcgatg acggcgtatc
     3541 aaggtttggc acgtgccgcg gtacaaacgg gcgattttcg aaaggcctat gaggcgcaga
     3601 tgcagtatca gcaaatcaat gacagtcttt tgagtataga aaaaacaaaa tccctgcaag
     3661 agctcatcac gcgctatgaa acagagaaaa aagaaataac tattcgcgag cagcaagagc
     3721 gcctgaaagt ccagtcgctg gaaagcgatc gaaaaaatat actgatcgta ggaattatat
     3781 tactgcttgc cgtcgtcgga ggcgcttcat ttttttatat tcaaacccta caggcccaca
     3841 agaaaaatga aatactcagt atgcagatga tcgctgccga aaaaagtaaa gcaatggaag
     3901 tggctatagc tcaatccgaa ttgaagggac ttaaatccca aatgaatccg cacttcatat
     3961 acaacgtact caattcgata cagagtttcg tttatgctaa tcaaaagaac gaagccagtg
     4021 aaaatctcag taaattttcc gatctgatgc gtaaaacatt ggagaacagc ggccgccaat
     4081 ggataacact gcacgaggag accgaatatc tttcccgtta tttggagctg gaagcgcttt
     4141 gtgcggatca tgcttttcag tatgagatcg ttgttgatcc gggattagat ccttatacag
     4201 tcgaagttcc ggctatggtc atacaaccct ttgccgaaaa tgcgatcaaa cacggcttgc
     4261 tgcacaagtc gggttccaaa aaactcagca ttcgttttga aaagaattta tcgggaaacg
     4321 atctgcgtgt tgtgatcgaa gataacggaa tcggtcgcgc cgcgtcagcg gaaatcaatg
     4381 cgcgtaacgg caaaaagccg cgctcatttg ccacgcaagc cacggcggaa cgcatcgcgt
     4441 tattgagtca actgcaatcg cggtccattg tgtgtcaatt tactgacgag ccgtcggaac
     4501 atggagccga aggtacacgc gttgatatag tctttccttt ggaggacaga gcatgagtcg
     4561 cctgcggata atcatcatag atgatgccgt caaagcgcgt gaaacattgc gccttatgat
     4621 atcggaaaac atgaccgata ttgaaattgt cggtgaagcg ggtacgctgc cggacggtgt
     4681 aaagcttatt cacaaaacga atccggacgt ggtattgctg gatatcgaaa tgcccggcca
     4741 cagcggtcta gagatactcg attttttttc tcctgaacgg attcatttta aaataatttt
     4801 tgtcacggcg tatgccgaac atgccgtacg tgcatttgaa ctttcggccg tggattatat
     4861 tttaaagccg gtaagtccca atcgcctgaa gcaagcactg gataaagtaa ccctcggtca
     4921 aatcaacgca gaacagtatc aggtactcaa agaacaaaca gcaggaacat ggaataaaat
     4981 cgcattaaaa accggtgacg gtattgtttt tataccgacc gatcagatca tatatctcaa
     5041 agcagaaggc gcatacacca aatttatatt aatgaaacaa gagactattt tagtttccaa
     5101 aaatctggcc gaatatgaaa aactgcaatc ggcaggccct tttgtccgtg caggccgttc
     5161 catcatcctc aatctgtcgc gtatcaaaaa aataggcaaa gcttcggatg gcagtgttac
     5221 gatggataac ggcgatacac tggcgatcgg cgatgaaacg tataaacaat tagtcgaagc
     5281 catcaaaacc atcaaattgg gttaaacgtc ctcggaagaa atctgatttg acgcattaac
     5341 ctcttcgtac aatcaaattg ataatctccg tttatgcggc tatattcaac attacttatt
     5401 aacttatgct caaaacgagt ctgaaagggc tatgaagcgc attgtattaa tat
//
LOCUS       DAOQNQ010000218         5440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_6055_length_5440_cov_4.789786, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000218 DAOQNQ010000000
VERSION     DAOQNQ010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6055_length_5440_cov_4.789786"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PK754_11940"
     CDS             <1..459
                     /locus_tag="PK754_11940"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNB10261.1"
                     /translation="LHRSFNQINEFSSNVSHELRTPLTILRGNIEVALSRERTPEAYV
                     EVLSDLLEEVLSVIQIVDGLLLLARGDARQIVTEKQRWLVSDIISEFSKDWEAIASLR
                     HQTVKLSPCPPLYVQGDKNLLSQLFLNIITNASKYSPPDSVIEIEINEIV"
     gene            484..708
                     /locus_tag="PK754_11945"
     CDS             484..708
                     /locus_tag="PK754_11945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB10262.1"
                     /translation="MVRDHGIGIPAEDTPKVFERFYRVNKDRARESGGTGLGLAIVRM
                     IVELHNGQIELESKQGHGTTISIFLPEASS"
     gene            900..2204
                     /locus_tag="PK754_11950"
     CDS             900..2204
                     /locus_tag="PK754_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNB10263.1"
                     /translation="MGWREDYKRKTVTAEEAVLAIKPGDRVFTSGNAATSYVLLSALA
                     KRKDELTDVEVTHVLLLGDDPLSKPEMAGHFRHNSLFVGPADRDAVNDGRADYIPVFL
                     YEIPKLFASGEKKLDVAIIQTSMPDDHGYMSLGVETLATMAAIRAAKIVLAQVNENMP
                     RTLGDCFVHVSKIHKLVEVHEDLPELPRPQITDTEKRIGQYVAQLVDNGATLQLGIGG
                     IPDAVLASLADKHDLGIHTEMVSDGVIQAIESGIVTGSKKTLHPGKVIGTFALGSKHL
                     YNYIDNNPMFEFRPSDYTNDPFIISRNENMVAVNSAIEVDITGQICADSIGTKIFSGF
                     GGQVDFIRGAAYAKGGKPIIALPATAQGGKTSRIAPALKSGAGVVTTRADVHYIVTEF
                     GIAYLHGKNLRERAEALIAIAHPDFREELSRAAHERFKYNSK"
     gene            2219..3607
                     /gene="vorB"
                     /locus_tag="PK754_11955"
     CDS             2219..3607
                     /gene="vorB"
                     /locus_tag="PK754_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNB10264.1"
                     /translation="MKARPKPFVMPEFCKGCERCVEACAKGCITPGHEVHPVSGMIPI
                     ELHLEECSGCGLCISACPEPFGLRPLENEPFVEMVDPFSLNGPRPTSSQPAEDMAARL
                     IPLPPSEPLVIKGTYASAIGALLAGCRHFYGYPITPSTEGSELMAKLLPELNGVFVQA
                     VSEVAAVNYLYGCASAGLPCMTFTSSPGFSLMLEGISYMIGAELPAVFVNVMRGGPGL
                     GNIGPEQADIKLVCRGLGHGNTHAIVLAPSSPQEMMDLTMQAFQLAFKYRNPVIVVGD
                     GYMGQMTGKVELPDHMIKPGLPAWAVWGDEAHRGNLISSIQLSEQDLEKHNLMINQKY
                     AHMTQDEQQADLFRCDDADVLIVAANTPARMAKGAVQELRRQGMRVGLFRPITLWPFP
                     IDRLKPLLYHVKQIICVEAGNGQLEDELRLAMSKAELPMPRVEHVRHFGGVLPQTHEI
                     VEKVLSMEEVIA"
     gene            3604..5052
                     /locus_tag="PK754_11960"
     CDS             3604..5052
                     /locus_tag="PK754_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNB10265.1"
                     /translation="MKTSVFYENFERHAHGEGLKGHSTHYCPGCGHGLAHKFLAETID
                     ELGIQDRTIAISPVGCSVFMYYYLDVGNTQAAHGRAPSVAIAHKMANPNSIVISYQGD
                     GDLASIGLAEIMQAAQLGIPITVIFVNNAIYGMTGGQMAPTTLMGQKTSTSPHGRDTM
                     MGMPLRMSEILAQMDGPVYIERVALYDSKQRVHAKKAIKKALQLQVEGRGFAFVEILS
                     ECPTHLKMTPSEAEAWVEKEMTAVFPLGVKKDVTREPWFMLEEPNFDAAKMMEKINAS
                     SQKEKRFCTAFPHHITPHDIALKLAGSGGDGAQTAAMLITRAGINEGFDATHIPSYGP
                     ESRGGTSYADVHIAEEEVLSPASPHPNVLIAFNAPSLLKFGPQVASNGIILYDSSVIP
                     NPPNFNHGVRMYGIPFTQIGMDLGRAMVKNIVALGALQAATQMFPKDTFLTAIRQALK
                     DKCAMIPLNEEAFDWGIRAFHGEIAKPGEPVV"
BASE COUNT         1377 a         1419 c         1338 g         1306 t
ORIGIN      
        1 cttcatcgct cttttaatca gatcaacgaa ttttcgtcca atgtctctca cgaattgcga
       61 acaccgctga ctattttgcg cggtaatatc gaagtggcgc tctcccgtga acgaacaccc
      121 gaagcctacg tcgaagtact gtctgatctt ttggaagaag tgttatccgt cattcagatc
      181 gtggacggtt tgttactgct ggcgcgtggt gatgcacgtc agatcgtaac cgaaaaacaa
      241 cgctggcttg tcagcgacat catcagtgaa ttttctaaag actgggaagc catcgcttct
      301 ttgcgccatc agacggtaaa attgagccca tgcccgccct tatacgtcca aggtgataaa
      361 aatctactga gccagttatt tctcaatatt atcactaatg cctccaaata ttcgccgccg
      421 gattccgtga tcgaaatcga aatcaacgag atcgtataat cagcccaatc gtacgttaaa
      481 atcatggttc gagatcacgg aataggtatt ccggccgaag atacaccgaa agtttttgaa
      541 cggttctatc gcgtcaataa agaccgtgca cgggaaagcg gcgggaccgg actcggactg
      601 gctattgtac gcatgatcgt tgaattgcat aacggacaaa tcgaactgga gagcaaacaa
      661 gggcacggaa ctacaatttc gatttttttg cccgaagcat catcataaga ggctttttta
      721 tacttccgat gatgaattct tgtattttca actaagttcc tattttttag catttctcat
      781 ttccgcacct gataaatatc acccgatcat ttgattccca tcctaacgcg ccgcaaacaa
      841 atactgtacc ttatatcagt cctgctattt gacaatgatt ttatggagtt tacacctata
      901 tgggatggcg tgaagattat aaacgcaaaa cggtaactgc ggaagaggcc gtattggcca
      961 tcaaacccgg cgatcgtgta tttacgagcg gtaatgctgc cacatcgtat gtcctgctca
     1021 gtgcgcttgc aaaacgtaaa gacgaactta ccgatgttga agtaacccac gtgcttttac
     1081 ttggcgatga cccgctttcc aaaccggaaa tggccgggca ttttcgccat aattcattat
     1141 ttgtaggccc ggccgatcgt gatgccgtca atgacggacg cgccgactat atccccgttt
     1201 tcctctacga aattcccaaa ctttttgctt cgggcgaaaa aaaactcgac gtagctatca
     1261 ttcagacctc catgccggat gatcacggtt acatgagtct cggtgtggaa acgctggcta
     1321 cgatggccgc gatacgtgcc gctaaaatcg tactcgctca ggtcaatgaa aacatgccgc
     1381 gaacgctcgg cgattgtttt gtgcacgttt ccaaaattca caaactcgtc gaagtgcatg
     1441 aagatttgcc ggagttacca cgcccgcaaa tcaccgacac ggaaaaacgc atcggtcaat
     1501 acgtggctca acttgtagac aacggcgcta cgctccagct tggtatcggt ggtattccgg
     1561 atgccgtatt ggcatcgctc gccgacaaac atgatttggg tattcatacg gagatggttt
     1621 ccgacggtgt gatacaggcc atcgaatccg gtatcgttac gggttccaaa aaaacactac
     1681 atccaggaaa agtgatcggt actttcgcac tcggctccaa acacctctac aattatatcg
     1741 ataataatcc gatgttcgaa tttcgtcctt cggattatac caacgatccg ttcatcattt
     1801 cgcgtaatga aaatatggtc gcggttaatt ccgccatcga agtggatatc accggacaga
     1861 tttgtgctga ctccatcggc actaaaattt tctccggatt cggcggtcag gtggatttta
     1921 ttcgcggcgc cgcttacgcc aaaggcggca aaccgatcat cgcgctgccg gctacggcac
     1981 aaggcgggaa aacatcccgc atcgcaccgg ctcttaaatc gggagcaggt gtagtcacga
     2041 cgcgggccga tgtacactat atcgttacgg aatttggtat agcttatctt catggtaaaa
     2101 atttacgcga acgtgccgaa gcgctgatcg ccatcgcgca cccggatttc cgggaagagc
     2161 tatcccgcgc ggcgcacgaa cgatttaaat ataattctaa gtaagtaagg atacggcgat
     2221 gaaagccaga cctaaaccat ttgtaatgcc cgaattttgt aaaggctgcg aacgttgcgt
     2281 cgaagcctgc gccaaaggct gcatcacacc cgggcacgaa gtgcatcccg tcagcgggat
     2341 gataccgatc gaattgcatc tcgaagaatg ctccggctgc gggctctgta tcagcgcctg
     2401 tcccgaacct tttgggttac gaccgctgga aaacgaacct tttgtcgaaa tggtggatcc
     2461 gttttctctc aacggtccgc gccccacttc ctcgcaacct gccgaagata tggcggcacg
     2521 cctgatccca ttgccaccga gcgaaccgct tgtcatcaag ggtacgtacg cttccgcgat
     2581 cggcgcgcta ctcgcaggat gccgtcattt ttacggttat ccgattacgc cgtctaccga
     2641 aggttcggaa ctcatggcta aattattacc cgagctcaac ggcgtttttg tgcaggccgt
     2701 gagcgaagtg gcggcggtca attatctgta cggctgtgca tcggccggtt taccgtgtat
     2761 gacgtttact tcgtcacccg gatttagcct catgctcgaa ggtatttctt acatgatcgg
     2821 cgcagaatta cccgctgtat ttgtcaatgt catgcgcggc ggcccgggac tgggcaatat
     2881 cggcccggaa caagcagaca tcaaactcgt gtgccgcgga ctcggtcacg gtaatacgca
     2941 tgcgatcgta ctggcaccgt cttctccgca ggagatgatg gatcttacga tgcaggcgtt
     3001 tcagcttgcc ttcaaatacc gcaatcccgt gatcgtcgtc ggtgacggct atatgggcca
     3061 gatgaccggt aaagtggaat tgccggatca catgatcaaa cccggcttgc cggcatgggc
     3121 cgtatggggc gatgaagcac accgcggcaa tctgatttcg tccattcaac tcagcgaaca
     3181 ggatctcgaa aaacataatc tgatgatcaa tcaaaagtat gcgcacatga cgcaagacga
     3241 gcagcaggcc gatcttttcc ggtgcgatga tgcggatgta ctcatcgtag ccgccaatac
     3301 acctgcacgc atggccaaag gcgccgtaca ggaattgcgt cgtcagggga tgcgcgtcgg
     3361 actcttcagg ccgatcacat tgtggccttt cccgatagac cggctcaagc cgcttttgta
     3421 tcacgtcaaa cagatcatct gcgtcgaagc cggcaacgga caactggagg acgaattgcg
     3481 tttagcgatg agcaaagccg aactgccgat gcctcgggtg gaacatgtcc gtcattttgg
     3541 cggcgtgctt ccgcaaacac acgagatcgt tgaaaaagtt ttatcgatgg aagaggtgat
     3601 cgcatgaaaa cgagcgtatt ttatgaaaat tttgagcggc acgcccacgg cgaaggactc
     3661 aaaggtcatt cgacccacta ctgcccggga tgcggccacg gcttggcaca taagttttta
     3721 gccgagacga tagacgaact gggcatacag gatcgtacca tcgcgatttc acccgtcgga
     3781 tgcagcgtat ttatgtatta ttatctggat gtaggtaata cccaagccgc acacggacgc
     3841 gctccgtcgg tagcgatcgc tcataagatg gccaatccca attcgatcgt catcagttat
     3901 cagggcgacg gcgatcttgc atccataggc ctggcagaaa tcatgcaggc cgcgcaactg
     3961 ggcattccca tcaccgtgat ttttgtgaat aatgcaatct acggtatgac cggcggccaa
     4021 atggcgccca ccacgctgat gggacaaaaa acatccacct caccgcacgg ccgcgatacg
     4081 atgatgggta tgccgctgcg tatgtcagaa atcctcgcgc agatggacgg gcctgtctat
     4141 atcgaacgtg tcgctttgta cgacagcaaa cagcgcgtgc atgccaaaaa agccatcaaa
     4201 aaagctttgc aacttcaggt cgaaggccgc ggatttgcgt tcgtcgagat tttatcggaa
     4261 tgccccacgc atctcaagat gacaccttcg gaagcggaag cctgggtaga aaaagaaatg
     4321 acggctgtat ttccgctggg cgtcaaaaaa gacgtgacgc gcgaaccgtg gtttatgctg
     4381 gaggaaccga attttgatgc agccaagatg atggaaaaga tcaacgcttc atcgcaaaag
     4441 gaaaaacgtt tttgtacggc gttcccgcac cacatcacac cgcacgatat cgcgctgaag
     4501 ctcgcagggt ccggcggtga cggcgcacaa acggcggcta tgctgatcac ccgcgccggt
     4561 atcaatgaag gttttgatgc aactcatatt ccaagctacg gaccggagtc gcgcggcggc
     4621 acgtcctacg cggatgtaca catcgcggaa gaggaagtgc tttcccctgc ctcaccgcat
     4681 cccaatgtcc tcatagcatt caatgcgcca agccttctca aatttggtcc gcaggtcgcc
     4741 tccaatggta ttattttata cgacagctcc gtgatcccca atccacctaa ctttaatcac
     4801 ggcgtacgta tgtatggcat tccttttact cagatcggta tggatcttgg ccgggcgatg
     4861 gtcaaaaata tcgtcgcgct cggcgcactc caggcggcga ctcagatgtt tcccaaagat
     4921 acattcctta ccgccattcg tcaggcgctc aaagacaaat gcgccatgat cccgctcaat
     4981 gaagaagctt ttgattgggg aatccgggca ttccacggcg aaatagccaa gcccggtgaa
     5041 cccgtggtgt aatacgccgg ttaatggtag gacgactccc cttcttacga aggggagttt
     5101 tttttactac cgtcacgcac aagccatcca caatatgaca taaagcacat tttgtacgtg
     5161 tcgtttattt tatatccgtc ttcttctgtc gttcgcctag ccaatctata gtgatacggc
     5221 tcatgccgca agctacgcct atttcgtaca ttgatcatga gagcattagc aatcatgcta
     5281 cggctcaaat cacgttgaga ggttttttat gctactcaga tccatgaaga taaccatcat
     5341 cgtagctctg ggattaggaa tgctcaaatc ctgctcggac gacaattcat acgaaagtat
     5401 agccggcgaa tgggagggaa ccgtatttac cggcgaaggc
//
LOCUS       DAOQNQ010000219         5398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_6144_length_5398_cov_4.144675, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000219 DAOQNQ010000000
VERSION     DAOQNQ010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6144_length_5398_cov_4.144675"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PK754_11965"
     CDS             complement(<1..737)
                     /locus_tag="PK754_11965"
                     /EC_number="5.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012926079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyproline epimerase"
                     /protein_id="HNB10266.1"
                     /translation="MSRHTFSCIDAHTCGNPVRVVTSGGPVLLGANMSERRQHFLREF
                     DWIRKALMFEPRGHDMMSGSIFYPPIDPANDVAILFIETSGCLPMCGHGTIGTVTVLI
                     EQRLITPRTPGILRLEVPAGIVEAHYTIQHNKVTSVRIVNIPSFLHSQNLTVECPDLG
                     EITLDVAYGGNFYAIVDPQKNFKGLETISAGDVLRWSPIVRQRINEKYTFTHPENSTI
                     CGCSHLQWTGVPTQNEAHARNAVFYGD"
     assembly_gap    747..846
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(908..1546)
                     /locus_tag="PK754_11970"
     CDS             complement(908..1546)
                     /locus_tag="PK754_11970"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNB10267.1"
                     /translation="MKVSLTDQAYDKIEEMLVTLKFKPGTIISEPEIIERIGMGRTPV
                     REALLRLGKEHMIKAIPRKGIYINEIHITDHIALMETRRALERIIAMRSANRATAEQK
                     KELTRLSQRIVDAAKKNDIETFIHADHAFDLIISEACRNPYASEACASLRTHCRRFWY
                     RFHHDQDLVTSAKLHAEVMSAIVAGNEERAGTASDKLMDYLDQMTRVAMNKG"
     gene            complement(1549..2994)
                     /locus_tag="PK754_11975"
     CDS             complement(1549..2994)
                     /locus_tag="PK754_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNB10268.1"
                     /translation="MTTDIKRLYINGSWIDGGDTFANINPSDTSDLIAHYAVATADHV
                     HDAVASAKQAFPAWSLSTPQQRFNILDAVGAEILARQDELGRLLSREEGKTLPEGIGE
                     VVRAAMIFKFFAGEALRAGGERMASVRPGVMVDMMREPLGVVGLITPWNFPIAIPAWK
                     IAPALAFGNCVVFKPAELVPGCAWALTEILHKAGIPAGVFNLLMGKGSVIGRTIVEHP
                     YVNAISFTGSVGVGRQVAAQCAARLAKVQLEMGGKNPLVILDDADLSVAVNAAVQGAY
                     YSTGQRCTASSRLIVTEGIHDTFVKALTDKLQTLRIDDALAQGTDIGPVVDLTQMNKD
                     LEYITIGAQENAHLAFGGVALERSKKGYYLQPALFTETTSAMRINREEIFGPVASVIR
                     AKNYDEALAMANDTPFGLTSGICTTSLKFAEHFKRHAQAGMVMINLPTAGVDYHVPFG
                     GRKGSSYGAREQGAYAREFYTTVKTAYTFAG"
     gene            complement(3092..3988)
                     /locus_tag="PK754_11980"
     CDS             complement(3092..3988)
                     /locus_tag="PK754_11980"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNB10269.1"
                     /translation="MHSTWQGIFPATTTKFKPDFSVDYSAIEKHTAWMLEAGIHGLVV
                     LGSLGENLTLTEDEKLAILKTACNVCAGKIPVIATVMETSTAKACAFAEKAARAGAQG
                     LMVLPGVPYPSDARETITHYRTIAKATDIPIMIYNNPVAYKVDITPEMFAEMADEPHF
                     VAIKESSDNIRRVTDIYNRVGNRYRIFCGVDDLAMEALTLGADGWLAGLVVAFPHETV
                     AIYNLVREGKMKQALEIYRWFMPLLHLDVNTKFVQNIKLTEQLVGVGSEIVRAPRLPL
                     IGEERARVEHIVREALVKRPKL"
     gene            complement(4117..5124)
                     /locus_tag="PK754_11985"
     CDS             complement(4117..5124)
                     /locus_tag="PK754_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10270.1"
                     /translation="MFSTIFIIIAGTVITIAGITAYNKIKGLILRKPKGFHLNLVEFF
                     LVPVVALLIVFEIWSRIVGTQEVHALEMSGNTLWALEHVSRGTGNWGERFSLRNVFGI
                     RVSDGTYLYKKSMEGNFQRIAQKENIAWCWDDETIVGVESSTGDVKYTIDPDRFESLP
                     AFRDGVAGFDMKADGIIRATNKSGQTINIDPLHFTEIAAPPDTIASEPITASSDSFKF
                     AMSDTTNAQLVNAAGQIISKDILTHGRILQVDPVQKIIITRHYENNDPQDFIISAYLF
                     NGTLRWQVRYDQLGVRRFFLKKSLLAHSLIADERFIFSAGNFVVALDIATGRLLWKTA
                     L"
     gene            complement(5135..>5398)
                     /locus_tag="PK754_11990"
     CDS             complement(5135..>5398)
                     /locus_tag="PK754_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10271.1"
                     /translation="GRARVQSRRAVGLAVTAWHASRGEFSRRPAIEHLRHIAQDAALP
                     TPVQKAAARLIKNVNDRLSADYTLHPVRDARIIITYFLQRLTP"
BASE COUNT         1306 a         1271 c         1344 g         1377 t
ORIGIN      
        1 ttatcaccgt agaatacggc attgcgggcg tgggcttcgt tttgtgtcgg aacgcccgtc
       61 cattgtaaat gggaacagcc gcaaatcgtg gaattttcgg gatgggtgaa agtatatttt
      121 tcgttgattc gctggcgaac gatcggactc catcgtaaaa catcaccggc agaaatcgtc
      181 tcaagacctt taaagttttt ttgcggatcc acgatcgcat aaaaattacc accgtatgca
      241 acatccagtg tgatttcgcc aagatcgggg cattcgacgg taagattttg cgaatggagg
      301 aacgagggga tattgacgat gcgaacactg gtcactttat tatgttggat tgtataatgt
      361 gcctcgacga taccggcggg aacttccaag cggaggatgc cgggtgtgcg cggcgtgatc
      421 aaacgttgct cgatgagcac agtgactgtg ccgatcgtgc catgaccaca catcggcagg
      481 cagccgctcg tttcgataaa taatatcgca acatcattgg ccggatcaat cggaggataa
      541 aaaatgctgc ctgacatcat atcgtgaccg cgcggttcga acataagcgc tttacgtatc
      601 cagtcgaatt cgcgaagaaa gtgctgacgg cgttcactca tattagcgcc taagagtacc
      661 ggaccgccgc tggtaacaac gcgtaccgga ttgccgcagg tatgtgcatc aatgcatgaa
      721 aatgtatgtc gtgacataat gacctannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnaatt ctaaattcta aattctaaat tctaaattct aaattctaaa ttctaaattc
      901 taaattctta tcctttattc atcgcaacgc gcgtcatctg atccagataa tccatcagtt
      961 tgtcggaagc ggtacccgcg cgctcttcat tacccgcgac gatagcactc ataacctcag
     1021 cgtgcagttt agcggaagtt accaaatcct gatcatgatg aaaacgatac cagaagcgtc
     1081 ggcaatgcgt gcgaagcgaa gcacaggctt cggaggcata cggattgcga caggcttcac
     1141 tgatgataag gtcaaacgca tggtcggcat gaatgaaagt ttcgatatca ttttttttcg
     1201 cggcatccac gatacgttgc gagaggcggg tcagttcttt tttctgttcc gccgtagcgc
     1261 gattggccga acgcatagca ataatgcgtt ccagtgctcg acgcgtttcc atcaacgcga
     1321 tgtgatcggt gatgtggatt tcgttgatat aaatgccttt gcgcggaatg gctttgatca
     1381 tgtgttcctt gcccaatcgc aataaagcct cgcggaccgg agtgcgcccc atgccgatcc
     1441 gttcgatgat ttcaggttcg gatatgatcg ttccgggttt gaattttaac gtgacgagca
     1501 tttcttcgat tttatcgtag gcctgatcgg tgagactgac tttcatagtt accccgcaaa
     1561 ggtatatgcc gtttttactg ttgtataaaa ttcgcgtgca tacgcgcctt gttcccgcgc
     1621 cccatagctg gagcctttgc gtcctccgaa gggtacatga taatccactc cggcggtggg
     1681 cagattgatc atcaccatgc cggcctgcgc gtgccgcttg aagtgttctg cgaatttaag
     1741 cgaagtcgtg cagatacccg acgtcagacc gaacggggta tcgttggcca tagctaaggc
     1801 ttcatcataa tttttagcgc gtatcactga tgcgacgggg ccgaaaattt cttcgcggtt
     1861 gattcgcatg gcagaggtgg tctctgtaaa cagtgcgggt tgcaggtaat aacctttttt
     1921 gctgcgttcg agtgcgacac cgccaaacgc aagatgcgca ttctcttgtg cgccgatagt
     1981 aatgtattcg aggtctttat tcatctgtgt cagatcaacg accgggccga tatccgtgcc
     2041 ttgcgccagt gcatcatcta tgcgcagggt ttgaagtttg tccgtcagag ctttcacaaa
     2101 tgtatcatga ataccttcgg ttacgataag gcggcttgac gccgtgcatc gctggcctgt
     2161 ggaataatag gcgccttgta cggcggcgtt gacggcgacg gacagatcgg catcatctaa
     2221 gattacgaga ggatttttcc ctcccatttc cagttgtact ttggccaaac gtgccgcaca
     2281 ctgtgccgca acttgtcgtc cgacaccgac ggagccggta aagctaatgg catttacata
     2341 gggatgctcg acgatcgtac gaccgatgac agatcctttg cccatgagta agttaaacac
     2401 accggccgga attccagctt tgtgtaaaat ttcggtcaaa gcccatgcac aaccggggac
     2461 taattcggcc ggtttaaaaa cgacgcagtt accgaatgcc aatgccgggg ctattttcca
     2521 cgccggtatt gcgatcggaa aattccatgg cgtgatgagt ccgacgacac cgagcggttc
     2581 gcgcatcata tctaccataa cccccggtcg tacggaggcc atgcgttcgc cgccagcacg
     2641 cagagcttct ccggcaaaaa atttgaatat catcgcggcg cgtaccactt cgccgattcc
     2701 ttcgggcaat gtttttcctt cttcccgcga cagcaaacgg cctagttcat cttgccgtgc
     2761 taaaatttca gcaccaacgg cgtcaagaat attaaaccgc tgttgcggag tggaaagcga
     2821 ccatgcgggg aatgcttgtt tggcggatgc gacggcatca tgtacatgat cggccgtggc
     2881 gacggcataa tgagcgatca ggtcggacgt atcggacgga ttgatattgg caaacgtatc
     2941 gccgccgtca atccatgaac cgttgatata caaacgtttg atatcggtgg tcatgtgtaa
     3001 tatttgttta gatgagaact acagaatgtg taaccaaaga agtgctgacc aatgtacatc
     3061 cctgatcgga tatagtaaat cagatttttt gtcagagttt tggtcgcttg actaaagctt
     3121 cccggacgat atgttcgacg cgcgcacgtt cttctccgat caaaggcaat cgtggtgcac
     3181 gaacgatctc cgaaccgaca ccgaccaact gttccgtcag cttgatgttt tgtacaaatt
     3241 tggtgttcac gtccaggtgg agtagcggca tgaaccatcg gtagatttcg agcgcttgtt
     3301 tcattttacc ttcgcgcacg agattgtaga ttgctaccgt ttcatgcggg aaagccacta
     3361 ccaacccggc caaccatccg tccgcgccga gagtgagcgc ttccattgcc agatcatcca
     3421 cgccgcaaaa aatccgataa cggtttccta cgcgattata aatatccgtc acgcgtcgaa
     3481 tattatcgga cgattctttg atcgctacaa aatggggttc gtccgccatt tccgcaaaca
     3541 tttccggtgt aatgtctact ttataagcca ccggattatt atagatcatg atcgggatgt
     3601 cggttgcctt ggcgatcgtg cggtaatgtg tgatcgtttc acgggcatcg gacggatacg
     3661 gaacgccggg caataccatg agtccctgtg caccggcgcg cgcggctttt tctgcaaaag
     3721 cacaagcctt ggccgtggaa gtttccatca cggttgcaat gaccgggatt tttccggcgc
     3781 aaacgttgca tgccgttttg agtatggcga gtttttcgtc ttcagtgagg gtgaggtttt
     3841 ctccaagcga acccagaaca acgaggccgt gaattccggc ttccagcatc catgccgtat
     3901 gcttttcgat cgccgaataa tctacgctga agtcgggttt aaatttagtt gtcgttgcag
     3961 gaaatattcc ttgccatgtg gaatgcatgt tttatctcca tttgtaatat acgtgtaata
     4021 tatcacagaa tatttgaaag acaataaatt tttaaaacgc gattataaaa taatcgatcc
     4081 gatctgcgaa catagtttct ttaaagaagg gtattcttat aacgccgttt tccataaaag
     4141 tctgccggtt gcaatatcca atgcgacgac aaagttaccg gctgaaaata taaaccgttc
     4201 atcggcgatc aagctgtgcg ccagtaagct ctttttgagg aaaaaacgcc gaacaccgag
     4261 ttgatcatag cgtacctgcc agcgcaatgt cccgttaaaa aggtacgcgg atatgataaa
     4321 atcttgcgga tcgttattct cataatgccg ggtgatgatg attttttgaa cgggatccac
     4381 ttgcaaaatc cgcccgtgcg ttaaaatgtc tttagaaata atttgaccgg ccgcgttgac
     4441 caattgcgca ttcgtcgtat cggacatagc aaacttgaaa gaatccgacg aagccgtaat
     4501 gggttcggat gctattgtat ccggcggtgc agcgatttcc gtaaaatgga gcgggtcaat
     4561 gtttatcgtc tgaccggatt tattagtggc acgtatgata ccatcggctt tcatatcaaa
     4621 accggccacc ccatcgcgaa acgccggtaa cgattcaaat cggtcgggat caatcgtgta
     4681 tttcacatca ccggtcgaag attcaacacc gacgatcgtt tcatcatccc agcaccaggc
     4741 gatattttct ttttgagcga tacgttgaaa attgccttcc atggattttt tataaaggta
     4801 cgtgccgtcg ctaacgcgga taccgaatac attgcgcaga gaaaaacgtt cgccccaatt
     4861 accggtgccg cgtgagacgt gttcgagtgc ccagagcgtg ttgccgctca tttccagagc
     4921 gtgcacttcc tgggtaccca cgatgcgcga ccatatctcg aaaacgatca gaagcgccac
     4981 gaccggcacg aggaaaaact caacgaggtt aagatggaag cctttgggtt ttctgagaat
     5041 caggcctttg attttgttat acgcggtgat accggcaatg gtgataacgg taccggcgat
     5101 gatgatgaat atggttgaaa acataggcga tggattaagg ggttagacgt tgtaaaaaat
     5161 aagtgatgat aatgcgtgcg tcgcgtaccg ggtgcaacgt ataatcagcg gataagcgat
     5221 cgttgacgtt tttgatcaga cgtgcggcgg ctttttgtac gggcgtcggc agcgcggcgt
     5281 cctgcgcgat atgccgcaaa tgttcgatcg caggacgtct tgagaattcg ccgcgggagg
     5341 cgtgccatgc cgtgacagcc aatcctacgg cccggcgtga ttgtacccgc gcgcgtcc
//
LOCUS       DAOQNQ010000220         5360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_6212_length_5360_cov_4.449010, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000220 DAOQNQ010000000
VERSION     DAOQNQ010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6212_length_5360_cov_4.449010"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="PK754_11995"
     CDS             complement(<1..786)
                     /locus_tag="PK754_11995"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNB10272.1"
                     /translation="MHALDAIFKPRNVAVIGASRKSKTIGHEIIHNLIECGFTGAIYP
                     VNPNAPAIHSIKAYPTVKDIPDEVDLAIITVPKQLVLGVVDECAAKGIKGLIVITAGF
                     KEVGAEGAALEDQLMAKIKKNDMRMVGPNCMGVINTDEHVNLNATFSPTQSRSGNIAF
                     ISQSGALGVAILENAESLELGISNFASIGNKPDISGNDLLEYWRDDSRTKLILMYLES
                     FGNPRRFIEVARSITRFKPILAVKSGRTMAGARAASSHTGALSG"
     gene            970..2151
                     /locus_tag="PK754_12000"
     CDS             970..2151
                     /locus_tag="PK754_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNB10273.1"
                     /translation="MPKYPHTAERVKELTGSVYEKYRHLIKQQTSVAKLHIGDTALWP
                     PYAMPLADYFTSAHRDMTKYCNTYGVDELRKSIAEKVSEDNGITSTRDHVLVTAGASN
                     ALSAAMMTLINPGDEVIVLTPCWPFFTGMVRAAGGKVVEVPLYMDLYDQPEMDIFGKL
                     KNATTSKTVAVYVNSPNNPSGKMLDARQWQTVVDFVKQHQLWLISDEAYDGLAFDGRK
                     TISPATLGIREQTISIFTFSKIFMFAGLRLGYAIAEPELIHALNKVMVHQLYSPSPVG
                     QYMMVEPLRRRHEWMHGINAQYQQLRDAMVEALAMPVAKPEGTYFVFLDAAPYLQGRA
                     FDQLVEQMIMGGVLVAPGGDFGKDFSSYIRLCFTGEPESVITSSAQVIRNVLLQNGQT
                     S"
     gene            2174..2959
                     /locus_tag="PK754_12005"
     CDS             2174..2959
                     /locus_tag="PK754_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNB10274.1"
                     /translation="MKIGYVQFKPQFGDIDYNAAKIYALLEKKQADLMVLPELATTGY
                     YFRDRKEAYALSEDPKKSFVVEVLVELCKKNGMHVITGFNERNGRKNYNSALLIGKKG
                     IVDVYRKIHLFNTEKNCFTPGDKPLRVHKIASARVGMMICFDWIFPEVTRTLSLMGAD
                     IIAHPSNLVLNYCQNAMITRSIENMVYCVTANRYGEETRPHGDLTFTGQSQIVAPKGV
                     LLHRAPEQKEEVYITKIDLTKSRQKNMTPKNDLFKDRRTKFYL"
     gene            2996..4918
                     /locus_tag="PK754_12010"
     CDS             2996..4918
                     /locus_tag="PK754_12010"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family peptidase"
                     /protein_id="HNB10275.1"
                     /translation="MNYRTLLWWVCISASVSAQQVNYEVRFPNIRHHEAEVTAVFSDI
                     SSPVLDIRMSRSSPGRYALHEFIKNVYSVRAEDGRGKKLDVTQNDPYGWQVSAHDGTV
                     RFFYTVYGDHIDGTYLSLDASHAHINPPAMFVYAQNADHRNIRLRFTLPDKTWRIATQ
                     LKESGASVYIASDLAYLMDSPIEISAFQESSWRVPATSGDILFSLILHHTGTPKQTEY
                     LAKVGDGIVREARAVFGEFPVFDQGRYAFMMELTPYASWDGMEHRNSCVITNRDSING
                     DFLDIASTMAHEFFHAWNVERLRPKSLEPFDKARVNMSEELWFAEGFTSYYDDLIITR
                     AGIQSVDRYCKVLTKLMNNVVVDPSKNFGGPAHMSQRAAFTDAATSVDAQNRKNIFST
                     YYWYGATVALGLDLTLRAEHGKTLDDYMQLLWKAYGKTEKPYTSADLESALGDLIGDK
                     KRAQRFFDETIRGNRLPDYKKLLDYAGFVTRAAHADRPTLGQVRLAADDDGILIKDAT
                     LIGDPLYLAGLDRGDKIFSLNDQAVHSIAAWEEVLTQLKPDDSVTIVYEQRGLKGISR
                     VKVAADPSLEIIPVEQTGKPLSEKKSNFRAAWLSSKSTDKPLLYRYCKKCRRAYEFQY
                     EFCPFDGVLLQITPEE"
     gene            4922..>5360
                     /locus_tag="PK754_12015"
     CDS             4922..>5360
                     /locus_tag="PK754_12015"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNB10276.1"
                     /translation="MHLETMKAAVLQVVGSIENLHYSDISKPEPKPGEALIRINAAAL
                     NHRDLWIVFGKYAKIQVPAVLGSDACGTVEQVGDMKDNIWVGRRVVVHPGLFWGNNPK
                     AQGKNYKILGMPDQGTLAEYVTVPVENLAEAPGHLSDEEAAALP"
BASE COUNT         1404 a         1166 c         1370 g         1420 t
ORIGIN      
        1 accggacaag gcgccggtat gagacgatgc ggcacgcgct cccgccatgg tacgtcccga
       61 tttgacggcc aatatcggtt tgaatcgcgt aatactgcgc gctacttcga taaagcgccg
      121 gggattgcca aaactttcaa ggtacatcaa tatcagttta gtgcgcgaat catcacgcca
      181 gtattccaaa agatcattgc cggagatatc cggtttatta ccgatgctgg caaaattgga
      241 aatgcccagt tcaagcgatt cggcgttttc gagaatggct acgccgagcg cgccgctttg
      301 ggagatgaat gcgatattgc cggagcgcga ctgggtcggc gaaaacgtag cattgagatt
      361 gacgtgttca tcggtattga tcacgcccat acagttaggg ccgaccatac gcatatcgtt
      421 tttttttatt ttggccatga gttgatcttc gagtgccgca ccttccgcac cgacttcttt
      481 gaatcccgcc gtgatgacga tcagaccttt gattcctttg gccgcgcatt cgtccacgac
      541 gccgagtacc agttgtttgg gcacggtgat gatggctaga tccacttcgt ccggaatatc
      601 tttgacggtc ggataagctt tgatactatg tatggccggt gcattcggat tgacgggata
      661 tatcgcgccg gtgaaaccgc attcgatgag gttatgaatg atttcatggc cgatggtttt
      721 gcttttgcgt gaagcgccga tcacggcgac gttgcggggt ttgaatatag cgtcaagagc
      781 gtgcacgttt ttttcctttg cgggttgtag ttttatcagt aatttgaacc gcaaaaaata
      841 tatgcattcc ggcggcaaaa tacatgagca ttgtcatgtt tacttttatg cgataaaatt
      901 ttcaataaag ttaaagttcc aaagcatcaa acataacgac attatatatt aattaatttt
      961 attcccatca tgccaaaata tcctcataca gccgaacgcg tgaaagagct taccggatcg
     1021 gtgtacgaaa agtaccgtca tttgatcaaa cagcagacat cggttgctaa attgcatatc
     1081 ggagatacgg cgttatggcc gccgtatgcg atgcccttgg ccgactattt cacatcggca
     1141 caccgtgata tgaccaaata ttgcaacacg tacggcgtgg atgagctgcg taaaagcatt
     1201 gccgaaaaag taagcgaaga caacggtatc acttccactc gggatcatgt tttagtgacg
     1261 gccggagcga gcaatgcatt gagtgcggcg atgatgactc tgatcaatcc cggcgacgaa
     1321 gtgatcgtgt taacgccgtg ctggccattc tttacgggaa tggtgcgtgc cgcgggcgga
     1381 aaagtggtcg aagttccgct gtacatggac ttatatgacc aaccggaaat ggatattttc
     1441 ggaaagctca aaaatgcaac gacttccaaa acggtggccg tgtatgtcaa ttcgcccaat
     1501 aatccgtcag gaaaaatgtt ggatgctcga caatggcaga ctgtcgttga ttttgtaaaa
     1561 cagcatcagt tgtggctgat cagcgatgaa gcatacgacg gtttggcatt tgacggacgt
     1621 aaaacgattt cccccgctac gttgggtata cgcgaacaaa ccatcagcat ttttacgttt
     1681 tctaaaatat ttatgttcgc gggattgcgc ctcggctacg cgatcgcgga gcctgaactt
     1741 atccatgcat tgaataaagt catggtgcac cagttatata gcccttcgcc ggtggggcag
     1801 tatatgatgg tcgaaccgtt gcggcgtcgg cacgagtgga tgcacgggat caatgctcaa
     1861 taccaacaac tgcgcgatgc gatggtggaa gcactggcca tgccggtcgc taaaccggag
     1921 ggaacgtatt ttgttttttt agatgcagcg ccgtatttgc aaggtcgggc atttgatcaa
     1981 ctggttgagc aaatgattat gggcggcgta ttggtcgcgc cgggcggtga ttttggcaaa
     2041 gatttttctt cgtatatacg cctctgcttt acgggtgaac cggaatctgt aatcacatcg
     2101 tcggctcagg ttattcgaaa tgtattattg caaaacggac aaacgtcata aaaatctgta
     2161 taggaatgaa aacatgaaaa tcggatacgt gcaattcaaa ccgcaatttg gcgatataga
     2221 ttataatgct gccaaaatct acgcactttt agaaaagaag caggctgatc tgatggtatt
     2281 acctgaattg gccacaacgg gatattattt tcgcgatcgt aaggaagcgt atgcgttatc
     2341 cgaagatccg aaaaaatcat ttgtcgtgga agtactggtc gagttatgca aaaaaaacgg
     2401 tatgcacgtg atcaccgggt tcaatgaacg gaacggacga aaaaattata acagcgcctt
     2461 gctaatcggt aaaaaaggca ttgtggatgt gtaccgtaaa attcatttat ttaacactga
     2521 aaaaaattgt ttcacaccgg gcgataaacc gctgcgcgtt cataaaatcg cttcggcacg
     2581 ggtcggtatg atgatttgtt tcgactggat ttttcccgaa gtgacgcgca cactgtcact
     2641 gatgggcgcg gacatcatcg ctcatccttc caatctggtt ttgaactatt gccaaaatgc
     2701 catgattacc cgatccatcg aaaatatggt gtattgtgtc acagccaatc gttatggcga
     2761 ggaaacacgt ccccacggcg accttacctt tacgggacaa agtcagatcg ttgcccccaa
     2821 aggcgtgtta ctacatcgtg cgccggaaca aaaggaagaa gtctatatca caaagattga
     2881 tttgaccaag tcacgtcaaa aaaacatgac gccgaaaaac gatcttttca aagatcgaag
     2941 aacaaaattt tatctctgag gcctttttca ttttctcatc ttagtagagg ttaatatgaa
     3001 ttatcgtacc ctgttatggt gggtttgtat atccgctagt gtatctgcac aacaagttaa
     3061 ctatgaagtt cgctttccca acattcgcca tcatgaagcg gaagtgacgg ccgtgttttc
     3121 ggatatttcg tcacccgttt tggatattcg gatgagccgt tcctcaccgg ggcgttatgc
     3181 actgcacgaa tttatcaaaa atgtatattc ggttagagct gaggacgggc gcggaaaaaa
     3241 acttgatgta acccaaaatg atccttacgg gtggcaggta tccgctcatg acggaacggt
     3301 gcgttttttt tatacagtgt acggcgatca tatagatggc acgtatttat cgctcgatgc
     3361 gtcgcacgcg catatcaatc ccccggcgat gtttgtgtat gcacaaaacg cagatcatcg
     3421 caatatccgc cttcggttta cgcttccgga taaaacatgg cgcatcgcta cacaactcaa
     3481 agagtccggt gcttcagtat acattgcttc ggatttggct tatctgatgg atagtcctat
     3541 tgagatcagc gcatttcagg aatcgtcatg gcgcgttccg gcgacaagtg gtgatattct
     3601 tttttcgctg atacttcatc atacgggaac gccgaaacag acagaatatt tggcaaaagt
     3661 cggcgatggg atcgtccgcg aagcgcgtgc ggtgtttggt gaatttccgg tttttgatca
     3721 gggacggtat gcctttatga tggaattgac gccgtatgcg tcctgggacg ggatggaaca
     3781 ccgcaattca tgcgtgatca ccaatcggga ttctatcaac ggtgattttt tggatatcgc
     3841 atctacgatg gcgcatgaat tttttcacgc ttggaatgtg gagcgtcttc gtcccaaatc
     3901 gctagaaccg tttgataaag cgcgggtcaa tatgtcggag gaactttggt ttgccgaggg
     3961 ttttacttcc tattatgatg atttgatcat tacacgggcg ggcattcaaa gcgtggatcg
     4021 gtattgcaag gtattaacca aattgatgaa taacgttgtc gtggatccct ccaaaaattt
     4081 tggcggtccg gcacacatga gccaacgtgc ggcgtttacc gatgccgcga cttcggtgga
     4141 tgcgcaaaat cgtaaaaaca tattttcgac gtattactgg tacggcgcta cggtggcttt
     4201 gggattggat ctgacgttgc gcgcggaaca tggcaaaacg ttggacgact acatgcagct
     4261 tttgtggaaa gcatacggta aaacggaaaa accctatact tcagccgatc tcgaatcggc
     4321 gttaggtgat ctgatcggcg ataaaaaacg ggcgcaacgt ttttttgatg aaacgattcg
     4381 cggtaaccgg ttaccggatt ataaaaagct gctggattat gccggtttcg tcacacgtgc
     4441 tgcacatgcg gatagaccga cattaggaca agtgcggctt gcagccgatg acgacggtat
     4501 cctcatcaaa gatgccacgc tcataggcga tccgctgtat ctggccggtc tggatcgcgg
     4561 cgataaaatt ttttcgctca atgatcaagc tgtgcattcc atcgcggcgt gggaagaggt
     4621 tttgacacaa ttaaaacccg atgacagcgt aacgattgtt tatgaacagc gcggcctcaa
     4681 aggtatatcg cgggtcaaag ttgctgcaga tccgtctttg gaaattatac cggtcgagca
     4741 aaccggtaag ccgctgtcag aaaaaaaatc aaactttcgt gcagcatggc tttcatccaa
     4801 atctactgac aagccgttgt tgtatcggta ctgcaaaaag tgtcggcgtg cgtacgaatt
     4861 tcaatacgaa ttctgcccgt ttgacggcgt gcttttacaa ataacaccgg aggagtaaat
     4921 cgtgcatctg gaaaccatga aagcggcggt attgcaggtg gtcggctcta ttgaaaattt
     4981 gcattattcc gacatctcta aaccggaacc taagccgggt gaagcgttga tccgaattaa
     5041 tgcagcggct ctgaatcatc gtgatctctg gatcgtattt ggtaaatatg ccaaaataca
     5101 agttccggct gttttgggtt ccgatgcatg cggcacggtt gagcaagtcg gtgacatgaa
     5161 ggataatatt tgggttggtc gccgtgttgt tgttcatccc ggtttgtttt gggggaacaa
     5221 cccgaaggca cagggaaaaa attacaaaat cttgggcatg cccgatcagg gtacgcttgc
     5281 ggagtatgtc acggtaccgg tagaaaattt agccgaagcg ccggggcatc tttcggatga
     5341 agaagcggcg gcgttgccgt
//
LOCUS       DAOQNQ010000221         5357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_6220_length_5357_cov_4.917390, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000221 DAOQNQ010000000
VERSION     DAOQNQ010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6220_length_5357_cov_4.917390"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1728)
                     /locus_tag="PK754_12020"
     CDS             complement(<1..1728)
                     /locus_tag="PK754_12020"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10277.1"
                     /translation="MKISVICLLFLTCASKIGFTQEYMKLEPLFKLKDGWEINGYSPD
                     GKYVVVNKSNDYKLININNGEKIVDLSWKYVFSADSRFIAHGTEKINIFDITNSAEYQ
                     SPPVENSHQENIAIDPDRKFLAYHVVTNDYEDFRIGIWDFVNGKMKKEINLKATKLDG
                     KVLIYTRSYFFFTPDGRYLFAFLKRTDYGGGVDRGWSLVVFSTDNWKTYATLDWGNVI
                     EDNPKPIFFSEDGSIMVFSTQLSIHFYSFAENKFFNSIRVANFKETSGKYFRSNNYYA
                     ISITSKALAVLNPSNPSNVDVHALLSGSKLYTILCNFDTSGGGANYFINPVFSFSFSR
                     DGNLLAIQGWKFNSDSTKALNDRNRYAYMNSPYRGNYEYKDGRYYNRLQSIYKVADGK
                     LITHIRSTEIDDESIGSQPEWFDDSAILNGQYICNKGELYSIKNGKNLQQVDWRWYTF
                     PNDRVAVESNYKHNGHYLQLYRASYSDHHELSLMLDKPNFQQSFSMMSGAEVKEKELK
                     KALKNPEVTDLDLHGQNISKIGKEIGNLTNLRHLYLNLNRLKRLPDEVGNLRELQYLN
                     LSYNSLSELP"
     gene            complement(1881..2276)
                     /locus_tag="PK754_12025"
     CDS             complement(1881..2276)
                     /locus_tag="PK754_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10278.1"
                     /translation="MKSLMRSLNYAVIGLAAISAFANDVFSQASYDYVSEYSGVEFYS
                     RWKKDPYTNDSHLNVKVINTSQETKRISFAIQWLDREGRFIEKSTNTGFTIRPGQTKA
                     GDLDGLYWYVPKGYSSHNVEAKLVDIEIR"
     gene            complement(2491..3657)
                     /locus_tag="PK754_12030"
     CDS             complement(2491..3657)
                     /locus_tag="PK754_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10279.1"
                     /translation="MRRSQKQFYFIALLTLYGYQVPLLSQVAILGYTTKFNFDGGFEY
                     NTWSPHKTNELKYDIEGFRIGYVRGELVHDIPFIPDMKFNWETNFGAKHQEEILKAHN
                     SMSKLEQGYEKMNAILSFFPESKIKKEGDEKVEMKNRDGSVRKYHNFEISYTKETFYI
                     GVTPATEGLNYAAYASGKTYGFPYGTSISQFTKFEEISATFYTDGKLYIFWIINAFFS
                     AAADRESAFSDESRIFQFPEADSRIGVFYSTFQKPYSVNQVLTQGEVINSPNTIYNAR
                     FQAAGIIDEISSPAQSSLIARWTNRFGYSWINLRKKEDLEDTNSPGFFYFGTEAELGF
                     RIHFNKFSLNLSASGNFGFMQGLNVDTEAEQFYATSFINNDLLLKYKGSLQYIF"
     gene            complement(4279..>5357)
                     /locus_tag="PK754_12035"
     CDS             complement(4279..>5357)
                     /locus_tag="PK754_12035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNB10280.1"
                     /translation="KTTPVNSSFPITVTSERWTCIGNPYDFNVAWDSVGDRDHVNTPI
                     WYDGTENDWKVANVLEPWKGYFVFTTERTRDIKIPAKESGLAKRIEQNTFKKYQSGEW
                     QIQIKTCGEGLNDQYNFVGMFSRASDHYDLEDRVESPRHPGNEFIKLYFDHSDWPEHA
                     FRYGSDFRPIRSDGDVWDFDVNALTSAEKIPLNFEQLENVPPDFEIILLDKDLKITRD
                     LRKEPGYVFPAQRNVQFTRHFRIVIGKKDFVSRNLNGVSVTPEKFDLSQNYPNPFNPT
                     TGIKFSLASYSRVRLTIYNMLGQEVKTLVNENLQAGYQTVLWDGRNAAGQAVSSGVYL
                     YRLQAEALDTKQRYTKTRKMLLVK"
BASE COUNT         1529 a         1137 c          983 g         1708 t
ORIGIN      
        1 aggcaattcc gacaaagagt tataggatag attcaaatat tgcagttccc tgaggttccc
       61 tacttcatct ggaagccgct ttagtctgtt gagattcaaa tagagatggc gcaaatttgt
      121 taagtttccg atttcctttc caatcttact gatattttga ccatgtaggt caagatcagt
      181 gacctcggga ttttttagtg cttttttcaa ttccttctct ttgacttccg cgcccgacat
      241 catcgaaaat gactgctgga agttaggctt atcaagcatc aacgacaatt cgtggtgatc
      301 ggaataagaa gcccgataca actgtaagta gtggccgtta tgtttatagt tactttcaac
      361 agcaacgcga tcgttgggga atgtatacca tcgccaatca acctgttgta aattctttcc
      421 attcttaatg gagtataatt ctcccttgtt gcaaatatat tgaccattca aaatagctga
      481 atcatcaaac cattctggtt gagagccaat cgattcgtca tcgatctcag ttgatcttat
      541 gtgcgtgatc agtttgccat cggcaacctt atatattgat tgcagtcggt tataataccg
      601 tccatcttta tattcataat tcccacgata aggagaattc atgtatgcat atctattcct
      661 atcatttaac gctttcgttg agtcggagtt aaatttccag ccttgtattg ccaaaagatt
      721 cccgtctcga gaaaaactaa aactgaatac cggattgatg aagtaattag cgcctcctcc
      781 acttgtgtca aaattgcaca gaatagtgta taattttgag ccagacagta aagcgtggac
      841 atctacattg gacggattgc ttggatttag aacggccaat gcttttgacg ttatagatat
      901 ggcataataa ttattcgatc gaaaatattt gcctgatgtt tccttaaaat tagcgactct
      961 gatactgtta aaaaacttat tttctgcaaa cgagtaaaaa tgtatgctta attgggttga
     1021 gaacaccatt atgctaccat cttcagagaa aaaaataggt ttcggattat cctcaattac
     1081 attcccccag tcgagagtag catatgtttt ccaattgtcg gtcgaaaaga caaccaatga
     1141 ccatcctctg tcaacaccac cgccatagtc cgttcttttc aagaacgcaa ataggtatct
     1201 tccgtccggc gtaaagaaaa aatacgaacg agtgtagata agcactttac catccaattt
     1261 ggtcgcttta agattaattt cctttttcat tttaccgttc acaaaatccc agattccaat
     1321 acgaaagtcc tcataatcat tggttactac gtggtaagct aaaaatttgc gatccggatc
     1381 gattgctata ttttcctggt gagaattctc gacgggcggg ctttgatatt ctgcactatt
     1441 tgttatgtca aaaatattaa ttttctccgt tccatgagca atgaatcggg aatcagcgct
     1501 aaaaacatac ttccaagaca aatcaacgat cttttctcca ttgttaatat ttatcagttt
     1561 gtaatcattg gatttgttaa ctactacata cttaccatct ggagaatacc cattgatctc
     1621 ccatccgtct ttcagtttga aaagcggttc aagtttcatg tattcctgcg taaagccaat
     1681 tttggaagcg caagttaaaa aaagaaggca aattacactg attttcatat tgtactccga
     1741 gttaaattga tcgtgtgaat atcaattatg tatagcgagt ttccattctc tttcatgttt
     1801 ccgcttcgca aatttctgat agaatgaagt tcaaggcgta agcccgttac tgtaggagaa
     1861 gcggacatac aactgacttc tcacctgatt tcaatatcca ccaatttggc ctccacgtta
     1921 tgggaagagt accctttggg aacgtaccaa tacagcccat ccaaatcacc agcctttgtc
     1981 tgtcctggtc gtatagtgaa accggtatta gttgattttt ctataaaccg tccctcccgg
     2041 tcaagccact gtatagcaaa agaaatgcgt ttggtttcct gagatgtatt gatgactttt
     2101 acgttgagat gcgaatcatt agtgtacgga tccttcttcc atcgcgaata gaattcaact
     2161 ccgctgtatt cagaaacata gtcatagctg gcctgtgaaa aaacatcgtt ggcaaatgcg
     2221 gaaatagccg ccaacccgat gacagcataa tttaagctac gcataaggct tttcatgatt
     2281 cgtacctttc atgtgtgtta atagtggatg ttcgcgcacg tgattaccat tgatttaaag
     2341 gattcttatt gaaccgttat ggaacctcca gattatatcg aataagcttc gaggcatctt
     2401 ctaataaccc gcactgccgc attctaagca acgggttaat tgggtaccat tacatgatgt
     2461 accaacgccg actactcaat atgacctctt ctagaaaata tattgtagcg atcctttgta
     2521 tttcaggagt agatcgttgt tgatgaaact tgttgcgtaa aattgttccg cctcggtatc
     2581 tacgttgaga ccttgcataa acccaaaatt gccgctggca gagagattta agctaaactt
     2641 gttgaaatgt atcctgaatc ccaattcagc ttcggtacca aaataaaaaa aaccaggact
     2701 gttggtgtct tcgagatcct ctttttttct gaggttgatc cagctgtatc caaaacgatt
     2761 ggtccatcgc gcaattaacg atgactgcgc aggggatgat atctcatcga taataccggc
     2821 ggcttgaaat cgtgcattat agatggtatt aggagaattg atcacctcgc cctgcgtcaa
     2881 cacttgattt acggagtagg gtttttgaaa cgttgaataa aatacaccga tccgactgtc
     2941 agcttcgggg aactgaaata ttctcgattc atcactaaat gcggattcac ggtctgccgc
     3001 cgcggaaaaa aaagcattga ttatccagaa aatatacagt ttaccgtctg tataaaaagt
     3061 ggcgctgatt tcttcaaatt tagtaaattg tgaaatactt gttccgtatg ggaaaccgta
     3121 cgttttgcct gaagcgtatg ccgcataatt aagcccttcg gtagcggggg taactccgat
     3181 gtagaacgtt tcttttgtat aagatatctc aaaattgtga tactttctaa cgcttccatc
     3241 acgatttttc atttcaactt tttcatcccc ttcttttttt atttttgatt ccggaaaaaa
     3301 agagagaata gcattcatct tttcgtagcc ttgctctagc ttactcatag agttgtgagc
     3361 ttttaatatt tcttcctgat gttttgctcc aaaattagtt tcccaattaa atttcatatc
     3421 tggaatgaaa ggaatatcgt gtaccaactc gcctcgaaca tagccaatcc gaaagccttc
     3481 tatgtcgtac ttcaactcat ttgttttgtg cgggctccat gtgttgtatt caaatccgcc
     3541 atcaaagtta aatttagttg tatagccaag tatagcgact tgacttaata gcggaacttg
     3601 atatccatat agagtcagca acgcgatgaa gtaaaattgt ttctgtgatc tacgcataag
     3661 ctttcctcct tctatttttc attctaaaca cttggaatta ctttgaatat ctgcagatag
     3721 ttttttttaa aacagtctat ccataagaaa catatagcat ctgaacataa ctttacgcaa
     3781 aactgattgg atttaatgtg tcaatctcat tccttgtaca ttcatcctat gaagttaccg
     3841 ctcacagcaa aacgaggaag aagtatgcaa aaaaataacc ctccccgaac cttactggtt
     3901 taagttcaat tttttggttt ctgattattc acacggcaaa cccgctcgtg tttttcgata
     3961 tacttttagt aacatgattt tgtcctccta aaacatttat cagttatcca gttatcagtc
     4021 ttcagtgatt tatgattaag tggaatcaat gtgataaaaa tatgtttttg cctgtttctc
     4081 ttttagacgc ttttggaatt aaaatcataa ccaaacatta aacaactaca cgccttctct
     4141 ccgtgttttt ttctgaaatc gttagtatta aaccgatata tacgagtcat ctattatcgg
     4201 catgccgtac ggaattattg tgtttgccat aacctgtgga tcaacatgat cgcacgacgt
     4261 aaaccggtaa tagtcatctt acttcaccaa tagcatcttg cgggttttgg tatatctctg
     4321 tttggtatcc agcgcctccg cctgcaggcg atacaaatac acgccgctcg atacagcctg
     4381 ccccgcagca ttacggccat cccacaacac ggtttggtat cccgcctgta aattttcgtt
     4441 taccagcgtt ttcacttcct gacccaacat gttgtatatc gtcaatctta ctctggaata
     4501 cgacgccaat gaaaacttga tcccggtagt cggattaaag ggattgggat agttttgact
     4561 tagatcaaat ttttcgggag tgaccgatac gccattcaga ttgcgcgaca caaaatcttt
     4621 ttttccaatt acaattctga aatgcctagt aaattgaaca ttcctctgtg cagggaaaac
     4681 gtagcccggt tccttcctaa ggtcgcgcgt gattttgagg tctttgtcga gtaaaatgat
     4741 ttcaaaatct ggcggcacat tttctaattg ttcgaaattg agcggaatct tctcagccga
     4801 ggttaaagca ttgacatcaa aatcccacac atcgccgtcg gagcgaattg ggcgaaaatc
     4861 agaaccataa cgaaatgcat gctccggcca atccgaatgg tcaaaataga gtttgatgaa
     4921 ctcatttccc ggatgacgcg gggactcgac ccgatcttct aaatcatagt gatcggatgc
     4981 ccttgaaaac attccgacaa agttgtattg gtcattaagc ccttcgccac aagtcttaat
     5041 ttgaatctgc cattcgccgg attgatattt tttgaaagta ttttgttcaa ttctttttgc
     5101 taacccgctc tcttttgccg gaatcttgat atcacgtgtt ctttcagtag tgaaaacaaa
     5161 ataacctttc caaggttcaa gaacatttgc aaccttccag tcattttctg ttccatcata
     5221 ccaaattggc gtattaacat ggtctctatc tccgacactg tcccatgcca cattgaaatc
     5281 ataaggattt ccaatgcacg tccatctctc actagtcaca gtaattggaa aacttgaatt
     5341 aactggcgtc gttttta
//
LOCUS       DAOQNQ010000222         5345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_6244_length_5345_cov_8.396408, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000222 DAOQNQ010000000
VERSION     DAOQNQ010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6244_length_5345_cov_8.396408"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="PK754_12040"
     CDS             complement(<1..1182)
                     /locus_tag="PK754_12040"
                     /inference="COORDINATES: protein motif:HMM:NF016732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HNB10281.1"
                     /translation="MQDKQGFFRDLYESFKAGAYYLKSKRAADRPFYNTLGLSGQIVT
                     ANFNDTARVDVQQNLKTNTEWVALLCDVNAYWVAQATDNYSIYNIINRDERKELDNHY
                     AFSLLEDGNPDVTFFDMMYLMEYANQLIDGLPFYITPLTMRGEGGGIIPKSFTPLYPD
                     MGMLITKRDDYGRTTGYEWQGGGKVMDIPEDQILWYRLPNIGNSLGGGGWVNKALRLA
                     NLDKQQKQHNLETVMSKGVPPGYVKFNEAMLETKFQESMAKFSRSFKSAARKGNLFGT
                     DTNAEYTPLGFSAVELDMINGMNLTRDQLHQMAGVPRVMTGQPDANTMAEAKIQFALY
                     KMNRVNKSLKSYNQFFTKMLNRYFPQKNGSKLSFEFDLSVPEDDLMKQQIREYKLRTG
                     QA"
     gene            complement(1367..2566)
                     /locus_tag="PK754_12045"
     CDS             complement(1367..2566)
                     /locus_tag="PK754_12045"
                     /inference="COORDINATES: protein motif:HMM:NF016359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HNB10282.1"
                     /translation="MNEVRFETTPIFDWTYNSAKPIVIHQGGTGSGKTYGIDQYLLIR
                     AIEARNTITIVGQDVPNLRGGALRDMQTIIKGNEWLKNYIVDYNATNREYHFTNGSII
                     EFKSFDDPQDAHSGKRDYSFFNEANGIPFSVFDEIYVRTTKQTIIDYNPTNEFWAHEM
                     IRDMQAGKRNDVDLFISNFTHNRFLPPAIVEKILSYKVTNPNRWLVYGLGKTGTIEGL
                     IFPDLQVVDAFPKDAERVAYGLDFGFTNDPTVLIRSTVLDGMIIADQMIYETRLDNPA
                     IARRLKELGLAGELIYCDSAEPKSIKELRDLGCNVVEAVKGAGSINWGLSHLKSYRGG
                     YAVTARSSEMRHELRNYSNKYDKFTNRYTNDPEDKFNHCADAWRYSQSGILHPTSANF
                     GTAEDDE"
     gene            complement(2559..2978)
                     /locus_tag="PK754_12050"
     CDS             complement(2559..2978)
                     /locus_tag="PK754_12050"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB10283.1"
                     /translation="MKNQKIYWIMATKFNIQKRAMLEALEKSLGVVTTAAKVVGIDRT
                     THYKWMTSDKKYKKAVENIDNVALDFVESKLFKSIEKGRDAPIIFYLKTKGKKRGYIE
                     RQEITGLDGEILGGSPTIIVNASNVSPIKDEKEIKDE"
     gene            complement(2975..3328)
                     /locus_tag="PK754_12055"
     CDS             complement(2975..3328)
                     /locus_tag="PK754_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10284.1"
                     /translation="MNEARKQRGPMISCPKCGHQIKVGRSKGQRGYYFEVIVKAYSEA
                     MGEEDLDLVNNWLKIKFNPKFDNVGGDEIKYGGSIEKEPVHRIEELYEQMRAFAMNFY
                     GVYIPEPNEVPLAEL"
     gene            complement(3321..3590)
                     /locus_tag="PK754_12060"
     CDS             complement(3321..3590)
                     /locus_tag="PK754_12060"
                     /inference="COORDINATES: protein motif:HMM:NF028094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HNB10285.1"
                     /translation="MKRHTKIYLDFFGYNISSFIPCEVCGERANDIHHIEARGMGGKN
                     DAADVIENIIALCRKHHIELGDKKQFEDELTDIHFEFMRVNGRYE"
     gene            complement(3909..4352)
                     /locus_tag="PK754_12065"
     CDS             complement(3909..4352)
                     /locus_tag="PK754_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10286.1"
                     /translation="MSKKYYKKQANHESHIRKALAEYLHLQYPNTFWWFDLTGEKMPI
                     GQAKKLSKLKCRKGIPDFFMAEKTSKYNGLFIELKRDDRYVEKIIDDENFGSGKLKEH
                     IEEQRKMIEGLNDRGYYATFAVGFEDAKAVIDRYLSCVVVNETSR"
     gene            complement(4345..4548)
                     /locus_tag="PK754_12070"
     CDS             complement(4345..4548)
                     /locus_tag="PK754_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10287.1"
                     /translation="MPNEQKIEISQEFFKKIITEKLIDIDAELEQVEMDCIAQRDQIN
                     IDRLRVARAIMLDLIDAMWQKNV"
     gene            complement(4567..4950)
                     /locus_tag="PK754_12075"
     CDS             complement(4567..4950)
                     /locus_tag="PK754_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10288.1"
                     /translation="MNLEMIIGILRDYEYLRILIEEKCKVAAKINKRYYISDFLGFSF
                     RLASFDSLSVSTVSDSFSFPLFWLTKSNEELAEIVAAEKKLKEEEYKKVADKFMESIR
                     LNAEHNERELYKKLKEKYEPKPINI"
BASE COUNT         1326 a         1263 c         1008 g         1748 t
ORIGIN      
        1 cgcctgcccc gtgcgcaact tgtattcgcg tatttgttgc ttcatcagat catcttcagg
       61 gacggacaag tcgaactcaa aagataactt actcccgttt ttctgcggga agtatctgtt
      121 aagcatttta gtgaaaaact ggttgtaact tttcaacgac ttattcacgc gattcatctt
      181 gtatagcgcg aattgaatct ttgcctccgc catagtgttc gcatcgggct gtcccgtcat
      241 cacgcgcggc acgccggcca tttggtgaag ctgatcgcgt gttaagttca tcccgttaat
      301 catatcaagc tcaacggcac taaaccctaa cggcgtatat tctgcgtttg tgtccgtccc
      361 gaataaattg cctttacgtg cggcggattt aaagctgcgg ctaaatttag ccatcgattc
      421 ttgaaactta gtttctaaca tagcctcgtt aaacttcaca tatcccggcg gaacaccctt
      481 actcattacc gtttcaaggt tatgctgctt ttgctgttta tcaagattcg ccaagcgtaa
      541 ggccttgttt acccatcccc cgccgccaag actattgccg atgttaggca agcgatacca
      601 taaaatctga tcttccggta tatccataac cttgccgcca ccctgccatt cataccccgt
      661 tgtacgccca taatcatcgc gctttgtgat tagcataccc atgtcggggt atagcggcgt
      721 aaacgattta gggattatcc caccgccctc gccgcgcata gttagcggcg ttatgtaaaa
      781 cggcaatccg tcaataagct gattggcgta ttccattaaa tacatcatgt cgaaaaacgt
      841 aacatcaggg ttgccgtcct caagtaaact aaaagcgtaa tgattgtcta attctttgcg
      901 ctcatcccta ttgataatgt tataaatgga atagttgtcc gtcgcctgcg ctacccaata
      961 agcgtttacg tcgcacaaca aggcaaccca ttcggtattt gtctttaggt tttgctgtac
     1021 atccacacgc gcggtatcat tgaaattagc cgttactatt tggccactta gaccaagcgt
     1081 attgtaaaac ggtctatcgg cggcacgctt agattttaaa tagtacgcac cggctttgaa
     1141 tgattcgtat aagtcacgga agaacccttg tttgtcttgc attttggcca cctaattttg
     1201 ggagagtata gttttagcta tcttttgctt tacgtctata tcgcgcgaac gccgctcttt
     1261 cataattata ctggcggcaa tcgctatctc cctattcgct ttcctatacg aacggaacaa
     1321 gccaatgttt aaacaaaggc tcgtcgttag tagtatgtat agaaattcat tcatcatctt
     1381 ctgctgtccc aaagttagcc gatgttgggt gcaatatacc gctttggcta taccgccatg
     1441 catcggcgca atggttgaat ttatcttcag ggtcgttagt gtatcggttg gtaaatttat
     1501 cgtatttgtt tgaatagttg cgaagttcat gccgcatctc gcttgacctt gccgttacag
     1561 cgtagccgcc cctatatgat tttagatgcg acagccccca gttaatactc cccgcgccct
     1621 tcaccgcctc aactacattg catcctaaat cgcgtaactc ttttattgat ttaggctccg
     1681 cgctatcgca ataaattagt tcgcccgcca atccaagctc tttaagccta cgcgcaatag
     1741 ctgggttgtc aagccgtgtc tcgtaaatca tttgatcggc tataatcata ccgtctaata
     1801 ccgtagagcg aatcaagacg gtcgggtcgt tagtgaatcc aaagtcaaga ccatacgcca
     1861 cgcgctccgc gtctttgggg aaagcgtcca ccacctgtaa gtcggggaat ataagccctt
     1921 cgatggtccc cgttttgccg agaccataga caagccacct atttggattt gttaccttgt
     1981 acgatagaat tttctcaacg attgcagggg gcaaaaaacg gttgtgcgtg aaattagaaa
     2041 tgaacaagtc cacatcatta cgtttacccg cctgcatatc tcttatcatc tcatgcgccc
     2101 aaaattcgtt tgtagggttg tagtctataa tcgtttgctt tgtagtccta acgtaaattt
     2161 cgtcgaacac cgaaaacggt atgccgttgg cctcgttaaa aaaagaatag tcacgctttc
     2221 cagaatgcgc gtcttgtggg tcgtcgaacg atttaaactc aattatagac ccattcgtaa
     2281 aatggtactc acggtttgtt gcgttgtagt caactatata attcttaagc cattcgttgc
     2341 ccttgataat cgtttgcatg tcccgcaatg caccgccacg taaattaggc acatcctgac
     2401 caacgatagt gattgtattc cttgcctcaa tcgccctaat aagtaagtat tggtcaatgc
     2461 catacgtctt accgcttccc gttccgcctt gatgtatgac aataggcttt gcgctattgt
     2521 aggtccagtc aaagatcggc gtagtctcaa aacgtacttc attcatcctt tatttctttt
     2581 tcgtctttta ttgggctaac atttgacgca tttacaatta tagtcggcga tcctccgagt
     2641 atttcgccat ctaagccggt tatttcttgt ctttctatat aacctctttt tttacctttt
     2701 gtttttaaat aaaaaattat cggggcatcg cgtccttttt ctattgattt aaacaattta
     2761 ctttccacaa aatcgagcgc aacattgtct atattttcaa cagccttttt atattttttg
     2821 tcagaagtca tccatttgta atgagtggtt cgatctatgc cgacaacttt agccgcagtg
     2881 gttacaacac ctaaagactt ttccaaagcc tcgagcattg cccttttttg tatgttgaat
     2941 tttgtagcca ttatccaata tattttttga ttcttcacaa ttcagccagc ggcacttcat
     3001 tcggctccgg tatataaacg ccataaaagt tcatcgcaaa cgcacgcatc tgttcataca
     3061 actcctctat cctatgcacc ggttctttct ctattgaccc gccatacttt atttcatccc
     3121 ctccgacgtt gtcaaatttt gggttgaact taatctttag ccagttgttt acaaggtcaa
     3181 ggtcttcttc acccatagct tcagaataag cctttactat tacctcaaag tagtaaccgc
     3241 gctggccttt agacctgccg acttttattt ggtgaccgca tttaggacag ctaatcatag
     3301 gaccgcgctg ttttcttgct tcattcatac ctcccattta cacgcataaa ttcaaagtgt
     3361 atatccgtta attcatcctc gaattgcttc ttatcgccaa gctctatatg atgtttgcga
     3421 cacaacgcta taatattttc tatcacatct gccgcatcat tcttaccgcc cattccacgc
     3481 gcctctatgt ggtggatgtc attagcccgc tctccgcata cctcgcacgg tataaagctg
     3541 cttatattgt agccaaaaaa gtcaaggtat attttagtgt ggcgtttcat aaattacttt
     3601 taagttaata aataacattg acattgtcaa gtgttttttt ccttgacaac gcttaaataa
     3661 cttgacaaac cgcttttgat tcactacatt gttaaaaaat ataaggagag agaatgaacg
     3721 ctaaacaagt cgcttacaaa acaatctata gcataggcaa gttgttattg attttaggat
     3781 tcgccagctt gctctcaaat atgtttgttg ctttggcgtt gacgactata ggcgtgtatg
     3841 atgcgccgta ttggttaaac gtgtgggtat ttattacgcc agtgtgtttg atatgcgggt
     3901 ttttaatatc agcggcttgt ttcgtttacg acgacgcagg ataaatatct atcaatcacc
     3961 gcctttgcgt cttcaaatcc gacggcaaaa gtggcgtaat agcccctatc atttagccct
     4021 tcgatcatct tgcgctgctc ttcgatgtgt tcctttagtt tgcctgaccc gaaattttca
     4081 tcgtctataa tcttttcgac atacctatca tcgcgcttaa gttcgatgaa taacccattg
     4141 tatttagacg ttttttcggc catgaagaaa tccgggattc ctttacggca cttaagcttt
     4201 gatagtttct tagcttgtcc tatcggcatc ttttcgccgg tcaaatcaaa ccaccaaaac
     4261 gtgtttgggt attgcaagtg cagatactct gctaaagcct tgcgaatgtg cgattcgtga
     4321 ttggcttgtt ttttatagta ctttttagac atttttttgc cacatagcat ctattaaatc
     4381 aagcattata gcgcgggcta cgcgcaatcg gtctatattt atttgatcgc gctgagcaat
     4441 gcaatccatc tcaacctgct caagctcggc gtcaatgtct ataagttttt cggtaataat
     4501 tttcttgaaa aactcttgcg atatttctat tttttgttcg ttcggcatga ctactccctt
     4561 tattttttaa atattgatcg gcttaggctc gtatttttcc tttagctttt tgtaaagctc
     4621 acgctcatta tgttcggcgt ttagccttat tgattccata aacttatctg ccactttctt
     4681 atactcttct tctttcaact tcttttcagc cgcgactatt tcagcaagct cttcgtttga
     4741 ttttgtcagc caaaaaagcg ggaaagaaaa actatccgat acggtgctaa cagaaagcga
     4801 atcaaaagat gctaagcgga aagaaaaacc taaaaaatca cttatataat atcttttatt
     4861 tatttttgct gcgaccttgc atttctcttc aattaaaatc cgcaaatatt cgtaatctct
     4921 taaaatccct attatcatct ctaaattcat aacaatctcc tcttattatt agttatacac
     4981 ttgtgttaat taacttctcg gatggtgggg tttcagagca tagttcgctc cttaagtttt
     5041 ttacgctttt gaatagtttt gggaaaaaga acttgcaaag ggttcgttat gttttaggag
     5101 cacttctcgg catggagccg gtcgtcgtcg ctttcgtgtg gtaattgtag gcggaggtct
     5161 gttcccgccg taatctgatc gcgtgcgatg gactatccca tgcccgccaa tcaatgccaa
     5221 ttacccgctc cgctaattgc ttagccaaac cagtcgggcg tttcagccct catttcttcc
     5281 tactttttaa ccgccggtgc gtacctgtca tcagggattc acggggcggc ctttgcaata
     5341 tgtta
//
LOCUS       DAOQNQ010000223         5312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_6295_length_5312_cov_5.617843, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000223 DAOQNQ010000000
VERSION     DAOQNQ010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6295_length_5312_cov_5.617843"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4826
                     /locus_tag="PK754_12080"
     CDS             <1..4826
                     /locus_tag="PK754_12080"
                     /inference="COORDINATES: protein motif:HMM:NF012282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HNB10289.1"
                     /translation="TDTNGDALRIVEILSGFPYHGTVVNGSGDTTIIYTSASEYFGSD
                     SLQYVIADGRGGLDTAAVKITVNSVNDRPDAKDTTTTVTQYNVVRINVLATATDAENQ
                     TLSVSAVSRPKFGDAQIDGTSTSVVYIPNMFYTGVDSFKFYVSDGSLADSATMFVTVT
                     SGDSFLVTWSKFDAGMENWSTVNDVSDSAYRSTGGNPGGYFTATDAVTGAWWYFNAPS
                     KFLGDLSSVYDKTLRYDIKQSPAGTDNSQPDVILIGNGVQLNYDFPANPRTTFTSFGL
                     VFNETAGWKKTSGSVPTQSELQNVLSNVTALRIRGEYASGTDSAGLDNVILEKAQNNN
                     NPVVIMDTEEISEESNITSAVLDNDTDPENHVLHIASISQPRHGTATINSGNLTITYK
                     PDSNFAGVDTVMYYAADNYGGQSFGYLQITVNNINDAPTISSAVRDTAYNEDFGKVYV
                     ARLLDIFGDVDNGLTFGGSTIASGATPYISNDSLYLLSVASFNGIATIELTANDGEYT
                     IRDTFSVDITMQNDAPYVASAIRDTTLAEDFGRIFYVNLSDHFADIESDSLIYTIMTI
                     ATGVTAHIQHDSLYVMSVNDFHTATATLSITAGDGQYSVSDTFDVNVTPVNDPLVQTT
                     ALNNQTLIENFDKTVLGYLPNHFSDPDDATLSYTASVMSGNVVAYISGDTLYAESVAG
                     FLGDAFLRITASDTQYTVADTLTVSVVPFNYTPTVVQPLRDTTWSEDIGKIFIAELNS
                     HFTDVESSTLIYSGQVIAGSGTTTITNDSLYLQSAKDFYGNILVRITASDGALSVSDT
                     LAVEITNVNDAPQIASHISDITKTVDFGKVYVEKGSNVFNDVDNDVLTMTATALQAGV
                     NTEVSGDSLYIISIGGFMGSVGILVTAHDAEYSVTDTFYVTVNPENFPPTLVQNLRDT
                     TFAEDFAKTRVYRLNSFFNDVDDINLSYSSQILSSGVSAIIAQDSLYLSGVADFNGIA
                     VVRIQAQDSYGNSVADTINVIVTPVNDAPNRTHQITDMDLAQDFGKAYVDKLSAVFND
                     IDNGTLNYAVSTLNAGVNGLIQNDTLYLISTNGFTGASTLRITASDGEYTVADTLIVN
                     VFSTNHSPVLAQMLRDTTLNEDFGRIRHSVLTQFFTDSDLDALTFTATAETGVSTSIQ
                     NDTLYLTSTSQFSGTPKVIVSVSDGLVAISDTFTVTINSVNDAPSAFTLVTPVNNDLL
                     TSLRPAFVWRKAIDQDGDVLSYSIEVSTNNTFASTVINTSTSDTSYAPASDLDSAQSY
                     FWRITAHDGHGGNTVSNVQSFTVDAKRPTLSLWFNQGPILKSYIDLQVYSSEVLSGAV
                     QAQVTMKNTANTVTDNRTVSLQRLGAGTLYTGKYQMNTEGSLTVAINAQDAAGNTRSV
                     QRIYQVAAVSKSHTLALKSTSVLLSSNGRMKSSDGFILMGEDTEGELTEILVSTSVNI
                     HGTIEISDALNLVIRLDKEMLAEASVQSQFDKRQIGIYRKTDMGYEYVGGQSDGYEVR
                     AKIAHYGTYVALYRAGHVIVPDRLELAQNYPNPFNPSTTIEFGLTENGNVKIAVYNLL
                     GQLVKTLVDEKREAGYHKIVWDGRNAAGQLSASGVYIYRLETKSGFVTRKMMLIK"
     gene            4849..>5312
                     /locus_tag="PK754_12085"
     CDS             4849..>5312
                     /locus_tag="PK754_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10290.1"
                     /translation="MKRSKLFMGLVAGALIFNACGPAEADPDAIEKAWTLYEEGNVSE
                     AKIIFAEQSAIGNAEAFVGLGWCAIDENQAATAHTYFQTVSGDSLSDAYAGWCAVSWS
                     LEDYPSAIMYAQFVLRHDNAYTFSHKSSVTHSDLIWYQASSYLHASNYSQCYA"
BASE COUNT         1422 a         1163 c         1280 g         1447 t
ORIGIN      
        1 atacggatac caatggtgac gcgcttcgta ttgtcgaaat tctctccgga ttcccttatc
       61 acggaaccgt ggtaaatggt agtggcgata cgacgattat ttatacgtcc gcttcggaat
      121 atttcggttc ggatagtttg cagtatgtga ttgctgacgg ccgcggcggt ttggatacgg
      181 cggcggtaaa aatcacggtt aacagtgtta atgatcgtcc tgacgcaaaa gatacaacaa
      241 cgacggtcac gcaatataat gtcgtccgca ttaatgtact ggcaacagcg acggacgccg
      301 aaaaccaaac gctctcggtg tcagcggtat cacggcctaa gtttggtgac gcacagattg
      361 atggtacatc cacctctgtt gtgtatatac caaacatgtt ttataccggc gtggatagtt
      421 tcaaatttta tgtctccgac ggatcattgg cggattcggc cacgatgttt gttacagtta
      481 catccggcga ttcattcctt gtcacgtgga gtaagtttga cgcgggtatg gaaaattggt
      541 caacagtcaa tgatgtatcg gattccgcat accgttcgac cggaggaaat cccggcggct
      601 attttacagc aaccgatgcc gtaacaggcg cctggtggta ttttaacgca ccttccaaat
      661 ttctcggcga cctcagctcc gtttatgata aaacactgcg ttatgatatc aaacaaagcc
      721 ctgcaggaac ggacaattcg cagcccgatg tgattctgat cggtaacggt gttcagctaa
      781 attatgattt tccggcaaat ccgcgtacta cgttcacgag ttttggtttg gtgttcaacg
      841 aaacggcggg atggaaaaaa acatcagggt ctgttccgac acaatctgaa ttgcaaaacg
      901 tactgtccaa tgttacagct ctgcgtattc gtggtgaata tgcttcaggg acggatagcg
      961 ccggtttgga taatgttatt ctggaaaaag cgcagaataa caataatcct gttgtcatca
     1021 tggataccga agaaatttca gaagaaagca acataacatc ggctgttctt gataatgata
     1081 ctgacccgga aaatcatgtg ctccatattg cttcgatttc ccaaccgcgg catggcacgg
     1141 ctacgattaa ctcgggtaat ctgacgatca catacaaacc ggattccaat tttgccggtg
     1201 tggatactgt gatgtattat gcggcggata attacggcgg acagtccttc ggctatctgc
     1261 aaatcacggt taataatatc aacgatgcac cgaccatttc gtcagcggta cgtgacacgg
     1321 cgtacaatga agacttcggc aaagtttatg tggctcgctt attggatatt ttcggtgacg
     1381 tggataatgg tctgacgttc ggcggaagta cgatcgcatc cggtgcgaca ccgtatattt
     1441 ccaatgacag cctctatctc ttaagcgttg cttcgtttaa cggtatcgca acgatcgaac
     1501 ttacggcgaa cgacggtgaa tatacgatcc gtgatacttt tagtgtagat atcacgatgc
     1561 aaaatgatgc accgtatgtc gcatccgcga tcagggatac gacccttgca gaggattttg
     1621 ggcgaatttt ttatgtcaat ctatctgacc attttgcgga tatagagtct gactcgttga
     1681 tctatacgat aatgaccatc gccacaggtg taaccgctca catacagcat gattcgttat
     1741 atgtgatgag tgttaatgat tttcatacgg ccacggctac actaagtatc acagcgggcg
     1801 acggacagta ctctgtatcc gatacgtttg atgtaaatgt aacgccggtc aacgatccgc
     1861 tcgtgcaaac gacagcgctc aataaccaaa cgttgatcga aaattttgac aaaacggttt
     1921 taggttattt accaaaccat ttttctgatc cggatgatgc gacgctttca tataccgcgt
     1981 ccgtaatgag cggtaatgtt gttgcctata tttccggtga tacgttgtat gccgaaagcg
     2041 tagccggatt cctcggtgat gcgtttttgc ggatcacggc ttccgatact caatatactg
     2101 ttgcagatac gttgacggtt tctgttgtcc cgttcaacta tacacccacg gtggtgcaac
     2161 cgttgcgcga tacgacgtgg agcgaggata tcggaaaaat atttatcgca gagctcaatt
     2221 cgcacttcac agatgtcgaa tcttcgacgc ttatttatag cggtcaagtt atcgccggta
     2281 gcgggacaac aacaatcact aatgatagtt tgtatttgca aagtgcaaaa gatttttacg
     2341 gtaatattct tgttcgcata acagcctcgg acggtgcact atccgtcagc gatacgttgg
     2401 ctgtagagat aaccaatgtg aatgatgcgc cgcaaatcgc atcgcatata tcagacatca
     2461 ctaaaacggt ggattttgga aaagtatatg tcgaaaaagg ttcgaatgta tttaacgatg
     2521 tggacaatga tgtgcttacg atgacagcga ccgctttgca agcgggtgtc aatacggaag
     2581 tctccggtga cagtctttat atcatcagta ttggtggttt tatgggtagt gtgggaatcc
     2641 ttgtaactgc acatgacgcg gaatattcgg tgaccgatac attttatgtc accgtcaatc
     2701 cggaaaactt cccgccgacg cttgttcaaa atttgcgcga taccacattc gccgaggatt
     2761 tcgccaaaac gcgggtgtac cgattaaaca gctttttcaa tgatgtggat gatattaatc
     2821 tgagttattc atcgcaaatt ctttcttccg gcgtttccgc gatcatcgca caagattcgc
     2881 tatatcttag cggagtggcc gattttaacg gaatagccgt cgtgcgtatc caggcgcaag
     2941 atagttatgg aaacagcgtc gccgacacga tcaatgtgat cgttacaccg gtcaatgatg
     3001 cgccgaaccg tacccatcag atcacagaca tggatttagc acaggatttc ggtaaagctt
     3061 acgttgataa attatcggcg gttttcaatg acatagataa tggaacactc aattatgccg
     3121 tatcgacgct caatgccggg gtcaacggcc tgattcaaaa cgatacgctg tatcttatca
     3181 gtacaaatgg ttttacaggc gcgtccacgt tacgtatcac ggcatcggac ggcgaatata
     3241 cggttgccga taccttgatc gtcaatgtgt tttctaccaa tcattcgcct gtacttgctc
     3301 agatgctgcg cgatactacg ctcaatgaag attttgggcg catacgtcac agtgttttga
     3361 cgcagttttt tacggattcg gatcttgatg cgttgacctt tacggcgacg gcggaaacag
     3421 gcgtttcaac ttccatacaa aatgatacac tgtaccttac aagtacttcc caatttagcg
     3481 gtacgccaaa ggttattgta tcggtttcgg atggcttggt agcgatttcg gatacgttta
     3541 ccgtcacaat taattctgtt aacgatgcgc cgtcggcgtt tactttagtc acgcccgtaa
     3601 ataatgacct gcttacatcg cttcgtcctg cttttgtttg gcgcaaagcc atagatcagg
     3661 atggtgatgt gctgtcatac tcgatagagg tatccacgaa taatacgttt gcttctacag
     3721 tgatcaatac ttcgacgtcc gatacgagct acgcaccggc atccgatctg gacagcgcac
     3781 agtcgtattt ctggcgcatc acggcgcatg atggccatgg cgggaacacg gtatccaacg
     3841 ttcaaagttt tacggtagat gccaagcgcc caacgctcag tctttggttc aatcaaggcc
     3901 cgattttgaa atcatacatt gatttacagg tttattccag cgaagtactc tcaggagctg
     3961 tgcaagcgca agtaacgatg aaaaacacag caaatacggt tacggataat cgtaccgtat
     4021 cactccagcg tttgggcgcg ggtacgcttt ataccggtaa atatcaaatg aatacggaag
     4081 gcagccttac tgtagctatt aacgcacaag acgcggccgg caatacgcgt tcggttcagc
     4141 gaatttatca ggttgctgca gtttcaaaat cacacacact ggcactgaaa tcaacttcgg
     4201 ttttactttc gtctaatggg cggatgaagt cgtccgatgg atttatcctc atgggtgaag
     4261 atacggaagg ggaattaacc gaaatattgg taagtacgtc tgtaaatatc cacggtacga
     4321 tagaaataag tgatgcatta aacttagtga tccgacttga taaagagatg ctcgcagagg
     4381 catccgtgca atcgcaattt gacaaacgac aaatcggtat ctatcgaaaa accgatatgg
     4441 gatatgaata cgttggcggt caatcggacg gatacgaggt gcgtgcaaag atcgctcact
     4501 atggtacata cgtcgcgctg tatcgcgcag ggcatgtcat tgtgccggat cgtctggaac
     4561 tcgcgcaaaa ctatcccaat ccgtttaatc cgtccacgac gattgagttt ggccttacgg
     4621 aaaacggaaa cgttaaaatc gcagtgtaca atcttttagg tcaactcgtc aaaacactgg
     4681 tggacgaaaa acgcgaagcc ggttatcata agatcgtatg ggatggccgt aatgcggcag
     4741 gtcagctttc agcaagcggt gtgtatattt accgtcttga aacaaaaagc ggatttgtca
     4801 cacgtaaaat gatgttgatc aaataagaaa aaacaggaga tatcatatat gaaacgttca
     4861 aaattattca tggggctcgt tgccggtgcc ttaatattta atgcttgtgg ccctgccgaa
     4921 gcggatcccg atgcgatcga gaaagcatgg actctgtacg aggaaggaaa tgtttcggaa
     4981 gcgaaaatta ttttcgccga acaatccgcc ataggcaatg ccgaagcgtt tgtaggtttg
     5041 ggttggtgtg cgatagatga aaatcaggcg gcgacagcac atacatattt tcagaccgtc
     5101 agcggtgaca gcttaagcga tgcgtatgcg ggctggtgtg ctgtgagttg gtcgcttgag
     5161 gattatccat ccgctataat gtatgcgcag tttgtgcttc gtcatgataa tgcctataca
     5221 ttttcacata aatcatccgt tacgcatagc gatctgattt ggtatcaggc gtcttcatat
     5281 ttgcatgcgt caaattattc gcaatgctat gc
//
LOCUS       DAOQNQ010000224         5284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_6355_length_5284_cov_5.380379, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000224 DAOQNQ010000000
VERSION     DAOQNQ010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6355_length_5284_cov_5.380379"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PK754_12090"
     CDS             <1..504
                     /locus_tag="PK754_12090"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HNB10291.1"
                     /translation="SSTLFRTVFFAGLNVKKRTNHSYAVKYYEPLGLDATIFIPAPDL
                     VFENDTKNTIIIQNLVDLKRTKMYFKLFGKKDGRKVEYEGPIDQGTVGEKNEHYRVMW
                     TRSVELPDGTKRTDKFGSTYKNKDLVKKHKPDSTLHNSADSTGLRNGVVFGDSAHTGA
                     TTSNTPN"
     gene            complement(501..1601)
                     /gene="mutY"
                     /locus_tag="PK754_12095"
     CDS             complement(501..1601)
                     /gene="mutY"
                     /locus_tag="PK754_12095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNB10292.1"
                     /translation="MTSQRTKTQTPEKITKNLLLWFYKHKREMPWRTTQDAYRIWVSE
                     VMLQQTQVEQVRPYYQRFMVAFPDILTLANAKLDEVYKLWEGLGYYRRAALLHKAAQI
                     VVTQYQGRIPDQYEFARSLPGFGDYTIGAVLSIAYQKKLPAIDGNVIRVMSRLYRIES
                     EVHLSATKRQIESLVQSMIPDENPGDFNQALMELGALICTPQSPQCTRCPVHRFCKSY
                     NELPNPNILPNKKKKTIKPHMHIAAGIVFKNKRILAAKRPSDVILGNLWEFPGGKKES
                     GESLLKTCERELQNKIGIRAKIGKCFLVHKHAYSHYTITLHFYKCDYIGGQPIKRKHS
                     ALRWVYPKKALELAWATSHRKALEKLLKDAIY"
     gene            1713..2270
                     /gene="dcd"
                     /locus_tag="PK754_12100"
     CDS             1713..2270
                     /gene="dcd"
                     /locus_tag="PK754_12100"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNB10293.1"
                     /translation="MILTDTQILAEIAKGTIRIEPFRRDCLGSNSYDVHLGKYLAVYS
                     EETLDCKKDNPIEHFEIPEEGYVLMPTKLYLGVTQEYTESMAHVPFLEGKSSIGRLGI
                     DIHATAGKGDIGFCNTWTLEISVKHPVRVYEGMPIGQLIYFEVSGQIETPYNSKPSAK
                     YNERTIRPVASMMFKNFDREKNRWK"
     gene            2287..3840
                     /locus_tag="PK754_12105"
     CDS             2287..3840
                     /locus_tag="PK754_12105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB10294.1"
                     /translation="MMAEQQMYSLSEVLNALRMRAKPETFASVWLQEWLRQCDVVRLE
                     CFGTNNDGQWSSILRWEKNGTHNPALNSQPAHPNAAMQIAFKQAEPLIGDSSQIYGKA
                     SAVPMTSIVLPVLSDKQVVGLIVADKLTEPEKSINIPFLELLSSLWGLHRASLDATET
                     LETVRFERDNLRAQIEQTLAAVKPETPQDELARTMITIARSLAQMLLPSITQQDKNNI
                     DAVLAFSKEVSMYEKNELHTTYSESQDNAESDISSVLDESVDIARNDETCVKKRFIID
                     FKRQDREIVWSEFKRLQQFISSVFSLMIGYGKENSTITVEYTPHGEFLKILIKVDSER
                     EFNEKIGDFYTFTARLNGWECDWSAHENRIQCILSLRRITGSDSSKKNSQSLSDPKKE
                     TRLLIVDDDESLRELMVDILESRNYEVVCCPDASSAMEQINKLRYDMIITDLGLPNIS
                     GSELAAQIKALNPHLPVMMMTGWNIDTERPDARTTYVDFILPKPFNLNELLEKVENGL
                     HASRTMRHS"
     gene            3837..4979
                     /locus_tag="PK754_12110"
     CDS             3837..4979
                     /locus_tag="PK754_12110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB10295.1"
                     /translation="MKPTLMLVDDESRVLEVLVKTFESQYQIFSFTGAHDALELLKRQ
                     PVQVIITDERMPKMNGMAFLKEAKVLAPNTVNIILTAYTDINVAIEAINSAVVYRYVV
                     KPWDTDDLRAIVRQAFERYALVQENKKLTDELITKNAALEQNLKQLKETQEKLLRQEK
                     LAVVGQLTASIGHELRNPLSRIKASAALMRNEILPEEKEKKELLQIIDNEVMISTKII
                     NDLLDYSRDRKTIFKPGNFNIIIDETLMRMRFPDEVEVVRELAPDLPDNQVDEGQIQQ
                     ILINLMLNAIQAMEYSGKIYVRTHHDGDAAIVEIKDTGCGISNENLERIFEPLFTTKP
                     KGIGLGMCIVKMLIEKHGGTIGITSRENIGTTVKIRIPFHPNFMEN"
     gene            4976..>5284
                     /locus_tag="PK754_12115"
     CDS             4976..>5284
                     /locus_tag="PK754_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNB10296.1"
                     /translation="MMFLPIADQLAVIKRGIVDLIPEDELVKKLENSLRTAKPLKVKL
                     GCDPSRPDLHIGHSVVLRKLRQFQDLGHQAILVIGDFTGMIGDPTGKNKTRPSLSLEE
                     T"
BASE COUNT         1607 a         1017 c         1158 g         1502 t
ORIGIN      
        1 tcaagtacac tttttagaac ggtttttttt gccggattga atgtcaaaaa acgtacgaac
       61 cattcgtatg ctgttaaata ttatgaaccg cttgggctgg atgcgacgat ttttataccg
      121 gcaccggatt tggtatttga aaacgatacc aagaatacga ttatcattca aaatcttgta
      181 gatctcaaac gaaccaaaat gtatttcaaa ctttttggga aaaaggacgg acggaaagtt
      241 gagtatgaag gcccgataga tcaagggaca gtcggcgaaa aaaatgaaca ctaccgcgtt
      301 atgtggacgc gttcggttga gttgccggat ggaacaaaac ggacggacaa attcggctcg
      361 acatacaaaa acaaagacct tgttaaaaaa cataaaccgg attcgacact tcataattct
      421 gcggacagta ccggattacg aaatggggtt gtttttggcg attcagccca caccggtgcg
      481 acgacgtcca atacacccaa ttaataaatc gcatccttta gcaacttctc taatgctttc
      541 cggtggctgg tagcccatgc caattccagt gcctttttgg ggtaaaccca acgtaaggct
      601 gagtgtttgc gtttgatcgg ttgacctcca atgtagtcgc atttatagaa atgtaatgta
      661 atggtataat gtgaatacgc atgcttgtgt actaagaagc atttaccgat tttggcccta
      721 ataccgattt tgttttgcaa ttcgcgctca caagttttta aaagcgattc accggattct
      781 tttttccccc ctggaaattc ccatagatta cccaaaatca catcggacgg gcgtttagcc
      841 gcaagaattc ttttattttt aaaaactatt cctgcagcga tatgcatgtg aggtttgatg
      901 gttttctttt ttttgttagg taaaatatta ggattgggta attcattata agatttgcaa
      961 aatcggtgta caggacagcg ggtgcattga ggtgattggg gcgtgcatat caaagcgcct
     1021 agctccatga gtgcttgatt aaaatcgccc gggttttcat ccggtatcat ggattgcact
     1081 aaactttcaa tttgtcgttt ggttgcggac agatgaactt cagattcgat tcggtaaagc
     1141 cgactcataa cacggattac gtttccgtca atggccggta atttcttttg gtacgcgata
     1201 ctaagtacag cgccgatagt ataatctccg aaaccgggta agctacgtgc aaattcgtat
     1261 tgatccggta tacgaccttg atattgagta actacgatct gagctgcttt gtgaagtaaa
     1321 gcggctcgac ggtaatagcc taaaccttcc caaagtttat aaacttcgtc aagtttagca
     1381 ttggcaagcg ttaaaatatc agggaatgcg accataaatc tttgataata ggggcgcact
     1441 tgctccactt gagtttgttg aagcatgact tccgaaaccc atatgcgata ggcatcctgt
     1501 gttgtacgcc agggcatctc tcgtttatgt ttatagaacc acaggagaag attttttgta
     1561 attttttccg gcgtttgggt tttagttcgt tgagacgtca tggatagtgt tggaggtaaa
     1621 ttatttattg ataaacgctg tagtgttgtc tatatttatt tctaatattg gattcattta
     1681 gttttattac aatcctaatt ttatttatcc caatgatctt aacggacaca cagattttag
     1741 ccgaaatcgc taaaggtacc atacgtattg aaccttttcg ccgcgattgc cttggttcca
     1801 atagttatga tgtgcattta ggcaaatacc tcgcggtgta ttccgaggaa actctagatt
     1861 gcaaaaaaga taatccgatc gaacattttg aaatacccga agaagggtat gtattgatgc
     1921 ccacgaaact ttatcttggt gtaacccaag aatacacgga gtccatggcc catgttccgt
     1981 ttttggaagg caaatcaagt atcggaagat tgggaattga cattcacgcg acagccggta
     2041 aaggggatat tggtttctgt aatacatgga cactcgaaat ttcagtaaag caccccgtac
     2101 gcgtatacga gggtatgccc ataggtcaat tgatttattt tgaggtttcg gggcagattg
     2161 aaacacctta taattctaag ccgtcggcta agtataatga acgcacgata cgtcctgtgg
     2221 catccatgat gtttaaaaat tttgatcgtg aaaaaaatcg ctggaaatga aagagccgat
     2281 tataacatga tggctgaaca acaaatgtat tccctctccg aagtattgaa cgcattgcgt
     2341 atgcgtgcga aacccgaaac ttttgcatcg gtatggttac aggagtggct ccgtcagtgc
     2401 gatgttgtgc gattggaatg ttttggaaca aacaatgacg gtcaatggag ctctattctg
     2461 cgatgggaaa aaaatggcac tcataatccg gcactaaatt cacaaccggc tcatccgaat
     2521 gccgcgatgc aaatcgcctt taagcaagca gagcctctaa tcggagatag ttcccaaata
     2581 tacggtaaag catcagcagt gccgatgaca tcgattgtat tgcccgtttt atcggacaaa
     2641 caagtagtag gccttattgt tgccgataaa ttgacggagc ctgaaaaaag cataaatata
     2701 ccttttttgg aattattgtc atcgttatgg ggcctgcacc gcgcctcttt ggacgcaacg
     2761 gaaactctcg aaaccgttcg gtttgaaaga gataatttgc gcgctcagat tgaacagact
     2821 ctagccgccg ttaagccgga aacgccacaa gatgaattgg cgcgtacaat gattacgatt
     2881 gctcgaagct tagcacaaat gctgctaccg tcaataactc aacaagacaa aaacaatata
     2941 gatgctgttt tagctttctc aaaagaagtt tccatgtatg aaaaaaacga attgcataca
     3001 acttattctg agtcgcagga taatgcagag tccgatattt catcggtctt agacgaatct
     3061 gtagatatcg caagaaacga tgaaacgtgt gtcaaaaaaa gatttattat agacttcaaa
     3121 aggcaagatc gtgaaatcgt ttggtctgag tttaagcgat tgcaacaatt tatatcttcc
     3181 gtattttctt tgatgatcgg ttatggcaaa gaaaattcga caataactgt tgaatacacg
     3241 cctcatggtg agtttttaaa aattctaata aaggtcgatt ccgaaagaga gtttaacgag
     3301 aaaatcggtg atttttatac tttcaccgct aggttaaatg gctgggaatg tgattggtca
     3361 gcacatgaaa acaggataca gtgtattcta tcgctgcgac gtattacggg ttcggattca
     3421 tcgaaaaaaa actcacaatc tttgtccgat cctaaaaagg aaacaaggct tctaattgtt
     3481 gatgatgacg aatctttacg tgaacttatg gtggatattt tggagtcgcg taattatgaa
     3541 gtcgtatgtt gcccggatgc atcgtcggcg atggagcaaa ttaataaatt acgctatgac
     3601 atgatcatta cggatttagg tcttcccaat atcagcggct ctgaattggc tgcacagatc
     3661 aaagcgttaa acccacatct gcctgttatg atgatgaccg ggtggaatat cgatacagag
     3721 cgtccggatg cgcggaccac atatgtagat tttattttgc cgaaaccttt caatttgaat
     3781 gaattgctgg agaaagtaga aaacggtctc catgcatcac gtacgatgag gcattcatga
     3841 aacctacttt aatgctggtt gatgacgaat caagggttct ggaggtactt gttaaaacat
     3901 tcgaatccca atatcagatt ttttctttta ctggagctca tgatgcgctg gagttactca
     3961 aaaggcaacc ggttcaggtg atcattactg atgaacgtat gccaaaaatg aatggcatgg
     4021 cgtttcttaa agaagcaaaa gtattggctc ccaatacggt gaatatcatc cttacggcct
     4081 atacggatat caatgttgct atcgaagcta tcaatagtgc agtggtatat cgttatgtcg
     4141 taaaaccatg ggatacagat gaccttagag cgatcgtacg tcaagctttt gaacggtatg
     4201 cattggtaca agaaaataaa aaactaactg acgagctaat cactaaaaat gcggcactgg
     4261 aacaaaacct gaagcaatta aaggagactc aggaaaaact gttacgtcag gaaaaattag
     4321 ccgtagtggg tcaattgacc gcgagtatcg gccatgagtt acgcaacccg ctgtcgcgta
     4381 tcaaagcgtc cgctgcgttg atgcgaaatg aaattttacc cgaagaaaaa gagaaaaaag
     4441 aacttcttca aatcattgat aatgaggtca tgatatctac caaaattatt aatgaccttt
     4501 tagattattc ccgtgaccgt aaaactattt ttaaaccggg caattttaac atcattattg
     4561 atgaaacgct aatgcgcatg cgtttcccgg atgaagtgga ggttgttcgc gaattggcac
     4621 ccgatctacc ggataatcaa gtggatgaag gtcagattca gcaaatactt atcaatctca
     4681 tgcttaatgc catccaggcc atggaatata gcggaaaaat ctatgtccgt actcatcatg
     4741 acggagatgc cgcaatcgtc gaaatcaaag ataccggatg cggtatatcc aacgaaaacc
     4801 ttgagcgtat tttcgaacct ctttttacta ccaaaccgaa aggaataggc cttggtatgt
     4861 gtatcgtaaa aatgttaatt gaaaagcacg gaggtaccat cggtattact agcagagaaa
     4921 atatagggac taccgtaaag atcagaattc cttttcatcc gaattttatg gagaattgat
     4981 gtttcttccg attgctgacc agcttgcagt tatcaaacgt ggcatcgttg acttaatccc
     5041 agaagacgaa cttgtaaaaa aacttgagaa ttctctcaga accgcaaaac cgcttaaagt
     5101 caaattaggc tgtgatcctt cacgtccgga tttacatata ggccattctg tggtattgag
     5161 aaaattacgc caatttcaag atcttggaca tcaggcgata ttggtgatcg gtgattttac
     5221 cggtatgatc ggtgacccaa caggtaaaaa taaaaccaga ccatcgttat cattggagga
     5281 gaca
//
LOCUS       DAOQNQ010000225         5275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_6378_length_5275_cov_5.322797, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000225 DAOQNQ010000000
VERSION     DAOQNQ010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6378_length_5275_cov_5.322797"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PK754_12120"
     CDS             <1..491
                     /locus_tag="PK754_12120"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB10297.1"
                     /translation="RMQSLAKVPNGLYVNDALSFILLLQESKKNLSFQKALAKSDLLT
                     EQRKYTEALRELNHISETLEESRLKEWVLMRIGITHERLGQYNEAVSTFYQVLTAEPQ
                     SIQADLALKKAAEIYDYKLNRPDKAIQLYRQLLVKFPKSIFQNEIRKRLRLVEQALGG
                     QS"
     gene            complement(528..1361)
                     /locus_tag="PK754_12125"
     CDS             complement(528..1361)
                     /locus_tag="PK754_12125"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB10298.1"
                     /translation="MDHTFADACAKLAETYYRASLQHKTNKNTKMHTAMLDQSKRFML
                     MAFDAQASNGNAHFVRGLLAFDNKAYDEALRDFEHAEMYGYKSFELHSMMAYAYNEKE
                     KTALCVEQYQKAIAIKPEDMNTLYNLGELYFQLGNYNMASDYFSKMVKIDPTHSQARI
                     NYALSVWKNGNPNLGKSMINQLLDTDGKTPVNLYNAIAWQLIDKDIDIAMGLKLAQAA
                     NGMKPNNVESIDILGWGYYKNGEYTKAVEYLSQSMKKMPSDEVKNRLKMAKDKLEESK
                     K"
     gene            complement(1604..4330)
                     /gene="polA"
                     /locus_tag="PK754_12130"
     CDS             complement(1604..4330)
                     /gene="polA"
                     /locus_tag="PK754_12130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNB10299.1"
                     /translation="MSERLFLLDGMALAYRAYFAFIHNPLKNAKGENTSAVFGFINAL
                     IKIRDEEKPDYIAVAFDLAEPTFRHKQYSEYKATREKMPEDMVPQLHYLKELTEAMGV
                     PILEYPGYEADDVIGTLSVRAAQQGIVAHMVTGDKDFMQLIGPNIVMYNPLKKGGEKE
                     IIGRDGVLEKFGVEPSQVTDILGLMGDAADNIPGVKGIGEKTAAKLINQYGSIEKIYE
                     QIDSIKGKLRENLEQHKEDAFLSKSLATIALDVPLREDIHDLRGRTPDHQKVTRLLTE
                     LEFKSLIKKILKNESDLFVEDTPLQHERTPEPARNYHTLQTEVAIKELVTKLTNSGGF
                     AWDTETTDADPMRAELVGFSFSWTEGEAFYIPCNGDVPVDTICTLLKPVLEDSTIPKW
                     GQNTKYDIIIVSHYGIEVQGTAFDTMIAGHLLHSDKEVNIDALAASYLGIEKIPTTDL
                     IGTGKNQITMRDVPIEKVSAYANEDADCVVRLKNVMSPLLKETHSAKIFNTIEMPLIP
                     VLQRMERHGVWLDLEILSTMSKDFAIQIIQEEQRIFKEAGTDFNMNSPQQLGEILFEK
                     LQIHKLAGIEKPKLTGKTKQYATDVKILEQYRTLPIVEAILNYRQLTKLKSTYIDGLP
                     PLRNPRTGRIHTTFNQTIAATGRLSSTNPNFQNIPIRTDMGREIRRAFTAQEKGWVLL
                     SADYSQIELRVMAHMSGDENLIAAFSEDADIHTSTAARVFGVEANEVTKDLRRKAKDI
                     NFGVIYGISPFGLAQRIGMSQAEAREFIHSYFATYPKVKSYIESVIAKGKEDAFVTTL
                     FGRRRYLPDLQSKNFTVRQFAERAATNTPIQGTAAEIIKMAMLKTHAALEKSSLSAKM
                     VLQVHDELVFETPQAELQQLEALVRSSMESAVQMKVALKVDMGSGDNWLETK"
     gene            complement(4370..5011)
                     /locus_tag="PK754_12135"
     CDS             complement(4370..5011)
                     /locus_tag="PK754_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10300.1"
                     /translation="MSLKLPWDIESLHLICERDEESWLHGGTHYFHENNSSILAVAHL
                     DCVQAYAGFDFEETDGHSTVHCTTLDDRLGVYIITELLPSIGVTADVLLSTDARHGES
                     TAQMFIPEKKYHWIFSFDRKGSGVVLYQYGDAEILQLMQKYGFEVHEGYRSDIVLMDH
                     LECKAFNFGCGYHEPLTDKSFAVLDESEVNILRFQQFYNDHKNTLMPHVPSYL"
     gene            complement(5008..>5275)
                     /locus_tag="PK754_12140"
     CDS             complement(5008..>5275)
                     /locus_tag="PK754_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10301.1"
                     /translation="SIGNVSWWENIIQAYRAHPEKIIEEDGFASVITSAGDTVLYQRY
                     NDGKWYCLLPVHTFWKTLATVSRQNLDVTRRNSIVFSSALRDTL"
BASE COUNT         1410 a         1128 c         1105 g         1632 t
ORIGIN      
        1 agcggatgca atctttggct aaagtaccta acggtttata tgtcaatgac gcgctttctt
       61 tcattttact tttgcaagag agtaagaaaa atttatcgtt tcagaaggct ctcgcaaaat
      121 ctgatttgct gacagagcaa cgaaagtata cagaagcttt gcgtgaactc aaccatatca
      181 gtgaaacact agaggagagt cgcctaaaag agtgggtttt gatgcgtatt ggaataaccc
      241 acgaacggct cggtcagtac aatgaagctg tgagtacgtt ttatcaagtt ttgacagctg
      301 aaccccaaag cattcaggct gatttggctt taaaaaaagc ggccgaaatt tacgattata
      361 aattaaatcg tcccgataag gcgattcagc tctaccgaca attgttagta aaatttccaa
      421 aatcgatatt tcaaaatgaa ataagaaaac gcctgcggtt ggtagagcag gcgttaggcg
      481 gtcaatcgta gaattttaaa ttatgggttc tagtttgttt ttaatattta tttttttgac
      541 tcttcaagtt tatctttagc catttttaat ctgtttttca cttcatccga tggcattttt
      601 ttcattgatt gggataggta ttctaccgct ttggtatatt caccgttttt atagtagccc
      661 caacctaaaa tatcaattga ttcaacatta ttaggtttca ttccgttagc ggcctgagct
      721 aatttgagtc ccattgctat gtcaatatct ttatcgataa gctgccaggc aatggcatta
      781 tataaattca ccggtgtttt tccgtcggta tccagtagtt ggttgatcat agattttcct
      841 aagttcggat ttccgttttt ccaaactgac aaagcatagt taatacgtgc ttgtgaatgt
      901 gtcggatcta ttttaaccat tttgctaaaa taatcggatg ccatattata gtttccaagt
      961 tggaagtata attcgcctag attatataat gtgttcatat cctccggctt tatagcgatg
     1021 gctttttgat actgttctac acaaagagcc gttttttcct tttcattgta tgcataagcc
     1081 atcatgctat gcaattcgaa gcttttgtaa ccatacattt ctgcatgttc aaaatctcgt
     1141 aacgcttcat catatgcttt attatcaaaa gccaataaac cccttacaaa gtgtgcattg
     1201 ccattagaag cttgagcatc aaacgccata agcataaagc gtttggattg atcaagcata
     1261 gcagtatgca tttttgtgtt tttgtttgtt ttatgttgaa gggaagcgcg ataatatgtt
     1321 tctgccaact tggcacatgc atccgcaaag gtatgatcca aatccacggc cttagtgaag
     1381 agatttgcgg cggcttcgat acgaccggca tctaacgagt ccataccttc tttataatac
     1441 tttacggccg tacgggggac gcccggatat tctttagaac gtaaaaaaaa gtaccaaatt
     1501 gccaatccca cgagtattaa tccgcatacc gcaatgatta ttgttttgta attgatctta
     1561 cttgctttca cggaagccat attgtctcct tgttatatca attttatttt gtttctagcc
     1621 aattgtcgcc gctgcccata tccaccttaa gtgcaacttt catctgaaca gcagactcca
     1681 tggacgaacg aactaatgct tcaagttgct gtaattcggc ctgtggtgtt tcaaatacca
     1741 attcgtcatg aacctgtaaa accatttttg ccgaaagtga acttttttca agcgccgcat
     1801 gtgttttgag catggccatt tttatgatct cagcggccgt tccttgtatc ggcgtattgg
     1861 tcgctgcgcg ctctgcaaat tggcgtaccg taaagttttt actttgtaag tccggtaaat
     1921 accgtcggcg accaaagaga gttgttacaa aggcgtcttc ttttcctttg gcaataaccg
     1981 attcgatata cgactttact ttaggatatg tagcaaagta actatgaata aactcgcgcg
     2041 cttcggcttg actcataccg attcgttgag ccaatccaaa cggtgaaata ccgtaaatga
     2101 cgccgaaatt aatgtcttta gcctttcggc gcaagtcctt tgtcacttca ttggcctcaa
     2161 cgccgaacac ccttgcggcc gtactcgtgt gaatatcggc atcctcggaa aatgccgcta
     2221 ttaaattttc atctccggac atgtgcgcca taacacgaag ttcgatctgc gaataatccg
     2281 ccgatagtaa aacccaacct ttttcctgtg ctgtaaaggc gcgccgaatt tcacggccca
     2341 tgtcagtacg gattggaata ttttgaaaat tgggattagt tgatgataaa cgcccagtag
     2401 cggcgatcgt ttgattgaat gtcgtgtgaa tacgcccggt tcgaggatta cgaagcgggg
     2461 gaaggccgtc aatataagtt gacttaagtt tggtcagctg gcggtaattg agaatagcct
     2521 caacgatcgg tagcgtacga tattgttcta atattttcac atccgtggca tattgtttgg
     2581 tcttacccgt tagtttcggt ttttcgatgc cggccaattt atgaatttgc agtttttcaa
     2641 aaagtatttc accaagctgt tgaggcgaat tcatattgaa gtccgttccg gcttctttga
     2701 aaatgcgttg ttcttcctgt atgatttgta tggcaaaatc tttggacatc gtacttaaaa
     2761 tttccaaatc aagccatacg ccgtggcgtt ccattctctg aagcaccgga ataagcggca
     2821 tttcaatcgt attaaaaatt tttgcactat gtgtttcttt gagcaagggg gacatgacat
     2881 tttttaaacg tacgacgcag tccgcgtctt cattggcata agcgctgact ttttcgatgg
     2941 ggacatcccg catcgtaatc tgatttttcc cggtgccgat cagatccgtt gtggggattt
     3001 tttcaatccc aagataggac gcagctagag cgtcaatgtt gacctctttg tcggagtgca
     3061 aaagatgccc cgctatcata gtgtcaaaag cagttccttg tacttcgata ccataatgtg
     3121 atacgatgat gatatcatat ttggtatttt gcccccactt gggaatagtc gaatcttcca
     3181 atacgggttt caataaagta caaattgtgt cgacaggaac gtccccattg caaggtatgt
     3241 aaaaggcttc accctcggtc caactgaatg aaaagcccac taactcagca cgcatcggat
     3301 cggcatccgt cgtttctgtg tcccacgcga agccgcctga gttcgttaac ttggtgacaa
     3361 gttcttttat agccacttcg gtttggagcg tatgataatt acgagcgggt tccggcgtac
     3421 gttcgtgctg aagcggtgta tcctcgacga ataaatcaga ttcgtttttt aaaatttttt
     3481 taatcagaga cttgaattcg agttcggtaa gaaggcgagt gactttttga tgatccggcg
     3541 tccgaccacg cagatcatgg atatcttcgc gaagcggcac atccagtgca atcgttgcga
     3601 gcgacttgga aaggaaagca tcctctttat gttgttccag gttttcgcgc aactttcctt
     3661 tgatgctatc aatttgttcg tatatttttt cgatggagcc gtattgattg atcaatttgg
     3721 ccgcggtttt ttcgccgatg ccttttacgc cgggtatatt atcagcggca tcacccatga
     3781 ggcccagtat atcggtgact tgcgacggtt cgacgccaaa tttttcaaga acgccatcgc
     3841 gtccgatgat ttctttttcg ccgccttttt ttaagggatt atacattacg atattggggc
     3901 cgatcagctg catgaaatct ttatcacccg tcaccatgtg cgctacgata ccttgctgtg
     3961 cggcacgcac ggacaatgtt ccaatcacat catccgcttc gtagcccggg tactcgagaa
     4021 taggtacgcc catcgcttcg gtcagttctt tgagataatg aagctgcggc accatgtctt
     4081 ccggcatttt ttcacgcgtt gctttgtatt cgctatattg tttgtgtcga aacgtaggtt
     4141 cagcaagatc aaacgcaacg gcgatataat ccggtttttc ttcatctcgg attttgatca
     4201 aagcattgat aaaaccgaat accgcgctgg tattttcacc tttagcattt ttcaaaggat
     4261 tatgaataaa tgcaaaatat gcgcgatagg ccaaggccat accatctaat aagaataaac
     4321 gttcggacat aaaaagtatg attggttgat aaattaaaat tgcataatgt tataaatacg
     4381 acggcacatg cggcatcagc gtgtttttat gatcgttgta aaattgttga aatcggagaa
     4441 tatttacctc cgattcgtcc aataccgcaa aagatttgtc tgtcaaaggt tcatgatatc
     4501 cgcaaccaaa attaaacgct ttgcactcca gatgatccat taaaacaatg tctgagcggt
     4561 agccttcatg cacttcaaat ccgtattttt gcattagctg taggatttcc gcatcgccgt
     4621 attgatataa aacaacgccc gatccttttc gatcaaacga aaaaatccaa tggtattttt
     4681 tttccggtat aaacatttgt gccgtacttt cgccatgccg tgcatcggta cttaagagta
     4741 catcggcagt cactccgatg gaaggaagta attccgtaat gatatatacc ccaaggcgat
     4801 cgtccaatgt agtgcaatgc accgtcgaat gaccgtcggt ttcttcaaaa tcaaatcccg
     4861 catatgcctg aacacaatcc agatgggcta cggcgaggat cgaagaatta ttttcatgga
     4921 aataatgcgt gccaccgtga agccaagatt cttcgtcacg ttcgcaaatc agatgcagcg
     4981 attcgatatc ccaaggtaac tttaaactca tagcgtatcc cgtaatgcgg atgaaaaaac
     5041 gatactattg cgacgggtga catctaagtt ttgccgactg accgtggcaa gagttttcca
     5101 aaatgtatga accggcaata ggcagtacca tttaccgtcg ttataacgtt gatagagtac
     5161 cgtatctccg gcggacgtga tgacagaagc aaaaccatct tcctcgatga ttttttcagg
     5221 gtgtgcacga taggcctgga taatgttttc ccaccacgaa acattaccga tactg
//
LOCUS       DAOQNQ010000226         5222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_6496_length_5222_cov_4.675053, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000226 DAOQNQ010000000
VERSION     DAOQNQ010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6496_length_5222_cov_4.675053"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PK754_12145"
     CDS             <1..587
                     /locus_tag="PK754_12145"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNB10302.1"
                     /translation="SAKGLEFPLVVVAGLEEGLFPLENARNNARELEEERRLFYVSTT
                     RAQNELYLSFAKRRMRFNNIYQTDRSRFIEEVPAKLLDWQKISTYEPRLFEGSGKSWQ
                     SKNYSGSERVTAYEDGPSRVKKSLKDFSDEIVTSAAITETKYRIGQQVIHASFGTGTI
                     KNSEGQGDKQKLTILFGDGVERRLMVKYANLMVK"
     gene            610..4407
                     /locus_tag="PK754_12150"
     CDS             610..4407
                     /locus_tag="PK754_12150"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB10303.1"
                     /translation="MNIRNSSILLTIILSLILSTPAHTQVSSADTDFLYARKLYDDKL
                     YALAAQEFGKFIRNYPSDMRIPDARYYSGMAQFNEKNYEQARRDFQFLAIDYPKDKRA
                     PDAWAKIAECYAAMGDYPSAANALTSIATFYPTAPNAVTSIIEASEYFIKAGDLRSAK
                     DRLQKLIADRPDIPEANVSRFKLAMLLKNENDWPAASGALQTVIEKSKDQELVASAMF
                     EQAGIADRLGKSDEARNAYVRIVTRYSKTKINVYALFELGNILFRERNFIDARKNFEI
                     VISNILAPEPLKHAAQINLGDILFVNEKYTLALDLYKRVASEKNDSLNALEANFKSGL
                     ASEKLNLPVAAGDYYYTITEQYASVFQSSQFVKWATLKLAQISFQLKRYKEAGRYYED
                     YITKYPKSADLDRVLYDRGILLKDRLADYAEASRMFTRLISEFPNSEKIDDAYLALAQ
                     SLRMDNQISEALDALEKLKNNYPGTLLWNDALDERRRIEYFHQYGNNTTTQSIIYLIG
                     NLIEDKPKDEITFAYARLFFEQLKDYEGAAGLFKKTILSTKNRALTEEALFYLAQSYD
                     RISEMKPTQAGYADSAVGVYKQLAVGKYADVATVRVIESTLKTISNYTDRQRKAKEMY
                     AGLLERFPGSTLKPNMLKGLGDAMMALGELKPVIVPQVPSVTKSKDKSPVTAKPDSLK
                     PLSSAMQCYEAIIKDFPDNPVAEDAFYSMAEAYWFSKQPDLYYQSLIRYAAQYPRGRY
                     IVKTKMLMARYKEEKSDYALALNNLNEIITHYFYTPYADSAAQSVGHNYLLSGQYTQA
                     IKAYLNARTQGEFALEQMDIIAAQQKIHHPLDYRLAYCYDKSGNFTKAVEYYEAYLFP
                     DNKGEYALHALTALAQMYEKKQDFQIALPYYQRIAQLYPETEAGFKALARASEMYFNS
                     EQYDSAKVAFTKLALYTKDKMQQIVFESRTIVCAYRLGQIVETGKLEKDFNKKYESDK
                     NLKLLLLNYNAEFLFELGRYYQYNKTRADYAQAVKTYTRVIEDYKTAAIMPEVLFEMG
                     VIKFNEGKSKEGFEYFQQIPQKWPDSDILPRVHLRTAFEAFRLENVQLAVEASKLALQ
                     NPRIKLADAKLGTNFLIKVYKAAGYYENGLMLMQQYLERFPDDDPATIWAMRIDIGAM
                     HKNLKSFDRALEYFKDLIKTASGEDEAEIQFNIAETYWAMNNFEQALLEYLRIPYLTL
                     GKKFDWSSAAKSQAAECYVKLSKYDEAIRMYNDIIRVNGPQSEYSAYAKQRIEQIQGL
                     KKN"
     gene            4407..5219
                     /locus_tag="PK754_12155"
     CDS             4407..5219
                     /locus_tag="PK754_12155"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNB10304.1"
                     /translation="MMIEIPRQARSVAIGAALQAARIITDYYGRVFTIDAKLHNDFVT
                     EVDRKSEEVIIQHILRAFPDHAILAEETGKHRNESHFRWIIDPLDGTTNFIHGMPMFA
                     VSIALEVHGQLAVGVVYEPLRKEMFVAEHGKGAFLNDRKIRVSATTEHSRCLLATGFP
                     FKTFDFIDDYLNIFKTFMTQTAGIRRPGSAALDLCYLACGRFDGFWELNLNPWDIAAG
                     TLIIQEAGGRITGFDGQNNFLYKGHVIGSNGILHDWMLSNVQKSLGEKISLL"
BASE COUNT         1555 a         1113 c         1161 g         1393 t
ORIGIN      
        1 acagtgccaa aggattagaa ttcccattag tcgtggtcgc gggtcttgaa gaaggtttgt
       61 ttcctcttga gaatgcacgc aataatgcgc gcgaactgga agaagaacga cggttgtttt
      121 atgtaagcac cacacgtgcg cagaatgaat tatatttatc attcgccaaa cggcgtatgc
      181 ggtttaataa catttatcaa accgatcgtt ctcgttttat tgaagaagtg ccggctaaat
      241 tgcttgattg gcagaaaatt tcaacctatg agccgcggct attcgaaggg tcggggaaat
      301 cctggcaatc caaaaactat tccggttccg aacgcgttac agcgtatgaa gacggtcctt
      361 cacgtgtcaa aaaatcgttg aaagattttt cagatgaaat tgtcacatcg gctgcgatca
      421 ctgaaactaa atatcgtatc ggtcagcaag ttatccatgc ttcgtttgga accggcacca
      481 taaaaaacag cgaaggccaa ggcgacaaac aaaaactcac gattcttttt ggtgatggag
      541 tagagcgccg tctgatggta aaatacgcca acctaatggt caaatagtgt acatacgaaa
      601 gactatgtta tgaacatccg aaattcatct atcctgctta cgatcatact ttcgttgatc
      661 ctttctacgc cggcacacac acaagtgtcg agcgccgata ccgatttttt atatgcccgt
      721 aagctgtatg atgataagct ctatgcgctc gcagcgcagg aattcggaaa atttatacga
      781 aattatccct ccgatatgcg cattcctgat gcccggtatt attcgggtat ggcgcagttt
      841 aatgaaaaaa attatgaaca ggcgcgacgt gactttcaat ttcttgcgat tgattatccc
      901 aaagataaac gtgcgccgga tgcgtgggcc aagatagcag aatgttatgc ggcaatgggg
      961 gattatccct ctgcagccaa tgcgcttacg agtattgcaa cattttatcc caccgcaccc
     1021 aatgccgtaa cgtccattat cgaggccagt gaatatttta tcaaagccgg cgatctgcgc
     1081 agcgctaaag atcgtttgca aaaactcatc gccgatcgtc ccgatattcc ggaagctaat
     1141 gtcagccgct tcaaactggc gatgcttctc aaaaatgaaa acgattggcc ggccgcttcc
     1201 ggtgcacttc aaacggtcat tgaaaaatca aaagatcagg aattggtggc ttcggccatg
     1261 tttgaacagg cgggaatcgc ggatcgcctt ggtaaatccg atgaagcgcg aaatgcttat
     1321 gtccgaatcg taacacgata cagtaagacc aagatcaacg tatatgcctt gttcgaatta
     1381 ggcaatattc tattcagaga gcgcaatttt attgatgcgc gtaaaaactt tgaaatcgtc
     1441 atttccaata tacttgctcc ggagccgctc aaacatgccg cacagatcaa tctcggcgat
     1501 atacttttcg tcaatgagaa atatactctg gcgcttgatc tgtataaacg cgttgcatcc
     1561 gaaaaaaacg atagtctaaa tgcactagag gccaatttta aatccggttt ggcgtcggaa
     1621 aaactgaacc tcccggtcgc cgccggtgat tattattata ctatcaccga acaatatgcc
     1681 tcagtttttc aatcatccca atttgtcaaa tgggcgacac tcaagttagc gcaaatttca
     1741 tttcaactaa aacgctataa agaagcgggt cgttattatg aagattatat cactaaatac
     1801 cctaaatctg ccgatttaga tcgcgtttta tatgatcgcg gtatattgct caaagaccgg
     1861 ttagctgatt atgcggaagc atcgcgcatg tttacgcgcc tgatcagcga atttccgaac
     1921 agtgaaaaaa tagatgacgc gtaccttgct ttagcgcagt cgttgcgtat ggataatcag
     1981 atatctgaag ctcttgatgc attagagaaa cttaaaaaca attatcccgg aacattactc
     2041 tggaatgatg ccctggatga gcgccgacgt atcgaatatt ttcatcaata cggtaataat
     2101 acgactacgc aaagtataat ttatctgatc ggtaacctta tcgaagataa gccgaaagac
     2161 gaaatcacat ttgcttatgc gcgtttattc tttgaacaac tcaaagatta tgagggtgcg
     2221 gcaggattat tcaaaaaaac catcctctcg actaaaaacc gcgctttgac ggaagaagct
     2281 ttattttatc tcgctcaatc ctatgaccgg atatcagaaa tgaagccaac acaggccggt
     2341 tatgcggaca gcgctgtcgg tgtgtacaaa caattagccg taggcaaata tgccgatgtc
     2401 gccaccgtgc gtgtcatcga aagcacactg aaaacgatta gtaattatac cgatcgtcag
     2461 agaaaagcta aagagatgta cgccggattg ctggaacgat ttcctggttc aacgctaaag
     2521 ccgaacatgc tcaaaggatt gggtgatgcg atgatggcgc tcggtgaatt aaagcctgtt
     2581 attgtcccac aggttccttc cgttaccaaa agtaaagata aatcacccgt tactgcaaaa
     2641 cccgattcac tgaaaccatt gagttcggcg atgcaatgtt atgaagcgat tatcaaagat
     2701 tttccggaca accctgtggc cgaagatgcg ttttatagta tggccgaagc gtattggttt
     2761 tccaaacaac cggatttgta ttatcagtct ttgatccggt atgcggcaca atatccacgc
     2821 gggcgctata tcgtcaaaac aaaaatgctt atggcgcgct ataaagagga aaagtccgat
     2881 tatgcgcttg ctttgaataa tctcaatgag atcatcacgc attattttta tacaccgtat
     2941 gccgatagtg ctgctcaatc ggtaggacat aactatcttc tgtcaggcca atatacccaa
     3001 gcgatcaaag cgtatcttaa tgcgcgcaca cagggcgagt ttgcattgga gcagatggat
     3061 attattgccg cgcaacaaaa aatacatcat ccgcttgatt accggttggc ttattgctat
     3121 gacaaatccg gtaattttac caaagcggtg gagtactacg aagcgtatct ttttccggat
     3181 aataaaggcg aatacgcgtt gcatgccctg acggcgcttg cgcagatgta tgagaagaag
     3241 caagattttc agattgcact gccttattat caacgtatcg cgcagttata tccggaaacc
     3301 gaagccggat tcaaagcgct ggcacgggcg tcggagatgt acttcaattc cgagcaatac
     3361 gattcggcca aagtagcgtt tacgaagcta gcgttatata ccaaagataa aatgcagcaa
     3421 attgtttttg aatcacgcac gatcgtatgc gcataccgtc tgggccaaat cgtcgaaacc
     3481 ggaaagttag agaaagactt taataaaaaa tacgaatcgg ataaaaacct taaactctta
     3541 ttgctgaatt ataacgcaga atttttattc gaattaggtc gttattatca atacaataaa
     3601 acacgcgccg attacgctca agctgtaaaa acatatacgc gtgtgatcga agattataaa
     3661 acagcggcca tcatgcctga agttttgttt gaaatgggtg tgatcaagtt taatgagggc
     3721 aagagcaaag aaggctttga atactttcag caaattccgc aaaaatggcc ggatagtgac
     3781 atacttccgc gtgtccatct gagaacagct tttgaagctt ttcgtctaga aaatgttcaa
     3841 ctcgccgttg aggcctctaa gttggcgctt caaaatccac gtatcaaatt agccgatgcc
     3901 aaattgggga ctaatttttt gatcaaagtg tataaagccg cgggatatta tgaaaacggg
     3961 ttgatgctta tgcagcaata tctcgaacgt tttccggacg acgatccggc aacgatttgg
     4021 gccatgcgta ttgacatcgg cgccatgcac aaaaacttga agtcctttga tcgtgcactt
     4081 gaatatttta aagatctcat taaaacggct tccggtgaag acgaagcgga aattcaattt
     4141 aatatcgcag aaacgtattg ggcgatgaat aatttcgaac aggccctgtt ggaatactta
     4201 cgcatcccat atttgacatt ggggaaaaaa ttcgattggt ccagtgccgc taaaagccaa
     4261 gcggcggaat gttacgtgaa attgtccaaa tacgatgaag ccatacgaat gtataacgat
     4321 attattcgtg tcaatggtcc gcaaagcgaa tacagcgcct atgccaagca gcgaatcgaa
     4381 caaatacaag gtctgaaaaa gaactgatga tgatcgaaat tccacgacag gcgcgcagcg
     4441 tcgctattgg agcggcatta caggcggcac gtattattac cgactattac gggcgcgttt
     4501 ttacgattga tgctaagttg cataacgatt ttgttaccga agtggatcgt aaatcggaag
     4561 aagttatcat acagcacatt ttacgtgctt ttccggatca tgctatttta gcggaagaaa
     4621 ccggtaaaca tcgtaacgaa agccattttc gatggattat agacccattg gatggtacca
     4681 ccaattttat tcacggaatg cctatgttcg ccgtaagcat tgcgttggaa gtgcacggtc
     4741 agttagccgt gggcgttgta tatgaaccgt tgcgcaaaga aatgtttgtc gccgaacacg
     4801 gtaaaggcgc ctttttaaat gaccgcaaga ttcgggtgtc tgccactacg gaacatagtc
     4861 gttgcctttt agctaccgga tttcctttca agacgtttga ttttattgac gattatctca
     4921 atatatttaa aactttcatg acgcaaacgg cgggcattcg aagacccggc tccgccgcat
     4981 tggatttatg ttatcttgcc tgcggccgtt tcgacggatt ttgggaactc aacctcaatc
     5041 cttgggatat cgcagccggt acattgatca tacaagaagc cggcggacgt attaccggtt
     5101 ttgacggaca aaataatttt ttgtacaaag gtcatgtaat cggttccaat ggtattttac
     5161 atgactggat gctttcgaat gttcagaaat cactcggcga gaaaattagt ttactctaaa
     5221 ta
//
LOCUS       DAOQNQ010000227         5212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_6512_length_5212_cov_5.963157, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000227 DAOQNQ010000000
VERSION     DAOQNQ010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6512_length_5212_cov_5.963157"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PK754_12160"
     CDS             complement(<1..215)
                     /locus_tag="PK754_12160"
                     /inference="COORDINATES: protein motif:HMM:NF015441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HNB10305.1"
                     /translation="MVQISDLRIYPIKSCKGFSVPSVTLDAYGFTDDRRFMIVDTSGR
                     FVTAREVNPLLWIEPSIESGILYMRAPG"
     gene            complement(235..984)
                     /locus_tag="PK754_12165"
     CDS             complement(235..984)
                     /locus_tag="PK754_12165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB10306.1"
                     /translation="MDIIFKDHFSGHAEAYATFRPHYPEPFYAWLISLTARQENAWDC
                     GTGNGQAAIRLAESFDTVTATDASAQQIRQAQPHPKVHYYVEPAEQSHLETGSVDIIT
                     AAASIHWFDMHAFYGEVKRVARPDALLAVWAYDRVKISNEIDILVDRLYYDIAGPFWP
                     KERRWVEEQYKTIPFPFEEISAPSFAINHTLTVDDLIGYLRTWSSVQGYLKVHGKDPV
                     DWITGELHNLWGSEKRPVEWPMFMRIGRIHK"
     gene            complement(975..1358)
                     /locus_tag="PK754_12170"
     CDS             complement(975..1358)
                     /locus_tag="PK754_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNB10307.1"
                     /translation="MKTKLRIQNGPAALSVVRLASDAPWPLWLQSKDGFFSVTRTDEE
                     LSIVCKQILVPPDARAEHGWRAFKIVGPIPFEMTGVLASVIAPLGEAGISIFAVSTFD
                     TDYILVKEENWSPARAVLDDVVEWI"
     gene            complement(1355..2500)
                     /locus_tag="PK754_12175"
     CDS             complement(1355..2500)
                     /locus_tag="PK754_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB10308.1"
                     /translation="MQKKLSDDSLCIHAGTKRSGEFPGVNTPIYTSTAFEYKTAGPMV
                     YPRYYNTPNQQAVIEKIAALEKAEAGVLFSSGMAAISTTMLTLARKGDHILLQYDIYG
                     GTYHFAATQLEPLGIEYSFVRSLDPAEYEKAIRPNTKLIYIETPNNPLLKIIDIEAIA
                     AIGKKHGVTTMIDNTFASSVNQNPIALGIDIVMHSGTKYLGGHSDICCGVVVTREALA
                     DAIRKKANMFGGSLNAETCYLLERSIKTLSVRVDRHNHNAMTLAEALQNEKRIKRVYY
                     PGLASHDGHAVAKRQMRGFGGMLSFDIDTAVISPDQFLEQLRLIKPALSLGGVESTLC
                     MPSKTSHLRLSAEEKKQAGITDALMRLSVGIENATDILDDIREALRS"
     gene            complement(2516..2947)
                     /locus_tag="PK754_12180"
     CDS             complement(2516..2947)
                     /locus_tag="PK754_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNB10309.1"
                     /translation="MKSFYFPMMLTVASNVIYHIAQKSIPAHLNPIIPTIIAYAVALT
                     TSLCLYPFFSDTHLTQAGWRDVNWATLAVGMAIVGIEIGFLLAYRAGWNISLGAVTSN
                     VTVTLLLLPIGILFFKEHLSWEKITGIVLCMGGLWLIFKPS"
     gene            complement(2971..3483)
                     /locus_tag="PK754_12185"
     CDS             complement(2971..3483)
                     /locus_tag="PK754_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB10310.1"
                     /translation="MRPTKDEYPSFFETYVSKVPDGDIVQQMRQGVENTVKLISPLSD
                     DLLNYRYAEGKWSIKEVLMHLSDAERIFSYRALRFARKDKTDLPGFDENIYVPASKAT
                     QRTAESILNEYQTVRAASITLFESLDDEALQERGTANGRTTSVRAMAYILVGHELHHV
                     GIIKERYLKK"
     gene            complement(3533..4009)
                     /locus_tag="PK754_12190"
     CDS             complement(3533..4009)
                     /locus_tag="PK754_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HNB10311.1"
                     /translation="MMESKHWPEVAAIYQAGIETGNATLETSVPSWEAWDKAHIPQCR
                     FVVIDNNIIAGWAALTPVSGRCVYAGVAEVSVYIHPAYKGKGIGKKLLDQLALSSEAQ
                     GLWTLQAGILRENTASLKLHAACGFREIGYREKLGQYQGVWRDVVLMERRSKNVGQ"
     gene            complement(4044..4613)
                     /locus_tag="PK754_12195"
     CDS             complement(4044..4613)
                     /locus_tag="PK754_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10312.1"
                     /translation="MLRMALTVSCVLILIGCDEDKTSSEKEIFHRSLNDRAVKYLSPQ
                     TVDLDSNGAEDYSFTTSLMQEDGKTVLQFVINGTTSNAVLYNTSSAEIPALAQNTLIS
                     SSPASPKQWDAFENDLCSILYTSATDSVWRGNWSKANHQFVGVRFTVSDQHHYGWISM
                     SADTTNGQLILHACAFNTTAGGSIKAGEK"
     gene            complement(4722..5180)
                     /locus_tag="PK754_12200"
     CDS             complement(4722..5180)
                     /locus_tag="PK754_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB10313.1"
                     /translation="MNIIRCEERHIDKLVSLFDAYRMFYHQSSDESAAKIFLSARIRN
                     GESVVFIALDTNENAVGFTQLYPLFSSVSMQPVWLLNDLYVSQTARRSGVGEALMEKA
                     RRFAVETGAKGIELETGVENVQAQALYEKLGYQRNTDTYRYFLPVTAALK"
BASE COUNT         1354 a         1306 c         1125 g         1427 t
ORIGIN      
        1 cccggcgccc gcatatacaa aatgccgctt tcgatcgagg gttcgatcca taacaatggg
       61 ttcacctcac gcgccgtcac aaaacggccg gatgtatcca cgatcataaa acgccgatca
      121 tcggtaaatc cgtacgcatc caacgtaact gacggaacgg aaaacccttt gcaggatttg
      181 atcggataaa tgcgaagatc gctgatttgt accatttttt ttttaaagtt aatgttattt
      241 gtgaatacgc ccgatgcgca taaacatcgg ccattcgacc gggcgtttct ccgaacccca
      301 aagattgtgc agttcaccgg ttatccaatc aaccggatct ttgccgtgta cttttaaata
      361 accttgcacg ctggaccatg tacgcaagta gccgatcaaa tcatctacag tcagagtatg
      421 gttgatagca aaggaaggcg ctgaaatttc ctcaaatgga aacggtatcg ttttatattg
      481 ctcctcaacc cagcgtcgtt ctttgggcca aaatggcccg gctatatcgt aatacaaacg
      541 atctacgagg atatctattt cgttagaaat ctttacacga tcatacgccc atactgcgag
      601 aagagcgtcc ggccgagcta cacgtttgac ttcgccgtaa aacgcatgca tgtcaaacca
      661 atgaatggat gcggccgctg tgatgatatc cacactacct gtttccaaat gactttgttc
      721 tgcgggttct acataataat gaactttcgg atgcggttgc gcttggcgaa tctgctgtgc
      781 gctggcatcg gtcgccgtaa cagtatcaaa agattctgct aacctgattg ccgcctgccc
      841 attaccggtg ccgcaatccc acgcgttttc ttgccgcgcc gtaagactta tcagccacgc
      901 ataaaagggt tccggataat gcgggcgaaa cgtcgcatag gcctccgcat gaccagaaaa
      961 atgatcttta aatattatat ccattctacc acgtcgtcga gcactgcacg tgcagggctc
     1021 caattttctt ctttgactaa aatataatcc gtatcgaacg tcgagacggc aaaaatactg
     1081 atcccggctt caccaagcgg tgcgatcacc gaagccaata cgcccgtcat ttcaaaaggt
     1141 atcggtccca cgattttaaa agcacgccaa ccgtgttcag ctcgggcatc cggcgggaca
     1201 aggatttgtt tacacacgat actcaattcc tcatctgttc gtgtaaccga aaaaaaacca
     1261 tctttcgatt ggagccagag aggccacggc gcatccgatg ctaaacgaac aaccgataaa
     1321 gcggccggcc cgttttgtat tcggagtttg gttttcatga acgcaacgcc tctcgaatat
     1381 catcaagtat atccgtggca ttttcgatac cgacggacaa tcgcatcagc gcatccgtaa
     1441 tacccgcttg ttttttttcc tcggccgaaa ggcgcagatg tgaagtttta gaaggcatac
     1501 acagtgtgga ttctacaccg ccaagactca gcgccggctt gatcaatctc agttgttcta
     1561 aaaattgatc cggtgaaata accgcggtat caatatcaaa ggataacatc ccgccaaaac
     1621 ctcgcatctg acgcttcgca acggcatgac cgtcgtgcga tgccagcccg ggataataaa
     1681 cgcgtttgat acgtttctca ttttgcaatg cttcagccag cgtcatcgca ttatgattat
     1741 gacggtcaac ccgaaccgaa agcgttttga tgctgcgctc cagcagataa cacgtttcgg
     1801 cattgaggct accgccgaac atgttggctt tttttcgaat tgcatccgct aacgcttcgc
     1861 gggtaacgac cacaccgcaa caaatatcgc tatgcccgcc gagatacttg gtaccgctgt
     1921 gcattacgat gtctattccg agtgcgatag gattctgatt gacggacgac gcaaacgtat
     1981 tgtcaatcat cgtcgtgacg ccgtgctttt taccgatggc cgcgatcgcc tctatatcta
     2041 tgattttgag cagcggattg ttgggcgttt cgatgtagat caatttggtg ttgggccgaa
     2101 ttgccttttc atactctgca ggatccagcg atcggacaaa cgaatattca ataccgagcg
     2161 gttccagttg tgtcgctgcg aaatggtacg taccaccgta aatatcgtat tgcagtaaaa
     2221 tatgatcgcc tttgcgggct aatgtgagca tcgtcgtgct gatcgccgcc ataccggaac
     2281 taaacaaaac accggcttcg gctttttcca atgcggcaat tttttcgatg accgcctgtt
     2341 ggttcggtgt attgtaatat cgcggataca ccatcggccc ggcggttttg tattcaaatg
     2401 ctgtagacgt ataaatcggt gtattgacac cgggaaattc accgctgcgt ttggtaccgg
     2461 catggataca caaggagtca tccgataatt ttttctgcat cacaatttcc tttttttaag
     2521 aaggtttgaa aatcaaccac aagccgccca tacataaaac aataccggtt attttttccc
     2581 atgacaaatg ttctttgaaa aacagaatgc ctatgggcaa aagtaaaagt gtcacagtca
     2641 cattggatgt cacggcgcca agactgatat tccacccggc acggtaagcc agtaaaaaac
     2701 caatttctat gccgacgatc gccataccga cagccaacgt ggcccagttg acatcccgcc
     2761 aacccgcttg cgttagatgg gtatcgctaa aaaacggata caaacataac gaagtcgtca
     2821 acgctaccgc ataagcgatg atcgtcggaa tgatcggatt gagatgcgcc ggtatggatt
     2881 tttgcgcgat gtggtagatc acgttggatg ccaccgtcaa catcatcgga aaataaaaag
     2941 atttcataga tctctcggtt aatagccgat ttattttttt agatagcgct ctttgatgat
     3001 gcccacatgg tgcaattcat gtccgactaa aatataagcc atcgcacgta cgctggttgt
     3061 gcgcccgttg gccgtgccgc gctcctgcaa tgcttcgtcg tctaaacttt caaatagcgt
     3121 gatcgaagcg gcccgcacgg tttggtattc gttcaaaata ctctctgcgg tacgctgcgt
     3181 tgctttggac gcgggtacgt atatgttttc atcaaacccc ggcaaatcgg ttttgtcttt
     3241 acgagcaaaa cgaagcgcac gatacgaaaa aatccgttcg gcatcggata agtgcatcaa
     3301 aacttctttg atactccatt ttccttcggc ataacgatag ttcaacagat catcgctcaa
     3361 tgggctaatg agttttacgg tattctctac accttgccgc atttgttgta caatatcgcc
     3421 gtccggtact ttgcttacgt acgtttcaaa aaacgagggg tattcatctt tcgttggacg
     3481 catatgcgct cctgtttgtt atggcttatt attgatgacg tctttcaata acttactggc
     3541 cgacgttttt actgcggcgt tccattaata ccacgtcgcg ccaaacgcct tgatactgac
     3601 ctaatttctc tcgatagcct atttcacgaa atccgcaggc ggcgtgtaat ttcaaactgg
     3661 ctgtattctc gcgtaaaatt cccgcctgta aagtccacaa tccttgcgct tccgatgaaa
     3721 gcgccaattg atcaagtagt ttttttccaa tgccttttcc cttataagcc ggatgaatat
     3781 atacactcac ctcggccacg ccggcatata cacaacggcc ggataccggc gtcaatgcag
     3841 cccatccggc gataatatta ttatctatca ccacaaaacg gcattgcggt atatgtgctt
     3901 tatcccatgc ctcccaactc ggtacggatg tttcaagcgt cgcatttccc gtctcaatgc
     3961 cggcctgata tatggcggcc acttccggcc aatgcttaga ctccatcatt tgcaatgcgt
     4021 atgtatttgt tgtcgcgttc actttatttt tctccggctt tgatggatcc accggccgtc
     4081 gtattaaaag cgcatgcatg caaaatcaat tgcccattag ttgtatcggc gctcatgctg
     4141 atccacccgt aatggtgttg atcgctgacg gtaaaacgta cgccaacaaa ttgatgattc
     4201 gctttagacc aattgccccg ccagacggaa tcggtagctg atgtataaag gatcgaacac
     4261 aaatcatttt cgaacgcatc ccattgttta gggcttgcgg gcgacgatga aataagcgtg
     4321 ttctgagcaa gagcgggtat ctccgctgat gaagtattgt acaacaccgc attggaagtg
     4381 gtaccgttga ttacaaactg caacacggtt tttccgtctt cctgcataag cgatgtggtg
     4441 aaggaataat cctccgcacc gttgctatcc agatccaccg tctgcggtga cagatatttg
     4501 acggcgcgat cattgagcga acgatgaaaa atttctttct ctgacgaagt tttatcttcg
     4561 tcgcatccga tcaatatcaa aacacacgat acggttagag ccattcgtaa catacgtttc
     4621 cccgatttag ttacttagaa atagtgaatt gtaaaacata acttttttaa tctgtacgta
     4681 tatcatatct gcatattttc ttagtacgcg ttttttgaac tttatttcaa cgctgctgtt
     4741 acgggtaaaa aatagcgata cgtatccgtg ttgcgctgat aacctaattt ttcatacaac
     4801 gcctgcgctt gaacattttc aacacctgtt tccagttcaa tgcctttagc gcctgtttcg
     4861 acggcaaagc gacgtgcttt ttccatcaaa gcctcaccga cacccgatcg gcgcgccgtt
     4921 tggctcacgt ataaatcatt aagtaaccaa accggttgca tggacaccga tgaaaaaaga
     4981 ggatacaact gcgtaaaacc cacggcgttt tcattcgtat ccagggctat aaaaacaacg
     5041 gactccccgt tacgaatccg ggccgataaa aaaattttgg ccgccgattc atcggacgat
     5101 tgatgataaa acatccgata tgcatcaaaa agtgatacca gtttgtctat atgccgttct
     5161 tcgcagcgta tgatattcat ccgcgtttcc ttttattctt cgatgttttt tt
//
LOCUS       DAOQNQ010000228         5192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_6569_length_5192_cov_3.735449, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000228 DAOQNQ010000000
VERSION     DAOQNQ010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6569_length_5192_cov_3.735449"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1508
                     /locus_tag="PK754_12205"
     CDS             <1..1508
                     /locus_tag="PK754_12205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB10314.1"
                     /translation="SVTLRSIGDGVITTDVQGRIVLINRVAEVLTGWTQEEAQDKPIE
                     DVFHIINYKTKVRIAEPVRRIIQSGGIFELEQNTALIARDGTERLIADSGAPIRDKES
                     TIIGVVLVFRDITEKQKIEEERLKSIKLESVGLLAGGIAHDFNNILTAILGNISLARM
                     EHQLHHYDKIDDILSRSEKAGFRAKELTQQLLTFAKGGSPVRKTLTISELIREAADFM
                     MRGSNVLCEYDMPEDLWPAEIDEGQISQVIQNLVINAVHAMPDGGVIRIKTENVFLDE
                     NAVMHLAAGKYIRMDIADHGVGIAPEYLSKIFDPYFTTKQRGSGLGLATCYSIVKNHQ
                     GAISVVSQPGAGSVFTVYLPASEKQVQQLSAPQKELGMGSGKILVMDDEEDIREITTR
                     ILRKFGYQTDSVTDGTDAIRAYTAARDAGVPYDVVIMDLTIPGGMGGKETIKKLLEID
                     PNVRAVVSSGYSNDPIMADHKSYGFKGYIIKPFQVDAFRQLIHDVINDKNE"
     gene            1563..1635
                     /locus_tag="PK754_12210"
     tRNA            1563..1635
                     /locus_tag="PK754_12210"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1596..1598,aa:Lys,seq:ctt)
     gene            2287..3450
                     /locus_tag="PK754_12215"
     CDS             2287..3450
                     /locus_tag="PK754_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10315.1"
                     /translation="MRNDSVGFANSFGLIGAEQARVVTATPDGGLFIGGRANSLRGDY
                     DLYLIRLKNDSKVMWAKTLGGGNFDELTDAVVSKNGDIYVIGSISRSATDINAFIGKL
                     SPQGNLIWTYEMEGISNTSGVALAMSNEGSLIGAVFEKTNSEDDGRAVMIKVSDEGNL
                     IWQKTIISLTPAEPKDILISPNDDIYIGGQTIQNTPYSSYSDALLIKANSLGDTVWTK
                     RYHSLTDPATGLIPNRDIIRALVFENSGHIVIAASSLLSFPHYGHTDFWFVRVDTDGN
                     IKLQTKYREQFRDDVFPILLPHALSRLGNGDYVISGRFYDRFVGPRYQMWVARFTSQG
                     NMIWSKSFGDVLDDEGLGVEVIGNSIYSTGYTMTDSLSTDIYLLRLDLNGEIR"
     gene            complement(3766..4755)
                     /locus_tag="PK754_12220"
     CDS             complement(3766..4755)
                     /locus_tag="PK754_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNB10316.1"
                     /translation="MPANLSTSYMGFTLKNPIVPSASPLSKHLDNIRKMEDAGAAAVV
                     LFSLFEEQIYHDAMELVQEASKISEDFRNSFDYLRELNQSHYNPDAYLEHIRKAKEIV
                     KIPIIASLNGSSDNGWLQYAKSIEAAGADGLELNLYHIAADPKKNSAQIEDQFIHVVR
                     SVKNLKIPVSVKLSPHITGLANFVSRLDETRVDGVVLFNRFYQPDFNLNSLKVVPNIN
                     LSDSTDLRLALRWTAILYGQVHAPIAATSGIHSHEDAIKMIMAGASVTMVCSALLKNG
                     ISHITELLRDIELWMDIHECQALSHIRGSMSQKSKDDPSAMERAQYIKALAQY"
BASE COUNT         1475 a          999 c         1238 g         1480 t
ORIGIN      
        1 tatctgtgac actgcgttcg atcggtgacg gcgtgatcac gacggatgtg caagggcgga
       61 tcgttttgat caatcgtgtt gccgaagtat tgaccggatg gacacaagaa gaagcgcaag
      121 acaagccgat cgaggatgtt tttcatatca tcaattacaa aaccaaagtg cgaattgcgg
      181 agccggtgcg ccggatcatt caaagcggcg gcattttcga gctggaacaa aatacggcgt
      241 tgatcgctcg tgatggcaca gagcgattga tagccgacag cggtgccccc attcgcgata
      301 aagaaagtac gatcatcggt gtggtgcttg tgtttcgaga tatcacagag aaacaaaaaa
      361 tcgaagaaga acgtctgaaa tctatcaaac tggaatcggt cggattactt gccggtggta
      421 ttgcgcacga ctttaataat attttgacag cgatattggg taatatttcg ctggcgcgta
      481 tggaacatca gctgcatcac tatgataaaa ttgatgatat attatcccgt tctgaaaaag
      541 ccggatttcg agccaaagaa ctgacgcaac aattactcac gtttgccaaa ggcggatcac
      601 cggtcagaaa aacgctgacc atcagtgaat tgatccgtga agcggcggac tttatgatgc
      661 gcggatccaa cgtgttgtgt gaatatgaca tgccggaaga tttatggcct gccgaaatag
      721 atgaagggca gatcagtcag gtgatccaga atctggtgat caacgcggta catgcaatgc
      781 ctgacggcgg agtcatacgt ataaaaacag aaaatgtatt tttagatgaa aacgcggtca
      841 tgcatcttgc ggcgggaaag tatatccgca tggatattgc agatcacggt gtcggcattg
      901 cgccggaata tttatctaaa atttttgacc cttattttac aaccaaacaa cgcggtagcg
      961 gcttgggttt ggctacttgt tattccatcg taaaaaacca tcagggtgcg atatccgtcg
     1021 tatcgcaacc gggcgccggt tctgttttta cggtgtattt gccggcttcg gaaaaacaag
     1081 tgcaacaact atcggccccg caaaaggaac ttggaatggg cagcggaaaa atacttgtca
     1141 tggatgacga agaagacatc cgggaaataa ccacgcgtat tctgcgtaag tttggttatc
     1201 agacggattc cgtcactgat ggcacagatg ccatacgcgc gtatacggct gcaagagatg
     1261 ccggagtgcc gtatgatgtc gtgatcatgg atctcacgat tcccggaggt atgggcggaa
     1321 aagaaacaat aaaaaagctt ttagaaattg atccgaacgt tcgagccgtc gtctcaagcg
     1381 gctattccaa cgatccgatc atggctgatc acaaatcgta tgggtttaaa ggttacatca
     1441 tcaaaccgtt tcaggtggat gcatttcgtc aactgataca cgacgtgatc aatgataaaa
     1501 atgaataaaa agagcttgcg tcgttagtgt gccgcgacta tattgcgtcc gattttttaa
     1561 atgcgtccat agctcaattg gcagagcaaa tgactcttaa tcattaggtt caaggttcga
     1621 ttccttgtgg acgcacaaaa acctgagttg tacaaactca ggttttttat ttagcgggat
     1681 ttattattga gaataatgta attgttaact cagcgcgata tacattcttt tcgttatcgt
     1741 agtgggcccg tagttttcaa tcattaggct tcccgctaca gcgggattcg attcctgtgc
     1801 atgcaaaaaa ttctgagtta gaccgactca ggttttttta ttcgtgggat ttattttgag
     1861 aataatgtaa ttgttaactc agcgcgatat acattctttt cgttatcgta gtgggcccgt
     1921 agttttcaat cattaggctt cccgctacag cggaattcga ttcctgtgca tgcaaaaaat
     1981 tctgagttag accgactcag gtttttaata tttgattcat aggaatcaga aatctctgca
     2041 tcgttgtaaa aatttattta atgagattgt atctaacaat ggaaccggca ggttagataa
     2101 ccccatgcat gttaataaat aattctaatt taaattattt gatattacgt aatataaaac
     2161 ataatattag ctataaaaac ataaaatagt gtattttatc agtaagtaat gtatcggatg
     2221 aaactgcaat tgtattgcct tctatgtttt gcgatcatct tgttttcgtc ttgcgaatct
     2281 tattctatgc gcaatgattc tgtaggattt gctaattctt ttgggcttat tggcgctgaa
     2341 caggcgagag tagtaacggc cacaccggat ggcgggcttt tcataggagg gcgggctaat
     2401 agtttgcggg gcgactatga tttatatctg atacgcctca aaaatgattc aaaagtaatg
     2461 tgggctaaaa cattgggggg tggcaatttc gatgaactaa cggatgcggt cgtgagtaaa
     2521 aacggagata tctatgtcat aggatctatc agcagatcgg caacggatat caacgccttc
     2581 attggaaaat tatcaccgca aggtaacctc atttggactt acgaaatgga aggtattagc
     2641 aatacgtccg gagtagcatt ggctatgagc aatgagggtt ctctgatagg cgcggtattc
     2701 gaaaaaacaa attcagaaga tgatggccgt gcagtaatga ttaaagtcag tgatgaaggg
     2761 aatttgatct ggcagaaaac aattatttcg ctcacacccg ctgaacccaa ggatattctt
     2821 atatctccga atgatgatat ttatattgga ggccagacaa tacaaaatac cccatatagc
     2881 agctatagcg atgcattatt aattaaagca aatagtctag gtgatacggt atggacaaaa
     2941 agataccata gtttgaccga cccagctacg ggcctaatcc caaatcgtga catcattcgt
     3001 gcactggttt ttgaaaattc cggtcacatt gtcatagcag ccagttcatt actttcgttc
     3061 cctcactacg gccataccga tttttggttt gtacgcgttg atacggacgg taatattaag
     3121 cttcaaacca agtacagaga gcagtttcgc gatgatgttt tcccaattct tcttccccat
     3181 gcgttgtccc gcttggggaa tggcgactat gttataagtg ggcggtttta tgataggttc
     3241 gtcggtccga gatatcaaat gtgggtagcg cgttttacat cgcaaggtaa catgatctgg
     3301 agcaagtcgt tcggtgatgt tttggatgac gaagggttgg gcgtagaagt aataggaaat
     3361 tcaatttaca gtaccggcta tactatgacg gattctcttt ctaccgacat ctacctatta
     3421 agactagatt tgaatggaga aatcaggtag ctacacgcat attactgata tgggaaattt
     3481 atgttacgcg gtattatgtg ctcaagtaag tagtgttcat tcatcgaatc tgatcagagt
     3541 gtgaatgtga agaagttata atgtttcgat gaatcttgat tttagaagca ctgatattgt
     3601 caatgtatag tgtggaaaat ccacgagtag aatttatatc ggatgtccac gaattgatac
     3661 ctacgtaatt ataatctttt aaattaagaa ttttatgaaa cggtgttcaa ctctgagttc
     3721 atgacaccga ctacgatcgg taaagcaaat cattgatata gatcgctaat actgagccaa
     3781 cgctttgatg tattgtgcgc gttccatggc cgatgggtcg tctttggatt tttgactcat
     3841 gctgccgcgg atatggctca atgcttggca ctcgtgaata tccatccaaa gttctatatc
     3901 gcgcagtaat tctgtaatgt gagagatgcc gtttttgagt aaggcggaac acaccatcgt
     3961 taccgaagcg ccggccataa tcattttgat cgcatcttcg tgggaatgta tgccgcttgt
     4021 cgctgcaatc ggggcatgca cctgaccgta caggatcgct gtccagcgca aagcaagccg
     4081 taaatcggtt gagtcactaa gattgatatt aggaacaact ttgagtgaat taagattaaa
     4141 atcgggttga taaaagcgat taaaaagaac gacaccgtcc acgcgcgttt cgtccaaccg
     4201 ggaaacaaag ttagctaacc ctgtgatatg agggctgagc ttgacagaaa cagggatctt
     4261 aagatttttg acggaacgga cgacgtgtat gaattgatct tctatttgcg cagaattctt
     4321 tttaggatcg gcggcgatgt gatacagatt gagttccagc ccgtcagcgc ctgctgcctc
     4381 aatggatttg gcgtattgta accaaccgtt atccgaagat ccgttgaggc tggcaatgat
     4441 aggaatttta acaatttctt tggctttgcg gatatgctca agataagcat caggattata
     4501 atgcgactga ttgagttcgc gtaagtaatc aaaggagttt cggaagtcct cacttatttt
     4561 ggaggcttct tgtactagct ccatcgcatc gtgataaatc tgctcttcaa acaacgaaaa
     4621 tagtacgaca gccgccgcgc ccgcatcttc cattttcctg atattgtcta aatgtttaga
     4681 tagcggagaa gctgaaggaa caatcgggtt ttttaacgta aaacccatgt acgaagtgga
     4741 taaattcgcc ggcatatatg aaaacctcga agatggttgg acgtggtaaa ttaaaacatg
     4801 cggcatttat atacaaggat gttccttggt gttgacaatt acggaatttt ttacgaccat
     4861 gcgacgttct aagaatgtat ctcagagata ccaatgttct tttgcaatat ggggacgaac
     4921 tggattcgac gggtataggg tgctttgggt cgcatgccgc gctttttctg tgtgcgcgta
     4981 aaataagcgg aaaccaatac tcaacaataa cgcatacgcg ttggctgcct aaggattaat
     5041 ttcctgcagt ccgtttcggc catctcccgt tgatcggagt tagctgagat gtcatttttg
     5101 atcgactagc gaagggccaa tgctcggagg tctgacgcga aatactatcg agctcgccgg
     5161 tttgcttttt ggacgttttt tatgagccgg tt
//
LOCUS       DAOQNQ010000229         5091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_6762_length_5091_cov_4.766283, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000229 DAOQNQ010000000
VERSION     DAOQNQ010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6762_length_5091_cov_4.766283"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="PK754_12225"
     CDS             <1..1288
                     /locus_tag="PK754_12225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNB10317.1"
                     /translation="PIYTHGKYGRLAMRAGMWAYDLLSFDKSLPRFSVYTHRAVRARY
                     PGINAQSLLGGVSYFDAQAIYPERLVLDNIHSAVRYGAEVYNHTEVTGFLKSKERITG
                     VQTRNTLTGEEAEYTADMVINTSGPWVDHILKHTEKNIPPQLGGTKGSHIVIRNTIAL
                     QDALYVNARRDGRPFFIIPWLDYILIGTTDLRYEGHPDTVFPESHEIDYLIEEATHVM
                     PELISGRDDIVFSFCGIRPLPRDERRHTGAITRRHGILDHQREGAEGLISLIGGKLTT
                     YRSAAEQMVDHIEKSLGIAMQPCRTATEPMHPLMHHDTELLPLTAMLGSDAGAAAQMA
                     QQKNWSLSPVAPDYQTSEAQIRYAVQEEWARTLSDVMLRRTRLAYSPDNGLPVAHGVA
                     SIMGSALAWPQERTTAEIQAYTAYVNAYLQAHRHRS"
     gene            1404..3479
                     /locus_tag="PK754_12230"
     CDS             1404..3479
                     /locus_tag="PK754_12230"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HNB10318.1"
                     /translation="MEINTPVWAGTDEALNLAEKARHAIRASRGKLSAYKLTTYSKSF
                     IDLYKRKKNAFEMKSASLVHAEYYWRQPDEVREIEIARRHIDDFFYTDYNIEHLPIID
                     DLTRARITVGRASVAGPLTEDAAVYYDFSLAGDTVIGGVAVHQLRCKPKTEYEPLMDG
                     VVYIAQETFQVVGFEIQFNKAVKFFPPPDLLQVRVMYAETPSGVWLPKHIQWGMQFNI
                     TFPWPAQAQWRSETMVYETAINPEIPSAVYRGKAVETREDAYFRDSAFWADNEKLPMT
                     EDEAQGFSNLGQLPWNLKILNPDLKGYRKRQRQKDIDWGFQLIPDIRYNRVEGFFTGA
                     KIEFDDLAYKRIVRNLTIKTKVGYGFADEAVKYSVEAQKKWLDKRFYVGGKYYDDLAY
                     KEFTNDGSVLNNSLTSLVYRYDIYNYFYVRGYQTYTGVRPFRGARVEFAYTDRWDSSA
                     TKNAHYALIKSMYKDFDPVYPINDGRLRRITVSANYTIGTGTGIAPRTPYWIFDASID
                     HANPNFLKSDFHFTLYNFSARFHIPTTRRGSLDGKAALGLGTDRLPNQYLFHLYGGTT
                     PYVLKTVDFREPTPNHFQGNRMAALTMEHNFGGTLLERTDLPFLRDGYVDCIPTFSIG
                     FVHASKKTLENLRQNKIQYLRRPLVEAGFAVGDIYRIVRLDFTWRLTQRSKGNRNFAA
                     TMAVLVQRF"
     gene            3589..4392
                     /locus_tag="PK754_12235"
     CDS             3589..4392
                     /locus_tag="PK754_12235"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HNB10319.1"
                     /translation="MEKRLAGQKALVTGANSGIGAGIVQRLAAEGADVMINWFANEDA
                     TKALVDKINKAGAGKAIAFRADVSNEDQVNAMYEAMFKEYGTIDILVNNAGIEIHNFL
                     ENVTKAEWDKVIGVNLTGNFLCSRSAIRQFLKNENRPNSRSRGKIVNISSVHQVIMWA
                     GFSSYCATKAGINLFAQSVAQEYAEKKIRINQVAPGAIKTPINQNVWSDPKGMADLMT
                     KMPYGRMGEVDDVAAAVAFLCSDEADYITGTTLYVDGGMCLYPEFRFGG"
     gene            4411..5010
                     /locus_tag="PK754_12240"
     CDS             4411..5010
                     /locus_tag="PK754_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10320.1"
                     /translation="MEDSKETSRNNPSETPETTRKYEREISDYIHFQIEETQAPESAF
                     EQKVVPESIREQGFWRRFHVRRRIFVSRTADFFKDTKKMVVVWIIIMALVAIGSYFGW
                     DREMVGGAVVFFGLISSAFSWLAAVILGGIGLIPFIGPVLVAILSSSLLWIINGLGYF
                     VSVVAIRAGHGKAVLNYRLLVIIFLCGIVTGYVIAKIVH"
BASE COUNT         1307 a         1142 c         1294 g         1348 t
ORIGIN      
        1 gccgatatac acacatggta aatatggccg attagccatg cgtgcgggga tgtgggcata
       61 cgatcttttg tcgtttgata aatcgcttcc gcgtttttcc gtatacaccc atcgcgccgt
      121 gcgtgcgcgt tatcccggca tcaatgcgca aagtcttctc ggcggtgttt cttattttga
      181 tgcacaggcg atttacccgg aacgtttagt tttggacaat atccattcgg ctgttcgcta
      241 cggcgcagaa gtatataacc atacggaagt taccggattt ttgaagtcaa aagaacgcat
      301 aaccggagtg caaacacgaa atacgctcac cggtgaagaa gcagaataca cagccgatat
      361 ggtgatcaat acgtccggtc cttgggtgga tcatattctc aaacacaccg aaaaaaatat
      421 accgccccaa ttaggcggta ccaaaggaag ccatatcgtc atacgcaata cgatcgcatt
      481 gcaagatgcg ctttatgtta atgcccgtcg ggacgggcgt ccgttcttta tcataccatg
      541 gttggattac attctcatcg gcacgacgga tttgcgctac gaaggtcatc cggatacggt
      601 gtttcctgaa tcgcatgaga ttgattacct catcgaagaa gcaacccatg tcatgcctga
      661 gttaatctca gggcgtgacg atattgtttt ttcattttgt gggattcgcc cgcttccacg
      721 tgacgaacgt cgtcataccg gagccattac gcggcgacat gggattttgg atcatcaacg
      781 cgaaggcgcg gaaggcttga tttcgctcat cggcggaaaa ttaacgacat accgtagcgc
      841 ggcggagcaa atggttgatc atatcgaaaa atcgcttggt attgctatgc aaccgtgccg
      901 tactgcaaca gaacctatgc accctttgat gcatcacgat acagaactgt tgccattgac
      961 agcgatgctg ggatccgatg cgggagcggc cgcgcaaatg gcgcagcaaa aaaattggtc
     1021 attgagcccg gttgctcccg attatcaaac cagcgaagcg cagatacggt atgccgtgca
     1081 agaagaatgg gcacgcacac tctccgatgt aatgttacga cgtacgcgac tggcttatag
     1141 tccggataac ggattgccgg tggcgcatgg tgtggccagt attatgggga gcgccttggc
     1201 gtggcctcag gagcgaacca cagcggaaat acaagcgtac acggcgtatg tcaacgccta
     1261 tcttcaggcg catcgtcatc ggtcataagt cttgattgac atacgcgtct tccttacatt
     1321 acctcccgat atcgtttctc acttttcagc ctgtagaatt attgatgaag ttttttcgat
     1381 tattattttt aataaccgtg ctcatggaaa ttaatacccc ggtttgggcc ggtacggatg
     1441 aagcgctgaa cctcgccgag aaggcgcgtc atgcgattcg cgcatcacgg ggtaaacttt
     1501 cagcctacaa acttaccacg tacagcaaat cttttattga tctctataaa cgcaaaaaaa
     1561 atgcgtttga aatgaaaagc gcatcgctgg tgcacgcgga atactattgg cgccaacctg
     1621 atgaagtgcg agagatcgaa atagctcgcc gccacattga tgactttttt tatacggatt
     1681 ataatatcga acatcttccg attattgatg atcttacccg tgcgaggatc accgtgggtc
     1741 gcgcatcggt agcaggcccg ctgacggaag atgccgcggt ttactatgat ttttcgctgg
     1801 ccggggacac ggtcatcgga ggtgttgcgg tccatcagtt acggtgtaaa cccaaaacgg
     1861 aatacgaacc cttgatggat ggggtggtgt atatcgcgca ggaaacattt caagttgtgg
     1921 gttttgaaat acagtttaat aaagccgtca aatttttccc gcctccggat ttattgcaag
     1981 tgcgcgtcat gtatgcggaa actccatcgg gtgtttggct gccgaagcat attcagtggg
     2041 gaatgcagtt caatattaca tttccgtggc cggctcaagc gcagtggcgc agtgaaacga
     2101 tggtttacga aacagcgatc aatcctgaaa taccgtcggc cgtgtatcga ggcaaagccg
     2161 tcgaaacgcg tgaggatgcg tatttccgcg attccgcatt ttgggctgat aatgaaaaat
     2221 tgccgatgac ggaggatgaa gcacaggggt ttagcaatct tggccaattg ccttggaatc
     2281 tgaaaatcct caatcccgat ttgaaaggat accgcaaacg tcagcggcaa aaggatattg
     2341 attgggggtt tcagctcatt cccgatatcc gctacaatcg tgtcgaagga ttttttaccg
     2401 gtgccaaaat agaatttgat gatttggcgt acaaacgtat tgtcaggaat ctgacgatca
     2461 aaaccaaagt cggatatggg tttgcggatg aagcggtgaa gtattccgtc gaagcgcaaa
     2521 aaaaatggtt ggacaaacga ttttatgtcg gaggtaaata ttacgacgat cttgcgtaca
     2581 aagaatttac caacgatggc agcgtactca ataactccct gacgagtctg gtgtaccgtt
     2641 atgatattta taattatttt tatgtacgcg gttatcagac ttataccggg gtcaggcctt
     2701 ttcgcggtgc acgggtagag tttgcttaca ccgatcgctg ggattccagt gcgaccaaaa
     2761 atgcacacta cgcgctcatc aaaagtatgt ataaagattt tgatccggtg tatccgatca
     2821 atgacggccg tttacgtcgt atcacggtga gtgctaacta tactatcggc acgggaacag
     2881 gtatcgctcc gcggacaccg tactggattt ttgatgcgag tattgatcat gccaatccca
     2941 attttctgaa aagcgatttt cattttacgt tgtataattt ttccgcgcgg tttcatatcc
     3001 ccacgacgcg gcgcggttca cttgacggga aggccgcact gggtctgggc acggaccgat
     3061 tgccaaatca gtaccttttt catctttatg gcggcacgac gccgtacgtt ttgaaaacgg
     3121 tagatttcag agaaccgacg cccaatcatt ttcaaggcaa tcgtatggcg gcgttgacga
     3181 tggaacacaa tttcggcggc acgttattgg aacgtacgga tttgccgttt ctgcgcgacg
     3241 gatatgtaga ttgtataccg acgttttcca taggttttgt acatgcttca aaaaaaacat
     3301 tggaaaattt gcgacaaaac aaaatccagt atctaagaag acctttggtc gaagcggggt
     3361 ttgcggtcgg cgatatatat cgtattgtgc ggctggattt tacctggcgc ttgacgcaac
     3421 gcagtaaagg aaatcgtaat tttgccgcca cgatggccgt cctagtacag cgcttctgac
     3481 gaaaaagggt ttgtttcata tcgcaaatag aactaacttt ttcgcacttt ctgaatacct
     3541 gtaatttttt tcatatccaa aatcgtctaa ccaaacggga gtatcgtcat ggaaaaacgt
     3601 cttgccggcc aaaaagccct cgtcaccggc gccaatagcg gcatcggtgc gggcatcgta
     3661 caacgtcttg ccgccgaagg tgcggatgtt atgatcaatt ggtttgccaa cgaagatgcg
     3721 accaaagcgc tggttgataa aatcaacaaa gccggcgccg gcaaagctat cgcgtttcgc
     3781 gcggatgtat ccaacgaaga tcaggtcaat gcgatgtacg aagcgatgtt caaagaatac
     3841 ggtaccattg atattcttgt gaataatgcg ggtatcgaaa ttcataattt tttggaaaac
     3901 gtcaccaaag cggaatggga taaagtgatt ggtgtcaatt tgacgggtaa ttttctctgc
     3961 tcccgcagcg cgattcgtca atttctgaaa aacgaaaacc gtcccaattc ccgttcacgc
     4021 ggcaaaattg tcaatatcag ctccgttcac caagtgatca tgtgggccgg attttccagt
     4081 tattgcgcga cgaaagccgg tatcaatctt ttcgcgcaat ccgttgcgca ggaatacgcc
     4141 gagaaaaaaa ttcgaatcaa tcaagtggcg cccggtgcga tcaaaacacc gatcaatcaa
     4201 aatgtatgga gcgatcccaa aggcatggct gatcttatga ccaagatgcc gtacggacgt
     4261 atgggtgaag tggatgatgt cgccgcggcc gttgcgttcc tttgctctga tgaagcggac
     4321 tatatcaccg gtacgacctt gtatgtggac ggcggtatgt gcctctatcc ggaattccgt
     4381 ttcggcggtt gataacgaga actaccgagt atggaagatt ccaaagagac atcgcgcaat
     4441 aacccgtcgg aaacccctga gaccacgcga aaatacgagc gtgagatttc ggattatatc
     4501 cactttcaga tcgaagagac gcaggctccg gagtcggcgt ttgagcaaaa agtggttccg
     4561 gagtctattc gcgaacaggg tttttggcga cgatttcatg ttcgccgcag gatatttgtt
     4621 tcacgaaccg ccgatttttt caaagatacg aaaaaaatgg tggtggtctg gatcatcatc
     4681 atggcattgg tggcgatcgg ctcgtatttc ggttgggatc gtgaaatggt cggcggtgcc
     4741 gtcgtttttt ttggattgat ttcaagtgcg ttttcctggc ttgctgcggt gatcttgggc
     4801 gggatcggtt tgatcccgtt tatcggaccg gttcttgtgg cgattctcag ttcatcgctc
     4861 ttatggatca tcaatggact cggttatttt gtctccgtgg tcgctatccg cgccggtcac
     4921 ggtaaagccg tactcaatta ccggttgttg gtcattattt ttttgtgcgg gatcgtcacg
     4981 ggctatgtca tagccaagat cgtgcactga ccaaaaaaga attggaaatt ttaaagcgtt
     5041 tacgtatgtt gggcgcaatt tgcaagtaat tctaattttt ttataaactg t
//
LOCUS       DAOQNQ010000230         5088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_6771_length_5088_cov_5.892112, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000230 DAOQNQ010000000
VERSION     DAOQNQ010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6771_length_5088_cov_5.892112"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PK754_12245"
     CDS             <1..624
                     /locus_tag="PK754_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10321.1"
                     /translation="KAPVHAIAVQWPDSVIVRIDRMIARRVPVVADVQITCEAGFILV
                     GGMQLMPDSVTVTMPWSYRDSAVVIGTASHRWENLNSDVRTELRLTAHPDRRINVLQS
                     TTSLFLDVQPLGEIVLDNLPVRLINVPPFTTVAVQPSTFSVRVRGGVDYLSTLPKESV
                     VGEIDYQKEYNSVQPRLDIRVPADLTWTQVTPSRFNLVRIEDDSLER"
     gene            605..1639
                     /gene="tsaD"
                     /locus_tag="PK754_12250"
     CDS             605..1639
                     /gene="tsaD"
                     /locus_tag="PK754_12250"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007788833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNB10322.1"
                     /translation="MIASNDDLITLAIETSCDDTSAAVLRGSSVLSNIVSSQIDGLRF
                     GGIVPELASRAHQANIVPVIRAALSAAYITPDDIGLIAVTKGPGLIGSLLVGVNFAKA
                     MAFGLQKPLVGVNHIEAHVLASFLEKDKPVFPFIALIVSGGHTLLVEAQNPGQYRILG
                     ETVDDAAGECFDKTARLLGLTPVEGALMAGPVIDRLASQGNPKAIAFPRPMLQSGDLN
                     FSFSGLKTSVLHFVQKQNQPLAQNLLADACASFQEAAVDVLVGKTRAALKTTGICQCV
                     LSGGVAANSRLRARLTEVCAQDGVQLFMPARQYCTDNAAMIGITGLMHYRMGRTDNLG
                     LSPKASLRIA"
     gene            1731..3440
                     /locus_tag="PK754_12255"
     CDS             1731..3440
                     /locus_tag="PK754_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10323.1"
                     /translation="MPKSFFLVALVVVSAGCNPFNQEQITTLSCYEGQPAFSPNGDVM
                     AFVTTQTGDRHIWLYDLGERITIPVTVNEGVDEHPSFAPDGERIIFTSRLNGQNDLWI
                     VSPNDELIQVTKTDSVDEIFPVYSPDGMYIWYIAISKSAADGGYAIHRTEARNFSKSE
                     VIYRSRGPLEKITIAKDKIVFGEKNEASWVLMRLDPATGSVARWSDGYRDARHAALSP
                     DGKHLFFSSDTNGYAQIFFTRADTFNPVLISGESKHLDFPVWTPDGHAMAYQAETSYD
                     IRRIEVKTQMEQMLIETNGDDRSPVMLADGKTLLYVSNAEGRYALYARNLSANKAIRI
                     AYWPDADVTECDLRNDGTAAVVSVVRGGLSTLYEMPITQANLIEEYAAGKSAYALFQD
                     SVHRYAAHYAIDGKAITYVSLKGGSPDIWVYDTATKKEKQLTIDMRTEANPVFSNDGA
                     YIYFDADWAGRWSIWRVPVSGGMPSPVTRDKTPYGSDREPSWSPDGQWLAITRTWYDD
                     ADIWLYKTDEGEKTSRTLSKDNNHQERRGRWTHDGKYMIYESGGNTDIWMQSVAKLLE
                     ATK"
     gene            3472..>5088
                     /locus_tag="PK754_12260"
     CDS             3472..>5088
                     /locus_tag="PK754_12260"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNB10324.1"
                     /translation="MPVIGSPAGQWNPEKYQKNRSFMERLLNALRQDGEAIKRGGGQK
                     NIDKQHEKGRLTARERIVKLLDPNAHFMELGLFAAYEMYTDFGGCPSAGCVSGIGLIH
                     GKETVIVAHDATVKAGAHFEMTVKKTLRAQEIAMKNFLPIVYLVDSAGVFLPLQDQVF
                     PDENHFGRIFYNNARMSAMGITQVAAVMGPCVAGGAYLPVMCDKFIMVQGASLFLAGP
                     ALVKAAIGQVIDAETLGGATTHNAISGTADYHEPNDGAALDRIRDILSKIAERPKANF
                     NKIAAVAPQWAMDEIAGILPEGLGQYDMREIIARLIDGSDFNEYKATYGKTVVCGTAR
                     VGGYAVGFVANQKLVQRTVKGEMQMGGVMYNEAADKAARFVMNCNQDGIPLIFMHDVN
                     GFMVGRDAEYAGIAKDGAKLVNAVANSVVPKITIVIGGSYGAGNYAMCGKAYDPRFIF
                     AWPTANISVMGGAQAASTITEIRLAGKQVSEEEKKKLYEEIRSNYEKQGNPRYAAARL
                     WVDAIIEPTETRDTLIRCLDAASNNPNVPPP"
BASE COUNT         1265 a         1203 c         1370 g         1250 t
ORIGIN      
        1 aaagcgccgg tgcacgccat cgccgtacaa tggccggatt cggtcatcgt acgaattgac
       61 cgtatgatcg cgcgccgcgt tccggtggta gcggatgtgc agatcacatg cgaagccggt
      121 tttatattag tcggcggcat gcagctcatg ccggattcag tgacggtgac aatgccctgg
      181 agttaccgtg acagtgcggt tgttatcggc accgcttcgc atcgctggga aaatctcaat
      241 agcgatgtgc gcacggagtt gcgactcaca gcgcatccgg atagacgcat caacgtactc
      301 caatccacaa cttcactttt tctggatgta cagcctttag gcgaaatcgt actggataat
      361 ttgcccgtgc gattgatcaa tgtgccacct tttacgacgg tggcggtaca accgtccact
      421 ttttccgtac gtgtacgcgg cggtgtggac tatctttcaa cattgcctaa agagagcgtc
      481 gtcggagaaa ttgattatca aaaagaatac aattccgtcc aaccgcgtct ggacatccgc
      541 gttcccgcgg atcttacctg gacgcaagtc acaccgtcgc gatttaacct cgtgcgtatc
      601 gaagatgata gcctcgaacg atgatctgat cacactggcg atcgaaactt cctgcgatga
      661 tacgtcggcg gcggttttgc ggggatcgtc ggtattatcc aatatcgtat cgtcgcaaat
      721 tgacggactt cgtttcggag gcattgtccc ggaactggct tcccgcgcac atcaggccaa
      781 tatcgtaccc gttatccggg ccgcgctttc ggcggcctat atcacgcctg atgacatcgg
      841 attgatcgcg gtgaccaaag gccccggcct gatcggcagt cttttagtcg gtgtcaattt
      901 tgccaaagcg atggcctttg gtttacaaaa accgctcgtc ggagtcaatc atatcgaagc
      961 gcatgtattg gccagttttt tagaaaaaga caaaccggtt tttcccttca tcgcactgat
     1021 cgtgtccggc gggcatacgt tattggtgga agcgcaaaat ccgggtcaat accgtatttt
     1081 gggtgaaacc gtagatgacg cggccggtga gtgttttgat aaaaccgcac gattgctagg
     1141 tttaacgccg gtagaaggcg ctttgatggc cgggccggtt atagatcgcc ttgcatcgca
     1201 aggcaatcct aaagcgatcg cctttcccag accgatgctc cagtccggcg atttgaattt
     1261 tagtttcagc ggcttaaaaa cctcggttct ccattttgtg caaaagcaaa atcaaccgct
     1321 tgcacaaaat ttgctggcgg atgcgtgtgc gagttttcag gaagccgcag tagatgtttt
     1381 agtcggaaaa acacgtgcgg ccttaaaaac gacgggtatt tgtcaatgcg tattatccgg
     1441 cggtgttgcg gccaatagtc gcttacgcgc caggcttacg gaggtatgtg cacaggacgg
     1501 agtacaacta ttcatgcccg cccgccagta ttgtacggat aacgccgcga tgatcggtat
     1561 cacgggtctg atgcattatc gtatggggcg taccgataat ttgggtctat cgcccaaagc
     1621 ttcgttgcgt atcgcctgag gggctttttc aaaaaataac tgtagagctt ttctatcgtt
     1681 ttgtgtatgt tgcgtcagca atattttcat ttgaaatcgt aacgtcccac atgcccaaaa
     1741 gtttttttct tgtcgcactg gtcgtcgtat cagccggttg caatcccttt aatcaggaac
     1801 aaattacgac cttatcgtgt tatgaaggtc agccggcatt ttcaccgaat ggcgatgtga
     1861 tggcatttgt cactacgcaa accggcgatc ggcacatttg gctttacgat ttgggcgaac
     1921 gtattacgat ccccgtaacc gtcaatgaag gtgttgatga acatccgtcg tttgcacctg
     1981 acggagaacg cattattttc acctctcgtc tcaacggaca aaacgacttg tggattgtct
     2041 ctcccaatga tgaattgatt caggttacca aaacggattc ggttgacgaa atttttccgg
     2101 tgtattcgcc cgatggcatg tatatttggt atatagccat aagcaaatcg gcggccgacg
     2161 gcggatatgc catccatcga accgaagccc gtaatttttc caaatcagaa gttatttatc
     2221 gcagccgcgg gccattggaa aaaattacta tcgctaaaga caaaattgtg tttggtgaaa
     2281 aaaacgaagc atcgtgggtt ttgatgcgtt tggatccggc gacagggtcc gtcgcacgat
     2341 ggagtgacgg atatcgtgat gcgcgtcatg cggcgctttc tccggatggc aagcatttgt
     2401 tcttttcttc cgataccaac ggctatgcgc agattttttt tacacgtgcc gatacgttca
     2461 atccggtgct gatcagcggc gaatccaaac atttggattt tccggtgtgg acgccagacg
     2521 ggcatgcgat ggcctatcag gcagagacct cgtatgatat ccgccgtatc gaagtcaaaa
     2581 cacaaatgga acaaatgctg atcgagacca atggcgatga tcgttctccg gtcatgttgg
     2641 cggatggcaa aactctgttg tatgtgagca atgcggaagg ccgctacgcg ctgtatgcgc
     2701 gtaatctgag cgccaataaa gcaattcgca tcgcctattg gccggatgcc gatgtgaccg
     2761 aatgcgactt gcgaaatgac ggcacggccg cggtggtttc cgtggttcgc ggcgggttat
     2821 ccacgctgta tgaaatgccc atcacgcaag ccaatctgat tgaggagtat gcggccggaa
     2881 aatcagccta cgctttattt caagacagcg tacaccgcta tgcagcgcat tatgctatag
     2941 acggcaaagc gatcacatat gtttccctaa aaggcggcag tcccgatatt tgggtgtacg
     3001 ataccgcgac caaaaaagag aaacagttga ccatagatat gcgaaccgaa gcgaaccccg
     3061 ttttttcaaa cgatggcgcg tatatttatt ttgacgccga ttgggccggt cgctggagca
     3121 tttggcgcgt gcctgtttcc ggaggaatgc cttcacctgt gacgcgcgat aaaacgccct
     3181 acggctccga tcgggaaccg agttggtcac ccgacggcca atggcttgcc atcacgcgta
     3241 cgtggtatga cgatgcggat atttggcttt ataaaaccga cgaaggagaa aaaacgtcgc
     3301 gtactttgtc caaagacaat aaccatcaag agcgtcgcgg tcgatggacg catgacggaa
     3361 aatatatgat ctatgagtca ggcgggaaca ccgacatctg gatgcaaagt gtggctaaac
     3421 ttttggaagc aacgaaataa atcatcagcc tgtgtgtata aggagtgcag tatgcccgtg
     3481 ataggatcac cggccggtca gtggaacccg gaaaagtatc agaagaatcg tagctttatg
     3541 gaacgtttgc tgaatgccct tcgtcaggat ggcgaggcga tcaaacgcgg tggcggccaa
     3601 aaaaatattg ataaacagca tgaaaaagga cggctcacgg cccgtgagcg tattgttaaa
     3661 cttttggatc caaatgccca tttcatggaa ctggggcttt ttgcggctta cgaaatgtat
     3721 actgattttg gcggttgccc gtcggcgggt tgtgtgtcag gcataggttt gattcacggc
     3781 aaagaaactg tcatcgttgc acatgatgcg acagtcaaag ccggagcgca ttttgaaatg
     3841 acggtcaaaa aaacattacg cgcgcaggaa attgcgatga aaaattttct gccgatcgtg
     3901 tatctcgtgg attccgccgg tgtattcctt ccgcttcagg atcaggtttt tcccgatgaa
     3961 aatcactttg gtcgcatttt ttataacaac gcccgcatga gtgcgatggg cattacgcaa
     4021 gtagcggctg tgatggggcc gtgcgtcgca ggcggtgcct acctgcccgt aatgtgcgac
     4081 aaatttatca tggtgcaggg tgcgagcttg tttcttgccg ggccagcgct agtcaaagcg
     4141 gccatcggac aggtgatcga tgcggaaact ttaggcggag cgaccacgca caacgcgatc
     4201 agcggcacgg cggattatca tgagcccaat gatggcgccg cattggaccg aatccgggat
     4261 atcttgagca aaatagccga gcgccccaaa gccaatttca acaaaatcgc tgccgttgca
     4321 ccccaatggg cgatggatga aatcgcaggt attttacccg aaggtctagg tcaatatgat
     4381 atgcgtgaaa tcattgcacg tctgattgac ggatcggatt tcaatgaata taaagcgact
     4441 tacggcaaaa ccgttgtttg cgggacggca cgcgtcggcg gttatgcggt cggatttgtg
     4501 gccaatcaaa aactggtcca acgcacggtc aaaggcgaaa tgcaaatggg cggcgtgatg
     4561 tacaatgaag cggcggacaa agcggcgcga tttgtgatga attgtaatca ggacggcata
     4621 ccgctgattt ttatgcatga tgtaaatggt tttatggtag gccgcgatgc ggaatatgcg
     4681 ggcattgcca aagacggcgc gaagcttgta aatgccgtag ccaactccgt agtgcccaaa
     4741 attacgatcg tgatcggcgg aagttatggt gcagggaact atgccatgtg cggaaaagca
     4801 tacgatccgc ggtttatttt tgcctggcct acagcgaata tttccgtgat gggcggagca
     4861 caagccgcat cgacgattac agaaatccga ttggccggta aacaagtcag cgaagaagag
     4921 aagaaaaagc tttacgaaga aattcgttct aattatgaaa aacagggcaa tccgcgttat
     4981 gcggcggcac gtctctgggt tgatgcgatc atcgaaccta cggagacgcg cgacacgttg
     5041 attcgatgtt tagatgcggc cagcaataat cccaatgtgc cgccccct
//
LOCUS       DAOQNQ010000231         5063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_6836_length_5063_cov_6.143970, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000231 DAOQNQ010000000
VERSION     DAOQNQ010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6836_length_5063_cov_6.143970"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="PK754_12265"
     CDS             <1..1156
                     /locus_tag="PK754_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10325.1"
                     /translation="MKNFSGTFGLHVQRLTSGTRELRNLHDPSGMYNDVSPGVLDEKD
                     FYSHEYTTILFLPRLGVNIQLNEMLSAYSHYGWYSTPGGLQRRYVGSEFLEKTVLAPS
                     VETIIGNSKLKPVESELFDLGARVNFSSPSSMGAQFYIQEIKNIVDYTKVVAEPNGYI
                     SYYNGPKAGIVGFAADITLIPHENFFIIWNYGAQTSEFELGSNVSSYHFAWLGFNTAN
                     FAVPVSHVRTWQFGQQATLHFDNSAAEPLLKGTFASMEWHFKDGSFYTPTTMAPIQAV
                     GYNNQYPVSKKNSSETGSLWTIDIKAGKSFVWKNHTATLFIECTNLYNNKNPTQVFSS
                     SGKPDDNGFLDSPYFLDYQKRIRPQYKAHFRNGLMYEPGRELRMGIEISM"
     gene            1270..3228
                     /locus_tag="PK754_12270"
     CDS             1270..3228
                     /locus_tag="PK754_12270"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNB10326.1"
                     /translation="MPNSSNNSDSSTVSVSPVIIATTAITSFTITFALYLNTLAPTVT
                     FEDSGELIAAAAHLGIPHQPGYPLWTLLSHLFTWLPFGNIAYRVNMASAFFSASGAAL
                     IAWTFLLWLRLTYIDILKKSSLGFLFSVLTAFLTATSSEAWEQSVISEVYGLHIFFTG
                     IILLLTTLWLSETGNNERSRYFFLLSFVLGLSLSNHTTTLVLLPPLVMLAFITDRNFI
                     LNLKRIIIATGFITLGLSCFIYLPLASARNPMMDWGNPETWTNFWRVIARHQYQLNET
                     QTLVKFISQLKTYFSLLGEQWFSPILVLVLPALVFLWKRHRSLLLFVLVFWFFTAPVT
                     TYLTNFDVTTGNPLVSAENKALVSVFYIPSYMIVAALVALGTISLVEQWPSRVTYIAA
                     GLLAIACLWHLPENYKRNNMHQYRFTECFAENIFSVTDSNALVIGNWDPLVFPFFYYQ
                     QVEKRRPDLFMIDQELLRRSWYIQMLRDHNPESTELIKKEIDDFLDAVKPFEAGEKFD
                     GNYIQRRYERMINALIDNWMQSGRSVYLTYDPPKGIAEKYYKESVLTAIRLRTDMIRP
                     TSLDHKNIQTEIFTDEAINKDRMARFFVTYYGRLFYARAAIMESLNQKENALTLYERA
                     AQLLKNQSSYTPTLENAIRRLQAKAATP"
     gene            complement(3225..3770)
                     /locus_tag="PK754_12275"
     CDS             complement(3225..3770)
                     /locus_tag="PK754_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10327.1"
                     /translation="MCPTDDKENASKIPFNGARWADSVVGMSSEYYPAVASWNAVEAL
                     GEPNVYPDYGDIPYAWTSQTQDGQREYLELGFFSNPQPVASIAIFETYNPGAIDTLYV
                     MNPNNNLWEIVWSDSAHDRGDSSRIFIANFAKTSFNVSVIRIAINSPKVISWNEIDAV
                     AMAQEEIPPFYDDSTGFYSKD"
     gene            complement(3901..4548)
                     /locus_tag="PK754_12280"
     CDS             complement(3901..4548)
                     /locus_tag="PK754_12280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB10328.1"
                     /translation="MKNKKITVWVVEDNDAMRTTYAELINSASNMRCIGNFDAAEEAI
                     KALSAQEHPDVIMMDIELPGINGIEAVRRIKEKAPAINIIMQTVYEDNEKIFQSICAG
                     ATGYMLKRTPAEKMIESIYEAHTGGAPINAQIARKVLTMFAQEMRPSGADYNLTVREK
                     EILQNLVSGLTIKMIADKLSISAMTVGTHVKKIYAKLQVSSRGEVVAKAIQEKLV"
BASE COUNT         1382 a         1121 c         1009 g         1551 t
ORIGIN      
        1 aatgaaaaat ttctcgggaa cattcggtct gcatgtccag cgcctaacct caggtacacg
       61 agagcttcgt aatcttcatg atccttccgg catgtataat gacgtatccc caggggtatt
      121 agatgaaaaa gatttttact ctcatgaata taccacgata ctatttttac cacggctagg
      181 agtaaatata caacttaacg aaatgttatc ggcttacagc cactatgggt ggtacagtac
      241 acccggaggg ttacaacggc gttatgtcgg atcggaattt cttgaaaaaa cggttttggc
      301 gccatcggtt gaaacgatca tcggtaattc gaaattaaaa cctgtggaat ccgaattatt
      361 tgatttggga gcacgtgtaa atttttcttc tccttcatcc atgggcgcac agttttatat
      421 acaagaaata aagaacatcg ttgactatac caaagtagta gctgaaccga acggctatat
      481 ttcgtactat aacggcccga aagcaggaat tgttggtttt gcagcggata ttacgctgat
      541 accccacgag aattttttca ttatttggaa ttatggcgca caaacttccg agtttgaatt
      601 agggtcaaat gtttcaagtt accacttcgc ctggcttggt tttaacactg ctaattttgc
      661 agtgccggtt agccatgtaa gaacatggca attcgggcag caagcgacac ttcattttga
      721 caacagcgca gcagaacctt tgctcaaagg cacttttgca agtatggaat ggcattttaa
      781 agacggatca ttttataccc cgaccactat ggctcctatt caagccgtcg gatataataa
      841 ccaatatccc gtatccaaaa aaaacagcag tgaaaccggc agcctttgga ctattgatat
      901 caaagccgga aagtcctttg tatggaaaaa ccatacagca actttattta tcgaatgcac
      961 caacctctat aacaataaaa acccaacaca ggtattctcc tcttccggaa aaccggacga
     1021 caacggattc ttagactcgc cgtattttct agactatcaa aaaaggatac gccctcagta
     1081 taaggcacat tttcgaaatg gtctgatgta tgaaccaggc cgcgaactac gtatgggaat
     1141 cgaaatcagc atgtagttta tttatatcta aacatcttcg aaagacggtc aacttggccg
     1201 tcttttttgt ttgatgcttg tggttatgtt tgtgattatt tagatttttc aaaaattaaa
     1261 tccgatcgca tgccaaactc atcaaacaat tccgattctt caacagtatc cgtttctccg
     1321 gtcatcattg caacgacggc gataacttcg tttactatta cttttgctct atatctcaat
     1381 acgttagcgc ccacagtgac gtttgaagac agcggcgaac tgatcgccgc cgccgcacac
     1441 ctaggtattc cccatcagcc cggttatccg ctctggacgc tactttcaca tctttttacg
     1501 tggctccctt ttggcaatat cgcttaccgc gttaatatgg cttctgcttt tttttctgcc
     1561 agcggtgccg ctttgattgc gtggacattt cttctatggt tgcgtcttac atatatagat
     1621 attttaaaaa aatcatcgct cggatttcta ttctctgtgt taactgcgtt tcttaccgcg
     1681 acatcctctg aagcttggga gcaatcggtt atctccgaag tgtatggcct tcacattttt
     1741 tttacaggga taattctact tttgaccacg ttatggttga gcgaaaccgg caacaatgaa
     1801 cgctcgcggt atttttttct tttgagtttt gttttgggtc tctccctgag caaccacacc
     1861 accacacttg tacttttgcc accgctcgtg atgttggcat tcattacaga tcgtaatttt
     1921 atattaaatc tgaaaagaat aattatcgca acaggtttta taaccctcgg gctttcttgt
     1981 tttatttatt taccattagc gtcggcacga aatccgatga tggattgggg taatcccgag
     2041 acatggacca atttttggcg cgttattgct cgccatcaat accaacttaa cgaaacacaa
     2101 acgctggtta agtttatctc tcagcttaag acatattttt cgctgctcgg cgaacagtgg
     2161 ttttctccga ttcttgtctt ggtacttccg gctttagttt ttttatggaa gcgtcatcgt
     2221 tcacttttgc tatttgtttt agtattttgg tttttcaccg caccggtgac gacctatctt
     2281 accaactttg acgtgactac cggcaatccc ttagtcagcg ctgaaaacaa agcactggtg
     2341 tccgtattct atattccttc gtatatgatc gtagccgctt tagttgcctt aggaactata
     2401 tccttggttg aacaatggcc ttcgcgagtg acttatattg cagcaggatt gctggcaata
     2461 gcatgcttgt ggcatcttcc tgaaaactac aaacgaaaca atatgcatca gtatcgtttt
     2521 acggaatgct tcgccgaaaa tatcttttca gtcacagact ccaatgcgct cgtgatcgga
     2581 aattgggatc cattggtttt tccttttttc tattatcagc aggtcgaaaa aagaagaccg
     2641 gatcttttta tgatagatca ggaattactc cggcgaagtt ggtatatcca gatgctccgt
     2701 gatcacaacc ctgaatcgac cgaactgata aaaaaagaaa ttgatgattt tttagatgcc
     2761 gtgaaaccgt ttgaagccgg cgaaaaattt gatggcaatt atattcaacg gcgatacgaa
     2821 cgcatgatca acgctttgat tgataactgg atgcaatcgg gtcgttctgt ttacctcacg
     2881 tacgatcccc ccaaaggcat tgctgaaaaa tattataaag aatcggtatt aaccgcgatt
     2941 cgtttacgca cggatatgat tcgacccaca tctttagatc ataagaatat ccaaaccgaa
     3001 attttcaccg atgaggcgat caacaaagat cgcatggcgc gtttttttgt gacgtattac
     3061 ggtcgtttgt tttatgcccg ggccgcgata atggaatctc tcaatcaaaa agaaaacgcg
     3121 ctcactttat atgaacgcgc tgcacaattg ctcaaaaatc aatcctcgta cactccgacg
     3181 cttgaaaacg ccattcgacg cctgcaagcc aaagccgcta cgccttaatc tttactgtaa
     3241 aaaccggtac tgtcgtcgta gaacggagga atttcttcct gcgccatcgc gacggcatca
     3301 atttcattcc aactgattac tttcggtgaa ttgatggcga tacgaattac tgaaacatta
     3361 aatgaggttt tcgcaaagtt tgcgataaaa attcggctcg aatcgccacg gtcatgtgcg
     3421 ctatcactcc agactatttc ccaaagattg ttattggggt tcataacata cagtgtgtca
     3481 atcgcgcccg gattataagt ttcgaaaatc gcaatagaag ctacaggttg cggattggag
     3541 aaaaaaccga gttccaaata ttcacgttga ccgtcttgcg tttgcgaagt ccaagcatac
     3601 ggaatatcac cgtaatccgg atatacatta ggttctccca atgcttcgac ggcattccat
     3661 gaagctacgg ccggataata ttctgaactc attccgacaa cagaatccgc ccatcgtgca
     3721 ccattaaaag gaatcttcga tgcgttttct ttatcatcag tcggacacat gatgaaaaca
     3781 tagccgtgaa tataatagca aagattaaaa acgtacgcat gaccgcctcc tgtttttatg
     3841 aaaattagtc attagacctt ggggcttcaa tcccataagc atgggataac gcgtatcgga
     3901 ttaaactaat ttttcctgaa ttgctttagc tacaacttca ccgcgggagc ttacttgcag
     3961 tttggcgtaa atttttttga cgtgtgtgcc tacggtcatg gccgagatcg aaagtttatc
     4021 ggcgatcatt ttgatggtaa gtccggatac taggttctgg agaatttctt tttcacgaac
     4081 tgtgagatta taatccgcac cggatgggcg catttcctga gcaaacatgg ttagtacttt
     4141 gcgcgcgatt tgggcgttga ttggagcacc gccggtatgt gcttcgtaga tactttcgat
     4201 catcttttca gccggcgtgc gtttgagcat atatcccgta gcgcctgcac agatggattg
     4261 aaaaattttc tcattatctt cataaaccgt ttgcatgatg atattaattg ccggcgcctt
     4321 ttctttgata cggcgtaccg cttcgatgcc gttgataccc ggcaattcga tatccatcat
     4381 gatcacatcc ggatgttctt gcgccgacaa tgctttgatc gcttcttcgg ccgcgtcaaa
     4441 atttccgatg caccgcatat tggatgccga attgataagc tctgcatacg tagttcgcat
     4501 ggcatcatta tcttcaacta cccaaactgt gattttttta tttttcattt tctgcgtatc
     4561 cactcagtca ctatcgtatt acatgaatat agtgcaaata atatctaata acacatcatt
     4621 gtatagtatt tacatagatc tgtttgttaa atgctcttat gaccgatttt caacaatatt
     4681 ttaagccaat tgtttattct aagatagaaa tgtacttatg ctctttaaac tattgcatca
     4741 ataaatcttt cgactcattg atgcccgtta ctggcattcg cacgttgatt ttttcaatat
     4801 tatcactctg caaatgattt atcatctaac gtaattaaaa aacgaatcgt agaaaaatgc
     4861 gatcatagta ttccagcaac aattgcaaca tacgattgct ttctgttttg cctcagacaa
     4921 aagcacacaa ccccgtctca tcaacggggt tgtgtagtag aaaggttttg tgtgaagcga
     4981 tcagtagagt ttagccaagg cctccagctt attgagcaag agggtcgcta taccgggctg
     5041 attggacgcc gccgtaaagg atg
//
LOCUS       DAOQNQ010000232         5042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_6882_length_5042_cov_6.130940, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000232 DAOQNQ010000000
VERSION     DAOQNQ010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6882_length_5042_cov_6.130940"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="PK754_12285"
     CDS             <1..994
                     /locus_tag="PK754_12285"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB10329.1"
                     /translation="RTNSKRRAFITDNRVLLGLTGMDDEGKIKPLDVRQCGIEPLLHS
                     RIYAYVQQWLSSEASYMFARALNYVILRGDDRRITILLSVRDEDNRIKRDCTLLSKSL
                     SREFPMVVGFLLIRDEYSKYYMSDKAVSDPTALQKIFGERDVVQDVGEQQFSHSVLGF
                     SQTNPSVLPDFIAMVHDLISPQPHHTLYDLYCGYGLFGLSLAPNVEKVIGVEMSRHSV
                     DSARTNALRMKIANARFHIGDLSGLTLEPILKKQKTPFIAVLDPPRSGTAPGVIETLA
                     MAKPDRVVHVFCDIERLPYELKRWRQSGYRAAHVIPVDMFPATGNVEVAVLLET"
     gene            1071..1142
                     /locus_tag="PK754_12290"
     tRNA            1071..1142
                     /locus_tag="PK754_12290"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1104..1106,aa:Glu,seq:ttc)
     gene            complement(1317..2111)
                     /locus_tag="PK754_12295"
     CDS             complement(1317..2111)
                     /locus_tag="PK754_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNB10330.1"
                     /translation="MKSDNRYKQIIEKIFFNHFKNGMKEVPFERDEIERVAKNLKIKL
                     PKNIGDIIYTFRYRVALPDSIASKAPKGMEWIIRPAGQSKYKFVIAKFANIHPSEILS
                     QTKIPNATPGIIESYAFSDEQALLAKIRYNRLIDIFTGIACYSLQSHLRSNVSDLGQI
                     ETDEIYVGIDKKGVHYIIPVQAKGSADKLGIVQIEQDIALCKSKFPNLVAKPIGAQFI
                     TDDLIALFEFEISENGITVVSEKHYRLVNPENLSPHELLSYRNRTE"
     gene            complement(2114..3466)
                     /locus_tag="PK754_12300"
     CDS             complement(2114..3466)
                     /locus_tag="PK754_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNB10331.1"
                     /translation="MPSNQFAYNSFVEWDNQINGKRSPFSPLNNKLNLDDKRFHDWYR
                     FVLSYPAHLVRTYLQEFCSSQNDIVLDPFCGTGTTLVEAKLNGINSVGIEANPFAHFA
                     STTKINWEISADQFLKCALEIADLVRSEFEKSGIDDDDINMPEHTFPLRTLDSELEKI
                     ILTNSISPIPLHKTLVLLEKIKNHAQKKYYPHLLLGLAKATVFKTSNLKFGPEVGLGK
                     IKKNVPVIASWLFEINAIANDIRSIQIGKPKESVAHLGDSRQASALIKPQSITAVITS
                     PPYPNEKDYTRTTRLESVLLGFIRSKEELRQFKKTLIRSNTRGVYKDDDDHQWIANHP
                     EIKRIADQIEARRIELNKTSGFERLYSRVTLLYFGGMAKHLKELQPVLKPGARLAYVV
                     GDQASYLRIMIRTGEILADIARKLGYRVDRIDLFRTRFATATGEQLREEVVVLTWPGN
                     "
     gene            complement(3762..>5042)
                     /locus_tag="PK754_12305"
     CDS             complement(3762..>5042)
                     /locus_tag="PK754_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10332.1"
                     /translation="TTICATDQGLQIVGKGGRLVTAKNGLPSDKVKFIADDSQGILWL
                     ISDNKLVQFDKEPKMILYTGETNFTEVRGFFIDAKDNKWLATDRGLVRYDGTWKSYTQ
                     KDGLISDNVTCAAVSPSGKILTGTSSGVSYFNGTYWISYTTETGLPSNDVKAVLFTKD
                     ETVWVGTDKGIHIRKAEGDGDRVLLIRKVLAEEDSLLNIRAYDKLRTIYASVNIFGDL
                     TEWVAYKTANTYEMEKRWDDAFQLYTKIRTDNPVTTLATDARMYRVARAFENAGESAK
                     AMPIYFDLAERYKTYTDGNYRVEAALVRIIRNYQKENNIQRALEQARQIMAMYPRSPW
                     LREVRSIFTQITENAPPKETSTMALLQEWLNRFPDHSDVLRLKYKLASMYWEEQQFEY
                     AEKLYAEINSAAGTPKGLQSLLERRMAKRQLTKK"
BASE COUNT         1293 a         1078 c         1096 g         1575 t
ORIGIN      
        1 ccgtaccaat tctaaacgcc gcgcttttat tacggataat cgcgtgttgc tgggattgac
       61 cgggatggat gacgaaggaa aaatcaaacc tttggatgtg cgccaatgcg gtatcgaacc
      121 gcttttgcac agccgcattt acgcctacgt gcagcaatgg ttgtcctccg aagcttcgta
      181 tatgtttgcc cgtgcgttga attatgtcat tttgcgaggc gatgatcgcc ggatcaccat
      241 tctcctcagt gtgcgcgatg aagataatcg gatcaaacgt gattgcacct tgttatccaa
      301 atcgctgtcg cgtgaatttc cgatggttgt cggtttcctt ctgatcaggg atgaatattc
      361 aaaatattat atgtccgaca aagccgtgag cgatccgaca gcgttgcaaa aaatattcgg
      421 agaacgggat gtcgtccaag atgtggggga gcaacagttc tcgcattcgg ttttgggttt
      481 ttctcagacc aatccgtccg tacttccgga ttttatcgcg atggtacacg atctgatttc
      541 gccgcaaccg catcatacgt tgtacgattt atactgcggc tatggtttgt tcggtctgag
      601 tttggcgccc aatgttgaaa aagtaatcgg tgtagaaatg tcgcgtcatt cggtggattc
      661 agcgcgtacc aatgcgcttc gaatgaaaat tgccaacgca cggtttcata tcggcgacct
      721 gtccggtctt acgctggagc ccatactcaa aaagcagaaa acgccgttta ttgcggtatt
      781 ggatcccccg cgctcgggaa cggcaccggg tgttatcgag acgctggcta tggccaaacc
      841 ggatcgcgtc gtacatgtgt tttgcgacat cgaacgcctt ccgtatgaat taaaacgctg
      901 gcgtcaaagc ggctatcgcg cggcacatgt gatacccgta gatatgtttc cggcgacggg
      961 taacgtggaa gtcgccgtac ttttagagac gtgaaataaa taaaaaaaaa cttgcacaac
     1021 cgaaggtcgt cgcttatatt aggcgcactt ttgatgttct tagaaaatat ggccccatcg
     1081 tctaatggtt aggacatcgc cctttcacgg cggtaatagg ggttcgaatc cccttggggt
     1141 caccggccaa taaaaaaaac ctgcttacga taagcaggtt tttttgtttt aatcaaatgt
     1201 gaccgctatg atcaggagta aaatcttatt gctcctgata ttattttacc tcaataatag
     1261 gcaacacatt atatgttatg ccgattccta tgcgcattaa agttgtccgt acatcgctac
     1321 tcggtccgat tacggtagct tagtaattca tgaggggata aattttcagg attgaccaaa
     1381 cgatagtgtt tttcagaaac aaccgtaatt ccattttccg atatctcgaa ctcgaataac
     1441 gcgatcagat cgtcagtaat gaattgcgca ccaattggtt tggcgactaa gttaggaaat
     1501 ttgcttttac acaatgcgat atcttgttct atttgcacaa ttccaagttt atcagcagaa
     1561 ccttttgctt gcactggaat aatgtaatgc acccctttct tgtcaatgcc aacatagatt
     1621 tcatctgttt ctatttggcc gaggtccgaa acatttgatc gcaagtggct ttgtaatgaa
     1681 taacatgcta ttccggtaaa aatgtcgatt agtcggttat atctgatttt tgccaacagt
     1741 gcttgctcat cagagaaagc atatgactct ataataccag gtgtggcatt agggattttg
     1801 gtctgactca atatctcaga tggatggatg tttgcaaatt ttgcaattac aaatttgtac
     1861 ttagattgac cggcaggtct gattatccat tccattcctt taggtgcctt ggaggcaatc
     1921 gaatcaggca gtgcaactcg ataccgaaaa gtgtaaataa tatcgccgat gttcttaggt
     1981 agtttgatct ttagattttt tgcaacgcgt tcaatttcat ctctttcaaa tggtacttct
     2041 ttcatcccat ttttaaaatg gttaaagaat attttttcta tgatttgttt atatcggttg
     2101 tctgatttca tgattaattt cccggccatg tcaaaacaac tacctcttcg cgtaattgct
     2161 ctcccgttgc agtagcgaat cttgttctaa ataaatcaat ccgatcaact ctataaccta
     2221 actttcttgc aatatcagcg agaatttcgc cggttcggat cattatcctc aagtaagagg
     2281 cttgatctcc aacaacataa gctaatcgag cgcctggttt taataccggt tgtaattctt
     2341 ttagatgttt tgccattcct ccaaaatata ataatgtcac tctactgtat aaacgctcaa
     2401 atccagatgt cttattaagc tcaattcgac gcgcctcgat ttggtcggca atacgcttaa
     2461 tttcggggtg attggcaatc cattggtgat cgtcatcgtc tttgtaaacg cctctggtat
     2521 tcgatctaat caaagtcttt ttaaattgac gcaattcttc tttgcttcgt atgaagccca
     2581 acaaaacgga ttccaaacga gtggttcggg tgtaatcttt ttcatttgga tatggcggcg
     2641 aggtgataac cgcagtgatc gattgaggtt taatcaatgc actcgcctga cgggaatctc
     2701 ccaaatgcgc aacggattct ttaggtttgc cgatttggat tgatcggatg tcatttgcga
     2761 ttgcatttat ttcaaatagc catgatgcaa tgacaggtac attcttcttt atttttccaa
     2821 gacctacttc cgggccaaat tttaggttac ttgttttaaa cactgtggcc ttggctaacc
     2881 ctaatagtaa atgaggataa tatttctttt gggcatgatt cttaatcttt tctaacaaca
     2941 ctaacgtttt gtgtagtgga attgggctta tagaatttgt taaaatgatt ttttctaatt
     3001 cggaatcaag tgttcttaga ggaaatgtat gttctggcat attaatgtca tcatcatcaa
     3061 ttccactttt ttcaaattct gatctgacta gatcagctat ttcaagtgcg cattttaaaa
     3121 attgatcagc agatatttcc caattgattt tagtggtcga tgcaaaatga gcgaacggat
     3181 tagcttctat gcctacggag ttaattccat ttagctttgc ttctaccaga gtcgttccgg
     3241 taccgcaaaa aggatctagt acaatatcgt tttgactgct acaaaactct tggagatagg
     3301 ttcttaccag atgcgcggga taagacaata caaatcgata ccaatcatga aatcgtttat
     3361 catctaagtt gagcttgtta ttcagtgggc taaacgggct tcgcttaccg tttatttggt
     3421 tatcccactc aacaaacgag ttatacgcaa actgattcga tggcatcggt ttcctttata
     3481 gagtatatgt gacgcttgcc aagatagtta aaccgacttc gttgttccac agaaatttta
     3541 atgcatgtat tgaatgccga agcatcattt tccccttgat taagggcgca tagttccatc
     3601 gtagcattat aacagtcaca acgtagagta tatgaactat gtcgtttgaa attgctcaat
     3661 actttgaaaa aagttgtgta atagtgattt tgcgacttcc caccaataaa aaaacctgct
     3721 tatcgtaagc aggttttttt gttttatcgt gatccgtcgt tttatttttt ggtcagctga
     3781 cgtttggcca tacggcgctc caataaagat tgaagtccct tgggtgtgcc ggccgctgaa
     3841 ttgatttccg catataattt ttcggcgtat tcaaactgct gctcttccca atacatcgaa
     3901 gccaatttgt attttaaccg cagaacgtcg ctgtggtccg ggaagcggtt aagccattcc
     3961 tgcagaagcg ccatcgtgga cgtttctttg ggaggggcat tttcagtgat ctgagtgaat
     4021 atggatcgca cttcacgaag ccagggcgag cggggataca tggccattat ttgccgggcc
     4081 tgttccaacg cgcgctgaat gttgttttct ttttggtagt tgcggataat gcgaaccagc
     4141 gctgcttcga cgcgataatt gccgtcggtg tatgttttat aacgttcggc taggtcaaaa
     4201 taaatcggca tggctttggc cgattcgccc gcattttcaa aggcacgggc gacacggtac
     4261 atacgcgcat ccgttgccaa ggtagtgacc ggattatcgg tacggatttt tgtgtacaat
     4321 tggaatgcat catcccagcg tttttccatt tcatacgtat ttgccgtttt gtaggctacc
     4381 cattccgtca gatcgccgaa gatatttacc gatgcataga tggtacggag tttgtcatat
     4441 gcgcgtatat tcaaaagtga atcttcttcg gcaagtacct tgcgaatcag caatacacga
     4501 tcgccgtcac cttcggcctt gcgaatatgt atgcctttat cggttcctac ccacacggtc
     4561 tcatctttgg taaaaagtac ggctttgaca tcattggacg gtaatccggt ttccgtggta
     4621 taactgatcc aataggtacc attgaagtaa ctgacaccgg atgacgtacc ggtcaaaatt
     4681 ttaccggacg gcgaaacggc cgcgcaggtg acattgtcac tgataagtcc gtctttttgt
     4741 gtgtacgatt tccatgtgcc gtcatagcgt actaaaccgc gatcggtcgc cagccattta
     4801 ttgtccttgg catctataaa aaagccgcgc acctctgtaa aattggtttc gccggtgtat
     4861 aaaatcattt tgggttcttt gtcaaactgt accaacttgt tgtcggagat cagccacaaa
     4921 ataccctgac tgtcgtcggc gataaatttt actttatcgg acggcaaacc gtttttggcc
     4981 gttacaaggc gaccgccttt accgacgatc tgcaatccct gatcggtagc gcaaatcgta
     5041 gt
//
LOCUS       DAOQNQ010000233         5031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_6906_length_5031_cov_6.198151, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000233 DAOQNQ010000000
VERSION     DAOQNQ010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6906_length_5031_cov_6.198151"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2471
                     /locus_tag="PK754_12310"
     CDS             <1..2471
                     /locus_tag="PK754_12310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNB10333.1"
                     /translation="NLSGNKFTFEDIQPNVGIATFTYSGQDSLDSYASVSSAEASSRI
                     LVVTAGGTNNQYSWKKNNVAIPSTNNDTLYIDSVKTSDEGNYTCDVTNTVAIGTTLRR
                     KVITLTVTGTAPSTPTGVTASGVTTNRIDVTWNKPSGVITRYRVYRSTAAASGYAQVD
                     SVAAPTLTYSNTSLSSNTVYFYKISSVGNFGFSAQSSAASDTTLSVAPIVANAIPDTS
                     LFEGFGKTFRRKLTHVFSDADNTTLTYSVSTTPAELTATISHDTLYIASVAGFSGTAA
                     VVASAFDGYSTVTDTFNVTYILDVTAPTISNVTLPASVASGASLSVTATVTDNGSLSG
                     VKLFFQKGTSAYDSLTMTAGTNNTFTATVHDSNITTEGIALYIRARDSRVNTAISDTS
                     SVPVTFTTVASSIAGSKYPTGIPSNRWRLVSVPADLDSKNLSSILSGVYSGDYIAYNA
                     QPAEITALANGAGMWFYHKSPVDMPINTAAGKTNLTTGFDMSLAPGWNLIGNPYPFTI
                     NFAALEDTAFYGPIEYGVGATEAWSTEVTTLQPFGGYAIYVKGNAARTIRVTPDNFVW
                     MPKSSNQLEGEKFRIMLSAEGLKKQIRYGDLYTTLAVLDRDDAEAFAQPEPKNPGQTI
                     GVYFPSGDHGLNRKTIRFDKQGITSDFVLESGLESGELTMQIVNNEPGWSVRLFNVST
                     NSFIDHETAMSLPPSGTKWLMRILAGTPEYLAGKQTELEQLPKAFELLQNYPNPFNPG
                     TTIRYALASQAKVTLTVYNMLGQKVRTLVNGDRQETGLRTAVWDGRDERGALVSSGVY
                     LYRVELETTTGSRKSFTRKMLLVK"
     gene            2487..3722
                     /locus_tag="PK754_12315"
     CDS             2487..3722
                     /locus_tag="PK754_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10334.1"
                     /translation="MMRKNFYTMLMATLTTMSGLISCGTTRNSMLQDNPELIIALGGS
                     SYTQLSDADKYDAAEYLVRKGQYENARVIFEDIITHNPGVVAVKYKLAQLYLKLDSVT
                     FAEKTPKGDTYYFTKNGAELGEAVLREIYEKDPWFLPAYSELMMLEAKRQNYDGVKAI
                     YEKAITKHKDFSTSDYRVGFLTMGNPDLADRFSEGMRMMEKGKKTYTDLYESYKSLGN
                     IFNVQHKDTVAYNQLIRAISYRTEAIDIFSPYYHLADVCETLYQVKKEEHYKTMALQY
                     ACASLHYFPGYEPSVNVIRRLTGVAKDTSADSSKTYRAAAEAFCAGSTLPADDSVTIA
                     SGYTTVIPHSYLTEEMEAKQADIKAREIKRSRRPLLIAGGIAGGALAVLLLLSLVGGG
                     GGSSSGPLGGGPPDFPTPQ"
     gene            4047..4853
                     /locus_tag="PK754_12320"
     CDS             4047..4853
                     /locus_tag="PK754_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10335.1"
                     /translation="MISYLRSIGLSNPSQNAFLFDNQLPYRQSPAKVFCKIGRVVVAM
                     LMIGSNTIFAQLINMSGVVSSATDDAAIYTVSKGRFTNRISIGENCNANIVCNDERSL
                     PEHICARRSDGSGSVLVMSNRDAITSVAGQQTFPAMGEEIFGGVIIAWTDEWHDTFDQ
                     VIYTQCIEASCRFGISTVPASSASFEVITKIQEPLHWNEVSMACQYPVPDDMCCSQAF
                     TFHSSHFLEPLFYHATGTGICRSAPHQRSTYFLAFFHGRNVDIDFDIIKY"
BASE COUNT         1317 a         1127 c         1239 g         1348 t
ORIGIN      
        1 tcaatctttc gggtaataaa tttacttttg aagatattca acccaatgtc ggtatcgcca
       61 catttacata ctccggccag gacagtttgg atagttatgc atcggtcagc tcagcggagg
      121 cgtcttcgcg tattttagta gtcactgcag gtggcacaaa taatcaatat tcatggaaga
      181 aaaacaatgt cgctatacct tcaaccaata atgacacgtt gtacattgat tccgtgaaaa
      241 ccagcgatga gggaaactat acgtgcgatg tgaccaatac agttgcgata gggactacac
      301 tgcgccgaaa agtaataaca ctaaccgtga ccggtactgc gccatccaca ccgacagggg
      361 ttacggcctc cggtgtgacg accaaccgca ttgatgtgac ttggaataaa ccttcgggtg
      421 tcatcacacg ctaccgcgtg tatcgttcga cggcagcggc ttccggttat gcacaggtag
      481 attccgttgc ggcgcccaca ctgacttaca gcaataccag tctttcgtcc aataccgttt
      541 atttttataa aatctcgtct gtaggtaatt ttggttttag cgcgcaatcg tcggctgcaa
      601 gtgatacaac gcttagcgta gcgccgatcg tggcgaatgc cattcccgat acctcgcttt
      661 ttgaagggtt cggaaagacg tttcgccgga aactgacgca tgttttttcc gatgcggata
      721 atacaacatt gacgtattcg gtctccacga cgcctgcgga acttaccgca acgatttctc
      781 acgatacact ttatattgcc agtgtggccg ggttcagcgg tacagcggcc gtcgttgcaa
      841 gcgcatttga cgggtattcg acggtaacgg atacattcaa tgtgacttat attttggatg
      901 tgaccgcacc gacgattagc aatgttaccc ttccggcatc cgttgcatcg ggtgcatcgc
      961 tatcggtgac agcgacggtt acggataatg gcagtctatc gggggtaaaa ctatttttcc
     1021 aaaaaggcac atcggcttat gattcactga ccatgacagc cggtacgaat aatacattta
     1081 ccgctaccgt gcatgattcg aatatcacga cggaaggtat tgctttgtat attcgtgcgc
     1141 gggatagcag agttaataca gccatcagtg atacaagtag cgtaccggta acatttacga
     1201 ccgtggcctc atccatcgca ggatctaaat atcctacggg cattccatcc aatcgttggc
     1261 gtttggtgtc tgtaccggca gatctggata gcaaaaattt gagttcgatt ttaagcggtg
     1321 tgtattcggg tgactatatc gcgtacaacg cgcaacctgc agaaatcaca gccctggcca
     1381 atggtgcagg gatgtggttt tatcacaagt cgccggtaga tatgccgatt aatacggcag
     1441 cgggtaaaac caatctgacg acgggatttg atatgtcact ggccccggga tggaatctga
     1501 tcggaaaccc atacccgttc acgattaatt ttgccgcact tgaagatacg gcgttttacg
     1561 ggccgatcga atatggcgtc ggcgcgaccg aagcatggag cactgaagtc accacgctgc
     1621 agccttttgg cgggtatgcg atttatgtaa aaggtaatgc ggcgcgtacg attcgtgtaa
     1681 caccggataa ttttgtttgg atgcctaaat catcgaacca attagaaggt gaaaaatttc
     1741 gtatcatgct cagcgccgaa ggtttgaaaa aacaaattcg ctacggtgat ctttatacta
     1801 cgctggctgt attagatcgc gatgatgcgg aagcgtttgc acagccggag cctaagaacc
     1861 cgggacaaac aatcggcgtg tatttcccgt cgggcgatca tggtctcaat cgcaaaacga
     1921 tacgttttga taagcagggt attacctcgg actttgtatt ggaatccggt ttggagtcgg
     1981 gtgaactaac gatgcagatt gtcaataatg aaccgggttg gtccgtgcgt ttattcaatg
     2041 tcagcacgaa ttcatttatt gatcatgaaa cggcaatgtc cttaccgccg tcgggcacaa
     2101 aatggctgat gcgtattttg gcgggaactc cggagtatct tgcaggtaaa caaacggaat
     2161 tggaacaact tcctaaagcg tttgaattgc tgcaaaacta ccccaaccct ttcaatccag
     2221 ggacgacgat acgttatgca ctggcttcgc aggctaaagt gacgctgacg gtttataata
     2281 tgcttggaca aaaagtgcga acattggtca acggcgaccg ccaagagacc ggtttgcgta
     2341 cggcggtatg ggacgggcgt gacgaacgtg gcgctttagt ttcatccggt gtttatctat
     2401 atcgcgtgga attggaaaca acaaccggtt cgcgtaaatc attcacccga aaaatgcttt
     2461 tggttaaata aggatatagc aatcgtatga tgcgtaaaaa tttttacacg atgttgatgg
     2521 cgactctgac gacgatgtca gggttgattt cctgcggtac gacgcgcaat tccatgcttc
     2581 aggataatcc ggaactgatc attgcgctcg gcggatccag ttacacgcaa ctttccgatg
     2641 ccgataaata tgatgccgcg gagtatctgg tgcgcaaagg gcaatacgaa aacgcacggg
     2701 ttatttttga agatatcatt acgcataatc ccggtgtcgt ggccgtaaaa tataaattgg
     2761 cgcagttgta tctcaaattg gacagcgtga cgtttgcgga aaaaacgccc aaaggcgata
     2821 cgtattattt taccaaaaac ggcgcggaac tcggcgaagc cgtactccgt gaaatctatg
     2881 aaaaagatcc gtggtttttg cctgcgtatt ccgagctgat gatgcttgaa gccaagcgtc
     2941 aaaattatga tggggtcaaa gcgatttatg aaaaagccat aacgaaacat aaagattttt
     3001 ctacgtccga ttatcgcgtc ggatttctga cgatggggaa tccggatttg gctgatcgtt
     3061 tcagcgaagg tatgcgtatg atggaaaaag gaaaaaagac gtacaccgat ttgtatgaat
     3121 cctacaaaag ccttgggaat atctttaatg tacaacacaa agataccgta gcttacaatc
     3181 aactgatacg tgcgatcagc taccgtacgg aagcgataga catttttagc ccgtattacc
     3241 atttggccga tgtctgcgaa acgctgtatc aagtaaaaaa agaagagcat tataaaacga
     3301 tggcgcttca atacgcttgt gccagtcttc actatttccc cggatatgaa ccgtcggtca
     3361 acgtaattcg acgcttgaca ggtgtggcta aagatacttc cgccgattcc agtaaaacat
     3421 accgcgccgc tgcggaggca ttctgtgccg gcagtacatt gcctgcggat gacagtgtga
     3481 ctatcgcgtc gggatacacg acggtgatcc cgcacagtta tctgacggaa gaaatggaag
     3541 ccaaacaagc tgatatcaaa gcccgcgaaa tcaagcgttc ccgtcgtccg ctattaatag
     3601 ccggcggaat cgcaggcggt gcattggccg tgttactgct gctgagtttg gttggcggtg
     3661 gcggcggtag ttcgtccggt ccgctcggag gtggtccccc ggacttccca acgccgcagt
     3721 aaaaaagaag ttttgcgatc ccatattcat ggggtcggca aagggcttga cactgcgcag
     3781 cctttgcggt aatttggttc aaccgtttga ttatcttgtg gtaaaggtca cgactgataa
     3841 gatagagttt cggtattctc attcggacgt attttgagga aaacacgtcc tttacaatcc
     3901 catgagcggg cgctgttctc agcctgcaaa aacgaaattt gacgtttctt tcgatataca
     3961 cgcttcgtgc gttttgtata ttgcgttttt atagtctgcg cgattagaga aacggttata
     4021 taatatccac atctatggag atatccgtga tctcgtattt acgctcgatc ggtctttcca
     4081 acccatcgca aaacgcattt ctttttgaca accaattacc ttatcggcaa tcacccgcaa
     4141 aggtcttttg taagataggc cgtgttgtag ttgcgatgct catgatcgga agcaatacga
     4201 tttttgcaca actgataaac atgtcaggtg ttgtatcaag tgcgaccgat gacgcggcta
     4261 tctatacggt atcgaaaggc cgatttacca atagaattag catcggcgaa aattgcaacg
     4321 ctaatattgt ctgcaatgac gagagatcat tgccggagca catatgtgcc cgacggagtg
     4381 acggctcggg atcagttctg gtgatgagca atcgtgatgc aatcacttcg gttgcgggcc
     4441 agcaaacctt cccggccatg ggcgaggaaa tttttggcgg tgtgattatc gcatggacgg
     4501 atgaatggca cgatacattt gatcaggtta tttatacgca gtgtatcgaa gcatcatgtc
     4561 gttttggcat atccacggtt ccagcttcgt ccgcttcatt cgaggtgatt acgaaaattc
     4621 aagagccatt gcactggaat gaagtttcca tggcctgcca atatcccgtc cctgatgata
     4681 tgtgttgttc ccaagctttt acatttcatt cttctcattt tttggagcct ctgttttacc
     4741 acgcgaccgg aactgggatt tgccgcagtg cgccgcatca aaggagtaca tattttttag
     4801 cgttttttca cggtagaaat gttgacattg attttgacat aattaagtat tagctcgcat
     4861 attccattct ttcattatat cggagttcat ccatgaacgg attgccacga ctttatgaat
     4921 tggttttatc aattcgccat gatattccaa atctattccg tttccacgtt tcgaggatga
     4981 aacttgcacg cgtgttggtc aaaataacca tggccgtagc ggtcatgggt c
//
LOCUS       DAOQNQ010000234         4992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_7008_length_4992_cov_4.762609, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000234 DAOQNQ010000000
VERSION     DAOQNQ010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7008_length_4992_cov_4.762609"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PK754_12325"
     CDS             complement(<1..274)
                     /locus_tag="PK754_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNB10336.1"
                     /translation="MSATVTEKNVSSASTGMPEHHFLNSPQGLKSWLLTLDHKRIGVM
                     YLFGIMTMFFIGGVFALLVRLELMSPGEDIMGKDAYNKMFTLHGAVM"
     gene            complement(287..1216)
                     /gene="coxB"
                     /locus_tag="PK754_12330"
     CDS             complement(287..1216)
                     /gene="coxB"
                     /locus_tag="PK754_12330"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNB10337.1"
                     /translation="MNDFWMPPAHSTVAHEIDSLFYFIYYCSVFFFVAVVGAMTYFAF
                     KYKRKAHEMTEPAAHNNTLEITWTVIPTILCLVVFVLGFRGYMKMNVVPKDALEIKAT
                     GQKWFWSFDYPSGATTVNDIVIPVGKPVKLLMSSKDVIHSFYVAEFRTKRDVLPNRYS
                     ITWFEATQADTFLIQCTEYCGTSHSEMIGKVIAVSETDYNKWLEANSNPGEGLTPEAY
                     GEKLYTSKACITCHSLDGKPGSGPSWKAIFGKAEKMTDGSTVTIDENYLRESILNPQA
                     KIVAGYQPVMPTYQGILKDREIDAIIAYIKTLK"
     gene            complement(1256..2074)
                     /locus_tag="PK754_12335"
     CDS             complement(1256..2074)
                     /locus_tag="PK754_12335"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNB10338.1"
                     /translation="MRLRSLLCITVLLQYVCSTLDAQVILEKNQDPELQKIDVIEHLG
                     DTVPMNLQFKDEHGAIVQLSDYFEKGKPIVLTMAYYRCPMLCTMVLNAKAEAVKQLAW
                     LPGREFQMLTVSIDPNETPELASNKKDTYLKFLDKSGIQEGWHFLVGDSSQSKALADA
                     LGFKYFYDENRKEYAHPAVVFVITEKGIVSRYLYGIRFAERDMRLALMEASEGKIGST
                     IDRIIMYCFHYDPNAKGYVVMATNVMKLGGLVTLVVLGVFLTVMWRRDKTRSIQ"
     gene            complement(2097..2462)
                     /locus_tag="PK754_12340"
     CDS             complement(2097..2462)
                     /locus_tag="PK754_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10339.1"
                     /translation="MSENTQEQIHPEDGYEKSDVNARAILVSTLIGIILIVASLVLVD
                     EYFIQGREQFYTEAVLKPESAPLRDLRTKEAEFLNGYAVIDAKKGVYQIPITVAMERL
                     AAEAFEKTQTKKDGKISKK"
     gene            complement(2479..3672)
                     /locus_tag="PK754_12345"
     CDS             complement(2479..3672)
                     /locus_tag="PK754_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10340.1"
                     /translation="MKLNTETFRLEDAGSFGKKALMAGIVGLLISVAGYFVNADQFWR
                     SYLTAFTFWTSIGLGGMFFVLVKHLAGAEWSVVLRRLAENFMITLPIMFVFFLPILFG
                     SHHLYEWTHTEVVAKDPILSAKSGYLNMTFFTIRTVLYFAIWTIFGRMLYKMSIGQDG
                     GDQSKNNRMRQLSAPGMILFAFSITYASFDWIMSLQPHWFSTIFGVWYFAGSLLNFLA
                     MLVLIAIYLRKRGVLDNVISIDHFHDLGKLQFAFTIFWAYIAFSQFFLIWYANVPEET
                     IFFLKRWEGSWMYVSIGLLVCHLMIPFILLIPRANKRNLTIMKFVAIWFLFMHWVDLY
                     WNIFPTWHDAHHGVVMSGLWVDAATILGIGGIFLWYFWMLLGKQALAAHKDPSFEKSM
                     HHVNL"
     gene            complement(3677..4285)
                     /locus_tag="PK754_12350"
     CDS             complement(3677..4285)
                     /locus_tag="PK754_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNB10341.1"
                     /translation="MKSSKIWSTTVIVAVMALSACQGRPSEEPPIHLNPNMDSQPKYK
                     AQSKGDFFADGMSMRTPPEGTVARGQLREDAAYYMGRDEKGNLLKENPVPVTMALLHR
                     GQERFNIYCTPCHGQTGLGNGIVVQRGMLPPPNFHDERLLKVEDGHFFDVMTNGIRNM
                     SSYKSQVPVADRWAITAYIRALQRTHTASLEDVPQEKRKDLK"
     gene            complement(4311..4862)
                     /locus_tag="PK754_12355"
     CDS             complement(4311..4862)
                     /locus_tag="PK754_12355"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HNB10342.1"
                     /translation="MSASKEKQTDEVAIVLAEFDGPATLIEAAKKMRDAGYKAFDCHS
                     PFPVHGMDQAMGLARSPLAKICFVIAMSAVLGMAYFTYWLSFDYKLVISGKPLFSYVA
                     YVPPVYAVGILTCALTAVVGMMVLNGLPQPYHPLFNSDNFARVTQDGFFVSVESTDPQ
                     YDEAKVKSLLASLGGTNVEVVKG"
BASE COUNT         1412 a         1229 c         1060 g         1291 t
ORIGIN      
        1 ccatcacggc gccgtgcagt gtaaacattt tattgtaagc gtctttgccc atgatgtctt
       61 cgcccggact catcagttcg aggcgtacaa gcaatgcgaa tacaccgccg ataaagaaca
      121 tcgtcatgat accaaaaagg tacatcactc cgatgcgctt gtgatccagc gtcaacaacc
      181 aagacttcaa cccttgcggg ctgtttaaaa agtgatgctc aggcatccct gtggacgccg
      241 aactcacatt tttttccgtc actgtcgcag acatattaat taccttctat ttaagagttt
      301 tgatataagc gatgatagcg tcaatctcac ggtctttcag aatgccttga tacgtcggca
      361 tcaccggttg ataaccggcc acgattttgg cttgcgggtt tagtatggat tcacgcaaat
      421 aattttcatc aatcgttaca gtagaaccat cggtcatttt ttcagcttta ccaaagatcg
      481 ctttccaact gggacctgaa ccgggtttac cgtccaagct atggcatgta atacacgctt
      541 tggatgtata tagtttttca ccgtaggctt caggggtcaa accttctccc ggattgctat
      601 tggcttcgag ccatttattg taatctgttt cacttaccgc aatcactttt ccgatcattt
      661 ccgaatggct tgtaccgcaa tattccgtac actgaatcaa aaacgtatcg gcttgtgtcg
      721 cttcaaacca tgtgatggag tagcggttgg gcaatacgtc gcgcttggta cgaaattctg
      781 caacgtaaaa actatggatt acatctttgg aggacatcag aagtttgacc ggttttccga
      841 ccgggatgac aatatcgttt actgtcgtag cgccggaagg gtaatcaaaa gaccaaaacc
      901 atttttgccc cgtcgccttg atttcaagcg catctttggg aacgacattc atcttcatgt
      961 agccgcgaaa acccaacacg aaaacaacga gacacaaaat cgtcggaatc accgtccacg
     1021 tgatttccaa cgtattatta tgcgctgcag gttcggtcat ttcatgcgct ttacgtttgt
     1081 atttaaacgc aaagtaagtc atcgcgccga cgacagccac aaagaaaaag acagaacagt
     1141 aataaatgaa atagaagaga ctgtcaatct catgcgcaac cgtcgaatgc gcgggtggca
     1201 tccaaaaatc attcatacga tcgttttcct ttaattatag aatttatatt aaaatttatt
     1261 gaatacttcg tgttttatcg cgacgccaca tcaccgttaa aaaaacaccc agcacaacca
     1321 gcgtgaccaa tccgcctaac ttcatcacat ttgtagccat gactacataa cctttggcat
     1381 tcggatcata atggaagcag tacataatga tgcggtcaat cgtacttcct atttttcctt
     1441 cggacgcctc catcagagcc aatcgcatat cgcgttcagc aaatcgtatc ccgtacaaat
     1501 aacgggaaac gatgcctttt tcggtgatca caaagactac cgccggatgc gcatattctt
     1561 tccgattttc atcgtaaaaa tatttgaacc ccaatgcatc cgccaacgct ttggactgcg
     1621 acgaatcgcc gaccaaaaaa tgccagccct cctgaatacc ggacttatca agaaacttca
     1681 ggtaggtgtc ttttttattc gacgccagtt ccggtgtttc attgggatct atgctaacag
     1741 tcagcatttg aaattcacgc ccgggtaacc atgctaattg tttgacggcc tcggctttcg
     1801 cgttgagcac catcgtacat aacatcggac agcgataata agccatcgta agaacgatcg
     1861 gtttaccttt ttcgaagtag tccgacaatt gtactatcgc tccgtgctca tctttaaatt
     1921 gcaaattcat cgggacggta tcccccagat gttcgatcac atctattttt tgcaactcgg
     1981 gatcctgatt cttctccaga atcacctgcg cgtccagcgt agaacataca tattgtaaaa
     2041 gaacagttat acaaagtagc gatcgtaatc gcatgaaaat tctttcagat cagactttat
     2101 tttttggaaa ttttaccgtc ttttttggtc tgcgttttct cgaaagcttc cgcggccaaa
     2161 cgttccatcg cgacggttat cggtatttga taaacgcctt ttttggcatc aatcactgca
     2221 tatccgttga gaaattccgc ttctttggta cgcaagtcac gcagaggggc cgattccggt
     2281 ttgagaaccg cttcagtata aaactgttca cgtccttgaa taaaatattc atcaaccagc
     2341 accagcgacg cgacgatcaa aatgatacca atcaatgtag ataccagaat agcacgtgca
     2401 ttgacatcgg atttctcata gccgtcttcg ggatggattt gttcttgtgt attttcactc
     2461 atcgcccgta tcctcagatt aaagattaac gtgatgcata gatttttcga aactcggatc
     2521 tttgtgagcg gctaatgctt gtttgcccag cagcatccaa aagtaccaaa ggaatatacc
     2581 gccgataccc aaaattgtcg ccgcgtccac ccataaaccg gacattacta caccgtgatg
     2641 ggcatcatgc cacgtcggaa aaatattcca atacagatct acccagtgca taaataaaaa
     2701 ccaaatcgca acaaacttca taatcgtaag attgcgttta ttcgcacgcg gaatgagtaa
     2761 aataaacgga atcatcaagt ggcaaacaag taatccgata ctgacataca tccaggagcc
     2821 ttcccagcgc ttgaggaaga aaatcgtttc ttcaggtaca ttggcatacc aaatcaggaa
     2881 aaactgagaa aatgcgatat aagcccagaa aatagtaaaa gcgaattgaa gttttccgag
     2941 atcgtggaag tgatctatcg aaatcacatt atcaagcaca ccgcgtttgc gcagataaat
     3001 ggcgatcaat accagcatgg caagaaaatt gagcaagctg ccggcaaaat accacacgcc
     3061 aaaaatcgtt gaaaaccaat gcggttgcaa tgacatgatc cagtcaaatg atgcatacgt
     3121 gatcgagaag gcaaacaaaa tcataccggg cgcactgagt tggcgcatgc ggttattttt
     3181 tgattgatcg ccgccatcct gaccgattga catcttgtag agcatacggc caaaaatcgt
     3241 ccatattgca aaatacagca ccgtacggat ggtaaaaaac gtcatattca aataaccgga
     3301 tttggcgctc agaatcgggt ctttagcgac cacttcggta tgtgtccatt catacaaatg
     3361 atggctgccg aataaaatgg gaagaaaaaa tacaaacatg atcggcaaag tgatcatgaa
     3421 gttttcggcc agacgtctta aaaccacact ccactccgca ccggccaaat gcttgaccaa
     3481 tacgaagaac ataccgccaa gaccgatgga tgtccagaaa gtaaaagccg tgaggtaaga
     3541 acgccagaat tgatcggcat tcacaaaata tccggcaacg ctgatcaaaa gaccgacaat
     3601 accggccata agcgcttttt taccgaatga accggcgtct tccagacgaa aggtttcagt
     3661 attcagtttc ataagtttac ttcaagtcct tacgtttttc ttgtggaaca tcttccagag
     3721 acgccgtatg cgtacgctgg agggcgcgga tataggccgt gatagcccag cgatccgcta
     3781 ccggtacttg gcttttgtag gaagacatgt tacggatacc gttggtcatc acatcgaaaa
     3841 aatgaccgtc ttcgactttg agcaaccgtt catcgtgaaa attaggcggc ggcaacatac
     3901 cccgctgtac gacgatcccg ttgcccaatc cggtctggcc gtggcacggt gtacaataaa
     3961 tattaaatcg ttcctgaccg cgatgaagca aggccatggt caccggcacc gggttttctt
     4021 tgagaagatt tcctttttcg tcgcgaccca tgtagtacgc cgcgtcttca cgcagttgcc
     4081 cgcgagcgac cgttccttcc ggcggcgtac gcatagacat gccgtcggca aaaaaatcac
     4141 ctttagactg cgccttgtat ttaggctgac tgtccatgtt ggggttcaag tgaataggcg
     4201 gttcttccga gggacgaccc tggcaagcac tcagcgccat caccgctaca atcaccgtcg
     4261 tggaccaaat ttttgaagat ttcatagaca ctatagttaa gatgtttgaa ttatcctttg
     4321 acaacttcca cattggtgcc gccgagcgac gccagaaggg atttgacttt ggcctcgtca
     4381 tactgcggat cggtggattc cacgcttaca aagaatccat cttgcgtaac gcgggcaaaa
     4441 ttatccgaat taaacagcgg atggtacggt tgcggaagac cgttgagcac catcatgccg
     4501 accaccgccg tcaaagcgca tgtcaaaatg ccgaccgcat ataccggcgg gacgtacgca
     4561 acatagctga ataacggttt gccggaaata accaatttgt aatcaaaact gagccaatat
     4621 gtaaagtacg ccataccaag cacagcgctc atcgcaatga cgaaacatat tttagccaaa
     4681 ggggaacgcg ctaaacccat cgcctgatcc atgccgtgca ccgggaacgg tgaatggcag
     4741 tcaaacgctt tatatccggc gtcccgcatt tttttggcgg cttcgatcaa tgtcgccggg
     4801 ccgtcaaatt cggccaaaac gattgcgact tcatctgttt gtttttcttt tgatgcactc
     4861 atattcgtat cccgattaat gttttccgtt gtgatgatgc ggatccgcct gcggtagtac
     4921 gcccttgatt tcggccatgg caatcatcgg cacaaagcgc agggtattac ccagaatcgg
     4981 caggaaatcc tt
//
LOCUS       DAOQNQ010000235         4991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_7011_length_4991_cov_5.978525, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000235 DAOQNQ010000000
VERSION     DAOQNQ010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7011_length_4991_cov_5.978525"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="PK754_12360"
     CDS             complement(<1..1206)
                     /locus_tag="PK754_12360"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNB10343.1"
                     /translation="MNQLVLRLLMLSVVLLGACAPKPPRVEEWLTYYEKSNHLETPRY
                     DETVAYLQRLEQASPWVNLTYFGVSPQGRMNPLVIVDKDRRFNPQAARQSGKNVLLIQ
                     AGIHAGEIDGKDAGMMLIRDLVIFKKNPELLDHTVILFMPFFNVDGHERFGPYNRINQ
                     NGPKEMGWRTTAQNLNLNRDYMKADAPEMRDWLKLFQAWKPDILLDCHVTDGADYRHA
                     VTYAIDQSQNLAPSVRSFTKDVYMPELKNAMAQSGFPMSPYVNLVEERDIAQGLLGGN
                     SPPRFSTGYAALQNRIGFLIETHMFKDYKTRVDGTYNVILHTLETMHRHAPELRQTML
                     DGDASAERMTGSYLPITFRWSKADTIDFLGYNFRMEKSAVTGESFVYWENVPIDYRLP
                     FYDQVSTTDS"
     gene            complement(1215..2018)
                     /locus_tag="PK754_12365"
     CDS             complement(1215..2018)
                     /locus_tag="PK754_12365"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNB10344.1"
                     /translation="MNTEPHIESHLIVETPENIFLEFELAGLGSRFLAYLIDTAMQAI
                     FFIILVYVLAIAFAFIGGLLHIEEISDGWRNALLAIGIFLLYEGYFIFFETVWRGQTI
                     GKKILRLRVVKDEGYPITFMDAAIRNLMRIADALPTWFYFPSYGLGSAVLIGNPQGKR
                     LGDFAAGTLVIKERTTTGFENFGLLHLNPEMVRGITLANLNRINDDEYFVLRSFFYRK
                     NAFEPMRRMALAKTISAHFRTKLNISQDAWPNDIRLLDTMMLLIEEKKR"
     gene            complement(2028..2669)
                     /locus_tag="PK754_12370"
     CDS             complement(2028..2669)
                     /locus_tag="PK754_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNB10345.1"
                     /translation="MHHSTFSPDREFLVTDEDTLKKLYGTPSEASLAKETDYMHPHYR
                     RFIEVSPFFVLATSGPHGLDASPRGDKPGFVTVENEKTLLIPDRRGNNRMDSLRNIIH
                     NPSVALFFLVPGIGESLRVNGRAQITVDPAWRSRFEMENKLPATVLIVHVEKVFFQCA
                     RAIQRSGLWDPSLHITRDQLPSTGTILEDLSAQRITASEYDRDLYVRQKNTLY"
     gene            complement(2712..3536)
                     /gene="mazG"
                     /locus_tag="PK754_12375"
     CDS             complement(2712..3536)
                     /gene="mazG"
                     /locus_tag="PK754_12375"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013868848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNB10346.1"
                     /translation="MESSHETEKIVLDDDASTEAVRTQFGRLYNIMRELRAKCPWDKE
                     QTHASLRQYLLEEAYETLEALDENNYTELRKEMGDLMLQILFHSRIASENNHFDLVDV
                     LRTINDKLIRRHPHVFGDIEAKTADKVLENWEQIKLKEAGRTSVLDGVPKVLPALVRA
                     YRVQEKASRVGFDWGHVDEVWKKVHEEVKELEEAARSGQLQKMEDELGDVLFSVVNLA
                     RFMPINPEDALRGTIEKFTRRFQYVERKYVENQKDMKTATLEEMDQYWDEAKRLGY"
     gene            complement(3545..>4991)
                     /locus_tag="PK754_12380"
     CDS             complement(3545..>4991)
                     /locus_tag="PK754_12380"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB10347.1"
                     /translation="WQKKFGKTVYRETATAWNLIGNVAVAERDTAGAREYFEKATNTN
                     PYDIMARKNLARLYEFDRRWDKAVSMYGQVLTLESQDITARLSLANLMYQKKRYVDAA
                     RYYVSAVMIKPESENQRENPIQILQWMSANRKDIKPLQTLMDSVDERILDDATDPKKQ
                     FELRITSGYVYLYYLQNPTEAIHEFKSAQSLNSRAYRLDYYLGEAYFQVGSYDLALPF
                     LRRYAEHAEDSYTYAKVFLLLAKTLNKLKRYEEAQIEIIKSIRVYPNAESYYVQGIAF
                     RGTQNNEEAVRAFERAVKVFPNYTQAHYELGRAHMALRKYDKALPELQKAVELDSSNY
                     PYRQTLARWYSTLERWEEADASLAATERRMLSAGVKDPTVYEDWGEQYFQQKKYDVAA
                     QKYTLAYGADTTTIQACFRLASIAASQHDGTSALRWLSRAFRNRFVDFNALLKDKNFN
                     ALRQTPEYVELVGAYERAYNEEVQKKINLTR"
BASE COUNT         1222 a         1179 c         1239 g         1351 t
ORIGIN      
        1 ggaatcggta gttgatacct gatcataaaa aggtaagcgg taatcaatgg gcacgttttc
       61 ccaatacaca aacgattcgc ccgtcacggc acttttttcc atgcggaaat tgtagccgag
      121 aaaatcaatc gtatccgctt tggaccagcg gaaagtaatc ggtaaataag atccggtcat
      181 gcgctccgcc gaagcatctc catccaacat ggtctgtctg agttcgggtg catgccgatg
      241 catcgtctca agagtgtgca ggatgacatt gtatgtgccg tccacgcggg ttttgtaatc
      301 tttgaacata tgcgtttcga tcaaaaaacc gatacggttt tgcagagcgg cataaccggt
      361 cgaaaatcgc ggaggggaat tgccaccgag caaaccttga gcgatatccc gctcttcgac
      421 gaggttgaca tacggtgaca tcggaaaacc ggattgcgcc atggcgtttt tcaattcagg
      481 catatagacg tcttttgtaa acgaccgcac tgacggagcg aggttttgcg actgatcaat
      541 agcgtacgtc accgcatgcc gataatcagc gccgtcggtg acatggcaat ccaataagat
      601 atcgggtttc cacgcctgaa acagtttgag ccagtcgcgc atttcgggcg catcggcttt
      661 catgtaatcg cggttgaggt tcagattttg cgcggtggta cgccaaccca tttctttggg
      721 gccgttctga ttgattcggt tgtacggacc aaaacgctcg tgcccgtcaa cattgaaaaa
      781 aggcataaat aaaatcacgg tatgatccag cagttccgga tttttcttaa aaatcacgag
      841 atcacgaatc agcatcattc cggcatcttt accgtctatc tcaccggcat gaatgccggc
      901 ttgaatcaaa agtacatttt ttccgctttg gcgggccgcc tgaggattga aacggcgatc
      961 tttatccacg atgacaagcg ggttcatacg gccttgcggg ctgacgccga agtacgtcag
     1021 attgacccaa ggcgaggcct gctcaaggcg ctgaaggtaa gcaacggttt cgtcgtaacg
     1081 cggggtttcg aggtgattgg atttttcgta atacgtcagc cattcttcca cgcgcggcgg
     1141 cttgggtgca catgcgccaa gaagtactac cgataacata agaagtcgca ggacaagttg
     1201 attcatgatt tcctctatcg ttttttttct tcgattaata acatcatcgt atccaaaagc
     1261 cggatatcgt tgggccaggc atcttgggaa atatttagtt ttgtacgaaa atgcgccgaa
     1321 attgttttgg cgagcgccat tcgacgcatc ggttcgaatg cgttttttct gtaaaaaaag
     1381 gagcgtagca caaaatactc atcatcgttg atccggttta aattggccag cgtgataccc
     1441 cgaaccattt cgggattgag atgcagcaac ccgaaatttt caaatccggt cgttgtacgt
     1501 tctttgatca ccaatgttcc ggcggcaaaa tcgccaagcc gtttaccttg cggattaccg
     1561 atcaaaaccg cgctgccgag cccgtaactg ggaaaataaa accatgtggg caaagcatcg
     1621 gcgatgcgca tcagattgcg aatagccgca tccatgaaag tgatcgggta cccttcgtct
     1681 ttgacgacgc ggaggcggag gatttttttg ccgatcgtct gtccgcgcca gacggtttca
     1741 aaaaaaataa aatatccttc ataaagcaag aaaataccga tggccaaaag tgcattacgc
     1801 catccatcgg aaatttcttc gatatgtaac aagccgccga taaacgcgaa ggctatggct
     1861 aaaacataaa ccaaaataat aaaaaaaatc gcctgcatgg ccgtatcaat caaatacgct
     1921 aaaaaacgag agccaaggcc ggccaattcg aactccaaaa aaatattttc gggtgtttcg
     1981 acgatcaaat gcgattcgat gtgcggttcc gtattcatgc ttcgatatta atacaacgta
     2041 tttttctggc gaacatacaa gtcccggtca tactccgagg cggtgatgcg ctgcgcactc
     2101 aagtcttcca aaatagtacc cgtgctggga agctgatcgc gggtgatatg caaagacgga
     2161 tcccacaggc cggaacgctg gatggcacgt gcacattgga aaaatacttt ttcgacgtga
     2221 acgattaaaa cggtcgccgg aagtttgttt tccatttcaa atcgtgatcg ccatgccgga
     2281 tcgacggtaa tctgtgcgcg gccgttgaca cgaagcgatt cgccgatacc cggtaccaga
     2341 aaaaaaagcg ctacggaagg attgtgaatg atattacgca ggctgtccat gcgattattg
     2401 ccgcggcgat ccggtatcaa aagtgttttt tcattttcga cggtcacaaa acccggctta
     2461 tccccgcgcg gtgacgcatc gagcccgtgc gggccggatg tcgctaacac aaaaaaaggc
     2521 gacacttcga taaatcggcg ataatgcgga tgcatgtaat cggtttcttt agcgagcgat
     2581 gcttcgctgg gtgtgccgta taattttttt agtgtgtcct cgtcggtaac aaggaattcg
     2641 cggtcgggag aaaaagtaga atgatgcatg cgttgataaa atttcgtgtt ttttagaatt
     2701 gaaggctcat ctcagtagcc gagtcgtttc gcttcatccc aatattggtc catttcttcg
     2761 agcgtagcag ttttcatatc cttttgattt tcgacatact tccgttcgac atattgaaaa
     2821 cggcgggtaa atttttcgat cgtaccgcgg agcgcgtctt cgggattgat cggcataaaa
     2881 cgtgcgagat tgacgacgga aaaaagtacg tccccgagct catcttccat tttttgaagt
     2941 tgtccggatc gtgcggcttc ttctaattct tttacttctt cgtgtacttt tttccatact
     3001 tcgtccacgt gaccccagtc aaaaccgaca cgcgaggctt tttcctgtac gcgatacgcc
     3061 cgtaccagtg cgggtaaaac tttgggtact ccatccaata ccgaggtgcg accggcttct
     3121 ttgagtttga tctgttccca attctccagc actttatcgg cggttttagc ttcgatgtca
     3181 ccgaatacat gcggatgccg gcggatcagt ttatcattga tggttcgcaa tacatccacc
     3241 agatcaaaat ggttgttttc ggaggcaatg cgcgaatgaa aaagaatttg cagcatgagg
     3301 tcgcccattt ctttgcgcag ctcggtataa ttattttcat ctaaggcttc gagcgtctcg
     3361 taggcttctt ccaaaagata ctgtcgcagt gaggcgtgcg tttgttcttt atcccaaggg
     3421 catttggcac gcaactcacg catgatattg tataagcgac caaactgcgt acgtacggct
     3481 tctgtcgaag cgtcatcatc caatactatt ttttcggttt cgtgtgaaga ttccaccgct
     3541 tctcctatcg ggtcaaattt atttttttct gtacttcttc gttgtaagcg cgttcatagg
     3601 cgccgactaa ttcgacatat tcgggcgtct gacgcagggc gttaaagttt ttatccttga
     3661 gcaacgcgtt aaagtccaca aaacgatttc tgaaagcgcg actcagccat cgcagggcgg
     3721 aagtgccgtc atgctgggat gcagcgatac tggccaatcg aaaacaagcc tggatcgtcg
     3781 tcgtatcggc gccgtaggcg agagtatatt tttgagcggc cacatcgtat ttcttttgct
     3841 gaaaatactg ttcgccccaa tcttcataca ccgtggggtc tttgacaccg gcgcttaaca
     3901 ttcgccgctc cgtcgccgcc agtgacgcat cggcctcttc ccatcgctcg agcgtgctgt
     3961 accagcgtgc gagagtctgg cggtacggat agttggacga atccagttcg accgcttttt
     4021 gtagttccgg gagcgctttg tcgtatttgc gaagcgccat gtgggcgcgg cctaattcgt
     4081 aatgcgcctg tgtatagttg ggaaatactt tgacggcccg ttcaaatgcg cgaacggctt
     4141 cttcgttatt ttgtgtaccg cgaaacgcga taccttgaac gtaatacgat tcggcattgg
     4201 ggtacacgcg aatgcttttg atgatttcga tttgcgcttc ttcgtaccgc ttgagcttgt
     4261 taagcgtttt agcgagcagc aaaaatactt tggcgtaggt atagctgtct tcggcatgct
     4321 cggcataacg acgcaggaaa ggtaatgcaa gatcatacga gccgacctga aaataggctt
     4381 caccgaggta gtagtccaac cggtatgcgc gcgaattcaa cgattgcgcg gatttgaatt
     4441 catggatggc ttcggtgggg ttttgcaaat aatacaaata tacataaccg gaagtgatgc
     4501 gcagttcgaa ctgctttttg gggtcggtgg cgtcgtcaag aatgcgttca tctacggaat
     4561 ccatcaatgt ttgcagaggt ttgatgtctt tgcggttggc ggacatccac tgtaggattt
     4621 gtatcgggtt ttcacgttgg ttttccgatt cgggttttat catcacggcg ctcacataat
     4681 accgtgcggc gtcaacatag cgttttttct gatacatgag attggccaaa gataatcgcg
     4741 cggtgatgtc ttgcgattcc agagtcaata cttgtccata catggatacg gctttgtccc
     4801 agcgacggtc aaattcgtat aaacgcgcca ggtttttgcg cgccatgata tcatacggat
     4861 tggtgttcgt tgctttttcg aaatattcgc gcgcaccggc tgtatcacgt tcggcgaccg
     4921 cgacattgcc gatcagattc catgccgtag cggtttcccg gtaaacagtt ttaccgaatt
     4981 ttttctgcca t
//
LOCUS       DAOQNQ010000236         4974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_7064_length_4974_cov_8.438097, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000236 DAOQNQ010000000
VERSION     DAOQNQ010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7064_length_4974_cov_8.438097"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PK754_12385"
     CDS             <1..335
                     /locus_tag="PK754_12385"
                     /inference="COORDINATES: protein motif:HMM:NF016148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HNB10348.1"
                     /translation="KLQSEVLPIYQKSRARTIATTTTTTSMNAGSHAMMQRFKDDIEY
                     QQWFSQKDDKVRESFNYDHVAADGQRVQIGQPFIVSGEALRYPGDPSGSLGNIINCRC
                     FAIPIKKK"
     gene            642..1673
                     /locus_tag="PK754_12390"
     CDS             642..1673
                     /locus_tag="PK754_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10349.1"
                     /translation="MIDEIYNKQEQYFREHLNNEDRRGARSFGFSRMADDKASDGEPV
                     FIVNTPSVDGHRSVISPRGLDFSNKYYKNNPIWVFDHDNRLVVGQADGSGWIKYSKAD
                     KSEIKSKIRYASTDFPQEVRTLVVENILRMASIWFDPIVQFFDQAARSEYEKDYADLG
                     LKAPDYMNWYIKESGLLHNSIVAIGSNLDAYAQARGSYSPEMQRAIEFAIMQEVIPSL
                     RADIAALRAELVTLRDNGAKNLTESIKEKEQAIADKVADMETERKAGDGESPLCQSDK
                     AVSIPDPQKVIEATIARDVNDARKLSVDENTMKEFNAWKRKRAELDSLRTSIDKTFKT
                     IKYYQGQVE"
     gene            1673..2959
                     /locus_tag="PK754_12395"
     CDS             1673..2959
                     /locus_tag="PK754_12395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HNB10350.1"
                     /translation="MEDKKVEVPEADLDAFRKFQAEQAEAARKEKEKNELSDIQVLRD
                     KQDAMQMMLEKALKYNELVASNRSISKLAIDNVKDEKETRAKKWDVLMRAFLAGDLPK
                     YNEVAKELSSVTRGFVTTNSTAQYNVPSDVRTDLLYTTETFGILGRDTMNYTTQSNSV
                     QLPFLNTSLSVGFVAQAAAPISQSGTATQVTLSIAAMKAILGTFSEETLDDAPSLYNS
                     WVQILGKGIMQKRDDTLFNGDGTAAYGLFKGIFQEVAATGGITEKSIAGGALSTITID
                     DLRGIIAAVPVSARAGAKFYFHRTFEPFLYGMKDLNNRPIFIEGNTANPSTLLGYPVE
                     LSEVCPDVSGVVGLGTKFLAFGNLKNSCHFVDRQQMTMKIGYEGTVDGEAMFAEGKSA
                     LRVMERWALKVYTMPQPFLGAASNVRRLGLVTLATA"
     gene            2986..3156
                     /locus_tag="PK754_12400"
     CDS             2986..3156
                     /locus_tag="PK754_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10351.1"
                     /translation="MDYKGKSYNQGEKVDIEEIHVHHALKNELVEKYEPSDTTRMNVT
                     ENTTRPKKGKMK"
     gene            3153..3623
                     /locus_tag="PK754_12405"
     CDS             3153..3623
                     /locus_tag="PK754_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10352.1"
                     /translation="MKKILILMFVLCADLFAQANTIPIVWARFSAPNTQITTISGTDS
                     LFSNSFGGQIDSLHLVCGLPANDSLRVKITLQSGINNVWENVAVDFDSVVSVGTQQLK
                     RVDLKFRAGNLTNASMRLKIRAYNTAAVTTANGVSTGAPDLSSVYWKVYTRLRP"
     gene            3707..4363
                     /locus_tag="PK754_12410"
     CDS             3707..4363
                     /locus_tag="PK754_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10353.1"
                     /translation="MALSSYAIFDDVSDAYGVLGISGDAPESQKSFIEDCINAATEDL
                     EQLLNRNLISRAYTDESHTIESELQYDREGREYNIAPTILYLRHYPITAITSIKFDNV
                     ALTDITEGETTGIWWSASDLANSGRLVYESGWPCGERRIKINYTAGYTVSNLPYIYRR
                     VGNELIQWHYSKSGYGDKSLQMAQIQGTISPGTGNFRTHDEMMTYFGKQLTPYRSING
                     "
     gene            4356..>4974
                     /locus_tag="PK754_12415"
     CDS             4356..>4974
                     /locus_tag="PK754_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10354.1"
                     /translation="MAKITTNSDKLSRDFERVKAETVKMYAALAQNVFDQMAIDSTGN
                     IRIFGGTTPYLAKVARRAAMVKPPISNKLTSRSGRLVASLNNKTKAGQIEAIRKIEMN
                     GSGVTLTYGTSTPYALIHDKGGTINVSAHQRTSKFSATPIRSDLSTRDTIFKYRFAGN
                     NKRVTSSKNFNKKNNIVSLTYKVKGYSINMPARPYVSKALLELPKK"
BASE COUNT         1517 a          952 c         1214 g         1291 t
ORIGIN      
        1 tgaagttgca aagtgaggta ttgccgatat atcaaaaatc acgcgcaagg actatagcaa
       61 ccaccacaac gaccacatcc atgaacgctg gaagccatgc gatgatgcaa agatttaaag
      121 atgacataga atatcagcaa tggttttcac agaaagatga caaggtcagg gagagcttta
      181 actatgacca tgtagcggcg gacggtcagc gcgtgcagat agggcaaccg ttcatcgtga
      241 gcggcgaagc tttgcgctat cccggcgacc cgtctggcag cttaggtaac ataataaact
      301 gtaggtgctt tgctataccg ataaagaaaa agtaaaaaac acttgacatt taaaatttaa
      361 ttttgtataa tggccaacgt gaggttgtat gatttattcc ctgagcttga gaacgacatt
      421 gtcaagctaa ttttagacgg taagtacgcg gaatttaaaa taaagtttga agcgggtaag
      481 cccgtaataa gtgaaacgac ggagaaaaaa aggtacacgg aaaagaagca gtaattaaat
      541 agtttaagct tatctaaaca atagagggct ttttgtctta gttgcgccgc acaggggtgg
      601 cgtgattaga cgaaaagccc ttttttattt ggtggaataa gatgattgac gagatttaca
      661 ataaacaaga gcagtatttt agagaacact tgaacaatga ggaccgccgc ggggcgcgtt
      721 ctttcgggtt ttcgcgtatg gcagatgata aagccagtga cggggagcct gtgtttattg
      781 taaacacgcc gtctgttgac gggcatagat cggtgatttc tccgcgcggc ttagactttt
      841 ctaacaaata ttacaaaaac aatccgatat gggtgttcga tcatgacaat cgcttggttg
      901 tagggcaagc tgacggcagt ggatggataa aatattctaa agccgataag tctgaaatta
      961 aatccaagat acgttatgcg tcaaccgatt ttcctcaaga ggtgcgcacg cttgttgttg
     1021 agaatatttt gcgtatggct tcaatatggt ttgatccaat agttcagttt tttgatcaag
     1081 ccgctcgttc tgaatatgaa aaagattatg ccgacttagg attaaaagca cctgattaca
     1141 tgaactggta cataaaggaa tcgggtctat tgcataatag tattgtagcg ataggttcaa
     1201 atttagatgc atacgcgcag gcgcggggaa gttattcgcc tgaaatgcaa cgggctatag
     1261 agttcgcaat tatgcaagag gtcatcccat cattgcgagc cgatatagct gcattgcgtg
     1321 ccgaacttgt cacattgcgt gacaatgggg ctaagaattt aacggaatca ataaaagaaa
     1381 aagagcaagc gatagcggat aaggtggcgg atatggagac agaacgcaag gcgggagacg
     1441 gtgaaagccc gttatgccaa agcgataaag ctgttagtat tcctgaccct caaaaggtta
     1501 tcgaagcaac aatagcgcgt gacgtaaatg atgcgcgtaa attatcggta gatgagaaca
     1561 cgatgaaaga gtttaatgcg tggaaacgaa aacgcgcgga gcttgattcg ttgagaacgt
     1621 ctattgataa aacattcaaa acaattaagt attatcaagg acaggtggaa taatggaaga
     1681 caaaaaagta gaagtgccgg aagccgattt agatgcgttc cgtaaatttc aggcagaaca
     1741 ggcggaagcg gcgcgtaaag aaaaagaaaa aaacgagtta tccgatattc aggtattgcg
     1801 cgataagcaa gacgcgatgc agatgatgtt ggaaaaagct ttgaaataca atgaacttgt
     1861 ggcgagcaac cgaagcataa gcaagcttgc gattgacaat gtaaaagatg aaaaagagac
     1921 ccgcgctaaa aaatgggatg tacttatgcg ggcatttttg gctggcgatt tgccgaaata
     1981 caacgaggta gcaaaggaat tgtcatccgt aacgcgcgga tttgttacaa ccaatagcac
     2041 cgcgcaatat aatgtgccgt ctgacgtgcg caccgatttg ttatacacga cggaaacttt
     2101 cggtattctt ggccgagaca cgatgaatta tacaacgcaa agcaattcgg ttcagttgcc
     2161 ttttttgaat acttcgctat cggtcgggtt cgtcgcccaa gccgccgcgc ctatatctca
     2221 aagcggtacg gctactcagg ttacgttaag cattgccgca atgaaagcaa ttcttggtac
     2281 atttagcgaa gaaacgcttg acgacgcacc gtctttatac aatagctggg tgcaaattct
     2341 cggtaaaggt atcatgcaaa aacgcgatga taccttgttt aatggcgatg gaacggcggc
     2401 ttatggtttg ttcaagggta ttttccaaga ggttgccgct accggcggta tcaccgaaaa
     2461 atccatcgcc ggcggtgcgc tttcgacaat tacgattgac gacttgcgcg gaataatcgc
     2521 cgccgttcct gtttcggcac gcgctggggc taaattctat tttcaccgca cgttcgagcc
     2581 gttcttgtac gggatgaaag atttgaataa tcgcccgata tttatagagg gcaataccgc
     2641 gaatccctct acgttattgg gttacccagt cgaacttagt gaagtatgtc ccgatgtttc
     2701 gggtgtcgtg ggtctcggaa ctaaattcct tgctttcggt aatcttaaaa attcttgcca
     2761 tttcgttgat cgccaacaga tgaccatgaa gatcggatat gaaggcacgg tggacggcga
     2821 ggctatgttt gccgaaggta aatcggcgtt gcgtgtgatg gaacgctggg ctttaaaggt
     2881 ctatacaatg ccccaaccgt tcttgggcgc ggcaagtaat gtgcgccgct tgggtcttgt
     2941 aactttggct acagcgtaat gttgtgcata gttaaatacc ctcgcttgga ctacaaaggc
     3001 aaaagctata atcaaggcga aaaggtggac attgaagaaa ttcatgtcca ccatgccttg
     3061 aaaaacgagc ttgtagagaa gtatgagccg agcgatacaa caagaatgaa tgtaacagaa
     3121 aatacaacga gaccaaaaaa aggaaaaatg aaatgaaaaa aatattgatt ttaatgtttg
     3181 tactttgcgc tgatttattt gcgcaggcga atacgatacc tattgtatgg gcgcggtttt
     3241 ctgctccaaa cacgcaaatt actactatca gcggcacgga ttctttgttc tcaaattcgt
     3301 ttggcgggca aattgattct ttgcatttag tatgcggatt gccggcaaat gattctttgc
     3361 gtgttaaaat taccttgcag tccggaataa ataatgtttg ggaaaatgtt gccgttgact
     3421 ttgattccgt ggtatcggta gggacgcaac agcttaagcg cgttgattta aagtttcgcg
     3481 ctggtaatct aacgaatgct tcgatgcgtc taaaaattcg cgcatataat actgcggcgg
     3541 taactacggc aaacggcgtt tccaccggtg cgcctgattt gtcaagcgtt tattggaaag
     3601 tctatacacg tcttcgccct taattttcta cgcggttatt taacgctgta gaacatggtt
     3661 aaaggggctt gaaaaatagc cccttttata aagtgggatt aaaaatttgg cactatcaag
     3721 ctatgcaata tttgatgatg taagcgatgc ctatggcgtt ttgggcatat cgggcgacgc
     3781 gccggaatcc caaaaatcgt ttatcgaaga ttgcataaac gcggctacag aggatttaga
     3841 gcagctttta aaccgcaatc ttatttcacg cgcatacacc gacgaatcgc ataccataga
     3901 atcggaattg caatatgata gggaagggcg ggagtataat atcgcgccta ccattttata
     3961 tttgcgtcat tatccgataa ccgcaatcac atctatcaag tttgacaacg tagcacttac
     4021 cgatattacg gagggagaaa caactgggat ttggtggagc gcatcggatt tggctaatag
     4081 cggtcgtttg gtttatgaaa gcggttggcc gtgcggtgaa cgtaggataa aaataaatta
     4141 cacggcgggt tataccgtat cgaatctgcc ttatatttat cgccgcgtag ggaatgaatt
     4201 gattcaatgg cattattcca aatcgggcta tggtgataag tcgctacaaa tggcgcaaat
     4261 acaaggcact atttcgcccg gtacggggaa tttcagaaca cacgatgaga tgatgacgta
     4321 cttcggcaaa caacttacgc cctataggtc tatcaatggc taagataacc acaaattcgg
     4381 ataaactatc gcgtgatttt gagcgcgtaa aggcggagac ggtgaagatg tacgccgcgc
     4441 ttgcgcaaaa cgtgttcgat caaatggcta ttgatagcac gggtaatata agaattttcg
     4501 gcggcactac tccttattta gctaaggtgg cacgccgtgc tgcgatggta aaaccgccaa
     4561 tatctaataa gttaacatca agaagcggac gacttgttgc ttctttaaat aataaaacta
     4621 aagctgggca aattgaagct attagaaaaa ttgaaatgaa tggctccggc gtcactttga
     4681 cttatggtac atcaacgcct tacgctttga tacatgataa gggcggcact ataaatgtat
     4741 cagcgcatca aagaacatca aaatttagcg caacaccgat acgatccgat ttatctacac
     4801 gcgatactat ttttaaatat aggtttgcag gcaacaataa gagagttacg agctctaaaa
     4861 attttaacaa gaaaaacaac attgtttctt tgacatataa agttaaggga tactccataa
     4921 atatgccggc gcgtccttac gtatctaagg cgttgttaga attgcctaag aaaa
//
LOCUS       DAOQNQ010000237         4945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_7148_length_4945_cov_3.440286, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000237 DAOQNQ010000000
VERSION     DAOQNQ010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7148_length_4945_cov_3.440286"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2562
                     /locus_tag="PK754_12420"
     CDS             <1..2562
                     /locus_tag="PK754_12420"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HNB10355.1"
                     /translation="GLEAKAGTISSALSRFMAWKTADGKKEASHLVSQMITLIDGMLN
                     KETLLDLIRYFTVFEKSKHEDLHTKITTIESVKKIAAYHQYYAVQKAVASTIGASSDK
                     GTRKGGVVWHTQGSGKSLSMVFYTSKMVLALDNPTIIIITDRNDLDDQLFDTFAASTQ
                     LLRQVPVQADSRENLKSLLKVASGGIVFTTVQKFFPQDDELKFPLLSERKNIVVIADE
                     AHRTQYGFEAKTKERKDKEGNTLGTEITYGFAKHMRDALPNATFIGFTGTPVELTDRN
                     TKTIFGDYIDVYDISQAVLDGATVRIYYESRLVKVHLEEGEKEKLDDRLLMVAEGAPE
                     YLVDNAKSKWTRIEAIVGQTDRMKEVAKDIVKHFEDRQQVFIGKAMIVAMSRRIAVLL
                     YDQIIKLRPDWHHQDDDKGAIKVIMTGSSSDPASYQPHVRDKDRRKAIGERLKDDKDP
                     LKMVIVRDMWLTGFDAPCLHTLYVDKPMRGHSLMQAIARVNRVFNEKPGGLIVDYIGI
                     ASDLKKALAVYTESGGKGEPALDIDMAVRAMNEKLEVVRQMMHGYDYKTFYTAPLAEK
                     LAIILTAEEHILSLESGKERFIREVTLLSKAYALCKSTAEADTVSAEVSFFQAVKARL
                     AKFDTYGGGEGNEAIETVIRQLVDKAVAADGVIDIFDAAGIKKPDISILSDEFMKEIQ
                     GMKFKNLAIELLRKILSDEIRTRSKYNIIQSKALSEMLENTIRKYQNNLLTAAEIISE
                     LIDLAKNVKDADKRGEKLNLSVEELAFYDALEVNDSAVQVLGDEILRQIARILVERVR
                     ANTSIDWQIKENVRAKLRVIVRRVLREYGYPPDKQEKAVDTVLAQAETLAEIWAA"
     gene            3094..3777
                     /locus_tag="PK754_12425"
     CDS             3094..3777
                     /locus_tag="PK754_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HNB10356.1"
                     /translation="MKFQDFERIMTTARLGRYQAACGGNSKRAMTLYRKNLQLSQELF
                     TVISCFEIALRNAIDKQLLQTRGNDWLRNGATAGGIFDNPNCRLTKENIDDAMHKLNH
                     FYTHNKLVAELGFGFWRYMFAPNQFAATGKTLLQIFPAKPTSTAAIQYNHTFVFNQLA
                     QINNMRNRIAHHEPICFVPGQSVKGTTYARQHYGLILQLFQWMSIDEGKLLYGIDHIN
                     TVCNQIDAL"
     gene            4082..4807
                     /locus_tag="PK754_12430"
     CDS             4082..4807
                     /locus_tag="PK754_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HNB10357.1"
                     /translation="MKTTDYILFTINRLPRGYLFTYADFKKEVGQKEAIIKALNRMAA
                     SGKIAKLSKGKYYKPESTPFGNLQPNQAQVVKDLLEENGKVTGYLTGYSIYNRLGLTT
                     QVSNVIQIGKNQVRARFKRELYTIAFIKQKNTITKENVPLLQLLDAIRYIKKIPDANI
                     EASCKRFLAIIKNFTDKEINTLVRLALKYPPATRALLGALLEQLRKGNTAESLFNSLN
                     PITHYKLIGAAKVLPTAEKWNIK"
BASE COUNT         1546 a         1070 c         1150 g         1179 t
ORIGIN      
        1 ggactggaag cgaaagcagg gacgatctct tccgcattgt cacgcttcat ggcctggaaa
       61 acggccgacg gcaagaagga agcctcgcat cttgtcagcc agatgatcac gctcattgac
      121 ggcatgctga ataaagagac tttgctggat ctcatccgct atttcaccgt ttttgaaaag
      181 tcaaaacacg aagaccttca taccaagatt acgacgatag agtcggtcaa aaagatcgcc
      241 gcgtatcatc agtattacgc ggttcagaaa gccgttgcgt ccaccatcgg cgcttcctct
      301 gataaaggca cgcggaaagg cggcgtcgta tggcacacac agggttccgg gaaatcgctg
      361 tcgatggtgt tttacacgag caaaatggtt ctcgcgctgg ataatcccac gatcattatt
      421 atcaccgacc gaaacgatct cgacgaccag ctttttgaca ccttcgcggc atcaactcag
      481 cttctgaggc aggttccggt gcaagccgat agccgggaga acctaaaatc actgttgaaa
      541 gtcgcgtcgg gaggcattgt atttaccacc gtgcagaagt ttttcccgca ggacgacgaa
      601 ttgaaattcc cgttattatc cgaaagaaaa aatatcgtcg tcatagccga cgaagcgcac
      661 cgcacgcagt acgggtttga agccaagacc aaagaaagaa aagacaaaga aggtaatacg
      721 ctcggaacgg agatcacgta cggttttgcc aaacacatgc gcgacgcttt gcccaatgcg
      781 acgtttatcg gatttaccgg tacgccggtg gaactcaccg accggaatac gaaaaccatt
      841 ttcggtgatt atatcgacgt gtacgatatt tcccaagccg ttctcgacgg cgcgacggtt
      901 cgcatatact atgaaagccg gttggtaaaa gttcatctcg aagaaggcga gaaagaaaaa
      961 ctcgacgacc ggcttttgat ggtggccgaa ggcgcgccgg aatatctcgt tgacaacgca
     1021 aaatccaaat ggacgcgtat cgaagctatt gttggccaga ccgaccgtat gaaagaagtc
     1081 gccaaagata ttgtaaaaca ttttgaagat cgtcagcagg tatttatcgg caaggcaatg
     1141 atcgtggcga tgagccgccg tattgcggtg cttctgtatg accaaattat caaattgcgc
     1201 cccgactggc atcatcagga cgacgacaaa ggcgcgatca aagtgatcat gaccggttcc
     1261 tcatccgatc cggcgagtta ccagccgcat gttcgcgata aggatagacg caaagccatc
     1321 ggcgagcgat tgaaagatga taaggacccg ttgaagatgg tgatcgttcg cgatatgtgg
     1381 ctcacaggat ttgatgcgcc gtgcctgcac acgctgtatg tggacaaacc catgcgcggg
     1441 cacagtctca tgcaggcgat tgctcgggtg aaccgcgtat tcaatgaaaa acccggcgga
     1501 ttgatcgtgg actatatcgg cattgcttcg gatttgaaaa aagcgctagc agtctatacc
     1561 gaaagcggcg gcaagggcga accggcgctg gatattgaca tggccgttcg tgcgatgaac
     1621 gaaaaattag aagttgtccg gcagatgatg cacggatatg attataaaac gttttatacc
     1681 gcgcccttgg ctgaaaaatt ggcgatcatt ctgaccgctg aagagcatat actatctctc
     1741 gagagcggta aggagcgttt tattcgtgaa gtaacgctgc tgtccaaagc gtatgcgcta
     1801 tgcaaatcta cggcggaagc cgatacggtg tcggcggaag tttcattctt ccaggcggtg
     1861 aaagcgcggc tggcgaagtt tgacacctac ggcggcggag agggcaatga agctattgag
     1921 accgtgattc gccaattagt tgataaggcg gtcgcagcag acggagtaat cgacatattt
     1981 gatgcggccg gaatcaagaa gccggatatt tcgattctgt ccgatgaatt catgaaagaa
     2041 attcagggta tgaaatttaa gaaccttgcg atcgaattgc tccgcaagat tctgagcgat
     2101 gaaattcgta cacgatcgaa gtacaacatt atccagagca aggcgttatc ggagatgcta
     2161 gaaaatacga ttcgcaaata tcagaataat ctgctcaccg ccgctgaaat tatcagcgaa
     2221 ttaattgatt tagcgaagaa tgtcaaagat gcagacaagc gaggcgagaa actcaatttg
     2281 tccgttgaag aactggcgtt ttacgatgcc ctggaagtta acgacagcgc ggtgcaggtt
     2341 ctaggtgacg agatcttgcg ccagattgcg cgcatacttg ttgaacgagt tcgcgccaat
     2401 acgtcgatag actggcagat caaagaaaat gttcgcgcaa aattgcgcgt gatagttcgc
     2461 cgcgtcttac gcgaatacgg ttatccgccg gacaaacagg aaaaggccgt tgatacggtt
     2521 cttgcgcagg ccgagacttt ggcagagata tgggcggcgt gaatgttggg atcataagga
     2581 atatcaataa ctacgttccc ttctcccctt acaacaaaaa tttgccgcgc tggttgagaa
     2641 agttgaaccc atgcacgcca agcagcgcgc gtcggagcag gaattggatt tgctttttca
     2701 agcattaatg caaagagcat ttaaaggaga attggtaagt tgacttgccc tgagcgatgc
     2761 gccttcggcg cagagtaaag aggcatagat tgtttaagga agacaagcac agcgtgcaag
     2821 attggggctt tgtgattcac gttcggagat tcagcactta ttgattatgt cagtttacag
     2881 gatgctctgt aatccttgag aacacaaacc cacaaaccat tggtggcaat atcggagacc
     2941 actatgaact ttgagcagat tactgaaaat tatagactta gaaaccctca taaaaaacga
     3001 aacccgatag tttgaattgc ttcagggact accgggaata agacatcatt aacatacata
     3061 catttgaacg ataaaagcaa ataaaagttt acaatgaaat ttcaggactt tgaaagaata
     3121 atgacaaccg ccagattggg tcgctatcaa gcggcttgcg gtggtaatag caaaagagcc
     3181 atgacgcttt accgaaagaa cctgcaactt tcacaggagc tttttactgt catcagttgc
     3241 tttgagattg ctttgcgaaa cgccatagac aagcagcttc ttcaaacacg cggaaatgat
     3301 tggttgcgaa acggcgcaac cgcaggcgga atttttgaca atcccaattg ccgattaaca
     3361 aaagaaaaca ttgacgatgc aatgcataag ttgaatcatt tttacacaca caacaaactt
     3421 gtggctgaat tgggttttgg attttggcgt tacatgtttg ctccaaacca atttgctgcg
     3481 accggcaaaa cgcttcttca aatattcccg gctaagccaa caagcacagc agctattcag
     3541 tataatcata catttgtatt taatcagtta gctcaaataa acaatatgcg aaaccgcatc
     3601 gctcatcacg aacctatttg ttttgttcca ggacaatctg taaaaggaac aacttatgca
     3661 cggcaacact acggactgat tttacaatta tttcaatgga tgagtatcga cgagggaaaa
     3721 ttactttacg gaattgacca cattaacaca gtttgtaatc aaatagatgc cttataaaac
     3781 aagatgcaac ttgtagagaa agaaaccctg ccaccaaaac gggtttgggg cgaaatgcta
     3841 aaatcaagtg cagtttttca atttaaattt agcgcttatt gatcccgcaa gtttgtggga
     3901 tgcgccgaaa tctcccactg cgccaagccg tgtccgttaa ccaaggtgtt aaagaaaaat
     3961 agagtatggt aacttcacaa gaccgttgcg aggttacaat catttggttt aatcttaaaa
     4021 tgtccagttt ttttaataat aaactggaca aagtaattac tttttattat aatcatgcat
     4081 aatgaaaaca actgattata tattatttac aattaataga ctcccaagag gatacctatt
     4141 cacctatgcc gattttaaaa aagaggtggg tcaaaaagaa gcaatcataa aagccctgaa
     4201 ccgtatggcg gcttcgggca aaattgccaa actttccaaa ggaaaatatt acaagcccga
     4261 aagcacccca tttggcaatc ttcagcccaa tcaggcacaa gtagtaaaag atttgttgga
     4321 agaaaatgga aaagtaaccg ggtatctcac cggctatagc atttataacc gattgggcct
     4381 aaccacacag gtaagcaacg tcatacagat tggtaaaaat caagttcgcg cccgttttaa
     4441 aagagaactc tatactattg cttttataaa gcaaaaaaac accatcacca aagaaaatgt
     4501 cccattgttg cagttactgg atgctatccg gtacatcaaa aaaataccgg atgccaatat
     4561 agaagcatcc tgtaaacgat ttttggcaat catcaaaaac tttacggaca aagaaatcaa
     4621 taccctggtt cggttagcat taaaatatcc acccgctaca agggccctat taggtgcatt
     4681 attggaacaa ttgcgaaaag gcaacacggc tgaatcactt ttcaattcat tgaacccgat
     4741 tactcattat aaactaatcg gtgcagcgaa agtgttaccc actgctgaaa aatggaatat
     4801 caaatgaagt tgcaccacga catgaaattc acagaagaag aatcaaaaag gacatttacc
     4861 gagtttttgg ggcattacga acctgacaca caatcaataa taaaaaaatg agaagcaccg
     4921 gctataacac aagttttgca tcaaa
//
LOCUS       DAOQNQ010000238         4922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_7199_length_4922_cov_5.525991, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000238 DAOQNQ010000000
VERSION     DAOQNQ010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7199_length_4922_cov_5.525991"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1515)
                     /locus_tag="PK754_12435"
     CDS             complement(<1..1515)
                     /locus_tag="PK754_12435"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNB10358.1"
                     /translation="MQVNPHTEIKFLKGVGPKRAEALSGISIHTVWDLLHYYPRRYLD
                     RSKITSIRQARIHEEVTIIGQVENFALIRNHRGKGSRFRLILSDPTGSISLVWFRGAQ
                     YFEKAFEVGETLAVYGKIDYYNREIQIAHPEIDRLDADENVNFMHTGSIIPLYASSES
                     LKRMGFDSRGFRRLIFPVLDCASLVEDTLPASLLQNLSLAPLDKTLRHIHYPSTHEEL
                     ADAVRRIKFEELFYLQLMMAFRKSQITHAQIGIPFVNPGEKAKMLAEKLPFKLTDEQR
                     KVLKEIYADMRSDKPMHRLIQGDVGSGKTVVALLAIVSAVESGYQAAFMAPTEILAEQ
                     HYYVIQDYLWGMGIQAALIKGGQKKSERNKIIEDIRTRQVDIIVGTHAIFQEHVEFAK
                     LGLVVIDEQHRFGVMQRAEIRAKALDKGIIPDVLVMTATPIPRTLAMAMYGDLDISVI
                     GELPTGRKPIKTAIRREDARKKIYEFIRQEIRNGRQCYIVYPLIEESEKLDLKAA"
     gene            1610..2014
                     /locus_tag="PK754_12440"
     CDS             1610..2014
                     /locus_tag="PK754_12440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB10359.1"
                     /translation="MMMSTEVSEIKKQHTVLIVDDEDEVRDMLRRILKLSSYRVLEAQ
                     NAQEAMEIAVREKPDVITMDIMMPDKDGITLCEEIKANPQTKHIPVVMVTVVGQRQRA
                     MTAGADYFMNKFFTIDELVSVIDKLIADKQTA"
     gene            complement(2021..3160)
                     /locus_tag="PK754_12445"
     CDS             complement(2021..3160)
                     /locus_tag="PK754_12445"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HNB10360.1"
                     /translation="MLYLPDDYIQRLGDFLQLHLDEPIPRTLKPFAEAITEISEGLKK
                     KNKRSEFIRRIYLDQKVFRDAYLLYYGSINFPKMHIPLAEIQKSDFFNQKKNLRVLDA
                     GSGTGSMLLGLAYWLKNETTLTAELTASDYASSSLDTLSAFYKALALPYPLRTQILNL
                     NHAVPSENKFDLITAGNVLNELEPDAESKILPWLESALTQDGFVIWIEPALMDLSRRL
                     LIFRDRLLKEGWFLYSPCFTNKPCPALTEETDWCHHDVPWQRPEFIRVLDGMTGHIRQ
                     SLKFTYIVMGKRDIHLSDVTIGNRDYLSTVRVVSERFEEKGRIRVFGCNDRGRCAYSM
                     NKRDKSDDNLAFKKIERYDVVQLTETEEKSNYVGIGKNTKVRIVE"
     gene            complement(3234..4508)
                     /gene="serS"
                     /locus_tag="PK754_12450"
     CDS             complement(3234..4508)
                     /gene="serS"
                     /locus_tag="PK754_12450"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNB10361.1"
                     /translation="MLDLKFIRDHTERVKTAAKEKKFEADVDAVIRWDDERKVVIRKV
                     EDLKARRNKASEEVAKLKKAGQNADAVIAETKSVGEEIKALDEQLKVIEKNLRDVLLT
                     IPNVPHPSAPQGTSPEDNQTIFTWGTMPSADFELKPHWELAEKHNLIDFATGVKIAGA
                     GFPVYRGAGAKLQRALINFFLDSAADNGYTEVQPPIVVNTDSATGTGQLPDKEDQMYV
                     VTRDEFFLIPTAEVPVTNIHREMTLKAEQLPIKYAAYTPCFRREAGSYGKDVRGLNRL
                     HQFDKVELVKFVHPDTSYDELESLRMDAERLLQALQLPYRTLLMCRGDMGFTQTKKYD
                     LEVWAAGQKRWLEVSSCSNFESFQARRMNIRYKDNQTGKPEFVHTLNGSGLALPRIVA
                     AIMENYQTDEGTIMVPQVLRKYMGMDIISATT"
     gene            4710..>4922
                     /locus_tag="PK754_12455"
     CDS             4710..>4922
                     /locus_tag="PK754_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10362.1"
                     /translation="MYKTMSVLIVMGLLWAACRPSALPPLPKVYDPHSNARAEQAYVT
                     HMALDVRVDFDNKQISGRATFDVVVSD"
BASE COUNT         1266 a         1147 c         1153 g         1356 t
ORIGIN      
        1 ggccgccttc aaatctaatt tttctgattc ttcgatgagc ggatagacaa tataacattg
       61 tcgcccgttg cggatttcct ggcgtataaa ttcataaatt ttttttcgcg catcttcgcg
      121 acgaatcgcc gttttgatgg gtttgcgtcc ggtgggcagt tcaccgatca cggaaatgtc
      181 cagatcgccg tacattgcca tcgccaaagt gcggggaatc ggtgtggccg tcatgaccag
      241 cacatcgggg ataatgcctt tatccaaagc tttggcacga atttccgctc gttgcatgac
      301 accgaaacgg tgttgctcat caatcacgac caatcctaat ttggcaaact ccacatgttc
      361 ctgaaaaatc gcatgcgtac cgacgatgat atcgacctga cgcgttcgga tgtcttcgat
      421 gattttattg cgctccgatt ttttctgtcc gcctttgatc agcgccgcct ggatacccat
      481 accccataga taatcctgaa tgacatagta gtgttgctcg gcgagaattt ccgtcggcgc
      541 cataaaagcg gcctgataac cgctttcgac ggccgatacg atggccaata acgccacgac
      601 ggttttacca ctgccgacgt caccttggat caatcggtgc atcggtttgt cggagcgcat
      661 atcggcgtaa atttccttga gcactttgcg ctgttcgtcg gtgagtttaa aaggtaattt
      721 ttcggcgagc attttggctt tttcgccggg attaacaaaa ggaatgccaa tttgcgcatg
      781 ggtgatctgg cttttgcgga aagccatcat gagttgaagg taaaataatt cctcaaattt
      841 tatacgacgg acagcgtcag caagctcttc gtgcgtggaa ggataatgaa tatggcgtaa
      901 tgttttatcc agcggggcaa gggataaatt ttgaaggagc gatgccggta acgtgtcttc
      961 cacaagggat gcacagtcca gtacgggaaa aatcaatcgc cgaaaaccgc ggctgtcaaa
     1021 acccatacgt ttgagtgatt cggatgaggc gtacagcggg ataatcgagc ctgtatgcat
     1081 gaagttaaca ttttcatcgg cgtctaagcg atcaatttcc ggatgcgcga tttgtatttc
     1141 ccgattgtaa tagtcaatct tgccgtacac cgctaatgtt tcgccgactt caaatgcttt
     1201 ttcgaaatat tgtgctccgc gaaaccatac caagctgatg gagccggtcg gatcgctaag
     1261 aataagccga aaacgcgaac ctttaccgcg gtgattgcga atcagggcaa aattttccac
     1321 ttgtccgatg atggtcactt cctcgtgaat gcgcgcctga cgtatactgg tgattttgct
     1381 gcggtccaga tacctgcgcg gataataatg gagtaagtcc catacggtat gtatactgat
     1441 accggacaat gcttccgcac gtttggggcc gacgcctttg agaaatttta tttcagtatg
     1501 gggatttacc tgcatgcgta taacggtatt atgatcgtat attttcgatg aaacacattg
     1561 cttacgaggc cgcggcttcg tattttaacc cgtaaattga aagaaaatca tgatgatgtc
     1621 aaccgaagtc tccgaaatca aaaaacagca tacggttttg atcgttgacg atgaagacga
     1681 agtgcgcgat atgttacggc gcatattaaa attatcatcg taccgggtgc tcgaagcaca
     1741 gaatgcgcag gaagctatgg aaatagctgt ccgtgaaaaa ccggatgtca tcacgatgga
     1801 tattatgatg cccgataagg acggcatcac gctttgcgaa gaaatcaaag ccaatccgca
     1861 aaccaaacat ataccggttg tcatggtgac ggtagtcggc cagcgtcagc gcgcaatgac
     1921 tgcaggcgcc gattatttta tgaataagtt tttcaccatt gatgaactgg tatccgtgat
     1981 tgataaactc atcgcggata aacaaaccgc ttaaaacctt ctattccacg atacgaactt
     2041 ttgtattttt tccgataccg acatagtttg atttttcctc tgtctctgtc agttgcacca
     2101 cgtcataacg ttcaattttc ttaaatgcca aattgtcgtc agatttatca cgtttgttca
     2161 tactgtacgc acaacgtccg cggtcattgc acccgaaaac ccgtatcctg cctttttctt
     2221 caaaccgttc gctgacaacc ctcacggttg aaagataatc acgatttccg atcgtcacat
     2281 cgctcagatg gatatcgcgt ttgcccatga cgatatatgt aaacttgagt gactgacgaa
     2341 tgtgacccgt catgccatcc agtacacgaa taaattcagg ccgttgccac ggtacatcat
     2401 ggtgacacca gtcggtttcc tccgtaagcg cagggcaagg tttgttcgta aaacaagggc
     2461 tgtataaaaa ccaaccttcc tttagcaggc ggtcacgaaa tatcagaagt cgccttgata
     2521 aatccatcag cgccggttcg atccaaatta cgaaaccatc ctgagtcaag gccgattcaa
     2581 gccaaggaag aatcttcgat tcggcatcgg gttccaactc attgaggaca ttgcctgcgg
     2641 tgatcagatc aaatttattt tcggagggta cagcgtgatt aagatttaag atttgcgtac
     2701 gaagcgggta gggcagcgcg agggctttgt aaaacgcgga taatgtatcc agtgaagaag
     2761 aagcgtaatc cgacgccgtc agctcggccg tcagcgttgt ttcgtttttc agccaatagg
     2821 ccagtcccaa taacatgctg ccggttccgg atcctgcatc caaaacgcgg aggttttttt
     2881 tctgattaaa aaaatccgac ttttggattt ctgcaagcgg gatgtgcatc ttcgggaaat
     2941 taatgctgcc gtaataaagc agatatgcgt cacgaaaaac tttttgatca agatagatcc
     3001 ggcgaataaa ttcggaacgc ttgttttttt tcttaagtcc ctctgagatt tccgtgatcg
     3061 cttcagcaaa aggcttgagt gtccgtggaa tcggctcgtc aaggtgaagt tgcaaaaaat
     3121 cacccaggcg ctgaatataa tcgtcaggca aataaagcat taaccaattt cccgttcgca
     3181 aagaaatccg aaataatata gaacagtaaa gttcggcgat cccgttgcga tcattacgtc
     3241 gtagcgctga tgatatccat gcccatatat ttgcgcaaaa cctgcggcac catgatcgtg
     3301 ccttcatccg tttgataatt ttccataatg gcggcgacga tacgcggcaa ggccaagccc
     3361 gatccgttga gcgtatgcac aaactccggt tttcccgttt ggttgtcttt atatcgtata
     3421 ttcatgcgcc gtgcctgaaa cgattcaaaa ttggaacagg aggaaacttc cagccaacgt
     3481 ttttgtcctg cggcccaaac ttctaaatca tattttttag tttgcgtaaa acccatatca
     3541 cctcggcaca tcagcagtgt acggtatggt aactgtaatg cttgcaataa acgttcagcg
     3601 tccattcgca gcgattcgag ttcgtcgtac gaggtatcag ggtgaacaaa ttttacgagt
     3661 tctactttgt caaactgatg taagcggttc agtccgcgca catctttgcc gtacgaaccg
     3721 gcttcacgcc ggaaacaagg ggtataggcg gcatatttga ttggcaattg ttctgccttg
     3781 agcgtcatct cgcgatggat attggtaacc ggcacttccg cggtagggat cagaaaaaac
     3841 tcatcgcgtg tcacgacata catttgatct tctttatcgg gtaattgacc ggtaccggtt
     3901 gccgaatcgg tattgacgac gatcggcggc tgcacttcgg tataaccgtt atcggccgcg
     3961 gagtccagaa aaaaattaat caatgcccgt tgaagcttag cgccggcgcc gcggtaaacc
     4021 gggaaacctg caccggctat cttgacgccc gttgcaaaat caatcaaatt gtgtttttcg
     4081 gccaattccc aatgcggttt gagttcgaaa tccgccgacg gcatcgtacc ccatgtaaag
     4141 atcgtttgat tgtcttccgg tgaagtgcct tgcggcgcgg aagggtgagg tacattagga
     4201 atggtcagta atacatcgcg cagatttttt tcgatcactt tgagctgttc gtccaaagct
     4261 ttgatttctt cgccgacaga tttggtttcg gcgatgacgg catcggcatt ttggccggct
     4321 tttttgagtt tggccacttc ttcagaagct ttattgcgcc gggctttcag atcttcgact
     4381 ttacggataa caactttgcg ttcgtcatcc caacgaatca cggcatccac atcggcttca
     4441 aacttttttt ctttggccgc ggttttgaca cgttccgtat ggtcacggat aaatttcaaa
     4501 tcaagcatat ttcctcaaaa cattttgcaa gtgtgaacgt actgtatatt gactatacgc
     4561 caacatatca aaacttgccg tgtaattcaa atcgaaattg gatcccgaag tatgtagagc
     4621 ggttcaggtt ctgcagtttg ctcttgcaca aagcaacttt gacgaatatc ttttttctat
     4681 aaccgtcgaa aaaatcaaaa gaggttcgca tgtataaaac gatgtccgtg ttgatcgtta
     4741 tgggtttgtt gtgggcggcc tgccgccctt cggctttacc cccgttgccc aaagtatatg
     4801 atccgcattc caatgcccgc gccgaacagg cgtacgtaac acacatggcg cttgacgtac
     4861 gtgtggattt tgataataag cagatttcag gcagagcaac ctttgatgtg gtagtgtccg
     4921 at
//
LOCUS       DAOQNQ010000239         4901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_7261_length_4901_cov_5.397854, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000239 DAOQNQ010000000
VERSION     DAOQNQ010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7261_length_4901_cov_5.397854"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..2619)
                     /locus_tag="PK754_12460"
     CDS             complement(163..2619)
                     /locus_tag="PK754_12460"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNB10363.1"
                     /translation="MDLSKDELKEMMSIFKVESEEHLKNLNKGLLKLEETPNSRELID
                     ELFRTAHSIKGSARMMGFQKIEGVSHKIEDIFGLVRRAEIKVSPENFDVIYEGVDIIS
                     QIIELISNEGSDDSIDISVIVKKLEAIYNPQEAPASVAESKEESKPDVEPVLDVVSKA
                     EPVPVTEAKTPEPEETEFKPTTDLSKSEVSVDKTDAAKGNVSEDAFIRVPTRRLDDLM
                     NQVSELVTTRIKSQQRLADIRKMLDFTEEWAQQTERIRLLLDRTLSRIVNEDVERPSD
                     VKSELITSYDLRTVVTSYLSSIEKIGSIAESISILYDKQSEENLRMSVITGDIQDNLR
                     IIRLLPISTIFDLYPRMIRDIAKLQSKKVKFEVFGGDTRVDKKVLEELKDPLIHLLRN
                     SIDHGIEAPADREATGKAQEGTIQVRAGYVGNMVQIEVQDDGRGIDTDRILQIAIKKG
                     YIKEHQASEVSKNDLVGLIFHSGFSTAKIITDLSGRGVGLDIVKSNIEKIKGTIETVS
                     EKGKGTRFTIKIPLTLATTHVLIVDVGGDHYSLPIDFIDRTVRLTEEELWTGGQNPFV
                     LVDNTPVPLYKMEEVLGNAKQRLKNKGLLKSSAAPKIGQHLTKIETNKFPAVIFVSGG
                     RRVAFLVDKLLDEQEVVVKSLGHQLKRVKNVAGSTVLGDGRISIILDPNDLIKSVQGS
                     GTKFAFRDRRQRDLIKKRVLVVDDSITTRTLEKNILESAGYHVTIATNGLEGYQKLHE
                     IGNFDIVVSDVEMPFMTGFEFAHKVRTESKFPDIPFVLCTSLESDKDKRRGIEVGANA
                     YIVKGGFDQTNLLETIEKLL"
     gene            complement(2659..3885)
                     /locus_tag="PK754_12465"
     CDS             complement(2659..3885)
                     /locus_tag="PK754_12465"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB10364.1"
                     /translation="MIFLFFLLMAIPAAAIGTFVVYMVYGRKKFILSVLIRTVPWLVL
                     IVCAAFFIGIYSMHWWGWVIGLGAAVIFGVIYYLVLFRSTFTVTMDIVDVVTRIADKD
                     YSKRLTFKADGELNTMRESLNSVIDNLENTEKTLGDAVSRLSSVVSELNSTMDQQVVG
                     MNEQSTSLNQTTTTTQELAATSHQTTEKAQFVVESTERSMDITSKGKDAVDNTVDEMN
                     EIKKRVERIAEQILDLSEKTQQIGVITTTVNDIAEQTNMLALNAAIEASKAGEFGKGF
                     GVVAFEVRKLAEKSKEASLRIRDLITQIQNATNSTVMATEEGSKRVDIGVEKIREAGK
                     YMDESIQSLEESVGYAQQILVGSKQQTIGIEQITLAMANINEVVKQVAKGTAQTQGAV
                     DSVLGLTKEMDSLVKK"
     gene            complement(3904..4506)
                     /locus_tag="PK754_12470"
     CDS             complement(3904..4506)
                     /locus_tag="PK754_12470"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNB10365.1"
                     /translation="MAEPMKQQDVFDEVHKLMAELLSSRLTPEAEAKILAGRAEKPKR
                     AHTNEETGEQISVLIFQLSSEFFALETQYVSEVRVLSEVTPVPCTPDFVLGITNIRGA
                     VFSVIDIRGSFNLEDRPVTDRSMFLMIQWKGIELCLLADAVIEKINLPRSEVKTMTGG
                     NREHMSSFVSGFFIRNDNKITLISWDNYVSHSNIIVNEEV"
     gene            complement(4517..>4901)
                     /locus_tag="PK754_12475"
     CDS             complement(4517..>4901)
                     /locus_tag="PK754_12475"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB10366.1"
                     /translation="RALYMIAHINANLDNVSKAKDFCHMAIAEDSLMLEAYYLLGLIY
                     KEENLFDESVRMLKKTIYIDSDFAIGYYELAVNYFKLGDGVQARKYLKQTERILKNKS
                     DDERVGILDDLKVKELRMMVNMWDN"
BASE COUNT         1274 a         1215 c          969 g         1443 t
ORIGIN      
        1 cgtgactatg tccggccgga gaatctgaac tttttctacc gcctccctgc cgtttttagc
       61 cacgccgatc actcggcaat catcatccgt cgcaaaaatt ttctgaagga tcatcgtgac
      121 ggtcggtgaa tcttcagcga tgagtatctt gggcattgat tcctataaca acttttcaat
      181 ggtttcaagc agattagttt gatcaaatcc acctttgacg atataggcat tagcgccgac
      241 ctcgatcccg cggcgtttat ccttatccga ctccagtgac gtacacaata caaacggaat
      301 atccggaaat ttggactcgg ttcgcacttt gtgtgcaaat tcaaatcccg tcataaacgg
      361 catttccaca tcgctcacga caatatcaaa attaccgatt tcatgcagtt tctgataacc
      421 ttccaaaccg ttagtcgcga tagtcacgtg ataaccggcg gactcgagaa tatttttttc
      481 taatgtacgt gtcgtgatcg aatcgtccac taccaatacg cgctttttga tcaaatcgcg
      541 ctgacggcga tcccggaagg cgaatttcgt accggaacct tgaacggatt tgatcaagtc
      601 attgggatct aaaataatcg aaatccgtcc gtcaccaagc acggttgaac ccgcaacatt
      661 ttttacgcgt ttgagttgat gaccgaggct tttaacaacg acttcctgtt catctaaaag
      721 tttatccact aaaaacgcta cccggcggcc tccggaaaca aaaataaccg caggaaattt
      781 attcgtctct atttttgtaa gatgctgccc gattttgggc gcagcggacg atttgagcaa
      841 gcctttgttt ttgaggcgtt gtttggcatt gcccaacacc tcttccattt tatacaatgg
      901 caccggcgta ttatccacca atacaaaagg attttgcccg cccgtccaca attcttcttc
      961 cgttaatcgc accgtacgat ctataaaatc aatcggaagc gaataatgat cgccgccgac
     1021 atccacgata agcacgtgcg tagtcgcgag cgtgagcgga attttgatcg taaagcgtgt
     1081 accttttcct ttttcagaaa ccgtttcgat cgtgcctttg attttttcga tattggattt
     1141 gacgatgtca agccccacac cgcgcccgga aagatcggtg ataatttttg ccgtagaaaa
     1201 tccggaatgg aaaataagcc cgaccaaatc atttttactg acttcggagg cttgatgttc
     1261 cttgatataa ccttttttga tcgcgatctg caaaatcctg tcggtatcaa ttccacggcc
     1321 atcatcctgg acttcgatct gcaccatatt gccgacgtat ccggcacgaa cttgtatcgt
     1381 gccttcttgt gctttcccag tggcttcgcg atcggccggc gcttctatac catgatcaat
     1441 agaattacgc aataagtgaa tcagaggatc tttcaactcc tctaaaactt ttttatcaac
     1501 gcgcgtatcc ccaccgaaga cttcaaattt cacttttttg ctttgtaatt tggcaatatc
     1561 gcgaatcatg cgcggataca aatcgaaaat agtcgaaatc ggcaaaaggc gaataatgcg
     1621 caaattatct tgaatatccc ctgtgatgac ggacatgcgc agattttctt cactctgctt
     1681 gtcatacaaa atgctaatgg attcggcaat cgaacctatt ttctcaatgg atgagagata
     1741 cgaagtaact accgttcgca aatcgtagga tgtgatcaat tcgcttttta cgtcacttgg
     1801 tctctcgacg tcttcgttga cgatacgtga taaagtacga tctaacaata atcgaatgcg
     1861 ttccgtttgt tgagcccact cctcggtaaa atccagcatt ttgcgaatgt ccgccaatcg
     1921 ttgctgggat ttgatgcgcg tagtgacaag ttcactcacc tgattcatca gatcgtccaa
     1981 acgccgcgtc ggcacacgaa taaacgcatc ttcggaaaca ttgcctttgg ccgcgtcggt
     2041 tttatcaaca ctcacctccg acttggatag atcggtcgtc ggtttaaatt cggtctcctc
     2101 cggctcagga gttttcgctt ccgtcacggg taccggttcg gctttagaca caacgtccaa
     2161 caccggttcc acatcgggtt tagattcttc cttcgactcg gctacggaag ccggtgcttc
     2221 ttgcgggtta taaatagctt ctaatttttt tacgataact gaaatatcga ttgaatcgtc
     2281 cgatccttcg ttggatatca attcgataat ttgagaaata atatcaaccc cttcataaat
     2341 gacatcaaaa ttttcgggtg atactttgat ttccgcgcgg cgtactaaac cgaaaatatc
     2401 ttcgattttg tgggataccc cttcgatctt ctgaaaaccc atcatacgcg ccgatccctt
     2461 gatgctgtgc gccgtacgaa aaagctcatc aatcagttct cgggagttgg gcgtttcttc
     2521 aagtttgagc aaacctttat taaggttttt cagatgctct tcgctctcca ccttgaagat
     2581 ggacatcatt tctttgagtt cgtctttaga caaatccata ggcaaatccg taaaatatgt
     2641 ggcccggatg atgaacgatt attttttgac taaactatcc atttctttgg tcaatcctaa
     2701 cacggaatcc acggcacctt gtgtttgcgc ggttcctttg gcgacttgct tcacgacttc
     2761 gttgatatta gccatcgcta gcgtgatctg ttcgataccg atcgtctgtt gtttgctgcc
     2821 aaccaaaatc tgttgggcat aacctacgct ttcttccaaa ctttggatgg attcatccat
     2881 atattttccg gcttctcgta ttttttcaac accgatatca acgcgctttg agccttcttc
     2941 cgtagccata actgtcgaat tcgttgcatt ttgtatctgg gtgatcagat cacgtatgcg
     3001 cagagaagcc tctttgcttt tttcagctag cttccggact tcaaaagcca ccacaccgaa
     3061 tcctttgccg aattcgccgg ccttactcgc ttcgatagcc gcattcagcg caagcatatt
     3121 ggtttgttcg gcaatatcat tgacggtggt ggtgataacg ccgatctgtt gcgttttttc
     3181 gctgagatca agtatttgct ccgcgatacg ttcaacacgc tttttgattt cattcatttc
     3241 atccacggta ttgtctaccg catctttgcc tttggacgta atatccatac tgcgctcggt
     3301 actctctacg acaaactgag ctttttccgt tgtctgatgc gaagtggcgg ccaattcttg
     3361 tgtcgtggtt gtcgtctgat tgagcgatgt agattgctca ttcatcccca caacctgttg
     3421 atccatcgtc gagttaagct cactgacaac cgatgacaaa cgcgatacgg catcgcccaa
     3481 agttttttcc gtattctcca aattatcgat gaccgagttg agactttcac gcatggtatt
     3541 caattcccca tcggccttga atgtcaaacg tttggaataa tctttatccg cgatacgcgt
     3601 tacgacatcc acgatatcca ttgtaactgt aaaagtactg cggaatagca ccagataata
     3661 aataacgcca aaaattacag ccgcgcccag gccaataacc cagccccacc agtgcatcga
     3721 atatataccg atgaaaaagg ccgcacatac gatgagtacc aaccagggta cggtgcgtat
     3781 caaaacgcta aggataaatt tttttcttcc gtaaaccata taaacaacga aagtgcctat
     3841 ggctgcagca ggtatcgcca tgagtaaaaa aaacaaaaaa atcatatatg gattctccgg
     3901 agtttagact tcttcgttga caattatatt cgaatgtgaa acatagttat cccaactgat
     3961 caacgtaatt ttattatcat tgcgaataaa aaaaccgctc acaaatgacg acatgtgttc
     4021 tctattgccg ccggtcatcg tcttgacttc cgaacggggt aaattgattt tttcaatcac
     4081 ggcgtcggct aataggcaca gctcaattcc tttccactga atcatcaaaa acatagatcg
     4141 atccgtgaca ggccggtctt ccaaattgaa tgaaccgcga atatcaatca ccgaaaatac
     4201 ggcaccgcga atattagtta ttccgagcac aaaatccggc gtacagggaa ccggcgtcac
     4261 ttcgctgagt acccttactt cagacacata ctgcgtttct aaagcaaaaa attcagacga
     4321 taactggaaa ataagcacac taatttgttc gcccgtttct tcgttggtat gcgcacgttt
     4381 gggtttttcg gcacggccgg ccaaaatttt agcttcagcc tccggcgtca agcgtgacga
     4441 caaaagttct gccatcagct tgtggacttc gtcaaataca tcttgttgct tcattggttc
     4501 cgccatacgt ttatcactaa ttatcccaca tattaaccat catgcgcagt tctttgactt
     4561 tcaagtcatc caaaatccct acgcgctcgt cgtccgattt attttttaaa atacgctcgg
     4621 tttgttttaa atattttcgc gcttgcacgc cgtcaccgag tttaaaataa ttgaccgcca
     4681 actcgtaata cccgatcgca aaatcagaat caatatagat cgtcttctta agcatgcgca
     4741 ccgattcgtc aaaaagattt tcttctttgt aaatcaatcc caacaggtaa tacgcttcca
     4801 gcatcaggga atcttcggcg atcgccatgt ggcaaaaatc tttggcttta ctgacattat
     4861 cgagattggc attgatgtgc gcgatcatat acagcgctct a
//
LOCUS       DAOQNQ010000240         4886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_7298_length_4886_cov_5.856344, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000240 DAOQNQ010000000
VERSION     DAOQNQ010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7298_length_4886_cov_5.856344"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1816)
                     /gene="rpsA"
                     /locus_tag="PK754_12480"
     CDS             complement(101..1816)
                     /gene="rpsA"
                     /locus_tag="PK754_12480"
                     /inference="COORDINATES: protein motif:HMM:NF004953.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNB10367.1"
                     /translation="MSQTTNQARDKFHGKAPLPFVKVNEAIGDYSESEFLDLKTMYDR
                     TLSEISQSTIVKGKIRAINEKEVKVDIGFKSEGIIATTEFGSNLDKYKVGDEIEVYLD
                     NMEDKEGNVILSHKKADFMRSWEKINRLHDSGETVSGTIAKRIKGGMVVSVMGIDCFL
                     PGSQIDVKPIRDFDAYVGREMNFKVVKINNLRKNVVVSSRVLIEESTKGQRERILTDL
                     QKGQILEGTVKNITDFGAFIDLGGVDGLLHITDMSWGRVKHPSEIVKLDEKIKVMVID
                     FNEHKDRISLGLKQMQPEPWLEAAKKYSIGSMVKGKIVSITDYGAFIEIEKGIEGLIH
                     ISEMSWNQTIKHPSQMFNLGDEVEAEVVNIQADQRKISLSIKKLQNDPWEHVDDRYPL
                     GSKHAGTVRNLTNFGVFVELEPGIDGLVHISDLSWTRKIRNPNEMVKKGDHLDVVIMG
                     IDKDNRRISMSHKHVTENPWDGFIQAYAASTETTGKIMRHIEKGVIVELPLGVEGFVP
                     TSHLPKSDKKDAYGEGVELNLVVIEFDKDNKKIVLSATELEKQKEAKMVEEYNASQKG
                     GEAQA"
     gene            complement(2051..3253)
                     /locus_tag="PK754_12485"
     CDS             complement(2051..3253)
                     /locus_tag="PK754_12485"
                     /inference="COORDINATES: protein motif:HMM:NF022694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HNB10368.1"
                     /translation="MSNIRRVLFILLCFQAPLFAQAQVSTDALKSQLTSLRELLRDTA
                     RFRSVHAPVLMDQLMDWNEITRRKFEANWVNLTKSWQLRFERAIRQRLSEVFTQYLIH
                     NAATLRGAESRWFDEEIGITRAKASLEIVQNQEIVRIALRLSFSENRWRIYDIRTPEF
                     RLLRDLLPDCDEKISDGYSNEYVEALITNAEHFVIDDFNAVESGVFPRNWGWRKRDDD
                     LMRSSQRVYSVVKDSQDSYLSAIARGTSVALVRPYSYDLREYPVLTWKWRVNTMPSAS
                     SQNDAIENAASVTVIFYQNWLGVPITITYAWAYEGGTCTTVKTSGWLYDNFSIVLRNA
                     QSPHGQWIEETINLREDYKRIFGEYPPDQIAGLYIITDSPNFQFPTTADYDHIRAQKN
                     TTAYSCRN"
     gene            complement(3250..3987)
                     /locus_tag="PK754_12490"
     CDS             complement(3250..3987)
                     /locus_tag="PK754_12490"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNB10369.1"
                     /translation="MIQKPSFFISDIHFMGGRSAEELHKYRMVKSFFEHVAEYGQELF
                     ILGDYFDFGIDYRHSLPSQNVRGLALLVLLADAGVQIHYLVGNHDFWLGDFLQKELDI
                     KLYYEPFSISRNGKNIYLIHGDGLSDLDKSYRAMRRILRARFNQFLFRWLHPDLGIAL
                     ANRMSHASKNQDVLYTKYNQDESYFKPLSNIFDQGYDMILMGHHHQPMMRVFNNKIYY
                     NLGDWIQHGSYLESDAQGFSQKTWKLT"
     gene            complement(3990..>4886)
                     /gene="lnt"
                     /locus_tag="PK754_12495"
     CDS             complement(3990..>4886)
                     /gene="lnt"
                     /locus_tag="PK754_12495"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNB10370.1"
                     /translation="RVAIIQPNIEPNRKWLEHDFAYEELLRLTQSIPEGKTDLIIWPE
                     TAIPVRLRQDRTKLAAIREILKEKKASLLTGIPDRKMTESEDGRRSAEYYNAALVIRP
                     DNTQLGNYEKVHLVPFGEFVPSFLKFLDAIAMEVGASNYTPGDSVHAFTIPLYSDSIP
                     TDSACITAVICLESIFPYLVREGLERDAQALVIVTNDSWYDGTMAPEQHARIAVLRAI
                     EFRTAVARCANSGVSNAIDPYGRITAKLDNGLQGVLYATIPLQTSTSIYMRWGDWFAH
                     SLWMIITLSLCFLFFYKPSKRD"
BASE COUNT         1289 a         1094 c         1127 g         1376 t
ORIGIN      
        1 attttaatag cggatgactg ctcatagttc aaacggttaa aagaaaaggg ttgaacggct
       61 tacgctattc aaccctttac acattcatta cggctgaaga ttaagcttgc gcttcgccgc
      121 ctttttggct ggcgttgtat tcttcgacca ttttagcttc tttttgtttt tcaagctctg
      181 tcgcggacag gacgattttt ttgttgtcct tgtcaaattc gatcacgact aagttgagtt
      241 ctacaccttc gccataagcg tcttttttat cggatttcgg caagtgcgac gtcggtacaa
      301 aaccttcgac gccgagcggc aattcgacga tcacaccctt ttcgatatga cgcatgattt
      361 tgccggttgt ttccgtactg gcggcataag cctggataaa gccatcccac ggattttccg
      421 tgacatgctt gtggctcatg gagatacggc gattatcttt atcaataccc atgatgacga
      481 catccaaatg atcccctttt ttgaccattt cattgggatt gcggatctta cgagtccatg
      541 ataaatcgga aatgtgaact aaaccgtcaa taccgggttc gagttccaca aaaacaccga
      601 agttggtaag attacgtact gtaccggcgt gtttgctgcc gaggggataa cggtcatcca
      661 cgtgttccca aggatcattt tgcagttttt tgatcgagag ggatattttg cgctgatcgg
      721 cctggatatt gacgacttcg gcttctactt catcgccgag gttgaacatc tgcgaaggat
      781 gtttgatcgt ttgattccat gacatttcag aaatgtgaat gaggccttcg ataccttttt
      841 cgatttcgat gaaggcgccg tagtcggtga tcgaaacgat tttacctttg accatcgaac
      901 cgatggaata ttttttagcg gcttcgagcc acggttccgg ttgcatttgt ttgagaccga
      961 gggaaatgcg gtctttgtgc tcgttgaaat caatgaccat gactttgatt ttttcatcaa
     1021 gcttgacgat ttccgaagga tgtttgacgc gcccccagga catatcggtg atgtgcaata
     1081 atccgtccac accgccgaga tcaataaacg caccgaaatc ggtaatgttt ttgaccgttc
     1141 cttcaagaat ctgacctttt tgtaaatccg taaggatacg ttcacgctgg cctttggtgg
     1201 attcttcgat gagtacgcgg ctgctgacaa ccacattttt gcgcaggttg ttgatcttaa
     1261 cgactttgaa attcatttcg cggcctacat acgcatcaaa atcgcggatc ggcttaacat
     1321 cgatttgcga gccggggagg aagcagtcaa tacccatcac gctgacaacc ataccgccct
     1381 tgatacgttt ggcgatggta ccgctgacag tttcaccgct atcatgtaag cggttgattt
     1441 tttcccagga acgcataaag tcggcttttt tgtgtgaaag aatcacattg ccttctttat
     1501 cttccatatt gtcaagataa acttcgattt catcgccgac tttgtatttg tcgaggttgg
     1561 aaccaaattc ggttgttgca ataatgcctt cgcttttgaa tccgatgtcc actttgactt
     1621 ctttttcatt gatggcacgg attttacctt tgacgatcgt gctttgtgaa atttcactca
     1681 gggtgcgatc atacatggtc ttgaggtcaa gaaattccga ttcggaataa tcaccgatgg
     1741 cttcattgac tttgacgaaa gggagcgggg ctttgccgtg aaatttgtca cgggcttggt
     1801 ttgtggtttg tgacatgata acttttattt gctgatgttt atccgccgga tgagcagacc
     1861 acagggtgca tccgcaaaat ttccttttac tggctccgcg taaacggagg tttggttagg
     1921 cgtccttgtg tacgctgttg ttattaaatc ccattttagg ggcaggaaga tagaaaatca
     1981 gagttatgca agcaaggaaa atttgtatct aaaatagaag gcttggaagg cgggttacgg
     2041 aggtaaattt tcaatttcgg cacgaatatg ccgtggtgtt tttttgagcc cggatatgat
     2101 cgtaatcggc ggttgtcgga aactgaaaat tggggctgtc cgtgataatg tacaacccgg
     2161 cgatttgatc cggcgggtat tcaccgaaaa tacgtttata atcttcccta agatttatgg
     2221 tctcttcgat ccattgaccg tgcggcgatt gggcattgcg taaaacgatc gaaaagttat
     2281 catataacca acccgaagtt ttgaccgtcg tacaagtgcc gccttcatag gcccaggcat
     2341 aggttatggt gatcggtacc ccgagccaat tttgataaaa aatgacggtt accgaggcgg
     2401 cgttttcgat cgcatcgttt tgagatgaag ccgatggcat ggtattgacg cgccatttcc
     2461 atgtgagtac aggatattca cggagatcat aactgtaagg ccgaactagc gcaacggatg
     2521 tgccgcgggc tatggcggat aaatagctgt cctgactgtc tttgacgaca ctatagacgc
     2581 gctgtgaaga gcgcatcaga tcatcgtccc gtttgcgcca accccaattg cgcggaaaaa
     2641 cgccagactc aacggcgtta aaatcatcaa tgacaaaatg ttcggcgttg gtgatcagcg
     2701 cttctacgta ttcattgcta tatccgtcac tgatcttttc atcgcaatcc ggtaaaaggt
     2761 cacgcagcaa acgaaattcg ggcgtgcgta tatcgtatat tcgccatcgg ttctcggaaa
     2821 aagaaagccg caatgcaatg cgtacgatct cttgattttg tacgatttcc agagacgctt
     2881 tggcacgcgt tataccgatc tcttcgtcaa accaacggga ctcggcaccg cgcagcgttg
     2941 ctgcgttgtg tatcaggtac tgcgtaaaaa cctcgctaag acgctgtcgt atcgctcttt
     3001 cgaaacgtaa ctgccagctt ttggtaaggt tcacccaatt ggcttcaaat tttcgacgtg
     3061 taatttcatt ccaatccatc aactggtcca tcaaaaccgg agcgtgcacc gaacggaagc
     3121 gcgccgtatc tcttaatagc tcgcgtaggg aagttaactg cgacttcaat gcgtccgttg
     3181 aaacctgtgc ttgtgcaaag agcggcgctt gaaagcataa caggataaac aatactcgtc
     3241 gaatattact catgtaagtt tccaagtttt ttgcgaaaac ccctgcgcgt cggattcaag
     3301 gtaactgccg tgctgaatcc agtctcccaa attataatat attttattgt tgaaaacacg
     3361 catcataggc tgatgatgat gtcccatcaa tatcatatca tagccctgat caaaaatgtt
     3421 actcaacggc ttaaaatagc tttcatcctg attgtatttg gtatataaaa catcctggtt
     3481 tttgcttgcg tgcgacatac gattggctaa ggcgataccg agatcgggat gtaaccatcg
     3541 aaataaaaac tgattgaaac gagcgcgcaa aatgcggcgc atcgcacgat aggatttatc
     3601 aagatcggat aatccgtcac cgtgtataag ataaatattt ttcccgttac gtgaaattga
     3661 aaatggctca taataaagtt taatatccaa ttccttttgc aaaaaatcac cgagccaaaa
     3721 atcatgattg ccgacaagat aatgaatttg tactcctgcg tctgccaata ataccagcaa
     3781 cgcaaggccg cgaacgtttt gcgaaggaag gctgtggcgg taatcaatcc caaaatcaaa
     3841 ataatccccg agtataaaaa gctcttgtcc atattcagcg acgtgctcaa aaaaagattt
     3901 gaccattctg tatttatgaa gttcctctgc tgagcgcccg cccataaaat gaatatcgga
     3961 aatgaaaaat gaaggctttt gaatcatggt caatcgcgct tcgacggttt ataaaaaaac
     4021 aaaaagcaca gcgataaagt gatgatcatc caaaggctgt gagcgaacca atcgccccat
     4081 cgcatataaa tgctggtact ggtttgcaac ggtatcgtcg catacagcac tccctgcaag
     4141 ccattatcca acttagccgt aatgcgtccg tagggatcaa tggcattgga gacgccggag
     4201 ttggcgcacc gggctaccgc agtgcgaaac tcaatcgccc tcagaacggc gatgcgtgcg
     4261 tgttgttcag gcgccatcgt accatcatac catgaatcat tggttacaat aaccaaagct
     4321 tgcgcatccc gctccaaacc ttcccgaact agataaggga atatggactc aaggcaaatg
     4381 acagcggtaa tacacgcgga gtcggtcggg atgctatcgg aatagagagg aatggtaaaa
     4441 gcatgaactg aatcgccggg cgtatagtta gaggcaccga cttccatggc gatggcgtcc
     4501 aaaaacttca aaaaactcgg aacgaattcg ccgaaaggaa caagatgaac tttttcataa
     4561 tttccaagct gcgtattatc cgggcgaata acaagtgcgg cgttataata ttcagccgac
     4621 cgtcgtccgt cttccgattc tgtcattttc cgatcgggta ttccggtgag cagagaggct
     4681 tttttttctt ttaagatttc acgtatcgct gcgagtttag ttcgatcttg tctcaatcga
     4741 acaggaatgg ccgtttccgg ccaaatgatc agatcggttt tgccttcagg aatagattgc
     4801 gttaaacgca acaattcttc gtaagcaaaa tcatgctcaa gccatttgcg attcggttca
     4861 atattgggtt gtataatcgc tacacg
//
LOCUS       DAOQNQ010000241         4880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_7315_length_4880_cov_7.304041, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000241 DAOQNQ010000000
VERSION     DAOQNQ010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7315_length_4880_cov_7.304041"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PK754_12500"
     CDS             complement(<1..645)
                     /locus_tag="PK754_12500"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNB10371.1"
                     /translation="MNKFEYLQSASLDKLLPVLQEPDTAIKAGGVDIIDLMKEGLTAP
                     SRLVSLRPITALQKISLDDDLSIGAGATIADISNVNNIYAGPFRVLAQACASLATPQI
                     RHAATIGGNLCQRPRCWYFRSHEFDCTRKGGKICYAMNGENAYHAIFGNTDGCVIVHP
                     SSPAVALTALDARLKIKSSKGERELAMDQFFITPANDITKENALQPGEVITEIYI"
     gene            complement(688..2973)
                     /locus_tag="PK754_12505"
     CDS             complement(688..2973)
                     /locus_tag="PK754_12505"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNB10372.1"
                     /translation="MPKNIKLKTGHSGVWTEKNVSLPDNVPKPWDADDRLQLVSKNQT
                     RLDGQAKVTGSARYTFDIQLPDMLHARILRSPHASAVIRKIDTSRTEKYPGVRAVIRI
                     QDTIPQFVRFAGQEICAVAATSTHAANEALKLIEIQYEKRGVITTIQDAMKPGASHIF
                     DTAVEEQKSEGDEPTSIGSVTQKGNLRGPAVKGNAASVDAALSSSAVIVDAVYETQVQ
                     THSAMETHGVVAFWENSDKLTVWASTQGTFSVRDELATVFQLPKTHVRVITEYMGGGF
                     GAKFGAGVYGIAAAKLAKIAGAPVRLMLDRKEEHIAAGNRPSAVQKLRVGANARGEIQ
                     AIKLIAHGTAGVGTGAGCDGPAMNLYFAVPHKYSEQYDVFTHAGAGAAFRAPGHPQGT
                     FALEQSVDELAYKLNMDPLALRRINSKTDSVRLAEYDIGAKRFGWDRRAAQPAGDKGP
                     IKRGFGMANAVWYYIYGTGFQATIEVHSDGSVDLTNGVQDIGGGIRTALAMIVAEELG
                     LQTKDIVIKIGDTQYGLGPASGGSQTTAGITPAARHAAYLAKLKMFEIAAPMLGVPSD
                     DLSVADGRIFSRRDTSKSLAWKQVASKISGDKFSVIGERARDYLTLSPPYIAGVQFAD
                     IEVDTETGIIKVNRIVAVHDCGRVINRLTLESQIRGGVIQGLSYALFENRILDAYTGR
                     MVNANFDQYKIAGAKDIPVIETEILDVYTGASATGAMGIGEPATIPTAAAIANAFFHA
                     TGKRIRKLPMHAAHVLEALQS"
     gene            complement(2992..3630)
                     /locus_tag="PK754_12510"
     CDS             complement(2992..3630)
                     /locus_tag="PK754_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNB10373.1"
                     /translation="MKEPQDDAGELPDPSRRAFLKDFGLSSLGAVAANGGIIGALQAS
                     CANPTQDIQTLGPGATDITLSVNGKKQSLRVEPRWTLAEVLRDHLHLTGTKTGCERGA
                     CGACTVWMNDKTINACLTLAVDAIGSEITTIEGLSDGMSLHPLQQAFIEHDALQCGFC
                     TPGMVMSCAALLRDNKNPSIEDVKDAVSGNLCRCGTYNHVFEAVQSAAKNMK"
     gene            complement(3730..4194)
                     /locus_tag="PK754_12515"
     CDS             complement(3730..4194)
                     /locus_tag="PK754_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008275003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB10374.1"
                     /translation="MAIHIRLATVSDTDILVHNNAAMSKETENKELPRHILRDGVTNL
                     FRQPQFGFYLLAERPDGTVVGQLMITYEWSDWRNGVFWWIQSVYVDPEFRGMGIYRKL
                     YQYVLEQARTKKDVCGIRLYVDKSNRKAMNVYHRCGMNEAHYAMYEVDFVLG"
     gene            4275..>4880
                     /locus_tag="PK754_12520"
     CDS             4275..>4880
                     /locus_tag="PK754_12520"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB10375.1"
                     /translation="MHFADAYCLSRYRHLYLQMTMKISVIIPAYNEASIIGETLSALM
                     KADRPSNPIEFIVVNNASTDRTAEIASSFPDIRVVTESEKGLTKARQCGNAHAQGDIL
                     IYIDADTRIPKHLLRTIEEKFIADPKLVAMSGPYKYHDWTWYGRLILWLYHWTLVPMN
                     QLVINRILNKGSVFYGGNFAVRKKALDKIGGFDTGLEFWSED"
BASE COUNT         1161 a         1198 c         1209 g         1312 t
ORIGIN      
        1 tatgtatatt tccgtgatga cttcaccggg ttggagtgcg ttctcttttg taatatcgtt
       61 cgccggggta atgaaaaatt gatccatagc cagttcgcgt tcacctttgg acgattttat
      121 tttcaggcgc gcatctaacg ctgtcaaagc cacggccggc gacgaaggat gaacgataac
      181 acagccgtcg gtatttccaa aaatcgcatg atacgcgttt tcgccgttca tcgcgtaaca
      241 gatttttccg cctttgcgtg tacaatcaaa ttcgtgactt ctaaagtacc agcagcgtgg
      301 cctttggcag agattgcctc cgatcgtagc ggcatggcgg atttgcggtg tcgccaaact
      361 tgcacaagcc tgagccaaaa cgcgaaacgg gccggcgtaa atattattta cattggaaat
      421 atccgctatc gtagcgccgg ctccgatgga tagatcatca tccagagaaa ttttttgtaa
      481 cgctgtgatt ggacgcagac tgactaagcg cgaaggcgct gtcaatcctt ctttcatcag
      541 atcaatgatg tccacgccgc cggcttttat cgcggtgtcc ggttcttgaa gtacgggcaa
      601 aagtttgtct aaacttgcac tctgtaggta ttcaaattta ttcatatgta caaacctttt
      661 ggtcttgctg aagtgtcaga tgtttggtta cgactgaagc gcttccagta catgggcggc
      721 atgcataggg agtttgcgaa tgcgtttacc cgttgcgtga aaaaaggcat tggctatagc
      781 cgccgccgtc ggtatcgtag caggttcgcc gatccccata gcgcccgtag cggaagcgcc
      841 ggtgtagaca tccagtattt ccgtttcaat gaccggtata tctttggctc cggcaatttt
      901 gtattgatca aaattggcgt tgaccatacg accggtatac gcatctaaaa ttctgttttc
      961 aaataaagca taactcaatc cctgtatcac accgccgcga atctgactct ccagtgtcag
     1021 ccgattgatc acacgaccgc aatcgtgcac cgcgacgata cgattaacct tgataatgcc
     1081 ggtttcggta tccacttcga tatctgcaaa ttgtacaccg gctatgtacg gcgggctgag
     1141 tgtaaggtaa tcgcgcgcac gttctccgat aaccgaaaat ttatcaccgc tgattttaga
     1201 ggcaacttgt ttccatgcga gactttttga cgtatcgcgt cgggaaaaaa tgcggccgtc
     1261 ggcaacggac aaatcatccg acggtacacc cagcataggt gcggcgattt caaacatttt
     1321 gagtttagca agataagcgg catgacgtgc tgccggggta atacccgccg tagtctgact
     1381 accgccgctg gcggggccga ggccatactg cgtatcgccg attttgatga cgatgtcctt
     1441 cgtttgcaat cccagttctt ccgcgacgat catcgccaaa gcggtgcgga taccgccacc
     1501 gatatcttga acgccgttgg tcaaatcgac ggaaccgtcg ctatgcactt cgatggtagc
     1561 ttgaaatccg gtaccgtaaa tgtaatacca tacggcgttg gccattccga aaccgcgctt
     1621 gatcgggcct ttgtcgccgg cgggttgcgc ggcgcgccgg tcccaaccga aacgtttcgc
     1681 gccaatgtcg tattcagcca atcgaacact atcggtttta gaattgattc gtcgtagggc
     1741 gagcgggtcc atattgagtt tgtaggccaa ttcatctacg gattgctcca gtgcaaatgt
     1801 tccctgcgga tgacccggcg ctctaaatgc ggcgcctgca ccggcatggg taaagacatc
     1861 gtactgctcg gagtatttat gaggaacggc aaaatacaaa ttcatcgccg gaccgtcgca
     1921 tccggcgcct gtaccaacgc cggccgtccc gtgcgcaatc aatttgatgg cttggatttc
     1981 acctcgcgcg ttagcgccga cacgaagttt ttgcactgcg ctggggcgat tgcctgcagc
     2041 gatatgttct tcctttcggt ccagcataag gcgaacaggt gcaccggcta ttttggccaa
     2101 tttggccgca gcgatgccgt atactcctgc accgaacttt gcaccaaatc caccgcccat
     2161 gtattccgta atcacgcgta catgtgtttt gggtaactga aagacagtgg ctaattcatc
     2221 ccgaacactg aatgtgcctt gtgtcgaggc ccacaccgta agtttgtccg agttttccca
     2281 gaacgccacg acaccatgcg tttccattgc cgaatgggtt tgcacttgtg tttcataaac
     2341 agcatctacg attacggcgc tggatgataa cgcggcatcc acagaagcgg cattgccttt
     2401 gacggcgggg ccgcgcaagt tgcctttttg cgtaacggat ccgatggatg tcggttcatc
     2461 gccttccgat ttttgttctt caacagctgt gtcaaaaata tgggaagcgc ccggtttcat
     2521 cgcatcctga atcgtggtaa taaccccacg tttttcgtat tgaatttcga tcagttttag
     2581 cgcttcgttg gcggcgtgtg ttgacgtcgc ggctacggca caaatttcct gaccggcaaa
     2641 tcgtacgaat tgtggtatcg tgtcttgtat acgtatcacc gcgcgcacgc cgggatattt
     2701 ttcggtgcgg gaggtgtcta tcttgcgaat gacggctgaa gcgtgtggtg agcgcagaat
     2761 ccgagcatgt aacatgtccg gcaattgaat gtcgaaggta taccgtgcag aacctgttac
     2821 tttggcctga ccgtccagac gagtttggtt tttgctgacc aattgtaaac gatcatccgc
     2881 atcccatggc ttcgggacgt tatcgggaag ggaaacgttt ttttccgtcc atactccgga
     2941 atgaccggtt ttgagtttga tatttttagg cataaaaact actcaatttt gttatttcat
     3001 attttttgca gccgactgta cggcttcaaa aacgtgattg tatgtaccgc atcggcaaag
     3061 gtttccgctg accgcatctt ttacgtcctc gatggacggg tttttattat cgcgaagtaa
     3121 agctgcgcaa ctcatcacca tacccggtgt acaaaatccg cattgcaaag cgtcatgttc
     3181 gataaaagct tgctgtagcg ggtgcagcga cataccatcc gaaagccctt cgatcgtcgt
     3241 aatctcggac ccgatcgcat ccacagccag agtgaggcat gcgtttatgg ttttatcatt
     3301 catccatact gtacacgcac cgcaggctcc gcgttcacat cccgttttgg tgcctgtaag
     3361 atggagatga tcacgcagca cttcggccaa tgtccagcgc ggttcgacac gcaaggattg
     3421 ttttttgccg ttgacggata atgtgatgtc cgttgcaccc ggacccagtg tttggatatc
     3481 ctgcgtcgga ttggcacagg aagcttgcag tgcgccgatg ataccgccgt tggcggcgac
     3541 agcgcccaga gatgagaggc cgaaatcttt gaggaaagcg cggcgtgatg gatccggcaa
     3601 ttcaccggca tcgtcttgcg gttctttcat aagctgctct tcaaagttgg tatggtgttc
     3661 gatatgttaa gttttattaa cggaaaaatc agggttaatg tttcagaatc gcaaagttgt
     3721 tgtgatccat caacccaaaa caaaatccac ctcatacatt gcataatgcg cttcattcat
     3781 accgcaacga tgatatacat tcatggcttt gcgattactt ttgtccacat atagtcgtat
     3841 gccgcacacg tcttttttag tgcgcgcctg ctcaagtaca tactggtaca atttgcgata
     3901 tatacccatg ccgcgaaatt ccgggtcaac atacacgctt tgaatccacc agaaaacccc
     3961 gtttcgccaa tcgctccatt cgtacgtgat catgagttgt ccgacgaccg tgccatcggg
     4021 ccgttcggcg agtaaataaa aaccaaattg cggctgacga aacagattgg ttacgccatc
     4081 gcgtaaaata tggcgcggta attctttatt ttcagtttct ttggacatag cggcattatt
     4141 gtgaacaagg atatccgtat cggaaaccgt tgctaaacgt atatggatgg ccataaagtt
     4201 aatgcagtta taatgaaggg attatagcgc tataatacgc ttttcctaat ttggttacaa
     4261 atagagaata accggtgcac tttgcagacg cttattgcct tagtcggtat cggcacttat
     4321 atttgcagat gacgatgaaa atcagtgtga tcatacccgc ctacaacgaa gcctcgatta
     4381 tcggtgaaac gctttcggca ttgatgaaag ccgaccgtcc atcgaacccg atagagttta
     4441 tcgttgtcaa taacgccagc acggatcgta cggcggagat tgcctcttcg tttcccgaca
     4501 tccgtgtcgt tacggaatcg gaaaaaggtc ttaccaaagc gcgtcagtgc ggcaacgccc
     4561 atgcgcaagg cgatatcctg atctatattg atgccgatac gcgcattcct aagcaccttt
     4621 tacgaacgat cgaagaaaaa tttatagccg atcccaaact tgttgcgatg tcgggccctt
     4681 ataagtatca cgactggacg tggtacggac ggttgatctt gtggctttat cactggactt
     4741 tggttccgat gaaccaattg gtgatcaatc ggatactcaa taagggtagt gttttttacg
     4801 gcggtaattt tgccgtgaga aaaaaagcgc tggataaaat aggcggcttt gataccgggt
     4861 tggaattttg gagtgaagat
//
LOCUS       DAOQNQ010000242         4870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_7344_length_4870_cov_5.341225, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000242 DAOQNQ010000000
VERSION     DAOQNQ010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7344_length_4870_cov_5.341225"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1404
                     /locus_tag="PK754_12525"
     CDS             <1..>1404
                     /locus_tag="PK754_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNB10376.1"
                     /translation="EGMSFIPPYVFWLIVCLLVSGFLSSRIIRLSRNEKWRQFFIVEL
                     IITIVISVLLVYFYFYGGTGNVRLDRSLKNMIMISSLIPTGAMVYLLYKYPFYSTVAR
                     RRFLLITISGVFLVVFIMSSKALRKMAEENPELNVEFLEIVLVSMLFLVYEPTKYLVR
                     RIAGQFALHEKSYFQSLIRKITEHIVSAANLAELTKAVRTGIVDALHVEEVGLYTLNK
                     TVSGERTLYSIVHAYGDIKTFNLEKITRHIQKGNGLYDARKMNIFHLRSAEVPYQIYL
                     GIVLDNELVGILAIGKKKSNEDFEFEEKELLLTVASQVAISIENIQLLQRRIELESKM
                     FEADKLTSLGMLSTSIAHEVKNPLSSIKSIVQSMHDQKKKENTDTEAIQDLEIINEEI
                     DRLSSVVNQLLRFARSDSSGMDRIDIIKIIDAILTISRQETRVRNIRIFTRYDHAPLV
                     IRSRQGDLKEIIFNLII"
     assembly_gap    1405..1504
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1678..3126
                     /locus_tag="PK754_12530"
     CDS             1678..3126
                     /locus_tag="PK754_12530"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNB10377.1"
                     /translation="MSDIPVESSQEHTSHIRILVVDDEKAARYGMLKALKPEGYKLSE
                     ADDGLSALNLMANNPFDIVITDVSMAGMNGMDLLKKIKERTPHCFVIVITAHGSERLA
                     VEAIKSGAFNYLAKPYDIEELRALVSHAAQQIAVVHENERLRAELKEAKGFGQFVGSS
                     KPMRQVFDLLERVSRNNITVLIRGESGTGKELVARTIHEKSNRRSGPFISINCAALPK
                     ELIESELFGYEKGAFTGAISSKQGRFELADGGTLFLDEIGDMSLETQAKVLRVIQERK
                     FERVGGTESISVDVRILSATHRDLARFIRDGQFREDLYYRINVVEIELPPLRSRSEDI
                     HLLVNHYLVMFAQKHKAQVTRFSQDALKAMLEYDWPGNVRELINVVERCVVLSTEEEI
                     RRDILPSEIIGGGRPTTLGVRDMLEKGDINFQDAKHRVVQTFEREFFMEAVRMNNWNI
                     SQTANKLGMKRQYLQQKLKELELNIQEAKENI"
     gene            3123..4007
                     /gene="murI"
                     /locus_tag="PK754_12535"
     CDS             3123..4007
                     /gene="murI"
                     /locus_tag="PK754_12535"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNB10378.1"
                     /translation="MMRSQTPEPSVLPAIQSAPPASEQPIGIFDSGIGGLTVVKEVMN
                     QLPYERIIYLGDTARVPYGTKSDRTIKSFTLQSCLFLLEHNVKMIIIACNTASAVALD
                     FLSNMFKIPVIGVIVPGSEAAVRKSLRKKIGVIGTHTTVNSQAYKKTIHALDAQCEVF
                     SQPCSLFVPLAEEGWLRHDVTQRIAAEYLNVFSDKDIDTLVMGCTHYPILSGAIQEAM
                     NTIMRRPVTLIDSGVETAKRTRQILDEKKLLNTSGQKPYHRFYVTDLPKNFMRVGEMF
                     LGSKIDSVETVNVEVMGF"
     gene            complement(4079..4480)
                     /locus_tag="PK754_12540"
     CDS             complement(4079..4480)
                     /locus_tag="PK754_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10379.1"
                     /translation="MKTNFLTSLHPKILYAGLVLSSLIGYLEWGSGHYTFLVQAEFEI
                     IRKFFTHTDAITHPMIALPLLGQALLLVTLFQNKPRPVIVLTGLLGLGVLLWFIFLIG
                     LISLNLKITFAALPFLIVSIIVLYSMRKGGK"
     gene            complement(4446..>4870)
                     /locus_tag="PK754_12545"
     CDS             complement(4446..>4870)
                     /locus_tag="PK754_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10380.1"
                     /translation="LVIHTSPENIWRVWMAVEEWSLWTDTILSIKKLSPGSLQTGSRV
                     LIKQPKLAKAVWKITEFEPVKQFTWSKINFFLRIDAIHALRSTTDGCIVTLVIKFSGL
                     FANMINKKTCALVDSYLVRESQNLKQFCENKFSNVAAS"
BASE COUNT         1344 a         1062 c         1124 g         1240 t
ORIGIN      
        1 tgagggcatg agttttatac cgccttatgt tttttggctg atcgtatgtt tattagtttc
       61 tggtttttta tcgtcacgca ttatacgtct cagccgcaac gaaaaatggc gtcaattctt
      121 tatcgtcgaa cttatcatca cgatcgtcat ctccgtgctt ttggtatatt tctattttta
      181 tggcggtaca ggcaacgtgc gtctggatcg gtcgctcaaa aacatgatta tgatttcgtc
      241 gttgataccg acgggtgcga tggtgtatct gctgtacaaa tatccttttt attcgacggt
      301 cgcaaggcgc cggtttttat tgatcaccat ttcgggtgtt tttctcgtag tattcattat
      361 gagctccaaa gcgcttcgca aaatggcgga ggaaaatccc gaattaaacg tcgaatttct
      421 tgagatcgtg ctcgtatcca tgttgtttct cgtatacgaa ccgaccaaat atcttgtacg
      481 ccgcatcgcg gggcaatttg cgttgcatga aaaatcgtat ttccaatcgt tgattcgtaa
      541 gatcacggag catatcgtca gtgccgccaa tctcgccgaa ctgacaaaag ccgttcgtac
      601 cggtatcgtg gatgcactgc atgtcgaaga agtcgggtta tacacgttga ataaaaccgt
      661 atccggagag cgaacgctat acagtatcgt gcatgcatac ggtgatatca aaacatttaa
      721 tctcgaaaag attacgcgcc atattcaaaa aggaaacggt ctgtatgatg cgcgaaagat
      781 gaacattttt caccttcgca gcgccgaagt accgtatcaa atttatctcg ggattgtttt
      841 ggataacgaa ctggtaggga tcttggcgat cggaaaaaag aagagcaatg aggattttga
      901 attcgaagaa aaagaattat tacttacagt agcgagtcag gttgcgattt ctatcgaaaa
      961 catacagctt ctccaacgtc gtatcgaact tgaatccaaa atgtttgaag cggacaaact
     1021 gacctcgctg ggtatgctgt ccaccagtat tgcgcacgaa gtgaaaaatc cgctaagctc
     1081 catcaaaagc atcgtgcaat ccatgcacga tcagaaaaaa aaggaaaaca ccgacacgga
     1141 agcgattcag gatttggaaa ttattaacga agaaattgat cgcttgtcgt ccgttgtcaa
     1201 tcagcttctg cgttttgcac gaagcgacag ttcgggcatg gatcgtattg atattatcaa
     1261 aatcatagat gccatcctta cgatctcacg tcaggaaacg cgggttcgca atatccgcat
     1321 ttttacgcgt tatgaccatg caccgctcgt cattcgctca cgccaaggcg acttgaaaga
     1381 aatcatattt aaccttatta tcaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnttgggc gaaattttca aaccattttt tacaaccaaa agcgaaggta ccggtttggg
     1561 tctcgctata gtcaaaaata aaatacaagc gctcggaggt cgtctcgttt tacgtagcga
     1621 actccaggta ggaacgtcgt ttgaagtgta tattcccagc gaaagtacat cccgatcatg
     1681 agtgacatac cggtcgaatc ttctcaagag cacacatcgc atatccgcat tctcgtcgtg
     1741 gatgatgaaa aagcggcgcg ttatggtatg ctcaaagcgc tcaaacccga aggttacaaa
     1801 ttatcggaag ccgacgacgg attatcggcg ctcaatctca tggccaataa tccctttgat
     1861 atcgtcatta cggatgtatc catggccggc atgaacggta tggatttgct gaaaaaaata
     1921 aaagaacgca cgccgcattg ttttgttatc gtgatcacgg cgcacgggtc ggaacgtctt
     1981 gccgtcgagg ctatcaaatc cggcgcgttt aattatttgg ccaaacccta cgatatcgaa
     2041 gaactgcggg cactcgtgtc gcatgcggca caacaaatcg ccgtcgtgca tgaaaacgaa
     2101 cgattacgcg ccgagctcaa agaagccaaa ggtttcgggc aatttgtcgg cagcagcaaa
     2161 cccatgcgtc aggtgtttga tttgttggaa cgggtaagcc gaaataacat tacggtactg
     2221 atacgcggcg aaagcggcac cggaaaagag ttagtcgccc gcacgataca tgaaaaaagc
     2281 aatcgccgta gcggcccgtt tatttcgatc aattgcgccg cactgcctaa agaacttatt
     2341 gaatcggagc ttttcggtta cgaaaaaggt gcatttaccg gagccatatc ttccaaacaa
     2401 gggcgctttg aattggccga tggcggcaca ttatttttgg atgaaatcgg cgatatgtcg
     2461 cttgaaacgc aggccaaagt tttgcgtgtc atccaggagc gtaagtttga acgcgtcggc
     2521 ggtaccgaat ccatcagcgt ggatgtacgc attctaagcg caacgcaccg tgacctggcg
     2581 cgatttatcc gcgacggtca gtttcgcgaa gatttgtatt atcgtatcaa tgttgttgag
     2641 atcgaattgc ccccgctgcg gtcccgctcg gaggatattc atcttttggt gaatcactac
     2701 cttgtgatgt ttgcgcaaaa acataaagcg caggtgacgc gtttttcgca agacgcgctc
     2761 aaagcgatgc tcgaatacga ttggcccggc aatgtacgcg aattgatcaa cgtggtagag
     2821 cgttgtgtcg tgctcagtac cgaagaagaa atccggcgcg atattttacc ttcggagatt
     2881 atcggtggag gacgaccgac gacacttggc gtacgcgata tgctggaaaa aggcgatatc
     2941 aattttcagg atgccaagca tcgcgtcgtg cagacgtttg agcgcgaatt ttttatggaa
     3001 gcggttcgca tgaataactg gaacatttcg caaacggcga acaaactcgg catgaaacgg
     3061 caatatcttc agcaaaagct caaagaactg gaactgaaca tccaggaagc gaaagaaaat
     3121 atatgatgcg ttcacaaaca ccggaacctt cggttttacc ggccatccaa tcggcgccac
     3181 cggcgtcgga acaaccgatt ggaattttcg attcgggcat cggcggactt actgtcgtca
     3241 aagaggtaat gaaccaactg ccgtatgaac gcattattta tctcggtgat acggcgcgcg
     3301 taccctacgg aaccaaatcc gatcgtacga tcaaatcgtt tacattgcaa agctgccttt
     3361 ttttgcttga gcataatgtc aaaatgatca tcatcgcatg caatacggcc agcgccgtgg
     3421 cgctcgactt tttatccaat atgttcaaaa tacccgtcat cggcgtgatc gttccggggt
     3481 cggaagcggc cgtgcgaaaa agtctgcgga aaaaaatcgg tgttattgga acgcatacta
     3541 cggtcaattc gcaagcctat aaaaaaacca ttcacgcgtt ggatgcccaa tgtgaggtgt
     3601 tttctcagcc gtgttcgtta tttgtaccgc tggccgaaga aggttggctt cgtcatgatg
     3661 tgacacaacg catcgccgcg gaatatttga atgttttttc cgataaagac attgatacgc
     3721 tcgtcatggg gtgtacgcat tacccgatat tgagcggtgc gatacaagag gcgatgaata
     3781 cgatcatgcg ccgtccggtc actttgattg atagcggtgt cgaaacagcc aaaagaaccc
     3841 gacagatatt agacgaaaaa aagctgctca acacatccgg gcaaaaaccc tatcaccgct
     3901 tttatgtgac ggatcttcct aagaacttta tgcgtgtggg cgaaatgttt ctaggatcga
     3961 aaatagacag cgtcgaaacc gtcaatgttg aagtgatggg tttttgatat agcgccgccg
     4021 ttttccaacg catcgtaaaa tttcacgtag cgaagttttt tactacgatt tctcaacgtc
     4081 atttcccgcc tttacgcatc gaatacaaaa caatgatact gacgatcaga aaaggaagtg
     4141 cagcgaaggt tatttttaga ttgagagaaa tcaaaccaat caaaaatata aaccacagca
     4201 aaacgcctaa ccccagtaaa ccggtcaaca ctatgacagg acgtggttta ttctgaaaca
     4261 gggtaacaag caacaatgct tgccctaata atggaagcgc aatcatcgga tgagtgatcg
     4321 cgtcggtatg cgtgaaaaac ttacggatga tttcaaactc tgcttgcacg aggaaagtgt
     4381 agtgaccgct accccattcg agataaccga tcagtgaaga gagtaccagc cccgcgtaca
     4441 gtattttagg atgcagcgac gttagaaaat ttgttttcac agaattgttt caaattttgt
     4501 gattccctta ccagatacga atcaaccaaa gcgcaggttt ttttgttaat catattagca
     4561 aaaagaccgg agaatttgat aaccaatgtg acgatacatc cgtcggttgt tgatcgcaaa
     4621 gcgtggattg cgtctatacg cagaaagaaa tttattttac tccacgtaaa ttgtttcacg
     4681 ggttcaaatt cggtaatctt ccaaaccgct ttggcaagtt taggttgctt gatgagcacc
     4741 ctgctgcctg tttgtaaaga acccggcgat agttttttta tggataggat cgtgtcggtc
     4801 cagagagacc actcttctac agccatccat acgcgccaga tattttccgg cgacgtatga
     4861 atgactaagg
//
LOCUS       DAOQNQ010000243         4854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_7381_length_4854_cov_5.885810, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000243 DAOQNQ010000000
VERSION     DAOQNQ010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7381_length_4854_cov_5.885810"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PK754_12550"
     CDS             complement(<1..347)
                     /locus_tag="PK754_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10381.1"
                     /translation="MKIMKFFHEERRRPMLLIGLIAFTLRLGLQEVYGVDVMPSDGLD
                     YHNVAVNLVLGHGYSKDTQALHNPYFFREPGYPIFLSAIYFLYNLFDPVSYLDSAQHV
                     LNDHNEILWARFV"
     gene            659..1555
                     /gene="gnd"
                     /locus_tag="PK754_12555"
     CDS             659..1555
                     /gene="gnd"
                     /locus_tag="PK754_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HNB10382.1"
                     /translation="MQLGFIGLGKMGGFMVQRLMRDKHEIVGYDLNPDVVKGLTEKGM
                     TGASSYEDLVGKLKGRKAVWIMVPAGKPTEDTVAKLANILDKGDIIIDGGNSYYKDDV
                     RRAKELEAKGICYVDVGTSGGVWGLQNGYCMMVGGNKADVDFLSPILTTLAPPNNGWA
                     HMGAHGSGHMVKMVHNGIEYGMMQAYAEGFEIMKASEYKLDIARIADLWMQGSVVRSW
                     LLELTAEGLKKNPTLDGIKGWVADSGEGRWTILEAMEKDVPAPIITLSLFERFHSRQP
                     ESFAAKMLAMMRNQFGGHEVKK"
     gene            1633..2151
                     /locus_tag="PK754_12560"
     CDS             1633..2151
                     /locus_tag="PK754_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB10383.1"
                     /translation="MDKVIIVRAVPEDAEKLTHISRQTFYETFSEQNTKENMQKYLDE
                     SFSIERLTDEMHDAHSQFYFAKSGHDIVGYIKINLRPSSAEIKNIYSLEIERIYVRHD
                     FQGKKVGLSLYEKAMHIARESKADHVWLGVWEKNYNAITFYNKLGFVAFGSHPFRLGD
                     DIQTDILMKKQL"
     gene            2212..2775
                     /locus_tag="PK754_12565"
     CDS             2212..2775
                     /locus_tag="PK754_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HNB10384.1"
                     /translation="MNAFKKISKTQCTSLIETLKIRFEKNRIRHTAIKWTDVSVKIEN
                     KTTKLFSLWKMEETGGEPDVIGFDIKTGEYIFCDCAPESPAGRRSLCYDKDALDARKE
                     HKPKDNAMDVAARMGIDILTEEAYRELQKLGEFDTKTSSWIKTPDQIRKLGGALFCDR
                     RYDHVFVYHNGAESYYAARGFRGMLKI"
     gene            2920..3564
                     /locus_tag="PK754_12570"
     CDS             2920..3564
                     /locus_tag="PK754_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10385.1"
                     /translation="MIETENAQTYVLKMRGSGNGAISLVSEFIVSRIASQLGWPIPDA
                     QWIQIPDNLPWTFGSDEFDDIVQKSFGWNLGISYIPDSFPITFDNQLLTDPVLVSALY
                     TIDMFFMNMDRTNSANNLLRDIHNKIWIIDHGALALFQPGVKQTLGLFHNHILFEIPS
                     ISKKQYNTALHTPELFKTIIHQIPDAIINETHFSKETLLARIINRTVAMSEFVV"
     gene            3958..>4854
                     /locus_tag="PK754_12575"
     CDS             3958..>4854
                     /locus_tag="PK754_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB10386.1"
                     /translation="MRNLKITYKLLIGFGTLLAMLIGLSYFAISKMSDLFGMSKAAID
                     VIPAQNAIVEIQYHTTKGHLWFEEMLSGDNSIRMEEVYDHWNGGIEYASVLLDGGTRD
                     NQTYSAVKDTALIEILTSIRYALVDMVNAADERLELKKQKIKSEAGTESDIYFDAAYQ
                     RVMDYCDNGKKITTGLINKSVRNLENDFSSSRWTLIIETTVIVLIGILFSTLIARTIA
                     LPVREAALKINRDGVNTRFDMQRKDEIGQLVQTVDSFLSSFRMILREVADASAAVSSS
                     SKQIGVSTENMARNAQDQMGQTA"
BASE COUNT         1524 a         1006 c         1009 g         1315 t
ORIGIN      
        1 tgtacgaaac gcgcccaaag aatttcattg tgatcattca aaacatgttg agccgagtcc
       61 aaatacgata cgggatcgaa taaattatat aaaaaataaa tggccgacag aaaaatcgga
      121 tacccgggtt cacgaaagaa ataaggattg tgcaaggcct gagtatcttt cgaatatcca
      181 tgacccaaca ctaagttcac ggcgacatta tggtaatcca atccatcgct aggcatcaca
      241 tccacaccat acacctcctg taagccaagg cgtagcgtga aagcgattag tcctatcaac
      301 agcataggcc tgcgacgttc ttcatggaag aacttcataa ttttcatatc aagagggttg
      361 aaaagaaata acgagaacca acttcagttc ttcaagttag agcgattttg aaaaaatgca
      421 acaaatgttt gtatagcgct gccattcatc gccaatcata ctattttatt tttcgggtga
      481 atgattggaa cgaaatttgt tcatagcacg aatatttaaa aaatagttta ttcgataccg
      541 ttcataaaac attttgcatt tatctgcact tttgatcaaa ttcatgcgcc tttccacaac
      601 tactgaaatc attcgttgaa cgttttatag tccaccaatt ttttaccgga ggtattttat
      661 gcagctcgga tttatcggcc ttggaaaaat gggcggtttt atggtacagc gcctgatgcg
      721 cgataaacat gaaatcgtcg gttacgacct caatcccgat gtcgtgaaag gtcttacaga
      781 aaaaggaatg accggcgctt cttcctatga agaccttgtg ggaaaactca aaggccgtaa
      841 agcggtatgg atcatggtcc ctgcgggtaa gccaacagaa gataccgtcg ccaaactggc
      901 taatattctc gacaaaggag acatcatcat tgacggtggt aattcgtact ataaagacga
      961 tgtacgccgt gccaaggaac tcgaagccaa aggtatttgt tatgtggatg ttggtaccag
     1021 cggcggcgta tggggcctgc aaaacggtta ctgcatgatg gtcggcggta ataaagccga
     1081 tgtggatttc ctttcaccga tcctgacgac acttgcaccc cccaataacg gttgggcgca
     1141 catgggcgca catggcagcg gtcacatggt caaaatggtt cacaatggaa tcgaatacgg
     1201 tatgatgcaa gcttatgccg aaggttttga aattatgaaa gcatccgaat ataagctgga
     1261 tattgcacgc atcgccgatc tgtggatgca aggttcagtg gtgcgctcat ggcttctcga
     1321 acttaccgcc gaagggctca aaaaaaatcc gacgctcgac ggtatcaaag gttgggtggc
     1381 cgactccggc gaaggccgat ggactatcct cgaagcgatg gagaaagacg tacctgcacc
     1441 catcattaca ctgtctcttt tcgaacgttt ccactctcgt caaccggaaa gttttgcagc
     1501 taaaatgctc gctatgatgc gcaatcaatt cggcggtcac gaagtaaaga aatagttttt
     1561 taatacggcg cgttacccaa agcctattcg tccaacggat aggctttgtt attcaacttc
     1621 aaaaacgtat tcatggataa agtgattatt gtccgagcgg taccggagga cgcagaaaaa
     1681 ctgacacata tcagtcgtca aacgttttat gagacttttt cagagcaaaa cacaaaagaa
     1741 aacatgcaga aatacctgga cgaatcgttt tctatcgaac gtcttaccga tgaaatgcat
     1801 gatgcccatt cgcagtttta ttttgccaaa tcaggacacg atatcgtcgg ttatataaaa
     1861 ataaatctcc gcccatcatc agccgaaata aaaaatatct attcgttaga aatcgaaaga
     1921 atttatgtgc gacacgattt tcaaggaaaa aaagtcggac tttccctgta cgaaaaagca
     1981 atgcatatag cacgagaatc aaaagcagat catgtttggt tgggcgtgtg ggaaaagaat
     2041 tataacgcta ttacatttta taataagctc ggatttgtcg cgtttggctc gcatcccttt
     2101 cgtttgggtg atgatataca aaccgatatt ctgatgaaga agcaacttta gctgtctgtt
     2161 aaataattta ttctcaaact aatttcggtt taccatataa cacgtaatat catgaacgct
     2221 tttaaaaaaa tatccaaaac acaatgcacc tccttgattg aaactttaaa gatcagattt
     2281 gaaaaaaaca gaattcgcca tactgcaatc aagtggacag atgtatctgt aaaaatagaa
     2341 aacaaaacaa ccaagttgtt ttccctctgg aagatggagg aaaccggagg tgaaccggat
     2401 gtcataggct ttgatataaa aacaggcgaa tatattttct gtgattgcgc accggaaagc
     2461 cccgccggac gtcgaagtct atgctatgat aaagacgcat tagacgcccg caaagaacac
     2521 aaacctaaag acaacgcgat ggatgtggct gcacgcatgg gcatagacat cttaaccgaa
     2581 gaagcataca gggaattaca aaaattaggt gaattcgaca ctaaaacatc cagctggata
     2641 aaaacacccg atcaaatcag aaaacttggc ggtgcactat tctgcgatcg tcgttacgac
     2701 catgtttttg tctaccacaa cggcgccgaa tcgtattacg ctgcacgcgg ttttcgggga
     2761 atgctaaaaa tttgaggtca gattactttt tttagttttg tagaaaacat tgccacggcg
     2821 cccgtcaagc ctgttgttgg attatttatc ttaacatgac aaagaatttt gaaacttaaa
     2881 cgatttattc aacaatatag aggcagctca ttcccaacct tgattgaaac ggaaaacgcg
     2941 cagacttacg tcctgaaaat gcgcggttcc ggtaacggcg ctatcagcct tgtttctgaa
     3001 tttattgtat cccgcattgc atcacaattg ggttggccca tacccgatgc acaatggata
     3061 caaattccgg ataacttacc ttggacgttt ggcagcgatg aatttgacga catcgttcag
     3121 aaaagtttcg gctggaatct tggaattagc tatataccgg actcttttcc gatcacgttt
     3181 gataatcaat tattaacgga tccggtttta gtatccgctc tgtatacgat tgacatgttt
     3241 tttatgaata tggacagaac caatagcgcc aataatctgc ttcgggatat ccataataaa
     3301 atatggatca ttgatcatgg cgcattggct ttgtttcaac cgggagtaaa acaaactctt
     3361 gggcttttcc ataaccatat tctctttgaa ataccttcaa tttccaaaaa acaatacaac
     3421 accgcattac atacacccga actcttcaag acgattattc atcaaatacc tgacgccatt
     3481 atcaatgaaa cacatttcag caaagaaact ttgctggctc gtatcataaa cagaaccgtc
     3541 gcgatgtctg aattcgttgt ataaattcta gcctcattga tgtatgacct gcttaacgat
     3601 ttatgcaaat aaaagatcat tgcgaatctt tgccttcctc acaggatgtg actaacaatc
     3661 ctatttgcat ctttaacgct gattcggtaa gtttacacgc atttctttgg ataccggtta
     3721 aattttcggt tgtttgcttg cttagacaca aaacttcgtc aaaaattatt ccaaagacga
     3781 aagttacagt attctcataa ttttatagtt aaaatgcatt cgttttgatg cattaccaaa
     3841 ataactatgc gatacatatc gcgaatcaag tgtagaaata aaacataaac agcaattcgc
     3901 aagcttttat aattacataa aaaataataa agacaatata tatccctacg agggcttatg
     3961 agaaacctca aaatcacgta taaactactc atcggttttg gcacgttgct cgccatgtta
     4021 atcggcttat cctattttgc gatcagcaaa atgtccgatt tgtttggcat gtccaaagca
     4081 gcaatagatg tgatacctgc gcaaaacgct attgtcgaaa ttcaatatca tacgaccaag
     4141 gggcaccttt ggtttgagga aatgttgtcc ggtgataatt cgattcgtat ggaggaagta
     4201 tatgatcact ggaatggcgg catcgaatat gcatctgtat tattggatgg cgggactcgt
     4261 gataatcaaa cgtattccgc tgtcaaagac acggctttga ttgagatact cacaagcata
     4321 cggtatgcat tggtggatat ggtcaatgcg gcggatgaac gcttagaact taaaaaacaa
     4381 aaaataaaat cagaagccgg tacggagtcg gacatctatt ttgatgcagc ttatcagcgt
     4441 gtcatggatt actgcgacaa cggaaaaaaa atcacgaccg gacttatcaa taaaagtgtc
     4501 cgcaatcttg aaaatgattt ttcaagttcc cgatggacat tgattataga aaccacggtt
     4561 attgttttga tcggcatttt gtttagtacg ctcattgccc ggactatcgc tttgccggtg
     4621 cgtgaagcgg cgctcaagat caaccgcgat ggtgtgaata cccggtttga catgcaacgt
     4681 aaggatgaga tcggacaatt agtacaaacc gtggatagtt tcttaagttc attccgcatg
     4741 atcttgcgcg aagtagccga tgcgagcgcc gctgtatctt catccagtaa acagatcggc
     4801 gtgagtacgg aaaacatggc gcgcaatgca caagatcaaa tgggccaaac agcc
//
LOCUS       DAOQNQ010000244         4831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_7445_length_4831_cov_5.559464, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000244 DAOQNQ010000000
VERSION     DAOQNQ010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7445_length_4831_cov_5.559464"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..2378
                     /locus_tag="PK754_12580"
     CDS             81..2378
                     /locus_tag="PK754_12580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNB10387.1"
                     /translation="MIVRYSLFLLCAAGVLYSQNYFKDTEALTFYDQDGMIPYASHGG
                     MYVPKTEFADIDSDGDPDLFIVQTDGRITHYMNSGVEGPLRWLWITDNFASLEVNQWV
                     RFIDADADGDLDLFCGVSGARMGYYKNEGSAVNAHFVLQTETVLDTLNAPVLTEDQTV
                     PVFKDIDSDGDYDFFSGRSVGTLSYYENVGNEHSFLLKFITDAFQDIAIITPAKQSEE
                     QRHGASAVCFFDIDDDRDPDLFWGDFFSTGLYFLPNLGDSAHPFIPDTATSTYPSRQL
                     KTLGYNMPSFVDADGDGDGDLLVSVLYREEDRDNLWYYRNIGTPQAAQFQLITKNLLQ
                     NIDVGRNAHPIMADLDNDGRLDIILGSYSGAIHFYRQTVNGVFQSNSSWFVTIPQDEF
                     ITAPALGDIDNDGDLDLFVGKFDGKIMFYRNTGSLTSPEFTLENQFYANLDIGNNSSP
                     FLSDFEGDGDLDLIIGEESGEVQLISNNGTLSNPDFSTATVTLIAANGLSDAVPILLD
                     WDGDSDRDLIVGYKNGTLRYYERQNANSFQEIAGKFSTIKVTGQAVPFALDWDNDGDK
                     DIVCGNLRGGIEFYRAGTQKPIIKIPNKQKIRSDSSWTYRLIANANPAPRWQILSGPS
                     SLKCDSISGIVQWKPVVTDIGTHVVEVRAYNIDGADTAKFEINVSVYEESPLQLLYNS
                     PNPFDERTSIVMKINKTMEVKAYIYDVRGGLVHRLVSGQLSPGIYKREWNGKNTHGNK
                     VASGIYFVTVVANGKTYSRKILFIK"
     gene            2485..2664
                     /gene="rpsU"
                     /locus_tag="PK754_12585"
     CDS             2485..2664
                     /gene="rpsU"
                     /locus_tag="PK754_12585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNB10388.1"
                     /translation="MLSTIVRKNEPIEKAIRRFETEVRKARIIQTCIEKSNYVSPSER
                     KHIAHKRKKRNTGNA"
     gene            2685..4016
                     /locus_tag="PK754_12590"
     CDS             2685..4016
                     /locus_tag="PK754_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB10389.1"
                     /translation="MSQIIIIGNGITGTTVARHVRKRSDQPITIISGESDYFFSRTAL
                     MYIYMGHMKYEHTKPYEDDFWKKNEIQLRRGLVERIVPAEKQIYLKNGEAMKYDKLVI
                     ATGSVYQTFGWPGQNLQGVQGLFSLQDLELMERNTKSVKRAVIVGGGLIGIEMAEMLI
                     SRGIHVTFLVREKSYWDNILPAEESDMINRHIRAHHVDLRLNTELQEIRGNDKGRVDA
                     IVTKSGEVIPCEFVGLTVGVKPNIEFLRSSGIPLNRGVLINQFLETPIADIYAAGDCA
                     EFIEPKPAHPKIEQLWYTGRMQGEALARVLTGERQAYDRGIWFNSAKFFDIEYQTYGV
                     VPNVPRDGEEHLWWEHPDGQHGIRIVFRKDNRIVIGFNLMGIRYRQNVCQRWIKESRT
                     IDYVLQNLGEANCDPEFFMQYEPHIIAEYNRKYPEQNLLLQVQRGLHFIVR"
     gene            4046..>4831
                     /locus_tag="PK754_12595"
     CDS             4046..>4831
                     /locus_tag="PK754_12595"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNB10390.1"
                     /translation="MNQPVPIQIPLVTLSLHQKISLALVVIGFLMLGVALSPISEQYP
                     IGMFLLSSGLITIGALAYIMPMWYDRKPGIKHDRIFFNSFVNRGVWGWIAAVFFTGFY
                     VVLYWFPERLEYLIHTTDWLSGILRNKPADQWFLYGFYYTLAVMVMGLRMMAKNRHSR
                     YQLIRTVSVIFFQLGFAFLLPGLLQKFNQPEFYFSYFWPLKYSYLFPNDINWLISQPG
                     GLGTFMVFWGAVMSFIATPILTYFFGKRWYCSWVCGCGGLAETL"
BASE COUNT         1357 a          946 c         1124 g         1404 t
ORIGIN      
        1 gtgatatttt tacctatatt gctgttagct tgtttccgtg agatcagaca ccgtttcttt
       61 aagtagctta ttatctcatt atgatcgtta gatattctct atttcttcta tgtgcggcgg
      121 gcgtacttta ctcgcaaaat tatttcaaag ataccgaagc gttgacattc tacgatcagg
      181 atggcatgat cccatacgca tcgcacggcg ggatgtatgt acctaagacg gagtttgcag
      241 atattgattc ggatggtgat ccggatttgt tcatcgtaca aaccgatgga cgtataacgc
      301 attacatgaa tagcggcgtg gaagggcctt tgcggtggct atggataacg gataattttg
      361 ccagtttaga agtgaatcaa tgggtacgtt ttattgatgc cgatgcggat ggggatttgg
      421 atttattttg tggggtttcc ggtgcacgta tgggttacta taagaatgaa gggagcgcgg
      481 taaatgcaca ttttgtgttg caaacggaaa ccgtgttgga taccctcaat gcacccgtct
      541 taacggaaga ccagactgta cccgttttta aagacattga ctctgatggt gattatgatt
      601 ttttttctgg tagaagtgtc ggaacactgt cgtattatga aaatgtgggg aacgaacaca
      661 gttttttatt aaaatttatt actgatgcgt ttcaagatat tgcgatcatc acccctgcta
      721 agcaaagcga agagcaaaga catggtgcaa gtgctgtttg tttttttgat atagatgatg
      781 atcgggatcc ggatttattt tggggcgatt tcttcagtac gggtttgtat tttttaccaa
      841 accttggcga ttcggcgcat ccttttattc cggataccgc tacatctact tacccatcgc
      901 ggcagttaaa aacattagga tacaacatgc cgtcatttgt agacgccgat ggtgacggcg
      961 acggtgatct tttggtaagc gtattatacc gtgaagaaga ccgggataat ttgtggtatt
     1021 atagaaacat aggaacaccg caagcggccc aatttcagtt aatcacaaag aatctattac
     1081 aaaacattga tgtcggtcgt aatgcccacc ccataatggc tgatctggat aacgacggcc
     1141 ggttggatat tatccttggt agctatagcg gggcgatcca tttttatcgt cagacagtca
     1201 acggggtttt ccaaagcaat tcttcatggt ttgtcacaat accacaagat gaatttatta
     1261 cggcacctgc tttaggagat atagataatg acggcgattt ggatttattt gtcggaaaat
     1321 tcgacggtaa aataatgttt taccgcaata ccggcagttt aacgagtccg gaattcacat
     1381 tagagaatca attttatgca aatttggaca taggcaataa tagctcgcca tttttatcag
     1441 attttgaggg cgacggcgat ctggatttga ttattggaga agagagcgga gaagttcaac
     1501 tcatatctaa taacggcact ctgtcaaatc ctgatttttc tacggcaacc gttacattaa
     1561 tcgctgcaaa cggtctttcc gatgccgtcc ccatattatt ggattgggat ggtgattcgg
     1621 atcgtgattt gatcgtaggc tacaaaaatg ggacgctacg ttattatgag cggcagaatg
     1681 caaactcatt tcaggagatc gcaggaaagt tttcgactat taaagtcaca ggtcaagctg
     1741 tgccgtttgc tttagattgg gataatgacg gcgataaaga tatagtgtgc ggtaatttgc
     1801 gaggaggtat cgaattctat agagccggta cacaaaaacc aatcataaaa attccaaaca
     1861 aacaaaaaat tcgatcggat tcatcatgga cgtatcgtct tatcgccaac gctaatcctg
     1921 cgccgcgttg gcaaatatta tcagggccgt catcactgaa gtgcgattcg atttccggta
     1981 tagtgcagtg gaaaccggtg gtgacggata taggcactca tgtcgtagaa gtgcgtgcct
     2041 acaacataga cggcgccgat acagctaaat ttgaaatcaa tgtttcagtg tacgaagaaa
     2101 gcccgctcca actactttac aatagcccca acccatttga tgaacgaacc agcatcgtta
     2161 tgaaaataaa taaaacgatg gaagtgaaag cgtatatata cgatgtgcgg ggtggtttgg
     2221 tgcatagatt agttagcggc caactttcgc cagggatata taagcgtgaa tggaatggaa
     2281 aaaatacgca cggtaataaa gtagccagcg gtatatattt cgtaacggtc gtagctaacg
     2341 ggaaaaccta ctctcgaaag attttgttta tcaaataaca gggaataaaa actcacagtg
     2401 taagttacat gaagcaaccg gaaactttgg ccgggtgttt gcgtatatgc ggtatatcct
     2461 tctatctaac ctgtgaggtg taccatgtta tcaacgattg ttcggaagaa cgaacccatt
     2521 gagaaagcta tccgtcgttt tgaaacagaa gtgcgcaagg cgcggattat tcaaacatgt
     2581 atcgaaaaat caaactatgt cagccctagt gaacgaaaac atattgccca taaacgaaaa
     2641 aagcgcaata cgggcaacgc ataacccgtt ttaataaatc cattatgtcc caaattatta
     2701 ttatcggtaa tggtataacc ggtactaccg tagcccgcca tgtccgtaaa cgcagcgatc
     2761 aaccgatcac tataatctcc ggtgaaagtg attatttttt ttcccgtacg gcactgatgt
     2821 atatctatat ggggcacatg aagtacgagc acactaagcc ctatgaagat gatttttgga
     2881 aaaaaaatga aatccaactc aggcgtggat tagtcgaacg catcgtccct gccgaaaaac
     2941 agatttacct gaaaaacggc gaggccatga agtacgataa attagttatt gccaccggat
     3001 ctgtttatca aacattcggc tggccgggtc aaaatcttca aggtgtgcag ggactatttt
     3061 ctttgcaaga tcttgaactg atggagcgaa acaccaagtc ggtaaagcgt gctgtgatcg
     3121 taggaggtgg attgatcggt atcgagatgg ccgaaatgct gatctctcgc ggcattcacg
     3181 tgactttcct tgtacgagaa aaatcttact gggataatat tttgccggcg gaagaatcgg
     3241 atatgattaa tcgccacatc cgtgcgcatc atgtagattt gcgattgaat accgaacttc
     3301 aggagattcg cggtaatgat aaaggtcgcg tggatgcgat tgtgactaag tccggagaag
     3361 ttataccgtg tgagtttgtc ggtttgacgg tcggtgtaaa acctaatatt gaatttttac
     3421 gttcttccgg aattccgctg aatcgcggtg tactgatcaa tcaatttttg gaaacgccga
     3481 ttgctgatat ttatgccgcc ggtgattgtg cggagttcat agaacccaag ccagcgcatc
     3541 ccaaaattga acaactttgg tacacgggtc gcatgcaagg tgaagcgttg gcgcgtgttt
     3601 tgacgggtga acgccaagct tatgatcgag ggatttggtt taactcggct aaattttttg
     3661 acatcgaata tcagacatat ggtgtagtgc caaacgtacc ccgtgacggc gaagagcatc
     3721 tctggtggga acatcctgac ggacagcatg gtatccgaat tgtttttcga aaagataacc
     3781 ggattgtaat cggatttaat ctgatgggga tacgctacag acaaaatgta tgtcaacgtt
     3841 ggataaaaga aagccgaacg atcgactatg ttcttcaaaa tttgggcgaa gctaattgcg
     3901 atcccgaatt ttttatgcag tacgaaccgc atatcattgc tgaatacaac cgaaagtatc
     3961 ctgagcaaaa tcttcttttg caggtccagc gcggcctcca cttcattgta cgataaatta
     4021 ttaaatctta catagatcat cccctatgaa tcaacccgtc ccgattcaga taccccttgt
     4081 tacactaagt ttgcaccaaa agatatctct cgctttagtt gttataggtt ttttgatgct
     4141 tggcgttgct ctaagtccga tatccgaaca atatccaatc gggatgtttt tactttccag
     4201 cgggcttata accataggag cattagccta catcatgccc atgtggtatg acagaaaacc
     4261 cggtataaaa cacgatcgaa ttttttttaa ttccttcgtc aatcgcgggg tttggggatg
     4321 gattgccgcc gtatttttta ccggatttta tgtggtactt tactggtttc ctgagcgact
     4381 agaatacctg atacacacga cagattggtt gagcggcatt ctcagaaata aacccgctga
     4441 tcaatggttt ctttacggat tctattatac gctggcagtg atggtaatgg gtttgcgtat
     4501 gatggccaaa aacagacata gccgttacca actgatccga acggtttcag taatattttt
     4561 tcagcttggt tttgcatttc tcttacccgg gttgctccaa aaattcaatc aacccgagtt
     4621 ttatttttca tacttctggc ctcttaaata cagctatttg tttcccaacg acatcaattg
     4681 gttaatatcc caacccggag ggcttggtac atttatggtg ttttggggcg ccgtgatgag
     4741 ttttattgca acgcccatac ttacatattt tttcggtaaa cggtggtatt gttcatgggt
     4801 ttgcggctgt ggcggtttag cggagactct g
//
LOCUS       DAOQNQ010000245         4810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_7493_length_4810_cov_5.908097, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000245 DAOQNQ010000000
VERSION     DAOQNQ010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7493_length_4810_cov_5.908097"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="PK754_12600"
     CDS             <1..1317
                     /locus_tag="PK754_12600"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10391.1"
                     /translation="RRFVGQGPTERPLTGTYYPADMTKEEFEAYIKANPKEEKELTGL
                     YTVVVRDGKKLKAVPFHEVYKAEVTAISQLLAQAAALCENVSLKTYLTERAKAVLTDD
                     YYASDVAWMKLKDNNIDVVIGPIENYEDRLYNYKTAHECAVMVKDPEGTKELQMFQSH
                     LDALEKNLPSKKEYIRSSAGKANVLEVVNIVYFGGDFQKGVKTIAASLPNDPKVHDEY
                     GGKKQMYKNLMEAKFQKILLPLGERLIAPDSRKYIDMHSFTTFVTMHEVSHTLGRSFV
                     YGKDDLPVRKALKELYSAIEEAKADVVGIYNMDYFAKQGIYDAETMRKHYTTYVVGLF
                     RSIRFGAEEAHGKANIAQLAFLQEQGALGRDAQGYWQIHPEKFQAGLSALAKVLLEIE
                     AEGNYDGAKTFIEKYGKLTDAIKADLAKFHDIPRDLNTTYDIMNQL"
     gene            1349..2218
                     /locus_tag="PK754_12605"
     CDS             1349..2218
                     /locus_tag="PK754_12605"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNB10392.1"
                     /translation="MPITWINGKYVPEDEIALSPNDRGFLYGDGAFETMRSVQSRIIA
                     LHAHIHRLKTACERLRIPLTQGPEDFRTIIQELLNRNALQDAYIRISISRGPVEQFGF
                     GFSKTIKPTCLIAVRPAPTIPDNIYQKGISVGFQISSLFGSHTGDTAIKSLSAVEYVL
                     AKQSALENKCYDMLLMDSIERVYEGTSSNVFIVRQGALCTPPVHSGILPGITRARVIT
                     LAETKLHLPVREILFSKQDVLTAEEVFLTNTNIDVLPVTVAGEQTIGNGVPGKITQSL
                     LQLYRQTLIEILE"
     gene            2211..3614
                     /gene="pabB"
                     /locus_tag="PK754_12610"
     CDS             2211..3614
                     /gene="pabB"
                     /locus_tag="PK754_12610"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HNB10393.1"
                     /translation="MNNESAVHIEPWDWPDDIWSLYRTIAASPRFIFLYSSLLQPSYG
                     RYSYLACDPSIVLKCTSHHNEITTATGVTSIDGNPFDCMYDLMQQYAAPKNHANVDFC
                     TDGWFGYWGYDIRDHLEKIASRARKSLNTPDALWMKFDAVIILDHQEHQAWITGTRTG
                     IEKLRERLQQPVASYPEKPLQFKIHPTETRASYIQKINSLHESIAAGDVYEVNLSQRF
                     DVTVDGYDPNVYSRIFRSLVETSPAPFSAIIQCDETAVISSSPELFLQMHQNICTSKP
                     IKGTRPRTGNFDSDEKNYFDLQTDEKDRAENLMIVDLVRNDLGRVSQAGSVVTKDLFA
                     VETYATVFQMVSTIQSRLSEHATMMDAVKAAFPPGSMTGAPKISAMQHIESLEPYKRS
                     VYAGAIGWMQASGETMLSVVIRTLIFNGASGYFQTGGAIVWDSIPDEEYQECWDKAQG
                     ILRALEKLGVINLPVYK"
     gene            complement(3722..4741)
                     /locus_tag="PK754_12615"
     CDS             complement(3722..4741)
                     /locus_tag="PK754_12615"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNB10394.1"
                     /translation="MKTLLRTMVIIAFGLLLSCSSRIRQAAYPTLFDNEYDSEFPYRS
                     SSDQLEEISASVKIMFSTAFYNSYYFDEKFAITPTAVRAGAAEKYAYRYLNTQASVSG
                     TATVIYYDTKRVALLTCAHVVEFSDTVYSYYPSGHVQSVSFLQRQSNFIRDLPEGGEL
                     EVLAIDSKNDIAIIGRSFDHIPQEPVHVFGYPIGQSKKLRWGSFVYVIGYPMGFKMVT
                     KGTVSSPNRDRNGSFLTDAPMNEGLSGGIVLAIKDGVPNFELVGVAKSISATQEYYLM
                     PDPGFTQEIPAPSLYKGELYARLKTEYRYGITHVVSTEILRSMYEKYKKSIDAKGYSM
                     DALFK"
BASE COUNT         1354 a         1146 c         1071 g         1239 t
ORIGIN      
        1 cgtcgctttg tcgggcaagg gcccaccgaa cgtccgttga cgggaacgta ttatcccgcc
       61 gacatgacca aagaagaatt tgaagcgtat atcaaggcca accctaaaga agaaaaagaa
      121 ctgaccggat tgtataccgt agtcgtgcga gatgggaaaa aactcaaagc cgttcctttt
      181 catgaagtgt acaaagcgga agttacggcc ataagccaat tgcttgcgca agccgctgca
      241 ttgtgcgaaa atgtctccct caaaacatat ctcaccgagc gcgccaaagc cgtattgacg
      301 gacgactact acgcatccga tgtcgcgtgg atgaaactta aagataacaa catagacgtc
      361 gtgatcggcc ccatcgaaaa ctacgaagat cgtctgtaca actacaaaac agcacatgaa
      421 tgcgccgtga tggtcaaaga tcccgaaggc accaaagaac tgcaaatgtt ccagtcgcac
      481 ttggatgcgc ttgaaaaaaa cctcccgtct aaaaaagaat acattcgctc ctcggccggc
      541 aaagccaatg tgcttgaggt ggtcaatatt gtttacttcg gcggcgactt tcaaaaaggc
      601 gtaaaaacga ttgctgcatc tttacccaac gatccgaaag tccatgatga atacggcggc
      661 aaaaaacaga tgtataaaaa tctgatggaa gctaagtttc aaaaaattct gcttcctctc
      721 ggcgaacgtc ttatcgcacc ggattcacgt aaatatatag acatgcattc gtttacgaca
      781 ttcgtaacga tgcacgaagt gtcgcacacg ctcggacgca gttttgtcta tggtaaagac
      841 gatctgcctg ttcgtaaagc tttgaaagaa ttgtattctg ccatcgaaga ggccaaagcc
      901 gacgttgtcg gtatttacaa tatggattat tttgccaaac aaggcattta cgatgcggaa
      961 accatgcgca aacattatac aacctacgtt gtcggtttgt tccgttcgat ccgatttggc
     1021 gccgaagaag cgcatggtaa agccaacatc gcgcagttag catttttgca agaacaaggt
     1081 gcgttgggac gtgatgcgca aggttattgg caaattcatc ccgaaaaatt tcaagccggt
     1141 ctgtcggctt tagctaaagt actgcttgaa atcgaagccg aaggaaacta tgacggagct
     1201 aagacgttta tcgaaaaata cggtaaactg accgatgcga tcaaagccga cttggccaag
     1261 tttcacgata ttccgcgcga tctcaatacg acatacgata taatgaatca actttgatcc
     1321 ggtcacttta taaaccgatg gcggaatcat gcccataacc tggatcaacg gcaaatatgt
     1381 gcccgaagat gaaatcgctt tgtcgcccaa cgaccgtggt tttttatacg gcgacggggc
     1441 gtttgaaacg atgcggtccg tgcaatcgcg catcatcgca ttgcatgcac atatacaccg
     1501 tttgaaaacg gcctgtgaac gattgcgaat tccgcttaca caaggacctg aagatttccg
     1561 aacaatcatt caagaattgc tcaaccgaaa cgcgctgcaa gatgcttata tacggatcag
     1621 tataagccgc ggcccggttg agcaatttgg ttttggattt tctaaaacca tcaaaccgac
     1681 ctgtctcatc gccgtacgcc ctgcaccgac gattcctgat aatatttacc aaaaaggcat
     1741 atctgtcgga tttcagatca gttcgctttt tggttcacac accggtgata cggccatcaa
     1801 atcattgagc gcggtggaat acgttcttgc caaacaatcg gcgcttgaga ataaatgcta
     1861 cgatatgtta ttgatggatt cgatcgaacg cgtgtacgag ggcacctcca gtaatgtatt
     1921 tatcgtccgt caaggtgcgt tgtgcacgcc gccggttcat agcggtatac ttccgggtat
     1981 tacgcgcgca cgggtcatca ctttggctga aacaaaactt catctgcccg tgcgggaaat
     2041 cttgttttct aaacaagatg tactgaccgc agaagaggtt tttttgacga ataccaatat
     2101 tgatgtattg ccggtaaccg tcgccggtga acaaaccata ggcaatggtg taccgggaaa
     2161 aatcacacag tcgttacttc aactgtaccg ccaaactctg atcgaaatcc ttgaataacg
     2221 aatccgccgt acatatcgaa ccttgggatt ggccggacga tatttggtca ctgtatcgca
     2281 cgatcgccgc ctctccgcga tttatttttt tatacagctc gttgctacaa ccatcgtacg
     2341 gccgatattc gtaccttgca tgcgaccctt ccatcgtttt aaaatgtacc tctcatcaca
     2401 acgagattac tacggctacc ggcgtgacat ctatcgacgg gaaccctttc gattgcatgt
     2461 acgatctgat gcaacaatat gccgcgccaa aaaatcacgc caacgttgat ttttgtacag
     2521 acggctggtt tggatattgg ggttatgata ttcgcgatca tctggaaaaa atcgcatcac
     2581 gcgcccgcaa gagtttgaat acgcccgatg cgttatggat gaaatttgac gccgttatca
     2641 ttttagatca ccaagagcat caggcgtgga ttaccggtac tcgtaccggc attgagaaat
     2701 tacgcgagcg ccttcaacag cctgtagcct cgtatccaga aaaaccgctt caatttaaaa
     2761 ttcatccgac cgaaacccgt gcatcctaca ttcaaaaaat taactccctg catgaatcaa
     2821 tcgctgcggg cgatgtgtac gaagtcaatc tatcgcagcg ttttgatgtg acggtagatg
     2881 gctacgatcc gaacgtgtac agtcgtattt ttagatcgtt ggtcgaaaca agtcccgcgc
     2941 ctttttcggc gattattcaa tgcgatgaaa cagctgtaat cagttcatca cctgagctct
     3001 ttttgcaaat gcatcaaaat atatgcacca gcaaaccgat caaaggcaca cggccacgca
     3061 cgggtaattt tgattccgac gaaaaaaatt atttcgattt acaaaccgat gaaaaagatc
     3121 gcgccgaaaa tctcatgatc gttgatctcg tccgcaatga tcttgggcgc gtttcacagg
     3181 cgggctctgt tgttacgaaa gatctttttg ctgtcgaaac gtacgccacc gtttttcaga
     3241 tggtttctac gatacagagc cgattgtccg aacatgctac gatgatggat gccgtgaaag
     3301 cagcatttcc acccggttcg atgacgggcg cccccaaaat aagcgccatg caacatatcg
     3361 aatcgttaga accttataag cgcagtgttt atgccggcgc tatcggctgg atgcaagctt
     3421 cgggggaaac aatgttatcg gtggtaattc gaacccttat tttcaacgga gcctccggtt
     3481 attttcaaac cggcggggcc atcgtgtggg attctatacc cgatgaggag tatcaggagt
     3541 gttgggataa agctcaggga attctgcgcg cgcttgaaaa attgggagta attaacttac
     3601 cggtatataa ataacctata cgtcatccta tcgcccatcg tcacatcatc tgcaatgcct
     3661 agccgaaccg gttttttaga ttcttcaaaa tttcatttct attttttgtc agcaaaagcg
     3721 gctatttgaa aagcgcatcc atcgagtaac ctttggcatc aatgcttttt ttgtattttt
     3781 cgtacattga acgcaaaatt tctgttgata cgacatgggt gatgccgtaa cggtattctg
     3841 ttttaaggcg agcgtacagt tcacccttat ataaacttgg cgcaggaatt tcctgtgtaa
     3901 aaccgggatc cggcatcaga taatattcct gcgtagcgga tatggatttc gccacgccca
     3961 cgagttcaaa attgggcacc ccgtctttga tggcgagcac gatccctccg ctgagtcctt
     4021 cattcatcgg cgcatcggtc agaaaggaac cgttacgatc gcgattggga ctgctcactg
     4081 tgcctttggt taccatttta aaacccatcg gataaccgat cacgtacaca aatgaacccc
     4141 atcgtaattt tttggattgc ccgatcggat atccgaaaac atgcacgggt tcctgtggaa
     4201 tatgatcaaa tgaacggccg atgatcgcga tatcattttt gctatctatg gctaaaactt
     4261 ccaactctcc gccttccggc aaatcgcgta taaaattgct ttgacgttga agaaaagaca
     4321 ccgactggac atgcccgctc ggataataag aatataccgt atccgaaaat tcgacaacat
     4381 gtgcacaagt aagtaaagcc acacgtttgg tatcataata aatcaccgtt gccgtaccgg
     4441 acacagaggc ttgcgtatta agataacggt acgcgtattt ttctgccgca ccggcgcgca
     4501 ccgccgtcgg agttatggca aatttttcat caaaatagta cgaattataa aacgccgtgg
     4561 agaacatgat ttttaccgaa gcgctgatct cttccaactg atccgaactg cttcggtatg
     4621 ggaattcgct gtcgtattca ttatcaaata aggtcggata agccgcttga cgaattcgcg
     4681 aagaacacga aagcaagagg ccaaatgcga taatgaccat cgtacgcaat aatgttttca
     4741 taaaatttcc ccgatttagt ttaacgatga acctttctgc atctctaccg gctttacacg
     4801 agataagtta
//
LOCUS       DAOQNQ010000246         4778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_7562_length_4778_cov_5.036629, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000246 DAOQNQ010000000
VERSION     DAOQNQ010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7562_length_4778_cov_5.036629"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PK754_12620"
     CDS             <1..401
                     /locus_tag="PK754_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10395.1"
                     /translation="ITEWNSFTAGGGYLDDPKQIVIGKKNLFIKDRTGVKIFDIFGNY
                     IRTINDTFFSANLLLSADEQDRVLIWDDDKKSLTIWNAEGQRVTLTIAFAFENPTAMT
                     CFGHSLWIVDQSSTVGESKLYQIEITEESR"
     gene            402..3941
                     /locus_tag="PK754_12625"
     CDS             402..3941
                     /locus_tag="PK754_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10396.1"
                     /translation="MLYRFAVIFLSAIVIDLSVASAQDETPPYISADSSIQHTLLSKN
                     INYFQGAHTIRGYSSDTLYYLPFRRIIPESEIFTIGYQKLTREKDYTIRYSDGVVRFS
                     FVVDSSITVECRFLVSRLGIDDSYARRDLPIIRTNIISSKTDTIPRWTPPNPSSEFTN
                     DLNGRGTITRGFTIGNNQDFTLNSGLNVQVTGQIGEDVTLEAVITDEQTPIQPEGNTE
                     RLQEIDKIYIEVRKADKFTATLGDFEIQTTGTQFGNFHRSLQGVRSLYNDGRTDAAAA
                     YGTAKGKYHSNTLTITEGVQGPYELTGKNGERNPIIVAGSERVWLNGVALTRGENNDY
                     VIDYNAGQITFTRKRLVTPESRIIVDFEYSNEVFRRNTASASVRSQFFDRRLTVGGAF
                     LSESDDKNNPLNLSIDQQLRDSLASLDDRGLQGNGNTILVDGGRWVGEGRGAYYKVYD
                     ANSNDSIYVYSGSDSSGSYNVRFTDVGSGRGDYIRGNILGEFIYVGSGLGYYLPLVPM
                     DLPTRLRTGIWYAAWNPVQNLRLRTEFALSQFDKNLYSPRQSQGYAYQLEAFVADQDI
                     RLFKTKLGKFDLQIIRRNQDSNFTAPDRTQDAEYGRQWNIFNPATSVYSVTAPGLQET
                     VNDFRLAYKPWKGVQTDFFKGELTRGDHQFVSTKSGSGLKIDRKNIFVSGSIHDIQIT
                     DRTQSVIVNNHTQRKFFDFNGAWRIWRPGLYYEDESQKNRQSDSVYGASYQVIRPNLN
                     IITGQYLTLGASYEMRSDRIRNDAVDSLDGRVSKSINQRYYWQWTEWHNLNHTFEWQR
                     RKREYFGSKKTIANPDRISNLVNASADYHPFSRAVQVTVDYQISQEQLQNRKIVFIPV
                     PPNTGNFVQVRQDSFRQVPQGQGDWIQSAVRGQGFTPVVELKSGIRLRLEIGRLRSRS
                     SKDSISDRWQSRFIERLIYEGLFRIEENQTDPDRRFYWLQAVQNKNTQRGNLLLRQEL
                     SGYDRIYDMLWRLRYETNRTRNRLLNDGQDKNQRDIYYLRIQKQVNSFWSWEQEGEVT
                     IAQRQSLINVSSLNIRYDIHKNRYSPSIVFKPKPTMEYHLRTDWLLAKDYETGSRAKI
                     MNWQPTMVFSWLNRGRWTNTLELTRLWLKDGLGNTPFEVTDGRVRGWNWLWISAVDYR
                     AGEHVIMRANYTGRKYPGQKIIHLGTAEVQAFF"
     gene            complement(3953..>4778)
                     /locus_tag="PK754_12630"
     CDS             complement(3953..>4778)
                     /locus_tag="PK754_12630"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNB10397.1"
                     /translation="PIQVCRDYHIPRWVNKESLVIACSYSGNTEETIAALHEAMEAQA
                     QILTIATGGTVQSIANQHGLSHITIPGGLQPRQAIGYALTTLHAVFSEIFKSAPVREE
                     MSHIVFQMKHLATILKTVHDNNPYIHDSKILHDKPVIIYASERMFPAALRLKGQITEN
                     AKLLAFAYAVPEMNHNEIVGWESVHEAHHAPCHVILLRDREDHPQVQKRFEILMRLLK
                     GKAHWTEYTSDGGTLMERYYNLIYRGDWLSYYMAIARNTDPTPVDIITRLKNELAS"
BASE COUNT         1361 a         1007 c         1123 g         1287 t
ORIGIN      
        1 ctataacgga atggaatagt tttactgcgg gcggcggtta tcttgacgat ccaaaacaga
       61 tcgttatcgg taaaaaaaat ctatttatca aagatcgaac cggtgtcaaa attttcgata
      121 tttttggaaa ttatatccga accataaacg atacattttt ttcggcaaac ttacttctat
      181 cggctgatga acaggatcgt gtgttgatat gggatgacga caaaaaatcg ttaacgatat
      241 ggaatgccga agggcagaga gtgactttga caatagcttt tgcatttgaa aatcctaccg
      301 caatgacttg tttcggtcac tcgctttgga tagtagacca gtcatcgact gtcggggaat
      361 caaagctcta tcaaatcgaa attaccgagg aatcgcgata gatgctttat cgttttgccg
      421 ttatatttct atcggccatt gttatcgatt tatccgtggc gagtgcacag gatgagacgc
      481 ctccttacat ttcggcagac tcctcaatac agcacacctt gctatccaaa aatattaact
      541 attttcaagg cgcacacacg attcgcggat attcttccga tacattgtac tatcttccgt
      601 ttcgccgcat catacccgaa agtgaaattt ttactatcgg atatcagaaa ttaacccgcg
      661 aaaaagatta tacgatacgt tattccgatg gtgtcgttcg tttttcattt gtcgtagatt
      721 cttcgattac ggttgagtgc cgttttttag tttcccgatt aggcattgat gattcgtatg
      781 cccgccgtga tctgccgatt attcgaacaa atatcatttc atccaaaacg gataccatac
      841 cgcgatggac accgccgaat ccgtcatcgg agtttaccaa cgatttgaat ggccgtggta
      901 caattacgcg cggatttacg atcggcaaca atcaggattt tactctaaat tccggattga
      961 atgtgcaggt tacaggccaa atcggggaag atgttacgct ggaagcggta ataacagacg
     1021 aacaaacgcc aatccagccc gagggaaata ccgaacgcct tcaggaaatc gataaaatct
     1081 atatcgaagt acgtaaagcg gataaattta ccgcaaccct cggtgacttt gaaatacaaa
     1141 caacaggcac ccaatttggc aattttcacc gttcgcttca aggcgttcgt agtttgtaca
     1201 acgatggacg taccgacgcg gctgcagctt acggaacggc aaaaggcaaa taccacagta
     1261 atacgctaac cataaccgaa ggcgttcaag ggccttacga attaaccgga aaaaacggtg
     1321 agcgaaaccc gattatcgtt gccggtagcg aaagggtgtg gcttaatggt gtagccttga
     1381 ctcgagggga aaataatgat tatgtgatag attataatgc aggtcagatc acgtttacac
     1441 gaaaacgttt ggtcacacca gaatcaagaa tcatcgtcga ttttgaatat tccaatgaag
     1501 tgtttagacg taatacagct tcagcatcgg tacgttcaca attttttgac cgacgtttaa
     1561 ctgtaggagg cgcatttcta tcagagtccg atgataagaa taatccgctt aatctgtcta
     1621 ttgaccaaca gttacgcgat tcgctcgcat cgttggatga tcgggggcta caaggcaatg
     1681 ggaatacgat actggtagat ggaggacgat gggtcgggga agggcgcggc gcttattaca
     1741 aagtctatga tgctaattcg aacgatagca tttacgttta ttccggatcg gacagctccg
     1801 gctcgtacaa tgtgcgtttt accgatgtcg ggtctggacg cggtgattac atccgcggta
     1861 atattttggg tgaatttatc tatgtcggat cgggtttggg ttattattta ccgctcgtac
     1921 cgatggatct gcctacacgg ctgagaacgg gaatatggta tgcggcatgg aacccggttc
     1981 agaatctgcg gttgcgaaca gaattcgcat tgagtcaatt tgataaaaat ctttattccc
     2041 cacgccaatc gcaaggttat gcctaccaat tggaagcttt cgttgcagat caggacattc
     2101 gtttatttaa aacgaaactt ggaaaatttg atttacaaat tatacgccgt aatcaggata
     2161 gcaattttac tgcgcccgat cgcacacaag atgcggaata cggtcgtcaa tggaatatct
     2221 tcaatccagc gacgtccgta tattcggtaa ccgcgcccgg attacaagaa accgtgaatg
     2281 atttcagatt ggcatataag ccgtggaaag gcgtacaaac tgattttttt aagggtgaat
     2341 taacgcgcgg cgatcatcaa tttgtatcca ctaaatccgg gtcgggctta aaaatagatc
     2401 ggaaaaatat tttcgtatcg ggcagcattc atgatataca gattacggat cggacacagt
     2461 ccgtcatagt gaataaccat acacaaagaa aattctttga tttcaatggc gcgtggcgga
     2521 tatggcggcc gggtctgtat tacgaagacg aatcgcaaaa aaacagacaa tcggattcgg
     2581 tttacggcgc ttcgtatcag gtgattcgtc ccaatttaaa catcattacc ggtcaatatc
     2641 ttacccttgg cgcaagttat gaaatgcgat cggaccgcat tcgaaacgat gccgtggatt
     2701 ccctcgatgg aagggtatca aaatctataa accaacgtta ttattggcaa tggacggaat
     2761 ggcataatct caatcacacg ttcgaatggc aacgaagaaa aagagaatat tttggaagta
     2821 agaaaacgat agccaatccg gatcgcatca gtaatctggt aaacgcaagc gccgattatc
     2881 atcctttttc cagagccgtg caagttaccg ttgattatca gatatctcag gagcaattgc
     2941 agaaccgaaa aatcgttttt attccggtgc cgcccaatac aggcaatttt gttcaagtgc
     3001 gtcaagatag tttcaggcag gttccgcaag ggcaaggcga ttggatccaa agcgccgttc
     3061 gcggtcaagg atttactcct gtggtggaac taaaatctgg aattcggcta cgtttggaaa
     3121 ttggtcgctt gcggagtcgt tcttcaaaag actctataag tgatagatgg caatcccgat
     3181 ttatagaacg tttgatttat gaaggtcttt ttcgcatcga agaaaatcaa acagatcccg
     3241 atcgtcgctt ttattggctc caagcggttc aaaacaaaaa tacacaacgg gggaatctcc
     3301 ttttgcggca agagttgagc ggctacgatc ggatctacga tatgctctgg cgattgcgtt
     3361 atgaaaccaa tcgtacacgt aatcgtcttt tgaatgacgg acaggataag aatcaacgcg
     3421 atatctatta tttacgcatt caaaaacaag tgaattcgtt ttggtcatgg gagcaagaag
     3481 gagaagtcac gatcgcccaa agacaatctt tgatcaatgt aagcagcctc aatatccggt
     3541 atgacattca taaaaacaga tactcgccaa gtatcgtatt caaaccaaaa ccgacgatgg
     3601 agtatcattt gcgaaccgat tggctgttgg cgaaggacta tgaaaccgga agtcgcgcta
     3661 aaatcatgaa ttggcagccg acgatggttt tttcatggct caaccgcggg cgatggacta
     3721 ataccctcga actgacacgc ctttggttga aagacggatt aggtaatact ccgtttgaag
     3781 tcaccgatgg ccgtgtacga ggatggaatt ggttatggat ttccgccgtg gattataggg
     3841 ccggcgaaca cgtcatcatg cgggcaaatt ataccgggcg aaaatacccg gggcaaaaga
     3901 tcattcatct cggaacggcc gaagtgcagg cgttttttta ataaatgcta atctaagacg
     3961 cgagttcgtt tttcaaccgc gtgatgatat ctaccggtgt ggggtccgta tttcgggcaa
     4021 tcgccatata gtagctgagc caatcgccac gatatatgag attataatag cgctccatca
     4081 aggtcccgcc gtcggacgtg tattccgtcc aatgggcttt ccctttgagc aatcgcatca
     4141 atatttcaaa gcgtttttgc acctgcggat gatcttcgcg atctcttaat aagatcacat
     4201 gacagggagc atgatgggct tcgtgtacgc tttcccaacc gacgatttca ttgtgattca
     4261 tttcgggaac ggcataagca aaggctaata gtttagcatt ttccgtgatt tgccctttga
     4321 gtcgcaatgc cgcaggaaac atacgttccg acgcatagat gattaccggc ttgtcatgca
     4381 gtattttgct gtcgtgaata tagggattat tatcgtggac ggttttgagg atggttgcga
     4441 ggtgcttcat ttgaaaaacg atgtgagaca tctcttcacg aaccggggcg gatttgaata
     4501 tttcgctgaa aaccgcatgg agtgtcgtaa gtgcataacc gatggcttgg cgtggctgca
     4561 aaccacccgg tatagtgata tgcgataatc catgttgatt tgcgatggac tgaaccgtac
     4621 caccggttgc aatggtaaga atctgcgctt gcgcttccat ggcttcgtgt aaggccgcaa
     4681 tggtttcttc cgtattgcct gaataactgc acgcaataac taaactttct ttattgaccc
     4741 aacgcggaat atgataatca cggcaaactt gaatcggc
//
LOCUS       DAOQNQ010000247         4765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_7602_length_4765_cov_5.323992, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000247 DAOQNQ010000000
VERSION     DAOQNQ010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7602_length_4765_cov_5.323992"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PK754_12635"
     CDS             <1..408
                     /locus_tag="PK754_12635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB10398.1"
                     /translation="EEGTNLRAWLFKILVNTHISQYRKNAKDPATTGYDDVEEFSLYS
                     QVRSRAAFPDDAPTDILNQFLDKDIQAAIQALPQQFKDVVMLVEIEGFSYQEAANILD
                     VPVGTIMSRLFRGRKLLQKSLWEYAVEYGYVKK"
     gene            454..753
                     /locus_tag="PK754_12640"
     CDS             454..753
                     /locus_tag="PK754_12640"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNB10399.1"
                     /translation="MSHVHHGEAGLHAQNHPINCGEIERLLYAYLDGELPLEEVAPYK
                     DHLSHCPPCKAFVEFEEKISHLIKQKCGHNGTDRACVPTSLHEKIQKAIALSKEV"
     gene            complement(809..2440)
                     /locus_tag="PK754_12645"
     CDS             complement(809..2440)
                     /locus_tag="PK754_12645"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNB10400.1"
                     /translation="MKVILAQINSRVGDLTNNTERIIQHINQARSEGADLIVFPELSI
                     TGYPPEDLVEKKAFVRKNQEMLDRIREATDGVGVLCGFVAPNNDPEGHHVFNAAALIE
                     NRQIIGIQYKTLLPNYDVFDEMRHFEPAKHKKIFTFRGVKLGISICEDSWNDKDFWKR
                     RIYHNDPIEELFNQGAEILINISASPFHVQKHHLRLEMFRSIAVKYRKPILFVNLVGG
                     NDSLIFDGRSFALNAEGIPIATGKAFEEDRVSLTLPSTQTITMPNPTTEEEIFNALVL
                     GVRDYLHKCGFRKALIGLSGGIDSALVAAITASALGPQNVIGVSMPSRYSSDHSQDDA
                     RDLARNLDITYHSVPIEPMFRSFLESLSGSFQGLPEDTTEENIQARIRGNILMAFSNK
                     LNAMVLSTGNKSELAVGYCTIYGDMCGGLAVISDVPKTMVYRISNWINRDKIIIPKST
                     IEKPPSAELRPNQTDQDSLPPYDVLDAILKAYIEDLLEADEIIKLGHDPETVHKILKL
                     IDRNEYKRRQAAPGLRVTSKSFGFGRRLPIAQGWR"
     gene            complement(2620..3033)
                     /gene="lexA"
                     /locus_tag="PK754_12650"
     CDS             complement(2620..3033)
                     /gene="lexA"
                     /locus_tag="PK754_12650"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNB10401.1"
                     /translation="MEPKIIYDNQQNPGIPVVGKVAAGTPRFSEEISLGHVVSSRYSP
                     EDKKLFALQIVGDSMIEDGIFNGNYIVCRQTNEFVNGDIVIAYVNEEATVKRIYKRGS
                     KIILQPANAELSPIEVDPQYQSFRVGGKVLEVLRH"
     gene            complement(3036..>4765)
                     /locus_tag="PK754_12655"
     CDS             complement(3036..>4765)
                     /locus_tag="PK754_12655"
                     /inference="COORDINATES: protein motif:HMM:NF015655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10402.1"
                     /translation="NTLKGLKQLFSQKLLSPNEFQTLSSAYIFYRKIEHILQIEKDTQ
                     KHTLSGTTDEFTKIAILLGYTNGSTLESVLNENLKSVRAIYLSVFEIDNRATSVFSGL
                     ISEVCSEEGIRFLQEHHFTDPDRTHRTIRLLAMGQFPKLYGNSVRECLAELLEILLTD
                     VGQTHDPDLTLAHLERIVAAQPFADMLYRSLSHNAEYRKHLVYLCSLAPGLTQIFCER
                     PSYLDWLQIQLPTWLSTPHQGAQAIYRLTKNLQHIRRLGWVQCALTNRNQPISSSEPY
                     LYLSELADQILQTAFSEVFAMKEPIILLGMGKLGGSELSYRSDLDVVWVCSDDADVDH
                     FISQARRVHQRITDHASEGKIYDMDARLRPEGAQAPLVVTLSRYKEYFSSRALFWEVQ
                     SLIKARCIAGSSDLHSQFESWRYEQLRTASRKWNIRHEITTMRERQIREKIKSPADAV
                     YEIKTCRGGLLDIEYLVQGLQLLHGDRLPQSPLQSAVQAINSLISAGLLLVDDASILT
                     TNYYLLRRIEEAAFLLFERKSSRLPSDEKQLAFIANFLDFKNNDAFLMRLEQIKESNH
                     RLFLHYLGV"
BASE COUNT         1309 a         1016 c         1143 g         1297 t
ORIGIN      
        1 gaagaaggta ccaatcttcg cgcttggctt tttaagattt tagtcaatac gcatatatcg
       61 caataccgta aaaatgcaaa agaccctgcg acgacgggtt acgatgatgt cgaagagttt
      121 tcattgtata gccaagtaag aagccgcgct gcttttccgg atgatgcgcc gacggatatt
      181 ttaaatcagt ttttagataa agatatccag gcggcgatac aggcgttacc tcagcaattt
      241 aaagatgtgg taatgctggt tgagatcgaa ggtttttcgt atcaggaagc ggcgaatatt
      301 ttagatgtac ctgtcggtac gattatgtca cgattattca gagggcgtaa gctgctgcaa
      361 aaatctctat gggaatacgc agtggaatac ggatatgtga agaaataaat agtaaagcgg
      421 agttatgaat ttcggaggag tggaggagag gttatgagtc atgtacatca tggcgaggcg
      481 ggtctgcatg cgcagaacca tccgatcaat tgcggtgaaa ttgaacgact tttgtatgct
      541 tatttggatg gtgagttgcc attggaagaa gttgcgcctt acaaagatca tctatcgcat
      601 tgcccgcctt gcaaagcatt tgtcgaattt gaagaaaaaa tcagccatct catcaagcaa
      661 aaatgcggtc acaacggtac agatcgcgct tgtgtgccga catcgcttca tgaaaaaata
      721 caaaaggcga ttgcattgtc caaagaagtt tgatgtgtga tagactgata ataagagata
      781 aaatataacg gctaatgtta atactacttt accgccaacc ttgagcaata ggtaatcgcc
      841 tcccaaaacc gaacgactta gaagttacgc gcaaacccgg tgcagcctgc ctgcgtttat
      901 attcatttcg atcaataagt ttgagaattt tgtgaaccgt ctccgggtcg tggccaagct
      961 ttataatctc atcggcctct aaaagatctt cgatataggc tttcagaatg gcatccagta
     1021 cgtcataagg cggaagcgaa tcctgatcgg tttgattggg gcgcaattcg gcagaaggcg
     1081 gtttttcgat ggtacttttg ggaataatta ttttatcgcg attgatccaa tttgaaatgc
     1141 gataaaccat cgtcttgggc acatcactga tcacggctaa accgccgcac atatcgccat
     1201 agatcgtgca gtatccgaca gccaattcgg atttgttgcc ggtgctaagt accatggcgt
     1261 tcaatttatt tgaaaacgcc atcaagatgt tgccgcgaat tcgggcttgg atattttctt
     1321 ccgtcgtatc ctcgggcaaa ccttggaagc taccggataa actctccaag aaactgcgga
     1381 acatgggttc gataggaacg ctatggtacg tgatgtcgag attccgggcc aggtcgcgag
     1441 cgtcatcttg gctgtgatcg gaggaatatc gggacggcat ggatacgccg atcacattct
     1501 gcgggcccaa ggcactcgcg gtgatcgcag caaccaacgc cgaatcaata ccgccggaca
     1561 atccgatcaa ggctttgcgg aatccgcatt tgtggaggta atcccgtacg ccgagcacga
     1621 gggcattaaa aatttcttct tcggttgtcg gattgggcat agtgatcgtt tgcgttgaag
     1681 gaagggtgag agaaacccga tcttcctcaa aggcctttcc ggtcgcgatt ggtattcctt
     1741 ccgcattgag cgcaaaacta cgcccgtcaa atatcaagct gtcattgccg ccgacaagat
     1801 tgacgaaaag tatcggttta cggtatttaa cagcgatgct gcgaaacatt tccaaacgca
     1861 aatgatgttt ttgtacatgg aaaggtgacg cggaaatatt gatcagtatt tctgcacctt
     1921 gattgaaaag ttcttcgatc gggtcattgt gataaatacg tcgcttccaa aaatccttgt
     1981 cattccaact gtcttcgcaa atggagatgc cgagtttgac accgcgaaaa gtaaatattt
     2041 ttttatgttt tgccggttca aaatgacgca tttcgtcaaa tacatcatag ttgggcagta
     2101 gcgttttgta ttgtatgccg ataatctggc ggttttcgat cagagccgct gcgttaaata
     2161 catgatgacc ttccggatca ttattgggcg cgacaaagcc gcacaaaacg ccgacgccgt
     2221 cggtggcttc cctgatccga tccaacatct cctgattttt tctgacaaaa gcttttttct
     2281 caacgagatc ctccggagga taacccgtaa tgctcaattc aggaaatacg atcagatcag
     2341 cgccttcgga gcgcgcctga ttgatgtgtt ggataatccg ttccgtatta ttggtgagat
     2401 cgccgacgcg ggaattgatt tgagctaaaa tgactttcat gtattaacta tgatcgttta
     2461 ggcttatcaa taaaaaaggt cactcgaaaa tgcgtccagc ttgtctgaaa aatgagaatc
     2521 tgcgtgggaa atataaaatc ggcacaacag atgcgtgatt cagtatgcta tagatgacgc
     2581 tttttggagc aacctttgtg tgaatttctt aatcggttat caatgccgta aaacttcgag
     2641 aactttgccg ccgacacgaa aagattgata ttgcggatca acttcgatcg gcgaaagttc
     2701 ggcattggcc ggttgtaaaa taattttact tccgcgtttg taaatacgtt ttaccgtagc
     2761 ttcttcattg acgtaggcaa taacgatgtc accattgaca aattcgttag tctgacggca
     2821 tacgatgtag tttccgttaa aaataccgtc ttcgatcatc gagtctccaa caatctggag
     2881 tgcaaaaagt tttttatcct ccggcgaata acgggaagaa acaacatggc cgagcgaaat
     2941 ctcttcacta aagcgcggtg ttccggcagc cactttaccg acgacgggaa tgcccggatt
     3001 ttgctgatta tcgtaaataa tcttcggttc catatttata ctcccaaata atggagaaat
     3061 aaacgatgat tggattcttt gatttgttca agccgcatta gaaatgcgtc gttgttttta
     3121 aagtcgagaa aattggcgat aaaggcaagc tgtttttcat cggaaggcaa acgcgaagac
     3181 ttgcgttcga atagtaaaaa agccgcttct tcgatgcgtc gtaaaagata atagttggtt
     3241 gtaagtatag acgcatcgtc aaccagaagc aggcctgccg atatcaggct gttgatggct
     3301 tgtacggcgc tttgcaacgg tgattgcggc aaacgatcgc cgtggagcaa ttgtaatcct
     3361 tgcacgaggt attcgatgtc caacaatccg ccgcggcatg ttttgatttc atacaccgca
     3421 tcggccggag atttgatttt ttcgcgtatt tgacgttcac gcatagtggt tatttcatgg
     3481 cgaatattcc attttcttga ggccgtcctt agttgctcat aacgccacga ttcgaattga
     3541 gagtgaagat cggacgaacc ggcaatacag cgcgccttga tcagcgactg gacctcccaa
     3601 aacaaggcac ggcttgaaaa atattcctta tatcgcgaca gcgtaacgac aagtggcgct
     3661 tgggcgcctt ccgggcgcaa acgggcatcc atatcataaa ttttcccctc ggacgcgtgg
     3721 tcggtgatgc gctgatgaac gcggcgtgct tgagaaataa aatgatctac atccgcgtca
     3781 tccgaacaca cccaaacgac atccaaatcg gatcggtagc tcagttcact tccgcccaac
     3841 tttcccatac ctaagagaat aattggttct ttcatcgcga acacttcact gaaggcggtt
     3901 tgtaaaattt ggtctgcgag ctccgaaaga tataaatagg gctctgacga cgaaatcggt
     3961 tggttgcgat ttgtgagggc gcattgaacc cacccaagac ggcgaatgtg ctgaagattt
     4021 tttgtcaatc ggtaaatggc ttgtgcgccc tgatgaggcg ttgataacca tgtcgggagc
     4081 tgtatctgca gccaatccaa ataggaaggt ctttcacaaa aaatctgtgt caatcccggc
     4141 gctaacgaac agagataaac aaggtgtttt cgatattcgg cattatgcga caacgagcga
     4201 tatagcatat cggcaaaagg ttgcgccgcg acgatgcgtt caagatgtgc cagcgtcaaa
     4261 tccggatcgt gcgtctgacc gacatcagtc agtaagattt ccagcagttc cgcaaggcat
     4321 tcacgaactg agttgccgta taatttggga aactgtccca tagccaaaag gcgaatcgta
     4381 cgatgcgttc gatccggatc ggtgaaatga tgttcctgca aaaaacgaat tccttcttcc
     4441 gaacatacct ctgaaattaa tcccgaaaat actgaggttg cacggttatc aatttcaaat
     4501 acagatagat aaatcgcgcg aacagatttc aaattttcat ttaaaactga ttctaatgtc
     4561 gaaccgttcg tatagcctag tagaatcgcg attttggtga attcatcggt tgtcccgctc
     4621 agggtgtgct tctgggtgtc tttttcgatc tgaagtatgt gctcgatttt tcgataaaaa
     4681 atatacgcgg agctcagcgt ttgaaattcg ttgggcgaaa gcagtttttg tgaaaagagc
     4741 tgtttgagtc ctttgagtgt gtttc
//
LOCUS       DAOQNQ010000248         4765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_7603_length_4765_cov_4.613376, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000248 DAOQNQ010000000
VERSION     DAOQNQ010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7603_length_4765_cov_4.613376"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..521)
                     /locus_tag="PK754_12660"
     CDS             complement(57..521)
                     /locus_tag="PK754_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10403.1"
                     /translation="MELAWPYELQSDLLKLGFTTSGVTPFQVEMVQKEREFVAGVIAQ
                     FTAKRVRLVLEPEKIIRPVERVAYQDEPVPEPSPAENFFDHYPTLDAAQEPFTEADVK
                     PDGNEAQISVQVRSDLFKYVRDKEAEMPLKRMIELLDCELIDITNKDTLTTS"
     gene            complement(716..1882)
                     /gene="dnaX"
                     /locus_tag="PK754_12665"
     CDS             complement(716..1882)
                     /gene="dnaX"
                     /locus_tag="PK754_12665"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNB10404.1"
                     /translation="MSYLVIARKYRPMVFDDVIGQEHITTTLKNAIESKRIAHAYIFS
                     GPRGVGKTTSARIFAKAINCENGGPTITPCNQCQSCLEITGGRSLDVLEIDGASNRGI
                     DEIRSIRDNVRYLPVRGGYKIYIIDEFHMITKDAFNALLKTLEEPPPQVVFIFATTEP
                     HKILPTILSRCQRFDFKRVAIEKIIGRLKHICRIEQIDMDEESLFEIAQMADGGMRDA
                     LTLLDQVISFCGNQVRIQRVREALGLIETGLYFELMAIIRNKSVSEGFKFASRVLDAG
                     LDVTEFLHGLMAHIRNVLLAKACGASALTTVYESYHEKFTDVSQQFSETDLIRMMNLV
                     SETIQRVKWSLKQRFVFELMLMKLIRMEDSVQLSEIIRKLENPDTETVKKKVVP"
     gene            complement(2047..2145)
                     /gene="ffs"
                     /locus_tag="PK754_12670"
     ncRNA           complement(2047..2145)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK754_12670"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(2241..2324)
                     /locus_tag="PK754_12675"
     tRNA            complement(2241..2324)
                     /locus_tag="PK754_12675"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2288..2290),aa:Tyr,seq:gta)
     gene            complement(2349..2421)
                     /locus_tag="PK754_12680"
     tRNA            complement(2349..2421)
                     /locus_tag="PK754_12680"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2386..2388),aa:Thr,seq:tgt)
     gene            2624..3901
                     /locus_tag="PK754_12685"
     CDS             2624..3901
                     /locus_tag="PK754_12685"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNB10405.1"
                     /translation="MSEISRIEILLFFLLIFIGGFFSSFETAILSLDKLSFKKILKRG
                     GTRSKLLELWTSNQNDFLTGIYVGNNAFNVGAAVLAASVSVDIARLYGYREAYALAVA
                     TFLITLFLLLFSEVLPKSIARHNPEKTVMLLATPLHIALLILKPFSGFFSWVSRVVVT
                     ATGGETHRRSLNVTEDEIREIINAGEVAGAIEHNEREMIHSIIEFGDTLVKEVMVPRV
                     DMVCVEVQTPMEEILQIMADEKLSRIPVYEENMDTVVGVLHIKNVMNFWRKNIQDMTA
                     IEFITMPYFVPETKKISELLREFQSQRIQMAIVVDEYGGTAGLVTIEDLIEEIVGEIK
                     DEYDDNVSLIKKQEDGSYLADAKIEIYLINEQLHLHLPSDEYSTLSGLILWLFKRMPK
                     KGDTISYDDVRFTIIESDRKRIHKVLIQIPSPP"
     gene            3992..>4765
                     /locus_tag="PK754_12690"
     CDS             3992..>4765
                     /locus_tag="PK754_12690"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNB10406.1"
                     /translation="MITIYAQLFGKEFVNTLTFDELVIYLKDPQCIFWIDLNSPTLDE
                     IHQIQDAFNFHPLCIEDVMSYSNSPKLDEFDEYIFLVTHEPRMHPETNEIERPEIDFF
                     LGKNYLVSVHHEPSPAIAKSIHRCETQLLFHQAALYEKGSRGRTTIKDNFMFKNSDFI
                     LHTILDHIVDDYFPLVEKWEDDIDQLEEHVLSVRAERSVLNEILKLKRQLAGFRRTVS
                     PQRDVLSRLIHLQHPAMSKASVVYFRDIFDHLIRVNELID"
BASE COUNT         1294 a         1124 c         1012 g         1335 t
ORIGIN      
        1 cgcttctttc atcaggctct gcatatcaaa tccacgcata cgatctccat aaatgattaa
       61 gatgtagtaa gggtgtcttt attggttatg tctatcaatt cgcaatccaa aagttcgatc
      121 atgcgtttga gcggcatttc cgcttcttta tcacgcacat atttgaataa atccgaacgt
      181 acctgcacac tgatctgcgc ttcgttgccg tccggtttta catcggcctc ggtaaacggt
      241 tcttgtgccg catccaacgt cggataatga tcaaagaaat tttcggccgg cgacggctca
      301 ggtaccggct catcttgata ggctacgcgc tctaccgggc gaatgatttt ttccggctcg
      361 agaaccaaac gaacacgttt cgctgtgaat tgagcgatca caccggctac aaattcacgt
      421 tctttttgta ccatttcgac ctgaaacggt gtcacgccgc tcgtggtaaa acccaatttg
      481 agtaaatcgc tttgcaattc gtaaggccag gccaactcca atacggaccc gacgcgacgc
      541 ttctcgcggt tgacttcgac gacaattttt gaccaagatt tacgaatttc gcttaatcct
      601 cgttcaccgt ttatcggtgg ttctttgggc ggttccgtga caactaccgg cgttggtatt
      661 tcggaatgtt tgacggctgg cggtttaaga ggcgttacag gatttgcggg agccgttacg
      721 gcactacttt tttttttacg gtctccgtat cggggttctc cagttttcgg atgatctccg
      781 aaagttgaac agaatcttcc atacgaataa gcttcatcag catcaattcg aatacaaagc
      841 gttgcttgag gctccatttg acgcgttgaa tggtctccga taccaaattc atcatgcgaa
      901 taaggtcggt ttcagaaaat tgctgcgata cgtctgtaaa tttttcgtga taactctcat
      961 acaccgtcgt caatgccgat gcgccacagg ccttggccag cagtacattg cgtatatgcg
     1021 ccatcaatcc gtgcagaaac tccgtcacat caagacctgc atccagtacg cgcgaggcaa
     1081 atttgaatcc ttcggaaaca cttttattgc gtatgatcgc catcagttca aaatataacc
     1141 ctgtttcgat cagcccaagc gcctcacgca cgcgttggat acgcacctga ttaccgcaaa
     1201 aggaaatcac ctgatccagc aaggtcagcg cgtcacgcat accgccgtcc gccatttggg
     1261 cgatttcaaa cagtgattct tcgtccatat caatttgttc gatacggcag atatgtttga
     1321 gtcgtccgat gattttttcg atagcgacgc gtttaaaatc gaagcgctgg caacgtgata
     1381 agatcgtcgg caggattttg tggggttcgg tcgtcgcaaa aataaatacg acctgtggtg
     1441 gcggttcttc gagtgttttc aggagcgcat taaacgcatc cttggtgatc atgtgaaatt
     1501 cgtcaatgat ataaatcttg tatccgccgc gtaccggcaa ataccgtaca ttgtcacgaa
     1561 tactgcgaat ctcatcaata ccgcgattcg aagcaccgtc aatttccaac acatccaaac
     1621 tgcgtccgcc ggtaatttcg aggcaggact ggcattgatt gcacggcgtg attgtcgggc
     1681 cgccattttc acagttgatg gctttagcaa aaatacgcgc cgacgttgtt ttgccgacac
     1741 cgcgcggacc ggaaaagata tatgcatgcg cgatgcgctt agattcaatc gcatttttca
     1801 gcgtcgtggt gatatgttcc tgaccgatca catcatcaaa aaccatcggc cgatattttc
     1861 gcgcaattac aagataggac atagatattt ggttaaaatt taatgccggc caagataaaa
     1921 gattttggga ataaaaaaaa tggaatttta tacgtaagaa gtttcttata aaacggatcg
     1981 aaagaacacg aatcagcaac taattatcat tttctatccg gatattcagc gtcaaaaaag
     2041 aataaaaaag cccaagctgt tacggcacat ccaagggtga gcttaccgtt gcttccttcc
     2101 ggacctggcg gggttcagcc tcctcaaatt gcgtaaggct cgggcgttaa taaaacttat
     2161 ttcaaaacaa catgcgttca acgtcaatat aacataacgt cgtaaacaat aaaaccccgc
     2221 ctaggtagcg aggtttttcg tgggcagtga aggatttgaa cctccgtaga gcatagctcg
     2281 acagatttac agtctgttgc gattgaccac tccgccaact gcccaatgaa cttttactgc
     2341 ataagtagag ctggtgggcg gatttgaacc gtcgacctac tgattacaag tcagttgctc
     2401 taccagctga gctacaccag caatatctct aatgtctaag aacattctca aaattcgggc
     2461 gaaaatatag atgatgccgc acactttgtc aatgaatatt ttatgtattt catgaattct
     2521 tcgccatatc taaccctgat gcttgacatt caatcctgtt ttgtttacca tgacgccaca
     2581 ctatgaaact gtatttgttc tatcccgctc ccctcttcct gatatgagtg aaatatcacg
     2641 catcgaaatt ttactctttt ttctgcttat tttcatcggc ggattcttca gcagttttga
     2701 aacggccatt ttatcgttag ataagctgtc attcaaaaaa attttgaaac gcggcggcac
     2761 gcgctccaaa ctacttgagc tctggacatc caatcaaaat gattttttaa cgggtatcta
     2821 cgtaggtaat aacgcattta atgtcggcgc cgccgtattg gccgccagtg tcagtgtgga
     2881 tattgcgcga ctgtacggct accgcgaagc ttacgcttta gccgtggcca cctttttgat
     2941 tacgcttttt ctcctgctat tcagcgaagt attgcccaaa tccatcgcgc gccataatcc
     3001 ggaaaaaacg gttatgctgt tggcgacgcc gctgcatatt gcgttactga tcctcaaacc
     3061 cttcagcgga tttttttcat gggtttctcg tgtcgtcgtc accgcaacgg gcggtgaaac
     3121 acatcgccgc agcctcaatg taaccgaaga tgaaattcgc gaaatcatca atgccggcga
     3181 agtggcgggt gcgatcgaac acaacgaacg tgaaatgatt cacagcatta tcgaattcgg
     3241 tgatacgctg gtcaaagaag tcatggtgcc tcgcgtggat atggtatgtg tggaagtcca
     3301 gacaccgatg gaagaaattc tccaaatcat ggcggatgaa aaactgtccc gcatccctgt
     3361 ctatgaagaa aatatggata ccgtcgtcgg tgttctgcat atcaaaaacg tgatgaattt
     3421 ttggcgtaag aacattcagg atatgacagc tattgaattc atcaccatgc cctattttgt
     3481 tccggaaacc aaaaaaattt cagaattatt gcgcgaattt caatcgcaac gtatccagat
     3541 ggcgatcgta gtggatgaat acggcggaac ggccggtttg gtgacgatcg aagatttgat
     3601 cgaagaaatc gtcggtgaaa tcaaagatga atacgatgat aatgtttcac tgataaaaaa
     3661 acaggaagac ggctcctatc tcgccgatgc aaaaatcgaa atttatctca ttaatgaaca
     3721 attgcattta catctgcctt cagacgaata cagcaccctc agcgggttga tcctttggct
     3781 tttcaaacgc atgcccaaaa aaggtgatac catctcctat gacgatgttc gatttacgat
     3841 catcgaatcg gaccgaaagc gcatccacaa ggtactgata caaataccgt cgcccccata
     3901 gttccatcga cgacccaaac cgggataaat catttgaata atcagttcca ctgtattaca
     3961 ttgcgtcgct tttttaaacg acttttacga tatgatcacc atttacgcgc aattgttcgg
     4021 caaagaattt gtcaatacgc ttacgtttga cgaattggtg atttatctca aagacccgca
     4081 atgtattttc tggatagatc tcaattcccc cacattagac gaaattcatc aaattcagga
     4141 tgctttcaat tttcatccgt tgtgcatcga agatgtgatg agttattcca attcgcctaa
     4201 attggacgaa tttgacgaat acattttcct cgtaacacac gaaccccgaa tgcatccgga
     4261 aaccaatgaa atcgagcgcc cggaaattga ttttttcctg ggtaaaaatt atctcgtttc
     4321 cgttcatcac gaaccaagcc cggctatcgc caaatctata caccgatgcg aaacgcaatt
     4381 gttgtttcat caggctgcac tatatgaaaa aggttcgcgt ggtcgaacga cgatcaaaga
     4441 taactttatg tttaaaaatt cagattttat tttacatacg atcttagatc acatcgtgga
     4501 tgattatttt cctttggttg aaaaatggga agacgatatt gatcagctcg aagaacatgt
     4561 tctgtccgtt cgcgccgaac gttcggtgct caacgaaatt ttaaaactca agcgacagtt
     4621 ggccggtttt cgccgcactg tatcccctca gcgggatgta ctttcacgcc ttattcacct
     4681 ccaacatccg gcgatgtcca aagcctcggt agtctatttt cgcgatattt ttgaccacct
     4741 tattcgtgtc aacgaactga ttgat
//
LOCUS       DAOQNQ010000249         4720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_7717_length_4720_cov_4.144051, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000249 DAOQNQ010000000
VERSION     DAOQNQ010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7717_length_4720_cov_4.144051"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..713
                     /locus_tag="PK754_12695"
     CDS             87..713
                     /locus_tag="PK754_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNB10407.1"
                     /translation="MPKGIITRLPGSDSVVALTLDACESLIPSYFDTTILNYLLLERI
                     PFTLFMTGNFAVRNRETLRMLAGYEWIDIQNHSYHHHLHMESLSDSVVIAEVKSAEDT
                     IFSITGRRTKLFRFPGGNYDTRTLKRVEHMGYRVVHWSFESGDPDKKITAQKMIPWVT
                     SKTRPGGILIFHINGRGYATGKALPEIVTHLRNTGYHFAKLNDYTFTE"
     gene            complement(721..2196)
                     /locus_tag="PK754_12700"
     CDS             complement(721..2196)
                     /locus_tag="PK754_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNB10408.1"
                     /translation="MAKPRTLGELKSSGYQTLSVKDEMRRNLMRKLKQGETIFPNIIG
                     YDKTVIPELIHAILSKHDIILLGLRGQAKTRILRSLTNLLDEEIPVIEEAPLNDNPFD
                     PVSRAAKDAVEKYGDATPITWWPRHERYGEKLATPDVTVADLIGDIDPIKAATQKLHY
                     ADERVMHFGVIPRSNRGIFAINELPDLQPRIQVSLFNILEEKDFQIRGFNIRIPLDIM
                     IVFSANPEDYTNRGSIITPLKDRIDSQILTHYPTQIEDGIRITRQEAWEDRNPATIAI
                     PDYFREIVENIAFQARGSEFIDQKSGVSARLTISCMENLLSTIERRMILLHEETSFPR
                     ICDLFYLIPSITGKIELVYEGEQQGMASVAKVLIGRAVKATFKQYFPDPLNKKNLPTY
                     QSILSWFSAGNKLELSNEESFASYNAKLLSIPGLRDVAAKYMKTENKDAMTLATAMEF
                     VLEGLHQNSSLSKDWIESHVSYKDIMGTMLGKINWEKEEEE"
     gene            complement(2333..2950)
                     /locus_tag="PK754_12705"
     CDS             complement(2333..2950)
                     /locus_tag="PK754_12705"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNB10409.1"
                     /translation="MSQESGNNSKNLLYAGAALGALLLIGMIILFVKVTSLEKGGSKV
                     GFIKSDALLAQYKPALAIQQKLQEETAPFQADLQKRYAELQTIGEELEKKSKVLSSAA
                     MAPQMESFQRKQSEFYQLQQSLQQQVNQKQSQLMEPIFQDISTFIQKYGKDNGYTVIL
                     GTPVEGLIVYGETGHDLTETLLNELNSKVPPSMPVPFSGKDTTKK"
     gene            complement(2969..3787)
                     /locus_tag="PK754_12710"
     CDS             complement(2969..3787)
                     /locus_tag="PK754_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10410.1"
                     /translation="MQLIYAILRYRFFWYAVLAITIVVGAGWLIKDLRRAIDGEYEAF
                     LLILGFVSVLTCVVFLIRRYWSYILDDRDVMEVFAVAATHNHVQLKHFHREHATSRGL
                     ISERGCSYSIRDGQTDIRISLTFLGKETADQKTIIGEQGLITIAVRVPQPFQMVIVNP
                     RVDIRESLDVEFYAPMVLNARRYAYQRHGIVFSNYDKETIQHTFENPFIQTELIELFE
                     KCRFKFAVFNERHAIAVKSATVENLARDTEMFPVLMNISTLIAADQHKQIPTLS"
     gene            complement(3789..4451)
                     /gene="plsY"
                     /locus_tag="PK754_12715"
     CDS             complement(3789..4451)
                     /gene="plsY"
                     /locus_tag="PK754_12715"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNB10411.1"
                     /translation="MISLLVILISAYLLGSISPSILISRWVKGVDIRTYGSGNAGMTN
                     AIRLLGGRWGAVVAIIDILKGLISSLWLTDAIYSAFDTTPIEITLVRILAGTAAVAGH
                     IWTIFFGFKGGKGVLTISGAVLGVAPLPIGICFATFTLIFALTRYVSLGSIIGAWVFP
                     IVVFIQRFVLQQPVSDYLLGFSLFVMALITFTHRENIRRLMRGEEKKFGAAKSTVPNQ
                     AV"
BASE COUNT         1269 a         1009 c         1130 g         1312 t
ORIGIN      
        1 gcaaaaatga tcaaacaaaa aattcggtca acctatcggc cgattcaaac atcgtaaaaa
       61 acgatacaat gttgctaacc aaagtaatgc ctaaagggat tattacacga ttgccgggtt
      121 cagattcggt tgtagcgctg acattggatg cctgtgagtc actgatccct tcttattttg
      181 acactacgat tttaaactat ttgttgcttg agcgtattcc gtttactctg tttatgacgg
      241 gcaattttgc cgtacgaaat cgggagacat tacgtatgtt ggcgggttat gaatggatag
      301 atattcaaaa tcattcctac catcatcacc tgcacatgga gtcgctgagt gacagtgtgg
      361 ttatagccga agtgaaatcg gcggaagata ctattttttc gatcaccggt cggcgaacaa
      421 agttgtttcg ttttccgggc ggaaattacg atacgcggac attaaaacgt gtcgaacaca
      481 tgggctatcg cgtcgtgcac tggagtttcg aaagcgggga tccggacaaa aaaattacgg
      541 cacaaaagat gattccgtgg gttacatcca aaacacggcc ggggggtatt ttgatttttc
      601 atatcaacgg ccgcggatat gctacgggca aggctttacc ggaaatcgta acgcatctgc
      661 gtaacaccgg ttatcacttt gcgaagctta atgactacac cttcaccgaa taaacgattt
      721 ttattcctct tcctcttttt cccaattgat tttaccgagc atcgtaccca tgatgtcttt
      781 gtagcttaca tgcgattcga tccagtcttt gcttaacgac gaattttgat gaaggccttc
      841 caaaacaaac tccatcgctg ttgccaaggt catcgcgtct ttgttttccg ttttcatgta
      901 tttggcggcg acatcgcgta agccgggaat ggataacaat ttggcgttgt acgatgcaaa
      961 cgattcttcg ttggaaagct ccagtttgtt gccggcggaa aaccaactaa ggatggattg
     1021 gtacgtcggg agattttttt tgttgagcgg gtcgggaaag tattgtttaa atgtcgcttt
     1081 gacggcacgc ccgatcaata ctttggcgac agaggccata ccttgttgtt cgccttcata
     1141 gacgagttcg atttttccgg tgatcgaagg gatgagataa aacaaatcgc agatacgcgg
     1201 aaaagatgtt tcttcgtgca atagtatcat gcgtcgctcg atggtgctca gaaggttttc
     1261 catgcacgaa atcgtaagcc gggcgcttac tccggatttt tgatcaatga attcactgcc
     1321 gcgcgcttga aaggcgatat tttcgacaat ctcacggaaa taatcgggta tggcgatcgt
     1381 agccggattt cgatcttccc acgcttcctg acgtgtgata cgaatgccgt cctctatttg
     1441 tgtggggtaa tgggtcagga tttgcgagtc aatacgatcc ttgagcggcg tgataatgct
     1501 gccgcgattg gtataatctt cgggatttgc cgaaaagaca atcatgatgt ccagcgggat
     1561 acgaatattg aatccgcgaa tctgaaaatc tttttcttcc aaaatattaa acaaactaac
     1621 ctgaatgcgg ggttgaagat cgggtaattc attgatggca aaaatgccgc ggttgctgcg
     1681 agggatgacg ccaaagtgca tgacgcgttc atcggcgtag tgaagttttt gggtagccgc
     1741 tttgatcggg tcgatatcgc cgatcagatc ggcgacggtt acatcgggtg tggccaattt
     1801 ttcgccgtag cgttcatggc gcggccacca tgtgatcggt gtggcatcac cgtatttttc
     1861 cacggcatct ttggccgcac gcgagaccgg atcaaagggg ttgtcgttga gcggtgcctc
     1921 ttcgatgacg gggatttctt cgtctaaaag attggtgagg ctgcgcagaa tacgtgtctt
     1981 ggcttgtccg cgtaatccta ataggataat atcgtgtttg gataaaatag catgaatgag
     2041 ttcaggtata acggttttat catagccgat gatattgggg aaaattgtct ctccctgttt
     2101 cagtttgcgc atcagattgc gccgcatttc atctttgacc gataaagttt gatagcctga
     2161 agatttgagt tcgccgagcg tccgcggttt tgccatatat atcctctatt gtaaagtggt
     2221 cttaatctaa cacgattata gatgatcttc aacgctcagg tttgagaatt gtgctgataa
     2281 aaaaagccgc ttcggtttaa cgaagcggct tatgtcaaca atgtagatat atttattttt
     2341 tcgtcgtgtc tttaccgctg aaaggtaccg gcatggacgg aggtactttg gagttgagtt
     2401 cgttgagcag cgtttctgtc aggtcgtggc cggtttcgcc gtatacgatc aatccttcga
     2461 cgggcgtgcc aaggataacg gtatatccgt tatctttacc gtatttttga ataaacgtgc
     2521 tgatgtcctg gaaaataggt tccatcagtt gggattgttt ttgattgacc tgttgctgca
     2581 gtgattgctg caattggtaa aattcggatt gtttacgttg aaaggattcc atctgcggtg
     2641 ccatagcggc cgaacttaag actttagatt ttttttcaag ttcttcgccg atggtttgca
     2701 gctccgcata acgcttttgc aagtcagctt ggaaaggcgc tgtttcttct tgcagttttt
     2761 gctgaattgc cagcgcgggt ttgtactgtg cgagcaacgc atccgatttg atgaagccga
     2821 ctttggaacc gcctttttcg agcgaagtta ctttgacgaa caaaatgatc ataccgatca
     2881 gaagcaaagc gccgagcgcc gcgccggcgt aaagcaggtt tttagaatta tttccggatt
     2941 cttgagacat aggattctcc ttgttgtttt atgataatgt aggaatttgt ttgtgttgat
     3001 cggcggcgat caaggtgctg atattcatca gcacgggaaa catctccgta tcacgcgcca
     3061 aattttcgac cgttgcggac ttgacagcga tggcgtgacg ttcattgaat actgcaaatt
     3121 tgaatcggca tttttcgaat aattcgatca attccgtctg aataaaagga ttttcaaacg
     3181 tatgttgaat cgtttcttta tcataattgg aaaaaacgat accgtggcgt tggtatgcat
     3241 accggcgcgc attgagcacc atcggtgcat aaaattctac gtccaaagat tcgcgaatat
     3301 ccacacgcgg attgacaata accatctgaa acggctgggg cacccgtaca gcgatggtga
     3361 taagcccttg ctcaccaata atcgtttttt gatccgcagt ttcttttccc aaaaaagtta
     3421 atgatatgcg aatatccgtc tggccgtccc gaatactata actgcaaccg cgttcgctga
     3481 tcaatccccg ggatgtcgcg tgctcccgat gaaaatgttt aagttgtaca tggttatgtg
     3541 tagccgctac ggcaaacact tccatcacat cgcgatcatc caaaatataa gaccaataac
     3601 gtctgatcaa aaagaccacg catgtgagaa cggaaacaaa tcctaggatc aaaagaaagg
     3661 cctcgtattc gccgtctata gcgcggcgga gatctttgat gagccaaccc gctccgacaa
     3721 cgatagtaat ggccagcacg gcataccaaa aaaaccgata acggaggatt gcgtaaatca
     3781 actgcacgtc agacggcctg attgggtaca gtggatttag cggcgccgaa ttttttttct
     3841 tcgccacgca tcaagcgccg tatattttcg cgatgagtaa aagtgatgag cgccatgaca
     3901 aagagactaa aacccaacag gtaatcggaa acaggttgtt gtaacacaaa tcgttggata
     3961 aaaacaacaa taggaaaaac ccatgctccg atgatcgaac ccagtgaaac ataacgcgtg
     4021 agtgcgaaaa tcagtgtaaa ggttgcgaaa caaattccga taggcaacgg cgccacgccc
     4081 aatactgcac ctgatatagt cagaacgcct ttaccgcctt tgaatccaaa aaatatagtc
     4141 catatatgtc ccgcaacggc tgcagtcccc gccaagattc gcaccaaggt gatctcaatc
     4201 ggtgtcgtat caaatgccga atagatggca tcagtaagcc acaacgaaga aatcaggcct
     4261 ttaagaatat caatgatggc tacgactgca ccccaacgcc cgccaagcaa tcggatggca
     4321 ttggtcatgc ccgcgttacc actgccgtag gtacgaatat ccacaccttt tacccagcga
     4381 gatattagaa tgctagggct gatcgagcct aagagatacg cgctaataag tataactaag
     4441 agtgaaatca taatattttc tttgttactg aacggcgcat gatacgtatt aatgaaagaa
     4501 aattcaatgt aatatttgaa atgtatggga catctcaaaa tacatcaaac cctcatgttg
     4561 accaaacatg agggtttgta ttacaaaaag gaggaggatt taaaatgtgt ggaggctaaa
     4621 gattagtatg acgaaatcca tcttgtgagc cagtgatatg tccacggagg cgttggctct
     4681 ttttcaggct tgagatgatt cccgggatat cattcatatc
//
LOCUS       DAOQNQ010000250         4690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_7812_length_4690_cov_5.495793, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000250 DAOQNQ010000000
VERSION     DAOQNQ010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7812_length_4690_cov_5.495793"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PK754_12720"
     CDS             <1..381
                     /locus_tag="PK754_12720"
                     /inference="COORDINATES: protein motif:HMM:NF012410.2"
                     /note="molecular chaperone; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNB10412.1"
                     /translation="AEKKYGKLIDLLKDQLNEEIKDVRVSARLKDSACCLVADEDGMD
                     QNMERIMKALGQNVPSGKRTLEINPDHPLIGKMQMMFDIDKDQPLLKDYTALLYDQAL
                     VLEGSKPKDPVLFAKRIAEIMVKA"
     gene            609..1973
                     /locus_tag="PK754_12725"
     CDS             609..1973
                     /locus_tag="PK754_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNB10413.1"
                     /translation="MISGKDLIAMGYKPGKWFKDAIEHINENHLTDEKLENYLQQISP
                     KEVLPLDKAIPYFSNIRAENEVELENIKSVDTTMMELMKTPTMIKGAIMPDACPTGEK
                     GQIPVGGVAVAQNAIHPAMHSADICCSVMMTGFGKIAPKSVLDAAHAITHFGPGGRDE
                     FSNLPSELVEQICSNPFLNNERSKTLAATHLGTQGDGNHFLFVGVSAKSGETIMVTHH
                     GSRGFGAQLYAQGMKMAEFFRKEISPKTRACNAWIPFDTGEGNNYWEALQIVRVWTKL
                     NHRVIHDAVLHSLHADITERFWNEHNFVFREKDLFYHAKGATPLDDKFVPDSTDGLRL
                     VPLNMSQPVLVVKGATTKNNLGFAPHGAGRNISRGQHKRLYAHKTKEQLFAEETRGLD
                     IRFFSRHIDVSELPSAYKNADAVKAQMKEFGLGEILDEIIPYGCIMAGDWEKNAPWRK
                     KKNK"
     gene            2444..>4690
                     /locus_tag="PK754_12730"
     CDS             2444..>4690
                     /locus_tag="PK754_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10414.1"
                     /translation="MKKVVFYWLVTVLCLTSFAAAQYDSSLFSGMKARSIGPAGMSGR
                     IASIDAVKSDPNIIYVGAATGGVWKSINGGITWNSIFDDQSTGSIGSVAIFQKNPNIV
                     WVGTGESNVRNSVGVGRGIFKSIDAGKTWQFFGLEKAEKIARVILHPENADIAWVAAM
                     GTTWGENKERGVYKTTDGGKTWKQVLYVDEKTGAAELVMDPSNPNKLLCGMWEHRRYP
                     WYFKSGGTSSGLYISTDGGETWKKQTTKEGLPEGELGRIALSFSRNKTSTVYALVEAS
                     ESVLLRSDDGGYKWRTVNKEKNVHPRPFYFSSIEVNPVNENIVYRIHVTFDVSEDGGK
                     SFSLLTPYQNIHPDFHALWMHPNGEMMIAGNDGGLAFTYDRGKTWRFVQNLPVGQFYH
                     ISYDMEYPYNIYGGLQDNGSWRGPSTTFKRDAIYNDVWDMVAFGDGFDTEPDPENADF
                     GYAMSQGGALVYFNHKTGLQKFIRPTESAVKHRYNWNAGFALDPFDAKTIYYGSQFLH
                     KSTDKGDTWEIISPDLTTNDTTKINLGKETGGLTLDVSGAENHCTILTIAPSSVKRGV
                     IWVGTDDGNVQVTQDGGKTWTLVSKNKSIVAPAGTWVPHIEASKFDAGTAYIVFDDHR
                     RSNWSTYVYGTRDFGKTWTNLATKEVDGFVHCFEEDPVNKDLLWLGTEYGLYVSFNSG
                     KNWMKWRAGVPTVPVYDLATHPREHDLIIGTHGRGIYLLDDITPLRKFAENSKKNAHL
                     YEVNPGYQY"
BASE COUNT         1331 a         1066 c         1089 g         1204 t
ORIGIN      
        1 gccgaaaaga aatacggcaa actgattgac ctgctcaaag atcagttgaa cgaagagatc
       61 aaagatgtac gggtttcggc acgactcaaa gattcggctt gctgccttgt tgccgacgaa
      121 gacggtatgg accaaaacat ggaacgaatc atgaaagccc tgggtcaaaa tgttccgtcc
      181 ggcaaacgta cgctcgaaat caatccggac catcctctga tcggtaaaat gcagatgatg
      241 tttgatatcg ataaagatca accgttgctc aaagactata cggcgctgtt gtatgatcag
      301 gcgctcgtac tggaaggcag caaacccaaa gatccggtgc tttttgccaa acgtatcgct
      361 gaaataatgg taaaagcata aaaaggattt gcaaaacaaa aatggatcgg ttataattct
      421 gcgctatgat aaagaacaac aacatacgtt attaccgttc aaacggactt atccttcgtc
      481 gtcatacacg tatggatcaa gtcaatttga tatattggag cgcatgagcc gattttaatt
      541 ttttgaatat tttttctaac cccggctccg tgaacagccg gggtttttta ttttactaaa
      601 ggagtattat gatctcgggg aaagatctca tcgctatggg ttacaaaccc ggcaaatggt
      661 tcaaagatgc cattgaacac atcaatgaaa atcatctaac ggatgaaaag ttggaaaatt
      721 atttacagca gataagtcca aaggaagtgt tgccgctcga taaagctatt ccatacttca
      781 gcaatattcg cgctgaaaat gaggtagagc ttgaaaacat caaaagcgtt gacaccacaa
      841 tgatggagct gatgaaaaca ccgacgatga tcaaaggcgc tatcatgccc gatgcatgtc
      901 cgacgggtga aaaaggccaa ataccggtcg gcggcgtagc cgtcgcccaa aacgctattc
      961 accccgctat gcacagtgcc gatatttgtt gctcggtaat gatgaccggg tttggcaaaa
     1021 tagctcctaa atcggtgtta gatgcggcac atgcgataac ccatttcggt ccgggtggtc
     1081 gtgacgaatt ttcaaatctc ccgagcgaat tggtcgaaca aatctgttcc aatccgtttc
     1141 tgaacaatga acggagcaaa acattggctg caacgcattt gggtacgcaa ggtgacggca
     1201 accatttttt gtttgtcggg gtatcggcca aaagcggcga aacgatcatg gtcacccacc
     1261 atggtagccg agggttcggc gcacaactgt atgcacaggg aatgaaaatg gcggagtttt
     1321 tcagaaagga gatctcaccc aaaacacgcg cctgcaatgc atggattcct tttgatacgg
     1381 gcgaaggcaa taactattgg gaagccttac aaatcgtgcg cgtatggacc aaacttaacc
     1441 accgtgttat tcacgatgct gttttgcatt cgctgcatgc agatattacg gagcgttttt
     1501 ggaacgaaca taatttcgtc ttccgtgaaa aagacttgtt ttatcatgcg aaaggcgcta
     1561 cgccacttga tgataagttt gttcccgatt ccacggatgg tttgcgcttg gtaccgctca
     1621 atatgagcca acccgtactc gtcgtcaaag gcgcgaccac aaaaaataat ctgggttttg
     1681 cgccgcatgg cgccggccgt aatatcagcc ggggccaaca caaacggttg tatgcgcata
     1741 aaactaagga gcagttgttt gctgaagaaa cacgcggttt ggatatccgc tttttcagtc
     1801 gtcacattga tgtatccgaa ttgcccagcg catacaaaaa tgcagatgct gtcaaagcgc
     1861 agatgaaaga attcggtcta ggcgaaatcc tggacgagat tattccctac ggctgtatca
     1921 tggccggcga ttgggaaaaa aatgcgccgt ggcgaaagaa gaaaaacaaa tagcattaga
     1981 tggacaacac cggaaaccgc taaaggttat tccggtgttt gttttatggg tatcatcgta
     2041 tcatgtttaa aagagatgct tcgtacccgt atgcattgtt aggttcattg gcagacacga
     2101 caattatctt attctgaaat cttgtttttt ctctcattat tgaaacaatt aagtagcaac
     2161 ttacgtaaag gtcttttact tttccgttac gattccttag ttgttttttt cttatctatt
     2221 taagatatat aaccttacga cctgtttatt tagtggctgt gatttttgat tatttagatt
     2281 aaaaaatctg attttattga atttgagata tcatattatt tgaaaatact tggccgtata
     2341 tgtcttaaat atggcgagcg cgtaatcctg ttttgtgata tttttaaaaa actatagaaa
     2401 tgtcatcccg tcccatacgt tacttttaat ggagttttct gttatgaaaa aagtcgtgtt
     2461 ttattggctt gttaccgttt tgtgcctgac ttcgtttgct gccgcacaat acgacagttc
     2521 attattcagc ggcatgaaag cccggagtat cggtccggcc ggcatgagcg gacgtatcgc
     2581 ttcgattgac gccgtcaaaa gcgatcctaa catcatctat gtcggagccg ctaccggcgg
     2641 tgtctggaaa tccattaacg gcgggattac gtggaactct attttcgacg atcaatccac
     2701 cggctccatt ggttcggttg cgattttcca aaaaaacccc aatatcgttt gggtcggtac
     2761 cggcgaatcc aatgtccgaa actctgtcgg tgtcggtcgc ggtattttca aatccataga
     2821 tgccggcaaa acatggcaat tcttcggtct tgaaaaagcc gaaaaaattg cacgcgttat
     2881 tctacatcct gaaaacgccg atatcgcgtg ggtagccgcc atgggaacca cctggggcga
     2941 aaacaaggaa cgcggcgttt ataaaacaac cgacggcggt aaaacatgga aacaagtact
     3001 ctatgtggac gaaaaaaccg gcgccgcgga actggtgatg gatccctcca atcccaataa
     3061 acttttgtgc ggtatgtggg aacatcgccg ctacccttgg tatttcaaat cgggcggcac
     3121 aagcagcggt ttgtatatat ctacggatgg cggtgaaaca tggaaaaaac aaaccaccaa
     3181 agagggtttg cctgagggcg aactgggtcg catcgcattg tctttttcgc gcaacaaaac
     3241 ctccacagtg tacgctctgg tcgaagcttc cgaaagtgtg ttacttcgct ccgatgatgg
     3301 cggctacaaa tggcgaaccg tcaacaaaga aaaaaatgtt catcctcgcc cgttttattt
     3361 cagttccata gaagtcaatc ccgtcaacga aaatatcgtt tacagaattc acgttacatt
     3421 tgatgtgagc gaagacggcg gcaaaagttt ttctcttctg acaccgtacc aaaacattca
     3481 ccccgatttt catgccttgt ggatgcatcc caatggcgaa atgatgatcg ccggcaatga
     3541 cggcggtttg gccttcacct acgaccgcgg taaaacatgg cgctttgttc aaaacctgcc
     3601 cgtcggtcag ttttatcata tcagctatga catggaatat ccctataata tctacggcgg
     3661 attgcaagac aacggctcct ggcgcggtcc gagcaccaca ttcaaacggg atgccattta
     3721 taacgacgtc tgggatatgg ttgcgttcgg cgatggattt gatacggaac ccgatcccga
     3781 aaatgccgat tttggatacg cgatgtcgca aggcggcgct ttggtgtatt tcaatcacaa
     3841 aaccggttta caaaaattca ttcgcccgac ggaatctgcc gtcaaacacc ggtataactg
     3901 gaatgccgga tttgcactgg atccttttga tgcgaaaacc atttattatg gaagccagtt
     3961 tttgcacaag agtacggata aaggcgatac ttgggaaatc ataagccctg atctcactac
     4021 caacgacacg acgaaaatca atcttggcaa agaaaccggc ggattgacgc tcgatgtatc
     4081 cggcgctgaa aatcattgca ccattctcac catcgcgcca agctccgtaa agcgcggtgt
     4141 gatctgggtc ggaaccgacg acggtaatgt ccaagtcaca caagacggcg gcaaaacatg
     4201 gacactggtt tcaaaaaaca aatccatcgt agcgcccgca ggaacatggg tgcctcatat
     4261 tgaagcatcc aaatttgatg ccggcaccgc gtacatcgta tttgacgatc atcgccgctc
     4321 caattggtca acctacgtct atggtacacg cgatttcgga aaaacatgga ctaatcttgc
     4381 gaccaaagaa gttgacggat tcgttcactg cttcgaagaa gatccggtaa acaaagatct
     4441 gctgtggctt ggcaccgagt acggacttta cgtttctttt aacagcggca aaaattggat
     4501 gaagtggcgc gccggtgtac ccaccgtgcc ggtctatgat cttgcaactc atccgcgcga
     4561 acatgatctc atcatcggta cgcacggacg cggcatctat cttctcgatg atattacacc
     4621 gctacgaaag tttgctgaaa actccaaaaa gaacgcgcac ctgtacgaag tcaatcccgg
     4681 ctatcagtat
//
LOCUS       DAOQNQ010000251         4686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_7824_length_4686_cov_5.592529, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000251 DAOQNQ010000000
VERSION     DAOQNQ010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7824_length_4686_cov_5.592529"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PK754_12735"
     CDS             complement(<1..192)
                     /locus_tag="PK754_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10415.1"
                     /translation="MSKKHVLGKWVQLPDTLTFCGEVIPLNDPDVRERAEDIFYTRLG
                     DDDRFFLLLRRTSRYFPFYE"
     gene            complement(792..1871)
                     /gene="mltG"
                     /locus_tag="PK754_12740"
     CDS             complement(792..1871)
                     /gene="mltG"
                     /locus_tag="PK754_12740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNB10416.1"
                     /translation="MVIDDPRSEARKRLLQYIFIGISILVVATLLVVVSFYKLVLYNA
                     PSINVEQLPLDKKLLITIPKGASLNGISNVLQESGVIENATLFRLAARYLGAERSLKA
                     GQYLLPAHGSNHQIIRILQNAVPQSVRVTIPEGRGLRSIIQLLSAKLPLDSAKFVNAV
                     NDSLLCRELGFPDKSLFGYLMPNTYFIDPGTSESDIVRLMIAEYTNFFGDDLKKRTAE
                     LQLTPRQIMTIASIIEGETADDDERYLVSSVYHNRVRRGMMLQADPTIQFIIKDGPRR
                     LYNKDLEIDSPYNTYKYAGLPPGPVNNPGKASILAALYPANSEYLYMVADGKGKHIFS
                     KTMEEHLRARKQLDTIRKQLEKKKK"
     gene            complement(2119..3174)
                     /gene="amrS"
                     /locus_tag="PK754_12745"
     CDS             complement(2119..3174)
                     /gene="amrS"
                     /locus_tag="PK754_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNB10417.1"
                     /translation="MRVPSSLRDNLRSLVQEGSLYKQTDDGRVQCYACGHRCIIGEGR
                     DGICKVRFNRNNKLWVPHGYTAGIQLDPVEKKPFFHVFPGSTAMSFGMLGCDLHCAYC
                     QNWITSQALQDPYAGVPPRLITAAELAQHASDHGARLLTSTYNEPLITAEWAVRVFEE
                     GQRLGLYGAFVSNGNATPEVLTFLRPYVSMYKVDLKSFNDKNYRKLGTTLKNVTDSIE
                     KIHAMGFWSEIVTLIVPGFNDSDVELQAMASFIKNISCDIPWHVTAFHPDYKMTDPDE
                     TDADTLARAAHIGKNEGLHFVYTGNRPGELRDMENTYCPGCKALLVKRHGYRIQQNRI
                     IHGKCPDCDRKIPGYWF"
     gene            complement(3194..3931)
                     /locus_tag="PK754_12750"
     CDS             complement(3194..3931)
                     /locus_tag="PK754_12750"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNB10418.1"
                     /translation="MIRITYNTPFRGTIPTSSFTYYRWETRLATDEKIRFIAIEGVLK
                     PENTALARNLSQMANARPILEAEFLSPLVNIHSTDPKDMAFKKHMLHLMDRYKMLKQI
                     HQTEIFFEQVICDYLFYADRVYANLKLDNEDMHLYDTMMNVMERELPIPDLVIYLQSN
                     IDTVMEKIHTRHALGNASKERQRMDETYIAALNEEFNQFFLYYRWSPVLIVNANQLDP
                     ENTHHLEDLYQRLDTRLSGVVYYNPPA"
     gene            complement(3840..4382)
                     /gene="folK"
                     /locus_tag="PK754_12755"
     CDS             complement(3840..4382)
                     /gene="folK"
                     /locus_tag="PK754_12755"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNB10419.1"
                     /translation="MKNKVLLSLGSNIGDRMQHLREAVDLISRFAGIKKMASIYETEP
                     VSPIMQDNYYNTAMIIDTDLDAQSLMKKLLDVEKSLGRERTIPGNARTIDLDIILFDD
                     LIMNTPDTVIPHPRFAERNFVLVPSVEIAGNWLCPIRHLTIQEILNLSRDTHHVQYAV
                     SRDNPDVIFHLLPMGDAVGH"
     gene            complement(4379..>4686)
                     /gene="folB"
                     /locus_tag="PK754_12760"
     CDS             complement(4379..>4686)
                     /gene="folB"
                     /locus_tag="PK754_12760"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNB10420.1"
                     /translation="ERRLGQRFEIDVELGLDFQPAALADDLHLSVDYGTVYQRIHTVV
                     TEKKYYLIEAVAENIAQRLLNDFPIQVVTVCVRKPSVPIHGSLDHVEVEITRERKTA"
BASE COUNT         1283 a          970 c         1093 g         1340 t
ORIGIN      
        1 ttcataaaac gggaaataac gagaggtgcg tcgtaataaa agaaagaagc gatcatcgtc
       61 acccagccgt gtataaaaaa tatcttcagc acgttcgcgt acgtcgggat cattgagagg
      121 gatgacctct ccgcaaaaag tcagcgtatc gggaagttgt acccatttac ccaaaacatg
      181 ttttttactc attgcagcgg agctcacggt ttgaccgtgc agcgatgagt tgcttaacag
      241 caataggatt gtagttatga tagcggtagg tattaaaata tttttcatag caaatcataa
      301 ttaaaaaaaa gaaaagaaaa aagcaggcat caaggatacc tgctttttca aagactgtgg
      361 aggtcgtgta accctccgca aacgacaact tacggggtgt aatccagtcg gaacccggta
      421 agtaagcgat tagaagagat tacttcttct tagcagcttt tttggtcgtt ttcttagcgg
      481 tcttcttcgc ggctttttta gcagcgggtt tcttcgcacc agctttttta gtaggcatgg
      541 tttgaactcc tttcacctgt tagtacttct gttaattaaa tgagagctcc ttcaaactct
      601 cacaaaatta ttttaaccta ttttgaaaat aaagtcaata aaattttata cttcggagaa
      661 taaaaatggt ttttgatttt atgttttttt aaacggttga tgtgtttttt tatgcgattg
      721 actgtaaaac attgtaggca gcggttctac gggttatgcg agattttgtg gtaacgtgat
      781 ggaggatggc gttatttttt ctttttttct aattgtttac gaatcgtatc caattgtttg
      841 cgtgcacgca agtgttcttc cattgtttta gaaaaaatgt gttttccttt tccatctgcc
      901 accatataaa gatattccga attggccgga tacagcgccg caagaatgga tgctttaccg
      961 ggattattta ccggacccgg aggcaggcca gcatatttat atgtgttgta aggactgtct
     1021 atttccaaat ctttgttgta caggcggcgc ggaccatcct tgatgatgaa ctgtatggtc
     1081 ggatcggctt gcagcatcat cccacggcgt acacgattat gataaacgga tgaaactaaa
     1141 tatcgctcgt catcgtccgc agtttcgccc tcaatgatgg aggcgatggt catgatctga
     1201 cgcggagtca attgcaattc agcggtccgc ttttttaagt catcgccgaa aaaattagta
     1261 tactccgcga tcatcaatcg aacgatgtca ctttcgctgg ttccgggatc aatgaaataa
     1321 gtgttgggca tcagataacc aaagagcgat ttatccggga atcctaattc acgacagagt
     1381 aaagagtcat tgacggcatt gacaaatttg gcagagtcga gcggaagttt ggccgataaa
     1441 agctgtatga tggagcgtaa gccgcggcct tccggaatcg tcacacgaac actttggggt
     1501 acggcatttt gtaatatgcg gatgatctga tgattggaac cgtgtgccgg caaaagatat
     1561 tgccctgctt tcagacttcg ttctgcgccg agatagcgag cggccaaacg aaagagcgta
     1621 gcgttttcga taacaccgct ttcctgtaag acgttcgaaa tgccattcaa cgatgcgcct
     1681 ttgggaatag taatgagtaa ctttttatct aacggtaatt gttcgacatt gatcgaaggc
     1741 gcgttataca aaacaagttt gtaaaaacta acaacgacaa gcaatgtagc aacgacgaga
     1801 atactgatgc cgataaaaat gtattgcaat aagcgcttac gtgcttcgga gcgcggatca
     1861 tcaataacca atgaattttc cttcttagtg aaaccgaaac cgcgcgaagt atataaaaaa
     1921 gaaagcttgg aatcaaatat tttggattaa actaaatttc tgtaggagat tggatcagcg
     1981 tgttgaataa gaagtattag ccattacgtt attaagaaaa gatgttaaaa aataatcaat
     2041 tgaagattaa atacatacac acttatacca gtgttattta tttcaattga aaactaatga
     2101 ttactataag ggatcaattt agaaccaata gccgggaatt ttgcgatcgc aatccgggca
     2161 ttttccgtgg ataatcctat tttgctgaat gcgataaccg tggcgtttta ctaacaatgc
     2221 tttgcacccc gggcagtacg tgttttccat atcacgcagt tcacccggac ggtttccggt
     2281 atacacaaaa tgcaaacctt cgtttttacc gatatgtgct gcgcgtgcta atgtatcggc
     2341 atcggtttcg tccggatcgg tcatcttgta gtcaggatga aacgccgtga catgccaggg
     2401 tatatcgcat gaaatgtttt ttataaatga tgccatggcc tgtagttcga catccgaatc
     2461 attaaagccc ggaacgatca gggttacgat ttccgaccaa aaacccatgg catgaatttt
     2521 ttctatgcta tcggttacat tttttaatgt cgttccgagc ttccgataat ttttgtcgtt
     2581 aaaacttttg agatcgactt tgtacataga aacatacggg cgcaaaaaag ttaaaacttc
     2641 cggtgtcgca ttgccattgg atacaaaggc gccgtataaa cccagacgct gtccttcttc
     2701 aaagacgcgc accgcccatt cggcggtgat gagcggttca ttataggtac ttgtgagaag
     2761 gcgtgcgcca tgatccgatg catgttgagc caattcggcg gcggtgataa gacggggcgg
     2821 aacaccggcg tagggatctt gcaaggcttg ggatgtgatc cagttttggc aatacgcgca
     2881 gtgcagatcg catcctagca ttccaaaact catcgctgtg ctgccgggaa acacgtgaaa
     2941 gaatggtttt ttttcgacgg gatcaagttg tataccggcc gtatatccgt ggggaaccca
     3001 taatttatta ttgcggttga agcgaacttt gcaaatacca tcgcgccctt cgccgatgat
     3061 gcagcgatga ccgcacgcgt aacattgcac acgaccgtcg tcggtttgtt tatacagtga
     3121 tccttcttgg acaagtgatc ggaggttatc gcgcaatgat gatgggacac gcatgatccg
     3181 tcctttcggg gattcaggcc ggcggattat agtaaactac gccggataag cgcgtatcta
     3241 aacgttgata aagatcttcg aggtgatggg tgttttccgg gtctaattga ttggcattaa
     3301 caataagcac cggtgaccaa cggtaataaa gaaaaaattg attgaactct tcgttgagcg
     3361 ctgcgatgta ggtttcgtcc atgcgctgcc gttctttgct tgcattaccg agcgcgtgcc
     3421 gggtgtgaat tttttccatc acggtgtcga tattactctg aagatagatg accagatccg
     3481 gtatgggtaa ttcgcgttcc atcacattca tcatcgtatc gtacagatgc atatcttcgt
     3541 tatcaagttt caggttcgca tatacgcgat cggcgtaaaa aagataatcg caaatgacct
     3601 gttcaaaaaa aatttcagtc tgatgaatct gtttgagcat tttataccga tccatgaggt
     3661 gcagcatatg ttttttgaat gccatatctt tgggatcggt gctgtgaata ttaaccaacg
     3721 gagaaagaaa ttcggcttcc aaaatgggtc gggcattggc catctgggaa agattgcgtg
     3781 cgagcgcggt gttttccggc ttaaggacgc cttctatagc gataaatcgt attttttcgt
     3841 cagtggccaa ccgcgtctcc catcggtaat aagtgaaaga tgacgtcggg attgtcccgc
     3901 gaaacggcgt attgtacgtg atgcgtatca cgggatagat ttagaatttc ttgtatcgtg
     3961 aggtgccgaa tcgggcaaag ccaatttccg gcaatttcga ccgacggtac gagaacaaaa
     4021 tttcgctctg caaaacgtgg atgggggatg accgtatccg gcgtattcat aatgagatca
     4081 tcaaacaaaa taatatccaa atcaatcgtg cgtgcgttcc cgggaatcgt tctttcacga
     4141 cccaatgatt tttctacgtc taatagtttt ttcatcaggc tctgagcatc taagtccgta
     4201 tcaatgatca tagccgtatt gtaataatta tcctgcatga tcgggctaac cggctcggtt
     4261 tcataaatgg atgccatctt tttaataccg gcaaatcgac tgattagatc aacagcttcg
     4321 cgaagatgct gcatccgatc gcctatgttg gagcctaagc taagcaggac tttattcttc
     4381 atgcagtttt tctttcacgg gtgatttcaa cctccacatg atccaaggaa ccgtgaatgg
     4441 gaacactcgg tttacggaca cagaccgtga cgacctgtat tggaaaatca ttaagtagtc
     4501 gctgcgcgat attttcagcg accgcctcaa tcaggtaata tttcttttcc gtgacgacag
     4561 tgtgaatgcg ctgatacacg gtgccgtagt ccacgctcaa atgcagatca tcggcgagcg
     4621 ccgccggttg gaaatcgagc ccgagttcaa catcgatttc aaagcgctga cctaagcgcc
     4681 gctctt
//
LOCUS       DAOQNQ010000252         4685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_7828_length_4685_cov_4.765011, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000252 DAOQNQ010000000
VERSION     DAOQNQ010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7828_length_4685_cov_4.765011"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="PK754_12765"
     CDS             <1..937
                     /locus_tag="PK754_12765"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNB10421.1"
                     /translation="SSLRIDAYSSADATGLWQFIAGTAKIWGLRVDKYVDERFHVEKS
                     TRAACALLKNLRMTFGSWSLAAAAYNTGEANIEKAVDGQHDDNYYNLFLNKETRQYVI
                     HIAVLKEILEHPEKYGYAIPDSTYFKPFDDRTETITLKGPVKDLGLWAKSIGTNYKTI
                     KMLNFWIMRYDLPEGIWELRVPEGLSRDSSLMQFWQSKNDSIIYGENNRVTIFHTVQP
                     GESQIRIAAMYSVSIQELKDWNGIKNDAVVVNQKLKLKIPAHTKIQYPVRSGDNLLKI
                     AEQFKVKAERIKEWNKRELNGLQAGEVLTIYIGKN"
     gene            complement(938..1822)
                     /locus_tag="PK754_12770"
     CDS             complement(938..1822)
                     /locus_tag="PK754_12770"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNB10422.1"
                     /translation="MRELMVSRNRLTLFLAALVSFLFISMSILGFLFSNYSGDERLAE
                     LNAENRILKDQLSDIGGRMKVIDEQLKLIRTKDSELRVIADLPDINMLLKDAGIGGSD
                     LEIDYNPDLLSDDADALVRKNLENLEKLESNIKLELQSYAELHENISANAAKLVYLPS
                     INPIKRGRITDRFGVRKSFRSFIPHTGLDIAARWGTPVYVTADGVVEGVTWRGGYGKS
                     IIVDHGNGIKTLYGHLSSYKVKNGERVKRNQKIAEVGSTGLSTGPHLHYEVRINNVPQ
                     NPELFIFFDMVDYLEMQW"
     gene            2077..3996
                     /gene="speA"
                     /locus_tag="PK754_12775"
     CDS             2077..3996
                     /gene="speA"
                     /locus_tag="PK754_12775"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HNB10423.1"
                     /translation="MTWTTDDANKIYNVEEWGAGYFHINAKGHISVTPTGDLGKSIDL
                     KTVVDDIKARGIALPVLVRFQDILRSRVRELNEHFRRAISEYNYQGKYHGVFPIKVNQ
                     LREAVEEILDAGIPYGYGIECGSKPELYVALALVEDKETLIIANGYKDAQFIRMALLG
                     KQLRKNVIIVVEKLSELRQILQISKEIGVEPAIGIRAKLSTKGTGKWEASSGDQSKFG
                     LSMPEIVEAIEMLKENNLTSAFKLLHFHMGSQIPDIRTIKAAAKEASRIYASAYKMGM
                     PIQFIDVGGGLGVDYDGSRTTFRSSMNYSLQEYANDIVYTILEVCNSEEIPHPNIVSE
                     SGRAIVAHHSVLVLEIFGNIEKKPSAFKMDFSPEDPDIIQEMLDLRHQLHKKNTNYLE
                     VYHDAAQRREEAHSLFSLGYLTLIEKAKIENLYWEIINIIREYSRSLEVIPEELAELE
                     PKLADQYLCNFSIFQSLLDHWAIDHLFPIVPIHRLNEEPTKRAILVDITCDSDGKICK
                     FIDMKDVKDKLELHPLNDDPYYIGIFLLGAYQDIMGDMHNLFGRVNEVHVFMDDEEPD
                     NYYVEEIIPGSNVGQVLSLVQYNENEIIKLIKKTVDQRIKEGQLKPSEGVKLLDTYEA
                     GLKDYTYLQLNGRSR"
     gene            4017..>4685
                     /locus_tag="PK754_12780"
     CDS             4017..>4685
                     /locus_tag="PK754_12780"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10424.1"
                     /translation="MITRTRILYLTGPLLFLLSFPPLPTGFLAYIALLPFFELLRQNQ
                     YRSGFRTGYGLGLLTMGVFIHWLNANTGATLPQATGMYFGTIMYLALYWGCFGAIQGF
                     IQRKLGERGLWLAPFLWASLDYVQSLGELGFTWHNLATTQSYYLPLIQFIDITGIYGL
                     SFWIALVNVIIFKLINAYREQQKNQAVSYAVGLGMIFLLPLVYGFLSSWRETSEKKLR
                     VAIIQ"
BASE COUNT         1348 a         1056 c         1009 g         1272 t
ORIGIN      
        1 aagctcgttg cgcattgatg catactcttc agccgatgcc acagggttat ggcaatttat
       61 tgcaggaacg gccaaaattt ggggattgcg cgtagataaa tatgtcgacg aacgatttca
      121 tgtcgaaaaa tcaactcgtg ctgcatgcgc tcttttgaaa aatctgcgca tgactttcgg
      181 cagttggagt ttggccgccg cagcatacaa tacaggtgaa gcaaatatcg aaaaagccgt
      241 cgatggccag catgatgata actattacaa tcttttttta aacaaggaaa cacggcagta
      301 cgtgattcac atcgccgtac tcaaggaaat tttagaacat cctgagaaat atggttacgc
      361 tattccggac tccacctact tcaaaccatt cgatgatcgt acggaaacaa taacactaaa
      421 aggtccagtc aaagatttgg gtttgtgggc aaaatcaatc ggtaccaatt acaaaacgat
      481 caaaatgctt aacttctgga ttatgcgcta tgatttgcct gaaggcattt gggagctacg
      541 cgttcctgaa ggcctctcac gtgattcttc gctcatgcag ttttggcaat caaaaaatga
      601 ttcgatcatt tacggcgaaa acaatcgggt gacgattttt cacacagttc aaccgggtga
      661 atcgcagata cgtattgccg caatgtatag cgtaagtatt caggaactga aagattggaa
      721 cgggattaag aatgatgctg ttgtggtgaa tcaaaaattg aaattgaaaa tccctgctca
      781 cactaagata caatacccgg ttcgcagcgg tgataatctt ttgaaaatcg ccgaacaatt
      841 taaagtcaaa gcggaacgca tcaaagaatg gaataaacga gaattgaatg gtttgcaggc
      901 aggcgaagta cttacgatct atatcggaaa gaattaatta ccattgcatt tccaaataat
      961 ccaccatatc aaagaaaata aaaagctctg ggttttgagg tacattattg atgcgcactt
     1021 cgtagtgcaa atgcggcccg gtggataaac cagtgctccc cacttcagca attttttgat
     1081 tacgctttac ccgttccccg ttttttactt tatacgatga caaatggccg tatagtgttt
     1141 ttattccgtt accgtgatct acgatgatag atttaccgta accaccgcgc caagtcaccc
     1201 cttcgacaac gccatcggcc gtaacataaa ccggcgttcc ccaacgtgcg gcaatatcca
     1261 aaccggtgtg cggtataaac gaacgaaatg atttacgaac gccgaaacga tctgtgatgc
     1321 gcccgcgttt gatcggattg attgacggaa gataaaccaa ctttgctgca ttggcgctga
     1381 tgttttcatg cagttccgca taactctgca attcgagttt aatattactt tccagcttct
     1441 ctaagttctc aagattttta cgcaccaacg catccgcatc gtccgataac aaatccgggt
     1501 tgtaatcaat ttccaaatca ctgccaccga tgccggcatc tttaagcagc atattgatat
     1561 ccggtagatc ggcgatgact cgcagttcac tatccttagt acgaatcagc ttaagctgct
     1621 cgtcaatcac tttcatacgg ccgccgatat cggaaagttg atctttgaga attcgatttt
     1681 ctgcgttgag ttcggccagg cgttcatcac cggaataatt ggaaaacaaa aaacctagaa
     1741 tactcattga aataaataaa aaactaacca aagccgccaa aaacaaagta aggcgattcc
     1801 gggaaaccat aagttcgcgc atacccgatt gattctcggg gacgtataac aagcggaacc
     1861 gtttgtcagt aaaccatttc atgggcataa aagtacataa gcatgctctt aaaggcaaga
     1921 tgttttgagg ataccgcctg cactttttcc aaaaaaagct gcggtgcaaa gacatttact
     1981 attgcatatc tatgcggttc ggtataatat tgcgcaccat taattttaac tttacaacgc
     2041 agactgaaat acctgcattt tgaggagacg ccgaagttga cctggacaac cgatgatgcc
     2101 aataaaatct acaatgtcga agaatggggt gcgggttatt ttcacatcaa cgccaaaggc
     2161 catattagcg taacgcccac cggcgatctt ggcaaatcca ttgaccttaa aacggttgta
     2221 gacgatatca aagcacgcgg catagccctg cccgttcttg ttcggtttca ggatattctt
     2281 cgaagccgcg ttcgtgagct caatgaacat tttcgccgtg ccatcagtga atacaattat
     2341 caaggcaaat accacggcgt tttcccgatc aaagtcaatc aactgcgtga ggccgtcgaa
     2401 gaaatactgg atgccggtat tccctacggt tacggtattg agtgtggcag caaaccggaa
     2461 ctgtatgtcg cgctagctct tgtcgaagat aaagaaacgc tcatcatagc caacggctat
     2521 aaagatgcgc aattcattcg tatggcgctc ctgggtaaac aactccgaaa gaacgtcatc
     2581 atcgttgtcg aaaagttatc cgaactgcga cagattttgc aaatatccaa agaaatcggt
     2641 gtcgaaccgg caatcggtat tcgcgctaaa ctcagtacca aaggcaccgg caaatgggaa
     2701 gcctccagcg gtgatcagtc taagtttggc ctttctatgc cggagatcgt cgaagcgatc
     2761 gaaatgctca aagaaaataa cctgacgagc gcttttaagt tactgcactt tcacatgggt
     2821 tcccaaatac ccgatatccg cacgatcaaa gcagcggcta aggaagcttc gcgcatctat
     2881 gcgagcgctt ataaaatggg catgcctatt caatttattg atgtcggtgg cggtttgggc
     2941 gttgactatg acggatctcg gacaacattt cgcagcagta tgaattatag tttgcaggaa
     3001 tatgccaacg atatcgttta tactattttg gaagtatgta attcggaaga aatcccccat
     3061 cccaatattg tcagcgaaag tgggcgcgcc atcgtagccc atcattccgt tttggttttg
     3121 gaaatttttg gtaatattga gaaaaaaccg tccgcattta aaatggattt ttctcccgaa
     3181 gatccggaca ttatacaaga aatgctggat ctgcgccatc aactgcacaa aaaaaacacc
     3241 aactatctcg aagtctatca cgatgccgca cagcgtcgcg aagaagcgca ctcgttattc
     3301 tcactcggtt atctgacgct tatcgaaaaa gctaaaatcg agaacctgta ttgggaaatc
     3361 atcaatatca tccgtgaata ttcccgcagt ctggaagtga tacccgaaga attggccgaa
     3421 cttgagccca aattggccga tcagtatctc tgcaattttt cgattttcca atcgttgctg
     3481 gaccattggg cgattgacca tttattcccc attgtaccga tacaccgact caacgaagag
     3541 ccgaccaaac gcgctattct agtggacatc acctgtgact ctgacggaaa aatctgtaaa
     3601 ttcattgaca tgaaagatgt caaagataaa ctggaactcc atccgctgaa tgatgatccc
     3661 tattatatcg gtattttctt attgggcgcc tatcaggata tcatgggtga tatgcacaat
     3721 cttttcggcc gcgtcaatga agtacacgtt tttatggatg acgaagaacc ggataactac
     3781 tacgtcgaag aaatcattcc cggttccaac gttgggcaag tattatcgct ggtgcagtac
     3841 aatgaaaatg agatcatcaa actcatcaaa aaaaccgtgg atcagcgcat caaagaaggg
     3901 caactcaaac ccagcgaagg tgttaaatta ttagacacct acgaagccgg acttaaagat
     3961 tatacctatc ttcagctcaa cggccgttca cgttagactg tggcggatat aggactatga
     4021 ttacacgcac ccgcattttg tatcttacgg ggccgttgct ttttttatta agttttcccc
     4081 cgcttcctac cggattttta gcctatatcg ctctccttcc tttttttgaa ttgctgcgcc
     4141 aaaaccaata ccggtccggt tttagaaccg gttacggctt gggtttgctt accatgggtg
     4201 tatttattca ttggctcaat gccaataccg gagcaacact accgcaggcc accggcatgt
     4261 atttcgggac gatcatgtat ctggctttgt actggggatg tttcggcgcc atacaaggtt
     4321 ttatccaacg caaacttggt gaacgtggtt tatggttagc tccctttttg tgggcatcgc
     4381 tggactatgt ccagtcgctc ggcgaacttg gttttacttg gcataatctg gcgacaaccc
     4441 agtcttatta tcttcctttg attcagttta tagatattac cggcatttac gggctttcgt
     4501 tttggatcgc tttggttaat gtcatcattt tcaaactaat caatgcctat cgtgaacaac
     4561 aaaaaaatca agcggtgtcc tatgctgtgg gtctgggaat gatttttctt ttgccgctag
     4621 tctatggctt tttgtcgtcg tggcgggaaa cctcggaaaa aaagttacgt gtagcgatta
     4681 tacaa
//
LOCUS       DAOQNQ010000253         4661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_7908_length_4661_cov_5.559488, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000253 DAOQNQ010000000
VERSION     DAOQNQ010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7908_length_4661_cov_5.559488"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..553
                     /locus_tag="PK754_12785"
     CDS             194..553
                     /locus_tag="PK754_12785"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNB10425.1"
                     /translation="MDKSSVKVNIYGSEYVIKGDDNADHIRAIADYVDQKMKEINKSG
                     AIKSPLKVAILAALNITDEFFKTSADQTKTIETMENKAQKMLDMLTQAPLPETLDLTP
                     EANEQSAAEEINLFSQK"
     gene            651..2213
                     /gene="rny"
                     /locus_tag="PK754_12790"
     CDS             651..2213
                     /gene="rny"
                     /locus_tag="PK754_12790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNB10426.1"
                     /translation="MEIAIFILAALVSAVAAFLFGQVYATKIAVAKRIREADEEAQQI
                     RKTAQSDAENLKKEKLIEVSDEWYKKRHEFDEQTKSTRIQIKNQQDELLKRERAVEQK
                     GDLLTRKEKDVDQKQQQVQTKLQEVTKKTEQLEAMIKNQNDKLETIARMTTEEAKQVL
                     MANMLEKAKEDAAKSIRNIKEQAQIQAKDQIKNIMLQAVQRTAMHHVTESTVTAFKLP
                     SNEMKGRIIGREGRNIRAFESITGCEIMIDDTPNTIMISGFDPIRREIARKTLEALIT
                     DGRIHPGRIEDVYEKARRDFDEHIMNTGEQALFEVGIHGAHVEIVKLLGKLKYRHQYG
                     QNLLQHSMETAAIAGMIASELGFDAQLAKRAAILHDIGFAADRSDQPHAVVGAELARK
                     FGENAVVQNAILFHHDDPMIAHPINVILYTANMLSKERPGAQKEVLNNYLKRLNKLEQ
                     IAMSFNGVREAFVVQAGREIRVMVDYATLDDNRAIQTADDIAQRIETEMEYPGQIKIT
                     VIREYRAVDIAK"
     gene            complement(2308..3414)
                     /locus_tag="PK754_12795"
     CDS             complement(2308..3414)
                     /locus_tag="PK754_12795"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HNB10427.1"
                     /translation="MKNYTHTTIPVRALASGQILNIDVFRFEGDPSVPSAYIQSGMHG
                     AELQGTAVIDFLIRFFTDHPPKGTVTLVPAANPYALNNKTGEYTQGRFDPTYGDNWNR
                     LYWDALNNEDGIDIENLVKTIRSDAQNSIQKILRHELIRKLEQQLQKPLTEMGYGRKL
                     ALTLQRMAVEADSVLDLHCASHSVRHVYAPEYARQTVRYLNIPYVLFIPNLFAGAMDE
                     AVFCPWWTLSQKLSVPCPVEAYTIELGNQEAYSAKDAHGDAMGIINYLVQRGTCDPIK
                     PKGRDSAESIFACRLQDYKTIFSPTGGLCEFSTVTGGIVPAQEPLAVILTKAGNEKIS
                     VALPYDCIPIVQYSSAALHQGVELLKVFSKWEVM"
     gene            complement(3416..>4661)
                     /locus_tag="PK754_12800"
     CDS             complement(3416..>4661)
                     /locus_tag="PK754_12800"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent decarboxylase"
                     /protein_id="HNB10428.1"
                     /translation="EKQRRVTTGRGVAMKALTAHHLTLLQEAADRVRKPFFAYDLDGL
                     RSHAMTLRSEGVRLWYACKANPLSAILRTMGACSFSADAASLGELRQILRYSGIAPKN
                     ILLTGPAKDEAFVTEALNAGVCTIIVESVHQLEMLQQLASTTHKPIQVLLRLQLDWDE
                     KSASVLGGSAITPFGLPPEVWQSVSWKSFPHCNVLGCHVFQWSNIGDIEKLNFFWHRI
                     AVESKRLMQHIGKPLEVLDLGGGLGVPYHADARELRWIEVDDALQKIRKQYEIPSIWL
                     ELGRYAVGGFGNYLTRVIDRKQVRGTELLILEGGVNHIARPALTHASFPVHTLKPSSA
                     KTQSFKLHGPLCTALDDLGLHDLPEDIQRGDWLVFRQCGAYGFTESMPYFLCHDIPAE
                     IIIENNQYAVVRESVPAENWLK"
BASE COUNT         1351 a         1140 c         1072 g         1098 t
ORIGIN      
        1 ctataatgcc cttcgccgtg aagcggatga attaaaacgc ggcaatgatg ctccgatcca
       61 ggctgtagaa tcgcgctctg aacccaaggc gctgcttgat ccggaattca gaacgcgcat
      121 gaaagtgcaa cttgagagtg ttttgaataa aataaacgaa ctggaaaaaa ttatttaatt
      181 ttttgcgata acagtggata agagttccgt caaagtcaac atctacggca gcgaatatgt
      241 catcaaaggc gatgataacg ccgatcacat tcgcgcaatt gccgattatg tagaccaaaa
      301 gatgaaagag atcaacaaaa gcggtgcgat caaatccccg ctcaaagtgg cgatccttgc
      361 ggcgctgaat attacggacg agtttttcaa aaccagcgcc gatcaaacga agacgattga
      421 aacgatggaa aacaaggctc agaaaatgct ggacatgctg acgcaggctc ccctgcccga
      481 aacgctggac ctgacacctg aggctaacga acaatcggct gccgaagaaa tcaatctttt
      541 ttcgcagaaa tagtcttcac tctgacatta tcccctgttg tcctttggcg cacagcgccc
      601 gaaggtcaca gggttttttt atagatcaac ggagttagat caagacaatc atggaaattg
      661 ctatttttat tttagcggcg ctggtatccg ccgtagcggc ctttcttttc gggcaagtgt
      721 acgcgaccaa aatcgccgtg gccaaacgca ttcgcgaagc cgatgaagaa gcccagcaaa
      781 ttcgcaaaac ggcccagtcc gatgcggaaa atctcaaaaa agaaaagctc atcgaagtca
      841 gcgatgaatg gtataaaaaa cgccatgagt ttgacgagca aaccaaaagc acgcgcattc
      901 agatcaaaaa ccagcaagat gaattactca agcgcgaacg tgccgtagag caaaaaggcg
      961 atttgctgac gcgtaaagaa aaagacgtcg atcagaaaca acaacaagtg cagacaaaac
     1021 ttcaagaagt caccaaaaaa accgaacaac ttgaagcgat gatcaaaaac cagaacgata
     1081 aactggaaac catcgcacgc atgacgacgg aagaggccaa acaggtgctg atggccaata
     1141 tgctggaaaa ggccaaagaa gatgcggcca aatccattcg caatatcaaa gaacaagcgc
     1201 aaatacaggc caaagatcag atcaaaaata tcatgttgca agccgtacaa cgcacggcca
     1261 tgcatcacgt gactgaaagc acggttactg catttaaact gccgagtaat gaaatgaaag
     1321 gccgtatcat cgggcgtgaa gggcgtaata tccgtgcctt cgaatccatt acgggttgcg
     1381 agatcatgat tgacgatacg cccaatacga tcatgatttc gggatttgat ccgatccgtc
     1441 gtgaaatcgc acgaaaaacg ttggaagcac tgatcacgga cggacgtatt catccgggtc
     1501 gtatcgaaga tgtgtacgaa aaagccagac gggattttga tgaacatatc atgaataccg
     1561 gagaacaggc gctttttgaa gtcggtatac acggcgccca tgtcgaaata gtcaaactgc
     1621 tcggcaaact caaataccgt catcagtacg gacagaatct cctgcaacat agtatggaaa
     1681 cggccgccat tgccggtatg atcgcatccg aattaggttt tgacgcacaa cttgccaaac
     1741 gcgctgcgat actgcatgat atcggatttg ccgccgaccg ctccgatcaa ccgcatgccg
     1801 tcgtgggcgc cgaactggca cgtaaattcg gtgaaaacgc cgtcgttcaa aatgccattt
     1861 tatttcatca tgacgacccg atgatcgccc accctatcaa cgtgattttg tacacggcga
     1921 atatgctgag caaagaacgt ccgggcgcac aaaaagaagt gctgaataac tatctgaagc
     1981 gcctcaataa attggaacaa attgcgatga gcttcaacgg cgtacgcgaa gcgtttgtcg
     2041 ttcaggccgg gcgggaaata cgtgtgatgg ttgattatgc gacactcgac gacaaccgcg
     2101 ctattcaaac ggccgacgat atcgcacaac gtatcgaaac cgaaatggaa tatcccggac
     2161 agatcaaaat cacggtgatt cgcgaatacc gtgccgtgga tattgcaaaa tagtattctc
     2221 tgacaattta aaagattata tccctcatcg gtattaaggc atgccaatca tccttgtttc
     2281 atgagggatt tttattgttg tccgaattca cattacttcc catttcgaaa acactttgag
     2341 caattcgacc ccttgatgca aagccgcaga tgaatattgt acgatcggga tacaatcata
     2401 gggtaatgcc accgatattt tttcattccc cgctttagtg agtataaccg ccagcggctc
     2461 ctgtgccgga acgatcccac cggtaaccgt actaaattca cacaatcctc cggtcgggct
     2521 aaagatagtt ttataatctt gtaaacggca tgcgaatatg gactccgcgc tatcacggcc
     2581 ttttggtttt atcgggtcgc atgttcccct ttgcactaaa taattaataa tacccatcgc
     2641 atcgccgtgc gcatcttttg cggagtaagc ttcctgattg ccgagttcga tcgtataggc
     2701 ttcaacgggg cacggcacac ttagcttttg acttaatgtc caccaagggc aaaataccgc
     2761 ttcatccatt gcaccggcaa aaagattggg aataaataaa acatacggaa tattcaaata
     2821 cctcacggtt tgacgcgcat actccggcgc atacacatgg cgtaccgaat gcgaagcgca
     2881 atgaaggtca agcacactat ccgcctcgac agccatacgt tgtaaagtca acgccagttt
     2941 gcggccataa cccatttccg taagcggctt ctgcagttgc tgttccagtt tacggatcag
     3001 ctcatgtcgt aaaatttttt gaatactatt ttgtgcatcg ctgcgaatgg ttttaaccaa
     3061 gttttctatg tctatgccgt cttcattatt caaagcatcc caataaagac gattccaatt
     3121 atcgccatac gtcggatcaa atcttccctg cgtatattct ccggttttat tatttagtgc
     3181 ataagggttg gccgccggga caagtgtcac cgtgcctttg ggcgggtgat ctgtaaaaaa
     3241 acgaattaaa aaatcaatca cggcggtacc ttgtaattcc gcaccgtgca tgccggattg
     3301 tatatatgcc gacggcacag acggatcacc ttcaaaacga aagacatcta tattcagaat
     3361 ttgacctgat gccaaagccc gtaccggtat cgtggtgtgc gtgtagtttt tcattttact
     3421 tcagccaatt ttcagccggc acgctttcgc gcaccacggc gtattgatta ttttctataa
     3481 tgatctccgc aggaatatcg tggcacaaaa agtacggcat actttccgta aagccgtatg
     3541 caccgcattg gcgaaacaca agccaatcgc cgcgttgtat atcttcaggc aaatcatgca
     3601 aacccagatc atccaaggcc gtacataagg ggccgtgaag tttgaaagat tgggttttcg
     3661 cagacgaagg cttcagagtg tgtacaggaa acgaggcatg cgtgagggcc ggacgggcga
     3721 tgtgattgac cccgccttct aaaatcagga gttccgtacc gcgtacctgc ttacgatcta
     3781 tcacacgtgt aagataattt ccaaatccgc ctacggcata acgtccgagt tctaaccaaa
     3841 tcgaaggtat ttcatactgc ttgcgaattt tttgtaacgc gtcatccact tcaatccaac
     3901 gcaattcgcg cgcatcggca tgatacggta cgccgagtcc acccccaagg tcaagcactt
     3961 ccaaaggttt gccgatatgt tgcatcaacc gcttagattc gacggcaata cgatgccaaa
     4021 aaaaattgag tttttctatg tcgccaatat tgctccattg gaaaacatga caacctaaca
     4081 cattgcaatg aggaaacgat ttccacgaca ccgattgcca aacttccggc ggtaatccaa
     4141 acggggtgat ggcgctgcct ccaagcacgc tggcgctctt ttcgtcccag tctaattgaa
     4201 ggcgaagaag aacctgaata ggcttgtggg tcgttgatgc aagctgctgc agcatttcaa
     4261 gttggtgcac gctctctacg atgatcgtac aaacgccggc attcaacgct tccgtgacaa
     4321 aggcttcgtc tttggccggg ccggtgagta atatgttttt tggtgcgatg cccgaatatc
     4381 ttaggatttg ccgtaattcg ccgaggctgg cggcgtccgc cgaaaaagaa cacgcaccca
     4441 ttgttcgcag aatggccgaa agcggattgg ctttacaagc gtaccacagg cgaacgcctt
     4501 ccgagcgtaa cgtcatagca tgcgaccgta aaccatccag atcgtaggca aaaaaaggtt
     4561 ttcgcactcg gtcggcagcc tcttgtaaaa gtgtaaggtg atgcgctgtc aatgctttca
     4621 tcgcaacgcc tcttccagtt gtaacgcggc gttgcttttc g
//
LOCUS       DAOQNQ010000254         4656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_7920_length_4656_cov_5.826777, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000254 DAOQNQ010000000
VERSION     DAOQNQ010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7920_length_4656_cov_5.826777"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1957
                     /gene="gyrA"
                     /locus_tag="PK754_12805"
     CDS             <1..1957
                     /gene="gyrA"
                     /locus_tag="PK754_12805"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HNB10429.1"
                     /translation="LLVNGSTGIAVGMATNIPPHNLTEVVDGLHALIDDPEITIKDLM
                     KHIKGPDFPTAGIINGVAGIKEAYETGRGKIIIRARANIETNKNNRQSIIITELPYTV
                     NKANLVEKISDLVREKKVEDISALRDESDRDGIRVVIELKRDANAQVVMNNLFKHTQM
                     QTTFGIIILALVDGRPMVLNLKQTLQHFIDHRHEVVQRRTQFELEAAERRAHILEGLK
                     IALENIDAIIQLIKKSKDPAVAKEGLMKKYKLSEIQATEILKMQLQRLTNLEVEKIEN
                     EYRDVIKLIEKLKGILASKAKRMAIIKDELADIRKTFGDERRTEILKKEVEEIDFEDT
                     IAEEEVVVTISRQGYIKRFPVSGYRRQGRGGQGVSGGQTKDDDFIEHLFVASTHEYIM
                     FFTDKGRCYWLKVYEVPELSKTARGKHISNLIEQQSDEKIRAVINVKEFSDQLSVLMV
                     TKEGVIKRTNLSEYSNPRKGGIIAITIEKNDDLVDAQLTDLKQDVLLGTHDGIAIRFH
                     GEEVREMGRSARGVTGINLEKGDYVVGMVSLKRENGTILVVSDMGYGKRSEVGDYRTT
                     RRGGKGIISMKATDKTGAMIAIREVVDNEDLMIITQNGMVIRLKMADIRTMGRNTQGV
                     RLVKLKEDDTISDITSIKEEESEEAQD"
     gene            2042..3475
                     /locus_tag="PK754_12810"
     CDS             2042..3475
                     /locus_tag="PK754_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10430.1"
                     /translation="MFETIVDGIYEQIRQKMHTDQNHWSYFELTGKGSDEVVRKIFTG
                     KYEQIFRAEKARIVAARINTALPYGKAPVDKALDILVDELKREWLIPKTEVEQTLRES
                     LLLRLQYITMPLATVESLMFADTPARTIEEIVNQYRAFEIYKYYPDALERYAKAKAIT
                     HLNKIQLRLLINEINQSLFGKDALENVLKLCGLVMKEVNELRGASTNTIEIGLLMKAF
                     ADRSLRDFEVALNIEKELGNEQINIYGLRQVLNRFVILKEKKAVAAEKTIVNLAPKSE
                     PSPVESRLAVKPKDTTDLVIDKVRKDEESGEIIDLNAILSHQPSKEKTDELRPKPKEP
                     TVETIAFPADMKAKTGSTKIKFAPETVEEVTGEFIKEELVQGFSDDTQTIRLKDIKES
                     DNLVLLQSVITPKDEQIFMKSIFGGDEAYYRDFVSQVNRSKTWKEAITVVDDMLFEKK
                     VDPYCKEAIRLSDVVYMRYYPPEKNNG"
     gene            3488..3634
                     /locus_tag="PK754_12815"
     CDS             3488..3634
                     /locus_tag="PK754_12815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HNB10431.1"
                     /translation="MENLGYLFAAFAAIWVLLFYYVQRMNSKQKQMMKDLEALRQIVE
                     EKKG"
     gene            complement(3644..4387)
                     /locus_tag="PK754_12820"
     CDS             complement(3644..4387)
                     /locus_tag="PK754_12820"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNB10432.1"
                     /translation="MQLRVASITDRGLNPSRTHNEDSFFTGNQLYIVADGLGGELAGE
                     VASQMTIEKFNEWFPDATRESANEAGSELAAIERTVKRVNAYVYQEAQKPEFKNMGST
                     LSILYFFRNHALIGHVGDSKIYRVRKGKTEQLSHDQSMVQQLLDKGIITTEQAKRHPM
                     RNVLLSCIAHKPDIDVFTYDTDIQNGDFYLLCSDGVSEYVGHGAIAEMAGNGFSGEEI
                     CATIKNIVYAGGAMDNMTAIVIFVDQVDA"
BASE COUNT         1362 a          997 c         1147 g         1150 t
ORIGIN      
        1 tttgctggtc aacggttcta ccggtatcgc tgtcggtatg gcgaccaata ttcctccgca
       61 taatctgacg gaggtggtgg acggattaca tgctttgatc gacgatccgg aaatcacgat
      121 caaagatttg atgaagcata tcaaaggacc ggatttcccg acggccggca ttatcaatgg
      181 cgtggcgggt atcaaagaag cctacgaaac gggccgcggt aaaatcatca tccgtgcgcg
      241 cgccaatatc gagaccaata aaaataatcg gcaaagcatt attatcaccg aactcccgta
      301 cacggtgaat aaagcgaatc ttgtcgaaaa aatttccgat cttgtgcgtg agaaaaaagt
      361 cgaagatatc agcgctctgc gcgacgaatc cgaccgcgac ggtatccgcg tagtgatcga
      421 actgaagcgt gatgccaatg cgcaagtggt gatgaataac ctcttcaagc atacgcagat
      481 gcaaacgacg ttcggcatca tcattctggc gcttgtggac ggacgcccga tggtcctcaa
      541 tcttaagcaa acgcttcagc atttcattga ccatcgccac gaagtggtgc aacgtcgtac
      601 gcaattcgaa ctcgaagccg cagaacgtcg ggcgcacatt ttagaaggtt tgaaaatagc
      661 acttgagaat attgatgcga tcatccagct gatcaaaaag tccaaagatc ccgctgtggc
      721 taaagaaggc ttgatgaaaa aatacaagct ttccgaaatt caggcgacgg aaattctcaa
      781 aatgcagctc cagcgcctta cgaacctgga agtcgaaaaa atcgaaaacg aataccgcga
      841 tgttatcaag ctcattgaaa agctcaaagg tattctcgcc agcaaagcca aacgtatggc
      901 gatcatcaaa gatgaattgg cggatatccg taaaaccttt ggcgatgaac gccgtacgga
      961 gatactcaaa aaagaagttg aagaaattga ctttgaagat acgatcgctg aagaagaagt
     1021 ggttgtcacc atttcgcgcc aggggtatat caagcgtttc cctgtgagcg ggtatcgccg
     1081 ccagggccgc ggaggtcaag gcgtgtccgg cggtcagacc aaagatgatg attttatcga
     1141 gcatcttttt gtcgcttcca cccacgaata catcatgttc tttactgaca agggccgttg
     1201 ttattggctg aaggtttatg aagtgcctga acttagtaaa acagcgcgcg gtaaacacat
     1261 cagtaacctg atcgaacagc aatcggatga gaaaatccgt gctgtgatca atgtcaaaga
     1321 gttcagcgat caattgtccg tgttgatggt aaccaaagaa ggcgtcatca aacgtaccaa
     1381 tctttccgaa tacagcaatc cccgcaaagg cggtatcatc gctattacca tcgagaaaaa
     1441 cgatgatctt gtagacgctc agttgacgga tcttaagcaa gatgtattgc tcggaacgca
     1501 tgacggcatc gctattcgtt tccacggcga agaagttcgt gaaatgggtc gctcggcgcg
     1561 cggtgtcacc ggtatcaatt tggaaaaagg tgattatgtg gtcggcatgg tttcactcaa
     1621 acgtgaaaat ggtacgatcc ttgtcgtttc cgatatgggt tatggcaaac gcagcgaagt
     1681 cggcgattat cgtacgacac gccgcggcgg aaaaggtatc atctcaatga aagccaccga
     1741 caaaaccggg gccatgatcg ctatccgtga agtggtggac aatgaagatc tgatgatcat
     1801 cacacaaaac ggtatggtca ttcgtctcaa aatggcggac attcgtacca tgggacgtaa
     1861 tacccaaggc gttcgtctgg tcaaactgaa agaagacgac acgatttcgg atattacaag
     1921 tatcaaagaa gaagaatcgg aggaggcgca ggactgatca ggcactgcgc aagctgtcag
     1981 tagttctgac ggcacatcat aataaagggc tcccgcgcgc ggtgcgcggg tgaaacgggg
     2041 tatgtttgaa acgatagttg acgggattta tgaacaaatc cgtcagaaga tgcatacgga
     2101 ccagaatcac tggtcgtatt ttgaactgac cggaaaaggt tcggatgaag tggttcggaa
     2161 aatttttacc ggtaagtatg aacagatttt tcgagcagaa aaagcccgca tcgtggcggc
     2221 gcgtatcaat acggcccttc cttacggcaa agcgccggta gataaggcgc tggatatatt
     2281 ggtggatgaa ctgaaacgcg aatggctgat accgaaaacg gaagtggaac aaacacttcg
     2341 cgagtcgtta ttgttacgtt tgcaatacat cacgatgccg ctggcaacgg ttgaatcgtt
     2401 gatgtttgcc gatacgccgg cgcgtacgat cgaagaaatc gtcaatcagt accgtgcatt
     2461 tgaaatctac aaatactatc ccgatgcact ggagcgttat gctaaagcca aagccatcac
     2521 gcatctcaat aaaatccaac ttcgtctgct gatcaacgaa atcaatcaaa gcctttttgg
     2581 taaagatgct ctggagaacg tgctcaaact gtgcggtctt gtgatgaaag aagtgaatga
     2641 gctgcgcggc gcgtcgacaa atacgatcga aattggtttg ttgatgaagg cttttgccga
     2701 tcgttcgttg cgtgattttg aagtagcgct caatatcgaa aaggaattgg gcaatgagca
     2761 gatcaatatt tacggcttac ggcaagttct caatcgtttt gttatcctga aagagaagaa
     2821 agccgtggcg gccgaaaaaa cgatcgtgaa tctagcgcct aaatcggagc cctcaccggt
     2881 cgaatccaga cttgcggtta agcccaaaga tactacggat ctcgtcatcg ataaagtgcg
     2941 taaggacgaa gaaagcggcg agattatcga cctcaatgcg atattgagcc atcaaccttc
     3001 aaaggaaaaa accgacgaac tgcgccccaa accgaaagag ccgactgttg agacgatagc
     3061 ttttcctgca gatatgaagg ctaaaaccgg ttcgactaaa ataaaatttg ctcctgaaac
     3121 agtcgaagag gtaacgggtg aatttatcaa agaagagctg gtgcaaggtt tttcggacga
     3181 tacgcaaacc atccgtctta aggatatcaa agaatcggat aatttggtgc ttttgcaatc
     3241 ggtgatcacg ccgaaagatg aacagatttt tatgaagtct atttttggcg gcgatgaagc
     3301 gtactaccgt gattttgtca gtcaagtcaa tcgcagtaaa acgtggaaag aggcgatcac
     3361 ggtggtggat gatatgctgt tcgaaaagaa agttgatccg tattgtaaag aagcgattcg
     3421 attgagcgat gttgtatata tgcgttacta tccgcctgaa aaaaataacg gttaaggaat
     3481 aatttcgatg gagaatttag gttacttgtt tgccgcattt gccgcgatat gggtattgct
     3541 gttctactat gtgcaacgta tgaatagcaa acaaaaacag atgatgaaag acttggaagc
     3601 gttgcggcag atcgtggaag aaaaaaaagg ttagcggagt tttttacgcg tcaacctgat
     3661 cgacaaatat gacgattgcc gtcatattgt ccatggcgcc gccggcatac acgatattct
     3721 ttatagtggc gcaaatttct tctcctgaaa atccgttgcc cgccatctcg gcaatagccc
     3781 cgtgaccgac atactcggac acgccatccg agcataacaa ataaaaatcc ccgttctgta
     3841 tatcggtgtc atacgtaaag acgtcaatat ctggcttgtg cgcgatgcag ctcagtaaaa
     3901 cattgcgcat cggatgacgt ttagcttgtt cggtggtaat aataccttta tccaaaagct
     3961 gctgcaccat gctctgatca tggctgagtt gttcggtttt ccctttgcga acacgataga
     4021 tcttgctgtc accgacatga ccgatcagcg catgattcct gaaaaaatat aagatcgata
     4081 aggtggaccc catattttta aattcgggct tttgtgcttc ctgatacaca taagcgttga
     4141 cccgtttgac cgtgcgttca atagcggcga gttcactgcc agcttcattc gccgattccc
     4201 gtgtggcatc ggggaaccat tcgttgaatt tttcaatcgt catctgactg gccacttcgc
     4261 cggccaattc tccgccgagc ccgtcggcga ctatataaag ctggtttcct gtaaaaaaac
     4321 tgtcttcatt gtgtgtgcga ctgggattga gcccccgatc ggtgatactg gctacacgta
     4381 actgcatagg tgatcgttaa attccgtttt ttagtgagag caatttgaaa taatagaaaa
     4441 aagagaatgg ataatcaaga attcactttg aattcaataa tttgttttga ctctttcgtt
     4501 aacggaagtt attatttgac aatcaaacta aggtaaaaga ggtgacttca tgaaagcgtt
     4561 gtatttattt ttgatgtcag tagttttgac gggtattgtt tccgcacagg ataaatccta
     4621 caatacagat caattgaagt cggctaaacg catttc
//
LOCUS       DAOQNQ010000255         4653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_7929_length_4653_cov_4.573728, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000255 DAOQNQ010000000
VERSION     DAOQNQ010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7929_length_4653_cov_4.573728"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PK754_12825"
     CDS             <1..274
                     /locus_tag="PK754_12825"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNB10433.1"
                     /translation="HIVENASLRTTIETLLKKKYTVLAADMHGEKIPPKMLAPWALII
                     GSEAHGLSDEVRSAPIQLMAIEKYGRGESLNAAVSCGILLHALTRS"
     gene            354..1679
                     /locus_tag="PK754_12830"
     CDS             354..1679
                     /locus_tag="PK754_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxyadenosine deaminase"
                     /protein_id="HNB10434.1"
                     /translation="MALTLIKNGTVITMNSARDIVKADILIDGAIIRKIGKIRVPSGS
                     RVRVIDAQGCIVAPGFVQTHIHLCQTLFRNLADDLELLDWLKLKIWPFEAAHNPASIR
                     LSAEMGLTELIRGGTTTIMDMGTVHHTDHIFEAIDRSGIRALCGKAMMDDCPGAPKGL
                     IETRAWSVEESLHLMRYWHRRSDGRLRFAFAPRFVLSCSEELLCEVRDLSEEYGLLVH
                     THASENRGELAEVIKKCGTPNVEYFHDIRLTGERLCLAHCIHLNETERHILRDTGTKV
                     MHCPSSNLKLGSGIAHVPELLGLGVTVSLGADGAPCNNNLDMFQEMRLAALLQKPRLG
                     PRSITAEEVYTMATLGGAKTLGLSDEIGSLETGKRADLIIMQLDEIHSRPEENLYSQI
                     VYSAKSTDVRTVVVDGKVLMKDRRMVTLDDESVRHRIGPELKKLLNRIA"
     gene            1716..2081
                     /locus_tag="PK754_12835"
     CDS             1716..2081
                     /locus_tag="PK754_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10435.1"
                     /translation="MYNVLEVITADKGIMSEYFILSCIEFFILLIVSERVLAARNGWA
                     NPSIWRDNTHIRMCYLIRKWSIPSLFLILNTVAVSSFAHHAHELAELFFMNVLVLMSS
                     AVLGLLLGESAVQRYRVGR"
     gene            complement(2078..2863)
                     /locus_tag="PK754_12840"
     CDS             complement(2078..2863)
                     /locus_tag="PK754_12840"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNB10436.1"
                     /translation="MNHDWDNIKNWSLARGNSLTTGYTIITLDAIGSTNEYLKQLWRD
                     EKAQHGCLVIADTQTAGKGRLGRTWESPTGAGLWMSLLIKPSLPPDKHFAYSFAAAVA
                     VAEAIEQTIRLRPELKWPNDVLLCQKKCCGILLESVTTGSEPWIIVGIGINVTQESFP
                     DALSDTAISLQQAHAAPVHRLQLLQNLIPALQSNFSSDSREILIKWQSYCRMFGAMVS
                     VFQQDSVMEAKAVGLAPDGGLVVENEGKRIKVYAADVKIRLKN"
     gene            complement(2853..3638)
                     /locus_tag="PK754_12845"
     CDS             complement(2853..3638)
                     /locus_tag="PK754_12845"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HNB10437.1"
                     /translation="MKGFLHAALTALERYNRIAIATVVRASGSTPRAAGAKMLIFPDG
                     TAQFTIGGGLFESLVIKDALEVLQSGKPLTRTYAFTLDGQHAIGAVCGGSVEVLIEAM
                     HNTPQLWIIGGGHIGQALMRAAAPLHFTISLFEDRAEYARTPHDLPHVRAHHVAADYH
                     DMPEPGDDTFVCIVTKGYITDEAALRRVIRSRAHYIGFLGSRRKIHTVFENMKRDGFE
                     GKLFERIYAPIGESIHADAPEEIAISILAQIIRVKNQVFTVES"
     gene            complement(3635..>4653)
                     /locus_tag="PK754_12850"
     CDS             complement(3635..>4653)
                     /locus_tag="PK754_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10438.1"
                     /translation="PVERFFHYIMDTGSIEQTVRQSGLYGKLIQQATRNVTQHRQFKK
                     LESTALALSTEEVQRLMTKTFAEDWFYTSLRTAHLDLINPLNAEPSQNDHVGYIIITD
                     KKDLLLENLAQIIENKMNALPECSPRQTLTLAGQYMRNNGKITLDKIKLDCKPPQSLA
                     SEVRTIIRQEVSQGLTALPDTIYYLQWMDEKNSKMQHKDFIEAGTTMRNVRLGSIILL
                     FAGMLILVVINFNNKKQLLVRTALPLILTGLLLGGGVWFGIRYFEDPAHAEIFKIEGP
                     AAINKQPMSTDTANIILGVVKTFFLDYMRSIFYTCLVIGGIGLMMLVSSRYVHDEHME
                     SGAS"
BASE COUNT         1127 a         1123 c         1204 g         1199 t
ORIGIN      
        1 gcatatagtg gaaaatgctt cactccgtac aacgatcgaa acgttattga aaaaaaagta
       61 caccgttttg gcggcggata tgcatggcga aaaaataccg ccgaagatgc tcgcaccctg
      121 ggcgctgatc atcgggagcg aagcgcacgg attgtccgat gaagttcgat ccgcgcccat
      181 tcagctgatg gccatcgaaa aatatggccg cggtgaatcg ttgaatgcgg cagtatcatg
      241 cggcattttg ctccatgcgc ttacacgatc ataaaaacaa accggcttgt catctcagac
      301 tccgtttctg agtattcaaa agagctaatc cgacaagcac ataaataaaa attatggcat
      361 taacacttat caaaaacggc actgtgatta cgatgaattc cgcccgggac atcgtcaaag
      421 ccgatattct cattgacggc gccatcattc gcaagatcgg aaaaattcgc gtgccgtccg
      481 gatcccgcgt gcgggtcatt gatgcccaag gctgtatcgt tgcgcccggc ttcgtacaga
      541 cacatattca cctttgtcag accctgttcc gtaatttagc cgatgatctc gaactgcttg
      601 attggctcaa gcttaaaatc tggcctttcg aagcggcgca taacccggct tccatccggc
      661 tgtcggcaga gatggggtta acggaattga ttcgcggagg cacgacgacg atcatggata
      721 tgggcacggt gcatcatacg gatcatattt ttgaagcaat tgaccgttcc ggaattcgtg
      781 cgttgtgcgg caaagcgatg atggacgact gtcctggtgc gcccaaaggg ttgatcgaaa
      841 cgcgggcatg gtctgtcgaa gagagcctgc atctcatgcg atactggcat cggcgctcgg
      901 acgggcgcct acggtttgcc ttcgcgccgc gctttgtgct ctcgtgcagt gaagaattac
      961 tttgcgaagt gcgtgactta tccgaagaat acggcctgct ggtgcatacg cacgcgtctg
     1021 aaaaccgcgg cgaactggcc gaagtgatca aaaaatgcgg cacgcccaat gtcgaatatt
     1081 ttcatgatat acgcctcacc ggcgagcggc tgtgccttgc gcattgcatt cacctcaatg
     1141 aaacagaacg ccatatcctg cgtgataccg gcaccaaagt gatgcattgc ccttcgtcta
     1201 atctcaaact cggctcgggc atagcccatg ttcccgaatt gctgggtctt ggtgtgacgg
     1261 tatccttagg cgcggatggc gctccgtgca ataataattt ggatatgttt caggaaatgc
     1321 gactggctgc acttttgcag aaaccgcgtc tcggcccgcg ttcgatcacg gcggaagaag
     1381 tgtatactat ggccactttg ggcggtgcaa aaacattggg tttgtcggat gaaatcggaa
     1441 gccttgaaac cggcaaacgc gccgatctga tcatcatgca gttggatgaa attcactccc
     1501 gcccggagga aaacctgtat tcgcaaatcg tctactcagc caaaagtacg gatgttcgta
     1561 cggtcgtggt ggatggaaag gtgctgatga aagaccggcg catggtaact ttggacgatg
     1621 aatcggtgcg ccaccgcatc ggacctgaat tgaaaaaact attaaatcgg attgcctaaa
     1681 aagttaaaaa aaacttaacc ggaaaggtac atcgcatgta taatgttctt gaagttatca
     1741 cagcggataa aggcattatg agcgaatatt ttatcctatc ctgtatcgaa ttttttatct
     1801 tactgatcgt atccgagcgg gttttagcgg cccgcaacgg atgggccaac ccctcgatct
     1861 ggcgcgacaa tacacacatt cgtatgtgtt atctcattcg taagtggtct atcccttcat
     1921 tatttctcat actcaatacg gtagccgttt cgtcgtttgc acaccatgct cacgaactgg
     1981 ccgaactgtt ttttatgaac gtgttggtat taatgtcatc ggcggtgctg gggcttttgt
     2041 tgggagaatc tgctgtgcaa cggtatcgcg tagggcgtta gttttttaaa cgtattttga
     2101 catcggccgc atatactttt attcgttttc cctcattttc cacgaccaat cctccgtccg
     2161 gagctaagcc gacggcttta gcttccatca cactatcttg ctgaaacacg ctgaccatgg
     2221 cgccaaacat gcggcagtag gattgccatt tgatcaaaat ttcacggctg tctgagctga
     2281 aattagattg tagtgcgggg atcaaatttt gtaataattg aaggcgatgt acgggtgcgg
     2341 cgtgagcctg ctgaagcgag atcgccgtat ccgataaggc gtcgggaaaa ctctcttgcg
     2401 tcacattgat gccgatgccg acgataatcc atggttcaga accggttgta accgattcca
     2461 gtaaaatgcc gcagcatttt ttttgacaaa gtaaaacatc attgggccat ttgagctcgg
     2521 ggcgaagacg tatagtctgt tcgatggctt cagcgacagc gacagccgct gcaaaagaat
     2581 aggcgaaatg tttatccggc ggcagcgacg gtttgataag cagggacatc cataaacctg
     2641 caccggtcgg tgattcccaa gtgcgcccca ggcgtccttt gccggccgtc tgggtatcgg
     2701 ctatgacgag gcatccgtgt tgcgcttttt catcacgcca gagttgtttg aggtattcgt
     2761 tggtcgaacc gatcgcgtcc agtgtgatga tggtgtaacc ggttgtaagt gagttgccgc
     2821 gggcgagcga ccagttcttt atgttatccc agtcatgatt caacggtgaa tacctgattt
     2881 tttacacgaa tgatctgcgc aagaatactg atcgcgatct cttccggagc gtcggcatga
     2941 attgactcac cgatcggggc gtaaatacgt tcgaataatt tgccttcaaa tccgtcgcgc
     3001 ttcatgtttt caaaaacggt gtggatcttg cgtcggctgc cgagaaagcc gatgtagtga
     3061 gcacggctgc gaattacgcg acggagtgcg gcttcgtccg tgatataacc tttggtgacg
     3121 atgcacacaa acgtgtcatc gcccggctcg ggcatatcat gatagtcggc cgcgacatga
     3181 tgtgcgcgca cgtgcggcaa gtcatgcggc gtacgtgcgt attcggcacg atcttcgaaa
     3241 agactgatag tgaaatgcag tggcgccgcg gcgcgcatca gagcttgccc gatatggccg
     3301 ccgccgataa tccaaagttg aggcgtattg tgcatcgctt cgattaaaac ctccacactt
     3361 ccgccgcaca cggcgccgat ggcatgctga ccgtcgagcg tgaacgcata cgtgcgggtt
     3421 aagggtttgc cggattgtaa aacctctaaa gcgtctttga ttaccagaga ttcgaaaagt
     3481 ccgccgccta tagtaaactg tgccgtgcca tccggaaaaa tgagcatttt ggcgccggcg
     3541 gcgcgcggtg tagagccgct ggcgcgtact acggtagcga tagcgatgcg gttataccgt
     3601 tctagcgctg tcagcgcggc atggaggaaa cctttcatga ggcgccgctc tccatatgtt
     3661 cgtcgtgcac atagcggctg ctcaccagca tcatcaggcc gataccgcca atgaccaagc
     3721 aggtgtaaaa aatactgcgc atgtagtcca gaaaaaatgt ttttaccaca cccaatatga
     3781 tattggctgt gtcggtgctc ataggttgtt tattaatcgc cgcaggcccc tctatcttaa
     3841 atatttccgc atgggccggg tcttcaaaat atcggattcc aaaccatact ccgccaccca
     3901 ataaaagtcc tgtgagtatc agaggtaaag ctgtgcgtac gaggagttgt tttttgttgt
     3961 taaaattgat caccacgagt atgagcattc ctgcaaaaag caggatgata ctgcctaagc
     4021 ggacattgcg catcgtagtg cccgcttcaa taaagtcctt atgctgcatt ttcgaatttt
     4081 tttcatccat ccactgtaaa tagtagatcg tatccggtaa cgccgtgagt ccttgtgaaa
     4141 cttcctggcg tatgatagtg cgcacctccg aggcaagtga ctgcggcggt ttacagtcca
     4201 gttttatttt atccagagta attttgccgt tattccgcat atactgtccg gctaaagtaa
     4261 gggtttgccg gggtgaacat tccggcaaag cattcatctt gttttcgatg atttgtgcca
     4321 gattttcgag caacaaatct tttttatcgg tgatgatgat gtaaccgaca tgatcatttt
     4381 gggaaggttc tgcattgagc gggttgatca gatcaagatg cgccgtgcgg agggacgtgt
     4441 aaaaccaatc ctcggcaaag gttttagtca tcagccgctg tacttcctcc gtgcttaacg
     4501 ccagcgccgt ggattcgagt tttttaaact gccgatgctg cgtcacatta cgtgtagcct
     4561 gttgtatcag tttaccgtac aaaccggatt gacgtacggt ttgttcgata ctgcccgtat
     4621 ccatgatata atgaaaaaac cgttcgacgg gga
//
LOCUS       DAOQNQ010000256         4644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_7951_length_4644_cov_6.070386, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000256 DAOQNQ010000000
VERSION     DAOQNQ010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7951_length_4644_cov_6.070386"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PK754_12855"
     CDS             <1..250
                     /locus_tag="PK754_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HNB10439.1"
                     /translation="FGPEIQSVIGRIIRRSVEYAFSHPTSSKSYVKHHAQELDDTVIA
                     EHIKLYVNDYTLDLGTKGYAAVDFLKKEALRIGILPSS"
     gene            299..592
                     /locus_tag="PK754_12860"
     CDS             299..592
                     /locus_tag="PK754_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10440.1"
                     /translation="MSANLKKTNSTKLQQAKKSPVKKATLPFTAENYYIFFAGIITII
                     IGYVCLATPPVYGTVSMTIAPILLVLGYLVIIPVAILYRKKEEVQSIESTEGI"
     gene            594..920
                     /locus_tag="PK754_12865"
     CDS             594..920
                     /locus_tag="PK754_12865"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNB10441.1"
                     /translation="MRLTILTLAAILISSCGKEEKLDKAVVKVNSAICGECSGTITKA
                     VMAVNGVKKVEVDTDTKIATISYVPGSVKIGDIESAIVKSGYDANDQKADPAAYEQLP
                     DCCKKK"
     gene            1070..2428
                     /locus_tag="PK754_12870"
     CDS             1070..2428
                     /locus_tag="PK754_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10442.1"
                     /translation="MKSFVKYFFETNASEVTESKKGLHISLSKPLRDVFGKEKLDLVF
                     DTKDLDESADLVTHGSYVLNTIYEFLQETGTKAVSRLKEKHEISRESIARQIHIQNAS
                     INHVKTKKQKVADILFNFRIGYLSDEKSEDIFTLGIDHRGHVFDARSYYTDEVIKHDL
                     ETLSNKSGIEISQKEIEVAFRTCLKEVSTRAQEHGQGLQKEILKRLHRNVTRIKGYYT
                     AQIDEMHRSQTSYEEKRLTIEREYQHKLKEEIENHKLRIVLKLLSYHIIERTEIEVTT
                     TVEHNKTGHKAEVPLLYDSYHGLLDYGHCPSCKLPIQNMTLLADGKIGCSRCVFVCSG
                     CKDEFPDKEHAHHCMVCNTELCTNCNTHCHDCGHTVCETHSTVCFADKEIVCDQCVKS
                     CSDCGRTMCSDHTFLCHATKVPICHEHRVICPKCRKIYARGFIDKQKKNDRKCPECLT
                     TL"
     gene            complement(2485..3228)
                     /locus_tag="PK754_12875"
     CDS             complement(2485..3228)
                     /locus_tag="PK754_12875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNB10443.1"
                     /translation="MTNIEVIEGYGRENYGSFVLKKVYQKNVSTGLIIGITLHIAGIM
                     TYYISTVVFKEEQIRMVRITDINELQSAPPLQEQTAAPKVKVEVQEVIKPVAGIPIVV
                     PDAEAKPDMTIQTQEEIKTDIAAPTSFGSDGGEVKVDISGTIGDIGGGEPGMDEFVPV
                     EQQPVIVAKVQPVYPELAKKAGLEGKVIVKALIDESGTVTKCVVLQGDEMFKESAVNA
                     LMSMKFKPAINGNRPVKVWITYPFTFRLK"
     gene            complement(3225..3803)
                     /locus_tag="PK754_12880"
     CDS             complement(3225..3803)
                     /locus_tag="PK754_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNB10444.1"
                     /translation="MAEVGGAQPRSHGKGKKKKKARTGVRLDMTPMVDVAFLLLTFFM
                     LTTAFRLPQTMEISIPEDDKPENNVKIAESNIMQIRIDLDNNIFWNQGNEKPQKATMK
                     ELREIIIERSEKNIANGKGDTIKDKFKLIVLIKIDKRAKYEHMVNIVDELNLAMFDLN
                     QKNNLTEANRLSPRFAFAPFSEQDQKEVAKAQ"
     gene            complement(3822..4349)
                     /locus_tag="PK754_12885"
     CDS             complement(3822..4349)
                     /locus_tag="PK754_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNB10445.1"
                     /translation="MAKHKKHRVGVKLDMTPMVDVAFLLLTFFMLTTTFRPPEEISVT
                     VPDSHSQLKLPSANVITLSVAKDPIILKKGTQNEENTGTRIWMDVDAQVVRGKIFGAD
                     YAQSAGKAVKLDELQNLITKARLENLAIRGASGAMRVVLKADKDADYGVVSEIIDILQ
                     KSKITTVNFITNLEK"
     gene            complement(4372..>4644)
                     /locus_tag="PK754_12890"
     CDS             complement(4372..>4644)
                     /locus_tag="PK754_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNB10446.1"
                     /translation="STMVGLLGTTLGMIRAFKALAQSGAPDAVSLSLGISEALINTAG
                     GLGAAIVAIVAYNYFVNKVDTYTYLIDEASYNVVEILTTTHGDDKN"
BASE COUNT         1312 a         1051 c          968 g         1313 t
ORIGIN      
        1 gtttgggcca gaaatccagt cagtgattgg acgcattatt cgacgcagcg tggaatatgc
       61 tttttcccat cccacgtcga gtaaatctta cgtcaaacat cacgctcaag agcttgacga
      121 cacagttatc gcggaacaca tcaaactcta cgtcaacgat tatacccttg atctgggaac
      181 taaaggttac gcggcggtag atttcttaaa aaaagaagcg cttcgtatcg gcattctccc
      241 ctcatcttga cattcgcctg ttgaatatct atattgctgg ccaacaaaag gaaaaaatat
      301 gtctgctaat ctcaaaaaaa caaattcaac caagcttcaa caggccaaaa aatctcctgt
      361 taaaaaagct accttacctt ttaccgctga aaattattat atattttttg ccggtattat
      421 tacgatcatc atcggttatg tttgccttgc aacaccgccg gtttacggta cagtttcgat
      481 gaccatagca cccattttgc ttgttttagg ctatttggtg ataatccccg tcgctatttt
      541 gtatcgcaaa aaagaagaag ttcaatccat cgaatcaacg gaaggaatat aacatgcgtt
      601 tgaccatctt aacattagcg gccatcttga tttcttcttg cggaaaagag gaaaaattag
      661 ataaagccgt agtaaaagtt aattccgcga tctgcggcga atgctcgggt accattacaa
      721 aagccgtgat ggccgttaat ggtgtcaaaa aagtagaggt ggacacggac acaaaaatcg
      781 ccaccatctc ttatgttccc ggttccgtca aaatcggtga tatcgaatcc gcaatcgtca
      841 aatccggata tgacgccaat gatcaaaaag ccgatccggc agcctatgaa caattacccg
      901 actgctgtaa gaaaaaataa tactttttaa cgcgatttag ggagcgtttc caaaaggttc
      961 tcttttacgg taaaaacctt cgcatgatcc gttcgttcat aatgacaacg gatcatgcga
     1021 aaggcactaa gtaagcacct tatggaatag ctcccttttt ttattaagca tgaaatcatt
     1081 cgtaaaatac ttttttgaaa cgaacgcatc ggaagttaca gagtccaaaa aagggcttca
     1141 catttcgctt tcaaagcctc tgcgcgatgt ttttggaaaa gaaaagctag atctggtttt
     1201 tgatacaaag gacctggatg aatcggctga cctggttacc cacggcagtt atgtacttaa
     1261 tacgatatac gagtttttac aagaaaccgg aacgaaagcc gtaagtcgtc ttaaagaaaa
     1321 gcatgaaatt tcccgcgaaa gtatcgctcg ccaaatacac attcaaaatg cgagcataaa
     1381 ccacgtcaaa actaaaaaac aaaaagtagc cgatatactt tttaattttc gaatcggtta
     1441 cctgtccgac gaaaaatcag aagatatctt cacattggga attgatcacc gagggcatgt
     1501 tttcgatgca cgaagttact ataccgacga agtgataaaa catgatctcg aaactctgtc
     1561 caacaaaagc gggattgaaa tttctcaaaa agagatcgaa gtcgcttttc gcacctgcct
     1621 caaagaggtt tcaacccgcg cacaagaaca cggtcaaggt ttacaaaaag agatactaaa
     1681 acgcctgcat cgcaacgtca cgcgcatcaa aggttattat acagcccaaa ttgatgaaat
     1741 gcatcgcagt caaaccagtt atgaagaaaa gcgtctcaca attgaacgag aatatcagca
     1801 caaacttaag gaagaaattg aaaaccataa gttgcgtatc gttctaaagt tactcagtta
     1861 tcatattatc gaaagaactg aaatagaagt cacgacaacg gtcgaacaca acaaaaccgg
     1921 ccacaaagct gaggtgccgc ttttgtatga tagctaccac ggattattgg attacggtca
     1981 ctgcccttct tgcaaattgc cgatacaaaa tatgactctt ttggctgatg ggaaaatagg
     2041 ttgctcgcgg tgtgtttttg tttgctcggg ctgcaaagat gaatttcccg ataaagagca
     2101 tgcccatcac tgtatggttt gtaatacaga gctctgtacc aattgcaata cgcattgcca
     2161 tgattgcggc catactgttt gcgaaacgca tagtactgtt tgttttgccg ataaagagat
     2221 tgtgtgtgat caatgtgtca aatcatgcag cgattgcgga cgtacgatgt gcagtgatca
     2281 tactttttta tgtcacgcta caaaggtacc gatttgtcac gaacaccggg tgatatgccc
     2341 caaatgcagg aagatttacg ccagagggtt tattgacaaa cagaaaaaaa atgatcgaaa
     2401 atgccccgag tgtttaacca ccttgtaaaa taaaaagccc gttgatatgc tcaacgggcc
     2461 ttttaaataa atttttatac cgtattattt caaacggaaa gtaaaaggat aggtaatcca
     2521 tacttttacc gggcgattac cgttaattgc cggtttaaat ttcatagaca tcaaagcgtt
     2581 aaccgccgat tctttgaaca tttcgtcacc ctgcaataca acgcattttg taaccgtacc
     2641 ggattcgtcg atcagcgcct tcacgatcac tttaccttcg agaccggcct ttttagccaa
     2701 ttccgggtaa accggctgta ctttcgccac gatcaccggt tgttgttcga cgggtacaaa
     2761 ttcatccata cccggttcac ctccaccgat atcgccgatc gttccggaaa tatccacctt
     2821 cacttcacca ccgtcgcttc cgaaactggt cggtgcagcg atatctgttt tgatttcttc
     2881 ttgtgtctgt atggtcatat ccggcttggc ttcggcatcg ggcaccacga tgggtatacc
     2941 ggccaccggt ttgatcactt cctgtacttc gacttttact ttcggcgcgg ccgtttgttc
     3001 ttgcagcggc ggagccgatt gtaattcgtt gatatccgta atacggacca tacggatttg
     3061 ttcttcttta aagactaccg tactgatgta gtacgtcata ataccggcta tatgcaatgt
     3121 aataccgata atcaaacctg tgctgacatt tttctgatat acctttttga gaacaaatga
     3181 accgtagttc tcccggccgt agccttcgat gacttcgatg ttggtcattg ggctttcgca
     3241 acctccttct ggtcttgctc gctaaaggga gcaaacgcaa atcgcggtga gagacgattt
     3301 gcttcggtta ggttattctt ttgattcagg tcaaacatgg caagattgag ttcgtccacg
     3361 atattgacca tgtgttcgta ttttgcccgt ttgtcaattt tgatgagaac gatcaattta
     3421 aatttatctt tgatcgtatc ccctttgccg ttggctatat ttttttcact gcgttcgatg
     3481 atgatttcac gcaattcctt catcgtagcc ttttgcggct tttcgttacc ctgattccaa
     3541 aaaatattat tatccaaatc tatacggatt tgcataatat tggactcggc tattttgacg
     3601 ttattttcag gcttatcatc ttcagggatg ctgatttcca tagtctgcgg cagacggaat
     3661 gctgtcgtaa gcatgaaaaa tgtcagcaga aggaacgcca catccaccat cggcgtcata
     3721 tccaaacgca caccggtgcg ggctttcttt ttcttcttgc ccttgccgtg ggatcggggt
     3781 tgtgcaccac ctacttcagc catgttagta ctccgctaaa attatttctc aagattggtg
     3841 ataaagttca ccgtcgtgat cttggatttt tgtaaaatgt caatgatttc gcttacgaca
     3901 ccgtaatcgg catctttgtc cgctttgagc accacacgca tagcaccgga agcgccacgg
     3961 atagccagat tttctaaacg cgctttggta ataaggttct ggagttcgtc cagtttaacc
     4021 gctttacctg cactttgtgc ataatctgca ccgaaaatct ttccgcgtac gacctgcgca
     4081 tccacatcca tccaaatgcg tgtgccggta ttttcttcgt tttgtgtgcc cttttttaga
     4141 ataatcggat ctttggcgac cgataaggta atcacattag ccgaaggcag cttcagctgt
     4201 gaatgtgaat ccggtacggt aaccgaaatt tcctccggtg gacgaaaggt cgtcgtaagc
     4261 atgaagaacg tcagcagaag aaacgcgacg tccaccatgg gcgtcatgtc aagctttacg
     4321 ccgacacgat gttttttatg ttttgccata gtatgttacc acgccttcaa attagttctt
     4381 atcgtcaccg tgagttgtgg tcagaatttc aactacattg tagctggctt cgtcaatcag
     4441 gtaagtatag gtgtccactt tattgacgaa gtagttatat gctacaatag ctacgatcgc
     4501 agcaccgaga ccaccggccg tattgataag tgcttcggag ataccgagcg agagggatac
     4561 ggcatccgga gcaccggatt gagcaagcgc tttgaatgcg cggatcatac cgagggtcgt
     4621 accaagcaat cccaccatgg tgga
//
LOCUS       DAOQNQ010000257         4615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_8054_length_4615_cov_4.864474, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000257 DAOQNQ010000000
VERSION     DAOQNQ010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8054_length_4615_cov_4.864474"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2327)
                     /locus_tag="PK754_12895"
     CDS             complement(<1..2327)
                     /locus_tag="PK754_12895"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNB10447.1"
                     /translation="MKKNTIVICITLILQGWPAFSQFTEVITGLPGLYGASAEFGDFD
                     GDGDLDILMGGVNRGIFRNDNGSFVNINADLEDYPYDVAWGDYDNDGDIDVLMNGAVY
                     QNNGGAFSLINTPVQDANEAKMQWIDYDGDGDLDISQIAHTGRIFKNNQGVFTQVEQI
                     QSFVGIGGGSWSDYNNDGYPDMFLTGTQGSTGTMTMYLYANQRGKLSQVSNQLKGFMH
                     GSMSWGDYDQDGDDDLLLTGGQIDTNLTYLYKNQGSSFLLANSTFPGYGYGDAAWGDI
                     DNDGDLDLALMGMGSTGKRVSIYRNNAGAFALMNVALPSLYFGGVVWGDYDNDGDLDL
                     LVSGSPSGFFPAIEVHLFRNDFATPNVSPSVPSDLTAWADSGSIMLSWSASDDDSTPS
                     QSLTYNVRIGKTQGGQEVLSAQSNLITGQRLVVGQGNAGHMLYKRIYPVTDGTYYWSV
                     QALDNNFQGSAFSSEQILVVNFKPDTVHNVRIQLDTSHIALSWSPNTEADLLRYRIYM
                     KMENSEWTEIDSVTRQDTTWQLNGPIIGKKYFFKVAAVDSSLQEGSSEQPVFATPQFF
                     EKTTSIPGLNYSVVAWGDYDNDGDLDLAISGYSNSSYSAIFRNDAGIFVNINATLASY
                     NTPGSLQWADFDRDGDLDLLSTGSNNGNNNAPSIFIYRNDASTFVSIATGIPNYEFAR
                     AVWGDYDNDGDLDIFVCGYGFSSNHSDLYRREGSSYVKTSYFFSGSDGPPYWIDVDND
                     GDLDIYLAKQNLLYRNEGNGFVLQHSFEYLDQARAS"
     gene            complement(2870..2947)
                     /locus_tag="PK754_12900"
     tRNA            complement(2870..2947)
                     /locus_tag="PK754_12900"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2910..2912),aa:Val,seq:cac)
     gene            complement(3099..4067)
                     /locus_tag="PK754_12905"
     CDS             complement(3099..4067)
                     /locus_tag="PK754_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNB10448.1"
                     /translation="MSVIAFAEQRGGAFKKSAFEAVAEAKRLADKLGTQAVAVVIGNN
                     VSGIAAELGKYGASKVYVAENAALENYSTEGYTQALNEAVKKENAAVVLLGATAMGKD
                     LAPRTAARLGAGIASDVTGLDVEGGKITITRPIMSGKAFQKLRITTATQVITIRPNVF
                     KATESGATAAVENLSMAIAPIRATVKNTVVESGKKVELTEADVVVSGGRGLKAPENWN
                     LVVELAEAFGGAHGASRAVVDAGWRSHEEQVGQTGKTVSPQLYIACGISGAIQHLAGM
                     SSSKYIVAINSDAEAPIFKIADYGIVGDAMEILPAMTAHVRKIKGK"
     gene            complement(4112..>4615)
                     /locus_tag="PK754_12910"
     CDS             complement(4112..>4615)
                     /locus_tag="PK754_12910"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein beta subunit/FixA
                     family protein"
                     /protein_id="HNB10449.1"
                     /translation="AILLKADAFDGDVAHALAEEIKAGAYDLVLFGKQAIDEDSSVMP
                     QMIAEKLNLPCVTVVTKLDVQAETKAITCEREIEGGKEIVELSYPAVIGTQKGINEPR
                     LPNLKGIMAAKKKTIDKKDPAKAPAMTEIVAIELPPARPAGKIVGKGADAVPELLRLL
                     HEEAKVI"
BASE COUNT         1227 a         1093 c          987 g         1308 t
ORIGIN      
        1 gatgcccttg cctggtctaa atattcaaaa ctgtgctgaa gaacaaaacc gtttccttca
       61 ttccgataca acaaattttg ttttgccaaa taaatgtcca agtcgccatc gttatcaacg
      121 tcaatccaat agggcggtcc atcagagcct gaaaaaaaat atgatgtctt cacataagaa
      181 gacccctctc tccgatacag atcgctatga ttgctagaaa agccatatcc acaaacaaaa
      241 atatccagat ccccatcgtt atcataatcg ccccatacgg ctctggcaaa ttcataattg
      301 ggaataccgg ttgctatcga cacaaaagtg gaagcgtcat tacgatatat aaatattgaa
      361 ggtgcgttgt tattgccgtt attgctgcct gtagatagta aatctagatc gccatctctg
      421 tcgaaatcag cccattgcaa acttccagga gtgttataac ttgcgagagt agcgttgata
      481 tttacaaaaa tcccggcatc attacggaaa atagcactat aacttgaatt ggaatatccg
      541 gaaatcgcaa ggtccaaatc gccgtcatta tcgtaatctc cccacgcaac gacggaatag
      601 ttcagccctg gaatcgaagt ggttttctca aaaaactgag gagttgcaaa aaccggctgt
      661 tcggaacttc cttcttgtaa agaactatct acggctgcaa ctttaaaaaa gtatttttta
      721 ccgattatcg gtccgttcaa ttgccacgtt gtatcctgtc tcgttaccga atctatctca
      781 gtccattctg aattttccat tttcatataa atgcgatacc tcaaaagatc ggcctcggtg
      841 ttgggcgacc aactcaaagc gatgtgactc gtatcaagtt gaatgcggac attgtgaaca
      901 gtatccggtt taaagtttac aacaagaatt tgttctgaac taaatgccga tccttgaaaa
      961 ttattgtcga gcgcctgaac gctccagtaa taagtgccat cagtaacagg ataaattcgt
     1021 ttataaagca tatggcctgc attgccttgt ccaacaacta atctttgccc ggtgatcaga
     1081 ttggattgtg cagaaagcac ctcttggccg ccttgagttt taccaattcg aacgttgtat
     1141 gtcaagcttt gtgaaggtgt agaatcatca tcagaagctg accaacttaa catgatactg
     1201 cctgaatccg cccaggctgt aaggtcggaa ggaacggaag gtgagacgtt aggcgtagca
     1261 aaatcgtttc ggaataggtg aacttctatc gcaggaaaga aaccactagg tgatcccgat
     1321 acaagtaaat ctaagtcacc atcattatcg tagtcacccc aaactactcc gccaaaatac
     1381 aagcttggga gcgcaacatt catgagtgca aaagctccag cattgtttct gtatatactt
     1441 acccttttac cggttgaccc cattcccatc aatgccagat cgagatctcc atcattgtct
     1501 atgtcgcccc aagcagcatc accatagcca tagcctggaa atgtggaatt ggccaaaaga
     1561 aaagagctgc cctgattctt gtataggtag gttaaatttg tatcgatctg tcctcctgtc
     1621 aaaagcaaat catcatcacc atcctgatca taatcacccc atgacatgga tccatgcata
     1681 aatcctttta gttggtttga tacctgactt agttttcccc tctgattggc gtagagatac
     1741 atagtcatgg tcccagtgct accttgagta ccagtcagaa acatatccgg ataaccatca
     1801 ttattgtaat cactccagct acctccaccg atcccaacga aactttgaat ttgttcgacc
     1861 tgagtaaata ctccttgatt atttttgaaa atacgtccgg tgtgagcaat ttgagaaata
     1921 tccaaatctc cgtctccgtc atagtcaatc cattgcattt tggcttcatt tgcatcttgc
     1981 acgggcgtat ttatcagtga aaacgcgccc ccgttatttt ggtaaactgc tccattcatt
     2041 aggacatcaa tatctccgtc attgtcataa tcaccccaag ctacgtcgta cggataatct
     2101 tctaaatcag cattaatgtt gacaaatgaa ccattgtcat tacgaaatat ccctcgatta
     2161 acaccgccca tcagaatatc caaatctccg tcgccgtcaa aatcgccaaa ttcggcagat
     2221 gcaccataca aaccaggcaa acccgttatg acttctgtaa actgactaaa tgcgggccaa
     2281 ccctgaagaa ttagggtaat gcaaattaca atagtgtttt ttttcatgat tcttttttgt
     2341 tattgtgatc aatgtatgat gacataatca ttaatacatc tctaaaagtt caataatatg
     2401 aaacgaacac ggcaaaaaac tatattttta acgaacatca aaagttggag gccaacggat
     2461 aggtcatttg gtatcggatt caagcagttg gtctgattga gtcacttaca agatcacaaa
     2521 cgatttcaaa ttcaatgatc gaacaattct cgtgattgat tatgggttat cgttttttta
     2581 tgatgcgaag ttttttgtta ataactttcc tgttaagatc aaaatttggc tctatctatt
     2641 agaaataggt aagtgtatag actatttatt taatttttaa tccagtattt taaattggat
     2701 ttttttctta aataggatat aataacaaat ccgaaaatca tttatctctc ccggtactat
     2761 ttcagggaag ttcttaccat attgcgcgtc cgatagtgta ttaaaatatc ccgcttcggg
     2821 cgggatggcc tttcgcatct aaaaaaatat atagaaaata ttttagaatt ggtgggcaat
     2881 actggattcg aaccagtgac ctttcgcgtg tgaagcgaac gctctgaacc aactgagcta
     2941 attgcccgaa tgtcgtacga agataaaata aaagccggtc aaagataggc ggctaaggct
     3001 tattttgcaa tgattttttc cgccggcagg tatacttcgt aaacaaaaaa gggctgatcg
     3061 cttatgcgtc agccctggca tgctaaaaat aaatcggact atttgccttt gatcttacgc
     3121 acgtgggcgg tcatggcagg caatatctcc atcgcatcgc ctacgatacc gtagtcggcg
     3181 attttgaata tcggcgcttc cgcatcgcta ttgatcgcca cgatgtattt ggaactcgac
     3241 ataccggcca aatgctggat cgcaccggaa atgccgcacg cgatatagag ttgcgggctg
     3301 accgttttac cggtctgacc gacctgctct tcgtgcgaac gccatccggc atctacgacc
     3361 gcacgtgatg cgccgtgggc gccgccgaaa gcttcggcca attctacgac taagttccag
     3421 ttttcaggcg ctttgaggcc gcgtccgccg gaaacgacga catcggcttc agtgagttcg
     3481 acttttttac cggattcgac gaccgtgttt tttaccgtag cacggatggg agctatcgcc
     3541 atcgacagat tttctactgc cgcggtcgcg ccggactcgg tcgctttaaa tacattgggg
     3601 cgaatcgtga tcacttgggt ggctgtcgta atgcgcagtt tttggaaggc tttgccggac
     3661 atgatcgggc gggtaatggt gattttaccg ccttcgacgt ccaaaccggt gacatcgctg
     3721 gcgataccgg cgcccagacg tgcagccgta cgcggcgcaa ggtctttgcc catcgccgtc
     3781 gcacccaaaa gtaccaccgc agcgttttct tttttgacgg cttcgttcag agcttgcgtg
     3841 taaccttcgg tcgaatagtt ttccaacgcc gcgttttctg cgacatagac tttggaagcg
     3901 ccgtatttgc cgagttcggc ggctatgccg gagacgttat ttccgatgac tacggcgacg
     3961 gcctgcgtac cgagtttatc ggccagacgt ttggcctcgg ccacggcttc gaaagccgat
     4021 tttttaaaag cgccgccgcg ctgttctgca aatgctataa cggacatata tactccatat
     4081 tattttttgg gttgatcgga tcgtaaaccg cttagatgac tttggcctct tcgtgcagca
     4141 ggcgaagcaa ctcgggaacc gcatcggcgc ctttgccgac gattttgccc gcaggacgtg
     4201 ccggcggcaa ttcgatcgct acgatttccg tcatcgccgg agctttagcc ggatcttttt
     4261 tatctatggt ttttttcttg gccgccatga tgcctttgag gttaggcaag cgcggttcat
     4321 tgattccttt ttgggtgccg atcacagccg gatacgaaag ttcgacgatc tctttgccgc
     4381 cttcgatttc acgttcgcag gtgatcgctt tggtctcggc ctggacatct aatttggtta
     4441 ccaccgtgac gcaggggaga ttcagttttt cagcgatcat ttgcggcatg acggagctgt
     4501 cttcgtcaat ggcttgtttg ccgaaaagta ccaagtcgta cgcgccggct ttgatttctt
     4561 ccgccagtgc atgggctaca tcgccgtcaa acgcatcggc tttgagcagg atcgc
//
LOCUS       DAOQNQ010000258         4595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_8115_length_4595_cov_5.742511, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000258 DAOQNQ010000000
VERSION     DAOQNQ010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8115_length_4595_cov_5.742511"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PK754_12915"
     CDS             <1..197
                     /locus_tag="PK754_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HNB10450.1"
                     /translation="LEAFMQMRGDLDQERRSMEKSWQRREKQIERMMVSMSRMNGDLE
                     GIMGTALPAIPALELPGDKS"
     gene            212..1504
                     /gene="hflX"
                     /locus_tag="PK754_12920"
     CDS             212..1504
                     /gene="hflX"
                     /locus_tag="PK754_12920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNB10451.1"
                     /translation="MNKTHDTTRKKERALLVGVAHDRQENRLVDDYLEELALLADTDG
                     VEVVASVKQQLRQIDPSTYIGKGKVHEIVLKVQAENIQVVIFDDDLSPGQTKNIEREA
                     KCKIIDRSALILDIFAKRARTRESKAQVELAQLEYLLPRLSKMWTHLERQGGGGVFTK
                     GPGETQLETDRRLVGQRIAVLKEELKKIERQRETQRAGRGEVFRVALVGYTNVGKSTL
                     LNALSQADVYVEDRLFATLDATTRKVFLDNDHSCLITDTVGFIRKLPHHLVASFRSTL
                     EEVREADILLHVVDLSSPIFMEQMEAVEKVLRELNALKDARIVVFNKVDRVSDEALIT
                     SMRHQFPNSVFISAGRRIGLDRLKQAIKDEIDHAFTLDKVSFDSGDGKFYATVHHLAE
                     VLEETYTDSKTTLHFRALKVNAERIKKLQSERADWDKV"
     gene            complement(1518..2777)
                     /locus_tag="PK754_12925"
     CDS             complement(1518..2777)
                     /locus_tag="PK754_12925"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNB10452.1"
                     /translation="MDLLHKLKQVSEEAERLLAIVEIGKLLNSTIHLDQLLEIILDTA
                     LQNLQAERGTLYLIDLEKKELWSKVLKGEETIRLPIGKGIAGTVAATGETIFLTDAYD
                     DPRFNPEFDKKSGFRTSSMLCTPMKNREGRMIGVFQILNKREGEFEKSDIEFLDTLSL
                     DACIAIENARLHEADLVKQRILKELEVAATIQRMILPKEITSIPGLDLAGLNVPSRQV
                     GGDYYDAVPLPDGRVALVMADVSGKSVPGALLVSTLQASLRAYLESPFELAPLVTKLN
                     RVILKNSTDDKFITFFIGIVDTQKNTLEYLNAGHNPPLLMTKGQLIKLTKGGIPLGMI
                     GFDDYAAQTLNLHSGDTLVLFTDGITEAVSPNDDMYDDARLENLVQNVAHSSAEDLKK
                     IIYNDVMAFVNGAEQADDITLLIAKYQ"
     gene            3082..3549
                     /gene="rimP"
                     /locus_tag="PK754_12930"
     CDS             3082..3549
                     /gene="rimP"
                     /locus_tag="PK754_12930"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNB10453.1"
                     /translation="MNNLQTRIRNIVSKIIENTEFKIVDIDLKSTSGRYMLRVFLDSE
                     KGIIIDRCREFSREIETVLDAENFLGENYLLEVSSPGLERPISQDWQFRKNMGRTIIM
                     TYLSDQGDKTLEGTVVGVGDNNVTLRVQLAKKQTSDITIELSQIKQAKIKLKW"
     gene            3614..>4595
                     /gene="nusA"
                     /locus_tag="PK754_12935"
     CDS             3614..>4595
                     /gene="nusA"
                     /locus_tag="PK754_12935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNB10454.1"
                     /translation="MEHGGMIVDAFTQIAREKNIEKDELNLVIEEIFKMMIKKKYGST
                     DNFDVIVNLDKPAIEIYQNVVVADPVTNPVAEISIEDAQKMDPDMKVGDDYVHIIDPA
                     SFGRRLIISARQTLNQKIRDIERKLIMDEFKNRIGEIVVGEIRQINKNEIYISFERTE
                     LIMPKMEQISNERYKRSDTVRAVIKDVVDDPRGPRIVVSRAQPEFLQRLFENEVPEIY
                     DGIIEIKAIARDPGERTKIAVFSNDKRIDAVGACVGMKGVRIQAIVKELNNEKIDIVS
                     WSPDAEIFISRALSPAKPLRVEVDTTDKKALAVINDDEMSIAVGRFGQNIN"
BASE COUNT         1389 a          886 c         1067 g         1253 t
ORIGIN      
        1 tacttgaggc ttttatgcaa atgcgtggtg atttggatca ggagcgccgc agtatggaaa
       61 agagttggca acgccgagaa aaacaaattg aacgcatgat ggtgagcatg agccgaatga
      121 acggcgactt agaaggcatc atgggcacgg ctttaccggc tattccggcg ttggaattac
      181 ccggtgataa atcataaaat ttcaggagaa tatgaataaa acacacgata cgacacgaaa
      241 aaaagaacgc gcattacttg taggcgttgc acatgatcgc caggaaaaca ggttagtgga
      301 tgactatctt gaagaacttg ctttgttagc ggataccgac ggtgtggaag tggtggcatc
      361 cgtgaaacag cagcttcggc aaattgatcc atccacatat atcggtaaag ggaaggtgca
      421 cgagatcgtg ctcaaagtgc aggccgagaa tattcaggta gttatatttg atgatgatct
      481 ttctccgggc caaacaaaaa atatcgagcg tgaagcgaaa tgtaaaatca tagatcggag
      541 cgcgttgatt cttgatattt ttgctaagcg tgcacgcacg cgcgaatcga aagcccaagt
      601 tgaattggca caactcgaat atctgcttcc aagactttct aaaatgtgga ctcacttgga
      661 acggcaaggc ggcggcgggg tttttaccaa aggtcccggc gaaacgcagc tcgagacgga
      721 tcggcgtttg gtcggacaac gtattgccgt tttaaaggaa gaacttaaaa aaatcgaacg
      781 acagcgggaa acccaacggg cgggacgcgg tgaggttttt cgtgtagctt tggtcggata
      841 taccaatgtc ggcaaatcta cattactcaa cgcactttca caggccgatg tgtacgtgga
      901 ggacaggctt tttgctacgc ttgatgcgac gacgcgcaaa gtgtttttag ataacgatca
      961 ttcctgtttg ataacagata cggtcggttt tatcagaaag ctgccgcatc acttggtcgc
     1021 ttcatttcgc tccacgctcg aagaagtacg cgaagcagat attttattgc atgtcgtgga
     1081 tttgagcagt cctattttta tggaacaaat ggaggcggtc gaaaaagtat tgcgtgaatt
     1141 aaatgcactc aaagatgcac gcatcgtcgt atttaataaa gtggatcggg tgagcgatga
     1201 agcgctgatc acgagtatgc gtcatcagtt tcccaatagc gtgttcatat cggccggtcg
     1261 ccgtatcggg ttggatagac tgaaacaagc tatcaaggac gaaatagatc atgcgttcac
     1321 gttggataaa gtttcgtttg atagcggtga tggtaagttt tatgcgaccg tacaccatct
     1381 agcggaagtt ttggaagaga cgtacacgga ttctaaaacg acacttcatt tccgtgcatt
     1441 aaaggtcaat gccgaacgta taaaaaaact acaatccgaa agagcagatt gggataaagt
     1501 gtgagaacga gctgagttta ttggtatttt gctattagta atgtgatgtc atcggcttgt
     1561 tcggcaccgt tcacaaaagc cattacgtcg ttatagatga tttttttaag atcttccgcg
     1621 gaagaatgcg cgacattttg tactaaattt tctaaccgtg catcgtcata catatcgtca
     1681 ttaggggaaa cagcttcagt aatcccgtcg gtgaataaga ccaacgtatc acctgaatga
     1741 agatttaacg tttgcgcggc atagtcatca aagccgatca tacctaacgg gatacctcct
     1801 ttagtcaact tgatcaattg acctttggtc atcaaaagcg gagggttgtg tcctgcgttg
     1861 aggtattcga gcgtgttttt ttgtgtatct actatgccga tgaaaaaagt tataaatttg
     1921 tcatcggtcg aatttttgag tatgacacga ttgagtttgg ttacgagcgg tgcaagttca
     1981 aaagggcttt ccaaataggc gcgtaacgaa gcttgtagag tggatacaag caatgcaccg
     2041 ggtacgcttt taccggatac atcggccatg accaaagcca cccgtccgtc ggggagggga
     2101 acggcatcat agtaatctcc tccgacctgt cgactgggga cattcaatcc ggccagatca
     2161 agaccgggaa tggaggtgat ttcttttggt aatatcattc tctgaatagt ggctgcaact
     2221 tcaagctcct ttagaatgcg ctgttttacg agatcggctt catgcaaccg cgcattttcg
     2281 atcgcgatac atgcgtcgag tgataacgtg tccaaaaatt cgatatccga tttttcaaat
     2341 tcaccttcac gtttattgag aatttggaaa acaccgatca ttcggccttc tctgtttttc
     2401 atcggtgtgc ataacatact gctcgtgcga aatcctgatt ttttatcaaa ttcagggtta
     2461 aatcggggat cgtcataggc atcggtcagg aatatcgttt ctcctgtagc tgcaacggtt
     2521 cccgcaatac ctttgccgat aggaagccgt atggtctctt cgcctttgag tactttggac
     2581 cacaattcct ttttttcaag atcaatcaga tacaatgttc cgcgttccgc ctgaagattt
     2641 tgtaatgcgg tatccaatat aatctcaagc aactggtcca gatgaattgt cgaattgagt
     2701 aattttccga tttcgacgat agccagtaag cgctcggctt cttccgaaac ttgtttgagt
     2761 ttgtgaagca aatccataat gtaaacctat aagtgtattc caaaaataag caataattag
     2821 tttaattcaa caaaattaac atcgagtatg atacagagcg caagaaagta tgaaagatag
     2881 ttgttttgaa ttttttttca tatgaactca agtcagatta aatttacaaa tccttaaaaa
     2941 acaacatcaa tagtctcaca aagttgaatc aaaaaatact tgcattacgt aaaaagaagc
     3001 tttatattta cggacagctt ctttacctat tacggagtgg gttcgcaaga cccacttttt
     3061 tgttttcata aagttataaa agtgaataat ttacaaacgc gtatacgaaa catagtcagt
     3121 aaaattattg aaaatactga atttaaaatt gtggatattg atttgaaatc cacatccggt
     3181 cgttatatgt tgcgcgtatt tctcgatagt gaaaaaggga taataataga ccgctgccgt
     3241 gaatttagcc gtgaaatcga gacagttctt gatgcggaaa attttttagg tgaaaattat
     3301 cttctcgaag tttcgtctcc cggtttggag cgtccgatct cacaagattg gcaatttcgc
     3361 aaaaacatgg gcagaacaat aataatgaca tatttatcgg accaaggcga taaaacattg
     3421 gaaggaacag tggttggcgt gggagataat aatgttacgc ttcgtgtcca gttagcaaaa
     3481 aaacaaactt cagatatcac tattgaactt agccaaatca aacaggcaaa gatcaaactg
     3541 aagtggtagt tgtaaaaaga ataaattaaa aaacaaatta atttaatata gtgaaccatt
     3601 tatcggaggc agcatggaac acggtggcat gatagtcgat gcgtttacgc agattgcccg
     3661 tgaaaaaaac attgagaagg atgaactcaa tttggtcatc gaggaaattt ttaaaatgat
     3721 gatcaaaaaa aagtacggct caacggacaa ctttgatgtg atcgttaact tagacaaacc
     3781 ggcaattgaa atttaccaaa atgttgtcgt tgccgatccg gtaacaaatc ctgtggccga
     3841 gatttccatt gaagatgctc aaaaaatgga tccggatatg aaagtcggcg acgattatgt
     3901 gcatatcatt gatccggcaa gtttcggtcg tcgtcttatt atcagtgcac gtcagacact
     3961 caatcagaaa atacgagaca tcgagcgcaa actgatcatg gacgaattta aaaaccgtat
     4021 cggcgaaatc gttgtcggtg aaatccgcca gatcaacaaa aatgaaattt atatcagttt
     4081 tgagcgcacc gaacttatca tgcccaaaat ggaacaaatt tccaatgaac gttacaaacg
     4141 cagtgatacg gtgcgtgccg ttatcaaaga tgtcgtggat gatccgcgtg ggccgcgtat
     4201 cgtggtatct cgtgcgcaac ccgaatttct gcaacgtttg tttgaaaacg aagtacctga
     4261 aatatatgat ggtattatcg aaatcaaagc tattgcccgt gatccgggtg agcgtacgaa
     4321 aatcgccgta ttttcaaacg ataagcgtat tgacgcggtg ggcgcgtgtg tcggtatgaa
     4381 aggcgtacgt attcaggcga ttgtcaaaga acttaataac gaaaaaattg atattgtcag
     4441 ttggagtcct gacgcggaaa tttttatttc acgcgcgttg agtccggcca aacccttgcg
     4501 tgtcgaagtg gataccaccg ataaaaaagc cttggctgtt atcaatgacg atgagatgtc
     4561 catcgcggtc ggccgcttcg gtcaaaatat aaact
//
LOCUS       DAOQNQ010000259         4563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_8221_length_4563_cov_4.368456, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000259 DAOQNQ010000000
VERSION     DAOQNQ010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8221_length_4563_cov_4.368456"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PK754_12940"
     CDS             complement(<1..640)
                     /locus_tag="PK754_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009057036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HNB10455.1"
                     /translation="MSKIFDRVNLPKDIKSLSSDELVQLAGELRAYVIDEISRIGGHL
                     APSLGVVEISVALHHVFDTPTDKVIWDVGHQAYVHKLLTGRKDQFHTIRQTDGISGFC
                     KIHESEYDAFGAGHASTSISAAVGMAIARDQLKKNNKVVAVIGDGSLTGGMAFEAMNN
                     AGSLKTDMIVVLNDNMMSISSNVGALSNYLTDLISAPLYNKVKTEVWNALGHL"
     gene            968..2209
                     /gene="glgC"
                     /locus_tag="PK754_12945"
     CDS             968..2209
                     /gene="glgC"
                     /locus_tag="PK754_12945"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNB10456.1"
                     /translation="MTPISSSLFRKTLAIILAGGQGERLYPLTKDRSKPAVPFGGKYR
                     IIDFALSNAINSGFRKIYLITQYKSQSLDTHIREGWSFLNGELGEYIYCIPPQFRTAN
                     HWYQGTADAVFQNLHVLQDDKPEYVIILSGDHIYKMDYLKMLQYHIAKQADLTIAGIE
                     FQRDQAHQFGVMVVNEDNDIIEFQEKPKEPKPLPNKPDFCYVNMGIYIFSADAMAHVL
                     VDDAKLKDSAHDFGKNVIPSMLGKYKVTAYNFEDENKAGQPYWRDIGTLDSYFDTSMD
                     LISVSPTFNLYDIDWPIRTYQGQYPPAKNVFSEPQEGGRMGVALDSIVSGGVIISGGR
                     VTRSLLSPNVRIHSYSSVDESILFNNVSVGRNSKIKRAIIDKNVVIPENSEIGYNLEE
                     DKKRFFVTPSGIVVISKNQRI"
     gene            complement(2210..2683)
                     /gene="ispF"
                     /locus_tag="PK754_12950"
     CDS             complement(2210..2683)
                     /gene="ispF"
                     /locus_tag="PK754_12950"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNB10457.1"
                     /translation="MRIGQGYDVHRLAEGRKMILGGVEIPADKGPLGHSDADVLLHAI
                     CDALLGAIGLGDIGKHFPDTDQAYKNISSLILLEKVYALITERNYRVGNVDATLVLQK
                     PKIAPHVPAMKKNIAAVLHVEEERVSIKATTSEGLGFVGVGDGVVAYAVALVFKS"
     gene            complement(2728..>4563)
                     /locus_tag="PK754_12955"
     CDS             complement(2728..>4563)
                     /locus_tag="PK754_12955"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNB10458.1"
                     /translation="DYNAKSALNGISENTYREEHFHSAATTTDAELRHFADALYEVKK
                     IIGIEGFSVGSNNWVVSGARSVSGKPLLANDPHLGHAMPPTWYEMHLRGGRLDVAGFT
                     LPGVPLIVLGNNNRIAWGFTNVMMDDADFYREQIRDSMYYFNGAWRKLGWAEEKIVIY
                     DSTDVVFRIPMTHRGPIVQGFYANVSGSEAISVRWSGYEATREVEAMLGMNIAQDWTG
                     FRKAASEFKIPAQNIVYADVDGHIAYQCVGNIPLRRNGNGISILDGSVLQNDWAGYLP
                     FESNPYRLDPPEGFIASANNKTAGDWFPYFISNYWEHPSRIKRIHDFMNGQEKFSPAD
                     FQKFQNDFYSYHALEVIPYILEACSKDSNFTKQDLEHVSNYRYNESYLFLKHWDGRMH
                     ADSRGAAIFNVFFRRLTRNLYSDEMGDDLYRDFVKLTNIPTRVTTQLLQLPKSAWWDN
                     VRTREIEDRDAILRITMTETVDELTADLGSEPGGWAWGKIHHVEFEHFVGRQKPLNYL
                     FNVGPFAVGGNTTTINNTEFNYSDSTYKAVIGASMRRIVDLADIQHPKTVLTVGQSGQ
                     PFSLHYDDQVRMWLDGQYKIINLDWGALRNNPSHHRLILMPPNAK"
BASE COUNT         1261 a         1145 c          953 g         1204 t
ORIGIN      
        1 gcaaatgacc gagcgcattc cacacttccg tttttacttt attatacaac ggtgctgaaa
       61 taagatccgt aagataattg gataaagctc cgacattgga agaaatggac atcatgttat
      121 cattgagtac gacgatcatg tcggttttaa gcgaacccgc attattcatg gcttcaaaag
      181 ccattccgcc ggtgagcgaa ccgtcaccga tcacagctac gactttgttg ttttttttca
      241 attgatcgcg ggcgatcgcc ataccgacgg ccgccgaaat cgaagtgctg gcatggcctg
      301 caccgaaagc gtcgtattca ctttcatgaa ttttacaaaa tccgctgatg ccgtccgttt
      361 gtcgtatcgt gtgaaattga tctttacgtc ctgtgagaag tttatgcaca taggcctgat
      421 gccccacatc ccaaataact ttatccgtcg gcgtatcaaa tacatgatgc agtgcgaccg
      481 atatttctac gacgccgaga ctcggggcga gatggccgcc gatacgtgaa atttcatcaa
      541 tcacataggc gcgcagttcg cctgcaagtt gcactaattc atcggacgac aacgatttaa
      601 tatctttagg aagattcact cgatcaaaga tcttggacat gccattcgct ccaaacagtg
      661 gtaaacaaag ccttagaagc ttttatctct aaagctaaca cataacgggc gcaaattacg
      721 tatagcgcta taggaatgca acgaaaaact aattacagaa gcagcggccc attcagtact
      781 caaatcgctt tttctatgga aatccagtga taatagctgt gcgttagtat gacttaaaga
      841 aagactgttt ttgtgatggc gcacgcttag aaaaaatatt tggacttcgc ccgcgacttt
      901 aatacattta aaaactaatc tatctgttct caacggaata catctaacta ctgaacggga
      961 gcatggcatg acgccgattt catcatcgct tttccgcaag accctcgcta ttattttggc
     1021 cggcggtcaa ggcgaacgtc tatatcctct caccaaagat cgctccaaac ccgcagtccc
     1081 gtttggcggg aaataccgca tcattgattt tgccctcagt aacgcgatca attccggttt
     1141 tcgtaaaata tatctgatta cacaatacaa atcgcaatcg ctcgatacgc atatccgcga
     1201 aggctggagt tttctcaacg gggaactcgg tgaatatatt tactgcattc cgccgcaatt
     1261 tcgtacggcc aatcactggt atcagggcac ggccgatgcc gtatttcaaa atcttcatgt
     1321 gctgcaagat gataaaccgg aatatgtgat cattctctcc ggtgatcaca tttacaagat
     1381 ggattatctc aaaatgcttc agtaccacat tgccaaacaa gccgatctga ccattgccgg
     1441 gatcgaattt caacgcgatc aagcccatca gtttggcgtg atggtggtca atgaggacaa
     1501 tgatatcatc gaattccagg aaaaacccaa agaacccaaa ccgcttccga acaagcctga
     1561 tttctgttac gtcaatatgg gtatttatat ttttagcgcc gatgcgatgg cgcacgtttt
     1621 ggtagatgat gccaaactaa aagattcggc ccacgacttc ggtaaaaatg tcattccttc
     1681 catgctcggt aaatacaaag tgacagccta taattttgaa gacgagaata aggccggtca
     1741 accctattgg cgcgacatcg gtacactgga tagttatttt gatacaagca tggacttgat
     1801 ttccgtttca ccgacattca acctgtacga tatagactgg ccgatacgca cataccaggg
     1861 acaatacccg ccggctaaaa atgtattttc cgaaccgcaa gaaggcggac gtatgggcgt
     1921 ggcacttgat tcgatcgttt cgggcggcgt gattatttcc ggcggtcgtg taacacgatc
     1981 cttgctttca cccaatgtcc gtatccacag ttattcgagc gtcgacgaat ccattctttt
     2041 caataatgtc agcgtcggac gcaattcgaa aatcaaacgt gcgatcatag acaaaaatgt
     2101 cgtcatcccg gagaattcgg aaatcggcta caatcttgaa gaggataaaa aacgattttt
     2161 tgtgacgcca tcaggcatcg tcgtaatatc aaaaaatcaa cgaatctgat caggatttaa
     2221 aaaccaacgc gacggcatac gctacaactc cgtcacctac accgacaaac ccaagtcctt
     2281 ccgaggtcgt ggctttgatt gacacccgtt cctcttcgac atgcaaaacc gcagctatgt
     2341 tttttttcat agccggcaca tgcggcgcga ttttaggttt ctgtaaaact aatgtcgcat
     2401 ccacattccc cacacgataa tttcgctcgg tgatcaacgc gtaaactttt tcaagtaaaa
     2461 taaggctgga aatatttttg taggcttgat cggtgtccgg aaaatgttta ccgatgtcgc
     2521 cgaggccgat agcccccaaa agcgcatcgc agatcgcatg cagcagtaca tccgcatccg
     2581 agtgccccaa agggcctttg tccgcaggaa tttccactcc gcccaatatc atcttacggc
     2641 cttccgcaag ccgatgcaca tcgtaacctt gtcctatacg aatatccata acgcctttat
     2701 gtttacagtg aaattatttt ataaaattta ttttgcattc ggaggcatca atatcagccg
     2761 gtgatgcgac gggttattgc gcagagcgcc ccaatccaaa ttgattatct tatactgccc
     2821 gtcaagccac atacgaacct gatcatcgta gtggagacta aacggttggc cgctttgtcc
     2881 gacggtcaag accgttttcg gatgctgtat atcggcaaga tctacgatgc ggcgcatact
     2941 cgcaccgatc acggctttgt aggtggagtc actgtaatta aattcggtat tattgatagt
     3001 cgtagtatta ccgccgaccg caaacggacc gacattaaaa agataattca atggcttctg
     3061 tcgccctacg aaatgttcaa attcaacatg atgaatttta ccccacgccc atccgccggg
     3121 ctcgctgccc aaatcggccg ttaattcatc caccgtttcc gtcatcgtaa tacgaagtat
     3181 tgcatcgcgg tcttctattt cgcgggttcg tacattatcc caccatgctg actttggaag
     3241 ttgcaggagt tgcgttgtca cccgtgttgg aatattcgtc aattttacaa aatcgcgata
     3301 cagatcatcc cccatttcat ccgaatagag gttgcgcgtc aatcgcctga aaaaaacatt
     3361 aaaaatagcc gcaccccggc tgtccgcatg catccgacca tcccaatgct tcagaaataa
     3421 atacgattcg ttatagcgat aattggatac atgctcaaga tcttgttttg taaaattcga
     3481 atccttactg cacgcttcaa ggatatacgg aatcacttct aatgcgtgat acgaataaaa
     3541 gtcattttga aatttttgga aatcggcagg actaaatttt tcctgcccat tcataaaatc
     3601 atgaatgcgt ttgatgcgcg acggatgctc ccaataattg gaaatgaaat acgggaacca
     3661 atcgccggcc gttttgttat tagcactggc aataaaacct tcgggcgggt caagtcggta
     3721 tggattcgat tcaaaaggta aataaccggc ccaatcattc tgtaatacgc ttccgtccaa
     3781 aatggaaatg ccgttaccat tgcgccgcaa tggaatgttc cccacacatt gatacgcaat
     3841 atgtccgtcc acgtctgcat acacgatatt ttgcgccggt attttaaatt cggaagcggc
     3901 tttgcgaaaa ccggtccaat cctgcgcgat attcattccc agcatcgcct ccacttcgcg
     3961 cgtggcttca tatccgctcc atcgcacgga aatcgcttcg ctacccgaaa cattggcata
     4021 aaacccttgt acgatcggcc cacggtgtgt catcggaatg cggaatacaa catcggtcga
     4081 atcataaatc acaatttttt cttccgccca gccgagctta cgccaagcgc cgttgaaata
     4141 atacatgctg tcacggatct gctcacggta aaagtctgca tcatccatca tcacattggt
     4201 aaaaccccag gctatccgat tgttattccc caaaacgatg agcggcacac cgggtagcgt
     4261 gaaaccggct acatccaatc ttcctccgcg cagatgcatc tcataccaag tcggcggcat
     4321 cgcatgcccc aggtgcggat cattggccag taacggttta ccggaaaccg accgtgcgcc
     4381 ggatacaacc caattattgc ttcctacact aaatccttcg atgccgatga tctttttcac
     4441 ttcatatagg gcatcggcaa aatgacgcag ttccgcatca gtagttgtag ccgcggagtg
     4501 aaaatgttct tcacgatatg tgttttctga aatgccattc aaagcggatt tagcattgta
     4561 atc
//
LOCUS       DAOQNQ010000260         4558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_8245_length_4558_cov_5.639796, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000260 DAOQNQ010000000
VERSION     DAOQNQ010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8245_length_4558_cov_5.639796"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PK754_12960"
     CDS             complement(<1..211)
                     /locus_tag="PK754_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HNB10459.1"
                     /translation="MQHYVHGWYSERLGKQMNFQHFGHGGAPVVVFPTTLGNLYEFSD
                     RRMIDPLAEKIENGLIQILCVDSINN"
     gene            complement(255..1007)
                     /locus_tag="PK754_12965"
     CDS             complement(255..1007)
                     /locus_tag="PK754_12965"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNB10460.1"
                     /translation="MRFAIVFLFFYISGIAHVTAGDPLRTLPNKAFPVGEKLKFSIGW
                     EFISAGTATLEVEQLTAHRDRPCYLVSATTVSNAFFSTLYPVKDRLESYIDQNGLYPL
                     KYVKNTSEGGYKRNFTTEFYHEQRKATIDDRDSGYKEIAIPEMVQDIISAFYYLRNQS
                     LEIGKTLSLATYDNGKINQATVKVVRKETISVDAGEFHCILVQTPIGPFNNKSDLNIW
                     LTDDDRKIPVLMKSKIAIGSVRAELVEMSGTF"
     gene            complement(1007..1846)
                     /locus_tag="PK754_12970"
     CDS             complement(1007..1846)
                     /locus_tag="PK754_12970"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10461.1"
                     /translation="MSKKKEKKKSEKKQGKKKPVKPVSVKKSTGKKAGSKPASKKAKP
                     TKKSVAAKSPSKNTAPSVDKKQASAKSTKKQPSRPPIPRTPADNVRRPFNIIESYMGE
                     YRFNDMFAILFVCTGNMCRSPLAEGIFKRKLAIECPESIKHKIWVESCGIYAYDGNKP
                     SENAIKVGHQNGVDISAIRSKPINRVLVEQSDLIFALSIDHLNYIQENFPTARHKTYL
                     MKVFGKDRGVTISDSIPDPMGFSMDFYLKTYNEIHVTIDEIFQKVVAMAEQKLNPKSS
                     ESM"
     gene            complement(1862..2947)
                     /locus_tag="PK754_12975"
     CDS             complement(1862..2947)
                     /locus_tag="PK754_12975"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNB10462.1"
                     /translation="MNYILREAWDVLKNPGIIPKLPNMMRASASYALNKWTRKNIVWG
                     YPFVMMVEPTNICNLRCPLCITGSKLMTRDDGMMTLERFKQLMDEMGPYLMHLTLWSQ
                     GEPFVNRDFVKMIRYAHDKHIKTVTSTNGHFLSENAEAIVESGLDTLIIAVDGASQDT
                     YEKYRVNGKFEKVFNGMKAIAAAKTKLKRKTPAIELQFIVMKHNEHELETIRQMAHEC
                     HVQVLSLKTAQVYTAEQALEFLPNNEKYRRYEIGNDGTFAAKIGSINFCRWVLMCPVI
                     NWDGTVSPCCFDKNADYPLGNVFDPGGMKTIWKNKAYHDFRRRIFTQRSAIPICSNCS
                     EGLEVEVYEKELIPSRPASAVALDHVA"
     assembly_gap    3219..3318
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3319..4547)
                     /locus_tag="PK754_12980"
     CDS             complement(<3319..4547)
                     /locus_tag="PK754_12980"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNB10463.1"
                     /translation="MNILVLNSGSSSVKFQVINTDLTQIRDNADTRLASGIIERIGSE
                     SIISFKAEGQPTLKQTAPMRDHKMAVDKIVKWIIGKDTKINGINGLSDIHAVGHRVVH
                     GGEKFTRSILLNEQIIQEIEDCIELAPLHNPANLKGIYAVTEIFGAGIPQVAVFDTAF
                     HHTIPEEAYLYAIPYQFYRRYKVRRYGFHGTSHRYVAYRYRTLTGQAKEQVHIMTLHL
                     GNGASICAIRGGKSVDTSMGLTPLEGLVMGTRSGDIDPAVLEFLSHKEGYSLQEIESV
                     LNKQSGLLGISGLTNDMRDIIAEAKENDDRRAKLAIEIYCYRIRKYIGAYCAAVGKPE
                     ALVFAGGIGENSAYIRESICKGMEFFGLTLDPHLNAATVGGKEGVISSPDARLKTWVI
                     PTNEELLIARDTVRCVE"
BASE COUNT         1078 a          987 c         1078 g         1315 t
ORIGIN      
        1 cgttattgat cgaatccacg cataaaattt ggatcagtcc gttttcgatt ttttcggcga
       61 gcggatcgat catacgacga tcggaaaatt cgtacagatt gccgagtgtg gtgggaaata
      121 ccacgaccgg cgcgccgccg tggccaaaat gctgaaagtt catttgtttg ccgaggcgtt
      181 cggaatacca tccgtgaacg taatgctgca tggttttctc ccgttagcgt accaatgaca
      241 ggtttgatgt gaacttaaaa agtaccgctc atttcgacga gttcagcccg taccgatccg
      301 atggcaattt tggatttcat caaaaccggt attttgcgat catcgtcggt aagccaaata
      361 ttcagatcgg atttattatt aaaaggcccg atcggcgttt gtacaagaat acaatgaaat
      421 tcaccggcat ctacggaaat ggtttcttta cgaaccacct tcaccgtggc ctgatttatt
      481 ttgccgttgt cgtacgtcgc gagcgatagc gtttttccga tttccagcga ttgattgcgc
      541 aaataataaa atgcagaaat gatatcctgt accatttcag gaattgcaat ttctttgtat
      601 ccggagtcgc gatcatctat cgtggctttg cgttgttcgt gataaaactc ggtagtaaaa
      661 ttacgtttat agccgccttc ggaagtattt ttcacatatt tcaggggata caatccgttc
      721 tgatcaatgt acgactccaa acgatctttg accggataca gggtagaaaa aaaagcattg
      781 gatacggtcg tcgcggaaac aagataacac gggcgatcgc ggtgtgcggt gagttgctct
      841 acctccaatg tcgcggttcc cgcgctgatg aattcccagc cgatggaaaa tttgagtttt
      901 tcccctacgg gaaacgcctt attgggcaac gttcgcaggg ggtcgccggc cgtgacatgt
      961 gcgataccgc ttatataaaa gaacaaaaaa acgatggcaa aacgcattac atgctttcgg
     1021 atgatttggg gttcagcttt tgttcggcca tagcgacgac tttttgaaaa atctcatcta
     1081 tggtaacatg gatttcatta taggtcttca ggtaaaagtc catactaaaa cccatcggat
     1141 cgggaatcga atcggaaatc gtgacgccgc gatctttgcc gaatactttc atcaaatatg
     1201 ttttatgacg cgcggtcggg aaattttcct gaatataatt caaatgatca atggacaacg
     1261 caaaaataag atcggattgc tcgacgagca cgcggttgat cggtttggat cgaattgcag
     1321 aaatatcaac gccgttttga tggccgactt tgatggcgtt ttccgaaggc ttattcccgt
     1381 cgtacgcgta tatgccgcac gattctaccc agattttgtg tttgatggat tcgggacact
     1441 caatggccaa tttgcgttta aagatacctt cggctaaagg actgcggcac atattaccgg
     1501 tacaaacaaa aagtattgca aacatgtcgt taaaacgata ttcacccata taactctcaa
     1561 ttatgttaaa aggtcgccgc acgttatctg ccggtgtacg cgggatcgga gggcgggacg
     1621 gttgtttttt ggttgactta gcggacgcct gttttttatc aacagagggg gctgtatttt
     1681 tagacgggga tttggccgcg acactttttt tggtcggttt tgcttttttg gatgcaggtt
     1741 tagaaccggc ttttttgccg gttgattttt ttacggaaac cggtttgacc ggtttctttt
     1801 tgccctgctt tttttcggat ttcttttttt cctttttctt gctcatggcg gccttttatg
     1861 gttaagcgac gtgatccagc gctaccgcag atgccggtcg tgacgggatc aattcttttt
     1921 cataaacttc gacttcgagt ccttcggaac agttgctgca aatcggtata gcgctgcgct
     1981 gtgtaaaaat gcggcgacgg aaatcatggt acgctttatt tttccaaatg gttttcattc
     2041 cgccgggatc aaatacattg cccaaagggt aatccgcgtt cttatcaaaa cagcatggcg
     2101 atacggtacc gtcccaattg atcacagggc acatcaatac ccatcggcaa aaattgatgg
     2161 atccgatttt tgcggcgaac gttccgtcgt taccgatttc ataacgccgg tatttttcgt
     2221 tgtttggtaa aaactccaga gcttgttccg cagtatagac ttgcgcggtt ttgagcgata
     2281 atacctgtac atggcattcg tgcgccattt gacgaatggt ttccagttca tgttcgttat
     2341 gtttcatgac gatgaattga agttcgatcg ccggagtttt tcgttttaat ttggtcttag
     2401 cggcagcaat ggctttcata ccgttgaata ctttttcaaa ttttccgttc acgcggtatt
     2461 tttcgtacgt atcctgtgat gcgccgtcca cggcaatgat cagcgtgtct agaccggatt
     2521 cgacgatcgc ttcggcgttt tcactgagga aatgaccgtt ggtgctggta accgttttga
     2581 tatgtttgtc atgcgcataa cggatcattt ttacaaaatc acgattgacg aaaggttcgc
     2641 cttggctcca tagtgtgaga tgcatcagat aggggcccat ttcgtccatc aattgtttaa
     2701 atcgttccag tgtcatcatg ccatcgtcgc gggtcatcag tttgctcccc gtgatgcata
     2761 gcgggcaacg gaggttgcag atattggtag gctccaccat catgacaaaa ggataacccc
     2821 acacgatatt ttttcgtgtc catttattaa gggcgtacga tgcggatgcc cgcatcatgt
     2881 tgggcagttt gggaataatg ccgggatttt ttaatacatc ccaagcttcc cgtaagatat
     2941 aattcatatt agtacgggcg aatttcctta tttattgccc taaaatcaag ctccgtcttt
     3001 gcgggaagag gtgtcagatt atttgagcca gggttctaat gcctgctatt caatttgaaa
     3061 aaaacaatga atacaaacgt agtgtcaatt ttttttgtga ttcagattat cgaatcgcgt
     3121 tatttcgttg ataaagtcac tcaaccatca cggcagcggc gctttttcta cgcaacgcac
     3181 cgtatctcgc gcgatgagca gttcctcgtt ggtggggann nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnntt ttctacgcaa cgcaccgtat ctcgcgcgat gagcagttcc
     3361 tcgttggtgg ggatgaccca tgttttgagg cgtgcatccg gcgaagagat gacgccttct
     3421 ttgccgccga cggttgcggc attaagatga ggatcgagcg ttaatccaaa aaactccatg
     3481 cctttacaaa tactttcgcg aatatacgcc gaattttcac cgatgccgcc ggcaaatact
     3541 aaagcttcgg gttttccgac ggcggcacag tatgctccga tatatttgcg aatgcgataa
     3601 caatatattt cgatggcaag tttagcgcgg cgatcatcat tttctttcgc ttccgcgatg
     3661 atgtcacgca tatcgttggt aagacctgag atcccgagca aaccggattg tttattgagt
     3721 acggattcga tttcctgcag cgaatatcct tctttatggg agaggaactc caatacagcc
     3781 ggatcaatat cacccgaacg cgttcccata acaagtcctt ccagcggtgt cagacccatg
     3841 gatgtgtcta cggattttcc gccacggatg gcgcaaatgg aggcgccgtt acccaaatgc
     3901 agtgtcatga tgtggacctg ctctttggct tgccccgtca atgtcctgta acgatacgcg
     3961 acataacggt gggacgtacc gtgaaagcca taccggcgca ctttgtagcg gcggtaaaac
     4021 tgatacggaa tagcatagag ataagcttct tcggggatcg tgtggtgaaa tgccgtatca
     4081 aatacggcca cttgcgggat gcctgcgccg aaaatttcgg taaccgcata aatgcctttg
     4141 aggttagccg gattgtgcag gggcgccagt tcaatacaat cttcgatctc ttggatgatt
     4201 tgttcgttga gcaaaatcga gcgggtaaat ttttcaccgc catgcaccac gcgatgacct
     4261 acggcgtgga tatcggataa accgttgatg ccgtttattt ttgtatcttt cccgataatc
     4321 catttgacga ttttatcgac ggccatctta tgatcgcgca tcggtgcggt ttgtttgagc
     4381 gtaggttggc cttcggcttt aaacgaaatg atggattcgc tgccgatacg ttcgatgata
     4441 cccgaggcaa ggcgtgtatc tgcattgtcg cggatctgcg tgaggtccgt attgatgacc
     4501 tgaaatttta cggaagagct gccgctgttg agaactagta tattcatgat atccttta
//
LOCUS       DAOQNQ010000261         4528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_8335_length_4528_cov_6.141516, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000261 DAOQNQ010000000
VERSION     DAOQNQ010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8335_length_4528_cov_6.141516"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1413
                     /gene="radA"
                     /locus_tag="PK754_12985"
     CDS             <1..1413
                     /gene="radA"
                     /locus_tag="PK754_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNB10464.1"
                     /translation="KTKSYFVCQSCGYKSSKSLGRCPECMTWNAMVEEQSATGSSSAR
                     TPDNILKQFGAVDASTYADFSETPQTLDRIEVRPDHRLVSGIAELDRVLGGGIVKGSL
                     VLVGGEPGIGKSTLLLQMAAYQRAKTLYVSGEESVQQIKSRAERIQAPHGGMLVVAET
                     NLHKILQHILQTKPQVVVIDSIQTLYRDEMESAPGSVGQVRECTAILMHLAKTHGISI
                     FLVGHVTKDGMIAGPKVLEHIVDTVLQFEGDTNHLFRILRAAKNRFGSTNEIGIFEMR
                     QEGLAEVANPSELFLSERYLHHSGSVVTPVVEGSRVLLVEVQALASVTNYGMPQRTTS
                     GFDLRRLQLLIAVLEKRARIPLGQYDVFLNIAGGLKTDEPAIDLAVALAVASSHRDFV
                     VDPYTAVIGEIGLGGEVRAVSFAERRVREALKLGFETVILPKSNMKDVSFVSGVNLIG
                     IRRIEEVLDAVTAHTGRTES"
     gene            1415..1762
                     /locus_tag="PK754_12990"
     CDS             1415..1762
                     /locus_tag="PK754_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10465.1"
                     /translation="MYLRDWIIALHEKKRFPAHLCLDTGCTLVIEKIKPLVIVLNALV
                     DNLRSVSTQEIHIDLDAHQKKDQLVLIVHTDRETKPIVKTTITDAIHEIGAEFYEEFM
                     PKQYLKYQLTFSR"
     gene            1779..2486
                     /locus_tag="PK754_12995"
     CDS             1779..2486
                     /locus_tag="PK754_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNB10466.1"
                     /translation="MGRIFEKRKYKMFARFDRMAKTFTRIGKEVAIAVKQGGPDPNTN
                     PRLRAAIANAKAANMPKDRVDAAIKRASSKDEKELQEIIYEGYGPHGIGMIVECATDN
                     PTRTVANIRMYFTKHGGTLGSSGSVAFLFERKGVFKIGKEGVDSDELELEMIDYGAED
                     FAVDDEDIYIYTSFGDFVKMQKALEEKKINVKSAELQYIPSTQTDVTEKQEEDVIKLV
                     EAIEEDDDVQNVFHAMK"
     gene            2512..3057
                     /locus_tag="PK754_13000"
     CDS             2512..3057
                     /locus_tag="PK754_13000"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HNB10467.1"
                     /translation="MGAILKQEPVKLICGLFWNPEVNPETIYRVIEAEWGPMDARSPV
                     FDFDFTAYYHGEMGAVIQKQYVAFEKLVDIEAIADYKIRSNALEDQHFTRDGKRAANV
                     DPGYIADAKLVMATTKNLPHRVYIGKNIYSDLQMMYKKPTFFPIPWTFADVKQPALIE
                     FFNHVRETYIRQIKTLRQNEV"
     gene            3054..3947
                     /locus_tag="PK754_13005"
     CDS             3054..3947
                     /locus_tag="PK754_13005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB10468.1"
                     /translation="MSLALHHVVKSYADTTAVKGLSFEVERGEIFGLIGPNGAGKTTT
                     MRMIIGMIKPDEGWITWLGEPLQASFNDRIGYMPEERGLYRRSRVLDTIVFFAKLKGL
                     SKKTALAEAHRWLEKTELSAYATHRLDALSKGMQQKIQFICTVLHKPELVILDEPFSG
                     LDPVNQNLMRAWIRELADAGMTVIFSAHQMTHVEKLCRRICMMHHGVPVLHDTIAAIR
                     RRHDDGRIRLTGSAEALREVQNTMAWSSLHISDQVLDGIPPAPMSFNQILQRIATLDG
                     IEGIERVHPTLEDIFLKVTKD"
     gene            complement(4195..>4528)
                     /locus_tag="PK754_13010"
     CDS             complement(4195..>4528)
                     /locus_tag="PK754_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10469.1"
                     /translation="SYESIAGEWEGTVFTGEGDTYIEMKLEQDAENVSGYFRAIDQFS
                     DGGGDSGTIEGTMDGDQFTGHAHGSDCSPDIDMTLTHPDTLAGHVHMGACDDGATHAL
                     TVYRKPTL"
BASE COUNT         1202 a         1137 c         1135 g         1054 t
ORIGIN      
        1 aaaaccaaat cttattttgt ctgccaatcg tgcggctaca agtccagcaa atcgctcgga
       61 cgttgcccgg agtgtatgac gtggaatgcg atggtcgaag aacaatcggc cacaggatca
      121 tcctcggcac gcacgccgga taatatactc aagcagtttg gtgcggtgga cgcttcgacg
      181 tatgcggatt tttcggaaac gccgcaaacg ctggatcgca tcgaagtgcg accggatcac
      241 cgcttagtct ccggtatcgc cgagctggat cgtgtgctgg gtggcggcat cgtcaaaggg
      301 tcgcttgttt tggtcggcgg cgaaccgggt atcggcaagt ccacgttgct tttgcagatg
      361 gcggcctatc aacgcgccaa aacactgtac gtctccggcg aagaatccgt gcagcaaatc
      421 aagtcgcgtg ccgaacgcat tcaagctccg cacggcggta tgctggtcgt cgcggaaacc
      481 aatctccata aaatcctgca gcatatttta caaaccaaac cgcaggtcgt cgtgattgac
      541 tccatccaga cgctgtaccg cgacgaaatg gaaagcgcgc cgggcagtgt cgggcaagtg
      601 cgcgaatgta cggccatcct gatgcatctg gccaaaacgc acggcatctc gatatttctc
      661 gtcggccacg tgaccaaaga cggcatgatc gccggtccta aagttttgga acatatcgta
      721 gataccgtac tgcagtttga aggcgatacc aatcatcttt ttcgcatttt gcgcgccgca
      781 aaaaatcgtt ttggttcaac caacgaaatc ggaatttttg aaatgcggca ggaaggtttg
      841 gccgaagtag ccaacccatc ggaattattt ttatcggaac gctacctcca tcattccggt
      901 tccgtcgtaa cgccggtcgt cgaaggttcg cgcgtgcttt tggtcgaagt gcaggcgctg
      961 gccagcgtaa ccaactatgg catgccgcag cgcacgacgt cgggctttga tctacgccgc
     1021 ctgcaactgc tgattgccgt tctcgaaaag cgcgcacgta taccgctcgg gcaatacgat
     1081 gtttttttga atatcgcggg cggattaaaa acggatgaac cggcaattga tcttgctgtg
     1141 gccctggccg tcgcatcaag ccaccgcgac ttcgtcgtgg atccgtatac ggccgtcatc
     1201 ggtgaaatcg ggcttggcgg cgaagtacgc gccgtcagtt ttgccgaacg acgggtgcgg
     1261 gaagcgctca aactggggtt tgaaaccgtg atcctgccga aaagtaatat gaaagacgtg
     1321 agttttgtaa gcggtgtcaa cctgatcggc attcgccgca tcgaagaagt tttagacgcc
     1381 gtcaccgcac ataccggacg cacggagtct tgagatgtat ctgcgggatt ggatcatcgc
     1441 tttgcatgaa aaaaaacgat ttccggcgca tctttgtttg gatacaggat gcacgctggt
     1501 catcgaaaaa atcaaaccgc tggtgatcgt gctgaatgcg ctggtggata atctgcgttc
     1561 ggtgagcaca caggaaatac acattgatct ggatgcgcat caaaagaagg atcaactcgt
     1621 attgatcgta cataccgatc gggaaaccaa accgatcgtg aagacaacga tcaccgatgc
     1681 catccatgaa atcggcgccg agttttatga agaattcatg ccgaagcaat atttgaagta
     1741 tcaattgaca ttttcacgat aaataaagga cgaagaccat gggtcgtatt tttgaaaaac
     1801 gtaaatacaa aatgtttgcc cgttttgacc ggatggcaaa aacgtttacc cgtatcggta
     1861 aagaagtggc cattgccgtc aaacaaggtg gccccgatcc caataccaat ccccgtttac
     1921 gtgcggctat cgccaatgcc aaagccgcca atatgcccaa agaccgcgtg gatgccgcga
     1981 tcaaacgcgc ttcatccaaa gatgaaaaag aattgcagga aatcatctat gaaggatacg
     2041 gcccgcacgg tatcggcatg atcgtcgaat gcgccacgga taatcctacg cgtacggtgg
     2101 ccaatattcg catgtacttt accaaacacg gcggcacgct cggttcttcc ggttcggttg
     2161 cgtttctttt cgagcgtaaa ggcgttttta aaatcgggaa agaaggcgtg gattccgacg
     2221 aactggagct ggaaatgata gactacggcg cggaagactt tgccgtggat gatgaagata
     2281 tctatatcta tacgtccttc ggcgacttcg tcaaaatgca aaaagcgctc gaagagaaaa
     2341 aaatcaacgt caaatcggcg gaattgcagt atattccatc cacgcaaacg gatgtcaccg
     2401 aaaaacaaga agaagacgtc atcaaattag tcgaagccat cgaagaagat gacgatgtac
     2461 aaaatgtttt tcacgccatg aaataaccgc acggaacggg ttttaaaaaa aatgggtgcg
     2521 atcctcaaac aagaaccggt caaactgatc tgcggattat tttggaatcc ggaggtcaac
     2581 ccggaaacga tctaccgcgt gatcgaagcg gagtggggtc ctatggatgc gcgttcgccg
     2641 gtttttgatt ttgattttac ggcgtactat catggcgaga tgggcgcggt gatccaaaag
     2701 cagtacgtag catttgaaaa actcgtggat atcgaagcta tagccgatta taaaattcgc
     2761 tccaatgcgc ttgaagatca gcactttacc cgggacggaa aacgtgcggc caatgtagat
     2821 cccggttata tcgccgatgc caaactcgtc atggcgacaa ctaaaaatct gccgcaccgc
     2881 gtgtatatcg gaaaaaatat ttattccgac ctgcagatga tgtataaaaa accgactttt
     2941 tttcctatcc cttggacttt tgccgacgtc aaacaaccgg cgttgatcga attttttaat
     3001 cacgtgcgcg aaacgtatat ccggcaaatc aaaaccctac gccaaaacga agtatgagcc
     3061 tcgccctgca tcatgtcgtc aaatcctacg cggatacaac ggcggtcaaa ggattgtctt
     3121 tcgaagtgga gcgcggtgag atatttggcc tgatcgggcc caatggcgcg ggtaaaacca
     3181 cgaccatgcg tatgatcatc ggcatgatca aacccgacga aggctggatc acatggctcg
     3241 gcgaaccttt gcaggcgtcg tttaatgatc gcatcggata tatgccggaa gagcgcgggc
     3301 tctatcgccg cagccgcgtg ctggatacga tcgttttttt tgccaaactc aaaggactgt
     3361 ctaaaaaaac agcgttggcc gaagcgcatc gctggcttga aaaaacagaa ctctccgcgt
     3421 acgccacaca tcgtctcgat gcactctcca aaggcatgca acaaaaaata cagtttatct
     3481 gtaccgtgct tcacaaaccg gaacttgtga ttttagatga accgttttcc ggactcgatc
     3541 ccgtcaatca aaatctcatg cgcgcatgga tacgcgaact ggcggacgcc ggtatgaccg
     3601 tgattttttc agcgcatcag atgacacatg tcgaaaagct ctgtcgtcgt atctgtatga
     3661 tgcatcacgg cgtacctgta cttcacgaca cgatcgcagc gatacgacgt cggcacgatg
     3721 acggacgcat acgcctgacc ggttccgcag aagccctgcg cgaagtgcag aacacgatgg
     3781 catggtcatc tctgcacatt tcagatcagg tattagacgg cattccgccg gcccccatgt
     3841 cgtttaatca gatattacaa cgtattgcaa cactcgacgg catcgaaggt atcgaacgcg
     3901 ttcatccaac gttggaagat atttttttga aagtgacgaa ggactagagc gaaggaaggg
     3961 tacatcggaa ggcatccgct ctggattcac agagtttcat tgtttgaatg aaaaaatcac
     4021 tacctcgtcc gattaataat aacgcggact ggctgattga taactacgaa aaaaaataaa
     4081 ctttctcctg cgtacagcat cctcttaacg gctagattcc tctcgttatg aaaggccgta
     4141 agcgcggtga tcacgttctc aacttatttg atttctccca aaccctttac tgcgttataa
     4201 agtcggcttt ctgtaaaccg tgagcgcgtg agtcgcgccg tcgtcgcatg cacccatgtg
     4261 gacatgaccg gctaacgtat cgggatgggt gagcgtcata tcaatatccg gtgaacaatc
     4321 ggaaccatgt gcatgcccgg taaactgatc accatccatc gtaccttcga tcgtaccgct
     4381 gtccccgccg ccgtcactga actgatcaat cgcacgaaaa tagcccgata cgttttcagc
     4441 atcttgttcg agcttcatct cgatgtatgt atcgccttcg ccggtaaata cggttccctc
     4501 ccattcgccg gctatacttt cgtatgaa
//
LOCUS       DAOQNQ010000262         4502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_8415_length_4502_cov_4.992354, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000262 DAOQNQ010000000
VERSION     DAOQNQ010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8415_length_4502_cov_4.992354"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..>4502
                     /locus_tag="PK754_13015"
     CDS             122..>4502
                     /locus_tag="PK754_13015"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNB10470.1"
                     /translation="MRSLRQSGGTLNLTMNDTLVIDTLIQVNGTITGNSPVRIKDWFN
                     WLGGTQSATDSTVATIIQTTALFNSGTHTLSKRTLINRGTISWTLGHWDNNTGAIIRN
                     ESGAIIDATAAGYYINYFGTAPAPKFINNGTFRVSSGSSYVYLNVDAINTNASYQIQS
                     GTLQVSASATNVNTSWTLSNNRFAIFAGGTQTWDADSPISGTGTVRFTGGTHTINSVY
                     NISDSTDVATAVTFNGSAPSVGPYLGISGNATFNTKDSLILSRLTMLSSTATFNTNDS
                     VIVDRMVQSGGIMTGNSPIRVKTRYDWSGGYIYGSDSTVTFINSGIVNLTNNTRQVQK
                     RTFINEGTLNWNAAHWDWDYGAIFINHQGALINITGDWYWNNFPNSGSDPMIYNYGTI
                     HKSSGAGQSTINIPLYDSLATYEVASGTLAVQSNHTSKATNFNLSNGARYRIYGSTQY
                     IDTSNTINGSGILDFNGGTAYVASRIPSVDSVTVSGGTVYFNQPDTLQFSVLSITSGG
                     ILNGSATVKINTRFAFNSGTLSGTDSTNTLIIGSNAVLNASGLNFFSKRTIDNYGTLN
                     WTSSEWRVSNGAVFINRPGAIILTNSGSSYWHQTGSVPRVENYGTIRKSAGANTEWNV
                     ATNHYNGSRVDIKLNKLRFGGVNNATGAVIDIDSAATWQIDAANQTYDSSCTIQGKGM
                     ILGDAFGGTIRLRGIINVTGPTMTLYGSTYYFDGPNGHIIKLPKRIIIGHTFAATNVY
                     FDRSDTAKLEEVWMQNGNFGGTSIILIQDTLKFTNSAIVGTDSSQQIIIAPTAAFLWL
                     GTGSISKRTFNNYGTAIWTTPWGLDQNAVYNNMPGALTDWPLDGNTNWFTGTSNNFNN
                     YGTLRKSGGTGISSFEYYVNNYGTIEAWSGTLFFGTSGTHAGGSFILSPGSTIDFAGG
                     NHSFDASSNITGSGTVLISPTNFTMDGIYNIIGNTTIGGGTVNFDSTFSTSNRLTITG
                     GTVNFRMPSGHLLSVGNRLNVSGGTTNFITTDSLKVDTLLVNGTAGGNNPWFVRHYLQ
                     LTGTLSSNTPAQTLTLSPASVFDYNNGTLFRRVVNNYGTANWNNPVMFDAGAVYNNMP
                     GATTTLATDVNSNWMFAAAKPYLNNYGLLRKSGGTGISSFEYQISNYGTLQVDSGLTI
                     LCYDSLVNFSTGIITGKGVLDVGTRFDNYGTIRPGASPGKLRFTGNINNKSTAAYDAE
                     ISGKNIGQFDELRASGAATLAGTVNVNLLNGFLPALGDTFKIMNYASKSGGFGAIQGL
                     RTGGPYDFVPMYKDTALFLVTADSSNTPPVAVNDIVTASEDVPSTLSVLRNDTDTNGD
                     ALRIVEILSGFPYHGTVVNGSGDTTIVYTSASEYSGSDSLQYVVADGRGGLDTAAVKI
                     AVNSVNDRPDAKDTTTTVTQYSVVRINVLATATDVENQTLSVSAVSRPKFGDAQIDGT
                     SSSVVYIPNMFYTGVDSFKFYVSD"
BASE COUNT         1241 a          964 c         1069 g         1228 t
ORIGIN      
        1 gtattttttg tcttcgtcca gtatgacatt taatgcttct gcaggaaaat tactgacgct
       61 cggcgatagc gtttccatag ccagtggcac gataaacatc acgtcaccgc aaagcatcac
      121 tatgcgaagc ttgcgtcaat ccggcggtac gctcaatttg actatgaacg atacattggt
      181 gattgacaca ttgattcaag ttaacggtac gatcacaggc aattctccgg tacgcataaa
      241 agattggttt aattggttag gaggcacaca atccgcaacg gattcaactg tggcaaccat
      301 aatccagacg accgctctct ttaactcagg tactcataca ctgtctaaac gaacactgat
      361 caaccgggga acgatttcat ggacattagg gcattgggat aataacaccg gcgcaattat
      421 tcgtaatgaa tcaggcgcaa tcatagatgc gaccgctgca ggatactata tcaattattt
      481 tggtacagca ccggcgccga aatttattaa taacgggaca tttcgtgtgt cctccggtag
      541 tagttatgtc tacctcaatg ttgatgccat aaatacaaac gcgtcatacc agattcaatc
      601 gggaacattg caggtatccg ccagtgctac caatgtcaat acgtcgtgga ctttgagtaa
      661 taatcgtttc gccatctttg ctggcggtac gcaaacatgg gatgcggatt cacctatcag
      721 tggtacagga acggttcgtt tcacaggcgg aacgcacacg attaacagcg tatacaatat
      781 ttctgacagt acggacgtcg ctactgcggt gacatttaat ggttctgcgc cgagcgtagg
      841 gccatatctt ggtatttcag gtaacgcaac gtttaataca aaagactcgc tgatattgtc
      901 gcggttgacg atgttaagca gtacggcaac gtttaatacc aacgattcgg tcatagtgga
      961 tcgtatggtt cagtcgggtg ggattatgac aggtaactcg ccgatacgtg tcaaaacgcg
     1021 atacgactgg agcggtggat atatctatgg aagtgattcg acagtaacgt ttatcaatag
     1081 cggcatcgtt aacttaacca acaatacacg tcaggttcag aaaagaacgt ttatcaatga
     1141 aggaacgtta aattggaatg cagcgcattg ggattgggat tacggtgcga tcttcatcaa
     1201 tcatcagggt gcattgatca atatcaccgg agattggtat tggaataatt tccctaatag
     1261 cggctctgat ccgatgatct ataattacgg gactattcac aaatcgtcgg gcgcgggtca
     1321 atctacaatc aatataccac tgtatgattc tttggcaacc tacgaagtgg cgtctggaac
     1381 gttagccgtt cagtccaacc atacatctaa agcaacaaat tttaatttat ccaatggtgc
     1441 acggtatagg atatacggaa gcactcagta catagacact tcaaacacga tcaatggttc
     1501 cggtattttg gattttaatg gcggtacggc ctatgtcgct tcacgaattc cttctgtgga
     1561 tagtgttact gtttcaggtg gaacagtgta ttttaatcag ccggacacac tccaattcag
     1621 tgtgctttcg atcacgagtg gcggcatcct caatggttcg gctacagtaa aaataaatac
     1681 tagatttgct ttcaactctg gtactctctc cggtacggat tccacaaata cattgattat
     1741 cggatcaaac gctgttttga atgcatctgg tttgaatttt ttcagcaaac gtactattga
     1801 taattacgga acgttaaact ggacttcgag cgaatggcgt gttagtaacg gggcggtatt
     1861 catcaatcgt ccgggcgcta ttattttaac caactcagga tctagctatt ggcatcaaac
     1921 ggggagtgtg ccgcgcgttg aaaactacgg aactatccgt aaatcggcgg gtgcaaatac
     1981 ggaatggaat gttgcgacca accactacaa tggttcacgt gttgatatca aactcaacaa
     2041 acttcgtttt ggcggagtta ataacgctac aggtgccgtg attgatattg attccgcggc
     2101 tacttggcaa attgatgcgg ctaatcaaac gtatgatagt tcgtgcacga tacaaggtaa
     2161 aggtatgatt ttaggtgatg cgtttggcgg aacgatacga ctaagaggca tcatcaatgt
     2221 tacgggtcct acgatgacgc tttacggcag cacgtattat tttgacggac cgaatggtca
     2281 tatcataaaa ttaccgaaac gaattattat tggacatacg tttgcggcta cgaatgttta
     2341 ttttgacaga tccgatacgg ccaaactgga agaagtttgg atgcagaacg gcaatttcgg
     2401 aggcacctca ataattctaa ttcaggacac tcttaaattt accaacagcg ctatcgtggg
     2461 aacagattca agccagcaaa taattatcgc tcctacggcg gcattcctat ggcttgggac
     2521 tggcagtatt tctaaaagga cattcaataa ttacggaacg gcgatttgga caacaccttg
     2581 gggacttgac cagaacgcag tttataataa tatgccgggg gctttgaccg attggccgct
     2641 ggatggaaac accaactggt ttaccggaac ttcgaataat tttaataatt atgggacact
     2701 tagaaaatcc ggtggtaccg gtatatcaag tttcgaatat tacgtgaata actatggtac
     2761 aatcgaagct tggtcgggta cgttgttttt tggaaccagc ggtacccatg ccggaggtag
     2821 ttttatactt tcgccaggct cgacgattga ctttgccgga ggaaaccact cttttgacgc
     2881 gtcgagcaat atcacaggca gtggtaccgt actcatcagt cctactaatt ttacgatgga
     2941 cggtatatat aatattatcg gtaatactac gatcggtgga ggtactgtca attttgacag
     3001 cacattcagt acatccaaca ggctaaccat caccggcggt acggttaatt tcagaatgcc
     3061 atccggtcat ctcctgagtg ttggaaatag actgaatgtt tccggcggta cgacaaattt
     3121 tattacgacc gactccctga aagtagatac gctgctggtt aatggaaccg ccggtggaaa
     3181 taacccgtgg tttgttcgtc attatttgca gttgacaggt acattgagtt ccaatactcc
     3241 tgcacaaaca ctgacattgt cgccggcatc agtttttgat tataataatg gaacgctgtt
     3301 ccgacgcgta gtcaacaact acggtactgc caactggaat aatcctgtaa tgtttgacgc
     3361 aggagctgtg tataacaaca tgcccggtgc gacgacgaca ctggcaaccg atgtgaattc
     3421 taattggatg tttgcagctg ccaaaccgta tctaaacaat tacggattgc tgcgcaaatc
     3481 cggcggcacg ggcatttcga gttttgaata tcagatcagt aattacggta cattacaggt
     3541 tgattccggg ttaacgatct tgtgttatga cagtcttgtt aacttcagta ccggtattat
     3601 caccggcaaa ggtgttcttg atgtcggtac gcgttttgat aattacggta caatacgtcc
     3661 cggcgcttcg cccggcaaac tgcgtttcac cggaaacatc aataataaga gtacggctgc
     3721 ttatgatgcc gagattagcg gaaaaaatat aggccaattt gatgaactgc gcgcaagcgg
     3781 cgccgcgaca ctcgcaggaa cagtaaatgt aaatttactt aatgggtttt tgccggcgct
     3841 tggcgacacg tttaaaatca tgaattatgc gagtaagtcc ggaggtttcg gagcgataca
     3901 aggattgcgt accggcgggc catatgattt tgtaccgatg tataaagaca cggcgctttt
     3961 tcttgtgact gcagattcgt ccaatacacc tccggtagcg gtcaatgaca tcgtgacggc
     4021 ttcggaagac gtcccatcaa cactttctgt tttacgtaac gatacggata ccaatggtga
     4081 cgcgcttcgt attgtcgaaa ttctctccgg attcccgtat cacggtaccg tggtaaatgg
     4141 tagtggcgac acgacgattg tttatacgtc cgcttcggag tattccggtt cggatagtct
     4201 gcagtatgtg gttgcagatg gtcgcggcgg tttggatacg gcggcggtaa aaatcgcggt
     4261 taacagcgtt aatgatcgtc ctgacgcaaa agatacaaca acgacggtca cacaatatag
     4321 cgtcgtacgc atcaatgtac tggcaacagc gacggacgtc gaaaatcaaa cactctcggt
     4381 gtcagcggta tcgcggccta agtttggcga cgcacagatt gatggtacat cctcctctgt
     4441 tgtgtacata ccaaacatgt tttataccgg cgtggatagt ttcaaatttt atgtctccga
     4501 cg
//
LOCUS       DAOQNQ010000263         4478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_8486_length_4478_cov_5.933755, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000263 DAOQNQ010000000
VERSION     DAOQNQ010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8486_length_4478_cov_5.933755"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1067
                     /locus_tag="PK754_13020"
     CDS             126..1067
                     /locus_tag="PK754_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNB10471.1"
                     /translation="MKIGFAYAPIFLEHRMYAGHHPECPERLGAITQAIAEAKLNDTL
                     ENLHIKPADESLLVEVHSEVHIDRIKEEASRAPAMIDGDTYVAHASYEAARMAAGAVV
                     VAVDAVCSGQLTRAFCAVRPPGHHAEKEKAMGFCLFNNVAVGATYARRAYGLQRIAIV
                     DWDVHHGNGTQAIFYEDPTVLFISTHESPLYPGTGQHTEIGNGAGKGYTLNIPMHAGD
                     GDMEMETVFDKQIIPALDQFKPELLFISAGFDAHARDPLANMTVTSEGFGKLTRLVVQ
                     AAEKHCQGRVISVLEGGYHLEALGASVVSHIRQLAHE"
     gene            1060..1728
                     /locus_tag="PK754_13025"
     CDS             1060..1728
                     /locus_tag="PK754_13025"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNB10472.1"
                     /translation="MNKRVSPPWALLLDVTLCIFLLFFGAPIYGQTLDSNVSATTVLP
                     QVSRDSLRTFLLGKPHGNEKKSVPRMTFTGDTLFRSPWGAVGRSFLVNGWGQWYNERP
                     MKGLVFLGADAAMIYFYRVKNRKVDKIQAQRKRIDRQLVNDPFLTPEQKSILRSRFSN
                     LTSDLDGALTDRNLYGWLFAISHLMGMIDAYVDAHLFNFDEKMELAYGTDGRYFNVAM
                     RITF"
     gene            1738..2670
                     /locus_tag="PK754_13030"
     CDS             1738..2670
                     /locus_tag="PK754_13030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB10473.1"
                     /translation="MKAQKKKIMPAASDAKKRREPKNAERIQKILMNSDKMASLGQLI
                     AGVAHELNNPISFIESNVIFLEKYCNDLRTVITMLESSQTMDADVRAKVEAYKKEMEW
                     DFAMGDMENILKSFNEGATRIRSILASLSAFSRGNRIAREEMNIHEAIDNTINLLRYK
                     SRHVVEFETKYKANPYIIANRGEINQVLMNLIINAIHAVQHKFENGGKGAIYIHTHSG
                     ENFVYIDIKDNGEGIPDIYKNEIFKAFFTTKKEGEGTGLGLPISKEIVEKHGGAIEFE
                     SRAGIGTCFTVKLPFGTDEETDVPKDTHNQGKKS"
     gene            2667..3887
                     /locus_tag="PK754_13035"
     CDS             2667..3887
                     /locus_tag="PK754_13035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNB10474.1"
                     /translation="MSVATVASPSMTEPNKIMHDILVVDDEVDNLDLLKRTFRREYNV
                     YSANSAAEALKLLDEREFAVIVSDQRMPEMTGVEMFQKAREKYPQTIRILLTGYTDIN
                     ALVDAINMGHVYRYVTKPWSREEIVMTVKRAVEHYETTKQNARLLDELKIKNEELDRN
                     NKQLKRLDELKTRFMVISSHELRTPASIISGNLELLMSGAMGEVPPEHQEIVTNAYKG
                     TTRLIDLIEDVLSVMRSDSHNLKLNLSAYSPREVLEEVISSYRAFLHERRQTVQNELP
                     ELTIEGDRDRMFHVFANALSNAMKYSRDGETIGVSGKLNGDHMRITFRDAGIGIPPEE
                     LERIFEKFYQLGDADQHRTSKHKFMGGGSGLGLTIVRGIVEEHHGRVWAESEGKDKGA
                     ALQIELPLQQPVAG"
     gene            3903..>4478
                     /locus_tag="PK754_13040"
     CDS             3903..>4478
                     /locus_tag="PK754_13040"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNB10475.1"
                     /translation="MPQSLSYVHSVHLALTRRCGAQCNYCTFKQSDSPLMTFDEIEQV
                     IRKQKPTGISTVVLGAGQSLERLADLPALWNDQGYASFIHYVQDVCRIVLEHQLLPVV
                     DIGPLTYAQLEMLRPFVPTIHLALENINTDFRATVQQNKSADDIMETFSDAGILGIPV
                     TTGLLFGAGESIDDGLATLNALDEIQKRYHNL"
BASE COUNT         1228 a          961 c         1152 g         1137 t
ORIGIN      
        1 gacttgcgtt tgtcggcggt tattgaaaaa gtggagagcc ttttgatgat tacgcagctt
       61 ctcaagattt ttaaaaccta caaaaccgtt gaagaagccg tacaaagttt ttcttcacag
      121 gatgtatgaa aatcggattt gcctacgcgc ctattttttt agaacatcga atgtatgccg
      181 gtcatcatcc ggaatgcccc gaacgcctcg gggccatcac gcaggctatc gcagaggcca
      241 aattaaatga cacactggaa aatttacaca taaaacctgc cgacgaatca ttattggtgg
      301 aagtgcatag tgaagtccat attgatcgta taaaagaaga agcgtcccgc gcgccggcga
      361 tgattgatgg cgacacctac gtcgctcatg cttcgtatga ggccgcgcgt atggccgcag
      421 gcgccgttgt agtggccgta gatgccgttt gctccggtca gctcactcgc gcattttgtg
      481 cggtgcgccc gccgggccat catgccgaaa aagaaaaagc gatgggtttt tgtttgttta
      541 ataatgtggc tgtcggggcg acgtatgcgc gccgtgccta cggattacaa cgcattgcga
      601 tcgtagattg ggatgtgcac catggtaatg gtacgcaagc cattttttat gaagacccga
      661 cggtactttt tataagtact cacgaatcgc ccttgtatcc cggcacaggt cagcataccg
      721 aaatcggcaa cggagccggt aagggttaca cgctcaacat acctatgcat gccggtgatg
      781 gtgatatgga gatggagacc gtgtttgata aacaaatcat accggcattg gatcaattca
      841 aaccggagct tttattcatc tcagcaggat ttgatgcaca tgcccgggat cccttggcga
      901 atatgacggt gacttcggag ggatttggaa aattaacccg tttagttgta caggcggcgg
      961 agaaacattg tcaagggcgc gtgatttctg tattggaagg tggttatcat cttgaagcat
     1021 tgggcgcatc ggtcgtctct catatacggc agctagccca tgaataagcg cgtatcgccg
     1081 ccgtgggcat tgcttttgga tgtcactttg tgcatatttt tattgttctt tggcgcaccg
     1141 atatacggtc aaactttaga ttccaatgtg tcggctacga ccgttttgcc gcaggtaagt
     1201 cgcgattcgt tgcgaacgtt tcttttggga aagccacatg gaaacgaaaa aaaatcagtg
     1261 ccgcggatga cttttacggg ggatacgtta ttccgttcgc cgtggggcgc cgtggggcgc
     1321 agttttttgg tcaacggctg gggacagtgg tacaatgaac ggccgatgaa aggattggtt
     1381 tttcttggcg ccgatgcggc gatgatctat ttttaccgtg ttaaaaaccg gaaagtagat
     1441 aagatacaag cccaacgaaa acggatagac cgtcaattgg ttaacgatcc ttttctcacg
     1501 ccggaacaga agtccatttt gcgatcaaga tttagcaatc tgacaagcga tttggatggt
     1561 gcattgacgg atcgcaattt atacggatgg ttatttgcca tttcgcattt gatgggaatg
     1621 attgatgcgt atgtggatgc gcacttgttt aattttgatg aaaaaatgga acttgcttac
     1681 gggacggatg gccggtattt taacgtggct atgcgtataa cattttaacg ggacattgtg
     1741 aaggcccaaa agaaaaaaat aatgcctgcc gcttccgacg ccaaaaaacg ccgggaaccc
     1801 aagaatgccg aacgtattca gaagattttg atgaattcgg ataaaatggc atcactgggt
     1861 caactgatag cgggcgtagc gcacgaactc aacaatccga tcagctttat tgagagcaat
     1921 gttatttttt tagaaaaata ctgcaacgac cttcgcacgg taatcactat gttagaatcg
     1981 tcgcaaacga tggatgctga tgtgcgggct aaagtcgaag catacaaaaa agaaatggaa
     2041 tgggattttg ccatgggcga catggaaaat atcctcaaaa gtttcaatga aggcgctacc
     2101 cgcattcgat ccattcttgc cagcttaagc gcattctccc ggggcaatcg cattgcacgg
     2161 gaagaaatga atatacacga agcgatcgat aacacgatca atcttttgcg atacaaatcc
     2221 cgtcatgtcg tagaatttga aaccaaatat aaagctaatc cttacattat cgccaatcgc
     2281 ggtgagatca atcaagtgct gatgaatctc atcattaatg cgatacacgc cgtgcaacat
     2341 aaattcgaaa acggcggtaa aggtgcgatt tacattcata cccattcggg tgaaaacttt
     2401 gtttacatag acattaaaga taacggtgaa ggcatcccgg atatttataa aaacgaaatt
     2461 tttaaagcat tctttacaac gaaaaaagaa ggcgaaggaa ccggtttggg tttgccgatt
     2521 tccaaagaga tcgtcgaaaa acacggtggt gctatcgaat ttgaaagccg tgccggtatc
     2581 ggcacttgtt ttacagtaaa acttcctttc ggtacggacg aagaaaccga cgtgcctaaa
     2641 gatacgcata accaagggaa aaaatcgtga gtgtagcgac ggttgcatct ccgtctatga
     2701 cggaacccaa caagatcatg catgatattt tggtggtaga tgatgaagtg gataatcttg
     2761 atctgctgaa acgtacgttt cgccgggagt acaatgtgta ctcggccaac agcgctgcgg
     2821 aggcgttgaa gcttttggat gaacgcgaat ttgccgtgat cgtcagcgat cagcgtatgc
     2881 cggaaatgac gggcgttgaa atgtttcaga aagcgcgcga aaaatatccg cagaccattc
     2941 ggattttgct taccggttat accgacatca atgcgctggt agatgcgatc aacatgggtc
     3001 acgtgtatcg ctatgtgacc aaaccgtgga gtcgtgaaga aatcgtcatg accgtcaagc
     3061 gcgcggtcga gcattacgaa accaccaaac aaaatgcgcg gctcttggat gaattgaaaa
     3121 tcaaaaacga agaactggat cgtaataaca aacaactgaa acgcctggat gaacttaaaa
     3181 cacggttcat ggttatttca tcgcacgaat tgcgcacacc cgcctccatc atttccggaa
     3241 atctggagtt gcttatgtcc ggtgcaatgg gtgaagtgcc cccggaacat caggagatcg
     3301 ttaccaatgc atataaaggc acgacgcgtc tgattgattt gatcgaagat gttttgtccg
     3361 ttatgcgttc ggattcgcat aatctcaaac tcaatctttc ggcgtattca ccgcgtgagg
     3421 tgctggaaga agttatttcc agttacaggg cttttttgca tgaacgccgg caaacggtac
     3481 aaaacgagtt accggaattg accattgagg gtgatcgtga caggatgttt catgtatttg
     3541 ccaacgcgct cagcaacgcg atgaagtatt cccgcgacgg tgaaaccatc ggggtatcgg
     3601 ggaaactcaa cggcgatcat atgcgcatta catttcgcga cgcgggtatc ggtattcctc
     3661 cggaagaact ggagcgtatt tttgaaaagt tttaccaact cggtgatgcg gatcagcatc
     3721 ggacgagtaa acataaattt atgggcggcg gcagcggtct ggggcttact atcgtgcgcg
     3781 gtatagtgga agaacaccac ggtcgtgtat gggcggaaag cgaaggcaaa gataaaggcg
     3841 ctgcacttca aatcgaatta cctttgcaac aaccggtagc cggataaacg aaagagctca
     3901 acgtgccgca aagtttgtct tacgttcata gtgttcatct tgcactcacg cgccgatgcg
     3961 gggcccaatg caattattgt acattcaagc aatcggattc gccgttgatg acgtttgacg
     4021 aaatcgaaca ggtgattcgc aaacaaaaac caaccggcat ttcaacggtg gtgttgggcg
     4081 ctggtcaatc gctagaacgc ttggcggact taccggcgtt gtggaatgat caggggtacg
     4141 catcgtttat acactacgtg caggatgtgt gccgcatcgt actggaacat caacttttgc
     4201 ctgtggtgga tattggtccg cttacatatg cccaactgga gatgttgcgt ccgtttgttc
     4261 ctacgattca tttagctttg gagaatatca atacggactt tcgcgcgacg gtacaacaaa
     4321 ataaaagcgc cgatgacatt atggaaactt tttccgatgc gggcattctc ggcataccgg
     4381 ttacgaccgg tttacttttc ggcgcgggcg agagtattga tgacgggttg gcgacgctca
     4441 atgcgttgga cgaaattcag aaacgttatc acaacctg
//
LOCUS       DAOQNQ010000264         4477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_8488_length_4477_cov_5.380371, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000264 DAOQNQ010000000
VERSION     DAOQNQ010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8488_length_4477_cov_5.380371"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(353..1405)
                     /gene="asd"
                     /locus_tag="PK754_13045"
     CDS             complement(353..1405)
                     /gene="asd"
                     /locus_tag="PK754_13045"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNB10476.1"
                     /translation="MKKIKVGILGATGTVGQRFIELLHAHPYFEITALAASERSVGKK
                     YNDLNTWKLDSVLPSRIGEMIVQPCSPGMDARVMFSALDSSVAGAIEIEFARAGYIVV
                     SNAKNYRMDEDVPLLFPEVNADHTRLIEIQKKNRGFDRGYIVTNSNCAIMGFVPIVHV
                     LHQHYGVVSVDVTTLQAISGAGYPGVPSMDILGNVIPYISGEEEKIETEPLKIWGQML
                     QDRIQFASIAISASVHRVPVLDGHLASVTAKLKQLPAIGDIKQKLSEFKSVPQELNLP
                     LAPMRPIVVIEGDDRPQPRRDVTLEKSMAASVGRIRYDRSGNLKFSCLVHNTVRGAAG
                     GAVLNAELLHAKGLLE"
     gene            complement(1628..2167)
                     /locus_tag="PK754_13050"
     CDS             complement(1628..2167)
                     /locus_tag="PK754_13050"
                     /inference="COORDINATES: protein motif:HMM:NF025595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HNB10477.1"
                     /translation="MADEKEQKDELLEKGAALSTAIIGVFLSISSILGGNANDDIMIH
                     RGLANNQWSYFQAKSTKQALYEVNRKMFETQLADPSLAESYKKEIQAKLEKFDSEVAR
                     YDTEKKEIKAKAEEHEKLSDQAAEIEDKYDYAEGFYQIAIILAAVAMIAGNRKLWFFS
                     IVLGSIAIAITGFAFFVSL"
     gene            complement(2208..>4477)
                     /gene="topA"
                     /locus_tag="PK754_13055"
     CDS             complement(2208..>4477)
                     /gene="topA"
                     /locus_tag="PK754_13055"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNB10478.1"
                     /translation="LGKSFHVEASVGHIKNLPSNKLGIDIENGFTPEYRIIDGKQDVI
                     RKLKTLAASTKNVFIATDPDREGEAIAWHIAGEIEKHNPSISRVLFNEITKSGVEEAM
                     RTPTKIDPKLVQSQQARRVLDRIVGYGVSRYLWGVVRRGTSAGRVQSVALQLVCKREE
                     EINAFVPQEYWSIHAKVQDEPTEAFLAHLHSIDGKKVDIASGDVAHRFVEDIRKKNFL
                     IKDIRKREARRNPSAPFVTSSLQQEASRKLGFSPKLTMMLAQQLYEGIDLGSEGLTGL
                     ITYMRTDSTRVADTALVSVREYIMGSYGREYIPHTPNQFKSKKANVQDAHEAIRPTNV
                     KFTPKTVNKYLTPQQAKLYELIWNRFVASQMTPAELDQTTIDISADEYLFRTTGSVVT
                     FKGFLQVYEEGRDEGDKTQDNGEEEDNSGLLPANLKINDVLKLHELLPEQHFTKPPAR
                     FTESSLIKELDNIGIGRPSTYAMIVGTIVEREYAELRERKLYATDLGKSVNKVLNDHF
                     NALFNVQFTAEMEEELDKIESGELGYKKVLDDFYNPFVKNMKDVESKKDAIKESVQEK
                     TDETCEICGKPMVIKWGRFGKFMSCTGYPECKNIKKVSKDGEAAPPPEVTDEKCPNCG
                     SPMVVRQSRGGKFLGCSNYPTCKTAKAHPDDIIKDVICPKDGGQLIRRRSRYGKFFYG
                     CANYPNCDFVSWNEPVATRCPHCDAAFMVKKTSKRKGDFLLCMTCNHEEILPADTSVM
                     KEQPENESVSFNEGE"
BASE COUNT         1156 a         1099 c          915 g         1307 t
ORIGIN      
        1 atgcatcggt tgccaaggct tcacagggcc tgatccctca gcctttcgcg ataagagcgt
       61 atgtcgttag ttgtttaagt cattttattc tttgtttgag tgtaaaacaa aaagcccttc
      121 cgttttatcg gaagggcttg aagtaaaaat attctactta tttgctataa atggatatag
      181 cccttccgcg tacaggtgca catgcacatc tgcatcatgg tgggcattat attctgagct
      241 gtttttgtgt tgctaaagaa aatcataatg aaataatata tacgtcgcac cccgcccttg
      301 tcaagaggaa tcatcgtttt gattaaaaaa cttttcattt atttgttttt ttttattcca
      361 ataaaccttt agcgtgaagc aattcggcat taagtactgc tcccccggcc gcaccacgaa
      421 ccgtattatg aaccaaacaa gaaaacttga ggttgcctga acggtcgtat cgaatacggc
      481 cgaccgacgc ggccattgat ttttccaaag taacgtcacg gcgcggttgc ggacggtcat
      541 cgccctcaat gacgacgata ggcctcatcg gcgcaagcgg taaattcaat tcctgcggca
      601 cgcttttaaa ttctgataat ttttgtttga tatcgccgat tgcaggcagc tgttttaatt
      661 tcgctgttac actcgccaag tgcccgtcca aaacgggaac ccggtgtacg gaggcactga
      721 tcgcaatcga agcgaactga atgcgatcct gtaacatttg tccccaaatt tttaaaggtt
      781 cggtttcaat tttttcttct tcaccggaaa tatacggaat cacatttcct aaaatatcca
      841 tcgaaggcac gcccggataa ccggcgccgg aaatcgcttg cagtgtggtc acatctaccg
      901 atacgacacc gtaatgctga tgcaatacat gcacaatagg aacaaacccc atgatcgcgc
      961 agttggaatt ggttacaata taccctcgat caaaaccgcg attctttttc tgaatttcta
     1021 ttaaccgcgt gtgatctgca ttgacctccg gaaaaagcaa aggtacatct tcatccatcc
     1081 ggtaattttt ggcattactc acgacaatat aaccggcgcg ggcaaactct atttcgatcg
     1141 caccggcaac cgaagaatcc aatgccgaaa acatcacacg cgcatccatt cccggagaac
     1201 atggctgcac gatcatttcc ccgatacgcg aaggcaaaac gctgtctaat ttccatgtat
     1261 tgagatcgtt gtattttttc ccgacggatc gttccgaagc cgccaatgca gtaatctcaa
     1321 aatatggatg ggcgtgaagc aactcgatga aacgctgacc cacggtgcct gtcgctccca
     1381 aaattcctac ttttattttt ttcacattta ctcctaaaaa aattagccgg gctgtgaacc
     1441 cggctgttat taaaaatata aaaataacta gatcagacgt tcagcgccgc attcgatggt
     1501 gcataaccat acgttttcgc ttaccgttag acattcggca cgaaagtatg ttcgttaatt
     1561 ttagcgctga aatcttcatt tattaaatcg ttttttatta tcaatcattc tgtaatacta
     1621 acttatacta aagagaaacg aaaaaagcaa atccggttat tgcgatagcg atagaaccca
     1681 gtactatact gaagaaccaa agtttgcgat tgcctgcaat cattgccaca gccgcaagaa
     1741 tgatggctat ttgatagaaa ccttcggcat agtcgtattt atcttcgatc tcagcggcct
     1801 gatcggaaag tttttcatgt tcttccgctt tggcttttat ctcttttttt tcggtatcat
     1861 aacgcgcgac ttccgaatca aacttctcaa gttttgcctg aatttctttt ttatagcttt
     1921 cggccaggga cggatcagca agttgcgttt caaacatttt acgatttact tcatagagcg
     1981 cttgtttggt gctcttcgct tggaaatacg accattgatt attggccaaa ccgcgatgaa
     2041 tcatgatatc atcattggca ttgccgccca aaatggaaga aatggataaa aatacgccga
     2101 taatcgccgt actcagcgcc gcgccttttt ccaatagttc gtctttttgt tccttttcat
     2161 ccgccataaa tatttctcct cctaaatatt aaactgaacc ttaaaattta ttccccttca
     2221 ttaaaggaaa ccgattcgtt ttcaggttgt tctttcatca ccgacgtatc cgccggcaaa
     2281 atctcttcgt gattgcaagt catacataaa aggaaatcac ctttacgttt gctggtcttt
     2341 ttgaccataa acgcggcatc gcaatgcgga caacgtgtcg ctaccggctc attccacgat
     2401 acaaaatcac aattgggata attcgcacaa ccgtagaaaa acttaccata ccgtgaacgt
     2461 cggcgtatca actgaccgcc gtctttagga caaatcacgt ctttgataat atcatccgga
     2521 tgcgctttag ccgttttaca agtcgggtaa ttggaacatc cgagaaactt gccaccgcgg
     2581 ctttgacgta caaccatagg gcttccgcag ttcggacatt tttcatcggt cacttccggc
     2641 ggtggcgcgg cttcgccgtc ttttgatact tttttgatat tcttacattc cggataaccc
     2701 gtacaagaca taaattttcc gaagcgcccc catttgatca ccatcggttt accgcagatt
     2761 tcgcaggtct catccgtttt ttcctgcacc gattctttga tcgcatcttt tttactttcc
     2821 acatccttca tgtttttcac gaaaggattg tagaaatcat cgagtacttt tttataaccc
     2881 agttctccgg attcgatctt atccagctct tcttccatct ccgcggtaaa ctgtacgtta
     2941 aaaagcgcat taaaatgatc gttcagcact ttattgacgc tcttaccgag atcggtcgca
     3001 tacagcttgc gctcgcgcag ttcggcgtat tcgcgttcca cgatcgtacc gacgatcatc
     3061 gcgtaagtac tgggacggcc aatgccgata ttgtctaatt cttttatgag cgaactttcc
     3121 gtaaaacgtg caggcggttt ggtaaaatgc tgctccggca aaagttcatg caacttgagt
     3181 acatcattga tcttcagatt ggccggcagt aaaccgctgt tatcctcttc ttcaccgttg
     3241 tcctgcgtct tatcgccttc gtcgcggcct tcttcatata cctgcaaaaa ccctttaaac
     3301 gtcacaacgg aacctgtagt acgaaaaagg tattcgtcgg cggaaatatc aatggtcgtc
     3361 tgatccagct cagccggagt catctgcgaa gccacaaaac gattccaaat caattcatac
     3421 agtttggcct gttgcggcgt gagatattta ttcacggttt taggcgtgaa tttcacattt
     3481 gtaggacgta tcgcttcgtg tgcatcttgc acattggctt ttttactttt gaactgatta
     3541 ggcgtatgag gaatatattc gcggccatag ctgcccatga tatattcacg caccgacacc
     3601 agcgctgtat cagccacacg cgtagaatcc gtacgcatgt aggtaataag acccgtaagt
     3661 ccctcgctac caaggtcaat accttcatac agctgctgcg cgagcatcat ggtcagctta
     3721 ggtgaaaaac cgagctttcg tgaagcttcc tgttgtagcg agctcgtaac aaaaggcgcc
     3781 gagggattac gtcgcgcttc acgcttgcga atatctttga tcagaaaatt tttcttacga
     3841 atgtcttcga caaaacggtg tgcaacgtcg ccgctggcta tatctacttt tttcccgtct
     3901 atggaatgca gatgtgccaa aaaggcctct gtcggttcat cctgcacttt ggcatggatg
     3961 ctccaatatt cctgaggaac aaaagcattg atctcttctt cacgtttgca aaccagttgc
     4021 aaagcaacgg actgtacgcg acctgccgat gtcccgcggc gaacaacacc ccacaggtac
     4081 cgactcacac cataaccgac gatgcgatcc agcacacggc gcgcctgctg cgactgaact
     4141 aatttgggat caattttcgt cggtgtgcgc attgcctctt cgacaccgct tttggtgatt
     4201 tcattaaaaa gtacacgcga tatgctggga ttatgctttt cgatttcacc ggcgatgtgc
     4261 catgcgatcg cttcaccttc gcgatcaggg tcggtcgcga taaatacgtt ttttgtggaa
     4321 gcggccaacg tttttagttt acggatgaca tcctgcttgc cgtcaataat gcggtactcg
     4381 ggggtaaaac cgttttcgat atcaataccg agtttattcg acggaagatt tttgatgtgc
     4441 ccgaccgaag cttcgacatg aaatgatttg cctaagt
//
LOCUS       DAOQNQ010000265         4450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_8578_length_4450_cov_4.304209, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000265 DAOQNQ010000000
VERSION     DAOQNQ010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8578_length_4450_cov_4.304209"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            489..1862
                     /locus_tag="PK754_13060"
     CDS             489..1862
                     /locus_tag="PK754_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNB10479.1"
                     /translation="MLSSSKILIIDDDEDVLLAARLFLKQHVETVRTEKNPERIPHLL
                     KEEAWDVIFLDMNFTRDVSSGIEGFQWLERILQIKPDAVVIMITAYGDVDMAVRAIKA
                     GAMDFVLKPWQNEKLLATLSAAVRLRVSRREADQLRDREKQLVQAMESPMAEFIGQSE
                     AMKKVFATIEKVAATDANVLILGENGTGKELVARALYHRSHRKDKPFITVDLGAISET
                     LFESELFGHVKGAFTDAKEDRVGRFEVAGGGTLFLDEIGNLSLALQSKLLTAIQKNEI
                     TRLGSNKPIAVNARLICATNMPIKQMAEEKTFRQDLLYRINTIEIQLPPLRERKPDIH
                     LLTDHFVKLYAKKYQKPAHTLSPSAIHKLENYPWPGNVRELQHTIERAVILCDAQLMT
                     ADDFALELGRTRTEPLQVDDYNLEEIEKALIRKVIEKHNGNISQAAKELGLTRTSLYR
                     RLEKHGI"
     gene            1852..3219
                     /locus_tag="PK754_13065"
     CDS             1852..3219
                     /locus_tag="PK754_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB10480.1"
                     /translation="MVSKFRLMVIVRILLLTATINVWIYVMRATELYVTMALLAVIVV
                     YQIGSMIVYVEKTNRDLRRFLEAVRHEDFTQTFAGAGLGSSFDELKSAFNEIITNFRK
                     TRAEKEEHFRYLQTVVQHIGVGLIAFTSDGTVELMNTAARRLLRTGQWLPTVDLKNIH
                     SIKTLSPSLADAFINLKTGDKAIIKVEMRSEFMQLMVQATEFRLREQLYRLVSLQNIQ
                     TELEEKEMEAWQNLIRVLTHEIMNSVTPISSLASTVNDLITKTYENENQNKPDPDTLS
                     DIHGALTTIQNRSQGLIHFVDAYRNLTKIPKPTFRLVTVEELFARIEHLMRPRMAEKK
                     ILFSKSIDPQSLEITADSILIEQVLINLLVNAMHAVEGQSRPEIWLRADMDENGRVCI
                     HVTDNGYGIIEEALNKIFIPFFTTKPDGSGIGLSLSRQIMRLHRGSITVQSKPHVKTV
                     FTLRF"
     assembly_gap    3226..3325
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3326..3779
                     /locus_tag="PK754_13070"
     CDS             <3326..3779
                     /locus_tag="PK754_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10481.1"
                     /translation="MNHTIVRFLLLVLLLNVVRYIAAFPLEMMLVFPGLFGAMESAPG
                     VFNVSFTTGDWITSYVYNFILWTVSVFIFHRMAPVVGGKWIIKSVKIFFLPWLLFVSV
                     SAIYMNHYAHTKMFYVYTMLDSVITFGIVAVANGLLYPRIMGKSSSVG"
     gene            3994..>4450
                     /locus_tag="PK754_13075"
     CDS             3994..>4450
                     /locus_tag="PK754_13075"
                     /inference="COORDINATES: protein motif:HMM:NF024886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HNB10482.1"
                     /translation="MKHKIIRIETVTRNSENHVLIRFGFDEELITLVKQLPGIRWSAA
                     DKAWQMPNTKENVNRIFLLFRGKAWVDYNALKSQKPENTPARKDTNAGMILRKQLNES
                     VLKKIDQFVMWLQSKRYGENTIKTYTDALRTFLRFHHGIPLNEITNDHII"
BASE COUNT         1192 a          990 c         1042 g         1126 t
ORIGIN      
        1 tgtcttaccg tttccttaaa aggttaccgt tccggcgcaa gcatgtcgtt cccgcgaaag
       61 cgggaaccta cggagaagaa ataaattcgt taaccacaat gctaaacctg tggtcacata
      121 gacacccgcg tacgcgggtg agacaatacg aataacttca cgcggccacg cccgtgaaag
      181 catgtcgttc ccgcgaaagc gggaacccac taaaaacagg acgttgcatc tttaagtatg
      241 gatttgatgg attaaaacgg ttgttccaat gtagttattc gcattaacta ttaattttcg
      301 cacatttcac ggaaattaaa aacaaacaaa acagcgcgcc atgcgtatgt aaacgtgttt
      361 ctgtaaaaac gaacgaaacg gttttttggt aataaatatt ttgtgaatgc ggccacggtt
      421 ctttagacca acacattacc aatcgttaga aatgcatcga taatcatcct gaaaatataa
      481 ttacagctat gctttcttcc tccaaaattc tgatcataga tgacgacgaa gatgtgcttt
      541 tggccgcgcg tctttttctg aagcaacacg tggaaaccgt tcgtacggaa aaaaatccgg
      601 agcgcatccc gcacttgctc aaagaagaag cgtgggatgt gatttttttg gatatgaatt
      661 ttacccgcga tgtgagcagc ggaatcgagg gttttcagtg gcttgagcgt attttgcaaa
      721 tcaagccgga tgccgtcgtg atcatgatca ccgcctacgg ggatgtcgat atggccgtgc
      781 gggcgatcaa agcgggggcg atggattttg ttctcaaacc ctggcaaaac gaaaaactct
      841 tagcgacgtt atccgcggcg gtacgactgc gcgtttcgcg gcgtgaggcc gatcaattgc
      901 gtgatcgcga aaaacaattg gtgcaggcga tggagtcgcc gatggcggag tttatcggtc
      961 aaagtgaagc gatgaaaaaa gttttcgcca cgatcgaaaa agtcgccgcg accgatgcca
     1021 atgtgctgat cctcggtgaa aacgggacgg gtaaagaatt ggtcgcacgc gccttgtatc
     1081 atcgttccca tcgtaaggac aaacctttta tcaccgtcga tctcggcgcc atcagcgaaa
     1141 cgctttttga aagcgaactg tttggtcatg tgaaaggcgc ttttaccgat gccaaagaag
     1201 atcgtgtggg tcgttttgaa gtggccggcg gcggtacatt gtttttggat gagattggaa
     1261 atctttcgtt ggccttgcaa tccaaattgc tgacggccat tcaaaaaaat gaaatcaccc
     1321 gcctcggctc gaataaaccg atcgcggtca atgcgcgcct gatctgtgcg accaatatgc
     1381 cgatcaaaca gatggcggag gaaaaaactt ttcgccagga tttattgtac cgcatcaata
     1441 cgatcgaaat ccagttgccg ccgctgcgcg aacggaaacc ggatattcat ttattgacgg
     1501 atcattttgt caaactctat gctaaaaaat atcagaaacc cgcgcacaca ctaagcccgt
     1561 cggctatcca taaactggaa aattacccgt ggccgggtaa tgtgcgcgaa ctgcagcaca
     1621 ccatcgagcg tgccgtgatt ttatgcgatg cccagcttat gaccgcggat gattttgcgt
     1681 tggagttagg tcgcacgcgt acggaaccct tacaagtcga tgattataat ctcgaagaaa
     1741 tcgaaaaagc gctgattcgc aaagtgatcg aaaaacacaa cggcaatatc agccaggccg
     1801 ccaaagaact ggggcttaca cgcacgtcgc tttaccgccg tttggagaaa catggtatct
     1861 aaatttcgac tcatggtgat cgtgcgtatc ctgttattga cagcgacgat caatgtgtgg
     1921 atttatgtca tgcgggcgac ggagctgtat gtcaccatgg cgctgctcgc cgtgatcgtg
     1981 gtgtaccaga tcggcagtat gatcgtgtat gtcgaaaaaa ccaatcgcga cctccggcgt
     2041 tttctggaag ccgttcgtca tgaggatttt acacagacgt ttgccggcgc cggtctcggt
     2101 tcgtcgtttg atgaactgaa atcggcgttt aacgaaatta ttaccaattt ccgcaagaca
     2161 cgcgctgaaa aagaagaaca tttccgctat ctgcaaacgg tcgtccagca tatcggcgtg
     2221 ggtttgatcg cgtttacctc cgacggtacg gtcgagctga tgaatacggc ggcgcgccgg
     2281 cttctcagaa cggggcaatg gcttccgacc gtggatctga aaaatatcca cagtatcaaa
     2341 acattgagcc cgtcgctggc cgatgcgttt atcaacctga aaacagggga taaagccatc
     2401 atcaaagtgg aaatgcggag cgaatttatg cagctgatgg tgcaagccac ggagtttcga
     2461 ttgcgcgagc agctctatcg ccttgtttcc ctccaaaata tccagacgga attggaagaa
     2521 aaagaaatgg aagcgtggca aaatctgatt cgcgtactga cgcatgagat tatgaattcg
     2581 gtaacgccga tttcttcgct cgcatccacc gtcaatgatc tcataaccaa aacttatgaa
     2641 aatgaaaacc aaaacaagcc ggaccccgac acgctttcgg atattcacgg cgctttgacg
     2701 acgatccaaa accgcagcca gggactgatc cattttgtgg atgcgtaccg caatctgacc
     2761 aaaattccaa aacctacgtt tcgcttagtg acggtggagg aactttttgc ccggatcgaa
     2821 catctgatgc gcccgcgcat ggcggagaaa aaaattcttt tcagtaaatc catagacccc
     2881 caatcgctcg agatcaccgc cgacagcatc ctgatcgagc aggtactcat caatctgctg
     2941 gtcaatgcga tgcatgccgt cgaaggccaa tcgcgtccgg aaatctggct tcgtgcggat
     3001 atggacgaaa acgggcgcgt gtgcattcac gtcaccgata atggttacgg tattatcgaa
     3061 gaagcgctca ataaaatctt tattccgttt tttacgacca aacccgacgg cagcggcata
     3121 ggactcagtc tttcgcgtca gattatgcgt ctgcatcgcg gctccatcac cgtccagtcc
     3181 aagccgcatg tgaaaaccgt ttttacatta cgtttttgat cgtccnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnntatga atcatacaat cgtccgattt cttttacttg
     3361 tactgctgct caatgtcgtc cggtatatcg ccgcgtttcc tctggagatg atgctcgttt
     3421 ttccgggact ttttggtgcg atggagtccg cgccgggtgt gtttaatgtg agttttacta
     3481 ccggagactg gatcacgtcg tatgtgtata attttatcct ctggacggta agtgtgttta
     3541 tatttcatcg catggccccg gtcgtcggcg gcaaatggat catcaaaagc gtaaaaatat
     3601 tttttctgcc gtggttgtta tttgtaagcg tcagtgcgat ctatatgaat cactacgcac
     3661 acactaaaat gttttacgtc tatactatgt tagatagtgt gattacgttt ggtatcgtcg
     3721 ccgtggccaa tggtttattg tacccgcgca ttatgggaaa atcttcatcc gtcggataac
     3781 cccaatgcac gttgaccggt gtggtatagg ggcaatcaat tttgatgatg aatgaaagct
     3841 gcgataggcg tttcgtgtct cttttttata tcatattgtc gttgacaata tggaatggat
     3901 aaatatacat tttgtccatg tagccacaaa agcatttcag atcacatagg ggtttttctt
     3961 tatgtttaca tttttcatac taggtattac aaaatgaaac ataaaataat tcgcatagag
     4021 accgttaccc gcaacagcga aaatcatgtt ttgatcagat ttggttttga cgaggaactg
     4081 ataactcttg taaaacaact tccgggcatc cggtggagcg ccgccgataa agcgtggcaa
     4141 atgcctaata cgaaagaaaa cgttaatcgc atttttttac tttttagggg aaaagcgtgg
     4201 gttgattaca atgcattaaa atcccaaaaa ccggaaaata caccggcgcg aaaagacaca
     4261 aacgccggaa tgattttacg gaaacagtta aatgaaagtg tcctaaagaa aattgatcaa
     4321 tttgtaatgt ggctgcagtc caagcggtac ggtgaaaata cgatcaaaac ctacaccgat
     4381 gcgttacgaa catttttgcg gtttcatcac ggcatacctc tcaatgagat taccaatgat
     4441 cacattatta
//
LOCUS       DAOQNQ010000266         4437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_8621_length_4437_cov_5.568005, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000266 DAOQNQ010000000
VERSION     DAOQNQ010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8621_length_4437_cov_5.568005"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PK754_13080"
     CDS             <1..918
                     /locus_tag="PK754_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase subunit L"
                     /protein_id="HNB10483.1"
                     /translation="DEYNDLIVKALAASPISEVLVEESVIGWKEYELEVMRDKNDNVV
                     IVCSIENFDPMGVHTGDSITVAPAQTLTDKEYQKMRDAALKIIRKIGVETGGSNIQFA
                     INPANGDMIVIEMNPRVSRSSALASKATGFPIAKIAAKLAIGYTLDEIPNDITKKTPA
                     SFEPTIDYVVTKIPRWDFEKFQGANTTLNVQMKSVGEAMAIGRNFKESLQKALRSLEN
                     GRFGLGADGKDNPPADASDETAVEAYRQEVINKLRVPNSQRMFAIRDGFKMGMTVEDI
                     HRYTFIDPWFLNNIREIIELEGSLRAALA"
     gene            996..4385
                     /locus_tag="PK754_13085"
     CDS             996..4385
                     /locus_tag="PK754_13085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB10484.1"
                     /translation="MKALALIAWLFVLSFPVFGQIEISFNHVTTKHGLSEGSVHTILQ
                     DRRGFLWFGTQDGLNRYDGYTFAIYRQGSPRQALSDNFIYTLCEDTANGIWIGTFNQG
                     LCRFDRMRESFTIYKHDSTDATSLGDNTIRALYRDRKGILWIGTFRGGLDQMTADGKF
                     KHYRHNPHDPAGLSSDNVWALYEDSRGNFWVGTDGGGLNLLDPMTGKCTVFKNEPGNI
                     RSLSHNTVRAIYEDRAGVLWVATSGGGLNRFDYVTRTFTTFKNHRNDPQSLSHDFVRA
                     LYEDKRGNFWVGTLNGGLNLMDRVTGKFIRFLPDVTDPGSIIHKSVISIVEDRSGTVW
                     FGTWGGGVSYISPTGHRFINYRYDPSNSYSLSQDRIYALHEDQSRTIWVGTFGGGLNR
                     YLPDQKKFLHFQKPQHGLSHDDIWSIAEDQNGDLWVGTNEGGLNRWDRKRGTFEIFRN
                     DPHDANSLSNDNVFTVYVDPLNTVWIGTSGGGLNRFDRNARTFRRFTHDPALGYTVSS
                     NQIRALHQDKEGLLWVGTFGGGISVWDRKKEIFTRHLSSNYDSSSLSSDLVLRIFESR
                     DGLIWIGTAGGGLNRYDKQTGHWKTYTDKHGLANNVINGILEDQTGRLWISTNKGLSR
                     LDPKTETFRNFDEGDGLISAEFGVGAALRAHDGRMYFGSNRGLVTFHPDSIKENRYVP
                     PVIIAGFKKFNKPFVLERSVSETDTIIISYKDNFIAFEFAALNYTHSEKNLYRYKLEG
                     FDRDWIDAGSRREATYTNLDGGTYVFRVKASNNDNVWNEEGRAITLIVEPPFWETVWF
                     QFLVGAFLCAVLVYRFRQIQNQKRELERQVQVRTHELEKAMDSLKRAQVQILQSEKIG
                     SLAHMVAGIAHEINNPLTFVYSNIQLLRARVQRLFLFMGFLDKRSHTESWTDDEKKQY
                     EKFKHTLDYDYLEKDLDDMFRSSVEGSTRIIKIVNSLRAFSSLDQEEIQETNLLESLN
                     MVVDLFMRQHKDIVIERKTTEIPFITVHLTLMHQAFINILDNSVKAIRDAEKQNLLAS
                     GDGRITLSAEWQSDKNKIRLCFIDNGVGIDSSVRNKIFDPFFTTRPIGAGAGLGLSEV
                     FTTVRKHGGDVTVESEPGIGTAIHIDLPVKTLFHDDYKHGS"
BASE COUNT         1230 a          923 c         1084 g         1200 t
ORIGIN      
        1 gacgaatata acgatctgat tgtcaaagct ttggcagcga gtccgatcag tgaagtgctc
       61 gtcgaagaat ccgtgatcgg gtggaaagaa tatgaactgg aagtaatgcg cgataaaaac
      121 gataacgtcg tcattgtctg ttcgatcgaa aatttcgatc cgatgggcgt gcacacaggc
      181 gactcgatca ccgtcgcacc ggcgcaaaca ctgacggata aagaatatca gaaaatgcgc
      241 gatgccgcat tgaagattat tcgtaaaatc ggtgttgaaa ccggaggttc caatattcag
      301 tttgccatta atcccgctaa cggcgacatg atcgtgatcg agatgaatcc gcgtgtgtcg
      361 cgcagttcgg cgttagcctc caaagcaaca ggatttccga ttgcaaaaat cgccgctaaa
      421 cttgctatcg gatacacact agatgagata ccgaacgata ttacgaaaaa aaccccggca
      481 tcgtttgaac cgacgattga ttatgtagtt accaaaattc cgcgttggga tttcgaaaaa
      541 tttcaaggcg ccaatacaac gctcaatgtg cagatgaaat cggtcggtga ggcgatggcc
      601 attggccgta actttaaaga gtcattacaa aaagctttgc gttcgctcga aaacgggcga
      661 tttggacttg gcgcagatgg taaagacaat ccgccggcag atgcgtcaga tgaaacggcc
      721 gtcgaagcgt atcgtcagga agttatcaat aaactccgcg tacccaattc acaacggatg
      781 ttcgccatac gtgacggttt caagatgggt atgacggtag aagatattca ccgctacaca
      841 tttatagacc cctggtttct caataatatc cgtgaaataa tcgaattgga aggatcgctc
      901 cgcgcggcgt tggcttgatt ggtaaacaat acaaggcttg atatccgtta agcgtttcgc
      961 tcccctctgt tatttcctat cgaatcaata attccatgaa agcacttgcg ctcatagctt
     1021 ggctgtttgt tctatccttt cccgtttttg gtcaaatcga aatttcattt aatcacgtca
     1081 caactaaaca tggtctttcc gagggatcgg tgcatactat attacaagac cggcgcggtt
     1141 ttttgtggtt cggtacgcaa gacggtctta atcgttatga tggatatacg tttgctatat
     1201 acaggcaagg ttctccgcgt caagcgctaa gcgataattt tatatatacg ctgtgcgaag
     1261 atacggcaaa cggcatatgg atcggtactt tcaatcaagg gctttgccgc tttgatcgta
     1321 tgcgcgaatc atttacgatt tacaaacatg attctacgga tgccacttcg ctcggagata
     1381 ataccatacg tgcgctgtat cgtgatcgca aaggaatttt gtggatcggc acatttcgcg
     1441 gcggcttgga tcaaatgacg gcggatggga aattcaaaca ttaccgtcac aatccccatg
     1501 atccggccgg tttgtcgtct gacaacgtgt gggcattgta tgaagatagc cgcggtaatt
     1561 tttgggtagg gacggatggc ggtggtctta atttgttgga tccgatgacg ggaaaatgta
     1621 ccgttttcaa aaatgaaccg ggtaatattc gatcccttag tcacaacacg gtgcgtgcga
     1681 tttacgaaga tcgtgccggt gtcttatggg tggctacatc cggagggggg ctcaatcgtt
     1741 ttgattatgt tacgcgtact tttacaacat tcaaaaatca tcggaacgat cctcagagtt
     1801 tgagtcatga ttttgtacgg gctttgtatg aagacaaacg tggaaatttt tgggtaggca
     1861 cgcttaacgg agggttaaat ttaatggatc gtgttaccgg gaagtttata cgatttcttc
     1921 ctgatgttac cgatccgggt agtataattc ataaaagcgt aatatctata gtggaagatc
     1981 gatccggcac ggtgtggttt ggtacatggg gcggcggtgt aagctatatc agtcccacgg
     2041 gacatcgatt tatcaactat cgttatgacc cgtccaattc gtactccctg agtcaagatc
     2101 gcatttatgc gctgcatgaa gatcagtccc gcactatttg ggtcggaaca ttcggcggtg
     2161 gtctgaaccg gtatttgccg gatcagaaaa aatttcttca ttttcaaaaa ccccaacacg
     2221 gtctcagtca tgatgatata tggagtatag ccgaagacca aaacggtgat ctttgggtcg
     2281 gtaccaatga aggagggctc aatcgttggg accgaaaaag aggaacattt gaaatttttc
     2341 gcaatgatcc gcatgatgcg aattcgctta gcaacgataa tgtttttaca gtttatgtgg
     2401 atccgttaaa taccgtttgg attggaacct ccggcggcgg tcttaatcgt tttgatcgta
     2461 atgcgcgtac gtttcgtcgt tttacgcatg atccggcgct tggatatacg gtcagtagca
     2521 atcaaattcg cgcgttgcac caggataaag aaggattgtt gtgggtcggc acgttcggcg
     2581 ggggcatcag tgtgtgggat cgaaaaaaag aaatatttac ccggcattta agttcaaatt
     2641 acgattcgtc gtcgctctca agcgatcttg ttttacgtat ttttgaaagt cgtgacggtc
     2701 tgatatggat cgggacagcc ggcggcggtc ttaatcgtta cgataaacag acgggtcatt
     2761 ggaaaacata taccgataaa catggtttag cgaacaacgt cattaacggt atcttggaag
     2821 accaaaccgg ccgcctttgg atcagtacca ataaaggcct gagccgatta gaccctaaaa
     2881 cggaaacatt tcgcaatttt gatgagggcg acggcttgat ctctgcggaa ttcggtgtcg
     2941 gtgcggcctt acgcgcccat gacggccgta tgtattttgg aagtaacaga gggctggtga
     3001 cttttcatcc ggacagtatc aaagaaaacc gttatgtacc tccggtaata atcgcgggtt
     3061 tcaaaaaatt taacaaaccc ttcgtcctcg aacgatccgt ttctgaaacg gacacgatca
     3121 ttatttcata caaagataat ttcatcgcgt tcgaatttgc ggcactcaac tatacccata
     3181 gcgaaaaaaa tttataccgc tacaaattag aaggttttga tcgcgactgg atagacgccg
     3241 gtagccgccg cgaagcgacg tataccaatc tggacggcgg cacgtacgta ttcagggtca
     3301 aagccagtaa taatgataat gtgtggaatg aggaagggcg tgcgatcacc ttgattgtag
     3361 aaccgccgtt ttgggaaaca gtgtggtttc aattcctggt aggtgccttt ttatgcgcgg
     3421 ttcttgtgta tcgcttcagg caaatacaga atcaaaaaag agaattagaa cgtcaagtcc
     3481 aggtgcggac acacgaatta gagaaagcga tggattcgct taaacgcgct caggttcaga
     3541 ttcttcaatc cgaaaaaatc ggttcattgg cacatatggt ggccggcatt gcgcacgaga
     3601 tcaataatcc tctgacgttt gtgtattcca atattcaact tcttcgcgcg cgggttcaac
     3661 gattatttct tttcatgggg tttcttgata aacgctcaca cacggaatca tggacagatg
     3721 atgaaaaaaa acagtatgaa aaattcaaac ataccttgga ttacgactac ctcgaaaaag
     3781 acctggatga catgtttcga agttctgtag aaggtagtac ccgtattatc aaaatcgtaa
     3841 atagcctgcg agcgtttagc agtctggatc aggaagaaat acaagaaacc aatttgctgg
     3901 agtcgctcaa tatggtggtg gacctgttta tgcgccagca taaagatatt gttattgaac
     3961 gcaaaaccac cgaaattccc tttataaccg ttcacttgac gcttatgcat caggctttca
     4021 ttaatatcct cgataattcg gtcaaagcaa tccgcgatgc ggaaaaacaa aatttgctgg
     4081 caagcggaga tggtcgtatt acattatctg ccgaatggca atcggataag aataaaattc
     4141 gcctttgctt cattgataac ggtgtgggca ttgattcatc tgttcgaaat aaaatttttg
     4201 atccgttttt tacaacgcga cccatcgggg ccggcgcagg actgggtttg tcggaagttt
     4261 ttacgacggt gcgaaaacat ggcggcgatg tcactgtcga aagtgagccc ggtattggca
     4321 ctgcgataca catagacctt ccggtaaaaa cattatttca cgatgactat aaacacggct
     4381 cgtgatgaaa actaaaataa tgtatggctt gttttgtgtg atttggcttt cgcccct
//
LOCUS       DAOQNQ010000267         4429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_8654_length_4429_cov_5.217421, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000267 DAOQNQ010000000
VERSION     DAOQNQ010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8654_length_4429_cov_5.217421"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /locus_tag="PK754_13090"
     CDS             <1..1240
                     /locus_tag="PK754_13090"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNB10485.1"
                     /translation="VQTDTLTVRDFERIFWIENDIVTLRIKGEDILKIIKRSDESRTK
                     YNDRFVHLKAVNSTGKTDGFNVHGRAVLADQFYTVVTSNFLANGGDGYNEFLRGTRKQ
                     EHFALTKDGWRQQVEGKLLPIRKTFIEYLIENKDRNIPRRFDHPVWFMTMRKIGFQAE
                     NKTKKNDALYKDYSINELQGEEKVAVSSTLDMKLTRWTRDLIIENYIKSEYGRIKLGN
                     DPYRESDDNWSVESLVALPLIEQPIVSSVAVYPYAASKFQSEFTADQGPRKKDLYIHT
                     GAAFSDFWNFSENRVGLVHLTRIEGNERRIGNFGVGVHSLFSNTRGNYGNFSEFEFIY
                     FFTKAKKKKSVAEADSASSNTRSLRFNWSSLIAIERFKNFYFNPGVNFFAYKGYGRDK
                     WAYSVALQFGLVFTAGWKFW"
     gene            complement(1275..1357)
                     /locus_tag="PK754_13095"
     tRNA            complement(1275..1357)
                     /locus_tag="PK754_13095"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1322..1324),aa:Leu,seq:cag)
     gene            1523..3526
                     /locus_tag="PK754_13100"
     CDS             1523..3526
                     /locus_tag="PK754_13100"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNB10486.1"
                     /translation="MRKQLLTLLFFLVSGVELVVAQERIFEKPLSPRIANYDLRAELK
                     PELKTVEASWNLHWWNASRDTVKELQFHLYLNAFRNERSTFIRESGGRHRSGEINLDS
                     ANWGYILIKNLAMAGGENLTTRIEYIAPDDGNKDDRTVIRVPLSRPIPPGGDIRLTCD
                     FTSKLPRVYARTGFTEDDFYLIGQWFPKIGVWENGAWNCHQFHSNSEFYADFGVYTME
                     LTVPKKYVVGANAVLMEKKALNDSTNLFRYRQEDVIDAVWTAYPHFKELQQTVTIKET
                     GRQVVVTYLVPPERLWVMPKYYATSEAMFNYYQEWYGAYPYPNLTIVDMPVGEEMTAG
                     GMEYPTLVTTGSYFGWPFINKYIVPNHWLETVTFHEFGHNYFQSLVATNEFEHPWMDE
                     GFTQYTEARASVRFAKENNIAYEMADIFGIPFNVLAYNRSSYTSRPKDGVIEAFSWNI
                     PESFYQPAAYAKPALVLETLQRHLGDAMWNKVMRTYFERYSFKHPKPRDFYAVVNEVT
                     GKDFMWYFDQFVNKPVTVDYQIAGVFNEEIHASRGWFGDGNNLSFTGSDASDSLKQYR
                     SGFSVRNMGDGYFPTKILVCFADGDSIVETWDGQYAPQEFVYHGKAKIKSVHIDPDRV
                     NLLDINWTNNSRVLERDEVGIQRYITRFLFWLQNIGQVLAVLV"
     gene            3539..4396
                     /locus_tag="PK754_13105"
     CDS             3539..4396
                     /locus_tag="PK754_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10487.1"
                     /translation="MKLTHIYFSAWHATWNNRRMAIMLWFTTLITAIPGYVMAYRTMG
                     QYFGRTEVPSLWKNDFELNYLFDLLIDQPSAGTIISSVFGGAAIVSLILMIFLTGGIV
                     AALIHPNEQNHFWHNCGKYFGRFFRLFLWNAVFVMIGIALIPTHEYGWIITVILSGTA
                     IMAGDIAKIRIVYNAHYSVTRMYFQSWRWVWQHVHHISELYIANVIAFVAVFALYKYL
                     DNGFVANSGLKLLMMIVLHQLFVCFRAMMRIQFLAAPAELWKNLHYTVVPPRLKKTNI
                     LVAAPEYSN"
BASE COUNT         1186 a          929 c         1090 g         1224 t
ORIGIN      
        1 cgtacaaacg gacaccctga cggtacggga ttttgagcgt atattttgga tcgaaaacga
       61 tattgtcaca ctccgcatca aaggcgagga tattctcaaa atcatcaaac ggagtgatga
      121 gagccgcacc aaatacaacg atcgttttgt tcatctcaaa gcggttaatt cgacgggcaa
      181 aacggatggc ttcaatgtgc acgggcgtgc ggtgttagct gatcaatttt acacggtggt
      241 cacttccaat tttttggcca acggcggcga cgggtataac gaatttttac gcggcacgcg
      301 taaacaggaa cattttgcgc tcactaaaga cggatggcgt caacaggtcg aaggaaaatt
      361 actgccgata cgaaagacat ttatcgaata cttgattgag aataaagacc ggaatatacc
      421 acgaaggttt gatcatccgg tgtggtttat gacgatgcgc aagatcggtt ttcaagcgga
      481 gaataaaaca aaaaagaatg acgctctgta taaagactac tccatcaacg aattgcaagg
      541 cgaagaaaaa gtagcggttt cctccacgct tgatatgaaa ctgacccgct ggacgcgcga
      601 cctcattata gaaaactata ttaaatcgga gtacggtcgc atcaagttgg gcaatgaccc
      661 ttaccgagaa tcggatgata actggtccgt cgaaagtctc gtggctttgc cgctcatcga
      721 acagcccatc gtaagttcgg tagccgtata tccttatgcg gcgtctaaat ttcaatccga
      781 atttacggcc gatcagggcc cgcgaaaaaa agatttatac attcataccg gggcggcgtt
      841 ttcggatttt tggaattttt ccgaaaaccg cgtcgggctc gtccatctaa cccggatcga
      901 gggtaatgaa cgacgaatcg gcaattttgg tgtcggtgtg cattcgctat tttccaatac
      961 acgcgggaac tatggtaatt tttctgaatt tgaattcatc tattttttta ccaaagccaa
     1021 aaagaaaaaa agtgtggccg aagccgacag cgcgtcatcc aatacgcgtt ctttgcggtt
     1081 caactggtca tctcttatcg ccatagagcg gtttaaaaat ttttatttta atcccggcgt
     1141 gaattttttt gcttataaag gatacgggcg ggacaaatgg gcgtattcgg tggcgttgca
     1201 atttggtctt gtatttacgg caggttggaa attttggtaa taaaaaaatc ccgcaatggg
     1261 tttgcgggat tgggtgccga aggggggagt tgaaccccca cgcccttgcg ggcactagta
     1321 cctgaaacta gcgtgtctgc catttcacca cctcggcaag gcgggcaata ataagataat
     1381 ttatttgaaa cgtcaaataa attttagata gagcttgaac aacgcaacat tttgaccgta
     1441 tagcggtcta acggactagt gatgtttcta gttctaaatt tgtttactaa tgtacttttt
     1501 aaatccatcg aggaattggg agatgagaaa acagttacta actttacttt tctttctggt
     1561 gtcaggagtc gaattggtcg tggctcagga acggattttt gaaaaacctt tgagcccgcg
     1621 tattgccaac tatgatttac gggcggaatt gaagccggaa ctgaaaaccg tcgaggcatc
     1681 gtggaatctg cattggtgga atgccagtcg tgatacggta aaggaattgc aatttcacct
     1741 ttacctcaat gcatttcgca acgaaagatc tacttttatt cgtgaaagcg gaggacggca
     1801 tcgttccggt gagattaatc tggattcggc caattgggga tatatcctca taaaaaactt
     1861 ggcgatggcc ggcggagaaa acctcacgac gcgtatcgaa tacatcgcac cggacgacgg
     1921 aaataaggac gatcgtacgg taatacgtgt tccgctgagt cgtccgatac ctccgggcgg
     1981 agatattcgg cttacctgcg atttcacatc caaactgccg cgtgtgtatg cacgtacggg
     2041 ttttacggaa gatgattttt atctcatcgg tcaatggttt ccaaaaatcg gagtatggga
     2101 aaacggagcg tggaattgcc atcagtttca ttccaattct gaattctacg cggacttcgg
     2161 cgtttatacg atggagctaa ccgttcctaa aaaatacgtc gtcggcgcca atgccgtgct
     2221 tatggaaaag aaagcgctca acgacagtac caatctattc cgctatcgtc aggaagatgt
     2281 aattgatgcc gtctggacag cgtatcctca ctttaaggaa cttcagcaaa cagtgacgat
     2341 caaagaaaca gggcggcagg tcgtcgtgac gtatttagtt cctccggagc gtctttgggt
     2401 aatgcccaaa tattatgcaa catccgaagc gatgttcaat tattatcaag agtggtacgg
     2461 tgcgtatccc tatcccaatc tgacgatcgt agatatgcct gtaggtgaag aaatgaccgc
     2521 cggcggtatg gagtatccga cacttgtcac gacgggttct tatttcggat ggccttttat
     2581 caataaatat atcgtgccca accattggct ggagaccgtg acgtttcatg aattcggcca
     2641 caattatttt caaagcctcg tagcgaccaa tgaatttgaa catccgtgga tggatgaggg
     2701 atttacgcaa tataccgaag cgcgtgcgtc cgttcgtttt gcaaaggaga ataatattgc
     2761 ttacgaaatg gcggatattt tcggtattcc tttcaacgta ttggcctata atcggagcag
     2821 ctatacgagc cgacccaaag acggtgtgat cgaagctttt tcgtggaata ttccggaatc
     2881 tttttatcag cctgcggcct atgccaaacc ggcgcttgtt ttggaaacac tgcaacgcca
     2941 cctcggtgat gcgatgtgga ataaagtcat gcgcacgtat tttgaacgtt acagtttcaa
     3001 gcatcccaaa ccgcgcgatt tttatgcggt ggtcaatgaa gtgaccggga aggattttat
     3061 gtggtatttt gatcagtttg tcaataaacc ggtaaccgta gattaccaga tagctggggt
     3121 atttaatgaa gaaatacatg cgtcccgggg ctggtttggc gatggtaaca atctgagttt
     3181 taccggcagc gatgcatcgg attcactaaa acaataccga agcggatttt ccgtacgcaa
     3241 tatgggcgac ggttattttc cgacgaaaat tttggtttgt tttgccgacg gcgattccat
     3301 tgtcgagaca tgggacggtc aatacgcgcc ccaggaattt gtttaccatg gtaaggcgaa
     3361 aatcaaatcg gtgcatattg atccggatcg cgtcaattta ttggatatca actggaccaa
     3421 taatagccgc gtactcgagc gggacgaagt cggaattcag cggtatatta cgcgttttct
     3481 tttttggtta caaaatatcg gccaggtgct ggccgtattg gtttaaggag aatacacaat
     3541 gaaacttaca catatctatt tcagcgcttg gcatgcaact tggaacaacc gccgtatggc
     3601 gatcatgctg tggtttacta ctttgatcac ggcgataccc ggatacgtta tggcgtaccg
     3661 cacgatgggg cagtacttcg gacgcacaga ggtaccctcg ctgtggaaaa atgattttga
     3721 acttaactac ttatttgact tgctgattga tcagccttcg gcgggtacga ttatttcgtc
     3781 ggtctttggc ggagcggcga tcgtctcact tatactcatg atttttttga caggcggcat
     3841 tgttgcggcg cttatccatc ccaacgaaca aaatcatttt tggcataatt gcggtaaata
     3901 cttcggtcgt tttttccggt tattcttgtg gaatgcggta tttgttatga tcggcatagc
     3961 gctcattccc acgcatgaat acggctggat tattacggtg atactctccg gtacggcgat
     4021 catggcggga gatattgcta aaattcgtat cgtctataat gcacactatt ccgtaacgcg
     4081 gatgtatttt cagtcatgga gatgggtttg gcagcatgtg catcacatca gcgaactgta
     4141 tatcgccaat gtgatcgcgt ttgtggccgt tttcgcactg tataaatatt tggacaacgg
     4201 attcgtggcg aatagcggac taaaactttt gatgatgatc gtcttacatc agctatttgt
     4261 atgtttccgc gcgatgatgc ggattcagtt tttggctgca ccggcagaac tgtggaaaaa
     4321 tctgcattat accgtcgtgc cgccgcggtt aaaaaaaaca aatattctgg tcgctgctcc
     4381 ggaatattcc aattaaaaac tcagaggcta tgatatggga caagatgtt
//
LOCUS       DAOQNQ010000268         4422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_8685_length_4422_cov_5.604763, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000268 DAOQNQ010000000
VERSION     DAOQNQ010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8685_length_4422_cov_5.604763"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1782
                     /locus_tag="PK754_13110"
     CDS             <1..1782
                     /locus_tag="PK754_13110"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HNB10488.1"
                     /translation="HLAVSVTMAMPCGVPTKPDCPSCACANASGAAPHSTAPTASAPS
                     QLRYLIPFEAPEKTIDYLRDLAEKNENAVAVFADDGEKFGIWPDTYQHVYENGWLQRF
                     FEVLEANRDWIEICHFSDVLRSVPPKGKIYLPTASYSEMLHWALPLSGYRNYELFQSK
                     LKEEKIAEPYNAFVRGGFWRNFFSKYAESNRMHKKMLRVSARLAKVAEVIPKDDKVKQ
                     SLLESAQNHLWASQCNCPYWHGVFGGIYLNNIRFAVYRHMLNAEVLTDQLDAYTQGSA
                     YRLEETDFDTNGKNEILFESPKLNAYLDPQHGATLFEIDYKEIPMHVTDILTRREEGY
                     HHQLAEASITEPSKKNTESDDSIASIHDRVLSKEPDLHRHLIYDQYERRSFRDHVLTD
                     NCDLEAWQNQTYSVYADLADASYDVERKEYERYVEITCLRETELEMAPMRIEKKYKIF
                     SDDPRIEVHYRWHNRGKTEVKAKFGTEINLTLLAGQADDRFYAVGGRKWPDGAMQSSG
                     TFAGIRSLNMIDGWQNLNIALAWSETADVWRYPIETVSLSEEGFERVYQGSCVFVHWP
                     MWVKPNDEWNVSIVLHFSRDTRKFFQS"
     gene            1796..3367
                     /locus_tag="PK754_13115"
     CDS             1796..3367
                     /locus_tag="PK754_13115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNB10489.1"
                     /translation="MRKKWLIAVGVGIAAIGILGFTGPGQNAYKSIQKLQYVLSIVTQ
                     TYVEDVNSDKLVEDAIVGALKGLDPHSIYIPPKEMKTVKEEFQGNFDGIGSQLDVING
                     IPTIVTPIPGSPSEKVGLLAGDKILKINDEPTKGFTIDQAVSRLRGPKGTKVRIAVQR
                     TGVNEMLDFEIVRDKIPVYSVDAKFMIDEKTGYIRFVRFAETTEEEIEQALKELEAQG
                     MQQLVLDVRNNGGGYLEEAQRIVDKFIPGGKKIVYTKGRIANASRDYFSSDKTTYRKY
                     PIIILVNRGSASASEIVAGAIQDLDRGIVVGERTFGKGLVQNQFDLNDGSSVRVTTAR
                     YYTPSGRLIQRPYDGKSLDQYYGEVRRDTVHSDTTHIYYTASGRKVFGGGGIVPDYHI
                     DNDTVSAYYVKLWGRGIFRDFTNDYLEKNGPKLRDQYKDQFKKFTAGFQISDEDFDAL
                     IKMGESRGIAKEADAIVRDKEDMSNFVKAEVARFIWGSYAAAQVRSMTDSAILKTAKY
                     FGEAKKFADQNAKLK"
     gene            3387..>4422
                     /locus_tag="PK754_13120"
     CDS             3387..>4422
                     /locus_tag="PK754_13120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNB10490.1"
                     /translation="MKKKIILPVLLVLVIIVAVTGFTSTGDVYKNLVKLKTIMDLVEQ
                     TYVEDVNGDKMVDDAIAGALKGLDPHTVYIPPKEMKTVKEEFQGNFEGIGISFEIVDG
                     NLTVVTPIPGTPSEKAGLLAGDRIVRIDGDLTKGITNDGVFSKLRGPKGSKVHITIER
                     PGVSEQIEFDIIRDKIPLYSVDAKFMLDDKTGFIHFSKFSETTEEEIEQALRELEAKG
                     MQQLILDLRNNGGGYLEQAQMVVDKFIPGGKKIVYTKGRIPNSTKDYVSSSKSDYRKM
                     PMIILINRGSASASEIVSGALQDLDRALIVGERSFGKGLVQSQYELGDGSALRVTTAR
                     YYTPSGRLIQR"
BASE COUNT         1256 a          969 c         1133 g         1064 t
ORIGIN      
        1 cacttggccg tgtcggtcac gatggcgatg ccctgcggcg tgcccaccaa accggattgc
       61 ccaagctgcg cctgtgcgaa cgcgtccggg gccgcgcccc acagcaccgc acccaccgcg
      121 agcgcgccca gccagttgcg gtatctcatc ccgttcgaag cgccggaaaa aacgatagat
      181 tacctgcgcg atcttgcgga aaaaaatgag aacgccgtag ccgtatttgc cgacgatggc
      241 gaaaagtttg gcatatggcc ggacacatat cagcatgtgt atgaaaatgg gtggcttcaa
      301 cgtttttttg aagtacttga ggcgaaccgg gattggatcg aaatatgcca tttttccgat
      361 gtcctgcgat cggtcccgcc caaaggaaaa atttaccttc cgacagcttc gtatagtgaa
      421 atgttgcact gggcattgcc gttaagcggt tatcgtaact atgaattgtt tcagtcgaaa
      481 ctgaaagaag agaagatcgc ggagccttac aacgcgtttg tgcgtggcgg tttttggcgt
      541 aattttttta gtaagtacgc cgagagcaac cgtatgcata aaaaaatgtt acgtgtcagt
      601 gcgcggttgg ccaaagtagc tgaagttatt ccaaaagacg ataaggtaaa acaatcgctg
      661 ctggaatccg cccaaaacca cctgtgggct tcgcaatgca attgccctta ctggcacggt
      721 gtattcggag ggatatacct caataatatt cgttttgcgg tgtaccggca tatgctgaac
      781 gccgaagtac taaccgatca gctggacgca tatacccaag gatccgctta tcgtttagaa
      841 gaaacggatt ttgatacgaa cgggaaaaat gaaatccttt ttgaatcgcc gaagctaaat
      901 gcctaccttg atccgcaaca tggtgcgacg ctttttgaga ttgattataa agagattccc
      961 atgcacgtga cggacatttt gacccggcgg gaagaaggat atcatcatca gttggcggag
     1021 gcttccatca ccgagccatc taaaaaaaat accgaatcgg acgacagcat tgccagcatc
     1081 catgatcgtg tattatccaa agaaccggat ttacataggc atctgatcta tgatcaatac
     1141 gagcgccgtt cgtttagaga tcatgtgttg acggacaatt gtgatttgga ggcatggcaa
     1201 aatcagacct acagcgtata tgccgatctg gccgatgcat cgtatgatgt ggaacgaaaa
     1261 gaatacgaac gttatgtgga aatcacatgc ctgcgcgaaa cggaactaga gatggcgccg
     1321 atgcgaatcg aaaaaaaata taaaatattc agcgatgatc cgcgcattga agtacattac
     1381 cgctggcaca atagaggcaa aaccgaggtt aaagccaaat ttggaacgga gatcaatctg
     1441 acacttttgg ccgggcaagc ggatgatcgt ttttacgcgg tcggcggcag aaaatggccg
     1501 gacggtgcga tgcagtcgtc gggaacattt gccggtattc ggagtttaaa tatgatagac
     1561 ggttggcaaa atctgaatat cgcactcgct tggtctgaaa cggccgatgt ttggcgatat
     1621 cccatcgaaa cggtttcgct atccgaagag ggatttgaac gggtttatca ggggtcgtgt
     1681 gtttttgtac attggccgat gtgggtgaaa cctaatgatg aatggaacgt aagtatagta
     1741 ttgcactttt cccgcgacac gcggaaattc tttcaaagtt aatcgggagt ttttcatgcg
     1801 caaaaaatgg ttgattgccg tcggcgtagg tatcgccgcg ataggtatcc tcgggtttac
     1861 cgggcccgga caaaacgcct acaaaagcat tcagaaatta caatatgtac tctcgatcgt
     1921 tacacagacg tacgtcgaag atgtcaattc ggataaactg gtcgaagatg ctattgtcgg
     1981 tgccctcaaa ggacttgatc cgcattcgat ctatatcccg cctaaagaaa tgaaaaccgt
     2041 taaggaagaa tttcagggaa attttgacgg catcggctca cagcttgatg tgatcaatgg
     2101 cattccgacg atcgttacgc cgatacccgg cagcccttct gaaaaagtag gattattggc
     2161 cggcgataaa attttaaaga tcaatgatga acccaccaaa gggtttacca tagaccaggc
     2221 cgtatcaaga ctgcgcggtc ccaaaggaac caaagtgcgt atcgccgtac aacgcaccgg
     2281 ggtcaacgaa atgcttgatt ttgaaatcgt gcgtgacaaa atacccgttt acagcgtgga
     2341 tgcaaagttc atgattgacg aaaaaaccgg atatatacgt tttgtacgtt tcgctgagac
     2401 gacggaagaa gaaatcgagc aagcgcttaa agaactcgaa gcgcaaggca tgcaacagtt
     2461 ggttttggat gtacgcaata acggcggcgg ctatctcgaa gaagcgcaac gcatcgtaga
     2521 taaatttatc cccggcggta aaaaaatagt atataccaaa ggtcgtattg ctaatgcatc
     2581 acgcgattat ttttccagcg ataaaacgac ctatcgtaaa tatccgatca tcattcttgt
     2641 caatcgcggc tcggcctcgg cttcggagat cgtcgccgga gcgatccagg atttggatcg
     2701 cggtatcgtt gtcggtgaac gcaccttcgg caaaggattg gtacaaaatc aatttgattt
     2761 aaatgacggc tcatcggtgc gcgtcacgac ggcccgttac tatacgccga gcggtcgtct
     2821 tattcagcgt ccttacgacg gcaaatcatt agatcagtat tacggcgaag tgcgtcgtga
     2881 tacggtacac tccgatacga cccatattta ttacacggca tccggtcgca aagtattcgg
     2941 cggcggcggt attgtgccgg attatcacat tgataacgat accgtttcgg cctactacgt
     3001 caaactctgg gggcgcggta tttttagaga ttttactaac gactatcttg agaaaaacgg
     3061 acccaaactg cgcgatcagt acaaagatca attcaaaaaa tttaccgcgg gattccagat
     3121 cagcgacgaa gattttgatg cgctgattaa gatgggcgaa tcaagaggga ttgccaaaga
     3181 agccgatgcc atcgtgcgag acaaagagga tatgtccaat tttgttaaag cagaagtagc
     3241 gcgatttata tggggcagtt atgcggcggc ccaggtgcgt tcgatgacgg acagcgctat
     3301 tctcaaaacg gcaaaatatt tcggtgaagc caaaaaattt gccgatcaaa acgctaaatt
     3361 gaaataacgg tcgtaatgga gcatatatga agaagaaaat aattttgccg gtgcttttgg
     3421 tgctggtgat cattgtagcg gtaacgggtt ttacatccac cggcgatgtg tataaaaacc
     3481 tcgtcaaact caagaccatc atggatcttg tggaacagac gtatgtcgaa gacgtcaatg
     3541 gcgataaaat ggtggacgat gcgattgccg gagcgctcaa aggcttggat ccgcacacgg
     3601 tgtacattcc gcccaaagag atgaaaaccg ttaaggaaga atttcaaggt aattttgaag
     3661 gtataggtat ttcgtttgaa atcgtggacg gcaatctgac agtggttacg ccgatacccg
     3721 gtacaccgtc ggaaaaagca ggtcttctgg caggcgatcg tatcgtcaga atagacggcg
     3781 accttaccaa aggtattacg aacgatggcg ttttttcgaa attacgtgga cccaaaggtt
     3841 ccaaagtgca catcacgatc gaacgtcccg gtgtgtctga acaaatcgaa tttgatatca
     3901 ttcgcgataa aataccgctg tatagcgtgg atgcgaaatt catgctggat gataaaaccg
     3961 gttttattca cttcagtaaa ttcagtgaaa caaccgaaga agagatcgaa caagccttgc
     4021 gcgaactcga agccaaagga atgcagcagt tgatcttgga tttgcggaat aacggcggcg
     4081 gctatctcga acaagctcag atggtcgtgg ataaatttat ccccggcggt aaaaaaatcg
     4141 tttacaccaa aggacgtatt cccaattcga ccaaagatta tgtgtccagt tcaaaatcgg
     4201 actatcgcaa aatgccgatg atcattctga tcaatcgtgg ttcggcttcg gcttccgaaa
     4261 ttgtctccgg cgcgcttcag gatttggatc gggcgctgat cgtcggtgaa agaagtttcg
     4321 gaaaagggtt agtgcagtcg cagtacgaac tcggagacgg ttcagctttg cgtgttacga
     4381 cagcgcgtta ttatacgccg agcggtcgtc tgatccagcg cc
//
LOCUS       DAOQNQ010000269         4408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_8715_length_4408_cov_5.895015, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000269 DAOQNQ010000000
VERSION     DAOQNQ010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8715_length_4408_cov_5.895015"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="PK754_13125"
     CDS             <1..981
                     /locus_tag="PK754_13125"
                     /inference="COORDINATES: protein motif:HMM:NF017893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HNB10491.1"
                     /translation="EVDYRQKGLLWYSVYKVRFSGLYTFTNPSAKEEDVTIDFTFPAA
                     QAIYDDFVFQWDGQELLKTETIRESSDDPYRQDSKPGSLRANIKMKPGQIAVLKVGYT
                     SQGTGTWEYSVGEGTKRVTDFQLTAQTHFKEFDIPEFSISPGTKTETADGWSLVWQYK
                     DIISDVRIGLGMPKKLDPGPVAERISFFAPVSLLFFFCILIIIGAIRDVRMHPMNYFF
                     LAAAFFSFHLLFAYLVDHLALELSFAISSIVSVTLVAAYMRLVTSWPYTVRVVMPTQV
                     IYLILFSYSFFFEGYTGLTITIGAIVTLFILMQMTGKTDWNAIFKKDIKS"
     gene            complement(1055..1999)
                     /locus_tag="PK754_13130"
     CDS             complement(1055..1999)
                     /locus_tag="PK754_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10492.1"
                     /translation="MKSFLRLMLAGLILTVTTGRAMAGDDLEAKIKSMASANAQSYIK
                     PIVSGFGVAMNSGIFRSPETHSVLGFDFQLVTAMAFAPSSEKTYTPVVPNGYVPAASN
                     EANTAIGSKDPTTYYLGGIPNDQTNIVLPGGIDAPAGASGFFVAPQLTVGVPFKTDVI
                     LRFVPGIPAGKLGKVKLFGLGAKHNINQWLPISLPLDIAAGAFYQKMSIGDVFEAKAT
                     SFDILAGKSLNILIIKLGLYGGVGIESSSMDINYETTGTASGVPEGTKVKFSVDGKNS
                     MRGIVGANIRLLWLINVNPEMSFGKYRSTSIGVGLTLR"
     gene            complement(2233..2946)
                     /locus_tag="PK754_13135"
     CDS             complement(2233..2946)
                     /locus_tag="PK754_13135"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB10493.1"
                     /translation="MKDVGRDTIKNRSSIIRSFFPGTGHSYDRVVHGFTLGLDYHWKR
                     MLLRHIPKDSRRVLDLACGTGIVTEGIAKRCPDADIVGVDITDDYLKIYERRIRSKNL
                     RASFLLGNAEDVKLDGIFDAVISSYIPKYVDPEILLSNINPHLKSSGVIAVHDFYYPP
                     NSVARAIWRKYNRAMNFVGMRFFPEWHEVFDGGLTSLIQSSNWFEKWQESLIRFEYEI
                     KTAHLLSFGSSAIIVARKK"
     gene            complement(2980..3465)
                     /gene="msrB"
                     /locus_tag="PK754_13140"
     CDS             complement(2980..3465)
                     /gene="msrB"
                     /locus_tag="PK754_13140"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNB10494.1"
                     /translation="MKKLLFAVWLMMTACSNAQHPSTHTVQNSTRKDQIVKSEEEWKK
                     ELSPEQYKVLRQQGTERAFTGKYWDHHEKGMYICAACGLELFDSQTKYDSGSGWPSFY
                     QPADKEVVSETHDTSYGMNRVEVHCARCGGHLGHVFEDGPDPTGLRYCINSLSLDFKK
                     K"
     gene            complement(3569..>4408)
                     /locus_tag="PK754_13145"
     CDS             complement(3569..>4408)
                     /locus_tag="PK754_13145"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNB10495.1"
                     /translation="PGPRILTAIQPIVPTGAYDLVGYSPYYEFPPIAFHADGADEIRT
                     QIRRLSKLGADVIKIYMESYEKRETSKDSLTGAMHYTPDELRVLVDEAHHNGLRVAAH
                     VYSDTAARLAIAVGVNSIEHGLYLSDETCRRMAKKNIYYVPTLMVYELWRDGIIFSPV
                     SDENKRKLARTVKQHTLSFQRALKAGVKIVFGTDTFELPGTNAQELERMTAYGMNPTD
                     VLRAATYASAMLLGVEKITGSIEDGKMADLIAVNGDPTENIAVMRNVVFVMKDGVIYK
                     NEP"
BASE COUNT         1223 a         1063 c          886 g         1236 t
ORIGIN      
        1 gaagttgatt accggcaaaa aggcctcttg tggtacagcg tgtataaagt acgtttttcc
       61 ggtttataca catttaccaa tccatccgct aaagaagaag atgtaacgat tgactttact
      121 tttcccgccg ctcaggcgat ctatgacgat ttcgttttcc aatgggatgg ccaagagctg
      181 ttaaaaacag aaaccattcg cgaatcttcc gatgatcctt atcgtcaaga cagcaaaccg
      241 ggttctttac gtgcgaatat caagatgaaa ccgggtcaga tcgctgtttt aaaagtaggt
      301 tatacttccc aaggaaccgg gacatgggaa tacagtgtcg gcgaaggcac caaacgggtt
      361 acggattttc aattgactgc acagacgcat ttcaaagaat ttgatattcc ggaattcagt
      421 atttctcccg gcactaagac tgaaacggca gacggttggt cattagtttg gcagtacaaa
      481 gacatcatct ccgacgttcg tatcggtctc ggtatgccta aaaaattaga ccccggaccg
      541 gtagccgaac gcatcagctt ttttgctccg gtatcgttac tatttttctt ctgcattctt
      601 attattatcg gagcgatacg tgatgtgcga atgcatccga tgaattattt tttcctcgcg
      661 gccgcctttt tttcgtttca tctgcttttc gcgtatctcg tggatcatct ggcacttgaa
      721 ctttcgtttg cgatttcatc cattgtctcc gtcacactcg tcgccgctta tatgcgcctt
      781 gtaacctcgt ggccttatac cgtacgtgtg gtgatgccta cgcaagtgat ttatctgatc
      841 ctattttcct atagtttctt tttcgaaggc tacaccggac tcacgatcac gatcggtgct
      901 atcgtaacgc tttttattct tatgcagatg acaggtaaaa cggattggaa tgctattttc
      961 aaaaaagata tcaaatcata aaaatcgtat ttagcaaaaa caaaaaacgc cgctcctatt
     1021 cgggcggcgt tttttattgc tcgaaaatct atgattagcg aagtgtcaaa ccaacgccaa
     1081 tgcttgtgga acgatatttg ccaaaactca tttccggatt cacattgatc aaccaaagca
     1141 gacggatatt tgcaccgaca ataccgcgca tactgttttt tccatccaca gaaaatttca
     1201 ccttagttcc ttcaggcacg ccgcttgctg ttccggtggt ttcgtagtta atatccatag
     1261 atgaggattc gataccgacg ccaccataca agccgagttt aatgattaaa atgtttaaac
     1321 tcttaccggc aagtatatca aaggatgtcg ctttagcttc gaacacatca ccaatagaca
     1381 ttttttgata aaaggcaccc gcagcaatat ccaaaggcaa gctgatcggc agccattgat
     1441 tgatgttatg tttagcgccc aaaccaaaca attttacttt gccaagttta ccggcaggaa
     1501 tacccggtac aaaacgaaga atcacatccg ttttgaaagg cacacctaca gtcagctgcg
     1561 gagccacaaa aaaaccgctt gcgccggccg gagcatctat accacccgga agaacaatat
     1621 tcgtttgatc atttggaata ccgccaagat aatatgtggt aggatcttta gatccgatag
     1681 ctgtatttgc ttcgttggat gcggccggta catacccgtt gggaacaacc ggggtgtatg
     1741 ttttttcaga ggaaggggca aaagccatag ccgtcactaa ttggaaatca aagccaagaa
     1801 cactgtgagt ttccggtgaa cgaaaaatac cgctattcat cgctacacca aaaccagaaa
     1861 caataggttt gatataagat tgagcgttgg cgctagccat gcttttgatc tttgcttcca
     1921 gatcatcgcc cgccatggca cgcccagtcg taacggttaa aatcaaacct gcaagcatca
     1981 gtcgcaaaaa tgacttcatg tctctctcct tcaagtaatt tagtgaaaaa tgaaaatctt
     2041 taggttaatg agaagcgtgt acaaatttaa ctgtaacttt gcgtaattgc aatacgcata
     2101 attactgtga aaataattaa ccaactagca tgccaaacta cagaaatcga aaaaacaaca
     2161 ggttaattat gataaaaaaa caaagcgtcg catatatgcg attattcaca gaaaacatgc
     2221 gtcgcaaaaa cgctactttt ttcgagctac gatgatagcc gaactgccaa aactcaggag
     2281 atgggccgtt ttgatttcat attcaaaacg tatgagtgac tcttgccatt tttcaaacca
     2341 attggaactt tgtatgagcg aggttaaccc gccatcaaaa acttcatgcc attcaggaaa
     2401 aaatcgcatg cctacaaaat tcatcgcgcg gttatatttc cgccaaatgg cgcgcgctac
     2461 cgagttgggt ggataataaa aatcatgcac ggctatcaca cctgatgact tgagatgcgg
     2521 atttatattg gaaagcaaaa tctcgggatc aacatattta gggatgtacg acgaaataac
     2581 ggcatcaaaa attccatcaa gtttcacatc ttccgcatta ccgagtaaaa acgaagcccg
     2641 aaggtttttc gatcgaatac gacgttcata tattttcaaa taatcatctg taatatccac
     2701 gccgacaatg tcggcatccg gacatcgttt ggcaattcct tccgtcacga tacctgttcc
     2761 acaggccaga tctaaaacgc ggcgactgtc cttgggtatg tgccgtaaca acatccgctt
     2821 ccagtgataa tctaaaccta aagtaaatcc gtgtacgacg cgatcatagg aatggccggt
     2881 tcccggaaaa aaagaacgga tgatggatga gcggtttttt atagtatccc ttccgacatc
     2941 cttcatccgt gtgttcctta gaccaaaatt atatttatct tatttcttct taaaatccaa
     3001 cgaaagtgaa ttgatacaat aacgcaatcc tgtcggatcc ggtccgtctt caaacacatg
     3061 accgagatgc ccaccgcagc gtgcacagtg cacttctaca cgattcatcc cgtaggaggt
     3121 atcgtgcgtt tccgatacga cttctttgtc cgccggttga taaaaactgg gccacccgct
     3181 tccggaatcg tatttggttt gcgagtcaaa aagctccagt ccgcaagccg cacatatata
     3241 catacccttt tcatggtgat cccagtattt tcccgtaaat gcgcgctcgg ttccctgctg
     3301 acgcaggact ttatactgtt ccggcgataa ttcttttttc cactcctctt cgcttttcac
     3361 gatctgatcc tttcgggttg agttttgtac cgtatgggtt gacggatgct gtgcattgga
     3421 acacgccgtc atcatgagcc aaactgcaaa aagtaacttc ttcattcttt tttctttcga
     3481 tggtttctcg acttaatcta acaccgagac gattgcaata ttcccatcat attgatattg
     3541 tgatccgtat cacacgcatt ggtatccatc agggctcgtt tttatatata accccatctt
     3601 tcattacaaa tactacatta cgcatcacag ctatattttc cgtcggatca ccatttacgg
     3661 caataagatc ggccattttt ccatcttcta ttgatcctgt gattttttct acacccaaga
     3721 gcattgccga cgcatacgtc gctgcacgta atacatccgt cggattcatt ccgtacgcgg
     3781 tcatccgctc aagttcctgt gcattggtac ccggtaattc aaacgtatcc gtcccgaaaa
     3841 caattttgac cccagccttc aaagcacgct gaaacgaaag cgtatgttgt tttacggtgc
     3901 gcgccagttt gcgtttgttt tcgtccgaca ccgggctaaa gataataccg tcgcgccaca
     3961 attcgtatac catcaaagtc ggtacataat aaatattctt tttcgccatc cgccggcatg
     4021 tctcgtcact aagatagagg ccgtgctcga tcgaattgac accgacagca atcgccagtc
     4081 gcgctgccgt atccgaatac acgtgggccg caacacgtaa accgttatgg tgcgcttcat
     4141 ccacaagcac tcgtaattcg tccggcgtgt aatgcattgc tcccgtcagc gaatccttac
     4201 ttgtttctct tttttcatac gattccatat atattttgat cacatccgca ccaagtttgg
     4261 acagacgtcg aatttgcgta cgaatttcat ccgcgccgtc ggcgtgaaat gcaatcggcg
     4321 gaaactcata atacggcgaa taaccgacaa gatcgtacgc ccccgtcgga acaataggtt
     4381 gaatggccgt taatatccgg ggaccggg
//
LOCUS       DAOQNQ010000270         4407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_8719_length_4407_cov_6.831572, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000270 DAOQNQ010000000
VERSION     DAOQNQ010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8719_length_4407_cov_6.831572"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PK754_13150"
     CDS             <1..247
                     /locus_tag="PK754_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNB10496.1"
                     /translation="PAVYATYNAGKTWKRLDKGFPKKDAYWTVFRQAMTCDQKSKVGL
                     YLGTTSGEVWGSTDEGRSWSCLVRHLPEIFSLEVLTR"
     gene            254..511
                     /locus_tag="PK754_13155"
     CDS             254..511
                     /locus_tag="PK754_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNB10497.1"
                     /translation="MVIHIPNPLRSYTSDEKVQAEGHTIVEVLKNLDAQFPGIRFRVI
                     DEQDRIRRHIKIFLNEEMVDNLKEPVESKDTLHIICALSGG"
     gene            569..2725
                     /locus_tag="PK754_13160"
     CDS             569..2725
                     /locus_tag="PK754_13160"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNB10498.1"
                     /translation="MRQLFLWQCITLCAFVLHIACSDLKAQSYLPVIELTEQSTVMDV
                     TTKMEIFEDAGGQNQIEDVIRDNVVFKINDQAMPNFGFTKSAYWVRFVVRSSSEAHWF
                     LHLDFPLMDELVIYRLSNGLPEPIRITGRMREFSSRDIPHRTFAVHLPLHAGSTDTLY
                     ARFRTDDTMTLPLYLIRADIFRERDHTQQFGFGLYYGIMLVMALYNVFIFLKLRDRSY
                     LYYVLYIASFGMLLMYQNGISYELFPWAQWRMDPFWSSMASLWGIQFSRHYLLSHRFT
                     PRLDRVLKILLMMSVVSVVISVVAPYRFSIWPGVVIGILIFPLMISTAIIAIRKNYTP
                     GKIFLTAWIVLLTGIVLNAFMSMGLLPANDFTLYGIQVGSALETVLLSLGLANRISVL
                     KHEKEEAEKKLVETELRMKAAVVQQLEEKVRERTALLEAQKQELAQTLEVLKNTEVQL
                     VQTAKMSALGQLIAGVAHEINNPITFIYSNLYPLEDYIEQFKQLAKAAIEHGLHTQLQ
                     DEGVNKKLNNLDELFDDSDRILKSFRLGTQRIRNIVQDLQKFEHADESEKIPVDMNDE
                     IRSVLKLITPQYRDQIEINTQWGPIPEFYCTPSHFHQIFMDIFINAAQAIIQRNKDDK
                     RPKGNLHVATYVDESQAWIGIDIRDDGTGIAQEHITKIFDPFFTTKPVGEGTGLGLAI
                     AYRLIEKNRGTIQVQSKVRDFTQFTIRLPINSLIKE"
     gene            2727..3641
                     /locus_tag="PK754_13165"
     CDS             2727..3641
                     /locus_tag="PK754_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10499.1"
                     /translation="MKRYRWLVITGSVILSLAACEVMVRYTFLPLSSLHNGAVNVPDA
                     RGYALRPGASVEFRGIHEPLPQPVQWIVNKQGLRADRTDSVPRISAKKRIAVFGDSEV
                     FGWSVNYENTFESVMMHEDPSLEVLNFGVPGYNAENIADHVERMAPGFSLDGMIYLIN
                     PNDLDPPLAYREATIDSELLRRIAFAYMSLTETDYVEHRKSDSVRIGFLKQVDRIVQY
                     TQTHHIPLMLVCMNEDELRLLSDKRPLTREAAVTAISVYDVFTQSPKWDVHLSASGHN
                     TLGQRMLSEWRNFASGLPMKDSKTLANN"
     gene            3762..>4407
                     /locus_tag="PK754_13170"
     CDS             3762..>4407
                     /locus_tag="PK754_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10500.1"
                     /translation="MRTHRFVLKTILMALCASSYVFAQSKVKLQYNDLQNILTELRSL
                     ERIETPEGVPDHSASSVAHSLERLSALRLRYSKIDTSGWPVEQRIDHALFGAEINGLD
                     FHLRVLRPWSRDPAYYALIWMEESDTPAHEGPLCHASIDVWRYTFPLSSTDEKKLTRQ
                     LQVIPPLLRQAQSNLTENARDLWIAGTAQVREQISFLDEIKSQSQTAGAELHTAI"
BASE COUNT         1196 a          904 c         1052 g         1255 t
ORIGIN      
        1 accggcagtc tatgcaacgt acaatgcggg aaaaacgtgg aaacgtttag ataaggggtt
       61 tccgaaaaaa gatgcttact ggacggtgtt tcgtcaagcg atgacatgcg accaaaaaag
      121 taaagtcggt ttatatctcg gaacgacctc cggagaggtg tggggcagta ccgatgaagg
      181 gcgatcctgg tcgtgtttag ttcggcactt gccggagatt ttttcacttg aagttttaac
      241 ccggtgatga tgtatggtaa tacacattcc caatcctttg cgttcatata catcggacga
      301 aaaagttcag gctgaaggtc atacgatagt ggaagttcta aaaaatttgg atgcacaatt
      361 tccgggaata cgatttcgtg tgatagatga acaagaccga atacgaaggc acatcaaaat
      421 ttttcttaat gaagagatgg tcgataacct taaagaaccg gttgaatcca aagatacatt
      481 gcacatcatt tgcgcgttaa gcggaggttg acagttctac tgttagcccg ggcattataa
      541 aacgcatcat ttaattttaa tcttactcat gcgacaatta tttttatggc agtgcattac
      601 gctatgtgca tttgttttgc atatcgcatg ttctgatcta aaagcacaaa gctatctgcc
      661 ggtcattgag ttgacagaac aatccacggt aatggatgtg accacaaaaa tggagatttt
      721 tgaagatgcg ggcggacaaa atcaaattga ggatgttatc cgtgataatg ttgtgtttaa
      781 aataaacgat caggctatgc ctaattttgg atttacaaaa tccgcttatt gggttcgttt
      841 tgtcgtgcgg tcatcatcgg aggcacattg gtttttgcat ctggattttc ctctgatgga
      901 tgaattggtg atatacagat tatcgaacgg gttgccggaa ccaatccgaa taaccggaag
      961 aatgcgtgaa ttttcctcgc gcgatattcc gcatcgtacg ttcgcagttc atttgccttt
     1021 gcatgcgggt tcgacggata ctttgtacgc acggtttcgt acggatgata cgatgacgtt
     1081 gcccttgtat cttattcggg cggatatttt tcgcgaaaga gaccatacgc agcagtttgg
     1141 tttcggtttg tactacggta ttatgctcgt gatggcattg tataatgttt ttatcttttt
     1201 aaaactccgc gaccgatctt acctctatta tgttttatat attgccagtt tcgggatgct
     1261 cctgatgtat caaaacggca ttagctatga actttttccg tgggctcaat ggcgaatgga
     1321 tccgttttgg agttcgatgg ctagtttgtg gggcattcag ttttcgaggc attacctgct
     1381 tagtcatcgt tttacgccgc gcctggatcg cgtgcttaag attttgttaa tgatgtctgt
     1441 cgtatccgtt gtgatttctg ttgtcgcccc ttatcggttt tcgatttggc ccggtgtagt
     1501 gatcgggata ctgattttcc ctttaatgat ttctacggca attatagcga ttcgtaaaaa
     1561 ttatacgccc ggaaagatat ttctcacggc atggattgtt ttgcttacag gtattgttct
     1621 taacgccttc atgagcatgg gattgcttcc ggccaacgat ttcactcttt atggcattca
     1681 ggtgggttca gcgttagaaa cggttttact ttcactcggc cttgccaacc gtatcagtgt
     1741 actcaaacat gaaaaagaag aagctgaaaa aaaattagtc gaaacagaac ttcgtatgaa
     1801 agccgctgtc gttcaacaac ttgaagaaaa agtgcgtgaa cgaacggccc ttttggaagc
     1861 gcaaaaacaa gaactggcgc agacgctaga agttctcaaa aacacagaag tccaattggt
     1921 acaaaccgca aagatgtcgg ctttgggtca gcttattgcc ggagtggcac atgagatcaa
     1981 taatccgata acttttattt actccaatct gtaccctctg gaagattata tcgaacagtt
     2041 caaacaactt gccaaagcgg cgattgagca cggactgcac acgcaattgc aagatgaagg
     2101 tgtaaacaaa aaactaaaca atctggatga attgtttgat gattcggatc gcatactcaa
     2161 aagttttcgt ttaggtacgc agcgcatacg aaacatcgtg caggatttac aaaaatttga
     2221 acatgcggat gagagtgaaa aaataccggt tgatatgaac gatgagatcc gcagcgttct
     2281 gaagctcata acaccgcaat accgcgatca gatagaaatc aacacgcaat ggggtcctat
     2341 tcctgagttc tattgcacgc cttcgcattt tcatcagatc tttatggata tttttattaa
     2401 tgcggcccag gccattatcc agcggaataa agacgataaa agacctaagg gcaatcttca
     2461 cgttgctacg tatgtagatg aaagccaggc atggatcgga atagatatcc gtgatgacgg
     2521 aacaggtatc gcgcaggagc atattacgaa aattttcgat ccttttttta cgaccaaacc
     2581 ggtcggggag ggaacagggc tgggtttggc catagcgtac cgattgatcg aaaaaaaccg
     2641 cggcacgatt caagtgcaat ccaaagtaag ggactttact caatttacta ttcgattacc
     2701 tatcaactca ttaataaagg agtgacgtga aacgctatcg ttggctggtg ataacagggt
     2761 cggttatttt aagtttggcg gcgtgtgaag taatggtgcg gtacactttt ttacctcttt
     2821 caagtctgca caatggtgct gtcaatgttc cggatgcaag gggttatgcg ttgcgtcccg
     2881 gggcttccgt cgaatttcgc ggtatacacg aaccgttgcc acaaccggtg caatggatcg
     2941 ttaataaaca aggtttgcgg gctgatcgta cggactcagt gccgcggatc tccgccaaaa
     3001 aacgtattgc cgtatttggt gacagtgaag tattcggttg gtcggtaaat tacgaaaata
     3061 catttgaatc ggtaatgatg catgaggatc cgtcgctgga ggtgctcaat ttcggtgtgc
     3121 ccggatacaa cgctgaaaat atagcggatc atgtggagcg aatggcgccg ggtttttcgt
     3181 tagacggtat gatttatttg atcaatccca atgatcttga tccgccattg gcttaccgtg
     3241 aagcaacgat tgattcggaa ttattgcgtc ggattgcatt tgcctatatg agtctgacgg
     3301 aaacggacta tgtagaacac cgaaaatccg acagcgttcg catcggattt ctcaaacagg
     3361 tagatcgcat tgtgcaatat actcaaacgc atcacattcc tttgatgtta gtttgtatga
     3421 atgaagatga gcttcgttta ttatcggata aacgaccttt gacccgggaa gccgcggtga
     3481 cggcgatttc cgtgtacgat gtatttaccc aatcgccgaa atgggatgtt catctttcag
     3541 cgagcggtca taatacattg ggtcaacgca tgcttagcga atggcgaaat tttgcatccg
     3601 gcttaccgat gaaagattca aagacattag cgaacaacta aaaacagaat cgaatggttt
     3661 ttagtgccat gtgtggaaca tgttttatta tttctttgtt ctcaagtata cgataagttt
     3721 tttgtatata taacatttca actatcaggg atgtgaaatt tatgagaaca catcggtttg
     3781 ttttaaagac tatactcatg gcgttgtgcg cgtcatccta cgtttttgcc caatctaaag
     3841 tcaagttaca atacaacgat cttcaaaata ttcttacgga acttcgatcg ctggagcgta
     3901 tcgaaacacc ggagggtgtg ccggaccatt ccgcttcgtc ggtagcgcat tctttggagc
     3961 gattgagtgc gttgcgtcta cggtattcta aaatagatac gtccgggtgg ccggtcgaac
     4021 agcgtattga tcatgccttg ttcggtgccg aaatcaacgg cctggatttt catctgcggg
     4081 ttcttcgtcc ctggtcacgc gacccggcgt attatgcatt gatctggatg gaagaaagtg
     4141 atacaccggc tcacgaaggc ccgctgtgtc atgcgtcgat tgatgtgtgg cgttacactt
     4201 ttccactgtc gtcaacggat gaaaaaaaac tcacccgcca actgcaagtc atcccaccgc
     4261 tgctacggca ggcccaatct aacctgaccg aaaatgcgcg cgatctctgg atcgccggta
     4321 cggcacaagt acgcgaacaa atcagctttc tggatgaaat caaatcgcaa tcgcaaaccg
     4381 ccggtgcgga actccatacc gccatca
//
LOCUS       DAOQNQ010000271         4390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_8771_length_4390_cov_6.230219, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000271 DAOQNQ010000000
VERSION     DAOQNQ010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8771_length_4390_cov_6.230219"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PK754_13175"
     CDS             <1..668
                     /locus_tag="PK754_13175"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HNB10501.1"
                     /translation="GHYYDKNLRIVGNISHLAAMIPQAVGTALASVKKKENAVVINYV
                     GDGGMNVGEFHEGLNMAAVWKLPFILIIENNQYAYSTPNTLQFACESPVDRALGYGIP
                     GVKVDGTDILEVYRVCKEAFERARRGEGPTLIESVTMRMRGHAEHDGHEYYPPGILEK
                     WEKKDPIMQFEKFLKSEKLITDKMIEDIDTSTKQEIDEAIEYADKAPWPEPSQAATWV
                     YAE"
     gene            708..1736
                     /locus_tag="PK754_13180"
     CDS             708..1736
                     /locus_tag="PK754_13180"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HNB10502.1"
                     /translation="MTGQSKTSTKPYAHSTELDLKKYEPTKEQMKEMYYFIMLNRAFD
                     DRIAKLYRQGKIIGAAYGSRGQEATSVGSTYALGPDDIVGPIIRNAGSIIVRGLPVKN
                     FLSNFVGRSTTPTRGRDGNSHLGDLNMGVFAPISHLGSLISNIAGCALAFKMKNEPRV
                     ALTYIGDGGTSTAEFHEGMNMAAVMKLPFICIIENNNWAYSTPTQTQAMIKDFAIKGK
                     AYGMEAIVVDGNDVFEVFRTTRYAAERCRKGKGPILIEAKTMRMKGHAEHDDAFYVPK
                     EQFAEWQKKDPIARAEKFLLDKKYMTATENEALKQRVAKDMEEAEEFALNAPFPAMDE
                     AATGVYAD"
     gene            1749..2810
                     /locus_tag="PK754_13185"
     CDS             1749..2810
                     /locus_tag="PK754_13185"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNB10503.1"
                     /translation="MKKLFLIFVLFVHSAFAQTSFAIIPFTGTADVGVNDREAFVSGL
                     YQGMTNSGRYRIVDRNLIEQVLKEQNFQLSDLTEQKKVIEFGKIAGAEKIITGKMFRY
                     SSTQPAITFSVIDVSTSQVEFSKEITYNNYTFSNLSSFLVSQLIDKYPLVGRVLGKSG
                     DVYIINIGLNQNIKNGTRIFVARNNVLKGDDGEILMEEYKRVGILEVTLADKARSQCK
                     LRSLVESNVIIQKDDMISPDPIPERPALVLDKPFWSGYSKGELLLDDDMSKKQYLSVT
                     SALGETYKDGRLFINTTHLNSGHGYCYYPAPFDAVGDFVCEAEITFEKNTEKYNRVTF
                     IFRNVGAYGEGNGYSLPVV"
     gene            complement(2812..3672)
                     /locus_tag="PK754_13190"
     CDS             complement(2812..3672)
                     /locus_tag="PK754_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HNB10504.1"
                     /translation="MVHEHLSDMLTAQGNIPKPGPRPKLSDAAIIALSLTSEILSIDS
                     ENRLFMQLRQYPKVFPTLIDRSGYNVRRRKLFSYTNHLRNTILQKISSSESVFIIDTM
                     PLPVCKLARANRSRICKESFETAPDKGFCAAQQTYFHGYKLHSVCTQKGVFTSFDLSK
                     ASVHDIHYLKNVKEHMANAILLGDKGYLSEDIQIDLFTSVRINLQTPLRSNQKNYVKQ
                     PSILRKTRKRIETLFSQLCDQFMIHRNYAKSFDGFKARILAKITALTISQFFNYQNQK
                     NLNLIKYAFI"
     gene            3859..4098
                     /locus_tag="PK754_13195"
     CDS             3859..4098
                     /locus_tag="PK754_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10505.1"
                     /translation="MRNGFLFPIIKFTSTPTLKRGTSKNTLRVIAKGSKFDLYFNDQF
                     LAAFEHEAIEKGGFGIWSGQGSHISVDNVKIWNIK"
     gene            complement(4119..>4390)
                     /locus_tag="PK754_13200"
     CDS             complement(4119..>4390)
                     /locus_tag="PK754_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNB10506.1"
                     /translation="KAVIDDDGHTLFRGERMAVCDKTFGIYTREKGPYANDIFAVNPK
                     EIISLESAKLFNCNKNARRHARETKGMDYNETILSEACCGPDGCC"
BASE COUNT         1303 a          828 c         1060 g         1199 t
ORIGIN      
        1 cgggacatta ttatgataaa aatctccgaa tcgtcggtaa tatcagccat ttggcggcaa
       61 tgatacctca agccgtcggt acggcattgg cgtccgtaaa gaaaaaagaa aatgcggtcg
      121 tgatcaatta tgtcggtgac ggcggtatga acgtcggtga atttcacgaa ggcctcaata
      181 tggccgcggt atggaaattg ccctttatcc tgatcatcga aaataatcag tacgcgtatt
      241 ccacgcccaa tacattacag tttgcctgcg aaagtcccgt ggatcgtgca ttagggtatg
      301 gcatacccgg cgtcaaagta gatggtacgg acattttgga agtgtaccgt gtttgtaagg
      361 aagcatttga acgggcgcgt cgcggtgaag ggccgacgtt gatcgaatcg gttacgatgc
      421 gtatgcgcgg gcacgccgaa cacgacggcc atgaatatta tcctccgggg attttggaaa
      481 aatgggagaa gaaagatccg atcatgcagt ttgagaaatt tcttaaatcg gagaaattaa
      541 tcaccgataa aatgatcgaa gacattgata cgtcaaccaa acaagaaatt gacgaagcga
      601 tcgaatatgc cgacaaagcg ccgtggccag agccttcgca agcggcgacg tgggtgtacg
      661 cggaataatt aaatcctaat attaacattg gtaaaaaata cttacgcatg accggtcaaa
      721 gtaaaacatc gacaaaacca tacgctcatt cgacggaact ggatctgaaa aaatacgaac
      781 cgaccaaaga gcagatgaaa gaaatgtact attttatcat gctcaatcgc gcgttcgatg
      841 atcgtatcgc caaactttac cgccaaggta aaatcatcgg agccgcatac ggcagccgcg
      901 ggcaggaagc caccagtgtg ggttcgacat atgcgttggg tccggatgat atcgtcgggc
      961 cgattattcg taatgcgggt tccatcatcg ttcgcgggct accggtcaaa aatttcttgt
     1021 caaattttgt cggtcgttcg accactccga cgcgtggccg cgacggcaat tcacacttgg
     1081 gtgatttgaa tatgggcgtc ttcgctccga tttcacattt ggggagcctg atcagcaata
     1141 tcgcaggttg tgcactggca ttcaaaatga aaaacgaacc gcgtgtggcg ctgacttaca
     1201 tcggggacgg aggtacgtcc acagccgagt tccatgaagg tatgaatatg gccgccgtga
     1261 tgaaattacc ttttatttgt atcatcgaaa ataataactg ggcatattcg acgccgacgc
     1321 aaacccaagc gatgatcaaa gattttgcca tcaaaggcaa agcttacggc atggaggcca
     1381 tcgtcgtgga cggcaatgat gtttttgagg tgtttcgtac gacacggtac gcggcagagc
     1441 gctgtcgaaa aggcaaggga cctattttga tcgaagcgaa aaccatgcgc atgaaaggtc
     1501 acgcggagca cgatgatgcg ttttatgttc ccaaagaaca atttgcggaa tggcagaaaa
     1561 aagatccgat tgcgcgtgcg gaaaaatttc ttctcgataa aaaatacatg accgcaacgg
     1621 aaaatgaggc cttaaaacaa cgtgtggcga aagatatgga agaagcggaa gaatttgcac
     1681 ttaatgcgcc gtttccagcc atggacgaag cggcgaccgg tgtatacgct gattaaggag
     1741 gagaaacgat gaaaaaatta tttctaatat ttgttttgtt cgttcattcg gcatttgctc
     1801 aaacctcatt tgccattatt cctttcacgg gtactgctga tgtaggagtt aatgatcgcg
     1861 aagcttttgt tagcggactc tatcaaggga tgacgaatag cggtcgttac cgtatcgtag
     1921 atcgcaacct gatcgaacaa gtattgaaag aacaaaattt tcagttgagt gacttaacgg
     1981 agcagaaaaa agttatcgaa ttcggaaaga ttgccggcgc cgaaaaaatt ataactggta
     2041 aaatgttcag atattcttcc acacaaccgg cgattacatt ttccgtaatt gatgtttcaa
     2101 ccagtcaggt tgaattttct aaagaaatca catataacaa ttatacgttt tcaaacctaa
     2161 gcagtttttt ggtatctcag ttgattgaca agtatccgct ggtgggtaga gtgcttggta
     2221 aatccggaga tgtgtatatc atcaatattg gcctcaatca gaatataaaa aacggaacgc
     2281 gcatttttgt ggctagaaat aatgtactca aaggcgacga cggcgaaatt ttgatggaag
     2341 agtataagcg cgtcggtatt ttagaggtga cattagctga caaagcgcgt tcgcaatgca
     2401 agttacgtag tttggttgaa agcaatgtta tcattcaaaa agacgatatg atttctccgg
     2461 atccgatacc ggagcgtccg gcacttgttt tggataaacc tttttggtct ggttattcaa
     2521 aaggagagct gcttttagat gatgatatga gtaaaaagca gtatctatct gtaactagcg
     2581 ctttgggcga aacctacaaa gatggacggc tttttattaa tacgacacat ctgaattccg
     2641 gtcatggtta ctgttattat ccggcgcctt ttgatgccgt gggtgatttt gtatgcgaag
     2701 cagaaatcac gtttgaaaaa aacactgaaa agtataatag ggtgactttt atctttagaa
     2761 atgtcggtgc gtatggcgaa ggaaatggtt attcgttacc cgttgtgtga attaaatgaa
     2821 agcatatttg ataaggttta gatttttttg attttggtaa ttgaagaatt gagaaatcgt
     2881 aagcgcagta attttagcaa ggatacgagc tttaaagccg tcaaaggact tggcataatt
     2941 tcggtgaatc ataaattgat cacaaagctg agagaaaaga gtttcaatgc gtttacgtgt
     3001 tttgcgaagg atacttggtt gtttaacata gtttttctga ttggaacgca gaggagtttg
     3061 caggttgata cggacagagg taaataggtc aatttgaata tcttctgaga gataaccttt
     3121 gtcgccaaga agaatggcat tagccatatg ttctttcaca tttttgaggt agtgaatatc
     3181 atgcacggag gctttggaga gatcaaagga agtaaagacg cctttttggg tacatacgct
     3241 atggagtttg tagccgtgaa aataggtttg ctgggcggca caaaaacctt tatccggagc
     3301 ggtttcaaag gactctttac agattcggga gcgattagcg cgcgctaatt tgcagacggg
     3361 caaaggcatg gtatcgatga taaataccga ttcggaggaa gaaatctttt ggagaatcgt
     3421 attccgtaga tggttggtat aggagaatag tttacgtcga cgtacattgt atccggaacg
     3481 gtcgatgagc gtgggaaaga ctttcggata ttgtcgcagt tgcataaaca agcgattttc
     3541 actatcaatg gagaggattt cgctcgtcaa actcagggca ataatggcgg catcggataa
     3601 tttgggacga ggaccgggtt tagggatatt tccttgagcc gtaagcatgt cggataaatg
     3661 ttcgtgaacc atttttaaaa cacgatcaaa ggtttgtttg aggttgtgca tgaactttgg
     3721 tctcctaaaa aagaatttga aattactctt tttagaaaag ccaaattcat gccaacttct
     3781 tttattaaat cataattaat tcacacaacg ggttattcgt tatactttac caatgatggt
     3841 tcctaccaaa tggattatat gcgtaacggg tttttgttcc cgattatcaa atttacttca
     3901 accccaacat tgaaacgcgg cacgtcaaaa aatacattac gcgtcatagc caaaggttcg
     3961 aaatttgatc tatattttaa tgatcagttt ttagcagcat ttgagcatga ggccatcgaa
     4021 aaaggcggat tcggaatttg gtccggacag ggtagccata tatcggtgga caatgtcaaa
     4081 atctggaata ttaaataaat tttttatttc aagtcctatc aacagcagcc atccggcccg
     4141 caacaggctt cgctcagaat agtttcattg taatccatac ctttagtttc gcgtgcgtga
     4201 cggcgagcat tcttattgca attaaagagt tttgccgatt ctaaagaaat aatctcttta
     4261 gggtttaccg caaaaatatc attggcgtac ggcccttttt cccgtgtata aatgccgaaa
     4321 gttttatcac acacggccat acgctccccg cgaaaaagtg tgtgtccgtc atcatctata
     4381 acggctttcc
//
LOCUS       DAOQNQ010000272         4374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_8828_length_4374_cov_5.185691, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000272 DAOQNQ010000000
VERSION     DAOQNQ010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8828_length_4374_cov_5.185691"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2048)
                     /locus_tag="PK754_13205"
     CDS             complement(<1..2048)
                     /locus_tag="PK754_13205"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB10507.1"
                     /translation="MKKHIAQLIVWICWSGTIVLAQSDRIKFERLSIGEGLSQSKVFS
                     IYQDHKGFLWIGTMDGLNRYDGYGFTVFRHDPRDSTTISENYVWSIIEDKEENLWIGT
                     NGGGLNRFDRKTQKFKPYRNIADDPHSISDNRVWALYVDRDGVLWVGTRYAGLCKYDR
                     ATDRFIAYKNNPQDSTSLSHNYVMTVFEDSKNNFWIGTNGGGLNRMDRATGKFTRYRY
                     KPGQRSPVPEERIWALSEDREGYLWVGTNNNGLSRWNVATGEFEIFRHSSADPNSIAD
                     NWIKSIYFDRQDRLWICTNGGVSIYDKTTQKFYNYRNNPADPSSISNNFVNAFCEDRM
                     GILWFGTYGGGVNMYDPYKERFKHYHLDLSQPQGLSGNIVISFAEDHEGIIWIGTDDA
                     GLNRYDPKKQTYRHYKHNPADAKSISNNRVSVIYEDRKHNLWVGTRGGGLSRFDRKTE
                     QFKNYRIDPISSAQSNNVVFAILEDTSGTLWVGTRAIGLQIFDEKKGEFRRVRYDGRN
                     PADNGVQVLMQDSRGYIWVGTSGGGIVRLDPKTYAFTRFVHDPSNPNSLSHNAIVSIL
                     EDAQGKIWVGTSGGLNRIDPSNDKILQITEKHGLPNDVIASIQQDGNGHLWISTNKGL
                     SRIDINNFENGGPTAHNRIRNFDVFDGLQSNEFSMNAGLKARDGWMYFGGINGFNVFH
                     P"
     gene            complement(2048..>4374)
                     /locus_tag="PK754_13210"
     CDS             complement(2048..>4374)
                     /locus_tag="PK754_13210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB10508.1"
                     /translation="EDSDGILWIGTHDGLNRYDAGTDRFTAYRAGPGRLSSNEVRTIA
                     EDKNGMIWLGTEGGGLNKFDRRTNRFTVYQHRPGDSLSIAGNDISVLLSDKYNQLWIG
                     TFGDGLDKLIVENGKEIFVHYRNVLHRSQSLSHNEVSSILMDSEEKIWVGTFGGGLNR
                     LDSVQDRTGYASFKRIAGRGESNSSLGSDVIFAISEDSDKNIWIGASSGLNRIVRTTD
                     GSLKFYNYYEKDGLPDNYVYAILPDEHGNLWISTVQGLSRARLIQDENDQSEKLMFRN
                     FDESDGLQNNEFKYGSYYKSRSGEIFFGGTNGFNRFYPKLAMDNPHIPQIVLTAFKTM
                     HKSDKAFVDAYIESVYRHDPIILDYNEDFSFEFASLDFTEPAKNQYAYKLEGFDKNWI
                     LAGTRRYVNYTNLDAGNYVFNVRGSNNDGVWNEQGISIPIRIVPPFWKTWWFIALSFS
                     ALMGLLWAFIRYRVRVIELQKNKLEVEVSNRTREIKAQRDDLKAINSQLEKTLQELNE
                     TQSQLMHAEKMASLGQMVAGIAHEINNPLTVVDGNLHLLEENVLRWDHIITEYERLIE
                     TADPEKIKMGMMEIRERHEYEYVRQETMKILDSCKNGSQRIKHIVQDLRKFSRTDAMG
                     FIPADLHEGIESTLTVLKSMLSDRITVHKQFGTIPMLDCYPGLLNQVFLNILTNAIEA
                     IPNEGIITIKTYLSENNIRIHIKDNGIGMHPSMVKSIFDPFFTTKEIGKGMGLGLSIS
                     YGIIKKHNGDIEVISEEGKGSEFIISLPVIKRVS"
BASE COUNT         1182 a         1072 c          853 g         1267 t
ORIGIN      
        1 ggatgaaaaa cgttgaagcc gttgatccct ccgaaataca tccatccatc gcgcgccttg
       61 agtccagcat tcatgctaaa ttcattactt tgcaagccat caaacacatc aaaattacga
      121 attcgattgt gcgcggtagg gccaccgttt tcaaaattat tgatgtcaat acgagacaac
      181 cctttgttcg tactaatcca caaatgtcca ttaccgtctt gttggattga agcaatgaca
      241 tcattgggta ggccatgctt ctctgtgatt tgtaaaatct tatcattgga tggatcaatg
      301 cgatttaatc cgccgctggt accgacccaa atctttcctt gcgcgtcttc gagtattgat
      361 acgatggcat tatgactcag actgttcgga ttggacggat catgcacaaa gcgcgtaaac
      421 gcatacgttt tcggatccag ccgtacaata ccgccaccgc tcgttcccac ccatatatat
      481 ccccggctat cctgcatcaa tacctggacg ccattgtccg ccggatttcg cccgtcgtaa
      541 cgtacgcgcc gaaattcacc ttttttttcg tcaaagattt gtaacccgat cgcgcgtgta
      601 ccgacccata atgttcctga ggtatcttcc aatatggcaa aaaccacatt attgctttgt
      661 gccgatgata taggatcaat tcgataattt ttaaattgtt cggtcttgcg atcaaaacga
      721 ctcagtcccc cgccacgcgt acctacccat aggttatgtt tacggtcttc ataaatcacc
      781 gatacgcgat tattactaat actcttcgca tcggcaggat tatgtttata gtggcggtag
      841 gtctgttttt tcggatcata acgattaagc cctgcgtcgt cagtcccgat ccaaatgatg
      901 ccctcatgat cttcggcaaa actaatcaca atatttccac tgagcccttg cggttgcgaa
      961 agatccaagt gataatgctt aaatcgttct ttgtatgggt cgtacatatt gacaccgcca
     1021 ccatacgtgc caaaccataa aatacccatg cgatcttcgc aaaaggcgtt tacaaaatta
     1081 ttactgatgc tggacggatc cgcagggtta ttacgataat tatagaactt ttgagtcgtc
     1141 ttatcataaa tgcttacacc cccattggtg caaatccaca aacgatcctg acggtcaaaa
     1201 taaattgatt tgatccaatt atccgcgatc gaattaggat ccgcagagct atgacggaat
     1261 atctcaaatt cgcccgttgc tacattccaa cgcgataacc cgttattatt tgttcctacc
     1321 cacaaataac cttcgcggtc ttccgataaa gcccatatgc gctcttcggg aacgggcgaa
     1381 cgttgcccgg gcttataccg ataacgggta aattttcccg tagcgcgatc cattctattc
     1441 aagccgccgc cattggtacc aatccaaaaa ttattttttg aatcttcgaa aaccgtcatg
     1501 acataattat gactaaggct tgtcgagtcc tgcgggttat ttttatatgc aataaaacga
     1561 tccgttgcac gatcatactt acaaaggccg gcgtaacgcg tcccaaccca aagcacgccg
     1621 tcacgatcca catacaatgc ccacacgcgg ttatcactga tcgagtgcgg atcgtcggct
     1681 atgttacgat aaggtttgaa cttttgcgtc ttacgatcaa atcggtttaa tcctccgccg
     1741 ttagtaccga tccacaggtt ttcttctttg tcctcaataa tcgaccaaac gtagttttct
     1801 gatatcgtcg tcgaatcgcg cggatcatgg cgaaaaacgg taaaaccgta accatcatag
     1861 cgattgagcc cgtccatcgt accgatccac aagaatcctt tgtggtcttg ataaatcgaa
     1921 aaaactttac tttgcgacag gccttctccg atagataagc gttcgaattt aatacgatcc
     1981 gattgggcga gcacaatcgt gccactccaa caaatccaaa ctataagttg agcgatatgt
     2041 tttttcatca ggatacgcgt tttataaccg ggaggctgat aataaattca gagcctttac
     2101 cttcttcact tatcacttcg atgtctccat tatgtttctt tataatacca taactgatgg
     2161 ataaacccag ccccattcct ttaccaattt ctttcgttgt aaaaaatgga tcaaatatac
     2221 ttttaaccat tgatggatgc ataccgatac cgttatcttt gatatgaata cgaatattat
     2281 tttcagatag gtatgtttta atagtgataa taccctcgtt cggaatagct tctatggcat
     2341 tggttaaaat attaagaaat acctgattga gcaaaccggg ataacaatca agcatgggaa
     2401 ttgtgccgaa ctgcttatgg acggttatgc ggtcgctcaa catggatttc aaaaccgtca
     2461 gagtggactc aatcccctcg tgcaagtcgg ccgggataaa acccatcgca tcggtacgcg
     2521 aaaacttacg caaatcctga acgatatgtt tgatacgttg gctaccgttt ttacaagagt
     2581 ctagaatctt catcgtttcc tgccggacat attcgtactc atgccgttcg cgaatttcca
     2641 tcatacccat tttaattttc tcagggtcgg ccgtctcaat aagtcgctcg tactctgtga
     2701 taatgtgatc ccagcgcagt acgttttctt caagcaggtg gagattgcca tcgacaaccg
     2761 tcagcggatt attgatctca tgggcgatac cggctaccat ttgaccgagg gatgccattt
     2821 tttcggcatg catcagttgg gattgtgttt cgttgagttc ctgcaacgtt ttttcgagtt
     2881 gggaattaat ggctttcaaa tcgtcgcgtt gtgctttgat ttcgcgcgtc cggttggaca
     2941 cttcgacttc aagtttattt ttttggagtt cgatcacacg cacacggtag cgtatgaaag
     3001 cccacagcag ccccataagc gcactaaagc tcaatgcgat aaaccaccac gttttccaaa
     3061 aaggcggaac aatccgtatc ggaatcgaaa taccttgttc attccaaacg ccgtcattat
     3121 tggatccgcg tacattaaaa acataattcc ctgcatctaa gttagtataa ttcacataac
     3181 ggcgtgtacc cgcaaggatc cagtttttat caaatccttc taatttgtat gcatattgat
     3241 ttttggccgg ctctgtaaaa tcaagcgaag caaattcaaa tgagaagtct tcattgtaat
     3301 cgaggataat cggatcgtgg cggtacacag attcaatata ggcgtcaaca aatgctttat
     3361 ctgatttgtg cattgttttg aatgccgtta atacgatttg cgggatatgc ggattgtcca
     3421 tcgcaagttt gggataaaaa cggttaaaac cgttggtccc gccgaaaaat atttcgccac
     3481 tgcgactttt atagtaactg ccatatttga attcattatt ctgaagaccg tcactttcat
     3541 caaaattacg aaacatcagc ttttccgatt ggtcgttctc atcttggatc agacgtgcgc
     3601 gggacaaacc ttggaccgtg ctgatccaaa gattgccatg ttcgtccggc agtattgcat
     3661 atacgtagtt atccggaagc ccgtcttttt cgtaataatt ataaaacttc aaactaccgt
     3721 ccgtagtgcg tacgatacga ttgagccctg agctggcgcc gatccaaata tttttatcgc
     3781 tatcttcgga aatagcaaaa atcacatcac tgcctaatga ggaattactt tcccctcgcc
     3841 cggctatacg tttaaaggaa gcataacccg tgcgatcctg aaccgaatcc aaacgattga
     3901 gacctccgcc aaatgtgcca acccagattt tttcttccga atccatcagt atggaactga
     3961 cctcattatg actcagactc tgcgaacgat gaagtacatt gcgataatgg acaaatattt
     4021 ctttcccgtt ttctacgatc aatttatcca gaccgtcgcc gaaagttcca atccacaatt
     4081 gattatattt atcgctcaac agtacggata tgtcattacc cgcgatactc agcgaatcgc
     4141 ccggacgatg ttggtacacg gtaaaccgat ttgttcggcg atcaaattta tttaacccgc
     4201 caccttcggt acccagccag atcattccgt ttttatcttc tgcaatggtt cgcacttcat
     4261 tgctactcag acgacccggt ccggcgcgat atgccgtaaa acgatcggta ccggcatcat
     4321 aacgattgag tccgtcgtgc gtaccgatcc acaaaatccc atcactatcc tccc
//
LOCUS       DAOQNQ010000273         4374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_8829_length_4374_cov_4.651308, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000273 DAOQNQ010000000
VERSION     DAOQNQ010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8829_length_4374_cov_4.651308"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2310)
                     /locus_tag="PK754_13215"
     CDS             complement(<1..2310)
                     /locus_tag="PK754_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNB10509.1"
                     /translation="MNLRAWLMLCSLLISTHVVAQKKNAETKDPMPSSTFGGLKFRSV
                     GPALTSGRVVDFAVNPKNIFEYYVAVASGGVWKTTNAGVTYEPVFDGEGSYSIGCVAI
                     DPSNPNVVWVGSGENNNQRAVAYGDGIYKSVDGGKSWTNMGLKNSEHIGRIAIDPNNS
                     DIVYVAAYGPVWSAGGDRGIYKTTDGGKTWKAVLTVSENTGFNEVIMDPRNSNVLYAA
                     AHQRRRSVFTYIGGGPESALYKSTDGGANWRKINSGLPAVDLGRIGIAISPINPDVLY
                     AIVEAAEGKSGVFRSDNRGESWEKRGNNSSSGNYYQEIFCDPQNIDKVYYVDLFVMLS
                     LDGGKSFNPIGEKYKHVDNHALWINPANTGHMLVGCDGGIYETYDNGANWEYKWNLPV
                     TQFYKVATDNATPFYYIYGGTQDNNSIGGPSRTLSANGIINTDWFITNEGDGFESQID
                     PFDANIVYAQSQYGGLVRYDKKSGEVIDIKPVEGENESGLRWNWDAPLLVSRHKANRL
                     YFGANILFRSDDRGHSWKAISKDMSRGLDRNKLSVMGRVWSIDAVAKNGSTDIYGQLV
                     SIAESPLDENVLYTGTDDGLIHVSRDGGASWTVVDNIPGVPERTYVNQIIASQHDKGT
                     VYAIFNHHRYGDFKPYIFRSRDFGKTWSAIQNNLPERGSVYCVGEDHMRADLLFVGTE
                     FGVFFTIDGGQKWVQLKSGLPTIAVRDLEIQRRENDLVLGTFGRGFYVLDDYTPLRHL
                     KKEDLSKEAMLYPVKDALMFIESRPLGLSE"
     gene            complement(2359..>4374)
                     /locus_tag="PK754_13220"
     CDS             complement(2359..>4374)
                     /locus_tag="PK754_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HNB10510.1"
                     /translation="ADQGAIITVNGGKTYSSWYNQPTAQFYHVSTDNAFPYNVLGGQQ
                     ESGSASVSSRGHGGQITYRDWLPVGVEEWGYVAADPLNPDLVYGGKLTKYDRKTGHTQ
                     SVGPFPTGHYQPNRSPYRFLRTAPVIFSQADPTALYYAGNVLFKSQDGGHAWRIISPD
                     LSRESWDVPKSIGVYTTESMKTMARRGVIYTVAPSPLDSSVIWAGTDDGLIHITRDGG
                     KTWQNVTPAEITSWSKISLIDAGHFDVNTAYVAVNRIRLDDMKPYIYKTHDGGKTWIK
                     IVEGLPDDPINAVREDHIKEGLLICGSERAVYASVNDGQNWLSLRNNMPATSIRDIVI
                     KDNDIVAGTHGRSFWIMDDIMPLRHITEVSRQAPVWLFKPSNAYRVRWNLYTDTPLPQ
                     EEPAGENPPDGAIIYYHLKQDAQTPIVIEIKNSRGEVVRRFSSNDSIPMSQDNNFPSY
                     WLAPPQRVSAKAGSHRLIWDLRHNPLPTEPEFSIAAIYGKTTPAPTSPLVMPGFYSVV
                     ISVNGLTMGEGLTLVMDPRVRMTDTEIKDQFDLSMRCYEIRKKGMLVTQHIQSWRVQA
                     DIYMGQIKSNSSEIKKFLGYLDAIEKGNTATGDKGLQTILQSATNMFDVIQHADMPPT
                     AAMYNTVRETEKSWEKLTAEWEKLKTVYLNDLNAHLKKNKLPEISNP"
BASE COUNT         1139 a         1046 c          964 g         1225 t
ORIGIN      
        1 ttcgctaagt cccaacgggc gtgattcgat aaacatcaac gcatctttta caggataaag
       61 cattgcttct ttggaaaggt cttctttttt gagatgacga aggggcgtgt aatcatcgag
      121 tacatagaac ccacgaccga atgtaccgag gacaagatca ttttcgcgac gttgaatttc
      181 aagatcgcga acagctattg tcggtagccc tgatttgagt tgcacccatt tctgaccgcc
      241 atcaatcgta aaaaacacac caaattccgt tcctacgaat aacaaatcag cgcgcatatg
      301 atcctcacct acgcaataaa ctgatccgcg ttccggcaag ttgttttgaa tggctgacca
      361 cgtttttcca aaatcacggc tacggaagat atacggtttg aaatcaccgt aacgatgatg
      421 attgaaaatc gcgtataccg ttcctttatc atgttggctt gcgatgattt gattgacata
      481 tgtgcgttca ggaactccgg gaatattatc cacgacagtc caagatgcgc cgccgtcacg
      541 cgatacatgg ataagcccat catctgtgcc ggtataaaga acattctcgt ccaaaggcga
      601 ttcggcgata cttacgagct gaccgtaaat atccgttgag ccgtttttag ctacggcgtc
      661 aatactccaa acacgcccca tcacggataa cttattacga tccaaaccgc gactcatgtc
      721 tttgctaatc gctttccatg aatgaccacg gtcatcactt cggaaaagga tattggctcc
      781 gaaatacaaa cgattggcct tgtgacggga aacaagcagc ggagcatccc aattccaacg
      841 caatcccgat tcgttttctc cttcaaccgg tttgatgtca atgacttccc cgctcttttt
      901 atcgtagcgc acaagtccgc cgtactgcga ttgtgcgtac acgatattgg catcaaaagg
      961 atctatctgc gattcaaaac cgtcgccttc attggtgata aaccaatccg tgttgatgat
     1021 gccattggcg cttagtgtgc gcgaaggccc gccgatactg ttattgtctt gcgtgccgcc
     1081 gtagatataa taaaacggtg tggcgttgtc ggtggccact ttgtaaaact gagttacagg
     1141 aagattccat ttatattccc aatttgcacc gttatcatat gtttcgtata tgccgccgtc
     1201 gcaaccgaca agcatatggc ctgtattggc cggattgatc caaagcgcgt gattgtccac
     1261 gtgtttgtat ttttcgccga tcggattaaa agattttcct ccgtccaaag aaagcattac
     1321 aaataaatcc acataataaa ctttgtctat attttgtgga tcgcaaaaaa tttcctgata
     1381 ataatttccg gatgaggaat tatttcctcg tttttcccag ctttcaccac gattatcgga
     1441 acggaagaca ccgctttttc cttcagcggc ttcaacgatc gcatacaaca catcgggatt
     1501 aatcggtgaa atggcgattc cgatacgacc gagatctacc gcgggcaaac cggaattgat
     1561 tttccgccag ttggcaccgc cgtcagtaga tttgtacaac gcagactctg ggccgccgcc
     1621 gatgtaggtg aaaacggatc tgcggcgttg atgggctgcc gcatagagta cattggaatt
     1681 acgtggatcc atgatcactt cattgaaccc cgtgttttcg ctgaccgtga gaacggcttt
     1741 ccatgtttta ccgccgtccg tcgttttgta aataccgcgg tcaccgccgg cactccaaac
     1801 cgggccatat gccgctacgt aaacgatgtc tgaattattg ggatcaatgg cgatacggcc
     1861 aatatgttct gaatttttaa gacccatatt ggtccaggac tttcccccgt ccacagattt
     1921 gtatatacca tcgccgtaag ccaccgctct ttggttgtta ttttcaccgc tgccaaccca
     1981 taccacatta ggattggaag gatcaatcgc cacacagcct atggagtatg acccttcgcc
     2041 gtcaaaaaca ggttcatacg tcacaccggc attggttgtt ttccacacac cgccggatgc
     2101 gacagctacg taatattcga aaatattttt aggattgacg gcaaagtcta cgacacggcc
     2161 gcttgtcaga gccgggccga cactacggaa cttgagtcct ccaaaggttg aagaaggcat
     2221 ggggtcttta gtttcagcat ttttcttttg cgctacgacg tgcgtcgaaa tcagcaacga
     2281 acatagcatt aaccaagcgc gtaaattcat gatatttctc cgattttaaa tgaataatga
     2341 aaaataatca gattgctatt atgggtttga tatttcaggt aatttatttt ttttcaaatg
     2401 agcatttaag tcgttgaggt aaaccgtttt tagtttttcc cattcagccg ttaatttttc
     2461 ccaactcttt tccgtttcgc gaacggtatt gtacattgct gcggttggtg gcatatcggc
     2521 atgttgtatc acgtcaaaca tattggttgc tgattgcaaa atagtttgca accctttgtc
     2581 gccggtagcg gtgttacctt tttcgatagc atccaagtaa cctaaaaatt ttttaatttc
     2641 actcgaatta ctttttatct gccccatata tatatcagct tggacacgcc acgactgaat
     2701 gtgttgagta acaagcattc ctttttttct tatttcgtaa caccgcattg acagatcaaa
     2761 ttgatctttg atttccgtat ccgtcatccg tacacgcgga tccatgacga gggttaagcc
     2821 ctcacccatg gttaacccgt taactgaaat gactacggaa taaaaaccgg gcatgaccaa
     2881 tggcgatgtc ggcgcaggtg tagtcttacc ataaattgcg gcaatggaaa attccggctc
     2941 tgtcggcaat ggattatgac gaagatccca tatcaggcga tgtgatccgg ctttggcgga
     3001 aacacgctgc ggtggtgcaa gccagtatga tgggaaatta ttatcctgtg acatagggat
     3061 cgaatcattg ctcgaaaaac gtcgtacaac ttcaccacgc gaatttttaa tttcgatgac
     3121 tatcggggtt tgcgcatcct gctttagatg ataatagatg attgctccat ccggaggatt
     3181 ttcaccggcg ggctcctctt gcggaagcgg tgtatcggta tacagattcc aacgaacacg
     3241 gtacgcgtta gatggtttga aaagccatac cggtgcttga cgtgatactt cggtgatgtg
     3301 ccgaagcggc atgatgtcat ccataatcca aaatgaacgc ccgtgtgtac ctgcaacgat
     3361 gtcgttatct ttgattacaa tatcacgaat ggaagtggca ggcatgttat ttctaaggct
     3421 aagccaattt tggccatcat tgacagaagc atagactgcc cgttcagacc cgcatataag
     3481 caaaccttct ttgatatgat cttcgcgaac ggcgttgatt gggtcatcgg gcaatccttc
     3541 gactattttg atccatgttt ttccaccgtc gtgcgtttta taaatgtacg gcttcatatc
     3601 atccaaacga atgcgattga ccgcaacgta ggctgtattg acatcaaaat gacccgcatc
     3661 tataagtgaa atcttgctcc atgaggtaat ttcagctggc gttacgtttt gccatgtttt
     3721 accgccatcc ctggttatat ggattaaacc gtcgtccgta ccggcccata tcaccgatga
     3781 gtccaaaggc gaaggtgcga cagtatagat cacaccgcga cgcgccatgg ttttcataga
     3841 ttcagtagta tatacaccaa tgctttttgg aacatcccat gactcacgcg aaagatcggg
     3901 actgataatc cgccaagcgt gtccgccatc ctgtgatttg aataatacat tacctgcata
     3961 atataaagcc gtaggatccg cttgagaaaa aataaccgga gccgttctca aaaaacgata
     4021 cgggctgcga ttgggttgat aatggcctgt cggaaaagga ccgacggatt gggtatgacc
     4081 ggttttacga tcgtattttg ttaattttcc gccatacacc aagtccgggt taagaggatc
     4141 agcggctaca tatccccact cttcgacgcc gacgggcaac caatcgcggt acgttatttg
     4201 tccgccatga ccgcgtgaac ttacgctggc ggatccgctt tcctgttgac ccccgagaac
     4261 gttgtatgga aatgcgttat ccgttgacac atgataaaac tgagccgtcg gctgattgta
     4321 ccacgatgaa taagtttttc ctccattaac agtaatgatc gcgccttgat cggc
//
LOCUS       DAOQNQ010000274         4355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_8889_length_4355_cov_5.811860, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000274 DAOQNQ010000000
VERSION     DAOQNQ010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8889_length_4355_cov_5.811860"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..2274)
                     /locus_tag="PK754_13225"
     CDS             complement(79..2274)
                     /locus_tag="PK754_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10511.1"
                     /translation="MFSFWLSNKLRGSMLILRIIAPAMMAFIPLTYDLDYEFAEALTI
                     INVIFFAVWMSTERAHSHINYRYGFLRTVNRLLLPIIFFDLVIISIAMIAGHHLHWYR
                     TFGLYFLLPFVGSWIGLTLGYVCRFFIQRRPWLWVATILLSTLILNLIWLLFTAPIFV
                     YSIWWGYFAGPIYDEWIPLTDTLLFHRLFSLGLAFAIFISGDLITRKLNNTITYKKFV
                     FWITTVWLIITGIFYWRDNWGWDTSYATLEQKLNVVYQDERLTIYSDTTSKFITRYVD
                     ARIKQVTDWLNINPAKVKIFIYPDAQTKKILMGAGGTNFAKVWRNEVHVNSDDAEFTI
                     KHEAVHVLASSFGHPLYSTTKPGMLEGLAVAAAWDESQLTPHEWSAALYKLNKLPDIT
                     ALMEPMGFWSSSSGLAYTVSGSFVRFLIDQYPIQNIHRVYASEDFKDVYGLPLDTLRE
                     RWKFFLNTIELDSLDLRIATTLLQPSLFRRRCPHYVADLQKEYYASIEMKEFDGALKL
                     TEQIISTGTSPERWVLSRARIHFKQKKYDAALSKVEEVLNRSTLSYTMRGWALLLKAD
                     IVFCQGQIQKAMDLTHEIMSLYDQTTSVYEAALWSDHLMRHPGNGTLYASWLDAVDQN
                     KVGEWYSVHYDKKDDVIFSYKLVSYLYQTSQYDSIFSYIKTHPMAITDSSVYLKYNQL
                     LGDAAQRTELWSEAEQYYRVGLQYAKRTTEKVQFDDRLELIHYLKNKIE"
     gene            complement(2285..3157)
                     /locus_tag="PK754_13230"
     CDS             complement(2285..3157)
                     /locus_tag="PK754_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10512.1"
                     /translation="MISNNRFVVLVCILWLISSLGCDKNIKIIRDGFRLEYAINNPAH
                     AFHVRLVFETNPRDSLIGLMYIARTAKESGILDYREGHTLRQATGDTLFMNPSQFSDL
                     LECVNDLHKMIKPSNVYGQSDGKIVNINLRHSGKSYDWRLFGKTDQSSVKKLTAYIET
                     MFEETYPGISLSAENSRIELSLCNHANSKNTETNFSIYSNNNIIRVHQDSLSAYTLDA
                     GKHLYLWQVLEANDVWRLDDNKTMQGKYPSFYELKIQMGKRTHRWSAYAPIYLDDRRY
                     LRIVQAIESTVLSR"
     gene            complement(3154..>4355)
                     /locus_tag="PK754_13235"
     CDS             complement(3154..>4355)
                     /locus_tag="PK754_13235"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNB10513.1"
                     /translation="SFGWATLATILLLITWSGGFEFSRMARSLSEKGPASLIMNLNVP
                     DAEIMIDGDRYTPQSSLFRLDSVGVGERRITVKRDHYESYETAVIFGKGEQKNIEAHL
                     KPLPVEIPPGSDTSFVTIRSSPDMVKVETSYGKFLGFTPIDSLIFPGGRYTLIFSRED
                     FISRKRDIALGKKRLTYVEQSLEKQRGTVSLDQVFPEQSYLMVNGRRIQKNYRNNSYV
                     LPVGNQVLTIKADGYDDLVKSLRVEPDSVLVLTDSLKAQWTSVRFESNPTGADVYLDG
                     SEESVGKTPVIVNDLLANSHKLRAVLRNEKRLMNFRAVKGDTATVRVVFANPNGYLNL
                     VSDPPGAEIFLNTVRDDDYKTPVKRELKPGFYKVRLTHPSFKKYHEITVRVRPDFTTE
                     LVHKFEP"
BASE COUNT         1283 a          963 c          875 g         1234 t
ORIGIN      
        1 cgcattaaga gtgtttatgc aaacactgta aatcaaaaaa aacagggata aaactaattt
       61 catcaaaagt tgcatgcgtt attcaatttt atttttcaag taatgaatca attcaaggcg
      121 atcatcaaac tgaacttttt cagttgttcg tttggcgtac tgcagcccca cgcgataata
      181 ttgctcggct tcgctccata gctcggttcg ttgtgcggca tcacccaata actgattata
      241 ttttagatat accgagctat cggtaatagc catagggtgt gttttgatat acgaaaaaat
      301 agaatcatat tgcgaagttt gatataaata ggaaaccaat ttatacgaaa aaatcacatc
      361 atcctttttg tcgtaatgca ctgaatacca ttcgccgact ttattttgat caacggcgtc
      421 cagccatgaa gcgtacagag tgccgttacc cggatgtcgc atcaagtgat cggaccataa
      481 cgccgcttcg tatacggatg tcgtttggtc atagagcgac ataatctcat gcgtcaaatc
      541 catagctttt tggatttgac cttggcaaaa tacaatatct gcttttaata ataaagccca
      601 tcctcgcata gtataggaaa gcgtacttct atttaatacc tcttcgactt tcgatagcgc
      661 ggcatcatat ttcttttgct taaaatggat acgtgcacgg ctcaataccc agcgttcggg
      721 cgacgtccca gttgatataa tctgttctgt aagcttaagt gcgccatcaa attctttcat
      781 ctcaattgac gcgtaatatt ctttttgcaa atcagcaaca taatgcgggc accgtcgtct
      841 gaataatgaa ggctgtaata gcgtggtcgc aatgcgaagg tcaagcgaat caagttcgat
      901 cgtatttaaa aaaaacttcc atcgttcccg gagcgtgtcc aatggcagtc cgtaaacatc
      961 tttaaagtct tccgacgcat acacgcggtg tatattttgg atgggatatt gatctatgag
     1021 aaaacgtaca aatgagcctg aaacggtata tgccaaaccc gatgaactgc tccaaaaacc
     1081 catcggttcc atcaaggccg taatgtccgg cagtttgttt aatttataga gtgcagcgga
     1141 ccattcatgc ggtgttaatt gcgattcatc ccacgccgcc gctacagcga gtccttccag
     1201 cataccgggt ttggtagtac tataaagcgg atgcccaaac gaagacgcca gtacgtgtac
     1261 cgcctcatgt tttattgtga attcagcatc atcagaatta acgtggacct cattacgcca
     1321 tactttggcg aaatttgttc ccccggcacc catgagtatt ttttttgttt gcgcatcggg
     1381 atagatgaat attttaactt tagcgggatt gatattaagc caatccgtga cctgttttat
     1441 cctcgcgtcg acataacgtg ttataaattt agaagtcgta tcactataaa ttgtgagtcg
     1501 ttcatcttgg tacaccacat tcaatttttg ttcgagggtt gcataggatg tgtcccagcc
     1561 ccagttatcg cgccagtaaa atatgcctgt aatgatcagc cataccgtag ttatccaaaa
     1621 aacaaatttc ttgtaagtaa tagtgttatt caattttcgt gtgattaaat caccggatat
     1681 aaaaatggca aatgctaacc ccaatgagaa taaacgatga aataaaagcg tatctgtcaa
     1741 tggaatccat tcgtcgtaga tcggacctgc aaaatagccc caccaaatgc tataaacgaa
     1801 aatcggtgcg gtgaaaagca accaaatcag atttaggatc aaggtggata acaggattgt
     1861 cgccacccac aaccaaggcc ttctttggat aaaaaagcga cagacatatc caagggtaag
     1921 acctatccaa cttcctacaa agggaagcaa aaaatacaaa ccaaaagtac gataccaatg
     1981 caaatgatga ccggcaatca tcgcaatcga aatgatgaca agatcaaaaa aaataatcgg
     2041 caacaacaaa cgattaacgg ttcgtaaaaa accgtaacga tagtttatat gggaatgcgc
     2101 cctttccgtg ctcatccaaa cggcgaagaa aatgacatta attatggtta atgcctcagc
     2161 aaattcataa tccaaatcgt atgttaaggg tataaatgcc atcatcgccg gcgcgatgat
     2221 ccgtagtatt agcattgacc cgcgaagttt attggatagc caaaagctaa acatgtattt
     2281 ccttttaacg gctcagcacg gttgattcga tagcttgtac gatgcgcaga taacgacgat
     2341 catcaagata gatcggcgcg taagcagacc aacggtgtgt tcgcttgccc atctgaatct
     2401 tcaactcgta aaagctcgga tatttaccct gcatggtttt attatcatca agacgccaga
     2461 catcgtttgc ttccaaaacc tgccaaaggt acaaatgttt tccagcatct aatgtgtatg
     2521 cactcagact atcttgatgt acacggatta tattattgtt cgaatagatc gaaaaatttg
     2581 tttctgtgtt ttttgagttt gcatgattgc agagtgacaa ttcaatgcgc gagttttcag
     2641 ccgacaaaga aatgccggga tacgtctctt caaacatcgt ttctatataa gcagtcagct
     2701 ttttgaccga agattgatcc gttttaccaa atagccgcca atcataactc tttcccgaat
     2761 gccttagatt gatattgact atttttccgt cggattgtcc atacacgttg gatggtttta
     2821 tcattttgtg taaatcattc acacactcta atagatcaga gaactgagac ggattcatga
     2881 ataatgtatc accggtggct tgacgcagcg tatgcccttc gcggtaatcc aaaatacctg
     2941 attcttttgc tgttctggca atatacatca ggcctatcaa actgtcgcgg ggattggttt
     3001 caaaaacaag acgtacatgg aaggcatgag cgggattgtt gatcgcgtat tccaaccgaa
     3061 acccgtcgcg aataattttg atgtttttat cgcatcccaa actcgatata agccatagaa
     3121 tgcaaactaa aacgacgaat cggttattgg aaatcacggt tcaaacttat gtacaagctc
     3181 ggtcgtaaag tcaggacgga cccgtaccgt aatttcgtga tattttttaa atgacgggtg
     3241 cgtcaatcgt actttataaa aaccgggttt caattcgcgc ttgaccggcg ttttgtagtc
     3301 atcgtcgcgt acggtattaa gaaatatttc ggcgccgggc ggatcgctta ccagattgag
     3361 atacccgttc gggtttgcaa aaacaacacg cacagtagcc gtatcgcctt tgacagcgcg
     3421 gaaattcatt agccgttttt cattacgcaa taccgctcgt aatttatgcg aattagcaag
     3481 caaatcattt acaataaccg gtgttttacc taccgattct tccgatccgt ctaagtacac
     3541 gtccgcgcct gtgggattac tttcaaaacg cacggatgtc cattgcgctt tgagggaatc
     3601 cgtcagtaca agcaccgaat ccggttccac acgcaaagac ttgactaagt cgtcataacc
     3661 atcggcttta attgttagta cttgatttcc gaccggcaag acatatgaat tatttctata
     3721 atttttctga attcgtcttc cgttcaccat aagatagctt tgttccggaa aaacctgatc
     3781 cagtgaaacg gtaccgcgtt gtttttctaa tgattgctcc acatacgtca gacgtttttt
     3841 tccaagtgca atgtcgcgtt tacgcgaaat aaaatcctca cgtgaaaaaa tcaacgtata
     3901 gcgacctccg gggaaaatca aactgtctat cggcgtaaat cctaaaaact taccgtagct
     3961 tgtttccact ttgaccatat ccggcgatga gcgaatagta acaaacgaag tatcgcttcc
     4021 cggcggtatt tcgaccggta acggtttaag atgcgcttct atgttttttt gttccccttt
     4081 tccgaaaatc accgcggttt cgtatgattc gtaatgatcg cgctttaccg taatgcggcg
     4141 ttcaccgacc cccacgctat cgagacgaaa caatgaactt tgcggggtat aacgatcacc
     4201 gtcaatcata atttcggcat cgggtacatt gagattcatg atcaatgaag cgggaccttt
     4261 ttcgctcaaa gaacgcgcca tccgactaaa ttcaaatccg ccggaccatg tgataagcag
     4321 taaaatcgta gctaatgtcg cccaaccgaa cgata
//
LOCUS       DAOQNQ010000275         4353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_8897_length_4353_cov_5.027455, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000275 DAOQNQ010000000
VERSION     DAOQNQ010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8897_length_4353_cov_5.027455"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..367)
                     /locus_tag="PK754_13240"
     CDS             complement(41..367)
                     /locus_tag="PK754_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10514.1"
                     /translation="MRQRLTQLGIIVSVFVLFLSGFDALDQQRYAMASLYLLCVALYI
                     AVWRISLRVVMQKQVWLTWVGTFVILIAAMDYFSQGKKYLPYAYILAAVINTIPVFFK
                     RRSAKS"
     gene            complement(372..1472)
                     /gene="rho"
                     /locus_tag="PK754_13245"
     CDS             complement(372..1472)
                     /gene="rho"
                     /locus_tag="PK754_13245"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNB10515.1"
                     /translation="MSEVFSGILETDPKGFGFIRTLSLTLSPSPKDVFVPPGIITQNR
                     LRPGVLVEGTGAPGDKGNTRVATITRINGMEPEAWKSVQDFGTLQVIQPRDRISLTGR
                     EQNTSMRVVDLFCPIGRGQRGLIVAPPKAGKTRLLQELAHAISMHNDDITMIVLLIDE
                     RPEEVTDMRRSIRGEVFASSSDSAGAQHIRLAQLVLEYAKRKVEAGKHIVLLLDSLTR
                     MGRAFNVQQKSSGRTLSGGVDIRALEIPKRIFGAARNIEHGGSLTILATALIETNSRM
                     DELIFQEFKGTGNMELVLSRELSDERIFPAIDLNKSGTRNEELLFGNDTMRHQTLRRS
                     LARIQPREAMKQVLELIQRFPDNALLLDQVKG"
     gene            1664..2323
                     /locus_tag="PK754_13250"
     CDS             1664..2323
                     /locus_tag="PK754_13250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HNB10516.1"
                     /translation="METGTILGFLKKLKRNNKKTWFDAHRDDYAMAKMSFENFVGDLI
                     GDMTVVDPSLASLQPKDCIFRIFRDTRFSKDKTPYKTNFGAFLASGGRKSSGAGYYIH
                     IEPGASMLGGGLYMPPSDILLKMRNYVAEEADGLKKIIKAPAFKKYFNELQPEKLKTM
                     PKGFPASHPHAEWLKYTSYVVLHKISDKELSNPRFEEHVIKGFKAMKPLCDFINDAVS
                     Q"
     gene            2418..3377
                     /locus_tag="PK754_13255"
     CDS             2418..3377
                     /locus_tag="PK754_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB10517.1"
                     /translation="MGSSMDFREKILMIDDEPDKIFIFSTILQNKGFEVITSESGREG
                     LEIMTRIRPDLILLDINMPDINGHTVREKLKERPSTRNVPIVMFSSSDQLPDKLASLR
                     NGADDYITKDVDHQELTARLEALIRRYKESIGANPLTKLPGNYAIEDDINFRIAQNQK
                     QKTPFAVCYADLDNFKAYNDKYGFKSGDEAIQGTARIILDVIGELGAKEDFVGHVGGD
                     DFVFIVSRYDRVEAICKTIIERLDDFYPSLYDETDRKAGHIITKNRKDEIDQFPLMSI
                     SIAVVSNENRELSSMGQIAQIAAEVKKAVKKKDGSNYLIDKRS"
     gene            3441..>4353
                     /locus_tag="PK754_13260"
     CDS             3441..>4353
                     /locus_tag="PK754_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNB10518.1"
                     /translation="MFKKILIANRGEIALRVIRACREMGIKTVAVYSEADANSLHVRF
                     ADEAICVGPAPSAKSYLNIPQIMSAAEITGADGIHPGYGFLSENIKFAEICESVGVKF
                     IGPKSHMIQKMGDKAFAKDTMKKAGVPVVPGSDGIVRDIKHAREIMKEAGLPVIIKAT
                     AGGGGRGMRIVREESEFENAYNTARAEAESAFGNPDVYIEKFIEKPRHIEIQLIGDQH
                     GNVFHIGERDCSMQRRQQKLIEESPSPAVDEALRQKIGEAAVRGAKSVNYESVGTIEF
                     LMDKEKNFYFMEMNTRIQVEHCVTEMVY"
BASE COUNT         1255 a          994 c         1015 g         1089 t
ORIGIN      
        1 tatcagcatc ggtgaagctt tttgataaca gatgcgcgga ttaggatttg gccgaccggc
       61 gtttaaaaaa taccgggatc gtattgatta cagcggctaa aatataagca taaggcagat
      121 atttttttcc ttgggaaaaa taatccatcg ccgcaatgag gataacaaac gtacctaccc
      181 atgtcagcca cacttgtttt tgcatcacca cacgaagact gatccgccag acggcaatat
      241 acagcgccac gcataacaga tatagcgacg ccatcgcgta acgctgctga tctaaggcat
      301 caaatcccga aagaaaaaga acaaaaacac tgacgatgat tcccaattgt gtcagacgct
      361 ggcgcatatg attatccttt cacctgatcc aataacaacg cattatcagg aaatcgctga
      421 ataagttcaa gaacctgctt catcgcttcg cgcggttgta tgcgggcgag tgacctccgc
      481 aatgtttgat gccgcatcgt atcgttgcca aacaaaagct cttcattacg cgtacccgat
      541 ttattcaaat caatcgcagg aaaaatacgt tcgtccgaaa gctcgcgcga aagcacaagc
      601 tccatattac cggtcccttt gaattcctga aagatcaatt catccatacg gctattggtt
      661 tcgatcaatg ccgttgcaag aatcgtcagc gatccgccgt gttcgatatt gcgcgccgct
      721 ccgaaaatac gtttgggaat ctccagtgcg cgtatgtcaa caccgccgga cagcgtccgt
      781 ccgctgcttt tttgctgtac gttgaatgcc cgacccatgc gtgtaagtga atccaataaa
      841 agaacaatat gtttgccggc ttcgacttta cgcttggcgt attcgagcac gagttgtgca
      901 aggcgaatat gttgcgcccc tgcgctgtcg ctgctgctgg caaaaacctc accgcgtata
      961 gacctgcgca tgtcggtcac ttcttcaggg cgttcatcaa tcagcaaaac gatcatagtg
     1021 atgtcgtcgt tatgcatcga aatggcatgc gccaattcct gaagcaatct tgtcttacct
     1081 gctttgggcg gcgctacgat caatcctctc tgaccgcgtc cgatcgggca aaaaaggtcc
     1141 accacacgca tggaggtgtt ttgttcgcgc cccgtcagac taatccgatc cctgggttgg
     1201 atcacttgca aagttccgaa atcctgcacc gatttccatg cttcgggttc catgccgttg
     1261 atacgcgtaa tcgtagcgac acgtgtattg cctttgtcgc cgggcgctcc tgttccttcg
     1321 acgagaacac cggggcgcag acggttttgt gtgattattc cgggaggcac aaaaacatct
     1381 ttcggtgacg gcgatagcgt caaactgagc gtgcgaataa atccaaaacc tttgggatcc
     1441 gtttccaata tcccggagaa tacttcagac ataacaatgt aattgggtaa taatgataga
     1501 attcaatttg aaatcaaaaa gacaaaaaca tgatatgcac cggcacgttt ataatcaagc
     1561 ggtttattaa tctattttaa aaacaatgga atatagtttt ttcacaaact actttacgca
     1621 taccgtttta tttcatccaa ctatctaagc gaggaggtct tctgtggaaa ccggcacgat
     1681 tctcggcttt ttaaaaaaac tgaaaaggaa taacaaaaaa acatggtttg atgcccatcg
     1741 cgatgattat gctatggcca aaatgtcgtt tgaaaatttc gtaggcgatc tgatcggcga
     1801 tatgactgta gtggatccgt cgttggcttc tttgcaaccc aaagactgta tattcagaat
     1861 attccgtgat acacgttttt ccaaagacaa aacaccctac aaaaccaatt ttggcgcttt
     1921 ccttgcatca ggcggacgta aatccagcgg agcgggatat tatattcaca tcgaacccgg
     1981 cgcctccatg ctgggtgggg gtttgtacat gccgccatcg gatattcttc tcaaaatgcg
     2041 taactatgtg gcggaagaag ccgacggctt gaaaaaaatc atcaaggcac cggcattcaa
     2101 aaaatatttt aacgaactcc aaccggaaaa gctgaaaacc atgcccaaag ggtttccggc
     2161 ttcgcacccg catgccgaat ggctcaaata cacgagctat gtggtgttgc ataaaatatc
     2221 cgataaagag ctttccaacc cgcgttttga agaacatgtg atcaaagggt ttaaagccat
     2281 gaagcctttg tgtgatttta tcaatgacgc cgtgtcccaa taatgcgttt tttgagcatt
     2341 atgcggttga actccttgat tgactttcgt ctgtggtttt gataatttcg ctcgttttca
     2401 tcggttaacg gattactatg ggaagcagta tggattttcg cgaaaagata ttgatgattg
     2461 atgatgaacc ggataaaatt tttatctttt cgacgatact ccaaaacaaa ggttttgaag
     2521 tgatcacctc cgaaagcgga cgcgaaggct tggaaatcat gacccgcata cggccggatt
     2581 tgattctttt ggatatcaat atgccggaca tcaacgggca caccgttcgc gaaaaactca
     2641 aggagcgtcc gtccacacgc aatgtcccga ttgtgatgtt tagctccagc gatcagttgc
     2701 ccgacaaatt agcatcctta cgaaacggag ccgatgacta tatcaccaaa gacgtggatc
     2761 atcaggaatt aacggcccgt ttagaagctt tgatccgtcg ttataaagaa agcatcggcg
     2821 caaatcccct gaccaaatta ccgggaaatt atgcgatcga agacgacatc aattttagaa
     2881 tagcacaaaa tcaaaaacaa aaaacacctt ttgccgtttg ttatgcggat ttggataatt
     2941 tcaaagcata caacgataaa tacggtttca aaagcggaga cgaggccatt cagggtacgg
     3001 cgcgcataat cctcgatgtg atcggcgaac tgggcgcaaa agaagatttt gtcggccatg
     3061 ttggcgggga tgacttcgtc ttcatcgttt cgcgttacga tcgagtggaa gcgatttgta
     3121 aaacgattat cgaacgatta gatgattttt atccgtccct gtatgacgaa acagaccgta
     3181 aggcgggtca tattataact aaaaacagaa aagacgaaat agatcaattt ccgcttatgt
     3241 ctatttcgat tgccgtggta agcaacgaaa accgcgagtt gagtagtatg ggacagatcg
     3301 cccaaatcgc tgccgaagtc aaaaaagcgg tgaagaaaaa ggacggtagt aattatctga
     3361 ttgataaacg atcataatac gtattatata acacatgcgg gcgcttgtac gccggcatgt
     3421 atcattaacc ggaaggaagt atgttcaaaa aaattctgat cgccaatcgc ggtgaaatag
     3481 cgttacgcgt aattcgcgca tgccgtgaga tgggtatcaa aacggtggcc gtatattccg
     3541 aagcggatgc caattcactc cacgtgcgtt ttgccgatga agccatttgc gtgggcccgg
     3601 cgccaagtgc caaaagttat ctgaatattc cacagatcat gagcgccgca gaaataaccg
     3661 gtgccgacgg cattcacccg ggttacgggt ttttatctga gaacatcaag tttgcggaaa
     3721 tctgtgaatc ggtcggtgtc aaatttatcg gccccaaatc tcacatgatt caaaaaatgg
     3781 gcgataaggc ttttgctaaa gatacgatga aaaaagccgg cgtaccggtt gtccccggaa
     3841 gcgacggtat cgtgcgggat atcaaacatg cccgcgaaat catgaaagaa gccgggcttc
     3901 ccgtgatcat caaagccaca gccggcggcg gcggacgcgg aatgcgtatt gttcgcgagg
     3961 aatcggagtt tgaaaatgcg tataacacgg cacgggcgga agccgaatcg gcatttggga
     4021 acccggatgt ttacatcgaa aaattcattg aaaaaccgcg ccacatcgaa atccagctca
     4081 tcggcgatca gcatggtaac gtatttcata tcggcgaacg cgactgttcg atgcaacgtc
     4141 gtcaacagaa actgatcgaa gaatcccctt cgcccgcggt ggatgaagct ttgcgtcaaa
     4201 aaataggtga ggccgctgta cgtggcgcaa aaagcgtaaa ctacgaaagc gtgggcacta
     4261 tcgaattttt gatggataaa gaaaagaatt tttactttat ggaaatgaat acgcgtattc
     4321 aggtcgagca ttgcgtcacg gaaatggtat acg
//
LOCUS       DAOQNQ010000276         4313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_9022_length_4313_cov_5.631282, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000276 DAOQNQ010000000
VERSION     DAOQNQ010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9022_length_4313_cov_5.631282"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PK754_13265"
     CDS             <1..526
                     /locus_tag="PK754_13265"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNB10519.1"
                     /translation="VTETFEKIPIGQSEFVQRGKDIALLAVGNMVQHALSAAQELIRN
                     GYSATVVNIRFVKPLDEAMLFEVCRDFDDVITIEDNVTTGGFGSGVLETIYSSDFIER
                     GQKQDKDFVQKMARLRLKRLGLPDAIVEHGDNDVLYERVGLDPASIARDAKTMIEKRR
                     GGIQVETIMAAAAR"
     gene            complement(596..1573)
                     /gene="pta"
                     /locus_tag="PK754_13270"
     CDS             complement(596..1573)
                     /gene="pta"
                     /locus_tag="PK754_13270"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HNB10520.1"
                     /translation="MSIIASIRERARHKKCRIVLPEGEEDRTLLAAKTIINEQIAKPI
                     LLGDADKIRNRARELQVDLPSSVSIIDPKRSEKTADYASELFNLRKAKGMTYEQAKQH
                     MHSVLYYGAMMVRRSECEGFVGGAVSTTADVLRAAIHCVGTAPDINTVSSIFLMVLPD
                     GRAITYGDCAVMPYPDAAQLADIAIASAESHKQLTGEEPMVAMLSFSTKGSAKHEAVD
                     KVVQAHSIVREKRPDIKIDGELQFDAAFIESIGQRKAPGSAVAGKANVFIFPNLDAGN
                     ICYKVTERIGKAQAIGPVIQGLAKPINDLSRGCTPEDIANVTAICALKA"
     gene            complement(1639..2532)
                     /locus_tag="PK754_13275"
     CDS             complement(1639..2532)
                     /locus_tag="PK754_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10521.1"
                     /translation="MFRNSILSLTFIGLATMIFTGCPDEVNPPEPRPSKVRIKAHNGD
                     TLAVDRGTRPIEGALQLLRLEWSPVTDSDNLITEYIVYRSGGLDSAFAEIAVRTVNEA
                     ETDSFIEDAVIAEKTYYYYVVAKDSKGNTTDISDYLDEENLYTYVMPFRLAQKASPRR
                     PYNQDTVVTTKPQFSWCIFGDEPGYYVIKVATPSGQLVWLAYQQPGTFGENCGAQESM
                     TLNKQTYSYIDEFFDGIPGDSLYATDSPTRVVYANPNFFEGARLQKGVYQWRVDSYFG
                     KNNASRSPWVSFTVNRDYKNP"
     gene            complement(2549..3598)
                     /locus_tag="PK754_13280"
     CDS             complement(2549..3598)
                     /locus_tag="PK754_13280"
                     /inference="COORDINATES: protein motif:HMM:NF015634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0164 family protein"
                     /protein_id="HNB10522.1"
                     /translation="MKKSVRTLILLFISAATVITAQNRRYANAFLEIPAGVRAASMGS
                     AYSAIANDATAFFWNPAGSVLVNRHEVTGSYSSQFGGLAQYHVLGYNHQINNRYSFAV
                     GWIRYSISNIPENADLSGSASEHAVPGFDFAALQHGHFSYADNAFFFTFARMNTLRLN
                     LGWAYSDFPIQIPVGASFKIIRGGTSGISGENGVVLNDVRKSGIGVDIGTMIMFGMND
                     LLEIPTLGDFVMSFNLQDATTTAVQYNKISSDVRAQDIVKANFKFGMSYIQGLDEWKS
                     NILLSYESNTRYANDRHYGLEWEYNKLAALRLGIDNGTLTYGAGFQYNHIHIDYALNN
                     HALGYTHRIGTSYKF"
     gene            complement(3608..>4313)
                     /locus_tag="PK754_13285"
     CDS             complement(3608..>4313)
                     /locus_tag="PK754_13285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB10523.1"
                     /translation="TSISDRAPWMLMINRDRTAPVIETTIEGQLFTQGGIAPRQPRVS
                     AIVYDRNGVSLDRNHYAITVDNAPISASQIVLPDSVPNSNTVTFTLNLNQAFDDGQHE
                     VLFTAKDVNGNTSKTDTLNFQVIRKFDIESYGNYPNPFINTTTFAFRVEAPEPLEALD
                     ISIYTVSGRRIRKITDADAIASGKNITAVGYHEVTWDALDEDGRPIANGVYFYRIRGK
                     LNGKTIEKKGKLAYFR"
BASE COUNT         1152 a          969 c         1030 g         1162 t
ORIGIN      
        1 ggttacggaa acatttgaaa aaattccgat cggtcaatcg gaatttgtac aacgcggcaa
       61 ggatatcgct ttattggccg taggaaacat ggtccaacat gctcttagtg cggcgcagga
      121 gttaattcgc aacggttatt cggctacagt ggtcaatata cgatttgtca aaccattgga
      181 tgaagcgatg ctttttgaag tatgccgtga ttttgatgat gtgatcacga tcgaagataa
      241 tgttacaacc ggtggttttg gcagcggtgt attggaaacg atatatagtt ccgattttat
      301 tgagcgcggc caaaaacaag ataaagattt tgtacaaaag atggcccgtt tgcgccttaa
      361 gcgcctgggc ttaccggatg cgatcgtgga gcatggcgat aatgatgtat tatacgaacg
      421 cgtaggactt gatccggctt ctattgctcg ggatgctaaa actatgatcg aaaaacgtcg
      481 cgggggaatt caggtcgaaa ctattatggc cgcggcagca cgatagaatt aaaaaaaata
      541 caaatagaaa accctgaagt tttttctgca gggttttttt gttttagtat acgttttacg
      601 ctttgagcgc gcagatggcc gttacattgg caatgtcttc cggcgtacaa cctcgggaaa
      661 gatcattgat cggtttggct aaaccttgaa taaccgggcc gatggcttgt gccttaccga
      721 tgcgctcggt tactttatag caaatattac ctgcatcaag attaggaaaa atgaatacgt
      781 tcgctttacc ggcaacggcg ctaccgggag ctttgcgctg gccgatggat tcgataaatg
      841 ctgcatcaaa ctgcaattct ccgtcaattt ttatatccgg tcttttttcg cggacgatag
      901 aatgcgcctg aactacttta tccacggctt catgtttggc gctacctttt gttgaaaaag
      961 aaagcatcgc caccatcggt tcttcaccgg tgagttgttt gtgactttcg gcggatgcga
     1021 tggcgatatc cgccagttga gcggcatccg gataaggcat gacggcgcag tcgccgtacg
     1081 taatggcccg tccatccgga agtaccatca aaaaaatact cgaaaccgta ttgatatcgg
     1141 gcgccgtgcc cacgcaatgt atggccgcac gcaacacatc cgcggttgtg gatacggcac
     1201 ctccgacaaa accttcacat tcgctacgcc gtaccatcat cgcgccataa tataaaacag
     1261 aatgcatgtg ttgtttggcc tgctcgtaag tcatgccttt ggccttgcgg agattaaata
     1321 attccgaagc ataatccgcc gttttttcag aacgcttggg atcaatgatc gaaaccgatg
     1381 agggcaagtc cacctgcaat tcccgtgctc tatttcttat cttgtcggca tcaccgagta
     1441 aaataggttt agcaatctgt tcgtttatta tggttttggc ggcgagcaat gtgcgatctt
     1501 cttcgccttc gggaagaaca atacggcatt ttttgtgacg ggcacgttcg cgtatcgaag
     1561 caatgatgga cataaaaaaa tcctttagtt agtaaaacaa actaaaggat tttgcgatta
     1621 aaatcaacgc gtgcgctttt aaggattttt ataatcgcga tttacggtaa aggacaccca
     1681 cggcgatcgg gaagcattgt ttttaccgaa ataactatcc acgcgccatt ggtaaacccc
     1741 tttttgaagc cgagcgccct caaagaaatt tggattggca tatacgacgc gggtcgggga
     1801 atccgtagcg tagagtgaat cgcccggaat accgtcaaaa aattcatcaa tataagaata
     1861 ggtttgtttg ttgagcgtca tggattcttg cgcgccgcaa ttttcaccaa acgttccggg
     1921 ctgctgataa gcaagccata ccagttgacc ggaaggcgtg gcgactttga tcacataata
     1981 cccgggttca tcgccaaaaa tacaccagga gaattgcggt ttggtggtca ccaccgtatc
     2041 ctgattatac ggacgtctcg gggaggcttt ttgtgcaaga cggaaaggca tgacatatgt
     2101 gtacagattt tcttcatcta gataatcgga tatatcggtg gtattacctt tagaatcttt
     2161 tgcgacgaca tagtagtaat acgttttttc ggcgatcacg gcgtcttcga taaacgaatc
     2221 ggtttcggct tcatttacag tacgaacggc gatctctgca aaggcgctgt ctaaaccgcc
     2281 ggatcggtac acgatatatt ccgtgataag attatcagag tcggtgaccg gtgaccattc
     2341 taaacgaagc aattgtaaag cgccttcgat cggacgtgta ccgcgatcaa ccgccagcgt
     2401 atcgccgtta tgtgctttga tacgcacttt ggaaggacgc ggctccggcg gattgacttc
     2461 gtccggacat ccggtaaata tcattgtagc caagccgata aatgtaagcg ataaaatgct
     2521 gttgcgaaac atgatcatcc tttatgaact aaaacttata cgaagtaccg atacgatgcg
     2581 tgtatcccaa cgcgtgatta ttcaatgcgt aatcaatgtg gatgtgattg tactgaaatc
     2641 cggcaccgta agtcaaagta ccgttgtcta taccgaggcg cagcgcagct aatttattgt
     2701 attcccactc aaggccgtag tgacgatcgt tggcgtagcg cgtattactt tcgtacgata
     2761 aaagtatgtt gctcttccat tcatccaatc cttggatgta cgacatgcca aatttgaaat
     2821 tagctttgac gatatcctga gcgcgtacgt cggatgaaat tttattatac tgcacggctg
     2881 tcgtcgtggc atcctgaaga ttgaaactca ttacaaaatc accaagcgtg ggaatttcca
     2941 aaagatcatt catgccaaac atgatcatcg ttccgatatc cacgccgatg cccgacttac
     3001 gtacatcgtt gagaaccacg ccgttttctc cggagatgcc gcttgtaccg ccgcggataa
     3061 ttttgaaact tgcgccgacg ggaatttgta tagggaagtc actataggcc catccgagat
     3121 taagacgcag agtattcata cgcgcaaaag taaaaaagaa agcattatcg gcatacgaaa
     3181 agtgtccgtg ttgcagcgcc gcaaaatcaa atccgggtac agcatgttct gaagccgagc
     3241 cggatagatc cgcattttcc ggaatattgc tgatgctata gcgaatccag cctacagcga
     3301 aagaataacg gttgttaatt tgatgattgt atcctaaaac atgatactgc gcgagcccgc
     3361 caaactggct cgaataactt cccgtgacct catggcgatt aaccaatacg gaacctgccg
     3421 gattccaaaa aaatgccgtc gcgtcattgg cgatcgccga ataagcacta cccattgatg
     3481 ccgctctgac accggccggg atttccaaaa aggcattggc gtaacgtcgg ttttgtgccg
     3541 tgatgaccgt agctgcgcta atgaaaagta atattaaggt tcgaacactt tttttcatat
     3601 cgggttatca gcgaaaatac gccagtttac ctttcttctc gatagttttt ccattgagtt
     3661 tgccgcgtat acgataaaaa taaacaccat tggcaattgg gcgtccgtct tcgtccaacg
     3721 catcccaggt cacttcatga taaccaacag ctgtaatatt ttttccggac gcaatcgcgt
     3781 cggcgtctgt aattttgcga attcgacgac ccgacaccgt gtagatgctt atatccagtg
     3841 cctccaaagg ttccggagct tcgacacgga atgcaaacgt tgttgtgttg ataaagggat
     3901 tggggtaatt accatacgat tcgatgtcaa atttacgaat gacctgaaaa ttgagcgtgt
     3961 cggtcttact tgtattcccg ttgacatctt tcgccgtaaa caacacttcg tgttggccgt
     4021 catcaaaagc ctggttaaga tttaatgtaa atgtgacggt atttgaattg ggtaccgaat
     4081 ccggtaatac gatttgtgaa gcgctgatcg gtgcgttatc aacagttatc gcatagtggt
     4141 tgcgatccag acttacgcca ttgcggtcat agactatagc cgagacgcgc ggttggcgcg
     4201 gtgcaatacc accttgcgta aaaagttgcc cttcgatcgt agtttcgatc accggtgcgg
     4261 tacgatcgcg gttaatcata agcatccacg gagcacggtc ggaaatcgaa gtg
//
LOCUS       DAOQNQ010000277         4240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_9301_length_4240_cov_6.080048, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000277 DAOQNQ010000000
VERSION     DAOQNQ010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9301_length_4240_cov_6.080048"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2377
                     /locus_tag="PK754_13290"
     CDS             <1..2377
                     /locus_tag="PK754_13290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB10524.1"
                     /translation="AAGFAKDDPAVQRALKFISRCQNLPGETNDQPFAKKTTDDDKGG
                     QKARDTIILSVSPVNDAPLTSQPVADVFVNENFSKTAIAYLRSTFRDPDGDVLGYGSF
                     TAGGYTDKVTLVTSGDTLYAISKPDSFGVYVGYAKAQDAVTSVADTFRITIKGRPRVT
                     GIQSVSIDTTSFVVNASINPNGTISSLFVRYGRSVEAMNSVSDTSSHSVVETAVHTLH
                     NLIPDSTYFYRVISFSEAGFDSNSIPLSTRTLQVPSEFDQVTVSDPNPSPGTTITITA
                     KVLGSRADSVVLIYGLSKDTIGKRLRMSWNGGLLYSGTIPSASVTINGLWYRVIAYNQ
                     RGPKSFPSQGRRQNISIQVSVAAISEIKMVSQFPAGLPENDWSIVSLPVNGSLDLEKY
                     FGKQDMKNGKPTNWSAGTWNTDSNKFISAKILEGGKGYILFHRSKSKNFPFDSIANAR
                     TNAPDYFDSIVVHKGWNIIAWPYTYVDTISILAAGFVHNVWTIRDSAGSKVEWTNNSF
                     VMRPYSGFMIKLQNSYPQNTAYLGDLIRRNHKTTVVLSHPNWSIQLAAENDHYRDSKN
                     RIGISESASNGDDGMDGSKPPQFDDRVRLTVRRQEKSGLFENLSSDIRTLTDSGHVWD
                     FEIANAGTGSRFYWISTGIPDNLDAVIVDLTANKAYDIRGNNTVPLIHAEHQFKLIAG
                     NSSFVDQKMKALMETMPTTFSLKQNYPNPFNPTTRIDFDIAHTTHVRIRIYNLLGQEV
                     RTLQNSIMETGRHFVIWDGKDAHDRAVASGVYLYRLEAGAVTKVKKMLLLK"
     gene            2737..3306
                     /gene="gmk"
                     /locus_tag="PK754_13295"
     CDS             2737..3306
                     /gene="gmk"
                     /locus_tag="PK754_13295"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNB10525.1"
                     /translation="MANLSSSKGKLIVFSAPSGTGKTAIKNTLLKMDPTLVFSISATT
                     RAKREGENDGKDYYFISEEEFKDHIQNDDFVEYDHHFGNYYGTLRMAVEPDLRMGCNV
                     IMDLDVNGALNVKKSYGGTSVLIFIKPPSMDELRKRLMSRGTETEETLKIRLARVQEE
                     MDRAHLFDHVVVNDTIERAANEILDIIRR"
     gene            3308..>4240
                     /locus_tag="PK754_13300"
     CDS             3308..>4240
                     /locus_tag="PK754_13300"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNB10526.1"
                     /translation="MPADTAHNDQLRSFVLNSNETEIEKNLRSIGESIGLYGESETIR
                     TIIRDVCRVAPSDVAVLVIGESGTGKDVVAKAIHQLSARRNKPLVIVNSGAIAEGILE
                     SELFGHERGAFTGAVSERKGYFETANGGTIFLDEIGDMPLQTQVKLLRVLETGEFLKV
                     GGSVVRRCDVRVIGATNRNLEQMVRRGEFRKDLYYRLKAITITMPPLRQRRADIPVLI
                     KRFTDEYSAQTYAKFRGFTAEAMDMMVNYRWPGNVRELKNFVESLITIKRGETVTAED
                     ITKQLENFKGIDAFENDADDTMLPIPTHMTPEQAE"
BASE COUNT         1189 a          917 c         1032 g         1102 t
ORIGIN      
        1 ggcagcgggg tttgccaagg atgaccccgc ggtacagcgg gcgctcaagt tcatcagccg
       61 gtgccaaaat ctgccgggcg agaccaacga tcagccattc gcgaagaaga ccaccgatga
      121 tgacaagggg gggcaaaaag caagagacac cattattctt tcggtatctc cggtcaatga
      181 tgcgccttta acttcacaac cggtggccga tgtttttgta aacgaaaact tctcaaaaac
      241 cgcaattgcc tatctgcgca gtacattcag agatcccgat ggcgacgtgc tcggttatgg
      301 cagttttacc gctggcggat atacagacaa agtcacgctc gtgacttccg gcgatacgct
      361 ttatgcgatc agcaaacctg attcattcgg ggtgtatgtg ggttatgcca aagctcagga
      421 tgccgttact tcggttgcag atactttccg tataacgatc aaaggccgtc cgcgcgtgac
      481 gggtattcaa tccgttagta tcgacacgac ttcatttgta gtgaatgcaa gtatcaaccc
      541 caatggtacg atatcatcgc tatttgtgcg ttacgggcga tctgtggaag cgatgaatag
      601 tgtcagtgat acgtcttcac atagtgtcgt tgagacggca gtgcatacac tgcacaacct
      661 gataccggat tcgacatact tctatcgcgt catttcattc agtgaagctg gttttgattc
      721 gaactcgata ccactcagca cgcgaacgct tcaggttccg tcagaatttg atcaagtgac
      781 ggtttccgat cccaatcctt cgcccggtac gacgatcaca ataacagcta aagtattggg
      841 tagtcgcgct gactctgttg ttttgattta cgggcttagc aaagatacaa tcggtaaacg
      901 cctgagaatg tcatggaatg gtggtctttt gtattccggc acaattccct cggcaagtgt
      961 tacgatcaat ggtctttggt atcgcgtgat cgcctataat cagcgcggac ccaaaagttt
     1021 tccatcgcaa ggccgacgtc aaaatatttc aatacaagtt tctgtagcgg cgatatcaga
     1081 aatcaaaatg gttagtcagt ttccagcggg gctgccggaa aacgactgga gtatcgtttc
     1141 gcttccggta aacggatctc tggatttgga gaagtacttc ggaaaacaag atatgaaaaa
     1201 tggcaaaccg accaactgga gtgccggcac gtggaatacc gatagcaaca aatttatttc
     1261 tgcaaaaatt cttgaaggcg gcaaaggtta tattttattt catcgttcaa aatcgaagaa
     1321 tttcccattt gattctattg ccaacgcaag aactaatgcg cctgactatt tcgacagcat
     1381 tgttgttcat aaaggttgga atatcattgc ttggccttat acgtatgttg ataccatatc
     1441 catcttagcc gccgggtttg ttcacaatgt atggacgatc cgcgattccg caggatcaaa
     1501 agtcgaatgg accaataact ccttcgttat gcgtccgtat agtggcttca tgatcaagtt
     1561 gcaaaacagt tatccgcaaa atacagcgta tctaggtgac ctgattcgga gaaaccataa
     1621 aaccaccgtc gtattgtcac atcccaattg gtctatccag ctagctgccg aaaatgatca
     1681 ttatcgcgac agcaagaacc gtattggtat aagcgaaagc gcatctaacg gtgacgatgg
     1741 catggatggc agtaagccac cgcaattcga tgatcgagtc cgactgactg ttcggcgtca
     1801 agaaaagtcg ggattgtttg aaaacctatc cagtgatatt cggacgttga ccgattcagg
     1861 tcatgtgtgg gactttgaga ttgcaaatgc gggtacaggt tccagattct actggatcag
     1921 tacgggaatt cctgacaatc ttgatgctgt gattgttgat ttgactgcaa ataaggccta
     1981 tgacatccga ggcaataaca ccgttcctct tatccatgct gaacatcaat tcaaattaat
     2041 tgcaggaaat agcagttttg ttgatcaaaa aatgaaagcg ttgatggaaa caatgccgac
     2101 gacattcagc ctaaaacaaa actatcccaa tccgtttaat ccgacgacgc gaattgattt
     2161 tgatattgcg catacgactc atgtccgaat tcgcatatat aatctgttag ggcaagaagt
     2221 tcgaacacta cagaatagta ttatggaaac cggccgtcac tttgtcatct gggatgggaa
     2281 agatgcgcat gaccgagcgg tagccagtgg cgtttatcta tatcgcctcg aagcgggtgc
     2341 tgtcacgaaa gtaaaaaaga tgttattgct aaaataaaaa cgaataaaag acaaatagag
     2401 atcatttcga caagtgaagt gggggagaac tgctcgctca ttcgcaaagc agataaacgg
     2461 atagattttt ccgcgtcttt gtgagaatga atcctgttac tatttcgacc aacggatcgt
     2521 gtggaaaact tcgaacgggt tcgttatctg tattcctaat tgaattgcct ttctaattat
     2581 cctataaaaa gtaaactgcc cgaagggaaa atattatttc cctgtcttga taatcgattc
     2641 acattttacc aatttatttc ttgccttaaa cttaagtaaa atctatattt ccgcttattg
     2701 taaagtaata atctgtaaaa atgtcagtac ttaaaaatgg ctaatctttc atcatccaaa
     2761 ggcaagctga tcgtctttag tgctccatcg ggtacaggaa agaccgccat taagaatacc
     2821 ttgctcaaga tggacccgac gttggtattt tccatttcgg cgacgacacg tgccaaacgt
     2881 gaaggtgaga atgacggcaa agattattac tttatttcag aagaggaatt taaagatcac
     2941 atacagaatg atgattttgt ggaatacgat caccatttcg gaaattatta cgggacattg
     3001 cgtatggccg tcgagccgga tctccgtatg ggttgtaacg tgatcatgga tctggatgtc
     3061 aatggcgcgc tcaacgtaaa aaaatcatac ggcgggacct cggtacttat atttattaaa
     3121 ccgccttcta tggatgagct gcgtaagcgt ctcatgtccc gtggtacaga aacagaagaa
     3181 acgttgaaaa tacgcttggc gcgcgtgcaa gaggaaatgg atcgcgcgca tttatttgat
     3241 cacgttgtgg tcaatgatac catcgaacgg gctgccaacg aaattttgga tatcatccgt
     3301 cgctgatatg ccagctgata ccgcacataa cgatcaactc cgcagttttg ttctgaacag
     3361 taacgaaacg gagatcgaaa agaaccttcg ttcgatcggt gaaagcatag ggctttacgg
     3421 cgaatcagaa accatccgaa ccatcattcg ggatgtatgc cgggtcgctc cgtcggatgt
     3481 ggccgtgctg gtgatcggtg aaagcggcac aggtaaagac gtggtggcca aagccataca
     3541 tcagttgagt gcgcgtcgca ataaaccgct ggtgatcgtc aatagcggtg ccatcgcgga
     3601 gggtattttg gaaagcgaac tgtttggcca tgaacgcgga gcgtttacgg gtgcagtttc
     3661 cgagcgtaaa gggtattttg aaaccgccaa cggcggcaca atatttttgg atgaaatcgg
     3721 cgacatgccg cttcaaacgc aagtgaaact cttgcgcgtt cttgaaaccg gtgaattttt
     3781 aaaagtcggc ggttcggtcg ttcggcggtg cgatgtgcgt gtgatcggcg cgaccaatcg
     3841 caatttggaa caaatggtgc gccgtggtga atttcgcaaa gacttgtatt atcgtctcaa
     3901 agcgatcacc attacgatgc cgccgctacg tcagcggcgg gcggatatac cggtattgat
     3961 caagcggttt accgatgagt atagcgcgca gacgtatgcc aagtttcgcg gttttaccgc
     4021 ggaagcgatg gatatgatgg tcaactaccg ctggcccggc aacgtacgcg aattgaagaa
     4081 ttttgttgaa agcctgatta cgatcaagcg cggtgaaacg gtaacggcgg aagatattac
     4141 caagcagttg gaaaacttta aaggtattga tgcgtttgaa aatgatgccg acgataccat
     4201 gttgccgatt cccacacata tgacaccgga gcaagcggag
//
LOCUS       DAOQNQ010000278         4228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_9344_length_4228_cov_5.911335, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000278 DAOQNQ010000000
VERSION     DAOQNQ010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9344_length_4228_cov_5.911335"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3649)
                     /locus_tag="PK754_13305"
     CDS             complement(<1..3649)
                     /locus_tag="PK754_13305"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HNB10527.1"
                     /translation="MSYSQTISGPRMYYAQNTNPSPVIKAKLDGSSGQTVHSGLVQPW
                     SIALDIAGGKMYYTLYGGGQVRRANLDGSGDELLFSSSGARGLGLDIAAQKIYWTSWF
                     GGTVHRGNFDGTGMETIVSGLSSPTGLAVDVAGGKVYYSDNTNIRRANLDGTSQQNLY
                     SETFVWGLVLDARTQKIYWGNQNSDQIKNAPMSGGSPTVISTNSGFDQNVLAIDTIAN
                     KLYYATGLSTKQIRRINLDGSGDELLLTLPNEGRGIALDLRNHPPAALSGFFAMGQNN
                     SIVLKWRKGVEDDIIKYRIFAGPAPNPTTQIDSTTGGIGDTSKTITVANGLFQYYRVA
                     AVNAGSAQGAYSNQDVAIPAAGSGNALVFNGSSYVEIANEAAFDFTNSVTLEAWIRVN
                     SFDQVWQAIVTKGDNTYRLTRNNATNALYFAASGPGSVSGSKDVADGLWHHVAGVYDI
                     GNSELRLYVDGELDGVSGASGPIDLSDAPIWIGNNYSFPGRAWNGEIDEVRVWNTART
                     QSQIFANMHVSLRGDESGLAGLWHFDESSGTTTYDATAGGNNGTLINGPTFTASGAMP
                     LVVTSILDNGAGTLRSAINFANANPGADTIRFATNIDGATISLASALPMLTDDSTFIN
                     ADINNDSLPNIFLDGQGTTPNGFNVASTAIRATIQGFHIDGFTSNGITSDAPFTAIQY
                     NHFGLISANAQGITLTAEDNKVFRNVIAGGGTGIYGGSDATVDTTMILDNLIGTNASG
                     TVALRPGNGIYVTYRITQKGWTIKNNVINGNTNAIQLNSAFSFPLNGFTIIGNKIGTD
                     KDGVNALGGNTGILIGQNSRLHHIGDGTVPGGNLISGNSSFGIQISNTDSIWILGNYI
                     GTDVTGNASLGNNAGVSVSTSNNIWIGDGTIGGRNVISDNTTFGVGVSGGDSVRITGN
                     YIGLGVNGNTALGNQSYGVSLANDEYTIVRDNVVASNNSEGMYITGGMYRIVDNNIIG
                     LNAAGNLVRPNSAGIFMSSSKYNKIGESLGNTISGNTNDGLIFSSADSNEIYKNVIGL
                     GTAGVATFANGGTGIILQQSSANYIGNGAASGRNIISSNAGHGIRIIATAAKAENNLI
                     RNNYIGVGADGSLIRGNGQNGISLYSIPSFNTTGNYFNANVIAHNGFYGVEADGASDA
                     DSNQLDQNILFANTLGGVNYVSGSQGGVASPRIEAMFDDSTVAIQAAPGAYIQVFADA
                     GSQGEIPLITGF"
BASE COUNT         1195 a         1045 c          857 g         1131 t
ORIGIN      
        1 caaaccctgt aatcaaaggg atttcacctt gagatccggc atcggcaaaa acttgaatgt
       61 atgcacccgg ggccgcttgt attgcaactg tcgaatcgtc aaacatggct tcaatacgcg
      121 gcgatgcaac gccaccctgc gatccagaga cgtaattaac accacccaaa gtattggcaa
      181 atagaatatt ctgatccaat tgattactat ccgcatcact ggctccgtca gcttcgacgc
      241 cataaaaacc gttgtgcgcg atgacattcg cgttaaaata attaccggtg gtgttaaaag
      301 aaggaatcga ataaagactg ataccatttt gtccgttacc tcggatcagg gatccgtcag
      361 ctccgactcc gatgtaatta ttgcgaatca gattattttc tgccttggcg gccgtagcaa
      421 tgatacgtat accatggccc gcgttcgaag aaataatatt acgaccagag gccgctccgt
      481 ttcctatgta attagcgctg gactgctgga gtattatccc ggtacctcca ttggcaaacg
      541 tagccactcc tgccgttccg agaccaatga cattcttata tatttcattg gaatccgcag
      601 atgaaaaaat caagccgtcg ttggtatttc ccgaaatggt attacccagc gactcgccta
      661 ttttattata cttcgaagaa gacataaaaa tgccggcgct gttgggacgc acaagatttc
      721 cagccgcatt caaaccgatg atattgttgt ccacaatacg gtacatacca ccggtaatat
      781 acatgccctc gctgttgttt gaagccacca cgttgtctcg tacgatggta tattcgtcgt
      841 tcgccaacga tacaccgtag ctttgattcc caagcgccgt gttcccattg acgccgaggc
      901 cgatatagtt tccggtaata cgaacactgt cgccgccgct aactccaaca ccgaacgtag
      961 tattatcgga aatcacatta cgcccgccaa tcgtgccatc gcctatccat atattattgg
     1021 atgtggacac agatacgccc gcgttgtttc ccaacgacgc attaccggtc acatcagttc
     1081 ctatataatt acctaaaatc catattgaat ccgtattgct tatctgaata ccaaaactcg
     1141 aattaccgga aatcagattt ccaccgggca ctgtaccatc gccgatatga tgtagtcttg
     1201 aattttgacc gataaggata cccgtatttc cgcccagtgc gttaactccg tctttgtcgg
     1261 taccgatttt attcccaatg atcgtaaaac catttagcgg gaaagaaaat gcgctattaa
     1321 gttgtatcgc attcgtatta ccattgatga cattgttttt gatcgtccag cctttttgag
     1381 taatacgata agtgacataa ataccgttgc ctggacgcaa tgcaacagta cccgatgcat
     1441 tcgtaccgat cagattatct aaaatcatcg tagtatcgac ggtagcatcg gaaccaccgt
     1501 aaatacctgt tccaccgccc gcgataacat tacggaacac tttgttatct tccgccgtca
     1561 acgtgatccc ttgcgcatta gcgctgataa gcccgaaatg gttatactga atcgccgtaa
     1621 acggcgcgtc gctcgtaatg ccattggatg taaatccgtc aatatgaaac ccttgtatcg
     1681 tggcacgaat cgccgtcgag gctacgttaa aaccgttagg cgtagtacct tgtccgtcca
     1741 aaaatatatt aggtaaactg tcgttattga tgtccgcatt gataaacgta gagtcatctg
     1801 tcaacattgg aagtgccgat gcaagtgaga tcgtagctcc atcaatatta gttgcaaaac
     1861 gaatcgtatc agcgccggga ttagcattgg caaaattaat agccgatcgt aatgtacccg
     1921 caccattgtc aagaatcgaa gtaactacca aaggcatagc gcccgacgcg gtaaatgtag
     1981 ggccattgat cagcgtaccg ttgttacccc cagccgtagc atcgtatgtc gtcgtaccac
     2041 ttgattcatc aaagtgccaa agcccggcaa gacctgactc atcacctcgg agcgatacat
     2101 gcatattggc aaagatttgc gattgggtac gcgccgtatt ccatacacgg acttcgtcta
     2161 tttcgccatt ccacgcgcga cccgggaaac tatagttatt tccaatccaa atcggcgcat
     2221 ccgataaatc aatcggaccc gatgcgcctg aaacaccatc caattcacca tccacataaa
     2281 ggcgcagttc ggaatttcct atatcataca cccctgctac gtgatgccat aatccgtcgg
     2341 caacatcttt ggaaccgctg acgctaccag gacccgacgc agcaaaatac aatgcgttcg
     2401 tcgcattatt acgcgtcagg cgatatgtat tatcaccttt ggtaacaatc gcttgccaca
     2461 cttgatcaaa actattaaca cgaatccagg cttccaacgt tacggagttc gtaaaatcaa
     2521 aagccgcttc gttggcgatt tccacatacg agctaccatt gaaaacgagc gcgtttccgg
     2581 aaccagccgc tggaattgca acatcttggt tggagtaagc accttgggca cttccggcat
     2641 ttacagccgc tacgcggtaa tactgaaaca aaccgttagc aactgttatc gttttcgatg
     2701 tgtcgccgat acctcccgta gtcgaatcaa tttgggttgt cggattgggc gcgggacctg
     2761 caaaaatacg atatttgata atatcatctt ccacaccctt cctccatttc aaaacaatac
     2821 tattattttg acccatggca aaaaaaccgg acaacgcggc cggcgggtga tttcgaagat
     2881 ccaaagctat tccacgacct tcgttgggca atgtcaagag tagctcatct ccgcttccgt
     2941 ccaaattaat tcggcggatt tgtttagttg agaggcctgt agcgtagtac aacttattgg
     3001 caatggtatc aatagctaaa acattttgat caaatccgga attagttgaa attaccgtag
     3061 gggatccacc gctcatgggc gcgtttttga tttgatcgga attttgattc ccccaataaa
     3121 ttttctgtgt tctagcatct aaaacaagcc cccaaacaaa tgtttcagaa tacaaatttt
     3181 gttgagaagt accgtctaaa ttagcccgac gaatattcgt attgtcggaa tagtatactt
     3241 taccacctgc aacatccaca gccaaacctg tcggagaaga taaaccgctg acaattgttt
     3301 ccatcccggt tccgtcaaaa ttacctctat ggacagtacc gccaaaccaa gacgtccagt
     3361 agattttctg agccgcaata tctagcccta aaccacgcgc cccacttgat gaaaacaaca
     3421 attcatcgcc actcccatcg agattagccc ggcgaacttg tccgccgccg tacagtgtgt
     3481 agtacatttt accaccggca atatccagcg ctatactcca aggctgtaca aggccggaat
     3541 gtaccgtttg accgctactt ccatcaagtt tagctttgat taccggcgaa ggatttgtat
     3601 tctgggcgta atacattctt ggaccactga tcgtctgcga atatgacatc ggaactgtca
     3661 ctaatccaag aagccaaaag cagagtatcc aagaaactgc aaatgtagca atttgttgag
     3721 ttaccctttt cacagaaatg aatgtttttt cgttggataa aaaaggaaaa ttatctgagg
     3781 cttgtaaaac tcttcccatg agtttctccg aattagtgaa tttattttta attattttta
     3841 atcagtattt ttgcgtacat agcccgatca gatcaaacat gtataaaaaa ttaagcgcta
     3901 tattaataag tagttgagtg gtcttactgc atattcatgt agtaagttat cagtatgcac
     3961 aattaaaaca cttcatcgct gtgacgagcg tcacaaaaaa tagatttttt aactaatcgt
     4021 cagttttttg acacataata ccataaacgc ggtatgctgt caatcatttt tatgaaatac
     4081 cccaaacgcg acaaccccgt ctcatcaacg gggttgtgca gtagaaaggt atttatgtaa
     4141 agcgatcagt agagtttggc caaggcctcc agtttattga gcaagagggt cgctataccg
     4201 ggctgattgg acgccgccgt aaaggatg
//
LOCUS       DAOQNQ010000279         4226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_9353_length_4226_cov_6.019899, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000279 DAOQNQ010000000
VERSION     DAOQNQ010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9353_length_4226_cov_6.019899"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..1051)
                     /gene="panB"
                     /locus_tag="PK754_13310"
     CDS             complement(233..1051)
                     /gene="panB"
                     /locus_tag="PK754_13310"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012928885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNB10528.1"
                     /translation="MSTHHTITKVTTEQLRRMKQSGEKISALTAYDAIMASLLDESGM
                     DMILVGDSASTVFAGNDTTLPMTMEQMLYHVRVVTKSVKRALVVADMPFMSYHVSVEE
                     AIRNAGRFMQEGHAEAIKLEGGIEFLPTIQRLVEIGIPVMGHLGLTPQSIHKFGTYRT
                     RGKQPDEAKKIISDAKALEKAGVFALVLEKVPVTLAKKISASITIPTIGIGAGPHCDG
                     QILVTHDMLGLYEKFHPRFVRRYAELGKAMRKAFASYHQDVKSNRFPTDKESYS"
     gene            complement(1058..>1663)
                     /locus_tag="PK754_13315"
     CDS             complement(1058..>1663)
                     /locus_tag="PK754_13315"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNB10529.1"
                     /translation="PVGEMSDKEEVREVASRLHLRTAKKKESQEICFITDNNYNRFLK
                     EKISGLSEKLAHGMIKTHDGQVVGEHDGYAFYTIGQRRGVGVAMNEPVYVTEISAKDN
                     TIYVGRKDDLLATGLTATLVNLCRHEAITEAMPITAKIRYNDVGHEGVLTLNEIGETQ
                     VIFNQPQRAITPGQSVVFYDGDEVIGGGIIDRVLKETLQPV"
     assembly_gap    1664..1763
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1764..2071)
                     /locus_tag="PK754_13320"
     CDS             complement(<1764..2071)
                     /locus_tag="PK754_13320"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HNB10530.1"
                     /translation="MEIQIKQKKRVVVAMSGGVDSTASAALLKEQGYEVIGVTMKLWN
                     YDDVGGGEINRESGCCSIDTMHDARVVCEQLGVPHYVWDLSDEFGRAVIDNFVNEYLE
                     G"
     gene            complement(2077..2709)
                     /locus_tag="PK754_13325"
     CDS             complement(2077..2709)
                     /locus_tag="PK754_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10531.1"
                     /translation="MKHIFFGLCFFALNLSLVAQEEFDETAFESGDAKLGNGWGMNVQ
                     VSTVGFVIGGVYNYKVAQHTWLTSSLDLFWVNGENEQQVYDYNTGQTISINSETILML
                     PLVFQMKRRLFPETMSSNLRPFIVGGGGVVYGWFIDSDVSKSKLPPPQENTQYTYTLI
                     GGIGADIGKPGSTGYGLDVKYQWLRYSNYLGARKKFDNIQVGFHVNFRSN"
     gene            complement(2730..3185)
                     /locus_tag="PK754_13330"
     CDS             complement(2730..3185)
                     /locus_tag="PK754_13330"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB10532.1"
                     /translation="MDLSGTYIPPDQQRSPDFQLTIPSMLDKLNAVEEITERVADAFH
                     LRDDDRDNLAIAVTELTNNAIIHGNKFDPKKNVILSFYNDDGVLKVYIRDFGKGFDPG
                     AVDNPLDPENLLKESGRGIFILRSIMDDVQFMFAGDGTVVKIVKRFLPK"
     gene            complement(3201..3836)
                     /gene="upp"
                     /locus_tag="PK754_13335"
     CDS             complement(3201..3836)
                     /gene="upp"
                     /locus_tag="PK754_13335"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNB10533.1"
                     /translation="MISFANLHVIRHPLVQQKLTYLRDKNTSHREFRKLLSEITAFMI
                     FEITQDLKTKDIQVETPLETTTAQVLADHVVLVPVLRAGLGMLDGALDLIPNAKVGHI
                     GLYRNEQTLEPVEYYAKFPPTLPEATAIMLDPMLATGGSSSAAVALLKRKRARHIKCL
                     SLVAAPEGVERMLKDHPDVMIYTAGLDRQLNEHGYIVPGLGDAGDRLYGTK"
     gene            complement(3843..>4226)
                     /locus_tag="PK754_13340"
     CDS             complement(3843..>4226)
                     /locus_tag="PK754_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10534.1"
                     /translation="QYVYDTKRGNATRTDIEYLDMLVKYSRKVMPDVAVIVPIHAQLH
                     WLDEGLLPDDIGFVFEGFDGINYDAPFPKLTELERQFAKNHITLVPRLPVFPDFIDRI
                     DASESLRSVLADWQSKRMYVTYAKA"
BASE COUNT         1065 a         1037 c          898 g         1126 t
ORIGIN      
        1 tatgcaccgg tttatacgac gcgttggcat caggaagtcg gcgcttcagt caacgggtta
       61 ttcggtttat tccgcataga tgccgcacaa cgtttggata aaaagaattt ttaccttggg
      121 atgagcatgg cgcgcttgtt ttaaacaaac aacatataac aaaaattaac agagtgaaaa
      181 ttaaccgttt tcactctttt ttttgaaaaa attaacggat ttaaaaaaac tactacgaat
      241 agctttcttt gtcggtggga aatcggttgc tctttacatc ctgatgatag gaagcaaagg
      301 ctttgcgcat agctttacca agctcggcat agcgccgcac aaaccgcggg tgaaattttt
      361 catataaccc aagcatatca tgcgtgacca atatctggcc gtcacaatgc ggcccggcgc
      421 cgatacctat ggtcggtata gtaatagatg ccgatatttt tttggctaaa gtaactggaa
      481 ctttttcgag aacaagcgca aacacgccgg ctttttccag agctttggcg tcactgatga
      541 tttttttagc ttcatccggt tgtttgccgc gtgtacggta cgtgccaaat ttatgaattg
      601 actgcggcgt aagcccgagg tgacccatca cgggtatgcc gatttcgacc aatcgttgta
      661 tcgttggtaa aaactcgatc ccgccttcca atttgatggc ctccgcgtga ccttcctgca
      721 taaaacggcc cgcattacga atagcttctt cgacgctcac atggtaactc ataaacggca
      781 tatccgctac gaccaatgcg cgtttgaccg acttggtaac gacacgcaca tgatacagca
      841 tctgctccat cgtcatgggc aatgtcgtat cattgccggc aaataccgta ctggccgagt
      901 cgccgactaa gatcatatcc attcccgatt catccaaaag agaagccatg atcgcgtcgt
      961 aagccgtcaa tgcgctgatc ttttcaccgc tttgtttcat gcgacgcaac tgttctgtcg
     1021 tgactttggt tatggtatga tgagtggaca tgctatctta aaccggttgc agcgtttctt
     1081 tgagtacacg atctatgatc ccgccgccga tcacttcatc accgtcataa aatacgaccg
     1141 attggcccgg cgtaatggcg cgttggggtt gattaaaaat tacctgggtt tcaccgattt
     1201 cgttgagtgt gagaacgcct tcgtggccga cgtcgttata tcggattttg gccgtaatag
     1261 gcatggcttc cgtgatcgct tcatgccggc aaagattgac cagtgtcgcg gtaagccccg
     1321 ttgccaaaag atcatcttta cgaccgacgt aaatggtgtt atctttggcg ctgatctccg
     1381 tcacatatac aggttcattc atcgcgacac ctacaccacg acgctgaccg atcgtataaa
     1441 aagcatatcc gtcgtgttca ccgacgactt gcccgtcatg ggttttgatc attccgtgcg
     1501 caagcttttc ggaaaggccg cttattttct ctttgagaaa acggttgtaa ttattatccg
     1561 tgataaaaca aatttcctgg ctttcttttt tcttcgccgt tcgcaaatgc aggcgcgacg
     1621 cgacttcgcg aacctcttct ttgtccgaca tttcaccgac gggnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnccttcaa ggtattcatt gacaaagtta tcaatcacgg
     1801 cgcgaccaaa ttcgtcgctc aaatcccaca cataatgcgg tacgcctaac tgttcgcata
     1861 ccacacgcgc atcgtgcatg gtgtcaatgg agcaacaccc gctttcccga ttgatctcgc
     1921 cgccgcctac gtcatcgtaa ttccacaact tcatcgttac gccgatgact tcgtatccct
     1981 gctccttgag caaggcggca gaagccgtac tgtccacacc gccgctcatc gcgacgacaa
     2041 cgcgtttttt ttgtttgatc tgtatttcca tactgtttag ttggagcgaa agttaacgtg
     2101 aaacccgacc tggatattat caaatttttt gcgggcgccg aggtaattcg aataccgcag
     2161 ccattggtat ttgacatcaa gtccgtatcc tgtcgaaccc ggtttgccaa tgtctgcgcc
     2221 aataccgccg atcaaggtat atgtgtattg ggtattctcc tgcggtgggg gtaatttact
     2281 tttgctgaca tcactgtcaa taaaccagcc atacaccaca ccgccgccgc cgacaataaa
     2341 cggcctcaaa ttgctggaca ttgtctccgg gaatagtctg cgcttcatct gaaaaacaag
     2401 cggcaacatc agaatagttt cactattaat actgatggtt tgcccggtat tataatcata
     2461 aacctgctgc tcgttttcac cattgaccca aaaaagatcc agtgaagatg tcaaccatgt
     2521 atgttgtgcg actttatagt tatacacacc gccgataaca aagccgaccg tggatacctg
     2581 gacattcata ccccagccgt tcccgagttt agcatcaccg gattcaaacg cagtttcatc
     2641 aaattcctcc tgcgcgacaa gagaaaggtt cagtgcaaaa aagcacaacc cgaaaaatat
     2701 atgtttcata cgaacccctt tcgttttcat tatttcggta gaaagcgttt tacgattttg
     2761 accactgtac catcgccggc aaacataaac tgtacgtcgt ccataataga acgcaaaata
     2821 aagattcctc ttccgctttc tttcaataaa ttttcaggat ccagtggatt atccaccgca
     2881 cccggatcaa aacccttgcc aaaatcgcga atatacactt tgagaacgcc gtcatcgttg
     2941 taaaaactga gtatgacatt ctttttagga tcaaatttat tgccgtgaat gatcgcgttg
     3001 ttcgttagct cggtcacggc tatggcgaga ttatcacggt catcgtcgcg aaggtgaaac
     3061 gcatcggcca cacgctccgt gatctcttcg accgcattca gcttatccag catactcggt
     3121 attgtaagct gaaaatcagg tgatcgctgc tgatccggtg gtatgtatgt tcctgataaa
     3181 tccaagatac gtcgttaaac ctatttagtt ccgtaaagac gatcaccggc atcgcccaaa
     3241 ccgggtacga tatagccgtg ttcattgagt tgacgatcga gcccggctgt ataaatcatc
     3301 acgtcgggat gatctttgag catgcgctct acaccttccg gtgctgcgac aagagacaaa
     3361 catttgatat gccgtgctct tttacgtttg agcaaagcca ccgccgccga agagctccct
     3421 ccggtggcca gcatcggatc gagcatgatc gccgtagctt ccggcagagt cggcggaaac
     3481 ttggcatagt attccacagg ctccagcgtt tgttcattgc gataaaggcc gatgtgaccg
     3541 actttagcat tcggaataag atcaagcgcg ccatccagca ttcccaaacc tgcacgcaat
     3601 accggcacaa gcacgacgtg atcggccaat acctgcgccg tcgtagtttc aagcggcgtt
     3661 tcgacctgga tatctttggt tttcagatct tgtgtgattt caaaaatcat aaatgccgtg
     3721 atttcactga gcaatttgcg aaattcacga tgggatgtat ttttatctcg caggtaagtc
     3781 agtttttgct gaaccagcgg atggcgtatg acatgaagat tggcaaatga tatcatagga
     3841 gatcaagcct ttgcataggt tacatacata cgtttggatt gccagtcggc taagacgctg
     3901 cgtaaacttt ccgatgcatc aatgcgatca ataaaatccg gaaacaccgg caaacgggga
     3961 acaagcgtaa tatgattttt ggcaaactgc cgttccagtt ccgttaattt aggaaatggc
     4021 gcatcataat tgattccgtc aaaaccttca aacacaaatc cgatatcatc cggcaaaagg
     4081 ccttcatcaa gccaatgtaa ttgagcatgt ataggcacga tcacggcaac atccggcatg
     4141 acctttcggc tgtattttac caacatatcc agatattcta tgtcggttcg tgtcgcattc
     4201 ccgcgtttcg tatcatagac atattg
//
LOCUS       DAOQNQ010000280         4201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_9451_length_4201_cov_5.526532, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000280 DAOQNQ010000000
VERSION     DAOQNQ010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9451_length_4201_cov_5.526532"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PK754_13345"
     CDS             <1..556
                     /locus_tag="PK754_13345"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HNB10535.1"
                     /translation="DRPALLLVDLQKGFDTISYWGNERNNPGAEENCRWLLELWRSKD
                     LPVVHVQHCSANPNSLLADTHPGNAFKDAVQPLPWETVIKKNVNSAFIGTTLQKHLTV
                     NSISKLVIVGLTTDHCISTTTRMAGNYGYEVFLISDATATFDKTGMDGKKYSAELIHQ
                     TALASLHGEFATVFPMKTLLQKFS"
     gene            613..2637
                     /locus_tag="PK754_13350"
     CDS             613..2637
                     /locus_tag="PK754_13350"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HNB10536.1"
                     /translation="MKTPGLILLLLLVNLSACKTDKPHPYRSLAQEADTTRRPGDDFF
                     SYANGKWIASTVMPEGKERWNARHEITELTKKQVAKIFEEAQTAPHGTNARKVADFRA
                     AYMNESAIQAAGITPIKPLLDEIDPVTDKTALVKLLGRRLRADADWLTYRSPGLLGLS
                     VEVGYFGEKTYVPFLMQGGLGLEERSYYLSEDAANKKIRATYQNYISQQFKQAGFSHP
                     ERRAQSVLGIEILLAKTHASDEFSNNDRNGSHRWSRNDFERRAPGMDWSVFFEEAGLK
                     HQESFVVWQPDAITGLASLIAAQPVEVWKDYLSFQAIHAYADVLPSAFAEAALTLRSS
                     IQDAPSPARSQRALDATQKFLGEAVGWLYVERYFPASQKSRVQAIATNVIASLRERVK
                     TVSWMSDESKTQAMVKLQTLYFGLGYPNVWQDYSDLNIDPANPVKNLQNIADRNYRRA
                     LARLGKPPDTTEWATTPHTAGALLLFQYNACNFAAALLQAPKFDSTLSDAANYGAIGA
                     IVGHESSHFIDPLGAEWNAERELKHWWTPNDMKKFQSAAKPLVEQFSGYRPFDDLAVN
                     GELTRSENIADLAGLAAAFDAYRKTLGDKIQNQDYVRQQDREFFIGFAQSWRSKTTEK
                     GLRVQIATDHHAPEQYRIATVRNLDAWYDAFDVKPGDKLYLTPESRVRIW"
     gene            2729..4129
                     /locus_tag="PK754_13355"
     CDS             2729..4129
                     /locus_tag="PK754_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HNB10537.1"
                     /translation="MLFILGVLAVMGTNDFDAYFTGQTMRFDYYHSGTAKEEHISPDE
                     YRIEGVWPGSKTVLLDETGLGKYLFEVSDVASGKLLYTRGFASIYGEWETTGEAAKGV
                     WRSFHESQRFPEPKNAVKLTLKKRGADNRFASFYETTIDPKSRFVNRAPAVKNGEVWS
                     VFENGDPSQKVDLLILSDGYTAGEKNKFHADVERLVKVMFETEPFKSRKKDFNVRAMD
                     VASAQSGISNPRKDIWRKSALGLSFNSFDSDRYVLTYENKSLRETAANAPYDALILLA
                     NDRKYGGGGIFNLWATVTADAEPASYVFVHEFGHSFAGLGDEYYTSPVSYENFTAPST
                     EPSEPNITALFDPKNLKWKHLVSKDTPLPTPWNKTAYDTYEIEMQKKRAKLIEEKASE
                     ETMEALFREVKTKTTPMLNTDKYFGKVGAFEGAGYEAKGLYRPELDCIMFTRNNVPFC
                     KVCHEAIERIIDLYAK"
BASE COUNT         1156 a         1026 c         1002 g         1017 t
ORIGIN      
        1 agatcggcca gcgcttttat tagttgatct tcaaaaaggt tttgatacga tttcttattg
       61 gggcaatgaa agaaacaatc ccggggccga agaaaattgc cgttggctgc tggaattatg
      121 gcgcagcaaa gatctgcccg tcgtgcatgt acaacactgc tcggccaacc cgaattctct
      181 tctggccgac acgcatccgg gaaacgcctt taaagacgcc gtccagcctt tgccgtggga
      241 aaccgtaatc aaaaaaaatg tgaacagcgc ctttatcggt accacacttc aaaaacatct
      301 cactgtcaat tctatttcca aactcgtcat cgtcggactc accaccgatc actgtatatc
      361 cacgacgacg cggatggccg gaaactatgg gtacgaggta tttctgatct ctgatgccac
      421 ggcgacattt gacaaaaccg gcatggacgg aaaaaaatat tcggctgaac tgattcatca
      481 gacggcgctg gccagtcttc atggtgaatt tgcaaccgtg tttccgatga aaactttatt
      541 acaaaaattt tcttaaaaaa tattgaacgc ggccgatagc atcggacccc ataaactaac
      601 cggattttta tcatgaaaac acctgggctg attttacttt tgttacttgt gaatttaagc
      661 gcttgcaaaa ccgataaacc acacccctac agatcgctgg cgcaagaggc ggataccacg
      721 cgtcgtccgg gcgatgattt tttttcgtat gccaacggaa aatggatcgc atccaccgtt
      781 atgccggagg gcaaagagcg ctggaatgcc cgtcatgaaa taacagagct taccaaaaag
      841 caggtcgcta aaatttttga agaagcacag acggcgcctc acggaacaaa tgcacgtaaa
      901 gtggccgatt ttcgtgcagc ctacatgaat gaatcagcca tccaggcggc cggtatcaca
      961 ccgatcaaac ccctgctgga tgaaattgat cccgtaaccg ataaaacggc gttggtcaaa
     1021 ttactcggtc gcagactccg tgccgatgcc gattggctta cgtatcgttc cccgggatta
     1081 ttgggtttgt ctgtggaagt aggatatttc ggcgaaaaaa cctacgtgcc ttttttaatg
     1141 cagggcggcc ttgggctgga agaacgttca tattacctca gcgaggacgc ggccaataaa
     1201 aaaatcagag caacctatca gaactatata tctcagcaat tcaaacaagc cggatttagc
     1261 catcccgaac ggcgtgcaca gtcggtactt ggcatcgaaa tcttattagc aaaaacccat
     1321 gcgagcgatg aattttccaa caatgaccgc aatggttcgc atcgctggtc acgcaacgac
     1381 tttgaacgac gggctccggg tatggattgg tccgtttttt ttgaagaggc cggtctgaag
     1441 catcaggaat cgttcgtagt ttggcagccg gacgcgatca ccggattggc ttcgcttatc
     1501 gccgcacagc ccgtggaagt gtggaaggat tatctgagtt ttcaagcgat ccatgcgtac
     1561 gccgatgtat taccgtcggc tttcgcggaa gcggcgctga ctttgcgctc gtcgatacaa
     1621 gacgcacctt cccctgcccg ttcacaacgt gcgctcgatg cgacacagaa atttttgggt
     1681 gaagccgtcg gctggttata tgtagaacgt tattttcccg cttcccaaaa atcccgcgta
     1741 caagcgatcg cgacgaacgt catcgcttcg ctccgcgaac gggtcaaaac cgtgtcgtgg
     1801 atgtcggacg agagcaaaac acaagcgatg gtaaaacttc agacattgta ctttggtctc
     1861 ggttatccca atgtatggca agattattcg gatctgaata tagaccctgc caatcccgta
     1921 aaaaatttac aaaacatcgc cgatcgcaac tatcgccgcg ctttggcacg gcttggaaaa
     1981 ccgccggata ccaccgaatg ggcgacgaca ccccataccg cgggcgctct gcttttattc
     2041 caatacaacg cctgcaattt tgccgccgcc ttactacaag cacccaagtt tgattccaca
     2101 ttatcggatg cggccaacta tggcgctatc ggcgctattg tcggtcacga atcaagccac
     2161 tttattgatc ctttgggagc cgaatggaat gctgaaagag agctaaagca ttggtggaca
     2221 cccaacgata tgaaaaagtt tcaatcggcc gccaaaccgc tggtagagca gttttccggt
     2281 tatcgcccgt ttgacgacct cgccgttaac ggtgagctta cccgatcgga aaatatcgcc
     2341 gaccttgcgg gacttgccgc cgcttttgat gcctatcgaa aaaccttagg ggataaaatt
     2401 caaaatcagg actacgtacg ccaacaggat cgtgagtttt ttatcggttt tgcacaaagc
     2461 tggcgcagca aaaccaccga aaaaggcctg cgcgtacaaa tcgcgacgga tcatcacgca
     2521 ccggaacagt accgcatcgc taccgtacgc aatctcgacg cgtggtatga cgcatttgat
     2581 gtcaaacccg gtgataaact gtacctaaca ccggaatcgc gcgtacgcat ctggtaatgg
     2641 aaaattatag aatatgacgg catgtttttg atatattggt gtgcattatt catcgcgttt
     2701 atcacgctaa ttatctcagg agaatatcat gctatttata ttgggagtac ttgccgtcat
     2761 gggcactaat gattttgatg cttattttac cggccagacg atgcggtttg actattatca
     2821 cagcggtacg gccaaagaag aacatatcag tcccgacgaa taccgcatcg aaggcgtgtg
     2881 gccgggcagt aaaacggtgt tattggatga aaccggtctc ggcaaatatt tgtttgaagt
     2941 gagtgatgtt gcatccggaa aattgcttta tacgcgcgga tttgcgagca tctacggcga
     3001 atgggaaaca acgggcgaag cggccaaagg cgtgtggcgc tcatttcacg aatcgcagcg
     3061 ttttcccgaa cccaaaaatg cggtcaaatt aacactcaaa aaacgtggcg ctgataatcg
     3121 ttttgcgtcg ttttacgaaa caaccatcga tcccaaaagc cgctttgtca atcgtgcgcc
     3181 tgccgttaag aacggcgaag tgtggagcgt ttttgaaaac ggcgaccctt cgcaaaaggt
     3241 tgatctgctg attctcagcg acggttacac ggccggcgaa aagaataaat ttcacgccga
     3301 cgtcgaacgt ttagtcaaag tgatgtttga aaccgaaccc ttcaaatccc gcaaaaaaga
     3361 ttttaatgtt cgtgcgatgg atgtagcttc cgcccaatcc ggtatcagta acccgcgcaa
     3421 agatatttgg cgcaaatcgg cgctcggttt gagcttcaat tcgtttgact ccgatcgtta
     3481 tgtactgacg tatgaaaata aaagtcttcg tgaaacggcc gccaatgcgc cctacgatgc
     3541 attgatcctt ttggccaatg atcgcaaata cggcggcggc ggtattttta atctgtgggc
     3601 gacggtcacg gccgatgcgg aaccggcttc gtatgttttt gtacacgaat tcggtcattc
     3661 ttttgcgggt ctcggcgatg aatattatac ctcacccgtt tcgtatgaaa actttaccgc
     3721 gccgagtacc gaaccttccg agccgaatat taccgctttg tttgatccga aaaatctcaa
     3781 gtggaaacat cttgtatcta aagacacccc tttgccgaca ccgtggaata aaaccgcgta
     3841 cgatacgtat gaaatcgaga tgcaaaaaaa acgcgctaaa ctaatcgaag aaaaagcgtc
     3901 cgaagaaacg atggaagccc tctttagaga agtaaaaacc aaaacgacgc cgatgcttaa
     3961 tacggacaaa tatttcggaa aagtgggcgc ttttgaaggc gcaggttacg aagccaaagg
     4021 tttatatcgc ccggaattag attgcatcat gttcacacgc aataacgtcc ccttttgtaa
     4081 agtatgccac gaagcgatcg agcgaattat agacctgtat gccaaataag aaagagttta
     4141 tacaaacctt agtcattcat acgtcgccgg aaaatatctg gcgcgtatgg atggctgtag
     4201 a
//
LOCUS       DAOQNQ010000281         4197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_9464_length_4197_cov_5.200628, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000281 DAOQNQ010000000
VERSION     DAOQNQ010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9464_length_4197_cov_5.200628"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..>4197
                     /locus_tag="PK754_13360"
     CDS             26..>4197
                     /locus_tag="PK754_13360"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB10538.1"
                     /translation="MASKTPKRVSSKKKSPQKTLRKSHSTRQRDALLHLYRLRKGFDA
                     LVRAAVRISADLQSIDRVSVWLFTPERSAIQCVALYSREKKKYFQGDVLLASKYKPYF
                     KALNSGRSLLANNAQKDPRTTAFTKDYLLPNGIGAMMDVPIWQDGRVIGVLCHEHVGG
                     SRRWTRREEEFANAVADCITGSKHAEERRQAEIRYHALLEASDDMVFIEDYYRNVLYI
                     NPAVEKHTGYTAADYDFNVHQHPLIYPEDLPNVNRFIADFIQSEAMFSKTLENRFVDR
                     WGRLRWHSSVLSKIEYLQKPAIQFVVSDITGQKFALDALHRSEERFTMLVRQLRDLIT
                     IVSADGTIRYESPSIFPMLGYTQDELLGRNAFDQIHPEDLEVLQKTFESLLRAPGESA
                     TAMYRFLHKNGTWRHCESVGVNLLLDASVEGILITTRDVTERVEAEQLLKEREERYRK
                     LVESFHDILFITDFKRNMLYINPAMERLTGYTLNDFRFPPDDNPFVHPEDFKRVNAFI
                     DEFVQSAKTHSSIIENRFFDRWGTMHWYSSVISKIEYDDQPCLQFICRDISDVRRSEE
                     ALTQSDQKYRAFIEQSTEGIYRLELDEPVPIDWPPEEQVANIFARGYIAEANTAQALM
                     YGFNSVDELIGLRLKTLLLPEDPHNVAYMVDFVKSGYRFANQESHEIDKYGVQKIFLN
                     SAIGIVEDGKLIRVWGTQKDITELKKSEIALKQSEERYRAFVTNSSEGICLFEFETPI
                     AVNLPAEDQIASFFETAFISECNSTYALSEGYRAPDQLTGIKVWRNEGAEQAAQRDWV
                     AYLIRNGYSASDIESEKISKDGSKRFFLNSYTGVVEQGFWKRVWAVRKDVTERRLVEE
                     DLRNNEANLAAILENSHDSIWSIDAAYRIVTINSTFRDAYYAAFGIRLEVGMNVMDSI
                     PDDLGALWKARYERVIVRGEVFMESDHYDLGGESRDYEISFFPIRTDDGIIRGASIFS
                     RDVTERVRSREAVERREKYYRALIENALDLISIISADGTILYESPSVEKITGFTPNDL
                     IGKNVLDFAHPEDRPLIREAFQTGIHQGQKIVNVHFRFLHKDHSWRTLEVTAHNLLHD
                     AVVGGIVVNSRDITERKKSEEVLRQSEEKFRLLAENLPGVVYMCENDADFTFIYTSEA
                     ILDITGYPKEDFYDRRISLSMMYHPDDVDMINHIVHTAVRDQHSFSLTYRIRHRNGSW
                     RWLSEVGSGVYADGKLLYIEGFISDITELRTAESILRQRDLQYKTLVEQALNPVYVLQ
                     NEKIVVVNPAWEKLFGYTAHDVSQPDFSIYKIIAPENKPMIEERMERRRQGLPVSDLY
                     ELKVITKDGRIRDLEVRVAEILWNDTPAIQGVYYDITDRKKTERALEAEKERLSVTLR
                     SIGDGVITTDVQG"
BASE COUNT         1148 a          879 c         1051 g         1119 t
ORIGIN      
        1 tggtgttaac atagtttttt gagttatggc cagtaaaacc ccaaagcgag tttcatctaa
       61 aaagaagagt ccccaaaaga ctcttcgaaa atcacatagc acgcgacagc gcgatgctct
      121 tttgcatctg tatcgtctgc gtaaaggttt tgacgctttg gtgcgcgcgg cggtacgtat
      181 atccgccgat ttgcaatcta ttgatcgcgt cagtgtttgg ttattcacac cggaacgatc
      241 ggctatccag tgtgtcgcac tttacagccg ggagaaaaaa aagtatttcc aaggggacgt
      301 acttttagcg tctaaatata aaccttactt taaagcattg aattccggcc gttccctcct
      361 ggcaaacaat gctcaaaaag atccccgcac tactgctttt acgaaggatt atcttttgcc
      421 caatgggatc ggtgcgatga tggatgtgcc gatctggcag gacggacgtg ttatcggtgt
      481 tttgtgccat gagcatgtcg gaggatcacg ccgatggaca cgacgcgaag aagagtttgc
      541 caacgctgta gccgattgta tcaccggctc aaaacatgcg gaagaaaggc gccaagccga
      601 aatacgatat cacgctctac tcgaagcgtc ggatgacatg gtatttatcg aggactacta
      661 tcgcaatgta ttgtacatca accccgctgt cgaaaaacac acgggataca cggccgcaga
      721 ttatgatttt aatgtacatc aacatccgtt gatttatccg gaggatttac ccaacgtcaa
      781 tcgtttcata gcggatttta tacaaagcga agccatgttt tccaaaactt tggaaaaccg
      841 ttttgttgat cgttggggga gattacgctg gcattcgtcc gtacttagta aaatcgaata
      901 ccttcaaaaa ccggccattc agtttgttgt cagcgacatc accgggcaaa aatttgcctt
      961 agacgcattg catcgcagtg aagaacgatt tacgatgctg gtgcgccaac tgcgcgatct
     1021 gatcacgata gtttcagcgg acggtacgat ccggtacgaa agcccttcca tatttccgat
     1081 gctcggttat actcaggacg agttgcttgg gcgcaatgca ttcgatcaga ttcatccgga
     1141 agaccttgaa gtattgcaaa aaacttttga atccttgctt cgtgcgccgg gcgaatcggc
     1201 tacggctatg taccgttttt tgcacaaaaa cggaacgtgg cgtcactgtg aaagcgtcgg
     1261 tgtcaatctg cttttggacg catccgttga gggaattctc attacgacac gcgatgttac
     1321 cgaacgtgtg gaagctgaac agttgctcaa agaacgggaa gagcgttaca gaaaactagt
     1381 ggagtcgttc catgatattc ttttcatcac ggatttcaaa cgcaatatgc tgtatatcaa
     1441 tccggcgatg gaacgtctta ccggttatac actcaacgac tttcgttttc cgccagacga
     1501 taatcccttt gttcacccgg aagattttaa aagagttaat gcgtttattg acgagtttgt
     1561 gcaaagcgcg aaaactcatt caagcattat agagaatcgt ttttttgatc gttggggtac
     1621 gatgcattgg tattcatcgg tgatttccaa aatagaatac gacgatcaac cgtgtctgca
     1681 gtttatttgc cgtgatattt ccgatgtacg tcgttctgaa gaagcgttga cccaaagtga
     1741 tcaaaaatac cgcgctttca tcgagcaaag tacggagggt atttaccgcc ttgagttaga
     1801 tgagccggtt ccgattgatt ggccgccgga agaacaagta gctaatatat ttgcccgggg
     1861 ttatatcgcc gaggccaata ccgcgcaagc gctgatgtat ggttttaatt cggttgatga
     1921 attgatcggc ttacgcctga aaaccttact tttaccggaa gacccgcata acgtggcata
     1981 tatggtggac ttcgtaaaat cgggttatcg ttttgccaat caagaatcgc atgaaataga
     2041 caagtacggc gttcagaaaa tatttttgaa cagcgccatt ggtattgtcg aagacggtaa
     2101 acttatacgc gtatggggga ctcagaaaga tattacggaa ttaaaaaaat cagagattgc
     2161 gctcaaacaa agcgaagaac ggtatcgtgc gtttgtgacc aatagctctg aaggaatttg
     2221 tttatttgaa tttgaaacac cgattgccgt gaacctaccc gccgaagatc agatcgcatc
     2281 tttttttgaa acggcattta tcagcgaatg caatagtacg tatgcgctca gtgaaggtta
     2341 tcgtgcaccg gatcagctca cggggatcaa agtttggcgc aatgaaggtg cggagcaagc
     2401 ggcacagcgc gattgggtag cctacttaat tcgtaacggt tattcggcga gcgatattga
     2461 gtcggaaaaa atatccaaag atggttccaa acgatttttc ctcaacagtt ataccggtgt
     2521 cgtggagcaa ggtttctgga aacgcgtgtg ggccgtacgc aaagatgtaa ccgaacgacg
     2581 gctggtcgaa gaagatttga gaaataacga agccaatctg gcggcgattc ttgaaaattc
     2641 gcatgattcc atttggtcca ttgatgcagc ctatcggatt gtcacgatca attcgacatt
     2701 ccgcgatgca tattatgcgg catttgggat tcgtctggaa gtcgggatga atgtgatgga
     2761 cagcataccc gacgatctcg gggccttatg gaaggcaagg tatgaacgcg tcatcgttcg
     2821 cggtgaggta tttatggaat cggatcacta tgatctgggc ggcgagtcgc gtgattatga
     2881 aatttcattt tttccgatcc gaacggacga cgggattatt cgcggcgctt ccattttttc
     2941 gcgcgatgtg accgaacgtg tgcgaagccg agaagccgtg gaacgtcgtg aaaaatacta
     3001 tcgcgcgttg attgagaatg cgttggatct tatcagtatt atttcggcgg atggtacgat
     3061 cttgtacgaa agtccttccg tagaaaaaat aaccgggttt acgcctaacg accttatcgg
     3121 taagaacgtg ttagattttg cccatccgga agaccgtcct ttaattcgcg aagccttcca
     3181 aaccgggatt catcaagggc aaaaaatagt caacgtccat tttcgatttt tacacaaaga
     3241 tcattcatgg cgtacattgg aagtgacggc ccataaccta ttgcatgatg cggttgtcgg
     3301 cggcatcgtg gttaattcgc gtgacattac ggaacgaaaa aaatcggaag aagtgttgag
     3361 gcaaagcgaa gagaaatttc gattacttgc cgaaaatctt cccggtgtcg tgtatatgtg
     3421 tgaaaacgat gctgatttta cgttcatcta taccagcgaa gcgattttgg acattacggg
     3481 gtatcccaag gaggattttt atgacaggcg gattagtctg agtatgatgt accatcctga
     3541 tgatgtggat atgatcaatc atattgttca taccgccgtg cgggatcagc attcgtttag
     3601 tcttacctat cgtatccgac accgcaacgg aagctggcgg tggttatcgg aagtcggttc
     3661 cggtgtgtat gcggatggta aacttttata catcgaggga tttatttctg atatcaccga
     3721 attgcggacg gcagaaagca tacttcgtca acgcgatctt cagtataaaa cactagtcga
     3781 acaagctttg aacccggtgt atgttttgca aaacgaaaaa atcgtagttg tcaatccggc
     3841 gtgggaaaag ctcttcggtt atacggcgca tgacgtatca caaccggatt tttcgatata
     3901 caagattatc gctcccgaga ataagcctat gatcgaagag cgaatggaac ggcgacgtca
     3961 aggattaccc gtatcggatt tgtatgaact aaaggtaatc accaaagacg gccgtattcg
     4021 tgatttggaa gtgcgggtag ctgaaatttt atggaatgat acaccggcta ttcagggtgt
     4081 atattatgat ataacggatc gcaaaaaaac cgagcgcgct ctcgaagccg aaaaagagcg
     4141 gttatctgtg acactgcgtt cgatcggtga cggcgtgatc acgacggatg tgcaagg
//
LOCUS       DAOQNQ010000282         4168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_9573_length_4168_cov_5.327984, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000282 DAOQNQ010000000
VERSION     DAOQNQ010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9573_length_4168_cov_5.327984"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PK754_13365"
     CDS             <1..279
                     /locus_tag="PK754_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB10539.1"
                     /translation="KYTEKGRVDVRAHVENKKLIVEVEDTGNGIKQEDMGRIFERFYR
                     VDRGRSREQGGSGLGLAIVKHVMEIHKGTVRLESEVGKGSRFILTFPV"
     gene            285..806
                     /locus_tag="PK754_13370"
     CDS             285..806
                     /locus_tag="PK754_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10540.1"
                     /translation="MKSIQLLSQLELTCIKPDDHQKRFQIMDGADVVGELSYAKSAGT
                     LARAESGDAIWTFKRCGLWKPYISIRKEGTHTNLARIILGWHNVLMSEPPLRRVHFRN
                     SSFWHNEWSWVTDEDETIMKFKPAHDHHMRGKIMIHEKFRQDGWLMMMALLGWYMLVS
                     LEDEAGDTMVVMS"
     gene            complement(808..1467)
                     /gene="ftsE"
                     /locus_tag="PK754_13375"
     CDS             complement(808..1467)
                     /gene="ftsE"
                     /locus_tag="PK754_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HNB10541.1"
                     /translation="MIELSNVTVRYRNRTSLENASLRIAPGEFVYLLGPSGAGKSTLL
                     KLLYMDIIPEEGQVRIGEFESSNVKKKEIPYLRRQLGIVFQDFRLLRDRSVYDNIAFT
                     LLVTETPKSEIPKKVMRALAQVGLSTKKNSMPYELSGGEQQRVVIARALVNEPMVILA
                     DEPTGNLDPDTSMEIMQLLTKINAEGTAIVMATHDYNIVKALPKRIIRVEGGKITDAP
                     N"
     gene            complement(1471..2196)
                     /locus_tag="PK754_13380"
     CDS             complement(1471..2196)
                     /locus_tag="PK754_13380"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNB10542.1"
                     /translation="MRLMIVTIAAVLTVVSCKTTQPAAKSDADFAKALETSEKNKSEK
                     LDFLADEKLPNDMITPPDTASPMTPAPDSITYKTYEPTIQARFRVQVFAGSPSNAQKN
                     YTSLSADTTWKKAVYVLDDKTDGKWRVWVGDFMTREEADAAKQKLIQNGYPDAWIHEM
                     KDMTTPKISGKELFWLQIGSFSTEAAAQKAKTEAESKQTVRSKISHTDGMYKVWIGGF
                     EDRAKADELKKTFSKAFIVKGAE"
     gene            complement(2218..2529)
                     /locus_tag="PK754_13385"
     CDS             complement(2218..2529)
                     /locus_tag="PK754_13385"
                     /inference="COORDINATES: protein motif:HMM:NF017922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HNB10543.1"
                     /translation="MLNHETATDVTIDRGKKLVTIQWADGKLSELPYAYLRAACPCAN
                     CDTTKHGGEANPLYPEQFADITMSDVQEVGRYALRFTWSDGHDTGIYSYSYLREKSNL
                     F"
     gene            complement(2523..3287)
                     /locus_tag="PK754_13390"
     CDS             complement(2523..3287)
                     /locus_tag="PK754_13390"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB10544.1"
                     /translation="MTPELMPGAEPFFYTSETPAHNAVLLVHGFTSSCHSMRELGKRI
                     YENGMDARGILLPGHGTTPDDLATRYWSEWYEAVNIAFLEMKKNYNKVFIAGQSMGGC
                     LALLLAARNHAVDGAVTIASGLKIYNTGIGLVPLVKHFIKHKPKLYGPDVKDERVKQT
                     EVHYTRMPYASIHELLKLLKSLRAEIGNVTCPLLTIHGREDHTFDFRNQDMIYRRVAS
                     TQKKKVVLENTYHLATLDFDKTIVQNETINFLKSLC"
     gene            complement(3289..3681)
                     /locus_tag="PK754_13395"
     CDS             complement(3289..3681)
                     /locus_tag="PK754_13395"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNB10545.1"
                     /translation="MEFSKEEMMEIMAVFKAESAENLQILNDKMKEIAANPSNPAAIE
                     QLHRTSHSMKGAARMLGLTPIEEIGRALEDGFKVVKDGKPLLTPDMIENVNVAVLGMA
                     QLIDKLANEGTTDGFDISGIMQRLAAFK"
     gene            3814..>4168
                     /locus_tag="PK754_13400"
     CDS             3814..>4168
                     /locus_tag="PK754_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HNB10546.1"
                     /translation="MEIVLATSNPKKVKEMNAIMSGMQVRFRRLNEFSDVPEIIEDGL
                     TFEANALKKAHVIFEHTGIMALADDSGIEVDALGGKPGVFSERYAGPAQDDRANLEKL
                     LRDTEHVPDEKRTARF"
BASE COUNT         1013 a          954 c         1044 g         1157 t
ORIGIN      
        1 aaatatacgg aaaaagggcg cgtggatgta cgcgcacatg tcgaaaacaa aaaactgatc
       61 gttgaagtcg aagataccgg caatggtatc aaacaagaag acatggggcg tattttcgaa
      121 cgattttacc gtgttgatcg tggtcgttcg cgggaacaag gcggaagcgg tcttgggctt
      181 gcaattgtca aacatgttat ggaaattcat aagggaactg tacgtctcga aagtgaagtc
      241 gggaaaggtt cccgctttat tctcactttt cctgtgtgag tcgtatgaaa tcaatacaat
      301 tactctcgca gcttgagctt acctgcatca aacccgatga ccatcaaaaa cgatttcaga
      361 tcatggatgg agccgacgtg gtcggtgagc tgagctatgc caaatcggcg ggcacacttg
      421 cccgcgccga gtccggcgat gcgatttgga cgttcaaacg atgcggtctt tggaagccgt
      481 acatctcaat ccgtaaagag ggcacgcaca ccaatctggc gcgcatcatt ctcggatggc
      541 acaatgtgct gatgtcggag ccgccgctcc ggcgcgtaca ttttcgcaat tcgagctttt
      601 ggcacaacga atggtcgtgg gtgaccgatg aagatgaaac gatcatgaaa ttcaaaccgg
      661 cgcatgatca tcatatgcgc ggaaaaatta tgattcatga aaaatttcgt caggacggat
      721 ggctcatgat gatggcattg ctgggctggt atatgctggt ttcgcttgaa gatgaagccg
      781 gcgatacgat ggtcgtgatg tcctgattca gttgggtgcg tcggtgattt tgccgccttc
      841 gacacggata atgcgtttgg gaagcgcctt cacgatgtta taatcgtggg tggccataac
      901 gatggctgtg ccttccgcgt ttattttggt cagcagctgc atgatctcca tcgaagtatc
      961 cggatcaaga ttaccggtcg gttcgtctgc gaggatcacc atcggttcgt tgacaagtgc
     1021 gcgtgcgatg acgacgcgct gttgttcgcc gccggataat tcgtagggca tgctgttctt
     1081 tttggtggaa agccctactt gggccagtgc acgcatcact tttttcggaa tttcgctctt
     1141 gggcgtctcc gtgacgagca acgtaaacgc tatattatcg taaaccgaac gatcccgtaa
     1201 taaccgaaaa tcctgaaata caatacccag ctgtcggcgc aggtaaggga tttccttttt
     1261 tttgacattc gatgattcaa attcaccgat gcgcacctga ccttcttccg gtatgatatc
     1321 catatataat aatttaagca gtgtgctttt gccggcaccg ctaggtccca acaaatatac
     1381 gaactcaccc ggagcgatgc gtagcgaagc gttttccaag ctggtgcgat tgcgataacg
     1441 tacggttacg tttgaaagtt cgatcatcgg ttactccgcg ccttttacga taaacgcttt
     1501 ggaaaacgtt tttttcaatt catcggcttt cgcgcggtcc tcaaacccac cgatccatac
     1561 cttgtacata ccgtcggtat gggaaatttt agagcgtact gtttgtttgg attcggcttc
     1621 cgtttttgct ttttgcgctg ccgcttcagt agaaaaagat ccgatttgaa gccagaaaag
     1681 ttctttaccg gaaatcttcg gcgtagtcat atctttcatt tcatgaatcc acgcatcggg
     1741 ataaccgttt tgtattagtt tctgtttggc ggcatcggct tcttcccgtg tcataaaatc
     1801 gccgacccag acgcgccact tgccgtcggt tttgtcatct aaaacataga cggctttttt
     1861 ccatgtggta tcggcggaga gcgatgtata attcttctgc gcgttggagg gactgcctgc
     1921 aaacacctgt acgcggaaac gcgcctggat cgtaggctcg taagttttat aagtgatcga
     1981 atcgggcgca ggcgtcatcg ggcttgccgt atccggggga gtgatcatat cattgggcag
     2041 tttttcatcg gccaaaaaat ctaatttttc tgatttattt ttttccgacg tctcaagcgc
     2101 tttggcaaag tcggcatcgg atttagccgc aggctgcgtg gttttacaag aaactaccgt
     2161 aagcacagcc gcaatggtca caatcatcaa acgcatacat tttctccgat aggtgtatta
     2221 aaacaaatta cttttttcgc gcaaatagct gtatgaatat atgcccgtgt catggccgtc
     2281 cgaccaggta aaacgcagag catagcgccc gacttcctgc acgtccgaca tcgtaatatc
     2341 cgcaaattgc tccggataca gtgggttggc ttctccgccg tgtttggtcg tgtcacaatt
     2401 ggcgcagggg catgctgcgc gcagataggc gtacggtaac tcgctgagtt ttccgtcggc
     2461 ccattggatc gtgactaatt ttttaccgcg gtctatcgtg acgtccgtag cggtttcgtg
     2521 atttagcata atgatttcaa aaaatttatg gtttcgtttt gtactattgt tttatcaaaa
     2581 tccaacgtgg ctaaatgata ggtgttttcg agaacaactt tcttcttttg ggtcgacgcg
     2641 acgcgacgat aaatcatgtc ctggttgcga aaatcaaacg tatgatcttc acgtccgtgt
     2701 atcgtaagca acgggcaggt tacattgccg atctcggccc gcaacgattt caacagtttg
     2761 agcaactcat gaatactggc gtacggcatg cgtgtgtaat gcacttccgt ttgtttgacg
     2821 cgttcatctt tgacatccgg cccgtacaat tttggtttgt gttttataaa atgtttgact
     2881 aacggcacca acccaatgcc ggtattatag attttcaatc ccgatgcgat ggtaactgca
     2941 ccgtctacag catggtttcg cgccgcgagc aataaagcga ggcatccgcc catagattgt
     3001 ccggcgataa aaactttgtt gtaatttttt ttcatttcta aaaaagcaat attaaccgct
     3061 tcataccatt ccgaccaata acgtgtagcg aggtcgtcgg gtgtagtacc gtgtcccgga
     3121 agcagtatgc cgcgtgcatc catgccgttt tcataaattc tctttccgag ttcgcgcatc
     3181 gagtggcaac tggaagtgaa accgtggact aatagtacgg cgttatgtgc cggggtttcc
     3241 gaagtgtaaa agaacggttc ggcgccgggc atgagctccg gggtcatatc acttaaacgc
     3301 cgcgaggcgc tgcataatac cgctgatatc aaagccgtcg gtagtgcctt cattggccaa
     3361 tttatcaatg agttgggcca taccaaggac ggcaacattc acgttttcga tcatatcggg
     3421 cgtcaggagc ggtttcccgt ctttgacgac tttgaatccg tcttccagcg cacgcccgat
     3481 ctcttcgata ggggtcagac cgagcatacg tgctgcgcct ttcatgctgt gggaagtgcg
     3541 gtgaagctgc tcaatcgctg caggattgga agggttcgca gcaatttctt tcatcttatc
     3601 attaagtatc tgaagatttt cggcgctctc ggctttgaaa acggccataa tctccatcat
     3661 ttcttctttg gaaaattcca tacacacctc ggaatgtttc aggtcggtga atgtaggcga
     3721 tttctctaaa aacttcaatt tgttttccgc acgaataatt cctttagcct ttgctctttt
     3781 tttatgacat tggtgaatac aacggagcac acgatggaaa ttgttttagc aacaagtaat
     3841 cccaaaaaag taaaagaaat gaatgctatt atgagcggga tgcaagtccg gttcagacgg
     3901 ctgaatgaat tttccgatgt accggaaatc attgaggacg gattgacgtt tgaagccaat
     3961 gcattaaaaa aagcgcatgt gatatttgaa catacgggga taatggcgct cgcggatgac
     4021 tcgggtatcg aagtggatgc gctgggcgga aaacccggtg ttttttcgga acggtatgcc
     4081 ggcccggcac aagacgaccg tgctaacctg gaaaagttat tacgggatac agagcatgtg
     4141 ccggatgaaa agcgtaccgc acgctttc
//
LOCUS       DAOQNQ010000283         4138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_9688_length_4138_cov_6.148175, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000283 DAOQNQ010000000
VERSION     DAOQNQ010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9688_length_4138_cov_6.148175"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..1810
                     /locus_tag="PK754_13405"
     CDS             47..1810
                     /locus_tag="PK754_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HNB10547.1"
                     /translation="MNIAVAMLVSLGALWLAYRFYGSRVAQWLGVDVSRPTPAQRVND
                     GVDFVPTKPVVLLGHHFASIAAAGPIVGPTMALLYGYMPTWLWIILGVTFIGAVHDFS
                     ALFVSVREDGRSIAEIAKRTLGKTGFLFFVAFAILLCILVCAAFLQLTAVSLTSLYPL
                     KEFGLPEDQNILKTVTDNGVLKGVIGGIASTSVIVMTLVAPLIGYLLYKKESGILTVS
                     LLAFIFCLVSVWVGFVYPVTLDPKIWMMVICAYCFIAGWIPVWLVLQPRDFTNVQLLY
                     FGIVVMVAGIIGSGLAGVVIDAPAFNFSAESFQALGYMWPFLFVTIACGSVSGAHALI
                     ATGTTSKQIGSEKHAPMIGYGAMLLEALLGICVTLIILGGLGYTDYHDIVWPHDEFGN
                     PKAGNAPLAFAVGVGHTLDKGLGISVVYGTVFGIILLEGFLVTTIDTIVRLTRYLFEE
                     LWLTLMENPHPIFRNKFFNTLVPVIGMIALGFTNAYTAIWPIFGSANQLLAALTLTAI
                     TAWLIQKSRPFWFTAIPAIFMCLTTITSLSLLFKRYLDARNWALTIADAILLLLTVGV
                     VIMTFRYFYNLRKELSSKKSV"
     gene            1819..2532
                     /locus_tag="PK754_13410"
     CDS             1819..2532
                     /locus_tag="PK754_13410"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB10548.1"
                     /translation="MKNYLLLYIILVFAGVVSAQTGLARRQADQLYDRGDNFHAMEAY
                     KNLLNDNPNDPELNYNAGNALYRLEQYDEAVKFYQRALENTSDADLKNKIAYNMANIQ
                     YKQKKLKESIEGYKNVLRKNPDDADARKNLEYALKQMQMQQPPPQKNQDKNQSKQDKK
                     EQDQKNQDDQNQPQDENKEEQQNDEQQNKKKSDPNKSGLTKKEAEKLLDALKNQEKDY
                     QKKKIKDKAKSDSETEKDW"
     gene            2564..>4138
                     /locus_tag="PK754_13415"
     CDS             2564..>4138
                     /locus_tag="PK754_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10549.1"
                     /translation="MFYTSIQLTFAWSVFFLIVIVGLSVGLSAWLYRETIPPVSQVRR
                     RILISLRSAAFAIAFFILFEPILDLQYEEKEKPVVAVLIDASESMSIRDAQGDRQQQR
                     RGVLDHRMWADHADAWDIRYFTFSDRLYPSDHKPSDTLALTGHATDMRTALEQTQSLF
                     IGKHLAAYVMITDGQYNIGGDPGGFAQVSDVPVFTLGIGDPAESRDVAIVQISHNETA
                     YLGATIPVTVTLSSYGYQGKRIDVEILHNGKRVQNKIVVLPEDGALTQVTMDVIPDEI
                     GDQKYTVRASPLLGELSDRNNQRAFVIRVLKNKTSVTLISGAPGPDHRFIYSVLIENP
                     NLDVRAYVEQKNGTLTELEQPPGRTSPTEADVYIFNDYPTNTSDPSTLQRFLQDISDN
                     NRSAAIFFGNHIEVGRLQSIQSRLPIKAKPDASLQESSVFIGMSGSASQSTIVAVSDD
                     PGETMRQWADLPPVWISRYAVTASEESDVLARVDMSRTTNTLRLRNDIPLLITKRLRQ
                     QKTLAFTGYGFWKADFI"
BASE COUNT         1066 a          918 c         1013 g         1141 t
ORIGIN      
        1 tgttaaaacg gattttcaaa ccttggacat ttcgtcggag taacgcatga atattgccgt
       61 agcaatgctg gtttcactcg gtgcattatg gttggcttac cgtttttacg gtagccgtgt
      121 tgcacagtgg ttgggtgtag atgtatctcg gccgacgccc gcgcagcggg taaatgacgg
      181 tgttgatttt gttccgacca aacccgtcgt attactcggg catcattttg catccattgc
      241 tgcggccggg ccgatcgtag gtccgacgat ggctttgttg tacggctata tgccgacgtg
      301 gttgtggatt attttaggtg tgacgtttat cggcgcggtg catgattttt cagcgttgtt
      361 cgtcagcgtt cgtgaagacg gacgatccat cgcggagatt gccaaacgta ctcttggaaa
      421 aaccgggttt ttgttttttg tagcgtttgc cattttactc tgcattctcg tctgcgcggc
      481 ttttttacaa ttgaccgccg tatccctcac gtcgctgtac cctttaaaag aatttggctt
      541 gccggaagat cagaatattt taaaaacggt tacggataat ggcgttttaa aaggtgtcat
      601 cggcggcatc gcatcaactt cggtgattgt tatgacctta gttgcgccat tgatcggtta
      661 tcttctctat aaaaaagaat ccggtatttt aacggtcagc ttactggctt ttattttttg
      721 ccttgtttcg gtgtgggtag gatttgttta tcccgtgaca cttgatccta aaatttggat
      781 gatggtgatc tgcgcttatt gttttatcgc aggttggatt ccggtttggc ttgttttgca
      841 accacgggat tttaccaatg tacagttgtt atacttcggt attgtcgtaa tggttgccgg
      901 tattatcgga agcggcttgg ccggtgtagt gatcgatgcg ccggcgttca atttttccgc
      961 agaatcgttt caggcgctcg gttatatgtg gccgtttctt tttgtgacaa ttgcctgcgg
     1021 ttcggtatcc ggagcgcatg ccctgatcgc caccggtacg acatccaaac aaataggcag
     1081 cgaaaaacat gcgccgatga tcggttacgg tgcgatgctt ctggaagcgt tgctgggcat
     1141 ttgcgtgacg ctcatcatat tgggcgggct tggttatacg gattatcacg acatcgtctg
     1201 gccgcatgac gaatttggaa atcctaaagc cggtaatgcg ccattagcgt ttgccgtcgg
     1261 cgtagggcat actttagaca aagggcttgg tatttcggtg gtgtacggta cggtgtttgg
     1321 tattattctg ctcgagggat ttctggtcac aacgatagat accatcgtgc gtctgacacg
     1381 ttatcttttt gaagaattgt ggctcacgtt gatggaaaat ccccatccga tatttcgtaa
     1441 taaatttttt aatacgttgg tacccgtgat cggcatgatc gcgcttggtt ttaccaatgc
     1501 atacaccgcg atttggccga tttttggttc agccaatcaa ctgctggcgg cgcttacact
     1561 gacggctatt accgcatggc ttatacagaa atcgcgccca ttttggttta cagcaatccc
     1621 ggcgattttt atgtgcctta cgaccattac atcgctttcg cttttattta aacgctattt
     1681 ggatgcacgc aactgggcgc tgacgatcgc cgatgcgata ctgttgctgc tcacggtggg
     1741 tgtcgtaata atgactttcc gttattttta taatttacgt aaggaactca gttccaaaaa
     1801 atccgtatga gacgggatat gaaaaactat ttactgcttt atatcattct tgtgttcgcc
     1861 ggcgttgtat cagcacaaac aggccttgcc cggcgtcagg cggatcagct ctacgatcgg
     1921 ggcgataatt ttcacgctat ggaggcgtac aaaaatttgc tcaatgataa tcccaatgat
     1981 ccggagttga attacaatgc cggcaacgcg ctgtaccgtt tggagcagta tgacgaagcg
     2041 gtcaaatttt atcagcgcgc attggaaaac acgagcgatg ccgatctcaa aaataagatc
     2101 gcatataaca tggcgaatat tcaatacaag cagaaaaagc ttaaagaaag tatcgagggc
     2161 tacaaaaatg ttctgcgtaa aaatcctgat gacgccgacg cacgaaaaaa cttagaatac
     2221 gcgttgaaac aaatgcagat gcagcaaccg ccgcctcaaa aaaatcagga taagaatcaa
     2281 tccaagcagg ataaaaaaga acaggatcaa aaaaatcagg acgatcaaaa tcaaccgcag
     2341 gatgaaaaca aagaagaaca gcaaaacgac gaacagcaaa ataagaagaa gtccgatccg
     2401 aataaatccg gtttgaccaa aaaagaggct gaaaaattac tggatgcgct caaaaatcag
     2461 gaaaaagatt atcaaaaaaa gaaaataaaa gataaagcca agtcggattc cgagacggaa
     2521 aaagactggt aattttgtat atattaatga acaacgaggc cttttgtttt atacttcgat
     2581 acagttaact tttgcctgga gtgttttttt tctcatcgtg attgtcggac tttccgtcgg
     2641 attatccgct tggctctacc gtgaaaccat tcctcccgtg tctcaagttc gtcgccggat
     2701 acttataagt ttgcgcagcg cggcttttgc cattgctttt tttattttat ttgaacccat
     2761 acttgatttg cagtacgaag aaaaagaaaa accggtagtt gcggtattga ttgatgcgtc
     2821 cgaaagcatg agcattcgcg atgcgcaagg tgatcgccaa cagcaacggc gcggcgtact
     2881 cgatcatcgt atgtgggcgg atcatgccga tgcgtgggat atcagatatt ttacctttag
     2941 tgatcgttta tatccgtccg atcataagcc atccgataca ctagccttga caggccatgc
     3001 taccgatatg cgtaccgcgc tagagcaaac ccaatcgctg tttataggca aacacctggc
     3061 cgcgtatgta atgattacgg acggtcaata caatatcggc ggcgatcctg gcggatttgc
     3121 tcaagtatcc gatgtaccgg tttttacgct tggtatcggc gatccagcgg aatctcgcga
     3181 cgtagccatt gttcaaattt cacacaatga aaccgcttat ctgggagcga cgatcccggt
     3241 aaccgtgacg ctttcatcgt acggctatca gggaaaacgt attgatgtcg aaattttgca
     3301 caacggaaag cgcgtgcaaa acaaaatcgt tgtgttaccg gaagacggag cgctgacgca
     3361 ggtgacgatg gatgtcatac ccgatgaaat tggtgatcag aaatatacgg tgcgcgcaag
     3421 tcctttgctc ggcgaattat ccgatcggaa caatcaacgc gccttcgtca tacgtgttct
     3481 caaaaacaag acatccgtta cactgatctc cggagcgccc ggtccggatc atcgttttat
     3541 ttacagcgtc cttatcgaaa atcccaattt ggacgtacgt gcgtacgtcg aacaaaaaaa
     3601 cgggacgcta accgagttgg aacaaccgcc cgggcgaacg tcaccgacgg aagcggatgt
     3661 ttatattttt aacgactatc cgacgaatac gtctgatcct tcgacgttac aacgctttct
     3721 gcaggacata tccgataaca atcgcagtgc tgccatattc ttcggaaacc atatcgaagt
     3781 cgggcgatta caatccattc agtcgcgact tcccatcaaa gctaaaccgg acgcgagttt
     3841 gcaggaatcc tccgtcttta tcggtatgag cggatcggcc agccagagca cgatcgtggc
     3901 cgtttccgac gatccgggag agaccatgcg acagtgggcc gatttgccac cggtttggat
     3961 cagccgttat gctgtgacgg catctgaaga gtcggatgtt cttgcgcggg tagatatgtc
     4021 gcgaaccacg aataccttgc gtttacgaaa cgatattccg ctgctgataa ccaaacgctt
     4081 gcggcagcaa aaaacattag cctttacggg ctatggattc tggaaagcgg attttatt
//
LOCUS       DAOQNQ010000284         4133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_9710_length_4133_cov_5.649338, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000284 DAOQNQ010000000
VERSION     DAOQNQ010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9710_length_4133_cov_5.649338"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="PK754_13420"
     CDS             complement(<1..913)
                     /locus_tag="PK754_13420"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNB10550.1"
                     /translation="MSKILVTGCAGFIGSHVTDALLKAGHEVVGLDNFDHFYDPAIKE
                     KNLTNAIQSPGFRLLRGDILDVDLMGRIFSESKFDLVIHLAARAGVRPSIQDPKIYQK
                     VNVEGTMNILEAMRAHNVKKLLLASSSSVYGNNEKVPYHETDNVDHAISPYAATKKAC
                     EVLAYTYHHLFGIDAFCLRFFTVYGPRQRPEMAIHLFTRLIDEGKPITLFGDGQTFRD
                     YTYVDDIVHGIMQCIDGLRGYEILNLGESQTISLIDLVNTIEKALGKKAQIRWMPNQP
                     GDVQKTYADISKARHLLGYKPSVGIEEG"
     gene            complement(910..3171)
                     /locus_tag="PK754_13425"
     CDS             complement(910..3171)
                     /locus_tag="PK754_13425"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNB10551.1"
                     /translation="MSDLNDDKQGIDARAILQTVLMHKWIVLTVFIVFSGIVSYYSLS
                     KPTLYRSSALLFFDNGLSNLAFANVPQQQKIALGSQDFGYYEVIITTDLFNQRFRKEL
                     ERVVLSERNDAETVLALKNVSSTQVTLKPYKEVEQFIEISAVNYDSFLVKKMIEVTTD
                     VLKSRVTELDREGLQRSIEFIDEQVEVSRNNIEKTEVELQALQKKTDVNIKQSEGPLA
                     KVLIMKEKLAEFETQIQIRSSNINALNSQLDSIQRRLTGSSSYAANESTEEKQIKLQI
                     EQLTSQKAVLYEKLGSDAVENPEVQRIDRQIADLRSQYYTLLSSVKTGSDNTVSGDMS
                     QVWKTIWDKKNTEEIELFLLKGQARLFSGLITNFERKNPNLLQDAIDISRLLRLKQVY
                     EERLSSLIKQKESLSIQYYGSTGSLKIIDPAKEPVAIYNKVFTNIFVGTVLGLIIGIA
                     IAVGLEFMDNRIKSIAAISSVTSIAVVGRIPPINVGDKDNAVEGLRKKINKIKTKDEE
                     EDKNEIRRKAMISQFNPRSFVSERYRSLRTNIQFANIDSPIRSLLVASAGPGEGKTTT
                     AVNLAISFADMGHRVCIVDTDLRKAKHHMLFDIQEQPGLTDIILGQTDLDTALQKTSI
                     NNLHSLTVGRDAKDHSEIFSSLRMNFMMNELEKKFDIVVYDTAPVMLLTDSVILASKV
                     DAVLMVVKHAVTPKSQLQNAIHNLKGVRANIIGIVMNDYADERKNYYKYAYDGYYLSK
                     SQRISTSEEGVKA"
     gene            complement(3198..>4133)
                     /locus_tag="PK754_13430"
     CDS             complement(3198..>4133)
                     /locus_tag="PK754_13430"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNB10552.1"
                     /translation="VMSMSSSAGELHAQTKDSLKADALSQIRADRKIRPGDILDVVVY
                     GHDELPKQIKVEADGTIKYPFMKDIKVDGMSVEQLTNILSARLSQYIGGRAEVIVAYA
                     KDEMIEVIVLGSVAQPGSHKIPRNHTIQGAIATAGGATVRSDLDRTKLIRINPETSLR
                     EEIEVPMVSIIVETGDIRKLPALRDGDVIFVPSIYGAVYANVLGAVGKPGNYELFPGA
                     NLVDVIFLAGGPKDDASIRTVKLIRRIGVAQTEEIVDVEAVLKAKGGSVPLVQPGDIV
                     FVPAKKFTLKTIFQVLSYAATILTLIFLSKQAGLL"
BASE COUNT         1143 a         1000 c          788 g         1202 t
ORIGIN      
        1 ccccttcttc tataccgacc gaaggtttat aacctaagag gtgccgcgct ttacttatat
       61 ccgcataagt tttctgtaca tcccccggct gattgggcat ccaccgaatt tgagcttttt
      121 ttcctaatgc tttttctatc gtattgacca ggtctatgag ggaaatcgtt tgcgattcgc
      181 cgagatttaa tatttcatat ccgcgaagac cgtcaatgca ttgcattatc ccatgaacaa
      241 tatcatccac atacgtataa tcgcgaaacg tctgtccatc accaaataaa gtgatcggtt
      301 tgccttcatc tatcaaacgg gtaaacaaat gaatcgccat ctcaggcctt tggcgagggc
      361 catacaccgt aaaaaatcgt aagcaaaacg catcgatccc aaataaatga tgataggtat
      421 aagccaaaac ttcacatgcc tttttggtcg cggcatatgg tgatatggca tgatctacat
      481 tgtctgtctc atggtaagga actttttcat tattaccata cacggatgaa gaggacgcga
      541 gtaggagttt ctttacattg tgggctcgca tcgcttccaa aatattcatc gtaccctcaa
      601 cattgacctt ctgataaatt ttgggatctt ggatggaggg acgcaccccg gcacgtgccg
      661 ccaaatgaat taccaaatca aacttcgatt cggaaaaaat acgacccatc aaatcaacgt
      721 ctagaatatc accacgtaac aaccgaaaac cgggactttg aatagcgtta gtaaggtttt
      781 tttctttaat cgcaggatca taaaaatgat caaaattatc caaaccgacg acttcgtggc
      841 ccgctttcaa aagtgcatcc gtaacatgcg aaccgataaa acccgcacat ccggtcacta
      901 atattttact catgctttta caccttcttc gctcgtgctg atacgttgtg acttggaaag
      961 ataatacccg tcgtatgcat atttataata attcttccgc tcatctgcgt aatcattcat
     1021 cacgataccg atgatgttgg cacgaacacc ttttaaattg tgaatggcat tttggagttg
     1081 cgacttcggc gttacggcat gtttgaccac cataagcacc gcgtcaactt tagaagccag
     1141 aataaccgaa tccgtcaaca acattacagg cgcggtatcg tatacgacga tatcaaactt
     1201 cttctccaat tcattcatca taaaattcat acgcaagctg gagaaaattt ccgaatgatc
     1261 tttagcgtct ctaccgacgg taagcgaatg aaggttgtta atcgaagttt tttgaagcgc
     1321 cgtatctaaa tccgtttggc ctaaaataat atcggtaaga cccggttgct cctgtatatc
     1381 aaataacata tgatgcttgg ccttacgtag atccgtatcc actatacaaa cgcgatgccc
     1441 catatccgca aacgaaatag ccaaattgac cgccgtggtt gttttacctt ctccaggacc
     1501 cgcactcgcc acaagcaacg agcgaatcgg cgaatcaata ttggcaaact gaatatttgt
     1561 acgcaatgaa cggtagcgct ccgatacaaa cgaacgcgga ttaaactgtg aaatcatcgc
     1621 tttccgacga atttcattct tatcttcttc ctcatctttg gttttgattt tattgatttt
     1681 tttccgcaaa ccctcgacgg cattatcttt atcgcctaca ttgatcggag gtatacgtcc
     1741 gacaacagcg atcgaagtaa ccgaactgat cgcggcaatc gatttgattc gattatccat
     1801 aaattctaag cctaccgcaa tggcaatacc tatgattaaa cccagcacgg ttccgacgaa
     1861 aatattggta aataccttat tatatattgc taccggttcc ttggcgggat cgataatttt
     1921 caggctccct gtactgccgt aatactgaat cgacaaactt tctttttgtt ttatcagcga
     1981 actcaggcgt tcttcataaa cctgtttcaa gcgtaataaa cgtgaaatat caattgcatc
     2041 ttggagcagg ttagggtttt ttctttcaaa gtttgttata agcccgctaa acaaacgcgc
     2101 ctgacctttg agtaaaaaaa gttcaatttc ttccgtgttt tttttgtccc atatcgtctt
     2161 ccacacctga ctcatatcac cgctgaccgt attatctgaa ccggttttaa cggacgacag
     2221 cagcgtgtaa tactgactac gcaggtcagc aatttgacga tctatccgct gtacctcggg
     2281 attttctacc gcatccgaac caagcttttc atataaaacc gccttttgcg acgtcaactg
     2341 ttcaatctgt aacttgattt gtttttcttc cgtggattcg ttagccgcat aagaagacga
     2401 ccctgtcagg cgtcgctgaa tcgaatccaa ttgtgaatta agtgcgttaa tattggagct
     2461 tcggatttgt atttgcgttt caaattcagc taatttttcc ttcatgatca gtactttagc
     2521 cagcggacct tccgattgtt tgatgttaac atctgtcttt ttttgtaacg cctgaagctc
     2581 cacttctgtt ttttcaatat tatttctact tacttccact tgttcgtcaa taaactcgat
     2641 actccgctga agcccttccc gatccagttc ggtcacgcgc gacttcaaaa cgtccgttgt
     2701 cacttcaatc atttttttaa ccaaaaacga gtcataattc accgccgata tttcgataaa
     2761 ctgttctact tctttatatg gtttcaatgt cacctgagtt gatgatacgt ttttgagcgc
     2821 caaaacagtt tccgcatcat tacgttcgct caatacgaca cgttcgagct ccttacgaaa
     2881 ccgctgatta aagagatcgg ttgttatgat gacttcataa taaccgaaat cctgcgatcc
     2941 taaggcaatt ttttgctgtt ggggaacatt agcaaaagcc agattggaca atccgttatc
     3001 aaaaaataaa agagccgaag agcgatataa ggtcggttta ctcaacgagt aatacgatac
     3061 tataccggaa aacacgatga aaaccgtaag tacaatccac ttgtgcatca atacagtttg
     3121 caggatcgcg cgcgcgtcaa tcccttgctt atcatcgttt aagtcactca tagatgtcct
     3181 gtactttcta tgtggtatta caataaacca gcttgtttgg aaaggaaaat caaggttaag
     3241 atcgtcgccg cataactcaa cacttgaaat atcgttttca gcgtaaactt tttcgccggc
     3301 acaaatacaa tatcgccggg ttgcacgagc ggaaccgaac cacctttggc cttgagaacc
     3361 gcttctacat caacgatttc ttccgtttga gcgactccga tacgacgtat caatttgaca
     3421 gtgcgaatcg aagcatcgtc tttgggaccg cccgccaaaa aaatcacatc caccagattc
     3481 gcaccgggaa ataattcgta attaccgggt tttcccacag caccgagtac attagcatat
     3541 actgcaccat aaatagacgg aacaaaaata acatcaccgt cacgcaaagc aggcagtttt
     3601 ctgatatcgc cagtttctac tatgatggaa accatgggca cttcgatctc ttcgcgcaaa
     3661 cttgtttcgg gattgatccg gataagtttc gttcgatcta aatcactgcg cacagtcgcg
     3721 ccgcccgccg tcgcaatagc accttgaata gtgtggttac ggggaatttt gtgcgaaccc
     3781 ggctgagcaa cagagccgag aacaatcact tcgatcattt catctttagc ataggccacg
     3841 atcacttcgg cgcgaccgcc gatatattgc gagagccggg ccgataagat attcgtcagc
     3901 tgttcgacgc tcataccgtc cactttgata tccttcataa aaggatattt gatggtacca
     3961 tcggcttcca ctttaatttg tttgggcaac tcgtcgtgac cgtagaccac gacatccaga
     4021 atatcacccg gtctgatttt gcgatcagcg cgtatttgcg ataacgcatc cgctttgagc
     4081 gaatcttttg tttgtgcgtg aagctcaccc gcactactgc tcatggacat tac
//
LOCUS       DAOQNQ010000285         4128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_9736_length_4128_cov_5.203781, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000285 DAOQNQ010000000
VERSION     DAOQNQ010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9736_length_4128_cov_5.203781"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..2893)
                     /locus_tag="PK754_13435"
     CDS             complement(17..2893)
                     /locus_tag="PK754_13435"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNB10553.1"
                     /translation="MNRFAFINRITAAIVFAVAFLGYLFTISPTLSYWDCGEFAATAY
                     TMAIPHPPGSPLFLLLGRIFSILPTSGIGHALGLAVTDYDIAFRINLISTFTSAFAVL
                     FLYLTTVRLILQWKERPESTFGSLKIVIASAISALTFAFTYSQWFNAVESEVYAASIF
                     CTAIVTWLITVWLEKPDDIHSDVYLLLIAYILGLAIGIHLLVILALPFIFFLIYSKKF
                     EITFSGFFKFLLIGLIAMGVIYKVFLFYSIQIPFMLDRFGLSTVSVFLIFAVLIYLSF
                     IMIRNNNHNGALIVISSLLIFLGYSTYATIMIRSGLNPNVDQNDPDTWSKFISYLNRE
                     QYGELVLWPRNAPIWEYQIKKMFVRYFNWQFIGRPDEYALSLIDHLRNAIGWSVDKLQ
                     DTQEDRYSYMYTVLSFRGLYALPFLIGIFGMIHHFSKDWKRALSTLGLFIMTGIMIIV
                     YVNQPDPQPRERDYSYVGAFFAFSIWIGIGIYAIFETIEEKFKDSDMTRMLTYGVTGV
                     AFVLLPLNMFVYNKFSSSRQGNYVAWDYSYNLLVTCEPNAILYTNGDNDTFPLWYLQE
                     VEKVRPDVRIVNLSLVNTEWYINQMKNLEPTYHMPDGTTFKALKVPVGFSDREILGDP
                     KIPNSSIQPMRWDRRDFALDVPKDVYWKDWVESGRKLPPGYDTMTVPKMKFTVEPTIQ
                     GRGLRVQDLMVLDILFANKFERPIYFAITVSNDNMCGLDKYLRMDGLAMKLVGVPGTD
                     MSIDHMYENTFKKYKYRNMNNPNVAYDDNVRRLTQNYRTLYLRMAEYYRTQKNTSSGL
                     HTQIDDAYPAEFTTNQKIVAILDSMEKTIPQNVIPVRDYRLKLAIGQFYSDAGKPEKL
                     REAAEDILANEKAVRLDPSGKLRVAAMFLFNLKDAERAKKILEPMSQADPTNPEILGY
                     YTMALEQLGEYAQAAQVLEQWVAFNPKDVQAQAKLNELKSKIKP"
     gene            complement(2926..3786)
                     /gene="rfbD"
                     /locus_tag="PK754_13440"
     CDS             complement(2926..3786)
                     /gene="rfbD"
                     /locus_tag="PK754_13440"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNB10554.1"
                     /translation="MTILLWGGSGQLGKDIQRVLADQHHVIAISRSECDISDSAQVLS
                     ATEKHRPDAVVNCAAMTDVPGCEQRTEEAFTVNALAARHIADASRILNAYLIHISTDY
                     VFDGNKKTPYIESDLTAPVNTYGITKLAGDHFVNAYHPAAAVVRTSGLYGIFECRGKK
                     TNFVETMLRLGREREIVKVVDDEVLTPTYTHDLAGQIALMLNDKPAGIFHATNAGACS
                     WYDFTQTIFSIAGYTTRLERTTVAAFGSPVRRPAYSVLENAALQKLGIDRMRPWQDAL
                     RAYMTERSDK"
     gene            complement(3807..>4128)
                     /locus_tag="PK754_13445"
     CDS             complement(3807..>4128)
                     /locus_tag="PK754_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB10555.1"
                     /translation="NHRNSLVVLIKNLPRRSFWRTLLFRFVLDGIAAIQSLFRGEPLR
                     MWAVLKAHFWIYAHCISLSSKRKFAKNLHKTDETSFAHLIYPRSIVADYFLKKKREFH
                     TMGF"
BASE COUNT         1144 a          944 c         1014 g         1026 t
ORIGIN      
        1 tgtaacaggc atggtcttac ggtttgattt tggatttcag ctcattgagc ttagcttgtg
       61 cctgtacatc tttcggattg aaggctaccc actgttccaa aacctgagcc gcttgcgcgt
      121 attcgccaag ctgttcgagc gccatcgtat aatacccgag aatttcgggg ttggtcggat
      181 cggcctgcga catcggctcg agtatttttt tagcgcgttc cgcgtcctta aggttgaaaa
      241 ggaacatggc ggccacacgc agtttaccgg acgggtccag acgcacggct ttctcgttag
      301 cgagaatatc ttcagcggcc tcacgcaatt tttcaggttt gccggcatcc gaataaaact
      361 gaccgatcgc cagtttcaga cggtagtcgc gcacagggat gacattctgt ggaatggttt
      421 tttccatcga atctaaaatc gcgacaattt tttgattggt cgtaaattct gccggatacg
      481 cgtcatcaat ctgcgtatgc aatccggatg atgtgttttt ttgcgtacga tagtattcgg
      541 ccatacgcag gtacagcgtg cggtagtttt gcgtcaatcg ccgaacgtta tcgtcatatg
      601 ccacgttcgg attgttcatg ttacggtatt tgtatttctt gaatgtgttt tcatacatgt
      661 gatcaataga catatcggtg ccgggcacgc cgacaagctt catcgccagg ccatccatgc
      721 gcaggtattt atccagaccg cacatattgt cattgctaac ggtgatagca aaataaatcg
      781 gacgttcaaa tttattagca aaaagaatat ccagcaccat cagatcctgc acgcgtaacc
      841 cgcgaccttg gatggtcggt tcgacggtga atttcatttt aggaacggtc atcgtatcgt
      901 aaccgggggg aagcttgcgg ccggattcga cccaatcttt ccagtacacg tctttgggaa
      961 catcgagcgc aaagtcacgg cggtcccagc gcatgggctg gatggatgaa ttgggaattt
     1021 tggggtcacc caaaatttcg cgatcactaa atccgacagg tactttgagc gccttgaatg
     1081 tcgtaccatc cggcatgtgg tatgtcggtt caagattctt catctggttg atataccatt
     1141 ccgtattgac gaggctcaga ttgacgatac gtacatcggg gcgcactttt tcgacttcct
     1201 gtaaatacca aagcgggaag gtatcgttat cgccgttcgt gtagagaatg gcatttggtt
     1261 cgcatgtcac caataagttg tacgaataat cccaggccac atagttgccc tgacggctgg
     1321 aagaaaattt gttgtataca aacatgttga gcggcagcag tacaaaggca acaccggtta
     1381 cgccgtatgt cagcatgcgc gtcatgtcgc tgtctttaaa tttttcttcg attgtttcaa
     1441 agatcgcata aataccaatg ccgatccaaa tcgaaaatgc aaagaacgcg ccgacatacg
     1501 aatagtcacg ttcgcgtggc tgaggatccg gttgattgac atacacaatg atcataatac
     1561 cggtcatgat aaaaagtccc aacgtcgaga gtgcacgttt ccagtcttta ctgaaatgat
     1621 gaatcatgcc gaaaataccg atgaggaacg gcaaggcata taatccgcgg aagctcaaca
     1681 cggtgtacat ataactgtaa cggtcttctt gcgtgtcttg caatttatcc acactccaac
     1741 cgatggcatt gcgcaggtga tcaatgaggg acagcgcgta ttcgtccggg cgaccgatga
     1801 attgccagtt aaaataacgg acaaacatct ttttgatctg atattcccag atcggtgcgt
     1861 tacgcggcca cagcacaagt tcaccgtatt gctcacggtt gaggtagctg atgaatttgc
     1921 tccacgtatc agggtcattt tgatccacat tggggttgag tcctgagcga atcatgatcg
     1981 tcgcatacgt cgaatatccg agaaaaatca atagcgatga aatgacgatc agtgcgccat
     2041 tatggttatt attgcggatc atgataaacg aaagataaat cagcacggca aagatcagaa
     2101 acacggaaac cgtgctcaga ccaaaacgat ccagcataaa agggatttga atagaataga
     2161 aaagaaatac tttgtaaatc acgcccatcg cgatgaggcc gatcagtaaa aatttgaaaa
     2221 aaccggaaaa agtgatttca aattttttgg aataaatcag gaagaaaata aatggcaacg
     2281 caagaatcac caaaaggtga atacctatgg cgagcccgag gatataggcg atcaggagga
     2341 gatatacgtc gctgtggata tcatccggct tttccagcca caccgtgatc agccatgtca
     2401 ctatcgccgt acaaaaaatc gaagccgcat agacttccga ttcgaccgca ttaaaccatt
     2461 ggctataggt aaatgcaaac gtaagtgcac tgatggccga agcgatgacg attttcagcg
     2521 aaccaaacgt tgattccggg cgctctttcc actgtaggat caggcgtact gtcgtcagat
     2581 acaaaaacaa tacggcgaaa gcactggtaa atgttgaaat cagattgatg cgaaaagcaa
     2641 tgtcgtaatc ggtgacggct aaacccaatg cgtgaccgat accggaggtc ggcagaatgg
     2701 aaaaaattcg acccagtaaa agaaacagag gcgagccggg cgggtgggga atagccatag
     2761 tgtatgccgt tgcggcaaat tcgccgcaat cccaatacga cagtgtcggc gaaatcgtaa
     2821 aaagatagcc aaggaaagcc accgcaaata cgattgcggc agttatgcgg ttgataaaag
     2881 caaagcggtt cattctgtgt tagcgctcct ataaatgaat aaatattatt tgtcggatcg
     2941 ttcggtcata taagcgcgca aggcgtcttg ccaggggcgc atacgatcta tgccgagttt
     3001 ttgtaatgcg gcattttcta aaaccgaata cgccgggcgg cggacaggac tgccgaaggc
     3061 cgcgaccgtc gtgcgctcca agcgcgtcgt ataaccggct attgaaaaaa tagtttgcgt
     3121 aaaatcgtac cacgaacaag cgccggcatt agtcgcatga aaaattcccg ccggtttgtc
     3181 gttcaacatc agggcgattt gcccagccaa gtcgtgtgtg taggtcggcg taagcacttc
     3241 gtcatccacg acttttacga tctcgcgttc acggccaagc cgaagcatgg tctcaacaaa
     3301 atttgtcttt tttccgcggc attcgaatat cccgtacaat cccgatgtgc gcaccactgc
     3361 agcagccgga tgataggcgt tcacaaaatg atcgccggcg agtttggtga tgccatacgt
     3421 gttaaccgga gccgtaagat cggattcgat atacggagtt tttttattac cgtcgaaaac
     3481 ataatcggtg ctgatgtgta tcaaataggc attcaaaatt cggctggcat ccgcaatatg
     3541 ccttgcggca agcgcgttga cggtaaaagc ttcctccgta cgttgttcgc aaccgggaac
     3601 atccgtcatg gcggcgcaat taacgaccgc atccggacga tgtttttctg tggcgcttag
     3661 aacctgtgcg gaatcggaaa tatcacattc cgaacggctg atcgctataa cgtgatgctg
     3721 atcggctaat acacgttgaa tgtctttacc cagctggccg cttccgcccc aaagcaatat
     3781 cgtcatgaaa aaaccattat ccttaatcaa aaacccatag tatgaaattc gcgtttcttc
     3841 ttcaagaagt aatccgctac gatgctgcga gggtatatga gatgagcgaa agaggtttcg
     3901 tcggttttat gaagattttt ggcgaactta cgtttactgg acaatgaaat acaatgcgcg
     3961 taaatccaaa aatgcgcttt gagaacagcc cacatacgga gcggctcacc gcgaaaaagg
     4021 ctttggatgg ccgcgatacc gtccagaaca aaacgaaaaa gtagggttcg ccagaaactg
     4081 cgccgaggta aatttttgat caatacgaca aggctgttgc ggtgattc
//
LOCUS       DAOQNQ010000286         4110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_9806_length_4110_cov_5.263872, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000286 DAOQNQ010000000
VERSION     DAOQNQ010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9806_length_4110_cov_5.263872"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PK754_13450"
     CDS             <1..452
                     /locus_tag="PK754_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNB10556.1"
                     /translation="DLMFITSHINFFFANPLQGPNDETIGPRFPDMESEYDREYISIA
                     EKTALKLGVRTQRGVYCAGSGPTYETRAEVKMFQKIGADAVGMSTVPEVIVAKHQNMR
                     VLGISCITNLGTGISQTKLSHEEVTETANRVKTQFQQLITDVVGQIG"
     gene            456..1592
                     /gene="alr"
                     /locus_tag="PK754_13455"
     CDS             456..1592
                     /gene="alr"
                     /locus_tag="PK754_13455"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNB10557.1"
                     /translation="MDTIYHNILKEIRPTHAVIDLAAIAHNMAQIRTRVGSRKIMAIV
                     KANAYGHGICEVTQKAVRSGADYIGVGFLEEALFLRQEDIEAPILVMGGILGYQVKKF
                     VQNDLEITVSSLELAHHINDEIKNQKARVHLKIDTGMERIGVTYKQAVEFVRRVAMLP
                     HIEIVGLYSHFATADEENLFFAHTQFERYCEIIDALKKLGIEIPIKHIANSGAVMNLD
                     STYVDMVRPGISLYGMYPSRFVRRDIQLQPALSLKSKVVFIKRVPAGTGISYGHKYIT
                     PQDCNIVTVPIGYGDGYTRLLTNKADVLIRGKRYPVVGAVCMDQLMVNIGDDRVEIGD
                     EVILIGRSGNEEITAMELAEKIGSIPYEITCMINARVPRIHIHK"
     gene            1594..2757
                     /locus_tag="PK754_13460"
     CDS             1594..2757
                     /locus_tag="PK754_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNB10558.1"
                     /translation="MQKTKARIYLDHSATTPVDKRVVDAMLPFFTEEFGNPSSIYGLG
                     RSAKVAIEDAREKVASVIKAQPSEIVFTGGGSESDNMAIVGAVKSMSAKGKHIITTRT
                     EHHAVLHTFESLEKSGYAVSYVPCDQFGYVSAETIAQAIRPDTVFISVIHINNEVGTI
                     NPIEEISALAKSHQILFHTDAVQSFGKIPFDVKNLPVDLLSVSAHKIYGPKGVGCLYI
                     RRGVKIPSFIEGGSQERGQRAGTENVPGIVGFGKAAEICKDSMSGESERLKKLMTVFW
                     KTLQDIAPQARLNGHPEKRLPGHFNISFPGIGGDEIAMMLDLEGISVSTGSACTSGAV
                     SGSHVLKAMGLGPEFYGSAVRFTFGRLNSEQDIPSVMKALQRIVEKNKNASIL"
     gene            complement(2747..>4110)
                     /locus_tag="PK754_13465"
     CDS             complement(2747..>4110)
                     /locus_tag="PK754_13465"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNB10559.1"
                     /translation="IQSLPFIREIRPVRARSVIASTSSHASSFSSIAASFSRFSYGAS
                     LRQNAFVNAVAAHDSGYSGKGVRIGMIDTGFNLTHTAFSGIHVVADSDFVDHDGNVSE
                     IGSKFTSSHGTFVLSAIASFHESFLIGTAFGADYILARTENNSDAIYELADESRWIAA
                     LEWMEQIGADIVSSSTSFFDEFPGTSNDYTIRDLDGKTGLTTLATDIAFKKGLLVFNS
                     SGNNGAEDQDQYRVSTPSDGKHMIAVGATIGDSTVASFSSRGPTADGRKKPDVAALGY
                     EIRVVSSGTTGYQNFSGTSLSTPIVAGIAALALEANPAWSGSELYDAVRKTASLSKKP
                     NHTIGYGVPDAMKIIRYNPASSDDFNPIRDVVNYPNPANPSTTIRFRSVISGRYSVHI
                     YNTLGALVRTIRDNASITAGETVAALWDLKNDHGRRVSSGVYIYRIAMDDKSETRKIL
                     IIK"
BASE COUNT         1152 a          904 c         1004 g         1050 t
ORIGIN      
        1 gtgatctgat gttcattaca agtcatatca attttttctt tgccaatccg cttcaaggcc
       61 caaacgatga aacgataggg ccacgatttc cggatatgga aagcgaatac gatcgtgaat
      121 atatatctat agccgaaaaa acagcgttga agttgggtgt tcgtacccaa cgcggtgtct
      181 attgtgcggg gtccggaccg acatacgaaa cacgcgcgga agtgaaaatg ttccaaaaaa
      241 tcggagccga tgctgtcggt atgtccacgg tgccggaagt aatcgtcgca aaacatcaga
      301 atatgcgcgt gctggggatc agttgtatta ccaatcttgg tacgggtatc agtcaaacca
      361 aactgagtca tgaagaagtg acggagacgg ccaaccgcgt caaaacacag tttcagcagt
      421 tgataacgga tgtagtcggc cagatcggtt aaacaatgga tacgatatac cacaatatac
      481 taaaagaaat tcgccctacg catgcggtga ttgatttggc agcgatcgca cacaatatgg
      541 cacagatcag aacccgggtc ggcagccgaa aaatcatggc tatcgtcaag gctaacgctt
      601 acggtcacgg tatctgcgaa gtgacgcaaa aagccgtgcg gagcggggcg gactacatag
      661 gtgtgggctt tctcgaggag gcgctttttt tacggcaaga ggatatcgaa gcgcctatcc
      721 tggtgatggg cggtattcta ggttaccagg ttaaaaaatt tgttcaaaac gatctcgaaa
      781 tcactgtttc ttctttggaa ctggcgcatc atatcaacga cgaaattaaa aatcaaaaag
      841 cgcgggttca tcttaaaata gataccggca tggaacgaat cggtgtgaca tacaaacaag
      901 cggtagagtt cgtacgacgt gtggccatgc ttccgcatat tgagattgta ggtctctatt
      961 ctcattttgc gacggcggat gaagaaaacc ttttttttgc gcatacgcag tttgaacgat
     1021 attgcgaaat catagatgca ttgaaaaaat taggtattga aatcccaatc aagcacattg
     1081 ccaatagcgg agccgtgatg aaccttgatt ctacgtacgt ggacatggtg cgtccgggca
     1141 tttcgttata cggcatgtat ccttcgcgtt ttgttcgtcg cgatatacag cttcagccgg
     1201 cgctttcttt aaaatctaaa gttgttttta tcaaacgtgt gccggccggt acgggcatca
     1261 gctacggcca caagtatata acgcctcagg attgcaatat cgttacggtt ccgatcggat
     1321 acggcgacgg atatacacgt cttttgacga ataaagccga tgtgttgatt cgcggtaaaa
     1381 ggtatcctgt ggtcggtgcg gtgtgcatgg accagttgat ggtcaatatc ggcgatgatc
     1441 gtgttgaaat aggggatgaa gtcattctga tcgggcgttc gggcaatgaa gaaattacgg
     1501 cgatggagtt agctgaaaaa atcggttcga ttccttacga aattacctgt atgatcaacg
     1561 cacgtgtacc gcgtattcac attcacaaat aatatgcaaa aaaccaaagc acgtatctat
     1621 ctggatcaca gcgcgacaac accggtagac aagcgggtcg ttgatgcgat gctccctttt
     1681 ttcaccgaag aattcggtaa tccatccagt atttacggac tagggcgctc ggctaaagtg
     1741 gctatcgaag acgcgcgcga aaaagttgcg tcggtaataa aggcacaacc ttcagagatt
     1801 gtttttaccg gcggcggctc cgaatccgac aatatggcca ttgtcggcgc agtaaaatca
     1861 atgagtgcca agggtaaaca tattattacg acacgaacgg aacaccatgc cgtcctgcat
     1921 acgttcgaat cgttggaaaa atcgggttat gctgtgtcct atgttccatg cgatcagttc
     1981 ggatacgtgt ccgcagaaac tatagcacag gccattcgtc ccgataccgt attcatatct
     2041 gtcatccaca tcaataatga agtaggtacg atcaatccga tcgaagaaat aagcgcttta
     2101 gccaaatcgc atcaaattct tttccatacc gatgcggtgc aaagttttgg aaaaataccg
     2161 ttcgatgtaa aaaatttacc agtggatttg ttatctgtct ccgcccataa aatatacggc
     2221 cccaaaggtg tgggctgtct ttatatccgg cgtggcgtaa aaataccttc atttatcgaa
     2281 ggcggttcac aagagcgtgg gcaacgtgca ggcacggaaa atgtgccggg tatcgtcgga
     2341 tttggcaaag ccgcagagat ctgcaaagac agtatgtccg gtgaatcaga acgtctgaaa
     2401 aaactaatga cagtattttg gaaaactctg caagatattg caccgcaagc ccggttgaac
     2461 ggtcatcctg aaaaacgatt gccgggacat tttaatattt cgtttcccgg gatcggagga
     2521 gatgaaatag ctatgatgtt ggatttggaa ggtatttccg tttctacggg atcggcttgc
     2581 acatccggtg ccgtaagcgg gtcgcatgtg ctcaaggcga tggggttagg tcctgaattt
     2641 tacgggagtg cggtgcgttt cacattcggg cgtttgaatt ctgaacaaga tattccatca
     2701 gtgatgaagg cgcttcagcg gatagtagaa aaaaacaaaa atgcttctat tttataataa
     2761 gaatttttcg tgtttctgat ttgtcatcca ttgcaatgcg gtaaatatat actcccgaac
     2821 tgaccctccg tccgtgatca ttttttaagt cccataacgc cgctaccgtt tctcccgccg
     2881 tgatagacgc attgtcccgt atcgtgcgca ccaatgcacc aagcgtatta tagatatgta
     2941 cagaataacg tccgcttata acggatcgaa aacgtatagt agtggatggg ttggccggat
     3001 tgggataatt gacgacatcg cgtatcggat taaaatcatc ggacgaagcg gggttataac
     3061 gaattatctt catcgcatca ggcacaccat agccgatagt atggttaggt ttttttgata
     3121 aactggccgt ttttcggacg gcatcatata attccgaacc ggaccaggcc gggtttgctt
     3181 ccaaagctaa ggcggcaatt ccggctacaa taggggttga tagcgaagtg cccgaaaaat
     3241 tttggtaacc cgttgttccg ctggaaacta cgcgtatctc gtagcccaac gctgcgacat
     3301 ccggtttttt tcgaccgtca gccgtaggcc cacgcgaact aaaactggct accgtgctgt
     3361 cgccgatcgt cgcaccgaca gcgatcatat gcttcccgtc ggatggcgtg ctgacgcgat
     3421 attgatcttg atcttcagcg ccattatttc cggaactatt aaatacgagc aggcctttct
     3481 tgaatgcaat atcagtagca agtgtagtca agccggtttt tccgtctaga tcgcgtatgg
     3541 tgtaatcatt tgaagtgcct ggaaattcgt caaaaaaact ggttgaactg gaaactatat
     3601 ccgccccgat ttgttccatc cattccaaag cggcaatcca gcgtgattcg tccgccaatt
     3661 catagattgc atcactatta ttttcagtac gagctaatat ataatccgca ccgaaagccg
     3721 taccgatcaa aaaactttcg tgaaaacttg caattgcaga aagtacaaat gtcccatgac
     3781 tgctggtaaa tttggaaccg atttcgctca cgttaccgtc gtgatccaca aaatcggaat
     3841 ctgctacgac gtgaattcct gaaaaagccg tgtgggtgag attaaaaccc gtatcaatca
     3901 tgccgatgcg aactcctttc cctgaataac cggagtcatg agcggcaacg gcattgacaa
     3961 atgcattttg ccgtaatgac gcgccatacg aaaagcgact aaacgaggct gcgatagacg
     4021 aaaaagatga tgcatgtgaa gaagttgacg ctatcaccga tcgggctcta accggacgaa
     4081 tttcgcggat aaacggaagc gattgaataa
//
LOCUS       DAOQNQ010000287         4103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_9829_length_4103_cov_5.832263, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000287 DAOQNQ010000000
VERSION     DAOQNQ010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9829_length_4103_cov_5.832263"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..377)
                     /locus_tag="PK754_13470"
     CDS             complement(3..377)
                     /locus_tag="PK754_13470"
                     /inference="COORDINATES: protein motif:HMM:NF015945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HNB10560.1"
                     /translation="MGIFTRMADIVKANINDFLDKAEDPEKMIKQMVIEMEEAVNKAT
                     TAVGTAIANQKRLEKQFNENKKLADEWQNKAVQAVNAGRDDLASQALAKKNSYATAAA
                     SLEPTLQQATATAGKHGQVGQP"
     gene            complement(431..1543)
                     /locus_tag="PK754_13475"
     CDS             complement(431..1543)
                     /locus_tag="PK754_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HNB10561.1"
                     /translation="MNKPAFTIGIEEEFQTVDPETRELRSHVSGQMIEEGKLLLSEQI
                     KMEMHQSVVEVGTNICQNITEAKADVIKLRRTIHQLATKRGLGIVAASTHPFSDWKTQ
                     PITEHERYKEIVENMGDVARANLIFGLHVHIGMPDNESCIAIQNQIRYFLPHILALST
                     SSPFWIGRSTGLSSIRTQIFRRFPRTDIPPIFDSWSDFEDYVNLLIQTKCIDNGKRIW
                     WDARPHPSFGTLEVRICDLPTRVDETIAIAALIQALMHTLYRTYKKNMSWRIYPNQLI
                     SENKWRASRYGLHGKLIDFGKKEEVPTKELIYELIRFVDDSVEELGSRNEINYIYKIL
                     DGGTSSDRQLAVYEQSGRDLKAVVDFLLRDTLEGLY"
     gene            complement(1540..2715)
                     /locus_tag="PK754_13480"
     CDS             complement(1540..2715)
                     /locus_tag="PK754_13480"
                     /inference="COORDINATES: protein motif:HMM:NF025761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNB10562.1"
                     /translation="MAGDELKFVELNAECPAGIAYSDIAAKVYDKLPMIREFKKHFNV
                     EKFTIRQELLNGLLSTYAVWRGNKKNKKPRIAIVDWREVPTFTEFQLFQEFFKSKKLD
                     CIIVDPRDLTYEKGRLMAGKKEIDLVYKRILTNDCVEKPEETKALVQAYRDQNVCMIN
                     PFRAKLVHKKSMFAVLTHEKNQKLFNGEELSVVLKHIPWTRMLRDEITYFNGKQIDLL
                     DYVSTYKNNFVIKPNDEYGGKGVCLGMEATDQQWSAVLHEAKNGEHYVVQELVKIPKQ
                     PFPTVINGNLEFVDMVVDMDPYAFGPNVEGILTRLSASSLANVTAGGGTTPTFVITKK
                     TKKAKKTLKNEIRRFISKQKPKLSYLKTNNKKTKKKVTVKRRSKKIEMINKNKYLII"
     gene            complement(3252..3323)
                     /locus_tag="PK754_13485"
     tRNA            complement(3252..3323)
                     /locus_tag="PK754_13485"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3289..3291),aa:Thr,seq:cgt)
     gene            complement(3408..>4103)
                     /locus_tag="PK754_13490"
     CDS             complement(3408..>4103)
                     /locus_tag="PK754_13490"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB10563.1"
                     /translation="GKRLGRKIALALAETGYDIIINYHQSVVDARKTEKLIRQIGRKA
                     VLIKADVRRPDHIKKMFNEVIKKFGKIDVLINNAAIFPKKTELPNIPESIWDDVIDSN
                     LKSSFLCAQEAAKYMLRQKSGKIINIASLGAFLSWKNYIPYNVSKAGVVMLTRSLAKA
                     LAPDITVNAVAPGTIIVPEEEVLSEHLPTMDRIPMKKYGKPADITAAVLFLLQNEYIT
                     GQIVGVDGGATIP"
BASE COUNT         1106 a          836 c          780 g         1381 t
ORIGIN      
        1 acctaaggtt ggccaacctg cccatgcttt ccggcagttg ctgtagcttg ttgtaatgtc
       61 ggctctaaag aagcggcagc tgttgcatac gagtttttct ttgccaaagc ctgtgaagcc
      121 aaatcatcgc gtccggcatt aaccgcctga acggctttgt tttgccattc atcggctagc
      181 tttttgtttt cattaaactg tttttcgagt cttttttgat tagcgatagc tgttccaact
      241 gctgttgtgg ctttattgac tgcttcttcc atttcgatga ccatttgttt gatcattttt
      301 tcaggatctt cagctttgtc caaaaagtca ttgatattgg cttttacgat atctgccata
      361 cgcgtgaaaa tacccatata acctccaatt atttaataag ttgacattaa tttatgatga
      421 ataaaaattg tcaatataag ccttccaaag tatcgcgtaa tagaaaatct acaacagctt
      481 ttaagtccct gccggactgc tcgtacacag ccaactgccg atcagaactg gtgccgccat
      541 ctaaaatctt ataaatatag ttaatttcat tgcgacttcc aagctcttcg acagaatcat
      601 ctacaaagcg aattagttca taaattagtt cttttgtcgg aacttcttct tttttaccaa
      661 aatcaatcaa tttaccatgt aatccgtacc tactcgcccg ccatttattt tcagatatca
      721 attgattggg atatatgcgc caggacatgt ttttcttgta cgttctgtat aaggtatgca
      781 tcagcgcttg tatcaaagcc gcaattgcta tcgtttcatc tacacgtgta ggcaaatcgc
      841 aaattcttac ctccaaagtt ccaaagctcg gatgaggtcg tgcatcccac caaattcttt
      901 tgccgttatc aatgcatttc gtttgaatta agaggtttac ataatcttca aaatctgacc
      961 atgagtcgaa aatcggtggg atatccgttc gtggaaaacg cctaaaaatt tgagtcctga
     1021 ttgagcttaa gccggtactt cgaccgatcc aaaacggtga acttgtcgaa agtgctaaaa
     1081 tatgaggtaa aaagtatctt atttggtttt gaatcgcaat gcacgattca ttatccggca
     1141 ttcctatatg gacgtgtaat ccgaaaataa gatttgcacg tgccacatcc cccatgtttt
     1201 cgactatctc tttgtatctt tcgtgctccg taataggttg tgttttccaa tcgctaaatg
     1261 ggtgtgtgct ggcagcgacg atacccagcc ctcttttggt ggcaagttga tgaatggtac
     1321 ggcgcagctt tataacatcg gcctttgctt cagtaatgtt ttgacaaatg ttcgttccaa
     1381 cttctactac ggactgatgc atttccattt tgatctgctc ggaaagaagt aatttacctt
     1441 cctcgatcat ttgaccactg acatgggaac gcaattcgcg cgtttcagga tcaacagttt
     1501 gaaactcttc ttcaattcct atcgtgaaag cgggtttatt catattatta aatatttgtt
     1561 tttattaatc atttcaattt tttttgagcg cctttttact gtaactttct ttttagtctt
     1621 tttattattt gtttttaaat aacttaattt aggtttttgt ttggatatga atcttctaat
     1681 ctcatttttt aatgtttttt tggccttttt tgtttttttt gtaataacaa aagtcggagt
     1741 tgtaccgcca ccggcagtaa cattggctag tgaagatgca gataaccgtg tcaatatacc
     1801 ttcaacattc ggaccgaacg cataaggatc catatcaaca accatatcaa caaattctaa
     1861 attaccattg ataacagttg gaaaaggttg ttttggaatt ttcacaagct cctgcactac
     1921 atagtgctcc ccgttctttg cttcatggag cacggcgctc cactgttggt cggtagcttc
     1981 cattcctaaa caaacccctt ttcctccgta ctcatcatta ggctttatga cgaaattgtt
     2041 tttatatgta gatacgtagt ccaaaagatc aatttgtttt ccattaaagt atgttatttc
     2101 atcccggagc attcgagtcc atggaatatg tttgagtacg actgaaagtt cttcgccatt
     2161 aaaaagtttt tgattttttt catgggtcaa aacagcgaac atagattttt tatgaacaag
     2221 tttagcccta aaaggattga tcatacatac gttttgatcg cggtatgctt gaactaaggc
     2281 ttttgtttcc tccggttttt caacacagtc gttggtcaat atccgcttat aaaccaaatc
     2341 tatttccttt ttcccagcca tgaggcgacc tttttcatac gtcagatcac gcggatctac
     2401 gataatacag tctaattttt tagatttaaa aaattcctga aaaagttgaa actcggtaaa
     2461 ggtcggtact tctctccagt ctacaatggc gatgcgtggt ttcttgttct ttttgtttcc
     2521 tcgccaaacg gcgtaggtag ataataatcc gttcagtaat tcctgacgga tggtaaattt
     2581 ttcaacgtta aagtgttttt taaattcgcg tatcatcggt aacttatcat acaccttggc
     2641 tgcaatatcg gaataagcaa tccctgcagg gcattcggca ttgagctcca cgaactttaa
     2701 ttcgtcgccg gccataaatg aatcccatcg tgcggtgatc gaaagcctgt catatcccgg
     2761 gtccatcatt acgagttcgc gttccctatc taaaagaccg gcttgctcca tgatgattgg
     2821 attttcgagc gcggcatttt taaatttcgt aacggcatta cggagtatct tgcaaacgta
     2881 ttggatataa cggtattgtt ctagtgtaat aaaattgggt ctcaggaaac tgtacatatg
     2941 ccgcccgcca aaggtcagtt tttcctttgc acattgcttc agatgttttt caaaaacggc
     3001 ttgaatgcgc cgttcgtgat tttttaccaa tgcgttataa aactgaatgg tatcacttgt
     3061 gttcataaat ggatatatat tatttggaat ggaaaagtgc tgtaaagata aatgcaaata
     3121 aaggtttatt caagaaaatt tgaattaacc ttaaatggga tttggaacga ggtgtttggg
     3181 gataaactaa ttagcgggat acgtgagagc taaaaaggaa aacctgatca catgatgatc
     3241 aggttttgaa gagccacaag cgggattcga acctgcgacc tgctgtttac gaaacagctg
     3301 ctctaccgct gagctattgt ggcaaaatcc ggtcgcaaat atatagaatt ataaataaaa
     3361 atcaataaaa ttttaccttt taaatttttt gtttaacgtc tcaagtatta tggaattgta
     3421 gcccctccat ccacaccgac gatctgaccg gtgatgtatt cgttttgcag cagaaataaa
     3481 actgcggccg ttatatcggc gggcttgccg tattttttca taggaatgcg atccattgtc
     3541 ggtaagtgtt cagacaatac ctcttcttcc ggaactatga tcgtaccggg cgcgacagca
     3601 ttgacagtga tatccggggc caatgcttta gctaagctgc gcgttagcat aactacgccg
     3661 gctttggata cattatatgg gatgtagttt ttccacgata aaaatgcgcc caacgatgcg
     3721 atgttaatta ttttaccgga tttttggcgt aacatgtatt ttgcagcttc ttgcgcacac
     3781 aaaaaacttg atttaagatt cgagtcaatc acatcatccc atatagattc ggggatattt
     3841 ggaagttcgg ttttttttgg aaaaatcgca gcgttattta tcagaacatc aattttaccg
     3901 aatttcttaa tcacttcatt gaacattttt tttatgtgat ccggacgtcg aacatccgct
     3961 ttgatcaaca ccgctttgcg ccctatttga cgtataagtt tttccgtttt acgggcatcg
     4021 acaacggact ggtgataatt gataatgata tcgtagccgg tttcagctaa agccaatgct
     4081 atttttcggc ccaatctttt acc
//
LOCUS       DAOQNQ010000288         4068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_9988_length_4068_cov_4.958634, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000288 DAOQNQ010000000
VERSION     DAOQNQ010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9988_length_4068_cov_4.958634"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PK754_13495"
     CDS             complement(<1..280)
                     /locus_tag="PK754_13495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HNB10564.1"
                     /translation="MIKILAISGSLRKNSYNSALLRAMIKLAPENVSVELFYGLGELP
                     LFNPDIEDQAFPSVLHFRNQLASHDGIVIASPEYAHGVTGAMKNALDWV"
     gene            complement(277..957)
                     /locus_tag="PK754_13500"
     CDS             complement(277..957)
                     /locus_tag="PK754_13500"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HNB10565.1"
                     /translation="MRSPKQLFQILSLLLIAALTAVIFFIHRIHDKSKQISENWLPSV
                     MHVGNLNTLTSDFRIMELQHILSTSDDQMRTYESAMQRIADLINSEMKLYEPLITTSV
                     EKTLYEDFLNRWSEYMKHHIDMLQLSKLNKNEDAKQLIRARSEVLFNEYSSALSALVH
                     ENRRAADDEVKRANALYIGSVINLILILGCVAVFVVISLRQMRKIFDRVLSTAMDVVA
                     KERDGKSI"
     gene            1145..2539
                     /gene="purF"
                     /locus_tag="PK754_13505"
     CDS             1145..2539
                     /gene="purF"
                     /locus_tag="PK754_13505"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNB10566.1"
                     /translation="MSEDIKEECGVFGIYGHPEASRLTYFGLYSLQHRGQESTGIVSA
                     DGDRTYRHVGMGLVSDVFADDKIFDRLPGHSAIGHNRYSTTGGSFMQNVQPLTAIIST
                     GPISAAHNGNLTNYQSLRRELQNNGAILQGNNDTEVILHLAARSGKTTVEEQIIAALH
                     QIRGAYSLLLITKDKLIAARDPWGIRPLCIGKLDQATVIASESCALDIIGAEYVRDVK
                     PGEFIVVDHDRMQTRMIFDPLPTPAHCIFEFVYFSRPDSKIFGDNVDKARRKLGKRLA
                     LEHSVDADIVISVPDSSNTAAVGYSRRTGLKFELGLIRNHYVGRTFIHPVQSMRDLKV
                     RLKFNAVEGVLRDRRVVVIDDSIVRGTTLKALVTMIRKAGAKEVHIRISSPPVTSPCY
                     YGMDFPTKEELIANTMNKDTLCKSIGADSLEYLSLDGMLECVPNGPHNYCTACFSGKY
                     PVPIEPDTQITQCD"
     gene            2743..>4068
                     /locus_tag="PK754_13510"
     CDS             2743..>4068
                     /locus_tag="PK754_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HNB10567.1"
                     /translation="MSEHSSQGVKGLPSNAYTELKPGETYTPIMSPQQVYPEVNFRSV
                     FWGLIMAMLFSAAAAYLGLKIGQVFEAAIPIAIIAVGLSAASKRKNALGENVIIQSIG
                     ANSGAVVAGAIFTIPALYILNLQADFFKVFLASLFGAFLGTLFLIPFRKYFVADMHGK
                     FPFPEATATTEVLVAGEKGGNQAKVLVLSGIIGGIYDFIVATFGWWSEVFSTRALPWG
                     MELADKAKLVFKVNIGAAVTGLGFIIGLRYSLIIACGSFVSWYLLIPTVYYIGAGLTT
                     PLGANATKLIGAMTAEEIFRNYVRHIGIGGIAMAGIIGIIRSSGIIKSAFILGFKEIG
                     GNKGDNANALRTQRDLGMKIVGIGILVVAVMIFIFFQMGVVQNLTHALIGLAVVLIIS
                     FLFTTVAANAIAIVGTNPVSGMTLMTLIIASVVLVSSGLTGEVGMLSALI"
BASE COUNT         1078 a          960 c          870 g         1160 t
ORIGIN      
        1 cgacccaatc caatgcgttt ttcatcgctc cggttacacc gtgtgcatat tctggactag
       61 caataactat cccgtcatgg gaagctaatt gattacggaa atgtaaaact gaagggaacg
      121 cttgatcttc aatgtctgga ttaaatagcg gcaattcgcc gagaccgtaa aacaactcta
      181 cagacacatt ttcaggcgct agttttatca tagcccgtag taacgcgctg ttatacgaat
      241 tttttctaag gctccctgaa atggctaata tttttatcat atgcttttcc catcgcgctc
      301 tttggctact acatccattg ccgtagataa aacgcgatca aagatttttc gcatctggcg
      361 taatgatatg acaacaaaaa ccgcaacgca ccctaaaatc aaaattaaat tgataaccga
      421 cccgatatat aacgcgttag cacgtttgac ttcatcgtcg gcggctctgc gattttcgtg
      481 cacaagcgcc gaaagtgcag acgaatactc attgaataaa acttccgaac gcgcacgaat
      541 caactgtttt gcatcttcgt ttttgttgag tttggacagt tgaagcatat cgatatggtg
      601 cttcatatac tcagaccatc tattcaaaaa atcctcatac agagtttttt ctacggacgt
      661 cgtaatcaga ggttcataca gtttcatctc cgagttaatc aaatcggcta tgcgttgcat
      721 agctgattca tacgtacgca tctgatcgtc tgaagtggac aaaatatgct ggagctccat
      781 aatccgaaaa tcggacgtga gtgtgtttaa gttaccgaca tgcattacgc tgggcaacca
      841 attctcgcta atctgtttgg atttgtcatg aatacgatga ataaaaaaaa ttaccgccgt
      901 aagcgccgca atcaataaca atgaaagtat ctgaaatagc tgtttgggtg aacgcatgct
      961 gattctccga aatatcaacg ttttcatatt ccatttacat aaaatacaaa ctttacgtaa
     1021 aaatctctac atgaataatg attgttacat tcgtcaaaaa acattggatg aatgtctttt
     1081 tttttctatc ttgcgccgca cgaattgcgt taagtatgtt atcaaaaatc caataagtaa
     1141 cactatgtca gaagacatca aagaagaatg tggtgtgttt ggtatttacg gccatccaga
     1201 agcatcacga ctgacttatt ttggcttgta ttccctccaa catcgcggac aagaatccac
     1261 cggcatcgta tcggccgatg gtgatagaac ctatcgccat gtcggtatgg gattagtcag
     1321 cgacgtattc gctgatgata aaatttttga tcgcttacct ggtcattcgg ccatcggtca
     1381 taatcggtat tcaacgaccg gcggaagttt tatgcaaaac gtgcaacctt tgacggctat
     1441 tattagcacc ggtccgatat ccgctgccca taacggaaac ctgactaact atcaaagtct
     1501 tcgccgcgaa ctccaaaata acggagccat ccttcaagga aacaacgaca ccgaagtcat
     1561 tcttcatctc gctgcacgtt ccggtaaaac cactgtcgaa gaacaaatta tcgccgcatt
     1621 gcatcagatt cggggcgcat attcgctact tctgataact aaagacaaac tcattgctgc
     1681 acgtgatccg tggggtatcc gtccgctctg tatcggaaaa ttggaccaag ctaccgtaat
     1741 agcaagtgag tcctgtgcgt tggacatcat cggcgccgaa tatgtgcgtg acgtaaagcc
     1801 gggcgaattt atcgtcgtgg atcatgaccg tatgcaaaca cgaatgattt ttgacccgct
     1861 acccacaccg gcgcattgta tttttgaatt tgtttatttt tctcgtcctg acagtaaaat
     1921 ttttggcgat aatgtcgaca aagcacggcg taaactcgga aaacgtctcg ctctggaaca
     1981 cagcgtggac gcggatatcg tgatcagtgt accggactcc agcaataccg cagccgttgg
     2041 ttattcacgc cgaacgggat tgaaatttga actcggtctt attcgtaacc attatgtagg
     2101 ccgcaccttc attcatcccg tacagtctat gcgcgatctg aaagtgcgat taaagtttaa
     2161 tgcggtagaa ggcgttctgc gtgatcgccg tgttgtcgtc attgacgatt cgatcgtgcg
     2221 cggcactacg ctcaaagctt tggttacgat gatacgtaaa gccggggcca aagaagtgca
     2281 cattcgtatc agttccccgc ccgtcacttc accatgttac tatggcatgg atttcccgac
     2341 caaagaagaa ttgatcgcca ataccatgaa taaagataca ctctgcaaaa gtatcggcgc
     2401 cgattcgttg gagtatcttt ctcttgacgg tatgttggag tgtgtgccca acggcccaca
     2461 caactactgc acagcttgct ttagcgggaa atatcccgta cccatcgaac ccgatacaca
     2521 aatcacccaa tgtgattaac cgcaagcctt atatattcgc agttggcctt tatgattttt
     2581 ctcatggtat tctgcgcttg gcgactgtaa cctttctttg atttacgttt gacctgatta
     2641 cttcagatag gctcgtggtt gcttgttttg agcgcctatc tatattcaat caatcatttc
     2701 taaataatta aattctctca ccgaacttca agcgagacac gtatgagcga acattcttct
     2761 caaggcgtca aaggtttacc ctccaatgcc tacacggaat taaagcccgg cgaaacctac
     2821 acccccatta tgtcaccgca gcaggtgtat cccgaagtaa atttcaggtc tgttttttgg
     2881 ggactgatca tggcgatgtt gttttctgcg gccgccgcat atttaggact caaaatcggt
     2941 caggtttttg aagccgccat ccctatcgcg atcatcgccg taggactttc cgccgcttcg
     3001 aaacgcaaaa atgcactggg tgaaaatgta atcattcaat ccatcggagc caatagcggc
     3061 gcggtggtag caggcgccat atttacaata cctgcgcttt acattctcaa cctgcaagct
     3121 gactttttca aagtattcct agcatcgctt ttcggtgctt ttctaggaac cctattcctg
     3181 attccattcc gtaaatattt cgtagccgac atgcacggta agttcccctt tccggaagca
     3241 acggccacaa ccgaagttct tgtggccggc gaaaaaggcg gcaatcaagc taaagtattg
     3301 gtattatcag gcatcatagg cggaatttac gattttatcg tggctacttt tggttggtgg
     3361 agcgaagttt tttcaacccg cgcattgcca tggggcatgg aattagccga taaagccaaa
     3421 cttgttttta aagtgaatat cggtgccgcc gtaaccggac ttggttttat tatcggtctg
     3481 cgttattcat tgatcattgc ttgcggctca ttcgtctcgt ggtatcttct gataccgacc
     3541 gtatattaca taggcgcagg ccttacgaca ccgctcggtg ccaatgcaac caaactgatc
     3601 ggtgctatga cggcggagga aatttttaga aattacgttc gtcatatcgg catcggcggt
     3661 atcgcaatgg ccggcatcat tggcattatt cgttcatccg gtatcatcaa gtcagctttc
     3721 atcttaggat tcaaagaaat cggcggtaac aaaggcgaca acgctaatgc cttgcgcacc
     3781 caacgtgatc tgggcatgaa aatcgtcggc atcggtattt tggttgtagc cgttatgatt
     3841 ttcatatttt ttcaaatggg cgttgtacaa aatctgaccc acgcactgat cggactcgct
     3901 gtggtactca ttatttcgtt tttgtttacg accgttgcgg caaatgcgat cgccattgtc
     3961 ggaacaaatc ccgtttccgg tatgacctta atgacgctta tcatcgcgtc tgtagtatta
     4021 gtttcatccg gccttacagg tgaagtcggt atgctctcgg cactgatc
//
LOCUS       DAOQNQ010000289         4052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_10069_length_4052_cov_5.956467, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000289 DAOQNQ010000000
VERSION     DAOQNQ010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10069_length_4052_cov_5.956467"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PK754_13515"
     CDS             complement(<1..645)
                     /locus_tag="PK754_13515"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNB10568.1"
                     /translation="MAVPLLDLKAQYRAHKSEIDEAMLRVAESQHFIMGPEVEKLEKS
                     LCTYLECKHAIAVSSGTDALLVALMALDIQPGDEVIVPTYSFFATAGVVARLHAKPVF
                     VDSDPLTFNIRASDIEKKITAKTRVIMPVHLFGQSSDMGAIMAVAKKHNLKVIEDGAQ
                     AIGVQYSDGKKVGNFGDIGCFSFFPSKNLGCFGDGGLVTTNDDALGEKLRVLRVH"
     gene            complement(645..1232)
                     /locus_tag="PK754_13520"
     CDS             complement(645..1232)
                     /locus_tag="PK754_13520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNB10569.1"
                     /translation="MSEKKYFVHPLAVVDDNVEIGEGTKVWHFSHIQTGARIGKKCVF
                     GQNVNVANHVKVGNFCKVQNNVSIYEGVELEDYVFCGPSMVFTNILDPRCKYPQVGAE
                     FYIKTLVKEGASLGANSTIVCGHTVGRSALVGAGAVVTKDVPDFALVVGNPARIVGWV
                     SEAGKKLNFDAQGFAYCEKSKKKYKFENGLVKEIE"
     gene            complement(1225..2208)
                     /locus_tag="PK754_13525"
     CDS             complement(1225..2208)
                     /locus_tag="PK754_13525"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNB10570.1"
                     /translation="MDYTKVKLGIIGCGNWGLNHVRTAHGLLGSNLLLTADAKPDAIQ
                     RVHTVSPKIPFTSDIHSLIAHSNINAVVIATPAETHFDLAKRCIENGKHVLIEKPITL
                     ISEHTVELLSLAQKYDVKIMVGHVLLYHPAVLKLKQMMDEGYIGKLQYIYSNRLNLGA
                     IRSEENSLWSFAPHDISIVQYLIGKNPETIEAKGAAFVQKNVEDSTITYLTYPGNIHV
                     HVFVSWLHPFKEQRLVVIGDKGMIVFEDSLKTDKLKFYKKGFQQSNGKIEKFEVDFEV
                     IPFDAAQPLSEEHRHFYDCLLHGKTPRTDGEHALEVLRILEKAQERITQHV"
     gene            complement(2212..3852)
                     /locus_tag="PK754_13530"
     CDS             complement(2212..3852)
                     /locus_tag="PK754_13530"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNB10571.1"
                     /translation="MKKHLFLTMISALILSAMTDTTVLAQQDRSKIKEELEKDNSSGD
                     PDDVLFPEKSNNILETVVDPKQYYVGPGDQFLVSIWGQVNQNFRTVVSPEGFLIVPTV
                     KAIDVKNLNLADVQERVGKSIRTVYTNAEVTINLVSLRSFRVPVGGMCNNPSSVVVSG
                     TERVSTAIAKAGGLNGKASQRNIILYRKDGTSQSVDFIKRGRNLDLDSDPLLNDGDRI
                     FVPAQYASYQILGAVASHGTMEYVANENIIDAIRLAGGLVQGIDSSIFIMYRMAKDTG
                     REVIRITRDLREAYQNPEDTTINLRLKPDDRIYFRKHAQYHDRADVAIQGEVMYPGSY
                     AIIEDKTTVSDVIKRAGGFTPYVSLDRISIYRSPAKEDIDQEYERLKLTPVSEMNDIE
                     KSYFKTKTRQQKPVVQTDFKKLFADGKVNSEYDVVLKRGDYIDVSSIRRTVTVVGGAL
                     LSGIVDFDHGKDYRYYIDKCGGFTDRAKKGDIKVIKTASQKWLDADDDVLIEDGDVIF
                     VPEKEPVDGWQIFKDALTITGQLAAITSTIILIYFTVK"
     gene            complement(3849..>4052)
                     /locus_tag="PK754_13535"
     CDS             complement(3849..>4052)
                     /locus_tag="PK754_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HNB10572.1"
                     /translation="PCLTMRANTERPVTLDVGTNTLVGQDRDALKSCMEAVLNRSYKK
                     GNIPALWDGMAAERIAEKLKDLV"
BASE COUNT         1109 a          981 c          778 g         1184 t
ORIGIN      
        1 atgcacgcgc aggacgcgca acttttcacc gagagcatcg tcattggtcg tcacaaggcc
       61 tccgtcgcca aagcaaccga ggttcttact agggaaaaaa gaaaagcaac caatgtcgcc
      121 aaaatttccg actttttttc catcgctgta ttgtacgccg atcgcctgcg caccgtcttc
      181 gatgactttg agattatgtt tttttgcaac cgccatgatc gctcccatgt cggagctttg
      241 tccgaaaagg tgcaccggca tgataacgcg tgttttggca gtgatctttt tttcaatgtc
      301 agatgcacgg atattaaaag tcagcggatc cgaatcaaca aaaaccggtt ttgcatgcag
      361 tcgggcaacc acgccggcag tggcaaaaaa agaatacgtc ggcacgatta cttcatcgcc
      421 gggctgaata tcgagcgcca tcagcgctac caataacgca tccgtcccgg aagaaactgc
      481 aatcgcatgt ttgcattcca gataagtaca caaagatttc tctaattttt caacttccgg
      541 gcccataata aaatgctgcg attcggcaac gcgcaacatg gcttcatcaa tctccgattt
      601 gtgagcacga tattgtgctt tgaggtctaa aagaggtaca gccattattc tatttccttt
      661 accaaaccgt tttcaaattt atattttttc ttggattttt cgcagtaagc aaaaccctgt
      721 gcatcaaaat ttaatttctt accggcttcg ctgacccaac cgacgatgcg tgccggattt
      781 ccgacgacca atgcaaaatc gggaacgtct ttagtaacca ccgcaccggc tccgacaaga
      841 gcgctgcgtc cgacggtatg accacatacg atggttgagt ttgcgcctaa cgaagcacct
      901 tcttttacta aggttttgat gtaaaactct gcaccgactt gcggatactt gcagcgcgga
      961 tcaagaatat ttgtaaaaac catcgaaggt ccgcagaaca cataatcttc aagctccacc
     1021 ccttcgtaga tggacacatt attttgtact ttgcaaaaat taccgacttt tacatgattg
     1081 gccacattta cattttgacc aaaaacacat ttttttccga tacgagcgcc ggtttggata
     1141 tgcgaaaaat gccaaacttt agtaccttcg ccgatttcga cgttgtcatc cacgacagcc
     1201 aagggatgaa caaaatattt tttttcagac atgttgcgtg atcctttctt gtgctttttc
     1261 caatatccga agcacttcca gcgcatgttc gccgtccgta cgcggtgttt tgccgtgcag
     1321 gaggcaatca taaaaatgac gatgttcttc acttaatggt tgtgcggcat caaacggaat
     1381 cacctcaaag tctacctcaa atttctcgat ttttccgtta ctttgctgaa agcctttttt
     1441 ataaaatttt aacttatctg ttttgagcga atcttcaaac acgatcattc ccttatcacc
     1501 gatgacaacc agtctttgtt ctttaaaagg gtgaagccaa ctcacaaaaa catgcacatg
     1561 aatgttaccg ggataggtga ggtacgtgat ggtcgaatct tcgacgtttt tttgtacgaa
     1621 cgccgcacct ttagcttcga tcgtttccgg attttttcct atcaaatatt gaactatcga
     1681 aatatcatgc ggtgcaaaac tccaaagact attttcttcg ctgcgaatag cgccgagatt
     1741 gagccgatta ctgtaaatat actgaagttt gccgatatac ccttcatcca tcatctgttt
     1801 caacttcaaa accgccggat gatacaaaag cacatgccct accatgattt ttacatcata
     1861 tttttgagct aaactcaaaa gttcaacagt atgttcagaa attagcgtga ttggtttttc
     1921 gatcagtaca tgtttgccgt tttcgataca tcgtttagca agatcaaaat gggtttcggc
     1981 cggcgttgca atcactacgg cattgatatt cgaatgcgcg atgagcgaat gaatatctga
     2041 tgtaaatgga attttcggtg aaactgtatg cactctttgg atggcgtcgg gtttggcatc
     2101 cgccgttaat aaaagattac ttcctaataa cccatgggcg gtacgcacat gattgagtcc
     2161 ccaatttcca caaccgataa tacctaattt tacttttgta taatccataa tttattttac
     2221 agtaaaatat ataagtataa tggttgatgt gatggccgcc agttgacctg ttatagtcag
     2281 cgcatcttta aatatctgcc aaccatctac cggttctttt tccggaacaa aaatcacatc
     2341 gccgtcttcg atcagtacat catcatcggc atccagccat ttctgcgaag ccgttttgat
     2401 gactttaata tcaccttttt tagctctatc cgtaaaacca ccgcacttgt caatataata
     2461 tcggtaatct ttgccgtgat caaaatctac tatacctgac aataacgcgc cgccaacaac
     2521 ggtcacggtt cgccggatag atgacacatc aatataatca ccgcgcttta aaacaacatc
     2581 atactccgaa ttcactttcc cgtcagcaaa tagtttttta aagtcagttt gtacgacggg
     2641 tttttgttga cgtgttttgg ttttgaagta agacttttca atgtcgttca tctcggacac
     2701 cggtgtcaat ttcaggcgct catattcttg atcgatatcc tcttttgcgg gactgcgata
     2761 aatggaaatg cgatccaatg atacatacgg cgtaaaacca ccggcccttt taatcacatc
     2821 cgaaacggtc gttttatctt cgataatggc ataggatcca ggatacatca cttcgccttg
     2881 gatagctaca tcagcccgat catgatactg cgcgtgtttt cggaaataaa tacgatcatc
     2941 cggtttgaga cgcagattga tcgtggtatc ctccggattt tgatacgctt cacgcaaatc
     3001 gcgcgtaata cgtatcacct ctcggcctgt atccttggcc atacggtaca tgatgaaaat
     3061 ggatgagtca atcccctgca ctaaaccacc ggccaatcga atagcatcga tgatattctc
     3121 attcgccacg tactccatag taccatgact tgctacagca cccaaaatct gataacttgc
     3181 atattgagca ggcacaaata tacggtcacc gtcgttaagt aatggatccg aatccaaatc
     3241 taaatttcgg ccgcgtttta taaaatcaac actctgcgat gtaccgtcct tacgatacag
     3301 aataatattt ctttgcgatg cctttccgtt taaaccgccc gctttcgcaa tggctgtcga
     3361 tacccgttct gttcctgaga ccactaccga actcggatta ttacacatac cccctacggg
     3421 cacgcgaaaa gagcgaagtg aaaccaggtt gatcgtcacc tcggcattgg tatacacagt
     3481 tctgatactt tttcctacac gctcctgcac atcggctaga ttgagatttt taacatctat
     3541 ggcttttact gtaggaacaa tcaaaaaacc ttcgggcgat acaacggttc tgaagttttg
     3601 attgacctgc ccccaaatcg aaactaaaaa ctgatccccg ggaccaacat agtattgttt
     3661 tggatctaca accgtctcaa gaatattatt ggatttttca gggaataaaa catcgtccgg
     3721 gtcaccggaa gagttatcct tttctaattc ctctttgatt ttagatcgat cctgttgcgc
     3781 caatactgtg gtatccgtca tcgccgaaag aatcagagct gaaatcattg ttaagaataa
     3841 atgctttttc atacaagatc cttcagtttt tcggcaatac gctccgccgc cataccatcc
     3901 catagagccg gaatatttcc ttttttgtag ctacggttca atacggcttc catacacgat
     3961 ttcaacgcat cccgatcttg tcctaccaac gtattagtac cgacatccaa agtaaccggc
     4021 ctttcggtat tggcgcgcat ggtaagacat gg
//
LOCUS       DAOQNQ010000290         4027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_10178_length_4027_cov_5.459215, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000290 DAOQNQ010000000
VERSION     DAOQNQ010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10178_length_4027_cov_5.459215"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="PK754_13540"
     CDS             <1..713
                     /locus_tag="PK754_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10573.1"
                     /translation="EQFYTESGSLHRIIWAGTNRALDNSEESGLSVTADSGATWRVVL
                     RGPFVHNITFDGKTVYVATSDGLFRSSNLGQSWERFITIIDQTSGDRIFTSEFTTIGV
                     TPKSGTHTIFAGTYDGLGISSDDGVTWTIPRAYAKPGQKGNPSSYAYPNPFSPKYGIT
                     RFQFDKDNLSSDDRVTLSIYDFSMAPVVTVVKNVPLEQTIFWNGTNQKGQRVANGTYI
                     YIIEAGKKKFHGKVTVRK"
     gene            716..1684
                     /locus_tag="PK754_13545"
     CDS             716..1684
                     /locus_tag="PK754_13545"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNB10574.1"
                     /translation="MPRRYLYIPTSFLIWMQWMLFIITTAATSFAGDNGGRAGSFLRL
                     GLGPRARAMGDAYTALADNVYAPYYNPAGLPLIAQPEVAVSTGILSFDRQFTSAGFAK
                     FLPPKAGFGINILRSGFGESDVRDADGNATGEHIEDTQYTVMFGFALRFGEKFSAGIA
                     PQWIHTQIYDVTASSVGLNIGALYQYSPVWSFGVSLKNLLQKLQYSRNATGLGDETTV
                     DKLPATLRLGVAHHHSLNENFSMVASYDLEKTSDYTLAHHMGAEIGWQNKFFLRTGLD
                     QYDWTAGLGIPFDTFGKKLIVDYTVILDGRKGISTVSHDMGLYVRF"
     gene            complement(1776..>4027)
                     /locus_tag="PK754_13550"
     CDS             complement(1776..>4027)
                     /locus_tag="PK754_13550"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNB10575.1"
                     /translation="SGQIGKTIAYDILRDNQIITLDVTLAKFGIPFFLLVIYGAGLVY
                     ILVGSFLVYKRADTTAAVLLGMAFFLIGMMLSLLGERGSLSTTPFGVFVTNVRWWSLV
                     LGMAMLMHSRLYFPFSVQELIQKKWVAIVPYGLSAITFIGIVVYKQALAAFVSGIAMM
                     VYYIFIRLRYKSYFSSSYRSVSRLSGWIAFACAVLGYVLTFFSDGNSGRGYLFGYAGF
                     FFALIPLAHLYTIARYHLMELNFRVRRNVQYVIASLVWNLTVVYGAVQLMVIAVTSNM
                     DLPNIRFTESSVEITDVPVSADERIQILKVVLILLAVAMTFVWVRLRSWGQRWIDYKF
                     HRSHHDYRRAAAELAEVMATNLNMNDLARGMVEKLASLMHLKMAGVVFYRDEKSLCCS
                     EVYGYESGAWQALCSKSDQPLMAAVRANTSTATINVDNLPGPLRNELRQCGFRFVIPI
                     RSKEHTVGAIIVGERMSESPFDHDDLMFLTTVAKQASVAIENAFLYENLAEQDRLKHE
                     LNIARRIQLESLPQKTPNVRGLEIAGRSVPALEVGGDYFDYLNGKPDKFTVIVGDVSG
                     KGTSAALYMSKVQGIIHSLNSFDLTPRELFIRSNRILCRDLEKKSFVTSIGATFLLEE
                     KKIRLARAGHLPLYCYNASQRKVELITPLGVGLGITDDGTFEENLEEKEFAYAPGDIF
                     VFVTDGVIEAHNIHRHEYGEERLLKLIDEKPDSSAEMLVHRIIDDVHTFTGEAMQHDD
                     ETVVVIKAL"
BASE COUNT         1119 a         1026 c          870 g         1012 t
ORIGIN      
        1 ctgaacaatt ttatactgaa tccggttcgc tccatcgcat tatttgggca ggaactaatc
       61 gtgcccttga taactcagaa gaatccggtt tgagcgtaac cgccgatagc ggcgcaactt
      121 ggcgtgtcgt tcttcgtgga ccttttgttc acaatatcac cttcgacgga aaaacagtgt
      181 acgttgctac gtcggacggt ctttttcgct cgtcgaacct cggccaaagt tgggaacgtt
      241 ttattaccat tatagatcaa acaagcggcg atcgtatttt tacgagcgaa tttacaacca
      301 tcggtgtcac tcctaaatcc ggaacgcata ctatctttgc aggaacctat gacggtttgg
      361 gcatttcttc tgatgatggt gtgacttgga ccataccgcg cgcgtatgcc aagcccggac
      421 aaaaaggtaa tccatcgtcg tacgcctatc caaacccttt ctctccgaag tatggcatca
      481 cacgattcca atttgacaaa gacaaccttt catccgatga tcgcgtaacg ttatccatct
      541 atgatttttc tatggcgccg gtcgtcactg ttgtaaaaaa tgtaccgctt gagcaaacaa
      601 ttttctggaa cggcacaaac caaaaaggac aacgcgttgc caatggaacg tacatttata
      661 ttatcgaagc cgggaaaaag aagttccacg gtaaagtaac ggtgagaaaa tgaacatgcc
      721 gcgacgctat ttatatattc ccacaagttt tttgatctgg atgcaatgga tgcttttcat
      781 cattaccact gccgccactt catttgccgg agataatggc ggacgcgcag ggtctttcct
      841 tcgcctggga ttaggtccgc gtgcacgcgc tatgggtgat gcgtacacgg cattggctga
      901 taatgtgtac gccccatact acaatcccgc agggttgcct ctgatcgctc aaccggaggt
      961 tgccgtttca acgggtattt tgagttttga tcgccaattc acttctgcag gattcgccaa
     1021 gtttcttccg cccaaagccg gatttgggat taacatactg cgcagcggtt tcggtgaaag
     1081 cgatgtacgc gatgccgacg gcaacgctac cggcgaacat atcgaggata cacagtacac
     1141 cgtgatgttc gggttcgcat tacgtttcgg tgaaaaattt tcagccggta tcgcaccaca
     1201 atggattcac acccagattt acgatgtgac cgcttcgtcc gtcggattaa atatcggagc
     1261 tttgtatcaa tattctccgg tttggtcgtt cggtgtttca ctcaaaaatc tcttacaaaa
     1321 gcttcaatac tcccgcaatg caaccgggct cggcgatgaa accacggtgg acaagcttcc
     1381 ggcaacatta cgactcggcg tcgcacatca tcattcgctc aacgaaaatt tctcgatggt
     1441 cgcatcctat gatctggaaa aaacatccga ttacacattg gcgcatcaca tgggagcaga
     1501 gatcggctgg caaaacaaat ttttcttacg tacaggcttg gatcaatacg attggaccgc
     1561 ggggttggga atcccatttg atacatttgg aaaaaaactc atcgtggatt acactgttat
     1621 attggacggg cgcaaaggca ttagcaccgt atcgcacgac atggggctct acgtcagatt
     1681 ttgatactct acgtttttcc ggaactattt cgattctgaa aattaaaatc aacacgtaag
     1741 cggtgagaaa cctacgtagc gtacgaactg attttttata atgcttttat cacaacgacc
     1801 gtctcgtcat cgtgctgcat ggcttcaccg gtaaatgtat gcacatcatc aatgatccga
     1861 tgcaccaaca tctccgctga ggaatccggt ttttcatcaa tcagtttcaa taaccgctct
     1921 tcaccgtatt cgtgacgatg aatattgtgt gcttcgatca cgccatccgt tacaaaaaca
     1981 aagatatccc ccggtgcata tgcgaattct ttctcctcta gattctcctc aaatgttccg
     2041 tcatcggtta tgcccaaacc tacgccaagc ggcgtgataa gctctacctt ccgttgggac
     2101 gcattgtaac aatacaacgg taagtgccct gcacgtgcga ggcgaatttt tttctcttcc
     2161 aatagaaacg ttgcaccgat cgaggtgaca aatgattttt tctccaaatc acggcacaaa
     2221 atacgattgg aacgaataaa caattcgcgc ggtgtcagat caaagctgtt gagcgaatga
     2281 ataataccct gcactttgga catatataac gccgccgacg tgcctttgcc gctcacatcg
     2341 cctacgataa cagtaaattt atccggttta ccgttgagat aatcgaaata atcaccgccc
     2401 acttcgagcg ccggtaccga acgtcctgcg atttccaagc ctctcacgtt cggtgttttt
     2461 tgaggcaatg actccaactg tatgcgccga gcgatattca gctcatgctt caaacgatct
     2521 tgctcggcaa gattctcgta cagaaaggca ttctcgatcg caaccgaagc ttgtttggcg
     2581 accgtcgtta aaaacataag atcatcatga tcaaacggcg attccgacat gcgttccccg
     2641 acgatgatcg cgccgacggt gtgttctttt gagcgaatcg gtataacaaa acgaaaaccg
     2701 cattggcgca attcgttgcg cagcggtccc ggtaaattat ccacattgat cgtagctgtt
     2761 gacgtattgg cgcgcacagc cgccataaga ggttgatcgg acttcgagca taacgcctgc
     2821 cacgcgccgg attcataccc atatacttca ctgcaacaca acgatttctc atcccgataa
     2881 aaaacgacgc cggccatttt gagatgcata agcgaagcta atttttcgac cattccgcgc
     2941 gccaaatcat tcatattaag attggtcgcc atcacctccg caagctctgc cgccgcacgc
     3001 cggtagtcgt gatgggatcg gtgaaattta taatcgatcc agcgttggcc ccatgatcgc
     3061 agacgtaccc aaacaaacgt catcgccacg gccaacaata taagaacgac tttaagaatt
     3121 tgtatgcgtt catccgcact tacaggtaca tccgtaattt cgacagaaga ttccgtaaaa
     3181 cggatgttag gcaaatccat attcgaagtc accgcgatga ccatcaattg cacagcgcca
     3241 tacaccacgg tcaaattcca caccagcgat gcgatcacat attgtacatt gcgacgcaca
     3301 cggaagttta gctccatcaa gtggtatcgt gcgatcgtgt acagatgcgc gagcgggatc
     3361 agcgcaaaga agaatccggc ataaccaaaa aggtagccac gtcctgaatt accgtcactg
     3421 aaaaacgtca atacgtaacc caaaacagcg catgcgaaag caatccaacc gctcagacgt
     3481 gaaaccgaac gatatgatga tgaaaaatat gacttgtaac gcaatcggat aaatatatag
     3541 tacaccatca tcgcaatgcc gcttacaaac gctgcaagcg cttgtttgta aactacaatt
     3601 ccgataaacg ttatcgccga taggccatag ggtacgatgg ctacccattt tttttgaatt
     3661 aattcctgta cggaaaacgg aaaatataat cggctgtgca tcagcatggc cataccgagt
     3721 acgagactcc accatcgcac attggtcaca aataccccga atggcgtggt actcaaggat
     3781 ccgcgctcac ctaaaagcga caacatcatt ccgataagaa aaaacgccat tcccaaaagc
     3841 acggccgctg tggtatccgc gcgtttgtat accaaaaatg atcctactaa tatatacaca
     3901 agaccggcgc cataaatcac caacagaaaa aacggtatgc caaacttggc cagcgtaaca
     3961 tccaaggtaa tgatttggtt gtcgcgcaaa atatcgtaag caatggtctt accgatttgt
     4021 ccggatg
//
LOCUS       DAOQNQ010000291         4008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_10255_length_4008_cov_6.047812, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000291 DAOQNQ010000000
VERSION     DAOQNQ010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10255_length_4008_cov_6.047812"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..921)
                     /gene="fsa"
                     /locus_tag="PK754_13555"
     CDS             complement(268..921)
                     /gene="fsa"
                     /locus_tag="PK754_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HNB10576.1"
                     /translation="MKFFIDTANLDEIREAASLGILDGVTTNPSLVAREKNVNFHDLL
                     KEICKVVDGPISAEVVSTDLDGMLKEGYELAKIHSNITVKLPLIKSGLQACKRFTSDG
                     IKTNVTLCFSPNQALMAAKAGATFISPFVGRLDDISTNGMQLIEEIVNMYINYGYETQ
                     VLVASVRHPMHVVEAAQIGAHVCTMPYKVIDQLIAHPLTDLGLKKFLDDWAKVKAGK"
     gene            1136..3142
                     /gene="uvrB"
                     /locus_tag="PK754_13560"
     CDS             1136..3142
                     /gene="uvrB"
                     /locus_tag="PK754_13560"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNB10577.1"
                     /translation="MEFKLKSDYALQGDQKEAVAHLVRGIRDGQQHQTLLGVTGSGKT
                     FTMANVIAQIGKPTLVLSHNKTLAAQLYGELKQFFPDNAVEYFISYYDYYQPEAYVAS
                     TDTYIAKDMSINDEIEKLRLRATSSLLERQDVIIVASVSCIYGIGSPQDYKDMFVFTE
                     KGQTVDRNKMLARLVDMQYNRNDIAFERGTFRVRGDVIDIYPAYEDHYVRIELFGDEI
                     EQIAYLDPVTHKIITKPATAIIFPATHFVTSESKLHQAILRIQKELEERLTFFRSQNR
                     LVEAQRLEQRTKYDIEMMLELGYCSGIENYSRHIDGRGEGDRPACLLDYFPKDFLMFI
                     DESHVTVSQVRAMYNGDRARKTNLVEYGFRLPSALDNRPLKFEEFEKMLSQVVYVSAT
                     PADYELQKSGGEIVEQIIRPTGLLDPMIEVRPVKNQIDDLIHEIRKRTQVGERVLVTT
                     LTKRMAEDLTEYLANLKIRVRYMHSEIDSLERVDILRDLRLAGFDVLVGINLLREGLD
                     LPEVSLVAVLDADKEGFLRSERSLMQTAGRAARNANGTVIFYADKMTDSMKLVIDETG
                     RRRAMQMAHNEKHGITPTTVFKSKEQIIRSTSVADYFTYKEGQEEAQALAASEATIDY
                     QTMLSADDRKAMVSKLKTEMQEAVKKLEFERAASLRDQIIRLEK"
     gene            3234..>4008
                     /locus_tag="PK754_13565"
     CDS             3234..>4008
                     /locus_tag="PK754_13565"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB10578.1"
                     /translation="MKKLTSWFAILLIATVIKSCGGCNERQLLEKAKPYYEKAVELEQ
                     NGQFSSAVAFYDTAIQIFPDYRDAYFQMGTVYERLVLPEKAAASYRAAIRIDADFAAA
                     HNNLGNCYGKLNKLDSALVHLKKAIQLDPQSPSAHYNLGHAALLKGDFSGAETAFTAA
                     LALRPSDPDYLQALAMLYVTQNKLAEALPLYERAALQRLAQPELRYRMALVYRDQKMY
                     PSALTELEAYLKTRADPQEQTMIRRLIGEIKVEQSREKLA"
BASE COUNT         1154 a          952 c          859 g         1043 t
ORIGIN      
        1 ttaaaaaaag gattcccgta ggtaaattct tgtatctgta cgacgttgat accgacgacg
       61 gcaggtaacg cctttttgac ggatcgggta atcgcatttt gacgcgacga cgtaatgtca
      121 tcttgcgcaa caagagagaa acatacgaag aaaaatatta aaccccagcg cttcatccgg
      181 aacctcctga aatgttgtac ttcattatat gcaaccttgc ttacgttggg taaatccaaa
      241 aggttccggc gcgtaccgat tcaatcatta ttttccggct tttactttgg cccaatcgtc
      301 aagaaacttt ttcaaaccga gatcggtcaa cggatgcgca atcaactgat caatcacttt
      361 ataaggcatt gtacaaacat gcgcaccgat ctgagccgct tcgaccacgt gcatcggatg
      421 acgtacgctg gccacgagga cttgcgtctc ataaccatag ttgatataca tatttacgat
      481 ttcttcgata agttgcatgc cgttggtgct gatatcgtct agacgaccga caaaagggct
      541 gataaatgtg gcacccgctt tcgcagccat caaagcctga ttgggcgaaa agcatagcgt
      601 cacattcgtc tttataccgt ccgacgtaaa acgtttgcaa gcctgtaatc cggatttgat
      661 caatggtaat ttgaccgtga tgttggagtg aattttggct aattcgtaac cttctttgag
      721 catgccgtcc agatcagtac tgaccacttc cgccgatata ggtccgtcaa cgactttaca
      781 aatttctttc agcaaatcat gaaaattgac gtttttttcg cgcgctacca gagatggatt
      841 ggttgtcaca ccatccaaaa tacccaacga tgcggcttcg cgaatttcat ctaaattggc
      901 ggtatcaata aaaaacttca tacaacgacc tcctgcggtt aataaacggc cttaactctt
      961 cggttaattt tagcagaacg ttctcaaaat agccctttga ttttatccaa tcaaggctat
     1021 ttctgcatac aaaatttatg caaagcacaa gcgcattatt gatacatttg ttttttcatt
     1081 gttatttttt atatactgac ataaggttgt cacaacgatc gagtatattt gatgcatgga
     1141 atttaaactc aaatccgatt acgccctaca aggcgatcaa aaagaagccg tagcccatct
     1201 tgtgcggggc atccgagacg gacagcaaca ccaaactcta ttgggtgtta ccggttcagg
     1261 taaaacattt accatggcta atgtcattgc tcagatcggc aaacctaccc tcgtactctc
     1321 tcataataaa accctggcgg cgcagcttta cggtgaactc aaacaatttt ttccggacaa
     1381 tgctgtcgaa tattttatca gttattacga ttattaccaa cctgaagcct acgtcgcgtc
     1441 aacggacacc tacattgcta aagatatgtc catcaatgac gaaatcgaaa aattacgcct
     1501 tcgcgctacc agttcgttat tggaacgtca ggatgtcata attgtcgcat cggtaagttg
     1561 catttacggt atcggatcac cgcaagatta caaagatatg tttgttttta cagaaaaagg
     1621 tcaaaccgtt gatcgcaata aaatgctcgc ccgtttggta gatatgcaat ataaccgcaa
     1681 tgatattgct tttgaacgcg gcacatttcg tgtgcgcggt gatgtgattg atatctatcc
     1741 ggcctacgaa gatcactatg tccgtatcga gttattcggc gatgagatcg aacaaatcgc
     1801 ttatcttgat ccggtaacac ataaaattat cacaaaaccg gcaacggcca ttatttttcc
     1861 ggcgacacac tttgtcacct cggaaagcaa attgcatcag gccattctgc gcatacaaaa
     1921 agaactcgaa gagcgtttga cttttttccg gagtcaaaat cgcctggtcg aagcccaacg
     1981 actggagcaa cgcaccaaat acgatatcga gatgatgctg gagttaggct attgctcggg
     2041 tatcgaaaac tactcgcgcc acatcgacgg ccgcggtgaa ggtgacagac cggcctgttt
     2101 actggactac ttccccaaag attttctgat gttcatagac gaatcccacg taacggtatc
     2161 gcaggtgcgc gctatgtata acggcgatcg cgcacgaaaa acgaatctag tcgaatatgg
     2221 atttcgtctg ccttccgctt tggataatcg cccgctcaaa tttgaagaat ttgaaaaaat
     2281 gctgagtcag gtcgtgtatg tcagcgccac ccccgccgac tatgaattgc aaaaatccgg
     2341 cggagaaatc gttgaacaaa ttattcgtcc gaccggtttg cttgacccta tgatcgaagt
     2401 gcgtcccgta aaaaatcaga ttgatgatct gatccacgaa atccgcaaac gaactcaggt
     2461 cggtgaacgc gtgttggtca ccaccttaac gaaacgtatg gcggaagacc tcacggaata
     2521 tttagccaac ctcaaaatac gggttcgcta tatgcactcc gaaattgatt ctttggaacg
     2581 agtggatatc ctgcgcgatt tgcggttggc gggttttgac gtattggtag gtatcaatct
     2641 tctaagagaa ggcttggatc ttccggaagt ttccctcgtt gccgtacttg atgccgacaa
     2701 agaaggtttt ttgagaagcg aacgctcgct gatgcaaaca gccgggcgcg ctgcgcgtaa
     2761 tgccaacgga accgtgattt tttatgccga taaaatgacc gactcaatga aattagtcat
     2821 tgatgaaacc ggtcgtcgcc gtgccatgca aatggcacat aacgaaaaac acggtataac
     2881 cccgaccacg gtttttaaat caaaggagca aattatccga tccacctcgg ttgccgatta
     2941 ttttacctac aaagaaggcc aggaggaagc tcaagcttta gcggcctccg aagcgacgat
     3001 agactatcag accatgttga gtgccgatga ccgcaaagcc atggtaagca aactcaaaac
     3061 ggaaatgcag gaagccgtaa aaaaattgga atttgaacgt gccgccagcc tccgcgatca
     3121 aatcattcgc ctcgaaaaat aaaaccaaaa cagccgtata ttataaatcc tccgcataat
     3181 tgtttttact gtttctcgtt ggcattttag ccgcgttcaa ttatattgcc accatgaaaa
     3241 aattgacctc atggtttgca atacttttaa ttgcgaccgt tatcaagtcc tgcggcggtt
     3301 gtaatgagcg acagcttcta gaaaaagcaa aaccttatta tgaaaaagcc gtagaacttg
     3361 agcaaaacgg acagttttcg tcagccgtgg ccttttacga tacggcgatt cagatttttc
     3421 cggattaccg cgatgcttat tttcagatgg gcacagttta tgaacgcctc gtattgccgg
     3481 aaaaggccgc cgcatcatac cgcgccgcaa tccgcattga cgcggacttt gcagccgcac
     3541 ataacaacct cggcaattgc tacggaaagc tcaacaaatt ggacagcgct ttagtacact
     3601 tgaaaaaagc tatccagttg gatcctcaat ccccttcggc tcattacaat ttaggtcacg
     3661 ccgcattgct caaaggcgat ttctccggag cagaaacagc ttttactgca gccttagcat
     3721 tacgtccatc cgaccctgat tatttgcagg cattagcaat gctgtatgtt acacaaaaca
     3781 aattggctga agcactgcct ctttatgagc gcgctgcctt gcaacgtctg gcacaaccgg
     3841 aattgagata ccgtatggcg ttggtatatc gcgatcagaa aatgtaccct tcggcattaa
     3901 ccgaacttga agcctacctg aaaacgcgag ccgaccccca ggaacaaacg atgataagac
     3961 gtctgatcgg cgaaataaaa gtcgaacaaa gtcgcgaaaa actggctc
//
LOCUS       DAOQNQ010000292         3998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_10307_length_3998_cov_6.134669, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000292 DAOQNQ010000000
VERSION     DAOQNQ010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10307_length_3998_cov_6.134669"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="PK754_13570"
     CDS             complement(<1..1027)
                     /locus_tag="PK754_13570"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNB10579.1"
                     /translation="MKMFSNEPVKLKKADRPLILAIDIGTSSTRAVVYDAQGRSVKKL
                     VHQVGYMMQVTPDGGVFADPKFIVDATAQCIDAIMEAMGKDANDIKAVGFDTFWHNMM
                     GIDMRGKPTTPLINWADTRPRIVIDDLRKRIDPEEAHRLTGCVLHPSYLPAKLLWMHK
                     YNKEDFRRTTTWMSIGEYLYYVFFGKVICGISMASGTGLFNQNTCNWDEKILSVLPIK
                     PGQLGELGDLDTPLRGLKSEYARRWPALAQIPWLPAVGDGASSNVGTGAVKKDQLCIV
                     MGTSGALRVMWEAKDVTIPSELWVYRVDRKRFLMGGALSDGGNLRKWMLSRLAIEGGK
                     KVWMQPST"
     gene            complement(1156..1707)
                     /locus_tag="PK754_13575"
     CDS             complement(1156..1707)
                     /locus_tag="PK754_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10580.1"
                     /translation="MKTIRMISVFLLAGIGGCGDDTTALNDPWEAFAEGRYQDAHSLF
                     LDLIETKPSEAYAGLGWTTLKMNADSLNAANRYFSLSSNDSLVYAFAGWALASWASEN
                     YMDCIKYGDFVLRKNPEFVFDYLQTLTQKQIALAQAYSYFHLQNLSACISKIRIIEPS
                     YTPVNNLDRNEIQLKLDQLRQTQ"
     gene            complement(1731..>3998)
                     /locus_tag="PK754_13580"
     CDS             complement(1731..>3998)
                     /locus_tag="PK754_13580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB10581.1"
                     /translation="IGADGVTLLTNKLYGILFNAHSHHNMIGDGTIGGRNVISGNNDG
                     SARAMLVSSRHNRILGNYIGVLPDGMTLAFNNDGIWLENDSNHIGDGTAGGRNIISGN
                     SNGIVITSKARNNKILGNYIGIASDGAASVPNVRGIFSLSSTHLQIGDGTSGGRNIIS
                     GNTQVGIRLTSTDSALVFGNYIGVGSDALTPMANSIGLQLDNASFSNRIADNIIANSS
                     QYGIQTSDATTLKNTFFKNRIYKTTIQPISQVSGAQESVWPPAIFANGGNVYTGLASK
                     GALVQFFADSSDEGLMFLDTVFADPSTGKWSKTLIVPSGMNLTAMQDSAGNSSVFSTA
                     YEPIRNVAPSIPVLLTPQSNALTNNLRPTFTWHKSIDGDDDDVFYQILVSKNSDLSDA
                     QLTVSNITDTTFTSGTDLDSTFGYYWRIIAVDNKGGAAQSAVRFFKTDAKKPKLTASL
                     HVMKAIERYVQVYIASDETLVSRTVSFSFSGNTIQKTPDLIADGLYQVNYEIATSEGL
                     LSVIIQGGDSAGNVQSSSLSYQVTSLNKKSGVSISHQMLTLDVPPNATSGDGLLMVTS
                     FKSDQNIHKVNSVDLVPVSDTYELISAVSIMRPWNLTIRFNELESSEWDERKVSLYAQ
                     RDGQWVYVGGEGRHGSVTAKISGASRYRLFYNPSHVILPVKIQLDQNFPNPFNPTTSI
                     RFGLPQDGHIRVTVYNILGQFIAELLNENRTAGYHNVIWNGRNATNQQVSSGIYLYRL
                     ETSQGVITRKMMLLK"
BASE COUNT         1101 a          944 c          834 g         1119 t
ORIGIN      
        1 gagttgaggg ctgcatccac actttttttc cgccttcgat tgctaaacgg cttagcatcc
       61 acttgcgtaa attaccgcca tcactcaaag cacctcccat aagaaatctt ttgcgatcaa
      121 cccggtacac ccacaattcg gatgggatag ttacatcctt cgcctcccac ataacccgta
      181 gcgcgccgga ggtacccatt acaatacaaa gttgatcttt ttttacagca ccggtcccta
      241 cgttggagct ggcgccatca ccaacagccg gaagccatgg aatttgagcc aaagccggcc
      301 aacgacgcgc atactctgat ttgagcccgc gcaaaggcgt atccaaatcg ccaagttcac
      361 cgagttgacc cggcttgatc ggtagtacgc tcaatatttt ctcgtcccaa ttgcaggtgt
      421 tttggttaaa aagccccgta cctgatgcca tagaaatgcc gcaaatcact ttcccaaaaa
      481 aaacgtagta cagatattcg ccgatggaca tccatgtggt cgttcggcgg aaatcctctt
      541 tattatattt atgcatccac aataacttcg ccggtaaata actggggtga agcacgcatc
      601 cggtaaggcg atgtgcttct tcaggatcaa tgcgtttacg aaggtcgtca ataactatgc
      661 gtggtctggt atccgcccaa ttgatgagcg gtgtcgtcgg tttaccacgc atatcaatac
      721 ccatcatatt atgccaaaat gtatcaaacc ctactgcttt tatatcattg gcatcttttc
      781 ccatcgcctc cataatcgca tcaatacatt gcgccgtagc atccacgata aacttaggat
      841 cggcaaaaac accgccatct ggcgttacct gcatcatata ccctacttga tgaactaatt
      901 ttttgactga acgtccttgt gcatcataca ccactgcacg cgttgaggaa gtcccgatat
      961 caatcgctaa aattaacggg cgatcagctt ttttgagttt aacgggttcg ttagaaaaca
     1021 ttttcatacg cgtctccggt gatggtacaa ttaggataca taaggcaggc gcctcatttt
     1081 agccatttcc atgaatgcct tcaagcagaa aaaaatcccg catcgttagt acgggatttt
     1141 tatgtcttaa tttatttatt gagtttgtcg caactggtca agtttgagct gaatttcatt
     1201 gcgatctaga ttattgaccg gcgtgtacga aggttctatg atacgtatct tagaaatgca
     1261 cgccgataaa ttttgaagat ggaaatagct atacgcttgc gccaaagcaa tctgcttttg
     1321 agtcagcgtt tgtaaatagt caaaaacaaa ttcagggttt tttcgcaata caaagtcccc
     1381 atacttaatg cagtccatat agttttctga agcccatgac gcgagggccc aaccggcgaa
     1441 tgcataaaca agtgaatcat ttgatgatag actgaaatac ctgtttgccg cgttcaatga
     1501 atcagcgttc attttgagag ttgtccaacc caatccggca taggcttccg acggtttcgt
     1561 ttctattaaa tccaagaaaa gagaatgcgc atcctgataa cgtccttctg caaaagcctc
     1621 ccacggatcg ttgagagccg tcgtatcgtc cccgcaccca cctattcctg ccaaaagaaa
     1681 tactgaaatc attcttatag ttttcatact attttccttc gttattgatg ttacttcaaa
     1741 agcatcattt ttctcgtaat cacgccttgc gaagtttcta agcgatataa atatatcccg
     1801 ctagaaactt gctgattggt tgcattacgg ccattccaaa tgacgttatg atagccagct
     1861 gttcgatttt cattgagcaa ctccgctatg aattggccga gtatgttgta caccgtgaca
     1921 cgaatgtgcc catcctgcgg taacccaaat cgaatagatg tcgtcggatt aaaaggattt
     1981 gggaaattct gatctaactg aatttttaca ggtaagatca catgtgaagg attataaaaa
     2041 agccgatatc gggaagcgcc agagatcttt gctgtaaccg agccatgacg tccttcacct
     2101 ccgacataaa cccactgtcc gtccctctgg gcgtagagag aaaccttgcg ttcatcccat
     2161 tcgctacttt ccagttcatt aaaacgaata gtcaaattcc atggacgcat tatagataca
     2221 gcggagatta attcgtacgt atcactaacc ggaactaagt ccacagaatt gactttgtga
     2281 atattttgat cggatttaaa tgacgttacc ataagaagtc cgtcacccga ggttgcattg
     2341 ggtggaacat ccagagtgag catttgatgt gagatactta cgccggattt tttattcaat
     2401 gatgtaactt gataactaag cgacgacgat tgcacattac cagctgaatc cccaccttgt
     2461 ataataacag ataacaaacc ttccgatgtt gcaatttcgt aattgacctg atacaatccg
     2521 tcggctatga ggtccggtgt tttttgtatt gtatttccac taaaggaaaa acttactgtt
     2581 cgggatacca gcgtttcatc actagcaata taaacttgca catagcgttc gatcgctttc
     2641 atgacgtgaa gactggccgt tagtttgggt tttttggcat cggttttaaa aaaacggact
     2701 gcactttgcg cagcgcctcc cttattatca accgctatta tacgccaata ataaccgaat
     2761 gtagagtcca gatccgttcc cgaagtaaat gtcgtgtccg tgatatttga aacagttaac
     2821 tgcgcatctg ataaatcgga attctttgat accaaaattt gataaaacac atcgtcatcg
     2881 tccccatcaa ttgacttatg ccacgtaaat gttggacgaa ggttattagt caatgcattc
     2941 gattgcggtg ttaataacac aggaatagag ggcgcaacat tgcgaatcgg ttcgtaggcc
     3001 gtcgaaaaga ccgaactatt gccggcgctg tcttgcatgg ctgtaagatt cataccggat
     3061 ggaacgatta gtgtcttgga ccacttaccc gtggaaggat cagcaaatac cgtatcgaga
     3121 aacatcagcc cctcgtcgga tgaatcagcg aagaattgca caagggcgcc cttagatgcc
     3181 aatcccgtat aaacattacc tccattggca aatatagccg gaggccacac gctttcttga
     3241 gcgccgctga cttggcttat cggctgaatt gttgttttat aaattctatt tttaaaaaac
     3301 gtgtttttta atgtcgttgc atccgacgtt tgaataccat actgcgagct atttgcaatg
     3361 atattatcgg cgatacgatt tgaaaatgaa gcgttgtcaa gttgcaggcc gatactgtta
     3421 gccatcggtg tgagagcatc tgagccgaca ccgatatagt ttccaaatac aagggcgcta
     3481 tccgtcgaag tcagacgaat gccgacttgc gtgttgccgg agattatgtt gcgcccgccc
     3541 gatgtgccat cgccgatttg cagatgtgtt gatgataagg aaaaaatgcc ccgcacattg
     3601 ggcaccgatg ccgcgccatc agacgcaata ccgatataat ttccaaggat tttattgttt
     3661 cgggctttac ttgtgatcac gatgccgttt gaattaccgg aaatgatatt gcgtcctccc
     3721 gcagtaccgt caccgatatg gttagaatcg ttttccagcc aaatgccgtc gttattgaag
     3781 gcaagcgtca tcccatccgg caaaacaccg atatagtttc ctaaaatccg attatggcgc
     3841 gagctgacca acatggctcg tgcagaccca tcattattac cggagattac attgcgtcct
     3901 ccgatcgtac cgtcgccgat catgttatga tgggaatgtg cattgaacaa aatgccatat
     3961 agtttattgg taagcaatgt caccccatcc gcaccgat
//
LOCUS       DAOQNQ010000293         3997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_10312_length_3997_cov_4.963217, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000293 DAOQNQ010000000
VERSION     DAOQNQ010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10312_length_3997_cov_4.963217"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PK754_13585"
     CDS             complement(<1..276)
                     /locus_tag="PK754_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB10582.1"
                     /translation="MIKAKNLNKMYTTDEVETTALNNVNFEINDSEFVAIMGPSGCGK
                     STLLNIIGLLDNPTSGEYHFVGHEVSRYSERQRSNLRKANIGFVFQSF"
     gene            complement(304..696)
                     /locus_tag="PK754_13590"
     CDS             complement(304..696)
                     /locus_tag="PK754_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNB10583.1"
                     /translation="MNENGMLNLGHKNLDVWKLSIELVTKIYAITENFPKTEMYGITN
                     QLRRAAVSVSSNIAEGAARRSQLERRRYYEIARSSLVEIDTQLEIALRLKFCIRENLH
                     SISDLLNHVFASLSKLVKSTIHKTLTKN"
     gene            complement(775..2028)
                     /locus_tag="PK754_13595"
     CDS             complement(775..2028)
                     /locus_tag="PK754_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNB10584.1"
                     /translation="MDRKIEKKTWTPKRIAIIGGGALFVFFIAYQFLFGDNSTKLNVK
                     SDKITIAKVTKGPFQEFIPVTGNVMPIKTIYLDAMEGGRVDKLFVEEGAMLKQGEEIL
                     KLTNTNLQMDVMFREAQLFEQINNLRNTRLSYEQTRLRNKSDLINLDYEIQKLKRTYD
                     IQKGLQSQNLTTPQEFETARDEYDYAAKRRDLLLETMRQDSILRAVQMQSLEASIEHM
                     GTNLNTIKENLANLTIRAPIAGQLTSRNAELGESKRSGDRLGQIDVLDGFKVRAGIDE
                     HYIARIQIGQTGEFDLAGKTYNLVIKKVYPEVQNGRFEVDMEFQGDTPKDIRRGQSLQ
                     IRLELGDLQEAVLVPRGGFYQKTGGQWIFVVDPSGDFAVKRNIRLGRQNPQVFEVMDG
                     IFPGEQVITSSYDSYGDVDKLMLNK"
     gene            2459..3175
                     /locus_tag="PK754_13600"
     CDS             2459..3175
                     /locus_tag="PK754_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10585.1"
                     /translation="MFKILTFTTVILSASLLAQDLSENTPSQGVLRKTMYQRSKTQKD
                     SLRVTRDPARLHVQPYPNFGIGMGGSGYWMLHDKFQHVYKDVGEPTTSPLLWLTMELQ
                     LSKELTIHQNTTLGLAEMGLHTVSASLCYRFLHSGTFQPFIGGGAGIYIFDLYKERGD
                     YLASGHGKKWGLQYVAGIDIGPYNRDTGLTLFAQYADVRPFRGRLHDNVESQYDNTPI
                     TVDISGLMIGARLTFYFGGY"
     gene            3177..3590
                     /locus_tag="PK754_13605"
     CDS             3177..3590
                     /locus_tag="PK754_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10586.1"
                     /translation="MKFTSYSIWVAVFILVSDGCISKTMISRRSELKQAVAEQNKLVV
                     QLKNDRLLVLEEVTYSDTAIYAKGYEIKGDRHIPVSESIVLDDVIYAEYNKRSILGGL
                     VGAGIVGVSAIAAVAIAKDPGKFRMNRGYACGADE"
     gene            complement(3566..>3997)
                     /locus_tag="PK754_13610"
     CDS             complement(3566..>3997)
                     /locus_tag="PK754_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10587.1"
                     /translation="QYTHANSIKKTLIIFKPWRFSEYLFPLAKPQREMKKSFEIIFFM
                     DRLFVNEPFTGRYTHANSIKKTLIIFTPWRFSEYLFPRAKPQREMKKSFEIIFLWIAY
                     LSTNHSHAAIPMPIQLQNRYSYPGMFIALHVDIIYSSAPHA"
BASE COUNT         1001 a          856 c          938 g         1202 t
ORIGIN      
        1 aaaactctga aacacaaaac cgatattggc tttgcgcaga ttggaacgtt ggcgctcgga
       61 gtagcgcgat acctcatgcc cgacaaaatg atattcaccg ctggtcggat tgtcgagtaa
      121 gccgatgata ttgagcagcg tggatttacc gcaaccggat ggtcccatga tcgcgacgaa
      181 ttcgctgtcg ttgatttcaa agttgacgtt attcagtgcg gttgtttcca cttcgtccgt
      241 ggtgtacatc ttgtttaggt tcttggcttt gatcatacag gcatctccta aaagtgatat
      301 ggattaattt ttcgttaatg ttttatggat agtgcttttt actaatttag aaagactggc
      361 aaatacatgg tttagtagat cagagataga atgcaaattt tctctgatgc aaaacttcag
      421 gcgtaacgca atttctaatt gcgtatcaat ttctactaaa gaagaacgcg caatctcata
      481 gtagcgacgt cgttcgagtt gagatcgtcg tgcagcgcct tcggcgatat ttgatgaaac
      541 cgacaccgcc gctcgccgaa gttgattggt gataccatac atctctgttt ttggaaaatt
      601 ttctgttatg gcgtaaattt tagttaccaa ttcgatactc agtttccata catctaaatt
      661 tttatgaccc aaattcagca taccgttctc attcattaca aaatctccta atttattatc
      721 gctttccgtc tgccgtttcc cgtgtcccgt ttcccgtctg ccgtctaccg tctgctactt
      781 attcagcatg agtttgtcca catcaccgta gctgtcgtag ctggatgtga tgacttgttc
      841 gccggggaag attccgtcca tgacttcaaa aacttgcggg ttttggcgcc cgaggcgaat
      901 attccgtttt acggcaaagt cgccggacgg atcgacgaca aaaatccatt gcccgccggt
      961 tttctgataa aatccgccgc ggggtacaag tacggcttcc tgcaaatcgc ccaattccag
     1021 acggatctgt aaactttgtc cgcgacgaat gtccttaggt gtgtcgcctt gaaattccat
     1081 atccacttca aaacgtccgt tctggacttc cggataaact tttttgatca cgagattgta
     1141 cgtttttccg gcgagatcaa attcgccggt ctgcccgatc tgaattcgtg cgatgtagtg
     1201 ttcgtcaatg ccggcacgca ctttaaagcc gtccagcaca tcaatttgcc cgaggcgatc
     1261 gccggagcgt ttggattcgc caagttccgc gttgcgtgac gtgagttgcc ctgcgatagg
     1321 tgcgcggatg gtaagattgg cgaggttttc tttgatggta ttgaggttgg ttcccatgtg
     1381 ctcgatactg gcttccagcg attgcatctg cacggcgcgc aagatcgaat cctggcgcat
     1441 ggtttccaat agcagatcgc gccgcttggc ggcgtagtcg tattcgtccc gcgcggtttc
     1501 aaattcttgc ggcgtggtca gattctgcga ttgcaggcct ttctgaatat catacgtgcg
     1561 tttgagtttc tgaatttcat aatccagatt gatcaggtcg cttttgttgc gcaaacgggt
     1621 ttgttcgtaa gataaccgcg tattgcgcag attgttgatt tgttcaaata actgcgcttc
     1681 gcgaaacatg acatccattt gcaaattggt gttcgtcagt ttgagaatct cttcgccttg
     1741 tttgagcatc gcgccttctt cgacgaaaag tttgtccaca cgtccgcctt ccatcgcgtc
     1801 gaggtaaatc gttttgatcg gcatgacgtt gccggttaca ggtatgaatt cctgaaacgg
     1861 gcctttcgtc actttggcga tggtgatttt gtcggatttg acattgagct tcgtggaatt
     1921 gtctccgaag agaaattgat acgcgatgaa aaaaacaaac aatgcgccac cgccgatgat
     1981 cgcaatgcgt ttgggagtcc atgtcttttt ttcgattttc cggtccatgt tacctgtcgt
     2041 gtttcaatgc ggttgataat tgataaacct aactcaatac tatttattgt caattcttat
     2101 gccaatggtt aaataattgt ttttaatgat tttcaattta taaatgagca cacaaatcgt
     2161 tcgtatgcgt gcaggggtgt acgaatccgt acactctata aataggttcc aaattcaggt
     2221 atcggaggtt ttgctaaaat gattcaatcg ttggttcata gaaaaaatca tttcaatttt
     2281 tttgcatcct aagcacacgc cattttttta tggcagtgtg tttttttaaa tcgcgggttt
     2341 cttcgggagc tttttacaac tctataaaaa actgcgctcg cttttcggca cggtgtttga
     2401 catgatcaga tatgttatta aaacgtcgta gtgattcatt cttcgaggga caacgatcat
     2461 gttcaaaata ctaactttca cgaccgtcat tctgagcgca tcacttttgg cgcaggacct
     2521 ttcagaaaac acgccatcgc aaggcgtcct aagaaaaacg atgtaccaac gttcgaaaac
     2581 tcaaaaggat tcgctccgtg tcacgcgtga tcctgcccgt ctgcatgttc agccataccc
     2641 caattttgga atcggcatgg gcggttcggg gtactggatg ttgcatgata aatttcagca
     2701 cgtatataaa gatgtgggcg aaccgacgac gtcaccgctt ttatggctga ccatggaact
     2761 ccaattgtca aaggaattga cgattcatca aaatacgaca ctgggattag ccgaaatggg
     2821 attgcacacc gtatccgctt cgttgtgtta tcgttttctt cattcaggta cattccaacc
     2881 ttttatcggc ggcggcgcag gtatttatat ttttgatttg tataaagagc gaggtgatta
     2941 cctcgcgtcc ggtcacggaa aaaagtgggg cttgcaatat gtcgctggta ttgacatcgg
     3001 gccgtataat cgcgatacgg gacttacgtt atttgcacaa tacgcagacg ttcggccatt
     3061 tcgcggtcga ctccatgata acgtcgaatc acaatatgac aatacgccga tcactgtaga
     3121 tatcagcggt ctgatgatcg gtgcccgatt aacattttat tttggaggat attgatatga
     3181 aattcacatc gtactcgatc tgggtcgcgg tattcatact cgtctcggac ggttgcatca
     3241 gcaagaccat gatttcccgc cgttctgaac tgaaacaagc ggtggctgaa caaaataaat
     3301 tggtcgtaca actaaaaaac gaccgtttgt tggttttgga ggaggtgacg tattccgata
     3361 cggcgatata tgccaaagga tatgaaatca aaggcgacag gcatattcct gtcagtgaat
     3421 ccatcgtact cgatgatgtg atctatgcgg agtacaataa gcgcagtatc ctgggaggcc
     3481 ttgtcggtgc gggtattgta ggcgtttcag cgatcgccgc cgttgccatc gccaaagatc
     3541 ccggaaaatt caggatgaac cgcggttatg cgtgcggtgc agatgagtaa ataatgtcga
     3601 cgtgaagcgc gataaacatc ccgggatagg aataacgatt ttgtaattga attggcatgg
     3661 gtatagcggc atgtgaatgg ttcgttgaca aataggcgat ccataaaaaa attatctcaa
     3721 aagacttttt catttctctt tgcggctttg cgcgagggaa taggtactcg ctaaagcgcc
     3781 aaggcgtaaa aattatcaaa gtttttttaa ttgaattggc atgggtatag cgacctgtga
     3841 atggttcgtt gacaaatagg cgatccataa aaaaaattat ctcaaaagac tttttcattt
     3901 ctctttgcgg ctttgcgaga gggaataggt actcgctaaa gcgccaaggt ttaaaaatta
     3961 tcaaagtttt ttttattgaa ttggcatggg tatattg
//
LOCUS       DAOQNQ010000294         3956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_10500_length_3956_cov_5.787747, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000294 DAOQNQ010000000
VERSION     DAOQNQ010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10500_length_3956_cov_5.787747"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..107
                     /locus_tag="PK754_13615"
     CDS             <1..107
                     /locus_tag="PK754_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HNB10588.1"
                     /translation="LDPSSEIRWVLANKKVFRLKEDLGTEPKFWYFMD"
     gene            111..1406
                     /gene="nrfD"
                     /locus_tag="PK754_13620"
     CDS             111..1406
                     /gene="nrfD"
                     /locus_tag="PK754_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNB10589.1"
                     /translation="MEHLVTPDLPHEAGKITDDIRPVIARSFIRDAFDSITRGRPGYH
                     IWMGILTFFMLAGAYAYSVQLSEGLAVTGMNDRVSWGFYISNFTFLVGVAAAAVMLVL
                     PTYILKDVDFSKAVLIGEGLAVSALIMCLAFVTVDLGGPGRAWHLIPGIGRFNWPSSM
                     LTWDVLVLNGYLFLNLTIPFYILMCHYQGKHPNPKVYVPGVIISVFWAFCIHLVTAFL
                     YAGLPARPFWNSSLLGPRFLASAFAAGPALIIIVLSVIRRYTAYKVENVTLGKIALIV
                     TVAAQINLIMLGSELFKEFYKPTHHSESAVYLFFGLHGFGALKPWIWTAIGLNLIATA
                     ILTMNPLRSNHKWLLPACALLFLSIWIEKGMGLIIPGFIPGPWGKIVEYAPTWVEIFV
                     ALGIWAMGMFIFTLLVKIAIPIETGVLRYQPVKPSQTKN"
     gene            1412..1753
                     /locus_tag="PK754_13625"
     CDS             1412..1753
                     /locus_tag="PK754_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNB10590.1"
                     /translation="MSRVTKKNTPKRAIPKRFQRFLKEYPEVGKAYSALSHAVQDAGP
                     LDEKTRTLIKIGISAGSRQEGALHSHVRKALDAGATPEEIRHTILQCLPTIGFPGMMA
                     AMSWADDILNG"
     gene            2032..3033
                     /locus_tag="PK754_13630"
     CDS             2032..3033
                     /locus_tag="PK754_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB10591.1"
                     /translation="MPGTVRIEVLTSEVLQNNPLNDPHQRKLAVYLPPSYTTAPSRRF
                     PVIYLLSGFMGYGTMMLSPQAWGYAIDERCDKLIAEHAMEECIVVMPDCFTRWGGAQY
                     MNSPATGNYEDYIIREIIPYIDRTFRTQTERNHRGIMGKSSGGFGALRLAVNYPELFS
                     AFFCSAGDMYFEYGYKPDIPKTYNALYKTGGLDGFLKKFFEAPKKSGEQITAINIIAM
                     AAAYSPQASEPYGIELPFDLQTGALKESVWQRWIAHDPVFCVDQKAWRENLSKINHIF
                     LDCGARDEYHLHIGARIFADKLRNYSIPHWYEEFDDGHMGTSYRYDVSLSKLSRSIA"
     gene            3030..>3956
                     /locus_tag="PK754_13635"
     CDS             3030..>3956
                     /locus_tag="PK754_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10592.1"
                     /translation="MKSLFFIFVFGLSGLVSAQSLPVTLWLDVQIQEPLASDSNAWTA
                     RALHRSLVPALDKSSYFKIHPSANGAPEYYDGVIRVVCGYEKNELRFQMTVRDTLHSL
                     QKDFSYKTKPGQLRATHEKMMTDIFSSFNIADKKALETVKKDLQNIRSDEAYTMLQQA
                     FAAWSDTRTADSWIQKALQKEPRTSLLYERAGELAAARNEYDAAISYYHKTLELDSAR
                     FYIYRKIADVHYDHKNNLKSAKANYFEATHRDPTDAASWLQLGYCAYLEKDYTIARNY
                     ANKAVEVWQKKFGKTVYRETATAWNLIGNVAVA"
BASE COUNT         1017 a          964 c          898 g         1077 t
ORIGIN      
        1 tgctcgaccc ttccagcgaa atccgctggg ttcttgctaa taaaaaagtt tttcgactaa
       61 aagaagattt gggaactgaa cccaaattct ggtacttcat ggattagact atggaacatc
      121 ttgttacgcc cgatctgccg catgaagcgg gaaaaataac cgatgacatc cgtcccgtca
      181 tcgcgcgttc gtttattcgc gacgcgtttg acagcatcac acgcgggagg cccggttatc
      241 atatctggat gggcatcctg acatttttta tgttggccgg cgcctatgct tactccgtgc
      301 agttaagcga aggcttggcc gtcacgggta tgaacgatcg cgtgagttgg ggtttttata
      361 tttccaattt cacctttctt gtcggtgtgg cggcggccgc ggtgatgctg gtgttaccga
      421 cgtacattct caaggatgtt gatttttcta aagccgtcct gatcggtgaa ggtctcgccg
      481 tttcggcgct gatcatgtgt cttgcttttg tgacagttga cctcggcggc ccgggtcgtg
      541 catggcactt gatccccggc atcggtcgct tcaactggcc aagctcaatg cttacgtggg
      601 acgtacttgt actcaacggt tatttatttc tcaatcttac aatcccgttt tacattctga
      661 tgtgccatta tcagggaaag catcccaatc ccaaagttta tgtacccggt gtcattatca
      721 gcgtattctg ggcgttctgt attcacttag ttacggcatt tctgtacgcc ggtttgccgg
      781 cgcgaccgtt ttggaattca tcgcttttag gtccgcgatt tttggcgtcg gcttttgcag
      841 ccggtccggc gctaatcatc attgttttat ccgtcatccg ccgctatacg gcatataagg
      901 ttgaaaatgt aacactggga aaaatagcac tgatcgtaac cgtcgccgca caaatcaatt
      961 tgatcatgct cggttccgaa cttttcaagg aattttacaa accgacgcat cacagcgaaa
     1021 gcgccgtcta tctgtttttc ggattacacg gtttcggtgc gctaaagccg tggatatgga
     1081 cagccatcgg gttaaatctt attgccaccg ctattcttac aatgaatccg ttacgcagta
     1141 atcacaaatg gcttttgccc gcatgcgctt tgctttttct aagcatctgg atcgaaaaag
     1201 gaatgggttt gatcattccg ggatttattc ccggaccgtg gggaaaaatc gtcgaatatg
     1261 ctccgacgtg ggttgaaatt ttcgtcgcgc tcggtatctg ggcgatgggc atgtttattt
     1321 ttacattgct cgttaaaatc gctattccca tcgagactgg agttctgcga tatcagcctg
     1381 tgaaaccttc acaaaccaaa aattagaatg tatgtcacgc gttacaaaaa agaatacacc
     1441 aaaacgtgct atcccaaaac gttttcagcg ttttctgaag gagtatcccg aagtcggtaa
     1501 agcctattcg gcattgagtc atgccgtaca agatgccggc ccgctagatg aaaaaacccg
     1561 aacgttgatc aagatcggta tttctgctgg ttcgcgtcag gaaggagcgc tccattccca
     1621 tgtacgcaaa gcgcttgatg caggcgctac gccggaagaa attcgccaca ccattctcca
     1681 atgccttcct accatcggtt ttccaggcat gatggccgcc atgagctggg cggatgatat
     1741 tttgaacgga taaacctatc aaaaaaactt cgccgaaatc cgtccgcact aactttgctt
     1801 cttttatttt catcatgatt ttttgatcta atgttttatt atgtggcttc aaaaatttgc
     1861 cactcaaaca gaatccaatc caacccttaa actactctaa tctcaattgc ctacctcttt
     1921 gcagtaaaca tcaaagcgca aaaatacttt actatgtcgt aacggtatgg cactgcactt
     1981 gattatctga aaaactatcc tcagattcct ccaaaaacaa agagaaaaag aatgcccggc
     2041 accgtacgca ttgaagtttt gacaagcgaa gttttgcaaa acaacccgct caatgatccg
     2101 caccagcgca aattggccgt gtatttgccg ccttcctata cgacagcccc cagccggcgg
     2161 ttcccggtta tttacctctt gagcggattt atgggctacg gtacgatgat gctgtcaccg
     2221 caagcctggg gttatgcgat agacgaacgt tgtgataagc tcatcgccga acacgcgatg
     2281 gaagaatgca tcgtcgtcat gccggattgt tttacccgtt ggggcggtgc gcagtatatg
     2341 aattcgcccg ctaccggcaa ttatgaagat tatatcattc gcgaaatcat cccttacata
     2401 gatcgtacat ttcgcacaca gaccgagcga aatcaccggg gtatcatggg caaatccagc
     2461 ggcggatttg gtgcgctgcg tcttgccgta aactacccgg aattattttc cgcttttttt
     2521 tgcagcgccg gcgatatgta tttcgaatac ggatacaaac ccgatattcc caaaacctac
     2581 aatgccctgt ataaaaccgg tggtctggac ggatttttaa aaaaattttt tgaagcccca
     2641 aaaaaatccg gcgaacagat cacggctatc aatatcatcg ccatggcagc ggcttattcg
     2701 ccgcaagcat ccgaacctta cggtatcgaa ttgccttttg atctgcaaac cggcgcgctc
     2761 aaagagtccg tatggcaacg ctggatcgcc cacgatccgg ttttttgcgt ggatcaaaaa
     2821 gcctggcgcg agaatctttc taagatcaat catatttttt tagattgtgg cgcccgtgat
     2881 gaataccatc ttcatatcgg tgcgcgtata tttgcggata aacttcgtaa ctatagtatc
     2941 ccgcattggt atgaagaatt tgacgacggc cacatgggta cgagttaccg ttatgacgta
     3001 tcactttcca aattaagccg gagtattgca tgaaatcatt gttttttatt tttgtttttg
     3061 gcttatccgg tttggtatcc gcgcaatcgc tgccggtaac gttgtggctg gatgtacaaa
     3121 tccaagaacc gctggcttcc gactccaatg catggacggc acgggcgctc caccgttctc
     3181 tggtacccgc cttggataag tcctcttatt tcaaaatcca tccgtccgca aacggagcac
     3241 ccgaatatta tgacggcgtc atccgggttg tgtgcggtta tgaaaaaaat gaacttcgct
     3301 ttcagatgac ggtgcgcgac acactgcaca gccttcaaaa agatttttcc tataaaacca
     3361 aaccgggaca acttcgtgcg acgcatgaaa aaatgatgac cgatattttt tcatccttta
     3421 atatcgccga taaaaaagcc ctcgaaacgg taaaaaaaga tttacaaaat attcgctccg
     3481 atgaagcgta cacgatgctt cagcaagcgt ttgccgcatg gtcggatacg cgaaccgcag
     3541 acagctggat acaaaaagcg ttacaaaagg aaccgcgcac ttcgctcttg tacgaacgcg
     3601 ccggtgaatt ggccgcggca cgcaacgaat acgatgccgc tattagttat tatcacaaaa
     3661 ctcttgagct ggattcggcg cgtttttata tctatcgaaa aatagcggat gtgcactacg
     3721 atcataaaaa caatctcaag tcggcaaagg ctaattattt tgaagctacg caccgcgatc
     3781 cgacagacgc tgcaagttgg ttacaattgg gttactgcgc ttatctggaa aaagattata
     3841 cgattgcacg taactacgcc aacaaagccg tcgaagtatg gcagaagaaa ttcggtaaaa
     3901 ccgtttaccg ggaaaccgct acggcatgga atctgatcgg caatgtcgcg gtcgcc
//
LOCUS       DAOQNQ010000295         3954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_10509_length_3954_cov_5.469608, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000295 DAOQNQ010000000
VERSION     DAOQNQ010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10509_length_3954_cov_5.469608"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PK754_13640"
     CDS             <1..287
                     /locus_tag="PK754_13640"
                     /inference="COORDINATES: protein motif:HMM:NF024768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transport complex protein RnfC"
                     /protein_id="HNB10593.1"
                     /translation="GRRTPLKMLVKRLGVTDYDIHAPYSDRQATPFKRIILPLQQHVG
                     QKAEAVIRTGESVKRGQLIADVPQDKMGAPIHASIDGTARVEADHILIES"
     gene            311..862
                     /locus_tag="PK754_13645"
     CDS             311..862
                     /locus_tag="PK754_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HNB10594.1"
                     /translation="MTMNSIGLIELGSIAAGFDTADAMLKAANVNILLARSICSGKYI
                     VMVGGEVAAVQSSIDAGAAQSRGSVIDTFVIPNVHPEVFPALSGAGRSELRESLGIIE
                     SFSVASLIEAADAAVKAANVKLLEIRLAMALGGKAFLTMTGEVAAVQAAVDAGSRVVA
                     EKGLLVNKVVIPGPRPELLTEVV"
     gene            962..1750
                     /locus_tag="PK754_13650"
     CDS             962..1750
                     /locus_tag="PK754_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB10595.1"
                     /translation="MNLNLQNRTALVCGSTQGIGKAAAMALAGLGARIILCARNAEAL
                     ENVKNELPQPASAHHTYLVADFSKPETLQQILHTFTEKSGPIHILVNNTGGPAGGPIA
                     DAATDAFEQTFRQHLICNHILAQAVIPGMKSSGFGRIINVISTSVKQPLKGLGVSNTI
                     RAAVANWAKTLSVELAPFGITVNNVLPGATLTQRLTTLIANRAKQSGRPEEEIQKEMV
                     AEIPAGRLGQAEDIAAAIAFLASPAAGYINGINLPVDGGRTGCL"
     gene            1754..2338
                     /locus_tag="PK754_13655"
     CDS             1754..2338
                     /locus_tag="PK754_13655"
                     /inference="COORDINATES: protein motif:HMM:NF016932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HNB10596.1"
                     /translation="MISAPEPSELLRKVFAAHTENYGDPDVQFHFDQQNSTLTALNYL
                     DVFVWEPTEEIPMTTFSTIGMAERAMPECPHRAEIHWTLRGKLTEAQEEEAAVFLLNL
                     AALPFVKNTFLDHWHVLPNLRIPHFSGCAAGLMHPSFVDGGWDHMHAENTIVKFLNFV
                     PLTQSELDMARTMGVRRMLNALYESQTDIFSDRM"
     gene            2351..3235
                     /locus_tag="PK754_13660"
     CDS             2351..3235
                     /locus_tag="PK754_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNB10597.1"
                     /translation="MEELLQIVSDAYAGYSNYLYMEMTGASVPWYRNYFYGLIAISLV
                     VWAFEIIIPWRKNQPVFRRDFWLDGFYMFFNFFLFSLIAYNALSNVCVVWLDRGLETI
                     GIGSLSFIKVNTWPVWLQYILMFVVADFIQWNIHRLLHRVPWLWEFHKLHHSVKEMGF
                     AAHLRFHWMETVIYKSLQYIPLAFFGFGLTDFFMLHMIALAIGHLNHANIAWTWGPLR
                     WLLNSPVMHIWHHAKHLPGSYPYGVNFGLSLSVWDYLFKTAYIPHDGRDIELGFENEE
                     PFPRGFFKQVIYPIGKQN"
     gene            3338..>3954
                     /locus_tag="PK754_13665"
     CDS             3338..>3954
                     /locus_tag="PK754_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002615569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNB10598.1"
                     /translation="MKTISNYIDGKLQPALSGKTLDNYEPAVGAVYGTIPDSDERDVA
                     MAVDAAEKAFPAWSLMPAEQRARLLFKLSELIDRDMEKLVRAESIDNGKPLTLARAMD
                     IPRASSNFYYFASAILQFSSEAHAMENTAINYTLRQPIGVVGCISPWNLPLYLFTWKI
                     APALAAGNCVVAKPSEITPMTAFMLSELAIEAGFPKGVLNIVHGLG"
BASE COUNT          956 a          999 c          988 g         1011 t
ORIGIN      
        1 aagggcgccg caccccgctc aaaatgctgg taaaacgtct tggcgtcacg gattacgaca
       61 tacacgcccc ttattccgat cgtcaagcaa ctccatttaa acgtataatc ctgccgctgc
      121 agcagcacgt cggacaaaaa gccgaagcgg ttattcgcac gggtgaatcg gtaaaacggg
      181 ggcagcttat cgccgatgtc ccgcaggata aaatgggcgc accgatccac gccagcattg
      241 acggtacggc acgcgtcgaa gcggaccaca tccttatcga atcttaaaat gtaaatttga
      301 ggatattatc atgactatga attctatcgg attgatcgaa ctcggcagta ttgcagcggg
      361 atttgacacg gcggatgcga tgctcaaagc ggctaacgtt aacatcttac tggcacgttc
      421 gatttgttca ggcaaataca tcgtgatggt gggcggtgaa gtcgcggcgg tgcaatccag
      481 cattgatgcc ggtgcagcac aaagccgcgg ttcggtcata gacacctttg tcattcccaa
      541 tgtgcatccg gaagtttttc ctgcgctctc cggagcgggc cgatccgaac tgcgcgaatc
      601 gctgggtatt atcgaatcgt tttctgtggc ttctttgatc gaagccgccg atgccgctgt
      661 caaagccgcc aatgtcaaat tgctggaaat tcgcctcgct atggcgctcg gcggtaaagc
      721 gtttctcacc atgaccggtg aagtcgccgc tgttcaggcc gctgtagatg cgggttcacg
      781 tgtcgtggcc gaaaaaggac tactcgtcaa caaagtcgtc ataccgggtc cgcgaccgga
      841 actgctgacc gaagtagtct gatcagataa ttttctctcg cggagacgca aagtacacga
      901 cgtcgttcca gtttgtatct ttttttcatc gcgtctttgc gagcaaatac tataaacaga
      961 catgaatctc aatctgcaaa atagaaccgc cttggtgtgc ggcagcacgc aaggcatcgg
     1021 taaagccgcc gcaatggcgc tggccggtct cggcgcgcga atcatacttt gtgcacgtaa
     1081 cgccgaggcg ttggaaaatg taaaaaacga actgccccaa ccggcttcgg cgcaccatac
     1141 gtacctggtg gcagattttt caaaaccgga gacgcttcag caaatcctcc atacgtttac
     1201 agaaaaatca ggtccgatac acattctcgt caataatacc ggcggtcccg cgggcggccc
     1261 gatcgcggat gcagcgaccg atgcatttga gcaaaccttt cgccagcatt tgatttgcaa
     1321 ccacatcctg gcacaagcgg taatccccgg tatgaaatca tcgggttttg gtcgtattat
     1381 caatgtgatc tcgacctccg tcaaacagcc gctcaaaggt ttaggcgtat ccaataccat
     1441 tcgcgccgca gtggccaact gggcaaagac attatcggtc gaacttgcac cgtttggcat
     1501 aacggtgaac aacgttttgc ccggcgccac cctcacacaa cgcttgacca ctctcatcgc
     1561 caaccgcgcc aaacaaagcg gtcgcccgga agaggaaatt caaaaagaaa tggtggccga
     1621 aattcccgcc ggacgcctgg ggcaagcgga agatattgct gccgccatcg ctttcctcgc
     1681 ctcacccgcg gccggatata tcaatggtat caatctgccc gtggatggcg gaagaacggg
     1741 atgcttataa atcatgatct ccgcgcctga accctccgag ctgctacgta aagttttcgc
     1801 tgcgcatacg gaaaattacg gtgaccccga tgtacagttt cacttcgatc agcaaaattc
     1861 aacgctgacc gcgctcaact acctcgacgt gtttgtttgg gaaccgacgg aagaaatccc
     1921 catgacgacg ttttccacca taggtatggc cgaacgtgca atgccggaat gtccgcatcg
     1981 cgccgaaatt cactggacgc tgcgcggcaa actgacggaa gcacaagagg aagaggccgc
     2041 tgttttttta cttaaccttg ccgcgctgcc ttttgttaaa aatacttttt tagatcattg
     2101 gcatgtatta cccaacctca gaattcccca ttttagcggt tgcgcggccg gattgatgca
     2161 cccttcgttt gtggatggtg gctgggatca catgcacgcc gaaaatacca tcgtaaaatt
     2221 tcttaatttt gtcccgctga cgcaaagcga attagacatg gcgcgtacga tgggagttcg
     2281 tcgtatgctt aatgcgttgt atgaaagtca gacggatatt ttttccgatc gcatgtgaca
     2341 gggagcgctt atggaagaac tgcttcagat cgtttcagac gcctacgcgg gttattccaa
     2401 ttatctttac atggaaatga caggggcctc cgtcccatgg tatcgtaatt atttctacgg
     2461 tctgatcgca atttcccttg tcgtatgggc atttgaaatc atcatcccgt ggcgtaaaaa
     2521 tcagcccgtg tttcgccgcg atttttggct cgatggcttt tatatgtttt ttaatttttt
     2581 tttgttttca ctgatcgcgt acaacgcttt atccaatgtg tgcgtcgtat ggctggatcg
     2641 cggattggaa actatcggta tcggcagtct gtcctttatc aaagtaaaca catggccggt
     2701 atggctacag tacatactta tgtttgtcgt ggcagatttt attcagtgga atatccaccg
     2761 cttactgcac cgcgttccgt ggctgtggga atttcacaaa ctgcatcact cggttaaaga
     2821 aatgggattt gcggcacatt tgcggtttca ctggatggaa acggtgattt acaaatcgct
     2881 tcagtatata cccctcgcgt tttttggttt tgggttgacg gattttttta tgctgcatat
     2941 gatcgcactt gcgatcgggc atctcaatca cgccaatatt gcatggacgt ggggtccgct
     3001 caggtggctg ctcaatagcc cggttatgca catctggcat cacgccaaac atcttccggg
     3061 atcgtatccg tatggcgtca atttcggatt atcactgagc gtatgggact atctttttaa
     3121 gacggcatac ataccgcatg acgggcgcga tatcgaactt ggttttgaaa atgaagaacc
     3181 tttcccgcgc ggcttcttta aacaagtaat atatcccatc ggcaaacaaa attgaaatat
     3241 gtacgcatca cacatctata ccttaaagaa gtgattttac taagcgaccg tatccgatgg
     3301 attttatttt ttactatgct aaccaaccgt gatttttatg aaaactatat cgaactacat
     3361 tgacggaaaa ctccaacccg cactaagcgg aaaaactttg gataactatg aaccggcggt
     3421 gggcgccgtc tatggcacga taccggacag cgatgaacgc gatgtcgcga tggccgttga
     3481 tgcggcggaa aaagcttttc ctgcgtggtc tttgatgccc gccgagcagc gtgcgcggtt
     3541 acttttcaaa ctttcggaac tgattgatcg ggatatggaa aaacttgtcc gcgccgaatc
     3601 catagacaac ggtaagccgc tgacccttgc gcgggcgatg gatataccgc gggcttcgag
     3661 taacttttat tattttgctt cggcgattct tcagttttcc agcgaagcgc atgcgatgga
     3721 aaatacggcg atcaactata ctttgcgtca gccgatcggt gtcgtgggtt gcatttcacc
     3781 gtggaatctg ccgttgtatc tttttacctg gaaaatcgcc ccggcgctcg cggcgggaaa
     3841 ttgtgtggtg gccaaaccgt cggagataac accgatgacc gcgtttatgc tgtccgaatt
     3901 agccatcgaa gcggggtttc cgaaaggcgt actgaatatc gttcacgggc tcgg
//
LOCUS       DAOQNQ010000296         3883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_10845_length_3883_cov_5.983020, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000296 DAOQNQ010000000
VERSION     DAOQNQ010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10845_length_3883_cov_5.983020"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PK754_13670"
     CDS             <1..403
                     /locus_tag="PK754_13670"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499260.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNB10599.1"
                     /translation="VAAGEKLPFQQSDFKPRGHAIECRINAEDPDHNFRPSAGKIHAF
                     HIPSGYGIRVDSHAYAEYQIPSNYDSMIGKLIVLGENRTEAISKMCGALDEFIIEGIK
                     TTIPFQKRMMLNENFVSGDFDTKFVERLLGL"
     gene            534..3821
                     /locus_tag="PK754_13675"
     CDS             534..3821
                     /locus_tag="PK754_13675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB10600.1"
                     /translation="MIRFGVIIKSLLIFATGYWLNPAPQNTYAQSLPDHFSQLTVENG
                     LSHSTIYTVYQDRLGFIWMGTENGLNAYDGYRFIHFKHDPRDSTTLPNNNVTSIVEDD
                     EARLWLGTWGGGLARFDRTTSTFKTYKPIAGLNHTLSDLRIQYILRRSDGKLWIGTNS
                     GGVNIFDPVTEKFVHVSHDPHSEASLPHPRVWSLAEQNHAVWVGTGNGLCRVSETPDG
                     LKTERIEKIYPGLVLSHGTIRALFADGDSLLWVGTQDGLDRVDLRTASVRRSLTHERV
                     NVVTRLQNGHLAAGTIQHGMFIMDSDGTVIKTYHHDPNYAHSLGHNDVRWIMEDRSGI
                     VWIATRGEGVSLLDSKPPKFSIVADDIQNNTLRSTRVAAVFEDHSANGKLVLAGTDRG
                     LIVYNEKKRSFTTYLEPTEDNTSSVWITSIYKDPAGFYWIGTFGEGLIWFDLQRGVLK
                     KIVSRPGDSTSLNDNRVRTIYAQSINGVRRLWIGTYGGLHLWDESNNRFKRWVAQNGN
                     PNSLSHNFIRTIVSDHVNRLWIGTYGGGLNRFEPETNNWKHFKGNPTYKNSISGNEIM
                     TIALDPDTAHPHLWIGTDGNGISLMDIRHETFRSISIADGLPSDAVYNIIPTGLDHFW
                     ISTNNGLSQLRYIPDNQFAFRNFNAVDKLPSNIFAQGAHYLSNDGTLYFGGVHGIVSV
                     NTRKLVFNQIAVKPQITGYTLHNGESTIERSTFGLDELTLESSVTWLTFQFSALEYTN
                     SLKNKYAYKLDGLDEHWIYSDERRSASYNTLAPGDYTFLIKASNNDGVWCESPTRLTL
                     HVLPPFWKTWYFRLAIVVLITLALWSFHRLRLQRVEQYNRRLEKIINERTTELKLKNE
                     ELEVLNQKKNEFLGIAAHDLRNPLTTIIGYVQLMINDIRHGHATMKDVENDLDTVHKV
                     SQQMASLVSTLLDWSSIEAGKVNLEKHSESLQRIIDECENLHRRAAEQKNIRLAVERP
                     LLMPNVFVDRNRILEVMDNLLSNAIKYTHPGGAVRVFCEPTDREICVHVEDTGQGLSQ
                     EDLNKVFKSYQKLSARPTAGEVSTGLGLAIVKKVIELHGGRVWVTSEKGKGSRFSFSV
                     PRYTEYN"
BASE COUNT         1103 a          891 c          868 g         1021 t
ORIGIN      
        1 cgttgccgcc ggagaaaaac tgccgttcca gcagtcggat ttcaaaccgc gcggacacgc
       61 gatcgaatgc cgcattaacg ccgaagatcc cgatcacaat ttccgtccgt cagccggaaa
      121 aatacatgcg tttcatattc cgagcggtta cggtattcgt gttgattcgc atgcgtatgc
      181 cgaatatcaa atcccgtcca actatgactc gatgatcggt aaactgatcg ttttgggaga
      241 aaaccgtacc gaagccatca gcaagatgtg cggggcattg gacgaattta tcatcgaagg
      301 catcaaaact acgatcccct ttcagaaaag gatgatgctc aacgaaaatt tcgtgagcgg
      361 agattttgat acgaagtttg tggaacgcct gctcggtttg tagtatattg catccactgt
      421 aaagtatgtc tatattcata ccgggactgt aatccgtccc ggtatggttc ttattataaa
      481 atatagccga agttccgatt gccattggtc atccttgcta caaaagggag ttcatgattc
      541 ggttcggtgt catcatcaaa tctctcctta tttttgctac gggttattgg cttaatcccg
      601 caccgcagaa tacgtacgca caatccttac ctgatcattt tagtcaactc acggtcgaaa
      661 acggtttatc ccacagcacc atttatacgg tctatcagga ccggctcggc ttcatctgga
      721 tgggtactga aaacgggctc aacgcctatg acggctatag atttattcat tttaaacacg
      781 atccgcgtga ttcgaccacg ctgcccaata acaatgtgac ttccatcgtc gaagatgacg
      841 aagcgcgttt atggcttggt acttggggcg gtggtttagc gcgatttgac cgaaccacct
      901 ctacatttaa aacatacaaa ccgatagccg gtctaaatca taccctgagt gacctacgca
      961 tacaatacat cttacgtcgc tccgatggca aactatggat cggtaccaat tccggtggag
     1021 ttaatatttt tgatccggta actgaaaaat tcgtacacgt gtcgcacgac ccgcatagcg
     1081 aagcgtcatt accgcatccg cgtgtgtggt cattggctga acaaaatcac gccgtgtggg
     1141 ttggcaccgg taacggcctt tgccgcgtgt cggaaacacc cgatggatta aaaacagaac
     1201 gcatcgaaaa aatttatccc ggtcttgtac tgagtcacgg tactattcgt gcgttatttg
     1261 cggatggaga cagtcttttg tgggttggca cccaagacgg tttggaccgc gtagatctgc
     1321 gcacggcatc agtacgacga agtttaacac acgaacgcgt taacgtcgtc acacgtttac
     1381 aaaacggtca tctggccgcc ggtaccattc agcacggaat gtttattatg gatagcgatg
     1441 ggacggttat caaaacttac catcacgatc cgaattatgc ccattcgttg ggacataatg
     1501 atgtacgttg gattatggaa gatcgctccg gaatagtatg gatcgcgaca cgcggtgaag
     1561 gcgtcagttt attagatagt aagccaccaa aattttccat cgtcgctgac gatatccaaa
     1621 acaacacgct ccgaagcaca cgggtcgccg ctgtatttga agatcatagc gccaatggaa
     1681 aattagtact ggccggcacg gaccgcggtt tgatcgtgta caatgaaaag aaacgatcgt
     1741 ttacaaccta tcttgaacct acggaagata acacatcgtc ggtatggatc acttctatct
     1801 acaaagaccc agccggtttt tactggatcg gcacattcgg cgaaggtttg atttggtttg
     1861 atttacagag aggtgttctt aaaaaaatcg taagccgacc gggagactcc acctcattga
     1921 acgataatcg tgtacgaacc atttatgcac aatccataaa cggtgttcgc cgactatgga
     1981 ttggtacgta tggaggatta catctttggg acgaatcgaa taatcgtttt aaacgttggg
     2041 ttgcacaaaa cggtaatccg aacagtttaa gtcacaattt tattcgtacc attgtatctg
     2101 atcatgtaaa tcgtttgtgg atcggcacct atggcggcgg actcaacaga tttgaacctg
     2161 aaaccaacaa ttggaaacat tttaaaggaa acccgacata taaaaatagc atttcgggaa
     2221 atgaaattat gaccatcgca ttggatccgg atacggctca tccgcatctt tggatcggta
     2281 cggacggcaa tggtataagt ttgatggata ttcgacacga aacatttcgt tccatttcca
     2341 tagctgacgg tttaccgtcc gatgccgtat ataatattat tcctaccgga ctggatcatt
     2401 tttggatatc taccaataac gggctctcgc aactgaggta tataccggac aatcagtttg
     2461 cttttagaaa ttttaatgcc gtggacaaac ttccttccaa tatttttgca caaggtgcgc
     2521 attatttatc aaacgacggg acgctttatt tcggcggtgt acacggcata gtttcagtca
     2581 atactcgcaa gttggttttt aaccagatcg ctgtaaaacc tcagattacc ggctatacgc
     2641 tgcataacgg tgaatctacc attgaacgtt cgacatttgg attagacgaa cttaccctcg
     2701 aatcatccgt cacatggctt acatttcagt tcagcgcgct cgaatacacc aattcactaa
     2761 aaaataaata cgcctataaa ctcgacggct tggacgaaca ttggatttac agcgatgaac
     2821 ggcgttcggc atcgtataac accctcgcac ccggcgatta tacgtttttg ataaaagcat
     2881 ccaataacga cggcgtatgg tgtgagtcac cgacacgact cacattgcat gttttaccgc
     2941 cattctggaa aacttggtat tttcgtttgg ccatcgttgt tttgatcacc ttggcgttgt
     3001 ggtcgtttca tcggctgcgc cttcaaagag tggagcaata caaccgtcgc cttgaaaaaa
     3061 tcatcaacga gcgtacgacg gaattaaaat taaaaaacga agaactggaa gtcctcaatc
     3121 aaaagaaaaa tgaattcctc gggatcgcgg cacacgatct ccgaaaccct ctgacaacaa
     3181 tcatcggcta tgtacaactg atgatcaatg atatccggca tggacacgct acgatgaaag
     3241 atgtggagaa tgatttagat actgtgcaca aagtatcaca acaaatggca agtcttgttt
     3301 ctactttatt ggattggagc tccatcgaag ccggtaaagt caacttagaa aaacacagtg
     3361 aaagtttgca gcgcatcata gacgaatgtg aaaatctgca tcgtcgtgct gctgaacaaa
     3421 aaaacattcg attagctgtc gagcggcctt tactcatgcc taatgtattt gtagatcgca
     3481 accggatttt ggaagtgatg gataatttgc tctccaatgc gatcaaatac acgcaccccg
     3541 gaggtgcagt gcgcgttttt tgtgaaccaa cagatcgcga aatctgcgtt catgtcgaag
     3601 atacaggaca aggcctctca caagaagacc ttaacaaagt attcaaatcg tatcagaaac
     3661 tcagcgcacg tccgacggcc ggtgaagtca gcacagggct tggtctggcg atcgtcaaaa
     3721 aagtaatcga attgcacggc ggacgcgtat gggtaaccag cgaaaaaggc aaaggttcgc
     3781 gatttagttt ttctgttcct cgctatactg agtataatta aatgtaattt catcctcgct
     3841 ttacacatca tacaaagttt aactcccggc cacggcacca tat
//
LOCUS       DAOQNQ010000297         3879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_10869_length_3879_cov_5.571391, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000297 DAOQNQ010000000
VERSION     DAOQNQ010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10869_length_3879_cov_5.571391"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PK754_13680"
     CDS             <1..219
                     /locus_tag="PK754_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10601.1"
                     /translation="SPHEEFDDAPADFDKQAFAYRTILETYQNKPWFKGYFWWKWESA
                     NSELKRFDKMLQFRGKPAERVLQEFYSR"
     gene            complement(442..885)
                     /locus_tag="PK754_13685"
     CDS             complement(442..885)
                     /locus_tag="PK754_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNB10602.1"
                     /translation="MGNTIRLHRVLKATPEKIYRAFTDADAMARWLPPHGFTCKVHHM
                     DAKIGGTFRMSFTNFTTMKSHAFGGEFLELTPYERIRYTDKFDDPNLPGEMMVTVVLK
                     KVLCGTELNISQENIPEIIPPEMCYLGWQESLSYLAHLVEPEIQE"
     gene            complement(1061..1771)
                     /locus_tag="PK754_13690"
     CDS             complement(1061..1771)
                     /locus_tag="PK754_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB10603.1"
                     /translation="MRILVFGANGATGKLVVSQLVNKNISVRIVIRPTALIPSEISEN
                     KNVEIVRGNIHEFSMDQVSALIDDCDAVVSCLGHNLTFKGIFGKPHQLVFSIVKKITD
                     VWSASQNKKKFVLMSTTGYTNKAHGEKETFGENVVFSLLKLLLPPHTDNVNAGDYLFG
                     RVKDSKQLEWVAVRPDSLIDEMRVSEYEIVDTRKRSPIFDAGKTSRVNVAHFMVEPLT
                     DDALWEAWKYKAPVLYNK"
     gene            complement(1974..>3879)
                     /gene="katG"
                     /locus_tag="PK754_13695"
     CDS             complement(1974..>3879)
                     /gene="katG"
                     /locus_tag="PK754_13695"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018213251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HNB10604.1"
                     /translation="AGTYRIADGRGGAGNGTQRFAPLNSWPDNANLDKARRLLWPIKQ
                     KYGNKISWADLMILAGNCALESMGFKTFGFAGGREDIWEPEEDIYWGSETEWLGDKRY
                     SGERDLENPLGAVQMGLIYVNPQGPNGKPDPVASGRDIRETFARMAMNDEETVALVAG
                     GHTFGKAHGAAPDSHVGREPEAASIEEQGLGWTSKYGSGKGDDTITSGIEGAWKPNPT
                     QWDNGYFDMLFGYDWELVKSPAGAWQWTPKNVADKDLAPAAHDATKRVPTMMTTADMA
                     LRMDPIYEPISRRFHKDPQAFADAFARAWFKLTHRDMGPRSRYLGEEVPKEVLIWQDP
                     VPAADHKLVNEKDIAALKKKISESKLTVSQLVSTAWASASTFRGSDKRGGANGARIRL
                     APQKDWAINQPEQLSAVLNKLEKIQKEFNRAQPGGKKKISLADMIVLGGCIGIELAAK
                     QAEQNITVPFSPGRTDATQAQTDAASFAVLEPVADGFRNYLKADYDVPAEELLIDKAQ
                     LLTLTAPEMTALIGGMRVLNVNHGQTKHGVFTHRPETLTNDFFVNLLDMRTAWVASPG
                     AKGVFEGRDRTTGELRWTGTRVDLVFGSNSQLRALAEVYAQSDAQKKFVQDFVAAWTK
                     VMNADRFDLA"
BASE COUNT         1004 a          991 c          798 g         1086 t
ORIGIN      
        1 agtccgcacg aagaatttga tgatgcccct gcggattttg acaagcaggc tttcgcttac
       61 cgtacgattt tagaaacata tcagaataaa ccctggttca aaggttactt ttggtggaaa
      121 tgggaaagcg ccaactctga attaaaaaga tttgataaaa tgctgcaatt ccgaggaaaa
      181 cctgccgaac gcgtgttaca ggaattttat tcgcgttaat ttaacataag cgtgaaccta
      241 agtgaaacaa cggactgtcc gtattagaat tcatctctca ccaagacgct aagccccaat
      301 aaaaagattt attaattact ctgcggcttt gcgagaataa tacaagctta tctaataaaa
      361 ctcattacac caaattctaa gggcgcgggt aagaacttca acgacgcacc aagagatccg
      421 tacttgtaaa attgccttca attactcctg aatctcaggc tctaccagat gcgcaagata
      481 cgacaaagac tcctgccaac ctaaataaca catttcaggc ggaatgatct ccggtatatt
      541 ttcttgcgat atgttcaatt ccgtaccgca caaaactttt tttaaaacca ccgtaaccat
      601 catttcgccg ggaagattgg gatcgtcaaa tttatccgtg tagcgaatac gttcatacgg
      661 tgtaagctcc aaaaattcac cgccaaacgc atgacttttc atcgttgtga aattagtaaa
      721 cgacattcta aacgtaccgc ctatttttgc atccatgtga tgtactttgc acgtgaatcc
      781 gtgcggcggc agccagcgcg ccatcgcatc ggcatctgta aatgcgcggt agattttttc
      841 cggagtagcc ttcaaaacgc ggtgtagacg aatcgtgttt cccatatcaa ctccttttgt
      901 tatacaagat tcaaattaaa cctgtactct ttatatcact gtcatccata aatctgatac
      961 tttatcaccg agcgaaataa catccgccga gggttatagc gtttgacgta cagtctcttt
     1021 ttttatccta aaaatcaaaa cttcgccgaa ctttttacat ttatttgtta tacaataccg
     1081 gtgctttgta tttccatgct tcccataatg catcgtcggt caggggctcg accataaaat
     1141 gcgccacatt gactcgactc gttttaccgg catcaaatat cggactacgt tttctggtat
     1201 caacgatctc atattcgctg actcgcattt catcaatcag tgaatccggt cgaaccgcaa
     1261 cccattctaa ttgttttgaa tcttttacac gaccaaacaa ataatccccc gcattcacat
     1321 tatcggtatg cggcggcaaa agtaatttta acaacgaaaa cactacgttt tctccaaacg
     1381 tttctttttc accgtgtgct ttatttgtat atcctgtagt gctcatcagc acgaatttct
     1441 ttttgttttg cgaagcgctc cacacgtcgg tgatcttttt tacgatacta aaaacaagtt
     1501 gatgcggttt accaaatatc cctttgaatg tcaaattatg accgagacaa gacaccaccg
     1561 catcacaatc gtctatgagc gcactgacct gatccataga aaactcatga atattccctc
     1621 gtacgatttc tacgttttta ttctcagaaa tctcactcgg tattagcgcc gtcggtcgta
     1681 taacaattct cacggatata ttcttgttaa ccaattgtga tactaccaat ttccctgtag
     1741 ccccgttggc tccgaatact aaaattctca taaaaccacc tctctgctat ttataaatcc
     1801 gagggcaata taagaaatag ttttattgat aggttaactt ctttgtgcga tgattttcgc
     1861 agatacccaa gatgttactg attagctgca tggcattttt actgaataaa aaaaccggca
     1921 agctacatat aacacttgcc ggttatctca attagattat cgtctggcca atatcatgca
     1981 agatcaaagc gatcggcgtt catcacttta gtccaagcgg ccacaaaatc ctgtacgaat
     2041 tttttctgcg catcactttg tgcatatact tccgccaatg cgcgtaattg ggaattggaa
     2101 ccaaaaacca aatctacgcg agtaccggtc caacgcaatt cgccggtcgt acgatcgcgt
     2161 ccttcaaata cgcctttagc gcccggagag gccacccacg ccgtacgcat atctagcaga
     2221 ttaacaaaaa aatcattggt caatgtttcc ggacgatgcg taaacacgcc gtgtttggtt
     2281 tgaccatgat tgacattcaa tacacgcata ccgccgatga gcgccgtcat ttccggtgca
     2341 gtgagtgtca gcaattgcgc tttatctatc agaagttcct cggccggcac atcgtaatcg
     2401 gctttaagat aatttcggaa tccgtcggcg accggttcga ggacggcaaa agaagcggca
     2461 tcggtttgcg cttgcgtagc gtccgtgcgt cccggcgaga aaggtaccgt gatattttgc
     2521 tcggcttgtt ttgcagccaa ttcgataccg atacaaccgc caagcacgat catatccgcg
     2581 agcgaaattt tcttcttccc tccgggttgt gcgcgattga attctttttg aattttttcc
     2641 aatttattca ataccgcgct caattgttcc ggttgattga tcgcccaatc tttttgcggc
     2701 gcaagacgga tgcgtgcccc attggcgccg ccgcgtttat cagagccgcg aaatgtcgaa
     2761 gccgaagccc atgcggtgga taccaactgc gagacggtca atttagattc gctgattttt
     2821 ttcttcagcg ccgcgatatc tttttcatta actaatttat gatcagcggc ggggacaggg
     2881 tcctgccaga tcagaacttc tttgggtact tcttcgccga gataacgtga acgcggaccc
     2941 atgtcgcggt gcgtgagttt gaaccatgca cgcgcaaaag catcggcaaa tgcttgcggg
     3001 tctttgtgaa agcgacgtga aatcggttca taaatgggat ccatgcgcaa cgccatatcc
     3061 gccgtggtca tcatcgttgg cacacgtttg gtcgcatcgt gtgcagcagg cgcaagatct
     3121 ttatccgcta cgttttttgg tgtccactgc catgcaccgg cagggctctt gaccaattcc
     3181 caatcataac caaacaacat atcaaagtat ccgttgtccc actgcgtcgg attgggtttc
     3241 caggcgcctt cgataccgct ggtgatcgtg tcatcgcctt tgccgctacc atatttactg
     3301 gtccatccaa ggccttgttc ttcgatactc gccgcttccg gttcacggcc gacatgggaa
     3361 tccggcgccg cgccgtgcgc ttttccgaac gtatgtcctc ctgcaacaag cgccacagtt
     3421 tcttcgtcat tcatagccat acgcgcaaat gtttcgcgaa tatcgcgccc cgaagcgacc
     3481 ggatcgggtt taccgttggg tccttgcgga ttaacgtaga tcaatcccat ttgtacagcg
     3541 ccaagagggt tttccaaatc gcgctcgcca ctataacgtt tatcgccgag ccattcggtc
     3601 tcggaacccc aataaatatc ttcttcgggt tcccatatat cttcgcgccc accggcaaaa
     3661 ccaaaagttt tgaagcccat cgattctagg gcgcaattac cggccaaaat catcagatca
     3721 gcccaggaaa ttttgtttcc atatttttgt ttgatcggcc acagcaaacg tcgcgctttg
     3781 tccaagttgg cattgtccgg ccagctgttg agcggtgcaa aacgctgcgt accgttaccg
     3841 gcgccgccgc gtccgtccgc aatgcgatac gtaccggcg
//
LOCUS       DAOQNQ010000298         3874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_10901_length_3874_cov_5.592302, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000298 DAOQNQ010000000
VERSION     DAOQNQ010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10901_length_3874_cov_5.592302"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            375..1499
                     /locus_tag="PK754_13700"
     CDS             375..1499
                     /locus_tag="PK754_13700"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HNB10605.1"
                     /translation="MVIGIPKETAAGENRVAVTPDTAAKLIKMGFEVRVEKGAGQNAH
                     YTDEQYTKHGAAIAATATDLLGQSDIILKVQKPSDAEIKQIKKGALLISFVYGLNYPQ
                     VPKACAEAGVNLMSMDFIPRTTLAQKMDALSSQYNIAGYKAVLMCAAAFGKMFPLMMT
                     AAGTISPAKVVIMGAGVAGLQAIGTARRLGAVVEVSDVRAAVKEEVQSLGGKFIEVPG
                     AENMQDAGGYAKEASAEFLQKQKELLFKHITEADIVITTALVPGKKAPVLVTEEMIKH
                     MRHGSIVLDMAVEFGGNCEVSEAGKTVTKHGVTIIGESNIPATVPTHASELYSKNLLA
                     LLQYIAKDGNLQLKLEDEIVKSALLVHNGEVVNERIKQVL"
     gene            1521..1799
                     /locus_tag="PK754_13705"
     CDS             1521..1799
                     /locus_tag="PK754_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015486586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNB10606.1"
                     /translation="MGDIVMLITVFVLACFVGVELISKVPPTLHTPLMSGSNAISGIT
                     IVGSLISAGIGEMTISTIMGVIAVAFAMINVVGGYLVTDRMLKMFKRK"
     gene            1830..3272
                     /locus_tag="PK754_13710"
     CDS             1830..3272
                     /locus_tag="PK754_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017743022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNB10607.1"
                     /translation="MEHFRESIENIMYLIGAVLFIFGIKMLGSPKTARKGNFYSAVGM
                     LIAIVVTLFDKHILTYEWIIVGILAGSVIGIIFAVKVEMTSMPQMVGLLNGFGGGAST
                     LVALAEYYRGMATYQTALQSNPSLTLLEHMPYQTTVSIVLGILVGAVTFNGSLVAYAK
                     LQGLISGNSIRYPMQHPLNALMIVVTLVIGALGVMDLNNQWYFLTVTILGLILGITLV
                     IPIGGADMPVVISLLNSYSGIAAAITGFVINNNMLIITGALVGASGIILTDIMCKGMN
                     RSLMNVLLGGWGTAPTVEASKDKKQVVVKSVDVEEAAMLFDAARLVIVVPGYGMAVAQ
                     AQHAVRELMNVLEKKGCAVKFAIHPVAGRMPGHMNVLLAESNVPYEHMYSLEEINDDF
                     RNADIALIIGANDVVNPAARHDQSSPIYGMPILNADYAKTVIINKRSMAAGYAGIDNE
                     LFGFPNALMYFGDAKDAVSKLTNELKSMNN"
     gene            3363..>3874
                     /locus_tag="PK754_13715"
     CDS             3363..>3874
                     /locus_tag="PK754_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10608.1"
                     /translation="MKRFTIVRYQHVVYYTGLYLVIFFWLGSMPSVVAQRSFIMQNET
                     GVITQDRPSGNSIGVITTTGDTVFIAAGSNLNITTNAGAAWNIIGRSDGLYKGSPVAI
                     THHKSVLYVTTAYDSSLAVEPTYGVSGGISISKDFGSTWSHIAQPTDTGTIRGPVVRT
                     DARTSQPVVL"
BASE COUNT          992 a          964 c          903 g         1015 t
ORIGIN      
        1 aaaaaagacg gcgcgtcaga tggccggacc actcaatgaa gcgatacgta atgtacttag
       61 caaaattgac ggactaagtg aaagacctgt ggtcaattgc gcaacatgcc atcgcggcga
      121 agtcaaacca gctacgcggg taaaataaat caattcaagc catttacagc cgaaatacct
      181 ttcacgaaag gtttttgtca tactataata ttcacattgt gtgatgcata ggccgatttc
      241 ttcaaaaata gtcccctctt gaatactctt tttttttagc gtacaatatg accgaagtca
      301 ttctttttcc cgtctcatga ctcttaatta agtgtgaatt tgcatacacc cccggctatt
      361 aaggagatta ttccgtggtc ataggaattc ccaaagaaac tgcagcaggc gaaaaccgcg
      421 ttgccgtgac gcccgatact gctgccaaac tcatcaaaat ggggtttgaa gtccgcgtcg
      481 aaaaaggcgc cggacaaaat gctcattata cggatgaaca atacaccaaa catggcgcgg
      541 ccattgccgc tacggcgaca gacctgctcg gtcagtcgga tatcattctc aaagttcaaa
      601 aacccagcga tgcggaaatc aaacaaatca aaaaaggcgc attgctgatt tcgtttgtgt
      661 atgggctaaa ttatccgcaa gtgcccaaag cgtgtgctga agcgggcgtc aatctgatgt
      721 ccatggactt cattccgcgc actacactgg ctcaaaaaat ggacgccctg agttcgcaat
      781 ataacatcgc cggctataaa gccgttctga tgtgtgctgc ggctttcggc aaaatgttcc
      841 ctctgatgat gacggccgcc ggtacgattt caccggccaa agttgtgatc atgggcgccg
      901 gcgttgccgg attgcaggct ataggaacgg ctcgccgtct cggtgctgtc gtcgaagtgt
      961 ccgacgtgcg cgctgcggtc aaagaagaag tgcagagtct cggcggtaaa ttcatcgaag
     1021 tgcccggcgc tgaaaatatg caggatgccg gcggatatgc caaagaagcc tcggcagaat
     1081 ttctgcaaaa acaaaaagaa ttacttttca aacacatcac cgaagccgat atcgtcatta
     1141 ccaccgcgct ggtacccggt aaaaaagcac ctgtgctcgt caccgaagaa atgatcaaac
     1201 acatgcgcca cggttccatc gttttggata tggcggtcga atttggcgga aattgcgaag
     1261 tcagcgaagc cggtaaaacc gtaaccaaac acggtgtaac cattatcggc gaatccaata
     1321 ttcccgctac cgtacctacg catgccagcg aattgtattc caaaaacctg ctcgcattgc
     1381 ttcagtacat cgccaaagac ggcaacctcc agctcaaact ggaagatgaa atcgtcaagt
     1441 cggcgttact ggtacacaat ggcgaggtag tcaacgaacg cattaaacaa gttttataac
     1501 cgatcccgga ggatatgtat atgggtgata tcgttatgct catcacggtt tttgtgctcg
     1561 cctgtttcgt gggcgtggaa ttgatttcca aagttccccc gacactgcac acaccgctga
     1621 tgtccggttc caatgctatt tcgggcatta ccatcgtagg ctcgctgatc agtgccggta
     1681 tcggtgaaat gaccatcagc actattatgg gcgtgattgc tgtggctttt gccatgatca
     1741 atgtggtcgg cggttatctt gtcactgacc gtatgctcaa aatgtttaaa agaaaataat
     1801 tttctcagcg ataaaccaac aggttcacta tggaacattt ccgcgaaagt attgaaaata
     1861 tcatgtacct catcggcgcc gtgcttttta ttttcggcat caaaatgctc ggctcgccga
     1921 agacggcacg taaaggtaat ttttattcag cggtcggtat gctgatcgcc atcgttgtta
     1981 cgttatttga caaacacatt cttacgtacg aatggattat cgtaggcatt ttggccggct
     2041 cggtcatcgg tattattttt gccgtcaaag tagaaatgac ctccatgccg cagatggtcg
     2101 gtttgctcaa cggatttggc ggcggtgcat cgacactggt agccttggcg gaatattacc
     2161 gcggtatggc gacctatcaa actgcgctcc agtccaatcc atctttgaca ctgctcgaac
     2221 atatgcccta tcaaaccacc gtttcaatag tactcggcat tcttgtcggc gcggttacat
     2281 tcaacggctc actcgtcgcg tatgccaaac ttcagggttt gatttccggt aatagtatcc
     2341 gttatcctat gcaacatccg ctcaacgcgc tgatgatcgt tgttacgctg gtcatcggcg
     2401 ccttaggtgt tatggacttg aataaccaat ggtacttttt aacggtcact atactcggcc
     2461 ttattctcgg tatcacgctt gtgattccca tcggcggcgc cgatatgccg gtagttatct
     2521 cgttactcaa ttcctactcc ggtattgccg cggcgattac gggatttgtg attaacaata
     2581 atatgttgat catcaccggc gcattggttg gcgcttcggg tatcattctt acggacatta
     2641 tgtgcaaagg catgaatcgt tcactgatga atgtgctcct cggcggctgg ggtacggcgc
     2701 cgactgtcga agcctcgaaa gataaaaaac aagtagtcgt caaatcggtg gatgtcgaag
     2761 aagccgctat gctgtttgat gccgcacgtc ttgtgatcgt cgttccgggt tacggtatgg
     2821 ccgtcgcgca agcgcagcat gctgttcgcg aactgatgaa tgtactcgag aaaaaaggat
     2881 gcgccgttaa attcgctatt cacccggtcg ccggtcgtat gcccggtcac atgaatgtgc
     2941 ttctagcgga atccaacgta ccttacgaac acatgtattc gctcgaggaa atcaatgatg
     3001 atttccgcaa cgccgatatt gctctcatca tcggtgccaa tgacgtcgtc aatccggccg
     3061 cacgccatga ccagtccagc ccgatttatg gtatgcctat tcttaatgcg gattacgcta
     3121 aaaccgtgat tatcaacaaa cgctccatgg cggccggtta tgccggtatt gataacgaac
     3181 tcttcggatt ccccaatgca ttgatgtatt ttggcgacgc caaagatgcc gtttccaaac
     3241 tcaccaacga gctgaagagt atgaataatt agaacccccc ttcgtttgat agacggttac
     3301 acacaatcgc catgatcaca aatgttcatg gcgattgtcc tttttatcct ttatcttttt
     3361 ttatgaagcg ttttactatc gtacgttatc aacacgtagt gtattataca ggcttgtatt
     3421 tggtcatttt cttttggttg ggatcaatgc catccgtcgt tgcgcagcgt tcatttataa
     3481 tgcaaaatga aaccggtgta attacgcaag accgtccttc cggcaattcg atcggtgtta
     3541 ttaccacaac cggtgataca gtttttatcg ccgcaggaag caacctcaat atcaccacaa
     3601 atgcgggtgc agcatggaac atcatcggac gctctgacgg attatacaag ggttcgcctg
     3661 tcgccatcac gcatcataaa tccgttttgt atgtaacgac ggcttacgat tcgtccctcg
     3721 ccgttgagcc aacctacggc gtcagcggcg gtatttccat ttccaaagat ttcggcagca
     3781 cttggtcgca tattgcacaa cccaccgaca ccggcaccat tcgaggaccc gtcgtacgaa
     3841 cggatgcacg tacatcgcaa ccggtcgttc ttaa
//
LOCUS       DAOQNQ010000299         3863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_10954_length_3863_cov_4.275473, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000299 DAOQNQ010000000
VERSION     DAOQNQ010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10954_length_3863_cov_4.275473"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PK754_13720"
     CDS             <1..416
                     /locus_tag="PK754_13720"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNB10609.1"
                     /translation="IPLAPYATPSTTELPDSVRPIIRHADACLLANHGVVTCGRDVYD
                     AYYKLERVEHYARILFVAKSLGGAKILSPQQVHKLETIRATYGTSDAHNPGCIACHGA
                     CIGSDCTLYETQQRDQSASDLHVEETVRAIISAKK"
     gene            417..1208
                     /locus_tag="PK754_13725"
     CDS             417..1208
                     /locus_tag="PK754_13725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNB10610.1"
                     /translation="MNTEFGNRSPLRSLLTIIAAVVFTFLMIIAATIAALFGPRSKAT
                     HAVEKFWARTILRMAGVRARVRGLEHIRPDHVYIFISNHQGLFDIPLMMAYTPKQLRM
                     IHKKELLWVPFFGFILWLLDFIAIDRGNRQRAAESLHRAAKKIHDGTNIVIYADGTRS
                     IDGELRPFKKGAFILAIDAQVDVVPVTISGTINVIHKFEGVFDIRFGQTVELVFSPPI
                     STDGMMPEAKDDLKDQVQNIIAAEYSKIKHLSAITNPAILSRLKK"
     gene            1328..2476
                     /locus_tag="PK754_13730"
     CDS             1328..2476
                     /locus_tag="PK754_13730"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNB10611.1"
                     /translation="MKRIGLFGSTGSIGENVLQVIRNHPDRFTVQCLTANTNAELLIK
                     QALEFKPQSVAIVSDEGYAQLHSGLSGTGIRIYHGHDAIVQMARDENYDLMIGAIVGF
                     AGFLPTIEAIRAGKDIGLANKETLVVGGAIVNGLLSKHQSRLIPIDSEHSALFQCLVG
                     EDMRSVRKLILTASGGPFLHTPAEDFSSITVARALKHPNWSMGAKITIDSATLMNKGL
                     EVIEAHWLFGVGADRIDVAIHPQSIIHSMVEFVDGSTKAQLGVPDMKVPIQYAMTYPE
                     RMTNNFDVFDFRKWNRLDFYEPDRNKFRCLKLAYDALETGGSMPAVLNAANEIAVAGF
                     LQEKIRFTDIASSIESAMQAHTCVCDPDIASVIETDRWARAYVQKTCL"
     gene            2505..>3863
                     /locus_tag="PK754_13735"
     CDS             2505..>3863
                     /locus_tag="PK754_13735"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNB10612.1"
                     /translation="MSIIFDALIPFLIVLGILVFIHELGHHLAAKLFGMKVDAFSLGF
                     PPRAWGFHWATKKLRPKFIKDITQSILSNTTFQSLDKEIQAAGLAQSSDASVKFLDTI
                     FEITQVEEKIELIDAKGKKEEKINLRTELRFAESGPAASLTNLQRDAWLVRVQGDADL
                     LDDLLKYGEINDDKTFHEKYKTDYCLSWVPLGGYCKINGMVDESLDEDSMKEGTPKPW
                     EYRAKPIWQRMIVITGGVFFNFLLAAFVFTLMGLVRGLPDMEKFDNWRRENALPAEVG
                     VVSPGSPADKAGIQAGDRIASINGQTVQKWDELVDVIRHNGGKKLPIEWTRGSERFSG
                     EVMPTIDKIQTEKGIEEIGRIGIQAPPFPEFTRDASFIEAVQHGFITAVGATYFIVRS
                     IKQMVSGEVNAKDALGGPLMIAKTTGDMKRQYGWEGIWNLMALLSLSLAVFNLLPVPA
                     LDG"
BASE COUNT         1039 a          853 c          960 g         1011 t
ORIGIN      
        1 cgataccttt agcgccgtat gccacgcctt cgacgacgga acttccggat tctgtacgtc
       61 cgattattcg ccacgctgat gcatgtttat tagccaatca tggcgttgta acatgcggta
      121 gggatgtgta tgatgcgtat tacaaattag aacgtgtcga gcactatgcg cgtattttgt
      181 ttgtggcgaa aagcctcggc ggtgcaaaaa tattatcgcc gcagcaagtg cataaattag
      241 aaaccattcg tgccacgtat ggtacatccg atgcgcataa ccccggatgc atagcgtgcc
      301 atggcgcttg catcggaagc gattgtacat tatacgaaac ccaacagcgt gatcaatccg
      361 catcggattt gcatgtggaa gaaaccgttc gcgctatcat ttcagccaaa aaataaatga
      421 atactgaatt tggtaatcgc tcacctttac gatcgctgtt gacgataata gcggcggttg
      481 tttttacatt tctcatgatt attgcagcga cgatcgcggc gctgttcgga ccgcgttcta
      541 aagcaacgca tgccgtcgaa aaattttggg cgcgaacgat acttcgtatg gccggtgtac
      601 gcgcgcgggt tcgcggtctg gagcacattc gtccggatca tgtgtatatt tttataagca
      661 accaccaagg cttatttgac attccgctca tgatggctta tacccccaaa cagctccgta
      721 tgatccataa aaaagaattg ttatgggttc ctttttttgg atttatactt tggttgcttg
      781 attttattgc catagaccga gggaaccgtc agcgtgcagc agaaagtctt catcgcgcgg
      841 caaaaaaaat acatgacggg acgaatatcg ttatctacgc cgacggtaca cgcagtatag
      901 atggagagtt acgtccattc aaaaaagggg cattcatatt ggcaatcgac gcacaagtgg
      961 atgtggtgcc tgttacgatc agcggtacga tcaatgttat acataagttt gaaggagtgt
     1021 tcgatattcg ttttgggcaa acggtcgaat tggttttttc gccaccgata agcaccgatg
     1081 gaatgatgcc ggaagccaaa gacgatctga aagatcaagt acagaacatt atcgccgccg
     1141 aatattccaa aatcaaacat ctttctgcga taaccaatcc tgctatacta tcccgcttaa
     1201 aaaaataatt tagctttgtg aaagttgtca cataaacacg cgcaaaggca ggatacgcaa
     1261 agtgtgtttt tcttttgttt gaaatgtatt tttgttatta tggcggcgca caaaactaag
     1321 tgagagtatg aaacgcatcg ggttatttgg ttctacgggc tctatcggcg agaatgtttt
     1381 gcaggttata cgcaatcatc ccgatcgttt taccgtccaa tgtctaaccg caaacaccaa
     1441 cgccgagttg ttgatcaaac aagcgctgga atttaaaccg caatctgtag ctatcgtctc
     1501 cgatgaaggg tacgcccaat tgcatagcgg cctatccggt acaggtatcc gtatttatca
     1561 tggacacgat gccatcgtac aaatggcgcg tgatgaaaat tacgacctca tgatcggcgc
     1621 gattgtagga tttgccggat ttttacctac gattgaggcg atacgtgcgg gtaaagatat
     1681 aggtttagcc aataaagaaa cgctcgtcgt cgggggcgct atcgtcaacg gacttctgag
     1741 caaacaccaa tcgcggctta taccgattga cagcgaacac agcgcacttt ttcagtgcct
     1801 tgtcggtgaa gatatgcgca gcgttagaaa acttattctc acggcatccg gcggcccctt
     1861 tctgcatacc ccggcggagg atttttcatc gatcaccgta gcccgcgctt taaaacatcc
     1921 caattggtcc atgggcgcta aaataacgat agattccgcc acgctgatga ataaagggct
     1981 tgaagtcatc gaggcgcatt ggctttttgg tgtcggggcg gatcgtattg acgtggcgat
     2041 tcatccgcaa tctataattc actctatggt tgaatttgtg gacggctcga cgaaagcgca
     2101 actcggtgta ccggatatga aagttcccat acaatatgcg atgacctatc ccgaacgcat
     2161 gaccaataat tttgacgtat ttgattttcg taaatggaat agacttgatt tctatgaacc
     2221 cgacaggaat aaatttcgtt gtttgaagtt ggcgtatgat gcgcttgaaa caggcggttc
     2281 tatgccggcg gtgctcaacg cggccaacga aatagccgtc gcgggatttt tacaagagaa
     2341 aattcgtttt acggatatag catcgtcgat cgaatcggcc atgcaggctc atacctgcgt
     2401 gtgcgatccg gatatcgcgt cggtgattga aaccgatcgg tgggcgcgtg cgtatgtaca
     2461 aaaaacttgt ttataacaac ttgagtaaaa tataaaggaa taccatgagc attatttttg
     2521 acgcgttaat tccgtttctg attgtcctcg ggattctggt ttttatccac gaactcgggc
     2581 accatctggc ggccaaatta tttggtatga aagtggacgc cttttcgttg ggttttcctc
     2641 caagagcgtg ggggtttcac tgggccacca aaaaactacg ccctaagttc atcaaagata
     2701 tcacacaatc tattttgtcc aataccacat tccaatcatt ggataaagaa atacaagccg
     2761 cgggtttggc gcagtcatcc gacgcatcgg tcaaatttct agatacgatc tttgaaatca
     2821 cacaggtcga agaaaaaatc gaactaatcg acgctaaagg gaaaaaagaa gaaaaaataa
     2881 atctccgcac ggaattgcgg tttgccgaaa gcggtcctgc cgcatcattg accaatctac
     2941 aacgcgatgc atggctggtg cgtgtccagg gtgatgccga tttattggat gatcttctga
     3001 aatatggcga aataaacgac gataaaacgt ttcacgaaaa atataaaacg gactactgtc
     3061 tgagttgggt gccgctcggc ggatattgca agatcaatgg tatggtggat gagagtcttg
     3121 atgaggacag catgaaagag ggtactccta agccatggga atatcgtgca aagccgatct
     3181 ggcaacgtat gattgtcata accggcggcg tgtttttcaa ttttctcctg gcggcttttg
     3241 tctttacgct gatgggactc gtgcgcggtt tgccggatat ggaaaaattt gataactggc
     3301 ggcgcgaaaa tgcgttgccc gcggaagtcg gcgtcgtaag ccccggcagc cctgcagata
     3361 aagcaggcat tcaggccggc gatcgtatcg ccagtattaa cgggcaaacc gtacaaaaat
     3421 gggatgaact ggtggatgtg attcgccata atggcggaaa aaaacttccg atcgaatgga
     3481 cgcgcggttc ggaacgattt agcggcgaag tcatgccgac gattgataaa attcaaactg
     3541 agaaaggtat tgaagaaatc ggacggatcg gaatacaggc gcccccattt cctgagttta
     3601 ctcgcgatgc atcgttcatt gaagccgtac agcatgggtt cataacggcc gtgggcgcga
     3661 cgtattttat cgtgcgttcg atcaaacaaa tggtttccgg cgaggtcaac gccaaagatg
     3721 cgcttggcgg tccgcttatg atcgctaaaa cgaccggcga catgaaacgc caatacggtt
     3781 gggaaggcat ttggaatctg atggctttgc ttagtctcag tcttgctgta tttaatctgt
     3841 tacccgttcc cgcgctcgac ggc
//
LOCUS       DAOQNQ010000300         3856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_10985_length_3856_cov_5.017890, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000300 DAOQNQ010000000
VERSION     DAOQNQ010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10985_length_3856_cov_5.017890"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1779
                     /locus_tag="PK754_13740"
     CDS             <1..1779
                     /locus_tag="PK754_13740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB10613.1"
                     /translation="TGDLDITGGTFAIGSESMSIVGNISNAGTITHTSGTVHFTGNST
                     QTLNGAGTATFNNVTVNGSSNTVAPNANYDVTGNLRVVAGTFAPSATDAYYNVDSLVV
                     HSGGTFDGTNLTATWLSGSFRNHGTLTALADTTVFKATAAGLTVTGGPTFENLRIIDS
                     GRVTLSSNTSVSDELQLVGGFIVTGSNSLILTKTATDPVTGGDSLSFVDGNVVITWPA
                     SQTYVARTFPTGNALYRFFRPIEIAASTDATPPTVRASLTRSPANAVTLNGGLVKVSD
                     IRYHTVTRTAGNDFTGSDLTARLVYARRVLSDYVTAEANLRVARSTDFSTWDNIGGSG
                     VDTLESATTDGDTAGYILSNATSLAGSNSPTYFTLGTTTFDNSLPVELAYFRAMPTDG
                     QIRVEWRTESETENAYWLVQRREVNDEEAEHYRSTGKLSNTNAQYQTVSSIEGQGTKA
                     SATDYNYLDKSAETGKMYAYRISDVGYDGTVTAHNVVVVSMDMPRTFALKSNYPNPFN
                     PTTTIRYELPVSAKVTIKVYNILGQEVTTLVDADQTSGFQKVVWNGLNKYNQQVASGV
                     YIYRMIAVSKDGKQSFTQTKKMMMLK"
     gene            complement(1848..2534)
                     /locus_tag="PK754_13745"
     CDS             complement(1848..2534)
                     /locus_tag="PK754_13745"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB10614.1"
                     /translation="MHRILVIIPAFNAHSTINGILSVLNQFKDRSRPLDIDLDTLVVD
                     DGSDPRLETHLNSGYGHIITHTLNKGKGAALKSGFKFASDEKYDYVVTMDADGQHDPN
                     RLLEMVSFGLRGDYDLVIGERNISLKSMSLGRFLSNKITTKIIRRMSGLDIKDSQCGF
                     RFIKIDLLEKFELHTDNFDTESEFLLRASKYKIQYANFPIPTINNLSHSHINHLRDTV
                     RFIRLLLKSI"
     gene            complement(2585..2658)
                     /locus_tag="PK754_13750"
     tRNA            complement(2585..2658)
                     /locus_tag="PK754_13750"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2622..2624),aa:Arg,seq:acg)
     gene            2806..>3856
                     /locus_tag="PK754_13755"
     CDS             2806..>3856
                     /locus_tag="PK754_13755"
                     /inference="COORDINATES: protein motif:HMM:NF033936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SO_0444 family Cu/Zn efflux transporter"
                     /protein_id="HNB10615.1"
                     /translation="MLQSIFISIADTLVDASFYLLIGFILAGILRGLFTVEMLGKHLG
                     DSSWRSVWKAALIGVPLPLCSCSVVPVGISLKKNGASNGATMSFLVSTPETGVDSIAV
                     SYAFLDPIMTIIRPIAAFITAIVSGSLENIFGSQKQSDTVQQSDAPQTSCNCSTNCNN
                     ETDKILETRFDRLKSGIVYAFTDLLGDVAYYLLLGLIASGILSAIIPENFFIDVMHSP
                     LVNMMIMLAVGIPIYVCASASTPIAAALIMKGITPGAALIFLLAGPATNISTIALVRS
                     KLGQRSLIIYLFSIAMIALAMGSLTDSVYSYFDIKPQVSIGTMHDVIPESIHIISAVI
                     FLPILLLACFREIRHR"
BASE COUNT         1109 a          758 c          856 g         1133 t
ORIGIN      
        1 accggtgatt tggatattac tggtggtaca tttgctattg gaagcgaatc gatgtccatc
       61 gtcggcaaca tttctaatgc cggaacgatc acgcatacgt ccggaactgt tcattttacc
      121 ggtaattcca cacaaacgct gaacggtgcc ggtactgcta catttaacaa tgtaacagtt
      181 aatggaagtt caaatacagt tgcgcccaac gcaaactacg atgtaacagg taacttacgt
      241 gttgtagccg gaacgtttgc accgtccgct acggatgcgt actacaacgt ggacagctta
      301 gttgtacaca gtggtggtac ctttgacgga accaatctaa cggctacatg gttgagcgga
      361 agcttccgta atcacggtac cttgacggct ttggctgata ctacggtatt taaagcaaca
      421 gctgcgggtt tgacggttac cggcggtccc acgtttgaaa accttcgtat cattgatagc
      481 ggtagagtga cattgtcatc gaatacatcg gtttctgatg agctgcaatt agttggtgga
      541 tttattgtaa cgggatcgaa ttcattgata ctaacaaaaa ctgcaactga cccggtgaca
      601 ggcggagatt cattgtcctt cgttgacggt aatgtggtga ttacatggcc cgcctcccaa
      661 acttatgttg ctcgcacatt ccctacgggt aatgcgttat accggttctt ccgacccata
      721 gaaattgcag caagtactga cgcaacacct cctacagtgc gagcttcatt aacacgctca
      781 ccggctaatg ctgtaacttt aaatggtggg ttagttaaag tttctgatat tagataccat
      841 accgttacac gtacagctgg gaatgatttt accggcagtg acttgactgc gcggttagtc
      901 tatgcacgac gtgttttaag tgattatgtg acggctgaag caaatctgcg agtagcacgt
      961 tctactgact ttagtacgtg ggataatatc ggtggctccg gtgtggacac cttagaatcg
     1021 gcaacaaccg atggcgatac agcgggttat atattatcca acgctacttc gcttgcaggg
     1081 tcgaatagtc cgacgtattt taccttaggc acgacgactt ttgacaattc attgccagtt
     1141 gagttggcct atttccgagc aatgccgaca gacggacaaa ttcgtgtcga atggagaact
     1201 gaaagtgaaa cggaaaacgc atactggctt gttcagcgtc gcgaagtaaa tgatgaggaa
     1261 gccgaacatt atcgttcgac cggtaaactt tcgaatacca atgcacagta tcaaactgta
     1321 tcaagtatcg agggtcaagg taccaaagcc tctgcgacag actacaatta tcttgacaag
     1381 tctgccgaaa caggcaaaat gtatgcttat cgtatttccg acgtcggtta tgatggtact
     1441 gtcacagcgc acaacgtagt tgtcgtatcc atggatatgc cgcgtacgtt tgctttgaag
     1501 tctaattatc cgaatccgtt taatccgacg acaacaatac gttacgaatt gcctgtatct
     1561 gctaaagtta ccattaaggt gtataacatc cttggacaag aagttacaac acttgttgat
     1621 gctgaccaaa catcaggatt ccaaaaagtt gtttggaatg gtttgaataa atacaaccaa
     1681 caagtagcaa gcggtgtata tatttaccga atgatagccg tatcgaaaga tggtaaacaa
     1741 tctttcacgc agaccaagaa gatgatgatg ttgaaataag catcttttaa gaagtaataa
     1801 aaaaagccca tcaattgatg ggcttttttg ttttaatata cagaaaacta aatagatttc
     1861 agtaaaagcc gtataaagcg gacggtatca cgcaagtgat tgatatgact gtgggataaa
     1921 ttgttgattg ttggaatcgg aaaattagca tattggatct tatattttga agcgcgtagt
     1981 aaaaattcag attcggtatc aaaattatcc gtatgaagtt caaacttttc cagcaggtca
     2041 attttgataa atctgaaacc gcattgacta tctttaatat ctaaaccgct cattctacga
     2101 ataatttttg tggtaatttt gttagagaga aaacgcccca aactcattga ttttaagctt
     2161 atatttctct cgccaattac caaatcgtag tcgccgcgca acccaaacga aaccatctca
     2221 agcagtcgat tgggatcgtg ctgtccgtcg gcatccatcg ttacgacata gtcatacttt
     2281 tcatcggaag caaacttaaa acctgatttt aaagcagcgc ctttgccttt attgagtgtg
     2341 tgtgtaataa tatgtccgta gcctgaatta aggtgagttt ctagcctggg atctgaaccg
     2401 tcgtctacaa caagcgtgtc taggtcaata tcaagcggtc gggaacgatc tttaaattga
     2461 tttaaaacag ataaaatgcc attgattgtt gaatgggcat taaaagcagg tatgataacg
     2521 agaatgcggt gcatcgaagt gccgtaaagt aaaaaaggct ttagaaaaac taaagccttt
     2581 gttgtgcacc cgtagggagt cgaaccccaa accttctgat ccgtagtcag atgctctatc
     2641 caattgagct acaggtgcca atgacgtcgc aaatatatac ggcgagacat ggaaatgcaa
     2701 gaaaattctt taagtaataa cagtacagtt tatgatgtaa ttgatacagt gaaatatcaa
     2761 tgatggactt ggtttaacgt cagatagagc atatattaca cacatatgtt acaatctatt
     2821 tttatatcaa tagccgatac acttgttgat gcgtcatttt atttacttat tggttttatc
     2881 ctagccggta tattaagagg tctctttaca gttgaaatgc tcggtaagca tcttggggat
     2941 tcttcctgga gatcggtatg gaaagcggcg ctcataggag taccattgcc gttatgttca
     3001 tgcagtgtgg ttccggtcgg aatatcgcta aaaaaaaacg gcgcaagtaa cggtgctaca
     3061 atgagctttt tagtgtccac tccggagaca ggggtggact cgatcgcagt atcgtatgcc
     3121 tttttggatc ctatcatgac gattattaga ccgattgcgg cattcattac tgccatagtc
     3181 agcggttcac ttgaaaatat ttttggttca caaaagcaga gcgacactgt acagcaaagt
     3241 gacgccccgc agacttcgtg taattgttca acaaattgta ataatgaaac tgataaaatt
     3301 ttagagacgc gatttgatcg tttaaaatca ggaattgttt atgcctttac ggatttactg
     3361 ggtgatgtag cttattattt acttcttggg ctgattgcct ctggcatttt atcggcaatc
     3421 ataccagaaa acttttttat tgatgtgatg catagcccgc tggtgaatat gatgataatg
     3481 cttgcggtgg gaattccaat atatgtgtgt gccagtgcgt caacacccat agccgctgct
     3541 ttgatcatga aaggcattac tccaggagcc gcactgattt ttttgttagc agggcctgca
     3601 acgaacattt caacgattgc gttagtgcgt tctaaacttg ggcaaaggtc actaattatt
     3661 tatttgtttt ctattgctat gattgcattg gcaatggggt cattaactga cagtgtatat
     3721 tcctactttg atataaagcc gcaagtatct ataggtacga tgcacgatgt gattccggag
     3781 agtattcata ttattagtgc cgtgatattt ttacctatat tgctgttagc ttgtttccgt
     3841 gagatcagac accgtt
//
LOCUS       DAOQNQ010000301         3836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_11103_length_3836_cov_5.061888, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000301 DAOQNQ010000000
VERSION     DAOQNQ010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11103_length_3836_cov_5.061888"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="PK754_13760"
     CDS             complement(<1..1254)
                     /locus_tag="PK754_13760"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 2-containing transporter"
                     /protein_id="HNB10616.1"
                     /translation="MHTPDSIQPPRWPIAVLRFLLKKEYLEEIEGDLEEHFADDIEQM
                     SVFKARMYFIWNALTLVRPSLLRHIGSFHIQHHPAMFRNYFKISFRSLMRQPFQSFIN
                     IFGLSAAIGICIFTYAFSRWTYSMDQFHTHKNEVYLTTFYANRDGSDQQFGRSPAPLG
                     AVLRKDFPQIKQTCRVEDRNVIIKYGDLVFNERLRFVDPEFLEMLTFPLKWGQTNALR
                     DMNSIILSEEMAVKYFGSENPVGKTMLIKFDGERSGLFTVGGVAEAFPKARTISFHFL
                     VRFENLQSADPSYDVTDWKTMVQATLVRIEHPADVSIVQRNMAAYQNAHNAAASRDWA
                     ITSFVFEPLATLHQRAHRIRDDISRSTEDNYTTIYYLTAITLLMLALACFNYINIAIV
                     SAAKRLKEIGIRKSIGATRSVVSVQF"
     gene            complement(1262..1588)
                     /locus_tag="PK754_13765"
     CDS             complement(1262..1588)
                     /locus_tag="PK754_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNB10617.1"
                     /translation="MKKTKLGEFEELVLLTVIALRKDAYGVEIKRELEERLNETLSVG
                     SIQSALKRMEEKGYLTSAFGEPTQKRGGKRKRIYTATPAAHRVLSELKSIRAGLWNSM
                     PRLVHT"
     assembly_gap    1593..1692
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1816..2082
                     /locus_tag="PK754_13770"
     CDS             1816..2082
                     /locus_tag="PK754_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10618.1"
                     /translation="MPVKSYLAYPMQDQREALAQALKELPNCEIVPSDNYDVFVIVTD
                     TSDDAQEKEIEEKLLKIENIRFLSLVSGYEDRTTTNTNMQGGLA"
     gene            2079..>3836
                     /locus_tag="PK754_13775"
     CDS             2079..>3836
                     /locus_tag="PK754_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNB10619.1"
                     /translation="MNRRELLKHMATVAAMSAAASIFPGIVFSGEQGIERFLPDEKLD
                     WKKSPCRFCGTGCGLLIGIQNGKAVAVKGDPASAVNRGLCCIKGYHSVMALYAKDRLT
                     KPLIRKNGQLVETTMKEALDLIAQKMKECIDKNGKDSVAIYGSGQWTVPDGYVASKFM
                     KGGIGTNNLECNARLCMASAVTGFMTTFGQDEPMGCYEDIDHADVYILWGNNMAEMHP
                     VLFSRMLDNRLRRGAKIIDFATRTTRTSMAADKSILFKPQTDLAVANAIAYELIRTGR
                     INQNFIQKHTTFKKGKTQIGYGLEDKFAFQDEPQGASLEEYKKFLEDYKPEIVEKVSG
                     VSASDIRYLASFYGDANKKVTSFWCMGMNQHTRGTWIQNLVYNIHLLTGKISTPGNSP
                     FSLTGQPSACGTAREVGCFTHRLPHGEVTNEKDRQHAAEIWKVPVEKIPAKPTYHTVE
                     MFRALDRGDIRFMWIQVTNPMVTMPKLKRYRDGAKKEDRFIVVSDVYPTPTTDIADVI
                     LPSAMWIEREGMFGNSERRTQHFEQMLQPPGECIADAWAMIEVARRLGHGALFPWDSK
                     NYVEEIWKESTSFFFKKLRI"
BASE COUNT         1043 a          876 c          861 g          956 t
ORIGIN      
        1 aaactgtacg gagacaacac tacgcgtagc gccgatagat tttcgaatac cgatctcttt
       61 caaacgtttg gcggcggata cgatagctat gttgatatag ttaaaacacg ctaatgccag
      121 catcaataga gtgatcgccg tgagataata gatagtcgtg tagttatctt ccgtactgcg
      181 ggaaatatcg tctcggattc ggtgcgcacg ctgatgaagc gtagccaaag gttcaaaaac
      241 aaatgatgtg atagcccagt cgcgggaagc ggcagcatta tgcgcgtttt gatatgccgc
      301 catgtttcgc tgtactatgg aaacatctgc cggatgttcg atgcgtacca gtgttgcctg
      361 taccatagtt ttccaatccg tcacatcgta tgaaggatca gccgattgga ggttttcaaa
      421 gcgaaccaga aaatgaaaac tgatcgtacg tgctttcgga aaggcttcgg cgacaccacc
      481 gacggtaaaa agtccgctgc gctccccgtc aaacttgatc agcatggttt taccgacggg
      541 gttttccgat ccgaaatatt tgacggccat ttcttcactc agaatgatgc tattcatatc
      601 ccgtagcgca ttagtctgtc cccatttaag cggaaaagtc agcatttcca aaaattccgg
      661 atcgacaaaa cgcagacgtt cattaaacac cagatcaccg tatttgataa ttacatttcg
      721 atcttcgacg cgacaggttt gttttatctg cggaaaatct tttcggagta cggcgccgag
      781 cggagccggc gaacggccaa actgctgatc ggatccgtcc ctattggcat aaaacgtggt
      841 gagatacact tcatttttat gtgtatgaaa ctgatccata ctataggtcc agcgcgaaaa
      901 tgcatacgtg aaaatacaaa tcccgatcgc cgccgaaagc ccgaaaatat tgataaacga
      961 ctgaaacggc tgacgcatca gactacggaa tgagattttg aaataattac gaaacatcgc
     1021 cggatgatgc tggatatgaa acgaaccgat gtgtctgaga agggatggcc tgacaagcgt
     1081 taacgcattc catataaaat acatccgggc tttgaacacc gacatttgtt cgatatcgtc
     1141 ggcaaaatgt tcttccagat cgccttctat ttcttcgagg tattcttttt ttagaagaaa
     1201 tcgcagtaca gcgatcggcc aacgcggcgg ttgaatggaa tcgggtgtat gcatatttac
     1261 ctcacgtatg aacaagtcgg ggcatggaat tccacagccc ggcccgaatg gatttgagtt
     1321 ccgataacac gcgatgtgct gccggagtcg ccgtataaat acgtttacgt ttaccgcctc
     1381 gcttctgcgt aggctcacca aatgcggaag tgagataacc tttctcctcc atccgcttca
     1441 gagcggattg gatcgaaccg acgctcaatg tttcgttcag gcgttcttcc agttctcgtt
     1501 ttatttccac accgtaggcg tccttgcgca atgcaatcac ggtaagcaat accaattctt
     1561 caaattcgcc gagtttcgtt tttttcatga tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nntatgattt atatcatttt tacctcttac ttatttacat actttcgatt
     1741 gttattactc ttccattacc attcatatcg aaattaaaat cgttaagcat acataccata
     1801 taagatagat gtatcatgcc tgttaaaagt tatttagcat atccgatgca agatcaacgc
     1861 gaagcgcttg cccaagcgct gaaagaattg ccaaactgcg aaatagttcc atcggataat
     1921 tacgatgttt ttgtcatcgt aaccgataca tccgatgatg cgcaagaaaa agaaatagaa
     1981 gaaaaattac ttaaaatcga aaacatccgg tttctgagtt tggtttccgg atacgaagac
     2041 cgcaccacta cgaatacgaa tatgcaagga ggcttggcat gaaccgccgg gaactgctga
     2101 agcacatggc tactgtcgct gctatgtccg cagccgcatc gatttttccg ggaattgtat
     2161 tttccggcga acaaggtatc gaacgttttt tgccggatga aaaattggac tggaaaaaat
     2221 cgccttgccg tttttgcgga acgggttgcg ggcttctgat cggtattcaa aacggtaaag
     2281 ccgtcgctgt caaaggcgac ccggccagtg ccgtcaatcg cggattatgc tgcatcaaag
     2341 gttatcactc cgtgatggcg ttgtacgcca aagaccgtct taccaaaccg ctcatccgga
     2401 aaaacgggca gcttgtcgaa actacgatga aagaagcgct cgatctcatc gcacaaaaga
     2461 tgaaggaatg cattgacaaa aacggtaaag attcggtggc catttacggt tccggtcagt
     2521 ggaccgtacc ggatggttat gtcgcctcca aatttatgaa aggcggtatc ggcaccaata
     2581 accttgagtg caacgcacgt ctgtgtatgg ccagcgccgt aaccggtttt atgacgacat
     2641 tcggtcagga tgagccgatg ggttgctatg aagacattga tcatgccgat gtatatatcc
     2701 tctggggtaa caatatggcc gaaatgcatc cggtgctttt ttcccgaatg cttgataacc
     2761 gcctgcgccg cggcgccaaa attattgatt tcgccacacg tactacacgt accagtatgg
     2821 ccgccgataa atcaatctta ttcaaaccgc aaaccgatct tgccgtcgcc aatgccatcg
     2881 cctacgaact gatacgcaca ggccgcatca atcagaattt tattcaaaaa cacacgacgt
     2941 tcaaaaaagg taaaacgcaa ataggatatg gtttagaaga taagtttgca ttccaagatg
     3001 aaccgcaggg tgcttcgttg gaggaataca aaaaatttct tgaagactat aaacctgaaa
     3061 tcgtagaaaa agtttccggg gtttcggcat ccgatattcg ttatctggcg tcattctatg
     3121 gcgatgccaa taaaaaggtt accagttttt ggtgtatggg gatgaatcaa catacacgcg
     3181 gcacatggat tcaaaacctc gtttataaca ttcatttgct gacaggaaaa atttctacgc
     3241 cgggaaacag tcccttctcg ctcaccggcc agcccagcgc ctgcggtacg gcccgtgaag
     3301 tcggttgttt tacacatcgt ttgccgcacg gcgaagtaac caatgaaaaa gaccgtcaac
     3361 acgcggctga aatctggaaa gttcccgttg aaaaaatccc tgctaaacct acatatcaca
     3421 ctgtagaaat gtttcgcgca ctggatcgcg gcgatatccg ttttatgtgg atacaggtta
     3481 ccaatccgat ggtgaccatg cccaaactga aacgttatcg cgacggtgcc aaaaaagaag
     3541 atcgttttat cgtcgtctcc gacgtctatc cgacgccgac caccgacatc gccgatgtga
     3601 ttttgcccag cgccatgtgg atcgaacgcg aaggcatgtt tggtaactcc gaacgacgca
     3661 cgcagcattt cgaacaaatg ctgcaacctc cgggcgaatg cattgcggat gcatgggcga
     3721 tgatcgaagt agctcgtcgg ctcggtcacg gcgcgttgtt tccgtgggat tcgaaaaact
     3781 atgttgaaga aatatggaaa gaatccacga gtttcttctt taaaaaactc cgaatc
//
LOCUS       DAOQNQ010000302         3826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_11152_length_3826_cov_5.950941, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000302 DAOQNQ010000000
VERSION     DAOQNQ010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11152_length_3826_cov_5.950941"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PK754_13780"
     CDS             complement(<1..427)
                     /locus_tag="PK754_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNB10620.1"
                     /translation="MAQIFPKRANKTPILAAAALGFLGMAVTGFFWYYGSPKFTDVGY
                     RPVQPVPYSHKLHAGDLGMDCRYCHVGVETAAAAMVPPTATCMNCHKLVLPQSEKLLP
                     VRESFANNTPIQWVKVHKIPDYAYFNHSIHVNRGVGCESC"
     gene            complement(608..1567)
                     /locus_tag="PK754_13785"
     CDS             complement(608..1567)
                     /locus_tag="PK754_13785"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNB10621.1"
                     /translation="MTITGYYSILAAIRKLWINNCLIPHFEKVRHGMKEILKSYLTLT
                     KPTIMLLVVFTGATALIVEGSFLHEPVKFILVLVGLYLTGGCANGLNQFFERDIDAKM
                     ERTKSRRPLPGGILSPMHALIFCSIIGVSGVILFAVTFNWLTAILSLATILFYSFFYT
                     LYLKPNTSQNIVIGGAAGAMAPVGAWAAATNTMAPAPWIMFLIVFFWTPPHFWALALF
                     CKNDYIKAKLPMMPVVKGDDSTLRQMFYYSLVLVVTTLALIFFKSGWIYAVTAIILGF
                     FFVKKAYDAMKYKTEKIIRGLFGYSIVYLFALFLAMIVDSLVA"
     gene            1878..2399
                     /locus_tag="PK754_13790"
     CDS             1878..2399
                     /locus_tag="PK754_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNB10622.1"
                     /translation="MSLLHFLRAAFFSAVFSSVAFAGDFGELIVRITTKDGSPVAGSR
                     IALAQTPYSAVTNEEGTVRFYLLQSGVYALHLADETDTSAEPVNVYISGDYPTEIDLT
                     LENGKLEIYRGQSNPETMVQKYFSHTVRLKTAQTQPKLRLCKTDVMPRDLFSICRVRF
                     IPTFLTMRLNARS"
     gene            2303..>3826
                     /locus_tag="PK754_13795"
     CDS             2303..>3826
                     /locus_tag="PK754_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10623.1"
                     /translation="MQNGRHAEGFILNMPGAIYTNFSHDAIERSFITKPILPVIDPES
                     QSGVIHIRSDFSDALGYRVNGFPTTDVFDSRHHIQLPHRAIASVLLQEGGSDTRTGNH
                     VRTADIQTKQGGTSWFAAGSIFSDMPAKLLRLPSYGKHVFDGEVSGPIIPQKLYMYSA
                     AEYESFDDADPSVVGVPRYTIAKGSRLYDHDNNNATASIPIDTVLWGNIKNGARPNGS
                     NALRRLNAYARFTAPLDQNLNLEFSGTFSQMKRNWFNMFYLLSPYGVPRSENTAWDIQ
                     SSVHWRVDQQLSFSGGLRYYVNKETITNRKLSSSDSYALRHLQDSYRGRTGFLTYMSD
                     NLFYDVGAGYGMFIRKESSTASVSVSSQYMFSNTLFIESGVSYTSGIYRSATIYDSEN
                     LQFGSNDIFGYSITDDFKLKKKNSGLDGVKKPTLINAYGMGNFKMKNFSGTFGLHVQR
                     LTSGTRELRNLHDPSGLEQQLDENDFYAHEYTTTRFLPRLGVNIQLNEMISAYSHYGW
                     "
BASE COUNT         1107 a          872 c          802 g         1045 t
ORIGIN      
        1 gacaggattc gcagcccacg ccgcgattga cgtgaatgct gtgattgaag tacgcataat
       61 cagggatctt gtgaactttg acccattgaa tcggcgtatt gtttgcaaaa ctctctcgca
      121 caggcaaaag tttttcactt tgcggcagaa caagtttgtg acagttcata cacgtagccg
      181 tcggcggcac catggcggcg gcggcggttt caacacctac gtggcaatac cggcagtcca
      241 tgccgagatc acctgcatgc aatttgtgac tgtacggaac aggttgtaca ggcctgtagc
      301 ctacatcggt aaatttaggc gaaccgtagt accagaaaaa accggtcacg gccataccca
      361 aaaatcccaa agcggcggca gctaaaatcg gagtcttgtt tgcccttttt gggaatatct
      421 gagccactga ggtctcctca ttaatgaata attaaacggt ggataatctt aaaaacttct
      481 aacagtcaaa ctcgggggac atcaattaag gttcacggga cagcaattta gacggacatg
      541 tctaaacttg caagccaatt ttacaaattc tttacaaatg caaagaatta cctagattgt
      601 tcgtcactta agcgacgaga ctgtccacta tcatggccaa aaaaagcgca aaaagataca
      661 caatggagta accaaaaaga ccgcgaatga ttttttcagt tttatatttc atagcgtcgt
      721 acgctttttt tacgaagaag aaaccgagga taatcgccgt aacggcataa atccaaccgg
      781 atttgaagaa aataagagcc agcgtggtaa ctaccagcac gagagaataa taaaacattt
      841 gacggagtgt actgtcatca cctttgacga caggcatcat cggtaatttg gctttgatat
      901 aatcgttttt acaaaacagc gccaacgccc aaaaatgcgg tggcgtccaa aaaaacacga
      961 tcagaaacat gatccacggc gccggcgcca ttgtattggt cgcagcggcc caggcaccga
     1021 ccggggccat cgcccctgcc gcaccgccga taacgatatt ctgtgacgta ttgggcttga
     1081 gataaagggt atagaagaaa ctgtaaaata aaatagttgc taaagataag atggccgtaa
     1141 gccagttgaa agtcacggcg aaaagaatca cgccggagac accgataatc gagcaaaaaa
     1201 tcagcgcgtg catcggactc aggattccgc ccggcaaagg acgacggctt ttggtgcgct
     1261 ccattttggc atcaatatcg cgttcgaaaa actgattcaa tccgttggca caaccgccgg
     1321 taagatataa acccaccagt acgagaataa atttcaccgg ctcatgtaag aaactgcctt
     1381 caacgattaa tgccgtcgcg cccgtaaaaa caaccagcaa cataatcgtc ggtttggtca
     1441 gtgtcagata agatttcagg atttctttca tcccgtggcg aactttctca aaatgtggaa
     1501 ttaaacagtt attaatccat aatttccgaa tggcggctaa gatagaatag tagcctgtga
     1561 ttgtcaaaga atatttataa aaaaataatt tcttttttta actcttacct tcagtctatt
     1621 tcgcaacatc tccaatcctg aaaagagttt atacatatct cccagactcc cctctataat
     1681 aaccttttga ctaacctttt gggttggatt gatattacgc acgtattgta tttgcgatgt
     1741 agttatttga taacacatgt cgttagtttt ttcaaattga tccgacttta ctcttgtttt
     1801 taaatcatgc ctaatttaac cttaccatgc ttttatcaaa tctcattctg tttttgactt
     1861 tatctgaggg aaaatccatg tcgttattac attttcttcg cgctgcattt ttcagtgccg
     1921 ttttttcatc ggttgctttc gccggggatt ttggggaatt gatcgtacgt atcaccacaa
     1981 aagacggatc tccggtagcc ggctcgcgca ttgctttagc acaaacaccg tatagcgccg
     2041 tcaccaacga agaaggaacg gtacgttttt atcttttgca gtccggtgta tacgcgttac
     2101 acttagccga cgaaacggat acatcggctg aacccgtgaa tgtatatatt tcaggtgact
     2161 acccgacaga gatagattta actctcgaaa acggaaaact tgaaatctac cggggacaat
     2221 ctaatcctga aacgatggta caaaagtact tttcacatac agtgcgccta aaaacagccc
     2281 aaactcagcc caaactcaga ctatgcaaaa cggacgtcat gccgagggat ttattctcaa
     2341 tatgccgggt gcgatttata ccaacttttc tcacgatgcg attgaacgct cgttcataac
     2401 caaacctata cttcctgtaa ttgatccgga atctcagtcc ggtgttatcc atattcgttc
     2461 cgattttagc gatgcgctcg gttatcgggt caacggattc ccaactaccg atgttttcga
     2521 cagccgtcat catatacagc ttcctcaccg cgccattgct tccgttttat tgcaagaggg
     2581 gggttccgat acgcgcactg gcaatcacgt gcgtacggca gacattcaga ccaaacaagg
     2641 cggtacctct tggtttgccg ctggttctat atttagcgat atgcccgcca aattattgcg
     2701 cttaccttcg tatggaaaac atgtttttga tggcgaagtg tccggtccga ttattcctca
     2761 gaagttatac atgtactcag cggctgagta cgaatcattt gatgatgccg atcccagcgt
     2821 agtcggagta cctcgttata cgatagccaa aggcagccgg ctgtacgatc acgataataa
     2881 taatgccaca gcatctatac cgattgatac cgtgctttgg ggaaatatta aaaacggcgc
     2941 tcggcctaac ggttccaatg cgttgcgccg cctcaatgct tacgctcgct ttacggcgcc
     3001 tttggatcag aatttaaacc tggagttttc aggcacattt tcgcaaatga aacggaattg
     3061 gtttaacatg ttttatcttt tatcgccgta cggtgttccc cgatcggaaa atacggcttg
     3121 ggatatccaa tcatccgttc actggagggt agatcagcaa ttatccttca gcgggggcct
     3181 tcgttactat gtaaataaag aaacgataac caatagaaaa ctctcctctt cagactctta
     3241 tgcgttacgt catttacaag attcgtacag aggacgcacc ggttttttaa catatatgag
     3301 tgacaattta ttttatgatg tcggcgccgg ttatggtatg tttattcgta aggaatcatc
     3361 caccgcatcc gtttccgttt catcgcagta tatgtttagc aatacgctat ttatcgaatc
     3421 cggcgtttct tatacttcag ggatctatcg atctgcaaca atatatgaca gcgaaaatct
     3481 tcaattcggc agtaacgata ttttcggata tagcattacc gatgatttca aacttaaaaa
     3541 gaaaaactcg ggattagatg gcgtcaaaaa gccaacactg atcaatgcgt acggtatggg
     3601 aaattttaaa atgaaaaatt tctcgggaac attcggtctg catgtccagc gcctaacctc
     3661 aggcacacga gagcttcgta atcttcatga tccttccggt ctcgagcaac agctagacga
     3721 aaatgatttc tacgcgcatg aatatactac aacacgtttt ttaccccgat taggagtaaa
     3781 tatacaactt aatgaaatga tatcagctta cagtcattat ggatgg
//
LOCUS       DAOQNQ010000303         3822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_11172_length_3822_cov_5.496416, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000303 DAOQNQ010000000
VERSION     DAOQNQ010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11172_length_3822_cov_5.496416"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PK754_13800"
     CDS             complement(<1..289)
                     /locus_tag="PK754_13800"
                     /inference="COORDINATES: protein motif:HMM:NF016450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10624.1"
                     /translation="MSTKKVIERVNFAKNPSVVPMPDLLEIQIDSYKEFLQEDVPAEK
                     RTNTGLQNVFLSNFPVDDAKELFKLEFLKYYVERPKYDVRECQERGVSFSVP"
     gene            complement(534..923)
                     /gene="rplL"
                     /locus_tag="PK754_13805"
     CDS             complement(534..923)
                     /gene="rplL"
                     /locus_tag="PK754_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNB10625.1"
                     /translation="MAISRADVLEYLASANMMEIGELVKEIKSKFGVEIAAPVAVAAA
                     PGGAPGAGAAAAAEEKTEFTVKLTNAGDKKIQVIKVLREITNLGLKEAKDAVDATPAV
                     VKEGVSKDEAEKIKAKFAEVGATVEVA"
     gene            complement(1017..1544)
                     /gene="rplJ"
                     /locus_tag="PK754_13810"
     CDS             complement(1017..1544)
                     /gene="rplJ"
                     /locus_tag="PK754_13810"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNB10626.1"
                     /translation="MKKENKEQIIADLADKFQKASSLYLTDFTGINVIKMEQLRNKFR
                     ETNSEFKVVKNTLAKQALNKAGYTVDLSKYLQGPTGIAFGYDDAVAPAKVISEFLKEK
                     ENDNLKVKICIIEKEMYAASKIGDIAKMPNKKDMLAQLVGLFNSPMQNVVMVLNAPMQ
                     NLVGVLESLKNQKAA"
     gene            complement(1579..2283)
                     /gene="rplA"
                     /locus_tag="PK754_13815"
     CDS             complement(1579..2283)
                     /gene="rplA"
                     /locus_tag="PK754_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNB10627.1"
                     /translation="MKKHSKRFKAAVAKIEKGKEYSISDTFKMIKETATTKFTETVDV
                     AVNLGVDPKHADQALRGTVMLPHGNGRTVRVLVITKTKEKEAKEAGADLVGFDEYLQK
                     IKEGWTDVDVIVATPEAMIELGKLGKVLGPKGLMPNPKSGTVTPDVTKAVKEVKAGRV
                     EFRVDKQGNVHVGIGKSSFDVKQLEENFKSFMTTIIRMKPATAKGTYLKSVHVSTSMG
                     PSVKVSNTEVGGLTIH"
     gene            complement(2375..2797)
                     /gene="rplK"
                     /locus_tag="PK754_13820"
     CDS             complement(2375..2797)
                     /gene="rplK"
                     /locus_tag="PK754_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNB10628.1"
                     /translation="MKKVVAQIKLQISAGAANPQPPIGPALGQHGVNIMEFCKAFNAK
                     TQDQKGMIIPVIITVYSDRSFTFILKTPPAAILIKKAINLEKGSAQPNRNKVGKISRA
                     KLMEIAKIKLPDLNTKDIKSATSMMEGTAKSLGLDIID"
     gene            complement(2823..3422)
                     /gene="nusG"
                     /locus_tag="PK754_13825"
     CDS             complement(2823..3422)
                     /gene="nusG"
                     /locus_tag="PK754_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNB10629.1"
                     /translation="MENTEKTDSPEIVTKDAPRPENTRWYTLRVYSGQEAKVRDHIKS
                     ELEIAGLKDLVFRIMIPEETIVEMKNGKKKEKTRSFLPGYMIVEMVDDKRVQHVITNT
                     PGVVSFVGPKNMPQPLRPDEVDRMLGRMHEKQTTETIEIPYKVGDAVKIIDGPFADFN
                     GFIDEINYEKKKLKVMVSIFGRSTPVELDFLQVKTVTNS"
     gene            complement(3447..3629)
                     /gene="secE"
                     /locus_tag="PK754_13830"
     CDS             complement(3447..3629)
                     /gene="secE"
                     /locus_tag="PK754_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNB10630.1"
                     /translation="MNRIKEFFMSVQTEMKKVSWPTREELMGSTGVVMVLWLILSLYI
                     FTSDNVLQAIVKKLLL"
     gene            complement(3659..3731)
                     /locus_tag="PK754_13835"
     tRNA            complement(3659..3731)
                     /locus_tag="PK754_13835"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3696..3698),aa:Trp,seq:cca)
BASE COUNT          956 a          899 c          769 g         1198 t
ORIGIN      
        1 aaggaacaga aaacgaaaca ccgcgttcct ggcattcgcg tacgtcatat ttaggacgtt
       61 cgacgtaata cttcagaaat tcaagtttga acaattcttt agcatcgtct accggaaaat
      121 tactcaaaaa tacattctga agaccggtat tggtccgttt ttctgcagga acatcttcct
      181 gcaaaaattc tttataggaa tcaatctgga tttccagaag atccggcatg ggaactacgc
      241 tcggattttt ggcgaagttc acacgctcaa taactttttt tgtactcaat ggaatcctgc
      301 cttttaatta aggttgtcag aaaactctta atttttttgt agaaaaagcg aaaggcatgt
      361 tcgcttgcgg gagaaaccca tattatcgcc tggatttctt tatcggatca aaactaaaaa
      421 acacgccgag agcgcaaaat cgtttttaac gttctccgcg tatttttcaa aaattcaaaa
      481 aacattggaa ggcagttgga ttccaactgc cttccttatc aatccaaaaa cgattaagca
      541 acttcgacgg tagcgccgac ttcagcaaat ttcgctttga ttttttcggc ttcatctttc
      601 gatacgcctt ctttgacaac ggcgggcgtt gcatcaacag cgtctttagc ttctttgagg
      661 ccgagattgg tgatttcgcg gaggactttg atcacctgga tttttttgtc accggcgttg
      721 gtgagtttca ctgtaaattc ggtcttttct tcagcagcag cggctgcacc ggcacccgga
      781 gcgccgcccg gagctgctgc tactgcaaca ggggctgcaa tttcgacacc gaacttagac
      841 ttgatttctt tgaccagttc gccgatttcc atcatgtttg cactggcaag atattccagt
      901 acatctgcgc gagagatagc cattttagga ctcctgactt ttgttgcaac cggttatccg
      961 caacacttac acggacaaca tattaaggcc gcaaacgtta atgaatgaat gaaaaattaa
     1021 gctgcttttt ggtttttcag ggactctagc acacccacga gattttgcat cggggcattg
     1081 agtaccatca ccacattctg catcggcgaa ttgaaaaggc cgacaagctg agccagcatg
     1141 tctttcttat taggcatttt cgcaatgtcg ccgatcttcg atgcggcata catttctttt
     1201 tcgataatac agattttaac tttgagatta tcgttttctt tttctttgag aaactccgat
     1261 ataactttcg ccggagcaac cgcatcatcg taaccaaatg caatacccgt cggtccttgc
     1321 aaatacttgc tcagatcaac cgtatagccg gctttattga gcgcttgttt ggctaatgtg
     1381 tttttgacaa ctttaaattc cgaattggtt tcacgaaatt tattacggag ctgttccatt
     1441 ttgatcacat tgatacccgt aaaatccgtc agatagaggc tggaggcctt ctggaattta
     1501 tcagcaaggt ccgctatgat ctgttcttta ttttcttttt tcatggtcgt agatgtttag
     1561 tgtttgacaa cgcgttaatt agtgtatcgt cagcccgccc acttccgtgt tgctgacttt
     1621 gaccgaaggt cccatactcg tggaaacatg cacacttttc aggtatgtac ctttcgccgt
     1681 agccggcttc atacgaataa tcgtagtcat aaagctcttg aaattttctt caagctgttt
     1741 gacatcaaac gacgacttgc cgataccaac gtgtacatta ccttgcttat caacgcgaaa
     1801 ttccacacga ccggctttca cttcttttac cgccttggta acatccggtg tgacagttcc
     1861 gcttttcgga ttaggcatga ggcccttagg accaaggact ttacctaatt tacccaactc
     1921 aatcatagct tcgggtgtag ctacgatcac atctacatcc gtccaaccct ctttgatttt
     1981 ttgcagatat tcatcaaaac caaccaaatc ggcgccggct tctttggctt ctttttcttt
     2041 ggttttggtg atcacaagga cacgcaccgt acggccatta ccatgcggta acataaccgt
     2101 tccacgaagc gcttgatcgg cgtgtttcgg atcaacaccg agattgacag ccacatcaac
     2161 cgtttctgta aatttagttg tggcggtttc tttgatcatt ttgaaagtat cggaaatgga
     2221 atattcctta cctttctcga tctttgcgac agccgcttta aagcgtttgc tgtgtttttt
     2281 catagttctc cttttgcgaa ttttctgtgc atacggcaca tactggttct cccacatgcc
     2341 tcacggacgt ggtgtgttat gagtaaatta gaaattaatc aataatatcc agacccaagc
     2401 tctttgcggt accttccatc atgctcgtag ccgacttgat atctttagta ttgaggtcgg
     2461 gcaatttgat tttagctatc tccatcagct tagcgcgaga aattttgcct actttgttac
     2521 gattcggttg agctgaacct ttttccaaat tgatcgcttt tttgatcaaa atggctgccg
     2581 gaggtgtttt gaggataaat gtaaacgaac gatccgaata gaccgttatg atcaccggaa
     2641 tgatcatacc cttctgatcc tgggtcttgg cgttaaacgc cttacaaaat tccatgatgt
     2701 tcacgccgtg ctgaccaaga gccggaccga tcggcggttg cggattggct gcgccggcag
     2761 aaatctgcaa cttaatttga gcgacaactt ttttcacagt tgattctccg ttgtgtgttt
     2821 aattatgaat tcgttacggt ttttacctgg agaaaatcca gttccaccgg tgtcgaacgg
     2881 ccaaaaatac tgaccatgac tttgagtttt ttcttttcgt agttgatttc atcaataaaa
     2941 ccgttgaaat ccgcaaaagg gccatcaatg attttgaccg catcacccac tttataaggt
     3001 atttcgatcg tttcggtggt ttgtttttca tgcatgcgac cgagcatacg atcaacttca
     3061 tccggacgaa gcggttgcgg catattctta ggtcctacaa aactcacaac gccgggtgtg
     3121 ttggtgatta catgttgcac acgtttatcg tccaccattt caacaatcat atatcccggt
     3181 aaaaaggaac gtgttttttc tttcttttta ccgttcttca tttcgacgat ggtttcttcg
     3241 ggaatcataa tgcgaaaaac gagatcttta agaccggcta tctcgagttc acttttgata
     3301 tgatcgcgaa ctttggcctc ctgacctgag tacacacgta acgtatacca acgtgtattt
     3361 tcaggacgcg gtgcatcttt cgtcacaatt tcaggagaat ccgttttttc cgtgttttcc
     3421 acttgcgatt cctttaataa atccgattac agaaggagtt tctttacgat cgcttgcagc
     3481 acattgtccg atgtaaaaat atacaacgac aatatgagcc aaaggaccat aacaactccc
     3541 gtagaaccca tcagctcttc acgtgtaggc caagacactt ttttcatttc ggtctgcacg
     3601 ctcataaaaa attcttttat tctgttcatt tgttccttaa ctcctgaata ttttttcgca
     3661 ggggagacag gacttgaacc tgcaacctac ggttttggag accgccgctc tgccaattga
     3721 gccactcccc taaacaagcc cctccgactc ttcgccggag ggatattttt tcttcttact
     3781 tgaggatctt ggttactaca ccggcgccta cggtacgacc ac
//
LOCUS       DAOQNQ010000304         3795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_11303_length_3795_cov_6.421925, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000304 DAOQNQ010000000
VERSION     DAOQNQ010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11303_length_3795_cov_6.421925"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PK754_13840"
     CDS             complement(<1..435)
                     /locus_tag="PK754_13840"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNB10631.1"
                     /translation="MQSILVTGGAGFIGSNFVRYMLKKYPSYRIIVLDALTYAGNRAN
                     LTDLEKNPRFFFYHGNICDRSVVDNLVSNVDAVINFAAETHVDRSIHEADSFAQTDVL
                     GTMVLLEAAKKYNIARYLQISTDEVYGSIENGEFTEEHSLSPN"
     gene            complement(516..1436)
                     /locus_tag="PK754_13845"
     CDS             complement(516..1436)
                     /locus_tag="PK754_13845"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNB10632.1"
                     /translation="MELKSRHLLGLKGMSAEEIELILETALSFKEVLNRPVKKVPTLQ
                     GFTIANLFFEPSTRTRISFELAQKRLSADTVNFTPSGSSTSKGETLKDTARNIEAMKV
                     DMVVIRHSAAGAAWFLSQRLQTNVINAGDGAHEHPTQALLDILTLREKYGSLKGLRVT
                     IVGDITHSRVAKSNIYALKTLGAEVAVCGPPTFIPRNMADLGVRIYDNIHDAIRHSDV
                     LNVLRIQLERQSSGLFPSIREYHARFGINKEKLALGSKHMTIMHPGPINRGVELDSEV
                     ADGEYSVILDQVLNGVATRMAVMYLLSVTK"
     gene            1742..2941
                     /locus_tag="PK754_13850"
     CDS             1742..2941
                     /locus_tag="PK754_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10633.1"
                     /translation="MKSDKGNLSRQDAHLYLENLSKKIALNGNAGSLSKSLAVTGAPF
                     LAKIAKQGHVAINEVTDVSSVITVENQLHEIRMEVFNPQKAQAMSKSAAVTSGDLYGV
                     YADPIDYYDGSSDHVNFPVQIVSLNNGEYSSREEIWTAPYSADMDQIDDEFNQYISAL
                     RENTPYPLYALTIENIEEDPQDGNLNKSAAAGPYLGFEGVYLKVERDGLTDEEFELWW
                     TEPALSGLIHKRDMFNGHHHTDESGANVYFPDVNHKGNWEQLGDEFTTEIRLISLADG
                     IVKALTPWEDDATAGSMKRNSRSSMYNPVIDGVQTGAWADVKVYDDAFNCETNMVDDN
                     VGLHYYVDGFPESKDEDDPYSEGAVYRISAASIANRAPNGVRFETNQALNHKLDDINY
                     RFNYYQD"
     gene            3007..>3795
                     /locus_tag="PK754_13855"
     CDS             3007..>3795
                     /locus_tag="PK754_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10634.1"
                     /translation="MKKIIICLFFPLAMSCQSCYKEYYTDSRLQEEKDIVLSVLAPDT
                     SNGFIRENAIEVFVGKIVPTDIHNIYTVYGMQDSLLKELMINTRQFYIAYYKYDPNAI
                     VSIEGNGQNVILEYTDRGIYRDINRSLKIKPSARYKLKVEKKDGRVFTSETVVLNTLS
                     IQNDFSDTVLVKPYLEAGLFYADTVLLKSSFLGKAIYYVFQEKSNNFSFSVSTYTFQN
                     NAYDAVIFTKNEFDMTRIDFVTVNYETRAIDSAYGMFHQPHGHSM"
BASE COUNT         1117 a          780 c          850 g         1048 t
ORIGIN      
        1 attgggtgaa agcgaatgtt cttcggtgaa ttcgccgttt tcaattgaac cataaacttc
       61 gtcagtggaa atctgcaggt accgcgcgat gttgtatttt ttggccgcct caagcaatac
      121 catagtgccc aaaacatccg tttgtgcaaa agaatccgct tcatggatag aacgatccac
      181 atgagtttcc gcggcgaaat tgattacggc gtccacattg gatacaagat tatctacgac
      241 gcttcggtcg cagatatttc cgtgataaaa aaagaaacgc ggattttttt caagatcggt
      301 aagattggcg cgattaccgg catacgtaag cgcatcgagc acgatgatac ggtacgacgg
      361 gtacttttta agcatgtagc gaacaaaatt acttccgata aatccggcgc cgccggtgac
      421 gagaatggat tgcatacttt tttatatcct aacaaggcaa ttatgtttgc acaaactatt
      481 gtacacagat agtgcaagct tggtcattaa taaatctatt tggtaacgct caaaagatac
      541 attacggcca tgcgtgtggc gacgccattg agaacctgat ccaagatgac ggaatattcg
      601 ccgtcggcca cttcgctgtc aagttctacg ccgcgattga taggtcccgg atgcatgatc
      661 gtcatatgtt tcgaaccgag cgctaatttt tctttgttaa taccaaatcg cgcatggtat
      721 tcacgaatgg acggaaagag tccggaagac tgacgttcaa gctggatacg cagcacattg
      781 agcacatccg aatggcgaat cgcgtcgtgt atattatcat atatacgaac acccaagtcc
      841 gccatgttac gcgggataaa cgtcggcggc ccgcagacgg caacttccgc gcccaatgtt
      901 ttgagcgcat agatgttgga tttggccacg cggctgtggg tgatatcgcc cacgatggtt
      961 acgcgcaaac ctttgagaga tccgtatttt tcacgcagag tcaatatatc taaaagcgct
     1021 tgtgtcggat gttcgtgcgc tccatcgccg gcattgatga cattggtttg taaacgttgc
     1081 gataaaaacc aagccgcgcc cgcagccgaa tggcgaatca ccaccatatc taccttcata
     1141 gcctcgatat tgcgcgcggt atctttgagc gtttcgcctt tggatgtact gctgcccgat
     1201 ggtgtaaaat tgaccgtgtc ggctgacaat cttttttgag ctaattcaaa cgagatgcgc
     1261 gtccgggtgg acggttcaaa aaacaaatta gcaatggtaa aaccctgaag ggtgggaact
     1321 ttttttacag ggcgattgag aacttcttta aacgacagag ccgtctcgag tatcagctcg
     1381 atctcttcag cgctcattcc tttgaggccg agcaagtgac ggcttttaag ctccatagaa
     1441 ggcacacaaa atctcggaaa tttcgcggca atataggcgg gaaggctgtt tttagcaagg
     1501 caattctatt ttggtttggc aaagaatgtc aaaaaaatct tgattttcaa tatgatatca
     1561 tatatgatgt gccatcgttg tttataaaaa aactctaagt attactaagt ctctcggcag
     1621 tcagatatta actaatgagg taatctatga atcagcatcg taaaatgtgg gggggggggt
     1681 agcgcttatt cttgcgttgt tttttacttc ctgcgaaaat tccaatcaaa ccgcatctga
     1741 agtgaaatcc gacaaaggaa atctatcccg tcaggacgca catctctatc ttgaaaattt
     1801 atctaaaaaa attgccttga atggcaatgc aggctctcta tctaagtctt tagccgtaac
     1861 gggagcgcca tttcttgcca agatagctaa gcaaggccac gtggcaatca atgaagtgac
     1921 cgatgtctct tcggttataa cagtagaaaa tcagttacac gaaattcgaa tggaagtatt
     1981 caatccgcaa aaggcgcaag ctatgtccaa aagcgcggcg gtaacttccg gcgatttata
     2041 tggcgtgtat gcagatccga ttgattacta tgacggctcc tcagatcatg tcaatttccc
     2101 tgttcagatc gtatctctca ataacggaga gtattcaagc cgtgaagaaa tatggactgc
     2161 gccttattcc gcggatatgg atcaaattga tgacgagttt aatcaatata tcagtgcgct
     2221 acgcgagaat acgccgtatc ctttgtacgc attgacaata gaaaatatcg aagaagaccc
     2281 gcaagacgga aatttaaata aaagcgcggc ggccggcccg tatcttggat ttgaaggagt
     2341 atatcttaaa gtagaacggg atgggttgac cgatgaagag ttcgaactgt ggtggactga
     2401 gccggccttg tcgggtctca tccacaaacg cgatatgttc aacggtcatc atcacacgga
     2461 tgaaagcggg gccaatgttt attttccgga tgtcaatcac aaaggaaact gggaacaact
     2521 cggtgatgag tttactactg agatccgact gatcagccta gccgacggca ttgtcaaagc
     2581 tttaactccg tgggaagatg atgctacagc cggtagtatg aaacgtaata gccgtagctc
     2641 tatgtataac ccggtaattg atggcgtaca aaccggcgca tgggccgatg ttaaagttta
     2701 cgacgacgcg ttcaattgcg aaactaatat ggtggatgac aatgtcggat tacattatta
     2761 tgttgatggc tttcccgaat caaaagacga agacgatccg tattccgaag gcgctgtata
     2821 tcgtatttct gctgcaagca tagctaatcg ggcgcccaat ggggtaagat ttgaaacaaa
     2881 tcaagcttta aatcataagc ttgatgatat caattatcgt ttcaattatt atcaagatta
     2941 atattttcga attttaaatc aatcagatgc cgagagactg atttatttaa aaccaaatag
     3001 acatatatga aaaaaataat catttgtttg ttttttcctt tggcgatgtc ctgccaatcg
     3061 tgctataaag aatactatac agactcccgt cttcaggaag aaaaggatat tgtattgagc
     3121 gttttagctc cggacacatc gaatgggttc attcgggaaa atgccatcga agtttttgtg
     3181 ggtaagatcg tacctacgga tattcataat atatatactg tatatggcat gcaggattcg
     3241 ctattaaaag aattgatgat caatacccgg cagttttaca tcgcttatta caaatacgat
     3301 cccaacgcca tcgtctccat cgaaggtaac ggtcagaatg tgattttgga atatacggat
     3361 cgcggaattt acagagatat aaatcgtagc ctcaaaatca aaccatctgc acggtacaaa
     3421 ctaaaagttg aaaaaaagga cggtcgggtc tttacatctg aaacagtagt tctaaatacc
     3481 ttatctattc agaatgattt ttcagatact gtattggtca aaccttactt agaagcaggt
     3541 ttgttttatg ccgacactgt gttgcttaaa tcaagttttc tgggtaaagc aatttattat
     3601 gtgtttcaag agaaatccaa taatttttca ttttcggtaa gcacttatac attccaaaac
     3661 aatgcttatg acgctgtaat attcactaaa aatgaatttg atatgacccg aatagacttt
     3721 gttacagtaa attatgaaac ccgcgccata gacagtgcat acggcatgtt tcaccagcct
     3781 cacgggcata gtatg
//
LOCUS       DAOQNQ010000305         3773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_11426_length_3773_cov_3.339430, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000305 DAOQNQ010000000
VERSION     DAOQNQ010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11426_length_3773_cov_3.339430"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PK754_13860"
     CDS             complement(<1..222)
                     /locus_tag="PK754_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10635.1"
                     /translation="MNFYARVTVFLLSLVSILLTACSGKSYSTDTPENCHNAVVTAIR
                     THDFGVLYTLIDTAIRSDVRTYFHNTRSVL"
     gene            311..1393
                     /gene="rlmN"
                     /locus_tag="PK754_13865"
     CDS             311..1393
                     /gene="rlmN"
                     /locus_tag="PK754_13865"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNB10636.1"
                     /translation="MKNIAKNQNLKPETINIFGKTLSELESFFEALGEKKFRAVQVFQ
                     WMYKRGVTNFDDMSDLSKSLRDKLHASAVIITDTVIKIQTSGEKENDVTRKILFQLAD
                     GEKVEAVYIPDEGRKTVCISSQVGCAVACQFCATGWMGFRRNLSVGEIIGQLMYIRRE
                     IDPEISNIVFMGMGEPFLNYENVMNAAGIIISEHGPGLAPKRITISTSGIIPKIYEFA
                     DADLPYNLAISVNATTDEVRQKVMPITKKYPLKDLLESIQYLNARRKNPATLEYVLLA
                     GVNDSDDDAKRLRTMAKQLGKCKVNVIPYNPIEGPDLKRPSPEHIARFMKLVSGISSP
                     LTLRRSRGRDIAAACGQLAIKEKHDV"
     gene            1383..3374
                     /locus_tag="PK754_13870"
     CDS             1383..3374
                     /locus_tag="PK754_13870"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNB10637.1"
                     /translation="MMYKTKRTIVAILTYLIFSVIPARAQTNVVKLSTREAVENGQPV
                     LEIVADIDSKYHVYGMEKLDGPIATEFKFTLPEGIELVGEVMSPEGKRHHDEGFGIDV
                     TWHSGKTVFKQKLKLSATAKTGDIITVAMTYQACTESYCLPPKTIRATHTISQQWIGL
                     SAGTIKPINKINPNQAVGSISLQSHSIAEPENKSSASHDSVAQTVPDKVSTIQERTAS
                     YESLRKESLGSFIGLAFLTGLLALLTPCVFPMIPITVSYFTKHSASSRAGAVRQSVIY
                     VVGIIASFTLFGWLMSLVVGATGAQNFAANAWVNLAIGILFIVFAMSLFGVFEIRLPS
                     FIVNFSQQQANTGQTIGTLMAGVTFTLVSFTCTVQFLGLLMVAAADGEWFLPVIGMLC
                     FATAFSTPFFFLSLFPQYLANLPKSGGWLHATKIVMGLVEIAAAFKFFSNVDLVWDLQ
                     WLTRPAMLSIWITLFGVSGIYLLGKIRWSVEDEVEEIGFGRTMTGLLFISFTVYLLSG
                     LFGNPLQADMDAYLPPSDYGVVRTAGNAKSTESHETWIEDYEAALREARTSGKPLFID
                     FTGKTCTNCRLMEKTMFTRADVQFYFDRMVLTRLWTDFGENMERYQDLQQRLVGSVAL
                     PFYVIITPDEKVLARFGGLERDPEIFKSFLADGLQKSQK"
     gene            3392..>3773
                     /locus_tag="PK754_13875"
     CDS             3392..>3773
                     /locus_tag="PK754_13875"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNB10638.1"
                     /translation="MRWSLAIFVLAGLMSSGCNKPGSGGAVDFTIKDLDGNTVSLSQY
                     RGKTVLLNIWATWCGPCKKEIPDLIALHNEMKDKDVVVLGVLLESESAEASKPTVKEF
                     NINYPVWYGDDAFAQQFQVQAFPTT"
BASE COUNT         1006 a          817 c          918 g         1032 t
ORIGIN      
        1 taatacagag cgtgtattgt gaaagtatgt acgtacatcg gaacgaatgg cggtgtctat
       61 cagagtatat aagacgccaa aatcatgcgt tcggatcgct gtcaccacgg cattatggca
      121 gttttccggg gtatcggttg aatatgattt tccggaacat gccgttaata aaatggagac
      181 caaggataaa agaaatacgg ttacacgtgc gtaaaaattc atggcgcatg atacaatgat
      241 ttgccgtgac gtgcaagggg cggctaaaaa atcttgttta tcttcaccgg agttaatatg
      301 ttgcaacccg atgaaaaaca ttgcaaaaaa tcaaaattta aagcccgaga cgatcaatat
      361 tttcggcaag acattgtccg aactggaatc gttttttgaa gctttgggcg aaaaaaaatt
      421 ccgtgccgta caggtttttc aatggatgta taaacgcggt gtcacaaatt ttgatgatat
      481 gtcggatttg tccaaatcct tacgggataa gctgcatgca tccgccgtta tcataaccga
      541 taccgtgatc aaaatccaaa cttccggcga aaaagagaac gacgtaacgc gaaaaatttt
      601 atttcagctt gcggacggtg aaaaagttga ggccgtgtat atacccgatg aggggcgtaa
      661 aacggtttgt atttcctccc aggtcggctg tgcggtagca tgccaatttt gcgccacagg
      721 ctggatgggg tttcgtcgca accttagtgt cggcgagatc atcgggcagc ttatgtatat
      781 ccgtcgtgaa atcgatcctg agatatccaa tattgttttt atgggcatgg gcgaaccgtt
      841 tcttaattat gaaaatgtca tgaatgcggc gggcattata atatcggaac atggtcccgg
      901 tttggcgcct aaacgaatca ccatatccac ctcaggtatt atccccaaaa tttatgagtt
      961 tgccgacgcc gatttgcctt acaatctcgc gatcagtgtc aatgccacga cggatgaggt
     1021 acggcaaaaa gtgatgccca ttaccaaaaa atatccgctc aaggatttgc tggaatccat
     1081 ccaatatctc aatgcccggc gaaaaaaccc ggcgacgctg gaatatgttt tacttgcggg
     1141 cgttaatgat tcggatgacg atgccaaacg cttacgtacg atggctaaac aattaggaaa
     1201 atgtaaagtg aatgttatac cgtacaatcc catcgaagga cccgatctga aacgcccttc
     1261 gccggagcat atcgcgcgat ttatgaaatt agtttcaggt atttcctcgc ctttgacatt
     1321 acgtcgctcc cggggcaggg atattgcggc ggcttgcggg caacttgcaa ttaaggaaaa
     1381 acatgatgta taaaacaaag cgaacgattg tcgctattct tacatactta attttctccg
     1441 tcataccggc acgtgcgcaa accaacgtag ttaaactttc aacccgtgaa gcggtcgaaa
     1501 acggccaacc ggttttggaa atcgtagctg atattgattc gaaataccac gtttacggaa
     1561 tggaaaaact cgacggtccc atcgcaacgg aatttaaatt tacgttgccg gaaggtatag
     1621 aactcgtcgg agaagtgatg agcccggagg gaaaacgtca tcatgatgaa ggatttggca
     1681 ttgacgtgac gtggcactcc ggcaaaaccg tatttaagca gaagctcaaa ctcagcgcaa
     1741 cggctaaaac cggtgacata ataaccgtcg cgatgacgta tcaggcgtgt accgaatcgt
     1801 attgccttcc gcctaaaaca atacgggcta cgcataccat ttcgcaacaa tggatcggat
     1861 tatccgcggg aacgataaaa cccataaata aaataaatcc taatcaagcc gttggaagca
     1921 tcagcctaca gagccattcg atcgctgagc ccgaaaacaa aagctctgcg tcccatgata
     1981 gcgttgctca aacagtgccg gataaagttt cgacgataca ggagcgtacg gcctcgtacg
     2041 aaagtttgcg taaagaatcc ttgggctcat tcatcggatt agcatttctt acagggcttt
     2101 tggcgctact cacaccctgc gtctttccga tgattccgat tacggtttca tattttacca
     2161 aacattcggc gtcaagccgg gcgggggcag tgcgtcagtc ggtgatctat gtcgtcggaa
     2221 ttatcgcatc gtttacgtta ttcggatggc ttatgtcgct tgtcgtcggc gctaccgggg
     2281 cccaaaattt tgcagccaac gcctgggtga atctcgcgat cggaatttta ttcatcgttt
     2341 ttgcaatgag tttatttggg gtttttgaaa ttcggctacc gtcgtttatt gtcaattttt
     2401 cgcaacagca agccaatacc ggacaaacga tcggtacgct gatggccggt gttacattta
     2461 ctttggtttc atttacctgt accgtgcagt ttctcgggct tttgatggtg gcggcagccg
     2521 acggagagtg gtttctgccg gtgattggga tgttgtgttt tgcgacggcg ttttcaacac
     2581 cgtttttctt tctttcttta tttccgcaat acctcgctaa tctccctaaa agcggcggat
     2641 ggcttcatgc gacaaaaatc gtcatgggat tggtcgaaat agccgcggcg ttcaaatttt
     2701 tcagtaatgt tgacttagtc tgggatctgc aatggctcac acgaccggcc atgctttcga
     2761 tatggatcac attgtttggc gtcagtggta tttatctgtt ggggaaaatt cgctggtctg
     2821 tcgaagatga agtcgaagaa ataggttttg gtcgtacaat gaccggactt ttatttattt
     2881 cgtttacggt ttacctgctt agcgggcttt ttggaaatcc gttacaagct gatatggatg
     2941 cgtatttgcc tccgtcggat tatggcgtag tgcgcacggc cggaaatgcc aagagtacag
     3001 agtcacacga aacatggatc gaagactacg aagccgccct tcgtgaagcc cgtacttccg
     3061 gcaaaccctt gtttattgat tttaccggca agacctgcac caattgtcgc ctcatggaaa
     3121 aaaccatgtt tacgcgcgct gatgttcaat tctattttga tcgtatggtg ttgacgcggt
     3181 tgtggactga ttttggagaa aacatggaac gttatcagga cttgcagcag cgtttagtcg
     3241 gcagtgtagc tttgccgttt tacgttatca tcacgcctga cgaaaaagta ttggctcgtt
     3301 tcggcgggct tgaacgcgac cctgaaattt ttaaatcatt tttagctgac ggactacaaa
     3361 aaagtcaaaa ataacttcat aaggagtttt tatgcgttgg tctcttgcga tatttgtttt
     3421 ggcaggactc atgtcatcgg gttgcaataa acccggatcg ggtggcgccg tggattttac
     3481 gatcaaagat cttgacggga ataccgtttc gctttcacaa taccgcggca aaacggtttt
     3541 attaaatatt tgggcgacgt ggtgcggacc atgtaaaaaa gaaattccgg atctcatcgc
     3601 tttgcataac gaaatgaagg acaaagatgt agtcgtactc ggtgttcttc tcgagtccga
     3661 atccgccgaa gcttcgaaac cgacagtgaa agaatttaat atcaattatc cggtatggta
     3721 tggtgacgat gcgttcgccc agcagtttca agtgcaggcc tttcccacga cgg
//
LOCUS       DAOQNQ010000306         3750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_11539_length_3750_cov_6.069012, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000306 DAOQNQ010000000
VERSION     DAOQNQ010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11539_length_3750_cov_6.069012"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1723
                     /locus_tag="PK754_13880"
     CDS             <1..1723
                     /locus_tag="PK754_13880"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNB10639.1"
                     /translation="TLFVMANSIFAFALKPGRFLWKYPQGIRFDYTMVAESGRLYIVG
                     FDQIQIFVGGSGERMAYWRMPALTDLNYIRIARGIFWSGYDSIAYNLNALLSNRPSHS
                     EIDAWFQIGRSYEDAGQTDSALCYYDDVLKARGEYIEALDHKSSILNQTGKTSEAVKV
                     ALDAVRYGVPCRSRDLLYACTGLSGYTSVPGWMSHVETADTMAYIGVGTPGFVGKAFS
                     KIYGIHLRRMQSDWAFDFQCGSYMPSFTMNKRTLFAYKPVENKYALIAIARLDGQLLW
                     QNDLRASYIPSEYYDEFIRPAASDNVVVSVPGSVSGWNASDGKRLWQITQSAETFDEN
                     PDGWVLPAEMNEGDFNMSYLGGTPLAINHRFYVSSPKTLWCISEEGKKLWQTSTAHHK
                     LSLRYENSMISVQDFIASDTAKRFYDPMYGRRLASAPDREFHRVHKDETHSAFDGKHV
                     YTLEEYANGITVKKQGSGVPAHSEVFQLTNYSTDMGKWITLADDEIWVLLGYLNQDGT
                     DYGGVVVLDAPTLRCKWKLDIGKNVVHMFATKDAYFIFCSRGDIIRMSKTKMNELLAR
                     GQLWWKK"
     gene            1770..>3750
                     /locus_tag="PK754_13885"
     CDS             1770..>3750
                     /locus_tag="PK754_13885"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNB10640.1"
                     /translation="MSRRVFSVIGFLFCVAILPVYGQIKSVAVMRFENRSSFPENEWI
                     GVGFSETIATKLSLVETITMVERVKLNEVLAANKKTDLLSDSQKVEAEEARTIIGVEY
                     LLIGSVQTLGSYKDLNSKIKISARIISTKSAAILGDAAFTLDGTVKDLFDLQSKIAAK
                     FCAAIGVSATASAYNFEDAMNLTAQQSFGEGMNAFYKHDYKKAIDFFRQALNSNEGSF
                     YASAHTMEGTARQKQIESAAAGDQDKLKKEYLEQFRTDASQAAPAFYDLGTAYKATEN
                     WSEAIKAYSEFLKYAGTNAKVVLWKKVIKGGCFTLGDNKVYAGTDGPPAMTTYDLESG
                     NLLSELKLKGEFVCRDLMYANGHVIWDGWSRIMSFDPTKPDPLWTYKENFDERSPLLI
                     GNGVLAWLSEKTIYGVDLQTGKKLWSTSSETKKPSGILLNQKTAYVNNDGNFERDAYV
                     SKYDFTTKKASRAGTEKVYAIGPQYSLYLSSNYVKGIENETGNQSWSVWDTDISPKKQ
                     QPHFQMAVADSQRFYLVSRDADITSQYSESGLSKKFIVGLDAATGNALWYHAVDGFLS
                     SQMVSSHGLLFVATRYGNAEKYQDKKRINIYALDAVTGEKVWNHEVPEQVTKLEVKDD
                     YLLAVTERNSDTDKKYVYTFRITNMKKPLLLYSDLD"
BASE COUNT         1065 a          717 c          947 g         1021 t
ORIGIN      
        1 tacattattt gtcatggcca attccatttt tgcttttgca ctcaagcctg gtcgtttttt
       61 atggaaatat ccgcaaggca tacgttttga ttacacgatg gtcgcggaaa gcgggaggtt
      121 gtatatcgtt ggtttcgatc agatacagat ttttgttggt ggttcgggtg agcgcatggc
      181 gtattggcgt atgccggcgt tgacggactt aaattatata cgcatcgcac gagggatttt
      241 ttggtccgga tatgacagca ttgcgtacaa tctaaatgcc ttattgtcca accgtccatc
      301 gcattccgaa atagatgcat ggtttcagat aggccgaagt tatgaagacg cgggtcagac
      361 ggattcggct ttgtgctatt atgacgatgt tttaaaagca cgtggggaat acatcgaggc
      421 gcttgatcat aaatcctcta tactcaacca aaccggcaaa acatccgaag cggtgaaagt
      481 agctttggat gctgttcgtt acggtgtgcc atgccgaagc cgtgatttat tatatgcatg
      541 taccggcctt tccggatata catctgtacc gggatggatg agtcatgtgg aaacggcgga
      601 cactatggcg tatatcggtg tcggtacgcc gggatttgtc ggaaaggctt tttcaaaaat
      661 atacggtatc cacctgcgcc gaatgcaatc ggattgggca tttgatttcc aatgcggcag
      721 ttatatgcct tcattcacta tgaataagcg cacacttttt gcgtacaaac ccgtagagaa
      781 taagtacgca ctaatcgcga tcgctcgttt agacgggcaa ctgttatggc aaaacgatct
      841 ccgggcgtca tatattccgt cggaatatta tgatgaattt attcgtccgg cggcaagtga
      901 taacgtggtc gtgtctgtgc cgggttccgt ttcgggttgg aatgcatcgg acggaaaaag
      961 gctatggcaa ataacacagt ccgcggaaac attcgatgag aacccggacg gttgggtttt
     1021 gccggcggag atgaatgagg gagattttaa tatgtcgtat ctgggtggaa cgccgcttgc
     1081 gatcaatcac cgcttttatg tgtcatcacc caaaacgcta tggtgcattt cggaagaagg
     1141 aaaaaaacta tggcaaactt cgacagcgca tcacaaacta tcgcttcgtt atgaaaatag
     1201 catgattagc gtacaagatt ttatcgcttc agatacggcc aaaaggtttt atgatcccat
     1261 gtatgggcgt cgtcttgcat ctgcgccgga tcgtgaattt catcgggtgc ataaagatga
     1321 aacacattcc gcttttgacg gaaagcatgt ttacaccctt gaagaatatg ccaatggaat
     1381 aactgttaaa aaacaaggat cgggtgtgcc ggctcactca gaagtttttc agttgacaaa
     1441 ttatagcacg gacatgggaa aatggattac attagcagac gacgaaattt gggtgctact
     1501 cggttatctc aatcaggatg gtacagatta cggcggtgtg gtggttttag atgctcctac
     1561 gttgcgatgt aaatggaaat tggatatcgg gaaaaatgtt gtacacatgt ttgcaacaaa
     1621 ggatgcgtat tttatttttt gttcgcgcgg cgatattatt cgcatgtcta aaacgaaaat
     1681 gaacgaactt ttagcgcgtg gtcaattgtg gtggaaaaaa taaggttcaa tttttaaata
     1741 ttcacactaa aatgaaaggc gagaatatta tgagcaggcg agttttttct gttatagggt
     1801 tcttgttttg tgtggccatc ctgccggttt atggacaaat caaatcggta gcggtcatgc
     1861 gttttgaaaa ccggtcatcg tttcctgaga acgagtggat cggcgttggg ttttcagaaa
     1921 ccattgcaac caagttgtcg ttggtcgaaa ccatcacgat ggttgagcgt gtgaaattga
     1981 atgaagtgct tgcggcgaat aaaaaaacag atttgttgtc cgattcacaa aaagtggaag
     2041 cggaagaagc gcgtactatt atcggcgttg aatatctgct tatcggttca gtgcaaacgc
     2101 tgggttcata taaagatctg aattcaaaga ttaagatcag tgcgcgtatc attagcacga
     2161 agtcggcggc gatcttgggc gatgcagctt ttacattgga cggcacggta aaggatttat
     2221 ttgatttaca atctaaaatc gctgcaaaat tctgtgctgc tataggtgtc agcgctacgg
     2281 cctctgcgta caacttcgaa gatgctatga atctcacggc tcaacagtcg ttcggagaag
     2341 gtatgaatgc gttttataag cacgattata aaaaagcgat cgactttttt cgccaggcgt
     2401 tgaattctaa cgaaggaagt ttttatgcct cagcgcatac catggaaggt acggcacgac
     2461 aaaagcagat cgaatccgct gctgcgggtg atcaagacaa actgaaaaaa gaatatttgg
     2521 aacaatttcg caccgatgca tcgcaagcgg caccggcatt ttacgacctt ggaacggctt
     2581 ataaggcgac ggaaaactgg agcgaagcca tcaaagcata ttctgaattc ttaaaatatg
     2641 ccggcactaa cgccaaagtc gtgttgtgga aaaaagttat aaaaggcgga tgttttactt
     2701 tgggtgacaa taaagtgtac gccggcacgg atggccctcc ggctatgacc acctatgatt
     2761 tagagtcggg taatctttta tcggagttaa aactgaaggg tgaatttgtt tgcagagatt
     2821 tgatgtatgc taacggccat gtcatctggg acgggtggag ccgtattatg agttttgatc
     2881 cgactaaacc ggatccgttg tggacctaca aagaaaattt tgatgaacgc tcgcctttat
     2941 tgatcggaaa tggtgtttta gcatggcttt ctgaaaagac aatttatggc gttgatttac
     3001 aaaccggtaa aaaattatgg tccacgagct cggagactaa aaagccaagc ggcattttgc
     3061 tcaatcaaaa aaccgcgtat gtcaataatg atggtaattt cgagcgtgat gcctatgtaa
     3121 gtaaatatga ttttacgacc aaaaaagcat cgcgcgccgg taccgaaaaa gtttatgcga
     3181 taggaccgca atatagcctg tatctgtcca gtaactatgt caaagggatt gaaaacgaaa
     3241 ccggtaacca atcatggagt gtgtgggaca ccgacatttc tcccaaaaag caacaaccgc
     3301 atttccaaat ggcggtggcc gacagccaac gtttttatct tgttagccgg gatgccgaca
     3361 taacatctca gtacagcgaa tcgggattga gtaaaaagtt tatcgtaggc ctagatgccg
     3421 caacgggcaa tgcgttatgg taccatgccg ttgacggatt tttatcctcg cagatggtta
     3481 gttcgcacgg gttgcttttt gtggccacgc gatacggtaa tgcggagaaa tatcaggata
     3541 agaagcgaat aaacatctac gcgctcgatg ccgtgactgg agaaaaagtg tggaatcatg
     3601 aagtgccaga acaggttaca aaactggagg tcaaagacga ttatttgctg gcggttaccg
     3661 aaagaaattc agacacggat aaaaagtatg tttatacgtt tcgcataacc aatatgaaga
     3721 aaccgctttt attgtatagc gatctggatg
//
LOCUS       DAOQNQ010000307         3750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_11541_length_3750_cov_5.667118, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000307 DAOQNQ010000000
VERSION     DAOQNQ010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11541_length_3750_cov_5.667118"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PK754_13890"
     CDS             <1..435
                     /locus_tag="PK754_13890"
                     /inference="COORDINATES: protein motif:HMM:NF036547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB10641.1"
                     /translation="IDAFENDADDTMLPIPTHMTPEQAERELLYRTLLSLKRDVDDIK
                     HFLVAKFGNVPRPLGGSDFPHAGYAPHFDKETRDGKIVETEVYNVDEGQERDSLSLSD
                     LERDMIIKALKKFDGKRNAAARSLGLSERTLYRKIKEYNLDL"
     gene            432..959
                     /locus_tag="PK754_13895"
     CDS             432..959
                     /locus_tag="PK754_13895"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HNB10642.1"
                     /translation="MNVEPVHVTMKSYFKYLFFGILLIGLIGCVYSFTGANLGELKTV
                     AVPMFDNLTAEPGIREKVTTGLTQGILDDNNLRIADFKSADAVLSGKITQVDDIVFAF
                     EGSGNQFTTSDYKITITAIIKFENRREKKTMWEDTFSGWGRYALTGTRKRSDGMDDAI
                     KMLTQNVINKVTSDW"
     gene            1084..2535
                     /locus_tag="PK754_13900"
     CDS             1084..2535
                     /locus_tag="PK754_13900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB10643.1"
                     /translation="MTVLFVFSMHNFIMIYYFLKHRDDHPQPSEDWVHLPEEKLPVIT
                     IQLPIFNESTVAERLILATSQLDWPKNKLEIQVLDDSTDDTVQLTRACVERLAAQGYM
                     IRLFHRTDRKGYKAGALREALEHAHGEFVAIFDADFIPEKDFLRKAMPHFSQSRIGMV
                     QSRWGYLNEDFSMLTKTQAIGLNGHFVIQQLARNRAGFFINFNGTGGIWRKSCITDAG
                     NWHDDTLTEDLDLSYRAQLKGWKFVYLNDIVVPGELPIGIHALKTQQFRWTKGSVETA
                     KKLVPKILKAELPMWTKIQSLFHLTANFSYIFVVISCLLNSSILFLHNENGRFNVILD
                     IMAVFVLSMLGLFIFFFYSEKMINPNWKKKMIILPVLMMGSIGLSISNAKAVIEGFFD
                     IKSSFVRTPKWGTMKRKEKTRFHGVKLDALFLIELLFLAFILVSLVVGIRNYYTEKAN
                     FIGMIFFNVWILVGFSMVVFLSLKHFYDNRASA"
     gene            2556..>3750
                     /locus_tag="PK754_13905"
     CDS             2556..>3750
                     /locus_tag="PK754_13905"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB10644.1"
                     /translation="MAKAEKQAVPAVGNKAILAEVTLIDAIKQIESKLAYQPESVRFA
                     QLADAYLDMGQYDQAITILTDGLQKFPYYDTARLILAKAYYRRGEKKQAQEYIREFLQ
                     SHPAHVAAHKLLGDLALEEDDVVSAVKKYRVALRFDPINKVLIQSLVDLKDQYQKLKA
                     SRSEEEDEDGVRPVVRKPVSSAERPAPTAETPVAKAQPSFEPPAEKEELKMPTEMVED
                     LIVKEMTKKKAVPVAEVVKPEPLAEPVLEEKRDELISDSASEVPTTVKQTAAETSDVD
                     EALKASAAWIDSEDVLYFYDDEEVSFAEFKQRQELKKAGRAAIMDRAALDKKIAAAGG
                     TVKKQTVPESPKEKTEEIRAKIETPDVKEVPTAPSVKKEIPNPEEKPASQPKVVKRPP
                     ERKTDD"
BASE COUNT         1044 a          835 c          932 g          939 t
ORIGIN      
        1 attgatgcgt ttgaaaatga cgccgacgat accatgttgc cgattcccac acatatgaca
       61 ccggagcaag cggagcggga actgttgtac agaacgcttc tgtcgctgaa acgggatgtg
      121 gatgatatca aacatttttt ggttgctaag tttggcaacg taccgcggcc tttgggcggc
      181 agcgattttc cgcacgccgg atatgcaccg cactttgaca aagaaactcg cgacggtaag
      241 atcgttgaaa cggaagtgta taacgtggac gaagggcaag agcgcgacag tctttcgctg
      301 agcgatctgg aacgggatat gatcatcaag gcgctcaaaa aatttgacgg caaacgaaat
      361 gccgccgcgc gttccttggg actgtctgag cgcacgttgt atcgcaagat caaggagtac
      421 aatctcgatc tgtgaacgtc gagccggtac atgtaacgat gaaaagttat ttcaaatatt
      481 tgttctttgg catactgctg atcggtctta tcggatgcgt ctattcgttt accggggcca
      541 acctcggtga actgaaaacc gtcgccgttc cgatgttcga taatctgacg gccgaacccg
      601 gtattcgtga aaaagtcacg accggtctga cgcagggtat cttggacgac aataacctgc
      661 gcatagccga tttcaaatct gccgatgccg tgctgagcgg aaagatcacc caagttgatg
      721 atatcgtgtt tgccttcgaa ggcagcggca atcagtttac cacatcggat tacaagatca
      781 ctattacggc gatcattaaa tttgaaaatc gccgcgagaa aaaaacaatg tgggaagaca
      841 cattttccgg atgggggcgt tacgcactca ccggcacccg caaacgcagt gacggaatgg
      901 atgatgcaat caagatgctc acgcagaacg tgatcaataa ggtcacgtcg gattggtaac
      961 tatgagtaat aaaaacccta atttttttac cctttaaaaa accctaatac cctaaaccct
     1021 aaactcgaga tgttctttac catggatgca ggttctttct tattctacgc gtacctcgcg
     1081 tgtatgacgg tgctgtttgt tttcagcatg cacaatttca tcatgatcta ttattttttg
     1141 aagcatcgtg atgatcatcc gcaaccgtca gaagactggg tacacttacc ggaagaaaaa
     1201 cttccggtga tcacgattca gttaccgatt ttcaatgaaa gtacggtagc cgaacggctg
     1261 attttggcga ccagccagtt ggattggccg aaaaacaaac tggaaattca ggtgctggat
     1321 gattcgacgg atgatactgt ccaattgacg cgggcatgcg tcgaacgctt ggccgcacaa
     1381 ggttacatga ttcgtctgtt tcatcggacg gatcgaaaag gttacaaagc cggcgctttg
     1441 cgcgaagcgc tggagcatgc gcacggcgaa tttgtcgcga tctttgatgc ggattttatt
     1501 ccggaaaaag atttcctgcg taaagcgatg ccgcattttt cccaatcccg gatcggtatg
     1561 gtgcaaagtc gctggggcta tttaaacgaa gatttttcga tgctcactaa aacacaggcg
     1621 atcgggctca acggacattt tgtgattcaa cagttggcgc gtaaccgtgc aggctttttt
     1681 attaatttca atggtaccgg cggtatctgg cgcaaatcct gtattaccga tgccggtaat
     1741 tggcacgacg atacactgac cgaagatttg gatttgagtt acagagcgca gctcaaagga
     1801 tggaagttcg tttatttgaa tgatattgtg gtgcccggcg aattgccgat cggtatccat
     1861 gcattgaaaa cgcaacaatt ccgctggacc aaaggttccg tggaaacggc gaaaaaatta
     1921 gtacctaaaa ttctgaaagc cgaactgccg atgtggacca aaatccaatc gctttttcac
     1981 ctcacggcca atttttcgta catttttgtc gtgatttcgt gtttgctcaa ttcttccatt
     2041 ttgtttttac acaacgaaaa cggacgcttt aatgttattc tcgacattat ggcggtattc
     2101 gtactcagca tgttgggtct gtttatattt ttcttctact cggaaaaaat gatcaacccc
     2161 aactggaaga aaaagatgat tattttgccg gtgcttatga tgggaagtat cgggctttcg
     2221 atcagtaatg caaaagccgt aatcgaaggt ttttttgata tcaagtcgtc cttcgtacgt
     2281 acgcccaaat ggggtacgat gaagcgcaaa gaaaaaacac gttttcatgg cgtgaaattg
     2341 gatgcacttt ttttgataga attgttgttt cttgctttta ttttggtatc tttagtagtc
     2401 ggtattcgca actactatac cgaaaaagcc aacttcatcg gcatgatctt ttttaacgtt
     2461 tggattcttg tcgggttttc gatggtggta ttcctgtcct tgaagcattt ttacgacaac
     2521 cgagcctcgg cataacgcga atatcaattt ttcctatggc caaagcagaa aaacaggcgg
     2581 tccccgccgt cggcaataaa gcgattctcg ctgaagtgac gctcattgat gcgatcaagc
     2641 agattgagag caaactggcg tatcaaccgg agagtgtgcg cttcgcacaa cttgcggatg
     2701 cgtaccttga catggggcag tacgatcagg cgataactat tcttaccgac ggactacaaa
     2761 aatttccata ctatgatacg gcccgcctga ttttggccaa ggcgtactac cggcgcggtg
     2821 aaaaaaagca ggcgcaggag tatattcgtg aatttctcca atcgcatccg gcacacgtag
     2881 cggcgcataa attactcggt gatttggcac ttgaagaaga tgatgtcgtt tctgcggtaa
     2941 aaaaataccg cgtggcgttg cgcttcgatc cgattaataa agtactgatc caatcgcttg
     3001 ttgaccttaa agatcaatat caaaaactga aggcttcgcg ctctgaagaa gaagacgagg
     3061 acggcgtgcg tcccgtggtt cggaaacctg tttcatccgc ggaacgccct gcaccgacag
     3121 cggaaacacc tgtagcaaaa gcgcaacctt cctttgaacc tccggctgaa aaagaagaac
     3181 tgaagatgcc gaccgaaatg gtcgaagatc ttatcgtcaa agaaatgacg aagaaaaaag
     3241 cggttccggt cgcggaggta gtaaagcctg aacccttggc agaaccggtg cttgaggaaa
     3301 aacgtgatga attgatttcg gattcggcga gcgaagtacc aacgacagtc aaacaaaccg
     3361 cggccgaaac gtccgatgtg gatgaagcat taaaagcgtc ggcagcgtgg atcgatagcg
     3421 aagacgtttt gtatttttat gatgatgagg aagtgtcgtt tgctgaattc aagcagcgtc
     3481 aggaactgaa aaaggccggt cgtgcggcga tcatggatcg tgcggcgctg gataaaaaaa
     3541 tagccgccgc cggaggtact gtcaaaaagc agaccgttcc ggaaagtcca aaagagaaaa
     3601 cggaagagat tcgcgccaaa atcgaaacac ctgacgtcaa agaagtgccg acggcgccat
     3661 ctgttaaaaa agaaattccc aatcccgaag aaaaacccgc atcgcagccc aaagtcgtca
     3721 aacggcctcc tgagcgtaaa acggacgact
//
LOCUS       DAOQNQ010000308         3713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_11734_length_3713_cov_5.443685, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000308 DAOQNQ010000000
VERSION     DAOQNQ010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11734_length_3713_cov_5.443685"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="PK754_13910"
     CDS             complement(<1..958)
                     /locus_tag="PK754_13910"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNB10645.1"
                     /translation="MSTQKPLSGTELMDKIVSLCKRRGFIFQSSEIYGGLNGCWDYGP
                     LGVELLKNYKDAWWKAMTFRDDIEGLDAAILMHPRVWEASGHVENFTDPMVDCKECKA
                     RFREDQIEDSMCGNKAYQGRKAHKCMAEGKFTEARQFNLMFKTYIGPLEDSSAVVYLR
                     PETAQGIYVNFHNVKDSSRQKVPFGIAQIGKAFRNEINTKNFLFRTREFEQMEMQFFV
                     KPGTDMEWFNTWKEIRKQWYLSLGFTEDKLKFHAHEKLAHYAAAAFDIEFLFPFGWGE
                     IEGIHNRTDFDLKRHQEYCGKNLDYFDDTTKEKYLPYVIETSA"
     gene            1158..2396
                     /locus_tag="PK754_13915"
     CDS             1158..2396
                     /locus_tag="PK754_13915"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB10646.1"
                     /translation="MRMTFFENFRSAVSFTMHNVKARFFHTFLSVLGIVIGVASLVMV
                     LALIEGMEASVRKQILQTTSMNMVIVSSEPFKKSDGVRMRKTDLAILSPVVFDSLKSS
                     LRISHTAQLVSTYGAEVKVDSSKWVGAQINFSSAMVLRTDSLLAGRVLSDEDIHDKKK
                     FAVINRFLAFQSAKDSSLIPALIGKSIHIGAQTYTIMGITAARETTAEAVLPISFMDS
                     AKLTEYPPIGLFETPQIENVIPLQEHIQNYLHTHPVSTGDTLIVASNKSRVDQMAKGF
                     LVFRIVMGFIVGISVLVGGIGVMNVLLISVTQRTMEIGVRKAAGAKRRDILVQFLSES
                     VVVSFLGSVLGLILGYLGAMLLAVIISHMATTPFQASVTWGTLLTVSGIAVMVGIIFG
                     TYPAALASKLNPAEAIRHEG"
     gene            complement(2384..>3713)
                     /locus_tag="PK754_13920"
     CDS             complement(2384..>3713)
                     /locus_tag="PK754_13920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB10647.1"
                     /translation="DPGLPPLEFWRKRITIAVVAANCIMGTAALIPTIPFFMQSETPW
                     IGLIFVVMMIGMYFAWFSQKPSQLKKSILVTVSFLIAGTLCFKPFGAAGAICTLFVAH
                     ILSSLLFRPRFFLISLFYILIVFIAAGYYMEAGNVIGEDRFESRHTWNTWFIVIANVT
                     AAFLTTGWAIHVVINSLEHSLIMENTSRIALQEEINQHNRTEFERKKLEEEVFHMQRA
                     QAMHQIAGGIAHDFSNVLTSTLGYLELLQERVQHDPKAVEMISSMDAGARRGRQLIRQ
                     LLMYSRQTEVHHETFQVVPLIDECYGMVKPALKHIQYQFQSEAQDDTIYGDPTQMHQV
                     FFNLMTNAIQAMNGKGTLSVKVISHLNRNLRIVVSDTGPGIPAENIDKIFEPYFSTKP
                     QELGTGLGLAVVQSIIRNHHGHIRVQSSPGQGATFEIDLPLQKLNTDQPS"
BASE COUNT          983 a          804 c          852 g         1074 t
ORIGIN      
        1 ccgccgatgt ttcgatcaca tacggcaagt atttttcttt ggtcgtatcg tcaaaataat
       61 ccagattttt accgcaatat tcctgatggc gcttgagatc aaaatccgta cggttgtgta
      121 taccttcaat ttcgccccat ccgaagggga aaagaaattc gatatcaaaa gccgctgccg
      181 cataatgcgc cagtttttca tgcgcgtgaa attttagttt gtcttccgta aagccgagtg
      241 aaaggtacca ttgtttacgg atttctttcc acgtattaaa ccattccata tccgtaccgg
      301 gcttcacaaa aaactgcatt tccatttgtt cgaattctcg cgtacggaat aaaaagtttt
      361 tggtgttgat ctcattgcgg aaggctttgc cgatctgtgc gataccaaac ggtacttttt
      421 ggcgggaaga atctttgaca ttgtgaaaat tgacatagat gccttgcgcc gtttccgggc
      481 gaaggtaaac taccgcgctg gagtcttcga gcggtccgat gtaggtcttg aacatcagat
      541 taaattgtcg cgcttcggta aactttcctt cggccatgca tttatgtgct ttccggcctt
      601 gataggcttt attgccgcac atcgaatcct cgatctggtc ttcacgaaaa cgcgctttac
      661 attctttgca atccaccatc ggatcggtaa agttttccac gtgaccggag gcttcccata
      721 cgcgcggatg cataagtata gcggcatcca aaccttcgat atcgtcccga aaggtcatgg
      781 ccttccacca agcatcttta taatttttca gaagttcgac gccgagcgga ccgtaatccc
      841 agcatccgtt gaggccgccg taaatttccg acgattgaaa aataaatccg cgccgcttgc
      901 atagcgatac gattttgtcc atgagttctg ttcccgaaag aggcttctgc gtgctcatac
      961 tgctccgttg accaacgtga atccgcacat aggatgtgtg cggacataat taattttaac
     1021 gacggattat attgacattc acgatgattt gcaatttgaa actacaaccg atgaataggc
     1081 aaaaaaattt gacagggaac tttttgaatt gaaaacgtat accatgcaga taaaacagat
     1141 ttaaaaaccg ggttgttatg cgaatgacat tttttgaaaa cttccgtagc gccgtttcgt
     1201 ttacaatgca taatgtgaag gcgcgttttt ttcatacttt tctttcagtt ttgggaatcg
     1261 taatcggtgt agcgtccttg gtgatggtgc tggccctcat cgaaggtatg gaggcatccg
     1321 ttcgtaaaca gatactgcaa actacgtcca tgaacatggt catcgtgagc agcgagccgt
     1381 ttaaaaaatc agacggtgtt cgtatgcgta aaacggactt ggctatactg tcaccggtcg
     1441 tatttgattc gctgaaaagt tcactgcgta tttcccatac tgcgcaatta gtttccacgt
     1501 atggtgcaga ggtaaaagta gattcatcca aatgggtagg cgctcagatc aattttagtt
     1561 cggccatggt tttaaggaca gattctttgt tggccggccg ggtattgtcg gacgaggata
     1621 ttcacgataa aaagaaattt gctgtcatta atcgctttct tgcttttcaa tctgcgaaag
     1681 attcgtcttt gattcctgcg ttaatcggca aatcgataca tatcggcgcg caaacttata
     1741 ccataatggg tattacagcc gcacgtgaaa ctaccgccga agcagtattg ccgatttcgt
     1801 ttatggattc agcgaagctt accgagtacc cgccgattgg tttgtttgaa acaccgcaga
     1861 tagaaaacgt gataccgctg caagagcata tacaaaatta tctccatacg caccctgtca
     1921 gtacgggaga tacattgatc gtagccagta ataaatcacg ggttgatcag atggccaaag
     1981 ggtttcttgt atttcggatt gtgatggggt ttatcgtcgg catttcagtg cttgtcggcg
     2041 gtatcggtgt gatgaatgta ctgctaatct cagtcacgca acgtacgatg gaaatcggtg
     2101 ttcgcaaagc tgctggtgca aaacgccgcg atatattggt tcagttttta tcggaatcgg
     2161 tagtggtatc gtttttgggg agcgttttgg ggttgatttt aggatatctc ggcgccatgc
     2221 ttttggctgt gatcatcagc cacatggcta caacaccgtt tcaagcctcc gttacgtggg
     2281 gcacattgct gaccgtctca ggtatagctg tgatggtggg aattattttt ggtacatatc
     2341 ccgccgcttt agcatccaag ctaaatccgg cggaggccat ccgtcatgaa ggttgatccg
     2401 tattaagttt ttgcaaaggc aaatcaattt caaatgtggc gccttgcccg ggtgaggatt
     2461 gaacacgaat atggccatgg tgatttcgta taatactttg taccacggcc agccctaatc
     2521 ccgtaccaag ttcttgcggt ttagtactga agtaaggttc aaaaattttg tcaatgtttt
     2581 ctgctggaat ccctgggccg gtatcagaaa ctacaatccg aaggttccgg tttaggtgag
     2641 aaattacctt gacgctcaaa gtacctttcc cattcatggc ctgtatagca ttggtcatga
     2701 gattaaagaa gacttgatgc atttgtgttg ggtcgccata gatagtgtca tcttgtgctt
     2761 cggattgaaa ttgatactgt atatgcttta gagcaggttt gaccatacca taacactcgt
     2821 ctatcaacgg cacaacttga aaagtttcat gatgaacttc ggtttgccgg ctatacataa
     2881 gcaactggcg aataagttgg cgcccacgcc gcgctccggc atccatcgaa cttatcattt
     2941 cgaccgcttt agggtcatgc tgaacccgtt cttgtagaag ctcaagataa ccgagtgtac
     3001 tggttaggac attactaaaa tcgtgtgcta taccaccggc tatctgatgc atagcttggg
     3061 ccctctgcat atggaacact tcttcttcta atttcttacg ttcgaattcc gttcgattgt
     3121 gctgattgat ttcctcttga agcgctatac gggatgtatt ttccatgatc aaggaatgtt
     3181 ctaaactatt gatcacaacg tgaatggccc aaccggtcgt caagaaagct gcagtaacat
     3241 tggctatcac tataaaccag gtattccatg tatggcgcga ttcaaaccga tcttcaccga
     3301 taacatttcc tgcttccatg taataaccgg ccgcaataaa gacgataaga atgtaaaaaa
     3361 ggcttataag aaaaaagcgc ggacgaaaaa gtaatgacga taagatatgt gcgacgaata
     3421 gagtgcatat tgctcccgcg gcaccaaagg gtttaaaaca caatgtcccg gcaatcaaaa
     3481 agctgactgt aactaaaata gactttttta attgtgatgg tttttgagaa aaccacgcga
     3541 aatacatgcc gatcatcatc acaacgaaaa taaggcctat ccatggcgtt tcgctttgca
     3601 tgaaaaatgg aattgtaggg atcaatgcgg cggttcccat aatgcagttg gcagctacta
     3661 cggcgatagt gatgcgtttt ctccaaaatt caagcggagg gaggcctgga tcg
//
LOCUS       DAOQNQ010000309         3712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_11740_length_3712_cov_4.475800, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000309 DAOQNQ010000000
VERSION     DAOQNQ010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11740_length_3712_cov_4.475800"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PK754_13925"
     CDS             <1..609
                     /locus_tag="PK754_13925"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB10648.1"
                     /translation="VWHQIEEAHRQISREIGMDLLSTLERYEEILDERDIYERVTHAT
                     KVSSASDIVIHTYAPRFKKYFKLLNIEWLKKFFTIEPLDRKILSHPETIIKDGGEIFF
                     AEWNGKIVGTCAVLNNKAGIYELAKMAVTEKAQGKHIGWKLGSHAIMWAQKSKGKTMV
                     LETSRKLDAALALYRKLGFVLVANPEPSKYERSTIKMELRLR"
     gene            complement(612..1508)
                     /locus_tag="PK754_13930"
     CDS             complement(612..1508)
                     /locus_tag="PK754_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNB10649.1"
                     /translation="MTDIKKYLQPDVVARLANLEMIARFVVEGFITGLHKSPYHGFSV
                     EFAEHRQYMPGDEIRSIDWKVYGRTSRYYVKRFEEETNLKCHIVLDTSASMTFGTGEV
                     NKLQYGGYLAASLAYLMIHQKDAAGLAIFDKVLRKYLPPRATLGYIKTIEEALQNISH
                     HETTRIAENLHAVAERIKRRGLVILISDLLDEAEDVVHALRHFRHDGHEVIVFHVLDH
                     AERTLDFEGDVVFDDLETGEQLATQPWHIRKAYAQEVESFCDYYRRRCRESRVDYVLL
                     DTKTPFDVALREYIAKRSKIGG"
     gene            complement(1565..>3712)
                     /locus_tag="PK754_13935"
     CDS             complement(1565..>3712)
                     /locus_tag="PK754_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10650.1"
                     /translation="PDGKKIVFSGMRFGQSDVFEFELASGKLSKLTDDVFSDSRAVYS
                     RDGSKIAFVSDRREVLGKQPDGFKMINFEYRQTDIYVMNADGSGMERITTDEMNDSWP
                     EWGPDNSKLLFTSDRNGVSNIYVADLKEKKNYAITNNLSGIFQPSLSKDGKFLTFSGF
                     NRSGFDVYTLKNPFEMKPVELPITISMKKFRKDHDLPETLADEIGIKKQQNIVSSNEG
                     ESSTVTDPKADSLKTQESKVSYRNFVFAGLEDENNNSSVKDEDEPEEAVELPENVYKN
                     EKGDYRVRRYRVKFSPDMVYGTAGFNTLTGFQGVTQFAFSDMLGDHRLLLGTNLFFDL
                     KNSSFTGSYYYLANRTDYGITAFHSAYAFATDYLDERIDINGNPVFFNGQDGYPDSFV
                     RFRFYGLGLNASRPINKFNRIDFSATQLVLARETSWNARNDSLPPKNSTGLGRSRQSN
                     NTVLTLSLINDNSLNTYFGPFDGQRAQVAITASPGYGQNGLRFVTATVDGRKYFWFKK
                     FYAIATRASFGATFGRNPQRFFVGGLDNWILPRFNNGDILVDTFEDFLATYVSPLRGS
                     NYYELQGTRYFISNFEFRFPLVHFMQLGFPIPMFLQQIRGVTFVDFGAAWGGEDREFV
                     VDQLTGETKKANYRYIDRNGNIVYNKSRFDWGQTLKGEGNPVFQDARIGYGFGIRAFV
                     GFFILRYDLAWNIRNPRFSGGNAKHYFSIGSDF"
BASE COUNT         1117 a          865 c          713 g         1017 t
ORIGIN      
        1 gtatggcatc aaatcgaaga agcacaccgt cagattagcc gcgaaatcgg tatggattta
       61 ctttccacgc ttgagcgcta cgaagaaata cttgatgaac gtgacatcta tgagcgtgtc
      121 acccacgcga caaaagtttc ttctgcctct gatattgtaa ttcatacgta tgcaccgcgt
      181 ttcaaaaaat atttcaaatt gctcaatatc gaatggttaa aaaaattttt tacaattgag
      241 ccattggatc gaaaaatact atcccatccc gaaaccatca taaaagacgg cggtgaaata
      301 ttttttgccg agtggaatgg aaagatcgtt ggcacttgcg ctgtactaaa caataaagcc
      361 ggaatttatg aactggccaa aatggctgta accgaaaaag cgcaaggtaa acacatcgga
      421 tggaaactag gatcgcatgc gatcatgtgg gcacagaaaa gcaaaggcaa aacaatggtt
      481 ttagaaacaa gtcgaaaatt ggatgccgcg ttagccttgt atcgcaaatt aggttttgta
      541 ttggttgcta atcccgagcc atccaaatat gaacgttcca cgatcaaaat ggagttgcga
      601 ttgaggtaag attatcctcc gattttagat cgttttgcga tatactccct caaggctaca
      661 tcaaagggcg ttttagtatc cagcaaaacg taatcgaccc ggctttctct gcaccggcgg
      721 cgataataat cacaaaacga ttctacttct tgagcgtagg ctttgcgaat gtgccaaggc
      781 tgcgttgcca attgttctcc ggtttccaag tcatcaaaaa ccacatcgcc ttcaaaatcc
      841 agagtgcgct ccgcatggtc cagcacatga aatacgatca cttcatggcc gtcatgacga
      901 aaatgccgca gtgcgtgcac gacatcctcc gcttcatcca aaagatcgct tatcagaatt
      961 acaagccctc gccgtttgat gcgttcggct acggcatgaa gattctccgc aatgcgtgta
     1021 gtttcgtgat gtgatatatt ctgcaaagcc tcttcgatcg ttttgatata acccagcgtt
     1081 gcacgggggg gcaaatactt acgcaatact ttatcaaaaa tggcaagccc ggcagcatct
     1141 ttttgatgaa tcatcaaata cgccagcgaa gccgccagat atccgccata ctgaagttta
     1201 ttcacctcgc ccgttccaaa cgtcatggat gcacttgtat caagcacaat atggcatttg
     1261 agattcgtct cctcctcaaa ccgcttcacg taataccgcg aagtgcggcc gtacactttc
     1321 caatctatcg aacgtatttc gtcaccgggc atatattgcc ggtgctctgc aaactcaacg
     1381 ctgaaaccgt gatatgggct tttatgaaga cctgtaataa atccttcgac tacgaaacgc
     1441 gcaatcatct ctagattggc tagccgcgca accacatcgg gttgtaaata ttttttgatg
     1501 tctgtcatgt cttaaaaaaa taatcccgca cgaggcggga tcatgatact cagtgtttat
     1561 taaattagaa atccgatccg atcgaaaaat aatgtttggc attacctccg ctaaatcgcg
     1621 ggtttcgaat attccaggcc agatcgtagc gtaagataaa aaatcctacg aacgcacgaa
     1681 taccgaaacc ataacctatg cgtgcatcct gaaatacagg gtttccttcg ccttttaaag
     1741 tttgccccca gtcaaatcgg cttttgttat atacaatatt accatttcgg tctatgtagc
     1801 gatagtttgc ttttttggtt tcgcccgtta attgatctac tacaaattca cgatcctctc
     1861 cgccccaggc cgcgccaaag tcaacaaaag ttacgccgcg tatttgttga agaaacatcg
     1921 gtattggaaa gccaagttgc atgaagtgga ctaaaggaaa acgaaactca aaattggaaa
     1981 taaagtaacg cgtaccttgc aattcataat aattactccc gcgtaacgga gaaacatacg
     2041 ttgcaagaaa gtcctcgaac gtatcaacaa gaatatcgcc attattgaat cgcggaagaa
     2101 tccaattatc caatccaccc acaaaaaagc gctgaggatt gcgtccgaaa gtagctccaa
     2161 agctagcgcg tgttgcgatg gcataaaact ttttaaacca aaaatacttt cttccgtcta
     2221 cggttgccgt tacaaaccgc aggccgtttt gtccatatcc cggacttgcc gtaatagcca
     2281 cttgcgcacg ttgaccatca aacggaccaa aatatgtatt aagcgaatta tcattgatga
     2341 gagataaggt taaaaccgta ttattagatt ggcgcgaacg ccctaatccg gttgaatttt
     2401 taggcggcag cgaatcatta cgtgcattcc atgaagtttc ccgcgcaaga acaagttgcg
     2461 tagcagaaaa atcaattcgg ttaaatttat ttatcggacg cgatgcatta aggcctaaac
     2521 cataaaaacg aaacctcaca aaactatcag gatatccgtc ctgaccatta aaaaagaccg
     2581 ggttaccatt gatatctatt cgttcatcca gataatccgt cgcaaaagca tacgcggaat
     2641 gaaaggctgt aataccataa tccgtacgat tggctaaata ataatacgat cctgtaaaac
     2701 tgctattttt gagatcaaaa aacagattgg tacccagcaa aagcctatga tcgcccagca
     2761 tgtcgctaaa tgcaaattgc gttacgccct gaaaccctgt taaagtattg aagccggcag
     2821 taccatatac catgtccgga gaaaatttta cacgataacg tcgaacacgg taatcacctt
     2881 tttcgttttt ataaacattt tccggaagct ccacagcttc ttccggttcg tcttcatcct
     2941 taactgagga attattattt tcatcttcca atccggcaaa tacgaagttt cggtaagaca
     3001 ccttcgattc ttgtgttttc aaagaatcag ctttagggtc cgtcaccgtt gaagattcgc
     3061 cttcattcga tgagacgata ttttgctgtt ttttaatacc aatttcatcc gctaaagttt
     3121 ctggtaaatc atgatcttta cgaaattttt tcatcgaaat cgtaatcggt agctctacag
     3181 gtttcatttc aaacgggttc ttcaacgtat aaacatcgaa accgcttcta ttgaatccgg
     3241 agaacgtcaa aaatttacca tctttgctga gagagggttg gaaaatacca cttaagttat
     3301 tggtaattgc ataatttttt ttctctttga gatcagcaac gtaaatattg cttacaccat
     3361 ttcgatccga agtgaaaaga agtttactat tatccgggcc ccactccggc cacgagtcat
     3421 tcatttcatc cgtcgtaatg cgctccatgc ccgaaccatc cgcgttcatc acgtagatat
     3481 ccgtttgacg gtactcaaaa ttgatcattt taaaaccgtc gggttgttta cccagtactt
     3541 cccgtcgatc tgatacaaat gcgattttac ttccatcgcg actataaacc gcacggctat
     3601 cgctaaacac atcgtctgtc aatttagata atttaccgct agccaattca aactcaaaaa
     3661 catcgctttg accaaaacgc attccggaaa aaacaatttt ctttccatcc gg
//
LOCUS       DAOQNQ010000310         3686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_11889_length_3686_cov_3.833379, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000310 DAOQNQ010000000
VERSION     DAOQNQ010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11889_length_3686_cov_3.833379"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PK754_13940"
     CDS             complement(<1..184)
                     /locus_tag="PK754_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10651.1"
                     /translation="MLRFFNFCIMMFSFVFLISLASCSSAKETTSSPTSSVVSVRDSL
                     TMKDTVKPETNEQPVQF"
     gene            386..837
                     /gene="rnpB"
                     /locus_tag="PK754_13945"
     ncRNA           386..837
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK754_13945"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            929..1438
                     /locus_tag="PK754_13950"
     CDS             929..1438
                     /locus_tag="PK754_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNB10652.1"
                     /translation="MALNGHVLVLNQNYEPMTICHVKKAFVLVFLGKAEIIEIAEGRS
                     IRSVSHEYPFPSIVRLMSFVYKKRKGIMLSRKNILKRDNYQCQYCGTRSDAMTVDHIL
                     PKVRGGKDAWENLVTACITCNNKKGDRTPDEARMVLMTTPRRPHPLSFIQGHASGHYD
                     NWKQYLFMS"
     gene            1523..2536
                     /gene="trpS"
                     /locus_tag="PK754_13955"
     CDS             1523..2536
                     /gene="trpS"
                     /locus_tag="PK754_13955"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNB10653.1"
                     /translation="MPRIFSGMRPTGKLHLGHLVGALENWVKFQDAYQTVYGIVDWHA
                     LTTGYKDTASLRNYILDTAIDWISAGIDPQKSIIMQQSRVKEHSELHLLFSMITPTPW
                     LIRNPAVKEQARDMGLIETTQDEQMMRIDFGHLGYPVLQTADILIYQADTVPVGEDQV
                     PHIELCREIARRFNFLYSASGFQFPEPQHKLTVTPRLPGVDGNAKMSKSLGNCIYLSD
                     DDATIESQVRKMVTDPQKLRKNDPGRPEVCSVYSYHKVFNTDETQGIAAGCTTGELGC
                     VACKKNLGQKLSQRLEPMRQKRAELVSDLHAVEKIISEGSDKARAIASDTMVSVRRAM
                     KLD"
     gene            2556..>3686
                     /locus_tag="PK754_13960"
     CDS             2556..>3686
                     /locus_tag="PK754_13960"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HNB10654.1"
                     /translation="MRYVLFWILSISTLCAQDQIQPYKDYDNDWLSPQFHKGRRDALA
                     QKMKPHSIAVLFSSEERLRSNDTYYQYKPESNFYYLTGFREPNAALIYLPEGWVVGGK
                     TYSTVMFVPKRDYRYEVYNGRRMGTDGTQKILGIDTAFTNDEFSTQLNKILLQQLAAS
                     KLENVYCFNLREQYDTKTLRNMTTAFTDFRNNPRSLLGYMNSVDFINVEPLIGELRAV
                     KTDEEIVLLKKSIEITNDAHLQMMKSCEPGQKEFEIKAVGEYVFTRLGAEYEGYGSIC
                     GSAENSIILHYTTDRRQMKDGDVLLVDMASEYHGYSADVTRSYPVNGKFTKEQTIIYN
                     IVLKAQQAGIDRIKPNVPYREVADAITKTLEDGLLAIGLIQDR"
BASE COUNT         1074 a          801 c          903 g          908 t
ORIGIN      
        1 aaaactgaac cggttgttca ttggtctcag gtttgacagt atctttcatc gtcaaactat
       61 cgcgaaccga aacgacagaa ctcgtagggg acgaagttgt ttctttggca gaggaacagg
      121 aggcaagcga tatcaaaaaa acaaagctaa acatcataat acaaaaatta aaaaagcgca
      181 acattacaaa ctccttagaa atacattttt aaaccgcggc aatataaagc ataggataag
      241 tattgtcaac caaagtttaa tggacgccgc tttgtttttg aaagacttat caaggtgaga
      301 tattttaaca atgcttaaca aagcgattgt taatgcatca aaatatcata tattgattcg
      361 tctcaaaaag ttctttgaaa gcatgaaaag cagatgcgat gatcgttccg tatcatagag
      421 ataacggaaa ggaaagtccg aactccttaa ggcgcggaac cggccgagat ttccaagaaa
      481 tgcaaggtcg ggtgatgatc gcgaaagcgg tgatcaacgg atagtggaac agaaaataaa
      541 ccgccacgcc gatggtgtgg taagggtgaa accgtgcggt aagagcgcac gtggtgtatg
      601 cgtaagcggc gccatgctaa acccttccgg gagcaagatc aaataagaag aagtcccgca
      661 aggggcgggc cgcctgcctg attcgcaaga gcttcttcgg gtagatcgca ttccgtcgca
      721 tggtatcatg ccggtcatga tacagtgaac gggatttccg gcttgccgga aaagataaat
      781 gatcatccgt atgcttcggc gtacgaacag aattcggctt acgatctgct tttcaatttt
      841 tatttgattg aatagagttt ttgctttatc ttgcgcggtt gttaaggtca tgtattaaac
      901 cggcttcgaa agtgagtgtg gtgaaggtat ggcgcttaat ggacacgttt tggtgctcaa
      961 tcagaattat gagccgatga cgatatgtca tgtgaaaaag gcgtttgttc tggttttttt
     1021 gggcaaagcg gagatcatcg aaattgccga agggcgaagt attcgttcgg tttcgcacga
     1081 gtacccgttc ccgagcatcg tacgactgat gagtttcgtt tacaaaaagc gtaaaggcat
     1141 aatgttgtcc cgaaaaaata ttttgaaacg ggataattac cagtgccaat actgcgggac
     1201 acgtagcgac gcgatgacgg tagatcatat tttaccgaaa gtgcgcggcg gcaaagatgc
     1261 gtgggaaaac ttagtcaccg catgtatcac gtgcaataat aagaaagggg atcgcacccc
     1321 ggacgaagcg cggatggtgc tgatgacaac accccgcagg ccgcacccgc tcagttttat
     1381 ccaagggcat gcgagcggcc actatgataa ctggaaacaa tatcttttta tgtcatgatg
     1441 cgcttgttat cacacactac acaggcgccg cgacagtgta accggcttcg aaagccgatc
     1501 tctcaaacgg acgaaaggtt gtatgccgag aattttcagc ggaatgcgcc cgacagggaa
     1561 attgcatctg ggacatttgg tcggagcatt ggaaaattgg gtcaaatttc aggacgcgta
     1621 tcaaaccgtt tacggtattg ttgattggca tgcgctgacg accggctaca aagacaccgc
     1681 ttctcttcgg aactacatac tcgatacggc tattgattgg atttccgccg gtatcgatcc
     1741 gcaaaaaagt attatcatgc aacagtcccg cgtcaaagaa cattcggagc tgcatttgct
     1801 tttttcgatg atcacaccaa cgccttggtt gatccgcaat cccgctgtca aagaacaagc
     1861 gcgtgatatg ggacttatcg aaacgacgca agacgaacaa atgatgcgca ttgatttcgg
     1921 ccatctcggg tatcctgttt tgcaaacggc ggatatattg atatatcaag ctgataccgt
     1981 acccgtcggt gaagatcagg taccgcatat cgaactgtgt cgtgaaatcg cacgccgctt
     2041 taatttttta tacagcgcat ccggttttca gtttccggag ccccagcaca agttgacagt
     2101 cacaccgcga cttccggggg ttgacggtaa cgccaaaatg agcaaaagcc tcggaaattg
     2161 tatttatctt tccgatgatg acgcaacgat cgaatcgcag gtgcgaaaaa tggtcacgga
     2221 tcctcaaaag ctgcgtaaaa atgatcccgg ccgaccggag gtttgttcgg tttatagcta
     2281 tcacaaagtg tttaatactg acgaaaccca gggtatcgcc gcgggatgta caaccggcga
     2341 attggggtgc gtcgcatgca aaaaaaatct tggacaaaaa ctatctcaac gtttagaacc
     2401 tatgcgccaa aaacgcgccg aattggtatc cgatctccat gcggtcgaaa aaataatatc
     2461 cgagggctcc gacaaagccc gcgctatcgc gtccgatacg atggtttcgg ttcgccgagc
     2521 tatgaaactt gattaacgca cgaaaggaaa aaactatgcg ttacgttttg ttttggatac
     2581 tcagcataag tacgttgtgt gcgcaggatc agatccaacc gtacaaagat tacgacaacg
     2641 attggctttc gccgcagttt cacaaaggac gtcgcgatgc gttggctcag aaaatgaaac
     2701 cccacagcat cgccgtgctt ttttcgtcgg aagaacgcct tcgcagcaac gatacgtatt
     2761 atcaatataa acccgagagc aatttctatt atctgaccgg tttccgtgaa cccaatgcgg
     2821 cattgattta tttgccggag ggttgggttg tcggcggaaa aacatattcg acggttatgt
     2881 ttgttcccaa acgagattac cgctatgaag tatataacgg acgtcgtatg ggtaccgacg
     2941 gcacacagaa aattctcggc attgatacgg cgtttaccaa tgatgaattc agtacgcagt
     3001 taaacaaaat tttactccaa cagcttgccg cttcgaaatt ggaaaatgtg tattgtttta
     3061 atttacgtga gcaatatgac actaagacgc tgcgtaacat gacgacggcg tttacggatt
     3121 ttcgtaataa tccccgaagc cttttggggt atatgaattc ggtggatttt atcaatgtgg
     3181 aaccgttgat cggcgaactg cgtgccgtaa aaacagacga ggaaatcgtc ttacttaaaa
     3241 aatccattga gataaccaac gacgcccatt tgcaaatgat gaaaagctgc gaaccggggc
     3301 aaaaagagtt tgaaatcaag gcggtcggtg aatatgtctt tacgcgcctg ggcgcggaat
     3361 acgaaggata cggcagtatc tgcggttcgg ccgaaaacag tatcattctt cactatacga
     3421 ccgatcgtcg tcaaatgaaa gacggtgacg tattgctcgt agatatggct tccgagtacc
     3481 acggttattc cgcagacgtg acgcgttcgt atccggtcaa cggcaaattt accaaagaac
     3541 aaaccatcat ttacaatatc gtgctcaaag cccaacaagc cggtattgac cgcattaaac
     3601 cgaatgtacc ttaccgtgaa gtagcggatg ccattaccaa aacattggaa gatggtcttt
     3661 tggcaatcgg gttgattcag gatcgc
//
LOCUS       DAOQNQ010000311         3683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_11908_length_3683_cov_5.187431, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000311 DAOQNQ010000000
VERSION     DAOQNQ010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11908_length_3683_cov_5.187431"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..1163)
                     /locus_tag="PK754_13965"
     CDS             complement(159..1163)
                     /locus_tag="PK754_13965"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNB10655.1"
                     /translation="MKYFIITLFCLLTFGELSYAQVRGGNRNRIDKKNTVADDPQVAF
                     RMAFKAYKEKDIPKAQKLLKNTLDKHPTHVLSLILISKILVEQDDLKSAKKFIKTIPE
                     SEHHRPAIARILAEIAYRNGKPLKALQIINRTQSDAASSDDAEFFDNPNQDIASFKKQ
                     LLASTRQSQRKEMPYAKEKPATETHLTIPGDLPTVATERRLRLAIFSLSVAPSLDDTT
                     LGSSTASYLTTAAVQTQCFRVVERENLEKVMIEQDFQMTDAVDTETAVETGYIMGVDA
                     ILVGSVRPMAQGYEVDGRLIRVETGEIIAATNQSISSVSLVRQSAANLMFDLTRAAYK
                     "
     gene            complement(1160..1729)
                     /locus_tag="PK754_13970"
     CDS             complement(1160..1729)
                     /locus_tag="PK754_13970"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNB10656.1"
                     /translation="MNNRIFTISNILSMLRIVFAVPILYFLHQGIEDPTANIYALITI
                     LIAGFTDLFDGYLARRLDQVTELGKIIDPLADKIVSAIILVYLALIRPDFPLWLLIVA
                     IVRDVVIFAAAMYVKQKYNYLFTSNMLGKWAFTVLGLTFAIFVVRNNFSIEWLFQSFL
                     WLTFIMLILSFYVYAQRLWVFMQNQRKNA"
     gene            complement(1722..2717)
                     /locus_tag="PK754_13975"
     CDS             complement(1722..2717)
                     /locus_tag="PK754_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10657.1"
                     /translation="MPSYHKLLFVVLFLGSCKPTQSEAIGGTDTLILFCDSTLSPIIT
                     ASLHKTWFMPRAETAFDVQHQTMDQFTVYRHFRYLLFAGSLDAHDAVSKWIRSTLSPD
                     DEKAVREGQLWILEKESPWAQGQMIWVLCTPDYETMVRRCATEGIMLHRRLEEKRREQ
                     ETVMMQHDYFDAMPVQSADADTFYLYDGFSQQPAPMSKTFFWYRSTERFQEWILFSTF
                     TGDSVNISTSLGRHTQKLLNALPDSLKYRDDFITSDTLYTTSGPLLRMCTVWTTKNRT
                     NTFGGPLWLYVSLTPTNKPKKIGVAMAFGPQNERKHPAMRRLENLIIYNLTRSYE"
     gene            complement(2701..>3683)
                     /locus_tag="PK754_13980"
     CDS             complement(2701..>3683)
                     /locus_tag="PK754_13980"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNB10658.1"
                     /translation="MPVTWQKYVWTVLKISVSLGLMYWAVRSIDLTVMIAIWQSINVN
                     WILASFILLTLSYLLGAWQWQSILAIGDIRLKYPTVVGYYYVGLFFNNFLISGMGGDV
                     LRVYDIHRHTSDPQRLSPALAVVFFDRFVGLLILILYACISGIFLIGQGESLRMFFAI
                     TGLLLLWIFALILLFHRPLAIFLLTPLAWLVPKRLRMRVDHLYSELNRFRTAPQLLMR
                     VFLIAGGVQMLRIIAIAAIGRALGDASSLMFYILFVPMISLAASLPISIGGTGPREQT
                     TVFLFRKIGVSSEIAFSIGFVTYLLSAVSTLPGGFIFMLRKNKAEHAVVS"
BASE COUNT         1118 a          799 c          802 g          964 t
ORIGIN      
        1 cgcgcaaatc ttgatgcgta cgacgcaaat cttccgccaa ttcccggatt ttttcaacga
       61 tcatattttt tgtgaaaggt taaatgtatt cgcaaacgca gaaccgtttg tggcgtgaca
      121 ttaattatat aattaattta cccgatacaa gcaacaaatt atttatacgc cgcacgggtc
      181 aagtcaaaca taagattggc agccgattgt cgtaccagcg atacggatga aatactttga
      241 tttgtcgccg caattatttc gccggtttcc acacgaatca agcgtccatc tacttcgtaa
      301 ccttgcgcca tcgggcgtac acttcctaca aggattgcat ctacacccat gatataaccg
      361 gtttcaaccg ctgtttcagt atcaacggcg tccgtcattt gaaaatcctg ttcgatcata
      421 actttttcta aattttcacg ttcgaccact ctaaaacatt gcgtttgcac ggcagctgta
      481 gtgaggtacg acgccgtcga agatccgagt gtcgtgtcat ccaaagaagg tgcaacagat
      541 aaagaaaaaa tagccagtcg taaacgacgt tcggtcgcaa ccgtaggcaa gtcgccgggg
      601 atcgtaagat gggtttccgt tgccggtttt tcttttgcgt atggcatttc cttgcgttgg
      661 ctttgtcgtg tagaggctag aagttgcttc ttaaacgatg cgatatcttg attggggtta
      721 tcaaaaaact ccgcatcgtc cgacgatgcg gcatccgatt gagtccgatt gattatttgt
      781 aaagccttaa gcggcttacc gttgcgataa gcaatttcag ccaatatacg ggctatcgca
      841 ggacggtggt gttcggattc agggatggtt ttaatgaatt tttttgctga tttcagatca
      901 tcctgttcca ccaaaatttt agaaatcaga ataagactca atacatgcgt tggatgtttg
      961 tctaatgtgt tttttaataa tttttgagct ttgggaatat ctttttcttt gtaagcctta
     1021 aaagccattc gaaaagctac ttgcggatca tcggcaacgg tgtttttttt atcgatacga
     1081 tttcgatttc cgccgcgtac ctgagcgtag gacagttcgc caaatgtcag caaacaaaat
     1141 aaagtaataa taaaatattt catgcgtttt tccgttggtt ttgcatgaat acccataaac
     1201 gctgcgcgta aacgtaaaaa gataaaatta gcataataaa tgtaagccag aggaacgact
     1261 ggaaaagcca ttcgatacta aaattatttc gcaccacgaa aatggcaaaa gtaagcccca
     1321 gtaccgtaaa cgcccatttt ccgagcatat tggaagtaaa aagatagttg tatttttgtt
     1381 tgacgtacat ggccgccgca aaaattacca catcgcgtac gatcgcaaca atcagcaacc
     1441 acaagggaaa atcgggcctg atcagagcaa gataaaccaa aataatcgcc gaaacgattt
     1501 tgtcggcaag ggggtcaatg attttcccca attccgtgac ttggtccaat cggcgcgcca
     1561 aatagccgtc aaataaatcc gtaaaaccgg cgatcaatat agtgatcaat gcgtagatgt
     1621 tggctgtcgg gtcttcgatg ccctgatgta aaaaataaag tataggtacg gcgaacacga
     1681 ttcgcagcat gctgaggata ttggagatgg taaaaatgcg gttattcata cgaacgagta
     1741 agattataaa ttattaaatt ttccaatcgg cgcatagccg gatgttttct ttcattttga
     1801 ggtccgaaag ccattgcgac gcctattttt tttggcttat ttgtcggagt caatgacaca
     1861 tataaccaaa gcggtccgcc aaatgtatta gtgcgatttt ttgtagtcca aacggtacac
     1921 attcgtaaca aaggaccgga tgttgtgtac aaggtatcgc tcgtaataaa gtcatcgcgg
     1981 tatttcaaag aatccggcaa tgcattgagt aacttctgag tatgcctgcc gagcgatgtt
     2041 gatatgttaa ctgaatcgcc tgtaaatgta ctaaacaaaa tccattcttg aaagcgttca
     2101 gttgatctgt accaaaaaaa agttttcgac atcggtgcag gctgttgtga gaagccgtca
     2161 tatagataaa aagtatcggc gtccgcgctt tggacgggca tagcgtcaaa ataatcatgt
     2221 tgcatcatga ccgtttcctg ttcacgtcgt ttttcttcca aacgccgatg cagcattatt
     2281 ccttcagtcg cacagcgacg gaccattgtt tcgtaatccg gagtgcataa aacccatatc
     2341 atctgtcctt gcgcccaggg tgattctttt tccagaatcc atagttgacc ttcgcgaacg
     2401 gctttttcat catcgggcga aagggtggag cgtatccatt ttgaaacagc atcatgagcg
     2461 tcgagcgatc cggcaaagag caggtatcgg aaatgacggt aaacggtgaa ttgatccata
     2521 gtttgatgct gtacatcaaa agccgtctca gctctcggca taaaccatgt tttatgaagt
     2581 gaagccgtaa tgatcggtga taacgtactg tcacaaaaaa gaataagcgt atccgttcct
     2641 ccgatggctt ctgattgtgt cggtttacaa cttcctaaaa ataatacaac gaaaagcagt
     2701 ttatgatacg acggcatgtt ccgctttatt ttttctcagc ataaaaataa aaccaccggg
     2761 caaagttgaa acggcagata aaagatacgt cacgaaaccg atcgaaaaag cgatctctga
     2821 agaaacaccg attttgcgaa aaagaaatac cgtggtttgt tcgcgggggc ccgtgccgcc
     2881 gatggaaatg ggcaaactgg cggccagtga aatcatgggt acaaacagaa tgtaaaacat
     2941 caacgaagat gcatcgccca gcgcccgacc gatggccgct atagcgatga tgcgaagcat
     3001 ttgcactccg cctgcaatca gaaaaacgcg catcaaaagc tgcggtgccg tacgaaaacg
     3061 attcaattca ctgtagagat gatcaacccg cattcttaac cgtttaggta cgagccatgc
     3121 cagcggtgtc aggagaaata tggccaaagg gcggtgaaaa agaagaatca aagcgaatat
     3181 ccacaggagt aaaagcccgg tgatggcaaa aaacattcgc aaggattcgc cttgaccgat
     3241 taaaaaaata ccggaaatgc acgcatataa aataaggatc aataagccca cgaaacgatc
     3301 aaaaaaaacg acggccaaag ccggtgaaag gcgttgcgga tcggaggtat ggcggtgaat
     3361 atcatacact cgcaacacat cgccccccat accgcttatg agaaaattat taaaaaaaag
     3421 tcccacataa taatacccta cgaccgtagg gtatttcaat cggatatcgc cgatcgctaa
     3481 tatgctctgc cactgccatg caccaagcag ataactcaat gtaagaagta taaatgacgc
     3541 taatatccaa ttaacattga tggactgcca aatcgctatc atcaccgtta aatcaatact
     3601 ccgtacggcc caatacatca agcccaagct tacggagatt tttaaaaccg tccatacgta
     3661 tttttgccat gtaacaggca tgg
//
LOCUS       DAOQNQ010000312         3675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_11950_length_3675_cov_7.011602, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000312 DAOQNQ010000000
VERSION     DAOQNQ010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11950_length_3675_cov_7.011602"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PK754_13985"
     CDS             complement(<1..663)
                     /locus_tag="PK754_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNB10659.1"
                     /translation="MSAHDAQSGKRHVTEEEAREVAESARESKWENPSFLREIFLGNL
                     RLDLVHPYPEPKGINRPEFQQFYTEFKKFIEEEVDSDAIDREGKIPMAVRDRLKAMGA
                     FGMKIKKEYGGLEFSQAEYNKIMELICTKDGNVIALLSAHQSIGVPQPLMLFGTDEQK
                     QKYLPRLAKGEISAFALTETEAGSDPAKLHTTAVESEDGQSYILNGEKLWCTNGTIAD
                     IIV"
     gene            854..2824
                     /locus_tag="PK754_13990"
     CDS             854..2824
                     /locus_tag="PK754_13990"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNB10660.1"
                     /translation="MFNFSRTLLRDVILYAVLVAIFIAIFLIGEYISDSYFPRTSDYV
                     IFFFKALVFILIYEPIRKLTELYIRKIVFSYYYRRQEDLRDLDARLVSTMGYHEMADI
                     ITERISGLLNVKYAAIYLRIPEFFSLVSSYGRSDLITKRIKADMAVYDALLSGKRVFT
                     INELFVMHQTVLTDDALKVFQHEGCKYIIPMLKKDGISALLVLGEETQIRYDLSDEDK
                     KILWQSMQRVGNTLESTRLYSQLQKSLVEKEIVLEIAKKFNGEIHTEKLLDNILDAVK
                     SIVPYDAAGIFLVNEVTQEIESAVMRGYDPEVMEQIRMKVGTGLIGHVAIIGKPVIVK
                     DVNFDEHYVSVRDNTRSEITIPIKDSDRTIGVLNLESDMLGAYHEGFLDILNAIAGEA
                     GIAIQNAQLNEQAIRNEELQKELKIAGKIQQAILPQQLPRIPNLQITAKSLPCYAVGG
                     DFYDVLKLNDHQVTVSIGDVSGKGVPGAIMMSVLYTSYRGMVHEYKTSWEVVNSLNNL
                     LCTHTAEGRYATFFHCIIDFERLIMYYTNAGHNPPMIRKESGEWIKLREGGIVLGFIP
                     DQDYRQMTQPLDPGDIIVLYTDGITEVFNEAEELFGEERLKEVIENNRETSAKNIQDE
                     IIAAVSTFAPDQEMQQDDITLVIIKIEKSTLV"
     gene            2852..>3675
                     /locus_tag="PK754_13995"
     CDS             2852..>3675
                     /locus_tag="PK754_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB10661.1"
                     /translation="MASVDIKALNERIYIESGFIDNILKEVGLVIVGQKYMIERLLVA
                     LLSNGHVLLEGVPGLAKTLAIKTLSETIHTKFQRIQFTPDLLPADLLGTLIYNQKEGN
                     FYTRKGPVFSNLILADEINRSPAKVQSALLEAMQERQVTISDTTYPLPEPFLVLATQN
                     PVEQEGTYPLPEAQVDRFMLKLKVGYPTRDEELEIIRRNATTAPQLKVKPVATPEEIL
                     RARKTVNEIYMDEKIENYIVDLVFATRQPEKYGLPELKPLIHFGASPRASISLTLAS"
BASE COUNT         1027 a          871 c          794 g          983 t
ORIGIN      
        1 aacgatgata tcagcgattg taccgttggt acaccatagt ttttcaccat tgagaatgta
       61 cgactgaccg tcttcgcttt caacagcggt ggtgtgcaac ttggcgggat ccgagccggc
      121 ttccgtctcc gtcagcgcaa atgctgaaat ttcgcctttg gctaatcgag gcagatactt
      181 ttgtttttgt tcgtcggtac caaatagcat cagcggttga ggtacgccga tggattgatg
      241 tgcagataat aaggcaatca cattgccatc ttttgtacag atcaattcca ttattttatt
      301 atactcggct tgcgaaaatt ccaaaccacc gtattctttt ttgatcttca taccgaacgc
      361 acccatcgcc ttgagccggt cgcgcacggc catgggtatt tttccttcac gatcgatcgc
      421 atcggaatcc acttcctctt cgataaactt cttgaactcg gtataaaact gctgaaactc
      481 gggacggtta attcctttgg gttccggata cggatgaact aaatccaaac gaaggttacc
      541 taaaaaaatt tcacgcaaaa aactggggtt ttcccatttg ctctcgcggg cggattctgc
      601 tacttcccga gcctcttcct cggtaacatg gcgtttgccg ctttgtgcat catgagctga
      661 cattatcgga ttacctttcc tgtaataaca tatgtgataa ataaaaagct tgtttgtata
      721 aatcaaatat ccaaattcat tgcactaaat tcaaattaaa aataagccgc cagacggata
      781 ttacgcttgt atttttacgc gtggtcggct atattagccc gctttttaat tttcgttcaa
      841 atcttacgat ttcatgttca atttttctcg tacgctgctt cgtgatgtca tcttatatgc
      901 cgtcctcgtt gctattttta tcgccatttt tctcatcgga gaatatatct ccgacagtta
      961 ctttccgcgc accagcgact atgtgatttt tttcttcaaa gctctggttt tcatcctcat
     1021 ttatgaacct atccgtaaac tcacggaact ctatatccgg aaaatcgttt ttagctatta
     1081 ctaccgtcgc caggaagatc ttcgtgactt ggatgcccgg ttggtttcca cgatgggtta
     1141 tcatgaaatg gccgatatta tcaccgaacg gatcagcgga ttgctgaatg tcaaatatgc
     1201 cgcgatttac cttcgcatcc ctgaattttt ttcattggtg agttcgtatg gccgcagtga
     1261 tctcattacc aaacgtatta aagccgacat ggccgtttat gatgccttgt tatccggcaa
     1321 acgcgtattc acaataaatg aactgtttgt tatgcatcag accgttttaa ccgatgatgc
     1381 gttaaaagtt ttccaacacg aaggctgcaa atacatcata ccgatgttaa aaaaagacgg
     1441 cattagcgcc ttgcttgttt tgggtgaaga gacgcagatt cgctacgatc tgagcgacga
     1501 agataaaaaa atcctctggc aatccatgca acgcgtcggc aatacactgg aaagtacgcg
     1561 cctctacagc caacttcaaa aatcgctcgt agaaaaagaa atcgttctag agatcgccaa
     1621 aaaatttaac ggtgaaattc ataccgaaaa attgctcgat aatattctcg atgccgtcaa
     1681 atccatcgtg ccctacgatg ccgccggtat tttcctggtg aatgaagtga cgcaagaaat
     1741 cgaaagcgcg gtcatgcgcg gttacgatcc tgaagtgatg gaacaaattc gcatgaaagt
     1801 cggaaccggt ctgatcggtc acgttgcgat cattggcaaa cccgtcatcg taaaagacgt
     1861 caattttgat gaacactacg tcagtgttcg tgacaatact cgctccgaaa taactattcc
     1921 tatcaaagac agcgaccgca ccatcggtgt gctcaatctc gaaagtgaca tgcttggcgc
     1981 ctaccatgag ggatttctcg atatcctcaa tgccatcgcc ggcgaagccg gaatcgccat
     2041 acaaaatgca cagctcaacg agcaagccat tcgcaatgaa gaattgcaaa aagaacttaa
     2101 aattgcaggt aagatacagc aggctatcct cccgcagcag ttaccgcgta ttcccaatct
     2161 gcaaataacg gccaaaagcc ttccctgtta tgctgtcgga ggcgacttct atgatgtgct
     2221 aaagcttaat gatcaccagg tcacggtttc catcggcgat gtatccggaa aaggcgtacc
     2281 gggcgccatc atgatgtccg tactgtacac cagctatcgt ggcatggtgc acgaatacaa
     2341 aacgagctgg gaagtcgtta atagtttgaa caacctcctt tgcacacata cggccgaagg
     2401 ccggtatgca acgttctttc actgcataat tgattttgaa cgcctcatca tgtattatac
     2461 gaatgcggga cataatccgc cgatgatccg taaagaaagc ggtgaatgga tcaaacttcg
     2521 ggagggcggc attgttttgg gcttcatccc cgatcaggat tatcgtcaga tgacccagcc
     2581 gcttgatccg ggcgatatca tcgtattgta cactgacggg attacggaag tattcaacga
     2641 agccgaagaa ttgttcggcg aagagcgtct caaagaggtt atagaaaata accgtgaaac
     2701 ttctgctaaa aatattcagg acgaaatcat cgccgcggtt agcacatttg ccccggatca
     2761 ggagatgcaa caggacgata ttacactcgt gattattaaa atcgaaaaaa gtacattagt
     2821 ataaagatac agttttggag caagagacat catggcatcg gttgacatca aagcactgaa
     2881 cgagcgcatt tatatcgaaa gcggattcat tgacaacatt ctcaaagaag tcggcctcgt
     2941 tatcgtcggt caaaaatata tgatcgaacg tttattagta gcgcttttgt ccaacggcca
     3001 cgttctgctc gaaggcgtac cgggtcttgc caaaacctta gcgatcaaaa cattgtcgga
     3061 aacgattcac accaagtttc aacgtattca gttcacaccc gaccttttgc ccgcggattt
     3121 gctcggaacg ctgatttaca atcagaaaga aggaaatttt tatacccgca aaggaccggt
     3181 tttttccaat cttattttag cggatgaaat caaccgttca ccggccaaag tgcaaagcgc
     3241 attgcttgaa gctatgcaag agcgtcaggt taccatcagc gatacgacgt atcctctgcc
     3301 ggaacctttt ctcgtactgg cgacgcaaaa tcccgtcgag caagaaggaa catatcctct
     3361 gcccgaagcg caggtggatc gctttatgct caaactcaag gtgggttatc ctacgcgcga
     3421 tgaggaattg gagattatac gccgcaatgc aacgacggca ccgcaactga aagttaagcc
     3481 ggtggcgacc ccggaagaaa ttttacgcgc acgcaaaacg gtaaacgaaa tatacatgga
     3541 cgaaaaaatc gaaaactata ttgtcgatct cgtttttgcg acacgccaac cggaaaaata
     3601 tggattgccg gagttaaaac cgctgattca tttcggcgct tcaccgcgcg catccatcag
     3661 tttgaccctt gcatc
//
LOCUS       DAOQNQ010000313         3645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_12137_length_3645_cov_6.244011, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000313 DAOQNQ010000000
VERSION     DAOQNQ010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12137_length_3645_cov_6.244011"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PK754_14000"
     CDS             complement(<1..455)
                     /locus_tag="PK754_14000"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNB10662.1"
                     /translation="MGRILIGLIILSMQLLHAQQDTLFIRAGRLYDGIGSRIQKNKLL
                     VVFNDRIVRVDDDNRPSYNGKIIDLREKTVLPGLIDAHTHIVLHPGDYDGQILRETPE
                     YRAVYGTVLARKTLEAGVTTIRDLGNEGAGLADVALRDAIQKKIVPGPR"
     gene            671..1333
                     /gene="rpe"
                     /locus_tag="PK754_14005"
     CDS             671..1333
                     /gene="rpe"
                     /locus_tag="PK754_14005"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNB10663.1"
                     /translation="MPIIAPSILSADFANLSRDIRIAEESGADWIHVDVMDGRFVPNI
                     TIGPMVVEHLRKITKLPLDVHLMIVEPEKYITAFRDAGADIISVHQEACPHLHRTLQQ
                     IRQSGAKAGVVLNPSTSHDTLQYVLDDVDLILVMSVNPGFGGQKFIPQAAHKIRALRN
                     MLGSRKVFIEVDGGINNETGLQVIQAGADVLVAGSYVFGAENPKSRIQSLKQLHASTT
                     GV"
     gene            1403..2419
                     /locus_tag="PK754_14010"
     CDS             1403..2419
                     /locus_tag="PK754_14010"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNB10664.1"
                     /translation="MMPLPPFEFAAPATLDEAAKLLDQHKSHAKILAGGTDLIPSMKN
                     KLFEPQVLIGIGNIPEMKKIEYDPQKGLTLGSLVTITDIGHHPHITTHYPVLSDAARK
                     IATPLLQNKGTIGGNILLDTRCYYYNQSHFWRKAINNCLKKEGDVCRVAPSGHRCYAI
                     SSSDTVPVLYAMNASVTLVHAHGQRTVPLRAFYENDGMKFNKLEPGEILSQVHIPVLP
                     KGFRGAYMKLRRRQTIDYPMLGVMIGLQMDDKNICRNAQIVLGAVFSMPYEVKAAQDM
                     LIGQTITEDLIEKVADIAHQEAKPLDLTNFRPAYRKRMVRVFVKRILNVLTQKNTYVE
                     EILA"
     gene            2444..>3645
                     /gene="purD"
                     /locus_tag="PK754_14015"
     CDS             2444..>3645
                     /gene="purD"
                     /locus_tag="PK754_14015"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNB10665.1"
                     /translation="MNILVIGSGGREHALVWKIAQSPRVKKIYCANGNAGIRSLAECI
                     AIKPEDIHALADFAASHTIDLTVVGSETPLTLGIVDLFESRGLKIFGPSAKAAEIEGS
                     KVFSKQLMKKYGIPTAAFETFDNADQARSWIRSYDKPCVVKTDGLAAGKGAIVCMSTS
                     EALDAVDQIMSDKQFGSAGSQIVVEAFMEGEEASIFAVTDGTNYVVLPAAQDHKRALD
                     GDLGKNTGGMGAYAPAPVATHVVIERTKKEIIEPTLAAMAHEGRTYKGVLYCGIMVTP
                     DGPKVVEFNCRFGDPECQVLMMLISSDIVDLFQSVADNNLDPSAVTISDAHAACVVMA
                     SGGYPDAYAKGKIVNGLHTPLPENCAVFHAGTDWNENNIITNGGRVLGISATGADLKK
                     ALDTAYTVT"
BASE COUNT          991 a          878 c          806 g          970 t
ORIGIN      
        1 atccggggac cgggaacgat ctttttttgg atagcatcgc gcaacgccac atctgccaaa
       61 cctgcgcctt cgttgcccag atcgcgaatt gtcgttacgc ccgcctccaa agttttccgt
      121 gccaatacgg ttccgtaaac tgcgcggtat tccggagttt cacgcaatat ctgcccatca
      181 taatccccgg ggtgtaagac aatatgcgtg tgtgcgtcta tgagaccggg aagtaccgtt
      241 ttttcgcgta aatctatgat cttaccgtta tacgatgggc gattatcgtc atctacccgg
      301 acgattcgat cattaaaaac gacgagaagc ttattttttt gaatacgcga accgatcccg
      361 tcatacaaac gccccgctct gataaacagc gtatcctgtt gcgcatgcag caactgcata
      421 ctcaaaatga tcaacccgat taaaattcgt cccatgacaa attccctttc cgttatctaa
      481 aatagaatgg ttcggaaaaa acctttttac tcgtattttt ttgcctagta tgaagcgata
      541 tggaaaatat tcgatttata tttataacat ccaattcaaa tttgtcttat tactttcaca
      601 ccacttcccc gttgcttatc ccgcgcaata ttcataaaat agtttccgaa taattaactt
      661 tggtatattt atgcccatca tagcgccttc tattttatcg gccgattttg ccaacctttc
      721 gcgggatatt cgcatcgccg aagaatccgg tgcggattgg attcacgtcg atgtgatgga
      781 cggacgtttt gtacctaata ttacgatcgg tccgatggtc gtcgaacatc tccgtaaaat
      841 caccaaactt ccgctggatg ttcatcttat gatcgtcgaa cccgaaaaat atatcaccgc
      901 atttcgtgat gccggcgccg atatcatatc tgtacatcag gaagcgtgcc ctcatctgca
      961 tcgcacgctc cagcaaatac gccaatccgg cgctaaagcc ggcgtggtgc tcaatccgtc
     1021 cacttcgcac gacacgctgc agtacgtact tgatgatgtg gacttgatcc ttgttatgtc
     1081 cgttaatccg ggatttggcg gtcaaaaatt tattccccaa gcggcacata aaatacgcgc
     1141 attgcgaaat atgctgggct cacgtaaggt gtttatagaa gtggatggcg gaataaataa
     1201 tgaaacagga ttgcaagtta ttcaagccgg tgcggatgtg ctggtagccg gttcgtatgt
     1261 attcggtgca gaaaatccaa agtccagaat acaatcgcta aaacaattac acgcttcaac
     1321 aaccggcgtt taatttttga gccgatcaaa acaagtactt tattgttttt aaaaatatgt
     1381 tctacgctta agggtataat ttatgatgcc tttgcctccg tttgaatttg cggcgcccgc
     1441 gacattagat gaggccgcca aattattgga tcagcataaa tcgcatgcca aaattttggc
     1501 cggcggtacg gatctcattc cgagcatgaa aaacaaattg tttgaaccgc aagtgctgat
     1561 cggcatcggt aatattcccg agatgaaaaa aatcgagtac gatcctcaaa aaggactcac
     1621 tctcggcagt ttggtaacca tcacggatat cggccatcac ccgcatatca ccactcatta
     1681 tcccgttctt tccgatgcgg cacgtaaaat tgcaactccg cttttacaaa acaaaggcac
     1741 gatcggcggc aatattcttc tcgacacgcg ttgttattat tataatcaat cgcatttctg
     1801 gcgtaaggcc atcaataatt gtctgaaaaa agaaggcgat gtatgccgtg tcgctccgag
     1861 cggtcatcgc tgttatgcga tttcttcaag cgatactgta ccggtattgt atgcgatgaa
     1921 tgcgtcggta acactggtcc atgcgcacgg tcaacgcacc gtaccgctgc gcgcgtttta
     1981 tgaaaacgac ggaatgaagt ttaacaaatt ggaacccggc gaaatattat ctcaagtgca
     2041 tataccggtg ttaccaaaag gttttcgcgg tgcttacatg aagctgcgcc gccgacagac
     2101 cattgactac cccatgctcg gtgttatgat cggtttgcaa atggatgata aaaatatttg
     2161 ccgcaatgcg cagattgttt taggcgccgt attttctatg ccctacgaag tcaaagccgc
     2221 gcaggatatg ctcatcggtc aaaccattac tgaagacctg atcgaaaaag tcgctgacat
     2281 cgcacaccag gaggccaaac ctctggattt gaccaatttc cgaccggcct atcgaaaacg
     2341 tatggttcgc gtttttgtta aacgtatttt gaatgttttg acacaaaaaa acacctacgt
     2401 ggaagaaatt ttagcttagt tttttgaatc acatataaga tctatgaata ttctcgttat
     2461 agggtccggc ggacgtgaac atgctttggt ttggaaaatc gcccaatcgc cgcgtgttaa
     2521 aaaaatctac tgtgccaacg gcaacgccgg catacgatcg ttagccgaat gtattgccat
     2581 taagccggaa gacattcatg cactcgctga ttttgccgca tctcatacaa ttgatttgac
     2641 tgtcgtcggt tcagaaactc cgctcacttt gggtatcgtt gatctttttg aatcacgcgg
     2701 acttaagatt ttcggaccct ccgccaaagc ggccgaaatc gaaggcagta aagtcttttc
     2761 caaacaattg atgaaaaaat acggtattcc taccgcagcc tttgaaacat ttgataacgc
     2821 cgatcaagcg agatcatgga tacgatctta cgacaaaccc tgcgtcgtaa aaacagatgg
     2881 attagcggcc gggaaaggcg ctatcgtctg catgagcacg tccgaagcgc ttgacgcggt
     2941 ggatcaaatc atgtcggaca aacaatttgg ttccgccgga tcacaaatcg tcgttgaagc
     3001 atttatggag ggtgaagaag cttctatttt tgccgtaacg gatggaacga attacgtcgt
     3061 acttccggcc gcacaagacc ataaacgcgc actggacgga gatctcggaa aaaataccgg
     3121 cggtatgggc gcctacgcac cggcacccgt cgccacccat gtggtgatcg aacgtaccaa
     3181 aaaagaaatt atcgaaccca cactggccgc tatggcccat gaaggtcgaa cgtataaagg
     3241 tgtgttatat tgcggcatca tggtgactcc cgatggtccc aaagtagtcg agtttaattg
     3301 ccgttttggc gatcccgaat gccaagtttt gatgatgctt atatcgtccg atatcgtcga
     3361 tcttttccag tctgtcgcgg ataacaatct tgatccgtcc gccgtaacca taagcgatgc
     3421 ccatgcagct tgtgtcgtaa tggcctccgg cggctacccg gacgcgtacg ccaaaggaaa
     3481 aatcgtcaac ggattacata cgccattacc tgaaaattgt gcggtatttc atgcgggaac
     3541 ggattggaat gaaaacaaca ttatcaccaa tggcggcaga gtcttaggta tttccgctac
     3601 aggcgccgat ctaaaaaaag ctttggatac cgcctatacc gtaac
//
LOCUS       DAOQNQ010000314         3568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_12558_length_3568_cov_4.373755, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000314 DAOQNQ010000000
VERSION     DAOQNQ010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12558_length_3568_cov_4.373755"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="PK754_14020"
     CDS             complement(<1..1197)
                     /locus_tag="PK754_14020"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB10666.1"
                     /translation="MVCGYVAHGADGKPQRHFQVRSYTLKDGLSQNLVTCALQTRDGF
                     MWFGTREGLNRFDGYEFLVFQHDESDSFSISHNYVTALLEDQDGRIWVGTEDGLNLFD
                     PITGRFEKFFHDPMQPASISNNNINVLFQDSFGTLWIGTHVGLNKLRQSDEPNFERVL
                     TISENKTSLPSNLIRAIGQVHNAGGSAEKKLWIGTTGGGLSVLDMETNTFSKPYDDLI
                     VSKKFNMTTVWDVYEDPADRGNTLWVGTPSGLGKIDRATRQLHFFSFDNKNPNGISHN
                     RVRRILRDSYGRLWVGTTEGGINLMTDDTFLNFRNTPNQPSLFTDEYIYDIYQSSDGI
                     VWICTYSNGVTQVSGMEPRFVHYKKNPIDPASLIHNVVRSFTESRDGSIWISTDDGIS
                     RWDRTSD"
     gene            complement(1354..1827)
                     /locus_tag="PK754_14025"
     CDS             complement(1354..1827)
                     /locus_tag="PK754_14025"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HNB10667.1"
                     /translation="MQTEGRNAIHRQWLFLNKLPLGSWIFSYFVNRYIPYTGSISARV
                     LELKPGYARVLLRDRRKVRNHLRSIHAIALANLAEYTGNLALVCGLPEDARFIVKNIA
                     VDYQKKARGTLVGECQTEIVATAETRDISVRVDIRDQDGDTVATGLLLTRIGPKK"
     gene            1967..3556
                     /locus_tag="PK754_14030"
     CDS             1967..3556
                     /locus_tag="PK754_14030"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNB10668.1"
                     /translation="MKKIDFLNWSLDAWFLALCLMLCVTCMAFSCSLNAQEKDTVVLR
                     DSAATEGDSVDTFNATVQNVVNETRRIVKRRAQAASRFLNERYIIVIDPGHGGTKYID
                     KGNSAKLDGIEYHEKDMMFEYAQLVEKKLAAAGYEGLRKTKEAADDSSLSIYTRAGRA
                     AQFGKNAGKKVLFVSLHWNNFEDPSVGGTEIYIKESQNYKSRKLAEFLRGSMSHILQM
                     HGAGQNITGIVERDYRVLREMDTGVLIEIGYASNPGDLRKMLSQKDEIATAIVNGIDA
                     FAEYMQEQEAQKQMPNDDPKKAAEDAKLAEIPSIPFGWSWRKKDISADVDHLMKRYDG
                     MYEKVTTEKEIYLTFDCGWENGYTPKILDVLKANEVKAAFFITGGYMKSAEDVVARMV
                     QEGHIVGNHSVNHLSMPQLKVREMKREILGLEQDFYDLFEMKMNYFRPPSGEWSRRSL
                     AVTQALGYKTVFWSFAYDDWDVQAQHGRDYAYKKITQNVRPGVIILLHAVSKDNYEAL
                     DAVIKDLRKDGYVFKSLNEIK"
BASE COUNT         1032 a          772 c          850 g          914 t
ORIGIN      
        1 atcagatgtt ctatcccaac gcgaaatgcc gtcatcggtg ctgatccaga tcgaaccatc
       61 gcgactttcc gtgaaactgc gtaccacatt atgtatcaag ctggcggggt ctatcggatt
      121 ttttttgtag tgaacaaatc gcggttccat gccgctaacc tgcgttacgc cgttggaata
      181 cgtacaaatc cagacgatac cgtcggaaga ttgatatatg tcgtatatat actcatcagt
      241 aaaaagcgat ggctgattgg gtgtatttct gaagttgagg aatgtatcat cagtcatcaa
      301 attgatgccg ccttcggtcg taccaaccca caagcgtcca tacgagtcgc gcagaatgcg
      361 gcgcacacga ttatggctta taccgttcgg gtttttgtta tcaaaagaaa aaaaatgcaa
      421 ttgccttgtc gcccggtcta tttttcctaa acctgacggc gtacctaccc acaaggtgtt
      481 gccccggtct gccggatctt catacacatc ccaaactgtg gtcatattga atttcttcga
      541 tacgatcaga tcgtcataag gttttgaaaa tgtattggtc tccatatcca atacactgag
      601 accgccgccg gtggtaccga tccaaagttt tttttctgcc gagccacctg cattatgaac
      661 ctgaccaatc gcccgaatta aattggacgg taaacttgtt ttgttctcgg aaatggttaa
      721 aactcgttca aaattcggtt catccgactg gcgtaattta ttaagtccga cgtgggtacc
      781 gatccacaat gtcccaaaac tatcctgaaa caacacattg atattattat tgctgatgga
      841 tgccggttgc atcgggtcat gaaaaaactt ttcgaagcgg cctgtgatgg ggtcaaaaag
      901 gttgagtccg tcttccgttc cgacccaaat acgtccgtct tgatcttcga gcaaagccgt
      961 tacataattg tgagaaatgg aaaagctgtc actttcgtcg tgctgaaaaa caaggaactc
     1021 atacccgtca aagcgattga ggccttcgcg tgtaccaaac cacataaatc cgtcacgagt
     1081 ctgaagcgcg caggttacca gattttgaga taatccatct ttgagcgtat aggatcgcac
     1141 ctggaaatgg cgttgtggtt taccgtcagc gccgtgggcg acgtaaccac aaaccataat
     1201 ccatatgcac aataccgtta caaaacgacg ccgtgggaac atgtgaaaaa agattaatga
     1261 aatgagatcg gtgtagtgaa acctaatcga aaagccgttg aaaaaaaagt gaaaaatatt
     1321 atttaataca tgcctgattt ggaacgtcaa aatttatttt ttagggccga tgcgcgtaag
     1381 taataaaccg gtggctacgg tgtctccgtc ttggtcgcgg atatcaaccc gcacggagat
     1441 atcgcgcgtt tcggctgtcg ccacgatctc ggtttggcat tcgcccacaa gcgtgccccg
     1501 agcttttttt tgataatcca ccgcgatatt ttttacgata aaacgcgcat cctccggtaa
     1561 tccgcatacc aatgcaagat tgccggtgta ttccgcgagg ttggcaagag cgatggcgtg
     1621 tatcgaacgc agatggttgc gcactttacg ccgatcccgt aataaaacac gcgcataacc
     1681 gggtttgagt tccaaaacgc gggctgatat gctcccggtg taaggtatgt agcgattgac
     1741 aaaataactg aatatccagg aacccaatgg aagtttattt aaaaataacc actggcggtg
     1801 tatagcgttg cgtccttcag tttgcataaa acattatcag ggtttttatg aaaaatacaa
     1861 tttcattccg ttcatacgct tattgtaaag gaaatccgac taaattccaa ttgaattgtg
     1921 tctgtgaaaa gactacttta cgcgcgaatt taaagaaaat gtatatatga agaaaatcga
     1981 ttttttaaac tggtcacttg acgcttggtt tttagcgtta tgtttgatgt tgtgtgtaac
     2041 atgtatggct ttcagttgtt cattgaacgc acaggaaaag gacaccgttg ttttgcgcga
     2101 ctccgctgca accgagggcg acagtgtgga cacgtttaat gccaccgtac agaatgtggt
     2161 gaatgaaaca cgacgtatcg tcaaacgacg cgcccaagcc gcatcgcgtt tcctgaacga
     2221 acgttatatc atcgtcatag atccgggcca tggcggcaca aagtacatag acaaagggaa
     2281 ttcggccaaa ttggatggca tcgaatacca cgaaaaagac atgatgtttg aatatgcaca
     2341 gctcgtggag aaaaaattag ctgcggcagg atatgaagga ctgcgaaaaa cgaaagaagc
     2401 ggcggatgat tcgtcgctat ccatttacac gcgtgccggt cgtgcggccc aattcggaaa
     2461 aaatgcgggc aaaaaagttc tttttgtcag cctgcactgg aataattttg aagatccttc
     2521 ggtcggcggt acggagatat atatcaaaga aagtcaaaat tacaaaagcc gtaaattggc
     2581 agaatttctg cgcggttcga tgagtcatat tttacaaatg cacggtgccg gtcaaaatat
     2641 tacgggtatc gtcgaacgcg attatcgcgt actgcgcgaa atggataccg gcgttttgat
     2701 cgaaatcggg tacgcaagca accccggcga cctccgaaag atgctgtcac aaaaagacga
     2761 gattgccacc gctattgtca atggcattga tgcttttgcg gaatatatgc aggaacaaga
     2821 ggcgcaaaaa caaatgccca atgatgatcc caaaaaagcg gcagaggatg ccaagttagc
     2881 cgaaattccc agtattccgt ttggctggtc ctggcgcaaa aaagatatta gcgccgatgt
     2941 ggatcatctg atgaaacgct acgatgggat gtacgaaaaa gtcactactg aaaaagaaat
     3001 ttatctgacg tttgactgcg gttgggaaaa cggatatacg cccaaaatat tagatgtgct
     3061 gaaagccaac gaggtcaagg ccgcattttt cattaccggc ggatacatga agtcggcgga
     3121 ggatgtcgtg gcacgtatgg ttcaggaagg tcatatcgtc ggtaatcatt ccgttaacca
     3181 cctcagcatg ccacagctca aagtgcgcga gatgaaacgc gagattcttg gtttagaaca
     3241 ggatttttat gatctttttg aaatgaaaat gaattatttc cgtccgccca gcggcgaatg
     3301 gagtcgtcgt tcgctggcgg tgacccaggc gctgggatat aagaccgtat tttggtcgtt
     3361 tgcgtacgat gattgggatg tacaggcaca acacgggcgg gattatgcat acaaaaaaat
     3421 cacccaaaat gttcgccccg gcgtgattat tctgttacat gcggtatcaa aggataatta
     3481 tgaggcactc gacgccgtga tcaaagatct gcgcaaagac ggctatgtat ttaaaagctt
     3541 gaacgaaatc aaataaggaa taaggacg
//
LOCUS       DAOQNQ010000315         3535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_12771_length_3535_cov_5.644828, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000315 DAOQNQ010000000
VERSION     DAOQNQ010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12771_length_3535_cov_5.644828"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1913)
                     /locus_tag="PK754_14035"
     CDS             complement(<1..1913)
                     /locus_tag="PK754_14035"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNB10669.1"
                     /translation="MKLNRLTILILFLPIFTIFSQFANSGSNAFQQSVRYEIDLNLNI
                     SENIIVAEEKLFYRNNSPDTLEYVWFHLYPNAYKNENSIFAREMAERGVNDFFYTSEK
                     DRGYIEIKKISVNNLALTTRFKENDETELKVNLDKSLSPGDSVIFEIQFKVKIPSFIS
                     RMGQKDLRYEITQWYPKIAVYDNNGWHNDGYHFLGEFYGEYGDYKVSFNVPEWMWVAA
                     TGENIDKDEIAKINDMVLFTSKIDSLPKKLKRLKLDSIVTVQKEIKKCLLAGKFKNKK
                     ITFVAQNVHDFAWACDYRYLIKESFYENTKILTYVTPKHYIGWEYSNDYASDALRYMS
                     NWYGDYIYPVLNVVESNGPYGGMEYPNLVLITSRGDGLLRTFEEVIFHEIAHQWFYGM
                     LGNNEINDAWLDEGLTMFSEMRYFREKYKGSFSNSKFLNLLGLDQITEHSELMKANLY
                     FISGFDYIPPASEPSPNYRSRLEFSNVYVRTAFTLDMLKYELGDSLFNTCMKEYYKRF
                     LLKHVSPASLINTFNDVTSSSWDWYFRQWIYTQEQLNLKAGKLESYSQSENQFETSFK
                     IIKESKLKTNCKIEFTTKQNERFVERIFIEQPETEIRVNSKDEIISARIDPHNELLEF
                     NRRDNSTNYSGFRL"
     gene            complement(1989..2111)
                     /locus_tag="PK754_14040"
     CDS             complement(1989..2111)
                     /locus_tag="PK754_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10670.1"
                     /translation="MEIKAEKLETKNTTELSVADYLILGGGLMIAAGYVYNQVS"
     gene            complement(2137..2430)
                     /locus_tag="PK754_14045"
     CDS             complement(2137..2430)
                     /locus_tag="PK754_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10671.1"
                     /translation="MVEAVINLFSKKWNKKRKKNITKFRAGSLRIKSNIFFQLQCIQL
                     NKNTQCFVLKLEDFMEIKAEKLETKNPTEMSVGDWLILIGAGMMVAGGFMQQS"
     gene            complement(2709..2894)
                     /locus_tag="PK754_14050"
     CDS             complement(2709..2894)
                     /locus_tag="PK754_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10672.1"
                     /translation="MKKTLGQIAMHVTHLDVMEDFYKNTVGLTHLFTVPNRLSFFDLG
                     GVERPKNILFDFMCVIY"
     gene            complement(2903..>3535)
                     /locus_tag="PK754_14055"
     CDS             complement(2903..>3535)
                     /locus_tag="PK754_14055"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNB10673.1"
                     /translation="NLAEAPGHLSDEEAAALPLAGLTAYRALMKQGKADAGETVVITG
                     IGGGVAALTLQMAMTVGAQVVVTSGHDEKIARALSLGASAGYNYKATDMFKKMASDGI
                     EPDLIVDGTGGASFGEWLNLMKPGGRYVTYGATNGNPEHIDLRKIFWKQLTIQGSTMG
                     TIDDFRAMIKIYDQYRLKPAVDKVFSLAECRSAFHRMESSEQFGKIILKP"
BASE COUNT         1055 a          727 c          572 g         1181 t
ORIGIN      
        1 agcctaaatc cagaataatt agtagagtta tctctccgat taaattctaa aagttcatta
       61 tgaggatcaa tccttgcgct aataatttca tcttttgaat tcacacgaat ttcagtttcc
      121 ggttgctcaa taaatattct ttcaacaaat ctttcatttt gtttagttgt aaactctatt
      181 ttacaattgg ttttcaattt actttctttg ataattttaa aagaggtttc gaattgattt
      241 tctgattgag agtatgattc tagtttcccc gcttttaggt taagttgctc ctgggtataa
      301 atccattgcc taaaatacca gtcccaacta gaagaagtca catcattaaa tgtgtttatt
      361 aacgaggctg ggcttacatg ttttagcaag aatcgtttat aatattcttt catgcacgta
      421 ttaaaaaggc tgtcgcctaa ttcatacttt aacatatcta aagtaaatgc cgtacgcaca
      481 taaacattac taaattccaa tctgcttctg taattaggtg aaggttcaga tgcaggagga
      541 atatagtcga aaccggatat gaaatataaa ttcgctttca ttagctcaga atgctctgtg
      601 atctgatcaa gacctaataa attcaggaat ttcgagttac tgaatgaacc tttatatttt
      661 tctctaaaat aacgcatttc tgaaaacatg gtcaacccct catcaagcca agcgtcattg
      721 atttcattgt tacccaacat tccataaaac cattggtgtg caatttcatg aaatatcact
      781 tcctcaaagg ttcgcaataa gccatcacca cgagaagtga ttaaaactaa gttaggatac
      841 tccattccac cataaggtcc attgctttcc acaacattca atacagggta aatataatca
      901 ccataccagt ttgacatgta tcggagcgcg tcagatgcat agtcatttga atattcccaa
      961 ccgatgtaat gtttgggcgt tacgtaggtt aatatttttg tattttcata gaacgattct
     1021 ttaattaaat aacgataatc gcaagcccaa gcgaaatcat gcacattttg cgctacaaat
     1081 gttattttct tgtttttaaa tttaccagct aacaaacatt tttttatttc cttttgtacc
     1141 gtaacgatgg aatcaagttt taaccgttta agttttttgg gaagtgaatc tatcttagag
     1201 gtaaacaaca ccatatcgtt tattttagca atttcatctt tgtcaatatt ttctcctgta
     1261 gcggcaaccc acatccattc tggtacattg aatgaaactt tgtaatcacc gtattccccg
     1321 taaaattcgc cgagaaaatg atagccatcg ttatgccacc cattattatc ataaactgca
     1381 attttggggt accactgagt aatctcatat cgcagatctt tttgccccat acgagatata
     1441 aaactgggga ttttgacttt gaattgtatt tcaaaaataa ctgaatctcc gggggataga
     1501 gatttatcta aatttacttt taactctgtt tcatcattct ccttaaatct tgttgttaaa
     1561 gcgagattat tcacggaaat tttttttatt tcaatatatc ctctatcttt ttctgaagta
     1621 taaaaaaaat cgttaactcc ccgctctgcc atttctcttg caaagataga attctcgttt
     1681 ttgtatgcat ttgggtaaag gtgaaaccat acgtactcta atgtgtcggg tgaattgtta
     1741 cgataaaata acttttcttc agctacgatg atgttttcag aaatatttaa atttaaatct
     1801 atttcatatc ttacactttg ttgaaaagcg ttacttccac tatttgcaaa ttgtgaaaat
     1861 attgtaaaaa tcggaagaaa caatatgagg atagtcaacc tatttaattt catggtattt
     1921 acgttgcttt tcaaataaag caaggcaggt attgttaaaa caacacctgc cgttgcgttt
     1981 agaagtgact aacttacttg gttatataca tagccagccg ctatcatcaa acctccacct
     2041 aaaataagat aatcggcaac tgataattca gttgtatttt ttgtttcgag tttttcagct
     2101 ttaatttcca tgatacaaat ttcctttagt ttaagtttaa ctttgttgca tgaaaccacc
     2161 ggcaaccatc atgccggcac caatcaaaat caaccaatca ccaacagaca tttccgtggg
     2221 attttttgtt tctaatttct cagctttgat ttccataaaa tcctccagtt taagaacaaa
     2281 acattgcgta ttcttattta attgaataca ttgcaattgg aagaaaatat tactttttat
     2341 tcttaagctc ccagctctga atttggtaat atttttcttc ctttttttgt tccatttttt
     2401 tgaaaataag ttaattactg cttcaaccat tgttcaggat gaatttactc acctccttta
     2461 tataaataaa acatgaattc cttccaacgc caaactgata ttggattgga ttggattgga
     2521 ttggattgga ttggattgga ttggattgga ttggattgct actaactaag attcacaacc
     2581 cccattttcc cttatagaac agtttaatat agacacacca gtccctgatg tcaataagat
     2641 tttgtgttcg ttctaatata tcacgacaat cttctaatca agccactagt cccccgtact
     2701 tatcctattc aatagattac acacataaaa tcaaacaata tattcttagg tcgctcaaca
     2761 ccgccaagat caaaaaaaga taaccggttc ggaacagtaa acagatgcgt cagacccacc
     2821 gtatttttgt aaaaatcttc cattacgtcc agatgggtca catgcattgc gatttgaccg
     2881 agcgtttttt tcatacaaaa ctttacggtt taaggataat tttaccaaac tgttcacttg
     2941 attccatacg atgaaatgcg gaacggcatt cggctaaaga aaacacttta tccacagccg
     3001 gcttcagtcg gtattgatcg tagatcttaa tcatagctct aaaatcatcg atagttccca
     3061 tcgtggagcc ttgtatagtc agttgtttcc aaaaaatttt tcgcaaatct atgtgttccg
     3121 gattaccgtt agttgcgcca tacgtcacat aacggccgcc cggtttcatg agattgagcc
     3181 attcgccaaa tgatgcgcct ccggtgccat ccacgatgag atcgggttcg ataccgtccg
     3241 aagccatctt tttgaacata tctgttgctt tatagttata accggcggat gcaccgagcg
     3301 aaagtgcgcg tgcgattttt tcatcatgac cggacgtcac gacgacctgc gcaccgacgg
     3361 tcattgccat ttgcagagtc aatgccgcta cgccgccgcc gatgcccgtg atcacgaccg
     3421 tttcgcccgc atcggcttta ccctgtttca taagcgcacg atacgccgtc aaaccagcca
     3481 gcggcaacgc cgccgcttct tcatccgaaa gatgccccgg cgcttcggct aaatt
//
LOCUS       DAOQNQ010000316         3524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_12848_length_3524_cov_5.949553, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000316 DAOQNQ010000000
VERSION     DAOQNQ010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12848_length_3524_cov_5.949553"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PK754_14060"
     CDS             complement(<1..378)
                     /locus_tag="PK754_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10674.1"
                     /translation="MKSLPMLRIPIVRYYSLHLHTHAPHDTVPYPDLYASFAQTAYSQ
                     MILSDVAFDLKTMQKQTRNTALDEFQVIENYTAKLDDHSTINITIYEAYDLPKDLLIA
                     DLSEELLETLEIIYLFYKGAIFPG"
     gene            535..1563
                     /gene="cheB"
                     /locus_tag="PK754_14065"
     CDS             535..1563
                     /gene="cheB"
                     /locus_tag="PK754_14065"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HNB10675.1"
                     /translation="MSQIRVLIADDSPTAVDIIATILETDDDIRIVGKAVNGREAVAM
                     AETLRPDVITVDIQMPIMNGLDAIKQIMAHYPTPILVITSSTDASIAFQALSFGALEV
                     VEKPSLELDFHARKYEEFIRKVKLIARVKVITHISGKMVDVKTVSDTSDFLLVIGIVA
                     STGGPRALASILGSLPKNLSAPVLVVQHIADGFTDGLIQWLADVSPLRVKSAEQGESL
                     ARGTVYIAPNGKHLEIGAQNKIVLSEKEDVSGHKPSGSVLLRSLATHAKRHAVGVLLT
                     GMGRDGADGMLAIKQNGGHTIVQDEATSLIFGMPKAAIDIGAAEIVLPAERIAEQITR
                     WTGIKTDA"
     gene            1573..3036
                     /locus_tag="PK754_14070"
     CDS             1573..3036
                     /locus_tag="PK754_14070"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNB10676.1"
                     /translation="MQQEFTRALFETFRKTILDFCGIAIPDDSRSELERKIRERMTQT
                     GFSDFYSYHVHIAGREAGEDELRKFIESITNNETYFFREPAHFRVLKSHIVPELLRKK
                     KERRIRVWSAGCSTGEEAYTIAISLFETRMLLGDFEIEVLATDIDRNALTIAQLGNFG
                     RNSFRAIDPSYLNTYFRIPDQRLMDYRIIDERIRAITRFEYLNLFDDHYPDYLHDIDV
                     IFFRNVSIYFPKDKIQSVNRRLSEVLNESGYLFLGSSETLHHNFGHLDLVDYDGVFLY
                     QKKTGTEEKSKKTIAPSLHESKRHHRPVSPKESAKPQTPRSASSRTKPEAPPSLDQIF
                     AMYKDGAYAQILALWDMHADGLEPKIPFLILKAFIYIGQEDFDLARKTCDAMMALDAL
                     NAEVYFLSGLVDYHAGQFAAATEKLKKALFLKKDLAMAHYYLASVYRQSGKTDDARRE
                     FRNTIKILEHKQDTSVSFLAQGYSSDYLINACKQYLK"
     gene            3033..>3524
                     /locus_tag="PK754_14075"
     CDS             3033..>3524
                     /locus_tag="PK754_14075"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNB10677.1"
                     /translation="MNTRFDRSTFIQKFVLETRENIRRLNNALLRIEKNPGDTDAPDE
                     ILRTLHTIKGSARMLNLRAISELMHAFEEAFGNAHKQKYMHERAVMDTFLRTLDKTDT
                     HLDDIVRDGHSEADLSSWLHRITLIAERKINELQKEASADTSDKKITETTEPVAEPVT
                     PKKS"
BASE COUNT          927 a          844 c          889 g          864 t
ORIGIN      
        1 gcccgggaaa atagcgcctt tataaaaaag ataaatgatt tccagtgttt ccaaaagctc
       61 ttcggataaa tcggcgatca aaagatcttt gggcagatcg taggcttcgt agatggtgat
      121 attgatcgtc gaatggtcat ctaatttggc cgtataattt tcgatcacct gaaattcgtc
      181 caacgccgta ttgcgcgttt gcttttgcat cgtttttaga tcaaaagcga catcgctgag
      241 gatcatttga gaatacgcgg tctgcgcaaa tgacgcgtac aagtcggggt atggtaccgt
      301 atcgtgcggt gcatgcgtat gcaagtgcag actatagtag cgaacgatgg gaatgcgtaa
      361 catgggcaaa gatttcatgc gcccaaaata ggcacgatac cggcgtaatg caagaatttt
      421 aaccggatgc ggaaaacagc gcgatatttg tagactaaac cttgtatatg caggccgtct
      481 gatatatatt ttcaccatat tcaaaacgcg ttctcaccta ccttttttat tcgtatgagt
      541 cagattcgtg ttctgattgc cgatgattcg ccgaccgccg tggacattat cgccacgatt
      601 ttggaaaccg atgacgatat ccgtattgtc ggtaaagcgg tcaatggccg cgaagccgtg
      661 gctatggccg aaacgctgcg cccggatgtg atcacggtgg atatccagat gccgatcatg
      721 aacggccttg atgcgatcaa acaaatcatg gcccactatc cgacgccgat tttggtgatc
      781 acctccagta cggatgcgag cattgcgttt caggcgttat cgtttggcgc gctggaagtg
      841 gtcgagaagc cctccctcga attggatttt catgcgcgta aatacgaaga atttattcgc
      901 aaagtaaaat tgatcgcacg ggtcaaagtg attacgcata tctcaggcaa gatggtggat
      961 gtcaaaaccg tcagcgatac gagtgatttt ttgcttgtca tcggcatcgt tgcgtccacc
     1021 ggcggcccgc gtgcgctggc ttcgatactg ggttctttgc ccaaaaacct ttccgcgccg
     1081 gtattggtcg tgcagcatat agccgacggg tttaccgacg gtctgatcca gtggcttgcc
     1141 gacgtgtcgc cgcttcgggt caagtccgcc gaacagggtg aatcgttggc acgcggtacg
     1201 gtatatatcg ctcccaacgg taaacacctg gaaatcggag cgcaaaacaa aatcgttctg
     1261 agtgaaaaag aagacgtatc cgggcacaaa ccgtcgggtt cggttctttt gcgttcgctg
     1321 gcgacccatg ccaagcggca tgcggtgggc gtacttttga ccggtatggg ccgggacggc
     1381 gccgacggta tgctggctat aaaacaaaac ggcggacata cgatcgtaca agacgaagcg
     1441 acttcgctga ttttcggtat gcccaaagcc gcgatagaca taggcgccgc ggaaatcgtt
     1501 ttaccggcgg agcgtatcgc cgaacaaata acccgttgga cgggtatcaa aaccgacgcc
     1561 tgaggataac cgttgcaaca ggaatttacc cgcgcattat ttgaaacgtt taggaaaacg
     1621 atcctcgatt tctgcggaat cgccataccc gatgacagcc gcagcgaatt ggaacgtaaa
     1681 atccgtgagc gcatgacgca gaccggattt agtgattttt attcctacca cgtacacatc
     1741 gcaggccggg aagccggcga agatgaattg cgtaaattca ttgaatccat caccaataat
     1801 gaaacgtatt tttttcgcga gccggcccat tttcgcgtac tcaagtctca catcgtgccg
     1861 gagttgcttc gtaaaaagaa agagcgccgg attcgcgtgt ggagcgcggg ctgctcgacc
     1921 ggcgaagaag cttataccat cgccatcagc cttttcgaaa cgcgcatgtt gctcggtgat
     1981 tttgagatcg aagtgctggc aaccgatatt gatcgcaacg cgttgacgat agcgcagctc
     2041 ggtaattttg gccgcaattc tttccgcgcg atagacccgt cgtatcttaa cacctatttt
     2101 cgcatccctg atcaacggct tatggattac cggatcatag acgagcgcat tcgcgccata
     2161 acgcgttttg aatatctcaa tttgtttgac gatcattatc cggactatct gcacgatatt
     2221 gatgtgatct ttttccgcaa cgtatccatt tattttccca aagataaaat ccaatcggtg
     2281 aatcgccgct tgagcgaagt gctcaatgaa tcgggatatc tgtttttggg ttcatccgag
     2341 acgttgcatc ataatttcgg tcatttggat ctggtggatt acgacggtgt ctttttgtat
     2401 cagaaaaaaa cgggaaccga agaaaaatcc aaaaaaacga tagcgccttc gcttcacgaa
     2461 tcgaaacgtc accatcgccc ggtttctccg aaagaaagcg ccaaaccgca aacgccccga
     2521 tccgcgtcgt cacgcaccaa accggaagcg ccgcccagtt tggatcagat ctttgcaatg
     2581 tacaaagacg gcgcgtatgc acagattctg gcattatggg acatgcacgc tgacgggcta
     2641 gaacctaaaa ttcctttttt gattttaaaa gctttcatct atattgggca ggaagatttc
     2701 gatttggcgc gcaaaacctg cgatgcgatg atggcgcttg atgcgctcaa tgcggaggtt
     2761 tattttttaa gcgggctggt agattaccat gccggacaat ttgccgccgc aacggaaaaa
     2821 ttgaaaaaag cgctgttttt gaaaaaagac ctcgcgatgg cgcattatta tttggcttcg
     2881 gtatatcgcc aaagcgggaa aacggacgat gcgcggcgtg aatttcgcaa caccatcaaa
     2941 attttagaac ataaacagga tacttcggtg tcttttttgg cgcaggggta ttcgtccgat
     3001 tatctgatca atgcttgtaa acaatatttg aaatgaacac gcgcttcgat cgcagcacat
     3061 ttattcaaaa gtttgtactc gaaacacggg aaaatatccg gcgtttgaac aatgcgttgc
     3121 tccgtatcga aaaaaatccg ggcgataccg atgcaccgga tgaaatcctg cgcacgctcc
     3181 ataccatcaa aggttcggcg cgtatgctca atctgcgcgc gatcagcgag ctgatgcatg
     3241 cgtttgagga ggcgttcggt aatgcgcaca aacaaaagta catgcatgaa cgtgccgtga
     3301 tggatacgtt tttgcgcacg cttgataaga cggatacgca tctggacgat atcgttcgcg
     3361 acggacattc cgaagccgat ctttcgtcgt ggcttcatcg catcacgctc atcgccgaac
     3421 gaaaaatcaa cgaactgcaa aaagaagcaa gcgccgacac atcggataaa aaaatcaccg
     3481 agacgacgga accggtcgcc gagccggtca cgcccaaaaa atcg
//
LOCUS       DAOQNQ010000317         3515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_12907_length_3515_cov_5.423988, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000317 DAOQNQ010000000
VERSION     DAOQNQ010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12907_length_3515_cov_5.423988"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PK754_14080"
     CDS             complement(<1..413)
                     /locus_tag="PK754_14080"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HNB10678.1"
                     /translation="MKKILLIHTGGTMGMKFGYPAPLPHEQFADEVFRHIPGVRDIAD
                     VDFFSSFSMDSSNLSPEHWVTLGRIIWDNMDAYDGFVIIHGTDTMSYTASALSYMLSN
                     LAKPVILTGSQRPLSAIRTDAKNNLINAIELATYP"
     gene            631..822
                     /gene="rpmB"
                     /locus_tag="PK754_14085"
     CDS             631..822
                     /gene="rpmB"
                     /locus_tag="PK754_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNB10679.1"
                     /translation="MKRCDVCGKSPMYGNNVSHANNRTKRRWLPNLQEVRAKMNNGAV
                     KRVKVCTQCIKSGKVAKAA"
     gene            1066..>1646
                     /locus_tag="PK754_14090"
     CDS             1066..>1646
                     /locus_tag="PK754_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10680.1"
                     /translation="MYRRLFLLVWCAPILAFAQVFVPATRMPKTWINTLSLSSGFEYN
                     TWSPYKADELRYTIEGFQIGYLRGEIDHNVPMLPNLNFSWETNFGGKKQNELMQAQNS
                     TSKLEQGYEKLHTALEFGKKKIYNDAGQVINTMANWELTYTKETFYIAVSPKSSDLRY
                     AAYYSDAVYNFPQGTKYSMFTKFQEIGLTAYTGG"
     assembly_gap    1647..1746
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1747..2022
                     /locus_tag="PK754_14095"
     CDS             <1747..2022
                     /locus_tag="PK754_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10681.1"
                     /translation="TVNLRKGTELEDSESPLFFHYAIGLEGGLRIQMDRFHVLLGASG
                     EYGFMLGGTYNEEEENDTTGEEGKIKTTSFINNDLLIKYRGVIQYYF"
     gene            2033..2434
                     /locus_tag="PK754_14100"
     CDS             2033..2434
                     /locus_tag="PK754_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10682.1"
                     /translation="MIVRTAYMLIWVSMMTLTACDIGEMFSDEGTDDFLESQLGGGSG
                     GEGSATFYTQINYSGYIYIYVDNNYVGMLTQYYNSGNSPSCGANSGGAVTITNVSSGS
                     HNLSASDGSYSWSGSFYVSETHALKSRYNKG"
     gene            2561..3277
                     /locus_tag="PK754_14105"
     CDS             2561..3277
                     /locus_tag="PK754_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10683.1"
                     /translation="MRVSKKTWFNSLLILSITLGMACGDDKTSDNNVSVSFAGDIPPL
                     LTKGLKKSASHVTIDTAKILIKSIKLGGSGPSDSSDFKTGPYVLFITEGASLKTLSAA
                     NVPEGEYDQVKFEIHLPSQNESVPDPDFREGSGGNERYSAVIIGHYDGSRFVFKSKST
                     VNVQSDISPSVIVADSVGTANLTLGVSTSIWFTDGEVNLDPTNTNASNVAKIEQSIRN
                     SFYGFRDNNKDGDADVQIER"
BASE COUNT          981 a          647 c          804 g          983 t
ORIGIN      
        1 atagggtagg ttgccaattc aatcgcattg atcagattat ttttagcgtc cgtgcgaatg
       61 gcggataacg ggcgctggga tcccgtgagt atcacgggtt tggcaagatt ggacagcata
      121 taggacaaag cgctggccgt ataactcatg gtatccgtgc cgtgaataat gacgaacccg
      181 tcgtacgcgt ccatgttgtc ccaaataatg cgaccgagcg tcacccaatg ttccggcgaa
      241 agattggaac tgtccatgct aaaggagctg aaaaaatcca catcagcgat gtcccgtacg
      301 ccggggatat gccgaaatac ttcatccgca aattgttcgt gcggcaacgg agcgggatat
      361 ccgaatttca tccccatcgt tccgccggta tgtattaata atatcttttt cataaaagcc
      421 cgcgaatcta agtgaaatcg gagactttgg caaacgaatt tgagcatgtg atccaaaaaa
      481 agcggttagc ccaagctgcc gggttcggcg gaaaaaggta tataaaaaaa tattaagttt
      541 ttccttgcat ttacttgcca ttttgtatag tatcgctgcg cttaaagaag aaattgtttt
      601 taatatgatt gagccataaa ggagcgagtc atgaaacgtt gtgatgtgtg cggcaaaagc
      661 ccgatgtacg gcaacaatgt gagccatgcc aataaccgta ccaaacgccg ttggcttccg
      721 aatcttcagg aagtacgcgc taaaatgaat aacggtgctg ttaaacgcgt taaagtttgc
      781 acccaatgca tcaaatcagg aaaagtagct aaagcagcgt aattttctcg atttcgggtt
      841 tatcccaaaa aatgtaaaag cgtcgtcccc atcacggcgc ttttttattt ttattaaaaa
      901 aaaacgtttc atcgtttagt ttcgtcacgt tcattatcaa gttttcacag taatcctttc
      961 atttaagatt tacttcattg tgccgtcata cccggttcgg tttgccggtg cgaaaaatcg
     1021 tctgtcaaaa acataaatta tcaattcaat cgaagggagt gcctgatgta ccgacggctc
     1081 tttttgttgg tgtggtgtgc gccgattttg gctttcgcac aagtttttgt accggcaacg
     1141 cgtatgccca aaacatggat caatacgcta tcgctatcaa gcgggtttga atataatacg
     1201 tggagcccat ataaagcgga cgaacttcgt tatacgattg aaggctttca aatcggatac
     1261 ttgcgcggcg agatagatca taatgtgccg atgctgccga atctgaattt ttcgtgggaa
     1321 accaattttg gcggaaaaaa acaaaatgag ttgatgcagg cccagaatag cacatccaag
     1381 ctggagcaag ggtatgagaa attgcacacg gcattggaat ttggaaaaaa gaaaatctac
     1441 aacgatgccg gccaagttat caatacaatg gctaattggg agttgaccta tactaaagaa
     1501 acattttaca tagctgtcag ccccaagtcg tcggatttga ggtatgcggc gtattactcc
     1561 gatgctgttt ataattttcc acagggtact aagtattcga tgtttaccaa atttcaggaa
     1621 attggtttga cggcgtacac cggcggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnacag tcaatctccg taagggaaca gagttggaag attcggagag cccgttgttt
     1801 ttccattatg cgatcggttt ggaaggcggg ctacgcatac agatggatcg ttttcacgtg
     1861 ttgttgggtg cgtcggggga atacgggttc atgcttggcg gtacgtacaa tgaagaagag
     1921 gaaaatgata ccaccggaga agaaggcaaa ataaaaacga caagttttat taataacgat
     1981 cttctgatca aataccgcgg cgtaatacaa tattatttct aaaggatcat gtatgatagt
     2041 acgcacagca tatatgttga tatgggttag catgatgacg ctaacagcgt gtgatatagg
     2101 tgagatgttt tcggatgaag gaacggatga ttttttagaa tcgcagcttg gtggcggttc
     2161 cggcggagag ggttcggcta cattttatac gcagatcaat tattcaggtt atatctatat
     2221 ttatgtggac aataattatg ttggtatgtt gacccaatat tataattcag gaaattcacc
     2281 gtcttgtggt gcgaattccg gtggcgcagt gacaatcacc aatgtcagtt cgggatcgca
     2341 caacttgtca gcgtcggatg gctcttactc ttggtcagga agtttttatg tttccgaaac
     2401 acatgctcta aaatcaagat acaataaagg ctgattaagt atttttacgt aatttctcca
     2461 tcgtgtgata ttcatcacac ttagtttttt ataaagtaat acttttttgt agttactcat
     2521 tattaccacc aatatgatat acgataaaaa ggagagtgtc atgcgcgtgt cgaagaaaac
     2581 atggtttaac tcattgttga ttctctcaat tactttaggt atggcttgtg gcgatgataa
     2641 gacttcagat aataatgtat ctgtaagttt tgcgggtgat ataccgccgc tactgactaa
     2701 aggattaaaa aaatccgctt cgcatgttac aatcgacacg gccaaaattc ttatcaaatc
     2761 catcaaatta ggcggttcgg ggcctagcga ttcgtctgat tttaaaaccg gtccttatgt
     2821 tctttttatt accgaaggcg cttcgcttaa aacactaagc gctgccaatg taccggaagg
     2881 agaatacgat caggtcaaat ttgaaattca cctgccttca caaaatgaat cggttccgga
     2941 tccggatttt cgcgaaggtt caggaggaaa cgagcggtat tccgcagtga ttatcggtca
     3001 ctacgatgga agccggtttg tttttaaatc aaaaagcaca gtcaatgtac agtccgatat
     3061 ttcaccttcc gtgattgttg ccgatagcgt gggcacagct aatttgacgt tgggagtgag
     3121 tacttcaatt tggtttaccg acggtgaagt caatcttgat cctacgaata ccaacgccag
     3181 caatgtggca aagatcgaac aaagtatccg taattcgttt tatggatttc gagataacaa
     3241 taaggacggt gatgccgacg tacagattga gaggtagaat ttgtctgctg tattgctagc
     3301 gcagcattcg tattattatg atgcattgta agtaagtatg ttgataggat cgggggttta
     3361 cctgtttacg tgcaggtgaa tgtatttcac gttattaaaa tgaaaaggag ttcgtatgaa
     3421 agcgtttcgt tggtacttgt tcgccacagt gtttgcagcg tttgttgttt cgtgtggtga
     3481 ctcttcatcg aaatcgtcac aaaaagaggg aacta
//
LOCUS       DAOQNQ010000318         3509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_12954_length_3509_cov_5.832658, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000318 DAOQNQ010000000
VERSION     DAOQNQ010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12954_length_3509_cov_5.832658"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="PK754_14110"
     CDS             complement(<1..929)
                     /locus_tag="PK754_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HNB10684.1"
                     /translation="MIGPFRGGRTVGAVGVRQQPNVFYIGVNNGGVWKTEDFGRTWFP
                     IFDDQPTGSIGDIVVAPSDPNILYVGSGEGLQRPDLSVGDGVYKSTDAGKTWKHTGLR
                     DGQQIGGLAIDPENPDRVFAAVLGHPYGANTERGVFRTINGGSSWEKVLFIDENTGAI
                     QVTLDPKDTKTVYATLWAARQGPWENGSWQGPASGLYKSTDGGTTWKKLTQGLPTTKE
                     GLGRIGFCIAPSLSSRLYATVDSPEKGGLYRSDDGGNSWRLMNPDNRLWGRGGDFAEV
                     KVDPRNPDIVYSANVAFWKSTDGGKNWTCVRGAP"
     gene            1193..1693
                     /locus_tag="PK754_14115"
     CDS             1193..1693
                     /locus_tag="PK754_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10685.1"
                     /translation="MSVFLILCGKPFIKLLQPLQQPDSNDGIVALHDDLFMSVRWLSG
                     PMLPEIVWKKFSATGAFRTAFDVALQHVKNRRSVALHADFELLTVLDSDDQAWLRNEW
                     LSKAAQFGLKHIGVVLPVASIGKTSLMKGLRAPDGNILEIGYFMHREQSRDWILRMSR
                     MHRSSQ"
     gene            complement(1697..3025)
                     /locus_tag="PK754_14120"
     CDS             complement(1697..3025)
                     /locus_tag="PK754_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10686.1"
                     /translation="MSAIPYAVGYTFTDSSYEARVGGEFSVSIDRHTLKGTYNTDAST
                     LEADPFVFSFYKRKVSLAEKRPFFSRDLDVYRSPISLLYTRGIADINYGANYTFRSNT
                     LKAGAFYVEEEPIPGDTTHNIRRFMAVRPNVVTKFVNFGGTFIHNEQTNGPSETIISG
                     DARVDLPSRWVFVPQLATNRDGYASKAYLYYEFNPAGGPYADFSYTRFGKKFDGMTVI
                     NLFGKNYDEISNSVGYNFIYNRSYFHQMNARVEFYKAESLDRDFMLQRNINGNFFYKV
                     NDWLSVNHYAEISNADEALSDTTKKNHNDYAQDHNVKILIGNNAIVLGYKEGSYYGQK
                     LRNPYASYESMFFSRVGVSATAFLVENEARIFSAKINYRIMPKLFLRSLYQWKSEKGK
                     SNKYDNFQLWNSMLQYEFFAGSNLYLVLNLQGEDLEYVGRYCKLAYEFNF"
     gene            complement(3085..>3509)
                     /locus_tag="PK754_14125"
     CDS             complement(3085..>3509)
                     /locus_tag="PK754_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10687.1"
                     /translation="SSDALFFAIRFWPKGRVISQSFVRDRSSDEENEFFILLDLENKR
                     QNGYFFAFNFLGNQRDMRMYNQRQMSQEWDWNWQNKSTIFEDATPDKPGYIETEVRIP
                     VDKLQNKSPNTIGIDVQLFAYKQDGTSYFYRSALKVNS"
BASE COUNT          960 a          799 c          747 g         1003 t
ORIGIN      
        1 ggtgctcctc gtacacacgt ccagtttttg cccccgtcgg tggatttcca aaacgctacg
       61 ttcgcagagt atacgatatc cggatttcgc ggatctactt tgacctcggc aaagtctcca
      121 ccgcgtcccc aaagacgatt atccgggttc atcaaacgcc atgaattacc gccatcatca
      181 gatcggtata atccgccttt ttccggggaa tcaacggttg cgtataagcg ggatgataaa
      241 ctcggtgcga tacaaaaacc gatacgcccg agtccttctt tggtggtagg cagtccttgc
      301 gtgagcttct tccaagttgt accgccatcg gtcgatttgt acagaccgct tgcaggccct
      361 tgccaggaac cgttttccca tggaccttgc cgggcagccc aaagtgtagc atagacggtt
      421 ttagtgtcct tggggtccag agttacctga atagcgccgg tattttcatc aataaataaa
      481 actttttccc atgacgagcc accgttgatc gttctaaaaa caccgcgttc cgtatttgcg
      541 ccgtacggat ggcctaggac ggcggcaaaa acgcgatcag gattctcggg atctatcgcc
      601 aacccgccga tttgttgacc atcacgcaat ccggtatgtt tccatgtttt accggcatcc
      661 gttgatttat aaacgccgtc accgaccgat aaatcgggac gttgcaaacc ttcgccgctg
      721 cccacatata aaatattagg atccgaaggc gccacgacga tatcgcctat ggaaccggtc
      781 ggttgatcgt caaatatcgg gaaccatgtt cggccaaaat cttccgtttt ccacacgccg
      841 ccattattaa caccgatgta aaacacattc ggttgttgac gcacgccgac ggcgccgaca
      901 gttcgtccgc cacgaaaggg tccgatcatc cgccatcgaa aggctgccgc aacatctttt
      961 tcaaaatttt gagctgttgt gtaatcagaa catattatga gaatactcag tgcggtcatc
     1021 aacgaatggc gtaggtgtcg catgaataag attcctgtga taaagggtta tggaaacggg
     1081 ttcgattttt aaaagaagtg aagttactga aaccaatata cacgccgatt ttcaaaacta
     1141 aaaggaatga tataaaaatt ttcgtatcac ccaaatcggg tattgcttta gtttgtcagt
     1201 ttttttgata ttgtgcggta aaccgtttat caaactttta caacctttgc aacaaccgga
     1261 ttcaaacgat gggatcgttg cccttcatga tgatttattc atgtcggtac gatggctttc
     1321 cggaccgatg ttaccggaga tagtctggaa aaaattttca gccaccggcg cgtttcgtac
     1381 ggcatttgat gtggcgttac aacacgtcaa aaaccgtcgc agcgtcgcat tacatgctga
     1441 tttcgaactc ctgaccgtac ttgacagcga tgatcaagcc tggcttcgca acgagtggct
     1501 ttcaaaagcg gcgcaattcg ggcttaaaca tatcggcgtt gttttaccgg ttgcttccat
     1561 aggtaaaact tcgcttatga agggactccg tgctccggac ggtaacattt tagaaatcgg
     1621 ttattttatg catcgggaac aaagccggga ctggatcctg cgcatgtccc gcatgcatcg
     1681 ttcgtcccaa tagttattag aaattaaatt cgtaagcgag tttgcaatac ctgccgacat
     1741 actccaagtc ttcaccctga agattgagca ctaagtacaa attacttcct gcaaaaaatt
     1801 cgtactggag catgctgttc cataattgaa aattatcgta tttattggat ttgccttttt
     1861 cacttttcca ttgatacaat gatcttaaaa atagcttagg catgatacgg taatttattt
     1921 ttgcgctaaa tatacgcgct tcattttcga caagaaacgc cgtagcggat acgccaactc
     1981 tggaaaaaaa catggattcg tacgacgcat aagggttgcg taatttttgc ccatagtacg
     2041 aaccttcttt atagcctaaa acaatggcat tattccctat taaaatttta acgttatggt
     2101 cttgtgcata atcattatga ttttttttgg tcgtatcgct tagggcctca tcggcattac
     2161 ttatttcggc ataatgattg acgcttaacc aatcgttgac tttgtagaag aagttaccgt
     2221 tgatattgcg ctgcaacata aaatcacgat ctagtgattc ggctttgtaa aattcgacac
     2281 gtgcattcat ctgatgaaaa taggaacggt tgtatatgaa attataaccg accgaattgg
     2341 atatttcgtc gtaattttta ccgaaaagat ttattactgt cattccatca aatttttttc
     2401 caaatcgggt gtatgaaaaa tcagcatacg gaccgcccgc agggttaaat tcgtaataca
     2461 agtacgcttt ggaagcatat ccgtctctgt tagtcgccaa ctgtggcaca aaaacccaac
     2521 gggacggaag atccactcgc gcatcaccgc ttattatcgt ttccgacggt ccgttcgttt
     2581 gttcgttatg gataaaggtt ccgccaaaat tgacaaattt ggttaccaca ttaggccgaa
     2641 cggccataaa acgccttata ttatgcgtcg tgtccccggg gatcggctct tcttctacat
     2701 aaaatgcacc ggctttgagt gtattactgc gaaatgtata attggcacca tagttgatat
     2761 cggctatacc gcgggtataa agcaaactta tcggtgatcg gtacacatcc agatcacgac
     2821 tgaaaaatgg gcgtttttcg gctagagaaa ctttacgttt gtaaaaggaa aaaacgaaag
     2881 gatccgcttc taatgtcgat gcgtctgtat tatatgtacc tttaagcgta tggcgatcaa
     2941 tacttacact aaattcgccg ccaacacgtg cttcgtatga agaatccgta aacgtgtatc
     3001 ctacggcata aggtatagcg ctcatgttta tgttagcccg ttccacaaaa ggcgatatat
     3061 gcatttgata aggccctttg agtgttagga gttcactttc agggctgatc gataaaaata
     3121 actggtacca tcttgtttat aggcaaaaag ctgcacatca ataccgatgg tgtttgggct
     3181 tttattttgc agtttatcca caggtatccg tacttccgtc tcaatgtatc caggcttatc
     3241 cggcgtggcg tcttcaaaaa tcgtagattt gttttgccaa ttccaatccc attcttgcga
     3301 catctggcgt tgattataca tgcgcatatc acgctgatta ccgagaaaat tgaaagcaaa
     3361 aaaatatccg ttttgccgtt tgttttccaa atctaaaagg ataaaaaatt cattttcttc
     3421 atccgaactg cgatcgcgga caaacgattg agaaataacg cgccctttgg gccaaaaacg
     3481 aatggcaaaa aacaaggcat cggatgact
//
LOCUS       DAOQNQ010000319         3503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_13001_length_3503_cov_5.976508, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000319 DAOQNQ010000000
VERSION     DAOQNQ010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13001_length_3503_cov_5.976508"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1964
                     /locus_tag="PK754_14130"
     CDS             <1..1964
                     /locus_tag="PK754_14130"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNB10688.1"
                     /translation="KFCETIVGRSHEITDSLIVAMGDNGKKLTVTLGGNYVSSLANAN
                     AVKQGLISSSFIVILLILVLFFFFFGNFRSLAFIMIPILAGVTWVFCFADLMFEKVNI
                     ITAAAGAMLLGLGVDYAIHIYNRFVEQETHERHNTVEKNLDITFRETGTSVFYGAVST
                     AFVFIVLMVTEFRGLYELGFLGGVGILILFVAVVIVMPAEIKLRKRKNPRVNYLQGAF
                     SKLLGLNSRFVLKHPKYIATGALIITIFMIGVLTGAVPSKDEGLGVTFDDNIENIRSK
                     KDVDLQMIRRLEQKFGSHFKPISVVVSATSDDALIARLHKLNAKLDELTAKGMIKEYN
                     SMLRYIPSIEQQKHNLETIRNLDVESILFKIRLEMNKNGLRMNYFRLDRLRDMLQARE
                     PITIRNFEGEGFNEIMKHFYVERNGVKKVVTQVEFAGQSYEIDIVNQFVDEFNRDEQI
                     RNENVIITGIRVVTAEFLTLVKKDFQVAVIASLSAILLLVIVHYRKFRAVLVCMVPLT
                     FALLTIMGVMRLMGIKINFVNMITLPLLIGSGVDYGIYIISRYLEDQSHDVYAAIHET
                     GQSLFLSAATTVIGFGSLIFVDNRGLASLGYMCSFGIIICSVSSVIILPALLRLWGKK
                     IWKEAREEESPNKDTMIKKVTAAHTPEKK"
     gene            1961..2515
                     /locus_tag="PK754_14135"
     CDS             1961..2515
                     /locus_tag="PK754_14135"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNB10689.1"
                     /translation="MTRILIVMLAFVGCTPQPIYRAVKADGHGPSVKATETHKPTASV
                     SDSSATGAKKEPANTEEAMMAEIKTWIGTPYRFGMEKKSEGTDCSGFVGAVFRKVRQI
                     ELPRQAADMYAKGTPITRSELRFGDLVFFQNTYKGAVGASHVGIYVGNNQIAHAGTTV
                     GVTISSLEESYYEKHYLGCRRISQ"
     gene            complement(2601..>3503)
                     /locus_tag="PK754_14140"
     CDS             complement(2601..>3503)
                     /locus_tag="PK754_14140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB10690.1"
                     /translation="KKTANIADLLRESAQFSSAGSNVRCEFFIQDNLYPSDVDAGQIS
                     QVLNNLIINARQAMPNGGIIRILAQNHAIQSHNEGMPLKPGSYLKIAIEDNGCGISEE
                     HLQRIFDPYFTTKQTGNGLGLATSYSIIKNHDGAITVDSKINVGTTFTLYLPASKFAV
                     QQPRPQEPQALLSNGRILVMDDEESIREMASNMLNHLGFDAVSAKSGEEAVAMYEDAR
                     ARNKAFDAIIMDLTIPGGLGGKDAMARILSIDPAVCGIVSSGYSNDPVMADHTRYGFQ
                     GILPKPYFINDLSRVLNQLLKPKT"
BASE COUNT          908 a          739 c          888 g          968 t
ORIGIN      
        1 cgaagttttg tgaaacgatc gtaggacgca gtcacgagat taccgattcg cttatcgtag
       61 cgatgggtga taacggtaaa aaactcaccg tgactttggg cggtaactat gtttcgtccc
      121 ttgccaacgc caatgccgtc aaacaaggtc tgatcagcag ttcgtttatt gtgatattat
      181 taattcttgt gctcttcttt tttttctttg gtaatttccg ttcgctggcg tttatcatga
      241 taccgatact tgccggtgtg acgtgggtat tttgttttgc cgatctgatg tttgaaaaag
      301 tcaatatcat cacggcggca gccggtgcga tgttattggg gcttggggta gattatgcga
      361 tacatatcta taatcgcttt gtcgaacagg aaacgcatga gcggcacaat acggttgaaa
      421 aaaatctgga tatcacgttt cgcgaaaccg gcaccagtgt tttttacggc gctgtcagta
      481 cggcatttgt ttttatcgta cttatggtta cggagttccg cggattgtat gaactggggt
      541 ttctcggcgg tgtaggtatt ctgatacttt ttgtcgcggt tgtgattgtc atgccggcag
      601 agatcaagct tcgtaaacgt aaaaacccgc gtgtgaatta ccttcagggc gcattcagta
      661 aattgcttgg attaaattca aggtttgttc tcaaacatcc gaaatatatc gctacgggcg
      721 cattgatcat aaccatattt atgatcggcg ttttgacagg ggccgtaccc agcaaagatg
      781 aaggccttgg cgtcacattt gacgacaaca tcgaaaatat ccgcagtaaa aaagatgttg
      841 atcttcagat gatccggcgc ttggaacaaa aattcggctc gcactttaag ccgatcagcg
      901 ttgtcgtttc ggcgacttcg gatgatgctc tgatagcccg cttgcataaa ctcaacgcca
      961 aattggacga attgacggcc aaaggcatga tcaaagaata taattccatg ctgcgttata
     1021 ttccttccat cgagcagcag aaacacaacc ttgagaccat tcgtaatctg gacgtcgaga
     1081 gcattctctt taaaatccgg ttggagatga ataaaaacgg gcttcggatg aattattttc
     1141 gtcttgaccg tttgcgcgac atgttgcagg cgcgtgaacc catcacgata cgcaacttcg
     1201 aaggcgaagg ctttaatgaa atcatgaaac atttttacgt ggaacgtaac ggcgtaaaaa
     1261 aagtggtcac ccaagtcgaa tttgcaggtc agtcgtatga aatcgatatc gtcaatcaat
     1321 ttgtggatga gtttaatcgc gatgaacaga ttcgtaatga aaacgttatc atcacgggta
     1381 tccgcgtcgt gacggctgaa tttctcaccc tcgtgaaaaa ggattttcaa gttgccgtga
     1441 tagcttcatt gtcggcgata ttgctattag tgattgtgca ttatcgcaaa tttcgcgcgg
     1501 tactcgtatg catggtgccc cttacgtttg cattgctcac gatcatgggc gtgatgcggc
     1561 tcatgggcat caagatcaat tttgtcaata tgatcacgct cccgcttttg atcgggagcg
     1621 gcgtggacta tggtatttat attatttcgc ggtatcttga agatcaaagc cacgatgttt
     1681 atgcggccat tcacgaaacc ggccagtcac tttttctgag tgcggcaact acggtgatcg
     1741 gttttggttc attgattttc gtggacaatc gtgggttggc cagcttgggt tatatgtgct
     1801 cgtttggtat tattatctgt tccgtatcgt ccgtgattat cctaccggcg ttattgcgtc
     1861 tgtggggtaa aaaaatatgg aaggaagcac gtgaggaaga atcgccgaat aaggacacga
     1921 tgatcaaaaa agtaacagcg gcgcatacgc cggaaaaaaa atgacacgca ttttgattgt
     1981 tatgctggcg tttgtcggtt gtacgcctca accgatctat cgtgccgtga aagccgatgg
     2041 acatgggccg tcggtgaaag cgactgaaac gcataagccg actgcatcgg taagtgactc
     2101 atccgcgacc ggtgcaaaga aggaaccggc aaacaccgaa gaagcgatga tggcggagat
     2161 caaaacttgg ataggtacgc cttaccgttt tggtatggaa aaaaaatcag agggtacaga
     2221 ttgctcagga tttgtcggtg cggttttcag aaaagttcgt cagatcgaat tgccgcggca
     2281 agctgcggac atgtatgcca aaggaacacc aatcacacgc agtgaattac gatttggcga
     2341 cttggttttt ttccaaaata cctataaggg cgccgtcggc gcttcgcatg taggtatcta
     2401 tgtcggaaat aaccaaatcg ctcatgccgg tacgacggtc ggcgtaacga tatcatcact
     2461 ggaggagtcg tactatgaga agcattattt gggatgccgc agaatttcgc aatgatttcg
     2521 aaattgattt gaggtaacta cgctgatgcc gtttacaaaa tattcacggc atcataaact
     2581 ctctttagta gtctttaatt tcaggttttc ggtttgagaa gttgattgag cacgcgggat
     2641 agatcgttga tgaaataggg tttgggcagg atgccttgga acccgtagcg tgtatgatca
     2701 gccatgacag ggtcattgga atagccgctg gaaacaatgc cgcagacagc cggatcaatg
     2761 gacaaaatcc gcgccatcgc atctttaccg cctaaccctc ccggaatcgt gagatccata
     2821 ataatcgcgt caaaagcctt attacgtgcg cgtgcatctt catacatcgc gacggcttct
     2881 tcgcccgatt tggctgagac ggcatcaaat cccaagtggt ttagcatatt ggaggccatt
     2941 tcacgaatag actcttcgtc gtccatcacc aaaatccggc cattggataa aagcgcttgc
     3001 ggctcctgcg ggcgcggctg ctgaacggca aacttggatg ccggaagata cagcgtgaac
     3061 gtcgtaccca cattgatttt ggagtcaacc gtgatcgcac cgtcgtgatt tttgatgatc
     3121 gagtacgacg tcgcaagacc caagccgttg cctgtttgtt tagtagtaaa ataaggatca
     3181 aaaatacgct gcagatgctc ttcggaaatt ccgcaaccgt tgtcttcaat agctattttg
     3241 aggtaagagc cgggtttgag tggcatgcct tcattgtggg attggatggc atggttttgt
     3301 gcaagaatac ggataatgcc gccattgggc atggcttgac gcgcattgat gatcaagtta
     3361 ttgagcacct ggctgatctg accggcatcc acatcggaag gatacagatt atcttgaata
     3421 aaaaattcgc atcgaacatt ggaaccggct gacgaaaatt gtgcggattc ccgtaacaga
     3481 tcggcgatat tggcggtttt ttt
//
LOCUS       DAOQNQ010000320         3486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_13114_length_3486_cov_6.040804, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000320 DAOQNQ010000000
VERSION     DAOQNQ010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13114_length_3486_cov_6.040804"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PK754_14145"
     CDS             complement(<1..198)
                     /locus_tag="PK754_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10691.1"
                     /translation="MEYGLYKSMEWRQIGPFRGGRSVAVAGDVRDPLTFYFGATGGGV
                     WKTTDGGITWLNVSDGFFKTSA"
     gene            complement(414..1229)
                     /gene="speB"
                     /locus_tag="PK754_14150"
     CDS             complement(414..1229)
                     /gene="speB"
                     /locus_tag="PK754_14150"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNB10692.1"
                     /translation="MNAFPVFPSAGRIGLLGVAFDQNSSYQRGAAGAPQRIRKAFFCD
                     SSNLWSENEMDLGAEGIFTDMGDISPEPSQLLAETERAAGQLYESGLKVIAFGGDHSV
                     TFPLVKAAAKTHRNLSILHFDAHPDLYDDFEGNPHSHASPFARIMESGLAKRLVQVGI
                     RTINGHQRAQAQRFGVEVLTMQRWRDIFDVRWDSPLYISFDMDCLDPAFAPGISHREP
                     GGLSTREVLSIIQSLNADIIGADIVEYNPEMDINSTTDMVAAKVFKEIAAIML"
     gene            1376..2176
                     /gene="egtC"
                     /locus_tag="PK754_14155"
     CDS             1376..2176
                     /gene="egtC"
                     /locus_tag="PK754_14155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtC"
                     /protein_id="HNB10693.1"
                     /translation="MCRLVLYKGPAISLGHLLHTPKHSIIKQSYEAREMLRGTTNEDG
                     FGLAWYAPHWSDEPAVYRSIQPISNDVNMHTMAGKISSDIILAHVRGASEGMPVAITN
                     THPFGWQRYAFMHNGAVDEFRSRFFPELFNDIHPSLWSHIKGNTDSEHVFGLWLSYLG
                     LDKNASLHEQVSALRRTFQRLETIAQKQNLDIVLNIGISDGQNIIAARFHYGSRKATL
                     YYNDNLALFPDAVMIASEAQTDDAGWKPVPERSILTITSNNTLRVETV"
     gene            complement(2244..2792)
                     /locus_tag="PK754_14160"
     CDS             complement(2244..2792)
                     /locus_tag="PK754_14160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HNB10694.1"
                     /translation="MHIVLFLGSLRKDSFNRKLLNNVLPMLEGHTTDVVDLKETVLPV
                     FDGDLDGDKTPPETQRLAERVKRADAVIFVSPEYNYNIPGGLKNAVDWVSRSKERPLH
                     DKPVMLTGATQGPFGCVRLFIAMRQMLVFFNAFVIPRQVGIPSAAKAFDEQGKLIDPK
                     QVEMLQTAVHELLHVTQALSKK"
     gene            complement(2916..>3486)
                     /locus_tag="PK754_14165"
     CDS             complement(2916..>3486)
                     /locus_tag="PK754_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNB10695.1"
                     /translation="VRARVPKLKLDDLFEKKDEIADAVRHELSDTMEDFGYAVLKALV
                     TDIDPDAKVKEAMNEINAATRMRMAAAEKGEADRILKVKAAEAEAQSKALQGKGIADQ
                     RKAIIEGLRDSVEQFQQAVEGSSAKDVMMLVLMTQYFDTLQNLGEHSHTNTILMPHSP
                     GGLNAIADQIRDAMITAGEANKKQPAKPQ"
BASE COUNT          913 a          899 c          760 g          914 t
ORIGIN      
        1 cgcgctggtt ttaaaaaatc cgtccgatac attgagccac gtaataccgc cgtctgtcgt
       61 tttccataca ccgccgccgg tcgcaccaaa ataaaaggtc aacggatcgc gcacgtcacc
      121 ggcaaccgcg acggaacggc ctccgcgaaa cggaccgatt tggcgccact ccatagattt
      181 gtacaacccg tattccatgc cgctcggcac ttccgttttt ttctgtgtaa aaagaaaccc
      241 cggcatcagg atcattagca taccgacaaa acacacatgt ttccgattca tagattcctc
      301 actacgcgtt gggttattac ttactcatat cgaatgattg atttgacgtt tccgcgttta
      361 accaaacagt catcaggttt tcttgtcttt acaaaataaa aaagcaagaa aacctacagc
      421 atgatcgcag ctatttcctt aaatactttt gccgctacca tatccgtcgt gctattgata
      481 tccatttccg ggttgtattc gacaatgtcg gcgccgatga tatcggcatt aagtgattgg
      541 atgatactta acacttcccg cgtggaaagc cctccgggtt cgcgatgtga tatacccgga
      601 gcaaaagcag gatcaaggca atccatatca aaactaatat aaagaggaga atcccatcgt
      661 acatcaaaaa tatcacgcca gcgttgcatc gtaagaacct ccacaccaaa acgctgtgct
      721 tgcgcacgtt gatgtccatt gatggtgcga attccaacct gcaccagacg tttcgccaaa
      781 cccgactcca tgattcgtgc aaagggactg gcgtgcgaat gcgggttgcc ctcgaagtca
      841 tcatacaaat caggatgtgc atcaaagtga agaatactga gattacgatg cgttttcgct
      901 gccgctttga ccagcggaaa agtcacggaa tgatcgccgc caaaggcaat gaccttcaaa
      961 ccggattcat aaagttgccc ggctgcacgt tccgtctccg caagcaattg cgacggctcc
     1021 ggcgatatat ctcccatatc cgtaaaaatg ccttcggcac caagatccat ttcattttca
     1081 ctccaaagat tggaggaatc gcaaaagaat gctttgcgga tccgctgcgg agcgcccgct
     1141 gcgccacgtt gataggatga gttttgatca aatgctacgc ctaataaacc gatgcgtccg
     1201 gctgacggaa atacgggaaa agcgttcata ttggcctcgt gacctcacag gaatgtttta
     1261 taattttttt agtgtacgga gcacccatac gcacgcgtta agctaaacac gtacaatgat
     1321 gtgtgtttta tttttcatcg ttggtttttt tccattcaaa gcgtatcata cacccatgtg
     1381 ccgattagtt ttatacaaag gcccggcaat atcgctaggt cacctgcttc acacaccgaa
     1441 gcattcgatc atcaaacaaa gttatgaagc gcgggagatg ctgcgcggaa caaccaacga
     1501 agacggattc ggactggcgt ggtatgcgcc gcattggtcc gacgaacccg ccgtttatcg
     1561 cagtatccaa ccgatcagca atgatgtcaa catgcatacg atggccggca aaataagttc
     1621 ggacatcatt ttggcgcatg tgcgcggcgc ttccgaaggt atgcccgtcg cgatcaccaa
     1681 tacacatcct tttggttggc aacgttatgc ttttatgcac aacggcgccg tggatgaatt
     1741 tcgctcacgg ttttttccgg aattattcaa cgatattcat ccgtcgcttt ggtcgcacat
     1801 caaaggcaat accgattccg aacatgtttt cggattatgg ttaagttacc ttggtttaga
     1861 taagaatgcg tcgttgcatg aacaagtatc ggcgcttcgt cgcacatttc agcgattgga
     1921 aaccatcgca caaaaacaaa acttagacat cgtattgaat atcggaattt ccgacgggca
     1981 gaacatcatt gccgcacggt ttcattacgg aagccgcaag gctacattgt attacaacga
     2041 caacctcgcg ttatttcccg acgctgtgat gatcgcatcc gaggcgcaaa ccgacgatgc
     2101 aggatggaaa cccgtacccg aacgcagtat cctcacaatt acttcaaaca acacccttcg
     2161 cgtcgaaaca gtttaaaaac ttatctccaa ttcaaacctg taataaacgc acgttgatta
     2221 agcgcgatat ttttcgaagc cctctatttt ttgcttaatg cctgtgtcac atgtaataat
     2281 tcatgaaccg cggtttgcag catttcgacc tgtttaggat cgatgagttt cccttgttca
     2341 tcaaacgctt tggcggccga cggaatacct acctgacgcg gaataacgaa cgcgttaaaa
     2401 aatacgagca tctgccgcat agcaataaat aaacgtacgc acccgaacgg cccttgtgtt
     2461 gcaccggtga gcatgacggg tttatcatgc aacggacgct ctttgcttcg cgaaacccaa
     2521 tccacagcgt ttttcaaacc gcccggtatg ttatagttat attccggtga aacaaaaata
     2581 accgcatcgg cacgttttac acgttcggca aggcgttgcg tttccggagg cgttttatca
     2641 ccgtccagat caccgtcaaa taccggaagc acggtttctt tcaaatctac cacatccgtt
     2701 gtgtgccctt caagcatcgg caaaacatta ttcaatagtt ttcggttgaa cgaatctttg
     2761 cggagactgc cgagaaaaag tacaatatgc ataacgatgc gctccttgtc tttatgtttt
     2821 gttaacgaaa ttctccgagt gattcgctca ggatataaaa acgggtccgt tacagaaata
     2881 ttccgcacac tgacccgtcc gatttaacaa agaaattatt gcggtttggc cggctgtttt
     2941 ttatttgcct ctccggccgt gatcatcgca tcgcgaatct gatcagctat cgcattgagt
     3001 ccgcccgggg aatggggcat cagaatcgta ttggtatgtg aatgttcacc cagattttgc
     3061 aacgtatcaa aatactgcgt catcaaaaca agcatcatta cgtctttggc ggaagaacct
     3121 tcgaccgcct gctgaaattg ttcgacggaa tcgcgcaatc cttcgatgat ggctttacgt
     3181 tgatcggcga tacctttccc ctgaagtgct ttactctgcg cttcggcctc ggccgctttg
     3241 accttgagaa tacgatcggc ttcgcctttt tcggccgcag ccatacgcat acgcgttgcc
     3301 gcattgattt cattcatcgc ttctttcact ttggcatccg gatcgatgtc ggtgaccagc
     3361 gctttgagta cagcgtatcc gaaatcttcc atcgtatcgc tgagttcatg acgcacggca
     3421 tcggcgatct cgtctttttt ctcaaacaaa tcatctaatt tgagcttggg cacgcgcgca
     3481 cggaca
//
LOCUS       DAOQNQ010000321         3482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_13139_length_3482_cov_3.812081, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000321 DAOQNQ010000000
VERSION     DAOQNQ010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13139_length_3482_cov_3.812081"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="PK754_14170"
     CDS             <1..922
                     /locus_tag="PK754_14170"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB10696.1"
                     /translation="GELAAAFNQFLNKLEEIMRQVARTSEQISVSVESISKASSDVAG
                     GAEQQSNQATLVAVAAEEMSATIVQTTANTGDAVRLSQQAEEATDRGKMVVEDTVNGL
                     QNISSVVSESAQTLLELGKTVAQIGTVIEVINDIADQINLLSLNASIEAATAGDAGKG
                     FAVVANEVKNLAEDTTRSTTEISQMIEKIQHAMANAIRAMERGTMEVEKGRALGHKTA
                     EAFDEILKASEHVREMITNISVSSQQQSHSAEEISRNIETIAGITRNTAQGVRQIDGT
                     TDALVNHTSALKTLVGRFKLNEQDRAQNYN"
     gene            891..1700
                     /locus_tag="PK754_14175"
     CDS             891..1700
                     /locus_tag="PK754_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HNB10697.1"
                     /translation="MSKIVHKITTNSPRKTIYTDLMQVCEQRGSGYFVLIDPDKKTEA
                     ETLQTVSHCAEAGVDAILIGSSLMLSSRFGSTIQAIKKEFSLPVIIFPGSTIQLSEYA
                     DALLFLSLISSRNPEFLITTQVLGAPIVRKMGLEAISTGYMLIESGKVTSAEFMSNSK
                     PIPRDKNDIAVAHALAGEYLGMKTIYLEAGSGALHSVPAEMVSAVSEYVNIPVIVGGG
                     IRTPEDALQKVEAGASFVVTGNVLEHSGSLSLIREFANAIHWKTPAAQKHA"
     gene            1706..2422
                     /locus_tag="PK754_14180"
     CDS             1706..2422
                     /locus_tag="PK754_14180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNB10698.1"
                     /translation="MGIRRRALLVFAKLPEPGKVKTRLIPALGKDGACSLYQAFLKDS
                     IIQYTRLALEVGYTPYLFVTPSEAVSFFENSCDTLTSEIGTNNAWQVRLQSQGDLGAR
                     MKDAFAGLFAQNHDACWIIGTDHPTLPDAYVVDAFRYLDQYDAVIGPADDGGYYGLGL
                     QRRMDFLFEDIPWSTEQVLSTTKLRMQNNRVNYYPIKPWYDVDEPADMYRLKRDLDKA
                     GMRCNYTKQWFEQHGMTYGR"
     gene            2412..2885
                     /locus_tag="PK754_14185"
     CDS             2412..2885
                     /locus_tag="PK754_14185"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNB10699.1"
                     /translation="MADKQKNLKFLHFRSGNSDYAVELEYVKEIIRSVAVSPMNELPP
                     FAPGVINLRGEIIPIIDFLLRSGAGATTLRLKSRIIITRIHDITAGLLVDDVLETLEV
                     EPSDITRNLHADVLIDVKYIQGTFLSGGTTIICVDLNKLMTDKEYAVLRHEVKHA"
     gene            2878..>3482
                     /locus_tag="PK754_14190"
     CDS             2878..>3482
                     /locus_tag="PK754_14190"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNB10700.1"
                     /translation="MLNYHEVHPMGTGHGVIAKEADFTDQDFIRFRNLIRARSGIYFN
                     DRKRADLKSGVLKAFHYSGVSGLDKYFDLLASDPTQSAQFKTLVSFLTVGETYFFRHF
                     DVIEKVVLPSLIKAHHHDKTIRIWSAGCSTGEEPYTVAMVLHNLLPDIRDWKISIVGT
                     DINVNSVEYAKEGVYRPWSLRSITDFYKNFYFDKKDGLYYI"
BASE COUNT          919 a          754 c          906 g          903 t
ORIGIN      
        1 tggtgagtta gctgcggcgt ttaatcaatt tttgaataag ttagaagaaa tcatgcgtca
       61 ggttgcgcgg acatcggaac agatttcggt atcggtggag tcgatcagca aggccagttc
      121 cgacgtcgcc ggcggtgcag aacaacaatc caatcaggct acattggtgg cggttgcggc
      181 cgaagagatg tccgctacga tcgtacagac aacagccaat accggtgatg cggtgcggct
      241 atcccagcag gctgaagaag ctaccgatcg gggcaaaatg gttgttgagg atacggtcaa
      301 cggattgcaa aatatttctt cggtggtcag tgagtcggct cagacgttgc tggagttggg
      361 taaaactgtc gctcagatcg gtacggtgat cgaggttatc aacgacattg ccgaccaaat
      421 caacttattg tcgctcaatg cttcgatcga agccgcgaca gccggtgatg cgggaaaagg
      481 ttttgccgtt gtcgccaatg aagtgaaaaa cttagcggag gatacgacac gttccacgac
      541 ggaaatatct cagatgatcg aaaaaatcca gcatgctatg gctaacgcga tacgggcgat
      601 ggagcgcgga acgatggaag tagaaaaggg acgtgctttg gggcataaaa cggccgaggc
      661 ctttgacgaa attttgaaag ccagtgaaca tgtccgcgaa atgataacga atatttccgt
      721 ttcatcccag cagcagagcc attcggccga agaaatttcc agaaacattg aaaccattgc
      781 cggtattaca cgcaatacag cccagggcgt gcgccaaata gacggtacca cggatgcttt
      841 ggtcaatcat acgtctgcac taaagaccct tgtcggacga tttaaactga atgagcaaga
      901 tcgtgcacaa aattacaact aactctccgc ggaaaacgat ttatacagac ctcatgcagg
      961 tttgcgaaca gcgcggttcg ggctattttg tactcattga tcccgacaaa aaaacggaag
     1021 cggagacgct gcagaccgtg tcccattgcg ccgaagcagg tgtggacgcg attttgatcg
     1081 gaagcagcct tatgctcagc agccgatttg gcagtacgat ccaggccata aaaaaagaat
     1141 tttctttacc cgttattatt tttccgggta gcacgattca gctttccgaa tacgcggatg
     1201 cgttattatt tttgtccctt atcagcagcc gcaatccgga gtttttgata accactcagg
     1261 tgcttggggc gccgattgtt cgtaagatgg gattggaagc catttcgacg gggtatatgc
     1321 ttatcgaatc cggtaaagta acgtccgctg aattcatgag taattccaaa cccataccgc
     1381 gcgataaaaa tgacattgct gttgctcatg ctttggcggg tgaatacctc ggcatgaaaa
     1441 caatttatct cgaagccgga agcggagcgt tgcattctgt gccggcagaa atggtttctg
     1501 cggtcagtga gtatgtgaat ataccggtga tcgttggcgg gggtattcgt acacccgaag
     1561 acgcccttca aaaagtggag gccggcgctt cgtttgttgt gaccggtaat gtgctggaac
     1621 actccggaag cctgtcattg attcgtgaat ttgcgaatgc aatacactgg aaaactccgg
     1681 cggctcaaaa acacgcgtga gtaatgtggg catacgacgg cgtgcattgc tggtgtttgc
     1741 caaattaccc gagccgggca aagtaaaaac tcgattgata ccggcccttg gaaaagacgg
     1801 ggcctgcagt ttatatcagg cttttttgaa agacagcatt attcaatata ccagacttgc
     1861 tttggaagta ggttatacac cgtatttatt tgttacgccg tcggaagcgg tttcgttttt
     1921 tgaaaattct tgtgatacgc taacttcgga aattgggacg aacaacgcat ggcaagtgcg
     1981 attgcaatca caaggggatt tgggcgcccg tatgaaagac gcgttcgccg gtctttttgc
     2041 tcaaaaccat gatgcatgct ggattatcgg gaccgatcac ccgacgttgc cggatgctta
     2101 tgtggtagat gcttttcgtt atttggatca atatgatgcc gtcatcggcc ctgccgacga
     2161 tggcggttac tatggtttgg gcttgcagcg aagaatggat tttttatttg aagatatacc
     2221 ttggagcacg gaacaagttt tgtccacaac taagctacgg atgcagaaca atagagtgaa
     2281 ttattacccg atcaagccgt ggtatgatgt ggatgagccg gcggatatgt atcgcctcaa
     2341 acgtgattta gacaaggccg gtatgcgatg taattatacg aagcaatggt ttgaacaaca
     2401 tgggatgaca tatggccgat aaacaaaaaa atctgaaatt ccttcacttt cgatccggca
     2461 acagcgacta tgccgttgaa ctggaatacg tgaaagaaat tatacgcagc gttgccgttt
     2521 ctccgatgaa cgagctgccc ccgtttgcgc cgggcgtcat caatctccgg ggagaaatca
     2581 taccgattat tgatttcctg ttgcgttcgg gcgccggtgc gacgacgttg cgtctgaaat
     2641 cgcgcatcat cattacgcgg attcatgata ttacggccgg tctgcttgtt gatgatgttt
     2701 tggaaaccct tgaagtcgaa ccatcggata ttacacgcaa tttgcacgcc gatgtcctca
     2761 ttgacgtcaa atatattcag ggcacatttt tgtccggcgg tacgacgatc atttgtgtag
     2821 atttgaataa attgatgacg gacaaggaat acgctgtttt acgtcacgaa gtcaaacatg
     2881 cttaattacc acgaagtaca tccgatgggc accgggcatg gcgttatcgc caaagaagcc
     2941 gattttacgg atcaggattt tatccggttt cgtaatctga ttcgcgcccg cagcggtatt
     3001 tatttcaacg accgcaaacg tgccgatctc aaaagcggag tattgaaagc gtttcattat
     3061 tccggcgttt ctggtctgga taagtatttt gacttgttgg cgtcggatcc gacacagtcc
     3121 gcgcaattta agacgcttgt ttctttcctt accgtcggag aaacgtattt tttcagacat
     3181 tttgatgtga tcgaaaaagt cgttttaccg agccttatca aagcacatca tcacgataaa
     3241 accattcgca tatggagcgc cggttgttct accggggaag agccgtatac cgttgcgatg
     3301 gtattgcata atctgttacc ggatatccgg gattggaaga tttccatcgt gggtacggac
     3361 atcaatgtga attcggtgga gtatgccaaa gagggagttt acaggccgtg gtcgctgcgt
     3421 tcgatcaccg atttttataa aaatttttat ttcgataaaa aagacggatt gtactacatc
     3481 aa
//
LOCUS       DAOQNQ010000322         3462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_13264_length_3462_cov_5.603757, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000322 DAOQNQ010000000
VERSION     DAOQNQ010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13264_length_3462_cov_5.603757"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PK754_14195"
     CDS             complement(<1..262)
                     /locus_tag="PK754_14195"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005954597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNB10701.1"
                     /translation="MKSDIEIAQESPITPITEIAKKIGLNASDIELYGEHKAKIKLEV
                     LDRLKDKKIGKFIFVTAINPTPLGEGKTVVNIGLSQALAKIGK"
     gene            complement(398..1168)
                     /locus_tag="PK754_14200"
     CDS             complement(398..1168)
                     /locus_tag="PK754_14200"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB10702.1"
                     /translation="MKLHFVEYGSPKTLVILHGLLGSERNWYSVAKKLSDHYRLIIPD
                     LRNHGVSPHDPEHSIVAMRKDIEALVDRLHLHEFYLLGHSMGGHVAMNYAFAHPERVK
                     ALIVEDIAPRSYGTGLIEILTAMNAVDLNLYKEKKQVEVALAEGIKNPAVRSFVITNL
                     VRDHDRMYWRVNLPALTEFAANEIVRFKASENDRFHGPTLFVGGEKSMYNLRDDAELI
                     AKHFPSSALEMIPDAGHWIHHEKADVFCQIVMDFLNRH"
     gene            1310..2158
                     /locus_tag="PK754_14205"
     CDS             1310..2158
                     /locus_tag="PK754_14205"
                     /inference="COORDINATES: protein motif:HMM:NF015927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HNB10703.1"
                     /translation="MDITYLTFVLGAVVTLLILARLVNQFLMRERLTDALTEKDRLAT
                     GIALSGYLFGVMMIIIDVLSGEGHNDWLKDFLLVGIYGLAGIVFLVFVSVMELRLILS
                     AKALDAVREGNVAAAITVAASYIATSQIIGAVVSGDNTGGNWLTAVIFFIIGQATLLI
                     MTYVFRWLTAYNDAEAIMQGNAAAALSYAGVMIGIAIIVGTALRGDFIDYPTSLIDYA
                     WSLVIVVALYPIRQFLVQGVLLGCGFSFYGGKLDEEISKDRNIGAGCIEATTYIASAL
                     LATALL"
     gene            2172..>3462
                     /locus_tag="PK754_14210"
     CDS             2172..>3462
                     /locus_tag="PK754_14210"
                     /inference="COORDINATES: protein motif:HMM:NF015683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HNB10704.1"
                     /translation="MRTSEVKYTDPSVPRRDPYFDFAKKITDSHILTDPWIDGDPRFS
                     DEPVILGAGTYALMQKAAEHIGALYDELVRIILREPHWLDDYFHLTPHQKMLWFASGG
                     RWHGMARLDMFLCTDGRLQVCEMNSDTPSGEAEAVLINQIIRSRFLRFTDPNAGLETQ
                     FIRMVLRLWGKRTWPRLSPTVGIVYPTEMPEDLSMIRLYQRWLESRGAHIILGSPYNL
                     GSTAQRLTLLQKPVDVLIRHYKTDWWCERLPVWKDQEPFPDALPLVKPLQHLMRAWLA
                     GQITMINPPGAVLTQNKLTMAFFWEHLRKFSPTARNTIRTWIPETRRLITKESKKLNK
                     NEWVLKSDYGCEGDEVIIGNDVSETLWKESLSKAIPKHWILQRYFSSIRIGRENLIPN
                     FGLYLVGGRAAGIYTRLSNTATDGTALSAATLIDQHSH"
BASE COUNT          865 a          832 c          803 g          962 t
ORIGIN      
        1 tttttcctat tttggccaac gcttgcgaca atccgatatt gacgacggtc ttgccttcac
       61 cgagcggcgt tgggttgatc gccgtgacaa aaataaattt tccaattttt ttgtccttca
      121 gacggtccaa tacttctagt ttgatcttgg ctttatgttc accgtacaac tcaatatcgg
      181 aggcattcaa tccgatcttt ttggcaattt cagtaatcgg cgtaatgggt gattcctgag
      241 caatttcaat gtcgcttttc atatatcaac ctcataatag atgttttcga ttggtcaatt
      301 ctacgatttc gatatatacg attccatgac taaagtcatg tctaaaaaag gtaggttttc
      361 ccgatctaaa aagaatgctc tgatatcaat ggtacgttta gtggcgattc aaaaaatcca
      421 tcacgatttg acaaaataca tcggcttttt catgatgaat ccaatgcccg gcatccggta
      481 tcatctctaa tgctgacgaa ggaaaatgtt tagctattag ctccgcgtca tcacgaagat
      541 tatacatgga tttctcaccg ccgacaaata gcgtaggacc gtgaaaccta tcgttttccg
      601 aggctttgaa tcgtactatt tcattggctg caaattccgt caaagccgga agattaacac
      661 gccaatacat ccgatcgtga tcacgcacca ggttggtaat cacaaaggat cgtacggccg
      721 gatttttgat tccctctgca agggccactt ccacttgttt tttttcttta taaagattca
      781 gatccacggc attcatcgct gtgagaattt cgatcaaacc cgtgccgtat gaacgcggtg
      841 caatgtcttc tacgatcaac gctttaactc gctcgggatg tgcaaaagcg taattcatcg
      901 cgacatggcc acccatcgaa tggcccaaca agtaaaattc atgcaaatgc aaacggtcaa
      961 ccaacgcctc aatatcttta cgcatcgcca cgatagaatg ttcaggatcg tgcggcgaaa
     1021 ctccgtggtt gcgcagatcc ggaatgatca agcggtaatg gtcggataat tttttggcta
     1081 ccgaatacca gttgcgttca ctgcccagca ggccgtgcag tatcaccagt gttttcgggc
     1141 tgccgtattc gacaaaatgt aatttcatac gctccgttat attggcgcgg aagttagttt
     1201 cgcttatatc caattgcaac ggtttttaca aaacgaagga ttattaccga atcggcttgc
     1261 gcttggtttg gcgctgttct aatttaaaga aaaaacaaca gatcaatcta tggacattac
     1321 ttacctcaca ttcgttttag gcgccgtcgt tacacttttg atccttgcgc gcctcgtcaa
     1381 tcaatttctc atgcgggaac gcctcaccga tgcgctaact gaaaaagatc gtttggcaac
     1441 cggtatcgcg ctatcgggtt atttattcgg cgtgatgatg atcatcatag acgtgctcag
     1501 tggcgagggt cataacgatt ggcttaaaga ttttttactt gtcggaattt acggactggc
     1561 cgggatcgta tttttggttt ttgtcagtgt gatggagctg cgtctgatcc tctccgccaa
     1621 agcattggat gccgttcgtg aaggcaatgt cgccgccgcc attacggtcg ctgcttcata
     1681 cattgcgaca tcgcagatca tcggcgccgt cgtatccggc gataataccg gcggcaattg
     1741 gctgacggca gttatttttt tcattatcgg tcaggccacg ctgctgatca tgacgtatgt
     1801 atttcggtgg ctcacagcgt ataacgatgc cgaagccatc atgcaaggta atgctgccgc
     1861 cgcgctttca tatgccgggg tcatgatcgg tatcgctatt attgtaggca cagccttacg
     1921 cggtgacttc atagactacc ctaccagcct tatagattat gcatggtcgc tggtgatcgt
     1981 cgtggcactt tatccgatcc gccaattttt agtgcaaggt gttttactcg gatgcggatt
     2041 tagtttttat ggcggtaaat tagatgaaga aatttccaaa gatcgcaata tcggcgccgg
     2101 atgtattgaa gcaacgacct atatcgcctc ggcactttta gccacggcat tactgtagta
     2161 acattaaatc tatgcgcacc tccgaggtaa aatacaccga tccttcggtt ccgcgacgcg
     2221 acccgtattt cgattttgca aaaaaaatca cggattcgca tatcctcacc gatccttgga
     2281 ttgacggcga tccgcgtttt tcggacgaac cggtgatcct cggcgccgga acttatgcat
     2341 tgatgcaaaa agcggccgaa cacatcggcg ctttatatga tgaattggtg cgcatcattc
     2401 ttcgcgaacc gcattggctc gatgattatt tccatttgac gccgcatcaa aagatgcttt
     2461 ggtttgcctc cggcggacga tggcatggca tggcgcgatt ggatatgttt ttatgtacgg
     2521 acgggcgtct gcaagtatgt gaaatgaatt ccgatacgcc cagcggtgaa gcggaagccg
     2581 ttttgatcaa tcagattatt cgatcgcgct ttctgcgttt taccgatccc aatgccggat
     2641 tggaaacgca atttatacgt atggttttac gtctctgggg caaacgtaca tggcccaggc
     2701 tttctccgac ggtcggcatc gtgtatccga ccgaaatgcc cgaagatctc tccatgatac
     2761 gcttgtatca acgttggttg gaatcccgcg gcgctcatat catattgggt tcgccctaca
     2821 atctgggcag cactgcgcag cggctgacgt tactgcaaaa acccgtggat gtattgattc
     2881 gtcattacaa aacagattgg tggtgcgaac gcttgccggt gtggaaagat caggaacctt
     2941 ttcccgatgc gcttccgctc gtcaaaccat tgcaacacct gatgcgcgcc tggcttgcgg
     3001 gacagataac gatgatcaac ccgcccggcg ctgtgcttac acaaaacaaa ctaacaatgg
     3061 cttttttttg ggaacacctg cgtaagtttt cacctacggc gcggaatacg atacgcacgt
     3121 ggatcccgga aacgcggcgc ctgataacga aggaaagcaa aaaactgaat aaaaatgaat
     3181 gggtactcaa atccgattac ggatgcgaag gcgatgaagt gatcatcggt aacgatgtca
     3241 gtgaaacact gtggaaagaa tcgctgtcca aagctattcc caaacactgg atccttcagc
     3301 gttatttttc gtcaatccgc atcggccgcg aaaatctaat tccaaatttc ggactgtatc
     3361 tcgtcggagg gcgtgcggcc ggtatttata cacgtttatc caatacggcc acggacggta
     3421 cggcgctcag cgcagcgaca ttgattgacc aacattctca ct
//
LOCUS       DAOQNQ010000323         3459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_13288_length_3459_cov_6.465922, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000323 DAOQNQ010000000
VERSION     DAOQNQ010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13288_length_3459_cov_6.465922"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PK754_14215"
     CDS             complement(<1..314)
                     /locus_tag="PK754_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020023430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNB10705.1"
                     /translation="MSTIEKLVPRNIEDEMRECYIDYSMSVIVSRAIPDVRDGLKPVH
                     RRVLFGMNELGMSYNKPYKKSARIVGEVLGKYHPHGDTAVYDTIVRMAQPFSLRYMLV
                     DG"
     gene            complement(355..2289)
                     /gene="gyrB"
                     /locus_tag="PK754_14220"
     CDS             complement(355..2289)
                     /gene="gyrB"
                     /locus_tag="PK754_14220"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNB10706.1"
                     /translation="MAEKEQKKTGAKYDAENIQVLEGLEHVRRRPAMYIGDVSTRGLH
                     HLVYEIVDNSIDEAMAGYCTEINVSINEDNSITVVDNGRGIPVDMHPTEKKPAVEVVM
                     TVLGAGGKFDKNTYKVSGGLHGVGASVVNALSEWCEVEVRRNGKMYRQKYKAGRSTTK
                     VDEVGKSKESGTTTTFMADSTIFKKIVYTFDTLAARMRELAFLNRNLKITLEDKREKN
                     RREEYHYEGGISQFVQYLDESRAALMKKPIYIETEKENVPVEIAFQYNDSYNENVFSY
                     VNNINTVEGGTHLSGFRTALTRTLNNYATKNNLVKEKEGISIQGDDTREGLTAIISVK
                     IAEPQFEGQTKTKLGNSEVKGIVEQAVGEGLMEFLEKSPGVAKQIIDKCLQAARAREA
                     ARKARDLTRKKNMLESSGLPGKLADCSISDPQYCEIYLVEGDSAGGSAKQGRDRRFQA
                     ILPLKGKILNVEKARLDKMLSNEEILTIVSAIGTGIGTDDFNLANLRYGKVIIMTDAD
                     VDGAHIRTLLLTFFFRHMRQLVENGNLYIAQPPLYRLKKGKEEKYAFDDEEKAAMLKQ
                     WGAKEEDTNIQRYKGLGEMNPEQLWSTTMDPDKRVMMQVTIEDAATADHVFSTLMGDA
                     VEPRRKFIEDNAKYVRNLDV"
     gene            complement(2342..2731)
                     /locus_tag="PK754_14225"
     CDS             complement(2342..2731)
                     /locus_tag="PK754_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNB10707.1"
                     /translation="MHKDVKAYHGQLSDEDRLICETLYAEISRGLPEAENKVWHAHPV
                     WFLDGNPIVGYSKLKSCIRLLFWSGQSFDEPGLQNEGRFKAAEKRFTSADQVIKKDLA
                     RWLKKAKTIQWDYKNIVKRKGVLERLK"
     gene            complement(2749..3045)
                     /locus_tag="PK754_14230"
     CDS             complement(2749..3045)
                     /locus_tag="PK754_14230"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNB10708.1"
                     /translation="MKPKKTDATPLGEALDKLLKRYKIADRVHEQVVLEQFERLMGTA
                     FTQRAKAVKIEDGLLFLEVVNSVWRQELFYQKRNIIERINSALGEKIVREIVFR"
     gene            complement(3042..>3459)
                     /locus_tag="PK754_14235"
     CDS             complement(3042..>3459)
                     /locus_tag="PK754_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNB10709.1"
                     /translation="ETLPDDQSIAEAMAKTIAENRDREMMRQTSLIGPHKDELLLLLN
                     DKAIREFGSQGQLKTMALALKLAEFLHMAERRELQPLLLLDDVFSELDWTRRHNLIRY
                     LENAGQVFLTSADTERDFETKRPIRHFKVINGSVSA"
BASE COUNT          782 a          886 c          776 g         1015 t
ORIGIN      
        1 tgcccgtcca ccagcatata gcgcagcgaa aagggttggg ccatacgaac gatcgtatcg
       61 tacacggccg tgtcgccgtg cggatggtat ttaccaagca cttcaccgac gatacgcgcc
      121 gattttttat acggcttatt gtaagacatg ccgagctcat tcataccaaa cagcacgcgg
      181 cgatgcaccg gtttcaatcc gtcgcgcaca tccggaatcg cacgggacac aatgacggac
      241 atcgaataat cgatgtaaca ttcccgcatc tcgtcttcga tattacgcgg aacaagtttt
      301 tctatagtgg acatttcgtt tagtagctcc tgctatcgtt atataataaa atttttatac
      361 atccaaattg cgcacgtatt tcgcattgtc ttcgataaat ttacggcgcg gttcgacggc
      421 gtcacccatc aacgtagaaa acacgtggtc ggcggtcgcg gcatcttcga tcgtcacctg
      481 catcataacg cgcttgtccg gatccatcgt cgtggaccaa agttgctcgg ggttcatttc
      541 acccaaacct ttgtaccgct gaatattggt atcttcttct ttggcgcccc actgtttgag
      601 catggcggct ttctcttcat catcaaacgc gtatttttct tctttacctt ttttcaagcg
      661 atatagcggc ggctgggcga tatataagtt accgttttcg accaattggc gcatgtgacg
      721 gaagaaaaac gtgagcagca gcgtgcgaat atgcgcgccg tccacgtcgg catcggtcat
      781 gataatgact ttgccgtagc gcagattggc gagattaaaa tcatcggtac cgatgcccgt
      841 gccgatcgcg cttacgatgg tcagaatttc ctcattggaa agcattttat cgaggcgcgc
      901 tttttcgacg ttgaggattt tacctttcag cgggagaatg gcctgaaaac gacgatcacg
      961 gccttgtttg gcggagccgc cggcggaatc accctcgacg agataaattt cacaatattg
     1021 cggatcgctg atggaacaat cggctaattt tccgggaagg ccggatgact ccagcatgtt
     1081 tttcttacgc gtaagatcac gcgctttacg tgcagcttcg cgggcacgcg cggcctgaag
     1141 acacttatca ataatctgtt tggcgacacc gggactcttt tcgagaaact ccatcaggcc
     1201 ttcgccgaca gcttgttcca cgataccctt aacttcgcta ttacccagtt tggttttggt
     1261 ctgcccttca aattgcggct cggcgatctt aacgctgata atcgctgtca ggccttcgcg
     1321 ggtatcgtcg ccctgaatcg agatgccttc tttttctttg actaaattgt tttttgtcgc
     1381 atagttattc agcgtacgcg taagcgccgt acgaaaaccg gaaaggtgtg taccgccttc
     1441 gaccgtattg atgttattga cgtagctgaa tacgttttcg ttgtaggaat cgttgtactg
     1501 aaatgcaatc tcgaccggca cgttttcttt ttcggtttcg atatagatcg gttttttcat
     1561 caaggccgca cgcgattcgt caagatattg gacgaattgc gaaataccgc cttcgtagtg
     1621 gtattcttcg cgacgatttt tttcgcgttt atcttccagc gtgattttca gattgcggtt
     1681 gagaaatgcc agttcgcgca tacgtgcggc caatgtgtcg aaagtgtaca cgattttttt
     1741 gaaaatcgtg ctgtcggcca taaacgtcgt tgtcgtaccg ctttctttgg acttgcccac
     1801 ttcgtcaact ttggtcgtcg aacggccggc tttatatttc tgacgataca tctttccgtt
     1861 tcggcgcact tcgacttcgc accattcgga aagcgcattg acgacggacg cgccgacacc
     1921 gtgcaaaccg ccggagactt tgtacgtatt tttgtcaaac ttaccgccgg cgccgagcac
     1981 ggtcatgacg acttcgaccg caggtttctt ttccgtcggg tgcatgtcca cgggaatacc
     2041 gcggccattg tccacgacag tgatcgaatt atcttcattg atggaaacat tgatctccgt
     2101 acaataaccg gccattgcct cgtcaatgga gttatcaaca atctcgtaca ccaaatggtg
     2161 caaaccgcgg gtactgacat cgccgatata catcgcggga cgccgacgaa catgctctag
     2221 tccttcgaga acctgaatat tttcagcgtc gtattttgcg ccggtttttt tctgttcttt
     2281 ttctgccatg aatcctttag tccttttaat ctatgattat gatttatttt tctgacggtt
     2341 cttatttcaa tctttctaac acgcccttgc gcttaacgat attcttgtaa tcccattgta
     2401 tcgttttggc tttttttaac caacgcgcca agtctttttt gataacctga tccgcgcttg
     2461 taaaacgttt ttccgctgct ttgaaacggc cttcattttg cagaccgggt tcatcaaatg
     2521 actgtccgct ccaaaaaagc aatcgaatgc agcttttcag cttgctgtat ccgacaatcg
     2581 gattcccatc aagaaaccaa accggatgcg catgccatac tttgttttcg gcctccggca
     2641 atccgcggct aatctcagcg tacagtgttt cgcagatcag ccgatcttcg tcggagagtt
     2701 ggccgtgata ggctttaaca tctttatgca taacgcatta tttcaaaatt accggaaaac
     2761 tatttcgcgt actatttttt cacctaacgc ggaattgatc cgttcgataa tatttctttt
     2821 ttgataaaaa agttcctgcc gccacacgct attgaccacc tccaaaaaaa gcagtccgtc
     2881 ttcgatcttg accgctttgg cgcgctgagt aaatgccgtt cccatgaggc gttcaaactg
     2941 ctccagaacg acctgctcgt gtacacgatc cgcgatctta taacgtttga gtaacttatc
     3001 cagcgcttca ccgagcggcg tcgcatctgt ttttttgggt ttcacgcact tacacttccg
     3061 tttatcactt taaaatgccg gatcggtctt ttggtttcaa aatcacgctc cgtatcggcg
     3121 cttgttaaaa aaacctgacc ggcgttttca agataccgga taagattatg acgtcttgtc
     3181 caatccagtt ccgaaaaaac atcatccagc aaaagcagag gttgcaactc ccggcgttcg
     3241 gccatatgta gaaactcggc cagtttcaac gccagcgcca tggttttgag ttgtccttgc
     3301 gaaccaaact cacgtatcgc tttgtcgtta agtaaaagca aaagttcgtc tttgtgcggc
     3361 ccgatcagcg aagtttggcg catcatttcg cgatcgcggt tttcagcgat tgttttagcc
     3421 atcgcctccg cgatactttg atcgtcgggc aaagtttcg
//
LOCUS       DAOQNQ010000324         3446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_13384_length_3446_cov_4.910351, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000324 DAOQNQ010000000
VERSION     DAOQNQ010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13384_length_3446_cov_4.910351"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..736
                     /locus_tag="PK754_14240"
     CDS             71..736
                     /locus_tag="PK754_14240"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNB10710.1"
                     /translation="MKNKSTEDYLKHIFWLSRMRDKITTKAIAERMGISSASVTDMIK
                     KLTHHGYLDYTPYYGVRLTTKGDKLALKVIRRHRLLEAFLVKALGYSWEAVHDEAERL
                     EHVISEEMEHRLDEFLGHPRYDPHGDPIPTHEGKMEEIHHPSLAELSVGDTARIMQVR
                     DSEELLKYMKRIRLSLNSRIRICAKESYDNSMRLRIDGKEEHTLSHEVTSNIFVKKEK
                     EKK"
     gene            733..981
                     /locus_tag="PK754_14245"
     CDS             733..981
                     /locus_tag="PK754_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNB10711.1"
                     /translation="MKDHTTTPKITLDSVPIGASVRIAEMHIHGMARRRLLDFGFLRD
                     EPIEVVSRSPFGDPTAYRVKGTLIALRREEAKKIEVVL"
     gene            986..3175
                     /gene="feoB"
                     /locus_tag="PK754_14250"
     CDS             986..3175
                     /gene="feoB"
                     /locus_tag="PK754_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNB10712.1"
                     /translation="MSIVKENNKKQKSIQTPVFLNMSKTAHEMVIALAGNPNTGKSTV
                     FNALTGLNQHTGNWPGKTVKRMEGHFTYGERRYRLVDLPGTYSLLSASTDEEIARNFV
                     LFGKPDVTVVVVDATNLERNLNLALQIMQITDRVIVCVNLIDEAERKGIRIDAARLQE
                     DLGIPVVLTAARSSQGLDVLQETIAQLSKNLIPVKPFRINYPESLRETVDILSQKIHA
                     YDPKIPNPSWMAVRLLDKDQGIREALLSGHWHTRLLSNDIPVPALTNPDHPGVYEIRS
                     IIDEADSASEKIQTVLHDSLVESLFYDAKRIAGRAVEQTRITRLWENALDRWLTSRFV
                     GIPLMLLVLAVVFYLTIAGANVPSAMLADLLFSVEDFLAAGMDQLGAPWWLTGFLIHG
                     VFRGLAWVVSVMLPPMAIFFPMFTILEDFGYLPRVAFSLDRLFKWSGAHGKQALAMGM
                     GFGCNAAGVIATRIIDSPRERLIAILTNNFVPCNGRWPTLIMLASLFVAASLPAGVAS
                     IVAASAIVGITLFGILVTLLVAKFLSTATSLKGESSFFQIELPPYRRPQFFRILYTSL
                     IDRTGLVLWRAVVMAAPAGGLCWILANVQYGGVSLFAHGAAWLDPFGQSIGLDGIILL
                     AYIIAIPANEIIVPTIIMGYMAAGQMTELDSDIELKALFIQNGWTSLTAISLMFFSLL
                     HYPCSTTTLTIWKETRSVKWTLVSNIMPLAIALAVCFLIAQIGYWLG"
BASE COUNT          888 a          716 c          855 g          987 t
ORIGIN      
        1 aaataaatct tgacattatt taggaattcg tatataatag tttagttgag cctaatcttt
       61 tttattggtt atgaagaata aaagtacgga agactactta aagcacatat tttggctgag
      121 tcgtatgcgg gacaaaataa cgaccaaagc catagctgaa cgaatgggta tttcgtccgc
      181 gtccgtgacc gatatgatca aaaaactgac gcatcacggt tatttggatt atacgccgta
      241 ttacggagtc cgtttgacaa ccaagggcga caaactggcg cttaaagtga ttcgccgcca
      301 ccgtcttctg gaagcctttc ttgtgaaggc attaggttat agttgggaag ccgtacatga
      361 tgaggcggaa cggcttgaac atgtcatttc agaggaaatg gaacatcgtc tggatgaatt
      421 tctcggtcat ccgcgttacg acccgcacgg agatccgatc ccgacccatg aaggcaagat
      481 ggaagaaata catcacccgt cactggccga gctttccgtc ggtgacacgg cgcgtatcat
      541 gcaagtacgt gattccgaag aattgttgaa atacatgaag cggatccgcc tgtcgttgaa
      601 ttcgcgcatt cgtatttgtg ccaaagaaag ttatgataac tcgatgcgtc tgcgtattga
      661 cggtaaagaa gaacatacgt tgagtcacga agtgaccagc aatatttttg taaaaaaaga
      721 aaaagaaaaa aaatgaaaga tcacacgaca acccccaaaa tcacactgga cagcgtcccg
      781 atcggcgcgt cggtacgtat tgccgaaatg catatacatg gtatggcacg tcgtaggctt
      841 ctggattttg gttttttgcg tgatgagcct attgaggtcg tgagccgaag ccctttcggt
      901 gatccgacag cgtaccgtgt caaaggcacg ttgatcgcgt tgcgccggga agaagctaaa
      961 aaaatagagg ttgtgttata agactatgag cattgttaaa gaaaataata aaaaacagaa
     1021 aagcattcag actcccgttt ttcttaatat gtcaaagacg gcgcatgaga tggtcattgc
     1081 gttggccggc aatcccaata ccggaaaaag tacggttttt aatgcattaa ccgggctaaa
     1141 tcagcatacc ggtaattggc ccggaaaaac ggtcaagcgt atggaaggtc attttaccta
     1201 tggcgaacgc cgttaccgcc tggtagactt gccgggtacg tattcattgc tttcggcttc
     1261 gacggacgaa gagatcgcgc gcaattttgt gttattcggt aaaccggatg tgaccgtcgt
     1321 cgtcgtggat gctaccaatc tggaacgcaa tctcaacctg gcgctgcaaa tcatgcagat
     1381 cacggatcgc gtcatcgtat gtgtcaatct gattgacgaa gccgaacgta aaggtattcg
     1441 cattgatgcc gcgcgcttgc aggaagattt aggaataccg gtggtgctca cggcggcacg
     1501 aagcagtcaa gggctcgatg ttttacagga aaccattgct caactgagta aaaacctcat
     1561 accggtgaaa ccgtttcgaa taaattatcc ggaatcgttg agagagacag tggatatttt
     1621 gagtcagaaa attcatgcgt atgatccgaa aattccaaac ccttcctgga tggccgttcg
     1681 tttgttagac aaagatcagg gtatccgcga agctttatta tccggccatt ggcatacgcg
     1741 tcttctgagt aacgatatac ctgtccctgc gcttacaaac cctgatcatc cgggcgtata
     1801 tgaaatcaga agtatcattg acgaagcgga tagcgcatcc gaaaaaattc aaaccgtact
     1861 gcacgacagt cttgtcgagt cgctatttta tgatgcaaaa cgaattgccg gacgcgcggt
     1921 ggaacaaaca cgaataacgc ggctttggga aaacgcctta gatcgttggt tgacttcgcg
     1981 ttttgtaggt attccgttga tgctgttggt attggccgtg gtgttttatc tcaccattgc
     2041 cggagctaat gtaccttcgg cgatgttggc cgatctgttg ttttccgtag aagatttttt
     2101 agcggccggt atggatcaac ttggagcgcc gtggtggttg accggttttt tgattcatgg
     2161 tgtatttcgc ggattggctt gggtagtgtc tgttatgtta ccaccgatgg cgattttttt
     2221 tcctatgttt accatactcg aagatttcgg atatctgccg cgtgtggcgt ttagtttgga
     2281 tcgtttattc aaatggtcgg gtgcacatgg caaacaggcg ttggctatgg gtatgggttt
     2341 cggctgcaat gcggcgggtg tgatcgcgac acggatcatt gattctccga gagagcggtt
     2401 gatcgctata ctcaccaata attttgtccc ttgcaacggg cgctggccga cgttgattat
     2461 gctggcctcg ctttttgtag cggcttcgtt accggctggg gtggcttcga ttgtcgcagc
     2521 atcggcgatc gtaggcatta ctttgtttgg gattttagtt acgcttttgg tggccaaatt
     2581 tctgagtacg gcgacaagcc tcaaaggcga atcttctttt tttcagattg agttaccgcc
     2641 gtatcgtcgt ccgcagtttt tcaggatttt atatacctca ctgattgatc gtaccggttt
     2701 ggttttgtgg cgcgcggtgg tcatggcggc tccggccgga ggcctatgct ggattttggc
     2761 taatgtgcag tatggtggtg tgtcgctttt tgcgcatggc gcagcgtggc tggatccttt
     2821 tggccaatcc atcgggctcg acggcatcat cttacttgca tacattatcg ctataccggc
     2881 caatgaaata atcgttccca cgatcataat gggttatatg gccgccgggc aaatgacaga
     2941 gttggattcg gatatcgaac tgaaagcctt atttattcaa aacggatgga cgtccttaac
     3001 ggccatatcg ttgatgtttt tttcactcct tcactaccca tgcagtacga cgacactcac
     3061 gatttggaaa gaaacgcgca gtgtcaagtg gaccttggtt tccaatatca tgcctcttgc
     3121 tattgctttg gccgtatgtt tccttatcgc ccaaataggg tattggttgg gataatggcg
     3181 tatttttgca aaatcggtaa agtcaataaa taaaaagtag cattattaat ccttcacgta
     3241 tatctacgta ttttatacta aatcctgaat ttttattaac atcttgtttt tttaaacaaa
     3301 acgtgaccgt tttgtcaaaa gcttgtaaaa acatgctgta tgcccgtgtc atttaggtcg
     3361 gcaaaattac ttacaaaaat atttgacaat gtgagctaat tattttaata ttatgacatt
     3421 atgaataaaa gtgtatccgt caattc
//
LOCUS       DAOQNQ010000325         3445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_13390_length_3445_cov_5.512684, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000325 DAOQNQ010000000
VERSION     DAOQNQ010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13390_length_3445_cov_5.512684"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PK754_14255"
     CDS             complement(<1..415)
                     /locus_tag="PK754_14255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB10713.1"
                     /translation="MSSIKDILAVDDEPVVLTAISKTCTSERLKVDCAESAAEALKRM
                     ESQDYRLIISDIKMPEMDGFQLLAEVKKRGITSGVIISTGYATVENAVHALSEGAIDF
                     IPKPFTADELLSALYRGLRYGTIVSSENKSGNILPY"
     gene            complement(412..2277)
                     /locus_tag="PK754_14260"
     CDS             complement(412..2277)
                     /locus_tag="PK754_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB10714.1"
                     /translation="MPAWLDKIGFRLILAVGVTAILIIGVFSYFNIRSHTEVLLAEVE
                     RRANQLSETVKYSTRHDMLFNQRERIHRQIRDIGEQPGINRIRLLNKTGEIVYSSKPD
                     DIGKMVDKNAESCFVCHSDQKPIERLTISERTRIFRLHPDSVRIMGIINPIYNEASCW
                     EASCHVHEPEQKVLGVLDVTIPLADVDAQVQSSRLEMTIFALVSFVALSCIIGVFVYR
                     WVEIPVHTMVTATHEIAGGNLNHALPENRSDELGQLARAFNHMTRKMAEARLQLFQSD
                     KMASLGRLAAGVAHEINNPLTGVLTYSSYLMKRTASQPDIQEDLKVIVRETLRSREIV
                     KSLLDFARQSVPKKSAADICEIIRRSLSVTENQLRLHRVKVETNFNEDMPHVIVDSNQ
                     MQQVFINLLVNAADAMSESGGTIHIHLARINLSPYGTTQIRKALCNKNHNLMSPDIKI
                     NGLPTIRLKAKSEGAEGFLYLDPIYGSHQHEYGILSGNILSLQCPQCLTSVINESVRC
                     PKCNALTYSFEVAGKGVFRGCTRNGCDWQQWPIMDEAGVREFVEIKVRDNGIGIPPED
                     MSRIFEPFFTTKGQKGTGLGLSVIWGIIDNHDGKITVESIPGQGTTFTIRIPVKS"
     gene            complement(2280..3419)
                     /locus_tag="PK754_14265"
     CDS             complement(2280..3419)
                     /locus_tag="PK754_14265"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNB10715.1"
                     /translation="MKMNSFLILMFWTVMVFGQKNNPLHNKTNLPCKTCHTCEFPTIK
                     DPCLIDCPRENLITIRASVYSAPEFIQLNRLSDRYAGVRFSHKRHAEMYDMSGGCGGC
                     HHYNTSGPVQSCAACHALKNENKPDKPESTPGLQAAYHRQCITCHKTWSAEAGCNSCH
                     DLKKSVVSQSEKNKKDSLLAISHRTRKEPEKIVYETDTDKGRIATFYHKDHTQLFGVS
                     CGQCHSQLSCVKCHDQKRSMNEPLVQNVINKTEEEKHAACFNCHKDDNCTKCHSNKEL
                     QPFDHGTRTGWPLANYHTRLICSNCHGANKNFKGLSRECNTCHTAWTMTTFRHAVTGL
                     VLSEDHLELSCKDCHQKNKYTTKPVCMPCHDDKTYPNDLPGRKLW"
BASE COUNT          873 a          822 c          751 g          999 t
ORIGIN      
        1 cgtaaggtaa aatattcccc gatttatttt ccgatgaaac gatcgtacca taacgtaacc
       61 cgcgatacaa agccgataat agttcgtccg cagtaaacgg tttggggata aaatcaattg
      121 caccttcaga caacgcatga acggcattct cgacggtggc gtatcctgtg gaaataatta
      181 cgcctgaagt aataccgcgt ttttttactt ccgccaaaag ttgaaatccg tccatttcgg
      241 gcattttgat atcactgatg atcaggcgat aatcctgtga ctccatacgc tttaacgcct
      301 cggccgccga ttcggcacag tccactttca gtcgctcgga tgtacatgtt ttgctgatcg
      361 ccgtcaaaac gacgggttca tcatcgacag ccaatatgtc tttgatactg ctcatgattt
      421 taccggtata cgtatcgtaa acgtcgttcc ctgaccggga atactttcga ccgttatttt
      481 cccgtcgtgg ttatctataa tgccccaaat caccgatagg ccaagcccgg ttcctttttg
      541 ccctttggta gtaaaaaacg gttcaaaaat tcgactcata tcttcgggtg gaattccgat
      601 accgttatcg cgcactttga tttcgacgaa ttcacggaca ccggcttcat ccatgatcgg
      661 ccactgctgc caatcgcatc cgtttcgcgt gcatccccta aagacacctt taccggcgac
      721 ctcgaaacta tatgtcaacg cattgcattt agggcaacgt acggattcat tgatgacgga
      781 cgtgaggcat tgcgggcatt gcaaactcag tatatttccc gacaaaatgc catattcgtg
      841 ttgatggctt ccgtaaatcg gatcaaggta taaaaaacct tccgcccctt cgcttttagc
      901 cttgaggcgt atggtcggta acccgttgat cttaatatca ggactcatca ggttgtgatt
      961 tttattgcaa agcgctttac gaatttgcgt cgtaccgtaa ggcgaaagat tgattcgtgc
     1021 gagatgaata tggatcgtac ccccggattc gctcatcgcg tcagccgcat tgaccaaaag
     1081 gttgataaat acctgctgca tttgattgct atccacaatg acatgcggca tatcctcatt
     1141 aaagtttgtt tcgactttga cgcgatgcag acgcaattga ttttcggtga cactcaagga
     1201 ccggcgaatg atctcacata tatccgcagc actttttttg ggaacggact gccgtgcaaa
     1261 atctaaaaga cttttgacga tttcgcggct gcgaagggtt tcacggacga tgactttgag
     1321 gtcttcctgt atatccggtt gtgaagccgt gcgcttcatc aaataactgc tgtatgtcaa
     1381 aacaccggtg agaggattat tgatctcatg cgcgacaccg gcggccaatc gtccgagcga
     1441 agccatttta tcggattgga aaagctgtaa ccgtgcttcc gccatttttc gtgtcatgtg
     1501 attaaatgcc cgagccaatt ggcctaattc gtcgcttcgg ttttcaggta acgcatgatt
     1561 gagattcccg ccggcgattt catgcgtcgc cgtaaccatc gtgtgcaccg ggatttctac
     1621 ccatcggtat acaaaaacgc cgatgataca actgagcgcg acaaaggata ccaaagcaaa
     1681 aatcgtcatc tccaatcggc ttgactgaac ttgagcgtcc acatcagcta aaggaatcgt
     1741 cacatccaaa acaccgagca ctttctgttc gggttcatgc acatggcacg acgcttccca
     1801 gcacgacgct tcgttgtata tcggattgat aatacccata atccgtaccg aatccggatg
     1861 taatcggaag atacgcgtac gttcactgat cgtaagacgt tcgatcggtt tttgatccga
     1921 atgacacaca aaacaacttt ccgcattttt atctaccatc ttacctatat catccggctt
     1981 ggatgaataa actatttcac cggttttatt aagcagtcgg atgcggttaa tgccgggttg
     2041 ttcgccaatg tcccgaatct ggcgatgaat gcgttcacgt tgattaaaaa gcatatcatg
     2101 ccgcgtgctg tacttgaccg tttcactcag ttgattggcg cggcgttcta cttcggcgag
     2161 cagtacttcg gtgtgcgaac gaatattgaa atatgaaaaa actccgatga tgagtatcgc
     2221 cgtcactccc accgccaaaa tgagtctgaa tcctatttta tccagccatg ccggcatatt
     2281 taccacaact ttctcccggg taaatcattg ggatacgttt tatcatcatg acaaggcatg
     2341 catacaggtt ttgtggtata tttatttttt tgatgacaat ctttgcacga caattccaaa
     2401 tgatcttcac tcaaaaccaa tcccgtgacc gcatgacgaa atgtcgtcat tgtccacgcc
     2461 gtgtggcatg tgttgcattc gcgcgatagg cctttaaagt ttttgtttgc cccgtgacaa
     2521 ttcgaacaaa ttaatcgtgt atgataattg gccaagggcc aacccgtgcg tgttccgtga
     2581 tcaaacggtt gcaattcttt attgctatga catttggtac agttgtcatc tttatgacaa
     2641 ttaaaacaag cggcatgttt ttcttcttca gttttattta tgacgttttg tacgagcggc
     2701 tcgttcatcg aacgtttctg atcatggcac ttcacgcatg agagctgtga atgacactgc
     2761 ccgcaagaca caccaaataa ctgagtatga tctttgtgat aaaatgtagc tatgcgccct
     2821 ttatccgtat cggtttcata cacgattttc tcaggctctt tgcgggtacg atgacttatg
     2881 gctaggagtg aatctttttt atttttttca ctttggctta cgactgactt tttcaaatca
     2941 tggcaggaat tgcaccctgc ttccgcactc cacgttttat ggcacgtaat gcactggcga
     3001 tgataagcgg cttgtaaacc cggggtagat tccggtttat cgggtttgtt ttcatttttt
     3061 aaagcatggc aggccgcaca cgactgtaca gggccggatg tattgtagtg atggcaaccg
     3121 ccacacccgc ccgacatgtc gtacatttca gcatgccttt tgtgagaaaa acgcacaccg
     3181 gcgtatcggt cactgagccg attgagttga ataaattcag gcgccgaata tacggacgca
     3241 cgaatagtga tcaggttttc acgaggacag tcaatgaggc atggatcttt gattgtcgga
     3301 aattcacacg tatggcatgt tttgcagggt aagttcgttt tgttatgcaa cggattgttt
     3361 ttttgcccaa acaccatcac agtccaaaac atcaatatga ggaaactatt cattttcata
     3421 acggcaaccc tttaatcgca tattt
//
LOCUS       DAOQNQ010000326         3425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_13505_length_3425_cov_5.660237, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000326 DAOQNQ010000000
VERSION     DAOQNQ010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13505_length_3425_cov_5.660237"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1734
                     /locus_tag="PK754_14270"
     CDS             <1..1734
                     /locus_tag="PK754_14270"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNB10716.1"
                     /translation="KNLKSKSGVPIQLWYYPDQADRIEPTYRHTVTAMDFLEKEIGIP
                     YPWGVFANIPVSDFVTGAMENTTAVLFGDFWYVDRRAFNDDNYVDTDFHEQAHQWFGD
                     MITMYDWKHLWLQESFATYYGKLGMYHVYGEDMYQWKRREEQNQAINASENNRLPITH
                     TQAGTARFYPKGSAVIDMMNHVWGRDAVRRAVYHFLSHNRFKNVETYGLMRSFMDTLG
                     VAPQWFFDQWIYRGGEPHYKISYLDLRRTSGRQTEIAVQQTHKTDELTGLFKMPIDFE
                     VHYADGTKDSVREWIENTVTTVVVPNTHDKEIAFVLFDPGSWVTKSYEFEKHFDELAA
                     QALQAPNMIDRYDALVMLRAYDLPSKRELLIRVYDKETFHALKAEVAEQLVNDEDEAG
                     RAVIRRAITDAWPEVRKAVINNVGRIPQTMLSDFEKLLSDSSYNVVRVALARLINQYP
                     VHADRYLDMTKDQYGLSNEIKMDWLEQKVNRGDKDALMQIVEYSSNAYEFGTRRNAMS
                     ALKRLNYLDDKVIGNLLDAALNANESLSNRAKNILDYFTDQSPYRRRIQSYYRSHTWE
                     DWQKEILQALL"
     gene            1901..2386
                     /locus_tag="PK754_14275"
     CDS             1901..2386
                     /locus_tag="PK754_14275"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB10717.1"
                     /translation="MKEFLEDFEETVKKSVKRLSKLSDQESGIAKTPDKWSAKQIIGH
                     LIDSAANNHQRFVRAQFGDDLVFPGYDQEKWVKVQRYDLSDWNALINLWNSYNFHIIH
                     VVKQIPESDLFKSRSKHNLDQIAWKTVPVSESVTLDYFIRDYVGHLKNHLDQIWSQYK
                     V"
     gene            complement(2477..>3425)
                     /locus_tag="PK754_14280"
     CDS             complement(2477..>3425)
                     /locus_tag="PK754_14280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB10718.1"
                     /translation="FDRTLKYFNGEEGAFNLTVSVFQNSLIRNRGSIVIASTHILQTP
                     PIVKIAKGTDTTQVPVATLSGAKVYSGAFNLSEGSYKLITRGVSHKGPDSTKLRSFNV
                     SLLKPQTEALITVPDASATLAIEKQNFTGYVMATTEDKGAEKIFHFGTPDMTALARIA
                     IKVPGISVETAEKTLIYRQEGNEWKWVPTVFVPGQDVLEASVERFGTYKTVYDPQTSA
                     STEILRSFELKQNYPNPFNPTTTITYDIPSTMKVSLVVYNILGQKIKTLWDGVRTAGR
                     YTQIWNGRNEKGQLVTSGVYLYQIKAGNKTSVKRMLLIK"
BASE COUNT          946 a          731 c          810 g          938 t
ORIGIN      
        1 aaaaacctca aatccaaaag cggcgttccg attcagttgt ggtattatcc cgatcaggcc
       61 gatcgtatcg aaccgacgta ccgtcatacc gttacggcga tggattttct agaaaaagaa
      121 atcggtattc cttatccctg gggtgttttt gccaatatac ccgtttccga cttcgtgacg
      181 ggtgcgatgg aaaatacaac ggctgtttta ttcggtgatt tttggtatgt ggaccgtcgt
      241 gcctttaatg atgataacta cgtagatacc gattttcatg aacaggcgca tcagtggttt
      301 ggcgatatga tcacgatgta tgactggaaa catctttggc ttcaggaaag ttttgcgacg
      361 tactacggca aactcggtat gtatcacgtg tacggcgaag acatgtatca gtggaaacgc
      421 cgtgaagaac aaaatcaggc gatcaacgca tcggaaaaca atcgtcttcc gatcacgcat
      481 acgcaagccg gcacggcacg cttttatccc aaagggtcgg ccgtcataga tatgatgaat
      541 catgtctggg gtcgtgatgc cgtacgccgc gccgtatatc attttttaag ccacaaccgt
      601 tttaaaaatg tggaaaccta cggcctgatg cgttccttta tggatacgct gggcgtggcg
      661 ccgcagtggt tttttgatca atggatttat cgcggcggtg agccgcatta caaaatcagt
      721 tatcttgatc tgcgccgtac ttcaggtcgt cagacggaaa tcgccgtgca acaaacgcat
      781 aagaccgacg agctgacggg gctttttaaa atgccgattg attttgaagt gcactatgct
      841 gacggtacca aagatagcgt gcgcgaatgg atcgaaaaca cggtgaccac cgtcgtagta
      901 cccaataccc atgataaaga gatcgccttt gtgctttttg atccgggcag ttgggttacc
      961 aagtcctatg aatttgaaaa acattttgat gaactggcgg cgcaggcact acaggcgcct
     1021 aatatgatcg atcgttacga tgcgctcgtc atgctacgtg cctacgatct gccatccaaa
     1081 cgcgaactcc tgatacgcgt ttacgataaa gaaacatttc acgcactcaa agccgaagtc
     1141 gccgaacagt tagtcaatga tgaagatgaa gccggcagag cggttattcg tcgcgcgatc
     1201 accgatgctt ggccggaagt gcgtaaggcg gtgatcaata atgtcggtcg cattccgcaa
     1261 accatgcttt ccgattttga aaaactattg tcggacagtt cgtacaacgt cgtacgtgtc
     1321 gcgctggcac gcctgatcaa tcaatatccg gtccatgccg atcgttatct ggatatgacc
     1381 aaagatcaat acgggttatc caacgaaatc aaaatggatt ggctggagca aaaagtcaat
     1441 cgcggcgata aagatgcgtt gatgcagatc gtggaataca gcagcaatgc atacgaattt
     1501 ggtacgcggc gcaatgcgat gagtgcgctc aagcggctca attatttaga cgataaagtg
     1561 atcggaaatc ttttggatgc tgcgctcaat gccaacgaat cgctgagtaa ccgtgcgaaa
     1621 aatattttgg attattttac ggatcagagc ccgtatcgtc gccgcatcca atcctactac
     1681 cgttcacata catgggaaga ctggcaaaag gagattttac aagcgttgtt gtaaatgtga
     1741 tcattggcgc ggtcgctttg atgggcaatg agtgtgaaga aaagttcata aatcgaactc
     1801 atgaatagta cgatcataac gtgtcaggtt tcgaatttat tttttgatcg ggaactgcac
     1861 gcgtaaatgc gcatcaaatc atcggagtag aatatccaaa atgaaggagt ttcttgaaga
     1921 ttttgaagag accgtgaaaa aatcggtcaa acgtctttca aaactgagcg atcaagaaag
     1981 cggtattgcc aaaacgcccg ataaatggtc ggccaaacag attattggac acctgattga
     2041 ttctgctgct aataatcatc aacgttttgt acgagcccaa ttcggtgatg acttagtatt
     2101 tccgggttac gatcaggaaa aatgggtgaa agttcagcgg tatgatctgt cggattggaa
     2161 tgcgttgatt aatctttgga attcttacaa ctttcatatc atacatgtgg tgaaacagat
     2221 accggaatct gaccttttca agagccggtc aaaacataac cttgatcaaa tagcttggaa
     2281 aacagtgccg gtaagtgaat ccgtcaccct cgattatttt attagagatt atgtagggca
     2341 tttgaaaaat catcttgatc agatttggtc gcaatacaag gtgtaaacat cagatcagcc
     2401 ggcgagtatt ctctgtatga aaacctgata gttgatttga catttgacgg acagaaatac
     2461 ggatctctcg catgaattat ttgatcaaaa gcatacgttt gacgctggtt ttatttccgg
     2521 ccttgatttg atacaaatac actccgcttg ttacaagctg acccttttca ttacgaccgt
     2581 tccatatttg tgtataacgt ccggccgtac gtacaccatc ccataacgtt tttatttttt
     2641 gaccgaggat attataaacc accaatgaaa ctttcatcgt ggatgggatg tcgtacgtga
     2701 tcgtagtcgt cgggttaaac ggattgggat aattttgctt aagttcaaat gaacgaagaa
     2761 tttccgttga tgccgacgtc tgcggatcgt atacggtctt gtatgtaccg aatcgttcga
     2821 cggacgcttc caaaacatct tggcccggta caaataccgt aggaacccat ttccattcgt
     2881 ttccctcctg acgatagatc aacgtttttt cagcggtttc aacggagatg ccgggaactt
     2941 taatggcaat tcgcgccagt gcggtcatgt cgggtgtacc aaaatgaaat attttttccg
     3001 cacccttatc ttctgttgtc gccatgacgt aaccggtaaa gttttgtttt tcgatagcca
     3061 atgttgcgga agcatcagga actgtaatca gagcttctgt ttgcggtttg agtaaagaga
     3121 cattaaagga tcgcagtttt gtagaatcgg gtcctttatg cgacacgccg cgtgtaatga
     3181 gtttataact accttcggat aaattaaaag cgccactata tactttagcg ccgctcaaag
     3241 ttgccacggg aacctgtgtc gtatccgtac ctttggcgat tttgacgatc ggcggggttt
     3301 gtaaaatatg cgtggatgct atgacgatac ttccgcgatt acggatcagc gaattttgaa
     3361 acactgaaac cgtcaaatta aacgccccct cttcgccatt aaaatatttg agcgtgcgat
     3421 caaat
//
LOCUS       DAOQNQ010000327         3420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_13543_length_3420_cov_6.228529, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000327 DAOQNQ010000000
VERSION     DAOQNQ010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13543_length_3420_cov_6.228529"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3420)
                     /locus_tag="PK754_14285"
     CDS             complement(<1..>3420)
                     /locus_tag="PK754_14285"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HNB10719.1"
                     /translation="IGSDNSTIPNNYHGIALNAGGGSQIIGNVISNHPQSGILIAGSS
                     YNKVQDNLIGTDASGIIGYPNLIGISLIGGSKYNEIGDTLGVNQNVISGNTNTGILIT
                     DAGTDSNYVWGNLIGTDINGTSDLGNGSWGIALDNNSMHTAIGGMTSNHRNVISGNGN
                     SGILMQGFSAHNVVQGNYIGTDINGNVDLGNTQSGVFILNQAHDNIVGGTSPGAGNVI
                     SGNNNSGITIGNIGTRGQIIQGNLIGTNATGNAAISNGSGVVLNSGHANLVGGTVAGA
                     RNVISGNTNSGIEITNVGSDTNLVQGNFIGTDVNGTVPLGNGIGVTISEGGSGSPRLN
                     LIGGATAAERNIISASTNYGVWFINSSENKVQGNYIGTDVTGTQNLGNSSDAINLSNG
                     ASRDSIMGNTIAFSNTNAIAMLNSGTDSIIVHDNVIFGNGNGFYLQAGTQSDITPPQI
                     ISVGADSTVYGIASPNAFIQVYADSINQGRFKLGTTTANASGDWSLKVNIPAGTAITA
                     MQDSAQGTSAFSMPFAPQPEIVVYTLTGTYLDYGGIFSGNGQIDTVFVKNIGAGALDI
                     TSLSIENGSSQFTIVDTLPNNLTLTPGDSVKLVVRFLPDGFSSYADSVTIISNDADES
                     ITHVAVYGTGVSGILGSFVVNPDFGNVPVGDSSSVTEVKLFAVSGNVLISGMPLMLAG
                     GSFDIDSVSGPAFLGLTDTMRVFVKYHANVFGIERDTISVNHNGQNSSPLMVALLGTG
                     ISGSLAALPSNYDFGNVAVGDSVDTVIKLASNGGAVAVSGSALNGGGYSIVSSIGPST
                     LYSGDTLVYTIRYKPTTFTTTLDTLKITNNSVTSTYTVALSGTGVSGTLVALPSSYDF
                     GNVAVGDSMDAVIKLASNGGAVAVSGSALNGGGYTIVSESGPNVLFSGDTLVYTIRYK
                     PTTFTTTLDTLKLTNNSSASTYTVAFSGTGASGTLLASSVALNFGNVQVGQSAQQTIK
                     FYSQGGAVTVSSTLAGPAFQVVSATGPSTLYAGDTLSVTLQYNAGVFGIETDTLRVST
                     NATNGGMNIALDGIGVPGTLVALPSSYDFGNVAVGDSAQANIKLYATGGAVIVHGVTN
                     MSAYFEIGSMNLPDTLYAIAGDTLTIPVKYLADAFGTRADTLAVNSNSGLL"
BASE COUNT          933 a          904 c          723 g          860 t
ORIGIN      
        1 tcaaaagacc gctgttgctg ttcacagcca gtgtgtcggc acgtgtgcca aacgcatccg
       61 ctaaatactt caccggtatc gtgagcgtat ctccggctat cgcatagagc gtatccggca
      121 gattcatact accgatttca aaatacgcac tcatattcgt cacgccatgc acgatcaccg
      181 caccgcctgt agcataaagc ttgatgttcg cttgcgcgct atcacctaca gcaacattac
      241 caaaatcata acttgacggt aacgccacga gtgtacccgg cacaccaata ccatccagtg
      301 ctatattcat accgccgtta gtcgcattgg tgcttacacg cagcgtatcc gtttcgatgc
      361 cgaagacacc tgcattatac tgtagcgtta cactcagtgt atcgcccgca tataaggtcg
      421 aaggccctgt cgccgatact acctggaatg ccgggccggc cagcgtactg cttaccgtta
      481 cggcgccacc ctgactgtaa aacttaatcg tctgctgtgc actctgacca acctgcacat
      541 taccaaaatt cagcgctacg ctacttgcca gcagcgtgcc tgatgcacct gtacctgaaa
      601 atgctactgt atatgttgat gcggacgagt tattcgtaag cttcagtgtg tccagagttg
      661 tcgtaaacgt ggtcggttta taacgtatcg tatacaccaa cgtatcaccg ctgaaaagaa
      721 cattcggacc actctccgaa acaatcgtat aaccgccgcc attgagtgcg ctgccgctta
      781 ctgcgaccgc accaccattg ctggccagtt tgatcaccgc atccatacta tcacctacag
      841 ccacattgcc aaaatcataa cttgaaggca atgctaccag tgttcccgaa acgcctgtac
      901 ctgagagcgc gaccgtatat gtcgaagtta ccgagttatt tgtaatcttc agcgtatcaa
      961 gagtcgtcgt aaacgtcgtc ggtttatagc gtatcgtata taccagcgta tcacctgaat
     1021 acaatgttga tggaccgatc gaagatacga tcgaataacc gccgccattg agtgcacttc
     1081 cactcactgc aactgcacca ccattgctgg ccagcttgat caccgtatcc acgctatcgc
     1141 ctacggctac attgccaaaa tcataattgg acggcaaagc agctaaagat ccagagatac
     1201 ctgtgcctag aagcgctacc atcaacggag atgaattctg gccattgtga ttgaccgata
     1261 tcgtatcgcg ttcaattcca aacacattag catgatattt tacaaacaca cgcatagtgt
     1321 cagtcaaacc gaggaacgca ggaccgctaa cggagtcaat atcaaacgaa ccaccggcca
     1381 acatcaatgg cataccgctg atcagcacgt taccgctcac ggcaaacaat ttaacttccg
     1441 ttacggatga agagtcaccg acaggaacat taccaaagtc gggattgacc acaaatgaac
     1501 ccaatatacc ggaaacaccc gtcccataaa ctgcaacatg tgttattgat tcgtcggcgt
     1561 cattgcttat gatggtgacc gaatccgcat aactgctaaa tccatccggc aagaaacgaa
     1621 caaccagttt gacagaatca cccggagtta aagtgagatt attgggcaga gtatccacaa
     1681 tcgtaaattg ggagctcccg ttctcaattg aaagcgaagt aatatccaga gcgccggcgc
     1741 caatattttt tacaaatact gtatctatct gaccgttacc actaaatatt ccaccgtaat
     1801 ctaagtacgt gccggtcaaa gtatatacga cgatctccgg ctgtggtgca aatggcatcg
     1861 aaaatgccga ggtaccctgc gcactatcct gcatggccgt aatcgcagta cccgcaggaa
     1921 tatttacttt caatgaccag tcaccggatg cattggccgt cgtagtaccc agcttgaacc
     1981 ggccctgatt gatcgaatcg gcatacactt gaataaaggc attcggtgaa gcaataccat
     2041 acacggtcga gtcagcgcct acggatataa tctgtggcgg agtaatatcg ctttgagtac
     2101 cagcctgcaa ataaaaaccg tttccgtttc cgaaaatcac gttatcatga acgatgatac
     2161 tgtccgttcc tgaattaagc atcgcaattg cattggtatt gctaaaagca atcgtgtttc
     2221 ccatgatcga atcgcgcgaa gcaccgttgc tcagattgat tgcatcgctg ctattaccca
     2281 agttttgagt gcccgtgaca tccgtaccga tatagtttcc ttgaacttta ttttcagaac
     2341 tattgataaa ccaaacaccg tagttggtac tggctgaaat tatatttcgt tcagctgcgg
     2401 tagcgccacc gatcaaatta agtcgcggag aaccggaacc accttcagaa atcgtcacac
     2461 cgataccgtt gccgagtggc accgttccgt tgacatcagt cccgataaag ttaccctgta
     2521 ccaaattggt atctgagcct acgttcgtaa tttcgatacc gctgtttgtg ttaccggaaa
     2581 tgacatttcg tgctccggcc accgtaccgc cgaccagatt ggcatggccg ctattgagta
     2641 ctacgcccga tccgttggat atagctgcat tacccgtcgc attggttcca atcagattgc
     2701 cttgtatgat ttgacctctt gtgccaatat tgccgatggt aatacctgaa ttattgttac
     2761 cagatattac gttaccggct cccggcgatg tgccgccgac gatattatca tgcgcttgat
     2821 tgagaataaa cacgccactc tgcgtattgc caagatcaac attcccgttt atatcggtac
     2881 cgatatagtt accttgtacc acattatgcg cagaaaatcc ctgcatcaaa ataccgctgt
     2941 taccgttgcc ggaaataacg ttgcgatgat tggacgtcat accaccgatc gcggtatgca
     3001 tggaattatt atcaagtgca ataccccatg aaccgttgcc gagatcagac gtaccgttaa
     3061 tgtccgttcc gatgagattt ccccaaacgt aattggaatc tgtgcccgca tccgtaatga
     3121 gaataccggt attagtatta ccggagataa cgttttgatt tacgccaagg gtatcaccga
     3181 tctcgttgta cttcgaaccg ccgatcaggg aaatgccgat gaggttagga taaccgatga
     3241 taccggacgc atctgttccg atcaaattat cctgtacttt attataagaa ctaccggcaa
     3301 tcaatatacc cgactgcgga tgattggaaa tcacattacc gatgatctga cttccgccgc
     3361 cggcattcaa agcgataccg tgatagttat tggggatcgt agaattatcc gaaccgatac
//
LOCUS       DAOQNQ010000328         3401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_13670_length_3401_cov_5.503885, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000328 DAOQNQ010000000
VERSION     DAOQNQ010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13670_length_3401_cov_5.503885"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..926)
                     /gene="ispD"
                     /locus_tag="PK754_14290"
     CDS             complement(234..926)
                     /gene="ispD"
                     /locus_tag="PK754_14290"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNB10720.1"
                     /translation="MIKGKKVTAILPAAGIGKRMGVGSKKQFLEVHGKPILVHTLEKF
                     HHAIYVDEVILVLQNEWIPFVQEEIIGPYGFSRIHTLVPGGTERQDSVYEGLKAVEES
                     DIVLVHDAVRPCVRADKIDELIEITVEYSAAILAVRPKDTIKQQDANGFVARTIDRNT
                     LWNVQTPQAFQYSLLRSAFDKAYAENYYGTDEAMLIERIGHAVKIVEGHYDNIKITTP
                     EDLALAERFLKH"
     gene            complement(928..1794)
                     /locus_tag="PK754_14295"
     CDS             complement(928..1794)
                     /locus_tag="PK754_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB10721.1"
                     /translation="MKVDTKIGAYHLHPVETGRFALDGGAMFGVVPKNLWNKTNPADE
                     QNRITLAMRCLLLVSDDGKKILIDNGVGHKYNEKFASMYRIDHSVYTLERSLQKYDLT
                     PNDITDVILTHLHFDHAGGSTKMDEQSRIVAAFPNAKYYVQQKHYEWGEKASEKDRAS
                     FLKPDYEPLVQSGQLQLIEQTDGIFPNITFFIANGHSPYQQLPIIHDAQHTVFFCGDL
                     IPTTTHIAVPYVMAYDNQPLVTIAEKKDILTRAFENRWILFFEHDPETAMSGVRRDEK
                     GFRMGDIVAVKG"
     gene            complement(1791..2327)
                     /locus_tag="PK754_14300"
     CDS             complement(1791..2327)
                     /locus_tag="PK754_14300"
                     /inference="COORDINATES: protein motif:HMM:NF022586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HNB10722.1"
                     /translation="MSETIAPVFKPADPSPVKRLVSKRDIGISAFELENGNVMLEATF
                     LDPYHLLRLNIQIDPHTRYIVSSKMEMANHPHSACPGVTEKAKLLIGLQVTRGITKEI
                     SRRIGGSDGCVHLRELALETINFAATVMVGYDEGFGLMSREFNILDEHKRYDISKSIL
                     RNTCHIYKQPRTQEGETV"
     gene            complement(2347..>3401)
                     /locus_tag="PK754_14305"
     CDS             complement(2347..>3401)
                     /locus_tag="PK754_14305"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNB10723.1"
                     /translation="NMGVYKDIRFKDERIPDIHMVFRYDRNGKDVKKQVMADVANSYM
                     FFQEKFGSLPIGKLVAAEYSYSHSEAYDGLVNISASVFNSNDDDGVDEWIRAHEVAHQ
                     WWGIGVDFDSYHDQWLSEAFAEYSGMLYVQQSLSDNKEFFRFLNITKERIFKNRKGIF
                     VDGQEAGPISLGYRTSSSSTRGDYGLVIYSKGMWVLHMLRNMMLDLNTMNEDRFFAMM
                     KDYFETYYGKKATTDDFKRIVEKHVGTDMAWFFDQWIHGTDLPKYEFAYTTKQTENGK
                     FKVRCRIRQENAGENFQMFIPITIDFGDNRKARMRLLVKGPQSEIDLPLLPLEPKKII
                     FNDFESVLCEVNYSNW"
BASE COUNT          922 a          777 c          666 g         1036 t
ORIGIN      
        1 ctgtttggta tcatcgtgat ctttgagatc tttgaccaga ttcaaggccg cattgtaata
       61 ctcaaacgat ttgcgaaaat ctttggcgac aaaagcctca cggcctttat ccatgtgttg
      121 tttgtactcc tcattcatgg tatcgaactc cctgattcac ataaaaaaac atatagatta
      181 aaaattgagt gttaatgtac aagaaaagga aggtcgcatg caagaacgta tttttagtgt
      241 ttcaaaaacc gttcggccag tgctagatct tccggcgttg tgatttttat attatcatag
      301 tgaccttcaa cgatttttac ggcatgacct atgcgttcga tcagcatagc ttcatcggtt
      361 ccatagtagt tttcagcata cgctttatcg aaagcgctcc gtaataacga atattgaaat
      421 gcttggggtg tctgtacatt ccacagagta ttgcgatcaa tagtacgcgc aacaaaacca
      481 tttgcgtctt gttgtttgat cgtatctttt ggtcttacag ccagtatggc ggccgaatat
      541 tcgacggtta tttcgataag ctcatctatt ttatcggcac gtacacacgg acgcacggca
      601 tcgtgaacaa gcacaatatc tgactcctcg accgctttta ggccttcgta aacactgtct
      661 tgtcgttctg tgccgccggg cactaatgta tgaatacgac taaagccgta cgggccgatg
      721 atttcttctt gaacaaacgg tatccactca ttttgcagta ctaatatgac ttcatccaca
      781 tagatcgcat gatgaaattt ttcgagggta tgtacaagaa taggcttccc gtggacttcc
      841 aaaaattgct ttttggaacc gacgcccatg cgtttaccga tgccggccgc cggtaatatg
      901 gctgtaactt tcttcccttt gatcatatca tcctttaacc gcaactatat cgcccattcg
      961 aaatcctttt tcgtcgcggc gtacaccgga catagcggtc tccggatcat gctcgaaaaa
     1021 taatatccac cggttttcga atgcgcgagt caatatgtcc tttttttccg ctatggttac
     1081 caaaggttga ttatcatacg ccatcacgta gggtacggca atatgtgtcg tcgtcggaat
     1141 caaatcaccg caaaaaaaga ccgtatgctg cgcatcatgg ataataggaa gttgttgata
     1201 cggcgaatgc ccattggcta taaaaaaagt aatattgggg aaaatgccgt cggtttgttc
     1261 gatcaattgc aactgacctg attggacaag cggctcgtaa tccggtttca gaaagctggc
     1321 acgatctttt tcagatgctt tttcacccca ttcgtaatgt ttttgttgaa cataatattt
     1381 ggcgttggga aaagcggcga cgatccggct ttgttcatcc attttggtcg aaccgcccgc
     1441 atggtcaaaa tgtaagtgcg tcagaatgac atccgtaata tcgtttgggg tgagatcgta
     1501 tttttgcaaa gatcgctcca gtgtgtaaac cgaatgatca atgcgataca tggaggcaaa
     1561 tttttcatta tatttatgac cgacaccgtt atctatcagt attttttttc catcatccga
     1621 aacgagcaat aaacaacgca ttgccagcgt aatgcgattt tgttcatcgg ccggattggt
     1681 tttattccac agatttttag gcactacacc aaacatcgca ccgccgtcta gtgcaaaacg
     1741 acccgtttcg accggatgta aatggtacgc tcctattttg gtatccacct tcataccgtt
     1801 tctccttctt gcgtccgagg ttgtttataa atatgacagg tatttcgtaa aatactttta
     1861 gagatatcgt agcgcttatg ttcgtccagt atattgaatt cgcggctcat aagcccaaat
     1921 ccttcatcat agcccaccat aactgtcgct gcaaaattta tcgtttccag cgccagttcg
     1981 cgcaaatgta cacaaccgtc gcttccgccg atgcgccggc taatctcttt ggtaatgcct
     2041 cgtgtgactt gcagaccgat caaaagtttg gctttctctg ttaccccggg gcatgctgaa
     2101 tgcggatgat tagccatttc catcttggaa ctcacgatat accgagtgtg cggatcaatc
     2161 tgaatattga gcctcaataa atgatacgga tctaaaaaag tcgcttcaag catgacgttc
     2221 ccgttttcca attcaaacgc ggatataccg atatctctct tggatactaa gcgttttacc
     2281 gggcttgggt ctgccggttt gaataccggc gctatcgttt cactcataat tattaatcct
     2341 attacattac cagttggaat aatttacttc acacaatacg gattcaaaat cattaaagat
     2401 gatttttttg ggttccaaag gcaacagagg tagatctatt tcagattgcg gaccctttac
     2461 caataaacgc atccgtgctt tccggttatc gccaaaatca atagttatgg gaataaacat
     2521 ttgaaaattt tcacctgcat tttcctgacg aatacgacag cgtactttga atttcccgtt
     2581 ctctgtttgt tttgtagtgt atgcaaattc atattttggt aaatctgtcc catggatcca
     2641 ttgatcaaaa aaccatgcca tgtccgtccc aacatgcttt tcgacaattc ttttaaagtc
     2701 gtcggtagtg gctttttttc cgtaatacgt ctcaaaataa tctttcatca tcgcaaaaaa
     2761 acgatcctcg ttcatcgtat tgagatcaag catcatgttg cgtagcatat gcaataccca
     2821 catacctttg ctatagatga ctaaaccata atcgccgcgc gtagaacttg aagacgtccg
     2881 gtatccgagc gatatgggac cggcctcttg tccgtcaacg aatataccct tacggttttt
     2941 aaaaatacgt tccttggtga tatttaaaaa ccgaaaaaat tctttattgt ccgataaact
     3001 ttgttgtacg tacaacatac cgctgtattc ggcgaaggcc tcgctcaacc attgatcgtg
     3061 gtacgaatcg aaatcaacgc caatacccca ccactgatgc gctacttcgt gcgcacgaat
     3121 ccactcgtca acgccgtcat cgtcattgga gttgaaaact gatgctgaaa tattgaccaa
     3181 cccgtcatag gcttcgctat gtgaataaga atattctgcg gctacaagtt tacctatagg
     3241 taatgaacca aatttctctt gaaaaaacat gtaactattg gctacatccg ccatgacttg
     3301 ttttttaaca tctttaccgt tgcggtcata acgaaacacc atatgaatat caggaatgcg
     3361 ttcgtcttta aaacgaatat ccttgtaaac gcccatatta a
//
LOCUS       DAOQNQ010000329         3395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_13718_length_3395_cov_7.172754, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000329 DAOQNQ010000000
VERSION     DAOQNQ010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13718_length_3395_cov_7.172754"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PK754_14310"
     CDS             <1..498
                     /locus_tag="PK754_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10724.1"
                     /translation="DRIDSMVSSASQFTRILGDALTGQAKNFRQRILQAARDFAAELI
                     SSMALSGISKLFGGGGSFGLFGTLFGGFRANGGDVQAGKSYIVGERGAEMFTPKTNGY
                     ISPNKISSTGFDPIASSVPSKQSQQIEVMNNLQLFIDSQQVDASIKRNGSRLNTRRGL
                     TGATA"
     gene            561..>3395
                     /locus_tag="PK754_14315"
     CDS             561..>3395
                     /locus_tag="PK754_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10725.1"
                     /translation="MDDMTFPRTYYSGAVDNAAGYAAGVSTLVADGNFVGEDPTGYII
                     YFAGHNAHYRIESTSIAGVLKLNLNLDKPLEKAIVDNEVITICRWYDITDCAVDVQDL
                     EQEIEVNQNELVLEKQIRFRLFNFDNTFYDRTAFTSAWDNVAAYFTTRNTQNAGDNKY
                     VGIWVYNQTTAAYQQFYAGQIVRDEVQINEPQSEIRFMTESVFYGLNDITIKEVDNIF
                     NGHYYNSSYYSSDTTKTQTVSGRYIDIIVKMFHAVGVPKITHDVTSREKNSGQCKIGF
                     VTDPQIIAGEYIEVPTTMGNEYAGLHVCDGAETISGTYYIYYTFGEDTSALDNSDVSG
                     GLTSHRVYFDDYDDFWDLSHESVRYYFNELWFIVQKWRNNEINETQANEILKDICYTF
                     TAFATVRFIEGRVYGLFRAKDNSSPIKLTIGQDDFIGDAEIIGNWRYVSKDGVKINTT
                     ANYFDGTSQDESFNYNDELMGVITSVTDDSDNGDYYLEVMDDLNNLGSTDLSDATNRR
                     IAFSNHDSSYLYTADADHITLNQLNKLKSALHDESFVFKEPQQNKTIDYADCFNIKLK
                     KPLLYVTDFNNGKILKYTGVSYGSWTGSQVDTLTEVLGISALQFLPPDVSAGGVIKSN
                     SFGHGFFVTVYDTISASYLYSYGTIGFDRTNKGSIKSFSSGGDHVISSNFCYFKHPST
                     REYGHYLVISFADMDKIKLFIISQENQNNYYTDETLSSKIKGTFGIYKFTATEHTHYT
                     LAGPSQLFYSAKINGNVLSPYCDEYVYMAWQTITTFRIYKTPLSYSTSLDAGAIGTET
                     PTSGASLLDLHLDYIYYTLGALTGNDIIRKVQSGQNLSTAVDVVGGGATEPAAAGSSV
                     SGLSNVLFDGLRSFWISYNGDIYFANKGTTDYSVGVCDVVGDVVYTIDRSDTSAVLVE
                     ASRITGLDFIKTYKTIDGEDYFMSISYATSK"
BASE COUNT         1048 a          614 c          778 g          955 t
ORIGIN      
        1 gatagaatag attcaatggt aagttccgcc agccaattta caagaatatt gggcgacgcg
       61 ctaacgggtc aagcgaaaaa tttcagacaa cgtatattac aagcggcgcg ggactttgcc
      121 gcagagttaa tatccagcat ggcattgtcg ggcatatcta aactgtttgg cggtggcggt
      181 tcgtttggac ttttcggcac tttgtttggc ggctttcgcg caaacggtgg cgacgtgcag
      241 gcgggcaaat cttacatcgt tggggaacgc ggcgcggaaa tgtttacgcc taaaacaaac
      301 gggtatattt cgccaaacaa gataagctct acaggattcg acccaatagc gtcaagcgtc
      361 ccgtctaagc aatcgcaaca aattgaggtt atgaataact tacaactatt cattgatagc
      421 caacaagttg acgcttcgat caagcgtaac ggttcgcgct taaacacacg gcgcggacta
      481 acgggagcga cggcatgagt acgcaacaag tcgcaaaaat acgcatagca ccgtttaata
      541 ccgccgaata cggtgagtat atggatgaca tgacgttccc gcgcacgtat tatagcggcg
      601 cggtggacaa tgcggcgggg tatgcggctg gcgtgtcaac gcttgtggcg gatgggaact
      661 tcgttggcga agaccctacg ggctacatca tttattttgc cggacacaat gcacactatc
      721 gtatagaatc aacttcgatt gccggcgtat taaagttaaa tcttaacctt gataagccgc
      781 ttgaaaaagc tatcgttgac aacgaggtaa taacgatttg taggtggtat gatataaccg
      841 attgcgccgt tgacgttcaa gatttagagc aagaaataga ggtaaatcaa aacgagcttg
      901 ttttggaaaa gcaaataaga ttccgtttgt ttaattttga caatactttt tatgatagga
      961 ccgctttcac aagcgcatgg gataacgtag cggcatattt cactacgcgc aacacgcaaa
     1021 acgcgggtga caataagtac gtaggtatat gggtttacaa tcaaactacg gcggcatatc
     1081 agcaatttta cgccgggcaa attgtacgcg atgaggtgca aataaacgag cctcaatcag
     1141 agattcgttt catgacggaa agcgtatttt acggattgaa tgatattacg ataaaagaag
     1201 ttgataatat attcaacggg cattactaca atagcagcta ttattcatcg gatacaacta
     1261 aaacgcaaac ggtatcgggt aggtatattg atataatagt aaaaatgttc catgcggttg
     1321 gtgtacctaa gataacgcac gatgtaacaa gtcgtgaaaa aaatagcgga caatgtaaga
     1381 tcgggtttgt taccgacccg cagataatag cgggggaata tattgaagtc ccgactacga
     1441 tgggcaacga atacgcgggt cttcatgtat gcgatggggc ggaaacaata tctgggacct
     1501 attatattta ctatactttc ggcgaggata catcggcact tgataattca gatgtgagcg
     1561 gcgggttgac ctcacacagg gtttactttg atgactatga tgatttttgg gatttatctc
     1621 atgaaagcgt ccgatattat tttaatgagc tttggtttat agttcaaaaa tggcgcaata
     1681 atgaaataaa tgaaacgcag gctaatgaaa ttctaaaaga tatttgctat acatttaccg
     1741 cgttcgctac ggttcgtttt atagaagggc gcgtttacgg gctattccgc gcaaaagata
     1801 attcgtcgcc aatcaaattg acaataggtc aagatgattt tataggcgat gccgaaataa
     1861 taggtaattg gcggtatgta tcgaaagacg gagtaaaaat caacacaacc gcaaattatt
     1921 ttgacggcac atcacaagat gaatcattta attacaacga tgaattgatg ggcgtaatta
     1981 caagcgtcac agatgattcg gacaatggcg attattattt agaggtaatg gatgatttaa
     2041 acaatttagg ttctaccgac ttaagtgatg caaccaatag gcggattgca ttttctaatc
     2101 atgattcaag ttatttatac acggcggacg ccgatcatat caccttaaac cagttgaaca
     2161 agcttaagtc ggcgttgcat gatgaatcgt ttgtttttaa agagccgcag caaaataaaa
     2221 caatagacta tgccgattgc tttaatatca aactaaagaa acctttgctt tatgtgacag
     2281 attttaataa tggtaaaata ttaaaatata ctggcgtaag ttatggcagt tggacgggtt
     2341 cgcaagtaga cacgttaacc gaggtattag gtatatcggc tttacaattt ttgccgccag
     2401 acgttagcgc aggcggcgtt atcaaatcaa attcatttgg tcatggtttt tttgtcacag
     2461 tttacgacac tatatccgcc tcttatttgt attcatacgg cacgattgga tttgatagga
     2521 caaataaggg atcaataaag tcttttagta gcggcggtga ccacgtaata tcatctaatt
     2581 tttgctactt taagcatccg tcaacgaggg aatatgggca ttatttggtt atatcattcg
     2641 ctgatatgga taaaatcaag ctatttatta tatcccaaga aaatcaaaac aattattaca
     2701 cagatgaaac gttatcgtcg aaaataaaag gaacttttgg tatatataaa tttacagcga
     2761 ctgaacacac gcattatact ttagccggtc cgtctcaatt attttatagc gcaaaaataa
     2821 acggtaacgt gctttcccct tattgtgacg aatatgtata tatggcgtgg caaacaatta
     2881 caacatttag aatttataaa acgccgttat cgtattctac atcgcttgat gctggggcta
     2941 taggtacgga aacgcctacg tcgggcgcgt cacttttaga tttacattta gattatatat
     3001 attatacgct tggggcttta accggtaatg atataataag gaaagttcaa agtggccaaa
     3061 atttatcaac ggctgtggat gttgttggtg gtggcgcgac agaacccgcc gccgccggct
     3121 catccgtttc gggtttgtct aatgtcctat ttgatggatt gagaagtttt tggatttctt
     3181 acaatggcga tatttatttc gcaaataaag gtacgactga ttattcagtt ggcgtttgtg
     3241 atgttgtggg cgatgtagtc tatacgatag acagatcaga tacatcggcg gtattagttg
     3301 aggcgtcaag aataacaggg cttgacttta taaaaacata taaaacgata gacggcgaag
     3361 attattttat gtcaatatct tatgcgacgt caaaa
//
LOCUS       DAOQNQ010000330         3393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_13730_length_3393_cov_6.082085, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000330 DAOQNQ010000000
VERSION     DAOQNQ010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13730_length_3393_cov_6.082085"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..1264
                     /locus_tag="PK754_14320"
     CDS             191..1264
                     /locus_tag="PK754_14320"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009556901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNB10726.1"
                     /translation="MYQSIIRPILFRFDPEVVHETISRGLFASEPLLRHINFLFCNNS
                     SVLSQTLHDINFTNPIGLAAGFDKYGMGVPVWPHLGFGFAEIGTITGQAQDGNPRPRL
                     FRLPENKALINRFGFNNHGADQTAQTLAQWKRENTLHRIPLGINIGKTKIVSLDHAVE
                     DYAYTFSRLFDYGDYFVVNVSSPNTPNLRKLQDKAFLDAIFSSLMKINREQSGSKLLK
                     PLFVKIAPDLTLSQIDDVLSSIENHKLHGIIATNTTITRENINSRYAGETGGLSGFPL
                     RGMSTEIIRYIHKVTSGKLTIIGAGGVFTAEDAYEKIKAGASLIQIYTGFIYEGPRFC
                     YNLNAGLLELMKRDRIKSIRDLR"
     gene            complement(1294..1977)
                     /locus_tag="PK754_14325"
     CDS             complement(1294..1977)
                     /locus_tag="PK754_14325"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNB10727.1"
                     /translation="MRFNLILLSTITVFCFAACQPIGDSVSADAPVIDGIMAARNTIL
                     VNDTTNITVSARDLNDEQLDYVWTISTNNGGSFEGAINGSTVTYRAPFLPSSYTIKCK
                     VVNESNKSASKDIKITVTNIGTPIVEITQPTDAAFISASAGIYSIKANVKNADASSVD
                     SMVCYIDNKRLSRKTLTNNTTFDWDVTALNGSKRITVQAWARISGPATTDSSVVNVSI
                     EGTISKPGR"
     gene            complement(1986..2777)
                     /locus_tag="PK754_14330"
     CDS             complement(1986..2777)
                     /locus_tag="PK754_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10728.1"
                     /translation="MKFIQTYFFILVIIAGNAGCDLKHDNTLDPKNPNAAADQIAVVE
                     NFVIINTNVNVTAPEFNTYSLNALYELEERYDNNLIALEYHMTPNSGGFEDSLAISDN
                     ELRYANEYKGSASRGFPHTFFNGKSVAVQGASSLSVAKQRYQTVLDSVVSNKEKLYVT
                     ATKKIDASQLTLEIQIARYGDEDLTNAKIEYIVYEDKGMPSGHYTVREVRPSETLPLI
                     PAKTIYEFTTDPINIKSSYVQSQIGVVVIIKSALNGRILQAARAF"
     gene            complement(2783..>3393)
                     /locus_tag="PK754_14335"
     CDS             complement(2783..>3393)
                     /locus_tag="PK754_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10729.1"
                     /translation="DLSEDKDKQAKVGNLIGADYVITGEVSEAEGRLRIDVAVTRISS
                     GQVIAEKVTGPSRKYVNTMARLLANNIAYELSGEGKKIPSMKLGGAPVTEFAIATLVL
                     GGGAYLAAQKTSDLRAAYRGTTDLAKMNARYKKADRWNKVTISLQVGTAATAIGLVYA
                     FIKNASSDREVLADMDLAEKSNFAVAPYIDSKTSGLNLSFSF"
BASE COUNT          959 a          736 c          687 g         1011 t
ORIGIN      
        1 cgtaccgatt ataattttga gagtgttgcg aagagccagt tttaatgtca tatcaaaaat
       61 tattaatgaa aaccgtggcg gaaactacaa aaaatacttc tcactttcaa acgatttacc
      121 tacaaaattg ttttgtcttt tatccgtgta tgtatatttt ttaacaccat gatgtaaatt
      181 cggagaacgc atgtatcaat ccattatcag gcctattctt tttcgatttg accctgaagt
      241 tgtgcacgaa accatatcgc gcgggctatt tgcaagcgag ccgttactta gacatataaa
      301 tttcttattc tgtaataatt cctctgtcct ttcgcagacc ctacatgata ttaattttac
      361 aaaccctata ggtctggcgg ccggttttga taagtatggc atgggcgttc cggtttggcc
      421 gcatttgggt ttcggttttg cagagatcgg tacgataacc gggcaagcac aagatggcaa
      481 cccgcggcca cgtttattca ggttaccgga aaacaaggca ctaatcaatc gctttggctt
      541 taataatcat ggcgctgatc agaccgcaca gactcttgcg cagtggaaac gtgaaaatac
      601 gttgcaccga atacctctcg gcatcaatat cggtaaaaca aaaattgttt cgcttgatca
      661 cgctgtcgaa gattacgctt atacgttttc ccgtttgttt gattacggcg attattttgt
      721 cgtcaatgtg agctcaccca atacgcccaa tctgcgcaaa cttcaggaca aagcttttct
      781 cgatgcgata ttttcgtccc tcatgaagat aaaccgtgag caaagcggta gcaaactgct
      841 taaaccgctg tttgtcaaaa tagcgccgga ccttacactt tcacagattg acgatgtgtt
      901 aagttcaatt gaaaaccaca aattacatgg tatcattgca accaatacga cgataacgcg
      961 tgaaaatata aattcgagat atgcaggtga aaccggagga ttgagcggat ttccactccg
     1021 tggtatgtca acggagatta tacgttacat tcacaaagtt acatcgggaa agctgacgat
     1081 tatcggtgcc ggaggtgttt ttacagctga agatgcgtat gaaaaaataa aagccggagc
     1141 ttcgttaata caaatataca ccggatttat ctatgagggg ccacgttttt gttataatct
     1201 aaatgctggt ttattagaac ttatgaaacg tgatagaatc aaatcaatac gagatttacg
     1261 ttaaaaaact ttttttatat aatgccctaa tgctcatcgc cccggcttag atatcgttcc
     1321 ttcaatagat acgttcacta cagacgaatc cgtagttgca gggccactaa tacgcgccca
     1381 tgcttgcacg gtaatccttt tagatccgtt caaagctgta acatcccaat caaatgtggt
     1441 attgttagtc aacgtcttgc gggaaagtcg tttgttgtca atataacaaa ccatgctgtc
     1501 cactgaggat gcatctgcat ttttaacatt ggcttttatt gaataaatac ctgcgctagc
     1561 agagataaat gcagcatccg taggttgcgt gatttctact atgggtgtac cgatattagt
     1621 gactgtaatc ttgatatctt tactggcact tttattagac tcattaacga ctttacactt
     1681 gattgtgtaa ctgctgggta aaaaaggcgc tcggtaagtt actgttgatc cattaatagc
     1741 gccctcaaat gatcccccat tgttggtact aatggtccaa acatagtcca actgttcatc
     1801 gttcaaatcg cgtgctgaaa cggttatgtt tgtcgtatca tttacaagta tggtatttct
     1861 tgctgccata ataccgtcaa taaccggagc atcggcacta acgctgtccc ctatgggttg
     1921 gcatgcagca aaacaaaaaa cagtgattgt tgataaaagt attaaattaa agcgcatttt
     1981 tcctcctaaa acgcccgcgc ggcttgaaga atacggccgt ttaacgcaga cttaataata
     2041 acaaccacac cgatttgcga ttgcacatag ctggacttga tattgatcgg atcagtcgta
     2101 aattcataaa ttgtcttagc cggtatgagt ggcagcgtct ccgatggccg cacttcgcga
     2161 acggtataat gcccagatgg catgcctttg tcctcataga cgatatactc gattttggca
     2221 ttggtaagat cttcatcgcc gtagcgtgcg atctgtattt ctaacgtcaa ttgggatgcg
     2281 tctatttttt ttgtcgccgt gacatacagc ttttctttat tggaaactac agaatctaaa
     2341 accgtttggt accgttgttt ggcaacactt aatgacgaag cgccctgcac agcaacagat
     2401 tttccattaa aaaatgtatg tgggaatccg cgcgaagccg accctttata ttcattggcg
     2461 tatcgcaatt cattatccga tatggctagc gaatcctcaa atccacctga attgggagtc
     2521 atgtgatact ccagtgcaat caaattatta tcgtagcgtt cttccaattc gtataatgca
     2581 tttagagaat acgtattgaa ttcaggagcc gtgacattca cattggtatt gataatgaca
     2641 aaattttcga ctacggctat ttgatccgcg gccgcattgg gatttttagg gtctagcgtg
     2701 ttgtcatgtt ttaaatcaca accggcgttg ccggcaatga tcactaaaat aaaaaaatat
     2761 gtttgaatga atttcatagt tttcaaaatg agaatgaaag gtttagccct gacgttttag
     2821 aatcaatata cggtgcgaca gcaaaattac ttttttcagc taaatccata tccgccaaaa
     2881 cttcacgatc ggaagaagcg ttttttataa atgcataaac taaaccgatc gcagtggctg
     2941 cagtgccgac ctgaagcgaa atcgtcactt tattccagcg atctgctttt ttatagcgtg
     3001 cattcatttt tgcaagatcg gtagtaccgc gatacgccgc acgcaaatca gaagtttttt
     3061 gcgctgctaa ataggctcca ccgccaagga ctaacgtggc aatggcgaat tctgtgaccg
     3121 gagctccacc caacttcata gacggtattt ttttgccttc gccggataat tcgtaagcta
     3181 tattattagc taataaccgc gccatcgtat tcacatactt acgcgagggt cctgtgacct
     3241 tctctgcgat aacctgaccg ctggaaatac gcgtaacggc tacgtcaata cgtaagcgac
     3301 cttccgcctc agaaacttca ccggtgatca catagtccgc tccgatcaaa ttcccgacct
     3361 tcgcttgttt atccttatct tcggacagat cac
//
LOCUS       DAOQNQ010000331         3393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_13734_length_3393_cov_3.973337, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000331 DAOQNQ010000000
VERSION     DAOQNQ010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13734_length_3393_cov_3.973337"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..1528
                     /gene="glmM"
                     /locus_tag="PK754_14340"
     CDS             197..1528
                     /gene="glmM"
                     /locus_tag="PK754_14340"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNB10730.1"
                     /translation="MVSVSGIRGIIGESLTPEVITKYAAAFGTWCNGGTIVVGRDSRV
                     SGEMVKTLVTGTLLAAGCKIVDIGIVPTPTVEITVNDMNAHGGIAITASHNPIQWNAL
                     KLIGPRGRFLTQSQMNEVLEIADRKSTLLVTWDRIGKINYYDHAIQNHIDHILELEYI
                     DVEALRKRNFKVVVDCVNGAGGVIVPRLLKELGCNVVVINEEPHGVFPRNPEPSVDNL
                     KALEETVRQHHAHIGFAVDPDVDRLALVNEKGQAIGEELTLMIATDLVLSRKKGPVVV
                     NASTTMGIDQIASMYGVQTHHTRIGEINVSTKMEEIDSVIGGEGNGGVILPEIHLGRD
                     ACTGIALALQYVLQKNKPFSECVAELPYFIMKKDKIELHDIDADAVMTKILKDQKKGR
                     VDTVDGVKIIFEDGWIHFRKSNTEPIMRIIAEARTQERLDNMIQSFTSYFH"
     gene            1570..2748
                     /locus_tag="PK754_14345"
     CDS             1570..2748
                     /locus_tag="PK754_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10731.1"
                     /translation="MKRILFFLMLGTLLIGSSSVFAQKPTWVDNPGSYSAENFVSVGV
                     AKDSRPDKAKEKAEKKSKAALEKLLRNKYPKKDVKGAMTSLHFEAYYQDPATKYYYVL
                     ALIPIETLDKDYAKQKMMDRNRSAAMGAVAMLNAQTKKDPDVIIVRVDEEVGEEPAME
                     AATPEESEKPVVSPKTTTPVSTTPSTNATSVNSTVNSAPNGGSGVTDFKSKSLGNYKW
                     TDQDQNSKYSFLGDGHMTVSIASGEYWNPQEPNKSAPRLELPNVKGDFEAIVKVKMDW
                     SRYYSAGFGLTAHNGNQNVRAFVFYNGAYVYVEGYAGGIEVPRTEKSIEAFKDFGYLK
                     LVRKGYTWTAYYSTIADDWVEYATFETEFPETANVGLVLLNAEGSTVQAKVEFVQITQ
                     "
     gene            3125..3197
                     /locus_tag="PK754_14350"
     tRNA            3125..3197
                     /locus_tag="PK754_14350"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3158..3160,aa:Gly,seq:tcc)
     gene            3219..3295
                     /locus_tag="PK754_14355"
     tRNA            3219..3295
                     /locus_tag="PK754_14355"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3253..3255,aa:Thr,seq:ggt)
BASE COUNT         1030 a          703 c          785 g          875 t
ORIGIN      
        1 aacaaaacca agaccgacca aagaccttcc tctaaagaag aaacataccg gtgtccccgg
       61 tggagatgga aaaactgttc aaattcaatc taaaggccga ctaacaaaga ttctgtccga
      121 aaaatacaga aacgaaaaaa agtcactacc ggccaaaccg tcttcacact ccgcaccaaa
      181 agataccgga acgctgatgg ttagcgtttc cggcatacgt ggtatcatcg gtgaaagtct
      241 tacgccggaa gtgatcacaa aatacgcggc cgcttttggc acgtggtgta atggcggtac
      301 gattgttgtt ggtcgggatt cgcgtgtgtc cggcgaaatg gtcaaaacac tcgtgaccgg
      361 tacattgctt gccgcgggat gtaaaatcgt agacatcggt atagtgccta cgccgacggt
      421 agagatcaca gtcaacgata tgaacgcgca cggcggtata gctatcaccg caagccataa
      481 tcccatccaa tggaatgcgc tgaaacttat cgggcctagg gggcgttttt tgacacagag
      541 tcagatgaat gaggtactcg aaatagcgga tcgtaaatcc accttgcttg tgacatggga
      601 tcgcatcgga aaaatcaact attatgatca cgccattcaa aatcatattg atcatattct
      661 agaactagaa tacatcgatg tggaggcgct gcgaaaacgt aacttcaaag ttgtagtgga
      721 ctgcgtgaat ggcgcaggcg gagtgatcgt acccagactt ctcaaagaac tggggtgtaa
      781 cgtagtggtg atcaatgaag aaccgcatgg cgtgtttcct cgtaatccgg agccatctgt
      841 tgataatcta aaagcactcg aagaaacggt tcgccaacat catgcccata ttggttttgc
      901 cgttgatcct gatgtcgata ggttagcatt ggtcaacgaa aaaggccaag ccatcggtga
      961 agaattgacc ttaatgatcg caacggacct cgttttatca cgcaaaaagg gtccggttgt
     1021 cgtcaatgca tccaccacga tgggcataga tcaaattgcc tccatgtatg gcgttcaaac
     1081 ccatcatacg cgcattggtg aaatcaatgt ttctaccaaa atggaagaga tagattccgt
     1141 tatcggcggc gaaggcaacg ggggcgtgat cttaccggaa attcatcttg gccgtgatgc
     1201 atgtaccggg attgctcttg cccttcagta tgtgttacaa aaaaataaac cgttttcaga
     1261 atgtgtagcc gagcttcctt attttatcat gaaaaaagat aaaatcgaat tgcatgatat
     1321 tgacgccgat gctgtcatga ccaaaatact taaggatcag aaaaaaggac gtgttgatac
     1381 ggtggatggt gttaaaatta ttttcgaaga cggatggatt cattttcgca agtccaatac
     1441 cgaaccgatc atgcgtataa tagccgaagc gcgtacgcaa gaaagattgg ataatatgat
     1501 acaatcgttt acctcatact ttcattaagt atgtatatcc gttcagaact atactcagag
     1561 gagacataca tgaaaagaat tttgtttttt ttgatgctag ggacgttgct gattgggtct
     1621 tcgtccgtat ttgcacaaaa accgacttgg gtggataacc ccggttccta ttcggccgaa
     1681 aattttgttt ccgtaggtgt cgctaaagac agccggcctg ataaagccaa agaaaaagcc
     1741 gaaaaaaaat ctaaagcagc actcgaaaaa ctgcttcgaa acaagtatcc caaaaaagac
     1801 gtcaaaggcg caatgacgtc gttacatttt gaagcgtatt atcaggatcc ggcaacaaaa
     1861 tattattatg ttttggctct tattcctata gaaacgttgg ataaagatta tgccaaacag
     1921 aaaatgatgg atcgcaatcg ttctgctgcg atgggtgccg tagcaatgct caacgcgcaa
     1981 acgaaaaaag atccggatgt aatcatagtg cgtgtagatg aagaggtagg cgaagaaccg
     2041 gccatggaag cggctacgcc agaagagagc gaaaaacctg ttgtatcgcc taagacgaca
     2101 acacccgttt ctacaacgcc ctccacaaat gcgacttcgg ttaattcgac ggttaacagc
     2161 gcgcccaatg gtgggagcgg ggttacagat tttaagtcca aatcacttgg gaactacaaa
     2221 tggacggatc aagatcaaaa ctccaaatac agttttctag gcgacggcca tatgacggtg
     2281 tctatcgctt ccggcgaata ttggaatccg caagaaccga ataagagcgc cccgcgcctt
     2341 gagttaccca acgtaaaagg ggattttgaa gccatagtaa aagtaaaaat ggattggtca
     2401 cgctactata gtgccgggtt tgggcttacg gctcacaatg gcaatcaaaa cgtacgtgcg
     2461 tttgtgttct ataacggcgc gtatgtgtat gtagaaggtt atgccggcgg catcgaagta
     2521 ccgcgcacag aaaaaagtat tgaagcattt aaggatttcg gttatctcaa acttgtgcgt
     2581 aaaggctata cttggacggc ttattacagc acgatcgccg acgactgggt agaatacgct
     2641 acatttgaaa cggaatttcc tgaaactgct aatgttggct tagtattgct taacgccgaa
     2701 ggttccacgg tacaggccaa agtggaattt gttcagataa ctcagtaatt cgcgatattt
     2761 tgcctaaata aaaggttgcc ttaagagctc ttttttgaaa acggacagat gtggtatttg
     2821 ttttaatctt gaaaaaaata aatttcacat tctatacgtt cgaattaagc ttttgaggca
     2881 atctttctat tttttcaggt cttgacacat ttttcatttc ctaccgtttt aaacatccat
     2941 taaaagcaac aaaaacaaga aaatgcttga catgcgaggg ctttttgtat atatttgcac
     3001 ctcgtttgga taactatata tttttattag cttttcgctc ttaagtgtag catgtgttga
     3061 acttagaaaa ggctacttgt ccaacgaaaa atgctcttta aaaaaaaata ttttgtatgt
     3121 aattgcggga gtaactcagt tggtagagtt cttgccttcc aagcaagatg tcgcgagttc
     3181 gagtctcgtc tcccgctcga taaatgcagt attgcaaagc ctgtgtagct cagatggtta
     3241 gagcgcgtcc ttggtaagga cgaggtcatc ggttcaattc cgatctcagg ctccacgaac
     3301 aacatcaact tactcttgac actcaatacg aggagatgcc atggcaaaag agaaatttaa
     3361 ccgttcaaag ccgcacgtga atgtgggcac gat
//
LOCUS       DAOQNQ010000332         3380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_13815_length_3380_cov_5.766316, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000332 DAOQNQ010000000
VERSION     DAOQNQ010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13815_length_3380_cov_5.766316"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1435
                     /locus_tag="PK754_14360"
     CDS             <1..1435
                     /locus_tag="PK754_14360"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HNB10732.1"
                     /translation="FGILYRRNTITKTMVFTALLALLIAFGKFFEPFFKLMLSILPFF
                     EKFRVPSMILTLFIFAVTVLSCYGLDYIFNPTEEERKRRGGLEKVLLYVMGGIAVLGV
                     VSLLMPDFWSGMAGFLADGDAQRYNANQIAQLKKLRLDALNSGVIRFAVLGVIIFGAL
                     YLYMKEKIGAGLALMIMLMVNITDTISLNMKTLRPVSRAATQQEFQETETIRFLHSDT
                     TRFRIFSLLEHAQSGSPVWNYFGIESLSGYSPAKMRIYQDVIEFSMYKGPDPQFPINM
                     NVVNMLNAKYVLANGRLPEGMGFLMRKIDEAGKTLIYENKNVLPRAYFVSSVETIGER
                     QQALLRLNSVDFDVHRSAFTDKAIKTPITPFDSAKAEITQWTTDKISISAFVDKPGFM
                     VLSETYYPHGWKAYVDGQETEIYKTNYILRGIQVPAGQHTIEFRFEPAEYTAGIWAST
                     ISFFGVIGLLVVGGVMQYRRLQNEPKS"
     gene            1467..2510
                     /locus_tag="PK754_14365"
     CDS             1467..2510
                     /locus_tag="PK754_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10733.1"
                     /translation="MKMFWFFLFSVLTVSSSQAQEEYMTQTLNLKVYVKLINDVQPLL
                     DKTIEETGSRILSSTQGSLQYNTTANSLRMTIWSSSTGFVKLQQTIKSLGLVRHHEIT
                     TVNNADILASLEMELDHLNKKKQSYESEISRIDASSEKHGFLWDELRTIEDRIFETER
                     SILEKKQQTSNYVIELEVIEESYAPFEDAQDVQFVNMPGFEYAELRIENPLNTVSHKT
                     YRGGMVKYLFTKGKSYVRFGVLRSGQDGATPDEIEELFFYSFGQDFYPRYFGRGARSF
                     ANLYTGYSTGGVFATADDRRKNVFFLMPDIGLELYKTRFILWDTQAGYFIPFYQNRNF
                     RGIAGHMSFNFVF"
     gene            2518..>3380
                     /locus_tag="PK754_14370"
     CDS             2518..>3380
                     /locus_tag="PK754_14370"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNB10734.1"
                     /translation="MKIWVRRTLFGLTALLLVLTGLVLFGYFAARKSLPQTEGQLSLR
                     GLSAPVTVYRDEFAVPHIDASGETDLMRATGYVQAQDRLWQMDLLRRIAEGRLSERFG
                     TKTLSVDKALRTIGFMRMATMLKDSLPPDVMALLQAYADGVNAFIEENKGNYPPEFTL
                     IDFTPEAWKPEHSIALSRLLGWQLSMGWHVDVTYDRILDTVGFAKLQEILPRFPADAP
                     TIVPMVPPDYNAKSVLNGISENTYHEEHFRPAPKAAAAELRHFADALYEVKRIIGIEG
                     FSVGSNNWVVSG"
BASE COUNT          872 a          677 c          846 g          985 t
ORIGIN      
        1 ttttggaatt ttatatcggc gcaatacgat cactaaaacg atggtattta cggcgttgct
       61 ggcattgctt atcgcattcg ggaaattctt tgagccgttt ttcaaactca tgctttcgat
      121 tttgccgttt ttcgaaaagt tccgtgttcc atccatgatt ctcacgcttt ttatttttgc
      181 cgtcactgta ctgtcgtgct acggtttgga ttatattttt aatccgacgg aagaggaacg
      241 caaacgcaga ggcggtttag aaaaagtttt actctatgtc atgggcggca tagctgtact
      301 gggtgtagtt tcattattga tgcccgattt ctggtccggt atggccggtt ttttggccga
      361 cggcgatgcg cagcgatata atgccaatca gatcgcacaa ttaaaaaaat tgcgtttgga
      421 tgcactcaat agcggtgtaa ttcgatttgc cgtgctgggt gtgatcatat tcggtgcgtt
      481 gtatctttat atgaaggaaa aaatcggggc tggcctagca ttgatgataa tgcttatggt
      541 gaatatcacc gatacgatca gtctcaacat gaaaacgctc cgtccggttt cgcgtgcagc
      601 tacgcagcag gagtttcagg agacggaaac tattcgtttt cttcactcgg atacgacgcg
      661 gtttcgtatt ttttctttgc ttgaacacgc ccaatcgggg tcgccggtgt ggaattattt
      721 tggcattgag agtctgagcg ggtatagccc tgctaaaatg cgtatctatc aggacgtcat
      781 cgaattttcg atgtacaaag gtccggaccc gcaatttccg attaatatga atgtagtcaa
      841 catgcttaac gctaaatacg ttttggccaa cggacgtttg ccggaaggta tgggttttct
      901 gatgcgtaaa atagatgaag cggggaaaac gctcatttat gaaaataaaa acgttttacc
      961 gcgggcctat tttgtttcat ccgtagaaac gatcggcgag cgccagcaag ccttactgcg
     1021 ccttaacagt gttgactttg atgtgcatcg ctcggcattt accgataaag cgatcaaaac
     1081 gccgataacg ccgtttgatt cggctaaagc ggagataaca caatggacga cggacaaaat
     1141 ttcgatctca gcgtttgtag ataaaccggg atttatggtg ctgagcgaaa catattatcc
     1201 gcatggctgg aaagcttatg tggacgggca ggagacggag atttataaaa caaattatat
     1261 tctgcgcggc attcaggtac cggcgggaca acatacgatc gaatttagat ttgaaccggc
     1321 tgaatatacg gccggcattt gggccagcac gatttcattt tttggtgtga tcggattatt
     1381 agtagttggc ggagttatgc aatatcgccg gctgcaaaac gagccgaaat cttgatctgg
     1441 gatttatttt aaaaagaggt gttatcatga aaatgttttg gtttttttta ttttctgtgc
     1501 tgaccgtaag ttcgtcgcaa gcacaagagg agtatatgac gcaaactttg aatctcaaag
     1561 tgtatgtcaa actgatcaac gatgtccagc ccttattaga caagaccatt gaggaaacgg
     1621 gaagtcgtat tttgtcttcc acgcaaggca gcttacaata taatacaaca gctaattcgc
     1681 ttcgaatgac catttggtcg tcgtcgacgg ggtttgtcaa attacaacaa acgatcaaat
     1741 cactgggtct tgttcgccat cacgagatta ccacggtcaa taatgcggat attctggcat
     1801 cgcttgaaat ggaattggat catctcaata aaaagaagca atcgtatgag agtgaaatca
     1861 gccgcataga tgcgtcgagt gaaaagcacg gttttttatg ggacgaattg cgtacgatcg
     1921 aagatcggat atttgaaaca gagcgatcta ttttagaaaa gaagcagcag acatccaatt
     1981 atgtgatcga actcgaggtc atcgaagaat cgtatgcccc gtttgaagat gctcaagatg
     2041 ttcagtttgt caatatgccc ggttttgagt acgccgagct gcgtatcgaa aatccgctaa
     2101 atacggtttc gcacaaaacg taccggggcg gcatggtcaa atatctcttt acaaaaggta
     2161 agagctacgt tcgttttggt gttttacgca gtggtcagga tggtgcaacg ccggacgaaa
     2221 tcgaggagct tttcttttac agttttggtc aagattttta tccgcgctat tttggccggg
     2281 gcgcacgttc gtttgccaat ctatatacgg gttatagcac gggcggtgtt tttgcgacag
     2341 ccgatgaccg tcgaaaaaat gttttttttc tgatgccgga tatcggtctg gaactgtata
     2401 aaacccgctt tattttgtgg gatactcaag ccggatattt tatcccgttt tatcaaaacc
     2461 gcaattttcg cggcatagca ggtcatatgt cgtttaactt tgtattttga gatcactatg
     2521 aaaatttggg ttcgccgtac gctatttggt ttaaccgcat tgttactggt tctgacaggg
     2581 cttgtacttt tcggttattt tgctgcgcgc aaaagtttac cgcagaccga ggggcagctt
     2641 tcgctcagag gattgtcggc accggtgact gtttaccgtg atgaatttgc cgtaccacat
     2701 attgatgcgt ccggcgaaac agacctgatg cgggcgacgg gttatgtgca agcgcaggat
     2761 cgtttgtggc aaatggattt gctccgtcgt atcgccgaag gacgcttgag cgaacgtttt
     2821 ggtacaaaaa ccttaagcgt agataaagcg ctgcgtacta taggttttat gcgtatggcg
     2881 accatgctca aagacagttt gccgcccgat gttatggcgc ttttacaggc ctatgctgat
     2941 ggcgtgaatg cttttataga agaaaacaag gggaactatc ctccggaatt tacactgatt
     3001 gattttaccc cggaggcgtg gaagcctgag cattcgatcg cgctgtcacg actacttggt
     3061 tggcagctga gcatgggatg gcatgtggat gtcacatacg atcgtatttt ggatacggtt
     3121 ggttttgcta aattgcagga gattcttccg cgttttccgg cagatgcacc gactatcgta
     3181 ccaatggttc cacctgatta caatgctaaa tccgttttga atggcatttc agaaaacaca
     3241 tatcatgaag aacattttcg ccccgctcca aaagccgcag cggcggaact gcgtcatttt
     3301 gccgatgcct tatatgaagt gaaaaggatc atcggcatcg aaggatttag tgtaggaagc
     3361 aataattggg ttgtatccgg
//
LOCUS       DAOQNQ010000333         3377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_13835_length_3377_cov_4.430765, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000333 DAOQNQ010000000
VERSION     DAOQNQ010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13835_length_3377_cov_4.430765"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2157
                     /locus_tag="PK754_14375"
     CDS             <1..2157
                     /locus_tag="PK754_14375"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNB10735.1"
                     /translation="INADTSVLDEKANAWIAAVAKDLTNNKSKSLIVAGRRQPAAVHA
                     LVLAINSALGNVGTTVQYHAAKDMMVSDRSGLAELTKSLSDGKVSTLVMLGGNPVYNA
                     PADLKFAEAMKKAEHTIHFNGYVDETGSNGVEWHIPASHFLEAWGDVRSANGTVSAIQ
                     PLIDPLYATHSDIEVLYLLTTGKEARGYDIVRETWKTLFAKGLDFEKAWRLALHDGVV
                     GDTATAPVTVKMDAKAASAFVAKNALPSGSADANNLEIVFYASSATFDGRFANNGWLQ
                     ELPDPVTKITWDNAAIMSHKTAEALKVKNILGEGKNHQPMIRLKYQGQTLELPVWVQP
                     GHADYSIAVALGYGRQSAGRVGNGVGADTYALRTSAAMDFDLGLTAEKLSKTYLIATV
                     QDHGGFDTDALADNEIQRRIPTIIREATIEEYKKDPNFITSGRTPGEKELYGKDGKPI
                     SIMNMHEYGPERFEKGEWNKKYQWGMSIDLNACTGCNACMIACQSENNIPVVGKEEVA
                     KGREMHWIRLDRYYIGKDVNEPEMQFQPIACQHCETAPCEQVCPVAATVHDSEGLNVM
                     VYNRCVGTRYCANNCPYKVRRFNFLEFHAGTSGFMESKPIESLKLGKNPDVTVRMRGV
                     MEKCTYCVQRISGARIAAKKEDRDIRDGEVVTACQQSCPTGAIVFGNINDPESKVSKM
                     KAMNRKYEVLAELNVRPRTSFLAKLKNPNPELTKNA"
     gene            2315..>3377
                     /gene="nrfD"
                     /locus_tag="PK754_14380"
     CDS             2315..>3377
                     /gene="nrfD"
                     /locus_tag="PK754_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNB10736.1"
                     /translation="MASSGFLLLLIAITYLLFEGTGIWGITNPVGWGLAIVNFVFWVG
                     IGHAGTLISAILFLFRQKWRTSINRFAEAMTIFAVICALLFPGIHVGRMWATYWFAPV
                     PNQMGMWPNFRSPLLWDFFAVGTYFTVSLIFWYVGLIPDLASLRDRAKTRVRQVVLGF
                     LSLGWRGGNKQWKHYEMAYLILAGISTPLVLSVHSIVSTDFATSIIPGWHTTIFPPYF
                     VAGAIFSGFAMVMTLAIVARKAYKLENIITLYHLENMNKIMLLTGMMVGYAYSCEFFI
                     AWYSGNMYERFAFINRAFGPYWWSYFSMIFCNVVVPQIFWSKKLRRNITVMFIASIFI
                     NIGMWFERFVIIVTLSRDYL"
BASE COUNT          834 a          797 c          878 g          868 t
ORIGIN      
        1 atcaatgccg acacttccgt attggatgaa aaagccaatg cgtggattgc tgcggtcgct
       61 aaagatctga caaataacaa atcgaagtcg cttattgtcg caggccgtcg tcagccggct
      121 gccgtgcatg ccttggttct cgcgatcaac agtgcactgg gcaatgtcgg aacgaccgtg
      181 cagtatcatg ctgcaaaaga tatgatggtg tcagaccgtt ccggtttagc cgaactgacc
      241 aaatccttga gcgatggcaa agtatccacc ctcgtcatgt taggcggtaa tccggtgtac
      301 aatgcacctg ccgatctcaa atttgccgag gcgatgaaaa aagcagaaca taccattcac
      361 ttcaacggct acgtggatga aaccggttcc aatggtgtcg aatggcatat ccccgcaagc
      421 cacttcctcg aagcatgggg tgatgtgcgt tctgctaacg gtacggtcag cgcgattcag
      481 cctttgattg atccgttgta tgcaacacat tctgacattg aagtgttgta cttacttacc
      541 acgggtaaag aagcgcgcgg gtatgatatc gtacgcgaaa catggaaaac actctttgcc
      601 aaaggcttgg attttgagaa agcatggcgt ttggcattgc atgatggcgt tgtcggtgat
      661 acggctacgg cccctgtaac cgtcaaaatg gacgctaaag ccgcgagtgc atttgttgca
      721 aaaaatgcgt tgccgtcagg atctgcggat gccaataact tagaaatcgt tttctatgca
      781 tcgtccgcta ctttcgacgg tcgttttgct aacaacggtt ggctgcaaga attgccggat
      841 ccggtgacga agatcacgtg ggataatgcg gctatcatga gccacaaaac agcagaagcg
      901 cttaaagtca aaaatattct cggcgaaggt aaaaatcacc agccgatgat tcgattgaaa
      961 tatcaagggc aaacgttgga acttcccgta tgggttcaac ccggtcatgc ggattattct
     1021 atcgctgttg ctttaggata cggacgccaa tcggccggcc gcgtgggaaa tggcgtaggc
     1081 gctgatacgt atgctctgcg cacatccgcc gccatggatt ttgatctcgg cttgacagcg
     1141 gagaaactgt ccaaaacgta tttgatcgca accgtgcagg atcacggtgg atttgatacg
     1201 gacgcgcttg ctgataatga aattcaacgt cgtatcccga ccattatccg ggaagcgacg
     1261 atcgaagagt acaaaaaaga tccgaatttt atcaccagcg gtcgtacgcc cggtgaaaaa
     1321 gaattgtacg gaaaagacgg caagccgatt tcgatcatga atatgcacga atacggcccg
     1381 gaacgatttg aaaaaggcga gtggaataaa aaatatcaat ggggtatgtc cattgacctc
     1441 aatgcttgca caggttgtaa tgcgtgtatg atcgcttgcc aaagcgaaaa caatattccc
     1501 gtggtcggta aggaagaagt ggccaagggc cgcgaaatgc actggattcg cttggatcgt
     1561 tactatatcg gtaaagatgt gaacgaaccg gaaatgcagt tccagccgat cgcttgccag
     1621 cactgcgaaa ccgcgccgtg cgaacaggta tgccctgtcg ctgcgacagt acacgatagc
     1681 gaaggtctca acgtaatggt gtacaaccgt tgtgtcggaa cccgatattg cgccaataac
     1741 tgtccctaca aagtacgtcg tttcaatttc ctcgaatttc atgccggtac gtccggattt
     1801 atggaatcga aaccgatcga atccctcaaa ttgggcaaaa accccgatgt gacggtgcgt
     1861 atgcgcggtg tgatggaaaa atgtacgtat tgcgtgcagc gtatcagcgg tgcccgtatc
     1921 gcggcgaaga aagaagaccg cgatatccgc gacggcgaag tcgtcacggc atgccagcaa
     1981 agctgcccga cgggagccat cgtttttggc aatatcaacg atcccgaaag caaggtgtcc
     2041 aagatgaaag ccatgaaccg caaatacgaa gtgttggccg aactcaatgt tcgtccgcgc
     2101 acgagcttcc tggcgaagct gaaaaatccg aatccggaat taacaaaaaa cgcttaacta
     2161 taaaacgagt aacgctgtga gtgtagcaat tcaagaacat catacggccg acgaaccgct
     2221 tatcacgagt aacgccacgt tctcgggtat aacgaatcaa gtcagtgccg tcgttgaaaa
     2281 caaaccgccg cgtccatggt atgtattttt tgccatggcc agctccggct ttctgctcct
     2341 gctgatcgcg atcacgtacc tgctttttga aggtacgggt atctggggta tcaccaatcc
     2401 tgtcggttgg ggtcttgcga tcgtaaactt tgtgttttgg gtcggtatcg gtcacgccgg
     2461 tacgctgatc tcagcgattc ttttcctttt tcgtcaaaaa tggcgcacgt ccatcaaccg
     2521 ctttgcggag gcgatgacga tttttgccgt tatctgcgcg ctcttattcc cgggtattca
     2581 cgtcgggcgt atgtgggcga cgtattggtt cgcaccggtt cctaatcaga tggggatgtg
     2641 gcctaacttc cgcagcccgc tgttgtggga ctttttcgcc gtcggtacgt attttaccgt
     2701 gtccctgatt ttttggtatg tcggcttgat accggatttg gccagcttgc gtgatcgcgc
     2761 caaaacgcgt gtccgtcagg ttgtgctcgg tttcttgtcc ctcggatggc gcggcggtaa
     2821 taagcagtgg aaacattatg aaatggcgta tttgatactc gccggtattt ccacaccgct
     2881 ggtgctctct gtgcacagta tcgtttctac cgacttcgcg acgtcgatca ttcccggatg
     2941 gcatacgacg atatttcctc cgtactttgt tgccggcgct attttctccg gcttcgctat
     3001 ggtaatgaca ctcgcgatcg tagcgcgtaa agcgtataag ctggaaaaca tcatcacgtt
     3061 gtaccacttg gagaacatga ataagatcat gctcctcacg ggtatgatgg tcggctatgc
     3121 gtacagttgc gaatttttca tcgcatggta cagcggtaat atgtatgaac gatttgcgtt
     3181 tatcaatcgc gctttcgggc cgtattggtg gtcgtatttc agtatgattt tctgtaacgt
     3241 cgtcgtaccg cagatttttt ggtctaagaa attacgtcgc aatatcacgg taatgtttat
     3301 agcttcgatc ttcatcaata tcggtatgtg gtttgagcgt tttgtgatca ttgtgacttt
     3361 gtcacgtgat tatctgc
//
LOCUS       DAOQNQ010000334         3376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_13840_length_3376_cov_5.470641, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000334 DAOQNQ010000000
VERSION     DAOQNQ010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13840_length_3376_cov_5.470641"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="PK754_14385"
     CDS             complement(<1..998)
                     /locus_tag="PK754_14385"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNB10737.1"
                     /translation="MVSTRSSKLIAGISLIIIGIVAGVILTANFNFTHKGKASAESEQ
                     RVKLEETMATMKDFSEGFSAVAEYVTPSVVTVETEKTVRVDSRQFNPFGDFFGDNDPF
                     QQFFGAPRGGQPQEQKLSGLGSGVIVSEDGYIITNNHVIDNTDKIKVSLSNEKTYDAK
                     LIGTDPRTDIAILKIDEKGLPAVKIADSDKIKVGQWAVAIGNPFSKQLSHTVTAGIVS
                     GIARSSINSDTDVDFLQTDAAINPGNSGGALVNMSGELIGINAAILSKSGGYEGIGFA
                     IPSNVAKSVMDQLIKNGKVVRGYVGVQMQDISEEMAKALSLKEPKGAVVAAIMEDSP"
     gene            complement(1108..1974)
                     /locus_tag="PK754_14390"
     CDS             complement(1108..1974)
                     /locus_tag="PK754_14390"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNB10738.1"
                     /translation="MKDYYKILGVSESASSEDIKTAYKNLAKKYHPDRNPGNKQSEEK
                     FKEMSEAYNILSDVKKRKEYDTMRRFGGQGSGFSGSDNFDLNDFMHNFGFSGQNASFR
                     SSGGFSDLFDEMFFGKGGAHASEANQRVELTITFEKSIHGGEVDFTLRQHGNQNLRVK
                     IPAGIQDGEVLRIQNPNIGSINLTIRVQPDKAFTRKGIDIYADVQANLAQLMFGSSVR
                     VHTVYGNAIDVKIQPGTQPGTILKLSGFGVKTSRNHGDMYVTIKTKIPGHLNKHQKEL
                     FENFAKSLDLKW"
     gene            complement(1984..2871)
                     /gene="htpX"
                     /locus_tag="PK754_14395"
     CDS             complement(1984..2871)
                     /gene="htpX"
                     /locus_tag="PK754_14395"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HNB10739.1"
                     /translation="MKILNTFKTMFFLTTLVLLFGFIGMMIGGKSGMIIALILAGLMN
                     FVTYWFSDKIVLSMYGAKEVQYADQPKLYSMVQNLAQRAGLPMPRVYIIPTDTPNAFA
                     TGRDPQHGAVAVTDGIMARLSDDEMEGVIAHELGHIKNRDILISTIVATIAGAISSLA
                     SMAQWAAIFGGFNRSDDEDNGGGGNIVSAMIIAIVAPIAAMLIQLAISRSREFLADQA
                     GAEISGKPMGLANALIKLEQTNLRLPMDANQATAHLFIVSPLSGKGFVNLFRTHPTTE
                     ERVAKLKQWEEQQYGLITR"
     gene            complement(2988..3206)
                     /locus_tag="PK754_14400"
     CDS             complement(2988..3206)
                     /locus_tag="PK754_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020005916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNB10740.1"
                     /translation="MPTYDYKCKNGHVFEEFHGINDSPETVCPQCGQPAVKEIGAGSG
                     LMFKGSGFYITDYKKNSGSDSGSKSGKE"
BASE COUNT          857 a          846 c          702 g          971 t
ORIGIN      
        1 gggctatctt ccatgatcgc agccacaacg gcgcctttgg gttctttcaa actcagcgct
       61 ttggccattt cttcggaaat gtcctgcatt tgtacaccga catatccgcg aacgacttta
      121 ccatttttga tcaattggtc catcaccgat ttagcgacat tggaaggtat ggcgaacccg
      181 ataccttcat aaccgccgct tttcgataaa atcgcagcat taataccgat gagttctcca
      241 ctcatattca ccaatgcgcc gccgctattg ccgggattga ttgcggcatc ggtttgcaga
      301 aaatccacgt cggtatccga gttaatggac gagcgcgcaa ttccactgac gataccggct
      361 gttaccgtat ggctaagctg tttgctaaaa ggattgccaa ttgcgacggc ccactgcccc
      421 actttgattt tatccgaatc ggcaatcttt acagcgggta aacctttttc atcaattttg
      481 agaatggcta tatccgtgcg cggatctgta ccgataagtt tggcatcgta agttttttca
      541 ttggataaac ttactttgat tttatccgtg ttatcaatca cgtgattgtt ggtaatgata
      601 tacccgtctt cgctgacaat aacaccagat ccgaggccgc tcaatttttg ttcctgaggt
      661 tgtcctccgc gcggagcgcc aaagaactgt tgaaagggat cattatcgcc aaaaaaatca
      721 ccgaacggat taaattgacg ggaatctacg cgaacagttt tttctgtttc gaccgtgaca
      781 acactcggag taacgtattc tgcaacggcc gaaaatcctt cactgaaatc cttcattgtc
      841 gccatcgttt cttccaattt gacgcgttgc tcggattccg ccgaggcttt gcctttatgc
      901 gtaaaattga aattggccgt caaaatcacg ccggcgacaa taccgataat gatgagggaa
      961 attccagcaa tgagttttga tgaacgtgtg ctaaccatgg ttaagacctc cattcgttgt
     1021 gcgatgttgt ttacgattac tatttacgca caacgtcatg tcaaagtttt gttaattttt
     1081 tttaatcaat gtaaaatctg aaaattgtta ccactttaaa tccaaactct tagcaaaatt
     1141 ttcaaaaagc tccttttgat gtttgttcaa atgtcccggt atttttgttt tgatcgtaac
     1201 atacatatcg ccatgattgc gcgaagtttt aacaccaaaa ccggaaagct tcaatattgt
     1261 accgggttgt gtaccgggct gtattttgac atcgatcgca ttgccatata cggtgtgtac
     1321 acgtacgctg ctcccaaaca tcaactgcgc aagattggcc tgcacatccg cataaatatc
     1381 aattcctttg cgagtaaatg ctttatccgg ttgcacgcga atcgtcaggt taattgaacc
     1441 gatattcgga ttttggattc gcaacacctc gccgtcctgt atgcccgcgg ggattttaac
     1501 gcgtaaattt tggttaccat gttgccgcaa cgtaaaatcc acttcaccgc cgtgaataga
     1561 cttttcaaat gtaatggtca attcaacccg ctggttcgcc tctgaagcat gtgcgccgcc
     1621 ttttccaaag aacatttcat caaataaatc cgaaaaaccg cctgaactgc gaaaagacgc
     1681 gttctgtccg ctaaatccga aattgtgcat gaaatcattc aggtcaaaat tatcacttcc
     1741 cgaaaagccg ctaccctggc cgccaaaacg tcgcatcgta tcatattctt tacgtttttt
     1801 gacatcgctt aaaatattgt acgcctcgga catctcctta aatttttcct cggattgttt
     1861 attacccgga ttacggtcgg gatgatattt tttggcgaga tttttatacg ccgttttaat
     1921 atcctccgac gaagcggatt ccgacacacc gagtatttta taataatctt tcatcgttac
     1981 gcattaacgg gtaataagtc cgtactgctg ttcttcccat tgtttgagtt tggcaacacg
     2041 ctcctcggtc gtcgggtgcg tgcgaaacaa attaacaaac cctttgcccg aaagcggact
     2101 cacaataaac aaatgtgccg tagcctgatt ggcgtccatg ggcagacgca gattggtctg
     2161 ctccagttta atcagagcat tagccaaacc catcggttta ccggatattt cagcaccggc
     2221 ctgatcggct aaaaattcgc gcgaacgcga aatggctaat tgtatcagca tcgctgcgat
     2281 cggagccact atggctatga tcatagcact cacgatatta ccgccgccac cattatcttc
     2341 gtcatcggaa cggttaaacc cgccgaaaat agccgcccat tgggccatac tggccaagga
     2401 ggaaatcgct cctgcgatcg tggccacgat ggtactgatc aaaatatccc ggtttttgat
     2461 gtgaccgagt tcatgcgcaa taacgccttc catttcatca tcactcaaac gggccatgat
     2521 accatcggtc accgctacgg caccgtgctg ggggtcgcgc cccgtagcaa aagcattggg
     2581 cgtatcggta ggaatgatgt acacgcgcgg catcggcaaa ccggcacgct gagcaagatt
     2641 ttgtaccatg ctgtacaact tgggttgatc ggcgtactgc acttctttag cgccatacat
     2701 gctgagtacg atcttatcac tgaaccaata cgttacaaaa ttcatcaacc ccgccaaaat
     2761 caaggcaatg atcattccgc ttttaccgcc gatcatcatg ccgataaaac caaaaagcag
     2821 aacgagtgtt gtgaggaaga acatcgtttt gaacgtgttc aggattttca taggttttca
     2881 cctgtcattt tagttgcacc gtcgtccgta aacccacgcc ggtaaatgat ttgaactgca
     2941 ctttagcatg agtttttacg gatgccgaag tgcttactca aaaatcgtta ctctttgccc
     3001 gactttgatc cgctgtccga gccgctgttc tttttatagt cggtaatata aaacccggaa
     3061 cctttgaaca tcaggcctga accggcgcca atttctttta ctgccggttg tccgcattgc
     3121 gggcaaaccg tctcagggct atcattgata ccatgaaatt cttcgaaaac atggccgttt
     3181 ttgcatttat aatcgtatgt cggcatacaa attatctttc agattacgaa atgttgatga
     3241 ttagttaatc aagttacttc ttattttcat ccaccacttc ataatcggcc tcttcgactt
     3301 tttggccgct ctgctgttgc gcaccaccgt ctgtcggacc tgcaccggtc gcgccctgtg
     3361 caccggcgcc cgcctg
//
LOCUS       DAOQNQ010000335         3376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_13841_length_3376_cov_5.406504, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000335 DAOQNQ010000000
VERSION     DAOQNQ010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13841_length_3376_cov_5.406504"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PK754_14405"
     CDS             complement(<1..406)
                     /locus_tag="PK754_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10741.1"
                     /translation="MRLRFLLCTVLMLSYSFDVLAQEQRPSGPSKKKAIILSLLAPGT
                     GELYLSDWKFEQWGSGKYFAATEVLFWATNFYTRSYSGWLKEDARALAARKAGVDIRS
                     TKPAKYYVNIGKYRDIYTYNETQRRATGTEFLY"
     gene            complement(403..1833)
                     /locus_tag="PK754_14410"
     CDS             complement(403..1833)
                     /locus_tag="PK754_14410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB10742.1"
                     /translation="MLRRIRAKIQNKLIVTFAAFCLTIFSASGIIHYWYTESSLDEEL
                     GKKLLAVAQAAKLQIHTEMVSKLESGDENSRTYRTFKQKLTDLRDATDVERIYIIDPN
                     GRSLMDTEEGVRIGYAYPFLKFNQYEFERAAAGLPSHSILFETYDGKLYKTAFVALTV
                     NGQTHGVIAVDGSATFLYLMKRIEWHLITVGIIGVVVSVAIGFYFSRSISTPIKQIMV
                     EAEHIGSGKLDRKIAIQTGDEVGFLGRTMDRMRENILKRDQYMKTMLAGVAHELRNPL
                     GGMELYAHLLNKEIPDGDNRKKAILKVLRELDVMKQIVNDFLDYARPKEPQPMVCDLS
                     KLIDEIRINLSALTTEKSVQWEVELNEKQVYADPGHLRQILINLIENAMQSGNVLPRV
                     RIVSQRVNAMIEITVEDNGHGIEDNIKEKIFDPFFTTKEKGSGLGLAIVKKFVDENDG
                     DITITSSGTNGTMVRLMFPTKEGVSA"
     gene            complement(1912..2799)
                     /gene="xerD"
                     /locus_tag="PK754_14415"
     CDS             complement(1912..2799)
                     /gene="xerD"
                     /locus_tag="PK754_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017435275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNB10743.1"
                     /translation="MKHHLQDFIDYLSVEQNVSPNTLASYRNDLERYLKFVMGSRERK
                     KIEDVTMQDVRELVQTLDGLGLTAKSIARNLSAIRTFHKFLLQEEILENDPAELIDLP
                     KIEKTLPSVLEVPEIETLLEQPDTSSRLGIRDRAMIELLYACGMRISELLTIKQLDIY
                     SDYVRVFGKGSKERIVPMGDTAGYWVTYYREKVRPHLAASGSADEVLFLNAQGKRMSR
                     MGFWKILKKYVTMAGIQKEVTPHTFRHSFATHLLEGGADLRVVQELLGHSDISTTQIY
                     THIDREYLKEVHKTFHPRG"
     gene            complement(3102..>3376)
                     /locus_tag="PK754_14420"
     CDS             complement(3102..>3376)
                     /locus_tag="PK754_14420"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNB10744.1"
                     /translation="PLRTYYDFGLRVTVNTDNRLISGTTVTKEIYRAAKTFDLSFDDV
                     CNIIINGFKSTFLPYRERVVLLNDALAELQSFEFESSYIKDKAQAN"
BASE COUNT          918 a          827 c          697 g          934 t
ORIGIN      
        1 catataaaaa ctccgtcccc gtggcgcggc gctgcgtttc attgtacgtg tagatatcac
       61 gatacttccc gatattcaca taatattttg ccggtttagt tgatcggata tctacgccgg
      121 cttttcttgc cgccaatgca cgagcgtctt ctttaagcca tccgctgtac gaacgcgtat
      181 aaaaattagt agcccaaaac aatacttccg tcgcggcaaa atacttaccg cttccccatt
      241 gctcaaattt ccaatccgat aaatacaatt cacccgtacc gggcgctaat aaggataata
      301 taatcgcttt cttttttgat gggccagatg gcctttgctc ttgcgccagt acgtcaaaac
      361 tatacgacaa catcagtact gtacacaaca aaaaccgtaa cctcatgctg acaccccttc
      421 ttttgtcgga aacatcaaac gcaccatcgt accattcgtt ccactcgatg taatggttat
      481 atccccgtca ttttcatcta caaatttttt tacgatggct aaacctaaac cggagccctt
      541 ttctttcgtc gtaaagaacg gatcaaagat tttctctttt atattatcct cgatgccatg
      601 tccgttatcc tcgacggtaa tttcgatcat cgcattgaca cgctgcgaca cgatacgcac
      661 acggggcaac acattacctg actgcatggc attttcgatc agattgatca aaatttgccg
      721 caagtgcccc ggatccgcat aaacttgttt ctcattcaac tcgacttccc actgtaccga
      781 tttttccgtc gtaagggccg aaagattgat tcgaatttca tcaataagtt tggacaaatc
      841 acagaccatc ggttgcggct ctttaggtcg tgcgtagtct aaaaaatcat tcacgatttg
      901 tttcattaca tccaactcgc gcaagacttt gagaatagct ttcttgcggt tatcgccgtc
      961 cggaatttct ttattcagta aatgggcata caattccatc ccgccaagcg gattgcgcaa
     1021 ttcatgcgct acgccggcca acatagtttt catatactga tcgcgtttaa ggatattttc
     1081 acgcatgcga tccatagtac gtcccaaaaa acccacctca tcgccggttt gaatagcaat
     1141 tttgcgatca agctttccgc tgccgatatg ttccgcttcg accataatct gttttatcgg
     1201 cgtagaaata gagcgtgaaa aataaaaccc gatagctacg gacacaacca caccgatgat
     1261 accgacggtg attaaatgcc attcgatgcg cttcatcaaa tataagaacg tcgcgctgcc
     1321 gtccacagcg atgacgccat gggtctgccc gttcaccgtg agcgccacaa aggctgtttt
     1381 atataatttt ccgtcatatg tctcaaacaa tatggagtgc gatggcaatc cggcggccgc
     1441 gcgttcaaat tcgtattgat taaacttgag aaaaggatac gcatacccga tacgtacacc
     1501 ttcctccgta tccataagac tccggccgtt gggatcaatg atataaattc gctcaacatc
     1561 ggtcgcatcg cgcagatccg tcaatttctg tttaaacgta cggtaagtgc gcgaattttc
     1621 atcgccggac tcaagcttgg aaaccatttc ggtatgtatc tgaagtttgg cggcctgcgc
     1681 gacggccagc agtttcttcc cgagttcttc atcaagactg ctctcggtgt accaataatg
     1741 aataattccg ctggcgctga atatggtaag gcaaaacgcg gcaaacgtaa cgatgagttt
     1801 attttgaatt ttagcgcgta ttcggcgtaa catagatgcg caatctatcc gatttctccg
     1861 ctaatggcaa gcgatttaaa agtgcagtac gcattatcag aataataata cctatcctcg
     1921 cgggtgaaat gtcttgtgca cttctttgag gtattcgcga tcaatatgcg tatagatctg
     1981 tgtggtcgag atatccgaat gaccaagcaa ctcttgcacg acacgcaaat cggcaccgcc
     2041 ctcaagaaga tgcgttgcaa aggagtgacg aaatgtatgc ggcgtaacct ctttctgaat
     2101 gccggccatc gtgacgtatt tttttaagat tttccaaaac cccatccggc tcatccgctt
     2161 accttgcgca ttcaaaaaaa gtacttcgtc cgccgatccg ctcgccgcaa ggtgcggacg
     2221 cactttctcc cgataatagg ttacccaata tcccgcggta tcgcccatag gcacaatacg
     2281 ttctttggag cccttgccaa atacccgaac gtaatccgaa taaatatcca actgcttgat
     2341 cgtcagtagt tccgagatgc gcataccgca cgcgtatagc aattcgatca ttgcgcggtc
     2401 tcggataccg agccgggacg aggtgtccgg ttgctcaagc aacgtttcga tttccggcac
     2461 ttccagtacc gaaggtaagg ttttttcgat tttgggaagg tcaatcaatt cggcaggatc
     2521 gttttctaga atttcttcct gtaaaagaaa tttatgaaac gtacggatgg cggataagtt
     2581 acgggcgata ctttttgctg taagcccaag gccatccagc gtttgcacaa gctcacgcac
     2641 gtcttgcatg gtaacatctt cgattttttt tcgttcgcgt gatcccatga caaacttcag
     2701 atagcgttcc aaatcgttac gataagaagc gagcgtattc ggtgaaacgt tttgttcaac
     2761 cgaaagataa tctatgaagt cctgaagatg gtgtttcata atcgcttatt tacacatgaa
     2821 aagcatccca tgcgattgta atcaggtttt ttaagagatc aaaacaaaaa caaggatggg
     2881 tagtctattc tacgataatc gtttggaaaa gaagatgtaa acagaattct tggaatgctt
     2941 tacagcttga cgaaccgacc ttgcaacacg gcaccgtctc atttgtaaat ctgcgtcatc
     3001 acacgatcat cgtgatgata cgccgacaag cagcagaatg gtataacggt ttttagccta
     3061 agttagaacg cacttccatg tggcggcaga ctaatactta cttaattagc ctgcgcttta
     3121 tctttgatat agctggattc aaattcaaag ctttgcagtt cggccaacgc atcattgagc
     3181 agaacgacgc gctcacggta tggaagaaat gtacttttga agccattgat aatgatgttg
     3241 caaacatcat caaacgacag atcaaaagtt ttcgccgcac gataaatttc tttggttacg
     3301 gttgtaccgg aaatcaatcg gttatccgta ttgaccgtga cgcgcaaacc gaaatcataa
     3361 taggtgcgca acggat
//
LOCUS       DAOQNQ010000336         3344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_14077_length_3344_cov_4.508057, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000336 DAOQNQ010000000
VERSION     DAOQNQ010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14077_length_3344_cov_4.508057"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PK754_14425"
     CDS             <1..435
                     /locus_tag="PK754_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10745.1"
                     /translation="IAVGRFGQNINLAARLTGFQIETIKYSELSGEAHVEETEDEIVD
                     ENETPEVETIRETTVKPTVRNEAKPEAPPEVYTEASLIVEVRGISKAVAEKLLVAGFV
                     KLGDIASIEEIKNVEGVPERAISRLAAVIDNLQPATETSSNE"
     gene            465..3041
                     /gene="infB"
                     /locus_tag="PK754_14430"
     CDS             465..3041
                     /gene="infB"
                     /locus_tag="PK754_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNB10746.1"
                     /translation="MSKKVFQLAKELLVSHQEIVTFLQKSGFKEVKNHMSGLDEEMIE
                     KVMAKFSKEKKHADTYQRIKDKKEKKDEKQTSIFELSSEPKPEPELEPESVKDEKTVK
                     EVSEPATVHQTEPVPAEPHAVPADETISVEAKNTNADTHPSEKKKVDLLENLKSSQKS
                     IEEFKLAFEKARQQLEKQAMKDETDPRRKKKKGGKEEGEAEPEVLVVEEKGGKNNKKK
                     KKKKNKIDEKDVEAAIRETMAKMDDSRTAGEERQKRRKQKREEREAEAAESEEERNVI
                     RVHEYLSAHELADMMDVAVGEIIKTCLQLGLMVSINQRLDMDTITLVAGEFGYDVEQV
                     KEFEENEIEELEEQEDVPESTRAPIVTIMGHVDHGKTSLLDYIRKANVVAGEAGGITQ
                     HIGAYEVTLPSGRKITFLDTPGHEAFTAMRARGAQVTDIVVIVVAADDNVMPQTVEAL
                     NHAQAAGVPIIIAINKIDKPEANSMRIKTQLSEKNILVEDWGGKYQCVEISAKKGLHV
                     DTLLEKILLEADVLELKAKVECRAKGTIVESQLDKGRGATATALVESGTLRIGDPFIC
                     GIYYGRVKAMFDERGNSVKAAGPSTPVQLIGFDGLPQAGDKLVVTSNERIARNIATKR
                     HQLKREQDHRRVKFLTLDEISKQVRDGKVSDLAIVLKGDVDGSVEALSDSIQRLSTPE
                     VKVNIIHRGVGVITESDVLLASASGAIIIGFHVRPNLNARKLAERENIDIRYYNIIYD
                     CIEDIRKALEGMLEPDYEIKITGMVEIREIFKISKVGTVAGCYVVDGKVNRTQPIRLL
                     RNGEVVHEGKLSSLKRFKDDAREVAQGFECGLTIDGYNDIKSGDVVECYERVETKRTL
                     SV"
     gene            3055..3336
                     /locus_tag="PK754_14435"
     CDS             3055..3336
                     /locus_tag="PK754_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018035034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HNB10747.1"
                     /translation="MIVGVFKLDLFIHASASLKEKRFVVKSIKDGIRHKFNVSIAETG
                     HLDKWQRAELGISMVANEEHLIRSTMEAVMLFIEAKGQCEMVNRELTMY"
BASE COUNT         1067 a          706 c          836 g          735 t
ORIGIN      
        1 atcgcggtcg gccgctttgg tcaaaatata aacttagccg cacgtctgac cggttttcaa
       61 atcgaaacaa ttaaatacag cgaattgagc ggcgaggcgc acgtcgaaga aacagaggac
      121 gaaatcgttg acgaaaacga aaccccagaa gtcgaaacaa ttcgtgaaac tacggttaag
      181 ccgacggttc gcaatgaggc taaacccgag gcgccgcctg aagtttatac agaagcttcg
      241 ttgatcgtcg aagtccgagg catatccaaa gcagtagccg aaaaattact ggtagcaggt
      301 ttcgtaaaac ttggtgatat tgcttccatc gaagagatca aaaacgtcga aggagttccc
      361 gaacgtgcca ttagtcggtt ggccgctgtt attgataacc tgcaaccggc gacggaaacg
      421 tcttccaatg aataagtatt tcttaactac ataaaagcgg atttatgtcc aaaaaagtat
      481 ttcagcttgc caaagagctt ttggtaagcc atcaggaaat cgtaacattt cttcaaaaga
      541 gcggcttcaa agaagtaaaa aatcacatga gcgggctcga tgaggaaatg atcgagaagg
      601 taatggctaa gttttccaaa gagaaaaaac acgccgatac ctatcagaga ataaaagaca
      661 aaaaagagaa aaaagacgaa aaacaaacgt ctatttttga gctgtcatcc gagcctaaac
      721 cggaaccgga attagaaccg gaatccgtaa aagatgaaaa gacggttaag gaggtttcag
      781 aacccgcaac agtgcatcag actgaaccgg tgccggctga gcctcatgct gtgcctgccg
      841 atgaaacgat ctcggttgaa gcaaaaaata caaacgcgga tacgcatcct tcagaaaaga
      901 aaaaagttga tctgttagaa aacctgaaat catcgcaaaa atccatcgag gaattcaagc
      961 tcgcgtttga aaaagcgcgc caacagcttg aaaaacaagc gatgaaagac gaaaccgatc
     1021 cgcgtcgtaa aaagaagaag ggtggcaaag aagagggcga agcggaacct gaagttttag
     1081 tggtcgagga aaaaggcggt aagaataata aaaagaaaaa gaagaagaaa aataagatag
     1141 acgaaaaaga cgtcgaagcg gctatccgtg aaacgatggc taagatggat gattcgcgca
     1201 cggccggcga agaacgtcaa aaacggcgta agcaaaaacg cgaagaaaga gaagcagaag
     1261 cggcagagtc cgaagaagaa cgtaatgtga ttcgtgtgca tgaatatctt tcggcgcacg
     1321 aattagccga tatgatggat gttgccgtag gcgaaattat taaaacctgt ctccagcttg
     1381 gattgatggt ttctatcaat caacgccttg atatggatac cattacgctc gtagcgggtg
     1441 aattcggata cgatgtcgag caagtcaaag agttcgagga aaatgaaatt gaagagttgg
     1501 aagagcagga agatgtaccg gaatctaccc gtgcgccgat cgtaacgatc atgggtcacg
     1561 ttgatcacgg taaaacatcg cttcttgact atatacgtaa ggcgaacgtc gtagctggcg
     1621 aagcaggcgg gatcacccaa catatcggtg cttacgaagt tacgctccct tcaggccgaa
     1681 agatcacatt tttggataca ccgggccacg aagcctttac cgctatgcgt gcgcgtggtg
     1741 cacaggttac ggatatcgtc gtgatcgtcg tcgcggctga tgataatgtc atgccccaaa
     1801 cggtggaagc ccttaatcac gcgcaagccg cgggcgtacc gatcattatc gcaattaaca
     1861 agattgataa gcccgaagcc aactctatgc gtattaaaac gcaactttct gaaaaaaata
     1921 ttcttgtcga agattgggga ggtaaatacc aatgtgtgga aatctccgct aaaaaaggtt
     1981 tacacgtaga tacattattg gagaagattc tcctcgaagc ggatgttttg gaactcaaag
     2041 cgaaagtaga atgccgcgcg aaaggaacca ttgtggaatc acaattggac aaaggacgcg
     2101 gagcgacggc cacagcgttg gttgagtcag gtactctacg catcggcgat ccgtttattt
     2161 gcggtattta ttacggtcgt gtgaaagcta tgtttgatga acgcggtaat tcggtaaaag
     2221 ccgccggtcc ttcaacaccg gtgcagctta tcggctttga cggtttaccc caagccgggg
     2281 ataaattggt tgttacctcc aacgaacgta ttgcccgtaa cattgccaca aaacgtcatc
     2341 agctcaagcg cgagcaggac catcgccgcg ttaaattcct taccctggat gaaatctcta
     2401 aacaagtgcg cgacggcaaa gtatcggatt tggcgatcgt actcaaaggc gatgtggatg
     2461 gttccgtcga agcactcagt gattcgattc aacgcctcag tacacccgaa gtgaaggtca
     2521 atatcattca ccgcggcgtc ggcgtaataa ccgaatcgga tgtgttgctc gcatcggcct
     2581 cgggagcgat catcatcggt ttccacgttc gtcccaatct caatgcccgt aaactggccg
     2641 agcgtgaaaa catagatata cgctattaca acattattta cgactgtatc gaagatatcc
     2701 gcaaagcgct cgaaggtatg cttgaaccgg attatgaaat taaaatcaca ggtatggtcg
     2761 aaatacgtga aattttcaaa atatccaagg tgggtacggt tgccggctgt tatgtggtgg
     2821 acggaaaagt caatcgtaca cagcccattc gtcttctgcg caacggcgaa gttgtacacg
     2881 agggcaaact ttcatcactc aagcgtttca aagacgatgc ccgcgaagtt gcgcagggat
     2941 ttgagtgcgg tcttaccatt gacggataca atgatatcaa atctggtgat gtcgtcgaat
     3001 gctacgaacg cgtagaaacc aaacgaacat tatcggtatg agcaccccac gatcatgatc
     3061 gtcggagttt ttaaattaga tttatttata cacgcaagcg cttcactcaa agagaagcgc
     3121 tttgtcgtaa aaagtattaa agacggcatt cgccataaat ttaatgtcag tattgcagaa
     3181 acaggccatc tggataaatg gcaacgcgcc gaattgggca tcagtatggt ggccaacgaa
     3241 gagcatttga tacgcagtac gatggaagct gttatgcttt tcattgaggc caaaggccaa
     3301 tgcgaaatgg taaatcgcga actgacaatg tattaaaaag agag
//
LOCUS       DAOQNQ010000337         3334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_14145_length_3334_cov_4.773711, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000337 DAOQNQ010000000
VERSION     DAOQNQ010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14145_length_3334_cov_4.773711"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3018
                     /locus_tag="PK754_14440"
     CDS             <1..3018
                     /locus_tag="PK754_14440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNB10748.1"
                     /translation="WTNKTNWKSGNPIDTWFGITVTDGRVSHVELGNNNLSGFIPSSV
                     GNISAIKNLNLFNNNVGGPIPATISALTEIVSLNLQNNQLTGSIPASITALTKLKHLY
                     LDFNTLSGAIPSDIDNLTDLETLSLYQNQLSGTIPGGIWNLHKLNHLALGPNPGLSGT
                     LPAQIENLDSLQSLFISSCGLTGSIPPEIGNLNKILMIYASDNQFTGTIPASIGNLSL
                     LRTLHLANNNLSGSLPSSLANLHRLESLDIKENKFTDLGDLNPVDSLNVLMIEQNQFT
                     FEDIEPNITAPRQSFTYAPQDSIGTYKDTAVTENAATSLAVSVGGSGNQYLWFKDGSS
                     TGITTPVISFGSFAFANAGTYHCQITNTVATALTLYSRTYTVKLRGPSIPTGLTASAQ
                     SASQINLSWSASQNNPIRYRIYRSPTSGSGFVQVDSVSHPTVAYNNTGLTADTDYYYR
                     ITAVDAAGLYSAATTQASARTLQTAVSITSVSLLVGPTQPNENTIVTISVPVSGSAPV
                     VKLYYAKNSSVNPDSTEMTLSSGPYVASIPASFVTAQGLWFRIRAYNNTGSEFYPSST
                     AKHPINVKAGASTLSAIRSSGAYPSGMIADGYSTFGLPMDTSVGIPALFGSQIFNSKN
                     EPTNWRIQKYADGSFTDETSAFTSGTGYFVYHRLGSSVQVDIPPGSLTHAAGTFENFV
                     LKPGWNLVPWPYTFSANFTITDESAIDPIQTQIGGNWQIVSSFKPFGAYAIKNTTASD
                     ILAGDVIQWTLSTSKVTVDYDLISDSFVRLSAVSGRYLDNDNFAGFSDAARDGDDPLD
                     NEEPPAIGPGVNLYFTNGTEQRQYADIRSRGDGHVWDFHVNDRNNGKRINLAWDLAHI
                     PDRMSAVLINIAENKWINLGEQTSYAFSPVKENRFKLIIGTSDFVQQTSIALAKELPS
                     SFALHQNHPNPFNPSTTIRYDIAHAGHVRIKIYNLLGQEIVTLVDQFVNTGRYSKLWD
                     GRDRLGNKVASGIYIYRLETSKFVRSKKMLLMK"
     gene            complement(3105..>3334)
                     /locus_tag="PK754_14445"
     CDS             complement(3105..>3334)
                     /locus_tag="PK754_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10749.1"
                     /translation="MKTRRWDDKSGGKHALTEVVAETMQMLGSRSSKQNVHDDDASDE
                     TGIETHDDVAEIVSENEYSEHTVVRTDEVPF"
BASE COUNT          863 a          861 c          735 g          875 t
ORIGIN      
        1 tggacgaata aaacaaactg gaaatccggc aaccccatag atacatggtt tggcatcacg
       61 gtgaccgatg gtcgcgtctc gcatgtcgaa ctcgggaata ataatctgag cggatttata
      121 ccgtcatccg tcggtaatat ttcggccatc aaaaatttaa acttatttaa caacaacgtc
      181 ggcggtccga tcccagccac gatcagcgca ctcacagaga tagtatcttt aaacttgcaa
      241 aacaatcaac tgaccggcag tatccctgcg tccatcaccg cactcaccaa actgaaacac
      301 ctgtatcttg atttcaatac cctcagcgga gcaataccgt cggatattga taacctcacc
      361 gatctggaga ccttgtcatt atatcaaaat cagttgtccg gcaccattcc cggcgggata
      421 tggaatctgc ataaattgaa tcacctcgcg ttggggccta atcccggtct gagcggaact
      481 ctccctgcgc aaatcgaaaa tctcgacagc ctgcaaagcc tctttatttc ctcctgcggt
      541 cttacgggaa gcataccgcc ggaaatcgga aacctgaaca aaatcctgat gatttacgcc
      601 tcggataatc agttcacagg caccataccg gcatccatcg gaaacttgag tttgcttcgt
      661 acactgcatc tggcaaataa taatctttcc ggaagtttac cgtcttcatt ggctaatctg
      721 catcgtctgg agtcgctcga catcaaagaa aataaattca ctgatcttgg agatttgaat
      781 ccggtggatt cgctcaacgt gctgatgata gagcaaaatc aatttacatt tgaagacatc
      841 gaacccaaca taaccgcgcc ccgccagtcc tttacgtatg cccctcagga cagcattggc
      901 acatacaaag atacggcggt tacggaaaac gcggcgactt cgttggccgt cagtgtcggc
      961 ggctccggta atcaatatct ttggttcaaa gacggttcct ctaccggcat tactactccg
     1021 gtgataagtt ttggttcttt tgcatttgca aatgccggca cgtatcattg ccaaatcacc
     1081 aatacggtgg cgacggcact gacattatat tcgcggactt acaccgtaaa actccgcgga
     1141 ccgtcaattc ccacaggtct caccgcgtcg gcccaatccg catcgcagat caatttatca
     1201 tggagtgcaa gtcagaataa tcctattcgc taccgcattt atcgcagtcc cacatcgggc
     1261 tccggttttg tgcaggttga ttccgtttcc catccgaccg tggcgtacaa taatacagga
     1321 ctgactgcgg atacggatta ttattatcgc atcacggctg ttgacgccgc cgggttatac
     1381 tccgcggcga ccacacaagc atcggcgcgt acgttgcaaa cagcggtttc cattacttct
     1441 gtttcattgt tagttgggcc cacacagccc aatgaaaaca ccatagtcac catttccgtt
     1501 ccggtatcgg gttctgctcc ggtcgtaaaa ttatactacg ccaaaaattc gtccgtcaat
     1561 ccggactcga ccgaaatgac actgagcagc ggtccatacg tcgcaagcat tccggcttcg
     1621 tttgtgacag cccagggatt gtggtttcgc atccgcgcct ataataacac gggttcggaa
     1681 ttttatccgt cttcgaccgc caaacatccg atcaacgtca aggccggtgc gtcaacgctc
     1741 tcggccatcc gatcttccgg cgcttacccg tccggcatga tcgccgacgg ttactcgacc
     1801 ttcggtttac cgatggatac cagcgtcggc atccctgcat tgtttggttc acagattttt
     1861 aacagcaaaa atgaacccac caactggcga atccaaaaat atgccgacgg cagttttacg
     1921 gatgaaacgt cagcttttac atcgggcacg ggatattttg tatatcaccg gctcggctct
     1981 tccgtccaag tagatattcc tccgggttcg ctgacacatg cggccggtac atttgaaaac
     2041 tttgtgctta agccggggtg gaatctcgtt ccgtggccgt acaccttttc cgctaatttt
     2101 acgataaccg atgaaagcgc gatagatccc attcaaacgc agattggcgg caattggcaa
     2161 atcgtatcgt cattcaaacc ttttggcgct tacgctatta aaaatactac ggcttcagat
     2221 attttagccg gcgacgtaat ccagtggacg ctttcaactt ctaaagtgac ggttgattat
     2281 gatttgatat ccgatagttt tgtccggtta agcgcggtat cgggccgata tttggataat
     2341 gataattttg ccggtttttc tgatgcggcg cgggacggcg atgatccttt ggacaacgaa
     2401 gagccgccgg caatcggacc gggcgtgaat ctttatttta caaacggtac cgaacaacgc
     2461 caatacgcgg atattcgttc acgcggtgac ggtcatgtgt gggattttca tgtaaatgat
     2521 cgcaataacg gaaagagaat taatttagca tgggatttgg ctcatatacc ggaccgcatg
     2581 agcgccgtat tgataaatat cgcggaaaat aagtggatca atttgggtga acaaacatct
     2641 tatgcattca gtcccgttaa agaaaatcgc tttaagttaa ttatcggcac aagtgatttc
     2701 gtccaacaaa catccatcgc tcttgctaaa gaattaccct cttcctttgc cttacaccaa
     2761 aatcatccta atccttttaa cccgtccacg acgattcgtt acgatatcgc ccacgccggg
     2821 catgtacgta tcaaaattta taacctgctc ggtcaggaaa tcgtaacgct tgttgatcaa
     2881 tttgtaaata ccggccgtta ttccaaatta tgggacgggc gtgatcggtt gggaaataaa
     2941 gttgccagcg gtatatatat ttaccgcctc gaaacttcca agtttgttcg cagtaaaaaa
     3001 atgctgttga tgaaataaag cctacgatgc aaaaacaaaa accccgaccg attggccggg
     3061 gatttttgtt acctgtgaaa ggagttatgg aaggataatt aagatcagaa gggtacttcg
     3121 tcggtacgga cgaccgtatg ttccgagtac tcattttcag aaacgatttc agcgacatcg
     3181 tcatgggttt cgatcccggt ttcatccgat gcatcgtcat catggacgtt ttgttttgac
     3241 gatcggctgc ccaacatctg catcgtttcg gcgaccacct ccgtcaacgc atgtttaccc
     3301 ccgctcttgt catcccaccg acgtgttttc atcg
//
LOCUS       DAOQNQ010000338         3320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_14257_length_3320_cov_4.132925, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000338 DAOQNQ010000000
VERSION     DAOQNQ010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14257_length_3320_cov_4.132925"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PK754_14450"
     CDS             <1..589
                     /locus_tag="PK754_14450"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNB10750.1"
                     /translation="TKRELMIEMAAVVAFSAIKNNDKGGLMIYTEEVEKFIPPKKGRS
                     HVLRLIRELIAFVPKARGTRIAAPLELTSHILKRRSIVFVISDFLDQGFDTSLRTLAK
                     KHDVIGLRVADQREQNWPLVGLVTLEDEETGALIDIDTADQQFHAILRQRVDAEENRL
                     KEIFKSSRVDLINIFTGSDYAEALISFFRKREKRH"
     gene            746..1543
                     /locus_tag="PK754_14455"
     CDS             746..1543
                     /locus_tag="PK754_14455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB10751.1"
                     /translation="MKKIEWRVLFSVDGKRNVADIAEKLDRDATLVEEILQKLSAEKL
                     ISGGAPAPAAKPAKEKAPSKKAVKEDAPKAKAEPKPEPKAEPKPEPKIEKAPEPKPAP
                     KKEETDILGSMDTPKVEPKSEPKVEKAAPKPVAPAASASAGGRKILVVDDSIVIQKMV
                     EIALENDNYNLTSAMKGEDAINKAKEMQPSLILLDMMLPDMTGVEVMKALRETGGYFA
                     TVPIVVLSGKDSPQDKDAAISNGATDFLNKPFHDEDLLAKVHEYLGK"
     gene            1615..>3320
                     /locus_tag="PK754_14460"
     CDS             1615..>3320
                     /locus_tag="PK754_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10752.1"
                     /translation="MDNKSRKIVEFYLSELLNHVKSVSEHRFAPLEFSITKKIIVSIG
                     QAQEPVRQVESFLNVASLHPVATFLRSNLEKLEKSGYKMSQMITTLENDSERIIQMFQ
                     KILETPGSQDQLNNDLQTIGFSLDLPALRKTYIKQEDTPSPSEGTPKDEVKPISVGVK
                     TEPPASPAIKNDSIFSHNTSRAESTHEKTSVTSASPQSSLLEVAPITQSEAFDHVGDM
                     IGFLSRRTKKQPEKAVAQIVQPTEKVDPKENINAVRSVFSTDRLNSAWETLRDNPTRP
                     RFLISMADGLSQLRAEAVTQNAMPLSEATQRIEETLNAIADNNHKPLNPEALAVLHDL
                     IQQVENNNSDLKSLAPAEILRLQQRFTGSFNMEKPEESPVQSSSSNAQLVAENEIAID
                     RVAIQDDDWNVFAEDVRFNLELAQSALVRLKKATHDTEAIKDLLQVYRALSTNTRLLK
                     INLLHGHCDAVFQIFKQVVYSQSPVVAELITLVTRSTRSIEILVNGQTIQSDEWRWLN
                     DQSVRFSDYKQATTLVTPAKTTEISAVPSEQTKVVAPEVVKTEIKTAKESSKPISDPK
                     SVV"
BASE COUNT         1026 a          719 c          785 g          790 t
ORIGIN      
        1 gaccaaaagg gaactgatga tcgaaatggc ggcggtagtg gcatttagcg cgatcaaaaa
       61 taatgataag gggggcttaa tgatttatac ggaagaagtt gaaaaattta ttccgccgaa
      121 aaaaggccgt tctcatgtgt tgcgtctgat ccgcgagctg atcgcgtttg tacctaaagc
      181 acgcggtacg cgcattgcgg ctccgctgga gttgacatca cacattctca aacgccgcag
      241 tatcgttttt gtgatcagcg atttccttga tcagggtttt gatacctcct tgcgtacctt
      301 agctaaaaag cacgacgtga tcggtcttcg ggtcgccgat cagcgcgagc aaaattggcc
      361 tctggtcggt ctggtaacac tggaagacga agaaaccggg gcgttgattg atattgacac
      421 ggccgatcaa cagtttcatg cgatattgcg acagcgcgtt gatgccgaag aaaacagatt
      481 gaaggaaatt ttcaaaagca gtcgcgttga tttgatcaat atttttacgg gtagtgatta
      541 tgccgaagcg ctgatttctt ttttcagaaa acgagagaaa agacattgaa acttattgac
      601 ataatgattt atctttatta gatttacaaa gttattaaca cgatttgaat actcacaggt
      661 tcatcatcat aaagcggcga ggtatattgt ggatgcagtt tactccagag taaaagactt
      721 acgagaaaaa gttgagacca ttctgttgaa aaaaattgaa tggcgtgttt tgttttctgt
      781 tgacggtaaa cgcaatgtgg ccgatatcgc agaaaaactg gatcgcgatg caacgttggt
      841 cgaagaaatt ttacaaaaac tatctgctga aaaattgata tccggcggtg cgcctgctcc
      901 ggcggctaaa ccggcaaaag agaaagcgcc gtcgaagaaa gccgtcaaag aagacgcgcc
      961 taaagcgaaa gcggaaccga agccggaacc taaagcagaa cccaaaccgg agccgaaaat
     1021 tgaaaaagca ccggaaccca aacctgcgcc taaaaaagaa gaaaccgaca ttttgggttc
     1081 catggatacg cctaaggtcg aacccaagtc ggaacctaaa gtagaaaaag cggcgcccaa
     1141 accggtagct ccggccgctt ctgcgtcggc cggcggtcga aagatacttg tcgtggatga
     1201 cagtatcgtg attcaaaaaa tggttgagat tgcgttggaa aatgataatt acaatctgac
     1261 gtcggccatg aaaggcgaag atgcgatcaa caaagctaaa gaaatgcagc ccagtctgat
     1321 tcttttggat atgatgttgc ctgatatgac cggtgttgaa gtgatgaaag cgttgcgtga
     1381 aacgggcgga tattttgcca cggtgccgat cgtcgtgttg agcggtaaag acagtccgca
     1441 agataaagac gccgcgatca gcaacggcgc gacggatttc ctcaataaac cgttccatga
     1501 tgaagacttg cttgccaaag tacacgaata tctcggtaaa taaacgccat agcatcgcgt
     1561 aaatcgtaaa aaccctaaac gtgttttagg gtttttttgt atggatgata cacattggac
     1621 aataaatcgc gaaaaatcgt agaattttat ctttcggaat tactgaatca tgtcaaaagt
     1681 gtttccgaac atcgttttgc gccgcttgaa ttcagtatta ccaaaaaaat aatcgtatcc
     1741 atcggacaag cgcaagagcc ggttcgtcag gtcgaatcat ttctcaacgt ggcatcgctc
     1801 catccggttg ccaccttttt gcgtagtaac ttggagaaat tagaaaaatc cggctacaag
     1861 atgagccaga tgatcacgac gcttgaaaat gattccgagc ggatcattca gatgtttcaa
     1921 aaaattttag aaacacccgg ttcacaagat caactcaata acgatttaca aaccatcggc
     1981 ttttcgcttg atttgccggc cttacgaaaa acgtatatca agcaagaaga tacgccgtct
     2041 ccatcggaag gaacgcccaa ggatgaggtc aaaccgatat ccgttggcgt aaaaactgaa
     2101 cccccagctt caccggcgat aaaaaacgat tccatttttt ctcacaatac ctcccgtgca
     2161 gaatctaccc atgaaaaaac atcggtgacg tccgcatcac cgcaatcttc gctactggaa
     2221 gttgcgccta taacgcaatc cgaagcgttt gatcatgtgg gcgatatgat cggattcttg
     2281 tcacggcgaa caaaaaaaca acctgaaaaa gcggttgcac aaattgttca accaaccgaa
     2341 aaagttgacc cgaaagaaaa tataaatgca gtccggtcgg ttttttcaac ggaccgattg
     2401 aatagcgcat gggagacgtt gcgggataat ccgacacgcc ctcgatttct gattagcatg
     2461 gccgatggtc tgtcgcaact tagagcggag gccgtcacac aaaatgcaat gccattgtcc
     2521 gaggcgaccc agcggatcga agaaacgctg aatgcgattg cggataataa tcacaaacct
     2581 ctgaatccgg aggccttggc ggtgttgcac gatttgattc aacaggtcga aaacaacaat
     2641 tcagacttaa aatcgcttgc accggcagaa attttgaggc tacagcaacg gtttaccggt
     2701 tcgtttaata tggaaaaacc tgaagaatcg ccggttcagt cgtcaagttc aaatgcacag
     2761 cttgtagctg aaaatgaaat cgctattgat cgtgttgcga tacaagacga tgattggaat
     2821 gtatttgccg aagatgtgcg gtttaatttg gaattggcac agtcggcgtt ggtccgcctc
     2881 aaaaaagcta cgcacgacac cgaagccatc aaagaccttt tgcaggtata tcgtgcactg
     2941 tcaaccaata cgcggttatt aaaaatcaat ttactacacg ggcactgtga cgcggtcttc
     3001 cagattttca aacaagtagt ttattcgcaa agcccagtcg tcgcagaatt gatcaccctt
     3061 gtgacgcgat cgacgcgtag tatcgaaatt ttggtcaacg ggcaaacgat ccagtctgat
     3121 gaatggcgat ggctcaatga tcaatcggtt cgtttttcag attataaaca agcgacgaca
     3181 ttagtaacac ccgctaaaac gactgagata agcgccgtac catctgagca aacaaaagta
     3241 gttgcgccag aagttgtaaa aacggaaata aaaacggcga aagagtcttc aaaaccgata
     3301 tccgatccga agtccgtagt
//
LOCUS       DAOQNQ010000339         3319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_14265_length_3319_cov_5.498775, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000339 DAOQNQ010000000
VERSION     DAOQNQ010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14265_length_3319_cov_5.498775"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PK754_14465"
     CDS             complement(<1..219)
                     /locus_tag="PK754_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004819432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNB10753.1"
                     /translation="MLLYIALASGFLALAFAAYKAMWISKQEAGNDRMKEIATAIADG
                     AMAFLMREYKVLVIFVIVAGALLAVSGSM"
     gene            complement(295..870)
                     /gene="pth"
                     /locus_tag="PK754_14470"
     CDS             complement(295..870)
                     /gene="pth"
                     /locus_tag="PK754_14470"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNB10754.1"
                     /translation="MKLIVGLGNPGSRYESTRHNVGFMLIDKLARKHSVPMREGKGEY
                     FIGECMIGDERTFLMKPTTYMNNSGLAVRPFMAFFKIPVSDVLIACDDVVLPLGTIRM
                     RKEGSDGGQNGLKSVIYQLATDQIARLRIGVDHPLRCKMSLSDFVLSRFLEDEMAVLN
                     LVMDRATLAAESFVQHGVTRAMNQYNGGLPA"
     gene            complement(948..1598)
                     /locus_tag="PK754_14475"
     CDS             complement(948..1598)
                     /locus_tag="PK754_14475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNB10755.1"
                     /translation="MQETTLTAERRPAGKHSAKHARKQGKVPGVFYGYGQENVSIQLD
                     AMSLAKFLMTEHTLATITIDGKEHRVIVRDFDKDPVTGRLVHVDVMAVRMDRPIDVYV
                     PIVFIGSPIGVRTKGGIFQHDMTEFHIKCLPNDIPAHLEVKIDDLDLNHAVHIRDLNY
                     PNITILNPASESVCTVVIPKALESVLAEAQAAPTEPEVIAKGKEVAGEGEKAPEKK"
     gene            complement(1666..2595)
                     /locus_tag="PK754_14480"
     CDS             complement(1666..2595)
                     /locus_tag="PK754_14480"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNB10756.1"
                     /translation="MKYDIMLVSGRANRVFAEGIAKRLNIYLGKVEIVNFSDGEIFVK
                     YDENVRGKDLFIVQPTFPNSDNLMELLIMIDAAKRSSAARITAVIPYFGYARQDRKDQ
                     PRVPITAKLVANLLTTAGVDRILTMDLHSAQIQGFFDIPMDHLYSSAVLTEYWRGILK
                     KDAVIVSPDMGGVKLARAYAKRLELDFSIIDKRRPKANSVEIMNIIGHVEGRDVLLID
                     DMIDTAGTLTQAASALKEHGAQDIYAACTHPILSGNAIEKIMRSPIKKVVATDTIPMR
                     ASCEKIEILSVTHVFAEAISRIHKAESISVLFN"
     gene            complement(2666..2737)
                     /locus_tag="PK754_14485"
     tRNA            complement(2666..2737)
                     /locus_tag="PK754_14485"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2703..2705),aa:Gln,seq:ttg)
     gene            complement(2765..>3319)
                     /locus_tag="PK754_14490"
     CDS             complement(2765..>3319)
                     /locus_tag="PK754_14490"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10757.1"
                     /translation="LSRLLGLSLTDEELMHLGASLGSDVPFFVGFYLGHGATAKGQGR
                     GEVLSYFRWPLTEHVVVVYPNVHVSTPWAYQNFRTAIPSTVDTENERFNLTKRIESAI
                     LSAPLEKGLFFDNDFEYLVFREYPWIRTAQGVLVDLGAKVARMSGSGSTLYGLFDTVP
                     EFETLRERLGDAAIFRCRFIDSVK"
BASE COUNT          931 a          831 c          708 g          849 t
ORIGIN      
        1 catggatccg gaaaccgcca gtaaggcgcc ggcaacaatc acgaagatca caagcacttt
       61 gtattcgcgc atcaaaaaag ccatcgcacc atcggcaata gcggtggcaa tttctttcat
      121 gcggtcattg cccgcttctt gtttggatat ccacatcgct ttgtaagcag caaaggccaa
      181 agccaaaaag ccgctcgcca atgcgatgta gagcaacatt tgatcgttca cagggttttt
      241 ctcctcagat aatttttatt aaaacgaaaa tccgtattaa cagcctaaat tttactacgc
      301 cggtaaaccg ccgttgtatt gattcattgc ccgcgttacg ccatgttgga cgaaactctc
      361 agccgctaag gttgcgcgat ccatcacaag gttaagaacc gccatttcat cttccaaaaa
      421 tcgtgataaa acaaaatccg aaagactcat tttacagcgt aaaggatgat ctacgccgat
      481 ccgtagtcga gcaatctggt cggttgccaa ttggtaaata accgatttta aaccgttttg
      541 accgccatca ctaccttctt tacgcatgcg tatcgttccc aaaggcaaaa caacatcgtc
      601 acaggcgatc agcacatccg atacaggtat tttgaagaat gccataaaag gacgtacagc
      661 caaaccgctg ttgttcatat acgtcgtcgg cttcatcaga aacgtacgct cgtcaccgat
      721 catacattcg ccgataaaat actcgccttt gccttcgcgc attgggactg agtgtttgcg
      781 agcaagcttg tcaatgagca taaacccgac attgtgtcgg gtggattcgt aacgcgaacc
      841 gggatttcct aatcctacta ttagcttcat aatgtttcgg ccacacatac ggcaaatttg
      901 ttaatttgcc gtagtatgcc cgcaatgcag atttcagaaa ttccgaatta ctttttctcg
      961 ggtgcttttt caccctcgcc ggcaacttct ttacctttag cgataacttc aggttccgtc
     1021 ggagcggctt gtgcttctgc aagaacggat tcaagagctt tcggaataac aaccgtacaa
     1081 acggattcgg aagccgggtt gaggatagta atattgggat aattcagatc gcgaatgtga
     1141 accgcatggt tgaggtccaa atcgtcaatt ttcacttcaa ggtgagccgg gatatcattg
     1201 ggcagacatt tgatgtgaaa ttccgtcata tcgtgctgga aaataccgcc cttggtacga
     1261 acaccgatgg gcgaaccgat gaaaacgatc ggtacgtaca cgtcaatcgg acgatccata
     1321 cgaaccgcca tcacgtccac atgcactaaa cgaccggtta cgggatcttt gtcaaaatca
     1381 cgaacaatga cgcggtgttc cttgccatcg atcgtaatcg tggcgagcgt gtgttctgtc
     1441 atcaaaaatt tagcgagcga catcgcatca agctggatcg agacattttc ttggccgtag
     1501 ccataaaaaa cgccgggtac ttttccctgc ttgcgggcat gcttcgcact gtgctttccg
     1561 gccggacgcc gttccgctgt cagagtggtt tcttgcatgg tatcatcccc tgttttttag
     1621 gtgattatta gataatccgt acaaccgttg tacgaactga attttttaat taaacaaaac
     1681 gctgatggat tccgctttat gaattcgcga aatcgcttcg gcaaacacgt gtgttaccga
     1741 gagaatttca attttttcgc agcttgcccg catcggaatg gtatcggttg caacgacctt
     1801 tttgatgggc gaacgcatga ttttttcgat ggcgttaccg gaaaggatcg gatgcgtaca
     1861 agcggcatat atatcttgcg cgccgtgttc tttgagagcc gaagccgctt gagtcaatgt
     1921 ccccgcggtg tcaatcatat catcgataag caatacatca cgcccttcga cgtggccgat
     1981 gatattcatg atttcaaccg aattggcttt cggacgacgc ttatctatga tggaaaaatc
     2041 caattccaat cgtttggcat aagcgcgagc cagtttgacg ccgcccatat ccggtgaaac
     2101 aatgacagca tcttttttca agatcccgcg ccagtattcc gtcaataccg ccgaggaata
     2161 caagtgatcc atcggaatat caaaaaaacc ttgaatttgt gcgctatgca aatccatcgt
     2221 caaaatacga tccacaccgg ccgttgtaag cagattggca acgagcttcg ccgtgatcgg
     2281 cacccgaggc tgatctttac gatcttggcg cgcgtaacca aaatatggaa tgacagccgt
     2341 gatacgagcc gccgacgaac gtttggcggc atcaatcatg atcagaagtt ccatcaaatt
     2401 atcggaattg ggaaatgtag gctgaacaat aaaaagatct ttaccgcgca cgttttcatc
     2461 atactttaca aaaatctcac cgtcactgaa gttgacgatt tcgactttgc cgaggtagat
     2521 attgagtcgt ttggcgatac cttctgcaaa tacacgattg gcacggccgg agaccagcat
     2581 gatgtcgtac ttcatctcaa ccataacacg ccttttctac tgcttaaaaa tcaaaaagcc
     2641 cagagttccg ggcttatgta tcatgctggg gtgcaaggac tcgaacctcg gaatggatgg
     2701 accaaaacca tctgccttac cacttggcta caccccagtt ataaaagaac aaaatccaga
     2761 atttctactt taccgaatca ataaaacggc accgaaaaat cgccgcatca cctaagcgtt
     2821 cgcgcaacgt ttcaaactcc ggcacagtat caaaaagccc gtacaacgtt gagccgcttc
     2881 cggacatacg agccactttt gcgcctaaat caaccaaaac gccttgagca gtgcgtatcc
     2941 acggatactc gcgaaacact aaatattcaa aatcattatc aaaaaacagg cctttttcca
     3001 aaggcgcgga aagtatagcc gattcgatcc tttttgtcaa gttaaatctt tcattttcag
     3061 tatctaccgt agaaggtatc gcggttctaa aattttggta cgcccatgga gttgaaacat
     3121 gtacgttcgg ataaacaact accacatgtt ccgtcaacgg ccaacgaaaa tatgataata
     3181 cctcaccgcg cccttgtcct tttgcagttg cgccgtgacc aagataaaat cctacaaaaa
     3241 acggaacatc cgaacccaaa gacgcaccga gatgcattaa ttcctcatct gtcagcgata
     3301 accccaataa gcgtgagag
//
LOCUS       DAOQNQ010000340         3243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_14878_length_3243_cov_4.046110, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000340 DAOQNQ010000000
VERSION     DAOQNQ010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14878_length_3243_cov_4.046110"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PK754_14495"
     CDS             <1..390
                     /locus_tag="PK754_14495"
                     /inference="COORDINATES: protein motif:HMM:NF024490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HNB10758.1"
                     /translation="CLKPGVKGLSENIEVVSIIDRFLEHARIFYFHNNDQPEYWLASS
                     DWMPRNLDQRVEVMFPILEPRHQQTVMTVLQTQLGDNVKARRFSADGTYVRVKNDRKK
                     IRSQQVLYEIAGKLAKIYTVNESKIFK"
     gene            complement(357..>1315)
                     /gene="rsgA"
                     /locus_tag="PK754_14500"
     CDS             complement(357..>1315)
                     /gene="rsgA"
                     /locus_tag="PK754_14500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNB10759.1"
                     /translation="QDRDRRPESKPPVVQKNRVLGGYICGMNGSVYTVLAENKEYTCT
                     VQEYITRQGPLYIGDRVRIQIQPEMRGVIEKYEPRQNFVLAPGARERQGDLVLAANVN
                     QIVLVLSVMEPVLRTDWLDRHLLVCERKGFKPLIVCHKIDLADDNMFLEQMEVYKQMG
                     YRVIFTSSAVLSSVNELKAAVKQKTSLFTGHQGVGKTTLAELILEEESARLPDNPEEY
                     DLTIVDDIVETTRQVHCLRGKEGIVLIDTPGVTEYEISSIEKKDLKKYFRDFRNYNHQ
                     CSIPDCNHADEPNCKVKKAVETGEISEERYQNYLNILDSLTV"
     assembly_gap    1316..1415
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1416..1634)
                     /locus_tag="PK754_14505"
     CDS             complement(<1416..1634)
                     /locus_tag="PK754_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10760.1"
                     /translation="MSEPTNNDWSKKYLDDLNAKIKANKDVPKSATDHSPKEQIKPQV
                     TPKDQPRHQQQQRDQQNNNKPQRDNQRKE"
     gene            complement(1643..2140)
                     /locus_tag="PK754_14510"
     CDS             complement(1643..2140)
                     /locus_tag="PK754_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNB10761.1"
                     /translation="MEHLWAPWRMRYIKAGDKNEGEGCIFCFKPQEDRDRENMILFRS
                     THNFVIMNLYPYNNGHLMVVPYQHAPDINALPPETLLDMMTTTQKCVTAFQRSVKPDG
                     FNIGFNLGRTAGAGIDDHVHMHVVPRWNGDTNFMPVIMDTKVMPEYLEQTYDRLLAEF
                     SQMKK"
     gene            complement(2167..>3243)
                     /locus_tag="PK754_14515"
     CDS             complement(2167..>3243)
                     /locus_tag="PK754_14515"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF016438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNB10762.1"
                     /translation="LNAGGVHHVRVALPLRSDLHDTMQAVNEIKQLSAHQTDNPFKAE
                     ILYAPVRNYQDITAWKRMREAIEEDLLTVALIADCSHSTDMACAVLPFTDGVRWTPDK
                     AWTADVYEAHVRKLIESIAGFEKKILQIHFSPTEIPSFVDRHVQSSSEKTGWMAIRVA
                     QIAHEAGFSNLMISIQDSDFIAAHRILIAQLNAAGFNTPMVLHFEHKANPLFDAASHI
                     GSLLSDGIGDVIYLDCRAMASEETVRLAYNILQAARLRVSKTEYISCPSCGRTLFDLQ
                     EVTARIKSKTGHLKGVKIAIMGCIVNGPGEMADADFGYVGASAGKINLYIGKELVERN
                     IDMADADQKLIDLIRREGRWIEPA"
BASE COUNT          830 a          765 c          669 g          879 t
ORIGIN      
        1 tgtttgaaac ccggcgtaaa aggattgtcg gaaaacattg aagtagtcag catcattgat
       61 cgttttcttg agcacgcgcg tattttttat tttcataata acgaccaacc ggaatattgg
      121 ctcgccagtt cggactggat gccgcgtaac ttggaccaac gcgtcgaagt catgtttcct
      181 atactggaac cgcgccatca gcaaacagtg atgaccgtac tccaaacgca gctcggcgat
      241 aatgtaaaag cccgccggtt ttcagccgac gggacttatg tacgagtcaa aaatgatcgt
      301 aaaaaaattc gttcgcagca ggtactgtac gaaatagctg gtaagttagc caaaatttac
      361 accgtcaacg aatccaaaat attcaaataa ttttgatagc gctcttctga aatctctccg
      421 gtttcaacag cctttttgac tttacaattc ggttcatccg catgattaca atcgggaata
      481 gaacattggt gattgtaatt tcggaaatca cggaagtatt ttttaagatc ttttttctca
      541 atactcgaaa tctcatattc ggtaacgccc ggcgtatcaa tgaggacaat tccttccttc
      601 ccgcgcagac aatgtacttg acgggtggtt tccacaatat catccacgat cgtaaggtca
      661 tactcttcgg gattatccgg caatcgagcg ctttcttctt cgagtataag ctccgccagc
      721 gtagttttcc cgactccttg atgaccggta aataacgaag tcttttgttt tacggcagct
      781 tttagctcat tcacactgga gagcacagcg gacgaggtaa atataacgcg atatcccatc
      841 tgtttataga cttccatctg ctctagaaac atattatcat cggccaaatc aattttgtgg
      901 catacgatca gtggtttgaa acctttacgc tcgcacacca gcaaatgccg atccagccaa
      961 tccgtacgca acaccggttc cattaccgac aggactaaaa cgatttgatt gacattagct
     1021 gccagtacca aatcaccttg gcgttcgcgt gcaccgggag ctaacacaaa attttgacgc
     1081 ggttcatatt tctcgatcac gccgcgcatc tcaggctgga tttgaattcg gacacgatcg
     1141 ccgatatata acggaccttg acgtgtgata tactcctgaa ccgtacatgt atattcttta
     1201 ttttccgcta aaacagtata cactgagccg ttcattccgc agatatatcc gccaagaact
     1261 cggtttttct gtacaaccgg cggtttagat tccggtctgc gatcgcgatc ttgggnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctctt tgcgttgatt atcgcgttgc
     1441 ggcttattgt tattctgctg atcgcgctgc tgttgctgat ggcgcggttg atctttagga
     1501 gtaacctgag gttttatttg ctctttcggg ctatgatccg tagccgattt gggtacatct
     1561 ttgttggcct taatcttggc attaagatca tccagatatt ttttggacca atcattgttc
     1621 gtcggttcag acataaaact ccttacttct tcatctgcga aaattcagcc aacagacggt
     1681 catacgtctg ctctaagtat tcaggcatca ctttggtgtc catgattacc ggcataaaat
     1741 tggtatcgcc gttccatcgc ggcacgacgt gcatgtggac gtggtcgtca atgcccgctc
     1801 cggcagttcg tccgagatta aatccgatat tgaaaccatc cggtttgacg ctgcgctgaa
     1861 atgctgtgac gcatttttgc gtggtcgtca tcatgtccaa tagcgtttcg ggcggcaaag
     1921 cattgatgtc cggagcatgt tggtaaggca ccaccatcaa atgaccgttg ttgtacggat
     1981 ataagttcat tattacaaaa ttatgcgtcg agcggaaaag gatcatgttt tcacggtccc
     2041 gatcttcttg cggtttaaaa caaaagatac atccttcgcc ttcatttttg tcgccggctt
     2101 tgatataccg catgcgccag ggtgcccata aatgttccac tacgttactc catcctaagt
     2161 ataaaattat gccggttcga tccatcgccc ttcgcggcga ataagatcaa taagtttttg
     2221 atcggcatcg gccatatcaa tattacgctc gactaattct tttccaatat acagattgat
     2281 cttcccggcg ctggctccga catatccgaa atcagcatcg gccatttcgc ccggtccgtt
     2341 gacgatacaa cccataatag cgattttaac gcctttcaaa tgaccggtct tggatttgat
     2401 acgtgcagta acttcctgca aatcaaacaa tgttcggccg cacgaggggc acgaaatgta
     2461 ttccgttttg gaaacacgta agcgagccgc ttgcaaaatg ttatacgcta agcgcacggt
     2521 ttcttccgac gccatagccc ggcaatctaa ataaataaca tcgccaatac catcggaaag
     2581 cagagaaccg atgtgcgaag cggcatcaaa taagggattc gctttatgtt caaaatgcaa
     2641 aaccatcggc gtattaaaac cggctgcatt cagctgagcg atcagtattc gatgcgcagc
     2701 aataaaatca ctatcctgaa ttgaaatcat caaattacta aatcccgctt cgtgcgctat
     2761 ctgagcaacg cggatcgcca tccaacctgt tttctccgat gatgattgga catgacgatc
     2821 aacgaaagaa ggtatctcag tcggtgaaaa atgaatctgc aaaatttttt tctcgaatcc
     2881 ggctattgat tcgataagtt tacgaacatg cgcttcgtac acatcggccg tccacgcctt
     2941 atccggagtc caacgtacgc catcagtaaa aggaagtacg gcgcaagcca tatctgtcga
     3001 atgactacaa tccgcaatta aagcaaccgt taataaatcc tcttcaatcg cttcacgcat
     3061 gcgtttccat gccgtgatat cctgataatt gcgtaccggt gcgtacaaaa tctcggcttt
     3121 aaagggatta tcggtctggt gagcggaaag ctgtttgatt tcatttacag cctgcatcgt
     3181 atcatggaga tcactccgca gcgggagagc aacacgcaca tgatgcacac cgccggcatt
     3241 aag
//
LOCUS       DAOQNQ010000341         3231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_14979_length_3231_cov_5.608627, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000341 DAOQNQ010000000
VERSION     DAOQNQ010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14979_length_3231_cov_5.608627"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..878
                     /locus_tag="PK754_14520"
     CDS             153..878
                     /locus_tag="PK754_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNB10763.1"
                     /translation="MPRHIAIIMDGNGRWAKQRGLPRIAGHNEGVNSVKDITEGCGEL
                     GIEALTLYAFSQENWKRPSWEVSALMKLLMRTILNQIDSLNRQNVRVKTIGHIDMLPT
                     GTLNQVEKAIEITKNNTGLVLNLALSYSGRIEILDAVKRLYQDAARKKSLMDSLDESV
                     FAKYLDTFDLPDPDLVIRTSGEFRVSNFLLWQIAYAELYITDIYWPDFRKPQLYKAIL
                     DYQKRERRFGKVSEQIAEEKKEK"
     gene            944..>3231
                     /gene="bamA"
                     /locus_tag="PK754_14525"
     CDS             944..>3231
                     /gene="bamA"
                     /locus_tag="PK754_14525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNB10764.1"
                     /translation="MKLAKIIVITAFLLCLGSNRASALFAQSGAKPKIASIKIEGNIK
                     SDPELIVIATGLSVDQEFNLDDIQKAMENLWQMNVFKDIQVFGEQTENGIAVVFEVKE
                     YPRLENLEINGTDEIEEKDVRSKFGLFNGQTVSPQHIKKATERVRQLYASEGYRNVEI
                     DVKSYNSETDTGKVLLKIEIEENDKVKIRGITFHGNTAFSNGKLEGAFDDTKAKRGFW
                     KWFKSGDFDEKKYNEDLKKLLMFYKKRGFRDIQIMKDSVYYAQNNKDLYIDIWVEEGV
                     KYHIGNISFTGNTLFSGDELTAALGFERGDVYDQEKYDRNMQERVNALYYDRGYIYAQ
                     LVPIERPSNKDTLNLEFVVTEGNQVDIERVEIRDNTKTKEKVIRRETVAYPGEKFSRD
                     ALIRTQRNLMVLNYFETVIPDVQPVSQDKVNVIITVKEKPTDTANLSIGYSAQDGLIG
                     SAGVAFNNFLGNGQVVNLNLQLGGAGYKVFSIGFQEPYLFDTRTSFGASMYYSIDGNR
                     RAQYVGYKSQSYGGSVNFGRRLKWPDDFFTASWSVAYGNSKLKPLSTSQVSDYIAYGH
                     TQSVTLSQVINRDSRDAAEFPKTGSVFTLTSDIGFFSIDTVGYLNSLRVLPKNFFKNT
                     FRAQNYMPLFWNFVFYTDFTMGYVRTFQSTSTVEDIPQFDRFYMGGGALDIGSIQMRG
                     YGGRKVGGQAVTESGISYPIGGTSMVKYSAEIRLPVIPNPTMFILGFAEAGNVFQSLK
                     TTDPFQVKRSIGYGFRLFMPLVG"
BASE COUNT          957 a          610 c          759 g          905 t
ORIGIN      
        1 atcttttatt gatctacgga gcgtttcaag gcgtggcctt attgaagtca tcttcagcga
       61 attccgaatt tgatatactc aaaatcgtcg caaagaaaaa actctcagaa tcagatcatg
      121 cattggtacg tgctgaattg caaaaacggt ctatgcctcg ccatatagcc attattatgg
      181 atggtaacgg ccgctgggct aaacaacgtg gattaccgcg gattgccggg cacaacgaag
      241 gcgttaattc ggtcaaagat atcacggagg gttgtggtga actgggcatc gaagcgctga
      301 cactgtatgc tttcagccag gaaaattgga aacgcccaag ctgggaggtt tcggccttga
      361 tgaagctgct tatgcgtacg attctcaatc agattgatag ccttaatcgt caaaatgtac
      421 gagttaaaac aatcggccac atagatatgt tgcccacagg tacgctaaat caggtagaaa
      481 aagcgattga aattactaaa aataatacag gcttggtgct caatctcgct ttgagttata
      541 gcggtcgtat tgaaatactc gacgctgtaa aacggcttta tcaagatgct gcacgaaaaa
      601 aatcattgat ggattcgctg gacgaatcag tatttgctaa atacctggat acctttgatc
      661 tgccggatcc ggatcttgta atacgaacga gcggagaatt tcgtgtaagt aactttttac
      721 tttggcagat tgcgtatgcc gaattgtata taacggatat ttattggccg gattttcgca
      781 aacctcagct gtacaaggcc atattggatt atcaaaaacg cgaacgacgt tttggaaaag
      841 taagcgagca gatcgcggaa gaaaaaaaag aaaaataata acattttatc gtatacccac
      901 gtaactcagt atgtatcaat atccggagac aacgaatcac gtaatgaaac ttgcaaaaat
      961 catcgtcatc accgcctttt tgctctgctt aggaagtaac cgtgcatccg ctttgtttgc
     1021 gcagtccgga gccaagccta aaatcgcatc cattaaaatc gaaggcaata tcaagtccga
     1081 tccggaattg atcgttattg ccacaggtct ttcggtagat caagagttta atctggatga
     1141 tattcagaaa gccatggaaa atctatggca aatgaatgtc ttcaaagata ttcaggtttt
     1201 tggcgaacaa acggaaaacg gaattgccgt cgtatttgaa gttaaagaat acccgcgttt
     1261 ggaaaatctt gaaataaacg gaacggatga aattgaagaa aaagatgtac gttccaaatt
     1321 tggtttattc aatggtcaga cggtaagccc gcaacatatc aaaaaagcta ccgaacgcgt
     1381 gcgccagctt tatgcgagcg aagggtatcg caatgttgaa attgacgtga aatcttataa
     1441 ttcggaaacg gataccggca aggttttact taaaatcgaa attgaagaaa acgataaagt
     1501 gaagattcga ggaataactt ttcacgggaa tacggctttt agcaatggta aattggaagg
     1561 ggcctttgac gacactaaag ccaagcgcgg attttggaaa tggtttaaga gcggtgattt
     1621 tgatgaaaag aagtataacg aagaccttaa gaagttgctg atgttttaca agaagagagg
     1681 ttttcgtgat atccaaatta tgaaagattc agtgtattac gctcagaaca acaaggacct
     1741 gtatattgat atttgggtcg aagaaggtgt taagtatcac atcggtaata tttcgtttac
     1801 cggcaataca cttttttcag gtgacgaact tacggcagca ttgggctttg aacgcggtga
     1861 tgtgtatgac caagaaaaat atgaccgtaa catgcaggaa cgagtaaatg ccttgtatta
     1921 tgataggggt tatatttacg ctcaacttgt tccgattgag cgtccatcta ataaggatac
     1981 actcaatctc gaatttgttg ttactgaagg caatcaagtt gatattgagc gtgtggagat
     2041 acgcgataat accaagacaa aagaaaaagt aattcgccgt gaaacggtgg cctatccggg
     2101 tgaaaaattc agtcgtgatg cgttgattcg tacgcagcgt aacttgatgg tgttgaatta
     2161 ttttgaaacg gttatacctg acgtacaacc ggtgagccag gacaaagtaa atgttatcat
     2221 cacggtaaaa gagaagccga cagatacagc caatctttca ataggctata gcgctcagga
     2281 cgggttgatc ggttctgccg gtgtggcatt caataatttt ttgggcaacg gtcaggtcgt
     2341 aaacctcaac ttacagttag gcggtgccgg ctataaagtg ttttccatag gatttcagga
     2401 gccctattta tttgatactc gcacttcgtt cggcgccagt atgtattact cgattgatgg
     2461 taatcgccgt gcacagtatg tcggctataa gagccaaagt tatggcggct ccgttaactt
     2521 cggtcgtcgc ctcaagtggc ccgacgattt ttttacggcg agctggtctg ttgcttatgg
     2581 taattcaaaa cttaagcctt tgagtacgag tcaagtgtca gactatattg cgtacggcca
     2641 tactcaatct gtgacgttat cgcaggtaat aaatcgtgac agccgcgatg ccgctgaatt
     2701 tccaaaaacc ggttcggttt tcacattgac gtctgatatc ggtttctttt ctattgatac
     2761 tgtcggctat ttgaattcgc tgcgcgtgtt accgaaaaat ttcttcaaaa acacattccg
     2821 tgctcagaat tatatgccgt tattctggaa ttttgtcttt tatacggact ttacgatggg
     2881 ttatgttcgt acatttcaat ccacttcgac tgtggaggac attccgcaat ttgatcgctt
     2941 ctacatgggc ggcggtgcgt tggatatagg gtcaatccaa atgcgcggat acggcggaag
     3001 aaaagtcggc ggtcaagcgg tgactgaaag cggcatttcg tatccgatcg gaggaacgtc
     3061 aatggtaaaa tattccgctg aaattcgttt gccagttatt ccgaatccaa cgatgtttat
     3121 tttaggattt gcagaagcgg gcaatgtatt tcagtcttta aagacgacag atccatttca
     3181 ggtcaaacgt tctatcggtt atggtttccg cttatttatg ccgcttgtag g
//
LOCUS       DAOQNQ010000342         3224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_15030_length_3224_cov_5.545914, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000342 DAOQNQ010000000
VERSION     DAOQNQ010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15030_length_3224_cov_5.545914"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PK754_14530"
     CDS             complement(<1..242)
                     /locus_tag="PK754_14530"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNB10765.1"
                     /translation="MIKKFMVPEDKKISLKDYDPDETLGYENDDSVKEEFKKLNLKMI
                     DLQELLYAEKEKSLLIILQAMDAGGKDGTIKHVMRGL"
     gene            342..2750
                     /gene="pheT"
                     /locus_tag="PK754_14535"
     CDS             342..2750
                     /gene="pheT"
                     /locus_tag="PK754_14535"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNB10766.1"
                     /translation="MKISLTWIKDYVDIKLSPQELAHRLTMNGLNVEAILENDPFKGV
                     VLGKVLEVTKHPNADKLSLTKVDVGTDILNIVCGAPNVKAGQLAPIATIGTMMPGNFE
                     IKKAKIRGEESCGMICSKSELGFETGKSPGIWELDSTTPHTLGQSFAAFIGSGEVVLD
                     IDVTSNRPDCLHMLGMAREIAMIENTTIRMPDVRVIESSEKAEAHASIAIEDREGCTR
                     YAGRVIRNVTIGPSPDWLVKRLEAVGIRSINNIVDITNFVMLECGQPLHAFDYDHLAG
                     HKIIVRKSRKGDTFQTLDDKSHTLNDETVMICDGEKMIAIGGIMGGKNSEISDSTKNV
                     LLESAHFDGKRIRRSSKHLGIMSEASNRFGRGINPEGVIYAIQRAAQLMHELAGGEIL
                     AGVLDVNFSITKPVVITLRSASVTRLIGQEIPDNDIADILTRLGFGVINNGNGSFIVT
                     APSHRVDIEIEENLIDEVARIYGFDRIRPSTGAYGHYHHEPPSGEKMMHQTRAALREA
                     GLSEVYTNAMVHPSSQLAVEPEGEKYFVRLMNPISEDMSVMRISMLPSLLEVVRGNLF
                     RKNDSMQIYEIGRTYRNEGDPLPAERNVLAGCIVGQSAPTHWATKPRAIDYYDLKGIV
                     DRIMGKFWLDSLQYNPYHSFVFAPNSVSISCLASDSQVLTLGRFGRIQNAVLRALDID
                     TTVWAFEIDYDVLRRVGKFRKTFDLISRFPFIRRDLALIVDEKMQAGAIQNAIRSKAG
                     AHLTNLEVFDVYHGNQVTQGQKSLAFALRFESVERTLTEDEIDATMQSIIAYVENQFG
                     AKLR"
     gene            2766..3212
                     /locus_tag="PK754_14540"
     CDS             2766..3212
                     /locus_tag="PK754_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10767.1"
                     /translation="MARASEKHSRTRSAGSLDLVEIRLKKLLQDLHDAYAEIGRLQEI
                     TQQADHQVTEAVAEKQRELDDKEKIVRRQQNLIKTKDAAYQELQDAYNALRREADELK
                     RGSESPAQTVEIRSEPKAMLDPEFRTRMRVQLESVLNKINELEKII"
BASE COUNT          867 a          793 c          773 g          791 t
ORIGIN      
        1 agtccgcgca tcacgtgttt gatcgtacca tctttaccgc cggcatccat ggcttgcaat
       61 atgatcagaa gtgatttttc tttttcggca tataagagtt cttgcagatc aatcatcttc
      121 agattgagct ttttaaactc ttccttgacg gaatcatcgt tttcgtaacc gagggtttca
      181 tccggatcat agtccttgag tgaaattttt ttatcttcgg gcaccataaa tttcttgatc
      241 atgacacgct cctcaggtta aaaacccttg attaaaatac atttaggtca tattcttgca
      301 ttacgcaaat atacgatcac gtttcaatat acggagtttt catgaagatt tccctgactt
      361 ggatcaagga ttatgttgat atcaagttgt ccccgcaaga gctggcccat cgtcttacga
      421 tgaatggact taatgtcgaa gccattttgg aaaatgatcc tttcaaaggt gtggtcctcg
      481 gtaaagtgct cgaagtaacc aagcatccca atgccgataa actgtcgctc accaaggtag
      541 atgtcggcac ggacatactg aacatcgtct gcggagcgcc caacgtcaaa gccggccaat
      601 tggcccccat cgctacgatc ggcacgatga tgccgggaaa ttttgaaatc aaaaaagcaa
      661 aaattcgcgg cgaagaatcc tgcggtatga tttgctccaa aagcgaattg ggatttgaaa
      721 ccggcaagtc gcccggcatt tgggaacttg attctacaac gccgcatacc ttaggtcaat
      781 cgtttgctgc gtttatcgga agcggcgaag tcgtactgga tattgatgtg accagcaacc
      841 gtcccgattg cctccatatg ctcggcatgg cacgcgagat cgccatgatc gaaaatacga
      901 ccatccgtat gcccgacgtg cgtgtcatcg aatcctcgga aaaagccgaa gcgcatgcgt
      961 ctatcgctat cgaagatcgc gaaggctgca cacgctatgc cggacgcgtc atacgcaacg
     1021 tcacgatcgg cccctcgccg gattggttgg tcaaacgact cgaagccgta ggtattcgtt
     1081 cgatcaacaa catcgtggac attaccaatt ttgtcatgct ggaatgcggc caaccgctgc
     1141 acgctttcga ctacgatcat ctggccggcc acaaaatcat cgtgcgtaaa tcccgcaaag
     1201 gcgatacgtt tcaaacgctg gatgataagt cgcacacact caatgatgaa acagtgatga
     1261 tctgcgacgg cgaaaaaatg atcgctatcg gcggtatcat gggcggaaaa aattcggaaa
     1321 tttcggattc gaccaaaaat gttctcctcg aatcggccca ttttgacgga aaacgaattc
     1381 gccgctcatc caaacacctc ggtatcatgt cggaagcatc caatcgtttt ggccggggca
     1441 tcaaccccga aggcgtgatc tacgccatcc agcgcgccgc acaactgatg catgaactgg
     1501 ccgggggcga aattctcgcc ggtgtattgg atgttaactt ttcaatcacc aaaccggtgg
     1561 tgatcacatt gcgctcggca tcggttacgc gccttatcgg tcaagaaatc cccgacaacg
     1621 atatcgcgga tattcttacg cgccttggct ttggcgtcat caataacggc aacggttcat
     1681 ttatcgtcac cgcgccatcg catcgcgtag atatcgaaat cgaggaaaat ctgattgatg
     1741 aagtggcccg catttacggt tttgatcgca tccgtcccag taccggcgcc tacggacact
     1801 atcatcacga accgccttcc ggcgaaaaaa tgatgcacca aacccgcgcc gctttacgtg
     1861 aagccggttt atcggaagta tataccaatg ccatggtaca tccttcatcg cagctggccg
     1921 tcgaaccgga gggtgaaaaa tattttgtgc gcctgatgaa ccccatcagc gaagacatgt
     1981 ccgtcatgcg catttccatg ctgccgagtt tgctggaagt cgtacgcggt aatctatttc
     2041 gtaaaaacga cagcatgcag atctatgaaa tcgggcggac gtaccgcaac gagggcgatc
     2101 cactgccggc ggaacgtaac gtattggccg gttgtatcgt cggccaaagc gcccctaccc
     2161 actgggcaac caaaccccgt gccatagatt attatgatct taagggcatt gtcgatcgga
     2221 ttatggggaa gttttggctt gattcgctac agtataatcc ttatcattct ttcgtatttg
     2281 cgcccaattc ggtttccata tcctgtcttg cttcggattc tcaggtatta acacttggtc
     2341 gctttggacg tatacagaat gcggtcttac gggcattgga tattgacacg accgtttggg
     2401 ctttcgaaat agattacgat gttttacgac gtgtcggaaa attcagaaaa acatttgatc
     2461 tgatttccag atttccattt attcgccgcg acttggcctt gatcgtggat gaaaaaatgc
     2521 aggccggcgc gatacagaat gccatacggt ccaaggccgg tgcgcatttg accaacttgg
     2581 aagtatttga tgtgtatcac ggcaatcagg taacacaagg tcaaaaaagc ctcgcgtttg
     2641 cgttgcgatt cgaatccgtc gaacgtactc tgacggagga tgagatcgat gcgaccatgc
     2701 aatccatcat cgcgtatgtc gaaaatcaat ttggcgcaaa actgcgataa caggtaataa
     2761 caaaattggc gcgagcatcc gaaaaacatt cacgtacacg cagtgccggc tcccttgatt
     2821 tggtggaaat ccggttaaaa aaattgctac aggatctgca tgatgcgtat gcggagatcg
     2881 gacgtttgca ggagatcacg cagcaagcag accatcaggt gacggaagcc gttgccgaaa
     2941 aacagcgtga acttgacgac aaagaaaaaa tcgtccggcg gcaacaaaac ctgatcaaga
     3001 ccaaagatgc ggcgtatcag gaattgcagg atgcgtataa cgcccttcgc cgtgaggccg
     3061 atgaactaaa acgcggcagt gagtccccgg cacagaccgt ggaaatacgt tctgaaccta
     3121 aagcgatgct ggatcccgaa ttcagaacgc gcatgcgggt gcaattagaa agtgttttga
     3181 ataaaataaa cgaactggaa aaaattattt aattttttgc gata
//
LOCUS       DAOQNQ010000343         3197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_15265_length_3197_cov_4.263845, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000343 DAOQNQ010000000
VERSION     DAOQNQ010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15265_length_3197_cov_4.263845"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PK754_14545"
     CDS             <1..352
                     /locus_tag="PK754_14545"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB10768.1"
                     /translation="YIIDEIDNSLHPHLCYEFVKLLMSGRAENSHNNFIDFTKRKKNS
                     QIIFTTHQPNLLTRELFRDDEIWFVEKDKHKGNTSLYPLFEFGVKADFNIEDGYLLGR
                     FGGVPLIDTHTYMQ"
     gene            359..1069
                     /locus_tag="PK754_14550"
     CDS             359..1069
                     /locus_tag="PK754_14550"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HNB10769.1"
                     /translation="MAKKRKYTKEEVRDQRRSYSMIWIYPEGSEVEVDYFEYFKDISS
                     RIKVVINNDADKEKKDTRSANKWLIDRYAEIRNNIDKTIGDSAWFIIDVDRKSNEDIH
                     NLDTAVRDDGIAKVIISNPCFEVWKLYHCNHSEPYNTSCQIMKTRLHEITKGKSHPLN
                     LLSGIYDAIKLAKSEDPQPGYFFPKPDVVCYSKVYELIEYIQEKITVDEFNNFIKYRI
                     PEKLDAYNRNITERKSGG"
     gene            complement(1196..2680)
                     /locus_tag="PK754_14555"
     CDS             complement(1196..2680)
                     /locus_tag="PK754_14555"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB10770.1"
                     /translation="MGTFDRDDKDGWREREGDEEEPWKKAHENWKNDSRLSDDVPATR
                     ETSPFWTEERWEHFIQETDKDGLQVIKYYERYWNHQDRDVMVENAMALHYVREAFKYI
                     HPENHKSFYVHYFLEPECALMKLPVQSSGHFLEAKLRTVSDIQAYRLSRDFARFAYRW
                     LKDLPDEFGVAYSRNITQHPTKGIGNWTDGELLWLLRTGIHPKTGKYLPPWMPKFIRM
                     SDEDIHSIIAFLRSDDPLVKSADVDNIESQPSFLAKMLARIAFKPFEYPSAPIAPPDT
                     NNPIEHGKYLANGVFDCYACHSGDFKTMNVFTPEQSGDYYAGGNKMLDVNRLLVPSVN
                     ITPDKETGIGKWTEQDFMKAMKTGFRPDGTLLRYPMGRFHRLSDTEIRHIYAFLRTVP
                     PIQKQNMKAETMQPSANASSGEKHYISYGCVGCHGATGLGYADLQQAHKKYPSDSLLA
                     DVIMHPTTYFGALTTMPTWEGHLQQQHLPDLVAYVRELGKKSGR"
     gene            complement(2684..3196)
                     /locus_tag="PK754_14560"
     CDS             complement(2684..3196)
                     /locus_tag="PK754_14560"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNB10771.1"
                     /translation="MDGHLLKAWRDVVQLPDGKTGIREYIRHPGAVIMLPLFENGDTM
                     LIRQFRFPPKKIFWELPAGKLDGPEDPLVAAQRELIEETGWSASHWTRVGSFYPCIGY
                     SDERMILYIAEDLTPAESAHDDDEFIERFRMPRGDALQKVWSGEIDDMKTIAALLLAE
                     HTLRNNSKTR"
BASE COUNT          927 a          643 c          699 g          928 t
ORIGIN      
        1 atatatcatt gatgaaatag ataattcact tcatccacac ttatgttatg aatttgtgaa
       61 attacttatg tcaggcagag cagaaaattc gcataataat ttcatagatt ttacaaagag
      121 gaagaaaaat tcgcagatta tttttactac gcatcaacca aatttactga ccagagaact
      181 atttcgcgat gatgagatat ggtttgttga aaaagacaag cataaaggta atacatcatt
      241 atatcctttg tttgaatttg gtgtgaaagc agattttaat atagaggatg gttatttgtt
      301 gggtcgtttt ggaggcgtac cgcttattga tacacatacg tatatgcaat aagaagctat
      361 ggcaaagaaa cgtaaatata caaaggaaga agttcgagat caaagacgca gttattcgat
      421 gatatggata tatccggaag gttcggaagt tgaggttgat tactttgaat attttaagga
      481 catttccagt cgaataaaag tagttatcaa caatgacgca gataaagaga agaaggatac
      541 tcgctctgca aataaatggc ttattgaccg atatgcagaa atacgaaata acatagacaa
      601 aacaataggt gacagtgctt ggtttattat tgatgtagat cggaaatcaa atgaagatat
      661 tcataattta gatactgctg tcagagatga tggaatagct aaagtaatta taagcaatcc
      721 ttgttttgag gtgtggaaac tgtatcattg taatcatagt gagccatata atacatcttg
      781 ccaaataatg aagactcgtt tacatgaaat tactaaaggt aaaagtcatc cattaaattt
      841 attatccggc atatatgatg caataaagtt agcaaagagc gaagaccccc aacccggata
      901 ttttttcccg aaacctgacg ttgtttgtta ttcaaaagtt tatgagctta ttgagtatat
      961 acaagaaaaa atcacagttg atgaatttaa taattttata aaatatagaa ttcctgagaa
     1021 attagacgct tataatcgta atattaccga gagaaagtcc ggcggctaaa gcattacaac
     1081 aattattgtg aaagaatgtg aaggaagtgc aaaaaaaaat gctacacaag aaacgtacag
     1141 tcttttgtgt agcattgttg ttttttgata tgccgtaagt acggcgtgat gtgcatcagc
     1201 gaccggattt tttccctaat tccctcacat aagcgacaag gtcaggcaga tgctgctgct
     1261 gaaggtgtcc ttcccatgtt ggcatagtgg ttaatgctcc gaaataggtt gtcgggtgca
     1321 taatcacatc agccaaaaga ctatcggaag gatatttttt gtgcgcttgc tgtaagtctg
     1381 catatcccaa gccggtagca ccatgacatc cgacacatcc atagctgata tagtgttttt
     1441 caccggatga agcatttgcc gacggctgca ttgtttccgc tttcatgttt tgtttttgta
     1501 tgggcggcac tgtgcgcaaa aatgcgtaga tatgacgtat ttctgtgtca gagagccgat
     1561 ggaatctgcc catgggatag cgtaataaag tgccgtcggg tcgaaatccc gttttcatgg
     1621 ctttcataaa atcttgttca gtccatttac cgatacctgt ttctttatcg ggagtaatat
     1681 tcaccgaggg aacgagcaat ctgttgacat ctaacatttt atttccgccg gcataataat
     1741 caccggattg ttcgggagtg aagacattca tggttttgaa atcacctgaa tgacaagcat
     1801 aacaatcgaa gaccccattt gccagatatt tcccgtgttc gatggggtta ttggtgtccg
     1861 gtggcgcaat gggcgcactc ggatactcaa agggtttgaa tgcaatgcgc gccaacattt
     1921 ttgccagaaa tgatggttgc gattcgatat tgtccacatc cgcggatttg acaagcgggt
     1981 catcactgcg taaaaatgca atgattgagt gaatgtcttc atcgctcatg cgaataaatt
     2041 tgggcatcca cgggggcaaa tatttccctg tttttggatg aatgcctgtg cgtaaaagcc
     2101 ataataattc accatcagtc cagttgccta tgccttttgt gggatgttgg gtaatattac
     2161 gagaatatgc cacgccaaat tcatccggca gatcttttag ccaacggtat gcaaaccgcg
     2221 cgaaatcacg cgataatcgg tacgcctgaa tatcagacac cgttcgtaac ttcgcttcaa
     2281 gaaaatggcc gctgctttgc accggcagtt tcatcaaagc acattccggt tctaaaaaat
     2341 aatgcacgta aaaagattta tgattttccg gatgaatata tttgaacgct tcccgtacgt
     2401 aatgaagcgc catggcgttt tcgaccatga catcacgatc ctgatgattc cagtaccgtt
     2461 cgtagtactt gataacctga agcccgtctt tgtctgtttc ctgaataaaa tgttcccagc
     2521 gttcttctgt ccaaaacgga gaggtttcgc gggtagccgg aacatcatcg gataagcggg
     2581 aatcgttttt ccagttttcg tgtgcttttt tccaaggttc ttcttcatca ccttcccgtt
     2641 cgcgccaacc gtctttgtca tcgcggtcaa atgtacccat acatcacctc gttttagagt
     2701 tgttgcgtag cgtatgttcg gccagcaaaa gcgcggctat ggttttcata tcatctattt
     2761 ccccggacca tactttttgc aatgcatcgc cgcgcggcat acgaaatcgt tcgataaatt
     2821 cgtcgtcatc gtgcgcggac tccgccggtg tgaggtcttc ggcgatgtac aagatcattc
     2881 gttcatcgct gtatccgatg cagggataaa acgaaccgac gcgcgtccag tgcgaagccg
     2941 accatcctgt ttcttcgatc agttcgcgct gcgctgccac cagcggatct tccggtccgt
     3001 ctaatttacc ggccggcaac tcccaaaata ttttttttgg aggaaatcga aattgacgaa
     3061 taagcatggt atcaccattt tcaaacaacg gcaacatgat caccgccccg gggtggcgaa
     3121 tatattcgcg aatgcctgtt ttaccgtcgg gcaactgtac gacatcgcgc caggccttga
     3181 gcagatggcc gtccacg
//
LOCUS       DAOQNQ010000344         3194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_15302_length_3194_cov_4.240204, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000344 DAOQNQ010000000
VERSION     DAOQNQ010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15302_length_3194_cov_4.240204"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PK754_14565"
     CDS             <1..398
                     /locus_tag="PK754_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10772.1"
                     /translation="GVDKTSLQGYSYQGMRWEDPADSLRILSGTNTGDIRKWNREEFF
                     LLRHHVKFLATDKAGPQIYNLQSDPYEEHNLWDDLSFREAHRPWIHQYISDHAVLAEL
                     AKRMKINLGNGAAIHPSDTSFSRADIDLK"
     gene            462..914
                     /gene="rpiB"
                     /locus_tag="PK754_14570"
     CDS             462..914
                     /gene="rpiB"
                     /locus_tag="PK754_14570"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNB10773.1"
                     /translation="MKIAIGCDHGGFPLKATVIETVQKAGHETMDLGTFSSDPADYPD
                     YSEKVAQAVQTGLADRGIVICGSGVGATVAANKFKGIRAGLCHDTFSARQGVEDDDMN
                     VLTLGARIIGPLLAIEVIQAFLKARFSNAERHLRRLNKVKGFEENFGK"
     gene            948..2045
                     /locus_tag="PK754_14575"
     CDS             948..2045
                     /locus_tag="PK754_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HNB10774.1"
                     /translation="MEKPYPPVYYADYLELNQLLGAQHPKSDAYGKPAHDEMLFIIIH
                     QAYELWFKQILHEVDSVARLFAEDHIDEKNIGIAVARLQRVTEIQKILVDQLRILETM
                     TPLDFLEFRDFLVPASGFQSVQFRLIENKLGLKHSQRLNYGQHPYHDRLSKEHQSLVQ
                     TAEQSASLLELVERWLERTPFLELGDFNFWSAYRGAVTRMLDEDHRTIEHNVSLSPSK
                     KEEQLKQLDLTRINFDALFDENKHNQLMKEGVRTISYRATQAALLICLYRDQPILHQP
                     FRLITLLVDIDEMFTTWRYRHALMVHRMIGTKIGTGGSSGHQYLKGTSDQHKIFSDFF
                     NLSTFLIPRSSLPKLPGDVERNLGFVYSQKQ"
     gene            2070..>3194
                     /locus_tag="PK754_14580"
     CDS             2070..>3194
                     /locus_tag="PK754_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNB10775.1"
                     /translation="MSAIVDMIRKAGVVGAGGAGFPAHIKAGSTVDTVIANGAECEPL
                     IHKDYELMLHYPAEVVRGVELVMQSTGAKEGILGVKEKNTKAIEALQKHLKPGIRLHL
                     LGDFYPSGDEYILVYESTKRLIPPQGIPLHVGCVVNNVETFYNIARAEKGEAVTEKFI
                     SVSGAVKKPFTGIVPVGATFADVIAMAGGITEEEYGVFVSGIMMGKLTFDLDQPITKT
                     CAGLIVLPKEHKLVTRKSLPEESMHRIGKSACDQCSYCTELCPRYILGYDIQPHKVMR
                     SLGFTKTGTDYWNQFASLCCACGLCTLYACPESLFPKEACDQSKRVMKEQNIKWSGPV
                     EIQPHPMYEGRRTPLKMLVKRLGVTDYDIHAPYSDRQATPF"
BASE COUNT          868 a          780 c          751 g          795 t
ORIGIN      
        1 ggggtgttga caaaacctca cttcagggtt attcttatca gggaatgcga tgggaagatc
       61 cggcggattc tctgcgtatc ctgagcggta caaataccgg cgatatccga aagtggaacc
      121 gtgaagagtt ttttttatta cgccaccatg tgaagttttt ggcaacggat aaggcagggc
      181 cgcagattta taatttgcaa tccgacccgt atgaagaaca caacttgtgg gacgatttat
      241 cttttagaga agcgcatcgg ccatggatac atcaatacat atccgaccat gcggtgctcg
      301 cagaattggc caaacgaatg aaaattaatt tgggaaacgg agccgccata cacccatcgg
      361 atacttcgtt ttcgcgtgcg gatattgatt tgaaataata cgaaaagttt atatatatag
      421 ccgattcatt caacaatgtc ccatttgctg aagaggtaat catgaaaata gctattggtt
      481 gtgatcacgg cgggtttccc ctcaaagcga cggtgatcga aactgttcag aaggccggtc
      541 acgaaaccat ggatctggga acattttcat cggatcctgc ggattatccc gattattcgg
      601 aaaaagtagc ccaggcggta caaacgggtc tcgccgatcg cggtatcgtc atctgcggca
      661 gcggtgtcgg cgctacggtg gcggccaata aattcaaagg cattcgcgcc ggattatgtc
      721 atgatacatt ttcggcccgg caaggcgtag aggatgacga catgaatgtg ctcaccctcg
      781 gtgcgcgtat tatcggtccg cttttggcca tcgaagtaat ccaagcattt ctcaaagcgc
      841 gcttttccaa cgccgaacgt cacctccggc ggttgaataa agtcaaaggt tttgaagaaa
      901 atttcggcaa ataaaaattc ttatttaatt ttttaactac tggtcctatg gaaaaacctt
      961 atccgccggt ttattacgcc gactatttgg aactcaatca actcctcggc gcacaacatc
     1021 ctaaaagcga cgcatatggc aaaccggcac acgatgagat gctgtttatc atcattcatc
     1081 aggcgtacga actttggttc aaacaaattt tgcacgaagt agattccgtt gcgcgtttgt
     1141 ttgcagaaga tcatattgat gaaaaaaaca tcggcatcgc cgtcgccaga ttgcagcgcg
     1201 tcacggaaat ccagaaaatc ctggtagatc aactgcggat tttggaaacg atgacaccgt
     1261 tggatttttt ggaatttcgt gattttttag tcccggcttc gggttttcaa agcgtccagt
     1321 ttcgcctgat cgaaaacaaa ctcggcctca aacattccca gcggctcaat tacggtcaac
     1381 atccatacca cgatcggctc tccaaagaac atcaatcttt agtgcagact gctgaacaat
     1441 cggcttcgct gctcgaattg gtcgagcgct ggttggaacg cacaccgttt ttggaattgg
     1501 gtgattttaa tttttggagc gcataccggg gtgccgtaac gcgcatgctg gatgaagatc
     1561 accgcaccat cgaacataac gtaagtcttt cgccatccaa aaaagaagaa caattaaaac
     1621 aacttgatct cacgcggatc aattttgatg cactcttcga tgaaaacaaa cataaccaac
     1681 tgatgaagga aggcgtacgc acgatttcgt accgcgccac gcaggcagcg ctactgattt
     1741 gtttataccg cgatcaaccg atattgcatc agccgtttcg cctcattact ttacttgtgg
     1801 atattgatga aatgtttaca acgtggcgtt atcgccatgc actgatggta caccgcatga
     1861 tcggcaccaa aatcggcacg ggcggttcgt ccgggcatca gtacctgaaa ggcacttcgg
     1921 atcaacataa aatcttttcc gattttttca atctttcgac atttttaatt ccgcgttcct
     1981 cattacccaa gctgcccggc gacgtcgagc gcaatcttgg atttgtctac agtcaaaaac
     2041 aataaggatc gttaaccgat aaaacaccta tgagcgctat tgttgatatg atacgcaaag
     2101 ccggtgtggt cggcgccggc ggagccggtt ttccggcgca catcaaagcc ggatccaccg
     2161 tggataccgt aatcgccaac ggcgccgaat gcgagccgct gattcacaaa gattacgaat
     2221 tgatgttgca ttatccggcg gaagtcgtgc gtggcgtcga actcgtcatg caatccacgg
     2281 gcgccaaaga aggcatcctc ggcgtcaaag aaaaaaacac caaagccatc gaagcactac
     2341 agaaacatct caaacccggc atacggcttc atctgctggg tgatttttat ccttcgggcg
     2401 acgaatatat cctcgtttat gaaagcacca aacgcctgat acctccgcaa ggcataccgc
     2461 ttcatgtcgg ctgcgtggtc aacaatgtcg aaacgtttta caacatcgcg cgggccgaaa
     2521 aaggcgaagc cgtgacggaa aaattcatct ccgtttccgg cgcggtaaaa aaaccgttca
     2581 ccgggatcgt tcccgtcggt gcgacatttg ccgatgtgat cgccatggcc ggcggcatta
     2641 cagaagaaga atacggtgtt tttgtctccg ggatcatgat gggcaaactg acctttgatc
     2701 tggatcaacc catcaccaaa acctgcgccg gattgatcgt acttcccaaa gagcataaac
     2761 tcgtgacgcg aaaatctcta ccggaagaat ccatgcatcg tatcggcaaa tcggcttgcg
     2821 atcaatgcag ctattgtact gaattgtgtc cccgttatat tttgggttat gatattcagc
     2881 cgcacaaagt gatgcgcagc ctcggattta ccaaaaccgg tacggattat tggaatcaat
     2941 ttgcctcttt gtgttgtgcc tgcggcttat gtacactgta tgcatgtccg gaaagtctct
     3001 tccccaaaga agcctgcgat caaagcaaac gtgtgatgaa agaacaaaat atcaagtggt
     3061 caggcccggt ggaaatacaa ccgcatccga tgtacgaagg gcgccgcacc ccgctcaaaa
     3121 tgctggtgaa acgtcttggc gtcacggatt atgacataca tgcgccttat tccgatcgtc
     3181 aagcaacccc attt
//
LOCUS       DAOQNQ010000345         3193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_15306_length_3193_cov_5.632887, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000345 DAOQNQ010000000
VERSION     DAOQNQ010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15306_length_3193_cov_5.632887"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PK754_14585"
     CDS             <1..183
                     /locus_tag="PK754_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10776.1"
                     /translation="DVAAVTVDQKNFWGVKVIYFVTATVPNKYVDINGEQKEVAGNLN
                     FDYLGLPLWISRTSKS"
     gene            352..1080
                     /locus_tag="PK754_14590"
     CDS             352..1080
                     /locus_tag="PK754_14590"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNB10777.1"
                     /translation="MRFCFLMLCTLSFTGCDKEPVTEDVQITRGRELFHSLQFSSDGK
                     TSCATCHPEGHTDNRNWHFAAIHDTTAGIPDSFRTITLWGIAQTGSPYLWKNSFRSLE
                     SVTRLYTDTIMGGTASEDEVAALVAYQRSLAFPKNPNLADNGQLTEAQARGKSIYEDP
                     KRGNCAPCHPAPTYGSPIPKNIYTGGTFRTVGLRGLYSIAPYFHNGSAKTLHEVIEHY
                     AGNGLIAPLTAQERDDLVEYLKTL"
     gene            1102..1908
                     /locus_tag="PK754_14595"
     CDS             1102..1908
                     /locus_tag="PK754_14595"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB10778.1"
                     /translation="MKHDKMRFVLILSLIFSVMQSCEKEKINEDAQIAYGRKLFNDTK
                     FSGSGQLSCGTCHPQGDMDNEQWHLDLIHDSLGGVAVSYSTPTLFGVAETAPYLWKGQ
                     GGSDLDTLTKTVITGIMGGTATDEEIKAIGAYMRSLDVPANPFRNDDGSLTTEQARGK
                     IVFENQGKCNICHSGETLSGKFSIQIKAARPAFDVPSLRWVFATAPYWHDGSAGSLRA
                     VINHYADTITTAQMTTWKWNGAGQVTPKPIFDIELTEQEKNDLIEYLKTL"
     gene            1933..3141
                     /locus_tag="PK754_14600"
     CDS             1933..3141
                     /locus_tag="PK754_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10779.1"
                     /translation="MSKFCWIVIAVCLLAAPIHAQEETVAPNTESGTEGTSVGFGGFV
                     DVAHAVGRKAGNANTLGDNNFNNMRTLLMFNAQQGSKIRADVEVLFDDRSKDRIRLQG
                     AFVTIFDVPNEAVNFMIGKIPNPFGNFAKREFSDVNPLIGQPLMRQYRTALDWNNLWN
                     NAEQIARKSERKKYDGNIPISRFEAATPTVYDARWDFGVQMFGTIGWFEYQAAVTEGS
                     ISNPEADLNKGKQFVGRIGFNPVPGIRIGFSGAYNPYLSKADDQRLLEEGKGLGEYHQ
                     TAYGADWELSYRYFMMSGEFVRSTWDATVIEGELSNWSIYSDIKYKLHPRWYIAGRYD
                     IMRFSEIRESKLSSKKTPWDYNVQRYEAGIGFRVTTDVTLKLVEQWTRFDQSEIPMSR
                     LTAVQLSVPF"
BASE COUNT          893 a          633 c          794 g          873 t
ORIGIN      
        1 gatgtagccg ctgttactgt ggatcaaaag aatttttggg gtgtcaaagt tatttatttt
       61 gtaacggcga cggttcccaa taaatacgta gatatcaacg gtgaacaaaa ggaagtggcg
      121 ggaaatctca attttgatta tttaggattg ccactttgga tttcacgaac aagcaaatct
      181 taataagata aaaatataag attacaaaaa aacctccgat aaggaggttt ttttgtttta
      241 ccggtatagt ataaaaaacg gcgcttgtgt acgcggggaa tttagcgtat gtttgtgcgt
      301 tgtaatcgtc tcaatactcc ataaaaatat atctatatca ggcgatacgt tatgcgattt
      361 tgttttttaa tgttatgcac attgagtttc accggttgtg acaaagaacc tgttaccgaa
      421 gatgtacaga taacacgcgg gcgtgaactg tttcacagcc ttcaattttc gtcggacggt
      481 aaaacgtcct gcgcgacatg tcaccccgaa gggcacacgg ataaccgtaa ctggcatttt
      541 gcagcgatcc acgacacgac ggccggtatt cccgattcat ttcgcaccat tactttatgg
      601 ggcatagcac aaaccggatc gccgtatctt tggaaaaact cctttcgttc gttggaatcc
      661 gttacgcgtt tatatacgga tactattatg ggtgggacgg cctcggagga tgaagtggcc
      721 gctttagtgg cgtatcaaag atcgctggca tttccaaaaa atccgaactt ggcggacaac
      781 ggtcaactga cagaggcgca agcacgcgga aagagcattt acgaagaccc caaacgcggc
      841 aactgtgcgc catgccatcc tgctccgacc tacggaagtc ctattccgaa aaatatttat
      901 accggcggca catttcgtac tgtcggatta cgtggtcttt attcgatagc accttatttt
      961 cataatggtt cggccaaaac actgcacgag gtgatagaac attatgccgg caatgggctt
     1021 attgcaccgt tgaccgcgca ggaacgtgac gatctcgtag aatatcttaa aactttataa
     1081 tatctaacga tcagtacagc tatgaaacat gacaaaatga gatttgtact tattctgagt
     1141 ttgatttttt cggttatgca atcttgtgag aaagaaaaaa tcaatgaaga cgcacaaatc
     1201 gcttatggaa gaaaactttt taacgatact aagttttccg gcagcggaca gttaagctgc
     1261 ggtacttgtc accctcaagg tgatatggat aacgagcagt ggcatttgga cctgatccat
     1321 gattcacttg gcggtgtggc ggtatcctat tccactccga ccttatttgg cgtagcggag
     1381 acggcgccct atctatggaa aggtcaaggt ggcagcgatc tggatacgtt gaccaaaacg
     1441 gttatcacgg gtattatggg cggtacggcc accgatgaag aaataaaagc catcggtgca
     1501 tatatgcgat ctttggatgt tccggccaat ccttttcgaa atgatgatgg ttctttaact
     1561 accgagcaag cgcgcggtaa aattgttttt gaaaatcagg gtaagtgtaa tatttgtcat
     1621 tccggagaga cgctctccgg taagttttcg attcaaatca aagcggcaag accggctttt
     1681 gatgtaccgt ctttgcgatg ggtttttgca acggcgccgt actggcatga cggatcggcc
     1741 gggtcattgc gcgcggtgat caatcattac gcagatacca tcactacggc gcagatgacg
     1801 acttggaagt ggaacggtgc cggacaagtg acgcccaaac ccatattcga tatcgaattg
     1861 acagagcagg aaaaaaacga tttgatcgaa tatctaaaaa cgttgtaatc aaattatttt
     1921 gaggatatgg ccatgagtaa attttgctgg attgtgattg cagtgtgttt attggcggca
     1981 ccgattcatg cacaggaaga aacggttgct ccgaatacgg aatccggaac ggaaggaaca
     2041 tccgtaggtt ttggcggttt tgtggatgtc gcgcatgccg tcgggcgtaa agcggggaat
     2101 gccaatacgc ttggggataa taattttaac aatatgcgca cattgttgat gtttaatgca
     2161 caacagggtt cgaaaatacg cgccgatgta gaagttcttt ttgatgatcg ttctaaggat
     2221 cgaattcgct tgcaaggtgc atttgtgaca atttttgatg tacccaatga agccgtcaat
     2281 tttatgatcg gaaaaattcc gaatccgttt ggtaactttg ccaagcgtga attttccgat
     2341 gtcaatcctt tgatcggtca acccctgatg cggcaatacc gcacggcatt ggattggaat
     2401 aatctctgga acaatgcgga acagattgcc cgaaagagtg aacgaaaaaa atatgatggc
     2461 aatataccga tttcgcgatt tgaagcggca acgccgacgg tatacgatgc gcgatgggat
     2521 tttggtgtac aaatgttcgg tacgatcggt tggtttgaat accaggcggc tgtcaccgaa
     2581 ggttccatat ccaatccgga ggcggatctg aataaaggca aacaatttgt aggtcgtatc
     2641 ggttttaacc cggttcccgg tattcgtatc ggtttttccg gagcatacaa tccgtatttg
     2701 tcgaaagccg atgaccagcg attattggaa gaaggtaaag gactaggcga gtaccatcag
     2761 acggcgtacg gtgcggattg ggaactgagc tatcgctatt ttatgatgag cggtgaattt
     2821 gtacgcagca cttgggatgc gacggtgatc gaaggcgaac tgtctaactg gagcatttat
     2881 tctgatatca agtacaaact gcatccaaga tggtatatcg caggacggta cgatattatg
     2941 cgtttttccg aaatccgtga atctaaatta agttctaaaa aaacgccgtg ggattataat
     3001 gttcaacgtt atgaagcggg tatcggattt cgtgtgacaa ccgatgtgac attgaagctt
     3061 gtcgaacagt ggacgcggtt tgatcaatcg gaaattccga tgagccgttt gacggctgtt
     3121 caattatccg taccatttta aaagcattta cgaggattca tatgaaacta attttttcag
     3181 cgatagcggg cat
//
LOCUS       DAOQNQ010000346         3176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_15453_length_3176_cov_5.639218, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000346 DAOQNQ010000000
VERSION     DAOQNQ010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15453_length_3176_cov_5.639218"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PK754_14605"
     CDS             complement(<1..628)
                     /locus_tag="PK754_14605"
                     /inference="COORDINATES: protein motif:HMM:NF025114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNB10780.1"
                     /translation="MDTFKFDVVIIGAGPTGLSCAVECARAGLSHVVLEKGCIVNSIY
                     HFPSNMIFFTTSDLLEIGGVPFTTPNPKPTREEGLQYYKRVVQYYSLNVRTGEKVISI
                     QNLSNEFLVDTESNDQQKRYTARHIVIATGYYDQPNKLFVPGEESSKVSHYYTDPHPF
                     YGKNVLIVGGKNSACIAALELYRYGAKVTVIHRGEEIKKSVKYWILPDF"
     gene            complement(628..2496)
                     /gene="dnaG"
                     /locus_tag="PK754_14610"
     CDS             complement(628..2496)
                     /gene="dnaG"
                     /locus_tag="PK754_14610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNB10781.1"
                     /translation="MAKIPQHTIDEVKNAWDVVELVSGYVGLKKKGHNFWGLCPFHNE
                     KTPSFSVNSDKQIFKCFGCGAGGDVISFIMQYEKTSYPEAIRFLAEKKNIPIPQDHSP
                     EEINQRSEIEELYFINKLTAAFFAEQLTLPQGDEARQYLRKRGFDDAVIAAYGIGYAP
                     DRWDGLFEYAKKESVDPRLLERAGLIIPKTGGYYDRFRHRLMFPISNVSGRVIAFGGR
                     QMRDEPNSPKYLNSPESAIYHKSKVLYGLAQAKDGIRQMDEVIIVEGYADCISLFQFG
                     IHNVAASSGTAFTAEQAQLIRRYTENVCLVYDGDQAGIRAAERGGAIMLEAGLNVKIV
                     VLPGEHDPDSYVREAGESAMQQLVQKGQDYINFRVVEWLKSDKLRTANDKANAVSEMA
                     QIIARMDDSLKRNAFIEEVARKLDTALPLVAKAVQKNRSAVARQKDKEPIVISHAPAS
                     SLTTASSSVPEKVLRAERGILRELLLNPSQCEYVFGYVEPRDFKNPAIQTLIGKIHSL
                     YMEETPYDYNVVVQDCEEKIKTAAERLIFGAGPRQAIVEDKYELSECIREMQLYDLQL
                     EEQTVREAIRVRELEKEDPAELMASLRHIADRIKAVRHSKLISLYETGEHTDEVPF"
     gene            complement(2498..>3176)
                     /locus_tag="PK754_14615"
     CDS             complement(2498..>3176)
                     /locus_tag="PK754_14615"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB10782.1"
                     /translation="SYFLGRMARLRGDYTKAITYFNQSVTIADQCVLFKMVDGWVAGS
                     ALYYLAETYELSGDRTKAEIWYEKARDDARSGKGIVQGAKNRLKYPIGEFEMEFIKIR
                     QKALIQPEPSQLITLNTMLASASNIPEMAKYVSQIHYYIGRTQFALENYPEAERHFTA
                     SLDTQTDDGEEKWRTAFNRYYRGAARVKLGKKSEAKSDLKLLTDDASYVDGNRIRFRA
                     QQLLKGL"
BASE COUNT          815 a          809 c          685 g          867 t
ORIGIN      
        1 taaaatccgg tagtatccag tatttcacgc tcttttttat ttcttcgccc cgatgaatga
       61 cggtgacttt cgctccgtag cgatataatt ctaatgcagc gatacaagcc gaatttttac
      121 cgccgacaat caacacgttt tttccataaa agggatgcgg atcggtataa taatgcgata
      181 ccttcgacga ttcttcgccc ggtacaaata atttattggg ttgatcataa taacccgtcg
      241 caatcacgat atgccgcgca gtataacgct tctgttgatc attcgattcg gtgtccacca
      301 aaaattcgtt tgatagattt tgaatcgaaa tcactttttc gccggtacga acattcaaag
      361 aataatattg aacgacacgt ttataatatt gaagtccttc ttcccgtgtc ggtttaggat
      421 tcggtgtagt aaaaggtaca ccgccgattt ccaaaagatc actggtcgta aaaaaaatca
      481 tgttggaggg aaagtgatag atcgaattga cgatacaacc tttttcgagc accacatgcg
      541 acaaaccggc acgcgcacat tcgacggcgc aggatagccc ggtcggcccg gcaccgatga
      601 tcaccacatc aaacttaaac gtatccatca aaacggcacc tcatcggtat gctcgccggt
      661 ttcatagagc gaaatgagtt tactatgacg aacggccttg atacgatccg cgatatgtcg
      721 aagcgaagcc atgagttcgg cagggtcttc tttttccaat tcacgtacac gaatcgcttc
      781 acgtaccgtc tgttcctcta attgtaaatc gtacaactgc atttcgcgaa tgcattcgga
      841 aagttcgtat ttgtcctcca cgatagcctg gcgcggaccg gctccgaaaa tcagcctttc
      901 ggccgccgtt ttgatttttt cctcacaatc ctgtacgacc acattgtagt cgtaaggcgt
      961 ttcttccata taaagcgagt ggatttttcc gatcaacgtt tgtatggccg gatttttgaa
     1021 atcgcgcggt tcgacataac cgaaaacgta ttcgcattgc gaagggttaa gcaacagttc
     1081 acgcaggata ccgcgctccg cacgcaaaac tttttcggga acagatgacg acgccgtagt
     1141 caatgaactc gccggagcat gcgaaatgac gatcggttct ttatcttttt ggcgtgccac
     1201 ggcgctgcga tttttttgta ccgcctttgc aaccaacggt aacgcggtat ccaatttgcg
     1261 cgccacttct tcgatgaagg cattacgctt gagactgtcg tccatccgcg caatgatctg
     1321 cgccatctcc gaaaccgcat tggccttatc gttagccgta cgaagtttat ccgatttcaa
     1381 ccattcgact acacgaaaat tgatataatc ttgccccttt tgcaccaact gttgcatcgc
     1441 gctttcaccg gcttcacgca cataggaatc cggatcatgt tctccgggca gaacgacgat
     1501 tttgacattg aggccggctt ccagcatgat agcgccaccg cgttcggccg cacgaatgcc
     1561 tgcctgatcg ccgtcgtaca ccagacatac attttcggta tatcgccgaa tgagctgcgc
     1621 ttgctctgcc gtaaatgccg taccgctcga tgccgcaaca ttatgaatac caaattgaaa
     1681 caaacttata caatcggcat aaccttcgac gatgatcact tcgtccatct gacgaatgcc
     1741 gtccttggct tgcgctaagc catacagcac tttgctttta tgataaatcg cactttccgg
     1801 cgaattaaga tatttcggtg aattcggttc atcccgcatt tggcggcctc caaatgcgat
     1861 cacacgcccg gaaacattgc tgatgggaaa catgaggcga tggcgaaagc gatcataata
     1921 accgcccgtc ttaggaatga tcaacccggc gcgctcaagt aagcgcggat ctaccgattc
     1981 ttttttggca tactcgaaca atccgtccca acgatccggt gcatatccga taccgtacgc
     2041 ggcgatcacg gcatcatcaa aaccgcgttt ccgcaaatac tgacgcgctt catcgccttg
     2101 aggtagagtc aattgctcgg caaaaaaagc ggcggtcagt ttgtttataa aatataattc
     2161 ttcgatttcc gaacgttgat tgatttcctc cggagaatgg tcttgcggaa tcgggatatt
     2221 ttttttctcg gcgaggaatc gtatggcttc cggataactc gttttttcgt attgcatgat
     2281 aaacgaaatc acatcaccac cggctccgca accgaaacat ttgaaaattt gtttatcgga
     2341 attaacggaa aatgaaggcg ttttttcgtt gtgaaaagga cacaaccccc aaaaattgtg
     2401 acctttcttt ttgagcccga cataacccga tacgagttcg accacgtccc atgcattttt
     2461 cacttcatct atcgtgtgtt gaggaatctt cgccatgcta caatcctttc aacaactgct
     2521 gagcacgaaa acgaatacga tttccgtcaa catacgaagc gtcatccgtc agaagcttca
     2581 aatcggattt ggcttcactt tttttaccaa gcttgacccg tgccgcgccg cgatagtaac
     2641 ggttaaatgc cgtgcgccat ttttcttcac cgtcatccgt ttgtgtatcg agcgaagcgg
     2701 taaagtgacg ctccgcttcc ggataatttt ccagtgcaaa ttgtgtacgc ccgatataat
     2761 aatgaatctg cgaaacatac tttgccattt ccggtatatt ggaagcggaa gccagcattg
     2821 tattgagcgt gatcagttgc gaaggttccg gctggatgag cgctttctgt cggattttga
     2881 taaactccat ttcaaattcg ccgatcggat atttcaggcg atttttagcg ccttgtacga
     2941 tacctttgcc gctgcgcgca tcatcccgcg ctttttcgta ccaaatctca gcctttgtac
     3001 gatctccgga taattcgtag gtctcagcca aataatacaa cgcagaaccg gcaacccagc
     3061 catctaccat tttaaagaga acgcattgat cggcgatcgt tacggattga ttaaaatagg
     3121 taatcgcttt agtgtagtcg cctcgcaagc gtgccatccg gcctaaaaaa tacgag
//
LOCUS       DAOQNQ010000347         3149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_15706_length_3149_cov_5.977699, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000347 DAOQNQ010000000
VERSION     DAOQNQ010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15706_length_3149_cov_5.977699"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PK754_14620"
     CDS             complement(<1..513)
                     /locus_tag="PK754_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004778917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNB10783.1"
                     /translation="MSFRIETDSMGEVQVPSDRYYGAQTARSLSNFKIGGDRFPREMI
                     RALGILKKSAAIANQELGTLTKDKAELIIKACEEVIAGKLDDHFPLVVWQTGSGTQTN
                     MNANEVISNRAIEISGGTMGSKKPIHPNDDVNKAQSSNDTFPTAMHIAAAERIVHHLI
                     PKVKLLRTTLQ"
     gene            complement(532..1074)
                     /locus_tag="PK754_14625"
     CDS             complement(532..1074)
                     /locus_tag="PK754_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10784.1"
                     /translation="MKSFIVLLTVIGVIVSSCGPQKATRSDMSKLDEARANAEKAKEN
                     LANTQNASQMAADELAKKKDENEKSKDELRKVRANLKKMGQWADYMPFNKENNTLLLP
                     GGVTRTIRKEITDTEALTQLNAMLDAQATKQDKDHHALTTCIGGFETCLNGKIVVLYF
                     TKDDAGACKRTWYETENNCD"
     gene            complement(1114..1491)
                     /locus_tag="PK754_14630"
     CDS             complement(1114..1491)
                     /locus_tag="PK754_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10785.1"
                     /translation="MNQIINGTGASLHGIEQACITGNGSLSEACRDGAIQVYVAFRNK
                     INMSCSLVDVHSLDGLHDSVAVKIQSSRISPAEIYDRLASGQPSIMGYISQNSFHLDF
                     RYIRKEEVADLLRRLKIVLEIES"
     gene            complement(1513..>3149)
                     /locus_tag="PK754_14635"
     CDS             complement(1513..>3149)
                     /locus_tag="PK754_14635"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HNB10786.1"
                     /translation="NAKPQVNLIERWVTAYLDKKLPSIEIRHASWIHLEERTVWKKRK
                     PVSEWGESLRLDEMHISLKESLFRDAEFDLTGTVYRGKQKNGVTISGTMDHQDHALYV
                     KSFFDSDFRIPFLENWTDAVIALKGVEFKIHSIEGYGNQNTLKAKLDIQNLDIVSESL
                     SDEKLKDINVGFDLHITADPDSIIIHPETRVYLNTIHAFFSGVAKPFRKFPVWDVTMR
                     MPPTTMDDFLSSIPKPIIRRLDGMRVKGQFDYQARFRLDLAQIDSIRFDPALHISSDF
                     KVETYGDSIHVYALRDTFLYHFKTEMEKDSMVWVGPLNAYYMPYDSIPPLVMQAVIAC
                     EDNSFFKNDGFNVLQIERSMRDNIREKKFARGASTISMQLVKNIFLSREKTISRKFQE
                     MMITWLMNHDLALDSLKKNKEKHKKRLMEIYLNIVEWGPDVHGIAHAAEFYFKKKPKE
                     LTVPEAVFLATILPNPKKYERYFESGKSKKKHQDFMNLIVRMLEDKKVIDSVAMQAAI
                     PVTFTVRGSAQRWIKDYALHDSTDSDMEFSLPTIRE"
BASE COUNT          802 a          717 c          646 g          984 t
ORIGIN      
        1 ttggagtgtc gtgcgaagca attttacttt gggaatcaag tgatgcacga tgcgttctgc
       61 ggcggcgata tgcatggctg taggaaatgt atcattggac gactgggctt tatttacatc
      121 atcattagga tgaatgggtt ttttactgcc catcgtgccg ccggaaattt caatagcgcg
      181 attggaaatc acctcattgg cattcatatt ggtttgtgtg ccgctccccg tctgccatac
      241 gaccaaagga aaatgatcat ccaattttcc ggcaatcact tcttcacagg ctttgatgat
      301 caattcggct ttatctttgg tcaacgtccc tagttcctga ttggctatcg cggcggattt
      361 tttcaaaata ccgagtgcac gtatcatttc acgcggaaag cgatcgccgc ctattttaaa
      421 attggaaagt gaacgcgccg tttgagcgcc ataataacga tccgacggga cttgtacttc
      481 acccatgctg tcggtttcaa ttcgaaaact catgtcgtat ttctcctgaa tttaatcgca
      541 gttattttcg gtttcatacc acgtgcgttt gcacgcaccg gcatcatctt tggtaaaata
      601 taaaacaaca attttcccgt taaggcatgt ctcaaatccg ccaatgcatg tagttaacgc
      661 atgatgatct ttatcttgtt tggtcgcttg cgcatcaagc atagcattga gttgggtcaa
      721 ggcttccgta tccgttattt ctttacgaat agtgcgtgta actccgccgg gcagtaaaag
      781 agtattattt tctttattga acggcatata atcggcccat tgacccatct ttttcagatt
      841 agcacgcact ttgcgcagtt catctttaga tttttcattt tcgtctttct tcttggccag
      901 ttcatcggcg gccatctggg aagcattttg cgtattagct aaattttctt ttgctttttc
      961 cgcattagca cgcgcttcat ccagtttgct catatctgat cgagttgctt tttgggggcc
     1021 acatgaggat acgatcacac cgataaccgt caataatact atgaatgatt tcatgtttat
     1081 ttctccgtaa aataatgtat aaacatactg gttctatgac tctatttcta atactatttt
     1141 taatctacga agcaaatccg ccacctcttc tttgcgaata taacggaaat caagatgaaa
     1201 tgaattttgt gaaatatacc ccataatgga cggttggcct gaagcgaggc gatcatagat
     1261 ttccgcaggt gatattcgcg aagattgtat tttgacggca acactgtcat gaagcccgtc
     1321 taaagaatga acatccacaa gcgaacaaga catgttaatt ttattacgaa aagctacgta
     1381 cacctgaatc gctccatcac ggcaggcttc tgataaagac ccattgccgg tgatacacgc
     1441 ttgttcgata ccgtgtagtg atgcccccgt tccattgatt atttgattca ttcgcatggt
     1501 ccgataaaat ttttattctc tgatcgttgg caatgaaaat tccatatcgc tgtccgtcga
     1561 atcatgcagg gcatagtctt taatccatcg ttgtgccgaa ccgcgcaccg taaatgtaac
     1621 cggaatggcc gcctgcatag ccactgaatc tattactttt ttatcttcca gcatacgcac
     1681 gatcaggttc ataaaatcct ggtgcttctt tttggatttg ccggattcaa aatagcgttc
     1741 atactttttg ggattgggca atatcgtagc caaaaacacc gcttcgggta ccgtcaattc
     1801 cttcggtttt ttcttaaaat aaaattccgc cgcgtgcgcg ataccgtgca catcgggccc
     1861 ccactctacg atattgaggt atatctccat caatcgtttt ttatgttttt ctttgttttt
     1921 tttcagcgaa tccagcgcca ggtcgtgatt catcagccag gtgatcatca tttcttggaa
     1981 tttgcggcta atcgtttttt cacggctcag aaaaatattc ttaactaatt gcatcgaaat
     2041 cgtactggcg ccgcgtgcga attttttttc acgtatatta tcccgcatag aacgttcgat
     2101 ctgaagcaca ttgaatccgt catttttgaa aaaggaattg tcttcacacg caatgacggc
     2161 ctgcatgacc agcggcggta tactgtcgta aggcatgtaa tacgcattta gcggaccgac
     2221 ccataccatg ctgtcctttt ccatctcggt tttaaaatgg tacaaaaacg tatcgcgcaa
     2281 tgcgtaaaca tgaatggaat cgccgtatgt ttcaacctta aaatcactgc tgatatgcaa
     2341 tgcgggatca aaccggatgc tgtcaatctg cgccaaatca aggcggaacc gtgcttggta
     2401 gtcaaattga ccttttacac gcattccgtc taagcgacgt attatcggct taggtatcga
     2461 acttaaaaaa tcatccattg ttgtgggcgg cattctcatc gtgacgtccc acacgggaaa
     2521 ttttctaaat ggtttggcta caccggagaa aaaggcatga atcgtattga ggtaaacccg
     2581 tgtttcgggg tgaataatga tactgtccgg gtccgctgta atgtgaagat caaaaccgac
     2641 gttaatgtct tttagttttt catctgaaag cgattccgat acaatatcga ggttttgtat
     2701 atctaatttg gctttgagcg tattctgatt accatatcct tcgatgctgt gtattttaaa
     2761 ttcaacgccc ttgagcgcaa tcacggcatc ggtccaattt tccaaaaaag gaattcggaa
     2821 atcggaatca aaaaaacttt tgacgtatag cgcatgatct tggtgatcca tggtaccgga
     2881 tatcgtaacg ccattttttt gttttccgcg atataccgta ccggtcaaat caaattcagc
     2941 atcccgaaac agactttctt taagactgat gtgcatctcg tccagccgca ggctttcacc
     3001 ccattcggat accggtttac gtttcttcca caccgttcgt tcctcgagat gaatccaaga
     3061 cgcgtgacgg atttctatag acggtaattt cttgtccaaa tacgccgtca cccagcgttc
     3121 gatcagattc acttgcggct tcgcattgg
//
LOCUS       DAOQNQ010000348         3134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_15877_length_3134_cov_5.887301, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000348 DAOQNQ010000000
VERSION     DAOQNQ010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15877_length_3134_cov_5.887301"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PK754_14640"
     CDS             <1..558
                     /locus_tag="PK754_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNB10787.1"
                     /translation="LKLGLLHGKMSSAEKQAVMADFKKKATDILVSTTVIEVGVNVPN
                     ATIMVIEHAERFGLTQMHQLRGRVGRGADQSYCILMVGKHHLMEETEQRLRIMEETTD
                     GFKIAEEDLRLRGPGEFFGTKQSGMPELRLANIVEDRELMALAKEAAFTIVNLDPQLR
                     SATHKTVRKKFLEAYRDRMDLSQIG"
     gene            complement(555..>3134)
                     /gene="mutS"
                     /locus_tag="PK754_14645"
     CDS             complement(555..>3134)
                     /gene="mutS"
                     /locus_tag="PK754_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNB10788.1"
                     /translation="YRDTILLYRMGDFYETFDEDARTVHKVLGITLTKRSNGKASEVA
                     LAGFPYHALDSYLPKLIRAGFRVAVCEQMEDPKAAKGIVKRDVTEIVTPGTTLSEKIL
                     DQKTNNFLATVVTERRDDQTIFGLALADASTGEFFVGEFREKEFLDQLNLFIPAEMVV
                     PFSLYENMRMLLDRIKHRSVLTRQEDWLFSFQYAHDVLMAHFKTHSLKGFGVQDFTVG
                     LCAAGAGLHYLRETQRTDVSNVRHIGRLDDATYMTLDAVTLRNLEILQATADGYTHGG
                     LFSVLDHTETAMGGRMLKQWVVRPLKHTENIRKRLDAVEELTAEHGARDKVRQTLSRI
                     MDLERIIAKVCAGRVNPREIRALCESLKQIPELINNLSNFKSDAIRGAVQAIHILDQL
                     TDHIDNALVDEPSVQIKDGQVIREGYNEELDVLRRMAFDGKSYIAGIQQRERERTGIA
                     SLKVQFNNVFGYYIEITHTHKDKVPPDYIRKQTMTNAERFITPELKEYEEKILTAEDK
                     IIRIETELFDAIRKTVAQTAAEIQINAQRIAELDCYASLAEAAVQNKYVKPVVDDSAA
                     LIIKAGRHPVVERILPPDAPFVPNDLQMNEEEQIHLITGPNMAGKSCYLRQVGLIVLL
                     AQVGSFVPAQSAEIGITDKIFTRVGASDNLLGGESTFLVEMNETANILNNATSRSLIL
                     LDEIGRGTSTFDGLSIAWAITEYLHQHPAIRPKTLFATHYHELIELEELLPRVKNYNM
                     MVKKYDDKIVFVRKIVRGGSDHSYGIEVAKLAGLPASVITRAREVMGNLESHNISAHK
                     DKSELAGTSGDQSTQAQFTLFEDGTGLRLKTVLENLDVNSLTPLDALNHLNKLKAILT
                     EQS"
BASE COUNT          792 a          768 c          726 g          848 t
ORIGIN      
        1 ctgaaattgg gattgctgca cggcaaaatg agcagcgccg aaaagcaagc cgtcatggcc
       61 gacttcaaaa agaaagctac ggacatactc gtgagtacca cggtgatcga agtcggtgtc
      121 aacgttccca acgcgacgat catggtcatc gaacatgccg aacgttttgg acttacccaa
      181 atgcaccagt tgcgcggacg tgtaggtcgc ggcgccgatc aatcgtactg catactgatg
      241 gtcggcaaac atcacctgat ggaggaaacc gaacagcgac tgcgtatcat ggaagaaacg
      301 acagatggat ttaaaatcgc ggaagaagat ttgcgactgc gaggaccggg tgaatttttt
      361 ggaaccaaac aatccggcat gccggaactg cgtttagcca atattgtgga agatcgcgaa
      421 ttgatggcgc ttgccaaaga ggccgccttt acgatagtaa atcttgatcc gcaactgcgt
      481 agcgctaccc ataaaaccgt tcggaaaaaa tttttggagg cgtaccgtga tcggatggat
      541 ttgagtcaga tcggctaaga ttgttcagta agaatggctt tgagtttgtt cagatgattg
      601 agcgcgtcca ggggcgtgag actgttaaca tccaaatttt ctaatacggt ttttaaacgc
      661 aagcccgtgc catcttcaaa taacgtaaat tgagcctgcg tactctgatc gccggatgtg
      721 cccgcgagtt cggatttatc tttgtgcgcc gaaatgttgt gcgattccaa attacccatg
      781 acttctcgtg cacgggtaat caccgatgcc ggcaaccccg cgagtttggc tacctcaata
      841 ccatacgaat gatcacttcc gccgcggacg attttacgca cgaaaacaat tttatcgtcg
      901 tattttttta ccatcatatt gtaatttttt acgcgcggga gaagctcttc gagttctatc
      961 aattcatggt aatgggttgc aaacaaggtt tttggacgta tcgccgggtg ttgatgcaaa
     1021 tattcggtaa tggcccaagc aatggacaac ccgtcaaaag tgcttgtacc gcgacctatt
     1081 tcatctaaaa gaataagact gcgcgacgtg gcattattca ggatattcgc cgtttcattc
     1141 atttcgacaa ggaacgtact ttcaccgccg agcaaattat ccgaagcccc gacgcgcgta
     1201 aagattttat ccgtaatgcc gatctcagcc gactgtgccg gcacaaaact tccaacctgc
     1261 gccagtaata cgatcaatcc tacttgcctc agataacagc tttttcctgc catgttaggg
     1321 ccggttatca gatgtatttg ctcttcttca ttcatctgca gatcgttggg aacaaaaggc
     1381 gcatccgggg gaagaatgcg ttcgactaca ggatgacgcc ccgctttgat gataagtgct
     1441 gcgctatcgt caacgacagg cttgacatat ttattttgta ccgccgcttc ggccagtgaa
     1501 gcataacaat ccagctctgc aatccgttgt gcattgattt gaatttctgc tgccgtttgc
     1561 gccacagttt tgcgtattgc atcaaataat tccgtttcga tgcgaattat tttatcttcc
     1621 gcggtaagaa ttttttcttc gtattctttc agctccggcg tgataaatcg ttccgcattg
     1681 gtcatcgttt gcttacggat ataatccggc ggcactttgt ctttatgcgt gtgggtgatt
     1741 tctatgtaat aaccgaagac attattaaac tgtactttca aagatgcgat ccctgtacgt
     1801 tcgcgttcgc gttgctggat accggctata taacttttcc cgtcaaaagc catccggcgc
     1861 aacacgtcta attcttcatt gtacccttca cgaataacct gtccgtcttt gatttgtacc
     1921 gagggttcgt ccaccagtgc attatcaata tgatccgtca gttggtctaa aatatgtatc
     1981 gcctgcactg caccgcgtat cgcgtcggat ttaaaattgg ataggttatt tatcaattcg
     2041 ggaatctgct tgagcgattc gcacaacgca cgaatttcgc gcggattgac gcgcccggca
     2101 cacaccttgg cgatgattct ttcgaggtcc atgatgcgcg acaacgtttg tcgcactttg
     2161 tcccgtgcgc catgctcggc ggtcaattct tctaccgcat ccaatctttt ccggatattt
     2221 tcggtatgtt tcagaggacg cactacccat tgtttgagca tgcggccacc catggccgtt
     2281 tcggtatgat ccagtacgga aaatagccct ccgtgcgtgt aaccgtctgc ggtagcttgc
     2341 agtatttcca aattacgcag agtgaccgca tccaaggtca tgtaggttgc atcatccaat
     2401 cgaccaatgt gacgaacatt actgacatcg gtacgctgag tttcacgcag gtaatgcagt
     2461 ccggctccgg cggcacagag gccgaccgta aaatcctgca cgccgaaccc tttgagcgaa
     2521 tgcgtcttaa aatgcgccat caaaacgtcg tgcgcatatt gaaaagaaaa taaccaatct
     2581 tcctgacgcg tgagaacact acgatgtttg atgcggtcga gcaacatgcg catgttttca
     2641 tacagagaaa aagggacaac catttctgcc ggaataaaca aattaagctg atccagaaat
     2701 tccttttcac gaaattctcc gacaaaaaac tcccccgtcg aagcgtcagc taacgccaaa
     2761 ccgaatattg tttggtcgtc gcgacgttcg gtgacgaccg tcgcgagaaa attattggtc
     2821 ttttggtcta atattttttc ggaaagcgtt gtgcccggtg tgacgatttc cgtcacatca
     2881 cgtttgacta tgcctttggc cgctttcgga tcttccattt gttcgcacac ggctacccga
     2941 aatcctgcac gtattaattt aggcagataa ctatccagcg catgataagg aaaacctgcc
     3001 agcgctactt cggacgcttt gccgttggag cgtttggtca gagtgatacc gagaacttta
     3061 tgcactgtac gcgcatcttc gtcaaacgtt tcgtaaaagt ctcccatacg atacaaaaga
     3121 atcgtatcgc gata
//
LOCUS       DAOQNQ010000349         3130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_15903_length_3130_cov_5.496585, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000349 DAOQNQ010000000
VERSION     DAOQNQ010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15903_length_3130_cov_5.496585"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PK754_14650"
     CDS             complement(<1..464)
                     /locus_tag="PK754_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10789.1"
                     /translation="MLDIVLQRITSSESAQNLLRHIAEKKTIMVDGYLGSMDAVLGIL
                     AAQSAYVNDRLSVIVVPDSLTYGRVLDDLTTLQDYTTHKIPILQFPEDELLPYDDREA
                     NPAFVAARQESIEQLLNNEKAILVTTVRALSKRIPGPRSVASYKIELQSGAT"
     gene            complement(480..1937)
                     /gene="miaB"
                     /locus_tag="PK754_14655"
     CDS             complement(480..1937)
                     /gene="miaB"
                     /locus_tag="PK754_14655"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNB10790.1"
                     /translation="MSDTAFEEIHTPESIADVRSRLQGNRKRLYLETYGCQMNVYDSE
                     LVASIMRDMDYDLVDDYETADAIFMNTCAIRENAEQRVWGQLTRFKKLKEEKPHLIIG
                     VLGCMAKHLEEEIHAKRPYVNVVLGPDSYRKIPELIEKKQASQNLVQIRLPSQHGEDF
                     SLSEYRDFQLKPLQMDTRLSRTEVYDQVEPLRLGKITAWIAIMRGCDNFCTFCVVPYT
                     RGRERSRSVESIVEEVHKAVAQGYTEIGLLGQNVNSYRDPQQKDFAELMNRVSEVPGV
                     RRIRFTSPHPKDFPEHLLHIIAQRDNICKQLHIPVQSGSNRILELMNRTYTREEYLKL
                     IDTVRRIIPDVSISTDIITGFPSETERDHLDTLELFRTVQFDSAFMFKYSARIGTKAY
                     TMPDDVTEAEKSRRLQQIIEVQKENGLKSLHREIGKEREVLIEEVSRKSDNYYCARTD
                     KGQIIIIPKSDKTIGEYARIRIARADGHTLFGEYI"
     gene            complement(1942..2496)
                     /locus_tag="PK754_14660"
     CDS             complement(1942..2496)
                     /locus_tag="PK754_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10791.1"
                     /translation="MKKKKKTQPAVSKRVKSVIKKTDKTRSVALKTKKTKSEDEHKFN
                     LSPVDVERIAKSLGVEKQDFGDHYRMYLENQTEGRKLTLEIYPEIPIGRRKGTLITVY
                     TPNAHLQLHFCSGYVVSELLNEVTFVGVQNGKLSGLTIEKGAACSLYANVDTEMLSGD
                     LTTLGPEVMLSGIALSLTDSLINE"
     gene            complement(2493..>3130)
                     /locus_tag="PK754_14665"
     CDS             complement(2493..>3130)
                     /locus_tag="PK754_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10792.1"
                     /translation="ITRKKESAQDGAIEYYWDEYEPDYELTGNTTGRNALISFMNIFN
                     LAFSVLILKTYYRPLEDMPWMNESPWIGVFLGWVPFVFSLIFFIIPLFRSFAIIKQKR
                     NRRAMNVRKRIMRYLFSMPSASATVDEVVTHINGKSKGEEVLKKHDVQEALDSMLRDF
                     NGETTLNNNGDVRYTFPQIEQELKESELLRKNRSVDNDLGAVIVDTQNTQS"
BASE COUNT          841 a          730 c          637 g          922 t
ORIGIN      
        1 aacgtggcgc cgctctgaag ttctatcttg taagatgcca cactacgcgg gcctggaatg
       61 cgtttactca atgcacgaac cgtggtgaca agtatcgctt tttcgttatt aagcaattgc
      121 tcgatactct cctgccgagc cgctacgaaa gccggattgg cttcccggtc atcatagggt
      181 aaaagttcgt cttccgggaa ttgtaaaatc gggattttgt gggttgtata gtcttgcagc
      241 gttgtcagat catcgagtac acgaccatac gtcaaactgt ccggcacaac gataacggat
      301 aagcggtcat tcacataggc cgactgagcc gctaaaatcc caagcaccgc atccatcgaa
      361 ccgagatacc cgtccaccat gatcgtcttc ttttcggcga tatggcgaag taaattttgc
      421 gcagattcag aggacgttat acgctgaaga actatatcaa gcatgtatag agacttcgat
      481 tagatatatt cgccgaaaag cgtgtggccg tcggcacgtg caatacgaat acgagcatac
      541 tcaccgatgg ttttatcgga cttaggtatg atgataatct gtcctttgtc ggtacgcgcg
      601 caataataat tatccgattt gcggctaact tcttcgatta atacttcgcg ttctttaccg
      661 atctcacggt gtaaagattt taatccgttt tccttctgga cttcaataat ctgttgcaag
      721 cgtcgtgact tctccgcttc tgttacatca tcgggcattg tatacgcttt agtcccaata
      781 cgcgccgaat atttaaacat aaatgccgag tcaaattgta ccgtacgaaa cagttccaat
      841 gtatccaaat gatcacgttc ggtttccgat ggaaacccgg tgataatatc ggttgaaatg
      901 gacacatccg gaattatgcg gcgcactgta tcaataagtt taagatattc ttcgcgtgta
      961 tatgtacggt tcataagttc caaaatacga ttggaacccg attgcacagg aatatgaagc
     1021 tgtttgcaaa tattatcgcg ttgtgcgatg atgtgcagaa gatgttcggg gaaatccttt
     1081 ggatgcggag aagtaaaccg aatcctacgc accccgggca cttcactgac gcgattcatc
     1141 aactcagcga aatccttttg ttgcggatca cggtatgaat tgacattttg acccaataac
     1201 ccgatttctg tatagccttg cgctacagct ttgtgtactt cttcgactat actttcaacg
     1261 gaacgactgc gctcccggcc ccgcgtataa ggaaccacac aaaatgtgca aaaattatca
     1321 catccccgca tgatcgctat ccacgccgtg attttaccaa gtcgcaatgg ttcaacctga
     1381 tcatacacct ccgtgcgtga caaacgcgta tccatttgca gaggttttaa ttgaaaatca
     1441 cgatattcgg ataaactgaa gtcctcgcca tgttgggacg gcaatcgaat ctgcacaaga
     1501 ttttgcgaag cctgtttttt ttctatcaac tccgggattt tacgatacga atccggacct
     1561 aaaacgacat tgacatacgg gcgtttggcg tggatttctt cttcaagatg ttttgccata
     1621 caacccaaaa caccaatgat gagatgaggt ttctcttctt tgagtttctt aaatcgcgtt
     1681 aactggcccc acacacgctg ctcggcattc tcacgaatag cacatgtatt cataaaaatc
     1741 gcatcggccg tttcataatc atccaccaga tcataatcca tatcgcgcat aatgctggcg
     1801 accaactccg aatcatacac attcatctga caaccgtagg tttccaaata taaacgttta
     1861 cggttgcctt gcaggcgcga acgtacatcg gcaatggatt caggtgtatg aatctcttca
     1921 aaggctgtat cggacatggg attactcatt gatcagagag tccgtaaggc tgagtgcaat
     1981 tccagagagc atgacttccg gccccaaagt cgtaagatca ccggacagca tttccgtatc
     2041 cacattggca tataatgagc aagctgcccc tttttctatc gtaagaccgg aaagttttcc
     2101 attctgtacg ccgacaaaag ttacttcatt caatagttcg ctgacgacat aaccgctgca
     2161 aaaatgaagt tgcagatgcg cgttgggtgt atatacagtg ataagcgtcc ccttacgacg
     2221 gccaatcggg atctccggat atatttccaa tgtaagtttt cgtccctcgg tctgattttc
     2281 aagatacatt ctgtaatgat cgccaaaatc ctgtttctcc actcccaaac ttttggcaat
     2341 gcgttccaca tctaccggcg acaagttaaa tttatgttca tcttctgatt tggttttttt
     2401 tgttttgagc gcaacggaac gtgttttatc ggttttttta attaccgatt taacgcgttt
     2461 ggaaactgcg ggttgagttt tctttttctt tttcatgatt gtgtattttg tgtatctaca
     2521 attactgcgc ctaaatcgtt atctacggaa cggttttttc gcagaagttc agattctttt
     2581 aattcttgtt cgatttgtgg aaacgtataa cgtacatctc cgttattatt caatgttgtt
     2641 tcaccgttaa aatctctcaa catgctatcc aaagcctcct ggacatcgtg ttttttgaga
     2701 acttcttccc ctttggattt accattgata tgagttacaa cttcatccac ggttgcagag
     2761 gccgacggca tcgaaaacaa ataccgcata attcgtttcc gtacattcat cgcccgacgg
     2821 tttcgttttt gtttaattat agcaaacgaa cgaaacaacg gtataataaa gaatatcagc
     2881 gaaaaaacaa agggaaccca tcctaaaaac acgccgatcc atggggattc attcatccat
     2941 ggcatatcct cgagaggccg gtaatacgtt ttgagaataa gcacggaaaa agccaaatta
     3001 aaaatattca tgaaactgat aagcgcattg cgtccggtcg tattgcccgt cagttcataa
     3061 tcaggttcat attcatccca gtaatattca attgcaccat cctgcgcact ttctttcttt
     3121 cgtgtgatct
//
LOCUS       DAOQNQ010000350         3123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_15972_length_3123_cov_4.216754, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000350 DAOQNQ010000000
VERSION     DAOQNQ010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15972_length_3123_cov_4.216754"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PK754_14670"
     CDS             complement(<1..519)
                     /locus_tag="PK754_14670"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10793.1"
                     /translation="MKFKTICIFLMICCVPLLGQEEIRFQIKQVIPIKTPDGNNVTQI
                     NSVCVTPLREVYMTESSGHRVLRMNTEGQMLGLFGGLGWSGEMLDYPTGITSHDGLNL
                     YVADMNNQRIVRLNRRLEWVASIGGHNLDYSGQESVHSVAYPLSLTQSKQGDLFYISQ
                     ATNRVYKVNSVTG"
     gene            572..1315
                     /locus_tag="PK754_14675"
     CDS             572..1315
                     /locus_tag="PK754_14675"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB10794.1"
                     /translation="MLFRNFISLSKNISMLLLGLTLANTACTQSAKTTEKKKVNAEVA
                     VIETDFGNIIIKFYDKEAPKHTENFKKLARAGFYDTLTFHRVIPGFMIQAGDPNSKDN
                     DPYNDGRGGPGYTVPAEFNKKRFHKRGALAAARQADQVNPRKESSGSQFYIVQNGPVS
                     LDVLKQMEAQFKAAKPGFYFTNEQIDTYTTLGGTPFLDGEYTVYGEVVSGLDVIDKIA
                     MVQTDPRNNRPVQNVIMKKVSIQTMEIEE"
     gene            complement(1393..2328)
                     /gene="miaA"
                     /locus_tag="PK754_14680"
     CDS             complement(1393..2328)
                     /gene="miaA"
                     /locus_tag="PK754_14680"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNB10795.1"
                     /translation="MNDLNPNVLVIVGPTAIGKTALAVDLSEYFDIEIVSADSRQLYR
                     YMDICTAKPDNTQRLRVPHHFIDHINPDETYNAAQYAREALPVIQSIFDRGKQPVIVG
                     GSGLYIRALIDGFFEGVDNNEIVRAEIRKQIDSEGIVSVFEELRQNDPETANRLHISD
                     VQRIERALEVFRVTGKPLSQWHKETQSKFPWNSLWVGLDCDRTKLYQRIEMRVDQMIE
                     QGVIGETEMLLERGYSENLISMQSLGYREIIAYLKGTMDCDTMVRLFKQGSRNYAKRQ
                     LTWFRKNERIRWIMTDKNSPEKILDNTLKHFNLNK"
     gene            complement(2345..>3123)
                     /locus_tag="PK754_14685"
     CDS             complement(2345..>3123)
                     /locus_tag="PK754_14685"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HNB10796.1"
                     /translation="NDIRTSDGTAMMLELNTQRQPLQWTTPAHSNTAPQPTSDEAVAQ
                     FFSAEETAPSPKSYIQDQLVKRIPVNLNSMVWQLHNRYIVSQIKSGLAIIDQHVAHER
                     ILYERALDAFEHNPIFSQQLLFPQTIEFSPEDFSIIEKHIALMERLGFVVKVFGKRTL
                     VVEAVPADVRLGNEQKILTEIIREYKEHEHDAMDPRDNLAKSFACKSAIKSGDPLNVD
                     EMNGLIDQLFACKFPYVCPHGRPIIIQLTLDELDKRFMRT"
BASE COUNT          878 a          714 c          658 g          873 t
ORIGIN      
        1 gcccgtgacc gaattgactt tgtacacacg atttgtcgcc tgcgatatat aaaaaagatc
       61 gccctgtttg ctttgggtta gtgacaaagg ataagccacg ctatgaacgg attcttggcc
      121 tgagtaatcg agattatgcc cgccgatgga agccacccat tccaatcgcc tatttagccg
      181 gacaatccgc tgattgttca tatcagccac ataaagattg agtccatcat gagaggtaat
      241 ccctgtcggg taatccagca tttcaccact ccagcctaac ccaccaaaca aaccaagcat
      301 ttgaccttca gtattcattc gaaggacacg atgcccggac gattccgtca tatacacttc
      361 gcgcaaagga gtaacacata cggaattgat ctgtgtaaca ttgttaccgt ccggtgtttt
      421 gatgggaatt acctgtttaa tctgaaaacg tatttcctct tgcccaagaa gaggaacgca
      481 gcagatcatt aagaagatac aaatcgtttt gaatttcacg tttttttccg atagattggg
      541 gtcgcttaaa taattaataa aggaggttcc cttgctgttc cgtaatttca tatcgttgag
      601 taaaaacata tccatgctgt tattaggcct tacattagct aatacggctt gcacgcaatc
      661 ggccaaaacc actgagaaga agaaagtaaa tgcggaagtc gccgtgatcg aaactgattt
      721 tggcaatatt ataatcaaat tttacgataa agaagcgccg aaacacacag aaaattttaa
      781 aaaattggcc cgcgccggtt tttatgatac gctgacattt caccgtgtca tacccggatt
      841 tatgattcag gccggtgatc ccaattccaa agataatgac ccttataatg acggtcgcgg
      901 cggtccgggt tatacggtgc cggcagagtt taataaaaaa cgttttcaca aacgtggcgc
      961 attagccgct gcgcgtcaag ccgatcaggt caatccgcga aaagaatcca gcggttcaca
     1021 attttatatc gtacaaaacg gccctgtatc tttagatgta ctcaaacaga tggaagcgca
     1081 gtttaaagcg gccaaaccgg gtttctattt caccaatgaa caaattgaca cttacaccac
     1141 cttgggcggt acgcctttct tagacggcga atataccgtg tatggtgaag ttgtcagcgg
     1201 tcttgatgtc atagataaaa ttgctatggt acaaaccgac ccacgcaata atcgccctgt
     1261 tcagaatgta attatgaaaa aggtttccat acaaacgatg gagatcgaag agtaaaaatt
     1321 aaaacggcca ctccggtata tgttattgac atgacccggg tggccgttac ttatttaata
     1381 taaattttat tcttatttgt tcaggttgaa atgctttaat gtattgtcca atattttctc
     1441 cgggctattt ttatccgtca taatccatcg aatgcgttcg ttcttcctaa accatgtcaa
     1501 ttgacgttta gcgtaattac gcgatccttg tttaaataaa cgcaccattg tatcgcaatc
     1561 cattgtccct ttcagataag cgataatctc tctatatccc aaactttgca tcgagatcaa
     1621 attttccgaa tatccccgtt caagcagcat ctcggtttca ccgatcaccc cttgttcgat
     1681 catttgatcc acacgcattt caatacgttg atacagtttt gttcggtcgc aatccaatcc
     1741 gacccacagt gaattccaag gaaacttgga ttgggtttcc ttgtgccatt gggacagcgg
     1801 tttaccggtt acacgaaaaa cctccagtgc gcgctcaatt ctttgtacat cgctgatatg
     1861 caaccggttc gccgtctcgg ggtcattttg ccgtaactct tcaaaaactg atacaatacc
     1921 ttcagaatct atttgctttc gtatctcagc acgcacgatc tcgttattat ctacgccttc
     1981 aaaaaatcca tcaatcaaag cacgaatata caatcccgat cctcccacaa tcaccggttg
     2041 cttaccgcga tcaaagatgg attgaataac gggaagtgct tcacgcgcat attgcgcagc
     2101 attataggtt tcgtccgggt tgatatgatc aataaaatgg tgcgggacac gaaggcgttg
     2161 tgtgttatcc ggtttggccg tacaaatatc catatagcgg taaagctggc gggaatcggc
     2221 ggaaacgatt tcaatatcaa aatattcaga aaggtctaca gccaaagccg ttttaccgat
     2281 cgccgtcggt ccgacaatca ctaatacatt gggattcaaa tcgttcataa aagatgcgcc
     2341 ttgctcaggt gcgcataaaa cgtttgtcca attcatccaa tgtcaattgg atgataatag
     2401 gtcgcccgtg cggacatacg taagggaatt tgcaggcaaa taattggtct ataagtccgt
     2461 tcatttcatc cacgttcaaa ggatcgccag acttgatagc cgatttgcag gcaaatgatt
     2521 tggccagatt atcacgggga tccatcgcgt catgttcgtg ctctttgtat tcccgtataa
     2581 tctccgtaag gattttttgt tcattaccaa gacgaacatc ggcaggaacg gcttcgacga
     2641 ccagcgtccg tttaccgaat actttcacga caaagccgag ccgctccatc aaagcaatat
     2701 gtttttcaat aatggaaaaa tcttcaggag aaaactcaat cgtctgagga aaaagtaatt
     2761 gttgtgaaaa aatcggatta tgctcaaacg catccagcgc tctttcatag aggattcgct
     2821 catgcgccac atgctgatcg atgatagcca aaccgctttt gatttgcgaa acgatatacc
     2881 gattatgcag ctgccaaacc atactgttga ggttgaccgg gatacgtttg accaattgat
     2941 cttgtatata acttttgggg ctcggagccg tttcttccgc tgagaaaaat tgcgctacgg
     3001 cttcgtcgga ggtcggttgc ggtgcggtgt tggaatgcgc cggtgtcgtc cactgcaaag
     3061 gttgacgttg tgtatttagt tccagcatca tagccgtgcc atcggaggta cgaatatcat
     3121 tgg
//
LOCUS       DAOQNQ010000351         3115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_16042_length_3115_cov_4.724510, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000351 DAOQNQ010000000
VERSION     DAOQNQ010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16042_length_3115_cov_4.724510"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /gene="lepA"
                     /locus_tag="PK754_14690"
     CDS             <1..1310
                     /gene="lepA"
                     /locus_tag="PK754_14690"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNB10797.1"
                     /translation="KAGIGIEDILEKVVESIPAPVGDPTKPLQALIFDSVFDPFRGAI
                     AYVRVFQGELVTGTHMKFFSNDCRYDADEIGYLRMDKIKADKISVGEVGYVVGNVREI
                     THIRVGDTVTDAGNPAPAALEGYKDIKPMVFSGIYPTVSEDFEELRTSLEKLKLNDSS
                     LIYEPESSTALGFGFRCGFLGLLHMEIVQERLQREFNISMITTVPSVRYQVYKTNGEV
                     IEVSNPSMMPSPGIIDHIEEPYIKAQIITPTEYIGNIMSVAMERRGVYRNTNYISPTR
                     VDLTFEFPLAEIVFDFYDKLKSCTKGYASFDYEYIGYKEGELVKLDILINSDPVDAFS
                     GIIHRDKAYEFGRKMCEKLKELIPRQMFEVVIQAAIGSKIIARDQIRAMRKDVTAKCY
                     GGDISRKRKLLERQKEGKKRMKQVGKVEIPQEAFLAVLKIGDE"
     gene            1347..1700
                     /gene="cutA"
                     /locus_tag="PK754_14695"
     CDS             1347..1700
                     /gene="cutA"
                     /locus_tag="PK754_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNB10798.1"
                     /translation="MSNVTSNVTARTAIIVYMTASSDAEAKHIADALITEQLAACCNI
                     CPGIRSIYMWEGKLCDEKEVLLLIKSFEDCFEALAKKIKSLHSYKVPEIIAVPLTKGD
                     EAYLRWMEEVVPSKP"
     gene            complement(1690..2742)
                     /locus_tag="PK754_14700"
     CDS             complement(1690..2742)
                     /locus_tag="PK754_14700"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNB10799.1"
                     /translation="MNIIEREPWIQSFLQETDAVIHEVFENTGSAEFKDILKTHHQGG
                     KRLRPMVIAASAQLGDRPAHHLAYGAAALELLHLASLFHDDVLDDTTMRRDKLTARKH
                     IGNLNSILTGDYLLAEVLHTIVEKLPTPLVSAFLDTIKRMTRSEILSKKLLNNLQIST
                     SEYVQIIENKTATVFALATSLGIRLSTDDPVRIRQLSIYGMNLGMAYQIIDDMEDMTG
                     ASELTDDDLSHGYIGMPVIELLRAVPLNQKQEVLQMVRATQPEQRAALIRMMKSFSIF
                     KVVHARVQHYLNISANALLDIKANTPEQRISLEFLKGLCGYLADKSLKAVDTYESADH
                     TGTQTVSASTTVAATA"
     gene            complement(2759..>3115)
                     /locus_tag="PK754_14705"
     CDS             complement(2759..>3115)
                     /locus_tag="PK754_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10800.1"
                     /translation="ALKTWGVIRKVRVQGDRKDYYELDEDTGKIMTRFVRERKRRELE
                     PALETMRRSAILLRTAIPAMKNGERKQAQIFLKRIENMLMVSESINTLLERFIHGESI
                     SINQLKTIPIAWKKGA"
BASE COUNT          809 a          684 c          789 g          833 t
ORIGIN      
        1 ctaaagcggg tatcggtatc gaagatattc tcgaaaaagt cgtagagagt attcccgccc
       61 cggtcggcga tccgaccaaa ccattgcaag cgttgatttt tgattcggtg ttcgatcctt
      121 ttcgcggagc tatcgcgtat gtacgtgtgt ttcagggcga actggtcacc ggtacgcata
      181 tgaagttttt tagcaacgac tgccggtatg atgccgacga gatcggttac ctccggatgg
      241 acaaaatcaa agcggataaa atttctgtcg gtgaagtagg atatgtcgtc ggcaacgtgc
      301 gtgagattac gcacatccgc gtgggcgata cggtcaccga tgccggaaat cccgcaccgg
      361 cggctcttga gggatacaaa gatatcaagc cgatggtgtt tagcggtatt tatcctaccg
      421 taagcgaaga ttttgaagaa ctgcggactt ctttggaaaa actcaagctc aatgattcgt
      481 cgctgatcta cgaaccggag tcgtccacgg ctttgggttt tggtttccgt tgcggtttct
      541 tgggtctttt gcatatggag attgtgcaag aacgcctgca acgcgaattt aacatcagca
      601 tgatcacgac ggtacccagc gtgcgttatc aggtgtataa aacgaacggg gaagtgatcg
      661 aagtgtctaa tccttcgatg atgccttcgc ccggaattat tgatcacatc gaagagccgt
      721 atatcaaagc acaaatcatc acgccgacgg aatatatcgg taatatcatg agcgtcgcga
      781 tggaacgccg gggtgtgtac cgtaatacca attatatttc tccgacccgc gtggatctga
      841 cgtttgagtt tcctttagcc gagatcgtat ttgattttta tgataaacta aaatcctgta
      901 ccaaagggta tgcctcgttt gattacgaat acatagggta taaagaaggc gaattggtca
      961 aactggatat tctgatcaac agcgatccgg tggatgcgtt ttccggtatc attcaccgtg
     1021 ataaagctta cgagtttgga cgcaaaatgt gcgaaaaact taaggaattg atcccccgcc
     1081 aaatgtttga agtggtgatt caggctgcga tcggcagtaa aatcattgcg cgcgaccaga
     1141 tacgcgcaat gcgtaaagat gtgacggcca agtgttacgg cggtgatatt tcccgtaaac
     1201 gcaaactgct tgagcgccag aaagaaggta aaaaacgtat gaaacaagtc ggaaaagtgg
     1261 aaattcctca agaagcgttt cttgccgtcc taaaaatcgg cgatgaataa tccacaattt
     1321 attcgattat acaacgcgtt catactatga gtaatgttac gagtaatgta acggctcgca
     1381 cggcgatcat cgtatatatg acggcatcgt cggacgcaga ggcaaagcat atagccgatg
     1441 cgcttatcac ggagcaactg gcggcgtgtt gtaatatatg tcccgggatt cgatcaatat
     1501 atatgtggga aggaaaactt tgcgacgaaa aggaagtttt acttttaatc aaatcgtttg
     1561 aagattgttt cgaagcgtta gccaaaaaaa taaaatcact gcacagctac aaggttcctg
     1621 aaattatcgc cgttcctctg acgaaaggcg atgaggccta tctgcgatgg atggaggagg
     1681 ttgttccttc taagccgtag ccgctacggt tgtcgaggcc gaaactgttt gtgtgccggt
     1741 gtgatccgct gactcgtacg tatccacagc ctttagtgat ttatcggcca agtatccgca
     1801 caagcctttc aaaaattcca aagaaatccg ttgctccggc gtgttggctt taatatccaa
     1861 aagcgcattg gccgaaatgt tgaggtaatg ttgtacgcgg gcatgcacga ctttaaaaat
     1921 ggaaaaactt ttcatcatac gaatcaacgc agcacgttgt tcgggctggg tggcgcgtac
     1981 catttgcagc acctcttgtt tttgattgag gggcaccgca cgcagcaact cgatcaccgg
     2041 catgccgata taaccgtgag aaagatcatc atcggttaat tccgaagcgc cggtcatatc
     2101 ttccatgtcg tcaatgatct gataggccat accgaggttc atcccgtaga tactgagctg
     2161 acgaatgcgc accgggtcat cggtgctcag gcgaataccg agagaagtcg ccaaagcaaa
     2221 aaccgttgca gttttgttct caataatctg aacgtattca gacgtactga tctgcaaatt
     2281 gttcagaagt tttttggata aaatttcact tcgtgtcata cgtttgatcg tatcaagaaa
     2341 cgcgctgaca aggggcgtag gtaatttttc gacgatcgta tgcaaaactt cggccagcag
     2401 gtaatcgccg gttaagatcg aattgagatt tccgatatgt ttacgcgcgg tcagtttatc
     2461 gcgacgcatg gtggtatcgt caagcacgtc gtcgtgaaat aatgaagcta agtggagcaa
     2521 ttcgagtgca gccgcgccgt aagcgagatg gtgcgccggg cgatcaccga gttgagcgga
     2581 agccgcaatg accatcggcc gaaggcgttt gccgccctga tgatgggttt tgaggatatc
     2641 cttaaattca gcgctacccg tattttcaaa aacttcgtgg atgaccgcgt cggtttcttg
     2701 taaaaaactt tgtatccacg gttcgcgttc gatgatattc atacgacacc tttatgtgtt
     2761 atgcgccttt tttccaagct atgggtatgg tttttaattg gttgatgctg atggactcgc
     2821 catgaataaa gcgttccagc agggtattga tgctttcgga gaccataagc atgttttcga
     2881 tacgcttgag aaagatctgc gcctgcttgc gttctccgtt tttcatagcg ggaatggccg
     2941 tgcgaagcaa tatggcgctg cgacgcatcg tttctaatgc cggttcgagt tcgcgtcgtt
     3001 ttcgctcgcg cacaaaccgg gtcataattt tacccgtatc ttcatccaat tcgtaataat
     3061 ctttacggtc gccttgcacc cttactttgc ggataacgcc ccatgttttg agcgc
//
LOCUS       DAOQNQ010000352         3110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_16088_length_3110_cov_3.449755, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000352 DAOQNQ010000000
VERSION     DAOQNQ010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16088_length_3110_cov_3.449755"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PK754_14710"
     CDS             complement(<1..224)
                     /locus_tag="PK754_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10801.1"
                     /translation="MLDDIFTPSDEQDITLTNDERTRIEGILSKLVTESAQQGVSIVA
                     LKNHKISKPILFKLAKDGNSNASYVLSELNL"
     gene            complement(228..692)
                     /locus_tag="PK754_14715"
     CDS             complement(228..692)
                     /locus_tag="PK754_14715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB10802.1"
                     /translation="MHTIIEDAIKNMSVLASQKEILLINEFENTDYKAYFDGEKILQV
                     FNNLISNAIKFTSTGGAVMIGAQPVPDGVQFMVKDTGIGIPEEDLPHIFEKYKRTGGV
                     FSSDDHAGGGGIGLGLAICKSIIEAHKGRIWAESAVGEGTTFYFTVLTPEQS"
     gene            complement(745..1914)
                     /locus_tag="PK754_14720"
     CDS             complement(745..1914)
                     /locus_tag="PK754_14720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB10803.1"
                     /translation="MAKEKILVVDDSATQLIMYKMALSKAGYLVISAKNGVEGMHMVE
                     TENPDLIVSDIIMPELNGYQFCRLVKDDPSRAHIPIILLTSLGQQQDRFWGIEAGANA
                     FVTKATDTTALLQAVEELIKTIAKPSTIDYADKEDEKYSIQSPENPEESIKSKLNRIL
                     EKLLYETTVSNRMRDLSRYAYNRQEMITRCFDLFRQIVEYHASALTLFEKDTLHLTID
                     IQKDIADNFLDKAIASVLQQDFNPNEVRMGLIKNTQKDIFGETHLKPEGAQDVQSVLV
                     VPLRDETEVIGSLAVFTSDTFAYNKDIYRLIQLIARELTLTIKYIWKLEELENIKRNF
                     TSTIVNDLRSPIIANQSFAEALFNEYVDPITEDQREILSNVIANNKKLLTFKRHH"
     gene            complement(1924..3096)
                     /gene="cheB"
                     /locus_tag="PK754_14725"
     CDS             complement(1924..3096)
                     /gene="cheB"
                     /locus_tag="PK754_14725"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HNB10804.1"
                     /translation="MPKILIAEDSPTVTMILQKIFATDDDCQVIGVAKNGREAVEKVQ
                     ILRPDIVTMDIRMPVMDGFEATKQIMQTSPTPILVISASVGKDDLNIAFNAIRAGALD
                     IVEKPKGNLAMDYEYIGRDLIKKVKILSGVKVFHHIGGAKNKSSDAKSNDKTDTLSVR
                     QPRVPAPAAEVPSVKETLWREPLTPKSVYPKLLSPVKTPEVIAIASSTGGPSALLKLL
                     KTLPAGFPVPVVIVQHICEGFGQGFVEWLNKECGMNVKTADRGEELQPGTIYVAPDGY
                     HLFIDSGKIVRLSKSMPVNGLRPNATLMMESVAKVFGAASIGIVLTGMGRDGADGAKA
                     IKEAGGFTMAQNAESCVVFGMPKETIELGVVDKILPPEKMTAELMAFFKLYQTANA"
BASE COUNT          782 a          735 c          665 g          928 t
ORIGIN      
        1 agattgagtt ccgataaaac ataactggca ttgctgttac cgtctttagc cagtttaaac
       61 aaaataggtt tagatatttt gtgatttttc aatgctacga tactcacgcc ttgctgcgcg
      121 ctttccgtca ccagtttgga taatatcccc tcaatacggg tacgctcgtc attggtcaac
      181 gtgatatctt gttcgtcgga cggtgtaaaa atatcgtcta gcatacgtta tgattgttcc
      241 ggtgttaata cggtgaaata aaatgtggta ccttcaccga ccgcactttc agcccaaatg
      301 cggcctttat gcgcttcgat gatggattta caaatggcaa gtcccaaccc gatgccgccg
      361 ccgccggcat gatcgtcgct ggaaaacaca ccaccggttc gtttgtattt ttcaaaaata
      421 tgcggcaagt cttcctccgg aatgccgatt cctgtatctt taaccataaa ctgtacgcca
      481 tcaggcaccg gttgtgctcc gatcatgact gctccgccgg tggaggtaaa tttgatagca
      541 ttggagatca ggttattgaa tacctgtaat attttttcgc cgtcaaaata cgccttgtaa
      601 tccgtatttt caaattcatt gataagtaaa atttcttttt gggaagccaa gacagacatg
      661 ttttttatgg cgtcttcgat gatcgtatgc acattattgt gttccgggaa aatttccagt
      721 ttgccggatt caattttcga aatgtcaatg atgtcgtttg aacgtcagta attttttatt
      781 attcgcaatg acgttggaca agatttctct ctgatcttcg gtgatcggat cgacgtattc
      841 gttaaacagc gcttcggcaa agctctgatt ggcgatgatc ggacttcgca gatcattgac
      901 gatcgtcgat gtaaagttac gtttgatatt ttcgagttct tccaatttcc aaatatattt
      961 gatcgtcaac gtcagttcac gcgcgatcaa ctggatcagt cggtaaatat ctttgttgta
     1021 cgcaaatgta tccgatgtaa aaacagccaa ggaaccgatt acttccgttt catcccgcaa
     1081 aggcacaacc agcacggatt ggacgtcctg tgcgccttcc ggcttaaggt gggtttcacc
     1141 aaaaatatct ttctgggtat ttttgatcag tcccatacgc acttcgttgg gattaaaatc
     1201 ttgctgcaat accgatgcga tggctttatc caaaaaatta tcggcgatgt ctttttgaat
     1261 atcaatcgtc aaatgcagcg tgtctttctc aaaaagcgtg agtgcagatg catgatattc
     1321 gacgatctgt cgaaagagat caaaacagcg cgtaatcatt tcctgacgat tgtaagcata
     1381 ccgcgacaag tcgcgcattc ggttagatac cgtcgtctca taaagaagtt tctctaagat
     1441 gcgattcagt ttggatttga tactctcttc cgggttctcc ggcgattgaa tcgaatattt
     1501 ttcatcttct ttgtccgcat aatctatggt tgaaggcttt gcgatcgttt tgattaattc
     1561 ctctaccgct tgcagcagcg ctgtcgtatc cgttgctttg gtaacaaacg cattggcacc
     1621 ggcttcaata ccccagaaac gatcctgctg ctgaccgaga cttgtgagca aaataatcgg
     1681 aatgtgcgcg cgggaaggat cgtctttgac caatcggcag aattgatatc cgttgagttc
     1741 cggcatgatg atgtcgctca cgatcagatc gggattttcc gtctccacca tgtgcatgcc
     1801 ttcgacgccg tttttggcgg agatgacgag atatcccgct ttgctcaacg ccattttata
     1861 catgatgagc tgcgtcgcgc tgtcatctac aaccaatatt ttttctttag ccacaactac
     1921 ctcttacgcg ttagccgttt gatataattt gaaaaaagcc atcagctcgg ctgtcatttt
     1981 ttcagggggt aaaattttat ctacaacacc caactcaatc gtttctttgg gcataccaaa
     2041 tacaacacaa gattcggcat tttgtgccat tgtaaaacct ccggcttctt tgatggcttt
     2101 ggctccatca gcgccatcac gtcccatgcc agtcagaact ataccgatcg aagccgcacc
     2161 aaaaacttta gcaaccgatt ccatcatcag cgtcgcattg gggcgaagac cattcaccgg
     2221 catggatttg ctgaggcgaa cgattttgcc tgaatcaata aaaagatgat agccgtccgg
     2281 agcaacataa atcgttcccg gttgtaattc ttcaccgcga tccgctgttt ttacattcat
     2341 accgcactct ttattgagcc attcaacaaa tccttgcccg aatccttcac aaatatgctg
     2401 gacaataact acaggaaccg gaaatcctgc cgggagggtt ttgagcaatt tgagtaacgc
     2461 cgatggtccg cccgtcgatg aagcgatagc tatcacctcg ggtgttttta cgggactcaa
     2521 tagtttcgga tatacgctct tgggcgttaa aggttcgcgc cagagtgttt ctttgactga
     2581 cggcacttcg gcagccgggg caggcacgcg cggctgacga acggataatg tgtcggtttt
     2641 atcattagat ttcgcgtccg acgatttatt tttagcgccg ccgatatgat gaaaaacttt
     2701 gacaccggaa agtattttaa cttttttgat caaatcgcga ccaatatatt cgtaatccat
     2761 cgccagattg cctttgggct tttcgacaat atccaacgct ccggcacgaa tggcattaaa
     2821 cgcaatattg aggtcatctt tacccacaga cgcactgatc actaaaatcg gcgttggtga
     2881 tgtttgcatg atctgttttg tcgcttcaaa tccgtccata accggcatac gaatatccat
     2941 cgtcactata tccggccgga gaatctgaac tttttcaacc gcttctctgc cgtttttagc
     3001 cacgccgatt acctggcaat catcgtccgt cgcaaaaatt ttctgaagaa tcatcgtgac
     3061 ggtcggcgaa tcttcagcga tgagtatctt gggcatcgac tcctacaata
//
LOCUS       DAOQNQ010000353         3078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_16415_length_3078_cov_5.178300, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000353 DAOQNQ010000000
VERSION     DAOQNQ010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16415_length_3078_cov_5.178300"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1922
                     /locus_tag="PK754_14730"
     CDS             <1..1922
                     /locus_tag="PK754_14730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB10805.1"
                     /translation="QQAQMVDNRSVRNMMRSKRNPNQVWVGLSDGLALCRIDKEQWVR
                     VEEHRTIKEEVRTIAESLNGDLWLGTRFKGVIHLKREGDSLNWPVTRYDRANGLPIGE
                     MYMYEVDGRPLVGTTSGLFRYDVDHNHFVRETLFGPLLSDSATVVHHFLQHPQFGLWM
                     TAQVGERFEILHAASSSRAISYTVRNPFLRISDFRVNIPYPESGNIIWFGGADGLIRY
                     DGNIQYAWDSQFYALIRKAVVMNDQRQNIPILSDTSQHVMLAHYDNNIRFEFSASSYE
                     RDGSNQFQYYLEGFDPGWSEWSTEFRKDYTNLPDGVFRFRVRARNLYEQVSVEDQLRF
                     VIRSPWFKSWWAFGFYTALVFLCIYVIIHYRERRLVLANDNLERIIQQRTAELKQKNV
                     ELENLNEKKNEYLGIVAHDLRSPLTTIMGYIDLLIADAETDTFKPKEAVKDLVRISRI
                     SHQMSHFIRELLDISAIESGKIRLEVLPDNLGTIIRDAEPLHRRAANQKNIAMDYEYD
                     PTLPDTLMDREKIKSVVDNLINNAIKYTYPGGKVRVYHEVRDQDVVTHVEDTGQGLSD
                     EDKQTVFMSFKRLSAKPTAGESSTGLGLAIVKKIIEIHNGQTLVRSNKGQGSTFSFSL
                     PRADKQSDKNLKVKV"
     gene            1928..>3078
                     /locus_tag="PK754_14735"
     CDS             1928..>3078
                     /locus_tag="PK754_14735"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB10806.1"
                     /translation="MQYQKFFRSIGVMLVFVGMGAPHDVFSQQINMKFEHLSGDQGLS
                     QSTVGVILQDHTGLMWFGSRNGLNQYDGYRFTVYKSSPTDPESISDNWITALYEDRNH
                     NFWVGTLNKGLNRFDRARKKFKHMISDPENQRTISHNSITAIMEDRHGNIWVGTKNGL
                     NVIPYDSMRTETTMRFVRYFSSSQVVNSISHSWITAITDDENGDIWIGTQQGLNKITN
                     GEIIRYFADKQGGSGLPSNNITALFKDANNNIWIGTEDGGLCKLDRKNTQNPVFEKIP
                     VHSGTDASTTHADIVTVMVDRQNIVWVGTRLGLDRIDFNSNTKLFFRHNPNDVSSLSD
                     NEIRALCEDRSGNLWIGTYGGGGLNKLDLKPVKFNHIRHKPDNPNSLIN"
BASE COUNT          859 a          615 c          762 g          842 t
ORIGIN      
        1 gtcagcaggc acaaatggtg gataatcgtt cggtgcgaaa tatgatgcga tccaaacgaa
       61 acccaaatca ggtgtgggtt ggtttgtccg acggattagc gttgtgccgg attgataaag
      121 aacaatgggt gcgtgtcgag gaacatcgta cgatcaaaga ggaagttcga acgatcgccg
      181 agtctttgaa tggtgatttg tggctcggta cgcgtttcaa aggcgtcatt cacctcaaac
      241 gcgaagggga ttcactcaac tggccggtta cccgttatga ccgagctaac ggtttaccca
      301 tcggcgaaat gtatatgtat gaagtggacg ggagaccctt ggtcgggacg acatcagggc
      361 tttttcgtta cgatgtggat cataatcact ttgtgcgaga aactcttttt ggtccgttac
      421 tttcggacag cgcaacagta gttcatcact ttttgcagca tccgcagttc ggtttgtgga
      481 tgaccgctca agtcggagaa cgatttgaaa ttttgcatgc tgcctcgtca tcgcgcgcga
      541 tttcttatac cgtaagaaac ccgtttctgc gaatatcgga ctttcgcgtc aatataccgt
      601 atccggaatc gggcaacatc atctggtttg gcggcgcgga cggacttatc cgttatgacg
      661 gcaatatcca atatgcttgg gattcgcagt tttatgcact gatacgcaaa gccgtggtga
      721 tgaatgatca gagacagaat attccgattt tatcggatac atcacagcat gttatgttgg
      781 cgcactatga caataatatc cgatttgaat tttcggctag cagctacgaa cgcgacggat
      841 ccaatcaatt tcaatactat ctcgaaggtt ttgacccggg ttggtcggag tggtcaacgg
      901 agtttcgtaa agattatacc aatttgccgg acggagtttt tcgatttagg gtgcgtgcac
      961 gcaatctcta cgaacaagtc agtgtcgaag atcaattgcg gttcgtcata cgatcgccgt
     1021 ggttcaaatc atggtgggcg tttggctttt atacggcctt agttttctta tgtatttatg
     1081 tgataattca ttatcgtgag cggcgattgg tacttgccaa tgacaatctc gaacgcatca
     1141 ttcagcagcg gaccgcggaa ttgaaacaaa aaaatgtgga attagaaaat ctgaacgaaa
     1201 aaaagaacga atatttgggt attgtagcac acgatttgcg tagtcctttg acaacgatta
     1261 tgggatatat tgatcttttg atcgccgatg cggaaacgga tacattcaaa ccgaaagaag
     1321 ccgtaaaaga tctagtccgt atttcccgca tatcgcatca gatgtcgcat ttcatacgag
     1381 aattgctgga catctcggct attgagtcgg gcaaaatccg gttagaagtt ttgccggaca
     1441 atctgggtac tattattcgc gatgcggaac cgttgcatcg gcgtgccgcg aatcaaaaaa
     1501 atatcgccat ggattacgaa tatgatccga ctttaccgga tacattgatg gatcgtgaaa
     1561 aaataaaatc ggttgtggat aatcttatca ataatgcgat caaatatacg tatcccggcg
     1621 gtaaagtgcg tgtgtatcat gaagtgcgtg atcaggatgt ggtaacgcac gttgaagata
     1681 ccggtcaagg ccttagcgac gaagataaac aaaccgtatt tatgagcttt aaacgcctga
     1741 gcgccaaacc cacggccgga gaatccagta caggcttggg gctggctatc gtaaagaaaa
     1801 ttatcgaaat tcataatggt cagaccttgg ttcgcagcaa caaagggcaa ggttcgacct
     1861 tcagttttag tttgccgcgg gcggacaaac aatcggataa aaatctaaag gttaaggttt
     1921 aacctatatg cagtaccaaa agttttttcg aagcatcggc gtgatgttgg ttttcgttgg
     1981 gatgggcgct ccgcatgatg ttttttcgca acagataaat atgaagtttg agcatctgtc
     2041 cggcgatcag ggtttatcgc agagcactgt cggcgtgatt ttgcaagatc acaccggttt
     2101 gatgtggttc ggctcgcgca atggtctcaa ccaatatgac ggatatcgtt ttacggtgta
     2161 caaatcaagt cctacggatc cggaatctat ttccgataat tggatcacgg cattgtatga
     2221 agatcgaaat cataattttt gggtcggtac cttaaataaa ggtttgaatc gttttgatcg
     2281 ggctcgaaaa aaatttaagc acatgatttc ggatcctgaa aatcaacgta cgataagtca
     2341 taattcgatt acggcgatta tggaggaccg gcatggcaat atttgggtgg gcaccaaaaa
     2401 tggcctcaac gtcattcctt acgattcgat gcgaaccgag actacaatgc ggtttgtgcg
     2461 gtattttagc agcagtcaag tagtcaattc catttcacat tcgtggatta cagctatcac
     2521 ggatgatgag aatggcgata tctggatcgg tacgcagcag ggacttaata aaataaccaa
     2581 cggtgaaata atacgttact ttgccgataa gcagggcggt tcgggactcc catccaataa
     2641 tattacagct ttgtttaaag acgctaataa taatatttgg atcggtacgg aagacggtgg
     2701 gctttgcaaa ttagaccgca agaacactca gaatcctgtt tttgaaaaaa tacctgttca
     2761 ttccggtact gatgcctcta caacacatgc cgatattgta acggtgatgg tggatcggca
     2821 aaatatcgtg tgggtcggta ctcgtttggg attggatcgg atagatttta acagtaatac
     2881 caaattattt tttcgacaca accctaacga tgtgtcgtcg ttgtccgata atgaaatccg
     2941 agcgttatgt gaagaccggt cgggcaatct gtggatcggt acttacggcg gcggcggctt
     3001 gaacaaactc gatttaaagc cggtcaaatt taatcacata cgtcataaac cggataatcc
     3061 caacagcttg atcaataa
//
LOCUS       DAOQNQ010000354         3077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_16427_length_3077_cov_7.337525, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000354 DAOQNQ010000000
VERSION     DAOQNQ010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16427_length_3077_cov_7.337525"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2063
                     /locus_tag="PK754_14740"
     CDS             <1..2063
                     /locus_tag="PK754_14740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNB10807.1"
                     /translation="AVDTSGNASDYSDEVSSSPYDQTAPSVPQNLTATAGDGQVTLNW
                     TANAENDLARYRVYMNGTQIDSTTNNSYTKNDLTNYTSYDFSISAVDTGKNVSAQTTT
                     ENATPYDQTAPAVPQDLAATAGDGQVTLNWTANGEGDFAHYRVYMDGTQIDSTTNNSY
                     TKTGLSNYTTYNFKLSAVDTSRNASALTSQVSSMPYDQTAPAVPQDLAATAGDGQVTL
                     NWTANSESDLARYRVYMDGTQIDSTTNNSYTKNGLTNDVTYNFKIAAVDTGLNASAFS
                     TQVSGTPTDMTAPSKPNVLAAVAGNTSIILTWRKNIESDVIRYRIYRGTTAAPTTQVD
                     STTSASDTTKTYSGLTNGTTYFFRVTAVDGHGNFSVYSDDISAKPLDAPGTLTVSLFQ
                     NKAAKKYAQITVISSKALSAAPTVTAKLGVNNTPVTMTAIASSNQQFRGGFVINSAGS
                     YTLQTSAQTLNGRDTTVQRTFNAVIAKSGEAATLASTDGKASLKLNRATLAEEAVLLS
                     EQSDDGVYTFSADAEANGSMVLEIQFDAAQYADPSKLFIYRMEKETWSKQKTQVFTKT
                     NTLKAQVESFGTYKIAYDGSFDGSNIVPTEFALLQNYPNPFNPSTTIRYDLREDETVS
                     LKIYNMLGQEVRTLRKGFQMAGEYRVQWDGRNNAGQVVSSGVYIYRLETKSYRATKKM
                     MFLK"
     gene            2082..2702
                     /locus_tag="PK754_14745"
     CDS             2082..2702
                     /locus_tag="PK754_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10808.1"
                     /translation="MKRFILKNLTLVTVIASLTACGGGGKGKSAYTKLVDQGESQFAT
                     GSFADARTSFNAAKLKNPNSATAYIGAAWCSMSLNDIAAALADFQSGLSKTNINADLY
                     AGYAFALNANGDFALSNQRIDEALNADTDWELSYDGSLNVNDLRLAKAENHFLLGQYT
                     ESLAQVKLLNTSFNADVNTVSGRAALSVEIERLRNALGKNIARIPA"
     gene            2761..2949
                     /locus_tag="PK754_14750"
     CDS             2761..2949
                     /locus_tag="PK754_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNB10809.1"
                     /translation="MSSANAFDNLKVNESFELVNDHDPKPLYYQFTHERAGLFGWEYL
                     EEGPETWRVRITRVQTGD"
BASE COUNT          908 a          694 c          705 g          770 t
ORIGIN      
        1 tggccgtaga tacatccggc aatgcatcgg attattcgga tgaagtatct tcatcaccct
       61 atgaccagac ggcaccttcg gtaccacaga accttaccgc aacagccgga gatggtcaag
      121 ttacgttgaa ctggacggca aacgctgaaa acgacttagc acgttatcgt gtttatatga
      181 atggtacaca gattgattcg actacgaata atagctatac aaaaaacgat ttaacgaact
      241 acacaagtta tgatttttca attagtgctg ttgatacggg taaaaatgta agtgcgcaaa
      301 ccacaacgga aaacgcaaca ccttacgatc agacagcgcc tgcggttcct caggacttgg
      361 ctgcaacggc tggagatggt caggttacat tgaactggac agcaaacggt gaaggtgatt
      421 ttgcacatta tcgtgtttat atggatggta cacagattga ttcgacgacg aacaacagtt
      481 atacaaagac cggtttatcg aactacacga catataactt caagttatcc gccgttgata
      541 ccagccgcaa tgcaagcgca ttgacttcac aagtatcatc tatgccctat gatcagacgg
      601 cccctgcagt tcctcaggac ttggctgcga cggctggtga tggtcaggtt acattgaact
      661 ggacggcaaa cagtgaaagc gacttagcac gctatcgtgt ttatatggat ggtacacaga
      721 ttgattcgac gacgaacaat agctatacaa aaaacggttt aactaatgat gtaacttata
      781 atttcaaaat tgctgccgta gataccgggc ttaacgctag tgccttctcc acgcaggtat
      841 cgggtacacc gaccgatatg acagctcctt caaaaccgaa tgtattagca gccgtagccg
      901 gtaatactag tattattttg acatggcgga aaaacatcga atcggatgta attcgttatc
      961 gtatataccg tggtacgacg gccgcaccga caacacaggt agattccacg acctctgctt
     1021 cggatacgac aaagacctac agcggtttga ccaacgggac gacgtacttt ttccgggtca
     1081 ctgctgttga cggacatggt aatttcagtg tgtattccga tgatatttca gctaaacctc
     1141 tagatgcgcc gggtacactc acggtaagtt tattccaaaa caaagcagct aaaaaatatg
     1201 ctcaaatcac ggtaatatca tccaaagctt tatcagcggc tcctaccgtg acggccaaac
     1261 ttggagtaaa taatacaccc gtcacgatga cggctatcgc gtcaagcaac caacagttcc
     1321 gcggtggctt tgtgataaat agtgcaggta gctatacgtt acaaacgtcc gcacagacat
     1381 tgaatggtcg cgatacgacc gtgcagcgca cgtttaatgc ggtgattgcc aaatccggtg
     1441 aagcggctac ccttgctagc acggatggca aagcttccct caaacttaat cgcgcgacgc
     1501 ttgctgaaga agctgtactt ctgtccgaac agtccgacga cggtgtttat acattctcag
     1561 cggatgcgga agccaacggc agcatggtac ttgaaataca gtttgatgcg gctcagtacg
     1621 ccgatccgtc gaagttgttc atttatcgaa tggaaaaaga aacctggagt aaacaaaaaa
     1681 cacaggtatt taccaaaacc aatacgctca aagcgcaggt agaaagtttt ggaacataca
     1741 agatcgcata tgacggttcg tttgacggat cgaatattgt gccgacagag tttgcgttgc
     1801 ttcaaaacta tccgaatccg tttaacccct ccactacgat ccgatacgat ctacgtgaag
     1861 atgaaacggt atctctgaaa atatacaaca tgctcggcca ggaagttcgt acgctgcgca
     1921 aaggattcca aatggccgga gagtatcgtg tacaatggga tggtcgcaat aatgccggac
     1981 aagtcgtatc cagcggcgta tatatctatc gcttggagac gaagtcctat cgcgcaacga
     2041 aaaaaatgat gtttttgaaa taaccgacga ggaaacaaaa aatgaaacgc tttatcctca
     2101 aaaatttgac gttagttacc gttattgcat ctctgactgc atgcggtggc ggaggtaagg
     2161 ggaaatcggc ttatactaaa ctagtcgatc agggtgaatc gcaatttgca accggcagtt
     2221 ttgccgacgc acggacttca tttaatgcgg ccaaattgaa aaaccccaat tcagcgacag
     2281 cctatatcgg tgccgcatgg tgttcgatgt cgctcaatga tatcgccgca gcattggccg
     2341 attttcaatc cggattatcc aaaaccaata ttaatgcaga tctttatgcg ggttatgcat
     2401 ttgctctcaa cgcaaacggc gattttgctt tgtccaatca acgtattgat gaagcattga
     2461 atgccgatac cgactgggag ttgtcctatg atggcagttt gaacgtaaat gatttgcgtt
     2521 tggcaaaagc agaaaatcat tttcttttag gccaatacac cgaatcgctt gcacaggtca
     2581 aacttcttaa cacgtcattc aacgcggatg taaacacggt ttctggccgc gcggcgttgt
     2641 ctgtggaaat cgaacgcttg cgtaatgcct taggaaaaaa catcgcacgc atacccgctt
     2701 aaaaaaatta aaccggattt ttataaagcc gtttcaggtt tacttgaaac ggctttttta
     2761 ttgtcatcgg caaatgcctt tgataatctt aaggtgaacg agtcgtttga actggtcaat
     2821 gatcacgacc ccaaacctct gtattaccag tttacacacg aacgggcagg tttgtttggg
     2881 tgggaatatc tcgaagaggg acctgaaacg tggcgggttc gcattacacg tgtccagact
     2941 ggagactaag catatgaccg attctgtaac tacagactat acggtgatta ataatctggt
     3001 gggattgcta gagaaaatcc aacccgacag catcaccagc cggacatttt ataaaagtga
     3061 tcgcttgaaa gccatcg
//
LOCUS       DAOQNQ010000355         3018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_17078_length_3018_cov_5.181235, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000355 DAOQNQ010000000
VERSION     DAOQNQ010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17078_length_3018_cov_5.181235"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PK754_14755"
     CDS             complement(<1..196)
                     /locus_tag="PK754_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNB10810.1"
                     /translation="MHKALHKSQELFTATWGEISTFWGINKVMGQLYALLFASAKPLT
                     LDDMCERLQISRGNASMNIRA"
     gene            364..1488
                     /gene="ribD"
                     /locus_tag="PK754_14760"
     CDS             364..1488
                     /gene="ribD"
                     /locus_tag="PK754_14760"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNB10811.1"
                     /translation="MPSPKTHEYYIRQTLKLARKGVGKTKTNPLVGAILVRHTPNGDQ
                     IIGRGYHTGYGKPHAEVEAVRDAQRRGVTDFKNTILYVNLEPCCHTGKTPPCTSLILQ
                     EKIPRVVFGIKDPFPAVAGKGAALLKKNGVRVEWGIAESECRIENRFFLKHVETGLPW
                     ITIKTAQTLDGKTADGFGASKWITNEKSRREVHRLRAGHDAILVGAQTVMADDPQLTV
                     RGVHGSSPRRVIVDGRLRSPLTARVFTDAGASETIVITTLGANKHKKRQLASLGVTVV
                     EMSSVRNRIAMRSIFRTLLRKFQVASVFVEGGADIHGQILNAGLCDEIHVFTAAKILG
                     HGKSSFDGLRVKRINNAVVLAEPRVRILDSDIWISARWQR"
     gene            1461..2141
                     /locus_tag="PK754_14765"
     CDS             1461..2141
                     /locus_tag="PK754_14765"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNB10812.1"
                     /translation="MDQRTVAAMMTMGNIITASLWGLGIGFINSIPFGPINMTIVDQS
                     FRRGFQPAMMIGLGALVVDIGYCIIGVFGMSALQQSLIDMLQPAGMPVLVFLGYRLII
                     TRHNGNVYHERTEFSAGLLMKNFINGCIMYLCNPLAIGFWIAAAGFMYAYQFLQSSLQ
                     SQLAFIAGMAIGTAAWFFLLAEWVAVRRQTMKETTIRRIAVITGWVLIVSGFILGWRY
                     VSYKGWIA"
     gene            2138..2785
                     /locus_tag="PK754_14770"
     CDS             2138..2785
                     /locus_tag="PK754_14770"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNB10813.1"
                     /translation="MIKIEVMFFDLDGTLVDSLDDIGASVNHTLTTLGYSALPKEKLR
                     RFVGDGMQTLLARALSEFEKPDNDQLMTAVSIYRTHHDEHCLDFVRTYDGVLDVLTHF
                     NHCRKFVISNKAEVFTRKIIHGLGLSRYFEGVYGGGDSFANKKPHPQAIQAMLEKTGV
                     HPDRAMIIGDGPQDIEAGRRAGVYTCAVRYGFHAVAPEGANFYIDRMIQLKEFVA"
     gene            2827..>3018
                     /locus_tag="PK754_14775"
     CDS             2827..>3018
                     /locus_tag="PK754_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNB10814.1"
                     /translation="METKELLRNVRRIEIKTRGLVNNVFSGEYHSVFKGRGMSFSEVR
                     EYQFGDEIRFIDWNVSARLD"
BASE COUNT          761 a          625 c          793 g          839 t
ORIGIN      
        1 gcgcgcgtat attcatactg gcattaccgc ggctgatttg caggcgttcg cacatatcgt
       61 ccaacgtgag cggtttagcg ctggcaaaaa gtaatgcgta aagctgaccc attactttat
      121 tgatacccca aaaggtgcta atttcacccc acgttgccgt aaacagttct tgcgatttat
      181 gcagcgcttt atgcatgttt tgttagtttt aaactttcaa aaataattga aacttcgcca
      241 aagtacactt ccgaacgccc attgtcaagc gcaatggtaa ttgcagggat tgaattttta
      301 tacccttgaa cagagcaggg cgattgagta atattgaaat acgattttaa taaaaagcat
      361 gttatgccat ctccaaagac acacgaatat tatatccggc agacgttgaa actggcgcgt
      421 aaaggcgtag gtaaaaccaa aacgaacccg ctcgtcggtg ccattttggt gcgccatacc
      481 ccaaacggtg atcaaattat cggacgaggt tatcatacgg ggtacggaaa gccgcacgcc
      541 gaagtcgaag ccgtacgtga tgcgcagcgt cgcggggtga ccgattttaa aaataccata
      601 ttgtatgtta acttggagcc ctgctgtcat acgggaaaaa caccgccgtg cacgtctttg
      661 atcttacaag aaaaaattcc acgtgtcgta tttgggatca aagatccgtt tccggctgtg
      721 gcaggaaaag gcgccgcgct tctaaagaaa aacggagttc gtgtggagtg gggcatagcc
      781 gagagtgaat gccgtattga aaatcggttt tttttgaagc acgtggaaac aggtttgccg
      841 tggatcacta taaaaacggc gcagactttg gacggaaaaa cggccgatgg tttcggcgct
      901 tccaagtgga taaccaatga aaaatcccga cgcgaagtgc accgcttacg tgcagggcac
      961 gatgcgattt tggtcggtgc acagaccgtg atggcggacg atccgcaact cacggtacgc
     1021 ggcgttcacg ggtcttcgcc gcgtcgcgtc atcgtggatg gccggctgcg ctcacctttg
     1081 acagcgcggg tgttcaccga tgccggcgct tcggaaacca ttgtcattac gactttagga
     1141 gcgaacaaac acaaaaaaag gcagttggca tcgttgggcg taacggtcgt tgagatgtct
     1201 tccgtgcgaa atcggatagc catgcgttcc atatttagaa cgcttctgcg gaaatttcaa
     1261 gttgcgtcgg tattcgtcga aggcggagcg gatatccatg gacagatttt gaatgcggga
     1321 ttgtgcgatg aaatacatgt ttttacggcc gctaaaattt tgggacatgg gaaatcatct
     1381 tttgacggcc tacgcgtcaa acgtattaat aatgctgttg ttttagccga gccccgggtt
     1441 cggatactgg actcggatat ttggatcagc gcacggtggc agcgatgatg acgatgggaa
     1501 atataatcac ggcgagtttg tggggtttag gtattggttt tatcaattcc attccattcg
     1561 gcccgatcaa tatgactatc gtggatcaaa gttttcgccg cggctttcag ccggctatga
     1621 tgatcggctt gggcgcactg gttgtggata tcggttactg tatcatcggt gttttcggta
     1681 tgtccgcttt acaacaatca ttgatcgata tgttacagcc agccggcatg ccggtgctcg
     1741 tttttttggg atatcgtctg atcatcacac gtcataacgg caacgtatat cacgaacgta
     1801 ccgagttttc agctggacta ttgatgaaaa attttattaa cggatgcata atgtatttat
     1861 gcaatccgtt ggctattggc ttttggattg cggcagcggg ttttatgtac gcgtatcaat
     1921 ttttacaatc gtcccttcag tctcaattgg cgtttattgc cggtatggcc atcggcacgg
     1981 ccgcgtggtt ttttcttctc gccgagtggg ttgccgtacg tcgtcagacg atgaaggaaa
     2041 caacgattcg acgcatagct gtaattaccg gttgggtact gatcgtgtct ggttttatat
     2101 tgggttggcg gtacgtgtcg tataaaggat ggatcgcgtg ataaaaatcg aagtgatgtt
     2161 ttttgatctg gatgggacat tagtggattc actcgatgat atcggcgcgt ctgtcaatca
     2221 tacattaaca acgttgggat atagcgcatt acctaaagaa aaactgcgtc gctttgtcgg
     2281 tgacggcatg caaaccttac ttgcgcgtgc cttatctgaa tttgagaaac cggataacga
     2341 tcagttgatg acggccgtat ctatttatcg gacgcaccac gacgaacatt gtcttgattt
     2401 tgttcgcacg tacgacggag ttcttgatgt attgactcat tttaatcact gtcgtaagtt
     2461 tgtgattagt aataaagcgg aagtgtttac tcggaaaatt attcacggcc ttggattgtc
     2521 ccggtatttt gaaggggtat acggcggcgg ggattcgttt gcaaataaga aaccgcatcc
     2581 gcaggctatt caggccatgt tagaaaaaac cggcgtacat cccgatcggg caatgatcat
     2641 cggcgatggt ccgcaagata tcgaagcggg gcgtcgcgca ggggtttata catgcgccgt
     2701 tcgttatggg tttcatgctg ttgcaccgga aggtgcgaat ttttatattg atcgaatgat
     2761 tcagctcaaa gaatttgtag catgatacgg acgtattacg catttaatca tagttaagcg
     2821 gattttatgg aaaccaaaga attacttcgc aatgtgcggc gaatcgaaat taaaacacgc
     2881 ggattggtca ataatgtttt tagcggtgaa taccattcgg ttttcaaagg gcgcggaatg
     2941 tctttttcag aagtgcgcga atatcagttc ggcgatgaaa tccgttttat tgattggaat
     3001 gtctcggcgc ggttggat
//
LOCUS       DAOQNQ010000356         3014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_17118_length_3014_cov_5.505914, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000356 DAOQNQ010000000
VERSION     DAOQNQ010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17118_length_3014_cov_5.505914"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PK754_14780"
     CDS             complement(<1..445)
                     /locus_tag="PK754_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNB10815.1"
                     /translation="MCHDVDFVVIGSGFGGSVSALRLAEKGYNVLVIEKGKHLRASDF
                     PKTNWNVRRWMWIPLLRCFGLFKLTFFRHVTVLSGVGVGGGSLVYANTLPVPKENFFT
                     SPSWSHLADWKEELKPFYPLALRMLGATRNTRMDAGDFALQRMAKK"
     gene            830..958
                     /locus_tag="PK754_14785"
     CDS             830..958
                     /locus_tag="PK754_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10816.1"
                     /translation="MSKERKQLTRDRKIETVRLSYQRDSVVEFAGELGLRPALIYR"
     gene            complement(1609..1746)
                     /locus_tag="PK754_14790"
     CDS             complement(1609..1746)
                     /locus_tag="PK754_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10817.1"
                     /translation="MKEYIRLSEREHEEISGMPAQNFFFRQMAMRLNRHREGCCHNRY
                     S"
     gene            1771..2274
                     /locus_tag="PK754_14795"
     CDS             1771..2274
                     /locus_tag="PK754_14795"
                     /inference="COORDINATES: protein motif:HMM:NF023295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HNB10818.1"
                     /translation="MTVIILYRANDVFDAAGLKKPSIEILDERFLQELKDLPQKNLAV
                     KLLKKLLKDEIKKRTKINLVENKKFSEMLEDAVKRYHNGMIDTVEFLEKVLIPFAEHI
                     KAADKRGEALGLDYREYAFYTALEVNNSAVSILGDATLKSIAVELLKSVRGSTTIDWT
                     IKENSAS"
     gene            2300..2407
                     /locus_tag="PK754_14800"
     CDS             2300..2407
                     /locus_tag="PK754_14800"
                     /inference="COORDINATES: protein motif:HMM:NF023295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10819.1"
                     /translation="MRKFGYPPDLQEKAVETVVLQAKLLADEIALSNKF"
     gene            2575..>3014
                     /locus_tag="PK754_14805"
     CDS             2575..>3014
                     /locus_tag="PK754_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNB10820.1"
                     /translation="MYYVGLATDLRTRVKQHLKDRHSDKWDTFSLYLIKNEHFLKELE
                     SLILHITHPKGNVQRGRFAKSLDLLPVLDGMMTARDRRKRESILDTAARRKRLPASGT
                     SVRSRNLENDPHPALKPGMKLQAVYKGVLYGARVDQSGRIRYGG"
BASE COUNT          907 a          604 c          731 g          772 t
ORIGIN      
        1 gtttcttagc catccgttga agagcaaagt cgcccgcgtc catgcgtgta ttacgtgttg
       61 cgccgagcat acgaagcgcc agcggataaa acggttttaa ttcttctttc caatcggcta
      121 aatgagacca tgaaggcgaa gtgaaaaagt tttctttggg taccggaagg gtattggcat
      181 aaaccagcga cccgccgcct acaccgacac cggagagcac ggtgacgtga cgaaaaaaag
      241 tcagtttgaa taatccaaaa catcgcagta gcggaatcca catccaccgt cgcacattcc
      301 agttggtctt aggaaagtcg cttgcgcgaa ggtgttttcc cttttcgatt acaagtacat
      361 tatatccttt ttcggccagt cgtaacgccg acaccgagcc gccaaatccg cttccgataa
      421 cgacaaaatc tacatcgtgg cacatatcat tgtatccggt gtgattcctt gcaagtcata
      481 gtatgactaa gttattcaac gtgataatga atgtaaagaa tgcaatgcaa tagtgaaaga
      541 aacattctga attctgcgat agaaaaagat gaatttgatt aacataagtc atagttggtt
      601 ttgcggttta tggagtgaga gttaagtgat ggtagaacga cattgagaca cgtcgtcatg
      661 ggaatctatg ggtggttctc aagcagatgg gaaagaacaa aaaaagtgcg ttagataaga
      721 ttgggcggat gaagttgttc gagatgttga accgcagtta gtttggaatt gttacatgtg
      781 aatggacagt tgattaaggt atattgaaca aaccaaatga gctctaggca tgagcaagga
      841 aagaaagcaa cttacgcgcg atagaaagat tgaaaccgtc cgattgagtt atcagcgtga
      901 cagtgtagtt gagtttgccg gtgagttagg actgcgccca gcgttgatat atcgctagcg
      961 gagtgaattt gcggctactg acgagaaaag ttttccaggt aatgggaatg gccagtcaag
     1021 tgaagagtag gtcacacgtc ttaagcgtga actggcaggt attcgcatgg agtgcggcat
     1081 actaaaaaaa actgtgccca tcttcagtga gtatcaatga taaaatatca gatcattgat
     1141 acccattgcg atcaatattt tgtggagaag atgtgtcaag tattggaagt atcgcgcaac
     1201 agttattacc accagcttac acataaaaaa aggccgcctt gggccaggca aacgagagta
     1261 ttttgatgga tttatacgcc aaatatggct tgtgagcaag gaaagatatg aagcacgaga
     1321 gattcgcatg gaactcataa aacaaggcct ccgagccggt cgtgctcggg ttcaactccg
     1381 tatgaaagcc atgaatatac gatgtaccta ccgaccgaaa ttccgagtaa cgaccgatag
     1441 caaacatcat ctttctattg cggaaaacct acccgcgcag agatttaatt cgagcagctt
     1501 tcccaagtgc gaatcagtga tatgacctac gtgcgtcatc tttccacgtg gatttatttg
     1561 accattgtta taggtttagt ggatcggctg atcatcggat gatatttatc agctgtatct
     1621 attgtgacaa cagccttcac gatggcgatt caggcgcata gccatttggc gaaagaagaa
     1681 gttttgagcc ggcatgccgc tgatttcttc atgttcgcgt tcacttaatc ggatgtattc
     1741 tttcataaga atctaagcaa gtaaagcctg atgacagtga ttatattata tagggctaat
     1801 gatgtttttg atgcagccgg tttgaaaaag ccaagcattg aaattttgga cgaacgcttt
     1861 ttgcaagaac taaaagactt gccacaaaag aatttggcag ttaaattatt aaagaaacta
     1921 ctcaaagacg aaataaagaa gcggacaaaa atcaatttgg ttgagaacaa gaagttttct
     1981 gaaatgttgg aagatgctgt caagcgctac cacaacggca tgattgacac ggttgagttt
     2041 ttagaaaaag tattaatacc gtttgccgaa catataaaag cggccgataa gcgtggtgaa
     2101 gcattaggtt tggattacag agaatatgcg ttttacaccg ccttggaagt aaacaacagc
     2161 gccgtttcta ttctaggtga tgcgacatta aaaagtattg ctgttgagct gctcaaatcc
     2221 gttcgtggca gtaccacgat tgattggacc atcaaagaaa acagtgcaag ctgatttgcg
     2281 aagaaatatc aggcgtatat tgcgtaaatt cggatatccg cccgatctgc aagaaaaagc
     2341 ggtagaaacg gtagtattac aagccaaatt gctggctgat gaaatcgcgt tatccaataa
     2401 attttaattt tcatattggc ttctcgtata cctatgccca cagttaaagg acaagaattg
     2461 attacaggca atctggagcg gatttccagt aaagtatttg atcactacca tgacgagatc
     2521 accgatctga tcggcaaaag tcacggggtg taagcgttat acaaagagga tcgtttgtac
     2581 tacgtcggtc tggcgaccga tctgcgcaca cgggtcaagc aacatctgaa agatcgccat
     2641 tccgataagt gggatacctt tagtttgtac ctcatcaaaa atgaacactt tctcaaagaa
     2701 ctggagtcgc tcatcctgca tatcacccat cccaaaggca atgtccagcg cggacggttt
     2761 gccaagtcgc ttgatctgtt gccggttttg gacggtatga tgacggcgcg tgaccgacgg
     2821 aagcgcgaat ccatattaga taccgccgcc cggcgaaagc gcctgcccgc gtccggtaca
     2881 tccgtacgtt ctcgcaattt ggaaaatgac ccgcatcctg cgctcaagcc gggtatgaaa
     2941 ctgcaggccg tttataaagg tgtgctgtat ggcgcacgcg tggatcaaag cggtcgaata
     3001 cgttatggcg gaaa
//
LOCUS       DAOQNQ010000357         3013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_17126_length_3013_cov_4.392157, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000357 DAOQNQ010000000
VERSION     DAOQNQ010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17126_length_3013_cov_4.392157"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="PK754_14810"
     CDS             complement(<1..139)
                     /locus_tag="PK754_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB10821.1"
                     /translation="MPKVICVANPKGGVGKTTTSINLSASLAKFGKRALLIDLDPSGA
                     AS"
     gene            complement(175..1722)
                     /locus_tag="PK754_14815"
     CDS             complement(175..1722)
                     /locus_tag="PK754_14815"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNB10822.1"
                     /translation="MSDKRVEELHRLKAEALLGGGQKRIDAQHKKGKLTARERIAVLL
                     DEGSFEELDMLARHRSHDFGLDKERPLGDGVVTGYGTIDGRLVYVFSQDFTVFGGSLS
                     EVHAEKIIKIMNLAMKVGAPVIGLNDSGGARIQEGVVSLGGYADIFLLNTLASGVVPQ
                     ISAIMGPCAGGAVYSPAITDFILMVKNTSYMFVTGPNVVKTVTHEDVSSEDLGGAMTH
                     ASKSGVAHVAAENELDCLMKIRKLYSYMPSNNMEDPPVIPCDDPADRCEEALKTIIPD
                     NPNKPYDIKDVIKLIVDNGDFFEIHEEYAGNIVVGFARLGGRSVGIVANQPAVLAGVL
                     DIDSSIKGARFVRFCDCFNIPLVVFEDVPGFLPGTDQEWRGIIKHGAKLLYAFCEATV
                     PKVTVITRKAYGGAYDVMNSKHVRGDMNFAWPSAEIAVMGAKGAVEIIFKKEIDASAN
                     PKETEEKLTEEYKEKFANPYSAAERGYVDDVIEPQLTRPKLIRALAMLENKVDTNPKK
                     KHGNIPL"
     gene            1824..>3013
                     /locus_tag="PK754_14820"
     CDS             1824..>3013
                     /locus_tag="PK754_14820"
                     /inference="COORDINATES: protein motif:HMM:NF015655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10823.1"
                     /translation="MLESFSHDLSFFHNTAQAETNLKSITNQTQPHDIRFLKRLLDEL
                     RPYPNCDDIFNLFERLSRAVIDRIAFFKMLSDTPPTIARLVRLFASSPFIAELMIRDF
                     QYAYFLVRPEYDCRPFDKLEVQQHILRLFHNQTPSLNKVMDALRKIKRREMLKLGIRD
                     YILQDPLEKTVASVSDLAECVLQGALDAQRRDMAARWGNPETPFCVIALGKFGGGELN
                     YSSDIDVMFVYEEEDEYVANEHRLTHHEYFNRLASGLIAHIAKNTSEGQLYRVDARLR
                     PDGDAGPLARCVSSFLYYYEARGQTWERQMLIKARPVAGDLEFGARFIKRLEPFVYPK
                     TFFQSPLHEVAAMKWRIEEQKSSDHLNIKICPGGIRDIEFIVQALQLIHGGQMTEIRT
                     GNTL"
BASE COUNT          760 a          773 c          662 g          818 t
ORIGIN      
        1 cgctcgccgc gccgctcgga tcaaggtcaa taagcaacgc acgtttgcca aacttggcca
       61 atgacgccga caaattgata gaagtcgttg ttttcccaac gccgcctttg gggttggcaa
      121 cacaaattac tttgggcatt tttttaatta cctttaacgt tccgtagcgt gattttacaa
      181 aggtatattg ccgtgtttct ttttcggatt ggtatctact ttattttcca gcatagccaa
      241 agcgcgaatc aatttgggac gcgtcagttg cggctcaatg acatcgtcca catatccgcg
      301 ttcggcggca ctataagggt tggcaaattt ttctttgtat tcttccgtca gtttctcttc
      361 ggtctctttc gggttggccg aggcgtctat ttctttttta aagatgatct ccaccgctcc
      421 tttagcgccc atcacggcga tttcggccga cggccaggca aagttcatat cgccacgcac
      481 gtgtttggaa ttcatcacgt cgtacgcacc gccataagct ttgcgcgtga tgaccgtgac
      541 tttgggcacc gtcgcttcac aaaatgcata gagcaattta gcgccatgtt tgataatgcc
      601 gcgccattcc tgatccgtac ccggcaagaa tccggggaca tcttcaaaaa caacaagcgg
      661 tatattgaaa caatcacaaa aacgaacaaa ccgtgcgcct ttgatcgaag agtcaatgtc
      721 cagcacacct gcgagtaccg ccggttggtt ggcgacgata cctacggaac gccctccgag
      781 tcgtgcaaat ccgacaacga tattgccggc gtattcttcg tgtatttcaa aaaaatcacc
      841 gttatccacg atgagtttga ttacgtcttt gatatcgtac ggtttattag ggttgtccgg
      901 aatgatggtt ttgagcgcct cttcacaacg atcggccgga tcatcgcagg gtatcaccgg
      961 cggatcttcc atattattgg atggcatata gctgtacaat tttctgattt tcatcagaca
     1021 gtccaattcg ttttccgccg caacgtgtgc aacaccgctt ttgcttgcat gggtcatagc
     1081 gccgccgaga tcttcagaac tcacatcttc atgcgtaacc gttttgacca cattgggtcc
     1141 ggtgacaaac atatagctgg tatttttgac catcagaata aaatcggtaa tcgccggaga
     1201 atataccgcg ccaccggcac acggtcccat aatcgccgaa atttgaggca cgactcctga
     1261 ggccagtgta ttcaaaagaa aaatatccgc gtaaccgccg agacttacga cgccctcctg
     1321 aattcgcgca ccgcccgaat cgttaagacc gatcaccggc gctccgactt tcatggcgag
     1381 attcataatt tttatgattt tttccgcatg cacttccgac aaactgccgc cgaataccgt
     1441 gaaatcctga ctgaaaacat acaccaaacg gccgtcaatg gtaccatacc ccgtgactac
     1501 gccgtcaccg agcggtcttt ccttatccaa accaaagtcg tgcgaacgat gacgtgcgag
     1561 catatccaat tcttcaaaac taccttcatc taaaagaaca gcgatacgtt cccgtgccgt
     1621 aagtttacct tttttatgtt gcgcatcaat gcgcttctgt ccaccaccca gcaaggcctc
     1681 tgctttaagg cgatggagtt cttctacccg tttatcagac atatagaggc tccggagtat
     1741 ttatgattgg aattgttatt tttagaagta aattcataga cattaaaact tacggaatcg
     1801 tcttttcttt ttcaaactaa aacatgcttg aatctttttc acatgacctg agtttttttc
     1861 ataatacagc acaggccgaa accaatctga aaagcattac aaaccaaacc caaccgcatg
     1921 atatccgctt tctcaaacgg ctattggacg aattgcgccc atatccgaat tgcgacgata
     1981 tattcaattt atttgaacgc cttagccgtg cggtgattga tcgcatcgct ttttttaaaa
     2041 tgctcagtga tacgccgcct acaattgcac gtctggtgcg gctctttgca tccagcccat
     2101 ttatcgcgga actaatgatc cgtgatttcc aatacgctta ttttctcgtt cgccccgagt
     2161 acgattgccg tccttttgac aaacttgagg tccagcagca tattctccgc ctttttcata
     2221 atcaaacgcc gtcgctcaac aaagtcatgg atgcattgcg gaaaattaaa cgccgtgaaa
     2281 tgctgaaact gggtattcgg gattatatac tacaggatcc gctcgaaaaa accgtcgcat
     2341 ccgtgagcga cttagccgaa tgtgtactgc aaggcgcttt ggatgcccag cgtcgggata
     2401 tggcggcacg ttggggaaat ccggagacac cgttttgtgt aatcgctctt ggaaaatttg
     2461 gcgggggcga gctgaattac agttccgata ttgatgtgat gtttgtgtat gaagaagaag
     2521 acgaatacgt cgctaacgag catcgcttga cccatcatga atattttaat cgcttagcgt
     2581 ccggcctgat cgcacacatt gctaaaaata catccgaggg tcagctttac cgtgtggatg
     2641 cgcgactccg tcctgacggc gatgccggtc cgcttgcgcg gtgtgtttcc agttttttgt
     2701 attattacga agcgcgcggt caaacgtggg agcggcaaat gcttatcaag gccagacccg
     2761 tggccggcga tttggaattc ggcgctcgtt ttatcaaacg gttggaacct tttgtgtatc
     2821 ctaaaacttt tttccaatcg cccttgcacg aagtcgccgc catgaagtgg cgcatcgaag
     2881 aacaaaaatc ctccgatcac cttaacatca aaatatgccc cggcggaatc cgcgatatcg
     2941 aatttatcgt acaagcgctt cagcttatcc acggcggaca aatgacggaa attcgaaccg
     3001 gaaacacact caa
//
LOCUS       DAOQNQ010000358         2985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_17421_length_2985_cov_4.916382, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000358 DAOQNQ010000000
VERSION     DAOQNQ010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17421_length_2985_cov_4.916382"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PK754_14825"
     CDS             <1..341
                     /locus_tag="PK754_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10824.1"
                     /translation="AQDKSYNTDQLKSAKRISEDQWKGMLDASSQRSNGFVTAAKGTT
                     DYMATQLGYTVLKYNQTIELKAEVIAVLEGVYSAGQSNAGTAGTRNPKTFKLGPGKYE
                     VKEGIIKRVP"
     gene            complement(526..831)
                     /locus_tag="PK754_14830"
     CDS             complement(526..831)
                     /locus_tag="PK754_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10825.1"
                     /translation="MIAIEKFSKDIKYVFNSTRTHVLCIKETRPEHPPKIHVMSFLLI
                     EAITGHIILEETTTNAQAEWISESEVRVTFITGMPKENSHSAVGYIYNVISKVKTDL"
     gene            1222..2334
                     /gene="dprA"
                     /locus_tag="PK754_14835"
     CDS             1222..2334
                     /gene="dprA"
                     /locus_tag="PK754_14835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNB10826.1"
                     /translation="MSLTHRSHKDKNFDLLRLIGVRGMGHARLRNLIRHFGSPASVLS
                     ASPKALAAVQGMDAPTAERIKQDHEVSIAFAHAQMRSMQENATELLIFSESGFPELLT
                     QTETAPSFLFVRGAIMPEDAHAIAIVGTRQPTAYGRTMAEKLAQELAQRGITVVSGLA
                     RGIDTVAHAAALRSGGRTIAVLGSGVDHIYPSENYRLAMEIAHRGAILADYPMGTGPD
                     ATHFPGRNRIISGLSLGTVVIEAGEKSGALITADYALDQNREVFAVPGLATNPQAKGS
                     NRLIRSGAKLVETVDDILQELEGRLAQTKRIPTPAIQLSLEEQSVYEKLSPEPLHIDA
                     IAEAARMNAHEALSHLLQLEIRGAVRQLSGKHFVKS"
     gene            2355..2714
                     /locus_tag="PK754_14840"
     CDS             2355..2714
                     /locus_tag="PK754_14840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB10827.1"
                     /translation="MPKKVLIVDDENTVRSLLKIVLGKAGFEMDFAEDGLQAMEKVKS
                     FDPDVIILDTNMPHKSGIEVCSEVKAVNPARPKIIFHTGSLEEYKAKAEAAGADATFA
                     KTGMGGMGKLVELIQTM"
     gene            complement(2791..>2985)
                     /locus_tag="PK754_14845"
     CDS             complement(2791..>2985)
                     /locus_tag="PK754_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10828.1"
                     /translation="YIKKLFKKKDKEFFDLIAALNASKNWKDANNIIDDEFYRLEINP
                     YQSEAIAFTDAVYSRYFPKS"
BASE COUNT          859 a          603 c          697 g          826 t
ORIGIN      
        1 ccgcacagga taaatcctac aatacagatc aattgaagtc ggctaaacgc atttcggaag
       61 atcagtggaa aggtatgttg gatgcgtcat cgcagcggag caatggattt gtaacggcgg
      121 ctaaaggtac gacggactat atggcaacgc aattaggata cacggtttta aaatacaatc
      181 aaacgattga acttaaagca gaagtgatcg ctgttttaga aggcgtttat tctgccggac
      241 agtccaatgc cggtacagcc ggtacacgta atcctaagac ctttaagctt ggcccgggaa
      301 aatacgaagt caaagaaggt attatcaaaa gagttccgta gttttgtaat gcgataagtt
      361 tttgattcgt cttttaattt caacttccac ataaaaaaac ctcccgaaaa aatcgggagg
      421 tttttcgttt atagcatccg atactgatta agtagaatta ataatgaatt ttagaatatt
      481 aaaattcttt taccatcatt agataatcaa ttctaaaagg atatcttaca agtcagtttt
      541 tacttttgaa attacattat atatgtaacc gacggcgcta tgactattct ctttgggcat
      601 tcctgtaata aatgttactc gtacttcaga ttcggaaatc cactcagctt gagcattagt
      661 tgtggtttct tccagtatga tatgacctgt aatagcttcg attaataaaa aactcattac
      721 gtggattttt ggtgggtgct caggcctggt ttctttgata cataaaacat gcgtgcgcgt
      781 actattgaat acgtatttaa tatcctttga aaatttttcg atagcaatca atttatattt
      841 ttccgcatct tgctgtgcct caacctgtgt acgcgatgca ctgcatgccg caatcagaga
      901 aaaaaagcgt agattttacg atcataaaat cctcataata atttaacaaa aagatgcgaa
      961 cgatttcagc cgccccatat acggcatgta ctttataaga acttgtactt gaaaggtaaa
     1021 ataatttatc taaaaggatt ataaagtcaa gcggaaatgg ttaagtagct gttttgaata
     1081 gttataaagt atggtgcttt ttcacgtcgc tcggggattc atttttaaga aaaacaaatc
     1141 tgcatatctt gcatttatgc ggagtgcata ttacattacg cggcttttcg attggggaac
     1201 ttcaaacgaa ttgatttatc tatgagcttg acgcatcgtt cgcataagga caaaaacttt
     1261 gatttattac gcctgatcgg tgtgcgcggt atggggcatg cccgattgcg caatcttatt
     1321 cgtcactttg ggtcgcccgc atccgttctc agcgcttcgc ccaaagcatt agctgccgtg
     1381 caaggcatgg atgcgccgac cgccgagcgc atcaagcagg accatgaagt aagtattgct
     1441 tttgctcatg cgcagatgcg atccatgcag gaaaacgcga cggaattatt gattttttcc
     1501 gaatcaggat ttccggaatt actcacacaa actgaaaccg caccctcgtt tttgtttgtg
     1561 cgcggggcga taatgccgga ggatgcccat gcgatagcga ttgtcggaac acgtcagccg
     1621 acggcgtacg ggcgtacgat ggcagaaaaa cttgcgcaag agttagccca acgcggtatt
     1681 accgtcgtca gcgggttggc gcggggtatt gatacggtag cgcatgcagc ggcattacgc
     1741 agcggaggac gtacgatcgc cgtgctgggt tccggcgtgg atcatatcta tcccagtgaa
     1801 aattacagat tggctatgga aatagcgcac cgcggtgcga tcctggcgga ctatccgatg
     1861 gggacaggcc cggatgcaac gcattttccc ggacgaaacc gaatcatcag cggactttcg
     1921 ctcggtacag ttgtcattga agccggcgaa aaaagcggcg ctctgattac ggcggattat
     1981 gcgctggatc agaaccgcga ggtgtttgcc gtgcccggct tggctaccaa tccgcaagcc
     2041 aaaggcagta atcgcctgat ccgtagcggt gctaaactcg tcgaaacggt agatgatatt
     2101 ttgcaggaat tagaaggtcg tttagcgcaa acaaaacgta tacctacacc ggcgatccaa
     2161 ctcagcttgg aagaacaatc ggtgtatgaa aaattatcac cggaaccgct tcacattgat
     2221 gctatcgcag aagctgcccg tatgaatgca catgaagcgc tgtcccatct tttacaatta
     2281 gaaatccgcg gtgccgtgcg tcagctaagc ggtaaacatt ttgtgaaatc ttaaatctta
     2341 cgaaggaata caccgtgccc aaaaaagttt tgatcgtgga tgacgaaaat accgtacgca
     2401 gccttttgaa gatcgtatta ggcaaagccg gttttgaaat ggattttgcg gaagacggat
     2461 tgcaagcgat ggaaaaagtc aaatcatttg acccggacgt gattatcctg gataccaata
     2521 tgcctcataa aagcgggatc gaggtgtgca gtgaagtaaa ggccgtcaac cccgcgcggc
     2581 ccaagattat ttttcatacc ggatcgctcg aagaatataa agctaaagca gaggcggcag
     2641 gtgccgatgc gacgtttgcg aaaacgggta tgggtggtat gggcaaatta gttgagttga
     2701 tacaaacgat gtaacatgtt tttaaaatat actgagctta aattaaaaaa cccggcgtta
     2761 ccgggttttt ttaatttaat ttttttgatt ttagctctta ggaaaatacc gcgaatacac
     2821 ggcatcagta aaggcaatag cttcactttg gtacggattg atttcgagcc gatagaattc
     2881 gtcgtcaatg atgttatttg catctttcca atttttggag gcgttgagcg ccgcaatgag
     2941 atcaaaaaat tctttgtctt ttttcttaaa gagttttttg atata
//
LOCUS       DAOQNQ010000359         2984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_17439_length_2984_cov_4.730966, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000359 DAOQNQ010000000
VERSION     DAOQNQ010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17439_length_2984_cov_4.730966"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PK754_14850"
     CDS             <1..772
                     /locus_tag="PK754_14850"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNB10829.1"
                     /translation="IDLDPSGAASVGVGFTRENATFGVYDLYKHPESLDEAIVRKAIY
                     ASGTERLDVMPSNVWTSEEENELLKLSAKLTRLRIMINFVRNDYDYFIIDCPPFLSNL
                     TLGAFTASDSTIVPVQCGFFALHALERLMKFYRKVRESINPYLKIEGILITMYEKGTN
                     VSIKTVRDARAVFEDLVFGVIIPKNVKIGDAAFQKKPVVTYDSTSAGAKAYIQLAEVI
                     LHRDDPQWDIQAHVQTPSEPRNESQTIPDVVSSSGENA"
     gene            874..1473
                     /locus_tag="PK754_14855"
     CDS             874..1473
                     /locus_tag="PK754_14855"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNB10830.1"
                     /translation="MTDTFLPRVLGKIILASASPRRAQILKQVGFDFDVMPSAIEEVS
                     EHHEPVEYAREIAQKKALDIAQKYPERIVIAADTIVCLDGHILGKPSDEEDAWRMLRF
                     LSGRNHQVYTAFAISHRSRNVNMVEHELTQVHFRRLRDEEIAMYIATGAPFDKAGAYG
                     IQDASAIFVDRIDGDFYNVVGLPVTRLYQVLTDTYLEKR"
     gene            1470..2252
                     /locus_tag="PK754_14860"
     CDS             1470..2252
                     /locus_tag="PK754_14860"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNB10831.1"
                     /translation="MSNAYYKELSGWLRQARADQHITAVAVALRTRMPLETIEKIEAG
                     DLDFATPVFIQGYLKNYADAIGLDSAAVVREYKVIRAKIAYVTSDRGQVVARATAQHS
                     GNPDELSSEIIPFDPESHAFAPHSELDQAVRKKISTEKWTNIIMMSSAACMVILSLYW
                     VVSDDHSQDEETAANLAKYERKAETFKQAKIEPRKRLSFYFSEPILTDEEINRKLEVR
                     EREQQDSMRLAIYQKNQKWSHGQKVRAITVQEAALDYAQSMK"
     gene            2291..>2984
                     /locus_tag="PK754_14865"
     CDS             2291..>2984
                     /locus_tag="PK754_14865"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNB10832.1"
                     /translation="MLKKLNDMKLRAKLMAGFGVFSAIFITTVTIVFTVKLHTAAVSH
                     QSLILRSFAELTAKNISAGLDFGDKGSVVSAFDALRTHAEFVMVHDVSGENFATFYRD
                     PKIENRIDPVVNEQIGRWREAKKSDWTFDALDMNCIVVPVFNSAGNEIGNLSLGMSTK
                     SINDELQSNIFFSFILLIVTVGVGIGVVYWISGLLVRPLMNIVERFKDIAEGEGDLTR
                     RIHIDAKDEVGEL"
BASE COUNT          837 a          609 c          759 g          779 t
ORIGIN      
        1 tattgacctt gatccgagcg gcgcggcgag cgtcggcgtc ggttttacgc gtgaaaacgc
       61 aacatttggc gtgtacgatc tgtataaaca tcccgaaagt ttggatgaag cgattgtaag
      121 aaaagcgata tacgccagcg gtaccgaacg tctggatgtg atgccgtcca atgtatggac
      181 gagcgaagag gagaatgagc ttttgaaact ctctgcaaaa ctcacgcggc ttcgcattat
      241 gattaatttt gtccgtaatg attatgatta tttcatcata gattgccctc cttttctcag
      301 taatctgacg ttgggcgcat ttacggcatc ggattcgacc atcgtgccgg ttcaatgcgg
      361 tttttttgcg ttgcatgctt tggaacgact gatgaagttt taccgtaaag tgcgcgaatc
      421 cattaatccg tatctcaaga ttgaaggtat tttgatcacg atgtatgaaa aaggcacaaa
      481 cgtcagcata aaaaccgtgc gcgatgcacg tgccgttttt gaagatttgg tttttggagt
      541 gattatccct aagaacgtaa aaatagggga cgctgcattt cagaaaaaac cggttgtcac
      601 gtatgattct acatccgccg gtgcgaaagc gtatatccaa cttgcggagg ttattttaca
      661 cagggatgac ccccaatggg atattcaagc gcatgtacaa actccgagcg agccccgtaa
      721 cgaatcccag acgatcccgg acgtagtatc ttccagtggg gaaaatgctt gaccaaggac
      781 ggcaaaaaag gcttgtaact tcctgaaaaa acaaatacat tgcacacgct gtacacgtcg
      841 aagattcata cccaaattct tattcggttt attgtgacag acacattttt gccgcgtgtt
      901 ttaggaaaaa tcattttagc atccgcttcg ccgcgacggg cacagatatt aaaacaagtc
      961 ggttttgatt ttgatgtgat gccgagcgcc attgaagaag tttctgaaca tcacgaaccg
     1021 gtcgagtatg cgcgcgaaat tgctcaaaaa aaggccttgg atattgctca aaaatatccc
     1081 gagcgaattg ttatcgcggc ggacacgatc gtgtgtttgg acgggcatat tttaggaaaa
     1141 ccgtccgatg aggaagacgc atggcgtatg ctgcgttttt tgagcgggcg taatcatcag
     1201 gtatatacgg catttgccat atctcaccgg tcacgcaatg tgaatatggt ggaacacgaa
     1261 ttgacacagg tgcactttcg ccggttgcgg gatgaagaaa tcgccatgta cattgccacc
     1321 ggagcgccgt ttgataaagc cggggcttac ggcatacagg atgccagcgc tatttttgta
     1381 gatcgtattg atggcgattt ttacaatgtg gtggggttgc ctgtgacaag gttgtatcag
     1441 gtattgacag acacgtattt ggaaaaacga tgagtaatgc gtattacaaa gaactttccg
     1501 gatggctgcg tcaggcgcgt gcggatcagc atataacggc ggtggcggtt gccttgcgta
     1561 cccggatgcc tttagaaacg atcgaaaaaa tcgaagccgg cgatttggat tttgctacac
     1621 cggtttttat acagggatat cttaaaaatt atgcggacgc gatcggcctt gactcggcgg
     1681 ctgtcgtgcg ggaatataaa gtaattcgtg caaaaatagc gtatgtgacg tcggaccgtg
     1741 ggcaagtagt agcacgtgct acggcgcaac acagcggaaa tccggatgaa ctttcgtctg
     1801 aaattatacc gtttgatccc gaatcccatg cgtttgcgcc gcactcggaa ttggatcagg
     1861 ccgttcgaaa aaaaatttca accgaaaaat ggacgaatat catcatgatg agttctgcgg
     1921 cttgcatggt gattctgagt ctgtattggg tcgtctccga tgatcattcg caagacgaag
     1981 agacggcggc caatttggcc aaatacgagc ggaaagcgga aacatttaaa caagctaaaa
     2041 tcgaaccgcg taaaaggttg tcattttact tttccgaacc tattctaacg gacgaagaaa
     2101 tcaacaggaa attggaagtc cgcgaacgtg aacagcagga cagtatgcgt ttagcgatat
     2161 atcaaaaaaa tcaaaaatgg tcgcacggcc agaaagtacg cgccatcacg gtacaagaag
     2221 ccgccttgga ttatgctcaa tcaatgaaat aaattttaag acgaagatag cgttttcggg
     2281 agatgaaatt atgctaaaga aactcaatga catgaaacta cgagccaagc tcatggcggg
     2341 attcggcgtt ttttcagcca tttttataac taccgtaacg atcgttttta cagtcaaact
     2401 tcacaccgcg gctgttagcc accagtcact gatactgcgc agctttgccg aattgactgc
     2461 caaaaacatc agtgcgggat tggattttgg cgataaaggt tcggtggtca gcgcgtttga
     2521 tgcactcaga acccatgccg aattcgtaat ggtgcacgac gtaagcggtg aaaactttgc
     2581 tactttttat cgcgatccta agatagaaaa tcggattgat cccgtggtca acgaacaaat
     2641 cggtcgctgg cgtgaagcta aaaaaagcga ttggacgttt gatgcgttgg atatgaattg
     2701 tatcgtcgta ccggtattta acagcgccgg taatgaaatc ggaaacctct cattgggtat
     2761 gtcaaccaag tcaatcaatg atgaattgca atccaatatt tttttcagtt tcatacttct
     2821 tatagtgact gtcggagtgg gaataggtgt cgtgtattgg attagcgggc ttttagtaag
     2881 gccgctgatg aatattgtgg agcgatttaa ggacatcgcc gaaggcgaag gtgatttaac
     2941 gcgtcggatt cacattgatg ccaaagatga agttggtgag ttag
//
LOCUS       DAOQNQ010000360         2949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_17845_length_2949_cov_5.211818, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000360 DAOQNQ010000000
VERSION     DAOQNQ010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17845_length_2949_cov_5.211818"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PK754_14870"
     CDS             complement(<1..181)
                     /locus_tag="PK754_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10833.1"
                     /translation="MKRMFVVMMLTAGTLFSQNPAVSIAIEGKNKIQNGFAAWNETMI
                     REGSALCERAAMADPQ"
     gene            complement(211..918)
                     /locus_tag="PK754_14875"
     CDS             complement(211..918)
                     /locus_tag="PK754_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10834.1"
                     /translation="MRYRVWLLVCIILITVSPGSAQQVRKTYGGFGGFIFGASKQDMT
                     GFNKTLKNSGYRTYENQSWMYGGGGFAVLGRFLFGGESHALMARNTVSENALYRVKLR
                     GAVGQFKIGYILLQKGSIRFYPFVGLGDSDFILEIAERIPADFEDILDQPARASRLRV
                     KATTIDAGWGVLHTLFDSGRRSGGGLAIGMQIGYRYTPAKNDWQRFGGDTEGRSIPLW
                     NGPYATLLFGAAGFGVR"
     gene            1337..1621
                     /locus_tag="PK754_14880"
     CDS             1337..1621
                     /locus_tag="PK754_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HNB10835.1"
                     /translation="MALDALGMVETKGLVGAIEAADAMVKAAKVELIGKEKIGGGYVT
                     VMVRGDVGAVKAATDAGAAAAEKVGELVSVHVIPRPHPEVELILPKKGSN"
     gene            1640..1972
                     /locus_tag="PK754_14885"
     CDS             1640..1972
                     /locus_tag="PK754_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013251191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HNB10836.1"
                     /translation="MADERALGMVETLGLVGAIEAADAMIKASNVKLIGKEITDGAMI
                     TIKVVGEVGAVQASVSAGEAAARRVGQVVSVHIIPRPDAMTESIIYDEGLVEAPSGGT
                     GSKKKLNA"
     gene            2250..>2949
                     /locus_tag="PK754_14890"
     CDS             2250..>2949
                     /locus_tag="PK754_14890"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNB10837.1"
                     /translation="MFKLPEWLLRADRRIQELNLFRRIFIMMFPLMIAAIGVFEITIR
                     WFDIHAIEYAHIWIILIPLIFSIIISYLSARFTAKEVAHPIKLFIESAKAIARGNFDH
                     KVNVHTSEEMTQLSRIFNYMTRELKEINQGNIRKTIQEKIKTEAIIRNIADGIIVVGP
                     LNEVLMINEVVEGWFHVQQKAVIGFSLNFFFPELKFLVKQALDTQGETVFQDELTIKP
                     LNSPSEIYLGANASK"
BASE COUNT          813 a          739 c          636 g          761 t
ORIGIN      
        1 tctgcggatc ggccatagcc gcccgttcac acagtgcgct gccctcgcgg atcatcgttt
       61 cgttccatgc ggcaaaccca ttttgaattt tgttttttcc ttcgatggct atagataccg
      121 ccggattttg tgaaaaaagt gttcctgcgg tcaacatcat tacaacgaac atgcgcttca
      181 tacatcctcc tctattgtga taattaaaaa ttaacgtaca ccaaatccag cggcgccaaa
      241 aagcaatgtc gcataaggcc cgttccacaa aggaatactt cgcccttccg tatcgccgcc
      301 aaagcgttgc caatcatttt tagccggtgt gtaacggtat ccgatttgca taccgatagc
      361 caaaccgcct ccggaacgtc gcccgctgtc aaacagcgta tgcaatacgc cccatccggc
      421 atcaatggtc gttgccttga cacggagtct tgaagctctg gccggttgat ccaatatatc
      481 ctcaaaatcc gccggaatac gctcggcgat ctctaatata aaatcagagt cacccaatcc
      541 gacaaacgga taaaagcgta ttgatccttt ttgtaaaagt atgtatccga ttttaaactg
      601 ccccacagcg ccgcgcagtt ttacccgata cagcgcattc tccgataccg tgttgcgggc
      661 catcaaagca tggctttcac cgccaaacaa gaaccgcccc agcacggcaa aacctccgcc
      721 gccatacatc cagctttgat tttcgtaagt gcggtatccg ctattcttga gtgttttatt
      781 aaaaccggtc atatcttgtt tactcgcacc gaaaataaaa ccgccgaatc cgccataggt
      841 tttacgaact tgctgcgctg agccagggct gacggtaatt aggattatac acacgagaag
      901 ccatacacga taccgcataa ctttccttcc atttttaata acaatacgcc caataccgtt
      961 tttttccgac atacttccgc caaactgcag taaatggggg ttgaagtgca caatactgca
     1021 tacgaaaaga ccctttgtgc cgatgcatgc aaggcataca accctatttt caaaaagtgc
     1081 taacgtaggc attgaaaaaa aatttgcttt ccgatgttag attcgttaat atgaagtccc
     1141 cacaagaggt ctccccgacc tctggctacg atagagaccc ggaagcttgt cttccgggta
     1201 tttttttgta atgatgttct ttttatgccg tatgacagac aaaattacat ttgactttca
     1261 tgcgccgttt atataatatc gaatactatt ttgcacactt gatgtcctat ctcaatattt
     1321 tctaggagga atttccatgg ctctggatgc attaggcatg gtagaaacca aaggtttggt
     1381 cggcgcaatc gaagccgctg acgcgatggt caaagccgca aaagtcgaat taatcggcaa
     1441 agaaaaaatc ggcggtggtt acgtgaccgt catggtacgt ggcgatgtcg gcgctgtaaa
     1501 agcagctacc gatgccggcg ctgcagcagc agaaaaagtc ggcgaactgg tttccgtcca
     1561 cgtcattccg cgtccgcatc cggaagtgga acttattctt cccaaaaaag gctccaacta
     1621 atttaacacg gcgaatcgta tggcagatga acgtgcatta ggaatggtgg aaacgctcgg
     1681 attggtcggt gcgatcgaag ccgcggatgc catgatcaaa gcttccaatg tcaaactgat
     1741 cggcaaagaa ataaccgatg gcgccatgat cacgatcaaa gtggtcggcg aagtaggcgc
     1801 tgtgcaggcc tctgtgtccg ccggcgaagc cgctgcacgt cgtgtaggac aagtcgtttc
     1861 cgttcatatc ataccgcgtc cggatgcaat gacggaatcc atcatctatg atgaaggatt
     1921 ggtagaagct ccgagcggcg gcacgggctc aaaaaaaaaa ctgaacgcct gacccttgcc
     1981 caacaagatc tttttaaggg tgttcaaaaa ggcgaatcca tacttaagaa gaaaaagtta
     2041 gttgttcctg aaaccgatta tcatgagttc agcgtttcgg aacttcggca ttatgcacgg
     2101 tctgttccca atttcccgct aaaaggccgc gaaatttcca aagccaaccg ggatatgctg
     2161 atcgaagaac tggataaaat aattcctaaa aaataaacac cctgaacatc cttcagggtg
     2221 ttctttttta cgcttcattt ctacgacgta tgtttaaatt gcccgaatgg cttctgcgtg
     2281 ccgaccggcg aattcaggag cttaatctct ttcgccggat cttcatcatg atgtttccgc
     2341 tcatgatcgc ggcgattggt gtatttgaaa tcaccatacg ctggtttgat atccacgcta
     2401 tcgagtacgc acacatatgg atcatactaa ttccgcttat tttttctatc atcatatcgt
     2461 atttatcggc tcgatttacc gcgaaagaag ttgcccatcc tatcaaactc ttcatcgaaa
     2521 gcgccaaagc catcgcgcgt ggtaattttg atcacaaggt caatgtgcat accagcgaag
     2581 aaatgactca gctttcgcgc atttttaact atatgacccg tgaattgaaa gagatcaatc
     2641 agggcaacat ccgaaaaaca atccaggaaa aaattaagac cgaagccatc attcgcaata
     2701 ttgccgacgg tattatcgtg gtcggcccgc tgaatgaagt tctgatgatc aatgaagtgg
     2761 tcgaaggatg gtttcatgta caacaaaaag ccgtcatcgg tttttcgctc aattttttct
     2821 ttcccgaact caaatttctc gtgaaacagg cgcttgacac acaaggcgaa acggtttttc
     2881 aggacgaact tacgatcaag ccgctcaata gcccttcgga aatctatctc ggcgccaacg
     2941 cttccaaag
//
LOCUS       DAOQNQ010000361         2941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_17935_length_2941_cov_5.094941, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000361 DAOQNQ010000000
VERSION     DAOQNQ010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17935_length_2941_cov_5.094941"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2941)
                     /locus_tag="PK754_14895"
     CDS             complement(<1..>2941)
                     /locus_tag="PK754_14895"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10838.1"
                     /translation="EWVFNHQIDGLITSRSDGHRILGETLKLFIEQDRDSANFGSFDR
                     FYLIAYNHRVFAATSPLTMFFTVPHHATLKTIDIRRTNGTRYFETTLALHERDRDFQM
                     FVHRLIAGHREFSGIYRSVHDYVDKNRPWIKDRELRIAVDAIYANGFSEHDFRSEYTE
                     PKNLKLYLHQVGWRCARDLTNRISERSGFLMDVTKPTDGLIPMVLSKQFTDPGVIYVD
                     ADWNEEVRRQVPVTDPNPLESRTLPGTQIPYPYLLASDLLENSLIKLPYKAHPSFLTL
                     THSDEDESYMLPVKLFYYNYFSPEDLRKQISFTVDGDAVTVRLQIPTRSGKRVELKRR
                     YVPDPIEGTDTGRIVEWKFDVAVFPFYRVKQEAESVERYNVMMVDGQRNRKDFPDLTF
                     FDSRMEPLSESKSKQSPVKKIVRHDKKVFDGGVYYQITGAQFDYIRCELKGPQKAVGW
                     IVPMWRYVEPGTRAYTFAVDFGTTSTHIAYADATGAPKPFDFTKEDMPVVNLYGPEKI
                     SNRDAVTDLYRRYNDRTVLPQSSVYQENDFVPTFIGAAGSPIQFPIRTATSESPGFVR
                     EETFTLGNINMAFNHERHVEFDDEIVHTNIKWKINLDEKTTERIRAFFEEILLLIRHK
                     VVLNQGDPAKTKLIWFHPLSMSAHAIGLLKSDWTQLAQKVLGISEQAVIEMTESQAPY
                     YYHKATTAVVGDSNVLCVDIGGGSTDIVVFEKRQPKFGSSFNFGCNVLWSEGNVLASR
                     VNNKDNGIFREAYQAVFRNAPPNNKEWNQLRDICEQYLANERKRSEDVMQVFFKHEHL
                     TQISERLGKDQKFKIIFLAYYAATVYHALQCMRTKKLQAPHYICFSGNGSRVLGFIDR
                     DPALKNLTAFTRILAEAVYGNGAKEMRLVVSKEPKQATCIGGIVRQKENEQPIEAMIH
                     LGGKKKNDAKGLTYDAVSDGVKKDVQDNIKDFINLFFDLNEQISFHEYFGIEADLAMC
                     RTELQKDI"
BASE COUNT          750 a          737 c          666 g          788 t
ORIGIN      
        1 aatatctttt tgcagttcgg tgcggcacat ggccaaatcg gcttctatgc cgaaatattc
       61 gtgaaaagat atttgctcgt tcaaatcaaa aaatagattt atgaaatctt tgatattatc
      121 ctggacgtct tttttgactc cgtcggatac ggcgtcgtac gtgagacctt tggcatcatt
      181 tttctttttt ccgccaaggt ggatcatagc ttcgatcggc tgctcgtttt ctttttgacg
      241 cacgataccg ccgatgcagg tggcttgttt aggctctttg gaaacgacaa ggcgcatctc
      301 tttggctccg ttgccataga cggcctccgc taaaatacgg gtaaacgccg taagattttt
      361 taacgccggg tcgcgatcaa taaaacctaa aacgcgactg ccattaccgc tgaaacagat
      421 ataatgcggc gcttgcaatt ttttggtgcg catacactgc aacgcgtgat ataccgtggc
      481 ggcatagtaa gcgagaaaaa taatcttaaa tttctgatcc ttacccaaac gttcgctgat
      541 ctgcgtaaga tgttcatgtt taaaaaacac ctgcatcacg tcctccgaac gcttgcgttc
      601 gttggcgaga tactgttcac aaatatcccg cagctgattc cattctttat tgttaggcgg
      661 agcgttgcga aaaaccgctt gatacgcttc acggaaaata ccgttgtctt tattattgac
      721 ccgcgaggcc agcacattgc cttcgctcca taacacgtta cagccaaaat taaacgacga
      781 accgaatttg ggttgacgtt tttcgaatac cacgatatcc gtcgaaccgc cgccgatatc
      841 cacgcacagt acattgctgt caccgacgac cgcggtcgtc gctttgtgat aataatacgg
      901 tgcttgcgac tccgtcattt ctattacggc ttgttcggaa atgcccagca ctttttgagc
      961 taattgcgtc caatcgcttt tgagcaatcc gatcgcatgc gcgctcatac tcagcgggtg
     1021 aaaccatatg agtttggttt tggccggatc gccctgattg agaacgactt tgtggcgaat
     1081 cagaagtaaa atttcttcaa aaaacgcacg gatccgttcc gtggtttttt cgtcgagatt
     1141 gattttccat ttgatattgg tgtgtacgat ttcgtcgtca aactccacat gccgctcgtg
     1201 gttaaacgcc atattgatat taccgagcgt aaacgtctct tcgcgtacaa atccgggcga
     1261 ttcgctcgtc gccgtacgaa tcggaaactg gatcggagaa ccggcagcac cgataaacgt
     1321 cggcacaaaa tcgttttcct gatataccga ggattgcggc aatacggtgc gatcgttgta
     1381 gcggcgatat aaatcggtca ccgcatcacg attggaaatt ttttcaggtc cgtacaaatt
     1441 gaccaccggc atatcttctt tggtaaaatc aaaaggtttc ggagcgcccg tggcatctgc
     1501 atacgcgatg tgcgtagacg ttgtaccaaa atccacggca aacgtatatg cccgcgttcc
     1561 cggctcgacg taacgccaca tcggcacgat ccatcctacg gctttttgcg gacctttgag
     1621 ttcgcagcga atataatcaa attgagcacc cgtaatctga taatagacgc cgccgtcaaa
     1681 cactttttta tcatggcgta caatcttctt cacgggcgat tgtttgcttt tactttccga
     1741 cagcggctcc atgcggctgt caaaaaaagt caaatccgga aaatcttttc ggtttcgctg
     1801 cccgtccacc atcatgacat tgtaccgctc gacggattcc gcttcctgtt tcacacgata
     1861 aaaaggaaaa accgctacgt caaatttcca ctccacgata cgtccggtgt ccgtgccttc
     1921 gatcggatcg ggtacatacc ggcgtttcag ttccacgcgt ttgcccgaac gggtcggtat
     1981 ctgcaaccgc acggtcacgg catctccgtc aaccgtaaac gaaatttgtt tcctaaggtc
     2041 ttccggcgaa aaatagttat aataaaataa ttttaccgga agcatatacg attcgtcttc
     2101 atcgctgtgc gtgagcgtca aaaacgaagg atgcgctttg tacggtaact tgatcagcga
     2161 attttccaat aaatccgaag ccaacaaata cggatatggt atctgcgtac cgggcaacgt
     2221 tcgactttcc aaaggattcg gatccgtcac cggtacttgg cgacgcacct cttcattcca
     2281 gtccgcatcg acgtagatca cacccggatc ggtaaattgt ttggacaata ccatcggtat
     2341 caaaccgtct gtgggtttgg tcacatccat cagaaagccg ctgcgctcgg atatccggtt
     2401 agtcaaatcc cgcgcacatc gccatccgac ctgatgcaag taaagtttca gatttttggg
     2461 ctcggtgtat tcggaacgaa aatcatgttc ggaaaaaccg ttggcataaa tcgcatccac
     2521 ggcgatgcgc agttcgcgat ctttgatcca tggtcggttt ttatccacat agtcgtgaac
     2581 cgaacgatag atacctgaaa attcacgatg ccccgctatc agacggtgta cgaacatctg
     2641 aaaatcgcga tcccgttcgt gcagcgccaa cgttgtttca aaatagcgtg tgccgttggt
     2701 gcggcgaata tcaatcgttt tgagcgtagc atgatgcggc acggtaaaaa acatagtcaa
     2761 cggcgatgtc gccgcaaaaa cacgatggtt gtacgcgatc agataaaacc ggtcgaagct
     2821 gccaaaatta gcggaatcgc gatcctgttc gataaacagt ttgagtgttt cacccaaaat
     2881 gcgatgacca tcggaacgcg atgtgatgag tccgtcaatc tgatgattaa aaacccattc
     2941 t
//
LOCUS       DAOQNQ010000362         2920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_18177_length_2920_cov_5.312391, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000362 DAOQNQ010000000
VERSION     DAOQNQ010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18177_length_2920_cov_5.312391"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="PK754_14900"
     CDS             complement(<1..970)
                     /locus_tag="PK754_14900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB10839.1"
                     /translation="MSLKRLPHSQDTGDSNTLNHHEILVVDDEENVLSVLSRLLSDEY
                     KVHTATNGKDGLEILEKNPNIALIISDQRMPVMSGTEFLNRSISVNPDCIRIILTGYT
                     DVKDLIESINAGRVFQYVTKPFETDDLRIIVKRSLDFFAQSRALERAHLEIKTAYENL
                     KNAQEQLVRSEKMSMLGKLMSSIAHEIRNPINNISNSTKLISLEWADVKNLFIEVNAL
                     TTGQKTAEQFKSAVKDRYNIEEVLQNFETAVGVIKHSCDLVTEIVEDLRGFSRLDDAE
                     FVQTDIHQQIERSLTLLKAKFKHHIEFVKDFGVVSHILGLPGPLSQV"
     gene            complement(1041..1877)
                     /locus_tag="PK754_14905"
     CDS             complement(1041..1877)
                     /locus_tag="PK754_14905"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNB10840.1"
                     /translation="MKRLIWRIEYFILLFFRYVLLVLPLPLIPYIGALIGDVFHFFKI
                     RHNVAYSNLVRTGLYDPSAIQRILRQTYRQFGKTFAELLVLDRYNDAHAKQFSFRLPE
                     DWDWHNLRSSVLISAHFGNWELMGKALIAHGVPLAVVVRRQTNPYVDAWLFRHRTRCG
                     MKVLYEDDIYGILRTLRSGHALALLSDQDFGHNKIPVNFFSQPCFAPAGPEMLARRTG
                     CRAWICLGKRETSDQFVFSIDTISAPETSFSSHYTALIESAIRSAPEQWFWFHRRWKN
                     NK"
     gene            complement(1874..2326)
                     /gene="rnhA"
                     /locus_tag="PK754_14910"
     CDS             complement(1874..2326)
                     /gene="rnhA"
                     /locus_tag="PK754_14910"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNB10841.1"
                     /translation="MTTSQSPHITIYTDGACKGNPGRGAWAAVLLAEDGKSKEISGTE
                     DATTNNRMELAAVAEALKALKRPCRVTLHSDSAYIVNAFHQKWLDNWQRNGWRTADKS
                     PVKNRDLWETIINLCQTHTVRFEKVKGHAGIHYNERVDTLCNEALSVQ"
     gene            2651..>2920
                     /locus_tag="PK754_14915"
     CDS             2651..>2920
                     /locus_tag="PK754_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10842.1"
                     /translation="MKRLTVLIGFVCCPILSLFAQYQIDATESVHVPVLIKSLSYDSK
                     LKGGNLLILSRTDENSNRSKASSMFSGKTIKSNTLQAFDGTYSGKA"
BASE COUNT          775 a          620 c          704 g          821 t
ORIGIN      
        1 atacttggga tagcggcccc ggtaatccca gaatgtgcga tacgacgcca aaatccttta
       61 caaattcgat gtgatgcttg aattttgctt tcagaagtgt gagtgaacgt tcgatctgct
      121 ggtgtatatc ggtttgaaca aattcggcat catcaagccg tgaaaatccg cgcaaatctt
      181 cgacaatttc tgtaaccaga tcacacgagt gtttgatcac accgactgcc gtttcaaaat
      241 tttgcagtac ttcttcgatg ttataacgat ccttgacggc cgacttaaat tgttcggctg
      301 tcttttgacc ggtcgtgagc gcgttgactt cgataaaaag atttttgacg tccgcccatt
      361 ccagcgaaat caatttggtc gaattgctga tattgtttat cgggttgcgg atctcgtggg
      421 caatgctgct catgagtttg cccaacatgg acattttttc ggagcgaacc agttgctcct
      481 gagcattttt cagattttca taagccgttt tgatttcaag atgagcccgt tcgagagccc
      541 ggctttgggc aaaaaaatcc aggctgcgct tgacgataat gcgcagatca tcggtttcaa
      601 agggtttggt cacatactgg aatacgcgcc ccgcattgat ggattcgatc aaatctttga
      661 catcggtata accggtgaga ataatgcgga tgcagtcggg attaacgctg atagaacggt
      721 tgagaaattc ggttccggac ataaccggca ttcgttgatc ggaaattatc agcgcgatat
      781 tgggattttt ctcaaggatt tcgaggccgt cttttccgtt ggtggccgtg tgaactttat
      841 attcatcact caaaaggcgc gataacacag ataatacatt ttcttcatca tccacgacca
      901 ggatttcgtg atgattcagc gtattgctgt ctccggtatc ctgcgaatgc ggaagacgtt
      961 tgagcgacat aggattgtat tgagatggtg gcttgaaatt tgaaaaaaat acgttctgag
     1021 tgagtttaaa tcaagaataa tcatttattg tttttccagc gacggtgaaa ccaaaaccat
     1081 tgttcgggag cggaacggat cgccgattcg atcaatgcgg tatagtgtga tgagaatgat
     1141 gtttccggcg cagaaatggt atcaattgaa aatacaaatt gatccgaagt ttctcgcttt
     1201 cctagacaaa tccaggcccg gcagcccgtg cgtcgggcga gcatttcagg gccggccggt
     1261 gcaaaacacg gttgagaaaa aaaattcaca ggtattttat tgtgaccgaa atcctgatcc
     1321 gaaagcaaag ccaaagcgtg accggatcga agtgttctga gtatgccgta aatatcatct
     1381 tcgtacaaaa ctttcatgcc acaacgtgtg cgatgtcgaa atagccatgc atccacgtac
     1441 gggttcgttt gacggcgcac taccaccgcc agcggaacac catgcgcgat caacgctttt
     1501 cccatcaatt cccaattccc aaaatgggcg gagatcaaaa ccgagctgcg caagttgtgc
     1561 caatcccaat cttccggtaa gcggaatgaa aattgtttag catgagcgtc attgtaacgg
     1621 tccaatacta agagctctgc aaacgttttg ccgaattggc ggtatgtttg cctgagaatg
     1681 cgttgtatcg cagacggatc atacaaaccc gtgcgtacca gattagaata cgctacgtta
     1741 tgacggattt taaaaaaatg aaatacgtca ccgatgaggg cgccgatgta tggaatgaga
     1801 gggagtggaa gaactaaaag aacatagcga aagaaaagca ggatgaaata ttcgatccgc
     1861 cagatcaacc gtttcattga accgacaagg cttcgttgca caacgtgtcc acgcgttcat
     1921 tatagtggat gccggcatgg cctttgactt tttcaaagcg tacggtatgc gtttggcata
     1981 gattgatgat agtttcccaa aggtcacggt ttttaacagg gcttttgtcg gcggtgcgcc
     2041 agccgttgcg ttgccagtta tccagccatt tttgatggaa ggcgttgacg atgtaagcgc
     2101 tgtcggaatg caacgtgacc cggcaaggac gtttgagcgc tttaagcgct tcggcgacgg
     2161 ccgccaattc catccggtta ttggtcgtgg cgtcttccgt tccggaaatt tctttacttt
     2221 ttccatcctc ggcgagcaaa acagccgccc atgcgccgcg cccggggtta cccttgcagg
     2281 cgccgtcggt gtatatcgtg atatgtgggc tttgtgatgt tgtcatagct acattttata
     2341 aaaattatca tcaaccaaaa gatttttttt taaaataaga tcatatctgc attggacttg
     2401 accttgaaac gcccgatgct cccgttttct taaatggtta tcgcttacac gcaaaagcag
     2461 gccgttgaag atcggtattt tgtaagaaca tcgcagggcg gatgcagaac ggatagatat
     2521 aaaaatacga gttgtgttga atagtttcgg cagtaatatt tcttgcgatt acacttcatt
     2581 tttctttaca ttggggcggt tccgtcttat cacttatcaa ataaaaaaaa aacctttttt
     2641 ggaggatcgt gtgaaacgat tgaccgtact tatcggtttc gtgtgttgtc ctattttgag
     2701 tttgtttgct caatatcaaa ttgatgccac agaatccgtg catgttcccg tgctgatcaa
     2761 aagtttatcg tatgatagta agttgaaagg cggaaatctt ctgatattgt cgcgtaccga
     2821 tgaaaatagt aatcgcagta aagcgtcttc gatgttttcc ggaaaaacga tcaagtccaa
     2881 cacattgcag gcgtttgacg ggacgtattc cggtaaagca
//
LOCUS       DAOQNQ010000363         2902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_18364_length_2902_cov_5.195645, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000363 DAOQNQ010000000
VERSION     DAOQNQ010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18364_length_2902_cov_5.195645"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..986)
                     /locus_tag="PK754_14920"
     CDS             complement(207..986)
                     /locus_tag="PK754_14920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB10843.1"
                     /translation="MIRPSDFANWQEYYFTYQNILAKQYLIPMLEQWGVSIDGKKILE
                     IGCGSGGVIEAFAERASKAVGVELNPFDYTKLNTPKVQYVTADIYDPSHRERYADKYD
                     IILFRDVIEHLPKKKEAFEICDTLLSENGVIFITFPPYHGPYGAHQQVFSKTFLGRLP
                     YTHLLPNSLYIKYVKAVEKGNDAALQVAHELIASQTTIGSLKKSIRASAFKIDRYDYY
                     LVRPSYEIRYGKKPRKINLMKYLPFIREFFVLGMYMIMRRK"
     gene            complement(983..2605)
                     /locus_tag="PK754_14925"
     CDS             complement(983..2605)
                     /locus_tag="PK754_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10844.1"
                     /translation="MKNKESKIKNTSPLKSSGLADWNQTKTWLILSLSTVVLTLIYYM
                     VNSPRPYDDDNIGRYFMAQAAFVKPGYFLDIWGRPMAILLFSIPAQFGYWYVASGVAL
                     LTVMTCFFTYKAAQESGRPYAWMVVLFVFFQPLVLTTSFSLCAEPLAAFFVAIALYFY
                     YRNKLWQAALLFSMVPLARSETVLILPLIAIPWLRERRWIPVLLLGTGLAVYQIAGMI
                     STGDPLYLLTQSKAFGHGLYQNGPFEHYFQRFIFITGPVIFVFLLFQLASDIRLRKLS
                     LLNTSVVLMFAVHVYFYWKGNVASVGFLRHFVAVGPMIALLALDGFNRWIGFEDKQKT
                     NSVVWDAFILAALSVLILAYYSFELVGQYFLSDKAEHLKFGTALVLFLLFILRAVLKL
                     DGIFFKKIMITGTVILTVAYCFVKEKPLTFAPEHQTVKNCYDYYKENIRGKTPKVMIA
                     HTWWFFFDNFNNYSKTLEEGGYENMRKEKLADLPVGSFVIWDSHYSHRLSSNVQLRED
                     LAGDKNFQLKQQFLSSDQKFVIYIFEKVKQPS"
     gene            complement(2623..>2902)
                     /locus_tag="PK754_14930"
     CDS             complement(2623..>2902)
                     /locus_tag="PK754_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10845.1"
                     /translation="TEFLYDETDANYWQWQKDSDRRKYDKMRADTKRFRQVSTYMYFG
                     LFVNHVMSIVHVTRQYKRMTTAQSTTGWRFYIEPSSTGTACIQLVKTF"
BASE COUNT          947 a          635 c          529 g          791 t
ORIGIN      
        1 ggatcgaatc aaatacaata tctacaacgt gttccacttc gtgaattttt tgaccaagct
       61 tatcagctac acgtttggaa acatcttttt tggtcatagt tttcattgga gaatctccta
      121 atgcgcacgc aaagctaaaa caaataaaat caactaaatt cgcccaatta taaaataaat
      181 aactaattaa gtcaagtaaa atcaggtcat tttcttcgca tgatcatgta catgcccaaa
      241 acaaaaaact cacggatgaa tggtaagtac ttcattaaat ttattttacg tggttttttt
      301 ccatagcgaa tttcatagga tggccgcacc aaataataat cataacgatc aattttgaaa
      361 gccgatgcgc gaatggattt tttcagggaa ccgattgtag tttgacttgc gatcagttca
      421 tgtgctactt gcaatgccgc atcattgccc ttttccaccg ctttcacgta cttgatatac
      481 aatgagttag gtaataaatg cgtatagggt aatcttccca aaaaagtttt agagaaaacc
      541 tgctggtgtg caccataagg cccgtgatac ggcggaaatg taataaaaat aacgccgttt
      601 tcacttaaca gagtatcaca tatctcaaat gcttctttct ttttaggcaa atgttcgatc
      661 acgtcacgaa atagtataat atcatattta tcggcatacc gttcacgatg tgaaggatca
      721 tagatatcgg cggtaacgta ttgtactttg ggagtattga gtttggtata atcaaaagga
      781 tttaactcca caccgaccgc tttcgatgca cgttcggcaa acgcctcgat caccccaccg
      841 cttccacagc cgatctccaa aattttttta ccgtcaatgg aaacgcccca ttgctccagc
      901 ataggaatga gatattgttt ggctaatatg ttctgatacg taaaataata ttcctgccaa
      961 ttggcgaaat cggatggacg aatcatgacg gttgttttac cttttcaaaa atgtagatga
     1021 caaatttttg atcggacgat aggaattgct gcttgagttg aaaattctta tcacccgcca
     1081 gatcttcgcg gagctgaaca ttggaactca aacgatgact atagtgcgaa tcccaaatta
     1141 cgaagcttcc gacgggtaaa tccgcaagtt tctcttttcg catgttttca taaccgccct
     1201 cttccaatgt ttttgaatag ttattaaaat tatcaaagaa aaaccaccat gtgtgtgcta
     1261 tcatgacttt gggtgtctta ccacggatgt tctctttata atagtcataa caatttttta
     1321 cggtctgatg ctcgggagca aacgtaagcg gtttttcttt aacaaaacaa taggctaccg
     1381 ttagtatgac cgtcccggtg atcattattt ttttaaagaa aatcccgtct aatttaagta
     1441 ccgctcttaa aataaataac aaaaacaaaa caagcgctgt tccaaattta aggtgttcgg
     1501 ctttatccga taaaaaatac tggccaacca attcaaacga gtagtacgcc aaaatcaaaa
     1561 cgcttagtgc cgctaatata aatgcatccc aaacgactga atttgttttt tgtttatcct
     1621 caaatccgat ccaacgattg aaaccgtcca aagctaaaag cgcgatcatg ggaccgacag
     1681 ctacaaaatg acgtaaaaaa ccgaccgatg ccacattccc tttccagtag aaatatacat
     1741 gcaccgcaaa catcaatacc acagaagtat taagtagaga cagcttgcgt aatcttatat
     1801 ccgaggccaa ctggaacaaa agaaaaacaa aaatcacagg acccgtaata aaaataaatc
     1861 gctgaaaata atgttcaaaa ggtccgtttt gatataaccc gtgcccaaaa gctttggatt
     1921 gggtcaacag atacagcggg tctcccgtcg aaatcatacc ggcgatttga taaacggcta
     1981 aacccgtacc aagcagcaat accggtatcc atcggcgttc acgcagccag ggaatcgcta
     2041 tcaaaggaag tataagcacc gtctcggaac gcgccagggg caccatcgag aaaagtaaag
     2101 cagcttgcca taacttattg cgataataaa aatataatgc gatggcaaca aaaaaagccg
     2161 ccagcggttc ggcgcataaa ctaaatgatg tcgttaagac caacggttga aaaaaaacaa
     2221 aaagtacaac catccaagca tagggccgtc cactttcctg cgcggcctta taggtaaaaa
     2281 aacatgtcat cacggtcaaa agagctaccc cggaagcgac ataccaatac ccaaactgag
     2341 ccggaatcga aaaaagtaaa atagccatcg ggcgccccca gatatccaaa aaatatccgg
     2401 gcttcacaaa cgctgcctgc gccataaaat aacgccctat gttatcatca tcatacgggc
     2461 gcggtgaatt gaccatataa tagataagtg tcaaaacaac ggttgacaga gataatatca
     2521 accatgtttt cgtttgattc caatccgcca atccggacga tttcaatgga gatgtatttt
     2581 tgattttact ttctttattt ttcataacat gttttaagtg aattaaaaag ttttgactaa
     2641 ttgaatgcag gccgttcctg tgctcgacgg ttcaatataa aatctccatc ctgtcgtgct
     2701 ttgagccgtg gtcatgcgct tatattgacg cgtcacatgt acgatactca tcacatgatt
     2761 tacaaacaaa ccaaaataca tatatgtaga aacctgacga aatcgcttcg tatcggcgcg
     2821 catcttatcg tacttccgac gatcagaatc tttttgccac tgccaatagt tggcatcggt
     2881 ctcatcatat aaaaactccg tc
//
LOCUS       DAOQNQ010000364         2889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_18519_length_2889_cov_5.022230, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000364 DAOQNQ010000000
VERSION     DAOQNQ010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18519_length_2889_cov_5.022230"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PK754_14935"
     CDS             complement(<1..406)
                     /locus_tag="PK754_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNB10846.1"
                     /translation="MARTPSNMASLGTFAPDFDLPDVISEKKLKLADFKEKPALLVMF
                     ISRHCPFVVHVEKELARIGKDYPQIGIVAICSNDIENYPEDAPDQLREQANRLQFTFP
                     YLYDASQETAKNYKAACTPDFFLYDQSRRLIYR"
     gene            complement(1080..1985)
                     /locus_tag="PK754_14940"
     CDS             complement(1080..1985)
                     /locus_tag="PK754_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNB10847.1"
                     /translation="MSIRPVKRIIQSTPTMEGAGVRLRRAFGFGNTEDYDPFLMLDDF
                     RNDNPNDYMAGFPWHPHRGIETITYVLAGNVDHKDSLGNAGSLGAGDVQWMTAGSGIL
                     HQEMPQGDAQGRMHGFQLWANLPSSLKMTGPRYQDVKSKDIPEISEDEGTKIRVVCGD
                     YGGQKGPVDGIAADPRYLDVFVPSGKRKTLKVETYRQAFAYIFEGSGSFRDASQPHGI
                     LTEAPDDHDNVSRSMAGNRSLVLFDSGDEVTVQAGDEGIRFLLVSGRPIKEPVAWYGP
                     IVMNTNAELQQAVSELRNGTFIKHG"
     gene            complement(2034..2624)
                     /locus_tag="PK754_14945"
     CDS             complement(2034..2624)
                     /locus_tag="PK754_14945"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HNB10848.1"
                     /translation="MTSFLLYIIFVYLVGGLCGAYYLLHWKTGENLRDHGSGNLGATN
                     AGRVLGKTGFIITFLWDAGKGSAVIYGARILDYDQHLIWICAIAVVTGHIWPIQLQFK
                     GGKGAATLIGILVALDAYLLWPLLFIFLASFVILRRFGIAGALAFFTLPLVVWLRGYE
                     PLSVILSIILTIIVIFAYRDHIVTFVSAFKKRSRNK"
     gene            complement(2670..>2889)
                     /locus_tag="PK754_14950"
     CDS             complement(2670..>2889)
                     /locus_tag="PK754_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB10849.1"
                     /translation="NELERKNFYDFVNQYRLNHFIKLISDTRNRYLTFLALAYESGFN
                     SKSSFNRYFKKATGKTPSEYFSSEGLVN"
BASE COUNT          813 a          643 c          611 g          822 t
ORIGIN      
        1 ccctgtaaat caaacggcgc gattgatcat ataaaaagaa atccggagta caagctgctt
       61 tataattctt agcggtctct tgcgaggcat cgtacaaata aggaaaagta aattgtaacc
      121 ggttagcttg ttcacgtaat tgatccggcg cgtcttcagg ataattttcg atatcgttgg
      181 aacaaatggc tacaatgccg atctgcgggt aatcttttcc gatacgcgct aattcttttt
      241 cgacatgcac tacaaaaggg caatgcctgc tgataaacat aacaagcaaa gccggttttt
      301 ctttaaaatc agccagtttt aatttttttt cagagataac gtcaggcagg tcaaaatcag
      361 gagcaaatgt cccgagcgag gccatgttcg aaggtgtgcg cgccatacta atgaattagg
      421 aaataatatg atatgttggt gcaaaataca gccgctttga gcgcgtggca actacttttt
      481 tgaaggacgg cgtaaaagtg caatagggcg cttattttct gaatccttga tgaggatatc
      541 aagacgcttt ttttgtgtgg catcctgttt ggcgcttacc acccaccaaa tcgccgcttt
      601 gcgatacgac ggtgatttca attccaagta atcccacgct atggtatcgg tttgaaagat
      661 cgaaatgagg gcatccggta aatccgcatt tgctcttccg taggaatatg gtttagattt
      721 ttttatatcg cgtttttgaa agcttccaaa ccataaggat ataccattct ttttttgtgg
      781 agttctctaa atctctttgt attccgctcc agatgctacc cggcttacgt ggtgtaaagc
      841 gattctgaaa cgcttcggcg ttaatcttat gccgaatgct gtctatccat ccgaaacaca
      901 aagcctgatc tactgaatcc tgccaagtaa tgcttggctt ccccgtagct tttttgtaat
      961 aacctacgac taattcgtcg gcgaagccgt gatatttttc gaaccaacgt cgtaagtttt
     1021 ctgatgttgg aaaaaaatag gttttgacac cggcttagcc atgaatgttt taaaataaat
     1081 tatccgtgtt tgataaaagt tccgtttcgt aattcggaaa cggcttgttg aagctcggcg
     1141 ttggtattca tcacgatcgg gccataccac gcgacgggtt ctttgatcgg tcgcccggaa
     1201 accaataaaa atcgaatacc ttcatcgccc gcttgtacgg tgacttcgtc accggagtca
     1261 aaaagcacca atgaacgatt tccggccatg gagcgcgaca cattatcatg gtcatccggc
     1321 gcctcagtca aaatgccgtg cggctgtgaa gcatcgcgaa aagaaccgga gccctcaaat
     1381 atatatgcaa acgcttgtcg gtaagtttct acttttaaag tcttacgttt tcctgacgga
     1441 acaaacacat caagataccg cggatctgcg gctataccgt ccacaggtcc tttctgtcct
     1501 ccgtagtcgc cgcatacaac acgtattttt gtaccttcat cttctgaaat ctccgggatg
     1561 tctttacttt tgacatcttg gtatcgcgga cctgtcattt tgagcgaaga aggaagattg
     1621 gcccacaact gaaaaccgtg cattcgccct tgtgcatcgc cttggggcat ttcctgatgt
     1681 aaaatgccgc tgccggctgt catccactgc acatcacctg cgccaagcga acccgcattg
     1741 cctaaactgt ctttgtgatc tacattaccg gctaatacat aagtaatggt ttctatacca
     1801 cgatgcggat gccacggaaa acctgccata taatcattag gattatcgtt tctgaaatcg
     1861 tctaacatca aaaaaggatc ataatcctcc gtattaccaa aaccgaaagc gcgtcgtagc
     1921 cttacaccgg caccttccat cgtaggtgta gactgaataa tgcgtttgac gggacgtatg
     1981 gacatagctt ctcctaaaat ttgagcaact tattttataa tatagatagc ggcttattta
     2041 ttccggcttc tttttttaaa ggcacttaca aaagtaacga tatgatcgcg gtatgcgaaa
     2101 atgacaatga tcgtcagaat gatactgagt attacagaga gaggttcata gcctcgcagc
     2161 catacgacga gtggtaaagt aaagaaggcc agagcaccgg ctataccgaa tcttcgtaga
     2221 atcacaaaag atgccagaaa aataaaaagt aatggccaca gcaaatatgc gtccagtgct
     2281 actaatattc caatcagtgt agcagcacct ttgcctcctt taaattgcaa ttggatcggc
     2341 caaatatggc cggtcactac ggcgatggca caaatccata taaggtgttg atcataatcc
     2401 aaaattcgag ccccgtaaat gaccgctgag cccttaccgg catcccataa aaaagtaata
     2461 ataaagcctg ttttgccaag cactcttccg gcgttcgttg cacccaggtt tccactcccg
     2521 tgatcgcgga ggttttcgcc tgttttccag tgtagtagat aatacgcccc acacagaccc
     2581 ccgacgagat aaacgaatat gatgtaaaga agaaaagagg tcattcgatg tttggtgaat
     2641 atttgttaaa ttcgttttct gattcagagt taattcacta gtccttcgct cgaaaaatac
     2701 tctgacggcg ttttgccggt cgcctttttg aaatatcgat tgaatgaaga tttagaattg
     2761 aaaccgcttt cataggctag agcaagaaac gtcaaataac ggtttcgtgt gtcggatatc
     2821 agtttgataa aatgatttaa gcgatattgg ttgacgaaat cataaaaatt ttttctttca
     2881 agttcgttg
//
LOCUS       DAOQNQ010000365         2861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_18876_length_2861_cov_4.173913, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000365 DAOQNQ010000000
VERSION     DAOQNQ010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18876_length_2861_cov_4.173913"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PK754_14955"
     CDS             <1..745
                     /locus_tag="PK754_14955"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HNB10850.1"
                     /translation="TIIHESAVIRGVKGKAQRIIIGDNVYIGANVQIICDHFRLGDYS
                     KIHHGTNVHGYMPCQIGHNAWIGQYSIIDSIGGTTIGDNCGIGAHSQLWSHIKYGDTL
                     EGSRFKSESPLRIGKDVWFVGHCIVSPITAHDKSMALVGSVVTRDMEPNRMYAGSPAK
                     DITEKAGAPFQYIDIEQKYKRMMELLNESGVSKDMIKIVTSRSEIQDDGRSYFCIETR
                     EYTKTLNPEEIKFMNFLLPDKAKFTPIAK"
     gene            755..1879
                     /gene="rffA"
                     /locus_tag="PK754_14960"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             755..1879
                     /gene="rffA"
                     /locus_tag="PK754_14960"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HNB10851.1"
                     /translation="MFIPFNKPFLTGKELEYIRQSVESGKISGDGVFTKKCHAFFEQR
                     YGFKKCLLTTSCTDALEMAAILIEAGPGDEIILPSFTFVSTANAFALRGAKLIFADSE
                     ASTPNIDATKIEPLITSRTKAIVVVHYAGVACDMNTIMETAKKHNIFVIEDAAQAIDS
                     YYEGCPLGSIGHLATFSFHETKNVIAGEGGMLVINDERFIKRAEIIREKGTNRSAFFR
                     GEIDKYGWVDIGSSFLPSDIIAAFLYAQLEHIDEIQKKRKGLWNHYYTLLTFLEDKHR
                     VKLPFIPQYATNNAHMFYLVFSDTATRTTVIDLLKQNNIHAVFHYQSLHVSAFYKHNN
                     DGRDLPLSDRYTNTLLRLPMYYDLEREDVELIAKIIKSIA"
     gene            1897..>2861
                     /locus_tag="PK754_14965"
     CDS             1897..>2861
                     /locus_tag="PK754_14965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB10852.1"
                     /translation="MNKDQKITFLMPTFFSERVGGVEIQTYLLATHLSKLEWKVDFVS
                     ETYDVRKIGRSEIFDGVTIHWVSKKNHFSFWRRDIQKLVLLIKPDYIYQRGRSRFTSS
                     PIAINVAKKLKAKLIYHCAEDNDFNRSFNFEQVHQSTKSFFKKIILGFEALLSDYYFY
                     QTIKKSHLIIAQTESQKLQFLRFFNKDATVIRSAHELPNEVIQKSEPPMIFWIANTGR
                     RKQPEKFIELAKSLLDTPYRFVMAGPIPDRGYAEELLNQIRSTPNLEYAGPLSWEESN
                     NFFAKATFFVNTTLLGREGFPNTYIQAWMRGTPVLTLHCDPDGLI"
BASE COUNT          879 a          578 c          558 g          846 t
ORIGIN      
        1 cacaattatt catgaaagtg ccgttattcg cggtgtaaag ggtaaagctc agcgaatcat
       61 tataggtgat aatgtataca tcggcgccaa tgtccaaatt atttgtgacc atttcagatt
      121 aggagattat tcgaaaattc atcatggcac taacgtacac gggtatatgc cgtgtcagat
      181 cggacataat gcttggatcg gacaatattc tatcatcgat agtatcggag gcacgaccat
      241 aggcgataat tgcgggattg gcgcccattc acaactttgg agtcatatta aatacggtga
      301 tactttggaa ggttcacgct tcaaatcaga aagtccattg cgaattggaa aagatgtatg
      361 gttcgtaggt cactgtatcg tttcccccat tacagcgcat gataaatcaa tggctttggt
      421 gggttctgtg gtaacaagag atatggaacc caatcggatg tatgcaggta gccccgccaa
      481 agatattaca gaaaaggccg gcgccccttt ccaatatatt gatatcgaac agaaatataa
      541 aagaatgatg gaattattga atgaatccgg ggtaagtaaa gatatgatta aaattgtaac
      601 atctcgcagt gaaatacagg acgacggcag atcttatttc tgtattgaaa cacgagaata
      661 cactaaaact ttaaacccag aagaaataaa attcatgaat tttctgcttc cggataaagc
      721 caaatttact cctattgcaa agtgaatcaa caatatgttt attccattta acaaaccttt
      781 tctcacaggc aaagaactgg aatatattcg ccaatccgta gaatctggaa aaatttccgg
      841 agacggtgtt ttcaccaaga aatgccatgc tttttttgaa caacggtacg ggttcaaaaa
      901 atgtctgctc accacatctt gtactgatgc acttgagatg gcggctattc ttatcgaggc
      961 cggaccgggt gatgagatta ttttgccttc gtttactttt gtatctaccg ccaatgcctt
     1021 cgcattacgt ggcgccaaat tgatttttgc cgatagcgaa gcttctactc ctaatatcga
     1081 tgcgacaaaa atcgaaccgc taatcacctc gcgcacaaaa gccatcgttg tcgttcacta
     1141 tgcaggtgta gcctgcgata tgaatactat tatggagaca gcaaaaaaac acaatatttt
     1201 cgtcatcgaa gacgccgccc aagcgattga ttcttattac gaagggtgcc cgttaggttc
     1261 catcggacat cttgcgactt tttcatttca tgaaactaaa aatgttattg ccggcgaagg
     1321 tggtatgctg gttatcaatg atgaacgttt tataaagcgc gctgaaatca ttcgtgaaaa
     1381 aggtacaaac cgctcagctt tttttcgtgg tgaaattgat aaatacggat gggttgatat
     1441 tggttcttca tttctccctt cggacatcat tgccgcattt ttatatgctc aactcgagca
     1501 catagatgaa atccaaaaaa agagaaaagg tttatggaat cattattaca cactactaac
     1561 gtttttggaa gacaagcatc gcgttaaatt accatttatc ccccaatatg ccacaaataa
     1621 tgcgcatatg ttttatttgg ttttttctga tacggcgacg cgtacgaccg tgatcgattt
     1681 actaaagcaa aacaatatcc atgcggtatt tcattaccaa agcctgcacg tttctgcatt
     1741 ctataaacac aacaacgatg ggcgtgacct cccccttagc gaccgttaca cgaatacgct
     1801 gcttcgttta cccatgtatt atgatctgga gcgtgaggat gtcgagctaa ttgcgaaaat
     1861 tatcaaatct attgcctaat aacattcaca acctcaatga ataaagatca gaaaataact
     1921 tttctcatgc ctactttttt ttctgagcgc gtgggtgggg ttgaaataca aacttattta
     1981 ttggcgactc acctttccaa gttggaatgg aaagtagact ttgttagtga aacatacgat
     2041 gtacgtaaga ttggaagatc ggaaatattt gatggagtga caatacattg ggttagtaaa
     2101 aaaaatcatt tttccttttg gcgccgcgat attcaaaaac tcgtactttt aataaaacca
     2161 gattatatat accaacgtgg ccgctctcgc ttcacctcct cgcctattgc cattaatgtt
     2221 gcaaaaaagt tgaaagcaaa acttatttac cattgcgctg aagacaatga ctttaataga
     2281 agctttaatt ttgaacaagt gcatcagagt acaaaatcat ttttcaaaaa aattattctc
     2341 ggttttgaag ccttactttc agattattac ttttatcaaa ccattaagaa atctcattta
     2401 attatagccc aaacagaatc acagaaactt caatttttac gtttctttaa taaagacgcc
     2461 accgttatcc gttccgctca tgagttacct aatgaggtga tacaaaaatc agaaccaccc
     2521 atgatttttt ggatagccaa tactggccgt cgcaaacaac cggaaaaatt cattgagctt
     2581 gccaagtcgc tcttagatac accgtatcgt tttgtaatgg ccggtccgat tcctgatcga
     2641 ggatatgccg aagaattatt aaatcaaatt cgatctacac ccaacttgga atatgcggga
     2701 ccgctttcgt gggaagaaag taataatttt tttgcgaagg ctactttttt tgtcaacact
     2761 actttgctcg gccgcgaggg ttttcccaat acttatattc aggcttggat gcgtggaaca
     2821 ccggttttga ccctccattg tgatccggac ggactgattg a
//
LOCUS       DAOQNQ010000366         2860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_18890_length_2860_cov_5.404278, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000366 DAOQNQ010000000
VERSION     DAOQNQ010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18890_length_2860_cov_5.404278"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PK754_14970"
     CDS             <1..508
                     /locus_tag="PK754_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HNB10853.1"
                     /translation="PIIGPRGVNKEGSGDMYPKGGLMLNTLRSVINNDKLWFEILRGI
                     QDTYRYKTIDADSIFNYVRLKTGTDYDYFFDQYLKHKALPKLDVALFKKGSTTTMRYR
                     WTADVKSFRMPIRVTTTKDRYEWVTPTTEWQMMKLENLDPRDFKIEEDRFYVDKNIYW
                     MFLDERLQ"
     gene            545..1186
                     /locus_tag="PK754_14975"
     CDS             545..1186
                     /locus_tag="PK754_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNB10854.1"
                     /translation="MYELIAFGLLCFTSFFSVINPLGVMPVFLTMTADLDAAHRNSTA
                     RKAVLVAVISMVGFALSGQLLFKFFGISVDSFRVVGGVIFFMMGYEMLQARLSRVEID
                     EESVKSYVEDISITPLGIPMICGPGAITTSIVLMEDAKTFEMKAVLLGVILLIGLITL
                     IGLLGAGRFVNILGETGNKILMRLMGLIIMVIAVEFFFAGIKPFVVRMMQTVG"
     gene            complement(1421..>2860)
                     /locus_tag="PK754_14980"
     CDS             complement(1421..>2860)
                     /locus_tag="PK754_14980"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNB10855.1"
                     /translation="DGDYTLTTKPFPEFQITDTAADRNRLPEAGVPPEVRFSKMERHT
                     LANGLKIILVPRHSVPVINMSLVLDAGYASDALSIPGTASLAMAMLDEGTTGRSSLEI
                     SESLAMLGSTLTTGSSLDVSTVNLSTLKNNLSPSLDIFADVICRPIFPEQDFLRLREQ
                     QRAGIRQEKSSPVPMALRVLPQLLYGPSHAYGIPFTGSGTESALDRITRDDLKRFHQT
                     WFHPNHTTLIVVGDTTADVIIPLIENKLGAWQSGTTPQKNVVPVKIPDAPVVYLMDRP
                     GSQQSTIFAAHIAPPTNNPDEIAIQTMNNILGGDFTSRINMNLREDKHWAYGAFSFLW
                     DARGQRPFIAYAPVQSDKTKESVAEIRKEISDFITIKPLSEEEFMRTQSNQVLQLPGT
                     WETNNAVLGSLQQLVQFGFNDDYFDHYAQSIRNLSPADVRKAAQSLLKPDHLIWVIVG
                     DRKQIENEIRSLNIGVIRYIDSDGNVLES"
BASE COUNT          731 a          619 c          721 g          789 t
ORIGIN      
        1 tccgatcatc ggcccgcggg gcgtgaataa agaaggctcc ggcgatatgt atcccaaagg
       61 tggactgatg ctcaatacgc tgcgcagcgt aatcaataat gataaacttt ggtttgaaat
      121 attacgcggt attcaggata cgtaccgtta caaaacgata gatgccgatt ctatcttcaa
      181 ttacgttcgc ctgaaaaccg gaacggatta cgattatttt tttgatcaat atctcaaaca
      241 caaggctctg cctaaactcg atgtcgcgct attcaaaaaa ggttcgacca cgacgatgcg
      301 ataccgctgg acggcggatg ttaaaagttt tcgtatgccc attcgtgtaa cgacaactaa
      361 agatcggtat gaatgggtca cgccgacgac ggaatggcag atgatgaaat tggaaaatct
      421 ggatccgaga gattttaaga tcgaagaaga tcgtttttat gtagataaaa atatctactg
      481 gatgtttttg gatgaacgct tgcaataaca tcttgtcata aattagagta aaggctattg
      541 ccgcatgtac gaactcatcg ctttcggtct tttatgtttt acatcttttt tttcggtgat
      601 taatccgctg ggtgtgatgc cggtttttct gacgatgacg gcggatctcg atgctgcgca
      661 tcggaacagt acggcgcgca aggctgtgct ggtggcggtt atcagtatgg tcggcttcgc
      721 tttgtcaggg caattgcttt tcaaattttt tggaatttcc gtggatagtt ttcgcgtggt
      781 cggcggcgtg atatttttta tgatgggata tgagatgctt caggcgcgtc tgagccgtgt
      841 cgaaattgac gaagagtcgg tcaaatcgta cgtggaagat atatcgatca caccgcttgg
      901 aattcccatg atctgtggcc ccggagcgat cacgacgtcc atcgttttaa tggaagatgc
      961 caagacgttt gaaatgaaag ctgttttgct cggcgtgata ttgctcatcg gcctgatcac
     1021 tttgatcggg cttttaggcg caggtcgctt tgtaaatatt ctcggtgaaa ccggaaataa
     1081 aatcctcatg cgcctgatgg gccttatcat catggttatc gccgtcgaat ttttcttcgc
     1141 cggtatcaaa ccgtttgtcg tacgcatgat gcaaacggtc ggataaggtt gccaggcgaa
     1201 agatgtaatc gaacctgcca ggacaatgcg gaaggtatca ttcttagccg tttgtggctg
     1261 agcttgggtt gagttatttt taactttgcg gctttgcgag agggcatacg tacgcgctaa
     1321 gacgctaagt acgcgaggcg tcatcaaaac aatttttatt cgcgagattt agtcgaacaa
     1381 tccctccgat ttgtactgag tcacgcggaa agtatcatac ctacgattct aaaacatttc
     1441 cgtcgctgtc gatatagcga ataacgccga tattgagaga acgaatttca ttttcgattt
     1501 gtttgcgatc gccgacgatc acccaaataa gatgatccgg tttcaataac gattgcgccg
     1561 cttttcgaac atccgcgggt gaaagatttc tgatcgattg tgcgtaatga tcaaaataat
     1621 catcattaaa tccgaactgg accaactgct gaagcgaacc taataccgcg ttattcgttt
     1681 cccaagtacc cggcagttgc agtacttgat tggattgtgt tctcataaac tcttcttcag
     1741 acagcggctt tatcgtaata aaatcggata tttcttttcg tatttccgcg acggattctt
     1801 tggttttatc gctttgtacc ggagcgtagg cgataaacgg acgttggccg cgcgcatccc
     1861 acagaaaact gaaggcgcca taagcccaat gcttatcctc gcgtaaattc atattgatac
     1921 gcgaagtaaa gtcaccgccg agaatattat tcatcgtttg aatggcgatt tcatccggat
     1981 tattagtcgg cggcgcgatg tgtgcggcaa aaatcgtgga ctgttgcgaa cccggccgat
     2041 ccatcagata taccacgggc gcatcgggta tctttaccgg cactacattt ttttgcggtg
     2101 tcgtgccgct ttgccatgcc cccaatttgt tttcaatcaa aggaattatg acatccgctg
     2161 tcgtatcgcc gaccacgatc aacgtggtat gattgggatg aaaccatgtt tgatgaaacc
     2221 gttttaaatc gtcacgcgtg atgcgatcaa gcgccgattc cgtaccggag cctgtaaaag
     2281 gtatgccata cgcgtgcgat ggtccgtaga gcaattgtgg taatacgcgc aaagccatcg
     2341 gcacgggtga agatttttcc tgacgaatac cggcgcgttg ctgttcacgg agacgaagga
     2401 agtcttgttc aggaaatata ggacggcaaa tcacatcggc aaaaatgtct aacgacggtg
     2461 agagattatt tttcaaagta gaaagattga cggtggagac atccagcgat gaaccggtcg
     2521 tcagcgtgct gcccaacatg gccaatgatt cgctgatttc gagagaagag cggccggttg
     2581 tgccttcatc aagcatggcc atagcgaggc ttgcggttcc gggaatggat aatgcgtccg
     2641 acgcatatcc cgcatcaagc acgaggctca tattgataac gggaacggaa tgccgtggta
     2701 ctaaaatgat tttaaggcca ttggccaaag tatgacgttc catttttgaa aaacgaactt
     2761 caggcggaac accggcttcc ggcagccgat tccgatctgc cgcggtgtcg gttatttgga
     2821 attccgggaa cggtttggtt gtcaaagtat aatcgccgtc
//
LOCUS       DAOQNQ010000367         2860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_18892_length_2860_cov_4.347950, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000367 DAOQNQ010000000
VERSION     DAOQNQ010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18892_length_2860_cov_4.347950"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..462
                     /locus_tag="PK754_14985"
     CDS             145..462
                     /locus_tag="PK754_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10856.1"
                     /translation="MNIQYDEIEISKPFVNEPGLDDDRTLILQTNNARRWLHKLIDEM
                     TLEQRFLLLDYAQSLITHQPHEETSSTLDSKINVLRAIEEAAKKRNEEPLANLISDIM
                     RPH"
     gene            535..1227
                     /locus_tag="PK754_14990"
     CDS             535..1227
                     /locus_tag="PK754_14990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB10857.1"
                     /translation="MVLKVAVTGATGGIGAAAVKAWLAEGADVFAMSRTSKHDTTNDL
                     KSRLISIPCDIRSEKSVEEAVSKISEHTPTLDVLVHCAGVGFFDTIENTSHHDWTQVI
                     DTNLNGSFYLTKALLPLMRKSSKAHIFLVGSTAGRKGFNKNGAYSASKFGLFGFCEVL
                     REEMRPLGIRVTHLTVGGVRTDFWEKNKTTFDTQTMIPPADIANTVVYAYHVPPPTVI
                     EEMIIKPSHGDY"
     gene            1239..2249
                     /locus_tag="PK754_14995"
     CDS             1239..2249
                     /locus_tag="PK754_14995"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNB10858.1"
                     /translation="MMELQTKDTIKEDEAQRNAEDNKRPTRVLVVDDDDDGLYILKLL
                     LSKMKYEVYTAKDGEEALHKAEALIPDIILLDVMMPKLNGFEVCKRVKATEEGQYIPI
                     ILLTAKSELMSKIEGLDCGADEYLTKPYDMGELQARIRSMLRIKQLNDSLRNANRQLE
                     ELSVTDELTKLYNRRYINKKLDDEFRRAVRYKRPLSVIMFDADHFKSVNDTYGHAFGD
                     VVLKDIAAIILETARLVDICGRFGGEEFIAILPDTDIENAFVVADRIRKKVEEHEFKD
                     ATNSIRRTVSVGISSLPDDLMNDQFTLIEWADKGLYYAKEHGRNQTVRYSDLPEYKQI
                     KG"
     gene            2246..2518
                     /locus_tag="PK754_15000"
     CDS             2246..2518
                     /locus_tag="PK754_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNB10859.1"
                     /translation="MNRAVITVSGIVQGVGYRRFVEKLAAECKLNGWVKNLENGDVYC
                     VVEGHDTELNQFLQYLRKGPTFARVTNVNVEWVHATSEFTSFMIKY"
     gene            2539..>2860
                     /locus_tag="PK754_15005"
     CDS             2539..>2860
                     /locus_tag="PK754_15005"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNB10860.1"
                     /translation="MDYKKYIREVPDFPKPGINFKDISTLVGNADAFRHVIRTMTEDA
                     AELKVDLVAGIESRGFIFGAAIAEKMGIGFIPVRKPGKLPYQTISASYALEYGENTLH
                     MHTDA"
BASE COUNT          842 a          579 c          694 g          745 t
ORIGIN      
        1 gatcggattg atgcctttga tcgttgcggt gacttttatg atgtggcgtg atacatccgc
       61 gctcaaaata cgccggctga atcaacttct gaagttagcc atgtttatcg gattatttgc
      121 aatatacaac ggataatgag gacaatgaat atacagtatg atgaaataga aatttcaaaa
      181 ccttttgtga atgaaccggg gctggatgac gaccggactt tgattttgca aacgaataat
      241 gcacgccgat ggctacataa gctcattgac gaaatgacgc tggaacagcg atttctttta
      301 ttggattacg cccagtctct aataacccat cagccgcatg aggaaacctc atctacgctg
      361 gactcaaaaa taaatgtact gcgtgcgatc gaagaagcgg ccaaaaaaag aaatgaagag
      421 cctttggcaa atctcatcag tgacattatg cgtccacatt agaaaagggt tgctagtgcg
      481 cgcataattg cgtagtttta cttagtttat catatcaata aatcaccgta aagtatggtt
      541 ctcaaagtgg ccgtaaccgg agcgaccggc ggtattggcg cggcagcggt gaaagcatgg
      601 ttggctgagg gtgcggatgt ttttgcaatg tcccgaacct caaaacatga tacaacgaac
      661 gacttaaaaa gtcgtctgat ttcgattcct tgcgatatac gttcggaaaa atcggtcgag
      721 gaagcggtct ctaaaatatc tgaacacaca ccgacacttg atgttttggt tcactgcgca
      781 ggcgtcggat tttttgatac gatcgaaaac acatcgcatc atgattggac gcaggttata
      841 gataccaatc tgaacggttc tttttatttg acgaaggccc ttttgccgct tatgcgaaag
      901 agtagcaaag cgcatatttt tttagtcggt tcaacggccg gacgcaaagg atttaacaaa
      961 aacggcgcat acagcgcgtc caagtttgga ttattcggat tttgcgaagt gctacgcgaa
     1021 gaaatgcgac cgttaggaat tcgcgtaaca catcttacgg tcggcggtgt gcgaactgat
     1081 ttttgggaaa agaacaaaac gacgtttgat acccaaacca tgataccgcc cgcagatatt
     1141 gcaaatacgg tggtttatgc gtatcatgta ccaccaccta cggttatcga agaaatgatc
     1201 ataaaacctt cccatggaga ttattaaaag gcgaaaccat gatggaattg cagacgaaag
     1261 acacaatcaa agaagacgaa gcccaacgca atgcggaaga caataaacgc ccgacgcgtg
     1321 tattggtcgt ggatgacgat gatgacgggt tgtacatcct caagcttctt ttatccaaaa
     1381 tgaagtacga agtgtacacg gccaaggacg gcgaagaagc gctgcataag gcggaggcat
     1441 tgataccgga cattattctt ttggatgtca tgatgcccaa attgaacggt ttcgaagtat
     1501 gcaaacgcgt gaaagctaca gaggaaggtc agtatattcc gatcattctt ctgacggcga
     1561 aaagcgaact gatgagcaaa atcgaaggac ttgattgcgg tgccgatgaa tacctcacaa
     1621 aaccttacga tatgggcgaa ttgcaagcgc gtatccgttc catgttgcgc atcaagcaac
     1681 tcaatgattc gcttcgtaac gccaaccgtc aacttgaaga attatcggtt acggatgaat
     1741 tgaccaagtt atacaaccgc cgatacatca acaaaaaact cgatgacgaa tttcgtcgtg
     1801 ccgtacgtta taaacgtcct ttatccgtta tcatgtttga cgcggatcat ttcaaatcag
     1861 taaacgatac ctatggccat gcctttggcg atgtggtgct caaggacatt gccgcgataa
     1921 ttcttgaaac ggcgcgattg gtagatattt gcggacgttt tggcggcgag gaatttattg
     1981 cgattcttcc ggatacggat attgagaatg catttgtagt ggcggatcgt attcgtaaaa
     2041 aagtcgaaga gcatgaattt aaagatgcga ccaattccat tcgtaggaca gtgagcgtcg
     2101 gtatttcgtc acttcctgac gatctgatga atgaccagtt tacgttgatc gaatgggccg
     2161 ataaaggttt atactacgct aaagaacatg gtcgtaacca gacggtacgc tattcggatc
     2221 tgccggagta caagcaaatc aaaggatgaa tcgggccgtc atcactgtca gcggaatagt
     2281 gcaaggtgtc gggtatcgcc gatttgtgga aaagctcgct gctgaatgta agctgaacgg
     2341 ttgggtaaaa aacctcgaga atggcgatgt atactgcgtt gtcgaaggtc atgataccga
     2401 actcaatcaa tttctccaat atcttcgcaa aggcccgacg tttgcgcgtg taacgaatgt
     2461 aaacgttgaa tgggtgcacg caacatccga attcaccagt tttatgatta aatattagtt
     2521 ttaagtgtgc atcgtattat ggactataaa aaatatatac gtgaagttcc ggatttccca
     2581 aagccgggaa ttaattttaa agacatttcg acattggtag gtaatgcgga tgcttttagg
     2641 catgttattc gtacgatgac agaagacgcc gctgaactga aagttgatct tgttgccggc
     2701 atcgaatcgc gcggatttat ttttggcgcg gccatcgctg aaaaaatggg tattggattt
     2761 attcccgttc gtaagccggg gaaattgccc tatcaaacga tatctgcttc gtatgcactt
     2821 gagtatggtg aaaacacgtt gcacatgcat accgatgcga
//
LOCUS       DAOQNQ010000368         2859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_18901_length_2859_cov_5.077746, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000368 DAOQNQ010000000
VERSION     DAOQNQ010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18901_length_2859_cov_5.077746"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PK754_15010"
     CDS             complement(<1..284)
                     /locus_tag="PK754_15010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB10861.1"
                     /translation="MRILIVAMKFFPASGGSATYAHHLAQGLHHQGHQVRLLAPKYKR
                     KNISESSDYEIVRMPLTNEVWYGIRIIFATIAIITHYLKFRPDVIWSSTFA"
     gene            complement(535..2463)
                     /locus_tag="PK754_15015"
     CDS             complement(535..2463)
                     /locus_tag="PK754_15015"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNB10862.1"
                     /translation="MNERDVVATLRFLSFFWLVFLCFFTLARAAFIYEFDEGQMNSYA
                     DIGQLFWIGGKNDIINIAYLTGIPTLLLLVRMFWGVSLTRTERFFRICVYVTVCYISA
                     VVIADWGYYGYFGSRLNASLFNLMSSSDVVFSMLYSQYALLVVIWILLSYLFVRLMQF
                     GFNRYSVVIASKPRLNRASILIALMIVGLWFQASRGFAWHRKFREGSATAFFSNNVFL
                     NTAAINPVNGFTKDAWRMAFRGSKKINWMSEQEAFTVLHRYYPESGSSSTRLLAHEIR
                     GIKPASDQNIVIVLMESMASKFVGALGNKENLTPHLDSIAAQGLLFNNFYSSGIHTYN
                     GVHSTLFGLPAIFKQHPMNTTQALQEHAGLSHMLKERGYRTVFFSPHQATFDNIGGFL
                     GMNAFDRVYDPSDYRDAETANLWGVDDRSLFQSAGKVLDTLADHSPFFSVILTVSNHG
                     PYAIPDDFSPYPGEKDRSHAVRFADQSIGSFMASVKKSKWFDRTIFVFLGDHGARGVN
                     TYDMDLEYLHIPFIIWSKGLPPSIISDLGGQIDIPATIMGLIGGDYTNNTFGIDLLRE
                     RREFLPINSDTKVGCLSKSFLLSLTPKNKPVVSRLSMDIAVPDSQVVVARMSEYLKSI
                     MQSAQWLIDHRQLSLSKK"
BASE COUNT          933 a          630 c          489 g          807 t
ORIGIN      
        1 gcaaacgtcg agctccagat cacatccggg cgaaatttta aataatgtgt tataatagct
       61 atcgtcgcaa aaataattcg tattccgtac cacacttcat tggttaacgg catacgcacg
      121 atttcataat cagaactttc agatatgttt tttcgtttat atttcggagc caacaaacga
      181 acttgatgac cttgatgatg aagcccttgt gcaagatgat gcgcataggt agcactgccc
      241 ccgctggcag gaaaaaattt catcgctacg atgagaattc gcatacgact ccaattaggt
      301 tataattagt agttattaaa aatacaagct tgatagagag agaataaaac ctaaaaatta
      361 ggcatccatt aaattaaatt ttcatgagca acgttaaacc attaacctat tccgacagtt
      421 ataaaacccc tgcaaagcaa acaacacaaa tatctagtgg gttcttaaaa ttttttacac
      481 ttattagcaa ctcagccggg ttttagtcac gccaatggcg aagcactatc ctaattattt
      541 tttacttaga gacaattggc ggtgatcaat aagccactgc gctgattgca taattgattt
      601 caaatattcg gacatccttg caactacgac ttgcgaatct ggaactgcta tatccattga
      661 caatcgtgat acgacaggtt tattttttgg agtaagcgac aataaaaagg atttggacaa
      721 acaccctact ttggtgtctg aattaatagg caaaaactcc ctccgttcac gcagtaagtc
      781 aattccaaat gtgttatttg tataatctcc gccgattagc cccatgatcg ttgccggtat
      841 atctatttgt ccgccaagat cacttataat actaggtgga aggcccttac tccaaataat
      901 aaatggaatg tgaaggtatt cgagatccat atcatacgtg tttacgccgc gcgccccgtg
      961 atcccctaaa aaaacaaaga tcgtacgatc aaaccacttg gattttttta ctgacgccat
     1021 aaatgaaccg atactttgat cggcaaaacg taccgcatgc gatctatcct tttcgccggg
     1081 ataaggtgaa aaatcatccg gtattgcata gggtccatga ttgctcaccg ttagtatgac
     1141 agagaagaaa ggcgaatgat cagccaatgt atccaatact ttgccggcag actgaaaaag
     1201 actacgatca tccacacccc acaggttagc cgtctccgca tcacgataat cggacggatc
     1261 ataaacacga tcgaacgcat tcatccccaa aaaaccaccg atattatcaa acgtagcttg
     1321 atgcggtgag aaaaagaccg tacgataacc tcgctctttt agcatgtgac taagtcctgc
     1381 atgttcttgt aaggcttgag tcgtattcat cggatgctgt ttgaatatgg ccggcaaccc
     1441 aaaaagtgta gaatgcacac cattataagt atgtattcca gaagagtaaa aattattaaa
     1501 caacaacccc tgcgcagcga ttgaatccaa atgcggggtc aaattctctt tattccccaa
     1561 cgcacctaca aattttgacg ccattgattc cattaggaca attacgatgt tttgatctga
     1621 tgccggcttg ataccgcgta tttcgtgcgc aagcaaacgg gtcgaactgc ttccggattc
     1681 cggataatac cgatgtagca cggtaaaagc ctcttgttca gacatccaat taattttttt
     1741 ggatccacga aatgccatcc gccaagcatc cttggtaaaa ccgttaaccg ggttgatcgc
     1801 cgcagtatta agaaaaacat tgtttgaaaa aaaagcggtg gcggaccctt ctctaaactt
     1861 tcgatgccat gcaaaaccac gtgacgcctg aaaccacaaa ccaacgatca tcagcgcaat
     1921 caaaatcgaa gctcgattta atctcggttt agaggctatt acgactgagt atctattaaa
     1981 cccgaactgc attaagcgca cgaaaagata actgagtaaa atccatatga cgacaagcaa
     2041 tgcgtactgt gaatacaaca tcgaaaaaac aacatccgaa gacgacatta ggttgaataa
     2101 tgatgcattt aggcgtgaac caaaataacc atagtacccc caatcggcaa tgacgactgc
     2161 actgatatag catactgtca catatacaca tatcctgaaa aatctttccg tacgagttaa
     2221 cgatacgccc caaaacatgc gaaccaataa tagaagcgtt ggaatccctg tcagataagc
     2281 tatattgatt atgtcgtttt ttccgccaat ccaaaataat tgcccaatat ctgcgtagct
     2341 attcatttgg ccttcatcaa attcatatat aaatgcagcg cgagcaagcg taaaaaaaca
     2401 taaaaaaacc agccaaaaaa aacttaaaaa ccgaagcgtt gctaccacat ccctctcatt
     2461 catagagctt tcttccctta tttaaaaacc tatattatat gatttactac gaatgtatat
     2521 tatttattat tagtattgtt cagacattgt gagtataatt tttttaatta aaaagaaaaa
     2581 caagttttca aaggcaaata ttataccaac aagcaaaact atgtttttta aattcagcgc
     2641 ttttcatttt aattcaaact tatgatctca tttttttatg cttatgatta tactgatttt
     2701 cgctccttga tttttacttc atctgaaaaa ccgtcactca gaattcttcg agtccgtctc
     2761 gttacgttta ttaaaaattt tcacactgcc atattttaat tgcacacacc atgattaatt
     2821 tttttcgttc tatcttctcc tcaaaaccca agcctccgg
//
LOCUS       DAOQNQ010000369         2854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_18971_length_2854_cov_4.656306, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000369 DAOQNQ010000000
VERSION     DAOQNQ010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18971_length_2854_cov_4.656306"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PK754_15020"
     CDS             <1..359
                     /locus_tag="PK754_15020"
                     /inference="COORDINATES: protein motif:HMM:NF019448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10863.1"
                     /translation="EAECKTSVYAQTDGFVTTTDTLQIGLASVAIGAGRSKKEDTIDP
                     TAGIVIKKKVGDAVQANDLIAEIYHSPRVDGKAVAHRIQSAYRIEYNAPDKKPLIYGI
                     LDKNGLKEIENNRNLE"
     gene            389..1168
                     /locus_tag="PK754_15025"
     CDS             389..1168
                     /locus_tag="PK754_15025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB10864.1"
                     /translation="MAKRAIKKNKPTAKAKRKPVQRSLKTPLQSVVSEQAPSKPKLDD
                     DLDIDVPITPEDPNRLLLEKREKQKKEDFEFIARAVGGDQKAFELLMNRYRPQIFGLM
                     YRMVHKSEEAEDLVQEAFMKAFTSLSNFNYEYAFSTWLYKIATNNCIDHLRKKKLQTY
                     SIDAPVQYKDSEYQIEIPDLTYHPEKEMMRNERTQMIKGAIEELPEKYKRVIFLRHSE
                     ELSYEEISEMLNIPIGTVKARIFRAREMLNKFLKGKIDKVL"
     gene            1204..2025
                     /locus_tag="PK754_15030"
     CDS             1204..2025
                     /locus_tag="PK754_15030"
                     /inference="COORDINATES: protein motif:HMM:NF028425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HNB10865.1"
                     /translation="MWLFIFQVLPAVGQYDVQEIERTDEKELKVRLEYAAGKLSLKKI
                     STDKLARITIQSEDREVKPTVDYEKNGTTGMISMRMDEENNVGFFDVGNQDIQMYLTD
                     KIPVSFKIDLGACESEMDFTQLRVRDLTLNMGASSSNIRFSSLNRDRINKFRIEAGIS
                     KLNMYGLGNANFEKLEFEGGVGNYMLDFSGTMTQSASAHITVGIGSLIIRLPQNLAVK
                     IHTDESYLSSIRIDQNDFVKKRDGLYISKGFDAADAVLELHLETGLGKLRVESIK"
     gene            2037..>2854
                     /locus_tag="PK754_15035"
     CDS             2037..>2854
                     /locus_tag="PK754_15035"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB10866.1"
                     /translation="MFSRVLLTATFYVAVFLFKTVPISAQSGDPLNLAQSLEADGQFE
                     KAVTIYEKLYQKDPSNIVVADRLKRAYKTLGKYELRERVLLAQIQNDSSQIAFWAELA
                     DNYYKLKRPERAATIIQKYISTEQSQIGNYRVFASILMENRRWDELERVYKSARKTLR
                     NEKLFIAEMANLQQYRGDYHAATLEFLSFWSYGMGTFDYVRQQILQFGDVSADLDTIV
                     NAVKIYIQQLPKEPKLLKLIGELQFKTGRMDAAFATTIQLDEQFQKQGTEILAFA"
BASE COUNT          913 a          533 c          645 g          763 t
ORIGIN      
        1 ctgaagccga atgcaaaact tcggtgtatg cccaaacgga cgggtttgtc acgactacgg
       61 atacattgca gatcggtcta gcgtcggttg cgatcggtgc cggacgcagt aaaaaagaag
      121 atacgattga tccgacagcc ggtatagtta taaagaaaaa agtaggtgat gccgtccaag
      181 ccaatgatct cattgccgaa atttatcatt caccccgcgt ggatggaaaa gcggtcgcgc
      241 atcgaataca gagtgcttat agaatagagt ataatgctcc ggataaaaaa cccttaattt
      301 acggcattct ggataagaac ggtttaaaag aaatcgaaaa taatcgaaac ttagaataaa
      361 catttaacgt atcactattg gtttggaaat ggccaagcgc gcaataaaga aaaataaacc
      421 gactgcgaaa gccaagcgta agccggttca acgctctttg aaaaccccgc tgcaatcagt
      481 ggtttctgaa caagcaccgt ccaagccgaa acttgacgat gatttggata ttgatgtgcc
      541 gataacgccg gaggatccca atcggcttct tttggaaaag cgtgaaaagc agaagaaaga
      601 ggattttgag tttatcgctc gcgctgtcgg tggtgatcaa aaagcattcg aactactgat
      661 gaataggtat cgtcctcaga tattcggctt gatgtatcgt atggtgcata aatcagaaga
      721 agccgaggac ttggtacagg aagcatttat gaaggctttt acatcattaa gtaattttaa
      781 ttatgaatac gcattcagta cttggttata caaaattgca accaataact gtatagacca
      841 ccttcgcaag aaaaagttgc agacgtattc gattgatgca ccggtacaat ataaggattc
      901 tgagtatcag attgagatac ctgatcttac gtatcaccct gaaaaagaaa tgatgcgtaa
      961 cgagagaacg caaatgatca agggcgcgat cgaagaatta ccggaaaaat acaaacgggt
     1021 tatattttta aggcattcgg aagaattatc gtatgaagaa atttctgaaa tgctcaacat
     1081 accgatcggc acagtaaaag cgcggatttt tcgcgcacgt gaaatgttga ataaattcct
     1141 taaaggaaaa atagataagg tattgtaatg acgttcaatc cagcaaataa aagcttctac
     1201 tttttgtggt tgtttatttt tcaagtgttg cctgccgttg gacaatacga tgtgcaggag
     1261 atcgaacgta cggatgaaaa ggaattaaaa gttcgtctcg agtatgccgc cggcaagtta
     1321 tctcttaaaa agatttcaac ggacaagctt gctcgaataa cgatacaatc tgaagatcgt
     1381 gaagtcaaac cgaccgtgga ctatgaaaaa aacggtacga ccggtatgat ttctatgcgt
     1441 atggacgaag aaaacaatgt gggcttcttc gatgtgggca accaagatat acagatgtat
     1501 ctgacggaca agatacctgt atcgtttaaa atagatttgg gtgcctgtga gagtgagatg
     1561 gattttactc agctacgcgt tcgcgatctt acgctgaata tgggcgccag ttcgtccaat
     1621 atacgcttct cgtcactcaa tcgtgatcgc atcaataaat ttagaattga agccggtatt
     1681 tctaagctta atatgtatgg cttaggcaat gccaacttcg aaaaactaga gttcgaaggc
     1741 ggtgtaggaa attatatgtt ggatttttcg ggtacgatga cacaaagtgc ttcggcgcat
     1801 attactgtag gcatcggtag tcttattata cgattgcctc agaaccttgc cgtaaaaata
     1861 catacggacg aatcgtattt atcatcaatt cgaattgatc aaaatgattt tgtgaagaag
     1921 cgtgacgggt tatatatttc caaaggattt gacgctgcgg atgctgtttt ggaattacac
     1981 ttggaaaccg ggctaggaaa acttcgcgtg gaatccatta aataatcagg gatgatatgt
     2041 tcagtcgtgt tttattaaca gcaacatttt acgttgctgt ttttcttttt aagacggttc
     2101 caatcagcgc gcaaagcggt gacccgctga acctcgcaca aagtctggaa gcggatggtc
     2161 aatttgaaaa agcggtgact atttatgaaa aattatatca aaaagacccc tcaaatattg
     2221 ttgtagctga tcgcctgaaa cgcgcgtata aaactttagg aaaatatgaa ttgagagagc
     2281 gcgtactgtt ggcacagatt caaaatgatt cgtcccaaat tgcattttgg gctgaattag
     2341 ctgataatta ttacaaactt aaacgcccgg agcgggctgc tacaataata caaaagtata
     2401 tttctactga gcaaagccaa atcggaaatt accgcgtatt tgcttcgatt ttaatggaaa
     2461 accgccgatg ggatgagttg gagcgagtgt ataaatcggc acgtaaaaca ttgcgtaacg
     2521 aaaaactgtt tattgctgaa atggccaact tgcagcaata tcgcggtgat tatcatgccg
     2581 ctacgctcga attcctatca ttttggtctt acggcatggg gacatttgat tacgtgcgtc
     2641 aacaaatatt acaattcggc gatgtatccg cagatttgga tactatcgta aacgctgtaa
     2701 aaatctatat tcagcaatta cccaaagaac ctaaactgct caagctcatt ggagaacttc
     2761 aatttaaaac cggacgcatg gatgcggcgt ttgctaccac tattcaatta gacgagcagt
     2821 ttcaaaaaca ggggacggag atcctcgctt ttgc
//
LOCUS       DAOQNQ010000370         2850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_19032_length_2850_cov_4.786762, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000370 DAOQNQ010000000
VERSION     DAOQNQ010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19032_length_2850_cov_4.786762"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PK754_15040"
     CDS             <1..403
                     /locus_tag="PK754_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10867.1"
                     /translation="DLRRTHQDLRVMQSDMRMQDGAYAREADIDRVISDLTTFKLNVT
                     YLRLKAGELVQRNNLEFSEVREEVRYFATYLKHASDTFDEICNSMAYGGRSGDDAAMQ
                     KTGEELLILQRHLQEMNEHLLVINSKLGKTS"
     gene            424..819
                     /locus_tag="PK754_15045"
     CDS             424..819
                     /locus_tag="PK754_15045"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNB10868.1"
                     /translation="MKQSKKVQIASESAVKVTTHRITDDLASVEIDDKMMTEEHMKML
                     EKAVHELLAENYHSIILDLTKLKRINSTGLGKFINLYKAAADKDGVMKIGGVSDFVRN
                     VLTSTKLNEIFEIHNTLDDAIKSFQTSGA"
     gene            979..1054
                     /locus_tag="PK754_15050"
     tRNA            979..1054
                     /locus_tag="PK754_15050"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1012..1014,aa:Ile,seq:cat)
     gene            1211..1786
                     /locus_tag="PK754_15055"
     CDS             1211..1786
                     /locus_tag="PK754_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10869.1"
                     /translation="MRFSQIVSLFLIVSFVSFTACDSDSKSTVDASEQKPVLSLTQHD
                     STILTQAYVNSSWYEFGPRLRFLKNGTITGLGMMMPDSGSYEVTLWDLSDTSIVTTVT
                     IAADSGELKYVAIDPIQVQTDERYSVTILSDDWYWFEDISGKNNFLPQTFDDVMIEDY
                     AYISGSTHQFPTDAFTDYLAGIQDIIFVPEK"
     gene            complement(1941..2582)
                     /locus_tag="PK754_15060"
     CDS             complement(1941..2582)
                     /locus_tag="PK754_15060"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNB10870.1"
                     /translation="MQYQSRNNRYTLMMDISVIITIVLAYIILHTVTITEYAKAEIRT
                     PTQIELTRVDLTASHHAPPPRRPVVPVATDQETLLGDETIDDTDIDLDETPPPPPPPF
                     KKVEKGDDDVFTVYDTRPLLKGGADFLKRNLKYPEAAQQAGIEGVALVRAVVDEKGNV
                     VKAEVVKEDGNVGFGQAAIDFVLKCKFEPATFKNKPVKVAVTLPITFSKKNLL"
     gene            complement(2586..>2850)
                     /locus_tag="PK754_15065"
     CDS             complement(2586..>2850)
                     /locus_tag="PK754_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10871.1"
                     /translation="LSTKTHTSYLWIDKQQRIVFDDYPINSMEELYSVARAKIEADHK
                     MLIFLRVDESLTMGALADVQEQLRRAGALRIYYGTDSRSNAIY"
BASE COUNT          806 a          629 c          587 g          828 t
ORIGIN      
        1 agatttgcgt cgtacgcatc aagatttgcg cgttatgcag tcggatatgc gtatgcagga
       61 tggcgcgtac gcgcgcgaag cagacattga ccgtgtcatc tccgacctca ccacattcaa
      121 gttgaatgtg acatatctgc gccttaaagc cggcgaactc gttcagcgga ataatcttga
      181 gttctcagaa gtgcgcgaag aagtgcgtta ctttgcaacc tacctcaaac atgcctccga
      241 cacatttgat gaaatctgta acagtatggc ctacggaggt cgttccggcg atgatgccgc
      301 catgcaaaaa accggcgaag aattactcat cctgcaacgc caccttcagg aaatgaacga
      361 gcacctttta gtcattaatt cgaaattagg aaagacttca taacttttat gtacgaaaaa
      421 cccatgaaac agagcaaaaa agtacaaatc gcaagcgaaa gcgccgtcaa agtgaccacc
      481 catcgtatta cggacgactt agccagcgtg gaaattgacg acaagatgat gacggaagag
      541 cacatgaaaa tgctcgaaaa ggccgtccat gaattgcttg ccgaaaatta tcactcgatc
      601 atattggatc ttaccaaact caaacgtatc aacagtaccg ggcttggtaa atttattaac
      661 ctctacaaag ccgcagcgga taaagacggc gtcatgaaaa tcggtggtgt ttctgacttt
      721 gtacgcaatg tacttacgag taccaaactt aacgagattt tcgaaattca taatacactg
      781 gacgatgcca tcaaaagttt tcagacatcc ggcgcataag acgctcttaa attattattc
      841 caaaaaaacg cggggtttat actccgcgtt ttttatttga aatattttca gaaaaacctt
      901 tgctatcgtt tcaaaaaata ttatttgaat ttgtatcttc attttatatc tttgcaccgc
      961 tttttatata tgttcaaagg gccgttagct cagcggttag agcaggggac tcataatccc
     1021 ttggtccggg gttcaaatcc ctgacggccc accataaaac cttaagttat aaacttaggg
     1081 ttttattatt tgtgcaaccg gtttaaccac tcccttccca tatatattac aaaaccgcat
     1141 taacatgtag gccattttta ttttgtatta ttctattatt ttataattat caaatcaaat
     1201 cggagaaaat atgcgtttta gccaaattgt atcgttgttt ttaattgtta gttttgtaag
     1261 ttttaccgca tgcgactctg atagtaagtc aacggtagac gcttccgaac aaaaacccgt
     1321 tctttcgctc acccaacatg attccacgat tttaactcag gcttatgtca acagctcatg
     1381 gtatgaattt ggtccgcgcc ttcgtttttt aaaaaacggg acgatcacag ggcttggtat
     1441 gatgatgccc gattccggtt catacgaagt tacgctttgg gatttatcgg atacgagcat
     1501 tgtcactaca gtgacgatcg ctgcggactc cggagagcta aaatacgttg caattgatcc
     1561 tatccaagtg cagaccgacg agcgttattc ggttaccatt ttatccgatg attggtattg
     1621 gtttgaagac atctcaggca aaaataattt tttacctcag acattcgatg acgtaatgat
     1681 tgaagattac gcttacatat ccggctcgac gcaccagttc cctactgacg cgtttaccga
     1741 ttatttggct gggattcagg atattatttt tgttccggag aaatagtcaa aatttattta
     1801 cctcccggcg ttctacagtt tacagatcag attcaaattc gatgaaaata aataggcatg
     1861 aataagaaaa ccccttaagc cgatcggact taaggggttt attaataaaa tttagatgct
     1921 actaaataac agcgattacc ttataaaaga ttctttttgc taaacgtgat cggcagtgtt
     1981 acggccactt tcaccggttt gtttttaaac gtagccggtt caaatttgca ctttagcaca
     2041 aaatctatgg ccgcttgacc gaaaccaaca ttaccgtctt cctttactac ttcggctttg
     2101 accacgtttc ctttttcatc cacgacagca cgaactaacg ccaccccttc gatacccgct
     2161 tgttgcgcgg cttccgggta cttcaggtta cgtttaagaa aatcagctcc gcctttgaga
     2221 agcgggcgtg tatcgtaaac tgtaaatacg tcgtcatcgc ctttttcaac ttttttaaac
     2281 ggtggtggtg gtggcggtgg tgtttcatcc agatcaatat ccgtgtcatc tatggtctca
     2341 tcgccgagga gcgtttcctg gtctgtagca accggcacaa ccgggcggcg cggtggtggt
     2401 gcatgatgcg aagccgtaag atccacgcgt gttaactcaa tctgtgtcgg cgtacgtatc
     2461 tcagcttttg catattcggt aatcgtcacg gtatgtaaaa taatatatgc aagaacgatg
     2521 gttatgatga cagaaatatc catcatcaac gtataacgat tatttcgtga ttgatactgc
     2581 atagcttaat aaattgcgtt agaacgtgaa tcggtcccgt agtaaatccg gagcgctccg
     2641 gcacgacgca actgttcttg cacgtccgcc aaagcgccca tcgtcaacga ttcgtccaca
     2701 cgtagaaaga taagcatttt gtgatcggct tcgattttcg cgcgggctac cgaatataac
     2761 tcttccatcg aattaatcgg ataatcatca aatacaatac gctgttgttt gtcaatccaa
     2821 aggtaagatg tatgtgtttt cgtggataat
//
LOCUS       DAOQNQ010000371         2839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_19164_length_2839_cov_5.604526, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000371 DAOQNQ010000000
VERSION     DAOQNQ010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19164_length_2839_cov_5.604526"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PK754_15070"
     CDS             <1..470
                     /locus_tag="PK754_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HNB10872.1"
                     /translation="YVDPSDIRLFDYTFLYMAGHGNVKFSDDDVRQLRRFLTGGGFLW
                     CDDDYGIDPYFRREMKRVFPELDFTLIPFSHPIYSIGSRFPNGMPKIHEHDGGPPQGY
                     GLFWKGRLICFYSKNTDISDGLEGPEVFPEDHPEKQAEALRFAANIVMYALSY"
     gene            484..1179
                     /gene="ccsA"
                     /locus_tag="PK754_15075"
     CDS             484..1179
                     /gene="ccsA"
                     /locus_tag="PK754_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNB10873.1"
                     /translation="MKNGVGEKILDAAMLVVMLAALYLTFIWVPNEKTMGIIQRVFYF
                     HVPAAWVSFLAFGFVFVASIMYLIKRDDKWDAMAVCSAEIGLLFCTIVLVTGPIWAKP
                     VWGIWWTWDARLTLTLVLWLIYLAYTMLRAYIDDPLKRGRLSAVVGIVGFVDVPLVYF
                     SIRWWRTQHPAPVMAGGEGSGLAPEMRMVLMFCLAAFTLLFVYLLNKRLKMEKTEREL
                     ELLYRTVQRDNRM"
     gene            1254..1547
                     /locus_tag="PK754_15080"
     CDS             1254..1547
                     /locus_tag="PK754_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10874.1"
                     /translation="MLLLRTTVFAGDGYQFLRVGVTARATGLGDVFVAQPGDATTFMY
                     NPAGLATLQGRTFAASYKESLPLSLQVGVNYKLQNAPVTVNLTFTDIQTTVSV"
     gene            1660..2517
                     /locus_tag="PK754_15085"
     CDS             1660..2517
                     /locus_tag="PK754_15085"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB10875.1"
                     /translation="MKEEQTLVNDVKQFWSSHPMAYNFPTKNLSKDLLAQIDQKFYKK
                     AVNCYHGDGKKLYSDYINYNEMIKNKSVLEIGYGMGTMMQCFCENGANYFGIDLSDFH
                     FAAAQFRKSMFNLNADVVLGNAESLPYGDNQFDFVFSHGVIHHTPQTEKVCDEIYRVL
                     KPGGKFLVMVYHLNSIRVRWDYHIKHSFFRWQKGLKTFCSIQEALNYHTDNHDSNRGG
                     SPLAKAYTRTTGAKLFEKFKHLHIDVTSNPIEFEKFPFHPFGKLMPFSLRKKLSKKWG
                     YFLMINGQK"
BASE COUNT          773 a          514 c          679 g          873 t
ORIGIN      
        1 cctacgtgga tccgtcggat atccggcttt ttgattatac ctttttgtat atggccggtc
       61 acggcaacgt caaattcagc gatgatgatg ttcgccaatt gcgtcgtttt ctgaccggcg
      121 gcggttttct ttggtgcgat gatgattatg gtattgatcc gtattttcgg cgtgagatga
      181 aacgtgtgtt tccggaactt gattttacgc tgattccttt ttcacatccg atttattcga
      241 tcggatcacg gttccccaat ggtatgccga aaatacacga acacgacgga ggccctccgc
      301 aaggttacgg attgttttgg aaagggcgat taatttgttt ttattctaaa aatacggata
      361 tcagcgacgg cctcgaaggg ccggaagtat ttcccgaaga ccatcctgaa aaacaagcgg
      421 aggcacttcg gtttgcagcg aatattgtaa tgtatgcctt aagttactga gaaggtgatg
      481 aatatgaaaa atggtgtcgg tgaaaaaatt cttgatgcgg ctatgttggt ggttatgctg
      541 gcggcgctgt atctgacgtt catttgggtt cccaatgaaa agaccatggg tatcattcaa
      601 cgcgtgtttt attttcatgt gcccgccgcg tgggtttcat ttttagcgtt tggttttgtt
      661 tttgtcgcga gcatcatgta tctgatcaag cgggatgaca aatgggacgc catggccgta
      721 tgttcggcgg aaatcggttt gcttttttgt acgatcgtgt tggtgaccgg gccgatttgg
      781 gctaaaccgg tgtggggcat ttggtggacg tgggatgcgc gcctgacatt gacgttggtt
      841 ctgtggctta tatacctcgc atacacgatg ttacgggcat acattgacga tccgctcaag
      901 cggggacgcc tttcagccgt cgtcggcatc gtgggatttg tagatgttcc gctggtttat
      961 ttttcgattc gctggtggcg tacacagcat ccggcacccg tcatggcggg cggcgaaggt
     1021 tcgggtttgg caccggaaat gcgtatggtg ttgatgtttt gccttgccgc atttactctg
     1081 ctttttgtgt atctattgaa taaacgcctg aaaatggaga aaaccgaacg cgaacttgag
     1141 ttactttatc gtacggtgca acgggataac cggatgtagt tttttgcgat agcatagttg
     1201 gcgccaagac gttatattaa aaaacgatga gaaaaataat tctaatagtt tgtttgttgc
     1261 tgctccggac gaccgtgttt gccggggacg ggtatcaatt tttgcgtgtc ggcgtgacag
     1321 cccgtgcgac gggactcggc gatgtgtttg tcgctcaacc cggtgatgca acaacgttta
     1381 tgtacaatcc ggcaggcctt gcaacactac aagggcggac ttttgcagcc agctataaag
     1441 aaagcctgcc gttatcattg caagtcggcg taaattataa actgcagaat gccccggtta
     1501 cggtcaatct gacatttacg gatatacaaa cgactgtcag cgtatgaatt gagcgctatg
     1561 gctgcacaat tagcaaagac gaaattttca gttgattttt tttgactcaa aataaaaaaa
     1621 ttcaaactac tttttttaaa acaaaaaaag gatagatcca tgaaggaaga acagaccttg
     1681 gttaatgacg taaagcaatt ttggtcaagt catccaatgg catacaattt tccgactaaa
     1741 aacctttcga aagatttgct tgcgcagatt gatcaaaaat tttataaaaa ggctgtcaat
     1801 tgttatcatg gtgatggcaa aaagctttac tccgattata ttaactataa tgagatgatc
     1861 aagaataaga gtgttctgga aattggatac ggtatgggca ctatgatgca atgtttttgt
     1921 gaaaacggag ctaattattt tggaattgat cttagtgatt ttcattttgc tgcagctcaa
     1981 tttcgaaaat ccatgtttaa tcttaacgcc gatgttgtat taggaaatgc agaaagtttg
     2041 ccttatgggg ataatcaatt tgattttgtt ttttcacatg gggttatcca tcatacgccg
     2101 cagaccgaaa aggtatgtga tgaaatttat agagtgctaa agccgggcgg taaatttctt
     2161 gttatggttt atcatttgaa ttcgattcga gtaagatggg actaccacat taaacattcc
     2221 ttttttcgat ggcaaaaagg attaaaaaca ttttgtagca ttcaagaagc gttgaattat
     2281 catacggata accatgattc aaatcgaggc ggctcaccac tggctaaagc gtatacacga
     2341 acgacaggcg ccaaattgtt tgaaaaattt aaacacctgc acattgatgt tactagcaat
     2401 cccattgagt ttgaaaaatt tccctttcat ccttttggta aattgatgcc cttttcatta
     2461 agaaaaaaat tatccaaaaa atggggatat tttttgatga ttaatgggca aaaatgatac
     2521 aatttaaaaa catcaatact acaagataaa atcatgtccg gcattgtggg gatgatacat
     2581 gttgatccga atagcatgtc gaaaatggag tgggtttact ggcgtaattg tgcaagcgcg
     2641 ctatcgcatg ttccgttttc ttaatagctt tgattttatt gttaaacaat accgtgtctg
     2701 cgcagacaag cggaaaactg ttttatatcg ttttgcgatt gcgctactca aatagagcgg
     2761 agatggtgat tagtatggca ataaaaacgc attgaattat cggctgcgtt atttgaacga
     2821 aaatattaaa tattgctgt
//
LOCUS       DAOQNQ010000372         2834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_19237_length_2834_cov_3.656711, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000372 DAOQNQ010000000
VERSION     DAOQNQ010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19237_length_2834_cov_3.656711"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..521)
                     /locus_tag="PK754_15090"
     CDS             complement(102..521)
                     /locus_tag="PK754_15090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB10876.1"
                     /translation="MRILIIDDSELIRERLKTLITEECRDVFIETEARAAQALESVST
                     IRPDVVILDIKMRDGSGVGVLTQIKNLSSPPIVIMFTQHPYPQFKSRCIFYGADYFLD
                     KSTDMDQIPAIVVNLGKTQRLNGRISDSPGGFRHTQI"
     gene            complement(830..1195)
                     /locus_tag="PK754_15095"
     CDS             complement(830..1195)
                     /locus_tag="PK754_15095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB10877.1"
                     /translation="MRILIADDSALVYERLSSLIHELHKNLELVGHAETAPDTIRQAI
                     MQKPDVVILDIKMPGGNGIEVLKQLKSEPKPPVIIMFTQYPYPQFRDRCLQYGADYFF
                     DKTSEFDRIGDVLEKIAMN"
     gene            complement(1561..2205)
                     /locus_tag="PK754_15100"
     CDS             complement(1561..2205)
                     /locus_tag="PK754_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB10878.1"
                     /translation="MDHSASIERILIADDHAIVRKGLAETVLERYPRCHITHASNADE
                     LLGCIQAEIFDVALMDISMPGVTGLEALKQVRMIRPQLPVLILTAYPESQYAERAIRL
                     GAMGYINKSVASDELVEAIRTVKAGKKFITKAVAMILAEKDTGADEPHTNLSDREYEV
                     MRLIAQGLSPKEIGEALSISPKTVNSMRDRILEKMNLTSNAEIIRYAIQKKLVE"
     gene            complement(2214..>2834)
                     /locus_tag="PK754_15105"
     CDS             complement(2214..>2834)
                     /locus_tag="PK754_15105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNB10879.1"
                     /translation="VREEERTRISREVHDELGQQLTALKMDLSWIQKRLPEDSSLKEK
                     SNTMSSLIDATVKSVQRIASELRPGLLDDLGVSAAIEWQSSEFEKRTGIQCRCYLEQG
                     DTAVTNEQGTALFRIFQETLTNVMRHAEATEVVIEFFAEHEHLHLIIRDNGKGITKVQ
                     LNSPHSLGLMGMRERIQHLQGEITISGEQGKGTVVHVSLPIINPIS"
BASE COUNT          819 a          581 c          608 g          826 t
ORIGIN      
        1 tgaaatgtat tggatactat ctaaatacat caaaccctca tgccgaccaa acatgagggt
       61 ttgttattaa gaaaagaagg aggattcaaa acgggtacag cctaaatctg tgtatgccga
      121 aagccgccgg gcgaatcact gatacgacca ttcaagcgtt gagtttttcc caggttcaca
      181 acgattgcag ggatttgatc catgtccgta gatttatcaa gaaaataatc ggcaccataa
      241 aatatgcatc ggcttttaaa ttgaggatac ggatgttgcg taaacatgat aacaatcggg
      301 ggtgaagaca ggtttttgat ctgagtcaaa acaccgacgc cgctgccatc ccgcattttg
      361 atgtccagta tgacaacgtc aggacgtatt gtgcttacgg attctaacgc ttgtgcggca
      421 cgggcttcag tttctataaa aacatcccgg cattcctccg taatcaacgt ttttaaacgc
      481 tcgcgtataa gctccgaatc atctatgatc agtatgcgca tatacttatt ccgtcaaaaa
      541 taaaaattta gtgctcgaac gatcgtgcaa gaaaatatat aaaaccgagc agaatgaata
      601 taatacatca cgtgatcata ctagaacaca atgtctgatt agatattgat tcctacataa
      661 gataatcaaa attaagttat ttgtctgtag gtaaatggct gaaaatcgtg taggtaattt
      721 acctacaaat cttgatgagg caggattaag tttaaattaa tcaaagcaag gtaaaacaaa
      781 acaacccgcg atgagttcgg aggttgttta tgttaaaatc aaaatgggat caattcatcg
      841 cgattttttc gaggacatca ccgatacgat caaattcgct ggttttgtca aaaaaatagt
      901 cggctccata ttggaggcaa cgatccctaa actgtggata agggtactgt gtaaacatga
      961 taataaccgg cggttttggc tcggatttta gttgtttaag gacttcgatg ccgttccctc
     1021 caggcatctt aatgtccaaa attactacat ccggtttttg catgatagct tgtcgaattg
     1081 tatcaggcgc cgtctccgca tgaccgacta attccaaatt tttatgcaac tcatgtatta
     1141 acgaagaaag tcgttcatac accagcgctg aatcatctgc aatcagtatt cgcacaatgc
     1201 cccctttctc tttttgtccc ccaaaaagag acttttagtt tttacgataa tagacccaag
     1261 taacgcttga aatataacgt tcgaaatcct gaaagtatgt aggtattttt ctgaaaatct
     1321 tgtaggtaaa ttacctacaa gatttattta aactaaacat ttacaatata aacatataac
     1381 tgtttaaatt tattttaagg gttatataaa actgctttga tccaatccac tcaggtgttc
     1441 gatcattagg aaatgaaatg tgaatcaagg agaatgaaaa aaattagttg gtattcagag
     1501 gatcagcgga ggcaaacagg tattcaatac atacaaaaaa taaaagttat aaaatagaac
     1561 ttactcgact aatttttttt gaatggcgta gcggataatt tcggcattag atgttagatt
     1621 cattttttca agaatacggt ctcgcatcga attaaccgtt ttggggctga tgctgagtgc
     1681 ttcaccgatc tctttgggtg acaatccttg tgcgatcaag cgcataacct cgtattcacg
     1741 atccgataga ttggtatggg gttcatccgc tccggtatct ttttcggcta agatcatggc
     1801 tacggctttg gtaataaatt tttttccggc ctttaccgta cgaatcgctt cgaccagctc
     1861 gtcggatgcc accgatttat tgatatagcc catagcgcct aggcgaatcg cacgttcggc
     1921 gtattggctt tccggataag ccgtcagtat cagaaccggt aattgaggtc ttatcatacg
     1981 cacctgtttg agtgcttcta atcccgtgac acccggcatc gaaatgtcca tcaacgcgac
     2041 atcaaatatt tccgcctgaa tgcagccaag taattcgtcg gcattggacg cgtgtgtgat
     2101 gtggcaacgc ggataacgtt ctagtaccgt ttcggcaagg cctttgcgaa caatagcgtg
     2161 atcatcagct ataagtatgc gttcgatgga agcagagtga tccataaata attttaagaa
     2221 atgggattga taattggcag cgatacatgc actacggtac ccttgccttg ttcgccggag
     2281 atcgtgattt cgccttgtaa atgttggata cgttcacgca tgcccatgag gcccagcgaa
     2341 tgggggctat tgagttgtac tttggtaata ccttttccgt tgtctcgaat gatgagatga
     2401 agatgttcat gttcggcgaa aaactcaata acgacttccg tagcttcggc atggcgcatc
     2461 acgttcgtca gtgtttcctg aaaaatacga aacagtgccg tgccctgttc gttggtaact
     2521 gctgtgtcgc cttgttccag atagcaacga cattggatgc ccgtacgttt ttcaaattcg
     2581 gacgattgcc attcgatagc agccgaaacg ccaagatcgt ccagcaatcc aggacgtaat
     2641 tcgctggcaa ttcgctggac gcttttgacg gtggcatcga tgagtgaact catagtgtta
     2701 gatttttctt tgagggatga gtcttctggg aggcgctttt gtatccaaga aagatccatt
     2761 ttcagagcgg tcagttgttg acccagttca tcatgcactt cacgcgaaat ccgcgtacgc
     2821 tcttcttcac ggac
//
LOCUS       DAOQNQ010000373         2826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_19328_length_2826_cov_5.582100, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000373 DAOQNQ010000000
VERSION     DAOQNQ010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19328_length_2826_cov_5.582100"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="PK754_15110"
     CDS             <1..720
                     /locus_tag="PK754_15110"
                     /inference="COORDINATES: protein motif:HMM:NF020376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HNB10880.1"
                     /translation="HKLEMLLDMGVIAMLPFREILSRVLPDMLKINILAEYQLMNFDK
                     HDDDFIDFNLIWFLGKLNLQTVDKFLSDNDWHHDTPDFNNRDEESPPFGDAYDPAESE
                     SNDADASAGAFDLNEEDRLLKTELENELKHFYQDQSHFYTRVVGLRHTEFDISTLSEG
                     ARLTLVPEPENTHDPMAVSVHSARGEKIGYLKKELAALLFSELRQGVVFHATVVKVLP
                     EAEEANLRLHILVRKWTHHFN"
     gene            749..1666
                     /gene="glsA"
                     /locus_tag="PK754_15115"
     CDS             749..1666
                     /gene="glsA"
                     /locus_tag="PK754_15115"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006603184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HNB10881.1"
                     /translation="MDISTLNHAVLAAKPHLQNGRVADYIPALREADAQHLGVAVADT
                     GGAVIGAGDCQVSFSIQSVSKIFSLACVLRQRGLDAVLQHVGMEPSGNPFYSLVQLEY
                     ESGKPRNPFINAGAIAVTSLLPGQTAGEKSEFLCRFLSEISGTPIAVNEKVYFSEYET
                     SHRNRAVAHFMKHFGNLEGNVEEAVNAYFQQCSITMDAVTLARLGLFLAHHGTDPVSG
                     KTIVDAKSQRFLLAMMTMCGLYDASGEFAVRVGLPAKSGVGGGIFTVVPGRYTIVTFG
                     PALDDKGNSIGGIQILEHLSSACQLSSWV"
     gene            2217..>2826
                     /locus_tag="PK754_15120"
     CDS             2217..>2826
                     /locus_tag="PK754_15120"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNB10882.1"
                     /translation="MNIIVCMKQVPDSETRVKIGADGKSVDLSAANFVVNPYDEFAIE
                     EGIRLKEKFGGEVTILTLGTDRAENDIRKGLAMGADKATLLKADNFNGDVAHALAEEI
                     KAGAYDLVLFGKQAIDEDSSVMPQIVAEKLNLPCVTVVTKLDVNADTKAITCEREIEG
                     GKEIVELTYPAVIGTQKGLNEPRLPNLKGIMAAKKKTIDKKDP"
BASE COUNT          723 a          714 c          683 g          706 t
ORIGIN      
        1 cacaaattag aaatgttgct tgatatgggc gtcatcgcca tgttgccgtt tcgtgaaatt
       61 ttgtcgcgcg tgctcccgga tatgctcaaa atcaatatac tcgccgaata ccagctgatg
      121 aattttgaca aacacgacga cgattttatt gatttcaatc tgatttggtt tctcggaaaa
      181 ctcaatctgc agaccgtgga caagtttctc agcgataacg attggcatca cgacacgcct
      241 gattttaata accgcgacga agaatccccg ccgttcggcg atgcgtacga tcccgccgag
      301 tcggaatcca atgacgccga cgcgtcggcc ggtgcatttg atctcaatga agaagatcgt
      361 ttgctaaaaa ccgaattgga aaacgaactc aaacattttt atcaggacca atcgcatttt
      421 tatacccgcg tcgtcggact gcgccatacg gagtttgaca tcagcacgct gagcgaaggt
      481 gcgcggctga cgctcgtgcc ggaacccgaa aatacgcacg accctatggc cgtgagcgtg
      541 cacagcgccc gcggtgaaaa aataggttac ctcaaaaaag aactggcggc cttacttttt
      601 tcggaattgc gccaaggcgt agtgtttcac gccaccgtcg tcaaagtttt gcccgaagcc
      661 gaagaagcca atctccgctt gcatatactg gtccggaagt ggacgcatca tttcaactaa
      721 ccggactacg ctctcccgcg cacacaccat ggacatttca acgctcaatc acgctgtact
      781 cgcggccaaa ccgcatctcc aaaacggccg tgtcgccgac tatatcccgg ctttacgcga
      841 agcggatgcg caacatttgg gcgtagccgt agcggacacc ggcggtgcag tgatcggtgc
      901 cggcgactgc caggtttcct tttcgataca aagtgtttcc aaaatttttt cattagcctg
      961 tgtgctccgg cagcgcggtt tggatgctgt actgcagcat gtgggtatgg aacccagcgg
     1021 caatccgttt tattcgctgg tgcagcttga gtacgaatcc ggcaaaccgc gtaatccgtt
     1081 tatcaatgcc ggcgccatcg ccgtgaccag tttattaccc ggtcagacgg ccggcgaaaa
     1141 atccgaattt ttatgccgct tcctgtccga gatttccggg actccgatcg cggtcaacga
     1201 aaaagtttat ttctccgaat acgaaacaag ccatcgtaac cgcgccgtgg cgcattttat
     1261 gaaacatttt ggcaatcttg agggcaatgt cgaagaggct gttaacgctt attttcagca
     1321 gtgctcgatc acgatggatg cggttacact ggcgcgcctc gggctatttt tggcgcatca
     1381 cggcacagat ccggtttccg gcaagaccat cgtggatgcc aaaagccagc gtttcttatt
     1441 ggccatgatg accatgtgcg gcttgtatga cgcctccgga gagtttgccg tacgcgtggg
     1501 tttaccggcc aaaagcggcg tgggcggggg tatttttacc gtcgtgcccg gccgttatac
     1561 gatcgtcaca ttcgggccgg ctttagatga taaaggcaac agcatcggcg gcattcaaat
     1621 attagaacac ctctcttcgg cctgccaact ttcctcctgg gtataagcat ttcccttgtt
     1681 attaacgact tcatacttga attttaattg gcttttagca aaaagcgttt ggtttgcaga
     1741 ctatgttgtg accgttatgt gacgcataac ataatatcga aatatccctt accatttctc
     1801 tttgcgactt ggcgaaaaaa aatattctcg ctaagacgcc aacccctacc ctgccgttcc
     1861 cgcgaaaggg gaatgaccgt tgtgtatgag cacccgcgtg cgcgggtgag acaatgtaaa
     1921 atcacacctg tttctatttt ctctttgcgg ctttgcgaga aattatacaa gctcgccaag
     1981 gcgctaaggc gcgaaaaaaa catcaagacc atataattga gtttcatgcg ttttaacgat
     2041 gcgctttggg gtcgctggtt acgttttgac cgttatgcga tcattaataa tttacgatat
     2101 atcgccaaaa acttaccaaa atcatttgac cgcgatgggt attttgccta tcttcggcgc
     2161 gcgtacagcc tatgtgattt tgcatatttt aacatattat ttcggaggat tttttaatga
     2221 atattatcgt ctgcatgaaa caagttccgg acagcgaaac gcgcgtaaaa atcggcgccg
     2281 acggcaagtc tgtggatctt tcggcggcca attttgtggt caacccctat gacgaatttg
     2341 ccatcgaaga aggcattcgc ctcaaagaaa aatttggcgg cgaagtgact atcttaactc
     2401 tcggtacaga tcgcgcggaa aacgatattc gtaaaggcct cgctatgggc gcggataaag
     2461 cgacgttact caaagccgat aattttaacg gcgatgtagc gcatgctctg gcggaagaaa
     2521 tcaaagccgg cgcttacgat ctggtgctct ttggcaaaca agccattgat gaagacagtt
     2581 ccgtcatgcc gcagattgtc gctgaaaagc tgaatctccc gtgcgtcacc gtagttacca
     2641 aactggatgt aaatgccgac accaaagcga tcacctgcga acgtgaaatc gaaggcggca
     2701 aagaaatcgt cgaactcact tatcctgccg tgatcggtac tcaaaaagga ctcaacgaac
     2761 cgcgtttgcc caacctcaaa ggcatcatgg cggccaagaa aaaaaccata gataaaaaag
     2821 atccgg
//
LOCUS       DAOQNQ010000374         2814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_19510_length_2814_cov_5.393621, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000374 DAOQNQ010000000
VERSION     DAOQNQ010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19510_length_2814_cov_5.393621"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PK754_15125"
     CDS             <1..603
                     /locus_tag="PK754_15125"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNB10883.1"
                     /translation="MRAPGMELFATPIHLMPQSIVRVTVWEDTCEAWDCGGAPAEWLS
                     QFLGARYRLVRMQPQFLRPVDRKYAVADDHVSFADAYPALVISEASLELLNSKLASPV
                     TMTRFRPNVVVSGCAAHEEDTWQIMRTGAVEFRGVKRCSRCVLTTVDPETTESGAEPL
                     KTLATYRRSAEGKIMFGMNLIPVQKNGKLRIGDTITYTGS"
     gene            606..1385
                     /locus_tag="PK754_15130"
     CDS             606..1385
                     /locus_tag="PK754_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020159668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNB10884.1"
                     /translation="MKITMYQVDAFTRKVFSGNPAAVCPMQVWPEDEIMRGIAAENNL
                     SETAFFIPQGDDFALRWFTPEVEIDLCGHATLATAHVLFEHLHFERSEIHFHTQSGKL
                     TIRRLPDGKLLMDFPARKPLPCTVPRVLVEGLGANPDVILKARDYFCVFDSEATVRAL
                     SPDFTMLKSLGEKVIVTARGDTTDFVSRFFAPSVGINEDPVTGSAHCSLIPYWAERLG
                     KDELYAHQVSKRGGEIFCKLRGDRVDIGGYAADYLQGTIQW"
     gene            1391..1816
                     /locus_tag="PK754_15135"
     CDS             1391..1816
                     /locus_tag="PK754_15135"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB10885.1"
                     /translation="MRIHFITLGVSDMQRAANFYSDVLGFKILKKTPVITFFDNGGMV
                     LGVYPIQQLNALTGASFETVSPSSTGILLSHNVQNENDVDAWVNKLKNEKVKILKEAQ
                     PAVWGGRHAFFSDPDGYSWEICWNPKLIPDEFEHLPLVN"
     gene            1879..2220
                     /locus_tag="PK754_15140"
     CDS             1879..2220
                     /locus_tag="PK754_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10886.1"
                     /translation="MLPLEGLIAWAVFIGMMVIAFKLAILVMIFVVMQSRQNQQRWRE
                     HRSFGYLYSCLVCFLIGSVGTMYMFTVSDKAELTFLDFYVSPIIFSVCCVLYFWIGYV
                     WKKKIQQPKAP"
     gene            2230..>2814
                     /locus_tag="PK754_15145"
     CDS             2230..>2814
                     /locus_tag="PK754_15145"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNB10887.1"
                     /translation="MVFELNKESDVPLYTQIERRMTELIRVGILKADEKLPATRDLAL
                     QLHVHRNTVVQAYQELEVKGFVYSEVGRGTFVSKHAHVAAKGTVAAQNKSGNFSFDGL
                     YAEHWINPEDPSMQAIDTIVRTCSKPQDMIAFSSVVPDKHFFPLVDFQNCSYRAIQKY
                     GVDLLEMGDTQGFRPFVEYLPKFLVRRGLTVQSSD"
BASE COUNT          695 a          600 c          747 g          772 t
ORIGIN      
        1 atgcgggcgc cggggatgga gctttttgca acgccgatcc atcttatgcc gcaaagcatc
       61 gtccgcgtca cggtatggga agatacgtgc gaagcatggg attgcggcgg cgcaccggcc
      121 gaatggctga gccagtttct cggtgcgcgc taccggttag ttcgtatgca accacagttt
      181 ttacgaccgg tggatcgcaa gtatgccgtt gccgatgatc acgtgagttt tgccgatgcg
      241 tatcctgcgc tggtgatttc ggaagcctcg ttagaattac tcaattccaa actggcttcg
      301 ccggtgacga tgacacgatt tcgcccaaat gtcgtcgtaa gcggttgcgc ggcgcatgaa
      361 gaagatacat ggcaaatcat gcgtaccggt gcagtagagt ttcgcggcgt caaacggtgt
      421 tcccgttgtg tactgacgac ggtggatccg gagacaaccg aaagcggtgc ggaacctttg
      481 aaaacactgg cgacatatcg ccggagcgcg gagggtaaaa ttatgttcgg gatgaatctt
      541 attccggtgc aaaaaaatgg taaactgcgt atcggcgata cgatcactta tacgggatca
      601 taaaaatgaa aatcacaatg tatcaggtgg atgcgtttac gcgtaaagtt ttttccggca
      661 atccggcagc ggtttgcccg atgcaagtct ggccggaaga tgaaataatg aggggtattg
      721 ccgctgaaaa caatctttcg gaaacggcgt tttttattcc gcaaggtgac gattttgcat
      781 tgcggtggtt taccccggaa gtggaaattg atctttgcgg tcatgccaca ctggcgacgg
      841 cccatgtgct ttttgaacac ctgcactttg aacgatcgga aatacatttt cacacgcaaa
      901 gcggaaaatt gaccatacgt cgtttacctg acgggaagtt gcttatggat tttcccgcgc
      961 gcaaaccctt accctgcacg gtgccgcggg tattagttga aggtcttggc gccaatccgg
     1021 acgtaattct taaagcacgc gattactttt gcgtatttga ctccgaagcg acggtgcgtg
     1081 cattgtcgcc ggatttcaca atgctaaaat cattaggcga aaaagttatc gtcacggcgc
     1141 ggggcgatac gacggatttt gtgtctcgat tttttgcacc gagcgtcggt atcaatgaag
     1201 accctgtcac cggttcggcg cattgttcgc tgatcccgta ctgggcggag cggctgggaa
     1261 aagatgaatt gtatgcacat caggtttcaa aacgcggcgg tgaaattttt tgcaaattgc
     1321 gcggtgatcg ggttgatatc ggtggttatg cggccgatta tctgcaaggt acgatccaat
     1381 ggtgaaaccg atgcgaatcc attttataac actcggtgtg tcggatatgc aacgtgcggc
     1441 gaatttttat tcggatgtat tgggattcaa aatattgaaa aaaacaccgg tgattacatt
     1501 ttttgacaat ggcggtatgg tactgggggt atatcccatt caacaactga acgccttaac
     1561 cggcgcgtcg tttgaaacgg tatcgccttc gtcaacggga atccttttat cgcataatgt
     1621 tcaaaatgaa aacgatgttg atgcgtgggt caataaacta aaaaacgaaa aggtaaaaat
     1681 actcaaagaa gcacaacctg ccgtatgggg cgggcgccat gctttttttt ccgatccgga
     1741 tgggtatagc tgggaaattt gttggaatcc gaaattaatt cccgatgaat tcgaacatct
     1801 tccgctggta aattgaacgc atgcgatgag atttagttat gttaatacag ccaatattca
     1861 ctaaaaattt ataattctat gttaccactc gaaggcctca ttgcgtgggc cgtttttatc
     1921 ggtatgatgg tcatcgcttt taagttggcg atccttgtga tgatttttgt cgttatgcaa
     1981 agccggcaga atcagcagcg atggcgcgaa catcgttcgt tcggctacct ctacagttgt
     2041 ttagtatgtt ttttgatcgg ttcggtcggt acgatgtaca tgttcaccgt aagcgataag
     2101 gcggaattga cctttcttga tttttatgtc agcccgatca ttttttccgt gtgttgcgta
     2161 ctctatttct ggatcggtta tgtttggaag aaaaaaattc aacaacccaa agctccataa
     2221 aggtgatgta tggtgtttga actcaacaaa gaaagcgatg ttcctcttta tacgcagatt
     2281 gaacggcgta tgacggaatt gatccgcgtc gggattctca aggccgacga aaaattaccc
     2341 gctacgcgtg atctggcttt acagttgcat gtgcaccgta atacggtcgt tcaggcgtat
     2401 caggagcttg aagttaaagg ttttgtgtat tccgaagtag gtcgcggtac gtttgtaagt
     2461 aaacatgcac acgttgcagc caaagggacg gtcgctgccc aaaacaaatc cggtaatttt
     2521 tcatttgacg gtttgtatgc ggaacactgg atcaatcccg aagatccttc gatgcaggcg
     2581 attgatacga tcgtgcgtac atgcagcaaa ccgcaggaca tgattgcgtt ttcttcggtt
     2641 gtgccggata aacatttttt tccgctcgtt gattttcaaa actgttccta tcgggcgatt
     2701 caaaaatacg gagtggatct gctggagatg ggtgatacgc aggggtttcg accgttcgtc
     2761 gaatatttgc cgaagttcct tgtgcggcgc gggcttaccg tgcagagctc ggac
//
LOCUS       DAOQNQ010000375         2809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_19580_length_2809_cov_5.855120, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000375 DAOQNQ010000000
VERSION     DAOQNQ010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19580_length_2809_cov_5.855120"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2809)
                     /locus_tag="PK754_15150"
     CDS             complement(<1..>2809)
                     /locus_tag="PK754_15150"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNB10888.1"
                     /translation="QTTNGYNLTLVGTPTFTASSSDSVMIRQQTKSLYTGGTSVSVGW
                     HADPHSFVTGYIIYADTSDAVRTQVGTTSSRTDTTFTVTGLLPNTVYYLTARAVTATD
                     TSDYYRLAVVSTFESSGRAAMFSDNNYYAAPFNENIPIGSSAYTIEAWIKPNSMGVHG
                     IVGWGQYGNSYQTNALRLTEDGIINYWWNNDMTISTGNIAGSWHHVAATYDGTTRKMY
                     FDGKLMASENVVYGPEIPFSHNFTIGVTNTSEYFNGEIDEVRIWNVARSESEIVSLLF
                     SPATGSESGLASVYHFDEIVGDYAGDYKNANHAEAVSGTPQRTLSDAMLPFMPARLQA
                     IAGDGQVELRWEKSIHPDFASYTLMAATDIEFTDIVATHSSSAGNASDTSHTVAGLTN
                     GTLYYFAVKMTTNSGATTPFRMNAAVPNTGGPGNALIFDGEDDQIILEDNIVRSRSEM
                     TIETWFKTTTSGGIFGYNDASNAEHVPVIYVGMDGYLQSQLWIGDYNSVMRSLRPVSD
                     GYWHHVALTAGNDNQYLYIDGQLCDSIIGRPLNHLSMIKNQIGRATTSGWPNGGNEYF
                     SGELDEFRFWNRALSHQEVYSYANKSLRGDENGLVLLHHFNEPSGLIYTYDAGPNGWT
                     GGLESFSASTTVASGALRPFSPYNLQSTNTLTSITLKWNRSNAASVRAYYIYTFDGES
                     TMLWDSTTGGLTDTTITFAELPSFTNFTYYLSTVDASGSESEKSNPIFVTTTSFKEVT
                     GTTFSQVVDGKFQWADYDNDGDYDVLVAGSENNGYYPPYQNGTIKLYRHDNGGTFTLV
                     DVGLTQEIHRGTAAWCDYNNDGLPDISYMGSGGNGTRPFKLYKNNGDGTFTEILTNIQ
                     GAIFGAMAWGDYDNDGDQDVFVMGSIGSEGGNTQSMLFRNDRNDLFTQVDIGISGYSG
                     GSAAWGDYDRDNDLDLLILGST"
BASE COUNT          725 a          655 c          683 g          746 t
ORIGIN      
        1 tggttgaacc taaaatcaaa agatcaagat cattgtctcg atcataatcg ccccaagcgg
       61 cagatccgcc ggaatatcct gaaataccga tatccacttg agtaaataga tcgtttctgt
      121 catttcggaa aagcatggat tgggtatttc cgccttcgct tcctattgaa cccatgacaa
      181 acacatcctg atcgccgtcg ttgtcatagt caccccaagc cattgcacca aaaatagcgc
      241 cttggatatt ggtcaggatt tctgtaaaag taccatcgcc attattttta tataatttga
      301 acgggcgtgt tccgttgccg ccgcttccca tataggaaat gtccgggagc ccgtcattgt
      361 tatagtcgca ccatgccgcc gtaccgcgat gaatttcctg agtaagaccc acatcaacta
      421 aagtaaatgt cccgccgtta tcgtggcgat acagtttgat cgttccgttt tgatagggag
      481 gatagtagcc gttattttcc gaaccggcga ctaatacgtc ataatcgcca tcgttatcat
      541 aatccgccca ttggaatttt ccgtcaacga cttgtgagaa agtcgttccg gtaacttctt
      601 tgaaggaagt tgtcgtcacg aaaatggggt tagatttttc cgactcggaa cccgatgcat
      661 ctacggtcga taagtaatag gtgaaatttg tgaatgacgg taattcggcg aatgttatcg
      721 tcgtgtcggt caatccgcca gtcgtagaat cccaaagcat tgtggattcg ccgtcaaaag
      781 tataaatgta gtaagcccgg acagacgcgg cgttactccg attccatttt aaggtgatgg
      841 aagttaatgt attggtagat tgtaaattat acggtgagaa tggtcgcaac gctccggatg
      901 ccaccgttgt tgatgcggag aaactctcca atccacccgt ccatccgttg ggcccggcgt
      961 catacgtata aatcaaaccg gagggctcat taaaatgatg caataggaca agcccgtttt
     1021 catctccgcg cagagacttg ttggcataag aataaacttc ttgatgtgag agggcgcgat
     1081 tccaaaaacg aaattcgtcc aactcgccgc taaagtattc attcccgcca tttggccaac
     1141 ctgaagtagt agccctaccg atttggtttt tgatcatgct tagatgattg agcggtcgac
     1201 cgataattga gtcacacagc tggccgtcaa tatataggta ttgattgtca ttaccggcag
     1261 taagcgcgac gtgatgccaa taaccatctg acacaggtcg aagtgatcgc atgacagaat
     1321 tataatcgcc gatccacaat tgagattgaa gatatccatc catgccaaca tagattacag
     1381 ggacatgctc tgcattcgag gcgtcattat aaccaaaaat gccgccggaa gttgtggttt
     1441 taaaccatgt ttcgatggtc atttcggacc gtgatcgcac gatattgtct tccagtatga
     1501 tctgatcatc ttcgccgtca aatataagcg cattgccggg accgccggta ttagggactg
     1561 ccgcattcat gcgaaacggt gtggttgcgc cggagtttgt tgtcatcttg acggcaaaat
     1621 aatacagcgt tccgttagtc agtccggcta cggtgtggga tgtgtcggat gcgttgccgg
     1681 cgctggagga atgcgttgct acaatatcgg taaattcaat atcggttgca gccatcaatg
     1741 tatagctcgc aaaatccgga tgaatggatt tttcccaacg caattcgacc tggccgtcac
     1801 cggctattgc ctgtaaacga gccggcatga aaggtaacat cgcgtccgac aatgttcttt
     1861 gcggagttcc gctgacggct tcggcgtgat tggcattctt ataatcaccg gcataatcgc
     1921 cgacgatttc gtcaaaatga tacactgacg cgagaccact ctcggaacct gttgccggtg
     1981 aaaacagtaa tgaaacgatt tcagattccg aacgtgcaac gttccatata cgtacttcgt
     2041 caatttcgcc gttgaagtat tcgcttgtgt tggtaacgcc gatggtgaag ttgtgagaaa
     2101 aggggatctc agggccgtac acaacatttt ccgaggccat gagtttgccg tcgaaataca
     2161 ttttacgcgt tgtaccatca taggtcgccg ctacgtgatg ccaacttccg gcgatattac
     2221 cagtggagat ggtcatatca ttgttccacc aataatttat aataccgtct tcggtaagcc
     2281 gcaatgcatt agtttgataa ctgttaccat attgccccca acctacaata ccgtgtacgc
     2341 ccatggagtt cggtttaatc caagcttcga tagtgtaggc gctgcttccg atggggatgt
     2401 tttcattaaa aggcgcggca tagtagttat tatccgaaaa catcgccgca cgcccgctgc
     2461 tttcgaatgt ggaaaccacg gccaaccgat aataatcgct ggtgtccgtg gcggtaacgg
     2521 cgcgcgctgt gagataatac accgtattgg gaagaagtcc tgtgacggta aacgtcgtat
     2581 cggtgcggga tgaagtagtg ccgacctgcg tccgtacggc atcggatgta tcggcataaa
     2641 tgatgtagcc ggtgacaaaa ctgtgcggat cggcatgcca ccccacggat accgatgtgc
     2701 cgccggtata tagcgatttt gtctgttggc gaatcatcac gctatccgaa gacgaagcgg
     2761 taaatgtggg tgtgccaact aaagtcagat tataaccgtt agttgtttg
//
LOCUS       DAOQNQ010000376         2796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_19740_length_2796_cov_5.403867, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000376 DAOQNQ010000000
VERSION     DAOQNQ010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19740_length_2796_cov_5.403867"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PK754_15155"
     CDS             <1..536
                     /locus_tag="PK754_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015333719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNB10889.1"
                     /translation="GGLAETLGDPYRHLSDKSLRSWKIERYSVYGVLIFVVITTAVVW
                     INSLSNGQLLGNVSSLFSNVYGFVIGALFSGVIGVGFYPLLGSRVWCRFGCPMAAYLG
                     ILQRYFSRFRITTNGGQCISCGNCSTYCEMGIDVRSYAQRGQNIVRASCVGCGICSAV
                     CPRGVLNLENGPIHSRQ"
     gene            742..>2796
                     /locus_tag="PK754_15160"
     CDS             742..>2796
                     /locus_tag="PK754_15160"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HNB10890.1"
                     /translation="MVQRPYTVASDGTGRYRTIQEAVDKSELNSIIRVKAGIYRENLI
                     LRNFISLEGDPAGRTLVIGSPTAPVIQAYNISSLRLRDLSFEFDQPTAFPVLLVRYSN
                     FSADRVAFRRGATGVEMKSNSTVTLRECSISDNTGYGISVSDRSQGTITQSFIYQNEA
                     SGISVSDKSSPLIERNIIRDNKKDGLLINLGSVGKVIGNTIYRNGENGITIDGQSNPL
                     IRNNTIVFHKNDTSRYAEKETGYGVLIRNASGLALINNNIVWNHIGIGQKQGTLALLS
                     NNNVWNNGVLYEGIEAHHTDVSHDPMFLNAAISDFRIDTTSKIYMAGEDRLSIGSHYD
                     YVRSDKKTRLDYLKTQAAKDLAKENWYQAFQNAKEILEIDKTDTEGKSLHKRAASQLA
                     DMYTKNARTEYEKDSYRIARGFLDMALGFDPEFADALELKQEIEEESRWINIRFTFVA
                     VSGCLVLAVLIFYMRKRSRRQELRRQAQWWVDDADENIVLARTAEAEMIASEDFKSAL
                     DKFSALQKAFADMQYGLCESLSAEVVRLAIRAKDSAERYKQLRKEALLEVSNAELDLK
                     NFSESEDGVSFPKEISEYRFYLERAQDALVQKHYVMAKEIADDIQRNTRDLRQRLQTQ
                     RETAVITLIQETETCIIEALAGHSSADITMAVMDFRSELQVLKSGFENGQLSVAEVTP
                     QVE"
BASE COUNT          767 a          566 c          672 g          791 t
ORIGIN      
        1 gtggcggttt agcggaaact ctgggcgatc cttacagaca tttatccgat aaatccttac
       61 gctcgtggaa gatcgaacgg tattcggttt atggtgtttt aatttttgta gtgatcacaa
      121 cagcggttgt ttggattaat agtttatcca atggccaatt actcggaaac gtatcatcac
      181 ttttttcgaa tgtgtacgga tttgttatcg gtgcattatt ttccggtgtg atcggcgtcg
      241 gtttttatcc tttgttgggc agccgtgtat ggtgccgttt tggttgccct atggccgcct
      301 atctcggaat attgcaaaga tatttttcgc gttttcgtat caccaccaat ggcgggcaat
      361 gtatcagttg cggtaattgt tcgacgtatt gtgaaatggg aattgatgtt cgttcgtacg
      421 ctcagcgtgg acagaatatc gtgcgagctt cgtgcgtcgg gtgcggaata tgttcagcgg
      481 tgtgcccaag aggtgtactt aatttggaaa acggaccgat tcactcccga cagtaatcaa
      541 acagtataca ttttttcact gaaccctcat taagttatcg tttaatgagg gtttaccatt
      601 tttggtgaat gtcagaaaat tttgatttac attacaaacc atatgccttt gatatcaaaa
      661 ataatgcgct ttacctgtgt atcggtcgtt cttttttgga cgcaggtgaa cggtcaagtt
      721 tcccgttcta cgaggagacc gttggtacag aggccctata ctgttgcttc ggatggcacc
      781 gggcgctacc gcacgataca agaagcggtt gataaatcgg aactgaattc gattatacgc
      841 gtcaaagccg ggatatatag agagaatcta atccttagga attttatatc ccttgaaggc
      901 gatcctgcgg gacgaacact cgtgatcgga tcaccaacgg ctcccgtcat tcaagcctac
      961 aatatcagct cacttcggct tcgtgatttg agttttgaat ttgatcaacc tacggcattt
     1021 cccgttttgc tggtgcggta ttccaatttt tcagccgatc gtgtagcttt cagacgcggc
     1081 gcaacgggcg ttgaaatgaa aagcaatagt acagtcacat tacgtgagtg cagtatcagc
     1141 gataataccg gttacggtat cagtgtatcg gaccgaagtc aaggtacgat cacacagtcc
     1201 tttatttatc aaaacgaagc ttccggaatt agcgtcagcg ataaatcatc gccgctgatc
     1261 gagcgcaata ttatccgtga taacaaaaaa gacggtttat tgataaatct cggatctgtc
     1321 gggaaagtca tcggcaatac catatatcgc aatggtgaaa atggaatcac tattgatgga
     1381 cagtccaatc cgttaatacg gaataacacc atagtctttc ataaaaatga tacgagccgg
     1441 tatgccgaaa aggaaacggg ttatggtgtt ttgataagaa atgcatcggg cctggccttg
     1501 atcaataata atatagtgtg gaatcatatc ggtatcgggc aaaaacaagg tacacttgct
     1561 ttgttatcca ataataacgt gtggaataat ggcgtattat atgaaggcat cgaagcccat
     1621 cacaccgatg tgagccatga tccgatgttt ttgaatgcgg cgatttccga ctttagaatt
     1681 gatacgacat ccaaaatata tatggccggc gaagaccgat tatccatcgg ttcgcattat
     1741 gattatgttc gcagcgataa aaaaacgcgt cttgattacc tcaaaacaca ggccgccaaa
     1801 gatctggcta aagaaaattg gtatcaggct ttccaaaatg cgaaagagat attagaaatt
     1861 gataaaaccg atacggaagg caaatctctt cataaacggg ccgccagtca actagccgat
     1921 atgtacacca aaaatgcgcg gacggagtat gaaaaagaca gttatcgcat tgcgcgggga
     1981 tttctcgata tggcattagg atttgatccg gaatttgccg acgcattgga actcaagcaa
     2041 gaaatcgaag aagagtcgcg ctggatcaat attcggttca cttttgtggc tgtttccggg
     2101 tgtttggttt tggccgtcct tattttttat atgcgtaagc gatcacgtcg tcaagagttg
     2161 cgccgacagg cacaatggtg ggttgatgat gccgatgaaa atatcgtatt agcgcgtacg
     2221 gctgaagccg aaatgatcgc ttcggaagat tttaaatccg ccttggacaa attctccgct
     2281 ttgcagaagg cttttgctga catgcagtat ggtttatgtg agtcattatc cgccgaagtg
     2341 gtgcgcctgg cgattcgcgc caaggattca gcggagcgtt ataagcagtt gcgcaaagaa
     2401 gcgttgcttg aagtgtccaa tgctgaactg gatttgaaaa atttttctga atctgaagac
     2461 ggcgtttcat ttccaaaaga aatttccgag tatcgctttt atttagaacg tgctcaagac
     2521 gctttagttc aaaagcatta tgtcatggcc aaagaaatcg ctgatgatat tcagcgaaat
     2581 actcgtgatt tacgccagcg cctgcaaaca caacgtgaaa cagcggtgat aacgctgata
     2641 caagaaacgg aaacctgtat catcgaagcg cttgccggtc actccagtgc cgatattact
     2701 atggccgtaa tggattttcg ttcggaactt caggtgctca aatccggttt tgaaaacggt
     2761 caactgtccg tcgctgaggt aacgccgcag gttgaa
//
LOCUS       DAOQNQ010000377         2745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_20446_length_2745_cov_9.073606, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000377 DAOQNQ010000000
VERSION     DAOQNQ010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20446_length_2745_cov_9.073606"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PK754_15165"
     CDS             <1..190
                     /locus_tag="PK754_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10891.1"
                     /translation="SALTAEQIKRQYNGGYGNPPLDVPAYLIHEYDGTGGTSTSETDQ
                     SGGGLTGTLNNGVTRTTW"
     gene            184..390
                     /locus_tag="PK754_15170"
     CDS             184..390
                     /locus_tag="PK754_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10892.1"
                     /translation="MVKIIIGFFIGVLLTLGVQNYLTKGDEKNEITIDEKLIDSLDND
                     TKRDFLEERARSRKERKKDMGKND"
     gene            594..953
                     /locus_tag="PK754_15175"
     CDS             594..953
                     /locus_tag="PK754_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10893.1"
                     /translation="MQFLIAGLFGALLYIIFSALKAHPKDSLDAFIGYFKKHPLAIGA
                     SYLTYALTMFAWYANGLDSAVSWVAGVFALSYDGQFFPQGKFNFLAIGIGFASHVIAN
                     MLYMKLTNYHSKQAEAQ"
     gene            950..1201
                     /locus_tag="PK754_15180"
     CDS             950..1201
                     /locus_tag="PK754_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10894.1"
                     /translation="MTAPKFDNKISWGNIIYLVGLIFAVAVFYAKTTANDKTLEKITP
                     EVENLKTETEVLKTELKSINKKLDEQNDLLRGLVFGKNR"
     gene            1536..2105
                     /locus_tag="PK754_15185"
     CDS             1536..2105
                     /locus_tag="PK754_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10895.1"
                     /translation="MTEQIRNINNTRLLSVDESIRDQVMMCLDRMRELEQPAVIHRDV
                     WRSPALQELKFRQGVSKLKWGYHCATRNGKPASLACDIVHIDHAWFSKFDATYKADEY
                     FWLCLGRSAKEAGLDWGGEWMWTWPSKEATEKVRKLNERIDTLDATGLETARDFGWDV
                     AHIEVRGVSAEEAAQGWRISNGVKFKIVP"
BASE COUNT          833 a          510 c          619 g          783 t
ORIGIN      
        1 atcagcatta acggcggagc aaataaagcg gcaatataac ggcggatatg gtaatccgcc
       61 gttagatgtg ccagcttatt taatacatga gtacgatggc acgggcggta cgtcaacatc
      121 tgaaacagat caaagcggcg gtggtttaac tggaacttta aacaatgggg ttacgagaac
      181 aacatggtaa aaataataat tggatttttt atcggcgttt tattaacttt aggggtacaa
      241 aattatttaa ctaagggaga cgaaaaaaat gaaatcacta ttgatgaaaa gcttattgat
      301 tctcttgaca atgatactaa gcgcgacttt ctcgaagaac gtgcaaggtc aagaaaagaa
      361 agaaaaaaag atatgggtaa aaacgattga cagcgtctta gtcagcgaag acgttttaaa
      421 ccaagccgtg aaagatgcta tcgttgcgga taaatgcggc aaggaaatcg aaaggcaaaa
      481 aaaacaagct cgcactaacc gaatacgcgg ttggatagag ggttcggcgg tgtttctaac
      541 ttacatgatg tttaaatatt ggaaaaaatg aggtgcaaca gatgagcgca aacatgcaat
      601 ttttaatcgc ggggcttttc ggggcattgc tttacatcat attttcagca ttaaaggcgc
      661 acccgaaaga ttcgcttgat gcgtttatcg gctatttcaa aaaacatcca cttgccatag
      721 gcgcgtcgta tttgacgtat gcgctgacaa tgttcgcatg gtacgctaac ggcttagatt
      781 cagccgtgtc atgggtggcc ggagtgtttg cgcttagtta tgatggacaa ttttttccgc
      841 aaggtaaatt taatttttta gctatcggta tcggctttgc ctcgcacgta atcgcaaaca
      901 tgctatacat gaaattgact aattatcatt ctaaacaagc agaggcgcaa tgaccgcacc
      961 gaagttcgat aacaagatta gctggggaaa tattatttat cttgtaggtc ttatctttgc
     1021 cgtcgccgtg ttctacgcga aaacaacggc taacgataag acgcttgaaa aaattacgcc
     1081 ggaggttgag aatttaaaaa cagaaacgga agtattaaaa acggaactta aaagtataaa
     1141 caaaaaactt gacgagcaaa acgacttatt gcgcgggctt gtatttggaa agaacaggta
     1201 acaatgataa actttattat taaatttcaa aatgctattg gccatattat cggcatcgtt
     1261 gcaacggctt tgataatcat gcaggtcccg gtgtatatgc cagcgtattt atatttttcg
     1321 cttgtagctt tcgttgtatg gattatgttt gaacttgtgc aagcggaggc ggcaatacga
     1381 agtgattatt ttaagaacag aaagaccgcg tcgttgttta cgtgtatatt gcgctatcaa
     1441 tggaaagaaa gtttattcaa ggatgtatta ggcaagaatt tatacgggct tggcttgtct
     1501 ataatcgttc ttggatttat tacgggctgg aaataatgac cgaacaaatt cgcaacataa
     1561 acaatacgcg cctattaagc gttgacgaaa gcatacgcga tcaagtgatg atgtgccttg
     1621 atagaatgcg cgaacttgaa caacccgccg tcattcatcg tgatgtatgg cggtctcctg
     1681 cattgcaaga gcttaagttc cgtcaaggcg tttctaagct aaaatggggc tatcattgcg
     1741 ccacacgtaa cggaaaaccc gcttccttag cttgcgatat tgtacacata gatcacgctt
     1801 ggttttctaa atttgacgct acatacaaag ccgatgagta cttttggctg tgcttaggtc
     1861 gttccgctaa agaggctggg cttgactggg gcggcgaatg gatgtggacc tggccatcaa
     1921 aagaggcgac ggaaaaggta cgtaaactaa acgaacgcat tgatacgctt gacgctacgg
     1981 ggcttgaaac agcgcgtgat ttcggctggg acgtagcgca tatcgaagtg cgtggggtaa
     2041 gcgcagagga ggcggcgcaa ggttggcgca taagcaatgg tgttaagttt aagatagtac
     2101 cgtgattatg atatagtagt tatgaccaat gctgggcgat caatagcctt gtttgtttgt
     2161 ctgaaagaaa aggcttttta ttatcatggt aatcaggctt aaatgcttcg gcaagccatt
     2221 tagatttaac aaataaaatc acctgcgcat ataacactcg cacatgccgt gttgattatc
     2281 aaagtaaatt ccgctcttac cttcagttat ttcagggttc aaacacaccc atccacaact
     2341 attcattata ctaccttcac caaatccatt attcatagga tgtctgcgta attctatttg
     2401 atgtttacaa ttacaacaac atgaaccatc atcccatcca taagaacatt ttgtcgcgcc
     2461 taacgtagta tttatggcat cggagggggg tgctttgtag cattggtcat ccggttgtgt
     2521 cgtttctact ccgcaatatt tacaagtgtg gagtaaacta tcaaataatg cgccattttc
     2581 gattcccatt tttctttcat ccttccaaaa tatatgaggt gtgccaatct gcaagcgttt
     2641 gttgtatatg tttactttta tgatacatat acgatagtta tacgcaagca ctacatttcg
     2701 tttccaaaga gagttttcgg ttcaaaattt ttattaaaat ctgcc
//
LOCUS       DAOQNQ010000378         2742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_20499_length_2742_cov_6.429847, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000378 DAOQNQ010000000
VERSION     DAOQNQ010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20499_length_2742_cov_6.429847"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..805
                     /locus_tag="PK754_15190"
     CDS             152..805
                     /locus_tag="PK754_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNB10896.1"
                     /translation="MSKIRVESFTISLDGFGAGIEQTMSNPMGVGGQNLHQWAIPTKT
                     FQKALFGSDNGTTGIDDKFATRGFDNIGAWILGRNMFGPIRGEWPDMSWKGWWGENPP
                     YHVPVFVLTHYPRPSITMEGETVFHFVTGGIHEALDMARQAAEGKDVRIGGGVQTIQQ
                     YLRAGLIDEMHFAISPVFLGRGERLFSDVDLVALGYRCIECTSTEKATHVIVQRQNQ"
     gene            complement(1162..2205)
                     /locus_tag="PK754_15195"
     CDS             complement(1162..2205)
                     /locus_tag="PK754_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNB10897.1"
                     /translation="MKKNQVLFFCFFVALLDGCAPKSKNIWVIQEAVTKERLRGISAV
                     SERVVWASGNHGTCIRTTDGGSSWEKINIPNSDSLDFRDIEAFDADTAYVLSIGYGRQ
                     SRVYKTTDGGKSWQLQFLNTDTTAFFDAFAFWNSQDGIAVSDAVDGRWILVSTHDGGT
                     SWNRFPSQQSPLALSGEGAFAASGTCITTYGDSMVWIGSGVNAARVLSSNDRGKNWRY
                     SETPVAHSNGTSGIFSIAFYDEKKGVVCGGDFKDEKNNTNSLAFTADQGITWQIVNNK
                     LPQGFRSSILWLTDKILIAVGPNGSDISYDSGMTWNFLGDTGFHTADAGLRSKTIWAA
                     GDSGRVGKLDLDM"
BASE COUNT          806 a          549 c          549 g          838 t
ORIGIN      
        1 tgcgatgcca agtaaagtca ggctggcaaa ttgaaaaatg tatgcttcca tgtgtttatc
       61 cggccttagc cgttttgggt tttatttcaa tcaaagcttg acaataaaaa caaatttacc
      121 tcaattaatt aaaattcaaa ggaagaaaaa tatgtctaaa atacgtgtag aaagtttcac
      181 tatttcattg gacggattcg gcgccggcat agagcagacg atgagtaatc ctatgggggt
      241 gggcgggcaa aatctgcatc aatgggcgat accgacaaag acatttcaaa aagcgttgtt
      301 tggttccgat aacggcacta caggaattga tgacaaattt gcaacacgag gttttgataa
      361 tatcggtgcg tggatactgg gtcgaaatat gtttggacct attcgcggcg aatggcctga
      421 catgtcgtgg aaaggctggt ggggcgaaaa tcctccgtat catgttccgg tgtttgtttt
      481 gacgcactac ccgcgtccgt caattacgat ggagggagaa accgtatttc attttgttac
      541 gggtggcatc catgaagcgc tggatatggc gcgtcaagcg gccgagggaa aagatgtgcg
      601 tatcggcggc ggtgtgcaga ccatacagca atatctccgg gccgggttga ttgatgaaat
      661 gcactttgcc atctcgcctg tttttttagg tcgtggtgag cgtttgttta gcgatgtaga
      721 tttggttgct ttgggttatc gctgcataga atgtacttcc acagaaaaag caacgcatgt
      781 cattgttcaa cgtcaaaacc aatagacgtg accgttaagt aaaattcagc tctatttcat
      841 agttatccat gacctcataa ttgaatcgat atgtgtttta aagacgagat attgattcgt
      901 tgactacgtt attaataatt ttgcaatcct gatatatcaa aaatctcttt tccatttctc
      961 ttagcggctt tgcgagaaaa tatatgtact agctaaagcg caaaggtgca aaaaattatc
     1021 catgacctca tggttgaatt gatatgtgtt ttaaagacaa gatagtgatt cattgactac
     1081 gttattgata attatgcgac tattaataat tgaattaact attagtcaaa gattttctgc
     1141 ctgttatgca attcgtaaac cctacatatc caaatctagt tttcctacac gtccgctgtc
     1201 tccggcggcc catattgttt tgctgcgcaa accggcatcg gccgtatgaa aacccgtatc
     1261 acctaaaaaa ttccaagtca ttcctgaatc ataagaaata tccgaaccat taggccctac
     1321 ggcgatcaat attttatctg tcaaccaaag tatggaagat cgaaaaccct gaggtagttt
     1381 gttgtttacg atttgccagg taataccctg atcggcggtg aaagcaagtg agtttgtatt
     1441 gtttttttca tctttgaagt ctccgccgca taccacacct tttttttcat cataaaaagc
     1501 gatggaaaaa atccctgaag taccattgga gtgtgcgact ggcgtttctg aatatcgcca
     1561 gtttttccca cgatcatttg acgaaagtac gcgtgccgca ttgactccgc tccctatcca
     1621 aaccatcgaa tccccgtatg tcgtaatgca tgtcccgctt gctgcaaacg caccctctcc
     1681 tgaaagtgct aacggcgatt gttgagatgg aaagcgattc cagctagttc ccccatcgtg
     1741 tgtagatacg agtatccagc gtccatccac tgcatcactg acggcgatgc catcctgtga
     1801 gttccaaaac gcaaaagcat caaaaaaagc cgttgtatcc gtgtttagaa attgcagttg
     1861 ccacgatttt ccaccatcgg ttgtcttgta aacacgtgat tgacgtccat aacctatgga
     1921 taaaacatac gccgtatcgg catcaaacgc ttcaatgtca cgaaaatcaa gcgaatcaga
     1981 attcggaata tttatttttt cccaagacga tcctccgtcg gttgtacgaa tacacgtccc
     2041 gtgattgccg ctggcccaaa caacgcgctc ggagacagcg cttattcctc gtaaacgttc
     2101 tttagttaca gcctcttgta taacccaaat attttttgat ttgggtgcgc atccgtctaa
     2161 taaggctaca aaaaagcaaa aaaataatac ttggtttttt ttcatacgtt ttctaacaga
     2221 tttagtaagt cctaagtaag attaatttag cgctgaaagg atatctccaa tctatatttt
     2281 ttgagattgc aatacaatga gcttcggctc ccttttacaa tgatttagtg atttttaaat
     2341 aatatttttt gttatttagg atagttgact gattaaaaaa attaaagtaa aatacttagt
     2401 tataaaatat tagaaaaatt cgtatcattt gagtacaatt atgaggtatt ttatatcaaa
     2461 attccaacta tgaaacagcc ttgtgttgta tttattaggt ttacgcttgg cacataaaat
     2521 gcaaatttac aaaagctttt aatgaattta cttaaggtct attttttata aaaactttta
     2581 ttttcaaaca aataagttta gagacatgaa aaaattttta ctccccattg tgtttttgct
     2641 cggctgcatc agcgcatcag cgtatgcatc ggcggaaaat aaaaccttag cctcttggtc
     2701 gaagtcaaat ctcaattcga tcaaacccat ggctgtccaa ac
//
LOCUS       DAOQNQ010000379         2736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_20585_length_2736_cov_7.484894, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000379 DAOQNQ010000000
VERSION     DAOQNQ010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20585_length_2736_cov_7.484894"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PK754_15200"
     CDS             <1..515
                     /locus_tag="PK754_15200"
                     /inference="COORDINATES: protein motif:HMM:NF015665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HNB10898.1"
                     /translation="DTMALAKTAEEVGVQNLVVNAAIQANDYQKMLMVEKIRKMVGGN
                     FKGKTIAVLGIAFKPNTDDIREATSLVIMEEITKEGGVVKAYDPVAMEQAQKFFPTMH
                     YAKDAFEAVQGADCMVIVTEWNEFRELDLVKAKTLLKTAKIVDCRNIFDPKEIKALGF
                     EYDAVGRITK"
     gene            548..1870
                     /locus_tag="PK754_15205"
     CDS             548..1870
                     /locus_tag="PK754_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNB10899.1"
                     /translation="MNLESKIKNKSAKVGIIGLGYVGLPLAVEFAASGYDTLGIDLDS
                     KKVKSINDGHNYIEDIPNDRFQKCYQSGKLKAETTYQRIGEMDIIYICVPTPFTDNKE
                     PDVSYIVSASEGIASGLRKEQLVILKSTTFPETTEKVTMPILDKKGLKVGADYYLAFS
                     PERIDPGNKKYTTANTPVVVGGVTSKCTELACLINAQIIEKVVPVSNPRVAEMCKLLE
                     NIFRSVNIALVNELAQLCDRMGGVDMWEVINAAGTKPFGYMPFYPGPGIGGHCILIDP
                     YYLSWKARGYDFHTNFIELAAEVNENMPYYAYSMIVEALANRGVALKKAKVLVLGAAF
                     KKDVDDTRHSPSLKVMEIMIQKGFSDVMYNDPYVPEVELLGKVYKSQAINTELLKSVD
                     VVFVGTDHSRYDYNEIVSHAASVVDSRNATKSVTHGRDKIAVLGSGKV"
     gene            1878..>2736
                     /gene="wecB"
                     /locus_tag="PK754_15210"
     CDS             1878..>2736
                     /gene="wecB"
                     /locus_tag="PK754_15210"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNB10900.1"
                     /translation="MKLLCIAGARPNFMKIAPILRAIKNYPQITSKLIHTGQHYDAVM
                     SDIFFKELQMPDPDIHMGVGGGSHAQQTADLMLKFDSILVNEKPDYVLVVGDVNSTMA
                     CAVTASKLHVPVIHVEAGLRSFDRTMPEEINRLVTDAISDYLFTTESSANQNLKNEGK
                     QENQIFFAGNVMIDSLTFALPKIEASDILQRMNLKEKEFVLVTLHRPANVDSEENLRS
                     MLGLLEFIAGYMPVVFPVHPRTKTKLTQYGMDHKVKLNERIRLIEPLGYFDFIALMRS
                     SVAAVTDSGG"
BASE COUNT          778 a          536 c          663 g          759 t
ORIGIN      
        1 aagataccat ggcgttagcc aaaacagcgg aagaagttgg tgtacaaaac cttgttgtca
       61 acgccgccat tcaggcgaat gattaccaaa aaatgctcat ggttgaaaaa atcagaaaaa
      121 tggtcggcgg taattttaaa ggtaaaacca tcgcggtgct gggtattgcg ttcaaaccta
      181 ataccgacga catccgtgaa gctacatcgc ttgtgatcat ggaagaaatc accaaagaag
      241 gcggcgttgt gaaagcttat gatcccgtag ctatggaaca agcgcagaaa tttttcccga
      301 cgatgcatta tgctaaagat gcttttgaag ccgtgcaagg tgctgactgt atggtgatcg
      361 tgacggagtg gaatgagttt cgcgagttgg atttggtcaa ggcgaaaaca ctgctaaaaa
      421 cggctaagat tgttgattgc agaaatatat tcgatcctaa agaaataaaa gcgctcggat
      481 ttgagtacga tgcggtgggg cgtatcacaa aataagttat taaaacaaca tttgttggag
      541 tgtccacgtg aatctagaga gtaaaataaa aaataaatcg gccaaagtgg gtatcatcgg
      601 attaggttat gtggggttgc ctttggctgt tgaattcgct gcctccggtt acgacaccct
      661 gggcattgat cttgattcta aaaaagtaaa aagtatcaat gatggccata actatatcga
      721 agatataccc aatgatcgtt tccaaaaatg ctatcaatcg ggtaagttga aagccgagac
      781 cacttatcag cgtattggcg aaatggacat catctatata tgcgtaccga cgccctttac
      841 ggataataag gaacctgatg tttcgtacat cgtcagtgcc agcgaaggta ttgcaagcgg
      901 gcttcgtaaa gagcagcttg ttattcttaa aagcactacg tttccggaga ccacggaaaa
      961 agtaacgatg ccgattcttg ataaaaaagg tctgaaagtc ggtgccgatt attatctcgc
     1021 attttcgccg gaacgcattg atcccggcaa taaaaaatat acaacagcca ataccccggt
     1081 cgtggtcggg ggtgtgactt cgaagtgtac cgaactggct tgtctcatca atgctcagat
     1141 catagaaaaa gtagtacccg tatccaatcc tcgggttgcc gaaatgtgca agctgctcga
     1201 aaatattttt cgcagtgtaa atattgccct tgtcaatgaa ttggcacaac tctgcgatcg
     1261 catgggcggt gtggacatgt gggaagtaat aaatgcggcc ggtactaaac cttttggtta
     1321 tatgccgttt tacccgggcc ctggtatcgg aggacattgc attcttattg atccctatta
     1381 tttgtcatgg aaagcacgcg gctacgattt tcataccaac tttatcgaat tggctgcgga
     1441 ggtcaatgag aatatgccct attatgccta ttcgatgatc gtagaagctt tggccaatcg
     1501 cggtgttgcg cttaaaaaag ctaaagtatt ggttctcggt gcggccttca aaaaggatgt
     1561 ggatgataca cggcattcac catcgctgaa agtgatggaa atcatgattc agaaagggtt
     1621 tagcgatgtg atgtacaatg atccgtatgt tcctgaagtt gaacttttgg gtaaagttta
     1681 taaatcacaa gctatcaaca cggaactttt gaagtccgtt gatgttgtgt ttgtcggaac
     1741 ggatcactcg cgctatgatt ataatgaaat agtctcccac gccgccagtg ttgtggattc
     1801 ccgtaatgca accaaatccg tgacgcatgg gcgcgataaa atagctgtac tcggctccgg
     1861 taaggtataa atcaacgatg aaactgctct gtatcgccgg tgcgcgtcct aattttatga
     1921 aaattgctcc cattctaaga gcgattaaga attatccgca gatcacatca aagcttattc
     1981 atacgggtca gcattatgat gcggtcatgt cggatatttt ttttaaagaa ttgcaaatgc
     2041 ccgatccaga tattcatatg ggagttggtg gtggatcgca tgcgcaacag actgcggatc
     2101 tgatgctgaa gtttgattcc attttggtaa acgaaaaacc cgattatgtg ttggtggtgg
     2161 gagatgtgaa ttcgacgatg gcgtgtgctg taacagcttc caaacttcat gtgcctgtta
     2221 ttcatgttga agccggatta cgaagttttg atcgtacgat gccggaggaa attaatcgtt
     2281 tggtaaccga tgccatttcg gattatctgt tcactaccga gtcatcggct aatcaaaatc
     2341 tcaagaatga gggtaaacaa gaaaatcaga ttttcttcgc cggcaatgtg atgattgatt
     2401 cgcttacatt tgcattaccc aagattgagg cgtcggatat tctccagcgc atgaatctaa
     2461 aggaaaaaga atttgtcctt gtcacactgc atcgtccggc gaacgtggat tccgaggaaa
     2521 atttacggtc tatgcttggt ctattggagt ttatagcggg ttatatgccg gttgttttcc
     2581 ccgttcatcc acggaccaaa accaaactta cgcaatatgg gatggatcat aaagtcaagt
     2641 tgaacgaacg aattcgattg attgagccgc tcgggtattt tgattttatt gcattgatgc
     2701 gttcctctgt tgccgccgtt accgattccg gcggtg
//
LOCUS       DAOQNQ010000380         2725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_20730_length_2725_cov_4.383521, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000380 DAOQNQ010000000
VERSION     DAOQNQ010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20730_length_2725_cov_4.383521"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..>2725
                     /locus_tag="PK754_15215"
     CDS             31..>2725
                     /locus_tag="PK754_15215"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNB10901.1"
                     /translation="MTSSRGYVTVIKQEPGELIYEFSIPSPDIDSVIGDDKALHSVAL
                     KHAESYLQADGYAVPSLNYWIELPVQANPTVRATAMNKKTLHGIRLSSARDDWSADSQ
                     ADPTSGLRSSRNSSTSALASIRYHGKMRDIPLYKLNITPIQYNAQQHTLEITTRMQVV
                     VRYNANTRTPVQEINNGDIDALLSDRLINAEPARRVRQLQTAASSTSIKLSKSSSSSS
                     ATAAITGGPYIRMTVDKEGIYKISVRELQDLSGVNLQSVDPRTLRLIGQGLEERVYVR
                     SKKTDRLAADDIIEFYGEPYRAKHNKYLRDIPSKQGHYLDPWATDNVYFLIWGSSAGL
                     RLIEEEGSLQERDPLSVIKPASYTTTKHFEEDNVLYDVKDINLIRPVAEEDIWVYDNG
                     ISYSAAVGSSTTSREYEFELPGVPTGTIQQFSLRINLQAISTQKHFVNVELNNNLITP
                     NPITWTDATKLQQDIPFSSDLLSPTGTNTLRISTTNSTERNSDIFAMNWFEITYKRNY
                     SAADNILIFTPDVPISGTTLHEFTLSGFNDLNISIYKRSSSRIVNPVIRNTSIQGKTT
                     YQALFQDDVPISGIEYIAVGETAKLTPKTVTAYNPGNLQTGNHDARYLIITSSALRDS
                     ALRLENYRKGQGYSAETITIEDIYDEFSYGAPGPYAIRDFLRYVYSAANWKGSQGNPL
                     YVCLIGDASRQPKTSSNEIVPTQFIQTQKYGAASSDYWFCLADDQDIIPDFFIGRIPV
                     INATQLNAYIDKIIAYEKGPSGNWKNKIVFIGGSEEQRATLGNPEIPRDVFRYQSTLL
                     INNRLAQRFTPERIYAYPGQEYGGGAGAVTDFFSDGALIITYLGHGGGGVWGDRDIAG
                     EPMMNHDRINLITPNGGRYPIVLSMTCFVGAFDSDGQFALGE"
BASE COUNT          753 a          695 c          590 g          687 t
ORIGIN      
        1 acaacttttt taactgcaca aacgacagac atgacttctt ctcgcggtta tgtcaccgtc
       61 ataaaacaag agccgggcga attaatctat gaattctcta ttccatcacc ggatattgac
      121 tcggttatcg gcgatgataa agccttgcac tcggtcgcac ttaaacacgc tgaatcgtat
      181 ttacaagcgg atggctatgc cgtaccctcg ttgaattatt ggattgaact gcctgtgcaa
      241 gccaatccca cggttcgcgc tacagcaatg aacaaaaaaa cactgcacgg tattcgcttg
      301 tcttctgcgc gcgatgattg gtcggccgat tcgcaagccg atcccacgtc cggactccgg
      361 tcgtctcgca attcttctac ttcagctttg gcatccatcc gttatcacgg taaaatgcgc
      421 gacattccgc tgtataaatt gaacatcacg ccaattcagt acaatgccca acagcacact
      481 ttggaaataa cgacccggat gcaagtggta gttcgttata acgccaacac tcgtacaccg
      541 gttcaggaaa taaataacgg ggacattgac gctttgctga gcgaccggct gatcaatgcc
      601 gaaccggcac ggcgcgtacg ccaacttcag acggccgcat cttccacgtc aattaaactt
      661 tctaagtctt cttcttcttc ctccgcaacg gcagcgatta caggcggccc atacatccgc
      721 atgaccgtgg acaaagaagg catttataaa atatccgtcc gagagcttca ggacttatcc
      781 ggcgttaacc tccaatccgt agatccgcgc acattgcgtt tgatcggcca gggtttggaa
      841 gaacgcgtat acgttcgttc caagaaaacg gatcgccttg cagccgatga tattatcgaa
      901 ttttacggtg aaccttaccg cgcaaaacac aataagtatc tgcgtgatat tccgtccaaa
      961 caaggccatt atcttgatcc atgggctacc gataatgttt attttttgat ttggggaagt
     1021 tctgccgggc tgcgcctcat cgaagaagaa ggttctcttc aagagcgcga tccattgtct
     1081 gtgatcaaac cggcttctta tacgacgaca aaacatttcg aagaagataa tgtactctac
     1141 gatgtcaaag atattaacct gatacgcccc gtagcggaag aagatatttg ggtatatgac
     1201 aacggcatat cgtattctgc agcggtaggt tcttctacga catcgcgcga gtacgaattt
     1261 gaattgcccg gcgtccctac gggaacgatt cagcaattta gcctgcgcat caatctacaa
     1321 gccatttcta cacaaaaaca ttttgtaaat gtcgaattga ataataatct gatcacaccc
     1381 aaccccatca cgtggaccga cgctaccaaa cttcagcaag atattccttt ttcatcagac
     1441 ctattatcac cgacaggtac aaataccctc cgcatctcaa cgacgaacag caccgaaaga
     1501 aatagcgata tcttcgctat gaactggttt gaaatcacat ataaaagaaa ctattccgcg
     1561 gctgacaata tactgatttt cacgccggat gtacccattt ccggtacgac attacacgaa
     1621 ttcacactca gcggttttaa cgatctcaat atcagtattt acaaacgcag ttcaagccgc
     1681 atcgttaacc ctgttattcg taatacgtcc attcagggta aaactacata tcaggcatta
     1741 tttcaagatg atgtaccgat atccggtatc gaatatatcg cggtcggtga aacagccaaa
     1801 ctaacgccta aaaccgtgac agcctataat cccggtaact tacagaccgg caatcatgat
     1861 gcgcgatatc ttatcattac cagtagtgca ctacgtgata gcgcactgcg actggagaac
     1921 taccgcaagg gccaaggcta ttcagcggag acaattacta tcgaagatat ttatgatgaa
     1981 ttcagctatg gcgcccccgg accgtatgcc attcgcgatt ttctgaggta tgtctattcc
     2041 gctgcgaact ggaaaggctc acaaggtaac cccctatacg tatgcttgat cggcgatgca
     2101 tcccggcagc ctaaaacaag ttcaaacgaa atcgttccga cgcaatttat acaaacacag
     2161 aagtacggtg cggcatccag cgattattgg ttttgcctcg ccgatgatca ggatatcata
     2221 cccgattttt ttatcggacg cattccggtg atcaatgcga cacaacttaa tgcctacatt
     2281 gataaaatca ttgcgtacga aaaaggcccc tcgggcaatt ggaaaaataa aattgtgttt
     2341 atcggtggca gtgaggagca acgcgcgacg ttgggaaacc ctgaaattcc gcgcgacgta
     2401 ttcagatacc aatccacttt acttatcaat aaccgattgg ctcagcgttt tacacccgaa
     2461 cgcatctatg cttaccccgg ccaagaatac ggaggcggtg cgggcgctgt aactgatttt
     2521 tttagcgatg gtgcacttat cataacgtac ctcggccatg gcggcggcgg ggtgtggggt
     2581 gaccgcgata ttgccggtga accgatgatg aatcacgacc gcatcaatct gatcacgcct
     2641 aacggcgggc gttatcctat cgtcctgagc atgacttgtt ttgtcggcgc ttttgacagc
     2701 gacggtcagt ttgctttggg cgaaa
//
LOCUS       DAOQNQ010000381         2717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_20829_length_2717_cov_6.243802, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000381 DAOQNQ010000000
VERSION     DAOQNQ010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20829_length_2717_cov_6.243802"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1356
                     /locus_tag="PK754_15220"
     CDS             <1..1356
                     /locus_tag="PK754_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10902.1"
                     /translation="YRLEFGQGVALWSGSGMSKSTEVIQSVKRRSGGIKPFASATENQ
                     ALQGIAAELQFEDVNFLHALKLTGFYSYAHFDASRNPDGTTGGLDESGLHRQTSENRR
                     RDYLLETLGGLHADYQINSATRLGATYYAHQFNRRFVPNDPVRNRYDFEGHRNRVGSI
                     SYDYYYEHVNFFGEWAMDGGADKAGNAGVQFFWPHMEFVFFYRNYAKNFHSLRGYAFG
                     EQNGRTRNEEGIYTGWKYEWRRRLTIQAYYDLYRFPWRTYDVPTAVTGRDFLTQADFA
                     LTRAMTITMLYKREEKDDAIQSTDILGRDIIIVEPSRVQRLRIQMEMLATPQFRFRSR
                     IETSYYAIPSMSGTSEKGLLIYEDVRFQPDRRWTLYGRVSVFDADSKSAVYEYENDVE
                     GAFANTRFSGKGLRWYVFTQYHGLNRMDIGFKYWQMARSDISGYGRMTRRVTLSVDYK
                     L"
     gene            1483..2109
                     /locus_tag="PK754_15225"
     CDS             1483..2109
                     /locus_tag="PK754_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNB10903.1"
                     /translation="MEEKTLVGYFTDGGFFMWPILICFIGGLAICIERLITLTRAATN
                     TKQFLVKIKQSLDEGGVERATEVCASQRGSVALIFHAGLLRARKGVEHVEKAIVNAGA
                     VEMAFLERGMVWLGFFVGAAPLLGFTGTVQGMIEAFDAIAKANDISPTIVADGIRTAL
                     YTTLFGLMVAILLQFFFNYFTAKINRIVADMEESSSELVDYLLENEVR"
     gene            2128..2529
                     /locus_tag="PK754_15230"
     CDS             2128..2529
                     /locus_tag="PK754_15230"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNB10904.1"
                     /translation="MFVEKRRKYTPEISTASMADIAFLLIIFFIVITKFGTDKGIDLT
                     LPPEGSIKEIPKNNIMSITMDDAGAVVLDGKAVILGQVQSLVKTIAEANDRLVITVKT
                     TRKTPYRNFIAVMDQLKEAGVRKISVLEPDR"
     gene            2531..>2717
                     /locus_tag="PK754_15235"
     CDS             2531..>2717
                     /locus_tag="PK754_15235"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNB10905.1"
                     /translation="MKFRRRPIESLPVSSASMSDIVFSLLLFFMVSTVIKKYSGLPVQ
                     VPAAFNIEKLSTKTHTSY"
BASE COUNT          702 a          575 c          689 g          751 t
ORIGIN      
        1 tatcgtttgg aatttggcca gggtgtcgcg ctgtggagtg gaagcgggat gtccaaaagt
       61 accgaagtca ttcaaagcgt aaaacgccga agcggaggga tcaaaccatt tgcttcagca
      121 acagaaaatc aggctttgca aggtatagcc gccgaattgc aatttgagga tgtgaatttc
      181 ttacacgcac tcaaactgac gggtttttat tcgtatgcac actttgatgc atcgcgcaat
      241 ccggacggaa cgaccggcgg tttggatgaa agcggattgc atcgccagac atcggaaaat
      301 aggcgcaggg attatctttt agaaacattg ggcggactcc atgccgacta tcaaatcaac
      361 agcgctacac gattgggagc gacgtattat gcgcatcagt ttaatcgccg gtttgtgccc
      421 aatgacccgg tacgcaatcg ttatgatttt gaagggcatc gcaatcgagt cggcagtata
      481 tcgtatgatt attactatga gcatgtcaat tttttcggcg agtgggcgat ggacggcggt
      541 gcggataagg ccggtaatgc cggcgtacaa tttttttggc cgcacatgga gtttgtattt
      601 ttttatcgta attacgctaa aaattttcat tcccttcgcg gatatgcttt cggcgaacaa
      661 aacggccgca cgcgtaatga agaaggtatt tatacgggat ggaaatacga atggcgtcgt
      721 cgcctgacca tacaagcgta ttacgatttg tatcgttttc cgtggcgtac ctacgatgtg
      781 ccgacagccg taaccgggcg cgatttcctg acgcaagcgg attttgcttt gacccgcgca
      841 atgaccatca caatgctcta caaaagggaa gaaaaagacg atgcgattca gagcacggat
      901 attttagggc gcgatattat catcgtcgaa ccgtcacgtg tgcaacgtct gcgcatacag
      961 atggaaatgc ttgcaacgcc gcagtttcgg tttcgttcac ggattgaaac atcctactac
     1021 gccatacctt ccatgtcggg cacttcagag aagggtcttt tgatttatga agatgttcgt
     1081 tttcagcccg atcgccgatg gacgttgtat ggtcgtgtga gcgtctttga cgcggattcc
     1141 aaatcagcgg tgtacgaata cgaaaacgat gtcgaaggcg catttgccaa tacgcgattt
     1201 tccggaaaag gattgcgctg gtatgtcttc acacagtatc acggattaaa tcgaatggat
     1261 atcggcttca aatactggca aatggcacgc agtgatattt cggggtacgg tcgtatgaca
     1321 cggcgggtta ccttatcggt agattacaaa ttataaaacg ccgtggcatc ggaaaaaacc
     1381 tttgctcttt ttgacggtac ggggtatatt gagccgctta tctgcattcg caatacacaa
     1441 gctaaaatga tgttataaca tatcgccgga ggaatttgga gtatggaaga aaaaacgttg
     1501 gtcggctatt ttacggatgg tggttttttc atgtggccga ttttgatatg ctttatcggc
     1561 ggccttgcca tttgtatcga acgccttata acgctgacgc gtgcggcgac caataccaaa
     1621 caattcctcg tcaaaatcaa acaatcgctg gatgaaggcg gtgtcgagcg tgcgacggaa
     1681 gtatgcgcga gccagcgcgg atccgtggcg ttgatttttc atgcgggtct tttacgcgct
     1741 cgtaaaggtg tggagcatgt cgaaaaagcg attgttaatg ccggcgctgt ggaaatggcg
     1801 tttctggagc gcggtatggt atggcttggt ttttttgtcg gcgcggcacc tcttttgggt
     1861 tttacaggta cggtccaagg tatgatcgaa gcgtttgatg cgatcgccaa agctaatgac
     1921 atttcaccta cgattgtggc cgacggtatc cgtacggcgc tatatacgac gttattcgga
     1981 cttatggtgg cgattctttt acaatttttc ttcaattatt ttaccgcgaa aatcaatcgt
     2041 atcgtggctg atatggaaga aagttcttcg gaactggtgg actacctgct cgaaaacgaa
     2101 gtacggtaat aataatttta taaaattatg tttgtcgaaa aacgacgcaa atatactcct
     2161 gaaatttcaa ccgcttcgat ggcggatatc gcttttcttt tgatcatttt ttttatcgtg
     2221 atcacaaagt ttggcaccga taaaggcatt gatctgacac tgcctccgga aggttccatt
     2281 aaagaaattc ccaaaaacaa tatcatgagc attacgatgg atgatgccgg tgcggttgtt
     2341 ctggatggga aagcggtaat tctggggcag gttcagtcgc tcgtaaaaac catcgccgag
     2401 gccaacgacc gtttggtgat taccgtaaaa acaacacgca aaacgccgta tcgcaatttt
     2461 attgccgtga tggatcaact caaagaagcc ggtgtccgta aaatttcagt tttggagcct
     2521 gatcgctgat atgaagtttc gacgtcgccc tatagaatct ttgccggtat cgagcgcttc
     2581 gatgtccgac attgtttttt cgcttctatt gttctttatg gtatctaccg tgatcaaaaa
     2641 atacagcggc ttgcctgtgc aagtcccggc tgcatttaat atcgaaaaat tatccacgaa
     2701 aacgcataca tcttacc
//
LOCUS       DAOQNQ010000382         2715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_20865_length_2715_cov_4.686466, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000382 DAOQNQ010000000
VERSION     DAOQNQ010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20865_length_2715_cov_4.686466"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..833)
                     /locus_tag="PK754_15240"
     CDS             complement(132..833)
                     /locus_tag="PK754_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10906.1"
                     /translation="MKRNHYWIGLVFALSIISCGGDSETSITRDGDILIIGDGATDLS
                     LDTILSANNLTFSTVPMDSGFYGDELNSENISTVILCDGEGYEFGMPEQGQQALLDFV
                     ANGGRLILMEWIAYEIDSDRYTVLDTIVPVLRSSGREGTETYTVQLFNELTEGLDTTF
                     TVSSGSNIGTARFGTVHIKGSSSGDALVTHTWGQGEVIQMACAGNYEGYNPFAEPNMK
                     KLILNAVMISSLPKK"
     gene            complement(909..1889)
                     /locus_tag="PK754_15245"
     CDS             complement(909..1889)
                     /locus_tag="PK754_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HNB10907.1"
                     /translation="MKPHNSISQFMDHHYRHFNSAAMMDASKAYIKHLENGGQMFVTL
                     AGAMSTAELGLSLAEMIRQDKVHAITCTGANLEEDVFNLVAHDHYVRVPHYRDLTPQD
                     EQKLLDRHLNRVTDTCIPEGEAMRRIESAVLEEWVKADKAGERFFPHEFMYKILLSGK
                     LEKYYQIDPKDSWMMAAAKKNLPMFVPGWEDATLGNMYAGHVISGDVKNVHTVRTGIE
                     YMMRLADWYTERSKEHSVGFFQIGGGIAGDFPICVVPMLHQDLQRTNVPLWGYFCQIS
                     DSTTSYGSYSGAVPNEKITWGKLGIDTPKFIVESDATIVAPLMFAYILGW"
     gene            2045..2365
                     /locus_tag="PK754_15250"
     CDS             2045..2365
                     /locus_tag="PK754_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNB10908.1"
                     /translation="MAQSKALEFKQEEILLAEFAKALAHPARIAILKELARRSECICG
                     DLVEVLPLAQSTVSQHLKELQKIGLIQGEIDGPKSCYCINKKNMDKYLSILNKFFNHI
                     ESCC"
     gene            2386..>2715
                     /locus_tag="PK754_15255"
     CDS             2386..>2715
                     /locus_tag="PK754_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019945315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite S-adenosylmethyltransferase"
                     /protein_id="HNB10909.1"
                     /translation="MVNDEKLKEVVREKYGQIALTTLDDNAGSCCGVGGCGTIDYNIM
                     NEDYSQLDGYNPDADLGLGCGIPTEFAQIRAGQTVVDLGSGAGNDAFVARKLVGPSGF
                     VIGVDMTP"
BASE COUNT          799 a          630 c          544 g          742 t
ORIGIN      
        1 ggatacaggc aacaacgaat gagcttcaca taactgggat ttaccaaata ataaaagcct
       61 ccgtatgttg acggaggctt ttttcatgca aataaatcat attaattgat caattaaata
      121 agaaatattg atcacttttt aggcagtgaa gaaatcatca cagcattgag aattaatttt
      181 ttcatgttag gctccgcgaa cggattatag ccttcataat tgccggcaca agccatttgg
      241 attacttcgc cttgtcccca cgtatgggtg accagcgcat ccccagaact gcttccttta
      301 atatgcacag ttccaaaacg tgccgttcca atattggatc cgctactaac ggtaaacgta
      361 gtatccaacc cctccgtcaa ttcgttaaac aattgcacgg tatatgtttc tgtgccttca
      421 cggccgcttg accttaaaac tggaacaatg gtatcaagca ctgtatagcg atcactatca
      481 atctcatagg cgatccattc cataaggatc agacgacctc cgttagcaac aaaatcaagc
      541 agagcctgtt gtccttgttc gggcatacca aactcgtatc cttcaccatc acacagaata
      601 accgttgaaa tattttcgga atttaattcg tcgccgtaaa atccggaatc catcggtaca
      661 gtagaaaatg tcaaattgtt agcagataaa atcgtatcca gagacaaatc cgttgcaccg
      721 tcaccgatga tcagtatatc tccgtcgcgt gtgatacttg tttcgctatc tccgccgcag
      781 gaaataattg ataatgcgaa aacaagccca atccaataat ggtttctttt catactcttc
      841 tcctctttta aataaatgag aaccgaaccc tcatcaaaac ttttcgcaga atgcaacttc
      901 gccgcaaatc accatcccaa aatatacgca aacatcagcg gtgccacaat cgtcgcatcg
      961 gattcgacga taaatttagg cgtgtctata ccgagttttc cccatgtgat tttttcgttg
     1021 ggcacagcgc cggagtatga accgtaactc gtcgtcgaat cgctgatctg acagaaatat
     1081 ccccacaggg gcacattggt acgttgcagg tcctgatgca gcatgggaac aacacagatc
     1141 ggaaaatcac cggcaatgcc gccgccgatt tgaaagaaac cgacggaatg ttctttggaa
     1201 cgctcggtgt accaatcggc cagtcgcatc atatattcga tccccgtgcg tacggtatga
     1261 acatttttta catcgccgct gatgacatga cccgcataca tattacccaa tgtagcatct
     1321 tcccaaccgg gaacgaacat aggcaaattt ttcttagccg cggccatcat ccagctatct
     1381 ttggggtcaa tctgataata tttttctaat tttccgctga ggagaatttt atacataaat
     1441 tcgtgaggga aaaaacgttc accggcttta tcggctttta cccactcttc caatacagct
     1501 gactcgatcc gccgcatggc ttcaccttca ggaatgcagg tgtccgtgac gcgattcaaa
     1561 tgacgatcaa gtaatttttg ttcgtcttgc ggtgtcagat cacgatagtg cggcacacga
     1621 acatagtgat catgagcaac aagattaaaa acatcttctt ccaaattggc acccgtgcat
     1681 gtgatcgcat gaactttatc ctgcctgatc atctccgcca aggatagccc gagttcagcc
     1741 gtactcatgg ccccagccaa cgtaacaaac atctgcccgc cgttttcgag atgtttgatg
     1801 tacgctttgg aggcatccat catagccgcc gaattaaaat ggcggtaatg atgatccata
     1861 aattgactaa tcgagttgtg tggtttcatt tttttatcct gtatgtaaat gtttatcaat
     1921 gatttccgtg cgcttaagat aaccatttat gaaaaaaata aaagtttttt ttaaatcctg
     1981 cttgacaaat gaaacatttt gaattattat atcgtatatt tacgataaac gataaagaaa
     2041 aattatggca caaagtaaag cgctcgaatt taaacaagaa gaaatcttat tggccgaatt
     2101 tgccaaagca ctcgcccatc cggcgcgaat tgcaattctc aaagaactgg cccggcgcag
     2161 cgaatgtata tgcggagacc tcgttgaggt gttaccgctc gcgcaatcca ccgtttccca
     2221 acatttaaag gaattacaaa aaatcggctt gattcaaggt gaaattgacg gaccgaaatc
     2281 ttgctactgt atcaacaaaa aaaacatgga caagtatcta agcattctta acaaattttt
     2341 taatcacatc gaatcgtgtt gttaacaaaa accaaaggag taactatggt aaatgatgaa
     2401 aaactaaaag aagttgttcg tgaaaaatac ggtcagatcg cactgacaac actggacgac
     2461 aatgccggtt cttgctgcgg cgtcggtggc tgtggcacta ttgattacaa tattatgaat
     2521 gaagattatt cacagcttga tggttacaat cccgatgccg atctcggtct aggttgcggt
     2581 attcctacgg aatttgctca aatccgtgca ggtcagaccg tcgtggattt gggttccggc
     2641 gcaggtaacg atgctttcgt tgcacgaaaa ttagtcggcc cgtccggttt tgtcatcggc
     2701 gtggatatga cgccc
//
LOCUS       DAOQNQ010000383         2698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_21116_length_2698_cov_6.620129, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000383 DAOQNQ010000000
VERSION     DAOQNQ010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21116_length_2698_cov_6.620129"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..126
                     /locus_tag="PK754_15260"
     CDS             <1..126
                     /locus_tag="PK754_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011057625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNB10910.1"
                     /translation="IPVTGRDLRNAIEAVLSGHRPSTEQRPSLGCNIKWKPGNEP"
     gene            192..437
                     /locus_tag="PK754_15265"
     CDS             192..437
                     /locus_tag="PK754_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10911.1"
                     /translation="MFDWFKKKKKLNPKPKQSTNFDSGTMYDPLSPTYVTAGLASGAI
                     EEETVDDRNIEDSSNSDDTPESSSDASDSGSDSGGSD"
     gene            527..1075
                     /locus_tag="PK754_15270"
     CDS             527..1075
                     /locus_tag="PK754_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011398549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB10912.1"
                     /translation="MAVRSKKAVSKKNVARKPAKKKQIKKKVHFIPKGFHTLTPYLSV
                     HDGKAAVEFYEKAFGAKERKGRMSDEKGKIMHTEIKIGVSHFMLSDEFPEYGNVSPKT
                     VGKSPVQLMIYVPNVDILFEKAIAAGATVVMAVEDQFYGDRSGRLLDPFGYQWHIATH
                     MENVSPKEMARRAAKLYGAQAS"
     gene            1091..1657
                     /locus_tag="PK754_15275"
     CDS             1091..1657
                     /locus_tag="PK754_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB10913.1"
                     /translation="MKYKSCTTDGELLQILDLQKRNLPDLLTNEERQSQGFVTVKHDF
                     ETLEAMNTPFAHSLAISDDRVVGYALTMERSMEARIPILQPMFTTLDTLSIQNRKMSD
                     EGYIVMGQICVEKAFRGKGIFRGLYSHMRSRLGPYFRYLITEISERNVRSLNAHSAVG
                     FQKLITYEAPDGERWVIVGWNWNQSNTK"
     gene            1670..2086
                     /locus_tag="PK754_15280"
     CDS             1670..2086
                     /locus_tag="PK754_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNB10914.1"
                     /translation="MAENKTKATKLSVQDFLKTIKDEQTVKDCNVLIKLMKKITGKPA
                     VMWGPSIIGFDSYHYKYESGREGDMCITGFSPRKGKISIYITSGFERYTAHLKKIGKC
                     KTSKACLYVKSLTDIDTQVLEEMIKDSIIFMKKKWK"
     gene            2283..>2698
                     /locus_tag="PK754_15285"
     CDS             2283..>2698
                     /locus_tag="PK754_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10915.1"
                     /translation="MKHVKTVILLLVFFSSFVFSHGGGKNVKLHVNPKWKQCSIQLDS
                     ALNQSAWRQFTREAGLVSYFRPLTDAKPMGKGNYEFSLLQWQSKFDDTDDAWNHTFVH
                     PDSTHWLKENDRLPFPGLTFRLGISEKMDLAAYWTSS"
BASE COUNT          873 a          521 c          574 g          730 t
ORIGIN      
        1 attccggtta ctggaaggga tctacgcaat gcgatagagg ccgtgctttc cggccatcgg
       61 ccttcaacag agcagcgacc gagcctcggc tgcaatatca aatggaaacc cggtaatgaa
      121 ccctaatgcg gtatgcatat ccacgtagtg gtattacata tttaaagtaa cataaaacag
      181 aaggcgcgat catgtttgat tggtttaaga agaagaaaaa actcaaccca aagcccaaac
      241 aaagtacaaa ttttgactcc ggtacgatgt acgatccatt atcgccgaca tatgttactg
      301 caggtttagc gtcgggagct attgaggagg aaactgtaga tgatcgaaat attgaagact
      361 cttcgaattc agatgataca ccggaaagca gttctgacgc atccgatagc ggtagtgatt
      421 ccggagggtc cgactaattt ttatgagtaa atgaaacaaa ttgacttctg aagcagtatc
      481 ttatcttcta atcatcctta ttcaacccta aagaaggatt acaactatgg ctgttcgttc
      541 taaaaaagct gtttccaaaa aaaacgtagc ccgtaaaccc gcaaagaaaa aacaaatcaa
      601 aaagaaagtt cattttattc ccaaaggttt tcacacatta acaccttacc tcagtgtaca
      661 cgacggtaaa gcggcggttg agttttatga aaaagcgttt ggcgccaaag aaagaaaagg
      721 tcgcatgtcc gatgaaaaag gtaaaatcat gcatacggag attaaaatcg gcgtatcaca
      781 ctttatgctt tctgatgaat tcccggaata cggcaatgta agccctaaaa ccgtagggaa
      841 gtcgcctgta cagctcatga tttacgtacc caatgttgac atcctattcg aaaaagctat
      901 tgccgcagga gccaccgtag ttatggccgt ggaagatcag ttttacggtg accgctcggg
      961 acgccttttg gatccgttcg gttaccaatg gcatattgcg acccatatgg aaaacgtttc
     1021 tccaaaagaa atggcccgcc gcgccgcaaa gctatatggt gcacaggcct cataaagcat
     1081 tttttaaaat atgaaatata agtcatgcac aacggatggc gaattgcttc aaattctgga
     1141 tttgcaaaag cgcaatcttc cggatttgtt aacaaatgag gagcgccaaa gccagggatt
     1201 tgttacggta aaacatgatt ttgagacact tgaagctatg aatacgccgt ttgcacatag
     1261 tttggccatc tccgatgatc gtgttgtggg ttatgctctg acgatggagc gttcgatgga
     1321 agcacgaata cccatccttc aacctatgtt cactacgttg gatacattat ccatccaaaa
     1381 cagaaaaatg tccgatgaag gatatatcgt catggggcag atctgcgtag aaaaagcttt
     1441 tcgtggaaaa ggtatttttc gtgggctcta ttcgcatatg cgttcacgcc ttggacctta
     1501 ttttagatat ctcatcacag agatatcaga gcgtaatgta cgctcgctta atgcccactc
     1561 cgcagtcggc tttcaaaaac taatcacgta tgaagcaccc gatggcgaaa ggtgggtgat
     1621 agtcggatgg aattggaatc aatcaaacac aaagtaagga tacaagtgca tggccgaaaa
     1681 caaaaccaaa gctacaaagc tgagtgtcca ggatttttta aagacgatta aagatgagca
     1741 gaccgtcaag gattgtaatg tactgatcaa actaatgaaa aaaataacag gcaaacctgc
     1801 tgttatgtgg gggccatcaa tcatcggttt tgacagttat cattacaaat atgaaagtgg
     1861 acgtgaaggt gatatgtgta ttactggttt ttcaccccgc aaaggtaaaa tatccattta
     1921 cattacatca ggttttgaac gttatactgc tcatctaaaa aagatcggta aatgtaaaac
     1981 gagtaaagca tgcctttatg taaagtcgct tacagatatt gatacccaag ttcttgaaga
     2041 aatgataaag gattcgatca ttttcatgaa aaagaagtgg aaataagagt ttaagataac
     2101 tcaagaatca aaaaaacaga atatcacaag ctgttattta ttataaaatt gaccatatca
     2161 ccctataaga tgacacctgt ttcatgtatc atttaacacg ctgaagcgat catatctatc
     2221 ctttttatta aattttaact gtttgtcagt aaaaagttat caattaaaaa gaagggtaga
     2281 atatgaaaca tgtaaaaaca gttattctgt tattggtttt ctttagctcg tttgttttta
     2341 gtcacggcgg cggaaagaat gtaaaacttc acgtcaatcc aaagtggaaa cagtgctcga
     2401 tccaacttga ttcggcttta aaccagtcag catggcgcca gtttacacga gaagcaggac
     2461 ttgtatcata ttttcgacct ttgacggatg ctaagccgat gggtaaagga aactatgaat
     2521 tttctttgct tcagtggcag tccaagtttg atgatacgga tgacgcatgg aatcatactt
     2581 ttgttcatcc ggattccacg cactggctta aagaaaatga taggttgcct ttcccgggat
     2641 tgacttttcg attgggtata tcagaaaaaa tggacttagc ggcttattgg acatcatc
//
LOCUS       DAOQNQ010000384         2692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_21197_length_2692_cov_5.485400, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000384 DAOQNQ010000000
VERSION     DAOQNQ010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21197_length_2692_cov_5.485400"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2692
                     /locus_tag="PK754_15290"
     CDS             <1..>2692
                     /locus_tag="PK754_15290"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNB10916.1"
                     /translation="PLITGFADASGGYLGDVRLALRNYFGYNITATQDSAGNTSGFAV
                     NTWPFGLTDPTLVANNNNSGTGSLRFAINYANSHAGPDTIRFDVPMVGQTIFPATMLP
                     DITDDSTVIDGDIDMNGLPDITLNGAPFNVPIGLTINSNYNEIRNLNITGFGSMASGI
                     DINGGAYNLIVGCRIYNNNDGVNVFNGAHHNRIGDGTIAGRNYIYNNTDAFTGFGILI
                     EGFGTDSNIVYGNFIGTADGLTESGNSSDGVMVRNQARYNRIGGVGGSYNVISGNGRN
                     GIRIESDSNTVTGNFIGLDSIGTGALGNQGYGIHLLRAHANTIGGTAVGSGNVIVDNS
                     QANLNGAGVYLDSATYNEILGNYLGVDVYGNSSIANQSGGGGGSSITLDNQSNHNIIG
                     DGTAGGRNVMGAGGSGADMASHVLITNNSNFNLIAGNYIGVGSDGATALGIGSGVYIS
                     GSIKNQIGLPAPAEANIISGNPDGGIVLSDADSTLVLQNYIGVNASGNAAVGNSFGVR
                     LNNGTTHANIGNATVSGRNIISGNSNFGISIEDVGTDSNLVRSNLIGLDLAGTTAVPN
                     SNAGISIQDGVDNEIGDGSVGGRNVISGNSGHGIYNFSRTRIAGNFVGLDISGNDAIG
                     NTGYGIYTVSDSTQISGNVISGNSFTGIQVVYGRKAQIQGNAIGLDATGTIQRANGTA
                     GVVLFNHSDALVESNTITGNTGDGIRIEELSFGNTIFSNNIGMKDGPIGNSQNGIVLI
                     DNGSSYPSNNIIDSNYIAYNGNFGITIQGTQTDSNAAARNYIYKNIGGSIDISGGAQQ
                     GINPPTITLGTYGGTVAGTANSNALVYVYADSADQGEYFLDTVRADGSGNWTVSVTPV
                     SGTRYTAIQEYNAQTSEFSAPFIDPLLVINTNDAGAGSLRD"
BASE COUNT          727 a          551 c          668 g          746 t
ORIGIN      
        1 cctttgatta cagggtttgc cgatgcatcg ggcggttatc ttggtgatgt tcgcctggcc
       61 ttacgtaatt attttggcta caacataacg gctacacaag atagcgccgg taatacgtcc
      121 ggatttgccg ttaatacatg gccgtttggc ttaacggatc cgacacttgt tgccaataac
      181 aataattccg gtacgggaag tttgcgtttt gcaatcaact atgccaattc gcatgccgga
      241 cctgatacca ttcgttttga tgtaccgatg gtgggacaaa cgatttttcc tgccacaatg
      301 ctgcccgata tcacagatga ttcgacagtg attgacggtg atattgatat gaatggttta
      361 ccggatatca cattaaacgg ggccccgttt aatgtaccta tcggtttgac gattaattcg
      421 aattacaacg aaattcgtaa tttgaatatt accggttttg gttcgatggc gagcggtatt
      481 gatattaacg gaggcgcata caatctcatc gtcggttgcc gaatatataa taataatgat
      541 ggtgtcaatg ttttcaatgg tgcgcaccat aaccgtatcg gtgacggtac cattgcaggt
      601 cgaaattata tttataacaa tacggatgct tttacgggat tcggtattct gatcgaaggg
      661 tttggtacgg attcgaatat cgtctatggt aattttatcg gtacggccga cggtcttaca
      721 gaatcaggca acagttcgga cggtgtgatg gtacgcaatc aagcacgata caatcgtatt
      781 ggcggtgtcg ggggatcata taacgttatt tcaggaaacg gacgcaatgg tattcgtata
      841 gaatccgaca gcaataccgt gaccggtaac tttatcggtt tggattctat cggaaccggg
      901 gcgctcggca atcaaggata cggaattcac ttattacgtg ctcacgccaa cacgatcggc
      961 ggtacagcgg ttggctccgg aaatgtgatt gtcgataatt cgcaagctaa tcttaatggc
     1021 gcaggcgttt atctcgatag tgcaacttat aatgaaatat tgggcaatta tctcggcgtg
     1081 gatgtgtatg gcaattcatc catagccaac caatccggcg gcggtggcgg ttcaagtatt
     1141 acgctggata atcaatccaa tcataatatc atcggagacg gtacagcagg cggacgcaat
     1201 gtgatgggtg ccggtggtag cggcgctgat atggcttcgc atgtacttat tacaaacaac
     1261 tccaatttta atctcatcgc cggtaactat atcggtgtcg gttcagacgg cgccacggca
     1321 ttaggtattg gaagcggtgt ttacataagc ggaagtataa aaaatcaaat tggtttgccg
     1381 gctccggctg aggccaatat catatcgggt aaccctgacg gtggtatcgt gttgagcgac
     1441 gccgattcta cactagtatt gcaaaactat attggtgtca acgcgtctgg aaacgcagcc
     1501 gtaggcaaca gctttggtgt ccggttaaac aacggaacaa cgcacgctaa tatcgggaac
     1561 gcaacggtct caggtagaaa cattatttcc ggcaatagca atttcggtat atccatagaa
     1621 gatgtcggca cggatagtaa cttagtacgc agcaatttga tcgggcttga tctcgcaggt
     1681 acgacagccg ttccgaattc taacgccggt atttccattc aggatggagt ggataatgaa
     1741 ataggtgacg gttctgtcgg agggcgcaat gttatttccg gtaattcagg ccatggtatt
     1801 tataatttta gtcgaacacg tatcgcaggc aatttcgttg gtcttgacat cagtggcaat
     1861 gatgctatcg gaaatacagg atatggtatt tatactgtat cggacagtac gcaaatatcc
     1921 ggtaatgtta tttcaggaaa ttcatttacc ggaattcagg tggtgtatgg tcgtaaagca
     1981 caaattcaag ggaatgcaat cggtcttgac gcgacaggca cgattcaacg tgccaatggt
     2041 acagccggtg ttgtactttt taatcattcc gatgctttag tcgaaagtaa tacaataacc
     2101 ggaaataccg gtgatggcat tcgtatagaa gagttgtctt ttggtaatac tatctttagt
     2161 aataatatag gtatgaaaga tggtcccata ggcaattcac aaaacggtat cgtgcttatt
     2221 gataatgggt cttcttatcc gtctaataat attattgatt ctaattatat cgcatacaac
     2281 ggaaacttcg gaattactat tcaaggtaca caaaccgaca gcaatgcagc ggcacgcaat
     2341 tacatctata aaaacatcgg cggcagtatt gacatcagcg gtggtgcgca gcagggtata
     2401 aatcctccta cgattacatt ggggacgtat ggcggaacgg ttgccggtac ggcaaacagt
     2461 aatgcgctag tgtacgttta cgccgattct gccgatcaag gtgaatattt cttagataca
     2521 gttcgtgcgg atggctccgg taactggacc gtcagcgtaa cacctgtcag tggcacacgc
     2581 tataccgcca ttcaggaata taatgcgcag acgtcggaat tttccgcgcc gtttatcgat
     2641 ccgctattag tcatcaatac caacgatgcc ggtgccggtt cactgcgtga tg
//
LOCUS       DAOQNQ010000385         2681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_21377_length_2681_cov_4.873191, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000385 DAOQNQ010000000
VERSION     DAOQNQ010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21377_length_2681_cov_4.873191"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PK754_15295"
     CDS             complement(<1..306)
                     /locus_tag="PK754_15295"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNB10917.1"
                     /translation="MDTVKHLGELAIGSRLRRLSDRLLANVNRIYQEHGINFETRWFL
                     MFYQLHLKSPMSITEIAAATGFTHPAIIQLSDELIKVGLIASVTDTTDKRRRLISLSA
                     "
     gene            complement(388..1347)
                     /locus_tag="PK754_15300"
     CDS             complement(388..1347)
                     /locus_tag="PK754_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007200831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HNB10918.1"
                     /translation="MMPYRILLYYKFTEISDYEKFAAEHLTLCQSLGLLGRIIVAPEG
                     INGTVSGTVEQTDIYQKTILSDPRFSDMVIKSDPSDKHAFRRLSVKARTEIVTLGPDN
                     VNPTEKTGHYLEPPDFYAAMKEPDVVIIDARNDYEYDMGHFRGAIRPDVSNFKEFPAW
                     VRQNLSSYRDKKVLTYCTGGIRCEKFTGLLINEGFKDVYQLHGGIVMYGKHPDTKGRD
                     FEGKMYVFDERIGVEVNHTETASIITRCLYCETVTDHFVNCAHLDCHKSFYICAACDR
                     ANQRSCSEACKNADHHEYRVLGDALFEAPLTPRGLFKSILPTS"
     gene            complement(1355..1831)
                     /locus_tag="PK754_15305"
     CDS             complement(1355..1831)
                     /locus_tag="PK754_15305"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013943377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB10919.1"
                     /translation="MPDTIIVLETTQGTIEVKLFPDIAPKASENFEQLVEKGYYDGII
                     FHRIIKDFMIQGGDPTGTGRGGQSVWGMPFDDEFSFKVQFDKPGILAMANAGPRTNGS
                     QFFITTVPTPWLNNRHSIFGEVISGYDVVKKLEGVKTNPMDKPLQDQKIIKAYKKA"
     gene            complement(1890..>2681)
                     /locus_tag="PK754_15310"
     CDS             complement(1890..>2681)
                     /locus_tag="PK754_15310"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNB10920.1"
                     /translation="IALPALTLLHVHTLKISTDIWLPASMAWFFFAISAVTFYGLGHL
                     LHWDRSTTGCMMLVGGLGNTSFLGVPMIETFFGKEGIPIGLVVDQLGSFLVISTLGIW
                     TVNYYQGQSVSLKMLIKRIIVFPPFIALMLAVALIPYEFPAPLTYTLDRLGQTLAPLA
                     LFSVGFQIHFSKINSLRIPLVLGLSFKLLIAPAMLFILYSTLTADRYLLFDITIFEAA
                     MPPMITAAILATEHDLRPELASLMVGIGIIVSMFTLPFWWVLLTL"
BASE COUNT          788 a          561 c          604 g          728 t
ORIGIN      
        1 ggccgacaaa gaaatcagac ggcgacgctt atctgtggta tcggtaacgg atgcgatcaa
       61 tccaacctta attaattcat ccgatagttg gataatagcc ggatgcgtaa aaccggtagc
      121 tgccgcaatc tctgtaatag acatgggcga cttgagatgc aattggtaaa acatcaaaaa
      181 ccaacgcgtt tcaaaattta tcccgtgttc ctgatagata cgatttacat tggctaaaag
      241 tcggtcactg agtcggcgaa gtctgcttcc gatggcaagc tcacccaagt gttttactgt
      301 atccataatt gcctctattt atacgtaata cgttacgtat aacctataga atataggatt
      361 aaaatgcaac gaatttgggc ggcaaaatca ggaggttggt aatatggatt taaataagcc
      421 gcgcggtgtt aagggggctt cgaaaagcgc gtcgccgagt acacggtatt catgatgatc
      481 tgcatttttg caggcttcgg agcaactgcg ttggttggcg cggtcacaag cggcacatat
      541 ataaaaactt ttgtggcagt ccaagtgtgc acagttgaca aagtggtctg tcacggtttc
      601 acagtataaa caccgagtaa tgatggacgc tgtttctgta tggttgactt cgacgccgat
      661 gcgttcatca aacacataca ttttgccttc aaagtctctg cccttcgtat caggatgctt
      721 accgtacatt acaatgccgc catgcagttg atagacgtct ttaaatcctt cattgatcag
      781 caaaccggta aatttttcac agcgaatgcc gccagtacaa tacgtaagaa cttttttatc
      841 acgataagaa ctgaggtttt ggcgaaccca cgcgggaaat tctttgaagt tacttacatc
      901 aggtcgtatt gcccctctaa agtgacccat gtcgtactcg tagtcattgc gtgcatcgat
      961 gatcacgaca tccggttctt tcattgcggc ataaaaatca ggtggttcca aataatgacc
     1021 cgttttttcg gtcggattga cattgtccgg gcctaatgta acaatttctg tacgtgcttt
     1081 gactgaaagg cggcgaaagg cgtgtttatc ggatggatcg gacttaataa ccatatccga
     1141 aaaacgcgga tccgaaagta ttgttttttg gtaaatgtcg gtttgttcta ccgtacctga
     1201 aaccgtaccg tttatacctt ccggagcaac gatgatccgc cccaaaaggc cgagcgattg
     1261 gcataatgta aggtgctcgg ccgcaaattt ttcgtaatcc gatatttcag taaatttata
     1321 atataaaagt atgcgatagg gcatcatgtg ccgatcaagc tttcttatag gctttgatga
     1381 ttttttgatc ttgtaatggt ttatccatcg gattagtttt aaccccttcc aatttcttaa
     1441 ccacatcata cccgctaatc acttcaccga aaatcgaatg acggttattg agccacggcg
     1501 tgggaactgt cgtaataaag aattggcttc cgttggtgcg cgggccggca ttagccatgg
     1561 caagaatacc cggtttatca aattgaacct tgaaactgaa ttcgtcatca aaaggcattc
     1621 cccacacgga ttggccgccg cgccctgtac ctgtaggatc accgccttgt atcataaaat
     1681 ctttaatgat gcgatgaaaa ataataccgt cataataacc cttttcgaca agctgctcaa
     1741 aattttctga agctttggga gcaatgtcag gaaataattt tacttcgatt gtaccttgcg
     1801 tggtttctaa aacgataatt gtgtccggca tagaatctcc ttaattttgg tcgcaatata
     1861 ataaaaagtt ttttttttac aagcgggatt tagagagtaa gaagtaccca ccaaaacggt
     1921 aacgtaaaca tggatacgat gatacctata cctaccatca gggaggccag ttcaggtcgc
     1981 agatcgtgct ctgtcgccaa aatagccgcc gtgatcatag gcggcatcgc agcttcaaaa
     2041 atcgtaatgt caaaaagcaa atagcgatcg gccgttagcg tgctatacaa aataaaaagc
     2101 atagcgggtg cgatgagcaa tttgaagctc aaacctaaaa caagtggtat gcgaagcgaa
     2161 tttattttag aaaaatgaat ttgaaaacct acagagaaca gcgcaagcgg tgctaatgtt
     2221 tgcccaagcc gatctagcgt ataggtcaag ggtgcgggaa attcataagg tatgagtgct
     2281 acagcaagca ttagcgctat aaatggtgga aaaacgatga tgcgtttgat taacattttc
     2341 aaagagacac tttggccttg ataataattg actgtccaga tccccaaggt tgatattaca
     2401 agaaatgatc cgagttggtc gacgacaagc ccgatcggaa tgccttcttt tccgaaaaag
     2461 gtttcaatca tcggtacacc aagaaaagag gtatttccga ggccgccgac aagcatcata
     2521 cagccggtag tgcttctatc ccaatgaagt agatggccga gcccataaaa agttacagca
     2581 gaaattgcaa agaaaaacca tgccatgctg gcggggagcc atatatcggt acttattttt
     2641 aacgtatgga catgcagtaa tgttaaggcg ggtagggcga t
//
LOCUS       DAOQNQ010000386         2677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_21432_length_2677_cov_6.061785, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000386 DAOQNQ010000000
VERSION     DAOQNQ010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21432_length_2677_cov_6.061785"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PK754_15315"
     CDS             complement(<1..1171)
                     /locus_tag="PK754_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10921.1"
                     /translation="MPDKLQPLALEDNAFMLSSFLSRFTFPLLFIFAAAIEFCMVRIS
                     GITSLRNDFESLYWHFGIAFGIYLLTVLYVRFYWGKTFFSPEWIIGTAIVFRLTLLFS
                     DPVLSDDIYRYIWDGRVSNHAVNPYAYAPESGNLYGIRDSVIYPQINHKELQTIYPPA
                     AQVLFRITSWISESVLAMKLMVLLFDIGIIFLIMALLRHFELPLGWIAVYAWNPLVII
                     EFSGNGHMDAIGIFFMLLAFWFSLREKTILSALPLALSFCTKYLTLPLLPFMEDIRSR
                     WKNVLAITAIFAGTVLLLYTGYSDVDAIKFGSLKIFSETWEFNASLYTVVENFFKTRL
                     DSSLTTVWGISTDNPARLAARLTIGISLTLFIMILYGINFTKSYAVQTKRRIYYAF"
     gene            1211..>2677
                     /locus_tag="PK754_15320"
     CDS             1211..>2677
                     /locus_tag="PK754_15320"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HNB10922.1"
                     /translation="MISNHHSIARWVMEARSVLVSARIMEIFCQQKNLLQIGLVTPSR
                     QSLVLEIYCDRQPYIVWRSSYSRKNKNSMDLFPMMIGLHIRDIVMDNTDRRVILSFEN
                     GCRLVAQFYGSANVWATDASGVITGSFKNERAVHESSMKIESGGADMRRYTQDAVDVF
                     ESVALFREALSGGFGHLNKILTLEFLHAVSLSDITTAREAWRNFINAIYDGAPRIYTD
                     HGDLKYFSLLPLTHHLGRNPHWKEELFTSVNDAFLAYVQLRFFAERLQKKRDDLLRAS
                     RQRIKKNEHLIMALQEDTEKAATYAAYEQKAHLLNIHLRDVHRGMDKIVVTDVYDEAQ
                     NEIEIKLDPILSPQANVEKYFAQSKKLKQSVVKIAERILTIGKENDTLRQIEAQFADA
                     HAIERKALEKKYDEFVRNGWIKKTESSKPTRAADQEMPVFREFRVHGNWRVFVGQNDI
                     KNDALTFRFAKKDDLWFHARGVAGSHVVLKRDGRSDNPG"
BASE COUNT          829 a          562 c          641 g          645 t
ORIGIN      
        1 aaaacgcata atatatacgg cgttttgtct gtaccgcata cgatttggta aaattgatcc
       61 cgtacaatat cataataaat aaagtgaggc ttattcctat cgttaggcgc gccgcaagcc
      121 gtgcggggtt gtccgtagaa ataccccaga ctgtcgtgag ggatgaatcc aagcgtgttt
      181 taaaaaaatt ttccacgacg gtgtataacg atgcattgaa ttcccatgtt tcagaaaaaa
      241 tcttaagcga accaaactta atcgcatcca catctgaata accggtatat aataataata
      301 ccgtacctgc aaagatcgcc gttatggcta atacattttt ccaccgcgaa cgtatatctt
      361 ccataaatgg gagcaagggc agtgtgagat atttggtgca gaaggacaaa gctaacggca
      421 gagcggataa aatcgttttt tcgcgcaggg aaaaccaaaa cgcaaggagc ataaaaaata
      481 taccgatggc gtccatgtgc ccgttgcctg aaaattcaat gatcactagg ggattccatg
      541 catataccgc gatccaaccc aacggtaact cgaagtgacg gagcagcgcc atgatgagaa
      601 aaataatgcc gatatcaaac aacaaaacca tcagcttcat tgcaagaacg gactcggata
      661 tccaggatgt aatgcgaaat aaaacctgtg ccgccggagg ataaatggtt tgtaattctt
      721 tatgattgat ttgggggtaa atcacactat cgcgaatgcc atacagattt cccgactccg
      781 gcgcataagc gtaagggtta acggcgtgat tagatacacg gccgtcccaa atatagcggt
      841 atatatcgtc cgacaaaacc ggatcggaaa atagcaatgt caatcgaaac acgatcgccg
      901 ttccaataat ccactccggt gaaaaaaaag ttttacccca ataaaagcgc acatacaaca
      961 ccgtcagcag gtaaattccg aatgcgatgc cgaaatgcca ataaagcgac tcgaagtcat
     1021 tgcgtaatga agtgatgccc gaaatgcgaa ccatgcaaaa ttcgatcgcg gcggcaaaaa
     1081 taaaaagtaa cggaaaagta aaacgggata aaaacgagga taacataaag gcgttgtctt
     1141 ctaaggcaag aggttgtaat ttgtcgggca atttaagaaa cagactccga aaggcaaata
     1201 gtaaattccc atgatcagca atcaccacag catcgcacgt tgggtcatgg aagcacgctc
     1261 cgtattggta tccgcgcgta tcatggagat tttttgtcag caaaaaaatc ttttacagat
     1321 cggattggtt acaccttccc gtcagtcatt ggttttggaa atatactgcg accgtcaacc
     1381 gtatatcgtt tggcgttcta gttattcgcg caaaaacaaa aattcgatgg atctttttcc
     1441 aatgatgatt ggtcttcaca tccgcgacat tgtcatggac aataccgacc gacgggtgat
     1501 tctatccttt gaaaatggtt gtcgtttggt agcacaattc tatggatcgg cgaatgtgtg
     1561 ggcgacggat gcatcgggtg taataaccgg atcgtttaaa aatgagcgcg cagtgcacga
     1621 gtcgtccatg aaaatagagt cgggtggggc ggatatgcgg cggtacacgc aggatgccgt
     1681 ggatgtgttt gaaagtgtgg cgcttttccg tgaagcgtta tccggcggat ttgggcattt
     1741 gaataaaatt ctgacgcttg agtttttgca cgccgtatca ttaagcgata taacaacggc
     1801 acgcgaagca tggcgaaact ttatcaatgc gatctatgac ggtgcaccgc gtatttatac
     1861 cgaccatggc gatttgaagt atttttcatt gttgccgctg acgcatcacc tggggcgaaa
     1921 cccgcattgg aaagaagaat tatttacgtc agttaatgat gcgttcttag cctatgtcca
     1981 gttgcgcttc tttgctgaac gtcttcaaaa aaaacgagat gatcttctgc gcgcgagccg
     2041 ccagcggatt aagaaaaacg aacatttgat catggcgctg caagaagata ccgaaaaggc
     2101 tgcgacgtat gcggcgtacg aacaaaaagc gcatcttttg aatattcatt tgcgtgacgt
     2161 gcatcgggga atggataaaa tagtcgttac cgatgtgtat gatgaggcgc aaaatgaaat
     2221 cgaaataaag ctcgatccga ttttatcgcc gcaagccaat gttgaaaaat actttgcgca
     2281 atccaaaaaa ctcaaacagt ctgtggtgaa aatcgccgaa cgtatcctca cgatcgggaa
     2341 agaaaatgat accttacggc aaatcgaagc gcaatttgcc gatgcgcatg cgatagaacg
     2401 aaaagcgtta gaaaaaaaat atgatgaatt tgtacgtaac ggttggatca aaaaaacaga
     2461 atcctccaaa ccgacgcgcg ccgcggatca ggaaatgccc gtttttcgcg aatttcgggt
     2521 gcatggcaac tggcgggttt ttgtcggaca gaatgatatt aaaaacgacg cgctgacgtt
     2581 tcgttttgca aaaaaagatg acttatggtt tcacgcgcgg ggcgtggcgg gttcgcatgt
     2641 cgtactcaaa cgcgacggac gctcggataa tccgggc
//
LOCUS       DAOQNQ010000387         2666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_21603_length_2666_cov_5.139027, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000387 DAOQNQ010000000
VERSION     DAOQNQ010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21603_length_2666_cov_5.139027"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="PK754_15325"
     CDS             complement(<1..167)
                     /locus_tag="PK754_15325"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNB10923.1"
                     /translation="MSSPLIGLTGGIGCGKTEVARIFERLGAIVIDADRLGRHVVESD
                     AAVRSEIRRAFG"
     gene            317..1342
                     /locus_tag="PK754_15330"
     CDS             317..1342
                     /locus_tag="PK754_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HNB10924.1"
                     /translation="MIDLRSDTVTQPSPAMRDAMASAKVGDDVFGEDPTVNALQEAVA
                     HMLGHEAGLFVPSGTMGNQLAIKTHTRPANEIIIEADAHIFKYECGASSMISGVMTKT
                     LHGQRGILTASQIAAAINGPDIHNAPTGMIALENTHNRAGGTIYPIETVKQISDLAHQ
                     RHIPLHLDGARLMNACVASHIKPQTYGVLFDSVSLCLSKGLGAPVGSVLVGNKSFIAQ
                     ARYYRKALGGGMRQAGILAAAGLYALQHHVNDLADDHRKAQILAETLSALPPFEIDVT
                     TVQTNIVIFDVVDRALDAQRVVDHLGSQGILAIPFGERKVRFVTHRDLTFEQIDEAVS
                     ILRKQYQ"
     gene            1587..2240
                     /gene="deoC"
                     /locus_tag="PK754_15335"
     CDS             1587..2240
                     /gene="deoC"
                     /locus_tag="PK754_15335"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNB10925.1"
                     /translation="MIDHTLLKPEATAKEIDQLCEEAAKYCFASVCINPSWVKRAAQK
                     LARSGVKVCTVVGFPLGATSTQAKALETEIAVSHGAQEVDMVINVGWLKSGEYELVKK
                     DIAAVVRHAGRQVPVKVILETCLLTNEEKIKACVLSKEAGAAYVKTSTGFNKGGATAA
                     DIALMRHVVGQDMGVKASGGVRTYEDALQMVQSGASRIGASASVKIVTGKSGEAAGY"
     gene            2286..>2666
                     /locus_tag="PK754_15340"
     CDS             2286..>2666
                     /locus_tag="PK754_15340"
                     /inference="COORDINATES: protein motif:HMM:NF012536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNB10926.1"
                     /translation="MPNRFKTIERHIIDMERLHPEASGELTGLLSDIALAGKIISSEV
                     NRAGLVNLLGLAGGENVHGEAVKKLDMFSHDMLVKIISGGRGEIFMLGSEEMEHPLSL
                     DDNYPDGKYIVNFDPLDGSSNIDAN"
BASE COUNT          719 a          662 c          629 g          656 t
ORIGIN      
        1 ccaaacgcgc gtcgtatttc cgatcgaacc gccgcatccg actccacgac atgacggcca
       61 agccgatcgg catcaatcac tattgcaccg agtctttcaa aaatacgcgc tacttccgtt
      121 ttgccgcatc cgataccgcc ggtgagaccg atcaaaggtg aactcattgc attcctgatt
      181 tgatgacatc cagacgtgcg gttaaaacta ccaaaacaca ggatgactat caatagccgt
      241 gatatgaaaa aatcttttgt ttaaattttt ttaaaatcgt attctctata aaaatttaac
      301 caattctcat ttatccgtga ttgatttacg cagcgatacg gtaacccagc cttcaccggc
      361 tatgcgggat gcgatggcct cggccaaggt tggcgacgac gtcttcggag aagatccgac
      421 agttaatgct ttacaggaag ccgtggcgca catgttgggc catgaagcgg gtttattcgt
      481 ccccagcggt acgatgggca accaattggc cattaaaaca cacacgcgcc ctgctaacga
      541 aatcatcatc gaggccgatg cgcatatttt taaatacgaa tgcggcgcct catccatgat
      601 ttccggcgtc atgaccaaaa ccttgcacgg acaacgcggt atccttaccg cttcgcagat
      661 cgcggcagct atcaatggtc cggatatcca caacgcgccc accggcatga tcgctttgga
      721 aaatacgcac aatcgcgcgg gtggtacgat ctatccgatt gaaactgtaa agcagatttc
      781 cgatctggca catcaacgcc atatcccttt acatttggac ggcgctcgtc tgatgaatgc
      841 ctgcgtagca agtcatatta aaccgcaaac ctacggtgtt ttattcgact ccgtgtctct
      901 ttgcctttct aaaggcttag gtgcgcccgt aggctctgtg ctggttggaa ataaatcttt
      961 tatcgcgcaa gcgcgctact atcgcaaagc attgggcggc ggtatgcgtc aggcgggtat
     1021 tctcgccgcg gccggtttat atgcattaca acatcatgtc aatgatctcg cagacgatca
     1081 ccgtaaggcc caaattttag ccgaaacatt atccgcattg ccgccttttg aaatagatgt
     1141 cacaacggtg caaaccaata tcgttatttt tgatgttgtg gatcgggctt tagatgcaca
     1201 acgtgtagta gatcatctcg gttcgcaagg tattttggcg attccattcg gggaacgcaa
     1261 agtacgtttt gttacccatc gcgatcttac gtttgaacaa atagatgaag ctgtttccat
     1321 acttcgtaaa caataccaat aaactcatga gcacagaaaa aatcattgct gaccaaattg
     1381 atcgcattct tgcggatatc aaaggggtta ccgccgtatc cgaaccttca tgcggttgcc
     1441 gcatcaataa gcccgcgacg ccaagcgaag cctgcgcatg caataccgca acggaagccg
     1501 aaatctataa caatttcgtc gaatgcggtg cgtgtcgcat ttccgctgcg ctcggcattg
     1561 atgccgaaaa agaacgtgtc gggcgaatga ttgatcatac gttgctcaag cccgaagcca
     1621 cagcgaaaga aattgaccaa ctctgtgaag aagcggccaa atactgtttt gcatcggtat
     1681 gtatcaatcc aagctgggtc aaacgcgccg cacaaaaatt ggcgcgtagc ggcgtcaaag
     1741 tgtgcaccgt cgtaggtttc cctttgggcg ctacaagcac acaggccaaa gctttagaaa
     1801 cggaaatcgc cgtatcgcac ggtgcacaag aagtggatat ggtgatcaat gtcggttggc
     1861 tcaaatccgg tgaatacgaa ttggtcaaaa aagatatcgc tgctgtagtt cgtcatgccg
     1921 gtcgccaagt gcccgtcaaa gtgattttag aaacatgcct tctcaccaat gaagaaaaaa
     1981 taaaagcctg cgtactttcc aaagaagccg gcgccgctta cgttaaaact tcgaccggtt
     2041 tcaacaaagg aggcgctacc gcggccgaca ttgctctgat gcggcatgtc gtcggacaag
     2101 acatgggcgt caaagcatcc ggcggcgttc gtacgtacga agatgcgctg caaatggttc
     2161 aatcgggcgc atcacgtatc ggcgcaagcg ccagcgtaaa aatcgttacc ggcaaatccg
     2221 gcgaagcagc cggttattga ttgatatgac gattaaaata tatcttaatt catcaggact
     2281 tcaacatgcc taatcgtttt aaaaccattg agcgacatat cattgacatg gaacgcctgc
     2341 accccgaggc atcgggtgaa ttgacaggtc tgctttccga cattgcgctc gcgggtaaaa
     2401 tcatttcatc agaagtcaat cgtgccggat tagtaaacct gctcggactg gctggcggcg
     2461 aaaatgttca cggcgaagcg gtaaaaaaat tagatatgtt ttctcacgac atgctggtca
     2521 aaattatttc gggcggacgg ggcgaaattt ttatgctggg ttccgaagag atggaacatc
     2581 ctctttcact tgatgataat tatcccgacg gcaagtatat cgtcaatttt gatccgcttg
     2641 acggttcatc caatattgat gccaat
//
LOCUS       DAOQNQ010000388         2639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_22034_length_2639_cov_5.606037, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000388 DAOQNQ010000000
VERSION     DAOQNQ010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22034_length_2639_cov_5.606037"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PK754_15345"
     CDS             complement(<1..514)
                     /locus_tag="PK754_15345"
                     /inference="COORDINATES: protein motif:HMM:NF014769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HNB10927.1"
                     /translation="MNQHLTIHPVLRTQIKHAIEEDLGFGDVTTLSTVPAEQIGRGYV
                     RAKSEGVLAGQLVFNEVFRQINPQIKLEWSAKDGDTLVKGQICLHFAGSMRSILIAER
                     TALNFIQRMSGIATLTRHFMERIKHTSAQILDTRKTTPLWRELEKYAVRCGGGSNHRI
                     GLYDMFLIKDN"
     gene            complement(511..936)
                     /gene="ybeY"
                     /locus_tag="PK754_15350"
     CDS             complement(511..936)
                     /gene="ybeY"
                     /locus_tag="PK754_15350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNB10928.1"
                     /translation="MITITNTHKHRRVPKQRIIALTKMILKKESPRNLEQFHFVFLDN
                     VTMRRFNKKFLNHDYPTDTITFDLSANRRASLEGECYISLDQAVMQAKEYGVTYTNEV
                     LRLCAHCVLHLLGYHDDTDQQRSRMIRRGDHYLTLLERQ"
     gene            complement(933..>2639)
                     /locus_tag="PK754_15355"
     CDS             complement(933..>2639)
                     /locus_tag="PK754_15355"
                     /inference="COORDINATES: protein motif:HMM:NF019318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNB10929.1"
                     /translation="NFKSPISPINVREGLKESTEFIKNVNDVDEARYHTQDLLKTYYS
                     DTKLINLSYEVLSRFIIPNILANRDETEARKREASANVPQTYGFVLSGDIIVNKNETI
                     TSKTHQQLQSLEATWAQRREAEGGVKWLLPFIGRSFLVFSLMFFLLGYIYIYRPDIFS
                     SIRKLSLLVSLILLEITFYYVFIHVLKFPAFVIPIVFSSVLLTTLFDVRIGLIGTISI
                     SFLIGAMQGNEYTTTIVLLFVGSVACITVVNFSRRSHIFSSVLWVSGAYIFIIATTSF
                     VKYTEFAEIFLHQLPYAIVSGMSSMLVAFGCLVLFEQVFDVSTTFTLLELSDSNHPLQ
                     QQLALKAPGTYHHSVIVANLSHAGAEATGANSLLARVGALYHDIGKIEMPEYFIENQI
                     GGVNKHDRLDPHMSAKILLSHIHSGLELAEKYRLPKIIRSYIPEHHGNLLMTFFYHRA
                     MENKKPDAVISEASFRYPGPRPKTKESGIIMLADGVEAATHAIKEPNAENIRNVVNEI
                     IERRLKSGELDECELTIGDLKRITEAFIPIILGIYHVRVEYPKADPLPIDGKNVTVGA
                     MS"
BASE COUNT          703 a          581 c          644 g          711 t
ORIGIN      
        1 gattatcttt gatcaaaaac atatcgtaca atccgatgcg atgattggaa cctccgccgc
       61 atcgtacggc gtatttttcg agttcccgcc aaagcggtgt agttttacgc gtatctaaaa
      121 tttgcgccga tgtgtgtttg attctctcca taaaatgccg tgtcaatgtg gcgataccgc
      181 tcattcgctg gataaaatta agcgccgtac gttccgcaat gaggatagac cgcatgctgc
      241 cggcaaaatg caggcagatt tgacctttta caagcgtatc accgtctttg gccgaccatt
      301 ccaatttgat ttgaggattg atttggcgaa agacttcatt gaaaacaagt tgcccggcta
      361 atacgccctc cgacttagca cgtacataac cgcgtccgat ttgttcggcc ggcacggttg
      421 agagtgtcgt tacatcgcca aagccgagat cttcttcgat agcgtgtttg atttgcgtac
      481 gtaaaacagg gtgtatcgta agatgttgat tcattgtctt tccagtaaag taagatagtg
      541 gtcgccgcgc cggatcatac gggaacgctg ttggtcggta tcgtcatgat agcccaagag
      601 gtgcaggacg cagtgcgcgc aaagccgtaa aacttcattg gtataagtga cgccgtactc
      661 tttagcttgc attacggcct ggtctaaact gatatagcat tcgccttcca gcgaggcgcg
      721 acggtttgca ctgagatcaa aagtaatcgt atcggtgggg taatcgtgat tcaaaaattt
      781 tttattgaaa cgtcgcattg taacattatc taaaaaaacg aaatgaaatt gttccaaatt
      841 ccttggcgat tcctttttca ggatcatttt cgtcaaagcg ataatacgtt gtttcggtac
      901 gcgccgatgt ttgtgggtat tggttattgt gatcatgaca tcgcgcctac ggtaacattt
      961 ttcccgtcaa tcggcaacgg atcggcttta ggatactcga cacgcacgtg gtagatgccc
     1021 aatatgatcg ggataaaagc ttcggtaatg cgtttgaggt caccgatggt cagttcgcat
     1081 tcgtcaagtt caccggactt gagacggcgt tcgatgattt cattgaccac attgcgaata
     1141 ttttcagcat tgggttcttt gattgcatgg gttgcggctt cgacgccgtc ggctaacatg
     1201 ataatgccgg attctttggt tttggggcgc gggcccggat aacgaaatga agcttcggat
     1261 atcacggcat ccggtttttt gttttccatc gcgcgatgat aaaaaaaggt catgagcaga
     1321 ttaccgtgat gttccgggat ataacttcgg atgattttcg gcaaacggta tttttcggct
     1381 aactccaggc cggaatgaat atgcgagagc agtatttttg cgctcatgtg cggatccagc
     1441 cgatcgtgtt tattcacacc gccgatctga ttttctatga aatattcggg catctcgatt
     1501 ttaccgatgt catgatacaa ggcgccgacg cgtgccagca acgaattcgc gccggttgct
     1561 tccgcgccgg catgggacag atttgcgacg attacactgt gatgataagt gcccggcgct
     1621 ttcagcgcca gttgttgttg caaagggtga ttggaatcag aaagttcgag taaagtaaat
     1681 gtcgtactga catcaaaaac ctgttcaaaa agtacaaggc atccgaatgc gaccaacatg
     1741 gacgacatgc cgctgacgat ggcgtaaggt aactgatgca aaaaaatttc agcaaattcg
     1801 gtatacttca caaagctggt cgtcgcaatg ataaaaatgt acgcgcccga cacccagaga
     1861 acggaactga aaatgtgact gcgccgactg aaattaacca cggtaatgca cgcaaccgaa
     1921 ccgacaaata acaatacaat ggtcgtcgtg tattcattgc cttgcatcgc tccgatcaaa
     1981 aaactgatcg agatcgtccc gatcagcccg atacgtacgt caaaaagtgt tgtcagcagg
     2041 acggaactaa atacgatggg aatcacaaag gccggaaatt tgagcacgtg aatgaacacg
     2101 taatagaaag tgatctccag caaaatcaag gacacgagca gtgataattt tcgaatactc
     2161 gaaaaaatat cgggcctgta tatgtaaata taacccagta aaaaaaacat cagtgaaaaa
     2221 actaaaaacg atcgtccgat aaacggcaat agccatttaa cgcctccttc cgcctcgcgg
     2281 cgctgcgccc acgtggcttc cagtgattgc aactgctggt gcgttttgga ggtgatggtt
     2341 tcgtttttat taacgatgat gtcgccgctt aaaacgaagc cataggtttg tgggacatta
     2401 gccgaagctt cacgtttacg cgcttccgtc tcgtcgcgat ttgccaaaat attgggaatg
     2461 atgaaacggg agagcacttc ataactcaga ttgatcagct tggtatccga ataataggtt
     2521 ttcagcaagt cttgcgtgtg gtagcgtgct tcatccacat cgttgacgtt tttgataaat
     2581 tccgtgcttt ccttaagacc ttcgcgtacg ttgatcgggg agatcgggct tttgaaatt
//
LOCUS       DAOQNQ010000389         2627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_22211_length_2627_cov_4.404355, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000389 DAOQNQ010000000
VERSION     DAOQNQ010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22211_length_2627_cov_4.404355"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1707
                     /locus_tag="PK754_15360"
     CDS             <1..1707
                     /locus_tag="PK754_15360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB10930.1"
                     /translation="PPWYRTWWAWLTYLMLFVVVVTGLVRWRSYQIMREKERLERIVE
                     ERTHLLSVQTQKLEALDAQKSRFFANISHEFRTPLTLLQGPLETMKEKPADDSTIQSM
                     LRNTRRLLRLVNQLLDLSKIEHRQMKLRAYRGTWDFIKPAVFSFESLANKKGLSFTLD
                     VSPDLPVMYRDRDVIEKILFNLLSNAIKFTNEGGVSVKVYVNEGALTIDVEDTGIGIS
                     ETDLVLIFDRFYQADHSGARAYPGTGIGLALAKELAHLHHGSLTARSRQGKGTVFSFT
                     IPIIEQSIPEDERTESETHSQESAPEWDDVTGPIQSATSADKDVDKNTLLIVEDNDEI
                     RQYIHGIVSNDYHVIEAADGEDGMAEALRRTPDIIISDVMMPRKDGITLSRELKSDRR
                     TSHIPLILLTAKADTEDKIEGLETGADDYLVKPFNEKELRTRVANLIQSRKLLREKYA
                     KEILIKDQDFEGETPDDLFLKELTQLIQSNLSDPEFGVEAISARLAMSRSQVYRKLVA
                     LTDTTPSELIRKLRLRKAKSLLESQAGNVTQVAYEVGYTSLSHFTKAFRDEFGINPSD
                     TI"
     gene            2234..>2627
                     /locus_tag="PK754_15365"
     CDS             2234..>2627
                     /locus_tag="PK754_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10931.1"
                     /translation="MYIIRFNRISLLVICSFILFGCEFSSKKTQNEIIPPAESEVTVN
                     GLMPDEQAIHDEGREIFFTKFTIDDGLGPFFAEQSCGLCHEGAGRGPGKVLRIGRTKN
                     GSFDPMAEFGGPTIQFRTGLKDRVPETVP"
BASE COUNT          763 a          504 c          611 g          749 t
ORIGIN      
        1 ccgccgtggt atcggacatg gtgggcatgg ttaacctatt tgatgttgtt tgtggttgta
       61 gtgacagggc ttgtgcgttg gcgttcctat cagatcatgc gtgaaaagga acggttagag
      121 cgtatcgtgg aggaacgtac gcatctattg tctgttcaga ctcaaaaatt agaagcgctt
      181 gatgcgcaga agtcgcgatt ttttgccaat atttctcacg aatttcgcac gcccctcact
      241 ttgttgcaag gcccgctgga gaccatgaag gaaaaaccgg ccgacgacag tacgatacaa
      301 tcaatgcttc gtaatacgcg acgactcctg cgcttagtta accaactttt agatttatct
      361 aaaatcgagc atcgtcagat gaagctgaga gcatatcgcg gtacgtggga ttttataaaa
      421 ccggctgtat tttcttttga atcgttagct aataaaaaag gtttgtcgtt tacgctggat
      481 gtatcgcctg atctaccggt gatgtatcgt gatcgcgatg tcatcgaaaa aatattattt
      541 aacctgcttt ccaatgcgat caagtttaca aacgaaggcg gtgtatctgt taaagtatat
      601 gtgaacgaag gcgctttgac aatagatgtc gaggatacgg gtatcggtat cagtgaaacc
      661 gatttagttt tgatttttga tcgtttttat caagccgatc attcgggtgc gcgcgcgtat
      721 cccggcaccg gtataggact cgcattggcc aaagagttag cgcatctcca tcatggatct
      781 ttgacagcgc gtagccggca agggaaggga acagttttta gctttacgat accgatcatc
      841 gaacaaagta tacccgaaga cgagcgtacc gaatcggaga cgcattccca agaaagtgcg
      901 cccgaatggg atgatgttac tggaccgatt caaagcgcca cgtctgccga taaggatgtc
      961 gataaaaata cgttgttgat tgtcgaagat aacgatgaaa tacgtcaata tatccacggt
     1021 atagtaagca atgattatca cgtcatagaa gccgcggacg gtgaagacgg tatggcggaa
     1081 gccttgcgaa gaacaccgga tattattatt tccgatgtga tgatgccccg aaaagacggt
     1141 attacattat cacgcgagtt gaaatccgat cgccgaacca gccatattcc gcttattcta
     1201 ttgacggcca aagccgatac cgaagataag attgaagggt tggagaccgg tgccgatgac
     1261 tatctggtaa aaccgtttaa cgaaaaagaa ttgcgaacgc gtgtagccaa tctgattcag
     1321 agccggaagc ttctgcgtga aaaatacgcg aaagaaattc ttatcaagga tcaggatttt
     1381 gaaggtgaaa caccggatga tctttttttg aaagaattaa cgcaactcat acagtcgaac
     1441 ctgtcggatc ctgaatttgg tgttgaggcg atcagtgcac gattggccat gagtcgctct
     1501 caggtttatc gcaaattagt cgcgcttacc gataccactc catccgaact catacgaaaa
     1561 ttgcgtttgc ggaaagccaa aagcttgctg gaatcacaag ccggtaatgt gacgcaggtc
     1621 gcttacgaag tcggttatac cagtttgtca cattttacca aagcttttcg cgatgaattt
     1681 ggtataaatc cctcggatac gatctgatct aaaagcttta aaaacaatct ccgcaacata
     1741 agttgcaaca aaaccgctag taattaacct actatcgtta gtacgcatct cgtcttatcc
     1801 ggaaattttc gaagaaaaaa tttgatcgag ttaatctgga ttaactcaga ttttgcgtga
     1861 atctagtata ttaattatta gtgatgagca tgtatttgat gtttgcgatc gaatgagtag
     1921 tcgggtttta caaaaagaat atttttttgt tgccagattc aatgtgatag ccttaagaga
     1981 atacttttca gtaagacttc gagttggata taatcccttg tttattttta cttacatggc
     2041 tgattgggct tattgatatt tccagagtgc agagatagta ttttctgagc tagtatatga
     2101 actttttctt ctttttagaa tttttttgat aaaagcaatg ccatgactat gaattacatt
     2161 ttaataaaaa ttagaagatt atatagaccg gcctaaatgt tcatgcaaca aaagttgatc
     2221 caaaggaaca agtatgtaca ttataagatt taacagaatt agtttattgg tgatctgtag
     2281 ttttatcctt ttcggatgcg agttttcatc caaaaaaaca caaaacgaaa tcatacctcc
     2341 tgctgaatcg gaagtaacag taaatgggtt aatgcctgac gagcaggcga ttcatgatga
     2401 ggggcgcgag atatttttta cgaaattcac tattgatgat ggtctggggc ctttttttgc
     2461 cgaacaatcg tgtggacttt gtcatgaagg tgccggacgc gggcctggta aggttttacg
     2521 catagggcga acaaaaaacg gttcatttga cccgatggct gaatttggtg gcccgactat
     2581 acaatttcga accggattaa aagatcgcgt accggagacg gtccccc
//
LOCUS       DAOQNQ010000390         2625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_22241_length_2625_cov_4.217121, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000390 DAOQNQ010000000
VERSION     DAOQNQ010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22241_length_2625_cov_4.217121"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /gene="tyrS"
                     /locus_tag="PK754_15370"
     CDS             <1..870
                     /gene="tyrS"
                     /locus_tag="PK754_15370"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNB10932.1"
                     /translation="KILDAQKTKIVYNSDWLGAMNFADVVKLASRYTVARMLERDDFE
                     NRYRSGEPISIHEFLYPLAQGMDSVALEADVELGGTDQRFNLLVGRDLQREYQQQQQV
                     IITMPLLEGTDGVQKMSKSLNNYIGIDDSPKEMFGRAMSIPDALIYKYFELTTNVDAA
                     ELHKIKTDLDSGTINPSHLKRRLAKELISIYHDDGRAQAAEEEFDLIFKKKEIPEDIP
                     VISLNKGTWIISKLLLECKLVESGKEAQRQVQQGAVSINGEKIADDRAVIQIESEIVI
                     KVGKRKFSKIIPT"
     gene            complement(874..1620)
                     /gene="pssA"
                     /locus_tag="PK754_15375"
     CDS             complement(874..1620)
                     /gene="pssA"
                     /locus_tag="PK754_15375"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNB10933.1"
                     /translation="MKASPKRHLPSLVTALNLFAGFLSVISSIRGDYMEAASLIVVAA
                     IMDVLDGKVARLTKSSSEFGVELDSLADVSSFGFAPAVLIYRAYFEPWGIWGILISFT
                     PLVFGGIRLARFNVGVADHAEKDSYFTGLPIPSSAITIASFVAFEMDVFQACNHIFVM
                     AIVTLLVSFLMVSKIRYEGMPNFSFRKTEDRIKLGLLIVCFIAFLIYPQRVAFPVMIL
                     FIAYGILMGILAIFSNKESDDDTQDIPAID"
     gene            complement(1636..2280)
                     /locus_tag="PK754_15380"
     CDS             complement(1636..2280)
                     /locus_tag="PK754_15380"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HNB10934.1"
                     /translation="MKISREGLVTIFIVNILVSIAIIMYATLGSGWIALVIALLLVEL
                     LVLWFFRDPERTIAGDDNTIIAPADGKVVLIRDIREDKYLKSEATQISIFLSVFNVHV
                     NRYPLGGTIAFYEYMKGKFMVAFDDKASELNEQTVVGLEHPRVKILFKQITGLLARRI
                     VFYGKQGDRVDRGTRCGIMKFGSRMDIIVPKNVDIKVKVGDTTTAGETILGIIK"
     gene            complement(2295..>2625)
                     /locus_tag="PK754_15385"
     CDS             complement(2295..>2625)
                     /locus_tag="PK754_15385"
                     /inference="COORDINATES: protein motif:HMM:NF013428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNB10935.1"
                     /translation="YAFGSANQDEVRTIQRLSGKVNAIVRNFFERRGFQLVDIKLEFG
                     RQKGKIYLADELTLDTCRLWDINRGRKFDKEIAAAKENDLHKIYGEVRQTITHPKTRD
                     ERPTIHD"
BASE COUNT          812 a          542 c          579 g          692 t
ORIGIN      
        1 aagattttgg acgcccagaa aacaaaaatc gtatataatt ctgactggtt aggagccatg
       61 aattttgccg atgtagttaa attggccagt cggtatacgg tagcacgcat gttggagcga
      121 gatgattttg aaaatcgtta tcgtagcgga gaaccgatca gtattcatga gtttttatat
      181 ccattagcgc aaggtatgga ttcggtggcc ttggaagccg atgttgagtt gggtggtacg
      241 gatcagagat ttaatttgct ggtaggccgg gatttgcagc gcgaatacca acagcaacag
      301 caggtcatta ttactatgcc gttattggag gggacagatg gcgttcaaaa aatgagcaaa
      361 tcgttaaata attatatcgg cattgatgac tcgcctaaag aaatgtttgg ccgggcaatg
      421 tctattcctg atgctttaat ttataaatat tttgaattaa ccacgaatgt tgatgcagcc
      481 gagcttcata aaataaaaac agatttggac agtggtacca taaacccatc gcacttaaaa
      541 cgacgcttgg ctaaagaatt gatatctatt tatcacgatg acggtcgtgc gcaagcggcg
      601 gaagaagagt ttgatctgat tttcaaaaag aaagaaatcc cggaagacat tccggttatt
      661 tcattgaaca agggcacgtg gatcatttca aaactactac ttgagtgtaa attggttgaa
      721 tccggcaaag aagcacaaag acaagttcag caaggtgccg tgagtatcaa tggtgaaaaa
      781 atagcggatg atcgtgcagt tatccaaatc gaatcggaga ttgtgattaa ggtaggtaag
      841 agaaagtttt cgaaaattat tccgacttag tcgttagtct atagccggaa tatcttgcgt
      901 atcatcgtca gactctttat tagaaaatat tgccagaata cccatcagaa ttccataggc
      961 tatgaaaagt atcatgacag gaaacgcaac acgttgagga taaatcaaaa aagcaataaa
     1021 acaaactatc aaaagcccaa gtttgatgcg atcttcggtt ttcctgaatg aaaaattagg
     1081 cattccctca tagcgaattt tactgaccat aagaaacgat acaagcaaag tgactatagc
     1141 catcacgaaa atatggttac aggcttgaaa tacgtccatt tcaaatgcta caaaagaggc
     1201 gatggtaatg gccgacgagg gaatcggtaa accggtaaaa tagctgtctt tttccgcatg
     1261 gtctgctacg ccaacattaa aacgtgctaa gcggattccc ccgaaaacaa gcggcgtgaa
     1321 gcttattaag attccccaaa taccccaagg ttcaaaatat gctctataga tcagcacagc
     1381 gggagcaaat ccaaacgatg atacatcggc aagagaatct aattcaaccc caaattcaga
     1441 actcgacttg gtgaggcgag caactttacc gtccaaaaca tccataatgg ccgcaacgac
     1501 aatcagagag gcggcttcca tataatcacc ccgaatggaa ctgatgacag agagaaaacc
     1561 ggcaaaaaga ttgagcgcgg ttacgagcga tggcaaatgc ctttttggcg aggctttcaa
     1621 aagataaatc cctgattact ttatgatgcc gagtatggtt tctccggctg ttgtcgtgtc
     1681 accgacttta actttgatgt ctacgttttt tggaacgatg atgtccatgc gggaaccgaa
     1741 tttcataatt ccacagcgcg taccgcgatc aacacggtca ccttgtttcc cgtaaaacac
     1801 aattcgacgt gcaagaagac cggttatttg cttgaataga atttttacac gtggatgctc
     1861 taggccgacg acggtttgct cattcagctc ggatgctttg tcatcaaaag ccaccataaa
     1921 cttacctttc atatactcat aaaaagcgat cgtcccacct aaaggatagc gattgacgtg
     1981 cacattgaat acagacagaa aaatactgat ttgtgtagcc tccgacttga ggtatttatc
     2041 ttcgcgtata tcgcgtatca aaacaacttt gccgtctgcc ggggcaatga tcgtattgtc
     2101 atcaccggcg attgttcgtt cgggatcgcg aaagaaccat agcacgagca gttcgactaa
     2161 aagcaatgcg atcacgagtg cgatccatcc ggaacccagc gtagcataca tgatgatagc
     2221 tatcgaaacg agtatgttta cgatgaaaat cgttacaagg ccttcgcgcg atattttcaa
     2281 agcaaaatct ccggttaatc gtgaatggtt ggtcgctcat cccgtgtttt aggatgcgtt
     2341 atcgtttgtc gtacttcgcc gtaaatttta tgcaaatcat tttctttagc agcggctatt
     2401 tctttgtcaa atttgcggcc gcggttaata tcccacaaac ggcaagtatc taaggtcaat
     2461 tcgtcagcca aatagatctt acctttttgt cgaccaaatt caagtttgat gtctactaac
     2521 tgaaaaccgc gccgttcgaa gaaattacga acaatcgcat tgaccttacc ggaaagcctt
     2581 tggattgtgc gcacttcatc ctgattggcc gagccgaacg catac
//
LOCUS       DAOQNQ010000391         2624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_22259_length_2624_cov_5.364344, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000391 DAOQNQ010000000
VERSION     DAOQNQ010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22259_length_2624_cov_5.364344"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PK754_15390"
     CDS             complement(<1..222)
                     /locus_tag="PK754_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNB10936.1"
                     /translation="MPEKKTTRRKFLETTGLVATAAAVSPFIRSAEAAISAVSMTDAP
                     FTLPTLNYDYKALEPHIDAQTMEIHHSRHH"
     gene            complement(233..1099)
                     /locus_tag="PK754_15395"
     CDS             complement(233..1099)
                     /locus_tag="PK754_15395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB10937.1"
                     /translation="MIRFALASRIKLLACKTWFMQIASVNKFPYTTILITGASSGIGE
                     AIARALAKPGFRLILVARSADALETHAREFRSSGATVDVIAMDLTSENAPQRLFDQIN
                     KLNIRVDLLVNNAGFGSYGYFHESDIAKQMNMIDLNIKTLVQLTHLFLPGMLSQNKGA
                     ILNVSSTASFQPVPFMATYGATKAFVTHFSVAVASELRDTPIRIISLCPGRTKTNFQL
                     SAGSNKIRIRSRSASKESVAEVALDAVVNYKKIAIEGLGNKYMTHLQRLFPRFVILGL
                     ARRIFEPKNSNT"
     gene            1133..1975
                     /locus_tag="PK754_15400"
     CDS             1133..1975
                     /locus_tag="PK754_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10938.1"
                     /translation="MRFVVFLTTVFVFLNRPSLASFDTKKGARPLAMGGAYTAAAFGG
                     DAFYYNPAGLWYTANPMGQAFFSRPFNLTELQTVTFNFTYPSFFGNVSANAESYGYDL
                     YRETTLSLAYANVYKERVAMGVVINYNHLAIKNAGSAAAVGLDFGILVRPLRMLTVGA
                     MVRNINHPSIKKDALPQTFTVGACFQIMDHFRLMADAYKDVRYPTDIRVGGEYMFMEK
                     FIIRSGLGSDPSRFCGGFGLDLGLAVLDYGFNTHQELGMTHALSLSFHIDRKPSRSLQ
                     PSLR"
     gene            1982..>2624
                     /locus_tag="PK754_15405"
     CDS             1982..>2624
                     /locus_tag="PK754_15405"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNB10939.1"
                     /translation="MVVLLQRISFFIALVFYSSVVVGQDSLWIDTDQDRSTESALESM
                     TETANEGVEFSEALENWRRNPLDINRADIEELQQLPGLSPILAKAIIDRRRAQPFTSI
                     RQLLQVDGFSRELFVRIQPFLIVYSHTAVRPFDVALRQRTERQIEKTPEFSDGSYEGG
                     PLKMYERAVAVYAPGWSALPDAQIRGAWVMEKDPGETRWNDHQAGYAEIKNAGV"
BASE COUNT          619 a          594 c          679 g          732 t
ORIGIN      
        1 atgatggcgg gaatgatgga tctccatggt ttgggcatca atatgcggtt cgagcgcttt
       61 gtaatcatag ttcagcgtcg gcaatgtaaa cggtgcatcc gtcatgctta cggcagaaat
      121 cgcggcttcg gcggaacgga taaaggggct taccgccgcc gccgttgcaa ctaaaccggt
      181 tgtttccaaa aatttgcggc gtgttgtttt tttttcaggc ataaacggct ccttatgtgt
      241 tggagttttt tggttcaaaa atgcgccgtg caaggccaag aatgacaaag cgcggaaaga
      301 ggcgctgtag gtgtgtcata tatttattgc ccagcccttc gatggcgatt tttttataat
      361 tcactacggc atccaaagcg acttcggcta ccgactcttt ggacgcactg cgtgagcgga
      421 tgcgaatttt gtttgatcct gccgagagtt gaaaattagt tttcgttcgt ccggggcaca
      481 ggctgataat tcgtatcggc gtgtcacgta attccgacgc gacggccacg ctgaagtggg
      541 tgacaaatgc tttcgttgca ccatacgtcg ccataaaagg gacgggttgg aaacttgccg
      601 tggaacttac gttgaggatg gcgcctttat tttgggagag catgccgggt aaaaaaagat
      661 gcgtgagctg gacgagcgtt ttgatattca ggtcaatcat gttcatctgc ttagcaatat
      721 cggattcgtg aaaatatccg tatgaaccga atccggcatt atttaccagc aaatcaacgc
      781 ggatgttaag tttgtttatt tggtcaaata aacgttgcgg tgcgttttcc gatgtcaaat
      841 ccatcgcgat cacatctacc gttgcaccgg atgagcgaaa ttcgcgcgca tgggtttcga
      901 gtgcatcggc cgaacgcgca accaagatca aacgaaagcc gggtttggcc agcgctcggg
      961 ctatcgcttc accgattccg ctggaagcgc cggtgatcaa aatcgtggtg taaggaaatt
     1021 tattcacgct tgcaatctgc ataaaccatg ttttgcaagc aagcaatttt atacggcttg
     1081 ctaatgcaaa acgaatcatt taatttccac aagcctccta aaataaatac ctatgcgttt
     1141 tgtagttttt cttacaaccg tttttgtgtt tctgaacaga ccgtcgttgg catcttttga
     1201 taccaaaaaa ggtgctcggc ccttggccat gggcggggca tatacagccg cggcgttcgg
     1261 aggggatgcg ttttattata atcccgccgg gctctggtat accgccaatc cgatgggaca
     1321 agcctttttt tcacgcccct tcaatcttac cgaacttcaa accgttacgt ttaacttcac
     1381 gtatccgtcc ttttttggga atgtgagcgc caatgccgaa tcgtatggtt acgatttata
     1441 tcgtgaaacg acgttgagtc tggcttatgc taacgtatat aaggaacgtg tggcgatggg
     1501 tgttgtgata aactataacc atcttgccat caaaaacgca ggctcggcgg cggccgtcgg
     1561 attggatttt ggtattttag ttcgaccgct gcgaatgcta accgtcggtg cgatggttcg
     1621 caatatcaac catccttcga taaaaaaaga cgcattgccg cagacattta cagtcggcgc
     1681 ttgttttcaa atcatggatc attttcgcct catggccgat gcgtataaag atgtccgtta
     1741 tcccaccgat attcgtgtcg gcggggaata catgtttatg gaaaaattta tcatacgcag
     1801 cggcttaggt tccgatccgt cccgtttctg cggcggattt ggcttggatt tagggttggc
     1861 cgttttggat tatggtttta atacgcatca ggagcttgga atgacacacg ctctgtctct
     1921 cagttttcat attgaccgaa aaccctcgcg ctcgcttcaa ccgtcattgc ggtaatcatc
     1981 tatggttgtt ctgctccaac gtatttcttt ttttatcgcg ttagtttttt attcgtccgt
     2041 cgtcgtcggc caggattcgc tgtggattga taccgatcag gatcgcagca ccgaatccgc
     2101 tttggagtca atgacggaga cggcgaatga gggtgtggaa ttttcagagg cgctggaaaa
     2161 ttggcggcgt aatcctttgg atattaaccg cgcggatatt gaagaattgc aacaattacc
     2221 cggattatca ccgatcttgg ccaaagcgat cattgatcgt cggcgcgcgc aaccttttac
     2281 atctatccga caacttttgc aggtcgatgg gttttcaagg gagttgtttg tacgtattca
     2341 accgtttctc atcgtgtaca gtcataccgc ggtcaggccg tttgatgtcg cattgcgcca
     2401 acgtaccgag cgccaaattg aaaagacgcc ggagtttagc gacgggagct atgaaggcgg
     2461 cccgcttaaa atgtacgaac gtgccgttgc cgtatatgca ccgggatggt cggcgttgcc
     2521 tgacgcccaa atacgcggcg cttgggttat ggaaaaagat cccggcgaaa cccgatggaa
     2581 tgaccatcag gccggttatg ccgagataaa aaatgccggt gtag
//
LOCUS       DAOQNQ010000392         2622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_22288_length_2622_cov_5.240748, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000392 DAOQNQ010000000
VERSION     DAOQNQ010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22288_length_2622_cov_5.240748"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            495..1379
                     /locus_tag="PK754_15410"
     CDS             495..1379
                     /locus_tag="PK754_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNB10940.1"
                     /translation="MINHSRRRFIAAAALLGAAASGSGKPVQAASRNELSPDRRGVLV
                     DTTVCIGCRTCEWACRKAHGYDDEKLENYQSTDVFKKVRRPDETRLTVINQYSQQQNE
                     TFVKFQCMHCDEPACVSACIVGAFHKEEKGSVLWDTDKCIGCRYCMVACPFQVPAFEY
                     EKAFRPDIIKCDFCKQRTDKGERPACVAECPVEAMIFGTRAELLQIAKDRIRRHPEKY
                     KDHIFGETEVGGTAWLYLAERDFAEIGFPKLGRNPAPGVSESIQHGIFAYFVPPVSLY
                     ALLGGIMWLMKHREEPDD"
     gene            1389..>2622
                     /gene="hybB"
                     /locus_tag="PK754_15415"
     CDS             1389..>2622
                     /gene="hybB"
                     /locus_tag="PK754_15415"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HNB10941.1"
                     /translation="MNAFEKPVPVEKKFFTAGVMILILLALNGIVFLAGRFLFGLGAV
                     TNLDNQYPWGLWIGVDVAAGVALAAGGFTSAALGHIMHREEYHAVIRPALLTAMLGYT
                     FVAMGVCIDIGRYYYIWHPLIMWNGTSALFEVGMCVMIYMSVLYIEFLPIVTERFIGR
                     VALTGILKALNKPLDRFLRLLDRGLSKSMFVFIIMGVVLSTLHQSSLGTLMVIAGAKM
                     HPLWQTPILPLLFLLSAVSVGFPMVIFESLLAARSFGLKPEMAVLSRLARIVGPLLAI
                     YLAFKIGDMFIRETFVYLNEPTLESMMFVIELVVGVIIPLRMFLSPGVRRSPLWLFVA
                     SGLVILGVLINRINNFIVAYTPPYATERYVPSFGEISVTVGFVALLVLVYRVCVMVFP
                     VISKPDPNGGPARKYAIKG"
BASE COUNT          646 a          537 c          642 g          797 t
ORIGIN      
        1 cgtttaataa agaccataca acccaaagaa accgtcgatg atctttggag tgttgccggc
       61 aaatcgtcaa gacttcaatt accaaacctt attttcaaag ttaataatta gcaacaacta
      121 ataacaaccc atgtgtgtct atggccggtg aatataaaat tttgccggct ttattatttc
      181 acataatcaa atttataaca acagactcca taatctgaat tattttttcc ttccttttat
      241 catgaaatct caaaattggc gccagaaata aaattttatg agtgtcttgt aatacttttg
      301 ctctatttga cctcacgggt cacgtgctta catgatttaa agcggctatt tttcaagcga
      361 aaaagtttga tcatcactga ccggtatcat tgtagtaaaa cgccttcaaa cgtatgttgt
      421 atgtgcattg ttgaaattgt tttttgaaac gattggaatt gcaatagtta cttcttcatg
      481 ctgcgaggca aattatgata aaccacagtc gccgaaggtt tatcgctgcg gcagcgctgc
      541 tcggtgcagc cgcttcagga agcggtaaac ccgtacaagc ggcttcgcgc aatgagcttt
      601 ctccggatcg ccggggtgtt ttggttgata ctaccgtatg tatcggttgc cggacttgcg
      661 aatgggcttg ccgcaaagcg cacgggtacg acgatgaaaa actggagaac tatcaaagca
      721 cggacgtatt taagaaagtt cgccgccctg atgaaacccg cctgactgtc attaatcagt
      781 attctcaaca acaaaatgaa acttttgtga aatttcagtg catgcactgt gatgaaccgg
      841 catgcgtttc agcgtgtatt gtcggcgcgt ttcacaaaga agaaaagggt agcgttttgt
      901 gggatacgga caaatgtatc ggttgccgat actgtatggt agcgtgcccc tttcaggtgc
      961 cggcgtttga atatgaaaaa gcgtttcgtc ccgatattat caaatgtgat ttttgcaaac
     1021 aacgtaccga taaaggagag cgtcccgcgt gcgttgcgga atgtccggta gaagctatga
     1081 tattcggtac acgcgctgaa ttattacaaa tcgcaaaaga tcgcatacgt cgtcatcctg
     1141 aaaaatataa agatcatatt tttggagaaa cggaagtcgg gggcacggcg tggttgtatt
     1201 tagccgaacg cgattttgct gaaatcggat ttcccaaact cggtcgcaat ccggcgccgg
     1261 gcgtttcgga atccattcag catggcatct ttgcctattt tgtgccgccg gtatcgcttt
     1321 acgccctttt gggcggcatt atgtggttga tgaagcatcg ggaagaaccg gatgattagg
     1381 agaagcatat gaacgcattc gaaaaacctg tgcccgtgga aaagaagttt tttacagccg
     1441 gtgttatgat actgatattg ctcgccctga atggaatcgt attcctggca gggcgttttc
     1501 tttttggctt gggtgcagtg accaatctgg acaatcaata tccatggggt ttgtggatcg
     1561 gggtagatgt ggcggccgga gtcgcattag ccgccggagg gtttaccagc gccgctttag
     1621 ggcacatcat gcatcgggag gaataccacg cagttattcg tccggccttg ctcacggcga
     1681 tgttaggata cacgtttgtc gcaatgggag tctgcataga tataggacga tattattata
     1741 tctggcatcc tttgatcatg tggaatggca cctcagctct ttttgaagtg ggtatgtgcg
     1801 tgatgatcta tatgagcgtg ctgtatatcg agtttttacc cattgtgacg gaacgtttta
     1861 tcgggcgtgt ggcgctaacc ggaattttga aagcacttaa taaaccactg gatcgttttt
     1921 tgagattact ggatcgcgga ctgagtaaat ccatgtttgt atttattatc atgggcgtcg
     1981 tgttatctac gctgcaccag tcctcattag gaactctgat ggttatcgcc ggagctaaga
     2041 tgcatccgct gtggcaaacg ccgatactgc ctttattatt tttattgtcg gccgtatctg
     2101 tgggatttcc gatggttatt tttgaatcat tgttagcggc acgttctttt ggtttgaaac
     2161 cggaaatggc agtgctttca agacttgctc gtatcgtcgg cccattgtta gcgatctatc
     2221 tggcgtttaa aatcggggat atgtttatcc gcgaaacatt tgtgtatctc aatgagccga
     2281 cccttgaaag tatgatgttt gttattgagt tggtcgtagg cgtaatcatt ccgcttcgca
     2341 tgtttctttc accgggtgtg cgccgttcac cgctatggct tttcgtcgca tccggcctgg
     2401 taatattggg tgtactgatc aatcgtatca ataattttat cgtcgcttat acaccgcctt
     2461 atgcgaccga gcgatatgtg ccatcgtttg gagaaatatc ggttaccgtc ggatttgtag
     2521 cgttacttgt attggtttat cgtgtttgtg ttatggtatt tcctgttatc agcaaacccg
     2581 atcccaatgg tggtccggcc cgtaaatatg cgattaaagg gt
//
LOCUS       DAOQNQ010000393         2616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_22395_length_2616_cov_5.349863, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000393 DAOQNQ010000000
VERSION     DAOQNQ010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22395_length_2616_cov_5.349863"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="PK754_15420"
     CDS             <1..710
                     /locus_tag="PK754_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10942.1"
                     /translation="FLIVVDMDGTRIQKLGFDGHFQMGVVSSEYGFNKVNLTSVAIDG
                     FNNVWVTDAENHAIHKFDRDLRYMGTFGSPGDGENQFIEPRAIAIGKKFGQVFIADKI
                     SAQYYHIGTDIQNLNVAIKDSQLVFSFYLPETSRLTARIVDEKGKLVATLARNSMTSP
                     GIKMLQWNRAYKSNSPLRDILQFREQNRSDSTQGKQPAMTEQLAPSGIYKIEIEAKST
                     YIYNRYFTAKRALEFAF"
     gene            725..1018
                     /locus_tag="PK754_15425"
     CDS             725..1018
                     /locus_tag="PK754_15425"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HNB10943.1"
                     /translation="MIDHIVLFKLKPGADTTAIENMCTALEDLKHRIPQVIEIHARPN
                     FSERRQGYDIMLTSRFKNKEDLRIYADHPEHLRVVKELVAPIRESVIVGDIEF"
     gene            complement(1015..>2616)
                     /locus_tag="PK754_15430"
     CDS             complement(1015..>2616)
                     /locus_tag="PK754_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10944.1"
                     /translation="VQQWTVENPDAGTVLDLTNQGTYDDWVRWFLYETQGWPVSEHDG
                     ETVLHNEWARGQYDFLMRLIFCDDTTMTDLMTIPISSLVGLRYVQNMEVRLNELIQKA
                     SEKVFILKSEENLVQNELSLLEQKRQMVLVLEKDLESDTELLQKLDERLIQTQHERVQ
                     AERITGYIQEKRSEITKLGFEITTYQNVMQNNVLQDTSEEEIRTMDRKARLYEKAIKE
                     IETIEENIIQRRHLEKNLEGIEQEIQVIDRTHTGKLPPEIKTQKKAYESEARRLRLLL
                     SKMQDDPSSMEHWQTEKRAHEEAYHQYTILKKTRQYMNQNNLQKLKSTTDKLIVKRQH
                     MQDELDQIFASQHETQYQKLEAAVASDRQQALELKERMATAVGRKEKLQQDIQALQLV
                     VDKHLSRADQKRREITSKRHLLFVRETVTDFYNAQIELRKNELSRQIKTHLKKFRVAF
                     TSKQLFGKNKTNEPSLETIPISTSERILSEHIFAMTVLAMNLKNPIFFADENFSWYLT
                     KDDRTEIEALADQLNFGQFILFEKN"
BASE COUNT          686 a          573 c          526 g          831 t
ORIGIN      
        1 actttcttat cgtcgttgac atggatggca cacgcattca aaaattagga ttcgatgggc
       61 attttcaaat gggtgtggtt tcatcagaat atggcttcaa caaagtcaat ctgacatcgg
      121 tagctattga cggttttaat aatgtttggg ttaccgacgc agaaaatcat gccattcaca
      181 agtttgatcg cgatcttcgt tatatgggca ctttcggttc gcccggtgac ggtgaaaatc
      241 aatttataga acctcgtgcc atagctattg gaaaaaaatt tggtcaggta tttattgccg
      301 ataagatcag cgcacagtat tatcatatcg gaacagacat acagaatctc aatgtcgcga
      361 tcaaggatag ccagttagta ttttcatttt atttaccgga gacatcccgt ctgacagctc
      421 gcatcgtgga tgaaaaagga aaacttgtcg ctacgcttgc ccgtaatagc atgacatcgc
      481 cgggaatcaa aatgctacag tggaacagag cttataaatc caacagcccg ttgcgggaca
      541 ttttacaatt tcgcgaacaa aacagatccg attctacaca aggcaaacaa cccgctatga
      601 ccgaacaatt agcgccttca ggaatatata aaatagagat tgaggccaaa tcaacctaca
      661 tctataaccg ttattttacc gccaaacgcg cattagaatt tgctttttaa acaaggagga
      721 taccttgatt gatcacatcg ttttattcaa actaaagccg ggtgcagata ctactgctat
      781 cgaaaacatg tgcactgctt tggaagatct gaaacatcga ataccgcaag tcattgaaat
      841 tcacgcaagg cctaattttt ccgagcgccg ccaagggtac gatattatgc ttacaagtcg
      901 ttttaaaaat aaggaagatt tgcgaattta tgccgatcat cctgaacatc tgagggtcgt
      961 taaggaactc gtcgcgccga tacgcgaatc ggtcattgtg ggcgatatag aattttaatt
     1021 tttttcgaac aaaatgaatt gaccaaaatt taattgatcg gccaatgctt cgatctccgt
     1081 acgatcatct ttcgtcagat accacgaaaa gttctcatca gcaaaaaaaa taggattttt
     1141 taaattcata gccagaactg tcatggcaaa aatatgctca gaaagaatcc gctctgatgt
     1201 ggatatcggt atcgtttcaa gcgaaggttc gtttgttttg ttttttccga acagttgttt
     1261 actcgtaaac gcaactctaa atttctttaa atgcgtttta atttggcggc tcagttcgtt
     1321 ttttcgaagc tcgatttgcg cgttataaaa atccgttacc gtctcgcgaa caaaaagcaa
     1381 atgccttttt gatgtgattt ctcgacgttt ctgatcggcc cgcgaaaggt gcttatccac
     1441 gacaagttga agcgcttgta tatcctgctg taatttctct tttcttccaa cggccgtagc
     1501 catccgctct ttcagttcga gcgcttgctg gcgatccgat gcaacggccg cttccagttt
     1561 ctgatattga gtttcgtgct gggatgcaaa tatctgatcc agttcatcct gcatgtgttg
     1621 gcgcttcacg atcaatttat cagtcgttga tttcagcttt tgcaggttat tttggttcat
     1681 gtactgacgc gtttttttga gaatcgtata ctgatgatac gcttcttcat gcgcgcgttt
     1741 ttcagtttgc caatgctcca tcgaagaagg atcatcctgc attttggaca gcaacaagcg
     1801 taaacgacgc gcttcggact cgtacgcttt tttttgcgtt tttatttccg gaggtaattt
     1861 gcccgtatgc gtgcgatcta tgacctggat ctcctgctcg ataccctcga gatttttttc
     1921 taagtgccgt cgttgtatga tgttttcttc gatggtttct atttctttga tggctttttc
     1981 atataaccgt gctttacgat ccatcgtacg tatttcttct tccgacgtat cttgcagtac
     2041 gttgttttgc atgacgtttt gatacgtcgt aatttcaaaa cctagtttgg taatttcgct
     2101 gcgcttttcc tgaatatagc cggtgatgcg ctcggcttgt acgcgttcgt gttgcgtttg
     2161 aatcagtctt tcgtccaatt tttgaagtaa ttcggtatcg ctttccaaat ccttttccaa
     2221 cactaaaacc atttgtcgct tttgttctaa taatgacagt tcgttttgga cgagattttc
     2281 ttccgatttg agaataaata ctttttccga cgctttttgt atcaattcgt ttaatcgtac
     2341 ttccatgttc tgtacatacc gaaggccaac caaagaagaa atcggtatcg tcatgagatc
     2401 agtcatagtc gtatcatcac aaaaaataag gcgcatcaaa aaatcgtact gcccacgcgc
     2461 ccattcattg tgcaaaacgg tctcaccatc atgttcgctc accggccaac cttgcgtttc
     2521 atacaagaac catcgcaccc agtcatcata cgttccctgg tttgtaaggt ctaaaaccgt
     2581 gccggcatcc ggattttcta cagtccattg ttgtac
//
LOCUS       DAOQNQ010000394         2610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_22495_length_2610_cov_4.252838, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000394 DAOQNQ010000000
VERSION     DAOQNQ010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22495_length_2610_cov_4.252838"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PK754_15435"
     CDS             <1..388
                     /locus_tag="PK754_15435"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNB10945.1"
                     /translation="KTAKQRFNQINEFEELQAENGVTIIKFFLHISKEEQRKRLQERL
                     DDKSKHWKFSANDLKERAMWGQYMDAFEDVFNHTSKKHAPWYVIPSNKKWFRNLATAN
                     ILVDTLKSLDMKYPKPKQDFSNLVIE"
     gene            525..>2610
                     /gene="tkt"
                     /locus_tag="PK754_15440"
     CDS             525..>2610
                     /gene="tkt"
                     /locus_tag="PK754_15440"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNB10946.1"
                     /translation="MAPNTLESLCINTIRTLSMDAVQQANSGHPGTPMALAPIAFTLY
                     DKFMKHNPGNPHWPNRDRFILSAGHASMLLYSTLHINGYGISLDDIKQFRQLHSKCAG
                     HPEHGLAPGIETTTGPLGTGVANSVGFAIAEKWLAQYFNRPGYEILNYSIFAVAGDGC
                     MMEGISGEAASLAGHLGLDNLIWIYDNNKITIEGSTSLAFSEDVMKRFEAYGWFVQHI
                     TDANDTNALSSAIERAQQTKGKPSFIMVDSHIGYGAPHKQDSHSAHGEPLGEEEIKAT
                     KQVYGWDPEKKFFVPDDVKAYSQFAVEKGSQHEQEWQKKFEAYRTAFPDLAKQFELIQ
                     KRELPEGWDADLPVFPADAKGVAGRDAGGQLINALAAKIPWLVGGAADLAPSTKTLIK
                     GAKSFSRDNRDGRNFHFGIREHAMGAIVNGMTLSGLHAYGSGFLIFSDYMRPTFRLAA
                     LMHQPSLFVFTHDSIGVGEDGPTHQPIEQVGSLRAIPNLEVIRPADANEVAEAWKYMI
                     RLKDKPAALILTRQALPTMDRTKYAPASGAQRGGYILADCKGKPDIILIGTGSEVQLC
                     IGAYEKLTAEGYKCRVVSLPCWSLFERQGKAYWEKVLPSDVRCRVAVEAAASFGWRRY
                     TGIEADGGIIAMRSFGESAPIKALLEEFGFTTEAVVKLAKQKLKQFGKAAPKKTVSAK
                     NKPAKKAKSKPKS"
BASE COUNT          702 a          601 c          685 g          622 t
ORIGIN      
        1 taaaacagca aaacagcggt ttaatcaaat caacgaattt gaagagttgc aggcggaaaa
       61 cggtgtcacg atcatcaaat tctttctgca tatttccaaa gaagagcagc gcaagcgtct
      121 ccaggaacgg ttggacgata aatccaaaca ttggaagttt tcagccaacg atctcaaaga
      181 acgtgcgatg tggggacaat acatggatgc attcgaagat gtatttaacc acaccagtaa
      241 aaaacatgcg ccgtggtatg tgattccttc caataaaaaa tggtttcgta atttggctac
      301 ggccaatata ctggtagata cgctcaaatc ccttgatatg aagtatccca aacccaagca
      361 ggatttttca aatcttgtga tcgaataaaa ttagtttgat tttttttcac aaatccccgg
      421 gcataaaccg gggatttgtg ttataaaata ttccgttgat ctatcgcaac ggatagtgta
      481 actttggggc gtcctttcaa tcataccttt gggagaaaaa cttcatggct ccgaataccc
      541 ttgaatcgct gtgtattaat accattcgta cactgtctat ggatgcggta cagcaggcca
      601 attccggcca tcccggaaca ccgatggctc tggcgccgat cgcgtttacg ctgtatgata
      661 aatttatgaa acacaatccg ggcaatccgc attggcccaa ccgggatcgt tttattttat
      721 cggcagggca tgcatccatg ctgctttaca gtacactgca catcaacggg tatggtattt
      781 cgctggacga tatcaaacag tttagacaac tgcattccaa gtgtgcgggg catcccgagc
      841 acggtctggc tccgggcatc gaaacgacaa cggggccttt gggaacgggc gtggcgaatt
      901 cggtcggctt tgccatcgct gaaaaatggt tggcgcaata tttcaatcgc cccggctatg
      961 aaattttgaa ttacagcatt tttgccgtgg ccggtgacgg atgtatgatg gaaggcattt
     1021 ccggcgaagc cgcatcgctg gcgggccacc tcggattgga taatctcatt tggatctatg
     1081 ataacaataa aattacgatt gaaggttcga catcgctggc gttcagcgaa gatgtgatga
     1141 aacgttttga agcgtacggt tggtttgtgc aacatatcac cgatgccaat gataccaacg
     1201 cgttatcgtc cgccatcgag cgcgctcagc aaaccaaagg taaaccgtcg tttatcatgg
     1261 tggatagtca catcggatac ggtgcaccgc acaaacagga ttcgcactcg gcgcacggtg
     1321 aaccgctcgg cgaagaggag attaaagcga cgaaacaggt ttacggttgg gatcccgaaa
     1381 aaaaattctt tgtgccggat gacgtaaaag catacagcca atttgccgta gaaaaaggtt
     1441 cgcaacacga gcaggaatgg cagaaaaaat ttgaagcgta ccgcactgcg tttccggatc
     1501 tggccaaaca atttgagtta attcagaaac gggaattacc cgaaggttgg gatgccgatc
     1561 tgccggtatt tccggccgac gccaaaggcg ttgcggggcg tgatgccgga ggccaattga
     1621 tcaatgcctt agcggccaaa attccctggc ttgtcggtgg cgccgcggat cttgcaccct
     1681 ctacgaaaac tctgatcaaa ggcgcaaaaa gttttagtcg tgacaatcgc gacgggcgaa
     1741 attttcactt tggtattcgt gaacatgcca tgggcgccat cgtcaacggg atgacactca
     1801 gcgggttgca tgcgtacggt tcgggttttc tgattttttc ggattatatg cgacccacat
     1861 ttcggttggc ggcgctgatg caccagccgt cgttgtttgt tttcacgcat gacagtatcg
     1921 gcgtcggtga agacggcccg acccaccaac cgatcgaaca agtcggttcg ttgcgcgcta
     1981 ttcctaatct tgaggtgatc cgtccggcgg atgccaatga agtagcggaa gcatggaaat
     2041 atatgatacg acttaaggat aaaccggcgg ctctgatttt gacacgccag gccttaccga
     2101 ccatggatcg cactaaatac gcaccggctt ccggtgcgca acgcggcgga tacattttag
     2161 ccgactgcaa aggtaaaccg gatatcattc tgatcggtac gggttccgag gtacaactgt
     2221 gtatcggggc ctatgaaaag ctgacggcgg agggttataa atgccgcgtg gtgagcctgc
     2281 cgtgctggag tttatttgaa cgtcaaggca aggcgtactg ggaaaaagta ctgccgagcg
     2341 atgtgcgttg ccgtgtagcc gttgaggccg cagccagctt cggatggcgg cgttatacgg
     2401 gtatcgaggc cgatggcggt atcatcgcca tgcgcagttt tggcgaatcc gctccgatca
     2461 aagcgttgtt agaagagttc ggttttacta cggaagcggt ggtgaaacta gccaaacaga
     2521 aactgaaaca atttgggaaa gccgcgccta aaaaaacagt aagcgctaaa aacaaacctg
     2581 ctaaaaaagc caagagcaag ccgaagtcta
//
LOCUS       DAOQNQ010000395         2608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_22527_length_2608_cov_4.474736, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000395 DAOQNQ010000000
VERSION     DAOQNQ010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22527_length_2608_cov_4.474736"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PK754_15445"
     CDS             <1..332
                     /locus_tag="PK754_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10947.1"
                     /translation="SYCVRKMLDNPGVDYASFIEKWSSKSKIENKDFVEKKELIPSLS
                     SSPWIFSENYPPLQFQKKYPSGTLLQSWDRVMDVIRHYYPKAKIAVVTCAPVQIPVLK
                     GNDESVD"
     gene            316..1506
                     /locus_tag="PK754_15450"
     CDS             316..1506
                     /locus_tag="PK754_15450"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNB10948.1"
                     /translation="MKALIDARNAAGMLKLFDITLVERLLRQVWVRGIREIIILGSIN
                     PSVFRTEFSKRFPMTVTIIEQYEEWLSKTGDLVILQSDVLYDDRILDVLTQTQDDRLI
                     GDFNNPSCPYGAHLSGINITVEQVLLENNVRRLFSNENIQDIHDMDVYIKFLRKSYVP
                     TLKRIHVGDDLTDIENDLFEKTFKSGLEWIAIYGYKTPVRELTRLFSKTNITPNHVTA
                     VAVFCRWVSIPFLFYGWIGLGLGLIVLFVLLDSLDGKLARMTFRFSDQADWIDHGSVL
                     PTRLGWYAGLGWHYSSGVWSSQAGMLTIITLVCIVLDDLNWAVAKRIFKRTLFDITDF
                     DAKAHLFTHRRNDMFLMLSGYLIGLGLYSFYLICGWVVVTWIWHCTRIVYLSNDSSLS
                     KERN"
     gene            1524..1886
                     /locus_tag="PK754_15455"
     CDS             1524..1886
                     /locus_tag="PK754_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10949.1"
                     /translation="MLKLTAHILLTNDFEQYAHREIYGVTFVERLIRQCKELKAHTIN
                     VWSEKIMFVHSDVYCHPFNEWNQYMSQLRVREEERIIIIDSNIIADDRLKISGQSHGF
                     VGNYKSVPHSWSKYFQIG"
     gene            1969..>2608
                     /locus_tag="PK754_15460"
     CDS             1969..>2608
                     /locus_tag="PK754_15460"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNB10950.1"
                     /translation="MNKYVHELRLQFVPYFFVYTHALDIRQMQNIMYEANFKGTLDFI
                     ATYVYKYPVREITKLLSRHPWVTPDFITVLSIASSFAVPLLLAFGEMGWAVLVGWLMF
                     IFDSVDGKLARLTVRLNPTMGVIEHATSAPAIFLWFAGLGWYFSNGQLLEFTHPASAT
                     AWSLMALYWLDKGVNGIFKPRFGIDLYNYAPIDRFFHLFACRRAIIHLIITLG"
BASE COUNT          727 a          477 c          578 g          826 t
ORIGIN      
        1 gatcgtattg cgttagaaaa atgcttgata atcccggcgt agattatgct tcgttcatcg
       61 aaaaatggtc ttccaaatca aaaatagaaa acaaggattt tgtggaaaaa aaagagctaa
      121 ttccttcgct cagtagtagt ccgtggatat tttcagaaaa ctacccgccg ctacaattcc
      181 aaaaaaaata tccgagcgga acacttttac aatcatggga tagagtcatg gatgtaatcc
      241 gacactatta tcctaaagcg aagatagccg tagtgacttg tgctccagtt caaatacccg
      301 tattgaaagg aaacgatgaa agcgttgatt gatgcccgaa acgcagccgg catgcttaaa
      361 ctatttgata ttactcttgt cgaacgattg cttcgtcaag tgtgggtacg tggaatacgc
      421 gagatcatta ttttaggctc aatcaatcct tccgttttta gaacggaatt ttctaaacga
      481 tttccgatga cggtgaccat tatcgaacaa tatgaagaat ggttatcgaa aacaggggac
      541 ctcgtaatac tgcaaagcga tgtgctgtac gatgaccgaa tacttgatgt gttaactcaa
      601 acacaagacg atagattgat aggtgatttt aataatccgt cttgccctta tggtgctcat
      661 ctttcaggaa taaatatcac tgttgaacaa gtattattag aaaataacgt tcgtcggtta
      721 ttttctaatg aaaacatcca agatattcac gacatggatg tctatatcaa attcttaaga
      781 aaatcctatg tgccgacgct aaaacgtatt catgtcggtg atgatctgac agatattgaa
      841 aacgacctgt ttgagaagac atttaaatcg ggtttagaat ggatcgctat atacggttac
      901 aaaacgccgg tacgcgaact cacacgcctt ttttccaaga caaacattac gccaaatcat
      961 gtaacggctg ttgcggtttt ctgccgatgg gtatcaattc cttttttatt ttatggttgg
     1021 attgggttag gactgggtct gatagtgttg tttgtcctat tggattcttt agatggcaaa
     1081 ctggcacgta tgacgtttag atttagcgat caagccgatt ggattgatca tggttccgtt
     1141 ttaccgacac gtcttggctg gtatgcgggg ttgggatggc actacagttc tggtgtatgg
     1201 tcgtcacaag cgggtatgct aactatcata acattagttt gtatagtttt ggatgacctc
     1261 aattgggctg ttgccaaacg aatttttaaa cgtactcttt ttgatattac tgattttgat
     1321 gcaaaagcgc atttgtttac tcatcgccgc aatgatatgt ttttgatgct tagcggttat
     1381 cttatagggt tagggctgta ttcgttttat ttaatttgcg ggtgggtcgt ggtcacgtgg
     1441 atctggcatt gcacgcgaat tgtctattta tcgaacgata gtagcttgtc aaaagaaaga
     1501 aattaataaa tcgagatcta aaattgttga aattaacagc ccatatatta cttacgaatg
     1561 attttgagca atatgctcac cgagagattt atggagtaac attcgttgaa aggcttattc
     1621 ggcagtgtaa agaattaaaa gcgcatacca ttaatgtatg gagtgaaaaa attatgtttg
     1681 ttcactcgga tgtatactgt cacccgttca atgaatggaa tcaatatatg tctcaactgc
     1741 gagttcgtga agaagagcga attattatta ttgattctaa tatcatcgcg gatgatcgtt
     1801 tgaaaatatc tgggcaatca cacggattcg ttggcaatta caaatcagta ccacatagtt
     1861 ggagtaaata cttccagatt ggttaaaaat aacgtcgatg ttttttcctt tgaaaccgcc
     1921 aagaagtatg tggctaattt tgatccaaaa gtgatagagc cgaatgaaat gaataagtac
     1981 gttcatgagt tacgcttgca attcgtgcct tactttttcg tatatacgca tgctttggac
     2041 attcgtcaaa tgcaaaacat catgtatgaa gctaacttta agggtacact cgattttatc
     2101 gccacctatg tgtataaata tccggtccgc gaaatcacta agttgttaag ccgtcatccc
     2161 tgggtgactc cggattttat caccgtgctg agtatcgcta gttcgtttgc tgtcccgtta
     2221 ctattagcct ttggagaaat gggatgggcc gttttggtgg gttggcttat gttcattttt
     2281 gattccgtgg acggaaaatt ggcgcgactg accgttcggc ttaacccgac gatgggcgtg
     2341 atcgaacacg ctacgagtgc cccggctatt tttttgtggt ttgccggtct gggctggtat
     2401 ttttcaaatg ggcaactttt agagtttacc catccagcct cggctacggc gtggtcgctg
     2461 atggcgctgt attggttaga taaaggggtc aatggaatat tcaaaccgcg cttcggtatt
     2521 gatttgtata actatgctcc gatagatcgt ttctttcacc ttttcgcctg tcgtcgcgcg
     2581 attatacatc ttatcattac cctagggt
//
LOCUS       DAOQNQ010000396         2581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_22958_length_2581_cov_3.460412, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000396 DAOQNQ010000000
VERSION     DAOQNQ010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22958_length_2581_cov_3.460412"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1463
                     /locus_tag="PK754_15465"
     CDS             171..1463
                     /locus_tag="PK754_15465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB10951.1"
                     /translation="MTKSAYTGLLILILFVMAVSWDGRREKMAPLSKSAAISIKRVST
                     GLSTVGADSANIGAWVEHRLEGSTNANALYQGFIAFHFDEMDTMLTVHSPAGRTNAGI
                     FSGDKPTFSKTGYTASLQTDLSEEHYTVSTQPIANFSKIKLTQILTAPKNNERIVYLD
                     FIFEFAPKDSLTDPRQYSNFRFLMGYDGDIGTTTGGMNNDSTGYYGGNQTGLAYIYDA
                     VLSLYSGVALHEKGVSDSAGNFGLWHQTVNANEFSTVKLDTLMHEMMMHPSWNSTVQN
                     TDAMVYWTLNRGTIVPDDFVPYTDTIRFTLVNGLSKTTVIEASKGIHVPDVLPPDEPS
                     SPLPTTFVLFNNSPNPFNPSTTIRFYIPEPMFVRLTVYNLLGQKVRTLIARAVEPGIS
                     QVVWDARNDVGQTVAAGVYLYRMETPQGIITRRMLLLK"
     gene            1474..>2581
                     /locus_tag="PK754_15470"
     CDS             1474..>2581
                     /locus_tag="PK754_15470"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HNB10952.1"
                     /translation="MKIQTKLSVLLAAVLIFLVSAVAVFQYVDGQRIEQLYRREITER
                     SIVFDQIVSLNNKSLELFVSDYSNWDDMVTFIHKPDSVWAESNIDAVLSKFNVQLFLL
                     CDRSFNVVYTTHINYTENNELIRTVTRDFQKSANSGLFKHFYVLIGDTILEIRTAPVQ
                     PSMDVHRTTEPAGYLFVGRVWDDRYVNELADATQSDMYISTSEASLLNDRVLPRDTIV
                     VTRTFKNLSEKTIGWVSSIQKAEEFTELDRQANLQFILLVVFSTVLFLPLSLLLTVWV
                     GRPLKIISDGLTDENAEKLQDLKNESSEFGTITRLILRANEQKILLVKEVQTRTEAEQ
                     ALRRSERDYRELFENAHDAILIIDPVQFDVLQANR"
BASE COUNT          720 a          535 c          595 g          731 t
ORIGIN      
        1 ggcgctataa aaaagacggc acgttcgaag gaacacagat acaagctaaa tgcgcttcaa
       61 aatacgaaac ggattacaac gatctcaaaa agggaagtta attcctcaaa gcgtttttca
      121 acaaaagatc attcatcgcg aatgatcttt ttttatttca aggatatggg atgaccaaat
      181 cagcgtacac ggggttattg attttgattc tgtttgtgat ggctgtgtct tgggacgggc
      241 gtcgcgaaaa gatggcgccg ttgtccaaat cggcggcgat cagtattaaa cgcgtaagta
      301 caggtttgtc cacggttggt gcggattcag ccaatatcgg cgcatgggtt gaacaccgtt
      361 tggaaggtag tactaatgcg aatgcattgt atcaggggtt tatcgcgttt cactttgacg
      421 aaatggacac gatgctgacc gtccacagtc ctgcaggccg gaccaatgcc ggtatatttt
      481 cgggagataa acctaccttt agcaagacgg gttatacggc atctctacag acggaccttt
      541 cggaagaaca ttatacggtg tcaacacagc ccattgccaa tttttctaaa ataaaactta
      601 ctcaaatcct caccgcgccc aaaaataacg aacgcatcgt ttatctcgat tttatttttg
      661 aatttgctcc caaagattca ctaaccgatc cgcgtcaata ttctaatttt cgttttctaa
      721 tgggatatga tggcgatatc ggtacgacca ccggcggtat gaataatgat agcacaggtt
      781 attatggcgg taatcaaacc ggtttggcgt atatctacga tgccgtgctt tctttgtaca
      841 gcggggttgc tctgcacgaa aaaggtgtat ccgatagcgc aggcaatttt ggtttatggc
      901 accagacggt taatgcaaat gaattttcta cagtcaagct cgatacattg atgcatgaaa
      961 tgatgatgca tccgtcgtgg aattcgaccg tacaaaatac cgatgcgatg gtgtactgga
     1021 cattgaaccg aggaacgatc gtgccggatg acttcgtgcc atatacagat acgatacgtt
     1081 ttacgttggt caacgggtta tctaagacta ctgtcattga agcctccaaa gggattcatg
     1141 tgccggatgt gttgcctccg gacgaaccgt catcaccctt gccgactact tttgtattgt
     1201 tcaataattc gccgaatccc tttaatccct cgacaacgat acgattttat attcctgaac
     1261 cgatgtttgt acgcctcacg gtttataatt tattaggtca aaaagtgcgc acgctcatag
     1321 cccgtgcagt cgaacccggt ataagccaag tcgtatggga cgcccgtaat gatgttgggc
     1381 aaaccgtcgc cgccggtgta tatctgtata gaatggaaac gccgcaagga attattaccc
     1441 gtcgcatgct gctgctgaaa taaatttcct agcatgaaaa ttcaaaccaa attatcggtt
     1501 ttgctggccg ccgtattgat ttttttagtc agtgcagtgg ccgtttttca gtacgtggat
     1561 ggtcaacgca tcgaacaact ctatcgtcgc gaaataacag agcgaagcat cgtttttgat
     1621 caaatcgttt cgctcaataa taaatctctg gaacttttcg tgtcggatta ttccaattgg
     1681 gatgatatgg tcacgtttat tcataaaccg gattccgtgt gggccgaaag taatattgat
     1741 gcggttttaa gtaaatttaa tgttcaactc ttcttgttgt gtgatcgttc gtttaacgtt
     1801 gtttacacga cacacataaa ttataccgaa aataatgagc tgatacgtac ggtgacccgt
     1861 gattttcaaa aatcagccaa ctccggtttg ttcaaacatt tttatgtttt gataggtgat
     1921 acgatattgg aaatccgcac agcgccggta caaccaagca tggatgtaca tcgcaccaca
     1981 gagcctgcgg ggtatttatt tgtaggacgg gtatgggatg atcgctatgt gaatgaattg
     2041 gccgatgcga cacaaagcga tatgtatata agcacatccg aagcatctct tttaaatgat
     2101 cgcgtgctcc cgagagatac gattgtcgta acgcgcacat ttaagaattt atcggaaaaa
     2161 actatcggat gggtgagttc catacaaaaa gcagaggaat ttaccgagtt ggaccgccag
     2221 gccaatttgc agtttatact gcttgtcgta ttcagtacag ttttatttct gccgctttct
     2281 ttgttgttaa ccgtatgggt cggccgtcct ttaaaaataa tttctgacgg tctgacggat
     2341 gaaaatgcag aaaaactaca agatttgaaa aacgaatcat cagagttcgg cacgatcacg
     2401 cggcttattt tgcgggctaa cgaacaaaaa atattgttag taaaagaagt gcagactcgt
     2461 acggaggcgg agcaagcgct gcgaagatcg gaacgcgatt atcgtgagct ctttgaaaat
     2521 gcgcatgatg ccattctcat cattgatccg gttcagtttg atgtattaca ggccaatcgt
     2581 g
//
LOCUS       DAOQNQ010000397         2568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_23183_length_2568_cov_6.114206, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000397 DAOQNQ010000000
VERSION     DAOQNQ010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23183_length_2568_cov_6.114206"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..1102)
                     /locus_tag="PK754_15475"
     CDS             complement(158..1102)
                     /locus_tag="PK754_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNB10953.1"
                     /translation="MKAFIEMLKLRRYSSNTLGAYEMQLKRLFTHYKDRLPSSLTEHD
                     MLMYIQDVLVPAGVSPSYQNQAINAFKCYFENFLGREKMNLAYQRARVTVRLPVVLSQ
                     DELRGIFASVKNMKHRCILFLIYSSGLRVGEAVNLNVNDIDSRRMLLHIRNGKGGKDR
                     FTILSDQALKLLRDYYKQYRPKKYLFEGVHGGRYSVRSIQQMFTNAASRAGIQKRVSV
                     HSLRHSFATHLLESGVDIRYIQELLGHESSKTTEIYTHVSSRELSKIESPLDRWYTDH
                     PKTGIVREGDPTILNAIAHITRKWRLLRNTHITRLRTL"
     gene            complement(1277..1825)
                     /locus_tag="PK754_15480"
     CDS             complement(1277..1825)
                     /locus_tag="PK754_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10954.1"
                     /translation="MKTTAKLFVLAVLALALSAGSTMAQNPNAEKGKAHKEEKGGKTE
                     KAKKEVKSEDELQPKGKPEGKGSEGNAYGKNKGEMSGKEFGKMRSADAKLKMKEKRKN
                     AHEKITGAEEKIAATEARIKTAKENVEKKKKSGQLKGDKLKEKEEKLKAAEEKIAKLK
                     DLVGKEKDKLTKIPDESAPAAP"
     gene            complement(1847..2056)
                     /locus_tag="PK754_15485"
     CDS             complement(1847..2056)
                     /locus_tag="PK754_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10955.1"
                     /translation="MRKYIMMLAVLALWGIAACGEKKPSAEELDHKEAVRIDSVANDL
                     NASTESLQQQVDETEKAVDDLLKDI"
     gene            complement(2108..>2568)
                     /locus_tag="PK754_15490"
     CDS             complement(2108..>2568)
                     /locus_tag="PK754_15490"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNB10956.1"
                     /translation="RPILTAHRLAEPDYITPALILEDANVHAYYDEVMTRTWEYIARL
                     RAHHVSDENAMYLLPNAVAIRFTESADLQNLHHKMAMRLCYLAQEEIWRASVDETQQI
                     QQVNPVIGNLLLPPCGIRLRAGMRPYCPEGDRFCGERVWTMTLDQYQRVI"
BASE COUNT          636 a          601 c          541 g          790 t
ORIGIN      
        1 aatcggtttt gctacatgga ttgaatctcg accttcagct agagcttcaa attgatgacc
       61 atggatcatt aaaagcatca atgcaaattt cataaaccct cttttgttta aactatcaag
      121 gcagatgtct cataactatt tagtatatga attgcgttta tagggtgcgc agtcgagtaa
      181 tatgcgtatt gcgtaaaagg cgccattttc gggtgatatg cgcaatagcg ttgaggatgg
      241 tgggatcacc ctcccgaacg atacccgtct tagggtggtc ggtgtaccaa cgatccaaag
      301 ggctctctat tttagaaagc tcgcggctgc ttacatgggt ataaatttcg gtggttttgg
      361 aactctcgtg gccaagcaac tcctgtatat aacgtatatc cacccccgat tctaaaagat
      421 gcgtagcaaa actatggcgt aacgaatgca cagatactct tttttgaata ccggcacggg
      481 aagccgcgtt tgtaaacatt tgctggatcg aacgcacact ataccgacca ccatgtacac
      541 cttcaaagag gtattttttg ggccgatact gtttgtaata atctcttaaa agcttcaagg
      601 cctgatcgga taaaatagta aaccgatcct ttccaccttt accattacga atatgaagca
      661 gcatacgcct ggaatcaatg tcgttaacgt tcaggttcac cgcttcaccg acacgtaaac
      721 cggatgaata gatcaaaaaa agaatacagc ggtgtttcat gtttttgacg gaagcaaaaa
      781 taccgcgcaa ctcatcctga ctcagaacaa ccggcaaacg cactgtaacg cgtgcacgct
      841 gatatgccaa attcattttt tcgcgaccga gaaaattttc aaagtaacat ttgaatgcgt
      901 tgatcgcctg attttgatat gacggcgaaa cacccgccgg aaccagcaca tcttgtatat
      961 acataagcat atcgtgttcc gtgagagacg acggcaaccg atctttgtaa tgcgtgaaaa
     1021 gacgcttgag ctgcatttcg taagcaccca aagtattgga actataccgg cgcagcttga
     1081 gcatttcgat gaaagctttc atgtggccac ccagagaatt tattgaagaa ctgatcaatc
     1141 cctgacgaaa cttagcacaa cagtgttttg aggtcaagta aaaaaatgaa aatcgcgacc
     1201 tacgtcagag ataaaataaa aaggcttcat cacatattga tgaagccttt tgtattcaaa
     1261 tcaaatacaa agctaactat ggcgctgccg gagccgattc gtcaggaatt ttagttaatt
     1321 tatctttttc tttaccgaca agatctttga gtttggcgat cttttcttca gcggctttaa
     1381 gtttttcttc cttttctttg agtttatcgc ctttgagttg tccggatttt tttttctttt
     1441 cgacattttc ttttgccgtt ttaatacgcg cttcggtcgc ggcaattttc tcttcggcac
     1501 cggttatttt ttcatgcgca tttttgcgct tttctttcat tttcaatttc gcatcggctg
     1561 aacgcatttt cccgaattcc ttaccactca tttcgccttt atttttcccg tacgcgttgc
     1621 cttcggaccc cttgccttca ggttttcctt tgggctgtag ttcgtcttcg cttttaactt
     1681 ccttctttgc tttttcagtt ttgccgcctt tttcttcttt atgcgcttta cctttttcgg
     1741 cgttgggatt ttgggccatc gtactacccg cgcttaacgc gagtgcgagt acggccaata
     1801 caaataattt tgcagtggtt ttcatatttt tttcctcacg ttgcggttaa atgtctttga
     1861 gtaaatcgtc tacggctttt tccgtttcat ctacctgttg ttgcaggctt tcggtcgaag
     1921 cgttgagatc gtttgcgacg gaatcaatcc ttacggcttc tttgtgatcc aattcctcag
     1981 cggatggttt tttttcaccg catgccgcaa tgccccacaa ggcgagcacc gcaagcatca
     2041 tgatgtactt tctcatacct tactcctcat ttggttagtt ttggagagtc aatccaagta
     2101 gctcaaatca tattacgcgt tgatattgat ctaacgtcat cgtccaaact ctttcgccac
     2161 aaaaacggtc gccctcgggg caatacggac gcatgcccgc gcgaagtcgt ataccgcacg
     2221 gcggtaaaag aagatttcca atcacgggat tgacttgctg gatttgttgc gtttcgtcca
     2281 ccgatgcgcg ccagatttct tcctgcgcca gataacacaa gcgcattgcc attttgtgat
     2341 gcaggttttg cagatctgcg gattccgtaa aacgtatggc taccgcattg ggcaaaagat
     2401 acatcgcgtt ttcgtcggac acatgatgtg cacgcagacg cgcgatatat tcccatgtcc
     2461 gtgtcatgac ctcgtcataa tacgcgtgga cgtttgcgtc ttccaaaatc aacgccggcg
     2521 taatgtaatc cggctcggcc agacgatgtg ccgtgagaat aggacgtg
//
LOCUS       DAOQNQ010000398         2544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_23605_length_2544_cov_4.679389, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000398 DAOQNQ010000000
VERSION     DAOQNQ010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23605_length_2544_cov_4.679389"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1359
                     /gene="tgt"
                     /locus_tag="PK754_15495"
     CDS             148..1359
                     /gene="tgt"
                     /locus_tag="PK754_15495"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNB10957.1"
                     /translation="MKFTLLHKDQSGRHSRARAGILETDHGKIETPIFMPVGTVGDVK
                     AIEHRELKENGAQIILGNTYHLYLRPGTEIIQKAGGLHKFIGWDLPILTDSGGYQVFS
                     LSQLRKLSRDGVKFQSHIDGSKHFFRPEDVVDIQRKLGSDICMVLDECPPYPCSHEYA
                     ARSNRLTIEWAERCKTHFEKSPPLYGHSQTLFPIVQGSVFEDIRLKSAEALVKMDFEG
                     YAIGGVSVGEPKEDVYKIAEMNCAVLPEHKPRYLMGVGTPQDILECIERGVDMFDCVM
                     PTRNARNGTLFTTWGKMTIRNAAYADDFTPIDENCGCYTCRNFTKAYVRHLSSIGEIL
                     GHQLTTIHNIYFYLWLVKEARKHILQDTYTAFKTDLLKRYEENEKNRGSELARHAKTK
                     RKRKDYETSGA"
     gene            1340..1666
                     /locus_tag="PK754_15500"
     CDS             1340..1666
                     /locus_tag="PK754_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB10958.1"
                     /translation="MKLVAPDALKAWIDEGKVMMLLDVREPWEHAIAKLPDSVLIPLG
                     LLNVQSERLTGLDKTTPVVAYCHHGMRSQRACAILESLGFTDVYNLTGGIDYYSDCVD
                     PTIEKY"
     gene            2312..>2544
                     /locus_tag="PK754_15505"
     CDS             2312..>2544
                     /locus_tag="PK754_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10959.1"
                     /translation="MIYLKDVFKNILLSACIVAACLTCDDKDKDNPPKPPVDDIITYE
                     KILGTNASEYVRGGDLTNDGGCIITGDQIIDGD"
BASE COUNT          691 a          513 c          604 g          736 t
ORIGIN      
        1 tctttttatg ttggctatcg aaggtgtcat gcgcagaccg ttgtcgctca aagttaagct
       61 ccgcgcgcaa caagccggca tggctattct gctatcgctg atcgtatata ttttctttaa
      121 tgatatcgtc aaacttacag gtattttttg aaatttacac tgcttcataa agatcagagc
      181 ggccgccatt cgcgtgcacg tgccggtatt ctggaaacgg atcacggtaa aatagagacg
      241 cccattttta tgcctgtcgg tacggtgggc gatgtcaagg ccatcgaaca tcgtgaactg
      301 aaagaaaacg gggcacagat cattctcggt aatacatatc atctgtattt gcgaccgggt
      361 acggagatca tccagaaagc aggcgggctg cataaattta tcggttggga tttgccgata
      421 ttgaccgata gcggggggta ccaagtattc agtctttctc agcttcgtaa actttcccgc
      481 gatggtgtca aatttcagtc gcatattgac ggatcaaaac atttttttcg cccggaagat
      541 gttgtggata ttcagcggaa actcggttcg gatatttgta tggtgctcga cgaatgcccg
      601 ccgtatccgt gttcgcatga atatgcggcc cgttccaatc gcctgaccat cgaatgggct
      661 gaacgctgca aaacacattt tgaaaaatca ccaccgttgt acggtcatag ccaaacactt
      721 tttcctatcg ttcaaggcag cgtgtttgag gatattcgtc tcaaaagcgc agaggctttg
      781 gttaaaatgg attttgaagg gtatgccata ggcggcgtga gtgtcggtga acctaaggaa
      841 gacgtttaca aaatagccga gatgaattgc gccgtattgc cggagcataa gccgcgctat
      901 ctgatgggcg tagggacgcc gcaagatatc ttagaatgta ttgagcgcgg tgtggatatg
      961 tttgactgcg ttatgcctac acgcaatgcg cgcaacggaa ctttgtttac gacatggggt
     1021 aagatgacga ttcgtaatgc cgcttacgcg gatgatttta cgccgattga tgaaaattgc
     1081 ggttgttaca cgtgtcgtaa ttttaccaaa gcctatgtac ggcatctgtc aagcatcggt
     1141 gagattttgg gtcatcaact gacgacgatt cataatattt atttttatct atggctagtc
     1201 aaagaagcac gtaaacacat tttgcaagat acctatacgg ctttcaaaac cgatctattg
     1261 aagcgatacg aagaaaacga aaaaaatagg ggaagcgaat tggcgcgtca tgccaagacc
     1321 aaacgtaagc gtaaagatta tgaaactagt ggcgcctgat gctctgaaag catggattga
     1381 tgagggcaag gtaatgatgt tattggatgt gcgcgaaccg tgggagcatg caatagccaa
     1441 acttcctgat tcggtgctga ttccattggg gcttttaaac gtacaatcgg aaagactgac
     1501 aggtctggat aagacaacac ccgtagttgc gtattgtcac cacggtatgc gaagtcaaag
     1561 ggcatgtgct attcttgaat cgctgggttt taccgatgta tacaacctga ccggtggtat
     1621 tgattattat tcggattgcg ttgatccgac cattgaaaaa tactagttat tctatcttct
     1681 ttttcggcat cttcggtaaa gacttattaa gagtctctta attgtaacaa aaatcccttc
     1741 aatcaaatta ataatgactg atgcagtctt ttatctttca gtgggttccc gctttgcctc
     1801 gactggcgtt cgaacacgtc tcgctttgat acgtcacacc atcggcgtga ctactcagcg
     1861 catgttggcg taactcacgt cgaggtctcg ggacatgtcg cgggaacgac aagacgatgg
     1921 ttttcgtatt gtctcaccct cacacgcggg tgtccactaa aatattacaa attcaatttt
     1981 ttataagata aaacctgact taggtcattg tttttgaatt tttttttagt acgctttcta
     2041 cgtagcgtat ttctattgag ggactttcta tgaatctctc ttcttcgcaa atagcttttc
     2101 caatgtatct ctctgaatga aggcgatgac atatatgaat gaatgtttcc atgctttatt
     2161 acccttgggt gaataaaaaa aggaaagaga atattaccgg attcagacca atgcagattt
     2221 gaattattat ggtactattg attttcccgg tgataatgtt aaacaatacc ccggccttat
     2281 caatcggatt tccggaaagg aggaagcttt tttgatttac ctaaaggatg tttttaagaa
     2341 tattcttctt tcagcgtgta tcgtggccgc atgcctcacc tgcgatgata aagataagga
     2401 taacccgccc aaaccgccgg tggacgatat cataacttat gaaaaaatac tcgggacaaa
     2461 tgcatcggaa tatgtaaggg ggggggatct cacgaatgac ggcggatgta ttattaccgg
     2521 ggatcagatc attgatggag attt
//
LOCUS       DAOQNQ010000399         2540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_23670_length_2540_cov_5.398390, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000399 DAOQNQ010000000
VERSION     DAOQNQ010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23670_length_2540_cov_5.398390"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PK754_15510"
     CDS             complement(<1..486)
                     /locus_tag="PK754_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10960.1"
                     /translation="MIRLFFRLIYFSLAMLGVVVIAAFFLPKHYTVETTIMIKAAPPI
                     VYERAMDLPAWHALAQAAQLPLEKIDGDQLMQLLLQLPSGNGVVGAGVRLDTVRQYIA
                     QGGSALVDKGVNQLKMVCRITETNPPHAFSLRVEGGPFHGLTQSVTLFPTGEGMTTLT
                     MT"
     gene            653..>2540
                     /gene="ppk1"
                     /locus_tag="PK754_15515"
     CDS             653..>2540
                     /gene="ppk1"
                     /locus_tag="PK754_15515"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNB10961.1"
                     /translation="MNEIAKTVSDPVENDSTGIFNPKSFDKSEHFVNRELSWLEFNRR
                     VLEEAQDPAVPLLERLKFLSIFSTNLDEFFMVRVAGVSRQIDANVLHKGADGLLPRDV
                     MDAMSRRIHEIAIIQHDYFFDVLIPQLQKENIAILKPGELSTEQNNFVRDYFQNTVVS
                     ILTPLAIDPSHPFPHLANRALCLVSEVEPKIESKLPKTNLVVVPISLNVIPRFVRLPS
                     AEGTYQFILLEDLIRTCINDLFIGYEVKNCFTIRVTRDSDLTYDEDPSEDLIKTISES
                     LRNRRKGAAVRLQYERGLPDSLLKLLLRELDLQYEDLYPSRGFICLSDLMNLYQQLDI
                     AALKDPLFPPHHIPELETGADMFSIIRSGDILTHHPYQSFEYVTRFVREAAEDPSVVA
                     IKMTLYRIAANSPIAQALQLAAERGKEVAVLVELKARFDEEANIQWARKLEDAGAHVI
                     YGMAGLKTHCKVCLIVRQEKNKIRRYCHLSTGNYNERTAQIYGDLGLFTAHPRFGEDL
                     TNLFNVLTGYSAPPKFHRIKIAPTDLRDTFINKIRREVNHVRDGKPGLLIAKMNALVD
                     KQIILELYRASQAGVQIKCIIRGICCLKPGVKGLSENIEVISIIDRFLEHARIFYFHN
                     NDQPE"
BASE COUNT          702 a          575 c          557 g          706 t
ORIGIN      
        1 tgtcatagtc agcgttgtca tgccttcgcc ggttgggaat aacgtaacgg attgggtcaa
       61 accatgaaaa ggaccgcctt caacacgtag tgaaaatgca tgcggtggat tagtttcggt
      121 aatcctacac accattttaa gttgattgac ccctttgtca acgagcgcag aaccgccttg
      181 tgcaatatat tgccgcactg tatcaagccg cacacccgca cccacaacgc cgtttccgga
      241 tggcaattgc aataacaatt gcatcaattg atccccgtct attttttcca gcggcaattg
      301 agccgcttgc gccagagcat gccaagccgg caaatccatc gctctttcat atacgatcgg
      361 cggagccgcc ttgatcataa ttgttgtttc aacggtataa tgcttcggaa gaaaaaaagc
      421 ggctatgacc accacgccta acattgctaa agaaaaatat atcagacgaa aaaataatcg
      481 tatcatagct gagaagttag aattccatac cgctaaaggc aagcgcattc taaggaatca
      541 caaaaacaca tgtcttatta tttattatga ttatgttaaa cgatgtcttt ccttgcagag
      601 catgcgagga ttacgtaact tattaataca ctgctaaaaa acacgtatcg ttatgaacga
      661 aatagccaaa acggtttccg atcctgtcga aaatgattct acgggaatct ttaaccccaa
      721 atcatttgat aaatctgaac attttgtaaa ccgcgaactg agttggctgg aatttaatcg
      781 gcgcgtactc gaggaagcgc aagacccggc ggtgcctctg ctggaacgcc tcaagttcct
      841 ctctatattt agtacaaatt tagacgagtt ctttatggtt cgcgtggccg gtgtatcgcg
      901 gcagatcgat gcgaatgtgc ttcataaagg agccgacgga cttttgcctc gcgatgtgat
      961 ggacgcgatg tcgcggcgaa ttcatgaaat tgctatcatc caacatgatt attttttcga
     1021 tgtgttgatt cctcaacttc agaaggagaa catcgccata cttaaacccg gcgaattatc
     1081 gaccgaacaa aataattttg tacgcgatta cttccaaaat actgtggttt ccatccttac
     1141 gccgctggcg attgatccga gccacccgtt tccccatttg gccaatcgcg cattgtgttt
     1201 agtatctgaa gtcgaaccca aaattgagag taaattaccg aagaccaacc tcgtcgtcgt
     1261 tcccatttcc ctgaatgtca ttcctcgttt tgtgcgattg ccgtcggcgg aaggaactta
     1321 tcaatttatt ctgttggagg acttgattcg cacgtgtatc aatgatctat tcatcggtta
     1381 tgaagtaaag aattgtttta cgattcgggt gacacgggac tccgatctga cttacgacga
     1441 agatccttcg gaagatctta tcaaaactat ttccgaatct ttgcgtaacc gcagaaaggg
     1501 agcggctgtt cgcttgcaat atgaacgcgg cttaccggat agtttactaa aattgcttct
     1561 ccgtgaactg gatcttcaat acgaggactt gtatccctcc agaggattta tttgtctttc
     1621 cgatttgatg aatttgtatc aacagctaga cattgcggca ctcaaagacc cgctttttcc
     1681 tccccatcat attccggagc ttgaaaccgg tgcggatatg tttagtatta tccgaagcgg
     1741 tgatatactc actcatcatc cgtatcagag ttttgaatat gtcacacggt ttgtgcggga
     1801 agcggcagaa gacccgagtg tagtggcgat taaaatgacg ctttatcgta ttgcagccaa
     1861 ttccccgatc gcgcaagcct tgcagttagc tgccgagcgc ggcaaagaag tcgccgtgtt
     1921 ggtcgagctc aaagcacgtt ttgatgaaga ggccaatatc caatgggcac gcaagcttga
     1981 agacgccggt gcgcatgtga tttatggtat ggccggactc aaaacacatt gtaaagtatg
     2041 ccttatcgtg cgtcaggaaa aaaacaaaat ccgtcgttat tgtcatttat cgacaggaaa
     2101 ctataatgaa cgcacggcgc aaatatacgg tgatctgggc ttatttacgg cgcatccgcg
     2161 ttttggtgaa gacttgacca atttgtttaa tgttttgacc ggatactctg ccccgccaaa
     2221 atttcatcgc atcaaaattg ctccgacgga tttgcgcgat acatttatca ataaaatccg
     2281 acgtgaagtc aatcacgtgc gtgatggtaa acccggcctt ctcattgcca aaatgaatgc
     2341 cttggtggat aaacagatta ttttggaatt gtatcgtgca agccaggccg gtgttcagat
     2401 caaatgcata atacgcggta tttgttgttt gaaacccggc gtaaaaggac tgtcagaaaa
     2461 cattgaagta atcagtatca ttgatcgttt tcttgagcac gcacgtattt tttattttca
     2521 caacaatgac caacctgaat
//
LOCUS       DAOQNQ010000400         2538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_23705_length_2538_cov_5.945228, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000400 DAOQNQ010000000
VERSION     DAOQNQ010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23705_length_2538_cov_5.945228"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2538)
                     /locus_tag="PK754_15520"
     CDS             complement(<1..>2538)
                     /locus_tag="PK754_15520"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10962.1"
                     /translation="AFSNEDAVVPVIEAGNALVLDGVDDYILADSVDLVSTDFTIEFW
                     VKRNAVGNLIISQGALPASTNNFLDIGFEVTSNIYMEFWNNRIQSSYTFDNNWHHIAF
                     TANRSTGERVLYIDGKYETSNFSGIYTGQGKLYIGAESMTLGGPFNGSLDELRIWNHV
                     RSQSQISDQMTKSLRGDEVGLMNLWHFDESDPGTIVYEGSTSANDGVLNNGQGNNFTP
                     SGAMAPVAPAGVYAIAHNGQVELKWNKNTDADFFRYHVYSGITANPTTDIGTTTDGDV
                     NDTTFIATGLVNGQMYYFRVAGEDSAGQIGNYSTQDAAVSVVESGNALALEAGNSEYV
                     ALPSTFENITMTISAWIKPSSAQQHAIWSNRGSSGTNEDGFALGLLPDGTLYYEYGFP
                     SGAVSTSSGQIPINSWTHVALTLDGSIARLYVNGVEQANGAYSRTTSGVNAWIGGYNN
                     GWGTFDGQIDELSIWNYALAPAQITSGLAKPYRGDEVGLVALYHLDEPSASTTALDAS
                     PNAYNGNLVNGADFVTSTAMAPTAVAGISATTFFPDSVRITWNHNSETDLKKYIIYRS
                     TTPGFIPSLTDSIDQTAVGDTAFVDVNAVVGNIYYYRVSAVDSADQAGVYSSDVSADF
                     DPLLVKNTADAGEGSLRAAIAAAASLPGADSIRFAISNATIFIDSDLDINDASGPVVI
                     DGETNNNTVQVSVSFPFGDNTIFSVQSPNVTIRNMTLDAQNGSAQARFGIFFDDAGLA
                     GDNSKAENLTVRNAQRYGIIGQTSNDSVINSTIYNNAWGGVGSTGANWVIRNNRIGTD
                     ATGTIGLGNQDLGVYIFAMATVDSNIIAGNDSTGIYAAGPLNTIRGNR"
BASE COUNT          710 a          644 c          532 g          652 t
ORIGIN      
        1 gcgattaccg cgaatcgtat taaggggacc cgccgcataa ataccggtac tgtcattgcc
       61 ggcgataata ttactatcga cggtcgccat tgcaaaaata tacacaccca gatcttgatt
      121 gcccaatccg atggttccgg tagcatccgt accgatacgg ttattacgta tcacccagtt
      181 tgcaccggtc gaaccaacac cgccccaagc attgttatag atcgtactgt tgatcacgga
      241 gtcattactc gtttgaccga tgataccata acgctgagca ttacgaactg tgagattttc
      301 tgctttactg ttatcacctg ccagacctgc atcatcaaag aaaattccaa aacgcgcctg
      361 tgccgaaccg ttttgtgcat ccagcgtcat atttcgaatc gttacgttcg gtgattgaac
      421 actaaatatt gtgttatcgc caaatgggaa cgacacgctc acctgtaccg tgttgttatt
      481 tgtttcaccg tcaataacaa ccggccccga cgcatcattt atatcaagat ccgaatcaat
      541 gaatattgta gcattggaaa ttgcaaaacg aatactatca gcgccgggta atgatgcggc
      601 ggcagcaatc gctgcgcgca atgatccttc accggcatca gccgtgtttt taacaagtaa
      661 gggatcaaaa tcagcggaaa catcagaaga ataaacgcct gcctgatcgg cactgtccac
      721 agcgcttacg cgataataat aaatattccc gaccactgca ttcacatcca caaacgctgt
      781 atctccgacg gctgtctgat caatcgaatc cgtaagggac ggaataaatc cgggcgtggt
      841 gctccgataa ataatgtatt tcttcaaatc cgtctcagaa ttatgattcc acgttattcg
      901 caccgaatcc gggaaaaacg tcgtagccga aattccagcc acggccgtcg gcgccatcgc
      961 tgtagaagtt acaaaatcag cgccattaac taggttgccg ttgtatgcat tgggtgaggc
     1021 atcaagagct gtagtcgaag cagacggttc atccaaatga taaagcgcta caagccctac
     1081 ttcatcgccg cgatacggtt tggccaaacc ggaagtgatt tgtgccggtg ccagcgcata
     1141 attccaaatt gacaattcat caatctgacc atcaaaggta ccccacccgt tattgtaacc
     1201 gccaatccat gcattaactc ctgaagtggt tcttgaatag gcaccgttgg cctgttcaac
     1261 accattcacg tagagtcggg ctatgcttcc atcgagcgtt aaagccacat gggtccacga
     1321 attgatcgga atctgccccg atgatgtgga tacagcacct gatggaaagc cgtattcata
     1381 gtataatgta ccatccggca aaagccccaa tgcaaagccg tcttcattgg taccggaact
     1441 tccacgatta gaccaaattg cgtgttgttg agccgaagag ggttttatcc aagcggatat
     1501 cgtcatagta atattttcga atgtactggg tagcgcaaca tactcagaat taccagcctc
     1561 aagagccaac gcattgccgg attcaacaac cgaaacggcg gcatcttgag tcgaataatt
     1621 tccaatttgt cccgcactgt cctcacccgc aacacggaag tagtacatct gtccgtttac
     1681 taaacccgtt gcaataaagg tagtgtcgtt gacatcacca tctgtcgttg taccgatatc
     1741 cgttgttggg ttagccgtga tgccgctata aacatggtaa cggaagaaat cggcatctgt
     1801 atttttgttc cacttgagtt caacttgacc attatgcgca atcgcgtaaa ccccggcagg
     1861 cgctacagga gccatggcgc cggacggcgt gaaattatta ccttgaccat tgttaagtac
     1921 gccatcattc gcagaagttg aaccttcata cacaattgtt cccggatcac tttcatcaaa
     1981 atgccacagg ttcattaacc cgacctcatc tccacgaagc gactttgtca tttgatcact
     2041 gatttgcgat tgcgaacgaa catgattcca aatacgcaat tcgtctaagc ttccgttaaa
     2101 aggtccaccc agcgtcattg actcggcacc gatgtacagt ttaccctggc ccgtatatat
     2161 accggagaag tttgatgttt cgtatttacc gtcaatatac aaaacacgtt cgccggtact
     2221 tcgattggct gtaaatgcga tatggtgcca attattatcg aatgtgtatg aactctgaat
     2281 acgattgttc cagaattcca tataaatatt actagtaact tcaaaaccga tatcgaggaa
     2341 attattcgta ctagccggca aagcgccttg agaaatgatc aggtttccga cggcattacg
     2401 tttgacccaa aattctatag tgaagtcggt tgaaaccaaa tctaccgagt cagccaagat
     2461 ataatcgtca acgccatcca ataccaacgc atttccggct tctataaccg gcacgaccgc
     2521 atcttcattg gagaatgc
//
LOCUS       DAOQNQ010000401         2533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_23784_length_2533_cov_6.881759, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000401 DAOQNQ010000000
VERSION     DAOQNQ010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23784_length_2533_cov_6.881759"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PK754_15525"
     CDS             complement(<1..182)
                     /locus_tag="PK754_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10963.1"
                     /translation="MNYRVILVSVMTLIMTSVFILSVKPEAKAVEVSAESILIENFQT
                     STTGQFPKGWGWLDGTS"
     gene            complement(202..846)
                     /locus_tag="PK754_15530"
     CDS             complement(202..846)
                     /locus_tag="PK754_15530"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HNB10964.1"
                     /translation="MEDAVVKPYYVVDVKRKSTTFEVAAMAKIRNQFCERYPALAERF
                     LAVEMIHKDEIKPNSEVTEYFQLLASKTRIGSQYDWLARFAHQFQIDALELCIEKFDS
                     DGSRIFGDYIVPLLKGVGHECRVEGPFTEPGMRLFTCFRFPIIHIEKNEMRRIAEKQG
                     FVDLMRLIWFCHHPAKDGKPCGKCRPCQLANGSGMTYEFSKVSLLEKTINFFKS"
     gene            complement(930..2282)
                     /locus_tag="PK754_15535"
     CDS             complement(930..2282)
                     /locus_tag="PK754_15535"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNB10965.1"
                     /translation="MIGRTLVAMSKSGMKYVTIICRMSDRTLIERCVKEVDAERISVS
                     PTIYSMDDDVNLSKAVNEITKSSESTWMLMTYNKIIHPTFILPARECVQTPALITYNG
                     VMRQGDKVIFGEEMAPKFQTQFQDVSIFEMVSVQDNKVKTWNTADAPHFSAGILVVRN
                     TDIVLAETRQSISELIKFFIKKELLKTVSVSNGWCMPVNKSIASSVIKNVFWKMAFKE
                     ISGEFSKAVNSKLSKPLTFWFSAHQYKPDTISNMSLVLFIVASGFLLIPEYWAMVVFA
                     VVWQFSAGVLDRCDGEVARMRNHESEAGGKFDMIVDDLRFALPLMALGAACFLELPDV
                     RYVIVAAITLAGSFAITFFEQATMRRFGYVSRQKLNVDYLKAKGDTSRFSATMKKLQP
                     VFKGDIRTFYVFLLTFLGEKTIIFWVLVFYHVFVSAVALFGALTFPQLIKKIQSETKR
                     "
BASE COUNT          757 a          597 c          457 g          722 t
ORIGIN      
        1 gacgttccgt ccaaccatcc ccaacctttc ggaaattgac cggtagtaga agtttgaaag
       61 ttctcaatca gaatagattc ggcagaaact tctacggctt tggcttccgg tttgacgctc
      121 aatataaaaa ccgatgtcat tatcaaagtc ataacagaaa ctaatataac acgatagttc
      181 ataacatccc tctttcgtat attacgactt aaaaaaatta atcgtttttt ccaataaaga
      241 taccttcgaa aactcgtacg tcataccgga accgttggca agctggcaag ggcggcactt
      301 cccgcacggt ttaccgtctt ttgctgggtg atggcaaaac caaatcagcc gcattaagtc
      361 aacaaatccc tgtttttcgg ctattcgccg catttcattt ttctctatgt gaataatggg
      421 aaacctaaaa caagtaaaca gcctcatacc gggctccgta aacggtcctt caactcggca
      481 ttcatggcca acgcctttca gcagtggaac gatatagtcg ccaaatattc gtgatccatc
      541 agaatcgaat ttttcaatac aaagctcaag tgcgtcaatc tgaaattgat gcgcaaaacg
      601 agcgagccaa tcatactgag aaccgattct cgttttggat gcgagaagtt ggaagtattc
      661 ggtcacttcg ctattaggtt tgatctcgtc tttatgaatc atttctacag caagaaatct
      721 ttcggccaat gctggatagc gttcacaaaa ttgatttcga attttcgcca tcgccgccac
      781 ttcaaaggtg gtggatttcc gtttaacatc aactacataa tagggtttca caaccgcatc
      841 ttccatcagt agcgcttcca aaacacgaaa actggaatcc catccgccgg tccaaagcac
      901 atgtatagtt ttcatatttt ccatccagtt tagcgtttgg tctctgattg gattttttta
      961 atcaactgcg gaaacgtcaa cgcaccgaat aaagcaactg cgcttacaaa tacatgatag
     1021 aacactaata cccaaaaaat aatcgttttc tcgccgagaa aagtcaacaa aaacacataa
     1081 aaagtacgaa tatcgccttt gaatacgggt tggagttttt tcattgtggc gctgaagcga
     1141 ctggtatcgc ctttggcttt cagataatct acgttcagtt tttggcgact cacataacca
     1201 aaacggcgca ttgtagcttg ttcaaagaat gtaatagcaa aactgcccgc gagcgtgatc
     1261 gcggctacta tgacgtatcg cacatccggc aattccaaga aacacgccgc acctaacgcc
     1321 atcaaaggta gagcaaatcg taagtcatcc acgatcatat caaattttcc gccggcctcg
     1381 gattcatgat tacgcatacg tgctacttcg ccatcgcaac gatccagcac gccggccgaa
     1441 aactgccaaa ctacggcaaa aacgaccata gcccagtatt ccggtattaa taaaaacccg
     1501 gacgctacaa taaaaagcac gagcgacata ttactgatcg tatccggttt gtactggtgt
     1561 gcgctaaacc aaaatgtaag cggtttggat agtttactat tcacagcttt agaaaattct
     1621 ccgctgattt ctttaaaagc cattttccaa aaaacgtttt ttattaccga cgacgcaata
     1681 gatttattga ccggcataca ccaaccgttg ctgacagaaa ccgttttcaa caattctttc
     1741 tttataaaaa actttatgag ttcagatata ctttgccgag tttctgctaa aacaatatcc
     1801 gtgttacgaa cgactaaaat tccggctgag aagtgcggcg cgtcggccgt attccatgtt
     1861 ttcactttat tatcttgtac tgataccatt tcaaaaatcg aaacatcctg aaattgggtt
     1921 tgaaacttgg gcgccatctc ttcgccaaaa ataaccttat ccccttgacg catgacgcca
     1981 ttatacgtga tcaatgccgg cgtttgtacg cattcgcgtg ccggtaaaat aaaagtcgga
     2041 tggatgattt tattatatgt catcagcatc catgtgcttt ctgatgattt tgtaatctca
     2101 ttaacggctt tacttaaatt tacgtcatca tccatactat agatcgtagg agatacactg
     2161 atacgttcgg catccacttc tttaacacaa cgttcaatga gtgtacgatc cgacattcgg
     2221 catattatcg ttacatattt catgccggat ttactcatcg ccaccagagt tcgtccgatc
     2281 aacgtctctc caccgtacaa cgtatcaaaa tccggcgcgt cgtttgttgg ttttattgca
     2341 attattatcg cgttttgaat catcactcaa caggtttaaa ctatcttatt aatttctttt
     2401 ttgtagcaat aatcacacag tgtatttatt cggccacagt ttgaggttcg tccgtagcta
     2461 aaatccatcc gcaacggaac aaatgaaaca taaaactgat gaccatccaa atacctaaaa
     2521 aataaaacgc ttc
//
LOCUS       DAOQNQ010000402         2524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_23948_length_2524_cov_4.230458, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000402 DAOQNQ010000000
VERSION     DAOQNQ010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23948_length_2524_cov_4.230458"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PK754_15540"
     CDS             <1..370
                     /locus_tag="PK754_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10966.1"
                     /translation="NLSQTIDDSNIDMKNILSDLQTALSSLIESARADSHRVMESVQT
                     LNEAVIWMERNQNEMNDALETAATISKMFSDNHAAVMAHTENIKRIQHSIQTTNAVFN
                     SLKNHIHDLKAGYQSIGSLS"
     gene            381..2072
                     /locus_tag="PK754_15545"
     CDS             381..2072
                     /locus_tag="PK754_15545"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB10967.1"
                     /translation="MKFASKLFLVFVGFSAFLSLAFVGTFYLFVFKKAELDLKSQAQL
                     HAYSLAVTLSKLIDGDKHQTILTRADENSEAYKEIQRVLRTFRDANREVGMPVQYVYT
                     KIRTEHKDTLAYVVDAQEDDRSIASIDPTTKDTTFNFTPVGQRVEVTWQREFQKASVS
                     DFYSDPWGMWITGAGPIRNSAGTVVAFAYVDVSGTKVLEELNAIKQRITVVSVIALIA
                     LLTLSAILSYVLANFVNRPLNNLREGLTRIKDGVFDFRIPINRKDDFQDLAEAFNVMS
                     ENMRTMAAQISNGSRKIASTSTEILAISREQATTSSQQSISVTETTATMEELTSTSRY
                     IAENSESVVKIAAETHEISQEAVDLSGVAKDKMEEIRRKSDQDTSAIADLNKKMQKIT
                     EVIEIINTVTEQTKLISFNARLEATGAGEAGRRFSVVASEIRRLAENVAESTEEIKET
                     VHEIRVAMDSLVKNSKESAYKIREGVDLVSKVSTVLENILLAAQNTTESAKQISLSTQ
                     QQRTASEQVVTTLKEISEGAKHFVKSSNQAAIISGDLNALSDELNKTLNNFKVGR"
     gene            2076..>2524
                     /locus_tag="PK754_15550"
     CDS             2076..>2524
                     /locus_tag="PK754_15550"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HNB10968.1"
                     /translation="MRKLVLWDIDGTLIRCGKSPRNAIVAAMEEVFGTRGDVDNYPFS
                     GKTDPQIIYDVMTAAQMDEQHVLANMHVALAGYVARLEKTLMPDEIRIFEGIVDLLNQ
                     LSGHNEVIQGLLTGNVIEGARIKLSRAGLGHYFFNGKPPLGAFGSDAR"
BASE COUNT          723 a          568 c          614 g          619 t
ORIGIN      
        1 taacctctcg cagaccattg atgactcaaa tatagacatg aaaaatattt tgagtgattt
       61 gcaaacggca ttgtcctcgt tgatcgaatc ggcgcgtgca gattcgcacc gcgtcatgga
      121 atcggtccaa acactgaatg aagccgtgat atggatggag cgtaatcaaa acgagatgaa
      181 cgatgctctc gagacggctg cgacgatttc taaaatgttc tccgataatc atgctgcggt
      241 tatggcgcac acggaaaata tcaaacgcat tcagcattct atccagacca caaacgccgt
      301 atttaattct ttaaaaaatc atatacacga tctcaaggcc ggttatcaat ccatcggatc
      361 cttaagctga cggagcttgt atgaaatttg catccaaact ttttctggta tttgtcgggt
      421 tttcggcttt tctgagcctg gcatttgtag gaacgttcta tttatttgtt tttaagaaag
      481 ccgaactgga cctgaaatcg caagcgcagt tgcatgcgta ttcactggcc gtaacgttga
      541 gcaagcttat tgacggggat aagcaccaaa ccatacttac ccgagcggac gaaaattcgg
      601 aagcctataa ggaaatacag cgtgtactgc ggacatttcg cgatgccaat agagaagtcg
      661 gtatgccggt acaatatgtg tataccaaaa ttcgcacgga acacaaagat actttggcgt
      721 atgtcgtaga tgcacaggaa gacgatcgtt ccatagccag cattgatccg acgaccaagg
      781 atacgacgtt caattttacg cccgtagggc agcgtgtcga agtgacgtgg cagcgcgaat
      841 ttcaaaaagc ttccgtaagc gatttttact ccgatccctg ggggatgtgg attacagggg
      901 cggggcctat tcgaaacagc gccggtacgg ttgttgcttt tgcctatgtg gacgtcagcg
      961 gtacgaaagt tttggaggaa ctcaatgcga tcaagcaacg catcaccgtg gtttctgtta
     1021 tcgcattgat agcgctgttg acgctttcgg cgattttgag ttacgtattg gctaattttg
     1081 tgaatcgccc gttaaacaat ctgcgggagg gacttacgcg catcaaagat ggcgtgtttg
     1141 attttcgaat accgatcaat cgcaaagatg attttcagga tttggcggaa gcctttaacg
     1201 tgatgtctga aaatatgcgt acgatggccg ctcagatatc caacggttca cgtaaaatag
     1261 cttcgacgtc cacagaaata cttgccattt cacgcgagca agcgacgacg tccagtcagc
     1321 aatctatttc cgtaaccgaa accaccgcga cgatggagga actcacttcg acatcgcgtt
     1381 atatcgcgga gaattcggaa tccgtagtaa aaatcgcggc ggaaacgcac gaaatttctc
     1441 aggaagccgt tgatctttcc ggtgtggcaa aagataaaat ggaagaaatt cgccgtaaga
     1501 gcgatcagga caccagcgcg attgccgatc tcaataaaaa aatgcagaaa atcaccgaag
     1561 ttattgagat catcaatacg gtcaccgaac agaccaaact tatttcgttt aatgcgcgcc
     1621 tggaagcaac cggggctggc gaagcgggac ggcggttttc cgtcgtggcg tcggaaattc
     1681 gccgtctggc cgaaaacgtt gccgaatcca ccgaagaaat aaaagaaacc gtacatgaaa
     1741 ttcgtgtggc tatggattcg ttggttaaaa actccaaaga aagcgcatac aaaatccgtg
     1801 aaggtgtgga tctggtatcc aaagtctcca ccgtactgga aaacatcctt ctagcggcac
     1861 aaaacactac cgaatcggcc aaacaaattt ccctgtccac gcaacagcaa cgtacggcga
     1921 gcgaacaggt ggtcactaca ttaaaagaaa tctccgaagg cgcaaaacat tttgtcaaat
     1981 caagcaatca ggccgccatc atatccggcg atctcaatgc gctctcggat gaactcaata
     2041 aaacgctcaa taatttcaaa gtaggccgtt aaacgatgcg caaactggta ttatgggaca
     2101 ttgacggtac attgattcgc tgcggtaaat cgccgcgtaa tgccatcgta gccgcgatgg
     2161 aagaagtttt cggaacgcgc ggtgatgtgg ataattatcc tttttcagga aaaaccgatc
     2221 ctcagatcat atatgacgtg atgacggccg ctcagatgga tgaacagcat gtgctcgcaa
     2281 acatgcacgt tgccctcgca ggttatgttg cgcgtctcga aaaaacactg atgcctgatg
     2341 aaattcgtat ttttgagggc attgttgatc ttctgaatca actatctgga cataatgaag
     2401 tcattcaagg tctattgacc ggtaatgtaa tcgaaggtgc gcgcatcaaa ctgagccgag
     2461 ccgggttggg gcattatttt tttaacggta aacctccgct tggagcattt ggcagcgatg
     2521 cacg
//
LOCUS       DAOQNQ010000403         2520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_24021_length_2520_cov_4.027181, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000403 DAOQNQ010000000
VERSION     DAOQNQ010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24021_length_2520_cov_4.027181"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="PK754_15555"
     CDS             complement(<1..1134)
                     /locus_tag="PK754_15555"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HNB10969.1"
                     /translation="MVILGISTGHDAGACVMIDGKIISAINEERLNRKKMYWGFPTLS
                     IPECLRLAQIQPGAIQAVAIAGTSSTRAKPMEFGYKSVGGFRRIFAELSRTPLTGLLM
                     GTEIGTKLTRKVFESSFFRGTSEIDSALSAAGIHAPIHFIDHHLSHATSAYYTSGWND
                     CVTITLDASGDGWCSRVYDCENGTMKLINSIPAYHSPGFYYCYVTHLLGFIALRHEGK
                     VTGLAAFGKSDQTAAIFSERIAYDSNKFRFDNRGGWLQGEMAHLQKQLEGFSKEDIAA
                     GIQKHLEDMVTAYAKDVVRKTGRKRIALSGGVFSNVKLNQDVWKSTGVEEVYVFPNMG
                     DGGLATGAAFEVFRQKKGTCLPYRLHDVYLGTSYNDSEIETALR"
     gene            complement(1115..2305)
                     /locus_tag="PK754_15560"
     CDS             complement(1115..2305)
                     /locus_tag="PK754_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-aminolevulinic acid dehydratase"
                     /protein_id="HNB10970.1"
                     /translation="MDLTTTRDAFQKLERYIQQRDYSGYDCYDALNSPFLSFITKPHK
                     YLRIAAIQFIKKCPINLRPVLFVKPGHNPKGLGLFLSATAMAYRATDDKNYLKSLEHL
                     TTLLRQNESQGYSGSCWGYNFDWQSRVFFVPKYTPTIVNTSFVAQAFVDAFIATGREE
                     FLVKARSACDFIIRDLYHTREGDRLSFSYTPIDRLKVHNANILGAGLLARVYAHTKEK
                     ELYDYAKASAEYVLHYQRPDGSWYYAEETIQHWIDSFHTGFVLDSLDWYVRGTGDQTP
                     VNAIKTGFRYYIENFFLEDGTPKYYHDRIYPIDAHSPAQAFVTLTRLGGLDQRASNIR
                     DHVRDWTLLHMRSPKGYFYFQKHPNYINKIPYMRWVQAWSYYGLAHLLFYDTFKEIHG
                     YSRY"
     gene            complement(2333..>2520)
                     /locus_tag="PK754_15565"
     CDS             complement(2333..>2520)
                     /locus_tag="PK754_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10971.1"
                     /translation="GSIQSGQDAPIPLRESVNAMIATFKILESLQLGRPVDIVLDDRQ
                     DFLSGLSRIYTEHLKTD"
BASE COUNT          702 a          581 c          586 g          651 t
ORIGIN      
        1 ccggagcgcc gtttctattt cactgtcgtt gtacgaagtg cccaaataca catcatgcaa
       61 acgatagggt aggcacgttc cttttttctg acgaaatacc tcaaaagccg cgcccgtagc
      121 caatccgccg tctcccatgt tagggaaaac ataaacttct tcgaccccgg tggatttcca
      181 tacatcttgg ttgagcttga cattagaaaa tacaccgccg ctcaatgcga tgcgcttacg
      241 gccggtttta cgtacaacgt cttttgcgta ggccgtaacc atatcttcaa gatgtttttg
      301 gattcctgcg gcaatgtctt ctttactgaa tccttcgagc tgtttttgaa gatgcgccat
      361 ttcaccttgg agccatccgc cacggttatc aaaacgaaat ttgtttgaat cataggcgat
      421 acgttcacta aagatagcgg ccgtttgatc agacttgcca aatgcggcga gcccggtaac
      481 cttgccttcg tgacgcagtg cgataaaacc gagcaaatgg gtcacatagc aataatagaa
      541 acccggtgaa tgataagcag gaatggagtt gatcagtttc atcgtaccgt tttcacaatc
      601 gtacacgcgc gaacaccaac cgtcaccgct ggcatccagt gtgatagtca cgcagtcatt
      661 ccagcccgac gtataataag ccgatgtcgc atgggataaa tggtgatcaa taaaatgaat
      721 gggtgcgtga atacccgccg cgctgagagc cgaatcaatt tcagaagtgc cgcggaaaaa
      781 agaagattcg aatacttttc gcgtcagttt agtgccgatt tccgttccca tcaaaagccc
      841 tgtcaggggt gtacggctga gttcggcaaa aatacgacga aaaccaccga cggatttgta
      901 accaaactcc ataggtttgg cacgtgtgga actggtaccg gcgatcgcta cggcttgaat
      961 agcacccggc tgtatttgcg ccaaacgcaa acattcgggg attgaaagcg tcggaaagcc
     1021 ccaatacatt ttttttcgat tgagacgttc ttcattgatt gcagaaatga tcttgccgtc
     1081 aatcattaca caagctccgg catcatgacc ggtgctaata ccgagaataa ccatgaatct
     1141 ccttaaacgt atcgtaaaat agcagatgcg ctaaaccgta atatgaccag gcttgaaccc
     1201 atcgcatata aggtatcttg tttatataat tcggatgctt ttgaaagtaa aaatatcctt
     1261 tcggtgaacg catatggagc aacgtccaat cgcgaacatg atcccgaata tttgaagccc
     1321 gctgatccag tccgcctaaa cgcgtaagcg tcacaaaagc ttgagccgga ctgtgcgcgt
     1381 cgatcggata tattctgtcg tgataatatt tgggtgtccc gtcctctaaa aagaaattct
     1441 ctatgtaata acgaaaaccg gtttttatcg cattcacagg tgtttggtcg cctgttccgc
     1501 ggacatacca atccagagaa tcaagcacaa acccggtatg aaaactatca atccaatgct
     1561 ggatcgtttc ttcggcataa taccaggagc cgtcggggcg ctgataatgt aaaacatatt
     1621 cggcgctggc tttggcataa tcatataatt ctttttcttt ggtatgggca taaacccttg
     1681 ccagaagacc cgcaccgaga atgttggcgt tatgtacttt gagccgatct atcggtgtat
     1741 agctaaacga aagtcggtca ccctcccggg tatgatacag atcgcgtatg atgaaatcac
     1801 acgctgaacg cgccttcact aaaaactctt cgcggcctgt cgcaataaac gcatcaacga
     1861 aagcctgagc gacaaacgac gtattgacga tggtcggcgt atatttgggt acaaagaaca
     1921 cacggctttg ccagtcaaaa ttataacccc aacaactgcc cgaataaccc tgactttcat
     1981 tttggcggag caaagtagtc aaatgttcca gcgatttcaa ataattttta tcatcggttg
     2041 cgcggtaagc cattgccgtg gccgacaaga aaagtcccag gcctttgggg ttgtggccgg
     2101 gctttacaaa taaaacaggg cgaagattga ttgggcattt tttgatgaac tgaatcgcag
     2161 caatacgaag atatttatgt ggctttgtaa taaaacttag gaacggagag ttcaacgcat
     2221 catagcaatc atatccgcta tagtcacgtt gttgtatgta tcgttccagt ttttgaaatg
     2281 cgtcacgtgt ggtggtgaga tccataaatg ctgttataca gggggagatt gattagtctg
     2341 tttttaggtg ttcggtgtag atgcgagata acccggaaag aaaatcctga cggtcatcca
     2401 acacaatatc aaccgggcga ccgagttgaa gcgattcgag aattttgaac gtggcaatca
     2461 tcgcattgac ggattcgcgt aaggggatcg gagcgtcttg accggactga attgatccga
//
LOCUS       DAOQNQ010000404         2510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_24219_length_2510_cov_5.331976, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000404 DAOQNQ010000000
VERSION     DAOQNQ010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24219_length_2510_cov_5.331976"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="PK754_15570"
     CDS             complement(<1..827)
                     /locus_tag="PK754_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNB10972.1"
                     /translation="MSIINEIKKRRTFAIISHPDAGKTTLTEKFLLYGGAVQLAGSVT
                     ARKNQRTSRSDWMELEKQRGISISSTVLQFEYTGFKINLLDTPGHQDFSEDTYRVLTA
                     VDAVVMVIDAAKGIEAQTRKLFEVCRRRRVPIFTFINKLDRPTLEPLALIDEIESILQ
                     IHVYPMNWPLGTGVDFRGVLDRQAKEVHLFERTTGGAYRAPVSVFDVSDPLVKNMMSP
                     NTYATVVEELEMLDAAGASFDVKAVHRGELTPVFFGSAMNNFGVQLLLDGFLRYSIEP
                     "
     gene            954..2198
                     /locus_tag="PK754_15575"
     CDS             954..2198
                     /locus_tag="PK754_15575"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HNB10973.1"
                     /translation="MILRNILSTILLIIIFSLAGCQANHTTAQSDAGKSSVILISLDG
                     FRYDYLEKYTTPNLTALAKRGVRSEALISVFPSKTFANHYSIITGLYPAHHGIISNQF
                     WDPEYQKAFSYKDSNANKDSHWWGGEPLWNTVEKQNIKAASFFWPGSEALIQGHRASL
                     WKRYNGAVRNYDRIDTVLSWLTLPQNLKPRFIATYFSITDEVGHEFGPESQEMRRAVM
                     HVDSVIGSLVEGIDRMGLSEQVNIILVSDHGMATVLPDSLIFLDDMIDVNSVRILNET
                     PFVGIWTKSGNVDEVYKTLVNRHPRMQVYTRENVPARFKFKGNPRIPEIICLTEPPWS
                     ITTRDYARRYKDRWLYGSHGFDPEIKEMGGLFIASGPAFEKGKMIPSFENIHIYTLVC
                     HILGISPSANDGDINVFKSAIK"
     gene            complement(2267..>2510)
                     /locus_tag="PK754_15580"
     CDS             complement(2267..>2510)
                     /locus_tag="PK754_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HNB10974.1"
                     /translation="RDSILAAPIVLDLALFLDLASRSGFKGIQEWLSFYFKSPQHATA
                     VYPEHDLFIQLMKLKNTLRWMRGEELITHLGLEYYD"
BASE COUNT          706 a          519 c          565 g          720 t
ORIGIN      
        1 ggttcgatgg agtagcgaag aaatccgtcc aatagcagtt gcacaccaaa attattcatt
       61 gcactgccaa aaaaaacggg ggtgagttca cctcgatgaa ccgcttttac atcaaaggaa
      121 gcaccggccg catccagcat ctccaactct tcgactacag tcgcgtaagt attgggactc
      181 atcatgtttt tgacaagcgg gtcgctgaca tcaaatacgg aaaccggtgc acgatacgcg
      241 ccgccggttg tgcgttcaaa aagatgtact tctttggctt gacgatctaa aacgccgcga
      301 aaatccacgc cggttcccaa tggccaattc atcggataca cgtggatttg caaaatactt
      361 tcgatttcat cgatcagtgc taaaggctca agtgtagggc gatccaattt attgataaac
      421 gtgaaaatcg gtacgcgccg ccgccggcac acttcaaaaa gtttacgcgt ttgtgcttca
      481 atacctttgg ctgcgtcaat caccataacc acggcatcca cggctgttaa tacacgataa
      541 gtatcctcgg aaaaatcttg atggccgggc gtatcaagta agttgatctt gaaacctgta
      601 tattcaaatt gtaaaacagt agaggatatc gaaataccgc gttgcttttc gagttccatc
      661 caatcagaac gagacgtgcg ttgattttta cgcgccgtaa ccgatccggc cagttgtaca
      721 gcgccgccat ataacaaaaa tttctccgtc agggttgtct taccggcgtc cgggtgagaa
      781 ataattgcga acgtgcgtct tttttttatt tcgttgatta tgctcatggt tttccttcaa
      841 ttcggcgcgc atattagaca aaaaagcaac aacctgcaat taaatatttt cttttatttg
      901 tgcattgagt atgttagtcg catctctgta aaatccttat tgggagtgct tttgtgatac
      961 tacgcaacat tttatcaact attcttttaa tcatcatctt tagcttggcg ggatgccaag
     1021 ctaatcatac taccgcgcaa tccgacgcag gcaaatcatc cgttattttg atttcattgg
     1081 atggatttcg ttatgattat ttggaaaaat atacgactcc aaatttgacg gctttggcca
     1141 aacgcggcgt ccgttccgag gcgttaatat ccgtttttcc gtctaaaact ttcgctaatc
     1201 attattcgat aattacgggt ttatatccgg cgcatcatgg gataatttcg aatcaattct
     1261 gggatccgga atatcaaaag gccttttcct ataaagattc caacgccaat aaagattcgc
     1321 attggtgggg tggagaacct ttgtggaata cggtagaaaa acaaaacatc aaagccgcat
     1381 cttttttttg gccgggatcc gaagcgctaa ttcaaggtca tcgtgccagt ttgtggaagc
     1441 gatataacgg tgctgtgcga aactatgacc gaattgatac tgtgctttcc tggcttacgt
     1501 tgcctcaaaa cttgaaacct cgatttattg ctacctattt tagtattacc gatgaagtcg
     1561 gacatgaatt tggtccggaa tctcaagaga tgcgccgagc cgtaatgcat gttgattccg
     1621 tcatcggttc gttggtcgaa ggcattgatc gaatgggatt gagtgaacag gtaaatataa
     1681 ttttggtgtc cgatcatggt atggctactg ttttgccgga tagtcttata tttctcgatg
     1741 atatgataga tgttaactcc gtacgcattt taaatgaaac tccgtttgtg ggcatatgga
     1801 caaaaagtgg aaatgtggat gaggtttata aaaccttggt caatagacac ccgcggatgc
     1861 aggtttatac acgggaaaac gttccggcgc gttttaaatt taaaggaaat ccgagaattc
     1921 ctgaaatcat atgtctgacg gagccgccgt ggtctatcac aacgcgtgat tatgcaagac
     1981 ggtataaaga ccgatggctt tatggttcac atggttttga tccggaaatc aaagagatgg
     2041 gcggcttatt tatcgcgtca ggcccagcgt ttgaaaaagg aaagatgatc ccatcgtttg
     2101 aaaacattca tatctataca ttggtgtgtc atatacttgg aatatcgcct tcggccaatg
     2161 acggcgatat caacgtattt aagtcggcga taaagtgaaa tctcagacga tagaaataaa
     2221 aaagggagcg ctaaacgctc ccttttttga tgttaacaat cttcgattaa tcgtaatatt
     2281 ccaatcccaa atgcgtgatg agttcttcgc cgcgcatcca acgcaatgtg tttttgagtt
     2341 tcatcagttg gataaagaga tcatgttctg gataaactgc cgtggcgtgt tgagggcttt
     2401 taaaataaaa cgaaagccat tcttgaatac ctttgaatcc ggagcgggat gccagatcaa
     2461 ggaataaagc aagatctaag acgatgggtg cagctaatat ggagtcacgg
//
LOCUS       DAOQNQ010000405         2507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_24287_length_2507_cov_2.718189, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000405 DAOQNQ010000000
VERSION     DAOQNQ010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24287_length_2507_cov_2.718189"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PK754_15585"
     CDS             complement(<1..362)
                     /locus_tag="PK754_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10975.1"
                     /translation="MKWLKYGMIFLAASVSLFAKDDLDDVIENASKRVIRRLRDRMKD
                     PTTPVRIGVFNFERLDTYESLTYHVFENYVNDQIVLTLMKNRGKLQFEVVERNSLEKV
                     IREQDFGASDYVNQDQSV"
     gene            complement(395..1072)
                     /locus_tag="PK754_15590"
     CDS             complement(395..1072)
                     /locus_tag="PK754_15590"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HNB10976.1"
                     /translation="MSNPLLWVWEAVQKKAARVLAERLNRDLISYHLRTRNNMENLKR
                     ALKPGDVILVEGNKLISRVIMAVTHSTWSHCALYVGDGKIIDPLPSTGTVVNPVEALA
                     HLNIRVCRPFGLSGEAIDKVVSFAMSHVGRGYDHINISNLLFSFFKKKVDYTEFLGDI
                     SGSNEVCSGLIAEAYNQVGFPVLSGVNFSQIVPGDYDLSPNFEIIKFNKIYFNKNESL
                     VAREWHG"
     gene            1238..2359
                     /locus_tag="PK754_15595"
     CDS             1238..2359
                     /locus_tag="PK754_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10977.1"
                     /translation="MSQVRRYSLMISVFLLFGYALTAQSKRPITADDLWAFKRLSGFT
                     VSPDNKYGCFVVTDYNVKENKGNSDLYLIELATGKTKRLTTNVGSDNSPAWHPNSRTI
                     AFISKREGDYAQLHQIDIDGGEASLITEMPMGAVAPKYFPDGKRIAFVSTILPAYEND
                     FDGMKKNLKERKDSKMSAKVSENRFFRYWDTWLTDGYVPHLYSFHIETKERKDLTPGL
                     NELVSSDGSLDYDISPDGQWITFTRNSSNPPYAQLNSDVFLFKAGEYASAVNITADNL
                     DDDGGAVFSKDGKSLWYSRSFEKNHPDLSHVVRYDLTSKEHKVITDKMDRSFGSYTES
                     DNKKDWYFVAEDMGRTSVYNYNTQNQKIEKIFTEAAWPM"
BASE COUNT          745 a          578 c          502 g          682 t
ORIGIN      
        1 tgcaccgatt gatcttgatt cacataatcc gatgcgccga aatcctgttc gcgaatcact
       61 ttttcgagcg aattgcgttc gaccacttca aactgtagtt ttccccgatt tttcatcagc
      121 gtcaaaacaa tctgatcgtt cacataattt tcaaaaacat gatacgtcaa gctttcatac
      181 gtatcaagtc gctcaaaatt aaagacaccg atacgcaccg gcgtcgtggg atctttcatg
      241 cgatcacgaa gcctgcgaat aacccgttta ctggcattct cgatcacgtc gtccaaatca
      301 tctttcgcaa aaagtgaaac tgatgcggcc aaaaaaatca tgccgtattt caaccatttc
      361 atacccaaaa ctcctcttaa aaatccccag cccttcagcc gtgccattcg cgcgctacaa
      421 gagattcatt tttattaaaa taaattttat taaacttaat aatctcaaaa ttgggactca
      481 aatcgtaatc accgggtacg atttgtgaaa aattaactcc cgacaaaaca ggaaagccca
      541 cctgattgta cgcttccgcg atcaggccgg agcatacttc attggaaccg gaaatatctc
      601 ctaaaaactc agtataatcc actttctttt taaaaaaact gaaaagcagg ttactgatat
      661 tgatgtgatc gtaaccccgc ccgacgtgtg acatggcaaa agaaactact ttgtctatgg
      721 cctcaccgct caatccgaaa gggcggcaaa ctcgaatatt gaggtgcgca agcgcctcaa
      781 ccggattgac taccgttccc gtcgaaggga gcggatcaat gatcttcccg tcacccacat
      841 aaagcgcaca atgactccat gtcgaatggg ttacggccat aataacgcgg cttatcaatt
      901 tattaccttc aaccaaaata acatcaccgg gcttcagcgc tcgcttaagg ttttccatgt
      961 tattgcgtgt acgtaagtga taactgataa gatcccgatt gagtctttcg gcaagtaccc
     1021 gtgcggcttt tttctggacg gcttcccaca cccatagcag agggtttgac attttcaaaa
     1081 tccaaacata agtaatacat cgcaaaatcc gctattgcta ttattgtact tcttttgcct
     1141 tccattttca atatctatgt agttgcataa aagttgtgga atggatatgt tgtcgggtca
     1201 ttttatccct agcccacgat aatcaaagga gcatatcatg tctcaagtac gacgctacag
     1261 tttgatgatc agtgtttttc tgttatttgg atacgcgctt acggcgcaaa gtaaacgccc
     1321 aattactgcg gacgatctat gggcatttaa gcgtctgtcg ggtttcacgg tgtcacccga
     1381 taataaatat ggttgttttg ttgtcacaga ctacaatgtg aaagaaaaca aaggtaattc
     1441 agatctgtat ttgatcgaat tggccacagg aaaaacaaaa cgccttacaa ccaatgtcgg
     1501 cagcgacaac tcgcctgcat ggcaccccaa cagccgaacg atcgctttta tttccaaacg
     1561 tgaaggcgat tacgcccaac ttcatcagat agacattgac ggtggcgaag cctccttgat
     1621 cacagagatg ccgatgggtg ccgtcgctcc taaatatttt cctgacggaa aacgcattgc
     1681 tttcgtgagt acgatattgc ccgcttatga aaatgatttt gacgggatga aaaaaaactt
     1741 aaaagaacgc aaagattcca aaatgtcggc taaagtttct gaaaatcgtt ttttccgtta
     1801 ttgggataca tggctgacag acggttatgt cccgcattta tacagttttc acatcgaaac
     1861 taaagaacgc aaagacctta caccgggact caatgaattg gtcagttccg atggctcttt
     1921 ggattacgat atcagcccgg acggtcaatg gataactttt acccgtaata gttctaatcc
     1981 tccgtacgca cagctcaaca gcgatgtttt tctttttaag gctggcgaat acgcttcggc
     2041 ggtcaatatc acggcagata atctcgatga cgacggcggt gcggtttttt ccaaggacgg
     2101 taaatcgttg tggtatagcc gttcgtttga aaaaaatcat cccgatctga gccatgtcgt
     2161 ccgctatgat ctgacgtcaa aagaacataa agtcataacg gataaaatgg atcgcagttt
     2221 cggttcctac acggagtccg ataataaaaa agattggtac tttgtagcgg aagacatggg
     2281 gcgtacgtct gtatataact ataatacgca aaaccaaaaa attgaaaaaa tattcaccga
     2341 ggcagcatgg ccaatgtgaa tgtgggtacc gacaaacttt ggttcttacg cacaagtatt
     2401 tcatcccctg ctgaaatctg ggaatataat ttcaaaacca aaaaagccgc gtcggtaacg
     2461 tcgtttaaca aatcgctctt agattctttg gattttggca aaacgga
//
LOCUS       DAOQNQ010000406         2480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_24768_length_2480_cov_5.462268, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000406 DAOQNQ010000000
VERSION     DAOQNQ010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24768_length_2480_cov_5.462268"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2480
                     /locus_tag="PK754_15600"
     CDS             <1..>2480
                     /locus_tag="PK754_15600"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HNB10978.1"
                     /translation="YKSMEWRQIGPFRGGRSVAVAGDARNPLTFYFGATGGGVWKTTD
                     GGITWLNVSDGFFKTSAVGALSVAESDPNVIYAGMGETCIRGNITPGDGIYKSLDAGK
                     TWSHTGLKETQFIGGIAIHPQNPDFVLVAAMGHIFGTNKERGIYKTTDGGKTWKQTLY
                     KDDKTGAVEVAFDPRNPRIVYAALWEAHRNAWSMTSGGSGSGLYKSTDGGETWTDISK
                     YPGLPKGVIGKIGISVSPVKDGLVWACIENDNGGVFRSDDGGQTWRRTNEERNLRQRA
                     WYYSHIYADPVNSESVYALNVQFFKSVDGGRTFKNFETKHGDHHDLWIDPRNSQRMII
                     ADDGGAAVSYNGGVSWTDLDIPTAQFYHVSVDNQFPYNIYGAQQDNSTVRISSRTTSY
                     GIDFADWSIVAGGESGYVVAHPSKPTVTFGGSYGGYLTRLDATTKQERNIAVWPDNPI
                     GAGAADQKYRFQWTYPIVFSPHDKNTLYVTAQCVFRSTDEGQSWTVISGDLTRNDQSK
                     QLSSGGPITKDNTSVEYYNTIFSLAESYKEKGVLWAGSDDGLIHISRDHGATWNNVTP
                     KDFPEGLISIIEPSYMDGGVCYVAATRYKFDDLQPYLFKTTDYGKTWKRITQGIPNGA
                     FTRVIRADPFRPGLLYAGTETGMFVSFNDGESWQSLQLNLPVVPIHDIAVQTREKDLV
                     VATHGRSFWILDDLTPLHQIADAWAKQAGLLYKPRDAYRMDGFQYSEPGVPVGTNPPN
                     GVLINYYLKEPPKEEVRLEFLNAEGNLITAYSSKKDKKGKTIEDSKEFYEKKKEKRMD
                     VVPADSGMNRFVWDMRYPDAAEVAGAIM"
BASE COUNT          663 a          556 c          672 g          589 t
ORIGIN      
        1 gtacaaatct atggagtggc gccaaatcgg tccgtttcgc ggaggccgtt ccgtagcggt
       61 tgccggtgac gcgcgcaatc cgttgacctt ttattttggc gcaaccggtg gcggtgtatg
      121 gaaaacgacg gacggcggta ttacgtggct caatgtatcg gacggatttt ttaaaaccag
      181 cgcggtcggt gcgttaagcg tagcggagtc ggatcctaat gtgatctatg ccgggatggg
      241 cgaaacgtgc attcgcggta atattacgcc gggcgacggt atttacaaat cgcttgatgc
      301 cggcaaaacc tggtcgcata ccggtttgaa agaaacgcag tttatcggcg gcattgcgat
      361 acatccgcag aatcccgact tcgttttagt cgccgcaatg ggacatattt ttggtaccaa
      421 taaagagcgc gggatttata aaacaaccga tggcggaaaa acgtggaaac aaactttata
      481 caaagacgat aaaaccggtg cggtcgaagt ggcgtttgat ccgcgcaacc cgcgcatcgt
      541 atatgcggcg ttatgggaag ctcatcgcaa tgcgtggagc atgaccagcg gaggttcggg
      601 ttcggggtta tataaatcaa ccgacggcgg cgaaacgtgg acggatattt ctaaatatcc
      661 cggcttgcct aaaggcgtga tcggaaaaat cggtatttcc gtttcacctg tcaaagacgg
      721 actcgtgtgg gcatgtatcg aaaatgataa cggcggtgtt tttagatcgg atgacggtgg
      781 tcagacatgg cggcgcacca atgaagaacg aaatttgcgc cagcgggcat ggtattattc
      841 acatatttat gccgatcctg tcaattcgga atcggtgtat gcgctcaatg tacagttttt
      901 caaatccgtg gatggcgggc gaacgtttaa aaattttgaa accaaacacg gcgatcatca
      961 cgatttgtgg attgatcctc gtaattccca acgaatgatc atcgctgatg acggcggggc
     1021 cgcggtaagc tacaacggcg gtgtatcgtg gaccgatttg gatattccga cagcgcagtt
     1081 ttatcatgta tccgtggata atcaatttcc atataacatc tacggtgctc aacaagacaa
     1141 tagtaccgtt cgtatcagca gtcgtaccac gagttacggc attgacttcg ccgattggag
     1201 tatcgtcgca ggaggagaaa gcggttatgt cgttgcccat ccctcgaagc cgacggtgac
     1261 tttcggagga agctacggag gataccttac acgattggac gcgaccacaa aacaagaacg
     1321 gaacattgcc gtatggcctg acaatccgat cggtgcgggt gccgctgatc aaaaatatcg
     1381 tttccaatgg acgtacccga ttgttttttc accgcacgat aaaaatacgc tatatgtaac
     1441 ggcgcagtgc gtatttcgat cgacggatga gggtcagagt tggacggtca tcagcggcga
     1501 ccttacgcgc aacgatcaat ccaaacaact atcgtccggc gggccgatca ccaaagacaa
     1561 caccagtgtc gaatattaca atacgatttt ttcgctggcg gaatcgtaca aagaaaaagg
     1621 agttctttgg gccgggagtg atgacggttt gatacatatc agtcgtgatc atggtgcgac
     1681 atggaacaat gtgacaccca aagattttcc cgaaggcctt atcagcataa tcgaaccgtc
     1741 gtatatggat ggcggcgtct gttatgtggc ggcgacgcgg tacaagtttg acgatctgca
     1801 accatatctt tttaaaacta cggattacgg caaaacgtgg aaacgtatta cgcagggaat
     1861 tccgaacggc gcatttacgc gtgtgattcg cgccgatccg ttccgtccgg gtttattgta
     1921 tgccggcacg gaaacgggca tgtttgtatc attcaacgac ggtgaatcct ggcaatcgtt
     1981 gcaactcaat cttcccgtgg ttccgattca cgatattgcc gtacaaacgc gggaaaaaga
     2041 tcttgtcgtg gcgacacacg ggcgttcatt ttggatctta gacgatctga ccccgctcca
     2101 tcagatcgcg gacgcgtggg ccaaacaagc gggattgctt tacaaaccgc gagacgcgta
     2161 ccgcatggac ggatttcaat acagcgaacc cggcgttcct gtagggacta atcctcccaa
     2221 cggagtattg attaattatt atttaaaaga accgccgaaa gaagaagtaa gactcgagtt
     2281 tttgaatgcc gagggtaatc ttataaccgc ttactccagc aaaaaagata aaaaaggcaa
     2341 aacgatcgag gattctaaag aattttacga gaagaaaaaa gaaaagcgaa tggatgtagt
     2401 tcctgccgat tcagggatga accgctttgt gtgggatatg cgttatccgg atgccgcgga
     2461 agtggccgga gcgatcatgt
//
LOCUS       DAOQNQ010000407         2477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_24843_length_2477_cov_4.758051, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000407 DAOQNQ010000000
VERSION     DAOQNQ010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24843_length_2477_cov_4.758051"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PK754_15605"
     CDS             complement(<1..687)
                     /locus_tag="PK754_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10979.1"
                     /translation="MNRFIFITIGVIGIGRLHAQNMEEKELFYGQTIFAWRASAHAAD
                     SLNQRMKENADEIVQQKKSIQPDKERIARSLAKGLTLSEQIKANQRRMEDYHKRIETL
                     RIQLNSEYDYIIDSLRIQQKRQNTSSIEKQLRYWTEKKLLITPPMNNLSYDPVTLLKI
                     DLYKITDPFEKEVRKEYLQAAIIEVDKYVDQIRTQRKVYESLVSLRKRTTDFIQDAYD
                     AGTWAGFSRNG"
     gene            complement(684..2048)
                     /locus_tag="PK754_15610"
     CDS             complement(684..2048)
                     /locus_tag="PK754_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNB10980.1"
                     /translation="MKIMIVEDNASMRMGLVESLRREGFEVHAFSDGESALKFFKQKP
                     LSLVITDLKMDGMSGMDVLEQVKTIQSQTAVMVVSAFGTVETAVHAMRKGAADFLTKP
                     FSPEELRFRVGRLVEKIRQEIILTRLKEENELLQGELLRSYGAMIGTSQGIKKIFNLI
                     DRVASEDSTILIEGESGTGKELVARAIHQKSNRHDKPFVKVNCGALSENLLESELFGH
                     EKGAFTGAIKRKKGRFELADGGTLFLDEIGDISQAMQVRLLRVMQEREFERVGGEDTI
                     AVNVRIIAATHRSLSKMVQEEKFREDLFYRLRVIPISLPPLRERQEDIPLLVHHFLNQ
                     YAGTRGQSSKQISDEAMETLKKHAWPGNIRELQNLIERLCVISENEIIDDAMITQHIT
                     GPSKSLMSGFENLPLEQALEAFEKGIIMAALKKSNNIKNQAAKLLGIPTSSLYYKMEK
                     FQLL"
     gene            complement(2064..>2477)
                     /locus_tag="PK754_15615"
     CDS             complement(2064..>2477)
                     /locus_tag="PK754_15615"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB10981.1"
                     /translation="LCVEVEQLLLNEIKNKKIEINYSLSVPMIFFDPQHFRQIMLNLF
                     ANAVEALEMFGKIEVESKIHDSYIHVSIRDNGKGIDEKWLSQVFEPFFTRSGHGNGLG
                     LAICKKLCTANGATIHIENRPESGCSVTMRLKQTI"
BASE COUNT          652 a          569 c          467 g          789 t
ORIGIN      
        1 accattacga gaaaaaccgg cccaggttcc cgcatcatat gcatcttgta taaaatccgt
       61 cgtacgtttt ctcagagaca ccagggattc gtatactttt cgttgcgtac gaatctgatc
      121 tacatattta tccacttcga taatagccgc ctgtaggtat tctttgcgaa cctctttttc
      181 aaaaggatcc gtaatcttgt acaaatctat ttttaataat gttaccggat catacgatag
      241 attgttcatc ggaggcgtga tgagtagctt tttttctgtc caatatcgca attgtttttc
      301 gatagaagaa gtgttttggc gtttttgttg gatacggagc gaatcaatga tatagtcgta
      361 ttcgctgttg agttgaatac gtaacgtttc gattcgttta tgatagtcct ccatccgtcg
      421 ttgattggcc ttaatttgtt ctgacaaggt caatccttta gcgagtgatc gtgctatacg
      481 ttctttatcg ggctgaatgg attttttttg ctggacaatt tcatctgcat tttctttcat
      541 gcgctgattg aggctgtctg ctgcatgtgc cgatgcacgc caagcaaaaa tagtttgccc
      601 gtaaaacaac tccttttctt ccatgttttg tgcatggaga cggcctatgc caatcacccc
      661 aatcgtaata aaaataaacc gattcataag agttggaatt tttccatttt ataataaagg
      721 ctgctcgtag gaatgcctaa tagttttgca gcctgatttt tgatattatt gctctttttc
      781 aatgcagcca taattatacc cttttcgaaa gcttccaacg cctgctccaa cggcaaattc
      841 tcaaaacccg acatgagcga tttagaagga cccgtgatat gttgagtaat catggcatca
      901 tctattattt cgttttcaga tataacacac aaacgttcaa tcagattttg caattcgcga
      961 atatttcctg gccaagcatg ctttttcaaa gtttccatcg cttcgtccga tatctgtttt
     1021 gaactttgac cgcgggttcc ggcatattga ttgagaaaat gatgcacgag cagcggtata
     1081 tcttcctgtc gttcacgaag cggcggcaac gaaatgggaa tgacccgtaa tctataaaaa
     1141 agatcttcac gaaatttttc ttcttgcacc attttagaca gcgagcgatg agtggcggct
     1201 ataatgcgta catttacggc aatcgtatct tcaccgccga cacgttcaaa ttctctttcc
     1261 tgcattactc gaagtaaacg cacctgcatg gcctgcgaaa tgtcaccgat ctcgtcaaga
     1321 aacagggttc caccgtcagc taattcgaaa cgacctttct ttcttttgat agccccggta
     1381 aaggcgcctt tttcatgccc aaaaagctca ctttcaagaa gattttctga caatgctccg
     1441 caattcactt tgacaaacgg tttatcgtga cgattacttt tttgatgtat agcgcgcgct
     1501 acaagctctt taccggttcc gctttcgcct tcgataagga tcgtactgtc ttccgatgcc
     1561 actcggtcaa tcaaattgaa tattttttta ataccttgcg atgtccctat catcgcgccg
     1621 tagctgcgca gcagttcgcc ctgtaataat tcgttttctt ccttgaggcg tgttaaaata
     1681 atttcctggc gaattttttc gacaagcctg cctacccgaa aacgtaattc ctccggtgaa
     1741 aacggttttg tgagaaaatc agcggcgcct tttcgcatgg catgtacagc cgtctcgacc
     1801 gtaccaaagg ccgaaacaac cataacggct gtttgcgatt ggatcgtttt gacctgttct
     1861 aagacgtcca tgcccgacat gccatccatt ttcaaatccg tgatgaccaa cgataacggc
     1921 ttttgtttaa aaaattttaa agcactctcc ccatcggaaa aggcatggac ttcaaaccct
     1981 tcgcggcgta aactttccac caatcccatt cgcatggaag cgttatcttc aacaatcatt
     2041 attttcatgg attcctgcat acgtcaaatc gtttgtttaa gccgcatcgt cacagagcaa
     2101 cctgattcag gacggttttc gatatggatc gttgccccgt tggcggtaca aagtttttta
     2161 cagatcgcaa gtcctaatcc gttaccatgt ccgcttctcg taaaaaacgg ttcaaaaacc
     2221 tgagatagcc atttttcatc aatcccctta ccgttatcac gaattgatac atgaatataa
     2281 gagtcgtgta ttttagattc tacttcgatt ttcccgaaca tttcaagcgc ttcaactgca
     2341 ttggcaaaca aattaagcat aatctgacga aaatgctgtg gatcaaaaaa aatcatgggt
     2401 acgcttaagc tgtaattaat ttcaattttt ttatttttta tttcgttcaa aagtaattgc
     2461 tccacttcga cacacaa
//
LOCUS       DAOQNQ010000408         2470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_24982_length_2470_cov_5.493996, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000408 DAOQNQ010000000
VERSION     DAOQNQ010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24982_length_2470_cov_5.493996"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PK754_15620"
     CDS             <1..192
                     /locus_tag="PK754_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10982.1"
                     /translation="IIDWNHRIEDLEKRKRIKFHMVEKYYAEVQQSLDEFKNLPNFVM
                     DLKFDVDLAFENLKHTIKE"
     gene            189..656
                     /locus_tag="PK754_15625"
     CDS             189..656
                     /locus_tag="PK754_15625"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HNB10983.1"
                     /translation="MKSVVSSEGQPSLSFVEESSAFSVWPHVTYLVIYFMVAFLGIAL
                     EPSLEGVGTHRQLGLPPCGFLTITGYPCPSCGLTTSVSLGMHGHWLDALRVQPFGVYL
                     FVLMSVVAILSLVGIWKKIPFSKYIQSHTGEKLQLITASVFLISWIYKILIMN"
     gene            672..1958
                     /locus_tag="PK754_15630"
     CDS             672..1958
                     /locus_tag="PK754_15630"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNB10984.1"
                     /translation="MKLHTKIMTGLGIGLLAGIITNQIIGPEAANHPDLQWFITNITD
                     PVGQIFLRLLFMVVVPLVFASLALGVANLGNLETLGRIGVRTILYFVLLTAAAAVLGL
                     LLVQIFHPGKSFDPEMQKTLMAQYGSSTSEVIGKSKDISFSVMTFVDMLLPRNMMQAV
                     VTMQMLPIIVLALLFGVMMTRMKNNHMDSVKNILEAVNEFAVGVVDLAMRFAPMAVPC
                     LVFSVSARFGVSVLKPLGFYVILVFAGYILHLFGTYSLVLRLMAGINPLRFFQAVKPV
                     MVTAFSTSSSNATLPTTIRVTEEELKISPNICGFVLPLGATTNMNGTALYEGLTVLFL
                     AEIFGVDLTLGQQAFVVVMTVLTAIGTAGIPGGSLPMIVLVLQSVNVPAEGIGIILGV
                     DRILDMGRTVLNVTGDITAAAYIAKTQGEPAQFNEA"
     gene            2027..2437
                     /locus_tag="PK754_15635"
     CDS             2027..2437
                     /locus_tag="PK754_15635"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNB10985.1"
                     /translation="MNKNIKIIAGVLLVGGTLVFLAISGFDEGKSYYKFVDEVQAMGE
                     DAYTKKLKVHGNVVDGSIRKNGSQLEFVITRNGATMPVKYIGKDPVPDTFKDGCEAVI
                     DGRYKKDGTFEGTQIQAKCASKYETDYNDLKKGS"
BASE COUNT          643 a          478 c          592 g          757 t
ORIGIN      
        1 atcattgact ggaatcatcg tatcgaagat cttgaaaaaa gaaaacgtat caaatttcac
       61 atggtcgaaa aatattacgc cgaagttcag caatctctgg acgaattcaa aaatcttccc
      121 aattttgtga tggacctcaa gtttgatgtg gatttggctt ttgaaaatct taaacacacg
      181 atcaaggaat gaaatcggtc gtatcatccg aggggcaacc gtcactgtct tttgtcgaag
      241 agtcatccgc tttttctgtg tggccgcatg tgacatacct tgtgatttat tttatggtgg
      301 cttttttagg tattgctttg gagcccagtc ttgagggtgt cggcacgcat cgccaattgg
      361 ggttgccgcc atgtggattt ctgactatca ccggttatcc ttgcccttcg tgcgggttga
      421 ctacgtccgt gagtttgggc atgcacgggc attggttgga tgcacttcgt gtacaaccat
      481 ttggcgttta tttattcgtt ttgatgtccg tggtggccat tctgtctctc gtaggtattt
      541 ggaaaaagat tcctttttca aaatacattc aatcccatac cggggaaaaa ctgcaattaa
      601 tcacggcttc tgtttttttg ataagctgga tttataaaat tttgattatg aactaataga
      661 aagacttgaa gatgaagctt cacaccaaaa tcatgaccgg acttggtatc gggttacttg
      721 caggaattat taccaatcag atcatcggac ctgaagccgc caatcatccc gacttgcaat
      781 ggtttatcac caatatcacc gatccggtcg gtcagatatt tttacgtctt ctttttatgg
      841 tggtggttcc gttggtattt gcatcactgg ctttgggagt agccaatctc ggtaatttgg
      901 aaacattggg gcgaatcggc gtacgaacca ttttgtattt tgttttattg acagccgccg
      961 cggcggtgct gggtttactt ctggttcaga tttttcaccc gggtaaaagt tttgatccag
     1021 agatgcaaaa aacattgatg gcgcaatacg gttcatcgac cagtgaggtt atcggcaaaa
     1081 gcaaagacat tagtttttcg gtgatgacgt ttgtggatat gctattgccg cgtaatatga
     1141 tgcaagccgt ggtgacgatg caaatgctgc cgattatcgt attggcgctg ctttttggtg
     1201 tgatgatgac gcgaatgaaa aacaatcaca tggattctgt aaaaaatatt ctcgaggctg
     1261 tcaacgaatt tgccgtcggt gtggttgact tagccatgcg atttgctccg atggccgtgc
     1321 catgccttgt gtttagcgtt tcagcacgat tcggtgtgag tgtcctcaaa ccgctgggtt
     1381 tttatgttat actcgtattt gccggataca tcctacattt atttggcact tattcgttag
     1441 tgcttcgttt gatggcaggg atcaatcctt tgcggttttt tcaagcggtt aagcctgtga
     1501 tggtcacggc attttcgacc agttcaagca atgcgacatt acctaccacc attcgcgtaa
     1561 cggaagaaga acttaaaatt tcacccaata tttgtggatt tgtattacct ctgggtgcaa
     1621 ccactaatat gaacggcacc gcgttgtatg aagggttgac ggttttgttt ttagctgaaa
     1681 tttttggcgt ggatctgacg ctcggacaac aagccttcgt tgttgtgatg acggttctta
     1741 ccgcgatagg caccgcaggc attcccggtg gttctttgcc gatgattgtt ttagttttac
     1801 aatcggtcaa tgtcccggcc gaaggaatcg gtataattct cggagttgat cgtatattgg
     1861 atatgggacg gacggtgctt aatgtaaccg gcgacattac ggcggcagcg tatattgcca
     1921 aaacacaagg cgaaccggcg caatttaatg aagcttaagt tgcttttttg agtaagatga
     1981 agtattatac tgatgtaacg aatacgtaca cagaaaggtt ttttttatga ataaaaatat
     2041 caaaatcatc gccggtgttt tattagtcgg tgggacgttg gtttttttgg cgatctcggg
     2101 ctttgatgaa ggaaaatcgt attataaatt tgtggacgaa gtccaagcaa tgggcgaaga
     2161 tgcctatacc aaaaaactga aagttcacgg taatgtcgtg gatggcagta ttcgcaaaaa
     2221 cggttcacaa ctcgaattcg tcattactcg caacggtgca acgatgccgg tcaaatatat
     2281 cggaaaagat cctgttccgg atactttcaa ggacggttgc gaagccgtga ttgacggacg
     2341 ctacaagaaa gacggcacgt ttgaaggaac acagatacaa gctaagtgcg catcaaaata
     2401 cgaaacggat tacaacgatc tcaaaaaggg aagttaattc gtaaaatttt ttttcaacaa
     2461 aagatcattc
//
LOCUS       DAOQNQ010000409         2468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_25023_length_2468_cov_5.069208, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000409 DAOQNQ010000000
VERSION     DAOQNQ010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25023_length_2468_cov_5.069208"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..490
                     /gene="rfaE2"
                     /locus_tag="PK754_15640"
                     /pseudo
     CDS             1..490
                     /gene="rfaE2"
                     /locus_tag="PK754_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469050.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
     gene            666..1763
                     /locus_tag="PK754_15645"
     CDS             666..1763
                     /locus_tag="PK754_15645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNB10986.1"
                     /translation="MKYSEIKFDCRFFRGDIPCKPNKLHGKICNTCDQYQPISRRILI
                     IKLGAIGDVIRTTPLVVRYHKMFPNCHVSWITHTPEVVPSSIVDKVYKFDFKSVYALS
                     HQHFDIAINLDKDIEACALLNDVHAKEKFGFGLKDGHAAAENPLAEHKFITGAFDSIS
                     RANTKNYLEEIFEICGLTFEREPYILDVDNKYLSVADPVLRAAQGKKIIGLNTGCGDR
                     WLTRLWPEAYWVELIQILQMNGFFPLLLGGPAEDAMNKNYAKMTGAYYPGTFTLQEFI
                     ALSSRCDVVLTAVSMMMHIAIATRVPLVLFNNIFNPHEFELYDNGIMVQPATGCDCYF
                     GNTCSRTHHCMLDIPVDSVYQAIVRLKDRKA"
     gene            1760..>2468
                     /locus_tag="PK754_15650"
     CDS             1760..>2468
                     /locus_tag="PK754_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNB10987.1"
                     /translation="MKIAYLSTFYPFRGGIAQFNASLYRTFEKQHTVRAFTFTRQYPD
                     LLFPGKSQYVTPDDHADPIPSRALLDTVNPLSYRRTALELAAFAPDMLVMKFWMPFFA
                     PSLGYVAGYLRRRGTKVITVLDNVIPHEKRPGDMALIRYFLRRNSGFVAMSKTVEQDL
                     LALMPDARYTLKAHPLYDHYGPAIGRDEACAKLGIPKNKKTILFFGFIRDYKGLDLLI
                     RSVARLSESHHLIIAGEV"
BASE COUNT          630 a          567 c          597 g          674 t
ORIGIN      
        1 gtccgcaagc ggttcacgaa aaatcctgac ggccggtgaa gcgagtgtac tcatcgccgg
       61 tatacgcgga aaaaagaaaa tcgtatttac caacggttgt tttgatattc tccatgtcgg
      121 tcatatcacg ttgctcaatc aggcacgggc actcggtgat gcgctggtcg taggacttaa
      181 tagcgatgct tccgtgcggc gacttaaggg cgaaaaacgc ccgatcgttt cggagcagga
      241 acgcgcccat attcttgcgg cgctggcatc ggtagattac gtcgtgttgt ttgatgaaga
      301 tacgccgttg gaactgatac gacagattca gccggatatt ttagtcaaag gttcggacta
      361 caccttggat cgcgttgtcg gacgtgcgga agtggagtcg tatggcggac gggtcgcgct
      421 tgttgatctg gtcgtcgggt ttagtacgac caatattgtc gaatctattt tgcaggctta
      481 cggcgtaaaa caagaaaata aaacaaccga cgataaaaat ttggcccatg ctgaaaatac
      541 catgcagcgc atcacatcgt tgcaggatcg gtatgaagaa aaaattgatc gcattgaaaa
      601 taaaaacaaa tccgacgcgc taaacgcatt gcacccaaaa aagaaataat cagatcttct
      661 aatctatgaa gtactccgaa atcaaatttg attgccgctt tttccgcggt gatattccgt
      721 gtaaacctaa taaattgcac ggcaaaattt gcaatacctg cgatcagtat caaccgattt
      781 cgcgccgcat acttatcatc aagctgggcg ctatcggtga tgtgattcgt acgacgccgc
      841 ttgtggtgcg ttatcataaa atgttcccca attgccatgt atcttggatc acgcacacac
      901 ccgaggtcgt accctcttcg atcgtggaca aagtttacaa atttgatttc aaatccgtgt
      961 atgcgttaag tcatcaacac tttgacatcg caatcaactt agataaagat atcgaagcct
     1021 gtgctttgct caacgatgtg catgccaaag aaaaattcgg ctttggcctg aaagacgggc
     1081 atgcggcagc agaaaatccg ctggcagaac ataagtttat tacgggcgct tttgacagca
     1141 tctcgcgcgc caatactaaa aattacctcg aagagatttt tgaaatatgc gggcttacgt
     1201 ttgaacgtga gccctatatt ttggatgtgg ataacaaata tctttctgta gccgatccgg
     1261 ttttacgtgc cgcgcagggc aaaaaaatca tcggactcaa taccggttgt ggagaccgat
     1321 ggctcacgcg attgtggccg gaggcgtatt gggtcgaatt gatccagatt cttcagatga
     1381 atggtttttt tcctctgctt ctgggcgggc ctgcagaaga tgcgatgaac aaaaactatg
     1441 ctaaaatgac cggtgcgtat tatccgggaa catttacatt gcaggaattt atcgcgttgt
     1501 cttctcgctg tgatgtagtg ctgacagcgg tttccatgat gatgcacatt gcgatcgcaa
     1561 cgcgggtacc gctggtactt tttaataata tttttaatcc gcatgaattc gaattgtacg
     1621 ataacggtat catggttcag ccggctaccg ggtgcgattg ttatttcggc aatacctgtt
     1681 cccgcacgca tcactgcatg cttgatattc ctgtagattc ggtatatcag gcgattgttc
     1741 gactgaaaga ccgcaaagca tgaaaattgc ttatctgtcc acgttttatc ccttccgcgg
     1801 cgggattgct cagtttaatg catcactgta ccgcacgttt gaaaaacaac acactgttcg
     1861 ggcttttacc tttacgcgtc aatatcccga tcttcttttt cccggcaaaa gccaatacgt
     1921 aactcccgat gatcacgccg atccgatacc gtcccgtgcg ctattggata ccgtcaatcc
     1981 gctctcgtat cgtcgcacgg cattagaatt agccgcgttt gctccggata tgctggtgat
     2041 gaagttttgg atgccatttt ttgcaccatc gttgggttat gtggcgggtt atcttcgccg
     2101 acgcggcacc aaagtgatca ctgtgcttga taatgttatc ccgcacgaaa agcgaccggg
     2161 ggacatggcg ttgattcgtt attttctccg tcgcaatagc ggcttcgtcg cgatgagcaa
     2221 aacggtcgaa caggatttat tggcattgat gcctgacgca cgatacacgc tcaaagcgca
     2281 cccgctgtac gatcattacg gtccggctat cggccgtgat gaagcgtgtg cgaagctggg
     2341 tattccgaaa aacaaaaaaa ctattctttt ttttggtttt atccgcgact acaaaggact
     2401 tgatcttttg atccgttccg ttgcgcgatt gagtgaatcg catcatctga tcatagccgg
     2461 cgaagtgt
//
LOCUS       DAOQNQ010000410         2466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_25062_length_2466_cov_4.985898, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000410 DAOQNQ010000000
VERSION     DAOQNQ010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25062_length_2466_cov_4.985898"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1829)
                     /gene="dnaK"
                     /locus_tag="PK754_15655"
     CDS             complement(<1..1829)
                     /gene="dnaK"
                     /locus_tag="PK754_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNB10988.1"
                     /translation="MAKGKVIGIDLGTTNSCVSIMEGGQPVVIANSEGGRTTPSVVGF
                     TKNGERLIGNPAKRQSITNPKNTIFSIKRFMGRRYDEVTSERGRVPYEVIAGDNNTAR
                     VKVADNMYSPPEISAMVLQKMKQTAEDYLGQTVTDAVITVPAYFNDAQRQATKDAGKI
                     AGLNVLRILNEPTAAALAYGLDKKKNEKIAVYDLGGGTFDISILEIGDGVFEVLATNG
                     DTHLGGDDFDDLIMNYLADEFKKNEGIDLRKDPMALQRLKDAAEKAKIELSSSQQTQI
                     NLPYITAVDSVPKHLDITLTRSKFEQICDTLIQRTVKPVEQAMKDAGLQYAQIDEVIL
                     VGGSTRIPKIQQLVQDLFKREPHKGVNPDEVVAVGAAIQGAVLAGEVKDVLLLDVTPL
                     SLGIETLGGVFTAMIPRNTTIPTKKSEIFSTAADSQTAVTIRVGQGERQMFADNRELG
                     RFELMGIPPAPRGVPQIEVTFDIDANGILHVTAKDKATNKEQQIRIESSSGLNKDEID
                     RMVNQAQEHAEEDKKRREKIDTKNQAESLAYQTEKNIKEFGDKVDADSKSRLEGEMQK
                     LKDAIATDNTDTMKSAMESLSAIWNEVSSKMYQAGAGAQGATGA"
     gene            complement(2128..>2466)
                     /locus_tag="PK754_15660"
     CDS             complement(2128..>2466)
                     /locus_tag="PK754_15660"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNB10989.1"
                     /translation="LGRTYLMAEMQMLWKLPKRKWFPVLGGVEILFLKSIDPFQKFDI
                     VTHVLTWDAKWIYVEQRFERNGVVLAKAHLRALFLGPQGRVATDAIIALAELGSVEAP
                     LHPVLQKWKD"
BASE COUNT          612 a          601 c          512 g          741 t
ORIGIN      
        1 gcaccggtcg cgccctgagc accggcacct gcctgataca ttttagaaga cacttcattc
       61 caaatcgcac tgagcgactc catggccgat ttcatagtgt cggtgttatc cgtagcgata
      121 gcgtctttca gtttttgcat ttcaccctct aagcgtgatt tgctgtccgc atcgacttta
      181 tctccgaatt ctttgatatt tttttccgtc tgatacgcca agctttcagc ctgatttttg
      241 gtatcgattt tttcacgacg ttttttgtct tcttcggcat gttcctgcgc ttgattgacc
      301 atacgatcaa tctcgtcttt gttaagaccg gatgacgatt cgatacgaat ttgctgctct
      361 ttattggtcg ctttatcttt ggccgtcacg tgcaaaatac cattggcatc aatatcaaat
      421 gtcacttcga tctgcggtac accgcgcggc gcaggcggaa tgcccatcag ttcaaaccga
      481 cccagttcgc ggttatcggc aaacatttgg cgttcgcctt ggccgacgcg aatcgtcacc
      541 gccgtctgac tgtcggccgc tgttgaaaaa atttcgctct tcttcgtcgg gattgtcgtg
      601 ttgcgcggaa tcatcgcggt gaataccccg ccgagcgttt cgataccgag tgaaagcggt
      661 gtcacatcaa gcaacaatac gtctttgact tcgcccgcaa ggacggcacc ctgaatagcc
      721 gcaccgacgg cgaccacttc atccggatta acacccttgt gcggttcgcg tttgaaaagg
      781 tcttgcacaa gctgctggat tttgggaata cgcgtcgaac cgccaacgag aataacctca
      841 tcaatctgcg catattgcaa accggcgtct ttcatcgcct gctcgaccgg tttgacggta
      901 cgttggatca acgtatcgca aatttgttca aatttggaac gtgtcaatgt gatgtccaaa
      961 tgcttcggca cgctgtctac agccgtgatg tacggcagat tgatctgcgt ctgctgagag
     1021 ctcgacaact caattttagc tttttcagct gcatctttga ggcgttgcaa agccatcgga
     1081 tctttgcgaa gatctatgcc ttcatttttc ttaaactcgt ccgcaagata attcatgatc
     1141 agatcatcaa aatcatcgcc gccaagatgg gtgtcaccgt tggtcgccaa tacttcgaaa
     1201 acaccgtcgc cgatttcgag aatcgaaata tcaaatgtac cgccaccaag atcatagacg
     1261 gctatttttt cgtttttctt cttatccaga ccgtaggcca acgccgcggc tgtaggttca
     1321 ttgagaatac gcaatacatt gaggcctgcg atttttccgg catcttttgt cgcctgacgt
     1381 tgcgcgtcat taaaataagc cggcacggta ataacggcat ccgttaccgt ttgaccgagg
     1441 tagtcctccg cagtttgttt cattttttga agcaccatag ccgaaatttc cggcggtgaa
     1501 tacatattat ccgctacttt gacacgtgcc gtattattat cgccggctat cacttcgtaa
     1561 ggtacacgtc cacgctcact ggttacttcg tcataacggc gacccataaa acgtttgatt
     1621 gaaaaaatcg tatttttggg attggttatg gattgacgtt tggccggatt accgatcaaa
     1681 cgctcaccgt ttttggtaaa acccacaacc gacggtgttg tacgaccacc ttcagaattt
     1741 gcaatgacaa ccggttgacc gccttccata atcgaaacac atgaattcgt tgttccgaga
     1801 tcgattccga ttactttacc ctttgccata ccattactcc ttttattttc aaatgtttat
     1861 tttaagtaaa ctttttttcc tgacattaat tcagcaaagc ttgtgccgct aattttgata
     1921 ctgattttaa ataacttacg attaaatata atgaaaaatt gtcatgaaaa tttcattcaa
     1981 tgacaaaacg gcatcgtgtc gcttgcttta ttgacaagac gtcatgccgc ttaatttgaa
     2041 gcatttagat tggaatgagg aagctttagt acgacgatag taatggatac attatattac
     2101 aattacaatt tcgataaatt tagttattta atctttccat ttttgtaaaa ctggatgcaa
     2161 aggcgcttcg acagaaccta attcggctaa agcaattatg gcatccgtag caacacggcc
     2221 ttgcgggcct agaaataatg cgcgcagatg ggctttggcc aaaactaccc cgtttctttc
     2281 gaaacgctgt tccacataaa tccatttggc atcccaagtg agaacgtgtg tcacgatatc
     2341 aaatttttga aaaggatcta ttgatttcag aaacaggatt tccactccgc ccaatacggg
     2401 aaaccatttt cttttgggta atttccagag catttgcatt tccgccatca gatatgtacg
     2461 tccaag
//
LOCUS       DAOQNQ010000411         2457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_25259_length_2457_cov_6.024563, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000411 DAOQNQ010000000
VERSION     DAOQNQ010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25259_length_2457_cov_6.024563"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PK754_15665"
     CDS             <1..331
                     /locus_tag="PK754_15665"
                     /inference="COORDINATES: protein motif:HMM:NF018917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HNB10990.1"
                     /translation="LFLITPLVLLPLMLLHLIPGFGGVLYAILMFGYVSFATAFSFLD
                     YVLENRRFGFREKLRFVMTNRAFCFGFGGVSALLLMIPFINVLFIPVLAVSATLMYIR
                     EPMQQKR"
     gene            340..1854
                     /gene="hutH"
                     /locus_tag="PK754_15670"
     CDS             340..1854
                     /gene="hutH"
                     /locus_tag="PK754_15670"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HNB10991.1"
                     /translation="MTEFAIHGDNLSIAVISEILKNKPRVVLTESAKQTINRGRRTIE
                     DIVSSGRVVYGVNTGFGKFSDVRISDDQLEILQENLVRSHACGVGEPFPADIAKVMLL
                     LKANGLAKGYSGVRLEIVETLLAMFNADVLPVIPQQGSVGSSGDLAPLAHLTLVLMGE
                     GEAWFQGQRLPGGEAMKRAGIKPVRLRSKEGLAVLNGTQTMTATAVVNLIRAKNCMEA
                     AEICGAMSMEVLLCTPTAFDPRIQDVRNQTGQIESARRLRDLVAGSPLIASHADCKKV
                     QDAYSVRCMPQVHGATRDAIAYVQQIVEREINAATDNPLIFSEDGDVLSGGNFHGQPV
                     ALVMDFLAIAVSEIGNISERRTAWMMDGHLSDGLPPFLAKDGGLNSGYMIAQYAAAAL
                     VSENKSLSHPASVDSVPTSANKEDHVSMGTIGARKAYRIIENVENILAIEWLCAAQAG
                     DFRKPIAFAGKTQKAYDLLRKYVPTLDKDRNTSVDIQTANAQLQSGAMVNAVWG"
     gene            1899..>2457
                     /locus_tag="PK754_15675"
     CDS             1899..>2457
                     /locus_tag="PK754_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNB10992.1"
                     /translation="MKLYEALERQLKKEPNFVTDNGELKKWVVLNKAQNFDEELITLL
                     LKDETLKQKFFRTFSLSKGEVVTVFNQNLFAQFLEQKNYLNDSYTQYKNKVGLTIDGK
                     YLKQRNEVALVWPFKDCILEGGQSREEDKREEIFFNEILAQDEITQLLEPKVLTNAKR
                     IDKDGEKPLDQFNRNENHTITDNLII"
BASE COUNT          666 a          542 c          650 g          599 t
ORIGIN      
        1 tttgtttctg ataacgcctt tggttttact gccgctcatg ttgctccatt tgatccccgg
       61 cttcggaggc gtgctctatg cgatactgat gttcgggtat gtcagtttcg cgacagcatt
      121 cagttttttg gattatgtat tggaaaaccg ccgatttggt tttcgcgaaa aactgcgttt
      181 tgtaatgacc aatcgcgcct tctgttttgg ttttggcggt gtttccgctc tgcttttgat
      241 gattcctttt atcaatgtac tttttatacc tgtgttggcc gttagcgcca cgctcatgta
      301 tatacgtgaa cctatgcaac aaaaacggtg aatggagcta tgacagaatt tgccatccat
      361 ggagacaatc tgagtattgc ggtgatttca gaaattctga aaaacaaacc ccgcgtggta
      421 ttgaccgaat ccgctaaaca aactattaac cggggacgcc ggacgatcga agacattgtc
      481 agctcggggc gtgtggtgta cggtgtgaat acgggattcg gaaaattcag cgacgtacgc
      541 atttccgacg atcagcttga aattttacag gaaaacctgg tgcgcagcca tgcctgcggc
      601 gtcggtgaac cttttccggc agacatcgca aaagtgatgt tacttctcaa agccaacgga
      661 ttagccaaag ggtattccgg cgtgcgattg gaaatcgtcg aaacattatt agccatgttt
      721 aatgcggacg tcctaccggt gataccacaa caaggttccg tcggttcgag cggagatctg
      781 gcgccgctgg cgcatttgac tttggtgttg atgggcgaag gcgaagcttg gtttcagggg
      841 caacgtctgc ccggcggcga agctatgaag cgcgcaggaa taaagccggt acggcttcgc
      901 tccaaagaag gcctggctgt gctcaatggt acgcaaacga tgacggctac ggcggtcgtc
      961 aatctcatac gtgcaaaaaa ttgtatggaa gcggctgaaa tctgcggcgc catgtccatg
     1021 gaagttttgc tgtgtacgcc gacggcgttt gatccgcgta tccaggatgt gcgaaatcaa
     1081 accggtcaga tcgagtccgc gcgtcgtctg cgagatctgg tcgcgggcag tcctctgatc
     1141 gcgtcccatg ccgattgtaa aaaagtgcaa gatgcgtaca gcgtgcggtg catgccgcag
     1201 gttcatggcg cgacgcggga tgcgatcgcg tatgtacaac agatcgtcga acgggagatc
     1261 aatgcggcga cagataatcc gctgattttt tcggaagacg gcgatgtgtt atcgggtggt
     1321 aattttcatg gccagccggt cgcgctcgta atggattttc tggcgatagc cgtgagtgaa
     1381 atcggtaata tttccgaacg tcgcacggca tggatgatgg acgggcattt gagcgacggt
     1441 ttgccgccgt ttttggcaaa agatggcggg ctcaactccg gctatatgat cgcacaatac
     1501 gcggccgcag cgctcgtatc ggagaataaa tcgttgagcc atccggccag tgtagactcc
     1561 gtaccgactt cggccaataa agaagatcac gtcagcatgg gtactatcgg cgcacgcaaa
     1621 gcctaccgga ttatcgaaaa cgtcgaaaac attttagcca tcgaatggct gtgcgcggcc
     1681 caggcgggcg attttcgcaa gccgatcgca tttgccggca aaacgcaaaa agcatacgac
     1741 ttactccgga aatatgtgcc gactttggat aaagaccgca ataccagtgt ggatattcaa
     1801 acggccaatg cgcagttaca gtcgggtgct atggtaaatg cggtgtgggg gtaggggttt
     1861 aatcagacga taaagattgt ttaacatagg atagctgtat gaaactctat gaagcattag
     1921 aacgtcaact gaaaaaagag cccaacttcg tgacggacaa cggagagctg aaaaaatggg
     1981 tggtgctgaa taaggctcaa aactttgatg aagaacttat aacgctttta ttaaaagatg
     2041 aaacgttgaa acaaaaattt ttccgaactt ttagcttgtc gaaaggtgag gtcgttacag
     2101 ttttcaacca aaacttgttt gcgcagtttt tggagcagaa gaactacctg aacgacagct
     2161 acacccaata caaaaacaag gtcggactga ccattgacgg caaatacctg aaacaacgca
     2221 acgaagtggc tttggtttgg ccattcaaag actgcatttt ggaaggcgga caaagccgtg
     2281 aagaagataa aagagaagaa attttcttca acgagatttt agcacaagac gaaattaccc
     2341 aactcttaga accaaaagta ttgaccaatg ctaaacgcat tgacaaagac ggagaaaagc
     2401 cgcttgacca atttaaccga aatgaaaacc acacgataac tgacaacctg attataa
//
LOCUS       DAOQNQ010000412         2443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_25548_length_2443_cov_4.432998, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000412 DAOQNQ010000000
VERSION     DAOQNQ010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25548_length_2443_cov_4.432998"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..135)
                     /locus_tag="PK754_15680"
     CDS             complement(<1..135)
                     /locus_tag="PK754_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNB10993.1"
                     /translation="MSGILYIVATPIGNLEDITLRAIRVLKEADTIACEDTRQSKILF
                     D"
     gene            complement(132..542)
                     /gene="rnpA"
                     /locus_tag="PK754_15685"
     CDS             complement(132..542)
                     /gene="rnpA"
                     /locus_tag="PK754_15685"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNB10994.1"
                     /translation="MISSTSFPPVTVMNDPIDEAFVNRRLPRRMIIRHKDAFERMFQN
                     GRRRNGRFVQLIIVPQDSAKASDLMVAFVCGKRIGHAVQRNLYKRRLREIFRKNKFHF
                     RGWHILLIAFPAIRHAKFEELEADVLRAAEGFRL"
     gene            complement(579..1670)
                     /locus_tag="PK754_15690"
     CDS             complement(579..1670)
                     /locus_tag="PK754_15690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB10995.1"
                     /translation="MPEQTGKTIIAVDDSELNLMMMQSFLEPMGHNVIVATNGKDALE
                     LIRTENPDLILLDVMMPEMDGFEVCRRIKSNDRTRHIPVIMITALDKVEDNVKGIEAG
                     AEDFLTKPFDAVILAARMKALLQSKALFDEIAKLERLKEDMTSMIVHDLRTPMSSIKM
                     SLEFLRDNIREEKLLDFISIASADIEDSLLLINNLLDIGKLEANKMEIRKEDTSLLDM
                     LRDIAGRAKPLLMRGGLQLSVQSDRDEIFCEVDKILVGRVVTNLLANAIKFAESKSMI
                     LLDATQLDNGDVVFGVVNQGTNIPESYKKTIFEKFGQAKDQHNKAGTGLGLTFCKHVA
                     EAHRGKIWVESPPKNFPSGAAFYVQLPLR"
     gene            complement(1685..>2443)
                     /locus_tag="PK754_15695"
     CDS             complement(1685..>2443)
                     /locus_tag="PK754_15695"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNB10996.1"
                     /translation="LFVYGGKADTDWIRFETEDPDIAQIHWSQPERDVFCVTVSLHSP
                     LSWGYEVRYESSNLVWRIYHKPKQTSLKGLRICIDPGHSKDPGSVGPRRTTEKQANVE
                     IAQWLKELLIADGAEVLMTHEDTTKGLTLYERVQFADANNCDLFVSIHHNALPDGVNP
                     LRSNFGHSVIYYHPHSRLLGEKILESLVNKVKLPNYGVFQGNIAVVRNSRMPAVLVEN
                     AFIMLPDQEKKIRDPKFQQTCAEAIRDGLRQFMK"
BASE COUNT          621 a          626 c          523 g          673 t
ORIGIN      
        1 atcgaataaa atttttgatt gacgcgtatc ttcgcaggcg atcgtatcgg cttctttgag
       61 cacacggatc gctcgcagcg tgatatcttc cagattgccg atcggtgtag cgacgatgta
      121 taatattccg ctcataatct aaatccttcc gcggcacgca aaacatccgc ctccaactct
      181 tcaaacttcg catggcgaat cgccggaaaa gcaattaata aaatatgcca tccgcgaaaa
      241 tgaaatttgt ttttcctgaa aatttcacgc aaacggcgtt tatacagatt gcgttgcacg
      301 gcatggccga tacgtttgcc gcacacaaaa gcgaccatca agtcggatgc cttcgcgctg
      361 tcttgcggta cgatgatcag ttgcacaaat cgtccgtttc tccttcggcc gttctgaaac
      421 atacgctcaa acgcatcctt atggcgaatg atcatacgcc gcggcaatcg gcggttaaca
      481 aaagcttcgt ctatcggatc attcataacg gttacaggcg gaaatgatgt agaggaaatc
      541 acaacgcaaa ccttgttttt tcgtcacaag gaatctattc atcgtaatgg caactggaca
      601 taaaacgcgg cgccgctcgg aaaattcttc ggcggacttt caacccatat ttttccgcga
      661 tgcgcttcgg caacatgttt acaaaatgtc agacctaagc cggtaccggc tttgttatgt
      721 tgatctttag cctgaccaaa tttttcgaaa atggtttttt tgtacgactc cggaatattc
      781 gtgccttgat tgaccacgcc aaaaacaacg tcaccgttgt caagctgtgt cgcgtccagc
      841 aaaatcatac tcttggattc ggcaaatttg atcgcattag ccaataaatt ggtaaccacc
      901 ctaccgacta atattttgtc cacttcacaa aatatctcat cccgatcgga ttgtacactc
      961 agttgcaatc ctccgcgcat taaaagcggc ttggcccgtc cggcgatgtc acgcagcata
     1021 tctaaaagag aagtgtcttc tttgcgaatc tccatcttgt tggcttccaa tttgccgata
     1081 tccagcaaat tattgatcag cagcagcgaa tcttcgatat cggcggacgc gatactgata
     1141 aaatcgagta atttctcttc gcgaatatta tcgcgcagaa actcaagtga cattttgatg
     1201 gaactcatcg gtgtacgcag atcatgcacg atcatgcttg tcatgtcttc tttgagccgt
     1261 tccaatttgg cgatttcgtc aaataacgct ttcgattgca ataatgcttt catacgcgcg
     1321 gcgagaatca ccgcatcaaa cggtttggtc aaaaaatctt ccgcgccggc ttcgatacct
     1381 ttgacgttat cttcgacttt atccaaagcc gtaatcatga ttacgggaat gtggcgcgtc
     1441 ctgtcgttac ttttgatacg acggcaaact tcgaagccgt ccatttccgg catcatcaca
     1501 tccaacaaaa tcaggtccgg gttttcagtg cgaatcaatt ccaatgcatc tttaccgttg
     1561 gtggcgacga tgacattgtg tcccatcggc tccagaaaac tctgcatcat catgagattg
     1621 agttcgctgt catccaccgc gataatagtt ttccccgtct gttcaggcat aaagcaccaa
     1681 aggtttattt cataaactgc cgcaaaccgt cgcgaatggc ttccgcgcac gtttgctgaa
     1741 acttgggatc gcggattttt ttctcctgat ccggtaacat gataaatgcg ttttccacga
     1801 gcaccgccgg catgcggctg ttgcgcacta cggcgatatt gccctgaaag actccatagt
     1861 ttggtaattt aactttattt acgagagatt cgagtatttt ttctcccaag agccggctgt
     1921 ggggatgata ataaatcacg ctgtgaccaa agtttgaacg gagcggatta acgccgtccg
     1981 gcaaagcgtt gtgatgaatg ctgacgaata aatcgcaatt attcgcatcg gcaaactgca
     2041 cgcgctcgta caatgtcaaa ccctttgtcg tatcttcgtg cgtcatcagt acttccgctc
     2101 cgtcggcgat gagcagttct ttcaaccatt gcgcaatttc gacattagcc tgtttttccg
     2161 tcgtacggcg cggtccgaca gaacccggat ctttgctatg cccgggatca atacatatgc
     2221 gcaaaccttt gagcgatgtt tgtttaggtt tgtggtaaat acgccatacc aaattgctgc
     2281 tttcgtaacg cacttcgtac ccccagctca aaggagaatg taagctgacc gtcacacaaa
     2341 acacgtcgcg ttccggctgc gaccaatgaa tctgcgcgat atccggatcc tccgtttcaa
     2401 atcgtatcca gtcggtatcc gctttgccgc cgtacacgaa cag
//
LOCUS       DAOQNQ010000413         2442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_25564_length_2442_cov_5.478425, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000413 DAOQNQ010000000
VERSION     DAOQNQ010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25564_length_2442_cov_5.478425"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..486
                     /locus_tag="PK754_15700"
     CDS             19..486
                     /locus_tag="PK754_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10997.1"
                     /translation="MNPKYKIELLAEGPKAHIYTIRNLDSEISEFERFADEEDVRYDP
                     GFQEILARIDDMANRLGCIDSFFRNESTRDNAICALFAPDGKLRLYCIKMRFTLLIIG
                     GGGIKNTRTYQEDPHLDSCVTLLEDFWKRFALYEDNGEIEISSDGKITGKDKI"
     gene            489..914
                     /locus_tag="PK754_15705"
     CDS             489..914
                     /locus_tag="PK754_15705"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNB10998.1"
                     /translation="MSSVKNKFQELLKQVPDEVKLYVQMQGDIAIRVGEIIKEKQWTQ
                     KSLAEKMGWNESQVTRLLAGHENLTLKTIAKLSVVLEENLIQTQPRVVYIPLFPLDTP
                     ANAALNLGALRAASSVGNFFPLSTNKVTSFIKTEVKAVA"
     gene            917..1360
                     /locus_tag="PK754_15710"
     CDS             917..1360
                     /locus_tag="PK754_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB10999.1"
                     /translation="MSDPIEQGLGPSFKLTGFVIDSFSLENEARQVENVDADMLLYHH
                     QGNAEIIPPKKLIRIKLSVTAEYEGKTLGKIVTQTEFEIINFEVFINNEILNIPGELM
                     SMLVGLAYSTTRGALIAKSEGTVLAKFPMPVVSPSRLIGIAPMPS"
     gene            1554..1874
                     /locus_tag="PK754_15715"
     CDS             1554..1874
                     /locus_tag="PK754_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNB11000.1"
                     /translation="MKLGIIIETKEPEKAWNAFRFAVTSRKTGHEVKVFLMGEAVECE
                     GLTHEKYNVDEQLKKFIGEGGTILACGTCLKSRQLDGTESCPISTMTDCVQMVEWADK
                     VVTF"
     gene            complement(1887..>2442)
                     /locus_tag="PK754_15720"
     CDS             complement(1887..>2442)
                     /locus_tag="PK754_15720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HNB11001.1"
                     /translation="DDDRLASAICIFPRNDADSVFGGTGSAVEKFALYRRAAAGRFVA
                     GKYITNGVIGYRVKIKKSPIHGRGVFADEKIPARRKIGEMTGAIITVREARRRAKQRG
                     AIALVEFSDGWAVDGFDHGNAFARINHSCAPNAWMRVYGHHVEFYARRVIRVGEEITC
                     DYVESHHDGKLRCRCGAKRCRGSI"
BASE COUNT          734 a          558 c          536 g          614 t
ORIGIN      
        1 aaaaaacttt acttataagt gaatccaaag tataaaatag aattattagc cgaaggccca
       61 aaagcacata tctacacgat tcgcaacttg gactctgaaa tatcagagtt tgaaagattt
      121 gctgatgaag aagatgtacg ttatgatccc ggttttcagg aaatattagc acgcattgat
      181 gatatggcca atcgtcttgg gtgcattgac tctttttttc gcaatgaaag cacacgtgac
      241 aatgcgattt gtgcactttt tgcacccgac ggaaaattac gattatattg tatcaaaatg
      301 cgttttactc ttctgatcat aggaggcggt ggcataaaaa atacgcgaac ctaccaagag
      361 gatcctcatt tggattcttg cgttacatta cttgaagact tttggaagcg ttttgcacta
      421 tacgaggaca acggagagat agaaattagc tccgacggca aaatcacagg aaaggataag
      481 atatagatat gagttctgta aaaaataaat ttcaggagct tttgaaacag gttcccgatg
      541 aagtgaagct ctatgtgcaa atgcagggag atatagcaat ccgcgtaggc gaaataatca
      601 aagaaaaaca atggactcaa aagagtctgg ccgaaaaaat gggatggaac gagtcccaag
      661 taacgcgcct tttggccgga catgaaaatt tgacgctaaa gacgattgcc aaactatcgg
      721 ttgttttgga agagaatttg atacaaacgc aaccaagggt cgtgtacatt ccccttttcc
      781 cgttggatac accggccaat gcggcactaa atcttggcgc ccttcgcgcc gcatcatccg
      841 taggcaattt ttttcctctt tcaaccaata aagtaaccag ttttatcaaa acagaagtca
      901 aagcggtagc ctaattatgt ctgaccctat cgaacaaggt ttgggaccaa gtttcaaact
      961 gaccggtttt gttatagatt ctttttcttt ggaaaacgaa gcgcgccaag tagaaaatgt
     1021 agacgccgac atgttgcttt accatcacca aggaaatgca gaaatcatac cgcccaaaaa
     1081 gcttatccga atcaaacttt cggtaacggc ggagtatgaa gggaaaacat taggaaaaat
     1141 cgtgacgcaa acagaatttg aaatcatcaa ttttgaagtt ttcatcaaca acgaaatttt
     1201 aaatatccca ggagagctca tgtctatgct cgtcgggttg gcatactcta ctacgcgtgg
     1261 cgcacttatc gcaaaaagcg aaggtacggt tttagcaaaa tttcccatgc cggttgtttc
     1321 accctctcgt ctcatcggca ttgcaccgat gccgtcatga ccccgccgga gcgtactgtc
     1381 aaaaaaacgg tttcttcagt tcactttgtt ctactgtacc ctgcgcggat tagaacggat
     1441 gagcggagca aattcaaact attccggtaa ctccaccttt ccattcttaa aatcaagcgg
     1501 cggtaagaca accctaacac gattaaccgc accacttcag ataaggaatt ctcatgaaac
     1561 tcggcattat catagaaacc aaagaaccgg aaaaagcgtg gaatgcattt cgttttgccg
     1621 tcacatcccg caaaaccggc cacgaggtaa aagtatttct gatgggtgaa gccgtcgaat
     1681 gcgaaggact cacgcatgaa aaatacaacg tggatgaaca attgaaaaaa tttatcggtg
     1741 agggcggtac gatcttggcc tgcggtacat gcctcaaatc acggcaattg gatggtactg
     1801 aaagttgtcc gatttctacg atgaccgatt gtgttcagat ggtcgagtgg gcggataaag
     1861 tcgttacgtt ttaaaaatag aaatcctcaa atactacctc ggcatcgttt tgcaccgcat
     1921 cggcagcgaa gtttaccgtc gtgatgcgac tcgacgtaat cgcaggtgat ctcctctccc
     1981 acgcgaatca cacgtcgcgc ataaaactcg acgtgatgac cgtagacacg catccatgca
     2041 ttaggcgcac agctgtggtt gatccgcgca aaggcattgc catgatcaaa accgtctacg
     2101 gcccacccgt ccgaaaattc gaccaaagcg atagctccgc gttgtttagc acgccgacgg
     2161 gcttcacgta ccgtgatgat cgcgccggtc atttcaccga tcttacgccg tgccggtatt
     2221 ttttcatcgg caaaaacacc ccggccgtga atcgggcttt tttttatttt gactcggtat
     2281 ccgatcacgc cgtttgtaat atattttcct gcgacgaaac gacccgcggc ggcacgacgg
     2341 tataatgcaa atttttccac agcgctaccg gtgccgccaa aaactgaatc cgcatcattg
     2401 cgcggaaata tacaaatagc tgatgcaaga cgatcatcat ca
//
LOCUS       DAOQNQ010000414         2409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_26267_length_2409_cov_3.384452, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000414 DAOQNQ010000000
VERSION     DAOQNQ010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26267_length_2409_cov_3.384452"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PK754_15725"
     CDS             <1..284
                     /locus_tag="PK754_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11002.1"
                     /translation="ENWHPADHLARVQCPVFVAFGGQDEMFTAKHFEALTGVATKERD
                     EKLWLPEGHHRNLYEFELYRTSVTNFLTRYAKTNLYTLMAGEKTYASLP"
     gene            485..1174
                     /locus_tag="PK754_15730"
     CDS             485..1174
                     /locus_tag="PK754_15730"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNB11003.1"
                     /translation="MLPKAVADGFTRLLQPLIAFSSRTRINPNWFTTLGLLLNVVAAL
                     IFVAGSMYGTRSDLHYIGWGGFFVLMGGICDILDGKVARSTGLSSRFGALYDSVIDRY
                     SEVIMFLGMGYYLIGHNYFYESVFCFVALGGSTMVSYIRARSEGLQLECKVGLMQRPE
                     RVVWLGFGALFCGLSQYFVSPDFIFSIGSTHVFKPIYMFTGPIVIVAVLSNYTSVQRM
                     VHSYHQSKTSN"
     gene            1278..>2409
                     /locus_tag="PK754_15735"
     CDS             1278..>2409
                     /locus_tag="PK754_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HNB11004.1"
                     /translation="MGAVATTMVAGVIAVRKGLAQPVGSLTQLGTIRLGKRTEKRIPK
                     INEFVPLSGLEQIAFGGWDIFEDNAYQAAIKAGVIEKDLLNQIKPELEAVKPMKAVFD
                     QRYVKRLNGTHVKQGANKMELADMLRKDIQDFRKTNGCDRLVMVWCGSTEVFIKPTDV
                     HQTIEAFEKGLRENDINIPSSMIYAYAAIMEGVPYANGAPNLSADIPALVDLAQKKNV
                     PICGKDFKTGQTLMKTILAPGFKARLLGMNGWYSTNILGNRDGEVLDDPESFKTKEES
                     KLSVLEHILQPEVYPDLYGKISHVVRINYYPPRGDNKEGWDNIDIFGWLGYPMQIKVD
                     FLCRDSILAAPIVLDLALFLDLASRSGFKGIQEWLSFYFKSPQ"
BASE COUNT          652 a          504 c          544 g          709 t
ORIGIN      
        1 tggaaaattg gcaccctgcc gatcatttgg cgcgcgtcca atgccctgta tttgttgctt
       61 tcggaggaca agatgaaatg tttacggcta aacactttga agcccttacc ggcgtagcta
      121 ctaaagaacg tgatgaaaaa ctttggttgc ccgagggaca tcatcgcaat ttatatgaat
      181 ttgaattata tcgcacatcg gttaccaatt ttttgacccg atatgcaaaa accaatttat
      241 acacattaat ggctggagag aaaacatatg catctttgcc ttaagcgctg gttaaaacgc
      301 tggtttggca atatcttcag ttggtctttt tcgcaggatg atattgaaga cggattacct
      361 aatctgcatt ggcgtacgcc gaaaagctga gtttttagct tgatttttta ggcttcgatc
      421 aatactttgg gcgcgttatt tgaacattat ccgcacttca aattctgagg tttttttgag
      481 ttttatgctt cccaaagcgg tggccgacgg ctttacacgc cttttgcagc cattgattgc
      541 attttcaagc cgcacccgaa ttaatcccaa ttggttcacc acattaggac ttttgctcaa
      601 tgtagttgct gcacttatat tcgtagccgg atcaatgtac ggaacacgaa gtgatttaca
      661 ttatattggg tggggcggat ttttcgtttt gatgggcggt atttgcgata tcttggatgg
      721 caaagtggcc cgctctacgg ggctttcatc gcgatttggt gcattgtatg attcagtgat
      781 tgatcgctat agtgaagtga tcatgtttct cggtatggga tattacctga tcggacacaa
      841 ttatttttat gaatcagttt tttgttttgt agctttgggt ggttcgacga tggtcagcta
      901 catccgtgca cgatcggaag gtttgcaact tgaatgtaaa gtcggactta tgcaaaggcc
      961 cgaacgtgtc gtttggttag gttttggggc tttattttgt ggcctttctc agtactttgt
     1021 ttcaccggat tttatttttt ctattgggag tacacacgtt tttaaaccca tttatatgtt
     1081 caccggccct atcgtcatag tcgccgtgtt gtccaattat acgtccgtac aacgtatggt
     1141 tcacagttat catcaaagca aaacatcaaa ctgaatttga tatttgtttt agcataataa
     1201 gaaggagtct tgcattggaa aaaaagtctt tggatatcaa gcccgcatcg ggaaaactgg
     1261 gcgtcttatt accgggcatg ggcgctgtgg ccacgacgat ggtcgcgggc gtcattgccg
     1321 ttcgtaaagg tctcgctcaa ccagtcggat cactgaccca acttggaacg atacgcctcg
     1381 gcaaacgcac cgaaaaaaga atacctaaaa tcaacgagtt tgtaccgctt agcggtttgg
     1441 aacagatcgc tttcggcggt tgggatattt ttgaagacaa cgcctatcaa gcagcgatca
     1501 aagcgggtgt tatcgaaaaa gacttgctca atcagatcaa accggaatta gaagcggtaa
     1561 aaccgatgaa agccgttttt gatcagcgtt atgtcaaacg tctcaacggt acgcatgtca
     1621 aacaaggcgc caataaaatg gaattagccg acatgcttcg taaagatatc caagacttta
     1681 gaaaaacaaa cgggtgtgat cgtctcgtaa tggtatggtg cggttctaca gaagttttta
     1741 tcaagcctac cgatgtacat cagaccatcg aagcgtttga aaaaggatta cgagaaaacg
     1801 atatcaatat accctccagc atgatatacg cctacgcggc gatcatggaa ggtgtccctt
     1861 atgccaatgg cgcccccaat ctgtcagctg atatcccggc attggtggat cttgcacaga
     1921 aaaaaaatgt tcccatttgc ggaaaagatt tcaaaaccgg tcaaacactg atgaagacca
     1981 ttcttgcacc cggattcaaa gcccgtctgc ttggaatgaa cggctggtat tcaacaaata
     2041 ttctcggtaa tcgcgatggc gaagtacttg atgatccgga atccttcaaa actaaagaag
     2101 aaagtaaatt atccgtactt gagcacattt tgcaaccgga agtgtatccg gatctttacg
     2161 gaaaaatttc acacgttgta cgaatcaact attatccccc acgcggcgat aataaagaag
     2221 gatgggacaa cattgatatc tttggctggc tcggatatcc gatgcaaatc aaagtggact
     2281 ttctgtgccg tgactccata ttagcagcgc ccatcgtctt agaccttgct ttattccttg
     2341 atctggcatc tcgctccgga ttcaaaggta ttcaagaatg gctttcgttt tattttaaaa
     2401 gtcctcaac
//
LOCUS       DAOQNQ010000415         2405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_26338_length_2405_cov_4.583404, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000415 DAOQNQ010000000
VERSION     DAOQNQ010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26338_length_2405_cov_4.583404"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /gene="dnaE"
                     /locus_tag="PK754_15740"
     CDS             complement(<1..1228)
                     /gene="dnaE"
                     /locus_tag="PK754_15740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNB11005.1"
                     /translation="MSDFIHLHNHSDYSLLDSCCPVDDLVQAAEQHQMKALALTDYGN
                     LFGAAQFFSAAKAKNIKPIIGMEAYLAPNRLEKNKLIYHIVLFAKDQQGYQNLVALTS
                     QSYMDGFYYKPRIDKALLRAHAAGLIGTSACLKGEIPQRLMHGQYEEAKALALEYLEI
                     FQGDFYLEIQRHGLALDAVVNDGMQRLSRELNIPMIATNDVHYREKADAAAHEILLCI
                     QAGKTITDVTRPKYPGDTFYFRSPAEMKNLFKDVPSAIENTLALAEKISFKMEFGKSQ
                     LPVFGLPEGFTSENEYLAHLANAGLRKKMGETISEDAQKRLDYELAMIAKMGFPGYFL
                     IVQDFINASRQMGVAVGPGRGSAAGSLVSYAIGITDVDPMRYQLLFERFLNPERVSMP
                     DIDIDFDDRNRGKAIEY"
     gene            complement(1338..1414)
                     /locus_tag="PK754_15745"
     tRNA            complement(1338..1414)
                     /locus_tag="PK754_15745"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1378..1380),aa:His,seq:gtg)
     gene            1593..1994
                     /locus_tag="PK754_15750"
     CDS             1593..1994
                     /locus_tag="PK754_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11006.1"
                     /translation="MRPLYIINLILIALVSSCTYGYSASTDVTYSRSYAYKPHNRTVE
                     IYFGDNKPSRAYRQVGLIEVQGGQYYSMDALVAKLRRQAQAIGADAVMDIRKSSTQRE
                     RGSLLDALTDEKPEIYTAPVITGIAIKYIQP"
     gene            2010..>2405
                     /locus_tag="PK754_15755"
     CDS             2010..>2405
                     /locus_tag="PK754_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11007.1"
                     /translation="MRSLCLIITGYLFMSCVPPQIISNPAPKIAKSKGIIVACEFKTG
                     EVFEFADPGASVVSKGHVVFGRLESGDFTTYEKHQIAYLYTPEKTTWSDVDTTAKVTS
                     IELTDGFRVYFDNEGARIYSAGPCIVGSTL"
BASE COUNT          625 a          627 c          556 g          597 t
ORIGIN      
        1 cgtattcgat ggctttgccc cgattgcgat catcaaaatc aatatcaata tcgggcatgg
       61 aaacacgttc gggattgagg aaccgctcga acagcaactg atagcgcatg ggatccacgt
      121 cggtgatacc gatggcgtac gacacgaggc tgcccgcagc cgaaccgcgt cccggaccca
      181 ccgccacacc catctgacgc gacgcattga taaaatcctg cacgatgaga aaatatcccg
      241 ggaaacccat tttggcgatc atggccaatt cgtaatccaa ccgcttttgt gcatcttcgg
      301 aaatcgtttc gcccattttt ttgcgaagcc cggcgttggc caaatgcgcg aggtactcat
      361 tttccgacgt aaatccttcc ggcaatccga aaacgggcaa ctgggatttg ccaaattcca
      421 ttttaaagga aattttttcc gccagggcga gcgtgttttc gatcgccgaa ggaacatcct
      481 taaagagatt tttcatctct gcgggcgaac gaaaataaaa cgtatcaccg ggatacttgg
      541 gacgcgtcac atcggtgatg gttttacccg cctgaataca aagtagtatt tcatgcgccg
      601 cggcatccgc tttttcacgg taatgcacat cgttggtagc gatcatcgga atattcagtt
      661 ctcgggataa ccgctgcatg ccgtcattga ccaccgcatc gagcgccagt ccgtgccgct
      721 gaatttcgag ataaaaatcg ccttgaaata tctccagata ttccagcgcc aacgctttgg
      781 cctcctcgta ttgcccgtgc atcagacgct ggggaatttc tcctttgagg catgccgatg
      841 taccgatcaa tcccgcggcg tgcgcgcgaa gcagggcttt atcaatacgc ggtttataat
      901 aaaaaccgtc catataactc tgcgacgtca gcgcgactaa attttgataa ccctgctgat
      961 ctttggcaaa aagtacaatg tgatagatga gcttattttt ttctaatcgg ttaggcgcga
     1021 ggtaagcttc cataccgatg atgggcttga tgtttttagc tttggccgcg ctaaaaaatt
     1081 gggccgcgcc aaaaagattg ccgtaatcgg tcagcgccag cgctttcatc tgatgctgct
     1141 cggcggcttg tacaagatca tccaccggac aacaactgtc cagaagactg taatcggaat
     1201 gattatgaag atgtatgaag tcggacatcg ggtcaggatt aatttattgg gaagacaccg
     1261 taagacaaaa taaacaatcc cgccaaaaaa caaaatgcga agttgattca actccgcatt
     1321 ggatgggatc ttttcgatgg ggcgagcgac ggggctcgaa cccgcggcct tcagggccac
     1381 aacctgacgc tctaacctgc tgagctacgc ccgccatgaa acgtgccgaa gatagtgcac
     1441 cgcatttata tttgcaaggg gaaattgggt tttatcacag tgtattttaa tttcacatcc
     1501 gactcatccc aacacttcaa ataatttcaa cacgttaaaa aacggcactg actttgcatt
     1561 atcctccggc gttcacttaa accggagaat ttatgcgccc cctttacatc atcaacctga
     1621 ttttaatcgc gctcgtcagc agttgcacgt acggctactc ggcttcaacg gatgtgacct
     1681 attcccgctc ctacgcctac aaaccgcata accgcaccgt tgaaatttat ttcggcgata
     1741 ataaaccttc ccgtgcgtac cggcaggtcg gattgatcga agtgcaaggc ggccaatatt
     1801 acagtatgga tgcgttggtt gccaaattac gaagacaggc gcaggctatc ggcgccgatg
     1861 cggttatgga tattcgcaaa tcatccaccc agcgcgaacg cggcagtttg ctggatgccc
     1921 tgacggatga aaagcctgaa atctataccg cgcccgtgat cacgggtatc gcgatcaaat
     1981 atattcaacc ttaattcgcg gagtttttta tgcgtagctt atgcctcatc atcaccggtt
     2041 atttgtttat gtcatgcgta cccccgcaaa tcatttccaa tcccgctccc aaaatcgcaa
     2101 agagcaaagg catcatagtc gcttgtgaat ttaaaaccgg cgaggtattt gagttcgccg
     2161 accccggcgc atccgtcgtt tccaaagggc acgtcgtatt tggccgattg gaatccggag
     2221 actttacgac ttacgaaaaa catcaaatcg cttatctcta tacacctgaa aaaaccacat
     2281 ggtccgacgt ggatactacg gccaaagtca cttctatcga attaaccgac ggatttcgcg
     2341 tatattttga taacgaaggc gcacggatct acagcgccgg cccttgtatc gtcggtagta
     2401 cgctc
//
LOCUS       DAOQNQ010000416         2394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_26567_length_2394_cov_4.866182, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000416 DAOQNQ010000000
VERSION     DAOQNQ010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26567_length_2394_cov_4.866182"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="PK754_15760"
     CDS             <1..963
                     /locus_tag="PK754_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11008.1"
                     /translation="RLYRGIITLPAGNYVATFLTDDSHSFMDWNMQPPYDPSSWGMML
                     SVVDERDLDFVREYKKAKKQEILSITGVGDEQFRSEGLSLSRKTDLHIFALGEGRENR
                     MYDYGWIVNARDGNTIWEMRFRDTKFAGGADKNRLFDGVVSLPAGEYMVYYRTDGSHS
                     AEDWNDDPPYNQQKWGITLSLVNNSDASSISKLNELPEDNNILAQIVRVGDDEYETRD
                     FSLNRATEVRIQCIGEGRSGRMYDYGWIKNAETGQTVWKMLYEDTEPAGGARKNRSFN
                     GVVLLAPGNYKVTFISDDSHSYEDWNDDPPNLPDRWGITVRVNK"
     gene            complement(1035..>2394)
                     /locus_tag="PK754_15765"
     CDS             complement(1035..>2394)
                     /locus_tag="PK754_15765"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNB11009.1"
                     /translation="ALTEPEAGSDPAKLHTTAVESEDGQSYILNGEKLWCTNGTIADI
                     IVVMARHPEDGAITAFIVETKWPGVKVDHRCYFMGLKALENAVLSFKDVRVPKENIIW
                     GRGKGLKLALITLNTGRLTLPAGAAGTAKACLHVAREWASKRVQWGQPIGKHEAIAQK
                     IADMASMTFAMESVASLSTAMADKKLDIRLEAAVGKMYNTEAGWKIVDDTLQIRGGRG
                     YETADSLRARGEDPVAVERMMRDFRINLIFEGSSEIMRLFIAREAVDKHLEVAWKMID
                     PKSSIGEKLGALPKIALFYGIWYPTRWLGWGWWPRFSEFGPLATHMRFVERKTRKLSR
                     EIFHGMMRHQAKLAYKQAFLFRAVDIGAELFAMSATMSRAVKMAKEGNSDAMKLADLF
                     CRNARRRVEVSFKAMWNNDDNAKYNMAKDILNNKYGWMEAGIAESKESKTKSQSAPTV
                     AA"
BASE COUNT          616 a          589 c          555 g          634 t
ORIGIN      
        1 cgtttgtatc gcggcatcat caccttgccc gccggcaact atgttgcaac attcctaacg
       61 gatgattcgc attcgtttat ggattggaat atgcaaccgc cgtacgatcc ctcttcatgg
      121 ggtatgatgc tgagtgtcgt tgacgagcgc gatttggatt ttgtacgcga atacaaaaaa
      181 gccaagaaac aggaaattct ttctattacc ggtgtcggcg atgaacagtt ccgttctgaa
      241 ggcttatcgc tcagtcgtaa aacggatcta catatttttg ccctgggaga aggccgtgaa
      301 aaccggatgt acgactacgg atggatcgtc aatgcgcgcg acggcaacac aatatgggaa
      361 atgcgattcc gcgataccaa gttcgccggt ggcgccgaca aaaaccgttt atttgatgga
      421 gtcgtatcgt tacctgccgg tgaatatatg gtgtattacc gcaccgacgg ttcgcattcg
      481 gctgaagatt ggaacgatga tccgccatac aatcaacaaa aatggggcat tacgttaagc
      541 ctggttaata acagcgatgc atcgtccatc agtaaactta atgaattgcc ggaagataat
      601 aatatcctcg cacagatcgt tcgcgtcgga gatgacgaat acgaaacacg cgacttttcg
      661 ctgaatcgcg ctaccgaagt gcgcattcaa tgcatcggtg aaggacgttc cggaaggatg
      721 tacgattatg gatggataaa aaacgctgaa accggtcaga ctgtttggaa aatgctgtat
      781 gaagataccg agccggccgg tggcgcacga aaaaacagat catttaatgg tgtggtctta
      841 ttggctccgg gaaattataa ggtaactttt atttcggatg attcgcattc ctacgaagat
      901 tggaacgatg atccgccgaa tttaccggac cgctggggta ttacggtacg cgtcaataaa
      961 tagttttcct ctactgaaaa aataaaaccc tgagttgagc tcagggtttt ttattgccat
     1021 ttttataaat tgcctcaagc ggctacggtc ggtgcggatt gtgatttagt ttttgattct
     1081 ttggattccg cgatacccgc ttccatccaa ccgtatttat tattcaaaat atctttggcc
     1141 atgttgtatt tggcgttgtc atcattattc cacatggctt tgaatgacac ttcgacgcga
     1201 cgtcgtgcgt tacgacaaaa aagatccgca agtttcatcg catcggaatt accttctttg
     1261 gccatcttga cggcacgcga catcgtagcc gacatagcaa aaagttcggc gccgatatca
     1321 acggcgcgaa acaagaatgc ttgtttgtat gccaatttag cttgatggcg catcataccg
     1381 tggaaaatct cccgggataa tttgcgtgtt ttgcgttcga caaaacgcat gtgcgtcgcg
     1441 agcggaccaa attcactaaa acgtggccac catccccaac ctaaccaacg tgtcggatac
     1501 caaatgccat aaaacagcgc aatcttaggc agcgctccca gtttttcacc gatagaggat
     1561 ttgggatcaa tcattttcca agcaacttcc aaatgtttat ctacggcttc acgcgcaata
     1621 aacaagcgca tgatttcact cgatccttca aaaatcagat taattcgaaa atcacgcatc
     1681 atacgttcaa cggcgaccgg atcttcaccg cgtgcgcgta aagaatcggc cgtttcatat
     1741 ccgcggcctc cgcgtatctg cagcgtatca tctacaattt tccaaccggc ttcggtatta
     1801 tacattttac cgacagcggc ctcaaggcga atatccagct tcttatccgc catcgctgta
     1861 gataacgaag caaccgattc catggcaaac gtcatggatg ccatatcggc aattttttgt
     1921 gcgatggctt catgtttacc gatgggttgt ccccactgta cacgcttgga agcccattcc
     1981 cgcgctacat ggaggcatgc tttcgccgtt cccgccgctc ccgccggtag cgtcaatcgc
     2041 ccggtattga gcgtgattaa cgccaacttg agacctttac ctctacccca aatgatgttt
     2101 tctttgggca cgcgcacgtc tttgaatgac agaaccgcat tttccaatgc tttgagaccc
     2161 atgaagtaac agcggtgatc tacttttaca ccgggccatt tggtctcaac gataaacgcg
     2221 gtgatcgctc cgtcttcagg atggcgcgcc atcacaacga tgatatcagc gattgtaccg
     2281 ttggtacacc atagtttttc accattgagt atgtacgact gaccgtcttc gctttcaaca
     2341 gcggtggtgt gcaacttggc agggtccgaa ccggcttcag gttccgtcaa cgca
//
LOCUS       DAOQNQ010000417         2364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_27270_length_2364_cov_5.702036, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000417 DAOQNQ010000000
VERSION     DAOQNQ010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27270_length_2364_cov_5.702036"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1453
                     /locus_tag="PK754_15770"
     CDS             179..1453
                     /locus_tag="PK754_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019498761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HNB11010.1"
                     /translation="MHRHESIFALIDCNNFYVSCERAFQPKLEGKPVVVLSNNDGCIV
                     SRSNEAKALGVPMAAPYFEVKGLLDKHHAHVFSSNYVLYADMSHRVMQVLQRFTPDVE
                     VYSIDEAFIDLSGFTEDLTTYGIKIKETISRWTGIPVSVGIARTKTLAKIAAEKAKKS
                     KKLKGAFSLVNSPYLEKTLELTEVGDVWGLGRSSTAHLQSHGIYNALQFSKANPAWIK
                     KTMSIVGLRIAQELREVSCLPLAQVRERKKGITTSRSFSRAVTSLEEMTEAMATYVDR
                     CAKKLREEGAATKTLTVYLMTNRFTDDHYYYHKVVTLPVPSDDTGELIAYATSALRSI
                     FKEGLRYKKCGVMFTELIPKETVQTDMFDRISRERSSRRMEVLDAINARMGNGTLRYA
                     SEGWKKDWHMKCENRTPEYTTQWDQLLTVSAE"
     gene            1623..>2364
                     /locus_tag="PK754_15775"
     CDS             1623..>2364
                     /locus_tag="PK754_15775"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11011.1"
                     /translation="MRYLFVFFTFLLSMSAAYGQYTEYPTARYYHEKEITPREHTINI
                     ERMKLDVQFDPPKGLVKGKVTHVFKPLRASLDQLYFDGVNLRVTDVTINGKTAQFVNS
                     GQGITVFAKPALTWNDIRDSITFTYEANPRKGIYFVGWNDSTRRMRRQIWTQGEERDH
                     RYWIPMYDDPNDKVIHETSITFDKTYRVIANGKLQKEKDNKDGTKSWTYAMSQPHAPY
                     LLMLAIGQYEVKNLKSKSGVPIQLWYYPD"
BASE COUNT          687 a          544 c          555 g          578 t
ORIGIN      
        1 attggaattg gaattggaat tggaattgga attggaattg gaattggaat tattagagaa
       61 ttcccttccc cctctacatc gaatatacag aaaataattt ttgtgtcaag tcaaaattcg
      121 agttattatt aaacaccaag agctttttgt gtgaactcat caaggtcgta taagtatcat
      181 gcatcgccac gaatcaatat ttgccttaat tgattgtaac aatttttacg tttcgtgcga
      241 acgtgctttc cagcccaaat tggaaggtaa accggtcgtg gtgctttcca ataacgacgg
      301 gtgtatcgtg tcgcggtcca atgaagccaa agcgctcggt gtgccgatgg ccgcaccgta
      361 ttttgaagtc aaagggcttt tagataaaca tcatgcgcat gttttttcgt ccaactacgt
      421 cctatatgcc gacatgtcgc atcgtgtcat gcaagtgctt caacgattca cgcccgatgt
      481 ggaagtgtat tccatagacg aagcgttcat agatctcagc ggttttacgg aagatcttac
      541 gacgtacggc atcaagatca aggaaaccat ctcgcgttgg acgggtattc ccgtatccgt
      601 cggtatagcc cgtaccaaaa cattagccaa aatcgcagcg gaaaaagcca aaaaatcaaa
      661 aaaactcaaa ggggcgttca gcttggtgaa ttccccgtat ctggaaaaaa cgcttgaatt
      721 gaccgaagtc ggtgatgtat ggggcctcgg ccgcagctcg accgcgcatc tgcagtcgca
      781 cggcatttac aacgcgttgc aattttcgaa agccaatccc gcatggatca aaaaaacaat
      841 gtctatcgtc ggtctgcgca tcgcgcagga actgcgtgag gtatcgtgtt tgcctttggc
      901 gcaggtacgt gagcgcaaaa aaggcattac cacatcgcgt tcgtttagcc gggcggtcac
      961 atcgctggaa gagatgactg aagccatggc gacctacgtg gatcgctgcg cgaagaaact
     1021 gcgtgaagag ggcgccgcta cgaaaacgct gactgtgtat ctgatgacca atcgttttac
     1081 ggacgatcat tattattatc acaaagtcgt gacgttgcct gtaccgtcgg atgacaccgg
     1141 cgaactcatc gcgtatgcga catccgcatt gcgcagcatt ttcaaagaag gtcttcggta
     1201 taaaaaatgc ggcgtgatgt ttacggaact tattcccaaa gaaaccgtac aaacggacat
     1261 gtttgaccgc atatcgcgtg aacgctcctc ccgacgtatg gaagtattag atgccatcaa
     1321 tgcgcgtatg ggcaacggta cactgcgcta tgcgagcgaa ggatggaaaa aagattggca
     1381 catgaaatgc gaaaaccgta cgccggaata tacaacgcag tgggatcaac tattgacggt
     1441 cagcgcggaa taaattcgta tctgaagcgg cgttcatatc cataacgcaa aaatgccgcg
     1501 aatagcattt gcatatcaca tccgttggcg ttagttttta cggtcgtacg agaagatcat
     1561 atctttattt tcaaataaaa taaaactcaa atacctaaag ccaagtaaca aaggtcaaac
     1621 tgatgcgtta tctatttgta ttcttcacgt ttttattaag catgtccgcg gcgtatggac
     1681 aatataccga atatcccacg gcacgttatt atcacgaaaa agaaatcacg ccccgcgagc
     1741 atacgatcaa tatcgaacgt atgaaactgg acgtgcagtt tgatccgccc aaaggtctgg
     1801 tcaaaggcaa agtcacgcac gttttcaaac cgctgcgtgc atccttggat cagctatatt
     1861 ttgacggggt caatcttcgt gtaaccgacg tgacgatcaa cggcaaaaca gcccaatttg
     1921 tcaattccgg acaaggcatt accgtttttg ccaaaccggc cttgacatgg aacgatatcc
     1981 gggacagtat cacttttact tacgaagcca atccgcgtaa aggcatttat ttcgtcggat
     2041 ggaatgacag tacccgacgt atgcggcgtc agatctggac gcaaggtgag gaacgcgatc
     2101 atcgctactg gataccgatg tatgacgacc ccaacgataa agtgattcac gagacttcga
     2161 tcacgtttga taaaacctac cgcgtcatcg ctaacggtaa actgcaaaaa gaaaaagaca
     2221 acaaagacgg taccaagagt tggacgtatg ccatgagcca accgcatgcg ccgtatttgc
     2281 tgatgctggc gatcggacaa tatgaagtca aaaacctcaa atccaaaagc ggcgttccga
     2341 ttcagttgtg gtattatccc gatc
//
LOCUS       DAOQNQ010000418         2354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_27506_length_2354_cov_4.880383, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000418 DAOQNQ010000000
VERSION     DAOQNQ010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27506_length_2354_cov_4.880383"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..>2354
                     /locus_tag="PK754_15780"
     CDS             131..>2354
                     /locus_tag="PK754_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11012.1"
                     /translation="MTVSNGGRLYVGNDGRFGDIRFDNGGSFTITGSSTQVYAGRLVS
                     GATATSVNGLIDAAYVHIGYGATAAGTVYFMRGRSIHNIINGAVVNLGRDTVGDLTLN
                     QAPTGNVSSALHVDGSSTVFRINGEILAATANNPLKIDINGGTVYVGDSLDVLSTNPT
                     WVYMGAAGKADSLIIRGGGRFEFGRNVTTVNATGGNVTFYMRGNSLLDIQEGYLVLCG
                     NPALTSITAGNLFNMTENVTINVGNGSGPDSSAGIIVAPNLAAELPTPTTLNLFDLDG
                     TGNKMYVYSDGHVQVGGGNKSNMRLNGNANGLAGGPKLHMFGGKINVTGSLSLTTGDS
                     VYVQGGTLNIGLVASSGTNTITFAANDPGDTTAFIVDGGSVYVGDGNSAITMQTDNDN
                     IPSFDTGAVDAYNKLEIKNGIFYLNGRFQMADATARFDMSGGNFYVNPTGSQDLTGTN
                     SILLFQRGVVNFTGGTITLVNPNAYSGTGVALQVNGQATVGGTTVAANQLSGLPTALN
                     EVTDVVFQGTTSFGDGSASEDGSGDGFDITLADFGSSNPYGKFLVNSPSGSNRHVSLT
                     LQNSGGSHDSVLTVNDSLVIRAGEFRQNRNRIDYDGSGVAGMEIGYAGILRISSVQNF
                     DYFPQGFNSYVLDTVGSTVIYDGASSGTTVVDAGGSATFGNLTIAGNGTKQMNSSQRV
                     RGTLLLTSATLAAGSNLTMASGSTIKRDAGAMTASNNLGGNDYTIEYIGTNKNTAAGE
                     W"
BASE COUNT          599 a          448 c          613 g          694 t
ORIGIN      
        1 aaatacaaca gcgaatacaa cggttatatt caattctaca ttcgtggttg atgggggtat
       61 tgttcgcgtt tcacagggta caaatacagt cgcttttcaa agtttagtat ccggcagttc
      121 atcggcttcc ttgactgtat ctaatggcgg gcgtttatat gtgggtaatg atgggcgttt
      181 tggtgatatt cgttttgaca atggtggaag ttttactatt acgggatcgt cgactcaggt
      241 atatgctggt cgtcttgtgt ctggcgcaac agctacttct gtaaatgggc taatagatgc
      301 agcatacgtg catatcggat acggagctac tgcggctgga acggtttatt ttatgagagg
      361 gcgcagtatc cataatatta ttaacggagc cgttgtaaat ttgggccgcg atacagtcgg
      421 tgatttgacg ctgaaccaag ctccaacggg caatgtttca tcagctcttc atgtagatgg
      481 ctcgtctact gtatttcgga tcaatggtga aattttagct gcgactgcaa ataatccgtt
      541 aaaaatagac atcaatggcg gcacagtata tgttggtgat agtcttgatg ttttaagcac
      601 caacccaaca tgggtatata tgggcgctgc cggcaaagcg gattctttga ttattcgagg
      661 cggcggtaga ttcgaatttg ggcgaaatgt aaccactgtt aacgcaaccg gcggtaatgt
      721 aacgttttac atgcgaggta attctctctt agatattcaa gaaggctatt tggtattgtg
      781 cggcaatcca gcgctgacat ctataacggc aggcaacttg tttaatatga cggaaaatgt
      841 aactattaat gtcggtaatg gttctggtcc cgatagttct gcgggtatta ttgtagcgcc
      901 taatctggcg gctgagttac caacgcctac tacattgaat ttatttgatc tggatggtac
      961 gggaaataag atgtacgtgt attctgacgg acatgtgcag gttgggggtg gtaataagag
     1021 taatatgcgc ctcaatggca atgccaatgg tcttgccggg ggacccaaac tccacatgtt
     1081 tggcggtaaa ataaatgtca ccggctcttt atcgcttacg actggagatt cagtgtatgt
     1141 tcaaggtggc acactgaata tcgggcttgt tgctagtagc ggtacaaata cgatcacgtt
     1201 tgcggcaaat gatcctggcg atacgacggc atttattgtg gatggcggtt cagtctatgt
     1261 cggtgatggt aattcagcta ttactatgca gacagataat gataatattc cgagctttga
     1321 tacaggggca gttgatgcat ataacaagct tgaaatcaaa aacggaatct tttatctcaa
     1381 tggtcgtttc caaatggcgg acgctaccgc gcgtttcgat atgagtggcg gtaattttta
     1441 tgtaaatcca accggttcgc aggatttgac cggtacgaat tcaattcttt tattccaacg
     1501 tggcgtggtt aattttacgg gcggtacaat cacgcttgta aatccaaatg cttattcggg
     1561 taccggcgtg gcgttgcagg ttaatggaca agccacggtc ggagggacaa cggttgctgc
     1621 gaatcagctt tccggtttac ccacagcgct taatgaagtg actgatgtag tgtttcaagg
     1681 tacgacatca ttcggtgatg gtagcgcatc cgaagacggt tccggcgatg gttttgatat
     1741 tacccttgct gattttggaa gctccaatcc ttatggaaaa tttttagtaa atagtcctag
     1801 tggcagtaat cgccacgtgt cattgacgct tcagaattcc ggcggttcgc atgactcggt
     1861 actcacagtt aacgatagct tggttatacg tgccggtgaa tttcgtcaga atagaaatcg
     1921 tattgactat gacggatcag gtgtagccgg tatggaaatt ggttacgccg gtatattgcg
     1981 gattagtagt gtccaaaatt ttgattattt tcctcaaggt ttcaactctt atgtgcttga
     2041 tacggtaggt agtacggtga tatatgacgg cgcaagctcc ggtacgaccg ttgttgatgc
     2101 cggtgggtcc gcaacgtttg ggaatcttac aattgcaggt aatggaacca aacaaatgaa
     2161 ttcgtctcaa agggtccgcg ggacacttct tttgacttcg gcgaccttgg ctgctggttc
     2221 caatctgact atggccagtg gttcaacgat taagcgtgat gcgggtgcta tgaccgcttc
     2281 aaataacctc ggcggaaatg attatactat cgaatatata ggtacaaaca aaaacacggc
     2341 tgcgggtgaa tggt
//
LOCUS       DAOQNQ010000419         2343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_27764_length_2343_cov_6.347028, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000419 DAOQNQ010000000
VERSION     DAOQNQ010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27764_length_2343_cov_6.347028"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PK754_15785"
     CDS             complement(<1..610)
                     /locus_tag="PK754_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11013.1"
                     /translation="MMYRFFISPYLSSYLAFVFWATVYALSSVVSVAIVMPFVNLVFS
                     PHTPLTPLISFPVMDRTTALWILCGTTFAVFLIKNFAYIAFQSIGARLQQKLITALRE
                     FRIHELLRMSYGAFLAENHGAMHHRLITTSETTVRAWMQWIFSLCQHFPLLVLYSAVL
                     FILSWKITLLSLVLIPGITLLQHRLQKYTARWALFLHERESEI"
     gene            complement(610..>2343)
                     /locus_tag="PK754_15790"
     CDS             complement(610..>2343)
                     /locus_tag="PK754_15790"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNB11014.1"
                     /translation="NFEREWLYPAGFIDPENRWERETTNTLGMIFFILFSIMVVVLFF
                     RQFKRGGIDFKVGSFFGILSAVSFALMIGVQLLASGFWTLSFVVLIAGGWYFIMTTGI
                     IAVTASEVYRFWPESYRTFEAIRRGKIINRRWGRSLALGMAWTGITIGAISLVLYIVP
                     GTSFLLNSKSTDKYDGYGALFLIGASIWTVLIHHHLFYLLPLSALRKRWKNPAAVIGI
                     AALVGILQPYIFDTVSPWEPRLLAGLLIGIIYGTILLNYDFLTLSVSASLAYLISESG
                     FLYYTSDIPGMVIVGVYLCSFLTLALIALWSRDSGDDTLDYVPEYVKEMESKQRMNRE
                     FEIARTIQTKLLARTVPVNDRCDIACLCEPAHEVGGDYYDFVAFDDKNKLGVVIGDVA
                     GKGVSAAFYMTLVKGILQTQAPITSHSARETLERVNHIFCTQVERGRFISMVYGIFDF
                     SKNTVTIARAGHNPVYRLRSGASQMDSLVPDGMAIGLTQNDTFAEKLKETTIAFSSGD
                     VFVFFTDGFSEAMNKHGEEFGEARLQQTLLNVSGQNSSAMIDALQKEIRSFVGQHPQH
                     DDMTMIVIRIV"
BASE COUNT          707 a          541 c          560 g          535 t
ORIGIN      
        1 ggatttcgga ttcgcgctcg tgtaaaaaaa gcgcccatcg cgctgtgtat ttttgcaaac
       61 gatgttgcaa taacgtaatg cccggaatca atacaagact taataacgtg atcttccagc
      121 tgagtataaa aagcaccgcc gaatagagta caagtaaagg aaaatgctga caaagagaaa
      181 aaatccattg catccatgcg cgtaccgtgg tttcggacgt ggtgatcagg cggtgatgca
      241 tcgcgccgtg gttttctgcg agaaatgcgc cgtaagacat gcgcagcagt tcatgtattc
      301 gaaattcgcg aagcgccgtg ataagttttt gttgtaaccg tgcgccgatg gattggaatg
      361 cgatgtaggc gaagtttttt attaaaaaaa cagcaaaagt cgtcccgcat aatatccata
      421 aagcggtggt gcgatccatg acgggaaaag aaataagcgg tgtaagggga gtatgcggac
      481 tgaaaacgag attgacgaac ggcataacga tagccacact gacaaccgag ctcaacgcat
      541 atacggtagc ccaaaataca aaggctaaat acgatgacag gtaagggcta ataaaaaaac
      601 gatacatcat cacacgatac gaatcacgat catggtcata tcgtcatgct gcggatgctg
      661 tcccacaaaa ctacgaatct ccttttgcaa tgcatcaatc atggcagacg aattttggcc
      721 tgatacgttg agtaacgttt gttgaagtcg tgcctcgccg aattcttcac catgtttgtt
      781 catggcttcg gagaaaccat ccgtaaagaa cacaaaaaca tcgccggagg aaaaagctat
      841 cgtggtttct ttgagctttt ctgcaaatgt atcgttttgc gtcaagccga tcgccatgcc
      901 gtccggtacg agcgaatcca tctgtgaagc accgctgcgt aaacgataca cggggttatg
      961 accggcgcgt gcgatcgtga cggtgttttt ggagaaatca aaaataccat acaccatact
     1021 gataaaacgg ccgcgctcta cctgcgtgca aaaaatatga ttgacccgtt ctaaagtttc
     1081 acgcgccgaa tggctggtga tcggcgcttg tgtttgcaaa atgcctttga ccaatgtcat
     1141 ataaaaagcg gccgaaacgc ctttgccggc gacatccccg atgaccacgc caagtttatt
     1201 tttatcgtca aacgctacaa aatcataata gtcgccgccg acttcatggg cgggttcgca
     1261 tagacaagct atatcgcagc gatcgttgac cggtacggtg cgcgcgagca gtttggtctg
     1321 aatcgttcgt gcgatttcaa attcgcgatt catgcgctgc ttggattcca tttctttgac
     1381 gtattccggg acatagtcca gcgtatcgtc gccgctgtca cggctccaca gcgcgatgag
     1441 cgcgagtgtc agaaacgaac ataaatagac gccgacgatc accatgcccg gaatgtccga
     1501 tgtgtaatac aaaaaaccgg attcggaaat aaggtaggcc aggcttgctg atacggacag
     1561 cgtcagaaaa tcgtaattca ataaaatcgt accgtaaatg attcctatca ataaaccggc
     1621 aagaagacgc ggttcccacg gtgaaacggt atcgaaaatg tatggctgca aaatgccgac
     1681 caatgcagct atgccgatga cggcggccgg gtttttccag cgcttgcgaa gagcggacag
     1741 cggaagcaag taaaacaaat gatggtgtat gagaacggtc catatcgaag cgccgattaa
     1801 aaataatgcc ccgtatccgt cgtatttgtc cgtacttttg gagtttagta aaaacgaagt
     1861 gccgggtacg atgtataata caagtgaaat ggcgccgatc gtgatgcctg tccatgccat
     1921 accgagtgct aaactgcggc cccagcgacg attgattatt ttaccgcgac gaatagcttc
     1981 gaaagtacga taactttcag gccaaaagcg atatacttcc gaagcggtca cggcaataat
     2041 acctgtcgtc atgataaagt accaaccgcc ggcaattaac acgacaaagg aaagtgtcca
     2101 aaaaccggag gctagtagct gtacgccgat catcaatgca aaactgacgg cactgagaat
     2161 accaaaaaaa cttccgactt taaaatcaat accaccacgc ttgaactggc gaaaaaataa
     2221 cacaacgacc attatggaaa acaaaataaa aaagatcatg cccagcgtgt tggtcgtttc
     2281 acgttcccat cggttttccg gatcaataaa cccggctgga taaagccatt cgcgttcaaa
     2341 att
//
LOCUS       DAOQNQ010000420         2299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_28817_length_2299_cov_7.306595, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000420 DAOQNQ010000000
VERSION     DAOQNQ010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28817_length_2299_cov_7.306595"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2299)
                     /locus_tag="PK754_15795"
     CDS             complement(<1..>2299)
                     /locus_tag="PK754_15795"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNB11015.1"
                     /translation="SSASRFARYRVPDVTAPSTPTNLTASTGPNQLVLKWKKNSDVDF
                     SKYRIYFGSTSNPTVQTDSSLSISDTSRTMTGLTNGTTYYFRVSAVDIWGNQSSLSTQ
                     ASGIPNDTVPPSTPSLISATGGNLAVNLTWSKNNDSDFKRYRIYRGTSPGATTQVDSV
                     SIVGDTTKSISAPIPGVTFYFRVRTLDSANNLSTYSTELSASSLDQTPPSTITGLSTT
                     ASDTTVILRWRQSTATDFSKYYIYMDTLALPVTVVDSALTKADTARTFTHLTNGKTYY
                     FRVTAADTFANVSAFSSVASATPFDQTPPSAPQNLAAVASDASVVLHWDANTENDLLR
                     YRVFVGTSSGNEVQTDSTVYDSIVVNGLNNDTEYFFRIAAIDTGKNQSNYSNEVSATP
                     FAANAAPTNPVLLAATAQNQSVVLTWQKNPDTDILKYYVVAGLSSNPTALVDSTNGDT
                     TVTLSGLTNEQAYYFRIIAVDSALQVSGYSNELSQSPYDNVAPATPLNFIASAGDQSA
                     QLTWNNDNTEPIKIYRVFINGTQTDSLTDTTYTVTGLTNYTSYQVSVAAVDSNDNVSS
                     NTSDIGVTPIDLTAPSIPNNLTAEAGDASVHLVWSVNTESDLARYRIFINDVQSDSTS
                     DTSYTATALTNYTLYSFKIAAVDTSGNFSGFVSSTSMPYDQTAPAVPQDLAATAGDGQ
                     VTLNWTANGEGDFAHYRVYMDGTQIDSTTNNSYTKTGLSNYTTYNFKLSAVDTSRNTS
                     ALTSQVSVMPYDQTAPAVPQDLAATA"
BASE COUNT          631 a          507 c          529 g          632 t
ORIGIN      
        1 ccgccgttgc agccaagtcc tgaggaactg caggcgctgt ctgatcgtaa ggcataaccg
       61 atacttgtga agtcaatgcg cttgtattgc ggctggtatc aacggcagat aacttgaagt
      121 tatatgtcgt gtaattcgat aagccggtct ttgtatagct attgttcgtc gtcgaatcaa
      181 tctgtgtacc atccatataa acacgataat gtgcaaaatc accttcaccg tttgctgtcc
      241 agttcaatgt aacctgacca tcgcccgccg ttgcagccaa gtcctgagga actgcaggcg
      301 ctgtctgatc gtaaggcatt gacgtcgaag acacaaaacc cgaaaaatta cctgacgtat
      361 ctacggctgc tattttgaaa gaatacaatg tataattagt taaagcagtt gctgtataag
      421 acgtatcgct cgtcgaatcg ctttgtacgt cattgataaa aatacgatag cgagcgagat
      481 cactttccgt attaacagac catactagat gcaccgaggc atcgccggcc tcagccgtaa
      541 gattgttggg aatggaaggc gcagtcaaat caatcggggt tacgccgatg tctgaagtat
      601 tagatgaaac attatcatta ctatctacag cggcaacact aacttgatac gacgtataat
      661 ttgtcaaacc cgtcactgta tacgtggtgt ccgtaagtga atcagtttga gtaccattaa
      721 taaaaacacg ataaatttta ataggttccg tgttatcatt attccatgtg agttgcgccg
      781 attgatcacc tgccgatgcg ataaaattta acggtgtagc aggagcaaca ttatcataag
      841 gagattggga taattcattg gaatatccgc tgacctgcaa agctgaatca acagctatga
      901 ttctgaaata atacgcttgt tcattggtca atccggacaa tgtcactgtc gtatcaccat
      961 tggtagagtc caccaatgcc gtcggattag aagacaatcc tgcaacaacg taatatttca
     1021 atatgtcggt atccgggttt ttttgccacg tcaatactac cgattgattt tgagctgtag
     1081 ctgccaacaa aaccggattc gtcggtgcgg cattggctgc gaatggcgta gctgacactt
     1141 cattggaata attgctttga tttttgcctg tatctatcgc ggcgatccgg aagaagtatt
     1201 ccgtatcgtt attcaggcca ttcactacaa tactatcata cactgtagaa tcggtttgta
     1261 cttcattacc agaggaagtg cccacaaata cacgatacct caaaagatcg ttttccgtat
     1321 tggcatccca atgcaataca actgaagcat cacttgccac cgcggcaaga ttttgcggcg
     1381 cggaaggcgg tgtctgatca aaaggagtgg ctgacgcaac cgatgaaaat gccgatacgt
     1441 tagcaaatgt atcggccgcc gtcacgcgaa aataatacgt tttaccattg gtcaaatgcg
     1501 taaacgttcg tgcggtatct gcttttgtca gcgccgaatc caccaccgtt acaggcagcg
     1561 ccagcgtatc catatagata tagtatttac taaaatccgt cgctgtactt tgacgccagc
     1621 gcagaataac cgtcgtatca ctcgcagtcg tagatagccc cgtaatcgtg gatggtggcg
     1681 tttgatctaa ggaagatgct gataattcgg tggaatatgt gctcaggtta tttgctgaat
     1741 ctaaagtacg cactctgaag taaaatgtaa ctccgggtat cggcgctgaa atgcttttcg
     1801 tagtatcacc gacgatggat acagaatcca cctgggtcgt ggctccgggc gaagtacccc
     1861 ggtaaatgcg gtaacgtttg aaatctgaat cgttattttt tgaccaggta aggttaactg
     1921 ccaaattacc gccggttgct gaaataagcg aaggcgtaga tggcggcact gtatcgttcg
     1981 gtattccgct cgcttgtgtt gacaaactac tttgattacc ccaaatgtca acagcactaa
     2041 ccctgaaata gtacgtcgta ccgtttgtca aaccggtcat agttcttgag gtatcggata
     2101 tactgagcga agaatccgtt tgtacagttg ggttcgaagt gcttccaaaa tagattctgt
     2161 atttagagaa atctacatcc gaattttttt tccactttaa aacaagttgg ttcggacctg
     2221 tagatgcagt caggtttgtc ggcgtgcttg gcgctgtcac atccggcacg cggtatcgtg
     2281 caaaccgcga tgctgaaga
//
LOCUS       DAOQNQ010000421         2271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_29542_length_2271_cov_6.068141, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000421 DAOQNQ010000000
VERSION     DAOQNQ010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29542_length_2271_cov_6.068141"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PK754_15800"
     CDS             <1..334
                     /locus_tag="PK754_15800"
                     /inference="COORDINATES: protein motif:HMM:NF027650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter C-terminal domain-containing
                     protein"
                     /protein_id="HNB11016.1"
                     /translation="IRDFIGNYGEYREAERVKETVKDTSVEKTIVKDTNKKTKLSYKE
                     KIEFENLEKEIAALEKEKNELENKLNSGSADYIELQKWSERLSVISQTLDEKSMRWLE
                     LAEYTSAK"
     gene            complement(398..964)
                     /locus_tag="PK754_15805"
     CDS             complement(398..964)
                     /locus_tag="PK754_15805"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNB11017.1"
                     /translation="MIAKLFRWFFRMKGWTITAPIAPNIKKCVIIGAPHTSNWDFVYG
                     IGALECFNMRVNFLAKKELFRFPLKGMFLRMGGIPVDRSGKNSMVDAMIDVFKAREDL
                     MIIIPAEGTRKRVEKWKSGFYHLALGANVPIVLGFLDYEKKLAGFGPALYMTGDEIGD
                     MAKIRDFYKDKTGKIPEFFNIDAVRLSR"
     gene            complement(1018..1380)
                     /locus_tag="PK754_15810"
     CDS             complement(1018..1380)
                     /locus_tag="PK754_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11018.1"
                     /translation="MIFNISYVDVVFREKMSFMPKKSPVQSKKRVRVKGLGWKRSVAI
                     DSDKYKKVSKAILSVLTTSPIRFTEIVRLVEKKIPDFEGSLSWYTISVARELEAQGKI
                     IRHPKPVLYSKPRRTIQK"
     gene            complement(1377..>2271)
                     /locus_tag="PK754_15815"
     CDS             complement(1377..>2271)
                     /locus_tag="PK754_15815"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNB11019.1"
                     /translation="WGCAQKTPNPEKAPVLKKFSFISRQELQKFLTWSEGRSPLVSAH
                     RGGPVSGFPENCLATFENTIKYTYALIECDVRISKDGNLVMMHDKTLDRTTTGKGPVS
                     DMLFADLKQLRLKDNDGKITAYTIPTLDEVLVWAKGKTILMLDIKEGVTQVMIMEALR
                     RHEAFSYVVPITYNASEAASFIKLDSTLILSCSIEKEEDYHRLIAYGVKPRNIVAFCG
                     ISEPNKSLYQLLHAQNIFCQLGTMGNLDRKAVARGDTVYYELLNNGADVLSTDRHVEA
                     WSVIQHFLKDKKISDPYITFP"
BASE COUNT          686 a          510 c          435 g          640 t
ORIGIN      
        1 gattcgggac tttataggaa attatggcga gtatcgggaa gctgaacgcg taaaggaaac
       61 tgtcaaagat acctctgtag aaaaaactat cgtcaaggat acgaataaaa aaaccaaact
      121 ttcgtacaaa gaaaaaatcg aatttgaaaa ccttgaaaaa gaaattgcag ccttagaaaa
      181 agagaaaaac gaactggaga ataaactcaa tagcggttcc gcggattaca ttgaacttca
      241 aaaatggtct gaacgtttat ccgtcatttc gcaaacgttg gatgaaaaat caatgcgttg
      301 gttggaatta gccgaataca catccgccaa ataatcgcaa catattctcg acgccggatg
      361 cagtgaaaac gacgtattga ggtgcccgtc attacggcta acgtgataaa cgtacggcat
      421 caatattaaa aaattccgga atttttccgg ttttatcttt ataaaaatcc ctgatctttg
      481 ccatatcccc tatttcatca ccggtcatat atagtgccgg tccaaagcct gcgagcttct
      541 tttcataatc caaaaaaccc aatacgatcg gtacattggc accgagcgcc agatgataaa
      601 acccggactt ccatttttca acgcgcttac gggtaccttc cgcaggtatg atgatcatca
      661 aatcttcacg cgccttgaaa acatcaatca tcgcatccac catactgttt tttcccgagc
      721 gatccacagg aatgccgccc atacgcaaaa acatgccttt caacggaaat cgaaagagtt
      781 cttttttcgc aagaaaatta acacgcatat taaaacattc gagcgctcct ataccataca
      841 caaagtccca attactggta tgcggcgcac cgatgatcac gcattttttt atattcggag
      901 cgataggtgc tgtgatcgtc catcccttca ttcggaaaaa ccagcggaaa agcttagcga
      961 tcatattgtt tattacatca attagtatga aaaattaggt tcggaattta acgcgtatta
     1021 tttttgaata gtgcgccgag gttttgaata cagcacaggc ttcggatgtc ggataatttt
     1081 accttgcgct tcgagttcac gggcaacaga aatcgtatac cacgataacg agccctcaaa
     1141 atcgggaatc ttcttctcta caagccgtac tatttccgta aatcgtatag gactagtggt
     1201 caaaacggat aatatggctt tcgatacttt tttgtatttg tccgaatcaa tggccacgga
     1261 tcttttccaa ccgaggcctt ttacacgcac tcgttttttg gactgtactg ggcttttctt
     1321 tggcataaat gacattttct ctctgaatac gacatcaacg taagaaatat taaaaatcat
     1381 ggaaatgtga tatacggatc ggaaattttt ttatctttga gaaaatgctg aatcactgac
     1441 cacgcctcaa catgacggtc cgtagataac acatcggcac cgttatttaa taattcgtaa
     1501 tataccgtat caccgcgggc cacggctttt ctatcaaggt tacccatagt tccaagttgg
     1561 caaaaaatat tttgcgcatg taaaagctga tacaatgatt tattcggttc tgaaattcca
     1621 caaaacgcta ctatattcct tggtttcaca ccatatgcga tcaagcggtg ataatcttct
     1681 tctttctcaa tactgcatga taaaatcaac gtagaatcca gtttgataaa cgacgccgct
     1741 tcggaagcgt tataagttat gggaacgaca tagctgaatg cctcatgacg tcgaagcgct
     1801 tccatgatca ttacctgcgt gacaccttct tttatgtcaa gcatcaatat agtttttccc
     1861 ttagcccaaa ctaacacctc gtcaagcgta ggaatagtat atgccgtgat tttaccgtcg
     1921 ttgtctttta atcgcaattg cttcagatct gcaaaaagca tatccgatac agggcctttg
     1981 ccggtagtcg tgcgatctag tgttttgtcg tgcatcatga ccagattacc atctttactt
     2041 atacgcacat cacactcaat caacgcataa gtatacttga tggtattttc aaatgttgcc
     2101 aaacagttct caggaaaacc ggataccggg ccgccgcgat gagcgctcac gagcggagaa
     2161 cggccttccg accacgtaag gaatttttgt aattcttgcc gtgaaataaa ggaaaatttt
     2221 tttagaaccg gcgctttttc cgggttcggc gttttctgcg cacaccccca g
//
LOCUS       DAOQNQ010000422         2265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_29705_length_2265_cov_5.661991, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000422 DAOQNQ010000000
VERSION     DAOQNQ010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29705_length_2265_cov_5.661991"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..442
                     /gene="rpsJ"
                     /locus_tag="PK754_15820"
     CDS             122..442
                     /gene="rpsJ"
                     /locus_tag="PK754_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNB11020.1"
                     /translation="MTGQGIRIKLKAYDHNLIDKSIDKIIRTAKSSGAVISGPIPLPT
                     DKTIYTVLRSPHVDKKSREQFETRIHKRLICILNATNKTIEALSKLELPAGVDIELKV
                     TANS"
     gene            531..1169
                     /gene="rplC"
                     /locus_tag="PK754_15825"
     CDS             531..1169
                     /gene="rplC"
                     /locus_tag="PK754_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNB11021.1"
                     /translation="MSSGMIGRKIGMSNIFDESGNIIPVTLIEALPNVVTQIKTKEKD
                     GYEALQLAVGDKRGKRTNKAIQGHAKKANYTEKFPDVFREFDGFNVNDFTLGAEVKLD
                     IFAEGEKVTVIGISKGKGFQGVVKRHGFGGINETTHGASDRPRSPGSIGGSSDPSRVF
                     KGMRMAGRMGSDRVTIKNLKVVRLDKESNVLFIKGSVPGATNSYIEIRKNAK"
     gene            1202..1918
                     /gene="rplD"
                     /locus_tag="PK754_15830"
     CDS             1202..1918
                     /gene="rplD"
                     /locus_tag="PK754_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNB11022.1"
                     /translation="MKIEVKNIKGEATGRSVELPKDIFGIEPSEHAVYVAVTHELSNK
                     RHGTHSTLEKSEVSGTTKKPWKQKGTGGARSGSVKTPLWPGGGIVFGPRPHLYKKDTN
                     VKLKRLARKSVLSIKASENRIVVVEDFHTLCADLPKTKEVYGVLNNLQLTGEVTVDNG
                     KRTKPFQKVLVLVDKTGLVKDEKSAKVYENFRKSCRNIPGLKVGVVNNVSTYELMNAD
                     TILFHESAIKNVNAAFGAAE"
     gene            1942..>2265
                     /gene="rplW"
                     /locus_tag="PK754_15835"
     CDS             1942..>2265
                     /gene="rplW"
                     /locus_tag="PK754_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNB11023.1"
                     /translation="MNITQINKILIRPVITEKMTILQDKKKRNGEPLNQYGFIVEKTA
                     TKTDIKKAIEKHFNVKVKSVRTVNVEGKSSVRYTKRGFVQGKRADRKKAIVTLAKDNK
                     IEFVEG"
BASE COUNT          714 a          497 c          558 g          496 t
ORIGIN      
        1 gtggtcgtac cgtaggcgcc ggtgtagtaa ccaagatcct caagtaagaa gaaaagattg
       61 accgcgggtg tcgcccaaag caatgcccgc ggaaaaataa aattaacgag agaatgcaaa
      121 agtgactgga caaggaatcc gaatcaaact gaaagcttac gatcacaatc tgattgataa
      181 gtcgattgat aagattattc gcacagcgaa aagctccggc gccgtgatta gcggcccgat
      241 tccgctccct accgacaaga cgatttatac ggttcttcgt tcgccgcacg tggataaaaa
      301 gtcccgcgaa cagttcgaaa cgcgtattca caagcgattg atctgtatct tgaatgcgac
      361 gaataaaacg atagaagcgt tgtccaaact cgaattgccg gcaggcgtgg acattgaatt
      421 gaaggtcact gccaatagct gatcacttga tcaagttaat attggctaaa gtttagatat
      481 caatttggat ggaacaggaa aacatcctgt ttcttacgag gagtatatac atgagcagcg
      541 gtatgatcgg aagaaaaatc ggaatgagca atatcttcga cgagagcggg aacattatcc
      601 cggtgacgtt gatcgaagcg cttccgaacg tcgtgacgca gatcaagacc aaagaaaaag
      661 acggttatga agcgttgcag ctggctgtcg gcgacaagcg cggcaagcgt accaacaaag
      721 ccatacaagg ccacgccaag aaagccaact acacggaaaa atttcctgac gtgtttcgtg
      781 aatttgacgg cttcaatgtc aatgatttca cgctcggcgc ggaagtaaaa ctggacattt
      841 ttgccgaagg cgaaaaagtc accgtgatcg gcatttccaa aggtaaaggt tttcagggtg
      901 ttgtaaaacg ccatggcttc ggcggtatca atgaaaccac acacggcgcc tcggatcgcc
      961 cccgttcacc gggttcgatc ggcggtagct cggatccttc ccgtgttttc aaaggcatgc
     1021 gcatggccgg ccgtatgggt agtgatcgcg tgaccatcaa aaacctgaag gtcgttcgtc
     1081 tggataaaga atccaacgta ctctttatca agggtagcgt gccgggtgcg acgaattcgt
     1141 acattgagat tcgcaaaaac gcgaagtaag aagttaacat ttttgataga aggctctggc
     1201 aatgaagatt gaagtcaaaa atatcaaagg cgaagcgacc ggtcgcagcg tcgaattacc
     1261 gaaagacatt ttcggaatcg aacccagcga acatgcggtt tatgttgccg tgacgcacga
     1321 actttcgaat aaacgtcatg gtacacatag cacgcttgaa aaatcggaag tttccggtac
     1381 caccaaaaag ccgtggaaac aaaaagggac gggcggcgcc cgctccgggt ccgtcaaaac
     1441 cccgctttgg ccgggcggcg gtatcgtgtt cggtccgcgt ccacatcttt ataaaaaaga
     1501 cacgaatgtc aaactcaaac gtttggctcg taagagtgta ttgagtatca aagcatcgga
     1561 aaatcgcatt gtggtggttg aagattttca taccctttgc gccgatcttc cgaagaccaa
     1621 agaagtctat ggcgttttaa ataatctgca attgaccggt gaagtgaccg tagataacgg
     1681 caaacgtact aaaccgtttc aaaaagtact cgtgcttgtt gataaaacag gtcttgtcaa
     1741 agatgaaaaa agcgctaagg tgtacgaaaa cttccgcaaa tcatgccgta atattcccgg
     1801 acttaaagtc ggcgtggtga acaatgtttc gacgtacgaa ctgatgaatg cggataccat
     1861 tctttttcac gagagtgcga tcaaaaatgt caatgccgca ttcggcgctg cagaataaac
     1921 gataaggact agagactgat catgaatatc acgcaaatca acaaaatcct gattcgtccg
     1981 gtgattaccg agaagatgac gattctgcag gacaaaaaga aacgtaacgg cgaaccgttg
     2041 aatcaatacg gtttcatcgt cgaaaaaacc gctacgaaaa ccgatatcaa aaaagcgatc
     2101 gaaaaacatt ttaacgtcaa agtgaaatcc gttcgtacgg tcaacgtcga aggaaagagc
     2161 agcgtacgtt ataccaaacg cggttttgtc caaggcaaac gtgccgatcg taaaaaagcg
     2221 atagtaacat tggccaaaga caacaaaatc gaatttgtcg aaggc
//
LOCUS       DAOQNQ010000423         2264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_29726_length_2264_cov_6.365776, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000423 DAOQNQ010000000
VERSION     DAOQNQ010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29726_length_2264_cov_6.365776"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="PK754_15840"
     CDS             <1..851
                     /locus_tag="PK754_15840"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB11024.1"
                     /translation="GVNTSFTTIRNWGMMHGAMFTDQDYRSGNKVCVLGSTVAVNLFG
                     QSDPTGEIVRIQNIPFRVAGVLESKGQLASGQDQDDMIIIPYTTYDKRLTGDKNIDYI
                     YVSVRTSQDMTTAMEEIRQILRQEHRIPAYGDDDFTIRTQEQVNEIAKSISKTVSLLM
                     IIVASISLLVGGIGIMNIMLVSVTERTREIGIRMAIGATEKHILLQFLVEAVVLSLTG
                     GVIGMILGVVIGYLVETFTSWAVSISMVSVVVSMSFAAGVGIFFGFYPARKAASLNPI
                     EALRFE"
     gene            975..1652
                     /locus_tag="PK754_15845"
     CDS             975..1652
                     /locus_tag="PK754_15845"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNB11025.1"
                     /translation="MPSDKSGSVETIITDVEIIYIFDTLCGWCYGFGPVVQRLRENYS
                     REIPFTIMSGGLAVGDRVKPIGYMSHYILDVMPRLETMTGVTFGKPYRDLVEQGQFVL
                     DSLPPAVALAVCKKTKPEMAFDFASEVQSLHFMSGADTNQPDFYANVAKQFGLDGNKF
                     ILAINDPVWRETAQREFRDVHYLGIQGYPALVFRAGDQAYLIANGYTDFDALDLRLKS
                     ILRTVNT"
     gene            complement(1642..>2264)
                     /locus_tag="PK754_15850"
     CDS             complement(1642..>2264)
                     /locus_tag="PK754_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11026.1"
                     /translation="DGCSDEDGDACSTESEYYETEDDTEDDGCSDEEDDSCESSDEES
                     DDSDYSDDDGCSDDEGCESDDDTYSSDDSDYYEDDGCSCQGETVVYSDDTPENYYNST
                     LRVHFSVYTGGADWTIRIEDNYGKTLYTMMRVDNVATGNYSYNWSGDYYDDAIGQSRF
                     ILPGSYYVVLQRRDGGIYQSKKSFTYNSKNGFQVDGLNINEINFMY"
BASE COUNT          591 a          481 c          523 g          669 t
ORIGIN      
        1 atggcgtcaa tacaagtttt acgaccatac gcaattgggg tatgatgcat ggcgcgatgt
       61 ttacggatca ggattatcgt tccggaaaca aagtctgtgt acttggctca accgtggcag
      121 tcaatctttt cgggcaaagc gatccgacag gggaaatcgt tcgtatccag aatatcccgt
      181 ttcgtgttgc cggtgtactc gaatcgaaag gtcaattggc gtccggccaa gatcaggacg
      241 atatgatcat cattccatat acgacttatg ataaacgact gacgggcgac aaaaatattg
      301 attatatcta tgtttccgtg cgaacaagtc aggacatgac tacagcgatg gaggaaattc
      361 gtcaaatttt gcgtcaggag catcgtattc ctgcatacgg cgatgatgat tttacgatac
      421 gcacacagga acaagtcaat gaaattgcca agtctatttc caaaaccgta tcgctactca
      481 tgattatcgt agcgtcgatt tcactattgg taggcggtat aggcattatg aatatcatgt
      541 tggtttcggt aacggaacga acacgtgaaa tcggcattcg tatggcgatc ggtgcaacgg
      601 aaaaacacat tttgttacag tttcttgtcg aagccgtagt actgagtctt accggaggcg
      661 tgatcggcat gatattgggt gtcgtgatcg gttatttggt tgagacattt acgagttggg
      721 ccgtcagtat ttcgatggtt tccgtagttg tttcaatgtc tttcgccgcc ggtgtcggca
      781 ttttctttgg tttttatcct gcccgaaaag ccgcgtcgtt aaacccgata gaggcattgc
      841 gtttcgagta agtattaatt ttctcaaaac tgcggcatat atgtaaatag tgttcgatct
      901 ccaatttatt ttgaaataac aaaccatcgc gaaagcgttg gtttttttta taacaatacc
      961 attggtgcaa ttctatgcct tcagacaaat ccggttcagt agaaacgatc atcacagatg
     1021 tggaaatcat ttatattttt gatacgctgt gtggttggtg ctacggtttc ggcccggtcg
     1081 ttcaacgact aagggaaaac tattccaggg aaataccgtt cacgatcatg agcggaggat
     1141 tggctgtggg cgatagagtg aagcctatcg gctacatgtc gcattatatt ttagacgtga
     1201 tgccgaggtt agagactatg acgggtgtta cattcgggaa accgtatcgt gatttggttg
     1261 aacaaggaca gtttgtattg gattcgttgc ctcctgcagt agcgctggcc gtatgtaaaa
     1321 agacaaagcc tgaaatggca tttgattttg cgtccgaggt tcaatcactt cattttatga
     1381 gcggagccga taccaatcaa ccggattttt atgcaaacgt tgcaaaacaa ttcggattag
     1441 acggaaataa atttattctt gcgattaatg atccggtgtg gcgtgagacc gcgcaacgag
     1501 aatttaggga cgtacattat ttaggtattc aagggtatcc ggcactggta tttcgtgccg
     1561 gagatcaagc ttatctgata gccaatggat ataccgattt tgatgcgctt gatcttcggc
     1621 ttaaaagcat actgcgaaca gttaatacat aaagttgatt tcattgatat tcaggccgtc
     1681 cacctgaaat ccgttttttg aattataggt gaatgatttt ttactttggt agataccgcc
     1741 gtcccttcgc tggagtacga cgtaataact tcccggcagg ataaaacgac tttgtccgat
     1801 ggcgtcatcg taataatcac cgctccagtt gtacgaataa tttccggtcg ccacattatc
     1861 tactcgcatc atcgtataca acgttttccc gtagttatct tcgatgcgaa tcgtccaatc
     1921 cgcgccccct gtatagactg agaaatgtac gcgcagagtg ctattataat aattctcagg
     1981 cgtatcatcg gagtatacca cagtttcacc ttggcaggag catccatcgt cttcataata
     2041 atcggaatca tccgaagagt atgtatcgtc gtcggattcg cacccttcgt catcggaaca
     2101 tccgtcatca tccgaataat cgctgtcatc ggattcctca tcagaagatt cgcagctatc
     2161 gtcttcttca tcactgcagc catcatcctc tgtatcgtcc tcggtctcgt agtattcgga
     2221 ctcagtcgaa caggcatctc catcttcatc ggaacatccg tcat
//
LOCUS       DAOQNQ010000424         2261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_29814_length_2261_cov_5.721668, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000424 DAOQNQ010000000
VERSION     DAOQNQ010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29814_length_2261_cov_5.721668"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PK754_15855"
     CDS             <1..605
                     /locus_tag="PK754_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HNB11027.1"
                     /translation="QIALFVGAIASSAMIGWTVQILDTPTSEMAAQGIQHAIGTEKYP
                     APQGTLMATLIKGLLSFNLDWQFVLVGAFVAVTMELCGVKSLSFAVGAYLPLSTTLPI
                     FAGGAIKGIADWNRSRKGESEEDAELGKGSLFATGLVAGGALFGVIVAIATVFAEDFM
                     KSINMEHTFSGILGQAGYEIFGVICFALMGITLYRVAMKK"
     gene            827..1576
                     /locus_tag="PK754_15860"
     CDS             827..1576
                     /locus_tag="PK754_15860"
                     /inference="COORDINATES: protein motif:HMM:NF012571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine-4-hydroxylase"
                     /protein_id="HNB11028.1"
                     /translation="MTLENENHTTEELEVWRLLFERQSSLLHNAACQTFLEGLTVLDF
                     QPDAIPHLKSINAKLRDMTGWSLTVVPGIVEDTIFFEMLSRNEFPVTGWLRSKKQLQY
                     IEEPDMFHDVFGHVPMLTDKQFCGFLEGLSRIALAYPNNTFIIERLVRMYWFTVEFGL
                     VRESDNVKICGAGILSSIGETLYCLSGKPPVVEFDLHTVFEMPFRKDRFQDRYVMISD
                     FEQLFRSVYQMEETLLRLQRQIQLQVLSWSC"
     gene            1648..>2261
                     /locus_tag="PK754_15865"
     CDS             1648..>2261
                     /locus_tag="PK754_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11029.1"
                     /translation="MNSANKSNEIERIADMLRLEMNNRPSEDFIKSFIRQIDSLAPNV
                     LRYFRSTLSQTLIERENQGKGHLKIEALLELLDLCDLQVWLTSAGLHEKAARNVVKGL
                     KNRSTEELNRIIHGKLYIEHDRTELLEFLVDAGVEGDDAEHFVKLVREMDVSVTEIID
                     RIFKLNFVAENAFRQESLRGMMSLKEIVTLYLYSVLQVTISDYV"
BASE COUNT          610 a          476 c          515 g          660 t
ORIGIN      
        1 agcagattgc gctttttgtc ggtgcgattg ccagctccgc gatgatcgga tggacggtgc
       61 aaatccttga tacgccgact tctgaaatgg ccgcacaagg tattcagcat gcgatcggca
      121 cggaaaaata cccggcaccc cagggaacat tgatggctac cttgatcaag ggtttgttgt
      181 cttttaacct tgattggcag tttgttcttg tcggcgcatt tgtagctgtg acaatggaac
      241 tctgcggcgt caaatcgtta tcgtttgctg taggcgcata cttacctctt tcgacgacac
      301 ttcctatttt tgccggcggc gccatcaaag gtattgccga ttggaatcgc tcacgtaagg
      361 gtgaatcgga agaagatgcg gaacttggca aaggcagtct ttttgcgaca ggtcttgttg
      421 ccggcggcgc gctctttggc gtcatcgttg ccatcgcaac tgtgtttgcc gaagacttca
      481 tgaaatccat caatatggaa catactttct ccggtatttt gggccaggcc ggttatgaaa
      541 ttttcggtgt tatctgtttt gctctcatgg gcattacact gtaccgggtt gcaatgaaaa
      601 agtaagtgcc gtgaactgtt tttgacttta gagagaacat tacttattat ttgatatatg
      661 agaacagcgc ctatacatat agaaacatcg ttttattatt acttttatcg caacggatcc
      721 ccgctttgga gatgcgatta aggtagtttt tatttttaat tcaccaaccc gtttccccaa
      781 aggaaacggg ttttttattt tcacccttta atgaaaggaa aacgccatga ctttggaaaa
      841 tgaaaaccac acaacagaag aactcgaagt ttggagactt ttgttcgaac gccaatcgtc
      901 actgctgcac aacgcagcgt gccaaacgtt tttggaagga ctcaccgtat tggattttca
      961 accggatgcc attcctcatt tgaaatcaat caatgcaaag cttcgggata tgaccggttg
     1021 gtcgctcacg gtagtacccg gcatcgtcga ggatacgatt ttttttgaaa tgctgtcgcg
     1081 taacgaattt cccgttacgg gatggttgcg atcaaaaaaa caacttcagt atatcgagga
     1141 gccggatatg tttcatgatg tattcggaca cgtccccatg ttaaccgata aacaattttg
     1201 cggtttcttg gaaggattga gccgcatcgc cttagcctat cccaacaaca cctttattat
     1261 tgaacgcctc gtacgtatgt attggtttac ggtcgaattc ggtttggtac gtgaatccga
     1321 taacgtcaag atttgcggcg ccggaatcct ttcatccatc ggcgaaacgc tgtattgcct
     1381 cagcggcaaa ccacccgttg tggagttcga tttacacacc gtatttgaaa tgcccttccg
     1441 aaaggatcgt tttcaagatc ggtacgtgat gatcagtgat tttgagcaat tatttcgatc
     1501 cgtgtatcaa atggaagaaa ctttgttacg tttgcaaaga caaattcaac ttcaggtttt
     1561 atcatggagc tgttgagcga taaaactgaa aacaattgaa aaacacgttt ttatggctaa
     1621 atttagcaca ttcaaaaaag gttacggatg aattcagcca ataaatcaaa cgaaatcgaa
     1681 cgcattgcgg acatgcttcg actggagatg aacaatcgtc ccagtgaaga ttttattaaa
     1741 tcgtttattc gccaaataga cagccttgcg cccaatgtat tacggtattt tcgctctacg
     1801 ctcagtcaga ctttgatcga acgcgaaaat caaggcaaag gacatcttaa aatcgaagcg
     1861 cttcttgagt tattggattt atgcgattta caggtgtggc ttacttcggc aggactgcat
     1921 gaaaaagccg cccgtaatgt cgtcaaagga ttaaaaaacc gatccactga agaactcaat
     1981 agaatcattc acggcaagct ctacatcgaa catgatcgca cggaattact tgagttcttg
     2041 gttgatgccg gtgttgaagg cgatgatgcc gaacattttg tgaaacttgt tcgtgagatg
     2101 gatgtgtccg taacggaaat tattgaccgc atcttcaaac ttaattttgt tgccgaaaat
     2161 gcgttccgac aagaatccct gcgtggtatg atgtcgctga aagaaattgt gacgttatac
     2221 ttatactcgg ttttacaagt tacgatcagt gactatgtgc a
//
LOCUS       DAOQNQ010000425         2255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_29961_length_2255_cov_4.999091, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000425 DAOQNQ010000000
VERSION     DAOQNQ010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29961_length_2255_cov_4.999091"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PK754_15870"
     CDS             complement(<1..440)
                     /locus_tag="PK754_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11030.1"
                     /translation="MNGFSVWAADKSGVIRKDETWEGVITVTGDVTIPEGITVRIRPN
                     TLIRFMANQDDEAGGIDVAKSELIVEGILRAEGQEKFQIRFTSSNFTDPGEKGSTLQP
                     QAGDWYGLIFRRGEDRSILSNCVIEFAYDGITCINSSPRIFRNR"
     gene            complement(493..>2255)
                     /locus_tag="PK754_15875"
     CDS             complement(493..>2255)
                     /locus_tag="PK754_15875"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNB11031.1"
                     /translation="KAEKVEPNEKDTKDSTLQIKEETRTQSVTPTKTILPGVEEKPKF
                     VAEAVVQNKIEVKPVEEIKKATPDKKTDDRQSTLASPVTDDTKTIEIEVPKALLAMMA
                     AGDADDELNGLRIPSFAERLNTEPKDIITGGRRTVKKKRSELPKEQVKPIPEKASVKE
                     ALKTMTVAPPLKTEKVESAVVEGKLMLEESAFDAVDPEILDIFNQEAAEYFKKFDAAF
                     ARLKQSLVDDVAIRDLERTSHSLKSSARMLGFERISGLSAAVELITERCVEKEISVDA
                     TIVTLLESITTALRLIAARKAADVTQVVQQLFELENSLNASGTFTGAIPGSASLAKHK
                     GIETKAPEKKPEPVIEAVKPAEVSQPAPKENYFSKLGIDDEIVDIFKEEVSTYFKLIG
                     AALSTLRNNMTHRQSMRDIEKAAHSLRSSAKMLGFQKISNLVKPMETVAEKMNEGELR
                     VTSELIDLYETSIERLKQLTAGTDVDVDDVVGKLQSYEQKAPEATDIKPVNDEDVRAR
                     IKAELTQEIKLDKVPEKAVTPNEKVISEKKTETKVKAVKKPAKAFDDVPIEKDAILKN
                     LLKSGPQLLDEMFEATQHGV"
BASE COUNT          505 a          567 c          481 g          702 t
ORIGIN      
        1 atgcggtttc gaaatatacg cggcgaggaa ttgatgcaag tgataccgtc gtaagcaaat
       61 tcaatcacgc agttcgataa aatactgcgg tcttcacctc gacgaaatat taaaccgtac
      121 cagtcgcccg cctgaggttg aagtgtgctt cctttttcgc ccggatcggt aaaattgctg
      181 gaggtaaacc gtatttgaaa tttttcctga ccttcggctc gtaaaatacc ttccacaatc
      241 agttcggact ttgcaacgtc aatccctccg gcttcatcat cctggttcgc cataaatcgg
      301 atcaacgtat tggggcgtat gcgcacggta atgccttccg gtatcgtgac atctccggta
      361 acggtaatca cgccctccca tgtttcgtct tttcgaatga cgccggattt gtccgcagcc
      421 cacactgaaa aaccgttcac cagtacaatc gtgagcagta tccgcagcct gcacctatgg
      481 agtttaaaga aattatacgc catgctgggt tgcctcaaac atttcatcca ataattgggg
      541 tccactcttg agcaaatttt tcaaaatcgc atctttttca atcggtacat catcaaaagc
      601 ttttgccggt ttttttaccg cttttacctt ggtttccgtt ttcttttctg aaattacttt
      661 ttcattcgga gtcaccgctt tttccggcac tttatctaac ttaatctctt gtgtaagctc
      721 cgctttgata cgcgcacgga cgtcctcgtc attgacgggt ttgatatcgg tggcttcggg
      781 cgctttttgt tcataactct gaagttttcc cacgacatcg tccacatcaa catccgtacc
      841 ggccgtaagc tgtttaaggc gttcaatact cgtctcgtaa aggtcaatca gttccgatgt
      901 gacacgtaat tcaccctcat tcattttttc ggcgaccgtt tccatcggtt tgacgaggtt
      961 acttatcttt tggaaaccca gcatttttgc cgaactgcga aggctatgcg ccgctttttc
     1021 gatgtcgcgc atggattgcc gatgcgtcat attatttcgc agcgtactca gcgcagctcc
     1081 gataagcttg aagtaggtgg atacttcttc tttaaagata tcaacgatct cgtcgtcaat
     1141 tccaagtttc gaaaagtaat tttctttggg agcaggttga gaaacctcgg ccggtttgac
     1201 agcttcgata acaggttcgg gtttcttctc cggcgcttta gtttcaatgc ccttatgttt
     1261 ggctaatgat gcgctgccgg gaatggcgcc tgtaaaagta cctgaggcat taagcgaatt
     1321 ttccagttca aacaactgct gtactacctg cgttacatcc gccgcttttc gggccgcgat
     1381 caaacgcaga gctgtagtga tagactccaa taacgtgact atcgtcgcat caaccgatat
     1441 ttctttttca acacaccgtt ccgtgatgag ttctacggcc gccgataaac cgctgatacg
     1501 ttcaaatcca agcatacgcg ccgaactctt gagactatgg ctggtgcgct ccagatcgcg
     1561 aatggccaca tcgtcaacta aagattgctt cagacgggcg aacgccgcat caaacttttt
     1621 gaaatattca gcggcctctt gattaaaaat atccaaaatt tcaggatcca ccgcatcaaa
     1681 ggccgattct tccaacatca gttttccttc gacaaccgcc gattcgactt tttcggtttt
     1741 caaaggcggc gctaccgtca ttgtcttgag cgcttccttg accgatgctt tttcgggaat
     1801 aggcttcacc tgttctttgg gaagctcgct tctttttttc ttgacggtac ggcgaccgcc
     1861 cgtgatgata tctttgggtt ccgtattaag gcgttcggca aaagaaggga tacgcaaccc
     1921 gttcaattca tcatcggcat caccggcggc catcatagct aataaggctt tgggaacttc
     1981 gatttctatc gtcttagtat catccgtgac cggcgaagcc agtgtggact ggcgatcatc
     2041 tgttttttta tcaggcgttg ctttttttat ctcttcgacc ggttttactt cgatcttgtt
     2101 ttgtacaacg gcttcagcga caaatttagg tttctcttca acccccggca aaatagtctt
     2161 tgtaggcgtt acactttgtg tacgcgtttc ttctttgatt tgtaaagtcg aatccttggt
     2221 gtctttttca ttgggttcaa ctttttccgc tttga
//
LOCUS       DAOQNQ010000426         2250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_30093_length_2250_cov_5.276993, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000426 DAOQNQ010000000
VERSION     DAOQNQ010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30093_length_2250_cov_5.276993"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1638)
                     /gene="lon"
                     /locus_tag="PK754_15880"
     CDS             complement(<1..1638)
                     /gene="lon"
                     /locus_tag="PK754_15880"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNB11032.1"
                     /translation="MTKTLEEQISTKEKKKRGGQVPDSLPIIPLRNTVLFPQQIMPLS
                     IGRDKTLRLVSEFADANRLIGVVAQKESAIENPSQTEMYEYGVAATIVRVFDMPDGSK
                     SVIVQGVQRIRINDFIQEDPYWIANVTELGDIPAPASDLEIDALMNNIKNIFQKMSGL
                     APYITAEQTTMILNIQQPGRLSDAAVAALNIPTDEKQGILQILDVKIRLEQTHIMLTK
                     VLQTLELGNKIQSDVQDEISKTQREYYLREQLKAIQRELGEVETGNPEIAELREKVEK
                     ANMPKEALEVAKKELERLGRMSPMAAEYTVSRTYIDWLIDIPWNIFTEDNLNIKEAHE
                     RLDSDHFGLDKVKKRILEYLAVRKLKNDMRGPILCFVGPPGVGKTSLGRSIANALGRK
                     FVRVALGGIRDEAEVRGHRRTYIGALPGRVLQGIKKAGSSNPVFMLDEIDKVGMDFRG
                     DPSSALLEVLDPEQNNSFSDHYLEVPFDLSKVLFIATANLADPIIPALRDRMEVIEIP
                     GYTEEEKIKIAEKFIIPKQTKEHGLKENQIRFTKDAVR"
     gene            complement(1716..>2250)
                     /locus_tag="PK754_15885"
     CDS             complement(1716..>2250)
                     /locus_tag="PK754_15885"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNB11033.1"
                     /translation="TFDEENDAPLAGDVITKVNGKSVENSAEIRKNIVNKNPGTSVDL
                     GVIRDGKDMTIKVVLGEAPSEQPVAASKEESTTAKKNAERLGMGIANLSRDLAQKYGF
                     NAKEGGVVITSIDQNGPAFRGGLREGDVIKRVGRTDIKSTKEFSEAVNKISKGDTALF
                     LVNRKGTSMFIAFNIPN"
BASE COUNT          539 a          542 c          475 g          694 t
ORIGIN      
        1 tcgtacggca tctttggtaa aacgaatttg gttttctttc agaccgtgtt ctttggtttg
       61 ttttggaatg ataaattttt cagcgatctt aattttttct tcttccgtgt aacccggaat
      121 ttctatgact tccatacgat cgcgaagcgc cggtatgatc gggtcagcca aattggccgt
      181 agcaataaaa agtactttac tcaaatcaaa aggtacttcg aggtaatgat cggaaaacga
      241 attattctgc tccggatcca aaacttccag taatgcactt gacggatcac cccgaaaatc
      301 cattcctacc ttatcaattt cgtccagcat gaaaacagga ttggatgaac cggctttttt
      361 tattccttgt aatacacgac cgggcaaggc gccgatatag gtacgacgat gacctcgtac
      421 ctctgcctca tcgcgaatac ctcccaaagc gacgcgtaca aatttgcgtc caagagcatt
      481 ggcaatagag cgccctagtg atgttttgcc tacgcccgga gggccaacaa aacataaaat
      541 cggtccgcgc atatcgttct taagtttacg aacagcaagg tattcaagaa tacgtttttt
      601 aaccttatcc aatccaaagt gatccgaatc taacctctcg tgcgcttctt tgatattgag
      661 gttatcttcc gtaaaaatat tccacgggat atcaatcagc caatcaatat aggtgcgcga
      721 aaccgtgtat tcggccgcca tcggcgacat gcggccgaga cgttctaatt cttttttagc
      781 tacctccaat gcctctttag gcatgttggc cttttctact ttttcgcgta attctgcgat
      841 ttccggatta ccggtttcga cttcgcccag ctcgcgctgt atggctttga gttgttcacg
      901 caaataatat tcgcgttgcg ttttactgat ctcgtcttga acatcgctct gaattttatt
      961 accaagttcc aaagtctgaa gtactttggt aagcatgata tgcgtctgct ccagtcggat
     1021 tttgacatcc aaaatttgaa gtataccctg cttttcatcc gtaggaatat tgagcgctgc
     1081 tacggcggca tcggacaatc gtccgggttg ttgaatattg aggatcatcg tcgtctgctc
     1141 ggccgtgata tatggcgcga gccccgacat cttttgaaaa atgtttttga tgttattcat
     1201 caacgcgtca atttccagat cgcttgccgg tgcaggaata tcacccagct cggttacatt
     1261 ggcaatccag tacggatctt cctgtataaa atcgttaatg cgtatgcgct gaacaccctg
     1321 cacgatgacg cttttgcttc cgtccggcat atcaaaaaca cgtacgatcg tcgcggccac
     1381 accgtattcg tacatttcgg tttgagacgg attttcgata gcgctttctt tttgagccac
     1441 gacaccgatc agacgattgg catcggcgaa ttcagacacc aaacgtaatg ttttatcacg
     1501 tccgatcgac aacggcatga tttgctgcgg gaacagcacc gtgttgcgca gcggaatgat
     1561 cggcagcgaa tcgggcacct ggccgccgcg ttttttcttt tctttggtgg aaatttgttc
     1621 ttccaacgtt ttcgtcataa ccttcctggt tgattagaaa aaaagcatca ggaaaagtcc
     1681 tgatgctttt ctctaagatc ggaaatttta attttttaat tcggaatatt aaatgcgata
     1741 aacatcgaag ttcctttgcg attgacgagg aacaacgcgg tatcgccttt actgattttg
     1801 tttacagctt cagaaaattc tttagtggat ttgatatccg tacgacctac gcgtttgata
     1861 acatcacctt cgcgcagacc acctcggaat gccggaccgt tttggtcaat cgatgtgatc
     1921 acgacgccgc cttctttggc attaaatccg tatttctgcg ccaaatcgcg actgaggtta
     1981 gcaatgccca tgcccaatct ttcagcattt ttctttgcgg tcgtactttc ttctttgctg
     2041 gccgctacgg gttgttctga cggcgcttcg ccgagtacaa ccttaatcgt catatctttc
     2101 ccatcacgta taacgccgag atcaaccgat gttccggggt ttttgtttac gatatttttt
     2161 cgaatttcag cactgttctc aaccgatttg ccgttaactt tggtgattac atcgcctgct
     2221 aatggcgcat cgttttcttc gtcgaatgta
//
LOCUS       DAOQNQ010000427         2235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_30507_length_2235_cov_4.950917, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000427 DAOQNQ010000000
VERSION     DAOQNQ010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30507_length_2235_cov_4.950917"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..521)
                     /locus_tag="PK754_15890"
     CDS             complement(72..521)
                     /locus_tag="PK754_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11034.1"
                     /translation="MRRRIAGNIGFLSILLTMVSCTSYIPFSYKGYDNKTQYSTNIGS
                     TMIYRESGYKKATAPDTGRTTDPLQINTIHHGSRYELIYAGINHNVIQILYREYVVRI
                     NQGYYEPVFSQIFQYDINENKSITFQNYKIQVFEANSELIRFQVLSD"
     gene            727..1077
                     /locus_tag="PK754_15895"
     CDS             727..1077
                     /locus_tag="PK754_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNB11035.1"
                     /translation="MQSYMMIFRGGDDAMVKMSPDEIQQHMGQWFAWVEKLTKEGRYV
                     SGEPLVMEGKVVRAKGVITDGPFAEGKELVGGFFIVKAKSIEEACDMAKVCPSFQFDG
                     ATVEIRQVQEMEHA"
     gene            1087..>2235
                     /locus_tag="PK754_15900"
     CDS             1087..>2235
                     /locus_tag="PK754_15900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB11036.1"
                     /translation="MSVGENHPSIESLVDHLFRNSAGKMVSVLTRLFGSHNITLAEDV
                     VQDTLLKAMQIWPRAGIPQNPEGWLMQVAKNRALDLIRRRSREIELPEDTDPLLSSAW
                     TRATRMEYLFLPHEIRDDQLRLMFTCCHPMLPQESRIALTLKTLCGLGTKEIARAFLT
                     SEETIQKRLYRAKQLLREHRIRFEIPDDGISERFNSVLHVIYLLFNEGYNTTRTDAVI
                     RQDLMDESIRLGEMLLQNNRTNTPATQALLALMYLHAARTDARTDATGHILLLAEQER
                     RLWNQTMILKGLTHLHASARGMTITRYHLEAGIAGCHATAQSFAATNWDRIVQLYDLL
                     LQTQDHPVIRLQRGIALCQSQSPQTALAWLQQQGIDEALADYPYLHATY"
BASE COUNT          593 a          542 c          526 g          574 t
ORIGIN      
        1 cgcaaaggtt acaagacatt ttttgtacgt tcatttctta gcgtcttagc gagaacgcat
       61 ttcgcaccat cttagtctga taaaacctga aatcgaataa gttcggagtt cgcttcgaat
      121 acctgaattt tgtaattttg aaacgtgatg gatttgtttt cgttgatgtc atattgaaat
      181 atctgactga ataccggttc ataataccct tgattgatgc gaacgacgta ttcacgatag
      241 agtatctgaa taacattgtg attaataccc gcataaatga gctcgtaacg tgaaccgtga
      301 tgaatggtat tgatctgtag cggatctgtc gtcctaccgg tatccggtgc tgtggctttt
      361 ttgtatccgc tctcccggta gatcatcgta ctaccgatat tcgtagagta ttgagtttta
      421 ttatcatatc ctttatagga aaaagggata taagaagtgc atgataccat ggttaacaaa
      481 attgataaaa aaccgatatt tcctgcgata cgacgacgca tagatgattt cccttcacga
      541 tttggttgtt gttgttggat taacataaat tcaccaaaaa tcgcaaggta aacattgtta
      601 tttaagcatt tacaatgtat gccgtaaaaa tttataaaat tttttaactc catgtccgat
      661 ttccgatccg tcattcgtca ttaatatata gtacatttaa tacataccaa cgaaagggta
      721 caacgtatgc aatcgtacat gatgattttc cgcggtggcg atgacgccat ggtcaaaatg
      781 tcaccggatg agatccagca gcacatgggc cagtggtttg cttgggtgga aaaactgacc
      841 aaagaaggac gttatgtcag cggcgaaccg ctggttatgg aaggcaaagt cgtccgcgcc
      901 aaaggcgtca tcacggacgg accttttgct gaagggaaag aattggtcgg cggattcttt
      961 attgtcaaag ccaaatccat cgaagaagca tgcgacatgg ccaaagtttg ccccagcttt
     1021 cagtttgacg gcgccacggt cgagatacgc caggttcagg aaatggagca cgcataataa
     1081 cccagcatga gtgtcggcga aaatcatcca tccatagagt ctttggtaga ccatcttttt
     1141 cgtaattcgg cgggaaagat ggtctccgtt ttgacgcggt tattcggcag tcataatatc
     1201 acgttggccg aagacgtcgt gcaggatacg ctgctcaagg cgatgcaaat ctggccgcgc
     1261 gccggtatcc cgcaaaatcc cgaaggctgg cttatgcagg tagccaaaaa ccgtgcgctg
     1321 gatctgatcc gtcgccgcag ccgcgaaatc gaattaccgg aagataccga cccgctgtta
     1381 tccagtgcat ggactcgtgc aacgcgtatg gaatatcttt ttctgccgca cgaaatccgc
     1441 gacgaccaac tccgcctgat gtttacctgc tgccacccga tgttgccaca ggaaagccgc
     1501 atcgctctga cgctcaaaac gctgtgcggt ctgggcacca aagaaatcgc acgcgcgttc
     1561 ctgacttccg aagaaacgat tcaaaaacga ctataccgcg ccaaacagct tttgcgggaa
     1621 catcgtattc ggtttgaaat ccccgatgac gggatatcgg aacgcttcaa ctcggttttg
     1681 catgtgatct atctgctgtt taatgaaggc tataatacga cccgcaccga tgcggtgatt
     1741 cgccaagatc tgatggacga atcaatacgg ttgggggaaa tgcttttaca aaataatcgc
     1801 accaataccc ccgccacgca ggcattgcta gctctgatgt acctgcacgc cgcacgtacc
     1861 gatgcgcgta ccgacgccac cggccatatt cttttgttgg ccgaacaaga acgccgtttg
     1921 tggaatcaaa caatgattct caaaggcctt acgcatctgc acgcctcggc acgcggcatg
     1981 acgatcacac ggtatcatct ggaagccggt atcgccggtt gccatgccac ggcacaaagt
     2041 tttgccgcga ccaattggga tcgtatcgtg cagttgtatg atctgctttt gcaaacgcag
     2101 gatcatcccg tgatccgact ccagcgaggc atcgcacttt gccaatccca atcgccccaa
     2161 acggcgctcg cctggcttca gcagcaaggc attgacgagg cactggccga ttatccgtat
     2221 ttgcatgcca cgtac
//
LOCUS       DAOQNQ010000428         2232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_30583_length_2232_cov_6.743225, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000428 DAOQNQ010000000
VERSION     DAOQNQ010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30583_length_2232_cov_6.743225"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..489
                     /locus_tag="PK754_15905"
     CDS             247..489
                     /locus_tag="PK754_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11037.1"
                     /translation="MARLTASEWVARENNCSEPEAEVMIEDDPDLEYDYYIRYCLQEL
                     NLSPDEIIRGFNSDMTEAQERFIEDFKGEDEMELEQ"
     gene            673..2040
                     /locus_tag="PK754_15910"
     CDS             673..2040
                     /locus_tag="PK754_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11038.1"
                     /translation="MRLRHRLFIIAVMMYAINAWAQDGATTTPQDSAVAATPQETPLD
                     STQTLTPKLTELFTKLRTDSNNAALLYDIYKEYLKIGRRNYAVEFINASLRKDPTNMD
                     VLYEKGELLLEMGKKKSAYEAFLLVMRDFKGQAYLDRIGPKFVSNFKITQITNNKFND
                     VMASYAPDGKSIIFQSDRSGNWDIFTMNPDQGESSVKKLTDDLEADENPAYSTDGKWI
                     AFASTRDDKSTKEYKSRELYFMDKTGKRVTRLTSSYGGDNWSPIFLDTVTIAFSSDRS
                     DFSSTPFAKKNTGIYTIERNGSFLFKMFYQDKMNYTDVAYKAVSNQFVFAQRADDSDF
                     DLYMTSTDTKEKPVNLTNHPKNDLQPSVSKNGNFIAFVSDRDGNYEVYRMQADGRDQT
                     RITNDDGDDVYPKFSPDGNKIIFSSNRNGKYQLYIASTEESSAVTVQSVIAILEKRIA
                     SSSDN"
BASE COUNT          654 a          457 c          512 g          609 t
ORIGIN      
        1 gatgagttaa tgaaagctcg cgcacaaggg taccgcatcg cggattggag ttttgataca
       61 gatgggttaa ccgttgaaac tgtagcggag tcggtcttac aatatatcac caaacggcgc
      121 ggcattgaaa aaaagtgagt ttgaccttga caaaccatgc ctgtgttatt ataattcgcc
      181 gaatttgaaa cgttggaaat aatttatttt atcatattca cttaacacgt acgcaaagga
      241 gctgttatgg cgagattaac cgcgtcggag tgggtcgcac gcgaaaataa ttgttcggaa
      301 ccggaagccg aagtcatgat cgaagacgat ccggatctgg aatacgatta ttatatccgc
      361 tactgcctgc aagaactcaa tctttcgccc gatgaaatca ttcgcgggtt caattcggat
      421 atgaccgagg cgcaggaacg cttcatcgaa gacttcaaag gcgaagatga aatggaactt
      481 gagcaataat aatctcgggt atacaatcta agtttgaaaa ggagccgcgc acaacgttcg
      541 gctcctttta tttttaagag gttatcaggg gaaaaaccgc ttgcatttat aggggctttt
      601 ggctaaaatg cgggcaatta taatacagat tatccgttta ccaactcccg aatttccagg
      661 aggatttttt tcatgcggtt acgccatagg ttattcatta tcgccgttat gatgtatgcg
      721 atcaacgcgt gggcacaaga tggcgctaca acaacgcctc aggattccgc tgtagctgcg
      781 acaccgcagg aaacgccgct ggattcgacg caaacactga cgcccaaatt gacggagctt
      841 tttaccaagc tgcgcaccga cagcaataat gccgctttac tgtatgatat ttataaagag
      901 tatctgaaaa taggtcgccg caattatgcc gtcgaattca tcaatgcgtc gcttcgcaaa
      961 gatccgacca acatggatgt cttgtacgaa aagggcgaat tgcttttgga aatgggaaag
     1021 aaaaaatcgg cgtatgaagc atttctgctg gtgatgcgtg atttcaaagg gcaggcgtat
     1081 ttggatcgta tcggccctaa atttgtcagt aatttcaaaa ttacacagat aacgaataac
     1141 aaatttaatg acgtcatggc gtcctacgcg ccggacggaa aatctattat ttttcaatcc
     1201 gatcgcagcg gtaattggga tatcttcacg atgaatccgg atcagggaga gtcctctgtc
     1261 aaaaaactga cagatgacct ggaagccgat gaaaatccgg cttattctac cgatggtaaa
     1321 tggatcgcgt ttgcctccac gcgggatgat aaaagtacca aagaatataa gtcgcgcgaa
     1381 ctgtacttca tggataaaac cggaaaacgt gttacccgac tgacctcgag ttatggtggt
     1441 gataactgga gtccgatttt cttagatacc gtgacgattg ctttttccag tgatcgttcg
     1501 gatttctcgt ctacaccgtt tgcaaagaag aatacgggca tttacacgat tgaaagaaac
     1561 ggcagcttcc tcttcaaaat gttttatcag gacaagatga attatacgga tgttgcgtac
     1621 aaggcagtat cgaaccaatt cgtatttgca cagcgtgccg acgattctga ttttgatctt
     1681 tacatgacca gcacggatac taaagaaaag ccggtcaatc ttacaaatca tccgaagaac
     1741 gatctgcagc cgtccgtttc aaagaacgga aactttatag cttttgtgtc ggatcgtgat
     1801 ggtaattacg aagtgtaccg tatgcaagcc gacgggcgcg atcaaacccg tattaccaat
     1861 gacgacggag atgacgtgta tcctaaattt tcacccgacg gcaacaaaat tattttttcc
     1921 tcgaaccgta atggtaagta ccagctttat atcgcttcga cggaagagtc ttctgccgtt
     1981 acggtgcagt cggtgatcgc tatacttgaa aaaagaattg catcgtcatc ggacaattga
     2041 tgcttaggtg atcggcgaca aaccatcggc aatagatgat tttgatataa agttcgttct
     2101 aaagctatct gttattagag atagtgaaat gggttttgta acacggcggg atttttaagt
     2161 atttaaagta acgccgtgtt attctctcat aactcccatc ctttttttaa atttgtttac
     2221 aattattttt ta
//
LOCUS       DAOQNQ010000429         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_30699_length_2228_cov_4.212609, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000429 DAOQNQ010000000
VERSION     DAOQNQ010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30699_length_2228_cov_4.212609"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PK754_15915"
     CDS             <1..365
                     /locus_tag="PK754_15915"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNB11039.1"
                     /translation="MEAGVEFRYLLYKARDPNEAILGMDLAAFADYGKVWERYDFKNN
                     SYNGKRLPAMPVAAVGLGTRIRTLIGPLRVDVGYKLQDVSKLKVLNASGTAVEAITRA
                     NAKKISPIALQVTLGQAF"
     gene            365..754
                     /locus_tag="PK754_15920"
     CDS             365..754
                     /locus_tag="PK754_15920"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNB11040.1"
                     /translation="MDTKNPFDRKKFLPLIFWMAVIFILSSVPGKEYPSYDIPHLDKV
                     VHFGLYGVLGYAAWFAFAAYGQTKGKSASWMAGVAASVALIYAVSDELHQLYVPFRSC
                     DAADLIADAMGIGAVVGWKYFTFKDAR"
     gene            756..1679
                     /gene="ftsY"
                     /locus_tag="PK754_15925"
     CDS             756..1679
                     /gene="ftsY"
                     /locus_tag="PK754_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNB11041.1"
                     /translation="MLSLLGKLKEKLTKTRQNLFGRISAVLQGKTILDEALLSEIEEI
                     LITADIGVQTAHKILERTRQRIKKEKISEAGQIYRLLEEEIENLIIPVAGKPLEEKMS
                     DKKPFVLLIVGVNGTGKTTTIGKLANRFAQAGKKVLMVAGDTFRAAASNQLEIWARRS
                     DVDIIRQNDGADPSSVIFDAMQSSATGKYDVVLIDTAGRLHTKINLMEELKKMRRVIE
                     KHIPGAPHETILVLDGTTGQNAIQQARQFSQAVQVSGLILTKLDGTAKGGVVLAIQQE
                     LKIPVLYIGVGEGIDDLEAFDPHAFIQAIVE"
     gene            1722..>2228
                     /locus_tag="PK754_15930"
     CDS             1722..>2228
                     /locus_tag="PK754_15930"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNB11042.1"
                     /translation="MKKIRLILGIHNHQPVGNFDFVLDKAYKNAYRPFLDMLRDFPGV
                     RVGMHTSGILYEWLDKHYPEYSIQLRTLTDRGQIELIGGGFYEPILPIIPHRDQVGQI
                     QRLQKYLLQRYEQAAQGMWLAERVWEPHLPSVLSQVGVRYVILDDTHFLHAGLSVDDL
                     SGYYITEDQ"
BASE COUNT          585 a          502 c          575 g          566 t
ORIGIN      
        1 ttatggaggc gggagtagaa tttcgctact tactctataa agcgcgcgat cccaatgaag
       61 ccattctcgg aatggacctt gccgcgtttg ccgactacgg taaagtatgg gagcgctacg
      121 acttcaaaaa taattcctac aacggaaaac gattgccggc tatgcctgta gcggccgttg
      181 gcctcgggac gcgcattcgc acgctcatcg ggccgttgcg agtggatgta ggctacaaac
      241 ttcaggacgt cagcaaactc aaagtcctca atgcgtcggg aaccgccgtg gaagctataa
      301 cccgtgccaa cgccaaaaaa atctctccca tcgctttgca ggtgacgtta gggcaggcgt
      361 tttgatggat acgaaaaatc ctttcgatcg taaaaaattt ttgccgctga ttttttggat
      421 ggccgtgatt tttattttgt cgtctgttcc cggaaaagaa tatccgtcgt atgatatacc
      481 gcacttggat aaagtcgtac actttggttt gtacggtgtt ttaggatacg ccgcgtggtt
      541 tgcgtttgcg gcgtatggac aaactaaagg caaatcggct tcatggatgg ccggggttgc
      601 cgcgtcagtg gcgttgatct atgcggtttc ggacgaattg catcagctgt acgttccttt
      661 cagatcatgc gatgcagccg atcttatcgc cgatgctatg ggtatcggtg cggtagtcgg
      721 atggaaatat tttacattta aagatgcaag gtagcatgct ttcactactc ggtaaactca
      781 aagaaaaact aaccaaaacg cgccaaaatc tttttggccg catcagcgcc gtactccagg
      841 gaaaaacgat tttagatgaa gccttgctgt cagaaatcga agaaattctc ataacggccg
      901 atatcggagt tcagacggca cataaaattt tagaacgtac gcgtcaacgc attaaaaagg
      961 aaaaaatcag cgaagccggt caaatttaca gattattgga agaagaaatc gaaaatttga
     1021 ttatccccgt agccggtaaa ccgctcgaag aaaaaatgtc cgacaaaaaa ccgtttgttc
     1081 ttttgatcgt cggtgtcaac ggtacaggca agacgacgac catcggtaaa ttggccaatc
     1141 ggtttgcaca ggccgggaaa aaagtgctca tggtggccgg cgatacgttt cgtgcggcag
     1201 cgagtaatca acttgaaatc tgggcccgcc gctccgacgt ggatatcatt cgccaaaacg
     1261 acggcgcaga cccctcgtcg gttatttttg acgccatgca atcgtcggcg accggcaagt
     1321 acgatgtcgt actgattgac acggccgggc gtctgcatac taaaattaat ctgatggaag
     1381 aacttaaaaa aatgcgtcgc gtgatcgaaa agcatattcc gggagcgccg catgagacga
     1441 ttttggtgct tgacggcaca acgggccaaa atgctattca acaggcgcga cagttttcgc
     1501 aagccgtgca ggtctccgga ttgattctga ccaaactgga tggtacggcc aaaggcggcg
     1561 tggtactggc gattcagcag gaattgaaaa tccctgtact gtatatcggt gtgggcgaag
     1621 gcattgatga tcttgaagcg tttgacccgc atgcattcat tcaagccatc gtggaataat
     1681 ttgttgagcg ttcatttata cgtgtgtcaa actgtcgtcg aatgaaaaaa atacgcctca
     1741 tactgggcat tcacaatcat cagcctgttg gcaattttga ttttgtattg gataaggctt
     1801 ataaaaatgc ctatcggcct tttcttgata tgctgcggga ttttcccggc gttcgcgtag
     1861 gcatgcatac gtcgggtatt ttgtacgaat ggcttgataa acattatcct gaatacagca
     1921 tacagctgcg cactttgacg gatcgcggac agatcgaact tatcggcggc ggattttatg
     1981 agcccatact tccgattatc ccgcatcgtg atcaggtcgg gcaaattcaa cgcttacaaa
     2041 aatatctttt gcaacggtac gaacaagccg cgcaaggtat gtggctggcc gaacgtgtgt
     2101 gggaaccgca tttaccgtcg gtgctcagtc aagtcggagt gcggtatgtg atactggatg
     2161 atacgcattt ccttcacgcc ggcttgtcgg tcgatgattt gagcggatac tacatcacgg
     2221 aagaccag
//
LOCUS       DAOQNQ010000430         2219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_30970_length_2219_cov_4.162662, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000430 DAOQNQ010000000
VERSION     DAOQNQ010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30970_length_2219_cov_4.162662"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PK754_15935"
     CDS             <1..349
                     /locus_tag="PK754_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11043.1"
                     /translation="EMFPNLRSYAKFLRRKDTHGFDPDEVSKENKFSSTSYRSINIVM
                     DIPIRVDDFLYTPDFSEFGRIIFVPVEIQIMDQMTYQNNEIGEGSHSKYKQRQMDAVN
                     QRLGLQQNGNSPG"
     gene            346..1053
                     /locus_tag="PK754_15940"
     CDS             346..1053
                     /locus_tag="PK754_15940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNB11044.1"
                     /translation="MISVIIPVLNEGQWITYCIEQLKHQNGAFEVIVSDGGSTDGTLE
                     RVSELKNLSFPCTVVSGDKGRANQLNRGAQAAKGEVLLFLHVDCELPPNAFKSVEQFL
                     SDPQCIGGGFQKKYNPENILMRLYRIMINTIRTLWLRNLVGTNAIFVRSRVFHQLGGF
                     KQVSLLEDVIFCDAMKKAGRMVIIKDPVIVSSRRYFSSGIVKRIGIAAEVIIRFRVLG
                     ASPEELEMRYHRRMKKR"
     gene            1054..1716
                     /locus_tag="PK754_15945"
     CDS             1054..1716
                     /locus_tag="PK754_15945"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNB11045.1"
                     /translation="MPNLRLQEFSAVLFDFDGVLMDSIGDHHRSWNSVFSEYGVSISW
                     DEFCLLEGQSLFVIAEQLCMNHGVDIKHGQEIGKKKNNLYLSTAKPKLYDGAEDLIEW
                     FRKQGLKTGLVTGAHRDRFYRTVGPDFTKRFEVIITADDVRKTKPDPEPYTTAATRLS
                     VSPERCIVIENAPLGVTAAKKAKMVCIALTTTLDQKYLNEADIVIPDLFSLKAMAHAA
                     FS"
     gene            1700..>2219
                     /locus_tag="PK754_15950"
     CDS             1700..>2219
                     /locus_tag="PK754_15950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB11046.1"
                     /translation="MQPFLEKKNVPPYSILADFYDGMMEHVDYRTWSSFIHQIFERYG
                     SGISTVCEFACGTGNFAAAMAAFPYTMICSDRSETMIMTARKKPVAQSGKILFFTGDM
                     LSFKPSVECEAALCLYDSVNYLMKAEDILNFFNHVSECTTGRGIFVFDVCTEKNSLDH
                     FDQRYELDRTHHY"
BASE COUNT          630 a          455 c          513 g          621 t
ORIGIN      
        1 tgagatgttt cccaatctgc gatcgtacgc aaaattcctc agacgaaaag atacgcatgg
       61 tttcgatccg gatgaagtat ccaaagaaaa taaattttca agcacaagct atcgttccat
      121 caatatcgtt atggatatac cgatacgtgt ggatgatttt ttatacacac cggatttttc
      181 cgaatttggc cgtattattt ttgtgccggt cgaaatacag atcatggacc agatgacgta
      241 tcagaataat gagatcggtg agggcagcca tagtaaatac aaacagcgac aaatggatgc
      301 tgtcaatcaa cgccttggtc tccaacaaaa cggaaactct ccgggatgat ctctgtcatc
      361 atacctgtcc tcaatgaagg ccagtggata acctactgta ttgaacaact gaagcatcag
      421 aacggcgcat tcgaagttat cgtttccgac ggtggcagta cagatggaac gctcgaacgt
      481 gttagtgaat tgaaaaattt atcttttccg tgcaccgttg tatcgggtga taaagggcga
      541 gcgaaccaac ttaatcgggg agcccaagcc gctaaaggtg aggtgctgct ctttttgcat
      601 gtagattgcg aacttcctcc gaatgcgttt aaatctgttg aacaatttct gagcgatccc
      661 caatgcatcg gcggcggatt tcaaaaaaaa tataatcccg aaaatatact catgcggttg
      721 taccgtatca tgatcaatac tatccgtaca ctttggttgc gtaatctcgt tggcaccaat
      781 gcgatctttg tccgatcacg cgtatttcat cagcttggcg gatttaaaca agtgtcctta
      841 ttggaagatg tgatattttg tgatgcgatg aaaaaagccg ggcgaatggt catcataaaa
      901 gatccggtta tcgtttcgtc gcgccgatat ttttcatccg gcatcgtcaa acgcattggt
      961 atcgctgcgg aagtgatcat tcgctttcgt gtgctgggcg cttcaccgga agaattagaa
     1021 atgcgttatc atcgcagaat gaaaaaacga tagatgccaa acttacgttt acaggaattt
     1081 tcagccgtac tctttgattt tgacggagtg cttatggata gcatcggcga tcaccatcgc
     1141 tcttggaaca gtgttttttc tgaatacggc gtttccataa gctgggacga attttgttta
     1201 ctcgaaggcc aaagcctttt tgtaatagcc gaacaattat gtatgaacca cggcgtggat
     1261 attaaacacg gtcaggagat tggaaaaaag aagaataatt tatacctgag tacggcaaaa
     1321 cctaagcttt acgatggggc tgaggatttg atcgaatggt ttcgcaaaca aggtctgaaa
     1381 accggattgg ttaccggagc gcatcgggac cgtttttata gaacagtggg gccggatttt
     1441 acaaaacgat ttgaagtcat cataacggct gatgacgtta ggaaaaccaa acctgatccg
     1501 gagccctaca cgacagcggc gacgagactt tcggtatcac cggaacggtg cattgtgata
     1561 gaaaacgcac ctttgggcgt aacagcagcc aaaaaagcga agatggtgtg tatcgcactg
     1621 acgacgaccc tagatcaaaa atatttgaat gaagcggata ttgtaatccc ggatttattt
     1681 tctctgaaag cgatggcgca tgcagccttt tcttgaaaaa aagaatgtac ctccgtattc
     1741 tatacttgca gatttttatg acggtatgat ggagcatgtg gattaccgca cgtggtcgtc
     1801 attcattcat cagatcttcg aacgctacgg gtccggtata tcaaccgttt gtgaatttgc
     1861 ctgtggaacc ggaaattttg ccgcagcgat ggccgcgttt ccttatacga tgatatgcag
     1921 cgatcgctct gaaaccatga tcatgacggc acgtaaaaaa cccgttgctc aatcaggtaa
     1981 gatacttttt tttacgggcg atatgctttc tttcaaacct tctgtcgaat gcgaagcagc
     2041 gctttgccta tatgatagtg tcaattatct gatgaaagcg gaagatattc tgaatttttt
     2101 taatcatgtt tccgaatgta caaccggccg tggtattttt gtttttgatg tctgtactga
     2161 aaaaaattca ctggatcact tcgatcagcg gtatgaactg gatcgaacac atcattaca
//
LOCUS       DAOQNQ010000431         2215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_31086_length_2215_cov_4.846759, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000431 DAOQNQ010000000
VERSION     DAOQNQ010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31086_length_2215_cov_4.846759"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /gene="secG"
                     /locus_tag="PK754_15955"
     CDS             complement(<1..137)
                     /gene="secG"
                     /locus_tag="PK754_15955"
                     /inference="COORDINATES: protein motif:HMM:NF015779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNB11047.1"
                     /translation="MFAFLVVLHIIVSILLIGAILMQSSKGGGLAGLGGGASTILSGR
                     QA"
     gene            complement(174..935)
                     /gene="tpiA"
                     /locus_tag="PK754_15960"
     CDS             complement(174..935)
                     /gene="tpiA"
                     /locus_tag="PK754_15960"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNB11048.1"
                     /translation="MRKKVIVGNWKMHKGPNAALHLAKSLKLKLTDIRKTEMAVCPPS
                     ISIAAVRDVLKDTKIKVGAQNMYLHKYGAYTGEISAEMIKESGCEYVIIGHSERRQHF
                     FETDTTVNHKIEFALQELLTPIVCVGETLEQRQEGHTESVIRQQYLGAMKNLDSDKAA
                     KIIIAYEPIWAIGTGKNATVAEAEAVHKFIRDLARESFGKTISDKMIIQYGGSVKPDN
                     AAALLGCVDIDGALVGGASLEAESFAAIIHAAEKI"
     gene            complement(928..2124)
                     /locus_tag="PK754_15965"
     CDS             complement(928..2124)
                     /locus_tag="PK754_15965"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNB11049.1"
                     /translation="MNKLTIDQVHVAGKKVLVRVDFNVPLDDTLRITDDTRIRESLPT
                     IKKILSDGGIPVLMSHLGRPKGKKNPAMSLKPCAVRLSELLGQPVIMAEDCIGDTVEK
                     QAAGLSKGQVLLLENLRYYNEEEANDIAFAEKLAKLGDIYVNDAFGTAHRAHASTEGV
                     TRYFKQNIAGYLMEKELRYLGNAVQNPKRPFVAIIGGAKISGKIDVIENLMKKVDTII
                     IGGGMAFTFAKVMGYEIGNSLFEAEKADLAKSIIERAKSGTTRVLFPVDAVVATEFKN
                     EAEIKTVSMDQIPSGWMGLDIGPVTIDMFRNEINSAGTVVWNGPMGVFEMPNFAKGTK
                     AIAESLVEATQKGGVTIVGGGDSAAAITELGLEKQVTHVSTGGGASLEFLEGKILPGV
                     EALTHA"
BASE COUNT          570 a          562 c          444 g          639 t
ORIGIN      
        1 gcttgacgtc cgctaagaat agttgaagca ccaccgccga gacctgcaag gccgccgcct
       61 ttgctggatt gcataagaat cgcaccgatc agcaaaatgc tgacaatgat gtgtaaaaca
      121 acaagaaacg caaacatgga tttactcctg agtaatgatg aattataatt gaattatatt
      181 ttttcggctg catgtatgat cgcggcaaac gattcggctt cgaggctggc gccgcctacg
      241 agagcgccgt caatatccac acaacctaat aatgcggcag cattatcggg cttcacgctc
      301 ccgccatatt gaatgatcat cttatctgat attgtttttc caaatgactc ccgcgctaag
      361 tcgcgtataa acttatgtac cgcttcggct tcggcaaccg tagcattttt gccagttcca
      421 atagcccaaa tcggttcgta tgcaataatt attttggcgg ctttatctga atccagattt
      481 ttcattgcac cgaggtattg ctgacgaata actgattcgg tatgtccttc ctgacgctgt
      541 tcaagcgtct caccgacgca tacaatcggc gtcagtaatt cctgcaaagc aaattcaatt
      601 ttatgattca ccgtcgtatc ggtttcaaaa aaatgttgcc gtctttccga atgcccgatg
      661 atcacatatt cgcagccgga ttctttgatc atttcggcag aaatctcacc ggtataagct
      721 ccgtatttat gcaaatacat attctgcgca ccgactttga ttttagtgtc tttcaaaaca
      781 tcacgcaccg cagcaatact gatggaaggg gggcaaacag ccatctccgt tttacgaata
      841 tcggttaatt tcagtttcag cgatttggct aagtgcaggg ccgcattcgg gcctttatgc
      901 attttccaat ttccgacgat taccttttta cgcatgggtc aaagcttcca caccgggcaa
      961 aatttttcct tcgagaaatt cgagagacgc accgccgccc gtggacacgt gcgtgacttg
     1021 tttttcaaga cccaattccg ttatcgcggc cgccgaatca ccaccgccga caatggttac
     1081 cccgcctttt tgtgtcgctt cgaccaaact ttcggctatg gctttagtgc cttttgcaaa
     1141 attgggcatt tcaaaaacac ccatcggccc attccacacg accgttcctg cggaattgat
     1201 ctcgttacga aacatatcaa tcgtaaccgg accgatgtct aaacccatcc agccggacgg
     1261 tatttgatcc atcgaaacgg ttttaatttc ggcttcattt ttaaactccg tagcgacaac
     1321 agcatcaacc ggaaacaaaa cgcgtgtagt accgcttttc gctctttcga ttatagactt
     1381 tgctaaatca gctttttcgg cctcaaaaag cgaattacct atttcatacc ccatcacttt
     1441 ggcaaaagta aacgccatac cgccgccgat aatgatggta tccacttttt tcattagatt
     1501 ttcgattaca tctattttac cggaaatttt ggcgccgccg atgatggcaa caaaaggccg
     1561 tttggggttt tgaaccgcat tgccgagata acgcaattct ttttccataa ggtaacccgc
     1621 gatattttgt ttgaaatacc gcgtaacgcc ctcggtagat gcatgtgcgc ggtgtgctgt
     1681 gccaaaagca tcattgacat atatgtcgcc aagcttggcc agtttttcag caaaagcgat
     1741 atcgttggct tcttcttcat tataatatcg gagattttca agcaacaata cttgcccttt
     1801 ggataaaccc gccgcttgtt tttcgacggt atcgccgata caatcttccg ccattattac
     1861 gggttgtccc aaaagttctg aaagcctcac agcgcaaggt tttagagaca tcgcaggatt
     1921 ttttttccct tttggccgcc ccaaatgact catcaaaacc ggaataccgc cgtcgctcaa
     1981 tatcttcttt attgtcggca gagattcacg aatgcgtgta tcgtccgtaa tgcggagtgt
     2041 gtcgtccaac ggaacattaa aatcaacccg tacaagtacc ttttttccgg cgacatggac
     2101 ttgatctatc gtaagcttat tcatcatcgt ttcgtccgtt taggctttgg ctattttcat
     2161 caaaagatca acgcaacgat tggaataacc ccattcgtta tcgtaccaag aaacg
//
LOCUS       DAOQNQ010000432         2208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_31263_length_2208_cov_5.297724, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000432 DAOQNQ010000000
VERSION     DAOQNQ010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31263_length_2208_cov_5.297724"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PK754_15970"
     CDS             <1..518
                     /locus_tag="PK754_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HNB11050.1"
                     /translation="LWPCPEGKETKWRYHAAYDPAAKGDAWDFYGKPEGKAWIWIRPY
                     EPPPEMPDDDYPYWLNTGRVLEHWHSGSMTRRIPVLHQAVPSSYVELNPDDAKELGIR
                     HGDKVRLTSRRGSIVMSASINERGVPPHGMVFVPFFDESHLINELTLDAFCPISRQPD
                     FKKCAVKVERA"
     gene            515..1213
                     /locus_tag="PK754_15975"
     CDS             515..1213
                     /locus_tag="PK754_15975"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HNB11051.1"
                     /translation="MNLKKLLLPENRVIFISLSALLMLCVIVIVGQSVIQSGAEAYTP
                     SVKTPGEAVLPDEAGVFKQSDQALYYLETLHQQGSRTLAAFYERRAFDGAPPVIPHEV
                     TDERSVGGNTCLQCHENGGFVPKFNAYAPVSPHPQFLSCRQCHVPVTTRNVFRASGFT
                     PVKPPAINRSALTGAPPQIPHDLQMRENCLACHGGPASVREIRTPHPDRVNCRQCHGA
                     IEDNTAWTRPAPTP"
     gene            1233..1886
                     /locus_tag="PK754_15980"
     CDS             1233..1886
                     /locus_tag="PK754_15980"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNB11052.1"
                     /translation="MRQHMLFSANTRNFKILRTMAGVCLAVFFVSCGHDHAQGLLEKL
                     DTAPAWSDSAYTHVAPTADTASLHAVQLVERPLYVVPRTPLIKKYPCTECHTKPLGQM
                     KSSDPSGRKAHWDKNLQHATTNVLQCTVCHDERNLNNLRSITGAVIAFNDSYQLCQQC
                     HSRQFNDWQGGAHGKRVGGWAPPRTVALCVECHNPHQPRWDIRWPAQPSLTMQRLIK"
     gene            1905..>2208
                     /locus_tag="PK754_15985"
     CDS             1905..>2208
                     /locus_tag="PK754_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11053.1"
                     /translation="MSHCQCQHTPDPRREGLRDLLAENVLSDWLKRDTEWDYLRATPS
                     LENVMRWSQYRDDHTSVRPDPKATAADHVLEQSMDRRSALQNMAAAFVAGLGLLQTA"
BASE COUNT          524 a          612 c          556 g          516 t
ORIGIN      
        1 ttctttggcc atgtcccgag ggcaaagaaa cgaagtggcg ttaccatgcc gcctacgatc
       61 ctgcagccaa aggcgatgcg tgggattttt acggcaaacc tgaaggcaaa gcttggatat
      121 ggattcgacc gtacgaaccg ccgccggaaa tgccggacga tgattacccg tactggctca
      181 atacaggacg cgttttggaa cattggcact ccggttcgat gactcgccgc atccctgtat
      241 tgcatcaggc cgttccttct tcctacgtgg aactcaatcc cgacgatgcc aaagaacttg
      301 gtattcgtca tggcgacaaa gtgcgcctga catcgcgtcg tggcagcatc gttatgagtg
      361 cttcgatcaa tgagcgcggt gtccctccgc acggtatggt gttcgttccg ttttttgacg
      421 agtcccacct gatcaacgaa ttaaccctgg atgctttttg tcctatttcg cggcagccgg
      481 atttcaaaaa atgcgccgta aaggtggaaa gagcatgaac ctcaagaagc ttttgctccc
      541 cgaaaaccgc gtgatcttta tctcactgag cgcgcttctg atgctgtgcg tcatcgtgat
      601 cgtgggacaa agcgtcatcc agtccggagc ggaagcctac acaccttcgg taaaaacacc
      661 gggcgaagcg gtgcttccgg atgaagccgg tgtttttaaa caaagcgatc aagctcttta
      721 ttatcttgag actctccatc aacagggatc acgcacgctt gctgcgtttt atgagcgccg
      781 ggcgtttgac ggtgcaccgc cggtaatacc gcacgaagtg acagatgaac gcagtgtcgg
      841 tggtaacacc tgcttgcaat gccatgaaaa cggcggtttt gtgcccaagt ttaacgcgta
      901 tgcaccggta tcgccgcacc ctcagttttt gagctgccgt cagtgtcacg taccggttac
      961 gacgcgcaac gtatttcgcg catcgggatt tacgccggtg aaaccgccgg ctatcaatcg
     1021 ttccgctctg accggtgcac cgccgcagat accgcatgac cttcagatgc gggaaaattg
     1081 tttggcgtgc cacggcggac cggcatccgt tcgcgaaata cgcacaccgc atccggatcg
     1141 tgtcaactgc cgccaatgtc atggagctat cgaagataat accgcctgga cccgtccggc
     1201 gccaactccg taatcgctta acaaaaaaat ttatgcgaca acacatgttg ttttctgcga
     1261 atacgcgcaa ttttaaaatt ctacgcacta tggccggtgt ctgtctggct gtattttttg
     1321 tttcttgcgg acacgatcac gcacaaggcc ttttggaaaa attagatacc gctcccgcat
     1381 ggtcggattc cgcttatacg catgtcgctc cgaccgcaga tactgcgtct ttgcatgccg
     1441 tgcagcttgt agagcgacct ctctacgtcg taccccgcac accgctaatc aaaaaatatc
     1501 cctgtacgga atgccatacc aaacctttgg gacagatgaa atctagcgat ccctcgggcc
     1561 gaaaagcgca ctgggataaa aacctacagc atgcgacgac caacgttctg caatgcactg
     1621 tttgtcacga tgaacgaaat ttgaataacc tgcgcagcat caccggtgca gtcatcgctt
     1681 ttaacgatag ttatcagttg tgccaacaat gtcattcgcg tcaattcaac gattggcagg
     1741 gcggtgcaca cggcaaacgt gtcggcggtt gggcgcctcc gcgcaccgta gcgttatgtg
     1801 tcgaatgcca caatccgcat cagccgcgtt gggatatacg ttggccggcg cagcccagtc
     1861 tgacgatgca acgattgatc aaataatttt ataacggata cagcatgtca cattgccaat
     1921 gccaacatac gccggatccg cgccgagaag gtctgcgtga tttacttgca gaaaatgttc
     1981 tctccgactg gctcaaacga gacaccgaat gggactatct gcgtgcgacg ccgtcgctcg
     2041 aaaatgtcat gcgctggagt cagtaccgcg atgaccatac gtcggtacga cctgatccca
     2101 aagcaacagc cgctgatcac gtgttagaac aatccatgga tcgccgttct gccttacaaa
     2161 atatggccgc ggcattcgtc gccggcttag gattactgca aaccgcct
//
LOCUS       DAOQNQ010000433         2207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_31291_length_2207_cov_4.935874, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000433 DAOQNQ010000000
VERSION     DAOQNQ010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31291_length_2207_cov_4.935874"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PK754_15990"
     CDS             complement(<1..841)
                     /locus_tag="PK754_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11054.1"
                     /translation="MLPYISRMMIRIYKILLLLCVSSWIAAQSPDELIRRGQERIYTL
                     DFKSADAVFDSLIALDPLHPRGYFFKGSSYFYQILSGHNTRMTGDEFEKWNDKTVEVA
                     EQYAAQAHQETEGDFYRGAAYGNSARYHAVHGEFVKAFYYAKRSKSLHEDVIEADPLY
                     YDAYLSIGMYNYYAAAAPRWMDAIGSLFGLSGDRATGIAQLEKTYRHGKIFNLEAQFF
                     LANVYLEEGDYETSLRIFSQLMDRFRSNPYLINQAGMVSFYLENYSVAEQIFQQSILQ
                     TSQT"
     gene            complement(873..1964)
                     /locus_tag="PK754_15995"
     CDS             complement(873..1964)
                     /locus_tag="PK754_15995"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB11055.1"
                     /translation="MKRLSRRRFLRHCAVQSVALTAGQSAMGMLPEEKTLVEYVRNEA
                     LITVRENYAGNPMRDGIFLNSDLNPNLKPFSVIFKWFLSENPQREEKKNDPFRVTTHN
                     VKDFRAINENGLMWLGHSSFLIRLGNKWLLTDPCLTAAPLQKRYSELPMPISHLESVD
                     YVLLSHNHYDHLDSDTLSTWRSRAMKILLPLRLGSTVRSINKYVTIQEAGWFQKYDLE
                     EDFEIILLPAIHWSKRGLWDNNRSLWGSFLIRTPRTTIYFSGDTAYGEHFGEIARLFG
                     KIDIALMPIGAYKPQHIMKSAHTSPQEAVQGFHDLKAKTFIPMHYGTFDLADEPMGEP
                     VKLLRNMKADGLINGELQIPDAGAWITFD"
     gene            complement(1961..>2207)
                     /locus_tag="PK754_16000"
     CDS             complement(1961..>2207)
                     /locus_tag="PK754_16000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11056.1"
                     /translation="FSFTARQHIGSFVYAFLFFIVFTIADYVFLNKLFFEFLNRTGQW
                     RGGNTMMGVFALLLCVMAGGLVLANYLIRYTIKKYKS"
BASE COUNT          662 a          528 c          454 g          563 t
ORIGIN      
        1 tagtttgcga tgtctgaagg atggattgct gaaaaatttg ctctgccacg gagtagtttt
       61 ccaaataaaa actgaccatg cccgcttgat tgatcagata cgggttactg cgaaagcgat
      121 ccattaactg ggaaaaaatt ctcaatgacg tttcgtaatc accttcttcg agatacacat
      181 tagcgagaaa aaattgtgct tcaagattaa atatttttcc gtgccggtaa gttttttcca
      241 actgcgcaat acccgtcgcc cgatctccgc tcaatccaaa tagcgaaccg atggcatcca
      301 tccagcgcgg tgccgccgcc gcataataat tatacatacc gatactaagg tatgcgtcat
      361 aatacaaagg atccgcttcg atcacatctt catgcaaact tttagagcgt ttggcataat
      421 aaaacgcttt gacaaattcg ccatgaacgg cgtgatatcg tgccgaattc ccatacgcgg
      481 cgccgcgata aaaatcacct tcggtttcct gatgggcttg tgcggcatat tgttcggcca
      541 cttcgaccgt tttatcattc catttttcaa attcatcccc tgtcatacgc gtgttatgac
      601 cggataaaat ctgataaaaa tacgaagacc ctttaaaaaa ataaccacgc gggtgcaaag
      661 gatcgagtgc gatcagcgaa tcaaacacgg cgtcggccga tttgaaatcc agcgtataaa
      721 tgcgttcctg accgcgacga atcaattcgt ccggcgattg tgcggcgatc catgagctga
      781 cacataagag cagcaggatt ttataaatgc ggatcatcat gcgggaaata taaggaagca
      841 cacactaaac acaaaggcta ttcagggcat gctcaatcaa aagttatcca agcaccggca
      901 tccggtattt gcagttcacc gtttattaat ccgtcggctt tcatgtttcg aagtaatttg
      961 accggctcgc ccatcggttc gtcagcaaga tcaaatgtac cgtaatgcat cggaataaaa
     1021 gtttttgctt tcagatcatg aaatccttgt accgcttctt gcggactggt atgggcagac
     1081 ttcatgatat gctgcggttt atacgcgccg atcggcatca aagctatgtc aattttacca
     1141 aataaccggg cgatttcgcc gaaatgctct ccataggccg tatcacccga aaaataaatc
     1201 gtggttcgcg gcgtacgaat caaaaaactt ccccacagtg agcgattatt atcccacaat
     1261 ccgcgtttag accaatgaat agccgggagt aaaataattt caaaatcttc ttccagatca
     1321 tatttttgga accaaccggc ttcctgaatg gtcacatatt tattaatgga tcgaaccgtc
     1381 gaaccgagcc gcaagggcag cagtattttc atcgcacgtg agcgccaagt gctcagcgta
     1441 tccgagtcaa gatgatcata gtgattgtgc gacaataaaa cataatccac ggactccaaa
     1501 tgtgaaatcg gcatcggcag ctcggaataa cgtttctgca acggcgccgc cgtaaggcac
     1561 ggatcggtaa gtaaccattt attacccagg cggatcagaa aagaagaatg accaagccac
     1621 atcaaaccgt tctcattaat tgcgcgaaaa tctttcacgt tatgtgttgt tacacgaaac
     1681 ggatcatttt tcttttcttc gcgctgcgga ttttccgata aaaaccattt aaaaatcaca
     1741 ctgaacggtt tgagattggg attcaaatcg gaattcagaa aaatgccatc ccgcatcgga
     1801 tttccggcat aattttcacg aacggtgata agcgcttcgt tgcgcacgta ttcgaccaaa
     1861 gttttttcct ccggcagcat acccatcgcg ctctggcctg cagtcagcgc caccgactgt
     1921 acagcacaat gtcggagaaa ccgtcgtcgg gatagccttt tcatgattta tattttttta
     1981 tagtgtaccg tatcagataa ttagccaaaa caagcccgcc ggccattacg cacaacaaca
     2041 atgcaaaaac ccccatcatg gtatttccac cgcgccattg acctgtacga ttcaaaaatt
     2101 caaaaaaaag tttatttaaa aaaacataat cggcgatcgt aaaaacgata aaaaacaaaa
     2161 acgcatacac aaacgatccg atgtgttgac gggcagtgaa tgaaaaa
//
LOCUS       DAOQNQ010000434         2203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_31393_length_2203_cov_4.874302, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000434 DAOQNQ010000000
VERSION     DAOQNQ010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31393_length_2203_cov_4.874302"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..2147
                     /gene="ligA"
                     /locus_tag="PK754_16005"
     CDS             132..2147
                     /gene="ligA"
                     /locus_tag="PK754_16005"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNB11057.1"
                     /translation="MHDHIQKKVHKLRDEINRHDYLYYVLGQPAITDEAYDKLMRELS
                     EIETRHPELVTPDSPTQRVGSDLTKHFPTVRHHQPMMSLGNTYSREELSDFDRRVREG
                     LEGASFEYVCELKFDGVAMSLIYEYGHFVRGVTRGDGEKGEDVSINLKTIRSIPLRLD
                     AANAHLEHIEVRGEVLMLREDFIKVNARRTEAGEPPFANPRNLSAGTLKLQDPRLVAA
                     RPLKFFSYYLRELGAEKKLNSHSESLTALESLHFPVMKTYRVCKTLDEVFAFCEEWEA
                     KRDTLPFEIDGAVIKVNAFRQQETLGATAKSPRWAIAYKFKAQKVETRVRDIVLQVGR
                     TGTVSPVAELEPVFLAGSTISRVTLHNEDFLQEKDIRIGDTVRIEKGGDVIPKITDVV
                     FDKRPAEAHVFRFPKACPVCGEPVVKTEGEAAWRCENITCDAQIKKRIEHFCSRDAMD
                     IENCGEAVVAQLVDAGCIHDFADLYYLQKEALLALERWGEKSATNLISAIEHSKHNSL
                     ERLIYGIGIRFVGEESAKYLAKHFKSLDALRQADGETLTAIEGIGERTALSIKQFFDS
                     AQNRKVLDKLTQAGVQTMYISSAVELPPIFSGKTFVLTGTLPSLGRNDAKKMIEDRGG
                     KISGSVSKKTDYVLAGAEAGSKLDKAKELNVPVIDETEFLKMISESQ"
BASE COUNT          597 a          475 c          582 g          549 t
ORIGIN      
        1 gtttaagaag tctgtatctg tcgccgtcct atatttataa aacagcacga tcagtcaatt
       61 cgccgaaggt attattgaat tatgcgcgct acggtgtacg tctttttaga aatttactga
      121 aggcctgacg catgcacgat catattcaaa aaaaggttca caaattgcgt gatgaaatca
      181 atcgtcacga ttatttgtat tacgtgcttg ggcagccggc gatcacggat gaagcgtacg
      241 ataaactgat gcgcgaatta agtgaaattg aaacgcggca tccggaatta gtgacccctg
      301 attcgccgac gcaacgcgtg ggttcggatt tgaccaaaca ttttccgacg gtgcgtcatc
      361 accaaccgat gatgagcctc ggtaatacct acagccgtga ggaactttcc gattttgacc
      421 ggcgcgtgcg ggaggggctt gaaggggcat catttgagta tgtttgcgaa ctcaaatttg
      481 acggcgttgc tatgagcctg atttacgaat acggtcactt cgtacgaggg gttacgcgcg
      541 gggacggaga aaaaggcgag gacgtcagta tcaatctcaa aacgattcgt tcgatcccgc
      601 tgcgattgga tgcggccaat gcccatctgg agcatatcga agtgcgcggg gaagtattga
      661 tgttgcgcga agatttcatc aaagtcaacg cacggcgtac ggaagcaggt gaaccgccgt
      721 ttgcgaatcc gcgcaatctg tcggcaggaa cactcaaatt gcaagatccg cgtttggtag
      781 cggcccgccc tttgaagttt ttttcatatt acctgcgcga acttggcgct gaaaaaaaat
      841 tgaattcgca ttcggaaagt ctgacggcgt tggaatcgct ccattttccc gtaatgaaaa
      901 cgtatcgcgt gtgcaaaaca ttagatgaag tatttgcgtt ttgcgaggaa tgggaagcca
      961 aacgcgacac cttacctttt gaaattgacg gcgcggtgat caaggttaat gcctttcgtc
     1021 agcaggaaac gctgggtgcg acggccaaat ccccgcgttg ggccattgcg tataaattta
     1081 aagcacaaaa agtcgaaacc cgcgtacgtg atattgtatt gcaagtcgga cgcaccggta
     1141 cggttagccc agttgcggaa ttggagcccg tgtttttggc cggttccacg atcagccgtg
     1201 tgactttgca caacgaagat tttttgcagg aaaaagatat tcgtatcggc gataccgtgc
     1261 gtatcgaaaa aggcggcgat gtgattccca aaatcaccga cgtggttttt gataaacgcc
     1321 ctgcggaagc gcacgtattt cgttttccca aagcgtgtcc ggtttgtggt gaacctgtcg
     1381 tcaaaacaga aggcgaggcg gcgtggcgct gtgaaaacat cacctgcgat gcacagatca
     1441 aaaagcgtat cgaacatttt tgttcgcgcg atgcgatgga tattgaaaat tgcggtgaag
     1501 ccgttgttgc gcaattggtc gatgccggat gtattcatga ttttgcggat ttgtattatc
     1561 ttcaaaaaga ggcgttgctt gcgttggaac gttggggtga aaaaagcgct accaatctga
     1621 tctcggctat tgaacacagt aaacataatt cactcgagcg tttgatctac ggcataggca
     1681 ttcgttttgt cggcgaagaa agcgccaagt atttagccaa acattttaaa tccctcgacg
     1741 cactgcgcca agcggatggt gagacactca cggccatcga aggtatcgga gaacgcacgg
     1801 cgctgagtat taaacagttt tttgacagcg cacaaaaccg caaagtgctg gacaagctga
     1861 cgcaagcggg cgtacagacg atgtatattt catctgccgt agaattgcct ccgatattta
     1921 gcggaaaaac atttgtactt acggggacgt tgccttcact tggccgcaat gacgctaaaa
     1981 aaatgatcga agatcgcggt ggaaaaatat cgggttccgt cagtaaaaaa acggattatg
     2041 ttttggccgg tgcggaggcg ggttccaaat tagataaagc caaggaactg aatgttcctg
     2101 tgatagacga gacagaattc cttaaaatga tcagcgaatc ccaatgaaaa gaacattgac
     2161 attaaaatgg ttacccgttc tgttaattgg gttgatcacg gcc
//
LOCUS       DAOQNQ010000435         2192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_31707_length_2192_cov_5.358914, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000435 DAOQNQ010000000
VERSION     DAOQNQ010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31707_length_2192_cov_5.358914"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2192)
                     /locus_tag="PK754_16010"
     CDS             complement(<1..>2192)
                     /locus_tag="PK754_16010"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNB11058.1"
                     /translation="SDSKFYVSYDYGLSWIPRGILNAGGDIRNITVNPLDTNVVIAVG
                     FGIFKSNDGGKTWREALANVTIDGESIDYNYLHPDTMYFVDFSMRKFYLSADTGSSWV
                     ERSTVGFGPICTVVSHPQNPNIIIIGSGDTRIERSTDMGLTWQLVKQGNPYFSEIPKM
                     AWDKSDPNFIYGSTYVDNQYSFFRSTNGGLTWEDAGIYGMYTWGLDYGIDRGDVFIGT
                     FGYDGADRGIYRSVDRGQSFMKVGTANLGDTFLNTWMIKTAADGNVFFVNENKVYRTQ
                     FGVEGKVTGVVRTQNNTPLRFASIFVDETGDSLFIGNINGNYTLALPPGQYTLRARVA
                     GVESTPVQVTITADNTITQDFQVPISETFHTVSGTVTAIDGEPINVNIQLHGRYGNGS
                     RFTKETSTTSAFSFTNLSSLSLYDSIVVIPQELPYITSIIKPLTLDASVPVSIDIADV
                     LIVSETYVPSGAYLEYYTNRLNEAGYKATGLEGYSLSSIPEHALLKTKYKTALWLTRR
                     IEMDMDPLWHDTLVSFIQKGGHVMIAGQDILEKNSSLPVFSEYLKLGFGGNYTTDPTI
                     QGIDGNLIGNGLNFSISQSTLPSRDRISFSNANASVAFRYGTLPEDADKYAGVYISNT
                     GFGGKAVVLGFDPYRVGDLALRTIFDRTLKYFNGEEGAFDLTVSVFQNSLIRNRGSIV
                     IAATHILQIPPIVKIAKGTDTTQVTVTALSGAKVYSGAFNLSEGSYKL"
BASE COUNT          617 a          505 c          473 g          597 t
ORIGIN      
        1 atgagtttat aactaccttc ggatagatta aaagcgccgc tatatacttt agcaccgctc
       61 aaagcggtca ccgtgacttg tgtcgtatcc gtacctttgg cgattttgac gatcggtggt
      121 atttgtaaaa tatgtgtggc tgctatgacg atactgccgc gattacggat cagcgaattt
      181 tggaatactg aaaccgtcaa atcaaacgcc ccctcttcac cattaaaata tttgagcgta
      241 cgatcaaata tcgttcgcaa tgccaagtcg ccgacacggt atggatcgaa ccccagtacg
      301 acggcttttc ccccaaaacc cgtattagaa atataaacgc ccgcatattt gtcggcatct
      361 tcgggcaagg taccgtagcg gaaagcgacg cttgcattgg cattggaaaa actgattcgg
      421 tcgcgtgaag gcaacgtgga ttgagaaatg gaaaaattca aaccattgcc tataaggtta
      481 ccatcaatgc cctgaatagt aggatcggtc gtgtagtttc ctccaaaacc taatttcaga
      541 tattcggaaa atacaggtaa cgaactattt ttctccaaaa tatcctgacc agcgatcatc
      601 acatgaccgc ctttttggat aaatgatacc agcgtatcgt gccatagcgg atccatatcc
      661 atttctatgc gccgtgtcag ccataacgcc gttttatatt ttgttttcaa aagggcgtgt
      721 tcggggatag aactcaacga atatccttcc aatcccgtcg ctttataacc cgcttcgttt
      781 aaccggttgg tataatattc gaggtaagca ccacttggga catacgtctc actaacgatc
      841 aggacatctg caatatctat gctaaccgga acggatgcat ccaaagttaa cggtttgatg
      901 attgaggtga tatacggcag ttcttgcggt attacaacaa tcgaatcata tagtgataat
      961 gatgacagat ttgtaaacga aaaagccgaa gtggttgaag tttctttggt aaaacgagac
     1021 ccattaccat aacgcccatg aagttggata ttgacgttga tgggttcacc gtcaatagct
     1081 gttactgtgc cactaacggt atgaaatgtt tcagaaatcg gtacttgaaa gtcttgagtg
     1141 atcgtattat cggctgtgat cgttacttgt accggcgtac tctctacacc tgcgacacgc
     1201 gctcgcagtg tgtattggcc cggaggcagc gctaatgtgt aattaccgtt tatatttccg
     1261 ataaacagcg aatctccggt ttcatccaca aaaatggaag caaaacgaag cggtgtatta
     1321 ttttgtgtgc gcacgactcc ggtgactttt ccttctacac caaattgggt tcgatatact
     1381 ttattctcat tgacaaaaaa aacattaccg tccgcggccg ttttgatcat ccatgtattt
     1441 aaaaaagtat caccgagatt agccgttccc actttcataa aactttgacc gcgatctaca
     1501 ctgcgataga tgccgcgatc cgcaccatca taaccgaatg tgccgataaa aacgtcaccg
     1561 cgatcaatac cgtagtcgag tccccaggtg tacattccgt aaatgccggc atcttcccaa
     1621 gtcaatccgc cgttggtact tcgaaaaaaa gaatactgat tgtccacata tgtacttccg
     1681 tagataaaat taggatcgct tttatcccat gccattttgg ggatttccga aaagtacgga
     1741 ttgccttgtt ttacgagttg ccaggtgagc cccatatcgg tggagcgttc gatgcgtgta
     1801 tcgccggaac cgataatgat aatattggga ttctgtggat gcgatacgac ggtacagatc
     1861 ggcccgaatc cgacagtaga acgttcaacc cacgacgaac cggtatcggc actgaggtag
     1921 aatttccgca tactgaaatc tacaaaatac atcgtatcgg gatggagata attataatca
     1981 atggattctc cgtcaatagt tacattggct aaggcttcac gccatgtctt acccccgtca
     2041 ttacttttaa aaataccaaa tccgacggct atgacgacat tggtgtccag cggattgacc
     2101 gtgatattgc gtatgtcgcc acccgcgttt aagatgccgc gaggaatcca cgacaagcca
     2161 tagtcgtacg atacataaaa tttgctgtca ga
//
LOCUS       DAOQNQ010000436         2186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_31896_length_2186_cov_5.439700, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000436 DAOQNQ010000000
VERSION     DAOQNQ010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31896_length_2186_cov_5.439700"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="PK754_16015"
     CDS             <1..1008
                     /locus_tag="PK754_16015"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB11059.1"
                     /translation="QGYKSIIGFSPYIEKEAMIRSSQITDGVIVKGINEQRFHSVLDL
                     KKDILKTHVSVSTPLGLRDYISNDSTSKKLPGIVIGKKLADKLAVGIGDKLTLFSLQN
                     SPSFLQQPVVRQYRVTAIYRSGLAEYDNVYVYVDLTEASKLFDYGASVSGYEIKLNDP
                     ESAKPTAEALNGELGFPYYAKTWYDNHRNLFGWLETNNFIMVIIFSLIIFVAAFNIVG
                     TLFMIVIEKTKEIGVLRSMGATRSEIRRIFLAEGLIIGFIGAVVGSILALALMLSQIY
                     FKWLSLDSDVYFMDSVPMEIRGEYFFVIASFSVLLCLLATLYPSRKAAFLDPVEAVRY
                     E"
     gene            1005..>2186
                     /locus_tag="PK754_16020"
     CDS             1005..>2186
                     /locus_tag="PK754_16020"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB11060.1"
                     /translation="MKKMPSEWFIALRYLKPRKDNFFVTLIGGISVLGVMVGVMSIIL
                     VLTVLNGFETEIKNRFIGFDAHGKIKMQNEIPMTSPDSVENILRKQYPINGAAPYILE
                     KAMITSRQSSHIVFVKGTDEKRLTQVSDLSKNMAAGKIDFEVQSEGLSGILLGLSVAI
                     NLDVSTGDTVTVISPSGVTGPFSAPPMRRYVVTGLFKTDMYEYDNAYVFISIPEAQKL
                     FEKDEAITGLDIRFENIAQASEYENAIRTEIGESFVYETWYDMHRDLYAAMKLEKWGS
                     LLLLSMIIMVAGFNIVSTLIMVVMQKTQEIGILKSIGANQKFISSIFVRQGLIVGSIG
                     IVAGCIVGYSICFIQLQWGIFKLPGDIFFLDAIPMELQWGDFFAVIVISVLLCLFATY
                     YP"
BASE COUNT          611 a          395 c          489 g          691 t
ORIGIN      
        1 cagggatata aatccatcat cggattttcc ccttatattg aaaaagaggc catgattcgt
       61 tcgtctcaaa ttacagacgg tgttattgtc aagggaatta acgaacaacg tttccattcg
      121 gtcttggatc taaaaaaaga tattctcaaa acacatgtat ccgtatcaac tccactgggc
      181 ttgcgtgatt atatttcaaa cgacagtacc tccaagaaat taccgggtat cgttattgga
      241 aaaaaattag ccgataaact tgcagtcgga ataggcgata aacttacttt gtttagcctc
      301 caaaattcac cgtcgttttt gcagcaacca gtagtccgtc agtatcgtgt aacggccatt
      361 taccgaagcg ggttagcgga atatgataat gtctatgttt acgtggatct tacggaagca
      421 tctaaattgt ttgattacgg tgcgtccgtg tcgggttatg aaataaaact aaatgatccg
      481 gaatcggcga aacccacggc cgaagctctt aatggtgaat taggcttccc gtattacgcg
      541 aaaacctggt atgataatca ccgtaactta tttggttggt tagaaactaa caatttcata
      601 atggtgataa tttttagtct tatcattttt gttgcggcgt ttaatattgt tggaacctta
      661 ttcatgattg tcatagagaa gactaaggag attggcgttt tacgatctat gggtgctacg
      721 cgttctgaaa tacgtcgcat ttttcttgcc gaagggctca ttattggttt tatcggcgcg
      781 gttgtcggtt cgatattggc tctcgcattg atgctgtcac agatatattt caaatggcta
      841 agtttggatt ccgatgtcta ttttatggat tctgttccaa tggaaataag aggcgaatat
      901 ttttttgtga ttgcttcatt ttccgtattg ttatgcctgt tggcgacgtt gtatccgagt
      961 cgtaaggccg cttttttaga tcctgtggag gccgtgcgat acgaatgaaa aaaatgccat
     1021 cggaatggtt tatagcttta cgttacctga aaccccgcaa agataacttt tttgtaacac
     1081 ttatcggcgg tatttcagta ttaggggtaa tggttggggt aatgtcaata atccttgtac
     1141 ttacggtgct caatgggttt gaaacggaaa ttaaaaaccg ttttatagga tttgatgcac
     1201 acggtaaaat aaaaatgcaa aatgaaattc ctatgacatc accggattcc gtagaaaaca
     1261 ttttgcgaaa acagtaccca atcaacggag cggcgcctta catacttgaa aaagcaatga
     1321 taaccagccg tcaatccagc cacattgtat ttgtaaaggg taccgacgaa aagcgtttaa
     1381 ctcaagtttc tgatctttct aaaaacatgg ctgccggtaa aattgatttt gaagtgcaat
     1441 ccgaaggatt atcaggaata cttcttggtt tgagtgttgc aattaacctt gatgtgagta
     1501 caggcgatac ggtgacagtt attagtcctt ccggggttac agggcctttt agcgctccac
     1561 ccatgcgtcg ttatgtggta acgggtttgt ttaaaaccga tatgtacgag tatgataatg
     1621 cttacgtatt tatatccata ccggaagccc agaaactatt tgaaaaggac gaggcgatta
     1681 ccggcttgga tattcgtttt gagaatattg cacaagcgtc cgaatacgaa aatgcgatac
     1741 gcaccgagat aggtgagagt tttgtatatg aaacgtggta tgatatgcac cgcgacttgt
     1801 atgccgcgat gaaattggaa aaatgggggt cgcttttact actgagtatg attattatgg
     1861 tagccggatt taatatcgtt agcacattga tcatggtcgt tatgcagaaa acacaagaaa
     1921 ttggaattct caaatcaata ggcgctaatc aaaaatttat ttcgtccatc tttgtcagac
     1981 aaggtctgat cgtcgggagc ataggaattg tcgccggatg tattgtaggt tattcgattt
     2041 gttttattca actacaatgg ggtattttta agctgccggg agatattttc tttttggatg
     2101 ccatacctat ggaactccag tggggtgatt tttttgcggt catcgtcata tccgttttat
     2161 tgtgcctttt cgctacgtac tacccc
//
LOCUS       DAOQNQ010000437         2160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_32637_length_2160_cov_4.676485, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000437 DAOQNQ010000000
VERSION     DAOQNQ010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32637_length_2160_cov_4.676485"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="PK754_16025"
     CDS             <1..1104
                     /locus_tag="PK754_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11061.1"
                     /translation="MKHIFILWILAGTMAAHAGAMQSDTIQHIGAEAYQLKMQSIYQA
                     ADSLYRITGSDTENPAIQIMRQAEDELPVHYAVVYSYRMRMGGDSLYTGIMRVDNRYL
                     KRLMQQSIKPDGTSYRDALRTELLDRLYALDELAENGIILDSTDYRVVALRIAADKDF
                     QPEQKTNVFSYFIDKFFEWLRRLFPGNPDTPETKEQTNNADYGWLATILLYSIYAIGL
                     ALLVYIIYWAYKQYRRNTTADETLPATATHSIIEPGESTDYEVHASLATQYAVSGDYR
                     KAIRHLYIALILELDARLILRFDKAYTINEYLTFAKNQSVAPELLRLLIQSSAQFEYF
                     WYGHQTTDRADFERLLLWYNDARNLYNTASHGK"
     gene            1094..>2160
                     /locus_tag="PK754_16030"
     CDS             1094..>2160
                     /locus_tag="PK754_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HNB11062.1"
                     /translation="MANNRLFLSLSIFCGLAILLLLFFSDPGATIHTDSPTSYSVQPG
                     GTKIFYKLLTDNGYNVERWRKKINSVPVAQEAQSIIMIAPSDALSSQETEFLISFIHQ
                     GHTAFLFYSSALDDLLQKLHVRCNTDSSMISFPSGYLGNTVSGDTLHPHIITSDSTTH
                     ARFEDECVYFNSDSSAIIPLYTTLNGHTALEIRHGQGKLIIFNSPNYVTNAGLQLRHN
                     ADIIFRIFQVDPGGNQREPGIIYWDEYHHGYKDYESLIAALDTWPIKWGMILSTISLL
                     FWVHARAKRWGRPVPVITSSRRASIDYVHSVSGVYQNSSAHTLALHHWYRWVMADLQR
                     RYATRDPDKLALILHRRFALD"
BASE COUNT          579 a          531 c          459 g          591 t
ORIGIN      
        1 atgaaacata tttttattct ttggatatta gccggtacga tggccgcaca tgccggcgca
       61 atgcaatccg atacgataca gcacatcgga gcggaagcct atcaattgaa aatgcagtcc
      121 atttatcaag cggctgattc gttgtatcgc ataaccggct cagatacaga aaaccctgcc
      181 atacaaatca tgcgccaagc cgaagacgaa ttaccagttc actatgccgt cgtatatagc
      241 taccgtatgc gtatgggcgg cgattcactt tataccggca tcatgcgcgt tgacaaccgt
      301 tatttgaagc gtttgatgca acaaagtatc aaacccgacg gaaccagtta tcgcgatgca
      361 ttgcgaacag agttgctcga ccgcctgtat gctttggacg aactggctga aaacggaatt
      421 attttggata gcaccgatta ccgtgtagtc gctttgcgaa tcgccgcgga caaagatttc
      481 caacctgaac aaaaaaccaa tgtcttttca tatttcattg ataaattttt tgaatggttg
      541 cgtcgcctct ttcctggcaa tcccgataca ccggaaacaa aagaacaaac caacaacgcc
      601 gactacggat ggctggctac gattttgctg tacagcatct atgcgatcgg tttagctttg
      661 ctagtctata ttatttattg ggcgtataaa caataccgcc gtaacacaac ggccgatgaa
      721 acgttaccgg ccacggcgac acatagcatc atcgaacccg gcgaatctac ggattacgag
      781 gtacatgctt cattggcaac acaatatgcg gtttccggag attaccgaaa agcaatccgc
      841 cacttatata ttgctttgat tttggaactt gacgcccgat tgattttacg gtttgataaa
      901 gcctacacga tcaacgaata tcttacgttt gccaaaaacc aatccgttgc accggagctt
      961 ttgcgattgt tgatacaatc cagcgcacaa tttgaatact tttggtacgg ccatcaaaca
     1021 acggatcgcg ctgattttga gcgcttgtta ctatggtata acgacgcaag gaacttatat
     1081 aatacggcaa gccatggcaa ataatcgttt attcttgagc ctcagcattt tttgcggcct
     1141 agctattttg cttcttcttt ttttttccga cccgggcgcc actatacata ccgattcccc
     1201 tacaagttat tctgttcaac cgggcggcac aaaaatattt tacaagcttc ttaccgacaa
     1261 cggctacaac gtcgagcgtt ggcgaaaaaa aataaactcg gtacctgtag cacaggaagc
     1321 gcaatccata ataatgatcg cgccgagtga cgccctttcg tcgcaggaaa cggagttttt
     1381 aatatcgttt attcatcaag gccataccgc gtttcttttt tatagctcgg ctttggatga
     1441 tctacttcaa aaactgcacg tccgttgtaa taccgattcg tccatgattt cttttccgtc
     1501 gggttatctg ggaaataccg ttagcggcga cacattgcat cctcacataa taacctccga
     1561 ttcgacgacg cacgctcgtt ttgaagacga gtgtgtctat tttaattcag attcgtcggc
     1621 catcattccg ctttatacca cattaaacgg ccatacggct cttgaaatac gtcatggaca
     1681 aggaaagcta attattttca attcgccgaa ttatgtaact aacgccggtt tgcaactgcg
     1741 gcataatgcc gatattattt ttagaatatt ccaagtagat cccggtggaa accaacgtga
     1801 accgggtatc atttattggg atgaatatca tcacggatat aaagactacg aatccctcat
     1861 cgcggctctg gacacctggc cgatcaagtg gggcatgatt ctcagtacga tcagtctttt
     1921 gttttgggtg cacgcacgcg ccaaacgttg gggtcgcccc gtaccggtta tcacttcgtc
     1981 acgacgcgct tcgatagatt acgtgcactc cgtgtccggt gtatatcaaa attcgtccgc
     2041 gcatacgctc gcacttcatc attggtaccg atgggttatg gccgatctgc aaaggcgcta
     2101 tgccacacgc gatccggaca aactcgcgct cattctccat cgaaggtttg cactggatat
//
LOCUS       DAOQNQ010000438         2155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_32789_length_2155_cov_3.854762, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000438 DAOQNQ010000000
VERSION     DAOQNQ010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32789_length_2155_cov_3.854762"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PK754_16035"
     CDS             <1..380
                     /locus_tag="PK754_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020531498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNB11063.1"
                     /translation="YEDIRKALPGIAIVQVVHVTDESSINEAIRVSEFADAVLLDSGN
                     QKLAVKELGGTGRKHDWRISARIRAAIKVPLFLAGGLNSENLREAIETVQPFGIDLCS
                     SVRTDRKLDKTKLERLFEVVHRI"
     gene            complement(471..>2155)
                     /locus_tag="PK754_16040"
     CDS             complement(471..>2155)
                     /locus_tag="PK754_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNB11064.1"
                     /translation="ASGSTWTFTSLSSSGLPAGVTSFAFHPTDVNTAWATIGGFTAGS
                     KVYKTTNGGTSWTNISGSLVNVPANCVAVHPSTPTDVYIGTDLGIFYSADGGTTWEAY
                     DTGLPNVIVTDLDIHVSAGKIRAATFGRGLWESPLNTSTPSNSVTVTAPNGGESLTGG
                     SATTITWSSTGTMANVKLEYSVDGGTNWTVITASTTNDGSEAWTVPSSATTQGRVRVS
                     DASASATNDMSNANFTITVSASNSVTLTAPNGGQSFTGGSATTITWSSTGTIANVKLE
                     YSLNAGSTWTQITASTANDGSEAWTVPSSATTQGRIRVSDAANAATTDMSDANFTITT
                     GTPPITAESESNGTSGTADGPVGTGVAVSGAISSTTDNDYYYFDVSTVGSINISMSIA
                     TANKDLDFFLYNASLTEVARAYTTNNPEVVNYTAAVGRYYIKVNGYNSAQSAYTLTVS
                     GGLAQVTRGGEKGLAGIPQAVELLQNYPNPFNPSTMIRFGLPSENDVTLKIYNLMGQE
                     VRTLFSGRMKAGQNEVLWDGRNAAGQQVSSGTYIYRLQVGQEIISRRMNFIK"
BASE COUNT          536 a          524 c          546 g          549 t
ORIGIN      
        1 cttacgaaga tatccgaaag gctttgccgg ggatcgctat cgtacaagtc gtacatgtaa
       61 ccgacgaatc atccatcaac gaagcgatac gcgtatcgga gtttgccgat gcggtattac
      121 tggattcggg aaatcaaaaa ttggccgtca aagagcttgg cggaactggc cgtaaacacg
      181 actggcgaat cagcgcacgc atccgagccg cgatcaaagt tcctctgttt ttagccggcg
      241 gacttaattc cgaaaactta cgcgaagcta tcgaaacggt gcaacctttt ggcatagatc
      301 tgtgcagcag cgtacgtacc gacagaaaat tagataaaac taagttagaa cgattgtttg
      361 aagtcgtaca tcgcatctga tcaaaacaaa ctcaggttct cagcaggttt ataaaaaaag
      421 cccgatctga aatttcagac cgggcttcgt atttgttcga gcgtgatgaa ttatttgata
      481 aagttcatcc gtcgtgaaat gatttcttgt ccgacttgca ggcgatagat ataggtaccg
      541 ctggaaacct gctggcctgc ggcattgcga ccatcccaca aaacctcatt ttgaccggct
      601 ttcatacgtc cgctaaacag cgtacgtact tcttgtccca tgagattgta gattttgagc
      661 gtgacgtcgt tttcagacgg cagaccaaaa cgaatcatcg tggacgggtt gaacgggttg
      721 ggataattct gcagaagttc gactgcctgc ggaataccgg ccaatccttt ttcgccgcct
      781 cgggttactt gagccaatcc gccgctcacg gtcaaggtat aagccgattg agcgctatta
      841 taaccgttta ctttgatata gtaacgtcca accgcggcgg tataatttac aacttcagga
      901 ttattcgttg tgtaagcacg tgctacttcc gtaagcgacg cattatacag gaagaaatca
      961 aggtctttat tcgccgtggc aatactcatc gaaatattga tggaacctac ggttgataca
     1021 tcaaaataat aatagtcgtt atccgtagtt gatgagatgg ctccggatac ggctacacct
     1081 gtacccaccg ggccgtccgc ggttccggaa gtaccgttac tttcagattc tgccgtaatc
     1141 ggcggcgtac cggtagtgat ggtaaaatta gcatcactca tatctgtcgt ggcggcattt
     1201 gcggcatcac ttacgcgaat gcgcccttgg gtggttgctg aactcggaac agtccacgct
     1261 tcggagccgt cattggctgt ggaagccgtg atctgtgtcc atgtcgagcc ggcattgagc
     1321 gagtattcta gttttacatt ggcgattgtt cctgtagaag accacgtgat cgtcgttgcg
     1381 ctgccgccgg tgaacgattg gccgccattg ggcgctgtca aggtgacact gttggatgcg
     1441 cttaccgtga ttgtaaaatt agcattactc atgtcattgg tcgccgaagc ggatgcatca
     1501 ctgacacgca cgcgaccttg tgtcgtcgct gaagaaggaa cggtccatgc ttcggaaccg
     1561 tcattggtcg tagaggccgt tatcactgtc caattggtac cgccatccac ggaatattcc
     1621 aatttgacat tagccatcgt ccctgtcgag gaccatgtga ttgtcgttgc actgccgccg
     1681 gtaagcgact cgccgccatt gggcgctgtg acagttaccg aatttgaagg tgtggacgta
     1741 ttaagcggcg attcccataa tccgcgacca aacgtcgctg cacgaatttt accggcgctc
     1801 acatgaatat ccaaatcggt aacgataaca ttgggtaatc ctgtatcata ggcttcccat
     1861 gtcgttccgc cgtccgcaga gtagaatata ccaaggtctg ttccgatata gacatcggtc
     1921 ggggtgcttg gatgtacggc gacgcagtta gcaggtacat tgaccaaaga accggaaata
     1981 tttgtccacg aagtaccgcc attggtggtt ttataaactt tgctacctgc cgtgaaaccg
     2041 ccgatggtag cccacgcggt atttacatcc gtagggtgaa atgcaaatga ggtaacacca
     2101 gccggcaaac cgctgctcga caagcttgta aatgtccagg tcgagccact ggctg
//
LOCUS       DAOQNQ010000439         2144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_33093_length_2144_cov_5.438966, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000439 DAOQNQ010000000
VERSION     DAOQNQ010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33093_length_2144_cov_5.438966"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1204)
                     /locus_tag="PK754_16045"
     CDS             complement(<1..1204)
                     /locus_tag="PK754_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB11065.1"
                     /translation="MLLNPILFSSNQDIEVSYKSFRDSLQAGRVDEVQITGEKIFGRF
                     KLSDSAIAAMQTKEAEKPTDLAERIRLGQLFENTFKKDMRQPFVVIALPDEKLVEDLQ
                     KYGVNYKGVIDSNWLENLLLYWVFPLILLFFVWGFIFRRMGGGANVMNIGKNKARIYA
                     ADTKTRVTFEDVAGVEEVIEEVREIVDFLKNPKKYTKLGAKIPKGVLLVGPPGTGKTL
                     LAKAVAGEANVPFFSLSGSDFVEMFVGVGASRVRDLFAEAKSKAPCIIFIDEIDAIGR
                     SRAKGFVMGGHDERENTLNQLLVEMDGFNTDKGVIIMGATNRPDVLDSALLRPGRFDR
                     QVVLDRPDLKARVDIFKVHTKGMPLASDLDVKSLAAQTPGFAGAEIANVCNEASLLAS
                     RKDKERIEM"
     gene            complement(1286..>2144)
                     /locus_tag="PK754_16050"
     CDS             complement(1286..>2144)
                     /locus_tag="PK754_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNB11066.1"
                     /translation="GLKENQIRFTKDAVRMIVREYTREAGLRNLEREMATVCRGVARE
                     VAENKITKKTIDGKNLHEYLGRAKFFLDASERIKKPGIVTGLAWTAAGGDILFIEASK
                     MRGKGALTLTGQLGDVMKESAVAALSQIKSQAEALGITEDFDKIDIHIHVPAGAIPKD
                     GPSAGITMYTALYSLLTDRLVRNDVAMTGEITLRGTVLPIGGVKEKILAAHRAGLRTI
                     ILPDKNEKDLDEVPAQIRKEMTFIPVNEVSKVIENALRPDKRAVHSSHGNGKIKPKKT
                     LRKKIAYSK"
BASE COUNT          574 a          534 c          414 g          622 t
ORIGIN      
        1 tcatttcgat acgttcttta tctttacggg aagccaataa tgacgcttcg ttgcaaacat
       61 tggcgatctc cgcaccggca aaaccgggcg tttgtgctgc caacgatttc acatctagat
      121 cagaagctaa tggcatccct ttggtgtgca ctttaaaaat atccacgcgg gccttgagat
      181 caggacgatc taacacgacc tgacgatcaa aacgcccagg tctgagtaat gccgaatcca
      241 aaacatcagg acgattggtc gctcccatga tgataacacc tttgtccgta ttaaaaccat
      301 ccatctcaac gagtaattgg ttcaacgtat tttcacgttc atcatgcccg cccatcacaa
      361 acccttttgc acgactgcga ccgatagcgt caatctcatc aataaagatg atacatggag
      421 ctttggattt agcttcggca aaaagatcac gcacgcgcga agcgcccaca ccgacaaaca
      481 tctccacaaa gtccgaaccg ctgagactga aaaacggaac attggcctca ccggcaacag
      541 cttttgccaa gagtgttttt cccgtacccg gagggcctac taataagaca cctttgggaa
      601 ttttagcgcc taacttggta tattttttag gatttttgag gaaatccaca atttcgcgta
      661 cttcttcgat aacctcttca actccggcta catcttcaaa ggtaacccgt gttttagtat
      721 cagcggcata tatacgcgct ttatttttgc ctatgttcat cacattggca ccaccgccca
      781 tgcgtcggaa aataaaaccc caaacaaaaa ataaaagtat cagagggaat acccaataca
      841 gtagtagatt ttcaagccaa ttgctgtcta ttacgccttt gtagttaaca ccgtacttct
      901 gcaaatcttc aactaatttt tcatccggta aggcgattac aacaaacggc tgacgcatgt
      961 cttttttaaa cgtattctca aataattgac cgagacgaat acgttctgcc aaatccgtag
     1021 gtttttcggc ttcttttgtt tgcatcgccg ctatggcgct atcggataat ttaaaacggc
     1081 cgaatatttt ttcaccggtg atttgtactt catccacgcg accggcctga agactatctc
     1141 gaaaagattt ataagaaact tcaatatcct ggttggaaga aaacaatatc ggattgagca
     1201 gcataaacac cagcaatatg ccgataatga ctggtatttg gtatttggag ttcattcgac
     1261 taaccggtta cctttctgta tttatttact tcgagtaagc aatcttttta cgtagcgttt
     1321 tttttggctt tatttttccg ttaccatgcg aagaatgaac cgcacgtttg tccggtcgta
     1381 aagcattttc gatgacttta gaaacctcat tcacaggtat aaacgtcatt tctttacgaa
     1441 tctgtgcagg tacttcatca agatcttttt cgtttttatc cggcaaaatg atcgttcgta
     1501 atcccgcgcg atgagccgcc aaaattttct cttttactcc accgattggc agaaccgtac
     1561 cgcgtaatgt gatttcacct gtcatggcga catcgttgcg aacgagtcga tccgtcagta
     1621 aagaatacaa cgccgtgtac atcgtgatac cggccgatgg accatctttg ggtatagcgc
     1681 cggcaggaac gtgaatgtgt atatcaattt tgtcaaaatc ttccgtgata cccaatgctt
     1741 cggcttgcga cttgatttga cttaaagccg ctacggcgct ttctttcatt acatcgccga
     1801 gttgaccggt caatgtaagc gcacctttac cgcgcatttt actcgcttcg ataaataaaa
     1861 tatccccacc ggcagccgtc cacgcaagcc ccgtgacaat acccggtttt ttgatacgtt
     1921 cggatgcatc aaggaaaaac ttcgcgcgac cgaggtattc gtgaagattt tttccgtcaa
     1981 tcgtcttttt cgtaatttta ttctccgcta cttcacgcgc cacacctcgg caaaccgttg
     2041 ccatttcacg ttccaagttt cgcaaaccgg cttcccgtgt atattcgcga actatcatgc
     2101 gaacggcatc tttagtaaaa cgaatttgat tttctttcag accg
//
LOCUS       DAOQNQ010000440         2142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_33146_length_2142_cov_4.843795, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000440 DAOQNQ010000000
VERSION     DAOQNQ010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33146_length_2142_cov_4.843795"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PK754_16055"
     CDS             <1..608
                     /locus_tag="PK754_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11067.1"
                     /translation="IWALALIAQVSSFVVAQSKPEPCMINESFSLAKHKPEKIAQCIE
                     EQYNKSLNSPGTVLYDSKDLNMFFVKGVRLPDVNKNLSSFGKRLYADCFEYRGGPILA
                     FSDDKPDSTDETVRRYVFGSAVVLTDKKGIFEIHCFDSKYEAAKGVKVGMAITEVVAK
                     FPGLKLTNEKEGDVLISNKGCLTVHYTDSIVRWMVVARGCY"
     gene            611..1987
                     /locus_tag="PK754_16060"
     CDS             611..1987
                     /locus_tag="PK754_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNB11068.1"
                     /translation="MSLLLIGILLYVALQLLIGVFVSRKIHSESDYLLAGRSLGMGLA
                     TFSIFATWFGAETCIGTAGAAYESGWAGVKADPFGYGVCVLIVGIFFAIRLWKQKLTT
                     IGDFFRARYSRSVEKTAVILMIPTSIMWAAAQIRAFGLVLSASSELTITLSVTIAAVV
                     VVTYTAFGGLLADAMTDVVQGITLIIGLLVIMPFLISDTGGIEGFMGIILQERHNETN
                     VNFLEIAETWAIPICGSLVAQELLSRVIASRSPQIAQRSSLLAAILYVSVGIIPVLIG
                     LTGFVVLPGLSESESILPMMAQKYLTPMLYILFSGALVSAILSTVDSTLLAASSLMTH
                     NLIIASRRDLDEKLKLRISRWGVIFMGILSYILALYAEGIYDLVKDASALGSAGIFIV
                     FVFGLFTNWGGAYSALASLIVGTAVWIGAHYAWESSTGYLTALGAAFIVYVGIGFFEK
                     HTKPITPI"
BASE COUNT          539 a          408 c          519 g          676 t
ORIGIN      
        1 ggatttgggc tttagctttg attgctcaag tgtcatcatt tgtagtcgcg cagtcaaaac
       61 ccgaaccttg tatgatcaat gaatcgttta gtctggctaa acataaacct gaaaaaatcg
      121 cgcaatgtat cgaggaacaa tataataaat cacttaacag tccgggcaca gtactctacg
      181 atagtaaaga tttaaacatg ttttttgtaa aaggcgtaag attgcctgac gtaaataaaa
      241 atctatcttc ttttggtaag agattatatg cggactgttt tgagtatcgt ggtggcccta
      301 ttttggcgtt ttctgatgac aagccggata gtacggacga aacggtacgc cgttatgtat
      361 tcggttcagc cgtagtactt acagataaaa agggtatatt tgaaatacac tgttttgata
      421 gtaagtatga agctgcaaaa ggagtgaaag tcggaatggc gatcaccgag gttgttgcta
      481 aatttcccgg tttgaaactt accaatgaaa aagaaggtga tgtgttgatt tctaacaaag
      541 gttgcctgac tgtgcattat accgatagta tagtacgttg gatggttgta gcgaggggtt
      601 gttattagtc atgtcactgt tgcttatcgg aattttgttg tatgtagcgc tccaactttt
      661 gatcggcgta ttcgtttcac gcaaaatcca ttcggaatcc gactacctcc ttgcagggcg
      721 gagcctgggt atgggacttg ctacattttc gatttttgcg acttggtttg gtgctgaaac
      781 atgcatcggc acggccggag cggcctatga aagcggttgg gccggcgtca aagcggatcc
      841 ttttggttat ggcgtatgtg ttttgatcgt cggtattttt tttgcaattc gtctgtggaa
      901 acaaaaacta acgacaatcg gtgatttctt tagagccaga tactcacgtt ctgttgaaaa
      961 aacagctgtc atcctgatga tccccacatc gatcatgtgg gctgctgcac agatacgtgc
     1021 gtttggctta gtgttatccg cttcgtcaga acttaccatt acactttctg tgacaatcgc
     1081 cgctgtggtc gttgtaacgt acacggcatt cggaggtctt ttagccgacg ctatgacgga
     1141 tgtagtgcag ggtatcacgc tcattatcgg attactcgtg atcatgccat ttctgatatc
     1201 cgacaccggt gggatagaag gttttatggg tattattttg caggaacggc ataatgaaac
     1261 aaatgtcaat tttttagaaa tagctgagac atgggctata ccgatatgtg gttcgctcgt
     1321 tgcgcaggaa ttgctttcgc gcgtcattgc gagccgctct cctcaaatcg cacaacgttc
     1381 atcgctcctt gcggcgatac tgtatgtcag tgtgggtatt atccctgtgt tgatcgggtt
     1441 gaccggtttt gttgttttgc cgggactatc cgaaagtgaa agtattttgc cgatgatggc
     1501 ccaaaaatac cttactccga tgttatatat tcttttttcc ggagcgctcg tgtctgcgat
     1561 tttatctacg gtggacagta cgcttttggc agcttcatca ctgatgacac ataacttgat
     1621 cattgcgtcg cgtcgcgatt tagatgaaaa gctaaaactt cgtatatccc gatggggcgt
     1681 tatatttatg ggtattttat cctatatctt agcattatat gccgaaggta tttatgattt
     1741 ggtcaaagat gcgtcggccc tcggtagtgc gggaattttc atcgtttttg tttttgggtt
     1801 gtttacgaat tggggtggtg cgtatagcgc tttagcgagt ttgatagtag gtacagccgt
     1861 atggatcggc gctcactacg cgtgggaatc ttcgacgggc taccttactg cgcttggtgc
     1921 ggcttttata gtgtatgtgg gaattggttt ttttgaaaag cacactaagc ccataacacc
     1981 aatttaatta acgaaaatcg ctcttttcag acaccttact gcgtagctcg tttcgaatac
     2041 aaaatgcaaa cataagtgcg attatcatca agaattcaac atattcgtta aatagataaa
     2101 atcggttatt cataaaaccg tggagatagc atgaatcgca gc
//
LOCUS       DAOQNQ010000441         2135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_33333_length_2135_cov_6.125962, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000441 DAOQNQ010000000
VERSION     DAOQNQ010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33333_length_2135_cov_6.125962"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PK754_16065"
     CDS             complement(<1..532)
                     /locus_tag="PK754_16065"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB11069.1"
                     /translation="MNFKVTSSWLYLFVLLLLSGITVVTAQSDIERGRELFKNKRYSE
                     AKPIFEGILNGNEKNAEANYYLGMIYWRYVGDLDKASELLETATENDESNAQYHFLYS
                     GVLGVRAMRSNPIKQAYLATKIRNQLEKTIELEPTHVDARYNLIQFYIMAPGFMGGSI
                     EKAKVQAGALVKYNAAR"
     gene            complement(602..>2135)
                     /locus_tag="PK754_16070"
     CDS             complement(602..>2135)
                     /locus_tag="PK754_16070"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB11070.1"
                     /translation="STEDNYTTIYYLTAITLLMLALACFNYINIAIVSAAKRLKEIGI
                     RKSIGATRSVISVQFLAENLVITLFAMIGGYILATHIFIPGFEYLWHFSMGFEFLDPK
                     LWIFLAVVLVVTSIASGIYPALYISRFHAAGILKGSVRFGKNNPLTKVLLSIQLVLAC
                     MFMTCAVDFTRNTDFLSNRSWGYENREVLYTAVPDRSAFEQLRSKLLQEPNALSISGS
                     THHLGKKHVTTLIQRPERAYEVDQLFVDAHYFQTMGIPISRGRYFPDEAQADQRSIVI
                     NETMVKHLNLENPIGHKLRIDSTEYSVIGVTQDIHVYDFSSAIRPTLFRVASPDITCF
                     LSVKVRPGTQPEMYRILKNHWSSLFPEIPFDGGYQEDVWGRYYETIGIHGLVWRIFAF
                     FAVAMAGLGLYGLITYNVTGRIKEFSIRKVLGAEAFSISALLSRPYTLLYGAALLVGM
                     PLSYLFATHLLVASYPYHMPVSYTGVFIAALALVLVLALSLSIQIHRILKSNPVEGLR
                     TE"
BASE COUNT          627 a          484 c          463 g          561 t
ORIGIN      
        1 cccgcgcagc attatacttg accagcgctc ccgcttgcac cttagctttt tcgatactac
       61 cacccataaa tccgggcgcc atgatgtaaa attgaattag attataacgc gcatccacgt
      121 gtgtcggttc aagttcgatt gttttttcca attgattgcg gatttttgtc gccagatagg
      181 cttgcttaat ggggttactg cgcatggctc tgactcccag aacgccggaa tataaaaagt
      241 ggtattgcgc gttggactcg tcgttttccg tggccgtctc gagaagttca ctcgctttgt
      301 caagatcacc gacatagcgc caataaatca tacccaagta atagttggcc tcagcgtttt
      361 tttcattccc attgagaatt ccttcgaata tgggtttggc ttcgctatac cgcttatttt
      421 taaataattc gcgaccccgt tcgatatccg attgtgcagt aaccacagtg attccggaga
      481 gcagcaggag tacaaacaaa tacaaccaag agctggtaac tttgaagttc acgacgattt
      541 cctttcgatg attattcgta attattcgtt gaatatctta cgaaagccgc atgtaaatgt
      601 tttattccgt tctcaatcct tctacgggat tcgatttcag aatacggtgt atctgaatcg
      661 aaagcgacaa cgccagaaca agaaccagtg caagcgcagc aatgaaaact ccggtatagc
      721 tgacgggcat gtgatacggg tatgaagcga caagcaaatg cgttgcaaac aaataactca
      781 atggcatgcc aactaatagc gctgcaccgt aaagcagcgt ataaggacgc gaaagaagag
      841 ccgatatcga gaaggcttca gcgccaagta ctttgcgaat gctgaactct ttgatgcgtc
      901 ctgtaacatt atatgttatt aatccataca gaccgagtcc tgccatagcc actgcaaaaa
      961 aagcaaatat gcgccaaacc aatccatgaa ttccgatcgt ttcataatac cggccccaaa
     1021 catcttcctg ataaccgcca tcaaagggta tttcaggaaa cagggatgac caatggtttt
     1081 ttaaaatgcg atacatctca ggctgtgtac ccggccgcac tttaacggac agaaaacacg
     1141 taatgtccgg tgaggcaacg cgaaataatg tcggccgtat tgccgaagaa aagtcgtaca
     1201 cgtggatgtc ctgtgttacg ccgatcacgc tatactccgt actatcaata cgtaatttat
     1261 ggccgatggg attttctaaa tttaagtgtt tcaccatggt ctcattgatc acgatactgc
     1321 gctgatcggc ttgggcttcg tcaggaaaat atcggcctct gctgatggga attcccatgg
     1381 tctgaaaata atgcgcatca acaaataatt gatccacctc ataagctcgt tccggtctct
     1441 gaataagcgt agtcacgtgt tttttgccga ggtggtgtgt cgagcccgaa atcgagagtg
     1501 cattaggttc ttgtaataac ttagaccgta attgttcaaa tgcggatcgg tctggaacgg
     1561 cagtgtataa tacttcacga ttttcatatc cccaactgcg gttcgacaaa aaatcagtat
     1621 tccgcgtgaa atcaacggca caggtcataa acatgcatgc cagtaccaac tggatggaca
     1681 ggagtacttt agtgagagga ttatttttac caaaccgcac agagcctttc agaataccgg
     1741 cagcatgaaa ccgtgaaata tacaacgcag gataaatgcc cgatgcgata ctcgtgacta
     1801 ctaaaacgac cgccaaaaaa atccaaagct tgggatctaa aaattcgaag cccatactga
     1861 aatgccagag atattcgaat cccggaataa aaatatgtgt cgcgagaata tagccgccaa
     1921 tcatggcgaa caatgtgatt accaaatttt cagctaaaaa ctgtacggag ataacactgc
     1981 gcgtggcgcc gatagatttt ctaataccga tctctttcaa tcgtttggca gcggatacga
     2041 tcgcaatgtt gatatagtta aagcatgcta atgccagcat caataaagtg atcgccgtga
     2101 gataatagat agtagtgtag ttatcttccg tactg
//
LOCUS       DAOQNQ010000442         2127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_33584_length_2127_cov_4.196911, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000442 DAOQNQ010000000
VERSION     DAOQNQ010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33584_length_2127_cov_4.196911"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..581
                     /locus_tag="PK754_16075"
     CDS             111..581
                     /locus_tag="PK754_16075"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNB11071.1"
                     /translation="MVLKNDRLEKAQGEETKKGDRIFLGYDEKKSVVGFAIPASEPGF
                     ADVIKIIYGYQADGKIIVGFEVLENKETPGLGDKIIKDKNFVANFKALSVEPEIEAAK
                     TGKKTKPNQVETITGATISSKAVIRALQKAMTRWQVAIDGYMQQNNLTYNQKQP"
     gene            578..1204
                     /locus_tag="PK754_16080"
     CDS             578..1204
                     /locus_tag="PK754_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNB11072.1"
                     /translation="MNAIEKSGRATHELIKGLWKENPTFVAVLGMCPTLAVTNSAING
                     LAMGLATTFVLVGSCLLVSMLRHWIPKQVRITTFIVIIATFVTAVDQSLEAFFPKIHK
                     ELGAFIALIVVNCIILGRQEAFSYKNPPWLAVMDALGMGLGFLGALMTLSCIREVLGN
                     GSLFGVSLFGDAFEPWVIMILPPGGFLLLGSLLVVINWIKFGRQTAKV"
     gene            1211..1789
                     /locus_tag="PK754_16085"
     CDS             1211..1789
                     /locus_tag="PK754_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HNB11073.1"
                     /translation="MGEYLWIFISALLINNFTLANFLGICPFLGVSNKIETAFRMGLA
                     NIFVMLITAICAWALNTYVLVYAPYLRLISFIVVIASTVQFLEMVIKKLSPELFRALG
                     IYLPLITTNCAILGIALFQTSKEYGLVQALFYALGGGGGMTLALVIMAGIREETRIMD
                     IPKTLQGTALSLIIAGILSMAFMGFAGLFSSN"
     gene            1794..2024
                     /locus_tag="PK754_16090"
     CDS             1794..2024
                     /locus_tag="PK754_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11074.1"
                     /translation="MELAKYILPVFASAFLCAFWIIVQMIARRMKTKNLFDHKPSCGH
                     CDEDGSCGGDHCEARETLRKKMPWAGSAAPTK"
BASE COUNT          537 a          476 c          534 g          580 t
ORIGIN      
        1 ttttagtggt cgcttatttt tcgacgctac cgatcatcga gcagaataaa gaagaagcgt
       61 tgcaggaagc catctaacgc gtcgtaccgg gttgccgcag ttatgaagta ttggttttaa
      121 aaaacgaccg ccttgaaaaa gcgcagggcg aagaaacaaa aaaaggcgat cgtatatttc
      181 tcgggtacga tgaaaaaaaa tcggtcgtcg gttttgcgat tcctgcatcg gagcccggtt
      241 ttgcggatgt gatcaaaatc atttacgggt atcaggccga cggaaaaatc atcgtcggat
      301 ttgaagtgct cgaaaataaa gaaacgcccg ggctcggcga taaaatcatc aaagacaaaa
      361 atttcgttgc taattttaaa gcgctgtcgg tcgaaccgga gatcgaggcc gcaaagaccg
      421 gaaaaaaaac caaacccaat caggtcgaaa cgatcaccgg tgcgaccata tcgtcgaaag
      481 ctgttattcg tgctttacaa aaagccatga cgcgatggca agtcgccatt gatggttaca
      541 tgcagcagaa caatcttact tataatcaga aacaaccatg aacgcgattg aaaaaagcgg
      601 acgcgcaaca cacgaattga tcaaaggctt gtggaaagaa aatccgacat ttgttgccgt
      661 cctgggcatg tgccctactc tggccgtcac caattcggcg atcaatggat tggccatggg
      721 actggccacc acatttgttc ttgtaggctc atgccttttg gtgtccatgc tgcgtcattg
      781 gatacccaaa caggtgcgca tcacgacttt tattgtgatc atcgccacat ttgtgacggc
      841 ggttgatcaa tcgctcgaag ctttttttcc taaaattcat aaagaactcg gcgcgttcat
      901 cgccttgatc gtggttaact gcatcatact cgggcggcag gaagcttttt catacaaaaa
      961 tccgccgtgg cttgccgtga tggatgcact cggtatggga ctcgggtttc tcggcgcatt
     1021 gatgacgctt tcatgcatcc gtgaagtttt aggcaatggt tctctttttg gcgtatcact
     1081 cttcggtgac gcgttcgaac cgtgggttat catgatattg ccgccgggcg gatttttatt
     1141 gctcggctca ttgttggttg tgatcaattg gatcaagttc ggccgtcaaa cggcgaaagt
     1201 gtaggcgtat atgggcgaat acctctggat atttatttcg gcgctgctga tcaataattt
     1261 tacgttggcc aattttctcg gcatttgccc gtttctcggc gtgtctaata aaattgaaac
     1321 agcattccgt atgggtttag ccaatatttt tgtaatgctc attacggcga tttgtgcatg
     1381 ggcacttaat acctatgtac ttgtgtacgc gccgtatttg cggctcatca gctttatcgt
     1441 ggtgattgcc agcaccgtgc agtttttgga aatggttatc aaaaaactaa gcccggaact
     1501 ttttcgtgcg ctcggtatct atcttcctct gatcacgacc aattgtgcga ttctcggcat
     1561 tgcgttattc cagacgtcca aagaatacgg attggtgcaa gcgttgtttt atgcgctcgg
     1621 cggcggcggc ggcatgacgc tcgctctggt gattatggcc ggcattcgtg aggaaacacg
     1681 catcatggat attcctaaaa cactgcaggg tacggcgctc agtttgatta ttgccggtat
     1741 tttatccatg gcgtttatgg gtttcgccgg attattcagc tccaattaaa attatggaat
     1801 tagccaaata catcctgccc gtttttgcat cggcattttt gtgcgcgttt tggattatcg
     1861 ttcagatgat cgcacgccga atgaaaacaa aaaatctttt tgaccataag ccgtcgtgcg
     1921 gtcactgcga tgaagacgga agctgcggcg gcgatcattg cgaagcgcgg gaaacccttc
     1981 gcaaaaaaat gccgtgggcc ggatcggctg caccgacgaa ataagaacgg agaatttttt
     2041 atgcaccgaa gacaatttct caaaacaagc ggcgccatgc tgctggtatg tgccggagcc
     2101 ggtctgggac cattgctttt atcaaga
//
LOCUS       DAOQNQ010000443         2124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_33667_length_2124_cov_4.997583, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000443 DAOQNQ010000000
VERSION     DAOQNQ010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33667_length_2124_cov_4.997583"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="PK754_16095"
     CDS             complement(<1..1031)
                     /locus_tag="PK754_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNB11075.1"
                     /translation="MKNNNMEETTFTEKEISELEKFGMNSWFVEELLASYKNNSTMVN
                     ESWQDFFDRMSGKPVTAKPAEISNGNGSLIGNIPMPKPAAGEEAVVLRGVGAKIVENM
                     SYSLSLPLATSFRTIPVKILEENRAIINNFLKKSNRGKLSFTHFIGWAIVKALKAHPV
                     MNNAYTVMNGEPHVIRKPQINLGLAVDQEKKDGSRSLIVPNIKNAGAMNFVQFFAAYD
                     DIIRRARANKIEPADFMGTTISLTNPGTVGTVSSNPRLMVGQGAIIATGAIDFPAQYH
                     GMPESNLSAMGISKVMNVTSTYDHRIIQGAESGLFLKKLHELLLGEDGFYDEIFEALQ
                     LPVKPAQWQV"
     gene            1178..2020
                     /locus_tag="PK754_16100"
     CDS             1178..2020
                     /locus_tag="PK754_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11076.1"
                     /translation="MKKLFFADRIKASTLWAQRRYYMTVVMFFFILSDLTAQMPYTWM
                     RSADSLAAQYNAAQAVDAYRHAVQKDSANCEARWKLAMQTIALGNDLPKCEEQEKLYA
                     EAFEWARVAVSCDSSSLAAWLTLATAAERAAMDEFGSRHSVLLAEMRFAAERALALDS
                     LSDEAEHLLGLWNRFVANASWFSRTTARLMGTAYGTAAISRSIEHFQRAIRLHRDEPR
                     HYLELGRTLVLIERWNEADSAFTKAAELPWQDRYAHRHRRDARHYRELLREGRYAELK
                     NSIE"
BASE COUNT          528 a          531 c          515 g          550 t
ORIGIN      
        1 acctgccact gcgccggttt gacgggcaat tgcaaggcct caaaaatttc atcgtaaaaa
       61 ccgtcttcgc caagcaaaag ttcatgcagt tttttgagga aaagccccga ttctgcgcct
      121 tgaataatgc gatgatcgta ggtgctggta acattcatca ctttactaat gcccatcgcc
      181 gaaagattgg attccggcat gccgtggtat tgcgcaggaa aatcaatcgc accggtggcg
      241 ataatcgcac cctgtccgac catgagtcgc ggattggaag aaaccgtgcc tacggtgccg
      301 ggattggtca gcgaaatcgt cgttcccata aaatccgccg gctcgatctt attggcacgc
      361 gcccggcgaa taatgtcatc atacgccgcg aagaactgaa caaaattcat agctccggcg
      421 tttttgatat taggtacgat caatgaacgg cttccgtctt ttttctcctg atctacggca
      481 aggccaagat tgatttgcgg tttgcgtatg acatgcggtt cgccgttcat cacggtatag
      541 gcattattca tcaccggatg cgccttgagc gctttgacga tagcccaccc gataaaatgg
      601 gtaaacgata atttaccgcg attggatttt tttaaaaaat tattaatgat ggcgcggttt
      661 tcttcgagga tttttacggg tatcgtgcga aacgacgtag ccagcggaag gctcaaactg
      721 tacgacatgt tttcaacgat ctttgcgccg acaccgcgca gtacaacagc ctcttccccg
      781 gcggccggtt tgggcatggg aatgttacct atcaaagatc cgttgccgtt agaaatttcc
      841 gccggtttgg ctgttaccgg tttgccagac atacgatcaa agaaatcctg ccaggattcg
      901 ttgaccatcg tgctattgtt tttataactg gccagcaact cttccacaaa ccaggagttc
      961 atgccaaatt tttcaagttc gctgatttct ttttcggtaa acgtcgtctc ttccatgtta
     1021 ttattcttca ttttttcaaa atatatccag atgattttac agggctgtga taataaacca
     1081 aaacgccata aaattcaaat ggaattgtcc tttatccgca caaatccaac gcttgatttt
     1141 ccattaaatt cttgctacca tcgcaaggca tattcgtatg aaaaaacttt tttttgcaga
     1201 tcggataaag gcctcaacct tatgggccca acgacggtac tatatgaccg tcgtgatgtt
     1261 ctttttcata ttgtcagatc tgaccgcgca gatgccttat acctggatgc gtagcgccga
     1321 ttcgctggcc gcccagtaca atgcggcaca agccgttgac gcgtaccgcc acgccgtaca
     1381 aaaagattcg gctaactgcg aagcccggtg gaagctggcg atgcagacga ttgcactggg
     1441 caacgatttg cccaaatgcg aagagcagga aaaactttac gccgaagcat ttgaatgggc
     1501 gcgtgtcgcc gtaagctgcg attcttcgtc gttagcggcg tggttgacct tggccaccgc
     1561 ggccgaacgt gcggcaatgg acgaattcgg ttcgcgccat agcgttttat tagcggaaat
     1621 gcgttttgcg gcagaacgtg cgctggctct ggattcgctc tccgatgaag ccgaacatct
     1681 tctcggtttg tggaatcgtt ttgtcgccaa cgcttcgtgg ttttcgcgta cgacagcccg
     1741 tcttatggga acagcctatg gcacagccgc catatcgcgc agtatcgaac actttcaacg
     1801 cgcgatacgt ttgcatcggg acgaaccgcg gcattatttg gaattgggac gcacgctggt
     1861 gctgatcgaa cgatggaatg aagccgattc ggcatttacc aaagcggccg aactgccctg
     1921 gcaggaccgc tatgcgcacc gtcatcgccg cgatgcacgc cactatcgcg aacttctgcg
     1981 cgagggccgg tatgcggaat tgaaaaatag tattgaataa cataacggat tgatttgaag
     2041 gagtatcccc atgactattc gcgtgaccaa cgccgaaaac ggcatcaacg aagaatttaa
     2101 aagcaataac ctcgacttta tccg
//
LOCUS       DAOQNQ010000444         2121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_33758_length_2121_cov_4.176186, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000444 DAOQNQ010000000
VERSION     DAOQNQ010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33758_length_2121_cov_4.176186"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="PK754_16105"
     CDS             complement(<1..992)
                     /locus_tag="PK754_16105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11077.1"
                     /translation="MAMQDQQQQQIALNQLMELVQAPDFPGKLVPEHVTQFRSRLERF
                     SETEISAVTERIQEEIEKKRSSNKTLLVSKLEDVAALTKMELVLAEHKVPSHMINLLL
                     TLLVNKSSNFGTPIFDGKMEEFINDSIENAVLNIVLEKAASERTKGDKSGKGKELKEL
                     FEKKIRDTETLTHLIHIIDNVDLNNDATMKCVFVVNLVAEAMKREETMRKSESITMKD
                     MINNYLLSKLQMRLAEYNYPNDTLEGIIINRLGQSNNIKRQFRILDHIIETTLSGDLN
                     IELGSKTEKLEWLQLINSLEINVALAQYEEKLAQDNFPTLDDEIKEEIRRVLAA"
     gene            1139..1795
                     /locus_tag="PK754_16110"
     CDS             1139..1795
                     /locus_tag="PK754_16110"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNB11078.1"
                     /translation="MFKLIHHIFDQSVNFIDINNMSVIFSEKELEKRKALIKVTYKLI
                     AEHGFTNITLQDVADRADVSKGIILYYFENKEALFEAVLEWLVKRIELHIRAEVSRAE
                     DPISKIKAFNDATFLGIKENREFYLVYLDFLAQSSHTERLRNVNLAFYNQCSQVQSQI
                     LRDGIEKGIFREVNTSEYCIISRALIDGLCIRWLFDTPERFTFYKQTALQITLDYLKK
                     "
     gene            1847..>2121
                     /locus_tag="PK754_16115"
     CDS             1847..>2121
                     /locus_tag="PK754_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11079.1"
                     /translation="MTVIITTLGVIILIIIAMLLLTRYMAHKFLTVKLRPKTKTPADF
                     QLPYESVQFESEGRLLHGWLIRNNPQMPLVILVHGWESNAQGMLPHA"
BASE COUNT          625 a          424 c          381 g          691 t
ORIGIN      
        1 tccgccgcca gtacacgacg aatttcctct ttgatctcat cgtcgagggt aggaaaatta
       61 tcttgtgcca gcttttcttc gtattgtgcc aaggcaacat tgatttcaag tgaattgata
      121 agctgtaacc attctaattt ctcggttttt gaaccaagtt caatattcag atcgccggac
      181 aatgtcgttt cgataatatg gtcgagtatt cgaaattgac gtttgatatt attcgattgc
      241 cccaaacgat tgatgataat gccttccagc gtatcgttag gataattgta ttccgctaaa
      301 cgcatctgca atttggataa aagataatta ttgatcatat ccttcatcgt tatggattcg
      361 cttttgcgca tggtttcttc gcgtttcatc gcttcagcta ccagattgac cacaaaaacg
      421 cacttcatcg ttgcgtcgtt attgaggtca acattatcaa ttatatgaat caaatgcgtt
      481 agcgtctctg tgtcacgaat ttttttctca aaaagttctt taagttcttt tccctttcca
      541 cttttatcgc ctttggttcg ttcggaagcc gctttttcca atacgatatt gagtacggca
      601 ttttcgatgg aatcgttgat aaattcctcc atcttaccgt caaaaatcgg agtgccgaaa
      661 ttagaagatt tattgactaa aagtgtcaac aacaaattga tcatatgtga aggcacttta
      721 tgttcggcaa gaaccagctc catcttcgtt aaagccgcaa catcttcgag cttgctgacc
      781 aataaagttt tattagatga tcgctttttt tcaatttcct cttgtattcg ctcggtcacc
      841 gcactgattt ctgtttccga aaagcgttca agacgcgaac gaaattgcgt cacatgttca
      901 ggaaccagtt tgcccggaaa atccggagct tgaacaagtt ccatcaactg gttcagtgca
      961 atttgttgtt gttgctggtc ttgcattgcc ataggttatt ccatggtatc ggtgatttta
     1021 atcatttaaa aagttaacac aatcccgaat acatagcaag caaattagct ataagcctga
     1081 cattacagac gatctctgca tcctatgaac aggctcccaa atttcaaatt aattccttgt
     1141 gtttaaatta attcatcata tatttgacca atcagtcaat tttatagata tcaacaatat
     1201 gtccgtaata ttcagtgaaa aagaactcga aaagcgtaag gccctaatta aggtaacgta
     1261 taagttgatt gctgaacacg gttttacaaa tattacactt caagatgtag ccgatcgtgc
     1321 tgatgtgagt aaaggcataa ttttgtacta ttttgaaaat aaagaagctc tatttgaagc
     1381 tgtacttgaa tggttggtca aaaggatcga attgcatata cgtgccgaag tttcacgtgc
     1441 cgaagatcct atttctaaaa ttaaagcatt taacgatgct acttttttag gaattaaaga
     1501 aaatcgagaa ttttacttgg tgtatctgga ttttctagca caaagcagcc ataccgagcg
     1561 tcttcgtaat gtcaaccttg ctttttataa tcaatgctca caagtacaat ctcaaatttt
     1621 acgcgatggt attgaaaaag gaatcttccg cgaagtaaat accagcgaat attgcatcat
     1681 atcacgtgct ttgattgatg gcctttgtat tcgatggtta tttgatactc cggagcgatt
     1741 tactttttat aaacaaactg cacttcaaat aacactcgat tatcttaaga aataattttt
     1801 tactaaaaaa ctgaccgtca ggtcaaatac taaaaacgga gattgtatga ctgtcatcat
     1861 aacaacttta ggagttatta ttttaattat aattgctatg ttgcttctca cacgttacat
     1921 ggctcacaaa ttcctgacag tcaaactacg cccaaaaacc aaaacgccgg cggattttca
     1981 actgccgtat gaatccgtgc aatttgaaag cgaagggcgt ttactccacg gttggttaat
     2041 tcgtaacaat cctcaaatgc cattggttat tttagttcat ggctgggaat ccaatgctca
     2101 gggcatgctt ccccacgcac a
//
LOCUS       DAOQNQ010000445         2114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_33997_length_2114_cov_3.987373, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000445 DAOQNQ010000000
VERSION     DAOQNQ010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33997_length_2114_cov_3.987373"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PK754_16120"
     CDS             <1..978
                     /locus_tag="PK754_16120"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNB11080.1"
                     /translation="QVPLSAMEMVIDMEADRFYNLALPENMVTSEKEVIKEEKRLRYE
                     DAPMGKLYEHVLDLAYENHPYKNLVIGSWDDLDRIGRDDLMAYYRQQYVSNKMTLVIS
                     GDGDPDAWQVMAERYFGTPKEGPSFSTEVRLTEPARSLARRRVVNKEVALPAIHIAFS
                     AVHVDHPDFAALSFLSGVLATGKSSRLYRHLVYDPQKATSVSCSMDEKKEDGLFHVTA
                     QARPEIAIEELEQALWNEMNKLKTELITLREWERMRNIIRSEWAQSLETTLGRAQWIG
                     RYTTISGRYHNGQLDTLENDFMRVSPEDIRRVAQSYLIPEKSNTVILKP"
     gene            1012..>2114
                     /locus_tag="PK754_16125"
     CDS             1012..>2114
                     /locus_tag="PK754_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11081.1"
                     /translation="MKTIFFCICLLLGAHHPAVTQSLHIVATGDTKGYLFEENVEFGK
                     SGGILERQAAINQLQSAYGRQNMLLFDTGDALGYYYLTTASHGRIVLDKMRDIGYDAM
                     VVGNHEFDFGKDVLKAYVDDPSYPSILGANVLGADSLPYLQPYKIVEKAGLRVGIIGV
                     TDPAITQSVLEEKMAGIIIADPSAAIRAYIDEVREQSDAIVLLTHLSEEENNDFAREF
                     PEINVIIGKSSYIAPDRFSSVRVSDRFETFVFSVDKFGRNIPHITMEVQDRKIHDVQS
                     VEVIELASETIYVDPAMQAAFEEEVELAYAEHCETAYGLGPSSSILKKNWMGNKDLRD
                     YMMAMMLFKANAEIAVINDRFFDFDNYIANAVQT"
BASE COUNT          568 a          437 c          551 g          558 t
ORIGIN      
        1 caggtgcctc tttcggctat ggaaatggta atagatatgg aagccgatcg gttttataat
       61 cttgcgcttc cggaaaacat ggtaacgtcg gagaaagaag tcatcaaaga ggaaaaacgc
      121 ttacgctacg aagatgcacc gatgggtaaa ttgtatgaac atgtacttga tcttgcgtat
      181 gaaaaccatc cttataaaaa tttagtgatc ggttcatggg atgatcttga tcgtatcggt
      241 cgcgatgatt tgatggcgta ttaccgacag caatatgtat caaataaaat gacgctcgta
      301 atttccggtg atggcgatcc ggatgcatgg caagttatgg ccgaacgata ctttggtacg
      361 ccgaaagaag gaccgtcttt ttccacagag gtacgactta ccgagcctgc ccgatcgctt
      421 gcgcgacgcc gcgtagtaaa taaagaagta gcgttgcccg ccatccacat cgctttttca
      481 gccgtccatg tggatcatcc ggattttgcg gcgctgagtt ttttgagcgg agtgctcgcg
      541 acggggaaaa gttcaagact gtatcgtcat ctggtgtatg atccgcaaaa agctacttcc
      601 gtatcatgca gcatggatga aaaaaaggaa gacggccttt ttcatgttac ggcgcaagca
      661 cgcccggaga tagctattga agaactggaa caagcgttgt ggaatgagat gaacaaacta
      721 aaaacagaat tgatcacatt gcgcgaatgg gagcgtatgc gtaatattat ccgttccgag
      781 tgggcacaaa gcttggaaac gacattggga cgcgcacaat ggattggtcg ctatacgacg
      841 atctccggac ggtatcataa cggacaactg gataccttgg aaaatgattt tatgcgcgta
      901 tcgccggagg atatcagacg ggttgcgcag tcctatctta tacctgaaaa atcaaatacc
      961 gtaatattga agccttgatg gtgctaccac ttaacggatt cggtagtttt tatgaagact
     1021 atattttttt gtatatgcct tttacttggc gcgcatcatc ccgccgtcac gcagagtctg
     1081 cacatcgtcg ctaccggcga taccaaaggt tatctttttg aagagaatgt cgaatttggc
     1141 aaatcgggcg ggatactgga acgtcaggct gcgataaacc agctccaatc ggcttatggt
     1201 cgtcagaata tgctcctttt cgataccggc gatgcgctgg ggtattatta tttgacgacg
     1261 gcttcgcacg gtcgcattgt tttggataaa atgcgggata tcggctatga cgcgatggtg
     1321 gtgggtaatc acgaatttga ttttggaaaa gatgtgctca aagcgtatgt ggatgaccct
     1381 tcgtatccgt ccattctcgg cgccaatgtt ttaggcgcgg actcactgcc gtatttgcag
     1441 ccgtataaga tcgtcgaaaa agccgggctg cgcgttggca ttatcggcgt gacggatccg
     1501 gcaataacac aaagtgtact tgaggaaaaa atggccggca ttatcattgc cgatcccagt
     1561 gcggcgatcc gtgcgtatat agacgaagtg cgcgaacaat cggatgccat tgttcttttg
     1621 acgcatcttt cggaagaaga gaacaatgat tttgcacgcg aatttccaga aatcaacgtg
     1681 attatcggta aatccagcta tatcgcaccg gatcgtttta gttcggtacg cgttagtgat
     1741 cgttttgaga cgtttgtgtt ttcggtggat aaatttggcc gcaacatacc gcacattacg
     1801 atggaagtgc aagaccgcaa gatacatgat gtacagagcg tcgaagtgat cgaactggcc
     1861 tccgaaacga tttacgtgga tccggcgatg caggcggcgt ttgaagaaga agtggaactg
     1921 gcctatgccg aacactgtga aaccgcgtat ggattgggcc cgtcgtcttc tattttaaaa
     1981 aagaattgga tgggcaataa agatctgcgc gattatatga tggcgatgat gctttttaaa
     2041 gctaatgcgg agatcgccgt gatcaatgat cgcttttttg attttgataa ttatatcgcc
     2101 aatgccgtac aaac
//
LOCUS       DAOQNQ010000446         2113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_34023_length_2113_cov_6.864431, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000446 DAOQNQ010000000
VERSION     DAOQNQ010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34023_length_2113_cov_6.864431"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1611)
                     /locus_tag="PK754_16130"
     CDS             complement(<1..1611)
                     /locus_tag="PK754_16130"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNB11082.1"
                     /translation="MRRLILVLIFICTGIMLLSWLSHSTTTTYGYVVRPTNRQPIANA
                     RVREIGSSDFVLTNASGLFALTTNTSPGDFLNVSAGKEGWYNVRKIMTVGITDTIFLD
                     PLTDEDNSEYTFQDPNFCDHCHTMLHDQWRTSKHARAATNPMFKQMYTGKDALGNANV
                     YPGFKMDFPSQGGDCGDCHAPSAALQSPGNTFMEDVWAQGGVDTNGVHCDFCHKVQSV
                     EVNYKTGVNGSLFVLRPQAHARDINFGPYDDVTTVWMGGTYNPVYEQSAYCSGCHQYA
                     NKFDVIVDDTYDSWAASTYADQGIQCQHCHMRPNSDSTFVNGISSVDAVVRDTNRVFS
                     QFFQGTTEAFLKSAAKLDVDFTLSANRVTLSSRVTNRAAGHKLPTGVSFRNMLLVVEV
                     KDGFENLEFLTGDTLPTYAGKGSSANGNFSGLPGKGYAMLLRDGSGQMPAPNWSATEI
                     IEDTRLPAKATDTVRSMFRIGRRTSAQVHVRLLYRAVFKAWADAKSWDMREFLMADTT
                     FTVDFDKPYQEPERVALLPNAPNPFNGST"
     gene            1983..>2113
                     /locus_tag="PK754_16135"
     CDS             1983..>2113
                     /locus_tag="PK754_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010624863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11083.1"
                     /translation="MAKTKSYFVCQSCGYKSSKSLRRCPECMTWNSMVEEQSATESSS
                     "
BASE COUNT          551 a          508 c          536 g          518 t
ORIGIN      
        1 cgtacttccg ttgaaaggat tgggtgcatt gggaagtaaa gcgacacgtt ccggttcttg
       61 ataaggttta tcaaaatcaa ctgtaaacgt cgtatcggcc atcaaaaatt cgcgcatgtc
      121 ccatgatttg gcgtcagccc atgccttgaa tacggcacga tacaaaaggc gaacgtgtac
      181 ttgagcggag gttcttcgac cgatacggaa catcgatcgg acggtatcgg tcgctttagc
      241 cggtaaacgc gtatcttcga tgatttcggt tgctgaccaa ttaggcgccg gcatctgacc
      301 gctaccatca cgcaaaagca tggcgtatcc tttgcccggc aaacctgaga agtttccgtt
      361 ggccgacgat cctttgccgg cataggtcgg cagggtatca ccggtgagaa actccaggtt
      421 ttcgaagccg tctttgactt caacgaccag caacatattt cgaaaactca cacccgtcgg
      481 caatttatga ccggcggcgc gattggttac gcgtgaggat aatgtcacgc gattggcact
      541 gagtgtaaaa tccacatcaa gttttgccgc ggactttaga aaagcctccg tcgtaccctg
      601 aaaaaattgg ctaaacaccc gattggtatc gcgtactacg gcatctacgc tggatatgcc
      661 gttgacaaac gtcgaatcgc tattggggcg catgtgacaa tgttggcatt ggatgccctg
      721 atcggcgtat gtactggccg cccacgaatc atacgtgtca tctacgatga catcaaattt
      781 attggcgtac tgatggcaac cggaacagta agcactttgt tcatataccg gattgtacgt
      841 gccgcccatc catacggtag tcacatcatc gtagggaccg aaattgatat cgcgcgcgtg
      901 tgcttgagga cgcaaaacaa ataacgaacc gttcacaccg gttttgtaat tcacttctac
      961 gctttggact ttgtgacaaa aatcgcaatg gacgccattg gtatccacac cgccttgcgc
     1021 ccaaacatct tccataaatg tattgcccgg cgactgtaac gcggccgatg gagcgtggca
     1081 atcgccacag tctccacctt gcgatggaaa atccatttta aaacccggat acacgttggc
     1141 attgcccagc gcgtctttgc cggtgtacat ttgtttgaac atcggattgg tggcggcgcg
     1201 ggcgtgtttg gacgtgcgcc actgatcgtg caacatcgta tggcaatgat cacagaaatt
     1261 aggatcctga aacgtgtatt ccgaattatc ttcatcggtc aggggatcaa ggaatatcgt
     1321 atccgttata ccgacggtca tgatttttcg aacattgtac caaccctctt tacctgcgga
     1381 cacattgaga aaatcgcccg gactggtatt ggttgttaag gcgaaaagtc cggatgcatt
     1441 ggtcagaaca aagtccgatg atccgatctc acgtacgcgg gcattggcaa taggctgacg
     1501 attggtaggt cgtacgacat aaccgtaagt cgtagtggtg ctatgagata accaagacag
     1561 cagcatgatg cctgtacaaa taaaaattaa aacgagaatc aatcttcgca tacgatttag
     1621 aatccctaag atagaactgg tactaggagc cattaaaggt acatgtctgt catctttagc
     1681 gcaatattat tttgaatggg ttttttagtt tagagtttat agactaaaaa gtagcaggat
     1741 taatataaaa aagcggtgtc tcgcgtgagg gatgcgcagg gtacgcggcg ccgccggcgc
     1801 gcgtagccca cgcaaagcgc caaatgcgct gcggaggccg agagcccgaa gccagcccga
     1861 cctgacacgc cacaggcgtg aaggggcacg cccaagtgat cggccgtcgt atcggtgtgt
     1921 ttttagtaga aattctcgta ctatcgtatt agattgcgcc gcatttgtaa aacagcggtg
     1981 tcatggctaa aaccaaatct tactttgtct gccaatcatg cggctacaag tccagcaaat
     2041 cgctcagacg ctgtccggag tgtatgacgt ggaattcgat ggtcgaagaa caatcggcta
     2101 cggaatcgtc ctc
//
LOCUS       DAOQNQ010000447         2105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_34305_length_2105_cov_4.467805, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000447 DAOQNQ010000000
VERSION     DAOQNQ010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34305_length_2105_cov_4.467805"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PK754_16140"
     CDS             <1..186
                     /locus_tag="PK754_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11084.1"
                     /translation="QGRWEERFEDLPAETLMIFIFDADTLDQVPWEKVRSEYKILRRY
                     DLSLQDLKDRDWKVVYP"
     gene            233..811
                     /locus_tag="PK754_16145"
     CDS             233..811
                     /locus_tag="PK754_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11085.1"
                     /translation="MKFITWLFLTISGSIAWAGTLDTISIGFSIGRNSYKKDQKEFFI
                     KTEIRLLNHSFFSKLGVQSRQYDLSFDGVRNLKAKSLGLFAEYIRYLWDDNLFAGMRW
                     ELINFNRLTTASRDRVKREHSYDMTSFYTGTCIFLQIGFDLYVTKNLAITIYPQYGFQ
                     QFMISNAESKPVKEDHYKAIYDVNIGIVWGSK"
     gene            complement(934..1434)
                     /locus_tag="PK754_16150"
     CDS             complement(934..1434)
                     /locus_tag="PK754_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNB11086.1"
                     /translation="MKYLPRRKFLSISAAGIPLFLTSKTALLSQTKKEKGPALDPALV
                     KDFVAIAHKDTTKTIAMLDTNPDLLNAVHNLGGWDWEDALGAAGHVGNPELATALIDR
                     GARMTICVAAMLGKIQIVRPMIEAFPAMKKAVGPHNISIFRHAKAGGDNAREVLDYLV
                     EIGVKE"
     gene            1647..>2105
                     /locus_tag="PK754_16155"
     CDS             1647..>2105
                     /locus_tag="PK754_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB11087.1"
                     /translation="MFRNFYVIALRNLMKNKLFSFINVFGFSLSLSVCLLIISITIDQ
                     YSYDQFHPLGEDTFRINTIAQRKNGDHESYASSPAPLASLIRSENPFVRHTVSLNRSL
                     NGSMTSDDIKLMINGYFTESSFFEVFGFTLLKGNADALNDPRSLMISEETG"
BASE COUNT          629 a          394 c          431 g          651 t
ORIGIN      
        1 caaggaagat gggaggaacg ttttgaagac ttaccagcag aaacgctcat gattttcata
       61 tttgacgcag atacattaga tcaagtaccg tgggaaaaag ttcgttcaga atacaagata
      121 ctgaggcgtt atgacctgag cttgcaagat ttaaaagatc gggattggaa ggtcgtttac
      181 ccttaaattc aaggggctta ttgagttaat aatcaaaagg gaaaaagaac acatgaaatt
      241 tatcacatgg ctgtttctca ctatatcggg aagtattgca tgggccggaa ctttggatac
      301 gatttctata ggattttcaa tcggtcgtaa cagttataaa aaggatcaaa aggagttttt
      361 cataaaaacg gaaattcggc ttttgaatca ctcttttttc agcaaattgg gagtccaaag
      421 ccggcaatat gatttatcat ttgatggagt tcggaatctc aaagccaaat cgctcggttt
      481 atttgcggaa tatattcgtt atctttggga tgataattta tttgccggaa tgcgttggga
      541 gttaatcaat tttaaccgac ttactacggc ctcacgcgat cgtgtgaaaa gggaacactc
      601 ctacgatatg acatcatttt acaccggaac gtgtatattt ttacagattg gtttcgatct
      661 gtatgtgact aaaaatctgg cgatcacgat ttacccgcag tacgggtttc agcagtttat
      721 gatatctaat gcggaatcca aaccggtcaa agaagatcat tataaagcaa tctacgatgt
      781 taatataggc atagtatggg gcagcaaata acataaatct tctctgaata gacgtactcg
      841 ctattttgat gcgaatcaaa aaaaagatat aaaagtgtgc tgcgtgaaat aacgaaagga
      901 aaattctatc tataaaatta ccatagttgg tctttactct tttactccga tttcaaccaa
      961 gtagtctaga acttcccgtg cattgtcacc gccggctttt gcatgcctga aaatggatat
     1021 gttatgcggc cctacggctt ttttcatcgc gggaaaagct tcgatcatag ggcgtacgat
     1081 ttgaattttc cccaacatcg ctgcaacaca tatagtcatc ctcgcgcccc gatctatcaa
     1141 tgccgtagcc aattccggat taccgacatg tcccgcagcg cccagtgcat cttcccaatc
     1201 ccatccgccg agattatgta cggcattcaa aagatcagga ttagtgtcca gcatagctat
     1261 ggttttggta gtatctttgt gggctatagc tacaaaatcc tttaccagcg ccgggtccaa
     1321 ggcaggccct ttttcctttt tagtctgtga caaaagagcg gtcttgctcg tcaaaaacaa
     1381 gggtattccc gctgctgaaa tggacagaaa ttttctgcga ggtaagtatt tcataattat
     1441 cctttcgaga atatatgaag acaatactgt tcggagaggt aaaataaaaa cgaagacagg
     1501 taataactct gaacgtaaaa gactttaaat tgtttcacaa attatatggt gatgttcaat
     1561 acatttgaat tattgcaact ttttggcttt aaacccgtca tatttatatt accggtagta
     1621 tttccaaatt tcgtgaaagg gtagatatgt tccgaaattt ttatgtaatt gctttgcgca
     1681 atctgatgaa aaacaaattg ttttcgttta taaatgtttt tggtttttca ttgagcctgt
     1741 cggtgtgttt actgattatt tctattacta tagatcagta ttcatatgat caatttcatc
     1801 ctttaggtga agatacgttt cgcattaata caattgccca acgtaagaat ggggatcacg
     1861 aatcgtatgc ttcatcgccg gcgccgcttg cttctctgat tcgttctgaa aatccgtttg
     1921 tcaggcatac cgtaagcctc aatcgcagcc taaacggttc tatgacgagc gatgatatca
     1981 aacttatgat caacgggtat tttacggaat cttcattttt tgaagttttc ggatttacac
     2041 ttttgaaagg aaatgccgat gcattaaacg atccccgaag tttgatgatt tcagaagaaa
     2101 caggg
//
LOCUS       DAOQNQ010000448         2077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_35258_length_2077_cov_4.398121, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000448 DAOQNQ010000000
VERSION     DAOQNQ010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35258_length_2077_cov_4.398121"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PK754_16160"
     CDS             complement(<1..213)
                     /locus_tag="PK754_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017483465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HNB11088.1"
                     /translation="MNLWIRMLRVFWKSFFCKKKISLFDESRLSFRVWPHDCDINMHL
                     NNGRYLTLMDLGRTYLMAEMQMLWKLP"
     gene            complement(292..879)
                     /locus_tag="PK754_16165"
     CDS             complement(292..879)
                     /locus_tag="PK754_16165"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HNB11089.1"
                     /translation="MAKKKSAPKKAAKKVVKKAVKKVAKAAPKKSTKKVAKKAAGVKK
                     QKLTPDQLHAKVLGKVETYIKRIYGKSYEKHDDGRFLVFEGSTVVQTVLRPWHAGDIV
                     VESFAYVVQDARLTDELMKFLLRENATLHFGSFGLSFDGTIIFGHSLAGANLDENEFS
                     ASIKSVARIADHYDNKIVEIAGGMTAREAMKKGIM"
     gene            complement(977..1918)
                     /gene="queG"
                     /locus_tag="PK754_16170"
     CDS             complement(977..1918)
                     /gene="queG"
                     /locus_tag="PK754_16170"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNB11090.1"
                     /translation="MADKKLKEFICEEARNLGFDKVGFATAAHVTQAEYLKRWLNQGY
                     HASMQWMNNHLEKRIDPRMLFPGARSVIVVALNYHQPQARTVIENTGKISQYAWGEDY
                     HEVLKDKLYMLLNKLRLHDTQIEGKVCVDTAPMMDKYWAVQAGIGWQGKHSNVISREL
                     GSWFFIGSLIINADIEPDMPIADFCGSCTRCIDACPTQAITHPYVVDSGRCISYYTIE
                     HRGEQLPDSLVAHLQNWIYGCDVCQDVCPWNIKFEKPTREVRFTPRMENINVSLQTWQ
                     NLTEHDFRLKFRKSAVKRTKFAGLRRNIYAALEPKAY"
BASE COUNT          581 a          483 c          391 g          622 t
ORIGIN      
        1 gggtaatttc catagcattt gcatctccgc catcagatat gtacgcccaa ggtccatgag
       61 tgtaaggtag cgcccgttat tgaggtgcat attaatgtcg cagtcatgtg gccacactct
      121 aaaactcaac cgactttcat cgaaaaggct tattttcttt ttgcaaaaaa aagatttcca
      181 aaacacacgc aacatacgaa tccacaaatt catgagctat cccctgatat ataaaaatca
      241 aaaccagcgc cggagacgct ggtttttttc aacgtaatat tttatttcag atcacatgat
      301 gccttttttc attgcttcac gtgcagtcat accgccggca atttcgacga ttttattatc
      361 atagtgatcc gcgatacgtg ctacggattt gatcgaagcg gaaaattcgt tttcatccaa
      421 attggcgccg gccagcgaat gaccgaagat aatcgttccg tcaaatgaca aaccgaatga
      481 accgaagtgt aatgtcgcat tttcacgcaa caaaaacttc atcagttcgt ccgttaagcg
      541 cgcatcctga accacataag caaacgattc gacgacaata tcaccggcgt gccacgggcg
      601 caacactgtt tgaacaaccg tagagccctc aaatacgagg aatcggccgt catcgtgttt
      661 ttcatagctc ttaccataga tacgtttgat atatgtctct actttaccca atactttggc
      721 gtgtaattga tccggagtta acttttgctt tttgactccg gctgcttttt tagcaacttt
      781 cttcgttgat ttttttggtg cagccttagc tacttttttg actgcttttt ttacgacttt
      841 tttagccgct ttttttggcg ctgatttttt ctttgccatg agatcgtgcc tccgccgtat
      901 tgaagtaatt gtattctaaa aattattgac ctgaaaacaa tgattggatc aaaaatcgaa
      961 aaaaaaacaa acaaagtcaa tacgctttcg gttccagagc tgcataaata tttcttcgta
     1021 accctgcaaa tttagtacgc ttgaccgcgc ttttgcgaaa ttttaagcga aaatcatgtt
     1081 cagtcaaatt ttgccaagtc tgtaaggaaa cattgatgtt ttccatacgt ggagtaaaac
     1141 ggacttcccg tgttggcttt tcaaatttga tattccacgg gcaaacatcc tgacacacgt
     1201 cgcagccgta tatccagttt tgcaaatgcg caacaaggct atcaggcaac tgctctcctc
     1261 ggtgttcgat cgtatagtat gagatgcatc gcccggaatc tacaacatac ggatgggtaa
     1321 tcgcttgcgt gggacatgca tcaatgcatc gcgtacatga tccgcaaaaa tctgcgatcg
     1381 gcatatctgg ctctatatca gcattgatga tcagcgatcc gataaaaaac caagaaccca
     1441 attcacgcga aataacattg gaatgtttgc cttgccatcc gatacctgcc tgtacggccc
     1501 aatatttatc catcatcggc gccgtatcaa cgcatacctt gccttcgatc tgcgtatcgt
     1561 gcaaacgaag tttattaagt agcatataca gtttatcttt taacacctca tgataatctt
     1621 cgccccatgc gtattgggaa atttttccgg tattttctat cacggtacgt gcttgaggct
     1681 ggtgatagtt aagtgccacg acgattacgg aacgcgcacc tggaaacaac atgcgcggat
     1741 caatgcgttt ttctaagtgg ttattcatcc actgcatgga cgcatggtaa ccttgattca
     1801 accaacgctt tagatattcc gcctgtgtaa cgtgagccgc tgtagcgaat ccgactttat
     1861 caaaccccaa attccgggct tcttcacata taaactcttt taatttttta tcggccataa
     1921 gttttatagc tattggtcat ctaatataca ccgatatcaa aaaaaatcgc atatgaattt
     1981 catatgcgat aaatatgcta atatatgaat cggccctcaa ccgaatgttt ttggattttc
     2041 cctgtgccgc cgcatacgta aaaaaacaaa cctgatc
//
LOCUS       DAOQNQ010000449         2057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_35921_length_2057_cov_6.629371, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000449 DAOQNQ010000000
VERSION     DAOQNQ010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35921_length_2057_cov_6.629371"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="PK754_16175"
     CDS             <1..129
                     /locus_tag="PK754_16175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB11091.1"
                     /translation="TKKHGTGLGLPIVKSIVEAHRGKVQIETRIGSGTTFIFTFPK"
     gene            131..1021
                     /locus_tag="PK754_16180"
     CDS             131..1021
                     /locus_tag="PK754_16180"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNB11092.1"
                     /translation="MTVRLILFFSLTISLHAGSGETGFAFLKINASARSSAMGEVSTY
                     DGQDPMGVLVNPANLSVTPSHSVAFTYNRWIESTDYQAFSGKFKTGSWAWSLYYLYTG
                     VDDIPQRDIPSEEPTAYFSSHDLAIGFVLSRALNDRWFAGIGTKYIYERIQNTVTAWA
                     WDGGLYYVPNETWRAGFSFQNLGRSGKLIDERIRLPFQVRLSGGYTVYRQENRHRADL
                     MADVIKTYGASPRLHTGSEYVFQEKVCIRLGYVWGYASRNISAGVGLIFQRRMRLDYA
                     WTPFTNSLGSSHRISAGFDF"
     gene            complement(999..1481)
                     /locus_tag="PK754_16185"
     CDS             complement(999..1481)
                     /locus_tag="PK754_16185"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNB11093.1"
                     /translation="MADNAHKTLEDLIKSVEIFNGLDTRDINKVLKVATGKKFETDAV
                     VFREGDQGDCFYLIIEGKVCVSKSVSNDKIEEVAILSAGDYFGEMALFDGEPRSASVV
                     AIESTKLLEVKNSQFIKIIMSDENFSRKVLWAFCSTFAKRLRATNHLLSIFAKNQTPR
                     "
     gene            complement(1483..>2057)
                     /locus_tag="PK754_16190"
     CDS             complement(1483..>2057)
                     /locus_tag="PK754_16190"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016718973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNB11094.1"
                     /translation="PYSASKAGGDMMVRAYHKTYGVPTLITRASNNYGPYQYPEKLIP
                     LFVTNALDNIPVPLYGDGMNVRDWLYVEDHCSAVDYVLHHGVIGEVYNIGGGNERTNV
                     TITHTILKALNKPESLIKRVADRPGHDRRYAVDTSKVRRLGWKPAYNFEEAIVETIQW
                     YVKNESWWRAIKEKQKAFQEFMKKNYEDRK"
BASE COUNT          482 a          463 c          523 g          589 t
ORIGIN      
        1 accaaaaaac acggtacggg cttgggtttg ccgattgtca aatccattgt cgaagcgcat
       61 cgcggaaaag ttcagatcga aacccgaatc ggcagcggta caacatttat ttttacattt
      121 cccaaataaa atgacagtgc gtttaatatt atttttttcg ctgacgattt cacttcatgc
      181 cggatccggc gagacgggat ttgcttttct taagatcaat gccagcgccc gttcctcggc
      241 gatgggcgaa gtgagcacgt acgacggtca ggatcctatg ggggttttgg taaatccggc
      301 caatctttcc gtgacaccat cccatagtgt ggccttcacg tataaccggt ggattgaaag
      361 caccgattat caggcgtttt cggggaaatt taaaaccgga tcttgggcgt ggtcattgta
      421 ttacctgtac acaggtgtgg atgatattcc tcagcgcgat ataccctcgg aggaaccgac
      481 ggcgtatttt tcttcgcacg atttggcgat cggttttgtg ttgtcccgcg ctctcaatga
      541 tcgttggttc gccggtatcg gtacgaaata tatttatgaa cgcatacaaa atacggttac
      601 ggcctgggcg tgggacgggg gattgtatta tgtgcccaat gaaacatggc gcgctggttt
      661 tagtttccaa aatcttggac gttcgggcaa gttaatcgat gaacgcataa gactcccgtt
      721 tcaagtgcga ttatcgggtg gttatacggt gtatcgtcag gaaaaccggc atcgcgcgga
      781 tctcatggcc gatgtgatca aaacctacgg tgcatcgcct cgattgcata cgggaagtga
      841 atacgtgttt caggaaaagg tatgtattcg gttggggtac gtttggggtt atgcttcgcg
      901 caatatcagc gccggtgttg gtttgatctt tcaacgtcgt atgcgtttgg attatgcttg
      961 gacgcccttt accaatagtc tcgggtcatc acaccgtatc agcgcggggt ttgattttta
     1021 gcgaagatgc tcagcagatg gttggttgca cgcaagcgtt tggcgaaagt cgaacaaaaa
     1081 gcccacagga ctttgcgtga aaaattttca tcgctcatga tgattttgat aaactgactg
     1141 tttttaactt ccagaagttt ggtggactcg atcgcaacta cggacgcgga acgcggttcc
     1201 ccgtcaaaaa gcgccatctc accgaaataa tcaccggcgc tcagaatggc gacttcctca
     1261 attttatcgt tactcacgct tttggaaaca cacacttttc cttcgatgat caggtaaaaa
     1321 caatcacctt gatcgccttc tcgaaacacc accgcatcgg tttcaaattt tttcccggta
     1381 gcgactttta gaactttgtt gatgtcgcgg gtatccaatc cgttgaaaat ttcgacactc
     1441 ttgatgaggt cttccagtgt tttgtgtgcg ttgtcggcca tattatttcc gatcttcgta
     1501 gttctttttc atgaattcct gaaatgcttt ttgtttttct ttgatagcgc gccaccagga
     1561 ttcatttttc acataccatt ggatggtttc gacgatggct tcttcaaaat tgtaagcagg
     1621 tttccaaccc agacgacgca ctttggaggt atccacggca taacggcgat catggccggg
     1681 gcggtcggct acgcgtttga tcagagattc gggtttattc agagctttta ggatcgtgtg
     1741 tgttatggtg acgttggtgc gttcgttacc gccgccgata ttatagactt cgccgatcac
     1801 gccgtgatga agcacataat ccacggccga gcaatggtct tcgacatata accagtcgcg
     1861 cacattcatg ccgtcgccat agagaggaac aggaatgtta tccaaagcat tggttacaaa
     1921 aagcggaatc agtttttccg gatattgata ggggccgtag ttattggatg cgcgggtgat
     1981 caaagtcggg acgccgtacg ttttatgata agcgcgcacc atcatgtcgc cgccggcttt
     2041 actggcggaa tacgggc
//
LOCUS       DAOQNQ010000450         2019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_37248_length_2019_cov_4.830448, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000450 DAOQNQ010000000
VERSION     DAOQNQ010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37248_length_2019_cov_4.830448"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="PK754_16195"
     CDS             <1..1088
                     /locus_tag="PK754_16195"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNB11095.1"
                     /translation="AAGCIGGGLLFVLLNKIVNDNGGFLRKAATTMQHLNKLKLRSYQ
                     SFYRRLSKVPLFQILPPEELTALVRTVKTHRFKKDECILRQGDPGESMFVIQSGDVLI
                     TDPKQNNRTIATLRASDVFGEMALITGEPRTASATAQTDVLAWEIHKKDFDFLIRQSP
                     VLAAAMHSLVETRLADLKEHGPVSDEHAEAWYDDATHHLEAHMQPPTAQEVKQVSESH
                     GGAPLAIWLGIFLDGIPESLVIGSSMLHASISFSLLAGLFLSNFPEALSSSVGMRQQK
                     YSGQKIFWMWMSLMIATGIGAYFGNLFFAEAPLFLFALVEGLAAGAMLTMIAETMLPE
                     AFHKGGSVTGLSTLFGFLAAIFFKTLE"
     gene            1222..>2019
                     /locus_tag="PK754_16200"
     CDS             1222..>2019
                     /locus_tag="PK754_16200"
                     /inference="COORDINATES: protein motif:HMM:NF028457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5117 domain-containing protein"
                     /protein_id="HNB11096.1"
                     /translation="MVRTFFLFIFAGLSLWGQTSSLANKTATMKKYDGYIPYYWDEKS
                     GKIFLEVERWDNDFIYIVGLSHGVGSNDIGLDRGQIGGSRLVRFTRVGPKVLLIESNT
                     YYRANSGDPRERQAVRESFAESILWGFTVDAEEGRRVLVDATSFFMRDAHDVAGTLKS
                     AEQGNYQVDASRSAMYMERTKNFPRNSEFDAVLTFTGGPAGEWLRSVTPSPDAVTVHQ
                     HHSFVQLPDPGYQPRLHDPRAGMIWIEFHDYATPIAEPIKKRWITRHR"
BASE COUNT          469 a          474 c          527 g          549 t
ORIGIN      
        1 tggccgccgg ttgcatcggt ggcgggctac tttttgttct gctgaataaa atcgtcaatg
       61 acaacggtgg ttttctgcgt aaagcggcga cgacgatgca gcatttgaat aaacttaaac
      121 ttcggagtta tcaatcgttt tatcgccgtt tgagcaaggt tccgctgttt cagattcttc
      181 cgcccgaaga actcaccgcg ctggtacgta ccgtcaaaac acatcgtttc aaaaaagacg
      241 aatgtattct tcgccagggt gatcccggcg aaagcatgtt tgtcattcaa tcaggcgacg
      301 tgctcatcac cgatcctaag caaaataatc gcaccattgc gacattgcgt gcgtcggatg
      361 tgtttggtga aatggcatta atcaccggtg aaccgcggac ggcttccgca acggcccaga
      421 cggatgtgtt ggcgtgggag attcacaaaa aagattttga ttttttaata cgccagtcac
      481 ctgtgctcgc tgcggcgatg cacagtttgg tagaaacgcg tttagccgat ctgaaagaac
      541 acggtccggt cagtgatgaa catgccgaag cgtggtatga tgatgccacg catcatttgg
      601 aggcgcatat gcaacctccg acggcacagg aagtcaaaca agtttcggaa tcgcatggag
      661 gtgcgccgct cgcgatatgg ctcggtatct ttttggacgg cattccggaa tcgcttgtca
      721 tcggttctag tatgctccat gcttcgatca gtttttcgct tttggccgga ttatttttat
      781 ccaatttccc ggaagcattg tccagttccg tcggtatgcg tcaacaaaag tacagcgggc
      841 aaaaaatctt ttggatgtgg atgtcgctta tgattgcgac agggattggc gcgtatttcg
      901 gcaatttgtt ttttgccgaa gcgccgctat ttctttttgc gcttgtcgaa ggattggccg
      961 ccggtgcgat gcttactatg attgcagaaa ccatgttacc ggaggcgttt cacaaaggcg
     1021 gctccgtgac cgggctatcc acactgtttg gctttttagc agccatcttc ttcaaaactt
     1081 tggaatgaag agagacctcg cttttcggtg caactttttc ggcacagact tcgtaagcga
     1141 atggtaatcg tctaatcagt ctagccagtc atatatattt ctacttcatt ttcggatcca
     1201 aaaaaatacc ggagaattca aatggtgcgc accttttttc tgttcatttt tgccggactt
     1261 tcactgtggg gacaaacgtc ctctctcgcg aacaaaaccg cgacgatgaa aaaatacgac
     1321 ggatacattc cgtattattg ggatgaaaaa tccggaaaaa tttttttgga ggtcgaacga
     1381 tgggataatg atttcattta catcgtaggg ctttcgcacg gcgttggctc caatgatatc
     1441 ggtttggatc gcgggcagat cggcggtagt cgtttggttc gttttacccg cgtcggtccc
     1501 aaagtgcttt tgatcgaatc caatacgtat tatcgcgcca attcaggtga cccacgtgaa
     1561 cggcaggccg tacgcgaatc atttgccgaa tcaattttgt ggggatttac ggtcgacgcc
     1621 gaagaagggc gccgcgtgct ggtggatgcc acgtcttttt ttatgcggga cgcgcacgat
     1681 gtggccggta cgctcaaaag cgcggaacaa ggaaattatc aagtggatgc ttcgcgcagt
     1741 gcgatgtata tggagcgaac caaaaacttt cctcgcaatt cggagtttga tgccgtattg
     1801 acgtttacag gcggcccggc cggcgaatgg ttgcgctccg tgacaccttc gcccgatgcg
     1861 gtgactgttc atcagcatca ttcatttgtg cagttaccgg atccgggtta tcaaccgcgt
     1921 ttgcatgatc cacgcgccgg tatgatttgg atcgaatttc atgactatgc gacgccgatc
     1981 gcggagccga tcaaaaaacg ctggatcacg cgccaccgt
//
LOCUS       DAOQNQ010000451         2009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_37593_length_2009_cov_5.990276, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000451 DAOQNQ010000000
VERSION     DAOQNQ010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37593_length_2009_cov_5.990276"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PK754_16205"
     CDS             <1..794
                     /locus_tag="PK754_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11097.1"
                     /translation="ANKPNAYYGRALSMGKKFALASFSVRGTIYHRDVKSSSSTQTMG
                     NVEVTTTTTTITTLEFPQLPDEFWDKLLDNMYSDFVTALKNEHQIELIPVESVTKASG
                     YAAMEPIEDKNTQVKIVRSYKNTKNLLPTSLTSILKSVSSTFASDRPDARLMRELGVD
                     GLISATLDLQISTDENNHVVLNPVFTYQLLGPPNGYLVGTQYVTGTVLGKGAPFNAEE
                     VKSNPNSLNGVVRKEDMMAAFRKSIIELKKLEEAEGYTKIWNLQK"
     gene            complement(920..1669)
                     /locus_tag="PK754_16210"
     CDS             complement(920..1669)
                     /locus_tag="PK754_16210"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNB11098.1"
                     /translation="MIDVTQHDGIAILQMQHGKANALDIEFCHALAHAVDNLRHVEVH
                     GLILTGTGTMFSAGVDLPRLIKSGPEYIPEFLAALHHTFETLFTFPKPMIAAINGHAI
                     AGGCVLAACADYRIMAGGRIGVSELRVGVPFPVSALEIMRSVVPQKFLSEVLYDAQNY
                     TPDDAKTVGLIDAITGPDELMSEAVKKVKSWSAIPATAFAITKRQLQAPYLDRIHHNA
                     IDTYVVNLWKEPATLNAIGNFVASTIKKTQP"
     gene            complement(1688..>2009)
                     /locus_tag="PK754_16215"
     CDS             complement(1688..>2009)
                     /locus_tag="PK754_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB11099.1"
                     /translation="TATFKTGAVVFTPVITATPPKTADAVKHYNDAMSEFMEASKNFT
                     DAQLNKNYPFEMGGQVVWNPTGMELISGYICHEIHHRAQIGVVLRLVGAKVPGMYGPT
                     ADDM"
BASE COUNT          548 a          450 c          485 g          526 t
ORIGIN      
        1 ccgccaataa acccaatgcc tattacgggc gcgctttatc catgggcaaa aaatttgcgt
       61 tagcctcctt ttctgtacgc ggaactattt atcatcgcga tgtgaaatct tctagttcaa
      121 cgcaaacgat gggtaatgtc gaagtcacga caaccactac gacaattacg actttggaat
      181 ttccgcagtt accggacgaa ttttgggata aactgctgga taatatgtac agtgattttg
      241 taacggcact gaaaaacgaa catcagatcg agttgattcc agtcgaaagc gttacgaaag
      301 cttccggcta tgcggcgatg gagccgatcg aagataaaaa cacgcaggtc aaaatagttc
      361 gcagttataa aaataccaaa aaccttcttc ctacgtcgct aacttcgata ctcaagtcgg
      421 tttcatccac atttgccagc gaccggccgg atgcacgtct gatgcgcgaa ttgggggtgg
      481 atggtttgat cagtgcgacg ttggatttgc agatttcgac agatgaaaac aatcacgtcg
      541 tacttaaccc ggtttttacg tatcagttgc tcggaccgcc caatggttat ctcgtcggca
      601 cacaatatgt taccggtacg gtgctcggca aaggtgcccc gtttaatgcc gaagaagtca
      661 aatccaatcc caatagtctc aatggggtag tgcgcaaaga agatatgatg gccgcttttc
      721 gcaaatccat catcgaatta aaaaaactgg aagaagcgga gggttatacc aaaatttgga
      781 atttacaaaa ataaatttgc agcggtttaa ccgataaaaa aatagacctc aaaatagttt
      841 ttctttaaaa caagtcgtga tataaaatga aatcacagct gaaaaagaaa actgttttga
      901 ggtctatttg taagatattt taaggttggg ttttctttat ggtgcttgct acgaagttac
      961 cgattgcgtt aagcgtggcc ggttctttcc agagatttac gacataagta tcaatcgcat
     1021 tgtggtgaat gcgatcaagg tacggggctt gtagttgtcg tttagtaatt gcaaaagcgg
     1081 tagccggaat ggccgaccag gattttactt ttttgacggc ttcggacatg agttcgtcag
     1141 gaccggtgat cgcatctatc aagccgacgg tttttgcgtc atccggagta tagttttggg
     1201 cgtcgtacaa tacttcgctc aaaaattttt gaggtacaac ggatcgcatg atctccagag
     1261 ccgataccgg aaatggaacc ccgacgcgca attccgaaac gccgatacga cctccggcca
     1321 tgatgcgata atccgcacag gcggccaata cgcagccacc ggcaatggcg tgaccattga
     1381 tagcggcgat catcggtttt ggaaaagtaa aaagtgtttc gaaggtgtga tgaagcgccg
     1441 caagaaactc ggggatatac tccggcccgg atttgattag gcgaggtaaa tcgacacccg
     1501 cagaaaacat cgtgcctgtt ccggtgagga taaggccatg cacctccaca tgccgcaggt
     1561 tatccaccgc gtgtgcaagc gcatggcaaa attcgatatc cagcgcattg gctttgccgt
     1621 gctgcatttg cagtatggcg attccgtcgt gttgcgtaac atcgatcata tccgtatcgt
     1681 ccttttttta catatcgtcc gctgtcggcc cgtacatgcc gggtacttta gcgccgacta
     1741 aacgcagcac gacaccgatc tgtgcacggt gatgtatttc gtgacaaata taaccggaaa
     1801 tcaattccat acccgtggga ttccaaacca cttggccgcc catttcaaaa ggataatttt
     1861 tattcagttg tgcatccgta aagtttttgc tcgcttccat aaattcgctc atcgcgtcat
     1921 tatagtgttt gacggcatcg gcggttttag gcggtgtcgc ggtaatgacg ggtgtaaaca
     1981 caacagcgcc ggttttgaag gtagcggta
//
LOCUS       DAOQNQ010000452         2005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_37765_length_2005_cov_4.716410, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000452 DAOQNQ010000000
VERSION     DAOQNQ010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37765_length_2005_cov_4.716410"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PK754_16220"
     CDS             <1..351
                     /locus_tag="PK754_16220"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11100.1"
                     /translation="LGYGSWVYPERDGTAKIGVTDLFVKAMENLTAIELTPMLSDIYQ
                     GNSCAQMRDARGLMHAVMCPLSGQVIQVNEELTEAPSQIEKDPFFEGWFYRIIPTDLE
                     YELPKLVSCSSDRL"
     gene            400..1563
                     /locus_tag="PK754_16225"
     CDS             400..1563
                     /locus_tag="PK754_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB11101.1"
                     /translation="MPLLIVALVLFVILDIAIRMIVRRMQDEKQRKLREEALAVSLRM
                     DYSREAKTLKRAEVPAPKARILCVDDEPVILDSFRKILVIDGYNVDTVETGQEALGLV
                     QAHHYDFVFTDLKMPAMDGVDVVKSVKEMRPDMDVIVITGYATVESAVECMKFGAMDY
                     IQKPFTEEELLNFTRKTLIKRQDRIQKQLMPKVHITHVHGAEQFMTKEFTIPGGMFIA
                     RNHCWVSMETEGDVKVGMDDFAKKLIGKIDGFEFPNRGMNIQVGQPLFSVKQGNRIIH
                     FKSPVSGKVTEINHALVKDPDRILVTPYDNNWICIIDPDRVDDDIRHLKIGKSAVQFY
                     QEDLQNYQNEVKTMVKGNGHGKPGDIYTGEMQQLEDKDWALVTGHFFERELVS"
     gene            complement(1668..>2005)
                     /locus_tag="PK754_16230"
     CDS             complement(1668..>2005)
                     /locus_tag="PK754_16230"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HNB11102.1"
                     /translation="MAAGALTECMGGNPDQVLMAAEIAMEHHLGLTCDPIGGLVQIPC
                     IERNTMGAIKAITASQIALNSDPKMAKVSLDAVIKTMWQTAKDMDNRYKETSEGGLAT
                     NVPVNLPEC"
BASE COUNT          555 a          417 c          499 g          534 t
ORIGIN      
        1 cttggttacg gcagttgggt ctatcctgag cgcgacggca cggcaaaaat cggtgtaacc
       61 gatttatttg taaaggcaat ggaaaacctt acggcgatcg aattgacgcc gatgctgtcc
      121 gatatttatc aaggtaattc ctgcgcacag atgcgtgatg cgaggggatt gatgcatgct
      181 gtgatgtgcc cgctcagcgg gcaggttatt caggtcaatg aagaattgac cgaggcaccc
      241 agtcaaattg aaaaagatcc gtttttcgag ggatggtttt accgaatcat tcctacggat
      301 ttggaatacg aactgccgaa attggtatcc tgtagttcgg atcgtttata aaaggatacg
      361 attttcatta aaagcaatca cctacgaatg gaggcttcta tgccgttact cattgttgct
      421 ttagttcttt tcgtcatact cgatattgcg atacggatga tcgtacgtcg aatgcaagac
      481 gaaaagcagc gcaaactccg ggaagaagca cttgccgtga gtctcagaat ggattacagc
      541 cgagaggcaa aaaccctcaa gcgggccgaa gtgcctgcac ccaaagcgcg gatactctgc
      601 gtggatgacg aacccgttat tttagacagt tttcgcaaaa tactcgttat tgacggatac
      661 aatgtggata ccgtcgaaac gggacaggaa gcgctcggac tggtacaagc ccatcattat
      721 gattttgttt tcaccgatct caaaatgccg gctatggatg gtgtggacgt agtcaaatcc
      781 gtcaaagaaa tgcgccctga tatggatgtt attgtcatta caggctacgc tacggtagag
      841 tcggcggtcg aatgtatgaa atttggtgca atggattata tccagaagcc tttcacggaa
      901 gaggagttac tcaattttac gagaaaaacg cttatcaaac gtcaagatcg gattcaaaag
      961 caattgatgc ccaaagtgca tatcacgcac gtgcatgggg ccgaacaatt tatgaccaaa
     1021 gagtttacga ttcccggcgg tatgttcatc gctcgtaatc attgctgggt cagtatggaa
     1081 accgaaggcg atgtcaaagt tggtatggat gattttgcga aaaaactgat aggaaaaatt
     1141 gacggattcg aatttccaaa tcgcggaatg aatattcaag ttggtcaacc gctatttagt
     1201 gttaagcagg gcaaccgcat tattcatttc aagtcgcccg tcagcggtaa agtgacagag
     1261 atcaatcatg cactggtcaa agatccggac cgtattttgg taacgcctta tgacaataac
     1321 tggatttgca ttattgatcc ggatcgcgtg gacgatgaca ttcgccatct caaaatcgga
     1381 aaatcagcgg tgcagttcta tcaggaagat ttacagaatt atcagaatga agtaaaaacg
     1441 atggtaaagg gtaatggcca cggcaaaccg ggtgatatat ataccgggga aatgcagcaa
     1501 ctcgaagata aggattgggc tttggttacc ggacatttct ttgagcgcga attggtttcc
     1561 tgatatttca ggtttgccgg ccaatacccg gtaggttgat ttctaagtta atgaaaaacc
     1621 cctctgcagg atgtggaggg gtttttttac atatataata ccaactatca gcattccggc
     1681 aaattgacgg ggacgttggt agctaatccg ccttcggatg tttctttata acggttatcc
     1741 atatctttag cagtttgcca catcgtcttt ataactgcat ccagagaaac tttagccatt
     1801 ttcggatcgc tattgagggc tatttgacta gctgtaatgg ctttgatcgc gcccatcgta
     1861 ttgcgttcga tgcatggtat ttgcactaag cctccgatcg gatcacaggt aagaccaagg
     1921 tggtgttcca tcgcgatttc ggccgccatc aatacctgat caggattacc gcccatacat
     1981 tcggtgaggg cgcccgccgc catgg
//
LOCUS       DAOQNQ010000453         2004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_37810_length_2004_cov_3.628014, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000453 DAOQNQ010000000
VERSION     DAOQNQ010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37810_length_2004_cov_3.628014"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2004
                     /locus_tag="PK754_16235"
     CDS             <1..>2004
                     /locus_tag="PK754_16235"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNB11103.1"
                     /translation="PKNASTHKVQFVFADSLTGWAIGSIKTGGALKDSLNPKALFITG
                     ILKTYDGGLNWELQTVLGVEENGWEVRATASLNRTHVWAILSKPNSDQNVLLRTTDGR
                     RWTMQSLKLADGDPVQICFSDPANGFMILREFMGSTILRTTDGGATWATFEPGLGSDY
                     RGVAFDGTSWYVLAQSRTKPEVVTVLRSDDNGNILIEAETFNPESGDHILEGFGIRSH
                     KNKISIMLRDIDSKTRETSRWFWIQSGDRFVSHTSQAFQYEKEFKSPSDIQNFSIDHS
                     IYVLDRSEEYEPGLPDAKIRYSLIRSVDEGKSWTSIPEFRFPVLALQAVGDDVLISAN
                     DGNLYKFNYITGIYQTASVDLKKAFLNPPQVGTESVAENGENIFDLGHDEFEKESVYE
                     SKWESANDSAFAESFSSVRDANKYKPDAKAEFSVGIDSITSIGVVLHRRTRMGSKHQA
                     INLINEELQKGEIRLRKYKPITFKGTVRSTGTRFPVKYSWSSSISGELSDQIEFTTSP
                     RQLPPGVHYVFFKAQDNLGTWSNPVVVKVIVDDFPKYKFPFHGNWVLGGGGSHYNIGH
                     HIRTVPYGIDVNNIEQGYGGDADIGFPIRASTDGIVSLAGYIKGYGRTVKIDYMYGGK
                     KYTTLVSHLSTISVEVGEYVKQGQEVGTVGSTGRSSAPHIHWEL"
BASE COUNT          565 a          417 c          482 g          540 t
ORIGIN      
        1 ccaaaaaacg cttctacgca taaagtacaa tttgtttttg cggatagcct tacgggttgg
       61 gcgatcggat ccattaaaac cggtggtgca ttaaaagaca gcttaaatcc taaggcgctt
      121 ttcataacag gtattttaaa aacatatgac gggggattga attgggaatt gcaaaccgtc
      181 ctcggcgttg aagaaaacgg gtgggaagtg cgtgcaacgg ccagcctcaa tagaacgcat
      241 gtttgggcga ttttaagtaa acccaattca gatcaaaatg tattattacg cacgaccgac
      301 ggtcgccgct ggaccatgca atcattgaag cttgccgatg gcgatccggt tcaaatttgc
      361 ttttccgacc cggctaacgg gtttatgatt ctacgcgaat ttatgggcag caccatattg
      421 cgcacgaccg atggcggcgc tacttgggcg acatttgaac cggggcttgg atccgattat
      481 cgcggcgtgg cgttcgacgg tacatcatgg tatgtattag cccaatctcg tactaaacct
      541 gaagtggtta ctgttttacg cagtgatgat aatggtaata ttttgatcga agccgaaact
      601 tttaatcctg aatcgggcga tcacatatta gaaggattcg gcattcgctc acacaagaat
      661 aaaatctcca taatgctgcg cgatattgat tccaaaacgc gtgagacttc ccggtggttt
      721 tggatacagt ccggagatcg ttttgtatcc catacctcac aagcgtttca atatgaaaag
      781 gaattcaaat cgccttcaga tattcaaaac ttctcaatag atcactcgat ttatgtcctt
      841 gatcgctcgg aagagtatga accgggattg ccggacgcta aaataagata tagtcttatc
      901 cgttcggttg atgaaggaaa aagttggacg tccattcctg agtttcgttt tccggtacta
      961 gcactccagg ctgtcggcga cgatgttctt attagcgcca atgacggcaa tctgtataag
     1021 tttaattaca ttacaggtat ttatcagacg gcatccgttg atctgaaaaa agcttttttg
     1081 aatccgcctc aggttggtac agaaagtgtc gccgagaatg gagaaaatat atttgatctc
     1141 ggccatgatg agtttgaaaa agaatcggtt tatgaaagca aatgggaaag cgcaaatgac
     1201 agtgcattcg ctgaaagttt tagtagcgta cgggatgcta acaaatacaa accggatgct
     1261 aaagccgagt tttctgtcgg tattgattcg ataacatcta tcggcgtggt cctacatcgg
     1321 cgcacacgta tgggatctaa acatcaggcg atcaacctga tcaacgaaga acttcaaaaa
     1381 ggtgaaatac gccttcgtaa atataaacct atcacattca aagggacggt tcgcagcacg
     1441 ggtacccgtt ttcctgtcaa atacagttgg agttccagta ttagcggcga actcagcgat
     1501 cagatcgagt ttactaccag tccgcgtcaa ttgccgcccg gtgttcatta tgtatttttt
     1561 aaggcccagg ataatttagg cacatggtcc aatccggtag tggtaaaagt tatcgtagat
     1621 gattttccta aatacaaatt tcctttccac ggaaattggg tgctcggtgg cggcggcagt
     1681 cattataata tcggtcatca tattcgtacg gttccgtatg gtattgacgt aaataatatc
     1741 gaacagggtt acggcgggga cgccgacatc ggttttccta ttcgcgcatc aacggacggt
     1801 attgtttctt tggccggcta catcaaaggt tatggccgaa ccgtaaaaat agattatatg
     1861 tacggcggga aaaaatatac gactttagtt tcacatctat cgaccatcag cgtggaagtc
     1921 ggtgaatacg ttaaacaagg acaagaagtc ggaacggtag gaagcaccgg ccgatccagt
     1981 gcgccgcata ttcattggga gttg
//
LOCUS       DAOQNQ010000454         2002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_37870_length_2002_cov_5.560349, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000454 DAOQNQ010000000
VERSION     DAOQNQ010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37870_length_2002_cov_5.560349"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PK754_16240"
     CDS             complement(<1..372)
                     /locus_tag="PK754_16240"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNB11104.1"
                     /translation="MDFFQAIVVGIIQGITEFLPISSTAHVRIVPALLGWPDPGAAVT
                     AVTQLGTLMAVLIFFRNDIIRLIKAAFFSVTRWSQKAQWDAELRQDVRLAWFIALGTL
                     PLGILGIIFKDTIEIDFRSLTI"
     gene            complement(451..1035)
                     /locus_tag="PK754_16245"
     CDS             complement(451..1035)
                     /locus_tag="PK754_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HNB11105.1"
                     /translation="MFINWALQEINLKIVYYGPGMSGKTTNLEYIHSKMDPSLAGELV
                     SLKTKEDRTIFFDFLQLEVGRIKGKKPKFNLYTVPGQVYYASSRKVILNGVDGIVFVA
                     DSQKHRMDGNIETLLDLENNLREDGHSLDSFPWVIQYNKRDLPDVDTVENLQKRLNYF
                     NVPYYEAVGIKGVGVFDTLKAIINKVIANVQTQL"
     gene            complement(1086..1601)
                     /locus_tag="PK754_16250"
     CDS             complement(1086..1601)
                     /locus_tag="PK754_16250"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNB11106.1"
                     /translation="MLQTKKLKDGSKQIILNGKQFEGVTKVLSELASKTKASAILFAD
                     MSGQLISQRGNTDKMNTTVLSALAASDFAATAEMAKLVGEDAKFKLLFHEGEKRNVYL
                     SNVGDNFFLVVVFDTSVTLGLIRIYTKKAIQNLIEIFEDSSNSSDDSAKDLIDSEFGS
                     LLGDALDESFK"
     gene            complement(1738..>2002)
                     /locus_tag="PK754_16255"
     CDS             complement(1738..>2002)
                     /locus_tag="PK754_16255"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNB11107.1"
                     /translation="RATALLVGGVLALAVVFGMIVALYLSNALTKQLLYLTESAEQIS
                     KGDLESIVRVEATDEIGDLADAIERLRESLKAAIERLRKKKAG"
BASE COUNT          554 a          480 c          422 g          546 t
ORIGIN      
        1 gatggtgagg ctacgaaaat cgatttcaat cgtatctttg aaaataattc ccagtattcc
       61 gagcggcaag gttccaagtg caataaacca agccaatcgt acgtcctgtc gtaactcggc
      121 atcccattgc gctttctgac tccatcgtgt cacggaaaaa aaagccgctt tgataagacg
      181 gataatatcg tttctaaaaa agatcagcac cgccatcagc gtcccaagct gtgtcaccgc
      241 cgtcacggcg gcgccgggat ctggccaacc caaaagcgcc ggcacgatgc gaacatgcgc
      301 cgtactgctg atcggcaaaa attcagtaat cccttgtatg ataccgacca ctatggcttg
      361 aaaaaaatcc atacgtttcc tgaagtttaa agtaacaata aaaaagccct acaccaatga
      421 tgtagggctt ttttccgcat atcagaaaaa ttataactgt gtttgaacgt tggcaatcac
      481 tttattgatg atcgctttga gcgtgtcaaa aacacctacg cctttgatac cgacggcttc
      541 ataatacggt acattaaaat aattgaggcg tttttgaaga ttttccaccg tatcaacatc
      601 cggaagatcg cgcttattgt attggatcac ccaaggaaac gaatccagcg agtgaccgtc
      661 ttcacgcaga ttgttttcca gatcaagcaa tgtttcaata ttgccgtcca tacgatgctt
      721 ttgcgaatcg gcaacgaaaa cgataccgtc cacgccgttg aggataactt tacggctgga
      781 ggcgtaataa acctgaccgg gaacggtgta taaattaaat ttcggttttt tacctttgat
      841 acgaccgact tcaagttgta aaaagtcgaa aaatatcgta cgatcttctt tggttttaag
      901 cgatacgagt tcgccggcca gcgagggatc cattttggaa tgaatgtatt ccaaattggt
      961 cgtcttgccg ctcataccgg ggccgtagta cactatcttc agattgatct cttgtaatgc
     1021 ccagttgata aacattcgta tcctctctaa ggttttatgg ttgaatcagc agaccggata
     1081 atccgttatt taaacgattc gtcgagcgca tccccgagaa gcgaaccgaa ttcggagtca
     1141 atcaggtctt ttgcggaatc atcgctggaa ttggaagaat cttcaaaaat ttcaatcaga
     1201 ttctggatgg cttttttcgt ataaatacga atgaggccga gcgttacgct ggtatcaaac
     1261 acgacgacga ggaaaaaatt gtcgccgaca ttggaaagat aaacattgcg tttttcacct
     1321 tcgtgaaaca acaatttgaa ttttgcgtct tcgcccacga gtttggccat ttccgccgtc
     1381 gctgcaaaat ccgatgcagc taacgctgaa agcactgtcg tgttcatttt gtccgtattg
     1441 ccgcgctggc tgatgagctg gccgctcata tccgcaaaaa gaatcgccga agctttcgtt
     1501 ttcgacgcca gctcagacaa aactttggta acgccctcaa attgtttccc gttgagaata
     1561 atttgcttgc taccgtcttt caattttttc gtctgcaaca tagcggttac cctttgctct
     1621 ggttatggta atcttaaaag aataatcaaa attggatctt gataagcgga cgtgcatccg
     1681 tattacggat taacaaccgt aacctatgga attttcgtcc tttagtcaaa taatttttta
     1741 accggccttt ttctttcgca aacgttcgat agccgctttc aacgattcac gcaaacgctc
     1801 aatcgcatcc gccaaatcac cgatttcatc ggtcgcttcg acgcgcacga tggattccag
     1861 atcgccttta ctgatctgct cggccgattc cgtcagatac aatagctgtt tggtaagcgc
     1921 gttcgaaaga tacaacgcga cgatcatacc gaaaaccaca gccagtgcga gtacgccgcc
     1981 cactaaaagc gccgtagcac gg
//
LOCUS       DAOQNQ010000455         1975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_38905_length_1975_cov_5.538542, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000455 DAOQNQ010000000
VERSION     DAOQNQ010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38905_length_1975_cov_5.538542"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PK754_16260"
     CDS             <1..294
                     /locus_tag="PK754_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN reductase"
                     /protein_id="HNB11108.1"
                     /translation="VGSGELVYKPVAVINAAPHATYAYASIIETLRVMTADIDPVASK
                     VLPSAGQKLSDDEIYTHKEISNALRSTLVSLAQTIRSRDAATSSEKNASWNLF"
     gene            313..861
                     /locus_tag="PK754_16265"
     CDS             313..861
                     /locus_tag="PK754_16265"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HNB11109.1"
                     /translation="MSTYIALLRGINVSGQKLIKMNDLKSYFVSYGYKNVQTYIQSGN
                     VVFESAKQESEKLSKKVGEQLAVSLGYIVPVIARSLNEWVIVNENNPYAKKKLRPDER
                     VYVTLLAIKPKPDLAQSLNALSDTKDEFQLNGKTVYILCRKGYGNSKFSNNFIEKKLD
                     TQATTRNLATMQKLQMIAEGLI"
     gene            965..>1975
                     /gene="gap"
                     /locus_tag="PK754_16270"
     CDS             965..>1975
                     /gene="gap"
                     /locus_tag="PK754_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013243364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNB11110.1"
                     /translation="MAIKVGINGFGRIGRLVFNALVDKGLLGKEIDVVAVVDVSTDAD
                     YFAYQLKYDSIHGKFKGTINVAKSKPDLDHNDILIVNGDRVQCVAATKDPSQLPWKDL
                     GVDYVIESTGLFTDSEKAKGHLTAGAKKVIISAPGKGDVKTIVMGVNEQEYDASKHHI
                     ISNASCTTNCLAPLVHVLLKEGIGIETGLMTTIHSYTATQKTVDGPSKKDWRGGRAAA
                     INIIPSTTGAAKAVGEVLPATKGKLTGMSFRVPTADVSVVDLTFRATRDTSIEEIDGL
                     MKKASETYLKNILGYCNEELVSTDFIHDERSSIYDSLATTQNNLKGEKRFFKVVSWYD
                     NEW"
BASE COUNT          618 a          405 c          437 g          515 t
ORIGIN      
        1 gtgggaagcg gtgaattggt atacaaaccg gtcgctgtaa taaatgccgc accgcatgcc
       61 acgtatgcat acgcttcaat catcgaaaca ctacgggtga tgacggcaga tattgatccc
      121 gtagcatcca aagtattgcc atcagcaggc caaaaactgt ccgacgatga aatttatacg
      181 cataaagaaa tttccaacgc attacgcagt acgttggttt cattagctca gacgattcgc
      241 tctcgtgatg cggcaacatc atccgaaaaa aacgcttcat ggaatttatt ctaaaactga
      301 gcaaaagcca ttatgtcaac atacatcgca ttgcttcgtg gtatcaatgt gtcgggacaa
      361 aaactgatca aaatgaatga tttgaaaagt tattttgttt cgtacggtta taagaatgta
      421 caaacgtata ttcaaagcgg aaatgtagtc tttgagagtg ccaaacaaga gtccgagaaa
      481 ttgagcaaaa aagtggggga acaattagcc gtttccttgg gctatattgt ccctgttatt
      541 gcgcgtagtt tgaacgaatg ggtaatcgta aatgaaaata atccttatgc aaagaaaaaa
      601 ctcaggcctg atgagcgcgt gtacgtgacg ttattagcta taaaaccaaa acccgattta
      661 gcacaatccc tgaacgcatt atcggataca aaggatgaat ttcaattgaa cgggaaaacg
      721 gtttatattc tgtgtcgaaa aggctacgga aactccaaat tctcgaacaa ctttattgaa
      781 aagaaattag acacccaagc cacaactcgg aatctggcga cgatgcaaaa actgcaaatg
      841 attgccgaag ggttgatatg acagcgtcgc aatccttatt ttcggttgac tttccatcat
      901 acaaatccta tattcgcccc agtttaacaa cttcataatt ttcaacgctt ttcgggagag
      961 gattatggct atcaaagtcg gtattaacgg attcggtcgc atcgggcgtt tagtatttaa
     1021 tgcccttgta gataaaggcc ttttgggtaa agaaatagac gtagttgctg tagtagatgt
     1081 cagtacagat gcggattatt tcgcctacca actcaagtat gattctattc acggaaaatt
     1141 taaaggcact atcaatgttg ccaaaagtaa accggatttg gatcacaatg acattctcat
     1201 tgtcaacggt gatcgggtac agtgtgttgc agcgaccaaa gatccgtcgc aactgccttg
     1261 gaaagatctg ggtgtggatt atgtgattga atccacaggt ttgtttacgg attcggaaaa
     1321 agcgaaaggc catttaacgg ccggagctaa gaaagtaatt atttcagcac ccggtaaagg
     1381 ggatgtcaaa accatcgtta tgggtgtcaa tgagcaagaa tacgatgctt ccaaacatca
     1441 catcatttcc aatgcatcct gtacgacgaa ctgccttgca ccgttggtac acgttttact
     1501 caaagaaggt atcggtatcg aaacgggtct gatgacgacg attcacagtt ataccgcgac
     1561 gcaaaaaacc gtagacggcc cttctaaaaa agactggcgc ggtggccgtg ctgcggcaat
     1621 aaatatcatt ccttcgacta ccggtgccgc taaagccgta ggggaagtgt tgcctgcgac
     1681 caaaggaaaa cttaccggta tgtctttccg cgtaccgacg gccgatgtat ccgtcgtgga
     1741 tcttactttt cgcgcaacgc gcgatacgtc cattgaagaa attgacggat taatgaaaaa
     1801 agcttcggaa acgtacttaa aaaacatact tggctattgc aacgaagagc ttgtctcgac
     1861 agattttatt catgatgaac gctcatccat ttatgattca ttagcaacca cgcagaacaa
     1921 cctcaaaggt gaaaaacgat tttttaaagt cgtttcttgg tacgataacg aatgg
//
LOCUS       DAOQNQ010000456         1948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_40063_length_1948_cov_4.263074, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000456 DAOQNQ010000000
VERSION     DAOQNQ010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40063_length_1948_cov_4.263074"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PK754_16275"
     CDS             complement(<1..451)
                     /locus_tag="PK754_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNB11111.1"
                     /translation="MPLIDGFGRSIDYLRVSVTDRCNFRCVYCMPHDGVQFMPREELL
                     TYDELERIIGVFVNLGIEKIRITGGEPTVRKDIIELVRRIGSLPLRSYAMTTNGYLLD
                     ELAEPLRAAGLQRFNISLDTMRRDRFQKIARFDGYEKTWRGIRKAADL"
     gene            610..1326
                     /locus_tag="PK754_16280"
     CDS             610..1326
                     /locus_tag="PK754_16280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB11112.1"
                     /translation="MKPTVILIVDDNPHISKLLTMNLEQSAFTILHAMDGEEGLAKIK
                     TDKPDIVISDVMMPKLDGITMCQQVRTGSDVPMVPFIFLTSMDSDITQKRGFRMGADQ
                     YLIKSEINRDTINGKVNEILKHIRKIEEIRSQNSDTFKSDLGELSFIETLQYLYLQKK
                     TGTLTIIRQFYPEGKIIVRNGEILSATVGDDKDEKALLTISGWKRGTVQFLSDDVSAI
                     TPTVKTSTENIILESCRLTA"
     gene            complement(1356..>1948)
                     /locus_tag="PK754_16285"
     CDS             complement(1356..>1948)
                     /locus_tag="PK754_16285"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014489.2"
                     /note="biotin-containing enzyme that catalyzes a two step
                     carboxylation of pyruvate to oxaloacetate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HNB11113.1"
                     /translation="PPVDFDKEFEAFKQQFDANLSITDFLSYKFYPKVFTDYFENQQK
                     WGQVSVIPSEIFFFGMKPNEEAIIEIAQGKSIIVRLLSVSQADKDGNRKVFFELNGQA
                     RTITVQDRSLNVKRVQNRKADASKPGELASPLPGMLSKVLVKPGDTIKKSQPLCIIEA
                     MKMETTVTAVQDGVVKEIILGVKTIVESGDLLMILG"
BASE COUNT          541 a          476 c          408 g          523 t
ORIGIN      
        1 ccaaatccgc ggccttccgg ataccgcgcc acgttttttc atatccgtca aatcgtgcta
       61 ttttttgaaa acgatcacgg cgcatcgtat ccaaactgat attaaagcgc tgtaatcccg
      121 cagcgcgcaa cggctcggcc agttcgtcca acaaataacc gttggttgtc atcgcgtacg
      181 atcgtaacgg caaactcccg atacgccgta ccaattcgat gatgtcttta cgtaccgtgg
      241 gttcaccgcc ggtgatgcga attttttcaa tacccagatt tacaaaaacg ccgatgatac
      301 gttcgagttc gtcgtaagtc agtaattcct cacgcggcat aaactgaacg ccatcgtgcg
      361 gcatacaata cacacaacga aaattgcatc ggtcggtgac ggacacgcga agataatcta
      421 tggaacggcc aaagccgtca ataagcggca tattcgcttc ccggtaaggt cttgcacgaa
      481 aagtgagagt attttgtctg aaatgcaagt gtgcatggcg ccaaaatttg cttgtttttg
      541 tgaacgactc cgtatagttt cccgcgggtt cgttcaccgc tttcattaaa attattattt
      601 atcaatttca tgaagccgac cgtcattctg atcgttgacg ataatccgca tatttccaaa
      661 ctcctgacga tgaatctgga gcaatcggcg tttaccattc ttcatgcgat ggacggcgaa
      721 gaaggtctcg ctaaaatcaa aaccgacaaa cccgacatcg tcatttccga tgtgatgatg
      781 cccaaacttg acggtatcac catgtgccag caagtccgga ccggttcgga tgtcccgatg
      841 gtgcctttta ttttccttac ttcgatggat tcggatatta cacagaaacg cggcttccgt
      901 atgggcgcag atcagtattt gatcaaatcg gaaatcaatc gcgatacgat caacggcaag
      961 gtcaatgaaa tcctcaaaca catccgcaaa atcgaagaaa tccgctcaca aaactccgat
     1021 actttcaaaa gcgatttggg tgaattgagt tttatcgaaa cgttgcagta cttatatctt
     1081 cagaaaaaaa cagggacgct tacgatcatt cggcaatttt atccggaagg aaagatcatt
     1141 gttcgtaacg gtgaaatttt aagtgcgacg gtcggtgatg acaaagatga aaaagcattg
     1201 cttacgattt ccggttggaa gcgcgggacc gttcagtttt tatccgatga cgtatcggcg
     1261 atcacaccca ccgtcaaaac atccacggaa aatattattc tcgaaagctg ccgactcacg
     1321 gcttaagtat ccgattcatt ttccgcaccc acaaatcacc ccaaaatcat cagcaaatcg
     1381 ccactttcca cgatagtctt tacccctaat atgatttctt tgactacacc atcttgaacg
     1441 gctgtaacgg tcgtctccat tttcatcgcc tcaatgatac atagcggttg tgattttttg
     1501 atcgtatccc cgggcttgac caaaacttta gacaacatgc cgggcaaagg cgaagccaat
     1561 tcacccggtt tggaggcatc ggcttttctg ttttgtacgc gcttcacatt aagagaacga
     1621 tcctgtacag tgatcgttcg ggcttgccca ttgagttcga aaaacacctt gcgatttccg
     1681 tctttatccg cctggctcac cgacaaaagc cgcacgatga tacttttgcc ctgtgcgatt
     1741 tcgatgatgg cttcttcgtt aggtttcata ccgaagaaga atatttcaga cggaataaca
     1801 gagacctgac cccatttttg ctgattttca aaataatcgg taaacacttt gggataaaat
     1861 ttgtaagaca aaaaatccgt aatcgaaaga ttggcatcga actgttgttt gaatgcttca
     1921 aactctttat caaaatccac gggcggca
//
LOCUS       DAOQNQ010000457         1921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_41149_length_1921_cov_5.419614, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000457 DAOQNQ010000000
VERSION     DAOQNQ010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41149_length_1921_cov_5.419614"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PK754_16290"
     CDS             <1..263
                     /locus_tag="PK754_16290"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNB11114.1"
                     /translation="CETSVNTKISQKNIPAIPEAFLNSGASLVLSTLWKINDRDAVNF
                     VDQLYQHWVIEKSIPAALQKTKRDFIVQNTLPFYWASWVIWK"
     regulatory      320..503
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            586..>1921
                     /locus_tag="PK754_16295"
     CDS             586..>1921
                     /locus_tag="PK754_16295"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNB11115.1"
                     /translation="MMKSTIYIGLLWFAIAEYYSQTGDSVKIKQYDLQGTVITSSRLI
                     NSRNASSALVNVITEENIQRRGATNLSDALSGASGIQIKDFGGTSGLKLPSFRGSTSE
                     QVIIMIDGIPLNNPATGLIDLGVVPLTMIDHIETYKGGDNNAVGGTINLITKKQPRDE
                     PMSIITKAHYSSFKRRWLSAGLTGFIKQTSYSVAYEFEYQPDSSYFVTVPSSGQRVPR
                     VNSESKRHALFLNVNNRLSLKTDFSLMSQWTFRDVEIPGTISNNTSVTSRGNQEDRVF
                     LIAPQIQHRFNEQWLTAIGSSWYKYQFDYTNSFPTVIHSRNISNSYHTRWTNLITLLK
                     QHTFNLGVTHTFVQGEGDNTDHVSNQSVSVFADDQWLIPLFNPMWSLRLSPAIRWDKF
                     SDFGQVWSPKAGMNIAYEKSGYYTSLRVSWSRNFRTPTLNERYWKDTDASGNP"
BASE COUNT          573 a          379 c          410 g          559 t
ORIGIN      
        1 cttgtgaaac atccgtaaac actaaaatat cacagaaaaa tatcccggct atacctgaag
       61 cgtttcttaa ttccggtgcc tcgttagttt taagtacctt atggaaaatt aacgatcgcg
      121 atgcagtaaa ttttgttgat cagttgtatc agcattgggt tatcgaaaaa tccattccgg
      181 cggcactaca aaaaaccaaa cgcgatttta tcgttcaaaa cacgcttcct ttttattggg
      241 catcgtgggt gatttggaag taattatttt aaaacttgac aaagaaaagt tttcggttat
      301 cttgagaact gtcaattgtt ctttgctatt tagtttaggt gtcacgcata cgacgtgatg
      361 aaaagggaat catgtgaaaa tcatgagctg ccccgcaact gtaagcgcat tctaataagt
      421 ccactgttat cgaatttatc ggtaatggga aggcaatctt taacgcgcaa gtcaggagac
      481 ctgcctaaac tgcattcgac caaccttcga ggggaaggcg tggggatcaa taaagtcaac
      541 ttttctatta tcctcattct ttccgaatga ggattttttt ttgatatgat gaaaagtacc
      601 atatacatcg gattattgtg gttcgccatt gccgaatatt attcgcaaac cggggatagt
      661 gttaaaatta agcaatatga cttgcagggt acggttatta cctcatcacg tttgataaat
      721 tccagaaatg cctcttctgc attggttaat gtcataacag aggaaaatat ccaaaggcga
      781 ggggctacaa acctgtccga cgctttgagt ggcgcttcag ggatacaaat taaagacttc
      841 ggcggaactt caggactcaa acttccatct ttccgtggct ctacttctga gcaggttatc
      901 atcatgattg acggaatacc gctgaataat ccggctacgg gcttaattga tttgggtgtt
      961 gttcctttga ccatgattga tcatatcgaa acatacaaag gtggcgacaa taacgccgta
     1021 ggtgggacaa taaaccttat aacaaaaaaa caaccacgtg atgaaccgat gtcaattata
     1081 acgaaagcgc actatagttc atttaaaaga cgatggttat cagcggggct aacaggtttc
     1141 ataaagcaaa cgtcttatag tgtcgcctat gagttcgaat accaacccga tagcagttat
     1201 tttgttaccg tgccgtcctc aggacaacgt gtaccgcggg tgaattcgga aagcaaacgt
     1261 catgctttat ttctcaatgt taataacaga ttaagcttaa aaacggattt ttctttgatg
     1321 agtcaatgga cgtttcgtga tgtggagata cccggtacga ttagtaataa tacatctgta
     1381 acatcgcgcg gaaatcagga agatcgtgta tttctcatcg cgccgcagat acaacaccgc
     1441 tttaacgagc aatggcttac ggcgataggc tcgagttggt ataaatatca gtttgattat
     1501 accaattcat ttccgacggt tatccatagt aggaatattt ctaatagtta ccatacacgt
     1561 tggactaatc tgataactct tctaaagcaa catactttca atttaggtgt tacccatacc
     1621 ttcgtgcaag gtgaaggtga taatacggat cacgtgagta atcagagtgt cagtgttttt
     1681 gccgacgatc aatggctaat tccgttgttc aatccgatgt ggtctttacg attatcaccg
     1741 gctatacgat gggataaatt ttcagatttt gggcaggtat ggagtcccaa ggccggaatg
     1801 aatattgcct acgaaaaatc aggctattac acgtccttac gcgtatcatg gtctcgcaat
     1861 tttagaacgc ctacgctcaa tgaacgatat tggaaagata cggacgcatc aggcaatccg
     1921 g
//
LOCUS       DAOQNQ010000458         1911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_41592_length_1911_cov_3.614224, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000458 DAOQNQ010000000
VERSION     DAOQNQ010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41592_length_1911_cov_3.614224"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..381
                     /locus_tag="PK754_16300"
     CDS             157..381
                     /locus_tag="PK754_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNB11116.1"
                     /translation="MQKTAVRKIGNSLGITLPKTVLENFHLSEGDELHLIETNEGIIL
                     SPFNPDFSEWATAYDQVNRKYRNTLHKFAK"
     gene            381..776
                     /locus_tag="PK754_16305"
     CDS             381..776
                     /locus_tag="PK754_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015127164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HNB11117.1"
                     /translation="MAVKYPPIELVKSIHANQIRQYGGIFGLRDPGLLESALAQPRRS
                     VGGKLIHKTIFDKAAAYGFHLCKNHPFVDGNKRVAFIIMYIFMANNNWIINASEESAY
                     SMMMELAEGKLSKRRLSAWLQKNSHKSLK"
     gene            1070..>1911
                     /locus_tag="PK754_16310"
     CDS             1070..>1911
                     /locus_tag="PK754_16310"
                     /inference="COORDINATES: protein motif:HMM:NF024886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNB11118.1"
                     /translation="MMHSHLKNKMIRLDAITRNRENHVLIRFGFDEELIALVKQLPDI
                     RWDSADKAWQTPNTKENVNRIFLLFKGKAWIDYSSLKSQKPEIKPARKNAHVKIILSK
                     QLNEEVLGKLEQFERWMQSKRYGENTIKTYTDALRTFLRFHHSVPLDKITNDHIIKFN
                     VEYITKGHYSAAFQNQVVNAVKLFFSKIENTVLDLKSVHRPRREKLLPNVLSKQEVKA
                     ILDAPKNIKHKAMLSLIYSCGLRRSELLNLKITDIDSKRGLVIIKQSKGKKDRVVPLG
                     LKIL"
BASE COUNT          612 a          358 c          392 g          549 t
ORIGIN      
        1 ttattatacc cgcggattat gggaaaaaat tcgtcggtcg cttcgtaaac tgccgtttcc
       61 tgttttttat gtaagatcat gccgccggta tggatagcca aaacttgaca ttacgtaata
      121 cacttgttat aatataacaa gtgaaaggat caagtcatgc aaaaaaccgc agtacgcaaa
      181 attggaaata gtcttggtat cacactgcct aaaactgttc tcgaaaattt tcatctctcc
      241 gaaggcgatg aacttcattt gatcgagaca aatgaaggca tcattttgtc gccatttaat
      301 ccggattttt cggaatgggc cacggcgtat gatcaagtta accgaaaata caggaatacg
      361 ctgcataagt ttgccaaata atggctgtaa aatatcctcc cattgaactt gtaaaatcaa
      421 ttcatgccaa tcagattcga caatatggcg gaatttttgg gcttcgtgat cccggacttt
      481 tagagtcggc tctggcacaa cctcgaagga gtgtgggtgg caaactaatc cataaaacga
      541 tttttgataa agccgccgcg tatggctttc atctttgcaa aaatcatcct ttcgtggatg
      601 gaaacaaacg cgtcgctttt atcattatgt atatattcat ggccaacaat aattggataa
      661 tcaacgcttc ggaagagtct gcttacagta tgatgatgga gcttgcggaa ggtaagctct
      721 caaaacgcag gctctcagcc tggctccaaa aaaactccca caaatcactc aaataacacg
      781 gcggaatacc ttatagtgtt atgcggcttg gcggattttt accgcaaaag tttaatagaa
      841 ttacggcctt tgaaccttgc acgaatattt tattgaagcg cgctttcatt tctcttttga
      901 aaatcggtac ggttcggctg tatcaaaatg atgtttgaaa aacttttttc tcgatcgcgt
      961 ttctataaat agtcattgac aaatgaaacg cgtacatgta catttaagca tgtagcctgt
     1021 ctggtatgcc acgtctcgaa gggttctttg gttaacttat atttttgaca tgatgcatag
     1081 ccatttgaaa aataaaatga ttcgtttaga tgcgattacc cgcaacaggg aaaatcatgt
     1141 tttgatcaga tttggttttg acgaggaact gatagctctt gtaaaacaac ttccggacat
     1201 ccgatgggac tctgccgata aggcttggca aacgccgaat accaaagaaa atgtcaatcg
     1261 cattttttta ctttttaagg gtaaagcgtg gattgattac agttcattga aatcccaaaa
     1321 accggaaata aaaccggcac gaaaaaatgc tcatgttaag attattttat cgaaacagtt
     1381 gaatgaggag gttctaggaa agcttgagca atttgagaga tggatgcagt ccaagcgata
     1441 tggtgaaaac acgatcaaaa cctacaccga tgcgttacga acatttttgc ggtttcatca
     1501 tagcgtacct cttgataaaa tcaccaatga tcacattatt aagtttaatg tcgaatatat
     1561 cacgaagggg cattattcgg cggcctttca aaatcaggtg gtcaacgccg taaaattatt
     1621 tttcagcaaa attgaaaaca ccgtattaga tttgaaaagc gtccataggc caaggcgtga
     1681 aaagttatta cccaatgtac tcagtaagca agaggttaaa gccattttag acgcgcctaa
     1741 aaatatcaag cataaagcga tgttgagttt gatatacagt tgcggtttac gaagaagcga
     1801 attactaaat ttaaaaatta cagatattga ttcgaagcga ggtttggtca tcataaaaca
     1861 gtctaaaggg aagaaagacc gggtcgtccc attaggtttg aaaatattac a
//
LOCUS       DAOQNQ010000459         1893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_42397_length_1893_cov_5.775843, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000459 DAOQNQ010000000
VERSION     DAOQNQ010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42397_length_1893_cov_5.775843"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PK754_16315"
     CDS             complement(<1..636)
                     /locus_tag="PK754_16315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNB11119.1"
                     /translation="MFKGSVLIVDDEDNLRQLMARMLELEDFGVIQSKDKASAKTVLE
                     HEPVDVMLLDVKLPDGFAIDWISELKSEFPCMEIVLLTAYGTIPDGVKAIKSGAYEYI
                     TKGTDNDQILPIVSRASEKARLNRRIQFLQSSAEEKHAFETVIGTSPAIRECINIARR
                     VAETDTTVLLSGETGTGKEIFAKAIHYGSPRKHKPFVAVNCSAFSRELLESE"
     gene            758..>1893
                     /locus_tag="PK754_16320"
     CDS             758..>1893
                     /locus_tag="PK754_16320"
                     /inference="COORDINATES: protein motif:HMM:NF023855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HNB11120.1"
                     /translation="MQKPAPPEPIRLDRETCGDYYYATQKEWLETNGLGGFASSTIIG
                     AHTRRYHGLLCASLRPPVQRFVFLSKYDEQVRIGDKLYDLATNQYPFIIHPDGHKRMA
                     SFEYDLYPRMIYDLDGVLLEKAILMPHGSNTVIVWYHLRESSQPVTLILRPLCAFRDY
                     HSLSRETSQIRKEAEFSEHRLTIRPYDHLPPLYITAPGAFFDPKFYWYNNTEYRKEQE
                     RGLEFREDLFTHGTLSVTLEPGSALDVAASLENTRFTSETIWEMWTNEVHRRIKILSD
                     FPLKDPLARRLALAADQFMVTGQSGKTIIAGYHWFTDWGRDTMISLPGLTLATGKTED
                     AKAIISTFLPFVSQGMIPNRFPDAGEAPEYNNVDGTLWFVMACY"
BASE COUNT          456 a          485 c          419 g          533 t
ORIGIN      
        1 ttctgattcc aaaagctctc ggctaaacgc gctgcaattc acggcgacaa aaggcttatg
       61 tttacgcggg ctaccgtagt gaatcgcttt tgcaaatatt tcttttcccg ttcccgtttc
      121 accggaaagc aagactgtag tatcggtttc cgccacgcgt cgggctatat ttatacattc
      181 ccggatggcc ggtgatgtac cgattacggt ttcaaatgcg tgtttttctt ctgccgatga
      241 ttgcaaaaac tgaattcgtc tatttaggcg tgctttttcg gacgcgcggc ttacgatagg
      301 cagtatctga tcgttgtccg tacctttggt gatatattcg taggcaccgg attttatcgc
      361 tttgacaccg tcaggtatag taccgtaggc cgtaagcaat acgatctcca tgcacggaaa
      421 ctccgacttg agttcggata tccaatcaat agcaaaaccg tccggtagct tgacatccag
      481 taacatcaca tccacaggct cgtgctcaag tacggtttta gctgaggctt tatctttgga
      541 ctgaatgacg ccaaaatctt caagttccag catacgcgcc ataagttgcc gcagattgtc
      601 ttcatcatct acgatgagca cgcttccttt gaacatacgg attaatttct gatgctttat
      661 ttcaaatttc ttttaactta tatagttctt accataataa caagttgccg catggcgcat
      721 atttccctgt ttaccgatta gttatcagga ttttaccatg caaaaaccgg cgcccccgga
      781 acccatacga ttggaccgcg aaacctgcgg tgattattat tacgccacgc aaaaggaatg
      841 gctggaaacc aacggtcttg gcggttttgc ttcgtccacg atcatcggtg cgcatacacg
      901 ccgatatcac ggtttattat gcgcctcgct tcgtccgcct gttcaacgtt ttgtcttttt
      961 atccaaatac gacgagcaag tacgtatcgg cgacaaactg tatgacctcg caaccaatca
     1021 atatccgttt attattcatc ctgatggcca taagcgcatg gcgtccttcg aatacgatct
     1081 ttatccgcgc atgatctacg acctggacgg tgtactgctc gaaaaagcca ttctcatgcc
     1141 gcatggaagc aataccgtga tcgtttggta tcaccttcgc gaatccagtc agcccgtcac
     1201 actgattctt cgccccttat gtgcattccg tgattaccac agcctttcac gcgaaacatc
     1261 gcaaattcgt aaagaagcag aattttccga acatcggctg accattcgtc cttacgacca
     1321 tctgccgcct ttgtacatca ccgcgcccgg cgcttttttt gatcctaaat tttattggta
     1381 caataacacc gagtaccgta aagagcagga acgcggtctc gagtttcgcg aagatctatt
     1441 tacgcacggc actctttcgg tcacattgga acccggatca gctttagatg tcgctgcctc
     1501 gctggaaaat acccgcttta cctctgaaac gatttgggag atgtggacta atgaagttca
     1561 tcgtcgcatc aaaatattgt cggattttcc gttgaaagat ccgcttgccc ggcgattggc
     1621 gttggcagcc gaccaattta tggtgaccgg acaaagcggt aagaccatta tcgcaggtta
     1681 tcactggttt actgattggg ggcgcgacac gatgatcagt ttgccgggcc tcacgctggc
     1741 cacaggaaaa accgaagacg caaaggctat catttcgaca tttcttcctt tcgtatcaca
     1801 gggtatgatc cccaatcgtt ttccggacgc cggcgaagcg ccggaataca ataacgtgga
     1861 cggtacgttg tggtttgtga tggcctgtta tca
//
LOCUS       DAOQNQ010000460         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_42619_length_1888_cov_9.758320, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000460 DAOQNQ010000000
VERSION     DAOQNQ010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42619_length_1888_cov_9.758320"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            357..581
                     /locus_tag="PK754_16325"
     CDS             357..581
                     /locus_tag="PK754_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11121.1"
                     /translation="MSENGNQFSLSHKEFCKATGLNRQQAYFARYGQHFKTNGSKYEY
                     PPKLVKEVDWTYNERGRPVFAQSAVEKIKK"
     gene            595..1254
                     /locus_tag="PK754_16330"
     CDS             595..1254
                     /locus_tag="PK754_16330"
                     /inference="COORDINATES: protein motif:HMM:NF016302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HNB11122.1"
                     /translation="MEHSTSIVKLAEALCKFQSQIKAVKKEAENPFYKSKYATLNDDF
                     EVARPYLSENKLSVLQFPSGGVDGIGLTTMILHESGEYISESFTIKPTKADPQSIGSL
                     IKYMRRYALEAALSLTTRDDVEDDDGNAATGKTGASGESAAKQATEAKQQQAASKQQT
                     ETPKTFAELSQELIDIDHIDSLVAWWTNNQSNIQALPEPQRSTIVRMKDEKKNMLTAQ
                     K"
     gene            1297..1620
                     /locus_tag="PK754_16335"
     CDS             1297..1620
                     /locus_tag="PK754_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11123.1"
                     /translation="MDLEKEIIHLQSELTALSDRVKELESRKREAKKKLVIPTPTEVD
                     AYFKEIGAYEHNIGQRFCDRYNAVDWYYKKDIRIVDWKAVARLWMQGRESRGGNSGKD
                     QYSHM"
BASE COUNT          693 a          352 c          431 g          412 t
ORIGIN      
        1 tattggaaat tgattgccat agcgatgaaa atatagaata caaccgtatg taccttatta
       61 acacgcaaag aggcgtcggt atgtttgtag ataggaggga agtaaaatga taaaacatga
      121 caaaacaaaa gtatcaaagt gctacacggc gttattggtt cgcggtgaaa ttgatacatg
      181 gttcgccata aaacacttta acgccacaag attagcggca tacatctatc ggtttcaaaa
      241 agaacttggg atgaaaatag aaaaaaaact aatgtatagc cgcgatgggt cgcattacac
      301 gaagtacata tacagaggat ttaagcgcgg tattaaaaat aacaaaggat aaaaaaatgt
      361 cagaaaatgg gaatcaattt agcttatcgc acaaagaatt ttgcaaagcg acggggctta
      421 ataggcaaca agcgtacttt gcgcgatacg gtcaacactt caaaactaat ggcagtaaat
      481 acgagtaccc gcctaagctt gtaaaggaag ttgattggac gtacaatgag cgcggtcgcc
      541 cggtattcgc acagtcggca gtagaaaaaa taaaaaaata aaaaggagat tagtatggag
      601 catagcacgt caatagtaaa gcttgcagaa gcattatgta aattccaatc gcaaatcaaa
      661 gccgtcaaaa aagaagcaga aaatccattt tataaaagca aatatgcgac gctaaatgac
      721 gacttcgagg tggcgcgtcc ttacctttct gaaaacaagt tatccgtttt acaattccca
      781 tccggcggag ttgatgggat aggattgact acaatgatat tgcacgaatc aggcgaatac
      841 atttccgaat catttactat taaaccaacc aaagcagacc cgcaaagcat aggctcgctt
      901 ataaaatata tgcgccggta cgcacttgag gcggcattat cattaacaac ccgcgatgac
      961 gttgaggatg acgacggcaa cgcggccaca ggcaaaactg gcgcatcggg ggaatcagcg
     1021 gctaaacaag caaccgaagc caaacaacaa caagccgcgt ctaaacaaca aacggaaacg
     1081 cccaaaacat tcgccgaact atcccaagag cttattgaca tagatcatat tgacagcctc
     1141 gttgcatggt ggacaaataa tcaaagcaac attcaagcgt tgcctgaacc acagcgcagt
     1201 acgattgtta ggatgaaaga tgagaagaaa aacatgctga cggcgcagaa gtaagataaa
     1261 taattcatat cggaatacga gacaaaggag tgatacatgg acttagaaaa agaaatcatt
     1321 cacctacaat cagagcttac cgcactttct gaccgcgtga aagaactgga aagcagaaag
     1381 cgtgaagcta agaaaaagct tgtcatacct acgccaacgg aagttgacgc ttactttaaa
     1441 gagatcggcg cgtatgagca taatataggt caacgatttt gcgacagata taatgctgta
     1501 gattggtatt acaaaaaaga tattcgtatc gttgactgga aggcggtggc aaggttatgg
     1561 atgcaaggaa gagaatcacg cggcggtaat agcggcaaag atcaatattc gcatatgtaa
     1621 catcactgcg gctacacgaa gccgccaaga gtaaacaaca aattaaccgc gagcggtttt
     1681 cgtgtgagcc gcttgttatg cgaggtcgag agatgataac tgttgaatat aataaaagcg
     1741 aacacacatt tgttgtcagc ggagaaagga tagaaaacgg ttatttttta tttgtaattg
     1801 aacacggatc aatagagacg ataaaaaggg ctattagaga gcgacttgaa aagcgtagga
     1861 atataaatgg caatctcgaa taacggtt
//
LOCUS       DAOQNQ010000461         1872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_43362_length_1872_cov_5.907540, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000461 DAOQNQ010000000
VERSION     DAOQNQ010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43362_length_1872_cov_5.907540"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PK754_16340"
     CDS             <1..383
                     /locus_tag="PK754_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11124.1"
                     /translation="FVYEALRDSSRLKSWWTDWAGSEPINDSAHVIHEIIDSEKSEFV
                     LTDRIAWPGILLTYGYEDAEKKGFLEYRLEYMGDSTRVTLRHRTRGYSWIQRSLLALR
                     YTDDKLRWRRYLYNFKRMMENDSI"
     gene            complement(389..1405)
                     /locus_tag="PK754_16345"
     CDS             complement(389..1405)
                     /locus_tag="PK754_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015079866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNB11125.1"
                     /translation="MDLTTSYLGMTLKNPIVPSASPFSKDIVQIKRMEEAGAAAVVLH
                     SLFEEQITHDEREMFYHLHHGSESSPEAQSYFVDMDRYNRGPEWYLEHIHRTKAAVGI
                     PIIASLNGSSHSGWLDYAKKIEQAGADALELNMYYIAADIAESATSIEHRYLEIARTL
                     RQSLRIPIAIKLSPFFTSFAHFAKHMDDCGINGLVLFNRFYQPDINLETLDVMPNVIL
                     SQSTELRLALRWIAILHGRIRADLAATGGIHTHEDVIKALLAGASVTMVCSTLLKNGI
                     ARITDIVKGMETWLVEREYESVKQLQGSMSQKSVSDPTAFERANYMRALSGYNLPNDL
                     LYHN"
     gene            complement(1455..>1872)
                     /locus_tag="PK754_16350"
     CDS             complement(1455..>1872)
                     /locus_tag="PK754_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNB11126.1"
                     /translation="IAMGANDAQTVKALLEAEAYDGPSLIIAYSHCIAHGINMGQGMR
                     QQKSAVDCGHWLLYRFNPDHVSLGQNPLTLDSKAPTLAFKDYALSETRYKMLAMTDPS
                     ASESLVKQAQQDILQKWHFYEQMAKLHYNGQNEEKH"
BASE COUNT          459 a          404 c          492 g          517 t
ORIGIN      
        1 tatttgtata tgaagcgctg cgagattcgt ctcgtttgaa aagctggtgg acagattggg
       61 cgggaagcga gccgatcaat gattctgcgc atgtgataca tgaaatcatt gacagcgaaa
      121 aaagcgaatt tgttcttacg gacaggatag cgtggcccgg cattttgctt acgtatggtt
      181 acgaagatgc cgagaaaaag gggtttttag aatacaggct ggaatatatg ggtgattcga
      241 cgcgtgttac gttgcgtcat cgcacgcgcg gctactcatg gatacagcgc tcactattgg
      301 ccttgcgtta taccgatgat aaactgcgat ggcgacgata cctttataat ttcaaacgca
      361 tgatggaaaa cgattcgatc tgatgcggtc aattgtgata caaaagatcg ttgggcagat
      421 tatagccgct taatgcccgc atgtagttag cccgttcaaa ggctgtggga tcggaaacgg
      481 atttttgact catgctgcct tgcagttgtt ttaccgattc gtattctctt tcgacgagcc
      541 atgtttccat gccttttaca atatccgtga tacgtgcgat tccgtttttg agcaacgtgg
      601 aacaaaccat ggtgaccgat gcaccggcga gtaatgcttt gatgacgtct tcatgcgtat
      661 gaatgccgcc tgtcgccgcc aggtcggcgc gaatgcgccc atggagaatg gcaatccatc
      721 gcagagctaa gcgtaattcc gttgattgac tcagaataac attcggcatt acatcaagcg
      781 tttccagatt gatatccggt tgataaaatc gattaaaaag tacaaggccg ttgatgccgc
      841 agtcatccat gtgtttggca aaatgtgcaa aagaagtgaa gaacggactg agtttgatcg
      901 cgatgggtat gcgaagactt tggcgtaaag tgcgtgcgat ttccagataa cgatgttcga
      961 tagaggtcgc ggattcagcg atatcggccg caatgtagta catattgagc tccagcgcat
     1021 cggctccggc ttgttcgatt tttttggcat aatccagcca cccggaatgc gatgatccgt
     1081 tgaggctggc gatgatggga atgcccacgg cggctttggt gcgatgaata tgctccagat
     1141 accattcggg tccgcggttg tagcgatcca tatccacaaa ataggattgc gcttcgggcg
     1201 aactttccga tccgtggtgc aaatgataaa acatctctct ttcatcatga gtgatttgct
     1261 cttcgaataa cgaatgtaaa accaccgcgg cggcgccggc ctcttccatg cgtttgattt
     1321 gcacgatgtc tttggagaaa ggcgaagcgg aaggaacgat cgggtttttg agcgtcattc
     1381 cgagatagct ggtggttaag tccatagtgc gtcctcaaga aaattttgtg gaatgcgaaa
     1441 ttctgaattc acgtctaatg tttctcttcg ttttgtccgt tgtaatgcaa tttcgccatt
     1501 tgttcgtaga agtgccattt ttgaagtatg tcctgttgcg cttgtttgac caaactctcc
     1561 gatgccgatg gatccgtcat ggccagcatt ttataacgcg tttcgctcag agcataatct
     1621 ttgaatgcaa gcgtaggcgc ttttgaatcc aatgtgagag ggttttgacc cagagacaca
     1681 tgatccggat tgaaacggta caataaccaa tgaccgcaat cgacggcgga tttttgctgg
     1741 cgcataccct ggcccatatt aataccgtgt gcgatacagt ggctataggc gatgatgagt
     1801 gaaggtccgt cgtaagcttc ggcttccaat agcgctttga cagtctgtgc atcgttggcg
     1861 cccatcgcga tc
//
LOCUS       DAOQNQ010000462         1871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_43417_length_1871_cov_4.613987, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000462 DAOQNQ010000000
VERSION     DAOQNQ010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43417_length_1871_cov_4.613987"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..796)
                     /locus_tag="PK754_16355"
     CDS             complement(26..796)
                     /locus_tag="PK754_16355"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNB11127.1"
                     /translation="MSALVIGKYSFNSRLIVGTGKYKDYDETKKALEESGAEMITVAL
                     RRVDLTKPDDKNLLDYIGSKYTLLPNTAGCYTAKDAVLTCKLAREALGATLVKLEVLG
                     DPRTLFPDNEQLLEAAQILVKDGFTVLPYCIDDPIVCKKLEDIGCAAVMPLAAPIGSG
                     LGIRNPYNLAIIIEHSKVPVIVDAGVGTASDAAIAMELGCDGILMNTAIAGAKDPIKM
                     ARAMRLAVEGGRLAYEAGRIPKKLYATASSPIEGVIKT"
     gene            complement(889..1092)
                     /gene="thiS"
                     /locus_tag="PK754_16360"
     CDS             complement(889..1092)
                     /gene="thiS"
                     /locus_tag="PK754_16360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNB11128.1"
                     /translation="MKIKINGTVYELDSVKTIDDVLQHFGVSYAHGVAVAVNYDVISK
                     DRIQTQTVCEGDEIEIIRATQGG"
     gene            complement(1095..1595)
                     /gene="def"
                     /locus_tag="PK754_16365"
     CDS             complement(1095..1595)
                     /gene="def"
                     /locus_tag="PK754_16365"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNB11129.1"
                     /translation="MKVVLYGDPILRSVCKPVVAITDTLRMTVANMIETIGEHQGVGL
                     AAPQVGMSERLVVIDPRLMDRTEEVIVMINPEIRESEGSNVFREGCLSLPGVEAEIRR
                     PAAVTVSYTDMDNVSQTKRFIGVKARIVQHEIDHLNGVLFTDYLNPVWRWHLLRKVKR
                     RINGKA"
     gene            complement(1607..>1871)
                     /gene="yajC"
                     /locus_tag="PK754_16370"
     CDS             complement(1607..>1871)
                     /gene="yajC"
                     /locus_tag="PK754_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNB11130.1"
                     /translation="FIIAIMYFMMIRPQQKRQKEHQAMVSALAKGDKVITVGGIHGVV
                     QGTKETTVLVKIADNVKVEIERSAVTTVVKGSGEASAEAKSAE"
BASE COUNT          494 a          470 c          375 g          532 t
ORIGIN      
        1 tgttattgag ggcatttgca agcatttagg tcttaataac gccctctata ggactggaag
       61 ccgtagcata aagttttttc gggattcgcc ccgcttcata agccagacgt cccccttcga
      121 cggccaaacg cattgcgcgt gccattttga tgggatcttt tgctccggcg atagcggtat
      181 tcatgagaat accatcgcaa ccaagctcca ttgcaatcgc tgcatcggaa gctgtaccga
      241 cacccgcatc aacgatgaca ggcactttgg aatgttcaat gataatagca agattgtacg
      301 gattacgaat tcctaaaccg gaaccaatcg gcgcagctaa cggcatgacg gcggcacaac
      361 cgatgtcttc cagtttttta caaacgatcg ggtcatcaat acaatatggc aaaacggtaa
      421 aaccatcttt gactaaaatt tgtgcggctt cgagaagctg ttcattatcc ggaaataatg
      481 tacgggggtc acccaaaacc tccagcttga ccaatgtcgc acctaaggct tcccgtgcca
      541 gtttacatgt cagaaccgca tctttagccg tgtaacaacc ggccgtatta ggaagtaacg
      601 tgtattttga accgatataa tcgagcagat tcttgtcgtc gggtttagtg agatcaacac
      661 gtcgaagcgc cacggtgatc atttctgcgc cactctcttc aagcgctttt ttagtttcat
      721 cgtaatcttt atattttccc gtaccgacaa tcaaccgcga attgaaggaa tatttgccaa
      781 ttaccaacgc actcatattt tattttccta aatattcttt tttgaatata cgcgttttaa
      841 taaaaatagc aaaccccgcc gtaaagcggg gtttatataa actaagcttc agccgccctg
      901 tgttgctcgt atgatttcga tttcatcgcc ctcgcatacc gtttgtgttt gaatgcgatc
      961 tttggaaatt acgtcgtaat taacagctac ggcgacacca tgtgcatatg aaacaccaaa
     1021 atgttggagt acatcatcaa ttgtttttac agaatccaat tcgtaaaccg taccattgat
     1081 tttaatcttc atagctaagc cttaccgtta atccgacgtt ttacttttcg caataaatgc
     1141 caccgccaca cagggttcaa ataatcggta aataagacac cgttgagatg atctatttcg
     1201 tgctgtacga tacgggcctt tactccgata aaacgcttcg tctgtgatac gttatccata
     1261 tcggtataac taactgtaac tgcagcaggg cgccttattt cagcctcaac ccctggaagg
     1321 ctcagacagc cttcgcgaaa tacattggaa ccttcgcttt cgcgtatttc cggattgatc
     1381 atcacgatca cttcttcggt gcgatccatt aggcgcggat caatcacgac aagacgttca
     1441 ctcatgccca cttgcggtgc cgccaaaccg acgccttggt gctcaccaat cgtttcgatc
     1501 atattcgcga ccgtcatgcg taacgtgtcc gtgatagcaa cgaccggttt acaaactgaa
     1561 cgcaaaatgg gatcgccgta taaaaccact ttcacaatga aatcggttat tcggccgatt
     1621 ttgcttcagc ggatgcttcg ccggaacctt tgacaacggt ggttaccgcc gaacgttcga
     1681 tttcgacttt tacattatca gctattttta ccaataccgt cgtctctttg gtaccttgga
     1741 ctacaccatg gataccgccg accgtaatta ctttatcccc tttcgccaaa gcgctcacca
     1801 tcgcttgatg ttctttttga cgtttttgct gaggacgaat catcatgaaa tacatgatcg
     1861 caatgataaa t
//
LOCUS       DAOQNQ010000463         1871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_43419_length_1871_cov_4.252753, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000463 DAOQNQ010000000
VERSION     DAOQNQ010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43419_length_1871_cov_4.252753"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="PK754_16375"
     CDS             complement(<1..1016)
                     /locus_tag="PK754_16375"
                     /inference="COORDINATES: protein motif:HMM:NF021427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HNB11131.1"
                     /translation="MSEQIICPKCKHQFELSDAFQSRFEAQAEQKIRDEFNQKFKSEI
                     AKREEKIRRDTMEQWTLQLADLQQQLAIKDKNMQDARTRELQLMKQQREIEEREQNLK
                     LETERLIMEERNKIRLEVEAKAIEAFRLKDAEKDKRIADMMRQVDELKRKAEQGSQQS
                     QGEVMELALENLLREKFPLDVISEVPKGIRGADVLQRVYNRSGLESGTIIWESKRTKE
                     WSPSWIQKLKDDMRSAGANVAVLVTQALPKEIKTMGNLDGVWITGFGTAVELAEVLRV
                     GMTELAQTRIAAVGKNEKMEMLYNYLTGNEFRQKMEAVLEAFVQMRGDLDQERRSMEK
                     NWQRR"
     gene            1181..>1871
                     /locus_tag="PK754_16380"
     CDS             1181..>1871
                     /locus_tag="PK754_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11132.1"
                     /translation="MSDYFRRIVLCLGVSLIGVLPLRAQGTLYRGTQSLFMETGVGAR
                     AQALGRAFTGLANDGSAVFWNPAGLDFLQQPNFVAYHSTLVEGTFFNFAAVTYPFLEF
                     GSVGVGMARIGVNGIPLNNEWTNLAQGEYSEQEYYIAYGKRLPFWNLSLGTTFKMVNV
                     NASELNANDAPVSKSGFSSGLDIALMWKPDFENAIVRNMSFGFNYQNVVAPSFKLSTF
                     SYNDPRNLRFGI"
BASE COUNT          418 a          461 c          384 g          608 t
ORIGIN      
        1 cggcgttgcc agttcttttc catgctgcgg cgctcctgat ctaaatcgcc gcgcatttgc
       61 acaaaagctt caagtacagc ctccatcttt tggcggaact cattgcccgt aagatagttg
      121 tataacattt ccattttctc atttttacca accgccgcaa tacgtgtttg cgcaagctcc
      181 gtcattccca cacgcaaaac ttcagccagt tcgaccgctg ttccaaatcc tgtaatccag
      241 acgccgtcca gattgcccat cgttttaatt tctttcggta aggcttgggt aaccaaaacg
      301 gctacattgg ctcctgccga acgcatatca tctttgagct tttgaatcca cgatgggctc
      361 cattctttgg tacgtttcga ttcccatatt atggttccgg attccaagcc cgatctgttg
      421 taaacccgtt gcaaaacatc ggcgccccga attcctttgg gaacttcgct gataacatca
      481 agcggaaatt tttcacgtag taaattctcc aaagccagtt ccatgacctc cccttgggat
      541 tgctgtgaac cttgttcggc tttgcgtttg agttcgtcca cttgacgcat catatcggca
      601 atgcgtttat ctttctcagc gtctttgagc cgaaatgctt cgatcgcctt cgcttcgact
      661 tcaagtcgga ttttattacg ctcttccata atcagacgtt cggtttccaa cttcaaattt
      721 tgttcgcgtt cttcgatctc gcgttgctgt ttcatcaatt gcaactcgcg ggtacgggca
      781 tcctgcatgt tcttatcttt gatagccagt tgttgctgta gatcggccaa ctgcaatgtc
      841 cattgctcca tcgtatcgcg acgaatcttc tcttcgcgtt tggctatttc ggacttaaat
      901 ttctgattaa attcgtcccg gattttttgc tcggcctgcg cttcaaagcg actttgaaag
      961 gcatctgaaa gttcaaactg atgcttgcat ttgggacata taatctgctc actcatgata
     1021 actccttaca taaacaacgc cgccatgata cgatgatttg caatgatttt ctatcatatt
     1081 ttttcacaat tttttctatt gcgtattcga gcgtataact gtatttttat tgaaaatagc
     1141 acacttcggt ttttttacgt ttcaagtaaa gcgcttgccc atgtcggatt atttccgccg
     1201 tatagttttg tgtctgggtg tatctttgat aggtgtattg ccgcttcgcg cacaaggcac
     1261 gctctatcgt ggcacgcaga gtctttttat ggaaaccggt gtcggcgcac gcgcacaggc
     1321 tttgggtcgt gcatttacgg ggctcgccaa tgacggttca gccgtttttt ggaatccagc
     1381 cgggttagat tttttgcaac agcctaattt tgtagcctat cattccaccc ttgtcgaggg
     1441 tacttttttc aattttgccg ccgtaacata tcctttttta gaatttggct ccgtcggagt
     1501 cggtatggcg cgtatcgggg tcaatggcat ccctctcaat aacgagtgga ctaatctggc
     1561 tcaaggcgaa tactcagaac aagaatacta tatcgcgtat ggcaaacgcc taccattttg
     1621 gaatttatca ttaggtacga cttttaaaat ggtcaatgtc aatgccagcg aactgaatgc
     1681 caatgatgcg ccggtttcga aatccggatt ttcaagcggt ctggatatcg cactgatgtg
     1741 gaaacctgat tttgaaaatg caatagtacg caatatgtct ttcggtttta attatcaaaa
     1801 tgtagtcgct ccctccttca aactttccac attttcgtac aacgatcccc gcaatctgcg
     1861 ttttggtata g
//
LOCUS       DAOQNQ010000464         1870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_43460_length_1870_cov_11.276033, whole genome shotgun
            sequence.
ACCESSION   DAOQNQ010000464 DAOQNQ010000000
VERSION     DAOQNQ010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43460_length_1870_cov_11.276033"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1870
                     /locus_tag="PK754_16385"
     CDS             <1..>1870
                     /locus_tag="PK754_16385"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNB11133.1"
                     /translation="DPLLVINTNDAGAGSLRDAVTYAESHSGADTIRFAVALDGQSIT
                     LLNPIYFNGDSTTVTGDIDSDFIPDITLVRGASTNWGFAVNGNHNVISHLNFQQFNTA
                     GILINGHHNHIRGNTIGTNLDGSDNTGTGNTWGIQLVNSAKYNTIGDTVNAGRNIIAG
                     NTGYGIVFQNNSDSNAVIGNYIGLNAAGDNAFGNGTAGIVIDAGPKNNTIGNGLANGR
                     NIISGNNYAVFITGAGANYNRIIGNFIGTDPLGTTAIPNPSGGINIASGAKYNEVGNG
                     TVGGRNVISGNGSYEIVLSNSDSNKISGNYIGLDVTGANGISTAISGISASNVHGTVI
                     GGVQANDRNYFGDAGYGVSFSGGSDNVIRNNSFGSDITGANAIGSATALYFNSSAHRD
                     SVIDNRITRHLSSAVYIQSGDSIVLLGNQIHHNAGPGISMDPGAQSDIASPRIVSIAG
                     DSTLIGKAAPNAFVQVFADSLDEGEFSMGSTLANASGDWSLKVPVATGRQFTALQDSM
                     GRTSAFSAPYTPNFVPTAPTGLFALAFKDSVRLHWNPAPEAFTSKYYIYSDVTSNPTT
                     LVDSTTGGQMDTSKVITALSNGTQYYFRVSAVGPGNSVSAFSNEDAVVPVIEAGNAL"
BASE COUNT          479 a          390 c          486 g          515 t
ORIGIN      
        1 gatccgctat tagtcatcaa taccaacgat gccggtgccg gttcactgcg tgatgcggtg
       61 acgtatgcgg agtcccatag cggagcggat acgatacgat ttgcagttgc gcttgatggt
      121 cagtctatca cgttgttaaa tccgatatat tttaacggtg attcaaccac ggtcaccggt
      181 gatatagatt ctgattttat accggatatt accttggtac gcggcgcttc aacgaactgg
      241 ggctttgccg tcaatggtaa tcataatgtg atcagccatc ttaatttcca gcagtttaac
      301 acagccggta tccttattaa cggccaccat aatcatattc gcggtaatac gatcggtacg
      361 aatctggatg gctcggataa taccggtacg ggaaacacct ggggtatcca gcttgtaaat
      421 tcagcgaagt ataataccat cggtgatacg gttaatgcag gccgtaatat tattgccggt
      481 aataccggat atggtatcgt attccagaat aattcggaca gtaacgcggt tatcggaaac
      541 tatatcggtc tcaatgcggc aggcgataat gcctttggta atggaacagc gggtatcgtg
      601 atagatgcag gaccgaagaa taacacgatc ggtaatggtt tagctaatgg ccgtaacatt
      661 atttcaggta ataattacgc ggtattcatc acaggtgccg gcgctaacta caatcgcatt
      721 atcggtaatt ttatcggaac cgatccgctt ggaactacgg ctattcccaa tccttccggt
      781 ggtatcaata tcgcatccgg agcgaaatac aatgaagtag gtaatggcac ggtgggcgga
      841 agaaatgtta tatcgggcaa tggtagttac gagatcgtac ttagtaactc cgattcgaat
      901 aagatttccg gtaattatat aggccttgat gtcacaggag cgaatgggat ttcgacggcc
      961 atttcgggaa tatcggcttc taatgtgcac ggaaccgtta ttggtggtgt acaagccaat
     1021 gatcgtaatt actttggtga tgccggttat ggtgtatcct tctccggagg atcagacaat
     1081 gtaattcgca ataactcatt tggttcggat atcacgggag ctaatgcgat cggtagcgct
     1141 acggccttgt attttaacag tagtgctcat cgcgatagcg tcattgataa tagaataacg
     1201 cgtcatctca gttctgcagt ttacatccaa tcgggtgata gtatcgtact gttggggaat
     1261 caaatccatc acaatgccgg tcctggcata agtatggatc cgggagcgca gagtgatata
     1321 gcctcaccgc gcatagtatc catagccgga gattcgacct taattggtaa agcggctccg
     1381 aatgcttttg ttcaggtgtt tgcggacagt ttggatgaag gcgaatttag tatgggttcg
     1441 acgttggcca atgcgtcggg tgattggtca ttgaaagttc cggttgctac aggtcgtcag
     1501 tttacggcgt tgcaggatag tatgggtcgt acgtcggcat tttcggcgcc ttatacaccg
     1561 aactttgttc cgacagcacc gacgggtttg tttgcattag cattcaaaga cagtgtacgt
     1621 ttgcactgga accctgcgcc ggaggcattt acatcgaagt attacattta cagtgatgtg
     1681 acatccaatc cgacaacatt ggtggatagt acaaccggtg gtcagatgga tacgtccaaa
     1741 gtgataacag ctctttccaa tggcacgcag tattacttcc gagtctcagc tgttgggccg
     1801 ggcaattcgg ttagcgcatt ctccaatgaa gatgcggtcg tgccggttat agaagccgga
     1861 aatgcgttgg
//
LOCUS       DAOQNQ010000465         1835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_45191_length_1835_cov_4.944944, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000465 DAOQNQ010000000
VERSION     DAOQNQ010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45191_length_1835_cov_4.944944"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..>1835
                     /locus_tag="PK754_16390"
     CDS             64..>1835
                     /locus_tag="PK754_16390"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB11134.1"
                     /translation="MRNIVIRENDVFFIDRSAIFRYRDGIITSQKPKFVFGTGDKFGD
                     RIFINDFGPTEQRMLELVGDSLVVLPYFKNEARIHCLIPAGEGKFIASTGSGIYAWDH
                     GREYPIYQSKDVRRGIRLSDGGFAFRTRSEGVIILDSALRRVEIINEDVGLSANSTRD
                     VMEDRFGNLWVATEDGINKIDRSSLKILQKAQGLKGCAYKIAPMGDLNYIGTLNDLYL
                     IQNGKVVPQNIGRINGGVALAKGYNFASGRGIIHLKKNNNLQLIPNSPDAISIVALDE
                     RYLVLGKRLGITFYDVISNRETPTTAASKISGIGQLWYDRRGSVWAESEYGNTYRIDM
                     KDTSLHVYEGDWKMLLCADSLVFMQHKDTIFRYDFIRKSFIPNPSFFPESSNAVKAIA
                     DNAGDIWFFTNTAVWNGHYKETQWHWEKDPYYRIHNHTKQFPPNEVYRDARGMIWFLT
                     DDGVLRIGPEMVSQRQSFDVVISRFSVSNDSALIINDRRTVPITLPYALNSVRIEYGA
                     TSFIDENNTIFQTWLEGFESVPSGWTSDPVSVYSNLSEGEYVFHVSAMNAVRRIQTTE
                     LRFRVLPPWYRTWWAWLAYMMLFVS"
BASE COUNT          479 a          355 c          472 g          529 t
ORIGIN      
        1 agcggccgat cccgtacggt ggttttaaat tcgatagttc cgggcgatac gccggttgat
       61 ttagtgcgaa atatagttat tcgtgaaaac gacgtttttt ttattgatcg ctctgctata
      121 ttccgttatc gggacggtat cataacatca cagaagccta aatttgtttt tgggacgggc
      181 gataagtttg gtgatcggat ttttataaat gattttggtc cgacggaaca acgtatgctg
      241 gagcttgtgg gggactcgct tgtcgtcctg ccgtatttta aaaatgaagc ccgcattcac
      301 tgtctgatac cggcgggtga gggaaaattt attgcaagta cgggatccgg aatttatgca
      361 tgggatcatg ggcgtgaata tcctatttat caatccaaag atgtgcgccg gggaatccga
      421 ttgtccgacg gaggatttgc attcagaaca cgatccgaag gtgtgatcat actggattcg
      481 gcactgcgtc gcgtagagat cattaatgaa gatgtcggcc tttccgctaa ctccacccgc
      541 gatgttatgg aagatcgttt tggaaatttg tgggttgcta cggaggacgg aataaataaa
      601 atagatcgtt catcgctcaa gattttgcaa aaagcgcaag gattgaaagg gtgtgcttat
      661 aaaatcgcgc ccatgggtga tctcaactat atcggcacgc tcaacgatct gtacctgatc
      721 caaaacggaa aggttgttcc acaaaatatt ggacgaatca atggcggggt tgctcttgcg
      781 aaggggtata attttgcttc aggtcgcggg atcattcatc tgaaaaaaaa taacaacctt
      841 cagctgattc ccaatagccc ggatgccatc agtattgtag cactagatga gcgttatctc
      901 gtgcttggca aaagattggg aattacgttt tatgatgtta tttccaatcg cgagacgccg
      961 acgactgcgg cttccaaaat atccgggata ggacagctct ggtatgatcg gcgaggatcg
     1021 gtgtgggcgg aatcggaata tggcaatacc tatcggattg atatgaaaga tacgagcctt
     1081 catgtgtatg agggcgattg gaagatgttg ttgtgcgcgg actcattggt atttatgcaa
     1141 cataaggata ccattttcag atacgatttt atccgaaaaa gttttattcc taatccgtcg
     1201 ttttttccgg aatcatcgaa tgctgtcaaa gccatagctg ataatgccgg tgatatttgg
     1261 ttttttacga acaccgcggt atggaatggt cattataaag aaacacaatg gcattgggaa
     1321 aaagatccct attaccgtat tcataatcac accaaacaat ttccgcctaa cgaagtatat
     1381 cgtgatgcgc gcgggatgat ttggtttctt acggacgacg gtgtgctacg tatcggaccg
     1441 gagatggttt cacaacggca atcgttcgat gtggttattt cccgattcag tgtaagcaac
     1501 gattcagctt taattatcaa tgatcggcga accgttccta ttactttgcc atatgcattg
     1561 aattcggtac gcatcgaata cggggcgact tcatttattg atgaaaacaa tacgatattc
     1621 caaacatggt tagagggttt tgaatcggta cctagcgggt ggacttccga tccggtaagc
     1681 gtgtattcta atcttagcga aggggaatat gtttttcacg tatcggccat gaatgcggtg
     1741 cggcgcatac agacaaccga actgcggttt cgtgttctgc cgccgtggta tcggacgtgg
     1801 tgggcatggt tagcctatat gatgttgttt gtgtc
//
LOCUS       DAOQNQ010000466         1831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_45401_length_1831_cov_4.862050, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000466 DAOQNQ010000000
VERSION     DAOQNQ010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45401_length_1831_cov_4.862050"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PK754_16395"
     CDS             <1..354
                     /locus_tag="PK754_16395"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNB11135.1"
                     /translation="CYRYAAIYEIFKKNQMPVKQILASGGGFVDSGALTQLAADVIGF
                     PITQSGEAEGSARGAAILALESIGTIKNIDMAPVPLGKTYKPHPKDNAIYKNARQRQQ
                     RLYDMVAKGIWEENL"
     gene            459..887
                     /locus_tag="PK754_16400"
     CDS             459..887
                     /locus_tag="PK754_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11136.1"
                     /translation="MRQKWSLIVIVIVLLQCFLLKSCISNDQQDDLRVGLSVLTDKSL
                     YHNERIYIHISNKTESIFYLLLYNSAFREKIVKDQWSLVTSSLIDDPQSDTVIINMNE
                     ATVMDIFVVKPGIYRVGIPFLWNGDPGRKDTVYSNEFEVY"
     gene            1047..>1831
                     /locus_tag="PK754_16405"
     CDS             1047..>1831
                     /locus_tag="PK754_16405"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HNB11137.1"
                     /translation="MINQTDVIIVGAGAAGLMCAMTAARRGRRVVVLEQNEQVGKKIL
                     ISGGGRCNFTNLLSGPDNFISENPHFFKSALSRYSPTDFVELVKKHNIAFHEKKNGQL
                     FCDHSAKDIVSMLLSECADGGVRMVMKNRVEEVRYDECYTLQTNTGIYKAPHVVIATG
                     GLSIPKIGATDFGLRLAEKFGHRIVPPEPALVPLQMDAGFIQRYGDLTGISIEVIISC
                     EKKYFHENMLFTHQGISGPAVLQISSYWKKGDILTIDLLPNKT"
BASE COUNT          557 a          302 c          387 g          585 t
ORIGIN      
        1 tgttaccgtt atgcagcgat ttatgaaatc tttaaaaaga atcaaatgcc cgtgaaacaa
       61 atattggctt cgggtggagg ttttgttgat tcaggagcac tgacacaact tgcggcagat
      121 gtaataggat ttccgatcac ccaatccggc gaggccgaag gctctgcgcg tggagcggca
      181 attttggcat tagaatcaat aggaactatc aaaaatattg atatggctcc cgtacctctt
      241 ggaaaaacat ataagccgca tccaaaggat aatgcgatct ataaaaatgc cagacagcgg
      301 caacaacgat tgtacgacat ggttgctaaa ggaatttggg aagaaaattt gtaagtatta
      361 gaaccctcgg taacgagggt tttatttttt aaataatctg taagtttttg tgtctcacca
      421 gtactaagat aatagaaatt atcgagggag gatggattat gaggcaaaaa tggtctttaa
      481 ttgtgatagt gattgttcta ttacagtgtt ttcttctaaa gtcatgtatt tcaaatgatc
      541 aacaagatga tcttagagtt ggcttatcag ttttaactga taaatcacta tatcataatg
      601 agcgtattta tatacatatt tccaataaaa cggaatcaat tttttatttg ttactgtata
      661 attccgcctt tcgtgaaaaa attgttaaag atcaatggag tttggttact tcatcgctaa
      721 tagatgatcc tcaaagcgat acagttatta ttaatatgaa tgaggcaacg gtaatggata
      781 ttttcgtcgt aaaaccaggt atttatcgtg tcgggattcc ttttttatgg aatggtgatc
      841 ctggacgtaa ggataccgtg tattctaatg agtttgaagt atattgaagt ccagttattg
      901 aattttgaat tcttttttta agaaaattgt tataaacgca tttaattttt ttctttcatc
      961 attatccgtt tatttcagta ttttactcat cagtcataat accaattcga tctcaaaaga
     1021 gtaaaataat tgcacgttaa ttcactatga taaatcaaac ggatgtcatc atcgtcggcg
     1081 ctggcgcggc cggtctgatg tgcgctatga cggcggctcg aagaggacgc cgcgtcgtag
     1141 ttttagaaca aaatgaacag gtaggaaaaa aaattctgat ttcaggcggt gggcgttgca
     1201 atttcaccaa tcttttatcg ggacctgata attttatttc agaaaaccct cattttttta
     1261 aatcagcttt atcgcggtat agtcccacag attttgttga attggtaaaa aaacacaata
     1321 ttgcttttca tgaaaaaaag aacggtcagt tattctgtga tcattccgcg aaggacattg
     1381 tatccatgct gttgagcgaa tgtgccgatg gcggagtcag gatggttatg aaaaatcgtg
     1441 ttgaagaagt tcgttatgat gaatgctata cgttacaaac taacaccggt atttacaaag
     1501 caccccatgt cgtcatagcg actggagggc tttcaattcc aaaaataggc gctacggatt
     1561 ttggattgcg actggctgaa aagtttggtc atcggatagt cccgcctgaa ccggcgttag
     1621 ttcctcttca gatggatgcc ggttttattc agcggtacgg tgatttgacg ggtatttcaa
     1681 tcgaagttat tatttcatgc gaaaaaaaat attttcatga aaatatgctt ttcacacacc
     1741 agggtattag tgggccggcg gttttgcaga tttcttctta ctggaaaaaa ggagatattc
     1801 taaccataga tcttttgccg aataaaacga t
//
LOCUS       DAOQNQ010000467         1822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_45880_length_1822_cov_3.644029, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000467 DAOQNQ010000000
VERSION     DAOQNQ010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45880_length_1822_cov_3.644029"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="PK754_16410"
     CDS             <1..922
                     /locus_tag="PK754_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11138.1"
                     /translation="VNHLNSYNSNGDLKKRFTSDFELLIKNGMSNIISWFDKNIMGIT
                     SAYDSWINGFLKDAYAVLKKSELEKWYKSVFFRKYVVFCFLFSLISIPFGIYLDFNLL
                     LLLFPFYVGIPVGTINYFLAVRNKFSVEERVLKLDHNKFRLSAGTFVMQFGTESSKSE
                     TSGVGAAGGAAIGMMVGGPIGAVLGGALGAILGSFFGESLYDLRKKAYEQAQARLIEI
                     NNLVLSGIEQEIPKIYNELIQAIRDNYTKNKKDAVRLLLTYSGNESDTGQTVGIPKEE
                     HPLYKIDYGILIYTIVLILISILLWVKLHA"
     gene            1025..1363
                     /locus_tag="PK754_16415"
     CDS             1025..1363
                     /locus_tag="PK754_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11139.1"
                     /translation="MVTLKCRDAYSDLIGKQRAYEIIVNAIVPYVALYAQRRGDKQLH
                     DTVMMCMKTWASYEHNHIIGWMRKRLPGMPDRVNAEYAQGLIELHKKCRVRNCSDCRI
                     FQRMVYSDFI"
BASE COUNT          563 a          309 c          384 g          566 t
ORIGIN      
        1 agtaaatcat ctgaattcat acaattcaaa cggagatcta aaaaaaagat ttacatctga
       61 ttttgagctt ttgattaaaa acgggatgtc taatattata agctggtttg ataagaatat
      121 catgggcatt acatcagcgt atgattcttg gatcaacggg tttttgaagg acgcttatgc
      181 agtattaaaa aaatcggaac tagaaaaatg gtataaatcg gtctttttta ggaaatatgt
      241 tgttttctgt tttctattta gcttgatcag tataccattc ggtatttatc tcgatttcaa
      301 cctcctgctc ctattgtttc cgttctatgt tggaattcct gttggaacta ttaattattt
      361 tttagcggtc agaaataaat tcagcgttga agagagagtg ttgaaattag accacaacaa
      421 atttagactt tccgcaggta cttttgtaat gcaatttggc accgagtcct caaaatcgga
      481 aacttcagga gttggagcag ctggcggtgc agcaatcggc atgatggtag gaggtccgat
      541 tggcgctgtg ttaggaggtg ctttgggtgc tatactggga agcttctttg gagagtcgtt
      601 gtatgatctg cgaaagaagg cctatgagca agcccaagcc cgtctgattg aaataaataa
      661 tcttgtatta agtggtatag agcaagaaat tccaaaaatc tataatgaat tgattcaggc
      721 catcagagac aactatacga agaataagaa ggatgcggtt aggttgcttc tgacatatag
      781 cgggaatgag agcgacactg ggcaaactgt tggcattcca aaagaagagc acccgttata
      841 taaaattgat tatgggatat tgatatacac tattgtgtta atacttattt caattctatt
      901 gtgggttaaa ctacacgcat aaataacgaa cgaaaaagga atagaataat gcgtttagta
      961 gttcgtcatg ttactctttt ttgtagcgat tttgtgtcgt ttagcgatcc aagtttgcaa
     1021 aacaatggtt acattaaaat gtcgcgatgc gtattccgat ctgatcggaa agcaacgcgc
     1081 atacgagatc atcgttaatg cgatcgttcc gtacgtcgcg ctgtacgcac agcgccgagg
     1141 ggataaacaa ctgcatgata ccgtcatgat gtgtatgaaa acgtgggcat cgtacgaaca
     1201 caatcatatc atcggctgga tgaggaaacg cttaccgggc atgccggatc gggtcaatgc
     1261 cgaatatgcg caagggctga tcgaactgca taaaaaatgc cgtgtgcgca attgcagtga
     1321 ttgccgcata ttccaacgca tggtgtattc ggattttatt tagcgtgcag ttcatgtgac
     1381 gataaattta tatttattaa tatcgtataa tattgatcac tttggagaga agtataattt
     1441 catataaagc tgttttattt tttatttcgg cttaaactac caccgccttt ggcggtggta
     1501 gtttactaca tttttcttta catacgggcg aatattttac tttttttgtg ttcttcaatt
     1561 acataattta tttagttaat tgtaaacgat atttgctcta tctatgcaat aaatatttcc
     1621 aatcccttgt taactcattg aactacagaa cttgaggaat actctatgac aattgaacaa
     1681 gcactgaaga tactcgaaat aactaattcg aacccaaacc gcgaagagat aaaaactgca
     1741 tatcgcaggt tatctaaaaa ataccatcct gacgctacgg gatcaaagga tgcaacaaaa
     1801 tttctgatga tcaatgcagc tt
//
LOCUS       DAOQNQ010000468         1794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_47385_length_1794_cov_8.685451, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000468 DAOQNQ010000000
VERSION     DAOQNQ010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47385_length_1794_cov_8.685451"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1794
                     /locus_tag="PK754_16420"
     CDS             <1..>1794
                     /locus_tag="PK754_16420"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB11140.1"
                     /translation="RVTQYMHTMWTTDQGLPQNNITSMLQTRDGYIWCGTLDGLVRFD
                     GVRFEVFDKRNTPAIRNNAIITMFEDRSGGLWVGTIGGGLVHYKNGKFISYGLTEKFP
                     SAYILHMAEDEQGRLWFATRDGLVTRGDTSWITYTAKEGLTHTSVRSLFFDRKGRLWI
                     GSSVGLSLMENGVIRKIFAHELADKNVTSITSDSTDIIWITVLGKGVYRIKGDSIAHL
                     QAPKSIPHNFINAIARDKNGNRWLATDGGLYREHHGRFERFMDESNFPLDKVMCLLED
                     RDGNLWFGTNGSGLNRLRDEKFITYTTLEGLKDNSANVVFEDSRARIWVGTEAGVSII
                     DGDTIRNLGESSGLTNTKVRSFAEDTDGRIWVGTREGVFVWNEVRFESWNTRLKIPPL
                     VILSMLRARDGTMWIGTGGAGAVSYKPQNVQQYTSKQGLAGIVVIAMAEDSLGRMWFG
                     TNSGLSRFDGKNFVTYNSKNGMSSDIFLSLYVDKTNTLWAGTFGNGLNRLQNDSIAIF
                     TTADGLFNENMYSILEDGEGYLWMSCGKGVFRVAKEDFERYRQKIISVIPCKVFGRSD
                     GMRSSECNGTTQPAAWMTRSGWLLYPTGKGLA"
BASE COUNT          466 a          416 c          464 g          448 t
ORIGIN      
        1 cgcgtgacac aatacatgca tacgatgtgg acgaccgatc aggggctgcc acaaaataat
       61 attacctcta tgctccaaac gcgggacggc tatatctggt gtggcacgtt ggatgggctt
      121 gtacgatttg acggtgtacg ctttgaagtt tttgataagc gtaatacgcc ggccattcgc
      181 aataatgcaa tcatcacgat gttcgaagat cgctccggtg gtttgtgggt aggtaccata
      241 ggcggaggcc tagtgcatta taagaacgga aaatttattt cttacggatt aacggagaag
      301 tttcccagtg cgtatatctt gcatatggcc gaagacgaac aaggacgctt gtggttcgct
      361 acgcgcgacg gattggtcac tcgcggcgat acgtcgtgga tcacctatac ggcaaaagaa
      421 ggtttaacgc atacttccgt acgttcgttg ttttttgatc gtaaaggacg gctttggatc
      481 ggctcttcgg tcggactcag tttaatggaa aacggcgtga ttcgtaaaat tttcgcgcac
      541 gaacttgccg acaaaaacgt cacttcgatc acttccgaca gcacggatat catttggatt
      601 acggtactcg gcaaaggcgt gtatcgtatt aagggagata gcatcgcaca cttgcaagcg
      661 cccaaaagta ttcctcataa ttttatcaat gcgatcgctc gtgataaaaa cggtaatcgc
      721 tggttggcta ccgatggcgg tctctatcgt gaacatcacg gacgctttga acgatttatg
      781 gatgaatcga attttcctct cgacaaagtc atgtgtctgc tcgaagatcg cgacggcaat
      841 ttgtggtttg gcaccaacgg tagcgggctt aaccgcctgc gtgatgaaaa atttatcacc
      901 tatacaaccc ttgaaggtct taaagacaac agcgccaacg tcgtattcga agacagccgt
      961 gcccgcatct gggtcggtac cgaagccggt gtttcgatca ttgacggcga tacgatccgt
     1021 aatctcggcg aatccagcgg actgaccaac accaaagtgc gatcctttgc ggaagataca
     1081 gacggacgaa tatgggtcgg cacgcgcgaa ggtgtttttg tatggaatga ggtacgtttc
     1141 gaatcatgga atacgcgcct caaaattcct cccttggtca ttctctccat gctgcgcgcg
     1201 cgcgacggta cgatgtggat cggcacgggc ggcgctggtg cggtatcata caaacctcaa
     1261 aacgtacaac aatatacctc caaacaaggt ttagccggca tcgtcgtgat cgctatggcg
     1321 gaagactcgc tcgggcgaat gtggtttggc accaatagcg gactgagtcg cttcgacgga
     1381 aaaaactttg tcacatacaa ttccaaaaac ggtatgtcgt ctgatatttt tttatcccta
     1441 tatgtagaca aaaccaatac gctgtgggcg ggtacatttg gtaacggact caaccgttta
     1501 caaaacgact ccatcgccat atttacgacg gccgatggtt tattcaacga aaacatgtat
     1561 tccattctgg aagatggcga aggttatctg tggatgagct gcggtaaggg cgtgtttcgc
     1621 gtcgctaaag aagattttga acgataccgt cagaaaataa tatccgttat accatgcaaa
     1681 gtgtttggcc gcagcgacgg tatgcgcagc agcgaatgca atggcactac acaacccgcc
     1741 gcatggatga cccgcagcgg atggcttctg tatccgaccg gtaaaggtct tgct
//
LOCUS       DAOQNQ010000469         1792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_47488_length_1792_cov_4.966609, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000469 DAOQNQ010000000
VERSION     DAOQNQ010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47488_length_1792_cov_4.966609"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PK754_16425"
     CDS             <1..191
                     /locus_tag="PK754_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11141.1"
                     /translation="MKKNKKLNRRDTGHIQLSYYHVDCWEKRVDLQSMPINAAVGETM
                     REIPRKEKVKQTEKGVGL"
     gene            complement(441..938)
                     /locus_tag="PK754_16430"
     CDS             complement(441..938)
                     /locus_tag="PK754_16430"
                     /inference="COORDINATES: protein motif:HMM:NF023238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3332 domain-containing protein"
                     /protein_id="HNB11142.1"
                     /translation="MRKYTKVIALTLAVLMGSISVSSCYGKFALTRKLYTWNGSVTGN
                     KFVNNLVFWALLIIPVYSIAGFVDAILNTVEFWTGSNPMAMKEGEKEVKQFAHEGNTF
                     EVTATKNRLQIVQLTGEQAGASTVLVFRPESKSWYAETAEGAYKIASITDENNANFVN
                     PFAAK"
     gene            complement(995..>1792)
                     /locus_tag="PK754_16435"
     CDS             complement(995..>1792)
                     /locus_tag="PK754_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11143.1"
                     /translation="YLKNAALSGTAVINNPFWWSADEKFFNNALSIKVGVPVPKTVLL
                     PSNQHPPDTTALSMRNLAYPLDWEGIFKYVGFPAYFKPFAGGGWKNVYKLNNPEEFFK
                     AYNETGTLVMMLQEEIIFTEYFRCYCLGGKDVHVMQYDPRQPHHARYVKGPKTDAVKK
                     LEATVVDYVLRLNQALGYDFNTVELAVRDGIPYAIDFCNPAPDADLKSVGEDNFEWIV
                     EHAAQMAIERAKNHKPGQNNLTWGTFVKSFAADKPLGKTDTVKKKKK"
BASE COUNT          511 a          399 c          386 g          496 t
ORIGIN      
        1 atatgaaaaa gaataaaaaa ttaaaccgac gtgataccgg acacatccaa ttatcgtatt
       61 accatgtaga ttgttgggag aagcgcgttg acttgcaaag tatgccgatc aatgctgccg
      121 tcggcgaaac aatgcgcgag attccgcgta aagagaaagt gaaacaaaca gagaaaggag
      181 tagggctatg aaaccgacga atacgaagct gacgccaaaa ccgcagaaac gccctatctc
      241 caatgaaggg ccattgcggc caaatcaata acgctcctca ttacccacag gcaatagatg
      301 gatctatggg acagtccatc aaaattgcca aggcctcaga tataaacccc ggcattccaa
      361 gatgtgcggg gtttcttttt ttgaaataaa aaacccgctt tttagaagcg ggtttttttt
      421 aacggaagaa caattaaaaa ttattttgct gcaaaagggt ttacaaaatt cgcattattt
      481 tcatccgtga tgctggcgat tttgtatgca ccttcagcgg tctcagcata ccagctcttg
      541 ctttcggggc ggaatactaa tacggtggat gcgccggctt gttcgcctgt caattgaacg
      601 atttgaagac gatttttcgt ggccgtgact tcaaacgtat tgccttcatg tgcaaattgt
      661 ttgacctcct tttcgccttc cttcattgcc attggattgc ttccggtcca gaattctact
      721 gtgttgagta tcgcatcaac aaatccggca attgaatata cagggataat aagtaacgcc
      781 caaaacacaa ggttgtttac aaatttattt cccgtaacgg aaccgttcca tgtatagagt
      841 ttacgggtca atgcaaattt gccataacaa ctgctaacgc tgatagaacc catcaataca
      901 gccaaagtga gagcgatgac tttcgtgtac tttctcatgt tagtgactcc tgcgtgaggt
      961 tagaaatcgg ttaattaaat aagataaacg gaatttattt tttcttcttt tttacggtat
     1021 cagtttttcc cagaggttta tcggctgcaa aagatttcac aaaagtaccc caagtgagat
     1081 tgttttgccc cggtttgtga ttttttgccc gctcaatcgc catctgtgcc gcatgttcga
     1141 caatccattc gaaattatct tcgcctacgg atttgaggtc agcatccggc gccggattgc
     1201 aaaaatcaat cgcatagggg attccgtcgc gcacagccaa ttccacggta ttaaaatcat
     1261 atcccaaagc ctgattgagt cgtaacacat aatctacgac tgtggcttcg agttttttga
     1321 cggcatcagt tttcggacct ttgacatatc gtgcatgatg cggttgccgt ggatcgtatt
     1381 gcatgacatg cacatccttc ccgcccaaac agtaacaacg aaaatattcc gtaaaaataa
     1441 tttcctcttg aagcatcatg acaagcgtac cggtctcatt gtaagctttg aaaaattctt
     1501 caggattatt gagtttgtac acatttttcc aaccaccgcc ggcaaagggt ttgaaatagg
     1561 ccgggaaccc gacatatttg aaaatacctt cccaatccag aggataagcc aaattgcgca
     1621 tggacagtgc cgtcgtatcg ggtggatgct gattggaagg taaaagtacg gttttaggga
     1681 ccggtacgcc tactttgatc gaaagcgcat tattgaaaaa cttctcatct gcgctccacc
     1741 aaaaagggtt attgatcacg gccgtcccgc tgagcgcggc gtttttcagg ta
//
LOCUS       DAOQNQ010000470         1786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_47846_length_1786_cov_5.863663, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000470 DAOQNQ010000000
VERSION     DAOQNQ010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47846_length_1786_cov_5.863663"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PK754_16440"
     CDS             complement(<1..483)
                     /locus_tag="PK754_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11144.1"
                     /translation="MRTEAPLSAAKQKEGNFAKSKGIKMYKVLLLILVMVPLTLFGQI
                     PDGKKILDKAKKKNKEESKKEVKDKSKKSDDPSKSTATEKKAGEIPRIQQGSTAAKEN
                     SESKKSTHDDYQKLTVSADSDPFEVKTLKTYIVRMLECLTEVETDNSKQDQNIADHVF
                     S"
     gene            complement(510..1067)
                     /locus_tag="PK754_16445"
     CDS             complement(510..1067)
                     /locus_tag="PK754_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11145.1"
                     /translation="MKHIKHISKPILFLSLILTCATGACDDYEASLLDAAWKKFENQD
                     YDGAYAEFSALNGPNARIGLGWTLLRMDSIPEADRAFRIAAADSVLDGYAGIAITSWE
                     TGRYASAIYAANFVERWDPEYYFVHDPSVELNDLILHKAFCQYYLNDLTACNASIKKL
                     DPAWVTSTNPATILNKLEVLYDQMD"
     gene            complement(1087..>1786)
                     /locus_tag="PK754_16450"
     CDS             complement(1087..>1786)
                     /locus_tag="PK754_16450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB11146.1"
                     /translation="YVIADLGSGSTSVLRSGQAEMRILKSSARAEGYLILSEISSDND
                     LTNNGSNILQTCEILSTASQSWSSELSFNIAPAHRSDWNLTIPGFSTKHLKITRLDAT
                     GGWERRMIAHADENRVYTSINESGRYALVYDPSFSEIPKSLDLAQNYPNPFNPSTSIR
                     FGLANDGYVRIAVYNVLGQKIRNLISEQRTAGYHNVMWDGKNQKGSAVPSGIYIYRLE
                     TVDGILSKKMVLLK"
BASE COUNT          465 a          388 c          368 g          565 t
ORIGIN      
        1 cgaaaataca tgatcggcaa tattctgatc ctgtttgctg ttatccgttt cgacttccgt
       61 caaacattcc agcatgcgaa cgatatacgt ttttaacgtc ttcacttcga aaggatcgct
      121 atccgccgat accgtcaatt tttgataatc atcatgagtg cttttttttg attcggagtt
      181 ctcttttgca gcggtcgaac cttgttgtat tcgaggaatc tctcctgctt ttttttcagt
      241 tgcagtggat tttgacggat cgtctgattt ctttgatttg tccttaactt cttttttact
      301 ttcttctttg ttttttttct tagccttatc cagaattttt ttaccgtcag gtatttgtcc
      361 gaatagtgtc aaaggaacca tgacgagaat caataacagt actttataca tttttatgcc
      421 tttggattta gcgaagttac cctctttctg ctttgcagca gaaagagggg cttcggttct
      481 catgggagga gggttatttt tgttttattt caatccatct gatcatacaa cacttccagc
      541 ttattaagta tcgtagccgg attggtagat gtaacccaag ccggatccag tttttttata
      601 ctggcattgc acgcagtaag atcattcaaa tagtattggc aaaacgcctt gtggagtata
      661 agatcattga gttcaacgga tggatcatgg acaaaatagt attcgggatc ccagcgttcg
      721 acaaaattgg ctgcatagat agccgaagca tatcggcccg tttcccaact ggttatggca
      781 atccctgcgt atccgtctaa tacggaatcg gcagcggcaa tacggaaagc acgatccgct
      841 tcgggaatac tgtccatacg aagcagcgtc catcctaatc caatacgcgc attgggccca
      901 ttaagtgctg aaaattcagc ataagctccg tcataatctt gattttcaaa ttttttccag
      961 gcagcatcca gtaaacttgc ttcgtagtcg tcacaagcac ccgtggcaca ggtcaggatt
     1021 aatgacaaaa ataaaatcgg cttagaaata tgttttatat gtttcatgta attataggtt
     1081 tgatgtttac ttgagtaata ccattttttt ggaaagtatg ccgtcaaccg tctctaatcg
     1141 gtagatatag ataccgctcg gcaccgccga tcctttctga tttttcccgt cccacatcac
     1201 gttatgataa ccggctgttc gctgttcgga aatcaaattg cgaatctttt gaccaagtac
     1261 attatatacg gcgatgcgca catagccatc atttgcaagg ccaaatcgaa tgcttgtgct
     1321 cggattaaat gggttcggat aattctgtgc caaatctaac gacttaggta tctccgaaaa
     1381 ggatggatca tatacgagcg catatcgccc gctttcattg atgctggtat atactcggtt
     1441 ttcatcggcg tgcgcgatca tacgacgttc ccaaccaccc gtagcatcta atcgtgtaat
     1501 tttcaaatgt tttgtagaaa atccgggaat tgtaagattc caatcactcc gatgtgccgg
     1561 ggcgatatta aaacttaact ccgaagacca cgactgcgaa gcggttgata aaatttcgca
     1621 ggtttgcaaa atgtttgatc cgttattggt caaatcgttg tccgatgaaa tctctgaaag
     1681 gatcaaataa ccttcggcgc gcgctgatga tttcaggata cgcatttccg cttgaccgga
     1741 acgaagtaca cttgtactgc cggaacccag atcggctatg acatat
//
LOCUS       DAOQNQ010000471         1782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_48070_length_1782_cov_5.905038, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000471 DAOQNQ010000000
VERSION     DAOQNQ010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48070_length_1782_cov_5.905038"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PK754_16455"
     CDS             complement(<1..193)
                     /locus_tag="PK754_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020373911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB11147.1"
                     /translation="MPSLLSVRHLSAHFHVEDQLYKAVDDVSFDIASEETLCLVGESG
                     SGKSITALSVLDLLPYPGTI"
     gene            288..767
                     /locus_tag="PK754_16460"
     CDS             288..767
                     /locus_tag="PK754_16460"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNB11148.1"
                     /translation="MTEANTSPISLADLILPATIRLDLQGKTKQSVIDELIELISEAG
                     LVRDKSAVRQAVIDRERKLSTGLGHGIAIPHGKTSGVDKLVGAFGIKRDGIHFDSADG
                     APAKIFFLLVSPVTVTGPHIKALSGIARFLRIEKNRERLLQAATTDEVLALLGKPAV"
     gene            764..>1782
                     /locus_tag="PK754_16465"
     CDS             764..>1782
                     /locus_tag="PK754_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HNB11149.1"
                     /translation="MMQKLIIAAACFICILTPELTAQKLLIPMDLRQSDHLKAYGVAY
                     TALENKVPVEWLLNYRGGSFMIAYTETMANYCLARGVKTENLSAAQVAVVYATIENEN
                     MEIVYLEKAPHIAVYTPPDKKPWDDAVTMALEYAEIKYDKIFDLDVLQGKLAEYDWLH
                     LHHEDFTGQYGKFYSAYGRAAWYQQQQRDYETFAQQMGYSKVSKEKLGVVQAMKEFIA
                     KGGFMFAMCGATDSFDVALAAQKTDICDVVYDGDPVDMRANEKLDYGHCLAFQNFRVE
                     LDPYQYEFSSIDHPSSNGLRLKTPEEDYFTLFDFSAKYDPVPTMLTQNHVNVVKGFMG
                     QTTGYR"
BASE COUNT          495 a          386 c          437 g          464 t
ORIGIN      
        1 cgatggttcc cggatagggc agtaaatcca aaaccgataa tgctgtaata ctcttgccgc
       61 tgccggattc cccgaccaag cataatgttt cttccgatgc gatgtcaaaa ctgacatcgt
      121 ctacggcttt atagagttgg tcttcgacat gaaaatgagc cgataagtgg cggaccgaaa
      181 gtagtgaagg catatggatg gaggttaaat ttgaaagaaa taaagccaaa tatcctttga
      241 aaatcaacgg cgctgtgagt atctttcgcg cgcataaagg agatttgatg acagaagcga
      301 atacatcgcc gatttcattg gcggatttga ttttacccgc cacgattcgt cttgatttgc
      361 agggcaaaac caaacaaagt gtgattgacg aattaatcga attgatatcg gaggccgggc
      421 tggtgcggga taagtcggcg gttcggcaag ctgtaataga ccgcgaacgc aagctcagta
      481 ccggacttgg acacggtatc gctattccgc atggaaaaac aagcggggtt gataaactcg
      541 tcggcgcttt tggaataaaa cgcgacggga ttcattttga ttcggctgac ggcgccccgg
      601 caaaaatatt ttttctactc gtttctccgg taaccgttac aggaccgcac atcaaagccc
      661 tttccggtat tgctcgtttt ttacgcatcg aaaaaaatcg tgagcgcctt ttgcaagccg
      721 cgacaacgga tgaagtcttg gcgcttttgg gaaaacccgc cgtatgatgc aaaagctcat
      781 tattgctgcg gcatgtttta tatgtattct tacgcctgag cttaccgctc aaaaattact
      841 cattcccatg gatctgcgtc aatccgatca tctcaaagct tatggcgtcg catatacggc
      901 gttggaaaat aaagtacccg tcgaatggct tctcaactat cgcggcggat cttttatgat
      961 cgcctacacg gaaacgatgg ccaactactg tttagcgcgc ggcgtcaaaa cggaaaattt
     1021 atcagcggca caggttgctg tggtgtatgc gacgatcgaa aatgaaaaca tggaaatcgt
     1081 ctatctcgaa aaggcgcccc atattgccgt ttacacgccc ccggataaaa aaccttggga
     1141 cgacgcggtc actatggcgc tggaatacgc tgaaataaaa tacgataaga tttttgatct
     1201 tgatgttttg caaggaaaac ttgccgaata cgattggttg caccttcatc acgaagattt
     1261 tacaggacaa tacggcaaat tttattcagc gtacggccgt gcggcctggt accaacagca
     1321 acaacgtgat tatgaaacat ttgcgcagca gatgggatat agtaaagtct ccaaggaaaa
     1381 acttggtgtc gtacaggcca tgaaagaatt tatcgctaaa ggcggtttta tgtttgccat
     1441 gtgcggggcg acggatagtt ttgatgtggc tttggccgcg caaaaaaccg atatctgcga
     1501 tgtggtttac gatggtgatc cggtggatat gcgtgctaat gagaaattag actacggtca
     1561 ttgcctggca tttcaaaatt ttcgtgtcga actggatccg taccaatatg agttttccag
     1621 tattgatcac cccagcagta acggcttgcg gcttaaaaca ccggaagagg attattttac
     1681 gttgtttgat ttttcagcca aatacgaccc tgtaccgact atgctaacgc aaaatcatgt
     1741 caacgttgtc aaaggattta tgggtcaaac gacgggttac cg
//
LOCUS       DAOQNQ010000472         1766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_49004_length_1766_cov_3.991818, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000472 DAOQNQ010000000
VERSION     DAOQNQ010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49004_length_1766_cov_3.991818"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="PK754_16470"
     CDS             <1..914
                     /locus_tag="PK754_16470"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNB11150.1"
                     /translation="DSAVHKIRDRINYLQRNGVDMYTTTNKIAAISSDQNQSLVRQSS
                     SLSETSSTLEEMVRSSNQIADHASAVVGQAEDTERSAKKGVLRMEETVRMIKNVESKN
                     QLSLTEVIQLSESIQEIEQILRFINSVADETNLIALNATIEASSAGEHGKRFKVVANE
                     VRSLSDRVTSSVKNIQRMIDQVQEATTRLVTVAQETSEHTISSADMAQQTLTNLKEIL
                     EWAQRSSDAAKQIYISINQQRLANRQISFSFNDISADMRALAASSERYAETVDALKKF
                     SNNMEAVVKYFVKQQHSEKPDHEKNTT"
     gene            895..>1766
                     /locus_tag="PK754_16475"
     CDS             895..>1766
                     /locus_tag="PK754_16475"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNB11151.1"
                     /translation="MKKTLREILNNNALVRNVYGSTSLTVTGLCTDSRFAQKDNIFYA
                     LRGTQKDGNKFIAHAIQKGASVIVSDELYAKVQFPPKTRVTYVQVENLTQFMAESSAS
                     FYGTTNAPMAFIGITGTNGKTTTAKFINETLKALGHKTVFLGTTGFEVCGRIIPTDFT
                     TPPAPMLHKYLREGMDKGATHVVMEVSSHALKLKRVWGLRFDAAVFTNLTHEHRELHV
                     SMDDYFSTKALLFGMLKPVGFAVINGDDDFSGGMRKSVPSGHRIVEYGKKNSATLRLE
                     KIKFNPDSRLQSIA"
BASE COUNT          529 a          449 c          373 g          415 t
ORIGIN      
        1 tggactccgc cgtgcataaa atccgggacc gtattaatta tctgcaacgt aacggtgtgg
       61 atatgtatac gacgaccaat aaaatagccg cgatctcttc cgatcaaaat caatcgttgg
      121 tacgccaatc ttcttcactg tcagaaacct cctcgacatt ggaagaaatg gttcgctcat
      181 ccaatcagat cgccgatcat gcgagcgccg tagtcggtca ggccgaagat accgagcgat
      241 cggctaaaaa aggcgttctt cgcatggaag aaaccgtgcg catgatcaaa aatgtggaat
      301 ccaaaaatca actcagcctc actgaagtca tccaactcag cgaaagtatt caggaaatag
      361 agcaaatttt acgtttcatt aattcggtag ccgacgagac caatctcatc gcacttaacg
      421 ccacgatcga agccagttcc gccggcgaac acggcaaacg attcaaagtc gtagccaatg
      481 aagttcgttc gctttcggat cgcgttacaa gctccgttaa aaatattcaa cgtatgattg
      541 atcaggtgca ggaggccaca acgcgcttag ttaccgttgc ccaagaaact tccgagcata
      601 cgatcagcag cgccgacatg gcccaacaaa cactgacgaa cctcaaagaa attttagaat
      661 gggcgcaacg ctcctccgat gcggccaaac aaatatacat ctcgatcaat caacaacgtt
      721 tggccaatcg gcagatatcc ttttcattca acgatatttc tgccgacatg cgcgcattag
      781 cggcctccag cgaacgttac gcggaaaccg tagatgcact caaaaaattc tccaacaata
      841 tggaagcggt tgtaaaatac ttcgtcaaac aacaacattc tgaaaaaccg gatcatgaaa
      901 aaaacactac gtgagattct taataataac gctttagtac gcaatgttta tggcagcacg
      961 agcctcactg ttaccggact ttgtaccgat tcacgttttg cacaaaaaga taacattttt
     1021 tacgccctcc gtggtacgca aaaagacggt aacaaattca tagcgcacgc catacaaaaa
     1081 ggcgcgtccg tcatcgtctc ggacgaatta tacgccaaag tacaattccc tcccaaaacc
     1141 cgcgtgactt acgtacaggt ggaaaaccta actcagttta tggcggagtc ctccgcttct
     1201 ttttacggca cgaccaatgc gcctatggct tttatcggta ttacaggcac caacggaaaa
     1261 acgacaacgg ccaaatttat taatgaaacc cttaaagcat tgggccacaa aaccgtgttt
     1321 ctaggtacga cggggtttga agtgtgcggc cgaatcatac caacggattt tacaacgccg
     1381 cctgccccga tgctccacaa atatctacgc gaaggcatgg ataaaggcgc tacgcatgtt
     1441 gtcatggaag tttcttcgca cgcactcaag ctcaaacgtg tatggggcct tcgttttgat
     1501 gcggcggtat ttaccaatct gacgcacgaa catcgtgaac tgcacgtttc gatggatgat
     1561 tatttttcga ccaaggcttt actctttggc atgctcaaac cggtcggttt tgccgtgatc
     1621 aacggagatg atgattttag cggaggtatg cgcaaatcgg ttccctccgg tcatcgcatc
     1681 gtagaatatg gcaaaaaaaa cagtgccact ttaagactgg aaaaaataaa atttaatccc
     1741 gattcacgcc ttcaaagcat cgcctt
//
LOCUS       DAOQNQ010000473         1757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_49512_length_1757_cov_5.111633, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000473 DAOQNQ010000000
VERSION     DAOQNQ010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49512_length_1757_cov_5.111633"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PK754_16480"
     CDS             complement(<1..276)
                     /locus_tag="PK754_16480"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HNB11152.1"
                     /translation="MEKVGGSKLIKDIEIGSQTDNIIVVRKKEDEFVVFSAICRHKKC
                     NVKYKNEKSLFVCPCHGSTYDMDGKVQKGPSTTDILKYKSERKGDKLI"
     gene            484..1449
                     /locus_tag="PK754_16485"
     CDS             484..1449
                     /locus_tag="PK754_16485"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HNB11153.1"
                     /translation="MTLDDFVTKKKPHWYKLHALVDTYERDKNLPHQDLTEMIRLYRA
                     ACADYAFARLTFPYERIVSELHQLISRSHNTIYGRTKITRYHWSSYFLIDIPTTILRH
                     RRLIFLSALVFVLGGLFAFAGSLANNDLPRHLLGDAYVSKTESNIRSDDPFAIYKSEQ
                     SPAMSGFLMTNNIKVTFLTFAFGLFAGIGTLYVLFHNGLIIGAFFYIFFKHGLLFSSF
                     LTIMMHGTLELFSVFIAGGAGLCVGRALVFPGNFPRITALRNASREALTLIIGIVPML
                     IIAGIIEGFITRLSLPDEMKIIFIGANLIVLGWYLMRSKAMDKVN"
     gene            complement(1556..>1757)
                     /locus_tag="PK754_16490"
     CDS             complement(1556..>1757)
                     /locus_tag="PK754_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNB11154.1"
                     /translation="SAKIIMDKMSGRSKGFGFVEMATEEEASKAIEMFHNYSFMERNL
                     TVNVAKPMEKKSFGDRSRSNRY"
BASE COUNT          467 a          356 c          322 g          612 t
ORIGIN      
        1 gataagttta tcgccttttc tttccgattt gtattttaga atgtcggttg tggacggtcc
       61 tttttgcact ttcccgtcca tatcgtatgt actaccgtga caagggcata caaaaagcga
      121 cttttcgttt ttgtatttta cattacattt tttgtgacga caaattgcag aaaaaaccac
      181 aaactcatct tcctttttac gaacaacaat tatattgtcc gtttgtgagc ctatttcgat
      241 atcttttata agctttgacc cgccgacttt ttccaattcg ggatagttct tgatcaagat
      301 ttcgaatttt tcattttcgc ccaataatgt ttttttagcg gtaaatccca ttccaaaaat
      361 tcccgcgcaa acagttgcca tgcgtagcaa aaattgtctt ctgatcattt tactttcttt
      421 acgttttatg ataaaattca gatattgtaa tatatagttt atcgaagagg ctcacaagtt
      481 tttatgacat tagatgattt cgtgactaaa aagaaaccgc actggtacaa gcttcacgca
      541 ttagtagata cgtatgagcg tgataaaaat ttaccacatc aggatttgac ggaaatgatt
      601 cgcctttacc gtgccgcttg tgctgattat gcatttgccc ggttgacatt tccttatgaa
      661 cgtatcgtta gcgagttgca tcagctcatc agtcgctctc ataacacgat ctacggaaga
      721 acaaaaataa cacgctatca ctggtcatca tattttttaa tagatattcc gaccactatt
      781 ttacgtcacc gacgtctgat ctttctttca gctttggttt ttgtgcttgg cggattattt
      841 gcgtttgccg gttctttggc taataacgac ttgccaaggc atttacttgg tgatgcctat
      901 gtcagcaaaa cagaatccaa tattcgttca gacgatccct ttgcaatata taaatccgaa
      961 caaagtcccg cgatgtccgg gtttttgatg actaataata taaaagtaac atttttaaca
     1021 tttgcatttg gcctttttgc cggcatcggt acgctctatg ttttatttca caacggactg
     1081 atcattggcg cattctttta tatatttttt aagcatggtc tgctgttctc ttcgtttctt
     1141 acgatcatga tgcacggaac attggaatta ttcagtgttt ttattgccgg cggggccggt
     1201 ttgtgcgtcg gacgtgcttt ggtttttccc ggaaattttc cacgcataac agctttacgc
     1261 aacgcatccc gcgaagccct cacgctgatc atcggcattg ttcccatgct tattatcgca
     1321 ggtattatcg aaggctttat tacaaggctt tctttaccgg acgagatgaa aattattttt
     1381 attggtgcga atttaattgt attaggttgg tatctgatgc gttccaaagc aatggataaa
     1441 gtaaactaat taatactcta ttgaattgaa ttatttaaac aaaaaaacct cagctatttt
     1501 tatagctgag gtcaaaatta acttcggttc aacccgaagt gattttcaat atcgattaat
     1561 agcggtttga gcgtgaacga tcgccaaacg atttcttttc catcggttta gcaacattca
     1621 cagtcagatt gcgttccata aacgaatagt tgtggaacat ttcgatcgct tttgaagctt
     1681 cttcttcggt ggccatttcg acaaaaccaa aacctttgga acgaccggac attttgtcca
     1741 tgatgatctt agcagac
//
LOCUS       DAOQNQ010000474         1755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_49612_length_1755_cov_5.350000, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000474 DAOQNQ010000000
VERSION     DAOQNQ010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49612_length_1755_cov_5.350000"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..>1755
                     /locus_tag="PK754_16495"
     CDS             256..>1755
                     /locus_tag="PK754_16495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB11155.1"
                     /translation="MKILILDAVASDADALEKRLRRRSITVKVRRAHIKAVFLRELRE
                     FQPDIILADYTLPDLNGIDALTLAGDLAPKAPFIFVTAHNGEAVAVRCMKSGAFDYVL
                     KDKIEDLDHVLERAETFIQNNAVPITAHFEMAPEQFRRIAESTRDLITVIDIEGRRIY
                     TSPSYGDYLDTHQVEQGSSFFDEIVAEDLPKVKQTFYEVIHTGNAMVVEHRIMAKDRK
                     LMFVEAQWNPIFDNSEKPVHIQIASRIITRRKRAEDALREHVRQYRAMVEHIADAISL
                     INPYGIVLFTSRSTHRVTGYNISEFVSQNFFDFIHPQDRAEALEYFQQVQAKEGMAPY
                     TEMRIRHKDGNYIWIEGLAKNMLHDPNVNAIVFNYRDITEWLRSEAALREKDELFRAL
                     IENNSDAITLMDAEGQIKFSVHSVLGYDDAEAVGQNIFDSIHPEDQDRIAEVFEQLIE
                     TPSTSLVAEYRAMHKDGSWRWNECTAKNLLKEPHVRAIVINARDVTDRKL"
BASE COUNT          482 a          436 c          400 g          437 t
ORIGIN      
        1 ttttattaag gccatgagcg ctttcaaaaa agcttacgaa atcaatccgt cctataaaag
       61 cgcatacaaa aacttccgat tggtacaaaa cgacggacgg gaattcctca atctcttgcg
      121 ggccatactc aagtaagcat cgtgctgtgg cattaacggt atttcagtgg tagttttata
      181 ttgcctaagt acgccctttg cgcgtatatt caccggcgat acaatggttc caattaatcg
      241 tttaaacacc gtctaatgaa aattctgatt ttagatgccg tggcgtcgga cgccgatgcc
      301 ttggaaaaac gtctgcgccg tcgttcgatc acggtcaaag tgcgtcgtgc gcatatcaaa
      361 gcggtgttcc ttcgcgaatt gcgagaattt caacccgaca ttattttagc cgattataca
      421 ctccccgatc tcaacggtat tgacgccctt acgctggccg gcgatttagc tcctaaagct
      481 ccttttattt ttgtcacggc gcacaatggt gaagccgtgg ccgttcgctg tatgaagtcc
      541 ggcgcttttg actatgtgct caaagataaa atcgaagacc tcgatcacgt gcttgagcgg
      601 gccgaaactt tcatccaaaa taacgccgtt cctatcacag cacattttga aatggcgccg
      661 gaacagtttc gtcgcatcgc cgaaagtaca agggatctga tcacggtgat agacatcgaa
      721 ggacgacgca tctacacgag tccatcgtat ggcgattatc tggacacaca tcaggtggaa
      781 caaggctcat ctttttttga tgaaatcgtg gcggaagatt tacctaaggt caaacaaact
      841 ttttatgaag tgattcatac cggcaatgcc atggtcgtcg aacatcgtat catggctaaa
      901 gaccggaaac tgatgtttgt cgaagcgcaa tggaatccga tatttgataa ttccgaaaaa
      961 ccggtgcaca tacagatcgc ctcgcgcatt atcacacgcc gcaaacgcgc cgaagacgcc
     1021 ttacgcgagc atgtgcgcca ataccgcgcc atggtggaac acatcgccga cgcgatttcg
     1081 ctgatcaatc cctacggtat cgtgcttttt accagccgtt caacgcaccg tgtcacgggc
     1141 tataatatca gcgaatttgt cagtcagaat ttttttgatt ttattcatcc gcaagatcgc
     1201 gccgaggcat tggaatattt tcaacaagtc caggccaaag aaggtatggc gccttatacc
     1261 gaaatgcgca ttcgccacaa agacggcaac tacatttgga tcgaagggct tgctaaaaac
     1321 atgctacacg accccaatgt caatgcgatc gtttttaatt accgtgacat caccgaatgg
     1381 ctgcgttcgg aagcggcgtt gcgcgaaaaa gacgaattat tccgcgcctt gatcgaaaat
     1441 aattccgatg cgatcacgct tatggacgcc gaaggacaga tcaagttcag cgtgcattcc
     1501 gttttaggat acgacgacgc cgaagccgtc gggcaaaata tttttgattc catacacccc
     1561 gaagatcaag accgtatcgc cgaagtgttt gaacaattaa ttgaaacacc gagcacatcc
     1621 cttgtcgccg aatatcgcgc catgcacaaa gacggctcct ggcgatggaa tgaatgtacg
     1681 gcaaaaaatc ttttgaaaga accgcatgtg cgtgcgatcg tcatcaatgc ccgcgatgtg
     1741 accgatcgta aactt
//
LOCUS       DAOQNQ010000475         1747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_50094_length_1747_cov_3.586288, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000475 DAOQNQ010000000
VERSION     DAOQNQ010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50094_length_1747_cov_3.586288"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1747)
                     /locus_tag="PK754_16500"
     CDS             complement(<1..>1747)
                     /locus_tag="PK754_16500"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB11156.1"
                     /translation="SYGRLWIGTTEGGINLMTGDTFLNFRNTPNQPSLFTDEYIYDIY
                     QSSDGIVWACTYSNGVTQISGMEPRFVHYKKNPLDSHSLIHNVVRSFTESGDGSIWIS
                     TDDGISRWDRNTDRFEHLRFIPDNPNALSDRQIHRVYEDRSGKIWIGTHQSGLQCYDP
                     VRKTMKSFRRSTTQGQFAAIRVYDILEDENGYIWLATYRSGLIRYDPQNDQFKTYGLK
                     KAGGQNIRKEMLTVLHRDNAGKLWIGTQQRGLLCFDRQSEQFTTFSKNTAEAFTISDN
                     HVYSIYQDKSGYLWIGTANGLNRFDPLTQKFQRITRKDGLPNNVIYGIIGDEHDNLWL
                     SSNRGIIRMQTKSDTSRVFTVYTSDDGLQADQFEPNACLRTRDGEFLFGGVNGFNIFH
                     PRAIVPHGQAPRLALTDFRLFDKPTKLDTSIPYKHDLHLNYDQNFFSFSFSALNFFVP
                     EKNQYAYKLDGIDENWNFVTGKNRIVKYTDIDPGNYTLLVRASNHDQIWNNDGLRFPI
                     VIRPPYWETWWFRTILFAALIGVLIFAYSARVRNIEAMNRRLEGIVIERTAALNQKKD
                     ELQHAHDELEKRVEER"
BASE COUNT          474 a          373 c          387 g          513 t
ORIGIN      
        1 cgttcttcga ccctcttttc caattcgtca tgcgcatgct gcaattcatc tttcttctga
       61 tttaaagctg ccgtgcgttc gattacgata ccttcaagtc tgcggttcat tgcttcaata
      121 ttgcgtacac gagcggaata agcgaatatt aaaactccaa tcagcgcggc aaataagatc
      181 gttcggaacc accaagtttc ccaatacggt gggcggatca caatcggaaa acgaagaccg
      241 tcgttattcc aaatctgatc gtgattggaa gcgcggacca ggagcgtgta atttcccgga
      301 tcaatgtccg tatattttac gatacgattt tttcctgtta caaaattcca attttcatca
      361 ataccatcca gtttgtaggc gtattgattt ttttcgggga caaaaaaatt gagtgcggaa
      421 aatgaaaaac taaaaaaatt ctgatcataa tttaaatgaa gatcgtgttt gtaaggaata
      481 cttgtatcca atttagtggg tttgtcaaaa agcctgaaat ccgtaagcgc tagtcgcggt
      541 gcttgtccgt gaggtacgat agcgcgcgga tgaaagatat taaatccatt aacaccgcca
      601 aaaagaaatt cgccgtcacg cgtgcgaagg caagcattcg gttcaaattg atcagcctgt
      661 aaaccgtcat cggatgtata aacagtaaaa acgcgggaag tatcggattt tgtttgcatc
      721 cgaataatac cgcgattact gctcagccac agattgtcat gttcatcgcc gataataccg
      781 taaatgacat tgttaggcaa accgtcttta cgggtgatgc gttgaaattt ctgtgtaagt
      841 ggatcaaagc gatttaaccc gttggccgtg ccgatccata gataacctga tttatcctga
      901 tagatgctat atacgtggtt atctgaaatc gtgaaggctt ccgccgtgtt tttggagaat
      961 gtcgtaaact gttcggattg ccgatcaaaa cacagcagtc ctctttgttg tgtaccgatc
     1021 cataatttcc cggcgttgtc tctatggagt acagttaaca tttctttacg gatattctgg
     1081 ccgcctgctt ttttaagccc ataggtctta aattgatcgt tttgtggatc atatcgaata
     1141 agtccgctgc gataggttgc aagccaaata tatccatttt cgtcttcgag aatgtcgtat
     1201 acgcgaatgg cagcaaattg accttgtgta gtcgagcgcc gaaaggactt cattgtttta
     1261 cgtaccggat cataacattg caagccgctc tgatgcgtac cgatccatat tttacctgaa
     1321 cgatcttcgt aaacgcggtg aatctggcgg tcacttaaag cgtttggatt atccggtata
     1381 aaacgcaagt gttcaaagcg atctgtattt ctgtcccatc gtgagatgcc gtcatcggtg
     1441 ctgatccaaa tcgaaccgtc accactctcc gtgaaactgc gtactacatt atgtataagg
     1501 ctgtgcgagt ccaacgggtt ttttttgtag tgaacaaaac gcggttccat tccgctgatt
     1561 tgtgttactc cattggaata cgtacaagcc catacaatgc cgtcggaaga ttggtaaata
     1621 tcgtagatat actcatcggt aaaaagagaa ggctgattgg gcgtattgcg gaaatttaga
     1681 aacgtgtcgc cggtcatcag attgatgccg ccttcggtgg ttcctatcca taatctgcca
     1741 tacgagt
//
LOCUS       DAOQNQ010000476         1734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_50839_length_1734_cov_3.530077, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000476 DAOQNQ010000000
VERSION     DAOQNQ010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50839_length_1734_cov_3.530077"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PK754_16505"
     CDS             <1..287
                     /locus_tag="PK754_16505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11157.1"
                     /translation="QNWSGLFVSLDARGYKGLNDFMYDRPIKGTIEWKKQEVVIQVPV
                     DAERIFYGVWLEGKGKIWIDDFVIEIVGEETPITAGRRILKRPRNLNLEE"
     gene            296..1387
                     /locus_tag="PK754_16510"
     CDS             296..1387
                     /locus_tag="PK754_16510"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB11158.1"
                     /translation="MSTEGKIKKLDLMHWYTERRHRVLLVDDEMRIAQEVQDMLINDG
                     IEVQIAPNGKSALEMLPKFQPDVVLTETVIRYVNGFELCRQIKENKATQNIPVIIVTG
                     LSRPADKIRGIEAGANDFVTKPFDAMELKTRVRSAIRMKELNANLEDFDEVILAFSRA
                     VEARDPYTRGHSERVGKYSIKLGVALGVPKGFQDILYKGAILHDIGKIGVRDSVLLKK
                     GKLTAEEYDEIKKHPDIGVKICGPLKSSQPLINIIAYHHERMDGKGYPYKIGGKDIPI
                     EARIVAIADTFDALTSARPYREPLTAIQACHLLEEGIGKHFDEELLPEFIDIALRGEL
                     QEVMQDPVQASPPVQNLTDDFVKELDFLM"
BASE COUNT          473 a          361 c          438 g          462 t
ORIGIN      
        1 ttcaaaactg gagcggcctt tttgttagtt tggatgcgcg tggttacaaa ggtcttaatg
       61 attttatgta cgaccgcccg atcaaaggaa cgatcgaatg gaaaaaacaa gaagtcgtta
      121 ttcaggtgcc ggtcgatgcc gaacgtattt tttacggggt gtggctggaa ggtaaaggaa
      181 aaatttggat cgatgatttt gtgatcgaaa ttgtcggtga agaaacgccg ataacagccg
      241 gacgtcgcat actaaaacgt ccgcgtaatc tcaatttgga ggaataaacc gctgtatgag
      301 cacggaagga aaaataaaaa aattagattt aatgcattgg tacaccgaac gtcgccatcg
      361 agttctactt gtggatgacg agatgcgtat tgcgcaagaa gtgcaggata tgttgatcaa
      421 tgacggcatc gaagtgcaga tcgcgcccaa cggtaaatcg gctcttgaga tgttgcctaa
      481 atttcagccg gatgttgtat tgacggagac ggtgattcgg tatgtcaacg gatttgaact
      541 ttgccgccaa atcaaagaaa ataaagccac acaaaatatt cctgtgatta tcgtaaccgg
      601 cctttcacgt ccggcggaca agatccgtgg tatcgaagcg ggagctaatg attttgtgac
      661 caaacctttt gacgcgatgg agcttaagac gcgcgttcgt tcggccatcc gcatgaaaga
      721 gttaaatgca aatctcgaag attttgatga agtgattctc gcattttcac gggctgtaga
      781 agcgcgcgat ccgtatacgc gcggtcactc cgaaagagtc ggaaaatatt cgatcaagct
      841 gggtgttgcg ctcggcgtac ccaaagggtt tcaggatatt ttgtacaaag gcgccatact
      901 gcatgacatc ggtaaaatcg gcgtacgtga ttcggtactt ctcaaaaaag gaaagttgac
      961 cgctgaagaa tatgatgaaa taaaaaaaca tccggatatc ggagtaaaaa tttgcggtcc
     1021 tctgaagtcc agccaaccgc tgatcaatat catagcttat catcatgagc gcatggatgg
     1081 caaaggttat ccgtacaaaa tcggagggaa agatattccc atcgaagcgc gtatcgttgc
     1141 tattgccgat acctttgacg cgctgaccag tgcgcgcccg taccgcgaac ctttgaccgc
     1201 cattcaggct tgccatttgc ttgaggaggg gattggtaaa cattttgatg aagagctgct
     1261 cccggaattc attgatattg cgctacgcgg tgagttgcag gaagtgatgc aggatcccgt
     1321 acaggcgtca ccgccggttc aaaatctgac ggatgatttt gtaaaagagc tcgacttttt
     1381 gatgtgacac gaaaccatct ttcttaatac tcgtaccact atccaagttt tatccacatt
     1441 ttaaatccgg gttgtatgtc tgacgaagaa caaaacgtac cgtcgccgtt tcaatcatgg
     1501 cgcggttggt acggattgac cgtaatgatg ttggtcgttt atattttcat cttctatatc
     1561 atacggattg cctaccaatg accgccctcg attggacggt actcgccgtt tctcttattt
     1621 ttattattgc gtacggtatc tataaaagcc gcggtgtggc tacaatggat tcgtatgtcc
     1681 tttccaatcg tgacatgcgg tggtggacgg ttgcgatttc gatcatggcg acac
//
LOCUS       DAOQNQ010000477         1723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_51485_length_1723_cov_4.485612, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000477 DAOQNQ010000000
VERSION     DAOQNQ010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51485_length_1723_cov_4.485612"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..>1723
                     /gene="ggt"
                     /locus_tag="PK754_16515"
     CDS             124..>1723
                     /gene="ggt"
                     /locus_tag="PK754_16515"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNB11159.1"
                     /translation="MFLRIFFLTALIIFPSCQNSHQTSRISSSVTAQNGVVVCVDQNA
                     ARVGLEILQKGGHAVDAAIATAFALAVTYPQAGNIGGGGFMMVRTSDGNVKAIDYREK
                     APYQAHARMFLNADGSEDTITSNYGYKVAGVPGTVRGLEKAWKLYGKLPWSELINPAI
                     RLAEEGFILEAAEARRLSEFAPALKRFDETAHMFFKPDGSPYAEGELLIQKELAATLR
                     AIADEGADVFYRGKIATQIADDMKAHGGLITHADLAAYEAVVREPIRGNYRGYTIYSM
                     PPPSSGGVTLIEMLNILENFTLHDDTISTRRVIETMRRSFLDRLVYLGDPDFSDIPVS
                     ALTDKALARKLASWIDSAHINAKMPALAMKQEPTETTHFSVIDKNGNCVTNTYTLEDN
                     FGSKAVVTGLGFLLNNEMHDFDVFPDSNRFPGHGQKPNRIEPHKRMLSSMTPVIMLKE
                     DKPFLITGSPGGKTIINTVLQIIIGMIDYGDSLRQAVDRPRLSHTWSPNVLSYEKDKL
                     DETRRAFLLRNGYILSEKPFIGDAH"
BASE COUNT          440 a          377 c          444 g          462 t
ORIGIN      
        1 gcgtaagctt cgttttagct acttaaagcc gcgaagcgga gcttacacgt tatacaatcg
       61 ttgcttgtgg ttttttatca tattcgctaa attgcttcag caatttagtt tgagattggg
      121 attatgtttc ttcggatttt ttttcttacc gcgctgataa tttttccctc ctgccagaat
      181 tcgcaccaaa catcgcgtat atcatcttcc gtaaccgcac aaaacggtgt cgtggtctgt
      241 gttgatcaga atgcggcgcg tgtcggcttg gaaattttac aaaaaggcgg tcatgccgtg
      301 gacgcagcta ttgctacggc ttttgctttg gccgtgacgt atccgcaagc cggcaatatc
      361 ggcggtggcg gatttatgat ggtgcgaaca tccgacggaa atgtgaaagc catagactac
      421 cgtgaaaaag cgccgtatca agcgcatgca cgtatgtttt tgaatgcgga tggcagcgag
      481 gatacgatca ccagtaatta tggatacaaa gttgccggtg tgccgggaac agttcgcgga
      541 cttgaaaaag catggaaatt gtacggtaag ttgccctggt cggaactgat aaacccggcg
      601 atacgtttgg cggaagaagg gtttatcctc gaagcggcag aagcacggcg actaagtgaa
      661 tttgcgccgg cgctcaaacg gtttgatgaa acggcgcaca tgttttttaa acccgacgga
      721 tcgccttatg ccgaaggcga acttttgatc caaaaagaac ttgcggcgac gttgcgtgct
      781 atagccgatg aaggcgcgga tgttttttat cggggtaaaa tcgccacaca aatagccgat
      841 gacatgaaag cgcatggcgg actgatcacg catgcggatc tagcggccta cgaagcggtg
      901 gtgcgggaac ctatacgagg gaattatcgc ggttatacca tatacagcat gccgccgccg
      961 tcttcgggcg gagtgacatt gatcgagatg ttgaacatac tcgaaaattt tacactgcat
     1021 gacgatacga tatcaacgcg gcgcgttatc gaaacgatgc gtcgttcctt tttagaccgg
     1081 ttagtttatc tcggtgatcc tgatttttca gatattccgg tttccgctct gacggataaa
     1141 gcgctggctc gcaagttggc ttcatggatt gattcggcgc atatcaatgc taaaatgccg
     1201 gccttagcca tgaaacagga accgaccgag actactcatt tttcggtcat tgataaaaac
     1261 ggtaattgcg ttaccaatac ctatactctc gaagataatt ttggttccaa agctgtggtg
     1321 accggtttag gatttttgct caataatgag atgcatgatt ttgatgtttt tccggacagt
     1381 aatcgctttc ccggtcatgg gcaaaaaccc aatcgtatcg aaccgcataa gcgcatgttg
     1441 agttcgatga ctccggtgat catgctcaaa gaggataaac cgtttttgat cacgggttcg
     1501 cccggcggta aaacaatcat caataccgta ctgcaaatca tcatcggcat gatagattat
     1561 ggggattcct tgcggcaggc ggtggatcgc ccgcggttga gtcatacgtg gtcaccgaat
     1621 gtgttatcct acgaaaaaga taaactggac gaaacgcggc gcgctttttt attacgaaac
     1681 ggctatatat tatcagagaa gccatttatc ggtgatgccc att
//
LOCUS       DAOQNQ010000478         1712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_52212_length_1712_cov_1.599879, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000478 DAOQNQ010000000
VERSION     DAOQNQ010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52212_length_1712_cov_1.599879"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..1295)
                     /locus_tag="PK754_16520"
     CDS             complement(42..1295)
                     /locus_tag="PK754_16520"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNB11160.1"
                     /translation="MNNNNSVYFIVFFGDKPPEYLENYVARYAAGIGLTCRLEKKNNA
                     KITFVKASLGTIQNPSDILFSDTHPSGDFHQNSHHLHWAVASEEITIYCAPWIVKQIY
                     YVRCGNGWLISDDLRLMIGFASSEIDEVALYACLQFNSVPAPLTLFRSIRRTRPGCVY
                     RFSSKTVIEDTPYRIPPLENKTYDEAHQDTQKTLSDTIQSIPDRSVLFFSGGVDSALI
                     MAYAGEIQKKIEPVFLSFGLDDPELSVVSAISTHLRMPIHVAEFTMAQAETQMMRIGL
                     TYSFPFCDYATLPSMVLAEYVAQKFPGHSAIDGTGADGLHGGWTAYTKWQRRYRSPYL
                     VRKMKSWMYRSLSVWRYDTTWSATLAKHKLSAEWPLPVAWYLARNPFNGILYTIPDSV
                     KKEVLQYILDQPMYHEQNLLAKPVV"
     gene            complement(1292..>1712)
                     /locus_tag="PK754_16525"
     CDS             complement(1292..>1712)
                     /locus_tag="PK754_16525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB11161.1"
                     /translation="AIQQEHVSDCVTMAGELNNTHVLREIASADLFVMPSIITSNGDR
                     DGIPNVIIEAMFLKTPMVATDVSGIPEVVKHRETGLLCEPANALALADAMAESLIQSD
                     QTRACTASAYQFVNNYFDMEKNVHRLYEHIVSSEKSV"
BASE COUNT          494 a          414 c          341 g          463 t
ORIGIN      
        1 gccaaattca tgccaacttc ttttattaaa tcataattaa ttcacacaac gggtttagcc
       61 aataaatttt gctcatgata cattggttga tccaaaatat attgcaaaac ttcttttttt
      121 accgaatccg gtatggtata aagaatacca ttaaacggat tacgtgccaa gtaccaagcc
      181 acaggcaaag gccattccgc cgacaacttg tgtttagcca acgtagcgct ccatgtcgtg
      241 tcataacgcc aaaccgacaa actccggtac atccatgact tcattttacg cacaagatac
      301 ggcgagcgat agcgccgttg ccattttgta tatgccgtcc agccgccgtg caatccgtct
      361 gcaccggtac catcaatagc gctatgtcct ggaaatttct gagcaacgta ttcagctaat
      421 accatagaag gtaatgttgc gtaatcacag aacggaaatg aatacgtaag accgatacgc
      481 atcatttggg tttcggcttg cgccatcgta aactctgcga catgaatcgg cattctcaaa
      541 tgcgtactta tggcacttac taccgaaagc tcaggatcgt ccagcccaaa actcaaaaat
      601 acaggttcga tttttttttg tatctctccg gcatacgcca tgattaaagc cgaatccacg
      661 ccgccgctaa aaaataaaac cgacctatcc ggtatagatt gaatcgtgtc cgagagcgtc
      721 ttttgcgtat cctgatgcgc ttcatcatat gtcttatttt ccaacggcgg aatacggtaa
      781 ggggtatctt cgatcaccgt tttagacgaa aaacgataca cgcaacccgg gcgcgtgcga
      841 cgaatggatc gaaaaagcgt aagcggagcc ggaacgctat taaattgcaa acatgcatag
      901 agcgcgactt cgtctatttc agacgaagca aaaccaatca ttaaccgcaa atcatcggaa
      961 atcaaccaac cgtttccgca gcgaacataa taaatttgtt ttactatcca cggcgcacaa
     1021 tagatcgtta tctcttcgct cgcgactgcc caatgcagat gatgtgaatt ttggtgaaag
     1081 tctccactcg gatgtgtatc tgaaaataaa atatcggacg gattctgaat cgttcccaag
     1141 ctggctttaa caaacgttat cttcgcatta tttttctttt ccagacgaca ggtcaaaccg
     1201 ataccggcag cataccgcgc gacatagttt tccaaatact ccggcggctt atcgccgaaa
     1261 aacacaataa agtaaacgga gttgttatta ttcatacgct tttttcggaa gatacaatat
     1321 gttcatacaa acgatgcacg tttttttcca tatcaaaata attatttaca aactgatatg
     1381 ccgatgccgt gcatgcacgc gtttgatcag attggattaa actctcagcc atggcatccg
     1441 ccagcgctaa cgcatttgca ggttcgcaga gtagtccggt ttccctgtgt ttcacgactt
     1501 ccggaatgcc cgatacatct gttgccacca tcggtgtttt aagaaacatg gcttcgatga
     1561 tcacattggg gataccatcc cgatcaccgt tggatgtaat aattgacggc ataacaaaaa
     1621 gatccgccga tgctatttct cgtagaacat gggtgttatt tagttctccg gccatggtca
     1681 cacaatcgga tacatgttcc tgttgaatgg ct
//
LOCUS       DAOQNQ010000479         1708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_52432_length_1708_cov_4.724743, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000479 DAOQNQ010000000
VERSION     DAOQNQ010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52432_length_1708_cov_4.724743"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            321..>1708
                     /locus_tag="PK754_16530"
     CDS             321..>1708
                     /locus_tag="PK754_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11162.1"
                     /translation="MAVAAQGFHQTHCFTGSWDVHSGMKTTKLSGPVIADNYDQGIRL
                     AMRLIEARENLADGSGMDITDWAQSVFGTERLVRLIETEPFSEKIYAPVIREEIERLA
                     KTWYPDVGKRHIVSINKLEDEFDVHVADDEAPSSPLATVSADQVLDFLSSRLTSAQLE
                     YLRRILKNSFGEDRDFSWNERIFRLLAESESYLQSNIDRMMAMYPDGPPLQENFNTGQ
                     TFSDEQIITCYKNVFLGINARFPIHFLATDGNRRAAILTQFAVEHILKTRPLDALQEY
                     TARDFSAIGLRNVARFFNYSLNRIIRNAYPDLVMPWEMAHVEDGFWTEEKNRTMAIRW
                     LIEEKLGIAKTDIPKALRDDRVTKSSFTQNGLSYMFVQYYKSVSRCIGAAYPEFMPWE
                     LGSVPNAYWQGEEGRQNVVKAIHWLMRRMQIIPAEIPERIKDKSINRELFSRFGLATV
                     FERIFKKNMFQV"
BASE COUNT          466 a          383 c          383 g          476 t
ORIGIN      
        1 ttattttttg gtgtttaggg ttaataaaac cacttgttca caaaatctgc gcaaatatat
       61 gatctcatgc cggcaaaagc aagctgtttt ttgctctaaa cttctttatt ttcagcatca
      121 tttccaataa tcacttatca ttttacgcat tcttacatgc ttacgcgaca ttgattaggt
      181 aggatactgc gaaaatcgta tatctccata cctgatcata tataagcctg ctttatctaa
      241 gagtttgagt tctgttggtt aaaaaaactc ccggcatcgt ttttcgaacc gggagtttaa
      301 aaaataatcg gagatagact atggccgtag ctgcgcaggg gtttcatcaa acacattgtt
      361 ttaccggttc gtgggatgta cattcaggca tgaaaacaac caaactttcc ggccctgtga
      421 tcgccgacaa ctatgatcaa ggtatccggc tcgccatgcg tctgatcgaa gcacgtgaaa
      481 atctagccga tggttcgggc atggatatta ccgattgggc gcagtctgtt tttggaacag
      541 agcgccttgt gcgactgatc gaaacagaac ctttttcgga aaaaatatac gcccctgtta
      601 ttcgcgaaga aatcgaacga ttagccaaga cgtggtatcc tgatgtaggc aaacgccata
      661 tcgtttctat caacaaactc gaagatgagt ttgacgtaca tgtcgccgat gatgaagcgc
      721 cttcatcacc gcttgcgacg gtatcggcgg atcaggtact ggattttctc agctcgcgtt
      781 tgacctcggc tcaactggaa tacctgcgcc gcattcttaa aaattctttc ggcgaagacc
      841 gcgatttttc gtggaatgaa cgtattttcc ggcttttggc cgaatcggaa tcctatctcc
      901 agtctaacat agatcgtatg atggcgatgt atcccgacgg accgccattg caggaaaatt
      961 ttaacaccgg gcaaactttt tcagacgaac aaatcatcac ttgctataaa aatgtttttt
     1021 tgggaatcaa cgcacgtttc ccgattcatt ttttagctac ggacggtaac cgaagagccg
     1081 ctattttgac acaatttgcc gtcgaacata ttctgaaaac ccgaccgctg gacgcactgc
     1141 aagaatatac ggcgcgcgat tttagtgcga tcggtttgcg caatgtggct cgatttttca
     1201 attattcgct gaatcgaatt attcgcaatg cctatcctga tctcgtcatg ccttgggaaa
     1261 tggcgcatgt cgaagacgga ttttggacgg aagaaaaaaa cagaacgatg gccattcggt
     1321 ggctgatcga agaaaaactc ggcattgcca aaacggatat tcctaaagct ctgcgggatg
     1381 atcgcgtaac caaatcatcc tttacacaaa acggattgtc gtacatgttt gtgcagtatt
     1441 acaaatcggt ttcacgttgt atcggtgcgg cataccctga gtttatgccg tgggaattag
     1501 gcagcgtacc gaatgcttac tggcaaggtg aagaagggcg gcagaatgtc gtgaaagcga
     1561 tacactggct catgcggcgc atgcaaatca taccggccga aatacccgaa cgtatcaaag
     1621 acaaatccat taatcgcgaa ctcttcagtc ggtttggcct cgccacggtt ttcgaacgca
     1681 ttttcaaaaa gaacatgttt caggtttt
//
LOCUS       DAOQNQ010000480         1704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_52689_length_1704_cov_3.361431, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000480 DAOQNQ010000000
VERSION     DAOQNQ010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52689_length_1704_cov_3.361431"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PK754_16535"
     CDS             complement(<1..658)
                     /locus_tag="PK754_16535"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11163.1"
                     /translation="MPDLLNRNRIARAILIKSPALFMRVVFGKKHLIAKIGIILALII
                     GPVLMFPDSDFTRYSEYKIGSISPDEVRAPFTFPVYKTPDQLTTERRDAQSLVAPVFD
                     RNSALAKSQLANLDEFINYLDLLRNAPKPFEYKDNDGQTQSYIPPAFDSVKSAIIRKF
                     GFSVMEERWAFLVNKENVTIDNNYGQMRSDTLRLLKRATIGRSLTRSQFLELSRDWMR
                     I"
     gene            complement(664..>1704)
                     /locus_tag="PK754_16540"
     CDS             complement(664..>1704)
                     /locus_tag="PK754_16540"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNB11164.1"
                     /translation="FPADNVRKMGEHGWMGIPFPKEYGGLGMSTVGYCLAVIELSKVC
                     ASHGITLGAHTGIGCGPIWLMGTNEQKLKYLAPLARGEKLGSFGLTEPGAGSDAGGTR
                     TFAENKGDHYVINGSKIFITSAGYADTFVITAVTDKTKGKSGISSFIVEKTFPGFIIG
                     KKEDKMGWRASDTRMLHFENMIVPKENLLGKPGEGFKGFLKVLDGGRVGVAALSLGIA
                     IGAYEEALQYSKTRMQFERPISDNQGIQFMLADMALGIQCSWHLMLHAARLKDAGQSF
                     TKEAAMSKLHATELAMQNCIKAIQILGAVGCTDRYNSERFFRDAKIGEIGEGTSEIQR
                     LIIAREILKTIA"
BASE COUNT          434 a          409 c          418 g          443 t
ORIGIN      
        1 gtatacgcat ccagtcgcga gacagttcca gaaattgcga ccgggtaagc gaacggccta
       61 tggtagcgcg tttgagcaaa cgaagggtgt cggaacgcat ttgcccgtaa ttgttatcaa
      121 tcgttacatt ttctttgttg accaaaaaag cccaacgttc ttccatcaca ctaaatccga
      181 attttcggat aatcgcgctt ttgacggaat caaatgcggg agggatataa ctttgtgtct
      241 ggccatcgtt atctttgtac tcaaaaggtt tcggcgcatt acgcaaaagg tccaagtagt
      301 ttatgaattc gtcgagattg gctaattgag acttagcgag cgccgaattg cgatcaaata
      361 ccggtgcgac taaactttga gcatcacggc gttccgtcgt cagttgatcc ggcgttttgt
      421 atacgggaaa cgtaaatggt gcgcgaactt catcgggtga aatcgagccg attttgtatt
      481 cggagtaacg ggtaaaatca ctgtccggaa acatcaacac agggccgatg atcagagcaa
      541 ggataatacc gattttagct ataagatgtt ttttaccgaa aaccacgcgc ataaagagag
      601 cgggggattt gatcagaata gcgcgcgcaa tgcgatttcg gttgagtaaa tcgggcatga
      661 gatttaagct atcgttttta atatttcacg cgcaatgatg aggcgttgta tttcagaagt
      721 accttcgccg atttcgccga ttttagcatc acggaaaaaa cgttccgaat tatagcggtc
      781 ggtgcatccg acggcgccga gaatctgaat ggctttgatg caattttgca tggccaattc
      841 cgtcgcatgc agtttggaca tagccgcttc tttggtaaaa gactggccgg catctttgag
      901 gcgagcggca tgaagcatca aatgccagct gcactggata cccagcgcca tgtctgccag
      961 cataaattgt atgccctgat tatccgaaat cggacgttca aactgcatac gtgttttaga
     1021 atattgcaac gcttcttcat acgcgccgat cgcgataccc agtgaaagcg cagcgacgcc
     1081 tacgcgaccg ccgtcaagaa ctttgagaaa acctttgaat ccttcgccgg gtttgccgag
     1141 cagattttct ttgggcacga tcatattttc aaagtgcaac atacgcgtgt ccgatgcgcg
     1201 ccaacccatt ttgtcttctt ttttcccgat gataaaaccg ggaaacgttt tttcaacaat
     1261 aaacgaactg atcccgcttt tacctttggt tttatccgta acggcggtga taacaaaagt
     1321 gtccgcgtaa cccgccgaag tgataaaaat tttagagccg ttgatcacat aatgatcgcc
     1381 tttattttcg gcaaacgtgc gtgtaccgcc cgcatcgctg cccgcgccgg gttccgttaa
     1441 tccgaaagaa ccgagctttt caccgcgtgc cagcggcgca agatatttca gcttttgttc
     1501 gttcgtaccc ataagccaga tcgggccgca accgatgccg gtgtgcgcgc cgagcgtgat
     1561 accgtgcgaa gcgcatactt ttgaaagttc gatcacagcc aggcagtagc cgaccgtgga
     1621 catgcccagt ccgccgtatt ctttaggaaa tggaatgccc atccatccgt gttcgcccat
     1681 tttgcgaaca ttgtcggcgg gaaa
//
LOCUS       DAOQNQ010000481         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_53819_length_1687_cov_6.114583, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000481 DAOQNQ010000000
VERSION     DAOQNQ010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53819_length_1687_cov_6.114583"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="PK754_16545"
     CDS             complement(<1..889)
                     /locus_tag="PK754_16545"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNB11165.1"
                     /translation="MKIAVLYGGNSSERNVSLNSGLAVISALHKMGHDVLTVDPAFGG
                     CIKEYEIKAVGELPPTEEELRGLPGSKNPHTIVEALNFISQKKVDVVFNALHGGIGEN
                     GALQAMMDVYGLRYTGSGSAASAIAMNKTLTKRIAETERVPSAAYVFLPRNTDQNQID
                     EIVRSKVSGKIVVKPNDDGSSVGLSIVDPGESLQEAFRKAAAIGDVLFESFIDGRELT
                     VAVLGDQTLPVVEIKPEGGVYDYKHKYTKGLTQYFCPAEIDPMISNKAQEFALKAFRS
                     VGCKGYARVDFRMNQSGELF"
     gene            complement(964..>1687)
                     /locus_tag="PK754_16550"
     CDS             complement(964..>1687)
                     /locus_tag="PK754_16550"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNB11166.1"
                     /translation="KVAAFAAFARVFIDTFNTLAIEWSEVLFWICVISMIGGNLMALV
                     QRDIKRMLAFSSISHAGYIIMAILAGPKLMDSNPNLAMDSILYYVMVYSLMNICAFTI
                     IHVFETREGRNLKVDDFAGFGFRYPLLSVAMSIAMLSMAGFPLTGGFIGKLRVFSAAV
                     DAGYVALTIVAVITSTISVYYYLGVMVKMYFTDAENPLEDIYLSKSTRTALALSSAGL
                     VLLGVIPTVIADVVRTVFQAFV"
BASE COUNT          490 a          414 c          327 g          456 t
ORIGIN      
        1 aaaaaagttc accggattga ttcattctaa aatctacccg tgcataacct ttgcacccga
       61 cggatcggaa tgcttttagt gcaaattctt gcgctttatt tgagatcatc ggatctattt
      121 ccgcagggca aaaatactgg gtaagacctt ttgtgtattt gtgtttataa tcatatacac
      181 caccctcggg ctttatctct acgactggca aggtctgatc accaagaact gcgaccgtca
      241 attctctacc gtcaataaaa ctttcaaata aaacatcgcc aatggccgca gctttccgaa
      301 aagcttcctg caatgattca ccgggatcta cgatactcag gcctaccgaa gatccgtcgt
      361 cattgggttt gaccactatt ttaccggaaa cttttgatcg tacaatttca tctatttgat
      421 tttgatcggt attccgcggc aaaaatacat atgcagccga aggcaccctt tccgtttccg
      481 caatacgctt ggtgagcgtt ttattcatgg ctatcgcgct tgccgctgaa ccggagccgg
      541 tatatctcaa accataaaca tccatcatgg cctgcaaggc gccattttct ccgataccgc
      601 catggagcgc attaaaaacg acatcaactt ttttttggct gataaaattt aatgcttcga
      661 caatcgtatg tggattttta cttcccggta aaccgcgcaa ttcctcttcg gtcggcggca
      721 attctcctac ggctttaatt tcgtactcct ttatacaccc gccaaatgcc ggatctaccg
      781 ttagtacgtc atgacccatt ttatgaagtg cggaaatcac cgcaagaccg gaattcaacg
      841 aaacattacg ttcgctggaa ttacctccat ataataccgc aattttcatc gttttgagtc
      901 gttaaaaaaa atagttctca tcgcttggat gagaactatt gaatggcatt tcatattttt
      961 tatttaaaca aaagcttgaa acactgtacg taccacgtca gcgatcaccg tcggtatcac
     1021 gccaagcaat acaagcccgg cgctgcttag tgcgagcgct gtgcgtgtgg acttggaaag
     1081 ataaatatct tcaagcgggt tttctgcatc cgtaaaatac attttgacca taacaccgag
     1141 ataataatat accgaaatcg tagaagtgat aacagcgacg atcgtcaagg ccacataacc
     1201 tgcatccaca gctgccgaga acactcttaa ctttcctata aatccgccgg taagagggaa
     1261 tccggccata gataacattg caatggacat cgcaacgctt aacaggggat agcgaaatcc
     1321 aaacccggca aaatcatcta ccttgaggtt tcgaccttca cgtgtttcaa aaacgtgaat
     1381 gattgtaaac gcacatatat tcatcagcga ataaaccatg acgtagtaga gtatcgaatc
     1441 catcgccagg ttaggattgc tatccattaa cttcggtccg gcgagtatag ccatgatgat
     1501 atatccggca tgcgaaatac tcgaaaatgc aagcatacgc ttgatatcgc gttgtaccaa
     1561 ggccatcagg tttccgccga tcatactgat cacacagatc cagaaaagca cttcagacca
     1621 ttcgatcgct aacgtattga aagtatcaat gaatacacgc gcaaaggcag cgaaagccgc
     1681 tactttt
//
LOCUS       DAOQNQ010000482         1659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_55797_length_1659_cov_4.443890, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000482 DAOQNQ010000000
VERSION     DAOQNQ010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55797_length_1659_cov_4.443890"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PK754_16555"
     CDS             complement(<1..341)
                     /locus_tag="PK754_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017716009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNB11167.1"
                     /translation="MATEEKKSTYRFETLQLHAGQKPDSATNARAVPIYQSTSYVFNN
                     ADHAAALFGLQEFGNIYTRIMNPTTAVFEERIAALEGGVAAVATSSGQAAQFLAITTI
                     AQAGDNIVATS"
     gene            complement(374..1579)
                     /locus_tag="PK754_16560"
     CDS             complement(374..1579)
                     /locus_tag="PK754_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HNB11168.1"
                     /translation="MKFGTSAIHAGVNKDTAYNSVTTPIYTSSTFRFEEPGLTKGYDY
                     TRSGNPTRSALEENIAALEGGICARAVATGMAAVATALHFFKSGDHILCTHDCYGGTE
                     RLLRTYSELFGLRVTYVDMCDLKSVEAAWLPETRGIWIETPSNPLLNIVDIRAITAIA
                     RARRGLSIVDNTFLSPYFQRPFELGADIIVHSTTKYLNGHSDVVGGAVVVNDSNLADR
                     LKFLVNALGLGAAPFDCWLVLRGIKTLVPRMQAHERNALAVARFLSEHPQVRRVFYPG
                     LPSHPNHALARCQQYGFGGMVSFELDGDSESMRRLLKGTKLFTLAESLGGVESLIEHP
                     ATMSHASMDAELREEAGINDRIVRLSVGIEETSDLIEDLASALRQCQYNTSATPASAR
                     SVFSSINAH"
BASE COUNT          422 a          437 c          394 g          406 t
ORIGIN      
        1 aaactggtag cgacgatatt atcaccggcc tgagcgatcg tcgtgatagc cagaaattgc
       61 gccgcttgac cggacgatgt agccaccgcc gcgacaccgc cttctaaggc ggctatgcgc
      121 tcttcaaaaa cagccgtcgt aggattcata atgcgcgtgt atatattgcc gaattcctgt
      181 agcccaaaaa gcgctgccgc atgatcggca ttattaaata catacgacgt actttgataa
      241 atcggaacgg cgcgcgcatt ggtagcgcta tccggtttct gacccgcatg aagttgcaat
      301 gtttcaaaac ggtaggttga ttttttttct tccgtagcca tgatggacct ttctttacgt
      361 gtttatgtat gaattaatga gcattgattg atgaaaaaac agatcgcgcc gacgcgggcg
      421 ttgcagacgt attgtattga cactgacgca aagccgacgc caagtcttca atcaaatcgg
      481 atgtttcttc gataccgacc gataagcgta caatccggtc attgataccg gcttcttccc
      541 gcaactcggc atccatcgaa gcatgagaca ttgtggccgg atgttcgatc agggattcta
      601 cgccgccgag ggactccgcc aacgtaaaca atttggtacc tttgagaagc cggcgcatag
      661 actccgagtc cccgtccagt tcaaatgaaa ccattccgcc aaaaccgtat tgctgacatc
      721 gagccaaggc atgatttgga tgtgacggaa gtccggggta aaaaacccgc cgtacctggg
      781 gatgttcgga gagaaatcgc gctaccgcca gcgcattgcg ctcatgcgct tgcatgcgcg
      841 gcacaagggt tttgatcccg cgcagtacaa gccagcaatc aaaaggcgcc gcacccaaac
      901 ccaatgcgtt aacaagaaat ttgagacggt cggccaaatt tgagtcattg acaaccacgg
      961 caccgccgac tacatcgctg tgaccgttga ggtatttagt cgtcgaatgc acgatgatat
     1021 cggctcccaa ttcaaatggc cgctggaaat acggcgagag aaatgtgtta tctacaatgg
     1081 ataaacctct tcgtgcacgc gctatagccg taatcgctcg gatgtccacg atattgagca
     1141 acggattgct tggcgtttcg atccatattc cgcgcgtttc aggcaaccat gcagcttcta
     1201 ccgatttcag atcacacatg tctacatacg taacacgcag accaaacaat tcactgtacg
     1261 tcctcagtaa tctttctgtg ccgccgtaac aatcatgtgt acacaaaata tgatcgccgc
     1321 ttttgaaaaa atgcagcgcc gtcgcgacgg ccgccattcc tgtagctacg gcacgcgcgc
     1381 atatcccgcc ttcaagcgcg gctatatttt cttccaaagc cgagcgcgtc ggattgcccg
     1441 aacgcgtata atcatatcct ttagtaagtc ccggctcttc aaatcgaaat gtcgaagacg
     1501 tatagatcgg tgtggtgacg ctgttgtagg ccgtatcttt gttgaccccg gcatggatcg
     1561 cagaagttcc gaatttcatg atgttccttt cattttaaaa aaaataaacc tctcccggga
     1621 gattggaaga ggcctaggct tcttctctca tctttcccg
//
LOCUS       DAOQNQ010000483         1639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_57215_length_1639_cov_5.460859, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000483 DAOQNQ010000000
VERSION     DAOQNQ010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57215_length_1639_cov_5.460859"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..421
                     /locus_tag="PK754_16565"
     CDS             131..421
                     /locus_tag="PK754_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNB11169.1"
                     /translation="MAEEKKMQEIQIHFPPEKQSGTYANNMVVAHSKEEFILDFMLVT
                     PPTGVVNARIVMSPGHVKRVIAALAQNMRHYEEQFGKIEEASEPKNPFGFQA"
     gene            424..1281
                     /locus_tag="PK754_16570"
     CDS             424..1281
                     /locus_tag="PK754_16570"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNB11170.1"
                     /translation="MSRIKIIDIHIHIQPWWQLKPEIRTRMAHGKENYDQLLDIMKNP
                     AKLIALMDKADIEKVGLINYVSPDLMGFDDSSNDFSSAYQKYAPDRFIAYGSVHPRFT
                     KDAEADIHRLIHDLGIRAIKVHPPHQLYYPNDYLNGMKNLEIIYRRCEEWGIPVMIHT
                     GTSFFAGARIKYGDPIHVDDVAVDFPNLKIIMAHGGRPLWMETAFYLVRRHKNVSIDI
                     SSIPPKKILTDYFPRLEEIADKVYFGTDWPGPQVDDMRKNADEFLSLPLDETAKLKIV
                     RENALKLFG"
     gene            1341..>1639
                     /locus_tag="PK754_16575"
     CDS             1341..>1639
                     /locus_tag="PK754_16575"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNB11171.1"
                     /translation="MIFIRCIFSSLWLLFVAVVPIFSQEKPVAAILSFHNTSEKFYLD
                     EVAKSFPTLLKTELAQTRSVVIVEREKLDAVLKEQDFVLSDLSEDKDKQNPVLSIP"
BASE COUNT          482 a          331 c          358 g          468 t
ORIGIN      
        1 caaagcgtgg ttatcaaaaa atggagctta tcaaatcaat gatcgtaatt tgtcagaact
       61 aaagcgattg tgtgaaaagt aaatttttat tatttaactg atagcgttaa gaataacctc
      121 tgaggagttt atggctgaag aaaagaaaat gcaggaaatc caaattcact ttcctcctga
      181 aaaacaatcc ggaacgtatg ccaataatat ggtcgtagcg cactccaaag aagaatttat
      241 actcgatttt atgctggtaa cgcctccgac aggtgtcgtt aatgctcgaa tcgttatgag
      301 ccccggtcat gtcaaacgtg tcatcgcggc ccttgcacaa aatatgcgcc actacgaaga
      361 gcaattcggt aaaatcgaag aagccagcga acctaaaaat ccatttggtt ttcaggcgta
      421 atcatgtctc gtatcaagat tatagatatt cacattcata tccagccgtg gtggcaattg
      481 aagccggaga tacgcacgcg tatggcccac gggaaagaga actacgatca acttctggat
      541 attatgaaaa atccggccaa gttgatcgcg ttgatggata aggccgatat agaaaaagta
      601 ggtttgatca actacgtaag ccctgatctg atgggttttg acgattcatc caatgatttt
      661 tcgtcggctt atcaaaaata tgctccggat cggtttatcg cctatggatc tgtacatcct
      721 cgattcacca aagatgccga agcggatata caccgattga ttcacgatct cggcattcgc
      781 gccatcaagg tgcatccgcc gcatcagctc tattatccaa atgattatct gaacggcatg
      841 aaaaacttgg aaattattta ccgtcgctgt gaagagtggg gaataccggt gatgatacat
      901 accggtacgt ctttttttgc gggagcgcgt atcaagtacg gtgatccgat tcatgtggat
      961 gatgtagcgg tagattttcc caatctcaaa atcatcatgg cgcacggcgg aaggccgcta
     1021 tggatggaga cggcttttta tcttgtgcgc cgtcataaaa atgtgtcgat tgatatttcc
     1081 tccattcctc ccaaaaaaat actgacggat tattttcccc gtcttgagga aatagcggat
     1141 aaggtatatt ttggtacgga ttggccgggc ccgcaggtag atgatatgcg taaaaacgcc
     1201 gatgaatttc tttctttacc gctcgatgaa accgccaaac taaaaatagt tcgtgaaaat
     1261 gctttgaaac tttttggttg aaattcgttt acctatacgt cgtttcttga atgaacgata
     1321 ttcagaatat aggggtactc atgatcttta tacgttgtat ttttagtagt ttatggttgc
     1381 tgtttgttgc cgtggttccg attttttcac aggaaaaacc ggtggctgct atcctaagct
     1441 tccacaacac ttcagagaaa ttttatttag acgaagtagc aaaatccttt cccaccttac
     1501 tcaaaacgga actggcacaa acgcgctccg ttgtcatcgt cgaacgtgaa aagctggatg
     1561 cggtattgaa agaacaggat tttgttttaa gtgatctgtc cgaagataag gataaacaaa
     1621 atcctgttct ttcaatacc
//
LOCUS       DAOQNQ010000484         1633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_57657_length_1633_cov_4.924588, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000484 DAOQNQ010000000
VERSION     DAOQNQ010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57657_length_1633_cov_4.924588"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PK754_16580"
     CDS             complement(<1..762)
                     /locus_tag="PK754_16580"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNB11172.1"
                     /translation="MLRWLLRSYAVFCINLFYVAAQDSWPSPYFDPVSYDYGYKDSLG
                     RLVLPVQYDDARMFVHGIAAVKQNGLWFYIRKDGSTVFNGKYEVAGSFSEGYAVVWDK
                     AEGHRLIDTTGRVQLRQANMKDVIRGRLVTSNPRQLPLTGLMGLQTLDGKTIVEPFFP
                     DDESYVIRNDNGLFSTEGMQPPDPQRIPFQLRGLWGFVDAQGRIVMMPRYREARPFQE
                     GLAAVRVDSLWGYIDTAGRFAIAPQFDDAKMFSEGL"
     gene            complement(875..1336)
                     /gene="ruvX"
                     /locus_tag="PK754_16585"
     CDS             complement(875..1336)
                     /gene="ruvX"
                     /locus_tag="PK754_16585"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNB11173.1"
                     /translation="MEKTMAFFMEKGRILAIDYGERRVGLAITDPMQIIASGLTTLAV
                     ENDRHAANQIVQIISKEDVTEIIIGLPIAPSGEATEKTRTVNQFISILKEKTALPIIP
                     WEERFSTVSAQRLLIEAEEKKKRRTKEKIDTLAALVILQHYLDYLRLNPKK"
     gene            complement(1367..>1633)
                     /locus_tag="PK754_16590"
     CDS             complement(1367..>1633)
                     /locus_tag="PK754_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNB11174.1"
                     /translation="GQKIHEVEHVVDIVFDSIREILSEADPDVRIEVRDFGVFEVKTT
                     KAKPKARNPKTGEIIFVPPRRKTHFKPGKLLKEVLKQPLKGEQE"
BASE COUNT          451 a          406 c          331 g          445 t
ORIGIN      
        1 taatccctcg ctaaacattt tggcatcatc aaattgcgga gctatcgcaa aacgtcccgc
       61 cgtatcgata taaccccata acgaatccac acgcaccgca gctaaacctt cttgaaacgg
      121 gcgcgcctcg cgataacgcg gcatcataac gatccgccct tgtgcatcta caaaacccca
      181 taatccacgc agttgaaaag gaatccgctg cggatcggga ggttgcatac cctccgttga
      241 aaaaagacca ttatcatttc gaatcacata cgactcatcg tctggaaaaa aaggttccac
      301 gatagtttta ccgtctaacg tctggagccc catgaggcct gtcaacggca attggcgcgg
      361 attacttgtg accaaacggc ctcggataac atctttcata ttggcctgcc ggagctgaac
      421 acgccccgtc gtatcaatga ggcgatggcc ttcagcttta tcccaaacga ccgcgtaacc
      481 ttcgctgaag cttccggcta cttcgtattt accgttaaac accgtggagc cgtctttacg
      541 aatataaaac cacaagccat tttgtttgac tgctgcgata ccgtgcacaa acatacgcgc
      601 atcatcgtat tgcaccggca aaaccagacg ccctaaacta tccttgtatc catagtcgta
      661 cgataccgga tcaaaataag gcgacggcca gctatcctgt gccgctacgt aaaaaagatt
      721 aatacaaaaa acggcatagc ttcgtaaaag ccatcgtaac ataaatatct gtggttaaat
      781 aaaaaaagat tgccgaaagg cattagaaca gtatctaaaa ctaacacgaa cgcttaaaaa
      841 aaccaaaaaa aaacgtaccg gctggcgctg atgtttattt tttcggatta aggcgcaggt
      901 aatcgagata atgctgaaga atcaccaacg ccgctaacgt atctattttt tctttggtgc
      961 ggcgtttttt cttttcttcc gcctcgatca aaagtcgttg cgctgacacg gtggaaaaac
     1021 gttcttccca gggaataata ggaagcgctg ttttttcttt aaggatggat atgaattgat
     1081 tgaccgtacg cgttttttcc gtagcctcgc cgcttggcgc tatgggcaga ccaattatta
     1141 tttccgtaac atcttctttt gaaattattt gaacaatctg attggcagca tggcggtcat
     1201 tttcgaccgc cagcgtcgta aggccggatg caatgatctg catcggatct gtgatcgcaa
     1261 gaccgacacg ccgttcaccg taatcaatag ctaaaatgcg tcctttttcc ataaaaaaag
     1321 ccatggtttt ttccatggct tattattcat ataaaattat ttctttttat tcctgctcgc
     1381 ccttcagggg ttgtttcaaa acctctttga gcagcttacc gggtttaaaa tgtgttttac
     1441 gacgcggagg aacaaatata atctctccgg ttttcggatt gcgtgctttg ggtttggctt
     1501 tggttgtttt aacttcaaac actccaaaat cgcgcacttc gatcctgaca tccggatcgg
     1561 cctcggagag tatctcccgg atcgaatcaa atacaatatc tacaacgtgt tccacttcgt
     1621 gaattttttg acc
//
LOCUS       DAOQNQ010000485         1628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_58041_length_1628_cov_4.560076, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000485 DAOQNQ010000000
VERSION     DAOQNQ010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58041_length_1628_cov_4.560076"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PK754_16595"
     CDS             complement(<1..435)
                     /locus_tag="PK754_16595"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11175.1"
                     /translation="MPIPWFRQFEKADTPKSTLHGESQPFVSYYDDPETEYRRAREQA
                     ILIDTSFYGTLQLQGRHTLSLLHRLTTQDIQNMTSNTVQQTVLTSDRGRIMDLLRVFH
                     GDQSTTLRTSAERSATIAQWLDKYIIMDDVRINDISDSISSVF"
     gene            590..>1628
                     /locus_tag="PK754_16600"
     CDS             590..>1628
                     /locus_tag="PK754_16600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11176.1"
                     /translation="MAKLQSKERQKKSETSTATGLQIKPWQQTAISILILYALNVFLF
                     SDIVIKGKAFSSGADNSAATAISRGMGELVKQEGDLPLWNPFMFAGMPSFASGMYGNP
                     DATPLIKYQKYFNPSYYVSLVVNLFFGNRDNVWEVATFFLAGVFMFLLARSLGFGHLI
                     ALTAAVSYMFCNFFVASMAAGHGGKVRTIAYIPLVVWSVFNFFQNRSVWTWAIMGLVL
                     GLFFQEAAHTQIIYYAFLMLGIYCVVYLVDEFRNNLSGVIRNGLGFTAALITGLGFGA
                     LGQIALYVYSDVTMRSMGPAFAETTEMAGAGGMTFDYITMWSFHPLESVTFFIPGFFG
                     LESPYYWGWMTF"
BASE COUNT          376 a          341 c          404 g          507 t
ORIGIN      
        1 aaatacagag gaaatactat cggaaatatc atttatccga acatcgtcca tgatgatgta
       61 tttatcaagc cattgggcga tcgttgctga ccgctccgcc gatgtgcgaa gtgtcgtact
      121 ctggtcgccg tggaatacac gcaataaatc catgatgcga ccacgatcgg aagtgagtac
      181 cgtttgctgt acggtatttg aagtcatgtt ttgaatatct tgcgtggtca aacgatgtag
      241 taaactcagc gtgtgacggc cttggagttg gagtgttccg taaaaagaag tgtctatcag
      301 gatcgcttgc tcacgcgccc gtcggtattc ggtttccgga tcatcataat acgatacgaa
      361 tggttgcgat tctccgtgga gcgtgctttt cggagtgtcg gctttttcaa attgtctgaa
      421 ccatggtata ggcattatcg tcgctttcta ttcatatttt gcgcggcaat atacttcatc
      481 catacgaaca gcgcaagcat gtccacggtt tgaggtaaat tctcttttga tattccttcg
      541 tttttcgtaa actttgccgc attactatca ataaccattg aatcggatta tggcaaaatt
      601 acaatcaaaa gaacggcaaa aaaaatcgga aacctccacc gcgacgggac ttcaaatcaa
      661 accctggcaa caaaccgcga tcagtattct tattctctat gcactcaatg tgtttctttt
      721 tagtgatatt gtgatcaaag ggaaagcctt ttcgagcggt gcggataatt cagcggctac
      781 agcgatctcc cgcggtatgg gcgaattggt caagcaagaa ggtgatcttc cgctatggaa
      841 cccctttatg tttgcaggca tgcccagctt tgccagcggt atgtacggca accctgacgc
      901 taccccgctg atcaagtatc agaagtattt taacccttcg tattatgtaa gcctggtcgt
      961 taatttgttt ttcggtaacc gggataatgt gtgggaagtg gcgacttttt ttcttgccgg
     1021 tgtatttatg tttttactgg cacgcagtct tggatttggc catttaatag cactaacggc
     1081 ggccgttagt tatatgtttt gcaatttttt tgtcgcctcg atggcggccg gccacggcgg
     1141 aaaagttcgt acgatcgctt acattccgct ggtcgtgtgg agtgttttta attttttcca
     1201 aaaccgcagc gtatggacgt gggcgatcat gggacttgtt ctagggttgt tttttcagga
     1261 agcggcgcac acgcaaatca tttattatgc ttttctgatg ctgggtattt attgcgtcgt
     1321 ttatcttgtt gacgaatttc gtaacaatct gtcgggcgtg attcgaaacg gtttgggttt
     1381 caccgccgcg ctgataaccg ggttgggatt tggcgcactt ggccaaattg cgctgtatgt
     1441 gtattccgat gttacgatgc gaagtatggg gccggcattt gccgaaacaa cggaaatggc
     1501 cggtgccggt ggtatgacat ttgactatat cacgatgtgg tcatttcatc cgctggaatc
     1561 tgttacattt tttataccgg gcttctttgg acttgaaagc ccgtattatt ggggttggat
     1621 gacgttta
//
LOCUS       DAOQNQ010000486         1620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_58659_length_1620_cov_4.021725, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000486 DAOQNQ010000000
VERSION     DAOQNQ010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58659_length_1620_cov_4.021725"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PK754_16605"
     CDS             <1..421
                     /locus_tag="PK754_16605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB11177.1"
                     /translation="DELGLISALDTLCRTQFGAQGIHVDFQTFDVDQRLPQDIELPIY
                     RVVQEAFQNITKHAKATQVTLQLIGYDDHVVVTIEDNGIGFDLASINEIKKAPFGMGL
                     PGMRERIEFLSGKIDIDSSPGNGTSVVIEVPFKKNEV"
     gene            431..1102
                     /locus_tag="PK754_16610"
     CDS             431..1102
                     /locus_tag="PK754_16610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB11178.1"
                     /translation="MEKTDVLIADDHPMVREGLRSLLETEKGFNVVAEAGDGEEALKY
                     MERHKPMMAILDISMPKLNGIDTARQILKRWPKTRVVMLTMHHDEAFLVKVMEIGVQG
                     YLLKDSAGDEILRALKRIAEGEKYFSQSAMEAIGRHISKKNDLNVLADKEAAERLTKR
                     ELQILSYIAEGMSTPEIAKKLYLSPRTVDTHRANIMHKLNIHQATGLVRFALENGLFA
                     RDKTK"
     gene            1224..>1620
                     /locus_tag="PK754_16615"
     CDS             1224..>1620
                     /locus_tag="PK754_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11179.1"
                     /translation="MDRNYKNLIFVILIFSLFQTKTGFANVTVNSASVTPTGSNQNGQ
                     YTIAFATGNGTTNLIAGTSQIIITFNASTYVPASISASLVTVNSTQATNVTVSGQVVT
                     ITTPVAIAKNGGTGTVVISSSAGILNPATA"
BASE COUNT          487 a          305 c          376 g          452 t
ORIGIN      
        1 ggacgaactg ggcttgattt ctgcgttaga tacattgtgt cgtactcagt ttggcgcaca
       61 gggtattcat gtcgattttc aaacattcga tgtagatcag cgcctgccgc aggacataga
      121 actgccgata taccgtgtcg tacaagaggc ttttcaaaat attaccaaac atgctaaagc
      181 cacacaggtc accctccaat tgatcggata cgatgatcat gtcgttgtta cgatcgagga
      241 taacggaatt gggttcgacc ttgcttcaat taatgaaata aaaaaagcgc cgtttggcat
      301 gggtttaccg ggaatgcgcg agcgtattga atttttgagc ggaaaaatag atattgattc
      361 gtcaccgggt aacggcacat ccgttgttat cgaggtgcca tttaaaaaaa atgaagtgta
      421 gtagtgtcat atggaaaaaa cggacgtatt gatagctgat gatcatccga tggtacgtga
      481 agggttgcgt tcattgcttg aaactgaaaa aggttttaat gtcgtggccg aagcaggaga
      541 cggggaagag gcgctgaagt atatggaacg ccataaaccg atgatggcca ttttggatat
      601 ttcaatgccc aaattaaatg gtattgatac agctcgtcag atattaaaac gatggccgaa
      661 aacccgcgta gtaatgctga ccatgcatca tgacgaagcg tttcttgtaa aggtcatgga
      721 aataggtgtt caagggtatt tgctcaaaga ttctgccggc gatgaaatac tgcgggctct
      781 caagcggatc gcggaaggtg aaaagtattt tagtcaaagt gctatggaag cgataggccg
      841 tcatatttca aaaaagaatg atcttaatgt tttggctgat aaagaagctg cagagcgctt
      901 aacaaagcgt gagttgcaaa ttttgagtta tattgcggag ggtatgagta cgccggagat
      961 tgctaaaaaa ttatacctca gcccgcgaac tgtggataca catcgtgcca atataatgca
     1021 taaattaaat attcaccaag ctacaggatt agttcggttt gcattagaaa acggactttt
     1081 tgccagagat aaaactaagt aaaaacacct aagttaaaac tctacataaa ttacttccct
     1141 ctacgtattg ttaaagccac ccattctata gtatatagcg attgatttat caagagttgt
     1201 aaactatttg gatggagtgt aagttggacc gcaattataa aaatctgatt tttgtcattt
     1261 taattttttc gctgtttcag acgaaaaccg gttttgcaaa tgttacagta aattctgcaa
     1321 gtgttacccc tacgggttcc aatcaaaatg gacaatacac gattgccttt gcaactggaa
     1381 atggcacgac aaatttgatc gcgggtacaa gtcagatcat cataactttt aatgcatcaa
     1441 cctatgtgcc ggcctccata tcggcctctt tagttacggt caatagtacg caagcgacga
     1501 atgtcaccgt ttccggacaa gtcgttacga ttacgacgcc cgtagccatt gcaaaaaatg
     1561 gcggtacggg tacggttgtt atttcgtcct ctgctggtat tcttaatcct gctacggcgg
//
LOCUS       DAOQNQ010000487         1587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_61238_length_1587_cov_7.793081, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000487 DAOQNQ010000000
VERSION     DAOQNQ010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61238_length_1587_cov_7.793081"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PK754_16620"
     CDS             complement(<1..744)
                     /locus_tag="PK754_16620"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNB11180.1"
                     /translation="MMKDYFLITLFFFVISFGRSQDLAENTKPRIRKSETLLVYPPAN
                     HAPIHKATAVHLFAFMALIGRSDITPSDPRGLAVTRLKSTDDPDNRNDDDDLTAYGVN
                     SGQNNIIFNSSMQGLDVYEGKGPQQKLKRPRGIACNSDGDVYIADTGNNRLVKLKNPG
                     GYLLFVKASGKKGNKSGEYDEPRGVAVDQKNQVYVTDYKNDRIQVLDSNLIPCQIWGN
                     ATDASGTLSLYRPDAIAASDGSDPDNFTQD"
     gene            complement(741..>1587)
                     /locus_tag="PK754_16625"
     CDS             complement(741..>1587)
                     /locus_tag="PK754_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNB11181.1"
                     /translation="LLNRLYQTLPTSENKRQVLDWLSEYFHRFDASDDIADRLFDFIR
                     QFRLTTNAERNKEITAAFPESDLKYFVNKTSERIAANAPKTPYKSQKDERIDFCLRYD
                     IAVPGIIPTMDEAKIILSGKDNWDEQLQTLLMIEKMGMKPKPLESQLIAIFSKRNLEH
                     KETLTEMQSSAIITLGHLKTSAPAAIDFMMASLMSYDYRFSDNAKTALIQIGTSAVPK
                     LISKLQSLTDQDGGLMYIIAEILGKIGPDARSAKTILQQSLGKTNNSDVRYAIESALQ
                     AIEGQ"
BASE COUNT          403 a          328 c          379 g          477 t
ORIGIN      
        1 gtcttgtgtg aaattatccg ggtcactgcc gtctgacgcg gcaatggcat cgggacgata
       61 cagtgacaat gtaccggaag cgtcggtcgc attgccccaa atctggcacg ggatgaggtt
      121 ggaatctaat acctgaatgc gatcattttt atagtcggtc acatagactt gatttttttg
      181 atctacggct acgccgcgcg gttcatcata ttcgccggat ttatttcctt tttttcctga
      241 agctttgacg aaaagcaagt atcctccggg attctttagt ttgacgagac gattattacc
      301 ggtatcggct atataaacat caccatcact attgcaagcg atgccacgcg gacgtttgag
      361 tttttgctga gggcctttac cttcatacac gtctaagccc tgcatagagc tgttgaagat
      421 gatattattc tgaccggaat tgacaccata agccgtgaga tcatcgtcgt cattgcgatt
      481 atcgggatcg tcggtagatt tcaatcgggt aacggccaaa ccgcggggat ccgaaggcgt
      541 gatgtcggag cgtcctatca atgccataaa tgcaaataaa tgaactgccg tggctttgtg
      601 aatcggtgcg tgattagcgg gcggatatac cagaagagtt tctgattttc gaatgcgagg
      661 ttttgtgttt tccgcaagat cttgagatcg gccaaacgaa ataacaaaga aaaacagggt
      721 tattaaaaag taatctttca tcattgcccc tcgatggctt gtaacgccga ttcgatggca
      781 taccgtacat cggaattatt agtcttgccg aggctttgtt gcaaaatggt ttttgcagaa
      841 cgtgcatccg gaccgatctt accaagtatt tcagcgatga tgtacataag tccgccgtcc
      901 tgatcggtta atgattgcaa tttggatatg agcttgggta cggccgatgt gcctatttgt
      961 ataagcgctg ttttagcatt atccgaaaac cggtaatcgt aactcataag cgaagccatc
     1021 ataaaatcaa ttgccgcagg ggccgaagtt ttcaaatgcc ctaaggtgat tattgccgat
     1081 gattgcattt ctgtcagtgt ttctttgtgc tccaaatttc gcttggagaa tatcgcgata
     1141 agttgcgact ctaaaggctt aggcttcatt cccatttttt caatcatcaa caatgtttgc
     1201 aactgctcat cccaattatc tttaccgcta agtattattt tggcttcgtc catggtaggt
     1261 atgatgccgg gtacggcaat gtcataccgc aaacagaaat caatgcgttc gtctttttga
     1321 cttttgtaag gagtttttgg tgcattggcg gcaatgcgtt cggacgtttt atttacaaag
     1381 tatttcaaat ccgattcggg aaatgcagcg gttatttctt tgtttcgctc tgcattcgtg
     1441 gtcaggcgaa attgacggat gaaatcaaaa aggcgatcgg ctatatcgtc cgaggcatcg
     1501 aaacgatgga aatattcgct gagccaatcc aatacttggc gtttattttc agatgtaggc
     1561 aaagtttgat acagacggtt gagcagg
//
LOCUS       DAOQNQ010000488         1585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_61386_length_1585_cov_4.739216, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000488 DAOQNQ010000000
VERSION     DAOQNQ010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61386_length_1585_cov_4.739216"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PK754_16630"
     CDS             complement(<1..411)
                     /locus_tag="PK754_16630"
                     /inference="COORDINATES: protein motif:HMM:NF017346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HNB11182.1"
                     /translation="MPSTKRNKSETSESLAIKGLGVSDGFAIGPVYVLHADNFRIHER
                     TLKSEDEIAQETSRFQQALDVTREQLDQLIRDAKSKYGKEYSAILESHFLMLEDQEMI
                     RGTLKNIKSVKMNAEAAFAAVIDKFKTGFLKSNNE"
     gene            complement(412..678)
                     /locus_tag="PK754_16635"
     CDS             complement(412..678)
                     /locus_tag="PK754_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNB11183.1"
                     /translation="MIEREITVVNKAGVHARPSAMIVKTASQFDSEVFLSCDGTEVNA
                     KSIMSVMMLAAAQGSVVKIKTEGSDEMQAADAIAALFASKFNEE"
     gene            complement(714..>1585)
                     /locus_tag="PK754_16640"
     CDS             complement(714..>1585)
                     /locus_tag="PK754_16640"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNB11184.1"
                     /translation="LFQSGGKRMRAVMTVLSCALFEKNYKKSFPAAAAVELLHNFSLV
                     HDDIMDNDDLRRGRETIHKKWNANVAILTGDLLHALAFKSLNGSPESVLPRVLDIFSD
                     AYIGLVEGQALDKEFEKRSDIKLPDYLKMIELKTAGLFAAAAQIGAVVGKADSKKEQA
                     LRDYGYYCGMAFQIQDDLLDVIGDEATLGKDICSDLMEGKKSYIALHCVQDKYGKDFL
                     SVFQNVTDPDILKRVIEDFRKFIQKTGIQEKTEKAIAKYVQSAVQSLDRIKDSSAKAQ
                     LVAIAHRAGQRKN"
BASE COUNT          384 a          381 c          354 g          466 t
ORIGIN      
        1 ctcattattg gacttaagaa aaccggtttt aaatttatct atcacggccg caaatgccgc
       61 ttcggcattc attttgacgg acttgatatt tttcagcgtg ccgcggatca tctcctgatc
      121 ctcgagcatc aaaaaatgtg attcaaggat cgcgctgtat tctttaccgt atttggattt
      181 agcgtcccgg ataagctgat ccagctgctc tcgtgtgaca tcgagcgctt gttggaaccg
      241 tgatgtctcc tgtgcaattt cgtcttcgct tttgagcgtg cgttcgtgga tacgaaaatt
      301 atcggcgtga agtacgtaga ccggcccgat cgcaaatccg tcggaaacac ccagcccttt
      361 gatggctagg gactccgatg tttcggattt atttcgtttg gtcgaaggca tttattcttc
      421 gttaaatttg gaagcaaaca aagccgcgat cgcatcggcc gcttgcattt cgtcggaacc
      481 ctcggttttt atttttacga cgctgccttg agccgccgcg agcatcatga cgctcatgat
      541 acttttggca ttgacttcgg tgccgtcgca ggataaaaac acttcggaat caaactggct
      601 tgcggtttta acgatcatcg ccgacgggcg tgcatggacg ccggctttat tgacaacagt
      661 gatttccctt tcgatcatgg attcggcggt ggtcctcggt gtgaaacgat ggttcagttt
      721 ttacgctgcc cggcgcggtg tgcgatagct accaattggg ctttggcgct gctatctttg
      781 atgcggtcca gactttgaac ggccgattgg acatacttgg caatcgcctt ttccgttttt
      841 tcctgaatac ccgttttttg gatgaatttt ctaaaatctt cgataacacg cttgagaata
      901 tcggggtcag tgacattctg aaaaacggaa agaaaatctt tgccgtattt atcctgtaca
      961 caatgaagcg cgatgtacga tttttttccc tccataagat cgctgcatat atctttaccc
     1021 agcgtcgctt catccccgat cacatccagt aaatcgtcct gaatctgaaa cgccataccg
     1081 caataataac cgtaatcacg taaagcttgc tcttttttgg agtccgcttt tccgactaca
     1141 gcaccgatct gggcggcggc ggcaaataaa ccggcggttt tcagttcgat cattttaaga
     1201 taatcaggga gttttatatc cgaacgtttt tcaaactctt tatccaatgc ttggccttcg
     1261 accaatccga tataggcatc cgaaaaaata tccaatacac gaggtagtac ggattcagga
     1321 gaaccattta atgatttgaa agccaaagca tgcaataagt caccggtgag aatagcgacg
     1381 ttcgcattcc attttttatg tatggtctcc cgtccccgcc gcaagtcatc attgtccata
     1441 atatcgtcat ggaccagtga aaaattatgc aaaagctcca cagccgctgc ggcagggaat
     1501 gattttttat aatttttttc aaacaatgcg caggacaata cggtcatcac tgcacgcatt
     1561 cgcttgccgc cgctttgaaa aagat
//
LOCUS       DAOQNQ010000489         1578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_61961_length_1578_cov_5.211425, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000489 DAOQNQ010000000
VERSION     DAOQNQ010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61961_length_1578_cov_5.211425"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PK754_16645"
     CDS             complement(<1..350)
                     /locus_tag="PK754_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB11185.1"
                     /translation="MIRRFFIGTVLFWLSALHAQQNITFETLSIEQGLSQTVVYSIVQ
                     DSRGFLWFGTQDGLNRYDGYNFTVYKNNHLDTASLSDNRILKVFKDRSGLLWIGTADG
                     GLCRFDRDKESFVI"
     gene            complement(363..761)
                     /locus_tag="PK754_16650"
     CDS             complement(363..761)
                     /locus_tag="PK754_16650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB11186.1"
                     /translation="MSVKPCILCLDDDAEILKSLKLLFENDYEVLTTTSPFQALGLLK
                     THKVNLIISDHRMPEMNGLEFFQKVKEEHGDKQYYKIIYSGYSEMEKDMIKMIASKVI
                     NEFLTKSCPTKLLYKTIASGVEKSKTMVNL"
     gene            907..1239
                     /locus_tag="PK754_16655"
     CDS             907..1239
                     /locus_tag="PK754_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HNB11187.1"
                     /translation="MKDKNSKIVYSTDPNYTDNEPQEESSSPREKNYFLKMYLDRKQR
                     AGKSVTVLEGFTESADDIAQLAKRLKNLCASGGTAKDRLIEIQGDHRDKIEKEMTKLG
                     YKIKRSGG"
     gene            complement(1339..>1578)
                     /locus_tag="PK754_16660"
     CDS             complement(1339..>1578)
                     /locus_tag="PK754_16660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11188.1"
                     /translation="LHETQTAINVLEEALWQRVIGKINPAILGHALGVISTILGAAKD
                     RLGQTYVALASNRRAPSLNLQALFVGSEGNPVSHN"
BASE COUNT          497 a          334 c          316 g          431 t
ORIGIN      
        1 tatatgacaa atgattcttt atcacgatca aaacgacaca accctccgtc ggccgtaccg
       61 atccataata aaccgctacg atctttgaaa accttgagaa tacggttatc cgagagtgac
      121 gccgtatcca aatggttgtt tttgtaaacg gtaaaattgt aaccgtcgta acgattcaga
      181 ccatcctgtg taccaaacca taaaaatcct cggctatctt gtacgatgct atatactacg
      241 gtttgcgaca acccctgctc tatcgaaagc gtctcaaacg taatgttttg ctgtgcgtgc
      301 agagctgaca accaaaacag caccgtacca ataaaaaacc gacgaatcat gtggggaagc
      361 ctttacaagt taaccatggt tttagatttt tcgactccgg aggcaatcgt tttatacaga
      421 agtttagtgg gacaagactt ggtaagaaac tcgttgataa ccttggaagc aatcattttg
      481 atcatgtctt tttccatttc ggaatagccg gagtagatga tcttatagta ttgcttatcg
      541 ccatgctctt ctttaacttt ttggaaaaac tccaagccgt tcatttcagg catgcgatgg
      601 tcgctgatga tgagattgac tttatgcgtt ttcaaaagac ccagcgcttg aaaaggactt
      661 gtggtcgtca ggacttcata atcattttca aataaaagtt tgagtgattt aagaatttcg
      721 gcatcgtcgt caagacacaa aatacagggc ttcacagaca tgagacactc cgtttgaata
      781 atatataaat gaattaatta tcggtatcag accggcttta aaagaggcag cgtttatgga
      841 ttttcttata gtgaaacgcg aacaatctaa tcaaaacaaa aggaagtctc aagaacatta
      901 atgcccatga aagataaaaa ttcaaaaatc gtgtattcta cggaccctaa ctatactgat
      961 aatgagccgc aagaagaaag ttcttcacca cgcgaaaaaa attacttcct taagatgtat
     1021 cttgatcgaa aacaacgcgc aggtaaaagt gttaccgtat tagagggatt cacagaatcc
     1081 gcggatgaca tagcacagct tgccaagcgt ctgaaaaatt tatgcgcaag cggtggcaca
     1141 gctaaagatc gtcttatcga aattcagggc gatcatcgcg acaaaatcga aaaagaaatg
     1201 actaaattag gttataaaat caaacgcagc ggtgggtgac atttaatcga aataactgtt
     1261 ttatatcata attttgttat ggcgtacgtt tgtatcactg ttcactgaaa aacattttcg
     1321 tcattttaaa gcctgcggtc aattgtgtga aaccggattg ccttcactac cgacaaacag
     1381 cgcctgcaaa ttgagtgatg gagcacggcg attgctggcc agcgctacat aagtctgacc
     1441 tagccgatct ttggccgcac caagtatggt gctaatcaca cccaacgcat gtccaagaat
     1501 agccggattg atcttcccga taactctctg ccataacgct tcctccagca cattgatcgc
     1561 cgtttgcgtt tcatgaag
//
LOCUS       DAOQNQ010000490         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_62783_length_1568_cov_4.699934, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000490 DAOQNQ010000000
VERSION     DAOQNQ010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62783_length_1568_cov_4.699934"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..72
                     /locus_tag="PK754_16665"
     tRNA            2..72
                     /locus_tag="PK754_16665"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34..36,aa:Gln,seq:ctg)
     gene            209..394
                     /locus_tag="PK754_16670"
     CDS             209..394
                     /locus_tag="PK754_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018111087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNB11189.1"
                     /translation="MNLGMPEIIVIAVAVLILFGAKKIPEFAQGLGKGIREFKRAMNG
                     AEDEIKKGLSDNQERKP"
     gene            477..>1568
                     /locus_tag="PK754_16675"
     CDS             477..>1568
                     /locus_tag="PK754_16675"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNB11190.1"
                     /translation="MHKTFSQISENLKSGATNCETIVSQYLDSIKSQSALNAFIHVLP
                     YEKTIARAKHIDAKVKSGQYGRLAGMVVAVKDAIVVKDESATAGSQILKNYVSPFSAT
                     VIERLENEDAIVIGKANMDEFAMGSSNENSSFGPALNPLDTSRVPGGSSGGSAVAVAA
                     GLSTTSLGSETGGSVRLPASFCGVVGLKPTYGRISRYGLIAFASSFDQIGPMGLTVED
                     TARLTHTIAGIDHRDATSASSPVPNYTDELKKGVKGLRIGIPKEYFGQGLDPEIRAGI
                     EKTIEKLSKEGAVFSDIALPHTEYGIATYYILVTAEASSNLARYDGVRYGHRSTKSVT
                     MADMFIKSRSEAFGEEVKRRIMLGTYVLSA"
BASE COUNT          420 a          357 c          361 g          430 t
ORIGIN      
        1 ttggaatgtc gtccaacggt aggacagtgg actctggatc cattaatcgg ggttcgaatc
       61 cctgcattcc agcatgtaaa tgcccgattt tgatcgggca tttactttta ttacctacaa
      121 taaattgctt gcaattttcg acctcgtttt ctatcataaa ctattgagtc aaatatgttc
      181 aatttatccc ttttgggagg tgttttagat gaatttagga atgcccgaaa taatcgtaat
      241 agctgtcgcc gttcttattc tcttcggcgc aaaaaaaata cccgaatttg cgcaaggtct
      301 tggcaaaggt attcgcgaat ttaagcgtgc aatgaatggt gctgaagatg aaatcaaaaa
      361 aggtttatcc gataatcagg aacgtaaacc ctaagctgat ttaacatgtt ttttcactat
      421 cccgataggc cgcatggtga tcgggatttt ttttgtataa ccatatttaa ttcacaatgc
      481 ataaaacttt ttctcagatc tctgaaaatt taaaatccgg cgcgacgaac tgcgaaacga
      541 tcgtttctca atacctcgat tcgatcaaat ctcaatccgc tttaaacgca tttatccacg
      601 ttttgccgta cgaaaaaacc atagcacgag ccaaacatat tgatgctaaa gtcaaatcgg
      661 gccagtatgg ccgtttagcc ggtatggtcg tcgccgtaaa agatgcgatc gtcgtcaaag
      721 atgaaagcgc aacggccggt tcacaaattt taaagaacta tgtctcccct ttttccgcaa
      781 cggttattga gcgccttgaa aatgaagatg caatcgtcat cggtaaagcc aatatggacg
      841 agtttgcgat ggggtcctcc aatgagaatt cttcatttgg tcccgcgctc aaccctttag
      901 atacatcgcg ggttcccggc ggttcatccg gtggcagcgc cgtcgccgta gccgcaggac
      961 ttagcaccac atcgctagga tctgaaaccg gtggatcggt tcgtttaccg gccagttttt
     1021 gtggtgtggt cgggctcaaa ccgacgtatg ggcgcatttc gcgttatggc ctgattgcgt
     1081 ttgcctcttc ctttgatcag atcgggccga tggggcttac ggtcgaagat acggcaaggc
     1141 tcacgcacac tatcgccggt attgatcacc gcgacgcgac cagcgcttct tcccccgttc
     1201 ccaattacac ggatgaatta aaaaaaggtg ttaaaggact tcgtatcggt attcccaaag
     1261 aatatttcgg acaaggactt gatcctgaaa ttcgagcagg tatcgaaaag acgattgaaa
     1321 aactgagtaa agaaggcgcc gtgtttagtg acatagcgct gccacataca gaatacggca
     1381 ttgcaacata ttatattctt gttacggcgg aggcctcttc caatcttgca cgctacgacg
     1441 gagtacgtta tggtcaccgc agtacaaaat ccgtcacgat ggcggacatg tttatcaaaa
     1501 gtcgcagtga agccttcggc gaagaggtaa agcgtcgcat catgctcggt acttacgtgt
     1561 tgagtgca
//
LOCUS       DAOQNQ010000491         1564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_63106_length_1564_cov_4.451292, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000491 DAOQNQ010000000
VERSION     DAOQNQ010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63106_length_1564_cov_4.451292"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..875
                     /locus_tag="PK754_16680"
     CDS             252..875
                     /locus_tag="PK754_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11191.1"
                     /translation="MSEEKKEHFVGRLIFELLIVFAGVYGAFVVSDFQDQQRAKRARI
                     AFLQTFREDLVRMDRQMVIPRMQVLDSLIVFYESAMAAGKKPELKFHPTIDFTSTMLV
                     VRAAFNSDYLENIEREHIVSISQGSNLITLIEKKSDVFQNECRRWFHGPSYTNDDFYD
                     RSGRLKPGMQWYLDDMKELRHLLQILDIAIMQGAVKSTDHLLKKEME"
     gene            879..1274
                     /locus_tag="PK754_16685"
     CDS             879..1274
                     /locus_tag="PK754_16685"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNB11192.1"
                     /translation="MKDILDLLREPNVTIAVVGANNNPNKYGHTIYRDLRRKGYTIFP
                     VNPSATEVEGDKAYANLAALPVKPTIINFVVPPSVTLNILQECLELGHTNVWLQPGSE
                     NDEVLNFLETRRFNYLANACIMIESRVLH"
BASE COUNT          426 a          329 c          362 g          447 t
ORIGIN      
        1 tatagcggca tgtgaatggt tcgttgacaa ataggcgatc cataaaaaaa tttttcaaaa
       61 gattttttca tttctctttg cggctttgcg cgagggaatt ggtactcgca aaagcgccaa
      121 ggcataaaaa tcatcaaagt ttttttaatt gaattggcat gggtatattg catcgaatca
      181 ttggacgccc gttttgtgtt tgtaatttgt tacgtaggcc attagattga cgacaaaatc
      241 gggagctgtg catgtccgaa gaaaaaaaag aacatttcgt aggccgatta atttttgaac
      301 tcctcatcgt atttgccgga gtatacggcg cttttgtcgt atcggatttt caggatcagc
      361 aacgcgccaa acgggcacgg atcgcatttc tgcagacgtt tcgcgaagat ctggtgcgca
      421 tggatcggca aatggttatc ccgcgcatgc aagttttgga ttcgttgatt gtgttttatg
      481 aatcggccat ggccgccgga aaaaaaccgg aattaaaatt tcaccctacg attgatttta
      541 cttccacgat gctggtagtg cgtgcggcgt tcaattcgga ctatttggaa aatatcgaac
      601 gcgagcatat cgtgagtatc agtcaaggtt ccaatctgat cacgttgatc gaaaaaaaat
      661 cggatgtttt tcaaaacgaa tgccgccggt ggtttcacgg cccgtcgtat acaaatgatg
      721 atttttatga tcgctccgga cgattaaaac cggggatgca gtggtacttg gatgatatga
      781 aagaattgcg acacttattg cagatactgg atatcgccat catgcaaggt gccgtaaaat
      841 ccacggacca tcttttgaaa aaagaaatgg agtgaccgat gaaagatatc cttgatctgc
      901 tgcgtgaacc aaacgtgacg atcgccgtcg tgggggccaa taataatccc aataaatacg
      961 gccatacgat ttatcgtgat cttcggcgta aaggttatac gatttttcct gttaatccct
     1021 cagccaccga agtcgagggg gataaagcct acgccaatct tgcggcatta cccgtaaaac
     1081 cgacgatcat caattttgtc gtccctccgt cggtaacatt aaatatccta caagagtgcc
     1141 tggaactcgg acataccaat gtatggctgc aacccggttc ggaaaacgac gaagtgctga
     1201 actttcttga aacccggcga ttcaattatt tagccaatgc gtgtattatg atcgagagcc
     1261 gcgttctgca ttgatcagtt tttcatatat taagcatctc tgattttacg tttgtttgat
     1321 ctgtagatat atggttcgat cccgattact gaaatttgta gatcaaactg gacttacaaa
     1381 attttttatg gatacttttt ccgcttctgc gcgaatgaca gtgcgggtaa ggcaaaacac
     1441 aaaccattgt cttaccgttt ccttaaaagg ttaccgttcc ggcgcaagca tgtcgttccc
     1501 gtgaaagcat gtcgttcccg cgaaagcgtg tcgttcccgc gaaagcgtgt cgttcccgcg
     1561 aaag
//
LOCUS       DAOQNQ010000492         1564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_63162_length_1564_cov_2.408880, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000492 DAOQNQ010000000
VERSION     DAOQNQ010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63162_length_1564_cov_2.408880"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="PK754_16690"
     CDS             <1..1031
                     /locus_tag="PK754_16690"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNB11193.1"
                     /translation="KKDWYFVAEDMGRTSVYNYNTQNQKIEKLFTEGSMANMNVGADK
                     LWFLRTSISSPAEIWEYNFKTKKAGAVTSFNKSLLDSLDFGKTESLTFKGADNDDVQM
                     YVVYPPNFDPNKKYPLVHMIHGGPHGAFGDDWSYRWNAHAFAAPGYVVALINFHGSTG
                     FGKSFMTSIVGAHADKPFTDIMRGTDYLLQKYNFLDSTRMAASGGSYGGYLVNWIAGH
                     TNRFKCLISHAGVYNLMGQFASDVTFDRVINYDGSPWSGRYEQINRWSPAFFAHNFST
                     PMLIIHGEKDYRVVVTQGIELYGVLTGKGVPARLLYYPNENHWVLQPQNSIYWNKEVR
                     DWFARWLK"
     gene            1102..>1564
                     /locus_tag="PK754_16695"
     CDS             1102..>1564
                     /locus_tag="PK754_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNB11194.1"
                     /translation="MLPLLTNEELSQLPPDGGSEYNRLIFEKSPYLLQHARNPVKWWS
                     WCDEAFETARAEDKPVFLSIGYATCHWCHVMEHESFEDAEIASAMNALFICVKVDREE
                     RPDIDHVYMAVTQAMTGHGGWPMTVLMTWDKKPFFTGTYFPKRSRHGRIGMH"
BASE COUNT          431 a          355 c          349 g          429 t
ORIGIN      
        1 ataaaaaaga ctggtacttt gtagcggaag acatggggcg tacgtccgta tataactaca
       61 atacgcaaaa ccagaaaatt gaaaaattat tcaccgaagg cagcatggcc aatatgaatg
      121 tgggtgccga caaactttgg ttcttacgca caagtatttc atcccctgct gaaatttggg
      181 aatataattt taaaaccaaa aaagccgggg cggtaacgtc gtttaacaaa tcgctcttag
      241 attctttgga ttttggcaaa acggaatccc tcactttcaa aggcgcagac aacgacgatg
      301 tacagatgta tgtagtttac cccccaaatt ttgatccgaa caaaaaatat ccactagttc
      361 atatgataca cggcggcccg catggcgcct ttggcgatga ctggagttac cgctggaatg
      421 cacatgcttt tgcggcgccg ggctacgtcg ttgctttgat caattttcac ggctcgaccg
      481 gcttcggtaa atcttttatg acatccatcg tcggtgctca tgccgataaa ccgtttaccg
      541 atattatgcg cggcacggat tatttgctgc aaaaatacaa ctttctcgac agcacacgta
      601 tggcggccag cggcggaagt tacgggggct atttagtcaa ttggattgcc ggccatacca
      661 atcgttttaa atgcctcatc agccatgccg gtgtatataa cttgatgggc cagtttgctt
      721 ctgacgtgac gtttgatcgt gtgatcaatt atgacggctc accatggtcc ggtcgttacg
      781 agcaaatcaa ccgctggagt ccggcttttt ttgctcataa tttttcaaca cctatgttga
      841 ttattcatgg cgaaaaagat taccgcgtcg tcgtcacaca aggcattgaa ctatatggtg
      901 tacttacggg taaaggcgtt cccgcacgtc tcttatacta ccctaacgaa aatcattggg
      961 ttcttcagcc tcaaaattcc atttattgga ataaagaagt gcgcgattgg ttcgcacgtt
     1021 ggttaaaata aacatttcat ttaatcatag cacaaaaggt caaacgatta cgtttgacct
     1081 ttattttttt acataatcac tatgctgcct ttacttacaa acgaagaatt atcccaactg
     1141 cctccggacg gcggctctga atataaccgg cttatttttg aaaaaagccc ctatctgctc
     1201 caacacgcac gtaatcctgt aaagtggtgg tcatggtgtg acgaagcgtt tgaaacagca
     1261 cgcgccgaag ataaacctgt ttttttatcc atcggttatg cgacatgcca ctggtgccat
     1321 gtgatggaac atgaatcgtt tgaagatgcc gaaattgcct cagcgatgaa cgcattgttt
     1381 atctgcgtca aagtagatcg cgaagaaagg cccgacattg accatgtcta tatggccgtc
     1441 acgcaagcca tgacaggtca tggcggatgg ccgatgacgg tattgatgac atgggataaa
     1501 aaaccttttt ttacgggaac ttactttcct aaacgtagcc ggcacggccg catcggcatg
     1561 catg
//
LOCUS       DAOQNQ010000493         1554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_63996_length_1554_cov_6.589059, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000493 DAOQNQ010000000
VERSION     DAOQNQ010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63996_length_1554_cov_6.589059"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="PK754_16700"
     CDS             <1..1160
                     /locus_tag="PK754_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HNB11195.1"
                     /translation="ALGFPFNLVAVGFEKIGAGIVAILGYTTKGAQFVFGNLALSPGM
                     EGSMGFFFVFQVLPTIIFFSSLMSVLYYLGIMQKVVQGMAWVMAKLMGTSGAESLSNT
                     ANIFVGQTEAPLMIRPFIKTMTNSELYTIMVGGMATIAGGVMAAYIQMLGKSYADVHG
                     VGVEQGQILFATQLLGASIMAAPAGLLISKIIFPEVEEPVTKGSVKMDVEKNASNVIE
                     AAAGGAADGLQLALNVAGMLLAFIALIALINGLAGWVGGLIAIPDLSLQMIFGYILAP
                     IAFISGVPWSEAMQVGSLLGTKIALNEFVAYSDMAGMIKSGLLTSDKAIMIATFALCG
                     FANFSSIAIQIGGISPLAPERRKDIAALGLKAVLGGALATLLTGTIGGILL"
     gene            1174..>1554
                     /locus_tag="PK754_16705"
     CDS             1174..>1554
                     /locus_tag="PK754_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11196.1"
                     /translation="MNLEKWRRYKIHVTAFLLVFILTAVFGAPKLAHYMIRTKISETA
                     DRFAARTGYRVMIDSLNFPTASRLTVQSLVLIGDSSHTSGYKELLRLERVTLDFYLWG
                     FLTGNYIRALQMDSVHIVSTRSLAG"
BASE COUNT          357 a          329 c          411 g          457 t
ORIGIN      
        1 atgctttagg atttccgttt aatctcgtag ccgtaggatt tgaaaaaata ggcgcgggta
       61 ttgttgcaat tttgggttat acgacgaaag gtgcacaatt tgtttttggg aaccttgcgt
      121 taagcccggg aatggaaggc agcatgggtt tcttttttgt gtttcaggtt ttgcccacga
      181 ttattttctt ttcgtcgtta atgtcggttc tatattatct cggtatcatg caaaaagtag
      241 tacaaggtat ggcttgggta atggccaaac tgatgggcac gagcggagcg gaatcgcttt
      301 ccaatacggc aaatattttt gtcggtcaaa ccgaagcgcc tctgatgatt cgaccattta
      361 tcaagaccat gaccaattca gaactgtaca ccatcatggt cgggggtatg gcgaccattg
      421 ccggcggtgt gatggcggcc tatatccaga tgctgggtaa atcgtatgcc gatgtgcacg
      481 gcgtgggtgt cgaacagggt cagatattat ttgcgacgca gcttctcggt gcaagtatta
      541 tggccgctcc ggccggttta ttgatttcta aaattatttt cccggaagtg gaggagcctg
      601 tgacgaaagg ttccgtcaaa atggatgttg aaaaaaatgc atccaatgtg atcgaagccg
      661 cggccggcgg cgccgctgac gggttgcaac tggcgctcaa tgttgccggg atgcttttag
      721 ctttcatcgc gttgatcgca ttgatcaatg gtttagccgg atgggtcggc gggctcattg
      781 cgattccgga tctatccctg caaatgattt ttggatatat tcttgcgccg atcgcgttta
      841 tttccggtgt gccgtggagc gaagccatgc aggtcggcag tttacttgga acgaaaatcg
      901 cattaaacga atttgtcgct tactccgata tggccggcat gatcaaaagc gggctgctta
      961 cttcggataa ggccatcatg atagctacgt ttgcgttatg cggctttgcc aatttttctt
     1021 caattgccat tcagatcggc ggtatatcac cattggcgcc ggagcgtcga aaagatattg
     1081 cggccctagg actaaaggct gtgctcggcg gcgcgttagc gacattatta accggaacga
     1141 tcggcggaat tttgctctga agaaagtgta ctcatgaatt tggaaaaatg gcgccgttat
     1201 aaaatacatg taacggcgtt tcttttagta tttattctca ccgccgtgtt cggagcgccc
     1261 aaacttgcgc attatatgat ccgaactaaa atatccgaaa cggcggatcg cttcgctgca
     1321 cgtacgggtt accgtgtaat gatcgacagt cttaactttc cgacagcctc tcgattaacc
     1381 gtgcagtcgc ttgtactgat cggtgattca tcgcatactt ccggttataa agaattgctg
     1441 cgattagagc gagtgacatt ggatttttat ctttggggat ttctcaccgg taattatatc
     1501 cgtgcgttgc aaatggactc ggttcatatt gtttctacac gcagtttggc cggt
//
LOCUS       DAOQNQ010000494         1535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_65711_length_1535_cov_4.492568, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000494 DAOQNQ010000000
VERSION     DAOQNQ010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65711_length_1535_cov_4.492568"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PK754_16710"
     CDS             <1..235
                     /locus_tag="PK754_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11197.1"
                     /translation="VRKLGFTDIQLVKDREISLPDDILAPYLNKEEIAEFRKSGARVV
                     SITVVAQKSTQVQTGCCSDGNVAKSKAGGCGCN"
     gene            complement(382..750)
                     /locus_tag="PK754_16715"
     CDS             complement(382..750)
                     /locus_tag="PK754_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11198.1"
                     /translation="MPRITFNFGWLLFAIGIAGYVASGMSSTTALIPSIFGIAFVLLG
                     WIAMQKESMRKHLMHAAAILGLLGIIGTFKGMLRFFAMLGGTEVERPIAVTFQSVMAM
                     LCIVFVALCVHSFIKARTGK"
     gene            complement(759..>1535)
                     /locus_tag="PK754_16720"
     CDS             complement(759..>1535)
                     /locus_tag="PK754_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004778917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNB11199.1"
                     /translation="QQLDNNVKRLEQVLDGLYELALGGTAVGTGLNTHPKFAETGAKH
                     IAQITGLPFRSAPNKFEALAAHDAIVFASGATKTLAASLMKIANDVRWLASGPRCGIG
                     EISIPDNEPGSSIMPGKVNPTQCEAMTMVCAQVMGNDAAINVAGASGNFELNVYKPVL
                     IFNLLNSIRLLGDACDSFNEHCAVGIEPNTNVIKKHLDNSLMLVTALNPHIGYDNAAK
                     VAKKAFHDNITLKEAAIQLGLLTSEKFDEVVRPEKMINPS"
BASE COUNT          451 a          351 c          318 g          415 t
ORIGIN      
        1 cgtccgtaaa ctcggtttca cggatattca gttagtcaag gatcgcgaaa taagcttgcc
       61 ggatgatatt ttggctccgt atctgaataa agaagaaatt gctgaatttc gtaaatccgg
      121 tgcgcgcgtc gtaagcatca ctgtagtagc acaaaaaagt actcaagtgc aaaccggttg
      181 ttgctcggat ggcaatgtag ccaaatccaa agccggcggt tgcggctgta attaaaaaaa
      241 aagaaaaagg ctgtttgacg tgagctctca tatttaacga tgagaaactt ttattcacat
      301 aaaattgacg attcgttatt aaaaacgatc ttcgcaaggt tttgatgtga actgtcaaac
      361 agccttttct atttctacaa tctacttacc tgtgcgtgct ttgataaaag aatgtacaca
      421 caaggctaca aatacaatac aaagcatagc cataactgat tgaaatgtca cggctatagg
      481 cctttcaact tccgtaccgc ctaacattgc aaaaaatcta agcatgcctt tgaatgtgcc
      541 gataatgccc aataaaccca gtattgcggc ggcgtgcata agatgtttac gcatggattc
      601 tttttgcatg gctatccaac ctaagagcac aaacgcaatt ccgaaaatag acggaataag
      661 cgccgtcgtg ctggacatac cgctagcgac ataccctgct ataccgatcg caaaaagcaa
      721 ccaaccaaaa ttgaaagtga tacgaggcat aaatctcctt aggatgggtt aatcattttt
      781 tcgggacgaa caacttcatc aaatttttct gacgttaaca aacccaattg aatcgccgct
      841 tctttgaggg tgatattatc atgaaacgcc ttcttagcga ctttggctgc attatcataa
      901 ccgatatgtg gatttaaagc cgtgactaac atgagggagt tatctaaatg ttttttgatc
      961 acattggtgt taggttcaat accgacagca caatgttcat taaacgaatc gcaggcgtca
     1021 ccaagtaaac gaattgaatt cagaagatta aaaatcagta ccggtttata tacgtttagt
     1081 tcaaaattac ctgaagcgcc ggcaacattg attgcggcat cattacccat cacctgcgca
     1141 cacaccatgg tcatggcttc gcactgcgta ggattgactt taccgggcat gatggaactt
     1201 ccgggttcat tatccggtat agatatttca ccgatcccac aacgcgggcc ggaagccagc
     1261 caccgtacgt cattggctat tttcatcagc gatgccgcca atgttttagt tgcgccgctc
     1321 gcaaacacga tcgcatcatg cgccgctaat gcttcaaact tattcggcgc actgcgaaac
     1381 ggcagacccg taatttgtgc gatatgtttt gcaccggttt ccgcaaactt aggatgcgta
     1441 ttaaggcctg ttccgaccgc cgtaccaccc aaagccagtt cgtataatcc gtcgagtacc
     1501 tgctcaagtc gcttgacatt gttatctaat tgttg
//
LOCUS       DAOQNQ010000495         1531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_66058_length_1531_cov_4.262195, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000495 DAOQNQ010000000
VERSION     DAOQNQ010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66058_length_1531_cov_4.262195"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..184
                     /locus_tag="PK754_16725"
     CDS             17..184
                     /locus_tag="PK754_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HNB11200.1"
                     /translation="MGMINKFAIFGQLLQFLWEKKLWWMIPMVIVLVLFGLLLVFAQG
                     SGLAPFIYTLF"
     gene            192..>1531
                     /gene="asnB"
                     /locus_tag="PK754_16730"
     CDS             192..>1531
                     /gene="asnB"
                     /locus_tag="PK754_16730"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNB11201.1"
                     /translation="MCGIVGFISAENLDRPSLLKAMTDVIAHRGPDDDGTFFDKKAAL
                     GFRRLSIIDVAGGHQPLSNTDQSLWIVFNGEIYNYKEVRTELIAKGYSFKTNSDTEVI
                     IHAYQAWGSRCLQKLNGMFAFAIWDTQRERLFIARDRLGVKPLYYWHSGSQFVFGSEI
                     KSLLKFPDAPRQVDPEAINAYMSFLWIPDPHTGFKNIYKLPPGHFAVLENSTLRTECY
                     WDLTFDPQPNISDAQWYDEIRHHLARSVKDRLISEVPLGAFLSGGIDSTAIVAMMARM
                     TDKKISTYTIGFPPEDMKDDVIGSDLEFARLASQKFPIDYHEIIVRPDVADLLPKLVW
                     HMDEPLADPAAITTYLVCKASRETLTVLLSGVGGDEVFGGYPRYLAARMASWYEYIPG
                     FMRKGIISPLIQTLPGAQSALFRNLKKFDRSSTLPFRERYMGYRTYFSEEEKKS"
BASE COUNT          404 a          321 c          345 g          461 t
ORIGIN      
        1 tgtaaggaat aaaaacatgg gcatgattaa taaattcgca atatttggcc agctattgca
       61 gttcttatgg gaaaagaagt tgtggtggat gataccgatg gttatagtac ttgtgctttt
      121 tggactgctg ctcgtatttg ctcaaggctc aggattggcg ccgtttattt atacattatt
      181 ttaatatcac tatgtgcgga attgttggct ttatcagcgc tgagaatttg gatcgtccat
      241 ctcttttgaa agcgatgacg gatgttattg ctcatcgcgg accggatgac gacggcactt
      301 tttttgataa aaaagcggcg ctgggttttc gtcggttatc tattatcgat gttgccggcg
      361 gtcaccaacc tctttccaat accgaccaat ctctttggat tgttttcaac ggcgaaatat
      421 ataattataa agaagtccgt accgagttga ttgctaaagg gtattcattc aaaaccaatt
      481 ctgataccga agttatcatt catgcatatc aagcgtgggg ttcccgctgt ctacaaaaac
      541 tgaatgggat gtttgcattt gcgatctggg atacacaacg agaacgactc tttatagcac
      601 gtgacagatt aggcgtaaaa cctttgtact attggcattc gggttcgcaa tttgtatttg
      661 gctcggaaat aaaatcactt ttaaagtttc ccgatgcacc ccgtcaagta gatcctgagg
      721 ctatcaacgc ctacatgtca ttcctatgga taccagatcc gcatactggt tttaagaaca
      781 tttataaact tcccccaggg cattttgccg ttttggaaaa ttctacattg cgtacagaat
      841 gttattggga tcttacattt gatcctcagc cgaatatatc cgacgcacaa tggtacgatg
      901 aaatccggca tcatttggca cgttctgtca aagatcgctt gatcagcgaa gtaccacttg
      961 gcgcttttct cagcggtggt attgattcga ctgcaattgt agctatgatg gcacgcatga
     1021 ctgacaaaaa aatcagcacg tatactatcg gatttccacc cgaggatatg aaagacgatg
     1081 ttataggaag cgacttggaa tttgcccgat tagcttctca aaaattccct atcgattatc
     1141 atgagatcat tgttcgtccc gatgtggctg atctgctccc aaaactggtt tggcacatgg
     1201 atgaaccgct tgcagatcct gcagctatta ccacatacct cgtatgcaaa gcatcacgcg
     1261 aaaccttgac ggtcttactc agcggggtgg gaggagatga ggtattcggc gggtatccgc
     1321 gataccttgc agcgcgcatg gcttcgtggt atgaatacat tcccggtttt atgcgcaaag
     1381 ggatcatttc acctttgatt caaacgctac ccggcgcaca gtccgcgtta tttcgtaatc
     1441 tcaagaaatt tgaccgttct tcgacgttgc catttagaga gcgttatatg gggtaccgta
     1501 cgtatttttc tgaagaagaa aagaaatcat t
//
LOCUS       DAOQNQ010000496         1524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_66645_length_1524_cov_4.559564, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000496 DAOQNQ010000000
VERSION     DAOQNQ010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66645_length_1524_cov_4.559564"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PK754_16735"
     CDS             <1..256
                     /locus_tag="PK754_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11202.1"
                     /translation="DTLGTKLSGGHVMGYQPWTMGGQIVLTKRALRSINRCMEILFTV
                     KDQWALDYDQYLQLDEIGLETRNTIALRLQDLREQFKSSF"
     gene            complement(240..>1524)
                     /locus_tag="PK754_16740"
     CDS             complement(240..>1524)
                     /locus_tag="PK754_16740"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB11203.1"
                     /translation="RAWMQWIFSLCQHFPLLVLYSVVLFILSWKITLLSLVLIPGITL
                     LQHRLQKYTARWTLFLHERESEIQSDVWHKLQAFKLIRLFHSAGYEAKRFSSLESRRS
                     DAATRRDRLQAIVLATVEMAGVSVGVMLLLTIGSWTMEGEFMYGPGGFVLFIAAAFSI
                     IDPARHIARGRMFLSEARTLWDEYKNKPMASSEETSPTKDIASFTNALCLEETCFAHR
                     TTEILNKVNITIRRGECVIITGKTGAGKSTILDLIIGLYAPQRGCITLDGIPVTQISH
                     ATRAKLFGLMTQEPLIMHATVRDNVVYDQIHVSDEHVIEALTHVQLDTWIKTLPDGLD
                     TIIGEKGWTMSGGERQRLALARIVLRKPEILLLDEATSALDETTEKALLETMARLFSD
                     KTRIIVSHRPAARRWADRMFELDEGQLIEISKKTI"
BASE COUNT          398 a          382 c          352 g          392 t
ORIGIN      
        1 cgacacgctc ggcaccaaac tttccggcgg ccatgtgatg ggataccagc cgtggacgat
       61 gggcggtcaa atcgtattga ccaaacgcgc attacgttcg atcaatcgct gtatggaaat
      121 tctttttacc gtcaaggatc agtgggcgct ggattacgat cagtatctgc agctagacga
      181 aatcggcctc gaaacacgta ataccatcgc tttgcgtctt caggacctgc gtgaacaatt
      241 caaatcgtct ttttagaaat ttcaatcaat tgtccctcat ccagttcaaa catccgatct
      301 gcccaccggc gcgcggccgg gcggtgagag acgatgatgc gcgttttatc cgaaaataaa
      361 cgggccatcg tttcgagaag cgctttttca gtcgtctcat ccaaagccga tgtggcttca
      421 tccaaaagta aaatttccgg tttacgcaaa acaatacgtg ccaaagctag ccgctgacgc
      481 tcaccgccgg acatggtcca tcctttttct ccgatgatgg tatccaagcc atcaggtaat
      541 gttttaatcc atgtgtccag ttgaacatgc gtgagcgctt cgatcacatg ttcatcgctg
      601 acatgaattt gatcgtacac gacattgtct cttaccgtcg catgcatgat cagcggttcc
      661 tgtgtcatga gtccgaaaag ttttgcacgt gtagcgtgcg aaatttgtgt tactggaatg
      721 ccgtcaagcg tgatacagcc gcgttggggt gcatacaacc cgatgatcaa atccaaaata
      781 gtgcttttac cggcgccggt tttgcctgtg ataataacgc actcgcccct acgaatagtt
      841 atatttactt tgtttaaaat ttcggtggtg cgatgtgcaa aacaagtttc ttccaaacaa
      901 agtgcgttcg tgaatgaagc gatatctttc gtcggtgatg tttcttcgga cgacgccatc
      961 ggtttgtttt tgtattcgtc ccacaacgta cgggcttcgg ataaaaacat acgtccgcgt
     1021 gcgatatgcc gcgccggatc aatgatcgaa aacgcggcgg cgataaataa tacaaaaccg
     1081 ccgggcccat acataaattc gccttccatc gtccatgagc caatcgtcaa taggagcatt
     1141 acacccacgc tcacacccgc catttctacc gtggccagta cgatcgcttg caaccggtca
     1201 cgacgggttg ccgcatcgct ccgacggctt tccagtgatg aaaaacgctt cgcttcgtac
     1261 ccggcgctgt gaaatagtcg aatcaatttg aaagcttgca gcttatgcca gacatccgac
     1321 tggatttcgg attcgcgctc gtgtaaaaaa agcgtccatc gtgctgtgta tttttgtaag
     1381 cgatgttgca ataacgtaat acccggaatc aatacaaggc tcaataaggt gatcttccag
     1441 ctgagtataa aaagcactac tgaatagagt acaagtaaag gaaaatgctg gcaaagagaa
     1501 aaaatccatt gcatccatgc gcgt
//
LOCUS       DAOQNQ010000497         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_67252_length_1517_cov_5.139535, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000497 DAOQNQ010000000
VERSION     DAOQNQ010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67252_length_1517_cov_5.139535"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            448..1335
                     /locus_tag="PK754_16745"
     CDS             448..1335
                     /locus_tag="PK754_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HNB11204.1"
                     /translation="MSEIHIDALLPAEMATRAEYLGVRKAEMPALKMFTLAIMAGAFI
                     SLGAIFATTVSAGGVAITAPDGATSLSTGLPYGVTRLLAGFVFCLGLILVVVGGAELF
                     TGNNLIVMAWASRKVTSYALLRNWAIVYAGNLIGALGTAVLMLWSRQYTFGSNSIGLS
                     AFKIAAGKCSLEFWQAMVLGILCNALVCLAVWLTYSARSTMDKIAAIIFPVTAFVAAG
                     FEHSIANMYFVPYVLLIQTFDPSYVASISDKMPGMYNLNWPNFITNNLIPVTIGNVIG
                     GAVLVAAVYWSVFLRNKDK"
BASE COUNT          343 a          335 c          403 g          436 t
ORIGIN      
        1 ggctcagatc gctgcgtttt tcagcaaagc caaaacgtca tcgctggtgc cggtcgcgta
       61 cacggaacga aagtatcttc gcaaaccaaa aggcgccaaa cccggcatgg ttgtgcttga
      121 acgggaagaa gttttaatgg tgccgccggt ggagccccgc gaaatggccg tctgatttat
      181 ggcggcctaa gcgaatattt ttttgtaaaa tctctgttag ttttatccct ccgattgtat
      241 taaattgaag tgcttcttta aaagaccaca agcctgctat gcagcgcgtt attcaaatat
      301 ttcccgatca gccgtcaccc ctatccagcg acgctattga aaaatacgta cgctgctgca
      361 ccgtcgcgtt ttttagtcgc ggtaagttgc ggtatgtttg tccgcatacc gacggggttt
      421 attacatttt acagtggaga aatatctatg agcgaaattc acattgatgc tttgttgccg
      481 gcggagatgg ctacacgcgc cgaatatctc ggtgtacgaa aagctgaaat gccggctttg
      541 aaaatgttta ccttggccat catggccggg gcgtttattt cgttgggggc gatctttgca
      601 acgacagtct ctgccggtgg tgtggcaatc acggcgccgg acggagcgac ttcgcttagt
      661 accggactgc cttacggcgt tacacgatta ttggccgggt ttgttttttg tctcggcctg
      721 attctggttg tcgttggcgg ggctgaactt tttaccggaa acaatctcat cgtcatggcg
      781 tgggccagcc gcaaagtgac gtcgtatgca ctgttgcgca attgggctat cgtatacgca
      841 ggaaacctta tcggcgcatt gggtacggcc gttcttatgt tgtggagccg ccaatatacc
      901 tttggttcca acagcatcgg tctatcggct tttaaaatcg ccgccgggaa atgcagtttg
      961 gaattttggc aagcgatggt attgggcatt ctgtgcaacg cgctcgtctg ccttgcggtg
     1021 tggctcactt atagcgcgcg cagtacgatg gataaaatcg cggccattat ttttccggtt
     1081 acggcctttg ttgcagcggg ttttgaacac agcatcgcta atatgtattt tgttccctat
     1141 gtgcttttga ttcaaacatt cgatccgtcc tatgtcgcaa gcatttcgga taaaatgccg
     1201 ggcatgtaca atctcaattg gccgaatttt attacgaata acctcatacc ggtgacgatc
     1261 ggcaatgtga tcgggggagc tgttttagtt gcggcggtgt attggtcggt ttttttacgt
     1321 aataaagata agtgaatggg gttggcttag agggaaccat tgcgcttacc gtgatgttgt
     1381 ccattcaaat cataaggagt atgtatgaaa gcgtatgttc tcatcaaaat aaaatccggc
     1441 gaagtcaaag atgtagtacg tcagcttcgc cgaatcgaag gcgtgatgga agcgcatatg
     1501 acctttggtc cgtatga
//
LOCUS       DAOQNQ010000498         1509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_68013_length_1509_cov_4.549519, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000498 DAOQNQ010000000
VERSION     DAOQNQ010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68013_length_1509_cov_4.549519"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PK754_16750"
     CDS             complement(<1..276)
                     /locus_tag="PK754_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086597.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HNB11205.1"
                     /translation="MSEISKCPFHAAGRGTSNQDWWPNQLNIKILHQNSSLSNPMGKK
                     FDYAKEFKKLNLKAIKKDLYALMTDSQEWWPADYGHYGGLFIRMAWHS"
     gene            450..1403
                     /locus_tag="PK754_16755"
     CDS             450..1403
                     /locus_tag="PK754_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HNB11206.1"
                     /translation="MTLLQLTYIVAVEKYRSFSKAAKDRFVTQPTLSMQIQKLEEELG
                     VTIFDRGKKPVAPTDIGRTIIEQAQAILRESDKIIQSVESETTEVAGIFRIGIIPTLA
                     PYLLNLFLQDFITKHPRVELVIEELQTREITERLNRSDLDAGLLATPLHHKNLAEKPL
                     FYEPFVAYVSEEHRLSAKKKIHIKDLQNGDLWLLTEGHCFRSQALALCKQKKNITPKH
                     KGALRFESGNLDTLRKLVEQNYGMTLLPYLAVSELSDEKKKLYMRPFAPPAPKREISL
                     VYNRSYPKKKILEVLEHAIRFSVPEEILEKDRSLIVHHGAV"
BASE COUNT          447 a          337 c          317 g          408 t
ORIGIN      
        1 gctatgccag gccatacgaa taaacaaacc gccgtaatga ccatagtccg ccggccacca
       61 ctcttgcgaa tccgtcatga gcgcatacaa atcttttttg atggctttga gattgagttt
      121 tttaaactct ttggcgtaat caaatttttt acccatagga ttggacaaag acgaattctg
      181 atgcagaatt ttgatattca actggttggg ccaccagtcc tgattggatg ttccgcggcc
      241 agccgcatgg aacggacact tgctgatttc actcataacc tttttctccc tgtttttaga
      301 tgaattcgta ggacgatttt aatgttcacg atttaaattg gcgaactgat tatatcgagc
      361 gtttttatgt cttaaagata cctatttgat ttcataagtc aaattgatta ttcttattta
      421 ataatagata atatctatat tgaataagaa tgacactact gcaactcacc tatattgtcg
      481 ctgtcgaaaa gtaccgcagt ttttccaaag cggctaaaga tcgttttgtc acacaaccga
      541 ctctgagcat gcagattcag aaattggaag aagaattagg cgtaacgata tttgaccgag
      601 gaaagaaacc cgtagccccg accgacatcg gtcgtacgat catcgagcaa gcccaagcga
      661 ttttgcgcga aagcgataaa atcatccagt ccgtggaatc cgaaaccacc gaagttgcag
      721 ggatcttccg catcggtatt atccccacgc ttgcgcctta tttgctcaat ctttttctgc
      781 aggattttat cacaaaacat ccgcgcgtag aattggtcat tgaggaattg cagacgcggg
      841 agatcaccga acgattgaac cgaagcgacc ttgatgcagg gcttttggct acgccgctgc
      901 accataagaa cttagccgaa aaaccgttgt tctacgaacc tttcgtcgca tatgtatccg
      961 aagaacaccg actaagcgcc aagaaaaaaa ttcacatcaa agatctgcaa aacggcgacc
     1021 tgtggcttct gaccgaaggg cattgttttc gcagtcaggc gctcgcacta tgcaaacaaa
     1081 agaaaaacat aacacctaaa cacaaaggtg cattgcgctt cgaaagcggt aatctggata
     1141 cgcttagaaa attagtggaa caaaattatg gtatgacact gttgccgtat ttagccgttt
     1201 cggaattatc cgatgaaaag aagaaactgt acatgcgccc ttttgcgcca ccggctccca
     1261 aacgggagat cagtttggtg tacaatcgtt cttaccctaa aaagaaaatt ctcgaagtgt
     1321 tagagcacgc catccgcttc tccgtaccgg aagaaatatt agaaaaagat agaagtttga
     1381 tcgtacatca tggtgccgtt tagagaatcc attagcgcaa taactcttcc aagagctttt
     1441 ctttattgac ggttccgata tgcgaccatg taagtcgacg gtttttgtat atgtgaaacg
     1501 tcggcacag
//
LOCUS       DAOQNQ010000499         1476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_71172_length_1476_cov_3.441942, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000499 DAOQNQ010000000
VERSION     DAOQNQ010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71172_length_1476_cov_3.441942"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PK754_16760"
     CDS             <1..292
                     /locus_tag="PK754_16760"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HNB11207.1"
                     /translation="RVHDVHRGQRPELHRQGARVVYLTPMRGVNIGAIINACRAQSIL
                     SVSGVYDFAQFGATISFDLLDNKPKFIINTTSAKLEQVVFSSDLLKLATVIE"
     gene            complement(295..1284)
                     /locus_tag="PK754_16765"
     CDS             complement(295..1284)
                     /locus_tag="PK754_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006624090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNB11208.1"
                     /translation="MPVDLSTTYMGYKLRNPVVPSASPLSKHVDNIRKMEDAGASAVV
                     LFSLFEEQIYHDFMQLVQENKKTSNDYLHSFNYLSELNQSHYNPDAYLEHIRKSKEAV
                     DIPIIASLNGSSDNGWLQYAKSIEVAGADGIELNLYHIAADPKKSSAQIEDQFIHVVR
                     SVKNLKIPVSVKLSTHITGISHFISRLDEIRIDGIALFNRFYQPDFNLNSLKVVPNIN
                     LSDSTDLRPALRWTAILYGQIQTPIAATGGIHSHEDVIKMIMAGASVTMVCSALLKNG
                     IGHIADLLRDIEMWMEIQKCDSLNHIRGSMSQKSHDDPSAIERAQYIKALAQY"
BASE COUNT          388 a          289 c          353 g          446 t
ORIGIN      
        1 ccgagtccat gacgtacacc gtgggcaacg cccggaactt catcgccaag gtgcgcgtgt
       61 tgtctatttg acgccgatgc gcggagttaa tataggagct attataaacg catgccgcgc
      121 tcaaagtata ttatcagtca gcggggtcta tgattttgca caattcggcg ctactatcag
      181 ttttgatctt ttggataaca aaccaaaatt tattatcaat acgacatcag cgaagttgga
      241 gcaagttgta ttcagttctg atctgcttaa gttagctaca gtgattgaat gaatttagta
      301 ttgtgctagc gctttgatgt attgtgcgcg ttcgatggcc gaagggtcat cgtgcgattt
      361 ttggctcata cttccgcgta tgtgattgag tgaatcgcat ttttgaattt ccatccacat
      421 ctctatgtca cgcaacaaat ccgcaatatg accgataccg tttttaagta gtgccgaaca
      481 taccatcgtg accgacgcac cggccataat catcttgatg acgtcttcat gtgagtgaat
      541 gcctcccgtt gcggcaatcg gcgtttgtat ttgtccgtac agaatagcag tccaccgcag
      601 agccggacgc aaatccgtcg aatcactgag attgatattg gggacgactt tcagcgaatt
      661 aagattaaaa tccggttgat agaaccggtt gaacaaagca atgccgtcaa ttcgtatctc
      721 atccagccgg ctaataaaat gcgatatacc agtaatgtgt gtactgagtt tgaccgaaac
      781 ggggattttt agatttttta cagaacgcac gacatggata aactgatctt cgatctgtgc
      841 tgaagatttt ttgggatcgg cggctatatg atacaaattc agttcgatac cgtcggctcc
      901 ggccacttct atagattttg cgtattgcag ccagccatta tcggatgaac cgttgaggct
      961 ggcgatgatg ggaatatcaa cagcttcttt ggatttacga atatgttcta aataagcatc
     1021 gggattgtaa tgcgattgat taagttcact cagatagtta aaagaatgca gataatcgtt
     1081 ggaagttttt ttgttttcct gtacgagttg catgaaatca tgataaatct gttcttcaaa
     1141 cagagaaaac aaaacaacgg cggaggcgcc tgcgtcttcc atttttcgga tgttgtcaac
     1201 gtgtttggac aaaggcgaag ctgaaggtac aaccggattg cgcagtttgt accccatgta
     1261 tgtggtgctg agatcaaccg gcataatgga acctttagaa tggtttggac gtgctaatat
     1321 taataatctt tgtttccaga aacaagagtc tttgcggtct tgattttggt gggatttttt
     1381 acgatgattc ggcgttataa tatatgtcat atgttctttt tcaatatggg gacgaactgg
     1441 attcgacggg tatagggtgc tttgggtcgc atgccg
//
LOCUS       DAOQNQ010000500         1457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_73126_length_1457_cov_3.239658, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000500 DAOQNQ010000000
VERSION     DAOQNQ010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73126_length_1457_cov_3.239658"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1457
                     /locus_tag="PK754_16770"
     CDS             <1..>1457
                     /locus_tag="PK754_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11209.1"
                     /translation="SNVKGPSREFMPPDIKGQNRKYVFSCWVKTQSGFGSNNGRLVIH
                     SKRNADTAKTVYPNVPGAIATIYISDTQGQWKYIEAMIDLGKVRTDGGIGSGELIRLR
                     DTISNNDATKYFLVDDIRFSPYDSPFSGIAYEKTYGLPVTSVGSNGEFSKTMYDRFQR
                     NIGTIVNDQFVPGTQMMYYSRKGNADTLNNNDLNAAVSVTASGAGKMDDFADGTADGW
                     SLGTGIWTVTSGELTCTGSGANDYATLTGTGSAGVRAKVRSVNGGENYGIQIGNVLFT
                     RYSGAWKITIGSYSQTTSAMPSGTDWLAIVRDSVSLLYVDGKQIFNVRKWNTGGNVSF
                     YVGSFNTVAGFDDFLMFRDPGVALSYLDGSGKVVQSQFLDSVGTVVSATLYDPLNRDA
                     IVTKSAKYDSMFAFRKSFVSGFNWSSGIMTGYITTTGNAGSYPYSRLKFEDSPLSGVI
                     ESSAPGDTFRIGRKTVKSAYGSNVTGDGFRDDLPA"
BASE COUNT          377 a          323 c          378 g          379 t
ORIGIN      
        1 gtagtaatgt caaaggaccg tcgcgcgaat ttatgccgcc ggatatcaaa ggccaaaatc
       61 gcaaatatgt tttttcatgt tgggtaaaga cacagtccgg attcggatca aataacggaa
      121 gattagtcat tcactccaaa cgtaatgccg acacggctaa aaccgtttat cccaacgtgc
      181 ccggagcgat agccacgata tatatcagcg atacccaagg ccaatggaaa tacatcgagg
      241 ccatgatcga tctcggaaaa gtgcgtacgg acggcggtat cggatcgggc gaattgatca
      301 ggttacggga tacgatttct aataacgatg ctactaaata ttttctggtg gatgacatcc
      361 gtttttctcc ttatgactct ccgttttcag gcattgctta cgaaaaaacc tacggacttc
      421 ctgtgacgtc tgtcggatcc aatggcgaat tctcgaaaac gatgtatgat cggtttcaaa
      481 ggaatatcgg tacgatagtc aatgaccagt ttgtgccggg tactcagatg atgtattact
      541 cccgaaaagg gaacgccgat acgctcaata acaatgacct caatgccgcc gtatccgtga
      601 cggcgagcgg ggcaggaaag atggatgact tcgctgacgg tacggcggac gggtggtctc
      661 tgggaacggg tatatggacg gtgaccagcg gggaactcac ttgtaccggt tctggtgcaa
      721 atgactacgc gacactcacg ggtacgggta gcgccggtgt acgcgcaaaa gtgcgatctg
      781 tcaatggcgg tgaaaattat ggcattcaaa tcggtaatgt actttttaca cggtattccg
      841 gcgcatggaa aatcactatc gggagttatt cccaaacgac atcggcgatg ccttccggga
      901 cagactggct tgccatcgtg agagattccg tgtcgctgtt atacgtggac ggtaaacaga
      961 tttttaatgt ccggaagtgg aataccggag gtaatgtcag tttttatgtt ggctctttta
     1021 ataccgtcgc agggtttgat gattttttaa tgttcagaga tcccggtgtc gccttgagtt
     1081 acctagatgg tagcggcaag gttgtccaat ctcagttttt agacagcgta ggtactgtcg
     1141 tttcggccac tttatatgat ccactcaatc gtgatgccat cgtcaccaaa tcggctaagt
     1201 acgactccat gtttgcattt cgtaaatcat ttgtgtccgg atttaattgg tcgagcggca
     1261 ttatgacggg atatataacg actacaggca atgcgggcag ttacccttac agccgactaa
     1321 agtttgaaga ctcaccgctg tcgggagtta tcgaatcttc ggcccccgga gatacgttca
     1381 ggatcggacg taaaactgta aaatcggcat atggctctaa tgtaaccggc gacggatttc
     1441 gtgatgacct tcctgcc
//
LOCUS       DAOQNQ010000501         1448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_74028_length_1448_cov_5.176597, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000501 DAOQNQ010000000
VERSION     DAOQNQ010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74028_length_1448_cov_5.176597"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PK754_16775"
     CDS             <1..289
                     /locus_tag="PK754_16775"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HNB11210.1"
                     /translation="VKVGDRLETIARVVYVGKTSMVVRIDAYAENPLSGKRKHCTTAY
                     FNMVALDEDGAPTTIPPLIVETDEEKRDFELAKHIKEAATERKKRFADTSL"
     gene            350..>642
                     /locus_tag="PK754_16780"
     CDS             350..>642
                     /locus_tag="PK754_16780"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNB11211.1"
                     /translation="MIPTLFEIPIFGGIPIHSFGLMMAAAFLTSSFVLRRLFKNAGYS
                     EELSEKIILVAAIAGLAGSKLYYLLFEAFERFKKYPMEMLFSGAGLTWYGGFF"
     assembly_gap    643..660
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <661..1186
                     /locus_tag="PK754_16785"
     CDS             <661..1186
                     /locus_tag="PK754_16785"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNB11212.1"
                     /translation="TISIIYLLKKNKIPVLRGVDLIAIPLPLGYGIGRIGCHLSGDGD
                     YGLPWDGPWATNYSRGVVPPSQAFHGTEIARQYPNGIVPDNTLCHPTPLYETLISTGI
                     FLILWHMSKRKLPAGSIISLYLMLAGMERFFIEFMRLNPKVAFGLSGAQLISVMMITV
                     GASSLAYLWKRATA"
     gene            1183..>1448
                     /locus_tag="PK754_16790"
     CDS             1183..>1448
                     /locus_tag="PK754_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HNB11213.1"
                     /translation="MIRFVYVLFLILVLNDTVSAQANGKIASSRFTIARLKYGGGGDW
                     YGNKNALNHLLRYLSDNTNIAVEKTETYVDPSDIRLFDYTFLYMA"
BASE COUNT          343 a          297 c          364 g          426 t
ORIGIN      
        1 ggtcaaagtc ggagaccggt tggaaacaat tgcgcgggtg gtatatgtcg gtaaaacttc
       61 gatggtcgtt cgtattgacg cctatgccga aaatccgtta tccggaaaac gaaaacattg
      121 tacgacagcc tattttaata tggtcgcgct ggatgaagac ggtgcgccga cgacgatacc
      181 gccgttgatc gttgaaacag atgaggaaaa acgcgatttt gaactggcca aacacatcaa
      241 agaggcggcg actgaacgta agaaacgatt tgctgatacc tcgttgtgat gggtggcgta
      301 tagtatttat tagtatcaac aaataattta cataaaaaag ttatagccca tgattcctac
      361 gttatttgaa atacctatct tcggcggcat tcccattcac agttttgggt tgatgatggc
      421 ggcggctttt cttacttcca gttttgtgtt gcggcggttg ttcaaaaatg ccggatattc
      481 cgaagaattg tcggaaaaaa tcattcttgt cgcagcgatt gccggtttag ccggttccaa
      541 attgtattat cttctttttg aagcgtttga gcgttttaaa aaatatccga tggagatgct
      601 gtttagcggc gcgggactta cctggtatgg cggttttttt ttnnnnnnnn nnnnnnnnnn
      661 gacgataagc attatctatt tgttaaagaa aaataaaatc cccgtcttgc gcggcgtgga
      721 tcttatagcg atcccgttac cgctcggtta tggtatcggc cgtatcggat gccatctttc
      781 gggcgacggc gattacggtt taccgtggga tgggccttgg gctacaaatt attcacgcgg
      841 cgtcgttccg ccttcacagg cctttcacgg cacggagatc gctcgccaat atccgaacgg
      901 gatcgttccg gacaataccc tttgccaccc gacgccgtta tatgaaacgc tgatttcaac
      961 gggaattttt ttgatcctgt ggcatatgag taaacgtaaa ttgcctgcgg gttccatcat
     1021 ttcgctctat ctcatgctcg ccggtatgga gcgttttttt atcgaattta tgcgtctcaa
     1081 tcctaaagtg gcgtttggcc tgagcggcgc gcaattgatc agtgtgatga tgattacagt
     1141 cggtgcgagt tcgctggctt atttgtggaa gcgtgcaacg gcatgatacg gtttgtttat
     1201 gttttatttt tgatcttagt gttaaacgat accgtatcgg cgcaggccaa tgggaaaatc
     1261 gcgtcatctc gttttacgat cgcgcgcctc aaatacggcg gcggcgggga ttggtatggc
     1321 aataaaaacg cattgaatca cctgctgcgt tatttgagtg ataataccaa tattgccgtt
     1381 gaaaaaacgg agacctacgt ggatccgtcg gatatccggc tttttgatta tacctttttg
     1441 tatatggc
//
LOCUS       DAOQNQ010000502         1382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_81482_length_1382_cov_2.824416, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000502 DAOQNQ010000000
VERSION     DAOQNQ010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81482_length_1382_cov_2.824416"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PK754_16795"
     CDS             complement(<1..380)
                     /locus_tag="PK754_16795"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNB11214.1"
                     /translation="MRIVVTGGAGFLGSHLCDRLLAEGHEVIAIDNLITGSVDNISHI
                     RDKRFTFIHYDVTNYIYVEGPVDFVYHFASPASPIDYLELPIQTLKVGSLGTHKALGL
                     AKSKGAGFLLASTSETYGDPLINP"
     gene            complement(391..>1382)
                     /locus_tag="PK754_16800"
     CDS             complement(391..>1382)
                     /locus_tag="PK754_16800"
                     /inference="COORDINATES: protein motif:HMM:NF017142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNB11215.1"
                     /translation="LGMDSWSFADAAGYNSFMSLTAHEYFHLWNVKRIRPKELGPFDY
                     TSENYTKLLWIAEGFTAYYDDLITRRAGYYTVDQYLRRIEGNIQEVQSAPGRFVRSVE
                     EASWDAWIKYYMPDENSVNSSISYYTKGALLGLMLDMEILRATRGEKSLDDLLRWLWT
                     EYDQKLGRGFTPEEFQKAAERLAGTSLDVFFQNYVRGTAELDYNAALQTVGLRVKEIK
                     SDAPYYGFETRNNGEFTIRTVVRDAPAWHAGLNVNDEVLAVDGIRVTARTLQARLNEK
                     KIGTSVRILIARDTQLQEIVITPSEMPARYRLERVSEPTAQQEQIFKKWLNLN"
BASE COUNT          356 a          338 c          324 g          364 t
ORIGIN      
        1 tgcggattga tcaacggatc gccatatgtt tccgaagtcg aggcgagcaa aaaaccggcg
       61 cctttgcttt tggccaaacc cagcgctttg tgtgtcccga gcgagcctac tttgagcgtc
      121 tgaatgggta attctaaata atcaatcggg cttgcggggc ttgcaaaatg gtacacgaaa
      181 tccacagggc cttcgacgta aatatagttg gtcacatcat aatggataaa cgtgaatcgt
      241 ttatcgcgga tgtgcgaaat attatccacg ctgccggtga tcagattgtc aatggcgatg
      301 acttcatgtc cttcggccaa aagccggtcg caaagatggg agcctaaaaa accggcgccg
      361 ccggtaacga ctattcgcat agcggtgatc ctaattaagg tttaaccatt ttttaaaaat
      421 ttgttcttgt tgtgccgtcg gctcacttac gcgctctaaa cgataccgcg ccggcatttc
      481 ggagggcgtg atgacgattt cctgaagttg ggtatcccgt gctattaata tgcggacgga
      541 ggttccgatc ttcttttcat tcagacgagc ttgcaaagtt cgcgcggtta cacgaatacc
      601 gtccacggcg agcacttcat cgttcacatt gagtcctgca tgccatgccg gagcatctct
      661 aacaacggta cgtatcgtaa attcaccgtt atttcgcgtt tcaaaaccat aatacggggc
      721 atcggatttg atttctttta cgcgcaagcc gaccgtttgt aatgccgcat tataatctaa
      781 ttcggccgta ccgcgaacgt agttttgaaa aaacacgtcg agggatgttc cggccaaacg
      841 ttcggcggct ttttgaaatt cttccggtgt aaaaccacgc ccgagtttct gatcgtattc
      901 agtccaaagc caacgcagca aatcatccaa acttttttcg ccgcgcgtgg cacgcaggat
      961 ttccatatcc agcatcaggc cgagcaaggc gcctttggtg taatatgaaa tggacgaatt
     1021 caccgaattt tcatccggca tgtaatattt gatccatgca tcccaactgg cttcttcgac
     1081 actgcgaaca aaacgtccgg gtgcggattg gacttcctga atattacctt cgatgcgacg
     1141 caaatactga tctacggtat aatatcccgc acgccgtgtg atcagatcat catagtatgc
     1201 ggtaaatcct tcggcaatcc ataacagttt agtgtaattt tctgacgtat aatcaaacgg
     1261 gccgagttct ttcgggcgga tacgtttgac attccaaaga tgaaaatatt catgcgcggt
     1321 gagcgacata aagctattat aacctgccgc atcggcaaaa gaccacgaat ccatgccgag
     1381 cg
//
LOCUS       DAOQNQ010000503         1375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_82317_length_1375_cov_3.076515, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000503 DAOQNQ010000000
VERSION     DAOQNQ010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82317_length_1375_cov_3.076515"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PK754_16805"
     CDS             <1..268
                     /locus_tag="PK754_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11216.1"
                     /translation="RVLSGDSLRNVIQAMNVAVGQGFTDSHMIQPVIIGEAKLMVEAK
                     LIGSAHGDCVLALTVPEDESSEKREKIFKKIEAGVRRITASAKA"
     gene            278..>1375
                     /gene="ispG"
                     /locus_tag="PK754_16810"
     CDS             278..>1375
                     /gene="ispG"
                     /locus_tag="PK754_16810"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HNB11217.1"
                     /translation="MEPRTAIPQILNSSKLFRYSEYRFTYRRRPTREVMVGDIGIGGP
                     NPIRIQSMTTSDTLDTEATIRQVIDLVNVGCEIVRITAPSIKDAENLGAIREGLRKRG
                     INVPLVADIHFTPNAALKAAEYVEKIRINPGNYADKKKFAVIEYSENDYTRELERIEE
                     TFKPLVLKCKERGVAMRIGTNHGSLSDRIMNRYGDTPLGMVESALEFVRICEHYDYRD
                     IVLSMKSSNTQVMIAAYRLLAARMYELGMDYPFHLGVTEAGDGEDGRIKSALGIGTLL
                     EEGIGDTIRVSLTEDSIHEIPAARAIADKFNRQNVIFDTEKAALEIRLKEPRDPFQYH
                     RRWTQVVETAALNAGGVHHVRVALPLRSDIHD"
BASE COUNT          375 a          312 c          347 g          341 t
ORIGIN      
        1 ccgtgtgttg tcgggcgatt cgcttcgtaa tgtcatccaa gcaatgaatg tagccgtagg
       61 acaaggattt accgattcgc atatgattca acctgttatc attggtgagg ccaaacttat
      121 ggttgaggcc aaactcatcg gtagcgcaca tggcgactgc gtgttggctt tgactgtccc
      181 ggaagacgaa agttcggaaa aacgagaaaa aattttcaaa aaaatcgagg cgggcgtccg
      241 gcgtattacg gcgagcgcaa aggcgtaatt atttggcatg gaacctcgga cggctatccc
      301 gcaaatatta aactcaagta aactctttcg ctatagtgaa taccggttta cctaccgtcg
      361 gcgacctacc cgcgaggtca tggtcggcga tatcggaatc ggtggtccca atcccattcg
      421 aatccaatcc atgacgactt cggatacatt agataccgaa gcgacaatcc gtcaagtcat
      481 tgatcttgtg aatgtaggct gtgaaatcgt tcgcattacc gcgccatcga tcaaagatgc
      541 cgaaaatctg ggtgcgatac gcgaaggact gcgtaaacgc ggtatcaatg tcccgttggt
      601 tgcggacatt cactttactc ctaatgcggc tttaaaagcc gccgagtatg tagaaaaaat
      661 acgtataaat cccggtaact acgcggacaa gaagaaattt gccgtgatcg aatattccga
      721 aaatgattat actcgtgaac ttgaacgtat cgaagaaacc ttcaaaccgt tggttctcaa
      781 atgcaaagaa cgcggtgtcg caatgcgtat cggcaccaat cacggttcgt tatccgatcg
      841 aataatgaat cgctatggcg ataccccgct tggtatggtg gagtccgcgc tcgaatttgt
      901 tagaatttgc gaacactacg attatcgcga tattgttctt tccatgaaat cgtcgaatac
      961 gcaggtgatg atcgccgcgt accgtctgct tgccgcgcgg atgtatgaat tgggcatgga
     1021 ttaccctttt catcttggcg taacggaagc cggcgacggc gaagacggtc gtatcaaatc
     1081 agcgctaggc ataggcacat tattggaaga gggtatcggt gacacgatac gtgtttcgtt
     1141 aaccgaagat tcaatacacg aaataccggc cgcccgcgct atagcggata aatttaatcg
     1201 tcagaacgta atttttgata cagaaaaagc tgccttagaa attcgtctaa aagaaccgcg
     1261 ggatccgttt caatatcatc gaagatggac gcaggttgta gaaacagcgg cgcttaatgc
     1321 cggcggtgtg catcatgtgc gtgttgctct cccgctgcgg agtgatatcc atgat
//
LOCUS       DAOQNQ010000504         1362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_83899_length_1362_cov_3.791125, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000504 DAOQNQ010000000
VERSION     DAOQNQ010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83899_length_1362_cov_3.791125"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="PK754_16815"
     CDS             complement(<1..1089)
                     /locus_tag="PK754_16815"
                     /inference="COORDINATES: protein motif:HMM:NF036774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HNB11218.1"
                     /translation="MKNIYYLWALTWLCAPGIWAITSDEYGKVIKELAAKIQSSNTTG
                     KPQRLAVVTFVPTKTDAENKNEFGEYITESLISALGRTKQFRLFERRRLDVVLKENAL
                     MMSDLMNSEQAQKVGELIPIDVLFSGTYTKLKNYIDVNCRLIDVITGEIYFSFSGSIA
                     MTADLASLFPGEHNVNAASRAITEAERCKQNADLLKHKLNDLTTSSKIQSAVDEAVRI
                     PFDTECGNVHYDVLYVFTRYKIQNTAYKKFLIQTLGGIANPDQDDRSKETIRYFAADS
                     VIDEEEWRIGLETITRMRNYRLYICLNLLFPTDDLRFSPRVDAYLNRALDNKIGLPKP
                     ISFNHALDHLADGFKDTPTLMSYAYKNYQ"
     gene            1241..>1362
                     /locus_tag="PK754_16820"
     CDS             1241..>1362
                     /locus_tag="PK754_16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HNB11219.1"
                     /translation="MTSKDFEKQALAHTESLYNTALRMTHNPQDAEDLVQEAFY"
BASE COUNT          387 a          269 c          292 g          414 t
ORIGIN      
        1 ctgataattt ttgtaagcat aagacattag agtcggtgta tctttaaaac cgtcggctaa
       61 atgatccaac gcatgattaa acgaaatggg ctttggtaaa cctattttat tgtcgagcgc
      121 ccgattcaaa taggcatcga cacgtggtga aaatctaaga tcatcggtag gaaagagaag
      181 gttgagacaa atgtataaac gataatttct catccgtgtt atcgtttcta atccgatacg
      241 ccattcttct tcgtcaatca cgctgtctgc tgcaaaatag cgtatggttt ctttggagcg
      301 atcatcctga tcgggattgg cgatgccccc caacgtttgt atcaaaaatt ttttgtaggc
      361 cgtgttttga attttatacc gcgtgaagac atacaataca tcataatgca cgttaccgca
      421 ttcggtgtca aaaggtattc gtacggcttc atcaacggcg gactgtattt tggatgacgt
      481 ggtaagatca ttgagcttat gttttaacaa atccgcattt tgtttgcatc gttcagcttc
      541 ggttatcgcc ctggatgcgg cgtttacatt gtgttcgcct ggaaataacg aggccagatc
      601 agccgtcatg gcgatgctac ccgaaaaact aaaatagatc tcgccggtaa tgacgtcaat
      661 caatcggcaa ttaacatcta tgtagttttt aagtttggta tatgtgcctg aaaataaaac
      721 gtctatcgga ataagttcgc cgactttttg cgcctgttcg ctattcatca ggtcgctcat
      781 catcaaagca ttttctttta aaacgacgtc caatcgccgg cgctcaaaaa gtcgaaattg
      841 tttggtgcgg cctaaagccg atatgagcga ttcggtgata tattcaccaa attcattttt
      901 attttcagca tcagttttgg tcggtacaaa agtaactaca gctaaacgct gcggtttacc
      961 ggttgtatta gaggactgta ttttggcggc aagctccttg atgactttgc cgtattcatc
     1021 cgaggtaata gcccaaattc cgggcgcaca gagccacgtg agtgcccaca agtaataaat
     1081 gtttttcatg caattttttg ttaataggta cgctatgata taaatcaata ttggatcagc
     1141 gcaaaatatc ggtattagaa actatttgca agttcatgta gaaaaaagta gttttacagg
     1201 aataattgac atttcaatac gttctatttt ttggcagctc atgacgtcta aggattttga
     1261 aaagcaagca ctcgcacata cggaatcgct gtataatacg gcgctacgta tgacgcacaa
     1321 tccgcaggat gctgaagatt tggtgcaaga ggcattttac aa
//
LOCUS       DAOQNQ010000505         1344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_86283_length_1344_cov_5.888285, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000505 DAOQNQ010000000
VERSION     DAOQNQ010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86283_length_1344_cov_5.888285"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PK754_16825"
     CDS             complement(<1..331)
                     /locus_tag="PK754_16825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNB11220.1"
                     /translation="MYDGLNSIAVKRKGGGTTEYVYGPTGLIASKVNGTWYFVLKDHL
                     GSARTIVDQSGTTKASIDYAPYGAILRTSGSPNIAYQFTGQEYDSTSGLWNYKARMYD
                     AELGVFYA"
     gene            complement(573..1229)
                     /locus_tag="PK754_16830"
     CDS             complement(573..1229)
                     /locus_tag="PK754_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11221.1"
                     /translation="MGIMDDTIHSSIKKKKILMKKQQFILSFICVLSLGACVITRYHD
                     DFWDPTLILKLQDFKVNRNGYFKLINPPPINKYDGFRDFILYENGFFAGHGFRVSQDR
                     MQKAEFKTPQVDWGIYNIGKDSVIIIQHFYPAVIIPGFFSAIDEYAVSSVKGYVSDAG
                     TILIYSRTEHVLRPKKTDEKISHLTFQPPLEFKFIGHDSLPSSDGNWLEEWLKKKKEY
                     "
BASE COUNT          412 a          297 c          230 g          405 t
ORIGIN      
        1 cggcataaaa cacacccagc tccgcgtcat acatccgtgc cttataattc cacaatccgg
       61 acgtgctgtc gtattcctgt ccggtaaact gatacgcgat attgggcgag cccgatgtgc
      121 gaaggatcgc tccataaggc gcatagtcta tcgaagcttt cgtcgtgccc gattgatcca
      181 cgatcgtacg cgccgaaccc agatgatctt tgagtacaaa ataccacgtc ccattgacct
      241 tcgatgcgat cagtcccgtc gggccataga cgtattccgt agtaccaccc cctttgcgtt
      301 ttacggcgat gctattaagg ccatcgtaca aatatatgaa gacccgactt caaatcttaa
      361 ggataaacaa ctttccaatc tcggtctttc aaatcctgca aacttaggtt ataacgttta
      421 agtacttcgt attccgaacg aatgttttcc cacggagact tgctccagaa tatgtacaat
      481 gaaatataaa aaattggcgt atcaattgat gtaaagaaat atcgagttat gatttcatcg
      541 gcaattgtaa ataacaacaa accattattc tatcaatatt cctttttctt ttttaaccat
      601 tcctcaagcc aatttccatc cgatgatggt agcgaatcgt gcccaatgaa tttgaattcg
      661 agcggtggct gaaatgtaag atgactgatt ttttcatcgg ttttttttgg tcttaataca
      721 tgttctgtgc gagagtatat tagaatagta ccagcatccg aaacataccc ttttacagat
      781 gacaccgcat attcatcgat cgcgctaaaa aaaccaggaa ttatgaccgc cgggtaaaaa
      841 tgctgaataa taataacact gtccttgcca atattataaa ttccccaatc cacctgagga
      901 gttttaaact ctgctttttg catacgatct tgtgaaacac gaaacccatg acctgcaaaa
      961 aaaccatttt catataaaat aaaatcacga aatccatcat atttattaat tggaggagga
     1021 tttattaact taaaataacc atttctattc actttgaaat cttgcagttt tagtatgagc
     1081 gtcggatccc aaaaatcgtc gtgatatcgt gtaatcacgc atgctcctaa tgatagcacg
     1141 caaataaacg ataaaatgaa ttgttgtttt ttcattaata tcttcttttt tttaatagat
     1201 gaatggattg tatcgtccat gatacccata aactgatccg taattacctg gtcgaaaata
     1261 aggagctttt cctcctaaaa gccttaataa ccctttgtga gcaactacat tttggaataa
     1321 atcctgcacc catggctgat tcag
//
LOCUS       DAOQNQ010000506         1338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_87189_length_1338_cov_2.342946, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000506 DAOQNQ010000000
VERSION     DAOQNQ010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87189_length_1338_cov_2.342946"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PK754_16835"
     CDS             complement(<1..189)
                     /locus_tag="PK754_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11222.1"
                     /translation="MQIQLSPESSEIRVKDRIQIPEQVHNVGEPVKLAFYLHAALTVT
                     GVQDATIEVDDDEIALKSR"
     gene            441..>1338
                     /locus_tag="PK754_16840"
     CDS             441..>1338
                     /locus_tag="PK754_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter Tol"
                     /protein_id="HNB11223.1"
                     /translation="MKQQNFTAYALSSLLMPWIFLSASSVNASDFITDEHQLSVKEKR
                     AGEAYYRADSKWMIYQAEVADDNPFYQIFLKNIESGSVTQVSPGAGKTTCAWVHPAQE
                     KVLFSSTHDDAQAKAKMIEEIERRKAGEQKSYAWDYDEYYDIYETDFDGKNIRNLTNT
                     LGYDAEASWSPDGKLIAFASNRRAYSGELSEEEQALFKANPASMIDIYIMDADGSNVK
                     RLTHTKSYDGGPFFSPDGKRIVWRRFSDSGREAEIFTMNIDGSDQKQITRLNVMSWAP
                     FYHPSGKYIIFATNLHGHRNFEL"
BASE COUNT          346 a          305 c          353 g          334 t
ORIGIN      
        1 acgggattta agtgcaatct catcgtcatc cacttcgatt gtcgcatcct gcacaccggt
       61 gaccgttagc gcagcgtgca aataaaaagc cagcttcacc ggttcgccca cattatgtac
      121 ctgctcagga atttgaatcc ggtccttgac gcggatttcg gaggactccg gcgacaactg
      181 gatttgcatg gtgtggtgga aggtggatgc ggtggctgtg gcaatccggg caccgaacaa
      241 tatgatgaaa aacaaaaaaa gcgttatgac gtgttttgag gatagtttca ttcttcttat
      301 caaccgggga aaagtatcgg gtgccagtgt cgaaacaaag tttattgttg tctgcacgct
      361 atgatagcgt atgtttactt tccttttgcg ggtgtgttta aatgccttac atcatttgaa
      421 caacttcctt ttgaattcct atgaaacaac agaattttac agcttatgcg ctaagcagcc
      481 ttttaatgcc gtggatattt ctatccgctt cttcagtgaa tgccagtgat ttcatcaccg
      541 acgaacatca gctctccgtc aaggagaaac gcgccgggga ggcttattat cgtgccgatt
      601 cgaagtggat gatttatcag gcggaagtgg cggatgacaa tccgttttat cagatctttt
      661 tgaagaatat agagtccggg tcggtgacgc aggtatcgcc gggggccggc aaaacaacat
      721 gcgcatgggt acatcctgcg caagaaaaag tgctattttc atcgacgcac gacgatgcgc
      781 aagccaaagc caagatgatc gaggaaatcg agcgccgcaa agccggtgag cagaagtcgt
      841 atgcatggga ttacgatgag tattacgata tttacgaaac cgatttcgat ggcaagaata
      901 tacggaacct gaccaatacg ctgggttacg atgccgaggc atcgtggtcg ccggacggga
      961 aattgatcgc gtttgcctcc aaccgccgcg cgtatagcgg ggagttatcc gaagaagaac
     1021 aagcgctgtt caaagccaat ccggcatcga tgatcgatat ttacatcatg gacgcggacg
     1081 gttccaacgt gaagcgattg acgcacacca aaagttacga cggcgggccg tttttcagtc
     1141 ccgatggcaa acgcatcgtg tggcggcgtt tctcggacag cggacgggag gcggaaattt
     1201 tcacgatgaa tatcgacggc agcgatcaga agcagatcac tcgtttgaac gtgatgtcct
     1261 gggcgccgtt ttatcatccg tccggcaaat acattatctt tgctaccaat ttgcacggtc
     1321 atcgcaattt tgaactgt
//
LOCUS       DAOQNQ010000507         1333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_87806_length_1333_cov_4.538341, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000507 DAOQNQ010000000
VERSION     DAOQNQ010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87806_length_1333_cov_4.538341"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="PK754_16845"
     CDS             complement(<1..1316)
                     /locus_tag="PK754_16845"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HNB11224.1"
                     /translation="MAAERGLSVDEDKFTEEMKAQKQRSREEGKKIFINKDIKWETLG
                     FEVKTKFIGYDVLESQAKPLKYYRDGKLIQMVFDQTPFYAESGGQVGDKGTVSIGNHV
                     FEIWDTQRVGDDVVHFADDFENFSLSSSASAQLSVSKDHREPTIKNHSATHLLHSALR
                     SVLGEHVHQSGSVVEPYRLRFDFSHFEKITDDQLKKIETMVQERVFENIALQHHRNIP
                     IADAKKMGALSFFGDKYGEFVNVVQFGEYSKEFCGGTHVPSTGKIGFFKIVSESSIAS
                     GVRRIEAITGSVAEGLFQNQYALQESLRHTLNCKPEEISERIESLINDRKLLEKELSD
                     LRLQSVTNQLDGLMASAVMVNGFKVITRKITLPSGTELRAVGDQLREKLASGVGVLAT
                     QDTILCVVSDDLIRDKKLHAGNLVKAIAQIAGGSGGGRPHSATAGI"
BASE COUNT          336 a          342 c          275 g          380 t
ORIGIN      
        1 ttaatgccgg ccgttgccga atgcggtctc cccccgccgc ttccaccggc tatttgtgca
       61 atagctttaa ctaaatttcc ggcgtgcagt tttttatctc ggatcagatc atcactgacc
      121 acgcaaagga tcgtatcttg ggtggcgagg acgcctactc cggaagccag tttctcacgc
      181 agctgatcac ccaccgcgcg caattccgtt cctgaaggca atgttatttt acgagtaatt
      241 actttaaatc cgttaaccat aacggcggac gccatcaaac cgtctaattg gtttgtcaca
      301 ctttgcagac gaagatccga cagttctttt tcaagaagtt tacgatcatt gatcaaagat
      361 tcgatgcgtt ccgagatttc ttccggttta caattgagcg tgtgccgtaa tgattcttgc
      421 aatgcatatt gattttggaa taatccctcc gcgacggatc cggtaatggc ttcaatccgg
      481 cgcacaccgg aggcgatgct ggattcggac acgattttga aaaaaccgat tttccccgtc
      541 gagggcacgt gcgtgccgcc gcaaaattct ttgctgtatt cgccaaactg caccacgttg
      601 acaaattcac cgtacttatc tccgaaaaac gataacgcac ccattttttt cgcatcggcg
      661 atcggtatat tgcgatggtg ctgcaaagca atgttttcaa atactctttc ctgcaccatg
      721 gtttcgattt tcttcaactg gtcatccgtg attttttcaa aatgcgaaaa atcaaatcgt
      781 aaacgataag gctcaactac ggaaccggac tgatgaacgt gttcaccgag aactgaacgc
      841 agtgccgaat gcaataaatg cgtggccgaa tgatttttga tggtcggctc gcgatgatct
      901 ttggaaacgg aaagctgtgc ggatgcggat gacgataatg aaaaattctc aaaatcatcc
      961 gcaaaatgta cgacatcatc acccacacgt tgcgtatccc aaatctcgaa tacatggttt
     1021 ccgatcgaca ccgtgccttt gtcgccgact tgaccaccgg actccgcgta gaaaggcgtt
     1081 tgatcaaata ccatttgtat caatttacca tcacggtaat acttcaacgg tttggcctga
     1141 ctttccaaaa catcataccc gatgaatttg gttttaacct cgaatccgag cgtttcccat
     1201 tttatgtctt tgttgataaa tatttttttt ccttcttcac gcgaacgttg tttttgcgcc
     1261 ttcatttcct ccgtaaactt atcttcatct acagaaagac cgcgctcggc cgccatcaca
     1321 cgcgtcatat ccg
//
LOCUS       DAOQNQ010000508         1331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_88082_length_1331_cov_4.135580, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000508 DAOQNQ010000000
VERSION     DAOQNQ010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88082_length_1331_cov_4.135580"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..>1331
                     /locus_tag="PK754_16850"
     CDS             76..>1331
                     /locus_tag="PK754_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11225.1"
                     /translation="MKSTIMYSLLCLIYFSPLAAQDEQGEPFIRNYFPQEYRAQSQNW
                     SIVQDHRGLMYFGNNDGVLEFDGIRWRLIPAPNRTSIRALAVDEVGTIYVGAVGEFGI
                     LKTDSTGIRQYYSLSASLDSSFKGITDIWEISCIADKIYFRSNKYFFVFRRSADGSYH
                     FVKAIISDTQFHRSYLIHNTIFVRQDKRGLYIIDQDTLHLLPYGKEFANLRIYGMLPF
                     KDGRFLIATREKNLFVATRSGFAAMKTSVDDWLIQHQLYYASKLKNGNMVLGTILGGV
                     VELSEDGSFIRRVDKSSGLLDDNIWATYVDHNGILWLAMNNGIARVEIGSPYSRFSEL
                     HGLRGTVLSLTRWNNTLYVGTSRGVYRLNRYPSSPEKDVPLSSSQEIWKFEPIAGITA
                     QCWSFVDNEVSLFAGTAQGVFRINGQ"
BASE COUNT          341 a          264 c          329 g          397 t
ORIGIN      
        1 cggtattggc actgcgatac acatagacct tccggtaaaa acattatttc acgaaaacta
       61 ccaacacggt tcgtgatgaa atccacaata atgtatagcc tgctctgttt gatttatttt
      121 tcgccgcttg cggcgcaaga tgagcaaggc gaaccgttca tccgtaacta cttcccccaa
      181 gagtatcgtg cacaatcaca aaactggtct attgtacagg atcatcgggg actaatgtat
      241 ttcggaaaca atgacggcgt attggagttc gacggaatcc gctggcgtct gatccctgct
      301 cctaatcgaa cgtcgatccg tgctttggcc gtagatgagg tggggacgat ttatgtcgga
      361 gcggtcggcg agtttggaat actcaaaaca gattcgacgg gtattcgtca atattattcg
      421 ctgagcgcat cactggattc gtcgttcaaa ggaattaccg atatctggga aattagctgt
      481 attgctgata aaatttattt tcgtagcaat aaatattttt tcgtttttcg acgcagcgcc
      541 gacggttcct atcactttgt caaagctatt atatctgata ctcagtttca ccgttcctat
      601 cttatccata atacgatttt tgtgagacag gacaagcgcg gcttatacat aatagatcag
      661 gatacgctgc atctattgcc gtatggtaaa gagtttgcga atttgcgtat ttatggtatg
      721 ctgccgttta aagacggacg gtttctgatc gcaacgcgcg agaaaaatct ttttgttgca
      781 acacgcagcg gttttgctgc aatgaaaact tcggttgatg attggcttat tcaacatcag
      841 ttgtattatg catcaaaact gaaaaacggt aatatggttt tggggacaat tttgggcggt
      901 gttgttgaat tatccgaaga tggtagtttt attcgcagag tagataaaag ttccggatta
      961 ttggatgaca atatttgggc gacgtacgtt gatcacaacg gcatattatg gctggccatg
     1021 aataacggca ttgcgcgggt tgaaatcggc agtccgtatt cgcgatttag tgaattacac
     1081 ggattgcgcg ggactgtgct ttcattgacg cgttggaata atacgcttta tgtgggcacc
     1141 agccgggggg tgtaccgttt gaatcgttat ccctcatccc cggaaaagga tgttccgctt
     1201 tcatccagtc aggaaatatg gaaatttgag ccgatcgccg gtatcacggc gcagtgctgg
     1261 tcttttgtgg ataatgaggt ttcattattt gcgggtacgg cacaaggtgt tttcaggatt
     1321 aacggtcagc a
//
LOCUS       DAOQNQ010000509         1322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_89330_length_1322_cov_4.966062, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000509 DAOQNQ010000000
VERSION     DAOQNQ010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89330_length_1322_cov_4.966062"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /gene="add"
                     /locus_tag="PK754_16855"
     CDS             complement(<1..758)
                     /gene="add"
                     /locus_tag="PK754_16855"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNB11226.1"
                     /translation="MAKKELNRIEVTEELIHRLPKTELHCHLDGSNRVETLIELAKEQ
                     NVQLPFEDPVKLKSYLEVGDKCENLVDYLKGFAITLSVMQEAYAIERTAFELAEDAAK
                     ENVRYLEVRFSPILHINRGLRLTEIVDAVIRGLERAESKYKIKTGIIICGMRNISPET
                     SLRLAELTVAYKGKKVMAFDLAGQEDSYPAKDHRAAFELILKNNVNTTAHAGEAYGPD
                     SIKQAIHDCGAHRIGHGTRLIEDGDLLNYVNDHR"
     gene            complement(804..>1322)
                     /gene="sucC"
                     /locus_tag="PK754_16860"
     CDS             complement(804..>1322)
                     /gene="sucC"
                     /locus_tag="PK754_16860"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802533.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HNB11227.1"
                     /translation="NFDDNALYRHPDLAALRDIDEEDPLEVEASKYNLNYVKLDGNVG
                     CMVNGAGLAMATMDIIKLAGANPANFLDVGGGANPQTVENGFKIILSDPHVKAIFINI
                     FGGIVRCDRIANGVVQAAKNVNIRVPLIVRLKGTNAEEAKEILRKSGIPMLVANELND
                     AADMILQAVKKN"
BASE COUNT          309 a          325 c          312 g          376 t
ORIGIN      
        1 atgcggtgat cattgacata gttaagcaga tcgccgtcct cgataaggcg tgtaccgtgg
       61 ccgatacggt gtgcaccgca gtcatggatc gcttgtttga tcgaatccgg tccataggct
      121 tcgccggcgt gggcggtcgt attgacgtta tttttgagta tgagctcaaa tgccgcacgg
      181 tgatcttttg ccggataact gtcctcttga ccggcaagat caaaggccat gacttttttc
      241 cctttgtagg cgacggtcaa ttcggccagt cgcagagaag tttcagggct gatattgcgc
      301 ataccgcaaa taataatacc cgttttgatt ttatatttgg attcggcgcg ttcgagtcca
      361 cggatcaccg catccacaat ctcggtgaga cgcagtccgc gattgatgtg caggatcggc
      421 gaaaaacgta cttccagata acgtacgttt tctttggccg catcctccgc cagctcaaac
      481 gccgtgcgtt caatcgcata cgcttcctgc atcacgctga gcgtgatggc aaaacctttg
      541 aggtaatcca ccaggttttc acacttatca ccgacttcaa gataagattt taatttgacc
      601 ggatcttcga acggcaattg aacattttgt tctttagcca attcgatcag cgtctcgacg
      661 cgattactgc cgtcaaggtg acaatgaagt tcggttttag gaagacggtg aatcagttct
      721 tccgttactt cgatgcgatt gagttctttt tttgccatgg tatccgccta taaaaattcc
      781 cgcttgcgcg ggaatttggt tttttaattt ttctttaccg cctgcaatat catatcggct
      841 gcgtcgttga gctcattggc cacgagcatc ggtattcccg attttctcag aatttctttc
      901 gcttcttccg cgttggtgcc tttgaggcga acgatcaacg gtacgcggat attgacattc
      961 tttgcagctt gcacgacacc gttagcgata cgatcgcaac gcacaatacc gccaaaaata
     1021 ttgatgaaaa tggcttttac atgcggatcc gacaaaatga ttttaaaacc attttctacg
     1081 gtctgagggt tagcaccgcc gccgacatcc aagaagttag cgggattggc accggccagt
     1141 ttgatgatgt ccatcgtcgc catggccaaa ccggctccgt tgaccataca accgacattg
     1201 ccgtccagtt taacgtaatt gagattgtat ttagacgctt ccacttcgag cggatcttct
     1261 tcgtcaatgt cacgcaaggc ggcaagatcg ggatgacgat acagagcgtt atcatcaaaa
     1321 tt
//
LOCUS       DAOQNQ010000510         1321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_89488_length_1321_cov_5.176145, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000510 DAOQNQ010000000
VERSION     DAOQNQ010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89488_length_1321_cov_5.176145"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1321
                     /locus_tag="PK754_16865"
     CDS             <1..>1321
                     /locus_tag="PK754_16865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11228.1"
                     /translation="ASGGHNGGILQFDGQTWRKITLKDQRNVYSLAVDDTGAIWVGSF
                     GDWGFLKADTSGLMQYHSLAEAFGDSSNKIRQVWRTAVYNGEVFFGSLEGVIRFAKGK
                     LQWIRPTQSFHYMLPTARALWIKDKGVGLMRWDTESQAFVNAHGGDFFKSGIVVAMVS
                     FEPHEYWVVTADDGIYTYNDSTGVVDKLKTSIPLERLKLANAVRMKNEDIFVTSVLQG
                     IFVITRDGRIRMHVDGDDGRGLSDTKFAFESRDGSLWFGHNNGLSVSDFYNPITYIRN
                     EKGINGAVTGVMSIGNKIFAGTTQGVWESEQDGKTPNILKFHKIKGTDFPVYGIDTMY
                     TRIVIYGGDFAILDANGKKLFHTWEFGSRTVVKLPLSENKILAAHRHQLYLIDVKTFS
                     IKQIHRFDDEVVAILLDSVAGNDAVNYWFSYRNRSVRYIRIGRDGAVS"
BASE COUNT          356 a          250 c          343 g          372 t
ORIGIN      
        1 tgccagtggc ggtcataacg gaggcatttt acaatttgac ggtcagacgt ggcgaaaaat
       61 cacactcaaa gatcaaagaa atgtttattc gcttgctgtc gatgatacgg gtgctatatg
      121 ggtaggtagt tttggggatt ggggtttttt gaaagcggac acttcgggtt tgatgcaata
      181 tcactcattg gcggaagcct ttggtgattc gtctaacaag attcgccaag tctggcgaac
      241 ggcagtatat aacggtgaag ttttttttgg ttctctcgaa ggtgtaatac gatttgctaa
      301 aggcaaactt caatggatac gcccgaccca gtcgtttcat tatatgcttc ccacggcacg
      361 tgcattatgg atcaaggaca aaggtgtcgg cctgatgcgt tgggatacgg aaagtcaggc
      421 ttttgtcaac gcgcacggcg gcgatttttt taaaagcggg atcgtggtgg ctatggtgag
      481 ttttgagccg catgagtatt gggtggttac ggcggatgac ggtatataca catacaacga
      541 ttcgacagga gtcgtagata agctaaaaac atcaatcccg cttgagcgac taaaattagc
      601 taatgccgtg cggatgaaaa acgaggacat ctttgttacc agtgttcttc aaggtatttt
      661 tgtcataact cgcgatggcc gcatccggat gcacgtggac ggtgatgatg gtcgtggtct
      721 ttcagatacc aagttcgctt tcgaatcgag agatggaagc ctgtggtttg gtcacaataa
      781 tggtttgtct gtatccgatt tttataaccc tataacgtac atacgcaatg aaaagggaat
      841 caacggagct gttaccggcg ttatgagcat aggtaataaa atttttgcag gtactacaca
      901 aggcgtgtgg gaaagcgaac aagatggaaa aacgccgaat attctcaagt ttcataaaat
      961 caaaggaacc gattttccgg tttacggcat tgataccatg tacacacgga tcgtgattta
     1021 cggcggtgac tttgcgatac tcgatgccaa cggaaaaaaa ctttttcata cttgggaatt
     1081 cggttcgcgt actgtggtaa aattaccgct atccgagaat aaaatccttg cggcacaccg
     1141 tcatcagttg tatttgatcg atgtaaaaac tttttcaata aaacagatac atcgctttga
     1201 tgatgaagtg gtggcaatat tgttggacag tgttgccggc aatgacgccg ttaattattg
     1261 gttcagctat cgtaatcgat ccgtgcgcta cattcgcatc gggcgtgatg gtgcggtatc
     1321 c
//
LOCUS       DAOQNQ010000511         1310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_91137_length_1310_cov_5.456574, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000511 DAOQNQ010000000
VERSION     DAOQNQ010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91137_length_1310_cov_5.456574"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PK754_16870"
     CDS             complement(<1..455)
                     /locus_tag="PK754_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNB11229.1"
                     /translation="MILLTGGAGFIGSAMLRKLNDLGHDKIIVVDNLGTSDKWKNLIG
                     KRFTDYIHKDEFLRLLDNKAFFDDTTAVIHMGACSATTELDADYLMRNNYHYSKRLAR
                     WALARNIRFIYASSAATYGGGELGFTDDDAQMSALKPLNKYGYSKHIFDL"
     gene            complement(726..>1310)
                     /locus_tag="PK754_16875"
     CDS             complement(726..>1310)
                     /locus_tag="PK754_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014733969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNB11230.1"
                     /translation="TRKGVERIIRYAFEFAAKNGHKKVAMADKSNAMTYGHNLWYRCF
                     FEVAKEYPQIQAKHFYIDALAMMMIREPEMFEVIVTNNLFGDIITDIGAQLQGGMGLA
                     ASGNINPNGVSMFEPVHGSAPDIAGKDIANPVASILTAEMMLRHLGFTAQADMIDRAV
                     RVAIQKNKLTKELGGSEGTKACGSFIAEVIRAEN"
BASE COUNT          355 a          300 c          289 g          366 t
ORIGIN      
        1 agatcaaaaa tatgcttgga ataaccgtat ttattcagag gtttgagcgc ggacatttgt
       61 gcatcgtcat cggtaaaacc taattcgccg ccgccgtagg tagccgcgct gctggcatag
      121 ataaaacgta tgttgcgtgc caatgcccaa cgtgctaagc gtttggagta gtgatagtta
      181 tttcgcataa gataatcggc atccagttcc gtcgtggccg agcaggcgcc catatggata
      241 accgctgtcg tatcatcaaa gaatgcttta ttatccagca aacgcaggaa ttcatctttg
      301 tgaatataat ccgtaaaacg ttttccgata aggtttttcc atttatcgct ggttcccaga
      361 ttatccacca cgatgatctt atcatgcccc agatcgttga gcttacgtaa catggcgcta
      421 ccgataaaac ccgcgccgcc ggtgagaagg atcataaaaa aattcctctg aaaattttca
      481 ggggaatatc taaaattagc aatgcattat caacggtaat actatagcag attaataccg
      541 gatgagacgc ctgtttttga tggaaatttg tatgtgcggt ttaaccgcaa gcgtaaaacc
      601 ctccggttac caccacgagg atgagtaacc acaggattaa ttccctgaat tcctttttca
      661 tacggcccct aattacttta cataagtgta cgatacatac acggtcgaaa gagaaagtaa
      721 atcttctaat tttcagcgcg aatcacttca gcaataaaac taccgcaggc ttttgttcct
      781 tcgctaccgc ccagttcttt ggtcagtttg tttttttgta tggcgacgcg tacagcgcgg
      841 tcgatcatat cggcctgagc agtaaatccg agatggcgaa gcatcatttc ggctgtcaga
      901 atggaagcca cagggttggc gatatcttta ccggcaatgt ccggtgcgct accgtgtacg
      961 ggttcgaaca tggaaacgcc attcggatta atattacccg aagctgcaag gcccatgccg
     1021 ccttgaagct gcgctccgat atccgtaatg atgtcgccga ataaattatt agtaacaatg
     1081 acttcaaaca tttccggttc acggatcatc atcatggcga gtgcatcaat ataaaaatgt
     1141 ttggcctgaa tttgcgggta ttctttggcc acttcaaaga agcaacgata ccaaaggtta
     1201 tgaccatatg tcattgcatt gcttttgtcc gccatggcga cttttttatg tccgttttta
     1261 gcggcgaatt caaacgcgta acggataatg cgctccacgc ctttgcgtgt
//
LOCUS       DAOQNQ010000512         1263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_98354_length_1263_cov_6.658113, whole genome shotgun sequence.
ACCESSION   DAOQNQ010000512 DAOQNQ010000000
VERSION     DAOQNQ010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98354_length_1263_cov_6.658113"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1263
                     /locus_tag="PK754_16880"
     CDS             <1..>1263
                     /locus_tag="PK754_16880"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB11231.1"
                     /translation="AAAKAKRINPRAEVVLFEQGEHISYGSCAMPHFIGDVIPEAEKL
                     IHFTPASFEKEKGCAVQIFHRAEAIWPHRRRVVMRNLQTDQIAEYTYDALIIATGARA
                     RVPDPAWFRYDNVFAVRSLSDSMRIKNYLASRRPSTAVIIGGGFIGMEMAEALRRHAM
                     HVTVLHKSEWPMDSLELSGRRVVADELKRQGVKFEGNITSPELVAGGQTVRAVISGSA
                     RFDADVVIIATGFEPNTDLAREAKIRCGASGGIIVDHHLKTNADRIWAAGACTEFKNA
                     LINKSMYLPLANIANKMGWIAGENAAGGFAEFPPVVRNTAVKIFDLEVASVGLDAASA
                     SAHGLSVIEETIMAHSRVRAYPGAKPVMITLLADKHSKRLIGANLIAEEGAALRANIL
                     ALAIQQKMTLRQIADMQMMYTPPFAPVWD"
BASE COUNT          312 a          307 c          336 g          308 t
ORIGIN      
        1 gcagcggcca aagccaaacg gatcaatccc cgcgccgaag tcgttttgtt tgagcaaggc
       61 gaacatattt cctatggctc atgtgcgatg ccgcatttta tcggtgatgt gataccggaa
      121 gcagaaaaac taattcattt tacaccggcc tcgtttgaaa aagaaaaagg gtgtgccgta
      181 caaatttttc atcgtgcgga agcgatatgg ccgcatcgcc gtcgtgtcgt catgcgcaat
      241 ctgcaaaccg atcagatcgc ggaatacact tacgatgcgt tgattattgc gacgggtgcg
      301 cgagcccgtg tacccgatcc ggcttggttt cgatatgata atgtatttgc cgtacgttca
      361 ctgtccgata gcatgcgtat caaaaattac ctcgcatcac gacgtccttc gacggctgtc
      421 attatcggcg gtggctttat cggtatggag atggctgagg cgttacgccg tcatgccatg
      481 cacgtcaccg tcctgcataa atcggaatgg ccaatggact cactggaatt gtccggtcgg
      541 cgtgtggtgg cggatgaatt aaaacgtcaa ggtgtaaaat ttgaaggtaa tattacttca
      601 ccggaattgg tagcgggcgg tcaaacagta cgtgcggtga tttccggcag cgcccgtttt
      661 gatgccgatg tggtcataat agcgacaggt tttgaaccta acacggatct cgcgcgggaa
      721 gccaagatac ggtgcggtgc ttccggcggt atcatcgtag atcatcacct caaaacaaat
      781 gcagatcgta tatgggcggc cggggcttgt actgaattta aaaatgcact tattaataaa
      841 tcgatgtatc tgccattggc caatatcgcc aacaaaatgg gttggatcgc cggagaaaat
      901 gcagcgggcg gatttgccga gtttcctccg gtggtgcgta atacagctgt taaaattttc
      961 gacctcgaag tagcttcggt aggtctcgat gcggcttcgg catccgcgca cggtctatcc
     1021 gttatcgaag aaacaatcat ggcgcactcc cgcgttagag cgtatcccgg cgctaaaccg
     1081 gttatgatca cacttctcgc cgataaacat tccaaacgtc tgatcggggc caacctcatc
     1141 gccgaagaag gcgccgcgct gcgtgccaac atattggcgc tagcgatcca acaaaaaatg
     1201 acgctgcgtc aaatcgcgga tatgcaaatg atgtatactc cgccgtttgc accggtatgg
     1261 gat
//
LOCUS       DAOQNQ010000513         1247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_100994_length_1247_cov_4.996644, whole genome shotgun
            sequence.
ACCESSION   DAOQNQ010000513 DAOQNQ010000000
VERSION     DAOQNQ010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100994_length_1247_cov_4.996644"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PK754_16885"
     CDS             <1..318
                     /locus_tag="PK754_16885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB11232.1"
                     /translation="GFRVLRAHGGREALVMMHGATIDLVLLDLMMPGMSGFEVVEAMR
                     NEEKLREIPIIILTAKDLTAEERERLRMHVRALMQKATYTTEDLIEEIRRIHPKTGAL
                     SNS"
     gene            315..695
                     /locus_tag="PK754_16890"
     CDS             315..695
                     /locus_tag="PK754_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB11233.1"
                     /translation="MIKQKTILLVEDNEVNMRLVERTIKNPAYTIVSAGNGEEALERA
                     AQYKPDLIILDIQIPKMDGYAVARTLKADPALKHIPIIALTAHAMKGDEEKILAAGCD
                     SYVSKPIEVSKFREKINDLLSDKS"
     gene            complement(726..>1247)
                     /locus_tag="PK754_16895"
     CDS             complement(726..>1247)
                     /locus_tag="PK754_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNB11234.1"
                     /translation="FDLWILQERLQSRVAGLKFFNVFGPNEYHKGDMRSLVNKAFYQV
                     LESGKIRLFKSHRPNYADGAQVRDFVYIKDTVDIMAWLLEHRDVGGIFNLGTGKARSW
                     NDLAKAVFHAMNKQVLIEYFEMPEIIRNAYQYHTEATMTKLQEAGCPIRFRSLEESVK
                     DYVQNYLSSNTYL"
BASE COUNT          383 a          258 c          281 g          325 t
ORIGIN      
        1 gggttccgtg ttttgcgtgc acacggtggc agagaggcgc ttgttatgat gcacggcgcg
       61 acgattgatc tggtgctgct ggatctgatg atgccgggaa tgtccggttt tgaagtagta
      121 gaagccatgc gtaatgaaga aaagctgcgc gaaataccga ttattatttt gacagccaaa
      181 gacctgacgg ccgaagaacg cgagcggctg cgtatgcatg tacgtgcatt gatgcaaaaa
      241 gcaacataca caacggaaga tttaatagaa gaaattcgaa gaatccaccc gaaaacagga
      301 gcgctgagta attcatgata aagcaaaaaa caatactgct ggtggaagac aatgaagtca
      361 atatgcggct tgtggaacgt acgatcaaaa atccggctta tacgatcgta agcgccggta
      421 acggtgaaga agccttggag cgtgctgcac agtacaaacc ggatttgatt attttggata
      481 ttcaaattcc taaaatggac ggttatgcag tcgcgcgaac tctcaaagcc gatccggcgt
      541 taaaacatat tcccattatt gcgttgacgg cgcacgcgat gaaaggggat gaggaaaaaa
      601 tcttagcggc agggtgtgac agctacgtca gcaagccaat cgaggtgtca aaatttcgcg
      661 aaaaaattaa cgacctcttg tccgataaat cctaatagtt ttaatagata ctacaaaatt
      721 aaatattata aatacgtgtt acttgaaaga taattctgca catagtcttt gaccgactcc
      781 tcaagtgatc gaaatcgaat cggacaaccc gcttcttgta gttttgtcat ggttgcttcc
      841 gtatggtatt gatatgcatt gcgaataatc tccggcatct caaaatactc aatcaatact
      901 tgtttgttca tagcatgaaa cactgccttt gcgagatcat tccacgaacg tgcctttcct
      961 gtcccaagat taaaaattcc acctacatca cggtgttcta agagccaagc catgatatct
     1021 accgtatctt tgatgtatac aaaatcgcgg acttgagcgc catccgcgta attcggccga
     1081 tgcgatttaa agagtcgaat tttcccgctt tctaaaactt gataaaaagc tttgtttacg
     1141 aggctacgca tatcaccttt gtgatattcg tttggaccaa acacgttaaa aaactttagg
     1201 ccggctacac gggattgtaa acgttcttgt aaaatccaaa gatcaaa
//
LOCUS       DAOQNQ010000514         1235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_103097_length_1235_cov_3.606780, whole genome shotgun
            sequence.
ACCESSION   DAOQNQ010000514 DAOQNQ010000000
VERSION     DAOQNQ010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103097_length_1235_cov_3.606780"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PK754_16900"
     CDS             complement(<1..893)
                     /locus_tag="PK754_16900"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNB11235.1"
                     /translation="MTNESYPEKIGRYQILSLLGEGAMAKVFKAVDTRIDRVVALKML
                     QFREGMTDEMIQQLKDRFVHEAKFAGKLTHPNIVTIYDAEEENGVSYMAMEYVDGRTL
                     ESVIENNETLTVEQILDILIQLAHGLDYAHKHDIIHRDIKPGNIILTSEDIPKIMDFG
                     IAKITTSSTTVIGTILGTPGYMSPEQITGKSVDHRTDIFALGAVFYELLTREKAFPGK
                     NLTEIMYRVVSENPMPPSMVNLNVPEIINPIVMRAIAKGPGERYQSGTEFAKALEVTK
                     KQLSSGGHRTTISKSVPPSAD"
     gene            complement(928..>1235)
                     /locus_tag="PK754_16905"
     CDS             complement(928..>1235)
                     /locus_tag="PK754_16905"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNB11236.1"
                     /translation="YVVDGRDIGTVVFPNADIKFYMTANVEERARRRLKEMADTDEWD
                     LPKMIEDIQKRDHADMTRAEAPLRQPPDAIVIDNSLMTLDEQIQAIITHIENRFGNQ"
BASE COUNT          299 a          288 c          284 g          364 t
ORIGIN      
        1 ttatcggcag acggcggtac acttttagaa atagtcgtcc ggtggccgcc cgatgataat
       61 tgctttttgg tcacttccag cgctttggca aattcggttc cggattgata acgttctccc
      121 ggacccttgg cgatggcccg catgacgata ggattgatga tctccggtac attgagattg
      181 accatgctgg gcggcatggg attctcgctg accacgcgat acatgatctc cgttagattt
      241 tttccaggaa aagctttttc gcgcgtaagc agttcgtaaa aaacggcgcc taaagcaaag
      301 atgtctgtcc gatggtctac cgatttgccg gttatctgtt ccggtgacat atatccgggc
      361 gttcctaaaa tcgtaccgat caccgtcgta ctggacgtag tgattttagc aatgccgaaa
      421 tccatgattt taggaatatc ttccgatgtg aggatgatat tgccgggctt gatatcgcga
      481 tgtataatat catgtttatg ggcatagtct aaaccatgcg cgagctggat caggatatca
      541 agaatctgct caaccgtaag ggtttcgttg ttttcgatga cggattccaa tgtccggcca
      601 tccacatact ccattgccat atacgatacg ccattttctt cttcggcgtc atagatggta
      661 actatattcg gatgagtcag tttacctgca aatttggctt cgtgcacaaa acgatccttg
      721 agttgctgga tcatttcatc ggtcatgcct tcacgaaatt gcaacatttt caaagccacg
      781 acacggtcaa tacgcgtatc cacggcttta aaaactttag ccatggctcc ttcacccaaa
      841 aggctaagta tctgatacct tccgattttt tcagggtagg attcgtttgt cataggctcg
      901 ttaaaataaa ttcaaaaaaa ctcagaatta ttgattacca aaacggtttt cgatatgtgt
      961 gatgatggcc tgtatttgtt catccaaggt catgagtgaa ttgtctatta cgatcgcgtc
     1021 gggaggttgg cgcagaggcg cttcggcgcg agtcatatcg gcgtgatcgc gtttctgtat
     1081 atcttcgatc atcttgggca aatcccattc atcggtgtcc gccatttctt tcaaacgtcg
     1141 gcgcgcacgt tcttcgacat tggccgtcat ataaaacttg atatccgcat tggggaaaac
     1201 caccgttccg atatcacgcc catctacgac gtaac
//
LOCUS       DAOQNQ010000515         1218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_106111_length_1218_cov_3.774721, whole genome shotgun
            sequence.
ACCESSION   DAOQNQ010000515 DAOQNQ010000000
VERSION     DAOQNQ010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106111_length_1218_cov_3.774721"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PK754_16910"
     CDS             complement(<1..366)
                     /locus_tag="PK754_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017150079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB11237.1"
                     /translation="MSKKPKTILCVDDEHDILDILTYNLKKEGYQVYTADNGDKAITM
                     AEKIRPDMVLLDIMMPGKDGLEVCKALRANPHLKDAHVIFLTAKADEVDEIIGLEFGA
                     DDYVTKPFSAEELLFRIQAV"
     gene            complement(372..1043)
                     /gene="phoU"
                     /locus_tag="PK754_16915"
     CDS             complement(372..1043)
                     /gene="phoU"
                     /locus_tag="PK754_16915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNB11238.1"
                     /translation="MKAQFTEQLGQLEKLVIEMGSVVESQVNNVVTALLTHNSELAQT
                     VIKQDETVDTLEMQIDEAVLNLLALHQPVAMDLRDIIGISKINGDLERIGDHALNIAQ
                     SVLVINERNYRGIHAQISKMADITREMLKDAIDSFIHKDADQAIAVCRRDDTIDDINK
                     FMFADVLAGMIKDPQSIPISMEIIRISKNFERMADLCTNIAEDVVFIRNAKSIKHQKE
                     LLQPK"
     gene            complement(1095..>1218)
                     /locus_tag="PK754_16920"
     CDS             complement(1095..>1218)
                     /locus_tag="PK754_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HNB11239.1"
                     /translation="VSDKTAFMWMGELIEFNATKKIFTSPEKKMTEDYITGRFG"
BASE COUNT          295 a          279 c          250 g          394 t
ORIGIN      
        1 gacagcttgt atgcggaata ataattcttc tgcgctgaat ggtttggtca catagtcatc
       61 ggcaccaaat tcaagcccga taatttcatc cacttcatcg gctttggcgg tcagaaaaat
      121 aacgtgcgca tctttaaggt gcggattcgc tctcagcgct ttacacactt ccaatccgtc
      181 tttgcccggc atcatgatgt ccagtaaaac catatcgggt cgaatttttt ccgccatggt
      241 gattgcttta tcgccattgt ccgcggtata tacctggtat ccttcttttt tgagattgta
      301 cgttagaata tccaggatat catgttcgtc gtcaacacac aggatggttt ttggtttttt
      361 ggacatacat tttactttgg ttgtaatagt tctttctgat gtttgataga ctttgcatta
      421 cgaataaaca caacgtcctc ggcaatattg gtacaaagat cagccatacg ctcgaaattt
      481 ttactgatgc gaatgatctc catcgaaatc ggaatactct gcgggtcttt gatcattccg
      541 gccaatacat ccgcaaacat gaacttattg atatcgtcaa tcgtatcatc acgtcggcat
      601 accgcgatag cctgatcggc gtctttgtga ataaaactgt caatcgcatc tttgagcatt
      661 tcccgcgtaa tatcggccat tttggatatc tgtgcatgga tgccgcggta attgcgttca
      721 ttgatcacca gcacggactg cgcgatattg agcgcatgat cgccgatacg ttcaagatca
      781 ccgttgatct tggaaatacc gatgatgtcg cgtaaatcca tcgctacagg ctgatgcaaa
      841 gcgagcagat taagcacggc ttcgtcaatt tgcatttcca acgtatcgac tgtttcgtcc
      901 tgtttgatca cggtctgggc taattccgaa ttgtgcgtca gcaacgctgt cacgacgtta
      961 ttgacctgcg attcaacgac gctgcccatt tcgatcacaa gtttttcaag ttggccaagc
     1021 tgttccgtaa actgagcttt catgtattac aatcctttat tctcaatact tttcatggtt
     1081 aggtttatgt ttttttatcc gaagcgaccc gtgatataat cttcggtcat ttttttctcc
     1141 ggggaagtaa atattttttt ggtggcgtta aattcgatta actcgcccat ccacataaat
     1201 gccgttttat ccgatacg
//
LOCUS       DAOQNQ010000516         1200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_109447_length_1200_cov_5.186900, whole genome shotgun
            sequence.
ACCESSION   DAOQNQ010000516 DAOQNQ010000000
VERSION     DAOQNQ010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109447_length_1200_cov_5.186900"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PK754_16925"
     CDS             <1..695
                     /locus_tag="PK754_16925"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNB11240.1"
                     /translation="EVLDETSVYAFIRNSLQGISPRLFKLIKITKKQWKHKALTEFRA
                     VLPWPEDKLVQYLMASGIDSAYAVKRIKEIPDAMREDALANRIYRPAFARWCAGQQAN
                     IVLPDTLTMRRNEKFTAETFGRLVDLARHNHSDIMLAAIPSPFAVVQKEKLVWAVSSP
                     PETVQEQIRNLVIRMCKENQTAYIDLKPALKAADQAGETTHFAYDLHWTARGHRLVAD
                     TLFERIKDRVIN"
     gene            706..>1200
                     /locus_tag="PK754_16930"
     CDS             706..>1200
                     /locus_tag="PK754_16930"
                     /inference="COORDINATES: protein motif:HMM:NF018917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HNB11241.1"
                     /translation="MLTQLLRGTSYPFTAFGFIVHHHLLRWVLAPFLINVLLFGAFFV
                     WTIIHIGDVIHWMETGFLGEMNIVLFWIVAAIMLTIYVLIMAYIWSLIGGLVAAPFNQ
                     YLSVQSEHIEGLVSDHTTSWWHDLYRALVAELKKISFLLFLITPLVLLPLMLLHLIPG
                     FGSVL"
BASE COUNT          305 a          262 c          290 g          343 t
ORIGIN      
        1 gagaggtatt agacgaaacg agtgtttatg catttatccg gaattcatta caaggaatat
       61 cgccgcgatt attcaaactg atcaaaatta ccaagaagca atggaaacac aaagcactca
      121 ctgaattcag agcggtgtta ccttggccag aagataaact cgtacaatat ctgatggcat
      181 ccggtataga cagtgcgtac gctgtaaaac ggataaaaga aattccggat gcaatgcggg
      241 aggatgcgtt ggccaatcgt atctaccgac cggcctttgc gcgatggtgt gctgggcaac
      301 aagccaatat tgttttaccc gatacgctga ccatgcgtcg taacgaaaag tttacggcgg
      361 aaacgtttgg gcgtttggtt gacttggcgc gtcacaatca ttcggatatc atgctggcgg
      421 ccataccgtc gccttttgcg gttgttcaga aagagaagtt agtttgggct gtttcatcac
      481 ctcctgagac ggtacaggag caaatcagga atctggtcat tcggatgtgt aaagaaaacc
      541 aaacggctta catagacttg aaacctgctt tgaaggctgc cgatcaagcc ggtgaaacga
      601 cacattttgc ctatgatctg cactggacgg caaggggaca tcgtttggtc gccgatacgt
      661 tatttgaacg cattaaagat cgcgtcatca actaatagag aacccatgct aactcaatta
      721 ttacgcggca caagttatcc ttttacggcg ttcggtttta tcgttcatca ccatctcctg
      781 cgatgggtgt tagcaccgtt tttgatcaat gtgttgcttt tcggtgcgtt tttcgtatgg
      841 acgatcatac atatcggcga tgtcatacat tggatggaaa ccggttttct cggtgaaatg
      901 aatattgtgc ttttctggat tgtcgcagcg atcatgctga cgatctatgt actgatcatg
      961 gcctacattt ggagtttgat cggcggatta gtcgctgcac cttttaatca gtacctcagt
     1021 gtacaatcgg aacatattga aggtctcgta tccgatcata ccacttcgtg gtggcatgac
     1081 ctctaccgcg cgttggtcgc cgaactgaaa aaaatttcat ttcttttgtt tctgataacg
     1141 cctttggttt tactgccgct catgttgctc catttgatac ccggcttcgg aagcgtgctc
//
LOCUS       DAOQNQ010000517         1175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_114420_length_1175_cov_5.019643, whole genome shotgun
            sequence.
ACCESSION   DAOQNQ010000517 DAOQNQ010000000
VERSION     DAOQNQ010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114420_length_1175_cov_5.019643"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PK754_16935"
     CDS             <1..484
                     /locus_tag="PK754_16935"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNB11242.1"
                     /translation="RSLTIIGWSLIGLAVILALAEVVAKHRRRIADMDFKDTQIIGLA
                     QAVALIPGASRSGVTITAGLFLGLTRESAARFSFLLSIPAVLASGLFELKDLIKYGLG
                     GEELIHLFVATLVAGIVGYASIAFLIKWLQTRSTTIFIVYRIVLGILILALSGMHVIR
                     "
     gene            658..>1175
                     /locus_tag="PK754_16940"
     CDS             658..>1175
                     /locus_tag="PK754_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11243.1"
                     /translation="MKPTHLLFRRIFLTAFMIAAVVLVVVNFYRFAASPTDENIFDDA
                     PSQLYIFRSFPADISYYQKRDTTGVFIRDSVYAGDLLVSIGGILWKDQDSIARFLKRV
                     GSEEKINAIVFRSKASKMFVANFIRKDAPDSFYHEIQFTAHVREVAHNGASDRAGMLV
                     GDLIVRINGRTF"
BASE COUNT          265 a          271 c          287 g          352 t
ORIGIN      
        1 tcgtagcctc accatcatag gatggagcct gatcggactg gctgtgatct tagcattggc
       61 ggaggtggtg gccaaacatc gtcgtcgtat tgccgacatg gattttaaag acacccagat
      121 catcgggctt gcgcaagcag ttgcattgat accgggcgca tcgcgttcgg gggtgacgat
      181 cacggccgga ttatttctcg gtttgacgcg tgaaagcgcc gcacgatttt cgtttctact
      241 ttcgatccct gccgtgctag ccagcgggct tttcgaacta aaagacctta ttaaatacgg
      301 cctcggtggc gaagagttga ttcatctttt cgtggccacg cttgtcgccg gtatcgtcgg
      361 atatgcttcc attgcttttc taatcaagtg gctacagaca cgttcgacga cgatttttat
      421 cgtttatcgc atcgttctcg gcatattgat tttagcacta tcgggaatgc atgtgattcg
      481 atagaatgaa ttgtaatgta tcggtatgct ctgcgcggcg gtaatcggaa cgtattccgg
      541 cgggcatgaa ccggatgcgc atataaatag tgaatctttt gttatcatag cgcgtaatct
      601 ttgtaagagt gttttttgac tcttgcagca ttgcgtccta agccataagt tatgaccatg
      661 aagcccacac acctcctatt tcggcgcatt tttctgacgg catttatgat cgctgccgta
      721 gtcttagtcg ttgtcaattt ttatcgtttt gccgcttctc cgacagacga aaatattttt
      781 gatgatgcgc cgtctcagct atacatcttt cgcagttttc cggccgatat cagttattat
      841 caaaaacgcg atacgacagg tgtatttatt cgcgattccg tatatgcagg cgacttgctc
      901 gtatccatcg gcggtatctt gtggaaagat caggattcca tagcccgatt tttgaaacgc
      961 gtcggatctg aagaaaaaat caacgccatt gtttttcgct ccaaagcatc caaaatgttc
     1021 gtagccaatt ttattcggaa agatgccccg gacagttttt accatgaaat ccagtttacg
     1081 gcgcatgtgc gtgaagtggc ccacaacggc gcttccgatc gcgcgggcat gttggtcggt
     1141 gatttgattg ttcgtatcaa cgggcgaaca ttcaa
//
LOCUS       DAOQNQ010000518         1167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_116096_length_1167_cov_6.527878, whole genome shotgun
            sequence.
ACCESSION   DAOQNQ010000518 DAOQNQ010000000
VERSION     DAOQNQ010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116096_length_1167_cov_6.527878"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="PK754_16945"
     CDS             complement(<1..1123)
                     /locus_tag="PK754_16945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11244.1"
                     /translation="MSVLSGTVYMSDSSRWASSIDSVASGATVSMDNGSHNFDANSKL
                     IGAGNVIVTTTVNMSGTYNITGTTTASSGALLFNGTTGSLLNIGSTLAMSGGSVQVLT
                     GDSLILGSATLNSTSSTLLVKAPIRFTTSFTMNQGVLRATDSTIKLLIGSGQTLTLNP
                     TSTLRWQKITVENNGTIDWTGGGTATLTVDSGLVINNYGLFDCKIASSPSLDHNQGAL
                     SRLNNYGIFRRSVNATNFTVDADIYSYSGSTVDLQTGVTYFYRSLITTDADVTIASGA
                     TLYFTGTSAAFDANSSISGAGNVTISSGTATFTGDVNIKGVSYFLSSSSMTFNASAGK
                     LLTLGDSVSIASGTINIMSPQSITMRSLRQSGGTLNLTMN"
BASE COUNT          323 a          299 c          244 g          301 t
ORIGIN      
        1 cgttcatagt caaattgagc gtaccgccgg attgacgcaa gcttcgcata gtaatgcttt
       61 gcggtgacat gatatttatc gtgccactgg ctatggaaac actatcgccg agcgttagta
      121 attttcctgc agaagcatta aatgtcatac tggacgaaga caaaaaatac gaaacacctt
      181 taatgtttac gtctcctgta aacgtagccg ttccgctgct tatggttaca tttcccgcac
      241 cgctgatgga tgaattagca tcaaacgcgg ctgaagtacc ggtaaaatac aatgtagctc
      301 cggaggcaat cgtcacatcc gcatccgttg ttatcagcga acgatagaaa tacgttaccc
      361 cagtttgaag gtcaaccgta gaaccgctgt atgaataaat atctgcgtcc acagtaaagt
      421 tggttgcatt aaccgaacgg cggaatattc cgtagttatt caaacgtgaa agcgcacctt
      481 ggttatgatc caatgacggt gaactggcta tcttacaatc aaataaccca taattattga
      541 tcaccaaacc ggagtccacc gtcagcgtgg ccgtaccgcc tccggtccaa tcaatcgtgc
      601 cattattctc caccgtgatc ttttgccaac gaagcgttga tgtaggattg agcgtaagcg
      661 tctgaccgct gccaatcaac aacttgatcg tcgaatccgt agcacgcaat acaccctgat
      721 tcatcgtaaa cgatgtcgta aaacgtatcg gcgcttttac caataaggtc gagcttgtgc
      781 tattcagtgt cgcacttccc aatatcagcg aatcacccgt aagcacctgc acactaccgc
      841 cgctcattgc aagcgtggaa ccaatattca acagtgaccc cgttgtacca ttgaacaaca
      901 acgccccgct tgatgccgtc gttgttcccg taatattata tgttccactc atattcactg
      961 tggtggtaac aattacattt cccgcgccga tgagttttga attggcatcg aagttatgcg
     1021 atccgttatc catcgaaacc gttgcgcccg atgctaccga atcaatggat gaggcccacc
     1081 gggaactatc ggacatatac actgtaccag aaagaacaga cacatacgta ttggtgtttt
     1141 ccatcatcac cgagatagta cttgtcc
//
LOCUS       DAOQNQ010000519         1160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_117588_length_1160_cov_3.444344, whole genome shotgun
            sequence.
ACCESSION   DAOQNQ010000519 DAOQNQ010000000
VERSION     DAOQNQ010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117588_length_1160_cov_3.444344"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="PK754_16950"
     CDS             complement(<1..179)
                     /locus_tag="PK754_16950"
                     /inference="COORDINATES: protein motif:HMM:NF013898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+ dependent nucleoside transporter N-terminal
                     domain-containing protein"
                     /protein_id="HNB11245.1"
                     /translation="MERFTGLLGVAAIIGIAFLISNNKKRVNWRLVGMGFLIQMVFGI
                     LVIKGHALGFPFNLVA"
     gene            complement(240..569)
                     /locus_tag="PK754_16955"
     CDS             complement(240..569)
                     /locus_tag="PK754_16955"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNB11246.1"
                     /translation="MIEQLLNVGKRGKGALFKELLNLFQKNAPPSIAKIKSFLELRDG
                     LSLSHEAHSLKGSSANLGAKHLAEICQTIEVLGKESRLQEASEKISELDNVYLESLEE
                     LKKLIPV"
     gene            complement(639..1055)
                     /gene="coaE"
                     /locus_tag="PK754_16960"
     CDS             complement(639..1055)
                     /gene="coaE"
                     /locus_tag="PK754_16960"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNB11247.1"
                     /translation="MQRQLLGTFVFSNPERVKQLNAIVHPHLLQAMRARIDEAQKNNP
                     PMILVDAALIYEADIADTFDKVIVVTADEKIRMTRIMTRDQIQSEDAMLRIRAQMPIE
                     EKERRADYVLTNNTSLVDLNRKVEVLFDTLVNNNKR"
BASE COUNT          326 a          277 c          208 g          349 t
ORIGIN      
        1 gctacgagat taaacggaaa tcctaaagca tgccctttga tcacaagtat gccaaagacc
       61 atctggatta aaaaacccat gccaaccaat cgccagttta cccgcttttt attattactg
      121 atcaaaaaag ctataccgat gatcgctgct acaccgagaa gtcccgtaaa tcgctccatg
      181 tgcactcccg aagttattta tttgttaaaa catatctctt taagtctaat ttcgccgacc
      241 taaaccggaa ttaatttttt taactcttcc aaggattcaa gataaacatt gtctaattct
      301 gaaatttttt cagaggcttc ttgcaaacga ctctctttac ctaaaacctc tatagtttga
      361 caaatttctg caagatgttt tgcgcctaaa ttggcgctgg aacctttgag gctgtgtgct
      421 tcatgactta aactcaatcc gtcacgcagt tccagaaatg atttaatttt tgcaatactg
      481 ggcggcgcat ttttttgaaa caaatttaat aattctttga aaagcgcacc cttcccacgt
      541 ttaccgacat tcagcaattg ttcgatcatt gcgtattcga tgagatccgt gcgatgatgt
      601 ggaacagcca tgcgttaaga tgagttttaa atataaattt atcgcttgtt attattcacc
      661 aacgtatcaa ataatacttc tacttttcga ttcagatcaa ccaaagatgt attattggtc
      721 aacacataat cggcgcgacg ttctttttct tcgatcggca tttgtgcgcg tatgcgcaac
      781 atcgcgtctt ccgattgaat ctgatcccgc gtcatgatcc gtgtcatccg tatcttttca
      841 tccgccgtca cgacgatcac cttgtcaaag gtatcagcta tatcggcttc atagatcaat
      901 gcggcatcca ccaatatcat cggcggattg tttttttgcg cctcatcaat ccgagcacgc
      961 atggcttgca gtaaatgcgg atgcacgatg gcgttgagtt gtttgacacg ttcaggattt
     1021 gaaaaaacaa atgtacccaa cagttgtctt tgcaaacgtc cctaaaaatc aaaaaaagct
     1081 tcaccaaacg cgggtcgtat ttccgttcga accgccgcat ccgactccac gacatgacgg
     1141 ccaagccgat cggcatcaat
//
LOCUS       DAOQNQ010000520         1142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_121587_length_1142_cov_3.912603, whole genome shotgun
            sequence.
ACCESSION   DAOQNQ010000520 DAOQNQ010000000
VERSION     DAOQNQ010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121587_length_1142_cov_3.912603"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PK754_16965"
     CDS             complement(<1..505)
                     /locus_tag="PK754_16965"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNB11248.1"
                     /translation="MKKTFIVVLLLCAQYQSYAQTAAPYTVRVQLVDSDGKKVAYANV
                     YFADGDFAGAFTNDSGLAIIRTHIAGERHLTASLIGYDTEVKKIKVPHAQTMHVTMHE
                     KSVELGELVIEASAYGGGEGKVAVSKIDVYTTPGGAADVFQSVKVLPGVTQTDETAAV
                     PVRGGSPS"
     gene            complement(522..>1142)
                     /locus_tag="PK754_16970"
     CDS             complement(522..>1142)
                     /locus_tag="PK754_16970"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB11249.1"
                     /translation="RAAMADPQNVWPLYWSAFAYYRLAIMKLYGGEPDEDAGKQYIET
                     GLSLLEKANTLKPGHAESLALTGSLTGMKIRFNPLSAMWLGPKSQEYMSKAVEADPKS
                     PRVFYLIGIGTMNTPSMFGGGADKATGDFLKAIRLYEEEAANPPADPLAPTWGYDECY
                     SFLGSAYEAQKMFNEAKSCYQKSLTINPESRRAKAQLMRLENKNTQ"
BASE COUNT          271 a          307 c          255 g          309 t
ORIGIN      
        1 cggaaggact gccgccgcgt acgggaacag ccgctgtttc atccgtttgt gttacacccg
       61 gcaaaacctt gacgctctga aaaacatcgg cggcgccgcc cggcgtggta tatacatcta
      121 ttttagaaac ggcgaccttg ccttcaccgc cgccgtaggc gctggcctcg atcaccagtt
      181 cgcccaattc taccgacttc tcatgcatgg tgacatgcat tgtttgagca tgaggaactt
      241 ttattttctt aacttccgta tcatagccga tcaaactcgc tgtcagatga cgctctccgg
      301 ctatgtgggt gcgaataatc gccaaaccgc tgtcattggt aaacgctccg gcaaaatcgc
      361 cgtcggcaaa atacacattg gcgtacgcca ctttttttcc atcactatcc accaactgta
      421 cacgtactgt atatggcgcg gccgtctgtg cgtaactttg gtattgcgcg catagcagca
      481 gaactacgat aaaagttttt ttcattttta taccggattg attattgcgt gtttttgttc
      541 tcaagccgca tcaattgcgc tttagcccgg cgcgattcgg gattgatggt caatgatttc
      601 tgataacaac ttttagcttc gttaaacatt ttttgtgctt cgtaggcgga tcctaaaaaa
      661 gaataacact catcatagcc ccatgtcggc gccaacggat ccgccggcgg gttggccgct
      721 tcttcttcat acaaacgtat agctttgagg aaatcgcccg tcgctttatc tgcaccgccg
      781 ccaaacatgg acggcgtatt catcgtaccg ataccgataa gataaaaaac acgaggactt
      841 ttgggatccg cttcgacggc tttactcata tactcctgtg atttagggcc gagccacata
      901 gccgataatg gattaaaacg aattttcata cctgtgagag agcccgtcaa agctaacgac
      961 tcggcatgac ccggtttgag cgtattggct ttttccaata gcgacaaccc tgtttcgatg
     1021 tattgtttac cggcgtcttc atccggttca ccaccgtata gtttcataat cgccagacga
     1081 tagtatgcaa atgcgctcca atacaacggc cacacattct gcggatcggc catagccgcc
     1141 cg
//
LOCUS       DAOQNQ010000521         1122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_126177_length_1122_cov_7.076851, whole genome shotgun
            sequence.
ACCESSION   DAOQNQ010000521 DAOQNQ010000000
VERSION     DAOQNQ010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126177_length_1122_cov_7.076851"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /gene="rlmD"
                     /locus_tag="PK754_16975"
     CDS             <1..1042
                     /gene="rlmD"
                     /locus_tag="PK754_16975"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNB11250.1"
                     /translation="PAPLGSPRIFHYRNKMEFTFGDTEWIVDKTSETSPRSFMLGMHV
                     PQRFDKILHIDQCYLQSPLANDILAFVRDFAQRSGLPPYSVQTNEGFWRFLIIRETVH
                     TGHLMVNVMTYSDQPEVMKEFKQAIINRFPQITSLINGITDRKSQVAFAERENLLHGQ
                     SVITEKLDGLEFEISSSSFFQTNTLGAEVLYRTIREMADLKGHEVVLDLYCGTGSIAI
                     YIADRAREVIGIELIESATRNAEKNAQRNRADNCRFINGDMRTTLDTLQTKADVVILD
                     PPRSGLHEDVIRSVMRLGPDKIVYVSCNPSTQARDLALMQADYTIERIQPVDMFPHTY
                     HIENIVQMRKKV"
BASE COUNT          312 a          257 c          271 g          282 t
ORIGIN      
        1 acccgcaccg ctcggatcgc cgcgcatttt tcattaccgt aacaaaatgg agtttacgtt
       61 cggcgatacg gagtggattg tagataaaac atcggaaacg tcgccgcgtt cgttcatgct
      121 cggtatgcac gttcctcaac ggttcgataa aattcttcac attgatcagt gctatttgca
      181 atcgcctttg gctaacgata ttctcgcctt tgtgcgcgat tttgcacagc gtagcggatt
      241 gccgccgtac tccgtgcaga ccaacgaagg attctggcgt ttcttgatta ttcgtgagac
      301 cgtacatacc ggccatttga tggtcaatgt tatgacgtat tcggatcagc cggaggttat
      361 gaaggaattt aaacaggcga tcataaatag gtttccgcaa attaccagtc tgatcaacgg
      421 gattaccgac cgcaaatcgc aagtagcgtt cgccgaacgc gaaaatcttt tgcacggaca
      481 aagcgtgatc actgaaaaat tagacggcct tgaatttgaa atatcatcca gttcgttctt
      541 tcagaccaat acattgggcg cagaggtgct atatcgcacc atccgtgaaa tggcggatct
      601 gaaaggtcac gaagtagttc tggatttgta ttgcggtaca ggcagtatcg cgatatatat
      661 tgccgaccgg gcgcgcgaag tgatcggtat tgaattaatc gaaagtgcga cccgcaatgc
      721 ggaaaaaaac gcccaacgaa atcgcgccga caattgccgg tttattaacg gtgatatgcg
      781 tacaacgctg gacacgttgc aaaccaaagc ggatgtggtg atactcgacc cgccgcgctc
      841 cgggttacac gaagatgtca ttcgaagtgt gatgcgtttg gggccggata aaatagttta
      901 cgttagttgc aatccttcta cgcaagcgcg cgacttggct ctgatgcaag cggattatac
      961 catcgaacgt atacaaccgg tagatatgtt cccgcacaca taccatatag agaatatcgt
     1021 acaaatgaga aaaaaagtat gacgtccaaa cgccacaata tcctctgtgt tgatgacgaa
     1081 ccggtcatat taaatatcct gtcgaaactt ttcgaagaaa ag
//
LOCUS       DAOQNQ010000522         1122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_126248_length_1122_cov_2.929709, whole genome shotgun
            sequence.
ACCESSION   DAOQNQ010000522 DAOQNQ010000000
VERSION     DAOQNQ010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126248_length_1122_cov_2.929709"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..838
                     /locus_tag="PK754_16980"
     CDS             71..838
                     /locus_tag="PK754_16980"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HNB11251.1"
                     /translation="MKIRIFCVYIALSLSCVVLAQVNTENLRKYNADRGFHHTVGFSL
                     GLQEGNSESLSMKSNYRLDWLTSAYYSFFAANYERAESRGKLYSHKGFMHLRVTRNLS
                     DYVIAEGFTQKEFNAFIDLKDRQLIGSGLRYEIMRTDSGRKSPILVWAVGAGLMWERE
                     MYEHNEPTTRKLRSTNYLSIRWNPDDRLAFVATTYYQADTRKFSDYRLLHNTNMSIAL
                     WKKLSFVAAFNARYDHRPAPGIRTYDMELSNGVNIVF"
     gene            842..>1122
                     /locus_tag="PK754_16985"
     CDS             842..>1122
                     /locus_tag="PK754_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11252.1"
                     /translation="MAHKTTKRTLSKKKSKKTTLRKSDSTRQHDALLHLYRLRKGFDS
                     LVRAAVRISADLQSIDRVSVWLFTPDRSAIQCVALYSREKKKYFQGDVL"
BASE COUNT          305 a          226 c          261 g          330 t
ORIGIN      
        1 atattttaca taacaccgat tttattatat attgtccggc ttaccctcct tgtctaaagg
       61 ttctacatat atgaaaatca ggattttttg cgtttatatc gcattgagct tgtcgtgtgt
      121 cgtattggcg caggtaaata ctgaaaattt acgcaagtat aatgcggatc gaggattcca
      181 tcataccgta ggtttcagcc tcggcctcca agaaggtaat tccgaatcac tcagcatgaa
      241 aagtaattac cgcctggact ggcttacctc ggcatattat tctttttttg ccgcgaacta
      301 tgagcgtgcc gaaagccgtg gaaaactgta ttcgcataaa gggtttatgc atttgcgtgt
      361 tacccgaaat ctgagcgatt acgtgattgc cgaaggtttt acgcagaaag aatttaatgc
      421 gtttattgac cttaaagatc gtcaattgat cggcagcggt ctacgctatg aaattatgcg
      481 aacggattcg gggcgcaagt ctcctatatt ggtatgggct gtcggcgctg gattgatgtg
      541 ggagcgtgaa atgtatgaac acaatgaacc gaccacccga aaactgcgtt cgactaacta
      601 tctatccata cgatggaacc cggatgatcg gttggctttt gttgctacga cctattatca
      661 agcggatacg cgcaagtttt ctgactatcg tcttttgcat aatactaata tgagtattgc
      721 gctatggaaa aaattgagtt ttgtggcagc ctttaatgcg cggtatgatc ataggccggc
      781 gcccgggatt cgtacttacg atatggaatt gagcaatggc gtaaacatag ttttttgagt
      841 tatggcgcat aaaaccacaa agagaacatt atctaaaaag aagagcaaaa aaacgactct
      901 tcgaaaatca gatagcacac ggcagcatga tgcccttctg catttgtatc gtctgcggaa
      961 aggttttgac tcgttggtgc gtgccgccgt acgcatatcc gccgatctgc aatctattga
     1021 tcgcgttagt gtttggttat tcacgccgga tcgatcggct atccaatgtg tcgcacttta
     1081 cagtcgggag aaaaaaaaat attttcaagg ggacgtactt tt
//
LOCUS       DAOQNQ010000523         1101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_131175_length_1101_cov_4.113767, whole genome shotgun
            sequence.
ACCESSION   DAOQNQ010000523 DAOQNQ010000000
VERSION     DAOQNQ010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131175_length_1101_cov_4.113767"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PK754_16990"
     CDS             complement(<1..459)
                     /locus_tag="PK754_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11253.1"
                     /translation="MLAELATEIIPRSNTLSIQRVLPHVLCPECRSASFKMYADGSTE
                     LSVLTHGVIVCQSCHHCIRFEDGILEVLKEKPDHLSPAQRSNFFLPVAAGYQKCWRTW
                     CMHMMCGTSFPNEKEAALLISSLQLSSLPSSGIILDLGTSQGFYAVAVAQA"
     gene            619..>1101
                     /locus_tag="PK754_16995"
     CDS             619..>1101
                     /locus_tag="PK754_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNB11254.1"
                     /translation="MGFEMMCLAFAGFILFLIIMSAFFVVKQQTVAVIERFGKFTRMA
                     ESGLRFKIPMVDRIAARVNMRVQQLDVVVETKTQDDVFVHLKVSVQFFVIPEKVYEAY
                     YKLSNVHQQVTSFVFDLVRARVPKLKLDDLFEKKDEIADAVRHELSDTMEDFGYAVLK
                     A"
BASE COUNT          300 a          227 c          282 g          292 t
ORIGIN      
        1 cgcttgagcg accgcaaccg cgtagaaacc ttggctggtg ccgagatcca ggattatgcc
       61 cgacgaaggt aacgatgaaa gttggagcga tgagatcaga agcgcggctt ctttttcgtt
      121 ggggaacgac gtaccgcaca tcatgtgcat acaccacgta cgccagcatt tttgataacc
      181 ggcggcaacc gggagaaaaa aattactacg ttgtgcgggc gaaagatgat ccggtttttc
      241 tttgagcact tcgaggatac cgtcttcaaa acgaatacaa tgatggcagg attggcaaac
      301 gatcacgccg tgcgtcagca ccgataattc cgtagatccg tcagcataca ttttgaatga
      361 tgcggatcgg cactcggggc acaatacatg cggaagtact cgctgtatag ataaagtatt
      421 ggagcgggga atgatttcgg tagccagttc agcaagcatg gagcgatgaa tgcgttcagt
      481 atggatttat ttcattggcg cgaaaatacg cgaattcatt agccaatgtc cattcttttt
      541 tttaaaatga caccattgag gaatttaatc atacaacaat atatcgcgta acgtatccat
      601 taaaacggag attcaaatat gggctttgaa atgatgtgtt tggcgtttgc agggtttatc
      661 ctgtttctga ttatcatgag cgcttttttt gtggtcaagc aacagaccgt tgccgtgatc
      721 gagcgtttcg gaaaatttac acggatggcc gagtcgggtt tacgttttaa aattccgatg
      781 gtggatcgta tcgccgcacg ggtcaatatg cgcgtgcagc aactcgacgt cgttgtggaa
      841 accaaaacac aggatgacgt atttgtgcat ctcaaagtat ccgttcagtt ttttgtgata
      901 ccggaaaaag tgtacgaagc ctattacaag ctatccaatg tgcatcagca agtaacttcg
      961 ttcgtatttg atcttgtgcg tgcgcgcgtg cccaagctca aactggatga tttgtttgag
     1021 aaaaaagacg agatcgccga tgccgtgcgt catgaactta gcgatacgat ggaagatttc
     1081 ggatatgccg tactcaaagc g
//
LOCUS       DAOQNQ010000524         1099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_131666_length_1099_cov_4.013410, whole genome shotgun
            sequence.
ACCESSION   DAOQNQ010000524 DAOQNQ010000000
VERSION     DAOQNQ010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131666_length_1099_cov_4.013410"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="PK754_17000"
     CDS             complement(<1..1026)
                     /locus_tag="PK754_17000"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HNB11255.1"
                     /translation="MLQVIQNYNSGELKVDNVPVPTIQSGGILVRTMYSLISAGTERT
                     TVETAQKSLVGKAKSRPDLVKKVLQVAKRDGVLATLQLVKNKLDTPVPLGYSSAGYVV
                     AVGEGVTEFAVGDRVACAGQGYASHSEVVYVPINLAVKVPEQVPLDEAAYATLGAIAL
                     QGIRQSEVTVGDNVAVIGLGLLGLLTVQMLKSAGCRVVGFDINPNACARAKSMGADYA
                     LSPADDMDAMLQKLTRGYGVDSAIITAATSGNEPIILAGDICRNKGNMVIVGAVPANV
                     PRSPFYEKEIDVRFSRSYGPGRYDVKYEEGGIDYPYGYVRWTENRNMQAFLDLIDAKK
                     IDLQSVTT"
BASE COUNT          277 a          287 c          255 g          280 t
ORIGIN      
        1 ggtagtaacc gactggaggt caattttttt ggcatcgatc agatccaaaa aagcctgcat
       61 gtttcggttt tctgtccagc gaacataccc atacggataa tctattccgc cttcttcgta
      121 cttcacatca tagcgtccgg ggccgtagct ccgcgaaaat cgcacatcaa tttctttttc
      181 ataaaagggg ctgcggggta cattagccgg tacggcgccg acgataacca tattcccttt
      241 attacgacaa atatctccgg caagaatgat cggttcattg ccggacgtgg cggctgtaat
      301 aatcgcgcta tccacaccat acccgcgcgt aagcttttgc aacatagcat ccatatcgtc
      361 ggctggtgac agagcataat cggcgcccat tgatttggcc cgtgcgcacg cattggggtt
      421 gatatcaaaa ccgacaacgc ggcatccggc ggattttaac atctgcacgg tgagaagtcc
      481 cagaaggcca agcccaatca ctgcgacatt gtcaccgaca gtgacttccg attggcgaat
      541 accctgcaaa gcaatcgcgc ccagcgtggc gtaagcggct tcgtccagcg gaacctgctc
      601 cggtactttt acggccagat tgatcggtac atatacgact tccgaatgcg atgcataacc
      661 ttgtcctgcg catgccacgc gatcaccgac ggcaaactcg gtaacgccct ctccgacagc
      721 aacaacataa ccggcagagc tataaccgag cggcaccggc gtatccagtt tatttttgac
      781 caattgcaat gtagccagga cgccgtcccg ttttgcaact tgaagaactt tttttacaag
      841 atccggccga cttttggctt tacctactaa acttttttga gccgtttcga cggtagtacg
      901 ttccgtgccg gcgctgatca gagaatacat cgtgcgtaca agaatgccac cggattgtat
      961 ggttggaacg ggaacattat caactttgag ttcgccggag ttataatttt gaatgacttg
     1021 gagcaaaaat gaattcctat agttcgttta attgcggtct ataaactgtc gataccatat
     1081 ttctaaagta agtagttgc
//
LOCUS       DAOQNQ010000525         1044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_146940_length_1044_cov_3.750253, whole genome shotgun
            sequence.
ACCESSION   DAOQNQ010000525 DAOQNQ010000000
VERSION     DAOQNQ010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146940_length_1044_cov_3.750253"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..888)
                     /locus_tag="PK754_17005"
     CDS             complement(52..888)
                     /locus_tag="PK754_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11256.1"
                     /translation="MDPSARLIHTLIRQRSVVDAVDAGLALCRNRYYDLCVTTLIVFV
                     PLYSLKYVLFVLDLIPFERVFNSFGFFYVQSYTPVNIMVWFLDSAANTLCGAAICRYV
                     TMTAESHTLPIRHTKLLPWGELWPLLFNTFIAELIIIIGFGLCILPGIWTGVMFMFVT
                     PLITYEKLTSFSDIWTRSKYLSQNDWWRMLGFLLLTFLLLAILGLSLTYLFAAVYQLV
                     LGQLAWLSGQTADENLLTMIGGAVVSVLLLPMQMVFTVVLYFDIRARREGLDIERMVQ
                     KL"
     gene            complement(898..>1044)
                     /locus_tag="PK754_17010"
     CDS             complement(898..>1044)
                     /locus_tag="PK754_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018114726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNB11257.1"
                     /translation="AGMSLLIAALCAEGTTTIQNIRQLDRGYERIDERLNALGARIER
                     RKDA"
BASE COUNT          335 a          245 c          235 g          229 t
ORIGIN      
        1 cggcatgtgc ggccatcgta ccggctaata tccaaagaat aaaaatatgt ttcatagttt
       61 ttgaaccatt cgttcgatgt ctaaaccttc acggcgtgca cggatgtcaa aatacaaaac
      121 aacggtaaat accatttgca tcggtaacag cagtacggat acgacggcgc cgccgatcat
      181 agtcagcaga ttttcgtcgg ctgtttgacc gctaagccag gctaactgac cgagaaccaa
      241 ctgatacacg gcagcaaaaa ggtaagtcaa agataaaccg agtatcgcaa gtaaaagaaa
      301 cgtgagcaat aaaaatccaa gcattcgcca ccagtcgttc tgcgacaaat attttgaacg
      361 cgtccaaata tctgaaaaag aagttagttt ttcatatgtg atcagaggcg tcacaaacat
      421 aaacatcacc ccggtccaaa tgccgggtaa aatacataag ccaaatccga tgataatgat
      481 tagttccgct ataaacgtat taaacaggag cggccataat tcaccccacg gcaaaagttt
      541 ggtatgtcgg atcggtaaag tatgcgactc tgccgtcatc gtcacataac ggcagattgc
      601 agcaccgcat agcgtattag cagcgctgtc caaaaaccat accatgatat tgaccggtgt
      661 gtacgattga acatagaaaa aaccgaacga attgaagacg cgttcaaaag gaattaaatc
      721 caacacgaaa agaacgtatt tcagggaata aagcggtacg aacacgatca acgtggtgac
      781 acataaatcg taataacgat ttcggcacag agccaaaccg gcatcgacgg cgtccaccac
      841 ggagcgctgg cgtattaatg tatgtataag tcgcgcagat ggatccatag ttcgaggtta
      901 agcatctttg cgtcgttcga tacgcgcccc cagcgcattg agtcgctcat ctatccgttc
      961 atacccgcga tccagttggc gaatattttg gatcgtggta gtgccttcgg cacaaagcgc
     1021 ggcgatcaga agtgacatac cggc
//
LOCUS       DAOQNQ010000526         1030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0492
            NODE_151215_length_1030_cov_4.841026, whole genome shotgun
            sequence.
ACCESSION   DAOQNQ010000526 DAOQNQ010000000
VERSION     DAOQNQ010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810124
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:57:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,403
            CDSs (total)                      :: 3,357
            Genes (coding)                    :: 3,345
            CDSs (with protein)               :: 3,345
            Genes (RNA)                       :: 46
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151215_length_1030_cov_4.841026"
                     /isolate="AMR_MDS_0492"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(342..>1030)
                     /locus_tag="PK754_17015"
     CDS             complement(342..>1030)
                     /locus_tag="PK754_17015"
                     /inference="COORDINATES: protein motif:HMM:NF036774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HNB11258.1"
                     /translation="REQDFGASDYVNQDQSVQVGRLLNANVIIVGTYQPLKEYILVNT
                     KLVDVETGSILEVMSYEMEIDSRVDNLLGIGDEKLRQEEEKRRQKEYEKSLAKSKDKF
                     FGGGMSGYYSWTSGKPTFKNFMATVDFGLFFRRIIAKAGYSKYTREVDGHYMTFGGDF
                     KVYDYLRFGYLRGVSLKKDETIADNLTGQSSSASIFYWTPIEGDGVSFSILRSTVKGN
                     KLWGLGLSFY"
BASE COUNT          321 a          231 c          199 g          279 t
ORIGIN      
        1 agtgttccag ataatgcgat atgcccgact gaccattttt ttcagtgcga ctgccgactt
       61 caaaccaaac catcacggta catatcggta aacgatgatc ggcattgatc aacagacgcg
      121 aaccgccggg tagcggggtg cttcgtatat caaggttaga acgagccata aattcatttt
      181 atgctgatga acataaacta aaacacagaa gtgtaaaaaa cgaagtacgg taatattgta
      241 gtatagcgga aacttaaact gcgcagtgcg gtgaacaaac atcgcgcatg gaagccgatt
      301 ttatttataa tttaaaaatt aaaaataaat aatgaagcaa actaataaaa actaagtccg
      361 agtccccaaa gtttattgcc tttgaccgta gaccgtagga tcgaaaaact tacgccgtca
      421 ccttcgatcg gcgtccaata aaaaatactt gccgatgagg attgaccggt aaggttatcg
      481 gctatagttt catctttttt caaagagacc ccgcgcaagt aaccaaagcg taaataatca
      541 taaactttga aatcgccgcc aaaggtcatg taatggccat ccacttcgcg tgtatattta
      601 ctatacccgg ctttggcgat gattcgacga aaaaacaaac cgaaatcaac cgtagccata
      661 aaatttttaa atgtaggctt tcccgatgtc caagaatagt agccggacat tcctccgccg
      721 aaaaacttgt ctttggattt ggccaaagac ttctcatact ccttttgacg gcgcttttct
      781 tcttcttgac gcaatttttc atcgccaata cccaaaagat tatccacgcg agagtcaatt
      841 tccatttcgt agctcatgac ctcgagaata ctccctgttt ccacatccac gagtttggtg
      901 ttaacaagta tgtattcttt caaaggttga tatgtaccta cgataataac attggcgttg
      961 agtagtcggc cgacttgcac cgattgatct tgattcacat aatccgatgc gccgaaatcc
     1021 tgttcgcgaa
//
